LOCUS       DAMJWS010000001       110546 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMJWS010000001 DAMJWS010000000
VERSION     DAMJWS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 110546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 110546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..110546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.001"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            328..2562
                     /locus_tag="PC399_000001"
     CDS             328..2562
                     /locus_tag="PC399_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2590881.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2593..3426
                     /locus_tag="PC399_000002"
     CDS             2593..3426
                     /locus_tag="PC399_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2590882.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKKKFDKND
                     Y"
     gene            3666..5399
                     /locus_tag="PC399_000003"
     CDS             3666..5399
                     /locus_tag="PC399_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2590883.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5791..6349
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5860..5888
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6879..6983)
                     /locus_tag="PC399_000004"
     CDS             complement(6879..6983)
                     /locus_tag="PC399_000004"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2590884.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7608..7853
                     /locus_tag="PC399_000005"
     CDS             7608..7853
                     /locus_tag="PC399_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590885.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIVFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8606..8752)
                     /locus_tag="PC399_000006"
     CDS             complement(8606..8752)
                     /locus_tag="PC399_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590886.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8848..9497
                     /locus_tag="PC399_000007"
                     /pseudo
     CDS             8848..9497
                     /locus_tag="PC399_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            10038..11393
                     /locus_tag="PC399_000008"
     CDS             10038..11393
                     /locus_tag="PC399_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2590887.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11511..12179)
                     /locus_tag="PC399_000009"
     CDS             complement(11511..12179)
                     /locus_tag="PC399_000009"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF2590888.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12246..12674)
                     /locus_tag="PC399_000010"
     CDS             complement(12246..12674)
                     /locus_tag="PC399_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2590889.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12781..13374
                     /locus_tag="PC399_000011"
     CDS             12781..13374
                     /locus_tag="PC399_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF2590890.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13493..14428
                     /locus_tag="PC399_000012"
     CDS             13493..14428
                     /locus_tag="PC399_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF2590891.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(14699..15643)
                     /locus_tag="PC399_000013"
     CDS             complement(14699..15643)
                     /locus_tag="PC399_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2590892.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            15961..17391
                     /locus_tag="PC399_000014"
     CDS             15961..17391
                     /locus_tag="PC399_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2590893.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            17591..18175
                     /locus_tag="PC399_000015"
     CDS             17591..18175
                     /locus_tag="PC399_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF2590894.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(18279..19058)
                     /locus_tag="PC399_000016"
     CDS             complement(18279..19058)
                     /locus_tag="PC399_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2590895.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(19116..20372)
                     /locus_tag="PC399_000017"
     CDS             complement(19116..20372)
                     /locus_tag="PC399_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF2590896.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            20630..21529
                     /locus_tag="PC399_000018"
     CDS             20630..21529
                     /locus_tag="PC399_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF2590897.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            21553..23001
                     /gene="ddlR"
                     /locus_tag="PC399_000019"
     CDS             21553..23001
                     /gene="ddlR"
                     /locus_tag="PC399_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF2590898.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            23060..23866
                     /locus_tag="PC399_000020"
     CDS             23060..23866
                     /locus_tag="PC399_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2590899.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            23892..25811
                     /locus_tag="PC399_000021"
     CDS             23892..25811
                     /locus_tag="PC399_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2590900.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(26053..26400)
                     /locus_tag="PC399_000022"
     CDS             complement(26053..26400)
                     /locus_tag="PC399_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF2590901.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(26387..28315)
                     /locus_tag="PC399_000023"
     CDS             complement(26387..28315)
                     /locus_tag="PC399_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF2590902.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(28726..30915)
                     /locus_tag="PC399_000024"
     CDS             complement(28726..30915)
                     /locus_tag="PC399_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF2590903.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            31246..31977
                     /locus_tag="PC399_000025"
     CDS             31246..31977
                     /locus_tag="PC399_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2590904.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEGDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            32057..32692
                     /locus_tag="PC399_000026"
     CDS             32057..32692
                     /locus_tag="PC399_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2590905.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(32943..33536)
                     /locus_tag="PC399_000027"
     CDS             complement(32943..33536)
                     /locus_tag="PC399_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF2590906.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(33607..34470)
                     /locus_tag="PC399_000028"
     CDS             complement(33607..34470)
                     /locus_tag="PC399_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF2590907.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            34633..35802
                     /locus_tag="PC399_000029"
     CDS             34633..35802
                     /locus_tag="PC399_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2590908.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            35903..36379
                     /locus_tag="PC399_000030"
     CDS             35903..36379
                     /locus_tag="PC399_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2590909.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            36945..37739
                     /locus_tag="PC399_000031"
     CDS             36945..37739
                     /locus_tag="PC399_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF2590910.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            37763..38206
                     /locus_tag="PC399_000032"
     CDS             37763..38206
                     /locus_tag="PC399_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF2590911.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(38295..39581)
                     /locus_tag="PC399_000033"
     CDS             complement(38295..39581)
                     /locus_tag="PC399_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2590912.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(39799..40689)
                     /locus_tag="PC399_000034"
     CDS             complement(39799..40689)
                     /locus_tag="PC399_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2590913.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            40981..41325
                     /locus_tag="PC399_000035"
     CDS             40981..41325
                     /locus_tag="PC399_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2590914.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            complement(41484..43580)
                     /locus_tag="PC399_000036"
     CDS             complement(41484..43580)
                     /locus_tag="PC399_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF2590915.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(43593..44753)
                     /locus_tag="PC399_000037"
     CDS             complement(43593..44753)
                     /locus_tag="PC399_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2590916.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            complement(44772..45839)
                     /locus_tag="PC399_000038"
     CDS             complement(44772..45839)
                     /locus_tag="PC399_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF2590917.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            46191..46757
                     /locus_tag="PC399_000039"
     CDS             46191..46757
                     /locus_tag="PC399_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2590918.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(46952..47938)
                     /locus_tag="PC399_000040"
     CDS             complement(46952..47938)
                     /locus_tag="PC399_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF2590919.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(47968..48663)
                     /gene="pxpB"
                     /locus_tag="PC399_000041"
     CDS             complement(47968..48663)
                     /gene="pxpB"
                     /locus_tag="PC399_000041"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF2590920.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(48683..49903)
                     /locus_tag="PC399_000042"
     CDS             complement(48683..49903)
                     /locus_tag="PC399_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF2590921.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(49932..50723)
                     /locus_tag="PC399_000043"
     CDS             complement(49932..50723)
                     /locus_tag="PC399_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF2590922.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            51311..51940
                     /locus_tag="PC399_000044"
     CDS             51311..51940
                     /locus_tag="PC399_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2590923.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRSVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(52054..53568)
                     /gene="glpK"
                     /locus_tag="PC399_000045"
     CDS             complement(52054..53568)
                     /gene="glpK"
                     /locus_tag="PC399_000045"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2590924.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            53812..54354
                     /locus_tag="PC399_000046"
     CDS             53812..54354
                     /locus_tag="PC399_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2590925.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            54368..56860
                     /locus_tag="PC399_000047"
     CDS             54368..56860
                     /locus_tag="PC399_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2590926.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            57281..57679
                     /locus_tag="PC399_000048"
     CDS             57281..57679
                     /locus_tag="PC399_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2590927.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(57896..58105)
                     /locus_tag="PC399_000049"
     CDS             complement(57896..58105)
                     /locus_tag="PC399_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590928.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(58137..58346)
                     /locus_tag="PC399_000050"
     CDS             complement(58137..58346)
                     /locus_tag="PC399_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2590929.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            58645..59064
                     /locus_tag="PC399_000051"
     CDS             58645..59064
                     /locus_tag="PC399_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2590930.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            59292..59747
                     /locus_tag="PC399_000052"
     CDS             59292..59747
                     /locus_tag="PC399_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590931.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(59954..60214)
                     /locus_tag="PC399_000053"
     CDS             complement(59954..60214)
                     /locus_tag="PC399_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590932.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(60477..60830)
                     /locus_tag="PC399_000054"
     CDS             complement(60477..60830)
                     /locus_tag="PC399_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590933.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANVEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(60847..66168)
                     /locus_tag="PC399_000055"
     CDS             complement(60847..66168)
                     /locus_tag="PC399_000055"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590934.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNALISQYT
                     EISLSNVTDAYSSYNATFIVVDEKKVYATGINTNYLLGFSTSDGSNVNLGLLSDWYYI
                     NISGSSYSRVSCTNNITKINNIIIYEYVTVFCTNIGSFLTGYHGTSWTKPTDSSYRVQ
                     YQGISYAGYLDSYIYNYYPTRCTQSSSSTTFAYLYNGESSSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNIFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGLVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(66185..67168)
                     /locus_tag="PC399_000056"
     CDS             complement(66185..67168)
                     /locus_tag="PC399_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2590935.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(67169..67867)
                     /locus_tag="PC399_000057"
     CDS             complement(67169..67867)
                     /locus_tag="PC399_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2590936.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(67864..68916)
                     /locus_tag="PC399_000058"
     CDS             complement(67864..68916)
                     /locus_tag="PC399_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2590937.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(68909..69337)
                     /locus_tag="PC399_000059"
     CDS             complement(68909..69337)
                     /locus_tag="PC399_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2590938.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(69337..69663)
                     /locus_tag="PC399_000060"
     CDS             complement(69337..69663)
                     /locus_tag="PC399_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2590939.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(69679..71208)
                     /locus_tag="PC399_000061"
     CDS             complement(69679..71208)
                     /locus_tag="PC399_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2590940.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(71490..71912)
                     /locus_tag="PC399_000062"
     CDS             complement(71490..71912)
                     /locus_tag="PC399_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590941.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(71912..74365)
                     /locus_tag="PC399_000063"
     CDS             complement(71912..74365)
                     /locus_tag="PC399_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590942.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(74365..74532)
                     /locus_tag="PC399_000064"
     CDS             complement(74365..74532)
                     /locus_tag="PC399_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590943.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(74559..75005)
                     /locus_tag="PC399_000065"
     CDS             complement(74559..75005)
                     /locus_tag="PC399_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2590944.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(75119..75547)
                     /locus_tag="PC399_000066"
     CDS             complement(75119..75547)
                     /locus_tag="PC399_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2590945.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(75561..76625)
                     /locus_tag="PC399_000067"
     CDS             complement(75561..76625)
                     /locus_tag="PC399_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2590946.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(76630..77073)
                     /locus_tag="PC399_000068"
     CDS             complement(76630..77073)
                     /locus_tag="PC399_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590947.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(77322..77762)
                     /locus_tag="PC399_000069"
     CDS             complement(77322..77762)
                     /locus_tag="PC399_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590948.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(77867..78064)
                     /locus_tag="PC399_000070"
     CDS             complement(77867..78064)
                     /locus_tag="PC399_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2590949.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            78233..78553
                     /locus_tag="PC399_000071"
     CDS             78233..78553
                     /locus_tag="PC399_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2590950.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            78635..79141
                     /locus_tag="PC399_000072"
     CDS             78635..79141
                     /locus_tag="PC399_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2590951.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            79842..80597
                     /locus_tag="PC399_000073"
     CDS             79842..80597
                     /locus_tag="PC399_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2590952.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(80740..81168)
                     /locus_tag="PC399_000074"
     CDS             complement(80740..81168)
                     /locus_tag="PC399_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2590953.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(81589..81789)
                     /locus_tag="PC399_000075"
     CDS             complement(81589..81789)
                     /locus_tag="PC399_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2590954.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(81972..83141)
                     /locus_tag="PC399_000076"
     CDS             complement(81972..83141)
                     /locus_tag="PC399_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2590955.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            83377..84198
                     /locus_tag="PC399_000077"
     CDS             83377..84198
                     /locus_tag="PC399_000077"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF2590956.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRHEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(84274..85662)
                     /locus_tag="PC399_000078"
     CDS             complement(84274..85662)
                     /locus_tag="PC399_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF2590957.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGSLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(85797..86555)
                     /locus_tag="PC399_000079"
     CDS             complement(85797..86555)
                     /locus_tag="PC399_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2590958.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFASSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(86557..87273)
                     /locus_tag="PC399_000080"
     CDS             complement(86557..87273)
                     /locus_tag="PC399_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2590959.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(87275..87982)
                     /locus_tag="PC399_000081"
     CDS             complement(87275..87982)
                     /locus_tag="PC399_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2590960.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGQ"
     gene            complement(88098..88634)
                     /locus_tag="PC399_000082"
     CDS             complement(88098..88634)
                     /locus_tag="PC399_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2590961.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(88736..89806)
                     /locus_tag="PC399_000083"
     CDS             complement(88736..89806)
                     /locus_tag="PC399_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2590962.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(89799..90857)
                     /locus_tag="PC399_000084"
     CDS             complement(89799..90857)
                     /locus_tag="PC399_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF2590963.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(90844..91176)
                     /locus_tag="PC399_000085"
     CDS             complement(90844..91176)
                     /locus_tag="PC399_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2590964.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            91375..92496
                     /locus_tag="PC399_000086"
     CDS             91375..92496
                     /locus_tag="PC399_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF2590965.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(92624..94522)
                     /locus_tag="PC399_000087"
     CDS             complement(92624..94522)
                     /locus_tag="PC399_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF2590966.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            94837..95613
                     /locus_tag="PC399_000088"
     CDS             94837..95613
                     /locus_tag="PC399_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2590967.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(95705..96457)
                     /gene="xth"
                     /locus_tag="PC399_000089"
     CDS             complement(95705..96457)
                     /gene="xth"
                     /locus_tag="PC399_000089"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF2590968.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(96618..97568)
                     /gene="galT"
                     /locus_tag="PC399_000090"
     CDS             complement(96618..97568)
                     /gene="galT"
                     /locus_tag="PC399_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF2590969.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            97953..99149
                     /locus_tag="PC399_000091"
     CDS             97953..99149
                     /locus_tag="PC399_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2590970.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(99261..100601)
                     /locus_tag="PC399_000092"
     CDS             complement(99261..100601)
                     /locus_tag="PC399_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2590971.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     HQIAIVITENNGAISNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(100740..101492)
                     /locus_tag="PC399_000093"
     CDS             complement(100740..101492)
                     /locus_tag="PC399_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2590972.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(101640..103217)
                     /locus_tag="PC399_000094"
     CDS             complement(101640..103217)
                     /locus_tag="PC399_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2590973.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     MPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGIIAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYAIVYYNVFKILILRFNLQTPGREEITDINFDLKPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(103629..104081)
                     /locus_tag="PC399_000095"
     CDS             complement(103629..104081)
                     /locus_tag="PC399_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2590974.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(104392..105837)
                     /locus_tag="PC399_000096"
     CDS             complement(104392..105837)
                     /locus_tag="PC399_000096"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF2590975.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(105981..106889)
                     /locus_tag="PC399_000097"
     CDS             complement(105981..106889)
                     /locus_tag="PC399_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2590976.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            107146..107970
                     /locus_tag="PC399_000098"
     CDS             107146..107970
                     /locus_tag="PC399_000098"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2590977.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            108010..108540
                     /gene="lepB"
                     /locus_tag="PC399_000099"
     CDS             108010..108540
                     /gene="lepB"
                     /locus_tag="PC399_000099"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2590978.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(108622..110403)
                     /locus_tag="PC399_000100"
     CDS             complement(108622..110403)
                     /locus_tag="PC399_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2590979.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT        38223 a        17362 c        13013 g        41948 t
ORIGIN      
        1 taaacttaaa attaattata aaaaaactct accttactaa ctatacagct cacaatctat
       61 ctatttatga aaaaatatga tataattgca ttcaaaaagg aaatctttca atatactaga
      121 atttaagcat taactaattt tatctaatat tattactaaa ctaaaaaata tttgtatttg
      181 tagcattgga gcaagtaact taatgtatat tcattttaaa tacttattta aaatttagca
      241 tttaaattat ttacaaatta acatttaaat acttctagca taaatacagt tgtcattaaa
      301 atttaaagta tcttatggag gaataccatg caaaatcata attatataaa aatgttaaat
      361 aaaaaggtag aagtattatc taaggaatta gatagtagaa attttaaatt aaaaacctta
      421 aatgaaatag attttttttc taaaatacca aacagagaat ttatgtacca acatattgat
      481 gactgtattt cgaaaaatat acatatagga atcatgttat taaatatcga taactttaaa
      541 ttatttaatg atatatatgg atacctagtc ggagaaaatt tattaattac tctaactcaa
      601 aacctattgc actttattcc agaatcaaag ggaatagtcg cttatttaaa aggagaaggt
      661 tttttaattg tacttccaaa tgtatcaaat aaagagttaa ataatatggg tgcaactatt
      721 atagagagcg cccgagaatt aaaaataaaa cttcctaatg aaaaagaaat atactccaat
      781 gttacattta gtatcggcag cactatctgg aatggtgaac caaacatgac tactttgatg
      841 cttttcaacc aagtatatag tgctttaaaa aaggctaaaa gtgaaggtaa aaatagatac
      901 attctatttg attttaaaga taatagtttt tacaataaat tagaaaatga taaggagttc
      961 ttatcagaaa tatcacatgt tctacaaact aaacaaattg aagtatttta ccaaccactt
     1021 tataatataa aaaataatac tatagttgga tgtgaggcac ttgcaagatg gagacaccct
     1081 attaaaggac ttcttactcc agataaattt attcctttat ttgaacagtt tggtctgata
     1141 acggaattag atttgcattt atttgaagag aactgtaaaa atataagaag atggattgat
     1201 aacaatgatg tttttgttcc aatttgttgt aacttttctc gtcttcattt ttttaaccct
     1261 aattttccaa gtatgctaaa aggtattact gaaaagtaca gcgtctctac atcaaatttt
     1321 agcattgaaa taacagaaaa tatattaata gaaaatacaa atacaattat tcaacaacta
     1381 gctgaacttc gttcttatgg tttttctgta tcaatggacg attttggttc tggatattca
     1441 tcttttggta tgttgcaaaa tctacctttt gatataatca aaattgatag gatattttta
     1501 tctcataatt taaatgattt taaaaataca accattttgt actctataat aaacattgca
     1561 agagctcttg ggatgcttac agtatgtgaa ggtgttgaaa cagaacaaca tgtagaattt
     1621 attaaaagta taaattgtga tatcgcccaa ggttattatt atgcaaaacc catggagtgc
     1681 aaagaatttg aaaaattact atctaaagag aataattcag agaaaatgtt tataaaatat
     1741 tctgatatag gaaaatcttt aatagagaag atatttgatg gattttttat tatgcaagat
     1801 atgaaaaaac taagagctaa tgtcactgaa gacattcaat ggtttgattt ttacaacaaa
     1861 actgaaatta catcttttcc taaagtaata gagcattttg aaaaacatat taaaggtaaa
     1921 aaattttcta ttctttacaa aagtataacc ccacataata ctgaatcaga ttcattatta
     1981 attagtggag aagccgtttt atcagatgaa gcttctgaac atcaaaaata tagcaatttc
     2041 tactttagtg caaattgtat aatttcagaa aataaattac tcttagcaaa attacatttg
     2101 gatttaatta aaactgctgg ctatataaat gaatttgaat catattcaat acctaaaatg
     2161 acaaatacac atcttgatga aagtaatatt ttatcacaat tttattcaat gttgcctgta
     2221 ggtataattc gctacgattt atttggtgat atgattatta cttatatgaa tgaagaaatg
     2281 tttgatatta taggttatac aaaagaacag ttttttggtg agatgggcgg aaatttaaga
     2341 gcaattgttc atccagatga tttagacttt gtttataaaa aaagtttgga tatgattgac
     2401 acaaataaaa ttgaaccatt tttatataga ttcataaaac gagatggtca aattgtcaca
     2461 gttatgtata cacaatgtaa cctttctgca attgatggaa ggtcgattgc ccagggtatg
     2521 tatgtagatg taaataaaat aaaaaattta acaaaaatct agtacaaagt attttatttt
     2581 ggagaaatta atatgtttaa agaaattttt ttaagaactt ttccaggatt tctaattata
     2641 agagattcta actacagaat tatatttata aatgataatt taaaaaattt aattaaaagt
     2701 tatatgcaag ataatccact tggaatgact aatatagaaa tagctaagaa actccctgat
     2761 aatattgcta aattttttac ggattctcac aatattcaat tagattggga aaaaaattat
     2821 ccttatgata aaatttcaaa ttggatttta gagtttaaaa aagatactac aagctattgg
     2881 aatgttttag aatataaagt agatgttgat gaaaaaacct acataattac tatggccaat
     2941 gatataacaa aactctatga agaaaataag agaaatcttc actactcaat tactgaccca
     3001 ttaactggag catataatag aaaatattta aatgatagat ttgacatatt tataggagat
     3061 tatattgtac taattgactt agataatttc aaaatgataa atgactatga aggtcataat
     3121 gtaggagaca agattttatg tgattttgtt tcacttttaa ataaagaatt aataaattct
     3181 acttctatta taagattagg aggagatgaa tttattgtta tattctctag tgatgttgac
     3241 aaaaattatg tttattcaca actagaagcc ttaagagaaa attttttaaa agtattctct
     3301 aagtataagt atctttcatt tagttatggt gttgatacag taaaaagaaa tttaaaattg
     3361 acaatagttg aattggataa aaaaatgtat aaaaacaaga aaaaatttga taaaaatgat
     3421 tattaatatt gttattttaa aataaattaa aattatcttt ctttttttaa acttgtacat
     3481 tagtttataa atatgtatct atacaagata ttgacattaa gtactgaagc atatattgtt
     3541 tcagttcttt tttgttaaaa ttaacttatt taacaaagat atcatagttt aacaaagctt
     3601 ttaatttaaa tatattgatg tataatataa ttaaaatata atagcaaaaa gaaaggagga
     3661 atatcatgag catactccta aaaaaagcac ccaaattagc aaaacatatt ataacaagtt
     3721 tttatataaa tcgggatatt gatgaagtat taaaatacct ttgtgaaaat gttacatgga
     3781 tcggaccagg agaacaggaa tttctcactt cttttaatga aataaaaaat tatttttatg
     3841 caggtcaaga tgaaattcct tcttgtgata taaataatga tatatttgaa actgtaagtg
     3901 aagatgagaa tagatgtatg gtattaggaa gatatactgt tcgaactaaa gaaaatactc
     3961 aaatgatttt agaggtaaat caacgttgta catttgagat aattgaggat agaggaaaat
     4021 tattagtaaa gcacatgcac atatcaaacc cttatggtga aatgcaactt gatgaatact
     4081 ttcccactaa aattggaact caaagttatg attatttgca aagattacta aaggaaaaaa
     4141 cagaagttat tgaaatgatt acaaataata tcaatggtgg cttaaaaggt agcaatgatg
     4201 ataatacata ctcatttttc tatgtcaatg aaggattgcc caaaatatta ggatacacat
     4261 atgatgagtt catggaaatg agtggtggtt cagcagtggg tgctgtatat ccaccagact
     4321 tacctaaagc actagaagat tgtgaacgat gttttgcaaa aggacctaca tattcatctg
     4381 aatatagaat ccgaaaaaaa gatggaacat taatgtgggt attagattct ggaatgaaat
     4441 cattaaatag tgatggcata gtaaaaatta atagtattat tactgacata acccaactaa
     4501 aaaacataga atctgaacta aaattagaaa gagagcgata tcgtattgct ttgcaaaata
     4561 ttacagatat catgtttgag tatgatattg aaaacgatac ccttataaaa tatcaacgtg
     4621 tagaaattga caaaaaaatt gaattagaaa attttgaaac aaaaaattac tccaaactat
     4681 tagaatctgg taaaattatt caccttgatg atattggaaa actactagaa gtacttcatg
     4741 gaaatcttcg tgaaacaatt gagataagac aagtaaattc attaactaaa aatgaatgga
     4801 gatggattcg tgttcaatgt tcagttatat atgatagtga ccataaccct atcaaaacaa
     4861 taggcgtttt aaaagacata actgaagata aatctaaatt agaaagctta attaatcagg
     4921 ctcaacgtga cccactaaca caattatata atcaaagagt atcacaaaat cttattgaag
     4981 agtatctatg tagttctgat tccaaaaata atgatgctct attagttatt gatattgatg
     5041 attttaaaac tgtaaatgat acttttgggc atttagaggg aaatgaagtt ttagttgctg
     5101 tatcaaaaat actattacat aacacacatg ataaagacat agttgctcga attggtggag
     5161 atgaatttac aatatttata aaatcattaa caaaggattt aataataaaa attactaatg
     5221 atattttaaa tgatgcaagt aaaataaaag taaaggacaa tcataaaata acattaagta
     5281 ttggtattgc cttcactgat gattcaacta aattatataa agatttattt tctaaagctg
     5341 ataaagctct ttatctatca aaagcagatg gcaaaaattg ttatagtaca tacgaataat
     5401 aaagctattt atacgaccaa gaatgaacct ttaaatttaa tttcatagtc taacaatcaa
     5461 agagttgaag ctaatcttat agctagtcaa ctagttccta gaaaaaattt aaacaatata
     5521 tttattgata ctgctcattt ttctaaagag caaatttata taaaaaatta aacttttatg
     5581 tacatacaga tactaatgat aaacagttat cattagtatc ttctatctta aaacaaaatg
     5641 ataatttatc tatataaaaa agtgctttgt tgacagagtt cactttttta tataggtaca
     5701 gaatttaagt ataaatacat gttttattct atacatttta gttaatgtaa aatttcttga
     5761 ttgtactctc taacttttca acattatact atttatatac cacttagtta atataaaccc
     5821 cttagctggt tctagtgctt tttctatgat tttagtcata tttacatacc acttagttaa
     5881 tataaaacca atttagatga tgtatatgtg caaaaggaag aaataattta cataccactt
     5941 agttaatata aaacgctact aaacttgata tacatgacca ttataataat aaatttacat
     6001 accacttagt taatataaaa cttccataaa aacatcccct ttttattctt cttccttata
     6061 tttacatacc acttagttaa tataaaacag tcttttttta atgcagagat gcaagaagtt
     6121 ttataattta cataccactt acttaatata aaactatcag tatttaagta ataatttaag
     6181 taatatagga tttacatacc acttagttaa tataaaacgg tcactacaac ttatacttcc
     6241 agtctaacga tttgaattta cataccactt agttaatata aaacatattt tggatttatt
     6301 aatataaaca taattaaaaa atttacatac cacttagtta atataaaacc ccaaaataaa
     6361 ctgggcattt tcaatactta cacatatgca actctcttaa atttgcagtg aaccatgagt
     6421 agtgcaaatg ataaagctta tcatataccc tcaacacctt atatttcaag cgttaagctc
     6481 tactttgtaa caaatatcgc tcactgcaaa actatttaca tttttattat atcataaata
     6541 tattattttt gaatatctat accaatttgt ggtataataa aagcaagaag aactacaatc
     6601 tatttagacg tagagtgaag ttcaaatact aatgtttatt ctttttgaac ttgattttta
     6661 gttcaaaact aaagtcactc ctgcctgtgt gaactatacc cgctgtttta tacagttaaa
     6721 acatgtaaaa atagttataa agtactagtg taactcatta gtactctttt tattatattc
     6781 atataattaa tatctatatt ttaaagttta ctatctaaaa aagagcacgt caaataaaac
     6841 cctagatatt taattttttt agagatactt aaaataaatt atgctctctc tgtacttaaa
     6901 tatttcctaa attcaaatgg agacatagaa tttaatctat tttgatatct aaaattatta
     6961 tagtagtata tataatcatc tattgcttta actaattatt catatgtatc aaatttcctt
     7021 aaatagtaca tttctacctt taaaattccc caaaatcctt ccataggagc attgtcaagg
     7081 caacgaccaa ctcttgtata atatatgtat atctaacacc attaagaaat atatatttta
     7141 taatatatat ttcttcattt aatgcactta gaaaagcaaa gaaaatacag attagtacac
     7201 tttttagaga aaaataaaat taatacatct aaaatattaa aaaatacttt agataatcta
     7261 aagacacatc tcaaagtttt taactaaatt ataaaactaa ttacaaatgc tatgtaacga
     7321 taataaataa aaaatttact tcgcttaagc gacgtgtcgt tgaaatattt catgttgcca
     7381 actggttcgt aagttgataa gaaaagaaca ctaattattt agagttcttt tcttagctac
     7441 aagtggatta taatactttt aaatctgtga gaaaatcata tataataatg atatgaacaa
     7501 gtaaagaaaa tacaaaaatg acaaaattat aaataaaata ttataaaatc ataaggtcat
     7561 aattgttata ttgtagaaac aaagtaaaga gagagggtgg agaataaatg aatcaaaaaa
     7621 aaataaagat tagtattaaa atggtaatta taggtatatt tgctgtaatt attgcttttg
     7681 tattatccag attattcgtt gagtatattg atgaattatt aaggtctaat tggcaatatc
     7741 agctatttga aagtgagagt tcatatatag tatttgccaa ttgtattatg ttctcgaata
     7801 tattattaga aatattttta atatatattt gtagaaaatt tagaaaaatc taatatggtc
     7861 aacgtatttc gaatcgaata cttcatgatt atttagtcta ttgtgctagt taaagtttaa
     7921 ttcccatata aagtaaattg attttagtaa aacaaatcac ccctaagggt tagtttatgg
     7981 ataagtcatc caaatattaa ctctttaatt tttgctatag attcatcttt gctcataact
     8041 ttacttataa aaaatgaaat attataagct agaactttta ttctaattct agtcataaat
     8101 cctagcatag agttactttt tttatattat aaataaaact atacaacaaa gataatattt
     8161 agtaaaaata actttcattt taactaaact taaaattcaa ttactctcta aattgacgtt
     8221 taggaattag ttcaaaataa aaaagagtgt atatattgaa attactacat ttcaatatat
     8281 acactctttt taaatttctc agttttccat ggaactttac tttatttatt tctgtattag
     8341 ttgtattttt tatgcatttt tctttaaaat catattttct gattcaactc ttatattata
     8401 aaatttcttt actaaattat tctctttttc tatatcttca tatatatttt ttaatcgcct
     8461 tttacctaat cttttatcta acattactaa tgatttaact aatatattat atgattttat
     8521 cgcatcttct attgagatat ttaaacatat atttactgct tcataaaaat caaattcatt
     8581 aaaaaatcca tttttattta caatcttata tccctttgca tctctttcta aatactcttc
     8641 ctaaatactc ttcaaaactt tcatcatcta atctaaatcc tatatcacta tgaaaaatag
     8701 gttttgcatt tggatatagt tctattgttt tatcaaaagt ttcgaatacc aagttattat
     8761 tattcgattt tccaacagtc cactataaac aataataagt ttgttgctcc aaataatgta
     8821 acacttgtag aaagatgtta cagatatata aaagatagag aatgtagttt ttctcctaat
     8881 actattagaa atagaaaaaa tatgattaaa aatcaaattg aagttttctt taaagatatg
     8941 aaattaatag atataactcc ttctatttta cagtcttata taaataacat atacaatgag
     9001 cacatgttaa attcaacaaa aaaccaagtc gattttataa aatcagtatt gaaagaatct
     9061 tatagattaa aagaaatatc agaaaatatt tgtgattttg ttacaacccc aatcaaaaaa
     9121 aatagttcaa cttctaagct ttatacaaaa caaaaagctc aattattatt agaaaaatca
     9181 aaaaatattc ctataggtat accaattttt ttgatgctaa ctcttggatt acgttttggt
     9241 gaagctgtat cacttatatg gtcagatgta gaccttgata aaaaatctca catgtaaatc
     9301 aaacattagt ttatgtatac aataaaataa tcttcaaagg ccctaaaacc cctagattca
     9361 aaagaagatt atttgttcct gatgaactta taagtttgtt taaaggggaa aaattaaaac
     9421 aaaataagtt aaaattacag ggtatattga aaaatgaatt tgatttgata tgcttaaata
     9481 aaagattcaa cccgtgagtt caaccaactt ttgttgttca ttttaaaaaa ctattaagag
     9541 ataataatcc tagagatata aaagtacatg aattaataca tgcaaatgcg actttgatat
     9601 tactttcagg tactaacata aaagctattt ataaaatatt agtacatgca gatataaaaa
     9661 tagctatgaa tatatattcc atattctcac ttattagatg aaatgaataa agaaacatct
     9721 gaaaatataa gcaaaatact attcaaataa tgttcagttt tatgtcaatt aaatgtcact
     9781 caaagtgctt tttttccatt tgtcagttaa atgtcactta aagtaccttt ttcctatttg
     9841 tcagttaaat gtcagtaata tataaataag atatatttat aagaccctta aaatcactat
     9901 tatttaataa aatttataat tatatactat ttgctgttga cattggtatt ataccatagt
     9961 ttatcattta tattagaata aaattattaa ttaatagttt cgtaatttta taatagattt
    10021 acagggagtg ataataaatg gatacaaaaa tatctataaa cgaaaacatg agtctaggta
    10081 aaagattttt taaataccta gcaccatctg tagttgcaat gtgggttttc tcactatata
    10141 caatggttga tggaatattt gtaagtaaag gagttggaga attagcttta gctgctgtca
    10201 atatatcaat gccttttatt aattttatat ttgcagtttc tctattattt tcaactggtg
    10261 catctaccat aattgcaatc tatctgggga aaaaagatat taaatctgct aacgaagtct
    10321 tttcatttaa cttagtatca ataattatat tatcaataat tatattagct ataacttttt
    10381 ttaatttaga tagattagca ctatttttag gagctacaga atctacaatt ggtatggtta
    10441 aagattattt aggaataata atatttttta acggattctt tatagtgtct tattctttag
    10501 aagttataat aaaaactgat ggctttccaa tattagctac tgttggtgta ataatatctg
    10561 ctcttacaaa catcatactt gattacttgt ttgtaattga atttggatgg ggtgtaaaag
    10621 gtgctggtat tgcaactggt ttgtctcaag tcttttctac tattttcttc ttaatacatt
    10681 ttttaagaaa aaactctact ttgaattttt caaaatttag aattgatttt aagacactta
    10741 gaaaaattgt atttataggt tttcctgatt caactactga attaagttgt ggtatagttg
    10801 ttttactttt taacttaagt cttactaaat atattggaga aaatgctctt atttattata
    10861 gtgtcataaa ttatataaat acattagtac ttatgactat gatgggtata actcaaggta
    10921 tgcaaccact tactagcttt tattatggtg caggaaatat agataatgta aaaaaattat
    10981 taaaaatggg gataaaagcc acaataatag catcagttgc tgtatttgca atttgtatgg
    11041 ctttttcagg accaatagtc tctttattta tacatccaga agaaacaatg ctattcaatg
    11101 aaggagttcg tgtatttaaa atattctcta tatctttctt attggtagga attaatgtaa
    11161 taatttctgg attctttgta tcagtagaaa aaccatcaat ttctactgtt atatctcttg
    11221 gtagagggct tgtaatagtt gttttaagtc taatttcaat gatactaata tttggtggac
    11281 aaggaatatg gatgactact attgtttctg agtttatatg tcttatttta tcattagttt
    11341 ttctaaaaaa gaatttttct acattagact ctaatctaaa taaggttgct tagatgataa
    11401 gagtgacaat atatgaaaat atctaaataa aaaataccta ttatacaatc ttgcaaatat
    11461 taaacctttg caaaaattag gattatataa taggtatttt atttatgtaa ttattttatt
    11521 tctaaatatc catagccatc ttttattaaa gttcctttta cttcatattc tacaggtttt
    11581 ttaaatattg gaccatctac gacaaatgta tgatactctc catgttctcc acatgggtct
    11641 gcaccagcat tttttatctt ctccacaagt tctctagtta atttttttcc taaaaattcc
    11701 tctcctaagt gctttaagtt tacttttgtt acaacactac aaaatccact gtctataaat
    11761 tcatatacta aatcctctct actctcttgc cataatggta aatctacttt cataccaact
    11821 gcttcacatc tatcagtatc ccattttcta tgactttcta tatctatatc tccatatgca
    11881 catatcgttg ctcccatgtt tttagctttt attaaagccc tctcaaagct ctcttcatat
    11941 ccttctacgc cacactcagc aactaataaa ggtaatccta tgttgctact tacttgctta
    12001 agcatattat attctaaatt atgtgtccat gactttccat gttcttcact tatagttgtc
    12061 aagagtgcca caggcttata tccttttttc aacattcgat ttaatgctaa tatactatcc
    12121 ttaccaccac taaatgacat tacaaatttt tcatcagcca attttaacac ctctttcatc
    12181 taactttgtt ttattaacat aattatacca aatttttgag ttgcatagca aattatatta
    12241 aattgttaat tcactataaa gaatgtccct aatattaaaa gtataacccc tatgtatttg
    12301 tataaatgca ttgtttcttg tcccaaaatt acgctatcaa taaaaaatcc aactaaaact
    12361 tgggatacaa gagttaatat aagtgtattt gatactccta aatttggaat tgcctttact
    12421 gtaaaatata ttattagacc tccaaatact cctcctaata acaacttata attaaaacca
    12481 tttatagata ctagacactt taagtcacct gtaactatta tacttgccaa aaaaaatatt
    12541 gctcctacag ttaagcttat aaaggtggcc actaatgctg ttgtactttt tcctagctct
    12601 ccatttataa aagcttcaag agctgtagaa ccacctgcta ataatgcaaa tattattgcc
    12661 cataattttg tcatggacaa acctccttct atataattct attaatagaa gtttatgata
    12721 caacctatta attaattact aagtatagtt taatttttat acactaggct ttatttggat
    12781 atgaatttct gttataattg ttctatcgat tttggaggtg attttatgta cacaaatttt
    12841 aatatcgatt ttaataactt taataaaaaa gaaaatgcta ttaaatttat gttaatggga
    12901 gtattactca ttatattagg attattatgc cttacattta agacattagg tataaaatta
    12961 atttcttgga cttttggtat tgcactttta ttctttgcat atttaaattt aaaaaatatt
    13021 aatgagttaa aaagatatgc tactaaagaa gaaattaaac cctctattaa tatccaatgg
    13081 gttctaataa ttgcatgtat attacttttt gtatttcctc aaaaaataca aagcatattt
    13141 tcattatttt taggatttta tttaatcttt aatcaactag tagcacttgt aaatagtaag
    13201 aataatcctt attctaaatt tacaacttgg aatatagtta agattctatt tggtatatgc
    13261 cttatattat ctccactttt tttatctaga tttatagtat cgataatgtc ttttttcata
    13321 atattatttg gactagttct atttttctct ggaaatacag cgagaaaata ttaattattt
    13381 tctcgctgta tttttatatt acttaaaata gaatttgttt aagttatcta tttgtggtaa
    13441 tatgtttttt gtacaagctc agcgtacaat tttattagaa ataggtgatt ttttggataa
    13501 atttaaatat gcttttgata ataaaagata tcatacttgg aattactatc ttagaaatac
    13561 ttttggagaa aaagttttta aggtatctat aaatgcagga tttacttgcc caaacattga
    13621 tggaagtctt ggttatggcg gatgtaccta ttgtagcaaa gaaggctcag gtgattttgc
    13681 tggaaaccct aaagataatc ttatcagtca attttataat ataaaagaaa tgatgttaaa
    13741 aaaatggcct catgcaaagt atattggcta ctttcaagcc tatacaaata cgtatgctcc
    13801 tcttgaagtt ttaaaagaaa aatatgagac tatattagaa cttgaagatg tcataggtct
    13861 ttcaatatcc acaagacctg actgtttgcc agatgatgtt gtagaatact tatctgaact
    13921 aaataaaaga accaatttat gggttgaact tggacttcaa acaattcatg attctacatc
    13981 taaaattata aatagaggtc atgattataa aacatttgtt gaaggtgttg aaaaactaaa
    14041 gtctaaaaat ataaaggttg tcgtacacat tataaatggt cttcctggtg aagattataa
    14101 tatgatgatg gaaacagcaa aggcagttgg taaaatgggt gtagacggta taaaaatcca
    14161 tctattgcat gttataaaag atactcctat ggaaaagatg ttacaaaatg gtatgttaac
    14221 tcttatggaa caagatgaat atatcaatct tgtctgtgac caattagaaa tacttcctga
    14281 aactatgata atccatagac tcactggtga tggtaagagg gatgaactag taggtcctat
    14341 atggagccta aaaaaatggg aaattttaaa tcaaattgat gatactttaa aggctagaaa
    14401 ttcttatcag ggctgtaagt ttgtttaata caaaataata actatagaaa tggttatata
    14461 aatatagtcc tttctatagt tatttagtat ttacaaaaca aaatattaat ttgtacattt
    14521 atacaatata taataaaaaa tagatagtct aaactgtatc tacttatatt ttatttgtac
    14581 tgtccacttt attctataaa atttataaat agttattttt taataccaaa tataatttgg
    14641 tataattttt gttggactct aaaatgtata ccaataaatc ctttttaagt atataattct
    14701 aaatactttc agttttttca atctgcttta acttgttgga cttataaaat attcttattt
    14761 catcataaga aagattaagt gctactactg cagatattaa catcaacatc actccaaata
    14821 acttccctaa ggaaaaactt tctcctaata tagtgcaacc aattattact gaaccaacta
    14881 attctactga tgccacaaca cctgcaactg acaactctac acctttagat atacctgctg
    14941 cataaaaaat atatgcaagt gcagctggaa caatgcctaa cattagcata caagataaaa
    15001 catctaaatc cttagcataa tttaagattt cccaaggtct tgaagaagga atcataaata
    15061 tagctccaaa taagaaactg tatactaata ttgttgaact tgaaaattcc tttaaagcat
    15121 ttttactaat agttggcata agtgcatatg ctattgctga taatacccca agtaaaagtc
    15181 caaaagcgtt taaaccttga aaatccaaac gtccaccagt cactgccata attgcaccaa
    15241 taaaacaaag aataagtgaa aataatttgc ttctagtaat attttcttta taacatattt
    15301 ttgaaaatat tgctaaaaac agtggtgatg tatatagtaa aactgctgca attgatacac
    15361 ccgcaatgtc aattgcttta aaataacata aattaaacat tgcctgacat ataataccta
    15421 ttattactga atatataatt ccttttttgc ttatctttaa gatttgtggt gtccttatag
    15481 atgaatatac gcctaaaact ataaatccaa aaaacaatct tgtaaatgct acttgttctg
    15541 atgtcagtcc atttcccatt aaaaactttc caaataatcc aagtgtagcc cataataacc
    15601 ctgctattgc tataaataca taccctttac ttttttctcc cattttaaat tcccttctat
    15661 ttttatttag tcaatttaca ctcttctttt taagcaaaaa ctatgccatc ttaaatcaat
    15721 atatatcgac tctaaaaccc tagtctttcc aataatacta ataaattttc aattttttta
    15781 tatttatttt tcacttactt taagttgatt tagacttatt ttaattgtaa aatgagttat
    15841 atataactta tttaaagtta aaatagactg aaacttatat ataaatatca aattttaagt
    15901 atattaatca attaaaccta ttttaatata tcattaaaaa tttaggagga aaatataaaa
    15961 atgaatttag atttggaatt ttatcaaaaa atattagaag cttcccatga tgaaatatgt
    16021 gtttctgatg ataaaggaat aataatatat tgtaataaag catttgaaga aaattatggt
    16081 ctaaaaaaag aagatatttt aggaaaaaat gtttcttttt tagaagactc tggatattca
    16141 acaaaaagcc ccataccaat tgtattaaaa acaaagacta aattttcact ggaacaagat
    16201 actcaaacag ggaaaaagct tatcataact gctaccccta tatttgatga aaatggaaac
    16261 ttagaattta ctgttgaaaa ttgtagagat ataactgaac ttaataatat aaaaaataaa
    16321 cttgaagaca caaaaaagca agtaaaaaaa tataaatctg aagtagaaac actatacaga
    16381 actgcactta gaattgagga tactgtaatt atggacggaa tagttatgag accaatcata
    16441 aacacagtaa atcatgtttc taaaacggat gtaagtgtac ttttattggg tgaatctgga
    16501 acaggaaaaa gctcacttgc tagatatata caccacaaca gcaatagatc taatggccca
    16561 tttataacaa taaattgtgc aactatatct ccacaacttc ttgaatctga actttttgga
    16621 tatacttcag gtgcttttac aggtgcaagt actaaaggta aagttgggct cgtagaatta
    16681 gctaatggag gaactctttt tttagatgaa ataggagata taccacaaaa tctacaggca
    16741 aaatttttgc aattaataca ggatagaact tttactccag ttgggagctt aaaaaataaa
    16801 aaggtcgata taagaattat atctgctaca aatgttgatt tagtttcaaa agtaaaagaa
    16861 aaaaagttta gagaagattt atactataga ttaaatgtta tagaaataaa attgcctcct
    16921 cttagagaaa gaagggataa tctagtggaa attataaaat attattttaa taggtactca
    16981 agtgacttca atctaaataa aactatttct aaagaagcta tggatgctat tgcaaattat
    17041 agattcccag gcaatataag agaattacaa aatataattc aaaaaatctt gcttacttgt
    17101 acagataatc atataacaat agatgactta ccaaacattc ttacaaaaaa tatacatatt
    17161 accaataatg gaaataaaac acatatatcc caaattaata aggttattat atctgactca
    17221 aaatctacta attataaaaa taaaaatttt gacactttaa ttaaagagta tgaaaaaaat
    17281 ataattttgg atgcttatga aaaatttggt agttcttata aagtagccaa acaccttgaa
    17341 atctcacagt ctaaagccaa taggttaatt agaaagtata ctaatactta attttaaatt
    17401 tgataaataa aaacttaaca ctatttacat attgtaaatt tggccattta ctgttattat
    17461 gaatatggtt gaaattattt aaataaatac tgttgactta gtttcatata tttaccatta
    17521 aacttttatc ataaatatca tatcataata atatatttaa taagtctaat aaactatacg
    17581 aggtgtaaat atgttaatga atacccattt tattatggca aaatcaatac ttgataatat
    17641 agatgaaaat aaaacattct ttataagcga aaaaaatttt atatatggca atataaagcc
    17701 tgatgctttt tctaaatata aattaaaaaa acattattta gatgagtcat ttaatatgat
    17761 tgtttctaaa ataaactatc tatgcagtct taatatagat agtctttcta aatacttttc
    17821 agtaagtaga cttagtcagg aactaggagt catatgtcat tttttatgtg attttttctg
    17881 tgtagctcac agtgaacgtt gggagtttaa gcatagctta aataaacatg ttacttatga
    17941 aagagaactt ggagcatttg caaaagatgt tgatttatct aaaattaaat gtggtactat
    18001 aaatagtagt tttgagaagt tttttactaa actttatagc gattacaaaa agaaaactga
    18061 ttatatgaat gatttaaaat tttcagctta tacttgtaat agtgtcataa attatatact
    18121 tgactctatt ttatctaata caataaaatc atatcatata gcaaattgcg gttaacttaa
    18181 gaatttttaa acttatataa acttataatt gaatataatt aaaatgactg ctctatagaa
    18241 tataatttta tacttctcta ggacagtcat tttttatact aactatttat ctccattata
    18301 acatctatgg cactttttac actatctact acataatttg cactttgctt tacatcttcc
    18361 cttgccttag acattgcaaa actatattta aatccttcta acatgcaaat atcatttgga
    18421 gaatccccaa tagtagcaac ttcatctgga cttacattta acgtttttat tacatgctct
    18481 atacttcctc tttttgacac acttttagga actatattta aagtatatgt tccagaaaaa
    18541 tatatttcta agacttcact aaactcactt tttagataaa gttctattct ggataactct
    18601 tccaaatttt cagaacatac gcctatattt cctataagat tatcaaaact acttcttttt
    18661 tcaaagtctg ggtcaactat aaagtcacta gaataatgtc taaaagcagt ggtatcacta
    18721 cccacaatta catcaccctt atatttaaaa taaagttgtg catcacttga ctcaaatcta
    18781 tcaatgactt tttttactaa attatcatct atcgttcttt taaaaattac attataatct
    18841 ttatcatata taaatgaacc attttcacat acatagtatc cattcatatc taaactatct
    18901 ttgataatat tatgtgcttg tttaaaaatt ctacctgtag caacattaaa ctgtactccc
    18961 tttttttcta tttcttcaat tgctactata tcttcttttg ttatggttcc attttcatat
    19021 agagttccat ctaaatcaca aaatatatgt tttatcataa attaaaagcc tccaaaaaca
    19081 attttatatt ttactaatac tttaatcaaa ataacttaaa gcttttctaa ttgtattctc
    19141 aactcattaa gcaaatctct agtagctctt aatcttatct ttcttctatc cccattaaat
    19201 atatttttct ttactattgt ttttccattt atatatattg cagtataaac cagtcccaca
    19261 ggcttctctt tggttcctcc accaggtcca gctactccag ttgtagatat acctatatta
    19321 gctttaagtc ctttagcaac gccctcagcc atttctatag ctgtttgctc acttactgct
    19381 ccaaactctt ccaaagtttc tctttttact cctaatcttt tcattttaga atcattagaa
    19441 tatgttacac aaccttcaag aaatacagag gatatacctg gatagtttat aagagatgaa
    19501 gaaaccaaac ctccagtaca agattcagcc actgctattg ttagattttt ttctacaagt
    19561 agcttagaaa cagcatcttc taaagcagtc tccccttcag atatggaaat atctccttca
    19621 gtatatacat atttaccaac tctacttttt attttttctt ctactggttt tattaattta
    19681 aaagcttctc tttcattttc agcctttgca gttattctta ttgtaacttc taattctttt
    19741 gcatataaag caactgttgg attagtctgc tcattaatta tatcgagaat ttctaattct
    19801 agatttgact cacctattcc atataatctt aaagtcttag atactagcat ttcatttgtg
    19861 aattgttgta agtaaggttt gacactctca ttaaacatag ctttcatttc tcttggaggt
    19921 ccaggcaata ctattataaa tttaccatct ttttttaaaa ttgcccctgg agctgtacca
    19981 ttattatttt ttagtactat agcatctttt ggaaagtaca cttgtttcat attattttcg
    20041 gccatttttt tccctgtttt aacaaagaaa gattccaaaa cctctagtga aggtttatgt
    20101 agttctaatt tctgaccaaa gtattctgca gcagtttctt ttgtcatatc atcaccagtt
    20161 ggcccaagac cacctgtagt aattacaaaa tcacttctct ttaagctttc atcaaaacat
    20221 tctaaaagtc tttgtttgtt atctccaaca gtactctgat ggtatacttc tattcccaat
    20281 gatgcaagtt ctttagctaa aaattgagaa tttgtattta ctatatctcc caataatatt
    20341 tctgttccta cacttattat ttcagctttc aaaatttatc actccttgat ttatagccat
    20401 taagactagt cttaagttca taacaataat tttagcaaaa ataataacat aaataaagtt
    20461 aaaaaatcat ttaaacatat ctttaaacta gattattatt ctaaaaccac acataatata
    20521 aacttcgtat atagtggtac tttcatcctc aactgttaat tcatataatt aagtatgtaa
    20581 ataaaaagtt tactttaaaa taataaaaat taaaatagga gtgtataata tgaaaatagc
    20641 agttataatg ggtggaatat catcagagag agaagtttct ttaaattcag gaaaagagat
    20701 atataataac cttgataaaa ataaatatga agttgtaaaa gtaataatag atgataaaaa
    20761 agatatattt acaaagatac cagaagatat agattttgca atacttgctt tacatggaaa
    20821 atttggtgaa gatggatgta tacaatcaat acttgagact atggatatac catactctgg
    20881 atgtggacct ctttgcagtg ggatgtgtat ggataaaaat ataactaaaa aaatgcttag
    20941 agattcaaac ctacctactg ctccatgggt tttagtaaaa tcagttgatg aaatagacta
    21001 tgatgaaata gataatatag gttatccagt atttataaaa ccaaactcag gaggttcaag
    21061 tgtagccaca ttctttattc attctaagga tgaagttgaa gaagcagtta gaaaaggtct
    21121 ggaagttgat gaatttgtaa tgattgaaaa atatatacca ggtggagaat atacttcatt
    21181 catattaaat ggagaagtgt tccctacaat atctataaaa tctgattcag gattctttga
    21241 ttatgaagct aagtactcag ttgaaaaagg tgcaaaggaa gaagttgttt acttagatga
    21301 agaacttcaa aaaagagtaa acgaaatttc tgagacttgt tggaaaattt ttaattgcaa
    21361 agcatatgta agagttgata tgattataag tgaaggtatt ccatatgttc ttgaactaaa
    21421 tactcttcct ggaatgactc aaactagttt aattcctaga agtgctgctg ctagaggtat
    21481 taagtacagt gaactattag ataaacttat agaatactct ttaaattaga tgaaataatt
    21541 tgaggtggta atttgatatt ttcaaatcta aaactaaatg ataatgaacc aatttatata
    21601 caacttaaaa attatataag tgatatgata tcaaaaggac taattccaga caatagtaag
    21661 ctcccttcta caagggagct tagtcagctt ttacaagtta gccgaaattc tgtagtttta
    21721 acttatgaag aattaaaatc agaaggatta atctactcta tttcaggaaa aggtacattt
    21781 gttaaatcaa agaataagtc ttcaaatact acatggtcat taaactggga ttgtttagag
    21841 aatacatact ctaaaaaagc caatgaattg gacattataa aaagtgaaat tccttggagc
    21901 tctgacctga tttcctttaa aagtatttct ccagatggag atttattcga catagaagaa
    21961 ttaaaaaaat cctttttaaa tagaatttca ctagaaggac ataaattact caactatgga
    22021 tatgcacaag gttataagcc ccttattgac tatttactag aatatatgac aaataagggt
    22081 gctgatataa gtaataaaga tatactaata acaaatggat tcacagaagg ctttgatatt
    22141 attatgtcat cttttaccca agaaaaagac tacataatat gtgaaaatcc tactcataat
    22201 actgctataa aaattatgaa atctcataac ctcaatatct taggtgtaga tataaatgaa
    22261 gatggtttgg acttcaatat gctagaagac aatttagtta agtataaaaa taaaataaag
    22321 tttgcataca ttacaccctc ttatcacaat cctacaggaa ttgtaatgac tcctgaaaat
    22381 aggtacaaat tctataatct tatgaaaaaa tacaatatag caattataga agatggattt
    22441 aatgaagaat tactctacaa tagttctcat atatttccta tctgttccct agataatatg
    22501 aataatgggg tcgtatatat tggtagtttt tcaaaaattt tatttcctgg aatgagaatt
    22561 ggatggatat ttgcagataa aaaagtaatc aataagctag aaagtgtaaa aaggtgcaga
    22621 aacatacatg tatccttttt ggaccaagga attttatttg attatttaag taatggaggt
    22681 tttgaaaaat acattaaaaa aatacgtaaa ttctatgggg acaaatttaa ctttgcatat
    22741 aagtgtataa aaaaatatat aaaaacagaa tatatcttgg gtgatggtgg tttgcatata
    22801 tttataaaac taaaaaatat agatactaga gttttattag aaaaatgtta tgaaaaaaat
    22861 gttatattta tgcctggaga tttatttttt acagataact ctggttttga tactttaaga
    22921 ctcggatttt ctaggttgtc ttttgaagac attgaaattg gaataaaaat aattggagaa
    22981 actataaatg aaatgttcta ggttaactaa atattgttag cctagtttat ttttatgagg
    23041 tataatatat atagtttata tgaatctaat actgggggat aatatgagta aaaagtcaaa
    23101 acgaagaaaa attaataaat taaaattaac gattgtaata actgtattat ttcttgcttt
    23161 attatctata tatgagatac tgttctctaa tcccaacaaa aaaggtaata taaagagtca
    23221 agaaactatg caaaataaaa ataatacaac agatgattct aaaaataatc aaagctctac
    23281 caaaaagcag gaagataata atatcaagcc tactttttca tattcttcaa taccagataa
    23341 tataaaaaat aaaatgattg gtaaatctat gcctactgat gaacctataa gctttgatag
    23401 tctatcttat ttaaaactta catattatgg atttgatgaa aaaactcatc aaggagaaat
    23461 gatagtaaac agtgaactag ctccagaagt agtggatatt tttaaggaat tatacgaaaa
    23521 aaaatatcca attgaaaaaa ttaagttaat agatgactat gatgctgtag atgaaaaatc
    23581 tatgtctgat aataatacct catcattttg ttatagaact atagctggta caaatgtagt
    23641 ttcaaatcat ggtaaaggac gtgctataga tataaaccca ttacagaatc ctcaagtttc
    23701 tggaaatgat gttacaccta aagtaagtac agtttatgct gataggtcta gcacaaaatt
    23761 tggaatgata aaaaaaggtg atgattgtta taatgctttt gtaagcagag gttggagttg
    23821 gggtggctat tggaaaaacc cagattatca acattttgaa aaataataga ttagaaataa
    23881 aggtgaatta tatgaattta atgacattgg aaaatataag taaaagctat tctgaaaaaa
    23941 aattactaga aaatatatct cttggaataa atgaagggga aaaaatagga cttattggtg
    24001 taaatggtac tggtaaatct accttactca aaattattgc aggagttgaa gaaagtgaga
    24061 ctggtaatat aacaaaaact aatggtatta ggatagaata tctacctcaa aatccaactt
    24121 acaatgaaaa ttcaactgta cttgaacaag tatttaatgg aacttctgaa gaattaaagc
    24181 tacttggtga atatcaagaa atcctagaaa aacttaattc aaactttaca gaggaattaa
    24241 ataaaaaatt attatcagta cacgaaaaaa tagatgcctt aaatctttgg gatttagaaa
    24301 gtgaagctaa gtcagtactt acaaagcttg gtataaatga ctttaatcaa aaaatagcag
    24361 aactttctgg tgggcaaaga aagagagtct ctctggcatc agcccttatt acaccttgtg
    24421 agtttttgat actagatgag cctacaaacc atctagacaa tgatactata gattggcttg
    24481 aggaatatct taattccaga aaaggctcat tgcttatgat aactcatgat agatactttt
    24541 tagaccgtgt tacaaataga attattgaac tggacaaagg tagattattc agttatgatg
    24601 ggaattattc aatatttttg gaaaagaaaa tggaaagact ttcgctagaa gcaagtattg
    24661 aagataaaag acaaaattta ataagaacag agcttgcgtg ggtaagacga ggagctcagg
    24721 ctcgtagcac taaacaaaaa gctcgtttac aaagatttga tgaacttata aatagagatt
    24781 cttttaaacc tgatgaaaat cttgatatat ctgttgcatc aagtagactt ggaaataaaa
    24841 taatagaaat acacaatata tctaaatcat ttgaagaaaa tactgttata gataatttag
    24901 aatacacttt aactcgtaca gatagaattg gtataatagg caaaaatggt atgggtaaat
    24961 ctacccttat aaatatactt aatggagaaa tagagcctga tagtggatat atttcaattg
    25021 gagaaacggt taaaataggt tgtttttctc aagacgactc tcatatgaat attgatatga
    25081 gggctataga ctatgtaaaa gaagctagtg actatataga aacttcagat ggtactagga
    25141 ttacagcttc tcaaatgtgt gaaagattcc ttttcaatgg tactatgcaa tatactttga
    25201 taggtaaatt atctggtggt gaaagaagaa gactacatct tcttaaaatc cttatgacag
    25261 ctcctaatgt gcttctttta gatgaaccta caaatgattt agatattgaa acgcttaaaa
    25321 tattagaaga atacctagac gaatttaaag gtgtagtagt tacagtatca catgatagat
    25381 atttcttaga tagaatttgt aataaaatat ttgcatatga aggtaatgga aaaattcata
    25441 tctatacagg taattacagt gattatctta tttatagaga gattcaagga attgaatttg
    25501 aagaatctaa aaaggaatca gttaaaaaag atgactctac taccctaaaa aaagaaaaac
    25561 ctaaaaatga taaaaagctt aagttctcat ataatgaaca aagggagttt gaaaatattg
    25621 actctgatat agaaaaactc gaatctaaaa ttgcaacact tgatgaaagt actgcaaaat
    25681 tttcaactga ttttactaaa cttcaagaaa ttttagatga gaaatctaaa ctagaaaaag
    25741 aacttgaata caaatatgaa agatgggaat atttaaataa tctagctgat gaaatagcta
    25801 ataataagta atattaaaat aatcttaagt ttaataagtt ttgctttaaa tctgtaattt
    25861 ataaatattt taaaggaagt gtctcaaaat gaacttttta gttttatgag acactctttt
    25921 ttatgagaaa tcaaatatat tttcatcatt tcaacaatac aaaagaggct tatctctatt
    25981 ttgagacagc ctctttcata attaccttct aatttatatg ctctaatata tctataataa
    26041 ttcttattat atctatatat ccagtctttc aatataatga caattaactt caatatgatt
    26101 taaaaatact aacaaccttt cataaagtgg atgagattct tctccatatt tagcttctag
    26161 gttcttgcct atttctctaa cagtcctttg accatcaatt tgtaaaaaaa caaaactgga
    26221 atattcatct agttctattt ttttgtattt aggtattcta gcttttaact ttctaaaaaa
    26281 cttttgaatt ttatggtcct gtttttcaag tatagtaaca actctatttt cagctacttc
    26341 atattcaagt ccatcagtaa ttttaaatac aatatctaaa acatcattac tcttgctcat
    26401 tattttttac tttagataaa acaataggta atgctgtaag cacaactaaa actactaata
    26461 aagcaattgc cattgaattt gttgcagcaa atccacttgg tacctttgga gttacaactc
    26521 cagttacttg taatattata cctattaaac caatgataga accaccagca acaagaccag
    26581 aagataaact tataccatta gaaactttaa cttctttttc tttttcagtt ttagatacta
    26641 attcaacaaa taaacgaatt aatgctccaa ctaatattat agaagttgtt gcaattggta
    26701 aatagaatcc tatagctata gtcattattg gtaaatttaa tgcatataga actattgcca
    26761 taaatactcc aacaataatc ataacccaag gtaaactacc tgacataata ccagaagtta
    26821 atgttgacat taagtttgct tgtggtaatg caaattgtac attatcacca gttgctctaa
    26881 gttggtcaga aagtaataaa attactccta caacaactat aacaccaact atacttgcaa
    26941 ttgtaaagta acgttgcatt tcgtttttgc taccaccaat aatataagtt actttttgag
    27001 attgagtata acctccagct atagcaatag caacaactat aaatgaacca aataaaagta
    27061 atgatttatt agcttctaaa tcagtccatc ccattataac aaacactaat gttacaataa
    27121 ctagagaagc tatagtcata ccagaaactg gaagatttga tgttccgatt gttcctgtta
    27181 aacgaccagc tactatgaca aataataaag ataataaaag agatatgata gctccaataa
    27241 ttgccataac aatgttacca gaaattaaga atgctgctaa aaatccaata actactccac
    27301 ctaataaaag tatcatttgt atagatgaac cttcttctcc ttctccagca cttgatttag
    27361 ctttaagtgt ttcttttata gaagcgatta tagttggtat aagttttatt gcaccaataa
    27421 tacctccaca aagcatcata ccagcaccta tatattttac ataactacta gatattgctc
    27481 caacatccat ttggttaaga ggcattgcag gattattcca taccatagct ccatctttag
    27541 ccatatctgt aaagtatcct attaaaggtg ctataccaaa gtttgataaa atacttcctg
    27601 caaacattgt taaagaaact tcaagcccta ctataaatcc aattcctaaa agtagaggat
    27661 ttacttcaat atcaaatttc catttgtaga atttgcttcc tacaagactc attacattgt
    27721 ttgctacatt taagaatgaa ccagttaata cagtaataaa tccacttatt acaaatccaa
    27781 ttcccatgta cttaattgaa tctccaccag cctctgaagc aacaagtgtt tcagatatag
    27841 ccattgattc tgggtacata agtttaccat gttcttcaac tattagatag ttgtgaacta
    27901 tagcagcaat tcctatacca aataaaactc caccaactcc aacagctaag ccttcaaaga
    27961 atgtaatttg gcttccaatt aatataactg ctggtaatac gaaaataaat ccacttgcaa
    28021 cagattctcc accacttgac ataccttgga ttaagttttt accaagaata ccctttgaac
    28081 gagcaaatgc tccaacaaaa gcagaaccta ttatagctcc tggaatacct gcggcaactg
    28141 taagaccggc tttcattcca gagtaagttg tagatgcggc aaaaatggca gctaaaataa
    28201 taccgataat cagtacagct acatttccac cggttctgga cttatctgtg atatatggaa
    28261 cgtaatcttt tccactaacc tcaccataag cacccttagg taattttttg ttcataaaat
    28321 tttcacccct tttcaatttg tatatattat aacatatata catataaaac ttaacttttt
    28381 attattttac aaatagcgag tcaaaactac taaaatttaa ccctatatta ttaatttcta
    28441 cttttagata tttttatgct aattttcttt ttatttttca attctactaa aaactattaa
    28501 gatataaaat aaaaaaattt tttaatgtaa aattaatttt gtaaaaaaaa tgctttaaaa
    28561 cacggaaaag tataaaaaat acaataatca ttcttataaa taaattttca ccatagtaaa
    28621 tatatacaga aaattcttgt ttttatgcaa attgattctt ttataaaaat aagtgtcata
    28681 aagaaatgtc tgaaatcact ttatgacacc tatcataata aaaaattatt ctggtaatgc
    28741 tcttttattt acttttccga ttggtgtaac tggtatctta tctatcctca ttattattgt
    28801 tggaatcata tatggaggta gatgctcatt taaatattct ctaatagttc tttcatttat
    28861 cttaccacta tcttcctcat caattacata atatgcacat atatacatat ctccatcatc
    28921 atttgttctc gatatacaaa caacactttt aatttctgta atattattta ttatattttc
    28981 aatctcatat agctctatcc taaactcatc aatttttacc tgattgtcca tccttccaat
    29041 ataatctaaa tttccatcat ctaaccatct tactaaatca ccagttctat acattaactt
    29101 attatgttca caagttttgt aaggattctc tacaaatact tgctctgtta gccctttttt
    29161 atttaaatat cctcttgata tttgaatgcc tgatagacat agctctcctg gtgctccaat
    29221 tggacatagt ttgttctttt tatctaatac atatgcataa gtattaaata taggttttcc
    29281 tattggtatt tttccatatt ctctgtccac atgaaaagaa gtcgatacaa cagtaaattc
    29341 ggttggtcca tactcattat atagttcata attacgcttt ctatatcctt ttaatctttc
    29401 tcctccggtc tgtacaactc taagtgaatt attattgcaa ttttgtataa ataaccttgc
    29461 aaatgctgtt ggcaatgcta gtatagttat gtcattttcc tcacagtatt catttactgt
    29521 atattcatca aatttagctt cttcaggcat aatatctatt gatgcaccta caagcaaata
    29581 agggaatagt tgtttcatta cagcatcaaa actgaaactt aagtatatac cacatatatc
    29641 attttctgta gcgcttatag attttacgct ataataacac atatttacta aactcttatg
    29701 ttctatagct atggtctttg gcctaccagt tgaccctgaa gtagatatcg tatatgcaca
    29761 atttgatgat gttatttttt cagttaattc atatccaagc ttaaacattt cttcatcatt
    29821 tatatcgagt actatggtat tctcatttac aaccttactt ttgagttcat ctgtagttat
    29881 cactactttt gcattactat cttcaagaag gtaattcaat ctttcttctg gattacttgt
    29941 gtctattgga aaaaatgctc cccctgattt cataatccct attgctgtaa taattatgtt
    30001 aatatctctt ggtaacatta cagctactat gtcttcactt tccactccta tatgatttaa
    30061 ataacctgca agtttatcag ttacttcatc caattcttta tatgttaact ttctattttc
    30121 atccctagca gctatattac taggtgttac cttaacttgc tccctaaata aatcaacata
    30181 acttttatca tatggtattt catattgatt attattaaag tcttccaata atactttttc
    30241 ttcttcctta catacaaatt ctaaatcttc tattaaaata tcattatctt ctattatttg
    30301 ctctattata taatcaaatt gatttaaaat tcttctaata gtttctcttt tatatataga
    30361 tggtttatac tttattttta ttttaagtgc cctcttagta tatactatta cagataaatc
    30421 atatcctttt ttttctatgt cataatcata tgataaatca aaacttttag ctataaatat
    30481 tgtgtttata agcttagtat ttagatgatt tttattctct atatctgata acaaatagta
    30541 tccatactct aaagacctgc tccactgcct ttttagcttc cttacaacat ctataaattt
    30601 ttcactttgt ctaactctta ctcttattgg aattatattc attgttttac caaaaactac
    30661 atcatcttca tatatatact tttgaagtat aaggccatat atagtttcta tgatagagtc
    30721 tatgctaata ttgttcactt tagaatattg aattaacctt tcatgtttct caatattcat
    30781 cttaaaaacc tcatctacta cttcatcaga acaatcttta taaaaaaaat catttggaat
    30841 ttttgctata ttcgtataat catctaacaa atccatccaa taattcatag ctcgttctaa
    30901 attaacttca tacatatcaa aattccccct tactactaag tctatagaaa aaattctttt
    30961 ggaattttat tttcacaata tataaatata ttaataatac atatgcaagc tctaaaaaca
    31021 tgagcttgca taaaattaca cttattatga ctactaaatt cgtaatattt acaatgttta
    31081 aaatatatta taacacatat atgattttat ttttctacat ttatataata tataaattac
    31141 aaatagttat ttaactaata tataacaata ccttttggaa tatgatatat tatattaatt
    31201 aaatttttat aagataagtt atacttatag gagggaacca tataaatgaa tatttatgct
    31261 catagggggt ttagtggaaa gtatccagaa aatactattc tagcttttaa aaaatgtttg
    31321 gatatggata tatatggaat agaacttgat gtccacagaa ctaaggatgg aaaaattgta
    31381 gttattcatg atgaaaaggt tgatagaact tttaatggac atggttttgt aaaagacttt
    31441 actttaagaa aattaaaaac tctaaactca tcttttgaag gatatcaaag caataaagaa
    31501 tgtaaaattc caactttaga agaagtatta attctaattt cacctacaga ccttatttta
    31561 aatatagaac taaaaactga caaaataaac tatcccaata tagaaaaaga cgttttagag
    31621 cttattttaa agtataatat gaaaaataga gttcttatat ctagtttcaa cagcaatagt
    31681 ttaaaaaact ttcataaatt agaccctagt ataaaaacag gattattatg ttatttacct
    31741 ataaacaatg tagtcaattt tgctaaattt ctgggtaata gttatcttca tccacctcta
    31801 gttttagtaa atgaatctct aattgaactt tgtcacaaaa atctacttgg tgtcaatgta
    31861 tatactgtaa atgaagaggg tgatatacta cactgtctaa aactcaatgt agatggtatt
    31921 tttactaact atccagatat tgcttcaaat cttttacact caaaacaata cagctaatta
    31981 tacaatagtt tgtactcctt gacttttata taattaatat gtacaatttt attaaattta
    32041 tcttggaggt ataactatgg ataacttatt aaatgaactt gaagctttaa agagtaattt
    32101 agataatcta ccaatagaaa aaatagaaaa ttatgatttg agtaaaactg ctttatttat
    32161 aattgatgtt aataatggct ttgcaagaca aggtgcatta tactctccta gagtagagtc
    32221 tttaataaag ccaattgaaa tgtttacaaa aaaaatatca aacaaattaa atagagtaat
    32281 agcatttact gattctcata ctcctaaatc aatagaactt ttgagttatc ctgttcactg
    32341 tttggaaaat gatgtagaaa gtgaattggt tgatgaactt aagtcgatag aaaacctaca
    32401 aattcttcct aaaaattcta ctaatggatt ttttgcatta gaaaatttgg attttgataa
    32461 tatagataat ataattatag ttggcgattg cacagatata tgtatatatc aatttgcaat
    32521 aactctaaaa tcatatttta atcaacataa tattgaaaaa aatatagttg tacctatgaa
    32581 tttagtagat acttatgata ttcctaacgt tcaccctgct gagattttaa acttagtatt
    32641 tttcaacagt atgattcaaa atggagttaa tgtattaaaa gagattcgtt aagcttctaa
    32701 tactttacat cccatataat acaattatca gatatttatt tttaataaaa tttctaatag
    32761 aaaaacacag ttttagtgag aagattacaa tttaaaacac tatctattat tactaatctt
    32821 cacaatataa aaaatcgcag tttagatatt ccttatacaa atttataagg aataataaat
    32881 tgcgattttt atttttaagt agtacaatta atttatacta cttcagtatt taaattcatt
    32941 ttttaataat catacttatt cgtagtttcg cacattacta cctttatttt tttattaaac
    33001 atattcttat attctaataa aaactcattt agttctgaat ccataagatt tatttttcta
    33061 tcactacaaa gcattatctt ttcttggtcg attattacat taatatcatt taagtcattt
    33121 tttataaatg ttgttataca actctttggt tggatttttt ctccatattt ctcataaaat
    33181 cctgcactct tcttaaatga ctctaaatct tctgtagata ctttgctgga agtattatta
    33241 ctcataaaac ctgctgtaga agggtcgatt ccaaagtttc taagttgttt ttctacttct
    33301 tcagtgtcca taccatatct ctccataaat tgcttttgta ttttcataaa tttttcttga
    33361 gatatgttgt tatctctcat ataatcgaat atttttcttg aaaactcagc catagacatc
    33421 tttccatcca taacagaaaa atataagtta tatatgtatt gctcaaattt atctacatca
    33481 gaatctttaa ctttgttctt tagttcgtta aagtctatta ttctatcatc agacattttt
    33541 catcctccta gctaaatttt tacttgtata agattatacc atatttctat tatatttata
    33601 tacacactat ttttttaatt taacgatttt ttctgtagca tctaattcgc acattctgcc
    33661 caaaggaata gttatcattg ggatacaatg tcctgactga agattatata tagaaggttt
    33721 attgacctta tttgcaaatt ctattagtaa ttccatcaaa tcattttctt caatacaatc
    33781 cttaaaatct ccaaaaatta taccattaca ttcctcaagt tttcctgaag aatatagttg
    33841 tgttaacatt ctatccagtt tatattgagg ttctccaatt tcctcaataa acaaaatttt
    33901 atcttttgca tctatttcat attttgttcc cattgtggca attattaagg ataaatttcc
    33961 tcccattatt attccctctg ctttaccttc acatacagta taaatctttt cttcttttgg
    34021 attttctaaa tataattcat ctttaaattc taaagcattt accaatgatt tataggtaaa
    34081 tttaccccat ttacctacat ttgctgccat tattccatga tatggtaaaa gaccacaatt
    34141 tttatagaaa gctaaattta aacctgttat atcagaaaaa cctataaatg gttttggatt
    34201 ttgctttatt atatcataat ttattttatc caatattctt gttgttccat aacctcctct
    34261 taagcacata attatatcta catctttatc taaaaacata tcttctatat cccttgctct
    34321 aatatcatca ttaccagcta aataaccttt ataatttaaa ctacaacttt cagaaaactt
    34381 aacttcgtaa ccataacttt ctaaattaaa ctttatttcc tccaaacttg tatttcttaa
    34441 tggcccagat ggcgcaatta ttcctaacat ttaaacacct cttcttataa aatgatatat
    34501 tcttctccat actaaatatc tctaatataa ttataagata ttttatttgt acttgataca
    34561 taataatgat ttacataaaa taatataatt aatgaagaaa aataaatttt taatatagtt
    34621 aggatgtgtt ttatgcttaa tataaaaact ctttgtcatg agataaatga ttgggttata
    34681 aatataagaa gagatttaca taagactcca gaacttggat tagaagaatt tcaaactaag
    34741 aaaaaaatta ttaaatactt aaatgaaata ggtataaatt atattgaata taagaatcat
    34801 acagggataa ctgcatacat taacgtgagt ccaaactttg aaacagttgc tataagagct
    34861 gatatagatg ccttacctat aacagaagaa cttaattatt cttataaatc tattaatatt
    34921 ggcaaaatgc atgcatgtgg tcatgatgca cacactgcta ttttactggg tacttgtaat
    34981 atactattta aattaaagga ttacttaaat gtaaatgtaa aattcttttt tcaacctgct
    35041 gaggaaacta ttggtggggc tcaacttatg atagaagatg gatgtcttga aaaaccaaat
    35101 gtaaaatata tatttggact ccatgtaaat cccaatatca acaaaaatct aattgaatta
    35161 aaatacaaca ctctaaatgc ttccaccaat acacttcaat taactgtaca tggttcaaaa
    35221 tgtcatggag cttatccaca tcaaggtatt gatgctattg ttatatctgc tcatattatt
    35281 acagctctac agacaatcgt tagtcgaaac acaaatccaa ctgattctgt ggttatttct
    35341 ctaggaaaga ttgaaggtgg aatcaaagaa aatatagttt gtgacaaagt ggttataagg
    35401 ggtactttga gaaccttgac tcctgaaact agagagtttt caaaaaaacg aattagagaa
    35461 atttgcgatt ttacttgtaa aacatttgga ggaagtattt ctgttgaaat tgaggaaggt
    35521 tatccagctc ttatcaattc aaaccatcta gtagattacg tcaaacaaaa tgctgtagaa
    35581 ttgtttggtg aagaaaatat tatccttaaa gactcgccca ctcttggtgc tgaagacttt
    35641 tcgtattttc taagacactg tgaaggagct ttttaccacc ttggttgtgc aaatcgtgaa
    35701 aaaaacatta cttccccttt gcatacctct acttttgata ttgacgaaga ttgccttatt
    35761 actggtgtca tgctccatgt aaaaaatgta ttatcattct aactattaat atttttattt
    35821 attattttat attgaccctc acatgatgtc atgttatacc ctaaaattac aaatgataaa
    35881 ttaaatagtt ggaggtaata ttatgaaaga aatattaata aatcctatag gtaaaatatg
    35941 tattcaaggc gaagaggtct ttattaaatt agacaaaaag tatgtacctg cattaaatga
    36001 actcgacgat ttcagtcatt taaatgtatt ttggtgggct gatgggtttg ataatcctga
    36061 actaagaagt attttagaaa ccccaaaacc ttataaaagt ggaccagacg ttataggaat
    36121 atttgcaaca aggtcaccta taagacccaa tccaatagca ttgactgctg tacaaattat
    36181 caatatcgac catgaaaatg gcataataaa aataccatat attgatgcta atgataatag
    36241 tcctgttatt gacttaaagc catatactcc aagcgttgat agggttgaaa atccgtccgt
    36301 tcctaaatgg tgtagtcact ggcctaagag tgtagaaaaa tcaggagaat ttaattggga
    36361 agatgaaatt gaattttaaa ttactttttt cttgatttca ttaacttgtt ttccacattt
    36421 attttattta cacttaccaa gattttatgt cacatataca tataatatta ggctgtatta
    36481 tataatacaa cctaatatta tataataaat ttctattttt cccttaaatc attcacattc
    36541 tttctaagct acataagatt tttctattat ttctttttga gtttaattta gcattatgtt
    36601 gaggaaaaaa catttcatgc attttaataa attaaatttt tcttttattc tgcgatttta
    36661 taataatcaa tttaacttaa aaataatacc taactatttt aactatgtta aactagtaaa
    36721 tgtaaccact tatatgttac aattttatat aaacatcaaa tataaaaaac tatataaata
    36781 aatgacacat agtgttatat ttttttatac caatttagtt atttttcagt tacagattta
    36841 ataatagtta atagtaagac ataatatttt tatgcccttt attgattatg tattttctgt
    36901 tatctgttat tatcatcaat aataaacata agggggtata tattatgaaa ttgtatttaa
    36961 gtgctgatat agaaggtact tgtggtattg taaattggga tgaaacaaaa cttgaatgtg
    37021 aattttcgca acactataga acgcaaatga gtaaagagat aacagcagca tgtgatggag
    37081 ctactcaatc tggtgtacat gaaatacttg ttaaagatgc acatgacagt ggtagaaata
    37141 ttaatccaaa tattttacct gaaaacgtca aaatacttag aggatggacc aaagacccac
    37201 tagttatgat ggcaggaatt gatgaaagct ttgatgcatg tatattcacc ggctatcact
    37261 ctggagctac ttgtaatggt aatccacttt ctcatactat ggatattgat tatgactatt
    37321 ttaaaataaa tgatgaaata gcatctgaat ttactataaa tgcttatatt gctgcatatt
    37381 ataatgttcc tgtagcattt ttaagtggag atgaaatgct ttgtgagagt gcaaaaaaac
    37441 taaatccaaa tatagtcact gttcctgtgt caaaaggaat tggaaatggt tcaatttcaa
    37501 tccatccaaa ccttgcacta aaaaaaattg aaaaaggtgt caaaaaagct ttatctggag
    37561 atttatctag gcacttaata aaattgccag ataaatttaa gatagaaata aaatttagag
    37621 aacattataa agcttttaag gcctcttttt atccaaatat gaaaaaaata gattctcaaa
    37681 cagtactatt tgaaacagag gactattatg aatttttaag aatgttatta tttatataaa
    37741 tttcatatca taaattaagg agttgattaa gataagtaat aaaagaagga ttcctttagg
    37801 cgaatttatt tctgtatttt tattttttgc tgtttttttt ggtataactt tttgtatttt
    37861 cacggttata ggaattggct tcttatcatt cttaggattt gaatataaat ctcttggagc
    37921 tgtattaatt ttctttttaa tatatttttg tataaccact ccaattgatt ttctatgcac
    37981 tactatttta gatatattca gatatgtaaa caaattacca tactcaatat ataaactttg
    38041 cgaatttata attgatttta tactaacttt tctagctatg aacataattg atacctttat
    38101 ggattcagta acgattcctt taagtacaga aatcttattc gctttacttt cttatctttt
    38161 atcagaatgt atggattttt ttgataggga taagaaaaaa ccttaataaa aaagatagat
    38221 tccttaatag aacacatatt ttcgtatttc tattatttaa tctatcttta ttttatttaa
    38281 taaattttta ttaattaaaa tatagtttct tctatctcct tagtcatttg cttagcacta
    38341 tcaaaaaatt cttctatata cactacttca tcactgaccc tagaatattc tgctgcaaat
    38401 gccattacat gactttgtat agcttctttt gctgctactc tacccttttc tagttcttta
    38461 tttgaaacta atttaataaa ttcagctata agatttctat catattcctc taccatattc
    38521 tgttgatttg atggattcat aactatttct tcgttttttc caaattcttt aattcttatc
    38581 tcatttttat ttaaatcccc accaacttca ccatggctaa acattaactt tatagttctt
    38641 ccattttctt tagtaaatgc acataaatta aaagtagccg tcactccatt ttcaaactgt
    38701 agtatattta ccatattgtc tacaacatta ttatcacatc tgtaaacaca tcttccatat
    38761 ggacttgtct taagtatatt caatagattc tcttcagttg gatttatgtg aacagctcta
    38821 tttatacttc tatctttttc taaataaatt ctctttgctg aatatggaca ttttttttca
    38881 actaaacact gaagacaatt ttgtgccatc tctccactag cattttggtt attaagatgc
    38941 tttaagcttc caaatgcaga tatttttttg catctacttc ctactaaata taacaaaata
    39001 tccatgtcat gacaactctt tgctaaaatt aaagggctgg tttcattgct atttctccaa
    39061 ttccctctag taaagctatg tgcaaaatgc caatatccta tattttcatt atattgtata
    39121 ctaactagct ctcctaattt tttgctttct acaatttctt ttaatttatt ataaaatgga
    39181 gaatatctca gtacatggca aatcataaaa atcttatctt tatacttatc acacaagtca
    39241 tctatatgta ccagaccatc taaactatta gacattggct tttctaatag tacatgataa
    39301 cctttttcaa gagctagttt tgctacatca taatgtctat catcatttgt agctattatt
    39361 actgcatctg ccatcttgtc atgtttaaaa aagtcttcta aagtttcaaa tacattttta
    39421 ctatctaggt tatatttttg tgtaaatagt tctcgtcttc cttttttagg ttctgctaca
    39481 gcaactattt cacatacatc agaattttca taaatatatg gagcatatgc atacgttcct
    39541 ctattacctg ctccaatcac agcaactttc actttttcca tactttaatt tatccccttt
    39601 atattattta tactactcta ttatagtcta tttttacaga catttggata tttttaataa
    39661 aagaaacagc agttataatt tttcaatttt aactgctgtt ataagttaat ataccaaaca
    39721 tttttaatta aattttcttt aattttaata caaagactta aatattaaat acaaattata
    39781 aaaacacttt tatctagttt acttcttctt ttctttgtct aatttatcta acttcttatt
    39841 gtataaaaac atataaaatg ctgccgaaaa tactattaaa tatccaacaa cacttaacat
    39901 atctggatag acattgaata agaatatact taaaattgct gaaaagatta tattactata
    39961 gtcaaatata gagatttcct tagcaggtgc atatttatat gctagagtta ttccaaattg
    40021 acctagactt gcaaatactc cacctagtaa tagatatata agttgcataa gggtcattgg
    40081 ttcatatact gcaaaagcaa ttggccctac agtaactaat gaaaaaactg aaaagaaaaa
    40141 tacaattgta tagtaatctt ctctatctcc aagaactctt aaacaagtat atgccattgc
    40201 tgcaaataca gctcctgcaa ttcctccaag atatggaatt acttctacac taaatgttgg
    40261 ttttattatg aataaagctc ctataaatgc aattataata gctcctatct gctttacatt
    40321 tattttttct tttagagcaa ttgcacaaaa tattactaca aggaagggac tcattttatt
    40381 tagcatatta gcatctgaaa gcatcaactt gtcaattgca tagtagttaa atataactcc
    40441 tagagttcca aatattgacc tatataaaag tacttttaaa ttttcttttt taccagttag
    40501 acttccttta tgttttatga taagccaaaa agctattata gctgatacca gatttctaaa
    40561 gaacactttt tgaaaacttg gtaaatctcc agctagcttt acgaatgcag acatcattgc
    40621 aaagccaagc gctgatgcta ttataaacat tatacctttt attcgatttg atttgattgt
    40681 gctactcata cgtacctctt ttctatataa atgtataaag ccatattaat taaaaactta
    40741 gcttatacat taaataataa ttatctaaat attccaattt atactataat taaaagtgta
    40801 aactatgtca agtaactaat aaatcttttc aattaataac aataataatt atagctaaaa
    40861 agttatatgt ttttatggat tattatcaga atatataata taattttata tagtatagac
    40921 tttttttaat ttattattta gatttttaat agatttttta actatggagg tatttaaaat
    40981 atggaaataa agagatatga aggaactgga agaatgagta gagctgtagt tcataacaat
    41041 acagtttatt tatgtggaca aactcatgct gaaggaggag taattgaaca aacaactgaa
    41101 gttttagcta aaatagaaga tttattaaac aaatatggtt cagataagca acatttgcta
    41161 tctgttacta tttatttaag agatatgaaa gactttgaag ctatgaactc tgtttgggat
    41221 gcttgggttg aaaaaggatt tgaacctgct agagcttgtg ttgaagctag acttgctaga
    41281 gagcatttgt tagttgaaat gagcgttgtt gcagctacaa aataatcaat ttagcttata
    41341 ggaggctatc tcaaaacaga gagataagcc tctttttcat tgttgaaatg atgaaaatat
    41401 atttgatttc gtataaaaga aagtgtctca cgaactaaaa aattcatttt gagacacctc
    41461 ctcactattt aatctatata aatttactta ttctctattg gcaatatcaa tttagatgga
    41521 tatttacctc catggtaaat cgtattcttt gcttctatat attcaatact attatatccc
    41581 tctgtcgtgt tgctattttg gaaaataaag tttttagcac tagacgttat atctagtcta
    41641 attttatgac caactttaaa cgtgtttgat atttttgaag ttataatcgt aaacttataa
    41701 atctctcctt cttccataaa ctctgactta taaaaactat ttctaaatct agcactaagt
    41761 ataccatcag ctaatttaat tgaatttcca tctaaatcca cgtccataat ttttataacc
    41821 cagtcagtat ctttggcaga acttgatgca aaaaactcta actttatatc acctgttaca
    41881 gtaaattctt cactaaaagc atctgatgta taacacaaca tatcacttct tttttctaaa
    41941 tctttgtagt tatttggaac tccaacttca ttctctgaca tatcaattag ttgtactgat
    42001 gggtcttttg gattatatat ataactatca tacttttctt ctaaatcatt atcaaatact
    42061 aatctaccat ttcctaaaga tgtgttagca tttccatcac ttattagata catactttta
    42121 tctatcttat ttattattgg ccaattttct tctgtcttcc acttattaga acctacagag
    42181 taatactcta ctggagcagt cgtatctatg ttattttcta taccttttaa gtatttatca
    42241 aaccataata gataattata atccaaatca tatcttaatg aactatttcc taaagaaata
    42301 ccatttatat ctcttaaagt atttgaatta tgcatccatg gacctagaat agcctttctc
    42361 tttccttttt catagtcctt tataacatct aaggcctctg tagttcccat actattatca
    42421 tcataccacc cagatacaac caatgcaggt acatttattt tatctttttg taaatgccag
    42481 tccatcaaat cccaatactc atctttttct actctcttta accattcttc cagaaattca
    42541 attctatatc caagagcttc aacaggtata ttttctattg gtcgtatgtt taaaacatca
    42601 tcccaatcat ctcttaccat tttgcttcta tcaactttat ttcttgatac cataaatgcc
    42661 catgcaagca taccagatac aaaagctcct ccttttcttg gtatatcaat aaaagggctt
    42721 cctgatgtta ctatactaac taaagcttta agatgcttat tgcctgaaga tgcagctgcc
    42781 cattggacat aaccaagata tgatgctcca agcattccaa ctattccact tgaccattcc
    42841 tgtgatacta tccaatttat ggtactatct ccatcttccc tctcatgaat cataggttcc
    42901 cacttacctt ctgattcatt tcttccccta acatcttgta ttacaacagc atatcctctt
    42961 tgtacatatt tataatatat ttctttatca ttttcttttc catatggagt tctcataaga
    43021 attgtcggtg cttttttggc agaatctaca aaatcaggga gatatacatc tgtagaaagt
    43081 tttataccat ctttcatatc aatcatataa tttccaccaa atcttataga atatatctct
    43141 tcatttttat gagttttttc ccattctttt aatacagtat actcttcata tccatccata
    43201 attaatatag tacaagtttc tctagataaa cagataaaac ctactatttt attatctaca
    43261 ataattatat ctagtgggaa ctttttgttt ctttgaaccc atatatattc attaagttta
    43321 gaatgcgttt ctcctaagtt atttataact gtgtctttat ctgtgtttac taagctttta
    43381 gtatatacat caaaattatt aaataaagac tttaaatcta tttttgtaag atttaaataa
    43441 tatgtatttt cttctttgct tattggtttt aaactttcta ttttatttgt aaaaggattc
    43501 aacttttcaa ttaataaatc tccatcaaag attacatttc cactgtatag tccagatatg
    43561 tataatttaa attttatcaa ttaaactccc ccctataatc ctagagacat ctttattgca
    43621 tatgccactg atatacctgc ataatttcct aatactccac caattactcc cattacgact
    43681 gctacactta caagcgaatt ccactttgca gaagcagcta ctaaagctga agtaggacca
    43741 tcagcaattg ctcctactat agaaagtatt acatattgat actttatctt taaaagtcta
    43801 gttatgccta aatggaaaac aaatgaagca aaaattatac taaaacagta aagtgttata
    43861 gtaaaagttg aacctaaaaa ctcttttata tctactgaaa aacctattgt acataaaaag
    43921 aataatgcaa caaataatcc taaatctaaa tttcccttta gtttctttac tggctttaat
    43981 tgagcaatta taattgaaat tgttgtaact aaaagtattc ttgctgcact actaaaactt
    44041 gaattgaaat atccagcaag tattgttgaa acttctgtaa caacaaaacc tatagccaac
    44101 atccaagcta tatctttaat actccattct tttgactcca taaattcttc attttgacaa
    44161 tcttctaatt ctgattcttc tacatgatat ggccaaagtt ttttaaactt ctttgaattt
    44221 tttaatattg ctggtagagc aaacataaat atcaatgttg gtactccaat tacatagtct
    44281 gctgcatttg ctgctgcaag agtttgtcta cttacattaa gacctgttcc aatagctgta
    44341 agattagcac ttccaccagt atatgtacct acaaacattc cagcaacctt ccaaccttct
    44401 gatatttttt ctgcaaatac taatccaaat aaaaatgcca tgatacttac agttaataca
    44461 gctacaaaaa ttgcaattaa aggttctctt gataattttg tcatttcttt taagtcaaca
    44521 ctcatcaaca ttatagtcat agaaattgga atagcatatg ttgaaatagt tccatataac
    44581 tcagtactta ctggaactac ttttaagttt gaaagtatta ttcccattat gattattgta
    44641 agtgctggtc ctaagctttt aatggctttg tatctttgta agtaaaaacc caatgcaatc
    44701 attgatgtca ttacagcaag tatactacct ggatttgaaa aaagtgtatt catatttaac
    44761 ctccaaattt atcataagtt tacttctatt cctaaacctg gtttattgac aagattcata
    44821 atatctccat ccattacaaa tcctccagat ataccttcta attcttcaca aaacataaaa
    44881 ctatcaaggt cagcttctgt tatgttcttt ttagatgcta ttaaatttgc tgctgctgtt
    44941 ataccaattc tcgtttccag catacatcct aacatacaat ttactccact tgcctcagct
    45001 atgttgttga tttttatagc aggatatatt ccacttgatt tcatcagctt aatattaaat
    45061 gtatcaactg cattcttttt tatagcctta attgcatcta ttggtgaatg taatgattca
    45121 tctatcataa tttttgtgtt acttttattt cttatgtaag cagtaccatc taaatcccaa
    45181 tgtggaagtg cttgttcaat tgcatcaaca ttaaattctt ctaacttttt gatagtattt
    45241 atactgctat ttacgttcca tccttgattt gcatcaattc taattcttat actctctcca
    45301 acggcttctc taattaactt tacagcttca atatcgtctt caggatttat acctgcttta
    45361 agcttcaata tcctaaaacc ttcttttact ctttctaaag cttctactgc cattttttct
    45421 ggcttatcta tacttatagt aatatccgtt tggactttac tatcaaatcc tccaagaact
    45481 ttataaagag gtgcattcat aatttttcct ttaatatcat ataaagctat gtcaatagct
    45541 gcttttgcag aagtattacc tataataatt ccatccatga tggtatgtat ggtttcaata
    45601 tctagtgggt ctttgcctat taactctttt ttaaacataa gcaatactga aagaacatta
    45661 tccaaaactt cacctgtaac tgctgcaaga ggtgctgctt cgccaaatcc acaataccct
    45721 tcatcagttt caatttttaa aattatactt tctccatact caattactcc aaatgaaact
    45781 actacaggtt tttttaattt aattctaagc ttttcaaatt tgatatctgt tatcttcata
    45841 ttaccctcct tattttttga agttataatt caatttttaa aattttatac tttaaattat
    45901 attattactt caatatatat gtcaagataa tttactgaaa atttactctt ttccaaaata
    45961 taatgtgata atcttaattc aattaatact cttctattaa atttttttat tattattttc
    46021 tttgtattaa tttaacaata attcattttc atagactttt atttggattt aattaaattt
    46081 taattgtcaa aatctactat gtattcttat tgccactact tctatgttgt gctataatta
    46141 ctttgtttta agcgaatatt ttatattaat tttattattt ggaggttctt atgctaggtg
    46201 aaaagatgag aaatataaga aaaagcaaga aaaaaacatt aagtgatgtt tccaaattga
    46261 cagatttatc tataagttat atctctcaaa tagaaagaga tgctattgaa ccatccttgt
    46321 cttcattgag aaaaatcgct gaagttttag acactccttt atatatgttt atggatgaca
    46381 ataatactga tgatttagtc attagaaaag aagatagagt tatgatgaaa tttccaaaga
    46441 gtgaaatgtt ttacgagata gtatctccaa tgcctacaac tgagtttaca ccttcaattt
    46501 tatttgtaga gtttgaactt aaaccagaaa gtgaggatgc caaagattat atatatcatg
    46561 catcagaaga aattatggtg gtaacgagtg gtgtggttga tatttgtatg ggtgaaaagg
    46621 tagttagatt aaatccaggt gactcaacat ttataaaagc taatgtacca cataaaatca
    46681 tcaatccaag taaagataca atagctcgtg gatatggagt tatatcacct ccaatttggc
    46741 caataaaatc taaataaact caatatgaca cacctacttt ttgtagataa tataatagat
    46801 ttaaatattg cttttatttt aaatttaaaa tatttactca aactaaaaaa atagctattt
    46861 gactaatcta aaccatctta attatggtaa atagtaaata gctatttgta agttatatta
    46921 taaattataa taaaaattgt atttgttaaa attaaactgc tatatcattt aatacttttg
    46981 cctttacaac tcttatattt ttcatactct tgtagatatc ttgaatctta tcttcttgct
    47041 ctctcaatag tctatgtgct tcatatatat ctacaagttc aaattttact acatcatttg
    47101 gctttgcttg tgctaactta tacaaatcta cacttataac gttccctatc ttagtatagc
    47161 caccactagt ctgtctatct gataacatta taatcggttt tccatgacca ggtacttgta
    47221 tagctccata agaaatacca tcagatataa tatctgctcc attaaggtgc tttatactct
    47281 ctccatctag tctacaaccc attctatcta tttcattcgt aactttgtat ttatttgata
    47341 aaaatttctc tacttcaccg ttagaaaaag catcaaattg aggacctttt acaattctta
    47401 gtacgatttc tgaactataa gttggaatat attttgaact taatttttta tttattgtaa
    47461 aatctttttc ctgaataaaa gtatttatat aatctccttt tttaagtgct ctaccttcaa
    47521 atccaccaat tttagctctt gtataagttg acttacttcc cattgcaagt ggaacatcta
    47581 ttccaccagc tattgacaca taggctctag cacctgattt cacacctcta aatgataata
    47641 catcacctgg atttatttga tatgacctcc agttttgaat ttcttttcca tttattttag
    47701 cccctaaatc tcccccagtt actgctattt gcgtatattc atcaaaggta attgtaggac
    47761 ctaacattaa aacttctaaa gaagcctcat cttcagaatt tcctactaat atatttccta
    47821 ctctatgtgc atactcatcc attgctccac atccagatac accatattga ccatagccaa
    47881 ctctacctct gtcttgtatt aatgtttgga aacctcctaa ttcaactttg aatcccatat
    47941 ataccactcc ctgtactata acgatattta aatacttaag ttattttttt cgatttctat
    48001 aaattcatct aatgttattg gaacaaattt tataaaatct ccagctctaa gcaaaatctg
    48061 ttcttctcta ttttcatcat ataattttaa aggtgtccta ccaattattt gccatccacc
    48121 aggactatca ataggatata caccagtctg ggaacctgct attccaacac tcccaccata
    48181 aattttagtc cttggcactt ctaatctagg tgtagctatt ctttcatcca taccacctag
    48241 atatggaaat ccaggagtaa aacctagcat atatatcaag tactctctgc ttgtgtgtat
    48301 ttttataact tcatctacag tgagattatt gtagcttgca acgtttttaa tatctatacc
    48361 aaaaacctca tcataacata ctggaatctc atgtatttta ggttttggta atactatact
    48421 ttccaaatta tcttctattt tttttaaatt ctcaatcaaa ctatcaaaat ctatttctaa
    48481 tggattataa cttatcataa gtgacctata tgttggtacc atttctgtta caataccttt
    48541 aagattcgat atttctattg ctctcataaa tgaaattact tttttgttta tatcttctga
    48601 tatctcattt ccaaattcag caaccactgc cttatcccct gatatcaagt atctagtttc
    48661 cataaatgtc acctcttcta aattaagcaa ataaagcacc taggctgcta agagaagtta
    48721 ttcctgtata tgcagatatt aaaactacta ttaatccaaa tattaaaagc catgtaggat
    48781 gcttgtaatc tccaacaata tcttttcttt tagatgctat aagcataatt cctaatgtga
    48841 ttggaagtat caatccattt aaagctcctg ctaaaattaa taaagtagct ggttttccta
    48901 taaatgccat tataagtgtt gatattgcta taaatgctat tataaaatat ttttcatatt
    48961 tatctataaa aggatttagt gtctttaaga aagaaactga tgtataagca gctcctataa
    49021 ctgatgtaat tgcagcagac cataatacaa gtccaaagaa tttatatcct attgctcctg
    49081 cactaaattt aaaagctgaa gcagctggat tttctgggtc aagttgaaga ccttttgaaa
    49141 ccactgccag tattgctaaa aataaaagaa ctctcatcat tgtggcaact aatataccca
    49201 aaagagaaga tttagttatt tcttttatat tttcttctcc tgttattcca ccgtctatta
    49261 atctatgtcc tccagcaaaa gttatataac ctccaacact tcctccaagt agagttatta
    49321 ttgggaatat cagtcctttt ggattttctg gttttaccat tcttgatact gcctctccaa
    49381 caggaggatg atttgatatt gctacatatc caactataat tatcattcct aatgctaaaa
    49441 atttagttat cttatcgact agagaatttg aattttttga cataaataca aatatggcta
    49501 ttaatccact taatacagtt cctaatgtca tattcatatt aaacattaca ttcataccaa
    49561 gtgcagctcc accaacattt cctatattaa aagctaatcc tccaagagct actaaaaaag
    49621 ctactaaata acctaatcct ggtattattt tatttgctat atcctgacct ctaagtcctg
    49681 atacaccaat tactctccat acatttactt gagcccctat aaaaaggatt gttgtaatta
    49741 aaattacaaa tgaaaaattc ggtccaaaat cttgggtaaa ttgtgcagtc tgagttaaaa
    49801 accctggtcc aattgctgat gttgccatta taaatgcagc ccctataagt gctccgacat
    49861 cttttttatt tttagtttta tctgtaatat tttttgtact catattttat ctccccctca
    49921 tgttatcagt cttatactat gttctctgac gaacaaactt ctatgttttc taatggacaa
    49981 acttctatac tttctaattc aaaacgcttt cttatttctt tgacaaactc tattgctttt
    50041 ggattgtccc catgtacaca tatagaatct gctttgatat gtataacttc acctgttaaa
    50101 gtttctacag ttccttcttt taccatccta acaaccctat ctattgcatg ttttgtatca
    50161 tgtataactg ctccttctac atttcttgga accaaaaaac cattattatc ataagctcta
    50221 tctgcaaata cttcatttgc aaacctaagt ccaatttctt tagcagaatt tatcatttca
    50281 ctattatata gccccaacag tataatatct ttatcgactt catatatagc ttcacaaatc
    50341 cccatagcta actctttatc tttagctgcc atattgtata atgctccatg tggttttaca
    50401 tgttgtatat tacatccatt tgaacttgca aatgccatta aagcacccaa ttgatatttt
    50461 gtataatctt tagcttcctg ctttgttatt ttcatctcac gtcttccaaa tcctattaaa
    50521 tctagaaaac ctggatgtgc gcctattttc actccatttt ttttagctag ctgtactgtt
    50581 ttttccatat gactagggtc acctgcatga aacccacaag ctatattagc tgatgaaata
    50641 tattttaaaa cttcttcatc taaaccaatc ttatacgttc caaaactttc tcctaaatca
    50701 ctatttaaat ctactttata caatttatga cccctccttg ctaacatttt gatagaataa
    50761 gtagttttaa tttttaaatt ttgtttattt tatatattgc atgaaatatt ttatcacttg
    50821 tattcaattt tatttttgca actattctat tgcatttaat taatataatt ttaccattta
    50881 aggaatattt ttgcaattat tttaattgaa cattcatttg tatttctttt tcaaaaaatc
    50941 tttttaccat tttttagtta ataattaatt attttcactt aaaataaggg ctttaacctt
    51001 tatttttaaa gtatttttca tttttaatca ctttatatgc aagacagaat catttttctt
    51061 cattcatttg tttctttcat ctatataatt atcattcttt gtaagtaaaa tatctttata
    51121 tttttataat taattcttta tatagtttta agtataataa aatcatgtat actaaatgca
    51181 tgttaaaata ttattttaaa acatgcacaa tatttgtaca tattgtagac atataaacct
    51241 ataaattata caatagtatt gttaagatta tatatttttt taacataaat tttgtattgg
    51301 gggaaaaaca atggataaaa actatacgat gccaatatac caaaaaatag cgttggatat
    51361 tgcaaataaa atatacacgg gtgaaataca agaggattca gtactatttg gacgctctgt
    51421 tttggctggt aaatacaatg tatctccaga aacgataaga agatctgtca aaattttaga
    51481 ggacattggc gttgttaaaa gcataaaggg taaaggtgtt attgtactat ctccagacaa
    51541 agcttcttct tttataaaaa aatatagaga tattacaaat atatcatcat ataaatcaac
    51601 actttataat ttaatagata ctaaatctaa tttagaaaat gaaatattag atactataaa
    51661 taaaatttta gactattcta acagacttga aataatcaat cctttagtgc ccgttcagtt
    51721 caccataaat tctaattgca aatatattgg tcaaacagct gcacaaacta aattttggca
    51781 aaatacaggt gctacaatag ttgcaattaa aagaggtgaa gaacttataa tatctcctgg
    51841 accatatatt gaatttttag aaggagacat tctcttagta gttggtgacc aacacattta
    51901 caattctata ccaatgtttt tatatgaaaa cgaaaaataa attatataat ttagtatgat
    51961 tatataaaaa tgcaaaatta acaacaaaaa aagagcttca tattttacat aaagctcttt
    52021 ttaaattcaa ataaatttaa ctttgattaa tttttaaata tccaattcat cttctttttc
    52081 ccagcttaaa gctctcgata cagctttttt ccatccttta tataatttac atctcttctc
    52141 ttcactcata tttggattaa actctttttc tactgaccac tttgaagtaa tttcatttct
    52201 tccaatatag aatccaacag acaaccctgc taaataagca gctcctaaag ctgtagtttc
    52261 tactattttg ggtctatcta tatctacatt taatatatct gattgaaatt gcattaaaaa
    52321 attattatta cttgctccac catcaacttt taatgatttt agttttaatt ttgaatcgtt
    52381 ttgcattgct tctaaaacat cctttgtctg ataagcaatt gattccaatg tggctcttac
    52441 tatatgttct ttttttgctc ctcttgtaag tcctaaaatt gaacccctag catacatatc
    52501 ccaatatgga gctccaagac ctgtaaatgc tggcactaca tatacaccat tagtatcctc
    52561 taccctattt gcatacattt cactatcttt tgcagattca attatcttta attcatctct
    52621 aagccattga acagatgctc ctccaataaa tatacttcct tctaatgcat atgttacttt
    52681 tccatcaact ccccaagcaa ttgttgtaag taacccatgc ttagatttaa ctatgttact
    52741 gcctgtattc ataagtaaga aacaacccgt tccataagta ttttttgcag taccttcttc
    52801 aacacaattt tgaccaaata atgctgcttg ttggtctcca gcacatcccg caataggtat
    52861 tttagctcct gataacatgc gctcatctgt atgaccataa acatagctag aaggttttac
    52921 taatggcaac atgcttattg gtatgtccaa ttctcgtaat atatcctcat cccattctaa
    52981 atcatttata ttgtacatca ttgttctaga tgcatttgag taatctgtta catgtacttt
    53041 tccacgagtt aaattccata ttaaccatgt atctatagtt ccaaataata actctccttg
    53101 ttctgctttt tccctagctc cttctacatt atctaatatc cattttactt ttgttgctga
    53161 aaagtatgca tctattaaaa gacctgtttt ttcttttatt gattcttcca atcctttatt
    53221 tttaagttca tcacatattt ctgacgttct cctacactgc catactattg cgttataaat
    53281 aggtttacca gtatacctac tccaaacaac cgtagtctct ctttgattag ttatccctat
    53341 tgaagcaatt tcttctggtc ttatacctgc tgtttctata acttctctca ttaccccact
    53401 ttgtgacccc catatttcca tagggtcatg ctctacccat ccagcttttg gataaatttg
    53461 attgaactct ttttgtgcaa ttttcactat ttcgcctttt ttattaaata gtatagctct
    53521 tgaactggtt gttccttggt ctaatgccat tacatacttc ttttccatca ccatattctc
    53581 cttatcatat aatatttaat ttattaaaaa gaaaaattta atatcacata tttcaataaa
    53641 taatcatact taagtacaaa tttaatataa tagatattta gtaagaagac acttattttc
    53701 tatcatatct atataataat attatacatt atagcttaca tattgattaa taaattttaa
    53761 aggagtataa taaacagtgt tcataataat tttaataaaa gaggtggtaa tatgccaaag
    53821 attttagaaa atgtaaaaga agatatatta aaagtgtctc gggatatgat tttagaagaa
    53881 gattactcga atattagcat aaggaaaata gctcaaaggt gtggtataag tgcaggtaca
    53941 gtgtataatt actttaattc aaaacaagaa ataatagaat atatcacaaa atctgaatgg
    54001 gatttattga taaggcgaat tgaatactcc aataaaaaca ctgaagatta cataaaaaaa
    54061 ctaaacatta tttttactga aataagaaat tttataaaca aagttcataa tatccaatac
    54121 aatgactttt taaacacttt tgaaacagag agattttttg aaatgaaaaa acacaaagac
    54181 gactttcatg aacagctttc aaataaagta tatgaagctt tagaaaaaga aagttttttt
    54241 aataatgaca aattagtatg caatatcata ataaaaatgt ttttttcata ttcaacatat
    54301 cttcatatag aatttgaaga tttaaaaccc tatattgaaa aattaataca ttaaggtggt
    54361 gtttaccttg aatcataata ctagtattaa gaacaaggac agtaatccaa aaatattttt
    54421 agtacttaca ctttatctat taggtatatt tatgggagct attgatactg gaatagtatc
    54481 accagcaaga acgattatac aaaatagcct tggagtaaat gaaaaaacgg gaatttggat
    54541 gattaccata tacacattaa catacgcttc tgtaattcct atctctggaa aattagcaga
    54601 taaaatagga agaaaatatg tgtatctagt tagtatattt ctatttggta gtggttcatt
    54661 aatatgtgga ttatcatcac tattttccaa tttctatatt ttattaattg gtcgtgttat
    54721 tcaagcaatt ggtggtggtg gaatcatgcc aattgctaca gcagaatttg gaactacctt
    54781 cccagaaaac aaaagaggca tggctttagg tttagttggg gcaacatatg gtattgccaa
    54841 tattttaggt tcaagcattg gtagcacaat tctaagtata tttggaactc aaaattggaa
    54901 gtggctattt tttgtaaatc ttccaatttg tttaatcata cttattggtg gagttttctg
    54961 tattaaaaac aataagtctg aatctaaaga aaaaatagat aagcttggta cattgatgtt
    55021 gatatgtata atcgtatcac tattatatgg tcttatgaat atagactttt ttaattttaa
    55081 aaattctata caagatatat cagtttatcc atatttattg acatttattg tacttatacc
    55141 tattttcata tttattgaaa atagagctaa agaccctatt ttaagctttg aatatttttt
    55201 aaatccgaga atattgatag tacttatttt atctttaatt gttggtattg gaatgatggg
    55261 tatggtattc gttccacagt atgctgaaaa tgcattaaag ataaatgctg gttctggtgg
    55321 atattttgta gctatattgg gattatttgc aggtattgct gctcctttaa gcggaagact
    55381 tattgacaag tttggggcaa aaaaaatact attactagga ttttctgtat ctatgatagg
    55441 ctctctttac ctagttttaa ttgctctaaa aaccaatact gtatttagtg tatgtgtaag
    55501 tttaatattt atgggattag gaatgggatt tactatggga actcctctaa actatatgat
    55561 gttatcaaat acaagattag aagaatctaa ttcagcactt gcaacattat ccttgatacg
    55621 ctcaattggt acttcaattt ctccagctat aatgattgga tttatagcac atgcaggatt
    55681 atctgtgcaa gataatatta tggacttagt tggaaagccc tcaactccta aaatagttca
    55741 acttgaagaa cttgaaaaca tgatagatga cttaaaatca gaccctgaaa tggcaaaaaa
    55801 tttaaaaaat gtctctatcc caaatatgaa tgatacttca aatataaata tggacatgac
    55861 aggtggcaaa cttccaaaat ccttacttaa taaagttcag tcagcagata caaccaacat
    55921 taccaatata acaaaagaaa tatcaacaac aatgtttgat gaaaaagttc catctataat
    55981 tactaaaata cagaaaaatg tacaaaaagg aatagatgga acacaaagtg gaataaacgg
    56041 tattgaagat ggtgaaaata aactaaatag tggtattaaa ggagttcaaa ctggtattga
    56101 caatatggta aaagctagag ctggtattaa tcaaggaata gatggtgtaa aaaaaggtat
    56161 tgctggtgtt gacaaaggta taaaaggtat ggaacaaggt cttgaaaaac aagatgaggc
    56221 catcaaggaa cttacaactg cttataataa aatacctact acagaaaaaa ctcctactga
    56281 taacgaaaat aactcaaacg aacaaaacca gagtaatacc agtaattctg aatcagatga
    56341 cactaagaat gattctacag aaaataagaa taacaattcc agcgagaata aaccaaatgt
    56401 aaatgaacaa aaagaaagtt taaatgctca aatacagcaa ctaaaaaaga caagagatga
    56461 cttaaataca aagttacaaa aaaataaaac tcaaaagaac gaattaagta aaaaactaag
    56521 atcaatggaa tctcaaaaga aagagctaca aagcaagtta aataattcta taagccaaaa
    56581 gaaagacatg gaaaaaagcc ttgatacaat gcaacagcaa aaaaaatcac tccaagcagt
    56641 tttagcaaag acacaagagg taaaaactga aataccaaag gcttttgata aatcaaaatt
    56701 ggattatata aattccatag aaaataatag aacaaaaata gaaaacacac tacaatctac
    56761 tctaaactca ggatttaaac agatgtatat aacagtattc tgtgtaaatc tactagcatt
    56821 tataatatta ttgttctata aagaaaataa aactagataa aagagtgtta tagtattata
    56881 ttgtaaattt atcttattct taaatatttt attctaaaga ataaattcat aaaatttttg
    56941 taattgatta atataaatta caatataata ctatttttct cttaatcttt ttctctttta
    57001 cagacaatca aaaagcaatc ttagtttatc gaaataaatt tatacaatct attcatacac
    57061 ccctttacat ttatataaca attaaaccta ttgttatata tacaaatcaa aataaaccat
    57121 atattttcaa ctataatatg ttaaaatcat attatgagca tattaattta agcaaaatgt
    57181 catttagact ttaatttagt agttttatat tataaattta tatattttta attttaaatg
    57241 tcatttagac attactcata aaaagaaagg agtgtatttt gtggatactt taggaaaaag
    57301 aattgcttat ttgagaaact ccaaaaaact tactcagcgc aaacttatgg atattttaaa
    57361 atttgaaaac ttaggcaagt atgaaacagg agatagaaaa cctaattgtg atatactaat
    57421 gtcaatagct gattatttta atgttacgac tgactggctt ctctatggta aagagaaggt
    57481 aaatgtcaat agtagtgtaa aagaagataa agaagattac ttacatgtta ctaatgatga
    57541 aatgacgata ttaaatctat acagacaact aaacgaacgt gataaaataa aaatagaagg
    57601 aatacttgaa cttaagatat ctgaatataa agacttaaaa aagcattcct ctaataataa
    57661 tgaagataaa atggtttaaa catacataat ttgatacgat tttacatcat ctattaacct
    57721 tctttttttg tagtatatta ctaaaatatg gatattggat tatttaaaaa caagtatagt
    57781 ctaatttcaa gcacatttat aatatttaga ttatcttatc catattttag tttaagtact
    57841 ttgaaaaata taatacatct ctatgtataa cttatattcc taagaaatgc attactcatg
    57901 tttttaattc ttccaacatc tttctataca caattgtaca tccttcataa ttcccctctt
    57961 taagaaggat tgaacatgta ctagaatatt tagttaaagt atttaatctt tccatattta
    58021 agctttttat attactgagt gctgtaataa aatcatcaat tgctctacaa cactctataa
    58081 ccccatctga atccaattta cacacaagaa tattcaccta ctttatatac tatttattaa
    58141 tcatcttctg aatattctat attaagtaat tgataaattg atgatttata ttttttacct
    58201 gttcttcttc catttaaaat agctgacaaa tagttttcat taacacctat tttcttcgct
    58261 aattcccttt gagtcatatt taattcaata agtctttgct tgacttgcac tcccaaaaaa
    58321 ggtatccatt tttttcgagc atccatttat caacttcctt tctaagcgat ttatatttac
    58381 ttttatatta ttttagctta cgcaccccct ctatttagta tatattttta atatgtacac
    58441 atattaagta tataaattta tttaatacta aataagattt tttgattact ttgtgctaaa
    58501 attaggatgt aaattcgtat cattaagtta attataaagt cattttgacc ttaagtcaat
    58561 ggtttttatg tcattttgac ctttaatttg aaactattta catcttaaaa agtctttttg
    58621 acattaattg aatggagagt gtttatggat actttaggag aaagaattgt ttatttgaga
    58681 aaagcaaaaa accttaaaca atatgaacta gaagaaatgt tgggctgtga caacttaagt
    58741 aaatttgaga gaaacattag aaaaccaaat tatgaaatat taaaatctat agctgagata
    58801 tttaatgttt cagtagactg gctattaaat ggagataatc tatcacataa atcagattta
    58861 atttgcgatt cctcctcaaa ctatccactt aatagcataa actctaatga aataaagctt
    58921 ttaaacaatt ttagaaaatt aaatgattat gataaagcta aaatagaagg tatgattgaa
    58981 ttaaaacttc atgaatatga aaaagagaag gatttaggaa aaattgagta taacaaaaat
    59041 aaagatgaga aaatagataa ataattttta tgttgctttt gatacgattt tacatcattt
    59101 ctttgttcta atatttttat ttcatttatt ttctttttta taattttatg ataaaataat
    59161 tagtatattt tgctttactt gaatgtaatt atttatacaa aagtcaaaca taatataaat
    59221 gtagtaaatt tttagtttca agtaatatct cgttattata actaaatatt tattaaaatt
    59281 aggaggataa aatgggctta agagacaaat tcgcgcaatc atttgctaga tcaaaaacta
    59341 tgagtggccc tgagaaaaag gctaatgaaa taatgggtaa attgcttctt aagaaagcta
    59401 tattacctat cgttttaatg ttcgtgataa taatcgctgg agctatgctt aaaatcaatt
    59461 cttgggttac acttggcata aatttagtta ttgctgttgg tgctttcttc tacataagaa
    59521 attctagtaa aaagtatcag aactttaaac cttatgttgg caatttgatt agtttagaga
    59581 aaaaaggtaa aaaagagtac gttgctatta taaaacaagg taagttacca gttaagttac
    59641 aaatagctta tggtggtgaa gaccttgaac atgttaagaa aaatcaaatg gttcaaataa
    59701 gttataatcc agatgctaaa atagctattt tggttaatag acaataattt aataaaaaaa
    59761 aggagttgtc ttaaaattga ttttttctaa tcaaatttaa gctcaactcc ttttttaatt
    59821 tttatatgtt atttttatta ctttttttaa ttaatatttt aaatattaaa tccttctcaa
    59881 taaaatagtg aactatctac aatactactt tatcttttcc taacaatata taatctggtg
    59941 ctactctttc atgttataca ccattttgta acataccttt tatatcagta acatcttcct
    60001 taatttcctc tacatctgtt ttcattatac ccatttgaac aagtatattt ttgtttattt
    60061 cttgttgttg tgtagataat tctataaaat tttctacagt ttttttatac atgtctctat
    60121 cttctttttt ctcctgcata gtatttttaa ataatagagc acatagtata cctattgccc
    60181 ctagactact caattcggtt attaatttat ccattatagc ctcctattgt ctaaattttt
    60241 tatacttata gctttaattt ttgttgtatt cttacatcat gcaatattat attttcagga
    60301 ttatcaattt aatcttctgt actttaaatt gtaggtgtaa tttatagatt gaataaataa
    60361 taaatatata tacaagttct aatgtaacaa aaaataagta caaggcttac ttattaaagt
    60421 taagaccttg tactttacat aaaaaaaata actaatacat agttattcta aatttactat
    60481 tcaactggta tttctaattc tttacatcta attctaactt gctctctaat tactgctggt
    60541 acttcttcta ttgttttttt gcctcttaaa attaaagcta catatacttc taccattcca
    60601 ctctcacctc cagattttct tacactaaaa gtgcttatat tacttgcttc attgacagaa
    60661 ctttgattta acaacatctc atatatttct acatttgcta taaaattatt tgttatatca
    60721 tcatcttgct ttacttgacc tatacttatg tctgaaatgt ctttatactg catttctact
    60781 tttctacctt gtttgatttg ttctatttta tttttatcga atttaaacaa ttttcacacc
    60841 tccattttaa gcttgtggct cgttgttttc agcatcatat ctaaaaatca tagatggacg
    60901 ttttatagct gtccatttat ctcctttttc tactatgatt ttaggctgag cactttttat
    60961 gttggcttct tctaattcat agatttcttg gtaacttgag tctgattttt tgatttcaac
    61021 tagtttcatg tctttgaata agtcagattt atttattttt acttgaactt tagttttatt
    61081 tattaattga tatataggat tgttttccat catatcatcc tttaatccct catttaatgt
    61141 tactattaag tcagtgttat tttcagtaat gtttatgatt gaatatactt tattgtttat
    61201 cattaaaata gaatcctttt ttactttaag ccctgtttct gatttaaata taaacatact
    61261 tgaatataaa tcgcctgtag gactactggc tttaatcagt atcttatttt ctcctaatac
    61321 tattttattt tgtggtatct caaatataat gctatttatt tccaaattag tcttagtaga
    61381 tattaattca tcatttataa acatttcaag tttttctata acattattag ggtcttgtac
    61441 tacaactctc acttgagata ttgatttttc aactatttct atagatgctt tacttgtatc
    61501 tacttttgtt atcatgtata ttgtatcatc tgaaagctct atatctaaaa tatcatttag
    61561 atgttcagga attggtattt tagtaaattc atataaatca gtaattgata tagatatttt
    61621 atacatagat ttatttttac cagttatcca tagactatta tcatttttaa ttactagtat
    61681 gtggtcatca ttatgtgact taatttcttt aacattagtt aatactttag taaaagttct
    61741 tattatctta tctttttcac tttctcctag tccaaataat ccatttgtat tgtacccttg
    61801 agcgtacaac tctccctcat tagttaaaac aagtgtcatt gagttatata tacttatatc
    61861 tttaacagaa ggtaatccag taaccttttg taactctctt acatcattat tgtggccaac
    61921 tcccaattgg tactcagtat tctttccata tgaccataga gtattatctg tcttaataat
    61981 ataggagtag ttatctccac aagcaacttt tttgacatct gatatattta cttcagtaag
    62041 agttaataat tctatatctt caagggtttg attaatttct ttatatgaat tacttcctat
    62101 tccatataac ttgccatcct tagcaacagc caatgaatgt gtttttgata tatcaatatc
    62161 agatacctca aatggcaata ttattgaagt atacttaaaa ttatctttaa atatgttttt
    62221 ataatttata tacttattat ttgtttttat atacttatca ttaaacatta ctagcttttc
    62281 atttttctta ttaaaaacta ttttatctga tgtgttatct gttttaaaat atgaatgtag
    62341 ggaaccttca agagcttcat ttttatcatt atcatatcta tggtgaatac ctaaatgatt
    62401 atatctatta tctcctgttg aagaataaaa ttttccatat atatctttaa taaatatagt
    62461 attgtaacta gaaaatacag tatcaacatc tttctttata gatactttat aatatggaac
    62521 tgacatatcc ctataagatt catcagatat accataagta tctctacctg atgatatcaa
    62581 attgttatta ttatctatat aaagtgtatt attagctgtt acaaaaacat ctttaacatc
    62641 ttccacacta tttgtattat tattatttgc tatacttcca tatccactaa gacctccaac
    62701 aaatagttta ttgtcatttg ttaaaatggc tgaagtatat ccattaacta ctattttttt
    62761 tgcatttata atattattaa ttactcgtat cccctttata tcattagtct ctgttgaaaa
    62821 gaataccctc tcttttttcc ctgtagtata aatcatattg tcatttgtta taaaaaatgt
    62881 agcttcactt cctgctgtca caacagattt tacattctca atatttattt ttgtaaatgt
    62941 acttatactt ccttcagtaa acccaaaata gccatttaca agtccacata catagcatgt
    63001 attatcattt ttaacaatga ttgatacatc attacataga tatacatctc taacaccatt
    63061 tataggtata cgtgtaaata tattcctact tgtagtatca cccaatccta actgtccttt
    63121 tccattatta cctgctccat ataaaagacc atcattagtt aataatagag atgtgtttcc
    63181 atatacagct acatatttta cattttcaac atttatttta gtaaatatat ttctatttac
    63241 attatctcct attcctaact gaccagaatt atttaaacca acagcataac aggtattatc
    63301 attttttatc gcaaatgcat gtttactatt gtcagtaaat ctatttacat ttatagattt
    63361 tatattatca atatttattt tagtaaatac ctttctattt atctcatctc ccactcctaa
    63421 ttgacctaca tcatttaaac caacactgta aagcatatta ttattagtta ataaatatgt
    63481 agactttgaa tggctatata tttcttgtat attagaagat atttcactac tgctattctt
    63541 tttattgtaa tttatcttat tatttaaatt taaactacta ttaactgata taccatgcgt
    63601 acccttagag ttatatccac atataaataa gctcttatca gttaatattg caaatctagt
    63661 tttatctcta cttaaatcat aaggaggtat aaatatattt tttatatttt tcattacctt
    63721 tttataatta gtggcattat atccatcttg tgtatctgaa acatctagtt cttttgcact
    63781 ttcagatata ttcccaaaac catatagcgt gttagaagat aaatacaata gagctttata
    63841 taaaaatatg gctttatcag aggtaatagg acctgaattt atattagatg ctgcaatatt
    63901 attagatgat tgattgttaa gataattcct tccatattga tgtatataag atgaacctcc
    63961 agaaataagt aaattatctg gattgacatt ttttaaatta cttgacgatt ccccattata
    64021 taaataagca aaagttgtag aagatgatga ttgtgtacat cttgtaggat aataattata
    64081 tatataagaa tcaagatatc ctgcatatga aattccctga tattgaactc tatagcttga
    64141 atcagttggt tttgtccatg aagtaccatg gtatccagtt agaaaagatc caatgtttgt
    64201 acaaaataca gttacatact catatatgat aatattatta attttagtaa tattattcgt
    64261 gcatgaaact ctactataac ttgaccctga tatatttata taataccaat cacttaataa
    64321 acctagattt acattagatc catcagaagt actaaaacct aacaggtaat ttgtatttat
    64381 accagttgca tatacctttt tttcatcaac tacaataaat gttgcattat atgaactata
    64441 agcatcagtt acattactaa ggctaatctc agtatattga cttattaatg cattactaag
    64501 tccattaaca taacttgaac tctttccaga agtgtatatt tgcttattat tagttacaat
    64561 taatgaaaac cagtttgata aatcagtatt tataacaact ttttttacat tatctataga
    64621 tattttctta aatacatctg aattagaagg aagaacacct gctattgagt tactatttgg
    64681 tccagaataa taacaagtat catcattttt aatgtagaat attcctccag aaatattttt
    64741 tgatgctact atagacttta cattagagat acttaatttt gtcaactcat ttaattgact
    64801 tggtaagtca gaacccattc ctttgtatct attatttcct agaccatata catctccatt
    64861 ccctaaaata accagagtac aataaggact aaaaacgaca tctcttacag aatgtatatc
    64921 tagtggaagt tcaatatcct ttatttccat ttcttctttt aataaagtta atttatcctc
    64981 agatttaatt aaatttatat aattactata actatatttt tcactatcca ttttcttaaa
    65041 ttctacaatg tgcatatcat caaaaaacga acctaattgt ccataagtat tatctcctgt
    65101 acaatatgaa tcattatttg aatcaattag tatgctatgt gataaaccaa tgaaaatatt
    65161 cttagctaca atatttttta aatctataaa tgaattatct atattctcag agtaatttgc
    65221 tatacctaac tgatatatat cattatttcc agcaaccttt aaagtacctt tgttatctat
    65281 aagcatagtg ttaccatgac cacatgcaat agtttttata tttacttctt ctatcttagt
    65341 aaatagtgat gattgaagtg catttccaaa acatttttcc tttccacaac catatatatc
    65401 attattatac gtcattaaca cagtgtgttc agaaccacaa gctatagtct taacatcatt
    65461 aatagaaact aaactaaata aatctctatt attcttatct cctaaaccta gttgaccttg
    65521 attattaaaa ccagtagaat agcatttatt gtcattcttt attataaaag ctgatttatt
    65581 atatgaaaat acagccttta cattgtcaac atctactttt gtaaatgtat ttacatttgc
    65641 agatgctcca ttagttccta gacctaattg accatacaaa ttatttccag caacatataa
    65701 agttccatca ttgtataaaa gatatgttga ttgaagtcca catgcaatat cctttacgcc
    65761 ttttatttct ggaaccttct caaatgtaga aactgttttc cctacttttt ctaatcccaa
    65821 tcccatttga ccatctgtat ttatacctgc gacaagtaat tctccatctg aagtaagtat
    65881 aactgcatga gattctccac atgcaacttt tttaatattg gtagatatat ttggtatttt
    65941 cgtaaataat tttcttgttg tattgtcttc tcccaatcca agttgaaagt tactattatc
    66001 tccacatcca tacaagatat tattattttt tagaccaaat gtatagttac aacatccagt
    66061 aaaaaaatta ataaagtcat aattatcata cccttcatca aattcatcaa ctaccatatt
    66121 tttatcgcca aagaaattac ctctttgaag tagtttagtt cttttcatta taaaaacctc
    66181 ctaattatct tatttcatac gaatatttat ttaaagttat atctgtctga ccaattgaat
    66241 atgccctatt agattttgtt tcaacaaagt tattatccat ttcatgtata tatctaactc
    66301 tatttgcatt aaatggtagc accacattct taaatgtaac actaccaagt tctccagtta
    66361 tacactttct atttaaatct aacttaaggc cttctatagc tattatgttt ttagcattag
    66421 ttaaggtatc ataccaatat ccagcttcat ctacatctat ttctgactct aattctaagt
    66481 atattaagat atctaatact ttttcagata atttattgta actatttttt aatacagata
    66541 gttcagtttg tatttgacct cctattacat catcaataga tatattttta attttttcta
    66601 ctacctcatt tatagctcct gataagtttt tagcttctgt agataagagt tcttcgccaa
    66661 tttttacttt tatttctttt atatttttat caaacttatc atctaaatct tgaatatcct
    66721 tttgagttac aaaaaccaac gtcgggtcta cttctaatgt aacatttgaa gtattggaca
    66781 attgtaaaat catttttaca accaaatctt taatactgcc atcttcagca cgtggcttat
    66841 acgtctctgg atacttacct atagctatta tattattctc attatccaga acagcagctt
    66901 ctcttatcat aaatccacca actgttcctg gtaaaatagt ctctataact atccaattag
    66961 gatttttttc atcaattctt agagaattta tatttccttc ccaaactaca ttctttaaag
    67021 cagtttgttc ctctgttggc tcataatatt ctccattact atctccaact tgaaacttgg
    67081 ttaatccaac aagctctcct agtgcagttg catttgctat tttttctcta ccaattttgg
    67141 ttaatattat ataaaatttt tcttccaatt ataccacctc cgatttagga taaactacta
    67201 cattttctac acctgtatca tttgcaatag ctatattaat cttacaatta ttttcaatgc
    67261 atttaatgct taaaggataa acctttattt cttctctact tatcattcca gctccacagt
    67321 ttacaacagc actacatttt ataattggtt caaagtatgg atgaactttt acctcttcac
    67381 tacataccat acctccacca caatagacca tacttttatc tagtattatt ggttctaaat
    67441 agtgtaataa gtgagcaggt tttactcttt caatcatatc gctacaatct aaaagagttt
    67501 tatagtcaca gttatttgct ataaaactca atacgaatgt aaattcatca ctataaactt
    67561 ttatatctgt ttctgatttt gtatatgcct cacatatact ttttataact tcaatagtac
    67621 tagtacctct gcttctcatt ttagctttta tattgctacg tcttgtgtca aaatcaagtt
    67681 cttttttttc aatgcagagt atgtcttccc acatatctaa tccccatgtt gcagtgtcaa
    67741 caaataattg gttaaaggta tcatcaatat cttctttaag aatatttaat tctgtatcgt
    67801 atgcaccttg tatctcctct acaatgtaat ttctatcaaa tgatggtagt ttatcaatta
    67861 gcttcattga ttttccacct cactaaaatt aacagttgtt acactaggta ttttatcttc
    67921 attaatagtt atattatctg tacttccatt tataagtagg ttacttaaat cgtgtacacc
    67981 agtagtattt ataagtattc ccattacttt tgtatagatt atctctcctc taatatctct
    68041 aaagtatgta tttatacttt ctaggaaaga ttcttttaca ttgtctaatg tatatccatc
    68101 ttctagtttc attactgcac ttatacttat ttctattggt aatggtgtca caactgttat
    68161 agttggtcca ataggcttct cttcatctat atgttgctga cacctttcaa ttgtttctgt
    68221 atcaacagct tgattatttt tcccaaatat caagacctta actgttcccg gaccatccca
    68281 tcttggataa accttaacat tatagactcc atctacttcc aaagcccatt cttcatagtg
    68341 agctttattt ccacttgtag cttgatttct ttggatttta taaaatcttt cttttagttc
    68401 ttcatctgtc tctatatctg taccaccttc aaaacttctt gtgttagtaa tctttgttac
    68461 tccagatata ttatcaacta gtttaaattc acaatttgta ggtaaattat atttcttacc
    68521 aacttccaga gcttgaactg ggctattgtc accttcttca ctaccaatag ttacatcttt
    68581 tattactaca aatagtaaat ctctatatga tattattgtg ccattattta ttacagtacc
    68641 tttctctcca ataaattcaa cttctccatt tgactctgta cctaactttc tatatacacc
    68701 aaattcattg actcttttat caagaaactg gttatatgtg tcttgtataa aagccatttt
    68761 atgcatcttt gaaagttcta gatatatctt cgaaagttct agattatttc cagataccat
    68821 gttgtttaga aaagaacctt ctcctttata aatatcaaga ttaatatttt caagagttct
    68881 attttttatt acttcatatg tctggtcact atacatttat ttccacctcc ccatagatag
    68941 tggatacttt tacatttgca ctcaatatgt ctctgttaaa gttagcactt tttacgttta
    69001 cttctaatat atatggattt attagaaggg cctcttttat gaatctacta gcttcacttt
    69061 ctgtaagacc ttttgtatat ttttgtccta ttagttctga aagctctgtc ccatattcta
    69121 aagagtatat ctcatgttca tatctatttg tctttataca cttgtatatc caaactttta
    69181 aggcttcttt tttttcaatt attttaaagt cacctttttc taaaataggt tcatcttttt
    69241 caaaatccca tgccacttca cgaaagattg gcaattcttc tgttttaggt aagatataat
    69301 cctctgggac acctataaaa ggaaatattg tactcattat aagctcacca acttacttac
    69361 aacagcaaat ttttcgccta ttctaaacat tattaccata tccccagatt caaagttatc
    69421 tataaaagga ttttttactt catgctggtg ttcttgattt gtttctgtgt caaatgtttc
    69481 tagctgtcta tcaatcatcc aactatctat caaaatatct tctttttcta atatgatgtt
    69541 atttgtctct atttttaaat ctggtaattt acttttaatt tttccaataa aaaaagaagg
    69601 ttgattatga tactttcctt cttctcttat tattcctata aattcattta ttggattagc
    69661 cattatatca ccatcctttt ataatactct cctagctgtg ttaaaatctc ttctactact
    69721 taacttactt attttaacta catcaccttt ttgtggagca tgtaaaaatt ctccattacc
    69781 tatatataat ccaacatggc tgactggatt atgaaaaaat actaaatctc ctgcttgaag
    69841 atttttttgt tctactttct tgcctttttt agattgttga tttgacgttc ttggcaaact
    69901 aacattaact tttttaaaac aatagaccat taaaccagaa cagtcaaaac tacttggtcc
    69961 atttccaccc catttataag gtttacctag atgttttttt gcttctgata ttactatttt
    70021 agctttatca gatacattac cagaataacc accaataata atttttcctt ttcttcttcc
    70081 aaatttacta gcttcttcaa catttccaaa taaaatatct atatggtatg ttccattctt
    70141 ttctatagtt attgcaggtc ctacgtcatt tactttatag actccatcac gaccagaaac
    70201 tcctgtaatt acctgtattt catctccata gtttaataaa ggatgtttat ttaaaaactc
    70261 ttttgtataa taagtttgct catatttacc aaccatagga gcagcgcaag tttttttaga
    70321 tgggtcaagt tttttctttc tacaatctgt atctccacct tcttcttttc taggacagta
    70381 agctgtaaat tctgctgtaa actcttttcc tcctgcataa tcttctcccc ctaaattact
    70441 ttcttcctta ggttcatcct gtcctgctga cttttcatcc ataagatttt gaaaattaag
    70501 ttcaagctca atttgatatt ctccattttg ccaagtatgt ttgtctgtat ctatataaaa
    70561 tagtcctaca agctttgtat aagaatcttt aacttttact cctctaccag ttatacaact
    70621 tacatctcca taacctttaa gagaacagct tttttctatc ccattaaact cgctatcaat
    70681 atctacatct tgattttctt gttgctgaat tactttttgc attattacat ttacttcctt
    70741 aaaaatttca ttatctattt tttcgctaat cttgcttcca tactggtcta ccactattac
    70801 tttatttttt acattttcca tgctctccga aaaggtggta tttataatat taaatccctc
    70861 ttcaaacata acacttaatg taacagttcc tttttcaata acattaaact tatctaaatt
    70921 ggcctctatc atatactttt tctttgtctt tttacttgct tctgtatatg cactcattat
    70981 tgtgtcataa ccatttactc ctataaacat ctttgtatac ttgacattgg tctttgctat
    71041 tattccaact gaaagcctat tttcagcaaa tacttgcttt gctatgtctt caactaactt
    71101 atctttaaaa ttgtatgaca cttcactttg tgtaagtaaa aatcccatat ctttagatac
    71161 aaaactaatt tcattactgc ttgaatcttt agacctatta attatcattc ctctaaagag
    71221 ttctttctca tctacataga aacagactgt actagctatt ggtatatcga tttgttggaa
    71281 atttacatca aatgatgatt gtattattga aaactctagt gtccttgatg gagatttata
    71341 atcacctgac catgatacct tgtctactat gtcagttatg tcatatatac ttccgttttt
    71401 tatgtgtacc caaatcttaa taataaaaaa tcacctgcct tttcatttaa ttctatcagc
    71461 agttatttat aacttattat ctataatagt tattgttttg gagttacttt tggtatcttt
    71521 attcttttat actcttttaa atctaatgta aaatatacat ctcgagtccc atctctttct
    71581 tcataactaa acccttcaat tatgacttcc atatttatat ttgtttccgt aattgtaaat
    71641 cttaatataa aaccttcctt catccatttt tctatcttat ttacacaatc atatggtgat
    71701 ggaaaacctg tatagtcaca gaaagtatag tctttatttg gaaaaaaact tgatattgaa
    71761 atgcttttaa gagctacacc accaaaagtt gctatttcac ctattttgag tatactagaa
    71821 gagtttacag cagcttttcc attaatacta aaggaagatg gaaatactgg aaatctaaat
    71881 ctatcttcag cttgtctaag ccacatttcc attataagac accccctaac tgtgccaatt
    71941 ggatacttgc aactaatttt gatgttatct tttctatatc agcttcttct ctaatcacta
    72001 ttgtatcagc taacttagct atgtttatat ttccaccatt ttttctgctt ctatattgat
    72061 ttgcttcttg tttagttaaa accatttcac cttcatggag tctggtttga tagtcattat
    72121 aaggaacata gttcaatcca aatgcattat tacctttctt acctgaataa gctttaccag
    72181 aatcaccagc ctttgttcca gcatatggat tatctttgta atttttttta ctcttttcca
    72241 ctagatttaa aactgttcct gtgatgcttc cattttttat cttattaatc atagtttgcc
    72301 accatttaca tatatcttta actattttac atactttatc taatatgtta aaaaaagctc
    72361 ccaatattgg ctctataatt ttccatccag tttccaacag aggtcctaag gtctcccata
    72421 cagttttcca aataacaccc agtgtctcta taaaattgtt tatttgaggt gcatattcac
    72481 ctaaaaaagt aaaaattctt gtgataatag gactaacagc ttcaactaca ggtccgatta
    72541 gttggaatgc tgtagttact attgtaccaa atatacttat taatccttgt gcaattggtg
    72601 atttattgtt tatactatct agtaaattat ttactaagtt tactactgga agtaatgaat
    72661 ctattaatgc tgtaaaatcg atattttgta atagtgtact tcctgcgtta acaattgctt
    72721 ttggtgcatt ccctatcatt gttgctgcat gattttcaaa acgttcttgc ttattttcaa
    72781 ccatcatttt acgcttttca gcctcattag aaattcgata ttgagtagaa tcagaaaact
    72841 cttttccatt ttttacactt ctagattgca tctggtcata tttgttgcca ataattatct
    72901 taccattcat tacattgcct attttaccag ccattttttc agataagcca gctaaattgc
    72961 ctaatccact ttctataaaa gtttttattt tctttagtcc ttgacctaaa atatcatcaa
    73021 aaggtttaac aatactcttc aagccattgt taatgcttgt ttgagctcct gatattatac
    73081 ctggtattgt attactcatt tctttagcta accccatctg ctttacactt atgccttcca
    73141 atgcttcatc aaaagtttta taattttctc ctaccagagt attaagagat tccatgtttc
    73201 cttttgctgc acttaagaaa gctgctgata catcttgttc actacttgta ttcatattta
    73261 aagctctaac atctaccata gcttgagtta tatctttttc ttttttgcca ccagtaatat
    73321 tactaatcat tttagcttta gttcccattg caacaacatc ttcagggtca aaaggcgtat
    73381 catttgccat tcttactaaa ctgccataat aatcatcaac atctatttga ttaacttttc
    73441 cagtatttct tttcattaca ctttctagaa caactttctg attttgttcc tgaccaagta
    73501 aatcaacacc ctttttcaca gcgacaccta tagtagcacc atttgtcaaa tcagatatct
    73561 tcccttttat cgaaccaagt attccacttg ccatatcctt tacagctatt gcaccactcc
    73621 ataccttacc tgcaaataat ctcaatttgg gaaatacttt acttataatg ccacttgcca
    73681 tatcttttat agctattaca ccactccaaa aagtattttt aaacaacatt aacttaggca
    73741 aaattctacc catagcacta ctcaccatat ctttaagatt aatacttcct tgataaggac
    73801 gtttctctac ttgttttaat tttcccaaga tactacttat cactctctta gtcatatctt
    73861 ttgcttttat tacgatattc caaatcttgc caccaatctt ttttaaattt ccaagtaatt
    73921 tatttttagt tttttctact tcatcacttt tagctttaaa tattaatata atcataatat
    73981 ttgaattaaa ctcatttttc ttttgaatca acttattaaa ataatcaata atcatagcta
    74041 tagttattgc tagtctcata atacttccat ttaaacttcc aataactcct gaaacagcat
    74101 ttatgctaga acttaatttt gtcatagttg aacttacact actagcagta gtttgaactt
    74161 ttcttaacga ggaatttaaa ctgtttactc tattacttgc acttccaata gaatttttta
    74221 tgctactagc cattacatta gtggaaagtg aaacagccac agttgctctc ctaaaattat
    74281 cagttactga tataatttga ttcatttttg gtgtgtaatt atctgtaagg tacatattta
    74341 taccttcttc tctcacattt cccattacat accacctcct gtcaaaggac acactccata
    74401 tccatgtttc attttttcaa cttcttcctc aatttctaag gcgaaaaaag cttcaataag
    74461 ctttaattcg cctttatcca ttgcataaaa aagggacggt cttatacctt ttttcttcca
    74521 ataatagtac attattgtag tcctggtatc cgtccctatt agttttttac ctcttctatc
    74581 actgcatttt taccataacc cataacatct tgaagtattc tatataagtt ggtaatttca
    74641 cctggtaata ataattttct tgctaattct tttggtgttg gagccttaaa tttatccatt
    74701 agctccttat ttttaaataa aagctttcca tccaaatcac aaacaccttc tataattgta
    74761 agcatctgca tctccggtaa gtctatatcc gttttcatat tttcatcaat tttaagacaa
    74821 gtattttgta tttcttcatc tttacttatt gttaaagctc tacacattac tttaaattcc
    74881 tgcccaaata tagttgataa tctcttaagt tcaactatag tacttggtct ttctaagttt
    74941 tctgtatctg aatttaaaag taaatctact atgtttatat tttttgataa tccattttca
    75001 ctcattatta tttgctcctt tatcaatcaa attcaatcaa ctaacttttt tacttattaa
    75061 ctatctgaat ttattaagta tatacttact aaatatcagt ataaaactaa atataaaact
    75121 aaattatatc aagaaaatca tactcagtaa atgtgaaagg agcttctatc tctcctttta
    75181 ctccaacctc ccaatcagct aaagttaaat catcaaatgc tatatttttt actgctattc
    75241 tttctgctcc ataagaatct gggtcattta attttgatat agcaacaaat ctaggttcag
    75301 aacctctctt tatcttttct ccaattaact tgtgcattct tgaactaacg tggtatagag
    75361 ttattgagcc ttttccttta tatcccatat actttgtatc agtacccatt tgacctgcta
    75421 ttataatatc ttcttttgta aattccatct ttgcttgaaa tttctttact tctgctactt
    75481 tgtttccatc aagccaaagt tctccccaag taccactcat tacatttcta gcttccatat
    75541 ttgccatatt aataatcctc ctatatttct attgataaat ctatatcttc catagcatca
    75601 agtactttta tttttgcttt taaaaatact ttagaacctg tgttagcttc ttttatttct
    75661 tgtaatgtca tataagataa atctactcct tttgatttta aatacgattt ttgtgcttca
    75721 aaatctattt caacagtaga atcagattct ataagtgctg atttttctaa ttcttctaaa
    75781 taacttttta tagctactat caataaacat ttgttgtcat aactgttagt aacctttcct
    75841 atatagtcat ctattatcac ctttcttatg tcactatgta taatatctaa agtgtcaact
    75901 atttttattt tctggaacat ttctcctttt tctgctgtta attcagttaa agaatttact
    75961 cctctagcaa ttcttatagc tcctgcttct ttaataagta taagctctcc tttattaacc
    76021 cttgattctg catcaacttt cgtcatctta ggtatatcga ctacatcact aagtttagta
    76081 taagttactg attgacttaa aggtgtacct gctataagtc cagccaccct gcttgtaaac
    76141 tcatcaacac tgtatttctt ttctccaact aacacatctt ctgtagtaaa attaattatc
    76201 ccttcatgat ttcctacaac ttttcctaat acagctttaa ccttaacctt atctatatct
    76261 ctaagtttaa ttatccaatt ttttatagca gtcttatcag cttctactgc ttttggcata
    76321 catagataat taaattcctt agtctctaaa aaatctaatg cagtttgaat atctgcttct
    76381 ccttctatta cataaactaa taatttattt ggagtgttaa catttcccat caaagctaaa
    76441 tttatatatt ctttattttc agcagataaa tcaactggta tatcctcttt ttcatgtatt
    76501 tcattaagac ctagtgcctt agcatcttta agtaccattg caattattcc tctagctgaa
    76561 cgttctttaa cagttgtagc tagctcctta aatgatatgt tgatacttgg taatcctata
    76621 gccatactat cactctccta aatttaaatt gatttctttc atattttcat atactacatc
    76681 ttcttcatat acactatcca gatagtttag tgtaaatgta aagttcaata cataaattga
    76741 ctcttctttt ttaatacttt gttctagctt tgttatattt aattttctgt ctgttacctt
    76801 tatatttctg ttaaatagct tatttaattc atctgaaatt tcatacattc taatactctt
    76861 tgactgtggt tttggtaagt attttataga gataacaata tctttgttat tcgtatttaa
    76921 cattacaggt gtacaaataa ctgatagtat acttacaaaa aaacaagacc cttcagagcc
    76981 ttgcacactt tcatcatcaa taaaaatact ttcagtaaag ttttttttga gaatctcaat
    77041 aattgtttct aatatttcct tgtatttaag caaataatca ccacactatc actaaatatt
    77101 cttgcttaat ctgataatct tactataaat gatataagtc atttttaaat atatatctca
    77161 tttattcctg tcaagtatct tctatatttt taggtaatat tattcttttc aatctatcac
    77221 ctccttcaat atggatttct ttcacaataa cattatctca catattttat tgcctattgt
    77281 tgcaatggtg tatataaagt gttgtaaaat tgtcctattt tttataatat ctcactcaat
    77341 ttaaatatca tctttttctt taaattgtag caagatgttc tatctaagtg tagtttcata
    77401 gaaatatatg tcatattgtt ttttgtctta ctattataaa aaagattaaa aaattccatt
    77461 tctatatcat ttagacacgt tagagcattt tctatcttct ctttttctat ttctatatct
    77521 tctttttcca tttttaattt tcttaatctt tcctctcttt ttaacacctc attttccaca
    77581 gaagagctta tgttatatgt gttactagtc ttttctgtat ataccattgc tccacatcca
    77641 gagtattcat tctcacatct ttttatgtcc aagttaatat tctttatctt tatatccaaa
    77701 tacttataat tatgtaatct tttatctgtt tctttaaata atatatcttt tttgttttcc
    77761 atagtacttc ccctttttta tatacttatt attagcttca aatttacaat aatctcttta
    77821 gtgcacagtt ttttactaat ctcacaactt tcaagaacat attgttctat aactgtataa
    77881 aaaaaacttc ttctatattt tgttctaaaa tactagaaat cttcttagcc ataagaagtc
    77941 caggattttt tatacctctt tctatttgag atatataatc atttgtaatt ccagcctttc
    78001 tggcaagttg aatttgtgtc atacctttgc attttctata atatttcaag ttatttttaa
    78061 acattgtttt atacctccat tactattaag aatattttgt tcttcctcac ttagattata
    78121 tagaattatt tgttctttat caatagattt tagaaaaaaa tattctttta tattttatta
    78181 gttttatttt ttagaacaat ttgttataat ctatacataa ggtggtgtta taatgtttgc
    78241 caaaagatta agagaactta gaaaggaatt tggattgact caaagagaac ttggagaaaa
    78301 agtaggcgtt tcccaaagag tcttagggta ctatgaaaca gaaaacagat ttcctgatga
    78361 acacatttta aataaattgg ctgatgtatt taatgtatca gttgattacc ttcttggaag
    78421 aaccttagtt aaggaaaata ttgatacagt agctgcacat agaaaaaatc cacatgaaga
    78481 gttacctgaa gaagcacaag aacaacttaa tgattatata gaatttttac taaataaata
    78541 taaaaaaaaa taaaccatat gatgagcagt ctatttttct gctcttttaa tataatttaa
    78601 aggcaaacat acattctatt ttagggggat ttttatgaat aatttggata agctttttga
    78661 attagcttct caagaagaga taataattca ttataccact tatattgcag gtgatttaga
    78721 aggactatat ataaacaaac atggtattaa aattatatca ttactcagta atttaaaaca
    78781 aaactcaaaa aaattgacat ccatcttagc tgaagaactt ggacatcatt tcactagcct
    78841 tggatactat gtatcctctt ataacgatta ttatacaaaa attattatag ataagtgcga
    78901 aaataaagcc ttaaaatggg cttgtgagtt cttaattact gaagaagata taataaacat
    78961 aattaactct ggcatcactt gcgtttatga aatggcagac atacttaatg tggatattac
    79021 tttctttcaa aaaagattag aatttctatc attaaaaaaa cagtctttac aacttggaaa
    79081 taataaatat ttaatattaa ctaatttgcc gtatttctac atatttgatc caatttcata
    79141 ataaaaatag tagtaatttt aactggctta acagtaaaat ctatatattt attttaacaa
    79201 attttggttt ctataaattc aatattaata tataataatg atatcatatt atttaaaatt
    79261 ctaaaaacaa atatatgctt ttgttaagaa aggttgtttg atgaatgtat aaagaaaagt
    79321 tgtttgataa ttattttaag ttcttagcac tattattttg gccaattatg tggtataagt
    79381 ggatagtaat ttcaaatggt actcttgaaa atatgttatt taccatatat gcaatcgtcg
    79441 caattgtttt catcatttta tattcggtgt ttatgataaa atataaagac attacacaaa
    79501 tagatttctt ttacaggata agtactctac ttgcttttat atttacactt tttagctttt
    79561 taatataccc aaagagttta tttttcttgt atttaaagat tatttttact ggaatatacc
    79621 tttattattc aatagtaaaa actcttaaat ttaaagatga cgaaggtgta gtgggtatta
    79681 tgagctcact tttattaatt gtaataactt tgttctacta aatttaattc tatttgactt
    79741 aatatggctt taaatattag acatctacat tattttctga tattatttaa gatggtagtc
    79801 ttatattaat aatgaaatac actatgaagg agttaactat tatggaaaaa aaagttttag
    79861 caacagttgg agaaaaagag ataactaata ttgatattga aaatgcatta aaatcattag
    79921 acccttatca agctatgcaa tttaaaactg aagaaggaaa aaaacactta ttaaatgatt
    79981 tagtgaatca agaattattc ttcttagatg ctaaagaaga aaaattagat gaagaagaaa
    80041 tatttaaatt agaaatgaaa aagatagaag aaaatgtatt aaagcaattt gctataaata
    80101 aagttttatc tagtgttaat gtaacagaag atgaaaaagt aaaattcttt gaagctaata
    80161 agagctcttt ctctaaacca gaaagtgcaa ctgctaaaca tatattagta gatagtgatg
    80221 aaaaagctaa agaaatactt gctcaaataa aatcagaaga aatatctttt gaagatgcag
    80281 ctcttaaaca ttcttcttgc ccatctaaag atatgggtgg tgatttagga acattcggta
    80341 gaggacaaat ggttcctgaa tttgaagaag ctgtgttctc tatggctaaa ggtgaagtaa
    80401 gtgaaccagt taaaactcaa tttggatacc atataataaa attagaagac cttcaagaaa
    80461 gtacagagtc tacatttgat gaagttaagg ctgaagttga aaaaagttta ttatatcaaa
    80521 aacaaaatga agtttatgga aataagataa atgctcttaa tgctaagtac ggaaaccttg
    80581 taaaatacaa cgattaatca taaggtcata tataaaaaag ctgtcttttc acaatattta
    80641 ttagaaaaga cagcttttta tattaaagtt taaatattca gtttcatatt atcattcttt
    80701 tattgtaaag aactcagctt aaaagtttaa ataagatttt taattatcat tagctaaatt
    80761 tttatatcca cgtattatct cataacagta acctattaca tctcttcttt ttattatccc
    80821 aataaatgtg tcttggtcat ctataacagg aatgaaattt tgatttaaag attttataac
    80881 taaatcttca atatctgcgt tgatggaaac aggagaatta tccatttttc tatttatatc
    80941 tgtcactgga acatcttcca atccctttaa gtccagattt aaatcatttt ttaatgtcca
    81001 taaaaaatca ccttctgtta tagttccaac atatttacca tccttactta ttattggaat
    81061 tgcagagtat ttgtgatatt ccattttttc tagggcttgc cttatagtgt agtcttcata
    81121 tatatatgct acttcacttt ttggtgttag aaagaataat atgttcatta cactacccac
    81181 cgttactgaa aacttataaa tattactcaa tatttataat gttacttttt tgcagtaact
    81241 ttctcctttc ttattttcta agtattctta atactatacg ctgtaacaag cgtttttcct
    81301 ttgctcattt taatcttatt atgctttatt aatattttta tatggtctat cagtatcact
    81361 tattctcttt ttagaaaatt tactaaaaat taattgactt aaacttagcc ataataattt
    81421 tactctacac tattaatttt aacatattta tctcattatt aatgatttta ttttaataat
    81481 tttaatttat tttgctatta attttattta caaaaactga aaaaaccgct aaatgattag
    81541 cggcttatct ctcatattac tagaacacta atatatatga atttttaatt ataatctaac
    81601 tacgttagca gcttgaggac ctttagctcc atctactact tcgaactcaa cgctttgtcc
    81661 ttcttctaat gttttgtatc catctccagt tatagctgag aaatgtacga aaacatctcc
    81721 ttcaccttct acagatatga atccaaatcc tttttcgttg ttaaaccatt taactacacc
    81781 agttttcatt aataaatcct cctaaaaaaa cacaaatatt tagctactct tttctgtaac
    81841 cttattacta ctctatcata tatagctatg tttgtcaatt attattattt ttatacatta
    81901 ataatcaatt tttttacttt ataattttaa atgattatta tcttttacac tttatttttc
    81961 tttataattt tttaataatt tatttttatt atatggaatc ttatggactt atcactccaa
    82021 cctaaatccc atggcaccaa cattctatca gtattatgac aagttaccaa aggataccct
    82081 cttgagtcta atcctgtcac tgtcgaaaca tgtgttattc tgcctttctt ctcataacca
    82141 acaaaatccc ctggacgtaa attataagct tctttgtata cttcttcata ggaaccttta
    82201 gcaattaatg aacctcttcc actatacagt gcataatttt taaaagcttg tgcatttacc
    82261 catgcttttg tacctgctct ctcgctataa ttccaaattg agttcttttt gaaccttcca
    82321 ctttcaaaca ttatctgtga tgcaaagttt gcacaatctc caccttctgg attataatcc
    82381 ctatagtttg gattaaattt cataccatgt tcttcatcag ctgcggctcc acagtattta
    82441 tgtgcataat ctatagcctt tttttgttca tctgtaagct gtaactctac accatcttgt
    82501 tgaccaatat aatttcttat atcatcagat tttatatttt ctaaatttaa agagtctgca
    82561 aaagggtctg tataccactc tttagttatg acatattgat tatctataat ttttacattt
    82621 agataatgat atgtacctat tctaaaggca ttttctttgt ccttttgatt ttcatatgaa
    82681 tacttatatt cagtcgatac tgaacagatt ataccataga ggtctttttc tttttctttt
    82741 atttttttaa tcttaaccct tgtctttatg tctttaaatt ttacaccttg cttactagac
    82801 caattttcta aatacttcat cttttttact tcattttcat aagcccacag accaaatttt
    82861 ttatccgtct cataaagacc ttttaaaatg ttttcatttt tctctaatat tgccttattt
    82921 ctgtaatcaa ataagttttc aagcaatagt ttatattggt cttttacatc atcaccttca
    82981 acatttgcag attccatgac tttttcttcc atatttttca tacttaaatc attgattaaa
    83041 aatacacctg ctgtagtagc taaagacaaa aatacaaatg ccattatacc tttgatgtac
    83101 ttattcttat ttttcagtat tttcaaagct acacctccca aatagtacac cttacaaatc
    83161 aaattacaaa gaatattctg tcacatatag taattttcat atttaataat tctctatttt
    83221 attatgtcca atattaaaaa ttaattgtat caatacactg ttttttttac aattttttat
    83281 tttataatct acttttttct tttttttata gtaatatata tgtatataaa tattttggta
    83341 tcattataat taatacatac taatggaggg ttttttatgc aaaaaaaagt cgcagcaatt
    83401 aatgacttat ctggaatagg aaaatgctca ttatcggttg ctatacctat actttcagca
    83461 ttaaaggttc aatgttgtcc atttccaacg gccatacttt caagtcaaac tggttatcct
    83521 gaatttactt ttttagactt taccgatgaa atggtaaaat attcaaatgt ttggaaaaat
    83581 ttaaaagtca attttgatag tatatatagt ggttttttgg gttcaaagca tcaaattgag
    83641 atagtggcta attttataaa cgattatccc aatgctttta tcgtagttga ccctgtgatg
    83701 ggtgataatg gagttatgta tccaatattc acagaagaaa tgagacatga aattaaagaa
    83761 ttagttaaac attcagattt aactactcct aatttaacag aagcttgttt cttaactgga
    83821 aatgattata ccaaaagcga ttataataga gatgagctta tatatattgc aaaatctgta
    83881 tctgatttgg gacctagcaa agtcgttatt actggtatat tagaagatga taatatatta
    83941 aatttggctt acgatagaga taatgaccat gtattcttta catcagtcaa atataataat
    84001 tgttcttata gtggaactgg agatatattc acatctatac tttgtggtat gttagtaaat
    84061 aaacatgatt tgggagtagc ggttaatact gcaacagatt ttatatataa aactataaat
    84121 tacacatctc aatttgatac tgatagaaat gatggagtta tgtttgaaaa ctttttatca
    84181 gatttaacta atatataaag tcaaaaaaac tatccatttt ataaatacct ataaatcata
    84241 aaatttaggt atttacaaaa tggatattat ttactaaact attggtattt caattgttac
    84301 catagcacca ccagttttca cagaattttc aatatataaa atcccattat gtttttgtac
    84361 aatattattt acaatataga gtcccattcc ataatgagat ttagaatttc tactcaaatc
    84421 tcctgtataa aactgttctg tagcttttga caaatctgct ttagaaaaac cacttccaca
    84481 atctgtaact acaaatttta agtatttatt acgtactgat acacttatgt atagtttact
    84541 ctggcttggt gaataatcta tagcatttga aattatattc ataatagctc tgaataacag
    84601 ttctctatct atagttatat ttttaggcaa attatcttgt ttataaaact ccacatctaa
    84661 gtttttagta ctagctaatg cttctatttg cactaagaca tcttttataa actcactcat
    84721 actgattgct tctttattta atatatagcc attctctgtt tttgatatat ctattaaagt
    84781 attaatatat ttttctattt gagtgacacc ctttgagata tattccatgt attctaataa
    84841 ctcaggattt tcatttattt caattaataa gtcagtattt ccatgtataa ttgttagtgg
    84901 tgtttttata tcatgtgcca gtgcagatat ctgttccttt ctattttctt ctagcatcca
    84961 ttgtttttct aaagaatctt ttaacgccaa cttcatttta tccatagaaa aaaggacatt
    85021 attaatttca taaattttac tagattctac aacaaaatct aaatcctgtt gttctatttt
    85081 ttctgtagta ttttttaaaa gctccatttc aatattaaat ttcttgccaa atacttttga
    85141 taatataaca atttcaataa aaaatattat actaaaaata atcataccca atgtctctgg
    85201 ttttggtaaa tatgctctta aaattggaga actatactct gatactattt tgtattttat
    85261 tataaaaact tcatctttct taaaaaactt tatataactt tctgaatatg catgcctttc
    85321 ttcaatatct ctcattaaat tccatacttc ttttgattct tttttattaa aggtccctga
    85381 aattaaactc ccatccaaag tataaacccc atactcatat atacttggaa ttatatcttc
    85441 agttactttt tcacttgaaa taatctgttc tttatattta ttaaattgtt tttctgcata
    85501 attagctggt aaaattacac ctgatgataa taatacagaa aatgataaca ttaaaaggag
    85561 tacaattgaa attgtcataa taaagaataa agataaatat tttataaaaa tagttcttaa
    85621 cttttcaact tttttgttgt tagatttttt acctccaagc atttgtaccc tgtcctccaa
    85681 tagtttaatt aattcataat taatatctaa ccagtttttt atttgattat tttatatctt
    85741 tttattgcta agttatattt taacataata atataaataa acagtcaatt tttactctat
    85801 tcttctgatt ttttaccttc ccatttagaa aaccaaatac ataaaattac aaatgctaaa
    85861 attgttacaa aaccacacat taaacctgtt gattgatatg cttttattga ttcaaccttt
    85921 atatattcaa ccgttttaga actcgaaaaa gacgaccaaa tagttataaa tctaacaccc
    85981 catgcacaag gaaaatactg ccaaatacca gaacccaatc cagttaataa taatgctgaa
    86041 agtaacattt caactatacc tactccaatt gatgttcctt ttccgaacct taaacttaag
    86101 aataaatgta atatgtattc aaataaatta cacccaatta aaattagaat tgaaatcata
    86161 taaaagcttg atgcaaagta agccttagat ataccaaatt taaaacctaa tacagacact
    86221 aatgttgcta caaaaccaag acatactaag attatatatt tacttataag agtaagatat
    86281 tttggattag atgttgttaa catatgttta taattccctg cataatattc ttgttcaaca
    86341 aacattgaac acacaacacc tgacaataaa ggaaaagcta ttgctataac ttgtaaatat
    86401 gctactatct ttagtatatt atcagctcct gaaaccttat aataccataa aaacaatccc
    86461 atccccatta aagcaatgca aatgtgcatt agtattataa aatttctctt taattttatt
    86521 aaatcagaac aaaatattct tattaactgt ctcatattac ttcacctctc tattattgaa
    86581 ccaaaccctt gtagctaaag caaataatac aaataaaaca atagatatta atactcctgg
    86641 caaaataaca gcattattta aaaattcact tccctctggt acaggaagac catttggcag
    86701 aatatgcaat acaacagcca ttaatcttga aggaatcgaa tatggaaaca tataccacat
    86761 ttctgtagtt gagaaaacag caactccaac aatagtaaag aacatattta ttagaactgt
    86821 aacaaatata ccaaattttg aagctaaaaa catacaaaga ggaatctgcc acataaatag
    86881 taaaataatt aatatagttg caatgatatt ttgacctatt gaaatagttt ctttatagat
    86941 tatcccaaca aaaactacac ctattgcaga aataattgat gctataaaac aacaaataat
    87001 acagtaaatc atttttccag tccaaacgtc tttcatttca attggcaatg gtaaaatacc
    87061 acgatattta cttttttcat cttttaatac tattaaagaa gatattaatg ttaatgctcc
    87121 tggaagaaat gtcgtatacc accagttgaa agcagcattt tgccctccac ctagtccaca
    87181 taaaagtata caaaacactg gaactatcca tatcaacttc attccaaaag ttcctttcat
    87241 cttttgaaat tccactctaa taaatctttc catattactg ccctctcttt ctattttcag
    87301 ctacaacttt cataaaaagt ttttctaagt tttgttcttt tgatacttct ccttgataac
    87361 ccattactcc attagaaatt attcctattt catctacaac ttgttcaact tcagacaaga
    87421 tatggcttga taaaataact gtaattcctt gttttggaaa agaacgaatc aattctctaa
    87481 gttcttcaat accaataggg tcaagaccat ttgtaggctc atctaagatt aaaagttttg
    87541 ggtgatttaa taatgcaata gcaataccaa ggcgttgctt catgcctaga gagaattgac
    87601 ctgaacgctt tttaaaggta tctcttaaat ctacagtctc taaaacttct tcaatgcgag
    87661 agtcaggaag accaagtaaa gtagtatgga cttttaaatt ttctcgtgct gttaaattct
    87721 catatatagc aggagactca ataagagaac caatatttga taaatccttt ctagtccatc
    87781 tatgtccctc aaacattatt tttcctgaag ttggatttaa aatcccagtt atcattttta
    87841 ataaagtaga tttcccagct ccatttggtc ctaataatcc atatatagaa ttagatttaa
    87901 ttgctagtga aatgttatca actgccatct gctccttaaa ttttttagaa actcctttta
    87961 cctgcaaaat ataattactc ataattttca tcctttcttt tgtcaaactt atttacaaga
    88021 atcattctaa atcataaata taaggatttt ataagaatta aaaaacaaaa aaagatgtgt
    88081 taaaaacaca tcttttttta tcttaattct attaattttc cattgtagta tatctctact
    88141 tcttcaatcc aaatgctatt atcttcttct tggatgacat ttttaataat attatttata
    88201 gtatatatta tattttgttc attcaaataa tcatctttcc aagaaactct acttttttta
    88261 tctggttcta tcaagtttat tttaacaaga ttatcttttt ccttaacttc tacttccatg
    88321 tctaaaccat taaaatactc atttgataat gtatctaaga ttaatcgcag tttatcctta
    88381 agtgaacttt cttcatcaat ctgtaagtac tttactattt tctcatcctt tgaaattatc
    88441 tctataggta ttttagtagt ttttttagaa ttaatcattt tactagttgt ttcttctttt
    88501 gcatcttcat ccttctttac aactgctgat tctttagaat caactagctc aactgcactg
    88561 atgctataat ccatacatgc cgtactcaat aaagtagtag caattattaa aaaacttact
    88621 gctattttat tcataagtat ccctctccta attctctttc gatacttatt ataccatagt
    88681 atagattata tttttatact tttaaatcaa tttataaatt attatagtaa attatttaca
    88741 attctaagct atttgaattt atttttatag tatattcttt ctctaaattc gacagatttt
    88801 tattcaaaag cccttttatt tctctcgtat ttctatactt gttgctatgt tcttcactat
    88861 gttcataatc atatgaatca ttgtcatatt cttcatcatt attaaattct tcaattgctt
    88921 cttttgactt tatatcacaa ttaaatttat acttatttac tggtatatca agtcttacct
    88981 tagaatttgg aacttctatg tccaggaact tagcaattgg catatcagag tacaagtaaa
    89041 attcactttc ataatttgaa tttctaccta ttttctcttt tatatttaca ttttcaggta
    89101 tatactcttc tatattattg ataaatacat cttcattctc tgaagatata tacaatgact
    89161 ctgattctat gtttacattt ttaacgccta aaattgattt tgttgacaag tgaagattgt
    89221 tattagatat taagtttagg ttttctaatc tatttacttt gttctctgtt atagctctac
    89281 taaaacctcc ataagaagtt ttatatgtta tagtatttga agctacatta tcgtatacaa
    89341 ataagtgtga gcttatactt gatgcattta tgtctacatt atttggtaca tatattataa
    89401 tttctctata atcatgtgaa aacatactat ttatagatgt atttaataat tggtcgaaat
    89461 ttttaatctt tttaatttta ttttcatatt ttctttcacc ttttacaact aaagtcttat
    89521 cttttaaagt tacattgtat ttgtaaaact cattatttcc tcttgttgtt acacgcacat
    89581 cttcacttga acttttcttt attgtaacgt ttatattgtc aacagctata tctaacttgt
    89641 caatatttac tttttcttga taaagtttat tttctttttt aaattcagct tccttttcta
    89701 aaccaaagtt tattaggctt ggcataacta aaatccctga ccatgttgtc cctattatac
    89761 ctattaccaa taaaaccgca gcaaatatag caagtttttt attcatgttt atcttctcct
    89821 ttcttatttt ctatatcttc aaggtcctta tatatcgcct caaaatttat actatcatca
    89881 ttatattcat cagtttcttt tatgctttct tcttctctat tgtagtaatc attattatat
    89941 gaatcttcat taatgtcttt attcaaatct tcatcattta aagtagcttt ttttcttttt
    90001 tccatctttt catttgcttt tatataaata ttttttctct ttatccaatg tatataatta
    90061 tttagtaatt gtttacctag ttttatggca aataataaaa tttgccatgc aagtatctca
    90121 agccctacaa atgctatggt tgcgaaaata caaaacatca taacatctgg aaccattgct
    90181 ataaatttaa tcacaaatgg aattaatata acaaaagcaa ttaatgagca tactaatgca
    90241 atagtagcag atacaatagc acatacaaac gcaaatgctg gaacaattag aaataaagat
    90301 aacactacta ataacccctt tataacttgt ctggataatc ctcctacacc ttcttctata
    90361 cttggtgtaa gtttatcatt tatattttct tttgatttat tgtacttttc cttagcttca
    90421 tctttcaaga tagaataatc cttttttaca tttgatttaa atctactaaa tttaccattt
    90481 aatatgtctg tattcattat ttttccatct atattttctt ttgatttact atacttttct
    90541 tttgcttcat cttttaagat agagtaacct tttttcatat ctgatttaaa tttactaaac
    90601 ttatcattca aaacatctgt atttattatt ttttcctctt caatattttt ttctttagtt
    90661 tgtaagataa gttcttttgc tatagatttt ggtgagccaa gtgatgctat tatctccata
    90721 tcactctttc cctctataag accatctaca aaatactctt cataatctct caatatatca
    90781 ttaacttcgc tctcagaaaa atcttttttt agataatcct ttaaaatttc aagaaactca
    90841 gttttattca agttcattac ctccctcatc ttcacttatt aaaatctcaa cagcctttac
    90901 aaattctctc cactcttcca tcagattgga taaattctct tttcctagtg aagttattct
    90961 atagtatttt ctagcaggac cttcattaga ttctagtata tatgtttcga aatacaaatc
    91021 ttttgtaagt cttcttagaa taggatatat tgttccttca tttacatcta ttactttaga
    91081 gatgttatgt actatctcat atccatacat atctttctta gatatgagag caagtacaca
    91141 aatttctaaa acaccctttc taaattgtgt gttcatatac tccatccttt ctttaaatac
    91201 acagtattgt gttttacaag gtactatgtt aaaacttctt atatcctcat aataacacag
    91261 tactatgttc tgcacaatag ttatttttaa atattcttgt tgattaaatc cttctataaa
    91321 tgtataatac tctaatcaga tatattttat gaaagtggtg ataaattggc taaaatgaaa
    91381 gatgataaaa caatattaat cctaactgct caatttggtg ctggacatat aagtgctgca
    91441 aaagcagtca aagaatgtat tattgaaaaa tatagtaact ataatgttgt tatacaaaac
    91501 tttataaatg caagtattcc catgatgaat aagcctatgg taaaacttta tgaaaacaat
    91561 acaaaatata ctcctggatt atataattac tattattact ttaagaaatc gtttgattca
    91621 agacatgatt tatctcataa attatataca cctaaacttt ctgagtatat tgctgatata
    91681 aatccagatt tgattatctc tacatttcca ctagctgctg cttgtgtaaa taactttaag
    91741 ataaaaaatc ctgatataaa tatacctaca cttacagtta taacagatgt tgtagatagt
    91801 atggaatggg tttttgaaaa tacagatttg tattttgttc cttctcctga aataaaaaat
    91861 agattttttc agaaaggaat aaatcctgac tccataaagg ttactggagt tcctgttgat
    91921 aagagatttc aaattgaaag taaagaacta tgttgtgata aatatagact attacttcta
    91981 ggtggaggaa gaggtctatt tgatatagat gaggatttta tgcattggat agatgaattt
    92041 attgaagaac atagtaattc tatagaaatt acaatagtta ctggtaaaaa caaaaaatta
    92101 tatgacaatc taactcataa aaaacctcta aaaaatatta aagtacttgg atttgtaaac
    92161 gacatgtata atctaataag agaatgtgac ctaatgctaa ctaagcctgg aggagcaact
    92221 atatttgaag ctattcaatc acaaactcca gtactcgtaa aaatgccaaa agttggacaa
    92281 gaaattgaaa acgctaaatt tataatagat aagggtcttg gcatgattta tagtgatgac
    92341 ctagacctta aaaacatatt ttataaatta gtatcaaatg aatttgattc tataataaac
    92401 tttatgaaaa aaaatttaaa agaatttaaa acagtcattc atccagagaa aattgctgat
    92461 tatatatcag aattaataga taatcattac agttaaattg tactattaaa aaatttcaaa
    92521 cataaaatta aaggcaatca agtatgattt tacacttgag aatttactca tagtgtttaa
    92581 taatacttga ttgcctttat catatcaaaa tcaaattgtt atattagtat atatttttct
    92641 tgtactgcat atacagttgt tgtatttctt ccatacttct ctttaattct atttgtaaat
    92701 ctgaagcagt ctggtaatcc ttattttcta tagcttctct cactctagtg aatagtgatt
    92761 ctctagttaa agtatccttc ataatattga actttaaatc cgatattgag ttccaaacta
    92821 cctcatcaag cgcatccata ttttcttttg tgtgtatctt gacaatactt cttatttttt
    92881 gaatcccagc ctctattatt ctagttttta atttcttttg ccacttatct atagctccaa
    92941 tcttaaatga ttctattata ctatcagtaa atacatcacc ttgttttaaa gattttaatt
    93001 ttgaagcata tatttctaag tttttcatat tttcataaac tgtagctggt ggtattccaa
    93061 atcttgcatt tctttcttcc aaactataat gttcaaatac atcattttca tctctatatg
    93121 ctctatcttt ttctaaatag aaactttctt cacctacatt tttagataac tctttttcaa
    93181 gttcttttgt actaagacca ctcttagctg ctgcttttat accatccaac attgtttgat
    93241 aacatcctgc tattactaaa tacgtgttag atagagggct tggtgacctt aattcaaatc
    93301 ttacagtctt tggatttttc atatctctta ttaatccaac aagcactgac ctatttcttg
    93361 atggaatctc ataactatgc cctaaagatg taactataca tacaggagct tcaaatccag
    93421 gaactaatct attaaatcca tcattagaag ctgttacaat tgggtttaat acttcataat
    93481 tgtaaagaag tcccataaga gccccatatc ctagttcact tagataatct tcttttaaat
    93541 cttttggagc aaatagattt tttatacttc catcctttaa cttcgcactt actcctacat
    93601 gtgtatgtcc accacttcct gctactccat gaattggttt tgctttaaat gtaacttcta
    93661 gtccatgtga tgtaaataca tcctctataa catctcttac taataattca ttgtctgctg
    93721 cttgtaatgg tgtagaaaac ttccaagaaa cctctaattg ctccatagca tggtttgttc
    93781 ttccatctat actaatagaa ctttgtattc ctccgacctc tttgtgagcc atttcagggt
    93841 ttacacctag cttttgaagt ataattaaac ttttctctaa acaagttctt ataataccat
    93901 gagttctttt ccagtattgc tcttttaggc tttgtgatac atataatttc tctaagtcag
    93961 ctttatcctc aggagtatta acccagaact caagttctgt agctgctgtt aacattattt
    94021 cctctatatc atctactgaa tctattccta tgttatttat tacatgtgga tattctctaa
    94081 atatttcata cattgacttt ttaaagtact tatctgcttt ttgaagtact cctctagaac
    94141 atactttttt attatcatgt attaaaaatg caggtatctt taatgttcca acaggtagac
    94201 ctacttcttc gtctatatat tccatattgt aatctacata ccaatgacaa cttttatctg
    94261 gcattaaatc tactttcgca ttatttaatg tagctatatt ataaagctca acacttgaac
    94321 catcagtctg tatagctgat tccaaaaatt tatctatatc ctccaaaaat aattctacag
    94381 gaatcttttc atctgttgca tttcctccta aatcaacccc cattaaggaa acaaatctta
    94441 tatttttatt gctttttagg atttctctta attcttcatt agtgtgttta tctttctcta
    94501 ttacgtataa tagactatcc atttttccac tctcccataa tttttatgta tattttattt
    94561 tgttaaatac aagataaatt tcattttcct tacatttatg aatttttctt gcaaattatt
    94621 cgtgttttat tcgtgttttt atttatatta ttatatatca agtataaaaa gttcgcaata
    94681 ttttttattg gaaaaaatat tatacataca atagtttaac atttttacaa atatggggta
    94741 tactaaaata aaagtatttt tatttgaaaa tttaatttta tttataatat aatctatagt
    94801 aaaagaaata ataaaaaaat ttaggagata aactttatgg aatacataat tttcgatttg
    94861 gaatttaacc aaggatttga taaaaaactc aataaaactg tatccaatga aaagtgtcct
    94921 tttgaaatta tacaaatagg agcaattaaa ttggattcta aattcaatat aatagatacg
    94981 tttaatagtt atgtaaaacc tacaatttat aaagacatac atccctttat tagtcgtatg
    95041 actaatatta aaaattctga ttttaatgat tcgcctactt tccctgaggt ctacaacaat
    95101 tttataaaat ttatatcatc acaagaccct atattatgtg tttggggagc tggagattta
    95161 aaggaattat acaggaatat caactatcac aagctaccat caaattcctt acctaaatct
    95221 tatattaata tacaacaaca tgcatctaaa tatttcaata atccagcagg aaaaagcatt
    95281 ggcctacaaa atgctatttc tatattagaa cttgatgaaa aaatgtctta tcacaatgct
    95341 ttaaatgatg catattatac tgctaaagtt tttattaaga tatacaatcc aagtatagtc
    95401 ccagatatat atctttatac aagtataaaa cctaaaacta taagatattc taacaaaaaa
    95461 agagttgatt atgataagtt atttgatgaa tttaggaaaa ttttaaatcg tgaattaact
    95521 aaagacgaga aaaaaatcat taacttagct tataatatgg ggaaaacaaa tcaatttaca
    95581 ttagaaaatg ttaagcaaag aaaaaacaaa tagagtttat ttagataaaa aataggcatg
    95641 tagattaaaa aactagttaa ctttaaattt atatctacag cccaattttt atcataatta
    95701 atttttattt tatctctaat tcaactggac aatggtctga acctaatatt tcagtgtgta
    95761 tatccgcact tactaatcta tcttctaatt ttttggatgc acaaaaataa tctattctcc
    95821 accctgcatt attttttctt gcattaaatc tatatgacca ccaactataa acaccttctt
    95881 tatcaggatt aaaatatcta tatgtgtcta taaacccact gtctaataat ttagaaaact
    95941 tctctctttc ttctttagta aaacctgcat ttttcatatt atttttagga ttttttaaat
    96001 ctatttcttt atgtgcaacg ttcatatctc cacacacaat tacaggctta tgattatcta
    96061 gtttggttaa ataatctata aagtcgtctt cccatctagt tctatattct agtctcttta
    96121 attcagattg agaatttggc gtatatactg taacaaagta aaaatcttca aactctagag
    96181 ttatcactct accctctttg tcatgttctt ctatatttat tccatacatt acttttaaag
    96241 gctctttttt tgtaaatata gcagtacctg aataaccctt tctctcagca taattccaat
    96301 attgaaaata tcctggtaaa tctaactcta tttgcccttc ttgcaattta gtttcttgta
    96361 aacaaaatat atctgcatct acttctttga aaaagtctaa aaatcctttc ccaacacaag
    96421 ctcttattcc attaacattc catgatataa atttcatact tatactcctc atctaaacat
    96481 tgacacactc cccatagcta aagatggggg cttttcaaca attttattgt aaattcttaa
    96541 ttcactttta ttttaacaat gctatactat aatttcatct aaaattacta tttacttaat
    96601 acctaaaaat atatgtttta atcaattttt aacttttttg ttaaatcttc agggctcatt
    96661 gaagaaacat acatatttgt tccaagttca aatcctccaa aattatattt tcttgggatt
    96721 atgtgcatat gaacattaaa atacgatttt gtttcaatct ttgttggatg agtgtgaata
    96781 cataaattaa atggcatata tccattgata ttatataact tcttaaacaa cttttcaaat
    96841 attgtagaaa gctcctgtat attattatca tttaactcac caaactctat tttatcttta
    96901 aatattattc ttacttctcc actatacttg gttgcatatg gtataagtac caaaaaagct
    96961 tctccattat aaacaactct tctatctaaa cttatttcat tttctattaa atcatcatac
    97021 aaagagcttt tattgctgtc ataatactcc tttgaaacca acagttcatt cgtaatttca
    97081 ggtggaataa aagacataga caatatttga gcatgtggat gcatcaaaga tgctcctgcc
    97141 tttctcaaga agtttttgaa gatactaaca tactctactt tatcatcttt cgacaaatct
    97201 ctaaatctat ttctatacat aaaaaaaaca tcttcaaatt ctttttgact catgttataa
    97261 aaactacgat tatgtttatc gctctctatc attacttcat gaacaccata tacatctctt
    97321 gccatatcat caattatagg aaatttattg tagacagatt ttacaagcca tccttcttca
    97381 ctttctattt caaataatgc atcagttgca tgagcttcat ttcctttgca aaatgggcat
    97441 tcttcatcat attcattagt ttcttcatct tcatttggga tatctgctgt atctaaaggt
    97501 ctttttaacc tatctgtagc aaatataaca acatcattgg taattggatc tattcttaat
    97561 tctttcataa ttaacacctc ttagttaata gcctttaata taattatatg atatttatcg
    97621 tatctgttaa ataaatttat aatatttttt ataattttgc atatttatta ttatatattt
    97681 tgtctatagc tgaaagatta taactaaaaa atcactatca gtatatcaga atattatctt
    97741 actataaatt aatttatgaa tacataattt agtggcataa acatgggtat tttactataa
    97801 tttgttaaaa gtttatttta aagctatatt aatttttttt gatgattttt acccataagt
    97861 atgttattat tatcttgtaa agatgtcttg tattcaaaca tacaatttac ataatacaat
    97921 atacgtattt aaattaaata gggggtatta aaatgaatta ttcaaacaga gttagtgcaa
    97981 tgcaagcctc tccaatcaga aaacttgttc catttgcaca agcagcaaaa gataaaggaa
    98041 tcaaagtata tcacttaaac ataggacaac cagacataaa aactcctaaa ggattttttg
    98101 atgctgttaa aaactttgat agcgaagtct tagaatatgc tacatcagaa ggtattccag
    98161 aattattaga agcacttcaa aattactata aaacttacga tatgaatttt gaaaaagatg
    98221 aattattagt aactaatggt ggtagtgaag ctttactttt cactatgatg gcagtttgtg
    98281 acccaggaga taatctatta gttccagaac cattctatac aaactacaat ggatttggtc
    98341 aatctgtaaa cgtagaagtt aatgctgtta caacaaaggc agaaaacggt ttccaccttc
    98401 catctaaaga agaaatattg tctaaagtag atgataaaac taaggctata attctttcta
    98461 acccaggaaa tccaactggt gcaatttata caaaagaaga acttaatatc ctagctgaaa
    98521 tagctaaaga aaaagattta tggataatag cagatgaagt ttatagagaa tttgtttatg
    98581 atgggcttga gtatacaagc tgtggtaact tagagggtgt acaggataga gttataataa
    98641 tagacagtgt ttctaagaga tacagtgctt gtggtgctag aattggttct atcgcttgca
    98701 aaaataaagg tcttatcgct caaatcctta aattatgtca aggaagactt tgtgttccta
    98761 cattagagca aataggagct gttgagttat acaaaactcc tgtttcttac ttcaaagaag
    98821 ttaatgaaga gtacaaaaaa agaagagatg tattatacaa tgaattaatg aaggttgaag
    98881 gtgttatatg taaaaaacca actggtgctt tctatatagt tgctaaatta ccagttgaaa
    98941 atgcagaaga tttcacaatt tggatgttaa aagaatttaa caaagataat gaaacagtta
    99001 tggtttgccc tgctgaagga ttctatgcta ctccaggttt aggacgtgat gaaataagat
    99061 tagcttacat cttaaatgaa aaagaccttc atagagctgc tacattatta aaagaaggtt
    99121 tagagcaata tgtagcttta caaaaataat tttttaaact taatttatat aaatttaatg
    99181 atataaaatg tccccttttc agatattatt tgaaaagggg acatttctta attaatatat
    99241 taattaagac ttagacctga ctatttttca ttttctttat atagtgttgg ccagtaatct
    99301 ttattcactt taattaaagc atctaaaact tgtcttgcct tagttggaga gattatagtt
    99361 ctatttaatg ttaaagataa aatagctttt tcataagatt gttcaaagta tgcatcaact
    99421 gctaactttt cataagctag ttgaccttct aacagtccct tataaaatgt tggtatatta
    99481 cctacatgta aaggtctcgg tccattcttt gtaagaatcg cagctacttc taccattgca
    99541 tcatcaggta agttgctaat agccccatta ttttctgtta taactatggc tatttggtgt
    99601 ttgttatgag ctattgcttc tgcaacttca actataaatt ccccatgagc atcattatgc
    99661 actaaattag aacctataga tgttcctttt tctataacag atttacattc ttcaaataca
    99721 cgcttttctc ttccattcat aacttctctt gctctagtac aatttatatt tgatttagct
    99781 acttttttat caggatatag ataatactgt acatatgtat taggtaagta atctggtaca
    99841 tcttccaaca tatctttaac cattccataa gtgtctaacc aagatttatc tctttgttct
    99901 gcatcaacag gttggaatcc agagtttgct actaaatctt ttatcttagg taccaaatct
    99961 tcaccatttt tatcatacaa atgtgtaaac catccaaagt gatttaatcc aaagtataca
   100021 ggctcaaatg ttcttgaatc catgtctaac aatcttccat aagaccttaa caagttaact
   100081 ggttggtcac acatattaag taatctttta tcatctggaa atactctttt taatgcttct
   100141 gcaacaatag cagcaggatt tgtataattt aatatccatg ctttaggaga gtattttctt
   100201 acagtcttaa ccatttctat catatcacct atagacctta acccataagc aaatccacca
   100261 gctccacaag tttcttgacc aaccagtcca aattgtagag gaatcttttc atctaattct
   100321 cgcatcttaa gaccaccagt tctcatttga acaaatacaa aatctacatc tataaatgct
   100381 tcatctgggt ttgtagtata ttcaagtgtt gctttaggat attcttcttt aaataatatt
   100441 tttccaaatt cccctattaa tctttgacgt tcttctacta catctaacat agttacttta
   100501 tttagaggaa atctatcact cattttacat aaagacttta aaagtcctgg agtccaagta
   100561 ctgcctcctc caactatagc tatattgtat ctttttttca ttgtcatatt aatcacccct
   100621 gttttttatt aactaacttg taagatgatt ataatatata aagttttcta catgaatact
   100681 tttaggtaat cattaaaaat agttcaattt tagtattttt ttcaaaattg taattttttt
   100741 catctattgt atttttcaat gtatttatga aacatatatt ccataagaga aagtgctggc
   100801 actctagatg tatagttaac actattaaaa ttatattgtg gagaagatac aaataatttt
   100861 atatcagcta tatttgataa tgaattgaca cttatatttg tcagagaaat tactttaact
   100921 tgatttgttt ttgctatatt acatgcctta ataacttgag cagtctctcc agacatagat
   100981 acagcaatta caaggtcatc tttagtaata tttgatgcag ccaaatacat gagagttgaa
   101041 tcatcatagt attttacatt tacacctata gctgaaaaac gttgagacat ttcagtacat
   101101 actaatctag aagacccaac accaaaaaaa tatatatgct tagaattatg tatttcattt
   101161 aaacacatat ctatattctc tacatttatt aaattttgtg tatccataac atcagtaaaa
   101221 atatcacttt gacttttagt aaactcctta tttctttcat ttctatttga atatgaaaaa
   101281 actaaaaagt ttttaaattc actatatcca gaaaagccga gcttctgaca aaaccttact
   101341 atgtatgcag tcgaattaaa agtttcattt gctatatctt taatcttttt ttgatttatt
   101401 ttttcagaat tttttagaaa atagtttaaa agagatattt catttgtaga taattttttt
   101461 tgatttatat ctaatttgtc tattaggttc atttatacct ccataataat gatttgtagt
   101521 taaacaactt tctcatattt attatataaa tatacactat acagcattta tcttatgttt
   101581 attatactat aaaaaagaca atacaaatta taaattagta ttgcctttta atatttactt
   101641 taaatagaca ttgcaaaaga aacttcgtct cttacttgag atacagatgg cccataaatt
   101701 atttggatat ctttatcaga tacttttata actcccattg cacctgtata ttttttaaaa
   101761 gcattttcat ctactttact catatcttta actactactc ttaagcgagt tatacagttt
   101821 gttatatctt ctatattgtc cttaccacct aaatatttaa ttatagataa tgctctctta
   101881 gtagtttctc cactttgagc tatttctact ttatttgata aaggttttaa atcaaaattt
   101941 atatctgtta tttcttctcg tcctggagtt tgtaagttaa atctaagtat caaaatctta
   102001 aatacattat aataaactat tgcatacact aaaccaagta ttatagaata aatccacttt
   102061 gtatttggtt gcataactcc aaatactact aaatctatta tgccaccttg agtatttcct
   102121 attgctactc caaatatatg catcatcata aatgatatac ctgataaaaa tgcatgtata
   102181 acaaataata ttggtgatac aaataagaat gaaaattcca atggttccgt aattcctgtt
   102241 acaaatgttg ctaaagcacc agctagtaat atacctttaa ccttcgacct attttcaggc
   102301 tttgcagttt tatacatggc atatgcagca gcaggaagac caaataccat tataggtatt
   102361 ttcccttgag ctaaaaatct agttgcttct gttgaaaaac tgattgtttc agggtcactt
   102421 agttgagcta agaatatgtt caatgctcca aatacttctt gccctcccac aactaaagaa
   102481 cctcctactt cagtaaatct aaacatagca tttaatatat gatgtaatcc aaatggtatt
   102541 aaaagtcttt caagaaatcc aaatacaaat gttcctaaat atcctaaact agctatacca
   102601 atacctacac tctgaataat tgtatttaca tatggccata tatatggtat tattatcccc
   102661 aaaatagaga atactactgt tgttattatt ggaacaaacc tagttccacc aaagaatcct
   102721 aaataatctg gcagttttat attatagtat ttattatgta atgatgctgt tattatacca
   102781 actacaattc caccaaatac tcctaattct aatgtttgta tgcctaaagt tacagcttga
   102841 cccatttctc tcatcatgtc aggttcagca ataatccccc tacaccctaa catataattt
   102901 acactagtgt gtaatactaa ataaccaaca aaaccagaaa atgcagaaac tgctttttct
   102961 tctttagcaa gaccaacaga tattgatata gcaaagaata ttgctaagtt tccaaaaagt
   103021 actcctgtta tagcttttgt aaaacccaat attccttgaa taaaattatt tgctaaaaaa
   103081 ggcatatcac taactatatt aggatttgaa aatgctgaac ttattcctaa aaatatacct
   103141 gctgcagata ttaatgctat tggcaataaa aatgattttc ctaactgtaa aaagaaccct
   103201 ttcatttttt gtttcatttc tatccctcca tttttaaatt tttaaaacgt tttctatgtc
   103261 ataattatat atttttattt tgtttttgaa actttataat ttgttttaaa atatttttac
   103321 ataaatagta attttttcaa aactgtaaat tttttcaaac aaagacatag taacgttgta
   103381 acatatataa atttaaaaag agcttagaaa ataaaattat tgaatataaa atggaaaaat
   103441 ataaattaca caaagagata tgagtacgta ctaacaaata cgaaaatgta taagaaatta
   103501 taaaaaagta catcaagaaa atttaagatg tactttttag attgtattta gacttataat
   103561 tatatagtta aatgaataac aaagaattaa tcttagtact attaaataca aaaattttta
   103621 tattttttct atctaaattc ttctttgttt aatatatatt gaagagtatt tattgcttct
   103681 aaatattttt ttctgtcttc ttcagttaat ttagaaagtt tttctcctaa accatcactt
   103741 aatttatcca taaaatcagt tgctatctta tctccctctt cagtaatgtc aattagatat
   103801 tttcttctat caactaaatc tcttttcctt gttacatact tcttatcagt taaatcatca
   103861 accatactag ttaaactgcc tttttctata tttaacttag agcataaatc agtcatactt
   103921 attactccat aattttttat gaaaactaat gctcttaatt gtgttttatt tacattataa
   103981 tctggtgcaa atttttttag gtcttctaag tacaggctag aataaatctt aggaaatgtt
   104041 tttactaaaa aacttatggt atcttttact atatagttca tataatcacc tcggttgttt
   104101 taaaatttat taaattatct aaataacttt atttcagttt acattaaaaa tattgcaagt
   104161 acaatagttt ttgctgaatg tttaaattta attagtaatc ttatcatttt tttattttta
   104221 tttatattat tcctattaaa tattatttat atagctattt tattaaattc atggtatagt
   104281 aagtaaagaa gaactcttca cagcggtgga gagaagacgc tagcccatta aaatggctat
   104341 cttatttata aataagatag ccgtttttaa ttatatttta tctgtacaat tctatactct
   104401 taagtctaca tctacttttc ctaagtcttc cttataatct tttatagcag gttctacaac
   104461 ttcatttaca aattctacaa cttgttctgg agctctacct ataaagtttt ttgggtccat
   104521 tatagatagt atttcttcct tgctcatttt aaatgaatca tcattcatta taagttcaat
   104581 taagttatta tccttgcctt catgctttac tctataggca gctttcatag aatattctct
   104641 tattatttca tgtaattcct gtctatctcc acctcttttt acagcttcca taagtattgt
   104701 ttctgtagcc ataaaaggaa gttcttcatt tacacgtcta tttatcatat tttcatatac
   104761 tactaaacca tcagttacat ttatccctat ttcaagtata gcatctgctg ccataaatgc
   104821 ttgaggtatt gccaatctct tattggctga gtcatctaat gttctctcta accattgagt
   104881 ggcttgtacc attgcagggc ttatagattc agagattata tacttagata aagatgctat
   104941 tctctcactt ctcattggat tcctcttata agccattgct gatgaaccta tttgattttt
   105001 ttcaaatggc tcttctaatt cttttaaact ttgtaaaagt cttatatcat tagtcatctt
   105061 atgcatactt tgagctatcc ctgatagtat gcttattact tgataatcta actttctagt
   105121 atatgtctgt ccacttactg cataggaaga atggtatccc atctttttac aaattttctt
   105181 atctaactct tttacttttt cgtggtctcc ttcaaataag cttaaaaaac ttgcttgagt
   105241 accagtagta cctttaactc ctcttaactt catgttgtct attctatagt ttaaatcttc
   105301 caaatcaaga attaaatctt gagcccataa acaagctctt tttcctactg ttgtaagttg
   105361 agcagcttgg aagtgtgtaa atccaagagc tggcatatct ttgtacttaa gtgcaaattc
   105421 ttttaaattg tttattaaat ttacaagctt tactcttaga agttttaaag cttcattcat
   105481 ttgtataaca tctgtattat ctccaacata tgcagatgtt gctcctaaat gtattattcc
   105541 tttggctttt tcacattgta aaccataagc ttttacgtga gacattacat catgtcttac
   105601 ttctttttct atttttctag cttcatcaaa atttatatta tctatattag ctcttagctc
   105661 atctaattgt tcttcagtta tattgattcc taactcatgt tcaccttctg caagtgcaac
   105721 ccataacctt ctccatgtag agaatttaaa ttgtggagaa aatatataac tcatgtcctt
   105781 gctacaatat ctgtctgtaa gtgggtttga gtaagtagta tatttattat ctatcattat
   105841 attagcacct tccttaaatc ttttgtcgtg tgattactgt gtacacatta gtacataaat
   105901 aattatatat aattttacga actataacaa gactttttta ttttttgttc acatttatat
   105961 atagtatttt ttatttatta ctatttttta tttatatcca aattatcttt ttcaattaag
   106021 taatccacta tttcactatc aacaaaattt aatacatact tatatctaat tgcatagtat
   106081 ttatcactcc atccaagctg attttttaca tcactttcat ctgccccaag ccttaaactt
   106141 attgctgcaa atgaatgtct aaaatctctt gctgaatatt gagaaagtcc agctaaatct
   106201 aatgcttttt tgactataag tctgacattt ctatctgtta tagaattact cttttgagtt
   106261 gtaaaaataa aatcatcact aggtattatt acctctggaa gcccagaata attcctgtaa
   106321 tcttctaaaa gtttaaaaaa gtatggatgt aatttaacta ctctttcttt tcttccctta
   106381 cctaacctta catatgagtt atcgttttca tctaccataa ggtctttcca ctttaagcta
   106441 actaattcat ttaataaaca cccagtagta actagacaga ctattattac tctatctctt
   106501 atatttaatt ttggtaggat acctataagt ttatttattt cttgtatact tagaacatca
   106561 tcttttgttt ttattcttgt tactgtgggt ttttctacat atctaaatgg atttatatct
   106621 atgtatcctt ctttttttaa gaagttataa aaactatgta aataactata tatcttttca
   106681 caagttgatt tcgcatattt agtctgtata tagtctacaa aatttttaca atcttctttc
   106741 gtagcataaa tcaattcttt cccttttaaa aattctttaa acagtataac ctttgataaa
   106801 taatcgtgtt tcgtgtttgg atttaatttt gtagaaaaaa cattaaaagt ttcatactca
   106861 taatcatcta gtatttttgt actatacata ataactcctt tccttttcat aaaataaaat
   106921 agctatactt ttactatacc atatatttcc ccctcaaata taccccttat tcaatagata
   106981 acttttaagt ttattttgtt atattgtgaa ggttttatga aaatatcaat cttccattgt
   107041 gcactacaca gtactatggt attataagtt agtaaaaggt ggcatttttt tattaatcta
   107101 tgaataaaat atgctactat aaatttgtat tggaggtatt ttgatttgga aacatttagt
   107161 ttgcttgaaa tttttaaggc agtcattttg ggcatagttc agggaataac agaatggcta
   107221 ccagtaagta gtacagggca tatgatatta gttgatgagt ttataaaatt gaatttttca
   107281 aatacattta taagtacatt tttagttgta attcaatttg gttcaatatt agctgtactt
   107341 gttatatttt ttagaaaatt aaatcctttt gatagcgcaa agaatattaa gcagaaaaaa
   107401 gaaacagtcc gtttatggct taaggttata attgcagtta taccatctgg agttattgga
   107461 atactgtttg aagatgatat agatagatta ttctttaatt caacagtagt tgctattgct
   107521 cttattgtat atggtataat aatgataggg cttgaaaaga gaaataaaaa acctaagtat
   107581 aaagattttt ctcaagttac atataaatta gctctttgta taggattatt ccagtgtcta
   107641 gctcttatac caggtacatc taggtctggt tctactataa taggtgcagt attacttggt
   107701 acttctagat atgtagcagc agagttttca ttcttcttag ccatccctac aatgcttgga
   107761 gctagtgcat taaaacttct aaaagctgga tttggattta ctggatttga atggcttatt
   107821 ttaggtgttg gttctgtagt agcatttgtt gtttcaattg tagttattaa attttttatg
   107881 gactatatca aaaaacatga cttcaaagta tttggttatt atagaatagt cttgggtata
   107941 gtggttcttg cttatttctt tctattataa tgtattctaa tttaataaca aatgataagg
   108001 agaaaagtta tgagtgttaa aaaagaaata tttgattgga ttaagtcaat agctatggct
   108061 attgtacttg catttgtaat tctacaattt ataagacctt ctattgtaag tggagaatca
   108121 atgtatccta ctttagatga taaagattat ctgattttaa ataggatatc atacaaggtt
   108181 ggtaaacctg aaaaaggcga tattgtagtt tttaaaacca atttagttga tggagaaaca
   108241 ggaaagaaaa aagacttaat aaaaagagtt atagctactg aaggtgacag aataaaaata
   108301 tcaaattcta aagtgtatgt aaatggaaaa ttattaaatg aaccatatat acacaataac
   108361 tatacttctg gagatataga tactgttgtt ccaaaaggta aactatttgc aatgggagat
   108421 aatagagaaa atagtaatga tagtagattc cctgatgtag gtatggttga tgaagatgaa
   108481 gttcttggta aggttatggt gagactatta cctcttgata atattgggaa agtagactaa
   108541 aaaataagag actgaattgg taaagtttat tttacaaatc tcagtctctt tttattttct
   108601 taaattacaa atattttttt actaaatatc ttttgattct gtaataagtc ttattatatc
   108661 acttgcttct tcattaaaag ttgttactat acttaggtag tctgttgctc tactcatagc
   108721 taaataaacc ttatttaaat ctcctacaaa gtcatttact tgataatctt gttcaatatc
   108781 atttatagtt tgattgtata acatctctaa ttcacatact attaccgctt tatactctaa
   108841 attctttatt gcatatatat ttgctattgt agttcctacc ttcttactta tatttgtcaa
   108901 attatcttct gcacatatat aaggaatttt tgcctcttcc aatgcttttt ttaacatata
   108961 ttgaaagtaa atggtttttc cactttttaa tttcttctta ttgtaaggat aaacgatagc
   109021 aacttcagaa taatcaagac cttttttact tattaaatac tcaatttccc agattacaga
   109081 acttatttgg tcatcaaggt cacttacttt tattatatca acagacttat ttccacctct
   109141 aagtgcttta gtataaaaga aagtactaaa ttctgtattt ggtcttaatt cgttgatata
   109201 tgagttagaa ttatttgaaa atttatttgt aaaatctaca atctctttag cttgtctata
   109261 atttttactt aagataatct tatcatcaaa ttctatacca ttatataatt ttttaaatat
   109321 attcaatcta ttagagatat tcaaggagtt acaagaacac acattaaata tgaattttgt
   109381 tttatataaa aactccctta taaaatctat ttcatactct aaaaagcttt ctgcttcatc
   109441 aataaaaata gccttgaaca tatttttatt ttttataatg ttctgtgctt gttttacaag
   109501 attattaaag gtcttttcgt aatcattttt taacatgtta taatctacaa ttaaattgaa
   109561 ttttttagct agtttaaata taaaagaact aaatgtgtga acttctaaat tgttattatc
   109621 tttgtataat aactctatcc tctctcgaag ctcattgcac aattgttttg tatgtgtaaa
   109681 tatgactaat ttatgatgtg gatatacttt tgctaatttt atagccctag aaagcataat
   109741 tgctgttttg cctataccag aaccaccttc aataagtgta ttcccatatt taatagaaac
   109801 aacatcttta atttgaacgt cttccatcat agttgctgta tacttatagt catcattgta
   109861 gaatgaaatt tttttaaatt tttcatttag atgaagttta tcatttatta tatagtattc
   109921 tgaacatatg tctagtaaaa ataaatttaa gtttatttca tcattctcat cattaagata
   109981 ttcctccagc gaacccttat tcattatatc ttgaagctta tttttatcta ttatattatt
   110041 attaacaaat tcttcaaatt catatgtttc ctcaacttca acgtatggca taatgtaaac
   110101 gtagttataa cttatatttc tattcttttg tcccattttt agtttcaaaa gttcatattc
   110161 ctcattcata acttcaagta actcttcttc taaaataaaa aatatatctt ctgttgtatc
   110221 cataaatttt ataaataaaa ttcttccatc tttaacatac aacaaatcgg tatttattcc
   110281 tttaaaagga gtaacttttg gcactacttt aacgtcctca ttgatatttt cgtaaaatat
   110341 tttctcgaac tccttcaatt ttagggcgtc taactgagaa tttatattta tgtattggct
   110401 cacttacatt acctccttct gttatactta attataccat tttgaactat atttttatat
   110461 atacttttta taggcaaatt tagtaaataa attcaaaata aatacaaata aatacaaaac
   110521 tgctaaaaaa attttgcaat aagtat
//
LOCUS       DAMJWS010000002       106882 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMJWS010000002 DAMJWS010000000
VERSION     DAMJWS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 106882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.002"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            109..300
                     /locus_tag="PC399_000101"
     CDS             109..300
                     /locus_tag="PC399_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF2590980.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="PC399_000102"
     CDS             455..1864
                     /locus_tag="PC399_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2590981.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1995..2684
                     /locus_tag="PC399_000103"
     CDS             1995..2684
                     /locus_tag="PC399_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF2590982.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="PC399_000104"
     CDS             2687..3622
                     /locus_tag="PC399_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF2590983.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMNGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="PC399_000105"
     CDS             3619..4512
                     /locus_tag="PC399_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF2590984.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="PC399_000106"
     CDS             4590..5336
                     /locus_tag="PC399_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF2590985.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="PC399_000107"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="PC399_000107"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF2590986.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFIFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="PC399_000108"
     CDS             6193..6897
                     /locus_tag="PC399_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF2590987.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="PC399_000109"
     CDS             complement(7085..7513)
                     /locus_tag="PC399_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2590988.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="PC399_000110"
     CDS             complement(7550..8980)
                     /locus_tag="PC399_000110"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF2590989.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="PC399_000111"
     CDS             9112..9696
                     /locus_tag="PC399_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF2590990.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="PC399_000112"
     CDS             9712..10233
                     /locus_tag="PC399_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HDF2590991.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="PC399_000113"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="PC399_000113"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF2590992.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="PC399_000114"
     CDS             12596..13366
                     /locus_tag="PC399_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF2590993.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13703..14134
                     /gene="phnG"
                     /locus_tag="PC399_000115"
     CDS             13703..14134
                     /gene="phnG"
                     /locus_tag="PC399_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF2590994.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14149..14724
                     /gene="phnH"
                     /locus_tag="PC399_000116"
     CDS             14149..14724
                     /gene="phnH"
                     /locus_tag="PC399_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF2590995.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14727..15812
                     /locus_tag="PC399_000117"
     CDS             14727..15812
                     /locus_tag="PC399_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF2590996.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15832..16701
                     /locus_tag="PC399_000118"
     CDS             15832..16701
                     /locus_tag="PC399_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF2590997.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16694..17560
                     /locus_tag="PC399_000119"
     CDS             16694..17560
                     /locus_tag="PC399_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2590998.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17601..18314
                     /locus_tag="PC399_000120"
     CDS             17601..18314
                     /locus_tag="PC399_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2590999.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18315..19484
                     /locus_tag="PC399_000121"
     CDS             18315..19484
                     /locus_tag="PC399_000121"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF2591000.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19488..20324
                     /locus_tag="PC399_000122"
     CDS             19488..20324
                     /locus_tag="PC399_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2591001.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20325..21320
                     /locus_tag="PC399_000123"
     CDS             20325..21320
                     /locus_tag="PC399_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2591002.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21338..23026
                     /locus_tag="PC399_000124"
     CDS             21338..23026
                     /locus_tag="PC399_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2591003.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGTSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23038..24105
                     /locus_tag="PC399_000125"
     CDS             23038..24105
                     /locus_tag="PC399_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2591004.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDVMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24542..25537
                     /locus_tag="PC399_000126"
     CDS             24542..25537
                     /locus_tag="PC399_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2591005.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            25555..27243
                     /locus_tag="PC399_000127"
     CDS             25555..27243
                     /locus_tag="PC399_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2591006.1"
                     /translation="MITIKNKRRISDSKIQKCFLVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMAITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            27255..28322
                     /locus_tag="PC399_000128"
     CDS             27255..28322
                     /locus_tag="PC399_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2591007.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            28514..29044
                     /gene="rnmV"
                     /locus_tag="PC399_000129"
     CDS             28514..29044
                     /gene="rnmV"
                     /locus_tag="PC399_000129"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF2591008.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            29215..30084
                     /gene="rsmA"
                     /locus_tag="PC399_000130"
     CDS             29215..30084
                     /gene="rsmA"
                     /locus_tag="PC399_000130"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF2591009.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            30150..31415
                     /locus_tag="PC399_000131"
     CDS             30150..31415
                     /locus_tag="PC399_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591010.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEGSEE
                     EYIIEEYDEDEYEDYWEDDEDIDEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQ
                     GESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYL
                     GYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGF
                     LRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            31566..32681
                     /locus_tag="PC399_000132"
     CDS             31566..32681
                     /locus_tag="PC399_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2591011.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            32867..33763
                     /locus_tag="PC399_000133"
     CDS             32867..33763
                     /locus_tag="PC399_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2591012.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            33833..34630
                     /locus_tag="PC399_000134"
     CDS             33833..34630
                     /locus_tag="PC399_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591013.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(34685..36037)
                     /locus_tag="PC399_000135"
     CDS             complement(34685..36037)
                     /locus_tag="PC399_000135"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF2591014.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            36256..37134
                     /gene="purR"
                     /locus_tag="PC399_000136"
     CDS             36256..37134
                     /gene="purR"
                     /locus_tag="PC399_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF2591015.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            37290..37568
                     /gene="spoVG"
                     /locus_tag="PC399_000137"
     CDS             37290..37568
                     /gene="spoVG"
                     /locus_tag="PC399_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF2591016.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            37781..39160
                     /gene="glmU"
                     /locus_tag="PC399_000138"
     CDS             37781..39160
                     /gene="glmU"
                     /locus_tag="PC399_000138"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF2591017.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            39255..40205
                     /locus_tag="PC399_000139"
     CDS             39255..40205
                     /locus_tag="PC399_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF2591018.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      41049..41134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            41296..41817
                     /locus_tag="PC399_000140"
     CDS             41296..41817
                     /locus_tag="PC399_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2591019.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTTTDKAGLTILETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTDTEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDELTDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            41969..43645
                     /locus_tag="PC399_000141"
     CDS             41969..43645
                     /locus_tag="PC399_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2591020.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            43657..44865
                     /locus_tag="PC399_000142"
     CDS             43657..44865
                     /locus_tag="PC399_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2591021.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            44880..46592
                     /locus_tag="PC399_000143"
     CDS             44880..46592
                     /locus_tag="PC399_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF2591022.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            46585..47493
                     /gene="pilO"
                     /locus_tag="PC399_000144"
     CDS             46585..47493
                     /gene="pilO"
                     /locus_tag="PC399_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF2591023.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            47520..48086
                     /locus_tag="PC399_000145"
     CDS             47520..48086
                     /locus_tag="PC399_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2591024.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            48123..48650
                     /locus_tag="PC399_000146"
     CDS             48123..48650
                     /locus_tag="PC399_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2591025.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            48671..50209
                     /locus_tag="PC399_000147"
     CDS             48671..50209
                     /locus_tag="PC399_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591026.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRNSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            50309..51391
                     /locus_tag="PC399_000148"
     CDS             50309..51391
                     /locus_tag="PC399_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF2591027.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            51372..52154
                     /locus_tag="PC399_000149"
     CDS             51372..52154
                     /locus_tag="PC399_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2591028.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILSGKQLLELYFSMVI"
     gene            52256..52906
                     /locus_tag="PC399_000150"
     CDS             52256..52906
                     /locus_tag="PC399_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2591029.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            52920..53480
                     /locus_tag="PC399_000151"
     CDS             52920..53480
                     /locus_tag="PC399_000151"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF2591030.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            53497..56883
                     /gene="mfd"
                     /locus_tag="PC399_000152"
     CDS             53497..56883
                     /gene="mfd"
                     /locus_tag="PC399_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF2591031.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            56914..57909
                     /locus_tag="PC399_000153"
     CDS             56914..57909
                     /locus_tag="PC399_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2591032.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            58033..58572
                     /gene="spoVT"
                     /locus_tag="PC399_000154"
     CDS             58033..58572
                     /gene="spoVT"
                     /locus_tag="PC399_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF2591033.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            58692..60305
                     /locus_tag="PC399_000155"
     CDS             58692..60305
                     /locus_tag="PC399_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2591034.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            60412..61860
                     /gene="mazG"
                     /locus_tag="PC399_000156"
     CDS             60412..61860
                     /gene="mazG"
                     /locus_tag="PC399_000156"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF2591035.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            61949..62227
                     /locus_tag="PC399_000157"
     CDS             61949..62227
                     /locus_tag="PC399_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2591036.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            62299..62541
                     /locus_tag="PC399_000158"
     CDS             62299..62541
                     /locus_tag="PC399_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF2591037.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYKILE"
     gene            62603..62857
                     /locus_tag="PC399_000159"
     CDS             62603..62857
                     /locus_tag="PC399_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF2591038.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            62858..63391
                     /locus_tag="PC399_000160"
     CDS             62858..63391
                     /locus_tag="PC399_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591039.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            63397..63681
                     /locus_tag="PC399_000161"
     CDS             63397..63681
                     /locus_tag="PC399_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF2591040.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            63794..64714
                     /locus_tag="PC399_000162"
     CDS             63794..64714
                     /locus_tag="PC399_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF2591041.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            64804..67176
                     /gene="spoIIE"
                     /locus_tag="PC399_000163"
     CDS             64804..67176
                     /gene="spoIIE"
                     /locus_tag="PC399_000163"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF2591042.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            67527..69419
                     /gene="pepF"
                     /locus_tag="PC399_000164"
     CDS             67527..69419
                     /gene="pepF"
                     /locus_tag="PC399_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2591043.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLEADNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            69533..70498
                     /gene="galU"
                     /locus_tag="PC399_000165"
     CDS             69533..70498
                     /gene="galU"
                     /locus_tag="PC399_000165"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2591044.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            70998..72602
                     /gene="rho"
                     /locus_tag="PC399_000166"
     CDS             70998..72602
                     /gene="rho"
                     /locus_tag="PC399_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF2591045.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            72755..72955
                     /gene="rpmE"
                     /locus_tag="PC399_000167"
     CDS             72755..72955
                     /gene="rpmE"
                     /locus_tag="PC399_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF2591046.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            73100..73993
                     /locus_tag="PC399_000168"
     CDS             73100..73993
                     /locus_tag="PC399_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF2591047.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            74011..74859
                     /gene="prmC"
                     /locus_tag="PC399_000169"
     CDS             74011..74859
                     /gene="prmC"
                     /locus_tag="PC399_000169"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF2591048.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            74903..75967
                     /gene="prfA"
                     /locus_tag="PC399_000170"
     CDS             74903..75967
                     /gene="prfA"
                     /locus_tag="PC399_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF2591049.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            76096..76776
                     /locus_tag="PC399_000171"
     CDS             76096..76776
                     /locus_tag="PC399_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF2591050.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            76805..77845
                     /locus_tag="PC399_000172"
     CDS             76805..77845
                     /locus_tag="PC399_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF2591051.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            77862..78311
                     /locus_tag="PC399_000173"
     CDS             77862..78311
                     /locus_tag="PC399_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF2591052.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            78328..78780
                     /gene="rpiB"
                     /locus_tag="PC399_000174"
     CDS             78328..78780
                     /gene="rpiB"
                     /locus_tag="PC399_000174"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2591053.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            78816..79445
                     /gene="upp"
                     /locus_tag="PC399_000175"
     CDS             78816..79445
                     /gene="upp"
                     /locus_tag="PC399_000175"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF2591054.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            79746..80561
                     /locus_tag="PC399_000176"
     CDS             79746..80561
                     /locus_tag="PC399_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF2591055.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            80787..81224
                     /locus_tag="PC399_000177"
     CDS             80787..81224
                     /locus_tag="PC399_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2591056.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            81409..81636
                     /locus_tag="PC399_000178"
     CDS             81409..81636
                     /locus_tag="PC399_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF2591057.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            81641..82027
                     /locus_tag="PC399_000179"
     CDS             81641..82027
                     /locus_tag="PC399_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF2591058.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            82030..82734
                     /gene="atpB"
                     /locus_tag="PC399_000180"
     CDS             82030..82734
                     /gene="atpB"
                     /locus_tag="PC399_000180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF2591059.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            82803..83063
                     /gene="atpE"
                     /locus_tag="PC399_000181"
     CDS             82803..83063
                     /gene="atpE"
                     /locus_tag="PC399_000181"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF2591060.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            83186..83683
                     /gene="atpF"
                     /locus_tag="PC399_000182"
     CDS             83186..83683
                     /gene="atpF"
                     /locus_tag="PC399_000182"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF2591061.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLVEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            83680..84225
                     /locus_tag="PC399_000183"
     CDS             83680..84225
                     /locus_tag="PC399_000183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF2591062.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            84242..85744
                     /locus_tag="PC399_000184"
     CDS             84242..85744
                     /locus_tag="PC399_000184"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF2591063.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            85772..86650
                     /gene="atpG"
                     /locus_tag="PC399_000185"
     CDS             85772..86650
                     /gene="atpG"
                     /locus_tag="PC399_000185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF2591064.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            86663..88057
                     /gene="atpD"
                     /locus_tag="PC399_000186"
     CDS             86663..88057
                     /gene="atpD"
                     /locus_tag="PC399_000186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF2591065.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            88060..88320
                     /gene="atpC"
                     /locus_tag="PC399_000187"
     CDS             88060..88320
                     /gene="atpC"
                     /locus_tag="PC399_000187"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF2591066.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            88782..89162
                     /locus_tag="PC399_000188"
     CDS             88782..89162
                     /locus_tag="PC399_000188"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF2591067.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRDEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            89177..89638
                     /locus_tag="PC399_000189"
     CDS             89177..89638
                     /locus_tag="PC399_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2591068.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            89659..90249
                     /locus_tag="PC399_000190"
     CDS             89659..90249
                     /locus_tag="PC399_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591069.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            90264..91421
                     /gene="alr"
                     /locus_tag="PC399_000191"
     CDS             90264..91421
                     /gene="alr"
                     /locus_tag="PC399_000191"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2591070.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            91454..91741
                     /locus_tag="PC399_000192"
     CDS             91454..91741
                     /locus_tag="PC399_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF2591071.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            91710..92093
                     /locus_tag="PC399_000193"
     CDS             91710..92093
                     /locus_tag="PC399_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF2591072.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            92264..93091
                     /locus_tag="PC399_000194"
     CDS             92264..93091
                     /locus_tag="PC399_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2591073.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            93084..94025
                     /locus_tag="PC399_000195"
     CDS             93084..94025
                     /locus_tag="PC399_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2591074.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            94448..95542
                     /locus_tag="PC399_000196"
     CDS             94448..95542
                     /locus_tag="PC399_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591075.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            95768..96184
                     /locus_tag="PC399_000197"
     CDS             95768..96184
                     /locus_tag="PC399_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591076.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(96366..96935)
                     /locus_tag="PC399_000198"
     CDS             complement(96366..96935)
                     /locus_tag="PC399_000198"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF2591077.1"
                     /translation="MKKEFRKKVIESRKNKNANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            97089..98246
                     /locus_tag="PC399_000199"
     CDS             97089..98246
                     /locus_tag="PC399_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF2591078.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            98850..99332
                     /locus_tag="PC399_000200"
     CDS             98850..99332
                     /locus_tag="PC399_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2591079.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF
                     "
     gene            complement(100031..101185)
                     /gene="nagA"
                     /locus_tag="PC399_000201"
     CDS             complement(100031..101185)
                     /gene="nagA"
                     /locus_tag="PC399_000201"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2591080.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYIPALSCNIDNVCGSIHQGMEADFIVTDKDFNIYETYISGICKYKVNK"
     gene            complement(101268..101993)
                     /locus_tag="PC399_000202"
     CDS             complement(101268..101993)
                     /locus_tag="PC399_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2591081.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            102260..103189
                     /gene="pfkB"
                     /locus_tag="PC399_000203"
     CDS             102260..103189
                     /gene="pfkB"
                     /locus_tag="PC399_000203"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2591082.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVSK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYYVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(103318..104235)
                     /locus_tag="PC399_000204"
     CDS             complement(103318..104235)
                     /locus_tag="PC399_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2591083.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMQFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAISGEECIKAQRLLEKIINSVK"
     gene            104705..105874
                     /locus_tag="PC399_000205"
     CDS             104705..105874
                     /locus_tag="PC399_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2591084.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIVEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            105923..106774
                     /gene="gatY"
                     /locus_tag="PC399_000206"
     CDS             105923..106774
                     /gene="gatY"
                     /locus_tag="PC399_000206"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2591085.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
BASE COUNT        42618 a        11675 c        19282 g        33307 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atatcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagtgagagg cttcctaaaa cagctcttga
     1081 gcaaggtgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttataacttt
     1321 caaaaataaa gttgattcaa atattaatat atacgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaataa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagtatagg
     1681 taaagtttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag atatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttggtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagttaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aataatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgaatgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata catttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggcattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc tgcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag gccagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gcgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaat attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttattt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaaa acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agctgggaac atttttacat gatataggta agcctgacag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatgc tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac cttcgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagttatgc tgatatctga aaagccagca ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacggcagc cctagcccca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agatgcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa gaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaaag gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt tactataatc aaactaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag tagtttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atgggttaaa agcagcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaaaggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactaa ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacta
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcgaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgtgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggga atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggtacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttctca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatcttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagtttat gaggcactct ttttgtatga aatcaaatat attttcatca tttcaacaat
    13501 gaaaaagaag tttatcatat tttgagatag tttgttttta tttatttaat tcatatttta
    13561 aaatatattt tcttaacatt gttaaaaaag aatttacaag atattcatta gaattaactc
    13621 aactctaata ttcacttaac ttaagttgtt tattatatta ttaaagagaa gtaaacgaaa
    13681 agtagattaa ggaggttgaa ttttgtatac agttgatagt actaagatat tgataaatat
    13741 gaacttagag aaactagaaa agatatactc aatgataaaa gatacatcaa gtatttctat
    13801 aattaaagaa cctaacttgg ctacagtaat ggtaagagct aatgaaagtg taaaaaacac
    13861 aacatttaat ttaggagaga tattagttac tgaatgtagt gtaaaggtag atgaaagctt
    13921 gggttacgga atagtttcag aaaacaataa taagaaagcc atttatttag cagttataga
    13981 tgcagtttta catagcaata acagtaaatt tgatgagtta aagaattaca taaataaaag
    14041 tgtatatgaa gagaacttaa ggtatgaaca agagattatt gatgaattta gcttgattaa
    14101 taaaacgaaa gttcaattca atgctatgga ttaaatagga ggagaatcat ggtaaattat
    14161 acacaagaaa tatatagaaa gatgctagat agtgcttcat ttcctgggaa attaaataat
    14221 atagataata taaatataga aaataataca aaactattga atggagcaat tggtatagca
    14281 atcactttat tagaccaaga agttaccttc tatatagaaa attatactca aaaagatata
    14341 aaaaatataa gagcacttac aatgtcaaat caaatagatt atagaaaatc agattatata
    14401 tttttagata ttaattcaga agtggatata ttacagatta aaataggttc ttttgaatat
    14461 cctgatgaaa gtgcaactat agtacatcaa gtaaatgatt tatcaacgta ctctcaaact
    14521 aagtatataa agcttgattt gagtggtcct ggtataaaat ctaaaaatag tatatatata
    14581 aatggaatta gagaagaatt tatagaaaga ttatctagca taaataaaga ttatccaata
    14641 ggaatagatt tgattttagt agataaaaaa ggagaaattg catttatacc aaggtcatca
    14701 aaattaagtt gggaggtatt atagaaatgg cttatgtagc agttaaaggt ggagaagaag
    14761 ccatattaca ggctaaaaat atacttgagt tttatagggt aaaaggagat agcttgccta
    14821 tagatgtaaa gcagattgag gaacaaatgt cttatgctgt agacaaagta atgtctgaag
    14881 gaagtttata caacaaggaa ctatcagcat tggctataaa acaagctcaa ggtgatatat
    14941 tagaagcctc tttctatgta agagctttaa ggtcaacaat gcctagaatt acatattcag
    15001 aacccataaa tacagaagaa atgagaataa aaagaagaat atcagctact tttaaagata
    15061 taccaggagg tcagtatctt ggggcaacta gagactatga aaatagaata ttgaatgtag
    15121 aattattaga agaagatgaa aaaaagaata gacattttag agatagtttt ataaatgaat
    15181 tagaaacagt agatgaaatc cctgaattta caaaaatatc taatatattt agaaaagaaa
    15241 atttattaca agaagaagtt tgtgaaaaca aattggaaga agttattgat gatattacaa
    15301 gaaatagttt aagatttcca gtttctaggt cagcaaagtt acaggcatta actagaggtg
    15361 aaagtggagt agtgtcaggt tttgcttatg ctattgtaag aggttttggt gatgaacatc
    15421 catatataag tgaacttaga actggtaatg tatttataaa agtaaaacat cccatctatg
    15481 aagatgaaga aataataata ggggatatgc tggttactga atgtgaaata gtttcaaaag
    15541 catcacaaaa taagactgaa aattctaaaa ctaattcgga agataaaaat aagatgtctt
    15601 taggatatgg tctttgtatg gggaataatg agaataaggc tatatctatg gccatactag
    15661 acaaatcttt ggagccaaat agtgaaaaac tagcacaaga tgaagaattt gtattgcttc
    15721 atatagatgg aatagattca ttaggattta tatctcactt taagcttcct cactatgtag
    15781 attttaaagc ggatgtagaa agaataaaat agtttgagga ggtaagttta ggtgatatat
    15841 aactatggtt ttttagacga aaacacaaaa aaagaaataa gaagaaagat attaaaagca
    15901 atatcaatac ctggttatca agttcccttt ggttcaagag agttgcctat agctaaggga
    15961 tggggtactg gaggtttaca attaacacta tcattaattg gtaaaaatga tgtagtaaaa
    16021 gtaattgacc aaggaagtga tgattctaca aatgcttgta atataaggag ctttattgca
    16081 agtgtaagta atgtagaaac cacaaaagat acattagaag ctacattgat tcaaacgaga
    16141 catagaatac cagaagaaaa acttaaatct aatcaaatac taatatttca agtaccaata
    16201 ccagaaaccc ttagaatagt agaacctagt gaagtagaaa caagaagaat gcattcagaa
    16261 gaagattata gtaggatgtg ggtatatctt tatgaagata tagttagatt taatgatata
    16321 tctatagcag ttgaatatcc atgtaaagta aatgatagat atttaatgaa tccatcacct
    16381 atacctagat ttgatataaa aaaattaaat atgtcagaca atttattctt gtttggtgca
    16441 ggtagagaaa aaagaattta tgctattcct ccatatacaa aggtagaacc tttagagttt
    16501 gaggattata agtttgaaga ggaaaagttt gaaggaaaat actgtagttt atgtaacagt
    16561 acaaatacat ttttagatga agtatatgac agtgacacta atgaaaagta ctatagttgc
    16621 tcagatacaa gttattgtga aaaagttaga ttaaaaaata acagtataga tgtgacaata
    16681 ggaggtactt ggaatgagta aaataaatga ctctaaaaga ttagaaaata tagacaagca
    16741 agtattaata ggaagaaata tcaatcttat ttatggagat gggtgtgaaa agtgttttga
    16801 aagcacagga tatgaaagca ataatatatg taagtattgt ggttctatag ttgcatgtaa
    16861 taatgtaaat ttagaacttt atgaaggaga agtccttgga attgtaggtg aatctggttc
    16921 aggaaaaagc actttattaa aaatactgtt tttccaagaa catgcaaata gtggagaagt
    16981 atatatatcc cattacaata aagacacaaa tatactagag ctttcagacc aaaaaaagag
    17041 atacataaag aatcatttta tgggtattgt gtatcaaaat ccccaccttg gtttaaattt
    17101 agactttagt agtggtggta atatagcaga aaaattattg atggcaaatt tatataatgt
    17161 tgaaaaaata agagatagag gcaaagaact tttaataaaa actaatatac ctgtagaaag
    17221 aatagaccat aaaccaaaat actttagtgg gggaatgcaa caacgagttc aaatagcaaa
    17281 agctctttca aataaccctc caatattgct attagatgag gttacaacag gtttagatgt
    17341 ttctgtgcaa gcggaagtat tggatttaat aagagaaatc caaagagaac ttaagatttc
    17401 tatgatagtt gtatcccatg actttgatgt tataaagatg cttgctgata ggacaattgt
    17461 aatgaaaaat ggaagtgtaa ttgagtctgg tcttacagac caaataatgg aggacccaca
    17521 acacccatat actcagcaac ttataaattc attgctataa atatcgtttg taaataagca
    17581 aaatgggggt ttttaataaa atgaaagaaa ctatacttga agtaaataat tttagtaaag
    17641 actttgagtt acatgcattg aacaagacta taacggcatg tagtaatata agttttgaag
    17701 tttcaaaagg tgagttttta ggaataatag gtaaatctgg agcaggtaag tctaccatat
    17761 taaaaagtat ctataaaaca tatataccaa caactggaga aataattttt aattcagaaa
    17821 tatatggtaa tgtagacctg gctaaaattg gagaccgaga aatgataaat cttagaaaga
    17881 aagaaatagg atatgtatca cagtttttaa agacacttcc acgaataact gcaattgaac
    17941 tggtagtaca ttctttaatt gagagtgggt ttgaaaaaga ttgttcgtat gatatggcaa
    18001 aagatatact taaccaattt gaaataaagg aaaatttatg ggatgcgtat ccaaataatt
    18061 tttctggtgg agaaaaatta agattaaatt tagctcaagc tatggttaaa aagcctagac
    18121 ttttgttact tgatgaacca actgcatcac ttgataatca atctaaaata tatgtaaaag
    18181 aaaaaatgtt ggagcttaaa agtcaaggaa ctacaatgat aggaattttt catgatatag
    18241 agtttatgga aactgttata gataagacct ttactatgac aaaagggact ataagtgaaa
    18301 gtggggtagc ctagatgata aaactaattc ataatgcaaa tattgtatta gaagatatga
    18361 ttttagaaaa tggtcattta atcatagagg gagatacaat aaaaaaagta tcaaatgata
    18421 agattgatta cgcatttgac tttaatgaga tagatgaaat aatagatgca aaaaacttat
    18481 atgtaatacc tggtattata gatattcaca gtgatgccat tgaaaaggaa atagagccga
    18541 gaccaagtac attattacca tttaatatgg cattttatga actagataaa aaactacctg
    18601 caaatggaat aactacagtt tatcattcta tatctttagg ggatggagta ggagttagaa
    18661 gtattgataa ctctttaaag atgattaaaa atattgattc atataaaaat atagatagta
    18721 agagcataaa tcataaagtt catttaagat atgaagtgtt gtattatgaa ggtttagaaa
    18781 aggttttaga actattagat gaaaataaaa tagactactt atcaataatg gaccattctc
    18841 caggacaagg tcagtataca aatccaacat tttataaaga gtatgctact aaagtgtggg
    18901 gagttacaga aaattatgta gatacatggt tagatgattt agtaaatctg catgataatt
    18961 tagattggaa taaaatagct aatattatag gtatagccaa aactaaaaat ataaatgttg
    19021 catcacatga tgatgataca ttagaaaaaa tggatttcat aaaaaatata ggcataaatg
    19081 tatctgaatt tcctataact cttgaagttg caaaacattc aaagaaattg ggtgttctaa
    19141 cttgtttagg tgcaccgaat attgtaagag gtaaatctca taataacaat ttaaaagcaa
    19201 tggatgctat attggaagat tgttgtgaca ttgtgtgttc ggattatctt ccatcagcta
    19261 tgataaagtc tatgtgtata gttgcagaaa gaataaataa tttaaataaa gcagtcagtt
    19321 tatttacctc aaatcctgct aaagcagttg gtatatatga tgaaagaggt agtataaaag
    19381 aaaataaaaa agcagattta gttttagtag atattgatag tgaatatcca aaggttgtaa
    19441 atactattgt aaatggaaaa acagtttata aaagggaggt ataaagattg ctaacagagt
    19501 tacattgtca tacaagtata tcagataata attttactac tgaagaaata ataaaaaaag
    19561 cagcagaaaa gaatataaat atcttagcaa taacaaatca tgacacatta tctggattgg
    19621 atgaagctct tgtgcttgga gagcattatg gaataacaat aataccaggt atagaaattt
    19681 cagcctatga ttataaaaat agaaaaagag ttcatattct tggatataac atagatttaa
    19741 acagtcaaga aataaaaaat ttgtgcaatc caatggtatg tgacagacat aaagcatctg
    19801 ttaagatggt aaataaaatt atagatttag gatacaaaat atctctggaa gatgtaaaga
    19861 aatattcatc aaagactgga atatttaaac aacatataat gaaagctctt atagataaag
    19921 gatatacaga taatatatat tcaaatctat ataaagaatt gtttcataga ggaaacggca
    19981 aggtatatgt atctttaaaa tatgtggatt acagagatgc aataaaagct ataaaaaatt
    20041 ctggtggaat ttgtgtgtta gctcacccgg gtcaaatgga taattttagc gctattgaag
    20101 agatggtaaa agtaggatta gatggaatag aagtatatca tccatctcac aacaaagaac
    20161 tagaaaaaga gtcgctttct tatgctaaga agtataatct agtggtcaca ggaggttcag
    20221 attaccacgg attttattct caacataatt atgaacttgg aagcaagagc ttaaataaag
    20281 aagatttatt taagtttagg ttagctttag gaggggtatt ataaatgtct tttttacaag
    20341 taaaaaatgt aggtaaaagt tatggacaag ttaaggtgtt aaaagacata tctattgata
    20401 ttgaaaaagg agagtttatt tgtttactag gtccaagtgg ttgtggaaag agtacactac
    20461 ttagaataat tgcagggctt gaggataaac atggtggaaa aataataata aatgataagg
    20521 atatgactaa ttcaccacct gaaagtagaa attttggtat agtttttcaa tcatatgcat
    20581 tatttccaaa tatgaatgtt tacaaaaaca tagcctttgg actagaaaat aaaaacataa
    20641 gtaaatctaa tattgataaa aaagttaaag aagttctaga agttgtagaa ttaagtggat
    20701 atgagaaaaa atatccatca caattatcag gtggacaaca acaaagggtt gcattagctc
    20761 gtgcgattgc tttagagcca gactttttac ttcttgatga acctttatct gcattagatg
    20821 caaaagtaag acttaaactt agggagcaga ttagaagttt acatagaaaa ctaggcatta
    20881 caactataat ggtaacgcat gaccaagaag aagctctttg tttagctgat aagatggttg
    20941 ttatgaatag aggagaaata atacaagttg gaacgccaaa ggaggtatat aaaaatccag
    21001 aaacaccttt tgtagctgat tttataggaa caataaattt tatagatgat ggtattaata
    21061 agatagctat tagaccagaa gatataaaag tagagtctaa tagagattct aaagataagg
    21121 atataaaagt gggagaaatt ttagatatcg aatttagagg atttaactat agaattacag
    21181 ttgagtatcg ttctaagcaa atgaaattag atgtagtttc caaagttgca gaacaaatga
    21241 aactgtgcat aggttctaaa ataaacttta agatacctaa agaaggaatt gtacaatata
    21301 aatcagaggg atgtgcttaa tagctagggg tgaataaatg ataacatcaa aaaataaaag
    21361 aagaataagt gatagcacaa tacaaaaata tttcttgatt ttttcggtaa ttgtactaat
    21421 agtatttata ttatttccat tagtatcatt attaaaaaat gcatttatgg atacaagtgg
    21481 aaattttata ggaatttcaa actttagtaa atatgttgaa aatccaagtc ttatagcatc
    21541 atttaaaaat tcaatttttg tatctacagt ctcatcttta atatcactat gtttagcatt
    21601 tatatattca tacgcaataa caagaacaaa cataaagttt aaagattttt ttaaaactat
    21661 gggaatgttt ccactttttg caccaactat gttatatggt atttcactta tatacctttt
    21721 tggaaataag ggaatcttta caaccatggg tctggaagta ccactatatg gtccattagg
    21781 aataattatt tctcagattc tatttacatt tccacaagct tttttaatat tatctgtagc
    21841 actttcgatg gctgattata gactttatga agcagcagat agcttgggtg ctagtgaatt
    21901 taagaaattt tgcaaaataa caattcctgg tataaagtac tcattactta gttcattctt
    21961 tgtatccttt atacttgcat ttacagattt tggagcagct aaggtagtcg gaggaaacta
    22021 caatgtactg gctactgaca tatataaaca agtagttgga caatttaaca taccaatggg
    22081 agctactgtt tctatggtaa tgttaattcc tgtactaata gcatttacat tagataagat
    22141 ggcaagtaag aaacaaggta tgaccataac tgcaaagtct atgccatata agattaagga
    22201 aaataagttt agagatagag tatttttaat agtatgtagt tttataacat tgctaatttt
    22261 tattttaata ttcgtttcag ttataggttc agttataaag ttatggccat ataatttaag
    22321 ctttacaatg gaacactata catttaaagg tattttggga agtggaataa aaacatactt
    22381 aagcagtttg aaaatatcaa ctttaacagc aatatttgga actatatttg tattttttag
    22441 tgcttatatg atagaaaagg tggataagtt tcctaaatta agacaaacag cttacctttt
    22501 atcaatgata ccaatggcct tacctggctt agttttaggt atatcatata taactttttt
    22561 taactcagca agtaatccat taaatttttt atatggaagt acagcaatat tagttatagt
    22621 aaatgtagtt catttttatt cagtttgttt tataacctca aattcatctt taaaaatgtt
    22681 ggataaagag tatgaattag ttgcaaaatc cataaatatt ccattctata aagtattttt
    22741 taatataaca gttccaatgt ctataacatc aatacttgaa atagtagtat attattttgt
    22801 aaattcaatg gttactgttt cagcattaat atttttatat acacctcaaa ctcagactgc
    22861 atcaatatca atactaaagt tagatgagat aggatatata ggaacatcag ctgctatggc
    22921 agtattagtg cttttaacaa atataatagt aagacttttg tacgaatttg taacaaagaa
    22981 attaaaaaat aacacacaaa aatggcagca aaaagatgtt gcatagggga ggtaaaaatg
    23041 aaatttaaga agctagtttc tttaggagta ttatcaacac ttttattaag tgtaataaca
    23101 ggatgtagta ctgattcatc aggttcagat tcaaagtctg atagtaagac aaaaggggat
    23161 ttaacagttt acacagcaat tgaagaagat agtatagagc catatttagc tacatttaaa
    23221 gagaaatatc cagatattaa attaaatatt gttagggcat caacaggaga tataacagca
    23281 agattattag cagaaaagga taatcctcaa gcagatgttg tatggggagt tgcagccaca
    23341 agcttattgg ttgcagatga ccaggggatg ttagaaccat atgctccaaa aggttctgaa
    23401 gaaatagagt ctgcatttaa agatgataaa gaagttcctt cttgggttgg gatagatgct
    23461 tggatgacag gtataacagt aaacactaaa gaattaagtg ataagaacat cccagttcct
    23521 agttcttacg aagatttaat aaagccagaa tataaaggtc ttatatctat gcctaatcca
    23581 tcttcttctg gtacagggta cttaacagta tcagccctta tacaaattat gggtgaagaa
    23641 aaagcatggc agtatatgga taaattgcat gagaatattg gtgtatacac tcaatctggt
    23701 tcagctccag ctacatcagc agcttcagga gaatatccta ttggtatatc ttttggatat
    23761 agaggtataa agttaaaaga ggaaggctat ccagtagaag tagtatttcc aaaagaaggt
    23821 tctggatggg atatagaagc aaatgctctt gtgaaaaaag ataatataaa agaagcatca
    23881 aaagtatttt tagattgggc tataagtaaa gatgtaatga acgaatactc taagaactat
    23941 gcagttacta ctatttctac aggaaatcca ataccagaag gattccctaa aaagccatta
    24001 gagcaaatga tagataatga tttaaaatca gcagcaaaaa atagagaaga tatactaaat
    24061 aaatggatat caaaatatga tggtaagaca gaaaaagaga gttaagtaaa actatacaaa
    24121 tggagtaaaa agaaagaatt tttttaagta gattttatta taataatata agttgagaat
    24181 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagtaagta taccattcag
    24241 tttgaatggt atatttttta tttttagaga ttaatttaga aatagatata ctacagttta
    24301 aaatagtata ttttaatttc attattaaag tatagatatt atatataaag taaataaatt
    24361 attgagcgat tattaataag atatgtataa attttaattt gggaacttgt gttatatatc
    24421 atgaaaaaag agaaaaaata aaaggtaaat tgtttcttga taacttgaaa ttggaagtga
    24481 gaatatttaa ctagagaaga attatttaag tttaagtcag ctttaggagg aggaattata
    24541 aatgtctttt ttacaagtaa aaaatgtagg taaaagttat ggacaagtta aggtgctaaa
    24601 agacatatct attgatattg aaaaagggga gtttatttgc ttattaggtc caagtggttg
    24661 tggtaaaagt acattgctta gaataattgc aggacttgag gataaacatg atggaaaaat
    24721 aataataaat gataaggata tgactaactc accacctgaa agtagaaact ttggtattgt
    24781 tttccaatca tatgcactat ttccaaatat gaatgtatat aaaaatatag cctttggatt
    24841 agaaaataaa aatataagta aatctgatat agataagaag gttagagata ttttagaagt
    24901 tgtagaatta agtggatatg ataaaaaata tccatcacaa ttatcaggtg gacaacaaca
    24961 aagggttgca ctagctcgtg cgattgcttt agagccagac tttttgcttc ttgatgaacc
    25021 tttatctgca ttagatgcaa aagtaagact taaacttagg gaacagatta aaagtttaca
    25081 tagaaaacta ggcatcacaa ctataatggt aacacatgac caagaggaag ctctttgttt
    25141 agctgataaa atggttgtta tgaataatgg agaggtaata cagattggaa ctcctaaaga
    25201 agtctataaa agtccaaaaa caccttttgt agctgatttt ataggaacaa taaattttat
    25261 agatgatgat gtgaatatga tagcaattag accagaagat ataaaggtag aatttaatag
    25321 agactttaaa gaaattgatg taaaagtagg agagattttg gatatagaat ttagaggatt
    25381 caactataga attacagttg aatattgttc taagcaaata aagttggacg tagtttcaaa
    25441 ggtagtggaa caaatgaaac tatgtgtagg ttctaaaata aacttcaaga tacctaaaga
    25501 aagaaccata caatataaat cagagggatg tgcttaatag ctagaggtgg ataaatgata
    25561 acaataaaaa ataaaagaag aataagtgat agtaagatac aaaaatgttt tttagttttt
    25621 ttagtgattg cattagtagt atttatatta tttccattaa tatcactatt aaaaaatgca
    25681 tttatggata caagtggaag ttttgtagga atttcaaact ttagtaaata tattgaaaat
    25741 cccactctta tagcatcatt taaaaactca atttttgtat ctacaatttc atccttaata
    25801 tcattatgtt tagcgtttat atattcatat gcaataacaa gaacgaatat aaggtttaaa
    25861 aactttttta aaactatggg catatttccg cttttcgcac cagctatgct atatggtatt
    25921 tcacttatat acctttttgg aaacaaggga atctttacaa ttataggcat agaagtacca
    25981 ctatatggtc cattaggaat aattatttgt caaatactgt ttacatttcc acaggccttt
    26041 ttaatattat ctgtagcact ttcgatggct gattatagac tttatgaagc agcagatagc
    26101 ttaggggcta gtgaatttaa gaagttcttc aaaataacaa ttcctggtat aaagtattca
    26161 ttagttagtt cgttctttgt atcttttata cttgcattta cagattttgg agcagcaaag
    26221 atagttggag gaaactacaa tgtattggct actgacatat ataaacaagt ggtaggtcaa
    26281 tttaacatac caatgggagc tactgtctct atggtaatgt taattcctgt actaatagca
    26341 tttacagtag ataagatagc aagtaaaaag caagggatga ctataactgc aaagtctatg
    26401 ccatataaga ttaaggaaaa taagtttaga gatagagtat ttttaatagc atgtagtttt
    26461 ataactttat taatctttat tttgatattt gtttcagtta taggctcagt tataaaatta
    26521 tggccatatg atttaagctt tacaatggaa cactatacgt ttaaaggtat atcaggaaat
    26581 ggaatcaaaa catatttaag cagtttaaaa atatcaattt taacagcaat atttggaact
    26641 atatttgtat tttttagtgc ttatatgata gaaaaagtgg ataagtttcc tagactgaga
    26701 caaatatctt attttttatc aatgatacca atggcattac ctggtttggc tttaggtata
    26761 tcgtatataa cttttttcaa ttcagcaagt aatccattaa attttttata tggaagtaca
    26821 acaataatgg ttatagccaa tatagttcat ttttattcag tttgctttat aacttcaaat
    26881 tcatctttaa aaatgttgga tagagagtat gaattagttg caaaatctat aaatattcca
    26941 ttttataagg tgttttttta tgtaacactt ccaatggcca taacatcaat acttgaagtg
    27001 gctgtatatt acttcgtaaa ttcaatggtt actgtttcag cattaatatt tttatatata
    27061 cctcaaactc aaactgcatc aatatcgata ctaaagttag aagagatagg gtatataggg
    27121 ccatcagctg ctatggcagt attagtgttt ttaacaaata taatagtaaa acttttgtat
    27181 gaattgacaa caaagaaatt aaaaactaag acacaaaaat ggcaacaaaa agaagttgca
    27241 tagaggaggt aaaaatgaaa tttaggaaat tagtctcttt aggaatatta tcaacacttt
    27301 tattgagtgt aataactgga tgtaataatg cttcatcagg ttcaaattta aagtctaata
    27361 ataagataaa aggagagtta acagtttata cagcaattga agaagatagt atagatacat
    27421 atttaactac atttaaagag aaatatccag atattaaatt gaatgtactt agattatcag
    27481 taggagatat aacagcaaga ttattagcag aaaaagataa tcctaaagca gatgtgttat
    27541 ggggggttgc agctacaagt ttgataattt tagatgacaa agaaatgcta gagccatacg
    27601 ctccgaaagg ctttgataag gtaaatcctg tatttaaaga tgataagaaa gttccttctt
    27661 gggttggaat agatgcttgg atgactggta taacagtaaa tactaaagaa ttaagtgata
    27721 agaacatacc agttcctagt tcttatgaag atttaataaa gccagaatat aaaggtctta
    27781 tatctatgcc taatccagct tcttctggaa caggatattt aacagtatca gaacttatac
    27841 aaattatggg tgaagaaaaa gcatggcagt atatggataa attgcataaa aatattggtg
    27901 tatacactca gtctggttca gcgccagcta tattagcagc ttcaggagaa tatcccattg
    27961 gtatatcttt tggaaataga ggtataaagt taaaaggtga aggttatcca gtagaagtag
    28021 tatttccaaa agaaggttct ggatgggaga tagaatcaaa tgcacttgtg aaaaaagata
    28081 atataaaaga agcatcaaaa gtatttttag attgggctat aagtaaagat gcaatgaatg
    28141 agtattctaa gaattttgca gttactacaa ttgacacggg aaatccggtg ccagaaggat
    28201 ttccaaagaa tccattagaa cagatgatag ataatgattt aaaatcagca gcaagaaata
    28261 gagaggctat actaaataaa tggatagaaa aatatgatgg aaagacagaa aaagagagct
    28321 aagccttgca ataaaattta gtacaaaaag cgcctagaat ataagtttta tatctaggct
    28381 cttttgatat atttatttaa aatgcatatt tctatataaa aataaatagt ttagtacctt
    28441 gattaaatta aataaaaacc ttaaaataaa gagatgtaaa gaaaaactaa aaacgaataa
    28501 aaggtgaatt tatatgataa aagaaataat agtagtagag ggtagagatg acgttacagc
    28561 agttaagaga gcaatagatg cagaacttat aactacaggt ggatttggat ttccaaaagg
    28621 tgtaatggag cgtataaaag ctgctaatga aagacgtggt gttataatat ttacagaccc
    28681 agattttgca ggtgaaaaaa taagaaaaaa aatagcatca gaagttccag gatgtaaaca
    28741 tgcatttcta ccgagagagg aagctaaaaa ggatggagat ataggtattg agaatgctac
    28801 tcctcaaagt ataatagcag ctctgaataa agttagaact gaaaatacag aaaaaagaga
    28861 tgaatttaag caagttgact taattagaaa tggtcttata ggtaatgaag aagcatcact
    28921 tagaagagat gcacttggaa aaatacttgg aattggatat ggaaatgcaa aacaattttt
    28981 aaatagattg aataattatg gagttgaaag agaagaattt atagatgcat taaaacaaat
    29041 ctaaacgtaa tgtttaatgt atgaaaaata tgtatataaa ataaattgtg gaattacggt
    29101 taatttaaga tgaattattt ataatataca ccttatttta aatcaatgta aatacatata
    29161 aataagtata attaataaaa ttaaatatca aaataagtta aaaaggagta aataatggat
    29221 agactatctt cacataatgc aacaaaggaa gtagtacaaa aatataattt taaattttca
    29281 aagtctttag gtcaaaattt cttgatagat agtaatataa ttgataaaat cttatctgga
    29341 gctagaatca caagaggaga taatatcata gaagtaggac ctggtatagg aactcttact
    29401 cgtgagatgg gtaaaatagc agaaaaagta gtggctatag aaatcgatag aaatttaatt
    29461 cctatattga aagatacctt gtcagattta gataatacag aggttgtaaa tcaagatata
    29521 ctaaaagtag acatacagga attagtaaaa gataaattaa atggtgggcc agttaagctt
    29581 gtagcaaatc ttccatatta tataactact ccaatagtta tgaaattttt agaggaagac
    29641 atacctgtaa cagacatagt agtcatggtt caaaaagaag ttgcagatag aatgaatgct
    29701 attccgggta ctaaagatta tggggcatta tctatagctg ttcaatacta ttgtgataca
    29761 gagattgtgg caaaagctcc aagacatatg tttataccac aaccaaatgt agattccaca
    29821 gttataggtc tacatgtaag agataaaaga aaatatgatg ttcataatga agacatattt
    29881 tttaaaacag taaaagcatc ctttggacaa agaagaaaaa ctcttcttaa ttcgttggga
    29941 ggtcttggtt ttcttaataa agatgaaata agagaagtat taaaagaagc taatatagac
    30001 gaaaaaagaa ggggtgaaac tcttagtata gaagagtttt cagttctttc aaatataata
    30061 aatacaaaag tatcttctaa atagaaggta ctttttttaa ttttgttaac atatattgtt
    30121 tagagaaact gtgttaaggg gggattgcag tgggttctaa aagaaagttt aaaagagact
    30181 atataatact agaagctaaa gacataaact ttagatacaa agaacgagta ctgccaaaag
    30241 cttttgcaaa agttgaagta aatgatgaaa aagcaattat tgcattatat gtagaaaatc
    30301 taaaatatgt taaagatggg tataaagcag tagcaataaa agatgaccat ggattaatag
    30361 acttaggaaa tataatttta aatgaacaag gaagaggtga atttgtttta gatttagaag
    30421 attatgatga tgagataaaa ggtattgcat tattgtatga aagaaatata cctttaattg
    30481 gatttaaagg aaataagctt gaaaattatg aggacatatt atttcaagag gtagaggaag
    30541 aagaatatga agaatatgaa gagttggaag aagaagacga agagatagag gataatgagg
    30601 aaagtgatag agacgaaggt agtgaagaag aatatattat agaagaatat gatgaagatg
    30661 agtatgaaga ctattgggaa gatgatgaag atattgacga aggagagtta aaagcaagaa
    30721 cggaagtaga agattctggt gatgaggaaa tagaagagtt caataattat aaggaaatag
    30781 aaaattataa tagggttaaa gtacaggaag aatatataga catagaatat gatgaggaag
    30841 aagaggaaga agaagaagtt tttgaagtaa atagagaaga agataggttt gtttcctcaa
    30901 aacctactaa atcaaagaaa gttcaaaaag atcagaataa tattgaaaaa ataaaaagag
    30961 ctaatggagt aagtcaagga gagagtggaa ataaacaaac cagtacatta cttatgccaa
    31021 gacaaataaa aaaaggtttg aaattattta aagaagtaaa accttttgta acagattata
    31081 tagataagac aagatggtgg aaaatagaga taaatcctac aacattgtgt ggatatacaa
    31141 tgccttattt aggatattta aacgtgttga attacaccat gtatagtgat gcagttttac
    31201 attcatataa atacagacac tatttatttg gagtccagta tgatgaatat aataagagaa
    31261 aacattatat ttatgctatc ccaggaaata gaaatgaaca gccagacaat ggaagcacag
    31321 gatttttaag atatcaagct tgtgataata gaacaaatag tttaggatat tggctatgtt
    31381 ttatagattc tagacaaaga aatatagtta ggtaaaaata ataaaaatga aaaaatttag
    31441 actcaattct tattttactg aaaaaattaa tgaaatgtag tacaaaactt taaaaactat
    31501 tgataatatt taaatattac actacaatat atcttgaaat acctattata ttatggagga
    31561 aaattatgat aaacgagcaa agaattttaa atgagtttct tgaattagta cagatagata
    31621 gtttatcttt aaaagagggg aatgttgcta aagtattagt taaaaaatta gaagaaatag
    31681 gttgttcagt agtaatagac aatgctggtg aaaaagcaaa tggagaaact ggtaatataa
    31741 tagctacatt aaaaggaaat aaagaaggaa agaaaatatt atttagttca catatggata
    31801 cagtaactcc aggtataggg gtaaaaccta tagtggatga agcaaatgga ataataaaaa
    31861 gtgatggtac tacaatatta ggttctgatg ataaagcagg tatagcagct atattagaag
    31921 gcttaagata tataaatgaa aataatatag aacatactga tatacaagtt gtattctcta
    31981 tatgtgagga atgtggatta gttggagcta aaaatttaga ctatggaaaa atagattctg
    32041 aatatgcatt tatattagat agtggtggtt ctccaggtga aataatagta aaagctcctg
    32101 cacaagatgt aataaatgtt aagatattag gaaagactgc tcatgcaggt cttgaacctg
    32161 aagctggaat aagtgctata atggtagctg ccagagcaat agaaaatatg aatttactta
    32221 gaatagacga agaaacaact gctaacatag gaattataaa cggtggaaca gctacaaata
    32281 tagtaacagg agaagttaat ataattgcag aagccagaag tctaaaagaa aataaattag
    32341 atgttcaaac taaacacatg gtagagactt ttgaaaaagc tgcaaaagat tttggagctc
    32401 aaattgagat agatgtcaat agagcatata caccaataga tgttagtgag gattctgaaa
    32461 taataaaact tgcaaagaag gctttttcaa acttaggtat agaaggtcat actgaatcta
    32521 caggaggagg tagtgataca aatatattaa gtaagaatgg tattgaagct ataactttag
    32581 gaattggtat gaaaaatgct catacactaa gtgaacatat agctataaaa gacttatatg
    32641 attctgctaa aatggtagtg gagataataa aggaagcata aatttaaata atagtgtata
    32701 atatatatat cgcctagctt aagctaggtg atttttcatt ttatagaaga ttgataatct
    32761 ctattgaaag taaagagtat acatttgcat ataagagagt gtttaattta tgttcaaatg
    32821 taggttctta ataatattta gattgtttca taagggagga ataaatatgg aaacaaggta
    32881 tgaagaagca aataaaataa caatacaatc aatactttgg aatgtagtat taacaataat
    32941 taaggttata gctggtgtta ttggtaattc tagcgctatg attgcggatg gattacattc
    33001 agcatcagat attataagtt ctataggagt actaataggc aattatgtat cttctagacc
    33061 tggagataga gagcataatt atggtcatga gaaagcagag acattagtat catttgtgtt
    33121 atcaatattg cttattttcg tttctataac aataggtata gaggcaatta aatctttatt
    33181 taacttagat gcattaagtg ttccatcaat attaccatta gtagtatcag ttatttcaat
    33241 cctgataaaa gagtatcaat atagaataac aataaaagtt gctaaaaaga taaattcacc
    33301 agccctaaaa gctgatgctt ggcatcatag gtcagatgct ttatcttcag tagctgcttt
    33361 tataggtata ggtggttcta ttttagggtt taaaccatta gacccaattg cgtcagttgt
    33421 agtggctata tttgtagcta aagtaggaat tagtatactt ataagttctg ttaatgaatt
    33481 gatggatgtt tcagttgatg aagaagagat aaaagaatta aaatttattg tagcagatac
    33541 agaaggtgtt aaaaatcttg gtgatataaa gactagaaaa catggagcta tggcatatgt
    33601 agatttgact atatgtgtgg atgaaaattt gacagttaag caaggtcatg atattgctac
    33661 caagcttgaa aaacatataa taaaacatat ggaatttgta aaaggcataa cagttcacgt
    33721 tgaaccatgt actaattgcc aaggaaataa gtgtaataat taattaaaac ttaaaaaaaa
    33781 gtgtaaaaag ttatataatt gttttatgat tataaactct aggggtggtt aaatgagcaa
    33841 gaaaagaaaa ctaaagaaaa aagctgcaat attaatagga gtagttgttt tctttataat
    33901 atactttata tcctttagaa taggaataaa tatggaaaag gataaaagtg aagtgccaaa
    33961 gaaggtagaa aaagaagaag aagataatag gtctttaagg actcaaataa aagagaagaa
    34021 gaaggtttat atatcagata aaaatataga aaacataaaa attgaagaag atatgtggga
    34081 tgaaataaag ttatttttct cagattttaa gaaagtaaga aatactactt cttcttacga
    34141 agcagtttat gaaggttatt ctgatgatgg agttaaattt tctactgacc ttaacttttt
    34201 tagagtatat actgtaaata aagaagaata ttataaagta ccagttagtt ctaaggatgc
    34261 ttttaagaaa cttttagatg agagcatata tacttcgttt gaccttttaa gtcattataa
    34321 agattggaaa gaggttacta taactcatgg aaaagaaatt aaaaaagttc atagatggaa
    34381 atttgatgat ttagcacata agatgatttc aaagagggtt gtaggaaaag ttcaacctga
    34441 gaaaagtaag gaaagaagtg actataattt tactataaat ataaaaggtg ataattttga
    34501 tgtaaaagtt gatacaatgg gaaaagatta tgtaaaagtt acttctaaga aaaatgtatc
    34561 ttactatgag gttcatacaa gtttatttga ttaccttaaa aatgagatat ttgaaattgg
    34621 aaagaaataa gactttaaga aggctatcaa attagatatt tgatagccta aattatttta
    34681 tatattattt ttcaaccaac aagtctgcaa ccttataaat tggaccagca ccagcagtta
    34741 ttactaaatc attatcctta acattttcac gtaagtattt tactatatct tcaaattctt
    34801 ttatatagat ggcatctaca ttgttttgat ataatttctc aaccaagtcc ttagaatgta
    34861 tatctcctgg gtctttttcc ctagcagcat atatatctgt tatgataact ttatcagcag
    34921 catagaatgc ctctgaaaat tcacctaaaa gagattttgt tctagtataa gtatgtggct
    34981 ggaagataca ccataaagtt gattttttta attttttggc agcagctaaa gttgctttta
    35041 actcagttgg atgatgtgca taatcatcta ctactaaagc attcttataa taccctttag
    35101 tttcaaatct tcttccgaca cctttataaa tttttatatt ttttcttata gtctctaagt
    35161 caataccaga aacaagagct gccattatgg cagaagaagc attgtatatg ttatgaagac
    35221 catatacaga aagttcaaat tctcctaaat ctcttccttc atattctatt ctaaatattc
    35281 catgaccact attgtcaaag tgaatatctt ttattacagc atcattgtca gaatctctgc
    35341 cgtattttat aatagtagct ttaacatcat acaatatgtc atcaatattt tcatcatcgc
    35401 catttattat gaaatagcct tctgggggta ataattttcc aaatttgtta aaagaagctt
    35461 tgatttcatc tattccagag aaataatcaa gatggtcttc ttcaacattt aaaactatag
    35521 atatctttgg gttaaaatta aggaaactat ctacatattc acatgcttca gttataaagt
    35581 ggttggaatt acctatctta acattaccac ctatcatact taagttacca ccaactagta
    35641 tagttgggtc taagtctgca tattcaaaga ttgtagataa catagatgtt gtagaagttt
    35701 ttccatgtgt tccagaaact gctatagaat ttttatattc cctcatgatt tggcctaaaa
    35761 aagtcgctct gttcattacc aatttatttt tttctttagc agccacaagt tcttcattgt
    35821 ctggatgaac tgctgctgta taaacaacca tatttacatc atctgatata ttatcttttt
    35881 tctgaccaat ataaatagtt gccccttggt ccctaagttt gtctaatagg tatgattcgt
    35941 ttgaatccga acctgaaact tgataacctt tattgataca tatttcagct aatgcactca
    36001 tgctgatacc gccaattcct ataaagtgta tattcataat aaaaccacct ttctttttaa
    36061 ttaagtccta caatatgaac tatttctgtg gtataataat ataatgttca atattttata
    36121 attaatatgc atataatatt atatgtattt tatttttgaa tataataaaa cataagtaat
    36181 attataccac aataagaatt gttatgcatt agttagttta acgttatata aaggtacttg
    36241 taggaggaaa taataatgaa atttaaaaga acagagagaa ttggagcaat agttaaaata
    36301 ctgtctgata atccaaataa aatatatact ttaagttatt ttacaaatca atttaatgca
    36361 gcaaaatcaa caataagtga agatttatta gttgtaaaaa atgtatttga gaagctacat
    36421 ttaggtaaag ttataactat ttcaggagca gcaggaggag taaaatatat acctaaaact
    36481 tctatagcag aaaatgcaga gttcttaatg gaattatgcg agagaatatg tgacaagtct
    36541 agaatactat ctggtggttt tttatacttg attgacctaa tttacgaccc tacaatagct
    36601 gctaagatag gaaagatatt cgcatcaaat attgagtatg tagatgcaga ttatgttgtg
    36661 acaatggaaa caaaaggtat acctatggca cttatgactg caaaagctat gaatttacct
    36721 ctagttataa taagaaaaga tataaaggta tcagaaggtc ctacacttag catgacatat
    36781 gtgagtggaa acagttctaa agttgaaagt atgagtttgc caagaaaagc tcttaaacca
    36841 gatagtaaag ttataattat agatgatttc atgaggggtg gaggtactat aaaaggaatg
    36901 gtagacctta tgaatgaatt tggtgcagag gttataggta ctggagtatt catatctaca
    36961 acgaatcctt cagaaaaaat ggtaaaagat tatatttcgt tgattcaatt ggatgttaat
    37021 ggagataaaa tagtagtaga accaaactta aagactttta aagatgaata tagaaatgaa
    37081 gatttagacg aagaagaaga ttcagaattt gaatttgaga tagatgaaga ttaataaaaa
    37141 aaatatttag aaaaatatgt aaaaataaaa agaggatatc cctagttgtt catagaatta
    37201 tttaaaaaac agtttatatt aatttattcc agcgataata attttaatat aaattaattt
    37261 ctgtcaaaat caaaggggga tattttggta tgaagataac tgacgtaaga gtaagaaaat
    37321 taacagaaga aggtaagatg aaatgtatag tttcaataac tttcgataat ttgtttgtag
    37381 tgcatgatat aaaagttata gaaggccata atgggctatt tatagcaatg cctagcagaa
    37441 aagtaggcga aggaaatttc agagatatag ctcatccaat aaatgcagaa atgagacaag
    37501 ttttagaaga tgcagtttta caagcgtatc atgaagcatt agttcaatgg gaagtagctg
    37561 ctgaataata ataaatatat aaaatggaga gagcccgaac atttaggctc tttttaaatg
    37621 tagaatttct attaaaatat gtacttttta gtaaaaaagt gctatattat tagtgataat
    37681 gtatatgttg tatacaaatt gttagataaa tgtattataa tgtatattga ttaagagaat
    37741 aatacgaata aaattaaatt agattgaatg gagtgtatca atgaacttta aagctataat
    37801 ccttgctgct ggcaaaggaa caagaatgaa gtctaaatac ccaaaagtta tacataaagt
    37861 atgtggaaaa gaaatggtta accatattat agatgtttca aaaaaatcag gggtaaaaga
    37921 tactgtcgtg attttaggac atgaagctga tgtagttaaa gaaaagcttg cagaagaaat
    37981 tataatagcc atgcaaacag aacaacttgg gacaggacat gctgtaaaga tggctaaaga
    38041 atatattaat gatgaagata caatagttgt actttgtgga gatactccac ttataaaaga
    38101 agagacacta aagagactat ttgaatatca tatagaaaat aaatatcatg ctacagttct
    38161 tacaacaaga gttggaaatc caacaggtta tggtagaata ataagagata agaagggtga
    38221 ccttcttaaa atagtggaac aaaaagatgc aaattcagaa gaaaagatga taagtgagat
    38281 aaactctgga atttattgtt ttaatggaaa gagtcttaga gaagcactag atttgctaaa
    38341 taataacaat tcacaaggtg aatattattt aactgatact gctaaaataa tgagagataa
    38401 aggattaaaa gttggtgcat ttgcaggttc aactattgaa gaacttatgg gcgttaactc
    38461 aagagtagaa ttatcaaagg cagaagaaat tatgagacga agaataaatg aatctcatat
    38521 ggtaaatgga gtaactataa tagacactaa ctctacctat atagaatctg atgttatgat
    38581 aggcaatgat actataatat atccaggtgt tatgttacaa ggaaaaacaa ggataggttc
    38641 agattgtata attggaatga attcatctat aacaaattca gaaataggtg atggtacaga
    38701 aataaaaaat tcaactataa ttgatagtaa ggttggagaa aattctactg ttgggccata
    38761 tgcatactta agaccaaaga gtgacttagg aaataatgta aaaataggcg attttgtaga
    38821 agtaaaaaat gctattatag aagatggctc aaaagcatct cacctttctt atataggaga
    38881 tgcacatgtc ggtaaaaatg taaatattgg atgtggagta gtatttgtaa actatgatgg
    38941 taaaaataaa tttaaatcaa tagtaaaaga caatgcgttt ataggttcaa attctaattt
    39001 agttgcacca gtagttgtag aagaaaaagg atatatagca acaggttcaa caataacaca
    39061 tgatgtccca gatggtgctt tggcaatagc aagagaaaga caagttatca aagaaggatg
    39121 ggtagaaaaa aagaatcaaa aagacgacca aagtaagtaa ttatgtagtt tattattttg
    39181 gctaaaaaat ttagctcgaa attattataa attatattca ttagtatttt aaaattttca
    39241 ggagggttac ataaatgaat actagcggaa gcgagattaa aattattgca ggtaattcat
    39301 cgaaagagtt agcacaaaaa atagctgact atataggtgt atcagtacta gattgtgaag
    39361 taggtacatt tagtgatggt gaaatatgtg taaacatgaa tgagacagta agaggttgtg
    39421 atgtgtttgt agtgcaatct actaacagcc cagtaaatga taacttaatg gagttattaa
    39481 tcttaattga tgcgttaaag agagcatcag caggtagaat aacagcagtt attccttact
    39541 atggatatgc aagacaagac agaaaggcta aggcaagaga tccaatcact gctaaattag
    39601 ttgcaaattt aataactgct gctggagcag atagagtatt aacaatggat ttacatgcag
    39661 ctcaaataca aggatatttt gatataccat tagaccactt actaggtgga acaatattag
    39721 caaattattt taatgaaaag aaaatagaag atttagtagt agtatctcct gacttaggaa
    39781 gtgttactag atcaagaaaa tttgctaata ctttaaatgg agaagttcca atagctataa
    39841 tagataaaag aagaccgaaa gctaatgtat gcgaagttat gaacttaata ggtgatgtta
    39901 aaggtaaaaa tgttatacta ttagacgata tgatagatac agcaggaaca atagttaatg
    39961 ctgcaaatgc acttaaagag tttggagcaa aagatgttta tgcttgttgt actcatggtg
    40021 tgttatcagg tccagctatt gaaagaattg ctaattcaga aataagtgag ttaatagttc
    40081 ttgatactat acaacttcct gaagagaaaa gaatagataa gataaagata aaaacagtag
    40141 ctcctttatt tggagatgct ataagaatga tattctctaa tgaatcagta agtaaattat
    40201 tctaatttat tgtatttatg agagttgttt aaaaggatat aaaccttttg agcaactctt
    40261 ttttgatttt ataattatgg aatgttattt ttttcttgtt agaaaggtat atattattgt
    40321 aaaaagagat tatagtttaa aattatgtat atttaagtaa aataaactta ataaagggca
    40381 tattattcaa aatattatga atataaaaaa tattacaaaa aagtattgaa aaaattcaga
    40441 ttatgtagta ttatgtaaat ataaataaag aaagaaagag gtgtttccaa tgggaaggtt
    40501 agaagatgaa cccctaataa ttaaatgatt cagtcacaat ctaaagtgaa tataaaaatc
    40561 tattgaaagt ttatttataa gaattgaaaa tagtatctat tctatcttta gtgtttgtga
    40621 gagggttctg gattatataa gattactgta aagaagtaat tttgcacaat aagcatatct
    40681 gtaaatgaag agtttcgttt atataatgat tgtggctgaa tcataactat aatagataga
    40741 atgtttataa tagatgattt tataaaatac tatattgtaa tagcttttag ctttcaaata
    40801 aatattagca aaggatggaa atgacaatga taaaactaaa tgataattta taaatatctc
    40861 cttacgatat ataataattg tagggagatg tttctttttt ttgtagagta taatttttct
    40921 agaaattctg tttcaatatg taaaaagttg gtatatataa atagtataaa cagtgttgct
    40981 aaaatatggt agatattata taatataaat ggatatgtat atacattatg agttatataa
    41041 tttgaattta ataaaataga aacgttgatt tatgttctgt aatgtgggca ccttggagca
    41101 tattgagtta gtggtgcaac cggctatgaa attgtattta tttataaata ctattatttt
    41161 catagctttt tttatttaaa aaatggttat atgttagaaa taaatttggt tagtacgttt
    41221 aaataatttt acttagattt ttaaccagat atattataag taagtattaa ttaaaaaatt
    41281 taagggggaa taaaaatgaa gttaaaaaag aataaaaaag gtttcacttt agtggaatta
    41341 ttggtagtaa ttgcaattat aggtatatta gcagtagtgg cagttccagc tttatttagt
    41401 aatataaaca aggctaaggt agcaagtgtt gagtctgatt atagctcagt taagagtgca
    41461 gcattatctt attattcaga tactaataag ataccagtta caacaactga taaagctggt
    41521 ttaactattt tagagactta tatggagtct ttgcctgata aagctgatat aggtggaaaa
    41581 tataaattga ttaaagttgg taataaattg gtattacaga taggtacaga tactgaagga
    41641 gttacattaa cagaagcaca atcagcaaaa ttattgagtg atataggtaa agataaaata
    41701 tatacaggtg ttacaggaga taattttgga gacgaattaa cagatactac aaaaatagat
    41761 aataaagctc tatatatagt acttatagat aatactgtga tggactcaac aaaataatta
    41821 aaactatatt tttataaaga atattgtaaa tttaatgaaa aatataatat aaaagacagg
    41881 aagaaaatct tcctgttttt ttatactatt ttctttaatg ttcaattatg aatgatgata
    41941 taattgagat agtaaaggag tgaaacctgt ggctaaaaaa gttagaatag gagacaaact
    42001 ggtcgaaaaa ggatatataa cagaagaaca gcttaaatgg gcactatcag aacaaaaaaa
    42061 cagtggaaaa agactaggag aatttctagt acaagaaggg cttatagata gtaacttact
    42121 aataagtgta ttaaaagaac tcttggatat agaaagtata tttttagaag ggacagaaat
    42181 agacacttta gctacaaaaa tggttccaga aaatatatgc aaaagatata ctgtatttcc
    42241 atttaaaatt gatggaaata aaatatgtct agccatgtca gacccacaag atagagaagc
    42301 agttcaagat gtaaggcgta tgtctggtaa agacgtagag atatttatat caagtacaga
    42361 agatataaac aaagcaatag gtcatgctta tgcacattca gaaataaata aggcaatgac
    42421 tgagtacaac aaaaatagaa ctggtggagt aagagaaaca gtaatacttg aagaagatgt
    42481 aaatgctgct ccaatagtac gattagtaaa taacatacta gaaaatgctg ttagaatgga
    42541 agcaagtgat atacatatag aacaaagtga aaattacatg cgtgttaggt ttaggataga
    42601 tggaatgtta agagaatata tgaggatgaa ctcagcacca tataaggcag taatcagccg
    42661 tatcaagata atgtcagata taaatatatc tgaaaagaga ataccacagg atggtagaat
    42721 ctatttaaaa gtggataata aacccataga ttttagggtt tctacaatgc caactaacag
    42781 ggatgaaaag atagctatga gggttcttga taagagtaat tttatggtta gtaaagaagt
    42841 actaggaata gacgaacatg gctctaagat atatgatgaa ttgataaaca ctccttatgg
    42901 tcttatattg gttgttggcc ctactggaag tgggaaaacc actacattgt attcaatgtt
    42961 aaatcaacta aatacagaaa atagaaatct attaacaata gaagacccta tagagtatga
    43021 attacctggt gtaaatcaat ctcaaataaa tgaaaaggca gggcttactt tcgccagcgg
    43081 acttagagct tttatgcgtc aagacccaga tataataatg gttggagaga taagagatac
    43141 tgagacagca gaaatagcaa ttagagcttc tcttacagga catttagttt taagtacatt
    43201 acatgcaaat acagcagttg gagcaatttc acgtctttta gatatggatg tggaaagttt
    43261 tttaataact tcatctgttt taggtgtaat atctcaaaga cttacacgaa aaatatgtga
    43321 gcattgtaag gtttcatatg aagcagatat tggagaaaaa aaggctcttg gtattgatgt
    43381 aaatgaatca gtaactattt atagaggaaa aggttgtgaa aggtgtaata atactggata
    43441 taaaggacgt ctaggtatat ttgaaatgct tgaaattact ccagaaataa aagagttaat
    43501 agattcatct gcaaatcaaa gagaaatttt aaagatggct agaaaacaag gtatggttag
    43561 cttaaaagag gatatagtta aaaaagtttt aaatggaaag acaacagtag aagaaatgat
    43621 tagaataatt ttgatgactg attaaaggat gatattatga aaagttttaa atacacagta
    43681 ataaaagagg acggaaaaaa gcaaactgat gtgattgaag ccaacgattt taatgaagct
    43741 agaaatcttc ttagaaagag aaatcttaga gttattgaaa tcaaagaaag caaaaataaa
    43801 aatataagat tatctagtag aaagagaaaa aaagacttag gagctgacca gataagccac
    43861 ttttgtagac aatttgctat tatagtatct tctggtataa atagtatttc tggattagaa
    43921 acattagcta gaaggtcaac taacattaca ttaagagaag aaattaatcg tatagtagca
    43981 gaaataaaag taggttctac tattgcagat tcaatgcttt catcaaaatc aaagtttcca
    44041 aagttacttg gtgctatggt agctacaggt gaagctacag gtaaattaga tgaagtatta
    44101 aaaagtatgg cttcatttta tgcaagtgaa cacagggtta aacaaaagct tagaaatgct
    44161 gcaacatatc cattaatagt tttgatttct tcatttataa tgatttttat atttacaaca
    44221 tttatggtgc ctaagatgat taattcaatt acaacagtag gagcaactct tccacttata
    44281 acaagaattg ttatggggtt tggtatgttt atgaagaagt tttggataat agtactttta
    44341 gttattatat tatttatcta tcagtttaaa aaatatctaa aaacgtcagt tggaagggct
    44401 cataaagata gagttataaa taagatacct gttttaggaa aaggaataaa ttgtatggta
    44461 gctacacgat tttcaagagc attgtatcta tttgtatcaa caggataccc attagttcaa
    44521 gggcttgatt atattataga tagtgtaaat aacactatgg cagagaagat tttagcatca
    44581 gctaaagatg gaataacaag aggagaaggc ttagcagaaa atttagagag atatacgtat
    44641 tttgactctg tgttggtaca gatgatagct ataggggagc agacagggga attagagaat
    44701 ataagtagac agatggcaga attttatgag tatgaatccg agatttattt aaataggatg
    44761 gcctcaatga tagagcctgt tttaataatt gcagttggta ttatagttgc gatactggtt
    44821 gtatcaatat tcatgccaat gcttagtata tatgatgcca tgtaggaggg aaaggacaaa
    44881 tgaacaaggt ttatgtatca tattatggcg attctatttc catagttgag ggcagatata
    44941 aacacgataa ggataagttt tatgtaaaaa attttaatgt gttttcaata gaggatattg
    45001 cacaagattt ttctaaggaa aagtatgctc tattgagata cgcattagaa aatgttaagt
    45061 taaaatcaag acatgtagtt ttttgtttaa atacaagaga tgtaatttta aaaccccaaa
    45121 aacttcctaa tatggacaaa aaggatttag acggatttat gaagctagag atggacgaaa
    45181 tgatggcttt ggagtcagat gaatatgtat tttcgtatga agtatctagt gaaacacctg
    45241 ataattcaga tggcgatgaa tcaagcttaa atcttattat tggagctata caaaaggaag
    45301 aggtaaatgc aattgtagag ataatgcatg aatttgattt agtattagat agaatagata
    45361 cattatcaac agcttactta agaatgctta aaaatttaga ctacaatgat ataatggtaa
    45421 ccaatataag tgacaatagc acaattataa atatatataa gaaagatact ttatttatta
    45481 gtgataatat acctataaag ataactgcta aagatatgga catgcaatta ttaaatgtag
    45541 tagaagaatc aaaaggtctt atgaattact attcgtcaag aaactttggt aaggttacgg
    45601 atactatatt aatattagga aaaagatatt ccaataaatt aatttatgaa acttttagct
    45661 ctgcattttc aagctatgtt tctcaaggcc ttactgagat aatggatata tctgatatga
    45721 taactggaga tatagatgaa gatgatataa attcaattgt agagctttta ggaagtatga
    45781 tagaacctaa agggaaaaga gattttgaaa atataaactt tttaccaatg gatatactta
    45841 gaaaacagat aaaagctaaa caaatgaaaa acatactgaa aattgctcca atagttattt
    45901 tgattcttgc tataccttat ttatgtttaa ttttttcaaa taatttagca aataaggaac
    45961 ttgaaaatgt taatggtgaa ataagaaata ttgaatctgc atattatcag attggaaata
    46021 tagaaaaaat gatacagagt aaaagcgaag aaataaaggt atatgatatg ttaattggga
    46081 aaaaggtaaa gtgggaaaat ttattggatg atatagataa aaatactcca agtaaagttg
    46141 aactaacaag tttatcaaca acttatcaaa atcttggaac taataataaa gctgataatt
    46201 caaaaagtaa taagactaca acagataaat caagtaacaa tgaaacaaaa tcaagtacga
    46261 aaagtgatag taatggagat aaaacagata atgatttaaa tgaagataag actacaaatg
    46321 tgtcagaagc attaactgga aatagtgaga ctaatagtaa taacacggat aaagatgata
    46381 aaactccatt gtatgataag ataccaaatg ttataaatat agaaggaaat gctaaagatt
    46441 cctcttatgt aggtcaattt ttatataaat taaagggatt atactatttc aaggatgtta
    46501 aattacatag cataaaaagt aatgcagaag attctaacta tacatttagt atgacattat
    46561 acctgaaaga aggtgtatta actaatgaat aaagatatat ttaaaaaaga tataaaagat
    46621 atatttaaaa tggaagttag tctaaaaggg attcttataa aggttataat tttattagct
    46681 attttatcat atggttattt tggactttac cctcaatatc agaaatatat tgctacaaaa
    46741 aattctgtaa aacaggctac tgaaaaatta aatatatata aagagaaaac tgcacttatg
    46801 ccaaaatatg aaaaacaact tgaggaagtt gaaagtgaat taaaagaaaa aagcaaaaat
    46861 cttttatatg atatgcaaga tggtcttttt ttaataggtt tagataaaaa aatgaaggct
    46921 tataatattg acttaaccag ttatgacgtt ggaaatgtta aagaatacga acatttttat
    46981 ggtatatcaa caacaataag tgttagaggt gattatagaa atgtcaaaaa attaatggct
    47041 tatttagaag aacaaaaaaa tgttactcaa atactagatt atacaatgac ttcatatata
    47101 gaaccagaaa aagaagagga agaaacaaca aacaataagg atttaattaa aaatgtgaac
    47161 ataaataaag atacaaaaat atactgggtg gaaggcgaaa ataagtggca tttatataag
    47221 gattgtccat tgataaaaga gggagagatt ataaaaaatg acaaactttt agataggtct
    47281 actaattatc tatgtaatac ttgtgtactt agagctgcaa cagaaaaaga agcaaaaaaa
    47341 gaagtcgtag ctccaaaggc agaaggtgta gtagatgcta catttaaatt tataatgtac
    47401 actaaagaga atactacaat gatacttgat acagataatc catcttcatg gagaccagga
    47461 aaatataatc catttaagtc tagtgcgaaa taaagctaag taagctttaa agataggtga
    47521 tgagtttgta caaaaataat gaaaaaggtc ttactttatt agaagtaata atagctgttt
    47581 ttatactgac aatagtttta agtatttctt ataaagtgtt caatggtata acatcagcag
    47641 taaaaaagca acagattatt acagatgctc aagtaaatat taatctaatt aataagtatc
    47701 taaatagaga tttggaaaac tgtaaagagc taactaaaac tggttcaggt aataattatg
    47761 aatacaatat agagatgcca gataatgttg taaaatatga agtttctata gaaactaaaa
    47821 aaaatacaga ggtatattct gtaacgagaa tccaaaacaa tacaattgat acagaaaatg
    47881 aagttagaga agagataatc tataatcaac cactagttca aaataataaa gaaatgaagg
    47941 aaacaccatt taaaatagaa aagcaaactg gtaaatctat ttatactgta agtatatact
    48001 ataatgaatc ggtgcaagaa agtcataaaa atagcaattt aaataacaaa acttatacat
    48061 ttgatgttat gtcaagaata gggtaataag aatttattaa aaaataagta ggttgataaa
    48121 atatgagtaa aaagaatagt gaaagaggat tttccttaat tgaagtgtta gtagctatgg
    48181 ctattatggg aattgtacta tttgcatttt ttaatattat caatactaat aacaaagcaa
    48241 acactaaaaa tgacacagat ataacttcct taaactatgt ccaaagtgaa atagaaaatc
    48301 taagagaaaa gataaaaagt ggagaatttg attttgatag tttagataaa ctagaagatg
    48361 gaactgtcgt atatgaaaaa ttaatagata aatcaaaaaa agttgtatat gataaagttc
    48421 ttagtgaagg tgatgtgagc ttatatgata ctccatatga aaagattaca acaataaaag
    48481 atgaagatgg taatttaatc gacaaaggaa atattacaaa taagataaag actattgtag
    48541 aagataaaag tggtcagata tataaaatag ctgtaactgg gaaaagcatg aatgactatt
    48601 caagtaaaaa agaggttaaa attgtaactg aaatatttaa agataaataa ttttacttgg
    48661 gggtgagatt ttgaggaagt ggaataaatt taaaagtgaa agaggggcag ctttagtttt
    48721 agttttggtt gtagtagcat tgctttctat tgttggattg atattttcaa atcagatagc
    48781 taatagaata aaatctacaa agactactaa tgaaggtata caagcaaagt atttagcaga
    48841 gacatgtgtg gaaaatagta ttgataaagc atatgaaaaa ttgtatgatg agcttgagaa
    48901 aatggataat gagtttaaaa gtgaaaatca agaaaaatcc atatctaggt caaaattaag
    48961 aaatatatca gatgaagatt ttaataatca agatgaggaa aatattgaag cagaaagact
    49021 tggatatatg aataatataa atttctattt aaataaagct tcttcagact tggaaaaagc
    49081 tagtatggaa ttgaaaaagc tatatgattt agatatgttg gactatagag acattgaata
    49141 tgtagatgca aatattattt cacatagaaa ttctatctta gaaatttgca agaattatac
    49201 ttctggagat attagtaaaa taaatgaata tattctgaaa gaagatatag actcaactac
    49261 attgattgag gcaaagttag tgaataatga tattctttta aaaaagtttt tagaagaaaa
    49321 taaaattgaa aatgaacatt taaattcagc cttttcacat acttataagg cattagataa
    49381 catttctttg gcgatgcaaa atatgattga gtatagacat acgtttcata tagatgagcc
    49441 aaaagtagaa gtatctaatg ggattccaga ctctcaacaa tattatgaac taatacaaaa
    49501 tcctataata aatagtatgg agtatagctg gaatagtaaa tgggatacat tagaaaacct
    49561 attggaaatt ttaccaaatc aaactcaagg atttaattct ttaagagttc atttgagaaa
    49621 taatgtaaga aaatttgaga agttaagcga taatatatct agtggtaaaa aaaatacagc
    49681 aaaaaatttc ttaaagtata aagagttatt gtatgaaata tcagaccaat gtaatcaact
    49741 aaaaagtatg tcttatgaga aaatacctgt taaatatgat aacatggctt tgataacaac
    49801 ttttgactat atacaaaatg agcttttagc agagataaaa tgcagattaa aagaattaaa
    49861 accacaagaa attgataaaa cagaaggtat tactataaaa ataccttttt ataaggctga
    49921 ctacgacatg acaaaagaag gatggcctaa attaaaagaa aatggttcag gggcggagtt
    49981 aagcttaatg gtaactggtg ataaaaatgg aataaaagaa gtagaagtta cagatggtaa
    50041 aaaaaatatt attggtttag gtgttgaaga aaattcaaat tcaaagtata aggttgacgc
    50101 cattgtaaac tttaatctaa atatagatac aaatgtagtt ggaaattatg atataaaaga
    50161 taaaattttg attaatcatg atataagttc atacaaaaaa gtaaactaaa tataagaagc
    50221 aacttagtat aaataaataa taaaataatt ttattattta ctactaaaaa aataagtaaa
    50281 ctaatagaag atatggagag taaatattat gtatatttat gatttattag agcaaggtat
    50341 aagactaaac gcatcagata tacacataac agttggaaca aatcctgtag ctagagtaaa
    50401 aggtggattt gtaaaactaa gtgaacaaat attgacatca gaagtaacaa tgcaaatggc
    50461 aaaagacata gcaggggaat ctatgtttaa agtaatagaa gaacatggag aagctgattt
    50521 ttcagcttct ctaaaaacag gagaaagatt tagggtaaat gcatataggc aaaaaggaaa
    50581 ttacgcaata gcaataagga ctataacagc agaaattcca acatttgaaa aattaggcct
    50641 tccagagagt ataaaatctt ttactgaaaa gcataaagga ttagttttag ttacaggtcc
    50701 aacaggaagt ggtaaaagta caacacttgc aagtatgata aatataataa atgaaaaaca
    50761 acaaaaacat ataattacac ttgaagaccc gatagaatat gtacatcatc ataaacaaag
    50821 tttagtaaat caaagagaag taggaactga tacagaaagt tttcattcag cccttagagc
    50881 tattttgcgt caggacccag atgtaatact aataggagag atgagagacc ctgaaactgt
    50941 ttcaatagca ttaacagcag cagaaacagg tcatttagta ttttcaacac tacatacagt
    51001 aggggcagct aagacaattg atagaatagt agacatgttt caaccatccc aacagcaaca
    51061 aataaaaaca cagttatcta cagtttgtga aggtgtggta tcacagcagt tacttcctac
    51121 agcagatggg aaaggcagaa tagcagcaat tgaattgatg tttgctactc cagcaataaa
    51181 aaacctgata agagaaggca aaacatatca aattccaaac atgatacaaa ctggagttaa
    51241 agtaggtatg aaaactatgg accaagattt aatggaactt tataaaaatg gaaagattac
    51301 aaaagatatg gcacttagta ggtgtactga ccaagagttt atgacaagaa tgataggtgg
    51361 agtaaactac aatggatatt ataatcgtta atctatatgt atttatagtg ggtatagtgt
    51421 ttggaagttt ctttaacgtc tgtattttta ggattccaga aggtatatct atttctagac
    51481 caccttctca ttgtatgagt tgtaatacta gactaaagcc aatagactta ataccagtgc
    51541 taagctattt aatgagtggc agaaagtgta gatattgtta tgagaaaatt tctagtagat
    51601 atgctatagt ggaattaatt acaggacttt tatttttaac agtatttatg ttatatggaa
    51661 tatctacatc aactatttat tatttggttc ttgtgtcttt actgattata attacattta
    51721 tagatattga ccactttata ataccagata aaatattggt ttttggagct atattttcta
    51781 tgatttttaa ccttttattt aaagaaattc cagttaagga ttcaattcta ggtgttttta
    51841 tttgtggagg aagtgtctgg attatagttc ttttgattga atttgtaata aaaaaagagt
    51901 gtatgggtgg aggagatatc aaactatttg caatgctagg tttttacatg ggtgttaaaa
    51961 atggtctttt gacagcctta ctaagcgttt atgtgggagc agtctatgga atctgtgtaa
    52021 taattataag tagaataaaa ggaaaggaat ataattctgt tataccatat ggacctttta
    52081 tatctattgg agcattaata acaatcttat ctggaaagca attacttgaa ctttattttt
    52141 ctatggttat atagatgata gtcaagttaa ataaagtttt aatttaaaaa aatgaattaa
    52201 agtatttaat aaggacaata ttattattaa ccctattact aaagtgctac aaactatgga
    52261 gtatataata ataatttcct tattttttgc acatttgata aataaatctc tatgtgtttt
    52321 ttgtgataga gaaaagaaaa atttatataa tacgtttttt atttacataa taactgtagt
    52381 tttatctgta ttaatatatc taaaatttgg gattagtgta gaatgtgtac aatatatggt
    52441 tttaattcca tttttaataa ttatatccat tgtagattac tacacaatat atgtatatga
    52501 tataacaata gttagtggta taatagttca aggtataatt tttttggcct ttttcaatat
    52561 agttcaattt gattatataa atcatatatt tggactattg ataggcttta ttatacctta
    52621 tattattgct aaactaacaa aaggattagg ctatggagat gtaggtctct atagcttatg
    52681 ttgctttgca ttgggaaata attatgggtt ttatgtgata gttttatcat ttattttagc
    52741 gtttttgttt tttattattt tatacataac taaaatattt aaagatgtag atataagaaa
    52801 gcaaattcct tttacacctt ttatttcctt gtcaacaatt ttaataatat ttacagagta
    52861 taatatattt aatttttatt ataatttgtt atacaaattt atataacagg aggtaagaaa
    52921 tgtacgtagt agtagggtta ggtaatccag gaaagaagta tgagaagact agacataacg
    52981 ttggatttga tgttatagat attcttgcta aagaatacaa tattagtgtt actaaaataa
    53041 agcataaagc tttgattggt gaagggagag taggtactga aaaagtatta ttagttaaac
    53101 cacaaactta tatgaattta agtggagaaa cattaataga tatatataaa tattataagg
    53161 ttgatttaag taatatagta gtagtatatg atgatataga tttagaagtt ggaaagatac
    53221 gtataagaaa aaaaggaagt ggaggaactc ataatggcat gaaatctata actaagtgcc
    53281 taggttcaaa tgactttcca agagtgagag taggtgtatc taaaccagaa gctggtcaag
    53341 acttagcaga ctttgtactt tctagattta gaaaagaaga atctgataac ataaatgaag
    53401 cattagaaaa agcggcagat gctattgact caataataag agaaaatata gatatgtcta
    53461 tgaacaaata taatggatag tgagagggag atttaaatga atgatgtttt tttatatcct
    53521 ttgcaaaatt caaaagagta taaagatata ataaactgta taaaaaatac aaaaggctca
    53581 ttattggtta atggactttt accagtacaa aaacctcata tatcgtattc tatatttaat
    53641 gatttatcac gacaaatgat ttttataacg agtagtgatt tagaagcaaa gaaagtatac
    53701 gaagatttaa gtttttatat ggaagataaa gttgaatatt taggttttca agatatttat
    53761 ttttatcact tggatgcaaa ggacagaaac gaagaagcga agaaattaaa agtattgtta
    53821 aaactagcaa ataaagaaaa aatcatttta gtaacttcta ttgaggcagt tttaagaaaa
    53881 tacataccaa aacaagtttt gctcgatagt gtatctcatt ataaggtggg agatagctta
    53941 gacttagaaa agttaactga gaaattagtt tcattgggat atgaaagagt ttcaaaaata
    54001 gaggggtttg gtcaatttag tataagaggt ggaattattg atgtattttc acttgaatat
    54061 acaaatccaa ttcgtatgga attatttgat gatgagatag attccatacg aacttttgac
    54121 gtatattcac aaaaatcaat tgataaattg caacaattct ctataactcc atctagggaa
    54181 tttatttatc ccgaaaaaac tacagatgca ttagttaaat taaaaaaaga aacaactaaa
    54241 aatactgatg aaggtacatt tcaaaatata gattatatat caagtaagac ttattttgaa
    54301 ggtgtagaga actatataga ttatatttat ccagaagaaa acaaaagtat atttacttat
    54361 ttagcagatg atgcaatagt atttattaat gatataacga gacttaaaga aagatgcgaa
    54421 aattatataa atgagtttag agaaaattat aaattaaatc ttgaaagagg tctggctatt
    54481 aaaaaccaag gtaaattgtt atatcactat acagatttag agtacttagt aaaagacaag
    54541 tctgtggtat taaactcgct attgccaaag tctataaata acttttctat aaaatctata
    54601 ataaattttg aaagtagaga agttcctaca tttaatggaa aggtagattt gcttgtagaa
    54661 gaattaaatc ggctgaaata taatggatac aaaataattt tagcaacaaa taccttagaa
    54721 agagcaaata aattaggaaa agatttattg gacaaaggct tagaaacgac catttctaaa
    54781 gatagagata ttgaaattaa atcttcacaa gttataattg tacctgcaca cataaacagt
    54841 ggttttcagt ataaatctat aaagtttgtt gttataacag ataacgaaat gataggtgtt
    54901 tataaaagag cgtctaagac tagcaataaa aaagttaaaa agggaaagaa aatagaatcc
    54961 tttttagatt taagtgttgg agactatgta gtccatgaaa atagtggtgt aggtagatac
    55021 acaggaattg aacaaattac agtcaatgct attaaaaaag attatatgaa aatagtatat
    55081 caaggtggag ataatctata tgtgccaata gaccagatgg acaaagtaca aaaatatatt
    55141 ggtgcagaag tagaaaaagt taagttaaat aagcttggaa caaatgagtg gacgaaagca
    55201 aaagcaaaag ttaaaaaaga aattgaagat atgacaaaag acttgattga gctttatgca
    55261 aaacgtgaaa aaatacaagg gtataagttt tcaaaagata caccttggca ggcagagttt
    55321 gaatcgttgt ttccatatca agaaacagaa gaccaattaa aggctataga agaaactaaa
    55381 aaagatatgg aatcaagtaa ggtaatggat aggctagtat gtggagatgt tggttatgga
    55441 aaaacagagg tagctattag aagtattttt aaagcttgta tggaccaaaa acaagttgct
    55501 gtattggttc caacaacaat attggcccaa caacattata atacattcaa agagaggttt
    55561 gaaaattatc ctttgagagt agaagtttta agtaggttta aaacaccaaa acaacaaaaa
    55621 cagattattg aagatgctaa aaaaggatta gtagatattt taattggaac acatagaata
    55681 atatccaaag atataaattt accaaactta ggtcttgtag tcattgatga agaacaaaga
    55741 tttggagtaa aacataaaga agctttaaag aaaattaaat ctacagtaga tgtacttaca
    55801 ctttcagcaa ctcctatacc aagaacatta catatgtctt taagtggaat aagagacatg
    55861 agtgttatag aggaacctcc acaagaaaga catcctgtaa taacatatgt tacagagagt
    55921 aaggaaagcg taattcaaga tgagatagag agagaattaa gtagaggtgg acaggtattt
    55981 tttgtataca atagagttga acacattgaa gaaatggcaa gtatgattca aaaactcgta
    56041 ccagatgcta gagtggctgt tgcacatgga agaatgactt ctaaatcatt agaaaatata
    56101 atcctaggat ttttaaataa agattatgat gtactagtct gtactacaat aattgaaact
    56161 ggtatggaca tatcaaatgc aaatacaatg attatatatg atgcagataa aatgggattg
    56221 gctcaacttt accaattgag aggtagggtt ggaagaagtt caagacaagg atatgcatat
    56281 cttttgtatg aaaaagataa aactttaagt gagatagcgg agaagagatt aaaagctatc
    56341 agagaattta cagagtttgg ttctggtttt aaaatagcaa tgagagacct tgaaataaga
    56401 ggagcaggaa atattttagg ttctcaacaa catggtcata tggcagttat aggttatgat
    56461 ttgtacgtta aaatgcttaa tgatgcaatt aaaaaagtca aaggtgaacc tattgtggag
    56521 gaaattgatg ttgaaataga tttaagtgta aatgcataca ttccagataa ttatatcaaa
    56581 gatgagttga ttaaaattga aatgtataag aaaatagctt ctattgaaaa caaagaagac
    56641 atgttggata tacaagaaga acttgaggat agattctctg atatacctaa accagtccag
    56701 acacttctta caatagctta tataaaatcg ttatgtaaga tattaaaaat agaaaagata
    56761 agacaattaa aagatgaaat attattagtt ccaataacaa agtatagaac taaacagaaa
    56821 ataggttata atatagttac agaattagaa gaattattag aaaagatgtg caaggtaaaa
    56881 taacttgtaa atttaagaag ggatgtttta aatttgaaaa aggtaataac attggtaata
    56941 gctatgattt tggtagtatc tgtgactgct tgcagttcta gtaaaggaga aactgtggca
    57001 acagttgaag gtacaaagat atcatcagat gaatttaaaa aaactatagc tctttataaa
    57061 gattctatgg aacaaactta tggaaaagat atatgggata aggaagttga aaagggtgta
    57121 aaatacaagg acaagtttaa agatttaatt cttgaccaac ttataactac agaagtaata
    57181 tattctcaag ctaaaaaaga taacttactt cctaaaaaag aggatgttga aaaaagcttt
    57241 aaagaattaa aagacgctat gggtaaagat gaaaaatata aagaacaact taagaaatta
    57301 ggtatagatg atgagttttt aagagaccaa caagagaaag atttagcaat gcaaaactat
    57361 caaagtaact ttgctaaaaa gactaaaata tcagatgaag aaatgaaaaa atactatgat
    57421 acacataaag atgaatttaa aaaagatgag gtagaagctt ctcatatttt attgaaaaca
    57481 gtagatgata ataacaagcc actttcagat aaggaaaaag ctgaagctaa gaaaaaggct
    57541 gaagaggctc ttaaagaagt taaatcaggt gaggattttg caaaagtagc aaaaaaatat
    57601 tcacaagata cttcagcaag tgatggtggt aaattaggat tcttttcaag gggtcaaatg
    57661 gttgctgaat ttgaagatgc tgctttttct atgaaaaagg gtgaagtatc tgatttagtt
    57721 gaaactcaat atggatacca cataattaaa gtgacagaca gaataaatga gcaaacatca
    57781 tttgaagatg ctaaagaaac tataaaagac caactgttga aaaataaata tcaagaacaa
    57841 atagaaaaat taactaaaga agctaaggta gaaaaagatg aaaaagtaat aaataaaatc
    57901 acaatctaag acttaaaaaa agagtaatat gcttaaaaaa gctatatctg gataagatat
    57961 agcttttttt gtatattttt cttaacaatg gtaaaaataa acttaaagta atttatggag
    58021 ggattaaaaa atatgaaagc aacaggtata gttagaagaa tagatgatct tggaagggta
    58081 gttatcccaa aagagataag aaagacatta agaattagag aaggagaccc tttagagata
    58141 ttcacagcaa aagatggaga agtcatactt aaaaaatact ctccaatagg tgaattaaat
    58201 gaattttcgc aagaatatgc tgaaacatta gctgaagtac taggatatgg agtgttgata
    58261 acagacttag attctataat agctgtgtct aagcttccta aaaaagatta taaagaaaaa
    58321 agtataagtg atgagttaga agcaataata agtgaaagaa cagtaaaata ttcaaaagat
    58381 aaaaacattt taccattatt caaggaagac caaaaagaat atactagtca agttatcatg
    58441 cctataatta gtgcatctgg tgactgtata ggttcaattg tagttgtttc aaaggataaa
    58501 gatacaattg cagaaagtga agaaaaatct ttaaaaattg ctgctaactt tttaggaaaa
    58561 caagttcaat aattttatca taaagacctt ttacatgaag gtcttttgaa gtttttgtgt
    58621 aaaatattga atatagcatt aaaaatatgg tatattaaag tttgttatat aaacagtatg
    58681 gaggttatgt tatgaacaat aacaaaaata aagactcctt tctaaaagga gcccttatac
    58741 ttgggctagc aggtgtaatc gtaaaaataa tgggtggatt ttttaggatt ccccttggaa
    58801 acatgattgg ttcagaagga atgggatact atcaggctgc ttatcctgtg tatactctat
    58861 ttttaacatt agctactgct ggatttccaa cagctgtggc aaaattggtt tcggaaaaag
    58921 ttgcaattgg aaattttaaa ggtgcaaata agatatttaa ggtatcacat acagttttat
    58981 tcattaccgg tataatatca ttttgtatat tattttttgg agccgactac atagtaacaa
    59041 acgtaatgaa aaatccaggt gcgttatact ctatgaaagc cattgctcca gcattactat
    59101 ttgttcctgc aatgtctgca tatagaggat attttcaagg gagacaagat atgactaaga
    59161 ttgctgtgtc tcaagttgca gagcaatttt ttagagttgt attaggtctt actttggctt
    59221 acttgcttat gaagagtcta ggtcagactt ttggtgcagc aggtgctata tcaggggcta
    59281 caataggttc aatagcatct atgctttatt tggtgtttgc ttatatgcta gggagaaaag
    59341 agcgtagagc agagatagat gcaagccaaa gatttaaaga tgaaagagtg tcttatattt
    59401 ttaaaaagct tttaactgtt gccattccta taacaatagg agcatcagta atgccacttg
    59461 taaatatgat agataatgtt attgtaataa gaagacttat ggaagcaggc tttacataca
    59521 aagtggcaaa ttccatgttt ggtcaattaa caggtatggc aatggccaca ataaatttac
    59581 cagcagtcat aacaactgca atgagtatga gcttagttcc agcaatttct aaagcatatg
    59641 cattaggaaa taagtcaaaa gctagaaaag atactaagag tgcagttaag gtaactttac
    59701 ttatagtatt gccttgtgcg tttggtatgg cttctttagc aataccaata atgggtcttt
    59761 tatttccaca tgaaccatct tctgtaggaa ctattttgtt tactcttact ccatgtgtat
    59821 tatttttagg attaatacag acattgactg gtataattca gggaatggga aaaccaatag
    59881 tacctgtaat tgctttatgt gttggtatgc tatgtaaaat tgtaataagt tatactttaa
    59941 caggtatacc agatattaat gtactgggtt cagcatttgg tacagttaca gcatactttg
    60001 tggcagccat gattaactta ctatatgtta aaaaacacat gaatgtaaac ttttctaaaa
    60061 aagaatttat aataaagcca tttataacag ttatgacaat gtttataatg gttaagttga
    60121 gttatggtgc tttagttgga ttcttaggaa acagcatatc tacaataatt gcaatatgtg
    60181 ttggtggaat tgtatatgta gtagtaatac ttggaatagg tggaattaaa aaagaagaga
    60241 ttctaactat gccaaaaggt gataaattat ataaattgtt aaaaaaagtt aaacttataa
    60301 gataatatag gaatatatat gcaaataaag gaaatactat acaaatagta aatctcctta
    60361 tttgtataat aatataaaaa taaagtgaaa tttgctaata ggagtgatgt tatgggtaag
    60421 ataagtattg taggtcttgg accaggagac tattctctta taagccaagg tgtgttagaa
    60481 tctttaataa atagccctaa agtttttttt agaacagaga aacatccaat aattgataaa
    60541 cttaaagaaa ctatacaata cacttcttta gactactttt atgaaaaaga ggaaaatttt
    60601 gataatgttt atttgaaaat atctgaattt atcatagaag aatcagcaaa gggtgaccta
    60661 gtgtatgctg ttcctggtca tccaagagtt gcagagaaga ctgtaagtat aatagagaga
    60721 ttagccaatg aaaagcacat agcagtagaa atattgcctt ctatgagctt tgtagacgct
    60781 atgtttaact atttggctat agacccatct gagggattta aactggtaga tgcttttgaa
    60841 atagaaaatt catatataga cttggataca agtatgataa taactcaggt atatgataaa
    60901 tttatagcat ctaatgtaaa gttaaaactt atggaacatt atgactataa tcaagaagta
    60961 tgtatagtaa atggagctgg tgtcaaagat ttagaaagta aaaagtttat aaagctttgt
    61021 gaattagaca gaaatgaaaa cttttttgac tatttaacta gcttatatat acccaaaagt
    61081 tctaaaaaga tgtataatac agtacatgac ttagaaataa tagttaatac acttagaagt
    61141 ccatctggat gtgaatggga taaaaaacaa acacatcaat cattaaaaaa ttctgttata
    61201 gaggaagcat atgaactttg taatgcaatt gataataatg atatagatga aatggtagaa
    61261 gagttaggtg atgttctttt acaggttata ttccattgtc agataggaaa tgaggagggc
    61321 tactttgatt taaaagaagt agtaaatggc atatgtaaaa aattaattca tagacatcct
    61381 catatattta gtggtgcaaa attagatatg tctaagtttg agaaaacttg ggaagaatta
    61441 aagaaagaag agaaaggcga aagtactata acagatggcc ttagaaggat accaaaacat
    61501 ttaccagctc ttatgaaggc tagtaaagtt caacataaag cagcacttgt cggctttgat
    61561 tgggacaatg tagaagatgt atttaaaaaa attgaagaag aatatcagga attacttgac
    61621 gaacacaggg tcgggaatat aaaatacata aaggaagagc taggcgattt attattttcg
    61681 atagtaaatc tagctagatt tttagacata gactcaggag aagccttgaa ctgtactaca
    61741 gaaaaattca taaatagatt tgactttata gaaaaaagtg caatgaactt gaataaaaaa
    61801 ttagaagaca tgacacttga agagatggat gagttttgga atcaggctaa aaaacaataa
    61861 aattgtggaa ggaaatttgt caaaaatcta gaacttttat cacaaagata gaagtctata
    61921 ttataaacat ttttaggagg taaatatcgt gaataaagct gaattagtat caaagatggc
    61981 agaaaaaagt ggattaacaa agaaggaagc agaagctgcg ttaaacgcat ttatgagttc
    62041 tgttcaagat gcactagtaa ataatgaaaa agttcaatta gttggatttg gaacatttga
    62101 gacaagagaa agagctgcta gacaaggaag aaatccaaga gatccagagc aagttataga
    62161 tataccagct tctaaagcac cagttttcaa agctggaaaa ggattaaagg atataataaa
    62221 tggataatta gaaatcttgt gaaaaagtgt ggataaattt gccacacttt ttctatagaa
    62281 ccaaggaagg tgataattat gagattagat aaatttttaa aagtatctag gattataaaa
    62341 agaagaactg ttgctaaaga tgcatgtgat aaagggattg tcactataaa tggaaaagtt
    62401 gctaaatctt cttcagaggt gaatattgga gatacattag aaatacaatt tggagagaag
    62461 aagatgaagt ttaagataaa tgaagtaaaa gagcatgtat taaaaaatga tgcaaaagaa
    62521 atgtacaaaa ttttagaata aaattaatag tcctctagta tattaatagt agtattattg
    62581 aaatatttac tggggggata ttatggaaca gaacataact ttgaaagata gatcaaagct
    62641 ggttatttcg ggagtcgagc acatatactc ttttaatgac aaaagagtag agctcaaaac
    62701 ttctgtaggc gagatggtaa ttgaaggcga aaacctagat atgagcaagc taagtataga
    62761 cgaaaatata attagcatag atggaactat aaattctatg gtatatgcaa agcctaaaaa
    62821 gccacaagag agtttcttga aaaaggtatt tagatagatg atacctttta ctcaagatgt
    62881 aagcattttt tatgctacta tttatggtgg aatactcata ggggtgttgt ttgattttta
    62941 tagagggcta agagggaatt ttaaattcat caattatttt gctataatct ttgatgtatt
    63001 gttttggttt ctagctacgg ttataatatt cgtaacaata aaccttacag aattttttga
    63061 tttaagatat tatcattttg tggctttgtt tattggtttt attttatact ataacaccat
    63121 aagtaaaatt gttttatcaa ttatcaataa aataattcgt tttgtgagaa attcttttaa
    63181 aaaagttaca cattacatag tcagtttttt aaataatttg tattacgtta taatctattc
    63241 actacaccta ctatttgata ttatatttta tataccaaac atatttatag caacaagaaa
    63301 aagtataaaa agaaaatcaa ataaaaaatt aaagaacaag aaaaaatcaa aacctaagaa
    63361 gaaaaaaaat aaaactaaaa aaagggtgta ggaaaaatga acttaagaaa aaaattttca
    63421 ggacagatta ttgttatcag tttattttta ggaatatcaa tttttagtat gatgacagga
    63481 tttgtatttg agtatacaaa agctaaggaa tataaaaaag aaatagcaag tctaaataaa
    63541 caattaaaaa agactgaaat acaaataaac agcttaaaga aagatgaaaa atcatatgaa
    63601 ggagacttag aggatatagc aagaaaaaga ttgaatatgg taaaaccaaa tgaaacagtt
    63661 tatgtagaca taaatcgttg attaaaactt gctaataagt ttacagatgt attgatttta
    63721 aaatttaaga tttaataaaa aagtcattca gttaaggatg gctttttgtt tttattgagg
    63781 agggaatttc ataatgaaag taggagctat agatattggg acaaactcaa tgagattatt
    63841 gatagcagat tatagagatg ggaattttat aaacagaaaa aaatttgtta atacaactag
    63901 aattggttat ggtgtggata gtaaaggata tataaaagaa gatgctatca aaagaaatat
    63961 aaaagcatta aaagaatttt ctgataagtg taatgatgaa aagtgtgaaa aaatttattg
    64021 tatggggaca tctgctctta gagacagtaa aaatggagat tactttgtta aattagctaa
    64081 agaagaaact ggaatagatg ttgatatagt tacgggaata gaagaatcaa atttgggctt
    64141 taagggtgtt ttagaaggaa tacaaggtga tgatactatt attgttatag atattggagg
    64201 aggttcaaca gaatttattg taggtgattc agaaggaatt aaatttaata aaagtgagaa
    64261 tgtaggtgct cttaggatga cagagaggtt tttgaagcac gatattattg aagatgaaga
    64321 gtttgaagct atgagttcat ttatatatga tgagattgac aaaacgttaa atcatataaa
    64381 agacagagga ttaaaaactc ttataggtat aggaggtacg attacatcat tatcagcaat
    64441 gaatcaagaa ttagagattt actctatgga aaaaatacat aatagtaaaa tacacaaaaa
    64501 agaaatagaa gaaattctac aaaaattaat aaagatgaca ttaagtgata aaaagaactt
    64561 aaaagggcta caagaaaaac gtgcggatat tataactgct ggtgtaaaaa tattgaatat
    64621 aataatggaa aaattagaaa tagaagaaat aaccgtaagt gaatacgata atttggaagg
    64681 cttaatatgt caaaagtcaa aaaaaatgta ttaatatttt ttgattttta aaaccgatta
    64741 tgacaaaaaa aattactcct ctttgttaca attcttaata taaacaaaag aggaggtttt
    64801 tttatgcaaa gaagtgtagc agttgtgaag aataagactg taaaaccaaa tgtcataaaa
    64861 ttaataagcg gatatattga ttttactacg gcggcattcg ctgtgatggg atttttactt
    64921 agtaggtcta ttatagtaga ctctgtagct cctttaggaa ttgccttttt tatatgtgca
    64981 tcaaagattg ataagtacaa gatacctgta ttcatatcaa cattattagg aatattgtta
    65041 tcatctaatg attttggaaa tgtagcaaaa tatgctgtgt gccttacaat atttatggct
    65101 atgaataaaa aattaaaatt atcagattca attgctaagg ttgcatttat aggtgcaata
    65161 atactacttc caatctccat gggacaagct ttcttttcaa ataggtatgt atatggtctt
    65221 cttatagcag gagtagaagc aataataatg tttatatcta tatatgtgtt ttcttatggg
    65281 ataggtttaa tggccaatgt caacaatagg atgtctgtac ggacagaaga agccatatca
    65341 ataagtttac tgctaacctt tagcataatg ggaataggag atatatctct gtttggaata
    65401 tcaataagga ccattttatc gaccgtattg atattgctga cctcaatagt aggtggagct
    65461 accatgggag catctagtgg tgtaattatt ggtgtagcat ccattttaaa taatatgaca
    65521 tcagctgttt atatggggat atattctttt tcaggattaa taagtggagc atttaataaa
    65581 ataaataaat atttctgtat actaggatat attctaagtt ggacaataat atacctatat
    65641 acttctggta ttacttctaa tatgatgcaa ttaagagata tactattagg ttgtttgata
    65701 gtactagtat tgccagagag attatttaat aagattgaaa aattaataaa aagtaatgtg
    65761 gcttcaaatg aaatcgtata cgattatatt atgagaagca aaaacctaac taatagcaga
    65821 ttgaacagta tttataaaac ttatgatgac cttgcagata catttgataa gataagagaa
    65881 aaagacaaag tgctagacca aagagacata gctaatgtaa tagatatgat tcataatgat
    65941 gaatgtaaaa gttgtagtat gagaagaatg tgctgggagt caagatttaa tcatacatac
    66001 actatgatat ataacattct tgaaaaaatt gaggagaaag gggagttaag tctaaacgat
    66061 atacctaaaa attttagaaa ggaatgtatg aaaccagagt caatagtgaa aatatcaaat
    66121 cactattaca aaatgtttgt attagattat gattggagtg tgaagttttc agaaagtaga
    66181 aagttaatag caaatcagat aagaagtatt tcaaaatcca ttaaatcctt atctcaagat
    66241 ttagaaggcg acataatgct agatatagaa aaggaaaaaa atatatatga acagttagaa
    66301 agatatgata taacagttga taaagtaagt tacttaacaa aaagtaacag tgagtttgaa
    66361 atatcaatag aaaagaaaac ttgccatgat ggatgtatgt gtgaagataa aattgtaaat
    66421 ataatttcag acctagtagg ggaaaatatg tctgttagaa aaataggatg tcactgttta
    66481 ggaggtaaat gcaaggcaac attcgttaaa tctcaaaaat ataaggctgt tacagaagtt
    66541 tcagcaatgt caagggatgg gcatatcctt tgtggagaca attacacata catggagata
    66601 aatgatggta aatacatgat ggctataagt gatggtatgg gaaagggtaa aaaagcatat
    66661 gaagaatctt ctgccactat tgatatactg gaaaaaatga tagatgcaaa aattaaagat
    66721 gagatagtca ttgatactat aaacaatatg ttgttgttaa aatcttcaga agagatgttt
    66781 tcaactctgg atttgggaat tttagattta aaacgaggtt gtttagaaac tataaagatg
    66841 ggagcgtgtt ctacttatat aaaaagagag gatggagaag ttgacttaat atcatcatca
    66901 tcattacctg tgggtatatt atcagatgta aaaattgata gaaaaaatgt aaaagttaag
    66961 gaaggagact atgttataat ggtttctgat ggaattgtag atgcaggaag aaataacaat
    67021 ttaggagaca attggttaat atacttctta aagaatatag agactacaaa tccaaaggaa
    67081 atttcaaatt taatattgga tagagcatta gagttacaag cgttacaaat cgaagatgac
    67141 atgactgttt tagttacgaa aatatgtaca aaataaggtt tacaagatta tgtgtataag
    67201 atgtggataa cttgtgtata atatgttaat agacttttga aaaaagtata gttattaaca
    67261 aaaataacat ataaatcaac aaaatttgtt gataagtatg gcagaaatgt taataaaatg
    67321 taaaaaaaga aaaaacaaaa gctattatca aaagagattc atgttgaaat aactaaattt
    67381 ctaagtaaag aaaacctgtg aatatgttga ttattatgtg gataactatt aattcgatat
    67441 ttatttatat agtatgaata attgaataat atatctaaaa aaagccatcc tattattaag
    67501 taaacttatt gaaaggatgg ctttttatga atagaaaatt gtgcaaagca ataatacctt
    67561 tatttatttc tattttttgc atgttaataa taagtataaa agctgaccaa ggatatcaaa
    67621 agaataatga cagagcaatt actgtcatga ataataatga tactgaagat gatgctgaaa
    67681 aaaaacaaga aatagactat gaaaaaacat gttggaattt gcaatctttg tttaaaagtg
    67741 atgaccaatg gaaaaaagaa cttaagagtt ttgataaaga tactaaagag ttgaaaaatt
    67801 acataggtaa agtaacaaaa tcgccaacac atttgtcttt tgccttgaac attaaagaaa
    67861 aacttgatat aagactaaat aaactttctg cctatgtaaa attaaaacaa gatactaata
    67921 aaaattcgta taagtactta gatatgaatg atagtatgag taaatcctat gggaattata
    67981 tggggatttg ttcagattta gagttagaaa tactaaaatt atcaaacaaa gattacaaga
    68041 aaattatttc aaataaaaat ataaatagga aatatggtgc atatttaaga gatataagaa
    68101 ggaataaagt acattatttg gatgataaat cagaagatat tctaagcaaa gtatcttcaa
    68161 taagtagtct tccaagtcaa gtgtatgaat tatttagaaa tatggataga aaaactaatt
    68221 taacaccagc tcaatatgcg agtgaattag agtcatcaga tagagaaaat agaaaaaaag
    68281 cttatcaaga tgaacttata acttacaatg ataatataaa tacaattgca ggtttaatta
    68341 ctggtcaagt taaaaagaat attttttatt ctcaggaaag aggttatgag tcttctttag
    68401 aaatgtacct ggaagctgat aatgtagatg aaaaagtata caataatttg ataaggactg
    68461 taaatagtaa tatggacagt ctacacaaat atatagattt aagaaaaaaa gtattaaaat
    68521 tagataaggt tcattcatat gatatgtttg tgcctatggt taaggctgtt gataataata
    68581 ttaattatga aaaggcacaa agtatagtat attcagcgtt gtcaccttta ggaaaagaat
    68641 acaatgatgt agtatataaa gctttcaatg aaaaatggat agatgtttat tcacatgaaa
    68701 ataaagtaag tggtggatac tgcttatctg tttatgaaaa tcatccatat atacttttaa
    68761 attataataa ttcattaggt tcagtttcga ctttatcaca tgagcttgga catgctgtat
    68821 atgaatactt aagttctaag aatcagaatt attttaattc aaatccgtct atatttactc
    68881 acgaagttgc atctacaacg aatgaagcat tactttacga gatgcttata aaagaagcta
    68941 aaggcaatga tgaaaaagct tattatataa cacagtattt agacttgata aaagatactc
    69001 tttatacaca aactatgtat gctgaatttg aaaaaactat acacgagtta gtagaaaaag
    69061 gaaaaagtgt aaacgcatta gtattaaatg atgtttgggg acaacttctt aaaaaatatt
    69121 atggacaaag ctatgaatta gaccaattat ctaaggtcgg atgggctaga atcccacatt
    69181 tttataatag tttctatgtc tataaatatg ctacaggttg tagtgcagga gtaagttttg
    69241 cccaagacat cctaaagaat ggttcagata actatatgag ctttcttaaa aagggaagtt
    69301 cagattatcc aataaacttg ttaaaatcaa gtggcataaa tttaactaat acaaaaccca
    69361 tagaaaatac aatcaaaaaa tttgatagtt tagtagcaga acttgaaaag ctattataaa
    69421 cattattgta atccacatgg taaatatatc gaattaatga taaaatatac taaaagttta
    69481 aagtcctatc aagataggac tttttattta ttaattagtg ggagatgatt ctatgcaagt
    69541 taaagttaaa aaggctgtaa taccagcagc aggtttagga actagatttt tgccagctac
    69601 aaaggcacaa ccaaaagaga tgttaccaat agttgataag ccaactctac aatacataat
    69661 agaagaagct gtggcctcag gaatagagga aatactaata ataacaggta gaaataaaaa
    69721 atctattgag gaccactttg ataaatctgt agaattggaa cttgatttag agaaaaaagg
    69781 taagaaagaa ttgttggagg tagtacaaaa catatctaac atgattaaca tacattatat
    69841 aagacaaaaa gagccaaaag ggttgggaga tgcaatttat tgtgcgaggc attttatagg
    69901 tgatgagccg tttgcagtaa tgttagggga tgatattgta gataatgatg taccttgtct
    69961 aaaacaacta acagatgcat atgaggaata tagaactaca atattgggtg ttcaaaaggt
    70021 aaatcaagaa gatactaata aatatgggat aatagaagca aaaaatatag aaggtagagt
    70081 ttacaaggta aaagacatgg tagaaaagcc agagtcagga aaagctcctt caaatattgc
    70141 tattttaggt agatatataa taactcctga aatctttgat attcttaagg atttaccacc
    70201 aggtaaaggt ggagaggtac aacttacaga tgctttaaaa atattatcta agaaagaagc
    70261 tatgtatgca tacaattttg aaggcaagag atatgatgtt ggggacaagc taggattttt
    70321 agaagctact gttgactttg ctctaaagaa agaagacctt aaggaagatt ttataaaata
    70381 tttgaaacat gtatgtagtg aatttgataa aaataacaat gtgttataca ataatacaga
    70441 tgaaaaaata gatttaattg aagtcaatga agctagagaa cttgaaattc aaaaataatt
    70501 aatgaaaggt gatgtatggt ggcaaaatca agtcagaaaa gaattaatag aataatagct
    70561 gttgggattg tgggaatcct cgtaataacg agtataacag ctgttttgag tatgattgca
    70621 actatgtaat aagataaatg acagcagttg atatacataa atataaaaac catatataac
    70681 ttgatgtagt caaaaactta caatatttta gttatatatg gtttttagaa tttataaatg
    70741 taaaaaaaaa tttttataat agttgcatat tttaaaggaa aatattatta taagatataa
    70801 atatagtgat aaatttataa aaacgtgcta agattggact tgaaatacag taatatagta
    70861 ggtaataatg ttttaaattt tatttattga ccataatata taaataatga tattattaga
    70921 cttaactgtt taaataaaac ttttcttttt catgccttca atttccaaaa taagtatgta
    70981 tataaatgaa tatatagatg aggaggattt ttttgatttt agatgataaa gtattagaag
    71041 gattaggtag taagacctta gttgagctac gagagatagc taaagaatta aaaataaaat
    71101 caattactac atataagaaa aatgagttaa ttgagattat aaattccaaa agtaaaaatg
    71161 aggaaaaagt aaacgaagag actaaagaaa aaaatgaaga tattaaagaa gacataaaga
    71221 atagaaaaga aaatatagag cgtgaaagta tagaaaaaga agttgaatat aagagcccta
    71281 ccaaatcata caacaagaaa gaaatagata cccaaaatat ttcccaaaac caagaacata
    71341 gaaatcaaag aaatgactat aataagacaa cgagtgtaaa tgatagtaat aagtcatcaa
    71401 atatatctaa tagaatggtc agaaataata ataaaaacta ttatatgcct aaacaagttg
    71461 atgaatctaa gatagtagat gaatttaaca catcaaaaga ggatgaagta gtaggtgtac
    71521 ttgaaattct gccagatgga tttggttttt taagaggttc aaattatttg tctactgaag
    71581 gagatgttta cgtttcacca tctcaaataa gaagatttaa tatgaaaaca ggagacaaga
    71641 ttaagggaat aactagacat ccaaaaagtg gagagaaatt tagagcactt ttatatgttc
    71701 aaaaaataaa tgatgaaaac cctgacacag ctatacaaag aaatgcgttt gagacattaa
    71761 caccaatatt tccagaagaa cgacttacac tggaaactaa tagaaatgaa atagctacaa
    71821 gaattataga tttaatttca cctataggaa aaggacaaag aggattgata gtagcacctc
    71881 caaaagctgg gaaaacagtt cttttaaaaa gcgttgcaaa tagcatagct aaaaatcatc
    71941 caaatgttga acttatagta ctccttatag atgaaagacc agaggaagtt actgatatga
    72001 aagaatctat tgaaggagac gttatatatt ctacttttga ccaagtatca agtcatcatg
    72061 tcaaggttgc agagatggtg ttaaatagag ctcaaagatt agttgaacat ggtaaagatg
    72121 tagtaatttt actagatagt ataactagat tggcaagggc atataatctt acaatatctc
    72181 ctacaggtag aactttatct ggaggtattg accctggtgc acttcatgga cctaaaaagt
    72241 tctttggtgc agcaaggaat attagacagg gtggttcttt gacaatatta ggaacagcat
    72301 tggttgaaac tggttctagg atggatgatg taatatttga ggaatttaaa ggtacaggaa
    72361 atatggagct acatttagat agaaaattag cagaaaaaag aatattccct gcaatagata
    72421 tttacaagtc tggtacaaga agagatgatt tattgcttga tgatgaagaa aagactgctc
    72481 tttggagatt aagaagagaa atgagtaata attcagtaat ggaaattaca gataaagtca
    72541 ttgaacttat aaagagaact aaggacaata aagagtttgt aaaatcaata aagaatttgt
    72601 aaaatcaata atacaaatgc aaatatggat tatataaact aatagaaaaa tcacttgaat
    72661 ggcataataa gttgtgctat aataatgtag ttgataatgt aaaaagaaag ttaatataat
    72721 taattttttt aaataaagag aagaggtgac tataatgcaa aaggaaatac aaccaaaata
    72781 caatccagtt gaagtgcgtt gtgcttgtgg gaacacattt gttgctggtt caactaagga
    72841 cgaaataaaa gttgaaatat gttcagagtg ccatccattc tatacaggaa aacaaaagaa
    72901 catagaaaaa ggtggaagaa tcgacaaatt caagaaaaga ttcaaaatgg actaattttt
    72961 ataaaagtgg agttggagga agccaactcc tattttatta caaaaaaaca ttcatattct
    73021 actttaaatt gtctttttgt attataataa taaatatgta aaattgagta ttctaaatac
    73081 aataaaatga ggtgagtgga tggcaaaaca agctgtaggt gggcaagctg ttattgaagg
    73141 tgtaatgatg cagtcaaaag acaaaagagc ggtagctgtt agaaaaagtg acggagaaat
    73201 agcactaaaa gaagatagaa taaaaagttg ggtaagagat aagaatatag ataagatacc
    73261 ttttgttaga ggttcttttg ttatgataga tacaatgatt caaggcataa aaagcttaaa
    73321 cttttcttca gagtttttta tggaagaagc tgaagaggat aaatttgatt tatttataaa
    73381 gaaaattttt aaagataagg caaatgatat aattataata ttttcattgg tcatagcaat
    73441 gttactttct gctggattat tcatttttat acctacattg gtaggaggag ctttttctaa
    73501 agtaatgcct aatgatttta tgcttaattt aatagaagga atcataagga tagctatatt
    73561 atttgcatat atagtattga tttcacgaag taaggatata gaaagagtct ttcaatatca
    73621 tggagcagaa cataaatcta tatattgcta tgaaaatgat ttagaactta cagtagagaa
    73681 tgctagaaaa tttaaaagat tacatcctag atgtggtaca aatttcctat ttatagttat
    73741 ggctgtatca ataatattgt ttgcattttt tggttggcca aatccaattc ttagaatatt
    73801 tatgagaatt atatgtgtgc ctatagtagc aggtttatct tatgaggtaa ttagagttct
    73861 tgggaaatat gataatggat ttactaaaat tattgcatat ccaggaatga tgttgcaata
    73921 ttttacaaca aaagagcctg atgatgagca attagaagtt gctttggaag cattgaaagc
    73981 agtagtagat tagagaaaag gatatttgtt atgacgataa aagatataat tattaaatat
    74041 tcagataaac taaaagatat aagtgatact ccaagattag atacagagtt attgttacaa
    74101 aaaacattgg gtgttgatag attatatatt cacttaaatc tgaataaaga gctaactgaa
    74161 gaacaaaaaa caaaatttat gggttttgca gaagaaagat taaatggaag accaatagca
    74221 tatatagttg aaaataggga gtttatggga ttagattttt ttgttaagga aggcgtattg
    74281 attccaagac cagatacaga aactttagta gaagaaataa ttgaaatatg tagagaaaaa
    74341 aaagatgtaa gtatattaga tataggaact ggttctggag ctattacaat aagtttagca
    74401 aagtacattg aaaattctaa aatcatgtct tttgatatat cagagacagc attagaaata
    74461 gctaaaaaaa atgctattat aaatgaagtt ggtgaaaaaa taaagtatat taattctgat
    74521 ttatttacag caataagtga tagtaacata aaattcgata tcatagtttc aaatccacca
    74581 tatattaaaa aacaagatat agaaactctg cacaaacaag ttaaagatta tgaaccatat
    74641 aatgctcttg aaggtggaga agatggatta gacttttata gaagaataac agaacaaggt
    74701 aagaaatatt taaataagtg tggaatatta gcatatgaag ttggtcataa tcaagctgaa
    74761 gatgtaataa atattatgaa aagtaatgga tataaaaaaa tatacacaaa gaaggatatt
    74821 caaggcattg atagagttgt tataggttac aatatatgat ataatcaaca attgatatgt
    74881 tacatttaat cgaggtgaaa gtatgttaaa aaaattagag gtattggaag atacatataa
    74941 agacttaagt gaaaaaatag gtgatccgga tgtaataaat gatcaaaaag tttggcagaa
    75001 atatataaaa gaacatgctg acttggaacc tattgttatg aaatacagag agtataaaag
    75061 cgttttagat agtataaaag aatcaaaaga aattttacaa gaagagtcag atgaagaatt
    75121 aagagaatta gctaaaatgg agttagctga gatggaagaa aaagtagcac ctctggagga
    75181 agaaataaaa atactattat tgcctaaaga ccctaatgat gataagaacg ttatagttga
    75241 aattagaggt ggagctggag gagatgaagc agctctattt gctggtgatt tatttagaat
    75301 gtactcaagg tatgctgaaa gaagaagatg gaaaatagaa ttattgagtg caagtgatac
    75361 tggagttggt ggatataaag aagtatcttt tatgataaaa ggtaaaggtg cttattcaag
    75421 attaaagtat gaatctggag ttcatagagt tcaaagaata ccatcaactg aatctggtgg
    75481 aagaatacac acatcaactt ctactgttgc agtattacca gaagttgaag atgtagaggt
    75541 tgaaataaat ccaaatgatt taagaataga tgttttccgt tcttcaggta atggaggaca
    75601 aagtgtcaat actactgact ctgctgttag ggtaacacat atacctacag gtgaagtagt
    75661 atcttgtcaa gatggaaaat cacaattaaa aaataaagag caagctttaa aaatattaaa
    75721 agctagactt tacgataaag cattggcaga acaacacaaa gatatagcag cagaaagaaa
    75781 aagccaagtc ggaacaggag acaggtcaga aagaataaga acatataatt tccctcaagg
    75841 tagaataagt gaccatagaa taaatttaac tttatataag ttagatgcat tcttagatgg
    75901 agatatagat gaaatgatag atgctttaat tactgttgac caaacagaaa aaatgactgc
    75961 aatataagta atgttttaaa gaagctgttt cttcctagaa attattttct attttgaaac
    76021 agctattttg cattttaggt catgttatca tattctattg tatataattg ttatatacaa
    76081 gtataggagg gttatatgat actaaaagta acaataattg ggcttttggc aggtgttata
    76141 gggacaggat taggaggagt aatatcagcc atttttaaaa gagaggtcga taaatatcta
    76201 agttttttta tgggattatc tggtggaata atgttggctg tagtggtgtt tgatttaatg
    76261 aaagaatcca tggacaaaat ggggataatc aatacagtta tatttacgtt tgttggagct
    76321 ctcataacta tgtacataaa aacaaaatta gatgtgtctg gaaatatggc atctggatat
    76381 cttatattca taagtatatt actacataat ttaccagaag gactagccat tggttcttct
    76441 tttatgtcaa ctgaaagttt aggtattaca cttgctatag ttataggtct acataatata
    76501 ccagaagggt tagctatggc attaggctta gtttgcaata aaatgaaact aagtaaagtt
    76561 attttgttta ctgtaatagc tggattacct atgggtctag gaagtttttt aggtgtttat
    76621 tttggaggag tatttacttc tcttatagga gtatttcttg ctacagcagg aggtactatg
    76681 atgtatgttg tattggaaga aatttttcca cattcaaaaa gtgtgtattg tataattggt
    76741 ttcttgttag gtacaatgat tgtaaattat atataaaatt aaaatgaaag caggcataca
    76801 aaagatgaaa actattataa gtaatattga tataaataat attgattatg aggaaataaa
    76861 aatacaagca aaacttctta gagaaggtaa aacagttatt tttccaactg agacagtata
    76921 tgggttgggt gcaaatgcat tagatgaaaa tgcagtaaag aagatttatg aagctaaagg
    76981 aagacctagt gacaatccat tgatagtcca tatatatgaa aaagaagaag tatatgactt
    77041 agccaaagat ataagtgata aagctaaact tgttatagaa aagctttggc caggacctat
    77101 aacaattata ttaaataaaa aagatataat tccatacaaa actagtggag ggcttgatac
    77161 agttgcaata aggatgcctt ctaatgttat tgccagggca ataataaaag aagctggtat
    77221 acctattgca gctccttctg caaatatctc aggtcgccca tctccaacaa aagcaaaaca
    77281 tgtatatgaa gagatgaatg gaagagtgga tggaatagtt ttaggaggag acagtaattt
    77341 tggattggag tcaactgtat tggatttaac agaagagact cctatgatac tgagaccagg
    77401 aagtataact aaagaagttt tagaggaact tcttggagaa gtaaagcttg acccatcatt
    77461 aagtaaaaaa gaagataatc aaaaagctaa agctcctgga atgaaatata aacattattc
    77521 tccaaatgca gatgtatata taatatctgg aaaaagagaa aatgtagcaa caaagattaa
    77581 tgatatgata aaatctaata gagacaaagg tctaaagaca ggtgtaatgt gtattagtag
    77641 aaaccgagag ttttatgatg gagaagtaat cgatttagga aactctcttg aagaagttgc
    77701 aagtaatctg tttgatgctc ttatagaaat ggacaaaaag ggtgttgatg tcatatattc
    77761 agaagagttc cctaagactg gagttggaca agcaattatg aatagactac tgaagtcagc
    77821 aggatacaaa gtgataaagg cttaagaggg agttgtaaaa tatgaatata cttattgtgt
    77881 gtacaggtaa tacttgtaga agtcctatgg ctgaggctat actaaggaaa gcaattaaag
    77941 aatctggaag aagtattgaa gaatattcta tatcatcagc aggtatatct acagcaaatg
    78001 gaatgggtgc ttctgaaaat tctatagaag tactaaaaga aatcggtata gacttatcaa
    78061 atcatagaag taaagtaatt actaaaaaat taattgatga atcagatata atactaacaa
    78121 tgactaaatc tcataaagaa attttagtac aagcagttcc aaaatgcaaa gaaaaagtgt
    78181 atacttttaa agggtttgca aataaaaatg aggaagatat atctgaccca tttggtggaa
    78241 atttggatat ttatagaagc actatgagag aaataatgta ttctgtcaac gaaatagtaa
    78301 aaaaaatatg aaacggagga taaacaaatg aagataggat taggctgtga ccatggagga
    78361 tataatttaa aaaaagaaat aataagctat ttagaaagta aaggcataga atgtgtagat
    78421 tatggaacta ataatgctac agattctgtt gattatcctg tttatggaga aatagttgca
    78481 aattcagtta taaataaaga agtagattat ggaatattat gttgtggtac agggatagga
    78541 atatctttag ctgctaataa ggttcctggg ataagatgtg cagttgtatc agatgtattt
    78601 tctgcaaaaa tgtcaaaagc acataatgat gcaaatatgt tatcattagg cgaaagagtt
    78661 ttaggaaaag gtctagcact tgagatagtt gaagcatgga ttaatacgga ttttgaaggt
    78721 gatagacatg ctagaagagt aaacatgatt aaatcaatag aagaaaaaca taataaataa
    78781 taaaaattac agttttatca ggaggaatta tcaaaatgag caaagtggta gagacaaacc
    78841 atccattaat acaacataaa ttaactttaa tgagagataa aaatactggt tctaaagact
    78901 ttagagagct tttaactgaa attgcaatgt taatggggta tgaaataaca aaagatatac
    78961 cattaaaaga tgtagaaata gaaactccta ttcaaaaaac atcatctaaa gtagttgcag
    79021 gtaaaaaatt agcaataatt ccaattttaa gagcaggtct tggaatggta gatggattag
    79081 tgagcttaat gccagcagca aaagttggac atgtaggact atatagagac ccagaaacat
    79141 taaaaccagt tgaatattat tgtaagcttc ctcaagacat aggagaaaga gatataatag
    79201 tagttgaccc tatgcttgca acagggggtt ctgctgtagc agctatagat ttacttaagt
    79261 ctaaaggagc taaaagtata aaattagcta atttagtagc tgcaccagaa ggtatagcag
    79321 aagtgcaaaa atatcatgat gatgttgata tatatgttgc atcagttgac gaaagattaa
    79381 atgaacatgg atatataatt cctggtcttg gtgatgctgg agatagattg tttggaacta
    79441 aataaaaata aataactata aatatttatt agggactttc ttatatagtg tatataattt
    79501 tatgtaagaa ggttcttttt tatatatttt ttctcaaaat aggaattatt gaattgataa
    79561 atttctcgaa ttgagaataa aaagttttta ccaattaaaa aagattgctt attttacaag
    79621 gttttataga atagtagtct attttaatgt ggcatgttag ttgcttacga ttaagattat
    79681 aggtattaag tgtattagtt aaattgagaa ttgaatatta ataatattat tgggagtggt
    79741 aatacatgaa gaattgtata gtagtaagag gggctggaga tttagcaaca gcagttattc
    79801 ataaattaca caacagtggt tttaaagtag ttgcgttaga aatagaagag ccattagcta
    79861 taagaaggca aatttctttt tgtgaggctg tttatgaaaa aaaagtaact gttgaaggtg
    79921 tagaatgtat attttgcaat actgaaaaag aaatagaaga agttttatct caacataaag
    79981 tagccttgat tatagaccca aaaggagaaa aattatctaa attaaatcca atcgttttgg
    80041 ttgatggaat actcgctaag aaaaacttag gtacaactaa agatatggca ccacttacaa
    80101 tagctttagg tcctggattt tgtgcaggag aagatgtaga tgttgttata gagacaatga
    80161 gaggtcataa tctaggaaga ataataacta atggtagagc agaaaaaaat acaggagtac
    80221 caggagcaat aaaaggtttt tctaaagaga gagttatgca ttcacagtat gatggcataa
    80281 tggaaaatgt atgtaagata ggtgatatag ttgaaaaggg agacctactt tgctatatta
    80341 ataattatga aaaaaaacat gaagttagag caacaatatc tggagtcctt agaggtttat
    80401 taaaggatgg agcaaaggta aacaaaaatt taaaaatact agacatagac cctagaaaag
    80461 aagaagttaa gaatgctttt acaatatcag ataaagctag atgtatagct ggaggggttt
    80521 tagaagccat actaaacaat gggatttatc cccaaaatta atttatatta gataaagcta
    80581 ttaatatttt attaagatga tataataaat ttaatagatt aaagagagca tatgacgggt
    80641 atttaaaatt taaatggtaa aaatgctctt tttttattgg acattcaatt aacatgtgta
    80701 tataatataa aaataagtat ttatttaggt tgaaatctaa taaatatata ataccagtac
    80761 ataagcaaag aggagaaaag taaattatga gaccatcatg ggatgaatac ttcatggaaa
    80821 ttgcagaagt agtgaaaaag cgttcaactt gtattagaag acaagtagga gctgtaattg
    80881 taagagacaa acaaatatta acaactggat ataatggttc tccgagaaat cttgaacact
    80941 gtgaaaatat tggatgtaag aggcaggaat taaatattcc ctcaggagaa aggcatgaat
    81001 tatgcagagc attacatgct gaacaaaatg cgataataca agctgctcat aatggtatta
    81061 gtgtagatgg ggcaactttg tatgttacta caagaccttg tgttttgtgt gcaaaaatgt
    81121 gcataaatgc aggtatattg aaaattgttt atgaaggtga ttatccagat gatatgtcta
    81181 cagaattatt aaaagaagca ggaatagaac tcataaaatt ttagtggata aaatttatat
    81241 gacaataaat gtcaaattat gacactgaaa tacaatggta tcaaatgatt aaaagaaagt
    81301 atttgaaaaa tcatcagatt agtagtaaga tatattttat aattaaataa atttatgaga
    81361 tagattaatt acatgttatt tattttataa taatgaggga tactatttat gagtaaaaga
    81421 aatacacata cagaaattgc aaagttgttt agtttaataa gccaaatagg attgatgatt
    81481 gtaatatcaa tattaggatg tactttctta ggaaaatttt tagatagtaa attgaatact
    81541 actccagttc tgacaataat atttttattg ttgggtgttg gaggtgcttt tagtggtgtt
    81601 tataaaactc taatagtata tacacgaagg aagtgatata ttggatataa aattacttaa
    81661 ccaggtaagg tcagtaacaa aaggtatagt aatatttgat gtagttttaa ttgtgctaat
    81721 gttgataaca tcaacattaa gtaaaccagt attattagga cttatatttg gctctataat
    81781 agctatgtta aattttagat tattggcgat atcactggaa aaatcagtga catttcctgc
    81841 tgggaaggca caagcttatg catcagcaca atatgctgtt agattattta taatggcagt
    81901 tgttctaatt gcatcagcta cagtaccaca tttaaatata atcggaacag cattgggatt
    81961 attgagcaca aagtttgtca tattatcaaa aaactttata aataaattaa agaggaagga
    82021 ggcataagta tgaatcaagc gcattattat ctcataggaa actttaagat tagtgaaaca
    82081 atactagtaa gttggggaat aatggccttt ttagcgatag catcttattt tttgactaga
    82141 aacttaaaaa aagttccaac tagtaaagtt caaatattct tagaatttgc agtaggtggt
    82201 cttgctaaaa tggttacaga tgttatgggt aaaaatacag taaaaagaat gccatatatg
    82261 ataccataca taggaagctt gtttttattt ttcgcatgtt caaatttaag cccattactt
    82321 ggattaaggt caccaacaac cgacttagat acaactgctg catgggcaat gattacattt
    82381 tttatgattt attttgcagg agttaaattt aaggggttat catactttaa agaattggct
    82441 caaccacttg ccataatgtt accaatcaat attataggtg agttagctag accaatatca
    82501 ttaagtttcc gtccatttgg gaatatttta ggtggaacaa taataatggg tctgttttat
    82561 caacttttag gttttatttc tagtttaata ccaggattat caataccaat tggagaaata
    82621 ttaataccag tcccattaca cttgtacttt gacttatttg caggattgtt acaagcatat
    82681 atatttataa tgttaacaat ggtatttata tcaagtgcag gagagttaga ttaagaaatc
    82741 aacttatatt aagataaaaa taaaataatt taaaaaatta aagttttaag gaggaaataa
    82801 ttatggaaac agcaatagta gcggcagcat cagcaatagg agcagggata gcagttgcaa
    82861 caggtatagg tgcaggtata ggtcaaggta tagcagcagc taaagctgca gaagcagtag
    82921 gaaaccagcc agaagctaaa ggtgatataa catcaacttt attactagga gttgctatag
    82981 cagagtcttc agcaatatat ggtttggtta tatcaataat acttctattc gtaaatccat
    83041 tctttaaata cttaggaatg tagatttagt ccttaaatat tttgagatag gtttataggt
    83101 aaatcatctc aaacttataa tcatgagatg aactttatca tctcgtgatt ataagtagtt
    83161 atgatttatg gaaaggagga taattatgga aaaagcacta gtaggtataa cgtgggaatt
    83221 tgtgttccaa attgttaata cttttataat atttttactt ctgagaaagc ttttattcaa
    83281 gccagtatta aatataattg aatcaagaga aaatgatatt aaaagtgatt tggtagaagg
    83341 cgaaaaagca aaaaatgaag gtttagctct gaaaaaagaa tatgagtcaa aaatcaattt
    83401 tgccaaagat gaaggtcaag aaatcataaa acaagcaact ataagagcag agcagaaatc
    83461 agatgatata gtaaatactg caaagaaaga tgctcttgat ataaaagaaa aagcaaacaa
    83521 agatatagaa caagaaagac aaaaggttat aaatgaaatc aaaaatgata tatctaacat
    83581 agcactactt gcagcatcta aagttattga aaaagattta gataaatcta aacatgaaga
    83641 attgatagaa aactttataa aagaggtagg ggaagctaaa tgataaatgt aatagcaaat
    83701 agatatgccg aagccctatt tcaattagga gaagaagaaa attcaactga tgtcttattc
    83761 aaagaactag aaaaagtagt agatatgatg actaaagtta gtaaagattt ctacaaagtt
    83821 ttaaaatcac cattagtttc aaaatcagaa aagaaaaact tggtagaaat tatttttagt
    83881 aaagaagtaa gttctaacat aaaaaacttt ttaaaagttt tagtagataa agatagaata
    83941 tcatatttag aagatatcga attagcttat aaagaattat taaataagaa aaacaatgta
    84001 attgatggag tagcaataag tgctattcca atgagtgaaa cagatattaa agaacttgaa
    84061 gtcaaacttt caaataaata caacaaaaat gttactatag aaaatgttgt tgacaagact
    84121 atactagggg gagttctagt tagaattgga aatgaacaaa tagatggcac tgtgaaaact
    84181 cgtcttgata agatgaaaga aaagctatct gaagtaatat cttaagaatg gcggtgaacc
    84241 catgaactta aaacctgaag aaataagttc tataattaaa cagcaaataa aaaattatga
    84301 gaataaagtt gagttgacag atacaggtag tgttttaaca gttggggatg gtatagcgag
    84361 tgtatatgga ttagaaaaag ctatgtctgg tgagttgcta gagttcccag gtgaaatata
    84421 tggtatggca cttaacttgg aagaagaagt agttggagct gttatccttg gagatgacag
    84481 tgaaataaaa gaaggcgata tagttaagag aactggaaga atagttgaag ttccagttgg
    84541 agaagcttta ataggtagag ttgtaaactc actaggtcag ccaatagatg gaaaaggacc
    84601 tatagcgtac acaaaaacta gaccagttga atctgaggca cctggtataa tagatagaag
    84661 gtctgtttat gaaccattac aaacaggaat caaatcaata gattcaatga taccaattgg
    84721 tagaggtcaa agagagttaa taataggtga cagacaaact ggtaaaacat ctatagttat
    84781 tgacactata ctaaatcaaa aaggaaaaga tgttatctgt atatatgtag caattggaca
    84841 aaaacgttct actatagctc aattagttag ctctttagaa aaaggtggag ctttggatta
    84901 tacaatagta gtttcagcaa cagcatctga atcagcacca cttcaatata tagcaccata
    84961 tgctggagca gctatgggtg aagaatttat gtacaatggg aaacatgtac ttatagtgta
    85021 tgatgattta agtaaacaag ctgttgcata tagagaaatg tcattgttac ttagaagacc
    85081 accaggacgt gaggcatatc ctggagatgt attctattta cattcaagat tacttgaaag
    85141 agcagctaag ttatctgatg aattgggtgg aggttcaatg actgccttgc ctataataga
    85201 aactcaagct ggtgacgttt ctgcatatat accaacaaat gttatatcta taacagatgg
    85261 tcaaatatac ttacaaccag agttattcta ttcaggtgta agaccagcag ttgaccctgg
    85321 tatatcagta tcaagggttg gtggttctgc gcaaattaaa gcaatgaaaa aagttgcagg
    85381 aacattaaaa cttgcatatt cacaatatag agaacttgca gcattctcac agtttggttc
    85441 tgacttagat gaagatacta aaaagagact tgctcaaggt gaaagaatcg ttgagatatt
    85501 aaaacaaggt gagcatcaac caataaaagt tgaaaatcaa gttatgataa tatatgctgt
    85561 tataaataat catttagagg atattccgat agataatata gctagatttg aatcagaatt
    85621 gtatgcattt gtagataata attatccgga aatatcaaga aaaatattag gtggagaaga
    85681 cttcactcat gatttaacag atgcaataaa tgaatttaaa gaaaagtttg ttgttgaagt
    85741 ataatccttt ttaaaaaagg aggtaaatta attggcagga gctggaatca aagcaatcaa
    85801 aacacgtatg ggaactgttg aaagtacaaa gcaaataact aaagcaatgg aattagttgc
    85861 atcttctaag cttagaaaag caaaagaaaa ggctgaagat tcaagagaat attttaatac
    85921 tttatacaat acagtaaaag atattgccca aacaacacgt ggaattagaa atgaattttt
    85981 aaaagaaaga gaagtaaaaa ataaatgcta tgtagtagta gctggagaca gaggacttgc
    86041 aggtggatac aattctaatg tattaaagga atctgtctct catatgaaag gtaaaaagga
    86101 aacagtgatt actgttggaa agaaagcaca tgaatttttt tctaaaagaa actacaatat
    86161 agcaaaagat ataaatagtg ttgaagcttg tggatatgaa gatgcgacag aaatttcaca
    86221 aactattatt gacctgtata aaaaaggtga agtagatgaa gtttatattg cttatacaaa
    86281 atttaaatct gctttatcac aagaagttga aattttgaaa ttgcttccat tagtctttga
    86341 aaaagataca gacgaacaag gaatggcagt aaaagaagaa aaacaaaata gatcaaaagt
    86401 acaatatata ccatcagcag aagcagtttt aagttatatt gtacctcagt ttatatcagg
    86461 tacagtatat ggtggaattt tggaatcttt tgcatcagag caaggtgcta gaagaactgc
    86521 aatggaatca gccacagaca atgcgaatga aatgctatct aatttagagt taagttataa
    86581 cagagcaaga cagtcagctg taactcaaga aataacagag atagttggtg gagttgaagc
    86641 tctaaaatag ggaggttagg aaatggcaaa tgtaggtaaa gtagtacaga ttgtaggagc
    86701 tgtactagat gttaagtttg atagtgaaca aagcctacct aacttattaa atgcgttagt
    86761 aataaagtta ggagataaag aaatagttgc agaagttgct caacatatag gtgatgatac
    86821 tgtaagatgt atagctatga gtgcaacaga cggacttgta agaggtatgg aagttgtaga
    86881 tacaggaggg ccaataagtg ttcctgttgg agatgaaact ttaggtagaa tattcaatgt
    86941 tcttggaaaa ccagttgatg ggaaaccagc tccaaaatct gcacctaaat tacctataca
    87001 tagaccagca ccggcgtatg atgaactaga aactacagca gagatactag aaactggtat
    87061 taaggtagtt gacttactag ctccatatct aaaaggtgga aaaataggtc tatttggagg
    87121 agcaggagta ggaaagacag ttctaataca agagcttata aacaatattg ctaagcaaca
    87181 tggtggtatt tctgtatttt caggagtagg agaaagaaca agagaaggta acgaccttta
    87241 tggcgaaatg agtgagtctg gagttataaa taaaacagct ctagtatttg gtcaaatgaa
    87301 tgaaccacct ggagcgagaa tgagagttgc tttaactgga cttacaatgg cagaacattt
    87361 tagagatgag caaggacaag acgttttact tttcgttgat aatatattcc gtttcacaca
    87421 agctggttca gaagtttcag cacttctagg acgtatgcca tcagctgttg gttatcagcc
    87481 aacattagct actgaaatgg gtgcacttca agagagaata acatcaacta agaaaggttc
    87541 aataacatct gttcaagcag tatatgtacc agcagatgac ttaactgacc cagcaccagc
    87601 tacaacattc tctcacttag atgcaaaaac agttttatca agacaaatat catcactggg
    87661 tatatatcca gcagttgacc ctcttgaatc tacatcaagg attcttgacc ctagtatagt
    87721 aggaaaagaa cattatgaag tagctagagg tgtacaatca atacttcaaa gatataaaga
    87781 acttcaagat ataattgcta tacttggtat ggatgaatta tctgatgaag ataagttaat
    87841 agttgcacgt gctagaaaga tacaaagatt cttatctcaa tcattcacag ttgctgagca
    87901 atttacagga aatccaggtc aatatgttcc tgttaaagag actgtaagag gatttaaaga
    87961 aattcttgaa gggaaacatg atgacttacc agagtcagca ttcttatttg taggaacaat
    88021 agaagacgct gttagaaaag cgaagggaag tatgtagtca tggccagtga attttcattg
    88081 aaagttgtaa ctccaagtaa tactttttat gatggagatg ttgaaatgat agtagttaga
    88141 actacaaaag gagacaaagg ggtgttgaaa ggtcatatac catttgtttc aggtatacct
    88201 gtaggaactt taaagataaa aaaagaggga agttttaaag aggcgaaaat agcagaagga
    88261 ttcattaatg taactcaaga taaaactgtc atattgacag aatctgctga gtggatttaa
    88321 taaattaatt tagagtataa aaagtcattt tgaccaaaaa aacttttggg taaaatggct
    88381 tttttgtgtt ttggtgttaa taaaataacg ataaaattgt aagtttttag tgataataaa
    88441 ttcacaaaat gtttaaattt actgccaaat tatgttgtaa ttaataagat aaagaagatt
    88501 tagatagctt ttatgtaagt gaaaaagtct tcatataaga aatacatata aaatttttat
    88561 aaaatttaaa aaatttgctt agttgtgtag tataataaat aatataatag atttgtatat
    88621 aatattcatt acaaataatt ttaaaagtta cagttttatt ataatgatta ttaaattaag
    88681 ataagtaaca taaactaaat aataatgtgt attatcgata gtaaatttat aaattcatta
    88741 ttaattattg aaagaagtat tataaatttt ctggaggaac aatgaatata tttgatattg
    88801 gcgtagatat aattgaaatt gatagaataa gaaaggctgt agacaaaaat aataggtttt
    88861 tggagaagat atttacagat agagaaatag agtattttaa ttctaagaat tttaaagcag
    88921 aaagtatagc aggtaatttc gcagccaagg aagctataag taaatctata ggtacaggaa
    88981 ttagagtgtt taattttaaa gatatagaag ttctcagaga tgaaatggga aaaccaatag
    89041 taaaaactta taataatcta gcaaaaatgt gcatagatta taatgtttta gaaataaaag
    89101 tttcaatatc acattcaaaa gattatgcta tagcaaatgc gataaccata attaaggact
    89161 aaggagtgat tacattatga tggataaaac agcaaaagaa ataatgacaa cagatgtaat
    89221 agtagcaaaa caagatgata gtatagcaga tgtagctaat atgctaattg cagaaaaaat
    89281 aggaggtctg ccagttgtag attcagagaa tagagttgtt ggaataattt ctgaaacaga
    89341 tattcttaaa aaagaaaaat atatagaagc acctctctat ataaatctat tacaaggatt
    89401 gattttctta gatgacttaa aaaaagttga aaaagatata aaacaagtag ctgcttataa
    89461 ggttggagaa cttatgtcta aagatataat aaaggttcat gaagatgata agtttgatga
    89521 tgtagctaat gtaatgatta aaaaatcaat aaacagagtt cctgtagtag atgatgacaa
    89581 caaacttaaa ggtataattt gtagatatga tataataaaa gctttataca atgaataaac
    89641 tgaagtagga ggtgtgatgt gagaaaaaag tggaccatag tatgtattat gttcttggca
    89701 ctggttataa ttgtaatagg atgccaaaaa cgacagtcca caaaagaaga agtatacaaa
    89761 gattttcaaa agcagatttc tgatatgaat tattacagtt gtaaagctga agtagaagta
    89821 gttggaaata aaagtccaca caattatgtt ttaattcata cttataaaaa aaccgataac
    89881 tataaattag aagtaatttc cccaaaacat ttaaaaggga aaagtataga atatcaaggt
    89941 gataaaatat tagttaaaaa tccaaagatt agtgatgtag tggagttacc taatacagga
    90001 aaaaataatc aatatctttt tgtaggagac tttataaaaa actatcttca aaatgaggag
    90061 atgaaagtaa agttatccaa aggacatttg gttttagaga cttttattcc tggggataat
    90121 aaatatttta acaagcaagt attatatgta aatgctgaca caaaaaatcc tgaaaaaatg
    90181 gaagtgttag ataaagaggg agtgccaaga tttacagtaa aatacaaaga ttttgaatac
    90241 agaaactaag ggggactaaa gacatgcaaa aaataacagt gcctacatgg gcagagataa
    90301 atctagataa cttaagattt aacttaaata atattaaaaa tttattagaa gaagatatta
    90361 agatttgtgg agtaataaaa gctgatgcat atggacatgg tgcagtagaa gttgcaaaat
    90421 tgctagaaaa agaaaaagta gattacttag cagtagcaag aactgctgaa ggaattgaac
    90481 ttagacaaaa tggcataaca cttcctattt tgaacttggg atatactcca gacgaagctt
    90541 ttgaagattc tataaaaaat aaaataacta tgacagttta ttctttagaa acagcacaaa
    90601 agataaatga aattgcaaaa tctttaggag aaaaagcctg tgttcatgtt aaaatagact
    90661 cagggatgac tagaataggt ttccaaccta atgaggagtc agtacaggaa ataatagaat
    90721 taaataaatt agaatatata gatttagaag gtatgtttac tcattttgct acagctgatg
    90781 aagtaagtaa agagtacact tataaacaag ctaataatta taaatttatg tctgataaat
    90841 tagatgaggc tggtgtaaaa atagctataa aacatgtatc aaacagtgca gctattatgg
    90901 attgccctga tttaagatta aatatggtaa gagcaggaat aatattatat ggtcattatc
    90961 catctgatga tgtatttaaa gatagattag aattaagacc agccatgaaa ttaaaatcaa
    91021 aaatcggaca tataaaacaa gttgaaccag gtgtaggaat aagttatgga ctaaaataca
    91081 caactacagg taaagaaaca atagctacag ttccaatagg atacgcagat ggatttacta
    91141 gaatccaaaa aaatccaaag gttcttatta agggagaagt gtttgatgta gttggtagaa
    91201 tatgtatgga tcaaataatg gttagaattg acaaagatat agacataaaa gttggagatg
    91261 aggttatact atttggagaa ggcgaagtta cagctgagcg tatagctaaa gacttaggaa
    91321 ctataaacta tgaagtgtta tgtatgatat caagaagagt tgaccgtgtt tatatggaaa
    91381 ataatgagct tgtacaaata aacagttatt tgctaaaata aaaccatgat attataatct
    91441 gggaggcgat tttgtgtacg ataagagtaa agaaaaaata gaagttactt tacctgactc
    91501 tctgatttct gaagttgata gtatagttca gatggagaac agtaacagag aagattttgc
    91561 aaaagctgct tttcagttct atataactca gaaaaagaaa attcatatta aagaatctat
    91621 gaaaacgggt tatagagaaa tgggtcaaat taatctgtca ctagctgaat taggtatgac
    91681 agttgaagaa gtatcttctg atgataaaaa tgaagggaag attgcccaag gtgagtacta
    91741 attgtgaaat aaagcgtggt gatttatatt acgctgattt aagtccagtt gttggttcag
    91801 agcagggagg cgttagacct gttctgataa tacaaaatga tgtaggtaat aaatatagtc
    91861 ctacagttat agtatcagct ataacatctc agattaataa agccaagtta cctactcatg
    91921 tagagataag ttccaatgaa tatgggctta ataaagactc agtaattctt ttagagcaaa
    91981 ttagaactat cgataaaaag agactgaggg aaaaaattgg ttgtctagat gaaaatatga
    92041 tgataaaagt agatgatgga cttcaaataa gtttaggatt gtttactttt taaagaggga
    92101 tttgtctttt attagacaat cccttttttc ttttaaatat ctcttaaggt ttaattttaa
    92161 tgtactatgt taataaaatt aaaaataacg caaaaaaata gcataaaagt gatataataa
    92221 tatggaattg atataaaatt acgaactcaa gggaggtaat tgcatgagag accataaagg
    92281 attaaatgag attgcgcgta tcataagaag agatatagtt tcaatgatac atagagccaa
    92341 atctggacat ccaggagggt cattatcagt agttgaaata cttactgcgt tatactttga
    92401 tgaaatgaat gtggattctt caaatcctaa aatggaggat agagatagat ttgtattatc
    92461 aaaaggacat gctgcaccag cattatatgc tacattggct gaaaaaggtt attttgataa
    92521 ggaagaactg aatggattaa ggaagatagg aaggatgtta caaggtcatc cagacatgaa
    92581 aggcacgcca ggtgtggaaa tatctactgg ttctttaggt caaggttttt cagttgcatg
    92641 tggtatggct atggcatcaa agttggataa tgctccgtgg aatgtgtata ctttgcttgg
    92701 tgatggtgag gttcaagaag gaattgtttg ggaagctgct atgagtgctg cacattataa
    92761 acttgataat ttaattgctt ttttagataa taatggactt caaatagatg gagatataga
    92821 aagcgtaatg agtttaggtt caatagttga taaatttaaa gcttttggtt ggaatgtaat
    92881 tgagatagat ggacatgatt ttgaccaaat atttgcggct cttgatatag ctaaatcaac
    92941 agttaaaaaa cctacaatga tagttgcaaa aactataaaa ggcaaaggaa tatcttttat
    93001 ggaaaatcaa gcaggatggc atgggacagc tccaagtgat gaggagctag aaaaagcctt
    93061 gcttgaatta ggaggtgctg acaatgagta aaatggcgac gagagaagca tatggtaaag
    93121 cattagtaaa attgggtcaa ataaatgaca atgttgtagt attagatgca gatttatcaa
    93181 agtctacaaa aacacatgat ttttacaagt catttccaga tagatttttt aatatgggta
    93241 tagctgaaca aaatttaata ggcgctgctt gtggattatc aactgctggt aaaatacctt
    93301 ttgcaagcac ttttgctatg tttgctacgg gaagggcttt tgagattata agaaattcag
    93361 tttgttatcc aaagctaaat gtaaaaatat gtgctactca tgcaggtctt actgttggag
    93421 aggatggagc atctcatgaa agtgtggaag atattgctat aatgagagct ataccaaata
    93481 tgacagttct tgttccagct gatggagttg aaacagaaaa aattattttt gaaatagcta
    93541 agtataatgg acctgtttat gttagattag ggagaagttc agtaccagtt ttatttgatg
    93601 aagattataa atttgaaata ggaaaaggta cagtcttaag agaaggaaag gatgtatcaa
    93661 ttatagcttg tggaattatg gtaaatgaag ctctattagc acaagaaaag ttgcaagaag
    93721 aaggtataag tgctagagtt ataaacatgt cctcgataaa acctatagac aaagatttaa
    93781 tattagaatc agcaaaagaa acgaatgcta ttgtaactgt agaagaacat agcataattg
    93841 ggggattagg ttctgctgta agtgaggtag taggagagtc ttgtccaacc attgtaaaga
    93901 aagttggaat taaagataca tttggagaat ctggtactcc aaatgaattg ttaaaaaaat
    93961 atgaattaac ttgtgatgac ataataaaga ctgttaaaga agctattatt gcaaaaagaa
    94021 tgtaagagat ataaattatt catgtttgtt aataatataa tatatttaaa agagaggtat
    94081 aaagtgttat attaatttat atctctcttt tttttatatg atatcaatat atcaaaaatg
    94141 taataaaata ttcaaatttt tacaaaaata gagaaaatat tcaaatatat attgactatt
    94201 cagaaaaata tgactaatat ataatttata gagaaactaa ttttatatat aatgactact
    94261 tagataaaag aatctaaaaa aattatgaga ggaggagtag ggtataaaat cagattataa
    94321 gttttaagtt attatactaa aaagctataa aacagttatc atgtatttta tgttaaagga
    94381 aattgtaggt agtttatcga attgtttaat cagaagaaaa aatttcacat ggggaggtaa
    94441 ctgagatatg tcagaaaaag taaatgttaa aacagtcatc agtttcgcag gtgcgtatgt
    94501 agcaacagta atcggttctg gatttgcaac aggacaggaa ataatgcaat tcttctcttt
    94561 ttatggtttt gcaggtatta ttggtgggat aatttcaatg gtattattct catggatggg
    94621 tgcttcagtt atgtcaaaag gtaaagagct tcaacttaaa gaacctatca aaatttatcg
    94681 tgtttattgt ggtaagtatt taggaatatt ctttgaatgg tttggacctt tattcttatt
    94741 tggtgttttc gtagtaatga tttcaggagc aggagcaact ttaactgaat actatggatt
    94801 aaatccattt gtaggtagag taggtatggc agtagtagca ttattaactg tttcactagg
    94861 tcttgataaa ttatcaaaga tactaggtgg aatagggcct ataataataa tctttacact
    94921 tttagttggt ggtataagtt tagctaaaaa cataggtaat ataggagaag ctgcacaagt
    94981 tcttagcact gtaaagatag ctaaaccagt tccaaatgcc tatctatctg gtgtaatata
    95041 tactacttat aacacaattg ttgttatggc attcttaact ggattaggag caagtgctgc
    95101 taacaaaaaa gaagctattt gtggtggtat attaggtgga gtagcactta tggctgcagc
    95161 tataatgatg cacttagcta ttctttctga tataggaaac ttatattcaa aagcaatacc
    95221 atcattattc ttagcagata agatttcacc agtaattgga gttttattct caataattct
    95281 tatattaggt atatatacaa cagctgtacc tttattatgg tcagtaacta atagatttgt
    95341 agatgacaaa catcctaagt ttaaattaat aactttaata gcagctatat taggtcttat
    95401 aggtggcttc ctaccatttg ataagctggt tggtatttta tatccatata ctggatatat
    95461 gggcgtcata atactggttt gtgttcttta tagacagatt acaaaaacat ctggatatca
    95521 agaaggaatc agtgacaaat aagtattttt taatattgta aactcaagag ctatatgaaa
    95581 acaatgaaaa tgttttcata tagctctttt tattttttag ctcgtttatt atgaaataga
    95641 aaaaacatgt tttttagtta cattacatat atttataatt atgtataaat atatactaat
    95701 caataattta gagtctaaga taagtttata aatcatatat ttagaatata tgggaggagt
    95761 gataagattg aatgtaaagt ttaatattaa aggtataatt tatggagtta tagctcttgt
    95821 attcgttata gggggattta tattcatacc aagtatgttt gtagagaaat ctaaacccat
    95881 agattataca gtattacaaa gaaattcaat accagaaaag atattagatg tgatggataa
    95941 gtatacaaat gaagaaagag cattagcggc taaaatcgat aacaaaatat acattatagt
    96001 gactagagga aataataaat atggtataga aatggacaaa atagaaagtg tggttgagga
    96061 aggaaaggat gttttgagag tcaagataaa gtataaagat aaggaagatt cttttccata
    96121 catagttgtt gaaacaaata tgagtgaact tccagataga atagaattaa atgcgacgaa
    96181 ataagaaggt attttataat taaataactg catattgtac aataaagtca tggtttttat
    96241 taatgtaagt aaattgtgta tggttcttat tgttgtaaaa gccatgactt aaaatgctta
    96301 taaagtaaga tattaacagt agtatttatt actacattaa atggggtctg gaaatactta
    96361 atattctaac tatttggagt gataattcta gtttcagtaa ctatgtagtc taattgtgca
    96421 tcatgagttt cttttggaat agaatcgaat atctggaagt caaatgctat gccgatagtt
    96481 atagcatctt ttcttaagtt ttctaaaaat ctatcataaa atcctccacc atatcctaat
    96541 ctatagcaat gtatatcata tgctacagct ggtacaatta caacatctaa ggccttaata
    96601 tcaattgtag gtgatttttc gttaggttct cttattccat atgctccaat tctcaatccc
    96661 tcttttaaat ctgttatttg agtagggatt aatgtatgat tttcttttat tgtgatggga
    96721 cttgatacta ttttctttag agacaaaagt tttttgatta aattatctgt ttgaacttca
    96781 ttgttaaaat ttagataaag cattatatta gttgcatttc ttatatcttc catttgaagt
    96841 aatttttcag ttattaaatt tgaattacta agtacaaagt ttgcattctt attttttctt
    96901 gattcaataa ctttttttct aaattctttt ttcataaaat aattcctccc ttaatatgtt
    96961 ataatgtgta cagcaattgt atttaatata aattttagta taatttattg taataaagca
    97021 atatatttgg gtaaatgtaa taaaaataac agctagtcat ataataataa tagtttatgt
    97081 aaaaggagat gtatcttgtg atttcaaaga agaaagctat ttttttaggg gtgattttag
    97141 taattataac agctatggtt acatcagcat ttcagttgac attggggaat aaagttgtaa
    97201 tatcgaaaga actctatgaa gactataaaa aatatgataa gttgttagga ctagaatcta
    97261 ttataaaaca agatttttat aaaaaagtat cagatactga tttggtaaat ggagcagcaa
    97321 aaggtctatt tctaggaaca aatgataagt attctggtta ttatactaaa gatgaaatgg
    97381 agaatctaat taatgatagt gaaggttctt atgttggtgt aggtatgtat ataggggcat
    97441 caaaagatgg tggcttagta gtagttccca tgaaagattc tccagcagag aaagccggtg
    97501 taaaatcagg ggataaatta gtaaaagtca atggaaaatc tgtctcatat aaaaattcag
    97561 atgaagctgt tcgtatgatg aagggtaaaa aaggtaaaac agttgaactg actatattaa
    97621 gagaagataa acaattgaac tttaaagtaa aaacagacca gataattgag aagagtatag
    97681 aaagtaaagt aattgataat gatttaggat atatagaaat tacccaattt atatcaagta
    97741 catatactga ctttgataaa gctttaaaag aattaaaagc aaaaaatatc aagggattag
    97801 taatagacct tagaaataat cctggtggaa tgctagatat atgtaaagaa gttgctgatg
    97861 agttaattgg tgaaggaact atagtatata caaaagataa taagggaaat actgagtact
    97921 tgaaatctga taaagaaaaa ttgggacttc caatagtagt gttaactaat ggagaaagtg
    97981 cttctgctgc tgaaatatta acagctgcaa tcgttgataa taaagaaggt atttctgtag
    98041 ggactacaac ttttggtaaa gggttggtac aatcagttgt aagattgaag gatggaacag
    98101 gatacaagct aactactgct caatatttta cacctaatgg ggattatatc aatgaaaaag
    98161 gtataaagcc tactattgaa gagaaagatg aaaataaaca gttagatgta gcaactaagt
    98221 ggcttagaga acagattgat aaataggatt taatttttat aaacttatac tataaaataa
    98281 agaacattat aaaagagatg tctcaaaatg aactttttag ttcatgaggg agtctggaaa
    98341 tatttatgtg tattctttaa ataaaaattt attttttaag tgataattaa atttttacag
    98401 acagggctat ggttttaaaa atcatagcct tattttgata ttttttaaaa acatattgca
    98461 acccagttgt tccaaatatt atatcattta cttttaaaat atcgcaaact gcatattata
    98521 tattcattat aatataattt atctaactag aacctcagtt tactaattaa attagatgca
    98581 tagaataact tttttaaaca ttgaaaataa attaaactct aaacacattt aaatataaat
    98641 ttatatatca ttttgacttg gttaaacaat ttcatactta tcgatataaa aataggttat
    98701 aatataaatg ttcattatct tataacctat tttatataat ttattcctaa aacatgtgaa
    98761 tgttttttat gatagtttga agaaattata aaatcacttt ataattgaat attatatata
    98821 aattaattta aatatagaaa ggaaaatata tgtacaacaa tatcaaaaat attttaaaag
    98881 aaaatcatat aacgtttgaa gaatatgacc atgaacctat tttatcattt gaaaaagcta
    98941 aagaaataag agaaaaatta caatatactg gagtagaaag taaaagttta tttataaaag
    99001 ataggtctgg aaattactat atatttgtaa ccacagaagg aaaaaaatta gatccaaaaa
    99061 ctataaaaaa tgttttaggt ggaaaagtat ctatatgttc aaaagaagaa ttaacacaaa
    99121 aaacacaatg tttgcctgga tgtgtatctc cttttggata tgagaaaaat atctctcttg
    99181 taatagatca agatatattt aaccatgata aaataatctt ttcaccaggg ataccagaaa
    99241 agacatttat tattacagct agtggattta aaaaagtaat aaataatata aacaatacaa
    99301 cttataaata tatatatatt aatgaatttt aatcataatt gtaaattaga gaaaataaca
    99361 ttagaaatca tgcttaacta tagtgtaaat aaattatatt caacacaaaa gattgatact
    99421 attattacaa tagttactgt taagctcaaa ttcaatgtat tatgagttat tatttttaaa
    99481 ttttaattac aaaagcaggg tagttttaac tactctgctt tatatttaac ataaaatata
    99541 taaaatcaac aaaatgattt tagtctatct attagatatt cttcgctctc aatgcttaga
    99601 acttttagat atatacaata agtgctttca tttgtaaaaa tttctatagt attatcttgt
    99661 ttatttacac ctacaatatc tttaaaattg atatgatttt tagtattttt acatttgaaa
    99721 atatatctgt tatcaattaa aatctcatca ttagtaattg aaatataggg tttatctcat
    99781 gctttattaa aaatttatat gtttgttcaa gataaaaact tacaaataat aatagaaaag
    99841 ttactttaaa tgtacatatt atcgaaaata ttatcacaaa aatacttata atatatattt
    99901 gtttaaaata acctctatcg caatagttta ttaacgatat ggaaataaaa gtgttaaatc
    99961 agattttgga gaaataactt taaatatttc cagactcttt ttagacagcc tccataacct
   100021 tatattgaac ctatttatta actttgtact tacatatacc acttatataa gtttcgtata
   100081 tattaaagtc cttatctgtt actataaaat ctgcctccat accttgatgt atggagccac
   100141 aaacattatc aatattacaa cttaaagcag ggatatatgt agccattttt attgcatctt
   100201 ctaaagtggc tatgttccaa tcaactatat ttctcacagc cttgtccata gttaatacac
   100261 ttccagctaa actcccacta tttaatcttg caacactatt ttttacattt acattaaact
   100321 taccaagagt atattttcca tcctccatag caccagctct catacaatct gtaattaaag
   100381 ctatgttatt actagactta gcatccatca ttattttaac agctattggg cttacatgat
   100441 gaccatcaca aattaattca gcataagtat cttttagtac catagcagcc ccaaccattc
   100501 caggttctct atgatttaaa ggactcatcc cattatatgt atgtacaaac actttagcac
   100561 ctgaattaac gacttgttta acttgctcaa atgtagctga actatgacca agagaaactg
   100621 atatattatt tttagttgca tatcttgtta attctattga acctttacgt tctggagcaa
   100681 ttgctatttt tcttattaaa tttttcgatt ttttttgcca taattttaat atattgatgt
   100741 cagggtcttt aaaatatttt tcattttgag cgcccttata tttttctgta aaaaatggac
   100801 cttctaaata aatcccttgt atttttgcac cctcaactcg attgaagcta ttgcttatat
   100861 tttctacaga atcacttagt gtatctacag aatcagttaa agttgtaggt aggaaagatg
   100921 ttactccata ttttaataag ccttttgaaa ttgtgtttat tgcttcaaat gtattgtcca
   100981 tgacatctgc acctaaaaga ccgtgaatgt gagtatcaac taagccagga gcaatatatt
   101041 tacctgaaaa atctataaca ttatcagtaa cattaggtct ttcttgttga aattcaccga
   101101 atttgccatt aattatacta aggtaacctt tttctttgat tccatgtttc ataaaaaatt
   101161 tatctgcata aataaaactt ttcaaaataa cctccttata attatattat cattgtggca
   101221 ttgacgctta taatatcatt ctgttttctt gaaagttatt taacttacta tcttacatgt
   101281 cttactctat atacaaattt atcacttcta gcgacactca aagtaaattc aataactata
   101341 ttgtcatcat tatatgtggt acgaagtaat ttcagacaag gtgaatctcc tggtacttcc
   101401 aataacttag cttccttatc cgagattatt ccagctgaaa attcttcatc agcaacttta
   101461 ataatctcgc cataatcttc tctaaatatt tcatataaag gtttttgttc gattttttct
   101521 tttgtcaatc ctataaattc tttcatagga agatatgttc tttcaagcat cataggaagg
   101581 tcatcagcaa gtcttaaacg cattattttt atcatttttt caccaggagt tatacctaat
   101641 ttttcagaga aatactttgt actttctact acctcaaatg ataatacctt tgtacctggc
   101701 ttttttcctt gtgttctcat ataatctgta aaactataac tttccattaa gttttgagaa
   101761 ttttgactta gtgcagcaac aaaagttcct tttccatgtc ttttgtatat atatcccata
   101821 tgctctaact cagccatagc tagtctaact gtggttctac ttactccata ttcatcacaa
   101881 atttttcttt cggaaagcat tctatcgttt ggttccatct cattttcaat cttagttctt
   101941 aatatctcaa caagctggtc atatattggt cttttatcat ttctttttat cataaaaata
   102001 tagtcctttc taagtggtta ttaccacaat ttgattatag aatatagaat tatttatgtc
   102061 aatgaataaa gtttaatcaa aattcaataa gtaaggctat taatttatta taaaattatg
   102121 gtacattgac aaaacaagta aaataattta tattcaaatt ggtaataacc actttggata
   102181 tattatatga ggtatttttt attttacaga ttaatttatt gtttagaaaa gtaaaaatta
   102241 ataatgagga gaatctagta tgattacagt aataacattc aacccatcta tagatagaat
   102301 gtatagagtg aataatataa acataggtga ggtgcaaagg gtagtaagta agaatgcaac
   102361 tgctggaggt aaaggattaa acgtatctaa ggtatgtaaa attttaaaag aaaatccttt
   102421 agctatggga tttctaggcg gatttaatgg agaatttata aaaaatgaac ttagaaaact
   102481 agatatacaa aataaattta caaaaataga gcaggaaact agaaattgct taaacataat
   102541 tgaggacaat aaagttagta cagaattttt agaaaaagga ccaatagtag aaaaaagcga
   102601 tttagagaga tttgaaaatg atatagagga agtactaaaa aatactaaaa tactggtagc
   102661 ttctggtagt tattgtgaga atatgccgat agactattat gaaaagatag gaaatatatg
   102721 tagagcaaat aatataaagt ttatattaga tacatcagga gaagcattaa aaatagcttt
   102781 aaaatcaaaa ccatatttaa taaagccaaa tatagatgaa ataagacatt tattaggaat
   102841 aaatatagaa tcaagagagg aaattatctt gtcaggaaaa aaacttattg aaatgggtgc
   102901 agagaatgta tgtatatctt taggaaaaga tggtatggtg tatttaaacg aagataacgt
   102961 atattatgta aaagtaccag ttgtaaagtg tataaatact gtaggtagtg gagatagcac
   103021 agtggctgga tttagtgtag gtattttaag gggttataaa atagaaaatt tacttaaatt
   103081 tgcaaatgct tgtggtataa gtaatgcttt aaatatggaa actggatttg tagatttggg
   103141 agaaattgat aaatatcaag atttagttga agtaaaaaag ttaagttaaa tacatttaat
   103201 gggaatataa taaatttaat gttgttgtaa tagattttaa ttttaatata aaagatatag
   103261 tcttatataa aaataggtta gaaaaattca tttttaacct atttttataa taaatatcta
   103321 ttttactgaa tttataattt tctctagtaa tctttgagcc ttgatacact cttctccact
   103381 gatagctggt tttatattat tttctataca atcaataaaa tgatttgata tatcctcaaa
   103441 acccttttgt tttaatatat ttacccatgc accactttgc tttaaagtta ggtttccacc
   103501 ttcttctatt tccagtacat taagattctt aactctttga atcttaccct ttgaaagaat
   103561 ttcaacttgt tctaactttg ttccagaatc tctatgcata gaggtgttta tagagaaatt
   103621 tttaccttta agtttatgac ttacaaatat aagattctta ttgtctgtta aaaataaatc
   103681 ctctccacta atttcaacat cttcatttgc taaccataaa gcagtgtcaa taacatgtat
   103741 atagtcatct ataagagtac tgtcgaattt aacatttctt aaggaattta atccatgttt
   103801 acatatattg atagatacaa tttctgtagc attattttta atttctttgt acataggaca
   103861 gaatctacgg ttgaatccaa ccattaaatt taagtttttt ttcgtactta attcgataag
   103921 ttcttctcct tgacttactg ttgaagcaag aggtttgtct acatatacat gaacacctaa
   103981 atttaataat atttttataa tttcgtagtg tgtttctgta gagctatgta gaaaaataca
   104041 atcacatttt ttagctaggg attcaataga atcgaattgc atgattctat aatcactaca
   104101 gactttttcc cttttaactt tatttggagt aaaagctcct acaaactcaa atctctcaga
   104161 cttagttaat ataggtaaat atgccttttg agctatggag ccaagaccta tcattcccat
   104221 ttttatattt ttcataatac tttctcctaa ggtttttata tttacagaat tgattataat
   104281 tcagacacat aaatttgtaa actttgtatc aaaaaataaa tttaaagctt atataagtaa
   104341 attagcgaaa taagaaatat tgataaagta attttgtagg atatctataa aattgattga
   104401 tataaatatt ttatgtattg acaaaaaatt caaattaatt tatatttgga ttagtggtaa
   104461 ttaccacttt ggtctgaaca agtttttatc gtaaaataaa aatactaaag tatatagagt
   104521 atgattttat taaattttta aagataaact ataaaattaa ttaaaatcta tagtgtatat
   104581 tttatagtga gtttaagtag tactaaaaat ctaagcaaaa aattataaaa gagtatattt
   104641 ttttatagtt taaagtggtt ataacctcat taccaaatgt aaacgttaca agtatgaaag
   104701 gagaatgaaa atgtttaaat tagaagaaca aaaactaaaa gatttaggtg caataataac
   104761 aactaatgaa ataaaacaac agcctgaatt atggttagag acttatgaaa tatataaatc
   104821 taacaaagaa aaattaagta gattcataga tacaataagt aataatcatg ggcagtttag
   104881 agttatattt acaggtgctg gaacttcagc ttacatagga aattcaatac ttccttacct
   104941 taaaaataaa aatgacataa gaaaatacat atttgaagca atacctacta cggatatagt
   105001 ttcaaaccca tatgattact taaaaaaaga tataccaaca ttattaattt catttgcaag
   105061 aagtggaaat agtcctgaaa gtttggctgc tttaaattta gggaataaga ttgtagataa
   105121 tttttatcat ttagctataa cttgtaatcc agaaggagaa ctagctaaga tgactaaaaa
   105181 cgacgaaaat aattacttgt tattaatgcc atcaaaatca aatgatgaag gctttgccat
   105241 gacaggaagt ttttcgtgta tgatgttatc tgctatgtta atattcgata gtttagaaga
   105301 tgatgtagaa aagtcatata taaatgcaat agttgaaatg ggaagaaatg ttatagatag
   105361 aaaagatgaa atacatgagt taataaataa agattttgat agagttgtat atttaggttc
   105421 aggaggatta ggtggtttga cacaagaagc acaactaaaa cttttagaac ttacagcagg
   105481 aaaaatatct acagtgtatg attctcctat ggggtttaga cacggaccaa aatcatttat
   105541 tgatgaaaat acacttgtat ttgaatttgt atctaactgt ttatatacaa gaaaatatga
   105601 tttagatgtt ttagaggaga taaaaaggga taaaattgct aaattcactt gtgcagtgtc
   105661 agtagaaaat gaaaataact tctcaggaac aaagtttgag tttaaagaaa agtataataa
   105721 attaccagat gtgtatcttg caatgccata catattgttt gcacaaacta tagcattgtt
   105781 tgtatcggta aaggttggaa ataagccaga tacgccatca gctacaggaa cagtaaatcg
   105841 agtagtaaaa ggtgtaacaa tatatgaata ttaaaagata agattaaaat attataacta
   105901 aataatttgg gagggtatca atgtggcatt agtaacaaca acatcaatgc ttaaaaaggc
   105961 acaagatgaa gggtatgcag taggagcatt taatgtagaa aatatggaaa tggtaattgc
   106021 tgttatagaa gctgctgagg agttaaattc tccagtaata ttacaaacta ctccatctac
   106081 tgtaaaatat gcaggtcttg attattatct agcaaatgca aaggttgctg cagaaagagt
   106141 aaagatacct gtagcaatgc atctggacca tggttcaagt tttgaccttg caatgaaagc
   106201 tttaagaagt ggatatacat caataatgat agatggttct catgaaagct ttgaaaataa
   106261 tatatctata tctaaatcag tagtaaaagc atgtagtcct tctcttatac cagtggaagc
   106321 agaacttggg aaagttggtg gaaaagaaga tgatttagat ggcgggaata gtaatgatta
   106381 tacaaatcca aaagaggcaa aagagtttgt cgatagaaca ggaatatctt cattggctat
   106441 tgctataggt actgcacatg gattgtataa gggtgaacct aaaatagatt tagataggtt
   106501 atcagaaatt aaaaatgtag tttcaatacc tttagtattg catggaggtt caggaataca
   106561 tgatgatatt ataaaagaag ctataaaaag aggtattgca aaagttaact atgcaactga
   106621 attaagaatt gcatatagta aaggggttaa aaaagtttta gatgaagatt ctgaggttat
   106681 agaccctaaa aagtatggat tagcagggct tcaatcagta aaggattttg tgaaagaaaa
   106741 gattaaagtg tgtgggtctg taaatagagt ataaaaataa aatatcctaa atttatttta
   106801 gataagttta ggatatttgt tatatgaaaa atttatatta attatatgta aatggtgtat
   106861 ttttttcatt taattgatga aa
//
LOCUS       DAMJWS010000003        92476 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMJWS010000003 DAMJWS010000000
VERSION     DAMJWS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 92476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.003"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(125..805)
                     /locus_tag="PC399_000207"
     CDS             complement(125..805)
                     /locus_tag="PC399_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591086.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(957..2864)
                     /locus_tag="PC399_000208"
     CDS             complement(957..2864)
                     /locus_tag="PC399_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2591087.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(2865..3617)
                     /locus_tag="PC399_000209"
     CDS             complement(2865..3617)
                     /locus_tag="PC399_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591088.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(3933..5087)
                     /locus_tag="PC399_000210"
     CDS             complement(3933..5087)
                     /locus_tag="PC399_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2591089.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(5090..5557)
                     /locus_tag="PC399_000211"
     CDS             complement(5090..5557)
                     /locus_tag="PC399_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2591090.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(5571..6059)
                     /locus_tag="PC399_000212"
     CDS             complement(5571..6059)
                     /locus_tag="PC399_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591091.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(6073..7404)
                     /locus_tag="PC399_000213"
     CDS             complement(6073..7404)
                     /locus_tag="PC399_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2591092.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(7418..8983)
                     /locus_tag="PC399_000214"
     CDS             complement(7418..8983)
                     /locus_tag="PC399_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2591093.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(9153..9938)
                     /locus_tag="PC399_000215"
     CDS             complement(9153..9938)
                     /locus_tag="PC399_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2591094.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(10105..11304)
                     /locus_tag="PC399_000216"
     CDS             complement(10105..11304)
                     /locus_tag="PC399_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF2591095.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEEMSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(11826..13163)
                     /gene="xylA"
                     /locus_tag="PC399_000217"
     CDS             complement(11826..13163)
                     /gene="xylA"
                     /locus_tag="PC399_000217"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF2591096.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(13259..14809)
                     /gene="xylB"
                     /locus_tag="PC399_000218"
     CDS             complement(13259..14809)
                     /gene="xylB"
                     /locus_tag="PC399_000218"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF2591097.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            15017..16177
                     /locus_tag="PC399_000219"
     CDS             15017..16177
                     /locus_tag="PC399_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2591098.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(16333..16737)
                     /locus_tag="PC399_000220"
     CDS             complement(16333..16737)
                     /locus_tag="PC399_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2591099.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(16758..17240)
                     /locus_tag="PC399_000221"
     CDS             complement(16758..17240)
                     /locus_tag="PC399_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2591100.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(17260..18069)
                     /locus_tag="PC399_000222"
     CDS             complement(17260..18069)
                     /locus_tag="PC399_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2591101.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(18062..18841)
                     /locus_tag="PC399_000223"
     CDS             complement(18062..18841)
                     /locus_tag="PC399_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2591102.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(18841..20919)
                     /locus_tag="PC399_000224"
     CDS             complement(18841..20919)
                     /locus_tag="PC399_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF2591103.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(21718..22239)
                     /locus_tag="PC399_000225"
     CDS             complement(21718..22239)
                     /locus_tag="PC399_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2591104.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            22405..22971
                     /locus_tag="PC399_000226"
     CDS             22405..22971
                     /locus_tag="PC399_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2591105.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTIGTVLGAVV
                     LGPFWSALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(23188..25251)
                     /locus_tag="PC399_000227"
     CDS             complement(23188..25251)
                     /locus_tag="PC399_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF2591106.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(25632..28967)
                     /locus_tag="PC399_000228"
     CDS             complement(25632..28967)
                     /locus_tag="PC399_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2591107.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKTETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(29017..29445)
                     /locus_tag="PC399_000229"
     CDS             complement(29017..29445)
                     /locus_tag="PC399_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591108.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(29435..29815)
                     /locus_tag="PC399_000230"
     CDS             complement(29435..29815)
                     /locus_tag="PC399_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF2591109.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYKSKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(29848..30156)
                     /locus_tag="PC399_000231"
     CDS             complement(29848..30156)
                     /locus_tag="PC399_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591110.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(30201..31361)
                     /locus_tag="PC399_000232"
     CDS             complement(30201..31361)
                     /locus_tag="PC399_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2591111.1"
                     /translation="MEYRLNEVFEFIRNGASIKQFDTKFGYPITRIETIANSYIDTNK
                     MGYADIKELGKLEDYLLKNGDILMSHINSEKHLGKSAIYEDINKKIIHGMNLLCLRPD
                     KEVCYPKYLNYFFNSNEFRRQLPRIIKKSVNQASFSVNDLKNLKVDLPEMEAQKDISK
                     VLDKAQELIDKRKEQIEALDELVKSRFIEMFGNFKEQKFVLLKDCTNFIDYRGKTPIL
                     SENGIRIINAKSVGNGVFKYINEYISENTYNSWMKRGFPVAGDVLFVTEGHTFGNVCR
                     IPKDLKKFAMGQRIITIQGDKKILNNAFLAQYMQTKAFRVEIDKYKTGSSAQGIRSKE
                     LQKILIPVPSIELQNKFEEFVNQVDKLKVGIKTSLKELEDNFNSLMQKAFKG"
     gene            complement(31365..32828)
                     /locus_tag="PC399_000233"
     CDS             complement(31365..32828)
                     /locus_tag="PC399_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2591112.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            complement(33337..34206)
                     /gene="gatY"
                     /locus_tag="PC399_000234"
     CDS             complement(33337..34206)
                     /gene="gatY"
                     /locus_tag="PC399_000234"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2591113.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(34226..36142)
                     /locus_tag="PC399_000235"
     CDS             complement(34226..36142)
                     /locus_tag="PC399_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF2591114.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGL
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(36145..37065)
                     /locus_tag="PC399_000236"
     CDS             complement(36145..37065)
                     /locus_tag="PC399_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF2591115.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICENKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(37167..37919)
                     /locus_tag="PC399_000237"
     CDS             complement(37167..37919)
                     /locus_tag="PC399_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2591116.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTVNKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(38311..39756)
                     /locus_tag="PC399_000238"
     CDS             complement(38311..39756)
                     /locus_tag="PC399_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2591117.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKKRYKKDSFYWYQNVIRTNGEELKEK"
     gene            complement(39778..40131)
                     /locus_tag="PC399_000239"
     CDS             complement(39778..40131)
                     /locus_tag="PC399_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2591118.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(40160..41407)
                     /locus_tag="PC399_000240"
     CDS             complement(40160..41407)
                     /locus_tag="PC399_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2591119.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFTVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(41415..41732)
                     /locus_tag="PC399_000241"
     CDS             complement(41415..41732)
                     /locus_tag="PC399_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2591120.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            41974..43887
                     /locus_tag="PC399_000242"
     CDS             41974..43887
                     /locus_tag="PC399_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2591121.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     DLFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(44010..45164)
                     /locus_tag="PC399_000243"
     CDS             complement(44010..45164)
                     /locus_tag="PC399_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF2591122.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKDTIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(45424..48039)
                     /gene="mngB"
                     /locus_tag="PC399_000244"
     CDS             complement(45424..48039)
                     /gene="mngB"
                     /locus_tag="PC399_000244"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2591123.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(48183..50108)
                     /gene="mngA"
                     /locus_tag="PC399_000245"
     CDS             complement(48183..50108)
                     /gene="mngA"
                     /locus_tag="PC399_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF2591124.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AVLLIITRKQKNQRKIH"
     gene            complement(50238..50963)
                     /locus_tag="PC399_000246"
     CDS             complement(50238..50963)
                     /locus_tag="PC399_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2591125.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEVAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(51226..51996)
                     /locus_tag="PC399_000247"
     CDS             complement(51226..51996)
                     /locus_tag="PC399_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF2591126.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(51998..53425)
                     /locus_tag="PC399_000248"
     CDS             complement(51998..53425)
                     /locus_tag="PC399_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2591127.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            53816..54538
                     /locus_tag="PC399_000249"
     CDS             53816..54538
                     /locus_tag="PC399_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2591128.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            54735..56402
                     /locus_tag="PC399_000250"
     CDS             54735..56402
                     /locus_tag="PC399_000250"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2591129.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYYLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(56498..57817)
                     /locus_tag="PC399_000251"
     CDS             complement(56498..57817)
                     /locus_tag="PC399_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2591130.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRQTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(57858..58604)
                     /locus_tag="PC399_000252"
     CDS             complement(57858..58604)
                     /locus_tag="PC399_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2591131.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(58848..60857)
                     /locus_tag="PC399_000253"
     CDS             complement(58848..60857)
                     /locus_tag="PC399_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2591132.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(61251..62684)
                     /locus_tag="PC399_000254"
     CDS             complement(61251..62684)
                     /locus_tag="PC399_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2591133.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(62771..64687)
                     /locus_tag="PC399_000255"
     CDS             complement(62771..64687)
                     /locus_tag="PC399_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2591134.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGVFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(64761..65621)
                     /locus_tag="PC399_000256"
     CDS             complement(64761..65621)
                     /locus_tag="PC399_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2591135.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(65972..67159)
                     /locus_tag="PC399_000257"
     CDS             complement(65972..67159)
                     /locus_tag="PC399_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2591136.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(67173..68564)
                     /locus_tag="PC399_000258"
     CDS             complement(67173..68564)
                     /locus_tag="PC399_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2591137.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(69159..70859)
                     /locus_tag="PC399_000259"
     CDS             complement(69159..70859)
                     /locus_tag="PC399_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2591138.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(70831..72234)
                     /locus_tag="PC399_000260"
     CDS             complement(70831..72234)
                     /locus_tag="PC399_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2591139.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(72498..72989)
                     /locus_tag="PC399_000261"
     CDS             complement(72498..72989)
                     /locus_tag="PC399_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2591140.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(73015..73827)
                     /locus_tag="PC399_000262"
     CDS             complement(73015..73827)
                     /locus_tag="PC399_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2591141.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLSKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(73840..74991)
                     /locus_tag="PC399_000263"
     CDS             complement(73840..74991)
                     /locus_tag="PC399_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2591142.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            75261..75902
                     /locus_tag="PC399_000264"
     CDS             75261..75902
                     /locus_tag="PC399_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF2591143.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            75928..77472
                     /locus_tag="PC399_000265"
     CDS             75928..77472
                     /locus_tag="PC399_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2591144.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(77672..78298)
                     /locus_tag="PC399_000266"
     CDS             complement(77672..78298)
                     /locus_tag="PC399_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2591145.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(78433..79149)
                     /locus_tag="PC399_000267"
     CDS             complement(78433..79149)
                     /locus_tag="PC399_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF2591146.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDKAATKFGLE"
     gene            79575..80789
                     /locus_tag="PC399_000268"
     CDS             79575..80789
                     /locus_tag="PC399_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2591147.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            81409..82737
                     /locus_tag="PC399_000269"
     CDS             81409..82737
                     /locus_tag="PC399_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591148.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(82865..84055)
                     /locus_tag="PC399_000270"
     CDS             complement(82865..84055)
                     /locus_tag="PC399_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2591149.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSKNGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDVAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQSVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(84161..85540)
                     /locus_tag="PC399_000271"
     CDS             complement(84161..85540)
                     /locus_tag="PC399_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2591150.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(85571..86779)
                     /locus_tag="PC399_000272"
     CDS             complement(85571..86779)
                     /locus_tag="PC399_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591151.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIIPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            complement(87804..89258)
                     /locus_tag="PC399_000273"
     CDS             complement(87804..89258)
                     /locus_tag="PC399_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2591152.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKIGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            complement(89278..91149)
                     /locus_tag="PC399_000274"
     CDS             complement(89278..91149)
                     /locus_tag="PC399_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2591153.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(91139..91999)
                     /locus_tag="PC399_000275"
     CDS             complement(91139..91999)
                     /locus_tag="PC399_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2591154.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKASDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
BASE COUNT        30370 a        16393 c        10005 g        35708 t
ORIGIN      
        1 tctaaatata ttaactctga atatttattt ttaaaaatat ctatagcaaa taagtagata
       61 tttattatac taactacaac taaaggtaga gcaatatact tagacctacc tttcaaatat
      121 tttactagca ttgtattata tatcctatcc ccttcttagt ttttataaaa tcatcaaaac
      181 ccagttcttt aactttactt ctcaatctat tcatatttac agtaagtgca ttgtctgtca
      241 caaactcgtc attatcccat agagtcatca taagactttc ccttgataca accctattct
      301 tattttttaa taataatgtt aaaattttta tttcgttttt agtcaactct atacttttat
      361 ccttatactt atgttttata atccctttcc ccacatcaaa catcaaatct tcacaataca
      421 aaatactatt agaatcacta taattataga ctcttcttat tataccattt attttggtaa
      481 ccaatatttc tatagaaaat ggtttggtaa tgtaatcatc tgctccatta tttatgccca
      541 ttattaaatc catatcagaa tctcttgatg ataaaaatat taatggtaca tttgaaatag
      601 ctcttatttt ttcgcaccag taaaatccat tatataatgg taaatttaca tctaaaagta
      661 ctacatctgg ttttttatct ataaattcat ctaatactac ctcaaaatca tcaattaatt
      721 ctactttaaa tccccatttt tcaagtgcta acgcaatctc ttttgataaa tcaatatcgt
      781 cctctactaa taaaattcta tacatttgta tattctccat tatcctaata tttcactatg
      841 ctatgaatta ttaatatcat aacatatttt atatgaattt attacccaaa ttatttagtt
      901 aatcaacaag gagactctta ttacaagaat ctcctttctc tttattaata tatcaactaa
      961 tttttataca ctacactctt aaacttatta acagatactg ctccatatat tatgaatatt
     1021 atcaaacata tcactaaact tagtatatat ataccaaata aatcggtcat caataaatct
     1081 cctgctaatc tcaatgcaaa ataactagat atagttgcac ttaacattgg caaagtataa
     1141 aaaatagcta cttctttaag tatagctcta tttatatctt tttctgtagt acctattttt
     1201 aacaaaatct tatatttctc tttatcttca tatgcatcac tatatatttt catatatatt
     1261 atacttccag ctacaattgc aaagactaag aataaaaaca caagcactac atatgcaaac
     1321 ttcatccatt gactctcacc ttgagcttta tatccatttt gaatataccc attttttaaa
     1381 cttaaattct tttcaatatt gttgaacatt acttgactat tttcttcttg aaataccttt
     1441 gcaccataaa aatacacttg ttgaccctta tctcttagtt taaaatatgt atcatcattt
     1501 accactataa tactttgatt aagtcctgtc cctagtactg gaacttttac ttctcccctt
     1561 gaaatattat ataactgacc atcaagtttt atcttggctt cttttgtagc tattgatgct
     1621 atcgtttgag ggctttttaa ctctattaca ttatttccac taactaatct ttcaccatac
     1681 tcttttaatt ctttatcatt accattcact ttaagagtat tacaaaaatc actatatttc
     1741 attatagtat ctgttgtatc ataagcttct tttatatctg ttttctttaa aattgttagt
     1801 tctggattta ctgagtactt tatcttatta gtatctttta ttatattctc tatttgtttt
     1861 ttattcactt catctttatc cattgaaact atgctaatag tgtacacatt tctttgttct
     1921 tgggcattct tatgtatatg cttcatagca actgaagcac caagagttga tattgttgta
     1981 gttataatta tagctattat agcataagtt ttatagtttt tatttaatcg gtagtttata
     2041 ttgtttattg caattatatt atttccatta aataatattt ttttattttt gattataata
     2101 tcaaatataa caggcataac tgatttgaat aatccataag tacctactat tattatacct
     2161 ataacaggta gtacactagt tatatctcca gttcttatat tggtagaaag atagtagcca
     2221 tatcctatta ctataattga tactatagct aatatatata tacctattcc tacctttggt
     2281 attttttctt gttttttaga tatattgatt agatttatta cactacttct acatattgtt
     2341 ataaaacctt ttacagccat tattataaat atagaaataa agattaaagt agcatatagt
     2401 atagcttcta tactaagtcc attagatacc tttatattaa atccagataa ctttattatc
     2461 actacttgaa aaaatctaga tgttattatc cctataatta ttcctgttat acaagaactt
     2521 attcctataa gcatattttc tacaaaatat atttttccta tagtatataa atctaatccc
     2581 ataaaagaca atattccaat ttctttagac ctattcttaa aaaatatatt tgttgcatac
     2641 catataaaga aaaataaaaa tactgatagt atcattatta cagctactaa cactgatagt
     2701 gtaaattttt tattttcctc ttgtaacaca tttatagcat caccatatct taataattca
     2761 aaattagtta gcacaactac tgaaaaaacc atagatataa aaaatgctag ataagttctt
     2821 atgttactct taaaattatt ataagcaatt ccaagtatac tcacttacat ctcacctccc
     2881 atagaggcta gcatatccat aatttttcca taaaattctt ttctactatt tcctttgttt
     2941 aatttacact ttatatttcc atcatttatc atatatactt catttgaata acttgctgtt
     3001 agagggtcat gtgtaatcat tataatagta gcgttatttt ctctgtttat tttttctaat
     3061 gattctaaaa gttcatatgc tgatttagag tctaaagcac cagttggctc atcagcaaat
     3121 atgactgttg gatttgtaat taatgaccta gctgctgcgc ctctttgttt ttgtcctcct
     3181 gataactgat atggatattt atctaaatga ttttctaatc caaatttttt agctatagaa
     3241 aataccttag cttctatctc cttacttttc ttttttccta atgctagagg aagtgctata
     3301 ttgtccatta gtttcatatt gtctaataga ttatagtctt gaaatataaa ccctattttt
     3361 tctcttctta attttgatag ttcattattt tttacttttg atatattaat tccatctagt
     3421 attatttctc ctgaagtttg tttgtcaagt gtagacatta aatttagcaa tgttgtcttt
     3481 cctgcaccag atggacccat tattgatata aaatcacctt cattgactgt aagagatata
     3541 ttttttaata catttgtttt aaatcctttt attccataat cttttgatat attttttatt
     3601 tttaatattt ctttcataac aatagcctcc agttgctaat tttttaatat atatttattt
     3661 taaaggtttt atataaataa atccttaagt aaatattaca ttttaactgt tagcattaca
     3721 cttttgtaac atatggaaga ctattataat tatagtagaa atatcttaaa ataaaagcat
     3781 gatttacttc tttgtataaa caaagaaaag aagctcctaa gaagcctcta atctatacaa
     3841 ttattcttct ctataaaaaa ccaaaataca cattgattgt ggcaaataca gatactataa
     3901 acaagtgata ttactactat atattaaaac aattattcct gaagataatt caataagact
     3961 atcaagcaat aagctaagca tccaaaaaga cttaatacca ttaatgtata attataccca
     4021 atattaatgc ttaaaaaacc agcaacagtt ggaataatca gagatgaacc tcccataaat
     4081 atacttaaag ctattaattg gctactactt ctaagttttt taggagaaac ttcaaatatt
     4141 aatctttttg aagtaattaa aattaaagga gtagaaaaca cttgaaaaac acacagtata
     4201 ataatgatat ttgaatcatt tgttaaagca aataatagaa actgaactgt tatcataata
     4261 gcacttaatt ttaataagtt taaactgcta tattttttta ataaataaga accagccaga
     4321 tatgttggta tctctaacaa tgactgtaaa gaccacttta aagatatctg agaatttgta
     4381 gcatttaaag ctatcatttt atctattaca gtacagttat ttgctaccac cattgagtat
     4441 aataagaata aaatacatac tagtaagata tattttttat cttttaataa ttcaataata
     4501 tctttattag ttacatttat tttcttatta atcttttcaa tatcaggtaa taatagtata
     4561 tttataataa gtaatactac aattaaacca atcgctacag ataatccaaa atatgagaat
     4621 ctaagaatca agtaagatgc taccatactt ccaatagccc atccaatcga cccaaaagtc
     4681 ttaataaatg acaaataatt attaatatta tctccacaac ctattatcca tgtatcatat
     4741 aaactacagc aagtatttaa taatcctcct gataaagcaa caagtaataa gtatattata
     4801 aaagttgcag attcagtaga aaacataata actgatgcaa ttccataaac tgataaacta
     4861 attaaaacaa tttttttcat tgtttgatac ttatcagcta atactccaaa taataattga
     4921 aataatatat tggttattgc ataactcgat aatataattc ctctttcaaa cgctgagtaa
     4981 cctaattttg ataagaaagg tgtgaatttt gttgaagcaa aggcaaaaat aatatatgag
     5041 aaaaaataga atatgataaa tttaaattta atcgctttat taatcataat caatcaaaat
     5101 tgataacttt aataccacta tttccttcct gatttaagtt tgaaagatga tattttaccg
     5161 tattaggatt ctctacaata actaaaggaa tatataaagt attttcaagt aacttattat
     5221 caatttctac taacttttcc cccatattaa cttcttgacc ttgtttaact tctaatttaa
     5281 agttttcttt ttttgttgtt gcactgtcca atccaatatg aataagaatt tgaacatcat
     5341 tttgcaattt aattcctata gcatggttag tttgagcaat aaaacttatt actcctttag
     5401 tcggtgattt aagaatattg tcctttgcta tcatagcaaa tccatctccc attaccttag
     5461 ttgaaaatac tttatcagga atttctccaa gattaattat ttttccatca atgtatgaat
     5521 taattgtaat tgcttctttt tttcttttaa agaacattat agacactcct ctacataatt
     5581 aactttattc taattatcat tacaaataac gatattaagc atactgcaat tgaggaaaaa
     5641 agagccttat agctctttcc ccattgtagt tctttaatac tgcttatgat ttgtttaatt
     5701 ccaacaaatt tataaatata ggtaaacatg ataacaatag ttattgtgta aatttggaat
     5761 atctgcccta ataagactaa atagaaagca atcatgtttt cttttatctc tttattcttt
     5821 tttatattgt aaataacata tgcactgtat actgcactaa agttagagat taaatttaaa
     5881 cttgcattaa catcattgtt taaaagttgt gaaaatgaaa tatttgtaaa taatgatgtc
     5941 aattctaaat acactatata taaaacaggt aatattagaa aacaaaccat aaccactgaa
     6001 agaaatatat tttcatcttt taatactgct gtgatatttt ttattttatt tgttatcatg
     6061 tttttgttct ccttatttaa aagttggcca gaaatcctta ttagccacaa tcatttcatc
     6121 taagatttct tttgctactt ttgcacttgg aacagtttta ctcatagtaa acgactgcca
     6181 taatttttga taactttttt ctatccatgc ttctacagct aatttttcag aagcaacttg
     6241 ttgttccatt aaaccttttt ggaaagttgg aattttacct atagacatag gttcaaccat
     6301 atcttttcca acaaggcaag gaatttcaac cattgcatca tctgcaaaat tttcaatagc
     6361 tccattatta ggaacaatca gtaaaaatct ttcctgagta ttaaatgcta atgcacaagc
     6421 taattcaaca ataaattgag aatgctcact tgcatcaaac catgtatccc ttgctgtgcc
     6481 attttttata attctttcac attcttcgaa tgtatccttt tctcttctat ccataatttc
     6541 atttgctctt gtataatttg gattagcatg ttcaacagtt tcatcagcca ttaaataata
     6601 ttttaaataa gtattaggta atgtagttgg ttctatttct aataagtcct ttgctgctct
     6661 catagtttct aaccaactag attctttgtg ttgaccttga ggtgtaaatt cctcatatcc
     6721 ttttttagca caatattctt gtaatttagg cattaaatca tttccttctt tgtccttaat
     6781 acttgtccac cacccgaaat gattcaatcc ataatatcta acatcaaagt ctttacgaga
     6841 atttaaacct aaaactctag caaatatttc ctctaaacat actggcatat cacaaatatt
     6901 aataattctt gaatttggtc ttaatacacg acatgcttct gcaactatag ctgctggatt
     6961 tgaataatta agcatccaag catttggaga atatttttcc atataatcaa tcatttcgat
     7021 tattggacct attgttctta atccataggc aattccacct gccccacaag tttcttgacc
     7081 aacaacccca tattttagtg gaattttttc atcttgttca cgtaaagcat ataaaccaac
     7141 acgaatttgt gccattacaa aatcaatatc agtaaatgct tcttctggat ttgttgtata
     7201 actaaattta atttctggat ttttctctct aacaattatt gcacatgctt cagcaacttt
     7261 tctttgtctc tcatcattat tatcatataa tttaattgat tcaataggaa atttagcaac
     7321 actttcaatt aacattgtta caattccagc agtataagta ctacccccac cagcaattag
     7381 tatcctctgt ctttttcttt ccatctttcc ttctccttta taataaattt tcaaattctt
     7441 ctcttacact tggtactgaa agcccaataa taatctgaat agcttttcca ttatgcacta
     7501 aaccatgtgc acctactgcc ttaaaatctt cttctttaga aataattgag tcatctttca
     7561 aggttaatct caacctcgta gcacagtttg taacatctac aacattttct gctccaccaa
     7621 ctaattttat gtattcagat gcttgttgag aggctttact caatttattt cctttttcat
     7681 ctttagtttc taagtattca tttttagtat atagttttgt ttcttcacta tcttcttctc
     7741 taccaggagt tttcatatca aacttcttaa ttaagaaagt aaatacaaag aaccagataa
     7801 atgaaaatat aatccctaca acaacttgaa caacatatac cttccagtga tacattccaa
     7861 gaggaagcca atttaataca acccagttaa taattcctcc accaaaatca ccaacaactc
     7921 ctaaggcaaa cgaaatcgtt tgcaagcacg catctaaaaa tgcatgtact aggaataaaa
     7981 gtggtgctac aaataaaaat gtaaattcta acggctcagt aattccagtt aatactgcag
     8041 taagagtagc aggaatcatt aaacctaata cttttttctt tttttctggc tttgcagtta
     8101 catagaaagc accaaataca cctaatgaac caaacacctt cgataatccg gataatgaaa
     8161 atccgattgg atacatttcc tttagagtct tagctgaagt ttgaaaatcc tgaatatgag
     8221 tagcccaata tactgatgta ccacctggaa caacaacact atcataacaa attggtgcat
     8281 atacaaaatg atgtaatcca gttggtatta aaatcttttg taagaatgca taacaccaaa
     8341 ctcctatatt tccactattt ataaaaaacg tttgcaaatg tctaataaca ttttgaatat
     8401 gaggccaaat aaaacaagtt aataatgcaa ctggtatcat aacaaagaaa caaattgcaa
     8461 ctacaagtga tgagcctcta aataatccaa taaaatctgg tagttcttta tcataaaatc
     8521 tattatgaat ataaatgaca atacctgata ttaacaaagc accaaccata ccagtatcta
     8581 atgttttaat accagcaatg cttacaagtc cacttccagc acttatttca gctgcgaaat
     8641 caactccaaa gctttttccc caaaaactta acatagaacc tacaaaataa ttaaaagtca
     8701 aatacgttgc taaagcctcc atacaagctc ttgcttgttg cttttttgca agtgctattg
     8761 gtaaagaaat agcaaataat aatggtaatt gattaaatac catccaagct ccactagata
     8821 ttacacccca aaattggtac caaatatttt ctggtgtagc aagtgaaccc ataattgact
     8881 gattttgaaa tacaattgag aatccaacca ttatcccagc aaaagcaaat aatagtgttg
     8941 gtgtaaacat tgcccctcca aaacgctgta ttttcttcat catataaatt actcccctcc
     9001 tcatattttt agtttatctt tggccttaga tatctattat tttaacaggt taataaatat
     9061 atacaatctt tagggtacat ttggagctta ttcacagtca aataagtttt tgcacatttt
     9121 tatgttcaaa ttctcataaa aaaaaagaat gactacaatt tgcaatcaat cccttctgta
     9181 atcattctat gccttttata aatactatat ttaataaata ataactcaat caatataaaa
     9241 tataacattg tcaatttata actagggtgc atttgtaaaa atgagatatt agtcgctgaa
     9301 atataaattg attcatcact taaatcagtc aatgtattat ttttaaactc tgtaattgaa
     9361 attattttag ttcctattaa ttttaatctt ctagcaatat taagtgtatt ctcattctca
     9421 ccacttaggg atataataat cataacatca tttttactga caatattgta aaaagaatca
     9481 tcaaatgttc ttccctcaaa atcatatatt atttcttgac atgataagaa cattctcttt
     9541 atctgttttg caactacatt ttgaatatca cctgaaccat aagcgtatat tgttcttgat
     9601 tcataaagaa gtttacacaa tgcatcataa tctttatttt tttgatcttc aacatactta
     9661 attattgaat tacatgcaat atcaaatgac tgattttcta tgacatgaac cttttctgat
     9721 tcccagctta ataatacttt aaactcactg aaacctttta aatctaattt tttaacaaat
     9781 cttgtaatag ttgcacgaga cacattacat ttcatagcaa gttcatttat tgacatctta
     9841 ctacaatgtt ttttatttgc agttatatat ttccatatta ataaatctgt atcattcaat
     9901 ttattataat ttaaaaaaat catttcttct aatctcataa actattaccc cctttaatta
     9961 aaaatcgtca taaatattat accaatattc aacattaaat tacagcgtat tcttatattt
    10021 actatttagc ccataaaatt gactataaaa aatactgcta taattgatgt ttaaaaaaat
    10081 gtagttttaa ttttattctt ttatctaata attaatattt tcaagttcta gtgcttttct
    10141 tatacaatta tcatctttaa aatcagcagt ccttttacaa tcttttactt taaattctgg
    10201 aatagtttta aattcctcat cacatattcc attcttattt aaatacatac aacttgccat
    10261 tctaactatt agaccactat tttttaaatt aaacaataca gggtctatac accctccatc
    10321 tccgccagta gtttcaccaa ctaaagtcgc aaatttagtt tctttacaaa acattgcaaa
    10381 tgactctgat gatgaatata tttttctatc aacaagtaag tatatattac ccttaaattt
    10441 tgaatcatct gtagatttta tagtatagga agtatcttca aaatcattaa aatcttttat
    10501 aatttcttta ggtgcatttt cctttacact tgttggtaaa ttttctacag gatttaattt
    10561 tatatttctt gcatttgtat attgttttac tatttcatta ttatttctat aaattctata
    10621 accatttatt ttgacatcat tttttatgag tttagatacg attccctgcc aataactatc
    10681 acttccacct aaattacctc ttatatcaat aattaaagct ttatgttttt ctaaagtgtc
    10741 aatataatct ccaattattt ttaaatcatt atttatagag ccattctttg atgacattga
    10801 tggtaaatac atatatccaa tctgaccctt aataacatcc tttaaaatta attctttttt
    10861 tacaaaaata tcatctggca tttttatttt aagattcata gagtcatatc tatctacaac
    10921 ttttttatca ttaagaaaat cataccaatc attattagag tatgatttct taaaaagttc
    10981 atatcttttc ttattatcta ttacttctgt gtgtttatta tttaaatcag ataatataga
    11041 tgacatttct tctataaatt cttcatcatt ttcagtgttt tttattcgtt ttgtatattc
    11101 atccttattt gctaaccaat caatattatt aagcctctta tttacaccta gatatggata
    11161 tcctgtcttt acaacattat acacataatt aaagtcatcc accttttcct cttcagacaa
    11221 ctgattcttt tgagaataag tagaattacc taataagatt ataaatgcta aaataattac
    11281 taaaaataat actttctttt tcatgtaatt ctcatccact ttcagatttt atattttaat
    11341 acattgtatc attattttat tatcacctaa acagttaaaa gaaattcttg aagatatatt
    11401 ccactattat tctctttaaa aatttttatt tgtattttca aaattatttc atgaaagtta
    11461 taaaataaca attatattca aatatctatt ttactgctat atcctgaagg taaattctta
    11521 tcagtgatat actaaaaaag atatgctaaa aaatttatcc attataatga cttaaattta
    11581 gatactttac tgatttttat ttattagttg agaaagatat tgataattaa aaatattgat
    11641 aactatagaa atgattattt tttatagaaa ttttaaggat ataaactaat tatacttaag
    11701 tctatatcct taatttatca tgattatgag aatttttaca aatacttatc tttgtccgtt
    11761 ttagaataaa aacaagaaca tatcctgtta ttttattcaa acatccaaat ttaattacta
    11821 cacaattact tattagaaat tgtatctact tcatatatat atctgttaag tatagcttct
    11881 agcatttctt gtcttccaga cttattcttt ataactggca tttgcagtgc ataagattct
    11941 aactctttaa atccaacctt accttctact atatccttac ctataccttc actaaaactt
    12001 gcatatcttt ctttagtaaa gttttcaaat acctcatctt ctaataattt atgagctatt
    12061 aataatccct ttgcaaatgt atccattcct gcaatataag ctaggaataa gtcttctacc
    12121 tcaaaagatg ctcttcttac tttagagtca aagttaaatc ctcctggtgc tattccacct
    12181 tgctttaaca cttcatacat agcaagtgtt gcatcatata tatttgttgg aaattggtct
    12241 gtatcccaac ctaataatag gtctccttga tttgcatcta tactacctaa aacattgttt
    12301 attcttgcta tatttaattc atgttggaaa gtatgtcctg caagtgttgc atggtttgct
    12361 tctatattca ttttgaagta tttatccaga ttatactttc ttaaaaatcc taaaacagta
    12421 gcagtatcaa aatcgtattg atgtttagta ggctcttttg gcttaggctc tattaagaat
    12481 tggccagtga atcctatttc cttagcataa tctacagcca tttgtaagaa tcttgcaaag
    12541 ttatccattt caagttctgt attagtgtta agtaaagttt cataaccttc tcttccaccc
    12601 cagaatacaa aattttctcc tcctaagtac ttagttattt ccatagcttt tttaacctga
    12661 gctgctgcat atgcatatac atctgcatta catgttgtag atgcaccatg aacaaatctt
    12721 ggatttccaa acatattggc agttccccat aataacttta tacctgtctt ttccatatta
    12781 acttttatat actcaactat ttcatctaat atcttatttg tctcttctaa atctcttcct
    12841 tctggtgcta tatctctgtc atgaaaacag aaatactcaa ttcccatctt agacataaat
    12901 tcaaatcctg catcaacttt aatctttgct atctcaattg ggtctgttat attattccaa
    12961 ggtctctcta ctgtgcctac tccaaatggg tcattaccct ctccacataa agtatgccaa
    13021 tagctcattg caaatcttaa gtgttctttc atagtcttat tacctaccac ttgttcagga
    13081 ttgtagtatc taaatgctaa ttcattgtca gattttactc cttcaaattt tatctgtcct
    13141 attcctttaa atatttcact catttcatgt cctcctaata ttttacacat ataatatatg
    13201 tagtatgttt tcttaattta aaactatttt ctatctttat ttttcttttt aagctaattt
    13261 aattatttat ttttgcatta actacttcat ctaataactt aaagtcatct ttcaaagatt
    13321 tgtatattct tttatacaat ctatgatata tattgtatgt atctacattt tcacttattg
    13381 gttctaattt agttgaagtt tttattatag catcacatgc tttttgtata ttttcatata
    13441 ttccagctcc tactaatgca agaattgcaa ctccaagtgc tggtccttca cttgaattta
    13501 tagtatatac atcttgatta aacatatctg cttgcatctg tctccatact ttactttttc
    13561 cacctccacc tgaggctctt acttcattaa cgtctatatt aagattctct attatatcca
    13621 tacagtcttt aagactatat gaaacacctt ccattattga ccttgcaatt tcttttttag
    13681 aatggattgg agataatcca aagaacacac ctcttgcata agggtcaaga tgaggagttc
    13741 tttctcccat catatatggt aagtaaagca atccattaca tcctgttggt acttgactta
    13801 cttgattatt tataatctca tatacatctt cacctaaaga atttgaaact tccacttcac
    13861 tttgacagaa attatcttta aaccatttta atgacaagcc agctccttga gtaactccca
    13921 taacatgcca tgtatttggt attgcatgac agaaagtatg aactctacct tctttatcaa
    13981 tttttggctc ttttgtataa gcaaatacta cacctgatgt acctatagta gatgaaacta
    14041 ctccatcttt aactattcca tttcctatag ctcctgctgc ttggtctcca gctcctccta
    14101 ccactattgt tccttcttta agccctgtca aacttgctac atctctagtc acataacctg
    14161 taatctcttg tgattcataa actttaccta aaagattttt atctatattt aacttttcta
    14221 gtacttcatc gctccaatct ctttttctta tatcgagcaa ctgcatacca cttgcatctg
    14281 atacctctgt agcatattca cctgtcaatt tgtatcttat ataatctttt ggaagaagta
    14341 ttttattagt atttttatat atatctggtt cattgtttct aacccataat atctttgatg
    14401 ctgtaaatcc tgtaagtgct ggattagcag ttatttcaat cagtctttct tcacctacta
    14461 aatcagttat ctcctcacac tcttttactg ttcgttggtc acaccatatt atagagtttc
    14521 tcaatgtttt accttcattg tccagcataa ctagaccatg catctgtcct gataatccaa
    14581 ttccttttat acattcactg cttatatttg aactttgaac tacttctctt atagattcga
    14641 ctacagcttt ccaccaatca tttgcatcct gctctgccca acctgatttc tcttgtataa
    14701 gaggatattc atatgtacaa ctttttatag tatttcctag tttatcaaat aaaactgttt
    14761 tagtacctga tgtaccaata tccacaccta atacatactc caacttcatc cctccttgaa
    14821 tgccacttca taactatcgt tttcctatcc taattttttt atattttagg tatattttac
    14881 ttgctttatt tttgttagta tattaaacta acttatgaac taataataaa atatttaatt
    14941 atctttgtca atgcaaaata accttttatt tttgtgtaaa aagtattaca atattatttt
    15001 aaggaggaat atcaaattgg ttactgataa atacactatt cgagaaatga atgaaagatt
    15061 ggtacttgaa caaattataa aaaatggtcc tatttctagg gcaagtatag cttctactat
    15121 aggattgaat aaagcaacta tatccgctat taccaaaaaa cttattgatg aatctctagt
    15181 tcatgaaatc ggaattggaa atagtactca tagtggcgga agaaaaccca tattattagt
    15241 atttaataaa tgtgcaggga tttctttaag tatggacatt ggatacgact atatattttc
    15301 ttctctatct tacttagatg gaactattat aaattctaag aaattaacag atatacaagt
    15361 aagtaaagac aatgttatac aattgataga tgaaattata aatagttata acatatccaa
    15421 aatagacact ccttataagg taattggtct tactttggct atacatggga ttacttgtga
    15481 aaataaagta ttgtttactc catattacaa tcttaatgag attgacttat atagcatcct
    15541 tagcaaaaag tatgactttc ctattcatat agaaaatgaa gcaaacttaa cagctttagc
    15601 tgagaatact ttttcaactg tacataactc actacttagt ctcagtattc acagtggatt
    15661 tggttcaggc attataataa ataataaatt atatagtggc agaaatggta tgagtggaga
    15721 aattggtcac actatcatca tgcctaacgg taaactatgt ccttgtggaa atcgtggctg
    15781 tttagaacag tattgttcag aaaaaaaggt atttgaacag ctttcttctt tagaaaatat
    15841 tcctaaaata gattctgata tagtaaagca actatattat gaagataacc ataatgccaa
    15901 aaaagttatt catgagtttt gtagttactt aacaatagct ataaataatg ctattacaac
    15961 ttatgctcct gaaataatat atttaaatag ccaaataatt agtgatatac ctgagatatt
    16021 acagattaca aaagatatgt tagttagctc ttttaacaaa ggaataaata tagaaatttc
    16081 atcattaggg tctgaggcat ctctatatgg tggctctgct gtaaatatca agagcttttt
    16141 gaatatacaa aatttaactt taattaatga aatataaggg ttatgactat atacagccat
    16201 aaccctttaa attatacatt ctaaaattag atttattata tagtttatgc aagattagct
    16261 atatttttta aaaattgtat atcaattaag tattaacata agtacttcgt tttaaatatt
    16321 aatataatct tcctactcat catcagataa taaacttgca ttttgttcaa ccatagaaat
    16381 attatcttta cctattttct ctaattttgc tgccaattcc tttgtatcta aaaaagctga
    16441 ttcaataacc atacctaaat ttagccctga aataatttta attcgactgt cattattggc
    16501 tactaataaa gcagcattgc atggagttcc acctagtaaa tctgccatta taattatttc
    16561 tgaactttcc ttgtacactt ctaaagcttt ttttaatttt tctattgttc cttctattcc
    16621 atcatcttgt ttcatattta cagtaggata atctatagaa tcccctatta tcatttttgc
    16681 agaatttaag acttcttcag ccatattgcc atgactcatc aaaataatac tattcaattt
    16741 aatacacctc tttaaatcta taagatgtct acaagataag ttttcttatc atcaggcgtc
    16801 attatagttg taatttcaat tccttcatct aaaagtcttt taaatgcttc tttctcttct
    16861 tcagtaacac tgatattagg tcttagaact gttgtatctt ttctacttga catatttcct
    16921 acatttatat tatgtatttt aaatcctgcc tctacaaatg gaataaggtc cacaggagac
    16981 ttaacaatta ataatacatt ttgtccttca tactttcctg cagtaatatt ttttatagca
    17041 gtctcttctg taattataga tgacgctata cctgcaggtg tagccattct taaaacttgt
    17101 ttttgaacag aattgttagc tactgcatca tttactacca ttaatcttgt aacacccaat
    17161 ttattactcc acatagttgc aacttgacca tggattaaac ggtcatctat acgaatattt
    17221 ttaatacctt tcatttccat atctaatatc atcctttcct tattttaaaa tacctaatgc
    17281 atataaaatt attgaaacaa ccattactcc taaaattaag tatataggtt ttatattctt
    17341 tttaagagcc caataaacaa tcacaactag aattgcagga actaacccag gcataatttg
    17401 gtctaatatt tcttgacctt tcattgtaaa atcaccttgt tgaaagttca aagcaacatt
    17461 agcttttaca actgaaggta ttaatgcacc tacaaccatt aaacctaaaa tagatgtact
    17521 ctcagttaaa atctttaaac ttttttcaat tgaagatatt agcttagtac ctgatgtata
    17581 accagcatta aatagtggta tacttaataa tttcaatgct acactcacta caatccacag
    17641 taagattcca aatggacttc catctatcgc catattagct gctatagctc caaaaattgt
    17701 ccacggaata acgacaaata aactatctcc taaacctgct actggtccca tcaagcctgt
    17761 tttaattgaa gcaacagcct cttcagtctc ttcacttgat aactcctcca ttgctacatt
    17821 aatacccaaa atataagggg caacccaagg attagtgtta aaaaattgta aatgatttaa
    17881 tacagcactt tgtaactttt ctggattatc tttgtatact acttttaaaa aaggtagtac
    17941 tgcataacag tatgccaagg cctgcatctt ctcataattt gaagaaacag aactagtaaa
    18001 aaaccagcgc cataagacac tttttttcac cttttcattt gatttattta atgatacctt
    18061 atcactcatc ttcgtccata cctcctacta cagtatattg ttgttcttga ttttccaaat
    18121 tctttttgta aattataagt gcaattgctc caccaattaa agctacacct aataatggta
    18181 ctttcatata tactgctaaa acaaaaccaa ttactaagta actaaaatac tccttagcag
    18241 gaagatatct aagtaataat cctatcccta ctgctggaag tagtcctcca gctactgtta
    18301 aaccacctgt taaccattca ggtgtatatt tcaaaatggt attaattaaa gaaggtccaa
    18361 atattacaga tagagcaact ggaatacctg ttgtagctgc tgttaatagt actcctaaca
    18421 tttgcatata ggttatattt ttataatttc cttttttagc tccatcttct gctttatgaa
    18481 ctagccatat accaatagta tttctaagaa catctaattg taccatcaat aaagcaactg
    18541 gaatacctat agagattcct acagatgctt cttgcttagt cgtaatagtt ataaatgtgg
    18601 ctactatact tgctgtttga taatcaggta ttgatgctcc accaaagtta ctaataccta
    18661 atgataatag ctgtaatgta cctccaatat ataaccctgt atttatatct cctaagataa
    18721 gaccagtaat aaaacctgct gttactggtt gatatgttcc aaacattgtt gaattttttt
    18781 catagtttat aaaaaatcca tatagtacga ttaataataa ttgccataat tgtggctcca
    18841 ttatgatttc acctctccca aaatattttt tagttcttta aaattataac tggaatcttg
    18901 ctttaggaaa ataggaattt cttccagtgg gcattcaact tcataagttt tcccaccttc
    18961 ataactaact ccactaaata tattttccca tgtatgtcct tttggtaaat atacttttct
    19021 acttctatct ttataattca ttattggagc taccaaaatt tcatctccaa gcatatatgt
    19081 attatccacc tcccaagttt tatcatcttc aggaaattca taaaataaag ttctcataac
    19141 tggtgttcca tcttcatgcg cttctttcat cagttttttt aaataagttt ttaagctctc
    19201 tctaattttt atgaattttg taagaattac ttcaacttct tcaccataag accatatttc
    19261 attaggagct ccagttacca ttgagcctcc acctttattt gataatggtt tactatgagg
    19321 caatctatct ccatgcattc tcaaaattgg tgaaaaagta gcatattgga accatcttac
    19381 cataagttct ctaaattctg ggtctttagg atttcctcca tggaaacctc caatatcagt
    19441 agtccaccat ggtatacctg ccagtcccat atttaaccct gtatttactt gattattaaa
    19501 agcttcaaaa ctagaatcaa tgtcaccaga ccatactaaa gctccatact tttgagctcc
    19561 tgcccaagct cctcttacaa gagttacaac agactctatg tcattatgta aaccatcata
    19621 aatcattttt aaataatcaa taggataaat attaccacaa cttaaaacat ttcctttttt
    19681 atatctatag ttatcaaaat catacacaga atagccaggt tcagctacat ctaaccaata
    19741 atagtcaatc ccaagttgtt tataattttt atctattcta tcccaaacat attctcttgc
    19801 attttcattt gtcatatcta caaagactgt atttccttga atttgcattg tcattctaac
    19861 accacgatta acattgacta aatagccatt ttctaaataa tcattgtagt tttctgcatc
    19921 actttgaact gtaggccata cagatactat tggttctact gaatactctt ctttcaattt
    19981 tttaactaat tctttaggat ttttccaata atctaagtca aatttatatt caccttgctt
    20041 aggccaatga aaatagtcaa ttgcaattga tgaaagctta attcctcttt tagaatattc
    20101 cttcacaaca tctaaaactt cttctgaagt tctatatctt agtttacttt gccatagtcc
    20161 taaaaggttt tcaggcatca taggaacagt tccagtaact tgagaataat ttttatttaa
    20221 ctcttttggg ctttctccac aagtaatcca ataatctata aaatttgtag aaaacatctt
    20281 ccattctgtc atatttttag caaaagatac ttttccaatt cccggattat tccataaaag
    20341 tccataacct agactagaaa tataaaaagg aactgatatt tgagaatttc tttgggctaa
    20401 ctccaaaaca gtattcttta aatctaaaaa tttatgttga tattgtccca ttccaaaaat
    20461 cttttcagaa ggctcagatt caaatctagt agttacttca aattctccac tatagcttgg
    20521 tcgatattct cttgatttta actttagagt agagttaaag tctttggtaa tctcaattgt
    20581 ccctacatct tctccaccat catcatgttt tacagctctt aatcgaatat attctttcaa
    20641 taatatttca tttttatcat tataaaaagt aattctatct ctatggtcta agacagcttt
    20701 tatctttcca tttttaatga ttgcacttcc atcttcattt ttattaattg taatatcatc
    20761 tttatttaac ttaggttttt cgttaagagc atagttttca tcaacaaaat tttggtctac
    20821 aaacgaccta atacgtaaag agttttttcc ccatgcttct attcgcaata gttcattgtc
    20881 aaatcttttt tcaatatagt cttcagatat ttttatcacg gctacctcct aaatcaatgg
    20941 tataatattc tatctttgtg tagcttaagt taaataatgt cgtttagtat cgttatttat
    21001 aaattaggtt ttatatataa ttcatttgta cattaaacta acttaccaat aatatacttt
    21061 atttgacttt gtttgtcaat cgctttccgg attttagtaa aattttctaa tatattgctg
    21121 gaaagtcgtt cttaatatct tttatttata cttttaatat ctgtgatgat acctatatac
    21181 atctagtttt tttaaaacac atacaggaat aacatttgcc tattatgtag ataatatcgg
    21241 acttgaaaat atgtatagtg accttataaa tacagataat aatatattag atattgttca
    21301 aaacaatgga ttttataatt ataaataatt tattaaatta tttaagaaaa tatacaattg
    21361 tataccaagt caaaaacgac aacaaataaa taaaactaaa aaagatgttg tctcaaaata
    21421 tagataaatt tatttttcat tgctaaaatg attaaaatat atttgattta atatacttga
    21481 tttaatataa aaaaagagtg tctcataaac taaaaagttc acttttagac actcctttat
    21541 atatattatc tacttcttaa ttcatttatt acctctctac attttctttc aactagtgga
    21601 tacatcttta taagtactaa ttactccact taaaaatgat tcaggtactg tccatcagtc
    21661 atatttttct gccaaatata atggtgtttc ataatgagac aataaatatt tatatctcta
    21721 cttatcttct ctaagctcgt caaatccaac tataggtata tttaaattat attctttaag
    21781 tagcttttct agctcttcta taaaaatacc tttcccatcc accatttgta tatcatttat
    21841 tttatccatc aattcattgc aaccacttaa ctcaccaaac caacgtttag aactacatgt
    21901 cttattataa ccacaacttg gactgccatc tattgcaatg atacttttta tatcataatc
    21961 attttttaaa tattcatcaa actgccttac atgaggtatt aacatttgcc tacattgttc
    22021 tctaaaaaac aaattgtcaa actgttcttt tacatgtccc cacctattta ttccatacat
    22081 aatcatttct gggcatggta gttgaattat tccataccct ttatttatta aatctatcgc
    22141 tatattttgt gctcccttaa attctgacaa tccctctact ttagaattac aatttaatat
    22201 acaatgacat actactacaa tctctctatt tctcttcata ataatctcct tagttattct
    22261 taattttaat gatactacat attttgataa ttatattatt tatttgttct atatttccaa
    22321 tattctatat agaaatatac aattagtaat cttttataat ctatactatg ataaatgtat
    22381 atattttttt aggaggtatt ataattggat actaaaaaag tcacattttc ttcaatgtgc
    22441 atagttatta atattgtctt aggtatggtt atatctatga taaatatacc attattattt
    22501 ttagatacaa ttggtactgt acttggggct gtcgtattag gtcctttttg gagtgctctt
    22561 attggtgggt gtacaaactt agttttaggt gttatatcag gtcctacaaa tatacctttt
    22621 gcccttgtaa atattgttct tggtcttatt gttggatata tttctaagaa aaaaggtttt
    22681 ggttataaag aagctattat tacaggagta attttatcta ttgtttgtcc tttaattggt
    22741 actcctattt cagttttatt gtttggtggt ttaagtggaa gtggtgctga tttattggtt
    22801 ggttttttag ttcagtctgg tcagaagata tttactgctg cgttctttcc aaggattctt
    22861 agtaatatag tagacaaacc tttgtcttgt attatggtgg tatattttat aagtaaaatg
    22921 cctaaaggtt ttataaaaca atatagtaaa aatacttcta gttcatatta ataaatcatt
    22981 tattatgtaa gatttattac tctttaaaat gctatatact catttttata taacattttt
    23041 attaataatt taattagata gtatttgtat tattttaatt aaacgtaacc tcttgatata
    23101 aatttgagct ataaatctat atcaagaggt gctattttat ttttaatata attaatattc
    23161 tattacgcaa aaaatacata tatttcatta ttttatggaa aatattactt catttaactc
    23221 tatatcaaac tcattaagta aaaatctaat tatatttaat ttagcctctg tatttaaatt
    23281 tgtttctaaa tatatatttt ctgatatttt aatacctttt cttaatttat atgcatctct
    23341 agacttaata ttatctataa aatctctttt ataagtcttg tctacaatac tgttaaacct
    23401 tacagaatcc aaatcatata aagtaagagc aacttgttca taaaattctg tccaattttt
    23461 agccttatat gactctccta atatactaaa tgatactata ctttcatttg taaaattaac
    23521 atcatcatcc tctaacgtaa atatattttc actgttactt ggaggttcat atgtaacact
    23581 tggatatttc caaatttcta cagctctatc tagtaatatc ttagttctat tttttatttc
    23641 ttcttcattc catttatcta tagatgaaat atatttattt agataaagcc tactatcttt
    23701 aaatccatct tccatttctt tcttttcttc aaatgtttta ttacttagtt ttgaattata
    23761 tcctgtcaaa gttaaatttc caattgtatg taaatattta tcatgtatct ccttatagtt
    23821 atctcctagt gattccttcc atttatttgt taatgtctgt ggcattatat gctctatatt
    23881 tagcatatta ttattgatta aattttctaa atcaacaatt tctttattat tataattctc
    23941 taacctctca agtatatata acttattctt tgacttagta ttatatatat ctttagtttc
    24001 aaaagcattt gaaaattcac tgtcagtagg aaatctttga gatgatttct tatttataaa
    24061 aatatatctt aatatattag aatagttttc tttataatca ctaaattttt taatttccct
    24121 tcctaagttc ataaatactt tattcagtgc attaggaggt acatcacata ctattcttct
    24181 taatataaaa cattctacta aattaaggga ttctacaagt acttttttat ttattatacc
    24241 ttcattataa tcatcaaata attctaatat aaacggatat gatacaaata tttcaagttt
    24301 gtttatcctt ttcaaaactt tgtttacatc attatcatca gtacatgaat aagatatctc
    24361 tctataatag ttagaaaatt taagtaaatc ttttaataaa atttctatat ctataccatt
    24421 atctgataca taccttttaa aactaaaata tactttattt ttattcggta tacttctctc
    24481 ctttatagtc aaatagtctc ttataaaact acttacgttg ctacttgtat ttttttcaat
    24541 tttataccaa taattattat aaaattcctc ttgtaactta gagcttctac ccattaacac
    24601 aaagtttctt actctatcag cttcacttaa atctaagcca gttgaattta gactttcaaa
    24661 gattaattga gggtcatctt caccattttt taactctatt tctactatag ttaatttctg
    24721 tatagcatca aacaaatcat ctattgatat attatcttct tttattctat tataaaaata
    24781 taaataattt gaaactatat tagaatttct atctgtattc atttcttctt caaatagttc
    24841 atagtacgct tttctatctt ctgctattgg ttttaacttt atttttccat ctttatctct
    24901 tgagtgcttg tttataagat aacactcctt aatctgttct tctgttgtaa taattccttt
    24961 tgattcccca aactctttta ttatttgaca catggcaatc aataatattg acacagtagt
    25021 cactctttgt tgtccatcta ttattaaata ttcttttccc atactatata tagatacaac
    25081 tgaccctaag aaatgagttc taaatccaga atctattata tcctctaaat catcaaacaa
    25141 tcttttacat tgttctttct tccagttata atttctttga tatacaggta ctacaaaact
    25201 tttgtttgac ccctctaaat aactaagaaa ttcaatttct cctgctttca tattaactcc
    25261 ccctataagt taaataaatt ccattcataa ctccatgtat ctatatatta tatcaaaatt
    25321 ttgactcata aatgtatatg aaagccttat tatatacctt tttatacctg tttttatttt
    25381 attaaagtaa atacatgatt attctataaa atatacaaat acttaatttt aaaaattatt
    25441 atttaatctt cttcatgagt aattcatagt tatttatcaa ttaaaaagag atattgcata
    25501 attattcaat acctctcttt gtatatttac attatccata tctagttatc tatttcaacc
    25561 caatattaga ctacctacta ataaatttat tctctacatc attaacaata attttgcact
    25621 aaataacatc attagcctat ctctgatgca ttttctttaa ttttatttat agttttaatc
    25681 aatttatttc tctcttcaat atgattttca aataaatcaa ttatacttcc aacagttcta
    25741 aatgggtctt ccatcaacac tttgttatcc tctataaatc cattcttgac tacataatcg
    25801 actataagtt taacaaaatg tatttgctta gtattaaagc tcttattatt taaaaactct
    25861 gaaaaagctt catttgcagc ttctctatcc aatcccacta gtcttctcac taattggctt
    25921 actggagtat ctccaaactc cttcttataa tcatcacaat tacctaactg ttttaagagt
    25981 atttcctcca acgtttcaac atcttgcttt gttatctttt tattatttct taatttaaat
    26041 attgctaatt catctttatg gctatttaaa taatactcta ccttcttttt ataattctta
    26101 agattgtttg cattatatac agaatcattt ctatcttctg ctacaatcat atcttcaaaa
    26161 cttgtataat atatcttttg actagacttt tcaagatact taataagctc tcttaatgat
    26221 atacgtactt cttctacctc aaatatatct gaatcttccc aaaaatcctc tgtcattgct
    26281 ttttcaatag tatatttttg ttgctgaatc tgtggtatag tcccaagttt tgaaagtttt
    26341 tctgctgtag ccattacatg ccttatacct ttacttgcat tattattttg taagtatgca
    26401 agttgtatat tatacataac tacatcaaat cttttagcca attcatcatc actatatgaa
    26461 gttatgagtg gtgatatata ttctcttatc tcattttgtg ctactgctcc aatattgctc
    26521 caaacatttt catttttata cttatgtaca taacttaaat tcatcctaac tatataacta
    26581 tcctcattta aagcttttac agaagttata agttcttcta acaattcttt tctaagttct
    26641 gcatactcac cttctatata tttaatatct tgaagttctt taaccatact tacctttata
    26701 ttaaataatt tttcagtaag tgtttctgtt ttatttccct caaatccttt aggattaaat
    26761 ttaaaaaatt caaaattatt acaaaaatca aatattaaga atctttcctt atccatatca
    26821 acacctaata aatctggaca taatctagtt cctctaccta tcatttgcca aaacttagtc
    26881 ttacttctca ctttcttgaa aaaaacaaga tttagtattt caggtatgtc aattcctgta
    26941 tcaagcatat ctactgaaac tgctatttga ggtaatttat tcttatcaga aaaatcatct
    27001 attatagaat ctacataatt tatactatag tcaactgctt ttacaaagtt cccaccatac
    27061 tttggataca gtttattaaa tctttcaact attgccctag catgctttgt attctttgca
    27121 aaaattatag tcttaccaat cttttcttct ccttctattt tcaacccctc tgtcattagc
    27181 ttatttaaaa ctaaatctat tgtgtcagaa ttaaatagcc actcatttac tgcattattc
    27241 cctatttcgt ctccaatact ctcatcatca ttaaatgtat tttcatattc ttccttctct
    27301 tcatcagaaa gctcatcata cttaattcca tcttccataa tcttagtctt aaactcaata
    27361 ctagtataat ctactaaata tccatcctcg actgccttat tatactcata agcatatgta
    27421 ggtactccat tttccatgtc aaaaactgaa taagtatttt tatctatctc atcctttggt
    27481 gtagcagtaa gacccattaa gtaagaatca aaataatcaa agattgactt atacttttta
    27541 taaatacttc tatgcgattc atctattata attaaatcaa aatgaccagg tgtaaacaat
    27601 ctctttccat ccttagactt cgtatcatca attgcattca tcattgttgg atatgtagaa
    27661 aatatcatcc tagcatcttc tgggctgtct ttgctatcca ataaattaca taacgccaag
    27721 ttaggtaata gcttagtaaa acttttttta gcttgcttta ccaatgcttt tctatctgct
    27781 agaaataata tatttttaat ccaattatgt cttgaaagta catcaactat agatatagca
    27841 attcttgttt ttcctgtacc agtagcacat accaaaagag ttttgcgttg cttattttca
    27901 agagcctcac atacagccct aatagcttct ttttgataat atctattgga tatttcatca
    27961 tttattgtta cagaacttaa agacttctta ctctttcttc tatctatcat aagttgtaat
    28021 tctgattttt tataaaatcc ataaactttc ctatctgaat agtcatccca aatatacata
    28081 tcaaaaccat tagtcaaaaa tattaccggt ctttgactat atctcttttc caaacaatct
    28141 gcatataatt tagcttgttg ttgaccaatt ttcgggtctt tactcgttct tttcgcctca
    28201 actactgcta atggcttacc attttcacca taaagaacat aatctacata tcccacacca
    28261 ctttcattag gcataccttg tacttctatt tcttcccata tatcttttcc aaattcccaa
    28321 cctgcaagct ttaattctat atttatatat ctttttcttg tctcaaattc gcttacttcg
    28381 tctatcttga aatcatagtt tacagtatta acttttctct tttcagttag tgactttctt
    28441 aattcctcat tttcttttat tacttcttca agctttttat cttttgaact taatctctca
    28501 taaagatttt taagttcgtc tggtctcgtc cttttctctt ccccatgaag taataaatct
    28561 tcattaaagt cttctgctgt atattcatct gaataacaat agtctatcca actaacaaat
    28621 tgatgcaaat tgttaagtga aagtattgct tctcctctct ctatgttcgc actagtatgt
    28681 acagataaat tacctaattt aactatatat tttaataaag gtaacatatc atagtctata
    28741 agttctataa aactattatt atgaatcaac gtactaagat tatcttgata aggcaatact
    28801 aaatcactat cattagcata tagccatttt actgcaagct ctaaagctct tcttgttata
    28861 gttgcacaag tagatgggct aactagtata cttttttctg cttctaagca agcagtatta
    28921 aaactattga aatttttctt cttttgtaaa aaatcaaaat tacccaaggc ttttatcccc
    28981 cttttgtccc ttagttaaaa taaatctctc taaaaactat ttcttacagc tgtttcttat
    29041 actaacaaat acttcttcat tttctgtaaa caattcgtac atatcttcaa actgtggtat
    29101 atcatatcca tgagaatcat catttctcaa ttttctaagt gtttctataa ctatagaaga
    29161 atttttaggt aataaattgt gatactcaca agctaaaagg tagtcataaa agtttttaga
    29221 ttttatgttt aaatcaattg atttataata caacattgct tttgttaatt ctagaaatga
    29281 taccatatag cttttaaagc cttcttttat gtatctagcc cataactcag gcatttcttc
    29341 cttatcatat aagattttga agttttgcag gtcatacaag caagaaccaa aatccctatt
    29401 tacttcttct attgagttcc aagataattt gtttctaatt accatacatg tccctccaat
    29461 ctaagatatc tattacatca ctgttttcaa aatagaattt atttatataa tccttaaaat
    29521 ctttagattt ataaataact tcaccttcta aaatagcttt tttaagtgct attgtataat
    29581 tatctgtaac aatttttagg tctacatcaa tgtctaatat atttcttaga gcttctgtct
    29641 tagttgcaag ttctgtaatt ggtacttttt tgatgggaat ccaagctatg tctatatcac
    29701 tttctgatgt atgtttatct gttccataac taccaaatat tatgattgct agagtatttt
    29761 cttttggaaa tacatttatt attgagtttt taatctcatt tggtatattt ctcatttaat
    29821 cacctcttgt ttattaggta atattgctta attgaattta tatccataat cttttaatct
    29881 atttatacat atttctcttt cttcatcagt aaacaactct ttatactttt cttttaatac
    29941 taatacttct aatgataaat ctaatctctt acactcccat agccttgtaa atccttcagt
    30001 agctccttct ttattgatta attctttagc agctaataag gctcctttgt tatttatcag
    30061 ttgtaaaaat cttgttggct tatagttaca ttgtaaatca gcttctttat agatatttac
    30121 catgttatgg tataaatcta gctctatttg attcatagat tttattcccc cattttaata
    30181 attgtatttt agttgaataa ctatccttta aaagcttttt gcattaatga gttaaagttg
    30241 tcttctagtt cttttagact tgtttttatt cctactttca atttgtctac ttgattaaca
    30301 aactcttcaa acttattttg aagttctatt gatggtactg gtattaatat tttttgaagt
    30361 tctttgcttc taattccttg tgcagagcta ccagttttat atttatctat ttctacccta
    30421 aaagcttttg tttgcatata ttgtgctaaa aaagcattat ttaaaatttt tttatctccc
    30481 tgaattgtta taatcctttg acccatagca aactttttta aatcctttgg tatcctacat
    30541 acatttccaa atgtatgacc ttctgtaaca aaaagtacat ctccagctac tggaaaacct
    30601 cttttcatcc aagaattata ggtgttttca gatatatatt cattaatata tttaaataca
    30661 ccattcccta cagattttgc atttattatt ctaattccat tctctgaaag aattggagtt
    30721 ttaccacgat aatcaataaa atttgtacaa tctttcaata atacaaactt ttgttcctta
    30781 aaatttccaa acatttcaat aaatcttgat ttaactaatt catccaatgc ttctatttgc
    30841 tcttttcttt tatctattaa ctcttgagct ttatctaaaa cttttgatat atctttttga
    30901 gcttccattt caggtaaatc tacttttaaa ttttttaaat cattaactga aaaacttgct
    30961 tgattaactg atttttttat tatccttggt aactgtcttc taaattcatt actattaaaa
    31021 aaataattta aatactttgg ataacaaact tctttatctg gtcgtaaaca taaaagattc
    31081 attccatgta taattttttt atttatatct tcatatattg cacttttccc taaatgtttt
    31141 tcactattaa tatgtgacat tagtatatct ccatttttca ataaatagtc ttctaatttt
    31201 cctaactctt ttatgtcagc atatcccatt ttattagtat caatataaga gtttgctata
    31261 gtttctattc ttgtaattgg atatccaaat tttgtatcaa attgtttaat actagcccca
    31321 tttctaataa attcaaaaac ttcattcaat ctatattcca tactctacac ctcaagcatt
    31381 ctttctaatt cctctaatcc atctacgatt tccttctcta actccctaac tctacctata
    31441 ataacactag gagcatcata aactacttcc tcatacacta cctctttata cttattaata
    31501 gataaatcat acccattctc tcttatctct tccacatcaa caaaaaagct ttgctctgtt
    31561 ctttttctat ctacttcacc atcaagatta tcaaatctct taataatatc ctctatatca
    31621 ctatccttta tctcatttct cttatcatcc aaagaataac catcagcttt catatcatag
    31681 aaccatacct tatcagttcc acctgttcct gttttagtaa atatcattat agctgtagat
    31741 actcctgcat aaggcttaaa cactccactt ggcattgata ttatagcttc caatttatga
    31801 ttatcaacta tttcttttct tatcgattta tgtccacctg ttgaaccaaa caatactcca
    31861 tctggcacta tacatgcgca tcttccacca atttttaaaa ctcttaaaaa aagtgccaag
    31921 aataataact ctgttttctt tgtcttagtc acttttaata aatctgctga cactgcttca
    31981 taatctaaac ttcccttaaa aggaggattt gctaacacca atgtaaactt atctttatct
    32041 gtattaacct ccgataaaga atccttatac tctatattag gattgtcaac gccatgcaac
    32101 atcatattca tagcacctat tctaagcata gttctatcca tatcaaatcc atagaacata
    32161 ccattattaa aatgctcctt cagcccttgc actaagaaaa gacttgaatg attatccctt
    32221 aagtattgtt gtgatgatac taaaaaacct gccgaacctg ctgccgggtc tactataata
    32281 tcttctggtg ttggtttaat aagtcttact atcatatcta ttatgtgtct tggagtccta
    32341 aattgaccat tagtacctgc tgtagccact ttggataata aatactcata caaatctccc
    32401 tttgtatctc tatccttact taattctaaa ttactaatac catcaactat tttagataac
    32461 ataagaggcg taggaatttt aaatattgca tctcccatgt actttgcata agctgattct
    32521 ttatttccat gtaatttttt tatgaaaggg aacacttcat tttgtactat ttcatacatt
    32581 ttttctgaat ttccttcatt agaaaactta ctccacctta aatgttgatg ttcatcatca
    32641 aatatctttt caaaagaaac acccaaaaga gttgcttctg cttcattttt tgtttctact
    32701 tcatccaatc ctttaataaa taaaagatat gtaaattgtt ctataacttc taatggatta
    32761 gttaatccac cagtccaaaa agtctcccaa atcttgtcca ctttactcct taattctcct
    32821 gtaatcatat atgactttcc ctccagtgtt cataaaataa tttaaatata tattttagtc
    32881 gtacgcctta atagttagtg tcttatgtta aatccaagtt tttactacac aattattaaa
    32941 ttatttttcc tatttacata ttttagctaa aaaactaatg caatactctt attttatgat
    33001 tattatatat accatacaaa attattttat catatatcca tcatagaagt catctacctg
    33061 gataactctt accatattat aactttttaa tattaattta aacttagaaa gaaatttttt
    33121 atttaattat atcatcttcc ttatttcaaa tttctcaatt acaatcttaa tcaaaaaata
    33181 aaataagatg ctgtctcaaa atagatataa tcttcttttt tattgtcgga atgatgaaaa
    33241 tatatttaat ttcatacaaa aaagagtgcc tcatgaacta aaagttcatt ttgagacacc
    33301 ccttaattta atcttactta ttctttccct ttattattaa cacttcacta tctctattgc
    33361 ttttcctaca catccacaaa cttccatcct actcttaaca aaatctttaa ctgactctaa
    33421 acctttttta ccatattttt tagggtcaat tacctctggg tcagaattaa gcacatcctt
    33481 aaccccattg ctataagcaa tccttaattc agttgcataa ttaaccttgc aaatccctct
    33541 ttttatagac tccttaataa cctcatctgg tattccagac ccaccatgta atactaatgg
    33601 aactgataca acctctctaa tttcagataa tctatctaaa tcaagttttg gctctccctt
    33661 atatagccca tgtgccgtac ctattgctac tgctaaagat gatacccctg ttctctctac
    33721 aaactctttt gcttctaatg ggtctgtata tgctccacca tcaccaccat ctaaatcgtc
    33781 ttccttacct ccaactttac ctaattcagc ttcaacaggt atatctgaag gcatacatgc
    33841 atctacaaca gatttagata ttgaaatatt atcttcataa ctttcatgag aaccatcaat
    33901 cataatagaa gtatatccaa ctctaagtgc ctgcatagct agtccaaagc ttgaaccatg
    33961 gtctaagtgc attgctactg gaacacttgc cttctctgat gctaccttta cattcgctaa
    34021 ataataatct agtcctgcat actttattgt tgaaggtgtt gtttgcaata ttactggtga
    34081 tttaagttct tcagcagcct ctattactgc cataaccatc tccatatttt caacattaaa
    34141 tgcacctact gcataatgcc cttcttgtgc atctaataat atttgtttag ttgtcactaa
    34201 tgccataatt tctcctccta tatgtttata ctgatttaat ttcttcgtta tttaattttg
    34261 atactatttc tctacttgtt ttgctattta ataactcatt taaaaattct tcattcatta
    34321 attttgaaga tagttgagat aatgttttta agtgtaaatt atcaccatta tctgtagctg
    34381 ctatcataaa tataatatgt gccaagcttc catcttctga ttcaaagtca aacccttctt
    34441 ttgatagacc tactgctaca cgtggtacct ttactgcatc tgtttttgca tgtggtatcg
    34501 ctatccccat accaaaacct gtagatgata tttcttctct tttaaggatt tcttctttaa
    34561 acttcttttt atctaaaaga acactagatt tatcaagtat tccaatcatt tcatcaatta
    34621 catctgtttt attttttccc ttaacatcta aaattactgt ttcttcgcta tataaagaga
    34681 tattattaga tacgttttcc gattctgcac tttcattttt atgttcatct tttgttacat
    34741 ttgtattttc tatagtattt ttctcattta ctactttagg tgtaaccatc atataaagta
    34801 cagcacctac taatgaacct acaaatatac accctaccca taaaagtggc ttattgacta
    34861 atcctagaac caaaaaccct ccatgtggag ctggtacttc tattttcatt atataagtta
    34921 aaattgatga tattgatgat gcaatcataa gtactggcaa cacttttaat ggattttttg
    34981 ctgcaaaagg tattgccccc tctgttatat gagtactacc tagtatataa ttgattaatc
    35041 ctgctgctct atcttcttct gtaaatctat ttttaaataa agtagatgct attgctatta
    35101 ccaatggtgg tgttatacaa gctgcagata cacctgccat aaataaataa tttccctgtc
    35161 ctagaagcat agttccagtt acatatgctg ctttatttac tggacctccc atatcaaatg
    35221 ctgacataca tcctattact attcccaaaa ttatagggct tgaattttgt aaccccgata
    35281 gaaagttcat catagcattg ttaagtgctg aacatggccc accaagtaac aacattgcta
    35341 cacccacaag acctacgctg acaataggaa ttataaaaat cgatttaaga ccttctaatt
    35401 gtcgtggtaa ttttcttaac aaatgtacaa atagtttaac taagtaacct gctaaaaatc
    35461 ctgatatgat tccacctaaa aaccccgaac ctgtatcaaa tgatatcatt cctccaacaa
    35521 gtcctgccaa cataccagct cttcccgata tagattgagc tataaatgct gccaatacag
    35581 gaaccatcaa gttcatagaa tatccaccaa cagattttaa tgtagctgct atttgattat
    35641 attgttctga atttgggtca aatgaataaa tcccccataa aaatgatata gcagtaagta
    35701 ctccacctgc tactactaaa ggtaacatgt gagatacacc attcattaag tctttataca
    35761 acttttgacc aaacgataaa tttccttctt ctgcaatttt actgcttgat aagtttcctt
    35821 ttctaatagc tacttttcca tcaatacatc tttgtataag ttcattagct ttatgtatac
    35881 cttctttaac aggtacttct attactggct ttccattgaa tctgtccata tcaacatttt
    35941 tatcacaagc aactattatt gcatctgcca tttcgatatc ttttgctgtt aacttatttt
    36001 ctacacctat agctccattc gtttcaactt ttatgtcaca ccctaattcc ctggctgaat
    36061 tttttaatgc ttcttctgcc ataaaagtat gagctattcc agttggacaa cctgttaccc
    36121 ctagaatcat tttctttccc atttttatac cacctttctg actcttatat tttttatata
    36181 atcttccaca ttctccaaat tacctatacc attactttca gccacacttg ctcctgttgc
    36241 tgctgacctt tttaatgctt cttcaatatg ttctggattt tccaaccata tacttaaaaa
    36301 agcagctaat gcagaatcac cagcacatgc agagctgact actgtaactg gttgagcact
    36361 agcataataa attgattttc cattataaaa ataagaacct ttttctccta aagttagcaa
    36421 tatattttga gcacctttta aatgtagtaa tttaagtaca tccttaatat cttcttcatc
    36481 tcttacaata attccaaata tatctttgat ttcttcatca tttggtttta tcaaatatgg
    36541 attatactgt agtaactcct ttaattttgg tgaacttata tcgagtattg tctttatact
    36601 tttattttca caaattttaa atatatcctc atagtaatta tcatcaatac caggaggtag
    36661 acttccactt attgataagt aacttatatc ttctaatgac tgtatcttat ctagcatatc
    36721 tagtttttgt ttttcgttta catatgaccc agagtttaca aatttaaatt catcacttcc
    36781 atcatttaaa aatatattta tacgagttgt atcttctacc caagtaggca atacgtcaaa
    36841 tcctctcttt tcgctttctt caactatata ttttcctgaa aatcctccaa aaaatcctaa
    36901 tatcttagat tctataccat aatgacctag tacaaaagtt acatttaatc cctttccatt
    36961 aggagaatat acagcatttg atgtacgatt tactaatttt ctctttatcc cattagtaga
    37021 tatattcata tctattgcag gatttgtagt taatgtatat atcattgtct tcccccttgc
    37081 ttgtgctgtg tatgttttca ttataataaa aaagtctatg aaaatctttt cacaaataga
    37141 aaatcgattt tcatagactt ttcaatttaa ttttcagaat tattatatct gaccatataa
    37201 tctataatta tcctagatat aatatacaag ggcaatctgg aagatacttc atattcagaa
    37261 attgatatat gttcatgttt gtatcctaca atatataaat ccgaatactt tataagtttt
    37321 gcactttcac tacaacagca agttataacc ttagctccaa ctaaattggc attctgtgct
    37381 gattctacaa gctctattgt atttccattc aatgaaaata taattataag ttcgtctttt
    37441 tttattgatt tagattttat tttcattatg ttggggtcat ttataaaaac aacattaaaa
    37501 tcaagtaatt gcaatttaag actaaactct tttgctacat attcagtaag ccctcttgct
    37561 aaaatatata tcttactaga tttatttaca gtttttatta tattgattag tgtagttaaa
    37621 gatatttgtt ctataactct ctgtgcttct ataaaggatt tatttattat atcccccata
    37681 ttttgtatat catcattttc atcttttact gttaatctat acctaagttc attgaatcca
    37741 tttaaaccac attttctaat tgctcttgaa acggttgctg gagatgaata tgtttcatga
    37801 gcaatctcta ctatagataa ttctggtaat attgattcat tttcatttat aaatttaatt
    37861 atttcaagtt ccgtcttggt tagattttca gaattacttt tgtttagctc aattatcata
    37921 tgttatctcc ccttatataa gttttatttg tctaaattta tcgagtaata taatatatta
    37981 cctcttaact acttattttt atatacaatt aagatttagt caatgtctat atatgtaaat
    38041 ttataatata ataatttttt gcttaaagtt tattatgtta tcttatagca ttttaaaata
    38101 aaaaagaatt gtacctctac caaaatttgg atgaagtgca atagatattc cttatactga
    38161 tatgtttatg attaaaagta taaatataaa tttcgctatg aatcatataa ttaataaatc
    38221 attttattta caattataac cccccttata aaactatatt aataatatat acagtattta
    38281 taaggggggt tgtttatatt taaaaactat ttacttttct tttaattctt ctccattagt
    38341 tcttattaca ttttgatacc aataaaaact atctttttta taacgttttt tacttccatt
    38401 tccattgtta tctatatcta catatataaa tccatatctt ttttccattt cttgagatga
    38461 acaacttact atatctattg gtgaccacat acaataagct aatatttcgg ttccatcagc
    38521 tatagcttca cctatttgtt ctatatgttt tcctaaataa tctattctgt aactatcatg
    38581 aacttttcca tcttcaaatt tatcatgcat tccaaatcca ttttcagcta tcattattgg
    38641 cttttggtat ctatcataat attgagataa agtattgtat aatccttttg ggtcaattgc
    38701 ccacccccaa ggatttgctt ttaaatatgg attctttata ttgtcagttg gattcatggt
    38761 gtttttactt gctgatactg ttgtagaata atagtatgat aaagctagaa aatccatagt
    38821 attttctttt aagattttat catcaccttc ctccataact atatttatgt tattttcttt
    38881 gaaaaatctc ttaatataga ctggatactc tcccctaaaa cttacatcag tgaaaaaata
    38941 ttgtagccta tttcttttta aagcaaatac tacattttca gggtcacaat cttctggata
    39001 agatgtacaa tctgccatca tcatacctat catcatatct gaatttattt cttttccaag
    39061 cttcttagtc aaagctgtag ctatgaattg atgatgaata gcttgatatc tagtctcttc
    39121 tacattatct acttggtcta cacaaaatgc aactgaatta aatgattcat aatgtattaa
    39181 atttatttga tttataacta tccaatattt aactttattt ttatatctat ttagtattaa
    39241 ttctgcatat ctaacaaaga aatctaccac ttttctatta gaccatcctt tatattttaa
    39301 tactaaattc aaaggagtct catagtgaag tagtgttaca ataggttcca taccattatc
    39361 tattacttca tttataagat tatcataaaa ctttaatccc tcttcattag gctctaaatc
    39421 atctccattt ggaaatattc tagcccaatt tattgaaaat ctaaaacact ttaaccctag
    39481 ctctttcaaa agctttaaat cttctttgta tgtatgataa aagtctattc cactacgctt
    39541 aggataatat ccattattat ctttcatagc aaactttatt ctttctatag tcatatcaga
    39601 ctcataaggt ttagatatat ctctatcttt atcatacata tgtacatcag ctaaacacca
    39661 acctttgcca ccttcattcc atgcaccttc taattgatta gctgctacag ctcctcccca
    39721 taaaaaacct ttaggaaact tacccaattt aatcactcct tattttattt atttaaatca
    39781 tttactatta tttaccttat attctataga ctttactatt cctactaaag acatagttat
    39841 atttaactcg ctgaatatgg tcataagagt atcttgagca tgtgcaaata acagcgaatg
    39901 tgctcttttc tctccaccag cttccccttg tataatcttg gtttgagctc tatgtgcttc
    39961 tgtcataaac ccttttgctt tagctagttt taaatcagca tttctataat caaacttttc
    40021 caaatcatta agagcatgtt taatttctgt tctagcctca cctgcacgaa gtattatttg
    40081 cattgccacc tctactaatt cttcatctat tatatgttct tcaattgtca tatttataat
    40141 ttcctttcaa tttctaaatt taaacctctt ggctctctaa ttttatctct tctttctcat
    40201 aagctttaaa aaatggatac catatcataa atagcaatac tatatttatt gctaaaacaa
    40261 taagacctct aacatcttga tttaccgtaa aagtagatat accaaaagga acataccata
    40321 aattaaacac cttacttgga attgtcacaa atcccatact cattgataag taagtagtta
    40381 ttgatattac tactgttcct atccacatag gtaacattaa aaatgggttc catgccactg
    40441 gagctccaaa tattactggt tcatttatat tcattataga agggacaata catgctcttc
    40501 ctattgcttt tattcttcca actctggata aacacatcat tataactaat ggtaatgtag
    40561 cacccaaccc tcctaaagtt atccaacctg tttgaataac ttcgtaaaca tatatattag
    40621 ttgctactcc tcctgatgct actgctgctg cattagctgc aattgcacca agttgaattg
    40681 aataagttat aggagatagt acccacccac ttattcctaa agaatatata aatactacta
    40741 aaaagtttaa gagtacaaat ccaggtaatg tctgtgctat atctgatact ggtttaaata
    40801 ctctaactat tatttcaaac atgtcaaagt tgagtccata aattaatccc cagcctacaa
    40861 acattatcac tgttataggt aacatattat caaaccactt gcttacaaaa tccggaagtg
    40921 ctgaatcttc tccgaaaaat gaaaaagaac caaataagga cattattaat ccagagaata
    40981 atcctgcaac tagcgataca agcattcctt ctcctccaaa cctgttgaag ttaaatatag
    41041 ctccttcatt cgtaatctct ggacgaatca tcattaacat taatccagca cctgcaagac
    41101 cagcgactac actcatactt tcaaatttct tattcttcat tatttccata ggcaataaaa
    41161 atgctataaa taatcctaaa agtccaaatg tatattgatt tattggactt aaatcaggta
    41221 aaaagcttat gtaattcttc aagacagaaa ctatagttac taatgagcct acaaatacca
    41281 ttggcaaaac tttcattata gttgattgaa ctgattgtac ccatatattc ttagtcatat
    41341 tatttacaac aggagtaaat ttttcactta aaaaatctat aaatttattc ataaacactc
    41401 tccccataaa tattttattt gtttgatatt agttctagtg cttcttccaa agcctttgcc
    41461 ccatctagtc ttccatatgc agttttagat atacatgcta ctggaacgtt atattcatta
    41521 gccttttgtt gaatttcttc cttcatataa tctaaatgtg gtcctagtaa taaacaatct
    41581 atttctgata aatacttatc tatctctgac tcacttctcg cttttatatc tgcttctata
    41641 ccttttttct ttgcactctt tctcatacta gttgccataa aacctgtact tgctcctgaa
    41701 ccacacgcca aaagcactct tatatttttc ataatgatac ctccaatatt tttatccatc
    41761 aatttatttc ttaatgaact ttttatttta ttatgtttat attgtactta ccaaataagt
    41821 tcatttcaca taaatatatt tcctaataat aggaaatcag ttactcttaa ttaaacatat
    41881 taatatagta taatgtatct tataatacgt acattcaatt atataattat catatcatcc
    41941 agattttatt ttaaaactat gcgagggaat ctaatgaaaa catctaaaaa agaactattt
    42001 ttaaatttca taaaaacaca acatgattgg atagattcat ctacattggc taattatctt
    42061 aatgtctcaa cgcgttcaat tagaaaatat gtaaacgaaa ttaattccaa tggtgaattt
    42121 atattatcct ctaaaaaagg atataaagta aatctcaata ataattgtca aaccaaggtt
    42181 gatagttcag aaaatatatc acctgataac aggttaaatt taattttaaa agaacttata
    42241 gttaatagta atggaattaa tatatttgat ttaagtgaag acttgtttgt tagtccagct
    42301 actattgaag gagatatagt caaagctaat aaatttatag gaagttataa tttaaagata
    42361 aagcaatcga aatttttatt gaaactaatt ggaaatgagt ctgcaaagcg caaattaatg
    42421 agctctataa tatttaaaga aacgggaagt gattttttaa gcttatttga tgtacaaaaa
    42481 atatatcaag agtataattt gaccaaactg aaagaaaata taatatatat attaaaaaaa
    42541 tacaatttgt ttataaatga gtatgctata aataacatac ttcttcattt aatgataact
    42601 atagacagaa taaaaaagaa taactacata gaatctgtcg aagtagtaaa ctacatagat
    42661 aataataatg atataagcat ttcaagagat atatcaaatt ttttagagtc tgaatacaat
    42721 ataactttaa ctagtgcaga attatactac ttagtatttc agttaacaaa caaaaccaca
    42781 gtcttaaatt atcatgaaat ggatactaag tcattgccaa attatataga tgaacatttt
    42841 gttaaattaa ctaaaaaaat aattaaaaat gtatatgact tatactttat agatttatct
    42901 gatgatgaat ttgttgtaaa atttactctt catgtaaaaa atttaatcag tagggctaaa
    42961 aataatcaag ttttgagaaa tcaaatacct cagaagttaa aagattccta ccctctgata
    43021 tatgatatat ctgtttatat ttgtaatcag attcaaattt tagaaaacat agaaatagat
    43081 gaagatgaaa tatcgtatat atctcttcat gtaggttcat tttttgatag acaaaaatta
    43141 ctagaagata aaattctctg tgcattaatt actccaaatt actatgattt acaattcaaa
    43201 atagtaagag accttgagaa gagatttaat gaatccatag aaattattca gattttttca
    43261 gatactcata atcttgattt tgacaataag gtagacatgg ttattactac tttaccaatt
    43321 agcgatagat gccctatacc ttttgtttat gtaaatcctt acctaaatag aaaggattat
    43381 gataatattc aaagtaaatt tattcagatt aaagatagaa aaaatatact tactgtacaa
    43441 aatcatttag aaatgtactt tagtgaatct ttgtttatga aaaatatata tttagatagt
    43501 gctaaagatt atataagatt tatgggaaat attctgtacg aaaataaata tgtcaaaccc
    43561 aattatatag atgatgttct tataagagaa aaaatgtctt caactgcatt taataacaat
    43621 gtagcaattc ctcatagcat gaaaatggat gctttaaaaa ccggagtatg tttaattgtt
    43681 aacgataaac cagttaaatg gggtgaggaa aaagttcaaa ttatagctat gatacctata
    43741 aatgaaaaag aaaaagaaaa gttcaattac atatttgaaa gctttattga aatattatct
    43801 gagtggaata atgttaaaga acttactaag gcagataact atagtagttt tatgaataga
    43861 atagcttatc ttattcaaaa tatctaaatc aattaattta tttccaatat atgtttctta
    43921 agttattaac ataaaataga taagctgtca cactaaaaag tctaaattaa tatgtgacag
    43981 ctttttacct aatatacctt gttttaatac tacatatttt ttgactgttg tatactagtt
    44041 ataagattga atatttgtgt tgttgtagct gtcatattct cactagcagt tgctttatcc
    44101 attgcttctt ctattgtcat agctctattt actatagaaa aataagcatc tataccctct
    44161 tcattacatt taactgcatc ttctgttgtg gctccagcca aacctattac ttttacccca
    44221 tatttttttg ccagttttgc aactcctatt ggagccttgc ccatagcagt ttgattgtcc
    44281 aatctacctt caccagttat gacaaaatct gcatctttta actcttcctc taatttaatt
    44341 tcttctaata ttatttttat tccagattct aattttgaat ttaaaaatgc taaaaaagca
    44401 aaacctaatc ctcctgctgc tcctgctcct tctacattag ctacatcttt acttaagtaa
    44461 tttttaacta ctttggaaaa atttctaagc ccattatcta actcctcaat tatttctgat
    44521 gttgcacctt tttgagaccc gtatatgtat gcagctccat ttttcccaaa cagcggatta
    44581 tttacatcac aggctatttt aaacttacat tcatctaatt cctttaatct attttctatt
    44641 tttatatgtc ttatttcatt taaaactttt ccacctaatc ctactaactt gttattttca
    44701 tcataaaact caaaccctaa ggcttgaagc atacctatcc cagcatcatt agtagcactt
    44761 ccacctatcc caactataaa atttctatag cctttattta ttgcttcttt tattatttcc
    44821 ccaacaccat aagttgtagt gtataaagga tttctaagtt cagtcggaac caatgttagt
    44881 cctgatgctt gagctatttc tattattgct gtacttgtat tttttaataa tccatatgtt
    44941 gcatccactt ttttagctat tggtccagta acttctatag tatctttttt accattcatc
    45001 ccttgaacta aggcatctac tgtcccttca ccaccatcag caagtggcaa tatctttact
    45061 tgcgcatctt ctttaacctt caatatcccc ttttttattg catttcctgc ctctattgaa
    45121 gataagcttc ctttcaaaga atctatagaa attaatattt tcatatttat ctactccttt
    45181 ttattactcc ttattttaaa catataattt tataagctta atttatcttt tattttatac
    45241 tctacatctc tctttgtata caacttatac cattaatatt cttttatctt ataaaaaggg
    45301 agtgtctcaa aataaatttc ttagttcatg agacactctt tttgtataaa atcaaatata
    45361 tgtttattat ttcaacaatg ataaggagga ttatctctat tttaagataa ttccttttta
    45421 gcactattta accagtatag ttttaacttg gttcattaag cactctacat ttacagagtt
    45481 attatctatt gatatagatt ctaattcttt ttcatttagg ttaatccatt tagctgtttt
    45541 aatatttttg ttaaatacta tgttagctga ttttgtttct tctttagaat tgtaaaatct
    45601 aactactaat gcctcttgtt tttcagcctt ttttacaaca cttaacacaa ggtccttatt
    45661 agtttcctta agtaaactat atgaataatc tacttctatt tctgattgat tcaatttcat
    45721 agcattatat ggcattttat tataagtttg tatttttgtt aaatactctt tagccatatt
    45781 tgcaacattt gcttctagtg tagatttttc atgagtaact attgctaagt ctatttttat
    45841 atctcctatc atttgagaat ctggagtagg caactttatt cctgatggac gacctgggcg
    45901 tcttgccatt tcttctttac ctaggaatcc tacacttcta aataatgtta tagctattgt
    45961 gtcaaatttt tcccctacta tttcaaactc tcttgtactg tttgtaagaa cagctactcc
    46021 atgctctttg tcagacaatc caacgaatgt tagcattgga tatatagaat ctggtctttc
    46081 actccattct tcctgttgcc aaacttccat agcatcatca tacacacttc tctttatatg
    46141 tccaaactga ttatcagaca cagaaaactt tgaagctata tttgttggta tataagttct
    46201 aaccctacaa tctcttgctt ggttatttat gttgaatact acatctatta tagctttatc
    46261 atttggaaca tttataacta tatcaaagtc aataaatgaa tctgctctat tattttttct
    46321 agcttgtata tcttttggta cttttaatct ataatttatc tttatctcat tattaaatct
    46381 atttttcttt atagtggaat ttgctactat attatcgtta aatattaatt tctcatttgg
    46441 aagtggtgaa aaatcatact catctccttc atctcctcca ttttccatca ataatacatt
    46501 ttcaaaagtt ttatttaatt ttttatcaaa tatctttaat gtcccgtttt cattaacttg
    46561 aatagtataa aagtcattat ctaccatgtc acatacttca taagcttttt ctaacatatg
    46621 ttcaacttct tttatcatat acgccttata ccccattgca ggtattttat ctttaaagtt
    46681 tattgtatat accataaatg aatcatagtt tccataatga acaatttgac ggtctattaa
    46741 tcctgcatct attacttctt tatgtaccac ttcaaaatcc aacttattat tatctttatc
    46801 aaatatttca aatgctttca ttttagttat aacttgagcg cttactattt cattcctatc
    46861 gtaaggcatt aagttaaatg ctactaactt atctaaagcc atttcacaac tcattgcatc
    46921 aactattctt cttttgtaaa atgttattaa ttggtctact ttttcctcag ctaagaaaaa
    46981 tctattcatt atttcatggt gaactttatc tgaacaacaa catccaatgc tgtcatgagc
    47041 atgatttttc attatttctt tccaaattaa ctctattaat ccatgatgat attcaaatcc
    47101 tagagaatat gcaatagaag ctagtggctc caatatgttg gttatctttg attctattct
    47161 agcatttgct gattttatat ccattcttgt tgagaatata cttctatgaa ccctcatata
    47221 tttcccatct aaaaattccc cttttattgt atctatatcc tcattttttt ctagctcttt
    47281 aaatatgttt tcatatctac ttaagaaaaa ttttctctct ggataacatt cttttaattt
    47341 atatataact tcaaatatat tcttttgaat tggcatttgg tcatgtccat ttggaagtat
    47401 tatatggtca gtagttgcac ccttatcaag aaccggtaaa tatttatcca ttcttgttct
    47461 tagctcatct tcattcattg gtagatattt tcctattgca taacctaatg gaagtatttg
    47521 aaccaaaaca gaagacccat catcactctt ccaattaaac tctgttttat tagttccttt
    47581 tctttcagaa catcctctcc aaaacattga gtattttata tcaaacccat ttagtatttg
    47641 tggcatttga gcactttgac caaatgaatc tggtaaatat cctattttca tatattctcc
    47701 aaattcatca caatctttta ttccatatag taaatttcta agtatggatt ctccaccaac
    47761 taccatttca tcagtttgag tataccaagg acctattatt agccttcctt cttgaactaa
    47821 tttttttatt ctctccttac attcaggctt aactgctaaa taatcttcta atatagcagt
    47881 ttgaccatcc atcacataat atggatagtt atgattattt tctaacatat ccattatttc
    47941 ttccatatta tttacaagta atattctaga ttcctcagtt gagaagtacc attctctatc
    48001 ccaatgcata tgaggaacta tgtgtacatt ttttttcatt gcttatcctc caaaacttat
    48061 ctgataatat tttctgtcta attaaaaatc caataaaaat actactgagt ttacaaaatt
    48121 ttttaatatt tattttatca ctataattaa acctttgatt taaatatcta ataacgtatc
    48181 ttttaatgta tctttctttg atttttttgt ttcctagtta ttattaataa tactgctgat
    48241 attatagctc caagtactgc cgcaaagaac catattgatg ctataataaa tcctgacata
    48301 cttccctcta aaagcgccaa tgagaatatc cccgctccag gcacttgaag acctatattg
    48361 aagaatgcta ctatagaccc agttatggct gaaccagcta ccaatgatgg tataacacgg
    48421 attgggtcct ctatcataaa tggtattgca ccttcagtta ttccagccaa tcctaatatc
    48481 caagtttgct ttcctatttc cttttctgat tctgaaaata aatctctgcc taaaacagta
    48541 gctattgtaa ctccaaaagc tgaaaccatc ttaactgatg caaatattgc ataaggtata
    48601 aaatttccac ttgctattgc tcctattgca aatgtatagg ctgctttatt tattggtcca
    48661 cctaagtcaa atgaaaccat agctcctatt attgcaccta gtagtattgc atttactcca
    48721 gtcatcccat ttaaccaatt tataagacca ttgttaagtg ctgctacagg ctttcctact
    48781 acaaataaca tcaataaacc tactaccaaa gaaccaacta ctggatatac ccaaaaactt
    48841 acgaatccag ctaatgtgcc tttggctggt atattcttct ttaaaaattt tactatataa
    48901 ccagcaagta aacctcctaa catacctcca agaaatccac tacctattaa atcagctgct
    48961 agacctgctg caaatcctgg tccaagacct ggtttttcag ctagtgagta agacatatat
    49021 gctgataata ctggaaccat caatgtacct aacatacctc cagaaagctt tcttaattgc
    49081 cataaccaag aaccttcttg attgtatagt tcttgtagac caaatgcttg agatgctaat
    49141 actgcaaatg caagtatcat acctcctgcc acaataagtg gtattatatg agatactcct
    49201 gtaagtattg ctgattttac atcttttttc catgatgatt tttcacaata tgatatatca
    49261 gatgattttt ctatataatc acctttacct tgtttttgtg ctacttctaa agcttcgtat
    49321 attatttctt ttgctctttt tagtggagct gctactgacg tttttatgtt tggcaaatgt
    49381 tcaaatcttt ctgtattttt aggtgctaca tctgttgcat atattattgc atctgccctt
    49441 tttattatat ctggtgtatg agggtccacc ataccattag ctccttgctt ttctacatac
    49501 acatccacac cgatttcttt accagcttta attaatgctt ctgctgacat atatgtatgt
    49561 gctatacctg cagggcatgc tgttattgct aatataattt tttttgattc attttgaatc
    49621 ctaatctctt cttttatttg ttctttcttt tcttttatat ctaagttatt aaataactct
    49681 ttcttagttt ttgaatttaa caacatattc atataatctt catttgacag tctagtaact
    49741 aactcagaaa gtatttgtat gtgtgtattt ccaccttcat tttctggtac tgctattaaa
    49801 aatattatct ctactttatt atttgggtct acgctttccc aattttctat tggtgtctct
    49861 aaggtcatag ctgcaaatgc agcttcttta actgacttag attttccatg aggaattgca
    49921 agccctcttt caaaacctgt tggagataaa gtttctctgt acattacagc ttctaaatat
    49981 tcctcataag atgttaactt gccttcatca tatagctttc ttgctaagaa ctttattgcg
    50041 tctttctttg tttttatttt ttgttttaaa aaaacaaggt tttcatttgt gattttctta
    50101 aattccaaaa ttacgccctc cttaaatcat ttaatgctat gaatatagca taaaataaat
    50161 tatgaaaatg tttttttgaa attatacaca caatttcttc ttaaaaaata aagatttgtg
    50221 tatttataca caaatctcta tcttaaccag tattgctcta ttattgctct cataatcatc
    50281 ataattggaa ccttatctga tatattgtac ccatttactt gtttaattgt tgaatagcaa
    50341 tataaactta tatctgcaat ttgttgcaat tgattttcac atatatgtgt tagagctact
    50401 accttacaac ccttattaac tgctgtcttt gctacttcta taacttcttt cgtttctcca
    50461 cttaaactaa gtgcaaaaaa catatcttgt tcacctaatt ttcctatatc atgaagtact
    50521 tcatgtcttt gactatagta agttatatta tcatgcccac ctctaatttc ttttactaat
    50581 atctcacata aaggaatgtt ttgacctatt ccataaacta taatactcct agatttttga
    50641 attatatctg ctactttatt tattttttca taatctatta gctctaatgt cttttttata
    50701 tctgattcaa aaccattttt tggctcttta tcctcatttt ttaactcttc ttttaaatta
    50761 ttttttagtt gagagaaacc atcatactcc atttttttac atagcctaac tatggtattt
    50821 gggacagtaa aaaatttatc tgctaaagat tgtattgata attttactat ttcttctttg
    50881 ttttttctca tatattcaat tatcatgtct tctgtatctg taaatttata ctcatatttt
    50941 tttgctcttt cataaaattc catagtttct cctccatcta ttttattatt cataagtaaa
    51001 attcaaaaat attagctaaa attttcgtta atataattat aatttaatat ttttaattta
    51061 ttaatgcata ctactctttt tattatacat aaaaaatgtg tacttcctat attgatagag
    51121 atgattcaag tttttatttt gatacatctc tatcaatatt tttttagaaa atacacattt
    51181 ttttcaaata cttattttta attaaaactt taggtaatct attctttata tataattatt
    51241 agacttgaaa atatctttaa aatcaaccaa ttcaatatta tttcttttta accacatttt
    51301 aacttcctta tcacatagca ttgcatgttc atatactctc tcttgaataa gagaagatga
    51361 ttttataaga ctataatcta taaatcctgg atgagtaacc atcaatgtta tatctttatc
    51421 taaatttata tatttttctt caaataattt aacaaaagat ttatttaact tgtaaaaatc
    51481 attctgagga tatttacttg gcatgctaat acatgaatcc aaataatagg gacaacttgg
    51541 tataccgtat tcttttgcag catcacacat tgctgatatt gatacatctt cttctatacc
    51601 atgtatatca atatactcag gcaatttact catatacaac ttgtaagttt caatttgagc
    51661 acaaacttct tcatatactt cttcataatt aaaaacctct ttacctgtca gtagttgcgt
    51721 tcttcttatt ttgctagaaa caaattttcc accttcatct aacaaattct ttatcttaat
    51781 atgttctttg caacaaggtt ctcctattac taaattaaca tgtaaaccaa aacatacatc
    51841 atcatttttt accatttcta tagcttgtct tgaaaaaggc atattcacca tgattccagc
    51901 acttgtaaca ataccgttct tataagaaga tattatccct agtgtaacac cttctgaata
    51961 tcctatatca tctgccctaa ctactagttt cttcatttta tgcgtctgcc tgctttctca
    52021 actcatcaag ttgcatttcc atctcatcct ttactttaac aacatccgtt cctatgatga
    52081 tttgaactga tgtattactc aatttaatta cccctttggc tcctgttttt ttaatattat
    52141 catcattcaa aatagtagca tccttcactt ctaaacgtaa tctcgtcata caattattaa
    52201 gagtcactat attttcataa ccaccacaat ttttaattat ctgtttagcc atatatgaaa
    52261 aattaccatg agaaagtttt aaattctttt ctttttcact aactttatca cctatctcta
    52321 tctcaacttc acgtcctggt gtttgcacat tatcttttat tattatatat ttaaatgtta
    52381 cataatataa tacaaagaat acaactccta caggtataac taaccacgct ttatctccat
    52441 aactaaaact taagacatag tcaattatac ttgctccatt aaatgaaccc aagcgaacac
    52501 ccagtaaata tagtacaact ccacctagac cagaataaaa agaatgaatt atatataact
    52561 ttggagatgc aaataaaaac gaaaattcta ttggttcagt tactgttgcc ataaaagaag
    52621 tcaatgctcc agcggtcatt gcacctttta cttcttgctt ctttttatct tgtgctgttt
    52681 tatatatagc ataagaaata gcaggtattg aaaacatcat aattatgtaa aatcctgtcc
    52741 agaagaatcc tgcatttggg tctcctgcca taaatctagg aatttctcct cttataactt
    52801 ctcctgttga tgttgtatat tcacccattt caaataatat gtaagcattt aaaacttgat
    52861 gtaacccagt tggtattagt gcacggttac agaataaaaa caagaatatt cctattgcac
    52921 cagctcctcc caaccaaaaa ccaaattgat tcattgcagc ttgtggatat atccataata
    52981 aactaaatat atatgataaa attaaagcaa ccactggtga tagtgtaatt acacattttt
    53041 ctcctgcaaa gaagcttaaa aatgctggaa ctttaacttt atatgtttta ttgtaagtcc
    53101 atgctataca agctccaaca attatcccac caaatacacc tgcatttaat gtatcattca
    53161 ataactctaa atttgactta aatatcatat atgcaactac ggctgcaacc attacacttg
    53221 tcttgtcttt agactttgag taggcactag ctgctcctaa taaaaataag tagtccacat
    53281 ttgaaaatac tatattagat atattagata tagctggcac attgagcata tttggaccaa
    53341 ataataatcc taatatacct gctgctggca ttacagctat tggcactagc atagtctttc
    53401 ctaaaagtga cagttgttgc atcattttgt ttaagaacct cttcatatcc aatccccttt
    53461 ctatttcggt ttaatttttt gtgaaaacga tttattcaca ttttatattt taaatattat
    53521 atttgtttaa acaaattgtc aatatttttt attcatttta tttttatcat ttagtttaac
    53581 atataaatcc ttttaagtgt attgctatat ctagtctatt tttttaattc atttttttta
    53641 ttttaaaaat atgtttaacc atgtatttga aacaacccac aaaataattc tattttgtcc
    53701 tttttatggt tgatatgatt ctattttcac tatacatgtc taaacacatt tgatataatt
    53761 tgttcatatc agttcaataa tattactaaa gacctttaag gagggatagg ggtatatggc
    53821 taaaaataaa ttttcagaga tatatgaagt attgaaagag gaaatacttg atggtaaata
    53881 tacaagtaat atgatgctgc ctactgaact tcaacttatt gaaaggtttt catgtagcag
    53941 aaacactgta cgtagagcaa tttctcaatt aaacacagaa ggatatgttc aaagtataaa
    54001 aggcaagggt gttgtagtgt tagaaaatag ttgttctaat gatttttttc taaatatgca
    54061 caatttcaaa ggtgtagaat cgattgtaga agataaaaag gttaatactg ctactagtgt
    54121 tctccacttc agcaaaatct taattgataa taagttatct aaaaagacag gttttaaagt
    54181 tggtagtgaa gtatattacc ttcacagact aagatatatt gataacatac ctaaaattct
    54241 cgacattaac tattttctat gtagtatagt taaagacctt gatgtttcta tagcacaagg
    54301 ctctatttat aaatatatag aagagtgtct aggtacaaaa attgtttcat ccagaaagat
    54361 atttaaaatt gaaaaagcta cagagcttga gttaaaaacg ctacctctaa atgattataa
    54421 ttgtgttggt gttattaaaa atagtgtata tactgatgat ggaaagcttt ttgaatatac
    54481 tgaatcaaag catacacctg aaacatttgt attcatggat gttactcaaa gatattaaaa
    54541 aaattatgtt taattaaaca taattttttt acgtttagac actttatccc atactaacct
    54601 cactattcta tatacaatta tgtgtttata caaataatta tttattgtat attttagctt
    54661 taatattagt ttaatgcttg acaatttgtt taaacatatt ataattaact tataaattac
    54721 aggaggtatc aattatgaaa aattggtgga aaaaagcaac tgtctatcaa atatatccaa
    54781 aaagtttcaa agatagcaac aatgatggta taggtgatat aaatggtatt atagaaaaat
    54841 tagattatct gtattattta ggtgttgatt tattatggtt gactcctatg tacgtttctc
    54901 ctcaaagaga taatggatat gatatagaag attattataa tatagaccca aaatatggga
    54961 caatgagtga ctttgaaaaa ctcttaaaag aagctcataa aagagacata aaaataatga
    55021 tggatatggt cctaaaccat acatctactg aacataaatg gtttaaagaa tctaaaaaaa
    55081 gcaaagacaa tccatatcgt gattattatt tctggaaaga tgctaaacct gatggttctg
    55141 ttcctaataa ctggatatcc agatttagtg gaactgcttg gaaatatgat gaaaccacta
    55201 accaatatta tttacattta tttgaagaaa ctcaagctga tttaaattgg gaaaatgaaa
    55261 aggttcgtga agaatgttac aaaatactag aattttgggc tgataaaggt attgatggtt
    55321 ttcgtttaga tgtagtcaac ctactctcca aaacacctgg attacctgat gaccctataa
    55381 caggaccaaa aggagatgga agaactcact acgctgatgg tcctcgtata cacgaatatt
    55441 tacataatat gaatcaaaaa gttttcaaac ctaaaaatat agttactgta ggtgaaatgt
    55501 catctactac acctgaagag tgtatcaact atactagaga aaatcgagaa gaactatcta
    55561 tggtttttag cttccatcat atgaaaacag attataaagg aggcgcaaaa tggactaatg
    55621 aaatgttctc tctggataaa ttaaaaaaag cacaatctga ctttcaatat aaaatgtatg
    55681 aaggaaaagg atggaatgct ctattttact ctaatcacga ccaacctaga gctttatcta
    55741 gatttggaga tgatagattt tttcgtcaag aaagtgctaa gatgttagcc ataacactat
    55801 ttggtctaca aggtactaca tacatatatc aaggtgaaga aataggtatg acaaatgcct
    55861 actttactaa aatagatgaa tatgatgatg ctgaaagtaa gaatgcatac tatcatatga
    55921 ttaaatctgg tattgatgaa aaggaggcat tgcttatact tcaacaaaaa tccagagata
    55981 atgcccgtac tcctatgcaa tgggataata ctatttataa ggggttttct aatcataaac
    56041 cgtggttaaa agttaataat gacaatatta gtgtagaaaa tgagcttaat gattctcgtt
    56101 cagtatttta tacttatcaa agattaatca aatatcgtaa acaatatgat atttttacag
    56161 aaggtactta tagattgtta gatgaaaatc ataaaaatct atttgtttat gaaagagagt
    56221 ataaaaacca aaatcttctt gttataagta atttcactca tgataatgta gtatataaat
    56281 tacctgaaac ttatttgaat aaattaaact acacaatact tgaaaccaat tacactagag
    56341 atataattga aaataaaatg gaaataaaac cttacgaaag tataatgata tattataaat
    56401 aaagttattt atgtaaaaat aggtataaca ccctggtgtt ataccaattt tactaaattc
    56461 aaagtaatgt ttatagaata tctatagatt acaataatta tttgacttct acatcattat
    56521 atttctttga atatacttta gtcatataga aatatatagg aactacaatt atcatcccac
    56581 ctaatcccca aactatcttc ataggttcat caatacctgc ttttatcagt aataatatac
    56641 ttataataac tgcaaatatt ggtataattg ctcctccagg tattctaaaa tttacatctt
    56701 taccagcata cttctttctc aatattagta cagctaaaca tgtaggaata tattgaataa
    56761 atctagatac aacacttatt tgagataatg ttgtgaatga acccgaccat gctattagta
    56821 aagttcctat gacagatact ataattgcta tataaggagc tccattttta ttagtttgtc
    56881 ttatagactt aggcattaat ccttgttcaa ccaaagcagc agcagacctt ggtgtaaata
    56941 tggatgatgc aatatttata cctccaacag atactatagt tcctattgta attatatacc
    57001 cacctatagg acctaacatc ttaatggcag catctgcaac aggagtagct gaccctgcta
    57061 agccattacc caacacccct atacttattc ccagtataag catatatatt actgatacta
    57121 caaacattac cattaccaat gctttaggta catttttctt agggttctcc atatcttttg
    57181 ctgcaacagc taaagattca aatcctgtaa aagcataaaa taaagttata gatactgcta
    57241 caaaagctga tgacgcactt tgaccttttt gtattattac aaatggggta aaattagaac
    57301 cctttataaa gaataatcct acagctataa ataaaactat tggtaataat tttcctatag
    57361 tcatgatatt atttaagtgc ttagattgtt tcattcccat aaaatttata atcccaagcc
    57421 ctacaactaa tataccaatt attatatttt taataagtgg ttctgctgct tgtggccata
    57481 cagatgatag tgttacagca aagaaatttg ccatagtagc ccaagcaatc atactaacta
    57541 tccacttcat aaatccaact tcaaatccaa caaaatctcc aaatgcctct tttgcataaa
    57601 gatagggccc tccatcagta tcaaaccaac tacctgcttc agcaaaacat aaagcaatag
    57661 aaacagctaa aagcatacaa aaaactaata ctaatatact tgctggacct atatctgtat
    57721 aagcatcacc aggtaacccg aatatacctg accctattat tgcattgatt cctaatagta
    57781 caatactcca aaaacctaac ttaatgcctt tttccattgt tattcccccc aaagccaact
    57841 taatatattt gtatttttta tttagatgct tctttaacta ttgccataaa tatttttttc
    57901 atattatcat aatctctagt catcatttct ggatgccatt gtatccctat tacaaactca
    57961 cttccttcct tttctatagc ttcaattaaa ccgtctttag atgtagctga aacaatatat
    58021 cccggtgcta ctttgttgac aactaagtga tgaaaactat taactaaaac ttctttttct
    58081 cctagtattt catataattt tgtcccctcc tttatctgta ctgtatgtgt aggtacatta
    58141 gatagatgtt gctgattgtg tttaatataa gcaccttcaa ttaaagataa atcttgatac
    58201 aaactacctc cttcagttac atttataatt tgttctcctc tacatatacc taaaactggt
    58261 tttttcatat ctaaagcatg ttttaataat ttcatatcaa aactatctct ctttggtgat
    58321 attgccccca acttattatg aggttcttct ttccatatga gtggatttac atcttgtccc
    58381 ccagatagta taagcgcatc tatattagat acctgctcct ttataatctc atcatcatat
    58441 acaatcggta caatatatgg tatagcttta cacataacta ctgattgtat ataatcatta
    58501 tttacataag ccctttcata gccaggaaac attcctcctt catcaattag taaattacct
    58561 gatattccta ttattggata tttcttttta gccaatttct tcacccctta acttatttgt
    58621 gaacaataaa aaatgctact tctcttaaag agcaagtagc ataccaagat tttatttatt
    58681 tgaacattat aatttatttt agaaatactt tttttgaata cgagtaatat aaataacttt
    58741 atttacttat attttatttt aatcctgttt agtttgactt ttttaaattg ttttaagtac
    58801 aactaatagg taaaataggc atttagattt tcctttttat caacctatta aatactgttt
    58861 aatatatttt ttccccttct atttaattca cttccctttc taccagattc acatattatc
    58921 aaatccagct cttttaacat attaagtatt cctcttacct caccttctgt tatattgtag
    58981 cctaattcat ttattgaatt aacaatacca tttctaccta tatttttatt aagattattg
    59041 gcatttttta aacactccat tactaacttt actttctcta tagatgttct atcctttaat
    59101 atgcttattt ctttactaaa atcttcagtt atatttaata agttatgagg taaatcattt
    59161 attaaaataa catcctcaga actcataatg ttaatataca tagcagtatt ttttaattct
    59221 cttatattac ctggccattc atatgcatta ataatgtctt gaacatcttg actaattgct
    59281 ctcttccttt tcataaaatg ctttaacata acttctatat cttcttttcg ctctcttaaa
    59341 ggtggtatat ttatgggcaa tacatttatc ctatagtata aatctgctct aaatctatct
    59401 ccttttatca tttctaataa atttttattt gtagccgcaa ttactctaac atctatatct
    59461 attacatttt ctcctccaac cctcattact tgattctctt gtaatactct aagtaatttg
    59521 ctttgtaagt atataggcat gtctcctatt tcatccaaaa atatagttcc attgttagcc
    59581 tgttcaaata atcctttttt acctcctttt aaagctcctg taaaagcacc ttcttcatat
    59641 ccaaaaagtt cactttcaag taaattttct ggcatagctg cacagtttat tgctataaat
    59701 ggctgactat gtcttggtga tgcattatga atagactgag ccatgagttc ttttcctgtt
    59761 ccactttctc ccgttataag aattgtataa tcagattcag ctattttcat acctaatttc
    59821 ttagttcttt ccatcttcgt tgaaactgtt tttatatcat caaaagtata tctagcaact
    59881 tgtccctttt ctctaagctt ctttgatagg ttttgctcca attttcttat gtaagttatt
    59941 tcttgaatac aaaaataata tccagtaacc cttccaaaac tctctatatt atacttattt
    60001 acgtttaagt acttattatt aaattttata acttcatcat gtgccttact caaactaaaa
    60061 atctttttaa tattctcatt ctctaattca tctatatttt tccctataat attactttga
    60121 atatctagta tatcccttaa actattatta caaataatta tgtttccctt atctgatacc
    60181 attgccatac ctaagtttga taaatttatt atggaattta gttcatctgc ttttatagta
    60241 agttctttgt atttagtatt tattcctgaa tataaactaa ttatttcatc tgtatactta
    60301 attagcctct cagtaatttc tttatcatcc agtttgagtt ttgctattat ttgaataaac
    60361 gttgaactat caatatacct atggcctaaa tcaataactt cttctatata ttttggaact
    60421 aaacttgact ctcctggcgt tatagctatc ttaatatttc tatattcttc attgggattg
    60481 tatggtataa aattcaaatg atttattcct atattgtata aattagatat tgtttccaat
    60541 acagtatgct tattatcgtt tactactaat acatctattc cttctggcaa tgaaaataac
    60601 ttatatatat ccttttgctt tatgctccta tttatagtta ttatctttga gacatcttcc
    60661 acatattttt ttatttttaa tactctatca tctatcatta ccaatattat gtcatctttt
    60721 attaattcct tctctgttat tttatttaaa taataatttt ttatcattac tttatcttta
    60781 aagatcattt ctagattttt atttaagaaa cattctaatt tagacaaccc atcagttact
    60841 actgctattg ttttcatttg attactcctt ttaaaatata ctctactctt ttataatata
    60901 catttgattt tcactttaat aacacaaaaa attgtacatt tagtatacta acatattcct
    60961 ttattagttt tatgtccata aatatcatag ttgcaatatc tatcatattt cttaaacttc
    61021 tataaagcaa aaattgtgcc aaattaataa ttttcttaat tttattctaa agttcctcaa
    61081 tagtatttac agacttagca cttatagctt ataagtcttt atattattaa tctattttta
    61141 taggttaatt gtacaaatca tatatcactt ttacctatta aatgcattat aaaaaattgt
    61201 atttataaac actatttact actcttctaa gtacaaacag tatctacata ctataacctt
    61261 tcaccattag attctattac ttgtttatac cagtcaaaac tctttttctt aatcctagac
    61321 aagtctccac ttccatcatc atacttatcc acgtatataa acccatatct cttggccatc
    61381 tcaccagtag atgcacttac taaatctata catccccatg gagtatatcc aaataaatca
    61441 acgccatctt ctacagcttc cttcatttgc tctatatgtg cttttaaata ctctatacga
    61501 tagtcatcat ttatcactcc attagattct tttttatcat aagctcctaa accattttca
    61561 actaccatca taggtattct atatctatca taaatttcat ttagtgcata tctaagccct
    61621 tcagggtcta tttgccatcc ccattcacta gacttaagat atggattttt cactccacct
    61681 aatatgttgc cacttgtatt atcttcttta gaatcactac ttacacatgt agacatataa
    61741 taactaaatg tataaaaatc tacacaacct tcttttaaaa tttctaaatc tcctttttcc
    61801 atttttattt caatattatt ttctccaaaa tatctattca tataagttgg atattcgcca
    61861 cgcacttgta catctccaca gaaataattc attactctac tatctctttg agttttaact
    61921 gcatcatctg gattacaagt ataaggatac attgttgcaa atatttgcat acatccaact
    61981 tgataatttg aatctatttc atgagctaac ttaacagcct tagcacttgc tacaaattga
    62041 tgatgtaacc cttgaaaacg aagttgaggt atatctactt ggtttataaa atctgtagtc
    62101 ccttcattta atattccttg tgataagtag cctcccatag gcattgtagc tgaatttatt
    62161 tcattaaatg tcagccaata ttttactttt cctttatatc ttttaaatat tgtttcacag
    62221 taattcatat acaagtctat tacatctctt gaaacccatc cattgtattt ctctgttatt
    62281 gcaaatggta tttcatagtg agatattgtt attaatggtt ctatattata ttttaaacat
    62341 tcatctatca cattttcata aaatttaagt cctgcttcat ttggttcttt ctccattcca
    62401 gttggaaata ttctagtcca tgctattgaa aatctaaata ccttaaatcc catttctgca
    62461 aataaagcta tatcttcttt gtatctgtga taaaaatcta ttgcttcatg acttggataa
    62521 aatgttcctt tttctataac tctcgttatt cttttactta ctttttgaga acctccagta
    62581 cacatatctg aacaagaagg acctttccca tctaaattcc atgctccttc acattgattt
    62641 gctgctgttg ctccacccca taaaaaatct tttctaagtg ccataattct acctccaata
    62701 atttatttat agtaacttta ttttaaaata aaaatacata tatataattc ttaattaata
    62761 gaactctctt ttacgctaaa acagttatca atttctgact attatttaca tgcttttgtg
    62821 tattttctac aatatccaca taatcagaat aattagtaac aactactggt gtttctaagc
    62881 aatatccttc ttttgaaata ctttctatgt caaaagttat taatgtctga ccttttttaa
    62941 tcttatcccc ttgattaaca aaagatttga aatatttacc ttccaactga atagtattta
    63001 accctatatg tatcaatagt tcacaaccat tatcagatat aattcctatt gcatgttttg
    63061 ttggaaataa agtcattaca gtcccatcaa atggtgccac tacttttcct tctttaggtg
    63121 ttattgctac accttttccc aatatacctg ttgaaaacgc tgcatcttgt atctgctcca
    63181 aagcctttac ttcacctttt attgggctat atactacttc tcttttgttt ttttctataa
    63241 cgccttgccc attatcatct ttagaaattt ctactatagc atcatctttc caaaaaaata
    63301 atgttaaaac aaatcctact acaatagcta ctattattgc tattatagtt gcataaacac
    63361 ctttcatatc tccagttgtt ggatttatat aattaggtat accaaatatt ccaagtccac
    63421 ccattacata actttttaca ttcattatcc ccattacagc tcctgccaca gcaccaccta
    63481 cacaactata tatgaatggt gtttttcttg gtaaagttat tccatatatt gcaggctctg
    63541 ttacaccaca tatccctgaa ataactgcag gtatacacag ctcttttatc ttcttatctt
    63601 ttaatttaaa gtacattgct gctactactg ctgtttgtgc aaaagatggt acaaatactc
    63661 ccactaatat tgtgtcaaac cccatagtca taaggtttga catagctatt ggtactaatc
    63721 cccaatgtag accaaatatt acaagcactt gccacaataa accagttaat gcaccacata
    63781 aaattggtga aaagttaaat aaagctaaaa atccatttcc aactatatta gaagcatagg
    63841 tagctatagg ccctataacc ataaatccca atggtaaaga aactaataaa actgccattg
    63901 gtacaaagaa attctttact acatctggta ttattttagc aaacactttc tccaacttag
    63961 ctgctaataa tactattaaa attactggta ctactgttga agtgtagttc attgctatta
    64021 ctggtaatcc taagaaagtt atatatactg gtgattcaaa tattgttcca gtaaatagat
    64081 tgtataaagg ttgacctgta gatgctagag tatctaattg aatacctgaa tagcataaaa
    64141 ctgcacctat aagcattcca ataaatggtt ttaatttgaa tttcttagca gctgtatatc
    64201 caagaattat tggcatatac tggaacattg catctccaat tgcatttaac ataatatatg
    64261 caccatcagt ttctccatac aaacccaatg ctgtaaatac tgcattaaat ccttttatca
    64321 taccagcagc acacagaact cctaatattg gttgaaaaac tcctgatatt atgtctataa
    64381 acttattaag taatcctttt ggcgcatctg agtctgaatt atctattgta tctcctccta
    64441 atccagccaa atcgcacaca acttcataaa catctggaac atgattacca ataactactt
    64501 ggtattgacc tgcacttttc attacagtaa ctataccatc catttttttt agcacatcat
    64561 catttgctat tgactcatct tttaatttaa aacgtagcct cgttatacag tgagataaac
    64621 tgctaatatt atcctttcct cctacattcg caatgatttc ttttgatagt tgttcatact
    64681 ttctcatttt ttctcctcca ttttcttttt gaaatatgaa ggtttggcca acttaatgtc
    64741 accacccaat atatataaac ctaattatta ggatttatat ccgacttaat acttttactt
    64801 accactcttt caacgtgaat agttaagtat aatttttcct catctgttaa atcgtagttg
    64861 tattttttat gtataaattc atccaacttt tctatgaatt tataagcatt tttatattta
    64921 acttttatta catttaataa atcatcttca ttattttcat gtaaattatt actaacaatt
    64981 cgttctgcaa aatactttaa gtgagtaata aatctatgat aataaactga atcttcatca
    65041 aatactattc tgtaatggta tttaaccata tttgaaattt cttgtattat ttgtgtaatc
    65101 tcatatatag tttttatact ttgatccatt tgtgcattaa ctatatgcag tgctataaat
    65161 ccagcttcat cttctgatag tctaatttca aatttttttt ctatataatc caatgcctct
    65221 aaccctattt taaattcact cttataaaat ctttttatat cccaaagtaa tacatttttt
    65281 acacttactc cttcacgagc tctttctatt gcagtataca tatggtctgt taaagaaata
    65341 taaatactat catttaatct tttccctagt ttttctttag catataaaat tatttcatct
    65401 gataattcca tatatcttat aggaatatct gctattagtt ctttaaattt atcagatata
    65461 tttgggtctg aaagtttgaa tatcttatca attttatcct ttgatatatg ctctcctgta
    65521 cgttttttat aagctaaacc tctccccata actactattt ccttatgatt ttcatcagtt
    65581 gtaataatta cattattatt gagtattttt tcaataatca ttgtgatgcc ccctttatta
    65641 gcattaagtt aaaataaatc ttttttaatt atttagaata tttaaaatgt aatttattac
    65701 ataaaaaaaa acctatttat tacacattaa atagactccc atgagtcctg agtaataaat
    65761 aggtttagct tgtatttaaa caatcactat ccgtttgtat tttattttaa ttattattta
    65821 ttatatgtta gcaaatttat tttttagtgt caactttttc tcaatatttt aattttaaat
    65881 aaattatata aattgaatct tcaaagtact atttttatca ataaacaaaa taaaaaaggt
    65941 attactttct ttaagtaata cccatttatt tctaatttaa gtggtttttc ttaatctcat
    66001 caagtagtcc atcttctaaa taaatttcta atgcacatcc taccattgcc tttactgctt
    66061 tgtgaagttt atcataagct tcctcagaat ttgcataatt tagaaattct ttttcatgaa
    66121 catataccat tggacttata tctaaggcta tttctgtata catagtaggg caaacttgac
    66181 ttacattacc tatatctgta gaacctactg gaccatcttt accttctaat atatctgtta
    66241 tacctgcctc aatcaaatta ttctttgtta tcttttgaag tactttgtta tttattatgt
    66301 tgtcaaaaga attttcaaag tatctataag aaagcttagc tcctgtcatt aactctgcac
    66361 cttttgcaca gtttataact tttttagtta cttcatctaa ataacttctt tcagcagctc
    66421 ttacgaaaaa cttacaagct gctttttctg gaactatatt tggagcctct cctccatttg
    66481 aaactatacc atgtattcta acatctgatt ttacatgttg tcttagtgca tttatcccag
    66541 caaaagtaag ttgtactgca tctagtgcat tgactcccat atgagggtga ctagctgcat
    66601 gtgctgatac cccttcaaaa ctaaactcta atgaatctat cgccaaagcc ttacagttta
    66661 tattattaaa agcttctata tgcatttgat aacttacatc aacatcatca aaagctcctg
    66721 ccttaactaa atcacatttt cctcctgttg tttcttcagc tggtgttcct attagtacaa
    66781 tttttccttt aaaattttct tttaacttac ttaatactat acaagctcct agtgtagatg
    66841 ctgcaatcca gttatgtcca caagcatgag ctggttcttt attttctcca tatccaggaa
    66901 gtgcatcata ctcagctaaa aatgctatgg taggtccatc attatctcct aattcagctc
    66961 taaaagcagt ctccatatta caatatggat aaactgtatc aaatccatat tccttcattt
    67021 tttctactaa gtattttgat gatatgtatt cttcttcccc taattcagga ttgttaaata
    67081 taaaattaca aacttcttca atttcttttc caagttcatt ctctatctca taagctctct
    67141 tctttatact ctctatcata atttacctcc tactagaatc ctagatataa tcctattgtc
    67201 aaaaatgcca ttacaagaat tgttaatact accattaaag gaattacgaa tctaacccac
    67261 tttgaccaag gaatattggc tactgctagt gctcccatta aaacacctga tgtaggtgtt
    67321 atcaagttag ttacacctat accaaattga taagctgtaa ctatcatttg tcttgataca
    67381 tctactaagt cgccaagtgg tgcaagtaca ggtattgtaa gtgatgcaag ccctgatgct
    67441 gatggtatta aaaatgctat taatccttct attaagaaag ttaaatttat aaatatagtt
    67501 tttggtaatc catttaataa atttgctgca gaatttaaaa ttgtatctat tataaatcca
    67561 ttttctgcta ttattacaat tccacgtgca aatgctatac acaatcctgc tgtcattaaa
    67621 tctcctgctc cacttataaa tgaattaact atatcatctt gtttaagacc tgatgctata
    67681 cctgatatta tacctatagc tgtaaatatc attgcaattt cagttatata ccatcccaac
    67741 tttaaaactc catatatcat aatcgccata cctattgtga aaattgctat taccatggct
    67801 tctctttttg taaattcctt tatatttgta tcactattaa ccattgattc ttgatttagt
    67861 gatatatcgt gtactaaaga ttttttaggg tcttttttaa ctttattggc atacatcata
    67921 acaaatccta tagatatagc catatatatt ataaacataa caactctaaa ctctattcca
    67981 gaacctggtg ctatttgtga taatgcttgt gctattccta cagagaaagg attcgtagta
    68041 gctgctccaa atccagctgt tgctcctata aatactgtgg ccactgctgt caatgaatca
    68101 tatcccatac tggtcataag tggaataaat atcatataaa aaggtagtgt ctcttcactc
    68161 attccaaacg tcgtcccacc tagtccaaat agaatcattg aaatcggtat tattaaaaat
    68221 tgaaacttct tcattttatt tacagtatga gctataacag cttctatagc tcctgtttta
    68281 ttaactatcc caaaagctcc tccaactatt aaaacaaatc caactacttc agcagcatct
    68341 ataaacccgt ttataggtgc tgtaaataca tcagtaattc cttgtggatt tgatggtgct
    68401 ttttcataag ttccagctac aacaactgac cttccgtcta tatctactct ttcaaaattt
    68461 ccacttggca ctatccaagt aagaattgcc attaacacta ttagcaaaaa tattattgta
    68521 agtgtctgtg gcatagtaaa ctttttcttt tttaaactgc tcatttttaa tctccccact
    68581 ttttaaatta aatattttat tattgcatcc ctttatccta ttttgtatag atgtaaaaaa
    68641 gctcaacatg gatacatgtt gagctaaatg tgctaaatac aacatacctg catcttccct
    68701 ttatcttgtt taagatagct ctccatcaaa attcgggata attttgatga cagtactgta
    68761 cctattaaat acagtcccag cactagcttc ggacactagc acttcggcac ttactccttt
    68821 tatattgcct cattggtttc caacctcttc aatattaatc ttagtaaatg caacctctac
    68881 ctcaatctgt ttgttagatg aggtaattct tattaatttt tattaattca aagtaattgt
    68941 agcataatat gtcgagattg taaatatttt cagtataata attcttactt tatgatttca
    69001 ttccattttt cacaaataat tttttctcca tataatttaa atactgtagt atattttaaa
    69061 tacacacaaa ctttccacaa attttaaatt attataatac ctttataata aaaaataaaa
    69121 gctatgtaaa cttatcaatt tacacagctt tatatacatt aatctactcc aagtacagct
    69181 ttaagctttg atgctattgt aataactcgt ggaccaaata taacttgaac tgagtttttc
    69241 tttttaacaa ctccacttgc acccaattca tttacccaga agccatcttc atagaatcca
    69301 tcaattgaat ttagctctac cctaagtcta gttgcacagt ttgtaacatt ttttatatta
    69361 ttacttcctc ctaaaccttc tattatagaa tgtacaagct ttgtatcttt atcatctact
    69421 tcctcttcta ctgcattgtt gtatgctgtt ttaccagcat ctacagattg tctttctcta
    69481 tattctttct tactataaag cttaacttct tctgaacttc ttcctggtgt ttttatatca
    69541 aacttcttga ttgcccatgt aaatattata taagtaacta taaaaaatat tggtatcaaa
    69601 aataataatg gtaacgcatc cactttttgt ggttgtaata aattaggaaa catatttcta
    69661 aatgattctc ccattataga aacattaaac atttctgtaa atacatacgt caatcctgcc
    69721 agtggtacat atgctacaaa atacaataat ggtgcaacaa ataggaatgt atactctatt
    69781 ggttccgtta cccctaccaa catggctgtt actattgctg gtatcaatat acttattgct
    69841 tttttcttat tagcattatc agccaactta tacatagcta aagctgctgc tggtaatatt
    69901 gcaaaatgta ttaataaacg acctgttgtg aagttacgag ttatataaga tagtgtatct
    69961 gggtctccca tttgagctaa tcttatatta ttaacaccct cgtaaattgc tccacctact
    70021 tccattgttc cacctactct tgtatattct atagggaatg caattaagtg atgtatacca
    70081 aatggtaata atgctttatc cattgctcca aaagtaaatg ttcctaataa tccactagaa
    70141 cctatccaat taccaagttt agataatgcc aaccctactg gtggccatat aaagtacatt
    70201 gctgtaccca atactattga tgctataaac atcataatca ttgaaaatct tggacctgag
    70261 aaaaaagata atgcactagg aagttctatg tcaacaaatt tgttatgtac tgcactagct
    70321 acaaatccag ctattatagc tccaaatata ctacaatcat atgaaaagat tcctaagaaa
    70381 gaaccatata aagaacttcg actatatgct tctgcctctc ctaatccaga tgccataaac
    70441 gcttctgctg taactgtatc tgaatttata ccatttgctg ctaacaatgt acttataact
    70501 gtgtgcattc ctataaataa aacaattcct cctaatgcac cccatccttt ttccttttta
    70561 gataatccaa aggctatacc tgtaacaaag aatattggta agtatctcat taccataaat
    70621 cctaaatcgt ttattacttt aaagaataca tttataaatg acccttcttt tattagtccg
    70681 ctaagcccta atgcagaaac gttttccata tttgcaaaag ctgaccctag tcctataaat
    70741 actcctgcta caacaagtaa tataacaggt atcatcattg ctccagaaag tacttgtaat
    70801 ttatccttca tagtattatt tcctccattt ttattgaaat gccaggctat ataatgcata
    70861 tatatatact tctattgctt tgtataatgt atctatactt atattttcat ttgcctgatg
    70921 tgaatttgga ttatccccct ctattgtggg tccaaatgca actccatttt ttaacactct
    70981 tgcataagat gcagctcctt ttgtaaaaag ttcggcttct tttccaaaac cttgtttata
    71041 tgcatttgat aatttattta ttaattcact atctggtttt acatacagta attttaaatc
    71101 cttatatata tcaactatta aattttcttt ttttccaatt tcattaattc tattagttat
    71161 aaactcccaa cttataccct taggatatct aaagtctatt ttcatattca attcattttt
    71221 ctctaagaag atactcatta tacatattgt agaaactccc atatctacat cttctttata
    71281 tatccctaat ttttttccat gattatcatc tgtaaagtat gaatttaaaa tatcccttat
    71341 tataacgcct ttatcattta gttttttaat tttctctaaa tcttttgcta aactaaatgc
    71401 acagttatat gacctgtgtg gatttctcga taaagccctt tctcctgtat atcttactaa
    71461 tactttacct tcttctagtt cttctatctc tgtataataa gctaatgatt ctactaaatc
    71521 tttcttatca ttagttttaa aaacagcctc aattttgtca catacaaatc catcttcttt
    71581 gtttgacttt atatcaacta aattgtgatt tctaaattta tctttatcta ttttctttct
    71641 tagattgaag tataaaatac ctttttctcc atttactatt gggaagtttc catctggaga
    71701 aaatgcaaat tttggctgtt cattgacact aaagtaatgc tccatacatt cccaagtcgt
    71761 ctcttcagct ccacccacta tcaatctaat ctttctcttc ggcttttcat taagttctct
    71821 taagaaaagt aatgcatata aaatccctat caaaggtcct ttgttgtcat taactcctct
    71881 accataaaga tagttatctt tttgacatac tttaaaaggt ttactcttcc aaagttcttt
    71941 attatatatt ggcaccacat ctatatgatt aagcacccct actacttctt ctccttctcc
    72001 atattctata tgaattgcat atccatcaaa gtcttttaca acaaaatctt tgtcttttgc
    72061 tatttgaata agcttatcga aggcatttct tatttctatc ccaaatggtt gatttatatc
    72121 tgttgtactt tcatctctaa ttgaaggtat ttcaactaaa cttctaatat catttatgat
    72181 attttcttga ttggatttta aataatcaat aattagttct ttgtttacta tcatcttaat
    72241 tatttatcac ccctttatat taattttaca aagcatttca gttcatatct acattataaa
    72301 ttttgaatat tattttttca atatttcaaa tacttctcta agttaccata atgatacggt
    72361 aaacaatcaa cagattatga caaaatcata ctatacctat tactaatatt ttaacttatc
    72421 tattacgtct tttttaatgt aagtttattc caaaaataca taaaaaaaga tgtagcctta
    72481 gctacacctt cttatatcta ttcaacttcc attattatgc tattaatatc tacatttcca
    72541 ggttctatcc tatcttttac tgttctctta acagaatcag catcatccat tataactaca
    72601 ggagttatta tatttaaatc atttgacgtt ataaaatcta aatctacttc cattattaaa
    72661 tctttagctt ttaccttatc tcctgccttt accttaacat caaacggctt tccttctagt
    72721 gacattgtct ctaaacctac atgtattaat atatttgtat tatcattact cttaatcatt
    72781 attgcatgct ttgtatcaaa tacattgact atttcaccat caacaggaga atataactta
    72841 ccttcacttg gtattattgc tactccatct cccatcatct tttgggcaaa aactgcatct
    72901 ggaacatctt ctaagttcac taaatttccc ttaaaagggc tatatatttt ctcaatctta
    72961 ttttcttttt tcttaaatat attaaacata acacactctc cacattataa aattttatga
    73021 taatattttt cttaaagctt ttgctacacc atcatttaca ttagtatcag ttacaaaatc
    73081 tgccatagat tttacttcat ctgtagcatt tcccatagca ataccaagac ctgcaaattt
    73141 aatcatggat atatcatttt cattatcacc tatacaaata acttctttgc tatctatacc
    73201 taatatatca caaaattttt taactgcaat accttttgaa gtacctttac tcataacttc
    73261 aaagtttatc tttccagaac ttactacttc taaatcttct tgttttttaa attcttcctt
    73321 tgcttctctc aaagaatcta cgttttctga gaaaagtata cattttaaaa tttcattatc
    73381 agctttttct aataaagttt cacaacttgc attttctatt atttcaattc tgtcttcttc
    73441 tttattttta ctatttatta atctatatgg atgttcttca cctatttcat tgttggatat
    73501 aactatttta cagcctttaa aatgtttgct taaattatac ttttcaccta ttgtatagat
    73561 tttctttaga gcttctttgc tcaaaacctt cttatactca tatcccagta atttaaaata
    73621 tgtcccatta gttgatataa tatatatatc atcccctaaa acacttgtaa aatatttcat
    73681 agcattataa ggtcttcccg taactaaggc taccttgata cccttttcca tgacttcttt
    73741 tagtgcttta atattttctt ctgatacttc aaatccttta cccatcaaag taccatccat
    73801 atcagtacaa attaatttat atcgcatttg tatcacatcc tagtaagatt tttctaatat
    73861 ttcaacaagg tcttctttct ttccaaaagg taaaaatcct aaaactggac agcttgttat
    73921 ttgttctaat tgttcctttg ttacattaaa ctcagacatt tttgtcttaa gtcccactct
    73981 ctttaaaaaa ctttctattt catttcctaa agttttagct gcttcatcat cttccacttt
    74041 ttctaattct tggtttagca ttctcgctac tttggcaaat ttctctatat tatccttata
    74101 gaacttttct gcaaattttg ggaacactat agccaaagcc tcaccatgag gaatatgtgt
    74161 aactcctcct attatttcac ttagtggatg tggtgctgct gctccagcat ttgccagtga
    74221 acttcctcct aatgtatctg ctactgctaa ctttgttctg tattctatat tatttttatc
    74281 ttccattact ttaggtagat agttaatgac taattccata gatttttcag acattatctc
    74341 tgtaaaatca ttagcatttg gatttatata actttcaaat gcatgagtaa aagcatcaaa
    74401 tgctgttgat gcagtaattc tctctggcag agttaacata agctctgggt caactataca
    74461 ttcttttgag aaacaatttt gatgaaatat tgtatttttc tcgcttcctc tcgatattac
    74521 tgctgcttgt gtaacttggc ttcctgttcc agaagtagtt gatacagcta ttaatggtaa
    74581 ttctttttca cttaccttat cgtatatttt aaatggattt gtatatgtac taaacatata
    74641 atcccaatct atcttttcta aaccaaaagt caatgctaat acttttgcag tatctatact
    74701 tgaacctcct ccaacagcta aaacaacatc tacagattct tttttagcta attcaaaccc
    74761 ttcatttatt aattctacag ttgggtttgg ctctactcta tcaaagtgta caacttctat
    74821 acctttttct attaaattca atgtaactct tttatataac ttgtctaatg gctctacatt
    74881 tggagtagta actaacaaac atttctttcc atattttcct attattctag gtaattcact
    74941 taaagttcct tgtccaaaat ttattcttga tggttgataa aagttaatca tatttttcct
    75001 cccttaaatt attgccttca tattcaaatt atacatatca taggattttt aaggcaatac
    75061 cttcactgtt aactcttatt tactaaagct tatggcaatt attagacaat caatgtttta
    75121 ttattaccat atcattatgt aaattttatt aagtacccat gttataattt tataatcaat
    75181 cttattgtat aattgactta ataataaaaa tagagtatat acactttttt atgacaaata
    75241 gcttaaagga gaattaaatt atgaaatatt tgatagacag tgctaatctt gatgaaattc
    75301 aaaagtgttt aaagtatgga tttaagggaa taacagctaa tccatctatg tatcttaaag
    75361 agaaagtaaa cttccatgaa tttattgaga catgtgctaa tttaaatcca gaaatattaa
    75421 ctgctgaagt cattggaaat aatttatacg aattaattaa ttcatgtgat aagatattaa
    75481 atataaataa aaacactata ataaaaataa atttctctga agttggctta gaacttataa
    75541 acatattaaa tagtaaaggt gtaaaaactg catgtacctt gattttcaat gtaaatcaag
    75601 catcattagc tataaatgca ggtgctgatt acctattccc ttttataggt agaaatgatg
    75661 aaaatgggaa tgatggtata aaaaacctta cagaaatatg taaccttata tcaataaatg
    75721 gatataatac taaagttgtt gcagcttcta ttaaaaatgt attccactta actcaagcat
    75781 ctctttgtgg atgtgattat gcagctgtaa catatgattt atttaaaaaa gcttctttta
    75841 accagcttac aatagatggt gccaatactt ttgaaagaga ttggtccaag gttaataaat
    75901 agtaactaag atttaggagg gtttgcattg aacaaaagat tattagatat tgctgaatta
    75961 ttactcaaca gctcagacta tattactgta gatactattg ccgaagaatt aaaagttagt
    76021 aataaaacta ttcgtaatga ccttgttatt ttagatgaat ggcttttaga gtttaattta
    76081 tccctagata aaaaaactgg ttcaggggtt attattctag gtaatgaaga tataaaactt
    76141 aaagttatta gagatatcaa tgataaatca aattgtatat atgcatactc tcctgaagat
    76201 agaaaaaggt atattctaag taaattattt attaaaaatt ctaagtttag gattcgtgat
    76261 atttgcaatg aactacatgt tagtagagct actgtgcata aagacttggt agttattcaa
    76321 aattttttgc aaaactttaa agtgactcta gttagaaaaa ccaataatgg agtatattta
    76381 gagggaaaag agaaagatat tcgaaaagca atttttgaac ttgctacggt taataaaagt
    76441 tatactgaac ttaaagaaat attattctcg aaatctaatg aatgcaaaga taccccttca
    76501 acaaaaatat ttaaggagct atttaactat gattttgaga aattatctca aatcactcta
    76561 cacaccttac aagtagaaaa aagtaacctt agtgatgagt attatataaa cttcttaata
    76621 catatagcta tatgtattaa aagaatttca attaaaagat atattaactt atctgaaggt
    76681 ttttttagag aattattaga acatgaatac tttgaaaaat caaaaatttt atgtgctaac
    76741 ctaagtgaag cttttgacgt agaatttatt gatgaagagg tctgttatat ccttcttcat
    76801 atagaaggat tacttaaatc aataaagaaa actactactc ttgaacctct taaaaatgaa
    76861 gaaattacag agtataatct atctgaagca atagctgttc attggggaag tatactaaat
    76921 ttaaacttaa aggatgattt aatattaata aagtctcttg ctaatcacct taaatcagtt
    76981 ctacacagaa taaactatgg gtttactatt acaaatccca tattaaatga tataaagaga
    77041 acatttcctt atacttataa ggctgctaaa gagtctaata ctgttatcaa acagttgatg
    77101 aattatgaag tcaatgatga cgaaatagga tatcttgctt tatatttaat atcagcaata
    77161 gatagaagca aatctcccct taatactatt ttaatatgtc actgtagtga tggtgtatct
    77221 aatttattag tccaaaagtt aagttttgaa tttaatcaga ttaatatagt taaatctata
    77281 cctctatctt ctatttcatt tacgaacttt gatgatgtgg acttaatact aaccactgct
    77341 cctgtagact ttgaacataa tgctgaactg ataaatataa atgctctttt aagtaagaat
    77401 gatatttcta gacttagtac aattataaaa aaactatatt ctgaaaaaaa taaaatatta
    77461 tcttataaat aaacaaaaat aggcttgtct caacatagag ataaacctat ttttcattgt
    77521 ctaaatgata aaaatatatt tgatttcata gaaaaagtgc ctcatgaact aaaaaattca
    77581 ttttgagaca cctccatttt ttatatttaa gctttttatt taagctttta tattttttaa
    77641 acttttatat ttaaagtaaa ttttattatt tttattgagc acaagcatta tttatattat
    77701 taatttcttc agtattctta ccaaactttg tagttacaaa tcctattgtt tttattatga
    77761 gtaataaaga tatcaatgaa tatatagata tcataaaatt cttacttatt attccagacg
    77821 ctactattat aaatccatct agcataaata atataactgg caacttcaga aacttaatta
    77881 ccttattaat aagcattgca actaaatcag ttccaccagt actacagccc cttattattg
    77941 ctatccctat agcagttcct accaataacc cacctatcac tcctgctact aatagattgt
    78001 cagttatgtg agtttgtggt attactttta agaataatgg gcccataaga gttatatata
    78061 atgtttttat tccaaaacct ttccctaaaa ataataatcc cattactaaa agtggcatag
    78121 atattcctaa tgatatattc tcaacaggca ttgataagaa tgtgctaagt acgactgata
    78181 atcctgttat ccctcctgga gctaatgtat gttctgcaaa gaaaaaatta actgctaatg
    78241 ccattaaaaa tgctgatatt gttatcagta aataatcttt tatcatttct tttttcattt
    78301 aataacctcc tatgattata ttatttcagt ataaaaaatc aattaaatta gtctagcttc
    78361 ctgtagtttt tcataaaatt aatgcttcct taaaacatta agaaagcatc tcttttatca
    78421 tagtttatta tcttattcta aaccaaactt tgtagctgct ttatctatag cttttctaca
    78481 attcttaaca cctgctgtta taccatcttt atccttgaat attgctgaag ataatattac
    78541 aacgtctgga ttacattcta ttaatggctc catattgtct gctcttagtc caccatctat
    78601 tgctaattca cacttaggat tcttttcgtc tatcattttt ctagctcttt ttactaaatc
    78661 aacagcactc tttctccatc cccagttatc ttttccatca gtttcatcaa caccatgaac
    78721 gactatttgc aatctatcta tatcatatat agcttcatca acaaaacata atggtgtgta
    78781 acatccaatt gttaatccta ctttcatacc aaattctcta cagtaattta ttatgtatgc
    78841 aagaggagca cctaaaaaat gttctgctgg tattatcaac aagttacatc ctgctgctgc
    78901 tattttttct ataaataatc tatcacaatc tcttgtgtat atatggcatt ctattggttt
    78961 atctgtcact ggtcttattg cttctattat ttgatgacca cccataagtt gcatattttt
    79021 taagtcatgc atatctgctg catctgaatg cacgtagtct acacctgcat ccattgcttc
    79081 tttaactacg gctgatatat gaccgtaatt tacatgtgct aatcctgctg ctatttttat
    79141 gtgtttcata tttgtatacc tccatatttt actttaatta gtattaaata ttagtttttg
    79201 atattttttt aatatttttt aatccagtat tacttttatt tagcattact tttattgctg
    79261 tatatatttc ttttatgaac ttattctgct tttgttaact gtgaacttat tttgctttgt
    79321 aaattaatat tacactgata tatttttttt cacaacactt tgactactcc ttaaaagtac
    79381 ctttatattg tggtaaatta tagacattaa ttgtatataa aaagttagtt ttttaccaca
    79441 ttcttatggc atttattgtc tatactaact caattgatat agctagttca tttaatatat
    79501 aatcagacta taaatataaa tttactcgat aaaaagtacc attcataaca atattaatga
    79561 aagggagatg taaaatgaga aactgtatta ttgcccaatc tggaggccct acagctgtta
    79621 tcaatgcttc tgcagttggt atttatgaaa aaaatttaga aactaaatac tttgataaag
    79681 tatattttgg tatcaatggg attgaaggta tattaaacaa aaatataata gattcagact
    79741 ccttaaacaa agataatgta ttaaatttaa agcaaacacc ttcgtctggt cttggttctt
    79801 gtagatacaa actggctgat tataaaataa attcttatga ttatgataga gttataaata
    79861 tactagatga atatgaaata gatacattat tttatatagg cggaaatgat tctatggaca
    79921 cagtgatgaa attttctcaa tactgcaaaa acactacttg taaaataaaa tttataggta
    79981 ttcctaaaac aatagataat gatttattag gaactgacaa ctgtcctgga tttggttctg
    80041 ctgcaaagct tatttctaca ctaataatgg aaacatactt agatttttct atatacaaaa
    80101 ataatggtat atttatagta gaaactatgg gtagagatac tggatggtta gcagcttctt
    80161 cttcactagc tagacttaat gatactccac ttgttgattt aatatacctt ccagaaattg
    80221 atttctatga agataagttt atagaagatg ttagaaaaat atttaaagaa aaaaataagg
    80281 cttatatagt tgcgtcagaa ggtataaggg ataaatacgg caactttata tgcgaacaaa
    80341 aaacttctgc tcatgataaa tttggtcatt cccaattagg tggagtaagc aactatctaa
    80401 agaatcttat agtatcatca tcaattacaa caagagtaaa aaccttagaa cttggtgtaa
    80461 tacaaagatg tgctatgcat ttagcttcag atactgattt aaaacaagca gctgaagttg
    80521 gtgcatatgg gatagaacta tctaaaaaag gatatacagg agttatggct atcattaata
    80581 gaacatgcaa taatccttat aagtatgaag ttagtcatac tgatatttct aaaatagcta
    80641 acaagacaaa atattttcct aaagagtgga taaatgaaga atgtaattat ataactgatg
    80701 aagccctaaa ttatctatat ccacttattc aaggtaaacc agatatcaaa tataaaaatg
    80761 gactacctta tttttcaatt atttactagg aaattattat aatttgatat acactcaatt
    80821 ctatcgctct taaataccta agtggctgtt aaataaaatt ataaattgtt atatgacagt
    80881 cacttaatcg tttaattatt taactgttta gttttttaat tttttaatct tttttaatac
    80941 taatacgatt tgttgtaata atatctcaca cccattttca ttttggatag attataatta
    81001 gttagacaat aaatattttg tcatgatatt attatatata gtgatacaaa tagaaatact
    81061 tttatcatgg ctagaaaaac aaagattaca caaaggattt aaaaatcaaa caacttcaat
    81121 taaaataaat ggttaaaata acttctaaat caactactat aaacaataaa agaacataaa
    81181 gtagttaagt ctacaattat aaaattagta agactataat aacttaaata aaattaaagt
    81241 taacaaagac tattaaagtt agcaaatatt cctctaccta aataattgct ttttataaag
    81301 agttatttat tttaattgaa tttaattgaa atacgatttt aactagaaca atagattaat
    81361 gtaatattta atataaaata attataataa ttgaaaagga agtaatctat gatgaaaata
    81421 gcagtcataa caaccgaatt tttaaaagag tttgtagata attctataaa aaagttaaat
    81481 attaatgcag aaattgaaat atatatctat agagattttt ctcatgttgg agatttatat
    81541 ttagaaatag aagataggtt tgatggattt gcagttagtg gccccatccc caaaaaagct
    81601 attacaaaaa aggctggaac tattaaaaaa cctctagttg attttggtac tgacttacaa
    81661 agttactatg atatgtttct aaagcttatc tttaaatata atacattaga tttttctaga
    81721 gcgtactttg acttaacaga ctgggcagat aatccaaaag atatatcata ttatttatca
    81781 aatggcacat ttggagactt aatggatagt attgatgaaa atgcatctaa aatgagttta
    81841 tctgacatag caaaacttga aaaaaagatt gcaaaaaaac atattaatct atggaatgaa
    81901 gggaaaatcg atttttctac tactcgtttt agtagtattg ttcctatgtt acaaaatgca
    81961 ggagtaaact ttcatttcat ataccctgat atagatttat taaaaaatac ttttaataac
    82021 ttaataaaag atattcattt acatagaatg aagggaaatc aaccaagtgt aatatatata
    82081 acagttaaag atacaaatta tgatgactct actctagacc aagtttataa aatattaaat
    82141 agtattaaaa aagataaatt aaccgatttt ataattgaaa aaaatagtaa ttcttttaaa
    82201 attttcacta aatgtgaaac gattaaatca ttaactaaaa attttactgc atgttatata
    82261 aaagaatcta ttgaaaatag tacaaatatt aaagtatgtg ttggatatgg aattggtaat
    82321 gatataaagc aagctatgtc tagtgccata agtgctaata aggaatctaa aataaatcat
    82381 ttaaattaca gtttcttagt caacgaaaat tatgagacaa taggaccttt actgtgtgat
    82441 aagttactta ctgtttcaaa taaagtaact ccacatataa atacaattgc caaaagttct
    82501 aaattgtcta ctcttactat tcaaaagatt atatctgtta ctgaaattct acaaagagat
    82561 gaagttactc ctaaggaatt atcttcatgt ttaaatgtaa cagttagaac tgctaataga
    82621 tttctaagta gtctcttaaa gtctggaaac gctactatat tatatgaaaa acaaaactca
    82681 acaaaaggta gacctgaaag agtatacaaa ttatttatat cagaaaataa atattaaaat
    82741 ccaaaatgca taaaaatatt ttatcatcta atattaagta aaattatata tgactttcta
    82801 aaaggtatat ctttgcatct ttttaagata taccttttat aatatttaat aatattttat
    82861 gttattatct actaaaatta ttttctaacc attttactgc acaatgagca aataaagctg
    82921 aaccataaac taatccctct tcattaaaaa ttgctttagg attatgcata ccaaaattat
    82981 atccctgact aatacttccc attccaatcc ataaaaatac tcctggaacc cttagtaaaa
    83041 tctctgaaaa atcttctgaa cccattgcag atggaaagtc tattacattt tcatttccta
    83101 taacttcttt aacataagat acaatctcat ctcctacatc caaatcattt ataactgcag
    83161 gagtccccat tttaaattcc actttagcat ttcctccaaa tgatttagct attcctgtag
    83221 atatttcaat aattctattt tttaattttt ctctaacaga ttggtcaaat gttcttaaag
    83281 tacctgacat agatgcttta tttggtatag catttggtac gcttcctgca ttaaacatac
    83341 caatagttaa aactacaggc tctttctgag gtttttctct tgaaagtata gtgtttaatg
    83401 ctatttgtat atgtgacatt atgtttattg ggtccactcc aaattcaggg cttgctccat
    83461 gagttgcttt tccttctact gttatttcaa atatatctgc agatgccata gctggaccac
    83521 gtgaataagc tacttgacct gtgttaagaa atttacccac catactgtga aatgccatag
    83581 ctacatcgac ctgaggattt tctaatatac catcatcaat cattgatttt gcacctctgg
    83641 tagtctcttc atctggttca aatacaaact ttactctacc tttaagtaag tgcttattat
    83701 ccataagtat tttagctgct cctaagagca ttgctgtgtg attatcgtgt ccacaactat
    83761 gagatgcatc tttattctta cttttaaaag gtaaatccgt ctcctccata ataggaagtg
    83821 catccatatc tgcccttaat aataaggtct ttccaccatt tttactgtct aaaacagcta
    83881 ctattccatt attagacgtc aatttcggag aatatcccat tttttctaaa gtactggtaa
    83941 caaaggttac tgttttcggt agattgcaac caatttcggg attttcatgt aaatatcttc
    84001 tccatctaat aatttcccct tctattgact gcgcttcttt aaaaaaattt ttcatattcc
    84061 ttcctcctaa tcagatagtc agcaaaatct cactttcttt tagtaatgaa atgatgctga
    84121 tataaaaatt ataattttat atattaaatt tcatgtcttt ttaaggagtt ggaatatcat
    84181 ctggaatagg acatttatat gttttaccat tcatagattt atcaaattct tcttttgcct
    84241 ttttagctat ctctggatta tttaatattt tagctccaaa catagccata atctttgatg
    84301 catatatcat acctttttct cctatagaac ttcctgcaca agatgtaaac tgccagctat
    84361 gacctggtgc tcctagattc gtacaccctg taaaaaagta tgctgtcgga actatatgca
    84421 ttacatctcc aatatcagta gagccataac tattaaagtt tgtaacattt cctccaccat
    84481 aatatagatg agttccttct ggcaaattat atttacttat catagcctta tattgttgtg
    84541 ggctttgtct atctaattca gctgcaaaat ccaattcctc tcgagtccaa ggctcttgtt
    84601 taatctcatt catacattct gatacaagat ttgctaaaac atgattgttt aaagtattat
    84661 aacaccctcc taaaaactct acttcaactt cagtttcagt catcattgca gctccttttg
    84721 ctacctttac aagtctttca tatgtacttt ctacagcttc tctacttaaa gctcttgtat
    84781 aataccatac tgatgcttta tcaggaacta tgttgggtgc tactcctcca tctgtaattg
    84841 tataatgaat ccttacatca gaagtaacat gttctctcaa ataattagct cccacattag
    84901 taagttccac agcatctagt gcacttcttc cattttgagg gtctgagcct gcatgtgcag
    84961 tctttccctt aaagtgaaac tttacagagt taacagctgt tgaagtacca attgtagctt
    85021 cactaatttt atttggatga aaatttatag ctaagtctaa cccttcaaac gctccacctc
    85081 tagccataaa tcctttacct gttaattgtt cttctcctgg acatgcataa aatactatag
    85141 tcccacttaa gttattttct atcatctctc ttttaagccc tactactgcc cctacatgtg
    85201 ctgtacataa taaattatgc ccacatccat gaccatatgc acccaattca aatgcttctt
    85261 gttttgttga aactttttga tttaaaccag gtaatgcatc aaattctccc ataaaaccta
    85321 taactggttt acctgaacca aaactagctc taatagccgt ggctaaacct cctacaccta
    85381 cttccacatc aaatccttca cttcttagaa agtttgctgt attttcacat gctttaaatt
    85441 ctttaaatgc tttttctgga ttttcccata ttttcttact taaatcatca agtttgaatc
    85501 tttttttatc tatctcattt atagatatac ttcctatcat tttctattcc tcctataaat
    85561 ctatttacta tcaaccaaat ataattggac ctattatact agcaaatgcc actgatgcaa
    85621 tggttacagt aacaaatcct cctataatca tctttggtaa taaataatct gttgcccttt
    85681 gtttttcttc ttcagttcct tccatagcat caataccttc attggaaatt atctgtgttg
    85741 caggataagc tagcatacaa gttacaccaa ttgcagctga cattcttgga gaatatccca
    85801 atagttttcc tacaattgca gtaaatagta ttataaatac agagcctaca acaagtattc
    85861 ctatagttgg aataatcatc ccaaacaatg ctgttggagt taccttagca agtcctccag
    85921 gtaacatcaa catacaacca aacattataa ttccattaga ttgagattta tctaaaattc
    85981 ctttttctaa aaaacctatt cgtgtaaaaa tcaaccctac taacaaacat gctatgttgg
    86041 gatttaaata atagtttgca ggggtaaatc ctggtattaa agtagccaat cctacaaaat
    86101 taccaatcat agcaacaaat gaaagctttg caagatatac taaagtagat gattttttac
    86161 ctaaatcttc tttaaaaatt ctcatacttg gaagtttaaa attacttttt gactcatctt
    86221 tcaaaaaatc cccgttatct cttttaaatt ttaattcttt atttatacaa tatgtagtta
    86281 tcggaatgcc aaagaacttt tgaaatgata atactagaat tacaaatgca gcaagttcag
    86341 gtctatttgc agcttctgca acagttgtta cgataatacc agcaacagta gaacccgcta
    86401 ttggaggtgc tgctattaaa gcatactctc taccaaataa catactaccc actgttaaac
    86461 agccaacccc tattcctaca atacctacta atgcaattac aaccgttttc cattcatgac
    86521 ataagtcttc aagatttata agtgttccta agttagttat taataaagct ataccaaagt
    86581 tgctcattgc agctaatatc cctgatgctc ctgtaatgtc ttttggtaaa atatttgtcc
    86641 aaaatcctag taaaaatata atacatacaa ataacaatgt tgaaattgca ccttttgttt
    86701 tcttagataa aaactcacta aaagcataaa tcattacaca tacagtaaag caagtaagtg
    86761 gattccaaaa attttccatt gttgtttcct cctaaatata ataatttttt atcaataaaa
    86821 caataattat aaaatgtata ttatgcagtt attcttaact acatatggta tagcttgata
    86881 ttttttgatg atattatata cgtcctaaat tattcctaaa atatctaaat aaatataact
    86941 agagttaact ctctaaatat taactatagt tatcaactta ctagatttct ttttgatttt
    87001 tactttctac ataattttac gagttagaat tgtacttcaa ctattctcta tattagtata
    87061 catgttggaa gacttatatt tatttaaata ttagatatag aatatatttt tataaacgtc
    87121 ttaaattatt cttaaaatat ctaaaacgaa ataataatat acccattaaa ccaatattat
    87181 cagaatatac ttcactttaa gtcttcttta tatagaaatt ttatctatta gtgcatcccc
    87241 tcccttgtaa attagactat ttctaagcga atttagatat cttattccta gagactatta
    87301 ctaaaaacaa aaattattta ttatattaaa tcaatgataa gccatacttt aaaattcttt
    87361 ctatacaagt ataatttatc atataatgac atctatacct ttatctttat agtagtccta
    87421 aattattctt aaattgacaa taaaaataat ttcactattt ataaaaatac ttgtttttat
    87481 attgtcaaac tattcttaat atagattata attttaaatc ttttctttgt caatatattc
    87541 tgacattttt ccagaaaatt tataaagtta tatagtgagt aaatcatact atttttatat
    87601 aatgagtaaa ttatactatg aatatgtcta aatatttact taagtataaa aataaaaaat
    87661 gtgtattctc ctttttatag taattttaat tgccattatt tttaagaaga atacacactt
    87721 tactttaaag accctatact ctattttaaa tgctttacta gacactaatt aacattagtt
    87781 cctaacttct atttatctta ttcctaccct actactatat ccgtagaaaa atctaaaact
    87841 tctccattag attttattac ttctttgtac caatcaaatg attttttctt atatctctta
    87901 aaacttcctt tgccttcatc atctaagtca acatagataa atccatatct cttactcatc
    87961 tgacctgtag ttgcactgac taaatcaatt ggtccccaca tcaaatatcc aaaacattca
    88021 acattatctt cttcaattgc agacttcatt tgagatagat gcttacttaa atattcaatt
    88081 ctataatcat catttatagt tccatcttct tcgattttat catatgcccc taaaccattt
    88141 tctgtaatta agataggtaa ttgatacttt ctgtaaatat agttcaaggt aaatcttaaa
    88201 cctatagggt ctattgccca cccccactta ctttgttcca aatatggatt ttgaatccca
    88261 ccaaataaag atttatcacc tgataaattt tcttttggag tagatacact tgaaaaataa
    88321 taattaagtc ctataaaatc taacttacct tctttaaatg cttttttgtc ttcatcagtc
    88381 acttcaatag ttatattatt tttttcatat tcttttaatt tatattgtgg aaacttccca
    88441 ttgcacattg catcaatttg ataataatca cggtcattat ctaaaaatgc atttaacaca
    88501 ttactaggat tacaattgta tggataaatt ggatttagtc caaatacaca tccaatttta
    88561 ttgttgggat tgatttctct agccaacttt gcaaccttta cacccgccaa agtcatatta
    88621 taccctacat ttgccaatgt ttgaacctta ttttctattt cactatactt aatacctgct
    88681 atcatataag taaaaatatc cgagtgctct gtttgagggt caatatggtt catctcatta
    88741 aatgtaaccc agtactttac cttatcatca aatcttctta ttacagtctc actgtacttt
    88801 aaatacaaat ctataagttt tctgttattc catgaaccat atttatgtac taaattcata
    88861 ggtaattcaa aatgatatag tgttacaata ggttctatat tattttctat taatgtatct
    88921 atcagacctt catagtattt aatccctaat tcattaggat tttcatcatc tccatttgga
    88981 aaaattcttg accaatctat agatattctt aatgcactaa atcccatttc tttaaataaa
    89041 gctatatctt ctttatatct attataaaaa tctattccat tatgagatgg ataatctaat
    89101 ttttcttcta ttttatcagt aattactctt ggcgtttcat aagagccctt agttacaaag
    89161 tccataattg caggtccttt gttatcacta tcccatgaac cttcacattg atgggctgca
    89221 atacttcctc cccagaaaaa tgtatcttta atattcatga attttccctc ctaaaattta
    89281 tactattaca gttaataatt cttctgattc atcatttgat gagattacat ctaaataatc
    89341 gctagtattt gtaactacag ttattatagt agaatcaaag ccttcctcta taattttatc
    89401 agattcaaat actgctaaag catctccttt tttaaccctt tgattttgtg ttacaagact
    89461 ttcaaagaat tttccatcta agtttacagt atctatccca atatgaatta atatttctaa
    89521 tccttcatca gtcactatac catatgcatg tttagttgga aatactatag ttatagttcc
    89581 atctacagga gatataacag ttgattttga ttcactaggt ataactccaa cccctttacc
    89641 taatgctcca gaggaaaatg ctttgtcatt aacttcccca agtgcaatta cctgaccttt
    89701 tgcaacattt ccaatactaa tagttcttga attgttctcg ttgattatta gttctaattc
    89761 tttttcatta tcacccttaa cttcttcttt ttcatctgaa ggtattccta gtaaatagga
    89821 agctgctgtt gctgctacaa atgatactac tacacttatt atcataaatg taaagttaga
    89881 tacctctcct ccaacaagat acgttggtaa tgcagctaat ccccaaacca ttccatatgt
    89941 tttaacttga gctaaacctg ctattaaacc acctatacct ccacctatca ttactgctac
    90001 aaatggtctt ctatatttca taaacacacc aaacactgaa ggttctgtaa ctcccataag
    90061 tgcactaaaa cttactgtac caaatagttg tttttgttct ttattcctag tttttaagaa
    90121 atatccaaac attgcaccac aaactgcaat atcagatatt gttgcagcgc ccataaaaat
    90181 tgggtcatac cccaatgttg tcacagaatt taaagatata ggcatagtaa agtttgctgc
    90241 accaaaaact attaagagtg gttgtaaagc tgcatacagt gcaacaacac tccaattacc
    90301 caatgtatca attaaaaaca tacatgcata gtttaatcca tcaccaatcc acataccaat
    90361 aggtccaaat acagttaatg taactgatag agttattatt aaagataaca ctggaactaa
    90421 aaaatagtgt aatgctttag gtaatatttt tttcaaacct tcaattataa ttcccattgc
    90481 ccatactcct aaaagtattg gaataaaact gtttgcataa gttgtagcct gtaaatttat
    90541 accaaataaa cttaatcctt ctacaccatc tattgatgaa gataataacg taactgctaa
    90601 cactactgct atatagggat ttacattaag tctttttgct gatgctattg caattaatac
    90661 tggtaagaag tgaaatgtag cacttcttaa tgtatctaat atttgatatg taggactgtc
    90721 actctgaagt atgccagaca tatttaaaag tgctaatata cttgcaagta taccagctgc
    90781 aagtagtact tcaattactg aagtcatagt ctctgagact accacaaaga tggaattaaa
    90841 tatacctttg atacctctaa ttcccttttt tctttcactt tttatacttg attcacttga
    90901 acttaaacct atcatagaca taaactcgtt atatacatct acaacatgag tacctattat
    90961 aatttgataa gcaacatcat tagaaactac atcaattact ccttttaatt ttttaatttc
    91021 ttctgtttga gcgatactcc tatcatttaa agtaaatctt aaacgagtaa cacaatgcgc
    91081 taccgacttt atattttctt ttccccctat aagttttatt atattttcat ttaaatcttt
    91141 atatttcatc tgattttgcc tccagtcttt gagtcaccct atttatatgc aacattaaat
    91201 aagtttcttc acttatagat atatcgacat taaattgtga ttttatatat tttcttatct
    91261 tttgaacact tccaaatgac tttggataaa gttgacttac ttgtttatat aaatctatag
    91321 aattatcttc atctattgaa gcttcatccg acataattct ttggataaag tactgtagat
    91381 gagtagtaaa tctcatataa tttgttgaag tttcatctag ctctacatta aaatgatatt
    91441 ttattataga aactatatct gctaaagttc tcaactctac aattgtacta tcatgagact
    91501 ctttatttac ctccatattt acaaaatgaa gtgctattga tactgcctca tcatcaggaa
    91561 gattaattcc cttactatca ccaattaatt ttagtgcatg cattccaatt tcaaaatgct
    91621 ttggataaaa tcttttaaca ttccatacca aaggactttt tataaattga ttcttttcac
    91681 atcttttaag taaaaactct aaatggtcta gtaaggttaa gcttagataa tcacttgcct
    91741 ttatatttaa agctttctca ccatattcaa ttatttcatt aataagaact atattattgg
    91801 agtctgagtt ttgtagtaga taactaaagt gttctagcat atcgtatgaa tctagtatat
    91861 atgttctctc tatttcattt tcatttatta cttgtcccac tcttttttta aagccaattc
    91921 ctttaacaaa tacaataatt tcatttttac ctctttttac taaggctaca ttattattta
    91981 aaatctgaat tattttcata tattcctcct ttcaataaaa agaactcaag catatatgta
    92041 tagagtatcc ctctattttt cacatatata cttgagttct gcctgctaat cagtaacata
    92101 ctcaaaatta tgactacgtc gccataatta aaattctttt ttgtttaatt tcatattcta
    92161 tatgttatac tatccatata aatctgtcaa cggtttccta gatatttttt tattctataa
    92221 ctacaagctc tgttatgcga ttatctgaca aggtttatat actaaattga tactatttta
    92281 ccgtcactaa tatttataat attgtctgaa ccattatcta ttttactatt atgaattatc
    92341 atagtaatgc ttttaaatat aatgaactgt atgttaacaa ataaattctt aagatatatt
    92401 aacatataaa gtcttaagtt tagttttatt taatcactat taagatatat taataggtaa
    92461 agcttcaaat ttagtt
//
LOCUS       DAMJWS010000004        84631 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMJWS010000004 DAMJWS010000000
VERSION     DAMJWS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 84631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.004"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(152..1753)
                     /locus_tag="PC399_000276"
     CDS             complement(152..1753)
                     /locus_tag="PC399_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2591155.1"
                     /translation="MIYDLILKNGFIIGGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIAKQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1864..2541)
                     /locus_tag="PC399_000277"
     CDS             complement(1864..2541)
                     /locus_tag="PC399_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591156.1"
                     /translation="MSKVKSIYNEEYLPFKIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTMLIARAM
                     YKDKLSD"
     gene            complement(2554..3249)
                     /locus_tag="PC399_000278"
     CDS             complement(2554..3249)
                     /locus_tag="PC399_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2591157.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3301..4500)
                     /locus_tag="PC399_000279"
     CDS             complement(3301..4500)
                     /locus_tag="PC399_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2591158.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4761..6806)
                     /locus_tag="PC399_000280"
     CDS             complement(4761..6806)
                     /locus_tag="PC399_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2591159.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7217..8026)
                     /locus_tag="PC399_000281"
     CDS             complement(7217..8026)
                     /locus_tag="PC399_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2591160.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQKFLQFLEE"
     gene            complement(8110..8643)
                     /locus_tag="PC399_000282"
     CDS             complement(8110..8643)
                     /locus_tag="PC399_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2591161.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8793..10142)
                     /locus_tag="PC399_000283"
     CDS             complement(8793..10142)
                     /locus_tag="PC399_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2591162.1"
                     /translation="MELKNFKDILFKKALSEGFEEYEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(10154..11536)
                     /locus_tag="PC399_000284"
     CDS             complement(10154..11536)
                     /locus_tag="PC399_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2591163.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            11780..12994
                     /locus_tag="PC399_000285"
     CDS             11780..12994
                     /locus_tag="PC399_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2591164.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFNFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            13171..15084
                     /locus_tag="PC399_000286"
     CDS             13171..15084
                     /locus_tag="PC399_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2591165.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            15355..16329
                     /locus_tag="PC399_000287"
     CDS             15355..16329
                     /locus_tag="PC399_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2591166.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIIALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            16350..16625
                     /locus_tag="PC399_000288"
     CDS             16350..16625
                     /locus_tag="PC399_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2591167.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            16638..17801
                     /locus_tag="PC399_000289"
     CDS             16638..17801
                     /locus_tag="PC399_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2591168.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKTGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            18138..20417
                     /locus_tag="PC399_000290"
     CDS             18138..20417
                     /locus_tag="PC399_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2591169.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(20512..21852)
                     /locus_tag="PC399_000291"
     CDS             complement(20512..21852)
                     /locus_tag="PC399_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2591170.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(21864..22646)
                     /locus_tag="PC399_000292"
     CDS             complement(21864..22646)
                     /locus_tag="PC399_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591171.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(22661..23683)
                     /locus_tag="PC399_000293"
     CDS             complement(22661..23683)
                     /locus_tag="PC399_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2591172.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(23689..24702)
                     /locus_tag="PC399_000294"
     CDS             complement(23689..24702)
                     /locus_tag="PC399_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2591173.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTKFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(24830..25768)
                     /locus_tag="PC399_000295"
     CDS             complement(24830..25768)
                     /locus_tag="PC399_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2591174.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEDKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            26038..26502
                     /locus_tag="PC399_000296"
     CDS             26038..26502
                     /locus_tag="PC399_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591175.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILDGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(26614..26931)
                     /locus_tag="PC399_000297"
     CDS             complement(26614..26931)
                     /locus_tag="PC399_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2591176.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(26944..27675)
                     /gene="chbG"
                     /locus_tag="PC399_000298"
     CDS             complement(26944..27675)
                     /gene="chbG"
                     /locus_tag="PC399_000298"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF2591177.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(27701..29011)
                     /locus_tag="PC399_000299"
     CDS             complement(27701..29011)
                     /locus_tag="PC399_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2591178.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(29108..30421)
                     /locus_tag="PC399_000300"
     CDS             complement(29108..30421)
                     /locus_tag="PC399_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2591179.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(30443..30754)
                     /locus_tag="PC399_000301"
     CDS             complement(30443..30754)
                     /locus_tag="PC399_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2591180.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(30954..31658)
                     /locus_tag="PC399_000302"
     CDS             complement(30954..31658)
                     /locus_tag="PC399_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2591181.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCEYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(31749..33110)
                     /locus_tag="PC399_000303"
     CDS             complement(31749..33110)
                     /locus_tag="PC399_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2591182.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            33609..34763
                     /locus_tag="PC399_000304"
     CDS             33609..34763
                     /locus_tag="PC399_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2591183.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(34845..36563)
                     /locus_tag="PC399_000305"
     CDS             complement(34845..36563)
                     /locus_tag="PC399_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2591184.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLFTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(36568..37557)
                     /locus_tag="PC399_000306"
     CDS             complement(36568..37557)
                     /locus_tag="PC399_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2591185.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(37653..38702)
                     /locus_tag="PC399_000307"
     CDS             complement(37653..38702)
                     /locus_tag="PC399_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2591186.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(38979..39647)
                     /locus_tag="PC399_000308"
     CDS             complement(38979..39647)
                     /locus_tag="PC399_000308"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF2591187.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(39653..41026)
                     /locus_tag="PC399_000309"
     CDS             complement(39653..41026)
                     /locus_tag="PC399_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF2591188.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(41023..42801)
                     /locus_tag="PC399_000310"
     CDS             complement(41023..42801)
                     /locus_tag="PC399_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF2591189.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(42918..43241)
                     /locus_tag="PC399_000311"
     CDS             complement(42918..43241)
                     /locus_tag="PC399_000311"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF2591190.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(43228..44205)
                     /locus_tag="PC399_000312"
     CDS             complement(43228..44205)
                     /locus_tag="PC399_000312"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF2591191.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(44220..44783)
                     /locus_tag="PC399_000313"
     CDS             complement(44220..44783)
                     /locus_tag="PC399_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF2591192.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(44835..45326)
                     /locus_tag="PC399_000314"
     CDS             complement(44835..45326)
                     /locus_tag="PC399_000314"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF2591193.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(45329..47254)
                     /locus_tag="PC399_000315"
     CDS             complement(45329..47254)
                     /locus_tag="PC399_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF2591194.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVIFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(47256..47567)
                     /locus_tag="PC399_000316"
     CDS             complement(47256..47567)
                     /locus_tag="PC399_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591195.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(48197..48472)
                     /locus_tag="PC399_000317"
     CDS             complement(48197..48472)
                     /locus_tag="PC399_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF2591196.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(48587..49996)
                     /locus_tag="PC399_000318"
     CDS             complement(48587..49996)
                     /locus_tag="PC399_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF2591197.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(50367..51029)
                     /locus_tag="PC399_000319"
     CDS             complement(50367..51029)
                     /locus_tag="PC399_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF2591198.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(51283..53502)
                     /locus_tag="PC399_000320"
     CDS             complement(51283..53502)
                     /locus_tag="PC399_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2591199.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFVAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(53778..56432)
                     /gene="adhE"
                     /locus_tag="PC399_000321"
                     /gene_synonym="adhC"
     CDS             complement(53778..56432)
                     /gene="adhE"
                     /locus_tag="PC399_000321"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2591200.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            56936..57136
                     /locus_tag="PC399_000322"
     CDS             56936..57136
                     /locus_tag="PC399_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591201.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(57343..57933)
                     /locus_tag="PC399_000323"
     CDS             complement(57343..57933)
                     /locus_tag="PC399_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF2591202.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(57936..58811)
                     /gene="dpsA"
                     /locus_tag="PC399_000324"
     CDS             complement(57936..58811)
                     /gene="dpsA"
                     /locus_tag="PC399_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF2591203.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(58887..60278)
                     /locus_tag="PC399_000325"
     CDS             complement(58887..60278)
                     /locus_tag="PC399_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2591204.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRIIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(60282..61418)
                     /locus_tag="PC399_000326"
     CDS             complement(60282..61418)
                     /locus_tag="PC399_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF2591205.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(61450..61995)
                     /locus_tag="PC399_000327"
     CDS             complement(61450..61995)
                     /locus_tag="PC399_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF2591206.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            62319..63098
                     /locus_tag="PC399_000328"
     CDS             62319..63098
                     /locus_tag="PC399_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591207.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            complement(63553..63981)
                     /locus_tag="PC399_000329"
     CDS             complement(63553..63981)
                     /locus_tag="PC399_000329"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2591208.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     repeat_region   64042..65508
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=64237..64265
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(65692..65958)
                     /gene="cas2"
                     /locus_tag="PC399_000330"
     CDS             complement(65692..65958)
                     /gene="cas2"
                     /locus_tag="PC399_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2591209.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     gene            complement(65961..66941)
                     /gene="cas1b"
                     /locus_tag="PC399_000331"
     CDS             complement(65961..66941)
                     /gene="cas1b"
                     /locus_tag="PC399_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2591210.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            complement(66944..67495)
                     /gene="cas4"
                     /locus_tag="PC399_000332"
     CDS             complement(66944..67495)
                     /gene="cas4"
                     /locus_tag="PC399_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2591211.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            complement(67510..69831)
                     /locus_tag="PC399_000333"
     CDS             complement(67510..69831)
                     /locus_tag="PC399_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2591212.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTYLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            complement(69842..70543)
                     /gene="cas5b"
                     /locus_tag="PC399_000334"
     CDS             complement(69842..70543)
                     /gene="cas5b"
                     /locus_tag="PC399_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2591213.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            complement(70545..71459)
                     /gene="cas7i"
                     /locus_tag="PC399_000335"
     CDS             complement(70545..71459)
                     /gene="cas7i"
                     /locus_tag="PC399_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2591214.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            complement(71461..73311)
                     /gene="cas8a1"
                     /locus_tag="PC399_000336"
     CDS             complement(71461..73311)
                     /gene="cas8a1"
                     /locus_tag="PC399_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HDF2591215.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNMIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKTPPEEILQIFVEEDLDFESVGYAFVTGLISGKYEGKDKLPNEKEEK"
     gene            complement(73317..74054)
                     /gene="cas6"
                     /locus_tag="PC399_000337"
     CDS             complement(73317..74054)
                     /gene="cas6"
                     /locus_tag="PC399_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2591216.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            complement(74132..75049)
                     /locus_tag="PC399_000338"
     CDS             complement(74132..75049)
                     /locus_tag="PC399_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2591217.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            complement(75328..77244)
                     /locus_tag="PC399_000339"
     CDS             complement(75328..77244)
                     /locus_tag="PC399_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591218.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            complement(77244..77990)
                     /locus_tag="PC399_000340"
     CDS             complement(77244..77990)
                     /locus_tag="PC399_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591219.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            complement(78094..78945)
                     /locus_tag="PC399_000341"
     CDS             complement(78094..78945)
                     /locus_tag="PC399_000341"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2591220.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            complement(79193..80197)
                     /locus_tag="PC399_000342"
     CDS             complement(79193..80197)
                     /locus_tag="PC399_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2591221.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFAP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKGVNSLIKM
                     "
     gene            complement(80207..80899)
                     /locus_tag="PC399_000343"
     CDS             complement(80207..80899)
                     /locus_tag="PC399_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591222.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            complement(81213..82172)
                     /locus_tag="PC399_000344"
     CDS             complement(81213..82172)
                     /locus_tag="PC399_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2591223.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            complement(82205..83083)
                     /locus_tag="PC399_000345"
     CDS             complement(82205..83083)
                     /locus_tag="PC399_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591224.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIRSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            complement(83076..83858)
                     /locus_tag="PC399_000346"
     CDS             complement(83076..83858)
                     /locus_tag="PC399_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591225.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            complement(83859..84488)
                     /locus_tag="PC399_000347"
     CDS             complement(83859..84488)
                     /locus_tag="PC399_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591226.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
BASE COUNT        28291 a        14545 c         9444 g        32351 t
ORIGIN      
        1 actataaagt ataaatatta tctgattatt aaaataaata ttaatactat aaataaaaac
       61 gaagcatgat ttttatttaa ataaatctca aacttcgtct ttacttactt agatatttat
      121 ttactttcta aatcctatta tttttaaaac cttatctttt taatacttca ccacatttat
      181 tctcagtaat ctctccattt tccaacgcaa tttttccatt tacaattaca tattcaacac
      241 cattggctag taattgtggc tttgaaaaat caatatcttc tgacctagaa taactatagt
      301 tatccatgtc aataactgct atatcagcta tcaacccttc tttaagaata cctctatttt
      361 ttattttaaa ttgcctagct ggtaatgaag taactcttct tacaatctct tccaatctaa
      421 caccagactt tctgtacata ttgaagaaaa ttggaaaagc tcctcttctt tgtagttcaa
      481 accatgacat atccccaccc acactaaaca aacaatctga accaaccata acaaatggat
      541 tatcaattat tttatcatca tgtagtttat ctgggaaatc agctctatac attcctccta
      601 aacaaaagat aagttcttcg tctccatcta aaagtaaatc gagtaataac ttgtcttcat
      661 ctattccttt ttgtgtagct atttcacgta ttgatttctt ttccatatta atatctttag
      721 tgtttagaag tattatactc cccctatctc caagtataaa tgcttctttt aatataagtg
      781 ttcttccctc ttcatttcta agagcatcca ttaccccatt tatacctaat tcaaatagac
      841 tagatgatat tgccataata aattgaatta ctcttttttt cttcatacaa tgcccagaac
      901 ttcttggtac tgtgtcaacc attatttcaa caccattata cctagccttt aatatctcat
      961 caaatgcttc tatttcaact ggacttaagt gagatacttg aaccctagca ccactttttt
     1021 gcccaacctc tatggcttct tttactgcat tatatcttcc tatgtaatct ctaatatgag
     1081 atgtatatat accatcatgc tctttaatct gctttgcaat atcaacaagt tcatcaatat
     1141 cagcatactt acttggtata tatgccaatc cagtagatat tccaaaagca ccacttttca
     1201 ttccatcatt tattatatct gtaatttcat ttttttcctt ttctgtagga ggtctatcct
     1261 tagaacctcc cataacactc catcttatag tcccatgccc caacaagaat cccatattga
     1321 cagaaatacc acttttagat ataaggtcac aatattctgt aaaattattc caacatttat
     1381 ttttatctat aagatacttt ttttcttctt caagtaatag accattttta tacatatatt
     1441 catatacatt ttcagaagaa tatggtgtta tagaatgtcc acaattacca tttatagttg
     1501 ttgtaactcc ttgttttaga aacttttcaa atttaccatc tagtattgct actatttctt
     1561 catgaacatg agggtcaata aatccaggtg ttataacttt tccacaacaa tcaatctctt
     1621 tatttgtatt gctatttatc ttagaatcta tttttgctat taagttgtct ttaattgcta
     1681 tatctccata aaaaccagga ttacctgtac cacctattat aaatccattt tttaagatta
     1741 aatcatatat caaaatattc cctccttaaa aaccaatttt taaataatct taatgtaatt
     1801 tttatatttg aaaatcaaat tattatattt catatttgaa aattcactat tatttcattg
     1861 taattagtca ctaagcttat ctttatacat agctcttgca attaacatag taccaaatac
     1921 acatataaga actactagag actgtggtat tataccttgc tttgataata taatagtcaa
     1981 tacagatatt acaattgcta ctaggcctaa ttttttatct tgtaaaaata cttgtccaaa
     2041 aactgaccca aatagagctg gtaaaattaa gtttaatttt tctactactt cagctggaat
     2101 tttagatagt acgcttcctc ctaaaattac ccctatagtt agtatactta tattcactaa
     2161 aatagataca gctattccta ttgtagatat tatcgagcct tcttctgtac ctgattcaac
     2221 ttctgctgcc ttttgagctg ctattgaaca tggtagcctc atattagata tattaccaga
     2281 aagaaaactc atataagttc ctgggatacc taagattggt gaataagaaa ctggttctat
     2341 tatgtaattt ggagcactca tactgacaat tgccattgta cttgctaata atgctgatat
     2401 tggaggcata ataccaaatc caaagctcaa aattattcca ggaactaatg ctgctataat
     2461 ccccaaagat agtgttagtc ttccatacct tatcttaaaa ggtaaatatt cttcattgta
     2521 tatagatttt actttactca taaaactccc cccttatatt actgaagcta ttatcattcc
     2581 gctaaacatt gatattgtaa gcccccactc tcttaaccat tttatatttt tcttcttttc
     2641 aagcatacac aatattacca ttatagccat tccacttata catgcaattg ttccattatc
     2701 aaaacgcaac accctatcag catttagata tgcaaatgaa cccaacattg ctcctagaga
     2761 tattattggt ataaatgatt tcttgccact agatataaca ttggttatct tatctagttt
     2821 atgtccaaat agtaatgtga atattatcca tcctaaagaa cctaatatca tagtccaaac
     2881 tgcatttgta aatgcaagtt ttgtcatttc aggagaacct aaagttatac ctaatgcatc
     2941 tgttccaaat ccggctgaca ttgattcaaa aattacagaa cctataaatg ataatctcat
     3001 ccatgctata ggcccaccca tagttactat aagcgatacc attccagcaa atatagtcat
     3061 tgaaggccca atagctgaaa tagcactact cttaacagct gactttattt gtttatctgt
     3121 tattcccatt gttttaccaa ctttgcaaga ttttatagca aatataaggg attgtaccac
     3181 tactattgat actgctactg tactcgctat ccacaaaagt ggatgattag ctaattttaa
     3241 ataatccatt tataaaacct ccctataaat cctcaaattt tattaatatt tctaatatat
     3301 ctatttccaa acctgtttta gtactctaag tatatttcca cctagtacct tctctatttc
     3361 ctcatcacta tatccatgct ttactaaata tctaacaatg ttttttgaag tttctgttgg
     3421 gttctcaaga ccttttacat attctacttt gggatgttct tttgttcctg ttattttttt
     3481 tatatcaaat ttagcagcta gtactgagtg taattttaca tggtcgccat atactgtatc
     3541 tggtccaaaa gctacatggt ctattcctac taaattttta atatactcaa aatgttccat
     3601 aaatgactct attgaatgtt ctttattctt ctctgttatt gtagtatgag gtgctgcttc
     3661 tacacctata acccctcctt ttttagcaca tgctattaat acttcatctg gagttagtct
     3721 ctttgtgttc cataaacttc ttgccccaac atggcttaat attattgggt ccttgctata
     3781 ttctattaca tcaagtgtag tttgaactcc aacgtgagag caatctattg ccattccaat
     3841 tttattcatt ctttcaacag ctttttttcc gaaatttgta agacctccat ctttctcttc
     3901 ttttagtcct gaacctaatg cattggattc gctatatgtt atacccatta accttatacc
     3961 aaatccatac agtatatcta ttctatcaag ttcattttct attggtgctg caccttctaa
     4021 tgttggtatc aatgctactt taccttcact tttagctctg tatatatctt ctactttttc
     4081 gcactttata acaaaatctt gatgagctaa atcacatagt ctcatgccta aatctattaa
     4141 tatatcatcc catttccacc ctgaatttga tgttatcgta catacaccat ccattaaatt
     4201 atcaaatata gcatcataat atcctcttga taatgcttca taagcacatc tttgtttacc
     4261 ttccctatta tattcaaata tatctctttc taggtgttct ggaaataata ctggatgttc
     4321 atgtaatgat ataaatattt tttcatccac tatactttta accttagctt cttgaatatc
     4381 atctaattca ataaggtatt ctttaactct tttatctcct ttacacatct caatagatga
     4441 aatatctttt cctttttcta aataactata ggcttcatat cctttgtaag ctgacttcat
     4501 aacaacatcc tccaagttct acaatttttt atatttttcc tatgtagcaa ttgatgtgcc
     4561 aaaatataaa ttttaattgt ataataaatc ttgcaaaaaa taaaaacagt agtaaggttg
     4621 atatttacta ctgttttatt aaaaataatc ttcttagatt atttattcaa tatttttgaa
     4681 ttatcttaat cttaaagata attttattta tttctatcca ttatagggat ttttttaggg
     4741 tgttttgcct ttttagtccc ctatatcact cccttaattt ttacatatga tataaagttc
     4801 tttccttcat cagttataaa actaccttgt ttttttactc cagataaaag taaaccatgg
     4861 ctctttaact tatctattct ttttctaatt tggtctacac ttaaaactat atctttttct
     4921 tttaaaatct cttgcagttt atttcttcct aataatatat ttttattatt ccaagtttct
     4981 atactggtca atatacatat actttcctca aagaaattag attgtttgaa atctaagata
     5041 attgaatcga aattttcatt aactaaagta ttgttttttt caaatctaaa ttgttctggc
     5101 aaatgctcgt aatctactct atcttcctca actatagtat caatataata aattaaattt
     5161 ttaagctctc ttacatttcc tggccattca tacaatgtta aagcttgcat tgattcctct
     5221 gtaaaacatt tgttgctatt tatttcatct aaaaaatact tacttataag aggtatatct
     5281 tccttacgtt ctctaagtct aggtatctct atatgtaata cattaatcct ataatacaaa
     5341 tcttctctaa aagaaccttc tttaattttt ttctttaaat ctttatttgt tgcagctata
     5401 attctaacat ctattggtat aattttactt ccgcccattc tcataacttc tttttcctgt
     5461 aatactctta gcaatctctg ctgtacatct aaagatatat cacctatctc atctaaaaaa
     5521 attgttcctg tatgtgctct ttcaaatata cccatttttc caccttttat tgctcctgta
     5581 aaacttcctt catcatagcc aaaaagttca ctttcaataa gtgtatctga caaagaagat
     5641 agattaactg caacaaatgg cttgtttttt cttagagatt cattatgtat tgcctgggca
     5701 aatatttctt ttcctgttcc attttctcca agtatcaaca cagaaaagtc tgtagttgct
     5761 atttttcttg caatattaat tttttctttt attattttac tctcacctac aacactctca
     5821 aatgtatact ttgatacaaa accttttgac tgaagtttat tttgaatctt gtcctcaagc
     5881 acttgaattg cacttatatt tttaatgctt ataatactct ttattcgttc attattttca
     5941 taaacattta ctttattaat tatcagtttt ttgttattta aatttactac ttcatcatta
     6001 acttcatctt cttgaaaaaa tatttttttg acaaccttat cactaaataa gtccataaag
     6061 ttattagata taatttcatt ttggtctatt ccaactagat tagaaaatac tttgttgcaa
     6121 tatataaatt ttcctaattt atcaacagaa gcaatacctt catctataat ctccaaaaaa
     6181 ctcttcatgg ttttattcat agttgtataa tacctatatc cacttactgt atcttcattg
     6241 tatttttcct taataatatg tagtttatct atagatatat ttagttttgt aaagatttct
     6301 ataactgtat ttatatcaat taccttatca cctatgtcaa ttatttgctt tatgttttgt
     6361 ggtatgctgt ttctgcttcc cgtaataatt ccaatctcac atttagattt atcacaacct
     6421 ggataatatg gtattagatt aatatgaatt aatcctagtt ttctaattat cttggcagtt
     6481 tcatctgctg attctttata agcatctata accataacat cagaatcatt ttctatactt
     6541 attatctgat ttatattttc aatatttata gtacgatgta ctattacaat aggtatattt
     6601 ttatcaatat tattatgtat atattccttt atatcattgt ctgaacatac tacaagctca
     6661 tagcttgaca gttcaatatt tatcctattt acatatacca agttatctat attacaaaat
     6721 tctccaaata tattttccaa ttgttttgat atatctatat ttattttgtc atcagttgat
     6781 acaaataaaa tattatgttt tttcatagtc ccacctcctg tttaacgagt atagaaagta
     6841 ataatttggt ctattgtagc actttttaaa aaagttttaa atcttaaaaa tcaaattttg
     6901 taccatttac tattattttc cataataaaa taatgtaagc tatctttttt cacatataat
     6961 ttaatttcta aaacggttag tttgacatta ttggaatata aacaaaaatt taatataaat
     7021 tcgttaatat attaatacat ttttaagaat gacaaggaat aaattataac aaattttagg
     7081 aatatataat aatagggtct cagaacttag ttacaagttc taaaacccta tttgtttagt
     7141 gtaaaaaaca tcataaaatt tagcttaaat cttttcacat aatattactc tttttctaag
     7201 tgataataaa atttatctat tcctctaaaa actgtaaaaa tttctgatta aatagtttaa
     7261 gctcatcata gaatatagca tgaccactat actcaaaagt atataaaatt gaatcttcta
     7321 ttttttcatt cataaaaact gcaaattcat acggacatat ttcatctaat ttcccatgaa
     7381 atataccagt aggcactctt acacatttta aatcattaaa taattcttca tctcttaaag
     7441 atacagcagt tccaatagtg ccaatacctg atgcactaaa accaatatca ttaaaccatc
     7501 ttctaaagct ttctggtgga tttgatgcaa atactttttc gccaaaatcg gttaccattt
     7561 ctggtctatc agtacatgct tgtgctatca atttatttac atcttcacgt gtcataccat
     7621 atggaaattc tggaggtctt tgtacaaatg atggtgcagc agctgcagct aataccaatt
     7681 ttgacactcc atatccatta aataaactca tatacctaag tacgattgct cctcccattg
     7741 aaaaaccaac aagggtaaag tcttttaatc caatagcatg taccacctta taaatatcgt
     7801 ctgctaattg gctataagta tatcctcctg atgttgcatc agatttacca aaacctctta
     7861 aatcaattga aactgttcta tatccgagtt ttggcaatat atttgtttga tattcaaata
     7921 tcttatgtcc taagggccaa ccatgtataa aaagtacagt ttttttagca ctcggattca
     7981 aatcatatac tgctatgttt atatcattca catatacata atacatatca acagcaactc
     8041 ccttcaagaa aatgtatgat ggttatttta tatatatgaa taaaatgatg aataactaaa
     8101 ttttcaaatt tatttacaat acttctctat tgccttttca aataaactta catgaagttt
     8161 ttcatctagg attattctat ttaatatagc ttttatataa gggtcttcaa taagacttat
     8221 atctttataa taattattaa tagccacatg ctccaaatgc aaatctagtt ttaatctgtt
     8281 acaaatagaa tttccataac atacaaaacc accattccaa tatgcaccac aagaacttgt
     8341 agaacctcta tatacaggat ttccacccaa taatcttata gtctttgcta atatctccat
     8401 atgtaacatt tctgttatag atatatttat ccacatttca cttaactctc tatgcgtttc
     8461 atctaaatca aaattatggt atagatattg atttacactc gtaaattcac ttgatactcc
     8521 ggcatagtca tccatcaata attctgcata atacttattt ggacctaaaa cttttatctc
     8581 tggatatggc tcatcacttg aatatccatt tcgcttgtgc atgtcataat gtgcatcact
     8641 cataatatct cctcctttaa atttaataca tgatattaat tgtatgcatc acgacttttt
     8701 catataactt agataataaa atctacttaa ataataaaaa gccatattaa ttaaaattaa
     8761 catgaccttt tatccacaaa attacttttt aattattcat cttttccagc tactgatagc
     8821 tctttaataa caactgaagg tgaacctata ctactcattg gaaattctaa atcttcccct
     8881 atagcttcaa tatctttcaa caggtcaaaa tagtttcctg ctactgtaat ttgctctact
     8941 ggaaatactt tttttccttc tttgatataa aagcctttgg cagctaaaga aaagtctcct
     9001 gttactgaat ttgcacctga atgaagtcct gcaaagctag taaccatcaa tccttcatct
     9061 atatccttta taatttcatc taaagattta tctcctttct ctatataaaa attagttgga
     9121 tatatgctta taggtgatga atatgaagat tttattccat ttcctgtagt ctttacacct
     9181 gccttattag ccgtttttaa gttatgaagt aaagttataa gctttccatt taaaacaact
     9241 tcttttttaa atgtagctac tccttcatca tcaaaaggag tagatgccat accattttct
     9301 aaaagtgggt catcaactat tgtaactata ggagatgcta tcatgtctcc ttctctattt
     9361 ttcaataaag ataatccttt ttgagctgaa tctgcattaa atatcccaga aaatgtgcta
     9421 agcaaagata ccatagcttc attaagcaat atagttttat atttacctga tggaatactc
     9481 ttaccaccta ctttagataa agcattatca acaccaagtt ttgctatctc acaaggtttt
     9541 atttcttcaa tagaatttgc tatattatat cctattccat catatttttg tccattgtct
     9601 tctatgatag gaactacaaa ccctataagc atattagttt tattgcttaa attaagacct
     9661 ttagtattag atataccatt acttgataca gaataagata tagtacattt agataaattt
     9721 accaccttat ctgaataagc tttagtctct ctttccattt ctaaagctaa atcaatcaac
     9781 ttatctgcct ctaaattttc tagctcttta gaataagtct ttacatcatt atagtgctta
     9841 tctccttcat atataaattg tacatcttca ttttcaatag tgtttactcc atcttttaca
     9901 ttttttatca acatatcaac agctttatca tctaaaattt ctgtataaga ataacctatc
     9961 tttccattta ccttacctct aaaagataaa ccaaaagatt tatcaagatt gtatttctca
    10021 acttctcctt catatatatt tatacttaaa ttttctcctg tataataata tatttcatac
    10081 tcttcaaaac cttcacttag agccttttta aataatatat ctttaaaatt tttaagttcc
    10141 acaattatcc ctcctatctt ccacctacag taatttcttt tacccttatc ataggttggc
    10201 ctacattagt tggtatactt cctgatgatg agccacacat cccctgagct tgttttacgt
    10261 tattcccaac catatctata ttcataagta tatcgctacc ttttccaatt aaactagcac
    10321 ctctaactgg ctcttgtatt tctccatttt ttattaagta accttctgaa actgcaaaat
    10381 taaactctcc tgttacagga tttacagaac caccacccat ttttttagca taaagtccat
    10441 ctggtattga ctttataata tcttctggtc tatcttctcc agcagcaata taagtgttag
    10501 tcatccttga agttggtgca aatttgtagt tctgtctacg agaacttcct gtaggattca
    10561 ttcccattcg tctaccatta agtttatcta tcatataaga cttcaaaatt ccattttcaa
    10621 tcagtatatt cttttgagtt ggatttcctt catcatctat attcatagaa ccccagtagt
    10681 ttggtattgt accatcatct atagctgtaa tctttgtgga agcaatctgt tgacctaatt
    10741 tatctgcaaa tacagagttc ccctttgcaa ctgatgtagc ttccaaagaa tgtccacaag
    10801 cttcatgaaa tattactcca ccaaatccat tatctattgc taccatcata ttacctgcag
    10861 gacaattttt agcatgcaac atagtatgcg ctactctagc tgattctttt gcataatatt
    10921 ctgggtctat ttcttcaaat atttcaaacc ctttacaacc cccaggacct tcaaatccag
    10981 tttgattttc attaccctta gatgcaattg aacttatccc tagtctggtt cttattcttc
    11041 tatcctcaac atatatacct tcagtgtttg caattaatat tttttggtct ttatccaaat
    11101 agctaacact tacttgagat atatcattac taaattcttt tgcactctta tatgcaacct
    11161 tcataacact tactttatct ttatttccta ttgaatttgg ataaattttt atattatgta
    11221 tgttatttat tttaatactg tcatttaata ttacactttt tccatcttcc aatttaccta
    11281 aagcaacagc agctttgtat gctacatcca acagactatt taatgaattg tcacttgtat
    11341 aagcatatat acttttcaat cctttaaata ttcttattcc tataccataa tttcttccac
    11401 ctattgcatt ttctacttta ttatctatta aactaataga attatttata gtgtcttctt
    11461 caaaaatctc agcaaaatct cctccagtaa ttagacattt ttctaatacc ttagaagcta
    11521 tattttttga aagcataaat ttacctccta ctttaaattt ttaattaact tttattcaat
    11581 ctttttactt gttaacaaac attatatact atattactta aaataaggta attatgaata
    11641 tattatacat atgttttata ctaaaatgtt atcacatttt atctatacat acccatataa
    11701 ctagataatt aaaatgtata atattatttg ctatagaaaa taatacactc atacataata
    11761 aattttagga gtgaagtata tgaatcaaga atatgtaaaa ggaaatccta tttttaccaa
    11821 tattaataaa gttccaagac aatttccata ccttactgat gatattgata ctgatgttat
    11881 aattgttggt ggtggcgtaa ctggatgtat ttgtgcctac tatctagcta aaaacaatat
    11941 aaagtctgtc attcttgaaa aaggaaggat tgcacatgga agcactagcg tgacaacttc
    12001 tctattacag tatgaattag atgacaattt gatagattta acagaggtta tgactctaaa
    12061 agatgctcta aaagcatata acctttgtac ttcagcactt gaagaattag atacatttat
    12121 agagctatat ggaaataagt gtgattatgc caaaagagat actctacttt atactgccaa
    12181 caaacttgaa gttaaggcta tcaaagaaga atataactta agaaaagaaa atggatttga
    12241 tgttgaatat attgatgaat ccacaaaccc ttttaacttt gatttaaaat caggattgat
    12301 agcaaagaat ggaggacgag aattagaccc atataaatat agtcaccatt tgattgatgt
    12361 aagtttaaaa aatggtcttc aagtatatga aaatacagaa gtaaaaaaag tagatttatc
    12421 aaatgataag gttactgctg aagtatcgta tggatacaaa gttcatggta aaaaattaat
    12481 tgtagctaca gggtacaata caagtttatt tacaaaaaga aattttgcaa ctaagtcaaa
    12541 tacttttaat atagcaacga aaccacttaa aaacatagct tcttggaaaa acaacatttt
    12601 aattagagat aactgtgacc catataatta tctaagaact actaaagaca atcgattaat
    12661 tataggtggt gaagatgtaa gttttgatga tatagaaaat gaaactttag ccaatgaaaa
    12721 gtatgatatt ttagaacaaa gattgaaaag tatgtttaag gatataaaag acattgaagt
    12781 tgaatataaa tattgtggtt gttttgcttc aactttagat aatttaggtt ttattggtcc
    12841 agataataaa aataacaatc tatggtattg cttaggttat ggagcaaatg gtatactctt
    12901 tgcaatatta ggaggtataa tgttaagtga attatatctt ggcaaacaaa ataaaaatat
    12961 gaaattattt aaagtagata gatttgataa atagatatta tatattaaaa gactgtaaaa
    13021 agatgaaact ttataattct aaaagttaac tttgatacag tcttttataa tgttcctttt
    13081 aaaaatactt tatttataat taaatataaa gtaaaatgtt aaaatataac tgataaaatt
    13141 tagtcataag ataatattta agtttatttg atgaaatatg gaggaattat tatgaaggaa
    13201 atactcgtta ttgctcccac taaaggaacc tatgaaaaat caattcacat agttaaaaaa
    13261 aacaaatata caaatattga tgttgtattt ggcaatctaa aagaaggtat acctcttgct
    13321 gaaaaaagta ttaatcatgg tactagaatt atcataagcc gaggtggaac ttacaatatg
    13381 ttaaaagcta cttacaatat acctatagtt gaaattaagg tagatgctta tgatattata
    13441 aaaagttaca aagaggtaaa aaattccaac gaaccatttg gaataattgg atttaataat
    13501 gtcatctacg gtttcgatat tatagaagaa atattgaata agaaaataac tatgattgaa
    13561 attgaaaaag aagaagaaat ttatgatgct atagaaaagt acagaaagaa aggtataaac
    13621 acatatatag gagataccac tgttgcacat attgttaaga gacttaattg taagggaata
    13681 ttaatcgaat ctagagaaga aaacatactt agagccattc aacaagcaga acaaatactc
    13741 gaagcaacca aggatgaaca aaaaagaaga ttacagatag aagctatgac agattttgta
    13801 catgatggaa taataacagt tgataaaaat tttaaaataa cattatttaa taagagtgct
    13861 gaaaaaatat ttggtataaa aaaagaaaat gcactttatc ataatgttgt agatgttatc
    13921 ccaaacacag ttcttccaca agtagttgag actatggaac ctcaaattgg tgaaatacaa
    13981 aatataggga gtacaaaaat tattacaaat agagttccaa tcattgttga tggtcagatt
    14041 caaggggctg tttcaacatt tcaagatagc aaggagataa gttcttttga acatacaata
    14101 agaaggagct tattaagcaa aggtctatct gctaaataca cttttgatga tataattcat
    14161 gctagtgaaa agacaaaaga ttgtatagaa cttgcaaaaa aatatgcttg ttatgatact
    14221 cctatgatta ttactggaga atctggagtt ggtaaagaac tattttgtca aagtatacat
    14281 aactatagta agcgtaaaaa tgcacctttt gttgctgtaa attgtgctgc catacctcca
    14341 tctctaatag agagtgagtt ttttggttat gaggaaggtt catttacagg tgctagaaaa
    14401 aaaggtaaag caggtgtatt cgaactagca catgaaggta caatcttcct agatgaaata
    14461 agtgaaatcc ctcttgaact tcaaggtcga ttattgaggg tattgcagga aaaacaagtt
    14521 atgagaattg gtggtgataa agttattcct atagatgtaa aaattatctg tgcttcaaat
    14581 aaagacttaa aaaatatgat gcgcaatggt ttatttagac aagaccttta ttttagaata
    14641 aatatactaa cattatatct tccttcatta aaagaaagaa aagaagatat tataaaatta
    14701 tcagaatttt ttatacataa atattcaagc aaatacaata aaacgccttt aattatcact
    14761 ccaaatatta aaaaggcact tcttgaacga gaatatgaag gtaatataag ggaattggaa
    14821 ggtatgatgg aaagggctgt tattgtggaa tcatttgact cgattttatt agaaaaggtt
    14881 agcaatgaat atacaaataa tatagttgat aaagataata gtgattttga atgcagtaat
    14941 ttagatttaa aaactttaga aaaaaaatat atctataaaa tttataacga aaataataga
    15001 aatactacaa aaacctgtga aattctaaag ataactcgct ccactctatg gcgtaagtta
    15061 aaagaaatag aagatgatgt ttaaaaataa tacataccta tatatatcaa aacgttttat
    15121 gtttaatttt aatacataag acgtttattt gttttcaata aaaaatacaa ttatatgata
    15181 aaaagaatct ttgtaagtga aattctcgtc aatcgtactt ttaattataa gtaatctaca
    15241 gcatttcaat cgattattaa aaataatcat gataactaaa gatacaaact ctatatattg
    15301 gcatattaat tgcttttata atacctatat aaaaatgaaa gggagtgata ctagatgcac
    15361 atcttaaaat cagtgaaaaa gataccagga ggacttatgg tctgtccctt gttattggga
    15421 gcactattta atacagtatg tccacaagct cttgaaattg gaggattcac aacatcctta
    15481 tttaaaactg gtgcaatgag tatacttgct ctattttgtc tatgcaatgg agctcaaatc
    15541 aatatcagac aagctggtaa accattgact aaaggtatag tacttacagc aacaaaattt
    15601 ataattggtg cagtattagg tattattgtt agtaaattta ttggacctaa aggtataatt
    15661 ggtattacac ctcttgccat agttgctacc atgactaatt caaatggagg tctatttgct
    15721 gctcttgcag gtgaatatgg agactccaca gatgttggag caatatctat cttatctcta
    15781 aatgatggtc ctttttttac aatgcttgct tttggagtaa ctggacttgc aaatgtacct
    15841 ataatagcac tatttgcagc acttatacca atactacttg gatttatact tggtaacctt
    15901 gatgaagata tacgagaatt tcttgcacct ggtacaacat tattaattcc tttcttcgct
    15961 tttcctttag gagcagcatt aaactttaat caaattataa ctgctggact acctggagtt
    16021 gtacttggtt taggagttac tataataaca ggtatgggtg ggtactttgt aatgaaactt
    16081 atacgtgcag aacatccagc agtaggtgca gctacaggta caacagccgg aaatgcagct
    16141 ggtactcctg aagctttagc agctatagac cctactctag ctacaatagc agctgtttca
    16201 acagtacagg ttgctgctgc tattattgtt acagcaatat gttgtccaat gttagtttcc
    16261 tatctagata aaagagaaaa aaagaaaaaa tctattttta aaacaaataa aaatattgaa
    16321 gtaaattaat acttaaggag gtaaaattaa tgaatgcaat tattcatgca aatgaaaaag
    16381 ataatctagt tacatgtgtc agacctttag ttaaaggtga aaaagtttca attgatggaa
    16441 aatcttacat agtaaatgat aatataccag tctttcataa gatggctaca gaagatgtaa
    16501 aaaaaggtga tgttgtatac aaatacggtg aaattatagg aattgcaaca gttgacataa
    16561 aaactggtga ctatgttcat gtacataaca ttgaaagtac acgtggtcgt ggagataaaa
    16621 aataggagga agttaaaatg aaattaatgg gttatttaag agaaaatggt cagtttggta
    16681 ttcgtaatca tgttttaatt attccaacat ctgtatgttc aagtgagaca gctacaagaa
    16741 tagcatcact tgtgcctgga gcaatagcaa tccctcatca acatggatgt tgtcaaattg
    16801 gttctgatat agagcttaca gcaaagacat taataggatt tggaaaaaat cctaatgttg
    16861 ccgcagtttt agtagttggt cttggatgtg atggtattca agcaaaagaa ttagcatctg
    16921 aaatagctac aacaggaaaa aaggtagatt acgttgttat acaggaatgt ggcggaacat
    16981 taaaaacagt ttctaaggga gctgaaatag taagtaaaat ggctcgtgaa gtatcaaaag
    17041 aagtaagagt tgaatttggt atgagtgaaa ttacattagc acttgaatgt ggtggttctg
    17101 acccaacaag tggaatagct tcaaaccctt caataggcgt tgcatcaaac cttcttgtag
    17161 atgaaggtgg aagtagcata ctaagtgaaa ctacagaagt aattggtgca gaacatttgt
    17221 tggctacccg ttttgaagat gaagaaatga aagataagtt tcttaaattt gtaagtgatg
    17281 tagaaaaaag agcaattgct atgggagaag atttacgtag tggtcaacct actccaggca
    17341 ataaaactgg tggactttca acaattgaag aaaagtcact tgggtgtatg tataaggcag
    17401 gcaataagcc attcaaaggt gctcttgaat atgctgatat tgtacctcct gataaaaaag
    17461 gattatattt tatggatact ccaggtcaag atatagattc tatcactggt atggtagcag
    17521 gtggtgcaca aattgtaatc ttttcaactg gtagaggtac tccaacagga agccctattt
    17581 caccagttat taaaatcact gggaatagtg atacatacaa caaaatgcca gacaatatcg
    17641 atataaatgc aggtagaatt ataacagatg gtgctaaaat agcagatata ggtcaagaaa
    17701 tatttaatga aataatagaa gtctgtaatg gtaaacatac caaagctgaa agtcttggtc
    17761 atagagaatt tggcatttac agaatttcat ctacatttta gattgtaaca aaatatcttt
    17821 tataaatata atacttatgt agatacataa gctaaaagtt ctttctaata ctataaaaat
    17881 tttctatact gaaaaggaac tgtatcaaaa ttattttgaa aacagttctt tttttaacct
    17941 cttttactca aaatattata aaataagatt taaacaataa acgtatcttg attaatctct
    18001 aagaagacat aaataaagct aaatgtaaag tgggataaat aaaatttaat atctttgtat
    18061 gaaaagatat ttggtataat atgtctgaga gaattcttat ttgtaaacaa ataaaggatg
    18121 gaggatgaaa caaaagaatg aactttgaaa acacaaaaaa taaaaaaata atagctactt
    18181 tactattgac acttctttta atcgtgtcat taacatgtgt cacttttatt atgaatctaa
    18241 ataaagtaac acataatgaa acagaacgat atcttgtaga agtttcacaa ggtatttcca
    18301 ctacaattaa tgtgaaatgt aaaagtatat ttaggacact atattctatg ggagatactt
    18361 attttgagat taatgatagt gaaaaagaag tcaatagata cttatcaaaa aaattaaaat
    18421 tatttgaatt tgattggtta ggtattatag attctaatgg aaattttgta agtgccctta
    18481 aagatacagg taatgttaaa ggacaagact tttatagaaa agcccttcaa ggcaaagaaa
    18541 caattactcc tgttaaatca caatctggta catatggaat tttatattct gtaccactat
    18601 acaatggcga taaaattgtt ggagctgttg ctgcttggaa tactattgat actatgagaa
    18661 atttattaag tgtagaaagc tttcatggtg aaggattttc ccaaattatc gaccaagatg
    18721 gaaattttat tgtaagttca gcaaacaaaa atgctccaaa aaatgtggta aatttttttg
    18781 acatgattaa acaccaagga aaacaggttg ataaaaataa acttgaagaa atgaaaataa
    18841 acttaaaaaa gaacaaaact ggttctatgt attatacatt aaatgatggt atttctaagg
    18901 caatgcacta tcttccatta caagaagaaa acttatacct cctttcaatt gttccaacaa
    18961 aaacagcaag tgcacagttt gatttacttc taaaaaaagc aatcttcatc aatattttaa
    19021 ttgttatact atttttattg cttattatat tgattatgtt tataaatcaa aaaaacaggg
    19081 cacaacttat aaaaatcgcc tatgtagact ctgtaactaa aggttacagt aaagcacgtt
    19141 ttgatattga agctaaaaaa attatacagt catcttcatc agaaagctat tcattggtat
    19201 ctataaatat tcagaaattc aaacttatta atgattcttt tggtagtgaa gcaggagata
    19261 aaacattaaa gtacatacat gatgtcatta gtaattatct agaaccagga gaattgcttt
    19321 gtcgagtttc taatgaccag tttaatgtac ttattaaaac tattcctcaa aaagaaattc
    19381 ttaagtacat agaaaatatc accaatgata ttaatagttt caataaaaat gaagaatgta
    19441 aatattttat agctctatct ataggtgtat ataaaattga tgacccaaat ctacctttag
    19501 tttcaattca agaccgtgca aatgttgcac gaaaaaataa taaaggaaac attggaaatc
    19561 atccacttta tacatgtgtt ttttatagtg atttagagcg tttaaaaatg attaaagaaa
    19621 aagaaatgga aaatagaatg gaggaagcat taaataataa cgaatttata gtatatcttc
    19681 agcctaaaat aagcctcaaa gataatacta ttgtaggtgc agaagcatta gtaagatggc
    19741 aagacccaaa taaaggcctt ataccaccag acgagtttat tcctttcttt gaaaaaaatg
    19801 gttttattac aaaactcgac cactatgttt ttgaaaaagt atgtagtatg ataagaaaat
    19861 ggatagataa tggagaaact ccaatacctg tttcagttaa tttttcacgt gctaatttaa
    19921 ataatataga ttttttagaa aaatacagaa tgcttcgtaa taaatacaac attcctgcta
    19981 agctcttaga aattgaatta actgaaacac ttgtatttga aaatctccaa aaattaatta
    20041 atataataga ccaaatccat gaagaaggtt ttcaatgttc actagatgac tttggaagtg
    20101 gatactcttc actcaatatg ttaaaagaaa taaaagttga cacattaaaa cttgaccgtg
    20161 ctttttttag ctcacctaat gcagataata ttgctgaaaa tcatgtaata gaatctatag
    20221 ttaatttggc aaaaaaatta aatatgaata gtatatcaga aggtgttgaa acaatcttgc
    20281 aaatggatta tcttaaaaaa attaattgtg atatgataca aggatatgtt ttttcaaaac
    20341 ctctacctca agaaatattt gagaaaatga cttttggaaa aaaactagaa gaaaccagta
    20401 tggatagttc cgtttaaata aaataaaaaa cttgtctcta taaaataatt ttaatcattt
    20461 tataaagaca agtcttttta ttttccaatt taattttatt ttctaaattt cttacactcg
    20521 gcttgtttta aattcccttt ttaagaataa taatacgatt atcatagcaa taaaatctgc
    20581 aactggctgt gccaaccaaa caccatctaa attaaattga attggaagta caatgattaa
    20641 tggtataaat aaaataaatt gtcttaaaag agttaaaaac attgaatgtt taactttact
    20701 tatacactga aaatacacag gtcctacaat agaaatcccc attataggca atgtaattag
    20761 atttaggctt atacctctta ctgctatatc catcaaatct gaatctttat taaatatacc
    20821 tacaaatact tctgggaaaa cttgtactac taaaaatcca aatgacaata tgacaactga
    20881 cactatgata gataatatta aagttttctt tgcacgctca tattgttttg caccataatt
    20941 gtatgatatt atagtttgca taccttgatt caatccaaac acaggcatca taaacatcaa
    21001 taaaatagat gtaagtgctg tcatagctcc aatagctaaa tctccaccat aaacctttag
    21061 agaattattt gtaaacatgt gagttatact tatagctact tccatagcaa aaggtgtcat
    21121 tcctattgca aataccttct ttactatatc tttattcaat tttaagttac atttttttag
    21181 ttttaaattg gacttccctc ttataaaata atgtattacc caaaaaaata ctactatttg
    21241 acagagtact gttgctatag ctgctccttt tatacctaaa ttaaatacaa atataaatat
    21301 agggtccaat atcaggttta atacacatcc tacaatcatt gtccttgctg ctaattttgg
    21361 attaccttct gccctaattg cattattaag agaaaatgca actaaattaa atatacttcc
    21421 aaccaatatg acactcatat agtcttttgc ataaaatatt gaatctttac ttgcacccaa
    21481 aataaaaagt atctcttcta agaaaactaa tcctaatata gttataatta aagagataat
    21541 tatagataat gtcaaagcat tacctaatat cttctctgct tcttctctat ttctttcccc
    21601 taactttata gacaatcttg tgctagcacc tactgatacc aacattccaa aagctattat
    21661 tagagtaaat actggcatag ttacaccaag acctgcaata gctatagagc caataccttc
    21721 catagaacca ataaaagctc tatctactac attataaagt gaagttacca tcattgctat
    21781 tattgcaggt atcgaatacc taaataataa aatccatatt ttttcatgtc ttaatgcttc
    21841 ttgattctcc atgttgtcac catttacacc atgtcataag ttatacaaat tggtttattt
    21901 gttcttgggt ctgttgctat ttctgcatca atattaaata attcttttag attatccttc
    21961 gtcataacat catatgcatc accttcacaa actattttac ctttcttaat acctatcatg
    22021 tggtcagcaa atcttgctgc attattaagt tcatgtatta ccatgacaat agttcttcct
    22081 tgttttttat ttagttcatc taataatttt agtatctcca attgatgtgc caaatctaaa
    22141 tatgttgtag gttcatccag taataatata tcagtttctt gtgccaaagc catagctatc
    22201 catgcccttt gtctttgtcc tcctgataaa tcttccatat ttctatcctt aaattctgca
    22261 attccagtaa cttctaaact ccaatttaca atctctctgt cttctttact agaattacca
    22321 aatccacttt tatgaggaaa tcttccataa gaaattaatt ctgaaacggt aagaccacta
    22381 ggagcttgag ggctttgtgg aagtacagcc atatcttttg ctatttcttt agacttcttt
    22441 ttgtagatgt cctctccatt tatatatatt ttcccactat tagggctaat aattctacct
    22501 atagttttaa gtattgtcga ttttccacaa ccattagccc ctatgatggt agttattttt
    22561 ccttttggta tatttaaatt taaatttttt ataatatctg tgtttccata tgatacattt
    22621 agattcttag tatttataca gttcataaca acaccctttc ctaatcattt accaacatta
    22681 aataaataaa gtacggcact cctattatag aaactactat acctactggt atttctatag
    22741 gtgctagaag atttcttgat atagtatctg ctattaaaag taataatgtt cctatcaatg
    22801 ctgatactgg tattaatctt ttatgctttg gtcctattat ttttctacct atatgtggag
    22861 ctattaaacc taaaaatgaa atactacctg ctactgctgt cgatacacca gataaaatta
    22921 ctgcatatat tataagtctt cttctttgtt tttctacatc tacaccaaga cttatagata
    22981 cttcatcacc caaatttaaa acatctatat atttagattt ataaatagtt atacccataa
    23041 aaatcactat aaatggaagc acagctatta catatttcca acttgttccc caaatgcttc
    23101 ctgatgtcca tgccataacc ctattaaatt cttgtgttgt aaatttcaat tggaatatcg
    23161 tcaaaattga agtaaaagct atgtttacac ctataccaat aagtagtaat cttgacgaat
    23221 ttataccacc tttccatgca agagaatata taagaaatgc tccaaataaa gctcctataa
    23281 gtgcaacaat aggcattgta aatacagtca agttactcat accttcatat acattaccat
    23341 tcataaggta tatataaact actacaaaca atgcagcacc tgaatttatt cccaaaattc
    23401 cagaatcagc taaatcattc tttgtaactc cttgaagtat tacacctgct gttgataatg
    23461 cagaaccaac caaaagtgct attactattc ttggtaatct taatttaaat attgcaattt
    23521 catgattacg ctgaccctgc cctaaaaaag tctttataac atccattggt tctatagaaa
    23581 atgagcccat atttaaactc aataaaaaag tgataaaaat aagcaccatt aaaattgata
    23641 taacaagtaa tgttttagaa tttattttta taatattttt catagtacct atttctcccc
    23701 ctttctaaca aggtatataa atattggcac tcctataagt gctgttatag aacctactgg
    23761 tgtctcatat ggaggattta tcatcctact taatatatct gtatatacaa gcaatacagc
    23821 tcctaatact aaagagctcg gtattatata cttataatct gaacctacta ttcctttagc
    23881 tatttgagga actataagac ctataaaaat aatattacca gacactgaca cagatgctcc
    23941 agtcattaat attacaagta ctatacatat aaatctaaca aacttagttt tttgtcctaa
    24001 tcctattgcc acttcttctc caagacttaa tatagttatt cttggagcca taataatagc
    24061 taaaataaat ccaataccac cagctacaaa tagcaatttt ataccttccc acttcactcc
    24121 tacaagccct cctgatatcc agaaacttaa ttgttgagat aggttaaaat acatagatat
    24181 tgctgatgcc aaagaaatta gcaatgtacc aagagcgact cctgcaagag ctaattttac
    24241 attattaact cttcctcttg atttagaact tataaaatat ataaataatc cacttatact
    24301 tgctccaata aatgcaaaca tcattacact aaaacttcca tgtataagtt gaggaagttt
    24361 tttttgaagt accagtgata tcgaaattgc gaatgtagct ccttgtgtaa ttcctataac
    24421 agatggttca gctataggat ttctcgttat tccttgcatt attgccccag aaactgctaa
    24481 aaaccctcca acaagtgcag ctgctattgc ccttggaagt ctaacatcct ttactattct
    24541 catatttatt ccatcttcca tttttatcaa actatctata acagttgata gattcatatc
    24601 ttttgcccca atagttatgg ataatacaat tccaaataca agtacaacta tacttactaa
    24661 tatcaacaaa taagttatat tttttttact cagcttagtc aattatccta cctccttttc
    24721 ccttttaatc gtcagtataa tatcatctat taagtatttt aattttaaga tttattaaaa
    24781 ataggtgtga aaaatgaata tcattatctc acacctacta agttaagaat tactttgtta
    24841 attcattttt aactgattct aataataact cttttccaat tggattgtat gattgactga
    24901 aaaatggagc agcatcaagt attgttacat taccatcttt tactgctctt atttgagccc
    24961 agatagcact attttctaaa tcttttttat ctgcttctgt agcaattaca actatatggt
    25021 ctgcatctat atctgttaaa ccttccatac ttaccattgg tagtgttatt ccatcctgtt
    25081 ttggcatatt cttaggtcta gctaatttca tatcatcatt gattagagta cctattccac
    25141 catcactaaa taccataaat tgacctgaac ttgccaacac tggtaaataa gttttttctc
    25201 cattcttttc tataacttcc ttacctaact ttgtagcttt ttcatcatac ttctgtagcc
    25261 aacttttatc ttcttcttct ttgtcaaata actttgaaac atcagttaac ttgcttctcc
    25321 agtcatttgc ataatctttc atcataacta caggagctat ttcttttaat tggtcatata
    25381 ttttttcttg tctttgactc attattatta aatcaggatt tacttctaat atagcttcca
    25441 tatccatagt atccatcatt gagtgcccaa ctatctttac acctttaagc tcttctctta
    25501 tgtaacttgg taatttatca gtttcatatg agtctacatt tgctgttgca acaggcttat
    25561 aacctgctaa taatagttct tcactacttc ctgatatatc aactattttt ttaggatttg
    25621 atggtatctt aacctctcct ttaacagatt gtacaactct tgtctctgat ttatctttat
    25681 cctctgtatt cttatctgaa catgctgtaa caagtactaa cgtagttata atagatataa
    25741 atattgcaag tgattttatt tttttcatga ttacttctcc tacttgttta ttatacttac
    25801 atgatattga ttatcataag cacatattta ttatagcatt tttctatttt tagtcaatat
    25861 tattttgtaa aaggataaaa attcctaatt aatcttaatt atatatagct tagataaggt
    25921 tttcttatat taaacagtat tttttttaag aattggacac aaatacaatg acttttcagt
    25981 taaacaatga taaaatgcaa tttatagata aggaatttag tagggaggat tcaaaacatg
    26041 gtacatttag tatattgtga taatgcagga aaaaaaggag aaaaggtctt agataaaatc
    26101 ttggatggaa caaaaacaat ggttattaga ggtgctgctg gtagaaaaat accacatagt
    26161 agagtatttg aagatgagtt actttatttt atggaaaaag gaactgcaaa aatatctgca
    26221 aaagccactg ttaaaaaggt agaaaactat gttaaattaa cagaagatga aatttctaaa
    26281 attttaaatg aaaatcaacc aaaattaaat ttatctgaaa agcaaaaagt tcgttggcat
    26341 aaaaaatgtt tatgcttagt tgaatttgaa aatgttgaag aaataacacc tcttgatttt
    26401 gaccatcaag gaaatatgga tgattggctt attgttgaaa aaattgaaga tgtagttgtt
    26461 ggtactagta taccttacaa ttatgagaag tctaaattct aaagtatcaa taacataata
    26521 ctatattgct aaactctact taaataaaaa aagagtgcat ttaataatta taaaaattat
    26581 ttttatacac tcttttttaa taataatttt aaattactta ctttctaatc tttcatatac
    26641 atctataaat tcttctgcta attgttgaaa attcatagct gtcattaaat ggtcttgtgc
    26701 atgaataagt ataacactta tttcagtttt attaccatta gcttcatttt gtatcaattc
    26761 tgtttggaat ttatgagctt tatgaattga ttctttcgac ttttcaatac attctctagc
    26821 tttatctata ttcccgctct ttgcttcttt tattgcttca aatgctagcc ctttcgcatc
    26881 acctgcaaat ccaattattc caaaagatat ctctatcatc ttctcatcca tttattcgac
    26941 ctcctaaata tctctatagc ttgatatgac taatccattc ttttctaaaa attctttgac
    27001 ttttttagat gtaagtatct tgtgctcttt cgttctatca atagcataag atgtagaatt
    27061 taaaatataa tcatctaaaa aagctgggtg agacataata tccactacat catacttcat
    27121 tatttcgtct atatttttta taaaatattc ttcacttaca ttttccttat aaaaactatc
    27181 tattaaagga actactttag aatactctaa attatattca aatccacctc ttattggaag
    27241 attatacttg gttacaatct tctctattac tggctttaaa cttactaaag tatggatatg
    27301 atggtgacta tctaaatgag atatatctat tccaagccct tttaccctat ctatttgagc
    27361 acaaagttct ctataaatct catcacaatc cattttttca ataatttcgt ctgtaatcct
    27421 cctaataaaa aaaccgtctt tatcaacaat agtttttaag ttagataata atggtctacc
    27481 acaacttaaa gtcatatgaa ctccacaatt taaagtttta ttttctttaa gtaagcctac
    27541 tccatgttct accccaggca tatttgccat catagaagta gacctaacta taccattatt
    27601 atgagctgaa attattccat agttcacagc ttcacaatat ccaaaatcat ctgcatttat
    27661 tataatttta gtcataccat aacctacttt ctatagacaa ctatctatta aatctaggaa
    27721 gataatcttt atgagcttcc aacatttcgt ctaatatagt ttttgctaag tcatcagatg
    27781 gaatcaatgg gtttatacaa agtgccaaaa gagcagattc ataatcgcca tatacagctg
    27841 ctttagctgc caatacttca aatgatttga tttgacttac taatccatgc acagaatctg
    27901 gtaaatatcc tatacttatt ggttttggac cattttttgt tattacacta cttacttcta
    27961 ctgcttgttc atctttaaaa tctcttatag aacccttatt taatgtatta actacttgga
    28021 tatctttttt gtcattgtat atagaactta ttaaattaca agcagcatca ctatagtaag
    28081 cccctcctct tttttcaagt tgaggtggtt ttacatctaa tttttcatcc ttatataatt
    28141 caaataattg ttcttccaat tctttaacta cttgacctct agcttttccc tctttaaact
    28201 ctctcaactc atcctcaagc atttctttag ttttatagta gtatctatgg tatggacaag
    28261 gtattgcacc caaagatttt acaaattcag cattaaaatc aattgccttt atattttgca
    28321 tacttatatc tgctttgaca aatttatcaa ttgcttcttt tgtaacatct tctccatcta
    28381 aagatacatt tagaccataa accatatgat taagacctgc aaaatccata cttattctgt
    28441 cactttctac attaaatagt ttggcaacat cattttttaa atgaattggc acgttgcata
    28501 gacctatata tcttctaaaa ttagtatatt tgaatacagc ctctgttatt attcctgttg
    28561 gatttgtaaa attcaccatc catgcatttg gacataattc ttccacatct tttattatat
    28621 caaatataac tggtattgtg cgaagggctt taaataaacc tccagcacca ttagtttcct
    28681 gccccataac tccatgagat aaaggaatag tttcatcttt tattctcgca tctaaaagac
    28741 ctactctaag ttgtgttgtc acaaagtctg catctcttag agcttctctt ctatctaaag
    28801 taagatttat tttcatatct actccagctt tttttaccat cctcttagct agatttccaa
    28861 ctatctctaa tttttctttt ccagcttcta tatctacaag ccataattcc tttacaggta
    28921 actcttcata tctatttata aaaccctcta caagctcagg agtataactt gaacctcctc
    28981 caattgtaac tatttttagt ccctttttca tctcattata ccatccttac ctataattta
    29041 atatactcta caaaacccca tacatttatt taaaactcta tacatttatt taccgaaata
    29101 aaactaccta gttgttaatt ttcaatttat tagcttccat tttttcagac gctatgacaa
    29161 atggaatata tattaatatt gataatatta aattaaatag tgctaaagct ccgccagtcc
    29221 agtcacctgt agccattgct ccacctaata taggaggtgt tacccaaggt attttaggta
    29281 aaattactgg tgctactaat ccaaaatcta ttgctaagta tgatacagca ctacatatta
    29341 ctggtgcaag tacaaatgga atcataaata taggatttaa aactattgga agtccaaata
    29401 tcaatggttc tcctatgtta aatattgcag gtggaccacc taatactatc atctctttac
    29461 gtcttcttcc agctattaat aaagctacta ttaatcccaa taccattcca gaaccaccta
    29521 aatatacaaa tgcatcaaag aatgcacctg caagtacatg gtatccttcc gttgctcctg
    29581 cttgagccaa ggcagcattt tctccaccta atttcataag tatagtttct gtaaaaggaa
    29641 gtgctatatt tgcaccatga agtccaacag tccaaagtat atgaattata aatgcaataa
    29701 gcattgtaga acctagacta tcagctacat ttgatagtgg tgctcctaaa taagtattta
    29761 aaatatctgt taagaaactt ccattagaaa gcatttttat aaatattcct attacagtaa
    29821 atatcattat agttaacatt cctggtatta actttgcaaa tgatcttgaa actgctggtg
    29881 gaactccatc tggcattttt atagttaatt tctttacctt agataatctt acaaatatct
    29941 cagttgataa taatcctatt aatatagaag agaataaagc tgctgtacct aagtaggttt
    30001 gttgtatcat tccccatcct tcaactactg tagttcctcc ctcaggaact attttaccaa
    30061 tttgtggagc cattataaaa aagatagata aagctataag cccagattgt aacccatcac
    30121 tctcataaga ttttgctaga aaataagcaa cacctattac taagaatact gccattgtac
    30181 ctattgctcc ccaccaaagg tcgcctccaa atgtggtcca gttttctcca aatatacttg
    30241 ccatcaaatt tttgtatgct tccaatggta agttatttat caatgttccc aatgcaccta
    30301 ccatagttat aggtatcatt actataaatg catctcttac tgctactaag tgcctttgag
    30361 ctcctatttt agcagctaca ggcactatat atttatccat aaatgacatg aatttttcca
    30421 ttttacatcc tccagtacaa ctttattttg aactatttaa ctctacagct ctatctaaca
    30481 cagcttttcc attacatctt ccataatcca tcatatttat aacttctact ggaatattat
    30541 atggttttgc catttcagtt gcttttccta atacatatct aacttgaggc cctaaaagta
    30601 agacatcaca attttctatt tcatttttta agttcacttc tgatatagcc catatttttg
    30661 cttctatacc gttttccttt gctgcatcct ccatttttgt aactaataaa ctagttgaca
    30721 ttcctgctga acaagctaac attattttta tcattttttc ccctccaaat tagattcatt
    30781 aaatattttt taagtcataa gttttttaaa ctatacattt aaaatatgtt tttctattaa
    30841 ttttttctta atgtatatat cttattttta aatgtatata catttaattc ttgatattat
    30901 tatataccaa atgtatatac atttcaagaa aactttttca ttttatttaa attttattcg
    30961 acaacagcat tcaaagttaa tgtatatcca tagtgatatg tgtgtgagta ctcaaaaatc
    31021 tcactatttt gtaacttaca aatttggctt atatataaaa gtggctctgg ttcttctaat
    31081 tttagattat ccatacactc ttcattcgat attacagctt gtacttctct ctctgacatg
    31141 gctatcttgt attcacacac ttcttcaaca aaattataaa gagaattttc acaatgagtc
    31201 ttatttaaat ttggaaataa cttaactggc atataagtaa tcatatatga aactcttgca
    31261 tcattggcta atcttactct ttttatttcc caaacatttt cttctaaact tatattcaac
    31321 ttacttgata aattttcatc tgcctgaact atttcaagag ataccaatat attagatact
    31381 ttatagcctt tactttccat ctctttagta aatccactta cagacgatat attatcatat
    31441 atattatttg caataactat actacctaca cctctttgtc tagttatata gccttcttta
    31501 actagattat taagtgcaga ccttacagtc attctagtta cattaaattc ttcactcagc
    31561 tgtttttcag aagggattaa atccccttct ttcaaactgt ctgaatttat taactctcta
    31621 acatgatttt ctataacttt ataaattggc tctttcattt aattcactcc ttattaaatc
    31681 ttttatataa taagataatt ttatatacaa acaaagaaaa tgtctataca tttaagataa
    31741 tttaaatttt attcagaaat cttgtacaac tttccgagtt ttaataaaga tataaataga
    31801 cttatcatta atataccttt taatatactt gataaggtta tactccacca tacaccattt
    31861 agacccaaca tttctggtct agatataaag taagccaatg gtactcttgc accagtcagt
    31921 atagtaatta taatagaagg tatataggtt ctccctaatc ctttgaaagc tcctgttgtt
    31981 attatttcta aacacataaa aaattgagaa tagcctaata ttttaagata aactgctcct
    32041 ttttctattg cctcactttc atctataaaa atagaaaata tactctctcc tgcaaacact
    32101 aatactaaag tagttattat accaagtaca gaagatacag ttatagctat tttgcaacct
    32161 ttatttattc tagaatactt cttggcacca taattttgtc caacaaaact gcctaatgca
    32221 actgatagcc cttcagccgt catccaggaa attgcttcta tttgtgagcc tactttttgt
    32281 actgcaacag ccacaggccc ccatgatgct actattactc ctaatagcat tgagaatact
    32341 gtaaacatac cactttgtat tgctataggt aatcccaatt tatacagaac tttataatat
    32401 ttagtctcta tttctttaaa tagtcttaat ttaaaatatt cttctttact tttaactatt
    32461 atatatataa aacatgtagt aactactatc tgagcaaata cagttgcaat agcagctcct
    32521 gcaacaccaa gttttggagc aaatccccat ccaaaaatta atatagggtc aagtataata
    32581 ttagttatta atcctactgt attaatacga aaaggagttt tactatttcc aagaccattg
    32641 aaaattgcag taaatactgg attaataaaa taaaatacca tacctagagc tactattatc
    32701 aaatactgtc tagacatggt tataacttct aagtctccta atctaaaaaa acctatcaat
    32761 tgtttattaa gtactattaa agaaattgta tatattattg ctagtataat atttatttct
    32821 attgctgact tcatatatga tttagtagtg cttatattat ttgcaccaat actttgtgct
    32881 acttttatct ctccacctac ttttgaaatc ataataaatg ccattgcaag ccatggaaaa
    32941 aaaccagctg ttccaactgc agctactgct ttacttccgg ctttaccaac ccatatcata
    33001 tctatcatgt tataccccat ctgtataaat gaagtcccca ttattggaag tgatagctta
    33061 agtaattttt ctgtaattct tccttctgtt aaattaagtc tgtctttcat tttccacctc
    33121 ttttatatta aatttatttg cttatatagc tagattaatc attttatttg attgagttca
    33181 ctatccttca atttatatta tcatatctca ggattgcatc tcaatcatac ttatgtttaa
    33241 gatacagtat tttacctatc tatgataaaa ttaatactta tgaatataaa tctagccatt
    33301 tcatcaatac atattctaac ccttaactta acttcaatta agtatacata tcgtaaccaa
    33361 ttagaatatt ttatacggta tcttccatta ataaacaacc aatatcaatg atttatatat
    33421 aattttattt atacataaaa tagtatacta taataataaa aaaactatat ttgaatgctt
    33481 taagcatgtt tactaattgt aatttccaca caaatttgat aatatatgaa tatattatgg
    33541 tgaaatttat agatatatat taattatggg gagattgtgg gaggttcttt tcacaagaca
    33601 tttaagctat gaaaataaat aaaaaattca atagagttga tatgcaggtt tcaatactta
    33661 ctgcaattat tgtaataata tcttcctcat gtgtatattt ttttaattat acactaactt
    33721 ataagagcat gatatactct ttaagtgaga ggtcaaaaca tatctatgaa tatgtagaaa
    33781 gaaatattga taaaaatacc tttactgaaa ttaattctaa agaagaccaa tcaaaaaagt
    33841 cttataaaag ttctaaaatc cttttagaat ctgtaaaaaa tactactgga gtcatgtatc
    33901 tatatactgc taaaaaaaca aaagatggat cactagtata tgttgttgat ggtttaaatt
    33961 catctaggag tgattttaga aatgctggtg atttgataga aaaagagatt tggggagacc
    34021 ttaacaaagc attagataat catatattgt tacctaaaaa gattaaatca acatcttggg
    34081 gatacatatt tatatcgtat tttcctattc acaatgatgt tggtaaggtt gttggagtaa
    34141 taggaattga atttgaagca aaacatcaat acaatacatt taaatttata agtatagtta
    34201 caccaataat agcattatta acttgtctaa tatcagcatt gattgcagtg attctattta
    34261 aaagaatatc taatcctaca tatactgatt ttgctaatac agattattta acaggcctaa
    34321 aaaatagaaa tgcttttgaa atagatatga ataacttaaa caattcaaat ctaaagaatc
    34381 taatatctat catatcaata gatttagacg gtttaaaaaa gattaatgat aaatttggcc
    34441 atcagacagg agacttatat attaagcaag caagtaaaat tatacaatat gtaataaata
    34501 aaaaatatat agtttatcgt attggaggtg atgaatacat aattattcta aaaaatttat
    34561 ctcacaaaga aataaataat attataaata atattaattt aaaaatagtt gaagaaaact
    34621 caactaatga attaaaatta tcaatgtcta ttggatatgc tatttttgat gaaaaagtag
    34681 atgtttcttt atttgataca tatcgtcgtg cagattctca aatgtatgat aacaaaagga
    34741 aaattaataa acaatatcaa taataaattt aaaaagtcta ttctttaaat aataaatcac
    34801 aagaatagac ttttttaaat ttgtgttttt ataagataaa ttatttaatt aaagtttgtt
    34861 cttttttcta aacgattctt tataaattca tatattactt tcactaatat atttgtgact
    34921 aatattaaca cagctagtgc tgctgcctgt gcagtactcc ccttatcatc tagttgtaca
    34981 attgatattg cagctacttt tgaacttgca gtatatataa aaactaatgc tgatattgtc
    35041 accatggagt ttaggaaaaa gtacataaag ttctctaaaa ctgctggcaa acaaagtggt
    35101 atagttacgt tcttaagaat tgatgcgctg tttattccca ttgatttagc taccatatct
    35161 aattctttat ctactttctt aatagcagta gtagctgtta taaatgctac tgaaaagaaa
    35221 tgaactatat tacatataac cattataagt aatgttccat ataatccatt aaacaaattc
    35281 ttaatgtata ttgtatccat aaaattaaac tctaatttat taaaaaataa tacataagct
    35341 atacctaaaa ctaatcctgg caatgccata ggcaatgtac ttaaaaaata cccaaatgat
    35401 tttaaatatg gagctttttc tgttttttca ttcaaatatg caaataagaa aacaaagctc
    35461 gtccctagaa tagctgtaaa tagtgacatt tttattgaat ttataaatgg tttaatccca
    35521 tcaatactgt ttttcataga aaaatgttcc agtgtcagtc ttaaatcata tggccaaact
    35581 gatattaatg aagataagat tacagttaac attacagcta ttataccaaa tgcaataatt
    35641 cctacaaccc ctcctaaaat actatctctt aacttattag ttttaacctt atatggaatt
    35701 gactttgaag ttatatatga attttgattt ttttcaattt ttttagttac tataaacgat
    35761 attattgcag gtattaaaag taatattgat acagttgctc ccattggcat attctgttgc
    35821 cctataattt gcttatatac atctattgat aatacattaa actctcctcc tatcaccttt
    35881 ggagctccaa aatctgtaaa acataatgta aacgatacga atgcagaact tattaaagag
    35941 tattttatac ttgggattgt tatgcttata aattctctta tcttgcttat ccccatagta
    36001 ttactagctt catatagtct ataatcggtt gaacttagag atatataaag tatttgaaat
    36061 gattgaggaa atacatatat aatttgtgcc aatattattc ccactgctcc atatattgga
    36121 acatgcactc caagtaaatt agttaccaaa ccttgatttc caaataaata tactagacct
    36181 attgcatgca tcattgttgg agcaaacata ggaaacatag ctaatgattt aaaaaaacct
    36241 ttaaatttaa tctttgttct agttaatgca tatgcatata ctaatgcaag tgttagtgat
    36301 attacagttg atataactga tacaaatact gtatttttta gagaatttaa tagagattca
    36361 gaagccaagt attcttgaaa gttttgtaat cctacaaaga ctccatcttt atctaaaaaa
    36421 gcctgtacaa atagatatat tataggtgct aataaaaata ttactaaaga cataactaat
    36481 actgtaagca tacatttttt tatatctaaa ttcatttttg atttaagact tatactttta
    36541 gaacttatag cattattatt catacactta tgctccttta acttttggat actttaaata
    36601 gtatttttcg tctatactta tttttacaag gtcaccttct actaaattta aattaaccca
    36661 ctcttttata gaaacatcac ttattatgaa attttcattt aatttatctt ttatttcaac
    36721 tctcaataaa ttccctctaa actctatatt tgatattatc ccttggtagt tttcttttgt
    36781 cgatttctct atttgaacat attctggtct tacagttaat atagaatctc cattatcaaa
    36841 acagtttgta tctcctataa attgagctgt aaataggttt tgaggatttt ggtatatttc
    36901 ttctggagtc cctatttgca ttatatctcc tccatttaga atagctattt tatctgccat
    36961 agttatagct tcttcttggt catgtgttac cattattgtc gttatattaa gttctttttg
    37021 taatctttta atgtccattc taagcttgtg tctaaccttt gcatctaatg cagacattgg
    37081 ttcatctaaa agtaaaaact ttggttcaag agctaatgct cttgctattg caatcctctg
    37141 ttgttgtcca cctgataatg ctttagggta tttatgagct tcattagtca atccaactgt
    37201 ttcaagtact tccttaactt tattatctat tttttctttt gaaacttttc tctcttttag
    37261 tggaaaagct atattgttat aagcagtcat attcggaaat aatgcatatg attgaaatac
    37321 tataccaaaa cctctctttg atggctctaa atttgtaata tctttatcct ggagaattat
    37381 agttccacta tttacatctt caagacctgc tattattcta agtaatgtag ttttaccaca
    37441 tccactaggt ccgagtaaac atagaaattc cccttcttca atatccaaac taatattatt
    37501 taaaaccttt ttttggtcat aacttttaaa cacattattt atttttaagt aactcatatc
    37561 ttatctctcc ttatatagtt atttttttat tttaaatttt atatcctact ttttcttcta
    37621 aaattctttc atctaaatta ttaatttaaa ctttacttac tttcagattt tgaaccgtat
    37681 tttgattccc aagttttaag tactgtactt ctgtttttgg cagcagagtt taaatcaact
    37741 tcttttataa gctgttcaaa tggttgttta gaataacctt ttggaactgg attatctgtc
    37801 tttatggaag taactgctac ttcatgtgca tatctatcca tagctgaatc acttattgcc
    37861 caatctaaaa atagctttgc ttcttttttt atattatctt ttttaactaa ggcatttgct
    37921 tctaagtccc aacctgaacc ttcctttgga aatacaacct ctataggttc tccactagat
    37981 aattgttgtg tagctctata tccaagtgta atacctattg gatactctcc agagccagct
    38041 aacttcgcag gttttgaacc cgagtgtgta tatgtggcta tatttttatg taatttatcc
    38101 atgtaattgt aagcatcttt ttcacccatc atttgaataa gccctgatac tgtaagatac
    38161 cccgttccag atgatgctgg atttggcatt gttataagtc ctttatattc tggttttatt
    38221 aaatcttcat aactttttgg aatatctata cctagtttct taagttcctt ataattaact
    38281 actattgctg tctctgggac actcattcct acccaagata catcatcttc agtatcttta
    38341 aattttttat caactttatc ataattttta ggcttatatg cttcaagtgc accatctttt
    38401 tttagctcta tcatgttcat tgctgataat ccccatataa tatctgcttg tggattatct
    38461 ttttctgcta atagttttga agctattaca ccatgagaat ccataactat ctctacatct
    38521 atattgggat actcttctct aaaaccttcc atataataag ctatctgctc ttcctctagt
    38581 gctgcatata ttgtaagttt attatttcct tttccttgag tagtttgtgg tgttgttgat
    38641 atgcaacttg tcataaatgt aatcactaca atgcacagac aagtaaatat aaattctttc
    38701 atatcttccc cctttgtgtt ttttcctttt tttatttgtt ttttattgtt tttgcttatg
    38761 tttatataat acacctaatt tgttaatata aagttaagtg taaattaaca gttcgttaat
    38821 ttttagaaat ttttgttaaa tatattattt ttagttaaat acagtctgtt tttaaacaat
    38881 atattaattt ttatagtatc tacattttaa tttaaacata aaaaataagt ctaacctcta
    38941 taaagtagaa tgttagactt atttttataa gtatttaatt atacaaccaa atcatttttt
    39001 ttcttttctt caagtgcttt tttaagcacc atatccttaa ccttcataag tctagtagta
    39061 cttgctctct cattttcttc aagcttcata gctatatact taatagtctc aatgtaattt
    39121 ggtatcatta cattttcaag agcattaaca cgtctacgtg tcttttctat ctcttgtgca
    39181 agaagctgtg cacttttttc actttctgca agtcttaaaa gtggttgcat agcatctgag
    39241 aaagcttcca tagcagaatc taattcacct gatgtaaaag caagaccata aggataaata
    39301 tcactttgat tgttttcagt tttaaagtca aacacaggaa catcaacact cattatattt
    39361 ctcgtagaaa catctactga aactgattgc tttggcatca taagtgctga acccagatac
    39421 tcctgactca taactgctct tgcgattata aagtttttat aagcagtatc aagagcattt
    39481 tctgcttctt cacgcaatcg cttattttct cttacaatct caagaaattg tttcataagt
    39541 tcatcaagtt tatctttaag aagcttatga cctcttgtag cagttttaag gagtttctta
    39601 agtctagtca tttccatacg tgttggattt atatttaatc tagccacaga tatcactcct
    39661 taggcatata tttttcaaaa tattcatcac ggatacgttt aagttcactt tttggtaaca
    39721 tactaagaag cttccatccg atttctaatg tctgctcaat agttctatca gttctaaatc
    39781 cttgtgaaac atattctttc tcaaattcat ctgcaaactt agcatacatt aaatcaatct
    39841 cagaaagtgc agattcacca agtatagcca taagttcttt tgcatcctta cctcttgaat
    39901 aagctgcaaa aagctggttc attgtatctg catggtcttc acgagtctta cctttaccaa
    39961 tacctttatc ttttagacga gaaagtgatg gtaaaacatc tataggaggc tgtatatctt
    40021 ttttataaag ttcacggcta agtataattt gcccttctgt aatgtatcct gtaaggtcag
    40081 gaattggatg tgtcttatca tcctcaggca ttgtaagtat tggaatcata gttatagacc
    40141 cttcatgccc tttcattttt cctgctcttt catacatagt agcaaggtca gtataaagat
    40201 atcctggata tccacgacgt cctggaactt cttttttagc tgctgaaact tcacgcaatg
    40261 cctctgcata gtttgtaata tccgttatta taacaagtac atgcatatct tgctcgaaag
    40321 caaggtattc tgctgctgta agtgccattc taggtgtaga tacacgctct acagctgggt
    40381 catttgcaag gtttacaaac actacagaac ggtcgattgc accagttgct ttaaaatcgc
    40441 ttataaaaaa ttcagcatct tcaaaagtta taccaactgc agcaaataca acggcgaact
    40501 tagaatcagt tccacgtact tttgcctgtc ttgcaatctg aactgcaaga ttggcatgtg
    40561 gcaaaccaga ccctgagaat ataggaagtt tttgccctct aaccaatgta ttaagtccat
    40621 ctatagcaga gactccagtt tgtataaact cggccggata atcacgtgct gctggattta
    40681 ttggagctcc atttatatca agtcttttat ctggtataat ttctggacct ccatcaatag
    40741 gtctacccat tccactaaaa acacgaccta atatatctgg tgaaactcca aagtcaagac
    40801 tctttcctaa aaaacgaact ttactttcag caaggtttat acctgttgag ctttcgaaaa
    40861 gctgaacaag tgcagtatct ccatttacct ctagaacttt gcaacgacga atttctccat
    40921 ttgaaagttc aatttctcca agttcatcaa attttacacc ttctacgccg tttattaaca
    40981 taagaggacc ggctacctct tgtattgact tatattcctt tatcatagtt cttcagcccc
    41041 cctcttttta ataaggtctg caagctcgtt gtctatttca tcttcaattg aatcgtaagc
    41101 tgcatctata tcgccttctt ctacatactt agcacgacca atcttttcaa gaactgaaag
    41161 cttgataata tcgtttatag taacattttg cttgatagca tcttgtgctg acttatagaa
    41221 ttttaatata agtcccatca ttctatattg tttatgaagt gatgtataag tatcaacttc
    41281 atggaaagag ttttgatgaa ggaagtcttc tcttatagaa cgagtaacct ctagtatcaa
    41341 tctatctcca tctgaaagtg aatcaacccc aaccaattga actatttctt gaagttcaga
    41401 ttcaacttgt aaaagtttca tagcttgagc tctttgagct gagaaattat catttacatt
    41461 tttatctgcc catacatcaa ctttttcctg atagagtgaa taactagtca accagttaat
    41521 tgctgggaaa tgacgtttat atgctaaaga tgcatcaaga ccccagaata cttttactat
    41581 acgaagcgtt gcttggctga caggttctga tgtatcacca ccaggaggag aaactgcacc
    41641 aattgctgta agtgttcctt ctctttcatc catacctaga caatttacct ttcctgctct
    41701 ctcatagaat tgagcaagac gtgaacctaa gtatgcagga taaccttctt caccaggcat
    41761 ctcctctaaa cgaccactca tctctctaag agcctcagcc catcttgatg tagagtctgc
    41821 cataagtgca acactatatc ccatatctct aaaatattca gctattgtaa taccagtgta
    41881 tatagaagct tcacgtgcag caaccggcat atctgatgtg tttgctataa gcacagttct
    41941 ttgcataagt gactcgcctg ttcttgggtc ttttaattca ggaaattcaa gaagaacatc
    42001 tgtcatttca tttccacgct cgccacagcc tatatataca actatatctg catctgccca
    42061 tttagcaagc tgatgctgtg taacagtttt tccacttcca aaaggtcccg gtatagctgc
    42121 taccccacct tttgtgattg ggaaaaatgt atctataact ctttgacctg taacaagaag
    42181 tgaatctggt gttttctttt ctctatatgg tctttctttt ctaactggcc atttttgcat
    42241 catagttaca ggtatatcat tacctttttc atctgtaata acacaaactg tatcttcaac
    42301 agtaaattca ccagaatata tttcttttat agtaccctta actccataag gaaccataat
    42361 tttacattct actactgctg tttcttgaac gccaccaatt atatctcctg ctacaacctt
    42421 atcaccaaca gattttttag gtttaaattc ccacttagtt gttctatcaa gtgaagatac
    42481 ttcaacacct ttagttatat tagtacctga aatatcatac attttttcaa gtggacgttg
    42541 aataccatca taaatagttg aaataagacc tggtccaagt tcaacactca taggcattcc
    42601 aagagaaact acttcttccc ctggtcctag accggaagtt tcctcataaa cctgtataga
    42661 agctttatct ccatgcattt ctataatctc gcctataaga tgtttatctg aaactcttac
    42721 gacgtcaaac atattcgcat ctctcatgcc ctccgctaca acaagcggtc ctgacacctt
    42781 tacaattgtg cctgttttca tattctctca cctgcaatca tatactttat caaaatttaa
    42841 atattccctc ctatatttta ttagcacata tatttagcac caataaaatt tgagattgtc
    42901 ttttattcaa ctttcatcta ttctttaaat aatatatctg cacctatagc acgtttacta
    42961 gattctctta cttcattcat acctaatccc atacttcctc caatacctgg aataactata
    43021 atggcaggaa gttggtaatc tttgtatttt gctattgatt cttttgcaag caatgatgcc
    43081 tgttctgtaa tataaattat cgcatattca tcttcaacca acttatgtat acttttttta
    43141 atttcgtcac tatcataggc tgggaagata tctattccaa gagctaaaaa tcccatgatg
    43201 ctatctttat ctccaactac tcctacctta tacataagca tcccttagcc tttcttttat
    43261 agtatctgcg tctatattat taagcttact agtcaaaata attctaattg tttttatctc
    43321 tgattcttta gcatataaat atgcaattag tggttctaat gtaagaggct taaattttgc
    43381 tcctctaaca tattccataa gatagtcatc acaaagtttt tcaaagacag taaaaccact
    43441 atccatgcca tttttacaga catcagagta agaagtagat ttaaaacatg atgctggagt
    43501 atctgaggaa aaagcttcta agaatttttc cttatcaagt gagccacctg aaacaaacac
    43561 attcataaac atatcaaaat ctttcttcat atttttgact cttataaaac tctttagatt
    43621 tgttaaatca caaactttca taacataatc tattacaaat ttgttttggc tttctttagc
    43681 agtttctttc atactggtaa aggttgcttt atctagaaca atatcaacca tctggccatt
    43741 ttgtgtctta ttataaacat caaatgcctc taatatagct gatgacatta tcttaggtaa
    43801 atcactaaaa ctacgatttg ctaaagattc tctaagctta ataagaggaa ttgtacctcc
    43861 atcaattaaa tatttctcac catctacacc agaaatttct tctttaatca gcacttttaa
    43921 attgtgatag tcatttttat aaagaaatac atcaacaaat ttttcttcag gagctagact
    43981 tttaagaatt tcatatgtct ttgataattc ttgagtcaaa atattctcaa aattatgtat
    44041 attattattt gatgtatcag cataacctgc ctcagctata attcttaaag agtcttcagg
    44101 gcttttggct tctgccattt gaataaacat ttgtctgtta agaagctgtt tttcaagcac
    44161 tcttattcgt gcaacggcat agggataagt tttttcttct gccatttttt aacccctcct
    44221 taattaaaca aaatttctgc tgcaatttgt tcaatatatt catgctctac tgcaattatg
    44281 gcttcaaatg aataattgta ctcaatgtca ccttttttaa ctatgaaacc tccagtaata
    44341 tctctagttt catcagacac tttaatgcct ttttttgata gcacatcttt taatgttttt
    44401 ttatcttttt tagataatac tatctctgaa tcatctgtac aatttgattt ttcaatcatg
    44461 tttagtataa tctcttcata ttcattatct ttaagattta aaagtttgtt tttagcttct
    44521 aaaattacct cttctaaaat ctcttgtttt tttgataata tttgcttttt agcctccata
    44581 taagctcctg atatttcctt agaagccgct ttttctgctt ctgcctctgc aagctttgta
    44641 taggaagcca tttccttttc agctttttca ttagctgaat ttacttttaa atctgcttca
    44701 cttttagctt tatccaatat ttcttgtgct tcttgtttgg catcagctat aattctgtca
    44761 atcatttttt gttcattacc cataggagtg cttcctcctt ttcatttttt ttacttatga
    44821 attataattt ataattaacc aattgcaatt ccattgtaag ctaagaaaga aacaagtagt
    44881 cctaaaagag catatgtttc aactattgct gcaaatgtta tagctttacc aagttcttca
    44941 ggtctttttg caactattcc aactcctgct gctgctgctt taccttgtgc tatacctgat
    45001 actaaaccaa ctaaaccaat tggaagtcct gccataagaa gttgtaaacc ttgagctgat
    45061 gttggtattg catctccacc aataatccct gcttttgata aaataaggaa agctatgata
    45121 aatccataca gaccttgtgt accaggaaga agttgtaaaa ctagaagttg accgaattta
    45181 ctagggtctt ctgttacaac acctgctgcg gcttgacctg ctattgaagt acctttagca
    45241 gaacccatac ctgcaaataa tgctgctata gcagctcctg atattgccaa aaattgacca
    45301 tttcctaacg cgtttaaaaa ttccatattt acatctcctt tttattaatg cttgtatatt
    45361 ttgttttata cttaaacgga acaaaaggaa ctcctccgcc ttcataaaac ttattgaaaa
    45421 attctacata ctgtaaccta cttgtatgaa catatgcacc tagtgcattt ataagaaggt
    45481 ttattgtgtg tcctacaaca ccaattacaa ctgcaagtat cggaccagct atagccccta
    45541 aaaggttgat aacttgtgct ataactcctg ttgttaaaca aagcgccata attcttgtat
    45601 aagataatat atcagcaaaa taacttgtta taccatagag acttgaaaat cctttaaaaa
    45661 gcttaacagg aataccttta aagcttcttc cttgagtaag tacaagacct atagcaccta
    45721 taattgctaa atattttcct acttctgaaa atacacctat gtcccctgca acaaaaggta
    45781 ttataagtag acatacacct gttatacaca aataccaaaa acctatgtca aagattgcat
    45841 ctattacctt tccacttctt attaatgtat atgcttttat tcccaaacct acatatatat
    45901 ggataatacc gaacagtaac gacattccca taagtgtcat aacatcctct aaaggattaa
    45961 ttaatgcttt tataggaatt aaatccccca aaataccccc gaatataaga ccccatacag
    46021 tagttgaaac tccacagata ccaataagtt taattaaatt tccttcacct ttttgtatct
    46081 ttaatttata tacaacaaat ccacaaacta ctgcaattat taacccatat gcggcgtcac
    46141 ttagcatcat tccaaagaat ataacaaaga agaaagttaa aattgtattt gggtcaatat
    46201 cttttgtact tggatagctg tacatgttag ttacactttc aaaaggtgtt actaatttat
    46261 tgttttcaag cagaattggg aagccttctt ctttacttcc ttcttcagtc tgaacacaac
    46321 aatcaaactt ttctgtaatt tctttaatta attcatctgc ccttttgctt ggtaaccaac
    46381 cattaagaca gaatgttgtt tttgttttga caagtctctc tataatcttt ttttggtctc
    46441 tttctatagt gtaaaaatca tataggtttt caagcatgtg tatactatct gcatgtcttt
    46501 taatttcctc tgttttgtcc ttagaaacgt tttcctgtct aattatcatt tcattgtatt
    46561 tcttgatagc ttgtaacggt gtaccttctt catttggtaa tgaaaccact gaaaaattaa
    46621 attcttttaa aacttctaaa acatcatcaa aagtttcttt taaagtaatt aaataaacat
    46681 agttcattaa cttgtcagaa ttgacaatcc caataacact ttctggtatt tcatcttcta
    46741 attttactct taaagcatca atgtttgcgc ttatgggcac agttccaagt atagtttttg
    46801 tatacttagt ttccatattt tcaataggaa tatcaaagtt cacccatggt tctaacatac
    46861 ttttattgtt gttaagtaag tttatgttag accttattgt acctatctcc ttgtgcaaat
    46921 tgtttaactc cagagcttca gcataaactt gcccttcttt ttcactatca attttgttaa
    46981 actctctttt tggtgcaagt agggattttt tttgtttcac tagtgaagaa acaatattaa
    47041 ttgcttggtt tacatgagac attttctgct ctagcattat aacttcagat tcttcactat
    47101 cttttacaag ataatttttt aattcctcct cctcagtaag aaaggcacta tcatcaatct
    47161 gaacaaaccc acggttcata agtagcttta aaatctggct tttttgagct tctaagccaa
    47221 caatagatat tttgttcata ttaactattg ccatattatc tcactacctt tccaataacc
    47281 gcttcaatag ccttactttt tctttcttca gcagtttttt caattttcag acattcactt
    47341 ttagcattag ctaagatttt atcaacctct gctctactgt cggtgactgc cttctcaata
    47401 agagtttctt ccataagctt tgcttgtttt tttacatcag caaatatgct ttcacatttg
    47461 tttacagcat cagctctgat agactctgca tcagtaacag cttttacttt gatactgtct
    47521 gctgtctgct ctgcctcaac aatgcttttt attatttcaa aagccatcca aatcacctct
    47581 cttacctttt tactatgtat ataaccaatt agattttttt attctaatta gattactacc
    47641 tcattgttaa agtataaaca gcatttttat ataataatta atatttttca acgtttattt
    47701 cttaataaaa catattttag aatagtactt gcctagattt acatcctttt ttgacttact
    47761 tttagactat gacatgagta tggtttttaa ataccattat tttcaataat tataatttaa
    47821 agcgatatag tttgtcaata gcgttatttt gttttaaaat taacgttatg tcgacatttt
    47881 aaatttttat tttatttttt ttataatttt aatttaattt attacatata aaagtttttt
    47941 atattctttt tcaacctcta accattgaag tttattgttt tttttgaatt tttagaaata
    48001 tctcgcacta ttttatctaa cttttataaa tcatcaagct atatcgattt ttgtcacctt
    48061 atttttaatc taattttttt atatctagtt agaaataaat caatattttt aaagattgat
    48121 aaaacttaca cctagttcta atagttttat tttaaggcaa tgtattaatc ttaagtagat
    48181 aatatcatta gttactctat atattcgaca acattataaa aaaatcctcg taccaatcac
    48241 tccaatcttc aataggatat gctgctcctc catttacact tttcagtaga ttggatgtat
    48301 attcttctcc catagaaaaa agttccccta ctgtcatatc ataacctcta gatgtaacaa
    48361 ccttacaaaa tgaatccata acatcacgtt gttctcttgt ttttataagt tcctttctaa
    48421 attcatcatc attcttagct tcttctaaaa ttttagttaa tttttcattc attatgtatt
    48481 tctcctttta agtagtcttt ctgtaaactt caataagctt tatataaaaa ggaagccatc
    48541 ctaaataaac taggatgact tcgcttaact gttagcaaca catttactag tataaaataa
    48601 ttggtgtacc tggttctata ttttcatata ttttctttgc cttagaaaaa ggtgtattta
    48661 cacatccatg tgaacctgaa gacttatata tacttccacc aaaagaattt ctccaagtag
    48721 catcatgaat gcctatgttc ccattaaatg gaagccaata acttacaggt gaactgtaac
    48781 cttgaccagt taatgaaaca tttctagcct tataattaag aggatatata cctgatggtg
    48841 tagcaaatcc tctagctaaa tcacctgtta caacgtctgt tgatacaagc atttctccat
    48901 ttttaaagaa ccacatatgt tggccagaaa gactaatctc tacatatgta tttccaatat
    48961 catctttatt acgagataca gctttttgta tgtaaatagg ttctcttgta acatttttac
    49021 cagcttttac aacatcaact aatgcttcag cttctttctt tttatctatc aaccaaccat
    49081 aaattccacc tgaaattgta atatctttac ctgtggcagt tttaaatggt cttgagtcac
    49141 caaaagtact gtatttttta cttaaacctc ttgtatattc taccatagca tcattatcaa
    49201 aagttatgtc ataattcttt tctgagtcta cttttaacca tttattaatt tcagaaccat
    49261 ctaaagtttc cttacggtca ctaaaatcat atgtaacgac tacaccaaca tacttatcta
    49321 atgtcttaat agcttgtttc aacttttctg aatcctttgt gttgtttggt gcttcataaa
    49381 ctttactttc atccagagaa atagaagtat tcccagaatt aattgagttt tgtactaact
    49441 tataaaaatc atcttttttc actttgtttc caagtacctc tggtactata acataagcgt
    49501 ttttagtgct atcatactta ggataagcac tcttaggttc tacaacattt ttactatcaa
    49561 aacaagataa agcattaaac cttttttcta gtaaatcttt attatatgaa agagttatca
    49621 tattattata ctctttagat ttaaatattc caccaatcca tccaatagcg ctctgactat
    49681 cttttatctt ttgaacttta tcatctggtg taaattttaa atcaatatct tttgctttta
    49741 tttgttcttt cttattgttt ctttcctcta agtttaaagt ataggaatta gtactagatg
    49801 ctaacaaatc atttacttgc tcaactgttt ttcctgaagc ctttacgcca ttaatagtcg
    49861 ttccaaagta aaaatgattt ctaaaaaaga ttgccattcc tatataagct acaaacaaaa
    49921 cacctactac tatagctatc ttaaatttta gatttttttg tccactttct tgttttacct
    49981 cttttccatc aatcataatt aaatccttcc ttacattgta attacctatg taccttattg
    50041 gtttttaaac aaaaccatgt atgccttatt ttatataccc aaattactgt atcattattt
    50101 atatttttaa atatatagtt atttctataa aaccatttta tcttttagat aaaatgatta
    50161 ttataaatcc cccctttcaa aatcccttaa acacaatctc tgctatatta atatattacc
    50221 atacttttaa taatcgtggg actatctatc tatttttgta acactaaact actattttaa
    50281 aaaagaaact aattttgtgt tagtttcttt atcttaactt atttttatat tgaattttag
    50341 cttaacataa cgttgttata tagattctat ttaattattt tagtttctat atcttttcca
    50401 tctgcatatt ctttaagtaa ttccttagca tatccaaaat attcttcaat atcagattct
    50461 tgactaaaag aaaatataca aattaatatt tcatttgcat cttctgttat cattgctctt
    50521 tcagaatcac tagttggact cccaaattta cccactgaat cagacaatat aggaagattt
    50581 tctatattta ttaattcttt cccaataccc ttatactgtt ctccttcatc tcctacagta
    50641 aaacatactg gtgaatgtaa tttgcttaca ttatatgtac ctactggata taatgatttt
    50701 attgatatta aattgtttat ttctactata ttatttattg tataaagacc cttcccttga
    50761 ataattcttc taaccaatgc ttctgaagat gttctatatc tacttggatt ttttccaagc
    50821 tctttataag catttcttgc atctttaatt ttatctagtt tcgtaatgtc ttcaactttt
    50881 agactttgat ttatagtatc acaaaactca tttatttcat taattaattt ttctgagctt
    50941 ttatttactg ctacttttgc ttgaatgcaa cccagcttag cattaggcca tctttcctta
    51001 agtttgtcat caatacttat atctatcata cttaaacaac tcctatacac taaattttta
    51061 ttaaagtttt tatcatctat acattatagt actattcttt tgttaataac atgtttatta
    51121 gcaaaataaa tttattttta atataatttt tataaaatac aattattttt tgctttatat
    51181 tagacaatac attgataaaa taaaaaaaca ctaattattt agcaatgatt tattaaaatc
    51241 aagcattcat aatagtgctt tatataaaaa tcaaattttg ttttaaagta aatctaaaaa
    51301 attcttagct ggcattggtc gtgcaaaata aaatccttga actaaatcac atccaaattg
    51361 tttcattaac tcagcttgtt ctttactctc aacaccttct ataattacaa ctaaatctag
    51421 tgattttgct agagacacta ttgattttgc tatattgcga cttctctcat catgttcaat
    51481 gccaccaaga aaagacttat ctagcttcaa tatatcaatt ggcatatctc ttaaacaact
    51541 aatagaagaa tatcctgtac caaagtcatc catagaaata gaaaatccat acgtcctcaa
    51601 ctcatttaaa acattcttta aaatatttaa ctcattaata gccacagttt ctgttatctc
    51661 tatttcaatg aaatctgaac ttaaattata catttttatt gttttattga taaattttac
    51721 aatatctggt tgatacaact cgaccctaga taaatttact gaaactggaa ctatcttttt
    51781 accttgcttt ttccattcat ataaatcatt gcaaaccctt tcaaatacaa atctcccaat
    51841 ttttaaaatc aacttacttt tctctgctat tggaataaat actgcgggac ttataaaacc
    51901 atgttctgga tgattccatc ttattagtgc ttcactccct atcatttctc cagttacaag
    51961 tgaaaactta ggttgataat acacttcaaa ttgatttttt accaatgcta tttttatatc
    52021 gtctaaaatc attgattcct cactaagctt attcctaaca tcttcattat aaatagcata
    52081 atttatattt ttacctttag caacagacct agccatattt gctttattta cagccttttg
    52141 aatatctact tcaccttctt caacaaaata aatacccaca cacagagaaa tttcaaagac
    52201 tatactcaaa ttactttcaa tattacttcg tatattatca agttttctta taattttatt
    52261 tcttccattt ttataatcac agaaaattgc aaaataatca cttgaaagtc gtgcatatac
    52321 ctcaccttct gttaattctt cttttaaagc ttttgctatt gtaattaata aattgtcacc
    52381 aaattcatat ccaaaagtat cattaatcat cttaaagtta ttaatatcaa aatatactat
    52441 aatataattt aaagatggtt tatctaaaag atacttacta cattctctat aaaatttatt
    52501 ttgatttcct atatatgtaa ctttatcttc ataagccaaa cttaatattt ctttttcatt
    52561 agattttttt atataaatta tataaaaaat aattgcaaca aatattaata caatagataa
    52621 cactgcatat acagctctgt tgattatttg ttttgaatta ctaaatacaa cagtttctgg
    52681 aattgaaaaa attaaattcc agtcttttat ataattattt tcactattcc cattatccat
    52741 agtggcatac cctaaatatc ttctttcttc caacatattt aatacagttg cacctgtttt
    52801 attttcttgc aattcttttt ttaatctatt tacttcttga atatcattat cctgttctaa
    52861 caatttataa atattttttg ataggctatc cccttgtgga tgaaggataa ttgtaccatc
    52921 attttttaca acataagata tactattatt ttcataaaaa gatatattaa taagtttcat
    52981 cagagtattg atattgtaag tacaatataa aactccaact actttatcat tatttaataa
    53041 tggtactgaa atagcaatct tctttataga cttatttact tcatcaacat atggacttga
    53101 aacatacttc ttacctttaa ttgagttttt gaaaaattct ctatctttca ctgaaaactt
    53161 atcacctttg gtattttcag cattaccatt tatatcaact attgccatag tgtaaaattt
    53221 ttgattttcc atttcatcta aaagattttt cattttttcc tcattaggta tatctggatt
    53281 tgaagcataa tagttagaaa aaatatttaa aacatctaaa tctttttcaa tttgtctttg
    53341 tgctagttga gagccttgta ctgcaacttc ttttatatgt atttttgcct ctgacataag
    53401 aagatgtttt gtatcttcta tatagatata ccccattgtc aaaaataatg atgttaaaaa
    53461 aagaattgta attaatgtaa aacttttatt tctccctgtc attcgactcc tcccctaatt
    53521 agtcaatact tcatatagat aatatatcct ttttatacca attcatcatt tatattgtat
    53581 ataactattc ttaaatgtta cacataaaat caacataata accataaaaa cattctttta
    53641 ttattataat aacactattt aattgaattt taaattaaag aataaaaact atccattttc
    53701 tcaaacaaaa aaactcttat acaataaaac tttgttttat gtataagagt ttttatttta
    53761 tctattttat agcaaactta tcttccataa tatgaattta aatacatatc ttttatttca
    53821 ttcataagtg gatatcttgg atttgctcct gtacattggt catcaaatgc ttgaattacc
    53881 atttcatcta ttgaatccat aaacttagat tcttcaacgc caaactcttt tatagattta
    53941 ggaataccaa ctttaagttt taaatcttca atagctacca ataaactttg gaatttctct
    54001 tcatcagaat ttccttttaa tcctaaaaat gaagctattt cagcatatct ctttaatgct
    54061 tctggatatt tatattgaga gaatgctccc attttctttg gtgcatctga agaattaaat
    54121 ttcataactt ctgttatcaa aagagcattt gcaacaccat gtggtacatg gtggaaagca
    54181 cctaatttat gagccataga gtgacaaaca cccaaaaatg catttgcaaa agccatacct
    54241 gccattgtag aagcatttgc cattttttct ctagcttctg ggtcttttgc accattatca
    54301 tatgcacgtg gaagatattc aaatatactc ttaatagctt gtaaagctaa tccatttgta
    54361 taatcggttg caagcataga tacatatgct tctaatgcat gagttaaagc atctatacca
    54421 gaagcagatg ttaagctttt tggcatattc atcatcatat cagaatcaac tatagccata
    54481 tttggcatca gttcataatc tgctaatgga tattttactc ctgtatcttg gtcagttata
    54541 acagcaaatg gtgttacttc tgaaccagta cctgctgatg ttggtatagc tacaaaatta
    54601 gctttttcac ccattttagg gaatgtatat accctttttc ttatatccat aaatctcata
    54661 gctaggtctt ggaaatctac atctggatgc tcatacaatg tccacattat ctttcctgca
    54721 tccattgcac ttcctccacc tatagatatt attacatctg gattaaattc tctcatagct
    54781 ttagcgccta tcttagcaca ttccaaagtt gggtctggtt ctacttcaaa gaatgtagtg
    54841 tacttaatat tcatttcatc taataaatca gtaactgctt tagtatatcc attattgtaa
    54901 aggaatgtat ctgttacaat aaaagctctc ttcttattca ttacatcttt aagttctttt
    54961 aatgctactc ctaaacaacc ttttttgaaa taaacttttt ctggtgctct aaaccaaagc
    55021 atattttctc tcctctcagc tactgtttta atattaagta aatgcttaac tcctacattt
    55081 tcagatactg aatttccacc ccaagaccca catcctaaag taagagaagg agctagttta
    55141 aaattatata agtcccctat accaccttga gaagaaggag tattaattaa aattctacaa
    55201 gttttcatgg ctgaagtaaa cttatcaagt ttttctcttt gatttacatc atcaatatat
    55261 aatgaagatg tatgcccaaa tccaccatct tctactaatt tttctgcttt tctaactgca
    55321 tcatcaaaat cacttgcctt gtacattgca agtactggtg aaagtttctc atgagcaaaa
    55381 gcttcttcta tttcaactga ttctacttca cctattaaaa ccttagtatc tactgggact
    55441 tcaaagcctg caagttttgc tatagtacaa gctgtttgcc caactatttt agaatttaat
    55501 cctccattta ctaaaattat atttcttatt ttttctgttt catctttatc taataaataa
    55561 gcaccacgct ctttaaattc ttttttgact tcattgtata tattttctaa tactataacc
    55621 gattgttcag aagcacatat cataccattg tcaaaagtct tagatacaag aattgagttt
    55681 acagcagttt ttatatctgc actatcatca attattgctg gagtattacc tgcaccaact
    55741 ccaatagctg gttttccaga tgaatatgct gatttaacca ttccaggacc tcctgtagca
    55801 agggtcaaat ccacttcttt cataacagtt gtagttaatt ctaaagatgg ctcatctatc
    55861 catcctatta taccttcagg agctccagct aaaacagcag cctcaagaac aacttttgca
    55921 gcctctatag tagaattttt tgctcttgga tgtggagaga aaattattcc atttctagtt
    55981 tttaaagcta aaagtgcttt aaagatagca gtagaagttg gattagttgt aggtacaact
    56041 gctgcaataa cacctattgg ttcagcaatt ttagtcatac caaatgcttc gtctttctct
    56101 ataacaccac aagttttagt ttctttatat gcattatata tatactctga ggcataatga
    56161 ttctttataa ccttatcttc agcaattccc ataccagttt ctttttgtgc cattatagct
    56221 agaggaactc tttgcttatt agctgccaaa gatgctgcta aaaaaatttt atctacctgc
    56281 tcttgactat atgtagcaaa tattttttga gcttctctga tttttgctag ttttttaact
    56341 aagctttcaa cgttatttac agtatctaaa ccaactgaca taacttcatt ttctttttta
    56401 ttttctatta ttacttgttc tttatgagcc attttaacaa cctcctgaga tagtttttta
    56461 tttattatgt taatttatta acattatagc atttactttt cctattttca agttattttt
    56521 ttaacatttt attttttatc taatataaca tcatttctac taataatatc tagctttaca
    56581 aatacttaat ttaactagat atcactttat tttttacgca aatcactaat gaaatcatct
    56641 aaaaacttaa ggataacgtt atttttttaa cgtttatatt tttgtatttt ttgcaaataa
    56701 aactttataa aaagaatttt taatatgata ctctatcaag tttaaaaata aaaatccttg
    56761 aaaatgaaaa ttaaaactta attaacaagt taaagtaaat atagttaaat gtacaaacat
    56821 ttacaacata tagtataatt aaaaaaatat atattcatga tttttatata atatgaaata
    56881 aaatatattt aatattatat agataaattt gaagtttata agaaggagga taagcatgga
    56941 aaaattaaaa acaattagcg ttttttcttt gattataagt gtaattttaa cgattggagg
    57001 tattggaata gtaacttatt atgtagataa tttgtttatt agaggactat cagtatttgt
    57061 tttaattatg tcctcttctt ttgtatcaac aacagttaga ttgatatttg aagagtcaaa
    57121 acgatataaa ttctaattta aaaattggat taagtaaata attttaatat agcaaaaatt
    57181 acggagatta gctcaaaata gactttttag tccatgaggc atttttttgt atgaaattaa
    57241 atatattttc atcatttcaa caagaaaaag aggcttctct ctattttgag acagcctctt
    57301 tgctagtatt gtatctaata cataatatta taagaaccaa ttctattgat gtggactttt
    57361 tataacaggc tgtagctgtt tgttctctag agcaagttct atagttggaa ttattagctc
    57421 agtatgtgca atcatagaat taggctttgc tttgcaatta tcttgaccaa aaggtacaaa
    57481 atatatattt ttagaattaa ggagaatgcc aatatttttg aaattaaagc ttagagcatc
    57541 attcgttgat attgatatta ctagtggttt atcattacga agatgacctt ttgctgccat
    57601 taatactggt gaatctgtaa taccatttgc taattttgca gcagtatttc cagtacaagg
    57661 agctattatt ataatatcag caattccttt aggtccaaat ggctctgcct cttcaatcgt
    57721 aactataggt ttatttcctg taagttcata tgcttttttc ataaactctt cagaatttcc
    57781 aaatctacaa tcgatatttt gtgatacatc tgaaaatata gtgtgtacat tagctccttc
    57841 tttaaccaaa ttctctaact ctataaatat tttatcataa gtacaaaacg aaccagtaaa
    57901 tcctactcca attgtaagtc cttcaagtct catttctaat cccttctttc atttataata
    57961 ttttctatag cgttaacaag aatttcacca gatgtctttg gtgaatattt accaggaagc
    58021 ccaaggcaaa gagtcgcatt aatccctgct tctttaacaa cagaataatc tactcctcca
    58081 ggagaagaag ctatatctat aatagttact tcttgtgata ctttttctaa cctttctttc
    58141 gttaaaatac actctggtat ggtgttaaaa atataatcaa attgtaaaag tttttcatca
    58201 agttgactta aagaaatact ggaatatcca aatgcattag ccgtacttct tgcatcatta
    58261 tttcttgcgc caacacatac atttgcatcc aaagcgtata atttttttgc aagtatttgt
    58321 gcacatcttc cataacctaa tactaaacag ttactttgat gtatattgat tatacttctt
    58381 ttaatagctt ctgctatagc cccttcagct gtagcaactg catttaaaat agcaacatca
    58441 ttcattttca taaaatcata aatctgaata gagtgttgct cgcaaaattt acaaacttta
    58501 gttggtatgt tacctccaaa taatatttga ttaggagata aattaattag caagttatca
    58561 atagtagcat caagacaatc atccgtattt tcaatattaa cttgattctt tgtaaatgga
    58621 attggacata aaataatgga acttttggat agtgcttcag ctagtgaagt tgattttcca
    58681 acgattccat ttataatttc atttgatatc ccataagtga ctacagaata ccctttatta
    58741 attagttttt ttaccatgta cacttgtcgc aggtctcccc caataacagt tatatcgtat
    58801 tgatttgaca tattgtaagc ctcctttact atatacaata tgtttttatc aatacattgg
    58861 ttcactaaaa ttactaaata ttatacttat aacattttta ataatgctaa tctatctatc
    58921 ttttcagaag aggtatatgg aaatgaattt aaaaagataa ttttctttgg gatttcattt
    58981 ttcattagat tatctttcat tttaatcaac aaatctttct tcgtaatttt gtctatagtt
    59041 attacaaata atacaatttg atttcctctt atagtgtctg agtacggcaa tacaattgca
    59101 tcttcaattt gaggaatttt tttaacttca ttttcaatct tagtagaact aactttaaat
    59161 cctccaatat ttaaaatgtc atcacttctt ccttcaaata ttaagtatcc atcattatca
    59221 taatatccaa tatcattgac agaataaggt ttggttgcat tatatactgc atattctgta
    59281 ttcacaaaaa tttttccttc tctaatatat atatctattc ctggaaatgg tctgcctaca
    59341 cttagaggtt tttcaattag ttcatcatag cataaatatg taatataatt taattcactt
    59401 gccccatagt ataaaataat ttcagacttt ggcatatatt tttttagatt tcttgcagta
    59461 tcttcaaata ataattgaga ccctgtaaaa atacttacaa ctttgagtac tggttctttt
    59521 aagtgtttaa ccaaaagttg aagttttgta ggtataaggt atatgttagt tacattatat
    59581 tgttttatta tccttatcca tgatttacaa ttaagacctg aacttattac aatactacct
    59641 ccttcatata atactgacat tatagaattt agattccctg taaaactaag tgttccatta
    59701 acaaacaaga ttgattcccc agagatatta aaaataccat tttgtatagg aaaaaatcca
    59761 gcccaactct catatgttct atataatacc tttggaacac ttgtagaacc tgaagataaa
    59821 gctcccatac aaatactttt atctaaataa tttataaggt tcttatattg atacatatat
    59881 atattaaatt taggattaaa aatagaaaaa ttaacttttt ttgctacttc attattgtta
    59941 tcatcatata aatcaataac gtccatagat tcatcagata taatgattga aatattattt
    60001 tttaataaaa tatcatctaa tactttttta gaaagattat aatgacaaat aataggaata
    60061 ttttttgaat aattaatagc aaaaaaacta atcaactgaa acattatatt tttagaatat
    60121 attaaaattg ggatattttc acctaaagaa atttgttttc ctagttcctc actataactc
    60181 aatatatttt cataagtata tttttctcca tctataatta aaaaattttt atttttatgc
    60241 ctctcttttt ttattcttaa aagttcataa tagttcataa tctacaccct ttcaagcagt
    60301 aaagctgaac ctagaccacc agctgcagct atagagcaaa ttccatatct acccttagtc
    60361 ttttctaatg cttttaaaag atgtaatgca ataattgcac ctgaagcacc atatggatgt
    60421 ccatacgcta aagctccacc aaatatatta tatctatcta ttaactctgg atattttctt
    60481 tgaaacaaca catctataac tgcaaatgct tcattaaatt caacagcaga tacatctaaa
    60541 taattaagac tttctatctc taaaagttta tccattgctt gtatcgctga tgttggactt
    60601 aaggttgcat ctgttcctat tgtgcatgta ttaataattt ttgctttagg cttctttttt
    60661 acatgttcta aatacttctt agaacatatt ataataaatg aagctccatc attaataaga
    60721 catgaatttg ctgcatttgt aatagtttct tttcctaaaa ttgatggcat cctatctaat
    60781 aacctttgac tcatcttatc acgtatacct tcatcatagg tactattatt tataggagat
    60841 ataatatctt caagtatttt ttcctctctt gcttcttttg ctcttttatg actttccttt
    60901 acccaaaaat ccaagtctgc tttctcaatt tgatataact ctgctactct ttccgcacct
    60961 tcgagcatac tattttggct attatcatca ggagaaaatt gagcaactgt ataatttgga
    61021 ttatttgtat tatatcgctt atcattctta tgatatgtgc gcattggttg tagagaacta
    61081 ctttcaaaac cacctgcaat aattaaatca cactgaccag atttaacctt tgaaaaagct
    61141 atgtctatac tcatcatagc agaagcacac tgcatatcca cagtaaaagc aggtacttca
    61201 tttgacactc ctgctgttaa agtcattaat ctagctatat tccctcctgt cccaactgca
    61261 tttccacata taatctcatc aattttatca atttcatact ttttaattaa atcttttaaa
    61321 acacttgctc ctagtaattc aggttgaaca aattgaaaaa ttccattttt tagacctata
    61381 tgacttctca atcctcctaa tataaaaact tccttcatac attacaccct tccttatatt
    61441 atagatttat catagatttc ttctgagtac attgttcagt ttagtgccta aaaaagatgc
    61501 aataactgac tttattgtat ctccaattaa aaatggtact actgctgcaa aaagtgctgc
    61561 ttctactgtc atttgattaa aatagcacat aaatactgtt cccataaaat atataatagg
    61621 cattccaaca aaaattgtta ttagtatata tctcttaata tcattatttc gtccttttaa
    61681 taaactaata attagtgctg caaacaaaaa cccaaaataa aaaccacctg ttgggcttaa
    61741 taactttcca ataccagatg tacctccacc aaatactgga atcccaatta aacctatcat
    61801 tatatataca ccaactgtac taaatgcttg tcttggtgtt aaaatcaaag ctattaaatt
    61861 tatcattatt gtttgtgctg ttactcctgc tggagtaaat ggcaatggta tatatatgta
    61921 agatgatata cacaataatg ctacacatat tgacatttta gttaaatctt gaatatttac
    61981 tttagtattt ttcattttta taattccctt catatgtctt atttcattta gtttatatgt
    62041 acatatacaa agcttctatt aaagctttgt tttagtataa aataaagacg tttttttgtc
    62101 aactattata agcatttagg tttacattta atttataagt aaactttata ctgttacttt
    62161 atattttaat agtcatttat aaaactttgc ttaaaaacag ctctatttag actatacttg
    62221 taaattataa tatgatatat ttttatataa gattttattt tatataaaaa atagtaacaa
    62281 atagtttata cacaagatat ttaaaaggaa gtgatgatat gaaagttaat gaaaataata
    62341 taaataatat agatgaagag ttagatgaac ttagtaggaa agtttactta gagtcaccag
    62401 atttttggat aagtatttct gacaaaataa tagataaagt atttgatgaa atgttattat
    62461 tttttgcagt taaatataac tatttcaata tagtatgctt tgctattgaa aacaattatc
    62521 tggatttaaa catgccttca aaaaacaaaa aatacactaa tataatggag catttgttag
    62581 atacatcaaa acagaatgaa gataaaaaaa tttataatta tcttctaagc ttaaataaag
    62641 gtaataacct agatgaaaga ccaattttag atgaattaaa ctgtaatatt agtagtgata
    62701 cttttgaaaa aaaagatata tatcttccaa agtacttatg tccaagttgt aattctaata
    62761 tatttgatac aggttataaa gtatcaaatg acataacata taaattttct tctaaagaag
    62821 ctgaacctat tgaaatgtgt agtgaagttt cttctatcag atgttgtaat tgtgatgaat
    62881 tattagaaga tattacactt gataaactat ataacctatg caaaattcaa aactgtaata
    62941 attgtggttc taatttaact aaggtaggaa ttattgcaaa gaaaaaaatg aactttgatg
    63001 agaatactga aaaatttaaa gatgtctcaa catcttactg ttgtggaaat tgtgactctg
    63061 aaataaactt atctcaaaga gaacatttta agatttaaat atttataggc taaagtaata
    63121 agattacatt attagatact gtataagttt tttaaatctg taaaaataaa tacacttata
    63181 agaattaaaa ttgctatgaa actagttctt gatgaactaa tttcatggca attttctata
    63241 atctaataat tattaaattt taacaaacct ttattagcat tccataatta tttaaaattc
    63301 tttcaaatac cctatagttt ataggtgaaa gtatctttaa tattatatca aacttattat
    63361 ctttaaatag taaatttgtt aaatctgaaa tacaccatac aattttattt tcctcttttg
    63421 tagctatatc aattctgatt aacttacatc tataataaat atctttatct tcatatagtc
    63481 tattttaaaa attactctta aaaaatcaaa tggcaaataa attaattatc cttttaatag
    63541 aatgtaatca tattatttac tatattccat tatgtactct ttaaatcctg aatcttcatt
    63601 tacttgttct tttactatat taaaaccttt tttattataa aacacaactg cttttttatt
    63661 atctttatat actgctaagc ttagactttt atatttttta gttgcataat ctaaaagttt
    63721 acctccgata cctagatttt ggtatttggg ctttataaat aaagctccta taaagctttt
    63781 atctattatg cttataaatc cttttatttc atctccatca tcatatacaa atgtatctga
    63841 tataggtata tattcgtttt taacagtatt atagttattt tgccagtatt ctttacttat
    63901 aaagtcatgt gctttgattg tacttttttc ccatatttcc atgattttat ttatatcttt
    63961 attgtttaat ttctttatca taaattttat ttcacctcat ttttgtattg attttataat
    64021 gtttataggt aagtttattt tatttagata tgatttagtt aatataaggt ggatgtttta
    64081 gtatatgcta ttgttgattc tacaatttta tatactgctt tattaatata aaataagttt
    64141 ctaaatactc gttgatattg ctaatattct acttatctac cacttagtta atataaaaca
    64201 atgatactgt aatatctaaa gatgttacat tggcaaattt acataccact tagttaatat
    64261 aaaaccatct tcagtatcat atgctgaaat attatcaaga tttacatacc acttagttaa
    64321 tataaaacgg caatcatgtc aagtacttct aatataaaaa agttctattt acataccact
    64381 tagttaatat aaaaccttac atggaaaacg aaacatattt aatttgttct gaatttacat
    64441 accacttagt taatataaaa caaatgaagc attgagttta aatagcgata ttagaaaatt
    64501 tacataccac ttagttaata taaaacttca atgaactagg gtttaaaata cattctagcc
    64561 cgaatttaca taccacttag ttaatataaa acgaacaaat ttaatggaag caataaaaag
    64621 taatgacgat ttacatacca cttagttaat ataaaacgga ggaactaaat ttagcttact
    64681 ttgataaaga aagatttaca taccacttag ttaatataaa actcaatatc ttttcttatt
    64741 agtttcgcaa attcttcata tttacatacc acttagttaa tataaaacga aggcaaaaac
    64801 taatgactaa taaagagaaa ataaaattta cataccactt agttaatata aaacatattt
    64861 aaagcaagta cttttttaag aaaacttaat ttacatacca cttagttaat ataaaacatg
    64921 gctaagagat acagataaaa atataaatga atggtattta cataccactt agttaatata
    64981 aaactattac gagaaaaatt taaaaacgac taaaatccat atttacatac cacttagtta
    65041 atataaaact ccatttattt tatatattaa aattatagca ttcgaattta cataccactt
    65101 agttaatata aaaccttttg ttttaggatg attatataat attacaaatt tatttacata
    65161 ccacttagtt aatataaaac ttggacttat tttattgact tataaattat ctaatttatt
    65221 tacataccac ttagttaata taaaacaagc agaaataata ggactaatat ctatatcaac
    65281 caaatttaca taccacttag ttaatataaa acgatagaag aaaataatat aaaagttacc
    65341 ctaaattaat ttacatacca cttagttaat ataaaactta gaagatatag aagttcttgt
    65401 agatactgtt gagatttaca taccacttag ttaatataaa actaaattta aaataaatta
    65461 ttaaatatcc gaaaaaatta tttacatacc acttagttaa tataaaaccc caaaataggt
    65521 atatttgaat acattgaaaa cacttcaatc aagcaattat aattcaataa aaacatcaaa
    65581 atttgcagtg agcgagcagt agtgcaattg ataacaatta tcatacacct tcaatccctt
    65641 atattccaac tgttaacacc tattttagca taaatatcgc tcactgcaaa cctatataaa
    65701 catttcatca gaatacttat caatcccata acatttcttt ttaattgaat taggattttt
    65761 tatctcataa acatagattg aatcctcgct attatcaata atattttcta tttcagaaat
    65821 acatttatgt aactttcctt cagtaatatt gccttcaaaa acagaatttt gcgtccaagt
    65881 caaatatttt ctaagtatcc ttctaattct atttaacgac cttgcttcaa caatatcata
    65941 agtaacaata acaaacatac ttaccaccac acctttaaaa ctttgtacct ttcatcattt
    66001 ataaaatgtt taattaattt ataacactct aaccttataa acattctata tgaaactttt
    66061 ctatttagat ttctatgttt tatagttgtc tgcatcttat tttcaaattc ttgcaaaaat
    66121 ttcttctttc ctttttcact taaataacaa attttgtctt gatattcaaa atcattctta
    66181 ttaattcttt tattattaat caggctaaaa attattgtgt caattataat tggcttaaaa
    66241 atttctgcaa tatctaaact taaagaaaat ctctttgttg atggttcatg taaaaagctt
    66301 attgtagggt caagttgtgt cttatatatc tcacttaaaa cattcgtata cattatgcta
    66361 tttccaaaag acataagagc atttactggg tctttgggag gtcttttttc tcttttataa
    66421 aattcaaaat catcttttaa tatttgatta aatgatttat agtatgtttt tcttattctg
    66481 ccttctttac ccataagttc tgatatttct ttgacatcat tcaaatcatt tagttcatcc
    66541 tttatagtat caattaaact ttcatcaact ttgtaatatc tcatatttct taaaatatgg
    66601 tatgatgcac tttccacaaa ggattttgct aagtataatc tttcattttt atctaaatag
    66661 caggctgatt gacatactaa actgaatcct gatacatttt tcttccttgg ataatatgta
    66721 cctgaataat atccatagta attgtagaaa tgaagcatca ccccatattt gcttatgtaa
    66781 tttataagtt ttgtatttaa atctacttct ccatatatat gaatatcatc aacttgttct
    66841 actggtaata tttttttctc accttctaaa ttgtaatagt aaatcgtatt ttctttcctt
    66901 tttatatccc cattacttat aatataatag tctttataca ttgttactcc tccttcacaa
    66961 aacaaaattc ataataagca cattttgtac aataatgttt tttctcaatt ttgggaggag
    67021 ataactgcat atttatttca tttatattta taagtgtgtt ttcaataatt ctttcttttt
    67081 cttctgtcaa tatcaattct tcttgtttct tttccttaac ataatttata gtaccttttt
    67141 tctctatacc catactattc tttaaatagt ataagtaata gtataactgc atttcatctg
    67201 cttctttcat ctttgaactt atttttactt cttttacata ctcatccttt attatatcta
    67261 gcttcaagat atcatcaatc aatatttctt tatttttcat tcttttatat gaatcttcat
    67321 gtactatctt cccagacaat actctactac tattttgttc catggttatt cctttagaaa
    67381 atagccatag ttttctttta catacataat aataatttat ttttacacct tgaactttta
    67441 atacttctaa atctattggc acaaaatcac cttctttagt aaattttagc cacattgtta
    67501 atataaaaat caaataaatt ggtctatttc attacttata agcacacctt taccaaatat
    67561 ctgttttgta tgcttatttt cgtatatatc atatttatat aaaagtattt ttaaatcttc
    67621 aagacctttc acctgtatgt caattacatt ttgtttagct ttatatattg gtaaatttac
    67681 agttttttta attatctctc ttcttgcttt ttttctctta gactttattt tatttatcaa
    67741 ttgtctatca tcattactat atgcatttat aagctcttgt cccaactctt cataatcttt
    67801 gattaaagtt tcttcaattt tattatatat atcctctgga attactgtat atccttcaat
    67861 atttctaagt atattttgtg catctttaga acctaaacta tatggcgtta tagtatctaa
    67921 aatatttaaa gcactggtaa atctcttttc aaaagctgta ccttctagac tttctttaga
    67981 gtatagtatc ttaaccattt ctaccttata tttttctttc atcttcacac attcttttcc
    68041 atttataaaa gttttcagta gctcaagtcc tttttctaca atatccttat cataaatact
    68101 acctattcct gtagcttttt ctgtatatat aaatatattt gggctatttt cttcatactc
    68161 acgacttcta taacatctac caaatcgttg aaatagactg tcaagtgttg aattttctgt
    68221 atgaagctca tcaaaatcaa tatcaagaga tgcctctact aattgtgttg taatccaaat
    68281 tccatttcta tcactattgg caaattcttt tatatatttt tctaattttg ctctatcttc
    68341 ttgtatatac atagaatgta aaagatttaa gttgacatct attccttttg gtttaactat
    68401 ttcttctatt aactcatatt tttctacagc acttttaact gtatttacta ttacaagaac
    68461 ttttttattc attccacttt gtattatttt acctaagttt tcatctattg aattttctac
    68521 aatagaaaca caatgtctta ttttttctgt attacatgtc aattcagcta agttactatt
    68581 cattacacct ctttttttta attcatctat atatatggtt ggcatagtag ctgtcattat
    68641 cataaatctg ccacctatct tatgtatcat ttctatacct tttaccaata cagctgctat
    68701 ttctggtgaa tatgcttgta tctcatctat tactactttt gaatatgcta atgttgagta
    68761 cactttttca taccctctat acaaaaaagg aaatttaaat atttggtcta ttgtagaaaa
    68821 tgtcagtttg caagataata actttgctaa atctacaatc tcacttgaat tttcttgatt
    68881 actttcttct agataatcta ttgctgttga atgtaacaaa cctaagaatg tatcatttga
    68941 ttctccaaca ccaactatat tttttgctct atcaaataat gcatttatac ttactcttaa
    69001 tggaagtgta aaaaatgcct tgtctttatc tatccaaatt aaggcagttt ctgtttttcc
    69061 cattcctgta gatgcaatca gtattatatt cttatttcta ttagatttag caaatgattg
    69121 agcctctctc aaactaccaa actctttcat taaataattt tctgtctgtt ctcctatatt
    69181 tataacatta ttgcattcca caacttcatg agcagaagca ctatggtcta gtctatgtag
    69241 tattcctttt aacataatat ataaattata gtacttgtga tttttatcta ttcttttttc
    69301 tacactttgt agatatactt tactcaattt ttctgttttt attggatatc taactttaaa
    69361 ttcatgctgt agttcataaa ctttatttat taaatcttca tctaatattt tttgtattaa
    69421 atttttaaaa tctttatcta taaagatatc tctttcatga tgatatacaa taacttgatt
    69481 taatactgct ctaagttctt tatttttttt cctgtctata taactataat caataaatgc
    69541 aggagaaaga taattatggc ctacattatt ttctaaataa gttactattt taggttcatt
    69601 tattttagta tccattctgc tttttattaa ctcttgaaac ggtgaaaatg cttttccaat
    69661 atcatgaaat tctataacaa agtcaagtaa ttgccaaaat atctcctctt ctagaaaatc
    69721 taagctattt atattttttc cataactttc tcttaataca ttcatttgtt ttaaaagttc
    69781 atcagtatgt tctctaagtg tttccactgg attagattta gcatataaca taatctccct
    69841 cctaatttat attattaaaa tataataaat ctccttcatt atctaataat atatctccaa
    69901 ttgatatagt atttccatcc tcaacataat aactatctac cttattccaa accctttttt
    69961 tatcttccac agcattatta tcatagtaat tattgagtct gtaacttata ccatctatat
    70021 ctgtatcttg taaacttgtt tttggtatat atataggcat tcttatatta cattctgata
    70081 acttataatt ttcataatca tctaattgta tatcctcatc tacttcaaat ttattgactt
    70141 ctacaaatct aatatcatta attcttacta aatcttcttt tctacctaat gataagtgct
    70201 cgtcataatt taataaacta tcatatattt cttccaataa atcaaaatcc ccacctatat
    70261 gaattatcaa atttacattc aatagcatat gactattcat tggcatagat gtaatactat
    70321 cttttttata aaaatatgtt gtctggtaat tattaaatat actttcatat gttccctgta
    70381 cacttatatt aaatggtata tattctgtag catttaatat tttgtgaatt aatccattta
    70441 ttgtagaata tggaggcagt ggatatgtct ctgtaatctt catagcaaat ggttttttgt
    70501 agcatgcagt ttcttgaaat aaatctaatt tcaaaacctt catattaatt caccccataa
    70561 tattccttta cttctttaac tagttgtcca aaaaatttat ctacacttaa aaaattatct
    70621 tcaaacaatc ccttaaattc ttctttattt ataaataccc catctaccat tccaacatag
    70681 gtaaattctt ttaaatcttt tcctaaaaaa gtaccttgaa ttacttcttc tatagcttgc
    70741 ttatttattt taaatccatt tttatctcct tttaatttaa ttctacccaa gaaaaatgga
    70801 tttgctattt catatactcc tcctactaca aatagaggag ataaattttc ttgtctacct
    70861 ctaatatttc tattcaatac ttttattatt tctaaaaatt ctactactct tctacatttc
    70921 tctttattat caagttctat atctccatcc ttacctattt tagataaatc tatagtcact
    70981 gtatatgtat aataacttaa atgttgctct atgttagcaa gattaggatg ttctcctatt
    71041 ctatctgcta atcctttatt atttaaaaag tccatgtcac ttctatatgg ctcaagggaa
    71101 attgcattac ttaatcttac agtagcttct ctagtcaaag aacctccttt tttatctttc
    71161 tcatcttttt tatttgtttt catatatcca aataaatcca tttcttctga ttctcttata
    71221 cttatattgt ctttaaattg aatagtgcct ttttctttat cgacaacctg taaattccaa
    71281 ttaaacaatt ctgctgctaa tcgtactatg tcatatctta aacactggcg agatgcaaat
    71341 gtatatatat ttccatcatc tctacttaat ttttttaatt ctgatatatt acctatccct
    71401 tccccatagt ttaaactttg agcctgtgct attactgtaa atgttaaccc tcttttcatc
    71461 ttatttttcc tccttttcgt ttggtagttt atcttttcct tcatattttc cagaaattaa
    71521 tcctgttaca aatgcatacc ctacagattc aaaatctaaa tcttcttcaa caaatatctg
    71581 taaaatttct tctggtggtg ttttttcact tggtgaaaaa attcttaaaa ttgtttgcat
    71641 aaaatcattt ttattaccaa ccttaattaa atttaaaatt ttatatgtta ttccttctat
    71701 tttattttta gcattcttat caatataata atcatgtatt tcacaacctg actcataaat
    71761 ctttctcaac tttttatagt ccattttata cactcctctc ttatagttat ttaatattgt
    71821 tcttaattga acaacattga aacaagataa ccctgatatg tttgatttat tattgctttc
    71881 taaattactc ttaactatat tccttaaagt gttattaatt aaatgtttta agtctttact
    71941 ttttaaaatt aaatctaaca catttgattt aagtttataa tcatatatac tttgtatttt
    72001 tttattttct tttacaaaaa actttgctaa ataagttgga atattaaaat aatttatttt
    72061 acatttttta gaatctatac ttgctttaaa ttccacaaaa agtatattac gaagttgcca
    72121 ttcacttttt aatgtatttt cttctactat actttttata agctgattaa aaggattttc
    72181 atctttggaa tctgaatact cacttttttg agcttttaaa ctattatttc tttcaaaaag
    72241 ctcatttata caagtatcca tgttaacaaa taaataaaac tcattttctt cattaattaa
    72301 ataattcttt cttgtataag tagcccctgc tggagtacag aacaatatta gcttacaaat
    72361 atcacatatt gatggtacat acgcctgatt ccaaaacata ttcctagcat tattggtgct
    72421 tactccaagt ggagcaaaat tactttctga gtattcatct aatattccct tatataaacc
    72481 acacatttca catacttcta aactatccat atactttttt atatcttcta tgcttttatt
    72541 tttctttata aagtttttat ttatttcctt cataattttt tctatagtat ttatactaga
    72601 cttactaact tttttttcat ttatatcctt agctatacta tttaatctat catttaaata
    72661 gtttttcaac ttgtcatagt cattctcttc taataaatct tctaactctc caaaatatat
    72721 aatttgtctt aaataatcat taaacattac ctgttttaat ccatctaaat ctaatttcgc
    72781 cttagcaaca ttaaaaaaac taacttgtcc aaaataatta tcttgaacta catatttata
    72841 aagatttgct gtaagcctgt catttattga ttttatttta aatatatcta tagtctcatc
    72901 tactaaagct tctaaactat caaattcatc ataacttttt atcttagaca tactatctag
    72961 cttctctttt atcatgttaa atttttcttc atcaaatttt ttaatcttat cattttgttg
    73021 tttcacacta tctttaattt tcttaattcc atctttgatt ctatcaggct tagatttaat
    73081 aaaatttata ctgtaatcaa tatttttttt aattcttttt gatacatcat attcatccat
    73141 aaagtaatta aaataatact catgaaagtt ttcaagaaat aagctatcaa attcgatata
    73201 atcttccata ataaatattt cagcttgttt atctgctttc tttattatat ttaaaaatcc
    73261 aactataccc atattaaaaa accaatctcc tctataaact cttaacttca tttatctcac
    73321 ctccattcta aaatatctac atctccaaat ccctgagcac gtctaaaacc aattcctaga
    73381 ttgtagataa gatttaaatc atcaatatca cctttcaata taaatttacc tttgtaacca
    73441 ttaatatatt gatactcttt acctgttatt tttttaaatt ctcttaaact ctctttaact
    73501 actacctttt tgagacctat attttcaaat tctaactttc tttttaatcc attcccccta
    73561 taatttttaa ccacttcatt agttatatag tttagttctt ctatgtatct atcatcagtt
    73621 atctccaaga attttccttc tttagacttt acacagattg gagacaatgc attaaatagt
    73681 accctttctt ctgttacctt cttttctctt gataaatcga ttctaattct tgtaagttcg
    73741 tagtctttat ataaacattt tttagaagtc attaacccat tgtatatatg aaaacccaat
    73801 tctaaatctg gagtacttat atttaatatt actttatctt ctataataaa attgtcacct
    73861 tcaatactat acttttttat ataaaccgaa aatgtaaaat ttttagtttt tttattattc
    73921 ttttctttat aatagtacat gtttttatag taatcttcat tagattttct tattgcttct
    73981 tttataatac tcataaagag cgaattataa gatattggta tacaacctgt actaaattca
    74041 attttcattt tcataatatc acctctctca attaatatat taacactaga ccgtgaagta
    74101 tattggtcca agtataaatt atttgaaaaa atcagtaaat ttttttcatt ttttcaatct
    74161 ctaacaatat atcatttcta agtctatctg gttctacaac ctcaacataa gctcccatac
    74221 ttaaaatcca actttttatc tcctcataac ctctcatttt agctttaaac attatagatt
    74281 tatcatcata ttctatgatt tgttgattat taacccaaac tttctccttt ataattgttg
    74341 aaaatggatg actaattttt ataacaacat taatttcttc tcccttataa attcctatac
    74401 tatttttact atattcatcc caactaaaac ttttatctac attatatttt tcttctaaaa
    74461 ccttataatc tttaatccta cataatttaa agtctataaa cttaaatctt ttttcacaaa
    74521 aagctaccat ataagtatct gatttataat taaataaacc atatggatga acaattcttt
    74581 cactatttcc tgaatttaat gaataatatt ctatccaaaa tttgtgtttt gtggtatatg
    74641 ctctaattat ttcattacac ttattttttt cagactcata atcacaatta cgttgtgctt
    74701 gaactgtaaa ataatccata tatgtatcgc ttttttctcc tgtatttaat actgctttta
    74761 ttttttctac aatattatta aattcatttt tgtaaatatc attattatac tctaactgtg
    74821 aatttatcat atctagaatt gaaacttcac tatctaataa tttaattaat gagatgctat
    74881 tacatttatc tatttcataa ccaccataaa tcccaggtgt tgaatttata aatataccag
    74941 cttgctctaa atacatcttg taacttttta tttgcctttc acttacttcc aactcttctg
    75001 ataattcttt gcatttcatt ttgcctctgc tttgaattaa tatcatcatt ctaagtgtat
    75061 tagctaattt actaatgagt aaccccccct aataacatta attgatattt ttattattta
    75121 tttctacaca tatgtacata taacctcttt atattttata taagtagaat ttttttataa
    75181 ttttgacatt taccatatta tattttatat ttgtacaaaa ttttaatagt taatacaata
    75241 aaaaatatta ctgaaaaaat aaacatctat aattccagta atattaatta attctaaacc
    75301 ctttttatta tcatttaata atagtaacta aaaattgact aaatttaacc ttatctcttt
    75361 taaataatta tttcttacaa ttaaaaaata aactatttga accaagaaca agcttcccat
    75421 taccaaaaca gctgatgatg ctacttctat atcaaaagaa tttttcagtg cagtaagtgc
    75481 aaatattgaa tgtataacag ccactacata aggaaacaaa aatactattc caatctcaat
    75541 tgttgaagct tttttaatct cattatatgt catacccaat ttatttaact gttcatactt
    75601 tttcttatca acttggcaat ccatataaaa tttattatat aaaaaactac ttgtagtaac
    75661 aaagaaaatt aaacctataa atattgctaa aaatataaat gtagaataca ttaatttccc
    75721 agttcctatc attaattctt tagagaaaaa caaatatggt tgcatccctg actcattatt
    75781 aaatttattt tcaaattctc tgcatatctc tatagtttta cttgaatcct ctacttcaaa
    75841 tgcagtaaat ctatccacta taaattttga ttctatacta tcataaaaat tatcctttac
    75901 cacatacata tttttgtaat acgctggcat aacacattct tccacttgtt ttgccacctt
    75961 taaacctcta ttccctacaa taatattttc tttgacgttc ttatttcctg gtaaacttga
    76021 tgataataat gatatacatt cattatcttc aaaacttata ggcttaaccc ctatagtttc
    76081 tgtaatttct aagtacttag attctttcat aattctgaca ttaaaatcac ttttcttggg
    76141 aaatacagtt ttaaattcac cattaatcct tgtatagtct acattctctt tttttagatt
    76201 attttctata aacatcattc tttccttgtc tttttctttg ctatcaggct ttttagggtc
    76261 attatgcaat gtcattgttg aataatatat tgtgttagga tatattaaat taatttgacg
    76321 ctctacatct ttccaaaagg aataaactgt acctatagca gtaaatgcca ctgcagaagt
    76381 tatagttatc aagaaaaata ttctagcatt atcctttacc ttataaatca agttcgaaat
    76441 ccaaagcata tttgtatttt ttctataaaa tctcttgttt aattttagct tcttcataaa
    76501 aaatacacta aattgagaaa agaacaagta agttgctact atagtaaggg atgtaactgg
    76561 aactaagcgc agatatgttg tatattggtc ggttgaggta atagcattat aatacgctat
    76621 tataaacaaa caaatactta atatagcaag cattagtgat gttttaggct ctggttttgg
    76681 agtttttgta cctttaagta acttcaacac ctgattttct ttaactataa aagaagtaaa
    76741 tattgatgtt aatacagcta aaatcataaa atagattaaa gtagacaata tcgcattaaa
    76801 tggtatatag aattttaatg gtgataattc taaaaaagta gacataacca ctaaaaatat
    76861 ctttgcaaag attaagccta ttattattcc aattacagaa gatactatat ttattatcat
    76921 attttctata aagattaatt tcattacttg gcgctttgat atacctgtaa tataaagtat
    76981 tccaaattct ttatatctac ttttaagaaa cacactaact gaataaaaca caaagaagaa
    77041 taagaataga tatgcaatta tcttactcat cttgagacct tcttttaaat aatcaggaaa
    77101 ttgagataca tctaaatctg gatgacttgt aaacataata aaagaaaata gcagtgaaga
    77161 tgatataaca atacttgcta agtatcctaa atacgcttta atatttcttg aaatattgtt
    77221 aatagcaaac tgttttatat tcattagtta tcgcctccca ataatgtaag tacatccatt
    77281 atctcttgat aaaactgctc tctactacta cccttatata tttcattata tatagaccca
    77341 tcttttatga ataagattct actacagtag ctcgctgcta atgggtcatg agttaccatc
    77401 atagtagtaa ctttattttc tttatttatc ttttgaaata gctccattac aacctttgat
    77461 gactttgaat ctaaattacc tgttggttca tctgctaaaa gtattgatgg attgttaatc
    77521 aatgctctag caatagcagt cctctgagct tgaccacctg atacttcaaa agttcttttt
    77581 cctaataaat cttttatacc taatattgta gatacttcat tcaatctctc atcttgctct
    77641 tttacactga ccttatctaa tgtcaatggc aatactatat tttctcctat agttaatgta
    77701 tccaataaat taaaatcctg aaatacaaaa cctaactctc ttcttctaaa taatgcaagt
    77761 tcctcacctc ttaacaataa aggattcttg ccctttaatt ccatagttcc tgtagttggc
    77821 ttgtcaatag tagaaatcat atttaaaaga gtagtttttc cactaccaga tggacccata
    77881 attcctacaa attctccatc ttctatacta aaatttatgt catttaatgc gttgaaaatt
    77941 atttttttac catatacttt acttaaatta tttacagtta aaattttcat tatttaacta
    78001 tccttcctaa tttaattttt gaaataacat atatataatt gttaattata atttatctta
    78061 gtttaatttt tatagataat aaatctattt tttctataca ctaattatgt tagtaaaagc
    78121 taatactgca agtactacac ccatgattat tctgtataca gcaaacactc tcattggttt
    78181 tttctttaaa aaattcacga atttttccat aactattatt gatacaataa aagctactat
    78241 aaacccaact accaaagcaa tccacaatgt tggtgtcatt attgaataat caaattcaaa
    78301 taaatctttt cctgaagaag ccaccatagc tggtactgct aaaaagaaag aaaactcagc
    78361 tgctgtagga gaattaagtc ctgctatcca tccacccata atagtagatg cactccttga
    78421 catacctggc catgcagaca aaacttgtaa tgtcccaacc tttaaagctt gtattggagt
    78481 aattttatct atattatctg tggaatgatt cttttttcta aacaaagttt ctactactaa
    78541 taataaaatc ccacccacta taaatcctat aattacagat tgtaaattaa acaatgattt
    78601 tatattatcg taaaatagaa ctcctataat cgcaaatggt atacaaccta taattacatt
    78661 cattccaaat ctaaaaccaa tctttccttc ttttccacca gtaaatataa atttaaaaaa
    78721 ttctataacg ctatctttta tctttttcca ataaagtact actactgcta atatagcccc
    78781 aagttgtatt acaacctcaa acatttcagc aaattgcccc ttaaaatcta ttaaattacc
    78841 aaccaaaatc atatgacctg tagatgaaac tggaataaat tctgtaagcc cctctactat
    78901 agctattatg actgatttca atataaaaat aacatctaaa ctcatcaata aacatccttc
    78961 cttttttaca aaatttcttt ttttatgtta tttctcttat gttatttctt atattaattg
    79021 taatttagat tagaaaattt aactatctgc aaacattaca caaaccttac atctatgtaa
    79081 ggtttgcaaa taaactctct taaattttga ttatttaact atgaatattt catttaataa
    79141 attgtataat aaaaaagtac aattaactaa aaaccctatt gtaacagata atttacatct
    79201 ttatcaaact atttacacct ttattaaagt agattgaaac ttctgtacct tttcctactt
    79261 ctgattttat acttacttta tgacctaaat catcacatac cttttttact agataaagac
    79321 ccatacctgt agattctcca aacttacgac cattttctcc tgtaaaaaat ggggcaaata
    79381 ccctttttaa atctttagaa acaattccaa ttccttcatc tattatactt aaaattacat
    79441 atttattatt ttcttcagat tttatagtta ggtagttact ataatcttta gaatatttca
    79501 ctccatttgt aattaattga cctattataa atttcatcca ttttacatct gtataaacat
    79561 aagtaaactt gtctacttga atcttaggaa tcattccact ttttataaat aacctttttt
    79621 cttcatttac aatactagat acaatttcat ttaatttggc tttttcaact ataaagtctt
    79681 tctgaaaatt atctgccctt gcaaaatgca tagccatatc taacccttta ttcaatctat
    79741 ctatttcttt tcttatattt tcagttactt cttctccctc ataatcttgg agctgaaggc
    79801 ttataactga tattggtgtt ttcatctgat gaatccattg gttgatgaaa gttaaatgtt
    79861 cactatgtat ctttttatgc tcttgtatca ttgattgata caatttatgt tgttgtttta
    79921 aaataactga tatatttctc cccaagaatg agcttcctaa atacaaaaat gactcctcca
    79981 aagaatgtac tcctttttca aacagattat atacttctct atttttataa tacttatata
    80041 ttaaaaaaca taacaatata aaagaactaa ataaaagaat atataaactc tctccaaaac
    80101 taataaactt cataagatta cagtaaataa ttgtcataac tatgcctata taatatataa
    80161 aaatataacc tttataatct tttaaaaata acttcatact ttctccttac caattcttat
    80221 ttaatttata tcctaaccct cggacagtct gaacagcatt atctatacct aattcctcaa
    80281 atcttttcct tatcctgcta acatttacat ttaaagtatt ttcctctaca aactcaatat
    80341 catcccatat cttttctaaa agataatctc tacttactac ttttgggtat ctcttgataa
    80401 gacattcaat cagtatacct tcttttttag ttataattag attctcacca tttaattcta
    80461 gttctaacct ttctggataa aattttaatc catctagctc cactattctt tcgtctattt
    80521 taggtgaata ttcaccatat acccttctta tatgactctt tatttttgcc attactacat
    80581 catagtaaaa aggttttgtt atataatcat ctgctccact ttctaaagct ctaatttggt
    80641 ctacattacc atctctagct gatataaaaa ttataggtaa attcgactgt tgcctaatac
    80701 atctacacca gtagaaacca tcaaatttag gtaaatttac atctaataga actaagtgtg
    80761 gcttaaaact ttcaaatgta atcattatat catcaaagtt ctcttcaaca atcacttcaa
    80821 atccatattt ctcaatataa tctttaagta atgtacttat tgatatgtca tcttccacaa
    80881 tatatacttt ataactcata taattcctcc taatatacct atttactcat catataacag
    80941 tccaatttta atatattata atctttttac aatatataaa tttaatcata ttactaactt
    81001 cacaattact gacttcataa gtataaccat attattagac atcttaataa aactctataa
    81061 aaaagtatct ttttatgatt aattaatcat tattatcaac taatcattct aaaataaatt
    81121 gaaattacta attcaaaata gaaaaagtta ccttaataag gtaacttaaa cttttattat
    81181 atttcttact tagtattatt ttatcatagt ttctacttag cattatagat taattctttt
    81241 atgttcactt ttttctcttg taacttattt tttattaaga agtcttgtgt atcttcaagc
    81301 gaagatatgt ctttatctgt tatatctaaa ctaaaatcat accaagcata taattcttta
    81361 gtttcttcaa gacttaaatc aacttcttta gctacttttt ccatagcttc atcaaaatta
    81421 ttatttacat attctaaagt ttccttttca accttcataa atctttcaac tatttctgga
    81481 tgtttctctg caaagtctgt gctaacagca gttactatta taccatctac taaaccttca
    81541 ccatttgcta caagtttact acctgacttc atagctttaa gagctgctgg cccagcaatc
    81601 aatgcagcat ctacgctgcc atcagaaaga gctgctgatg cctctggaat acccatatta
    81661 acaaattcca catcatccat acttaatcct tctttatcaa gtgctgaaat caatacctga
    81721 tgtaatatag tcccttttgg gcctgccacc ttcttaccta ctaaatctgc tgctgatttt
    81781 attgaattat tattagtcaa aatcataaat ccttttggtg acctgctata agtgtttaat
    81841 atctttaact ctactccatt agatgcagct attaaagctg atgtaccacc tagtgcatgt
    81901 aagaagtcta tttctccagc agctaaagct tgtgtttgct ctggacctgt agtaatttcg
    81961 tggaagttaa cttttatatt atccttttta aattcctttc caaataaatc atcctgtttc
    82021 tgaattatag aaggaacatt taatggtgat tttacataag ttaaatttat ttctgaaagt
    82081 ttttctccat tatttttttt agcacatcca gctaaagatg ctgttgcaag tgttaatatc
    82141 gttataccta tcaatagctt ttttaatttc atttacttta ctccttaaaa tattattaat
    82201 ttctttaaat gcctttattt gacttcattg tcattaaaat atcttttttt aatttaatat
    82261 agtaatctga agttaaatct ctattataac catcttctat attaaattct tttacttttc
    82321 tttcgtctgt gaaaacaact attttcttgc ctatctttaa cgcttcatca atatcatgag
    82381 ttacaaatat cacgccttta ttagttgact tatgtattct tataacttca ttttgcatat
    82441 caatccttgt aaaataatct aatgccgaaa aaggctcgtc cataagcaac atatctggat
    82501 tgaaagaaag agctctagct atgcttactc tctgcgccat acctcctgac aattcatttg
    82561 gataagagtt tttaaatttc tgcaagttca tcatcttaag atatttttct acatcaatat
    82621 tacttttcct attatattta tttgatttgt tcaaattatc atttgtttct aagcttctaa
    82681 aattatcatt tataatttta ctcctaattc ttttattgtg tttaaatttt ctaagcacct
    82741 tgtttaaaat agtatttttt ctttgattat gaaaagctat attttcactt acgtttaacc
    82801 aaggcattaa tctactctcc tgaaaaacaa aaccaacatt aggtctatac tctttattat
    82861 cattaataaa cctaatttca ccactattta taccttcaag tgatgcaatt gctctaagca
    82921 aggtagtctt tccacaacca ctttcaccta aaattactgt tatatggttt ctatctatgt
    82981 ttaaagagat atcactaaga actacatgtt ctttattgtc aactttataa cttttatata
    83041 aatttctaat tatatatcca gagtcacata ctaaattatt catatgcttc caccatcttt
    83101 cccttactta ccttcttcac aataatcgaa aatatgtaat ctgtaattat tcctaaaagt
    83161 ccaattacga ttatccctac aataacaaca tctgttctag acatttcttt tccatcagat
    83221 atcaagtatc caagaccaga tgaagctgct acaagttctg ccccaataat tgctctaaag
    83281 ctatatccaa gagctaactt taacccaaca gcaatatcaa gaatggcatt tggtattatt
    83341 attttaaata ttatctgaag ctttgaaaac tcaaatactc tacccacttc aatcagtttt
    83401 gagtcacaat ttttaatccc tttcaatgtg cttgtaaaga ttgggaaaaa agatgctaaa
    83461 actataatta taatttttga ttcctctcct ataccaaacc ataatataag cattggtatt
    83521 aaagcaaggg gaggtgtatt ccttaaaaaa tttattagag atttaaagta ttcgtatact
    83581 ccactataaa ttccaaagaa tatccctaaa ggcacaccta tagcagtact tattgcaaaa
    83641 cctatcaaaa ctcttttcat acttgcatat acattaataa atattgaacc atcacttatc
    83701 atatttaaaa atgtactata taccttttca ggtgatggta atatataatc actccaaata
    83761 ccaagataat ttgtaatttt ccataataaa agaattataa aaaatatcag aacactcttt
    83821 actagcctta aaaacttatt attaaattta ttgaccattt acaaaccatc tccattatta
    83881 caaccatctt tacaataaaa catttctact atgtcatact tatcttcaaa accatttctt
    83941 attttttgta tttcacttaa tatttcgtct tcacttgata cttcaagaac tttttcaaaa
    84001 gtatctttga aatatcctaa tggaattggt gattcatcta tttttccaag tgattttaca
    84061 ccttcttgtt cagcaaactc tttccatgta taaaacatag cattaccctt atccttaaac
    84121 ttttcacttg caaagtctct taattgtgta catggacagg ttactaccaa catatcatct
    84181 ttacttttta tatatttatc gtataaaacc ctacaagtcc ttactaatat tggttctatc
    84241 ataggtactt caaaattatt tatcaaatta tttcttttta aaagacttat actttctgga
    84301 catctacaat ccaacattgt attttcagtc ttttttgtat actctttgta ctgctttttt
    84361 acatattcta actgtggttc acattctgtt attatgtacc ccttttttat caaatttgct
    84421 ttaataaggt gaaacttttc tccatacatt ctttctgcca ctggatttat tagaagatat
    84481 ttattcataa cgcctcctat tatttaatat tgaatattat tatcaattaa tagtttaaat
    84541 gagcacttaa attttgtcaa tatattaatt ttttcttaat aaaacaattt ttatctacat
    84601 tttttaatat tttaaatgaa atattaaata g
//
LOCUS       DAMJWS010000005        79782 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMJWS010000005 DAMJWS010000000
VERSION     DAMJWS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 79782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.005"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(242..1081)
                     /locus_tag="PC399_000348"
     CDS             complement(242..1081)
                     /locus_tag="PC399_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2591227.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1105..1863)
                     /locus_tag="PC399_000349"
     CDS             complement(1105..1863)
                     /locus_tag="PC399_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2591228.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1910..2383)
                     /locus_tag="PC399_000350"
     CDS             complement(1910..2383)
                     /locus_tag="PC399_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2591229.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSYNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2412..2828)
                     /locus_tag="PC399_000351"
     CDS             complement(2412..2828)
                     /locus_tag="PC399_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2591230.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2875..3927)
                     /locus_tag="PC399_000352"
     CDS             complement(2875..3927)
                     /locus_tag="PC399_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2591231.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4323..5003
                     /locus_tag="PC399_000353"
     CDS             4323..5003
                     /locus_tag="PC399_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2591232.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5136..5456)
                     /gene="sugE"
                     /locus_tag="PC399_000354"
     CDS             complement(5136..5456)
                     /gene="sugE"
                     /locus_tag="PC399_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF2591233.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5838..6665
                     /locus_tag="PC399_000355"
     CDS             5838..6665
                     /locus_tag="PC399_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2591234.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGSKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7487..8161
                     /locus_tag="PC399_000356"
     CDS             7487..8161
                     /locus_tag="PC399_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591235.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKGALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREKRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8149..9309
                     /locus_tag="PC399_000357"
     CDS             8149..9309
                     /locus_tag="PC399_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2591236.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASTLTTFSYILLNNLYNSIQD
                     KYLKTPINTGFITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9580..10053
                     /locus_tag="PC399_000358"
     CDS             9580..10053
                     /locus_tag="PC399_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF2591237.1"
                     /translation="MLSFTFCSLPFWICYFWCGISDIFDGLIARKLRQQSGIGAKLDS
                     IADFIFSVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYRTFSSLHTLMNK
                     ITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELNRDIKSIFM"
     gene            10339..11007
                     /locus_tag="PC399_000359"
     CDS             10339..11007
                     /locus_tag="PC399_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591238.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            11010..13478
                     /locus_tag="PC399_000360"
     CDS             11010..13478
                     /locus_tag="PC399_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591239.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGASENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTIAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTSISLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSILDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDNITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFATIFGSIAGYVSAIIINMATIEKLDFTNIPITSILQATIISILAC
                     IIATLIPLRKVKKMNIIDCIDVNL"
     gene            complement(13717..15141)
                     /locus_tag="PC399_000361"
     CDS             complement(13717..15141)
                     /locus_tag="PC399_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2591240.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15160..15852)
                     /locus_tag="PC399_000362"
     CDS             complement(15160..15852)
                     /locus_tag="PC399_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591241.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15907..16683)
                     /locus_tag="PC399_000363"
     CDS             complement(15907..16683)
                     /locus_tag="PC399_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2591242.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLFMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(16680..17450)
                     /locus_tag="PC399_000364"
     CDS             complement(16680..17450)
                     /locus_tag="PC399_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2591243.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17447..18151)
                     /locus_tag="PC399_000365"
     CDS             complement(17447..18151)
                     /locus_tag="PC399_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2591244.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18406..18918)
                     /locus_tag="PC399_000366"
     CDS             complement(18406..18918)
                     /locus_tag="PC399_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591245.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGYVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            19244..19501
                     /locus_tag="PC399_000367"
     CDS             19244..19501
                     /locus_tag="PC399_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591246.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19644..20270
                     /locus_tag="PC399_000368"
     CDS             19644..20270
                     /locus_tag="PC399_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF2591247.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20517..21296)
                     /locus_tag="PC399_000369"
     CDS             complement(20517..21296)
                     /locus_tag="PC399_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2591248.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNRCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21364..21825)
                     /locus_tag="PC399_000370"
     CDS             complement(21364..21825)
                     /locus_tag="PC399_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2591249.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21801..22478)
                     /locus_tag="PC399_000371"
     CDS             complement(21801..22478)
                     /locus_tag="PC399_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2591250.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22711..23097
                     /locus_tag="PC399_000372"
     CDS             22711..23097
                     /locus_tag="PC399_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2591251.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            23100..24899
                     /locus_tag="PC399_000373"
     CDS             23100..24899
                     /locus_tag="PC399_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF2591252.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSSNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILKDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLNKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25254..27128)
                     /locus_tag="PC399_000374"
     CDS             complement(25254..27128)
                     /locus_tag="PC399_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2591253.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27133..28797)
                     /locus_tag="PC399_000375"
     CDS             complement(27133..28797)
                     /locus_tag="PC399_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2591254.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(28811..29632)
                     /locus_tag="PC399_000376"
     CDS             complement(28811..29632)
                     /locus_tag="PC399_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2591255.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKNILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDVVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29731..30495)
                     /locus_tag="PC399_000377"
     CDS             complement(29731..30495)
                     /locus_tag="PC399_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2591256.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30492..31241)
                     /locus_tag="PC399_000378"
     CDS             complement(30492..31241)
                     /locus_tag="PC399_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2591257.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31745..32350)
                     /locus_tag="PC399_000379"
     CDS             complement(31745..32350)
                     /locus_tag="PC399_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2591258.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            32529..33167
                     /locus_tag="PC399_000380"
     CDS             32529..33167
                     /locus_tag="PC399_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2591259.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            33299..33961
                     /locus_tag="PC399_000381"
     CDS             33299..33961
                     /locus_tag="PC399_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591260.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            33961..35142
                     /locus_tag="PC399_000382"
     CDS             33961..35142
                     /locus_tag="PC399_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2591261.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(35323..37983)
                     /locus_tag="PC399_000383"
     CDS             complement(35323..37983)
                     /locus_tag="PC399_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591262.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(37980..38663)
                     /locus_tag="PC399_000384"
     CDS             complement(37980..38663)
                     /locus_tag="PC399_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591263.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(39007..39960)
                     /gene="blaCDD"
                     /locus_tag="PC399_000385"
     CDS             complement(39007..39960)
                     /gene="blaCDD"
                     /locus_tag="PC399_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2591264.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(40501..42615)
                     /locus_tag="PC399_000386"
     CDS             complement(40501..42615)
                     /locus_tag="PC399_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDF2591265.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
     gene            complement(42699..43535)
                     /locus_tag="PC399_000387"
     CDS             complement(42699..43535)
                     /locus_tag="PC399_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2591266.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            complement(44119..44481)
                     /locus_tag="PC399_000388"
     CDS             complement(44119..44481)
                     /locus_tag="PC399_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2591267.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(44715..45179)
                     /locus_tag="PC399_000389"
     CDS             complement(44715..45179)
                     /locus_tag="PC399_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2591268.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GLYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(45608..47029)
                     /gene="nhaC"
                     /locus_tag="PC399_000390"
     CDS             complement(45608..47029)
                     /gene="nhaC"
                     /locus_tag="PC399_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2591269.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(47059..48123)
                     /locus_tag="PC399_000391"
     CDS             complement(47059..48123)
                     /locus_tag="PC399_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF2591270.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(48108..49517)
                     /locus_tag="PC399_000392"
     CDS             complement(48108..49517)
                     /locus_tag="PC399_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF2591271.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(49517..51721)
                     /locus_tag="PC399_000393"
     CDS             complement(49517..51721)
                     /locus_tag="PC399_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2591272.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIIMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(51715..52089)
                     /locus_tag="PC399_000394"
     CDS             complement(51715..52089)
                     /locus_tag="PC399_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF2591273.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(52167..53597)
                     /locus_tag="PC399_000395"
     CDS             complement(52167..53597)
                     /locus_tag="PC399_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF2591274.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(53590..53898)
                     /locus_tag="PC399_000396"
     CDS             complement(53590..53898)
                     /locus_tag="PC399_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF2591275.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(54019..55086)
                     /locus_tag="PC399_000397"
     CDS             complement(54019..55086)
                     /locus_tag="PC399_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF2591276.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKINFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(55342..56715)
                     /locus_tag="PC399_000398"
     CDS             complement(55342..56715)
                     /locus_tag="PC399_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2591277.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYTLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            complement(57690..57830)
                     /locus_tag="PC399_000399"
     CDS             complement(57690..57830)
                     /locus_tag="PC399_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591278.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(58088..59248)
                     /locus_tag="PC399_000400"
     CDS             complement(58088..59248)
                     /locus_tag="PC399_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF2591279.1"
                     /translation="MKLNKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGSVSAVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSISDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(59419..59622)
                     /locus_tag="PC399_000401"
     CDS             complement(59419..59622)
                     /locus_tag="PC399_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF2591280.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(59709..60743)
                     /locus_tag="PC399_000402"
     CDS             complement(59709..60743)
                     /locus_tag="PC399_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF2591281.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTATSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKEAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSKFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            complement(61265..62626)
                     /gene="rlmD"
                     /locus_tag="PC399_000403"
     CDS             complement(61265..62626)
                     /gene="rlmD"
                     /locus_tag="PC399_000403"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2591282.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIIVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(62653..63375)
                     /locus_tag="PC399_000404"
     CDS             complement(62653..63375)
                     /locus_tag="PC399_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2591283.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(63375..63704)
                     /locus_tag="PC399_000405"
     CDS             complement(63375..63704)
                     /locus_tag="PC399_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF2591284.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            64034..64960
                     /gene="fba"
                     /locus_tag="PC399_000406"
     CDS             64034..64960
                     /gene="fba"
                     /locus_tag="PC399_000406"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF2591285.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(65095..66726)
                     /locus_tag="PC399_000407"
     CDS             complement(65095..66726)
                     /locus_tag="PC399_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2591286.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(67209..68246)
                     /locus_tag="PC399_000408"
     CDS             complement(67209..68246)
                     /locus_tag="PC399_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2591287.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(68265..69050)
                     /locus_tag="PC399_000409"
     CDS             complement(68265..69050)
                     /locus_tag="PC399_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2591288.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(69083..70216)
                     /locus_tag="PC399_000410"
     CDS             complement(69083..70216)
                     /locus_tag="PC399_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2591289.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(70264..71391)
                     /gene="hadC"
                     /locus_tag="PC399_000411"
     CDS             complement(70264..71391)
                     /gene="hadC"
                     /locus_tag="PC399_000411"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF2591290.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(71391..72617)
                     /gene="hadB"
                     /locus_tag="PC399_000412"
     CDS             complement(71391..72617)
                     /gene="hadB"
                     /locus_tag="PC399_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF2591291.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(72632..73432)
                     /gene="hadI"
                     /locus_tag="PC399_000413"
     CDS             complement(72632..73432)
                     /gene="hadI"
                     /locus_tag="PC399_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF2591292.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(73471..74670)
                     /gene="hadA"
                     /locus_tag="PC399_000414"
     CDS             complement(73471..74670)
                     /gene="hadA"
                     /locus_tag="PC399_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF2591293.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            75347..76345
                     /locus_tag="PC399_000415"
     CDS             75347..76345
                     /locus_tag="PC399_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2591294.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            76707..77234
                     /locus_tag="PC399_000416"
     CDS             76707..77234
                     /locus_tag="PC399_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF2591295.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            77261..77857
                     /locus_tag="PC399_000417"
     CDS             77261..77857
                     /locus_tag="PC399_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF2591296.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(77922..79505)
                     /gene="asnB"
                     /locus_tag="PC399_000418"
     CDS             complement(77922..79505)
                     /gene="asnB"
                     /locus_tag="PC399_000418"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2591297.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT        26430 a        13786 c         9473 g        30093 t
ORIGIN      
        1 tttatatttt tttgaaatca cttaatattt tatataaaaa tgaaaaagca cctcattctg
       61 attaaaaatc tttctgagat actttttaaa atattaaata ctacatatag cttacataag
      121 tttaaaattt aatctattat aaaattaccc taattgccaa actttttaat ataaattttc
      181 caacatccta caaatataat caatagatat agaatcctaa cttacaccaa tatatattta
      241 tttaaaatag tcctaaaata cctgtaatag ctcccactac tatcagtata aacattattt
      301 ttattggaga aagacctttt tttaaagcaa agagtactaa taaagtaagt accaaaggaa
      361 ttaacccagg aaatatctta tcgagtaaat ccgtttgaag actaaattta aaatcaccac
      421 tagtataact tattggtgta gataaattaa taaacctacc tacaagtccc cccatgacta
      481 aggtacctag tatagaagcc ccttctataa ccttatttac tattccacct tgaagtatac
      541 tttcaacggc ttttttccca tacttatatc catttgtcca cattccaaaa gaaattgaag
      601 tcattattat atattgagct attacaaata atataggtcc tgcaattgat gctcctccct
      661 cagttaaacc aatacaaaca gccaacacta taggagttat aactccttga gttaaagtat
      721 cgccaattcc cgctagtgga cccatcaagc ctgttttgat agcattcata ccatctccac
      781 ttatttcatc tgctccatta gctttttctt cttccatagc tattgttatt ccatgtataa
      841 cacatccaca tataggttct gtgttaaaaa atgctagatg agtctccact tcttgtttta
      901 actcttcctt atctggatat aattttttaa gtactggcaa cattgaatgt gaaaaagctg
      961 ttgattgcaa tctttcataa ttgtaattag attgtgcaaa gaaaaaccat cttaaccatg
     1021 attttattac atctttcttg cttaacgttt tcttagatac ttcatttttt atatctgaca
     1081 taatattttc cccctcacat gtatttaaga tgtagcacta tcttttccgt acttaattga
     1141 agtaattgta agagcaagta ttactccaat aacagatatt actattatat ctactttaaa
     1201 ataaacagcc attagtattc ctaatatgaa aaagggcata gtaaacttgt ttccaatcaa
     1261 ttttaaattc atagcaatcc caagtgctgg caacatagcc ccaattacat taaatacatg
     1321 taacacatta tcattcatta cttccaacat accatctatc gcatttggac caaaataaac
     1381 aactaataat gctggtataa aactcattgc aaataacagt atctgtggtg gtactatatt
     1441 tataaatgct actctatcaa cattcccttt ttgaacctct ctatcagccc aatgtgcaaa
     1501 aaaagaattt actgtcatct ttccaatcca aagtacagtt gcaacagttc ccaatggtac
     1561 agctattgcc attgcagtac ttacatctag cttagatgct atagctaaag cagtacctac
     1621 atatcctgca aaagctgggt cccccatctg agctccccct gctgatataa aacctagata
     1681 tatcatattt atacctgctc ctataattgc tccttgaaca gggtctccta atataattcc
     1741 cgttacaaat ccagccaata gaggtctatt ccatgtaaaa aatccaacat aagtcaacca
     1801 cggactccaa gaaagataat aaaaaattgc tattaatact gcttgaatta gtgaaatgtg
     1861 catattcttc ctccctattt tcttttaaag atttacaaga tagtcttctt tacaataatg
     1921 ttcctacatc aattttcttt gcatctggta ctatttgtat aaagcattta acatcttttg
     1981 atattagctc tctaaatatt tctttttctt catctgacac agatatattt ttataaaaac
     2041 tttttctacc aagccctgct cccatacctc caacattcaa ttcctttaaa tctaccccgc
     2101 tatttactaa acctaagaca gttttagggt atttgaccaa tactattaaa ttttcatctc
     2161 cattatatga ttttaagact tcagcacctg attctacatc atgtacttca accttaacag
     2221 ttgatggagc tgccatttgt aaaacttgaa ctataaatgg gtcctttact actaaatcat
     2281 ccacgattac tatccttttt gctgatgtaa tcttacacca agcagtaata acttgcccat
     2341 gtattaatct atcatcaatt cttgttaatg ctatattact cataattatt cctccagatt
     2401 tttaatttat tttacaactt taaaacagtt ttaacatctt tgattcctga tatcccaact
     2461 tcctcacata aattcttcaa gtcttttaac ttcatagtat ctctgtttaa aaataactct
     2521 aaaagcatag gtaaatttac acctgtcaaa cattctaact cacatacctt agcaaattta
     2581 tttattataa tagcagatgt attaaatgga gttccgccat aaaaatcaag caatactaaa
     2641 accccttcat ccttatattt ctcaattcca tcttctaact ttctactaaa ctcatctatg
     2701 ctgtcaccat ggtttaatcc taaagtttct acattttcct gtttgcctac tattaattca
     2761 gcactttcta acattgcttt agccatattt ccatgtgtgg ctataattat tcctgccata
     2821 tgaatcatac ctttcttaac cttttttata tggattatta tttagtactc taatctaatt
     2881 atctatatta atcattatct taccaaagaa aatatccctt ttgtatactt tttcaaaata
     2941 agctggtact tcttctaaat ttattctatg tgttactaat ttttcaacat tcatgtcacc
     3001 cttttgcata tgatacaatc cagcactcca ttcttttcct gggaatggaa aggagtttcc
     3061 aaaccaagtt ccctttacag ttaattcact tcttactatc ttttcaaatt gctctctagt
     3121 caaagctaca tccccatatg gtacacctgc atacaatact gtacctccct ttttagctag
     3181 caacagtact tgaccacatg tcagtggagt accagccgat tcaataacaa tatctgcacc
     3241 atctccatca gttaaccttt ttatttcttc tacaatattc ttttcttttg catttataca
     3301 aatatctgcc cctaactctt ttgccaagtc taacttctca tcaaatacat ccacagctat
     3361 tatttttgta gaaccaaata tttttgccca ttgtattgaa aataatccta ttggacctgt
     3421 accaagtact acaacagtat ctccaacttt agcttctgac ctaaaaagac catgtcctgc
     3481 tatacatact ggttctagcg cagcggcagt ctcataactt atctcatctg gtatttttat
     3541 taaatttctc tctttcacct ttgtatactc tgcaaaacaa cctccaagtt ctttatttcc
     3601 tattattgct acattattac atcttgaata caagcctttt ttacattcat cacattcaaa
     3661 acatggcata gctggacaaa cagcaactct atctcctatt ttaaaactcc ttacttcttt
     3721 accaacctgt gccacttctc cagaaaattc atgccctaaa atttctccaa ccatatgtgg
     3781 acctgtcttt gaatattttg atatatcaga accacatatt ccagctactt ttactttaat
     3841 tattacatca tctttttcta atatttttgg tacatccaca tcctccactc ttgtgtctct
     3901 tataccatag aatctaactg acttcactat taaccacccc tactttttat ttgagaatat
     3961 atccctattg tttatatgtt aaatatctaa agcaactttc atgccaatat atgtaagcat
     4021 tgtattcatg cattttataa aattatcatt aaatgtgtgt caaagtgtgt caaaagtttc
     4081 ttatattaaa acttatgtgt gtcaatataa acgacactaa cattttatat tagttctctt
     4141 attataagtt ttataaaatg tacttttttt gttattttta taaaattgtt cggaaatttc
     4201 ccttctattt gtgatataat attaaatata aggtatcaat ataaattttc atgcgaatat
     4261 cagaactttt ttgacactaa tgccttattc aatataataa tttaactttt tggaggttgt
     4321 ttatgaaaaa agtgtatcaa ggaaagacta aagatgtttt tgaattagat aatggaaatt
     4381 acttacttaa atttaaagac gatgtaactg gtaaagatgg agtatttgac ccaggcgaaa
     4441 atagtgtagg tctaagtata gatggaattg gtagagctaa tttagagaca tctgttaagt
     4501 tctttgaaat tctaaacaat gctggaatta aaactcatta tgtaagtgct aatttagaag
     4561 aagcaactat ggaagttcta ccagcaaaag tttttggtaa tggattagaa gttatttgtc
     4621 gttatagagc tgttggtagt ttcttacgtc gttatggttc atgtgttgaa gaaggtgcta
     4681 aactagattg ttacgtagaa gcaactttaa aagatgatga ccgttgtgac cctcttataa
     4741 catctgaagg tttagaagct ttaaacatta tgactcaagc tcaatttgat tcaatgaaaa
     4801 gcatgactca aaaaatttct aacatagttc gcgatactat agctgaaaaa ggtcttgagc
     4861 tttatgatat taaatttgaa tttggattct acaatgatga agtaatctta atagatgaaa
     4921 tagcatctgg aaatatgcgt gtttataaag atggtgtaat agttgaccct atggatttaa
     4981 ctgctctttt attagataaa tagtatatac atataattta caaatataat aaacttataa
     5041 ataagataag ctatgaagta aagacttttt aaatgttgat tatttttaaa cattttctcg
     5101 cctttaattt catagcttat ttaaattaat atactctaat tagtagataa tagctttaac
     5161 cctacaattc ccaccacaat aaaacctata catgcaattc ttgatatact aataggctca
     5221 tttaaaatag ccatgcctac aatagctgta ccgacggtac caattccagt ccatattgca
     5281 tatgaagttc caagtggaag atgttttagt gctaatgaca aaaaataaaa acttacaatc
     5341 attccaacaa cagtaagtat acttggagtt aatttagaaa atccatgtga attttttagt
     5401 tgaaaagccc aaaacacttc aaacaaacct gcaacaaaca aaaataacca tttcataaat
     5461 tcatccctct tttcaaatat gtctcttatt tttccaataa aataacctga gaaatattat
     5521 aaaatatctc ccaggttttt gtccttccgt gttcacaaca aaaactatgt tgtgggtttt
     5581 atctcggacc agaccaatca ttaataattg cggaacccta taaaactcta ttaattattc
     5641 tattttatct ataataatac tatcaacata tataaaaaaa gtcaatatat gggttccata
     5701 tataataaaa ataactttta tatacctttt aatattctta ttcacttctc tccataccca
     5761 tttgggtcaa tctaagaata tctaattttt atttaatatg ttataataac ataaaaatat
     5821 tgaaaggagt aatttctatg aatttctata aaattgcagt atgtcagatg attactacag
     5881 aaaataaaat tgaaaatatc aatcatgctg tagatatggt aacagaagct gccatcaatg
     5941 gatctaaaat tgtagttttg ccagaaatgt ttaactgtcc atatgaaaat aaatattttc
     6001 ctaaatttgc tgaagaatat ccaggtgaaa caacaacaat attgagtaaa ttagctgaga
     6061 aacatggaat ttatctagtt tctggtagta ttcctgagtt agaagatgga aaaatctata
     6121 atacttgcta tgtatttgat aaaaatggag ctctaattgg aaaacatcgt aaaatgcact
     6181 tatttgacat tgaagttaca ggaaaagtta gttttaaaga atcagatact cttacagctg
     6241 gaaatgatgt tactgtaata gataccgaat atggaaaagt tggtatagct atttgttatg
     6301 atattcgttt ccctgagtta tcacgtttaa tggctcttaa aggtgctgaa atcgttatct
     6361 tgcctgctgc atttaatatg actactggtc ccgcccattg ggagttatct ataagaatgc
     6421 gtgctttaga taatcagata ttctatgttg gtgctgcacc tgctagaaat atgaatgctt
     6481 cctacatagc ttttggaaat tcaagaattt cagacccttg gggaagaata attgctcaag
     6541 ctgatgaaaa agaatgtatt atttatgctg acattgatag agatttaatt cctgacataa
     6601 gacaacagtt accacttctt aagcacagaa gaactgattt atatgaactt aatactttga
     6661 aataaaacag aacataaccc cataaatcat aaattaagtg ttaaaaaaat ttatggggtt
     6721 ctcattaatt tgatttaaaa ttaatttcat aaaaataata ctaatattaa tttaattaaa
     6781 taatactaat aattactaat gattatgcta attatcagca tttactaatt taatcactat
     6841 tagttatttc ttttattaaa tatctttact ttttttactt attatgtctt ctagctttac
     6901 atttggatta tagtatttct ttctattttt agtcacatct ttctattgaa ttacttgctt
     6961 aaaacaccaa tgcacacatt ccagacaatg ttcacaatta tgtaaaaatc ttgctctacc
     7021 ttcttcaatg ttgatattat ctctcctagt tgatattatc tttcctaaaa tttcttttaa
     7081 ttaaaaaaca acattcgttg atatatcgag tatattttaa gcaactgaaa aaaacaataa
     7141 taattgatat atcaactaat taatgtagat taattcattt ttataattta tatacttttt
     7201 aattaatcta cctttttaat taaaattctt cctttctatt tatccatttt aaacttaaat
     7261 tataagattt catcagctgt gtttttgcat tccaataagt attaaatatc aaatatatat
     7321 tctattaaaa tttctaataa tttcatcctt gatttattta atatataaaa atatactaac
     7381 tttaatgatt aagttttgaa atataactaa gaatttgaga ttttattgag atttttatac
     7441 taaagtatac atactataca agcttaattt agaagggagc attcttatga acaataaaat
     7501 actcgtcgta gatgatgagg aaggtataat aacactctta aaagattact ttgaaatgaa
     7561 tggttatgag gtttacactg ctcaaagtgg aaaaggcgca ttaaaaaaaa tatccaaaaa
     7621 tccagatatt attctacttg atataaacat gcctgaaata gatggaattg aagtttgtaa
     7681 tagaattaga gattatgtat catgccctat tctatttctt acagctagaa ttgaaaatac
     7741 tgacaagata aatggttttc gtgctggtgc tgatgattat attgtaaaac cttttgattt
     7801 agacgaactt ggagctagag tatctgctca tttaagacgt gaaaaaagga agtcaaatca
     7861 aactaaaatt aaattttata atgatttaac gatagattac gaaaaaaggt taatttgtat
     7921 agataataat cagatttcac tttcaaaaaa ggaatttgat atagtagaac ttctctctat
     7981 gaatcctgga caagtatttg ataaagaaaa aatctatgat agagtctggg gatacaatag
     8041 caatggaaat agtgatgtta taatggaaca cattagaaag atacgaatca aactatcaaa
     8101 acatactctg gaaaattata tagaaacagt ctggggagtt ggttataaat ggataaaata
     8161 aataatatgt cattaaaaaa agctttcttt gttcttactt tatcttcact aattatagca
     8221 tcaacactta ctactttctc atacattttg cttaataatc tatataattc tatacaagat
     8281 aagtatttaa aaacacctat aaatacgggt tttataacca tagttcaaaa caatgtgaac
     8341 caatttagtg attatgataa aacgttactt aatataatca acatacttca gcttgtactt
     8401 ccacttatat tttttatagg acttttatta ttagctgata taatttttta caaggtaaaa
     8461 ttaaaaacac ccatagaaat actcaataaa ggtgcaatgg aaatatccaa caacaattta
     8521 gacttttgct tagagtataa taataatgat gaattaggta atctatgtaa tgcttttgaa
     8581 aaaatgaggt ctgaactaaa taaaaacaac ataaagatgt ggactatgat taatgataga
     8641 aaacaactta atgctgcttt ttctcatgat ttaagaaatc ccctaacagt tctaaaaggt
     8701 tactcaaact atttaacaaa atacatacca acaggtaagc ttagtgatga gaagatttta
     8761 tcaaccacac aacttatgtc tgagcatatt aacagaattg aatactatgt agaaaatatg
     8821 agtaatgctc aacgattaga agatttagtt gtttcaaaat ctatgtcgaa tataaacaag
     8881 tttatagaaa acttagatga aaatatatct atcattgcta aacaagaagg aaaatctttt
     8941 acacttaaaa gtcaaataaa taatataaat ctattttttg atgaaaatat cattcttaga
     9001 gtcgtcgaaa atataatatc gaatgctttt agatatgcta gaaataatgt atccatactt
     9061 atatatcttg aacaagaact ccttaccttt gtaattgaag atgatggtat aggatttagt
     9121 aaggaatctc taaaattagc tttaagacct ttctatagag ataagacttt aaatgacagc
     9181 aatgcacact ttggtatggg tctttatatc tcaaaaatat tatgtgaaaa acatggtggc
     9241 tctatttcta ttgaaaataa ttctactaac agtgctaaaa ttactgctaa attctcaaca
     9301 agaaattaaa atgattttgt aagctttcac tgctattata taaaatttgt tagtttagaa
     9361 tttataaaat gtatttcaat ttataaccaa tatatatact attttgtaga aattgtaaga
     9421 gttgcttcaa ttataatatt ttggcaaaag gcatttgaaa caataataga aatatatatt
     9481 taaatatgta gaatagtatg aaattagaat tttggaggtg attatattaa aagttttgct
     9541 aataatatta cattctctcg tattgtgttt gcattgctaa tgcttagttt tacattctgt
     9601 tcactgcctt tctggatatg ttatttttgg tgtggaatta gtgatatatt tgatggtctt
     9661 attgcccgaa agttgagaca acaaagtgga ataggtgcaa agttagatag tattgctgat
     9721 ttcatttttt cggttacaat tttggttact gtcggaaaaa atattgctct tcccatgtat
     9781 ctatggtggt gtattgttgc aatagcattg ttacggttga ttagttacag tattggtttt
     9841 tttaagtatc gcacattttc atcgcttcat actttgatga ataaaatcac tggaatttct
     9901 atctttgcgt ttccattact ctatatttgc tttggtcttg atgttgcagg aattatgata
     9961 tgtatcgtgg cttttctttc ttcttttgaa gaaatagcaa ttatgataaa atcaaaagaa
    10021 ttaaatcgtg atataaaaag tatatttatg tgaaaactta gaatttgtga caaaaataat
    10081 ttatataata atgaggaagt aaaacataaa aaattatcca caagtttatg tgtaaaatag
    10141 ttttaaagca atagagttgt attgtaaagc ctttagtgca taaattacat tttaaattaa
    10201 aaatgatact gaaactgctt atcagcactg cgaattatcc gcaaatagtg aagacttttt
    10261 agatttaagc taaaagttgg gaaaaaattg agttttaaaa cttacactct tcttatatag
    10321 aaaaggagag tgttttttat gattataaaa gcaaaacaat tatcaaaaat atatggttct
    10381 aataacaata aagttatcgc acttaataat gtaaatttag aaataaactc aggtgaattt
    10441 gtttctgtaa ttggaccttc tggaagtgga aaaagcacac ttttacatat tttaagtgga
    10501 ttagataatc ctacctctgg acaagtgtta cttgatgata aagatatata taaacacact
    10561 gaaaaagaac tatctgcact tagaagaaaa tcctttggat tcgtctttca acaatttaat
    10621 cttttacctg ttctgacagc atctgaaaat atttcaatgc ctgtacttct agataaaaaa
    10681 caacctgata aagaatattt aaatgaaatc tcatcattac ttggaattgc agatagactc
    10741 aatcatcttc cccatgaact ttcaggtggt caacaacaaa gagttgcaat tgctagagca
    10801 cttattgcaa aaccagatat aatatttgct gatgagccta caggaaactt ggacagtaaa
    10861 agtggcagtg aagttatgaa tcttcttata aaaacttcaa aacagtttgg aaagacttta
    10921 gtggtaatta ctcatgatga tagaattgca aaacttgctg atagaaaaat ttcaattatt
    10981 gatggtgttc ttatggaggt gaaatagaaa tgaagaatta cctttcactt tcaataaaag
    11041 aattaaaaag ccagaaactt atgactacat tcatcattat agcaatagta ctatcaacta
    11101 ttatgacaac tgtggttgga cagtcaattg gtatcctaca aaatttaaga attgaacaag
    11161 cccgttcttt taacggtgat agacatgtat cctttcacca acttactaaa aatcaagttg
    11221 atgacataaa gaaagatgat agagtttatc aagctggtac ttcaataact cttggcagta
    11281 gtaaaataaa ggattctggt atctctgtac ttgtaaaaga gtatgataaa acaggacttt
    11341 ccaactaccc caaattaatg aaattaaaat ctggaaattt gcctaaagat aaaaacgaaa
    11401 tagccttaga tgaaaacaca ctaaagctta tgggtataaa accaagacta ggagtgacta
    11461 tacctatgaa tttagatatt tcacttttaa atgatacaat accaccatat aattacacag
    11521 ctaactttaa attgagtggt attttagagg atgattatac aggatatgta agtggtatag
    11581 taaacggtat tgtaggcaaa ggtgcatcag aaaatctatt acctaagaga tatattttat
    11641 cctctttgga ttttaaaata aaacagcaag agaagtttca agaaatagta aatcaattag
    11701 ctaaaaaaat aaatctttct cacaattcaa ttcaatataa ttggatttac ttaaatgctc
    11761 ttaaaataca gtttgaaaaa gatgaaaata gttctaatag tgatggtata tctatgatta
    11821 tattggtatc tttatttgtt gccttattgg ttttactggc ttctggactt gtaatctaca
    11881 atattttaaa aatctctgtt acaaagaaaa ttaaggagta tggatgttta agagcaatag
    11941 gagctgaacc aaaccagata tataagatag ttattttaca aatcttaatt ttatgtacta
    12001 ttgccatacc tattggagct gtaattggaa ttatatcttc taaaggcata actggaatgg
    12061 tcactaatat cttaaatcca gatatacttc tagcaaatga taataaagaa attactgagc
    12121 ttatacataa aaacacaaca gcatatatat tcccattagt attaagcact agtatatctt
    12181 tgattttttc acttatttca gcattaccat cagcaatata tgcttcacat gtctctccca
    12241 aaattgctat ggctggcagt actaccaaaa tcaaaagaaa aattaaacga gaaaaaacaa
    12301 taaaaaactt tgaaagacat ctagcctggc taaacttaaa aagaaataaa ggaagaacta
    12361 taataaccat actatcatta tttatgagta taacagtatt cgttgcactg agtgagttct
    12421 ctaatgtatt agatgtatct aggtcagtaa gtaatttaaa agaaggagat ttttccttaa
    12481 caaatgaaat atctggtttt gataagtcaa tactagataa aatagataaa atgaaaaacg
    12541 tcaatcgcac ttcctttata aagtactctg aatacaaaca gggcgaaata gatacagata
    12601 taaattttga aaatagtggt gagatgttaa aaatcattgg aattgatgag caaactttaa
    12661 aagacttaat gccttctatt acagattcta tactagaaga ctttaaaaat ggttctatct
    12721 gttttataaa aaatccgatt gctatttcta caccaggtgt aaaaactaga catacaaatt
    12781 taaaacctaa agataatatc acaattaaca aaaaacaact agatatatat agtacagtag
    12841 ataaaatgtt ctttttacaa ggtaatggct ggataaatgg tgttgatgtt attgtatatg
    12901 attctgttta taacaccttg acaaataaaa acaagttcaa ccagataaat atttatgcta
    12961 aagataaatc aaaattagaa caaataagat tgtctattga acagatttgt gaaaataatc
    13021 ctggctctca ttggatatca tatattgaat cagataaaca acttaaagag agttttaaac
    13081 aaatagaatt tctagcatgg gcagttatat tatttgtggg attgattggt actttaaaca
    13141 tcattaacac aactcataca aatataaata ctaggactaa tgagattgga gttaaaagag
    13201 ctattggtat gagtaatagt agcttatata agatgttttt atgggaaggt gtttattatg
    13261 gaatttttgc tactatattt ggttcaattg ctgggtatgt cagtgctatt attataaaca
    13321 tggcaactat agaaaagctt gattttacta atataccaat tacttcaatt ttacaagcta
    13381 ctattatttc tatacttgca tgtattatag ctactcttat tccgttaaga aaagttaaaa
    13441 agatgaatat aattgactgt atagatgtca atttataact attttacaga taataaataa
    13501 attaaattat caatgactgt ctctattttc ttttaaatct aactaataaa tctttttaac
    13561 tataatataa gctttaaaaa aggcctgtct caaaatagag ataagccttt tttcattgtc
    13621 gaaataatga aaatatattt aacttcacaa aaaaagagtg cctcacgaat taaaaatttc
    13681 gctttgagac actccccttt aatttaaaat atatagttaa atgctatgcc aaaatttcac
    13741 acatgctcca cctgattttg aattttctat tttaacagaa cctccaagct gttccactaa
    13801 ttgttttaca atgtaaagcc caagaccaga atgtcctcct ttagtctgcc tagcttcatc
    13861 tcctcgataa aacttatcaa gagccttttt taaatcttta gagctaaacc ctctaccaga
    13921 atcacaaatc tcataagaaa tacaattttt ttcatccttt acagtaatac taatattacc
    13981 acctgatgga gtgtattgca gactgttgga tatgatatta tcaaaaattc gtgtcaacct
    14041 atccacgtcg atttgaattg gggattgtat attcccctgc attttcaata taagttcaac
    14101 ttccttttga tgagcctgta attcatagtc ttgtaccttt tgcctcaaaa attcaggtaa
    14161 atttatagga acaagattta attgtatatt tgacttttct aagtcagacg tatattgcat
    14221 cttttggaca agagcagcac ttttttctgt attttctctt atcactgtaa gatacctatg
    14281 aagtttttca ttgtcatctg tattattacc aataagtgca tctgtatatc ccaaaataat
    14341 agaaagagga gattttaaat catgtgctag agcttccacc atttcaacac gttcctgctc
    14401 catcttccac tgtgcagata aagagccttt taattcgtct ttcatttcag aaaatgcact
    14461 acaaagttta ccgagttcat tatctgaata atagtcgatt tcaaaatcaa ggtccttttc
    14521 ttttatcttc ttggacgcat caatcaatag atgtaatggt tgattgatat tctttacaaa
    14581 gcgcttagaa agccatctag taaaacccac gatatataga aatggagaaa acagagccat
    14641 tataataact gcaaatacaa atctccctct aatatttgca aaggtgattt tcacttggta
    14701 gaatagaata acagctccct caatctttcc attatcccct ttgattggaa cagtatggat
    14761 ataaccacct ttacgaacag ttttattgat aaaattatta aataattctt cttttgtttt
    14821 aaatagtttt ttggggttag taccatataa aatattgcca ttattatcca caacttgata
    14881 tagcatatca tccccacgaa tcgttctttt taaaccttct tcattcgatt gtgataacaa
    14941 agttatgttt ttttcattga tgtatttttc aattctagga acttgtcgct cataatagtt
    15001 tggtggatag atatccttat ttaaggaatg aatatacaaa tagagagcaa atacataagt
    15061 aatcactgtg gcaactatac tggctattat aataaaaata aaagtatgtc taaattgggt
    15121 aataagggat tgatttttca tttaataacc tctctctctt tacattttat tccacttata
    15181 cccaataccc caaactgtgg aaatatattc tttttctggt gtcacagcag aaaacttagc
    15241 tcgtattttt tttactcgtt caacaacagt tgaggaatca ccttcagaat cacaccccca
    15301 tactttttca tagatttgtt ctcttgagaa aacttgtcct gcatggagtg ctagtaattc
    15361 aacaatatca tactctattt ttgttaaatc aatattctta ccatcaattt taattgcacg
    15421 ttccttcata tataagcata acttcccaaa atacaaagta gatctcttgt tctcttcatt
    15481 gatatactgt gaacgctttt ccctacgcag attagcttca atccttgcca taagttcacg
    15541 cagtccaaat ggctttgtta cataatcatc ccctccaaag gtcaaccctt tgattttgtc
    15601 ggtttctgat tgctttgcac ttaaaaatat aattgggcat aaaacatcat ctctaattgc
    15661 ctgacatacc tcaaatccat tcattttagg catcataata tccagtataa taaggtcagg
    15721 ctcctgtttt gataactcta tacctgcctg accatcataa gctatcaaca cttcatgtcc
    15781 ttttgccttc aattcatcct caagtagcat tactaggtcc atttcatcat caataatcaa
    15841 tattttactc aatataacac cacctttctc ttatgggaag gtcagtatta aaacaatatt
    15901 cgtctactaa taacttttcc ttccctccca cattttaaac caaataacgc ctcctatcaa
    15961 gaatataatc aaacctattg ctactactat aagctctata ctagctgttt gcatcacttt
    16021 agagtaaaat gcctcagttg aaatagttcc agcttcttta ataaaataaa ttcctggaag
    16081 catacctagt tttactggcc atgtccatgg aacaaatgcc catacttttt caccaacgct
    16141 agttaatcca agtattgctg ccatcaaaat tccaccgata ctaataccaa tagatgcacc
    16201 cataccccat gcaaaactca cccaaagatg aatagctaat agtggaagtg tcccaatcac
    16261 cacaagtcct gctgctgaca ggaataacca tattgttgct ccagatggca ctgcaatact
    16321 cattcctatg caaagtatca acgtagcaat aaatgtgcat atagttaggc aaaacagaag
    16381 aaaaagaaat tttcccagat ataatctaac acgagaaata cctacacata aaaaaccatt
    16441 aaaatttcca gcaagctctt cctcctgcac tataaatcct gctaaaatac ctactccaat
    16501 aggaatgatg aatacagtcc atactgtaaa aaatccttca aaagcaaatt cttgtgtact
    16561 tccagattta gttgtgagat aaaaaaccac acataatgaa aaaataatag gcataaaaag
    16621 agtaagccac tggacagctg tcctctttgc tcgaagccat tctgatgata atagctttat
    16681 catttgtcat ccctcctatt gaaccaagct gcagtaagtg ctgttaatac tataaacaca
    16741 accaaagaaa caacaatccc aataggaatc actgaagtat tcaaaagtgg gtctccagct
    16801 tctaatatag taccgttagg atgtatgttg atgattgggc ataccattct aactgcccaa
    16861 ctccaaggta ctgcaatcca aaatggtttt gtcgcagcaa ggacaccaaa tatcatacct
    16921 aaagctccaa ctcccatact taggaacata cctttccatg tagctagcca tagctgaagt
    16981 ggaagtatag caagagaaac aacccagcat acaatacttg cccctataat ctgtccaaaa
    17041 ggaactggac cagctttcaa tattaaacca gttataattg ttacgactat taaaactagc
    17101 gttgaaataa aactgtagac tgccatagct ataactttat taatccaaag tgtcatagga
    17161 gacacattgt gtgtacgtaa agcacgataa tttccagctt ttttttcctg gaaagctacc
    17221 aaagtagcaa acaatgcaaa tccaataggc agaaataaaa atgaccacca gttaaaaatt
    17281 aatgctaaaa gactttgcca cgcccaagaa gttgtttgag ataatggatt ttgcatgagt
    17341 gttgattgca ttataaatgc atatgctgca aaagacactg ggaaaaaaac aataagttta
    17401 cccataaatg ttcttttata ctttaaaagt tctgattgta aagcattcat ctgttttgtc
    17461 ctcctttaag aataacatct gtaaaaaatt cttctagatt ttcattaggg tcaggcattc
    17521 cttgaaataa tagtcgtcct ccatttataa ttcctatatt atctacaact tgagcaacct
    17581 ctgatagaat atggctagaa agaattacag ttataccttt ttttggaaaa gaagcaatca
    17641 attcacgcaa ttcttgaatg ccaaaggggt caagaccatt ggttggttca tctagtatta
    17701 acaactctgg gtcactcaaa agtgcaatag ctattcctaa cctttgtttc atacccatag
    17761 aaaattgtga agctctcttt ttacctgtat ttttcaaatt tactatttcc aacacttcat
    17821 aaattttatc tttaggtatt cccaatattt ttgtatgtac taacaaattt tcctctgctg
    17881 taaggtttcc atacaatgca ggggattcta ttaaagaacc tatttttgca agagattctc
    17941 gtttccaagg ctttctatca aaaataatac gaccatttgt tgggcgaaga agaccaacaa
    18001 gcattttaag tgttgtggac tttcctgctc catttggtcc taaaagtcca tatatactat
    18061 ttcgttcaat ttgtagagaa acattgttaa ctgccatttg ttctccatat ctttttgata
    18121 agttttttgt ttcaagaatt aaatctttca tattaatttc tcccttcttg taatacctta
    18181 aatctgttac taagagaata ctaacatgaa aaaataacaa ttctataaca cctactaatt
    18241 ttaataaaat ttaaaataaa aaatagagtt accaatttta ttttggcaac tctatttaag
    18301 tttatgtttt acagttatat tagatttaag cttagtttca ttatcacact aacttcaaat
    18361 ttactctata atccttaaat caaacactaa tttattaatc aaacactaat ctattaattt
    18421 atatttcttt agacatctaa tatagtttgc tactaaataa taattattta tatcatattt
    18481 atctaatcct ttaaaagaag ttatttcatc attaagctca tcactacctt ctacaaaaac
    18541 ataacctgtt tcaaattcca tacctacaat aatcttacag ccactataaa tttcctcttg
    18601 agtatcgtac aatttttttg catttccttc atttggaatt gtaaatacaa taggttttaa
    18661 ttctttatca ctggaaatct tttgtgaaaa aggcttgttt aacttaatat tattgataac
    18721 tacatgttta aattcctctg aagtttcttc tactattaca tattttcgct ttaaaaactc
    18781 tattatttct aaaacagact tagaatttac ttttaaaata cttttatatt tttcatataa
    18841 agatttccac tgttcaattt tttcctgtgg tacttcttgg atatgtgtac ttattttttc
    18901 ttgagatatt tttttcaatt aaactctcct ttcaatactt aaatatcctt ataaatctca
    18961 catgactaaa gttacaaggt tcctacttaa tagaattttg catacttaaa tatgtcttat
    19021 taattctcta caggatatct ccgtagttcc tacgattctt acataagtta cttattttaa
    19081 attgttatta gttagattat aaaaattatt tattttatca ctaatataat actcttaaaa
    19141 actttacaaa tcaatttaaa tttgctacct tttatttata acaacttata gtacatttct
    19201 cctatgcttt tcaatatatt acttttaaaa ggaggttttc attatgattt taaatagagc
    19261 tgagcttttc cgaggatatt ctttaaatag atttaacgag atatgcaata tattatcagt
    19321 aaataaaatt aaatataagt ataaagtgaa aaataatgct tactctaaaa aacctttttt
    19381 taatgaaata acacttggca atttaggcca aaaggaagat ttctcttatg agtacttaat
    19441 ttatgttcat aaagacgatt atgaacaggc agagtctcta ataaattcta agttgcgata
    19501 gattatctat aaaacatgac attctgttgt catgttttat atgttattat actttcaaat
    19561 atgctactat attttcaaaa ttgtgtgctt taaaactagc tttttttagt aatttaaagc
    19621 ttaaaactaa ggagactcgt tatatgaaaa taaatataaa atcaaagtta agtgaattta
    19681 ttaaacagaa caatcttttt gatgacagca gaataatatc ctatcttcct aatataacaa
    19741 tagaaacaga caaaataaaa actataatga ttaatgaggt tgtcccaact aacaccaatg
    19801 acgattttta tagtgtagat aaagatgctg attatttaaa aactactatt cctttatttg
    19861 acagtgcagg aataaaagta tctaacataa atgatatcct agatatgggt atctatataa
    19921 ctaatgcagt aaaacttcct aaatcagaat atacaataac tcgtgataca attaaattac
    19981 atatgcctat tttagaagaa gaaattaaat tatttaaaaa cctagaggta gtaatgttaa
    20041 tgggagatgt tgcaaaaaaa tcttttaata tgattactaa aaagcatata aaaaagaatg
    20101 tcatcccttc catttcaaca tataaacttc gtaataatga actttattat gataatttaa
    20161 gagtatttcc atcatatatt atgactggtg gaaacatact gattgaaaaa tcaaaatttg
    20221 aaatggcatc agaagatata aaaaaaatgt tcgagattat aaacggttga tattacaaaa
    20281 taataaaaac taatataaaa taaaaagtgc ctcaaaaact tctatttatg aggcactttt
    20341 attttatatt agtgtaacta tatactatta acataattta ttatttatat tgttcctttt
    20401 aatctctatt ccatttagtc taggtaacat tactctttac aagattactt tatatttctt
    20461 ttatatctct aacgttttta tttaataaaa caattattaa tacaagtcaa atatatctat
    20521 ttatctaaaa atatatcttt tacagtaata tctggatggt gatacttctt cctattcgtt
    20581 aaaaaagatt tttttacatt aatagcctct tttggacacc agtgaaaaca agctaggcat
    20641 ctttcacact cattacccca ctttggcttt ttatcaacta tacttatatt ctctacagga
    20701 caaacccttt tacatgtccc acatctatta catttatttt ctgcaaaaaa tttagtagtt
    20761 gcaatttgca tattcttacc tactaaacga agtacaacac tatctatatg tgactttaaa
    20821 ttgttttttc cttctatatt tccaatctcc aagttattga tacattttgc aatctcagct
    20881 accctattag tctgattttt caatctttct tcttcaacat ctggaggtgt tatcaaagca
    20941 tttccaggca tattgattaa aaaaccagca gataattttt tccccctttt tttaagtaac
    21001 ttatctatag taaataaact atgtccagct accccattac aagtggcaat ggcatatata
    21061 tgagtattaa tattaaaatt taatttctct acaaaagact ttactatatc aggaacatca
    21121 gcaaaataaa caggaaaaac aattaccaat atatcttcat ttatgtctat attattttct
    21181 tttctatgta tagctaaggg tataacttct ttacttccat ttaacgtttc ttttaaaata
    21241 tttgaaacta ctagcgaatt tcctgttcca gaaaaataat aaatcttact attcattctt
    21301 atccctcttt ctatatttct acatcatcaa tagttaaaaa gtctcttaaa ataggattta
    21361 tatttacttt ccaattctat ccaaaatatc agtaaacaaa ggcaattctt catcagagat
    21421 agatttaaat gtctctgttg catatatact tgcaatttca tttaatggtt cttctaattc
    21481 tctacctaaa tctgttagga atatttgaaa cttacgtcta tctttaacat ctactttacg
    21541 gacaattaat cccttatttt tcataacatc taagattctt gtcgtattag atttatcctt
    21601 acatgatttt tcacataaat ctatctgaga aattccatct tgctcccata gaaaccttaa
    21661 taacgcataa tggtcagttg ttatatcata tccattttgt ttaaacatct tttttaaatc
    21721 atcttttact tttaacgaag tacttactaa cttagttgta gcttcatata gtttctcaaa
    21781 atctttcatt ttacctccat ttaaataatc ccttgctcct cccatctatc aattattccc
    21841 ttatattgct caatatcttt aaaagccatt ggctttaaaa cagcatttat agcaataaaa
    21901 ttatagattt gagaaagcac aggtggattt ttcttttcct ctatgcaata aataaatttt
    21961 ttagatacct tttgtatctt tgattctact ttttcagtta taggattttg attaaatcca
    22021 gtttttatag ataaactacc acaaacataa cctcccgaat ctaagaccat ggattttata
    22081 aaagaaattg cttttgaacc acgtgcattt tcatatgtag taactgctat acatggctta
    22141 cgatacaata atctttcaag catcatatgt gctctatctg taaacacctt gaaaagtcca
    22201 gttacactac ttccataagt tgggctacca aatataacac catcactctt agtaattata
    22261 tcatgaatat actctacttt atcattttga ttgatacact tgcccatttt ataacaattt
    22321 aaacaaccaa tacaatatga tggattgacc tcacttaaat caaaataatt aatttttaca
    22381 tctggtatta atctttctat atctttatac aagtaagtca aaacttttga tgtagctcca
    22441 ttttttctag ggcttccatt tataactgta attatcattt ctatatctcc tttttagttg
    22501 ttatatcaac aataagtata tcaactatat ttttagctgt caatataaac catttattta
    22561 aagctatact tacacagctt aaataaaagt tttatttaac tacatgtgct tatttaatca
    22621 actataaatg aattattttt aataattgac aaactaaatt aatgataata tactttatgt
    22681 attacaaatg taagtagaag gaggtatagt atgaaaaagt taccacatat ttcagaagca
    22741 gaatatcaag ttatgaaaat tatttggaaa tatgctccta tcagcactac tgaagtaatt
    22801 gaaaagttgg tagaaacaag tacatggagt cctaaaacaa ttcaaacaat gttattgaga
    22861 ttagttaaaa aaggagcttt aacatatgaa aaaaatagca gagtattcgt ttatactcca
    22921 cttgttaaag aagaagaata cgtagctaca gaaagtagtt cttttttaaa tcgtttttat
    22981 aatggtactc ttaattctat ggtactaaac tttttagaaa atgataaact atctgaagat
    23041 gatattgagg aactgagaga aatcttaaat aaaaggacga ctaaggggga taaataaata
    23101 tgtatgcccc attctttata cgatttttac tcagtacatt aaccttatca atattaattg
    23161 ttgtaatttt acttgccaaa aaagtattta aaaaacatat ttctgcaaag taccaataca
    23221 atattggatt tatattgctt ataatgttaa taattccatt tgtccctctt aaatatatta
    23281 atcttggaaa tgtatttaac tatttatata cttttaatca acttggaagc agtactaata
    23341 ggacttctat gataaaaaat agttcaaatt ctgtaattaa taactcaaat gcattaaacg
    23401 atttttcagt atccctaaat caagttaatt ttgatagttt aaatatttta ttactaatat
    23461 catggataat tggaatttta atcatgatag ctattactat tcattgcaac attaaaataa
    23521 aaaatatgaa aaaatcaata caaattttaa aagatgagga gatagtattt atatttgaaa
    23581 agtgtaaaaa agatattggt ataaataagg attttatact atgcaaatca cctattataa
    23641 atacacctat tacatttgga tactttaaac cacacattat attaccagat aagtctatat
    23701 ctaaattatc tttaaaagat ataaaatata tcttacttca tgaattacaa cattttaaaa
    23761 ataaagatat actgataaat tatataatgt gctttcttca aatattatat tggtttaatc
    23821 ctcttgtatg gtatgcattt aaagaaatgc gtatagaccg tgaaattgca tgtgatattt
    23881 cagttttaaa aaagcttaat aaagatagtc atatagaata tggacaaact ctaataaact
    23941 ttgtggataa tatttcaaga ccatcaaatc ttacacttac atcagatatt ggtggttcaa
    24001 aaaaacaaat aaaaaagcga ctattgaaga taatggattt tcataatgaa tcaaatacgt
    24061 taaaattcaa aagcatttta atttttacat tgatagcttt aattgtattt ggaaatattc
    24121 ccaaactatc tattatggca gatatcaacg aaaaatatag acctacaaat gaacaaactg
    24181 tgtacgagga cttaagtcag tattttaaag gttttgatgg aagttttgtt atgtatgatt
    24241 taaaatccaa gcaatatcat atttacaata aagaaaaaag taaaacaaga atttctccaa
    24301 attctactta taagatttat agtgccttat tgggattgga aaatgggata ataacaagaa
    24361 ataattcaca tatcccttgg gatggaaagc atcattctaa tagttcttgg aatgaagacc
    24421 aagacttatt ttctgctatg gaaaactccg taaactggta ctttcagact ctagataaaa
    24481 aaacagggct aaaaaacata caaacttatc ttaggcaaat tggatatggc aattatgatg
    24541 tttcaggtgg tatctcaaac ttttggctag aatcttcatt aaaaatatca cctattgaac
    24601 aggtagaatt acttgaaagt ttttatacaa ataagtttaa atttgaagat aaaaatatag
    24661 aaacagtaaa agattcattg ttaatatcaa aaaaggatgg ggtatcactt tttggtaaaa
    24721 caggcactgc caatataaat ggaaaagatg taaatggttg gtttataggt tatgtggaaa
    24781 aaaatgaaaa tacttatttc tttgctacaa atattcaaag caaagattat tctaatggta
    24841 gttcagcatc taagataaca ttatccatat taaatgacaa aaaaatctat aatccttaat
    24901 atatttactg tacattctaa aaaattcaca taattttcat attaattatc aattaaattc
    24961 atatacaagg cttattatat aaacatagaa gatagtaaaa ctagcttaaa aacataatat
    25021 aataccctat acaaatagtg gcaactctcc ctcacatcct caattgttgc cactatttaa
    25081 ttttttgtat gaagttaaat atattttcat catttcaaca atttgggtgc aatacaatct
    25141 ctattttgaa acaacctctt taattgtttt agagtttatg aattatttct catactttaa
    25201 aaagattgaa ttactcttga taatcttctt ttgtagtcat tacatatcac ctactactca
    25261 acacatattc tttgacctat ttttacatcc ttgtttatat ccaaaatatt taccttctta
    25321 ccatctggaa acaataccat tgtcaaagta gactttccta attcatgtat tctctttaaa
    25381 tcaacattaa caagaacgtc tccctgttta accatatctc cttgtttaac agcaacatca
    25441 aagccatctc catttaatgc aactgtatca attccaacat gaattaatac ttctccatta
    25501 gttcctttaa tcccaaatgc atgtccactt gaaaccactg tttctaattt acctgaaata
    25561 ggagaaacaa tttttccatc tataatatca atagcaaaac catcacctaa cattttttta
    25621 gaaaatgttt catctggtac attttctaat gcaacaattt tccccttagc tggagacaca
    25681 aagttttcat tattttcatt aaacactaat tcttcgtctt catcttcatt tatagcttct
    25741 ttaggcactc caaataaaca tgttacaata aagcagaatg caaccacaaa tattgatact
    25801 aataagtatg tgattaaatt atatccatca tagatataca ttaaataaga aggtaaaact
    25861 gctaatccat atgaattaga ttgaatatgt aaaattgata atattgctgc tcctaaacct
    25921 gaagatgcac acataataat caaaggcttt aaattatatc gaagctgaat cccaaataaa
    25981 acaggctcac ttataccaaa aagttgagat agcattgctc caaccgcagt ttgtttaact
    26041 tgattatttt tagatttttt cataaatgct agacatgttc caatattcgc aaatccatac
    26101 attgcacaaa gagtaataag ggcattaaat cctgtatttg ctaatagtga tgtttcaatc
    26161 attacataag tatgatgtaa gccagtaata acagctaatg gatatgtaaa tccaataata
    26221 aatcctccaa ttcctaaagg caaatcaatc aataagctga tgatatcaac catttttaat
    26281 tctattgtat gcattatagg gcctacacct aacaaaataa ctaacattgt tattagcata
    26341 acaaaaaatg gtgtgaaaat aaggtctaat acattaggca tatgtttacg gaaccatttt
    26401 tcaagattag ctccaataaa tgctgtaaca atagctgtta aaacacttcc ttgacaacct
    26461 acaataggaa taaatccaaa tgccataatt gcttcaattt catttgatgg gtcagcaaca
    26521 gaatatgcat taggaagtat tggggaaact aacataagtc caataacaag tccaataaca
    26581 ggtgttcctc caaatacttt aaatgctgac catacaataa ttgctggtaa aaaagcaaat
    26641 gctgtttctg taagaaccga aacaagtgtc tgaatataca atggaatatt tgcagaagac
    26701 attccaaata atccaagtac attatcatta aacaaacaac cctttaatcc caagaataat
    26761 cctgttgctg caataactgg aataatagga acaaatatat ctgctagagt tctcataact
    26821 ttcttaaatc cagtttcatt atttttaact aattcatctt gttctttttt tgataaagtt
    26881 tttaatccca ttttttcaac ttctgcataa actttattaa ctattcctgt tcctaaaata
    26941 atttgatact gtccagaagt aaagaaaacc cctttaacct catcaacttt ttcaactttc
    27001 ttatcattaa taatttctct atctttaacc atcactctta atcttgtggc acaatgtgta
    27061 atagaaataa tattatcttc accaataatt ttaataagct catttgctat tttattatat
    27121 ttattcattt ttctacctca ctttcaataa tactgcatga aatggtttca aacgcactgt
    27181 tttatttaat agatttatat catctaaatt ttggaataca atatcatatt tttcttctac
    27241 tcttatttct acaggtttat cagaaaaatt ataataacaa tcaataacta ccaaatctaa
    27301 ttgccttcta tacgcaataa tatcatcact ttctatatca ataggttcta ttgaaccata
    27361 aatcaaacaa tcagaatact cactatcttg tctcaacttt atcatctttt tataaaattc
    27421 taaaatactc tctgaattgt taatctgact ctgtgcatta atatctttat aattttcaat
    27481 aacctttaac catggtgttg tatctgaaaa acctgcattt tctttatctg cccatggaaa
    27541 tggcgttctt gcattatctc ggcttcttaa attcacaaat tctaacgctt cttcttctga
    27601 gtaactttca tctattgaac gatagtattg gtcaatgcta gataaatcat taaaatcatt
    27661 tatggtatct cttttaaaat ttgtcattcc taattcctgt ccctgataaa taaatggggt
    27721 tcctcttaaa aagaaataca ttgctcccaa catttttact gcatcatcat tcctttcgta
    27781 ttgcaaaaga tactttgttg tagctcttgg ttggtcatga ttttcaataa aattagctgc
    27841 ccatccatgt tcctgaacat acatttgact atctaaaatg cattttctta aatctggaat
    27901 tgtccatgaa attcttttaa accactcact accagaagca acatctaagt cagcatgttt
    27961 aaagtcaaag accattgaaa agaaaccatc ttctccaata aaatcaccta gctggtcata
    28021 gctaatacct gctgcttcag caacagtcat acaatcatat tttttaaacg tattttcttt
    28081 taattcataa agcatttttt ctattccagg ctgatttcta actgtttttg tacatttaac
    28141 aagaccatca acaccatcac tttctaaact ttgaaaactt aaattctttt tgatgaatgt
    28201 aatagcatca actctaaatc ctgccactcc catttgagac caggtattaa tcatttggta
    28261 aagttcactt cttaatattg gattttccca attaaggtca ggttgttttt taccaaaaga
    28321 atgaaaataa tattcgtctc tattctctac cttttcccaa acacttccac caaaaacaga
    28381 cctccagtta tttggtaaat cgtttccttt tttaaaaata taataatcat gataaggact
    28441 ttctggattt tttaatgctt cttgaaacca aggatgttca tcacttgtat gattaagaac
    28501 taaatctaat ataattttga ttcctctatc tttactttct ttaattaact cttttaaatc
    28561 ttccatactt ccaaattcac ttgcaatatc caaataatca ctaatatcat acccattatc
    28621 gtccataggt gatttaaaaa ttggacataa ccacaatgat gtaataccta aattttttaa
    28681 ataatctaaa cgagaaataa ttccctgtaa atctccaatt ccatcattat tactatcttg
    28741 aaagcttctt ggataaattt gataaacgat ttctttttgc caccatttct tttccataaa
    28801 ttactccttt ttatatgata tttcttcttt ttaattcttt ataaattcca ttatcgaata
    28861 tgtcctcaca tatagatgtc gctatatctt taagttgttg atgactattt ttcattgcaa
    28921 ttgtaacgtc tgatgcttga aacatgacaa catcgttttg accatcacca aaacatatgg
    28981 tttctttttg agttactcct aatctttctt gtaatctctt aattgctttg cccttatgaa
    29041 aatctttttg aataatttca taatactgac tagaaatatc tctctgtgct aattccattt
    29101 tatcttgtag tatatttttc acttcgtcaa ataccttttc atttgaccat aaacaaattt
    29161 tatgtatatc ttgtgactta tactcttcaa tgttattttc atatgttatt ttttcttgga
    29221 tatgttgttt atttatacaa gaattagttc ctttcaactg attcattgtt tcaaatattt
    29281 ctttggcttt ttgattcatg aaaacttttt cctgactctc aatcgaaaaa gcaacttctc
    29341 tcttttttaa taaacaaaca acttccttta tcaatctttg attaaaagat tgattataaa
    29401 gtaattcacc atgatattga atgtaattac ctcctcctgc aatatatcca tcaactccta
    29461 atgataaaac atcatcttgt attgtaccca tacttcttcc agtacaaata acaacactgc
    29521 aatgattctt ttgacataaa cgtatagcat gctttgcaga ttctgggatt ccatatacct
    29581 catctctaag cgtaccatct atatcaagta ttaataattt gtacaatttc atcttccttt
    29641 cttactatca aaatcataat agcattcaaa aaaaaagata tcaatatttt gatatctttt
    29701 aaaaaatatg ttcattttta gcacaaattt tcattttcgg ggaatatgtt cactttctaa
    29761 atacatcttg tatttaatat aaagcagttc aattaaaata aaataaggtg ttgttacatc
    29821 gtagtccagt tgtttatcta cagaaataga tatagattga atatacaggt tttcatcaca
    29881 taatcttgct aaaggattac ttatcatttt tgtaatagaa accatataaa ttcctttcat
    29941 tctcatttct ttagcaaaat caataattgt agatgcatct ccattcaatg aaataaagaa
    30001 aatcatatca tctgttttta taaaatgatt aagtgcctga atcatatcat aaccataaac
    30061 atcatatata attttccctg ttggtaagaa aatccttttc atctctttag caactctttc
    30121 ctgagaataa ccttctccaa agacgattat acgatttgcc tttttcattc tttcaaacat
    30181 aatcgaacaa tccttcttct ctatatcatc tatcacctta ttataacaat ccattaattg
    30241 gtgtttactt tgatttttat attctacttc tgtttcattt aagcgtataa taactcttaa
    30301 ttcactatat ccaggtaatt gtagtttttg agcaaaacga gaaagtgcac ttgtagatat
    30361 atggtatttg tctgcaaatt catctataga aagtttaata caatcttctt tatgattaag
    30421 aatacactca cagatatact tatcatttgc tgtaaatgaa ttataatatt tattgaaaag
    30481 tccttcaatt ttcattcttt tctccttcta tactctatgt aattcatatg aagcaaatct
    30541 aatagagcat aaaatgctcc tacgacttca taagatatag cttgtaatgt atcaagtttt
    30601 tgcgtttcaa catacagatt atactcacaa agtgttgcaa ttgtattatt atcaagccta
    30661 gttaaagaaa tcgtatgtat atgattgttg ataaattttg caatttcaat tcctgctttt
    30721 gtttctccag acaaagaaat gataataaat aaatcatttt tttgaaatga ttctctcata
    30781 aattcaattt cacctatatc aaataagtca ataacacatt tacctactga aagaaatatt
    30841 ctctttattt cttgagctaa acttttctgt tcattacctg tcccataaat ataaattgtt
    30901 tctgcatcat atattatttt acaaatatct tgaaatgata ttttttgtaa ttcattcatt
    30961 aatagtcgat aattctcgca aacatcatga aaattattat cattattaac aaattctagt
    31021 tgattatttt taagtaaata ctttaaatca gagaatgaat ctaatccaat ttttctacaa
    31081 agccttaata aagtagctct agaaacatta cattcatttg caattttttc acacgaaaaa
    31141 ttacggtatt ctctttgaca aatcaatatt tgtttaataa taattttatc attttcattt
    31201 aaattatcat aattttggtt aactaacgat tctaatctca ttttttccac tccctaaata
    31261 ttattttaat atacattagt tgctttttct agggaagagt tttaaataat aaggataaaa
    31321 taatcatttg attttatttt tattaaaata aaaataacct gctaaaagca ggcaccaaaa
    31381 agtacttatc ctttcgaaca tgtactttag atattgcttt cttcggaagc ttcatctctt
    31441 catactctaa aatatagttt tttctaactc aaaagtattt tccatggtag aaattacttt
    31501 aattcatctg ccatttactt tttatcttat ttaataatta ctattacttg aatatttact
    31561 tctttaagta aatttcatag ttaacaaagc taaaactatt attatagatg ttaaaataca
    31621 aaattgtaag tattgttata aatatgctta cagctacaaa tatacatgat atattaaaaa
    31681 gaagctgtaa attattatca tacatacaag caactatgcc tctattaatc catgttaatt
    31741 tttattattt aatacaagga gttccatgag acatacatat ccattcatat tttaaattat
    31801 ccaattctct tatagatttc accatctttt tataatccca attccatgga ttaggataag
    31861 gaactatatt tccattctta tttttgacta aatcacctgc aaataataca gcttcaaaaa
    31921 tcatgcagac atgccctgga gtatgaccag gagtatggat aatttttata ttcccaatct
    31981 gcatattctc tccataaggt tcaacatcct taatgagcct tctggaaatt gcctttccaa
    32041 taaacttctt aaacccaggt ctatccttct ctcctataat aaaaggtata tcctctacat
    32101 gagcccatac ttttgctcct gttttttctt tcaataattt aatattagat atatggtcaa
    32161 catcatgatg agttaataaa atatgcttaa tatctgtaag tttaatgcca agtgttttta
    32221 attctttaat tatatttttc cccataaaag gaagacctgt gtctataaga ataatttctt
    32281 tatcaaacac aataaaacaa tttgagccac gagtactatc taacattaat acattttctt
    32341 ttatcttcat aaataaattc cccttatttg gtattttacc tatataatta attaataaag
    32401 ttataaatag ttataatact tgatattaat tttatttttt attaaagtat actataggta
    32461 tacaagtatt atatttacat aagtatacca tcagtatact tttttatcaa tagaaaggaa
    32521 taatttatat gtcagaatta tcaaaaagag ataaagaaaa aatacaaaga gaaaatgaga
    32581 ttattgataa agcagaaaaa ttattttgtc ttaatggatt tgataataca actatgaatg
    32641 aattagcaaa agaagttgaa tatactaaac gcaccatata caagtacttt tcatgtaaag
    32701 aagatttatt ctttgctgtt gtcttaagag gatataaaag actttgggat aatgttaaaa
    32761 tagaaagtgc aaaaggcaaa acagggtttg aaaaaataaa actttcatac tttgcttttc
    32821 ataaatttta ctctgtagaa ccttctctat tatgccttat gggaatgatt ggtattgtaa
    32881 actctgaaaa atcggatact gatatgctgt ttaaagaaaa gtttttttct tttaataaat
    32941 ttatgtttga tgaaatacaa ggaatgtttg aaattggaaa aaatgataga agtattaggc
    33001 atgatattga aatacctact cttatgtatt catcaatatt tacacttaca ggatttttta
    33061 atttactatc tgtaacagga aaatcatacc tgaacaactt taatatagat gaggaaaaat
    33121 ttattgaaac aactcttgct cttttaatag attcaattaa agcataaacc taagtttata
    33181 aattaaagtt tatatactta aatgataatt ttttctatct ctaatataaa aaatattaaa
    33241 cataaaacta ataacttagt taatggaata actaaattac tgaaaggatt aaaagtaaat
    33301 ggctaaaata cttcttttag aagatgatga tacaattaca tttggaataa aaacttccct
    33361 tgaaaagaaa aatcacactg tatgttgtta taatacgatt gctgaagcta aaaaaaattt
    33421 tgataaaact attgatttaa ttctgcttga tttaaatcta cctgatggta ttggatacga
    33481 gttttgccaa tatgttaaaa gtatagaaga tactccagtg atatttttga cagtccgtga
    33541 tgatgaaaaa gacattatac gtggattaga tatgggtgca gatgactatg tggttaaacc
    33601 gtttttgctc tcagtacttc agtccagaat taatgcagtt cttagaagaa ctcattcaaa
    33661 tactaacaat actattacat gtgaaaatat aagcttgaat aaggttgaag tcaaagcata
    33721 tttagataat gttgaagtac ctttgacagc aggagaatat aaacttcttc taagtcttat
    33781 ggaaaataaa aatagagcta tgactcgtac aaagttacta gaaatgctct gggatgtaga
    33841 tggtaatttc gtaaatgata acactcttac tgttacaata aaacgtctta gagaaaaatt
    33901 aaaaaatcca ccttgtataa aaacgttaag aggcatagga tatagaatgg aggacagcta
    33961 gtgaaaactc caagaaatat caacagtaca gttgttttta taatattctg cttaattgta
    34021 acttttttat tgagtgtaat tctaacaact ataacttctt ttaatgaata caatgtactg
    34081 tctaaggctc ttggtgcatc atatgagtac aatcctaagt ctacagaaag tatactaaaa
    34141 tcactgaaaa attctagtga taaggatttt aaaacaggaa atgaaatttt atcaaaacat
    34201 ggatattcta aaagtacatt ttggagtaaa aacttttttt attttctagt gatatcgttt
    34261 ggtataattt caacaatggc tacagttata tatataatca aacatagaac aaataagcaa
    34321 aaactgagta gaataaacaa tcttactcaa tacttagaag aagtgaattt aggaaaagag
    34381 tctgtacttt tacgatgtga agatgaattt tctcaccttg aagatgagat atataaaaca
    34441 gttggggaac ttcgttgttc aaaagaattt gctttaaaag aaagacaaac tctttcagat
    34501 aatcttgcag atattgctca tcaacttaag actccaatta cttctatgtc acttatggca
    34561 caattacttt cagaaaattg tccagatgaa gaaatagatt atattaacag acttacaaat
    34621 caaatctcaa gacttgagag actagtttca tctttactta cattatcaaa attggatgca
    34681 agtactttaa tatttgaaag caaacttata gatgtacatt ctcttcttac ttatgcaatt
    34741 gagcctatag aaggttcttt aagagaaaaa catcagacat ttactatcga ggcttctcct
    34801 tcggtagaat ttgaaatcga tattaattgg actttagaag ctcttttaaa tattcttaaa
    34861 aattgtagtg agcatataga agatggtgga tatatagttg cttattatag tcaaaatcct
    34921 ctatatgttg agattatcat agaagataat ggtaaggggt ttgctcagga agaacttcca
    34981 tatattttta atagatttta tcgtggtaaa aatgctagta aggacagtat tggtataggg
    35041 cttgctctat caaagtctat tatagaacgt caaaatggta ctctacatgc agaaaattgc
    35101 cataatggtg gtgcaagatt cataattaaa ttttacacat aaaatgctgg attataggta
    35161 aaatacctat aatccagcta tatctatatt ctatgttatc tattgtttta gtaatatact
    35221 tctatttaca tttaaggttt tattaaacaa acaacttaac tatccctata cttgtaagta
    35281 gtcttaaatc gaaaccttta aataagttca aatagatttt gtttatattg tttcattttt
    35341 tatagattct actatatttt cattcttcaa ctgcttgtat cctgtcatat atgatgctat
    35401 tacaataaaa agtatcaata tagtaaaacc tattattgta gatataggtg caaatggtaa
    35461 atactctata aaatatattt catttatacg tagaaaagca tacactattc ctatttgcac
    35521 aggaatacta agtagtaatg gtgtaattcc taagaataaa ccttccaaaa taagcatctt
    35581 ttttatgcct tctggagata atccaacaga acgcaacata gcaaactctt gtcgtctact
    35641 tctaatattt cctgacactg tagcaagtac atttgataat ccaatcatag ccaataaacc
    35701 tgtaataaag cctactatta aattcatggt ctttattcca ctagcctttt gtttttcact
    35761 ttctaaaaca tcctcaatac tataatctcc tgaaccatag tctttattat aaatggactc
    35821 taatttttct cttactctag gaatgtcctc acgattattt actttaaata ctcctatcag
    35881 cctatttgaa cgtaacctac ttacttcatc ataattttta gatatttcct gtaccataga
    35941 cattggaact atttgcatta atgtatactt tgaactagta tctactattt taggaagtgt
    36001 gcttgtaata gctcccactt tcatgttgta agtataatcc cctttatctt cgtcatatac
    36061 tttttcattt aatttaatgt tatctcctat tgaaagattt aaataatcta tatatacttt
    36121 atttctacgt gtactacgat taatatcttc ctccttatta tatacaattg aggttggatt
    36181 ttttgtatta taaaataact ttgggtctac agaaatttgc ctacaatatt ctgtaaaact
    36241 cttatcatca agcccaatta tatttgaaat tatacgaaac ttgccatcac gttctatagg
    36301 agagtatttt ttagatgaaa ctatactttt aaatcctcca tttttcgcaa gttcttttga
    36361 tgcatctttt tcagtcagcc aagtggaagc agacattttt tgataatata gtgaactttt
    36421 tacaccttct gtattatcta atttttcaat gaaagcagac tctataggtt cactattttc
    36481 caaataaact gctatatctc tttggtcctt ataattatca cttccataaa cagcttttgc
    36541 tccttcttga tttgcaatta tatgcaaaaa acatgtaagt aataaaaaag ataatgttaa
    36601 agatattgtt gcagtacgat aagatttttt acgagcacgt agtgcgtttg atgcaagttc
    36661 tccttctatt ccaaatatct ttcctaatga atatttatgc gactttttga ttttaacact
    36721 atctccttgt cgtattgctt ctataggaga tatctttgat acttttcgtg ctggtataag
    36781 tgcagaaatc cagactgtta ctattgtaag taaaactgct ggcagaaaag caggaatacc
    36841 ataagtaaat accacttctg gaacatcagt atgataatta actgaattaa cataaactat
    36901 aagcctatta cacaataacc atcctaaaaa taaacctatt ggtaatggta taattgtcag
    36961 taaaaagcct tcaaacacta cagacctttt aatttgtttt ggtgatgcac caacactaga
    37021 taatatccct aactgagtaa gtctagcatt tgcagacagt gaaaacgcgt tgtaaatcac
    37081 cattacaaac acggctactg taaaaattgc tattgtaaga tacataatag gagttgaaaa
    37141 ttttgatata ctactcatag atgctttctg ttctggtgaa aagactaaca tctttaaaag
    37201 ataattacta ttgtatttaa gattgtattt tccatattca ttagtcttcc atcccaatga
    37261 tttagcaagt tttggaagtt ccttatacgt gtctcttata tttttaaatc gcaagtacac
    37321 agttatacta tcattaggtt ttatacttgt tttatcaaga tatccaagag ctgtatatgc
    37381 aggtattact gatgaagtag aaacatctac ctttcctact attgtcaatg tagcctctga
    37441 aattttttta aaactttccc ctttcgattt tggggctatg ggttcaagag gctttccatt
    37501 taagattcta tatcctattg ggagagtaat tttatcacca atttttacac ttggattatt
    37561 ctcaaaatat tgttttgata atgcaatttc attagcatta tttggtcgac gaccctctat
    37621 tatagtatcc ttttctggca tagaattcca atactctgat gtagcatttc tataaattat
    37681 ataatctctt ttctcatcat taattttgcc tacataccat tgtcctttta ccattacatc
    37741 atcaactgaa gaaaaatttt caattaatgg taagtcactt ccataagtat agtcaaacaa
    37801 ttcaccatgc cagttacctt ctttctcaat tgaaagtctt ataaaatctg tccacatggt
    37861 atacaacagt ccacacaaag aagacatcat ggttgtagta agtaatatag ctaacattat
    37921 agctatacta ctacgtttat tgcgtcgtac aaagtctaag gtgtattctt gaagaatatt
    37981 catttcttca ccaccttttc agagacaatc actccatctt ccatagttac aatacgattt
    38041 gcctctagtg ctattttctc atcatgagta ataagaagta ttgtctgatt aaatcgttta
    38101 tttgataagt ttaacaagtc tacaatttct tctgaatttt ttctatcaag attaccagtt
    38161 ggctcatctg ctaataaaat agctggtcta tagattaaac ttctagctat agataccctt
    38221 tgttgttgtc cacctgaaag ttgacttgga agatggttta atctatcaga taatcccaat
    38281 atagatacaa tctcagagaa tctatcctca tccacctttc ttttatcaag taacatgggt
    38341 aataatatat tttttctaac atcaagggta ggtataagat tataaaattg ataaataaga
    38401 cctacttttc tacgtctaaa aactgcaaga gcttcttcct ttaaacttga tatttctgta
    38461 tcttcaacat aaatctttcc agatgtaggt ctatctactc ccccaagcag atgtaacaat
    38521 gtagatttgc cagaaccaga tgctcctaca atggatacaa aatcaccttt ttgaactgat
    38581 aaatttacat tattaagggc tgtcactctg gtttgatttg aaccatatat ttttgtgaga
    38641 ttttcacatt ttaatatttc catatacaat catctccttt atggttaaat tttagcaacc
    38701 taaaatgaca ttatagtgac atgaatacaa atataaatta ctattaaaaa tattgtttag
    38761 tttttgcaca ttatttaaaa ttataatata aaacaaaaac tatgcaatat attttattta
    38821 tacttttaat atattacata gtctttaaaa ggtattaact ttaaatctat aggtattcaa
    38881 tttaataatc aatagcaagt attttatgta ctaaaggagt tttgctctat atagactcaa
    38941 tttagtaaaa ttatttaata aatattacag actataggta aataatattt acaaataaaa
    39001 ttaagtttat tcccttacac tataatattt cttaattata ttaattgcaa tttcctttac
    39061 ctttggccct gtaatttctt tattggaatc atctgattta atagcaaaat aataggtgtc
    39121 accttcacgc tctagcatac caacaaacca tgcatccaca cgtttatttt tttcatcaaa
    39181 tcctgttcca gtttttccat atacatttat gtttttatca tttatatcta ttttcataat
    39241 atctcttaaa atattaatat gctctttttt aaagtttgta tctccctcaa atatttttgc
    39301 cattgtttgc acttgttctt ttggagatat ttgtaatgaa gattctaacc aaaatccatt
    39361 taaatgtccc tttccatttt ttaaatcgcc ttcccattcg ctaatatcgc aattcccata
    39421 ctttaaatca tctaaaatgt tttggacaga tttagcatca actttatcta tcaatttttt
    39481 gtaataccaa acacaagact ccttaaatgc ctcttctaaa ctaagatttt tattccaatt
    39541 tttatttgga tactctgtac catcatatcc cataactgat tcttttgagt ttattacccc
    39601 tttttctaaa ccaattaaag ttgaaactat tttaaaagta gaacatggtg accttcttgt
    39661 ttcaattaat tctttgttat atatattata ttctttattt ttagtattat aaaaaatagc
    39721 tcctccacta ataccttcaa aacaatcact atagtcaacc atattaattt tttctttttt
    39781 gtttttatca ttgttttcaa ttttggattt atttaattca gacttattta attcagattt
    39841 tatataattc ttatcagttt tttgatttat atcgttatgt tttttcccta cataatacat
    39901 taccataact acaccaacca ataagatagc tatccaaata aaattctttt ttcttttcat
    39961 aatttatatc atctcccata tttaaattta taacctacta ttaaaatagt agacattatt
    40021 tgtaatatac tccaatctag gttctactta taagaaacaa gactaaatga atagctgtat
    40081 ctcttatatt ttcttcacct agtacaatta tgttgattaa acctattgcg aagataatta
    40141 ggtaactatt aataacatcc tgtcctcctt ttaatttgtg atataataat tgattccaca
    40201 tttaaaactt actattgtaa tatttaaata ttacaatagt aagttttaaa tgtcaagtta
    40261 gtttaggaaa gttattttaa ttgtataatt gttattacat ttataaaata tggcatgttt
    40321 aaaattattt tgctattggg aggattaaga taagcagtat tataaaagct ataaattata
    40381 aaatttaata tataatttat agcttttact tttacaaata ttgtttttat tgatttatta
    40441 atgaatttta ttgacaggtt atatttatat aagtaaacac ttacataata agtatataat
    40501 ctacatatct ataagttgag tcaattcaca agtatatgtc aacttaagtt tatgcatagc
    40561 tcgtgtacag gcaatatata acaaactgcg gtcataatca ctataatatg ttttattatt
    40621 tactgaaggt acaataactt catcaaactc taaacctttt gacattttaa tagaagttat
    40681 ggatactcct tttgtaaagc tagaactttc agaagatatt aagtgaacac tatattcttc
    40741 tcttaacgca tcatatacag ctttagcgtc attatctgtc tttaatataa taccaagagt
    40801 cgcattatca ctctttttaa attcttcaat ttctattttt atcttattta tttcatcttg
    40861 ctcgttatta cattttaata aagctggttc ttcaccatgt cgttcaatag gctctaatga
    40921 agatatgtct tgaatctttt tggcgaaatt aataatctca aaagtagaac ggtaactctt
    40981 gttcaacata actaattccc catcattata tagctgtttc atatcatcta gtgtatgagt
    41041 atgattagga ttaaccaatt gtccaaaatc tccaagaata gtctttttac acttaaaaag
    41101 taagtttatt acagcatatt gaataggtgt atagtcctgc atttcatcaa taacaatatg
    41161 ccttataatc ttatcttctt gtataccttc atatgcagca tgaatatata taaatgggta
    41221 tacatctgac cattcaagtg ttttcttttc tgccatcaca aacatgtttg atatattcat
    41281 ttgtttaaag aaatccttat atagtgtaag agtatttttt attgtcaaca ttccattcaa
    41341 acttttaagt atacttttta cctttggtaa atcttcttcc ataatattat ctgattcaaa
    41401 tttataatga atgtcttcag ctaccttctc taatctcttc ttaactggat atctgttata
    41461 tgcctcaaat ctactctgta tccagtcact tttagctgta aaacttccaa aagtgtagtc
    41521 ttttggaata aaaatcttat ttggcatttg ttttatataa tcatcaataa gttttacaaa
    41581 atcaagtgta gatttaaaac gcactctttc agaccattta gcatcattta tttctaatgg
    41641 gtctttctca ctttcaaaat taataactct atctaattgt acctctgcta tattttcaaa
    41701 acttaattca cataatggct cttctccaag ctcaggaagg acattggaaa tatagtctcc
    41761 aaagacttta ttaggtgata ggataattac attatttgct gatattttat ctttaaatcg
    41821 ataaagaaga aaagcaattc tgtgtaatgc tatagatgtt ttcccagaac cagctacccc
    41881 ttgaataatc agtgtatcag ctttatcatt tctaataatt tgattttgtt ccttttgtat
    41941 tgttgaaatg attgacttca ttttttcatc tgaagtgtga ctaagttctt tttgaagaat
    42001 atcatcttgt atattaatgg aactttctag tgcatattct aattttccat ttttaatttt
    42061 aaattgtcgt tttcttgtta tttctccatc cacccatcct attggagcat catatccagc
    42121 ttttccgatt tcataatcat aaaacatact ggcaattgga gaacgccagt caagtattat
    42181 aagctcatct tcataatcaa atgcaaatct accaatataa tacttagatt catcatccat
    42241 atctttcaac ctaaaatcta ttctagcaaa gtatggagaa tctttgattt tattaatctt
    42301 atctcgaact tttaccataa aagccccata gttatctatt tgttttagtc cttgttcatt
    42361 ttgaaacatt tcatgtgggt ctatttcacc acgatttttt accatataca ttttagcatc
    42421 catataatcc ttatcaagct tgtcaacaag attttcagct ttcttaaaag cattgtctag
    42481 cttgttattt atctctatta gatgtgcaat ctcatctgga aaaaaagtta ataactctgc
    42541 atcatattca cacatattat gtttagaatt agatgcaata atatcgcaat ttactacttg
    42601 tttattttca gacatttaaa tcctcctttg ttaattaagt acttacactg aatcattata
    42661 cttaattgtg ttttggagtt tttaatcatt cttgctattt aacttaaagg aagaataaat
    42721 ttttgagtag gatatccaaa ttccacaagc aattctgctt ctgcaaagcc caacttcttt
    42781 atcatatctc tatgtcctgt atcagctttg tcaccctctc gaaaggtagt aattgaaata
    42841 tctgtatcta tcaagaaatc ttcaataact ttcttcaaaa acacttgtgc aatgtctgaa
    42901 ttacgatact gtggatgtat tccaaaaaaa tcaatacttc tagtttctct attaaacacc
    42961 atatttgcaa tagcgacatt attaagtttt aatattaaag ctctttcttc taaaatatac
    43021 tgttctaatt gatatatata ttcttcctct tgcaaattgg gaaatccatc tatgactaat
    43081 ctcactaaat tcatccataa tggtatgtca gacatacttg caagtttaat ttctttttcc
    43141 aactctagta cattctttga aaataggtta tcttttttca aaacaaatct tagctgcaaa
    43201 ggataaaatt cttgttcttt cctatattta tttggagatt ttttatacat agctttaaat
    43261 atagaagtga atgcctgttg actttcatat ccagctatca atgcaatttc aataattggt
    43321 ttctttgaaa ataccaataa ttttgctgct tctgtcaact ttctccgctt tatgtaatca
    43381 tgcattgtta ggccaacaga agttgtgaaa gttctatgca ggtgatattt tgagtaatga
    43441 acagcattag caactatatc taaatccagc ttttctgata aatgctcctc tatatagtca
    43501 atagcagtta tgacattaat tactttgtaa gacattgcag cttccctcct tttatccatg
    43561 caacattata tcaattatta cttagttata tttaatgaat cttgctattt tataaacaaa
    43621 tttgttgttt aagttttcaa ttcataaatt taatatatta ctttttactt tctgattaaa
    43681 gactctaatc ctttaatttt caataacttt tcatttagta aatctaaaaa gttatttatt
    43741 attttcttct tattagattc cttaattaaa tgataaatat ttatattata cgactaaaaa
    43801 cagcttttta caattattta atatgattac aaacttaccc tatcttaaag tttaatgggg
    43861 tcctgttatg aatcttttta ttttaattta agctcttcat cagtttattt tatatattga
    43921 tgtatttgat ttcatacaaa aaagagtgct tcatgaacta aaaagttcat ttttaaacac
    43981 tctcttttat gtaaataaat ttctattcta ttttgaaata ttataatttg acttataatc
    44041 tattaatacc aaaaataata aattgtacgt ctaagaatag attctaatta gttgctcagt
    44101 agtaaaatct gtttttaact aatcctgctt tgtatgttga acatattcta taattgtacc
    44161 atctggatgt ctcaatgtca tattatatcc tgtaggaact tttttcttat ctcttactac
    44221 aactgctcca ttatccaata gataacttct ccattcatca acagaatcaa ccataaaagt
    44281 agattttgtt tctataaatg gtttaagagc atcatcagaa ccagataata gtaaaacatt
    44341 tcctatttgc gctaattcca acccaacttc tttataggaa aaacgcaaag aacatttttc
    44401 ttttaaaaga ttctcataaa aaggtatggc tttttctata tcttgaatat aaaaacgtgt
    44461 taatatatca tatattttca tattttcccc ctatatatct atataatatt acctctaaat
    44521 tataccactc tacaaattat ataacaataa attaagcata gccttttgag catcttaagt
    44581 agaccttaac aatatattgg ggttacatta gaatacataa attaaatcac cttgatatta
    44641 tttaggataa aatttatgta gtagcagtaa atgttcttct aattatcaac taacaaacga
    44701 taatctttaa ctttctatat aatgggtaac atcattttcg taagatattc actagctgga
    44761 cgattgatat catttaaata ctggtaatat atatcgccat gaagtttata tccattgcta
    44821 tttgcccatg aaaataagtc ttctaaagta gggtcttgta attcataaag tcctaaatca
    44881 attgcagtta ttattttctg tgcaggaaca atcttttgta gaatctcatt tttcccatct
    44941 atataagtgg ctacaggaaa ccctacttca acatccaatt ttactaaatc catgttatga
    45001 aaacatacaa tcggtgctcc tgaagataaa attcctctct cctccagata tttagaaatt
    45061 ttttcaaaac tttgttttga aaactctgaa aattctacca taaagtcaat tgtttttcgt
    45121 attgccaaaa aacagtactc agattgttca gctattgtta tattggatat tctagtcatt
    45181 ttattcctcc attactttat tttcatttct aatcttttta taccaaatta tacgaaaatt
    45241 cactgtgtaa ctaattattt catatgaata taaaacataa cctccttcca tttcttatat
    45301 cgaaagtata gcaaaaataa tctcatcatt cttgtcacac agtgctctta taatcagaac
    45361 atacttaaaa gacttgccta atttttaggc aagtctaaaa gaaatctttt tctataaaaa
    45421 atttatacaa acaattaaaa tatcaaaata tagactaata taaaactata aatttactaa
    45481 tctgaacctc ccatggctaa agtcacaaga ttactgattt atagaatttc gcacactaaa
    45541 atatgtctta ctaattcccc acaggctacc cccctagttc ctacgttctt acataagtta
    45601 tctattatca aacatcaaaa tttaaacaag tttcaggttc atcttctatt cttgctattt
    45661 taaacttaca tgcaattaat actaaagata ctattggatt tataatattt aataaagcgt
    45721 atggaccata tgttagtgca gatacaccta atgttgctgt tatatacgcc ccgcatgtat
    45781 tccatggtac taatggagaa gttaaagttc cactatcttc taaagctctc gacaacatct
    45841 taggatgtat acctctcttt ttatattcct tattatacat tcttcctggt ataactatag
    45901 atagatattg ttcacctgct attatattgg taattgtaca agttaccata gtacataata
    45961 ctgttccaaa tacacccttt gctaatctta gtaaagaaga tgctactact tcaagcatac
    46021 ctgttttttc taaaatacct ccaagagata aagcgcatat tgttaaggat acagtggaca
    46081 tcatattcat aatcccacct ttagataata aagcatctac ctcctgtatt tgtgttgacg
    46141 aaacatatcc attttgtgct gcatctaata tcgtattcat agattctcct tgaaaaacta
    46201 ctgcaaacaa tactcccaat acagcaccta ctataagccc tggtatagca ggaactttaa
    46261 atacaaccaa tccaattact attgctggag ctaataataa tactggtgat aaagtattaa
    46321 atgtagaatc taatgtagac ctaattaatt ctatttgttt tatatctagt gcttgacctg
    46381 aatacttcat tccaattaca ccataaatta ctaaacaaat taataatgat ggtatagtcg
    46441 aatatagcat atattttata tgtgtaaaca aatctgttcc tgccattgct ggagctaagt
    46501 tagtcgtttc agataaaggt gatagtttat ctccaaagta agctccagat attatagaac
    46561 ctacgattaa tccaattggc atacctaaac tttgaccaat tcctaaaaga gctactccta
    46621 ctgtacccat tgtgctccaa gatgaacctg tcgcaagaga tactattgca cagactaatg
    46681 ttgatgctac taaaaaaata tttggtgata aaatttttag tccccagtaa atcatacatg
    46741 gaactactcc acttactatc caagttccta taagtacgcc tattataatt atgattaaga
    46801 ttgcttgcat tgccatcttt atactattaa acatacttaa ttctaactct ttccatttaa
    46861 atccaagtct atatacagcc acaagtcctg ctactgctgc tgctcctatt agtggtacat
    46921 gaggtgatga accatacact aatactccta gacttaaaaa taatatcaaa caagtaattg
    46981 gtatcattgc atcaaacaga cttgcttttc tacattctcg ctctttcata aaatagtttc
    47041 ctcctaatta tttaaacact acactataat tttttttgaa acatatttgc tcgtactaga
    47101 tgacatgata cccccataag ttaataaaaa ttctaaattg tcacctaatt tataatcata
    47161 ttttgtgtta cttacatcta gtatcatgtg gtctgaactt gcccctaata tttttatatc
    47221 agtgtctatt ggaattaaag agtcaatatt tatatcttgt ttacctatag ctatgattgc
    47281 cctttttaag attcccttat cttcataaac aggtttattt ctaaaggcat caactcctat
    47341 ttctccaata ggaactgatg gtttttcttt acattcaact acttggcata ttaatttaaa
    47401 tgcatcttga tatgttccat ttatattttc accgtatgcc gtttctctac caagtagaat
    47461 ggactcgcct attctcaaat tagtaatacc ttctggcata ttattattta tcaacagatg
    47521 aatagaactt gaattacctc cagatactat ttctagattt attccaaatt tttcttccat
    47581 tctttttgct atacttacca gtctgcctat attttcccta gatggtataa tagctccata
    47641 acaggttaag tttgtagcta taccaattat ttcaatattt tttaatctta ctatctctct
    47701 tacattatta aaaaagtctt cttcataaaa gtatccttct cgtaaatcgc ctaagtcaaa
    47761 cataagaact atcttatgaa taatattttt actttcacat aattcattta atttctttat
    47821 tgttcctaat tctgagttaa aactaatatc gcaataattg ataacttctt ctaattcact
    47881 taacattggg attctaatca atatcttagg tagatttata tcttgtaaat tttttaagtt
    47941 ttgtattctt gaatctgcta tatggtctat tccttcacta catatttctt ctactatttc
    48001 tttttgtgca caaaatgatt tagtaacaaa agatatctta atatttcttt catgacacat
    48061 ctgcgaaatt aattttgcat tatgctttaa cttttctata tctatttcta acctaggata
    48121 cataatcttt cctcctcaaa attcaaactt tttaacagga gcttaagaga tgcttcttca
    48181 tattttttag ataaaacacc catagatgcc ataatatagt cattatctat taatatcttt
    48241 gttttttcct ttggaaatag taattcatta tttttattgt atttgagcat attatctaat
    48301 atttcaattc tactagggag cctagtaagt gctccccctg taccaactat atatcttact
    48361 tctgttaaat ctttcccttc tgcaattttg acttttccag ttgttccata gatatttctt
    48421 atttttcctg aatgtcttag catagcttta acaacagctt cttttgtcaa tctttcaaca
    48481 aataatattt cttcttttga ttttggtatt ggaggataat tctctattac tgcatcaata
    48541 tctatagata gttcactttg taagttttct ttacctataa cttctactat attcttcata
    48601 tttacatata cacctaaatc cccctcaaca cttctctttg cagtaggttc aggattaacc
    48661 aaaattttat taatatagtc acttccatct gtaacagaat gtacatcagt agttgctcca
    48721 cctacatcca gagttaataa atctccaata tttttatata aaagtttaga agcttccatt
    48781 acagctcctg gggttggagt aatattttca ttaaccattt ctttaatgta tttcattcct
    48841 ggtgctgtag ttatgtgttc ttcaaataca ctttgaatga ttcttcttgt aggttctata
    48901 ttcaataaat ctattttagg gtatacattt tcagttatat aaagcttata attagtatct
    48961 tcaaatatta accttatttc ctcttgattt tcaacattac cagcatatat aactggtatg
    49021 cctagattca tagaagctat catttctgca ttataaatag ctgtatctct ttctccataa
    49081 tcgactccac cagctattaa aattatgttt gggtttattt cttttatttt atttaaatca
    49141 gttcttttaa ttctacctga tgttatttga cgaataacag ctccagcccc taaagctgct
    49201 tctttagctg cttttactgt catatcatag actaatccat gaacagtcat ttttaatcca
    49261 ccagctgcac tagaagttgc aaacatatca ttatactcta tatcatctac agataattta
    49321 ttagctaaat ctttaatagc acctgataag ccatttctta catcgccacc ttcaaacact
    49381 gttgttggag cttgaccttg acccaaaaat ataggattgt tagtattaat atcgtggaaa
    49441 gcgtttacca ctgtagtagt acttccaatt tcagctacca acacatctat ttttaaagaa
    49501 accttgctat tttccattat ttattactca tttctcttct tttttcaact aagaaagtgg
    49561 ctacatgtat gcctttactg tttcttccaa atccagcatc tatgccttgt tcaacagcta
    49621 tttcaggtgt tacttgtgtt cctccacacg caatcattac tttatctctt ataccttttt
    49681 ctacacaata atcgtgtaat tttttcatgt ttttatagtg aatatcatca tgacttatta
    49741 ttgtagatgc aagtattgca tctgcattca gttcaatcgc tgcatctacc aatttttcta
    49801 caggtacaga agtccctaga tagtggcatt ctatgccata tttttcaata cctccatgtt
    49861 ttatatctat tatttctctt aaaccaactg aatgttcatc ttctccaacc gtagctgaaa
    49921 ctattttcat aggtttttct tctatatctg ctcttatttc ttcatcagtt agtaccttag
    49981 gttcttctgg tattactaaa ttgtttaaat ctatatcaaa cggaactctt cctttcactt
    50041 ctattctagt cccttcagat tcatgcataa cttctctact tatgacctca atttcttgta
    50101 agttcatttt tttgacaaat tccaaagctg caaactctgc tgttctttta tcagtaggaa
    50161 gcatcattgt tatcattata attccatctg cacaccattc catttcaggc ttaattttag
    50221 ttccatttct gtactcatat gattctttta gtctattttc tacattatca aaatcgtcaa
    50281 gctcatcaat atatattatt ttgtctggat tttcaaatgt acatccatct attaacattg
    50341 atggattatc tatagcattt tcatcatatt gagctacatt attgtatcca aagtgagcag
    50401 ttacaggtgc taaataatct tcttctcttt cataaacagt accttcacca actccacctt
    50461 ttatttttct agatattcca tcaccatttc tttctggata atttccagaa tcaacaaaaa
    50521 atccttgttc cacagattca aaatatccac caacttcaga tatttcttcc atatataata
    50581 cagctctttc tttaagttct cttgctttgt ctcttaattc accgacatct ttaagttcta
    50641 tcatatccat aagcccatcc ataccaacta aagcttgctt tgctgtatca catgcttcta
    50701 tattgtatat atgccaaggt acatttctac cttcatcagg tgttatagtt gattgtatgt
    50761 cagctctagt taatactgat gttaataagt ttaatacatg agtaactgtt gcttctctag
    50821 ttgatgattc catatattta gtattcattt gagctctcat cttataatca ctaaataact
    50881 ctctaagtgc aacagcgtaa ggtaagtcta tttttaaaca tggtgctgga ggtgctgttg
    50941 gtggaactgt tgataagcat atattacttt tatctatacc tattttttct gagaatatag
    51001 agtttaaagc gtgttgaacc ataagttcag gcattacctt ccatgcttct ctagcagtag
    51061 cattagcatt atgtgctcca tcaatttgag ctatattggc aaaagccatt attttctttg
    51121 cctcacaagc atctacaaaa gaccttacca tgtttatatt cctatacaga acattatatt
    51181 gtgggtcttg atgtgctcca ttaactcctt cctcagcaaa cattactgct acttcaggac
    51241 cagcaacacc acttacatat gaatgataat taataggtct accaacttct tcttctataa
    51301 aatcaagagc cttcctttga gctcttactt gttttcttgt tattggaact ccacctatac
    51361 cttgtggggt tccttctata agcccatcaa aatgtgattg tccagcagtt ctgataacca
    51421 ttagatggtc agctccatga tgagctgcca ttctcattct tcttatatca tcctcaaatc
    51481 taccagaagc tatttcagta gtaataactg gacttggttg agggtctata ttattgaaat
    51541 acttagatga tggtagccct acactcttct ttaaaggttt tgtacagtct ttatatttaa
    51601 aaggtcccat ttcaagattt tccaagtgtt ctctccaaac ccaacctctt cgttttggtt
    51661 tatatttatc caaatctttt aatatatagt ctatatccaa ttttttattt tcctttaaca
    51721 tgattctacc ccttctctaa ataaacatgt aacttcatcc caatattcac ccttaactaa
    51781 taacaaacct gcttctctaa ccgttatatt tttagattta gccaatttat aaactacatg
    51841 ccctgctcct ttacctataa gacctctttc cataacccct tctaaaatag gttttacttc
    51901 aagagaagaa aatcccattc tcaacaagac agacctttct attgatggtg ttgtattctt
    51961 tttaccaagt tctatcattg gttctactat ctgggtagct aaactccaaa acctttcttt
    52021 taactcatca tcactcagtt cttgaagatg ttttcttcta acttcaaaat catcctctct
    52081 ttttttcatg aacattcctc cattcgtatt gatttaatcg aattttctga atttttattt
    52141 tatattaaat taaaaaacaa taattactat aaattattta atacatcaac aacaaaatct
    52201 cttgaacttt ttacttcttc cattaaaaat tctatatctc gattacttag cttatcaata
    52261 ttatcaatgt gattattaga tatacaattt tttataaatg actttcttat tttattcata
    52321 tcaatatcaa cacatttcaa taattctggt ctttctggta atattaagtt tactcctgca
    52381 atttcttctc ttggatttcc aaattttata tctattccat tttctcttgc aaaggtaagt
    52441 tgtggacata tatgtttacc tgcacccgta tattctgttt cttgtaccac tatagcttta
    52501 tcctcatcta gctcttgagc taaagagaat gctgctgcta aagatgtatt tcctgctgga
    52561 cctctttcta taccttctag ctgtgccaat aattcagttg tataaaaaac ttctccttgt
    52621 tttactaata catatctatc catatatcta agtggtctag ctgcagacct aggtacatct
    52681 gacctatcag gccatgtaca gtatggtatc ccaaaacctg tatgtcctgt tgtaaatgat
    52741 tttttattaa attgattatc actagccatg tgtaatcctt ttaaatctac acttgctcct
    52801 actactttag tattaataga accagctttt atcaatcctc ttgcagttcc agtaagatta
    52861 ccaccacctg catttgtaca aacaactata tcagggtctt ttttatactt ttctctaaat
    52921 tggatagcta actcataacc taaagtttct acacctgcaa ttccaaatgg tgaatataat
    52981 gatgcgttaa aatagcctgt ttcttctaat aaaactaaga acgtataaaa taactctggt
    53041 ccaacagtta actgaacaac ttctgctcca tatgcctcac attttcgcgc tttttctatg
    53101 atttcaggtt gacctacacc attagaatca taacattctt gaactattat acattttaat
    53161 ccctgcattg ccgcttgtga tgctacagct gcaccatagt taccactggt agctgctatt
    53221 acacctttat atcccatttg tttggcatga tatactgcag tagctgctct tcttgcttta
    53281 aaactaccag atgcattcat tgcttcatct tttacaaata ttctagctcc tttaccttct
    53341 ttagcacatt ttctagctaa tttagtcaaa tttttaagct ctattaaagg agtatttcca
    53401 acagcatact gtgattgaat tgcttcaata tcttgtaatg tatatcctgt ttcactcatc
    53461 attttctcat aatcaaaccc tattccctct tgttcaaaag aagaataatc caatccgatt
    53521 gcatttttca ttatttcgtt atttcttccc ataacagctt gataactcat atctttcatt
    53581 gatgttgaat tattcattat atacacctcc aaataatatt tctttagctt ggattcctat
    53641 ttggagtaac tctggtatac atttaccata atcatgagta taatatggat ttattttaag
    53701 taaattacct tttacaagtc tgcctgtaat tgttttaact tctactaagt cgcctataga
    53761 agcatcactt tgtataaaac ctttaaccca catttctaag gatactttct tagtatcctc
    53821 tggaacttgt ggagctcttt cttctggagt caatactata ttgtgtataa taacccaatc
    53881 attaatttta gcatccattt tttcatcccc ttattaattg actattgcct tactaattaa
    53941 tttactgtta ttttaaatta aacattattt taccaattag ctaactgtta tcttactaat
    54001 tagctagttg ctatcttact aattagcctt tgattcttct tccaattcaa cttctatctg
    54061 atttcttata tctcccatta ttgctctagg tactggtata tccaacatag ttttaagacc
    54121 tggtctagca tttattatta atggaataga gtttactatc attgcataag tacctactcc
    54181 tccaggtatt tcaggattta tttgtaaatc aatgtttggt atacctttta ttgatacata
    54241 gtctcctgtt ttaatacctt ctgcttctgg tattatctgt tgtggatgct ccatttcaat
    54301 taaaacttct ccatttacat atccatatcc acattgtcta caaccagcaa catttccagc
    54361 caatgcctct ccgtacttag attttctatc tactgtagtc attattggct cttttgtttg
    54421 ttctatctta tctaatttcc aacctaatcc atcagctatc atatttattg attctacaaa
    54481 acctacatga cctgctatag tcttatcttc tacacctttt ataaattctt cccttgttac
    54541 tccaacacct tgttccacca taacagactt tccaaatgga gataaatcat ttactctctt
    54601 agcttttata ctagtaactt cttcacaagt acctgaaagt gccaatacta gtaaatctaa
    54661 tacaaatcct ggatttattc ctgttccaag tatacttact ccattttcct ttgcaacttc
    54721 atccattttt ttagctaaat ctgcatcatc agcttgtgga tatgccattt gttctgctgt
    54781 tgatataaca tttatttttc tatttaataa gaaatttatt ttatcaaaag cctttttagt
    54841 aaatgaatca gttgctaaca aaacaacatc tacacttttt tctctaaaaa cttcttcata
    54901 atttttattg ataataactt ctggtctttc tcctctttca attcctaaaa catcatacat
    54961 acttttccct gaagtacttc tactacaaac agatactatt tctatacctt ctttctttag
    55021 aatcatatta gccataccta ttcccatagc tccaaatccc catattccta ctcttacttt
    55081 tctcatatat acaccctcct aaaaatttat atattctcaa taattagtta aagcataaat
    55141 cgtgccaact ttaaaattaa agtaaatatt tattggaacg ttgaaactat aattatttta
    55201 ttatgtattt tcataaataa aaaataatat ttcttatgag aaataaaaaa aagagcaaaa
    55261 tattttgctc ttttataata attttgctcc tctattttgc ttttttattt tactttgatt
    55321 ttgttttttt atcttgctta tttataatat tttgatatta tattttttta ttttatactg
    55381 taaattttgc ctattcatac ctaaaattga tgcagcttta gttatattgt aagatgcctt
    55441 ttctaatgat tcatatatat acttttcttc tatctcactg ataatcttac taagtgtttt
    55501 gtcctccaaa tcgtttaaag aataattgtt actctcttta ttctctttaa gagtgtaata
    55561 gtcgtttaga ttaatcatac ttgtttctat ttgtacctca tcttccatca tactcatagt
    55621 acaataaatt atgttttcta gctctcttat gttaccttgc caatcgtaat tttttaaaat
    55681 tttaatagct tcatctgaaa ttcctaccac ttttttattt aactttttat tatactttga
    55741 aataaatttt ttgcacaata gaggtatgtc ctcttttcta tctctaagag gaggaatttt
    55801 tatatgaatt atgttaagcc tataatataa atcttttcgc attttatgct cgtttaaaat
    55861 tacctttgga ctttcatttg tcgttgcaat aattcttaca tctataggta tatccttagt
    55921 tcctccaacc cttcttacat aaccttcttg taaaaccctt aatagtttag cctgtaacat
    55981 aataggcatg gaatttatct catccaataa caatgtccct ttgttagctt gttcaaataa
    56041 gcctggccta tctattgctc ctgtaaatcc tcccttgcta gttccaaaaa gaataccttc
    56101 gaatagtgat tccggcaaag ctgcacaatt ttgtgctata aatggatagt ttctcctatt
    56161 acttccatag tgaatggact ggctaaataa ctctttaccc gttccagttt caccgtatat
    56221 aaatactgaa gcatcagact tgcaagcttt cttagattta gctattgctt ctttaatcaa
    56281 attactttcc cctaaaatat catcaaattc ataacccttt ttttgtctaa tactaacttt
    56341 attgactaaa ttttttttat aatttctatt tagtaaattt tctatcgttg tcatattctt
    56401 tgatatttca agtgccccta caaactcgcc tttaatatca attggtattg tagtattaat
    56461 tgctgttatc tcttttccat ttttatccag atatttttga atattgtctt tgataacttc
    56521 ccttttttcc aaaacttcta atagtgtact cctgtttatt ttcattttgt tgactatatc
    56581 aaaaaatgaa ttggttaaaa tatcgtgtct gttcattttt tccatttttt gtacagaatt
    56641 attgtagatt atagttttcc cgtctttatt aacaaaatgt accccttcat caatattttc
    56701 caatatttca ttcattattt taaaaaatat ctcttggttc aactatatca ccccagtacc
    56761 taaatatata aaaacccaaa aaattaataa aatgttgcta ttgtatttat cttatcatat
    56821 atttatgata ttttttatgt acattatatt aatatatttt taagtatatt taaaatttta
    56881 tttatatatc tttcattatg gttatataca atatttgaat tgtcattaaa tatgccaata
    56941 gttttatata ttattgattt tattaaaaaa agagatatac aaactaataa gttgtaatct
    57001 ctcaaatata aaaaaacaat aaattagctc attggatttt ttagtccata atataattat
    57061 agattattca ccaaacaatc tatagaaatt gtggtattaa tgtttaaata accaactttt
    57121 tctaaaataa cctttatagg aatatatgag ttcataagta aagatagcga aataagaaac
    57181 cagtttggta tgtaaaatct agatttttaa ttaaattatc aagattatat attatagtat
    57241 caaaaataga aaattaaaat taaatttttt ataataaatt gtatacatta taaattatat
    57301 taaattttta atttaagtaa tagtatatgt aatctttagt catcttctta ccagtttttt
    57361 atccactttt ctaatgaagt tattttttca tatgttaatt cctcttgaaa taatgatact
    57421 aaaagacttt ttataaaatt ataacttaaa agtttatgta tttttttgat atgaatttat
    57481 agtacttatc ttctttaatt gagattgtgt atatttaact aaacattaca acaataattc
    57541 tatgaaatca aatcaattat ttacatattt ttttagaaca ataataagta tatattcaca
    57601 aaattttgtg ttaatatttt taatttgtga tataataaaa gcaaggaaac atattttact
    57661 taactttgag tgatgtttcc ttcaacaaat tattttgaag atttactttt ctggaacttt
    57721 atattaagtt cccagccact cttagttggt cgctcagagt ggctctttaa tttatcgtaa
    57781 atgtaactgg ctataacacc agttataata ctcatcatta gattttccat agttttcacc
    57841 tcctctcata agaaagtgaa gaaacttcca tggaaacacc actcatagct ttttcaaata
    57901 tttattcctt gccatattaa ttatatcata aatattggaa acataaaatt ttaatactta
    57961 tcgtctttat tattattttt tataattaat aatttttttt aataatacat aatataaatt
    58021 tatatttctt caataaaaaa gacaatctta attaattgtc ttttttaata tatatttggc
    58081 taatacatta ttttacacaa acattacaag ggtctatttt accttgtgac ttagcttgtt
    58141 ttaatgttac tttatttatt gttttacttc tttttagtgt agaacaattt tttgttagat
    58201 gatatgattt tccattagga gttacatata ctgtactttt atcagttaat ccaggattat
    58261 tagagctagg ctcattactt ggtttgttcg tattatttga attagtatct tttacaatgc
    58321 ctgtatttga atccccaaca ttattagtta tatttaaaca ttgttttata acaacatcat
    58381 caatatttcc aatagcagtt attttacttg cattttttaa tatactctta ttacttccat
    58441 ttccaactaa cactataggt gttttttgtg caattgtaga accaactaaa gaataaacta
    58501 aatcagtccc acttgctatg taaaattctt ttgagccatt ataaaattta tttattattt
    58561 gtttatttgt atcgtatcta tctactccac ctattcttgt tgctttagtt tcatttatta
    58621 acttatcact aatactgact tttccaccaa ttgcataagt ttctaatcca tctgtagcaa
    58681 aacttgtagt gtttccatca gttaatacta ttggagattt atctctaact gctatagaag
    58741 caacactcat agcatctggt tcacctttaa aagcattagt taatattact tttttaacag
    58801 cacttacact tcctatttct tttgcaactt tatgacttgt atctattcta tcagttccac
    58861 ttattctatt tatgcttatt tttttatcac ttaatatttt ctgaactgat gctcctatcg
    58921 aattttttcc tccgattaga tatacttctt ttaccttttc taatcttttt aatgtttctg
    58981 aagggattgt atctttctta gttaacaata ttggggcatt tttagctcct gacaatccac
    59041 ttgctgacaa tccatcagat aatccatttt cagaattaat aagtattgca atatcataat
    59101 tttgtttatc tgctattaaa cctgctgttt catacttatt tgtacctttt attgaattaa
    59161 tagaactaag agcgtttgat gttggacaac tagcaatcaa taatgaaaca gacatcccta
    59221 ttgccaatag tgttttgttt aatttcataa tgaaatcctc ctataaattt tataatatta
    59281 cttaaatatt ataattctac ataaaagaat attttccttt atgaaattag tttatatttc
    59341 aaaattttgt cactttttaa atctatatac taaaaaatag actatttgta acagtctatt
    59401 tcttagtata tcaaatattt atttaggttt acgtttcttt gtactagttt ttgtatatga
    59461 tattccagtt ccaggtatac tcaatgtagc tgattttctt ccttttgaat taactgacac
    59521 tctagcacct ttaacaccag cacttatacc tacactcttc ttacctaaat ttaatttgag
    59581 tcctcctcct agatttatgc ttttcctaaa gttaaatccc atagatataa ccttcccttc
    59641 tatattttaa atatttatac ttaaaattat atttgttata cacttgccat cattggtata
    59701 tctagcatct atatcttttt acgttttttc ttcttatata ctactccagc aactacagct
    59761 gctgttccag ctccaacccc ataagtagca tttctacttt tctcttcttc tcttttttct
    59821 aaaccttcat catatacacc ttttaactta gtttcaatag acttcatttc atctggtata
    59881 ctaaaatctt cttcattgta accttgatta tatacacttt cttttgcttt aggtaactta
    59941 accttaccat tatcatatcc ttctttaaac caattagcat atttaacatt acctatagtt
    60001 gatgtgtcaa atctttctcc tttgatagca ctctcaaatc cttgagtagt gtaatcattt
    60061 tttaattgtt cttgtatttc gtcataagca ttattataag catctataaa tttagaatct
    60121 atgttttctt caaatgtcat tttttcttta tctttattgc tatcctctat ccctttagct
    60181 ttatattctt ctatcttttt agtcttatat tcattgtatc cagttgaata tccggtatca
    60241 taagcatttt ttaaaacttc tttttcatat gtattattag atttagcacc tgtaagccct
    60301 agattatatc cagtctcttt agcctccttt tcttcctctt ctaatttagc cttacctttt
    60361 tcatatcctt ctgagtactt tgattcatat gtatcactat aatctccact ataatttgat
    60421 gaactataat ttcctttata cccatcttca tacccttgat tatatccttt ttctccaaca
    60481 gacctctttt gcttttcttc attattaacc gaagttgctg ttccagaact gcttcctcct
    60541 ccagtataag gacaaccacc attattatgt aaatgagctg gataaccacc acaatgatag
    60601 tgataacttc ctaatccaga tttattttta ttatcacgat gacctcctga tgaatcagtt
    60661 cttcctgaat gagcatacga aaagctccct acactaatca tagttatact cattgcaaca
    60721 gctattaact ttttcttaaa cataatctat ttccccctaa aatattctat gattttatta
    60781 tatcagtcaa tatattatgt ttaaaccgtt aaaattcgac aatgtaaata tttgatgaat
    60841 tgtatttgta aataaatctt ttagcatatc ttgaatatct gtagttgttt tgacatcata
    60901 ttcttcaata agttttttaa tatttcgttt ttgttaaaca taaaaagcgt gcacttttct
    60961 ttgtatagtg atatttgata ttttaattat cactacttta gaagaataca cactttattt
    61021 taaaaaccct gatactatgt tattagaaaa tggtactaat ataaaagata tccagcatat
    61081 actcggacat gctaaaactt caacaactat ggatacatat ttacatgtaa ctaacaacat
    61141 gagaaaccag acggtaaata tttttgaaat cattgtagaa taaatttgcc acccaaaaat
    61201 aattatggtg gcaaataggt ggcaaatcta cttttaagac acactataaa agtctgaaac
    61261 cctgttattc tctaataagt ttaactacag tctcacaatg tggcgtattt gggaacatat
    61321 ccatacactt aaccatctta atctcataac catacccctt aaaatcaacc aaatcaacaa
    61381 ccaaagactt aggattacaa gaaatataaa caatctcctt agcaccaaat ccacaaatat
    61441 ccctaatagc atccttatga atgcctggtc taggagggtc aacaataatc aaatcaggct
    61501 tgtcctttaa atccttaaca accttagcca catccccagc aataaactca caattagtaa
    61561 gtccattcaa cttagcattc tcattagcag caacaacagc ttcctcaact atctcaatac
    61621 catacacttt cttagcagca ccagccataa cctgtccaat agacccagta ccactataca
    61681 agtcaaatac aactttatca ttgtaatctc caataaaatc cctagcaata ctataaagaa
    61741 cctcagctcc cttagtattg gtttggaaaa acgaaaatgg tgaaatctta aacttaagac
    61801 ctaaaatttc ttcttgtata taatctcttc catacaatac tcttaactca tcacattgga
    61861 ctgcatctgc taaaccatca ttaatagtat gaagtacacc tactaactca gcttttaaat
    61921 caatccctaa aagcatatct ttaaactcat acatatcaaa atcttcttga gaagaagtta
    61981 ctatattaac tataatctca ttagtgtgaa tcccttttct aactacaaga tgtctcaaat
    62041 atcctttgtg attcattgtt ctataataag gaagcttttt ttcattgaaa aactctacac
    62101 tagaaactaa tatctttgag aagtcttcat caaccaacat acactcctca acagtttgta
    62161 tatctatatg ctttcctttt ttatgaagac ctaatgtcaa aggtccattt tttacttcat
    62221 ctccaaaagt atattccatc ttatttctat aatatttgtt ttctggactt ccttctatcc
    62281 ctaaaaactg aaatccaaat aaatcttgct ttaaaaataa atccataact tgtttttctt
    62341 ttatttctaa ttgtttttca tatggcacag acaatagtgt acatcctcca cactctctaa
    62401 agtgcttgca agtttcttca gtttcaagtg gtgagttctc taataattct atcatcttaa
    62461 cttcagcttt cttacttcta acttttttaa ctccagctct caccttttga ccacttattc
    62521 cacccttcat atggataact ctatctccaa cttggcttaa agatgtacca ccaaattcca
    62581 ttttatctac ttcaaattct ataacatctt ttttcttcac agacattcct ccaatttact
    62641 atagtatctt tattatcttt cttcaatcaa ttttacttct tcatctgtta actctctata
    62701 ctcacctaac tctaaacttt catccaattt taaattgccc atagacaatc tttttaagta
    62761 tactactttt ttgcctacgc tttcaaacat tctttttact tggtgaaact taccttcatg
    62821 tattgtaagc tctatttctg attcatcatc actttttaat atatttaact gtgatggcat
    62881 agttttatat ccgtcatcaa gcactacacc ttctaaaaaa gcttctacat cttcttcagt
    62941 aactactcca tcaatttttg cataataagt cttaggcaca tgcttcttag gagataaaac
    63001 tctatgagaa agctttccat cattagttaa aactaataat ccctctgtgt ctttatctaa
    63061 ccttccaaca ggaaatggtt caaaagctaa gtatgataaa tcaattaaat ctaatactgt
    63121 tgggtcatat ttatctgtag ttgctgatat atatccatca ggtttattca tcatcaaata
    63181 tatatattct ctatatataa cttcttctcc attaaataat atctcatcat tttcagtgtc
    63241 tacttgagtt cctggatttg aaacttcact tccatttact actacagacc cttgtttaca
    63301 atacttcttt atttcacttc tgcttccata acctaaattt gaaagaattt tatctatacg
    63361 ttgcttttta gccattaaaa atgctctcca ttctcataat atttttcttt ttcaaatact
    63421 gtaaatgcta aatccatact ttctacatct aaattatttc ttatactatc agttattatt
    63481 cttgctacta tatcttgtac ttcttgtcct ctatcaaacc aagctacttc aacaaaagga
    63541 tatacatctg ctattttacc atctcttatt gcaactgact ttatacattc tatttcaaag
    63601 tagtctcttg gacattttac agcttcaact aaatcattta tcattttttc acttatttta
    63661 catatatcat tttcattaat tcctcttatt tttatctgtg gcatagttta tctcctttat
    63721 tttaaatttt tagtgaatta tcatccactt aattcccttc gataacgttt gaagtggtaa
    63781 cttcttggtc aagacaacta ttgttatcag tatctccaaa ctaataaggt agttcctacc
    63841 cttatagtta gcattatata gctaattttc ctaaattgtt tatattcttt attattttat
    63901 ttaatcctat tgtcttctat ttaacattat acttgtaatt tatagttatt acaatttact
    63961 atatactttt tttgatttaa cgttataata taagtgatgg gtaaatttta aaagttaata
    64021 ggaggagttt aaaatggcat taattactac aaaagaaatg tttaaaaaag cttacgaagg
    64081 tggatttgct ataggtgcat ttaacataag tgacttagaa caattacaag gtgttttaaa
    64141 agctgctaaa gctaaaaatt cttatgttat gatacaagct tctatgtcag ctgttaaata
    64201 tgctggacct catacattag ttgaaatggt taaggctgct tctgatgaaa taggtgtaga
    64261 tgtagctctt cacttagacc atggtccaaa tatggatgct attaaaactt gtattgatgc
    64321 tggtttctct tcagttatga tagatggttc tcattttgac tttgaagaaa atgttagaat
    64381 aactaaagaa gctgttgaat atgcacactc taaaggtgtt gttgttgaag cagaacttgg
    64441 tgttctagct ggaacagaag atgatgttac ttctgatgtt cacaagtata ctcaaccagc
    64501 tgaggcagtt gagttcgttg aaagaacagg tgttgattca ttagcaatag ctattggaac
    64561 ttctcatggt gctttcaaat tcaaaggaga agctaaatta agattcgata tactagaaga
    64621 aatccaaagc aaattaccag gtttcccaat agttcttcat ggtgcatctg ctgttgacca
    64681 aaacgctgtt gctacatgta atgaatttgg tggaaatata gctggtgcta aaggtgtacc
    64741 tgtagatatg ttaagaaaag catcttctat ggctgtttgt aaaataaaca tggatactga
    64801 cttaagacta gctatgactg ctgctatcag aaaattctta gctgaaaatc caaaagaatt
    64861 tgacccaaga aaatacattg gtgctggtag agatgctata caagctgtag ttgaaagcaa
    64921 gatagatgat gttttaggtt ctgctaactc tataaactaa tataactttt aaatatttat
    64981 aaaagaaaag agatgactta aagtaattaa atttattttt aagccatctc ttttaagttg
    65041 tataaattat gtatcttata atattttaat tatattgtct ttttaaactg acagctaaac
    65101 gtgaatatgt ccactcttta tctttctatg gatggtagaa ggatatatac ctataacttc
    65161 agcagccttt gttattgaac catacttatt tatagcctta cttatcatta tttggtccac
    65221 tattttatat gcatcattaa gattcataat tccatttacc actattggtg ataaatcatc
    65281 tgtttcatta tcaatcatat ctaaatttat cagcatgtta aattcatctt ctccaattac
    65341 atttttagct gataatacta caaatctttc aatgatgttt tttaattctc ttatgtttcc
    65401 tggccaagaa tattcttcta ataactcaat aactttaggc gaaacttcta cttctctatt
    65461 gtacttttca ttgtaaagtt ttaagaaata atttactaat ggtactatat cctcttttct
    65521 ttctctaaga ggaggtattt ttactggaat aacacttaat ctatagtaca aatcttgtct
    65581 aaatttaagg ttatttctaa ggtcttcatg agaaatatta gtagcactta tatatctaac
    65641 atctattttt ataggcttac taccacctat tctagtaata gtattttctt gtataaccct
    65701 aagtaatttt ttttgcatct gcattggtaa ctcacctatc tcatccaaga aaatagttcc
    65761 accattcgcc tcttcaaaaa gtcccttttt accttttttc ttagctccag taaaagaacc
    65821 ttcttcatat ccaaaaaact ctgactctaa taattcattt ggtatagctc cacagtttat
    65881 agctataaag ggtttgtctc tccttgaact atacttatat attgttcttg ctattatttc
    65941 ttttcctaca cctgattctc catttataaa tatagaagaa tctgaggctg cagctctctt
    66001 aacaactttt tttatattgt ccataatctt actttgactt atatactctg tgtcttctga
    66061 tatatcacta tattttatct gagattcaag cttaataacc cttgaaatat ctctccctgt
    66121 tagtactcct cctgtaagct ttcccttaga atcccatctt ggaactgccg tatatgcaat
    66181 aagcttccca tctgggtaaa cgatatttct ataaatcttt ttcttcactt taagcatgtt
    66241 taatattatt ggttcttcaa ctaatccttg tctcacaagg tctgttatat ttctaccaag
    66301 tacctcagtt cttttaaaag gtaatatctc atcactgaca gtgttcatct tttcaattac
    66361 cccatctttg ttaaacacaa caacaacttc atctatatgc tctattatat cttcaattac
    66421 tttatcagat attagagact tctcaaataa ataaactata ttattttcat tattattatc
    66481 tttcatattg tatatatgtg cagtcatcct taactctctg aaagctattt tctttttact
    66541 atctgtcagt atttctgttt tgtcaaaatt tgaatctaat tcatttatat ttctaggctt
    66601 ttttatatct agaaaactaa aaatactatc tattagatga ttacagtata ttatttcgcc
    66661 tgattcttta cataaaatgg ctggattttc tacatgttct ataagcccta ttaaattatt
    66721 atccattaat attcgcacct ttcattaagt aatttattga actacataca aaatttattg
    66781 tttgcttaca ataaatacca tcagcacata taatcaactc cttataatat tcgtcttttc
    66841 aagtagttta tttcattaaa acgtaactaa gatataattt taatgattat agtttgttaa
    66901 aaatttcatt ttgcttttaa ttgtatttta tcacatattt agtaattatt tgtctattta
    66961 atacaattta attaaattta agatttattt aaaaaaacaa agataatcct tacagaatat
    67021 aactcacaat ttgtaagcaa tatacatata aggattatcc agattatctt gtattataaa
    67081 attttattca aacagacttt ttaataactg acaaatacac actagcaata acttatcaaa
    67141 gggttaataa ttatgttact aactaataag acttattcaa tcagtaatat ttatatagtt
    67201 tgtatctatt aagcttttat agcttctaat aaatcatcaa cgctatctaa tttttctata
    67261 agcattggaa ctatttcatg taagtctcca actactccat agtcagcgat ttctaaaatt
    67321 ggagctgctg ggtttttgtt tatagcaatt atgaaatctg atgattgcat acctgctaaa
    67381 tgttgtattg ctcctgatat accacaagct atatataggt ttggtttaac agtagttcct
    67441 gtttgaccta cttggtgaga atggtctatc catccagcat caacagccgc acgagaagaa
    67501 ccaactactc cacctaattt gtcagctaat ttcttaagca tttcaaatcc ttctggtcca
    67561 cctaatccta aaccacctga tactataaca tttgcatctg taagagatac taaatctttc
    67621 ttagttttaa ctgtttctaa aacagtagtt cttatatcat cttgagttat tttgtagtct
    67681 aatgctataa cttcaccagt tcttgtttcg tctttttcag ctttatccat aacaccaggt
    67741 ctaactgtag acatttgagg tctatggttt ggacaaatga ttgtagccat tatgtttcca
    67801 ccaaatgctg gacgagtttg ttttattttc ttatcttctg ggtctatttc taacttagta
    67861 cagtcagctg ttaatccagt tccaactctt gaagctattc taggtgctaa gtctctacct
    67921 atatgagttg ctccgaaaag cattatttct ggttttattt catctattgc atcttttatt
    67981 acttttgtat atgcatcagt tgtatatttt tctaaaagag catcatctgc aacataaact
    68041 ttgtcagctc caaaagtgat taattcttta gctaatccat caacatcttt tcctaataat
    68101 attgcacaaa gttctgcatc tacttcttta gctaattttc ttccttctcc taataattct
    68161 ataactactg gagctatttt tccttctctt tgttctgcaa atatccatac gtttttataa
    68221 gaacttaaat cttttatatc attcatagtt tgtactcctc caacttatat aatgtgcttt
    68281 tgttttagac cgattattat tttactagct atttcatcag ctttaccttc taagatttct
    68341 ccttttcctc ttggttcagg agtaaatgat ctaaatactt tagttggtga tgcttttaaa
    68401 ccaacttctt ctttatttac atctaaatca tcaattgtcc aaacttttac atcatgttta
    68461 tatgctttta ctattttatc aacagacatg tatctaggtg tatttaactc ttttactgct
    68521 gttaaaagta caggtgtact tacttttatt aattcgtatc catcttctaa ttgtctttga
    68581 actattatat cttttccttc tattttgaaa tcttgtacat atgtaacttg aggaatgtct
    68641 aatttctctg caatttgtgg tccaacttgt gcagtatctc catctattgc ttgtcttcct
    68701 gcaaatatta tgtcatagtc acctacttta gatattcccg ctgcaatagt atttgatgta
    68761 gcccaagtat cagctcctcc aaatgctcta tcacttaata atattgcatc atcagcaccc
    68821 atagctaaac actctcttag cataaagtct gcttgaggag gtcccattgt tataactgtt
    68881 atagttacat catcatgctc atctttaatt tttaaagctt cctctaatgc atttgcatca
    68941 tctgggttta atatacttgg aactccatct cttataagag tacctgtttc tttatttatt
    69001 cttacttcgt tagtatctgg aacttgtttt acgcaaacta atattttcaa agtctatacc
    69061 tccaatttca ttagattatt tattatttta atacgtttga tgaaattacc attttttgaa
    69121 cttctgatgt tccttcgtat attgatacga ttctagcatc tctgtacatt ctttcgattt
    69181 cataatcttt tataaatcca tatccaccgt gaagttgtaa tgctttataa gcaacttcat
    69241 ttgcaatttc tgatgcatag tatttagcca tagcagattc ttgagcaact ctcattcctg
    69301 catctctctt ttgtgctgca tcataaacta atcctctagc tgcacaaact tttgtttcca
    69361 tgtcagctat agtaaattga gtattttgga atttagctat aggcttacca aattgaactc
    69421 tttgttttgt atatttaaca gcttcatcta aagcaccttg agctattcct agagctaagg
    69481 cagcaacacc tattctacca acttctagag tacccattgc tatcttaaat ccttgacctt
    69541 cttttccaag taagttttct tttggaactt taacgttttc aaatattaag tcagaagttt
    69601 cagttcctct tatacccatc ttatcttcat gagctcctgt tgagaaacca tcccatttac
    69661 tttctactat aaacgctgat gttcctctaa gtcctttact tctatctgta actgctatta
    69721 ctatagctac atcacagaat ggtccattag ttatgaaagt ttttctacca tttaatatat
    69781 agtggtctcc aactaattct gctgttgttt gttgtccacc tgcatcagaa ccagctcctg
    69841 gctcagttaa accaaatgct cctattaatt cacctgttgc tatacctttt aagtattttt
    69901 gtttttgctc ttcagtacca ttgtaaagta ttggtaatga agcaagagaa gaaccagctg
    69961 taacaaaagt agctgttgaa gcacatcttc tagctatttc ttccattact atagcgtaag
    70021 aaacatatcc tgctccccca ccaccatatt cttctgggat attactgctt ataaattttg
    70081 catcagctaa ttttttgatt aattccttag gcattacacc tgttttatcc atttctgctg
    70141 gtaaagtatc taattcttta ctaacaaaat ctcttactgc ttttcttaaa agttcttgtt
    70201 ctttgttata taacatagct tcctccaatt ttcatatttt atagtaaatt tattttaatg
    70261 aacttacgct aaactcatca tctcagcaaa agtttgaata cgagttctgg cttgttcatt
    70321 attctgagtt tgttggtcga tttcaacata taaagtaggt attccactat cttctatatc
    70381 ttttctaact aaaggataat cgtattcttc tgggtcacag aatttcatca tacagaagat
    70441 aacaccatct atatctttct ttttaacttc atctactata agtgacccac gttttttctt
    70501 agggtcataa gctaatgaac atccttctat gtttgaccat tgtcttgcta atctctctaa
    70561 cgcatcatca cctgctggta catctgttct gaattgtctt gtttcttgtg ctaagtcgtc
    70621 agctacaact gatatattgt tgtcttctaa aatgtctaat atatctttag aatcagctaa
    70681 tatacctgtt aataaaactt tctttccaga acagacttct tctggcatag catttaattt
    70741 tgctattaaa tctttcacta gctcagtatg ttcttctttt ctcataaaga acccactctt
    70801 aattactaag cttcttattg atggttttat agtattagaa tgtttataag ctacttcaac
    70861 aaagtctctc atagtttttc tatgttcatt gtaaacttct atactttcat gaatttttgc
    70921 ttcttctatt tcatatccac aaatttcttc aagttctctt tttacacctt tgtactcact
    70981 gattaagtat tttacaccag cttcaagttt tctattttgt gggtgtacta atgatatata
    71041 ttttatatga ggtactgctg atttccagtt ttgtcctaaa caaattagtg tatcacacat
    71101 acctggtata ataactccag ataattcatc ataagcacct tttaatccat attctaaaca
    71161 tgattgcatt attgaacatg caaatgcagg gaaatattgt ttagctaaat ctaattctgt
    71221 ttgtcctccc catataccaa ctggaagcat tccagctgca tgtataattt cttctgggca
    71281 ataaactggg aaacaaccta tagctttttt accagtttca cttttatatt tttttacagc
    71341 cgcatttgga ttttcaacta cttctttcat tttagataaa atagcttcca ttatatctca
    71401 cctctattaa gtttttttct ttcttccatt acttcaacta aaccttgaat tcttgtttca
    71461 aattgagcat tagtgaaagc tctagggtct gcttggtcac catcaaatcc agcatatggt
    71521 aatccagttt cttcagctgc tcttctttgc atttcatatt gtattaaact cataagttta
    71581 cagcttctgt tcatatgata gaatgctcca tcacatttac cctctactaa agaatcaact
    71641 ctatattttg tcatacggtc taggtttaca ttgttaaaca tagtactata tgctactgcc
    71701 attccatcta aatcattaac ttcatattgt aatgcccaag catgtgggta aacactacct
    71761 gtcatgttaa ctccaaattt agctaatgtt ttcatcttgt atcctatata tggccaacaa
    71821 ggtatacctt ccatcattat tctgtatttt tcttcccctc tgaaagaaga tttaccagtt
    71881 ttcatgttgt cctctaattc ttctataagt aacttaaatg cttctgtagt ttctttttta
    71941 cctctagcac aaactattac agccatgtaa gtaaataagt caaatccatt cattggagaa
    72001 ggagaagaat ctgctggtaa actcatagaa tacttccata gtcttccgtt ttcagctgat
    72061 attttcatta cttcttcaaa cttcttaggg tcaaatttct ttcctgatat aatttctagt
    72121 tgttttatag cttcttcaaa ttgagcttta atataatcta ttcttgattg agtaacttcg
    72181 tcttcattat tgaaagttgt atcaatcatt attaaaggta tatctaattc tcttgaaata
    72241 ttttcatacc attttataac ttggttacat atattgttac agcaaagtag gaaatctgga
    72301 gccggcatat ccaaagcttc acatccacca ttttctaaaa gaccaaaatt tgttcttgca
    72361 tatgcacata ggtcaataga atatccttta gattcagcta tttcacataa ttctaaagaa
    72421 ccttttttag ctgctactcc agctgcttgg ttttctggat ataatacgtt taagtcaaat
    72481 acttctgcta actcttgagg aaatactgat gttgaccaac ctacaggtct tccttcttct
    72541 ttagctttaa atgcatttgc atattgttca gctaataaat cattaattac tactctagct
    72601 tctttttttt cagacatttt catccctctt tttatatatt tttcacttct ttttgtgatt
    72661 cttttgcttc atcaaaagca tataacgctg ctcctaaagc accagttaat tgaggtatat
    72721 caggtacaat aatttctgtg ttgatttctc ttgccatagc tcttacaata ccactatttc
    72781 tagcaactcc accaaccata actacatttc tttgtactcc tattcttttt actaggctag
    72841 aaactctctt tgctactgaa gtatgaatac ctgccactat atcttcaatt tttgcatttt
    72901 cagataaatg tgatataacc tcagactctg caaatactgt acatgtactg cttattgata
    72961 cttcattttg agaattcata gatatacttc cgagttcaga tacatcaacc tctattattt
    73021 ttgccattac atctaaaaat cttcctgtac ctgcagcaca tttgtcattc ataagaaagt
    73081 ttaatagtct tccattatta tctaatttta atacctttgc atcttgacca cctatgtcaa
    73141 taatggttct tgtctctgga attatgaaat ttacccctct agcatgacag cttaattcac
    73201 ttatttgctt atcagcatct gaatagttca ttcttccata tcctgtaact acaacttttt
    73261 taatatcttc tcttgcaaga cctgtcttgc catataattt ttctaaaact cttgatggtc
    73321 cagtagtccc agtaccagag gatattgttt cagaagctac aatatcttcc ccattcttta
    73381 agattactcc ctttgatgca gttgaaccta tatctaatcc cattgtgtac atctttagca
    73441 ttctcctttt aaaaaatcta gaattactaa ttaatatctt acaactttac tatctttaaa
    73501 gttatttatt ttctcttcat cgtagcctaa agattttaat acttctacag tatgttgacc
    73561 tacttttggt gctggtgtat attctttaat tccttcattt ctaaacataa ctggagtatt
    73621 aactaagaca cctgtatttc cgctatcgta tgttttctta aataagaagt catttgccca
    73681 agcttgttcg tcatctaata aatcttcaca gctttgaatt ttttcaaatg gtaagtctgc
    73741 ttcttctaat aaagctgacc actcgtctaa tgttttttct aacatagctt ctccaactat
    73801 cttaactaaa tcttcaacat gattaaccat tgaatctatg ttattatatc tatcgtcttc
    73861 taatatatat tctctattta taaccttaca gaatttgcct aaccacttgt tgtattgtat
    73921 taaagctaat tgaatccatc ttccatcttt acatttatat gtagtcatta atgggctgtt
    73981 tggattttct cttgataaag gcatttcatt tccgtattgt gctgttgtta tcattgttcc
    74041 cattccatat atagctgtat ggaaaagact tactgttact ctctcacctt taccagtttg
    74101 agctttttta tgtaatgctg ctaaacttcc tgctgctagt gctagacctg catagtggtc
    74161 accaaatcct gctgctgtat ttgctggaga tgttcctttt tccataacag attggctaac
    74221 tcctcctctt gcgaagtatg cagtatagtc aaatcctggt ttatctttta aaggtccttt
    74281 ttcaccatat cctaatattt gagagaatat taatcctgga tacttatctt ttatttggtc
    74341 ataagctata cccatttttt ctaatgcttg aactctaaca ttagttacaa atatgtctgc
    74401 ttctgataat aatttatgta atatttctac tccttctttt gattttacat taatacttac
    74461 acccttttta tttccatttt ctaattcaaa catagggttt tcatcatctg atgctggaga
    74521 tttaaatgtt ccacccataa ctcttattcc atcaccttct ataggttcaa tcttaataac
    74581 ctctgcaccc cagtcaccta acatttttgc acaacatggt gctgcgatga aacttgaaag
    74641 ttctactact ttaactcctt ctaaaagcat aacgttttcc tccttaatag tatcaattat
    74701 aaaatttcga ttaaatcact cataaaatac ttttttctga tattaactgt ctgatgtcaa
    74761 agttcctaat cttatctaaa ataacttaaa aattattaaa attatttttt attgttttat
    74821 tgtcttacac ctatattgtt aagcatttat cgtgccaact ttatatttaa tcaaaatatc
    74881 gcttgatttg aatgtttttt aacaatatgc aattgaataa tctttttcat atcgtggcat
    74941 cttgccacta tccattgcat tttgctattc tttctatgct tataaaagtt ttttttatat
    75001 atttttttat gaaaaagctt gacatttcaa aaaaagtttt cattgctaaa ctcaaaaaca
    75061 catgttatca ttgtaattaa tcagcaagtt agttaactaa tttcaaactt agataatttt
    75121 ttaataatat ttaatctaaa aaataacaat ttctttgata aattttagag taattattac
    75181 attaaataaa aaattactaa atatcaaaat aacacaggga aaacaatttc aaaactaaat
    75241 ttttaacctt aaaaattttg tataaaaaat ataaccttat atgctacata ttatttctaa
    75301 atgaaaaatt accataataa tttaataatt acaggagggt attgatatga aaatactagt
    75361 atttggagca cgcgattatg aagaaccagt aataaaaaaa tggtctgaag aacataagga
    75421 tgttcaagtg gatatttatc ctgaaaacat gactgaagaa aatgtagtta aagctaaagg
    75481 gtatgatggt atatctatac aacaaactaa ctatatagat aatccttata tttatgaaac
    75541 tttaaaagat gctggggtta aagttatagc ttcaagaact gcaggggttg acatgataca
    75601 ttttgattta gttaatgaaa atggacttat cgttacaaac gttccttctt attcacctaa
    75661 tgcaatagct gaattagctg ttactcaagc tatgaacctt ttaagaaaga ctcctctagt
    75721 aaagaaaaaa gtctgtgaag gtgattaccg ttggatagct gaacttcttg gaacagaagt
    75781 tagatctatt acagttggtg ttataggtac aggaaaaata ggtgctactt ctgcaaaatt
    75841 attcaaaggc ctaggagcta atgtaattgc atttgaccaa tatccaaata gtgatttaaa
    75901 tgatatatta acttacaaag attctttaga agaccttcta aaagaagctg accttataac
    75961 attacatact cctttacttg aaggaacaaa acatatgata aataaagata ctctagctat
    76021 aatgaaggat ggagcttaca tagtaaatac tggccgtggt ggtttaatta atacagggga
    76081 tttaatagaa gcactagagt caggaaaaat tagagctgct gcccttgata catttgaaac
    76141 tgaaggattg ttcttaaaca aaaaaatgaa tcctggagaa ttaactgacc cagaaataaa
    76201 taaacttctt tctatggaac aagttatatt cactcatcac cttggtttct tcactagtac
    76261 agcgattgaa aatatagttt attctagttt aagtagtgct gtagaagtta taaaaacagg
    76321 aactgctact aatagagtaa attagtattt tataaatata ttttttcatt aattagctat
    76381 tatctaacaa aatagccttt aatttcccaa atataagaga gtcagctcag tatatattga
    76441 gtagactctc ttatattatt ttttatatta aacttgtact cttcttattc ataccaaatt
    76501 aaatttatat taacttactc taactactcc tactctatac attttaccta tttttacttt
    76561 tctaattata tacatttgaa tgggtacaat ctgtatttta caaaaaaaac acctaacaaa
    76621 ctatgaaaat tgtatgatta atattaatat atttataaga aaacataaca attatgttag
    76681 ctctggattt ttagggaggg agtatattgg aaaaaatgaa taaagttact tctaaaaaaa
    76741 ctacctatat tgttacttca gcattatttg catccataat atgcctgaca atagcatata
    76801 tattacatat tccagttggt ggaaacaatg gatatgttca tataggagat gcttttattt
    76861 atctagctgc aactattttg ccaacaaact atgctatagc tgcttctgca attggtgctg
    76921 gtctagctga tttatctaca ggtgctgcta tctgggtaat accaactata atcataaaac
    76981 caatacttgt attatttttc acttctaaaa gtgacaagat aataaataag agaaatatag
    77041 ttgcttcggt tgtagcaggt atagtaggat tagttttata catgtttgct gaaggtataa
    77101 ttattggaag ttttacaagt gcatttgtaa tgagcttact tggcttatta cagccaatag
    77161 gaagttttat tgtttttata atcttaggta tggcacttga taaacttgat tttaaaaaac
    77221 gttattttaa ttaagaattt aaatttatgg agggaaaatt atgaatatct tatatacaat
    77281 tgaaaatagt atctatgtaa atataactaa tacatgtcca tgtagttgtg ttttttgtat
    77341 aagaaacgaa aaagatgaag ttgcaaatag tggtagttta tggttggagc atgagccaag
    77401 tgtagatgaa gtaaaagaag ccttcaataa atacaattta gacgactatg atgaaatcgt
    77461 attttgtgga tatggcgaac cattaatgag aataaatgaa ttgatagagg tagcaaagtt
    77521 tataaaagaa aaaagtagca taaagataag aataaataca aatggtctaa gtgatttaat
    77581 acataacaaa aaaacagcta ttctgttaaa aaatgttata gatgcagtat caataagtct
    77641 taatgcccca aataaagaag cttataatag agttactcaa ccaaagtttg gagaaaaatc
    77701 atttgattat atgttagatt ttgcaagaga ttgcaaaaaa tatataaaag aagtagcttt
    77761 ttcagtagta gatgaaatat ctccagaaga aatagaagaa tctaaacaac tggcaaaaaa
    77821 acttgatata ccattaaggg ttagacataa gaattagcaa gtaaaattat aaaacattaa
    77881 gaatcacctg aatggtcttt caggtgattc ttcctcttgt attaacattt tacaaaatag
    77941 atttcatacc acactaggaa tgtgtaaatc atccaaatct ttctccaatt atcagagttt
    78001 ccactaatgt agtcattaaa aatctcatta atctcatcta tgttaaagaa tttatctgct
    78061 atctcactat tgaatttatc tcggatatcc ttattatatt ggtcatcagc catccatata
    78121 cgaattggca caataaatcc taatttattt ctaaaagcaa tctcttctgg taatgttttt
    78181 gatgcagctg tacgaaaggc aattttattc tcttttcctt tcactttata ttctgatggt
    78241 attctggatg caatatcaaa tattcttgta tctgtaagag gcattctgac ttccaatcct
    78301 actgcagtac tcattttatc tatattaaaa taaatatctc cttccaacca tatttgaata
    78361 tctacatttg acatgttagt aagtgtatct agttcttctg tttcttcata aattcctttt
    78421 accacttcaa taggaagcac atcaggattg tatttcttaa gaatttttct tttctcaccc
    78481 tcattcataa tgtttgtatt tccaatataa aatgtcttca aattctctcc ataacactct
    78541 gccttatggt atatattata tccaccaaaa aactcatctg ctccttctcc agagtaacag
    78601 atatttgtgt tttcagctgc tgccttgcat ccaatagcaa aaacgatagc agatgcatct
    78661 ccagttggtt gctccatatg gtacattaca taaggtactg acgcaaaata ctcttcaggt
    78721 gtaattatat atctcgaatt tttacaccca agaatattag ctgtctgttg tgctaatttt
    78781 gattcatcaa aacgctcttc ctcataacca catgaacctg ttttttctat atctgacatt
    78841 gccaatatat aagatgaatc aacacctcct gataggaata actctgcagt ttcattctct
    78901 gattttacct ctggcataat tttctgaagt gttgtatgaa tttcatccgt ccattcctca
    78961 atagacttgc tgtggtcagg atgaaattca ggtttccaat aacgaccaat acatactgtt
    79021 ttattctgat agacaaggta atgtcctggc ataagcttct ttactccacg gaagaatgta
    79081 ttttcacctg ctacatatgt gaaagacata taaatctgta acatgtcttc atttaactct
    79141 ttcacaaatc ctggttgctc cataatctta cgaatagatg aaccatagag aagcttccca
    79201 ttttcagtct cataataata gaaaggtttt accccaaact ggtctcttag gcagaagagt
    79261 ttcttttcca cagtatccca taatgcaaaa gcaaacattc catgcatgtg atttgccata
    79321 tcatatcccc atgtcttata tgcttccact aaaattgctc gttcacgctc atctctacat
    79381 aatgaagaat cgattccaag ttcttcacaa agaactttcc agtttcgaat atgcccattg
    79441 tattctagaa tttttatcat atatgtgtct tttcgtgtta aatacttttc ttctaaattt
    79501 atcatatgtg tatcctttcg tattaaaaca tttaacttct aaatttatta aatgtttata
    79561 tatatttatt cacttataat cactgaaaaa tcgacttttt tcaaagtaaa ttctttttga
    79621 attagtacta atctgctctt tattaatggc ataaatatga ttatcaagta ttatcagtgt
    79681 tataacattg aattttttat tcttcataaa tattgccccc tttatattac caactaaata
    79741 taatatatct tatgttactt acatattctt taagaaattc tg
//
LOCUS       DAMJWS010000006        77078 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMJWS010000006 DAMJWS010000000
VERSION     DAMJWS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 77078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.006"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            784..2454
                     /locus_tag="PC399_000419"
     CDS             784..2454
                     /locus_tag="PC399_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF2591298.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHKMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESSKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2576..3310)
                     /gene="pflA"
                     /locus_tag="PC399_000420"
     CDS             complement(2576..3310)
                     /gene="pflA"
                     /locus_tag="PC399_000420"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF2591299.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3470..5701)
                     /gene="pflB"
                     /locus_tag="PC399_000421"
     CDS             complement(3470..5701)
                     /gene="pflB"
                     /locus_tag="PC399_000421"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF2591300.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5994..7055
                     /locus_tag="PC399_000422"
     CDS             5994..7055
                     /locus_tag="PC399_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF2591301.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7146..8759
                     /locus_tag="PC399_000423"
     CDS             7146..8759
                     /locus_tag="PC399_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2591302.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9034..11019
                     /locus_tag="PC399_000424"
     CDS             9034..11019
                     /locus_tag="PC399_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2591303.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            11024..11458
                     /locus_tag="PC399_000425"
     CDS             11024..11458
                     /locus_tag="PC399_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF2591304.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11487..12047
                     /gene="srlA"
                     /locus_tag="PC399_000426"
     CDS             11487..12047
                     /gene="srlA"
                     /locus_tag="PC399_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2591305.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12111..12479
                     /locus_tag="PC399_000427"
     CDS             12111..12479
                     /locus_tag="PC399_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF2591306.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12504..13070
                     /locus_tag="PC399_000428"
     CDS             12504..13070
                     /locus_tag="PC399_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2591307.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13111..13476
                     /locus_tag="PC399_000429"
     CDS             13111..13476
                     /locus_tag="PC399_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2591308.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13577..14350
                     /gene="srlD"
                     /locus_tag="PC399_000430"
     CDS             13577..14350
                     /gene="srlD"
                     /locus_tag="PC399_000430"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF2591309.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14699..17137
                     /locus_tag="PC399_000431"
     CDS             14699..17137
                     /locus_tag="PC399_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2591310.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDMALHKVNYVSDELDTTSNKVNYVSDELDMALHKVNGVSDELDMA
                     LHKVNCVSDELDMTSNKVNYVSDELDMTSNKVNYVLDELDITSNKTDYISNELYNLSN
                     KSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTVTLCDMFIP
                     CTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKR
                     TYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKNLG
                     ISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINQRMAKKVG
                     IEEKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQ
                     VTYNESMVEIIRQN"
     gene            17225..17560
                     /gene="spoIIAA"
                     /locus_tag="PC399_000432"
     CDS             17225..17560
                     /gene="spoIIAA"
                     /locus_tag="PC399_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF2591311.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17574..18002
                     /locus_tag="PC399_000433"
     CDS             17574..18002
                     /locus_tag="PC399_000433"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF2591312.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            18025..18789
                     /gene="sigF"
                     /locus_tag="PC399_000434"
     CDS             18025..18789
                     /gene="sigF"
                     /locus_tag="PC399_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF2591313.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            19069..19506
                     /gene="spoVAC"
                     /locus_tag="PC399_000435"
     CDS             19069..19506
                     /gene="spoVAC"
                     /locus_tag="PC399_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF2591314.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19673..20686
                     /gene="spoVAD"
                     /locus_tag="PC399_000436"
     CDS             19673..20686
                     /gene="spoVAD"
                     /locus_tag="PC399_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF2591315.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20700..21062
                     /gene="spoVAE"
                     /locus_tag="PC399_000437"
     CDS             20700..21062
                     /gene="spoVAE"
                     /locus_tag="PC399_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF2591316.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            21245..22261
                     /locus_tag="PC399_000438"
     CDS             21245..22261
                     /locus_tag="PC399_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2591317.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            22636..23478
                     /locus_tag="PC399_000439"
     CDS             22636..23478
                     /locus_tag="PC399_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2591318.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            23493..23957
                     /locus_tag="PC399_000440"
     CDS             23493..23957
                     /locus_tag="PC399_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591319.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            23944..25116
                     /locus_tag="PC399_000441"
     CDS             23944..25116
                     /locus_tag="PC399_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2591320.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            25160..26095
                     /locus_tag="PC399_000442"
     CDS             25160..26095
                     /locus_tag="PC399_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF2591321.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(26369..29062)
                     /locus_tag="PC399_000443"
     CDS             complement(26369..29062)
                     /locus_tag="PC399_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2591322.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFKENNEWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            29276..29974
                     /gene="yunB"
                     /locus_tag="PC399_000444"
     CDS             29276..29974
                     /gene="yunB"
                     /locus_tag="PC399_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF2591323.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(30190..31203)
                     /locus_tag="PC399_000445"
     CDS             complement(30190..31203)
                     /locus_tag="PC399_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2591324.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLFLLYFFSNNLI
                     YAQNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYS
                     DFANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTS
                     EFGAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENT
                     DFGIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKH
                     PESKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIK
                     WML"
     gene            31431..32315
                     /locus_tag="PC399_000446"
     CDS             31431..32315
                     /locus_tag="PC399_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2591325.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ANKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            32517..34067
                     /locus_tag="PC399_000447"
     CDS             32517..34067
                     /locus_tag="PC399_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2591326.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            34096..34743
                     /locus_tag="PC399_000448"
     CDS             34096..34743
                     /locus_tag="PC399_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF2591327.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(34885..35145)
                     /locus_tag="PC399_000449"
     CDS             complement(34885..35145)
                     /locus_tag="PC399_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF2591328.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            35362..36645
                     /gene="hflX"
                     /locus_tag="PC399_000450"
     CDS             35362..36645
                     /gene="hflX"
                     /locus_tag="PC399_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2591329.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            36653..37330
                     /locus_tag="PC399_000451"
     CDS             36653..37330
                     /locus_tag="PC399_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF2591330.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDKAIKSLE
                     NAKNLKK"
     gene            37416..37859
                     /locus_tag="PC399_000452"
     CDS             37416..37859
                     /locus_tag="PC399_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2591331.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            38164..38808
                     /locus_tag="PC399_000453"
     CDS             38164..38808
                     /locus_tag="PC399_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF2591332.1"
                     /translation="MSNYRTLHEFGMDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(38894..39271)
                     /locus_tag="PC399_000454"
     CDS             complement(38894..39271)
                     /locus_tag="PC399_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2591333.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(39271..39657)
                     /locus_tag="PC399_000455"
     CDS             complement(39271..39657)
                     /locus_tag="PC399_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2591334.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            39836..40591
                     /gene="nadE"
                     /locus_tag="PC399_000456"
     CDS             39836..40591
                     /gene="nadE"
                     /locus_tag="PC399_000456"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF2591335.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            40674..41393
                     /locus_tag="PC399_000457"
     CDS             40674..41393
                     /locus_tag="PC399_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2591336.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(41560..42564)
                     /locus_tag="PC399_000458"
     CDS             complement(41560..42564)
                     /locus_tag="PC399_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2591337.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            42830..43726
                     /locus_tag="PC399_000459"
     CDS             42830..43726
                     /locus_tag="PC399_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF2591338.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREMNLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            43864..45126
                     /locus_tag="PC399_000460"
     CDS             43864..45126
                     /locus_tag="PC399_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591339.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            45202..46395
                     /locus_tag="PC399_000461"
     CDS             45202..46395
                     /locus_tag="PC399_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2591340.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSIKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            47024..47818
                     /locus_tag="PC399_000462"
     CDS             47024..47818
                     /locus_tag="PC399_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2591341.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGVFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            47913..49253
                     /locus_tag="PC399_000463"
     CDS             47913..49253
                     /locus_tag="PC399_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2591342.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            49390..50622
                     /locus_tag="PC399_000464"
     CDS             49390..50622
                     /locus_tag="PC399_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2591343.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            50662..51804
                     /locus_tag="PC399_000465"
     CDS             50662..51804
                     /locus_tag="PC399_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2591344.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            51815..52609
                     /locus_tag="PC399_000466"
     CDS             51815..52609
                     /locus_tag="PC399_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2591345.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            52620..53642
                     /locus_tag="PC399_000467"
     CDS             52620..53642
                     /locus_tag="PC399_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2591346.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            53741..55117
                     /locus_tag="PC399_000468"
     CDS             53741..55117
                     /locus_tag="PC399_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2591347.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNRSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(55449..55793)
                     /locus_tag="PC399_000469"
     CDS             complement(55449..55793)
                     /locus_tag="PC399_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591348.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            56281..57291
                     /locus_tag="PC399_000470"
     CDS             56281..57291
                     /locus_tag="PC399_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2591349.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            57402..57890
                     /locus_tag="PC399_000471"
     CDS             57402..57890
                     /locus_tag="PC399_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2591350.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            58066..59994
                     /locus_tag="PC399_000472"
     CDS             58066..59994
                     /locus_tag="PC399_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2591351.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            60171..60581
                     /locus_tag="PC399_000473"
     CDS             60171..60581
                     /locus_tag="PC399_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF2591352.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            60603..60905
                     /locus_tag="PC399_000474"
     CDS             60603..60905
                     /locus_tag="PC399_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF2591353.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(61122..61814)
                     /locus_tag="PC399_000475"
     CDS             complement(61122..61814)
                     /locus_tag="PC399_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2591354.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(61840..63027)
                     /locus_tag="PC399_000476"
     CDS             complement(61840..63027)
                     /locus_tag="PC399_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2591355.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            63363..65240
                     /locus_tag="PC399_000477"
     CDS             63363..65240
                     /locus_tag="PC399_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2591356.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKVGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            65550..66956
                     /locus_tag="PC399_000478"
     CDS             65550..66956
                     /locus_tag="PC399_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2591357.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDIYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            66984..67832
                     /locus_tag="PC399_000479"
     CDS             66984..67832
                     /locus_tag="PC399_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2591358.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNEKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            68334..69026
                     /locus_tag="PC399_000480"
     CDS             68334..69026
                     /locus_tag="PC399_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591359.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            69023..69958
                     /locus_tag="PC399_000481"
     CDS             69023..69958
                     /locus_tag="PC399_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2591360.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            70106..71029
                     /locus_tag="PC399_000482"
     CDS             70106..71029
                     /locus_tag="PC399_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591361.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            71013..71783
                     /locus_tag="PC399_000483"
     CDS             71013..71783
                     /locus_tag="PC399_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591362.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TVLRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            71793..72575
                     /locus_tag="PC399_000484"
     CDS             71793..72575
                     /locus_tag="PC399_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591363.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            72952..73488
                     /locus_tag="PC399_000485"
     CDS             72952..73488
                     /locus_tag="PC399_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2591364.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            73520..73909
                     /locus_tag="PC399_000486"
     CDS             73520..73909
                     /locus_tag="PC399_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2591365.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            73946..74332
                     /locus_tag="PC399_000487"
     CDS             73946..74332
                     /locus_tag="PC399_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2591366.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            74459..75901
                     /locus_tag="PC399_000488"
     CDS             74459..75901
                     /locus_tag="PC399_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2591367.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            76198..77013
                     /locus_tag="PC399_000489"
     CDS             76198..77013
                     /locus_tag="PC399_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2591368.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        28990 a         9087 c        13916 g        25085 t
ORIGIN      
        1 acattctaat tttgtagtgt aatcgtattt tttattaatt cttacaaaat caatgaaaat
       61 aagtatatcc attttaaagt acataaattt aaaaatccag aaaaatatac ttaaattatg
      121 taagtaaaag aggggttgaa tcagttgaaa atgtaatatt agcaaatagt acatcaatcc
      181 tatataccct gtaagcaact ctattttcat gtactaaaaa tgtacctatt ttattaactg
      241 aaaagaataa attaaatgta agtacataag aagatagaaa aaattaaaaa caaaaaatat
      301 ttatataaat aaatataaaa aatgatataa tccctattaa aaaatttata gatgatgaag
      361 aaatatcaaa atcttatatt ataggaagag acacttcttt gccaaaatct atagaaaaac
      421 aataccaaat ccaaagagaa taggcggaga aaaaaataaa ataaattcaa gagtaataga
      481 aaagttttat agaataaaga actgataact ctttatataa cgaaaaatag aattttaaga
      541 gaaaatgatt taataactga tttaacatgg tgttttagca ggtaaaaata aatcaccagt
      601 aatgattata gaaaaattac tttatgaatc tcaaaaaact tagttaagag taagagattt
      661 gataagataa ctaaagttgg ggaaatgtgt ttgatgagac aaaaaattag taaaatagta
      721 taatctaaat aaggatattt tgttgattaa actacactaa tagtaatatt gtagggggaa
      781 aagatgagag aagaatcgaa ctctttaagt ttagaccata tagaaatttt ctttgaactt
      841 ttatctcaaa gtacagaaga ttatatattt ttttgggata taaataggaa taaatttaaa
      901 atttcttcag caatttttga tgaatttaac ttaagtaaag agatagaatc tgatttggtc
      961 aattgttgga gtaaaattgt ttatccagat gatgttcaaa tatggaaaga tgatatacaa
     1021 gaattactac atggaaaaaa aggtgagcac aatttagaat ataggttgat taataaatat
     1081 aaagaaatag tatggatttc ttgtagaggt aaagtttatg tgtcggatga cccaaaaact
     1141 atatttctag tcgggagaat aaagaatatt ggtgaaaaaa ataaatttga ttctatcaca
     1201 ggaacgtgga atagagaaca atttgaacat aaaatgaatt atcttataaa agagaaaatt
     1261 tataaaaatg gtgctatgtt tattatggat attgataatt ttaaaaatat aaatgaaaaa
     1321 tatggacatt cttatggtga caaagtgttg cgtgcaattg ctactgaagt actggaatat
     1381 cttccaaaag atgtaagatt gtataggtta gatggagatg aatttgcatt tttttaccct
     1441 atgtgtacta aagaaactat agagaaaata tatgaaaaga tacagatgta tacaaatact
     1501 cagcatgaaa ttgaatcaag taagtactat tgcacagtga ctgcaggcgt tgctatgtat
     1561 ccagaagatg gagataatta tttagattta tttaaacatg ctgatattgc attggatata
     1621 gcaaaaatta gtggtaaaaa tagaattaag tttttttcac aagagctcta tgaaaataaa
     1681 ctaaaggtaa tttcaatgca acaaaaacta agagagtgcg ttgaaaataa ttttaatgac
     1741 tttgagctgt tttttcaacc ccaggtaaat gctgttacaa aagaggtaat aggggcagag
     1801 gtacttttaa gatggcattc gagtacttat ggagaagtat ctcctgttga atttatacca
     1861 atactagaac agagtaattt aattatacca gttgggaagt ggattataaa agaagctgta
     1921 aaacagtgta aagaatggca taaaataaat ccagacttta aaatatctgt aaatgtatcg
     1981 tatattcagt taaaggaaga tttttttaga gattttatag tagaatgttt agtagagtat
     2041 caattaagac ctgaattttt aattttggaa ttaacagaga attgttggat accagatata
     2101 aatttactaa atgataaatt tattagttta aagggtatag gagtatatat tgcaatagat
     2161 gactttggta caggatattc atctttaaat taccttaaag aactgtctgt taatataata
     2221 aaaattgaaa gaagttttgt taagaatatt acatataata gttatgaata tacttttctt
     2281 gaatatatta ttaaattagc tcatattata aatttaaaag tttgtgttga gggaatagag
     2341 tcttatgaag agtatgatat agtaaaaagc ttaggggtag atattattca gggtttttta
     2401 tttggaagac ctgtaagtgc ctctgaattt tataaatttc agctatcaaa gtgatgtata
     2461 taaaatgaga tatatagtat ataaaaatag ggcaaagtgg aagttttgaa gtttattaaa
     2521 aatttacact ttaccctatt ttgttatgtc ttctataaag gattaatata ctacactaac
     2581 tattatatac tttcactcca aatttagaga ttatttcact agctttttgg acatcttcct
     2641 tagtggcatc aggaacatct tttagttcat aatcgatacc taaactttcc catttgtgta
     2701 ttcccataga atggtatggt agcaattcaa atctttctac atttttcaat gtagctacaa
     2761 attgacctag tttttctaaa ttatctatat catcagtaat gccaggaact aaaacatgtc
     2821 ttatccatac aggaatattt cttgattcta agtgtctagc aagttttaat gccttatcca
     2881 taccaacacc agtcaaatca atagattttt cctcaaccat atgttttaaa tcaagtaaaa
     2941 ccaaatcagt attatctaaa actgggtcta tagtttctat atctacaaat ccagatgtat
     3001 ccaagcaagt gtgtatatca ttttcttttg cttttttaaa taattcactt aaaaattctg
     3061 gttgaagagt ggattcacca ccagaggcag ttatacctcc accagaaaac ttcataaaag
     3121 aagtatactt aagagcatcc gttattattt catcaactgt gtattctttt cctgatttaa
     3181 tatcccatgt atccctgtta tgacagtatt tgcatctaag aggacacccc tgaaaaaata
     3241 atatatatct tattcctggt ccatctacag taccaaacgt ttctatagaa tgtatctttc
     3301 ccttaatcat agagttcacc tcacaatata ttttaataac cttatcaata gttataatat
     3361 ttctctaaaa aaggccatct tataccagaa ataattcaaa tataagatag ccttttattt
     3421 ttatttttta cacagtagta atcttagtta aagtataaaa atttaaagac taagccattt
     3481 taccgtggaa tgttctgttt ataacatcta attgttgctc tttagttaat tttatgaagt
     3541 taactgcata tccagatact cttatagtta attgtggata ttcttctgga tgttccatag
     3601 catcttctaa agtagctcta tcaaatacat ttacatttaa gtgatgagca ttttgagcga
     3661 agtatccatc catcattgca gaaagattgt ttattctttc agtcatatct tttcctaaag
     3721 cagcaggtac tatagagaaa gtattagata taccatcttg agcatgttca tatggtaatt
     3781 tagcaactga agataatgaa gctaaagcac cattagtatc tctaccatgc attgggttag
     3841 caccaggagc aaatggagca ccagctcttc taccatctgg tgtattacca gttttcttac
     3901 cataaacaac atttgaagtt attgtaagta tagattgagt aggataagaa tttctataag
     3961 ttttattctt tcttatttta ttcataaagc tttcaactaa ctctacagct atactatcaa
     4021 ctctatcatc attatttccg tattttggat aatctccttc tatttcaaaa tcaacagcta
     4081 ttccttgttc atttctaata gttttaacct tagcatgttt tatagcagat aatgagtctg
     4141 cacaaactga taatccagcc ataccacaag ccatagttct aaatatatct ctatcatgta
     4201 atgccatttc taatgcttca taagaatatt tatcatgcat ataatgtatt acatttaaag
     4261 tatttacata taatgttgct aaccaatcag tgaatggttc aaatttactc ataacttcat
     4321 cataatctaa gtactcagaa gttataggtt caaatcttgg tccaacttga acaccagatt
     4381 tctcatctac accaccattt atagcgtata ataaagtttt agctaagtta actctagctc
     4441 caaagaactg catttgttta ccaattctca ttgcagatac acaacaagct ataccatagt
     4501 catctcccca gtatgctctc attaaatcat cattttcata ctgtactgaa cttgtatcta
     4561 tagatacttt agaacagaaa tctttgaatc cttgaggtaa tttagttgac catagaactg
     4621 ttaagtttgg ttctggagaa ggccctaatg tatatagagt atttaacatt ctaaatgagt
     4681 ttttagtaac taatgttcta ccatctattc ccatacctcc tatacactca gttacccaag
     4741 ttgggtctcc agagaataag ttattgtaat caggagttct taggaactta accattctta
     4801 atttcataac aaaatggtcc atatactctt gtaattcttc ttctgttacg attccagctt
     4861 ttaaatctct ttcaaagtat atatctaaga aagtagatgt tctacctaaa gacattgcag
     4921 caccattttg gtctttaaca gcacctaaat aaccaaagta taaccattgt acagcttctt
     4981 ttgagttagt tgctggtttt gatatatcaa atccataaga ttcagccatt tttttaagtt
     5041 catttaaagc tgatatttgc tcagttattt cttctcttaa tctgataact tcctcatcca
     5101 tgcaagaaac ttctaatgat ttcttttgct catttttatc ttctattaaa gcatcaacac
     5161 catatagagc aactcttctg tagtcaccaa ttattctacc tctaccataa gcatctggaa
     5221 gacctgttat tataccagat tttctagcag ctctcatttc tggagtataa acatcaaaaa
     5281 ctccttggtt gtgtgttttt ctgtatttag taaatatatc ttttatttct gggtctaatt
     5341 catatccaaa agcttcgcat gaattttcaa ccatctttat accaccatat ggcatgacag
     5401 ctctttttaa aggggcatcc gtttgtaacc caactattgt ttctaattct ttatctaaat
     5461 atcctggagc atatgcagct attccagaaa cagtttttgt atcaacatct aaagtaccac
     5521 cattttctct ttctttttta aataggacca ttgcttcgtc ccataatttt ttagtatttt
     5581 cagtagcacc agctaagaaa gagtcatttc cctcatatgg agaatagttt aattgtataa
     5641 actctctaac atttatttct ttagtccatc ttcctgtttt aaatccttgc catgcattca
     5701 ttaagtattt cctccttaga tttaaatgat aaataatact attaaaatta atttttatat
     5761 acttaattta ttttatatat ttttttaaaa atatattttc taatataata atatcatatt
     5821 attcgtatcc tgtatacata aaaaataaaa aattgtcgct ttaaagtata aaacagatta
     5881 tatgttaata atgtggtgaa attataccca tatataaata aaataaaaca aataaatagc
     5941 taattaagga gaaaatattt tttatttttt gtttaggata aataaatttt attatggaaa
     6001 aaagaaaaag catacatctt cacaaattga atatttattt aataggggga ggtgatagat
     6061 tgtttatcac agtggtatta tttttagtag gtttcctatt aattactaaa ggcgcagata
     6121 tctttataaa ttgcactgta gagataggta aaaaaacgaa tatatcagaa attatacttg
     6181 gagctactat agttagtttt gcaacaacat tacctgaatt tacagtgtct ctacttgctt
     6241 ctattgatgg tcatactact atgagtttag gaaatgcagt tggttctata atatgtaata
     6301 caggattagc attagggttg gttgttttta taagtccatt taatgtagat aaaaaaatgt
     6361 ttttttctaa gtctttacta ttaatagtat ccgttatagt tttaatttta ttgagtttag
     6421 atggagttat aacaagaggt gattcattat tacttataat aatattaatc ttttatatgg
     6481 ttaataatta tagaagtgtc gttggaaaat ctgatactaa aaatagaaat attaaaaata
     6541 acactattaa agacatatct actaaaaaag ggaaaaatta tagaggtttt tccgtattag
     6601 aaattgtaaa aatactatta ctttttgcta caggattgat aatgatgata ataggttcac
     6661 aaatccttat agagagtgga gtaataatag ctagtttttt aaatatacct caaggtatag
     6721 taagtttaac tataatagca cttggaactt ctctgccaga gatagtttcg tcgattactg
     6781 ctataagaaa aaatcatcat gaaatatcag taggaaatat tttgggtgca aacatattga
     6841 atatagtatc agtaatagct gtatcagcaa taccaaataa cataccaata ctatctcaaa
     6901 atagacaatt ggatatacca ttcatgatat tattattatt aatagtaata ataccaacac
     6961 ttaaatctaa tagacttagt agaatacaag gtatattgat gttattcaca tattttctgt
     7021 acatatcaat tttgtatttc atgtatatta tttaactata ctgtttaaaa tattaattgc
     7081 agaatatcct aaaaaagtat ataatggata ggttaagatt aattgataac ggagaggaat
     7141 aatatatgaa tataacaaag tttgaagatt taccaataag tgagggaata aaaaaagcta
     7201 tagctgaaat ggggtttgaa gaaccatctc ctatacaagc acagtctata cctgcgattt
     7261 tgtcaggtaa agacgttata ggacaagctc aaactggtac gggaaaaaca gctgctttta
     7321 gtatccctat actagaaaca atagacccaa ataatagaag tttacaagct gtagtgcttt
     7381 gtccaacaag agaactggca atacaagtat caacagaaat aagaaagtta gctaagtatt
     7441 cgcacggaat aaagacatta cctatatatg gtggtcagcc aatagataga caaataaaat
     7501 ctctaaagag tggtgtgcaa gttgtaatag gtacaccagg acgtacaata gaccatatca
     7561 atcgtaaaac attaaagatg gataatgtaa aaatgattat attagatgaa gcagatgaaa
     7621 tgctagatat gggatttaga gaagatatag aaatgatttt aagtaagatt ccagaagaaa
     7681 gacaaacaac tttcttttca gctactatgc caagaggaat tttggaatta actaagagat
     7741 atcaaaaaga cccagagcat ataaaagttg ttagaaaaga acttacagtt tcaaatacaa
     7801 agcaatacta tatagaaact aggtcatcaa ataaattaga agtattatgt agattagtag
     7861 atgtatatga ccctaaacta tctgttgttt tctgtaatac taagagaaaa gcagacgaat
     7921 tggtaggaga tttacaagca agaggatatt ttgcagatgc attacatggt gacttaaaac
     7981 agactcaaag agatatagta atggataagt ttagaaatgg tacaatagat atacttgttg
     8041 ctactgatgt tgcagctaga ggtatagatg ttgatgatgt tgaatgtgta ttcaactatg
     8101 acttaccaca agatgaagag tattacgttc atagaattgg tagaactggt cgtgctggta
     8161 gagaaggtat gtcatttact tttgtatttg gaaaagaaat gagaaagatg aaagatatag
     8221 aaagatatac taaatctaaa ctaataaagc ataatatacc aacaataact gatgttgaag
     8281 agaaaaaagt aggtactttc tttgcacaag ttaaacaaac tatagaagaa ggtcacttaa
     8341 ctaaacaatt acaatggtta gaaggtttct gtaatgatga agattatgca atggttgata
     8401 tagctgctgc acttgtaaaa ctatctctag gtgaagaaat gaaagaagaa ataatagaag
     8461 aaaaacctag aagagagcgt ggagatagaa aaggtggtac tggagctaaa gatggtatga
     8521 ttagattatt tatcaatatt ggtagaaatc aaagagtaca agcaaaagat atagttggtg
     8581 ccatagctgg agaagttgga ataccaggta aagtagtagg tactatagat atatatgata
     8641 agtatacttt tgttgaaata cctaaaaaag atgcaaaaac agtaattgaa aagatgaaag
     8701 atattaagat aaaaggtaat aagattaata tagaaaaagc aaacaagaag aaaaaataac
     8761 gtatagtata caaaactata attagaaaat atataaaata atatattgga tatttaaaaa
     8821 gcctgatgtg ttttcaaaac agatagtaga taatctgtaa gagttaacag cataaggctt
     8881 ttcttgtttt atatgatgtt ataattttcc agattgttgt gaaaaatgta tattagttcc
     8941 tttttttcaa atctgatttg aaaaagagaa tattaattat ctatatagtt tagattataa
     9001 taaaaaatgt aagataacaa aggggagaaa aagatgtcaa tgacttatat ggaccctagg
     9061 gtttttttga ttttagaaat ggtgttagaa gaagacacaa atactctaga acaattttca
     9121 aatgttttgg gtgttagtac taggactatc agaaactata taaaccagat taatcgagaa
     9181 ttaaaaggtg gtattgctaa aatagttaaa aatgaaaaag gtacatatag tataagaatt
     9241 gaggatgaaa gtaaattaat tgagattgta aattttaata gaggaaagct atcaaatttt
     9301 ataaatttaa attcacctga tgaaagaata aattatgttc ttgatatttt aactatgacc
     9361 aacaatccaa ttaccataga tgatttggct gaagagatat gtataggaag aacaacatta
     9421 ataaaggatt taaaaaaagc agacagaatt ttagctgaat atgatttaga attaaaaagg
     9481 aagccaaata taggtatgat tttagttggt gaagaaatgg atataagatt attaatcctt
     9541 gatagattgt atgaaaatta tattgatgtc ttggaaaacg ttaatcagat aaatttagtt
     9601 aaagattatg atattgaatg tcttagagat gaattaaaaa agttatttaa aaaagaagaa
     9661 ctctacataa cagaacaggt cttgagagat gttgaaagat atataatcat gtctgtctta
     9721 agaaatctaa atgggtacaa atttgaaaag atagataaga gatttgaagt gataagatgt
     9781 tcagatgaat atactttagg attaaagctc aaagctttat tggaaaagat atttaatata
     9841 gttttaaatg aaaaagaaac catatttttg acaatgccat taataggtag aaaagctcct
     9901 agtacaaaat tagcacttag cagtataaag ataaatgata gcgtaaagga agttgtagat
     9961 gaagtaacta gttttttatt agagacctca gggattgatt ttaaagaaga taaaaagctt
    10021 atagaaaatt tggaatatca cttgtatttt gcacttaata gaatgagatt caacataagg
    10081 gtaaaaaatc ctcttttaca agaaataaag gaaagatata catttccata tagtgttgca
    10141 aagatagctg caatgataat tgaggaaaag tttgacttga aaatatgtga ccacgaaata
    10201 ggatatatag cattacattt tggaagttat tttgaaaaaa attcaagaaa agttctaaat
    10261 gtaaataggg tagctgttgt ttgtggaaca ggtcttggga ctgcacaact tattagaata
    10321 aagataggaa aaatacttgg agaaagtgta gaagtaaata ctttttctga tatagaagct
    10381 aaagctaatt tagatttatt aaagtattat gatttagtat ttacaacagt agatttcaaa
    10441 acagacttag atgttccaat ttttagagta aatgcgttat ttaatgaaga aagtataaag
    10501 aaagagatag aaaatgcaat acttttgaaa aatagtataa atcttaagtc tataaaaaag
    10561 attgaaaaaa atatcccttt tataggtaaa ttaattgaag aggataagtt ctttatattg
    10621 aataagagta cgtttatgga aaatttagaa gatatgttag aaaaattagt tcataaagga
    10681 attattgata tagagttcaa aaaaaaggta ttaaaaagag aaaagaaatc ttcaacagca
    10741 ttagataatt atatagcatt gcctcactca gttaatacta atggggaaaa tctttatctg
    10801 gcatttggaa tattagaaaa accagtgatt tgggatacaa aggaaattag aataataata
    10861 ttgatgataa ttccagataa gaatgtagat agcacagacc tgataataaa aagttatgaa
    10921 gaagtacttg aagtagggag agataaaaag atggtagaag aacttgctaa ggtaaaatca
    10981 tttgatgaat tttataaaat aattaccaag aggaggtaat aatatggacc cagtaatttt
    11041 tattatagca tcacttatta cagtgtattt attaaatatt tttcttggat acttacagct
    11101 taaagatttt aataaaaatt atattgaact aaaaagaaaa ggaagagtgg caattggcag
    11161 aaaaaaagga agaatttcat caggtacaat tgtgttgata cttataaatg atgatggtgt
    11221 gattgtcgag actagaaaaa tgcaaggtgt tacagtattg gcaagagtaa agtcatttga
    11281 aggtttggta ggaaagagct tagagtatat agaaaaatca gatttaactg tttacaataa
    11341 actacttaaa aaagcaatac tagatgcagt caaacagtac ataacatttg agaaaaataa
    11401 atgtgaagat acaaataatc tgagtactga tgaagagtgt aggatggaag tagtttaaaa
    11461 aaatatattt ataaggagga gttaatatga cgcaattttt tcaagtatta gcaagtggtg
    11521 cagatagttt tatgaattta tttagggcag gtgggcaaca atttgtaggt tttgtaacag
    11581 atatagtacc acttttagta gcattattag ttacaatgaa tgcaattatc aactttattg
    11641 gtacagatag agtcgagaga cttgcaaaaa aatgttctgc aaatatattt actagatatt
    11701 tgatattacc agtccttggt acatttgtat ttgcaaaccc aatgacacta tcattaggaa
    11761 gattcttgcc agaaaagttt aaacctagtt actttgcagc agcaagcttt tcatgccata
    11821 caatgaatgg attattccca catatcaatc caggtgaact atttatatat ctaggaattg
    11881 caaatggtat aacaactttg ggattctcaa cagcagattt agcagttaga tatttgttag
    11941 tgggtatagt tgcaaacttt attaaaggtg tagttactga ctatacaaca aaatttgtag
    12001 agaaacaaca aggaatagca ttaaattcag atataaagat tggataatta ctttaaggta
    12061 taaataaatt tttcaaataa aaagatagtg tatagaggag gataaattat atggcattta
    12121 acaaagttaa aatagtaaaa ggttctggag gatggggtgg accattgata atagaaccaa
    12181 cagaaaagaa gaataaggta gtatatgtga ctgggggagc aaagccagaa actgcagtaa
    12241 aaatagctga acttacagga tgtgagttaa tagatggatt ttcacagggt gttaaagatg
    12301 atgaaatcgc atgtgtaata ataaattgtg gtggaacatt gagatgtggt atatatcctc
    12361 aaaagaaaat accgactata aatataatga aaacaggaaa aagtggacca ttagcaatgt
    12421 ttataaaaga agatatatat gtttcagggg taaaaccaac aaatgtagag cagttataat
    12481 tgaggtagta ggagggaaga aatatggctt atgatactaa aaagaagata agtgagcaga
    12541 gtcaaggaat tgttgcaaaa ataggtatag cactaggaaa tgtgacaagt actctttttc
    12601 aagctggtag ggatactata gatacattaa tacatactat actgcctttt atggctttcg
    12661 ttgcagtcct aataggtata ataaatcaat cgggtatagg aaattgggta gccaatttac
    12721 ttactccatt aatcggaaat gttggaggtt tagttgtctt atcaataata tgttcatttc
    12781 cgttgttgtc tccgtttctt ggaccaggag ctgttatagc tcagatagtg ggggtattgg
    12841 ttggtacaga aataggaaaa ggaaatattc caccaagttt agcattacca gcattgtttg
    12901 caataaactc acaagcagct tgtgacttta taccagtagg tcttggtctt gcagaagctg
    12961 aacctgatac agttcaagta ggagttccgt cagttttata ttctagattt ttaacaggtg
    13021 caccaactgt tctattagca tggttagcaa gctttgggtt atataaatag aagaatactt
    13081 tataaataat tagagagagg ttcagttgat atggaattaa tatacagtac tcaaataaat
    13141 aaaataggag ataatgcaaa tgaattttta caacataata tgtttgtaac tttcaaagat
    13201 aatgctcctc aagagttaga aaattactgt tatatacatt ctgaaaataa tttagtaaaa
    13261 gaaatactta cggatgatgt gctatcaata aataatgttg attacaaaat tacttctgtt
    13321 ggtgagttag taaaccaaaa tctttcagaa ttaggtcata taacatttaa gtttacaggt
    13381 gaaaaagatg gagcaattgg aggaacctta tatcttgaag aaaaagaaat agcacctatt
    13441 ggcgtaggta ctatactaaa aataattaga aaataaaatt taaaacgaat ctaatcatat
    13501 tataagtaaa ttatagataa aagtcaattt aactaatcaa gttaactttt aatgtgataa
    13561 aaaaggggaa atgaaaatga gaaaagtggc tttagtcgtt ggaggaggaa gaagtttagg
    13621 tgaatattta tctaaacatt tagctaaagt aggttatgat gttgctattg cagatttaaa
    13681 tgaagacaac gcaaaaaaag tagctgaaga aataaaaact gaatttggtt gtaaatctgt
    13741 aggatatagt tgtaatgcca tagatgaaag acaggttata agtactgtca aagctattga
    13801 aaatgatttt gatagaatag atttattagt gtacaatgca ggagtggctg taagtaaaaa
    13861 aataactgat tttggataca aagattttaa atttattgta gacgtaaatt taaatgggta
    13921 ctttctattt gcaagagaag tatctaaagt catgatagca aaaggtattg agggtagaat
    13981 aatacagata aattcaaaat caggtagagt gggaagcaag cataattcag gatattcagc
    14041 aggtaaattt gctggagtag gacttactca aagtttagcg ctagatttgg cagaacataa
    14101 gataactgta aatgcactga tgctcggtaa tttacttgat tctgatatgt ttgaaagttt
    14161 aattcctcaa tatgcagtaa aattgggaat acctgaatca gaagtcaagc aagtttatat
    14221 agataaagta cctttaaaaa gagggtgtag atttatagat gttgcaaata tgcttacatt
    14281 ttatgcttca aaggaagctg aatactgcac tggtcaatct ataaatatta ctggtggaca
    14341 agttatgtaa ggtttttatg gagtgtctca agatgaactc tttagttcat gaggcacttt
    14401 tttgtataaa attaaatata ttttcagcat ttaaacaatg aaaaagaggt ttatctttat
    14461 tttgagatag cctcattttt gtaattaaaa aacatagtgt ttaagtaaac atataagatt
    14521 gttagttttt taaaagagaa ttttttaata atattatatt tatgaagtat ttttaaactt
    14581 aatttgataa aatatagcta tgttttatat actgtagttt tgaacataga aagtttaata
    14641 aattatagta tatatgttaa tttttaataa ggtaagaaag tagaaagaag gatattttat
    14701 ggatataata aaaaaattaa gtcatgggtg tacattagat tgtcatgact gttgtaaatt
    14761 caatgtttat actaaaggaa ataatgtagt aaaaattgaa ggagataaaa atcatcctta
    14821 tacaaaaggt tttatatgca aaaaagggat ggcccattta gatagattaa atcataaaga
    14881 tagaataaag actcctatgc ttaaagttga tggagtatgg aaagagattt cttttgataa
    14941 agctatagaa ataatggcag aaaagcttac atactataaa gaaaagtata catcaaagtc
    15001 tgtcatgcat tatgaccaat atggaagtgg gtctgtgcta aaatacatag gagatatatt
    15061 ttttaatttt tatggaggtg ttagcagaca taagggtggt ccctgttgga gtgcagggat
    15121 gcatgctcaa aaatatgatt ttggagtagc taagagtcat gctatagaag atatgttaaa
    15181 tagtaaaagt atatttgttt ggggaaaaaa tccagcatat actacaattc atactatgca
    15241 aataattaaa aaagctaaag aaaaagggat aaagatagta gtgattgacc ccatatatac
    15301 aaaaacagct caaatagcag ataagtatgt acaagtaaat cctggaacag atggagcttt
    15361 agctatagct atggcaaaaa ttatagtgga agataaatta tatgatgaag aatatataaa
    15421 ctcgtatgtt attggttttg aagaatataa aaaatattta tcttcattgg aattaagttt
    15481 tttaattgat gaatgtggtg ttaaagagaa tgatataaga gagttagtgg atttatatac
    15541 taacaaatat tcaagtataa atgttggata tgggcttcaa aaatataaaa atggtggaaa
    15601 cacaataaga gcaatagatg ctttaggtgc tataactggt caaataggat ttagtggtgg
    15661 aggggtaaac tatgctaata aagtttatcc gagtgttata aattctgacc cttacaatag
    15721 ccaatcatat ggtgaagata gggagtttta tgtaagtaat atcagtaaat ttatagaaga
    15781 atctttaaaa aatacatcaa ataaagtaaa ttatgcatca gatgaacttg atatggcatt
    15841 acataaagta aattacatat cagatgaact tgatacaaca tcaaataaag taaattatgt
    15901 atcagatgaa cttgatatgg cattacataa agtaaattat gtgtcagatg aacttgatac
    15961 aacatcaaat aaagtaaatt atgtatcaga tgaacttgat atggcattac ataaagtaaa
    16021 tggtgtatca gatgaacttg atatggcatt acataaagta aattgtgtat cagatgaact
    16081 tgatatgaca tcaaataaag taaattatgt atcagatgaa cttgatatga catcaaataa
    16141 agtaaattat gtattagatg aacttgatat aacatcaaat aaaacagatt atatatcaaa
    16201 tgaattgtat aatttatcta ataagtcaat caaagataat attcctataa agatggctgt
    16261 gataactaaa agtaatatgc tgaatcaact gccagattta gtagaattgg agagggtgtt
    16321 ttcaaaaatt gaatttaaag tatgttttga tatgtttata actgatacag ttactttatg
    16381 tgacatgttt ataccgtgta caaatacact ggagagtgaa gatataattt atagctcaat
    16441 gaccaatcca tatataacct ataatgaaag agctgtaaag ccagcacata aattgatgga
    16501 tgaatattac ttctttatgg agctagctaa aaagatggga ttaaatgatt atccttttgt
    16561 agaaaagaga acttatttag aaaaagtgat tgaaccactt aagcgttttg acaaaaatct
    16621 ggatatagaa aaacttaaaa acaattactt tactatacat aaccctgttg cttgggaaga
    16681 taagaagttt gagactccat ctggaaaata cgagctttat tcagaaagta taaaaaactt
    16741 gggaataagt ccaacacctg tatatataag caataaatat aaagaaatag aagataaaaa
    16801 tatttctttt agattattaa ctaatcatca tgcagatact ttatttagtc agcattttat
    16861 ggacaaaaaa tctatagctc aagcatatat aaatcagaga atggctaaaa aagtaggtat
    16921 tgaggagaaa gatattgtga tattgaggtc aaaaaaagca aaaataaatg tacaaataaa
    16981 tatagatgat ggagtaggta attatatagt aaaaatgtat gttggttggt ggaagaaaca
    17041 tggaaatcct aatagtttaa cagacactgg tatatcagat tttgggggtc aggtaacata
    17101 taatgaaagt atggtagaaa taatacgaca aaactagtta aatttcattg aaggaataaa
    17161 aatataatta tagaattgat taaaagttac tatttttaca aaactattga aggagggatt
    17221 tttgatggta aattattcct tagaacataa gaatctatac atagaattta tgtgctcaga
    17281 actagaccac catgtagcca atgaaataag ggaagaaata gataacttat taagtgtaaa
    17341 tcaagtaaaa aatgttgtat ttaattttga aaatattaat tttatggatt cttcaggtat
    17401 aggcgtcatc ataggtagat ataaaaagat ttctaatgaa gggggacgag tttcagtaat
    17461 taatataagt tcacgagtta agaagatttt tgatttatct gggttaaata aaattattgg
    17521 catttatgat acgtatgaag aagctctaag ttctttgtag ggaggagtat gtgatgaata
    17581 atattatgga agttaaattt tcggcaatat ctgaaaatga aagtttagca agagttatag
    17641 tagcatcctt tgcagctaag ttagacccaa cagtagatga gttagtagat ataaaaactg
    17701 ctgtctctga ggctgttacg aatgctataa tacatggata tgaagaagac agttctaagt
    17761 ttgttttctt aagatgtgag atagaaggaa atactataaa gattatagtt gaagatgaag
    17821 ggtatggaat agaaaatgtg gagaaggcaa tggaaccact ttatacatca aaaccagaac
    17881 ttgatagatc aggaatggga ttcacagtaa tgaaaagctt tatggacgat gtagaggtaa
    17941 gttctgtaaa agataatggg actagaattg aaatgactaa aaaaataaat gtgcctaaat
    18001 aaagtccaaa agtgaggttt tattatggaa gtaactgttg ccagagaaga aaaaaaacct
    18061 cttcttagtc atgaagagac tctagaactt attgaaaaag ttcaaaatgg agatgaagag
    18121 gcaaaagaaa tattaatatc aagcaattta ggtctagtta ggagcgttgt atctagattt
    18181 ttaaatatag gatatgatag agaagattta tttcagttag gttctatagg cttaataaaa
    18241 tcaatatata aatttgaccc aaaatttaat gtaaaattct ctacttatgc agtaccaatg
    18301 attttaggtg aaataaagag atacttaaga gatgatggta tgataaaagt atcaaggtcg
    18361 ttaaagcaaa tagctgtaaa ggcaaagatg gaaagtgaag ctttaactaa aaaattaggt
    18421 agagagccga gtatagaaga attagctaaa gcaatagatg tagaaaaaga agatttagta
    18481 atggctatgg aagctaattt caatgttgag tatttgcaag gagttattca tgaagaggaa
    18541 ggttctccaa tatgccttat tgataaaata agcatgaaag gagaaagtga agaagaaaaa
    18601 gtagtggata acatattgtt aaaagacata cttggaagac tggataagag agagcgtcaa
    18661 ataatagttc tcagatactt tgaagacatg actcaaagtg aaataggaga gatgctcaat
    18721 atatcacaag ttcaagtatc taggatagag aagaaagtat tgtctaagtt aaaagaatat
    18781 atatcgtgat aatacttatg atatgtagaa taacaaaata taataaatat attactaaaa
    18841 taaaatgtaa taacttttta tgttaagttt ttaaattatt aaaagatatt aaaataataa
    18901 aaagcattaa aaaagtaaaa atataaaaac aaggtgtctt tatggaaaaa tagatttttt
    18961 ctttattagg catctttttt attttttgac caaactttag atagtcaaaa taaataatag
    19021 gtttattttt ttaaaattaa caaataatac tttagagagg tgtttagtat ggataaaaat
    19081 tataaaaaat atgtagacca aataagccca aaacctacat atttaaaaaa ctatacactt
    19141 gcatttattg taggtggaat tatttgtatg atagggcaag caataaatga tttgtatatg
    19201 aaagttggag gtcttgataa actaggtgct agttctgcaa catcaataac attaatattt
    19261 ataggggcat ttttaactgg attaggagtt tacgacctaa taggaaaaag agctggagct
    19321 ggttctataa ttccaataac aggttttgct aactcaatag tatctccagc aatggagtat
    19381 aaaagagaag gctatgtatt aggtgtaggt gcaaacttat ttaaaattgc aggaccagtt
    19441 ttagtttatg gtataggttc ttctatttta tgtggaatta tttattatat ttttaagatg
    19501 ttttagatga tttttattta atttaaattt tattaattat gaagaaggta taaaatctga
    19561 aaatagctac taaagtaact aattgaaaaa taattgtgca gatttaatta aaatttttaa
    19621 ttaatattaa aaatttgaat agtaaaatgc aaaattaaaa aggtgtgaaa atatgaaaaa
    19681 taaaagaatt ggaaaaagaa cagtcaagtt agaaaataaa ccaaccataa tatcaactgg
    19741 aactatagta ggtcctaagg aaggtgaagg accactaaag gactattttg atatgattat
    19801 gactgacgat ttatatggtg aaaagacttg ggaattagct gaaagtaaaa tggtagagac
    19861 tgcatctcaa caagctatcc aaaaggcagg taaaaaattg tcagatgtaa attatatgct
    19921 aggtggcgat ttaataaatc aaatagttcc agcatcattt gcagcaagag aactggctat
    19981 tccattttta ggtatatatg gagcttgttc tacaatggca gaaggtcttt gtataggttc
    20041 tatgcttata gatggtggtt ttgctgattt agtactgtca ggtacatcaa gtcactattg
    20101 tacagcagaa agacaattta gattcccact tgaacttgga aatcaaaaac caatgacagc
    20161 ccagtggaca gttacaggag caggaagtgt attattagct cctaatggag acggtccaaa
    20221 agtaaagtat gttactgttg gtaaagtaat agatgagggt attgatgatg gtaataatat
    20281 gggaccagct atggctccag cagcaataga tactatctac tcttactttg aagatactaa
    20341 tgatgaccca aatagctttg atataattgc tactggagat ttaggtactc ttggaaaaca
    20401 gatagcagaa gactttctaa aagaaaaagg tgtagatata tcaaaagttt atactgactg
    20461 tggtatagag atgtttaacc ttaaagaaca agatgtccac tgtggtggaa gtggatgtgg
    20521 gtgttcagcc acagtgtttg caggatatat atatgataag ttgagaaaaa aagaatttaa
    20581 caaagtaatg cttgtatcaa caggagcact actttcaccg acaagtacac tacaaaagca
    20641 gacaatacca tgtgttgcac atgcagtagt aatagtaaat gagtaggaag tgatataata
    20701 tgttaatgga ctatgtaaga gtatttattg taggtggaat tatatgttta atagcccaag
    20761 taatgatgga ttatttcaaa atgcaaactc catatgttat ggtaacgtat gtaacaactg
    20821 gtgtagtttt aacttttcta ggactttatg agccattagt taagtttgca gcagcaggtg
    20881 caacagttcc tataatagga tttggatatt cacttgctac aggagtaata aaatcaattc
    20941 aaagtgatgg ttttttaggt atttttttag gaggtacaac tgctacagct ggtggaatag
    21001 cagcagctat attctttgga tatattatgt cagtagtatt tactccaaaa gcaaaaccat
    21061 aaatctttat taaaagaata ataagttttt gaaataataa atttttataa agcaggtgga
    21121 tatcttttag attccacctg ttttttatat aaagaagcgt ttacataatt gaagttgaca
    21181 attatttaca acatagtgta gtattatatt atcaaaataa aatgaagtga agtaggggat
    21241 aaagatggta actattaaag atattgcaag gaatctaggt atcagttatt caactgtttc
    21301 taggtgctta aacaataatc ctaatgtatc tgaaaaaaca aaaaagaaag tggtagaaga
    21361 ggcaaataga ctagggttcc attttaatgt aaatgcaaga aatttagcaa aaaaagaaac
    21421 taacaggata ggggtaatct tttcaaataa ttttaatcat caagatacta gaaagttttt
    21481 tagtgatata atggatagtt ctataaattc tattgagact aataaatacg attttataat
    21541 acagcctaat aataacatta gtggtgatag taatgtatat aaaatggtaa atggacaaat
    21601 ggttgacgga ttagtaattg tgtctcaatc aataaaaaaa gaagagtatg attttttaaa
    21661 agacaataat tttccacatg tatttatata ttttaaacca tcttttatag gagaagtaga
    21721 taatttcttt tgggacgata acgtgtatgg aggttatata gctaccaagc atttaattga
    21781 ccatggtcat aaagatataa taacagtaac ttcagatgat aagagttcaa aaatgcatga
    21841 agatagaacc aagggatatt tgaatgctat ggaagaagca aatttaaaga cagaagtcat
    21901 taaatctaaa atggattttg aatctcaagt tgaattttta gaaaaataca ttgataaaat
    21961 aaaaaaagct tcagctatat ttgtacaaca agatgtacca gcgatttcta taatacaaga
    22021 gttaaaaact acttatggta taaatgtacc agaagatatt tccataatag ggtataataa
    22081 tattgaatta atatcatatt ttaggtcgca tttatcaaca atagatgacc caagagagca
    22141 agttataaaa aatggagtag atagtttagt aaatataata aataaaaaga gtacagaaca
    22201 cattccgaga aaattatatc caaggttaat aatacgaaat agcgttaaaa gaataaagta
    22261 gtaagctttt aagtatcaaa cgtttattaa aaatattata gattatgtga agttttttaa
    22321 caaataatat aaataaaaat taaaaggagt aggtgtatat aagtacattg tactccttta
    22381 tttattgaga tagtttttat aatatgaatg aataatttta ttcaattatt tgaaaatccg
    22441 ttattttaac aatataaatt aataaattat ttgtatttta tatcatgaat ctataatatt
    22501 tttttgattc ataactaaaa gtatataaaa atgatgaata aatatatatt atacattatc
    22561 aatgataggt tatataataa aatcactaac aattatatta taagaaaatt ataaaaatta
    22621 caaggaggta tagaaatgaa agaaaaagca gggattagaa atgccataat agcagccttt
    22681 ataatcacaa tattggcaga attaattgga cctatttcat ttaaagttat gggaattaat
    22741 gtaactttac ttagtatact atgggctata ttcataggaa tggctgtttc tcctcattta
    22801 ttagggagag ttataccagc cttaaaaaag tttatagggg ataatgaaat aaatgtatct
    22861 ccatatttat tatctttgac actatatcca ttagggataa tgtttggaat taatgcaggg
    22921 cctaaagttg gtatattact tcaagcagga ccagcacttt tattacaaga atttggtcat
    22981 atgggtacaa tgttgatagc attacctgtt ggagtagcat taggtcttgg aagaagtgca
    23041 ttaggtggta ctttttcatt atgtcgtgac acagcactag gaattattgg agataagtat
    23101 ggattaaact caccagaagg tataggaaca ctaggaactt acataagtgg tagtatattt
    23161 ggaacattat tattttcatt tttagcacca gtgggtgtac ttttaggatt tcatccatat
    23221 gctttagcaa tggcatcagg aatgggtagt ggttcaatga tgggagctgc aacagcatca
    23281 ttaatgaata cggttccaca aatgtcagag cagattttag catattcagc tactagtgga
    23341 ttgattacag ctgttactgg aatatatata gaattattct tatctttacc agtagccaac
    23401 tactattata gcaaagtagc tccagttatt gaaagattta gaggaagaaa aacaagaaga
    23461 acaagtaata aattgtaaag gaggatatat atatggaaaa aacttttaag actattatgg
    23521 agcagataaa aatagtattg tttatatata ttattatttg ctttggacaa atgatagcac
    23581 ttaagactca ggctcaagat ttgttgattg gaagtgtatc tggatttttg ttggtgataa
    23641 ttgctattat aataaagaat tttgttaaaa aacctaattt accaggtttt gcatgggcta
    23701 cattagttgc atttgtttta tcgcttccaa tatctccagt gggggatatc atagcaacaa
    23761 atgtagggaa aatcaatttt atgtctacag taacaccatt attagctttt gcaggaatct
    23821 ctgtaggtaa ccaattagat atattaaaga ctttgagttg gaagttagta gttatatcat
    23881 ttatagttat gacatctact tactttggtt ctgcttttat atctcaaaca gttttaagat
    23941 taaatggaat gctttaagga ggtagtaaat atgaatgaat taaaagcaaa aattaaagag
    24001 gaaataaagt tattatctga agagaaaaag acatcatttg aaaaagtttc tgactattta
    24061 ttttcaaatc cagaattagc atttgaggag tataagtcac aaaaagcatt atgtgatttg
    24121 ttagaagaaa atgaatttaa tgtaactaaa ggtgtaggag gattagaaac atcttttgaa
    24181 gctgtgtatt caaatggaac taatgggaaa acagttgctt ttttagctga atatgatgca
    24241 cttccaggaa tgggacatgc atgtgggcac aatatcatag ggacaagttc tgttggtgca
    24301 ggtatcatat taaaagaaat catgaaaaaa cataatatag aagggactgt aaaggttttt
    24361 ggaacacctg cagaagaaag agtaggtgga aaaataacta tgattaaaga aggtgtattt
    24421 aataatgtag atgctgcatt aattttacat ccaagtgatg cttcaatgcc tgatgatata
    24481 tcatttgctc aagttaattt aaaatttgat tttactggta aggcatcaca tgctgctgct
    24541 tttccttggg aagggaaaag tgccttaagt ggagtaatag cactatttaa cagtgtaaat
    24601 agcatgagat tacatcttaa agattatgca agagtacatg gaattataac tgatggaggt
    24661 agcatacata atataattcc tgaaaaatct acagctatat ttaatgtaag agctttaagc
    24721 atagaatatt taaatgagat ttgtgaaatg cttaaaaatt gtgctaaagg agctgcaatt
    24781 tctactggaa ctaatgtaga aatagttcaa ttagatgaaa tatacaaaga gattaagaat
    24841 gattctgaat tagtgaatat agttagacaa aattttgaag ttttgggtga agactatgtt
    24901 gaaagagatt taagtcaagg tataggttca actgatacag gaaatttaac tcatgagata
    24961 cctgcaatac aagcatatat aaaattaaaa gaaaatactg caactcatac agatgaattt
    25021 gctgttgcag ctggtggtga agaaggcaga gtagcactta taaaagcaat taaggtgtta
    25081 gcaatgtgtg gagtagatat tttgtatagt aaataatata taattttaaa atatattaag
    25141 aaaggaaatc ggattagaca tgatattaaa acaagtaata gaggtttatg acattttaga
    25201 taaagcaaat gctaatgggg aagaagtaaa aacttactta caaggttatg gtgaagtaga
    25261 tgtaactgta aaagaattgt cttcaagtaa aggctcaaca gatttagtaa agcttacaat
    25321 tcctgggaaa aatggaaaat taaaaggtgg agatgctcct actcttggca ttttaggtag
    25381 actaggaggg attggagcaa gaccagaagt tataggtttt gtttcagatg gtgatggagc
    25441 attggtagca attgcagtgg ctgctaaact tttggatatg caacgaaaag gggatatatt
    25501 agatggtgat gttgtaattt caacccatat ttgcccagat gctccaacta aagaacacta
    25561 tcctactcct tttatggatt ctccaattga tatgatgact atgaatgaaa atgaagttga
    25621 tagtagttgt gatgcaatac tttctataga tactactaaa ggaaatagag taatcaatac
    25681 tagaggattt gcaatatcgc caactgttaa agaaggctac atattaaaaa ctagtgataa
    25741 ccttttagat attatgcaga ctgtaacagg gaaatctcca tttgtattcc ctttgtctat
    25801 tcaagatata acaccatatg gaaataatct gtatcactta aatagtattt tacaaccagc
    25861 agtagctaca gatgcaccaa tagtaggagt agcaattaca acagagatgc ctgttgctgg
    25921 atgtgctaca ggtgcaacta attttagtga tttagattca gctgggagat ttgcaattga
    25981 agttgccaag ctatttggta gaaataagtg tgatttctat gataaagaag agtgggaaat
    26041 gcttattaag agatatggca aattaaatag atttcaaaca tttggtatac agtaattaga
    26101 aaaatattaa gtttatataa tttgtgatat aactatttaa gaatatataa acaattagga
    26161 ataaaagtaa gaataaagca cttagttaaa aactttaaaa ataaaatatt tatttgttta
    26221 tataaaaagg aggattctgg ttattgggat tctctttttt atgaatacaa ataatatatt
    26281 gtaattataa aaaaaggagc gtttcaaagt gattttttta atcaccttga gacaactcct
    26341 tatattttta tttaaattct attttttatt aagttgctgg agttccacca ccatcaggag
    26401 gagtagtaga gccaccacca ttatttccac cattatcagg aggagtagta gagccaccac
    26461 cattgtttcc gccattatca ggaggagtag tagagccacc accattattt ccgccattat
    26521 caggaggagt agtagagcca ccaccattgt ttccgccatt atcaggaggt gtagaaccgc
    26581 caccattatc acctgtgtta ttattgttat tattattttc ttctggttgg ttattgtctg
    26641 gtttttttgt atcttcagat gatggttgag aacttacttt agtaggagca gtaccttcaa
    26701 taaatgctgc tttagagcca gaagattgct ttcctccatc ctctaaatta atttttgtaa
    26761 aatatacatt tttaggaact tcaaattcag taaccgttaa attagcatgt attttttcca
    26821 taattttttc ccatagtttt gcagtactag tacttccatg tgggacttct ctacgtttta
    26881 tagtgttacc agaatcatct tttctaccag catcatctcc aatataagtt gcaccaacat
    26941 aataaggagt gtaaccgaca aaccaagcat catatgcact atttgtagta cctgttttac
    27001 ctgctacagg cattcccttt ggaagtgtag caacaccacc agtaccttca gtaattacgc
    27061 tttctaacat atctgtaaca acataagcta cttcagggtc tacaacttta tgttcttcag
    27121 gagcattttt aactaataat tgaccatcaa aggtagttat agtagtaaaa atagaaggtt
    27181 ctacataaat accgtgattt gccaatgtac cataagcagc agccatttga agtggagtaa
    27241 taccattcgc cataccacct agtgtcaatg atgggaattt tctatcactt gcttcagatg
    27301 aacctgagtg attattcttt aaagttgtta taccaaagtt ctttaagtaa tccatcatta
    27361 tatctacaca ttcatcataa gagtctccaa gcatttcagc agttttgaca gctatcgtgt
    27421 ttgaagattt agcaagtgct ttacgtaaac tcatagaacc atgaccagca gtagtagttc
    27481 ttggattcca ttcattattt tctttaaact tatatcctcc tcgagaatca cttagagcag
    27541 ttgcttgtgt tatttttaga gtatcaatag ctggcgtata tattgctaaa ggttttatag
    27601 aagaacctgg ctgatgaggg ttagtagcac gatttaaagt ttttctacca cttatatttc
    27661 ttcctccagc tagtgctctt atttcaccat ttttataatc taagattacc attgcagctt
    27721 gcggttggct aactccatca ggaccgacca tatgtcctgg gaaattgcta tttctatcaa
    27781 attcttcttc taaagcatct tgcattttag ggtctatagt tgtgtttaca attagaccac
    27841 cattgtagaa taagttttga gcttcatcat ttgtatgtcc ttgttccatt aaagcattta
    27901 taacttcact ttcgataagg tcttctacag atgaagaaac tttatctgat gattgaggca
    27961 attttatttc tattttagct ttgattgctt catcatattc actttgtttt atatatttca
    28021 attctaacat tttctttaaa acaatttctt gtcttttttt agcttcagga ttacttacag
    28081 cctttctaac aactaatgta cctgctttta attgtctata agtatcatta gatataagct
    28141 cccaagattt tattttttca atcatatcaa agtccacttg agtagggtca tctaaatcat
    28201 catcagttgt gttaacaaaa aataataatt tattctctaa atcctcctta gtttcatttc
    28261 cctctaactt agaagtttta taggctgaaa atcttgatgg atttttagtt gaaccagcaa
    28321 gaagtgctga ctctgctaga gtaaggtcag ctgcagactt atcaaaataa ccacgtgcac
    28381 cagcctcagc acctgctaga cctctaccaa cgaagaagtt atttaaataa gtttctagta
    28441 ttttatcttt acttactgtt ttactcatct catgtgcata gtaaatatcc tttattttac
    28501 gaggtatagt ttgctgttca gttgttaaca gcattttgga tacctgcata ggaatagtac
    28561 taccaccttg cttagtacct gtaagggtct ttaatactga cctaaataaa cctttaatat
    28621 cgacaccctt atgctcataa aaacgctcat cttctattgc aattacagca tgttgaagat
    28681 aaggggaaat cttatctaat ggaacgggtg tctttttatt tacacttgga gctgtagata
    28741 atgtttttcc attagcgtat ttaatagtag ttgtttggtt cgtctgttta tctaaaacag
    28801 cttctgtaac tggagttaca tctcttagtg acgcaaatac tagaccagca gtacctgctg
    28861 taccaacaac tagtaaaact aaaaatacta tcccaaatac tcttagtttt ttaaatttat
    28921 cgcttttttt agattttttc ttgttttttg ttgaacgagt cttgttagct gaagttctat
    28981 taattggttt atttgtatta ccagatgagc taactttttt tctcctgatt ttatttccat
    29041 ttttatcatt atcgttattc ataagatttt aacctcctta cttaatgcaa tattataaca
    29101 caaataggag tagttttgta attaatagaa aaaatgaatt tgctataata ttctgtacat
    29161 tgtatatgta taaacgtaat atattaaaaa gtctatattt ttattttatt tatttttgac
    29221 aaatattata aaaattatgc caaagctaca tataaataaa taggagggga taattttgaa
    29281 tagaatagta gatagaaaaa caaaaaatga atttagaaaa atcatagcta tttttttagt
    29341 aattcttttc ttgtctgtat ttataggcag ttttatatat atagataaaa ctttaagacc
    29401 gacaataaca gttttagcag agactaaagc tcttgaacta gcaaataggt cgataaataa
    29461 agctgttgct gagatggtag aaggaaaaat aaattatgaa gatttaatgg atatacaact
    29521 tgataataat ggtaaaataa ctatgataca agcaaacact ataatgatga atgaaattgc
    29581 atctgcaata gctttagaaa tacaagatga attaaaaaag gataagacag caagttctta
    29641 catacctata ggaacagcat taggaagtcc tatattagct aaatacggtc ctaaacttga
    29701 agtatctatt gaacctatag gaacagtatc agtaaacttt aaaacggaat ttgagtcttc
    29761 aggtataaat caaactagac atagaatata tttagaagct caaacacaag ttaaagtagt
    29821 aataccactt ataacttcaa caaaacaaat taaagctcaa atacctattt gtgaaactat
    29881 aatagtaggt gatgttccag aaagttatgt aaatattcca gaaaaaaatc taggaaatgt
    29941 tttacctaac actggaaaga ataccaacaa gtaatataaa gttgtatatt tttaaataaa
    30001 ttcaaaataa tagtttataa ataaaatttt tggaaataat attaatataa ttttaataaa
    30061 aatttatatt ttatactatg taaaatatct aatatatttg gtctgtaggt taaataaaag
    30121 tacctttaga ccaaatatat tattaataac ttaataacat cttaaaactt atttatttat
    30181 ttatattatt tacaacatcc atttaataaa tacaccataa cctacagctg ggtcgttttc
    30241 aattgcatgt gacactgctc cgattatttt attatcttga actattggag taccactcat
    30301 accttgtaca ataccaccag tttgagctaa aagcttttta tctgtaattt taattttaaa
    30361 acttttactt tctggatgtt tttggttttc tatatctata atttcaatat tatatttttt
    30421 acacttatta aagttatctt gtaaaattat ttgtgctttt cctaatttaa cttcatctaa
    30481 agatgctact ttatattttt ttaacttgga aatatcaaag ttattaattt gacctcttat
    30541 tccaaaatca gtattttctg taaagctacc aataatattt tctcgttttg catttataca
    30601 tccaacacta cctttataag atttttctat attaaggtgc ttagttgtag aaattgaacc
    30661 tgttttaata ggtatttttc ttgcattacc aacacttatt ggatgaccta cagctccaaa
    30721 ctctgatgta tctggattga tatacgttaa agtagcaaat ccagaaagta aattgttaaa
    30781 actaattttt tctaatatgt gtttggtagt tttcattgtt ataagttgtc cacctctatt
    30841 taataaaata gatacttgtt tttcttctgg taaagcattt aaggtattgg caaagtcact
    30901 atatccatca acttcagcat tatttacctt tactatagca tcacccaatc taaatggaga
    30961 tgcttctcca ggatttctaa ctatgatatt ttttaattct gcgtcaattt gaagtacgtt
    31021 tccaattggt attaaatatt caggttcatt tttttgtaaa ttttgtgcat aaattaaatt
    31081 atttgagaaa aaatataata aaaataaaaa taaaaatgtt aaaacaattg taaattttga
    31141 cttagctaag ttggtttttt tacttaaagt attattttta aaatgaaaat ttttaagttg
    31201 cataaaacac ctctttctta ttaaattatt ttaataatag aattgaagtt ttcttgctgc
    31261 ttctatatta tgttgtgcaa gaatcatgtt ttgatgcttg gaaaactagc tcaataacac
    31321 cttagttttc ataaaaataa ataaaataaa tagtgtatat gataaaataa atacatattc
    31381 atataaataa aaataaacaa taactgtcta gcataagaaa ggagcaaaaa atgaaaaaat
    31441 atagattgtt agttacttgt ataattgcca tgggaatcat aattatagga acatcaaatc
    31501 cagttagcaa tttttacaag actacaaatt tgattagttc aggaaatatc acaaatgaaa
    31561 aagcaaataa agatgaaaat tcaaacaaaa ataataaaga taaaaatgaa gacaaatcta
    31621 attctaaaag taataaagat aagtcatcag aggacaaatc gtcagataat aaaaatgaaa
    31681 ttcaaaaaaa taaaaaactt ttaatttgta tagacccagg gcatcaagga aaaggagata
    31741 gcaatttaga accagtagct ccaggttctt cctctaaaaa agcaagagta tcttcaggaa
    31801 cagagggtat tgctacaaaa aagccagaat atgttttaaa cttagaagca tctcttgttt
    31861 taaagagtat attagaatct aaaggttata atgtaataat gactagagag acacatgatg
    31921 taaatataag taattcagaa agagcaatac ttgctaatga caaaaaagca gatatggttg
    31981 taagaataca tgcagatagt ttaaataatt cttcaaaaac tggagcatct atattgattc
    32041 ctgaaaaaga tggaaaatat acagctccta tatatgaaga aagtaataaa tgtgctgaat
    32101 ttatcaaaca gaatatggaa caatcaggta tacaaatcaa tggaatcgtt caaagaggtg
    32161 atttgactgg attcaactgg tcaaaagtac cagctgtatt agtagaaatg ggatttatga
    32221 gtaactataa tgaagaccaa atgatgtcaa atccagacta ccaaagaaaa atgatgcagt
    32281 gtatagctga tggattggat gcgtatttta aataagatat gaatagagaa aaagttaggt
    32341 tggtattatt ttcctaattt ttatctctat tttttattgt acataacaca aagaagaagt
    32401 ttattttagg ttaaattgtc gtaaatataa ctaaaaatat tgggaatact tgatttaagt
    32461 ttaaaaagaa tatattatag tatagaatgt gtaaaatttt tttgagaggt gttgaaatgc
    32521 tagtagtaga aaatgtgagt catggatttg gtgcgagaac aatattagaa aatgtatcct
    32581 ttagacttag aaaaggtgaa catattgcac tagttggtgc taatggagaa ggaaaatcga
    32641 gttttttaaa tataataaca aagaagctta tgccagatgc tggaaatata aaatggtctt
    32701 caagagctac agtaggttac ttagaccaac atacggtatt aagcaaggga aaaactataa
    32761 gagaagtgct tagagaggct tttaagcata tgtttgactt agagcaagaa atgattgcta
    32821 tgtatgacaa aatgggagaa gctagtgatg atgaaatgag taagcttctt gaagaaacag
    32881 cagagataca aactatactt gaaaatagtg gtttttatat gatagatgca aagatacaag
    32941 aagttgcaaa tggtcttggt cttggagaaa ttggtcttga taaagatgta actgacttaa
    33001 gtggagggca aagaacaaaa gtacttctta ctaaacttct acttgaaaat ccaactattc
    33061 ttatattgga tgagcctaca aactatttag acgaagagca tataacatgg cttactaagt
    33121 accttcaaga gtatgagaat agttttgtct tagtttctca tgatattgaa tttataaata
    33181 atacatgtaa tgtaatatat catatggaaa atggagagtt aaatagatat aaaggaaatt
    33241 atgatgaatt tgtaagactt aatgatataa agaaacgtca agaagaacaa gcttatgata
    33301 aacaagttga agaaagaaaa agactagagg attttgtagc tagaaataaa gctagagtag
    33361 caactagagg aatggctaac agtagacaaa agcaacttga taagatggaa atattagaaa
    33421 gaccaaaaga aaaaataaaa cctacttttg cttttaaaga tgctagagca gctagtaaaa
    33481 taatttttga aacagaaaat ttagttttag ggtatgatga agcattgaca aaaccactta
    33541 atttccattt agaaagagga aaaaagattg ccttaaaagg aatgaatggt attggaaaat
    33601 ctactctatt aaaaacttta cttggaataa taaaaccatt tgaaggaaat gttaaattag
    33661 gtgactattt ggaagttgga tattttgaac aagagagttc aagagaaaat agtaatacac
    33721 ctatggatga agtttggagt gaatttccag ggcttacaaa ctttgaagtt agacaggctc
    33781 ttgcaaaatg tggacttaca aatgaacata taacgagtca aatgagagta ttaagtggtg
    33841 gagaagctgc taaggttaga ctttgcaaag taatgttaaa aaatataaat ttcttagtat
    33901 tagatgagcc tacaaatcat ttagatgttg aggcaaaaga tgaattgaag aaggctatca
    33961 aagagtttaa gggaacagtt cttttagtat gtcatgagcc agaattttat tcagaaattg
    34021 ttgatgatgt atggaatata gaagatttta caactaaaat cgtgtagtga tttaacttat
    34081 tgctgggggg atttcatgaa aaagtttcta aatgtacttt tttcaatttg tattagtatt
    34141 gttataatag taggtgttat taattttact gttggattta aacaacttta ttattttgac
    34201 attgattact taaatatatc agagttatct ggtttatcta aagatgatat aaaattgaac
    34261 tacgattatc taattgatta taacttaaat aagaatgtgt ctgagttcaa attaccaaca
    34321 ttaaaatctt ctccacaagg gaagatacat tttgaagaag tcaggaatat atttcaaaat
    34381 ataaataaat tagctaaatt attattggta gtttctttag taggaattat attgagtgta
    34441 aaaaataaaa atataaaaat cttaaaaact acatcaataa cacttataat tatgccatta
    34501 ttattaacag ttccaatttt attaaatttt gaaaagtcat ttattatttt tcataaatta
    34561 ttatttagaa atgattattg gatatttaat cccgatttag acccagttat aaatatattg
    34621 ccagaggaat ttttttttca ctctggaatg atgattttaa tattaatttt gttagtaagt
    34681 atagtactgt ttgtaatgta taaattatgt aaatctttat caataaaaat atttcaatgt
    34741 taaatagagg gatattttta tttccaaaat aagataataa aaaaggatta gattaaaagt
    34801 tatacaataa aaatagctgt gcaataatat ttagactatg aaagtagtta aaaaatattt
    34861 atgcacagct atttataata gaatttactt tttactagtg attttaacta aatcttgaaa
    34921 tttactcata atgtaattgt aattatcttt tttatgaggt aatatacaac tactgcaatc
    34981 tttgatacct ttatctgtat acttaaagtt tccaccacag ttttctccta aagcatacaa
    35041 aggacaatag caaaatagac agttaaactc ttctggttta ttagttttat gacaagggaa
    35101 aaattcgcaa tctttgtgat taaaaaactt ataattttcg cccatatttt cctcctaatt
    35161 aaataaaatt taaatttttt atactatata agattttggt gataatataa ttatactaaa
    35221 taattttatg aatagtcgat aatatagtat tattaatcat agtattaatg gtataattag
    35281 tcaacagaaa aataatgtaa aaataatgta agtgtatttt gtaattagaa tatgttttat
    35341 aacaaaaaat gaggtgatta agtgaaagaa attcaagaaa aagcacttct tgtaggtctg
    35401 aatttgacta caatagtgaa aaaaaatgat gatatagaca ctaatgaatc tatggaagaa
    35461 ttaaaagaac taacaaaagc tgctggggca gaagttgtag gtagcttaat tcaaaataaa
    35521 cactctgtag atgcagctta ttatatagga aaaggtaaag tagaagaaat aagagcctat
    35581 agtgactctt tagatgctac tttagtaatt tttaatgatg aattatctgg tgctcaaatc
    35641 agaaatatag agaatgttgt tggaagaaaa gtaatagata gaactacttt aattttagat
    35701 atatttgctc aaagggcact tagcaaagaa ggtaaattac aagttgaatt agcacaatta
    35761 aagtatagat tgccacgact ttatggaatg ggtggtgaga tgagtagaac aggtgcaggg
    35821 attggtacta gaggtcctgg agaacaaaaa ctagaaatag ataagagaca tatacttaat
    35881 aaagcagcag atataagacg tgaacttaaa gaagtaaaaa agaatagaga gacacagaga
    35941 gttaagcgtt taaagtcaaa tataccgatt gtagcacttg ttgggtatac aaatgcagga
    36001 aaatctaccc tgttgaacga attaataaag acacacaagg attatgaaca agaaaaagaa
    36061 gtttttgtaa aagatatgct ttttgcaact ttagatgtaa ccttgagaaa ggctcttttg
    36121 ccgaataaaa aagaattttt agtagtagat actgttggat ttgtaagtaa acttccccat
    36181 gatttagtag aagcgtttaa ggcaacattg gaagaagtac agtatgcaga tttaatctta
    36241 catgtaattg atgcaacaaa tactagttat gaattgcaaa aaagtacgac tgaaggtgta
    36301 ttaaaagaac ttggtgtaaa tgataaaaaa catatacttg tatataataa agtggacaag
    36361 ctagaattgg atatatatcc taaaagtcaa gaagatattg tgtatatttc tgcaaaacaa
    36421 ggcataaata tggataagtt attaaatatg atagaaattg ctttaatgga aaatacttat
    36481 tcagtaagtt tgatgttacc ttatgagaga ggtgatatat ttagtagaat aaaagataaa
    36541 tataatgtgg agaattttga gtatggggaa agtggtataa cgctagatgt agatttagat
    36601 gaagaagatt ttaatatcta tagagagtac atactagaaa aataggttga atatggatat
    36661 aaactgggaa aatatagata atcttgaaga ttattttata acctatcttc tttataaaga
    36721 atctaaaaca gtatctcaaa ttagtaaaat tagaaatatt tcaagtactg aagcaagtga
    36781 gcaacttata caagcaaagt taaaaataaa ggaaatgcaa aaagatgatt ttgaggcatc
    36841 taaggatatt ttggataaat ttctagaact agataaaatt aagagacttg attttatgga
    36901 tagcttagat gacgaaaaaa tggtatactt taagagaaag gttttcaaaa gaatccttgt
    36961 agaaaaaaat gctgaagatt taattgtttt aatttgggcg acaggtgagc taaaagatga
    37021 taggtttttg aaattgcttc atcagctcac aaaccacaga cactcagata ttagaagaat
    37081 aacttattca gcaatcagaa agattgaatc accaagtagt agagaagtac tgcaaaaagg
    37141 tctctacgac aaaaatgcac agactaggca gtactgtgca aaagctcttt caaaattggg
    37201 agatgaaaac agtttaaaga ttttacagca gttgaaagac aagaataaga attttgaaaa
    37261 agaatatgtg cttagagctt atgacaaagc cattaagtca ttggaaaatg caaaaaattt
    37321 gaaaaagtaa ttttttatta aagcaatttg tttacttaat gatgtatttc taaagtttaa
    37381 caaggtaata aataaattaa tgggaggggt ttcaaatgat agtaagacgt tgtgcaggtg
    37441 gagtagtgtt ttatgctaat aaagtactta ttgtaaaaaa tgataggggt gaatggacac
    37501 ttccaaaagg aaagatacta ggagggggac ttccatatga gagtgctgtt caaagagtta
    37561 aagtagaaac tggcatagat gctaaaatga tagatgtggc tggcgatact atgtatgaat
    37621 ttttctcaag aagtagacaa caagaagttt gtaatgcaat aatgtggtat gttatggaag
    37681 cttgtaacac tgattgtgta cttgctcctg aatttcaaga aggtggattt tataaagtaa
    37741 aggatgcttt agaaatgtta tcacatcata aagagcaagc tttagtagaa gtatcttata
    37801 aaaagtttaa ggaattaaag aaattagttt cagagggcga tgatgttcca gcaaattaat
    37861 tgtgaattta gaggattctg aggaagggat agccccttcc ttttttaatt gaagcagttt
    37921 taagcgtaaa ttatatagtc tctttaaact atgatttatc aatttagaga tttagtagtt
    37981 tatatttaaa atcagtaaga tatatttaaa ataaaaatta aaaagtatac aattttatta
    38041 tataattgtc ttatgatgtt gattaaagaa ttattaaaag tccatttatt tttaaataga
    38101 ataattttat aaatatatat ttgatgtgta tattagaaaa tgattaaaat aggaggaaat
    38161 acaatgagca attacaggac attacatgaa tttggaatgg atgaaataac gattgaaaag
    38221 tctgttttta ttggatatgc taagcctata aagagtgaag aagaagcatt agagtttata
    38281 aatgaaataa agaaaaagca taaagatgca actcacaatg tgtgggctta tactgtaggt
    38341 aaaaatatga atatacaaag atatagtgat gatggagaac cacaaggaac agctgggata
    38401 cctacattag aggttataaa aaaagaagat ttgagagatg tagcggttgt tgttactagg
    38461 tattttggag gaacaaaatt aggtgcaggt ggtctagtta gagcttatac aaaaggtgct
    38521 aaactgggac ttgaagcagg taagataatt tataaggtaa tgtatcaaga agttaaggtt
    38581 aagatagatt atactcaact tggaaaagtg caaaatgagt taatgaattt ggggtatttt
    38641 ataaaagata ctgtatatga ggataatgtt gaaatagtag tatattcaag attagaagat
    38701 gtagagaaat tatcagaaaa aatgattgat ataacaagtg gaacaggaaa aatagtttta
    38761 ggagaggaat tttatttgtc tgaacaaaat ggagaaattt taatataatc taaaaggtta
    38821 ttagaatagt taatacctca aatagccaat tagtattttt aagtgaaaag ccattaaatt
    38881 gatttaatct aaatcaattt aatggctttt tatcggattt tgcacagaat ccttttttta
    38941 cttcaaataa accgactaaa agtaaaaata tcccaaatat tttttttaat atatctgaag
    39001 acagataatt agctaatagt gaaccaaata cagctcctaa aaccccaaac acagctatag
    39061 gtataaccaa tttaaaaatt acatttttat tttttatatg tatagcaagt gctataagtg
    39121 tcatagggat agatgaaagt aaatttacgc tttgtgctat tttagtgtct atgccagcaa
    39181 gtaaaacgag tgcaggtata aggattgttc ctccacccat tcccattcct ccaataattc
    39241 ctgcaaaaaa tccaattaga gcaaataaca ttaaaacacc atccttaatg ctgcaattat
    39301 cataattatt ccaaaagaaa ttctaagaaa ctttccagta accttattta gtaatttagc
    39361 tcctataata ccaccaagaa tacttccaac tgcaacttta attgttaagt taacatcata
    39421 agtaccttta gatacatata aaacagaact tgctgtcgtt aagaatatta ttatagaaag
    39481 agcagttgca tgagatttat gttcttctac cttaacgatg tcatttaata ttggaactaa
    39541 aagagtacct cctccagaac caaaaactcc atttataaat cctgtaaaaa ctccaattat
    39601 cgtatttttt aagttaaaat caaagtattt tttctttaaa tcatcagttt ttttcataaa
    39661 aatcaccttc tgaaatagtt ttaccaaaat aggcaaatta tatttatata aaataagatt
    39721 aaatataatc tttcatgttt gtttaatttt gcataaaaaa aaacatatta aaacaacata
    39781 ttaatatatg gtataatata ttaaaacaaa gtagcaaatt tgagaggaga taacaatgtc
    39841 aaatataaaa atacaaatag acaaaactgt agaatggctt ataaataagg ttaatgaggc
    39901 aaatgcaaaa ggattaattg ttggtgtatc tggaggaatt gattcggcag tagttgctaa
    39961 tttaattaaa aaggcatttc cagaaaactc tatgggagtt ataatgagca taaaaagtaa
    40021 tcctcaagat agagaagatg cacttaaggt tatagaggga tgtgatatag aataccttga
    40081 tttagacctt atagaaccac aaagtgctat attagacatg gtagtaggaa atttaaaaga
    40141 taaacattta tatagagaag aatatttaaa gatgacagat gcaaacttaa gagcaagagt
    40201 aagaatgagt acaatttata caattgctaa caatctagga tatctggttg ttggtacaga
    40261 caatgctgca gaaatacaca caggatattt tactaaattt ggtgatggtg gagttgatat
    40321 tttacctatt gcaaatttaa ctaaaggaga ggtgtatgaa tgggcaaaag agcttggtgt
    40381 tcatgaagat ttaataaaca aagctccttc agcaggtctt tgggaaggtc aaacagatga
    40441 agatgaaatg ggaactactt acaatatgat agatgctgtg ttagaaggta gattggatga
    40501 agttcctaaa agagaccaag agataattga aagacttcat agattaagtg aacacaagag
    40561 aaaaacgcct gcacaacctc ctaagttcta atttactatt tataagattt gtgataaaat
    40621 ttacaagtga tgaaaattag aataattaat tgtacgtatt aaggagtgga aatatgggac
    40681 gtataggtaa tataattaat agaaaaggta aacaagatgc tcaaagagca aagatattta
    40741 ctaaacatgc tagagcaata gcagttgcag ctaaggaagg tggagcagac ccagagtaca
    40801 atgcagcgtt aaaaacagct atagaaaaag ctaaggcaga taatatgcca aatgataata
    40861 tagatagagc tatcgctaaa ggtgctggag caggtgctgg agaagactat gaaactatag
    40921 tttatgaagg atatggacct ggtggagttg ctgttatagt agaaacttta actgacaaca
    40981 aaaacagaac tgctggtaac gtaagatact attttgacaa aaacggagga aacttaggaa
    41041 ctagtggatg tgtgtctttt atgtttgata aaaaaggaca aatattagtt ggtttaggag
    41101 atggagtttc agaagaagaa ttgatggatg ttgcattaga agcaggagct gaagatttca
    41161 taacagaaga agatggttat gaaataataa ctacaccaga agatttttct agtgttcgtg
    41221 atgagttaaa agctaaagga tatgagttta tatctgctga tgtgaagatg atacctcaaa
    41281 ctacaactgt attgactgaa gaatctcatt taaaaatgat gaataaatta gttgatatgc
    41341 ttgaagaaga tgatgatgtt caagatatat atcataactg ggaagtagaa taaatagtat
    41401 aagcatattg aaatttataa gtattgaatc aattttaaca tgtgataaat tttatagaat
    41461 aagaaacact aaaatagaca tacgcacctc taacctatat ttgcataata atatgtaagc
    41521 ataggtaagg ggtgttttta tatttatagg ttaagaaatt taaagtaaat tttgagctgg
    41581 actcgattgt ctataaataa ttgatgggta tataacttgt tgctgaatac catttatacg
    41641 cccagttact gtatcaataa tcatcctaag actacgttct gctgtctctt ccatttttaa
    41701 atctataact gtcaaaggag gaatacttct tgccattagt cgtgaaggtc cattacttgc
    41761 tgcaaatatt tgaatatcct ctccaattac aattcccgat tcatgaaatg cattcatcat
    41821 accaagagct accacatcat gattacaaaa aattactttt tttagtgaat tttttgcaag
    41881 cattttttga gcaatatcat atccactatc tattttatta gaacaataat atatagaatc
    41941 tgatagatgt attccatgtt ctttacatgt ttttaaaaaa gatttacccc ttaaagtcat
    42001 acatgtaaag gaagttgggt tgaatacaac agcaatatca tttttaccat tttgaatagc
    42061 atgtaaagct gctatacgac cagtttcctc attatcaata gatacacttg aaaaaccagg
    42121 tatttcacga tttacaacaa ctacaggaat tttaggttta ttttttacta gatattctat
    42181 atcatcagca ttgatacctg ttaaaacaat tccatcataa taattatttg caaaaaagta
    42241 ttcatcatca catagatgac caactttgta aggtcgtatg atgatattta tgtttaaatg
    42301 ttctaaagaa attatgtggt ttattgcttg aagtatattg gttgatgcca actcaaatcc
    42361 ctcatcctgc caataaaaag ctattttaat attattatgt ttaataggag tagagcgttt
    42421 atatccaagc ttatcagctg aatttaatat aagctcttgt gtatgtagcg gaatcctgcg
    42481 ttcatttcct ttaccactta agacgtacga gacagttgct gaagaaagac cacagtcttt
    42541 tgcaatatct ttgattgttg ccatgataaa atcctcctta aatagttgct taaatttgct
    42601 tataccaata atcttaacat atattcaact aaagaacaat attctacaag gtgcatattt
    42661 tacagaatta gaataaataa atttaaaaaa acttcttact tatgttgaca taatttagtt
    42721 tataggatta taatatgctt aaataagctt attattaact taaattaagc aaagttaagc
    42781 aggaacatgc aatgctagct tgcaatgtga ctgtaaaggg ggaaaaggta tgagtaaggt
    42841 aaatatattt gaagttggtc ctagagatgg atttcaaaac ctgaaagagt ttttagaaac
    42901 tgataaaaag cttaagatta ttgatggtct tattgcttca gggattaaac aacttgaaat
    42961 tagttcattt gtaagcccaa aagcaatacc tcagatgaaa gatgcaaaag aagtagcaac
    43021 ttattgtgta gaaaaatatc ctgatgtgaa actttatgca ttagttccaa atttatacgg
    43081 tgctaaagtt gcatgggagt gtggtataag agatatatcc tatgttatat ctttgagtga
    43141 aactcataat aaaaaaaata taaatcgtac ccatgaagag tctattgatg agcttaaaaa
    43201 aataatgaat acttatccag acatgaatat atgtattgga atggctactg tatttggatg
    43261 tccttttgaa ggtttaccaa aaattgaaga tgccataaga tttgctaaac gcctttggga
    43321 aaacggtatc agggagatga atctagcaga tacaataggg attgctgacc ctaagcaagt
    43381 aagagaaact attagtgcat tagttgaagc tttaccagag tgcagatggc aggtacatat
    43441 tcatgatact agaaatatgg gaatggttaa tactcttgtg gctattgaat ctggaattac
    43501 tactgttcag tctacacttg gaggattagg aggatgccca tttgctcctg gagcatctgg
    43561 caatactgca actgaagatt tagtatatat gcttgaacgt atggggtatg atacaggagt
    43621 tgattttgtt aaacttcttg aattatcaaa gtatcagaag agttttgtaa atggagtata
    43681 tagtggtcat catgtaaata taaagtctga tagttgtaat tgttagttct attgattaat
    43741 cgattaaaaa aatatgttga ttaaaaaaat atgctgattg gtaaaataga tttattgata
    43801 aaaaatataa attataaaat acattatcta ataaaacaaa taaaaaaatt gagggggatt
    43861 agtatggata ttatagcttt acttttattt ttaggagtta ttattattgc atttgtacgt
    43921 aagaataatg caggtatttt ggctctagct attggtgtca tagctgttag aatttttgga
    43981 atggatgata aagaacttta tggagctata agcaactcaa tgtttactac acttgttggt
    44041 ataacactac tctttgcaat agtgaactca acaggagcat taaagcttgt tgcagaaaaa
    44101 attgtagctg cttctggtaa acgtgtttgg cttataccta tagcagtttt tatagcagga
    44161 tttgttgttg ctggtgttgg acctggggca attccagcac ttgcaattat cccagcaatg
    44221 gctgttcctg tagccataac ggttggttat aatcctgtaa tgcttgcact tataggtgaa
    44281 tgtggattaa tggcaggtcg tatgactcct attactccag aaggtcaaat tataacaacc
    44341 gttgcagaag gggttggaat tgaaaatgtt atgcctacta tcttagcctg tcaaactctt
    44401 tctacaattg tattttcaat agtattgttt ataattttca aaggatataa acttaaaaaa
    44461 cctataaatg ttatagatag taagcaaata gaaaaattca atataaagca gataatttct
    44521 ctaataagca tagtagttat gattgctttt atcataattt ttgatatgaa tgttgggctt
    44581 gcagcttttg cagtttcagc tgtacttatt tttttcaata ttgctgatga aggagaatgt
    44641 atcaaatcaa ttccatggag cacgatagta atggttcttg gagtaggtgc aatgatgaca
    44701 atcgtagatg cagcaggagg tattgacttg atgagtaatg cattatcatc actgatgaat
    44761 agtaaaacag ctactccaat tatgagtatt tcagcaggac ttatgtctat ggttagttct
    44821 gctttagcag tagtttatcc aactatgatg cctatgtgtg ttgatatagc taaggcagtt
    44881 ggaggagtag acccactagc acttattgct gcagttggtg ttggaggttc tcttgctggt
    44941 gtaagcccac tttctactgg tggggcattg attttagcag ctatgggttc aagtaagaaa
    45001 gactttaata aggaagaaca aaataaggtt tttgtacagc ttttcattat gagtgcagtt
    45061 ggcttattgg ttattgcagt agtatcaata ttatcattta atgcaataac acatttaatg
    45121 aactaattag aataactgaa aataaatcat taaatttaac ttttaatatt aatcaatata
    45181 atataatttg gaggggaagt tatgaaaaaa ggagcattaa gcaatataaa ggtacttgac
    45241 cttacaagag ttcttgcagg tccatattgt acaatgatgt tggcggattt tggagctgat
    45301 gttataaaaa tagagatgcc aagaaaaggt gatgatacaa gaggttttgg accttttgta
    45361 aatggagaga gtttatacta tgcaaatata aatagaaata aaaaaggtat cactctaaac
    45421 cttaaatcag aagaaggaaa aaatatattt ttagaaatgg taaaacatgc tgatgttgta
    45481 gtagaaaatt acagaccagg tgtaatggaa aagttaggtc tgggttatga tgagttggct
    45541 aaagttaaca atcaaataat ttatggagct gtatcaggat ttggatgtta tggaccatat
    45601 tcagaacgtc caggttatga tattattgca caggcaatgg gtggtcttat gagtataaca
    45661 gggcaagaag gaggagaacc aacgagagtt ggtaatgcta tgggtgatgt actaggtgga
    45721 atgaatttaa ctattggtat actcacagca ctcaacgcta gaaaaataat aggaaaagga
    45781 caaagagtag atgtttccct cgttgattct gttgtatcgt ctctcgaaac tggtatacag
    45841 agatactttg attcaggaca acctgaactt atgggtaata gatatgcttc agcctatcct
    45901 tatgactctt ttaaggcaag tgatggaagt tttgtaatag gctgtggtaa tgacaaactt
    45961 tttgaaaagt tatgtaaaaa agttcttttt agagaagacc ttttaataga tgagagattt
    46021 aaaacaaatc ttaatcgttg tgaacattac aaggctctta aaccagaaat agaaaaatgg
    46081 tctattaagc atacaataaa agaagcagtt gacttaatat cagaggctgg agttcctgct
    46141 gctcctatct ataatcttga ttgtgttaca acagatgaac atatagcaaa agcaagagaa
    46201 atgattgtag atattagtca tcctattata ggaaatatga aagtcaatgg aaatccagtt
    46261 aagttgatga caacaaaagc taaaattgat atgccagcac catctctagg ggaaagtaac
    46321 gaggagattt ataatggttg gcttggtttt gatagtgata aaataaaaga tttaaaagaa
    46381 aatggagtta tttgattaaa aaatagctaa acacacttgt gactttagtc aggggaggtt
    46441 cagttagaat aatatcaaaa aatattaaat caatactgta aatcaaatat agaattgtca
    46501 gaagcattta agatgggaaa agctacttag acattgagtc tttgtggctt ttcctatttt
    46561 cattaagaat tttatattat cagaatagat atgtactaaa ctaaagtatt aacaatttaa
    46621 attaatagaa aataaactac ctaattatgt tgaataaaga ctactttagt gaaaataaac
    46681 ttatagagat gctatttaaa ttattttaat ggaaatatca aaaatgagac tgcattctca
    46741 aaaatgagac tgcgtttttt aagtattttc tcgaaaatga gacaaaaata ttgttttatg
    46801 ggttttattg aagaaaaatt cttctgtgaa ttacaattta atcaatttta aagttgaaaa
    46861 ttattaaaaa tttagtgaaa tttatgtata tttcgtttat tttattaaaa ataacaattt
    46921 tttactaaaa ataaagaact tcaatggtga ttggcatagt acttgctatt ttatattaac
    46981 aagaaatgta gaaaaaataa caatgtataa tggggggaat atgatggaat ctgtaatgga
    47041 aaatttgaac aatcttaagg tggaggttaa ggacaaagtc tgtgttatta ctataaatag
    47101 acctaaagct ttaaatgctc ttaatagtga cactcttagg gaactatcac aagttattga
    47161 tgtagtatca gaaaatgagg caatattagg agttattatt actggagaag gtaaagtttt
    47221 tgtagccggt gctgatatac gccagatgca aaactataaa agtgaggaag gcagaaaata
    47281 tgcaggttat gctcagggag tttttgataa aattgaagct cttgaaaaaa cagtaatagc
    47341 agcagttaat ggatatgcat taggtggtgg atgtgaactt gctatgagtt gtgatattag
    47401 aattgcatca gaaaaggcta tatttggaca acctgaagtt aatttaggag ttattccatg
    47461 ttttggtgga actcaaagat tatcaagact tgttggaact ggaatagcta aagagttaat
    47521 ttttacaggg agacaagtaa atgcagaaga agctaaatct attgggctta ttaataaagt
    47581 ggttccaagt gatttacttt tagaagagtc tatgaagatg atgaaccaaa tagttgaaaa
    47641 agcgccaata gcaataagat atgcaaaagt tgttattaac aaagggatag atatggattt
    47701 aaagaatgct cttgaactag agaaagatat tgcaggttta acttttgcta caagagataa
    47761 gcaagaagga atgaatgcat ttatagaaaa gagaaagcca gtttttgaaa ataagtagct
    47821 attataacta aaattggaac aatgtagaat atattcaaaa tctaaatttc gtgaaaaaat
    47881 acatatagtt taattttaaa aggagttaat ttatgaatac gggagtaatt ataagtttag
    47941 tagggattat catagctcta tctcttctag ttgtaatggt tatgaaggga ataaatattt
    48001 ttattatagc aattgtatgt tcatcattag ttgctataac aggaagccta aatgtgtatg
    48061 atgcacttaa agtggattat atgacagggt ttgttggatt tcttcaggca aatttcttta
    48121 ttttcctaac agggacatta atgggaaaaa taatggagat tactggtgga gcaaaatcta
    48181 ttgctaagat gattgtaaga tggattggga aggataaagc actactttct attccaatag
    48241 cttgtggaat attagcctat ggtggagtaa gtgttttcgt tgtaagtttt gcagtgtttc
    48301 cgatagcttt agaaattttt aaagaagctg atttgccacg aagatttata ccagcagctt
    48361 tgacttttgg atgctcaact tttgctatgg ttgcaccagg ggcaccacaa attcagaata
    48421 ttgttccagc atcaacatta gggacagata ttatggctgg agcagtaaat ggttttatat
    48481 catgtgcagt aatgtttata gttggaagta caattttgta caaaatggtt tcaaaagaaa
    48541 aagccaatgg tggtcatttt atagcacatg aatcagatat atttgatgaa gcagaagcaa
    48601 ctaattctaa gagagaaaat ggtcccaatg gtgtaattgc cttaattcca ttgatagtgt
    48661 caattttaat aattaatgta aaagttaatg gaaaagcgat agtaccaatt gaggtgggag
    48721 tatttattgg agctttatta gtatatttat tgttaaataa atatcaagat aatactaaaa
    48781 tagttggaca tgttggtgat gcatgtaaaa ctacagtagt agcaatatgt aatacttgtg
    48841 ctgtagttgc atttggttca gtagtaaaat cagcagttgg ttttgacttc atagttaatg
    48901 ctatgactaa tattcctggt cctcctattg caggggctgc agttggaact acagttattg
    48961 caggtatttg tgggtcagct tcaggtggac taggaattgc agctccactt cttggaccag
    49021 tatttttagc caagggtgtt tccgctgcag cattaactag gactatggca atttcatcag
    49081 cagcattaga ttcacttcca cacaatggat atattgttac tgtaacaaat ggtctttgta
    49141 aagaaacaca taaggatgcc tatatgccaa tattctgggt aactgtttta accccattta
    49201 ttggaaccat agtagcagtc ctactgttta caatgttccc gggattgcca taagagaaaa
    49261 gataaaaggt ataaattata ttttgatgtt ataaaatcaa ttttcaaaat atatacaata
    49321 aatttcatgt tttaatactg taaatatgta tttaaaataa caaattgacc atataaggag
    49381 gaattgaaag tgttaaataa accattagag ggagttaagg ttattgacct aacgtatttt
    49441 gtagcaggtc cagggacttc taaaatatta gctgactggg gtgctgacgt tattaaggta
    49501 gagccaagct ttggagaccc aggaagaaag acaggggcaa caatgacaat gccaatagat
    49561 gattataata atccttttta cagcacatat aattcaaata aaagaggttt gtctataaat
    49621 cttaagagtg aaactggaat tgaaattatg gataagcttt taagcgaagc aaacgtattt
    49681 gtttcaagtt atagaacagg agcattaaaa agactaggtc ttgattatga atcattaagt
    49741 aaaaagcatc cacaccttat ctggggtcag ataaatggtt ttggcgattt tggacctgct
    49801 aaggataatg caggatttga tacagttgcc ttttgggcaa gaagtggggc tatgcttgat
    49861 atagcagaaa aagatacaag tccaataaat ccacctatag cctttggaga tgcaacaact
    49921 tcttgtagtt tatctggagg aatatgtgca gctttgtacc agcaagctaa gactggaaaa
    49981 ggacaaaaag taatggcatc tttatttggg caagcaatat ggaatgcatc atctttatta
    50041 gcatctacac agtttagtga tgagtatcca aaaactagaa aaaatgctat ttcaccagta
    50101 ataaattctt atcgttgtaa ggatggaaag tggatattct taagtatttt agagcatgaa
    50161 agatattttg aggcactttg tagattattt ggaagagaag acttaataaa agaagagaaa
    50221 tttgcatctt cagtagaaag taagaaaaat gctacagaac ttattaatat tttagatggt
    50281 gaatttgcaa agtttactca aaatgagatg gttgaaaaac ttactgctgc tgatatagct
    50341 catgaaaaaa ttcaacatgt taatgatgta gtgactgatg aacaggctat tgctaataac
    50401 tatatatatg aacatacaag taaagatgga aataagacaa tgcttgcatc tactccagtt
    50461 aaatttggaa atattgaagt taacatgact tgtgatgctc cacttatagg agaacattca
    50521 gatgagatat tgagagaact tggatacagt gattctgata tagaggctct aattgaatct
    50581 ggaattgtaa caatgaataa agaagcatgt gaaatatgtt aataaacatg tgaagtatgt
    50641 taataaatag gagggaattt tatggatttt agattaacag aagcacaatt aatgcttcaa
    50701 agagtagcaa aagaatttgc agagaatgaa atagcaccaa tagctgctga aacagataaa
    50761 acaggtattt ttccaaggga gctttttagc aaaatggcta aaataggatt taatggaatt
    50821 ggaacacctg tagagtatag tggttctggt ggggcagata tagaaaaggt aattgttgtt
    50881 actgagattg ccaaaaaatg tgcagcatct gcagcaatat tatcaataca cacaatatat
    50941 gctcaagcta ttttgaaatt tggtactgag gaacaaaaga aaaaatacct tccaatgatg
    51001 gctgaaggtg ggtgtgtagg tgcttttgcc ttaaccgaac ctaatgctgg ttcagatgct
    51061 gcgagagctg caacaactgc aattattgat gaagaaactg atgaatatgt tttaaatgga
    51121 actaagtgtt ttatttctgg tggaggtcaa gcagaatcat taataatatt tgcattgaca
    51181 gacccatcaa agggaataaa gggaatgtct gctatcattg tcgacaaagg tacgccaggt
    51241 ttttcaatag gaaaaattga agaaaaaatg ggtattcatg gttcagaaac tgcagagtta
    51301 atatttgata actgtagagt tccaaagtct aacttattag gaaaagaagg taaaggattt
    51361 aatatagcta tgacttgttt agatggagca agaattggag ttggagctca agctgtagga
    51421 attgctgaag gggctttaga agaaagtatc aaatattcta aagaaagagt ccagtttggc
    51481 aagccaattt cagctttgca aggtattcaa tggtatatag cagatatggc gacaatggtg
    51541 gaatcagcaa aattattagt atattatgct gcagatttga aagctagagg agaaaaacac
    51601 actaaagaag ctgctatggc aaaatacaat gcgtcacgta ctgcaagaga ggttacaaat
    51661 ttagcccttc aaatccatgg tggatatgga tatatgaagg attatccatt agagagaatg
    51721 tacagagatg ccaaaataac agaaatttac gaaggtactt cagaaataca caaagttgtt
    51781 atttcaagag cagtactagg ataggaggaa aagaatgaat atagttgttt gcttaaagca
    51841 agttccagat actaatgaag ttaaaataaa taaagagaca ggtactctaa taagagatgg
    51901 tgtaccaagt ataatcaatc cagatgatag aaatgcttta gaagaagctc taaaaatgaa
    51961 agatgaacta ggagcagtga ttaaggttat aagcatggga cctcctcaag ctaaaagtgc
    52021 tttaaaagaa gctttggcaa tgggagctga tgaagcttat ttaatctcag atagagcttt
    52081 tggtggttca gatacttggg ctacatctac aattattgct gctgctatag agaaggttgg
    52141 aaagtatgat gtaatattct gcggaagaca agctatagat ggagatacag ctcaggttgg
    52201 accagaggtt gcagaatttt tagggatacc acaagttact tatgcgaaag aagtaaaggt
    52261 tcaagatgac aaattactag taactcgtta tacagagaca ggggattatt taatagaagc
    52321 aaagatgcca gttttattaa ctgctataaa agagttaaac aatccaagat atccaagtgt
    52381 caagggaatc ttagaagctt ataataatgg agatgcaaag attactgtat taacacttgc
    52441 agatttagat gttgatacaa ctcaaattgg attaaagggt tcacctacaa acgtttataa
    52501 gtcttttgta ccagttaaag acaagcataa tgaaataata gaaggtataa ataaaaaaga
    52561 aaaagctgag aaactaatag aaatactatt cgatttgaaa ctagtatagg agtgttaaga
    52621 tgatgagagc aaaagttaat caaggtataa atttaaatga ttataatggt gtttgggtaa
    52681 taggagagca aagggaaggt aaaattaatc ctgttacgat agaacttatt ggtgaaggaa
    52741 gaaagcttgc tgaccaatta ggaaaagagc ttgcagttgt gatagctgga tatgaagtag
    52801 agaaagaagt gaaggaatta cttcactata gtgttgataa agtatattac atcaatgacc
    52861 ctcttcttaa agatttcact actgatggat atgcaatttc aattgcaaat ttaatcgaaa
    52921 gaaaaaagcc agaggttgta ttggttggtg ctacttctat aggaagagat atagcgccta
    52981 gaattgcagg taaggttgga acaggtctaa ctgctgactg tacaaaactt gaaatagatt
    53041 caacagataa taagttatta caaacaagac cagcatttgg tggaaattta atggccacta
    53101 ttgtttgtcc aaaaaataga ccacagatgt caacagttcg tccaggagtt atggcaaaag
    53161 ctgtaagaaa cgaatcagag actggaattt tagaagttgt tactccagaa cttactgaaa
    53221 aaatgattag aactagatta gtagagatac ttccacaaga aaagaaatct gtaaatctaa
    53281 ctgatgctag aataatagta tcaggaggaa gaggattaaa aagagcagaa ggatttgagc
    53341 ttataaaaga attggctgat aaattggggg cagagattgg tgcttcaaga gcagcagtag
    53401 attcagggtg gatagaacat tctcatcaag tgggacaaac aggaactact gttagaccag
    53461 aactatacat tgcatgtgga atatctggag caatacaaca tttagcaggt atgagtgatt
    53521 ctaaatatat agttgctata aataaagatg caaaagcccc tatttttagt atatgtgact
    53581 atggaatagt aggagattta tatgaaatta tacctgaaat gatagaatca ttaaataggt
    53641 aacttttaga aaacattgaa tatgtagaat gataagtctt ataatattgt attgtaagac
    53701 ttattatttt tgctgaaggt tatagtgggg agatgaaaca atgaaagagc agtggtataa
    53761 ggatatattt gcaagagtat tatctatgac tgatgatggt tttattgttg taaatagaag
    53821 tggagtaata atagatatta atgataaata ttgtgacttt ttaggaaaag aaagaaaaga
    53881 tataatagga caaaatatac aaagtattat accaaataca aaaatgctag atgttatgaa
    53941 aaataagtat tgtgaagaag gagctataca tcattattca ggtggaaata caaaagaaaa
    54001 aagtgtaata gtaagtaggt cttatgtgga aaatgacaat ggagaagtag ttgcaggagt
    54061 agctcaagtt aaatttagat tgcaatcatt tgatgtagct aaaaagttaa tgtcagaata
    54121 tatggagctc caatattata aagagcagtt taaggataac tgtggttttg acaagctcat
    54181 aggtgaaaat agagacttca tagaacttaa gaaaacaggt gtgaaagctt ctaaaacaaa
    54241 ctttccagta cttttaacgg gtgaaacagg tacaggaaaa gaagtctttg ctagagcaat
    54301 tcacaataat agcagtaggt cagataaacc tatggttagt ataaactgcg ctgcaatacc
    54361 agaagaatta ttagagtcag agctatttgg ctatgacgaa ggagctttta caggagcaaa
    54421 aaagggtggt aaaaaaggta aatttttagt tgcgaataat ggtacaatct ttttagacga
    54481 gataggtgac atgcctttga ccatgcaagc aaaactttta agagttcttc aggaaagtga
    54541 gatagaacca gtgggaggtc ttaaaactat aaagatagat gtaagagtaa tctctgccac
    54601 aagaaagaat ttaagtaaaa tggttgaaga gggattattt agagaagacc tatactatag
    54661 attaaatgta ataaacattc atatgatgga attgaaggac aggcaagatg atattttatt
    54721 gctagcaaat tatatattaa acaaattaaa tgttgaatac aaggaattga aggttttaag
    54781 tgataaggtt aaaaattgtt ttattaacta tacttggccg ggaaatataa gagaactaca
    54841 aaatgtaata aaaagtgcct atgctgtaag tgatgatatg gtcattatga tgtgtgacct
    54901 tccatctaag atggataata tcagcagagt agctcaatgt aatgtggata gcaattgttc
    54961 aattcatgaa atggttgaga attatgaaaa gagcttaatt attgatgtgt taaggaagta
    55021 taattggaaa tgttctaagg cagcagaggt tatgggaata cacaagagtc ttttatataa
    55081 gaaaataaaa aagtatgaaa ttgaattaaa taattaagat aaattgaatt aaggcaatgc
    55141 taaaaaaacg cccttattca aagcgaactg tataaactgt tttatgcagt taaaacgtgt
    55201 aaaagtagat gtgaagtact aatgtcaatc attagtactt ttttattata tttataatta
    55261 ctatctatat ttcagtatct tttgatatct aagattatta tgtttaattt aggatataac
    55321 aaaaccctct tagttatgga cagtttttta tattttaact gtctactaag aggggatagt
    55381 ttagtttaaa tttagagtaa attttatcta gcatttataa aaaatatttt gaatagcttt
    55441 tttgttattt aatcataatt ttctacttct ttatcaagtt ctttgccaca agaatcacac
    55501 ttatatatag cattgtatcc gtaatcatat tcatcaacta ttttcttttg tctaaataca
    55561 ccaccacact cacataggaa accaattttt ataagtgtaa cgataaattg tgattttata
    55621 aattttatat cattacattt atcgttagtg taaaatgtat aaatataaga gttatcgcac
    55681 tcatctctta attcaaatct ttcaatatat gtgttatttt ttaattcctc aaattcttct
    55741 ttagttaaat atgatttttg ttgcaattca tgtaaacgct taatttggtc cattatttat
    55801 atcctcccaa taattttagt attttaagct tagataagga atctaagttt tattaaattt
    55861 atcccaagga attatttaaa tcatatttag ttttattaat tacaacatat ataaattaca
    55921 cgaaaataaa actaattgta aaaataatat cctgtgaaaa cctaaactac taacccctta
    55981 ctaacccgaa aactaaaaaa agaaaatata tccaaatata agtgatatat aaataaagat
    56041 gtttcatata aatttaactt taaatattct gacaataata tatataaata tattatagtt
    56101 aaaatatacc acatattttg ggtattgtat actggaatta gagtaattaa tttatttttt
    56161 aacatacaaa tgattttatg aaaaaaaaca agactcaaaa atttttatat tctttgatag
    56221 aataaaattg agagtaaatt tattgaattt tataaaattt ataaggggtg agtctaagat
    56281 atgcatgaag ataaaaatga tatatttgaa tcaatagata ctgaggattt tgataataaa
    56341 acagaagaag aatgtttaga gctagtaaga aaaaatggat tgaatttaat atacataaaa
    56401 aatcaaacag aagagatatg tctagaggct gttagacaaa attgtaatgc aataaaatgt
    56461 gttcagaatc agacagaaaa aatatgtata gaagcagtta aacaagattg gagaatgtta
    56521 gaatttgtag aagagccaac agaagaaatt tgcatgttgg ctattaacca agatggaaca
    56581 gcattaaaat atatagaaaa tcagacagag gagttatgct taagagccgt tgaaaaaaat
    56641 ggtgcagctt tggagtatgt aaaagaacaa acagaagaaa tatgtataga agcagttaga
    56701 aacagcgaat ttggattggc aagagtgaaa aatcaaacag aaaaaatatg tatggaagca
    56761 gttaagcatt gtagctataa tctaaaatgg gtagaaaatc aaacagaaga aatatgtatg
    56821 gaagcagtga gacaaaatgg gcttgatttg aaatttgtaa aaaatcaaac agaaacaatc
    56881 tgtttaagag cagtaagaca aaatggaatg gcattagaat ttgtaaagga acagactgtt
    56941 ggtatatgct taaaggcagt aagacaatat ggaatggcat taaaatttgt aaaagaacaa
    57001 acagaagaaa tttgtacaga agctataaaa caagataaaa gagcacttag ttttgtaaaa
    57061 ggagataagg aaaaatacaa ggctctatat gataataatg aaccatttgc aaagagatat
    57121 gttagaaatg taatagaaaa agaaaacaga gcattgatta aaaaaggaga agaaaatgca
    57181 aaaatcaaaa ttgcaggaaa actttatgat agaggcatgt cttttgaaga tatatcagat
    57241 atagttgaga ttgaaataag caagttaaaa gtgagtttag gtgtttttta agggaaatag
    57301 ttaataaaaa aataatcgac tagctctttg taggacagca aaattagtga tataatttga
    57361 tgtataataa attattttta ttagataagg gggaattaaa gttgaagatt acagtattat
    57421 accaaacaag aagtggaaac acagaaaggg tcgctaagct gatagaagag ggcgctaaaa
    57481 aggtggatgg agtagatgta aagcttatga gacttgataa tattgattta gattatttaa
    57541 atgaaagtaa agcagtaata tttggaacac ctacttattt agcaaataca acttgggaag
    57601 taaaaaaatg gtttgatgaa gattcaaaaa aagttaattt agctggaaaa ttaggagctg
    57661 tttttgctac atgtgattat atatgtggag gtccagatgt tgcgatactt actatagtag
    57721 gacatttgat ggttaaaggg atgcttgtgt attctggagg tggttcttta ggaaagccat
    57781 ttatacattt aggacatgta cattcaattg aaggtccaga gttacaagat gagaaggcta
    57841 taatatttgg tgaaagaatt gctactaagg caaaagaact atttgcttaa tttctatatg
    57901 cataataatg attagagtaa atttagggtt ttaatggaga tttgaagtaa aagttataaa
    57961 ataaaaaatt ttccataaaa ttactcaaga tgaattataa tataattata aataaattat
    58021 attattaaaa tatttaaaat caaacgtcaa tagcgaggga agtttatggt atggcatata
    58081 gaatatgaga ttttttctgc aattatagtt atttttttaa tggtatactt ttttagaagt
    58141 aaatttatac ctactttaca aaacaaaata tattgtgcac ttttaatatt ctcattttta
    58201 tttataattt caaatatttt aggttcattt tgtctaaata atatagacaa aattccaatt
    58261 tttattactt ttttattgaa tcagatatac ttattgttat tgccaatacc agcagcactt
    58321 gtatcatttt atgttatggc tattatttat caagatataa ggtatatgaa aaaaagatta
    58381 ttatttttat ctataccact tataatttgt ataatcttat ctattacaaa tccttttacg
    58441 catattctat ttggattgtc aaaagaaaca ggttatatac gaggttgtgg atatgttgca
    58501 acttttgcat cttttttctt ttatgcaata tattcagcat ttttatcttt taaatataga
    58561 aaagcaatgc atgaatcaaa aatatgggct attagaggtt ttcttatagt ttcaattgtg
    58621 gcaattatct tacaggcaat ttttttacag tatcttctta caggaactgc atgtgcatgt
    58681 agtttacttt tgacatatct ttctatgcag aatcgaggtc ttattattga tgaccttact
    58741 ggtgtactca atagacaatc ttttattcaa gagctagatt tgaatatcaa tactgaacaa
    58801 tcaggattta ttattacaat tgcgcttgat gattttaagt ttatgaatga aacttttgga
    58861 acaaaaaatg gagatattgc actaaaagaa gttggaaaat atttgattca gatacatgat
    58921 gtaaaccatg tatatagaat agggggagat atattttcta ttgttgtcaa tagtcaatta
    58981 ggtatagaac cagatgatat tatcagaaag atagaagaaa gatttaaaaa gccttggcta
    59041 gtagaagaga taagttttaa tttagcaact agtattgctg ttgtttatta cccagaaaat
    59101 gcagaaacga cagaagatgt aattgttgct atagatttct ctatacatga agcaaaacgt
    59161 tctgagtcaa ggcgagttgt ttatgcagat gcgtctataa gtgaaaagat aaagaagaaa
    59221 catgttatta aaaattgttt aaaagaagca ttaaaaaatg atgggtttga agtgtactac
    59281 caacctatat tttctaatag caggggcagg tttacttcag cagaggcatt acttaggcta
    59341 gaacacaaag agttaggttt tatacctcct gatgaattta ttccaattgc agaacaaaca
    59401 ggattgatta actcaattgg attagtagtc tttgagaagg tatgtagatt tatagctagt
    59461 gatgagtttg aagatttaaa attggataat atagctgtga atctttctgt agtacaatgt
    59521 atgcaaaaaa acttagctga tgatttactt tatcttatga ataaatataa tgtatcacca
    59581 actaaattta aacttgaaat tactgagact gtggctatgg gctcttttaa tataattaaa
    59641 gaaacaatgg aaagattaat tgatttaggt gttaagtttg cacttgatga ttttggaatt
    59701 gcttattctg gagttactaa tatgctttct ttaccatttt cagtaattaa gttagataag
    59761 agtttgattt ggtctatgaa tgaagatagt aggcataaac ttacagttga gacgattata
    59821 gctcttataa ataaattgaa tatgaaggct gtagcagaag gtgttgaaac cattaagtgt
    59881 gctgaagatt taatagctat ggagtgtgaa tatttgcaag ggtattattt ttctaaacca
    59941 gttccagaag atgtttttaa aaatttgttg aaagaaaata cttttacaag ttaacaccct
    60001 ttattttttt aattacaact ttgttcattg acactgccag cgatatatta taaaattaac
    60061 acaataataa atattaaatt tattatacat attattaata ttaattaatg gagtaaagat
    60121 ttattatata ttttatttaa ttatcctaaa gtgagaaagg aagatgtgac atgaataaaa
    60181 agaaaatatt agtgccaatt gatgggacag aaagaagtat gcattctttg gaatttataa
    60241 aaggaatatt taaaaaagat gaagttgaag tagaaataat gaatgtaaaa gaactagtat
    60301 ttattgatgg catatccctg gctgaagaga taaaaaattc tgaaaattta ggaagaagaa
    60361 tcttggataa agcagcagag ataatgggag agtatgatgt taaagtacat tttacttttg
    60421 gatatccagg agatgagata attagaaaag caaaagaaga taatgtagat tttatagtta
    60481 tgacaaaatc tactaaaaaa ggtcttacaa gaatgatagg ttcagtaaca gcaagtgtag
    60541 ttaaacaagc aaaatgtata gtgatgattg taccggagta gaattagaaa ggtatttgaa
    60601 atatgaataa aaagatagta gcagtttgtg aaagtgaaag aagtttaaat tctcttaaga
    60661 cagctgcaaa tgatttagga tttgatttag tttatgaaat tcaagaaaat gataatataa
    60721 aaaatgaact ttctatttat gatatagaag atgcatccat agtgttattt gttacaagtg
    60781 attctataga aagcatagag aagattgata gatttataga tagagaattt tatgaagttg
    60841 atccaaagta tataatagag gatgcaaaat ctatagtaag tgaaatttta atagacttaa
    60901 attaatcgtg ggtgtcgcac taagaataga gcgacgcctt tttatatatt ttacacaaga
    60961 aataaagagg ttgtatcaaa atatagataa gagtcttttt cattgttgaa atgatgaaaa
    61021 tatatttgat ttcatacaaa aaagagtacc tcataactaa aaagttcact ttgagacact
    61081 ccatttttat atgttagttg catttaaggt aaaatagaag tttaatcatg ttgaaataac
    61141 tgaattacat cttctaaatg attgtacatt gatgtataac ctaagtaaga gtctttacta
    61201 tcaaatgcaa tcattatctt gttatggtgg ctaatcatat ccttgagaga atttctatat
    61261 ttaaaaccaa agtattggtt agaagaaact gttttaggta ttaactcata atattttata
    61321 aaaattggat tatgagaagc taaaattatc tctttgtgaa attgcaagtc agcatcataa
    61381 cattcttcta tcaaaccgtg agaaacctta tccaattctt ctattgactt taggtaagta
    61441 tattttattt tttgaagttc tttagatgta gcatttaaaa ttgctagttt agtagcttca
    61501 ctttcgataa gttgtcttgc tcttaaagat tctacataaa attcttgtat gttatgattt
    61561 ttatagattt caccatcatc aaataggtca acttttttaa atccttcaac tataggactt
    61621 gaataaggca cagctctaag tattccttcc ttacagagta tatttatggc atttcttaca
    61681 ggtattctac ttatgttaaa taggtcagca atctttctct cacttggtaa tttactatta
    61741 attttaaaag ttccatttag aatgtttaat ttaatataat caataatttt ttcagtggca
    61801 tttaagcttt tcataaaaac tcctaaacat ataatttttt tatgattgta ttatatcata
    61861 gttaagagtt aaatctactt ttttataata tgtataagac aggatagagg ccaaaatagc
    61921 tataatagcc aagataaagt atccatattg tattgaagta tattccgata ttttaccaac
    61981 tatcattgga aatacagaca ttcctatagc ataaattgct tgaaaaaaac ccattgcagt
    62041 agatttctga tgttttggta catttttcat agactcgcta gtaaggtaag aaaggagtat
    62101 acctgtagat atacctggta gtacttgtag tacaaatatt atattaatat tttttattgt
    62161 tggaactaaa aagcaatagg ctgaaactaa aataaaaact aaaggaatcc aaaattttgg
    62221 cccatactta gtacaaaatt tagtagaagc aaagccagaa gaagcaacag cacacaccat
    62281 gtaaaatata gaagatagac ctatgagggt agaactagaa cctaagtttt taagaatttg
    62341 agtagtaaat gacattgttg tagacatttg aataccctgt tgaattaatg ccataaagga
    62401 aaaaaataca agtcttttat ccttgcagat agataaatat tttttaattg atattttttc
    62461 aggtattttt tttgaattat gattttttat atttaaggct agaatcgttc caataactga
    62521 agacaataca ctcatgatac ataaggctgt cattcctaat ttttcataaa ataaagtact
    62581 cataacaaat ccaatcaaca ttcctagatt gtttactaat attattgtac ttgtagcttt
    62641 ttgttgatgt tctgtagaaa aaaagttggt atagtgaacc atgtaagaaa tccaagttga
    62701 tgatgatatt ccagataaaa tgttagctat aagaaaacca ttgcctccag gagaaaagac
    62761 tcttataaga gatgctaaac cagcaaagaa aattccagac agtataaata ttttatgttt
    62821 atttatggaa tcagcaaata ctccaacagg aagccttaat aaacattgtg agattccata
    62881 tgcacctatt attataccaa tcatatttgt agatacatta catgcactta aatatggatt
    62941 ttggaaaggt atatatacat attgtgaaaa ccagaaaaat gtaacaatag aaagtaaaag
    63001 taatttttga ttagatattt ttttcatatt atcctccgta tggtattttt ataatgcaaa
    63061 taatatacca tttggatttt ttaatcaagt actttctcta ttaaaaaaaa tttaaatata
    63121 taattattat ttacaaaaaa attatacttt atagttgaat taagaaaacg cttatgatac
    63181 tataaagtta acaaatttga atatataaca gtagacgtgt tactggtaat gcaggcaaga
    63241 tcgaatcaat ttttatagat tatctataga gtactatggg tataatctat ttatttggtt
    63301 cgattttttt gtttaattgg ccataaaaca aaaagcctat agttgaaaat ggaggatttt
    63361 ttatggatta taaaaatata gctcaagaaa ttctactaaa tgtaggagga aaagagaatg
    63421 taaatgaagt gactcattgt atgacaagac ttagatttaa agtcaaaagc gcttctaaag
    63481 tgaataagga taaattatct aaaactgaag gagtaataac tgttgttgag agtatgggtc
    63541 aaatacaagt tgtaattgga aataaagtaa aaaaagtata tgatgaggtt ataaaaattg
    63601 tacctcaatc aaataacgta ggtaaaaaga atgaaagtca agaaaagcaa ggtataatta
    63661 attctatatt atcagcagta gctggtatat ttacacctac aatacctgct atagcagggg
    63721 ttggtatgat taaaggtatt ttatcagttc ttgctatgta ttatatgaat aaaaatggag
    63781 tagatataaa agaaacgcaa agctatatta tcttaaatgc catggctgat tctatattct
    63841 attttatgcc tattatacta ggatatactg cggctaaagt atttaatgca aataaaataa
    63901 tatctatggt attaggagct acactttgtt atccaacttt tactgctttg atggcaggtg
    63961 aagaatctgt tagattttta ggattggcag taacaaaagc tacgtataca tcatctgtta
    64021 ttccaataat tattgctata tgggctcttt catacgttga aaaagtatta gaaaagtata
    64081 tacctgaggt aattaaaata ataatggttc caactttatc attggtagta atgttacctg
    64141 caacattatt tttatttgga ccaatcggaa tttatatagg aaatgtcata aacttttctt
    64201 ataagtatat ttatgaatta agcccagcat tgtgtggagc atttgtaggt gggttatggt
    64261 gtgtgttggt tatatttggt gctcatagag cattattacc aataggtata agtgatgttg
    64321 ctcaaacagg aagacagaat ttattggcat ttgcaggagc tgcaaacttt tcacaagcag
    64381 gggctgctct aggagtattc tttaaaacta aaaatcaagg actaaaaaca atttctatgt
    64441 ctgcaactat aacagcttta tttggtataa ctgaacctgc aatatacggt gccaatctta
    64501 gattaaaaaa gcctatggta tgtgccgtaa tatgtggtgc atttggaggt gcgataatgg
    64561 gaatgggtgg agcttatggt aatgcatttg caaaccaagg tgttttaact atacctgtat
    64621 atgctgaagc aggtgcatta ggtttcttaa gttatctagg tggttgtgct atagcatttt
    64681 ttggttcagc tatttcaact tatctagttg gatttgaaga tttggaagag tcttctaata
    64741 aggaaaattc atctataaaa gtagaaacga aggatggtgt tattgatata acttcacctg
    64801 tagagggcga atgtatagaa ttgtcagaag taaaggatga tgtatttgct tcaaaagcta
    64861 tgggagaagg tatagctgtg ttacctacaa aaggagtaat aacagcccct actgattgtg
    64921 aagtagcttc tttatttcca acattacatg caataggatt aaaattagat aatggagcag
    64981 aaatgttaat acatgttgga atcaatacag tagagttaaa tggtaaatac tttaccaaac
    65041 atgttaatca aggagattta gtgaaaaaag gtgataaact tatatcgttt gatatagata
    65101 aaataaaaaa ggttggttat gatgtaacta cgccagttat agtaaacaat acatttgatt
    65161 ttgggcaagt agtttcttgt aaatctagtt atgtttcaac caatgataat ataatttcat
    65221 tagtattagc tggtaattaa gttaatttga ctagagataa gtaaattgat ttaaaaggaa
    65281 ataagtaaat caacaattaa atacatctaa aagtaagttt attgcaatga attgttaaag
    65341 aaaataatta atatggagta aatctattgc aatgaagtaa caaagaaaat aattaatata
    65401 gagtaagttt attgtaatga attgttaaag aaaataatta atatagggta aatctatttc
    65461 aatgaaataa caaagaaaag agttaatata gagtgaactt attgcaatga atcaacaaag
    65521 aaaataatta ataaaaagga gaaatattta tgaatacagg atttccaaaa gactttttat
    65581 ggggagcatc atcttctgca tttcaagtgg aaggagcttg ggataaagac aataaaggaa
    65641 aaaccgtagc cgactataat tcttttaaaa agtctcattt acaggcagat actaaagtcg
    65701 catcagactt ttaccataat tatgaagaag atattgaatt aatgaaagaa ttagggatga
    65761 aaacatatcg tttttcaatt tcatgggcaa ggataatacc agatggtgaa ggagagataa
    65821 accagaaagg tttagacttt tataataaga taatagacaa actaatagag tgtgatattg
    65881 agccattcgt gactttatac cattttgacc ttccatttaa attagtagag aaatataatg
    65941 gttgggaaag tagagaaact gtctatgctt ttgaaagatt tgctaaaata tgctttaagc
    66001 attttggaga tagggtaaag tattggcaac ctcataatga acagaattta attgttagag
    66061 tggaggagag aattaatata tatgatgaaa cagattcatg gaagatagat aaaatgcgtg
    66121 ctcagatgga ttataatctt tgcttagcac atgcattagc agtcaatgca tgtcatgaga
    66181 tgataaaaga aagtaaaatt ggagcagcag tatcatcatc tgtaacatat cctcttactt
    66241 ctaagccaga agatatatat gcagctagaa tgaatgataa ttttaaagtg tactatatgc
    66301 ttgatatgca tcattatgga gaatatccag ggtattatat gaagtatctt gaaaaaagaa
    66361 atatagttcc tcacatggaa gatggggata aagagatact taaaaaagct aaaatggatt
    66421 ttatagctgt aaattattat aggacaaatt gtgcagaagc tttacctgaa gattcacaac
    66481 atccatttgg attaagagaa ggaactgttg attttagtat gtatggacta tttaaaatgt
    66541 caatgaatcc taacttagag gcttctgagt atggagcagc aatagaccca tcaggtctta
    66601 gagttgcctt aaatgaatat tggcaaagat atcacttacc tgttataata acagaaaatg
    66661 gtctaggagc taaagatata ttagaagatg gaaaaattca tgatgactat cgtatagact
    66721 atttaagaag tcatataaat gcatgtaaac tagctattga agatggtgta gagatgattg
    66781 gatattgtcc atggtcattt acagaccttt taagttcttc tcaaggtttt aataagcgtt
    66841 atggacttgt ttatataaat agaactgacc atgaaatttt agatttaaaa agaatcaaga
    66901 aagatagttt ttactggtat aaagaagtta tagagaacaa tggaatagta aaataaacat
    66961 aaatatatat tgagggtttt agtatgtaca ttacaaaggt attaaataat tcgttgctac
    67021 ttgcaaagga tgataatgat gaagaaataa tattgatggg gaaaggcatt ggccataatt
    67081 ataaggccgg gtctgagtta aatgaaaaag atatagaaaa gatttttgtt cttcatgatg
    67141 aaaatataaa aaagagtatt atacaattgg caacagaaat agatgcagaa tattttggta
    67201 tagctcaaat gataattgca tatggaatag aaaaatataa cttaaaactt atgaaccata
    67261 tatatttggc cttaactgac cacattgcct ttgcagtgag acgttttaaa gaaggtataa
    67321 tgattgaaaa tcactatttg ttcgaaatca aggaatttaa tccaaaagag tatgatatag
    67381 gaaaatatgc tattactgtt tttaaggagg ttcttggttt agacctacca gaagaagaga
    67441 ttggtaatat agcaggccat tttataaatg cacaacaaga taatccttat agtgatagaa
    67501 ataagagaag tgctaaaatt gtaaatgcta ttttgcaaat agttcattat cacttttcta
    67561 ttgtatataa taaagagtca ttttattata ggagatttgt aatgcatcta aaggcctttt
    67621 cacaaagatt tctggctaat gaaccatcaa gagaaaaaat agattttata tatgagcaag
    67681 ttcaaaaaaa ttgtaaggaa gagtatgaat gtgtgaaaaa ggtaggagct tatatgtata
    67741 aggaatactc cagagaatta ccaagacaag aggagttata tttgatgatt catattcata
    67801 agattttagg agaattatgc gaaaatagtt agaatatctt aatatgctag taatattata
    67861 aaaaattaaa aattaactgc actttaaata tgaataattg cattacttaa gggggttgtc
    67921 ttaaaattga tttttttaat taaatttgaa gctcaactcc ttttttaaac ctttatatca
    67981 ttacaacttt aatgtttata cgcctgtact tcagatgtat atctactttt tacatttctc
    68041 aacatgcaaa tctaccattc tgtatatagt aaatgtatta tcatgtttgt tttattcaag
    68101 cacacttata atacgcttga ttttttcaca atgatgaaat atactaatgg gtgttatagg
    68161 tataagaatc gcattacact tgaagaagta agtgataaat gagatataaa tcctcatatg
    68221 ataaactact atttcattga ggaacatatt ctaagtgatg tcaagatact acaatatgac
    68281 taaattccta gtgataaaaa agtctacgaa tgagtgtaga aaggggaact aagatgaata
    68341 aagttttaat tatagatgat gataaagatc tttgtatttt gttaaaaaag agtatttcaa
    68401 ttgaacagat tgaagcaaac tattgccatt ctgggagaga tggactggtt gaactttcaa
    68461 aacaagatta tcagttagta gttctagatg tgatgatgcc aggtatggat ggttttgaaa
    68521 ctatggaaca aatacgaaaa tatagcagta ttcctatctt aatgctgaca tcaaaaagtg
    68581 ataattacag caaagtgcat ggattgcgag ctggtgcaga tgactatcta accaaaccat
    68641 ttgaaattac agaatttact gctcgtgtac tgtctttaat ccgacgttac acccgtttta
    68701 atccaaaaaa tgaggagcca cagcaacttt ctttcaaagg tttgttgatt gaccttgacc
    68761 agcatacagt tactaccaat ggcgaaccta tgttgcttcc acccaaggaa tttggtgtac
    68821 ttctttactg tgcaaggcat cagggtaaga ttttgacaaa acagcagatt tatgaagccg
    68881 tgtggggtga gccatatgta tatgatgaca gcaatatcat ggctattatc agtcgcctaa
    68941 gaaaaaaaat tgaactagac ccatcatctc ccatctattt gcaaacaata aaaggaattg
    69001 ggtatcaatt caacacggag gtatgaaaat ggatgaacaa acgataattc ttttcttact
    69061 cttgctgtcg attggtctac tttttctttc catatttgct ttttccaagc ttgggcatat
    69121 tcacaaacgc ttaaaagata tagaggagat actcgctgat gtacaaattg gagacgaaaa
    69181 tcgtaaaata ctaattaagc catgtgatgt tatggctcca ttggtttatc agcttaatga
    69241 gattgtctac gattatgaaa ataagttact ttctctgaaa aagtcggata aagctagcaa
    69301 acaacttatg acaagtttat ctcatgatgt tcgtacacca ttaacaactc tgattggtta
    69361 tctggatgct gtacacagtg gtatagttat cgaacaagag cgggaggaat accttgaaat
    69421 cgctaggcgt agagcctacg acctcaagga ttacattgat gttctttttg actggtttcg
    69481 gttgaactct gatgaattta cgctgtcaat agaatctgtg gaaattgcag agttgtcacg
    69541 caatattttg aaagactgga ttcctatatt ccatgaaaag aaactgaatt ttgaaattga
    69601 tattccagaa aatcgactaa tggtaaacct cgacccagat ggatattccc gtgtggtcaa
    69661 taatctggtt caaaatgttc ttgctcacag taaggctcgg caaatcaaga ttaccatgag
    69721 tgaggattcc agaatggtat tactccgtgt tgaggatgat ggtgttggga ttgccagaga
    69781 aaatttacca catatttttg agcgactgta taagtgtgat aaaggacgtt cagaaaaagg
    69841 aagtggtctt ggtctatcta ttgtcagtca aatggtggag cgaatgggtg gtcaggtttc
    69901 cgtagaaagt gagattggaa agtataccgt atttacagtg agcttaccat taatataaaa
    69961 ttgtttttcc cccatgtgac atgtatacac agggggattt tttgtaagtt ataaagttgc
    70021 aaggttaata caaggttgtt gcaaggttaa tgtaagattg aggtggtatg ataactttaa
    70081 taaacaaagg aggcttttat tatttatgaa taatttaatt attgaaacaa aaaatctcac
    70141 taagcaatat ggtgctcaaa agagcgtttc taatttgaat attcacgttc agaaaggtcg
    70201 catttacgga ttgctaggaa gaaacggtgc gggtaagacg acaactatga aaatgttatt
    70261 gggcctaaca aaaccaacta ctggagaaat taaaatattt ggtcaggaca ttcacaataa
    70321 cgagaagaag cttcttcctc gaattggatg tttgattgaa tcaccagggt tttatcctaa
    70381 tctgacagga acagaaaatc tcaaaatctt tgcaaggctt agaggaatac caaaaaaaaa
    70441 tgcagtagaa tcagcactgg atgtagtcgg tttgccaaac aatgacaaaa agttattttc
    70501 tcaatactct cttggtatga aacagaggtt ggcgattgct cttgccatta tgcatgaccc
    70561 acaactgttg attttggacg agcctatcaa tggacttgat cctattggta ttgcagaagt
    70621 aagaacgttt attcgtgatc tctgcacaga acgaggtaaa actattttga tttccagtca
    70681 cattctttct gaaattgcct tgttggcaga tgacattggc attattgacc atggtatttt
    70741 gttggaggaa gaaagtcttg cccaattaga agaaaaaaac agtaaataca tacattttgc
    70801 tgtatctgat gcagcacagg cagcaaggct tctggaaacc caacttaacc tgaaaaattt
    70861 caaagtagca gataattaca atattatgct atatgataca gatgtttctg ttgcagaaat
    70921 aaatcgtgct ttcattctaa acaacattga ggtatcagaa tctcatttat gcgaagatac
    70981 tcttgaagat tacttcaaga aagtaacagg gggtgaagga attgcttgat ataatttcat
    71041 gtgaattttc aaaactcaag cggttaaaat ttatactgat ttctatttta ggtgcttgct
    71101 tatttccaat tcccgcaaca attttgattg caaaggacaa cttgcccttt gagcaattgt
    71161 tcaaattagt tgtcaatttt ggatatttct tgctattacc aattgtatta agtattgttg
    71221 ctagccaact attttttata gaaagagata atgatgtatt aaaaaatctt gcaacagtgc
    71281 cagtgccaaa aggaaaactg gctctggcaa aattggctgt gttgctgttt atatcattgt
    71341 tctactctgt tgcagggtta ggagctacta tcattggagg tttcattgtt ggaatagtag
    71401 agggggttgc tgtaaaatta ggaatgagca ttgcttttgg aattatgttt tttgttactg
    71461 tacttcctgt tgtggtactc attgtatatt tcaatcgcag ctatattttt tctattatac
    71521 tttcttttgt atatgctatt ttcaatttta gtatatcctt aaatattatc aattttgaac
    71581 caaataaccc actattaagc gtattacctg ctcctgttat aatgcgttgg tggatggcat
    71641 tttggggaga tcccactggc gaatacacgg tacttagaca gccatatttg ctctcaactc
    71701 ctgcttgtgt aggtatttta tgtttgataa ccattatagc agtcctgctg atttgtatga
    71761 tatacaaaaa gcaggagaat taggaggtat ctatgcttga tataattact acagaatttc
    71821 aaaaaataaa aagatataat attctatgga ttggtgttgt tgctgttctg ttttcagcac
    71881 tattggcagt ttttcaggtt gtaagttcac acggcagtga ccctctaacg tatgagagct
    71941 ttgcaaacgg agttatctgg aataatttca gtcttagttt tccatttggg attacactta
    72001 ttggaggata tcttattaac cgtgaatata cagaccaaac cttaaaaaac atcctaactg
    72061 ttcctatttc attacgcaaa ttgctagtgg gaaaattaat tgcggttgga ggaattactg
    72121 tattgttaag tcttttcagc ttcttatgca catttatttt ggggttgtta ttttgcaata
    72181 tcaatatatc tgttatactc gttgtaaaat ccttagttca gattttgtct gttaatctgt
    72241 attgttatgt tgctgttttg ccgattattg cttattttgg tcgtaaacaa aatgcatttc
    72301 tgacgggtgt agggattgca tttgtatacg gtttttgtgg tgtgtttgtt gctggaagaa
    72361 atctgacaga cttttatcca atcacagcag gtcttggtct agttggatac aacaatggtg
    72421 ctggtactgt ttatcagcca cttatcggtg ctatgacttt gattgtaatt ctaatcttga
    72481 caactattct acttgtattt acacctaatt atgataaagt tatgaccata tctaagaaaa
    72541 aagggataaa gcataaaaag tattctcata aataacataa atattgtttc tattatttaa
    72601 gttgctagac agtgaaaaga aaaaatctgt cgtacagtgg ctatatgctc atgtattcaa
    72661 gaagctacag ttttgatttt tcataactgt agcttctttt tgtatttatt aaatcacgac
    72721 tatcataata cttgcaatag gtatagtaac aagagagagt actgtcatat gtgttttata
    72781 tattacatta agtatattct aaaatatatg ttttaaataa tatatttgaa aataggaata
    72841 tttaaataaa aaaaataata ttatatatag ttgacaaaaa ttggcaaatg atatactata
    72901 taaatataga aatagtaatc attactatta ttaaaaaggg aggaattaat tatgaactta
    72961 aaaggaacta aaacagaaaa gaatttaatg gcaggatttg caggagagtc agaagcaaga
    73021 aataaatata catactacgc ttcaaaagca agaaaagaag gttataatca aatagctgct
    73081 atatttgaag aaactgctaa caatgaaaag gaacatgcta aattatggtt taaactttta
    73141 catgatggta tgccttcaac agaggaaaat ttaaaggatg cagcagcagg tgaaaactat
    73201 gaatggactg atatgtatgc aaaatttgct aaagaagcta gagaagaagg atttgacaag
    73261 atagcttatt tatttgaagc tgttggaaaa atagagaaag agcatgaaga aagatactta
    73321 aagcttttag aaaacttaaa tgaaggaaaa atatttaaaa gagatgaaga agttgtttgg
    73381 caatgtcaaa actgtggtca tgtgtatgta ggtactgaag caccagaaaa atgcccagta
    73441 tgtgaccatc caaaagcata ttttaatata aaagctgaaa attattaaaa attatcatta
    73501 ttaaaaagga atgttaacta tgaaattttc taaacaacga gaactgattt taaatgaaat
    73561 attaaataat ccggttcatc ctactgcgga ttacctatat gaaaacttaa aaaaagataa
    73621 tccgaattta agcttaggaa ctgtgtatag aaatttagct cagctaacgg aacatggctt
    73681 tataagaaag gttagtattc cagggtatcc agatagattt gatggtcgaa tagataatca
    73741 ctatcatatc atatgtgaag tatgtggaga ggtatacgat ttagaatccg aagttcttaa
    73801 taatttacaa gaattaatat ctgaggagac agacataaaa ataacatctt ataacataag
    73861 ttttaaaggg atttgtaata attgtaaaag gtgtagccaa gtaggttagt tttcaaattc
    73921 taaaaataat taatgaggtg agtttatgtg tagtgaacaa aaatttttta tatgtgagac
    73981 ttgtggaaac cttgtaggaa tgatacaaag tggtggtgta cctattttct gttgtggaaa
    74041 acctatgaag gaacttgttc ctaatacaac agatgcagca gttgaaaaac atgtacctgt
    74101 aattgaagtg gatggaaata atgttactgt taaagtaagt agcacaactc atccaatgac
    74161 taaagaacat catatagcat gggtgtatct tatgactgaa caaggtggtc aacgcaaatg
    74221 tctagcagta gatggagaac ctgtagttaa atttgctttg aatgatgatg ataaagtgat
    74281 ttctgcctat gcttactgta atttacatgg attatggaag gctgaactgt aatatttaaa
    74341 taaattgagt caaaaaggag gctataaatg tattttcata caaatagcct cctataaatt
    74401 tatataaatt caagttttaa ttttgtttaa tgtcaatata atataaggag aaaataatat
    74461 gtcaatttat aaatgtagtg tttgtggata tatttatgat gaatctaaga atgataaaac
    74521 atgggatgaa ttaagtgaag attgggaatg ccctgtatgt acaaaaggtc gtagttattt
    74581 tggaaagata agtactgttt actatgaaga agatgaaaag atagcagaag acatagttga
    74641 agatgaatct aaattaaata ctgaaaaaga gggagattta aattacttaa gtacatattt
    74701 aagaagagat gatgaagttg agaaacatat ggatataatt cacgaaatgg ctgttacagg
    74761 aaaatctata atagaaccta tgagaactaa actaccagta atttcttggg atgatatttt
    74821 aattatgggt gctcaattaa atccattacc tttaaatgag catgatgaag taaatacaac
    74881 aacaataata ggaaaaaaag caaagaaacc tatgattata gaaaatccag tttatatttc
    74941 ccacatgtca tttggagctt tgtctaaaga actaaaaata gctttagcaa agggtgcagc
    75001 ccaaaataag acagctatgt gtagtggtga aggaggtata ttaccagaag agaaggaagc
    75061 ttcatacaag tatatttttg agtatgtacc aaataaatat agtgtaactg aagaaaatct
    75121 taaaaattct gatgctatag agataaaaat tggtcaagga acaaaacctg gtatgggagg
    75181 tcatcttcct ggcgaaaaag taactgaaga aatagcaaaa gtaagaaata agcctgtagg
    75241 tcaagatgta ataagtccat cttgttttga ggaaatacaa tccaaagagg atttaaagaa
    75301 acttgtagat gaacttagag aagtttcaga agggcgtcca ataggtgtta aaatttcagc
    75361 aggtcatatt gaaaaagata tggaatttat agcttatgct aaacctgatt ttgtaactat
    75421 tgatggtcgt ggtggagcta ctggagcaag tccaaagttg cttaaagatg caacttcaat
    75481 tccaacaata tttgctcttt atcgtgctag aaaatacata gatacacatg gattagatat
    75541 agatttggtt ataacaggtg ggcttcgtat atctactgat tttgctaagg caattgctat
    75601 gggggctgat gcagttgcta ttgcaagttc tgctcttatg gcagcggcgt gtcaacaata
    75661 tcgtatatgt ggttctggta agtgtcctgt aggagtagct actcaggatg aggaattgag
    75721 aaaaagatta catatagaaa atagtgctaa tagagtggca aactttttaa atgtaagttt
    75781 agaagaactt aagacttttg ctagaatttc tggtcataaa gatatacatg atttaagtgt
    75841 tgatgattta tatactgtta actctgaaat atcaaattat acaaacatac agcatgtata
    75901 gattgaagtt tgaaactatt agattttaga cagctataaa agtaaatgat aaatatattt
    75961 tataaaagag agtgtcttaa aatgaacttt ttagttcata agtcactctt ttttgtatga
    76021 agccaaatat atttatcatt ttagcaatga aaaagaatct tatctcttta ttttgagata
    76081 gactccttct atttatgaaa attaaagata ttaaattgta aaatattctg tgatgtcgta
    76141 ctattaaact aaagattctg tactttaaag taaataaaaa tttaaggagt gattattatg
    76201 gaaagattga agatacaagt tttagtcgac aataatactt atattgatag atactttgtt
    76261 ggagaaccag cagtaagcta ttatattgag attgatggaa ataggatttt gtttgactca
    76321 gggtattcag atgtattcat atcaaacgct gaaaaattaa atatagattt aggaaattta
    76381 actcatgttg ttttttctca tgggcataat gaccatacaa gaggtatcca gtttttagaa
    76441 aataggtatg atttatctac agttgaactt atttcacatc ctaactgttt tatacctaga
    76501 aaacatggag agaaaagcat aggagctcca ttttctgctg aggaaattaa aaatattttt
    76561 atgtataatc ctaaggataa gccatttaat ctaagcaaga attgtgtgtt tttaggtgaa
    76621 ataccttcta taaatgattt tgaaaaaaga gctaaaattg gtaaatgtaa aataggagat
    76681 ttgtgggaag atgattatgt tttagatgat tctgctatag tttgtaaaac tgataaaggt
    76741 atttttattg ttacaggatg ttctcatagt ggaatttgta atatagtaga gtatgctaaa
    76801 aaggtatgtg gagatgatag agttattggt atactaggtg gttttcatct ttttgaattg
    76861 aataataggt tagatagtac aatacaatat ttggataaag aagaaataag aatgttgtac
    76921 ccttgtcact gtgtttcttt taaggtaaaa gcgaagatga atgaaatatt gaatataaat
    76981 gaagttggag tagggctgac cataagtata tagtatttga aagtatataa gatttaagtt
    77041 gaaggttaaa aagatatttt ctatagaaaa tatctttt
//
LOCUS       DAMJWS010000007        71924 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMJWS010000007 DAMJWS010000000
VERSION     DAMJWS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 71924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.007"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            32..1312
                     /locus_tag="PC399_000490"
     CDS             32..1312
                     /locus_tag="PC399_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF2591369.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1575..2966)
                     /locus_tag="PC399_000491"
     CDS             complement(1575..2966)
                     /locus_tag="PC399_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2591370.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2977..4197)
                     /locus_tag="PC399_000492"
     CDS             complement(2977..4197)
                     /locus_tag="PC399_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF2591371.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4211..4999)
                     /locus_tag="PC399_000493"
     CDS             complement(4211..4999)
                     /locus_tag="PC399_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2591372.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5016..6290)
                     /gene="larA"
                     /locus_tag="PC399_000494"
     CDS             complement(5016..6290)
                     /gene="larA"
                     /locus_tag="PC399_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF2591373.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6408..7121)
                     /locus_tag="PC399_000495"
     CDS             complement(6408..7121)
                     /locus_tag="PC399_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2591374.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7246..8505
                     /locus_tag="PC399_000496"
     CDS             7246..8505
                     /locus_tag="PC399_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF2591375.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            8785..9351
                     /locus_tag="PC399_000497"
     CDS             8785..9351
                     /locus_tag="PC399_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2591376.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9552..10757)
                     /locus_tag="PC399_000498"
     CDS             complement(9552..10757)
                     /locus_tag="PC399_000498"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2591377.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10860..10966)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11058..11426)
                     /locus_tag="PC399_000499"
     CDS             complement(11058..11426)
                     /locus_tag="PC399_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF2591378.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11478..12755)
                     /gene="obgE"
                     /locus_tag="PC399_000500"
     CDS             complement(11478..12755)
                     /gene="obgE"
                     /locus_tag="PC399_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF2591379.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12866..13156)
                     /gene="rpmA"
                     /locus_tag="PC399_000501"
     CDS             complement(12866..13156)
                     /gene="rpmA"
                     /locus_tag="PC399_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF2591380.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13170..13487)
                     /locus_tag="PC399_000502"
     CDS             complement(13170..13487)
                     /locus_tag="PC399_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF2591381.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13502..13813)
                     /gene="rplU"
                     /locus_tag="PC399_000503"
     CDS             complement(13502..13813)
                     /gene="rplU"
                     /locus_tag="PC399_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF2591382.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13914..15335)
                     /locus_tag="PC399_000504"
     CDS             complement(13914..15335)
                     /locus_tag="PC399_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF2591383.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15353..16054)
                     /locus_tag="PC399_000505"
     CDS             complement(15353..16054)
                     /locus_tag="PC399_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF2591384.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16056..17900)
                     /locus_tag="PC399_000506"
     CDS             complement(16056..17900)
                     /locus_tag="PC399_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF2591385.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18219..19412
                     /locus_tag="PC399_000507"
     CDS             18219..19412
                     /locus_tag="PC399_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2591386.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19624..20706)
                     /locus_tag="PC399_000508"
     CDS             complement(19624..20706)
                     /locus_tag="PC399_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2591387.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(21270..22061)
                     /locus_tag="PC399_000509"
     CDS             complement(21270..22061)
                     /locus_tag="PC399_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591388.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(22065..22394)
                     /locus_tag="PC399_000510"
     CDS             complement(22065..22394)
                     /locus_tag="PC399_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2591389.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(22879..23292)
                     /locus_tag="PC399_000511"
     CDS             complement(22879..23292)
                     /locus_tag="PC399_000511"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF2591390.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(23333..24463)
                     /gene="rodA"
                     /locus_tag="PC399_000512"
     CDS             complement(23333..24463)
                     /gene="rodA"
                     /locus_tag="PC399_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF2591391.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(24548..24832)
                     /gene="minE"
                     /locus_tag="PC399_000513"
     CDS             complement(24548..24832)
                     /gene="minE"
                     /locus_tag="PC399_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF2591392.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(24850..25647)
                     /gene="minD"
                     /locus_tag="PC399_000514"
     CDS             complement(24850..25647)
                     /gene="minD"
                     /locus_tag="PC399_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF2591393.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(25671..26354)
                     /locus_tag="PC399_000515"
     CDS             complement(25671..26354)
                     /locus_tag="PC399_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF2591394.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(26452..29430)
                     /locus_tag="PC399_000516"
     CDS             complement(26452..29430)
                     /locus_tag="PC399_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF2591395.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(29442..29930)
                     /gene="mreD"
                     /locus_tag="PC399_000517"
     CDS             complement(29442..29930)
                     /gene="mreD"
                     /locus_tag="PC399_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF2591396.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(29945..30838)
                     /gene="mreC"
                     /locus_tag="PC399_000518"
     CDS             complement(29945..30838)
                     /gene="mreC"
                     /locus_tag="PC399_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF2591397.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(30848..31858)
                     /locus_tag="PC399_000519"
     CDS             complement(30848..31858)
                     /locus_tag="PC399_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2591398.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(31979..32638)
                     /gene="radC"
                     /locus_tag="PC399_000520"
     CDS             complement(31979..32638)
                     /gene="radC"
                     /locus_tag="PC399_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF2591399.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(32683..33267)
                     /gene="maf"
                     /locus_tag="PC399_000521"
     CDS             complement(32683..33267)
                     /gene="maf"
                     /locus_tag="PC399_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF2591400.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(33487..34458)
                     /locus_tag="PC399_000522"
     CDS             complement(33487..34458)
                     /locus_tag="PC399_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF2591401.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(34474..35049)
                     /gene="rsxA"
                     /locus_tag="PC399_000523"
     CDS             complement(34474..35049)
                     /gene="rsxA"
                     /locus_tag="PC399_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF2591402.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(35064..35657)
                     /locus_tag="PC399_000524"
     CDS             complement(35064..35657)
                     /locus_tag="PC399_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF2591403.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(35670..36239)
                     /locus_tag="PC399_000525"
     CDS             complement(35670..36239)
                     /locus_tag="PC399_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF2591404.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(36239..37216)
                     /locus_tag="PC399_000526"
     CDS             complement(36239..37216)
                     /locus_tag="PC399_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF2591405.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADISSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(37239..38576)
                     /gene="rsxC"
                     /locus_tag="PC399_000527"
     CDS             complement(37239..38576)
                     /gene="rsxC"
                     /locus_tag="PC399_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF2591406.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(38792..39736)
                     /locus_tag="PC399_000528"
     CDS             complement(38792..39736)
                     /locus_tag="PC399_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591407.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(39847..41121)
                     /locus_tag="PC399_000529"
     CDS             complement(39847..41121)
                     /locus_tag="PC399_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2591408.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(41253..41624)
                     /locus_tag="PC399_000530"
     CDS             complement(41253..41624)
                     /locus_tag="PC399_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2591409.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(41852..42451)
                     /locus_tag="PC399_000531"
     CDS             complement(41852..42451)
                     /locus_tag="PC399_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591410.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(42652..42864)
                     /locus_tag="PC399_000532"
     CDS             complement(42652..42864)
                     /locus_tag="PC399_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF2591411.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(42937..44652)
                     /locus_tag="PC399_000533"
     CDS             complement(42937..44652)
                     /locus_tag="PC399_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2591412.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNITSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(44652..45206)
                     /locus_tag="PC399_000534"
     CDS             complement(44652..45206)
                     /locus_tag="PC399_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2591413.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(45199..45801)
                     /locus_tag="PC399_000535"
     CDS             complement(45199..45801)
                     /locus_tag="PC399_000535"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF2591414.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(45810..48458)
                     /gene="polA"
                     /locus_tag="PC399_000536"
     CDS             complement(45810..48458)
                     /gene="polA"
                     /locus_tag="PC399_000536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF2591415.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            48610..48697
                     /locus_tag="PC399_000537"
     tRNA            48610..48697
                     /locus_tag="PC399_000537"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48644..48646,aa:Leu,seq:caa)
     gene            complement(48884..49327)
                     /locus_tag="PC399_000538"
     CDS             complement(48884..49327)
                     /locus_tag="PC399_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591416.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(49330..49527)
                     /locus_tag="PC399_000539"
     CDS             complement(49330..49527)
                     /locus_tag="PC399_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2591417.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(49719..50585)
                     /locus_tag="PC399_000540"
     CDS             complement(49719..50585)
                     /locus_tag="PC399_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2591418.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(50811..51680)
                     /locus_tag="PC399_000541"
     CDS             complement(50811..51680)
                     /locus_tag="PC399_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2591419.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(52186..52407)
                     /locus_tag="PC399_000542"
     CDS             complement(52186..52407)
                     /locus_tag="PC399_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591420.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(52909..53889)
                     /locus_tag="PC399_000543"
     CDS             complement(52909..53889)
                     /locus_tag="PC399_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591421.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(54053..54925)
                     /locus_tag="PC399_000544"
     CDS             complement(54053..54925)
                     /locus_tag="PC399_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2591422.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(55089..55640)
                     /locus_tag="PC399_000545"
     CDS             complement(55089..55640)
                     /locus_tag="PC399_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2591423.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(55660..56562)
                     /locus_tag="PC399_000546"
     CDS             complement(55660..56562)
                     /locus_tag="PC399_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2591424.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDD
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(56577..58949)
                     /locus_tag="PC399_000547"
     CDS             complement(56577..58949)
                     /locus_tag="PC399_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF2591425.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(59170..59859)
                     /locus_tag="PC399_000548"
     CDS             complement(59170..59859)
                     /locus_tag="PC399_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591426.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVYDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(60100..61479)
                     /locus_tag="PC399_000549"
     CDS             complement(60100..61479)
                     /locus_tag="PC399_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2591427.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENVNKYIRIKGTMTRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(61473..62186)
                     /gene="rgbR"
                     /locus_tag="PC399_000550"
     CDS             complement(61473..62186)
                     /gene="rgbR"
                     /locus_tag="PC399_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF2591428.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(62436..62996)
                     /locus_tag="PC399_000551"
     CDS             complement(62436..62996)
                     /locus_tag="PC399_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF2591429.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(63092..63895)
                     /gene="zupT"
                     /locus_tag="PC399_000552"
     CDS             complement(63092..63895)
                     /gene="zupT"
                     /locus_tag="PC399_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF2591430.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(64046..65209)
                     /locus_tag="PC399_000553"
     CDS             complement(64046..65209)
                     /locus_tag="PC399_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2591431.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(65269..66456)
                     /locus_tag="PC399_000554"
     CDS             complement(65269..66456)
                     /locus_tag="PC399_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591432.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(66819..68519)
                     /locus_tag="PC399_000555"
     CDS             complement(66819..68519)
                     /locus_tag="PC399_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591433.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(68774..69049)
                     /locus_tag="PC399_000556"
     CDS             complement(68774..69049)
                     /locus_tag="PC399_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2591434.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(69168..69740)
                     /gene="mobB"
                     /locus_tag="PC399_000557"
     CDS             complement(69168..69740)
                     /gene="mobB"
                     /locus_tag="PC399_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF2591435.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            complement(69724..70905)
                     /locus_tag="PC399_000558"
     CDS             complement(69724..70905)
                     /locus_tag="PC399_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF2591436.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKFSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(71116..71850)
                     /locus_tag="PC399_000559"
     CDS             complement(71116..71850)
                     /locus_tag="PC399_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591437.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
BASE COUNT        22763 a        13214 c         7587 g        28360 t
ORIGIN      
        1 ataaaataat taaagataaa aggagtattt tatgaacata cttggattaa tagttgaata
       61 taacccattt cataatggac acatatatca tttatctaaa tctatagaaa aaacaaatgc
      121 aacacatact gtagcaataa tgagtggaaa ttttctacaa agaggcgaac cagctttatt
      181 tgataaatac actagagcaa aaattgctgt cgaaaatggt gtagacttag ttatagaatt
      241 accaacttta tttgcttgtc aaagtgctga aattttttct catggtgcta taacaatttt
      301 aaattcacta aactgcataa attctatatg ttttggtagt gaagaaggaa atacagaaat
      361 tctttataat atagctgaaa ttttagcaaa tgaaccaaat gaatttaagt tacttttaaa
      421 aaaatattta gatgatggtc tgttatttcc aacagctaga gctaatgcac tttttgatta
      481 tatacatcaa gacaaaaaaa ataattttat acaaaattat tcaaaggaac aattatcaaa
      541 aacattaaat tcatcaaaca acatactggg tatagaatac ataaaaaacc tcatccattt
      601 aaacagtagt ataaaaccat ttactatctc cagggtccaa tcagagtata attccgaaga
      661 aattgagagt gatatttgtt ctgctactgc tataagaact tcacttaaag aaaatacaga
      721 cctttttcat ttaaaaaatg ttattccaga aaaaacttat aacatcctaa atgaaaaaat
      781 agataatggt tttttaccta tgtttgatga cttcttcttt gatattttaa aaacaattat
      841 acttagagat atggacactt taaaagagta ttttgaagta aatgaaggca tagaaaacag
      901 aatctataaa gatatcttta catctgaatt tctacatgaa ctacaattag caattaaatc
      961 caagcgatac actttaacca aaatcaaacg aactctaaac aatatattgt taggtattaa
     1021 aaaaagcgac atgcaactta taaaaaacat agatactata ccttatataa gaatacttgc
     1081 ttttaatgat aaaggtagag aaattctaaa agagataaaa aataaatctg aaataaaaat
     1141 aataaacaag ttttcaaata tatcattttc attagatgat acgattttta aaacactcat
     1201 aagttatgat ataaaagcta caaatatata taattcaata tattataaaa acaataaaga
     1261 tttagtaaaa ggccctatgg atttttatac tcatccaata tatgtaaagt agttttttaa
     1321 aataactata tttataaaaa tcattttatt aactctttat tatataagat acaatagtct
     1381 tactttattt atgtacacat gcttttagga tgacaacatc aacttaaata ttatgttact
     1441 ttctaaattt aagtaaatat tgccatccta aaaattttac aatttagctt cagatttaaa
     1501 agtaaagttt aaaatattat atttaaatct aatttaatat tttttgtttt gtaaatatta
     1561 ttaaattaat atttctaata tattttccct ggatttaaaa tatttttagg gtcaaaagct
     1621 aactttatat ttttaattaa catcatataa gcatctgaat ttgattcatg taagtactct
     1681 tttttagcat atcctatgcc atgttcacca gaaacttgac cactaagttc tctagatttt
     1741 ttatacatat agtcaaaagt ttcttttaat cttattttcc atgtattatc atccatacca
     1801 tcttttaaaa tatatatgtg tagatttcca tctcctgcat gaccaaaact ttttattctt
     1861 atttttaatt tgtcttgaag ctcgtgagtg tatcttataa actctgctat cttatctctt
     1921 ggaacaacga catcacattc atccatttgt gtcgtagatg cctttatagc ttctaaaaat
     1981 gcacctctgg ctgaccatat agaatcgttt ctttcttgtg tatctgatat aaatacatct
     2041 aatgcaccat tttctaaaca taaattagct actttttcat attctttttc tatatcttca
     2101 gtgctatttc catcaaatgt aagcaaaaga taagcatctg atgtattatc tggaaacttt
     2161 ttacctaaaa attcttctgc tgccaatatc acatctcttt ccataaattc tatagccgtt
     2221 ggtattgatt ttgattttat tattttagga acagtttcta ttgccattga taaatctgga
     2281 aatggtataa gcaaacttat acttttcttt ggtaaaggaa gcaattttaa tatagcttta
     2341 gtaactattc ctaaagtacc ttctgagccc actaataaat cctttatact atatccagaa
     2401 ctatttttaa ccacctttcc acctacattt ataatttctc catttggtag tacaacttca
     2461 agacctctta cataatccct tgtaacgcca tattttactg ctctcatacc acctgcatta
     2521 gtgtttatat tacctgcaat tgtagcactt ttttcacctg ggtcaggtgg gtaaaataaa
     2581 tctctatctt gaacatattg acctattgtc atcaataaaa cacctggttc tactgtcaat
     2641 gttaaatttt cataatcaac ttcaagtatt ttattcattt tacatagatt aatcattatt
     2701 ccaccattta tggcaacagc agcacctaca agaccagtac cttgacctct tggtgtgact
     2761 ggtatattat ttttatatgc gtatttcatt attttagata cttgttcagt ttcaagtaca
     2821 tttactaaaa cttcagggta cttctcaatc cctccaagtt catcatgtga aaaatcttca
     2881 tttatatcac ttccaactaa tacattttct tctccacaag tatctattaa aaaatctata
     2941 tcttttttat cgattaattt atacatttct atccccctat ttagctgctg atgccatata
     3001 tttttgatta ttatctttgt tattttctat tttttcaatc aatttaggaa taatatcata
     3061 aatatcacct attaaagcta aatgtgctac atcaagtata ggcgctttat catctttatt
     3121 tattgctact atatagtcag caccctgcat ccctgcaaca aattgaacag ctccagatac
     3181 accacaagtt attatcaatt taggttttac agttctacca ctaagaccta tttgtttttt
     3241 agcatctatc caacctgctt ctattacagg tcttgttcct gccacttgag cgtttaactt
     3301 atcagctaat ttatatatca tctccatatc ttcttgtttt tttattgctc tacttgccac
     3361 aactatcact tcagcttctt ccagaccaac ttctttattt ttttctttta cactcagtac
     3421 cttaatttta gattttagtt cttttttttc tactttacat aaagttatct ttcctgtagt
     3481 attctctatt ttttctggtg ctgaaaatat tttatatctt acagtggcaa attgtggtct
     3541 attattaggt gtgtttatat gagccattat atttccacca aatgctggtc ttatttggtc
     3601 taaatcagta tttgattgaa tatcaagtat tgtacaatct gctgtaagac ctgttctaaa
     3661 tctagcggca agtcttggag ctaatgacct acctaatgta gttccaccaa ctaatactat
     3721 cgttggtttt attttatcta taaagttttc tattgctttt gaatatggtt ctatcctaaa
     3781 atctttaaat tcatcttcat catatacaaa tacttcatcc accccataag aaatcaaatt
     3841 actgcactta tcttttatgt cttttcctac aaatacacaa tacacaggtt gttttatttt
     3901 tgatgccatc tcacgagctt tacctataag ctcgtacgtt acaggatgta tatttccatt
     3961 atgatgttca acatatactg caattcccct ccattcatct ttattaattt gtactttact
     4021 actctctata aattcaaatg cgccttttgg tccacttttg acacacaact tgcacatctt
     4081 acagccagca cttatttcta ctatacctga cttttcttct atagccccaa aaggacatat
     4141 ttctaaaatt ctctttaagt cagttatttt atcttgattt acaactatct ttgacattta
     4201 ttactccccc ttacaccaac tttaattcct ctaatttatt tacaagtgca aaacttagtt
     4261 catctgcatt tcctcttaca atatttttat ctgtatttgg ctttggagga aatattctta
     4321 caacttgagt aggagaacca ttaagtccat agttattttc atttttatct tcaaagtcat
     4381 ctaagctata tacaggtatt tctctatctt tagtattaag ttttaattta aatgaaggta
     4441 gtcttggttg aaaaatgtct ttttctacag ttattaaaca tggatattca atctcacata
     4501 cttgtaaatc atttggcata tctatttcaa caactatact ttcatcttta acttcaatta
     4561 attttgtaac gttagtaaca tgaggtatat ctaaaaattc agctacttca ggtcccacct
     4621 gtgctgtatc accatcagta gtttgttttc cacaaattat taggtctacc tttcccatct
     4681 tcttaatacc ttgtgatatg gtataggaag tagccaatac atctgctcct ccaaatctcc
     4741 tatcacttat taatgctcct tcatcagctc ccatcataaa actttcttca attaccttct
     4801 ttgcctgagg aggacccatg ctaagtactt ttagattcgc tttcttatct tcctttattc
     4861 taagagctgt ttctaaagca tataggtcat agggattcat ttttgaatct actccatctc
     4921 tttttagtac tcctgtagtt tcatcaactt ctactgaagt cgttccagga acttgtttaa
     4981 tacatgtcaa aatttccata aagtacactc cttttttatt taactataac tgatactcca
     5041 tcaggtataa ctactacttt gctctcctta ccaactatac tattcgccat ttctaaagct
     5101 tctttaaagt taaatgcatg tttcatatgc atttttttta tcatttcagg attacactta
     5161 tctgttacca ttatcacagt atatttagat aatattctcg ctagtatctg aaattcccat
     5221 tggtctggaa tcgtatctac tctatcgaca cttctaattt tatctaaaag ttgttttgga
     5281 gaatctgcat ttgctacatt ttcataaaaa gattgacctc catgaccatc attacatgct
     5341 gaaatcatta ttattactcc accgtcttta caagtagctt cagctgctgt cattccttta
     5401 acagattgat aaatattttg gtctagtgga tatccaccat ttgtagttac aacaatatca
     5461 gattttatag aatttacttt tgataactct gttacaaatc tacatccttt agtatgtgct
     5521 ttttcgacat caccagcaaa ggaagcaatt atttttttat ctttatctat tacaacattc
     5581 aatatgaatg ctaattttgc aactttagct gcatatacca tatcctcatg aataggatta
     5641 ttattcaatt ttccagttct tgaattatca ctatctataa agtcactaca gtgattgtac
     5701 attatagtct ttgcagatgc gattcctgga agtacgcttt ttctgccacc tgaaaaacct
     5761 gcaaagaaat gaggttctat aaaaccttct gctatcaaaa gttctgcctc atatgctagt
     5821 ttgtttatat ataaatctcc acctgaaggc aatatcccta ctttacacat agatttatca
     5881 tcagtagata tatgcataac tatatcttca ttatctacta tttcttctcc cattttataa
     5941 attagctctt cccttgtact tggcctatga aatccagttg ccactataat cttcacatca
     6001 atttctggat taacattcct aatccttctt aatataattg gcatagttat tctacttggt
     6061 acaggtcttg tatggtcact tgtaattatg actacattct ttttcccctt aaccagttct
     6121 tccagagaat tagagcctat aggattattc atagattctt caactatctc ttcttgagtc
     6181 ttatgttcta tataattatc cgattttgac tccaaaacac cgagaaaacg ttcatcaggt
     6241 atttctaaaa tcattccttg ttttgaatac ggtactttta actttaccat ttttaatctc
     6301 ccattataat aaactataaa attggtatgt ggtcatacca gttttacatt tatataatag
     6361 taccattatc taccatttgc aagaataatt tattttataa caaactttta attttcattt
     6421 tttctataat ttttacttat aaaatccata tgttcctttg ctttctcaca tgcaagttct
     6481 acatcatttt ctacaactgc attgtatata gcttcatgct gtttaagcaa tatattttca
     6541 ttaaaaggtt ctgcgattat tttttctctt gcatcttcta taaatccatc aaataatttt
     6601 gatgctgtca taaaaagact ttgaattaaa taatttttac ttaatgttgc tagtttactg
     6661 tgaaattttt ggtctaataa taccagtact cgattattat cttttttatt agtttctacc
     6721 ctcatttcat ctattatatg tttcaattct ataacttcag aagaagttat tctctttgaa
     6781 gcaagttttg caatctcaat ttctaggatt attctaagct ctaatatatc ttcaagtgta
     6841 ccattattaa gtttaaaaat catagataaa ggttctatta aagttttttc tatatttgta
     6901 catataaaat ttccttcacc ctgtctgctt tctatcactc ccatagtctc taatacccta
     6961 atagcttccc ttatggaagt tctactcaca ttcatttgtt cagacagttc tctttctgat
     7021 agtaatttat cccccttttt taactctcca tttagtattt tatattgtat ttgctcaatt
     7081 acttgttcat aaactttttt actagctata ctattaaaca ttttttcctc caatatgata
     7141 atcgttgttg taatatatat ctattatata tcttaagcca ttacattcat atatttttat
     7201 tacttttaat ataatatctt ataactcaaa tttacaggtg atattatgaa aaaaaataaa
     7261 attattgctt tttttgctcc atctatcata gttggagttc tagttttagg gattatccta
     7321 tacccaaatg aagctataaa atctgcaaga gaaggctttt ctatatggtc taatgtttta
     7381 gttccatcac tactaccatt tataattgga gcaaacttaa ttgttgattt aaaaatagtg
     7441 gatattatag ggtttatcat caatcccata actagatttg tatttaatgt ttcaggaaag
     7501 agcgctttag cttttgctat atctacagta tctggttatc cagtaggagt aagtttagct
     7561 tctgaattta ggagtaatgg acaaatttct aagtatgaag cacaaagact tgtgtctttt
     7621 tgttctacat caggaccttt attcataata ggttcggttg gtacaggtat gttccaaaat
     7681 tcaaacttag gttatttaat gattctctgc cattatctag gaactatatt ggttggtctt
     7741 ttctttagaa attatggtaa tgaaaacctt ccaaagacaa aattggattt aaaaacaaat
     7801 ataaaaaatg taatcaacat tagaaattca agtgggaatg gtttttttgt tttatttgga
     7861 aatgctgtat ttaatggagt aaacacatta cttacagttg gtggatttgt aattgttttt
     7921 tcagtagtat ttaaaatact atctcttttt aatgtaatct cattaatagc atcagttata
     7981 tatttgcctc tttcattttt aggtgttagt aaagaactct gtcaagcatt tgtaagtggt
     8041 ctatttgaaa ttactatagg atgcaataaa gtatctagta taacaagttc tccagaaatt
     8101 ttaagagcat ctttagctag ttttttgata ggatttagtg gattatccat cctagctcaa
     8161 tgttgtacat ttctatcaaa aacggacata gatttaaaat tatacatact aagtaaattt
     8221 gcgcatggag ttttagctgc tatctttata tttatacttt atccaattgc aaactctgct
     8281 gtgttggttt ctagctttgc tgatacatac aacgttatat ataataattt aatctggttt
     8341 tattacctta gctattatga tacaattctt caaattgtag taataattta tctaatctca
     8401 gccgtattta ttgcaaaaaa acatatgaaa actttatcta caaatgggaa actaatgcga
     8461 tataaaaata cttttttcac taagaaattt tcacgaaaaa aataaaacta aaaataaaac
     8521 taataaaact taactaaata cttccatttt tttattatat ttgtcataat tttaataacc
     8581 tttttaatta gtttaacagc atagttattt catcacaatt taataatttt attagaaata
     8641 tttttcaaaa aattctcctt aatcacatat atcacacttg tataagttta ctgggaaatt
     8701 tttccattat ataatatttc atttatatta tataataaat accgacctaa acttaccaaa
     8761 tcaaactaag aggagtggtt gaaaatggca acaagaccta tagaaataga cgaatataaa
     8821 aagataatgg aactattaca tactggcttt acttatagtg aaaatggcgt tgaaaaaaga
     8881 tttagaaaaa atcctaaagt tgcattggca ctaatgttag aagctaattt aggtctaaga
     8941 atatcagata ttttaagatt aaagataggt aattttaaag gtaatatgtt ggaaataaat
     9001 gaaaaaaaga cgggcaaatt acagtataga ccagttaata aaaatataat tgagtccata
     9061 aatgaacatg caagaaagta taatcttaaa tcaaatgact acttagtaaa tataaagaca
     9121 aaggctattc aaaaacagtt gaggataatt tgtaaacact taaatctgta taatataagt
     9181 acacatagct ttagaaagct ctatgctact actcaatttg aaaagagtaa caataattta
     9241 gagttggtta aagaactttt aaatcacagc tctgtagcca ctacacagcg atatataagg
     9301 gtaactcagc aagctattaa tgaagctagt gaaaatttct ttattggtta attataacgt
     9361 atacattttt ctattatctt attaattcac cagatattaa aaaagagtgt ctcaaaatga
     9421 actttttagt tcataagaca ctcttttttt ataaaatcaa atatattttt attatttcaa
     9481 cactggaaaa aaggcttatc tctctatttt gagacaccct attaaatttt cataattctt
     9541 gcataaacat attaatctat cttcaataat tttttagctg attcactgta attatttctt
     9601 tttacaattt ccaaaactct atcaatctca tgttctgact ttgcacttac aaaaatcgcc
     9661 aattcttcat catctataat atcataacta tctacatatt tatctaaaca ttctaaaatt
     9721 ccatctttat tattataccc accaaatcta actactgcac tacactcatc ttctataagt
     9781 ccacttaaat ccaatttccc ttttgaaaaa gactttatat ttgaacttct attatctatt
     9841 atatctataa tatctgcata aacagcactt cctgttgcac ctcttccagc tcccttacct
     9901 acaaaagaaa gttcacctac tgaatcacct actaaaataa ctacgttgaa ctcattatct
     9961 atctttgaaa gcatattatt attgtccact aatgctggtc taacaaatcc acttacttct
    10021 cctgattcat acttacttct agcaaccaat tttatcttac aatttaattt ttttgcatat
    10081 tctatatctt tttcatctat tgaagttatt ccttctaata ataaatcttt ccaatatact
    10141 atttttccat aagataaagt agacaatata gataactttc tagcactatc atatcctaaa
    10201 acatctgcat ctggattaac ttctgcaaat cccaactctt gtgcctgttt taaagcgaca
    10261 tcatacggta aattttcatc atacatcttt gataatataa aattacaagt cccatttaaa
    10321 atagcgttta tgctctttat attgttgcct tccagtgatt ctatcaatgg ttttaaaact
    10381 ggaataccac ctgccacaga tgcttcaaac ttgatgctaa cattatttaa gttagctaat
    10441 tctataaggt cactaccttt ttcagccagt aaatctttat tagctgtcac tacatgtatt
    10501 tttttattta acgctcttgt tacataatca aaagctggat ttaatcctcc aattacttca
    10561 actagtatat caatatcctt gtcaaatata tcttctatat cagtagttat cttatcaaag
    10621 tacttcttat acttgtgttt ttctttattt ttaactaaaa ttccaacaac ttctatatct
    10681 ctttttgaag tattattttc tattaaatca attaatccac ttcctactgt tccataccct
    10741 aatattgcta tcttcactgt aacatacccc catcattttg attatattaa attatttata
    10801 tatataaata taccaataaa aaaagcttga gtataaactc aagccatatg acgggtttat
    10861 actcatcttt cagcataaat gctgcaggat ttagcaccaa tgtataatta aatatactgg
    10921 ttgccgggct tcacagggcc agtccctctg ccactcttga taagattgct aatttaaatt
    10981 tatgtttaat agtataatac aatttcttcc aattgtcact atttttttaa cttatttccc
    11041 taaaaggata taaactatta tctaacagct cttttagttg gtttaccctt ttttgtaaca
    11101 ttattatttt tagtctttgc tttcgcctgt tcatgtcctg gaaaaagtat tttaggctct
    11161 tcaatattcc tcttgtaaat agtaaattta aaaccaatag cctgcacaaa ttcagctctt
    11221 aaagcttcac atactgcatt agcagtttct ttagtatcaa gacctgcatt ttctaatata
    11281 gtaactttta ctaattctct agttcttaac ataccatcta attgctctaa gaaactctct
    11341 gtaacacctt ctttaccaat ttgtgtagta ggctttaaag tatttgccaa agatcttaaa
    11401 tacgcccttt gttttccttt taacatattc tcacctctat aattctatac ttaaattatc
    11461 tataatctat atttaaatta attgtaaaac tcaaattcta attcataaat tttaaccaca
    11521 tctccatctt ctattcccat ttctctaagc ctatcaaaaa caccttgact ctccatagct
    11581 ttttggaaat attgaaggga ttccatatca tcaaaattta cagaatacat tattcttcta
    11641 agtgctttac ctgtaactac atacacacca tcttcaatat ctatacttaa ctcttcttca
    11701 gtaccaatat caagttctgg tctatacatt tcttcttctg aaactagctc cacatcttct
    11761 acttctttta gcattttaga aacataggct attacttcat ctatgccttg acgagttgct
    11821 gctgacattt taaatactgt atatcctctg ccttctaatt cactcttaaa cttctcaaac
    11881 ttactctcat cttctaatat atcaaattta tttgcaacta caacttgtgg tctctttgaa
    11941 agcttttcat tgtacaattt aagctcatca tttatcttgt caaaatcttc aatagggtct
    12001 cttccctcta atccagatat atctacaata tgtatcaata cctttgttct ctcaacatgt
    12061 cttaagaact catgacctaa tcctatgcct tcagaagcac cctctataat tccaggtata
    12121 tctgcaagta caaaactatc tccaaactta gtttgaacaa cacctagatt tggtgtaagt
    12181 gttgtaaaat gatagtttgc aattttgggt ttagctttag ttacaactga tagaaatgtt
    12241 gatttaccta catttggaaa tcctaaaaga ccaacatctg ctatcatttt aagttcaaga
    12301 gttatccatc tttcttctcc atctgtacca gattttgcaa aagctggagc ttgtcttact
    12361 gcatttgcaa agtgttggtt acctttacca cctataccac cttttgcaac tatagctgta
    12421 tctccttctt tttttaaatc tgctaataca agacctgtag cctcatctct tattactgta
    12481 cctgctggta cttttaatac taaattttct ccatttttac cagctctttt tcctttgctt
    12541 ccgtcgccac cattttgagc ttgatatttt ttctggtact taaagtccat tagagttcta
    12601 agccccaaat caacttcaaa tatgatgctt gctccacgac caccatctcc accgtctgga
    12661 ccaccagctg gaacgtattt ttctcttcta aatgctaccg aaccatttcc accatttcca
    12721 gccttaacaa atattcttgc cttatctata aacaaattct tcacctactt aattaaaaac
    12781 ttttaattag cattaccaaa aataccatta taaattcatc agtattataa gaaaaaggag
    12841 tgcctcaaca ctccttttat gtatattact cagctatagc tactggatat atactaactt
    12901 tttttctctt cttgtctttt ctttcgaatt taacagtacc atcaactaat gcaaataaag
    12961 tatcatcact accttttcca acattagttc ctggatgtat tttagttcct ctttgtctaa
    13021 ctattatact tccagcagtt actaattgac catcgaatct cttaacacct aatcttttag
    13081 atatagaatc tctaccattt ttagaagaac ctaccccttt tttagatgct aataattgta
    13141 aattcatgtt taacatatgg agtcaccccc tactttttta aaatttttat attttttggg
    13201 tattcccgtt taatttcttt aaccgctaat tgaaagtgat ttagtaataa ttgtgcgtct
    13261 tttacatgtg tatctttgac tatacactct atatgacctt cttctcctac tactttttca
    13321 aattcaatct tttgtaattt gtctaaagaa tttataactg caatagcatt agatgtaact
    13381 gcagcacata ctatatcata gcctatttca gcaaaatcag catgtccctt taaacaaaaa
    13441 ccttgcaaca agaaatcatc attataataa tatttaactt ttatcataac ttaaccaata
    13501 actaagcgtt tattttttct acaactatct tagtgtatga ttgacgatgt ccttgttttc
    13561 ttctgtaatc tttttttgct ttatacttga aaactataac tttcttagct ttaccttgtt
    13621 caacaacttt agcttgaaca gatgctcctt ctactactgg agaacctaat tttaattctc
    13681 catctttaga gcaagctaat acttcgttta aagtaacaac atcacctgcg ttagcttcta
    13741 atttttcaac gaataataca tcaccttcag aaactttata ttgctttcca ccagtcttta
    13801 ctatagcgta cattaaatac acctcctcgg cctcaaactc gccagtacaa ggtacttcat
    13861 ttgaagattt aaacctttac tgtgcggcat tacaattatt aattataaaa tatttacatt
    13921 ttattgtcaa ctattttatc gaaaataaca ttggttttat tgtgctttaa tgtactattt
    13981 gcgtttaaag aaatctttat attatatttt tcaccaattt tatcaattat atccatataa
    14041 tattctttta tttgttcaaa aataaaatca ttaaattcta ccattatgtt tttgtaaaca
    14101 gtatgttcac ttattctcat aatttctttt tcaatactat ctagtatata atgtacagat
    14161 tttattgttt gttcaccatc acaacatgga caagacatta aataatactt atcaatggat
    14221 tctttttctc tacgcctagc aacttctact aaaccaagtt ttgtcatacc taatacttca
    14281 gcttttcttt tatctttatt aaattcttct tctaggatgt gtaaaatctc cttttgatgc
    14341 ttctttttat gcatatcaat aaaatcgatt attattatcc cagctatgtc tctaattcta
    14401 agctgtctta caatttcttt tgcagcttca agattagttt tataaacggt ttcatcaagt
    14461 ttaccagttc caataaactt acccgtattt acatctataa ccgtaagagc ttctgttttt
    14521 tcaattatta aataacctcc actctttaac catacttttt tatttaataa tttttctatt
    14581 ttagattcta ttctgtataa atcaaataca tctctatttt cctcaagaca cagtttatca
    14641 acataagtct tatctatacc tcttaaaata gacttaagtt cagagtactt atcataacta
    14701 ttggtaatta tagattcaat atcatcattc acattatctt ttacatattt agttgcaaaa
    14761 tctaaagact tatataataa tttcggacct ataccaagtt tatattcttt taaaatgttt
    14821 tcatacttta atttcaactc ttctatatct ttctttattt catcatgagt gcaaccttga
    14881 gcctcagtcc ttattattaa tcctagattt tccttattta ctgccttagt aatttttttt
    14941 aatctaaatc gttctttttc atcagtaatt ttatttgata ttgtagttct atcattggaa
    15001 ggtatataga caatatacct tccagaaata ctaatctcag tgtttaattt tgcgccttta
    15061 gttcctattt cttctttatt tatctgaacc aaaatatcct gaccagattt tatagtactt
    15121 ccctttttta attgtaaata agcaagtttc ttaaatccaa tatctacaaa acaagcttct
    15181 attcctggta taactttttt tacgattcca cgatatatat tggatacagt cttcttattt
    15241 aaattgtctt caacaaaaag ttctgttaat ctttcatctt caagtactgc tgttttctgg
    15301 gaccctatta gggactcaat aactatcttt ttcacaactt cacctctcga gcttacatag
    15361 gagtcactaa ttccccatct tctaatacat ataaatctct tctcaatata tcaacatcta
    15421 atgggtcaat atcaagttca aacatctcta atatctttgg tataagtata ttagtattta
    15481 aattagtttt tgagcctgta gctatagtag cagtaagagt aatatgttca tcttctaaat
    15541 ttaaaacatc aaaggttctt atcataggtc ttatatctac ttcaaccatc ttaccttttt
    15601 tattcttttt tgttatgatt atttctttat tattcataaa gtcaattact tttcttttta
    15661 caaactcttt cgtcataggt cttttcaaat ctatattaaa caaatattct ccatagtcta
    15721 tcacagaaga aagagaaggt gtttgtctat caatttcttt tgctttgata aaatctatcc
    15781 catctggaag ttgaattgat actttattta aaaattcctc tactgataaa tcatcttctt
    15841 ctatttcaat atctacatac tcaccttgac tttctgtccc taaagccaaa gcattgccat
    15901 aactcatttt agggtgagga ttaaatcctt gagaatgtga taagtttatt tcagctcttc
    15961 taaacgctct ttgtaacaat ctctgaaggt caagatgtga tatatatatc atgtcgcctt
    16021 cctttttaaa ttttactctt attatcttac tcatattagc aaagccccct tcctatatca
    16081 tgtatattta ttccacaacc attgcagtta tgtctacaat cagaagtgat tttttctttt
    16141 ttagcatttt cgtcttctct aattaagaat tttttactaa tacctacatc tatatggtcc
    16201 caaggaaata cctcttcata gtttctattt ctatgtgcat aaaaatctat agaaagatta
    16261 ttcttttcca tagcttcttt ccatatatct aaattaaaat gttctgccca tccatcaaat
    16321 ttagcaccta gtctaaatgc atcatatata acttttccta ttcttctatc gcctctagct
    16381 actactgctt ccatcaaact agtctttgaa tcatgataat tgtattttat attgttattt
    16441 cttaatttat ttactaaatg tctctgctta ttaataactt cttctgtagt atcttgacca
    16501 tgccattgga aaggtgtaaa tggctttggt acaaatgtag aagtactcac tgtaacacta
    16561 aaacttctct ttagctttcc tccatttact tttctataaa tatcaataac tttataagct
    16621 aatttagcta taccatctaa atcttcataa gtctcagttg gtaaaccaat cataaaatac
    16681 aacttaacac tattccaccc catctcaaaa gctctttctg ttgcattttc caaatcttct
    16741 tctgtaacac ctttatttat aacatctcta agtctttgag ttcctgcttc tggagcaaaa
    16801 gtaagtccag attttcttac ttgttgtatt ttatcagcaa tttccattga aaaattatct
    16861 aatctaaggg atggaagcga aatcccaata ttattagatg catattcatc taccaaataa
    16921 tcagtcaact ctgataactt actataatca ctcgtactaa gtgatgataa tgatatttca
    16981 tcatatccag tattcttaac taatttatct aatatttctt taagtctttt aacactcttt
    17041 tctcttattg gtctgtatat catacctgct tgacagaatc tacatcctct agtacatcct
    17101 ctaaataatt ctagtactat tctatcatga acagtatcta tataaggtac tattagtttt
    17161 tctggataat ctacagtctc aacatctttt attatccttt tagtaggatt tttagtaatc
    17221 ccttctctat taggagttac actttgaact gtaccatctt cattgtactt gacatcataa
    17281 aaacttggta tgtatactcc ttctatgcta gatattttat ataaaaaatc ttctctagtc
    17341 gttttatttt tcttccattc tttatactca tttactactt ctaaattaac ctcttcacct
    17401 tcacctaata ttactacatc tacaaaatct gctaatggtt cggaattata agcacaagga
    17461 ccaccaacca ttataaatgg gtcttccaaa gttctttctt cttttaatat tggtatattg
    17521 gctaaatcta atatatttaa gatattagtg tatgaaagtt catattgaag tgtaaaagtg
    17581 acaaaatcaa agtttgttat aggctctctt gactctaatg caaataatgg tatacttttt
    17641 tctctcatta tattttccat atcaactgca ggagaataca ctctctcaca aaatacatct
    17701 tcatccttat ttattacatc atataatata tgactcccta agtgactcat accaacttca
    17761 tataaatctg gaaagcaatg tgcatatctt atcaattcac tattactagt atctttatga
    17821 attgaattta tttcatttcc aagatatcta gcaggctttt ctactttttt taagattttt
    17881 ttcaagtcta ctttattcat ttattaaaat tcctccaaac aagctcttta tttttattct
    17941 tatttataag taatattata cacgataaaa tattttctat caaaaatata actaaaatat
    18001 tgaatttaaa atacatatta ttcacaaaat agaatagcta aaaatattta agaatatttt
    18061 ctattaaatt tttattgaaa tacaattttt ttacaaagta tgatatatta taaacataaa
    18121 actattgttt tacatgactt atcatgacat cttttgtttc attcacctca aaatggggta
    18181 tgttacttat ataatgataa attttaggag ggttaaaaat gagtaaagtt tttgaagtaa
    18241 aaaaagatat ctactttact ggagtagtag atgaaggttt aaaagtattc gacataataa
    18301 tggaaactga gtttgggaca acttacaact cttatttaat caaggacgaa aaaacggttt
    18361 tatttgacac tgtaaaggca aattttaaag atgagttttt aagtaattta tcagaggtta
    18421 ctgatatagc taaaattgac tatgttgtaa ttcatcacac agaaccagac catgcaggta
    18481 gtttaaaata cttattagat ataaatccta atatagaagt ttattgtact aaagctgcta
    18541 aactatactt agatggtcaa ataaatagac cttttaactg tcatgtaata aaagatggtg
    18601 aaattttaaa tatcggtaaa agaaatctta gatttataac tgctccattc ttacattggg
    18661 ttgatacaat gtttacttac attgaggagg acaagactct tttaacttgt gatgcatttg
    18721 gttgtcactt tgcaagtgtt gatgcagagg tagtgaatag tgaagattat ctaaaatcag
    18781 ctaaacacta ctatgattgt atagttaagc catttgctaa acatgtttta agtgctgtag
    18841 ataaggtggt aggactaaac atagaatttg atactatatt gacttctcat ggtccaatgc
    18901 ttactaaaga cccaatggct gctgttaaaa gatatgttga gtggtctaca gaagctgtta
    18961 atacaactaa tcaaaatcaa gtttctatat tctacttatc agcttatagt aatactttag
    19021 agatggctaa aaagataaaa gaaggacttg ataaagaagg tgcaaaggct gaattatatg
    19081 atttagaaga tatgacatta acagaaatgc acgatacatt agtagtgtca aaagtaatat
    19141 tactaggttc tccaactata aacaaaacta tggttaaacc tatgtgggat ttattctctg
    19201 ttatagaccc aatggctaac caaggcaaga tagcaggagt atttggttca tttggttgga
    19261 gtggtgaagg tataactatg gctgagactt tacttaaatc tatgtcattc aaaatgcctg
    19321 tagaatctct taagaaaaaa ttcttcccta gtgaagagac attaaaagag tgtatggcat
    19381 ttggtgcaga atttgcaaaa ttagttaaat agccattaat caaacataat attatttaaa
    19441 ataaatgatt ttatatttaa aaataaatta aaatggagtt gtctcaaaat taatttttct
    19501 aatctaattt taagttcaac tcctttttta ttttctaaaa tgactgatat aatgataaat
    19561 gcaccctttg tgcgagggtg cattatttat aatatgttct atttgtacac tagtatattt
    19621 gtattaattc acatatctag cgtatgctac catccctctt ccagaattag atgccgtttc
    19681 accaatttcc actttaataa ttacattatt ttctttacac ttttttgata attctgctgc
    19741 agaaaggttc ttaccagtaa aatctaaaga aaatggctta tcatattcaa aaggatactc
    19801 atcaggtaca ccatctggag ctaatgcaac cataccatat ggtgtattca taaaccctat
    19861 tacagtaact gtatgctttg atgggtctaa atttgaattt ttgcttgtaa acttaattgt
    19921 atttattgat gagttgatta aactacacgg aactccctca tacatttcaa tttttggacg
    19981 tagattattt ccataaactt catcaagtat tgccccttgt tgatctgcta tcatagtgtg
    20041 attcccaggt ccaactggcg cagaaggctt agctggcgta ggtttcttaa cagttggcgt
    20101 tggattctgt gtctttacta cttcgccaac agctggatat gctttcttgt caaatggtaa
    20161 tgggtgagta agactcttgt atatgatgtc tgctcctgct gatttgttgg catcaatatc
    20221 tacatgatac aaacgtccat cctcaaaacg aataagattc cactgatggt catttgttgt
    20281 actatgtaca taataacatt caattcccat agcatcacat aaagctttca agctcctaca
    20341 cattccagaa caagcacctt ttttagtaac caatgtataa gcagtattgg ctccctctgc
    20401 tgcgttccag ttaacatatg ttgtgttgtt tttaatgtga ttataagcaa ccctaatctt
    20461 agcatctgtg gtcatgttct ttgtaatttt cttgttcata aagtctgctg ttgcttgtgc
    20521 taccgaatta agttgagctt gtgataactt tggaccatat atactgttgg ttccagctac
    20581 ataacttttc ggtgtctttg cagtaacatc tttagctgtc ataccataaa ctgttacact
    20641 catacatgag gttaaaacca tcattgcaat caatagttta gtagctaatt tcctgatttt
    20701 tttcatatta gcattctcct ttataatata ttttactttc aattccctac cattaattaa
    20761 gttcatttta gatttaaaat atggattatc aacatgtatg catagcaatt cacgtggtca
    20821 tagaatattt agatttcttg gaaaatctgt tctatatttc ctatatagtt cgtatacaat
    20881 ccaactcaca aacctgcatt tttgatatca tcacaataaa atgataataa ttaatatatt
    20941 caaaaatgtt ggtctgtaac aggtatgtca attttccaat tattggtaat ggcgagagtg
    21001 aggagaattg tcaataatac atttaaaatt aaaattgtat acacttcata cattctcccc
    21061 taacaacatt atacagatta taatttgtat attcaataca ttttttggaa agtataatta
    21121 tatcaaaata tgtgattatg tatagataca atccactatg gagaaatata aaagaaaaga
    21181 gtataacaaa caataaaaaa taagatgatt caaattttat tttgaaacat cttatttact
    21241 ctacaaatta agattatttg gtgaattatt taattattat tttttattaa agaaatttta
    21301 aatcttaata taaaatacgc tattatacat aataatccaa atgcaaatgc tactggaatt
    21361 aacatctccg attcaaaata aaagaaatat tctaagaaaa tatctggatt tactatattc
    21421 ccattcttgt ctaaaaaatc ttttccatta tatcctaaat gctgtataaa attacttatg
    21481 ttattataat ctgcttccct ctcatatacc atattagcat tcaatccaga agaacttaaa
    21541 ttagtgtttc catctttcat atctattaca tatagtctaa gagctttgac tttactatac
    21601 ttatctttat caagtatttt ttcaagttct aacttatatt caccaatctt tgtcatatca
    21661 tgtctttctg ccaattgctt tatttctcca tcaagttctt ttcctaaatt atacatatta
    21721 tgttgtaaac tattattgta ataccacata aatccagaaa ttaaaaatgc aattatactt
    21781 atgtaaccta atactttttt aaaccccatt acaattgatt tatgcctatc caatgataaa
    21841 gatagttctt ttattatatt acataattca tactgcattt catcaccatc atcaaatctt
    21901 tcaattgctt ttttacatgc ttcttcttca tctaatcctt ctaatttaaa ttcatttgca
    21961 gattctataa gatggcatct catttcctgt tttaattcta tagtactttt attgtcacat
    22021 tttttatata gtttatctaa atattcatct attagtttca taaattaatc cacctctccc
    22081 aaaaattgat tcataacctt tttaataaat ctccattctt gtttctttat ttctaaaaaa
    22141 tctttacctt cattagttaa attataatat tttcttcttc cacctgaact ttgttcattg
    22201 ctataataag atttaattaa atttttagat tccattcttt taagtgcaag atacattgtt
    22261 ccttctttta attcaaactc acttctttct cttacttgct ttgcaatttc atatccataa
    22321 caatctttct tttctaatat agaaacaatt aatatatcga tataaccttt taacacttct
    22381 ttatttaact gcattatgta ccaccgcctt ttcacctact actctataat gtatagtact
    22441 tatatttact ataatattac atagtactct atattgcaag gtttatattc tacaatagac
    22501 ttaaaaataa taaatagctg tatattaata tttaatctat aaatactaat atacagctat
    22561 ttatcaaatt ttaaaaataa atttttgttt tattaatgtt gtcataacaa catttacttg
    22621 gttgaaatag tattacctat aagtttaaat atatataata atagacttga ttctatttca
    22681 tattattaaa atagagtaat agtttaaatt ctactactct atctataatc tatttcctaa
    22741 ttatttaatc tcaaatttag ctcttcatca tttaaatctt tcataagatt tctacaggta
    22801 ctagatttat tccaacagaa agactagggc attaaacact tatatatctg tttctttaga
    22861 ctgtaatatt aatatttttt aaatacgttg acttttgctt tttctaactt ctctccatga
    22921 tagttctcct ctatcaagag cttttataaa tatttctgct gaagctagat ttgtagctat
    22981 tggaacatga taaacatcac aaagtcttat taacgcttgt atatctgttt catgagcctg
    23041 agatgtcaat gggtctctta aaaaaataac taaatctatt tcttgtgtta ctattaaaga
    23101 acctatttgt tggtctccac ctaaaggacc agatgctaat ctatttatat ttaattcagt
    23161 ttcatccatt attctttttc ctgtagttcc agttgcatat agtccatact tttttaaaat
    23221 tgattcataa cctatacaaa atccgaccat agtgtttttc atttggtcat gtgctactaa
    23281 tgctatattc atatatctaa tctcctcaat ttatgtttag atgtatttaa atttagaaat
    23341 ttattttctt tcttctcata cacacattta gtactaatcc taagtttgct aaagatgtca
    23401 ataacgaact tcctccataa cttacaaaag gtaatgttac tccagttact ggtattaacg
    23461 caaccgtcat acctatattt tggattatct ggtaaccaaa cattcccata acaccaacca
    23521 ctatgagggt tccataaaaa tcttttgcat ctcttgcaat tgctaaaagc cttaatagga
    23581 atatcataaa taagattatc aacactgcca ttcctattac accaagttct tcacctacaa
    23641 cagcaaatat aaaatcacta tcttgtacag gtaaaaaatc ttcttgattt tgacttccat
    23701 tgtaaagtcc tttaccagtt acgcctccag aaccaattgc aatcagagat tgcataactt
    23761 gatagtttcc ttttagagtt acatcttctg gatttagaaa agcttctatt cttacctttt
    23821 gatggtctgc catcattaaa tacatcagtg gcattgacac taatagtatg attattcctc
    23881 tttttatcaa ctttgagcta agacctgctg tgaacagcat tgcaaatatc atacaacaaa
    23941 atacaattcc tgttcccaag tctggttgtg caatcaataa tccgataaat ggaagagaat
    24001 aaactacaac aggcataact tcctttaacg tatttaattt atcctttttg gattcaagta
    24061 tctttgcata acttagtaca aatgttaatt tgactatttc agatgtctgc aagtcaagtg
    24121 gtcctaagtt aatccacgac ctagctccac cttttactgt cccaattcca ggtaataaaa
    24181 ctatggctaa caaaataaga cttattatat aaagcgcttt ataatacctg cctagaaggt
    24241 tatagtcaaa aaataatatg actataatca ttcctatccc aagtacaaaa gctaatcctt
    24301 gtttaatcaa ttgattatat gaacctgttg aattagcatg tgttgcacta ctaagtataa
    24361 caagcccaaa aataaagata gctaatactg taactataag tttccagtct aactgtttta
    24421 tcaacttaat tgtcgactta taattaatat tgattttttt caaagaatca ccgtctttct
    24481 atctttaaac atataattaa ataaaagggg catataccaa taaggatatc ccccttttaa
    24541 gcactaatta catacatcta tcttttatat tctttagtgg tatattagca actaatgcag
    24601 atacaggttt gtcatcatgc tcacctctgc atttagacat ttttatttca agaccatcat
    24661 cttctatttc tgcataatta gctattactt ttaaaatgtc tgtttttatc atctctaaaa
    24721 gttgtggtga acaatctact ctatcatgaa ctaaaactag ctttaacctc tctttagcaa
    24781 cagatttact agttttagcc tcgttagaaa aaactctaaa taaatctaac acagatgtat
    24841 cctcctttgc tacttagcca tgctaaacat ttttttaagt ctaccaaaga atccatcttc
    24901 aacttcaaga tcaagtagag gaacttcttc atttagtatc ctttttgcga tatttttgta
    24961 tgcttgacca gcaagtgact tagaatcaag tatagctggc tctcctttat ttgttgatat
    25021 aattatgctt tcatcatcag gaacaagacc taacaaatct attgctaata tttctattat
    25081 atcttgttta tccatcatgt ctccacgctt aaccatatca tttctaattc tatttataac
    25141 taatcttatc tcttttattt catttgcttc taatagacct attattctat cagcatctct
    25201 tactgctgat atttctggat tagtaactac aatagctcta tctgccccag ctactgcatt
    25261 tttaaagcct tgctcaatac ctgctggaca atctattatt atatactcga aagattcttt
    25321 tagtttctca cacaggtcaa tcatctgctc aactgagaca gcatttttat ctctagtttg
    25381 tgctgctggt aataaatata gattatcaaa tcttttgtct tttattagag cttgcttaag
    25441 cctacaagtt ccttcaacta catctactat gtcataaact attcgatttt caagacccat
    25501 tactacatct aagtttctaa gtccaatatc tgcatccaca actactgttt ttttattttc
    25561 taggcttagc gcagttccta agtttgctgc ggtagtagtt tttccaaccc cacctttacc
    25621 agatgttata actataactt cgctcataat actaccccct atttttcttt ctatttgtct
    25681 aactttgaca aatagctttc gattacaatt cttccattac ttacaaaggc tatttctggg
    25741 ctaatttctg attcactttc ataattttct tcatcatctg gagcttctgc aatattatct
    25801 gctatttgta atattttagg attaagattt ccagcaacaa catatgcaaa ttcattgcct
    25861 tctgcacctg catgtacaaa accttctatt ttacccatta caaaaacatt acctcctgct
    25921 acaaccttag caccagcatt tatatctccc ataacaactg tatttgattt tgaaaggact
    25981 tttgaaccag gtttcatatc attcaatata actacatcac cttcaaattc aatattttca
    26041 ccagatctta gggtattgac atattttgtt ggaaaattac tattttcttc aattttttga
    26101 tcttcaacaa attccacatc aaatctagaa gtaattcctt ctttaagttc tagaatctcc
    26161 atgtcagtta aacaatcact attaatttta ctaattttag ccccattaaa aaagccaaca
    26221 tatgcttcca atttgtttat tattttttcc tgtatttcct caaatggagc ttctcttttt
    26281 atatttacta ttatccctct tttattaccc ttgaactcta ctagttcttg actacaaatt
    26341 tctcttaaag acataacaac ctccagagtg atatatcatt tatattcttc aaatacgctt
    26401 taaacccttt tattttttct atataaagct aaagtttata catagattga gctatctatt
    26461 aatttgtgac tcgaaattta tattttcgtc agaagaattg ttaacatcat tcgtcttgtt
    26521 gtcattttta ctattagttg gctctaatcc catataagtt ccaataactt ctctagttgg
    26581 taaaagtgag aatacacttg aatcaccttg aggaattaca catacaacag ctatttctgg
    26641 gtcatctgct ggagcaaaag caacagtcca cgtaaaagaa ccataatctt gtttaaatct
    26701 atctatttgg tcatctgtta tttttggatt taattctttt atagcttttc ttagatatga
    26761 agaattcacc ttatcagtgt cctctaatct aacttttact ccatcttcta attcttcctc
    26821 taatttcttt ctttcttcgt ctttcaaatc tttattttct aatttctttt ttatttcctt
    26881 ttccttagct aatgaaagct ctttttcttt ttctgctttc atcttatcag ctagtttaat
    26941 cgcatcattt aggttgacat tataagatga catatgactt tttaaatatt catactcgtt
    27001 atctgtaggt attttcccac ttttctcagc tgtaccagtt tttgctgcta catctattgg
    27061 aaaatcagca aaagcactct tcgctgtacc ttgtcttgca accaatttca taccttttgt
    27121 taattctttt aagttatctg gatttttaaa aggtattttt tcgactttct tctggtcatt
    27181 aattttaact gatgaataat cacttgaaac agctctatca actacagata attctactaa
    27241 attacctcca tttgctatag cagctacata tcttgatatt tgagctggtg tataagcatt
    27301 ttcaccttgt cctatagcta agttgaatgt atctgcagta ctccattttg caaaattaaa
    27361 ataactaaat actgctaaat ctgctacttc ttcaattcta tcttctttaa catccaattt
    27421 ttttagccta ttcattgttt caactctacc aggagttttc ttttctgctg cccagcttac
    27481 aatctcattg attcttttct catattcttt aggatttttt tctctagtta tatcaacaaa
    27541 atcatttgcc atttctcttt ctaaatttga ctttagaaga gcttgtgttg atttcaattt
    27601 atcttctgtg ctaggaactt tacctgctct ttcttctact tcatcttgaa gccctgtatt
    27661 ttggtcaagc ccaaataatt ttgcatactc aagtatttta tttgcattca tatctatacc
    27721 aatgcttttt ccatctggcc atgttttacc tgtaccaatt gttgccatat atatattaca
    27781 agactcttgt attgctttat ataagtttgt cataccatgg tttcctctac catggttcca
    27841 tacatagtca ccaaaagatt ttttacctaa cattataacc ccagggtcat ttatagtaaa
    27901 gtttggatcc agaccatttt ctaacgctgc cattgaagtt accatcttaa atgaagaacc
    27961 tggctgaaat tccccttgtg taaccaagtt taaaagtgga cttcctgcta gtaagtcatt
    28021 tggatttttt ggttgaagtg ctttataatc ctctgtagaa ataccttttg caaatttatt
    28081 tgggtcataa ttaggataac ttgatgaagc taatacttct ccatttttaa catcaatagc
    28141 tataagagct gctgactgtg cttttcctgc atatgcacta attggtttgt ccccaaattt
    28201 acttttaaaa gtacctcctt tagaagcaac ttcgattatc tgttttaatg cattatctga
    28261 aacttcttgt aaatctttat ctagtgtaag atacacagta tctcctgatt taggtttttt
    28321 agattcaatt tctttactga ttctacccaa tgcatcaact ttaaccattt tataaccatc
    28381 agttcctctt aatctacttt cattactctt ttctactcct gcaagaccta ccatatctcc
    28441 agtttcataa cctttttgaa ggtatgattc tatttgagtg gatggcattt ttcctacata
    28501 tcctaacata tgagaagcta atgacccatt aggataatat cttactggtt ctactgctat
    28561 tgaaacacca acaaggtcca tagcactttc ttcaatttga gcaatcgtag tttctcccac
    28621 atccttagca atagttactg gattatattg agaatatcct tttgatttta tcaaatccct
    28681 tacgaccatt atttttcttg catcttcatc actcaaactt ttatcaattt ctaatgcatc
    28741 actatttctt actttttcaa acgcttcttt ggcactcagt ttagtttctt ttatcttata
    28801 actagccaac caatctctct tatctctttc tgctgtgaac atccatttac taactaaaat
    28861 aggtggataa tatccatttt catttagttt tgcctgtaat cttgtcgcat ccaatctttt
    28921 atcttcttga gataatattc cagctgatat aagtttgtcc accaagtaat aaaaactttc
    28981 cttagcatta taatcatttg gaataccatt ttctgattta tattccctaa tatctctgtc
    29041 atatgtataa tagtactttc cattttctac atatatagga aactcatcta catattcttc
    29101 accatttctc tccagcaaat ttataagttt taatgatatt tcatttgctc ttgaatgctt
    29161 atttgcatct ttcttattaa tatcgttacc tgaaacttga acagtaaata agtttttatt
    29221 tcctgccaac agtcttccat atctatcttt tatttctcct cttggagctt ctattgccaa
    29281 ctttttatat gttttattct cagctaactc attgtaatat tcatatttaa atgtagtcat
    29341 atagattatt tttactaaaa tagctaaaaa tatcacgccc atgactatct taattatttt
    29401 tagtctatca acttgtttat tttcttccat gaaatcacca actaatcttc tttaagtttt
    29461 aaaactgctc ttttagataa gcgatatatt actaaaccaa ttaaactatt cactattggt
    29521 gctactagta gaccttttac aaaaagtgtt gatatgttgt agctttgata aattatacta
    29581 gatgtaccta tattgacaat tgaatcaatt aaactgccta taaaaattaa aacgaaagta
    29641 ataatataac tacctttata tagctgatct ctcatgtatc caattgcata tgcaataata
    29701 aacaaaatta atgcatttac accaaaaatt ccaccaacgg ttatgtcttt aataagccct
    29761 attattgctg ctataatccc accttctaat tcatctaaat aaagagagat tatggttaca
    29821 tatataagaa ctaaattaaa gcttatgcca aaaatatcta tataatttgt tatactattt
    29881 tctatagtca taagcacaat ccccaacaga caaagtaaaa cttttttcat tgtttttatc
    29941 ccctttatcc tatatttctt ggttctatga ctacaacgtc atttatattc ttaaaatcaa
    30001 cataaggttt tacaactaca tattttaatg acttatcttt atcatcaacc actttttcaa
    30061 cttcgccaat tggtatacca tctgggaaga atccaagacc tgatgttgtt attatatctc
    30121 cttgaattac attatatgat gagtcaaaca tatatccttg caaatatcct ttactattat
    30181 aactatcttt attatctaaa gtagtatttt gagtaattgt acctttagca ttggcatcct
    30241 ttgctagttt aaagcttaca gatgccttag aatccaatag tgaaattgcc ttactataat
    30301 tgttggaaac ctcataaact atgccaacta atccactacc attcataact atactttctt
    30361 tttttacacc tgaatttttt cctgcaccta tgacaaaact ctcataccag tttccatcat
    30421 ttttaccaac aacacttgct gaaatacttg tcgccttata ctcttcttgt acaaagttca
    30481 gtgtcttttt taactcttct agcgattctg ttttatctag tttggcattt aaagctatca
    30541 tatttttttt aagtttttca ttttcttctt ctaatttttt taccttttta gaattatctt
    30601 taaaatttgc tacatcttta aaaccatttt tgataaaaga aaatccatca tttattccct
    30661 tacctacact tgtaatagca tctgaagcta ctcctaaatt tactggacta ccactagaaa
    30721 atttaccaat tgatataccc acaattccaa ttaaagtgat agcaactacg cccgttgcta
    30781 tcactttaac attaatcctt tttttctcat ttttgttctt gtcaaatctc aaggccatca
    30841 ccaacttcta attcttagta ttcatcatta atactttttc aaagatttct tggtcttcaa
    30901 cagatttacc tgttccaagt gcaacacaat caagaggtgt ttctgctatt tgtacagaca
    30961 taccagtttc ctgtgtaata agcttatcaa gacctctaag caaagcgcct cctcctgtta
    31021 gcattattcc gttttccatt atatctgagg ctaattctgg tggagttctt tctagtgtag
    31081 attttattgc atctactatt gaacttacag gttcttttaa tgcttctcta acttctgtag
    31141 agcatatttc tattgtttta ggtaatcctg agattaaatc tctacctctt atttgcatat
    31201 ttatttcttc atcatcttta aatgtagaac ctatgttaat ttttacattt tcagcagttc
    31261 tttcacctat cataagatta tattcttttt ttacataagc cactatagac tcgtcaaatt
    31321 cgtctccacc tattcttata gatttagcag taacgattcc tcctaaagat attacagcta
    31381 tttctgaagt acctccacca atatcaacta ccatattacc ttctggttct tcaactttta
    31441 atccagcccc tatagccgct gccattggct cttctataag gaatgcatct ttagctcctg
    31501 cttgtcttgc agcctcttct atagctctct tttctacttc tgtaactcca aaaggaacac
    31561 atatagctat tcttggactt acaacacctt ttttatcagc cgctttttgt ataaaatagc
    31621 taatcattga ttgagttatg tcaaaatcag ctattactcc atctttcata ggtcttatag
    31681 ctactatatt tcctggtgtt ctccctatca tttttttggc ttcttcacct actgctaata
    31741 cctctttact atcatctctt attgcgacaa ctgatggttc tctaacaaca ataccttgtc
    31801 cttttatgta aactaatgta tttgcagtcc ctaaatctat tcccatatcc tttgtcatct
    31861 tattaaaact aaaaaaacct tttttttctt tctttttttc cttagccatg attttactcc
    31921 ttcctactat taagaatcat gtttaaaaca ttctcctatt attttatcac atataaagtc
    31981 aaattatcat attttcttta aagctaaagt ataatccatc tccaataatt atatggtcta
    32041 ttatttctat tccaattatt tctccacatt taataagtct tgatgtgata tttttatctt
    32101 ctcttgatgg ttctacactt cctgaagggt gattatgcat cactattatc ttacttgcac
    32161 ttcttcttat agcttcttta aaaacctctc ttggatggac taatgacgag cttaaagtcc
    32221 ctatagatac atctacatca gatattatct cattttttgt gtttaaaaga accgctttaa
    32281 aaacctcttt ttttaaatac cttaggctat ccatataata cctctgtata tcccaaggat
    32341 tccttatttt atactttata ggtttaaaac tagctactct agagcctagt tctaaagctg
    32401 ctttaatttg ggttgatttt gataacccaa ttccatctat attacacagc tcctctattg
    32461 tcatatcttc taaatgtcta ataccctgaa tatctctatt tattatatag ttggctaatt
    32521 caatagcatt tttattcttg ttgccagttc ttaaaagtat agccaacaac tcagcatttg
    32581 ataagctttt tacgcctttg gcaagcatct tttctctggg tctttcctct ggtgccattt
    32641 cttttacctt aatggttgaa ttaaatgact ttttcagata aatcacactc cataaaaaag
    32701 atttatacta aaatattttt ttaataggtc gcctaacctt gaaattggaa gaccaactat
    32761 attaaaataa tctcctctaa tttcttcaac taacaaagct ccataaccct gtataccata
    32821 ggctcctgct ttgtcaaatg actcattagt tttcagataa tcctcaatat cctgctctga
    32881 taacgtttta aatttcacat tgctgattac atagtcaatt acctttttat cttcgcacaa
    32941 gtttattaag ctaatacctg ttatgacttg atgctctctt ccagatagtc tttttaacat
    33001 atctcttgca caagactcat cttttggttt tcctaaaata gcattgtcta aaacaacaac
    33061 tgtatcagct ccaataacaa catcttcatt atgttcagat gctacagaca tacttttttc
    33121 aaaagctaat ctcataacta aatgttttgg tgtttcacct tccaatataa tctcatcaat
    33181 ttcattttta attatatcaa atcttacatt cgtattctct agaatttctt tccttcttgg
    33241 agaggctgat gccaagatta tattcattta ttcacctcat ttgtacgtaa tttgtaccta
    33301 tttagtctat cactatattg taaaaaattc aacagaaatg tcgaaccttt tttgttacac
    33361 tattgttata tttattacca ttagaataaa tatacataaa atagaggtat ggaaattatt
    33421 ccatacctct gttttatgta ctcattagag atatggaaat tattccatat ccttattttg
    33481 tccatattac aaaattttaa tagctttctt tggacatttt tccatacata aatggcatcc
    33541 tacacattta tctgcatcta ccttatgttt ttcttttaat tctccctcta ttgcatcaaa
    33601 cttacattgt tttttgcaca ctgtacatcc aacacaaagt tcttggtcaa tagtaacttt
    33661 ctttttctta gttatgtcac ctgatattac ttttgtaggg cattttgcta cacattgcat
    33721 acaacctaca cacttattag ggtctatctt agctattttg tcttcaaata ttattgcatc
    33781 aaatttacaa gcttttacac acattccaca gccaatacat ccagcagtac atttttcttt
    33841 aacagcttta ccaaactctt tgctattaca ctctacaact acttcttgac tttctggttt
    33901 tgtgattatt atacccttag gacaaacttc tttacatttt ccacaattta cacacttttc
    33961 ttcatctatt actgctattc catcaactat agaaatagca tcaaatttac aagcatcttt
    34021 acatgttcca agacctaaac atccaaattt acacatctta gcacctgagt ttaaaacatt
    34081 tgctgctcta caatctgata ttccttcata ctcatattta tcttttgcac ttgaacaagt
    34141 tcctttacaa ataacttttg ctacttgttt ctcacctgct tcagcactta ttcccattat
    34201 ttctccaact tttgcaccaa catctgctcc acctacaggg caaccattaa ctggtgcatt
    34261 gccttcaact atcgcatttg caagacctcc acaacctgga aatccacaac ctccacagtt
    34321 ggctcctgga agtactccta gtatagcttc cactctctcg tctacttcta cagcaaactt
    34381 tttagatgca aaatctagta ctatcccgaa gattaagccc ataattccta aaactaatac
    34441 agctgtaagt atcaccattt tcacccctct tttttatagc tttataagtc ctgtaaatcc
    34501 taagaaagct attgacatta aacctgctga tataagcgtt atagggaaac ctttaaatgc
    34561 ttctggtata tcagctaatt ctagtctttc tcttatacct gcgaatatta ctattgctaa
    34621 agtaaatcca gctccggctc caaatccgtt tattattgtt tctactaaat tataactttt
    34681 ttgaacatta actagagcta taccaagaac tgcacagttt gtagttataa gaggtaaata
    34741 aactcctaat gcttgatata aagatggact catcttttga attaccattt caacgaactg
    34801 aactatagaa gctataacca atataaaagc tattgtttgt aagaatccag ttccagtttt
    34861 tactaataaa atctgtataa aataagttat aattgaagct agagttaaaa cgaacgtaac
    34921 tgcaactccc atccctactg ctgtatcaac cttcttagaa actcccaaga aaggacatat
    34981 acccaagaat tgagatgtta taacgttatt aacaagaacg atacttaaaa ataaaagtat
    35041 taaattcatt tattacacct ccactatgct tttttacttc ttaatttatt aaatcctgca
    35101 aataagaatc ctaatgttaa gaatgcacct ggtggtaata taaatattaa tactggttgg
    35161 aaagatgctc caaataatgt cattccaaat aaactaccat ttccaagtaa ttctcttact
    35221 gcacctatta ttgtaagtgc aacagtaaat ccaagacctt gacctacacc atcaactatt
    35281 gaaggcatag ctcctgtttt gaaagcaaaa ctttctgcac gagctaatat taaacagtta
    35341 acaactatta atggtatata tataccaaga gccttatcaa gagcaggaac atatgctttt
    35401 aaaagcatac ctactattgt aacaaatgta gctataacta ctataaatat aggtattctt
    35461 atcttatctg gtgtaatttt tcttattaaa gatataacta aatttgcaca tattaaaact
    35521 gctgctgttg aaagacccat accaattcca tttattgctg aagttgtaac ggctagtgtt
    35581 ggacacatac ctataacttg aacaaatgtt gggttctcat ctattagccc atttttaaat
    35641 actttagcta aattcataat taaccctcct tatttattaa gagcactatt gaaaacatct
    35701 atagcagcat ttactcctgt agtaactgct gtagatgtaa tagttgcacc tgatattgct
    35761 aatatttgat tatctgctgt tgtacttcct tttgctactt caagaggctc agcagttttt
    35821 cctttgtatt gacccttaaa tgcatcatcc ttagcttttg ctccaagacc tgctgtttct
    35881 gacatattac caatgtcaac acctgtaact tgaccttcag atgatatacc taccataact
    35941 tcaatttctc caccatatcc acttggttta gcttttaaag tatatccaac cacttcagaa
    36001 ccatctaaac cttcatacac ttcagctata agaccatctc caaggccttt gtaagtatct
    36061 tccttcattt ctttaaattc ttttgcttct ggaaggactg cttttctaag ttcattattt
    36121 gcttgtatat ttctttgttc tattactgga gctgtaacct gattagtagc tccaagagct
    36181 aatgcagcta ttgcacttat agcaagtaaa gtaccaccta atctcaccat actgttcatt
    36241 atttagccac ctccccaaat actctatttc taacaaactt atctataagt ggagcaagac
    36301 agttcactaa tagtattgag taagatacac cttctggata tccaccatac attcttataa
    36361 cagttgttat aagaccagct aatacagcgt atattatttg acctttttta gtcataggcg
    36421 aagttgtata atccgtaagc atgaagaatc cacctaacat aagtcctcca gcaaacagtt
    36481 gatatattgc gaaagttata ttaaatccac ctaaaaggaa tgttaatata aatacagttg
    36541 ctatgtaaaa tactggtata acataattta ttatgccttt atacattagg tatactccac
    36601 ctaataataa taatattgct gatgtctcac ctatacatcc accaatattc cctatcatca
    36661 tgtcagcaag actaattcca ttgctagaaa tatctgccag tcctgctgct ccatttttca
    36721 aaaagcttaa tggagttgct gtagtaactg catctaaatt tttacctatc cagttaacac
    36781 ctggttgagt ccaagcagtc attgctactg ggaatgatgc taataaaaat gctcttgcag
    36841 caagtgctgg attgacaaag ttacatccaa ttccaccaaa taccatttta actactaata
    36901 ttgcaaatat tcctcctact agacacatcc accaaggaag tgatgctggt acgttaaatg
    36961 ctaataataa tcctgttaca acagctgaaa aatctcctat agtacttttt tgcttcgtaa
    37021 atttttgata taaaaattca gtacctactg taccaagtat acaaaaaaac atagcactca
    37081 aagcattaag tctaaagaag tatactcctg ctactgctgc tggaaggagt gctataataa
    37141 cttgtttcat tatatatgaa gtatcttcat tacttctcac atgaggagaa gatgatacta
    37201 tcaacttatt ttccatcgtt tttccccctt aactttcttt atttctgagc agcttgcttt
    37261 cttctctttg ctcctatttc tctctttgct gatcttatag attgaagtaa tggtcttctt
    37321 gctggacaaa taaatgagca agacccacat tctatacaat ccattgctct atgatattca
    37381 gctgtgtcat agtcatcttt taaagaatat gcacttatga aaagtggttg taagaatgat
    37441 ggacatacat ctgtacatct tccacatctt aaacagtttt gaatatctgg agtacgtgat
    37501 tcttcctcat ccaaacataa aattcctgaa gaacctttgt tagtcgctat ttcagtagta
    37561 tattgagcta ttcccatcat aggtccaccc attataacct taccaatctt cttatcttct
    37621 ttaaatccac cacattgttc tattatttct gaaactaatg ttccaacttt tgttattagg
    37681 ttctttggct ctttgatgca actacctgtt attgtagtga ttctttcaac caaaggcata
    37741 cctgttttaa tagacttagc tatctgtgct gctgtggcaa cgttgtcaac aactgcacct
    37801 gctgctattg gtaatccacc agatggaact tctttgcctg tacacgcata aataagttgt
    37861 ttctctgctc cttgtggata tttcacttga aggccaacaa cttcgatttc gctatagtct
    37921 ttagctacat tttgaattgc ttctatagca tctggtttat tcgtttcaat tcctataaat
    37981 ccttttttga catctaatac cttcattaat gctcttaaac caaacacaac atcttctgga
    38041 ttttcaacca ttaatctgtg gtctgcagtt aagtatggtt cacattctgc accatttaat
    38101 ataactactt ctgccttgct atctggtgga ggagatactt ttacatggtt agggaatgta
    38161 gctccaccca tacctactat tcctgcctct tttatgatac ctactatatc ttctttgctc
    38221 aaatcctcta actgaccttt tggttgaaca ctctcatgta attcttcctt aaagtcattt
    38281 tcgataacta cacattgagc tgaacctcct ggtacttcat gttgttcaat agcaataact
    38341 ttacctgata ctgaagaatg aacgttacat gaaacaaaac cttgttgttc acctattttt
    38401 tgcccaaact ttacttcatc accaacttca acaataggtt tagctggtgc ccctatatgt
    38461 tgctgaagag gaatgtaaac tatctttgga gcttgagctt tttcaagtgc ctttgtgtta
    38521 gagtactctt ttctatatgg aggatgtatc cctcccttaa aagttaagag tttcattgtt
    38581 ttaccccctt atctataaaa aaacattttt tctgccttct ataagtatac tatatatatt
    38641 aaaaaatctt aacaattgtt aaaaaaatat ccgtattttt ataatttatt ttataaatca
    38701 acacctctat attaagtcaa attgaggaaa aggattgctt aattaaacaa tccttttcat
    38761 atagctttct aaagtccaaa ttttctgtat tttattttat agaatttata aacatagagt
    38821 actcctgcat ggatgataaa agcagacttt tacttacact atatttttct tcatttgctg
    38881 cttttgaagc tgtatcaatt ttttcattca cattctttac atactctatt aaattatctt
    38941 taaataaacg catttcagaa taatcaatct tttcagcttc ttttgatata ttttgtgata
    39001 ttgcctttct atttgtcaaa atagtattat atttttccat atctacatta ttttcagcat
    39061 tcgaccaaaa acttgattct tctttaaagt ttgttattaa tttatttaat tctttagata
    39121 tactctctat ataagcgtag ttacttgtat actctaaaga caacataggt gcatctaagt
    39181 gtgataaaaa tgcatctgga aacacctttc ttacactgct tatctgcttt ctaacatcat
    39241 tttcatcaaa agaggaatag gtctgtattt tttttactcc atccttctcc ataactgaaa
    39301 aaggtatgtc attattaact aatgatgtct gtattttttt caaatcatca ttattatcta
    39361 ttgcagcaac ttgaatagta tacatatccc agccttctat agtagctaat ttgacttctt
    39421 ctggctcttt agtgttattt accgcttttt ctggattagt ttcttgttta tcatctgaat
    39481 tggtttgtga tttagatttt ttcccttttt ttattgaatt taattcatca gatatatctt
    39541 tataagttat atctgatgaa ttatttagaa atggtatttt agccgataca tactccaata
    39601 tcttgctatc ttttattttt gtataagaat aatagccaat tagacacaag caagcaatcg
    39661 ttataacata tttacgtaaa tcaatcttat gtctattatt attaaatcct ctgtatatcg
    39721 tttttcttct attcattagt ttgtccctcc caaaattata ttattaataa agtatatttt
    39781 taagagtcac aatttatgaa tataaataat tatttcttct aattttttaa taagaaattg
    39841 tacttactaa acaaatcttc ttgcaccaag taatacttta gaccaatatg aactagttaa
    39901 attatccact ttaaccttat taggattagt ctgagtacca ctacaatgga taaatttact
    39961 atttcctacg tatataccta catgattagt tgttccatca ccatcagtgt caaaataaac
    40021 taaatctcct ggtgcataat ttcccataga aattgcactt ccaaaattag cctgttctct
    40081 agaaactcta ggaatagaaa cgcctacaga atgtttaaaa acatactgtg taaatccaga
    40141 acagtcaaat ttatcaggtc ctgaagctcc ccattgatat ggtattccaa taagagtata
    40201 tgcaaaatct acgattgttg aaccttttgc tgtagattta tctgaatttc cagtaaatga
    40261 tggctttgag ttactattag attgagaatt ttggttagat gatgaatttg gtttttcttt
    40321 tgtatcctca ttagtctcag aaatataact ggcactcacc catccaattg ttccactcga
    40381 taactttacc ttataccaac cattactttt agcttttaat tcaacaacat ctccaccatt
    40441 taattttcct attatagaat agcttgtccc aggaccactt cttacattta aactcacagc
    40501 agatgttacc ttaccattct tgccttcaat tgattcttcg ctagatggtt ttttatcaga
    40561 attattagta ctatttgaat tactagaatt gttcgttcca acatcttctg atgttttaga
    40621 aatatattgg ctactagccc atccagtaac tccatttgat agttttattt tataccatcc
    40681 attgctttgt tctagtaact ttactacatc tccattattt gctttaccaa ctaatgaata
    40741 gttagtacct gcaccacttc ttacattcag tctagaactc acatttactt taccatttcc
    40801 actaatagtt gtcccacttg aagttggatt attttgactt gatgtacctt cagacgaacc
    40861 acttgttgat atgtattttg cacttcccca tccaacaaca gaactcgata cccttacttt
    40921 ataccatcca ttagactttt ctaaaatctc tacagttttt cccttgtata attttgctac
    40981 cttatcgcaa tcagaacttg gtccacttct tatattcaag gcacttgctg ttactgttcc
    41041 cttttcaaga gcacttgcat ttatggagct aacagatact gctactgccc caattcctaa
    41101 agctgctata gcttttttca ctactatcac tccccccaac aaatttttat agtcataaat
    41161 tcataacatt attatccatt tttagtccct acaatttaat gataacttat tttcttatta
    41221 attgtaaaga aattataaac atatccttaa aattagtgat ttttaggaag tatttcaatc
    41281 caataattat ctgggtccgc tataaaataa attcccatac tttcattttc aaaacatatg
    41341 caatctaaat ctttatgaag tttttttgcc tcatcaaact cctcaacttc taaagccaga
    41401 tgaaattcat tgtctcctaa attatatggt ctatcccaat ctctaagcca agttaattct
    41461 aatgtatgac tagtaatgcc atctcctaaa taaactaata taaaacttcc atcttctgct
    41521 tccttgcgct ttacctcttt aagccctaaa gcttttttat aaaaatctaa acttttttct
    41581 aaattagtaa cattgaaatt gttatgacaa aaattaaact tcattttgaa atcactctcc
    41641 aatttttatt ttttttatat tatatcatta ttataaattt atttaaatca tattaagtta
    41701 aaaatttgaa tgatgatgac caattaccaa atcatggtaa attatttaaa agaatatgtc
    41761 aaaaaaagaa taagctcttt caaatgcata aaaaatttat acccaagtta tacttaatca
    41821 tgcaaattgg cagaacttat tctttaaaac tctaactttt aagtttttct ttaataatat
    41881 aatttattac tttttttcta atatcttcag cattataatc atcttctact tctatatcca
    41941 aaagttttgc tattgacctc atatcttctc tagtctttga cctatagctt gatattaatt
    42001 catcttttat tttctctggt tctaaaagat attcttcatc tcctcttttg aaaacaacgt
    42061 atttttgagc atatgaactt ctattgtagt atatatatct tgatattttt cttaaaattt
    42121 gattatatga aagtcgcttg ttaatatcaa gttttaagta ctctgaaaaa gcaaatagct
    42181 ctttttttga tttaaataat gcttctttct ttatagttct tgatattgaa gttacacttt
    42241 cttgatatgc acactttttt aacattttta aatatactct attaagacca atactttgat
    42301 tgaatttttt tatattatta ttaataatct tatccaagtt accttcaaag tatttttttg
    42361 ctcctttttg tgatacataa ctctcaagtt ctttgtgggc catatcaagt atcatttttt
    42421 ttaaatcctc tttatatccc ctagattcca tgatactccc tcccttagct atttatagta
    42481 tattcaaaaa gctcaagaaa tgtgagtcat tacaaaattt ttttactttt tatgtatata
    42541 ttaatttaaa aggggagata atagtaatgt aaaatcaaac atactcaatc tcccccattt
    42601 ttagacctct attactcccc caatagaggt ctttttttag tttatcaatt tctattcaat
    42661 tccctttagt tcaaatccta attctgctat cacttctttt aatttttcaa tagatacatc
    42721 atctttcata tctacagatg cacacttatt ttctaaatct acatctaaaa cttcaatacc
    42781 atctatatct tctgttaatg cagttttaag atggtttaca caatgaccac aactcatacc
    42841 ttctactaat agtttcttct tcatttaatt acctccttat accctataag ggtattctat
    42901 catatagttt atacttttta aatagaaatt aatcatttaa tttgattcat tcgattttat
    42961 atctaaatta gatattccct catatacatc aaaatcattt ggagtaattt taccttttaa
    43021 tctactatta cttacaatat agttgtttct cacagctaaa cttataaatg ttgcattatc
    43081 ttttatatat gtctgtacat ctttgtatgc tttttttgtt tcaatttcag acgtagaact
    43141 tgtaagtgca gacatatatc catttaactt atcatcagta tatccactac tcgctataat
    43201 actacttgca tctggtataa ttgatagttt ccatccaact aatgctaaat cataattttt
    43261 agaattaaga gcattttcca tatccttatc tgacagctga tttactgtac tatttattcc
    43321 tatagctttt aagttttcat ttattatgtt tgcagttttt attctctcac tattatctct
    43381 gtttactatt atctttaagt tcatttcaga aagacttttc tttacttcct ctttttctct
    43441 attttttttc tcttcagctt cttttttgat tctatcttct tctgcttttt tagcttttgc
    43501 ttcttcttct ggcgtaagct tctttgagtt ctttttagct gattttttct gattttttac
    43561 tttattgtca ttttgattta ctgagtttac atttgcatat tctaccttag caagacactc
    43621 tatcgcctta tctttattaa actctaaatc cttcatttca ctattatagt attttgattt
    43681 tgaattaagt ggaaaattaa ctggagtagc atcatccata taaccttcac taataattct
    43741 atctttatct atagagtgtg caattgcttt tctaaaatta acatctttga aaaaaggatt
    43801 attataatta aataatacac actcataatc tcttccttga tattttgtta tattaaactc
    43861 tttttcttga aacttactta aatcatttaa tgtaacattg gttatatcac tatcaagagc
    43921 cataaccata gatgtccttg catcttcatt aggcaccata cctactttta tgttcttcat
    43981 ggtttttgga acttcttcat agtaatcttt gtttacagat aaaaccattc cttctctttc
    44041 tgtatatgat tctattttat atttcccatt tcctatcata ttattattct tgtcatttac
    44101 atcttttgaa tctaaatgat tttgagatac aattggaaaa attaaactat caattgaaaa
    44161 agcatatcta gctttaaatt ttattgtaaa atctttatct gatttaatat tcacagaagc
    44221 tatattttta gtaaattcat tatatggact atctgcactt ttttgaatta aaccaatagt
    44281 atagtttaca tcatttgaag taatattttt tccatcatgc cactttgcat cttttagctt
    44341 tatatctata ctcattccat cctgagctat attatactcc ttaactaatt gaggaattac
    44401 attgtagttc tcatctatag taaataaact atcataaact aaattcatta tataaccaac
    44461 agacttatca gtattagtta taggatttat agtttttgga gttaccattg ttaagtttat
    44521 atacttagca tctatagaac tttgactttg taaacttgaa tcactactac tttgttttgt
    44581 tttagtatta ctacaaccag ctaccataag agttatagct aaaacgactg ttaagacttt
    44641 tattctcttc attagttttt tcctttcttg ccatatatct tattataatg ttcgtaatta
    44701 ttttttactt tttctacata ttttgaagtt tgcttaaaag gtatatcatg tagattttcg
    44761 ccatctttac tatattcatt attttctaac cattttttca cattacctga accaccatta
    44821 tatgcagcta tcactaaatc taatttacca aattctttgt ataacttact taaataccaa
    44881 cagccaagtc ttatattagt ctctgggtcg aaaatatcca cattttttaa atttagttcc
    44941 tctgctcccc aatctctagt tatgtctaat atttgcatta atccttttgc ttcctttttt
    45001 gaaacagcag aactattaaa cttactttcg gctttaataa cactgtaaac tatattttca
    45061 tctaaattaa attctttcga atacttttct acatactctg aatatttttt aggatataaa
    45121 aatttatgta ttactttgct ttccattaat agtgccccaa ataagattat aaaaatagat
    45181 agaatcaata ctttcttact attcacgcca attctccttc atgtactcaa taaatttatg
    45241 tgctttactt tctaattcag ttattgttcc cgaattatct attatataat caccatattt
    45301 ggatttttct tcctgactca tctgtgaatt tatcctacta agtgcttcct gctcactgca
    45361 attatctctc ttttgtattc tactaatttg tacttcttgt ttacaagtaa ctactaatag
    45421 cttgtcaacc atatctaaaa aaccactttc taccaatatt gccgcatcta acactactat
    45481 attttcgcct ttttttctca atttttctat ttcacttatt attttttctc ttattcgtgg
    45541 atgagtcaag ttatttaatt ccttaagttt ttcttcatca ctaaaaacta ttttcccaag
    45601 agcttttcta tttagagttc catcatcatt ttttatactt tgcccaaaat ggacaaatac
    45661 tttttccaat aacaacttat cctcaaatat cttcctggat ataatatcag catccactat
    45721 aggaatgtta agatttctaa atatatttga taggctgctt tttccacagc ctattcctcc
    45781 tgtaagtcct aatatcaaca taaaatcacc tactttgtct cataccaaga actacctaca
    45841 tttaaatcta catctaagtg aacatccata tttacagcat tttccatttc ttcttttact
    45901 attttcttaa ctatatctat ttcatcatca attgcttcaa ctatcaactc gtcgtgaact
    45961 tgtagtataa gttttgattt caaattgttc tcttccaatt ttttatgaac atttatcata
    46021 gcaattttta ttatatctgc tgcacttcct tgtattggtg cattcattgc aaatctcttt
    46081 cctctatttc tttccataaa attgcttgac tttatttctg gtatatatct tcttctgttt
    46141 aaaattgtta cagcatatcc atcctcacta gccttttcta ctgttgtgtc cataaactct
    46201 tttattttat ggtatttagc aaaataactt tcgatatatt ctttcgcctt tggaacaggt
    46261 atatttaaat catcagctag accaaagtca cttttaccat atataatacc aaagtttaca
    46321 gctttagctg cacctctcaa ttcactagta acatcttcca taggaacatt aaaaacttgt
    46381 gaagctgttt tggtatgaat atcttcatga tgtttaaatg cttctatcat attttcatcc
    46441 tgactcatat gtgcaagaac tcttagctct acttgtgagt aatcggcatc aaccaactta
    46501 cagttatcat ctgcaataaa cacttttctt atttttctac ccatttccat ttttataggt
    46561 atattttgaa gatttggttc tgtagatgaa attcttcctg tagtcgttat agtttgatta
    46621 aatgatgaat gtattctatt actttctgga tttattatat ttaaaagtcc ttcaacgtaa
    46681 gtagaattta actttactat ttgtctatac tcagttatct tatctattat tggatgctta
    46741 tctcttaatt tttctaaaac atctgcatta gttgaatatc cagttttagt ttttttaatt
    46801 attggaagct ctaatttttc aaataatata acacccaatt gttttggtga atttatattg
    46861 aatggctctc ctgataattc ataaatttcc ttttctaatg tagctattat ttctttaaat
    46921 tcaacactca attcatccaa tatacttttg tctactttta taccttcata ctccatatga
    46981 cctaagactt ctactaaagg catctctaca tgataaaaaa gaccatccat actcatatcc
    47041 attagacttc tttccatcat aggaactgtt tctttaactg tgcatattat ttgacccata
    47101 tactctgcta aatcctcaaa ttctaaatcc tcatagtttt tagatttaac accctttcct
    47161 aataattctt cttttgattt aacctttttt gataaatact tcatagctat tgcactacaa
    47221 tcatatgagg tagatgaagt tgaatctatt aagtactcag ctattgttat atcaaaatac
    47281 atattttcta agtttatatt atatggtttt aaagatatat aatcatcttt taaattatat
    47341 ccatactttt taatttcatt atttgaaaat atactatcta actttgacac ttcatttgaa
    47401 tctacataat atattttttc tccatctaca cttataaaaa tatatattat atttttttca
    47461 agtatattac cttgtcctct aacagttttt aaaataactt gtcctttttt gtttacttca
    47521 tctataaatt tttctatatc ttttaatttt aatatttcta ttttttcttc tacgtgttct
    47581 gtgtcatctg aatcgaccaa atcaactaat cttgataaga gacttgtaaa tccaaagtat
    47641 ctaaatttat ctaaaagttc atctttatca taatcgccaa aaatcatact atctaaatcc
    47701 acttctattg gcacatctct tataattgtt gcaagtcttt tactttgaat agctatttct
    47761 ttgttttcct ctattttctt ttttacacta cctttaagct tatctgtatt ctctattaga
    47821 ttctctatac tagaaaactc ttttataagc tttatacctg ttttttcacc tattccaggt
    47881 actccaggta tattatcaga cttgtctccc ataagaccct ttaaatcgat aaattgtgtt
    47941 ggagtcatct catatttttc aattacagaa ttaaagttgt attcttctac ttctccaacc
    48001 ccttttttcg ttataagggt agttgtatta tctgaagcaa gttgaatagc atctttatct
    48061 ccagttacta tgtatacctt atatccattt tcttctgctt ttttagaaac agtacctata
    48121 atatcatctg cctcataacc atctatttct agtctattta ttctaaattt atctaaaagg
    48181 tcttttaatg gctgtaattg ttctgccaat tcatctggca taccctttct tccagcctta
    48241 taatctttat attctaaatg cctaaatgta ggtgcttttc tgtcaaaagc tacacttata
    48301 tgtgttggtt tatatatatc tatcatttta aatagcattg tagtaaaccc atatatagca
    48361 ttggtcttta acccttcttt attattcatt tctggaagag cataaaaagc tctatttatt
    48421 attgaatttc catctattat aattaatttt ttttccaaat tatatcactc ctaaaaatta
    48481 tctatttttg taacatatat attatatacc aatatagtgt tatttaatat tcccataata
    48541 ataatagtat attaaaatta aatatgttgc aaacaatata tttttttgct ataataatat
    48601 acatgacatg cccgagtggt ggaattggta gacgcagtgg actcaaaatc caccggacta
    48661 atactccgtg ccggttcgag tccggcctcg ggcaccatta aaaactccta aagctattta
    48721 ttcaatagat taggagtttt tttatttttg atttaaattt taagttattt tttgaaatta
    48781 ctgtgtcttt ttaaaattta tataattata taaatttatc ttctagcatt aatttaaaaa
    48841 ctaatttact tttatataat tttctatttt atatcataac ttattacaac cttttatcta
    48901 atttattgta tacaacaaca ctacaaataa attgaaataa aataagcaat ccaagaacaa
    48961 ctaaaaccca cttgcttatt accattacag aaaatacact caatatagaa acaataaata
    49021 taagaattac aagaacatag cacatgattt gattagtaat acgtccagct ttatcacgta
    49081 acataattct tctttcatca ttcatctcta tcctttgatt ttttatcttt tcttcataat
    49141 ctactgctct ttcaggttta ctccaataca tatattgaag aatcatacat acacctggac
    49201 ctaaagcagc tccagataat ccccaaataa aagcctcaaa tgaaaactca gtaaatgtag
    49261 ccaataaaac aagtaaaatt ccacaaataa aatatataat tccaacatat aacatattct
    49321 ttttcatttt tactcctcct cataaataaa tatttcttca atactcatat caaagtatct
    49381 agctatctta aaggctaaga tgatagaagg attataccta ccattttcta atgaacctat
    49441 agtttgtctt gatacttgca aaatagaagc aagttcttct tgttttatgc ctctttcttt
    49501 tcttatttct tctaatctat ttttcacatc tatccccctt taatatctca actacggaaa
    49561 gtctactttc cataattata caactatatt ccatttatgt aaagcttact ttccatattt
    49621 acaaaaaaat aacagactat tttcagcctg taattttaga atttcatgtt gtttattaat
    49681 tttaatgtta tttattaatt ccatattatt tattaatttt aacttggttt ctgaattgga
    49741 atccaaactt ctgtagtata atcaggagca ttgatatcgc cattgggata aagttcaata
    49801 tctggtaaat tagcatacac atacccagaa cttggtaacc actcagtaat aattcttttt
    49861 tgcaattcct gtattgccag agcatttggc attggagaaa cacattcaaa tactgcccat
    49921 gtgcctgatg gtattaagta ttcatgagta tcttctaata caggcttatc tgttggagca
    49981 gcaatataat aatctaaacc cttactattt gtacacacac taactccaag caatccacaa
    50041 ggttcattat ttattaactc tgaaagttta tcaattgttc cattttttat gcatttagcc
    50101 cacaacttag gaactttctc aaagcactct tctacaatca tgttcatact ttcttttata
    50161 ccaacaatac gaaacccttt cttttctaca atacgatatt gcatttctat atttcctttt
    50221 acataaatgc ttaatgtcat cttaggatat gcttttaaaa caacaccctt tgctctagct
    50281 gcagatggag aaatgttatg tatattttga aaagctctat taaaagatgt tggtgaatcg
    50341 taaccatact tctcagatag ttctaaaatt ttgatgttac tgttctgaag ttcaaatgca
    50401 gccttagtca tacgtcttcg tctaatatat tcagataatg gtacttctgc aatatatgaa
    50461 aacattcttt gaaaatgaaa ggttgaacaa catgcaattt ttgctgcttc tgcataatcc
    50521 actccacttt cgagattttc ttcaatataa gtaatagctt gatttaattt atttatccat
    50581 tccatttgat attacctcca agcaaattat acagtttaat aaaataatat acctctctat
    50641 tcatgcacaa aattgtaagc atttaaattt ataagcattt ttcaatcaat acaataaata
    50701 taaaaattgt attatacaat aaaaaagttc aataacatta tattgtaaaa tgttattgaa
    50761 ctttttataa aaattaattt aagtgtagtt aaattaagta ttacactatc ttatatatta
    50821 caatatccta ctatcttata gtcttctggc actttataat ctttgtctgg agtacctaaa
    50881 ttccatttta aatcaaacat agttgcactt attattgttg caaaataaag cataactata
    50941 ccaatgtcta ttttttcttc atataaatta gtatgaccat cttttctaac agctaaaact
    51001 acagtgcctc catcaactat aaatctccat ggttgtctat ttaatgtaga tggagctaat
    51061 cttgcatagt gaaatgcatc aagaagtgct ctttcttcta agatttctat acttgcaaca
    51121 tttccccatt cgttcatata aacaacatct tctacaccta gtctagtaga ccccttatct
    51181 gttacaactt gcatatcaga ttttgaataa ttttgcccag tttttgtagc atttaatact
    51241 ttagcattac ttacattttc tccatatcca atagctataa ttcctgttac ctctttatct
    51301 gaattaattc caagtttttc ttttaccttt tttccatctt caaaagtaac ccaacaagaa
    51361 ccaattccaa gttctgttac tttaaagatt atactttctc ctatgtaccc tgaattttca
    51421 atgtaattat cttttatgtc agataatatt ataatataac ttggagaatc tatcatattt
    51481 cctttgtacc ctgctacttt atctaaatta ttaaaaactt cacttctatt cataattctt
    51541 acatcaacat ctatttctgg aactagtttc ttacaagatt ctgagtattt cttaatttca
    51601 ctaagatatt cttctttaat atctgtgttc ttatattctc taacagactt tctatttaat
    51661 atgattgttt tataatccat aattttatcc tccccaaact agtattgtct taattatata
    51721 cccattaata tattatttta ctcatttatt taataaataa agattacaat tttgtaatta
    51781 ttatttatta ataatattcc acttttacat taataatgac cagttgtgca aaatccattg
    51841 ccaaccacca aataaaaaca tattataaag gtaatataaa ataacaaaca aatcttaaca
    51901 aaaaattaaa ctttttttac acgaaagaag gcttcccgtt gccttcttta tttttataaa
    51961 aaaataagct gtttctaaaa tgatttcaca tttttgaaac agccaagtta ttctataatg
    52021 acaactataa tttgaggatg tgagggagag ttgtcattat aaatttaggg taattatatt
    52081 atgttttaag ctagttcttt ctatatttat ataatacatt ttatatatga attttatatg
    52141 agattggtat aaatgatata tgaaattaca tatgtttata ttatattaaa ttttattatt
    52201 acattttcca caacaatact catatgtctc cacattattt ggaatattta caaatacaaa
    52261 ttctttacag ttctcacaat actttaatat agatatagtt ccacaagtgt aacattcaaa
    52321 tattacacaa tcctcatttt cctctactat ttcaaacttt ttactatgtt caccacactc
    52381 tgggcacttg tgattattta cattcataaa tactcctcct tattagtata gttacatgtc
    52441 aaaaacttaa ttaatgtatt ctatattact tattatatat tctataataa atcatattct
    52501 aatataataa aacaaattaa ataaaaaaaa aaccataaat tttaaattta tggattaatt
    52561 cttgtaatga taactctatt tgaggatgtg agggagggtt atcattacaa tagggtatta
    52621 tattatggtt tttaagctag tctttctaca cctcatatga tacatatgct atatgaattt
    52681 tatatgattt ttacataaat attttatgaa atttaaattt tttagtcatt ttgaaatttg
    52741 taaaaagttc tctaaaaatc cacagcatca taaacagcca aaatttaagt aaaacatcat
    52801 aaaaaaattt gtccaataat tttaacaagt aatctcacct cattataatt attgacacaa
    52861 acttaaataa acaatctata aatcttctat aaatcttaaa ctttccccct attgatacac
    52921 aatttctagt ttgtcaattt tattttgcat ttctaagaaa atttcttcaa cagaattgtt
    52981 aaatatattg cctaaataga ttcttttatc tttttgaaat gaataagatt ttacactacc
    53041 atcagaagaa atatataaat gactccactc tgctccatat tttgaaaacc tctcctttgt
    53101 atctttaaac ctcatatctt ctatttttat tgctttttct ctgtctcctc ttactacagt
    53161 gtgacccctt tttattaaac tggttatttt tatatcttct tttaatttaa tacgtgtatc
    53221 atcatttttt gaatatactt tccatatcac tttatgacga taattatcta attcctttat
    53281 taagcttcta tcaatagtaa aactatcttt agttaccaca attaaatcaa aatggtctaa
    53341 catatattga aatatttctc tcatactttt attttttaaa atatcctcac caataacctc
    53401 tataaatcta cagcctttct catacatgca gttcaataaa cttgtgattt cagaaaaata
    53461 atcatttgta ttattatctc catctatttc tatagtaaag tacaaaggag taatgtatga
    53521 tttatttcct gttatctcaa atacacttga tagtgtctgt atatcatctt caaacgttaa
    53581 gtacacattc tcataaaaca taaaaaattc cataactttt tctttcatat tatggatatt
    53641 tctatatcta ttggataata tatttattat atcgtcagta gtatttttcc catcacattc
    53701 agccagtata acaatttgct tccaacttaa gctatccaca tttccgatac tttttttata
    53761 tccatcttct tcgtcataaa taggaccaat tataatgttt tcttttggcc acagatgaac
    53821 cctagttcca tcttttaatt tcggatattt attttttaca gactcataaa atctacttgt
    53881 atcacccatg attctttact accccccata gtttaaatat tctatgctaa aaatagcact
    53941 ttatttttat taattcaaca attattcaaa attggtcatt atatatgttt aaatggattt
    54001 aacatcaacc ttatttatat gttattgatg tttatatatg ttatataaaa tcttatttct
    54061 catacttaaa tgtaatctta actatctctg gtttacaatt aagcctaatt aaaacttttg
    54121 agcctcctaa gcctttattt aaaatcatag tgctattatc tttttcatat tttccccagt
    54181 catacttagg aaagaactct ctatttggag ataatacacc tccaacaaaa ggtattctta
    54241 ttatacctcc atgaacatgt cctgctaaaa ccaaatctgc tccccatttt gcatattttt
    54301 caaagaaaaa tggtgtatgt gctaaaagaa tgttatattc attttcatta actttaccta
    54361 gattgtcttc tacaaaattg tcacttagtt ctaatttttt gttattagca aataaatatg
    54421 gatagcattc caatggtata attaaaccat ataaattaag atggctatta cctctatata
    54481 ttcttacaga ttcattttcc atatttatta ttttctttga atatagttcc ttaaaataat
    54541 ccttatatag atgtttatac ttttttatta aagatttttg ttcatgattt ccaattatat
    54601 gatatacttc atatttttca accaaattat ctattaaatc taatgcaact tgaaaatttt
    54661 tattttctcc atctactaaa tctcctgtta taaatataac atctgggttt aatgattcaa
    54721 ttttatctat caatacctta ttatttttat caaacacctt attatgtaaa tcactcactt
    54781 gaactatttt aaagttatca aattcctttg gaattttttt gttgaaaata gtatattctg
    54841 tcgtttctat atgctgagtt tcatacttat acagaatata caataatatt aatactaata
    54901 ctgtcattga aacctttata atcatattct cttcctcttt attctatttt gttaatatta
    54961 ttataatgct tttatgtttt ttttaaaaca ataacatcat cacatacata taaatctttt
    55021 cgacattata aaaataagtt atccttttct aaaattttca tttattttag aaaaggataa
    55081 ctataaaatt atctataaac ctctatattg tgaaatatag tttttccttt atttaaattt
    55141 ttatatatat gctctaaagt tccatcaaac ctaatagaat gtccagactc taaaatataa
    55201 gttttcccat ccacattaag gtcaagttta ccttctatta cgagcacata ttcttctaaa
    55261 acatgactat gtgtagatga aacatgagta cagttttcct caagctcaat tataaaaagt
    55321 tcaaaccttc tttgagggct aaaagggtat attggatata acttcatgtt ccctttttgc
    55381 tcaacaatag gctctacttc ttctttcttt ataacctcat aggtatttgt attttcattt
    55441 aatagtgtag taaaatttat tttaaatcca gatgaaattt tccataaagt agatactgtc
    55501 ggacttgatt cacctctctc aatctgtcca agcattgctt tactgacacc tgtcatcttt
    55561 gaagctttgt ctaaacttat gcccatttct tgtctcaata accttaaatt ttctcccaca
    55621 ttaggaaatt tattatccat gttaaaatcc tcctgatagc tatttgtatt ctatgatttt
    55681 tactcctgta agttctacta tctcttttaa ttttaacata tgattgttac aaatatcatc
    55741 aaattcatat atataatctt caagccctaa tgccttatat ttatcgattc caaatttatg
    55801 atatggaagt aactccatct tgcctcctgg aacattttgt ttgacaaata atgcagtatt
    55861 tcttatattt tcttcactat catttactcc ataaataatt ggcactctta taaccataga
    55921 tttattcaac tttgaaagcc tacatatatt atctaatata attttattac ttactcctgt
    55981 atattcttta tgaatattat catccataga ttttatatct acaaatatgt ggtctatttt
    56041 ctcaaataca tcatttacct tattccaatc aaaatatcca caagtttcaa ttgcaatatt
    56101 tatgccttta tcataaaaaa tatcaactaa ttctcttaaa aaatcaatct gaagtgtagg
    56161 ttcacctcca gaaaaagtta tccctccatt agattcaaag aaaaatataa aatctttttc
    56221 aacttcctct actatttctt caacactcat ttcttcggtc attatacaaa tggcatcttt
    56281 aagacatacc ttgatacact ctccacataa atcacattta ttatttattt gcgctctatc
    56341 aaatagtgaa cttatatttt gaggacaaac atctatacat ctattacagg atacacattt
    56401 atctttcata acacctaatt ttactatgtt actccaggaa tctggattag aacaccactt
    56461 acattttaat ttacatcctt ctaaaaatat agtagtcctt attccatctc catcatttac
    56521 tgaaaaatgt tgtactttta caactctacc ttttttactc ataatctacc tcataattaa
    56581 aaattgctat gtgaatttct agctattata gcatcttgca tttctcttga taaattaaca
    56641 aattgagtac tatatccagc aaccctcact aataaatctt tatattcttc tggattttct
    56701 tgagctttta atagagtatc atttgatatc gtattaaatt gtacatggaa tcctcctaaa
    56761 gcaaaatatg atttaatcat agatgttaga tttctaagtc ctctttcagt ttccaccaaa
    56821 tcttgattca gtcttaaatt tagaagtgtt ccaccagtat gaacatcatg atttattttt
    56881 gatgcagatt tcattgctgc tagtggactt gttgtatcag taccaacaaa tggagaaact
    56941 ccctctgtca aaggttttgt cgcttttctt ccacaaggag ttgcacctac tatttttcca
    57001 gttggtacat agtttgatat acccataaat gcactattaa atttagagcc aaaaatatcc
    57061 ttgtattttc tagtttcagt ataataaaaa tcagaaactt caattgctaa tgaatctaca
    57121 taatcattat catttccata cttaggaaca tccaacgcaa gttttcttaa ttcttcataa
    57181 ccttcccaat cattatttag tgcttttgtt aattcttcta acgtagtcac cttatcctca
    57241 aatactaatt tttttatagc tgccaatgaa tttgaaacta cacctaaccc tataccagtt
    57301 aatacaggac ctatattata ttttgctcct cctttactta aatcaactcc cttatccaaa
    57361 catccatcta cacaagtaga taaaaatggt cttggtacca tttctttatg aatttgttga
    57421 gcaacagtag ttcctataac agaacttttt acaagatatg ctaattgctt taaaaactca
    57481 tccttaagtt cttcaaaaga agtaaattct gtgatatttt ttgtatctaa ccccattttt
    57541 tctccagtta atctactctt accttcattc atagcatatt ctaaagctgc tgcaaaattg
    57601 atatttacgg ctgaagtcca ctgtcctgtt tttctaaaat gaggaactac acaaccacag
    57661 ttactccaat ctctagcatc ttctgcatca tatccatctt gtaataacat ttgtgcccct
    57721 gcactatcgc tgtgtatagc agggaaacca gttccttgtt ttaccaattt agcaactgcc
    57781 attacgaaat tatctggtgc tccttgactc actctaacac ttaatcctgg ttggtgagtt
    57841 tttacacttt ctgtagcctt taaacacata tatgagatat cattcgttcc atcagtacca
    57901 tttctctttt taccaccaac agtaagattt tggaattgat tatatcctgc aaagtaattt
    57961 gctgtatttg ctgatatagt ccatacccat tctgataact taatccaaag tgcttctatc
    58021 aattcttgag cttctgattc agttatttta ccttctctag catcattttc ataatatgga
    58081 tacatatatt ggtcaaatct acctaaattt aatgccaatg gattttctga tagaatacca
    58141 cctaattgaa caaaccatac aaattgtatt gcttcataaa aattagttgg agggtttgct
    58201 ggtacattca tacatatctc tgaaattttt ataagttcta actttctctt ttcatctgtt
    58261 tcttgcttac tcatctcttt tgcaagattt gaatatcttt gtgccaatgt cataataccc
    58321 tgtgcaacta tagaaacaga tttgtaaaaa tctatctttt caatattttc tgaaactgat
    58381 atatctaatt ctttaatttt tttatctgca tcttccttaa tccctttata tccttttaca
    58441 aacaatatat cttgataatc tggtgttact tcaccaactg cctgtctcca cttagaatca
    58501 ttatctatta ttcctgtatc tactaatatc ttagctgtat cttctgggat tcttgctaag
    58561 aaaacttctt ccaaagattt tcccttccaa tatggaaata tttcttttct tataaattct
    58621 atttgttctt ttgatattac atatgggtct tgagttcttt tatcaaaagt atccatttct
    58681 ttgtctaccc attgccaaga aaactctggt gtcaatatcc ctgttcttct aaattttcct
    58741 gctgtaccta ctattaattc attttcaaag atttttatag gaagttcttt acatgtctcc
    58801 aaaaaagctt tagctcttct tatacagata gcttctccct cagttttttt atgtgattct
    58861 gtaaaaattc tagccctttc acaacatatc ataggtttag aattaatata ttcttgtcta
    58921 aatgcttcta ttctattagt ttgactcatt taaaatcccc ctttggataa tatatactac
    58981 atttttgtat gttataacat actttaatta tattatactg tcctttagca taaaatggaa
    59041 gcaaaataac ttattttttt tcatctacct tgaacaccta tatattaata ctttttttgt
    59101 taaatttata aactctaaaa aaattttcta tgatatattt tctatattgt tatagttatt
    59161 tctaatatac taatgatttg aaatatcaaa tttgtatact agacttagtt ttaaaacctt
    59221 agttgattgt ttaactttgt taacttcaac atgtatatcc ttcatataag gaagctctac
    59281 aacacctact cccttattaa agtcttcttc aaccatagaa aaaatttctt tctcagttaa
    59341 gttcgtatta gtcaatgact taaataaatt gtatataaat ttaatatatt tatcactctc
    59401 agataactta tcatcttctg gatactcttt agcacagaga atatctattg ttacttttcc
    59461 ttctttatcg tcttcagaca tagaatagaa taaacttcga atattttttt caggtggttt
    59521 ttcagcacca tttatagata ctacaaagct accatctgat tttgagtcag taatactata
    59581 accactgtct attatagctt tcgaaaatct ttgagataaa tcttttttta cttcttctct
    59641 acctttcgca tcattaatct tttgtgagta attattaaaa agctcgatag attcatcttt
    59701 tttatcttca acttgttctt tcaacgaatc tttactaatt tgttctgagc tacttaagtt
    59761 cttatcatat acaggagaca tacaccctac catcaatgct aagctaagat ttaaagccac
    59821 aatgattgcc actctattta tattcctttt tactttcata cacaatcctc ccatcacact
    59881 aaccgctcta aagtattact tattgcacta gaatatgttt atattttctt tatattaaag
    59941 atttattatt caatacatat caccttccta tccaattctt ataacttact attagtaaaa
    60001 ttataagctc cttaatacag aaataaggat gttatctttt gttattaaaa gataacatcc
    60061 ttattttttt gtatcaatac atatgtccca actggtaact taatcgcccc aactgtcagt
    60121 atcttggtcc aatggtatat atatatttag tataaattta tcgatactat tttcaaataa
    60181 taattctcca ttatattttt gtaaagaaga tttaatactc tgaataccta ttccatgtac
    60241 aaacttatcc attttgtttg ttaacaatat atttttatta aaagtaagct gatttagttt
    60301 actattttca catttaagta caaaaaatga ccttgtcata gttcctttaa ttctaatata
    60361 tttattaaca ttttcatcca ctattttatc acatgcttct attgcattat ccaaaatatt
    60421 agaaaatatg cttgacacat ctataggttt tacaaaattt acttttgaaa aatttataga
    60481 gcaagtaaat ttaattcctt tttttaaaca tatttcactc ttttcgttga ttataatgtc
    60541 taatatcata tttcctgtat tatagatata tttaaatgtt tttatttcat cttttaaatt
    60601 gttaacatat tcattaattt ctttgctatt gtttctaagg ttgtctatac aataaatgtg
    60661 attatttata tcgtgatata gtttcttaac tcgcatatga gattcttgaa tacttaaata
    60721 atgcgcatat tgcatatcta acttattttt tattaattca gcttgagatt ttaatctatt
    60781 ctcttttatt atttctttaa acaatagtat aagaaatata ctactagctc ctaccaattt
    60841 tggattaaca aaattatcaa aatagtatcc ttctgaatgt aatgacccaa accgttctaa
    60901 tttttttaat acataattta caagcatatt cattgtatta actaataagg caaataataa
    60961 atatacataa tttctcttat cttggctaat taattttata gacttcctaa aaaagctaaa
    61021 tataagatat ataagaataa ttattaaatt actatatatc tgaattttta tagattctaa
    61081 gtgaggtact aataaggatt ctttagatga aataacatta cctgtaattg caaaaaatag
    61141 tattctatat ataacagcat ccaaacattt ataatataca ctaaatagat aaaagaaaat
    61201 ggaatactta atataaccca tcttataaaa ataattatag tatcctcccc aagcaataaa
    61261 tgctaatata taaaataaat agtttatatt actaaagtaa tttcctccat taaataatct
    61321 acttatctga attataatat aaataagaaa caagacaata tagaatacta ttttttcatt
    61381 ttttttacta tcctttttat aaattaaaat tccgttcatt atagaaaact gtataaatat
    61441 agataaaact actaatagct cagtcataaa gtttagcata ttgtatcacc aagaaatttt
    61501 aagaatttct ccttaacttc tttatatcta tatctactaa taggtacctc ttctccactt
    61561 tccagtattg cagtattagg ctttatattt tcaacatatc ttaaatttac tataaaactt
    61621 ttatggcatc ttataaattt atatggattt aattcttttt ctattttgcc taaactatat
    61681 tttatatcaa aattcttgtt tattgtgtgt attagcatat cttttttttg aacttctata
    61741 tattttattt catttaaata aatcttatat gtattagact tattttttat tgtaatatga
    61801 ctttccttat ttatttcaat atctttgata caagttagca catgtttttt tagttcttcc
    61861 aactctattg gctttagtaa atacctataa gctcttacct cataaccttc ttgcacataa
    61921 tctattaatg aagttacaaa tattatttcc atttcattgt ctacttctct aatctttctt
    61981 gcagtatcca ttccatttaa tttttccatt tttatatcaa gaagtaatat atctatatct
    62041 tttaaattac tttccagtaa ctcttctcca gatatatact tttgtatctc atattctata
    62101 cttatatctt ttaatatatt ctcaaggtaa tcttctatta aaagttgtgt ttctttttca
    62161 tcttcacaaa ctgctatctt aatcataaaa tgcctcctta cataatttct agttaataca
    62221 attaaaatat ttataatata tttaaagata attttgtata tctgatactt taggataaca
    62281 ttttcaaatt atttttatca atactcaatc ccatataact attatttgat atttcaattt
    62341 aaagacagaa attactataa ttaaaaacag tacttactga taaaagaaga tgtcttaaac
    62401 tattaaaaaa ataattttag gacaccttct tttatttatt taaacttttt aagccttaaa
    62461 taatacatta atctcctata taattgtact gcatataaaa tcaacaatat aatctgtagt
    62521 acaatagcag ttacagatgc tccataacca ctatataaat ttcttatttc aatattcaat
    62581 aaatatataa atattactac taacataaag aatataaaca tattcataaa taaaattgac
    62641 tttactttgt tttttttatc agaatcacta tataatttag gaggtatgtc tttgtattct
    62701 ttcgaccata tataccatgc agatgtaccc ataaaactac caatactcct aaatacaaga
    62761 ttccagccat ttgatttatg ttcatcaaaa taattattct ctacatcata gtagtaatca
    62821 acagtgtatt ttatttttct tggacttccc ttatgaaaat aaaatattcc tccaaattta
    62881 tttatcttat ataaattata tcctttagct tcaatatctt ctaaccattt ctctatctta
    62941 tcaggtgaac tataccagtc aaatttgaat tttattttct tttccatagc actcattctc
    63001 caaaatgata tttttaaata gcttaaaaat ctatttaaat aattatatca ttttgatact
    63061 aattttaggt tacaatcata tatttaaaaa gctatttaaa tagtaataaa ctaatagcca
    63121 taacacccat tcctgcaatc aatccataaa tagataaatg atgttccccg tactctctag
    63181 ctgatggcaa taattcatct agtgatatga ataccataat tcctcctaca attgcaaata
    63241 caattccaag agttatatca tttaaaaaat ttctaagaag tacatatcca ataatcgctc
    63301 ctaaaggctc agacatacca gataaaaagg aataataaaa agctttcttt ttatctcctg
    63361 tggcataata aactggtaca gatacagata taccttctgg tatattgtgt attgcaattg
    63421 caattgctat cggaatagct attgtaacat catcaagagc tgatataaat gtagctaatc
    63481 cttcaggaaa attgtgaatt gctatagcca ttgctgtaaa tatacctgtt ctcaaaagag
    63541 acttattttt tttgatattc ttttcttcta tattctccat tttctttatt tcatgtggat
    63601 tttcttcttg tggaattagc ttatcaataa cagcaatgat agccatacct ataaaaaaag
    63661 ctattacagt tgaccaactt cctaatttct ctcccataga ttttgtcaaa gcatcttgtg
    63721 ctttaggaaa aatttctatc atagaaacat aaatcataac accagcagaa aatcctaagc
    63781 ttatagaaag aaacttagta tttgtttttt tagcaaaaaa tgctatacaa cttcctatac
    63841 cagtagataa accagccaat aatgttatca aaaatgcaaa tattacatta ttcaaataaa
    63901 tcaccttctt tttaattgat attgattatc attttcttta tgctatcata tatattttat
    63961 atagtcaata tttataaaaa atatacacaa taaaaaagca gagatataaa cttagtctct
    64021 gctttttctt tatagtatat taaaattatt tatttaagaa atccactgca tattgcacat
    64081 ataaagcagt tccaatttct agagcatctt catctatatt aaagcatcca tgatgatgag
    64141 gataacaagc accttttgac tcatttctag ctcctacaaa tgctagtgca ccaggtgcta
    64201 tattcataaa ctcagctaaa tcttctgctc ctgttacttt ttcaaatagt gttatacagt
    64261 cttctccaaa tagcttaatt gctgcatctg tagctatctt tgaacattct ttgtcattta
    64321 taactgctgg tgttaagtac ccatactcta attcagcatc tgctctatat gcttctgctg
    64381 tagattttgc tatacgttct aaaattccag gaatttgctt tctaagctct ggattaaata
    64441 gccttattgt accttctaag acagcttcac tcgcaattac gttaaatcta gttccagaat
    64501 ttaaaactcc aacactcaca actaatggtt ccagaggact tacttctcta ctaaccatag
    64561 attgtagatt cataactatt gctgaacttg caagcactgc atcaacacct tgatgaggaa
    64621 gtgaaccatg accacctcta ccttttacag taattttaaa gaaatcagca gaagccattc
    64681 ttggtccttc ttctaccgat atggtcccag attctacatc agtccaaaga tgtattccaa
    64741 acacactatc tacaccttcc atagcaccat cttgaatcat agctcttgca cctttaccaa
    64801 cttcttctcc tggctgaaaa aataatttta cagttccatt gatactatcc ttaatatcat
    64861 ttaacacttt tgctgcccct agaagcattg atgtgtgacc atcatgacca caagcatgca
    64921 taagtccttc atttttagat ttgtattcta catcagtgca ttcaacaact tgtagagcat
    64981 ccatatcacc cctaagtgct acagtctttc ctggattagc tccttttata gttgcaatta
    65041 cacctgtacc tccagctgat acatatggta tccctatttt atcaagttct tctttaactc
    65101 tctttgatgt tctcacttct tccatacttt tctcaggatt ttcatgaaat tcccttctaa
    65161 gcttaattac atagtcttta taactctttg ttatttcttt aatatccatt ccatacctcc
    65221 aagtagtaca cattcttctt atttaaaata ctttttatca cttaaatact atattagatt
    65281 tacaaagata cctgctatta ctacagaagc tattgtaact gtagtaaatc ctccaacaag
    65341 catctttgga agcatcttat ccattaaaaa ttctttttct tctggtgttt cagccaaagc
    65401 ctttactgcc tcttcagtta atatatagtt tggtggaaaa ccataaagtg atgtaagcga
    65461 aactgaaaaa gccatttctt tactttcacc taaaagctta cctactataa tagagaatat
    65521 tcccatacct attacaccaa atattatgat tcctacaaga ggaactgcca tttcaacaag
    65581 cattgctggt gtagcttttg ctaaccctgc aaatataaaa gccatcaatg atgtcattaa
    65641 ccatccaaat gaaccagatt tattaagtgg ttttctatca actattccaa gttctgatgc
    65701 tattacacca aatattaaac atataacata tcttgataca acttcatttg taagatttgt
    65761 aaattgtact gctaaccatg ctacaaatcc taatctaagt attatcatat atgtcgtatc
    65821 atatttttct ggtaagtctg gtattatctt aaatctgctt ttttgttcag gtaaatcctt
    65881 aactttttct tcatctgtat ttacagttac ttttccactt ctaaagtctg ataaaagtct
    65941 ttttccttct tttttaagca ttaatgctgt aataggataa cccgcaaatc cttgtgcaac
    66001 atacatcaaa attgcaagta ctgataaact tgcaagacct tttgctgtag cagcatctgc
    66061 catcattata gatgcaacta gccctccagt aagaggaggt gtagctatta ctgctgtatc
    66121 ccatccaaat actactgtcc ctaatgccaa agcacctaca catattccta ctattcctgc
    66181 caaggctatt actattgttt tccattgacc tgctaattcc ttaatactca tcatagttcc
    66241 catatgagtt attaacaaaa gcatacctaa ttgagctaat ggtgttccta aagcggctaa
    66301 gtccacaata ttttctggga aaaaagtcca aaatcctact ataaatataa ttgctgatac
    66361 aaaaaccgac ggaacaaaag ctttagtttt tgctgaaact atatctccta tcgtcagtga
    66421 tactaaaact agacaaaaag ctgttattgc ctccatacaa aatcccccct cataataaat
    66481 atatttttat aaaatgtatg tatagttatc cattatttta ctacttttga aaaacatttc
    66541 aagtcaaaat tcgatttttt tcataaaatt cgataaattc cataaattaa agttagtttt
    66601 ctaaattatt ctttttgtaa aaacgttatc ccgaccattg gctatttaac tctattctta
    66661 caaagagaat taaatgtaat ttgcaaaaaa taaaacatag gatacatttt attcataaaa
    66721 catcttataa atataaaatg aattgtcttt atgaatctta atatatccta tgtttgtaag
    66781 tttaatgtgt atgttagcta tggtgtaaaa tttgtatact agttattgtg taacctacta
    66841 tatactccat ttttgtttac tagctcttca tgagtacctc tttcttcaat tcctttatcc
    66901 gtcaatacta tgatttcatc agcatttttt atagtagtca atctatgtgc aacaactaaa
    66961 tttgttctat ctttacttaa ttcctctaaa gactcttgaa tttctctttc tgttacattg
    67021 tctaaagcag atgtagcctc atccaatatt attattggag gattctttaa aaagattctt
    67081 gaaattgaaa ttctttgttt ttgcccacct gataatttta cgcctctttc tcctatataa
    67141 gtatcatatc catctggcaa agtctctaca aagtcatgta ttctagcttt tttagcagca
    67201 gctatcattt cttcatctgt ggcattaggg ttcccacaaa gaatattatc tcttatagtt
    67261 cctgtaaata aaaatacatc ttgttgaact atacctatat tttttctcaa tgattttaaa
    67321 ctaacatctt ttatactctt tccatcaatt tttatatcac ccttatcaaa ttcataaaat
    67381 cttggcaata aattacataa tgtagtcttt cctcctccag aaggtccaac taatgctata
    67441 gtcttaccag cttttataga taaacttaaa ttatccaaaa cttgagtctt atcttcatat
    67501 gtgaaagata tattttctat ctcaatgtca ccttttacat tttcaagttc aataggattt
    67561 ttagcttctt tttgatggtc ttgttccatt atctctatga atcttttaaa acctgtcata
    67621 ccattttgat attgctctgt aaagtttatt aattttttca taggttgtat aaacattttt
    67681 acatatagta tataagcagc aaaatcaccc aaagtaatct ttcctatata agtaaaataa
    67741 ccaccagcta ttacaactac ccactctaag atatccatgt ataaattcat accagagtaa
    67801 tattctgcca ttaccttata agaatcttgt cttgctcttc taaataattt attgcttctt
    67861 acaaacttat ttaactcttc tttctcagta caaaaagact tagttacctt catacctgct
    67921 atactatttt ctaaagtagc atttacatca cctatagtaa ctctactctt cataaaagca
    67981 atattcatct tatctttttt agctattgca aaccaaatta taaacggtaa tatagcaaat
    68041 attataagcg taagaggaat atttatatct ataagtataa agaatgaacc aaccaacatt
    68101 actatagaaa tgaataaatc ttcaggccca tgatgtgcaa gttctgttat atccattaaa
    68161 tcatttacta ttctagacat agttactcca gatttgttat tatcaaaata cgtatttgga
    68221 agtctttgta agtgtgtaaa tacctcattt ctcatatcag cttgcatatc tacaccaata
    68281 acatgtcccc aatactgcat aaaatagtta agccctgctt ttgctataaa tataagtata
    68341 agtgctactg caaaaactac taacattctt aagtttttat ttggaacaac gtcatccatt
    68401 atatttctag ttatcatagg ataaactaaa tcacataagg aaaatagaaa agctgctata
    68461 aggtctagtg taaaaatttt cttatatggc ttataatatt tgacaaattg ttttatcata
    68521 agtattaaat ccctccttta ttagtacttc tattagtact tctaaaaaat acccataaat
    68581 tagtataaca taacaataac tttaaatctt ttataaactt acaaagtaat aacaatcttt
    68641 ttcataaaat tgaagattta tatcatatag ttgtatactt gaaacaacat aaaaagagct
    68701 atatctctat atttaaaatt ttataaagat acaactctat atttaatact aaattttttt
    68761 tataaataca aaactacttc aatgcattta atatcttttt atctaatgct gttgtattta
    68821 tacctgttat atatcctgtt gcctttattg taggtatatc ataagttgtt ggtagtatag
    68881 tagtagatac gattaaatca gcaccttctt gttttgttga acattcagca attttacatt
    68941 gctcaacttc tgcttgtata ttattttctt taaccaatct ttctactctg tcacaaacta
    69001 ttgttgatgt tgcgattcct gctccacagg ctactaatat ttttttcata tattgtcctc
    69061 ctacaatttc tttaatattt tataactcaa aataaaattg ataaatttct tatatcttta
    69121 tttatataaa taattatatc attttatata agaagaacat cttaaaatta ttcttttaat
    69181 atgtttttta tttcaacatt gcttatactt aaaatttcat tttttataca agactctatg
    69241 tactttgata tatttttata atcatttaaa ttaaactgtt caatattcaa tttatattct
    69301 atatcactaa caatacctat caaatcagac ttattagata aaatatcctc atacttacct
    69361 tttctagtta cttccagtct tttaaatcta taatttttat atccttctat gataatcaag
    69421 tccatatctt catataattg aatcatttta tataatggat tcttctcttt tagttcttca
    69481 atatatgcta ctcgtttatc tgatattata cagacttttc tcgccccaga ttttctatgt
    69541 ttataagtat cttttccttc ctcatccata tcaaaatcat gacaggtatg tttaataact
    69601 ccaacacgat accctttttt atttaattct tttattatgc cttcaatcaa cgtagtttta
    69661 cctactcctg aatatgagac aactgacaat atattataca taaaattgtg tcctttcata
    69721 aaactatcta ataacaacat ccactaattg attttctttt attgtctcat tctttttaat
    69781 cttaatcata caattacttc ttagagttgc agatagtcta gcttttgcat ctttcttatc
    69841 atttagctct acatacaatt taccttcact tgtatatagt tttccactta caaatctatc
    69901 tttatttgaa aatttattaa attctccttt aaatatagct tttatatgat gtgttccctt
    69961 cttatatgtt aaatactcaa taacaggaac tatcacatta tcaaatacca ctattgctgc
    70021 atgtggatta ccagaaagac caaataatag cttttctccc aatgtaccta cataacaagc
    70081 tccaccaggt ttaatgttta tcttccaaaa taattctgtt gcccctattt ttttgaatac
    70141 atctggaatt aaatctttat ctccaacaga aacaccacct gtggtaatta aaaaatctac
    70201 actctgtaac atattcttta cctttttttc taactccaat atatcatcag aacaattgca
    70261 tattgtatct gcttgtattc caagttttaa taatcttgaa tataaggtat atctattact
    70321 atcgtatatt ttaccattct ctaaagtctc atttatatct tgtagttctg tacctgtatt
    70381 caaaattcca acttttaaat catcataaac agttactttg gatattccaa gacttgctaa
    70441 tactccaata ccatcatatc ctatattctg atttttatta ataatcaatt gccccttttt
    70501 tatatcctca cccttaaata tgtaattatc gtattccttt aattttatat ttaaagttat
    70561 catatcacct ataactgtta catcttcttt ttttattaca caatttgcac cctgtggtat
    70621 ctttccacca gtcattatct tcactgcttc atttttattt aataccctat taaatacatc
    70681 tcctgcatac aaagtcccta taacctttaa atttctattt tcagaatttg cattatatgc
    70741 ataaccatca tagggtgacc tatcaaatgg aggactatca attgttgaat atatatcttc
    70801 tgcaactacc ttatttactg catctataag atttactatc ttgaccctgt ttattggtaa
    70861 aacatttttt tcaataattt ttatggcatc ttctaattct atcattttaa atactcccca
    70921 atatattttt tatttaagat atttctatgt aatatattat agattataaa agttggaaat
    70981 acttttatct tatatttaat tataactaat agttatctat aagtgcagtt caaattttat
    71041 aaatctaata aaaaaactgt tcccagctag aaatttcctt ctatcttgaa acagcatcat
    71101 atgctttaga atattttatt ctaataaagt tccttttatt atttttacaa ttttatcact
    71161 ttcaatagtt tttatagatt ttattttgtt atccacaata cttattgtaa gtacctcttt
    71221 ttttaatatt ttgttatctt ctttcaatct ataactctta actactttac tgccttcatg
    71281 attatatgct gttaaatcat aattcgcatc tcctacaatt tcatttattc tggaatcttc
    71341 aacaccaaca tactctccca ggtcaatatg tctaaaattt tctagtgata cagccgattt
    71401 ttcatggtca cttttttcaa tcactatact agattcagca tcaatgactg atataccttg
    71461 tgatgagaaa gaattggttt caacattgac aactttattt tcgttaatat caacatataa
    71521 agcacttcca tctagttcat catttccagt atattttgga taaactaatg tcatataact
    71581 tgatgtattt atatcttgca ttgtatttga ttcttttaaa actttaggtt ttatataata
    71641 aaatgtatta taaggtatac ccatattctt tataatgtac ttataatctt tattcataat
    71701 ttcattaaca tcattttgaa cttttgtcat agttttttgc tgtgttgctt tacttgctac
    71761 ttgatttttc tctttagtat tttctttatg acatccaaca agtataatac ctaaaatagt
    71821 tacacaaact aatgaccttt ttaacttcat aaatcaatcc tcctttctta aattttatag
    71881 tatttatcta tatctcaatt tacagttatc tcttttttca ataa
//
LOCUS       DAMJWS010000008        70182 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMJWS010000008 DAMJWS010000000
VERSION     DAMJWS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 70182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.008"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            212..1429
                     /locus_tag="PC399_000560"
     CDS             212..1429
                     /locus_tag="PC399_000560"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF2591438.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1524..1904
                     /locus_tag="PC399_000561"
     CDS             1524..1904
                     /locus_tag="PC399_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2591439.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2188..2670
                     /locus_tag="PC399_000562"
     CDS             2188..2670
                     /locus_tag="PC399_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2591440.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2691..3506
                     /locus_tag="PC399_000563"
     CDS             2691..3506
                     /locus_tag="PC399_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2591441.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3559..5109
                     /locus_tag="PC399_000564"
     CDS             3559..5109
                     /locus_tag="PC399_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2591442.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGYIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSVIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5206..6558
                     /locus_tag="PC399_000565"
     CDS             5206..6558
                     /locus_tag="PC399_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2591443.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6689..7447
                     /locus_tag="PC399_000566"
     CDS             6689..7447
                     /locus_tag="PC399_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2591444.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLISNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELFNLLHTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7494..8213
                     /locus_tag="PC399_000567"
     CDS             7494..8213
                     /locus_tag="PC399_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2591445.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8337..9737)
                     /locus_tag="PC399_000568"
     CDS             complement(8337..9737)
                     /locus_tag="PC399_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF2591446.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9741..10649)
                     /locus_tag="PC399_000569"
     CDS             complement(9741..10649)
                     /locus_tag="PC399_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2591447.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPASEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKMIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10910..11557
                     /locus_tag="PC399_000570"
     CDS             10910..11557
                     /locus_tag="PC399_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2591448.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11881..14520
                     /locus_tag="PC399_000571"
     CDS             11881..14520
                     /locus_tag="PC399_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2591449.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14578..15681)
                     /locus_tag="PC399_000572"
     CDS             complement(14578..15681)
                     /locus_tag="PC399_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF2591450.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIHLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16042..16863
                     /locus_tag="PC399_000573"
     CDS             16042..16863
                     /locus_tag="PC399_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2591451.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16942..17153
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17240..18556
                     /gene="argH"
                     /locus_tag="PC399_000574"
     CDS             17240..18556
                     /gene="argH"
                     /locus_tag="PC399_000574"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF2591452.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18805..19143
                     /locus_tag="PC399_000575"
     CDS             18805..19143
                     /locus_tag="PC399_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF2591453.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19270..20559)
                     /locus_tag="PC399_000576"
     CDS             complement(19270..20559)
                     /locus_tag="PC399_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2591454.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20639..21343
                     /locus_tag="PC399_000577"
     CDS             20639..21343
                     /locus_tag="PC399_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDF2591455.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQRNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21435..22481
                     /gene="selD"
                     /locus_tag="PC399_000578"
     CDS             21435..22481
                     /gene="selD"
                     /locus_tag="PC399_000578"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21483..21485,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF2591456.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22499..23911
                     /locus_tag="PC399_000579"
     CDS             22499..23911
                     /locus_tag="PC399_000579"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF2591457.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            24025..25908
                     /gene="selB"
                     /locus_tag="PC399_000580"
     CDS             24025..25908
                     /gene="selB"
                     /locus_tag="PC399_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF2591458.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            26013..28751
                     /locus_tag="PC399_000581"
     CDS             26013..28751
                     /locus_tag="PC399_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF2591459.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28870..29814)
                     /gene="manA"
                     /locus_tag="PC399_000582"
     CDS             complement(28870..29814)
                     /gene="manA"
                     /locus_tag="PC399_000582"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF2591460.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29987..31348
                     /locus_tag="PC399_000583"
     CDS             29987..31348
                     /locus_tag="PC399_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2591461.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31436..33325
                     /locus_tag="PC399_000584"
     CDS             31436..33325
                     /locus_tag="PC399_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2591462.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33340..33795
                     /locus_tag="PC399_000585"
     CDS             33340..33795
                     /locus_tag="PC399_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2591463.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33854..34171
                     /locus_tag="PC399_000586"
     CDS             33854..34171
                     /locus_tag="PC399_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2591464.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34196..35458
                     /locus_tag="PC399_000587"
     CDS             34196..35458
                     /locus_tag="PC399_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2591465.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35563..36627
                     /locus_tag="PC399_000588"
     CDS             35563..36627
                     /locus_tag="PC399_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2591466.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36681..37859
                     /locus_tag="PC399_000589"
     CDS             36681..37859
                     /locus_tag="PC399_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2591467.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(38050..39108)
                     /locus_tag="PC399_000590"
     CDS             complement(38050..39108)
                     /locus_tag="PC399_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2591468.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39175..39546
                     /locus_tag="PC399_000591"
     CDS             39175..39546
                     /locus_tag="PC399_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF2591469.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39583..40257
                     /locus_tag="PC399_000592"
     CDS             39583..40257
                     /locus_tag="PC399_000592"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF2591470.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40506..41345
                     /locus_tag="PC399_000593"
     CDS             40506..41345
                     /locus_tag="PC399_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2591471.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41589..41685
                     /locus_tag="PC399_000594"
     tRNA            41589..41685
                     /locus_tag="PC399_000594"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41623..41625,aa:Sec,seq:tca)
     gene            41840..42547
                     /locus_tag="PC399_000595"
     CDS             41840..42547
                     /locus_tag="PC399_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF2591472.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(42689..43525)
                     /locus_tag="PC399_000596"
     CDS             complement(42689..43525)
                     /locus_tag="PC399_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2591473.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSKAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            43713..44618
                     /locus_tag="PC399_000597"
     CDS             43713..44618
                     /locus_tag="PC399_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF2591474.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            44627..45811
                     /locus_tag="PC399_000598"
     CDS             44627..45811
                     /locus_tag="PC399_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2591475.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            45926..47227
                     /locus_tag="PC399_000599"
     CDS             45926..47227
                     /locus_tag="PC399_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2591476.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            47181..48224
                     /gene="holA"
                     /locus_tag="PC399_000600"
     CDS             47181..48224
                     /gene="holA"
                     /locus_tag="PC399_000600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2591477.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(48411..48677)
                     /gene="rpsT"
                     /locus_tag="PC399_000601"
     CDS             complement(48411..48677)
                     /gene="rpsT"
                     /locus_tag="PC399_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF2591478.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            49273..49794
                     /locus_tag="PC399_000602"
     CDS             49273..49794
                     /locus_tag="PC399_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2591479.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            49828..50604
                     /locus_tag="PC399_000603"
     CDS             49828..50604
                     /locus_tag="PC399_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2591480.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            50946..51899
                     /locus_tag="PC399_000604"
     CDS             50946..51899
                     /locus_tag="PC399_000604"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF2591481.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            51917..52936
                     /locus_tag="PC399_000605"
     CDS             51917..52936
                     /locus_tag="PC399_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF2591482.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            52967..53218
                     /locus_tag="PC399_000606"
     CDS             52967..53218
                     /locus_tag="PC399_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591483.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            53500..55305
                     /gene="lepA"
                     /locus_tag="PC399_000607"
     CDS             53500..55305
                     /gene="lepA"
                     /locus_tag="PC399_000607"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF2591484.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            55454..56791
                     /locus_tag="PC399_000608"
     CDS             55454..56791
                     /locus_tag="PC399_000608"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF2591485.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            57024..58337
                     /locus_tag="PC399_000609"
     CDS             57024..58337
                     /locus_tag="PC399_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2591486.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            58509..59684
                     /locus_tag="PC399_000610"
     CDS             58509..59684
                     /locus_tag="PC399_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF2591487.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            59787..60806
                     /gene="hrcA"
                     /locus_tag="PC399_000611"
     CDS             59787..60806
                     /gene="hrcA"
                     /locus_tag="PC399_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF2591488.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            60836..61456
                     /gene="grpE"
                     /locus_tag="PC399_000612"
     CDS             60836..61456
                     /gene="grpE"
                     /locus_tag="PC399_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF2591489.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            61548..63395
                     /gene="dnaK"
                     /locus_tag="PC399_000613"
     CDS             61548..63395
                     /gene="dnaK"
                     /locus_tag="PC399_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF2591490.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSESEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            63606..64760
                     /gene="dnaJ"
                     /locus_tag="PC399_000614"
     CDS             63606..64760
                     /gene="dnaJ"
                     /locus_tag="PC399_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF2591491.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEATFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            65726..66661
                     /locus_tag="PC399_000615"
     CDS             65726..66661
                     /locus_tag="PC399_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2591492.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKKRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            66697..68070
                     /locus_tag="PC399_000616"
     CDS             66697..68070
                     /locus_tag="PC399_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2591493.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(68258..69025)
                     /locus_tag="PC399_000617"
     CDS             complement(68258..69025)
                     /locus_tag="PC399_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2591494.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(69055..70164)
                     /gene="ugpC"
                     /locus_tag="PC399_000618"
     CDS             complement(69055..70164)
                     /gene="ugpC"
                     /locus_tag="PC399_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF2591495.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT        27515 a         7678 c        12088 g        22901 t
ORIGIN      
        1 agaatgtaaa aaatattttt aaattatcat ttaatgtaat ttaaatgaat tgaagatata
       61 tgtttattgt atgcaattgt tgtattttta ggttttttgt gattaattaa aaaattataa
      121 aaatcgttta catgttgatt tttaaaaaaa aaagatatag aattatatag aaagtaataa
      181 acaaaaattg gatttacggg agtggaatga aatgatgaat gttaccttaa cagatgtaaa
      241 agaggctaga gagaccatta agaacatagt aaaaagaact gacttacttg aaagtgttaa
      301 acttagcgaa aaaacaggag caaatgtgtt ctataaatgt gagaatcttc aaaaaacagg
      361 ttcttttaaa ttaagaggag cttgtaacaa aatagcgagt ttaacagatg aagaaaaggc
      421 aaatggagtt atagcttcaa gtgcagggaa tcatgctcaa ggagttgcat tgggagcaaa
      481 aatgactggt ataaaatcta caatagtaat gccagcaact gcgcctttag caaaagttag
      541 tgcaactaaa ggttatggag cagaagtagt tttaaatggt gcggtttatg atgatgcata
      601 tgcaaaagct gttgaaatac aaaaagaaac aggagcaact ttcttacatc cattcaatga
      661 taagtatgtt atagcaggac aaggaactat atctcttgaa attttcgaac aattagacaa
      721 taaagttgat actatactat gcccagttgg tggaggagga atcatctctg gtgttgctgt
      781 tgcagcaaaa gcattaaatc ctaatgtaaa aatagtcggt gttcaaactg caaatatacc
      841 ttcaatgaaa gagtcaataa aaaatggaaa agtaacaaca gcatttaatg atactacaat
      901 agctgatggt atagctgtta aaactccagg agatttaact tttgaaataa taaatgaatt
      961 ggttgatgaa atagtagtgg ttgaagagac agaaattgca gaatcaatat tatttatgat
     1021 ggaaagtcaa aaaatagttt cagaaggtgc tggtgctgta tgtacagctg ctatattaag
     1081 tggtaagtat gtaccagcaa aagatgaaaa tgtagtttgt ataatatctg gtggtaatat
     1141 agacataaat acgttatata gaataatagg tgttgcttta gctaaagaag gaagaagata
     1201 ttcatttagt acaattatgg aagataagcc aggaaacttt gctgagttaa ctagaataat
     1261 aagtgagaat ggtggaaaca ttttaagtgc taatcaaggt aaattatcag caggggaagc
     1321 tctaggaaaa caaagtgcgg aatttatatt agagacaata gattatgacc atatagctag
     1381 aataaagaaa gctatagaag aaaaaggttt taaaattata gaattataat aaaaagtgta
     1441 tataatatac aagaaaaata gtataatagt gtatgaagat acattttatg tgtattagat
     1501 taacgaatta ggaggatttt aaaatgaaac atgaagtaat acatactaat gatgcaccag
     1561 cagctttagg accatattct caagctataa aagctggaaa cttgttattt gtatctggac
     1621 aagttcctct tgtaccagag actatggaag tagttgaagg agatgtacaa gctcaaactg
     1681 ctcaatcttt aaaaaatctt aaagctatat tagctgaaag tggagctgat ttttcaaatg
     1741 tagttaagac tacagtattt ataaaagata tgaatgaatt tggggctata aacgaagttt
     1801 atgctgaata ttttggagaa aataaaccag caagagcttg tgtagaagtt gctagattac
     1861 caaaagacgt taaagttgaa atagaagtaa tagctgtatt ataaaaatac aatattttta
     1921 atttgaaaag gctgaaaaac tatattctag tgattcagcc tttttataaa aaaataaatc
     1981 acaataaaga acatgataaa tattgtcgaa ttaaaaaata aaattaaaat aaatattgca
     2041 atatttttga gaaggtttta taatgagtat ataatttgaa acgtgtaact attaaattag
     2101 gcatgagttc ttggatattt ttatcctgag gattcatgcc ttttttgtat tgaaattcaa
     2161 aaatctattt aataaggagg aagtactgtg ttaaattttt tcaagaaaaa taaaagctat
     2221 aagttacatg cagtagtgag tggtaacagc attaatattg agaaagtaaa cgattctgtg
     2281 ttttcaaaaa agttaatggg agatggagtt gcaattattc ctaatagtaa tgttgttgta
     2341 gccccttgta atggtaaagt aactgtgctt acagaatcaa agcatgcctt tggtatggtc
     2401 tcagatgagg gtgttgaaat attagtccac ataggaattg atacagttag tttacagggt
     2461 gaggggttta aaaatgaagt gtctcaagga gatactgtta aaaaaggtag tccaataata
     2521 agttttgaaa gagaaaaaat aaattctcag ggaattgatt gtacaacaat aataattgta
     2581 ttaaatcata gcgaattttc agaaataaat tgcatggtag aaaatgaagt tgttgcaggg
     2641 caagatactg ttattgaaat tatgaaataa tagaaaggga ttaattcaat atgagagtag
     2701 taaaaatctt taataataat gcattatcta cagtaactaa tgataggaaa gaagctattt
     2761 tactaggtct tggtataggt tttaataaac gtccaggaga taaagttaat gaagataaaa
     2821 tagaaaagat ttattatgtt caagaccaca tgcaaacaaa atttttagaa ttattaaaaa
     2881 atgttagtcc agaagtgatg gatgcttcac aacaaattat aagtcttata ccaggaaatg
     2941 agggaaaatt taataataaa gggatattat ctttagtgga acatattagt tttgcaatag
     3001 atagaatgaa aaataatgtt tttttaccta atttaatgct tttggatata aagatgatgt
     3061 attcaaaaga gtttgaatta ggagtaaaag ctttagaaat tatatataga gtatgtcata
     3121 tccatttgcc tgaagatgaa gcaggttaca tagctttaca ttttgtcaat ttacaatcaa
     3181 atgataattt ggcatatgat actttaaaat ttgtaaaagg tagtattgat ttgattaaag
     3241 aatgttatgg attagaatta gacgaatcga gtttaagtac attacgtttt agaactcatc
     3301 ttaaatttct tgctcaaaga atttttaaga atgaaatttg tcaggatgat aaaatgattg
     3361 aaatgtatga ttatttaatt aacaatcatc ctaaaaataa agaatattta gaaaagttaa
     3421 atgtatatat tgaaaaggag tttaggtata aattagataa acctgaaaaa atttatctac
     3481 tactacacct aacaaaaatc ttataagaat gagatgaatt agtaatagaa atatatataa
     3541 ttttaggggg ggataagatt gaaagaaaaa ctacttggag gactggaaac atttgcaaag
     3601 gcaatggttc agccattaat gtatttatcg agtgcaggaa tattaatggt ggtaggtgta
     3661 ctacttacaa atgatatgtt attaggagta cttccatttt ttaaatggga gccaattcaa
     3721 atatttggaa gcctaattta taactgtata atggctatta ttaataatct tgctgttata
     3781 tttgctatag gtattcctgc tgcactagca aaaaaagaga agcacaaagc agcgttaata
     3841 ggatttatga gttatataat ataccttaca gcttccaaca ctatgttaac atcactaggt
     3901 caactagcaa aagaaagcaa gatgcttgga ctgataggga ctggacaagc tactatttta
     3961 ggtattcaag ttgtagatac aagtgtattt ggtggaatta ttttagggta tatagctgga
     4021 tatgtattta atcgtacaag caacaaaaca tttaaaggag ctttacagat atactcggga
     4081 gcaaatttta gttttatatg catgttcttt gtatccattt taattggagt tgtgagtaac
     4141 tttatatggc cattagctca acaaggaata gcttctcttg caaatgtcat caaagataca
     4201 ggtacatttg gtttattctt atatggtttt ttagagagaa tattagtacc aactggactt
     4261 catcatttaa tttatacacc atttcagttt tctgatttag gaggagtgct taaagttggt
     4321 gcagatacag tttcaggagc ctatgctata gttatgacag aaatgaattt accagttgag
     4381 cgtttctcag atagtatata ctacatggca actggattta caaagatgtt tggttatata
     4441 ggtataggag ctgcctttat ttatactgca tataagcaaa ataagaagaa ggttaaggca
     4501 acgattattc cactaataat tacggcagta ttagcctctg taacagaacc attagacttt
     4561 atgtttgtat tctgtgcacc acttttattc tttgtacatg cagttatagc tggtcttttt
     4621 atagctttat taaaaatatt tgatgttact gcattatgtg gagggaactt attagtatca
     4681 gttataatga atactacagt aggcgtagaa aaaacacact ggccagtaat gatgatatta
     4741 ggacttgcac aaattattgt atattttgta gtatttagtt ttttaattaa gaaatttaat
     4801 tataaaactc caggtcgtga agatgagagt acagctacag gcgaagaaca atcaaaacaa
     4861 cttaatttag atgcagggat agaaaatata attgatgggt taggcggaaa agaaaatatt
     4921 aatacagtag aaaattgcat cactagatta cgtgttaatg taaaagatga aagtaagatt
     4981 aatgaagata taattaattt aacgccaaat agcgggattg taaggaaagg aaaagatata
     5041 caaataattt ttggacttca tgtgcatgag gtaagacgtg cagtagaaga ttttttagaa
     5101 gcttattaaa gatatatatt tatatagcaa gggtaaataa cagctttaag tgctgtttac
     5161 tatttattaa agatacatat aatttataga ggaggaataa ttattatgat tattactatt
     5221 gttggtggag gaagcacatt tactccaggt attgttaagt caattgcatt aagagaaaaa
     5281 gagttagagc ttgaggaaat tcgtttattt gatattgata aaaaacgtca agataaagta
     5341 gctgtagttg tcaactggat actaaaagaa gatgttaaaa cagatgttaa actaactgta
     5401 actaattgtg aaaaagaagc gtatacagat gctacattta tatttgcaca aatgcgtgtt
     5461 ggtaaatatg aaatgcgtga gcaggatgaa aaaattccat tgaaatatgg ttgtgtaggt
     5521 caagaaactt gtggttgtgg agggatggca tacggactgc gtacaataca accaatgata
     5581 aaggttattg atgatgtaga gaaatatgct aaaaaagatt attggatatt aaattactct
     5641 aatcctgctg caatcgtttc tgaagcttgt cgtaagttaa gaccaaatgc aaggattata
     5701 aatatctgtg atatgccaat agctattata gatatgatag cggggtcatt aaatatcaat
     5761 gacgttcata atatacgcta tgattatttt ggtctaaatc attttggctg gtttacttca
     5821 atagattata aacatagaga cttaatggaa gagattaagg aatacataaa agaaaataaa
     5881 atattattac caaaatctta tatagaaaac ttaaataaaa gtcgtagtac aggaaatcgc
     5941 catgctgtaa gtagctggat acatgtttgg gaatctgttt atacaatgtt agaaagcttc
     6001 ccaaaatatc tgccaaacac ttatttaaca tactatcttt taagtaaaga aacagtggaa
     6061 cattcaaaca taaatcgtac tcgtgcaaat gaggttatgg aaagccgtga aaaagactta
     6121 tttgaaggtg tgagacatta cttagaaact ggagaaattt ctgaaaaagc attttatgca
     6181 ggttcacatg gagattggat tagtgatttg gcagtttcaa ttaaaaatga tacacgtagt
     6241 cgttttttag ttataactga aaatcgtgga gctataccaa acatgccata tgatgcaatg
     6301 gtagaaattc cagcatatat tggtaaagat ggtccagaag ttattgctcg aggtccaata
     6361 cctctatttc aacaaggatt aatgatgcaa caacttaaca gtgaaaaatt gatagtagaa
     6421 gcttatatag aaaatagtta tgaaaaagct ttacaagcat tcacattaaa taaaactatt
     6481 ccaagtatga atgttgctaa agaaattttg gatgaaatga tagaagttaa taaggaatac
     6541 tggccagaat taagatagta ggataaaaga aagggttttt atttaatcat tagatatgtt
     6601 ttcagagtct tttatcaaaa tgtattcaca aacatatggc aatatattaa aataaagagt
     6661 gatattttta gaagaatggg gtgatgcaat gaacattata aataaaattt tttggcaaga
     6721 agataggatt ggcatgatta caaataattt agaggcagat acacatagcc actgtatgtt
     6781 acaactgttc ttagggatag aagacagcat tgaaataaca gttaatgaaa aattagttaa
     6841 atgtaattgt atcatagtag ataaaaatat ctctcattca ttttcagcaa gaaaaaaagt
     6901 ttattactct gctattattg aacctacatc tatatatgca gaacagctta cctcaaaaat
     6961 gaatgatttt ggctactgga tatgtgacaa tgatggatta gagaaattac gtcaacaagg
     7021 aacttttttg atatctaact ctagtaaaga acaatattta agatttatgg aaatgcttaa
     7081 taactatttg aatataccta taactctcaa acattatgat gacagaatta ctgaattgtt
     7141 caatttatta catacttgta attgtgataa tcatactatt tcaagttttg cagataaagt
     7201 gtctctatca gcaagtagat tatcacatct gtttaaagaa caaataggga tacctttaaa
     7261 aagctatatc ttatttcatc aaatggagtg tgcttttaga gaattacttt ctggaaaaaa
     7321 tgttaccgaa gcttctatga ctgctggatt cgatactcca tcacactttg cagggacagt
     7381 taagcgaatg atgggaatgc ctgtttcttt atctttgaaa gatagcgagt ttttgaaagt
     7441 atattaaata ttctcgttat ataatgaagt tgtactaaga aaggggcttt gttatgaata
     7501 aagaatattt agaagaaaca aaaatgttaa attataatga accacaactt aagcttttgg
     7561 tatcatcaaa gaattggttg gaattagatg attttcataa aataaaatct atttatgagt
     7621 ttgtacagaa tgatatttta tttggatata atgcttctga tatgttaagc gcaacacagg
     7681 tattaaatga tggtatggga caatgcaata caaaagctac tcttttaatg gcattattac
     7741 gtgcagtgaa tatcccttgt aggcttcatg cttttgatgt aacaaaagat tttcaacgag
     7801 gagctacatc caaattgatt tcactattag caccaaaata tattcttcat acttgggtag
     7861 aggtatttta tcaagatagg tggattgctc tggagggtgt cataactgat aaaaaatatt
     7921 tagaagcaat tcagaagaaa ttttttaatc atggtggaac gtttaaaaag tatgcaatag
     7981 ctacaaatga tttgaaaaat acatctattg attgggatgg taaagatact tttatccaaa
     8041 aagaagctat cgtgtatgat tatggtattt ttccatcacc agacgtattt ttttctactc
     8101 attcacaaca tatgtcaaaa ttaaaaaatt tcatatatgt acatcttatc agaaaaataa
     8161 tgacaaaaaa tgtatgtaag gctagaaaca attatattga taaaaatgaa tgagtgttta
     8221 agaggtaact atataggaaa tatatagaat aattaaaact gtactggtta aaatgaataa
     8281 aaaggagtta tcacagagca aacttaaatt gttcatagga taactccttt tctgtattaa
     8341 cttctaataa tttgatgtct ttttataaaa catatagata caattaaacc agtaaatgca
     8401 agagtagctg ctattgaaaa tgcaacatct attccatgaa tgttagacag catgtttgaa
     8461 ctcatatgag ctttcttagt agcacttgtc attatagtaa taagtatagc agaacctata
     8521 gctccagata tctgacggag tgtgttatta attgctgtgg catgagatat aagttctcta
     8581 tctaatgttt taattcccca tgtagttaat ggcattaaaa ccattgaaat accaatcata
     8641 cgaatacaat acatcaatga cacatgaatt aaagatgtat ctctgcctaa aaaagcgaag
     8701 gataaagttc ctaaaaacaa acatcctgtt cctaatattg atagagcatg aggtccatat
     8761 ttatctaaat gacgtcctgc aattggattg agtacaacca tcaatatagc accaggtaac
     8821 attaatgaac ctgaagataa tgcagaaaat cctcttacag attgaacgta cagaggaatc
     8881 attaatgtag cagacatcat tgaggcatat acaatcataa ttaatattgt agaaataaga
     8941 aatggtttat ttttaaaaac cctcaattct aaaaaaggtt ctttagattt taattgacgc
     9001 aatgtaaata aaattaatga cataattcct attaatattg gtaaatatgt ggcaatatta
     9061 gtccacccat aattaccttg attagtaaat ccaattaata aaccaccaaa acctagagat
     9121 gatagtacta ttgaaggtat atctaatttt agtttttgag tttccccaac atttcttaat
     9181 aaaataaatg atagtataac atctaatata gcaatagggg aaagaaacag aaataaacta
     9241 tgccaaccaa atgaatctac taaccatcct gataaagttg gacctattgc tggagcaaat
     9301 cctactgtaa taccaactaa actcatagct gcaccacgtt tttcctcagg gcataaatag
     9361 agtataataa cttgtaaaag tggcattaga gaaccagaac ccattgcctg caacacacga
     9421 gcaatcaaca tagttgaaaa attattagaa aaaagtgcaa taatacatcc tatagaaaag
     9481 atagacattg atgtaataaa taactttcta gtactaaatc gatttattaa ataagctgtt
     9541 gtgggtacaa taatacctgc aataagtaga taaattgttg ttaaccactg accaacatca
     9601 gcagtaattt taaaatctga cattatatta ggtaatgctg ttgacaatag tgtttgactg
     9661 agagaactta taaaagcacc agtcattaca attgctatta ttaaatttcc ttttttattg
     9721 ttactttggg tttccataat ttataacgtc tcctttttta attgattttt aatgctgttc
     9781 aataaaagta cttttggtct taaagtgtct tctttttcta aatatgcaat cattgcatcc
     9841 ataattaaag aatcatattg ttgatttttt ccctcgacgt ataatttttt tattgcttct
     9901 agtaatttag gattttcaag gagagaagta tcagttttga ttagttcttc tatatcttgt
     9961 atcattttaa aaaatagcga tttattatga agggaaaagt aaacttcatc ttcttgttta
    10021 tgtaaaaaca ttgatttttc aattaaagga tttagattat ccatattttc tacaactaaa
    10081 tctagaattt gtatagtaaa tttggcaata aagtatacat ttatttctct tttttgtaca
    10141 tgaataatgt atataaactg atgaattaga gcatccaata agaaaactaa atcaaatata
    10201 attttttcta ttttatcacc atagttatca aataagctta atttgtattc attcaataag
    10261 ttagctcgtg attttgtttg caatttactt gtagccttac gttgctcttc agaaaaattt
    10321 tctcttatat aggtatttat ggtagttcta gaaaaaatat ttttccatac agcaattatc
    10381 tttctgttta acttttctga tgctggaatg tcattatttt catttatgga atgtataatt
    10441 tctaaaaact tttcatcata ataattgatt atttcgcaga taaaactttc cttagtttca
    10501 aaatgcttgt aaaaagtagc tttagacatt ttacactctt ttacaacatc cgatattgaa
    10561 gtattttgaa aacccttttg gtcaaaaatt ttttgtgcaa tagttataat gagcctttgt
    10621 ttttctgtca ctttattatc tcctttcacg caaaaacgtt tagtactata gagtacctac
    10681 aagttttaat aaatatatta tacatcattt ggaaaaaaaa tcaatatata gactgtaata
    10741 tgaaattttt gaaaaatttg ttgttatttt tagtcgtata aaaaagaaac ttaagagttt
    10801 cttaagaatt gtttaaagaa gttgcaataa attagtggta tgattatagt atgaaagaga
    10861 atgtcttgaa atttttctgt atcaaattcc aattatggag gtgtagtata tgaaaaagaa
    10921 aatttgtgta gcaataattt ttgctctatt tatagttttt gtagaaatgt ttattgatag
    10981 ctctagcgaa ggaagtaaaa ttatgacagc tgaattaagt aaatacagga aaggcgtaaa
    11041 tgtattatca ataaacaata ttatagtagc gaataaaaaa tatagtttgc ccaaagatta
    11101 ttctcctcag gaaagcagtg aagcgagaga tgctttttat aaaatgaata aagatgctca
    11161 aaagtcagga ttaaatttaa aagcttttag tacatataga agttatgaat atcaagatag
    11221 attatttaag tcatatgtaa aagaacatgg agaaaaagaa gcaaatagat tttcagctaa
    11281 acctggagag agtgagcatc aaactggact tgcttttgat attggaggag atgaccagtc
    11341 ttgttgggca aataaaaagt tcaacaatac aaaagaagct aagtggttgt atgaaaatgc
    11401 atataaatat ggttttatac ttagatatcc agaaggtaaa gagaatataa caggatatat
    11461 gtatgaatct tggcactata gatatgttgg aactgaacat agcaaaaact ttgcaatgaa
    11521 taatttgact ttagaagaat atttacatat aaattaagag atggtttgtt aaaattaggt
    11581 taaaataaaa aataacctag actattagct gtgtaaagtg atataatgta aatacaattt
    11641 agataactac tcgaagggga gtagcttaca cgaaagtggt aattaatcgt caagacagta
    11701 gaaatactcg gttaattagc aagggattcc ttgatagcaa gaccttcaaa ataagcatta
    11761 ggtatgtttg ttttggggtc tttttttatt caaggaattt ttatttaaaa aaatttaaat
    11821 ttcaagtata cataagagcc cccaaacata aaataaattt attgaagagg agggcaacaa
    11881 atgtcttaca aaagtactta tcaagaagta attaaaaatt tagactcaag tttatctggt
    11941 ttgagtaaag aaagagcaga agagttgtta gaaaaaaatg gagctaatga attaaaagaa
    12001 gcagataagg ttcctactta taagcttttc ctagaaagtt ttaaagaccc attagtaata
    12061 attctattga tagctgcttt agtacaaata tttttaggtg aaacagtgga gtctataata
    12121 atttttgcag ttattattat aaactctgtt ctgggtgttg tacaaacaaa aaaagcagaa
    12181 agttcattag aaagtttgaa aaatttatct gcaccaaacg caaaggtaat aagagacaat
    12241 aaaaaaatga caattccagc aaaaaatcta gtagttggag atattgtatt cttagaagct
    12301 ggcgattaca taccagcaga tggaaggctt atagaagcac aatctctcaa ggttgtagaa
    12361 gggatgttaa ctggagaatc agaaccagtt ttaaagcata cagaaaaaat aagtgaagat
    12421 gtaagtttag gtgaccagaa aaatatggta tttagtggtg ctgtagttgt ttatggtaga
    12481 ggttcttttg tagtgactgg aacaggtatg gatactgaaa tgggtaaaat agctgggctt
    12541 ttagaaagtg ctgaaaataa acttacacca ttgcaacaaa aaatagagga ttttagtaaa
    12601 aaacttggtg taggtattgt aatacttgct cttttaatat ttataattca agtagcaaga
    12661 aattacttta tggctggaaa tggagaaatg acacaaatta ttttagattc attcatgttc
    12721 tctgtagcag tagcagtagc agctatacca gaagctctat catcaatagt cactatagta
    12781 ttggctgttg gaacaaatag tatggcaaaa aaacaagcca taataagaaa attaccagca
    12841 gtagaaactt taggttcaac aagtgtaata tgtactgata aaacaggtac tcttacacaa
    12901 aataaaatga cagtagttga ttattatatg tatggaacta ataatgatga gttagataaa
    12961 gaaaaagtaa actattctta tcatgctaaa ttattgacta tggtttctac attgtgtaat
    13021 gactctcata taactagtga tggtaaagaa gttggtgacc caacagaagt agctttaata
    13081 aattattcaa gtaaaaatga tttagattat gataaattaa gaagtaagta tatcagaata
    13141 aatgaaatac catttgactc agacagaaag cttatgtcta ctgtaaatag tatatatgga
    13201 gattatataa tgtttacaaa aggtgctcca gatattgtgt ttagtagatg taaatatgct
    13261 ttgaaaaatg gttcaaaagt agatataaca gatgagataa tagaagaata taaacataaa
    13321 aatgaagagt tttcaaatag agcacttaga gtattagctt ttgcaataaa agatgtccca
    13381 gaagaagatt ttataccttc aattgatgac gaacatgaca tgacattggt tggaataatg
    13441 gcaatgatag acccaccaag agaagaagta atggatgcag taaaagaggc aaagagtgct
    13501 ggtataaaaa ctgttatgat aactggtgac cataaaacaa cagcagcagc aattgcaaaa
    13561 gagattggta taatggaaga aggagacttg gctcttactg gaaaagagct tgatgctttg
    13621 agtgaagaac aactatatga aaaactagaa aatataagtg tatatgccag agtctcgcca
    13681 gaaaataaaa ttcgtatagt aaaagcatgg caacataaga ataatataac agcaatgact
    13741 ggtgatggtg ttaatgatgc acctgcatta aaacaagcag atataggaat cggtatggga
    13801 agtggtactg atgtagcaaa agatgcttct gctatggtat tggttgatga taactttgca
    13861 agtatagtaa atgctgttga agttggtaga acagtataca gtaatataat aaaatcaatt
    13921 acttatctat ttgcaggaaa cttaggagct atagtatcca tagtatttgc agtgtttgca
    13981 ggttggtcaa atccatttac agctttacaa cttttgttta taaacttagt aaatgactct
    14041 ttaccagcaa ttgcattagg gtttgaaaag tcagaaaaaa atataatgaa taaacctcct
    14101 agacacccgg atgaaagtat actagcagga ggaacattga aggcagttgc tataagaggt
    14161 tgtataatag gtgtagttac aataattgca caatatatag gattacaagt atctccagaa
    14221 cttggagtag caatggcatt ttcaacattg atattagcta gaataatgca aacattacct
    14281 gcacgttcta ataatcaaac agtaataaaa ttaggttttt tcagcaataa atacgttata
    14341 ggagcagttt tagtctgtct tgggctttac tctttaacac taataccagt gctaagagga
    14401 atattctcta taccaacaac gtttggattg taccagttgg ggatttgcct agggttagca
    14461 ttaatttcat caatattaat ggaacttaac aaattaataa atagaagtga agtaaaataa
    14521 tcaaaaaatt aaagggcata ttgattttaa caatatgcct tttataaata gtctaattta
    14581 ttctatatta tcgattattt ggaatgcttt tataacttca ggtaattctt tttcttcagg
    14641 cattctaaat cttatgaaat ttttatcaag tccagtaaaa tcacttccag atataactct
    14701 tatttttaat ttagcaaact cttgttgtaa atctatgtcc ttgtttttat gttcaactgt
    14761 cataattgat acagtatcac tagtttcagc tatattaagg tttttccatg gaattaataa
    14821 ttgatttttt atatcttttg ttttttcttt taattcttct ataaattgaa catcatctaa
    14881 taaatttgct gcaatactac gagatatttc agacacttcg taaggagtag aaatcttttt
    14941 aatagggcta acaagttgtt ctggaagaac tgcatatcct gctcttagtc ctgctaaacc
    15001 gaatcctttt gaaaatgttt ttaaagcaat tacattatca taattattta gaagcttgac
    15061 agctgaattt tcttttggca tgtattcacc ataagcttca tctaccataa ctgctatatc
    15121 atatttagct gcttccttta ctatattttc aattgatgat aaatgtataa tttgtcctgt
    15181 aggattgttt ggattgtcta tgtaaattac tttatattct ggatttattt tttcaataaa
    15241 ttctttttca ttaaacttga aattatcctc tttctttaaa agaacataat cataagttgc
    15301 accatgcatt tttatatctg tctcatattc tgagaactga ggtgaatatc ctaaaacttt
    15361 atctcccttt tcaataaata gtctatttaa aaggtaaata acatgtatag aaccatctgc
    15421 caaacaaatt ctgtcagtat ctaatgctat aaaatctttc caataatcga ctatattatc
    15481 ttttaaagca attgaatgag gatatcctct tatcatatca aattttaaag cattaaaagc
    15541 ttctacagct ttgtttgagt aagacacggt atttatacct tcaccacagt ctatctcgaa
    15601 tacatcttct gaatgtccag attctattgc ataacttttt ttcaactgca tttcaatagc
    15661 tttgttaatt tttagtttca tactttatca ctccttattt agtcaataat aaatgttata
    15721 aaagttgcta gatttaaaat attatgaaaa tatataatag taaaaaatga atataaatat
    15781 tataatagct cactttattt aatatgatta tatatcatat attgtaaaat ttctagcatt
    15841 ataagactat aaattgaaaa tattcagaaa aatactacaa gaaacgattt attttgctct
    15901 taaaataagt taaatgaatg ttattaattt tgtaaattga aatagtttaa gtataaagtt
    15961 atgatataat ctaataaggg taattcttta gattatatta aggaaatata ggtatacaat
    16021 aaaaagttat ggagttggat tatgggatat aaattaattg taacagatat ggatggaact
    16081 ttattaggca ataatcataa agttacagat gaaaataaaa ctgcactaca aaaagttata
    16141 aaaagcggta ttaatgtaac tttagcaaca ggaagagcct tcgattcagc taaatgcaat
    16201 gtagattttc ttaaagagga catgccaata atagcttgta atggttcatt aataagagag
    16261 caaaatggaa atataatata ttcaaataag attgatacaa gaacatgttt aaacatttta
    16321 gatgtattag ataaatacga tatttactac caatgtaata gtatagattc tatgctttcg
    16381 aaaaaaatag aaggacgtga agatagatta agtgtatttt taggctctga aacagaagta
    16441 attgtaaaag atgatttgag agaagaaatc tttaaaaaag atatactaaa atttgtaatc
    16501 atagaagaaa aaaatccatc tattttagat gaaataagaa aagaattgag aaaagttcaa
    16561 ggaataaaga taacttcttc atggccaaac aatattgaag tcatgaatga aggtgttgat
    16621 aaaggtaatg cagttaaaat tttagctgaa aaaatgaata tagacagaga agatataatt
    16681 gcatttggag acaattacaa tgacatagaa atgataaagt ttgctggtct tggggtagct
    16741 atgggaaatg cagaagaatt gattaaacaa gaagctgatt atgttacaga tacaaatcaa
    16801 gacagtggag ttgcaaaggc aatttataaa ttcctagagt taaaagaatc acttagctct
    16861 taaaacagta tataatagga aaaaataggt tgactatttt gtagatagta attataatac
    16921 aaatagataa aacataaaaa tttagattca gtgaaaaaga gagtagatat agagaaaatc
    16981 tcagcgaatt gggggatggt gtgagcctaa tgataacact ttatattgaa aagaactttg
    17041 gagaatacta cttgaaattc agtatggtag taggtaatct cgccttaaga gaatagagtg
    17101 ggtaaacatt gtttaccaac tagagtggta acacggaata tttcgtctct tggtcttttt
    17161 gaccaagaga ttttttagtt tcattgagtt acaagactta taattaaaat acaaaaaatt
    17221 aaaatatgag gtgaaagtta tgaaattatg gggcggacgc tttagaaaag cggaaaatca
    17281 acttatggaa gaatttaata aatcttttgg ctatgattgt gtactgtaca aaaaagatat
    17341 cgaaggaagt gtagcacatg ttcatatgca agtaaaatgt ggacttttaa cagaagaaga
    17401 aggaaagtct ataacagaag gtttaaaagg gatacttgaa gatgtagaaa atggaaagtt
    17461 agtattggat ggagagtatg aagatataca tagttttact gagataaatt taattcaaag
    17521 aattggagat gttggcaaga agcttcatac agcacgtagt agaaatgacc aagttgcagt
    17581 tgatatgaga ttgtatgcta aagaaaaagc aaatgatttg gtgggtttaa tatctgaatt
    17641 taaagctact ataaaagatg ttgctgacaa aaacccagtg atgatgccag gctatactca
    17701 tcttcaaaga gctcaagtag tgacatttaa gcatcactta atggcatatt atagtatgtt
    17761 tgatagagat gagaaaagaa taaaaaatgc tatagaaata ttagatgaat cacctttagg
    17821 ctgtggagct ttagcaggaa ctacgcatga tatagataga agtataactt gtgaacaatt
    17881 aggatttaaa aaagtagtag ataattttat ggatggagta agtgatagag attatttact
    17941 agagctaatg agtgattttt caattataat gatgcactta agtagattaa gtgaagagtt
    18001 gatactttgg agttctcaag aatttggatt tgttgagata gatgatttat atacaactgg
    18061 aagtagtata atgcctcaga agaaaaatcc agatggtgca gagcttataa gaggtaagac
    18121 tggcagagta tatggaaact tatttggtct ttttacagta atgaaaggaa taccattagc
    18181 atataataaa gacatgcaag aagataaaga aggttttttc gatagtgtac atactcttga
    18241 aatgtgtata caaataatgg atagaatgat agccacacta aaggtaaatg aggataaaat
    18301 gaaacaagca gtaaaaaatg gttttttaaa tgcaacagaa gttgcggatt atttggttaa
    18361 aaataatgtt gcttttagag atgcacatgg tatagtagga agtatcgtta tatattgtga
    18421 agataataaa aaagcaatag aagatttaac attagaagaa ttacataagt tttcagatgc
    18481 ttttaaggaa gatatttatg attttatcga ctatgagagt atattaaata agggaataaa
    18541 aaagaattta aaataatgta tttattttta aaagctgtaa atttgaatgt tatcatttaa
    18601 atttacagct ttttattgtg taagatttta ttatataaaa gtaagaaact taaaagtagt
    18661 agtatttaaa ataaatacaa ataaaaaatt gttattaaaa tgataattaa tatcaataag
    18721 tattgacaaa attatttcta aatactatta tatataatat aaataataat gaaaatcaat
    18781 atcaaataaa tgggaggaaa tctaatgaac ttattagtga ttggtggtca tgaaagaatg
    18841 gaaaaagatt atatggttat ggccaagaaa aaaggttata agacaaaggt atatactacc
    18901 atgtcatcta aattaaataa ttctatagga agaccagatg cagttgtcat acttacatca
    18961 actgtttcac ataagatgtc tagaacggtt gagtctcaag ctaaaaaaca agatatatta
    19021 atcgtaaggc ataaaaatag tagtaaggta gcatttaatg aatgtctaga tatggttgat
    19081 gaatgtctgg gaaattgttc aaaatgtaag cttaaagaca aacaaaaaca aaaaaatata
    19141 taggtataaa aaatacataa acataaaatt aaaagtactg agataatagt tataattatt
    19201 atctcagtac ttttagattt ataaggataa gtatattata aattttttat tataatttcc
    19261 aactacttgt tactttttca tatttttttt acttctagct ttttttcttt ttttcttctt
    19321 tatatttgaa gctattataa ataaaactaa aagcactaga atacctgcaa taatcttaac
    19381 aaaagtcatt aaaatattat ttccagtaaa aaatggaaga ctgctatcaa gattattttt
    19441 tgcaattaag tctatactgt tttctagttt accattatta tatacattta aagtaccaac
    19501 cttagcccct ttttttatag gcaaatcaat tttatttaac tctgttttgg tatcataatt
    19561 gccttgagat tcatcttttt ctaagaatat cctataacta ttttttggtt cataaatcaa
    19621 ttcatgttgt tttgtaaaca atactttttt agtatctgtg tatttttctt tatcaacaat
    19681 agtagcacac ttaaagttat caaatccata atcaatcaga atacgtgagt ctaaatataa
    19741 atcagcatta gttgaattaa atacagcaac gataactctt ctgccatctt ttacagcact
    19801 cgataaaaga catttaccag cagcatcagt atatccagtt tttataccat caactatatc
    19861 atattttata tttataggtt gacctttgta tgtaatctta tcatgtgatg tcaaaaactt
    19921 attagtgttt acaaagtatc tctcataagg atatgctttt gtagcttcaa atttaagtga
    19981 cttagttttt acaatttgtc taaaagtatc attattcata gcttctctag ctatgagagc
    20041 catatcatga gctgttgtgt agtgatttgg gtctggtaat ccatgaggat tattaaaatg
    20101 agtattagta gctccaattt tttttgcctc agagttcata aggtcaataa atttttgtac
    20161 attgccacca ccaacatatc tagctaatac aaaagcagca tcattagcag aatgaaccaa
    20221 aagagcatct agtaactgct ttacagtaaa agattcccca ttttttagat acatactaga
    20281 gccatctatt tgaggtaaat ctttaatttc tattacttga tttaaatctt taacctcctt
    20341 tagaactaaa caagctgtcc atactttagt tgttgaagct ggataaagct tagaatttcc
    20401 atccttatta tatagtattt tgccagactc atagtccatt aaaactgcat gtttagcaac
    20461 tatagctggt tcattatctg caaaagagat attagagctt cctaaaaatg aagtaaatat
    20521 caataaacag attaataaaa gtgataaatt tcttttcatt gtgagcctcc aaattttaaa
    20581 ttcatctttt agtatagcat atatttgtca ataattaaaa gaaaggagtg attaatatat
    20641 gaaaaaaaaa gtaaaattta tttcgttttt tgttttattt attttaataa tatcatcaat
    20701 aatcgtttat aaatcgctta ttttcaaaga tgatatttat gttgtaagta aaaaaaattt
    20761 acctaatgaa aaaacacatg ttgtagagaa tgaaattaaa ggtgaagata ataatcacaa
    20821 taaagtaact aaagacatag ataaaaattt tcaaaataat aaaataacta tatacataag
    20881 tggagcagta aatagacctg gaattgtaac tatagagtca gacaagagac tttatgatgc
    20941 tgtagaatta ctaggaggaa cgacaaaaga agcagattta aatggagtca atctatcagt
    21001 aagattagaa gatgaacaac attacataat acccaaaata ggtgaagcaa catctgttac
    21061 aagtaatgat gattccaata aaccaaatca gaggaatgag tcaagaggtg agcctaaaaa
    21121 taaatccaat aaaaatatat ctaacgaatc aaaagtaaat ataaatgtag cgactataga
    21181 agagttggat tctctacctg gggttggaga agctacagcc aataaaattt tacaacacag
    21241 agaagaaaat ggacagttca gttcaattga ggaaataaaa aatgtaaatg gtattggaga
    21301 taaaaagtat gaaaatataa aagatttaat ttgtgtagac taaaatataa tacaaaactt
    21361 acatatagat atgttattat aaatatgtat gtatatgtgt gtacatgtgg aaatttagta
    21421 aaggaggcac ttttatgaaa aaagaacaaa aaaaacttac agagatgaca acagcaggtg
    21481 gttgagccgc taaaataggg ccagaggttc tggcatcagt tttatctcaa ttgcctaaaa
    21541 atgataatat agaaaattta ttagtaggac tagatacagc agatgatgct gcagtttata
    21601 aattaaatga tgatatggca cttatccaaa ctttagactt ctttacacca atggtagatg
    21661 acccatacgt atttggtcaa attgctgcat caaattcact atcagatgta tatgctatgg
    21721 gaggcaaacc tttggttgct atgaatattg tttgttttcc atcatgccat gatatggatg
    21781 tacttgcaga aatattaaaa ggtgggtttg ataaagtgaa agaaagtgga gcacttttgg
    21841 ttggagggca tactgtagat gacaaagagc ctaaatatgg tctttcagtt tctgggattg
    21901 taagtccaaa taaagtttta tcaaatgcta ctgcaaaacc tggtgataag ttaattataa
    21961 caaaaccaat tggagtgggt gtattaaata cagccatgaa agaaggtatg gtagaacaac
    22021 atatagcaga taaagtaata gaaataatga tacatttaaa taaatatgca gcaatgagtt
    22081 ttgagaaatt tgatgtaaat tcggttacag atataactgg ttttggtcta cttggtcata
    22141 ctttagaaat ggccaaagct tctgaagtaa gtattgaaat tgaatcaaaa catgtaccaa
    22201 ttattgaagg tgcaatagaa atggcacaaa tgggaattat acctgctggt atgtataaaa
    22261 atatgcatta tgtatctaaa gatgtagaag ttgtaggaaa tatagaagtt gcagttcaag
    22321 atattttata cgacccacaa acatcaggag ggttgttaat ttcagtaaaa gaagaacttg
    22381 cagaggaact tgtaaaagat atgaaattaa atggagcaat agaagctaaa attataggtt
    22441 ctgtagttcc aaaaggggaa aaatatatta cagttttata aaatattata gagaaagagt
    22501 gattgttttg agcaaaagag aattgtttgc aatgttgcct tcagttgatg aagtattaag
    22561 tgataataga attgtagaaa taataaatga atatcctaga agtttagttt tagaatctgt
    22621 gagagaagta atagatttaa aaagacaatt tatattaaga ttaaaagaag atgcatcaaa
    22681 ctctgtaaca atagagtttg gtgaaataat agaatctgca atagagagag ttaagttaaa
    22741 ttactcatta tctttaaaaa aagttataaa tgcaactggt acagttattc atacaaattt
    22801 aggaaggtct ttacttagtg aagatataaa agatgaatta tggtgtgctg cttcaaggta
    22861 ttcaaactta gaatacgatt tggataatgg agaaagaggt tcaagatact ctcatttaac
    22921 tagtacaata aaaagactta ctggagcaga agatgtacta gttgtaaata ataatgcagc
    22981 ggcagtactt cttgtactta gcacaatggc taaaggagga gaagcaattg tatctcgtgg
    23041 agaattagtt gaagtaggtg gttcatttag gattccaagt ataatggctc ttagtggtgc
    23101 agaattagtt gaagttggtt caactaataa aactcatttg aaagattaca aggaggccat
    23161 tacagaagat acaaatgtat tgatgaaggt tcatacaagt aattatagaa taatgggatt
    23221 cacagagagt gtttctatag aagaattggt taatttaggt aaaaaatata aactacctgt
    23281 aatagaagac ttaggaagtg gagtttttat agatttatca aaatatggtt tatcttatga
    23341 accaactgta ttggattcta ttcgacaagg tgcagatgta gtaactttta gtggagataa
    23401 gatgcttgga ggtccgcaag caggtattat agttgggaaa aaagagtata tagaaaaaat
    23461 gaagaaaaat caacttacta gagcacttag agttgataag cttacaatat gtgctcttga
    23521 agcaacattg agaatgtatt tagatgagac aaaagctata gaaaatatac ctactttaaa
    23581 aatgttgaca tataaaattg aagaattaga agtaaaagca aataaactat ttgaaaaaat
    23641 aactgcttta aacttaaatg caaacataaa tatagaagat ggattttcac aggtgggagg
    23701 agggtctatg cctcttgaaa ctataagtac aaaggtaata tcaataactc ctgaacatat
    23761 gaatgtttca tctttagaaa aaaaactaag acttagtgaa gcacatataa tagccagagt
    23821 gtatgataat aaatatgtac tagatgtaag aactatattt gatgatgaat ttgatgtaat
    23881 agttgaggaa ttgagaaaag catttaatta aataaatttg agttaataaa tattaaattt
    23941 agaagtataa gtatgaagtt ttgattaaaa aatagctaca cacacttgtg actttagtca
    24001 tagcaggttt agagggggca aaaaatgaaa aatgtagtaa ttggaacagc aggtcatatt
    24061 gaccacggaa aaactactct tataaaagca ttgactggaa gagagactga tacgttagat
    24121 gaagaaaaaa agagaggtat ttcaataaac ttaggattta cttattttga tttaccaaac
    24181 aatagaagag ctggaattgt tgatgtccca ggacacgaaa aatttataaa aaacatgtta
    24241 gctggagcat caggaattga tatagtttta cttgttgtag cagcagatga aggggttatg
    24301 ccacagacag ttgagcattt agatattcta agctttttaa atatcaaaaa tggaatagtg
    24361 gttctaacta agtctgatac tgtagatgat gaatttagag agctagtaaa agaagatatt
    24421 tgtgaaaaaa taaaaggaac ttttcttgaa gatgctgaaa taattgaagt tgattctata
    24481 tctaaaagag gtatagacac tcttattgaa aaaatagata aaatgagtga tgatatagaa
    24541 gataaaaatg aaaactctcc agcaagactt aatatagaca gagtattttc aataaaaggg
    24601 tttggtactg taataacagg cactttaata gaaggaaaga taagtataga agatgatttg
    24661 gttatatatc cacaggtatt aaaaacaaaa ataagaagta tacaagttca tggagaaaat
    24721 aaagatacag cttatgcagg tcaaagaaca gcaatcaata tatcaaatgt aaaagtggat
    24781 gatataaaaa gaggagatgt tttagctgca ccaaactcat tggaggaatc aatgatgtta
    24841 gatgtaaaat tatcattggt aaatcattta gataaaggat taaagcactg ggatagatta
    24901 agattgtatc atggaactag agagatactt tgtagagcag ttccactaga taaagaatta
    24961 attgaaagtg gagaaagtgg ctatgttcag ttgagacttg aagaaagcat agtatctaag
    25021 aaaggtgata cgtttgtagt gagacgttat tctccaatgg aaactatagg tggaggtgta
    25081 ataattgacc catctcctaa aaaacataag aaatttgatg aaaaagttat agaagcatta
    25141 aaaataaaag aaaaaggcga actaaaagat ataatagaag agtacttaaa aaggaattta
    25201 aaaaattatc caaatataaa agaaataatg agctatagtg gtgctcatga agaagatgta
    25261 aaaagagcgc ttgaaacact tatatcagaa gataaagtat ttattatagg aaatatgtat
    25321 atgcatataa atcaatacaa taaattaaaa gaaaatacaa taaagctttt aagtgagtac
    25381 cataagaagt ataggcttag aaaagggata ctaaaagaag aagtaaggtc taagatagag
    25441 agtaatttta agactaggga gatggatatt ttattagaaa aattgtctac agaaaatgct
    25501 ataaaaatag aaaataatat agtatctcta ttagattttg aggttatttt aaatgataag
    25561 caaaaagaaa tagcaaaaaa aatagaaaaa agattaaaat catgtggagt ttcttctata
    25621 ctgacaatag atgaagtttc tgaaggaaat cataactatg ctgaagtatt ggagtcaatg
    25681 ataggtaata aagtagagaa attagatgat ttgtatataa tggacaaaga tatttatgaa
    25741 aatgcaaaga acattttgat aaattatata aaagaaaata aagaaataac attaggagaa
    25801 tacagagatt tgattgattc tagcagaaag aattgcatga ttatattgga aaacttcgat
    25861 agaaacaaaa taactaaaag agtagaaaac aaaagaattt tgttttagca gaaaatgtct
    25921 gtttttgctt taaggtataa agaaggaatt ataatgtcga gtatcgtata attaattagt
    25981 ataaaatatt aattaagagg aagtgtaaag tattgaagaa tataatacct gatgtaaaac
    26041 cagatataaa agaaagagaa gacatgagcc ttttatttat aataatgggc tcagtacttt
    26101 tattatttat tataaaagat atagagaaat atgaaacttt attacatgcc atatatacga
    26161 tatttttagt agctacagga gtaattatat ttaatactac aaaatttaaa gtagattcat
    26221 tttctacatt tttaggatta atttttatag ctactggagt attggagtgt atatatttat
    26281 ttaattcctt ggggattaaa actaagtcta taatggagat taacatcaca atatcagcaa
    26341 taacagattt atttcctata ctgggtgtat acttaagttt taaatttgtt aaggataaca
    26401 aacaaattta ttcaagtgta attttattta tagcaactgc tacattaaca ataacatctt
    26461 tattcataat atcaggaatt tgtgactatc tgggaaatgg taaatcacaa tatgctttag
    26521 gagttataat atctatattc ataataatta tttctatcat tagtggaatt gaattaaatt
    26581 caagttcaaa taaaggtaaa tgggaatatg atgaaaaaaa gatttttaac agaataatag
    26641 taataataat cttatcaaga gttccaaatc tattgcatat agttattgat aatagaaatg
    26701 tagagaggat attatcacaa ataataataa atgtagcctt atattattta tataattaca
    26761 tagtatctaa gaatattaag aaaacagcac tggaattgca tgatacgaat gaagaattaa
    26821 caaaaaaaac ggaaagttta aaagaaaaga ataaaaaatt aatctatgag acagaaaaga
    26881 tagaggaact taagggaata cttatgaaaa gagaatctag gctacaatct acattagatg
    26941 tagcagtaaa tagcatagta gtatttagca aagatggaga aattacatat gcaaataaga
    27001 gctttagaaa tacttttgga gactataaag aacaagataa attgactgat aaaataaaaa
    27061 acttcaatga tttgatagaa agcattcatt atgtgtttgt tcatgagaag aatgcagaga
    27121 aacttattag tacaagcgat aataaagttt atcaagctat atttgcacct ttaattattg
    27181 cttcacaaac agaaggagta ttgtgtgtac tagaagataa gactaagaaa aaggagtacg
    27241 aacgcaaact tattgaagca aataagagaa gtaaagattt tttagaaagt attggagatg
    27301 gcatagtagt gcttgaaggt aataacaaaa tatatgttaa taatgcatgt agggaaattt
    27361 ttaaaaataa tttagagagt attgattttt cattagtatg taaaagtgaa gaaagtatag
    27421 aaaaaagata tgtaatagat ggtgaagtaa agtatgtaga gatgagtttt tctcagtata
    27481 ctaatgctgg tactaataaa actattattg taatacgtga tacaacctct cgaaaaatat
    27541 ctcaaataaa acttgaggaa agtcaatcat catattcaag atttatagat atattacctg
    27601 atggcatatg cttattaaaa gaagatttaa gtataaacta tgcaaataag tcgtttttag
    27661 atatgctagc ttttacaaac atagatgata taaaagattc taatattaaa ctattaatga
    27721 atgcgaatac tgaagaaaaa atgaaattta cggataatat ggctaaagtt ttaaatgaaa
    27781 atagaagtat acttttactg gaatatgaat tgataaatag ttatgatgat atagtagaag
    27841 tagaattaag tgcattacct tttgctatat acaatactag atacataatg cttattataa
    27901 aagatttagt acataagaaa tattccgagc aagcagaaaa agaactgctt gaaagattta
    27961 aaacagataa aatcaaaacg gagttttttg caaatatgtc tcatgaacta aaaactccac
    28021 taaatgttat atctagttca aatcaactgg tagattcttt ctatagaaat gaaaagataa
    28081 aagattacaa taacaatatt aaatctcatg tggatttagt tagacaaagt tcttataggc
    28141 ttcaaagatt aataaataac attatagatt taacaaagat ggagtctggg ttctatacac
    28201 taaaacttgc gaaatacaat attgtatctg taatagaaga cttatttatg aatatagaag
    28261 agtatgcact tagaaaagac ataaaaattc tttttgatac agaccttgaa gaaattaatg
    28321 tttatataga caaggttgaa attgagagaa ttatgttgaa tctattgtca aactgtatta
    28381 agtttactga taatggaggc tggatatatg taagtattca ttataaaata gataaagtaa
    28441 tcataagtgt aaaagacact ggagttggaa taccacaaga taaattagag ttgatttttg
    28501 aagaattttc acaagttgat aagactttat ccagaaatac tgaaggaagt ggaatagggc
    28561 ttgctatagt taagaatcta gtaagtcttc atggtggaga tatagaagta gtaagtgaag
    28621 tgaataaagg cacagagttc ttaatttcac ttccaatgaa gggatttagt agtgaacatt
    28681 acacagaaga taagagaata tataatattc aagaaaaaat aaaaatagaa ttttctgata
    28741 tatattatta aaaatgatta tgagcaaaaa taaaataaaa atactatttg tttaaatatt
    28801 taagttgtaa gaaataggct atcataaggt aaattattat gatagcctat ttttagctag
    28861 gttttatatc tatatgtgag aacatataat ctctaaatta ccttcaaaac taaaatctcc
    28921 aaagtcgttt ggtagtataa aatgcatacc cttttttaaa ttgtattcta cagaattgtg
    28981 tataagtttt ccatttccat ccaacacact gtataaagaa aaaggtgtat tgtgtgtaaa
    29041 attacatttt ccaaaaacat ctaatttata aacactaaaa aattcgtttg acacaaatgt
    29101 tgtgcattta aagtctgatg ttgatacgat tttatagtca gtatcgaaat ttatatgagg
    29161 aacattcgta acatctattg atttttgaac atgaagttca cgcttattac ctgaattgtc
    29221 agttctatca taatcataga ctctataagt agtatctgag ttttgttgag tttctaaaat
    29281 caatgttcct ttgcatatag catgtatagt gccacttgga acatagaaaa agtctccctt
    29341 ttttatatta acctttctaa gtagtttgtc ccattcatta ttatttacca tatttacaaa
    29401 ttctttatga gattttgcat tatgacctat tataatttct gcatcatcag aacaatctat
    29461 tacataccaa cactctgtct tacctaactc tccattttca ttttttttag catattcatc
    29521 atttggatga acttgaactg ataaattatc atttgcatcc aatatttttg taagtaaagg
    29581 aaacttatca ccaggtgtgt ttccaaataa ttctctattt ttattccaca attcagatag
    29641 ttttttacct ttatattttc cattttctat taagcaatct ccatttttat gagagcttat
    29701 agcccaacat tcaccagttg ttggcgaatc tatttcataa ttaaatttat ctttaagtgc
    29761 agtaccaccc catattctat ccataaaaat aggttttaaa aataatggtt ccataatcct
    29821 gttcctccta tgatattaaa tttatcctac aaataattat atataaatag attataaaat
    29881 caatagtaaa aaagttttgt cccttgaaat gacataaagt ataaggagtt attaaataag
    29941 ataaactatg atatataata taaagttcta aattggagtg ataattatgg agttgtttaa
    30001 ggcagaaagc aaaaaatata aaaaagaatt tttaaaatta ttggagcaat gggttagtat
    30061 accttcattt tatgatagaa aaactgtatc taaagacatg ccttttggaa aaggagtata
    30121 tgatgcatta aactggtttg aaaatctagg tagagaaaac aatttcaaag taaaaaatat
    30181 agataatcat gctgttcaaa ttgaatatgg aaatggtaaa gagtatgtag atatatttgg
    30241 acattgtgat gttgtaaatc caggtgaagg atgggatagt gaaccattta aactaaatat
    30301 tataggtgat aaattagttg ctagaggtgt atctgataat aaagggccta tgatagtgaa
    30361 ttttttagca ctaaaaatga taaaggattt agatattaat ttaaaaagaa aggttagatt
    30421 aatagctggg ggaaatgaag agagtggatt taaatgtata aagcattatt atagcaaaga
    30481 gccatatggt gtatgtggat ttactccaga tgctaaattt cctgtattaa atggagaaaa
    30541 aggtggagca ataataaagt taatttcaaa tatagacgat aaatctttat atataagtgg
    30601 tggaatagag tttaatacaa taccagataa agtttatata aagaatgtag aaaaattggg
    30661 taaagataat atatgttttg atattaacaa tatatcgata aattatgata atggcaatta
    30721 tattgtgtat ggaaaaggtg gtcattcatc taaacccgaa aaatcaataa atcctatatt
    30781 agctacaatt aaacttttgt cagaaaatat tgatgaaaaa tggacaaaag atttatataa
    30841 gttgataaat caagataata taaatggaaa cttatttggt ttaaatattg aggggaaatg
    30901 tggaatttta tcaatggtgc caacaataat aaatatagta gatggaaaat tagaagtagt
    30961 tttaagcgtt agatatccag aaatattaac cattgaagat ataataaaaa aatttaattt
    31021 gtatatggag caaaataata ttaataaatt tgagcttatt ggggaaaatt taaagcaagc
    31081 aaattatata gatagaaatt caaaacttgt aagaagtttg catgatatat atataaagta
    31141 ttctggagat ttaaaaaatg atgttagagt aactagtgca ggaagttatg cttctgaaat
    31201 gaataattca gtaatatttg gttgtgaatt tcctgatggt agctttggca atgtccatag
    31261 tgcgaatgaa tttgctagct tagatagatt tataactgca ataggtatat atgcagaggc
    31321 aataattaca ttatgtaata aaatataaat acaaaatgtc ccttatatag acatattata
    31381 tatacaatta aaattttact tgatatataa ttgaaatcaa agagaaggtg atacaatgat
    31441 aactaagaaa catgcaataa tagtaaagag tttaagcaat aaagatggat atatgacatc
    31501 aaacgaatta gctatcaaat tggatgtgtc tactaaaact attaaaagat atatagcaga
    31561 tttaaatagt gttttaagca aatatgattt ggtaatagct tcttctagag gtataggata
    31621 caagttaagc ggttctaaaa acaatatagc cagagctgtt aaagaagcaa ataaatatat
    31681 tgatggtttt ttagatgact ctgaagagtc aagaatgtca aatataatat gtatgcttat
    31741 taatagaaat tatatgagta tagaagcaat ggcagaagaa ttaaatctaa gtatagccgc
    31801 tataaataaa ttgtctagta agcttaaaag aaaacttgaa aagtatgact tagttataaa
    31861 atctaaacct tactatggaa gctatataca tggtgaagaa attaatataa ggcaattaat
    31921 tactgactat gcaataaaat tagatgaaaa aagtaaagtg aaggtgtttt tagatgacat
    31981 tagtgaaaac gatatacact gtatagaaag tattttagaa aagcacttaa gagaaaaaga
    32041 tacaataatt tcagataaag attttaatct tttgctttca aaaataatcg tttctgtatt
    32101 taggagtaag agaggtcata gtaacaatat aaacttaatg gatacaagtt atagatttca
    32161 taactactac tttatagaaa atctaatgaa agaaatatct gataaaattg gatttaaact
    32221 tattgaagat gaagtaattt atatttcaaa ttattctggt gtaatagcct ataaaggtac
    32281 acaaggaaga aaaaacacaa gtgaaattga ggaacgaata agtactgtta taagtagtgc
    32341 acttcaagat atattcctta tttcaggtag tgattataca aaagatgatg agtttatggt
    32401 tgcaatctct gaccatataa agagattttt aaatagagct agagctaatg taaaatcaaa
    32461 taatccttta cttcatcaaa taaaagagaa gtttcctata gcttttaatt tagccgtgtt
    32521 tttatcaaat aaactagaaa ctgagtttaa tttaaaactt gatgaagatg agcttggata
    32581 tatagctatc cactttgctg cttcaaatga aagaatgaaa aaaaatacta gtaagaaaat
    32641 ttgtatagtt tgtcattatg ggataggaac aggacaactt atttctgaaa agctaaaaca
    32701 gaatataagt gatttaagtg ttgtgggtgt ttatccagtt agatatctcg atatggctat
    32761 aagtcaagat gttgatttga tagtatctac agttgaatta aaaggatatg aaaaacctgt
    32821 cttatatata gaaaatatat ttgatgatag cttaattgaa aacgtaaaca aagcctttta
    32881 tgaaaaggaa gaaagaagaa agataataag taacatgttt gatgaaaaag cttttttcag
    32941 tataaaggct agtactaaag aagaggtcat aatttcactt tcaaataaac taaaagaaag
    33001 agattttata gaggaaagtt ctataaaaag tataatagat agagaaaata tttcatcaac
    33061 tgaaatagga aatctagtag caatacccca cacaatagtg aagggagata aaaaatctat
    33121 aataggagtt ggaatacttg aaaatccaat aatttgggat aagcaagagg ttcagctagt
    33181 atttatggta ttttttaata ctaaagaaaa acataatttc tcgatattta aataccttta
    33241 taattttata aaagatgaag gaggagtaag aggaattatt aaaatatgtg actttaataa
    33301 attaatggca cttataggta attaaggagg aaattaataa tgggaacaaa catatttaac
    33361 aaagaatatg tatttttaaa tgttgatgct aagtcaaagg ttgaagtttt aaaatttata
    33421 tcaaaaaaag ctaaagactt aaacttggca gaagatgaga gcttagtata tgaaggttta
    33481 atggctagag aagaacaatt tactacaaat ttaggagaat ctatagcaat acctcacact
    33541 aaaaatgatg caattgaaaa tccagcagta gtagtattga agtttaatga agatgttgtt
    33601 tggaatgaag gtgaggataa ggttaaatta gcaataagct tacttatgcc aggaaaaagt
    33661 aaagagaata ttcatcttaa attattatca tctctttcaa gaaaattaat aaataaagaa
    33721 tttaaagatt ctcttttaaa aagtgataat gttgaagaaa tatctaactt aataaatgaa
    33781 gctttaggtt tataattaaa cactaaggtt ataaaataaa aaaataatta actaaaaata
    33841 ggaggaattt gaaatgaata aaaaattagt agcattatgt gcttgtccaa tgggactagc
    33901 tcacactttc atggcagcag aagcaataga gcaagcagca aaggcattag gttatgaagc
    33961 aaaagttgaa actcaaggtg ctgatggtgt acaaaatgag ttaacaagag atgatatatt
    34021 aggagcaact atgataatac atgcagtagc aataacacca gaaggtatgg aaagatttga
    34081 tggttgtgaa gtttatgaag ttgaattaca agaagctata aaaaatgcag aaggtgttat
    34141 aaaagaaata gaagaagatt taggaatata ataaatttat tgtggaggaa atagaatggc
    34201 aattaagaaa aaagttataa acagttcagg tgcttctaca acaggaggaa ataatgtagg
    34261 aaaatctact ccatctagtc caactcaatc aaagggaaat ggaaaatgga aagaaataag
    34321 taaacatata atgacaggta tatcatatat gataccagtt ttagtaatgg gtggacttat
    34381 aggggcctta tctcaattaa taccatatgc tatattagga cttgacccat ctgtaggtat
    34441 agttgatgct atgaactcag gagaatttac agggttcaag cttagtcttt taaatatagc
    34501 acaattaatg tcaaatttcg gatttacttt gtttggattt gcgataccac tgtttgcagc
    34561 attttgtgca aactctatag gtggaaaaac agctttaatt gcaggattta taggtggata
    34621 tatagctaat aaacctgtag gtgtaccaca attcgttgat ggacaatgga ctgaagttgt
    34681 tccagttgca tcaggatttt taggtgcaat actaatagca tttataatag gatattttgt
    34741 aaaatggtta aataaatcta taaaagtttc tcataactgg ttagctttta aaacaacatt
    34801 tttaatacca ttaatagcgt cattagcttg tatggtattg atgatattta taataactcc
    34861 atttggtgga ttaattaatg aaagtatgaa aaacttctta acagcagcag gagcagctgg
    34921 agaatatgta tatgcaacag ctttggcagc agctacagca ttcgacttag gaggaccaat
    34981 aaataaagca gcaggattcg ttgcacttgg tttaactaca gagaatgtac ttccaataac
    35041 agcaagaaca atagctatag ttgttcctcc aattggatta ggattaacaa cattattaga
    35101 taaaagatta gtaggaagaa gagtatatga tagacaattc tatcaagcag gaaaaacttc
    35161 tatattctta tcatttatgg gtatatctga aggagcaata ccatttgcac ttgagagacc
    35221 aggttttgtt atacctttaa acatagttgg aagtgtaata ggagctatta caggtataat
    35281 tctaggagca attcaatggt tcccagagtc agctatatgg gcatggcctc tagtagataa
    35341 cttatttgga tatataatag gtatagcagt tggtgctata ttcatagcag taggaaatat
    35401 attctataga aataaattaa taaaagatgg taaacttgtt gtagattata tcgactagta
    35461 tttaattttt actatctata aataactttg gctggtaata tggttttaat caatattacc
    35521 agccttacct atatgattaa ttaaaggcgg tggagataag atatggataa gatagaaaaa
    35581 gtaagagagt attttagaga atatgatata gatggatttc ttataaatag tcctacaaac
    35641 aagttttatg ttggaaattt atttagctct tcaggttatg tttttataac taaagaatca
    35701 caatatataa tagtagattt tagatatttt gaagaaatta aaagaaaatc tagtttgttt
    35761 aatgtagtat taatggacaa aactagaact cattttgata taattaatga tatttgtaga
    35821 gagcagaata taaaggaaat tggatttgag ggaaatgaag tatcttttga tttatataga
    35881 agtatgagta ataagttaag tgcaacctta aaatcagttg atttatctac attaagggaa
    35941 acaaaaaatg aagacgaaat aaaatatata aaaaaagctt gtgaaatagt tgatgctaca
    36001 ttttatcata tagtagactt tataaaggtt ggtatgactg aaaagcaagt tgaaaatgaa
    36061 attgttagaa ttataaaaga tttaggtggt caaaaagaat ctttcgatac aatagttgca
    36121 tctgggctta gaggagcatt acctcatgga aaagcaagtg aaaaagttat agagtatgga
    36181 gattttgtta cctttgactt tggtgctaag tataataatt attgttcaga tattaccaga
    36241 accatttgta tgggtactat aaataaagag ttagaagaaa tatataatat agtaagaaaa
    36301 gctaatgaag agtgtataag ggttttaaga ccgggaatga ctactggaga gatagataaa
    36361 gttgcaagag acataattgg ttcatatgga tatgccaata attttggtca taatctgggt
    36421 catggtgtag gaattatggt tcatgaatat cctgcattag ctcctgaatc aaatgaagta
    36481 ttaaaagaag gaatgattgt aactatagag cctggtatat atgttccaag tcttggaggc
    36541 gtaagaatag aagatgatgt acttataaca caagatggat gtatgagatt gactacatct
    36601 actaaggatt taatagttgt aagctaaaaa taaattagat aaatatttta taacaataag
    36661 aaaagaagag gtaaatttaa atgtataatt ttgatgataa accagataga gtaagtgaaa
    36721 agtgtagaaa atgggattta aatattataa gagataaatt tggagatatt agagaagatt
    36781 ttataccaat gtggattgct gatatggatt ttaaaatacc aacagaaata gaaaacaaat
    36841 ttattgaagc tgtaagaaga ggtgtatttg gatatacata ctgttatgat gaattttatg
    36901 attcagttat tggttggcaa aaaaatatgc atgaggtaaa tgtagaaaaa gaatggatta
    36961 cattaactta tggaactgta tcaacacttc attatgtagt acaagctttt tgtaagaaag
    37021 gtgatagtat aatattaaat acaccagttt atgacccctt tgaatcatca gcaaaaaaac
    37081 aaggtgtaaa tgtaatttgt aatacattag atgttgtaaa tgatagatat tatataaatt
    37141 ttgataaatt agaagttcaa attaaagaaa ataagcctaa attaatgatg ttttgtacac
    37201 cacataatcc atctggaagg atatggacaa ttgaagaaat gacaagggta gcaactatat
    37261 gtaaagaaaa taatgttatt ttggttgcag atgaagtaca tgcagagcat attcattatg
    37321 gtacatttaa ttcaatactt aagattggaa aagagctttt agaaaatgtt attctattga
    37381 catcaccaaa taaaggattt aatcttggtg gattaaagac ttcttattca atagttataa
    37441 ataaggatat aagggacaaa tttagaaata aattaaagca aaattctata acttctccaa
    37501 atgtgtttgg aataatagga ttgataaccg cttataatga atgtcatgag tggttaagtg
    37561 gtgtaaatga atatataaaa tcaaattatg aacttttaga aacttgggta aataaatata
    37621 ataagataaa attgatgaaa atggaatcat cttacttagc ttggatggat ataagtggat
    37681 taggtattag tgcaagtgag tttacagata aattggctat agatacagga gtattattag
    37741 aagatggaag tcattttgta aaagatggtg agaagtatgt tagaataaat ttaggaactc
    37801 aaaaggaaaa tgtaattgaa gccttaaata gaatggattt atttttaaag tctttataaa
    37861 aatttggttg aattagtatt ttatataaat ttaaactatt aatatggtat atacataagt
    37921 agaatttgtt ttagattact taagtatata ccatatttta tataaaaatt aaatttgcat
    37981 tagtcatgtg ataactattt aaattcatat gtattttgta tgtataatag atataattat
    38041 tatttaaact tattgtttat ttaatttttc aatagaaaaa gatttatctg taagtttaaa
    38101 attatctttt atacccttag ttaagtatac tttcttatcc ttagttatga atatagcatc
    38161 tacattatca attttattaa ttaaattcat acctttttca agaccaagac caaatgtaga
    38221 agtggataag gcatcacaat taatagattt atcagagatt attgtaacac tacttaaatt
    38281 gttttcaaaa ggataccctg taaatggatt taacatatga tgatatattt ttccgtcttt
    38341 ttcgataaat ctttcataaa ttccagaagt aacaacagac ttatttgtag tttctatgtt
    38401 tcctatggat tcaccatttt tactagttgg gtcttgaata cctattttaa aaggtttatc
    38461 atttttacca ttttctaaag taaatatatt tcctcctaag tttactaaac ctttttttac
    38521 atcctctgat tttaaatagg ctacaatttt atcagcagca tatcctttag ctatggcacc
    38581 taaatcaatt ttcatatttt ctttggttaa ttttatagat ttattttttt catccaatat
    38641 gatgttctta taatcaataa gaggcagaag tttttgtatc ttagaatcac ttggaacttt
    38701 agctttatct gttccaatag cccataaatt tgataatgga ccaattgtta tgtcaaaata
    38761 tccatctgat aatttggaat attgtatagc ttcttttacg acgaaaaatg tatcatcaga
    38821 aactctgaca aattttttac cagcattatc atttatttta gaaacatcac ttcctggtat
    38881 atgagtgctc attttgttgt caatatatct taaaattgaa tcacattcat ttaagatttt
    38941 atcacttttg gattctttta cattaaatac agtaacttcg tttactgtac caaggtagta
    39001 gctagtttta ctatatttag tattctggct tgaactccta aaaaatgaaa ataaaattaa
    39061 tgctaaaatc aatatcacaa aaataaatgt gatttttttc ttattcatgg tatattcctc
    39121 ctatgaaatt gtgttattat tttattataa cataatttca taggaggaca aaaaatgaag
    39181 aaaaaagaca ttcttcttat tttaggcatt ttggttgtta ttacagcatg ttatgggata
    39241 ataaatgtta taaattctaa aaatgctgga aatattgaga tttatgtaga taataaattg
    39301 tataaaactg tttcaataaa tgccaaagaa gaatttaaaa tagagagtag aggggggtat
    39361 aacatagtaa agattcatga taaaggggtt gagatagtag atgcttcatg ccctgataag
    39421 gtttgcgtac atactggatt tataaacaag ccaagtcaaa gcatagtttg tatacctaat
    39481 agagtgagca taaaaataaa tacaaatgaa aagaatgata atcaagaaga tataatttct
    39541 aaataaaata ataaaaataa ttgaaaggaa ttggtgataa ttatggttaa cttaggaaac
    39601 gattgggatg aattattgaa agaagaattt gaaaaagatt actacttaaa cttaagaaaa
    39661 tttcttatag aagaatataa aacacgccaa attttcccaa atatgcacaa tatatatgaa
    39721 gctttaaaac acacttcata caaagacaca aaagtcttaa tattaggaca agacccttat
    39781 catggagata atcaagctca tggtttagct ttttcagttc aaccacaagt gaaaactcca
    39841 ccatctttat taaatatgta taaagaatta aaagatgatt taggttgttt tatacctaat
    39901 aatggatatt taatgccttg ggcagaccaa ggagtgttac ttttaaatac tgctcttact
    39961 gttagagcgc atgaagctaa ttctcataag aacaaaggat gggaaatttt cactgataga
    40021 gtaatatcta ttttaagtga gcgtgaagac ccagttattt ttgtactatg gggaagtaat
    40081 gcacgtaaaa aagtagagtt gattgataca agtaaacatt atattttgga agcacctcat
    40141 ccaagcccat tgtcagcaag taaaggcttt tttggatgta aacacttctc taagataaat
    40201 gaaattttga aaaagctagg aaaagaacct attaattggc agatagaaaa tatataaaag
    40261 taaaaggtaa caaaatgata acaaaacaac aataaacaat gacattgttg taactaatag
    40321 aaatatgctt aaatagttga aaatactatg tatgcatatt tctatttttt tgtcaagttg
    40381 acaactcttg aaaaaatagt tacaatttag ttacacaaaa aacacaaata aaaaaagaaa
    40441 cttacaaagc agaattttat gaagcaaaat atatagtgta aaaaatctta gggaggaaat
    40501 tttatatgaa ttttaaaaaa ttgagtaatg ctaaagtctc ggtaatggca gttgttttat
    40561 cagttggaat attatcggga tactctgcgc ttaataaaca agtaacatta gtagtaaggg
    40621 gagaagaaag aactatatca acatttagtt ctaatgtagg agacctttta gaatctcaaa
    40681 acattagcta tgattcaaat gatatagtaa ctttagatga aaatacaaag ttatctaatg
    40741 gagataaaat agaagtaatt gatgtaaagg aaaaaacagt tgtagaaact aaagacattc
    40801 catttgaagt tacagttgtt gaagatggct ctttattaaa gggtgattct aaggttaaag
    40861 aagaaggaaa gtctggtaaa agtgaattaa tatataaaga aacttatcac aatggaaaga
    40921 aagtagaaaa ggaatttgtg aaaaaggttg ttaaagctga gccagtaaat aaggttgtaa
    40981 gcaaagggac aaaggtagaa atgcaagtag cctcatcaag gggagaaagt tcaagaagaa
    41041 tggcttcatc atcatctagt agcagtagta gtaacaatgg taaaaatatg agagtggtag
    41101 caactgcata tactggtcac agtataactg caacaggaac aaaacctaag tggggaacaa
    41161 tagctgtaga ccctaaggtt ataccatatg gaactaaagt ttatatacct caatttgaca
    41221 tggtgtttac tgcagaagac tgtggtggag caataaaagg aaataaaatt gatatctaca
    41281 tgaatgattc aaaaacagtt tataactggg gaagaaaaac tatagatata cgtatattaa
    41341 aatagttaaa taatttatat ttttactgga aattatattt tgattatata gagaagaaac
    41401 tctttataga ttctaaaaat ataatttcca gttttttatt ttggatatgt aataaaacag
    41461 gtaaatttta accatgtata gataaaagct atttaaatta taatttacta tcatataaat
    41521 ctataaaaat tgttgcaaag agtcaaaata tatgatatta tagatttcgt tgagaaggat
    41581 ttcattatgg gagtggatgg gtgccggtgt gccctctagt cttcaaaact agtatgaggg
    41641 gttaagagcc tcttaggtgg gttcgattcc cacgcattcc cgccaatatg taaaaggtat
    41701 cttttaatta aaagatacct ttttaaattt attttaagat aatttatttt taataatata
    41761 taatgttttg tattatataa tgtaaatttt ctaatacaaa acattatata tttagataaa
    41821 aatctaggag tgtggaaaga tggaaaataa ttttacaacg agaactagtt ttctagttgg
    41881 agatgatggg ataaaaaaac ttaataattc aaatataata gtatttggag ttggaggagt
    41941 tggtagtttt actgttgaag ctttagcaag agctggagta ggcaatatta ctattgtaga
    42001 ttttgatgat gtagatataa ccaatataaa tagacaaata ccagccctac attcaactgt
    42061 aggaagatac aaagttgatg tcatggaaga aagaatatta gatataaatc ctaatatcaa
    42121 tataaaaaaa ataagaagtc tttataataa agatactagt gacgaaatac taactgaaag
    42181 atatgattat gttgtggatg cgatagacat ggtaagttcc aaaatacatc taattgaaac
    42241 ttgtgaaaaa aaaggattga aaataataag ttctatgggt atgggaaata aattagaccc
    42301 aactaaaata gtcgttacag atatacacaa aacaagtact tgtcctctag ctaaagttat
    42361 gaggaaagaa cttagggata gaggtatcaa aaagttaaaa gtagtatatt ctactgaaca
    42421 accaatagaa cttaagaaga aagtaatgaa tggtagaaaa gtaacacctg gtagtgtatc
    42481 atttgtccca tctgtaggag gtttaataat cgcatctgta atagtaaatg aactattagg
    42541 acaataagtt ttaaatattt taatttataa attttatata caaatttgaa ggctagaagc
    42601 atgaatacaa agcaatttta tattcatact tttagcctaa ttattttata ttaccttatt
    42661 ttatgtagtt gtagttttat tttttagatt atgtatctat ttcataatta tattttgtaa
    42721 atataatttc tccatcacta taaaaaacaa tatctcctat tctatctgtt ctataaattg
    42781 atacatggtt ttgttttaga ttattaataa cctccctatg tggatgacca tattgatttt
    42841 tatatccaca tgatattgca gctatatctg gactaatttt ttttataaat tctaaagatg
    42901 aagaagaatt acttccatga tgacctattt ttataaaatt aatattattt aatttaaaag
    42961 aatgtaaaat ttctttttca ttttcttctt cagcatcacc cataaataaa aaagaattat
    43021 cattaaaagt taatttgaaa actatagaat ttaaattaga ttcttcttgt atatatgatg
    43081 gacttaatac atatatattt atattgttat ctatgtttaa aacatcattt ttatagagat
    43141 gctgaataga taagttttta tccgtacaag aatttattag attttgataa gccttactat
    43201 cagttgattg ttcaggcata tatatggagt ttacattaaa tttttttata atattatcaa
    43261 gacttcctat gtggtctgaa tctggatgag ttgctattat aatatcaata gtttttattc
    43321 ttttttgtct aagataacta ataattatat tttcgctatc ttcatcacct ccatcaatta
    43381 atatattctt atttgtgggt gtttgaacta aaatactatc accttgtcca acatcaatca
    43441 tatgtataga tagtagagat tttttgtcac agccgattaa aagtgatatt ataataagta
    43501 ttgataaaaa aattttattt ctcaataaaa aatcactcca ttcattacat gtttatataa
    43561 atttatgatg ggtaatataa gatatatatg acttattata ttttatattt taaacaatat
    43621 aaatgaaatt aataccataa taaaattgga gttttttata tatacaaaat aaaattttta
    43681 aaattaagca ataaaaaagg taggaggatt ttatgttttt caaagatatt gaactaaatt
    43741 caaaaaaaga gttagaccca tattttgatt tagtagatta tgaagcttgt gagtactgtt
    43801 ttagtacttt atatatgtgg caacatgttt ataagacagg atattatata ggtgaagatt
    43861 ttgccgtttt agttggggaa tacgaaggag atagcttctc aatattacca cttgctaaaa
    43921 aagataaact tccagaagtt gtagattttg tccttgaata tttttctaaa aataataaaa
    43981 aaatatactt aagaggtatt actactgaag tagtagagtt tttaaaagaa aaatatccag
    44041 gtagatttga gtatatagaa gaaagagatt tatttgacta tatttatgat gcagaaagtt
    44101 taagaactct agctggtaaa aagaatcaaa agaagagaaa ccatataaac tatttcttaa
    44161 aagagtatgc aggtagatat gaagctaaat tattagataa agaaaacttt gatgagtgtt
    44221 tagtactaat gaaggaatgg gaaagtaata aggaagaaaa taatgaattt gatgaaagta
    44281 tggatgatga attaataggt atcaaaaaga tatttaacca ttatgacata ttaaaagata
    44341 aggtaaaagt gtttggtgtt tatgtagatg gaaaattaga agcttttagt ataggagagc
    44401 ttttaaatcc aaatatggca ttgattcata tagaaaaagc taatcccgat ataagaggtc
    44461 tgtatccatt tataaatcaa caatttttgg taagtgaatt taaggacgtt gagtttgtaa
    44521 atagagaaga agatttaggt attgaaggat taagaaaagc taaactttct tatcatcctt
    44581 gcagatttgt tgaaaagtac agtgttaggg aggcttaata gactatatgg agattaggta
    44641 tgctaaagaa gaagagatag aaagtataaa agatatttgg agttattgtt ttaacgatac
    44701 ggagagtttt atgaagtact attttaatga taaatataaa tctgaaaata ctgtagtagc
    44761 tttagatgaa ggaaaaatta tttcttctct tcagctaaat cagtataagt tattactaaa
    44821 tagcaaggta tataatacat catatgtggt aggagtttct acacttccag agggaagagg
    44881 agctggatat atgaacaaag taatgaaatt tactttaaac gaattgtata aaaaagggca
    44941 attagtttct atattgatgc ctattgacta tagactttat agaagatttg gatatgagca
    45001 ttgttacgac cagattgaat atactattaa cacagatgat ttaaaaaatt ttaagtcaag
    45061 tggaaaaatg ataaagtcta atttatcaca aattgatgat ttgatacaaa tagatagaac
    45121 ttttttaaat gaggttaatg gtaatgtgtt aaaagatgaa cattattatg aaaacttatt
    45181 taaagagatt caaagtgaag atggattttt atacattcat gaaggaaatg aaaaagatgg
    45241 atatatagtt tattttcttc aggaagataa aatgtttgtc agagagttat tttataaaaa
    45301 tatagatgcc cttaaatcaa tgcttaaatt tatatataat cacaatactc aatgtaagat
    45361 agttactata tctactccta caatagataa gataaggttt atattagaca accctaaaga
    45421 tagtgatata aaaataaaac cttttatgat gggaagagtt ataaatgtaa agaagtttat
    45481 tgaagatata gatattgaga aggatattaa tagctcattt aatttattaa tagaagacaa
    45541 gtttatagat gaaaataatg gtctatttaa aatttctata cagaataaga aggtatctgt
    45601 agagcagtta gataaaaaag gtgcagaaaa gcctcaagaa gattttgata ttaaattaga
    45661 tataaatacc ttaacacagt tatcattttc ttatatagat gtaaatgaag ctattttctt
    45721 aaatgatata aaagatgttt cagaagaaac actagaaaca cttaattgta ttttttctaa
    45781 aaaaaataac tatataaatg aatatatata ggtagagtac gttaaaagat tctaaaaatc
    45841 ttttgacgta tttttttgtt tagataacaa tttatcttgt ttaaaaattt tctatttgaa
    45901 aataatctaa ataatatact tttatatggg agtgattata attcaaataa gaagaccatt
    45961 attaattata ttaatgtttg tggtagctat atcatttatc attacaaata acacaaaaaa
    46021 cgacgaagaa cttaatgata agataataac tatacaagga gttgtcaaag gaaaaattga
    46081 aaagaaaaga tacaatcagt acaaggtagg ggtttttcta ataaatgatt atacaaaaaa
    46141 taaaaatttg aagattggtc agaaagttaa tattacaggt aaattcaaaa gtttagataa
    46201 aatgaagtat gatgattttg attatggaag gtatataaaa agtactggat ataaaggtat
    46261 agtgtatata aataactata aaattattga taaaaataaa ctttactctt taatagggga
    46321 aataaagttt tatattagta aaacttatag gtacttatat aagaaaaact cagattttat
    46381 aaattctata ctattagcag aagttgaaaa tttgactgag gaacaaaaag aaatttttac
    46441 cagaactgga actagtcatg taatatctat atcaggtctt catacaggaa tattatgtgt
    46501 aattatatcc tttctattaa gaggtataaa taaattgtat aaattattaa tactgtcgat
    46561 ttttataact ttatattgta taatggtagg tgcgtctcca tccataatta ggtctatagc
    46621 atttgttatg attttttatt tatcaatatt tattgataga aagaaagatg gaatatcggc
    46681 attgtctcta attgggataa ttttaattat gaataacccc tatgttatat ataatgtaag
    46741 ttttcaattg tcttttttag ctacactttc tatattaata ttctataata aaataaatag
    46801 tattataaaa ttaagtatgg tatcattgac aatatcatca aatatattaa ctttacctat
    46861 aatttattat acatttaaag gtataccatt gctttcaatt ataggaaatt taattatagt
    46921 gccatttgtg ggagtaataa tgtatttaag tattgcaagt ttaatcgtat ttaaagtaag
    46981 tgtagtaata gctaaaatta tatctttttt taatagtaca tttatagaaa gcatattttt
    47041 tttactagaa aaaatcagca atttaagttt tgcatacatt aatatagaaa atcctaaatt
    47101 ttatattgtt gttatttatt acataggagt gtttttttac atattttaca tagaaggaaa
    47161 agagataaag gagcaagaga atgaattaca aggatattat aaggaatgta aaagagaaaa
    47221 actttgaaaa aatgtatctt ttttatggaa gagagtatta tttaattgag aatgcaataa
    47281 aagcatttaa agatagtcta aacgagggaa tgcttgattt taatttagat ataatagatg
    47341 ggaaggaaat agtcttaaat cagcttataa gctctataga aacacttcca tttatggatg
    47401 ataggaagat agtaataata aaagactttg aactattaaa aggaaagaaa aagaatttta
    47461 cagatagtga tgagaaatac ttaatagagc atttagacaa tattccagac accacaacta
    47521 tagtttttgt tgtatatgga gatgtagaca aaagaaaatc attagttaag aaaataggaa
    47581 acaatggaat tgtttttgat tgtgataagc tttctgatat ggatttgttt aaatggataa
    47641 aaaaatcttt tgctttaaat gatgttataa tagataattc tcaaatcatg tattttattg
    47701 aacaagaagg atacagagat aagagtagtg aaaagacatt atctgattta gaaaatgaaa
    47761 taaataaaat aagttcattt gttggtaaag ggaataatgt aaccaatgat gtaattgata
    47821 aattatctca aaaaaaagtt gagaatgata tattcaaatt aatcgattat attggagaac
    47881 aaaatgcttc taatgcaatg aaaatattaa atgatatgat acaagaaggc gaatctgtat
    47941 taggtatatt ttctatgata gcaagacaat ttaaaataat tatgcaagta agacagttac
    48001 aacttgatgg atattcaaca aaacttatag ctgataaatt aaaaatgcat caatttgttg
    48061 taggaaaagc attaaagcaa actaaaaatt tttctgatga tataattgtg gaaatattaa
    48121 actatatatt agaaagtgat tacaaaataa agacaggtct tataagagat actttagcag
    48181 ttgaaatgtt ggttagtaga tactgtaaaa gagaagcaat ttaaaaaatc tatatattaa
    48241 taaaaaataa atagcaagat atgttatagt tcagagtaga tgtaagctaa attaagtttg
    48301 atatacaatc aactataata tgtctaacta tttatttttt attgtataaa tttacaaaat
    48361 aaaaaacccc tacatagggg ttattatata taagaaatat aaactggtaa ttaagcgttc
    48421 ataccgttta atttttgagc taatttagct acttttctag ctgcagcatt cttatgtata
    48481 gttcctttag aagctacttg gtatattctt ttttgagcgt attggaactt agcaacagca
    48541 tcctctctgt ttccagctgc aacagcatct tcgaatcttc ttattgcagt ttttatttga
    48601 gattttctaa ctctatttaa agcagttttc ttttcgataa cacttattct tttcttagct
    48661 gattttatat ttgccaccgt gttcacctcc ctgtattttt ttatcatatt catcgtcaac
    48721 atattagatt ttaacagaaa taaatctaaa aatcaaggat tattatttat atattttaat
    48781 aatgtttttt attttgattt tttagactgt aaattgtgta taaataattt gcaatatctt
    48841 gtttttaaat tggtatattt tgtgttttaa atattttgac atatataatt aatatttcat
    48901 tttgtaacaa atatttgaga taatacaaac tattaacata cattttgata gtatttccaa
    48961 taattataag ataatacaag aaattactca tttttgtaaa tttaatgaaa aattataaaa
    49021 atataaattt atatgcttaa ttaagataat ttttccaata atcaagataa tttttttcat
    49081 tagtatgtat gatagatgca tcaaacaata taactaatat gttagaaggt tatatgtaat
    49141 taatgtcagt gtttatatag aatagcattt gtagcattta ttatagttgt aattactatt
    49201 tatatcatag acttgaatag tctaaaaaaa tagactataa tatttttata gactaaagaa
    49261 agggctgata agatggacat agcaaaacta attttaaatc ctgcaaggct tcgtatttta
    49321 caatatattc gactttatgg cagtgttcgt acatctgata ttgtaaagta tttaaatgat
    49381 attcctcgtg caacagttta tcaccatgta aaaattttag aagagaacaa tatgattgaa
    49441 gtaattaaag aaaatcgtgt gcgtggtact attgaaaagg tttatgcact aaaggaatat
    49501 acaacatcta tggaaggaga gaccttcgtt gcactttcta cagcatttta tgtaggtctg
    49561 atgcaggaaa tgaatgaata ctttagcaga aaagagcaag accataaaaa ggataatgta
    49621 tttttctctt cagcattact atatatgagt gataatgaat atgaaaatct tcttaaaagt
    49681 atagctgatt tattgaaacc atatatagaa caaaaaccta aatcaaatct aaaacttaga
    49741 aaactttcaa ttatatcttc tccacctgta gctgataatg ataaaacaaa ataacatgca
    49801 acccattttt ataaaaggag gttatatatg aaatttcatg agtttggaaa taaaaacaga
    49861 ccacatatta tgttgattca tgggggaggg aatgcatggt ggaactattt gcgacaagca
    49921 gaagttttgt ctaacaaata tcatgtgata cttcctacat tggatggtca tggagaagaa
    49981 tatgaaacaa catacatttc tacagaggat acagcagata agttgatatc atatatagat
    50041 gaaaattgca gtggacatct tttcgctttg tgtggggtat cacttggtgg tcaaattgtc
    50101 atggaacttt tatctcgaaa acctaatatt actaaaaaag caattattga tggtagtatt
    50161 tgctacccaa gacctcttat ggcaaggttt tgtattgctg gtattcgatt ttttggagga
    50221 cttttgttta gtgagaaggc ttgtcgcttt caaattgcta tgatgccaaa attgttacct
    50281 gaaaatatgc aatatccaga tgaaataaaa gcgtattata tgacagatat gccacatgta
    50341 agaaaagaaa cattctataa tatgtatcgt acttacatga tgaactatac acttaaagag
    50401 aatgttaaaa aaaccacagc acaagtaatg tactggtatg gagaaaaaga aatgaagtgt
    50461 gtcaaaaaat ctgcaaaact gtttcagtct tatgtgccct cttgtgagat ttatgaagct
    50521 aagagctata atcatgggta tttatcactt tatcttccaa atgaatggtt ggaaattgca
    50581 gaaccatttt tccaaaagga ataaaggtgt taggtgatta ttgtataatt attgctgttt
    50641 ttttatatag taaataaatt aggtataaat ataaaaaatt attttagtta atatattagt
    50701 gaaagcttaa taggaaaatt ataaatagat taaaggtagc acctatataa atagttgcta
    50761 cctttttctg tttgtaaata aatgatagat aagaaggttt actgacaaat cactgactaa
    50821 acccaatttt cttacattga aagccttaat tttacttgtt ttaaataaat ttacaatttc
    50881 ttgttataaa atactaaatt ttgttaacaa ttataattag catttaattt atactggagg
    50941 tttatatgat tagtgtaaga acagatttag ctttagaagc tagtgaaatg tgtgaaaagt
    51001 ctcaagaagg aagtagtatt cctggtgtaa aaatagaaac taaagaatta gaaaattgca
    51061 ttgtaactaa agttgaagta atagacaggc aaggcgcaga gataatgaat aaagatatag
    51121 gaaagtatat aactctggaa agtaatctta tgaagtttga tgatgatgag tcaagagaag
    51181 aaatgataag ttatttaaaa gaggagttag tagatatatt tggccaagac aaaaataaaa
    51241 aaaccctggt aattggtctt ggaaatagaa atattacttc tgatgcgtta ggaccaaaga
    51301 gtgtatcaaa aacacttgta actagacatc tttttaaaaa ttataataaa gactatgatg
    51361 atgatttcac tgaggtatca gctttaagcc caggagttat gggtgttact ggaatagaaa
    51421 ctagtgaaat agtaaaatca ttggttgaga aggttaagcc agatagagta gtagcaatag
    51481 atgcattggc ttcaagaaaa atggagagag taaactctac tatacagata tcaacagcag
    51541 gaatatcacc tggtggaggt gttggcaata ctagaaaatc tctaactaaa gaaactttag
    51601 gcgtggatgt tattgcaata ggcgttccaa cggtggttga tgcagcaact cttacaatag
    51661 atgtcttaga tatggcaata gacaatttaa tagctcaatc agaagaaaca gaaagttttt
    51721 atgaaatgtt gaaaaaactt aaagaagaag aaaaatatca tctaataaaa gattcgttag
    51781 acccatatga caagaactta atagtaacac caaaggatat agatgataca atcgaaaatc
    51841 tatctataat aataagtgaa ggcttaaata ggtctttaca tccaggtaga ttggtataaa
    51901 ataaggagga gcaattatgt ttaaaaaatg cattaaagta gtaaccttga cttttatatt
    51961 agtatgcatt ttaccaggaa aatcattagc tttgaatcaa gatgattttt taaagttttt
    52021 agtaaattca tcttatccag aagctaaagt cgaaggaaat gatacagaaa ataaaaagaa
    52081 taataaaaat aaagaaacaa gtaaggaaaa taaggaagaa agtaaagaag gaaacactaa
    52141 atctaaagat gcaagtaaag ttgataataa aaaagagagt gaaaaagaat atataaaact
    52201 ttatgttggt aaagaaaatg ttccagatat tgagtcaaaa aattcagaca caactgaaac
    52261 taacacaaca tctagttcag attataagga tgatttaaga gtaaccaaag aaaatccaag
    52321 aattttgata tatcatactc atggatgtga gacttattca aattcacctg atggaaatta
    52381 tcattcaaga gataagaaaa attcagtaat ggaagtagga agtgcattaa ctagtgcttt
    52441 agatagcaaa ggatggggtg tagtccatac tactaaatac catgactatc catcttataa
    52501 taactcttat gcaagtagct taaaaacaat acaaagtata ttacctaaat ataactcagt
    52561 ggatatagcg atagacttac atagagatgc tagggattta actaatccag ccactaagga
    52621 aaaagaccat ttaaaatata caactatgat aaatggagag agagtatcaa agttcttctt
    52681 tgttgtagga ggtaaaaata caaatagaaa acaacttaga gctttagcag aagatataac
    52741 tgcttttgct gagaaaaagt atcctggtct agtatctcca atagtagaaa aagactatgc
    52801 tagattcaat cagtttgcag ttaagaatca tatgttagta gaaataggga ataacgcaac
    52861 aagtgtagaa gaatctaagg ctactacaaa gtatttagcc gagattttgg atgaatattt
    52921 taaacaaaaa aattagagct ttaagttata attaggagca tgctaaatga gccgattaga
    52981 aaaaaatata gaaagtaaaa gtaaactaag aaaatacaag tatattatga gaatcttatt
    53041 tttgattact atgattatat gtacatcagt ttgtgtattt atagtggaca gtaatgcaaa
    53101 aagtatgcta ggaatgaaac aatatgattt tccaagtatt aaatttgata taacaaaatt
    53161 tgattttgac ataccaaatt ttgatataca tgaaatcata aaaaaattta ataagtagta
    53221 ataagtgaat aaggtttatt gattaaaata agccttattt tagttatata tttttaatgt
    53281 ataaaataaa aattcattaa ctgtatttta taggatttac tttaagttta tgatattata
    53341 taatatttag tgttagactt gataatccac atataatgtg gataattttg tttagtgcca
    53401 attagtttat attcaaaaat tttaaatttg gtatataaag agtttttaga taatgtaaaa
    53461 taataactat aagaataatc aagaggaggg aattttaagg tggacaataa acaaagtaga
    53521 actagaaatt ttagtataat tgcacatata gaccatggaa agtctacctt agctgataga
    53581 ttaatacaac aaactgggct tgttagtgaa agagacatga aaagtcaatt acttgacaat
    53641 atggaccttg aaagagaaag aggaatcact ataaagcttc aaaacataag attaatgtat
    53701 aaagcgaagg atggaaatga atattattta aatcttatag atacacctgg acatgtagac
    53761 tttaactatg aggtatcaag aagtttagct gcatgtgaag gagcactatt agtagttgat
    53821 gcagctcaag gtgttgaggc acaaacttta gccaatgttt atcttgctat agaccaagat
    53881 ttagaaatat taccaataat aaataaaata gatttaccaa gtgcaagacc agaggaagta
    53941 aaaaatgaaa tagaagattt aatagggctt gattcaagtg aagctcctct tatttcagct
    54001 aaaactggtt taaacataga agatgtttta gaagatattg taaaaaatgt tcctccacct
    54061 aaaggagata atgaagctcc attaaaggca ttaatattcg attcttacta tgatgcttac
    54121 aaaggtgttg tagcttatgt tagagtattt gaaggaactg ttaaaaaggg tatgacaata
    54181 aaaatgatga ataccaataa aaagtttgag gtaactgaag ttggtgttat ggctccaggt
    54241 caaactgaac ttagtgaatt atcagcaggt gatgttggtt atattgctgc tagtataaaa
    54301 gatataagaa gttgtcgtgt tggtgataca ataacagatt caaataatcc tacagaagaa
    54361 cctttaccag gatacaaaaa agctactcca atggtatatt gtggtatata tccaggagaa
    54421 ggagaaaagt atgagaatgt tagagatgct cttgaaaagc ttcaagtaaa tgatgctgca
    54481 cttgaatatg aagctgagac ttcagcagca ctaggatttg gttttagatg tggtttctta
    54541 ggtcttttac atatggagat aatgcaagaa agacttgaga gagaatttaa ccttgatata
    54601 attactacag ctccatctgt tatatataga gttacaaaaa tggatggaga ggttgtaatg
    54661 atacaaaatc ctgcaaactt accagaacca tctgagataa agatgataga agagcctata
    54721 gtaaaaggag atataatagt tcctaaagac tatgtgggtg ttgttatgga gctttgccag
    54781 gaaagacgtg gaaatatgtt aaatatggaa tatatagatg atagaagagt aatgcttcac
    54841 tatgaccttc ctctcaatga agttgtttac gatttctttg atgctttaaa atcaagaact
    54901 agaggttatg gttctcttga ttatgaggtt aaaggttatg tagcatctac acttgtaaaa
    54961 cttgatatat taatcaataa agagcaagtg gatgcactta gttttattgt ccatgagaca
    55021 cgtgctttcc ctagaggaaa ggcaatgtgt gagaaattaa aaggagaaat tccaagacat
    55081 cagtttgcta taccaataca agctgctgtt ggtaataagg ttattgcaag agaaacaata
    55141 agcgctctaa gaaaagatgt tcttgctaaa tgttatggtg gagatatatc tcgtaagaag
    55201 aaacttcttg aaaaacaaaa agaaggtaag aagcgtatga gacaaattgg ttctgttgaa
    55261 gttcctcaaa aagcatttat gtctgtgctt aaattagatg agtaaatcct tataaattta
    55321 gataaagata tttaaaagct agagtgattt tagaattatt tctaaattag tctagctttt
    55381 ttatggtata ttttaagtaa actcatagag aatataagta agaatattaa agaagaataa
    55441 aaaggtgata tttatgatta atatagaaat tcctaaaaaa gtagactata taattaaaga
    55501 attagaaaag aatggttatg aagcttatat agttggagga tgtgttagag attgtttatt
    55561 agaaagagtt ccaaacgatt gggatataac aactagtgct agaccagaag tggtagtaga
    55621 attatttgaa aaaacaattc caactggtat acaacatggg actgtaacag ttatgattga
    55681 acatgaacca tttgaagtga cgacttatag aattgatgga aattatagtg atggtagaca
    55741 tccagattca atagaattta caaataacat agttaaggac ttgtcaagaa gagattttac
    55801 tatcaattca attgcataca attcaaagac aggattagtc gacccattta atggctatga
    55861 agatatacaa aataagtata tacgttgtgt tggaaatcca gtagatagat ttgaagaaga
    55921 tgcacttaga atgcttagag cagttagatt ttcagcacaa ctcaatttta aaatagctga
    55981 aggtactaag caaagtattc ataaaaaagc agatttgata aaaaatgttt ctattgaaag
    56041 aatccagaca gagtttaata aaatattagt atctgattca tctaaattaa acttattaaa
    56101 atcaacaggt ttgttgaaat ttataattcc tgaaatatgt gaattagaag atgttactca
    56161 acacaatcca tatcacatct atgatgtaca aaaacatact ttaatagcta cagaagtaat
    56221 tgaagatgaa ctgtatttaa aacttacaat gttgtttcat gatttaggta aaaaagtaac
    56281 taaaacaact gataaaaatg gtgttgacca tttttatact catagtagag aatctgtaaa
    56341 gattgcaaag aagattttga aaagacttaa gtatgataat tatactatta ataaagtttt
    56401 aatattaata caatatcatg attatagaat agaacctaaa aggaagataa ttaaaaagtt
    56461 attaaataaa cttgaagatg ttgaattatt tgaagattta ataaaagtta attgggctga
    56521 tacacttgca aaaaatccaa agtatgcaaa acaaaagata ttaaatctta ttgagtgtga
    56581 aaaagaattt aaacatataa taaatcaaaa ggagtgcttt aatcttaaag atttagctat
    56641 taatggaaaa gatttgatat ctataggtat aaaaccaggt aaagatatag gatatatttt
    56701 aaataagatg cttgaaatag tcataaataa tccagagtta aatgaaaaag aaatactaaa
    56761 agaaaaagca ttaaatatat atacatttta gattcaaata taaaaaattt aattacttta
    56821 aaaaacatag aataaaatac ttatttagat aaaataaata atttttagag aatattttag
    56881 aagttacgat tgtatgattt ttaaatgcat aggcatttac tctaggacta tagactaata
    56941 taaaaaaaaa ctttgggaat actaaaacca taaatttata aaattaatat tttatatact
    57001 gtaaaacagt gaggagggac agtatgcata ctcaactaca aaagactatt ggtctttcag
    57061 cagctctttc tactgttgta ggtatggtta taggttcggg agtttttttt aagccacaag
    57121 caatatacac tacaactaat ggagctccag gtctaggtat tatagcgtgg cttttaggtg
    57181 gttttataac aataactgct gggcttacag ctacagaaat ttcagctgct ataccaaaaa
    57241 ctggtggtat gatgatttat atcgaagaaa tatatggaga aaagttaggt tttttaactg
    57301 gatggatgca aactgtatta tttttcccag gtacatcagc ggcattagga gtaatatttg
    57361 ctcaacaagc atctgaactt ttaggaatga gcccaaataa tatggcaaat gtacttccta
    57421 tagcaattgg agttatacta tttttagcgt tattaaatat aataggttca tccttaggag
    57481 gaaaagttca gacagtagcc actattggaa agatgatacc tttaatttta ataatagtct
    57541 ttggatttat taaaggtcaa agtagtgaag tattaaatcc atttgtagga gatggtgtaa
    57601 atgcatcaaa tgcacttgga caagtattaa tagctacttt gtttgcatat gatggttgga
    57661 taaatgttgg agctatatca ggagaaatga agtctccaga gaaagattta ccaagagcaa
    57721 tagttggtgg tttatcatta gttatggcag tttatattat aataaatata gcttatcttt
    57781 gggtagtacc agcaagtgaa ttagcaacag ttacttcccc agctacatta gtagcaacaa
    57841 gattgtttgg aaatataggt ggaaaagtaa taactgtggg tatattaata tcagtatttg
    57901 ggacattgaa tggatattta ttaacaggtt caaggatacc ttatacatta gctgaaatgg
    57961 gaactttacc agcatctaaa actcttttaa aagtaaattc tggaggttct cctgtaaatt
    58021 caatactact tataactgtg ttagcctgtg tatatgcatt atctggtcaa tttaatcttt
    58081 tgacagactt aacaatattt tcaatatggg tattttatgt attgactttt ataggtgtta
    58141 tgaggcttag aagacaaaag ccagatttac acagaccata taaagtccct ttatatccaa
    58201 taataccaat tatagctata ttaggtggac tgtttgtaat aataaatcaa atattgactt
    58261 caacagtcat atctttaggc ggaatattta taacactatt aggtttacca gtttattatt
    58321 atatgaaaaa aagataacta cttaatttaa gttagataaa tttgggtaaa ggtgaaaatt
    58381 aacttttatc caaatttttt atactgttat acaatataag tattatatat tgcttgatgt
    58441 gctattttgt ttgatttata atttagtaat tgatgtttta agataattta agaaatgagg
    58501 agtgaaagat gctaggactt tatgtacata ttccattttg tgtgaaaaaa tgtaagtatt
    58561 gtgattttaa ttcttataaa atggacatag attcaaaaaa aagatatata gaagacttaa
    58621 aaatagaaat ggaattatat agtaataaat tatataaaga taataaatat aaaaataaag
    58681 aatgctgtag tctaaataaa aatgacaaaa taacgagtat atttgtaggg ggaggtacac
    58741 ctagtatact aacctctgat gaaataagag aagtatttat aagtataaaa gaaatgtttg
    58801 atatagatga gaatgcagaa ataactatag agtgtaatcc tgggacatta acattagaaa
    58861 aattaaaaac tatgaaagag attggcataa atagacttag tatagggcta caagctattc
    58921 aagaaaagca tttgaacttt ataggaagaa tacatactta tgaagaattt gagaaaaact
    58981 ataaagatgc attaagtgta ggttttaaaa atattaatat agatttaatg tacagtcttc
    59041 caaatcaaac tctatgtgat tggaaggaaa ctcttgaaaa agttgtacac ttaaatccaa
    59101 cacatatatc agcttattcc cttatactag aagaaggaac tgaactatac aatatgtatg
    59161 aaagtaataa atttgaactt atagatgaaa atgtagatat agaaatgtat gagtatacaa
    59221 taaactactt aaagtcaaaa ggatataatc aatatgaaat atccaactac tcaaaagaag
    59281 gctataattg tgagcataat attttatatt gggaatgtga acattatata gggattggag
    59341 caggagcttc tggctatatt aatgagaaca gatacaataa tgtagaatct ttagaagatt
    59401 atcatttaag tttggtaaaa agagaaaaac caatacaaga aaatgaaatt ctttctgaga
    59461 aagatatgat agaagaaaag atatttatgg ggcttagaat gaataaaggc ataaaatttg
    59521 aagattttaa aaaaaaattt ggaatagatt ttagagagaa gtataataag caaatagaaa
    59581 tgctattagc tagaaagctt ataaatcaga gctttgaagg tatacaatta actcaaaaag
    59641 gaagagaaat atctaatagt gtatttatag aatttatgga ataattttta aggtgttgac
    59701 aaaataaatt tataatggta tataatatat ataatctttt tagcactcga acttagtgag
    59761 tgctaacagc taggaggtat aataatatgg aattaaatga aagaaaatta aatatcctca
    59821 aagctatagt taaagattat atagaaacag ctgaagctat aggttctaga acaatatcta
    59881 aaagacatga tttaggtgtt agtgctgcta ccataagaaa tgaaatggct gaccttgaag
    59941 aattagggta tttaattcag cctcatactt ctgcgggtag agttccatct gaaaaaggct
    60001 ataagttata tgtaaactct cttatgagta aaagcgaatt ggatgataat gataagattc
    60061 ttattgaaca atgtatgaat cacaatataa atcatataaa agaattgatt catgaaactt
    60121 ctaagctatt atcgcaacta acaaactata caactgtagc agttactaag agtttaataa
    60181 atcaaagtgt tataaagcac atacagttgg ttgctatgaa tgacaataat attttactta
    60241 tagttgtaac agataaaggg gacttaaaaa aagctaacct tacaactaat gtatatttag
    60301 atcaatctaa gttaaatttg atttctgata atcttacaag gaagctctta ggtaaaagta
    60361 taacagattt ggatgataat ctaattgctt ttattaaata cgaaataagt gagtattctg
    60421 ggcttataga tgagctttta aatgctttga actcaaatat gaaagaagaa gatttttctc
    60481 tatcattaaa tggagctact aacatattta gttatccaga atttaatgat gtattaaaag
    60541 caaagtcatt tttaaatatg ttggaaaaaa aggaaacaat agctgacata attaaatcaa
    60601 agggaataca aaaagacaat ctaaatataa taattggtag tgataatgat tgtgaactgg
    60661 cacaggattg tagtatagtt acagcaactt ataatgtaga tagagacttg gttgggcgaa
    60721 taagctttat aggccctacc agaatggatt atgctagaat ttactctata ataaattata
    60781 tgagtttatt aattaataga aagtaaagaa attttaaatt gcgaggtgta aagtcttgga
    60841 taaaaaaaat gagcaacagg aagtaagaga agaaaatgat acttctatta accaagaatc
    60901 ggaaactcaa gtagagttag aagaggaagt tgttaatgaa gaatgtgaaa catcaagtga
    60961 aaaaactgat gaaaaagaag ttgatgatga aaatgtaaca gatataaatt ctaagttagc
    61021 agaaaaaaaa ttgcaggatg agttggatga attaaatgat aaatatcaaa gacttcaagc
    61081 cgaatatgca aactacagaa gaagaactca acaagagaaa gagacaatag gcgtatttgc
    61141 aaatgaaaaa ataataactg aattgatacc agttatagat agtatggaaa gagccttaga
    61201 tgcatgtgaa gataaggaag atactatgta taagggtata agccttgtac ataaacaatt
    61261 aatagacact ttagtaaaat ttggagtaga agaaatagaa gcagaatcta aggaatttga
    61321 ccctaatcta catctagcag ttatgcaaga aagtgtagat ggagttgaag caaatcaaat
    61381 agtaatggta cttcaaaaag gatataagct aggaactaaa gttgtaagac cttcaatggt
    61441 taaagtttct tgctaagtat aaattaaagg ttaaattatt taacctaata taaaatatta
    61501 aaataagtaa aacttaatta tataattact cgaggaggat tttagtcatg ggaaaaataa
    61561 taggaataga tttaggaaca acaaattcat gtgttgcagt gttagaaggt ggagaagcac
    61621 aaataatagc aaatagtgaa ggtatgagaa ctactccatc agtagtagca tttacaaaag
    61681 atggtgaaag aatagttgga gaacctgcaa aaagacaagc agttacaaat gcagataaaa
    61741 caataacttc tataaaaact catatgggaa ctgattataa agtaaatata gatggaaaat
    61801 catatactcc acaagaaata tcagcaataa tattacaaaa attaaaatca gatgcagaaa
    61861 gttacttagg acaaacagta acagaagcag ttataacagt accagcttac tttacagatg
    61921 ctcaaagaca agcaactaaa gatgctggta gaatagcagg gcttgatgtt aagagaataa
    61981 taaatgagcc aacagcagca gctcttgctt atggtatgga taaattagac caagaaaaga
    62041 aaatattagt atttgactta ggtggaggaa cttttgacgt atctatactt gaaataggag
    62101 atggaacttt tgaagtttta gctacagctg gtaacaatag attaggtgga gatgactttg
    62161 accaaatagt aatagattat ttagcagagg aatttaaaaa agctgaaggt gtagatttaa
    62221 gaaatgataa aatggctctt caaagattaa aagaagcagc agaaaaggct aagaaagaat
    62281 tatcatcaac aatgagttca aacataaact tacctttcat aactgctaca gcagaaggac
    62341 caaaacactt aaatatagat ttatcaagag ctaaatttga agaattaact agaggtttag
    62401 ttgaaaaaac tatggaacca actaagacag ctttacaaga tgcaggactt tctacaggtg
    62461 atatagatga tgtattatta gtaggtggtt ctacaagaat accagcagta caagaagctg
    62521 ttaagaagtt tataggaaaa gaacctcata aaggtataaa cccagatgag tgtgttgcag
    62581 caggtgcttc tatacaagct ggagttttag caggggatgt taaagactta ttattactag
    62641 atgttactcc attatctctt ggtatagaaa ctatgggtaa tgtaatgact aagataatag
    62701 aaagaaatac tacaatacca actaagaagt ctcaaatatt ctcaactgct gctgataacc
    62761 aaacagctgt tgatatacat gttcttcaag gtgaaagaag tatggcttat gacaatacaa
    62821 ctttaggaag attccaatta acagatatac caccagcaca aagaggaata cctcaaatag
    62881 aagttacttt tgatatagat gcaaatggta tagttaatgt ttcagctaaa gatttaggta
    62941 caggaaaaga gcaaaaaata actataacat caaacactaa tttatcagaa tcagaaatag
    63001 agcaaaagat aaaagaagca gaaatgaatg cagaagctga caaacaaaag aaagaaaaaa
    63061 tagaagcatt caatcaagct gaatctacta tatatcaaac tgaaaaaact ttaaatgaac
    63121 ttggagataa aataagttca ggtgaaaaag aggatataga aaaagctata gcagacttaa
    63181 aagctgtaaa agataatcaa gatgctacag cagaagaact taagaaagct acagatgaag
    63241 taatgactaa gttccaaaaa gtatctcaag aaatgtacca aaaagcagct caagaacaac
    63301 aagcggcaca aggtgcagaa caagcacaag acaatggacc taaagatgac aatgtagtag
    63361 atgctgactt taaagaagta gatgaagata aataagctat ataatatgta acactaaatg
    63421 ataattggaa catatatgat ttgtgaaaat catatagatg ttaaagatat atggacaata
    63481 tacatcataa agaatataat atatcataga ttatataact taaagtagaa tataagaatt
    63541 aatgatagct tgtagtttat tattctacaa gctatcgttt tgtaaaatga aggtggtgtc
    63601 aatttttgag tacaaaaagg gattattatg aagtcttagg tatcagtaaa ggtgcagaag
    63661 ctcaagagat aaaaaaagct tatagaaaat tagcaatgaa gtaccatcca gatagaaatc
    63721 ctggagataa agaggcggaa gaaaaattta aagagataaa tgaagcttat gaagttctat
    63781 ctgatgatac aaagagaaaa acttatgacc agtttggaca tgatgggcta aatggtcaag
    63841 gcggttttgg tggtcaagga ggatttggcg gtcaaggctt tggtggtttt gaagatatgt
    63901 tcggagatat atttggtgac atgtttggag gaagcttcgg aggtggaaga gcaagaagaa
    63961 gagggcctca acgtggtgca gatataagac agagtgttac tatatctttt gaagaagcta
    64021 catttggtaa aaagatgtct ataaaagtaa atagaagtga agaatgtgaa gaatgtaatg
    64081 gaactggagc aaaaccagga acatcaaaga aaacttgttc aacttgtaat ggaactggtc
    64141 aagttagaac tgttcaaaga actccatttg gtaatatagc aagttcaaga ccatgtagtg
    64201 cttgtaatgg aactggagaa gtgattgaat ctccatgtag taaatgtcat ggaactggaa
    64261 atacaagaaa agttaagact atagaagtag atatacctgc tggtatagat gatggtcaaa
    64321 tgataaaact ttctggtcaa ggtgaagtag gagaaaaagg tgctccaaga ggagacttat
    64381 atattgtagt taatgttaag tcacatccac tatttactag agatggaaat gatatttatt
    64441 ttgaaatgcc aattacattt gttcaagcaa ctttaggtga tgagatagaa gtacctactt
    64501 tggatggaaa agttaaatat agtgtgccag aggggacaca aactggaact gtatttagac
    64561 ttaaagagaa aggaatccct agaattagag gtaattcaag aggagaccaa tatgttaagg
    64621 ttgtagttga aattcctaaa aaattaaacg ataagcaaaa agaactattg agagaatttg
    64681 cgaaggagtg tgggtcgaac gtccacgaaa agaagaaaac cttcggacaa aagatagaag
    64741 atatgtttaa gaaaaagtag ttaaagtagc caatatataa tcatattttt aaaatcttag
    64801 ttatttataa gattgtcaaa ggagatatta tgatttttat attggctatt tttgtataaa
    64861 aaataaatgt gcataaaata aaactgaata taaataattt tctgaaaaat acttgtccta
    64921 aaatatataa tatgctataa tataatcaaa taaaagaaag gggtgattcc agtgggaacg
    64981 gatgtggcac tgccaaatga tgaagaaaac tttattgtaa tatacaaaat aaaattagat
    65041 acttttagtg ctagattaag tactggaaaa actcatgagc tgggataatt attgtaatta
    65101 cctagactca aacttttagt agtaaaatag taatattata tagacaaggt atctaatttt
    65161 taaatctttt gtatgaatta aaatttttat aaagggtata tctgattaga atgtaattag
    65221 ggaaacctag ttgtgaatta cttttagtat atacatgtgt aaagaaatat gtatatggta
    65281 ttgggtattg tgctagatgt gtatgtaaag gaatatgtgt ctagttgggt atatgtaaag
    65341 aaatatactt ataatctatg aaaaattttt agtataaatt gctaaaacat tgagattgtt
    65401 ttttatagaa agttagttta taccaactaa aaatgtgctt aagatttgtt tgatgtttaa
    65461 ttataaataa ggttgtagat aaaaaaataa gtaaagacaa aggtttaaaa tacttgcttt
    65521 aaatatagtt tgttttgaac acttgccaaa ttaaaataat taataaaata gacatcttct
    65581 catagtaaaa tatactgtga gaggatgttt ttgctttgtt cataaacttt tatgaaaaat
    65641 ttgcatagtg tcacatttta atatactata tatataataa aatatagata aaattaaatg
    65701 attcaaaaga gaaaggtgaa aagagatgta ttatataggt gtagacatag gaggaacagg
    65761 tatacaagca ggagtagttg ataactatgg gaagataatt tttaggtcag agtgtaaaac
    65821 agtaattgaa aaaggatttg aaggaatact taatgacata aaaataatga tttataagtt
    65881 gttggaagat aataaactta ctatgagtga cataaaatct attggatttg gtgttccagg
    65941 ttttataaat aaagaaggat tagtaacttg tgtaaattta aaatggaata aaaaagcttt
    66001 taataaagag ttaaaaaaaa gatttccaga tgttgaaatt catggagaaa atgatgcaac
    66061 agtagcagca cttggagaag caaagtttgg aagtatgaaa ggtgctaatg ttggagtatt
    66121 atatacttta ggaactggtg taggtggagg aatagtaatt aatcaaaaag tcttttctgg
    66181 agcacatgga ttgggttcag aaataggtca tcaaattata ggagaaaact attttaattg
    66241 taattgtgga aataatggtt gtgtagaaac tttttgttca gcaactgcaa ttataaagta
    66301 ttctcaaaaa ctgatagaag aaggagaaaa gagtaggatt ttagatttgg cagagggaaa
    66361 tttagaaaat gtaaatgcaa agatggtgtt tgatgcatat agagaaaatg atttagtggc
    66421 aattaaagta ataaatagat ttaaagagta tttagcaaaa acttttgcaa acacaataaa
    66481 ttctttagac ccagaaatta tatctatagg aggaggcatt tctaaatcta gcgatataat
    66541 acttgatgga atagaaggtt tggtaagaaa atttgtatta tacaaaacag aagatatagc
    66601 tacaattaca tgtgcaactt taggttcaga cgcaggaatt ataggagcag cttttttata
    66661 atgtaaatga actgcttatt ggaggcgtga tgatatatgg gtaaaacaaa tgctagaaga
    66721 acgctttttt tgatagcaat aggttcagga actatgctga atcctcttaa ttcttctatg
    66781 atttcacttg ctttacatag tatacaaaat gattttcata ttagttttac aactgtatct
    66841 tggattgttt cagcatttta cctagctagt gctattgcac aaccagtttc aggtaagata
    66901 ggagatttaa taggtagaaa ggtcttattt ttgagtggtt tgatgcttgt aattttatca
    66961 gcatccatag taccacttgt tcaatcattc tttatattga tttttataag agtaattcaa
    67021 gcaattggaa caagtaccct atatccatct ggagttgctc ttatacaaaa taatataaaa
    67081 gaaagacaat cttctgcatt agcagttcta accatttttg cttcaactac agcaggatta
    67141 ggtccgacac ttggaggatt attgttggac ttaggaggat ggcatgctat atttcttgta
    67201 aatatacctg ttgttttggt ttctatatgc ttagggtatt ttttattccc aaaagaagta
    67261 aaagaaaaga aatctataaa agaaacttta aaaaatgttt tgtcaagatt agatattatt
    67321 ggcatacttc tattttcaat tgcaatgatt tttatattac tatttttgct atctatgaag
    67381 gaatctttta acttagaaca acttgtattt ggtataatac tgatgtgtct ttttatttgg
    67441 catgaattga gaacaaaatt gccatttata gatattaagc tttttgtaag taatcctaaa
    67501 ttgtcaaaag tttacttgca atttattata ttaaatttct ttaattatat tttgtttttt
    67561 ggtcttccta gttattttca agatgcctta cattatagtg caaagtcgac tggattattt
    67621 atgctattta tgtctggtat aggtatattt atatctccat taactggaaa atggatagat
    67681 aaatctggaa ctagatttcc tcttgttaca tcatcaatct ttatgtttat tagtgcagta
    67741 cttctttcag tagtttttat ccatatacct gtaataggaa agggtattat attatcatta
    67801 gcaggtatta gttatggagt tggtaatgta gccctgcaat cctctatgtt tgaagaaagt
    67861 cctcgtgaca gtatagggac agcttctggt ctgtttcaaa ctagtcgtta tttaggttct
    67921 attttagcaa ctgttttact tggaatggta tttgaagtaa atataacatc agaacaattt
    67981 cagatattag gttatgttat ggtggtctta ggtacaataa gttttttatt aaattttata
    68041 ttaaagaaag aattaagaaa agtagaatag tggatttatg gatttaataa gtctaataac
    68101 tgataagttt aaaaatattg tttgtaaaac ataactggtt tgatatttat taattctaag
    68161 ttttgaagca tagagtactc gataagtata caatattttg aattaaaatt aagcagaaat
    68221 ttgaactagt ctttcaaatc tctgcttaat ttagtagtta aattttttta tagagaaaat
    68281 aagatttgac acttagataa aaaatcattg cattttcaaa attctttaaa tcaaacccaa
    68341 gtatctcatt aatcttttcc aatctatata ctagcgtgtt tctatgtaaa aacatactag
    68401 tagatgtctt agaaatattt aaatcattat ttataaattt tattcctgta tatagaagtt
    68461 cttcgtcaat agatttaatc ttattaaaat ctattaattt attgagtata ttatcctcat
    68521 taattaaatc taaagtttta tatctaataa ggtcaaacaa atttaaaaac ttattttcag
    68581 cgttgtcttt tataatatga ggtagtaaat cagaacataa ctgtaatcta aaatcaaaat
    68641 cagctttact atctaattca ccaagataaa aaatataagt actttgataa gtttcagttt
    68701 caattatatt ttgtgtagtt acattaatat ctaagttata tttagaattt actataaaaa
    68761 aaccattgtg ttgtattata tttatatctt caaataaatt ataaaaaata ttttttagat
    68821 attcaaagtc tatatgagat tggtaaatta aataaaagtt gccttttaaa ttatcgagat
    68881 tatttttatc tagaaaatca ataacataaa tagcattatt gaaagtaact aaatggctac
    68941 tagcatctaa cttttggttt tcatatggat agatatttat ttttttatta agctgtcttt
    69001 ctaaaaaatc ttttaactct tccataaagt ctccttaaaa ataagattat attcttatct
    69061 tatagctaaa gtagtttctt tgtcaaatat gtgtactcta ctattgtcaa ctgaaagtgt
    69121 taagtcatca ccaacattaa ttctgttttt actgtcaaat ctaattgtta tcgtattttc
    69181 gcctagtttt aagtgagcat aagtttctgc tcccataagt tcttttattt caattcttga
    69241 tttaaaacaa gtatctggat tagatttaat aaaagtatct tctacatgaa tatcttctgg
    69301 tctgatacct ataacaactt cattattaat ataattgtct tttattaaat catagtgttt
    69361 ttctttattc aatttaagct taatatgttc attttgtgca taaatttcat cattttcttc
    69421 tattaatatt acatcaataa aattcatctg tggtgctcca ataaatcctg caacaaatat
    69481 attagctgga taatcgtata catcttgtgg agttgcaatc tgctgaacta ctccatcctt
    69541 cattactacg attctatcag ccatagtcat agcttcaact tggtcgtgtg ttacatagat
    69601 aaatgtagtt ccaagctgtt ggtgtagctt agttatctct gttctcatag cagttctaag
    69661 ttttgcatct aagttggata gaggttcatc cattaagaat accttaggat ttctaactat
    69721 tgcacgtcct aaggcaaccc tttgtctttg tcctccagat aatgttttag gttttttatt
    69781 taaaagaggt agtaagtcta atattttagc agcttcttta acttttttgt ctatttcatc
    69841 tttaggcaat tttctaagtt taagtgcaaa tgccatgttt tcgtaaacag ataagtgggg
    69901 atataaagca tagttttgaa aaaccatagc tatatctcta tcttttggtt ctatatcatt
    69961 gacaagttta tctccaatgt atagttctcc ttcagagatt tcttcaagac cagcaatcat
    70021 tctaagtgta gtcgatttac cacaaccaga tggtcctact aaaacgataa attctttatc
    70081 tttaatgtct atatttatat tttttactgc attaaatcca tttgagtaaa gttttgatat
    70141 attttttaaa attacttctg acataatatc ctccttgata tt
//
LOCUS       DAMJWS010000009        69131 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMJWS010000009 DAMJWS010000000
VERSION     DAMJWS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 69131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.009"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..606
                     /locus_tag="PC399_000619"
     CDS             <1..606
                     /locus_tag="PC399_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2591496.1"
                     /translation="ISGYLTKKLQDLNVDTIRTDILTSPTVTDVIVWNIEQSGTDTFS
                     ATYEVDQQIKEGEQTTTVKATYTVKVHVDADRDMVIIQNPTLAPAIEKSDYEPKTPEA
                     DASVDADTVNDATAFLETFFKLYPTATAKELAYYVFGNVLEPIGRDYLYSELVNPVLT
                     KDGDNMKVKVAVKFLDNQTKATQVLQYELILHKDSNWKIIM"
     gene            819..1676
                     /locus_tag="PC399_000620"
     CDS             819..1676
                     /locus_tag="PC399_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2591497.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVTSLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            1754..2335
                     /locus_tag="PC399_000621"
     CDS             1754..2335
                     /locus_tag="PC399_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2591498.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            2439..3284
                     /locus_tag="PC399_000622"
     CDS             2439..3284
                     /locus_tag="PC399_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591499.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            3281..3964
                     /locus_tag="PC399_000623"
     CDS             3281..3964
                     /locus_tag="PC399_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591500.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIICFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVEHM
                     WKSLGGVKL"
     gene            3961..4659
                     /locus_tag="PC399_000624"
     CDS             3961..4659
                     /locus_tag="PC399_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591501.1"
                     /translation="MKKILSSTIQIFKQIKSDPMMFAACFSPFIMGTLIKFGIPFLER
                     ITKFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            4671..5582
                     /locus_tag="PC399_000625"
     CDS             4671..5582
                     /locus_tag="PC399_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2591502.1"
                     /translation="MEKGRLLQNKNMNKAIQVSKITLIINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVVSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALNIFKESINKMVDHS
                     CDNATETRIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            5634..5774
                     /locus_tag="PC399_000626"
     CDS             5634..5774
                     /locus_tag="PC399_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591503.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            6065..6691
                     /locus_tag="PC399_000627"
     CDS             6065..6691
                     /locus_tag="PC399_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2591504.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            6813..7529
                     /locus_tag="PC399_000628"
     CDS             6813..7529
                     /locus_tag="PC399_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2591505.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            7679..8275
                     /locus_tag="PC399_000629"
     CDS             7679..8275
                     /locus_tag="PC399_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDF2591506.1"
                     /translation="MKILWIIIIMADNLLPLLLARFYPNYKHKEMALSVLGSRQSPVK
                     WIYNVWCIISGVVFCIAPYALYQENDSGWAIAIWMLLAIYGIGCEIISGVCPLNENRQ
                     DEDIITKIHGGTSAIGFMALLVVPLLFAILQFQTTKVVLGAISLVCFTIAFIFFCFFI
                     MGEKEKFANTVLRYGGLWQRLVLISCYIPLFVWSVSNQ"
     gene            8362..8553
                     /locus_tag="PC399_000630"
     CDS             8362..8553
                     /locus_tag="PC399_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDF2591507.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            8577..8807
                     /locus_tag="PC399_000631"
     CDS             8577..8807
                     /locus_tag="PC399_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591508.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            9074..9493
                     /locus_tag="PC399_000632"
     CDS             9074..9493
                     /locus_tag="PC399_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2591509.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            9496..9750
                     /locus_tag="PC399_000633"
     CDS             9496..9750
                     /locus_tag="PC399_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2591510.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            10125..10328
                     /locus_tag="PC399_000634"
     CDS             10125..10328
                     /locus_tag="PC399_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2591511.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            10406..11596
                     /locus_tag="PC399_000635"
     CDS             10406..11596
                     /locus_tag="PC399_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2591512.1"
                     /translation="MSKMKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPTGKRDDKSLREKEKQIKKDLDDGIDTIGGKMTVCQLYAKKNNQKKNIKRN
                     TEIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFHFKLSDVLEDDTEPKVILTPEQEEKLLSFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHE
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            complement(11853..12653)
                     /locus_tag="PC399_000636"
     CDS             complement(11853..12653)
                     /locus_tag="PC399_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591513.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(12848..14446)
                     /locus_tag="PC399_000637"
     CDS             complement(12848..14446)
                     /locus_tag="PC399_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2591514.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            14774..14950
                     /locus_tag="PC399_000638"
     CDS             14774..14950
                     /locus_tag="PC399_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591515.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            15124..16254
                     /locus_tag="PC399_000639"
     CDS             15124..16254
                     /locus_tag="PC399_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2591516.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            16495..16845
                     /locus_tag="PC399_000640"
     CDS             16495..16845
                     /locus_tag="PC399_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2591517.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            16853..17302
                     /locus_tag="PC399_000641"
     CDS             16853..17302
                     /locus_tag="PC399_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF2591518.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            17404..17979
                     /locus_tag="PC399_000642"
     CDS             17404..17979
                     /locus_tag="PC399_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2591519.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            17972..19381
                     /locus_tag="PC399_000643"
     CDS             17972..19381
                     /locus_tag="PC399_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF2591520.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            19565..20998
                     /gene="eutA"
                     /locus_tag="PC399_000644"
     CDS             19565..20998
                     /gene="eutA"
                     /locus_tag="PC399_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF2591521.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            21016..22380
                     /locus_tag="PC399_000645"
     CDS             21016..22380
                     /locus_tag="PC399_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF2591522.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            22393..23274
                     /gene="eutC"
                     /locus_tag="PC399_000646"
     CDS             22393..23274
                     /gene="eutC"
                     /locus_tag="PC399_000646"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF2591523.1"
                     /translation="MNEKDLKALVEQLVGQMLGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            23294..23947
                     /gene="eutL"
                     /locus_tag="PC399_000647"
     CDS             23294..23947
                     /gene="eutL"
                     /locus_tag="PC399_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF2591524.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            23958..24659
                     /locus_tag="PC399_000648"
     CDS             23958..24659
                     /locus_tag="PC399_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2591525.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            24662..26131
                     /locus_tag="PC399_000649"
     CDS             24662..26131
                     /locus_tag="PC399_000649"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF2591526.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            26227..26514
                     /locus_tag="PC399_000650"
     CDS             26227..26514
                     /locus_tag="PC399_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2591527.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGATAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            26641..27402
                     /locus_tag="PC399_000651"
     CDS             26641..27402
                     /locus_tag="PC399_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF2591528.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            27415..28044
                     /locus_tag="PC399_000652"
     CDS             27415..28044
                     /locus_tag="PC399_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2591529.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            28086..28811
                     /locus_tag="PC399_000653"
     CDS             28086..28811
                     /locus_tag="PC399_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF2591530.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDGFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMNGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDILNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            28824..29096
                     /locus_tag="PC399_000654"
     CDS             28824..29096
                     /locus_tag="PC399_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF2591531.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            29089..29640
                     /locus_tag="PC399_000655"
     CDS             29089..29640
                     /locus_tag="PC399_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2591532.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKICAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            29652..30743
                     /gene="eutH"
                     /locus_tag="PC399_000656"
     CDS             29652..30743
                     /gene="eutH"
                     /locus_tag="PC399_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2591533.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            30745..31218
                     /locus_tag="PC399_000657"
     CDS             30745..31218
                     /locus_tag="PC399_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF2591534.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            31731..32252
                     /locus_tag="PC399_000658"
     CDS             31731..32252
                     /locus_tag="PC399_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2591535.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            32631..33806
                     /locus_tag="PC399_000659"
     CDS             32631..33806
                     /locus_tag="PC399_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2591536.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDILKLGCEFMAKYAVEFLNKNNN"
     gene            33840..35378
                     /locus_tag="PC399_000660"
     CDS             33840..35378
                     /locus_tag="PC399_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2591537.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            35410..36612
                     /locus_tag="PC399_000661"
     CDS             35410..36612
                     /locus_tag="PC399_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2591538.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(36779..37378)
                     /locus_tag="PC399_000662"
     CDS             complement(36779..37378)
                     /locus_tag="PC399_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2591539.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            37548..38906
                     /locus_tag="PC399_000663"
     CDS             37548..38906
                     /locus_tag="PC399_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591540.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            38899..39531
                     /locus_tag="PC399_000664"
     CDS             38899..39531
                     /locus_tag="PC399_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2591541.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            39860..41017
                     /locus_tag="PC399_000665"
     CDS             39860..41017
                     /locus_tag="PC399_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2591542.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTLDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFILIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(41164..41799)
                     /gene="lexA"
                     /locus_tag="PC399_000666"
     CDS             complement(41164..41799)
                     /gene="lexA"
                     /locus_tag="PC399_000666"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF2591543.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(41903..43030)
                     /locus_tag="PC399_000667"
     CDS             complement(41903..43030)
                     /locus_tag="PC399_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2591544.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(43351..44196)
                     /locus_tag="PC399_000668"
     CDS             complement(43351..44196)
                     /locus_tag="PC399_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591545.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(44226..44597)
                     /locus_tag="PC399_000669"
     CDS             complement(44226..44597)
                     /locus_tag="PC399_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2591546.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(44750..45010)
                     /locus_tag="PC399_000670"
     CDS             complement(44750..45010)
                     /locus_tag="PC399_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF2591547.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(45199..46143)
                     /locus_tag="PC399_000671"
     CDS             complement(45199..46143)
                     /locus_tag="PC399_000671"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF2591548.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(46164..47018)
                     /locus_tag="PC399_000672"
     CDS             complement(46164..47018)
                     /locus_tag="PC399_000672"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF2591549.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKLLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(47033..48394)
                     /gene="accC"
                     /locus_tag="PC399_000673"
     CDS             complement(47033..48394)
                     /gene="accC"
                     /locus_tag="PC399_000673"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF2591550.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(48410..48862)
                     /gene="accB"
                     /locus_tag="PC399_000674"
     CDS             complement(48410..48862)
                     /gene="accB"
                     /locus_tag="PC399_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF2591551.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(49132..49578)
                     /locus_tag="PC399_000675"
     CDS             complement(49132..49578)
                     /locus_tag="PC399_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591552.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            49853..50131
                     /locus_tag="PC399_000676"
     CDS             49853..50131
                     /locus_tag="PC399_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591553.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(50260..51006)
                     /locus_tag="PC399_000677"
     CDS             complement(50260..51006)
                     /locus_tag="PC399_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591554.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEI
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(51135..51779)
                     /gene="trmB"
                     /locus_tag="PC399_000678"
     CDS             complement(51135..51779)
                     /gene="trmB"
                     /locus_tag="PC399_000678"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF2591555.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(51917..52237)
                     /locus_tag="PC399_000679"
     CDS             complement(51917..52237)
                     /locus_tag="PC399_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF2591556.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            complement(52690..52938)
                     /locus_tag="PC399_000680"
     CDS             complement(52690..52938)
                     /locus_tag="PC399_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2591557.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(52969..53430)
                     /locus_tag="PC399_000681"
     CDS             complement(52969..53430)
                     /locus_tag="PC399_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2591558.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(53468..54301)
                     /locus_tag="PC399_000682"
     CDS             complement(53468..54301)
                     /locus_tag="PC399_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2591559.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            complement(54575..55261)
                     /locus_tag="PC399_000683"
     CDS             complement(54575..55261)
                     /locus_tag="PC399_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591560.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            complement(55276..57753)
                     /locus_tag="PC399_000684"
     CDS             complement(55276..57753)
                     /locus_tag="PC399_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2591561.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            complement(57972..59375)
                     /locus_tag="PC399_000685"
     CDS             complement(57972..59375)
                     /locus_tag="PC399_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2591562.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            59868..60728
                     /locus_tag="PC399_000686"
     CDS             59868..60728
                     /locus_tag="PC399_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2591563.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(60967..61524)
                     /locus_tag="PC399_000687"
     CDS             complement(60967..61524)
                     /locus_tag="PC399_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2591564.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(61714..62601)
                     /locus_tag="PC399_000688"
     CDS             complement(61714..62601)
                     /locus_tag="PC399_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2591565.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            62720..63919
                     /locus_tag="PC399_000689"
     CDS             62720..63919
                     /locus_tag="PC399_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF2591566.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(64113..66668)
                     /locus_tag="PC399_000690"
     CDS             complement(64113..66668)
                     /locus_tag="PC399_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2591567.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWSLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(66665..67351)
                     /locus_tag="PC399_000691"
     CDS             complement(66665..67351)
                     /locus_tag="PC399_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591568.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGELL"
     gene            complement(67428..68336)
                     /locus_tag="PC399_000692"
     CDS             complement(67428..68336)
                     /locus_tag="PC399_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2591569.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(68338..69009)
                     /locus_tag="PC399_000693"
     CDS             complement(68338..69009)
                     /locus_tag="PC399_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591570.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
BASE COUNT        25217 a         9737 c        10600 g        23577 t
ORIGIN      
        1 atcagcggtt atctaacaaa gaaattgcag gacttgaatg ttgatactat tagaaccgat
       61 atactgacta gccccacagt tacagatgta attgtgtgga atatcgaaca gtcgggaacg
      121 gacacttttt ctgctaccta cgaagtagat cagcagataa aagagggaga acagacaaca
      181 acagtcaagg caacctatac ggtaaaagtc catgtagacg ctgacaggga tatggtaatt
      241 attcagaacc ctacccttgc accagcaatc gaaaaatcgg actatgagcc aaagacacca
      301 gaagcagacg caagtgtaga cgctgatacc gtgaatgacg ctaccgcatt tctggaaaca
      361 ttctttaagc tgtacccgac agccacagca aaagagcttg cctattatgt atttggaaat
      421 gtgcttgaac ctatcggcag ggactacctt tattcagaat tggtaaaccc agtacttaca
      481 aaggacgggg ataatatgaa agtcaaggta gctgtaaaat tccttgataa tcagacaaag
      541 gcgacgcagg tattgcagta tgagcttatt ctacataagg atagtaactg gaaaattatt
      601 atgtaagaca ttttgttggc aggatctatt ttcggatata gtctgccttt tctgtagcaa
      661 atgtactgca ctgacaagct atttcattgt attttaagca attaaacctt tcttgacaat
      721 tttatcttaa atcatatatt tatggatatt gactttctgc ttgcaataag ctataatatg
      781 actataaaat caaatcagtc atataggagg tatgtggtat gtataaagaa gttggtattt
      841 cttcaaataa tgttccgata acactttcta tctggtatac agaaagaagt agtcctacca
      901 ttgttttttt gccgggtact atgtcacacc ctctcatgta tgagaatttt ctatgtggtt
      961 tgagtgaaag aggatttaat ataataggca ttcactatct ttcacacgga aaaagcccta
     1021 aaatcaaaaa aaattataca atggaagatt tgctgcataa tgtatatgac gcaacaactt
     1081 acggaataga aaatttcggt gaaaacatag ctgtcatggg ttccagtcaa ggcgggattt
     1141 tagctgcaat ggcggcaggt agggacacaa ggttaaaagc cgtttttcca cataatatca
     1201 tgctgaccac attaaaagaa actatgagct taacaaaata tcccacatgg tcgcaccgtt
     1261 ttatggggct gatacagtgg ggctttcgtg taggtggaaa attgctgcct aactacaagg
     1321 ttccaggaga cgcttattta gactatgaca gagtatttta tagtgaacag gcaaaacaag
     1381 attgcttaaa tgaccctatg cttttaccct attatccttt aagatttgtc accagcttat
     1441 ttaatgctga cttatcgtgc ctggagaatg gggctataca atgtcctgtt attttaatta
     1501 ctgccaaagg cgacccgctt tttagctttg attataccaa caaagtattt gatttaattc
     1561 atgtaccgca aaaggaatta cttgctttag agcttaatcg tcacatgatt tttaatgagg
     1621 acacggacac tacaataaac gcattggaag ctaccttgca taaatatctt ggatagcata
     1681 ttgattatat aatcagtaaa tgttatactt ttcatgacga tataaatatt ttagtcataa
     1741 ggaggttatg agtttgccac gatttacgga acaagaaaaa gaaatcataa acagtaaact
     1801 tttgatagag ggagaaaagc tgtttgcatt acacggctta aaaaaagtaa cagttgatga
     1861 tttagtagct gctgtaaaca tatcaaaagg ttctttctat gctttttatc ccagtaaaga
     1921 acatctatat gttgaaatca attttcgctt acaaaaagag ttatttacaa gtatagaaac
     1981 aaccattaaa aagaaaaagt ataagaacca tagagattta gcaaaggacg ttatcatgct
     2041 aagtttaacg ggggtgatta cttcgcctat tctttcacag attgatttat ctttaatgga
     2101 ctatctgcaa agaaaaatat cgtcagatat ttttgaaaat catatgtaca gtgatattcg
     2161 tatattggag atattggaag atttaggggt tgtatttttg gttccgcata cggttattat
     2221 aaaatccctg tattctgtct tatcctgttt ggaacaattc aaagaggacg aagaactgaa
     2281 tatgatacaa aatcttttag tgaatggtat tattcagcaa gtagtagcag agtaacaatt
     2341 ttgtttggtt atttgacagg caggtagcca aacaaatttt ttacagttta tatgactata
     2401 tatctataaa cgtcataaag ttaaaggagg aatgaataat gattgatgta aaaaaactgt
     2461 attttagcta tactgacaaa ccgtttgtgg agaatgtaag tttccatgtt gggagaggag
     2521 aaatttttgg ttttttagga ccctctggtg ctggaaaatc aactattcaa aaagttttaa
     2581 ctgggcttaa tacaagatat aaaggcagtg taaaagttgc aggtacagaa ataagggagc
     2641 atacaaatca gttttatgaa aatattgggg tagactttga attttcaacc tgctacgaga
     2701 aattcacagc acgacaaaat ctcgcctatt ttgcttctct atatgaaaag caacctcggt
     2761 ctattgatga attattgcat atggtaggct tggaaaatga cggggacaaa aaagttgctg
     2821 acttttccaa aggtatgcgt tctcgcttga atttcattaa agcgttagtc catgaccctg
     2881 atatattatt ccttgatgaa cctacaagcg gtcttgaccc tacaaatagt cgtttaatga
     2941 aagatattat tcttgcggaa aagaaacgcg gaaaaacgat tatcattact actcacaata
     3001 tgtttgacgc aacagagcta tgtgaccaag tagcctttat cgttgccgga aaagtcagtg
     3061 cattggatag cccgcataat ttgattatgt caaggggagc tgccaaaata caatatacct
     3121 actatgataa aggcgagaaa accggagaat gtctgcttga tagaaccaca gaagataagt
     3181 tgttaaaaag tttaatttcc gaaaaccgtt tgctttctat tcatagtagt gagcctacat
     3241 taaatgatat ttttgttgat attacaggga ggacacttca atgagattgg gacgtttaat
     3301 ttgtggtgat atacatttcc aatggaaata tggtttctat tttatctact ttatattaac
     3361 agttttatat gtgtgcggga ttgccgcttt gccggaacat tggaaaacgg atattgcttc
     3421 tattatgatc tactctgacc cggcagcaat gggattattt tttatgggag cgattgtcct
     3481 cttagaaaaa agtcaaaagg tgttaaatgc tatggtggta tcccctgtaa aaatatcgga
     3541 atatatactt tcaaaaacag ttgcgttgat tgctatttct acggtaatcg cattgatatt
     3601 aggggtagtt tccggcagca atcatttact gggtatcgca gttggtacgg ctttgacctc
     3661 agcaatcttt actatgcttg gaattattgc agctacaaaa atttcaaact tgaaccaatt
     3721 tctcattgtg attatgccga tagaaattat ttgctttgta ccacctattg ttggtctgtt
     3781 tgtgaagtta ccatatctgt tccgcttttt tccattcaca gcttgcatga accttataac
     3841 tggaaaaagt gttttactat cgtttgatat ggtacttgta attgctacgt tgattatcct
     3901 ctacatagtt gctcgacata cagttgagca tatgtggaaa agtttgggag gtgtaaagct
     3961 atgaaaaaga tattatccag caccatacag atatttaagc aaatcaaaag tgaccctatg
     4021 atgtttgcgg cttgcttctc cccttttatt atggggactt taattaagtt tggtattcca
     4081 tttcttgaaa gaataacgaa gttttcttta caagcgtatt atccgatttt tgatttatta
     4141 ctttctatca tggctcctgt attgctctgc tttgcatttg caatgattac attggaggaa
     4201 atcgacgata aagtgtcacg gtacttttcg attacccctc ttggtaagtc ggggtatctc
     4261 tttacaaggt tgggagtacc ctcaattatt tcggcggtca tcgcttttat tgtactgtta
     4321 cttttttcat tagaaaagtt gtccattgga atgacaatat gtttggcgct tctcggctca
     4381 gtacaggcaa ttattgtttc acttatgatt atcacattat caagcaataa attagagggt
     4441 atggcagtta caaaactagc tgcacttaca ttgctcggaa taccagcccc cttttttatt
     4501 gatagttact accagttcgc ggttggcttc cttccatcat tttgggtagc gaaagccatg
     4561 cagaatgaag cggttcttta tttctccata ggattggtgg tagctttagt ctggtactac
     4621 ttccttacaa aacgtctgtt tcggaagctg gcaggataag gaggcatatt atggaaaaag
     4681 gtagacttct gcaaaacaaa aatatgaata aggctattca ggtttccaaa attacactaa
     4741 tcatcaatct gagtttatcg ctacttaaat ttgctgccgg atatatagga aagtcaagtg
     4801 ccatgctgtc ggacgctgta cattcggctt ctgatgtagt aagcacaatc gttgtgatgg
     4861 ttggaattaa aatatcagaa aaacagcctg atagagaaca tccttatggg catgaacgta
     4921 tggaatgtgt ggcttccatt attctctctg ttgcattggc tataacgggt gctggaatag
     4981 gatattcagg tatcaagaaa atcttttcag gacagtacaa tacgctttct gtccctagtg
     5041 gaatcgcact tacggcagca gtcttatcta ttgttataaa agagtggatg tactggttta
     5101 caagaagtgc tgcaaagcat acaaattctg acgcacttat ggctgacgca tggcaccacc
     5161 gctctgatgc actctcgtcg gttggttcct taattggcat tttaggagcg cgtttaggct
     5221 atgctattct cgatcctatt gcaagcgttg tgatttgcgg ttgtatctta aaagctgctt
     5281 tgaacatttt caaagaaagc attaacaaaa tggtagacca tagttgtgac aatgctactg
     5341 aaacaaggat tagggaagtt gtgcttcagc agcaaggggt tgatggtatt gatgaattaa
     5401 aaacccgcat gtttggagcg aaaatgtatg tagatattga aatattagct gatggtaatt
     5461 tagctcttta tgacgctcat agaattgctg agggagtcca tcaaacgatt gaaaacaact
     5521 ttccacaatg taagcattgt atggtacatg ttaatacgaa tgaacttctc tattctgcat
     5581 aggacaggga agatgattta aggctattga aagggaaagg agggaagtag gttatggaac
     5641 aatactggtg gattatcatt gttgtggttt tgattgtcgt tggcttactt aaaattgtaa
     5701 ttatgaagaa gtataaccaa aggagaaatt ctaacaatcg ttcaaaagat agcgaggacg
     5761 acaagatgta ttaaggttta ttgaaatctg ttatgttgta gaatgatgtt tttagatact
     5821 agaaagtgct ttaagggggg ctaacgttaa ggaaagcttt gagtacaatt ctttacaatc
     5881 aacagtcttt tgcatagaga aatgtatgta ggaaatatgg tgcaaggcac aaaacaccag
     5941 caattgagaa atcaacctat gaaccaaagg cacactacat tttatactac ttgacaagta
     6001 aacgaacatt tactttaatg gtttaaagaa gtaaacgttc gtttactaaa gaaggaggtt
     6061 gtaaatgtct gacacaaaag aaaatatatt aaaaattgca ctacacttat ttgcagtgaa
     6121 tggttatgag gctgtttctg ttagtatgat tgcggaagcc ttgggaatga caaaaggagc
     6181 attatataaa cattataaaa ataagcaaga tatttttgac tgtatcgtag aacgtatgta
     6241 tcaaattgat aataagcgag ctaaggatta tcaagtacca gaaagcacaa tagaggtaac
     6301 cccaaatgca tacagaaata caactattgg aagtctaata acatttactc ttgcacaata
     6361 cgatttttgg gtgactgatg aatttgcgtc taattttcgc aaaatgttaa tactagagca
     6421 atatagaaat ttaaaaatga atatgcttta tcaaaaatgt cttgtaaatg gtcctatcag
     6481 ttatatagaa gatatattta atgaaatgat tgaaaaaggt gttcttcaag agtgtgatac
     6541 aaaacagctt gctattgaat attatgcgcc attatttcta ttgataagtg tagcagacgc
     6601 agttgaaaat tgttgggatt taaaaactca attaactaca cacgttaaac aatttgtcaa
     6661 gaaatatatt ataaatgaga aagtaaaata atcctttatt ctgtcctaaa tataaaccta
     6721 actttttaat ttcagcagag aaactcaaaa tgaatattgt tataagtcac aaatattaac
     6781 tattaaaata ttacaataac ggagatgaga aaatggaata tattcgcagt aaaaaatatt
     6841 ttacacccga cttacagaca aaaattatgg ggccgaatcc tataaaatta caagaggaac
     6901 tattacaaaa tcataaaatc ccgtctggta gctatgtctg tgaccttgga agtggacaag
     6961 gtttaacttc tgtatttctt gcaaaagaat atggatttgt tgtttttgca gttgacttat
     7021 ggagtgagcc aaaagaaaat gaagaatttt tcaaaaaaat gggattatct tctgcacaaa
     7081 ttaaatctgt aaaagctgac gccaatgaac ttaaatttga gaaagagttt tttgatgctg
     7141 tagtaagtac agattcatat aattattttg gacgtgaccc tatatattta gatgaaaaat
     7201 tgcttccatt tgttaagaaa ggtgggtttg tttatatcac agttcctggc atgaagaaag
     7261 attgccatga gaaacttcca gaagagttat tgttatcatg gacaccagaa gaacttgatt
     7321 atatacatga tataacatat tggtctaaca taattagtca ttgtaaaaag gcagatttaa
     7381 tttcaatata tgaaatggaa agtaattcgg aagtttggtc ggattggata aagcaaaata
     7441 atgaatatgc aataaatgac cgtaaggcaa tagaagctgg cggtggagat tatctgaatt
     7501 ttattgctat aattctacaa aaaaaataaa ttctttagaa ccaaacacta taatttgcgt
     7561 tctaatatgt ttaccgattg acatcaataa ttttatcttt gagggtaatg atttgaaaat
     7621 agaagaatac aagaagatac gaaattgata aattagaatt tgatgggatg gtgatattat
     7681 gaaaatatta tggatcatta ttattatggc agataatctg ctgccgttac tgcttgctcg
     7741 tttctatcca aactataagc ataaagaaat ggcactatct gtccttggct ctcgtcaaag
     7801 cccggtgaaa tggatatata atgtgtggtg cattatatca ggtgtggtat tttgtatcgc
     7861 tccatatgcg ttgtatcaag agaacgatag cggatgggca atcgctatat ggatgcttct
     7921 tgctatatac ggcatcgggt gtgaaatcat ttccggagtg tgcccgttaa atgaaaatag
     7981 acaagacgaa gatataatta caaaaattca cggcggcaca tcggcaatcg gatttatggc
     8041 cttgcttgtc gttcctttgc tctttgcaat tctgcaattc caaacaacaa aagtagtgtt
     8101 aggcgcaatt tcactggtgt gctttacaat agcattcata ttcttctgct tttttattat
     8161 gggtgagaag gaaaagtttg caaatactgt tttgagatat ggcggtcttt ggcaaaggct
     8221 tgttttaata agttgttata taccgttatt tgtttggtct gtttctaatc aataaaattt
     8281 agtttatagg aataacaaat tccagtttgt aaaagtcaat aaatccaaag ataaatttgt
     8341 atttgaaagg agttttgaag aatgaaaaaa tatgagtatg tagaaattaa ctataatgcg
     8401 aaaggagttg tattcctttg tactgataaa cacaaagaaa ttattgacag ttacgcaaaa
     8461 acggggtatc actacgcagg gtttgtaccc acagaaattg acgcaaaagg ctgtatgcga
     8521 aagatagatt taatttttga aaaagaagat taacttattt tagggagttg gttctaatga
     8581 taggacttaa aagacgagat atgaattata ttatgaaagt tggtgctaaa gctcaaggtg
     8641 tttctgtatc tgaatttagg gctatgctac aagataccat agacaaaact atggaaagta
     8701 cagacccaga cgtgcaggca aattttaaac gatactttgg aaataagcgc ccaacaccag
     8761 aggaatatat ttatacgatt accaaaaaga caaaggttaa ggtttaaatg ttgtaaaggg
     8821 ggcttgccgt atgaaacgct ttatgtacaa ttcagtatat tcaagcccaa tttaacagca
     8881 tactatttac aattaaatac gctataccag tagctcgtac aaaatttata ttttgtgcgg
     8941 gcttttttaa tacccgaaaa gtttttttaa ttattttcat ttttttggaa gcaaatcaca
     9001 ccttgctgta cagatatggt gcggaaagag ggataaacac ttttcaattc atagctggaa
     9061 aggaggtgag attatgaaac cgtctgactt ccaaaaaaca attcagtgtc agtttgaatg
     9121 taagctgaaa aaagttgtca aaggtattat ctggaactac cgtaaagaat taaaacgaag
     9181 aaaaaacaag gaaatatctt ttagcgaatt accagacatt acggtagaaa gtctggcaac
     9241 atgggacgac tacgaaacaa actacacaat ttttaatgtg tgtggcatag atattaaagt
     9301 ccttgatgat gaacttgcag aagctctcaa acagctaccc gaaaaaaaac gtaacaatgt
     9361 attgatgtat tacttcttgg aaatgagtga tacagagatt gcagatatta tgaattatac
     9421 cagaggggct gtgttcaaac acagacaccg tgcactagaa actatgaaag agatactaaa
     9481 ggagaagaaa tagaaatgaa gaaaaagtac aagcacccgt cttattattt gatttcatca
     9541 gctatggatg gggatgaaac tgcgattgag aaattactgg cattctatga cccgtacata
     9601 tcaaagtgtt gtttacgtcc tttatatgat gaatacggca atgtttatat tgttgttgat
     9661 atggaattaa agggacgtat tcgagaagcc ttaatcaaaa tgattttaga ctttgagatt
     9721 gatatagata tagaaaccga agaagaataa gcaaatacca gcagggcgtg attggttaaa
     9781 ttcccttctt tcctgccctg ctcctgcttt tatacatgga agcctacggc tttcgccgat
     9841 tgttctttga caactgaata aagcagacag atacgtttgt gatacagcga gccacgggga
     9901 ctgtacgcca tgacctttcc gacaagaaag cgagcgaccc acgcagttga tccgagagcg
     9961 acttgtggaa tggtcgtttt gccatgacct gctatcacag aataatgata cgtccgcatg
    10021 gtgcggttct gcccacaaga atgggaatag ttgagatact aacggagctt tccaaagccg
    10081 tctgtctgct tatgataaga cacacagaaa ggagttgttg caacatgaac aatactgatg
    10141 tgccgatttg ggaaaaatac acccttagta tagaagaagc gtcccgatat tttcgtatcg
    10201 gggaaaagaa gttgcgtaaa cttgccgagg aaaatctgga ctctggttgg gttatcgtta
    10261 atggaaaccg tattcagata aagcgaaaac agtttgaaaa aattattgat acactggacg
    10321 aaatctgatg tcttagagcc gtagatatgg tataatatta gtagcgtatc acggctcatt
    10381 ccatgacgga aaggagcttt acactatgtc aaaaatgaaa agacaagaca gtaaaggacg
    10441 caatttgcgt cttggagaga gccagagaaa agacggaaga tacgtttata aatataccga
    10501 tatattcggg aagccacagt ttgtatatgc ttggaaactt gtaccgacag acaaaacacc
    10561 tactggaaaa cgtgatgata aatcattgag ggaaaaagaa aagcagataa aaaaagacct
    10621 tgacgacggt atcgacacta tcggcggtaa aatgacagtc tgccagcttt atgcaaagaa
    10681 gaacaaccag aaaaagaaca tcaagagaaa cacggaaata ggacgacagt atcttatgaa
    10741 cgcactagaa aatgacccat taggtatgag ggcgattgat acagtcaaac agtccgacgc
    10801 taaagaatgg gctatcagaa tgaatgacaa aggttattcc tataagacaa tagataacta
    10861 taagcgttcc ttaaaggctt cattctatat ggcgatacaa gacgactgta tcagaaaaaa
    10921 tccttttcat ttcaagctaa gtgatgttct ggaagatgat acagagccta aagttatctt
    10981 gacaccagag caggaagaaa aacttctttc ctttatggaa acggataatg tttacagcaa
    11041 gtatcgtgat gaagttattc ttctgctgga aacgggactt cgtatctctg aattatgcgg
    11101 actgacaaca cacattgata tgcttaatcg tgtgattaac atagaccacc agctattacg
    11161 tgatacagag gtgggctact atatttccac acctaagaca aaaaacggca agcgagagct
    11221 tccattgaca gaaagagcat atcaggcact tgaacgaatt ttaaagaacc gaggaaaagc
    11281 tcaaccgctt gttgtagacg gttatagcaa tttcttgttc ttgaaccgtg agggcttgcc
    11341 aaaggtagca ggaaattatg aaagtatggt gcgtgggctg attaagaagt acaacaagac
    11401 ccatgaggac aagttgccga acatcacgcc ccactcattc agacatacct actgtacgaa
    11461 catggcaaat aagggaatga accctaacac cctgcaatac atcatgggac acgcaaacat
    11521 aaccatgaca ttagggtatt atgctcacgg tactttccag tctgcaaagg cagagttgga
    11581 aaggctggct tcttaatatc gaaaatgtat cttactactt atttactact tttggttgca
    11641 ttttcatgct ggtaaatgcc agcttatgcg aggtatcttc caaaaagaaa atgcccatac
    11701 agcccgaaaa tacgggatat atcggcattt accaacttat gaaaagataa tcagaaatta
    11761 gtaagttttt atatcaaaaa aatgattact gtaaagaaat cattttttat tataatcact
    11821 gatacatcaa atattctaaa taaaattttt atttatctgg aattgtatat tttttctata
    11881 atattaacta cagcgtacat tgcccaagca cagatagcca atacaaatac tcccatcatt
    11941 actaaatcta atttaaatac ttgagaacca tatactatca agtagcctat tccatatctt
    12001 gaaactaaaa attctccaac tattacacct acccatgcca taccaatatt aattttagtt
    12061 aaatttatta aatttcctgt gtttgcaggt aatatgagtt taaataatat ttgagattta
    12121 gtagctccaa aactttttaa catcttaatt ttttcttcat ctatattcat aaaataatta
    12181 taagctgaaa gtatagtaac aacaacagaa atcgttacag ctgttacgac gattccttct
    12241 attccagcac ctacccaaac tataattata ggagctaaag cagtcttagg aagagcattt
    12301 aaaactacca aaaatgggtc taatattttt gataattttt ctgaccacca aagagcaatt
    12361 gctaccaaaa tacctaatac agtacctata acaagaccaa gaacagtttc atatacagat
    12421 attccaatat gtttaaataa ttgaccacta aatgcatatt gaataaataa attgtaaata
    12481 tcacttggtt tgctaaatag aaatgtattt ataacattta agtcagctaa aagttcccag
    12541 attacaataa aaccaattaa aattaatagc tggtagaata atacatttct tttttctctt
    12601 tttacacctt taacatagtt tatatatcct tcagatcttt tattaagcct catgtgtatc
    12661 tagctccttc cataatatgt cgaaatactt ttgaaaatca ggaactttac gtgttttgag
    12721 aggcgttttt gttccatcca tgttaagttc aatatcgtaa atatttttta tggtagctgg
    12781 tcttttagat agtattgcaa ccttatctga tatgcttatt gcttctgcta tagctaaaaa
    12841 agtgcaacta attgcttaaa gaaacagaat tatcaacttc agcacccata taaacaatat
    12901 taagatttcc taaactgcta tcccaaacga tactattaat aatagtagat aataacaatc
    12961 tcttatcatc taaactggca ttatcaactt catcattaaa tctcttcaaa ttactaacaa
    13021 taatatcaat attaatactg tcactactaa tattgatgtt actagattcc aaaatattaa
    13081 tcttatcctt taaaatacta atctcattat ttaggctctc aatctgggaa actatataat
    13141 cagaagcagt actaccagta ttttttgcaa gctctataac taaattttta ataagtcttt
    13201 ccttatctaa aatttgattt ttaagtaaat tagtctcaga tttaaaaata ttaaaaacat
    13261 tatcagtctt aatgttatta ctattgaatg cacttataat agactcttct ttacaattct
    13321 taattttatc tataactaaa ctttcaacta atggaccatt aagattttta cagttgcaac
    13381 tatcagaccc aagtgttttt ttagtaccac aaatataata atactttaaa tccttgtcag
    13441 tttttttgcc tttataggta attctcatat tagaaccaca tttaccacat tttataagac
    13501 cagataaaag agcagttttt ccagttgata tccttggagt tttatcacga tttttatcta
    13561 attgattttg tacttcaagc catatttcag gttctatgac tccatggtgt tttgcaattg
    13621 ctgctattga gttagatgta tttttagcat aggtaaggat tccacatttt ctatctggag
    13681 tacctacaac atccatacca ctatttttaa ggtaatctaa aatttcatca ttagctttta
    13741 catatgctgg atttctaagt attctagata aagaactagg gtcaaatttg ccaccacgag
    13801 gaccccttat tccattatta tagacatatt tatataactt agataaggac ctaagttcta
    13861 aatatttatt gtatattagc tttacaatct ccatttgttc atcatttact tgaagcttat
    13921 acatacttct ttcttttaga ttttcatcta agaatgtgat tttttcactg ttgaatccat
    13981 gaggtgtatt gcctccaagc catctaccac tacgggctaa ttcatacata ttgtctttta
    14041 tacgttctgc tattgtttct ctttctaatt gagcaaatac acttgatata aacatcatag
    14101 ctcttcccat tggagttgaa gtatcaaatt gttctttgat agatataaat gatatgctta
    14161 gttcatttaa tttttctatt aaagtagaga agtcagatac atttctcgat atcctatcta
    14221 atctataaca aactaggtaa ctaaatttct tatctttggc atcctgcatc attttcttaa
    14281 attgtggtct atctatattg ctaccactaa atccctcatc ttcatatatc aaaaaatctt
    14341 ttacaccaat agactttgca taatcaatac ataattgtat ttgattttct atagaatcac
    14401 cttttccagt aaatcttgat tttcttgaat aaattgcagc tttcaaaatt aaaaccccct
    14461 ataagttagg atatatattt gaaactattt atctacaacc ttttaagtaa acttattaga
    14521 atgaatttat taattaggta aatttatata attctacttt ttataatata tgactattta
    14581 ttttatttct ttactattca agttctaaat caataataaa ttttgatatt taattgtatc
    14641 aggacaaatc tctaatttca tagattatta atataaaatc ataggttagt gtgaaaaagt
    14701 tgaaatttaa atattttaaa agataatttt tttgaactaa taggtataac aaaaatttat
    14761 agggggattg catatggaaa tcaatgttat agaaattttc cctaaagata aagctaaact
    14821 taataaaata gaaatggata aagctagttg gtttgtaaat ataataagta aaaaatatcc
    14881 taaagaagct ttaaatgaag catttagtac tttagaaaaa gaattaaata taagtaaagc
    14941 taatacataa gctttgatta ttgattttat atactattgg ttttatacgc tatgacttga
    15001 cattatatta atcaaatagt atatttttaa atatagcaca atgaagtgtt aaaagagcaa
    15061 tggagctcca tagataaagt ctatggagct tttttgcgta tttaacttaa ggaggatagg
    15121 actttgaata actttgagat taaaactaga ataaaatttg gagaaggctc actaaaagct
    15181 ttgcaagaat taaaaaacaa aaatgtacta ataataacag acccttttat ggtgaaatca
    15241 aaaacaatag ataaaataac atctaattta ataaactcaa catatcaagt ttttgaagaa
    15301 atagtaccag acccaccaat agaagtagta gtagcaggaa tagaagcatt taaatctata
    15361 aatccagacg taataatagc attaggtggt ggttctgcaa tagatgcagc aaaatctata
    15421 atggactttt ctaaaaaaat attaaaaatt aacgaagtag agtttatagc aataccaaca
    15481 acaagtggaa caggttcaga agtaacatca tttgctgtaa taacagatag tcaaaaaaaa
    15541 tgtaagtatc cattagtgag tgatgattta cttccagata tggcaatact agacccagaa
    15601 cttgtaaaaa ctgtaccaaa ttttataaca gcagatacag ggatggatgt attgacacat
    15661 gctatagaag cttacgtatc tacaaattct aatgactttt cagatgcact agctgaaaaa
    15721 gctataaagt tagtatttaa atacttaata aaagcatata aagatggaaa tgacatagaa
    15781 gctagagaaa aaatgcacaa tgcatcttgt ttagcaggta tggcatttaa ccaagcatca
    15841 cttggcataa atcattctat tgcacatgta ttaggtggaa aattccatat accacatgga
    15901 agaacaaact ctatattact tcctcatgta atagaatata attcatcaat agtaggatat
    15961 actaacacaa cttactctgc tacagctaaa aaatatgcac aaatagcaga attagtagga
    16021 cttgggagtg ctaatataag aatatctgtt agaaatttag taaatgaaat aaagaaacta
    16081 caaaaagaaa tgaatatgcc aactaaattg acacaatgca aattaagttt agaagatata
    16141 actaatgaag aaagagaaat agcaaaactt gctataaaag atacttgtac attaactaat
    16201 cctaaggtac caactgaaga tgatataata aacatattaa agaacattaa ataactacat
    16261 tttattacat gtaaatataa tattatacaa atagggaaaa tgtttttaaa aatataaatt
    16321 atatattgac aaatataaaa cataaaacta taatgaaagt atcacaaagg cgtgatactt
    16381 tagtagaaag caatggagct tcttaggaat attaatatta ttcttatgga gctcctttta
    16441 ttttaatcaa atagcttttt aataaactat attcaaagaa agcagggaat aaatttgact
    16501 gaagaatcta agcaaagagt tattcaagaa tatgttcctg gaaaacaggt tacattagca
    16561 catatcatag ctaaccctaa tgaagatata tataagaagt taggattagt acttgataaa
    16621 aaagatgcaa taggaatact tacaataaca ccaagtgaag cgtcaataat agcagcagat
    16681 gttgctacaa aggcttcaaa tgtatcttta ggttttatag atagatttag tggttcagta
    16741 gtaatatcag gtgatgtaag ttctgtagaa tcagcgttaa atgatgtttt agaagtctta
    16801 ggaaatatgt taaacttctc atctacaaaa atcacaagga cataataata caatgaagaa
    16861 tataatattt atgggaaaga caggtagtgg taaaactacc ttatgtcaaa aactagacca
    16921 gttagaatta aagtataaaa aaactcaatc agtggaactt tataataatg caatagacac
    16981 tcctggtgag tatatggaaa atagaaacct atataatgcc ttaataacaa catcagcaga
    17041 tgctaaaatt atagcaatag tgtatgatgc cacacaagaa gaaaattata tagcaccagg
    17101 ttttgccagt atgttttgca aagacgtaat aggaattata actaaaataa ataaagctac
    17161 tgaaaacgaa gtcaatatag cttatgaaag actatcacta gcaggagcaa gtcaaatctt
    17221 taaaatagac acagttgata atattggact tgaaaaatta tttaaatatc tagaaaaata
    17281 taaaaatata gatgaagttt aaggggaaat atttttttat tcaaaaatat tattaaagta
    17341 tgataaaata aaaaaatgta taaactattt taagaagatt taatctttaa gcgaggtagg
    17401 aacatgggga aaaaaatact attggttgat gatgaaccac tgttaaggat ggatacaaaa
    17461 gatatattag aaagtcatgg ctatgaaata ataggcgaag caagtgatgg atttgaagct
    17521 gttgaaatgt gtaaaaaaca caaacctgat ttagttataa tggatataga gatgccaata
    17581 cttgatggta taaaggcagg caaaataata tgtaaagaaa atatagctgg tggagtactt
    17641 ttattaactt catttgaaga taaagaatac gtagaaaagg ctaaaagtat aggtgcttat
    17701 ggatatttag taaaaacagc aagtgaaaaa tcacttatcc caaccataga aatgtgttta
    17761 aataaggtaa atgaatttga aaaaatgaaa aaagatttag aaaaagtaaa ttctaaattg
    17821 aatgaaagaa aattagtaga aagagcaaaa ggtatactta taagagagtt taagatttct
    17881 gaggatgaag catacactag aataagaaaa ttaagtatgg ataaaagaac tacaatgatg
    17941 gaaattgcca aaatgattat aataggatac gatgactaat acgataaaaa aaatatcaac
    18001 tgcacaaaca tacttaaata ataacgaaat tgaaaaaatt atagaagtat caaaatcctt
    18061 agatttaatg gcttcttttt atgaagctga tgtttttata gatgtactaa gtaaatttga
    18121 tgatgaagca attgttgtgg cacatggtag acctaaagaa aagtcaatgt actgtgaaaa
    18181 tgttataggt aaaaaggctt taattgaaaa tgaaccagga gttataaagt gcttaaaaac
    18241 aggagaaaga gtaagagata ttaaagcttt aacacaggaa tataaactgg tcaaacaaaa
    18301 tattcagcca ataacactag atgacaaagt tttagcagta ctaatagttg aaaaagatat
    18361 aagtagtgaa gttaagaatg agtttaatgc tagtgaagac gaagaaaata aaaatttaca
    18421 tcagtttata aaacttattg gaaataatga ctttttagtt gataatttaa attcagccat
    18481 attaatattt gataaaaatg gaaaattaaa aattaaaaat cgtattgcaa ctgagatata
    18541 caaaaatctt gggtttggat atgatataca agatatgcat tacaatgatt tagatataga
    18601 aaccaacacc tttgaaaata tattaatgga tgaaaattat gatgagacaa atgaagtttc
    18661 aataggtaat atgtttttta aaataaagac catatacatc aaagatgatg aatttaaggt
    18721 ttgcaaaata gtacaagata taagtgattt aaagagaaaa gaagaagaat tagttttgaa
    18781 aacagtggct ataagagaag ctcatcatag agtaaaaaat aatcttcata cagtaatatc
    18841 tataataaaa aaacaaagta gattatctca aaatgatgaa gtaaagcagt gtttagataa
    18901 cataataaat agggtatttg caatactttc gactcattat ttactatcta aagaagttga
    18961 taataatata tctatacgag aaggtatcaa tttattagtg tcaaatattc aaggtggcta
    19021 tttatttagt aaggatatag atatacgtat aactggtgaa gattttaaaa ttagtggaga
    19081 aaaagcaaca gcaatacttt tggttataaa tgaaatacta caaaattgtt atgaccatgc
    19141 attttcggat agagaacatg gtactataca aatattaata aataaagata acgactatag
    19201 aaatattgct attatagatg atggaattgg atttgatagt aataatataa ataaaaatag
    19261 cttagggaca tatataatag atagttatat aacacagatg ttaaaaggaa atatagagag
    19321 aaaaagtagt gagaatggta cagaagtatt aattactata cctacacaaa ttaatattta
    19381 aataataact catctacaaa gtagtagttt taattgagca aagaggctta aggttattaa
    19441 tagattaagt taattgaatg tatttataaa aattataagt atatattcaa tttaacaggt
    19501 atttattata actttaagcc tctttgcttg tttaagacat acttaaattt taggaggtga
    19561 gaaaatgaga gaagagttat taagtgttgg tattgacatt ggaacaagta ctactcagtt
    19621 aattttttct aaattaatag tagaaaatac tgaatcaaat ttttcggtac ctagaataag
    19681 tatagtagac aaagaaatta tttataaaag caaagtttat ttcacccctc ttatttcaaa
    19741 taatgaaata aatggtcaac aaataaaaga tatagttgaa aatgaatata aaaaagcaaa
    19801 tatatctaaa gaagatatac atacaggagc agtaataata actggtgaaa cagctagaaa
    19861 agaaaatgca aatgatgtac ttcatacttt gagtggattt gctggggact tcgttgttgc
    19921 aacagcagga cctgatttag aaagtataat atcaggaaaa ggagctggag ctcatattta
    19981 ttcaaaagaa cattcaacta caattgtaaa tatagacata ggtgggggaa ctagtaacat
    20041 agcctcattt aaaaggggag aagtaaccga tactgggtgt ttggatatag gtggaaggct
    20101 tgtaaagata gataaaataa gtaaaaaaat aacttatata tcacctaaaa tagataaaat
    20161 cataaaagat aataatttaa atcttcaagt aggtacaatg gctactgtag aaaatacaaa
    20221 gccaatagtg gatataatgg ttgacttatt aatggaaagc ataggactaa agaaacagac
    20281 acctttgttt gattttataa taaccaataa atcaataagt atagatgaaa atattaaaaa
    20341 tgtttcattc tctggtggag tagctgatta tatttactat gatggagaaa taaaagatta
    20401 ttttgaatat ggtgacttgg gaattttact tgggcaagca ataaaaaatt gtgaatacct
    20461 taaaaaagta aatgtagtta aatctatgga aacaataaga gccacagtgg ttggagcagg
    20521 ttctcatact actgaagtaa gtggaagtac aataacttac actagcgaaa agtttccact
    20581 taagaacttg ccagttttaa aattatcaat tgatgaagaa acaggtactt caaaacaaat
    20641 cagtgaagct ataaaaaata aacttaattg gtttaaacta gaaaatgatt tacagaaagt
    20701 tgctatagcc atgataggaa agaaaaatcc aaccttttta gatatacaag agtatgctaa
    20761 agggtttgta gatggaatgc aacaaatcat agaaaatgat ttacctttta tagtaattgt
    20821 tgaaaatgat atggcaaaag tgttaggtca agcaatatat gcattattaa attatgaaaa
    20881 agaaattgtt tgtatagaca gtgtcaaggt tgaaaatgga gactatatcg acattggaaa
    20941 acctatagtt gaaggtaaag ttttacctgt ggtaataaaa acattagttt ttaattaata
    21001 gaaggaggta agtaaatgat tttaaataca aaattatttg gacgtactta tcagtttaaa
    21061 ggcttatatg atgtaatggc taaagctaat gaagaaaaat caggagataa attagcaggt
    21121 cttgcagcag aatcagctga agaaagaata gcagcaaaag ttgttttatc acacataaaa
    21181 ttggaggata taagaaataa tcctgcagtg gcatatgaag aagatgaagt aacaagaata
    21241 atacaagatg ctgtaaatga aaaagtttat gaagaaataa aaaattggac agtgtctgaa
    21301 cttagagaat ggctattgga ttcaaatact acaagtcttg atataagaag aatatcaaga
    21361 ggattaactt ctgaaatgat agcagcagtt gcaaaattaa tgtcaaatat ggacttagtt
    21421 tatgcagcta aaaagataaa ggttactgct cactgtaata caactatagg agaacaagga
    21481 acattatcag caagacttca acctaatcat ccaacagacg acccagatgg aatacttgca
    21541 tcacttcttg aaggtttaac ttttggtata ggagatgcag tacttggatt aaatccagtt
    21601 gatgactctg ttgagagtgt tactaaagta ttaaaaagat ttgaagaagt aaaacaaaaa
    21661 tatcaaatac caactcaaac atgtgtattg gcacacgtta ctacacaaat ggaagcaata
    21721 agaaatggtg ctcctgcaga tttaatattc caatctatag caggttctga aaaaggaaat
    21781 gaagcatttg gatttaatgg cgacactata gaagaagcta gacagttagc cttaaaacac
    21841 ggtacagcta caggacctaa cgttatgtat tttgaaactg gacaaggttc agaattatct
    21901 tcagaagcac atcatggaat agaccaagtt actatggaag ctagatgcta tggatttgct
    21961 aaaaagtatg aaccattctt agtaaataca gttgttgggt ttataggacc tgagtactta
    22021 tatgatgcta gagaagttgt aagagcagga cttgaagacc actttatggg taaactacat
    22081 ggcttaccaa tgggtgttga tgtttgttac actaaccata tgaaggctga ccaaaatgat
    22141 atggaaaact tagccatact attaacaaca gcaggatgta cgtattttat gggaatacct
    22201 catggagatg atgttatgct taactatcaa acaacaggat accatgaaac agcatcactt
    22261 agagaaatgt ttggactaac agcaataaaa gaatttgatg cttggatgga aaagatggga
    22321 ttctctaaag atggtaagtt aacagagttg gcgggagacg catctgtatt attaggatag
    22381 gaggtattta atatgaatga gaaggattta aaagcattgg tagaacaatt agttggacaa
    22441 atgcttggag aattagatac taatgttgtg tctgagactg ttaaaaaggc aactgaagtt
    22501 gtagtagaca acaatgcttg tatagatgat ataacagaag ttgatataag aaagcaatta
    22561 ctagtaaaaa atcctaagga tgcagaagct tatttagata tgaaagcaaa aacacctgca
    22621 agattaggta taggaagagc tggtactaga tataaaacag aaacagtttt aagatttaga
    22681 gcagaccatg ctgctgcaca agatgctgta ttctcttatg tagatgaaga atttataaaa
    22741 gaaaataata tgtttgcagt ggaaacttta tgtaaagata aagatgaata cttaacaaga
    22801 ccagatttgg gaagaaaatt ttctccagaa actataaata atataaaatc taaatttggt
    22861 acaaatcaaa aggttttaat attagttgga gatgggctta gctcagctgc aatagaggca
    22921 aatttaaaag actgtgtgcc agctataaaa cagggtctaa aaatgtatgg tatagattca
    22981 agtgaaattt tatttgttaa acattgtaga gttggtgcta tggaccactt aggtgaagaa
    23041 ttaggttgtg aagttatatg tatgttggtt ggagaaagac caggattagt tactgctgaa
    23101 tcaatgtcag catatatagc ttataagcca tatataggaa tggcagaagc aaaaagaaca
    23161 gttatatcaa acattcacaa aggtggaaca actgcagttg aagcaggtgc tcatatagct
    23221 gaacttataa aaactatgtt agataaaaaa gcttcaggaa tagaccttaa ataaagtcga
    23281 gggagtgtag aatatgaaaa atgatgtaat tcgtcctaat atactaggaa ttaaaataat
    23341 ttcaaatata agtccagaaa tggcacaaaa actagaatta aaatcacatc acaaaagctt
    23401 aggtttaata actgcagact gtgatgatgt tacgtataca gctcttgacg aagcaactaa
    23461 agcttcagaa gttgatgttg tatatgcaaa atcaatgtat gcaggtgctg caaatgcttc
    23521 aacaaaactt gctggtgaag tattaggaat aatagcaggt ccaagcccag cagaagtaag
    23581 aagtggtcta aatgcagtag ttgattttct agagtatgga gcaactttta taagtgcaaa
    23641 tgatgatgat tcaatagcat actatgctca ctgcgtatca agaacaggta catacctttc
    23701 agaggtagca ggaataagag aaggtgaagc attagcttat ttagtagcac caccacttga
    23761 agcaatgtat gcactagatg cagcaatgaa agctgcagat gttaagatgt gtgaattatt
    23821 tgcacctcca actgaaacaa actttggtgg agcattactt acaggttctc aatcggcttg
    23881 taaagcagca tgtgatgcat ttgcagaagc agtaaaatca gttgcagaca atccaacagg
    23941 attttaagga tagttagatg gatgctatag gcttaataga agtaatagga tatgttgctg
    24001 caatagaagc ttctgatgct tgtttaaaat ctgcaaatgt taatattgta agagttgata
    24061 aggttggggc aggtattgtt actttaacaa tatgtggaga tgtcggtgct gtaaaagctg
    24121 ctttagaagc gggtgaaata gcagcttcca gagttggtac attaagaaca tcacatatta
    24181 taccgagaat ccataatgaa gtaacaaatg ctttattcaa aactaaagaa cctaaagtct
    24241 gtgaagtttc agaagataaa gaaaaaaact ttgagattac attggaaata gctgataatg
    24301 tatcgaaaaa tttatcagaa aaagaaaatg taattttaga tgtcaatgaa aacagtggag
    24361 atactgaaaa tttatatagt gatatagata aaacaaacaa aaaaattgaa acagttgatg
    24421 atagtttaac agaagctaat gaggaaaaaa tagaaaacac taatgataaa gaagtattgg
    24481 aacaagttaa aatagaaaat tccgattttg atagagatga cctatcaaag aacagtgtaa
    24541 aaaaacttaa agctatggct aaaaaattag attcttcaat aacttataaa gacttaaatg
    24601 ttcttaaaaa agaagaatta ataaacttag ttaaaaagct tagcagaagg gatagatagt
    24661 tatgacactt ttagataaag atttagtatc tatacaagaa gtaagaactc ttgtaagaag
    24721 atctaaagtg gctcaagaga aactttctaa aatgagtcaa gagcaaatag ataaaatagt
    24781 aaaagctgtt gtagatgcag catttaataa tgctactaaa ttagctaaaa tggcaaatga
    24841 agaaactgga tttggaaaat gggaagataa agtaattaaa aatacttttg catctatggg
    24901 agtttatgaa actttaaaga acacaaaaac tattggaata attggtgaag ataaagaagc
    24961 caatacgata gatatagcta caccagttgg agttgttgca ggtcttatac cttcaacaaa
    25021 cccaacatca acagtaatat acaaaactat aatatctcta aaagcaggaa atactatagt
    25081 tttctctcca catccaaatg caaaaaactg catacttgaa acagttaata taataagtga
    25141 ggctgctata aaggctggag ctccagagga ttcaatatta tgtataacta atccaagtat
    25201 agttggaacc aatgaattga tgaaacataa agatacaaac ttaatactag caactggagg
    25261 agaagcaatg gttagagctg cttactcttc aggaacacca gctattggag ttggaccagg
    25321 aaatggacca gcatttatag ataaatcagc aaatgttaaa ttagcaatca aaagaataat
    25381 agattctaaa acttttgata atggtgttat atgtgcatca gaacaatcta tagtaataga
    25441 aaaatctatg gaagaaactg ttttaaaaga gcttaaaaat caaggtgcat atgttttaaa
    25501 tgaagaggaa agtgaaagat tagcaaaatt catattaaga gctaatggta ctatgaatcc
    25561 acaaatagtt ggtaaaagtg ttgaggtaat agcaaattta gcagacttaa caaatgtacc
    25621 taaaaataca aaagttctag ttgcaagaga aaataaagtt ggtcacaatg tagcctattc
    25681 aagagaaaaa cttacaccaa tactagcgtt ctatgttgaa gaaaatgtag aagctattat
    25741 gaacagatgc agagaaatat tgttaaatga aggtgcaggt catacattct caatgcatgt
    25801 aaatgatgaa gaattggtaa aaagatttgc ccttaatatg ccagtatcaa gaatacttgt
    25861 gaatactcct tcagcacttg gaggaatagg tgcatcaaca aaccttgtac cagcactaac
    25921 acttggttgt ggtgcaatag gtggaagttc aacttcaaac aatataggac cacttgattt
    25981 aataaatgtt aaaagagtag cttatggaat caaagaaata gaagaacttg gaagagataa
    26041 atctcaagtt gaaaaatcaa tgtttgactc aatggataaa gaagaattaa taaatcttct
    26101 tgttcaaaaa ctagtcaaag aactagcata actaaaataa acaatgaaac ttatggctat
    26161 cgctataaat ttctaaaaat ataaactata aaaaactata aaattaaatt tttggaggat
    26221 aagattatgg caagtgcaaa cgcattagga atgatagaaa ctaaaggatt agtaggagca
    26281 atagaagcag cagatgcaat ggttaaagct gcaaatgtac aattagtagg aaaagagcaa
    26341 gtaggtggag gattagttac tgtaatggta agaggagatg ttggagctgt taaagctgct
    26401 acagatgcag gagctacagc tgccgagaga gttggagaac taatatcagt tcatgttata
    26461 ccaagaccac actttgaagt agatgctata ttaccaaagg tatcagctga gtaatataag
    26521 aataattata aaatttaaat tttgattgaa aaagaggcta tctcaaaaat gagtcttact
    26581 aataaactca atgtattttg aggtagtctt ttttatacat ctaataggag atgtgataaa
    26641 atgggtgttt taacagaaaa cgaagtaaaa agaaaaattg gaaaaaacaa aaataatata
    26701 actgaatttt atatagaaaa aggtcaaata ataactcctt ctgcaaaatc ttatttagct
    26761 gagagaagta ttgagcttaa atatgttgat aaactttcag atgtaaacaa tgaacctaaa
    26821 gaagttgtaa aagaagtagt taaagagaaa aaatataaat atactactgt atttggcgcc
    26881 aaattggatg aaaaaccaga acatatgact caattaaatg gaaatctttt agtttttaaa
    26941 gaccataaaa gaataatatt gagaggaaaa atagattctc ttgaatcaga aatactaaag
    27001 gtacagatac tttgtcagaa actagatatg ccaaagcttg ttaaggaatt gcaagaagta
    27061 ttagactttg taagaaatat aatgagatgt gaagttttag atgaaaagct aggggacttt
    27121 aaacttttag gattaactcc tcaagaactt agagaacaat ctcactatcc taaaaaatac
    27181 tttggtatag gacatgagtt cccaagttat gaaatgggag aagtagtagt agctataaat
    27241 agtataagaa gtaatacacg agaggttgaa cttgcagcct atgaagcatt taagggtgag
    27301 tatggaaatg ttcacagaga agatttaata aaggcactta atagattatc gtcagtattt
    27361 tggataatga tatataaggt tagaactaat aagtataagt agttggagga cataatggag
    27421 aatctattaa atgaaatagt ggaagaagta ataactagag ttaaaaaaga agcatttata
    27481 gaagttgaag catcaggaag acacgttcac ttaagtagag aagatgtcga taaattattt
    27541 ggagaaggat atactcttac aaaactaaaa gacctatctc aaccaggtca atatgcatgt
    27601 aaggaaagag ttacaataac tggtcctaaa ggaagtataa aaaatgtagt tgtacttgga
    27661 ccatgtagaa atgaaactca ggtagaaata tctttaacag atggttcaac tcttggacta
    27721 aaagctccta taaaacaaag tggagactta gaaggtactt taagtattaa aatatcaaca
    27781 caatatggtg aagttgaact tgataagggg ctaatggttg ctaaaagaca tatacacatg
    27841 actcctaaag atgctgaaaa attcaatgta tgtgataaag agatagttga agctaaggtg
    27901 atgggtcaaa gacctttaac atttgatgat gtagtaataa gagtgagtga cagttttaaa
    27961 acttatatgc atatagacta tgatgaagcc aatgcatgtg gttattcaaa aggaactgtt
    28021 gctaaaataa taaagaaagc ataaatttaa cctactatat aaattacctc tagggaggat
    28081 ttaatatgaa ttatgataac cttgtagatt taataacgga agaaatctat aaaaaaataa
    28141 atagtaatga aataaaaata tctaataaac ctaaagctgt gattgtattt gaacaagata
    28201 acaataaatt taacttatta aaagatggat ttgaaatagt agaatttgat aaaagtataa
    28261 gggaatgtga aatagtgata gtttcaagac tttgtatgag ggggctatcg aatatagcac
    28321 ttggaaattc aacaagtgat gaagaaagat ttatactaaa aatgattatg aatggcaaaa
    28381 aagtatatgt cctagatgaa ggaattgaat acaaaaaata taaggatact gcaccaaaag
    28441 cactatataa gaaatttatg tcatttgaag atgaaatatg taaatttggt gtagaaatta
    28501 taaaagactt aaatgttata actaaaaata agttaaatat aaagaaagaa ctagaaagta
    28561 ctagaggttc taaaaaagaa gatacaaaag ttttagataa agatatttta aatttaacca
    28621 gtgtagaaag tgtaaaaata gataatgaat ttagtttgga tttaaggaat aaaaaactta
    28681 tatcggaagc tgacttaaga aaaccaacta taaatggagt taaaaacata ttagttaata
    28741 aaaaaagtat aataacacca ttagctgtag attttatgag aatacaccat ctcaaactaa
    28801 agaaactgta aggaggtaat tttatggata taggaaaagt tgtaggtaat gtttgggcta
    28861 caagaaaaga tgaaaaatta tgtggccaaa aacttttagt tgtaaaaatt ttaaaaacta
    28921 aagacacata caaggaaggc ttcatggttg cagccgataa tgcaggagca ggaaatggag
    28981 acttagttct tatatccaaa ggttcctcag ctagagagtc gattgaaaaa tctcatgttc
    29041 cagtagatgc aactatagtt ggagtagtcg attcgtttga ggtgtttgat gaataaaagt
    29101 ataggagcta tagagtttaa aagcattgct aaaggtatag aagtatccaa tgaaatgata
    29161 aagaaatctt cagttgatgt attgtactta aaaagtattt gcccaggtaa atttctaata
    29221 atagtaggtg gagaaacttc ttatataaat gaatgtgtag attatggaat aaaacttgga
    29281 gaaggatata ttgtagataa cttcgtaata aatgcaattt cacaagaaat attagatgga
    29341 tttaaaaata agtatcaaaa gctagatagt atagtctcta taggcgttgt tgaaagtagt
    29401 aaaatatgcg ctggaataaa aatgcttgat aagactttaa aatctggcga cttagtatta
    29461 gtaaaacttc aattatcttt tgctataggt ggcaaattag tatatatagt agcaggagat
    29521 ttaagtagtt tagaatatgc attaaaagaa agtgaaaatg tagtaagaga aaaagaagta
    29581 atatataaga ctgtaatacc atcagtagac agtcaaataa taaaaagtct aataaaataa
    29641 tggggggaat tatgagtata aacgaaatta taatctatgt aatggtagtc ttcatggtac
    29701 ttggagctat agataaatgt ataggtaaca aatttggtct tggagaacaa tttgaggaag
    29761 gtataatggc aatggggtca cttgcagttg caatggttgg tgttatatgt ttagcaccag
    29821 ttcttgctga tgtattaaga ccaattgtag taccagtatt taatatactt ggagctgacc
    29881 cagctatgtt tgctggaagt ttattagcta atgatatggg tggtgcgcca cttgcacttg
    29941 aacttgcgaa agaccctaat gctgggttat ttggtggatt aatagtaggg gctatgatgg
    30001 gacctacaat agtatttata ataccagttg cgcttggaat aattgaaaaa gaagaccaaa
    30061 aattcttagc aacaggaata cttgcaggta taataacaat acctattgga gcattcgttg
    30121 gtggattagt tgcaggattc gaggtaatga tggttcttag aaacttagtg ccaataataa
    30181 tatttgcagt agttatagca cttggattat taaaattcga aaatgctatg ataaaaggat
    30241 tcacatactt tggaaaaggt gtagtaatag ttataacttt aggacttgct gctgccatag
    30301 ttgaagcttt aacaggaata gttgtaatac ctggaatgaa accaatcagt gaaggtatag
    30361 aaatagttgg agatatagca atagttttag caggagcatt cccattagtg tttgttataa
    30421 caaaggtatt taataaacca ttaatggctt taggaaaagt tcttggaatg aatgatgtat
    30481 cagcagcagg ccttgttgca agtttagcta actgtatacc aatgtttggt atgatgaaag
    30541 atatggacaa tagaggaaaa ataataaatg tagcattctc tgtatcagca gcattcgtat
    30601 ttggagacca cttaggattt acggcaggat ttaattctaa tatgataaca ccaatgatag
    30661 ttgctaaatt agttggtggt ataacagcag tattgttagc tatggttatc gcaaataaga
    30721 cactaaagaa ggagaatgta taagatggat atatcaaata tagataaaaa tttaattgaa
    30781 acattagtaa gacaaataat agaagaaaaa ataagtggaa ctaaagacac agtagatttt
    30841 gttagaaata aagacattag tggaataact tctataaagt taccaactgt aaaagttagt
    30901 gaatctgata gattagatac agggaatcca agtgatgtag tttacacaaa agatttattt
    30961 acattagagg aaagcccaag attaggttgt ggtatgatgg aaatgaaaga aacaactttt
    31021 gattggactc taaattatga tgaaatagat tatgtaatag atggaacttt agatataata
    31081 atagatggaa gaaaggtttc tgcttcttca ggtgaattaa tattcatacc aaaaggaagt
    31141 aagatacaat tttctgtacc tgattatgct agatttatat atgtaacata tccggcagat
    31201 tgggcatctc aaaattaata aaaatcaagt taagttaata atcaaataaa taatttaaaa
    31261 tacaaactct taaataagca cgcatattta aaaaagctat gaattaaaat tcatagcttt
    31321 ttttataaag aatgttatat tttagacgta ttttttctat atttactggg agatatatta
    31381 tattttttat taaataatct aataaaatgc tctacatttg gataaccaat actattttac
    31441 tgataatctt ttccttatca ctaatcttac attttaaaat attattttta ctcgtaatat
    31501 tatcataaga atttgtttct tatttaaaac taattagaat tataaagttt attgccatgt
    31561 tattaataaa ataaatccag ttaaatcgag tgctagttaa agtaataaga aacttttaca
    31621 atggtttcct ttatcgattg aaataaaagt taatatggta taatatctaa agagagaatc
    31681 ttatcaatta gaatatgcat agaaacaagt ggcttaaaag gagacgttat atggaagaga
    31741 tagtaaagaa ggaacaagca agtatgaatg atattttttt gtatcttaaa gctaaatcaa
    31801 actcattata taaatttgta atattatata atgagtttat caataaacct aaagattatg
    31861 gaactgggga acttataaac atggttgaag tacatatgct aacagcgatt gaagaaaatc
    31921 caggaataag tccaactcag ttagctaaaa tatggaacag aactaaaggt gcaatatctc
    31981 aaactatatc taaattggag aaaaagggtt atataactaa agaaaagaaa gaaggaaatg
    32041 caaaaactat attactatat ccaaccacat caggagtaga gttaagtaag gcacataaac
    32101 tatatgacac catagatgtt acaagaacat ttgaatactt aatcaaggag tgtacattag
    32161 aggagataga tagtttttat aaagtaattg atttgtttat taatttatta gaagaatctc
    32221 cgcaggaaaa taatgattct gaaaaagtgt agctgagatt gaagttgaat aaagagaaat
    32281 agatattata gaagtagtac ttaagtacac attatataaa aatataatag gtataggatt
    32341 aaaaagttta gtggtttaaa gtttaaccac taaacttttt taatgtctat aatctactat
    32401 tatttaattt actaaaaata aaaaaactca ttatattaag atagaaatag aaaaataact
    32461 agaaaaatac tataatatat cagcttaata cagaaaaaag ttaaaaaatt tgagaaaaat
    32521 aaaaatgaaa acgttttata ttgacataga gaaaaagtag tgataccatt taaatatagt
    32581 ttagtagcta aactatattt aaattataat aactatttta gggggatgtt atgaatttaa
    32641 aacaattaag tgaaaaatac gaagaatatg taattcaaat gagaagagaa tttcataagt
    32701 atccagaatt atcttttaaa gaaataagaa caacccaaag aataagggaa gaattacaaa
    32761 aaataggggt cccttttgta tctattgaac caaatatcgt aatagctact atacaaggca
    32821 aaaatgaagg taagacaatt gcaattagag gagatataga tgcgcttcca atgcaagaag
    32881 attcagatgt tgaatacaaa tcagaatatg atggaataat gcattcttgt ggacatgatg
    32941 ctcatggagc tatgttacta ggacttgcac atatgctaaa tgatataaga aaagatttaa
    33001 aaggtagagt attattagtt tttcaagcag ctgaagaagt tggtggtgga cacaaggaga
    33061 taatacagta ctttaaggaa aatggaggac cggatagatt aatagctact catgtttggt
    33121 ccgatattga agctggtaaa atttctgttg aaccaggacc tcgtatggct ggtgggtctg
    33181 gttggagaat agacataaca ggtagaggag gtcatggttc tagaccagat caaagcatag
    33241 atcctataaa gccagcttgt gatatagttt taaaagtatc ttctatacca gttaatcata
    33301 taagtgtact agaacaatgt gttgtacatg catgtgcaat acatggagga actacaatag
    33361 gaaatgttat tccaaacaat gcagaggtaa taggtggata tagatatttt actgaagaat
    33421 caggcaaaaa agtgtttgac attataaagc aaatgagtaa tggtgttgga caagtttatg
    33481 gagtggatgt gaaggttact catactggag gcataggacc tgttatcaat gatgaagaag
    33541 cagttaagat ggctaaagaa atagtttcta atatagatgg attggagtta gattcttatg
    33601 agccaatatg tgcatcagat tgttatggtg aaatattaaa agagtattct ggcttttatt
    33661 gttatctagg ggtaggtaac aaagaaaaag gaataatata tgcacaacac catcctaagt
    33721 acaatataga tgaagatata ttaaagttag gttgcgaatt tatggctaaa tatgcagttg
    33781 aatttttaaa taaaaataat aactaaaata aatctaattt tataaatggg gggatgaaaa
    33841 tgcaaggtgc attaaaggaa aataagaaaa aaagtggtgg aggattttta ggatggattg
    33901 aaaaagttgg gaataaaatg ccacatcctc tagcattatt tacatatatt acactgatag
    33961 tagtagcatt atctgcaata gcaagctttt taggtttttc agcaacaagt cctgctacag
    34021 gaaagttaat aaaagttatg aacttaataa gtgcaaaagg tttagtacta tttatgcaag
    34081 agtttgttaa gaacttccaa aacttccctg tgcttggagt agttttagtt atggcagttg
    34141 ccacaggagt ttgtgaaaag acaggatttt tttcaactgc cataaaaatg agtttatcaa
    34201 aagttaaggg gaatgcggtt gtatttataa tagcttttat aggagtattt gctaatcaag
    34261 ctggtgatgc tgcttttgta ttagtaccaa ctcttgcagg agcaatattt tatggattaa
    34321 atagaaatcc attagctggt atattctgtg gatttgcatc agttggtggt ggatttgcaa
    34381 cagctgtaat tcctggggga tgggacgtta cgctaacgcc aataactgta tcagctgctc
    34441 aaactattca acctgcattt gatatgacat tattagctag ttattatgca ctatttgtat
    34501 ctgcgtttat agtagctaca gtatcaacaa tagtaacggt taaatttata gaacctaaat
    34561 taggtaagta tactggtaaa ccagaaggta tagatgataa tcaaggtttt gaggtcacaa
    34621 aagaacaagc aaaagcagca aagttagcag gttttacagt tttagcttat gtagcgttat
    34681 taattgcttt gtgtatacca gctaatagtt ttttaagaag tccagaaggt tctttaatat
    34741 ttggagcacc acttatgtct tgcttacagt tttttatagt aatactattc ttcttgccag
    34801 ggattgtgta tggaatgagg gttggtaaaa tcaagactgt aaaagactta aatgatatat
    34861 tatgtcaatc aatggcagca atggcaccgt ttatcgtact tgcaatagtt ataggtcaat
    34921 tcttaacgct attctcagtg tctaatttag cacaggtact agctataaaa ggtggagcat
    34981 tactcaactc attgccacta ccaccacaag ctataatact gctattctta gtactagtag
    35041 cgtttataaa tatatttgtt ataagcggaa gtacaaaatg gatgatattt ggaccagtat
    35101 ttgttccaat gttaatgcaa ttaaacatac atccagcatt tactcagttt gtatatagat
    35161 taggagattc tgtaaccaat catttatcac cattaaatgc attctttata atacttttag
    35221 caacagtcca aaaatacgat aaaaatgcag gtatgggaac tatattctca gctatgatac
    35281 cttacactat aggttacttt gtagtacttg cagttttagt agtagtttgg atgacaatag
    35341 gtataccagt aggaataggt ggacaagttt ggttataaaa catatttaat aattttggga
    35401 ggaaaaaaaa tggaaacaac tgaaataaga tacaattttg atgaagtaat agatagaagt
    35461 ggaacaaact gtcttaaata tgatcttaag agtcaattta tgccagaatc tccagaagat
    35521 gcactaccac tatgggtagc agatatggat tttgcatgtg cccctgagat aattaatgca
    35581 atgcatgaaa gaatagacag aaaaatattt ggatatagct ctcaacaaac tgatgattat
    35641 tatgaagctg tatgtggatg gtttaagaga agatttgatt ggaaaataga aaaagagaat
    35701 atattcttta gcccaggggt tgtgcctgcg ttaggatttt tcacacaaat attaacacaa
    35761 aagggtgatg gtatcataat agaaaagcct gtatactatc cttttgaaga taaaataact
    35821 aacaatgaaa gaaaagtagt taatagtcct ttaaagtttg aagatggtca ttattctatg
    35881 gattatgatg gatttgaaga attagctaaa gatgaaaata ataaggtgtt tatattatgt
    35941 agtcctcata atccagttgg tagagtatgg aaaaaagaag agcttagaaa aatagtagac
    36001 atatgtaaga aatatgattt gtggataata tctgatgaaa tacattgtga tattattcgt
    36061 aaaggtgaaa agcatatacc attagaagta gcatgtcctg attataaaga tagaataata
    36121 acttgtacag ctccaagtaa gagttttaac ttagctggta tgcaattatc aaatatagta
    36181 atcaataata aggaattaca agaaaaatgg atgcttgaaa cagattctaa attagctata
    36241 ttcctaccta atccattcgc aatagttgct actaaagttg cttataatga ttgtgaagat
    36301 tggatgaatc aagtaaatga gtatatagat ggtaatatta aatatgtaga agattttgtt
    36361 aatgagtata tgcctaaagt aaagataata aaatctcaag gaacttactt agtttggtta
    36421 gactttagag ggtatggatt agatgcaaaa cagcttgaat atgtaatgct taacaaggcg
    36481 aaggtattgc tagatgaagg atacatattc ggagaagaag gagcaggatt tgaaagaata
    36541 aatgttgctt cacctagaac aattatagaa gagtgtatga atagaataaa atgtgcattt
    36601 gaaggattat aatagtaata tctaaataat atatttgtgt attttaaaaa aagactatgc
    36661 taaaaaaata tttcaaagca tagtctttta aagttttatg atgagtttaa aaaatttcaa
    36721 aacgttacta ttcacttata tgtttatatt agaaataatt ccacaatata ctcattttct
    36781 aaagttgcac ttttatacac cttgcttcac caatatcatg tgtaaccaaa atagtagact
    36841 tattctcatt cttaataata ctataaacat catcacaaac taataatcta gtctgataat
    36901 caagagctga aaaaggttca tctaaaagta gaatatctgg atttacagaa agtgttctaa
    36961 taagcgcaac tctttgtctc atacctccag ataattcttt tggatacata cttctaaaat
    37021 cccataatcc ataagtcttt aaaaggcttt caactctatc taaagatttt ttatcttttt
    37081 tgccttgaat ttccaatcca atagttatat tatccattat atttctccat tcaagcaaat
    37141 tatctttttg aaacatatat ccaatatttc cagtaatctt tatatcacca ctagttggtt
    37201 ttaaaagatt agtaagaata tttaatattg tagatttacc acttccagaa ggaccaagca
    37261 aagttaatat ttctccttct ttcaagttaa aattgacatc ctttaatact tcaagctcgc
    37321 cttccttagt gtagaagttc atatttatat ttttaacttc tactatgttg tcattcatta
    37381 tgccaccccc tataactttt agctcgtata tattatgtta ttaacaatat gacctatttg
    37441 taccattttt cataaatata atataaatat tttgtttatt tacgtatttt cttagtaact
    37501 tgtacgtata aattatagat aaataaatta taaggtaggg gataaatttg agtaacataa
    37561 taacgaattt aatagagtca atatttacta atccaataac tatattgttt ttacttgcaa
    37621 taactgtgta ttctgttttt aaaataatag aaaataacag agtatattca tttgtaagtt
    37681 caaaattaga cgaaatcaat agagactata agacaagtga gttttataaa aaggtaaggg
    37741 atgactacta tacatatagt aaagaaaatc cttatgcaga cgtaaatata acttcattta
    37801 tagaggaagt tgtatctgat ttaaagcata ataatttgcc attgttagaa aaaattagaa
    37861 gtataaaaaa ttcatcttca ataagtatat tacttggggt tttaggtacg tttgttggtc
    37921 tttctacaat gcttttatgt gttgatacta aagacattat aaattcactt ccttcaacta
    37981 ttagtagtat gcaaactgca tttacaacta gtatatttgg agttgtattt tctctaatta
    38041 taggttattt tactaagata aaagattgtg aacatgtttt gatacaaata atgttaaaat
    38101 ctgaaaattt actaacatct gaaataactc attttaagtc agaaagaatg gatttaaaag
    38161 tagaagaggt aaaaaataca ataaaacaaa taagtaagtc tatagaagct attgaaaaat
    38221 ttgataaaat atctaaagat ttaaatgact ttaatgatga atttatcagt ggtatagaag
    38281 ctttaaaaag ccttttagaa ggttctcaaa gttctattaa gacatttgac cagagcgtaa
    38341 gaaaattaga taaacagttt aatattttaa atataaaatt tgtaaagctt tttgataaat
    38401 atgataatca agataatata aataaagaga ttttatttga tataaaagaa tcttcaaaaa
    38461 atatatacaa tgccactgaa agccaattca aaattagaga ttatataaaa aatataaatg
    38521 caggatttgc tctatatgaa agaagtgctc aagatttatt aactaaatta atgacccatg
    38581 aaaataaaat ctctcaaaat caaaaaatcc ttttagatga aaagtttacc ttagatgact
    38641 caataaaaaa tctctcaagt attattgaaa acttttctaa tgacctacaa gttaaactgg
    38701 atatgatgtt tgaaaattct ttagatattc aagataagtt agatgttatg tttaataact
    38761 cttttatgaa tgatgaagtt cctcttgata gtgaagaatt atttaaggat gatgttaata
    38821 atgtatttaa tccttttagt gaagaaattt atgagataga ggataaagaa ataaaggtca
    38881 taggtgagga tgagttaaat gaataataaa tatagaagaa ctgttaacag agaatttgaa
    38941 aagagtagct tttggccaac atttacagat ttattatcta ctgtattgat ggttgtaatt
    39001 cttatcttat ttagctcaga aagtatttca ggttctgttg aacaagattt agctaagaat
    39061 gtcaatgctt ctgttgaaga aacttttaaa aaaagtggaa tacctgtgaa ggtagataaa
    39121 tctaatgggc aagtaacttt tggtgaaaaa accatgtttg atgtagatag tgatgtttta
    39181 aaaccagagg ctaaagaaat gctaaaaatg tttgttccaa agtacataga gactatttat
    39241 aaagattatg gtgactatat atccaaaata gttatagaag gtcatacaga tgatgtggga
    39301 agttatatat acaacttaga tttatcgcaa agaagagcat atagcgttgc taaatttata
    39361 gtgggagatg aaataggtga ctataaatac aaagataaag tgactaagca tattattgca
    39421 ataggaaggt caaaagctga actcatcaaa aatggtgaca atagcgtgaa tagagatgca
    39481 tctagaagag tagaactaaa atacgaaata aatattaatc aaaataaata attaaatttt
    39541 tataaaaaaa aagaaggcag ttggtgtgag ctttaagcct tctttaagga aaaagtttat
    39601 ttatttttgt tgatacttaa catattatag tttttagaag cagtttgcaa tatattaaac
    39661 gtaaatggtc gtttaaaatg cataactgga tactactgat gtaaaatatt tcattttata
    39721 taatataaaa attatacagt atcaattttc tatattattg tactataatc tattatttaa
    39781 aatattatgt aaaaaaatat cataaaaacg catatcatgt tataattact agagtattat
    39841 gaatatggag gcgtagtttg tgatagaaat aagtataaaa aataaaaaaa attctaaaat
    39901 taaaactttg atacttttat tgatagtatc tatatcaatt tatttaactg gttgtactac
    39961 attagataat ggaaataatc aatcaaataa taatgattct ttgtctagta gtatatcaag
    40021 tgataagaat gtaaaaattc attttattga cactggaaat agtgatgcaa tattgattca
    40081 agatggtaaa acttttatat tgatagatgg gggagataat gatgatgagg gattaatggt
    40141 agattaccta aacaatcaag gtgtaaaaga tattaaatat cttatagcta cccattctca
    40201 tgcagaccat ttaggtggac ttgattctgt tgtcaaaaac tttaatatag aaaatgtttt
    40261 tgtatcgaat ggttcagcag aaacaaagag ttatagggac tttataaatg ctttagcaaa
    40321 taagggttta tccccaagtg taccgcttga aaataataag ttctatttag aagattctta
    40381 ttttgaagtt ctaaatacaa atgggggaga tactactaat gaacaatctc tagtccttgt
    40441 atatactaat gggaatgata aagttttatt tacaggtgat gctgaagaag gtaccgaaaa
    40501 agaaatactc cctaagcttg agaaagtaga ccttttaaaa gtagctcatc atggttctcg
    40561 tagtagttca tctcaagagt ttttagataa agtaaatcct gaatatgcgg ttcttttagt
    40621 aggcaagggt aattcatatg gtcatccaca tcaggagaca atgaataaat taaaaaaaat
    40681 gggagtaaaa gtacatagaa gtgatgagtg ttcagatata atatttgaat caacaggtga
    40741 tggagtattt acaagttgta aagatggaag ttataataaa ggcgttagag aagatggaaa
    40801 ctctggaaaa tcaaacagta atacaagtaa aaatgattct tttaaaaata aacaaaatac
    40861 atccaattca gaagaaatag tgtattttac tccaaaaggt aagagttatc actctacaaa
    40921 aaattgtagt gggctttcaa gaagtaaaaa aatattatca ggcacaattg cagaaagtaa
    40981 gaaaaatgac ccatgtgata tatgttatgg aaaataacaa aaaaacaacc aatttatcta
    41041 taaagcttct gaactttaaa tcatttaaaa aataaaataa atgcataaaa aaataagcta
    41101 taatcaatag ataacacatt ttatctatac gaatagctta tttttttaag tattttttaa
    41161 tagctattta ataactctaa atacaccagt tacaatacct acaatttcaa cttctgagtc
    41221 attgagcata attggttcca taaaatcatt ttctggctga agtcttatta aatttccttc
    41281 tttaaaaaac ctcttaactg tagcagattc accatttata agtgcaacta ctatctgaga
    41341 atttaaggct gtattttttt tgtctactac aacgtaatct tcatctaaaa ttcctgcatt
    41401 aatcatactt tcgcctttta ctcttaatac aaaattgtta ctgcccttta ctaaactggc
    41461 agggaatgga atgtattcct ctatgttttg ttgagctaaa atgggttctc cagctgttat
    41521 ttgacctact aaaggaagct ctattatttc ttgattagct ccagaaacat catttacttt
    41581 actacgttct aaaacttcaa tagctcttgg tttagtaggg tcctttctta tgtatcccag
    41641 tttttcaagt ttatttagat gagagtgcac agttgaagta gatcttaacc ctacagcagt
    41701 acatatttct cttacagcag gtggataacc ttttaatatg atttgagact ttataaattc
    41761 caatatcaag acttgctttt cagttagatc taaatacata acatcactca tttccaaaca
    41821 tattttgttt ttatttccat acactttata tttttaatta taacataatc catttatatc
    41881 tgcaaacatt tgttcctgat ttttaatcgt cctcaatcca ttcaaaattc ttgactatat
    41941 cacgttttac atttttctta tgagctgcat aaagctgagt agttgtgaca ttatcatggt
    42001 caagtaagtt ttgaacatca tatatagaaa atcctgtttg gattagagaa gttgcagccg
    42061 ttgctcttaa tttatgggga ctatatccat tgtctctagt agtatcaagt ggaatagatg
    42121 tatatttttt taccagtgtt cttatagcct ttgctgtaat tctcttattt tgaagagaca
    42181 aaaataaagc atctttttct tcatcattta atagttcatc tcttgttctt tcgttttgca
    42241 aataatcttt tattacatgt tcacaagtat gatttatagg cattaaaact tctttcccac
    42301 gttttctata tattttaaat tcacctcttg aaaaattaaa agaggagata ttgagttctc
    42361 taagctcatt taatctaagc ccgtatgtaa caaataatgc tagtatagct ttgtcacgta
    42421 atttagtttt tctccaatat accttttctt tctctgtaag cccttcacct gtttctactg
    42481 attcaagcat cttagcaact tcatctatct caaggcgctt aatagcatct ggctgaggtt
    42541 taggaagttt tattggattg aatccatcag ttatattatt atctatctga gagtttctgt
    42601 ataagaattt aaatagagta gagatggatg atttttttct tgctaaagct ctattgttat
    42661 tttcaaaaat caatgtgttt ttttctgtat ttttgtaata tctactgcaa taatcaccta
    42721 agaataaatt tatatctctt gattttattg tattaaaatc atcttctgta atatctttta
    42781 cacaatctgc atttgttaat tctttggttt caatcaaata tgaacaaaaa aaattaatat
    42841 cttctaaata agcaagtcgt gtagaaactg caacagaacc ttttaaatat ataaaataat
    42901 ccttcataaa tacaggaagt ttattttcca aaagtagaca tttttctatg agttcatttt
    42961 ctttgataac aatattatca ggtttatctg atttattatg tacttttata attttcttat
    43021 cattattcaa aattaaacct cctaaaacta aaatataaat cttttatatt attttttgat
    43081 atttatacca agtgagtata aatatttaat ttaatcattt tttacatttt taagagatta
    43141 gcagtgcttt agatgaaatt atcactaact attccctaaa tccacattta aagactagtt
    43201 gaatttaagt actctttttt atttctcaat atatttatag aaatatttta aaatatattg
    43261 agaaattttt tatgctctac taaaatcttt tttatataat atttttatat gtttctaacg
    43321 aaaccaaata ttagtaaaat atgtatttat ttaactaaag tttttaaatg ggctgactaa
    43381 gcttaaaata taggatatta tgagagttac tattgaaaaa ataaacccta ttgccataaa
    43441 gaaacttata aatatgttat acttaactat taaactggat atattcaagt tatcaaatcc
    43501 aaataagtat gtaattgaac ttattacaaa aataactata actataaagc tcagaccatt
    43561 taagctattt tttaaatcag cactactaag tgacatatgt gtagatatag aatacattaa
    43621 aaacataaat ataaaatact tcaatgaata tacactaggt gttaacaaca ttattcttat
    43681 aaaattggaa atatcaccta aaataatgtg tataaaccca tttatattta aattattaat
    43741 catacttaca tataacttta aatctatatt ttgagtcact ttcatataac tatctggcag
    43801 taatatctta aaaattaaaa taatcattat tgttccacct ataataggtg caattcctat
    43861 aaagaaattt cctatacttt gataaagatt atctttttta tagctatgac ttacatatcc
    43921 tagtatacca tcatttttac tttctattgg tctgaaaaac ttaactgaat ttattttatg
    43981 catgaaaatt aaacacatta tcaaatgact caattcatga acaaacgttc ctatagctcc
    44041 agttacaata attccagttt ttccaaaaga tgaaaatata aatttattat ttagattttc
    44101 tgtaatgcta aaaatcaatc caaatacaat aaatattcct atgaaacaaa aaatatttat
    44161 tacacttatt tttaaaattt cgattatata aatcataagt taagtcctca tgtaaaagta
    44221 tttaactact ctacttcata tccattacag cttaaaattt tacaagcatt ttctatatct
    44281 ttttctttta ctaagatata gtctgtatca taagtagaaa ttgcaaatat gcttattttg
    44341 ttgtctgcta acaaactact taacttagat aaaattccta caagtgaaaa gtctaaagga
    44401 cctagtatct taagtattct ccactccttt tctgacttga catcagaagg aatattatta
    44461 tttaaacata caacacttaa ttcatcatca gttctagtta tagagtaaaa cttctctgta
    44521 tctatccatg ttggtatttt actgtcatta tttaatctac atactgcata ctcttctacc
    44581 aataatttta atttcataat gtatatttcc tccaatctta tttgtttact taagattata
    44641 cattaaaagt tacataaaat aaaacatata aagtaaaaaa ggttcactat agtgtcctat
    44701 ttctatagcc aacctttaaa acttaattaa attctaataa agagatatat tatctaggtt
    44761 caattataag tttaatggct gttttcttat caccttcgat ttctatatca gtaaatgcgg
    44821 gaatgcaaac taaatccatt ccactaggtg caacaaaacc tcttgctatt gcaattgatt
    44881 ttatcgcttg atttagagct ccagctccta tagcttgtat ttctgcaacg cctcgttctc
    44941 ttagtactcc agccaaggct ccagctacag aattaggatt agattttgat gaaactttta
    45001 gtacctccat ttcaacatct ccttgttaat ttgttaaagt atccattacc taaagtacta
    45061 aatcataatt gccatttact aattacaatc atcatttaaa gtatactttt ataaaaatat
    45121 acttctataa tcgttttaag aaaaccttta ttttttttaa ccattttatt atgaaaaaac
    45181 tacaaatttc tttatattct ataagcattt tccaatattt ctaaattttt tatatctttg
    45241 agctataatt tcctctttat ccatatcttt gtaatgattt aatctttcta aaatataagc
    45301 ttttatttct tttgaaacag cttctacatc tttttgtgct cctccaagag gttcttttat
    45361 aatcttatct attattttta aatcatataa atcattactt gtaagcttca taacatcaca
    45421 agcttcttta gctttagatg aatctttaaa tagaatacta gaaagtcctt ctggagaaat
    45481 tacagaataa actgaatgtt ctaacataca tacatcattt cctactccga tacctaaagc
    45541 tcctccacta ccaccttcac caattacaaa agtgattact ggaacagaaa gtttactcat
    45601 ttcaagtaag tttcttgcaa tagcttctcc ttgtcctctt tcttctgctt ctagaccaca
    45661 aaaagctcct gatgtatcaa taaacgttat tataggtcta ttaaattttt cagcttgttt
    45721 cataagtctt aatgcttttc tgtatccttc tggatgaggc attccaaaat ttctttttat
    45781 attttctttt gtatctcttc ctttttggtg tccaactata gtaacattaa actttcctat
    45841 ctgaccaatt cctccaacca tagaagggtc atctttaaaa agcctatctc catgaagttc
    45901 taaaaaatta gaacaaatat gttctatgta ttccaatgtc gtaggtctct ttatatctct
    45961 acttaatgtt accttttcat atgctgatag atgtgaaaaa gcatcttcct taagcttttc
    46021 caatttctca ttaagtgaat ttattttatc acttaaatct atattatttg ccttagaaat
    46081 agttattaat tgtttaatat cattttctaa gacttttatt ttttcactat tattttcaat
    46141 caaaacagtt cctcctttat gccctatttc ttgtgcatag caagaatttg atataatact
    46201 tccttcattt ttcttctatc tactatcatg tcaataaaac ctttttcaag taaaaactca
    46261 gaagtttgaa atccttcagg aagtttttga tttattgttt gctctattac tcttggacct
    46321 gcaaaaccta tcaacgtatt tggctcagaa attataatat caccaagcat agcaaaacta
    46381 gcagttactc ctccagtagt agggtcagtt agaactgata tatatggcaa taatttttct
    46441 tcaagcttag aaagtgcttg agaagtcttt gccatctgca taagagatac cataccttct
    46501 tgcattctag ctcctcctga agcagaacaa ataattatag gtaaattatt ttcagcagca
    46561 tactctattg aataagttat tttttcccca acaatagaac ccatacttcc catcataaaa
    46621 tcaggattca ttacacaaat aactgtttta attccattta ttcttccata acctgtgaca
    46681 acagcttctg actcttttgt tttttcctta taagagtcta ctttttcttt gtatttagga
    46741 aaatttagtg gattttgaaa ttctacatca gaattaaact ccataaaagt tcctttatca
    46801 atcaataaat ttattctttt tcttgctctt aagccataat gtcttgaaca ttttggacat
    46861 acaaatgaat tttcttctat gtcctttcta aatacatgac tatcacaacc tttgcaatag
    46921 gtccaaaatt tatcatctac actattcttt ttaaagttgt catctaaaga aatcgtaaca
    46981 tacttagatt cttttgatga taaaaatttt tttatcataa tctatttact ccttataatg
    47041 tagcaaattc tttcttcata aagacatctt ctataaattt ggtatgatga gtatcttctc
    47101 taaactcctc acttgttaca agtgcctttt gaaattctac atttgtatta ataccttcta
    47161 caataatttc gtctagagct ctatatattc tacttataca ttcttctcta gtttcagccc
    47221 aacatattaa cttacctatc atagaatcat ataaaggtgg tatagtatat cctgcatata
    47281 caaaagtatc aaatctaaca ccaaaacctc caggtaaatt taatgattct atcttacctg
    47341 gtgatggagc aaaattcttt gatgaatctt ctgcatttat tctacattca atagcatgac
    47401 ctctaaaagt aatatcttct tgtttaaaag gaattttatc cccattagct atagatattt
    47461 gaagttttat aaggtcaaga tttgttacca tctctgtaac tggatgttca acttgtattc
    47521 ttgtattcat ctctataaaa taaaagtttt tatctttgtc aactataaac tcaatagtac
    47581 ctgcaccgat ataacctact ccttttacta tatctactgc aattttatga agtttttgtc
    47641 tttcttcatc tgaaaggtat ggacttaaag attcttctat tactttttga tttcgccttt
    47701 gcatagaaca atctctatca cctaaatgta ttgcatttga atgtttatct ccaaatactt
    47761 gtacttctat atgtctagga ttttctataa acttttccat atataaatca ctattagaaa
    47821 atgctgctag agcttctgaa cttgccatat caaaatttga aaaaagttct tcttcttttc
    47881 taacgattct catacctttt ccaccgcctc cacttgaggc ttttatcata acaggatatc
    47941 caatttctct tgcaacttct aaagcttctt ccttagtttt caaaacagat tctgaaccag
    48001 gaacaacagg gatattcaat tccatcatag tttctcttgc tctagattta tctcccatca
    48061 aaatcatttg ttctcttgtt ggacctataa aaataatatt attatcttca cattgctttg
    48121 caaattcagg attctctgat aaaaatccaa agcctggatg aatagcatca cactttaatt
    48181 taagagcaag atagattata ttttctatat tattataact cttacttata ttatttggtc
    48241 ctatacaaat agattcatca gctatatatc tatgaaaaca atctttatct atttcagaat
    48301 agatagcaac agtttttata cctagttctt tacatgttct gatgattcta actgctatat
    48361 cgcctctatt tgcaactagt atttttttta tcaacttaaa tacctcctac tacaatggtt
    48421 ttatcttaaa taaaggttga ttatattcta ctagttcttc attactaact aatacttcaa
    48481 ttatttcacc tttaatttca cttgttatct cattcataag cttcattgct tctaatatac
    48541 ataaagtgtc gccttcttca actacatcgc caactttaac aaagctatct gcatctggac
    48601 ttggagaatc atagaatgtt cccatcaaag gagctactac tactgataga ttctcattag
    48661 atgtgacagg tgtatcgttt acaactggct tttctaaaga aagatccact actgattctt
    48721 gttgtacact taaaacaggt gaacttgtag attgagcttt tttagatact tctaatctta
    48781 aatcgctact ttcaagtttt acatattcta agttagttga gtctatggcc tttaataatt
    48841 ccttaatttc attgatgttc attttgttac ccccttgtat tattacctgg tgctaatatt
    48901 atatcacaaa aaacaaattt ataaaagaaa aagtgctgac tagagaatca acacatcaat
    48961 atatctttta attttaaatt aacaaccagt aacttatatt tgttttaatt ttccattatt
    49021 aatataaatt ataagtataa aatgaataaa attcaatatt ttttgcaaaa tacaagagaa
    49081 tacaaattaa tacctcggga atttttaaga caaataccaa tattatgcaa tttagacttc
    49141 gctttctttc aaattagtat cattttcttt atttttacat aggatgtact ttaatttagc
    49201 tatcaaaaca ttgctatata ggttaaaagt tattagaatt gcaggaatta taaagtaaac
    49261 tctagcacct ataaaaccaa aaactactgt aataagtatt aatcttttat ttaaagtaaa
    49321 aaaaggtata ttaaaaatac ttttgtcgga tgtttctgga gtataagaat atataactcc
    49381 aaaaatcaaa gtaagcatac aaagtggaag tagtgtataa cttgaaatta ttcccagaaa
    49441 tcctatactt tcaaaatatt tgctagcact atttgtataa acaaacatgt atccatttaa
    49501 atatgcgcat atacatgata taatgccaaa agttccccct aaggattgag tgtaccaatt
    49561 aaaaaattta gattccatat acctcacctc ctaaaactgt atatataatg atatgcattt
    49621 aaatagtaaa atatatcttt atatgtttct gaataaaatt ttgttaattt cattaattta
    49681 tgtttaagaa atttgaagta tagtgtagta tatattaatg tagatgaagt tttatagtac
    49741 ttatttcata gtgcttaatc aagttatctt attatggatt caaaagttgt ttgtagggtt
    49801 ttatattttg ttgagctgaa aaatttatta taaatgaagg gatgttttcg ctttggtaga
    49861 tacattagaa tttggtctta aaattttatt ttttatatta tcaataattt ggatgggtaa
    49921 aataatgatt ttaagaactg ataagcaaat agtaatcaat cctttgctta ttggtatatc
    49981 tgcagtcctt gtgatgttgc atacctcaca gagtaacatt gaattttttg gattagatgt
    50041 ccaatatata agaatagttt tgtatataat atattcactg ataattttaa taggaatatg
    50101 ggctactaac agaagaaatg gaatttttta gataatctat ttctataaaa tttaaaggtt
    50161 gctagtaaca aagcactaat attagcgttc tgttactaac aacctttatt ttgattatac
    50221 acttaaattg aaaatagaat gattgaataa gttgacagat tattttacat tgacagtaat
    50281 tagaacttct tgagatgtat ttaaatcatt tttgtcgctt agtataactt ttaccactgc
    50341 ttgacctgtt ttattacctt taattatcat atcttcacat gaaagtacac tcttatcact
    50401 tacagataca ataaaattta aatcttttgg aaggttatca gtaacttccc agtatttata
    50461 ataactatca attttcttcg taattatttt tctcaaatca atactttcac cttttgctat
    50521 atcaacgtta tgatatttag agtccagttt atactgatta tcttttgtat caataaaatt
    50581 taaatcgcaa tttacatcca caccattatg agaaagagaa tttactacat cgtcatcaat
    50641 caaataatta ttggcaagat ttattgaatc catatctttt agacttaaag cttccttagg
    50701 aatctctttt atattattat tccaaaaaat aaaattgtct acattttcaa gatatgatat
    50761 accttttaaa tttgaaatgc tctcaccagt tgcataaaat tctccatcta aagcctttat
    50821 attgcttata gtcatctcat ctggatttat atatcttttt acaactgctc ttaaatttgc
    50881 atcaggaaaa atatctttaa gttgattttc gctcaaaacc ttatcatttg aatttattgg
    50941 acttaaagct gctgcagaaa taataaatgg tattaaatta gctaaattca tagtaccctc
    51001 ccacataaat tttttattat aataatttaa tttctatgag agggttctgt ttccttgtta
    51061 tatataccta ctttcgtaca ctttattaat ataaattaaa ataaatttct actattaaac
    51121 ttataaacat attactagtt acgtttttta gcttcacatc taaatattcg cataccttgt
    51181 gacataaatt tgtcttcata ctctgttgta acattatttt catactcact atttcctaaa
    51241 tcaagagaaa tattctttaa tagccaatta tttgcagcaa tttcatttaa actaaactcg
    51301 aataattttt cattatctgt tttaaagtga atttcgccat catcttccaa aaccctattg
    51361 tacatttcta aaaatccaga atgagtgagt cttcttttac tccatctatt tttaggccat
    51421 gggtcacaga aatttatata aattctcgat aattctttag cttcaaagta atccaaaata
    51481 ttgcttacat ctccccataa aaatagtata ttattcaaat tagaagcatc tgctttttca
    51541 acagccttta aaagaacttc ttccttaagt tccatggcaa tataatttat gtcagggttc
    51601 tgcttagcta aagttgttat aaacttacct tttccagtcc caaattcaac atgtattgga
    51661 ttatcatttc taaactttaa attccacttg ccttttaatt tgtctatatc gtctcttaaa
    51721 acatattttg tataacttaa aagcttttca tctgcacctt tttttcgtct tcttctcaca
    51781 ataattctcc ttacatataa aaaattagta aaatacattt ttattataca ttatctcacc
    51841 aaaaaataaa aagaactctc ttaaaataga aatttatttc tataaagaga gttctaaact
    51901 tactaaaaac ttaatcttag tggcaccctg aacaaccaga gcatcctcct ccaccttgat
    51961 ctttcatatt ttcaggaata actctaaatc caaatcctgt atcttctatt attatatctc
    52021 catactgact atattcatca gagctcatta caaattgtaa ttctccaact tcataagtta
    52081 agtcatcttc ttttttctca tctaaagcta atccaaaaga aggacctcca caaccaacac
    52141 ctgcaaagta tactcttata ttattaggtt tatcttgatt gtcttttaag atacttttta
    52201 aagtatcaat agctgtttct ggtaaagtaa ttttcatcaa atcattccct ttcaaatatt
    52261 ttattaaaac aatatctatt atatacccat acaaggtaca taatcaatta gtttttgcat
    52321 attttactaa aacttctacc taatattacc taaggcttta aaatatttta tataatattt
    52381 tattacctta aataaatacc ctatcataaa cttagtaaac aaataaaatt attattctaa
    52441 aaaatataat aattttattt gttgttttaa tattataaca catttgataa tgtccaattc
    52501 atatatgcaa aatttatata ctttaaaata tctaagaaat tattcaataa aaaaatacta
    52561 ttttttaaaa attatcatta ttgataaagc tctactattt atatttttta gtgtattcat
    52621 gtataataag taaaagcaca acggtatata ctgttgtgct tttttgtaat taataaacaa
    52681 tattttggtc tataacaatg aaaaatggct actttccata attgatttca tctgacataa
    52741 gcaaaaaggg tgtccagctg ggtcaatcat aactctccag tcatcagtaa attgctcctc
    52801 tgcgattgta gctccacaat gaattgcata ttgaactgct tcttctaaat cattaacagc
    52861 gaaatccata tgagccattt gctgttgaga ttcaggtttt tctggttcta ttatgcccca
    52921 agcctttgtt ttttgcaata taaagaaaaa attatatatt ttaatatttt ataattgttg
    52981 aaattgtgta ataaattcaa ctggatacat agtgcgtttt gaaatttctt ctaaagctaa
    53041 accttgtttt aaaagcctct ttatgacgct ttccatactt tctgaaattt ctataatatt
    53101 tttatcaaaa tcataaaacc taaaagttct ttgaccccat gggtattctt ttatataatg
    53161 caaaaattca atatttccta ttctatttat cttactttcc cattgtttga tgtcattaac
    53221 ctcaaaatac aactgaaagt tatttgattg ttgatatatt ggtaaatcat atccaattat
    53281 agctttataa ttactttgta atccaaatcc attttcaaat gatacatgtt ctcctaaatc
    53341 taatgtgatt ttttgctcca taacatcttc ataaaacttt tttgatttat ttatgtcttt
    53401 gacaattagc aatactcctc caaatttcat cttatcacta tcctttcttt gttataagta
    53461 ttataactta aaattttgca atttcactgt aaaaatcaga catattagct cggtattttt
    53521 gaggagttat tccacagaca attttaaaat ctttaataaa atgtggtata tcatagaatc
    53581 ctaattcatt acatatagac atcaatgaag ttttattatt gtttagaagt tttatagatt
    53641 tattaatacg taccaaacga gaaaatgact tcatactcaa ccctaggtac tgattaaaga
    53701 gtctgtttaa atgcctagaa ctataaaata cttttttaga aatttcttgt gaagatatat
    53761 ttccagagtt ttgaataatc aactgaattg ccaaaataaa ctcatatgag tattcaattt
    53821 taatattaga aatcaataat tcttccaact ttaaaatcaa tttatctata gataaagatt
    53881 cattaaatat ttttttaatt tctaaatcta atgaagtatt aattatagaa aatggaacaa
    53941 ctttatcaat aagttcactt tgctgtatac ctataagcgg gaaaaaacca gcaggttgaa
    54001 attctataat tagtataaca tcacatctat tagcaatatt tcctactgtt tttggtttag
    54061 tagttggacc aaataataga ctatgtaacc ctgtggcatc ataaaaaaac accaaagtta
    54121 cacttccatg aggaataatc gtgtaattat cagaaattat tgtttgattt ggaaacgata
    54181 tagtaaaatt cgatatccaa tttcttaatt gtatacaagg caagagataa acaaaattgt
    54241 cttcacaaac tataagtgac ctatttttat ttataaaatg aatactttct ttttttaaca
    54301 tatattatat cctgtcctat cgtgaatatt tattaaagta agaagaatcc atattatctc
    54361 tatatatcat taactgcttt taataataga gatagtatga atactcatta gatatttaat
    54421 aaacaaataa aattaaaata atcaataaaa aatatagtac ttttatctgt tattttaata
    54481 ttatagcaca tttatagtat ttaacttata tccactttac atatgtctaa tttatataat
    54541 taaaaatgct tatctcaaat acaataatta gtccttatac aattaaacca tctgaaactg
    54601 taatagtacg actactactt ttggctgctt ctggtgagtg agttaccata attatagttt
    54661 gtccattgtc tctgtttatt ttattcagta aatctattat ttctatccct gttttactat
    54721 ctaaatttcc agtaggctca tcagcaaata gaatctctgg ttttccaatt aaagctcgag
    54781 ctatagcaac acgttgttgc tgaccaccag ataactctcg tggtgtatgt ttacgtctat
    54841 cagttaaacc aactatatct aagatttcat ctaattgatt tttataatct tttaaatttt
    54901 taccatctaa taacaaagga agcattatat tttcctccac atttaaattt ggtattaagt
    54961 tataaaattg aaatacgaat cctatatttc tacgacgtat tatgctcatt ttctcatcat
    55021 taaaatgtga tatatcagct ccattcatat acacttttcc gctcgttgga gtatctaaac
    55081 cacctaaaat atataaaaga gtacttttgc cagaaccaga ttgacccatg atagaaacaa
    55141 attcaccttt cattacttgt aaagatacct cttttaacac tcgagttgtt gtatttccaa
    55201 ttttatattc tttgataata ttttttgctt ctacagctaa actattatgt gtaattttca
    55261 tattaacctc cataattatt caaacttaat ttcttctatc agtttcattt tcttcccttt
    55321 tataataggc actatcgaac ctattaaagt tactataatt cccaataacc caacaataat
    55381 aaatgtttta aaatctaaat ctggtttcat tgaaattttt ggtcctgcaa ctataaagat
    55441 agtttgaatc tcaagtatag atatcacaat accaataaca gctccaagta aacctgaagt
    55501 aaacccttca atcaatgtca cttttatatt ttgtttatta cttagtccta tagatttata
    55561 catagctata gaacgtcttt tttgaatata attaatcagt agattattaa taatacctac
    55621 tgtagcaaga agaaaaatga aataggtcat cttattcata ggagacaaaa aactacttat
    55681 agtatttaaa gaatcattat taaattcttc aactgtacga ctccaattat atgtatctcc
    55741 aaataaatat cttatctgaa tcataacagc atctggattg actgctgtat atactaaaaa
    55801 accataatta gttttatcaa aatcattaac tgcataatgt gatggtataa cagcttctac
    55861 atcatttgcg cgggatttaa aactacctac tattttataa gaaaaatcat acttatcatt
    55921 agatagagtt attgtatctc ctatagataa gcctactttt tttagagtat cttcattcaa
    55981 aataacagaa cgtttatcat taaaagcttc tattacttgt ttttttatct caaaatttgt
    56041 ataattgatt ccaaacatag agttatatag ttttatatta tctgtaccct ctagtcttga
    56101 taatgttaca ccattaccag aaatctcatt attcataaca taaagaggta atattttttt
    56161 aattccatcc atatgtctaa catcttcaat aaactcttca ttcatctttc catctgcaaa
    56221 accttgtaat tctgcgtcac gaaacacatc tgttatataa gttttgacaa agttaccaac
    56281 aacacttatt gcaataactg cagaaatact tataaatagt aaagtgatat tctgtattat
    56341 atttttgtta tttttcatat ttctagcagc taatttaccc tcattcccta aaatattttt
    56401 ataaacaaac tcaaaaacta tagacatgat atcagtaata agaggaatca acacaatagt
    56461 agctacaatt aatcccagta atgaaaatcc accagctaga tacaatgtat tttcagggga
    56521 tattcttgga agtaaaatag ataaaataaa cattatactt cctataaaaa gtatagaacg
    56581 atttgaaaca ttcttttctt ctactgttcc tagtacaata ttttttatcg gcaaatggct
    56641 agctttttta acaggtatat atgcactaaa tgaagaaacg attattgcta ctataacaga
    56701 tataatcact ccaatgggtg atattactac tggaattgat attccttgtt cgagagaatt
    56761 tcctaaccca tgaagcatta aatttaaaac aactacacca ataggaatag caattaatcc
    56821 tcctatactt ccgtataata tactttcaag catcaatata cgagttacag ttttttcatt
    56881 ggcaccaata cttctaaatg ttccaataaa aggaagacgt tctaatgtaa taaccttata
    56941 actgctgtat attataaata tactaagggt taatgcaaaa aaacttatta aaaagaaagg
    57001 cattgtcttt tgtctagcat ctgaaattat cttagtttca tttatagtat ttgataccat
    57061 atatttttca gtagaaagtt ctttggataa tttatttact agattttctg taatttcttc
    57121 ttctgactct ataagaactc ttgtatatcc actgccttta tttataattt ttgatagagt
    57181 ctctttgggt agcagtgcat ttacacctct tgtgttcctc aaaaatactg tatcataaga
    57241 tgcaatatca ctaacttgaa aagtataaga ctttccataa atctgcaaag taatagaatc
    57301 accttttttt attttatatt ttgatgtaaa gcgatttgga agaataattt tatcaccaga
    57361 aaaatcagta atactatcgc cattttctaa tcgtggcttg ttgattttat ttagttgact
    57421 taagtctgct gatataagac taaattcttc atagtatccc cctttattat aaatagcaga
    57481 gctttccaat acaccaacct ttgattttat agcatttaaa tcaggtatgt cttctaaatt
    57541 ggttaatata tctggatttt tactttggac agatatagtc gctgtaccat acataccttt
    57601 tgccattttt ctttgtgcac tttcataaga taatccaatt gagaatgaaa caaaaagtaa
    57661 aactgttgat aaaactatgg acaataacat aactgctgtt ctaatttttc gttctttaat
    57721 atttgtgaaa atatacttta atataatttt caaatttaca ccttctttcc ttatctaaga
    57781 tatttcaatc taatattttt atactttaat ctaaattaaa tctctaatag actcctccat
    57841 acttttatca ttctatttat aatattttta atcaaatcac cccttaaata aaaaagattg
    57901 ataaactact atataaatat aataatctat caaccttaat atatttttta tttgacctta
    57961 attaacctta attaaatagg aaaggtgata taaaatgagc tacctaatcc aggtatactt
    58021 tcaacagata tattccctcc atgaacttca ataatttgct tcgttattgc tagtcctaaa
    58081 ccagttcctt ctattttttg ttttgtattg cttcctctat aataacgatt aaacaaattt
    58141 gatgcttctt ctcttgacat cccttttcca ttatcagata taatgatttc aattctttca
    58201 tttaatgtta tatgtacaca aactttagta tttttatctc catatgtaaa agcattgaca
    58261 ataagattac taaaagctcg ttttaatagt gtacaatcaa aatcaaagtt aatattatct
    58321 atattactct taaattcaac tatatttgat tcatattctg gatagtttaa aatatcaatg
    58381 gataattctt tcaaaaatct tactaaatca taattatttt tatttaaagg gattattcca
    58441 ttttttaatt gataggttaa ttttaaatcg tttataagtg tatttgtata ttctatattc
    58501 tttaacatga ttgaagcata tttatatact ttttcaggtg aataattagt actactatct
    58561 gtaagaagct ctgcataccc ttttattggt gaaagtggag tttttaaatc atgagttata
    58621 tttgaaatcc actcttctct cataatatca gttttatttt gtatctcatc acttctatga
    58681 atttcttcat ttaaaatatt taaactatta tatatatcag caaaaacacc tttaatatta
    58741 actggaagat agcttcgttt tgaaatatcg ctaacagcag atgtaatatt tgatatgcac
    58801 tttgttaccc agtaaccata agcaattcca gaaaccaaaa ttattaaaaa tataatccct
    58861 atgattgaaa atacaattga cttaccacct ataaatttac ctccatcaaa aaacatattc
    58921 actttagata tatttaatgg aaaatatata atataaatat agtcattttt attattttta
    58981 attgtattaa agtatacagt tccattactt gaattatttt ggttagctaa tgctaataat
    59041 tcagaattag aataataatc tttatgctct tttggttcta aaaacccata aactctattt
    59101 ccactagaat ctaaaatttg caaccacaat tgattttctc gaagtttttc taatccagac
    59161 tgttttactt gtggcttatt atctataaat acgatttgtt cattaaagtt ttctgtaaat
    59221 tttttcggcc agtcactacg aataaattcc ccatttggtt tctgaatagt aataagtgat
    59281 aagaaaatac ctattactgt caggattaag caaataaaaa tcatgaaaaa aataatgtat
    59341 gaatgaaata ctgttttata actaggttta tgcatttaat caactctttt cttaaacttt
    59401 tattttgatt attgcttata taaaatagac aataattatt gatatgtaaa aatttattta
    59461 aaaatacaac tatctacata aattatattt atataattat atgttatcaa gctatctgtt
    59521 gtatttaata aatccttttg ataacatata aaagtaagat aaatttttcc attaaatacc
    59581 ttataattat acacaaaatc tctatatacg ttaaacagta tagagaaaat tttcatagaa
    59641 ccatctttat aggggatact ttataaaaga ataaaaaaat tataatatat aaaaacttca
    59701 taggagagta acaacatcag taaaaatatg aaaacactat ataaaaattc aaaaatatta
    59761 agaataatga aatacttgaa attgcttata tcaagaaatt ttatataata taaactggtc
    59821 aaatatgtta taatattaaa ttgtgacaag ttcatggagg tgtttatatg aaaaaacaaa
    59881 agtcgccaat tttacttgaa atgcttggac tattttttgg ttgtatatca atgagtattg
    59941 gtataaatat gtttttaaaa ccacatacaa ttgctcctgg aggacttagt ggtctatcat
    60001 tagtactaaa caaggttaca ggacttccag tatcagctat catgttaatt ataggtgtcc
    60061 cattagtaat acttgctttt agaattatgg gtacaaaaaa ttcattaaaa actttatttg
    60121 gaacagttgt attttcagct atagttcaat taacagaccc attatcaaaa ctaagattta
    60181 ctaatgactt attattgtct tctattgcag gaggaatcct agttggtata ggtttaggaa
    60241 taatgtttaa atcagatgct tctactggag gaacagactt aattgcactc atacttagta
    60301 agaaattccc aggaataaaa gctacaaaat tcatgtcatg cctagatgga atggttgtta
    60361 tatcttctgg tatagtaaat cgtagtttag aaactgctct ttactctggt atagcacttt
    60421 gtgtattaat aaaaatagca gatatgataa tggaaggatt tgaccattca aaagcatttt
    60481 ttattatctc tgatgagcct gaaagtttaa gacaagctat aactgaagaa cttgatagag
    60541 gtcttactat tcttgatggt aaaggtggat acactagaga aaataaagaa gttctattag
    60601 tcgtagtttc taaaaaacaa gaactttatc taaagagatt agttaaaaga actgacccta
    60661 ctgcatttgt tattgttaca gatgtacatg aggttcttgg agaaggattt aaaaatttag
    60721 agaactaata aaaattaata ttttaagtat atatactatt ttaattaaaa tacgatttat
    60781 tcaaaaagag tgtctcataa actttttagt tcatgagaca ctcttttttg tataaaatca
    60841 aatgcatttt cagtatttta acaataaaaa caggattatc tatattttga ggcaaccact
    60901 ttattgaaat tttatttaga aaacaatcat tactaaataa ccagcataga tattaaatga
    60961 ttatatctat tgttttttca attcatattg ataacaagga acatttttca cagtatttcc
    61021 agtatgaatt tttttgatat actttcctgt acatttaaat cctaataaat ctaatgattt
    61081 tgtagaattt tggttttctt cataggttat tgcaattatt ttatttaagt ttagttctct
    61141 aaatccatac tgtattactc tttttgttat ctcagtagca aaatcatgtc cccaatagct
    61201 tggatttata catataccta tttctgcttc taatgcttta ggatttatat tattaaaccc
    61261 catattccca ataactttat tgtctctttt atatactata gcaaagtggc caattttccc
    61321 ttttttatat ggatttagaa aaaatgattt tataaacttt cttgtatctt ctaaagattt
    61381 atgctcacta aatggtaaat gttttactac tattttattt ttataacagt caaaaaaatc
    61441 ttcaacatca tctaatgatg caggtcttag tatacaattt ctagtttcta aggttttcat
    61501 ttgaggatat tcagaatcag acatttctaa tcccccttta tacattaatc taaatatact
    61561 gttcatattt aaaatacgat attaattata taaatgttct caaattaacc ttatttttat
    61621 ttttaataaa aactaattac tatatgaatc tttcaaattt acgaatttat ctctattttt
    61681 atattaaatt acttgtgaaa tatgtattct aaattatata ttattagata tcatgcttaa
    61741 aaattctttg gttgctaagg ataaagatat atcttttaaa tggcagtaac ctatatttct
    61801 cttaggtatt ggctctttta tatctaattt aaacaatttc tcattttcca aataatctat
    61861 gctaaattct tctataacac aagacactcc aagatttata tacgcaaatt ctaaaagtaa
    61921 ttcatgagaa cccaattcta tatccgggtt tagttttata ccttttgata aaaaatattc
    61981 atttacataa agtcttgaat ttgctttatt ttctagcatt attaaaggca atgtgtttaa
    62041 ttcttccaat gatattttcc tacctttgta ttctatgtaa tcatttgcac atacaaatat
    62101 atcatgtatt tcaatacact caacaatatt tagtgtatca tcttctattg gcatgtttat
    62161 aattgctata tctatattcc catctttgag taaagatata agttctctac tcgttctatt
    62221 tattgtagat acatgtatat gtggatactt cttatgaaaa atttctaaat attttaacaa
    62281 gaaaaaacgt gcagctgtat ctcctactcc tattttcaga gaaccgtatt ctaatttttt
    62341 aaatttattt attctatctt ctcctgatga tattaacttt aatgcagtag tgacatgttc
    62401 acttagaatt ttcccctctg gagttaattt tactccctta ttgtttctat aaaataataa
    62461 agtatctaat tgttcttcta attgctttat tgactggctt acagctggct gtgacataaa
    62521 caattcctta gcagcaagag aaaaactctt attatttaca actgcattaa aaaccttata
    62581 aagttcaagt tttatattca tatataatta ctccttatgt caaatattag tattatttat
    62641 tttacttata attatatttg aattataata tacatggtac taatatgaca atattaattc
    62701 tttaggagga ttttagataa tggatagatt agttggaact gtttcaagag gggttagagc
    62761 acctattata agacaaggag atgacttagt taaaatagtt gttgattctg ttttaaatgc
    62821 gtcaaaaagt gaaaactttg aagttaggga caaagatgta attgccgtta cagaagctgt
    62881 tgtagctaga gcacagggaa attatgctca cgttgacaat attgcaaaag atgtaaaaga
    62941 taaatttgga gatgatactg ttggggttat attcccaatt ttaagtagaa atcgttttgc
    63001 tatatgttta aaaggaattg caaaaggatg tagaaaagtc gtattaatgt taagctaccc
    63061 ttctgatgaa gttggaaatc acttaataag tattgatgaa cttgatgata agggaataaa
    63121 tccttggtca gatgttttaa cagaagaaaa atacagagaa ctatttggat ataacaagca
    63181 tacatttact ggtgtagatt atgttgatta ttataagaat ttaatcaagg attgtggtgc
    63241 agaggtagaa atagtatttg caaacaatcc aaaaactatt cttaactata caacaagtat
    63301 tttaacttgt gatatacata caagacaaag aacaaagaga attttaagac aaaatggagc
    63361 taaaaaagta tattcacttg atgatataat gacagcaagt gtagatggta gtggttacaa
    63421 tgaccagtat ggacttttag gttcaaacaa agctacagaa gaaacagtaa aattattccc
    63481 tataaattgt gatgaagttg taaacaaaat ccaaggtaat ataaaagaaa taacagggaa
    63541 agatgtagaa gtaatggttt atggagatgg agcttttaaa gaccctgtag gtaaaatatg
    63601 ggaattagct gacccagtag tatctcctgc ttatacaaaa gggctagagg gaactccaaa
    63661 cgaagtaaaa ttaaaatact tagcagataa tgattttgct gacttatctg gtgatgagtt
    63721 aaaagaagca atttcaaaat acatagttga aaaagataat aaatctgatg atttaacagg
    63781 aaacatggtt tctcaaggta ctactccaag aagattaaca gaccttatag gttctttagc
    63841 agatttaaca tctggaagtg gagataaggg tactcctatt atttacatac aaggatattt
    63901 cgataactat acaaaatagt atgttttaat aagatttaat ttttttggat tatatatatt
    63961 aacatgaaaa tttattaaaa cctcttaata gatatgactt ttcaagagtt atatctatta
    64021 agaggtttat ttaatgttca cagataagat atatttaaat aatttaaaca agattgtcag
    64081 aaagttatat aatcttaaaa atttatataa ctctaattct tatttatttc tgaaataata
    64141 tcactcttca aaatttgtct tactggaata attacagcaa ctatactaat agtgatattt
    64201 aaaataatca tacctatgta tagaggtgta tttattttaa gaattggatt tatatataaa
    64261 tatacacgtt ttaccataaa ataaatcata aattgtccaa taacacttcc aattaccatt
    64321 ataatacttg catagaatcc atataaaaaa ccttctctta tcatcataag taaaaactta
    64381 ttatccgtaa tacccatagc tcttaaaatt ccaaactcat gtcttcttga aaaaattaag
    64441 tagcttactg tattgattat atggaacaaa cttattgcaa gaagtacaaa tacaatacca
    64501 tatataaata agagtttttg atttaagtag gtattatttt tttcaatagc aatggtataa
    64561 tctgttacga tacatctttt tacatcactt acaacacttt ttatgttctc agacaattcc
    64621 tcactgttac tatcttctgt cttttttata cttactgaat tataattttc gatatcaaag
    64681 ttatttttca tttgcccatt cgtcataact atatccatac cataatcacc aatgaaatat
    64741 atgttatttg ccatcacacg ttttacagta gcagcaacta caaattcttt tgttgtataa
    64801 tcattcttat tttcttcttt aaatttaaca atttcatctg ttggtttcat tgtctttggc
    64861 actttaactt ttattgtatc tcctggattt atatcaactg catcatacat cccagttcca
    64921 tccataggta gtctaactat aaccttatta tttttaacca tatcatgggt attaatctcc
    64981 ccatctataa tataagaatt taatccttct aacataccat tgttataacc ataaatattt
    65041 gtttttaaaa gatagccacc ttcattttgc tttgtacaaa taccatcaaa aaaagtcttt
    65101 atcctgtaat ctttttcaag aggtttaaag aaatttttcc aaaatagttg ttcttcttta
    65161 atttgcatag ctccaaataa atatcttact ggatagactt ttgatattcc ctcaacattg
    65221 tttaatcttg aaattttatc ttttggtata cctacatcaa aatctgttgt ttgcatagtt
    65281 atttggtagt cagaatttaa accatcatca gctttcattg taagttcatt attattttta
    65341 gtaagctgta tagagtaact agaacataga aatataattc caccaagtga taatgaaaac
    65401 aaaattccaa taaacattcc ctttttttga gtcatgtatt tatgagataa tacttttgtc
    65461 atgttagagt gttttacact ttctaatatt ctctttttct tattagtaat cttaaagttt
    65521 ttcatagatt ctatactaga gtatttattc aactgatgta caacacgtac agcaattaaa
    65581 attaacaaca ataagataaa taaaaagcct aaataaatag atttttcact tataaaaaat
    65641 gctcctggaa caatatcact actcaaaaat atatgtggaa attgttcata tattgtagac
    65701 gctactccaa tacctaagaa gcaacctata ggaaatccaa caataaaaag taaaaataac
    65761 tctaaaaata gtagaaataa aagttgttta ttattagcgc ctaatacttc taaaacccca
    65821 tattgtgata ttctctgaag tattgaaaca tggaaaatac tatatactgc tgctgcacca
    65881 aacactccaa caagcaatat aattgcattt gtatttagtt ctaaattttt taagaactca
    65941 aatatattat atttttctat attttttggt ggttttactc caaattcaac tgaaagagac
    66001 cagtttatat aagttttttc ttttgatatg ttgaattttt tcataaattt ataattttgc
    66061 tttttaatat ttttattttc atcatatttt acataaacta tataagaatc cttttcttta
    66121 ggctgaaaat aatcgtttac aaatccctcc agagcagttt ctgggtttga aagacttgat
    66181 aaaataccac ataacttata gattttacca tctaaattga tttctgttcc aatttctttg
    66241 tttacattta aattgtttag agaatatgtg tcaagtacta tctctccatc tttactagga
    66301 agagttcctt ttaataattt tctacctgtc atgtctaaat atgaagaatc agcatttaaa
    66361 aattctataa taaaaggttc atctatctga tttttaggtt ttgtaacgcc aagtctatct
    66421 acagtgtatt cctttgattt tttattttct ttaattttat taagttgttc cttatctact
    66481 ttatagaaat aatgataatc tccactatct tcccttatct tttcaattct acttttgtct
    66541 gcactatgaa ctaaagaacc aattcccgtc aataatgcta ctgatagtac tatgcttaaa
    66601 actatagata tagattgttt tttataatgc tttatatatt gaattgaaaa ttttactaga
    66661 gacatcatag taattcacca ccagtattaa ccacaactcc atcttcaata tggataattg
    66721 agtcgcatac ttgtgctata ttttcattgt gtgttaccat aagtatcgtt tgatggaatt
    66781 ttgcactact ttcttttaat aaacatacca cttccattgt agttttacta tcgagatttc
    66841 ctgttggttc atctgctaat ataagcgcag gtttatttgc caaagctcta gcaattgcta
    66901 ccctctgttg ttgaccacca gatagttcgc ttggaaattt taaacgctta tcccaaattc
    66961 ccaatgtctt catcagcatt tcaatatatt catggtctat atggtttcct ttatctaatg
    67021 taacaggaag agctatatta tcataagtgt ttaatacggg cattaaactg tagttttgaa
    67081 atataaatcc tatatttctt cttctaaaca ctgtcaaatc tttttttcct attttagata
    67141 tgttctttcc tttaattatt acatcacctg aggttggatt atctaatcca cctataatat
    67201 ttaacaatgt gctttttcca gaaccagaag ttccaactat agcagtaaat gtatctcgtt
    67261 caatttctat acttactcca tttaaggctt ttactttagt ctcacctttt ccatagtgtt
    67321 ttactaaatt attcgtcact aatatactca tttaatttct cctttcaata cagatatatg
    67381 ttctataaaa tattgtaagt gacgataatt acactcatcc tacaaaatta gtttaaactc
    67441 ttacaattct gtaagaagat actaaaacaa catccacttc ctagtttaga ttctactata
    67501 atgtttccat tttctttttc taaaattaat cttgtaagat atagaccaat ccctgtgcca
    67561 ttttggtttt caacattttt acttctgtaa aatcttttaa aaatatcatt tagttctcta
    67621 ctatcaatac ctattccctc atctttaata gtaatcttcg tatatagttc catttttatt
    67681 acagaaatag taatcgtgct atctgatggt gagtatttta ttgcattttc taaaacattt
    67741 acaattgctt ctatggtcca ctttttatta tgaactaatt ttatgtctga aaatggttcg
    67801 agatttatcc ttatattttt agcttctgct ttattaaaga tagtactgat actttgaata
    67861 agagtatctt taattagtag ttcttctact tcaaattcta taactccatc ctctaattta
    67921 gtcattttaa ataaggattg taacagccag tcaatcttaa aagtttcatt tttcatcttt
    67981 tgtaaaaact ccttcttctg taatgtggaa atatttatat tctccagaag ttcacaataa
    68041 atggttatat ttgaaagtgg agtttttaat tgatgtgaca tatttgatat taaaccttta
    68101 atcgcttctt tttctgactt tgactgttct acctctattt ctatcatatc cttaattttt
    68161 tttacttggt gagaaatttt tgacgctctt gtatcctttg tatctggata agtaacttct
    68221 ttaccttcca atatttgatt taacatcaaa tttaattcat catatctttt tcgatttatt
    68281 ttagtagata ctataaatat aacaactgta catattataa aggctaatat aaacatatta
    68341 ttctacctca ttccataaat aacccattcc ataagctgtt tttatatatt ttggattaga
    68401 tgggtcatcc tctactttta ctctaagtct tcttatattt acagatacaa tattatcatc
    68461 aacataattt tcttcgctgt cccaaatatg gtgtaaaatt tgttccttta ataatatttg
    68521 acctttattt tcaattagat aacttagaag cttatattct acagagctaa gttccaaaca
    68581 ttctttattt ttatatactt ttagtaactt ttggtccaat tttattccat tggaatatat
    68641 tactttatta ttcgattttt ttcttattgc tactttgact cttgccttta atacagataa
    68701 agaaaatggt tttgtaatat aatcatcaag ccctatttct aatcctttta cttcatcaat
    68761 ttctgagtct cttgcagtaa gcattataat tggtatatct gacttttccc ttattatttg
    68821 acatacatca aatccatctc catctggaag attgcagtct aagattataa aatctatatt
    68881 tttactatta aatatattta atccttcttg ttttgtataa gccttaaata cattaaatcc
    68941 atcattctca aatgcataaa aaagaccttc atttaagttt atatcatctt caataattaa
    69001 taagttcaat tttactctcc tttagatttt ttagataaat aattattttc ttgcattatc
    69061 atactaaaaa tattttaatt ttatttattt cactaaaaag attgtattta aatttgtttt
    69121 caaaataaaa a
//
LOCUS       DAMJWS010000010        68116 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMJWS010000010 DAMJWS010000000
VERSION     DAMJWS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.010"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(94..2064)
                     /gene="ftsH"
                     /locus_tag="PC399_000694"
     CDS             complement(94..2064)
                     /gene="ftsH"
                     /locus_tag="PC399_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2591571.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2098..3486)
                     /gene="tilS"
                     /locus_tag="PC399_000695"
     CDS             complement(2098..3486)
                     /gene="tilS"
                     /locus_tag="PC399_000695"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF2591572.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3669..4097)
                     /locus_tag="PC399_000696"
     CDS             complement(3669..4097)
                     /locus_tag="PC399_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF2591573.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4114..4920)
                     /locus_tag="PC399_000697"
     CDS             complement(4114..4920)
                     /locus_tag="PC399_000697"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF2591574.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5122..5607)
                     /locus_tag="PC399_000698"
     CDS             complement(5122..5607)
                     /locus_tag="PC399_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2591575.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5658..6317)
                     /gene="spoIIR"
                     /locus_tag="PC399_000699"
     CDS             complement(5658..6317)
                     /gene="spoIIR"
                     /locus_tag="PC399_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2591576.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6462..7142)
                     /locus_tag="PC399_000700"
     CDS             complement(6462..7142)
                     /locus_tag="PC399_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2591577.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7126..8016)
                     /locus_tag="PC399_000701"
     CDS             complement(7126..8016)
                     /locus_tag="PC399_000701"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF2591578.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8134..9684)
                     /locus_tag="PC399_000702"
     CDS             complement(8134..9684)
                     /locus_tag="PC399_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2591579.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9778..10044)
                     /locus_tag="PC399_000703"
     CDS             complement(9778..10044)
                     /locus_tag="PC399_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF2591580.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10192..11052)
                     /gene="yabG"
                     /locus_tag="PC399_000704"
     CDS             complement(10192..11052)
                     /gene="yabG"
                     /locus_tag="PC399_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF2591581.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11136..12074)
                     /locus_tag="PC399_000705"
     CDS             complement(11136..12074)
                     /locus_tag="PC399_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF2591582.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12184..12345)
                     /locus_tag="PC399_000706"
     CDS             complement(12184..12345)
                     /locus_tag="PC399_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591583.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12355..12852)
                     /locus_tag="PC399_000707"
     CDS             complement(12355..12852)
                     /locus_tag="PC399_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2591584.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(12967..13677)
                     /locus_tag="PC399_000708"
     CDS             complement(12967..13677)
                     /locus_tag="PC399_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF2591585.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13853..14383)
                     /locus_tag="PC399_000709"
     CDS             complement(13853..14383)
                     /locus_tag="PC399_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591586.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14400..14630)
                     /locus_tag="PC399_000710"
     CDS             complement(14400..14630)
                     /locus_tag="PC399_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591587.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14645..16039)
                     /locus_tag="PC399_000711"
     CDS             complement(14645..16039)
                     /locus_tag="PC399_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2591588.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16165..17418)
                     /locus_tag="PC399_000712"
     CDS             complement(16165..17418)
                     /locus_tag="PC399_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2591589.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17469..18623)
                     /locus_tag="PC399_000713"
     CDS             complement(17469..18623)
                     /locus_tag="PC399_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2591590.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(19183..19752)
                     /locus_tag="PC399_000714"
     CDS             complement(19183..19752)
                     /locus_tag="PC399_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2591591.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            19955..20182
                     /locus_tag="PC399_000715"
     CDS             19955..20182
                     /locus_tag="PC399_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2591592.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20401..21144)
                     /locus_tag="PC399_000716"
     CDS             complement(20401..21144)
                     /locus_tag="PC399_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2591593.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21312..22184
                     /locus_tag="PC399_000717"
     CDS             21312..22184
                     /locus_tag="PC399_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2591594.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22184..22849
                     /locus_tag="PC399_000718"
     CDS             22184..22849
                     /locus_tag="PC399_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2591595.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22870..23727
                     /locus_tag="PC399_000719"
     CDS             22870..23727
                     /locus_tag="PC399_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2591596.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23883..26429)
                     /locus_tag="PC399_000720"
     CDS             complement(23883..26429)
                     /locus_tag="PC399_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2591597.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26429..27115)
                     /locus_tag="PC399_000721"
     CDS             complement(26429..27115)
                     /locus_tag="PC399_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591598.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27198..28187)
                     /locus_tag="PC399_000722"
     CDS             complement(27198..28187)
                     /locus_tag="PC399_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2591599.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28174..28860)
                     /locus_tag="PC399_000723"
     CDS             complement(28174..28860)
                     /locus_tag="PC399_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591600.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29360..32566)
                     /gene="carB"
                     /locus_tag="PC399_000724"
     CDS             complement(29360..32566)
                     /gene="carB"
                     /locus_tag="PC399_000724"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2591601.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(32759..33808)
                     /gene="carA"
                     /locus_tag="PC399_000725"
     CDS             complement(32759..33808)
                     /gene="carA"
                     /locus_tag="PC399_000725"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2591602.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(34065..37271)
                     /gene="carB"
                     /locus_tag="PC399_000726"
     CDS             complement(34065..37271)
                     /gene="carB"
                     /locus_tag="PC399_000726"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2591603.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(37324..38367)
                     /gene="carA"
                     /locus_tag="PC399_000727"
     CDS             complement(37324..38367)
                     /gene="carA"
                     /locus_tag="PC399_000727"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2591604.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(38393..39109)
                     /gene="pyrF"
                     /locus_tag="PC399_000728"
     CDS             complement(38393..39109)
                     /gene="pyrF"
                     /locus_tag="PC399_000728"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF2591605.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(39485..40120)
                     /locus_tag="PC399_000729"
     CDS             complement(39485..40120)
                     /locus_tag="PC399_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF2591606.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(40144..41058)
                     /locus_tag="PC399_000730"
     CDS             complement(40144..41058)
                     /locus_tag="PC399_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591607.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            41659..42888
                     /locus_tag="PC399_000731"
     CDS             41659..42888
                     /locus_tag="PC399_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2591608.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWNIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(43093..45474)
                     /locus_tag="PC399_000732"
     CDS             complement(43093..45474)
                     /locus_tag="PC399_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF2591609.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(45446..46171)
                     /locus_tag="PC399_000733"
     CDS             complement(45446..46171)
                     /locus_tag="PC399_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF2591610.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKYVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(46161..46616)
                     /locus_tag="PC399_000734"
     CDS             complement(46161..46616)
                     /locus_tag="PC399_000734"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF2591611.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(46898..48079)
                     /locus_tag="PC399_000735"
     CDS             complement(46898..48079)
                     /locus_tag="PC399_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2591612.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(48069..48761)
                     /locus_tag="PC399_000736"
     CDS             complement(48069..48761)
                     /locus_tag="PC399_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591613.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(48895..49611)
                     /locus_tag="PC399_000737"
     CDS             complement(48895..49611)
                     /locus_tag="PC399_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2591614.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            49736..50470
                     /locus_tag="PC399_000738"
     CDS             49736..50470
                     /locus_tag="PC399_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2591615.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            complement(50755..51606)
                     /locus_tag="PC399_000739"
     CDS             complement(50755..51606)
                     /locus_tag="PC399_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2591616.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGTL
                     IKSDTIESKSERIKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(51632..52570)
                     /locus_tag="PC399_000740"
     CDS             complement(51632..52570)
                     /locus_tag="PC399_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2591617.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(52673..53323)
                     /locus_tag="PC399_000741"
     CDS             complement(52673..53323)
                     /locus_tag="PC399_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2591618.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKTKEAVEKYREYFADKGIFENKIYENIKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            complement(53473..53661)
                     /locus_tag="PC399_000742"
     CDS             complement(53473..53661)
                     /locus_tag="PC399_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2591619.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(53768..54952)
                     /locus_tag="PC399_000743"
     CDS             complement(53768..54952)
                     /locus_tag="PC399_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2591620.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGLNQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLSVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(55094..56011)
                     /locus_tag="PC399_000744"
     CDS             complement(55094..56011)
                     /locus_tag="PC399_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF2591621.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTETGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(56033..56761)
                     /locus_tag="PC399_000745"
     CDS             complement(56033..56761)
                     /locus_tag="PC399_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2591622.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(56957..57157)
                     /locus_tag="PC399_000746"
     CDS             complement(56957..57157)
                     /locus_tag="PC399_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591623.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(57282..57662)
                     /locus_tag="PC399_000747"
     CDS             complement(57282..57662)
                     /locus_tag="PC399_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2591624.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMRQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(57935..59293)
                     /locus_tag="PC399_000748"
     CDS             complement(57935..59293)
                     /locus_tag="PC399_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2591625.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDGEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGLLRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            59741..60193
                     /locus_tag="PC399_000749"
     CDS             59741..60193
                     /locus_tag="PC399_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591626.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRIFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(60350..60967)
                     /locus_tag="PC399_000750"
     CDS             complement(60350..60967)
                     /locus_tag="PC399_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF2591627.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(61390..62202)
                     /locus_tag="PC399_000751"
     CDS             complement(61390..62202)
                     /locus_tag="PC399_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2591628.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            complement(62278..63696)
                     /locus_tag="PC399_000752"
     CDS             complement(62278..63696)
                     /locus_tag="PC399_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2591629.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            complement(64180..65097)
                     /locus_tag="PC399_000753"
     CDS             complement(64180..65097)
                     /locus_tag="PC399_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF2591630.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            65359..65736
                     /locus_tag="PC399_000754"
     CDS             65359..65736
                     /locus_tag="PC399_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2591631.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(65892..66341)
                     /locus_tag="PC399_000755"
     CDS             complement(65892..66341)
                     /locus_tag="PC399_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2591632.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(66508..66912)
                     /locus_tag="PC399_000756"
     CDS             complement(66508..66912)
                     /locus_tag="PC399_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF2591633.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(67025..67891)
                     /locus_tag="PC399_000757"
     CDS             complement(67025..67891)
                     /locus_tag="PC399_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2591634.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRIKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
BASE COUNT        21710 a        12295 c         7419 g        26692 t
ORIGIN      
        1 aatataattt gcttattaat ttaatattat actttcagtt ttagtattta atccacatca
       61 gataataatc ttaaaatgac caaggtctta aaactattta tctttatcag atttatcttc
      121 taaattttta tttatatcaa taacattgtt tctttcttct tcatcttttt ctaattttat
      181 agttaattgt tcttcaactt ctttagaaga attttcctct gtaactgcat tttcaatttc
      241 attcagaggt aactctttat taaatgcttt tacaaattgt tctgcatcta aagtttcata
      301 tataagtaaa gcttgtgcta catactctaa tctatccata ttttcttgta atatacttct
      361 tgtcttatca tatgcaccat caactatacg ctttgtttct tggtctatct caaaagcaac
      421 ttcttcagaa taatttctct tacttgaaaa actattacct aggaatactt catcatcact
      481 gtcaaatgac attggaccaa gtttagaact cattccatac tttgttacca tacttcttgc
      541 agtagctgta actctttcta agtcatttga agcacctgta gatacatcct taagtactag
      601 ctcttctgct actctaccac caagtaaaac tacaatattt tctttcattt cattttttgt
      661 agcatagaac ttatcttcaa caggaagttg cattgtaaac cctccagctc ttcctcttgg
      721 aactattgta acttgatgaa caggacttac ttcttcaagt acatgggcac aaactgcatg
      781 ccctccttca tggtatgctg taagtcttct ttctttctca cttattactt tcgatttttt
      841 agccacacct gctataacct ttgttatagc ttcttctata gtttccatct taattttctt
      901 ttctcttttt ctagctgtta atattgcagc ctcattcatt aagttctcta tatcagcagg
      961 agtaaatcct ggtgttcttc tagccaagac atccattttt acatcatcac ttaatggttt
     1021 atttcttgag tgaactttaa atatagcctc tctacctttt acatctggtg taccaacaac
     1081 aacttgtctg tcaaatcttc ctggtctaag taaagctggg tcaagtatat ctggtctatt
     1141 tgtagctgcc atgattatta taccttgatt taccccaaat ccatccattt caactagaag
     1201 ttgattaaga gtttgctctc tttcatcatg acctccacca agacctgcac ctctttttct
     1261 accaactgca tcaatttcat ctatgaatat gatagctgga gcacttttct tagcctgttc
     1321 aaataaatct cttactcttg aagcaccaac cccaacaaac atctcaacga aatcagaacc
     1381 acttatactg aaaaagggaa ctcctgcttc tcctgcaact gctcttgata gatatgtttt
     1441 accagttcca ggaggtccta ccataagcat tccttttggt attcttgcac ccaattcaat
     1501 atatttctta ggattcttta agaaatctac tacctcctgc aaatcttctt tttcctcatc
     1561 tagtccagct acatctttaa atgtaactct agttttttca tcatctttat gaaccttagc
     1621 ttttgatttg ccaaaactca taacctttcc gcctccacct tgagactgat tcataaacac
     1681 gaaccaaata atcaccatga agaatattaa cagtaatgta ggcaacattt cgacaaacca
     1741 aggtgtagat ggcttagctt caccaccaaa agttagtttc ccagccttag cttggtctaa
     1801 cacctcatct gcaagtttat ttcccatcac ttctgttgga acatatgact taaactttgt
     1861 atttgtatct tttatagtac cttctacaga agtttgattt acaaagtata atcttgatat
     1921 attctcatct gttaattctc tatatacttc agagaatttt aaatctttta ttttttctgt
     1981 aggcttacct gagaattgaa ctatacctac tataattata aaaacaagta gataaaaacc
     2041 tgcccctttt agcaacttat tcaatataga agccctcctt tcatacattg ctttgcccta
     2101 taatttgtta aaattaattt ttaatacttc ttttgtgttt tcatcaattt tataattttc
     2161 actgatttta taatctccaa cacatattat tccttcacta tctgtgatta taggaatttt
     2221 acatctttct tctcttggga tttttaaatc tatgaataaa tctttcactt ttttacttcc
     2281 catagcaagc tttatcttat caccttgcct tctgcttctt attactatac ccccttttac
     2341 cttattaaaa tcaaaccctt tagatgaatt gtctaatttc atactcttat acctatctat
     2401 gctcattact tgggtctcaa taatttgttt taattctttt attttaataa acccattact
     2461 aggtacattg taataaaatt caatttcctc attaacaatt tcctcattcg ttaatattat
     2521 actgtctttt tttctataaa caaatatatt tcttggaaga gttaacattt tatttacttt
     2581 tgaattatct tctaaagaca ttatatcttc tatatgtctt tggtcaacaa aattagtatc
     2641 tcctagtata tgttttatgg aatttctaag aactctaact ttgatagcat catgtaagca
     2701 aacaaaatcg tctaaattta tctctacaaa gcctttttct tttatatttg aaacttctct
     2761 aaatttagct tctgcctctt tctcaatata atcgttatca ctttttaaac tattgctcat
     2821 cctcactata gattctatta catttgaatt aaaattatcc ttcatatatg gtaaaaggtc
     2881 taaccttatc ttatttctag tgtatatatt ttctaaattt gttttatcta tcctcggatt
     2941 taaattataa gcttcacaat actcctctat ctcatttctt tccacgtcta atatgggtct
     3001 aattatgcaa ttatctctaa tataatcaat tccctttaag ccttttaaac ctgttcctct
     3061 catgatacgc atcataaccg tttcggcttg gtcattgaga ttatgaccta ttgctatttt
     3121 attggcttta atcttatcct taatttcata aaacatttca tatcttagct tccttgctcc
     3181 ttcttctaat gataatccct cattttcaca atattttggt acattaatag actttacaaa
     3241 gaatataatt cccatatcct cacaaagttt agatacatat aatgcatctt tttgagcttc
     3301 tatccctctt atttgatggt ttaaatgtgc tgcatatatt tctatattaa aatctttttt
     3361 taatctattt agtacatgca ataaacaaac tgaatcaggt cctccagaaa gacctaaaac
     3421 tattttatcg cctttttgta ttaaattatg tttatttata gtacttagta ccttatcaaa
     3481 tatcatattt atccctctta cacttagttt tattcacttt taagtatact ttctataaaa
     3541 aaacgctaaa cccttgtatc aattttaaga aattaaagtt taatataaat ataataatac
     3601 caaataaatt aaataaaagg tagttttttc actaccttta taaaatttct ggaaaatagc
     3661 catagttatt atttagtacc tatgtatata tctattttat tcctacctaa aaataaatct
     3721 tgtaccttta tattatagtc tattttcaat tttatgtgtt ccccttcttg tatatttata
     3781 tctaattctc ttgtatcaat atcaattaat aaagtacctt gtggggtcac atactttgta
     3841 gtactacttt ctccacattt aaacagcatt gttgagttaa tagcaccaaa ctttttgata
     3901 ctaacttgat ttttaagtat ctttatagta ttagttattt ctatatcttc ttctttttcc
     3961 ttgtaaataa catatattcc atcattttta taaaaaattt taccaaaggc agtcatctct
     4021 atggtgtcca tatttccttt ttcatcatat tgtctagtat ttatacttat ttttacactc
     4081 aacgattttt cctccataga ttcttcatat aaactagtat ttttgtattt ctactttttg
     4141 aatcttatca atttttttct ttaagaactc tcttgctata tctgaaaatt tttcaggtat
     4201 atctgataca taatattgat aagttccatg aatttctgta ttgtttataa tattttgcac
     4261 ctctaaaatc ttttttaaat cctttgcagt ctctttagct ggattaacaa gttttatatg
     4321 ctctccaact tcttcaccta ttgttctctt taaaatagga taatgagtac atcctaacac
     4381 taaagaatct atattttttt ctttcaattc ttgtaaatat atctttgctg tcagtttagc
     4441 cacttctgta tttgcccatc cttcttcaac aataggcaca aataaaggac atgctttatt
     4501 aacaatttct atactctcat ctattttaga tatttccaag ttgtatgctt ttgaacttat
     4561 agttccttca gttcctatta ttcctacaat tttgtttttt gtagaactta cagctgttct
     4621 agctcctgct tctataactc caattatcgg tatatcatat ttttcttgag cttcttttaa
     4681 gcttcttgca gtagcagtgt tacatgctat aactatagct tttacacctt ttgtttttaa
     4741 aaagtttata gcttgaaaag tatatttaat tatagtttct ttggatcttg aaccatatgg
     4801 tattctggca gtatctccaa agtatattat atcctcattt ggtaatatct tcattatctc
     4861 ttttagaact gttaatccac ctaatcccga atcaaaaact cctattggtt tattgttcat
     4921 ctttatacaa cacctacttt acatttttgt gtttaacaac aacttattta tcgctaaaat
     4981 ttatatttat attcataatt aatatattat tacctaaatg attattatag tttattttac
     5041 acaaaaaata aaggagctat cttaaattag aaaataattt ctacaaaaag acaactcctt
     5101 tcactactta aaaatttaaa tttattttgc aaacacatga tttcctattc tcatataagg
     5161 attcaatcta tttatccatt tgcaagtggc tatttctgga ttccaaaaat atactgcttt
     5221 attagttggg tcagttccat aaagagcttc ctgagctgct ttacgtgctg agtctgttgg
     5281 tgccatcttt attgaaccat caagcacaac tgagaaagca tttttttgat atatgacttc
     5341 ttctattgtg tctggaaatc tagggtccaa tactctattt attactacag ctgctacagc
     5401 aacctgtccc tcatagcttt ctcctctagc ttctcctgca accaacttag ataataacat
     5461 caagtcatta tcagataaat ttattacttc tttctttgct atattttttt cttgtttgtt
     5521 tactttctta atattttctg ttggttcata tgcaaatacc tctttaatag gcataaatat
     5581 aaaagtcaaa gctaatagtg tagccatcag tttactccac ctgtccatga ttaaaaccac
     5641 tccttacatt cttattttta aaattttttc tctaaatcct tgactacttc aagtacttta
     5701 aatttaattt ttactctact tgtttttttt ggtgtatctt gttttattaa cttatattct
     5761 ttatcagtta aaacttcctt caacttatca tctgtagatt tatctatcac tccattagat
     5821 tcatcaacaa aacatagtgg agggaacata acacaccacc agttttttcc ttcacctttc
     5881 ccaatgataa tctttaatgc tttatattca ccagctggaa gaactatatt agaatattgt
     5941 ttagtaggga aattgctata ttgtatacct acctttacac tgtaattata tccattttta
     6001 agaataatat ttttacttat ttcttccaaa ctactatatt ccttttttat aatagcttca
     6061 ctttctttta tactttttga attttgaagc tttggctgta aatattttat tacttcatct
     6121 ctaactttta gtttaagttc ttggtcttca tctgtattgc tgtttgctat aacatgaaat
     6181 cttattaatt tatctttata atcttttgat atattgtcta ctttttttac ctcaccattt
     6241 attactattg tcattattga tatgactgat ataagactta atattaatag tcccaatcta
     6301 atcttaattt ttctcattga aaattccccc tcaagataaa tttatatcat caattatgtc
     6361 caattaaatt taatttattc ataatatttt ttaatactaa taaaaaaagc tatcttaaaa
     6421 tgattacaat cattttaaaa tagcttttta aaccttatat tttataagtc tttactaatc
     6481 atagccttat ctatcataaa ctgctctgct ttttctatat gctctgtagc atacttcttg
     6541 gatagctcac tatcaccttt aattatagca tcaagtaaat atttatgctc ttcaacaata
     6601 ttatgtgatg tatcataagc agacatatag gtaactctaa atctatatac ttgttcccac
     6661 aatgagttta ttaattggat taatttttta ttattagttg ctttaaatat acattcatga
     6721 aattctacat caagttttaa taacgcatct acatcggatt ccaaagtgct ttttttaaat
     6781 tcttctgata tatctttaag ctttttaata tcttcatcac taattctttc agcggcaaga
     6841 tatgctgcta agccttctag gcttgctctt acttctaata catccataat atcctttaat
     6901 gacatatttg ccacatacgc accttttcta ggcaacataa cgactaaacc ttctaattca
     6961 agctttctta ttgcctctct cactggagtc ctacttactc ctaattgttc tgctagttgc
     7021 acttccatta gcctttgacc aggctttaat tttccttcta agattgcttc tcttaaattc
     7081 tcaaagacta catctcttaa tggcttgtaa ttatccaagt tcagtttagt taaattctcc
     7141 acaaatttca accccttttt gactgctatt aacaacatac acctgtttat atttttttaa
     7201 taattctttt tttccaatta gagcatcttc tttattttta aatagtccaa aaactgtagg
     7261 accacttcca ctcatcatag aacctaaagc attatttttc atcattactt gttttatatc
     7321 acttatttct ttgtgtttac ctatagtaac attttcaaga atatttacca tgctttctga
     7381 tacagctttt atatcttcag attttaaaca ctctatcaaa tatttattat ttggtctttt
     7441 tttaatatta ttcaaatcta atccttgata aacttcttta gtagaaacga ataagtctgg
     7501 tttgcatatc aatatatttg tatcacatgg taaacctttg atgtttgtta gcttttctcc
     7561 aataccttga gctaaggcag gtcttcctga tatacaaaaa ggaacatctg cgcccaaatt
     7621 aagtcctatt tcttttaatt catcttcaga taatctaagt tcccataggt gatttaaacc
     7681 tactaatact gcggctgcat tagaacttcc tccagccatc cctgctgcta caggaatatt
     7741 tttttctatg aatatttcta cacctttttt tatataaaat ttatttttaa gtattttggc
     7801 agctttataa acaatattat cttcatctaa aggaatatct aaactagtgc tttttacctt
     7861 tatttcatct tcgtctaatt cttttatctt tactatatcg tataaatcta tcgtttgcat
     7921 tatcatttca acaaaatgat aaccgtcttg cctttttccc aagacatcaa tggataaatt
     7981 tatttttgct ctactcttta gtcttataaa atccattcta tcctccttgt ttaaagaata
     8041 atgcatcact aacaataata ttatactaca aaaaataaaa taatagggta ttataatacc
     8101 ctattatact taattacttt aaatttttta atattaatct actaatacga cttttttttc
     8161 taaaataaga cacttaccag gcttaattgg ttcatcaagt tttatatcat taagttctgc
     8221 aatttcattt tctgttgtat tgtatttttt tgctatattc cagaaagtat ctccttcttt
     8281 gcatatatat acgattatgc taggagcttt agataaatca tatacacctt ggtcttctcc
     8341 cttgataata aaattttctg ctttcttatc taatgcttca gtaaatctct taattttgat
     8401 aatcaagtct atctggtccc tattcaaatc aacttcgact ttatcaatac atgctgtatt
     8461 aaacacagat gctgtatcag ttagattatc catttctata tcatgttcaa atggaatttc
     8521 ctcactaatt ttataaacca attttaaacc ttcaactggg acaaataaaa tttcaacttt
     8581 tatgattcct tgtataatgc ttttatttcc ttcaatataa ctattttcta tagacatggt
     8641 tggacaaacg cttacaatat cttttatttg tatatcatca ttatcatttc ttatgccttc
     8701 tctaaccata aatgtttctt cactacgtct taaagtctta tttaattgaa taggtttgtg
     8761 gtcaaatttt attatctttt gaggcgaata tgcatcttgt aatacttctc ttgtaacttc
     8821 atctgtaact tttaccttac aacaagccat acaatctatt tctaaaagac ctgtgttgct
     8881 ttcactattt tgcttgaaaa tatgattaaa atctgacata gacaacaata cttcttcagt
     8941 cataccatca ctaactccag ggacttctac aaattgagta aactcaatac ctactctatc
     9001 caactctaca agttcaccct catatgtaca agctaacgga tttatttcta atactccacc
     9061 aataataact ttattgtcag tcactctact ttcttttact ttaacacatg gatttaaact
     9121 tattatagat tgaatttcct ctgtgtttat tgttatagta tctcttatag aactttctgc
     9181 tttttctatt ccaactatat cttgaaaaca aatttccttt ctgtgctttt gtatgccttc
     9241 cacttgcgcc acatctttta caatgtctaa cctttgcttt tcaaacaaac ttccccttat
     9301 attcataaga gctcctactt taatctttct ctcattcatt atagtacaat ccatatgctc
     9361 tacttctgaa aataacatgt attccatatc ttgaacaaca ttgtcttttt ctattacttc
     9421 gtttatatct acttttccgt ctacattaga aatagtattt ttatcatctg caatatagat
     9481 tacattataa ttaaaactac ctctgcaaag tatcttccca tctgccattt caattttttt
     9541 aagtgatatg tatccttctg tttttacaat ttcatataca tctaattttt tgtctggtac
     9601 aactgcctcc gcttcaatga atgtttgaaa ttttccaaag tctattctat tgtcaacttt
     9661 aattacatct ttaattaatt ccatagtcta cctccttaag tattataaaa atatcacact
     9721 atatttatat ttaaggagca aaaaaaaaat tacacctatt tttaaatagg tataatttta
     9781 acttatttgt aatttatctt gatctctaaa aacttgcaac tttacattag atgttaataa
     9841 atctgagtaa ctataagata ctctaggata tccattactt tcatcatcta actttataac
     9901 aaacacgctt ggataaactt tttctagtat accctctttt gtaataatct gctttcttcc
     9961 tttattagct ttcaacaata ttttctttcc tatatgtctc tctaagctta ctcttatctt
    10021 atctagagtt tgaacagtag ccacaatatc accttcttta acgtttatat gtttacataa
    10081 tatcacatat aaacattttt gtcaaatgtt taaactaatt attttatata ttttttgcaa
    10141 aaatgtcaat actatttttg aagatataca gtataattat gttaattttt gctaatgtaa
    10201 tattgttttt ggcattgccc atctaaattt tcctctggtt tcagaaccac caattccctt
    10261 aacacctgta atagtctgct ctataacctc atcaatagga actactacat caattcttga
    10321 acaagcaatt ttctccacta taaaaacagg gtctaatgca tgtatcatta tccttccagg
    10381 cgaacttgca tagtttgctc ctgcacttat tattttttca taatttgatt ggcatgctcc
    10441 tgcgaatatg acaagattat ccaaatttgg ttgccattta cgtgcttctt ttactgtttt
    10501 aacaaaattt gaagagtttc tatagttact catatcttct atatctcctc tttttattgt
    10561 cattgcatca tgacctgtta ttactagtat atccggatta tatttttcta gtagtgctct
    10621 tacttcttta tactgatttt gctctgaaac agctacacct actgcaggta ttccaagttg
    10681 agcatataca tccaagcaaa tttttaaata ttctttatct ccatctatct gtagtacttt
    10741 tccaggcatt ccatatgtgt taggatttaa ttgaagtttt ggaactgctc tagtcattct
    10801 tttttgtctt tctttagctt ttcttactga cttatataat aaattttcta catttttatc
    10861 tattaatata tcttttatat caggtgcttt tactaattct aggtcatcaa tataagcatc
    10921 tgctattact ctaaatgcaa ttccctttag tatagctatt ttctcattat tttcatctac
    10981 accaaaagag actattttaa atactatatc tttgttatgt gactttctag ctacaatgtc
    11041 tcctaccttc atagttaaac ctccaataaa tttttatctt agtttatact atgttcgata
    11101 taaaactttg tttcctaatg tcaaaaaaac atttcttata atacttcttt tatcactctt
    11161 aaagcatttt tatagtatat cttttctata tcatcttcac taaacccttc tttttttaga
    11221 ggttcataca gtttacccat ctgagatata tcttcaatct caacttttga atctattcca
    11281 tcaaaatctg accctagaga tacaacatct attcctccaa catttacaat atgttttatg
    11341 tgtctaacca tatcttcaag tttactttca cttttatcac ttaaaaataa gtggaagaag
    11401 tttatacctg taactccacc tttatttgct aatactttaa tcatatcatc agttaaattt
    11461 ctagaatgat tcatcattgc tcttgaatta gaatgtgtag caattattgg tttagaagaa
    11521 atctttgcaa tttcataaaa tccaccatct gaaatatgag aaacatcaac caacattccc
    11581 aattcattca tcttatgtac cacttctctt ccaaaatcag taagcccttt ttctctatat
    11641 tctactttat tatgaggaaa gctcaattca ttcacatgat tccaacttat tgtcatcatt
    11701 ctaaccccta agtcgtaaaa tttctttaaa ttttctaatt taccttcaag tactgcacct
    11761 tcctcaatag ataaaagagc tgtaagttta ccttctgatt ggtttttcat aatttcttca
    11821 tagttagtag ctaaagctat ttcatcacta ttttttttca tttcctcatg aaatttattt
    11881 gccatactca tgcaataatc aaaaggatgt tttacttctt cagtatcaac aaaaagagca
    11941 aatgtttgag ctaatgaatc cccctttttc aatctgtcta tatcaacaga atatttatta
    12001 gacttaagtt cacttgtttc tacattttcc attaattttg ctatagtatc acaatgtaaa
    12061 tcaataaatt tcatatttat accccctgta tattttatat ttatatctaa aaatagaaat
    12121 agttgtcctt atagacaact attgacaact attttttaat attcttttta atattactta
    12181 atcttattct tcttttaagt taggtattac ttttttaact tcttcaattt gatgccatgt
    12241 tttatctata ttattttcac taatatattt ttttaaatca gttaaagatg taaaatcttt
    12301 aactgataaa ttgtcatcat catctgcaaa tcttaaaata aacatgagac tcccttactt
    12361 tccacaacat ttcttatatt tttttccgct tccacatggg catgaatcat ttcttcctat
    12421 ttttgcttct ttaactattg ttttagactt cttataagcc tttattattt cttttctttg
    12481 gtctgctggt agtatatctt cccaaccttg taaattatat agccaatgag cctcaacagt
    12541 taccatattg ttatataatt tttcccaatc tatttttata gaaacttctt tatcagcttc
    12601 tacttcttct aaaactatat cttcttttaa gctttcactt attccatcta tgaatcccat
    12661 gaaaaattca ttagtagttc caaatttttc agccaattca cttactttac cttcaacaac
    12721 atccatttta ttatttaata tttcattgta tattccagct tcaactttca aatattcttc
    12781 ccagaatttt acttctgctt cttgactttc atgattatca cttaaatttc tccattcagt
    12841 atataaactc atactttttc ctcctaagta tacctatatt tttatagttt ttatagtata
    12901 aaatctttta gttagattgg aagtttcttc tttttgatta taccatattc tatttaaagt
    12961 taactcttat attatatcat aattaaacaa ttcttttaat acttttatag catactcttg
    13021 gtttataaaa taagggcttg tcgcaattgt tattactttg gcttttttaa ttaagttttt
    13081 tatcttgtaa aatttgagtt caaatggtat atagtccata tcacttgaaa atatatccaa
    13141 atctatgtca agaacaaact ctgattctac ttctaaatca aatccatata gactatctat
    13201 tattattaat tctgaaaata tgttatattt taaagcaggt tttataaaac ttcccacatt
    13261 taaaacttca tttgtatatc taaatacatc ttttagatta tttacatcta catcatagtt
    13321 ctcaggttct ctagtatcct tatgttggtc tacatgcact aacttgcatc cttttgtaaa
    13381 ctcacccttt aacaaagact ttgcccaaaa ataaaatgca tggttatgat tgtcaaacac
    13441 atatatcttt ttatttccca ggacatattc gaccatattc tccaatcctt ttgcttttat
    13501 ttccttattt tcatctactt cattaaatac tacctcattg cctaacctta catcatttaa
    13561 tgtaccattt ataagttttg gaacgtatat gctcttattt tctcttttat catatgaaaa
    13621 tacattattt cctactggtt tttctatata aaatccacta tattcataat tttccataaa
    13681 actttatttc ctccattgtt atataaatat ataagttata attttaaaaa ttaaaatgca
    13741 cttattgtaa atcatatcat ctattataga aaacatctta tataactaaa tataatatct
    13801 tctctcaata tgtattatac aataagtgta aaatatattt atataaaata tactacattc
    13861 ctataacaaa accatcagct tcaattggaa gataactaac tcttctattc aactccacct
    13921 ttccaaaaaa tactacccaa tatggtctaa aaatactttc aattttaact atttcaatct
    13981 ctgggattcc tcccataaac ctatgagtta atctcatagc cattttattt gcctttgctt
    14041 ttactcttcg ttcgtcctta tcactatact gcacaatatt acaatccaca ttttttacaa
    14101 cttcatatgt aggaaaatct tgtactatac taccagaacc tgtagaacca ttcactaaaa
    14161 tttttaattc atttatatca ttagaatatc tcctattctt gaaaatggat ttcgttttgt
    14221 gaattttaag tgtgataatt ttaaactcta tataatgaag tctaacttca tgagcatatt
    14281 tgttctttaa tattagctta ctaaataaag aattttttcc acttttagct atctctatag
    14341 cttcttcttt tgtaattctt ataggtaacg ctcttatatc cataaaatca ttctctttct
    14401 tatttttttc ttgtcatttt taaaactcta tatatataat caaaaaatat tacaattaca
    14461 actgacataa ctccatatgg aattattttt ccaactataa tatcatttct agtcaatata
    14521 ccataaaata taaatcctgt tattaaaaag tacaataata ctagaaaacc gtctattcca
    14581 agccatttta tttctaaatc tttttttacc ttagacacta atttagacat agcagtttct
    14641 ccttttatag attattttgt gtatttaaat tttcacctat tctattacca atcatccatg
    14701 caataaatga aaaaggtata gctatcacaa gaggctctaa ctcaaagctc tgaactggaa
    14761 atattttaaa ataaccaaaa gtcactccac caacaatcat tcctaaaata ccacccatag
    14821 atgttcttct tccatttcca agaagcccac ctactatagg tacaaatgcc gaacccatcc
    14881 aaatacttcc tacaaatata catgcatcat atacattttc aaatctaaat gctacgctaa
    14941 gccccataac tgctgctaaa catacaccta ttctagtaaa ctttactaat tgtttctgag
    15001 ataactcttt ttttgtaatt cttccatata tatcatttgc taatgtagcc ccagctgcta
    15061 gataatagct atccaatgtt gatattatag aacctaaaat tcctattata aatattactc
    15121 ttattccttg tggtaatgta tctaaaacta aagttatata tccagcagct ggagccattt
    15181 ctggataagt agctcttact ataagtcctg tcatagttaa caccatgtca aaacatatcc
    15241 atagtgaaaa acaggttaat aaggctcttc ttccaacctt aggagaatct gctgctgaaa
    15301 atctttgata aaaagttggg tctgaataag ctccaaaaga atatacaatt aacatcaatg
    15361 ctttcatagc tgacataccc tttgtagggt gcataagctg tggtgcatct ttgagtgctt
    15421 ctgtaagacc ttgaaaccct cctatttgtt caaattgacc tggcataacc atagttaaac
    15481 caaataccat acaaaaaaac aataccatat ctgtaatggc aactcccata agcccaccaa
    15541 gtactgtaag tacaacgact actcctacca ttattaatcc tattaattct ttatctattt
    15601 tccatgctac gttacctata gttcccatag cagttatttc tccaagttgt gcacaatata
    15661 taaacatcaa aattgcccca attatagcaa catgcttgcc ataaaaactt tccagtaaat
    15721 caggaactgt tatattggtt cttatatgaa tccttggacc aacccacaat gctaaaggca
    15781 ttctcccaat atgtgcagct attgaccaaa ctgcccatgc agatattcca tatatagccg
    15841 catactctac actacctact gtaccaactc ctccatacca tgatgcaacc aaagttccta
    15901 ctattaagga ccatggtaaa cttcgatttc ctaggtaata actttccata tcttttacat
    15961 ttctagaaaa ataataacct attcctaata atataaatgc aaatataact ataatcccat
    16021 tttcccaaaa tgttaacata tctaatctcc acattcttat attttaattt ttaactttaa
    16081 tatctaaata tgaacaactc tacccatttc cataaaaata ggtagagtta tataataatt
    16141 ataagtattt aagtaatctt gttattaagc aattttattg tttaaacctt tattttctct
    16201 tttatttatt ttatttaaag cataagtagc aaatatccct ataactgcac atactacaga
    16261 taaaataaat atatatgtat atcctgtttg tccatattta tctaaccaat tacctgcaat
    16321 agtgtaataa aacgcatctg gagaaaatcc tataaatgaa gcaaatccaa ctactgctcc
    16381 tgtagtttct aaagatatat tagactctga aacagatgca aagtatatac ctctaaatcc
    16441 aaataaaatt acacttagta ttatcatgtt tattacagct atccatataa atcctgtact
    16501 cttaggaata accaagtaag cagctgtact tactgtcatt aatataaatc ctacaaacat
    16561 tactcttata gatgaaccaa ctttatctgt tataacacca gcaactggag atgctcccat
    16621 ttttataaca taagttctta ttattgataa agcagaaact agagtcattg gcattacata
    16681 aacttctgat aagtatggac ttaaataagt taaacttgag aatactaaat aagttgaaaa
    16741 tattataaga ccaattagcc aagcctttgg cattgtaact acttttaaca atgacttaat
    16801 acttaatcct tcatctgatg ctccactatc ttttttctct tccactaaga aaaatagaag
    16861 tataccacat attatagatg ctgcagaata taaccatact acatatttga atcctactgt
    16921 tatttcagca aatttaccaa agaagtaaag acctataaaa gaaattaatg tacctgatac
    16981 acctgataac ccttcataaa aaccaaatat tcttccttgc tctgactcac ttccttgcat
    17041 tctaacaacc ttaacagagg ctgagaagaa agttaatatt gttgtaaatc cccataatac
    17101 aaatattaaa agtatcatac tatatgatgg gaatgtagag aaataaaatc caagtattcc
    17161 tgaagcaatt aaagaaaaag ttattaattt tctaactgag aatctatctg caaaccatcc
    17221 tcctataaaa taagatacca ttcctactaa tccatataaa ctcaataagt tccctatttg
    17281 tgtatgactt aatgctaaag cttgttgcat tgggtcataa aatgatgatt tcatatatgg
    17341 taaactatac atagctgttg ttccaaatgc caatatgaat attatcaaaa atcttttaac
    17401 attatttttg ctttccattg attctctctc cttaaaattt tattgtttat aatctcattc
    17461 atataatttt attttaattt ttctattgct tgtttaaaat catttattat atcatttgaa
    17521 gattctaatc ctacagctat tcttattaga ttttccccat tcatttgaac taatgtatgc
    17581 tcttccccta agcttgttgc tattgttgca agctctaaag aattcattaa tcttctacta
    17641 gcttggtctc ttgtcaatcc attgatacca tccttaagtt taaatgaaac tacacctcca
    17701 cctaaaccat tcatttgagt tttacaaagc tcatattgag gatgagattt taatgctgga
    17761 tactttacca attccacatt ctcttcttcc tctaaaaatt ctgctactgc taatccattt
    17821 tcacaatgct ttttcatcct catatctaac gttttcatac cccttaaaat catccatgca
    17881 ctcattggtg gtaaagatgc tcctgtaaaa caacataaac ttggccatct aactaaatct
    17941 attaattctt ttgaacctgc tactgctccc cctaaagtat ccccatgtcc atttataaac
    18001 ttagtaagac tatgtattac taaatctgca cctaaatcta ttggtctttg tacaattggt
    18061 gtcccaaaag tactatcaac tatagctaag cttttatttt catgagcaat ttctgctatt
    18121 gtttttatat ctactaagtc taatgatgga tttaaaggac tttctatata aactatttta
    18181 gtatttggtc tcattgcttc ttttacattt tgtggattta ctgcatctac aaatgtaact
    18241 tcaactccaa atttagataa caactcagac atgaataatt tagtgtgtga aaatattcca
    18301 tctgaactaa ctatatggtc accacttttt accaatgcta atatagtaga tgttattgcc
    18361 cccatccctg atgtactaga tatggcactt tctgcatttt caagcatagc caacttagct
    18421 tctaactctg taagagtagg attacactct cttgtataca catatcccca tgtcatacac
    18481 aactcttcaa aatgctctac tgtctttgca gcaaatgttg ctgtttgaca tattggtgta
    18541 gccaatgcac ctgttgttgg gtctgcacta tgtccccaat gtattatttt tgtctctaaa
    18601 tcttgattaa tattgttact cattttgatt tcctcccatt ttgacttatt tatacaacgt
    18661 tttccctacc aaatttcatc aatttagctt agtcttaata cattttttca agtatacttt
    18721 tttatttgtt ataatttcta aaaatatgac acttattttg tttttttaca gttctaatta
    18781 taaactctat agtaactata gagtcaatat gtttttgata ttttttttac aatgtttaaa
    18841 atagtcagta caaaaatagg tatatcttag aataactaaa gtcatcctaa gatataccta
    18901 tttttatatc acaatataaa taattacata taagcattag tttttttatc ataaatagat
    18961 ttttcaagat ttatcatctt ttcataaata tattatagtt aaatatttta atattaccta
    19021 acatctacaa taaaatatac tcttaaaata aaattatata attaaaaata ctctataaaa
    19081 gctaaaaagt ttttctattc gctacatcta aaaataatta gatagaggta taaatgtatt
    19141 tagtttattt atacctctag tcttttataa attatatctc tactaaattg attttttagc
    19201 acgaataagt gtgctcttgg agataccagt catcttcaca acctctctat atggcttaga
    19261 atttaataac cccaatgcat attgtatctg ttgattgctg tatttctttg gcctaccatc
    19321 tttaaagtcc tttttctttt tagcaaattc ttttcctaat tgagtacgtt ctattatcat
    19381 atttctctca agttctgcaa ctgcaagtaa tgtagtcaaa aagaatttac ccattgaagt
    19441 attttctaaa aggcctatat ttaaaatatg aacacttact tctttagcaa ataaaccttc
    19501 tattagttgt attccttctg atgtacttct agctagtcta tccagcttag ttgcaacaag
    19561 taagtctccc ttttttagag atgatataat tttttcaaat ttaggcctgt ttgtagtagt
    19621 gcctgtataa gtttccttta ttattttagc atcattatat ttattcaaaa tttcttgctg
    19681 ttgctgatac aaactgttgt tgtctgtttg attttttgta cttacacggc aatatccata
    19741 tataataatc atcacttcct aacataaaga ttttgtacag aagttaccca gaagcttgtt
    19801 tttacttatc tttatccatt ttactaaaac gatttttggg tgtcaatact gttaactttt
    19861 gacaccataa taattccttt ataatttgtt tgactatgtc aaaaattacc aatataatga
    19921 aattaatgag tttatacaaa ttggaggaaa taatatgaat agtctgttaa aagaagcaat
    19981 agatgaatta aaaaacatcc attgcgggga agttttttta ttaaaagaat tgttcaaagg
    20041 ctatgaatgg aacaggttgg agatgaatat cagattgaaa ttaggaaaac tttttttgta
    20101 tgaagtggta actaatccca atttaaatgt agaggtcatc aataaaaatt ctgcttatca
    20161 gcaaatgtat aaaaaaaaat agtgacttaa atgtatataa tagtataatc ttcttattga
    20221 ttcaaaagtt aaaactttta atacaaaaca atcattctat tttaacattt atcaatatgt
    20281 ctaaatttat ataattaaca catacgaata gtattgttta aatcacatta tttaagatag
    20341 ataaatctta accctactcc agcataaata ctaaaatagg gcttagaatt atctatattt
    20401 ttatcccata aaatgcttag ctcttcctct tgattgaaca tatatatctg gggaaataca
    20461 atagaagcgt aaatttataa aatttccaaa gtcagaaaac ttcagttcaa acttaggtat
    20521 accaaaactt tcaggtgcta tttcgtcaac gccaaaatag atgataattc cattatctgt
    20581 aagataaaat gcttggtctt caggtataac taactcaaca ttagaataat acatttctct
    20641 attttcattt atttgagatt taatgtaatc agtaattata ttaatataat caactccatc
    20701 tctaaaaatg tcattaagta aaagttcttt tccagttgtc aaatctatat tgtaattatt
    20761 caatgtattg tatttaggcc catagttatt agcaaacccc ataaggttta atatcatact
    20821 cagtacataa tttttattaa aagtaactgc gtaactggta gatacccaat agctttgttt
    20881 tggaagtgaa ttttgagaag ttgcgttatt atattctcgt tcttcttcct gtaagccatt
    20941 tttaaaatta tttacagtca ttcttacagc attgtttatg ttatttaaaa taactgggtt
    21001 tacatacgtg gattgatttc taatattgta tataacagga tactttacat catatgaaaa
    21061 atacttactt ctttcaaaat cttcattaaa ttgtacatat aatatattat atgtacaggt
    21121 atttgtagat tgataatttt ccattaaaat cctccccctt ttataaaaaa atcagataca
    21181 atataatatg cttaaaacag taataatgtt aatggtaaat gtaaagaata gaaagtatct
    21241 aaaacagcaa aaaatgacta attttgtggt ataatacata ggattgagag ttattttata
    21301 ggaggaaaaa tatgttatta ttagcagata aatggaaaga ttatgaactt atagatatgg
    21361 gaaatggaga aaaattagaa cgttggggaa gttatgtact tagaagacca gacccacagg
    21421 ttgtatggcc aatggaatct gaatgggctt tatggaaaaa ccctcatgga cattatcaca
    21481 gaagcaataa aggtggaggt caatgggaac acaaaaaaag atatccagaa caatggacta
    21541 taaattataa aaacttaaag tttcacatta aaccaactgg ctttaaacat actggattat
    21601 tcccagagca agcagctaac tgggattgga tgatagagaa aataaagaaa gctaacagac
    21661 ctataaaagt gttgaatctt tttgcatata caggtggagc tactgtagca tgtgcatctg
    21721 ctggtgcaga agtatgtcat gttgatgctg caaaaggtat ggttaattgg gcaaaagaaa
    21781 atatagaatt atcaggtctt aaaaatcaaa cagtaagatt tatagtggat gatgttgtaa
    21841 agtttgttga aagagaaata agaagaggta aaaaatatga tgcaataata atggaccctc
    21901 cttcttatgg aagaggtcct aaaggagaag tatggcaaat agaagaaaag ttatacagtt
    21961 ttgtagattt atgtatggga gttttatcag ataacccttt atttttctta attaattctt
    22021 atacaacagg attttctcca atagttcttg aaaatgtatt gtctacaaca ataggtaaaa
    22081 agacaaatac aggagatata tacggtgggg aattaggaat accagcttcc agagatggta
    22141 agatacttcc ttgtggtata tttggaagat gggagaaaaa ataatgataa aagtcatata
    22201 tgaagataac catttattag ttgtagaaaa accagttaac atattgtctc aaggagataa
    22261 tactaatgat aaagatatgg taaatctttt aaaagactat attaaaaaaa agtataacaa
    22321 acctggaaat gtatatattg gtttaataca tagattggat agaccagtag gtggagttat
    22381 ggtgtttgca aaaacatcaa aagcagcatc tagattgtct gagcaggtaa gaagtaagac
    22441 tttcaaaaaa acttatctag ctgttgttca tggcaagata aaatctaact cagatatact
    22501 aaaagatttt ttatataaaa ataaaaaaac taatatggta actgtagtga aaaaaagcca
    22561 taaagatgcc aaagaagctg aacttaacta taatgtatta gattttaaag aaaatctaag
    22621 tttagttcaa gttaatttaa agactggtcg ttctcatcaa ataagagttc aatttgcaag
    22681 cagaaaacat ccattatacg gagaccaaag atatggcaaa gaaataaata agattggaca
    22741 acaaatatct ctttggtcca ataagataga aatttgccat cctactacaa aagaaaaaat
    22801 ggaatttaca tgtcatccac ctgacaaata tccatggaat atattttaaa tctcaaatga
    22861 ggtgattata tgagtaaaaa attttcaata ggagaaatgt ctaaacttca taaaataagc
    22921 atacagacac taaggtacta tgaccaaata gatttattta aacctgttca tgttgaccaa
    22981 gaaagcaatt acagatatta ctcaattgac caattttcac aattagatat tataaaatat
    23041 ttaaaatatt taggtatgcc tttaaaagaa attaagtcaa agcttgatga aaataatgaa
    23101 gaaattctaa aattactaac aaataaaatt aatatagtgg atgaaaaaat taaagaatta
    23161 gagcttataa agaaggtact aagcacaaag aaagaaacta tggaaaaagg tatgaaagta
    23221 gatgaagttg gtaaaatagt gagaaaacat attccacgaa gaagtatctt atctatagag
    23281 tatgaagaag gattaagtag tgaagaaact atggaacttt ccagaagaca aatagcaaat
    23341 atattagaag ataatatatc tgtgttctat ggtggagtaa gtggacttgt atctatagat
    23401 aaatttttaa aagaagataa agttatttat gacaatagtt ttgttactgt tgaaagagac
    23461 ttatttgata taaaatcaca aaaacatata acagaaattt catctggtga ttttatttgt
    23521 atgacatata aagggcctta caaagaaaat tataccgctt ttaagaaact ggttgattat
    23581 gttgaatata aaagaatacc tattgaaaat tatatatatg aaatacctat aatagaccct
    23641 ctgtctacaa acaacgaaga ggaacttcta acagaaattc aaatacaagt taaaaagaga
    23701 gaaattgact taagatgtct agtgtagtaa tctagtttac ttacacatct taatatagtt
    23761 gtctattatc aacatatgat ttttctagat agtgattcct aacagtgaga ttttcaaaaa
    23821 ataggaggaa atatggctaa tgctaattcc ctcctatttt taattaagcc atatcatttt
    23881 aattaagcca tgtcatcacg aagtgcatca ataatatttt ctttttttat cttattaata
    23941 gtatacaaca ttgtaataaa tactataaat aaaacactaa atatactaat tccaatacta
    24001 aaccatggaa tcacaaaatc aatatcatct gtaaccatta ctttatgaat tagccatgaa
    24061 gaaattactg ctataggaag tccaaagaac aatgctctca tgccataaaa ggcacactca
    24121 aaattcatca tcttttggaa atcatgctca gacattccta cagagcgaag catggcaagt
    24181 tctctacgac gtaacttaat attagttgaa attgtattaa acacattggc aatcgaaatc
    24241 agagaaatca taattatgaa agtataagca aaaacattgg caataaaaat gtaattacgg
    24301 ttctcatcca tcattccata catattgtac agattgtact cattcgtaac tccctcactc
    24361 tcaataattt gctccatttc tgcaactgac tgagtaggat ttttcgattg aaagcttaaa
    24421 ctttcaattc ctgtagaaac accaggaatc tcaaacttat ctttggctga ataaggagcc
    24481 aatactctga agcttgcttt cttcacatct gaacttctga ttcctggagg agaatcaggt
    24541 ggcactactt tgacaaatga aatattcaca ttttgtctct caggtatctc caacttgcca
    24601 ttagtttcag gagtgaggct aacattcata gaagaactgt tgaaaatatc aataatatta
    24661 ttatcctttg ttaccataga aacagcaatc agttttgcat tttgtccagt atattcttct
    24721 gctggtaatc caagactttt aataatattc agataagttc tatcatcaag aaattgcata
    24781 tctactggta attcaagcat ttcactcata gaatgtgaac cctcatagtt caaataatcg
    24841 tctgtaagct cacttacttt ggcaacacat gaataggtca taagtttctg atacaaacct
    24901 tcgtaaacac catttgcagt ttttagctta tcgtaaagct tcaacatttc gttgtctttc
    24961 ataccttgtg tagaaaaaat aatatcctgg tcagtaacct ctgacctctg atttgacatc
    25021 tgttttaggt ctgctactaa agcacttgta gatataaata gaactatgct taaaacaagc
    25081 gacaatacaa tacttcgata acgtttcttg tttcttttaa agtttttcat agcaagaact
    25141 ccctcaagac cataaactcg ctgtgttagt tttgatgtct tcacagattt agattcaatt
    25201 tttacctcat ttgtctgacg aatacactcc atcactggtg tgttggcagc ctttcttgct
    25261 ggtatgtaag cagaaattaa gattgtaacc atacttacca ctgctgcaac gatgattgca
    25321 gggatagaca atgttaatgt caacggaacg tttacataga gaatatttgc aaagttttta
    25381 gctatgacag aaatcacaag tccaatactg actataccga ttgcaacacc aattggtata
    25441 ccaatcgcac caatgcaaat tccttcaaat agcaccgaat ttcgtaactg ttttgctgta
    25501 gctccaacag atgaaagtat tccaaattga tgtgttcgct cgttcaatga catgttaaat
    25561 gaattatata ttagaaaaat tgagcctagc atgattatgg caagcacaat gccaccaacc
    25621 gagtacaaaa acatattgaa caaattgtta tcagaaacac ctatgaagcg caacacattg
    25681 tcattgaaaa tggaagcatg gtctttagtg ctacttatgt aagagcgaat ctgatgtgga
    25741 tttttcaatg tgacaaaaag gctaagactg tcagtcttga cttgtttatc ttctttggtt
    25801 atcaaggtat atcctggtgc agaagattct tcaaatacgg gctttctaca gatacccaca
    25861 actgtataag ttttcttatc ctgtggcaca aaaacttcac tcccagcagt gtatgggtca
    25921 ctctgaccaa gcttttcatt ttcactcatt cgtttcccta catccaaaga aatagtatca
    25981 cctactgcaa atttgacacc accatctgtc gcaactttac ttgaaacaag aatctctcta
    26041 ctattttcag gtaatcttcc agaaattaaa gttataggca aagtatcaaa agcttctttg
    26101 ctaaatccag caataaaaag atatggttta tctggatttc tgttaccttc aagtgttgca
    26161 tagccaatat tttcaaatgt tgcagtattt gcaacttcat cattgactct ttgttcctgt
    26221 acaaaagaag aatccacatc caaaaactgg acatgccaat caccatattt tataatagct
    26281 ccatttgtca tatagttcat caaagaaact ccaaaggtag taactgctgt aatcatagca
    26341 gctgacagaa caactcctat aattgttaca agagttcgag tgcgattctt tttcaatcct
    26401 tgcagagcaa ctttgttaaa aatgttcatt aacgattcac cttcctctca tcacgcacta
    26461 cttttccatc tgaaatacag ataattctat ctgcctgtaa agcaatgttt tcatcatgtg
    26521 tgacaataat gagagtttgg tgatatttac tatggctctc tttcagtaac tggataatct
    26581 cttgtccatt tttactgtct aagctacctg taggctcatc agcaagcata actgctggtg
    26641 cattcatcaa tgcacgacca atggcaacac gttgctgttg gccaccagaa agttgatttg
    26701 gtaaatgtgt ttttcgttct tttaaaccaa gcaattctaa caagtcattt aaccgttctt
    26761 gattgacctt acgcttatcc ataagtatag gcaatgtgat attttctact acatttaatg
    26821 tgggtatcag attgtgaaac tgataaatca atccaacttg tcgcctacga aaaatagcaa
    26881 gtttttcatt gctttggcca tatacatctt gcccctccag atatatcttt ccacttgttg
    26941 gtacgtctac tcctgcaatg gaatgtagta gtgtagattt accagaacca gaagaaccaa
    27001 tgattgcagt aaactctcct ttttcaatag taagtgagac atggtcaagt gcagtaacct
    27061 tattttcatt cttaccataa accttacata aatcttcaat ctttaaaaac gccattatat
    27121 aagtctcctt tcatatttta taaatatatc ataggacttt caacttacat ttaagtgaca
    27181 tttaaatgag agattcgtca ctttggaaaa cgaataacaa atattgcacc accttgtggg
    27241 tgattttgtg cagtaattgt tcctccttgt ctcataataa tcgtcttaca aagtgccaat
    27301 cctattccat aaccggctgt gcttgaattt ttcccacgat aaaatctgtt aaatagacat
    27361 ggtaaatctt cttttttaaa gcctgctccg ttgtcatgaa tagtaagttg agtaaacaaa
    27421 aagttgtcct cacaaatgat atcaattttt ccattatcac ccacactctc catgcagttt
    27481 ttaaatatat tttgaattgc ttctgaaagc caatttaaat cacccaaaat ccttactcct
    27541 tttggtatat caatatgtaa agtgatattg tgcagttcca tgggaatcaa aagttgatga
    27601 agtgcagaac taatcaaatt attcacatca atttgttctt tttgaaagac tacaatacct
    27661 gcatctaaac gtgataattt taaaagggaa gtaatcagcc agtccattcg tataagtaat
    27721 tcctctgttt ctcgtatcaa ctcttttctc tcatcttcat cagaagtatt ctctaacagt
    27781 gacaaaataa gagttactga tgtaagagga gtgcgaagct ggtgggctat gtcagctagt
    27841 gagtcagcaa ggtgttcttt ttcttttttc aatgcgtaat tttgctctct aatacgtaac
    27901 gtcattttag ttatttcact ttgcagaata gaaagttcac cctcatctga ttcagcaata
    27961 tacagatggt tagcattatg aagcacaaga ttaatttgtt ctgaaatctg tgcaatactt
    28021 ttgtatcgat ttttagtaaa cacaaaaaat gctattccaa aggtagtggc agaaacaata
    28081 gaaagtattc cagtcataat actgattgca aatcccaggg ctacagtgag aatagtaatt
    28141 aataagaata aaatagcaaa ttttcgaaac tctctatttc gaaacataac ttccccccaa
    28201 tctataccct gtcccacgaa cagtcaaaat tatttgtgga cttgaagggt catcttctat
    28261 cttctctcgc aagcgtttga tgtacacagt caatgtgttg tcattaacaa actcacctgc
    28321 cgcatcccac aattcatcaa gtaatcttac cctcgtaata atacttttag gattgctaat
    28381 aaacaccaat aacaaacggt attctaaagc tgaaagaaaa acctcattgc cattcttttt
    28441 cacaacaccg gttgccgtat ctacataaag cccacaaatt tcaaaagttg acccaaaacg
    28501 cccacttttt cgtagtgctg ttctaattcg cgcaatcagt tcacgtggac gaaaaggttt
    28561 agtaatataa tcatctgcac ccatattcag tccagtaaca acacttgatt catcaccgga
    28621 agctgtcaaa aagataacag gaatgtcttg cttttctttg atttctgtgc aaatcgtaaa
    28681 cccatttcca tcaggtaaag aaatatcaat aagtgccaaa tcaaatttat tcttagcaat
    28741 ctctacaagt gcttcacttc ttgtggatac atgggttact ataaatcctt ctgaattaag
    28801 caaaagaata agatttttgg caattgcttt atcatcctca actaaaaata tccgtttcat
    28861 ttactttcat cctcctttat tttattagtt ttatatattc cagcagagca atagtacact
    28921 actattttac ttatcattac acaattttca gtagtttctt ttgttcttat ataatccatg
    28981 cataatacct ccattgattt attatatttc ttataacata aatacacaag ataagacaaa
    29041 aaacataatg taagtgcaag ttttttaact cactaactta taaacataca ctcctactaa
    29101 cactactttc ttgatgacac atcttacaag cttatattca tacatcaatg aaaaattaaa
    29161 tttatccatt tattaaaata aaaggcaata gcttctatag ctaatgccct ttatcttaaa
    29221 atgatttaaa tactatacaa tttttataaa tggctataaa taaactcgta tagaatataa
    29281 aatttatcta cagtcttttg ttatacaaaa tttataacct cttatagaac aaatttacca
    29341 ctttgcaaac ttatcacttt tactcagcta tattataaac ttttaattta tctttgttta
    29401 aatgtttctt cttaacttct actaaagctt ttagcgtatc caaagatgtc attatttcag
    29461 tagaaaattc aatggctgtt cttctcatct taaatccatc tcttgtagag tcatttcctt
    29521 tagttggtgt atttattact atatcaactt gtttatttct tatagcatct aatatgtttg
    29581 gtctaggctc ttcaattcta tttactatat cagcctctat tccattttct cttaaactct
    29641 tcgctgttcc ttctgtagca acaaaagtat atcctaattc tttcatatct tttgctatct
    29701 ctataaactc atctttatca tgattattta cagtagcaag tactactcct ctttcatcag
    29761 acatatgtct acctgctgcc aagaaccctt tatataaggc ttcttctaaa ttttctccta
    29821 cacctagaac ttctcctgta gacttcattt caggtcctag acttacttca actttagata
    29881 atttagacat tgagaatact ggtactttta ctgatactaa ttttggctct ttgtaaactc
    29941 ctgtactata tcctaaatcc tttaatttag ctcctaacat acacttagta gccaagtcaa
    30001 ctattggtac tccacttact ttacttatat atggaactgt tctactagct ctagggttaa
    30061 cctcaattat atataattca ttttgaaatt ctataaattg tatattaacc ataccaagca
    30121 cattaaggtc taaagctatt tttttagtgt attctactat tttttctttt atttcatcag
    30181 atatattttg acttggatac attgttgtac tatctccaga gtgaactcca gctctttcta
    30241 aatgctccat tataccaggt atcaatatat cttctttatc acatatagca tctacttcta
    30301 tttctctacc agttagatat ttatctataa gtactggatt tttatggtct ctatcaaatg
    30361 catcatccaa atactgaatt agtttttctt cgttataagt tatttccata ccttgaccac
    30421 caagtacata agatggtcta actaatactg gataacctaa ctccttagca atttcaacac
    30481 cttcattagt tgtccataca ccttttcctt ttggtctatt tatatctagt ctctctagta
    30541 aatcatcaaa tttttctcta tcttctgctg catctatatc tctaaagtct gttcccagta
    30601 taggtatatt tttttcatgt aaaaacttag ccaattttat agctgtttgt ccaccaaact
    30661 gtagtattac accttctggt ttttcttttt ctattatatt taaaacttct tcttctgtta
    30721 atggttcaaa atacaactta tcagatgtat caaaatccgt acttacagtt tcagggttat
    30781 tgtttacaat tatagtttct attcccattt ttcttaaaga ttttacacag tgaactgaac
    30841 aatagtcaaa ttctattcct tgacctattc ttattggacc agaacccaat actacaactt
    30901 ttctcttatc actaactact acctcatcat attgttcata agttgaatag taataaggtg
    30961 atagagcttc aaattctcct ccacatgtat caaccatttt ataaactggt tgtatattat
    31021 ataaacttct caattcatat aatttttctg ggcttatctt cattaagtca gatatacctt
    31081 tatcagaaaa tccctttttc tttaattcat gtaaatattc tttatctaag tcttctattt
    31141 ttagaacttt taacttttct tcttgttcta ctatccattt aaatttattt ataaaccact
    31201 tatcaacacc agttatttct gatatcattt ctactttata gcctcttctt atcatttcag
    31261 ctaagtcaaa tagtctctca tcatcaggta ctactactct agcctttaat tcttctaatg
    31321 ttctaccttc tgatggagca tgaaccaatg aatattttcc tgtttcaagt gatcttatac
    31381 ctttaagaat agcagcctca aagttacttc ctatactcat gatttcacca gttgccatca
    31441 tcttagttcc taattttcta ttggcatgtc taaatttatc aaatggccac tttggtattt
    31501 ttactaccac ataatctaat gttggctcaa aacaagcata agttttttta gttactgcat
    31561 tttctatctc atccaaagta taacctaaag ctatcttagc tgctactttt gctattgggt
    31621 atcctgtagc ttttgatgct agggctgatg acctactaac tcttggattt atttctatta
    31681 tcgcatattc taaactatgt gggtttaaag ctatttgaac gttacatcca ccttgaacct
    31741 ctattgcatt tattatgtct attgatgcct ttctaagtag ttgatactct tcatcactta
    31801 gagtttgact tggagcaaca actatactgt ctcccgtatg aactccaact gggtctacat
    31861 tttccatatt acatacagtt atacagtttc catttccatc cctcattact tcgtattcta
    31921 tttctttcca accttttata ctcttttcaa gaagaacttg tccaacagga cttagctgta
    31981 gtccatgtga aagtatttct cttaattctt cctcagtatc agctatacct ccaccagttc
    32041 cacctagcgt atatgctggt cttacaacta ctggatatcc tatcttattg gcaaactcaa
    32101 gacctgcttc taaatttgta actatatcac taactattac tggttgattt atttctttca
    32161 ttacttctct aaataaatct ctatcttcgc ccttttttat agattctata gatgttccta
    32221 taacttttac attatattta tctaatatac cagcatcatg taattctaca gctaagttta
    32281 atcctgtctg accacccata cctgcaagta aactatctgg tttttctttt tctattattt
    32341 tttctataaa ttctattgtt aatggttcta tatatatttt atctgcaatt tctttatcag
    32401 tcattatagt agctgggtta ctatttacta atacaacttc aattccctct tcttttaatg
    32461 cttggcaggc ctgtgttcct gaatagtcaa actctgcagc ttgacctatt attattggcc
    32521 ctgaccctaa tactaaagtt ttctttatac tatctaattt aggcataact ctttctcctt
    32581 atctaaacaa ttttcacagt tcttatcttc atcatctata caatcaactt taattacttt
    32641 attttatctt aaattcaaat ttactcatca atacatctat ttaatacatc tatgttaatt
    32701 aaaccaaact ttttgattta agttattctt gttttttagt tagctattct aatgttattt
    32761 attttactaa ttctaaaaat ttatcaaaaa tatagtcatt atcttttggt cctgggcaag
    32821 cttcaggatg gtattgaaca ctgtatacat ctagtttctc atgtctcatt ccttcaacag
    32881 tattatcatt taaatttata tgtgtcactt tcatttgttc aggaacttct gaaacataat
    32941 aaccatgatt ttgagaagtt ataaatactt ttccttcttc taaatcttta actggatgat
    33001 ttccacctct atgcccaaat ttaagtttag cagtttttcc tccaagagct aaagctaaaa
    33061 gttgatgtcc taagcatatt ccaactatag gttttttacc aataagagct tttatatttt
    33121 ctataacatc ttctaggtct tctgggtctc caggtccatt tgacaagaat attaagtcag
    33181 ggtttatact caatacatct tctggagatg ttgtagctgg aaatattgtt atatcacatc
    33241 cacgagctat aaatgatctt aatatatttc tttttactcc aaaatccata acagccactt
    33301 ttggaccatt tccctttata tgctctactt cttttcttgt aacagttcta actgcttctg
    33361 tatttgaaaa cttatccaat ttatgtttaa catcttctaa tttactgtct tctaatgtaa
    33421 ttataccttt catggttcca ttatttctaa gtatcttagt caaagctctt gtatctattc
    33481 cttcaagtcc gataacttta ttttgcttta aatattggtc tatatcaatt tcacatctaa
    33541 aattacttgg agaatcactt ttctccctta ctatgaatcc ctttacttga actttttcag
    33601 actctgctga tgataaattt ataccataat ttcctacaag aggataagtc atagtaacta
    33661 tttgaccata gtaagaaggg tctgttaaaa cttcaccata tcctatcatt gaagtattaa
    33721 atactacttc tcctacactt tcttctaaat atccaaatgc ctttcctata aaaactgttc
    33781 catcttctaa tattaacctt gctttcatac tacagtacct cctataattc tctttagaca
    33841 aactaaagaa cattaactaa atataagctt ttaaatatct atatctattt ttcactttta
    33901 cattctagtt ttatacttat ttacatttat tttttatctt tttctattat actacaaatt
    33961 tcttaaaaaa aagaagttat ctcaaaagta aaaactccta gaaaaaataa cctctttttt
    34021 ttatttaata attaaattct taaactatac aatcaactta atttctattc agctatattg
    34081 taaactttta actcatcttt atttaaatgt ttcttcttaa cttctactaa agcttttagt
    34141 gtatccaaag atgtcattat ttcagtagaa aattcaatag ctgttcttct tatcttaaat
    34201 ccatctcttg tagagtcatt tcctttagtt ggtgtattta ttactatatc aacttgttta
    34261 tttcttatag catctaatat gtttggtcta gattcttcta ctctatttac tatatcagcc
    34321 tctattccat tttctcttaa gctattagct gttccttctg tagcaacaaa agtatatcct
    34381 aattctttca tatcttttgc tatctctata aactcatctt tatcatgatt atttacagta
    34441 gcaagtacta ctcctctttc atcagacata tgtctacctg ctgccaagaa ccctttatat
    34501 aaggcttctt ctaaattttc tcctacacct aggacttctc ctgtagactt catttcaggt
    34561 cctagactta cttcaacttt agataattta gacattgaga atactggtac ttttactgat
    34621 actaattttg gctctttgta aactcctgta ccatatccta aatcctttaa tttagctcct
    34681 aacatacact tagtagccaa gtcaactatt ggtactccac ttactttact tatatacgga
    34741 actgttctac tagctctagg gttaacctca attatatata attcattttg aaattctata
    34801 aactgtatat taaccatacc aagcacatta aggtctaaag ctattttttt agtgtattct
    34861 actatctttt cttttatttc atcagatata ttttgacttg gatacattgt tgtactatct
    34921 ccagagtgaa ctccagctct ttctaaatgt tccattatac caggtatcaa tatatcttcc
    34981 ttatcacata tagcatctac ttctatttct ctaccagtta gatatttatc tataagtact
    35041 ggatttttat ggtctctatc aaatgcatct tgtaggtatt gagataattt ttcttcgtta
    35101 taagttattt ccataccttg accaccaagt acataagatg gtctaactaa tactggataa
    35161 cctaactcat tagcaacttc aataccttcg ttaagtgacc atacaccctt tccttttggt
    35221 ctatttatat ctagtctctc tagtaaatca tcaaattttt ctctatcttc tgctgcatct
    35281 atatcatcaa aatcagttcc cagtataggt atattttttt catgtaaaaa tttagccaat
    35341 tttatagctg tttgtccacc aaactgtagt attacacctt ctggtttttc tttttctatt
    35401 atacttaaaa cttcttcttc tgttaatggt tcaaaataca gcttatctga tgtatcaaag
    35461 tctgtactta cagtttcagg gttattgttt atgattatag tttctatgtc catttttctt
    35521 aaagatttta cacagtgaac tgaacaatag tcaaattcta taccttgacc tattcttatt
    35581 ggaccagaac ctaatactat cacctttttc ttatcactaa caactacctc atcatattgc
    35641 tcataagttg aataatagta aggagatata gcatctatct cacctgcaca agtatcaacc
    35701 attttatatg ctggtttgat attgtataga cttctcaatt catataattt ttctggactt
    35761 atcttcatta ggtcagatat acctttatca gagaatccct ttttctttaa ttcaagaaga
    35821 tagtctttag ttaaatcttc aattttcata acttttaatt tttcttcttg ctctactatc
    35881 catttaaatt tatttataaa ccatttatct atacctgtaa tttgttctat catttctact
    35941 ttatagcctc ttcttatcat ttcagctaaa tcaaatagtc tctcatcatc aggtactaca
    36001 actcttttct ttaattcctc aatgcttctc tcctcagatg gtgtgtgaac taatgaatat
    36061 tttcctgttt caagtgatct tatcccttta agaatagcag cctcaaaatt acttcctata
    36121 ctcataattt caccagttgc catcatctta gttcctaatt ttctatttgc ttttttaaat
    36181 ttatcaaaag gccattttgg tatttttgcc accacataat ctatagttgg ttcaaaacaa
    36241 gcataagttt ttttagttac tgcattttct atctcatcta aagtataacc taaagctatt
    36301 tttgatgaaa ctttagctat tggatatcct gtagcttttg atgctaaagc tgatgaccta
    36361 ctaactcttg gatttatctc tataactgca tattcaaaac tatgtggatt aagtgcaaat
    36421 tgaacgttac aaccaccttg aacttctact gcatttaata tatctaaaga tgctttttta
    36481 agcattgcac attctttgtt acttaaagtt tgagttggag caacaactat actgtctcct
    36541 gtatgaactc cgactgggtc tatattttcc atgttacata caactataca atttcccttt
    36601 gaatctctta ttacctcata ttctatttct ttccaacctt ttatactctt ttcaattaat
    36661 acttgtccaa caggacttaa ttgtagacca tgtgaaagta tttctcttaa ttcttcctca
    36721 ttatcagcta tacccccacc agttccacct agtgtgtatg ctggtcttac aactactgga
    36781 taaccaattg taagtgcata atcaagacct gcttgtaaat ctgtaactat atcactaact
    36841 attacaggct gatttatttc tttcattact tctctaaata tatctctatc ttcacctttt
    36901 ttgatagatt ctactgaagt acctattatt tttactccat atttatctag tatgcctttt
    36961 tcataaagtt caactgctag gtttagccct gtttgtccac ccatacctgc aagtaaactg
    37021 tctggtcttt ctttttctat tattttttct ataaattcta ttgttaatgg ttctatatat
    37081 attttatctg ccacttcttt atcagtcatt atagtggctg gattactatt tattaataca
    37141 acttcaatcc cttcctcttt caaagactgg caagcttgtg ttcctgaata gtcaaactct
    37201 gcagcttgac ctattattat tggtcctgac cctaatacta aagttttctt tatactatct
    37261 aatttaggca taaatttttc tccttatcta aatggttttt actatctgta aataatacta
    37321 aatctatatt aatgctaaga atttattaaa tatacaatct aaatcttctt ttgttgtgta
    37381 ttctggaatg tactgaacac tatatattgg taattcctta tgtctcattc cttctatagt
    37441 attgtcattt aaatttatgt gagttacttc tgtattttct ggcatttctg aaacattata
    37501 tccatgattt tgagaagtta taaatacctt tccagtttct aaacatttaa ctgggtgatt
    37561 acctcctcta tgtccaaata caagctttga agtctttcct cctaatgcca atgctaaaat
    37621 ttgatgtcct aaacctattc ctgtcaatgg ctttttgcct attaattttt ttatattttc
    37681 aactatatca tttatatcct ctggatttcc tgggccatta gataataaga ttaagtcagg
    37741 atttatattt aaaatttctt ctactgtagt cattgctggt actatagtta tatcacagtt
    37801 acaagcctta agagactcta gtacactatt tttaacacca aagtctataa ctacaacttt
    37861 agctccagta ccttttatat tttcaatttc ttttcttgta acatttttta ctgaatcttt
    37921 gttacaatgc tcttctaatt tagactgaac atcttgtaat gatacatttt ctaaagttat
    37981 aattcctctc ttagttccat tgtttctaat aattttagtt aatgctcttg tatctattcc
    38041 ttcaagtcct attaccttat tttgctttag atatgtgtct atatccattt cacatctaaa
    38101 attatttggg ttatcacttt tttctcttac aataagtcct tttacttgca ccttatttga
    38161 ttcaacatct tctaaattta ttccatagtt tcctataagt ggataagtca tagtaactat
    38221 ttgtccataa taagttgcat ctgtcaatag ttctccatac ccagccattg aagtattaaa
    38281 tacaacttct ccaatagctt cctttagata tccaaatgct tttccttcaa atactgttcc
    38341 atcttctaaa attaatttcc ccttcatgta agctacctcc caaatatttt atctaagcat
    38401 tttctatttc tcttacaata ttaatagctg attctcttgg attttctgct tttgttatag
    38461 gtcttccaac aactaaataa tgagcacctt tttttattgc atctgatgga gttacaactc
    38521 ttttttggtc tccaacctct gctgatttag gtcttattcc tgggcaaact gttataaaat
    38581 cagttccaca tgcttctact attttatcta cttcttgcgc tgaacaaaca accccatcta
    38641 ttcctgattc ttttgccagt ttagctctct ttatagctaa ctcttctgtt gtcatattac
    38701 atcctatgtc ctgtaaatct tggttgctta atgaagttaa aactgttact ccaactacta
    38761 atggcttttc tatatttaat ctttctgctt cttccctagc aattagtgca cattgtctca
    38821 ttccttccat atctgataca tgtattgtca ttaaccaaac attatctctc actgcagctt
    38881 tcactgcact tttcatagta tttggtatat catggaattt aaggtctaaa aatatttttt
    38941 tacctttttc tttaagataa tctattgttt taccttttgt ggcaacgtat tgctctagcc
    39001 ctaccttaaa tatgtctaca ctatcttcta atctgtctat tagttcttta gctttatcaa
    39061 attcgtcagt atcaattgca actatcaatt tttctttacc atttatcatt tttacatctc
    39121 ctttttaata aagcctaaat aatcataaaa aaatcctcac accaaaagag gcataaggat
    39181 tttatctata cataactcta ttcattagct atgagatatc tttcccagtc tctctggact
    39241 agtttaaaag actttattat atttaaattt ttattttata ctcatcaaaa acctctagaa
    39301 gatttactag aggttatata ctaacattat ctataaccct ttagtctctc tggactactt
    39361 taaggaattt agctgtaata tttttcttta tttatataac tttatgtatc tataggataa
    39421 tttaaagttt tatctttttt ataattaaac cactaattgc tttttatgtc aataagtctt
    39481 tgaattattc ttcatcttta tcaaacaatt ttttctcaaa atatgattct gcattatata
    39541 ttgcagcaac tggattatgc ccagttactc tatctttgac aactaaagtt gttataggag
    39601 cttccatata tttcatggct aaagtatcgt gaccaacaca taatccaaga attacattaa
    39661 ggtcaacctt agcttcattt aaaaataaag cttgtcctat tggattgcac attacctcat
    39721 ctgaacactt tgatattgtt tgagatgctt ctagtccaat tgtactctta ggcattgccc
    39781 catttttaca tataacagaa aatacttcaa acccatgatg ttcaaatatt ttttgcactg
    39841 tttgcatttc tctttttagt ccaatacaaa atgctatgcc aatcttttta taaccacact
    39901 ttttagcaaa ctctataatc tcttttagtc tagtatattg acaatacccc tctgcttcaa
    39961 ctaatccagc attataagct atttttctat tctcttcttc ttcataaagt tgctttgctt
    40021 tttgttgtag ttcaatttct ctagttggac atatcttcat taatttatcc atatttccat
    40081 tagaacagcc tctaagatta cattttccac acttatacat atccaatatc cccttccaaa
    40141 attttattca tcttgcgatt cttctttttc tagagttaca ttttcttctt gggtttcttc
    40201 ttctggtttc acatgctcga ctacagttat aaaatatgat tttcctattt tattccttat
    40261 aggaagtttt tcatatgaaa catctatttt attttttata caagctatat caaaatgtct
    40321 taaggctaca cctaagctaa ccttattcat atcttcacag tgtttcttag gtctcttttc
    40381 aaataaatga agacctcgtt catccttatg gaaataccac ggttgagaat tttttacaga
    40441 tggagctctt cttgtcaatt ctaagatatc tttccacttt ctattcctat ttatcttatt
    40501 tatcatatct tttactggct ttctatcagg tctagcatat ctcgtcctaa acaactcttc
    40561 attttttgct ggatatccaa atgctattat tatataagga ttttctattt tgttttcatc
    40621 atcttcttct gtttttattc cagctagata atctaaatca tcttcctcat cttcttcaaa
    40681 attcttattc ccaatatcta caaattccaa gatatcttct ctctttaagt ttccttccaa
    40741 ccagcaagta gctattccca atgttgtaag gcgcaaaact acaccttctg tagcaaatcc
    40801 tatattctgt aaatagtcct cacctttgtc tgatgtaact actatataat gaggtgcctt
    40861 tactttacat tccttaccca tcaaaaaatg tattatatga cctctatcaa caacatgtgc
    40921 tttaatattt aaatcttcat ttaagtattc taaattttta caacttcttt ttatttcttc
    40981 catcaatggc ttttttatct tcttactgga aaaatttctt acagattttc tataaaaaat
    41041 ggcttcataa agctccattt atgccctcct aactgaaatc ttctgacgta taaactctat
    41101 aaatttatat aaatacttta tattttttat agttttattt cctttttcct cctgccctat
    41161 tttgaaattc ataagaatat ctactctagt ttaatatagc attcaaaagg cttaaattct
    41221 accttatcaa tgtctataca ttagcataat ttttttattt gattttctat gctaatttaa
    41281 ataacaacac tagctattac ctttttaatt aagctttgcc agaaaaattt tttttcattt
    41341 ataaatatct atctcattat tatattataa gtttttttcg gtattcttgt atagcttttc
    41401 actaaaaaag tatttttatt tctactttaa tattattatc aacaaaaaac atactgctta
    41461 aataagtttg ttatcctcat aatttgtttt aattaaaata ttttttttat ttctcaatct
    41521 ataaagtata aatattttga atatgaccca aaatctaagt aaagttataa tattttataa
    41581 gttgttcaat attaaaaaca taagtaatgt tataatactt tataagttat tcaatattaa
    41641 aaatatagga ggataaatat gaatttaaat agaaatttag aaaaatacgc tgaattagca
    41701 ataaaagttg gagttaacat acagccaggt caaattctac ttataaagtc accaatagaa
    41761 tgtgctgatt ttgcaagaaa tgcacttaaa gaagcatata aatgtggtgc taaaaatgtt
    41821 tatatagagt ggtctgatga agaaagtact cttattaaat acttatatgc tccagatgaa
    41881 gcatttcatg agttccccaa atggacagca gaacaatatg tagatatagc aaaagaaggt
    41941 ggggcatttt tatcagtcta tgcccaaaat ccagatttat taaaagatgt tgaccctgaa
    42001 agagttgcaa attttcagaa ggcatctgga aaagctttaa aagaatggcg ttcttacact
    42061 ctaactgata aatgtaaatg gaatatagtc tctgtgccaa caaaagattg ggcaaaaaaa
    42121 gtgtttccaa atttatcaga agataaagca atagaaaaac tgtgggatgc tatatttaaa
    42181 tgttcaagag tagatggtca ggaccctata aaagcatggg aagaacataa tgaaaatctt
    42241 aaatctaaaa tggactttct aaataaaaat aactttaaaa ctttaaaata taaatcctca
    42301 aaaactgatt tgactctaga cttgccaaag ggtcatgttt ggctaagtgg agcatccaaa
    42361 gaccctaatg gaataagttt taatccgaat attccaactg aagagatttt tggaatgcca
    42421 cataagttta aagtaaatgg aactgtttat agtacaaaac ctcttgttta tggtggaaac
    42481 ataattgaca atttcttctt aacatttaaa gatggtaaaa taatagattt ctctgctgaa
    42541 aagggactag atacactaga aaaacttatt gaaacggatg aaggctctca ttatttagga
    42601 gaagtcgctc ttgttccata caactcacca atatccaata cggatataat tttttataat
    42661 actctatatg atgaaaatgc ttcttgccac tttgcaattg gttctgctta taagacttgt
    42721 ctggaaggtg gaaataattt aaaagatgaa gaattggatg aacatggtgt aaatgacagt
    42781 ttaacacatg tagattttat gattggttct gctgatatgg atattattgg tgagacttat
    42841 gatggaaagc aaatacagat ttttaaaaat ggtaattggg ctttttaaca tatgatttta
    42901 actaaaaagg gctgatggaa gttataccag acttcccagc cctttttaat gttgatgata
    42961 aatatttttt atattaatta atctcattat tatagattaa ttcctattgt tttaaacata
    43021 agtatctaaa acttatattt atattatata taaaatatcc tatttagtaa aacctttatt
    43081 ttaatttata tgctaaaaat ttaactttgc tatttcaaca gcagactttg catcctttga
    43141 ataataatca gcacccattt cttcagcgta ttcttctgtc aaaactgcac ctcctacaaa
    43201 tacctttgca tttatctcat tatctctcag tgcctgaatt gtatctttca tactttgaac
    43261 agtcgtagtc ataagtgcac ttaatcctac taattttata tttctctttt tagcaacttc
    43321 cactacttct tcaataggga catctttacc taaatctata acttcatatc cataattttc
    43381 tagcattatt tttactatat ttttcccaat atcatggata tcacctttta ctgtagcaac
    43441 tattattttt cctttggata cattattgct acttttactt aataaagttt cctttattgt
    43501 atttaatgaa acttttaccg tttctgctga ttgaatcaat tgtggtaaaa atatatctcc
    43561 actatcatat ctttttccta ctttatcaag agcaggaatt aaaatctcat ctaatatata
    43621 attttcatcc ttctctttta aaagattcag tgttacattt tttgcttcat cttttaatcc
    43681 tctttcaact aaaatatcca agggcaaatc tttatcaatc tttgtagatg aatccgattt
    43741 ttttgaatta tttatattgc tatattgatt tatatagttt atacatcctt tatctgtgtt
    43801 atttaatatt ttaaaagaat tgatagcttc catcatacta tattcatttg gatttataat
    43861 tgctaaatca agccctgccc ctaaagctaa tgttagaaaa gaagcattaa gtgctttcct
    43921 atttggaaga ccaaatgaaa tatttgaaac acctaatata gtttttactc ctagtgtctt
    43981 taccattgtt atagctttta tagtttctat tgcttcttct tgttgtgctg aaacagttaa
    44041 tgataaacag tctataaata tatcttttgg ttttatccca taagaaactg ctctattaac
    44101 tatccttttt gctatttcaa atctttcttc agccttttta ggtatcccct tttcatcaag
    44161 tgtaagtcca accacacatg aaccatactt tttaactatt ggtaatatac tttctaaaga
    44221 ctcctctttg ccatttacag agtttactat tgttctacca ttataatatc taagtccttg
    44281 ctctaaggcc tctacattgg aagaatcaac ttgtaaaggt gtatctacta ctgcttgaat
    44341 ctctcttatt aattttggca ttagtttttt ttcatcaatc tctggaagac caacatttac
    44401 tccaagtatt tgtgctccag catttacttg ctctaaacct aaatttatag cataatcaac
    44461 attttcattt tttaatgctt cttgtagaga ttttctacct gtgggattca atctttctcc
    44521 aacaactgta ggcgattgta cttccacaaa ttttgatggt gaacagacta cacatttatc
    44581 aattttctcg atacatccct ttgtaacaga atttatattc tcagatattt cttttataaa
    44641 acttggattc gtaccacaac atccacctat tatagtagca ccaacttcaa cgaacttttt
    44701 aaccccttca aaaaattctt ttgcatcgac atcatatata gcctctccat caactatatt
    44761 tggaagacct gcatttgctt gaaccataac agggactgta gctcttgatg ctattttttc
    44821 aactattggt aaaagttgtt taggtcctaa tgaacaatta actcctattg catctacacc
    44881 tagaccttct atagtagcca ccatacattc tggcatacat ccagtaaaac ttcttccacc
    44941 ttcatcaaat gtcatagtac agaaaatagg taaatcagag ttttcttttg ctgctaatac
    45001 agctacttta gcttcatata aatccatcat agtctctatt actattacat caactccaga
    45061 tttaacgcct tgtagtactt gtcttttaaa tatttcatat gctttatcaa aacttaaagt
    45121 acccataggc tctagcattt ctcctatagg accaatatct aaagctacat atcttggttt
    45181 agtattatct acattttcta tagcttttct agccaccaaa acagcattgt caatcacttc
    45241 ttctacagta tacttactat ctaattttag ttcattacat ccaaaagtat ttgtaagaat
    45301 tacattcgaa cctgcctcta agtatgctgt atgtatttct ataagtttat ctggattttg
    45361 aagaccaaat acttctggat tttcacctaa ctttaaccct ttttgttgaa gcattgttcc
    45421 cattgcacca tcaaatatta atatattatt tttcagatat tgtcttattt ccacaagagt
    45481 tcgctccctt tctatatgta cagtttctat agtttttaca attttcacac gacttttctt
    45541 tcttagattt atttattctt gtattagaaa tacctataat agccactact gactttctcg
    45601 gtatcataat tccattagaa gtaattgtta gacctatttg tttttgagca ttcaaaacgt
    45661 ttaatatatc tatatttttt tctattggta aatctccata tcctgggcta tatcgcatag
    45721 ttataaattt atcttgcttt aaaaaaacct tttctaaatt attttgaaca atgtcacaca
    45781 cttcttctat tgctgttgtt ccacatgaat caattattag tccttttgtt aaatttgaat
    45841 aagagtgtcg tcttatttct ttttctatat ctatccctat agtagctgcc ataattacac
    45901 actcattaca ttcttttaaa agttcatata catatttact ctctaatgtt aaatttgttc
    45961 cctctaattc tacaacatta tctttattta ttctaatagg ataaatccta gataaatatc
    46021 taggatttat tttattttta gttatagcta cacattcatc aatagtatat ttaaggtctt
    46081 tatctatctc ttgacctctg tattgtaaat atcttaaaac ttcactttga tttatgctta
    46141 tatcttctct tccaattaaa ttattctcca tatatattta aagaaaatcc tttatctaaa
    46201 acatcttttg catattcctt tgccccaaat aaagataagt ctctgtagtt tccacattct
    46261 atagctgtag ctgctggcat tttatctgat tcaagaactt tttttaatgc ttcttctaaa
    46321 ccaatcttaa ctgttttggc atctacatct ccccatagta ttaaataaaa tcctgttcta
    46381 catcccattg gagataagtc tattacacca tctaatgtat ctcttaaata agtagctaaa
    46441 agatgctcta aaccgtgcat agcagcagtt ccaaaacttt ctacatttgg ctgtaaaaat
    46501 ctcaagtcaa atttagttac tttatctccc tttactccat caagtacact acactttctt
    46561 acaaaaggag cttttacttt tgtatggtct agcttaaaac tttctacttt ttccattatt
    46621 taatcctcca ttatacttta aaatttgtta cattaattct aaaaaaaact cttcctatct
    46681 attattgata agaagagtat attatttcaa gtcatctcat cgttgttagc aggaccacct
    46741 gataaaaggt tggtatagga tcactgagct aactctctcc cctaactctt gataagtatc
    46801 ttattaaact gacttaaatt atatattact tttttatttt tgtaaacact ttattgttat
    46861 aatatttata tcacataatc gtacttttat tttataacta agctgattgt attattggca
    46921 gttgtacttc aaacacagtt tgatatttat cgcttgaaac agtaatttct ccatcatgta
    46981 aatttacaat attttttgta atcgcaagac ccaatccaga acctccaaca tttctatttc
    47041 tcgatttttc aactctataa aatctatcaa atatatttgg taaatctaac actggaattg
    47101 gttccccata atttatcact tttacaaccg ccatatcttc ctttttctct gtgactatat
    47161 ctacataatg cccatctttt ccatatttca cagcattagt tataagattt tcaaatgctc
    47221 ttaccaattt atttggatca gcttttacaa ttaatttttc ttcattaaaa ttaattctac
    47281 taaccataaa ttcttgttta aaataaattt caaattgaga tactacttga cttaataact
    47341 ctactaaatt tatttctatt ttgtttaatt ttattgtatt attttgcatt ttagtcaatt
    47401 caaacaaatc atttataaga acatttaaat ttttcgattt ttcatatgca atatcaacat
    47461 agtacatcat ctgaacttca tctttatact taccttcttc tatcactcct aaataaccca
    47521 ttatggatgt caaaggtgtt cttaagtcat gtgacacatt tgttattaaa tctgtttttg
    47581 tctgctgagc ttgtctctct tcaatagtta tatttctaag ttgatggact atattattga
    47641 ttttatgtgc tagttcatca atatcatcct tttctttatc ctttaatata tctaaatcat
    47701 actcaatatc tatacttttt tctaagttac cttcatccat taaatttaca gcacttttaa
    47761 tgatttctaa tttttgattt tttctattag tctttcttat aaaatctaca atttgcttat
    47821 ttctcttgta cgatataatg gaataaattg ctaatgatgt gattgtaaat aaaaatggat
    47881 aaaataaatc ataccctata agacttctga aatctcttgc ttgatataca agttgattaa
    47941 ctacagacat atcactattg acatataatg attttattat atacatactt gttaaaataa
    48001 gcattgaagt taaagcacag cttattataa gatctaatat gggtcgaaac atatgattta
    48061 ataaactttt atttttcaat tttatatcct actccccata cagtatgaat tattttttct
    48121 ccatctgtat atttttcaat tttatccctt attctgctca tatgaaccat tacagtatta
    48181 ttagattggt aatatttctc tttccatacc ttttcaaata tttcttctgt actatatact
    48241 ctgcctttat tatttgctag taaatataat atatcaaatt ctcttgatgt caaaattatt
    48301 tcatttccag ccactgttac actatgtttt tccttatcta taattactac tccaaccttt
    48361 atgacatctt tatcttcatt attagcacca ctaaaataat atgctcttct aatcagcgac
    48421 ttcactctca cagtaagttc aagtgggtta aatggttttg tcaaatagtc atctgcacca
    48481 gtcattatcc cttgtatttt atccatgtct tctgattttg cacttaacat aagtattggt
    48541 atattcaaac tttctctaac ttttctacaa acttctatcc catccatttt aggcatcatt
    48601 atatccaata tcattaaatg aatattttcc ttttttaaaa tatctagagc ctcttttcca
    48661 tcttcagctt taattacatt atatccacta tttaaaagat atatctctat aaggtctctt
    48721 atttcctttt catcatccac aacaagaact gtattattca tttttacaca tcctaactta
    48781 atctttgtat atagctttat tcatataaaa ttatactata ttttagttag tgtaatctat
    48841 aaatttatta atttctaaat ttccaatata atacaaatta tttacacatt tatattattt
    48901 attttgtaaa tattcttcta aagttaatcc ttttgaataa atttcctcag cagcatcctt
    48961 accaacatat ctaatatgcc atggttcata tgctgtacct gttatatctt ctttaccttc
    49021 aggatatctt attataaatc caaacttgtg agcattttct gccaaccatt tagcttcttt
    49081 tgtacttcca acaaaccatc tttctggatt tgttaaatca atacaaagtc cagtttggtg
    49141 ctcactgtaa ccaggctttg ctacatatgc atctgccttt tctcgcccct gagattttac
    49201 ccttcttgta taagtatcca gttgagtatc ataagatctg tatgctgaag accctaaaaa
    49261 ctgtattcct tctgaattag cttgcttaac taaatcttca actgcctttg caggttctcc
    49321 tgccatttgt ttttcttcct ctgttgcttc ttcaacaaaa ggtatgttta ccttagtaat
    49381 attttcagga gtatagtttt tgctaattcc atttgtcttg tttaccaata atatatttga
    49441 tatactattt gagtgatttt tattttgaga atttgcctca acatcttgtt ctgaatcatg
    49501 ttgagcagct actattggat ctttatcttt cttaagtaat ctatctttag atataaatac
    49561 acttataaat actactaaaa taatggctcc tattaattta aattttttca taattaaatc
    49621 ttctcctttt tgttatgttt gctataatgt taattttaga gtataagact taagtatttt
    49681 ataagtaaat cttaaataaa tcttaaattt aatattttta aaggatggtg ataaaatgtc
    49741 tatacaaaaa cttctaagta aagctcgtaa ggctatacaa gattttgata tgatacaaga
    49801 aaatgataaa attgcggttg gactttctgg tggaaaagat agtttaactc tacttcatat
    49861 acttaaaagt tatcaaagat tttctccaca aaactttgaa ttgatagcca taacattaaa
    49921 tactggaggt gttgataatt ctccactaga taaattgtgt aaagaaataa atgttccttt
    49981 ttatgaattt caaactgaca taaaagaaat tgtatttgat ataagacaag aaaagaatcc
    50041 ttgtgcattg tgtgctaatt taagaagagg tgctttgaac gataatgcta aaaaacttgg
    50101 atgtaataaa gtagctcttg gtcatcataa agatgatgca attgagacat ttttaatgtc
    50161 tatgttttat gagggtagag taaattgttt ttccccaaag acatacctag acagacagga
    50221 tttaacagtt ataagaccta tggtttacat agaagagtat atgactaaga aaatttcaaa
    50281 agattctaac tatcctatta tcacaaatcc ctgccctgca aatggtcata ctaggagaga
    50341 gtacataaaa aatcttattg ctaatttaaa taaagagatg cctgatttta aaagaaatgt
    50401 atttggtgct ttaaataatt ctgagaaatt gtttatatgg gataaagaga agataaaaaa
    50461 ttttaaataa taattaaaat ttttaaataa aaaagcaaga ttgtattaat tgtaaataat
    50521 ctttatataa tctacacaat cttacttttt tatttattat atgttattat ctttaatcta
    50581 tattttaata gttcttaata gtatttattt tgtctaatac attaattata acttaaatat
    50641 tttatatatt aatttaatct attaattcat tcaatctact ggtttaatcc atttttacag
    50701 tatatacatc tacatattat aaaaagcaaa attatatttt ctaaatattt tatgttagat
    50761 tataatatta aattctatta tctctggtat attaaaaaat ctaaatggta actttgtact
    50821 tccaagaccc gaactcacat ataaatgatt tccatttata tcataaaacc ctttagtata
    50881 tttccttcca tatggaggag taactaatgc tcccttaata ggtaatctta tttgaccacc
    50941 atgactatgc ccagatagaa ctaaattcat tgtatcttta ctaagcatat ctactaaatc
    51001 tggttcatgt gctaacaata aattaaaatt cttattatct atttgactct ccagatattt
    51061 tatattagga tttccattta atatttcatc aacacctaat atattaatat atttatcatc
    51121 ttttaatttt atttttttat tttcatttac taaaagattg aagttggaat ttttcataat
    51181 ctgcctataa tacctaggaa gtttatacat ataatcatga ttgccaaata ctgaaaattt
    51241 tcccaaccta gcattcatac ttgataaaat agttgtaatt tcatctacac ttggattttt
    51301 attggagtaa tcaattaaat caccagtaaa tactattata tctggattca aagtgttaat
    51361 cttattagct acttttttga gattcttagt tgaataaaaa ctacctattt gagtatctga
    51421 aatttgaact acttttatcc tttcagattt actctcaatt gtatcacttt ttatcaatgt
    51481 tccattttca cttatattat attttttcac aattaataga ctcggctcta tcgcaactga
    51541 gtatataaaa atcaaaataa atattaagat aaaaaatttt aaaaaatact taatttttga
    51601 gttcaaaata tctacctact tatttatttt tttaaactat ttgcattaca gactcaactt
    51661 tgttcatttt tctgtcatgc atttttggct tatttgctga aaaccattta tatgttgctg
    51721 taagaccttc ttttagtaac acatttggta tataaagccc atgttcaata agcttattta
    51781 tatcaagatt aaagtctata tttctaaatg ggaaatacgt tctttctcta aactctactt
    51841 tttccatatc tacatatttt attataggtt ctttgccaat aatttctcca catgtatata
    51901 taaggtcatc ccaagaaatt aactgtggat ttgtcacatt ataggcctct cttacatgag
    51961 gatttttcat tatgctttcc aataccttta ccaaatcccc aatatatata aattgattac
    52021 taacttgttt tccttttggt actggtatct tttcattttt ttctattttt tcaaagaaat
    52081 aatactctct atataaatta ttattttcac catatatata agttggtcta ataattatat
    52141 atggtatagg gctatttata ataaaatctt cagcctcttt tttatttaac ccatattttc
    52201 cccagttttc attttcacct ttctcatttt cttcttttat atttcttcca ctatctttat
    52261 atactgctcc tgctgacaat actatatact tttttaagtt atccatactt ataaaatcaa
    52321 tcagattact tacatcttct tttgtatatg ctgtcatatc atatatgtaa tcatattttc
    52381 ttcctgtgat aatgttttcc atatcttttc gaacttttct atcacaaatt agatgttctt
    52441 taaaaccatt ataatctatt tcctttttac catttgtaag tatatcaatt tgatacccac
    52501 atttaatcaa gcgcttagca agtgcagaac ctatgaagtc gcttccaccc ataattaaaa
    52561 tagatttcat tttaagtgca tcctcctctt ttttaaatga cttccttaac tataatatca
    52621 tatttttaaa tatgcgacac cattttgtaa tatttttgtc tataaaagat atttataaca
    52681 aaatatcttt tatagactct acattttcga caatataagt aggttcactt tcactaagtt
    52741 cttcaaaaga accataccca tacaacactc caatagaatc tatacctatt tttttggctc
    52801 caattatgtc atactttcta tctccaacca taataacttt atctttatct tttacattac
    52861 ataggtctag tacatattgt ataacttcat ttttattcac tcttgtacca tcaagattgc
    52921 tacctgctat atatttaaaa tatctatcta tatcaaaata tctaagtata gtttctgcaa
    52981 aaactgttgg cttagaagtt gctactaata atattttacc atttttatag agcatttcta
    53041 atatctcttt tatattctca tagattttat tttcaaatat ccctttatca gcaaaatatt
    53101 ctctatactt ttcaacagct tctttagttt ttttatcttc aaatttataa tactccttaa
    53161 aagtatcatg taatggtggt cctataaatt ggtctaagtt ttccaaatct tcttttatgc
    53221 caaagctatt taaagaatac tgaatagact ttgttattcc ttctttaggg tctgtaagtg
    53281 ttccatctaa atcgaataat actatttcat aatttttttt catatttcag tcctccatat
    53341 ttgtaaaaag taatatctta tatattaagt ttatctattt taaaaagcta tagtaataga
    53401 aatttctaat actatagctt aagtacccaa ttaaatctta tatatttatt ttaaaaataa
    53461 atttaaacag ctctatttaa ctgttatagc tgaaactgga catccttctt cggcttcttt
    53521 acataaatct tgtaagtctg ctggtatttc tccttcaatt gctactgaat gcccttcatc
    53581 gctcatacta aaaacgtctg gacatgttcc tatacatgct ccacaagcta cacatacatc
    53641 cttatcaaca aatgctttca tactaaaata cctcccctaa aattattatt ttttaattgt
    53701 tgtttcctta gatatttatc caatatatac caatttaaac atatatttat tattttatca
    53761 ctattgttta gtttattata gtattttcgt atccatgtct ataaacttca ctgcttttca
    53821 tccatgattt tacagtatat tgcaatctta attttttatt taaaacaagt tcattattat
    53881 tgtagtcttt tataatatca ttacactcct ccattgtgaa tattgtaaaa tcaccttctc
    53941 caccaatctt tattctgcct ttatttccat gtaaatcgaa aatctttgtt gggtattcag
    54001 ttactttttc tatacacttt tctaaggata tcccaagttc cataatttta tttataacac
    54061 ttaaaagaga atagtttacg tctttcatgt tttttatatg catatcagta cttattaggt
    54121 ctggctctaa accttgcttt attgcttttt taaaaacttt aaaatcaaat tggttcaatc
    54181 catgacatac atcaaacaat actcctctgt tctttgcatt tattactgat tttcttaatg
    54241 tgccagattc tttaaggatt ccatttgtac tatttccata tactggtgta accacatctc
    54301 cttttcccaa aatattcatt acttcatcta tatatgatgg atactctcct atatatacca
    54361 ctaagggtaa atttaatttt ttggcaactt gctttgattt cttaattata tctataccac
    54421 tttcattaga ttgaaattta ctcgctttta cttttatacc ctttatcttt tctatatttt
    54481 tttctacaac tgactcaacc ttgtcaaaat ttataacatc taaagaagtt gattcattca
    54541 atatatttag tccctcattt gtaacattta aaaaagaata tacttctgtt ttacactcat
    54601 ccatatactt ttttttaaaa tcatagtagt ttaaaggacc agctgtacct gcatcaaata
    54661 taactgtaga acctttttca attccaattg aatcagaatc catacctgta ggtaattttc
    54721 tcttataaca atgggtgtga acatctataa atcctgggga aatatacata ttatcggcat
    54781 ttattatctc gaactcttta tttgtaagta atttttcttt tcttattttc tctttaaggt
    54841 tcaatccaat agcatatatt ttcccatgta aaaccaagat atctcttttt tcaaatttga
    54901 taccatctgt tggagatatt attgtcccat ttcttataag caaatttttc atttattcca
    54961 catcctaatt acaatataat ttctctaata gcttttctta cataaagaaa ataagcctaa
    55021 aaataaaaat attttagact tattcactat attatactat attaattata ttaaattaaa
    55081 taaaatcttt aatttacttt aagaagcaat taagtatctg ttgttcggct tcttcttcag
    55141 taagacctaa tgtcatcatc tttagtattt gatctcctgc aatctttcca atagctgctt
    55201 catgtactaa aagagcatct ggatgatttg cagcaatttc aggtatagca ctaatttgag
    55261 aatttcccat aattattgaa tcacattgaa catgtgcaga acaaagggaa tccccaataa
    55321 cacaaggatt aaacttttgc ttagaattat cttgcccaac tgtacgagaa actacttgag
    55381 cagaagagtt ttccccctct aagataacct tcatattaga ttctgcaact tgattaccat
    55441 gagttaataa tttctcaaat acaactattt tagctccttt acctaggttt gcatccattt
    55501 ctcttttagt tgaatctaca cccttaattt gaattgtttc catttcacaa tatgaatcct
    55561 catccatatt gacaatagta actggattta aaattctctt tccttctcca tcaccttcac
    55621 cataatgttt ttctacatat tttaggcgag ctcctttacg taagaaaaat ctatgtatac
    55681 cattatgttc actatcatca caaccacagt tatgtatacc acacccagca attataacaa
    55741 catctgcatt ttctccaatt tcaaaatcat tatataccat atcctgtaca ccagtctctg
    55801 ttactattac tggtatatgt acattttctc cttttgtatt tggctttact ataatatcta
    55861 ttccaggttt atcagtttta ggaattatag ttacattttc agttgaatgt ctctctactc
    55921 cttgcccatt tttacgtata ttgtatgctc ctaatggagt tccacgaagt tcagcaactt
    55981 tttctaaaag atttaaatct attttgttca tttaatttgc cttcctttct aattaattca
    56041 tgtctttcat taattcacaa cttgaattaa cttgacacat gatttcaggt aatatagcct
    56101 cttttgttcc atggcttttt actgaaccat cttgtagaac aattatttca tcagctaact
    56161 ctaatattct ttcttggtga gaaattatga ttatagtctg gttagactct tcatgtattt
    56221 tcttaaaact ttcattgagt ttcccaaagc tccaaaggtc tatccctgct tcaggttcat
    56281 caaatataga aacctttaaa tctctagcaa aaagagttgc tatctcaatt cttttcattt
    56341 cccctccaga aagagagtta tcaacttcac gatttaaata gtcttttgaa cacaatccaa
    56401 catctgtaag atattggcaa catacatcag tagacaatgg ttttccatga gcaagtgtaa
    56461 gtaaattttc aactgtcata ccttttattc ttggtggttg ttgaaatgca tatcctattc
    56521 ctttttttgc tctttctgta atactaagat ttgttatatc ttctccatct aaaattatct
    56581 gaccactcgt agctttttca attcccataa taagcttagc aattgtagat tttcctccac
    56641 catttggccc tgtgataaca ataagttttc ccctctcaaa tgaaaggcta acatcattta
    56701 ttattcctag ttcctcattg ttagactcaa cattaaagga aagatttttt atttcaagca
    56761 taatacccct cttttctata aaatattaca ttaatatcca ttcttttatt atatcagtaa
    56821 tttttatata tcagcaactc taagcagata aaatgtatat taaataataa aacaaagtta
    56881 ttgaaaagta aaaaagatga gttttatgta aactcatctc agaaatttag tatatcaact
    56941 tatatgtatt taaaaattag ccttctatat actctaatat tattttttga ttatctttta
    57001 acataatttc ttctttttta gattctattc cattcttttt tatcttcacc tcagctacaa
    57061 aggcatcatt accttcaacc atttctaata gttcataagc ttttttacga cctattttag
    57121 ttgctatatc ttttagtact gccattttct taaacatttt atcacctcta ctcaaaattt
    57181 acacttaatt tttaattata taattctaat cctagactag tttataataa ttattgtctc
    57241 taattatatc agatattgag taaatttaat actttgtaaa attatttatc ctctctgacc
    57301 aaataatcat ttccatcttg gtctttaaaa gaaaacatcc ttccatatgg catctccatt
    57361 aattccccaa cctcaacctc gttatttgcc atttgttcat aagttccatt aatatctctt
    57421 gtacttaaaa ttattgatgg atgagataca tttatctctt tgttttgtct catcattaga
    57481 tttttatcat acaacacaaa tgttgtaaat tcatttttac ttggtgctac ttccacccat
    57541 ttcatatttg gacccatttc cttttcataa gttactacaa agttcaattt ttctgtccaa
    57601 aacaactttg catcctcttg attatttaca tagagagtta tttttcctat tttatttatc
    57661 ataacaatac ccacctttat tattaaataa atacctaata ttttttatat ttaataatat
    57721 acccatatat attattttct atagatatat atttctaata tttatcgcca aattatgtaa
    57781 ttctgctcaa tatataattt atagtatcca tactattata ggctttattt atgtaattta
    57841 atcaccttat atatcatact tataattatg ataataacta cataaaaaat cacttctaaa
    57901 tagagttatc catctaaaag tgatttcata tattctatgc aactttgtgt tttagccact
    57961 ttgttcctct taatcttaaa caatataaag tccctctaac aagccaatca gtatacatac
    58021 ctatccaaat tcctaacact ccaaattcta atacaacccc caatacgtaa cctgtaaaaa
    58081 ttctaaaaat ccacatacct attactgcag tcatcattgt atatctagta tcaccggctc
    58141 cctttagccc tgctgataat acaaaggata taggccatat taccatagct aaacaatcac
    58201 ttcgaattaa ttgtgttgag atgtcaatta cactagcatt ttgagtatac atacttgaaa
    58261 tccaaggtgc aattggtata aatatagctc ctactataaa taaacaaatt gttgcaaatt
    58321 tagttaaata taataaagta ctcttagctc ctttaatatc tccacgtcct atatactgtc
    58381 ccaccaaggt tgtagcagac aaagctaaag cattcccaat tacatttatt atggatgcaa
    58441 ttgacattcc tattgcattg gatgcaattg ctgatgttcc catagtaact ataaatactt
    58501 gaacaatcaa tttacctgca ttaaaaagta gttgttcaac tccagctggt attcctatat
    58561 taaatataga cttttgagtt tcaacatcaa tcttaaatgg aaatatttta cccatagata
    58621 taaattcgtt tcctttaaat aaagctctta taattactat acatccaatt attctagcta
    58681 tagcaataga tatagctgca cctgttatgc cataggatgg tgtttgcaaa tttattccta
    58741 gaggatttaa atcaatacca tatataagta tatatcctaa tgttacattt actatattca
    58801 taaacatagt cacatacata ggtttcttta catctccaca ccctcttaaa agaccatttg
    58861 ctatttgttg tgtcgcaata aatggatacg taaataatgt aaactctatg tataattttg
    58921 catttgattt taccaattct tctgcggaac cataaaataa attaattaca ccagttctaa
    58981 aaacccacat caatattgtt attgtaaagg ataatattac tccagacact agagcctgct
    59041 taatagtttc attcaccttt tcaccatctt tttttcctat ttgttgagct acaacaacag
    59101 tagctccaac agatagtgca gcaaatacag atataaatat attgtttatt gaatcaacca
    59161 ttccaactgc agatacagct tcttccccaa tatgacctgc catcattgta ttaaatactc
    59221 ctaaaagcat gacaaacgtc tgctctatca ttataggaat tgctaacttt ataacatttt
    59281 tccttataaa catccctatt cctcttttcc ctctttatat aaatttaatt tttaattagt
    59341 ttatcgaact gtaaattgac aatgcaaagt ttagaattgt attgttcgca ttataatata
    59401 ctgctttcaa ataatataaa tcttgtttaa tatcatttga acacagtata tctttataat
    59461 tatcttatgt ttattatttt ttatggatac cacatttaaa atatttatta atttaatata
    59521 aatattttcc gttattatta aaagtagcta tatttcgtta atattgtatc actatcgtgt
    59581 agcaataaca ataagtacat tttatatatt ttttataata ttatttaatc atccaaactt
    59641 ttaccacttt ttcaatatat aaaatattac actttagatg taacaaaatc gcatccattt
    59701 aaatattgta ttttatagac taattttagg atgtgatact ttggttaatt taaatgcaaa
    59761 taatctaaat gtaaatatgg tttatcctat ttcttccaca gcacccatac gtttcagaag
    59821 atatactgtt tcatatttag aaaacatgga aaatatttat ttaactatag caacatactt
    59881 tgatacactt ggtttaggca caatgaatat cgacaaagtt tatggtcaat gggcttggtt
    59941 tactgataat atatatgatt taaatctatt tgtttttgta ggaaattatc cttatcaaat
    60001 tgctaaaaac agatatgata catttgtaga tattttgcca gtagctatta cttctattgt
    60061 caatggagat agaatatttt taatttcaaa tccttgtttg ttaaattcaa aaattacagt
    60121 tagatttata tctagtcatc ctactcttaa tagaacagtt gattatggca atattagaga
    60181 ttttattttt taaaactgca acatgatgtg ctattaaaaa ataccctaaa tttaatgata
    60241 ttattaaata actagaatat ttttaattgg cacatcatgc ctagctatta taaattcata
    60301 atattatttt acttaagata agtactgtat tttatctact tgtatatttt tatttaccta
    60361 gtttctcagt tctatatcct atttctttat tagctagctt ataaccttct tgtatcattt
    60421 cttctctcgt taatgcactc cctaataaat tgtcaatact cagttgcttt ctatcactaa
    60481 aatacttatc caataatgat tcctctatat cttcctgata tgccaaattt ccttcatgca
    60541 ttatatattt tacatcaaaa ccttctttat ttaatacttt tcccaccaag atacttctat
    60601 gacatcttat aggttcttgc atagctccta ataaaactat cctataaccc atttgacaac
    60661 ccttttttaa tctttctatt ccgtttaaaa aaatatcttc ttttgctact ttctcaaaat
    60721 ctgcataacc ttcttgcgta tatacatctt tattagttct tttagctcca aattcttttc
    60781 ccatgtatat atataaaaat ccggaatttc taagagattc gttaaatgcc tctttattgt
    60841 attgaatatt atatttagaa tatggagttt ctctaatgtc aactacacaa tttatgtcat
    60901 atttttttat catattaata agcttatcat atggataatt tgaatgtcct attgcgaata
    60961 tttccatata aacaccctcc tattctttga aattagtatc tatatatgta gatacccgat
    61021 ttacttaaaa ttaaataaag tattacttta aatacccaat atcttttttt cttgatattg
    61081 tatatttttt tactttatat ccttaaaaaa atatacaact ttatatataa aacatcaata
    61141 tttatatata ttttgtatat taaatacaat tatttataaa gtctttaata aaaatttata
    61201 aatagagcat ctttagaaaa ctattataat aaaaaaataa gaggctgtct aaaaatatag
    61261 ataagccttt tttcataatc gtaattataa aaatatactt gatttcatat gaaaaagagt
    61321 gtctcataaa ctaaaatgtt tactttgaga caccaccttt tttattctta tttatacttt
    61381 gtagacatat tataacatgc gtttcactgg tatctgaact tctgtcaacc agtcttcttc
    61441 actctcttta ttccatatac catctatata agattctcta ggatttccaa ccactttata
    61501 tttattgtcc tctatccatt taaatgcatc tgcgtaagtt tttcctaatg ttgaatatgg
    61561 accattgtgt aatatacaaa ctgcattttc aactgcatct aattctttaa attttaatat
    61621 atccgtatct tcctttaaat catttatagc ttgacacatt tcaacatcca tatcagtctc
    61681 tctatattca ccatcatgat atatattaaa acaataatca ggaactgcac atgtacaccc
    61741 tgttttcatc atttcttcag ccattacatt tggaaataga ttaaaaagtt catcataccc
    61801 ctttaacact tttctcatag aagcaacttt aacttttggt aaagacttaa taattacatc
    61861 attcatattg acttctcctt tcaaattcat aaggtaatta cgtagtttaa ttattttata
    61921 ttcttcactt ttaattacat ccaacatttc tttttcttta ttcattaaaa tattttcaac
    61981 ttcttcagga ttttctacag ctttttttat ctcagctaat gataaaccca tttgttttaa
    62041 agttattatc tgatgtaatt taggtaattg ctctgttgta taatacctat attttgtaaa
    62101 ttcatctaca tattctggtt ttaacaaccc tatatcttca taatacctta atgtctttgt
    62161 agttatttta tttattttag aaaacattcc tatagaaaac atctcatcac ctgcgcatta
    62221 gatttgatta atttaagttt aaactttccc cttaggttaa agtcaataca aaataaatta
    62281 taattgtatt aattcttcaa ttctatctcc actcaatacc aaaatatccc ctgtttttat
    62341 aatggttgaa cctttaggta ttaaaacctg ttcctttctt tttatcataa ctattaatat
    62401 atcgtctggt atattagaat ctattatact cttattagcc catttattac cctcatcaac
    62461 atttacttcg ataagtttag agctaatttc tccatcataa tcattaaatg tttttaatac
    62521 aggtgtatta tcatcaacta aatccaactt tctggctaca gttggtatca atgaaccttg
    62581 tattcctact gaaagcaacg ctataaaaaa tattatatgg aatatgtcat tctctatggc
    62641 tactccttga gtaacagcat aaattgcaaa aactactgaa gccgcacctc taagacctac
    62701 ccacgatatg aataattttt ctttatttga gaatttaaat tttgctagaa ctataaatgt
    62761 tgctaatggt cttgcaataa ctatcatgaa tatagatatg gccattgcta taccaaatac
    62821 atttggtaac ttagaaggga aagataacaa tcctaacata aagaataaca ttatttgcat
    62881 aatccatgaa attccatcaa agaaatgaac caaacttttt ttaagaggta tcctactatt
    62941 tcctataata atacctgaaa tgtaaacact taaatatcca tttcctccaa gatactcgct
    63001 aaatgcataa gaaagtattg caatagctgt tataaatata gtataaaatc cttctatctc
    63061 gaagtttgaa tgtcttaaaa tataaacaga taattttgct agtataaatc ctatagctaa
    63121 cccaaaagct atttgtttta ttaccattgg tgcaataagt tctaccccag aattattcat
    63181 caatgtcaaa agaattagtg tcaacatata cgctattggg tcattacttc cactttcaac
    63241 ttctaataat gatgcaagtg aaccctttaa gtttagtttt tgagaacgca acacagcaaa
    63301 cacagatgca gcatctgttg aagctgcaat tgccccaatt aataaacctt caagtaaact
    63361 agttcctatt accatgtggc aaaacatacc tgtaacccca gccgtaatta ttacacccag
    63421 tgaagacatt aatatagaag gtatagcaac aggtttagcc atattccagt ttgttccaaa
    63481 ccctccaaaa aacattataa atattagtcc aatagaacat aattgttttg tcagttcata
    63541 gttatcaaaa tagattccta ccatcccatc agacccaaat aacatcccta gtaagataaa
    63601 aatcaataat attggtactc caaatttata caataccttg ctagaagtta tacaaatcaa
    63661 tagtactaac ccacttataa tcattaattc tgtcatagca tcaactccat ttcatataaa
    63721 ttttgtagat taatttacat ctacaatttt attacttatt caaatataac atgacataaa
    63781 ttttataagt tatggtacat aaagttcaac aaaaatttca caatacatta aaatgtgagt
    63841 gttatactta aatttatata tttgttttaa ttatattcat ttaatattta taatttgaat
    63901 ttttttttat taatatttca ttttttagtt gattgtttaa tatatttcat tttttatagt
    63961 aattttatat ataactacga aatataaagt aattagataa gtaacaagaa actaacatgt
    64021 aatgttaggt actctagata tgaaatctta aaacttttaa atattgaaat atttctttat
    64081 atatattatt gtatatttct tataaattat tgtcaattta ttttttctta aaaataaaat
    64141 aattgcataa tttatttcaa attatgcaat ttataaaatt tattcccaaa actcttttac
    64201 tgcttcgatt atctcactat aaggcaataa agtcgcttcg attattgatt tatttgtctt
    64261 ttctaaataa tacttaatca tataatatcc acaagcatat cctgcacaat aaggcaaccc
    64321 tactggaaaa tatccttgca ttttagctat atcatcacca taaagataag atgttatatt
    64381 ttgaaatcca gtttctttta aagcactttt tattattggc tttatataag tatttaatgt
    64441 ttcgatatct gttctactga cccaaggtcc taacatttcc tctccaaaca tccatgttgc
    64501 aaaattttct gcaagacctt catttatcat catttcttct aatgttatat tattattcca
    64561 ctctataaac tgaaatctaa tattgtgatt acattcatgt gctattaata ctggtaatct
    64621 attgatagta tattcattag gaaccaatga tagaaatata tatccaggaa tcccaccatc
    64681 accccagtat ccatcactta atattgtata aggactattt ggattcgcca ataatattga
    64741 atatttatag tccttaatat ttaaatcata cccttctttt ataaaagcat ttatagagtt
    64801 ttctatggtt tctttacaag tggcccaaat tttatcagca gataacacat ttatattctt
    64861 tttttgtttt tcatcaatat ccttaggcga taaaacccct aacatttcac ttgctataat
    64921 tacatcatat ccacctttct gtctagcaac tattggaaca ttcatacact tccacttaaa
    64981 ttcaaaaggc ttcatcaatt catatctgta tatatcttct tttttgttta ttggtgcatc
    65041 cattatcttc ttgtatattt tatctgagcg aatttctata atattcatat ttaccatctc
    65101 ctttaagaca aagtatatac aattacgtag cgtcaatgtc aatatacatt ttttataatg
    65161 tcaccttaat ctttttatta tattttatcc tgtaaacctt ccgtacatat aacatttttt
    65221 taaattaata aatttgtata atataataat aaaaaattat atacttcaag gtttccctat
    65281 gatacaattt actaaatcat aatttagatt atcaggtgca actataaatt attctatatc
    65341 aaggaggata tattggtcat gctaactcca tatttaatat ttaatggtac ttgtgaaaaa
    65401 gcatttaatt tttatgctga ggctttcgga ggaggaaaaa ctatatttgc gcgattagac
    65461 agcaatccaa acaatcctat tatgcacgca agtgttactt tcacaaaata cgaaggttgt
    65521 ataatgggtg cggatacaga caagcctgtt gtaatttctg gcatggcgat ttgtgttgtt
    65581 ctaccatctc gagaagcgat agaagaaata tctgtaaaac ttgccgaagg tggtacactt
    65641 gtacaagaat ttttaccaca cccaccacca catcaaaatg atggcgctgc tgaagtactt
    65701 gataggtatg ggtatacttg gtatttaagt acatagttaa aataaaaaaa cgacatctaa
    65761 taatcataat atagaacaga taaatttcaa tttaatagtg tagtaaaaaa gtcgtgtcct
    65821 aagactgtca cgacttttat ttgtataaaa atttaagtaa atatttttgc gaataaatga
    65881 gaagtataac tttaaattaa tttcatatgt agtattggaa atgggttacc ttgtccatct
    65941 atttcagacc tatcaaacac tttaaatcct aagtgaatat aaaatcctac agctttttta
    66001 ttctgctcat ttacatcaac atattttaca ttgtatttat ctatagcata ttttattaat
    66061 tttttaccaa taccatttcc tctatattta tcttctataa agagcatctc aattttatct
    66121 ttatctattc ctataaagcc ctttattata tcattatctt ttatacaaac tatattttct
    66181 acttctttaa gtccaatctc aactaatggt atcaaagatt caatatcttt ttctgttaaa
    66241 aaatcatgcg ttgctcttac tgatgattcc cagacttgca acatttcgtc atattctgtt
    66301 gaatttattt gtttcatttc aagaattatt ccagatttca taaaaatttc ctccctatat
    66361 cacttttaat tgcataacac ttttttatac aaaacttaga aaggactacg aaggttaaat
    66421 atcatagtcc tcattaagtt taaatattat ctattatata ttattatact ataatctagc
    66481 aaattcacta caataattca catcagctta tactaaaggt gcttctattt gagtagtaaa
    66541 aaggaattta tgacaatgac catcattaac agtagtttct ccacatatta agtgaatgtg
    66601 tttatcagtt ccaggtattc taattgctgg ccctgttgta acacatatct tatggaaatg
    66661 gtcaaggaaa tctacattat catttatttt atgaacatga tttgtacctc catttattgg
    66721 tatagcttca ccagtaaccc ctgctgcacg gtggttatgt ctatcctcac attcttctgc
    66781 taacttaaca ctttctgaat attcatgaac atgtgtcatt tcctcataat cattatagca
    66841 cttgttgcac ttattgtatt tataatcatc atacacataa tctctttcac atttataatt
    66901 ttgataactc atttttatat ctctccctat attatattaa gaaattcttg taaagaatct
    66961 ctaatgtata atatgaatta aagtcaaaaa agttcatgtt ttgtaaaatt atgtattaat
    67021 ttaattattt ttcccagtat ttagggcgta ttagtgaagc tggtaacttc gtactagaat
    67081 ctccttttgc agtatttact tgtaattgtg ttaaaaatat actattttca agattagcac
    67141 ctctaatatc acaatctctt aagtctgctc ctattaaatt tgccccactc aaatctgtat
    67201 ttcttaaatt tgctgctatt agaaatctac ctcttaaatc tgctcctatt agatttattt
    67261 ttctaaggtc tgctcctatt aaatctatat ttttacttaa ttttttcatg tttttaagag
    67321 tagaagtctt gataaatgat ttaacctttc ccatcacaga ctcactcgct tgtgataata
    67381 ataaattaac ctttattcta tgtgatatga tatccaaatt aattatctgt tctgggctca
    67441 tattagttat cttttcagtt tcttcaatta ttaaatcaat ttttgatttg attgtatctg
    67501 atatatttaa tacagatgct tctttaagat accataacat ttcatgtaat tgtctcataa
    67561 ttaaaaacac ttcagacatt tgatttgtta gttctgatga ttgcatccag tctatcccct
    67621 tatatgttac ttgtgctact ttttgacctg aaccaaaaca atcatatgca gtacaccctt
    67681 taaaacctcg cttcataaga ctattatgta cagaacattt aaaatctggt tgtaaattaa
    67741 tacagggttt tcctgattcc ttatcgatag gaaatccatc tgaagcagaa aaataaagag
    67801 ctacacagca aagtccaaag cacttcttac agtcaatctt tagctcctca aataacttat
    67861 tatcatatat atttttatgt atatctatca tattttttct cctcttaaac tttattttta
    67921 atattatatt atctataact aaatgataat caagtccctt atttaacagt tcttattctt
    67981 tattataatt taaggtatta tttattccaa tagcatacat aagtttcttt atacttgtaa
    68041 caataccttt atactaagac ttattttaaa tgtagcactg taataataaa ttgtttatat
    68101 actcataatg tatttg
//
LOCUS       DAMJWS010000011        64544 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMJWS010000011 DAMJWS010000000
VERSION     DAMJWS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.011"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            159..1355
                     /locus_tag="PC399_000758"
     CDS             159..1355
                     /locus_tag="PC399_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF2591635.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1448..1972
                     /locus_tag="PC399_000759"
     CDS             1448..1972
                     /locus_tag="PC399_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF2591636.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1997..2173
                     /gene="rpmF"
                     /locus_tag="PC399_000760"
     CDS             1997..2173
                     /gene="rpmF"
                     /locus_tag="PC399_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF2591637.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2380..2937
                     /gene="fapR"
                     /locus_tag="PC399_000761"
     CDS             2380..2937
                     /gene="fapR"
                     /locus_tag="PC399_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF2591638.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2948..3970
                     /gene="plsX"
                     /locus_tag="PC399_000762"
     CDS             2948..3970
                     /gene="plsX"
                     /locus_tag="PC399_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF2591639.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3996..4982
                     /locus_tag="PC399_000763"
     CDS             3996..4982
                     /locus_tag="PC399_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF2591640.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5091..6020
                     /gene="fabK"
                     /locus_tag="PC399_000764"
     CDS             5091..6020
                     /gene="fabK"
                     /locus_tag="PC399_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF2591641.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            6036..6986
                     /gene="fabD"
                     /locus_tag="PC399_000765"
     CDS             6036..6986
                     /gene="fabD"
                     /locus_tag="PC399_000765"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF2591642.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQVDLKYSACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQDTVPTGVGAMAAILGLDRSILENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLREAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6986..7735
                     /gene="fabG"
                     /locus_tag="PC399_000766"
     CDS             6986..7735
                     /gene="fabG"
                     /locus_tag="PC399_000766"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF2591643.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7789..8013
                     /gene="acpP"
                     /locus_tag="PC399_000767"
     CDS             7789..8013
                     /gene="acpP"
                     /locus_tag="PC399_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2591644.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8042..9280
                     /gene="fabF"
                     /locus_tag="PC399_000768"
     CDS             8042..9280
                     /gene="fabF"
                     /locus_tag="PC399_000768"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF2591645.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9506..11224
                     /locus_tag="PC399_000769"
     CDS             9506..11224
                     /locus_tag="PC399_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2591646.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKHDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11255..13255
                     /locus_tag="PC399_000770"
     CDS             11255..13255
                     /locus_tag="PC399_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2591647.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSELSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13606..13851
                     /locus_tag="PC399_000771"
     CDS             13606..13851
                     /locus_tag="PC399_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2591648.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13886..14611
                     /locus_tag="PC399_000772"
     CDS             13886..14611
                     /locus_tag="PC399_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2591649.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14633..15946
                     /locus_tag="PC399_000773"
     CDS             14633..15946
                     /locus_tag="PC399_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2591650.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVVMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYDENLKKIV"
     gene            16152..16691
                     /locus_tag="PC399_000774"
     CDS             16152..16691
                     /locus_tag="PC399_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2591651.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16780..18765
                     /locus_tag="PC399_000775"
     CDS             16780..18765
                     /locus_tag="PC399_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDF2591652.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18989..19957
                     /gene="spoIIIAA"
                     /locus_tag="PC399_000776"
     CDS             18989..19957
                     /gene="spoIIIAA"
                     /locus_tag="PC399_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF2591653.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19947..20468
                     /locus_tag="PC399_000777"
     CDS             19947..20468
                     /locus_tag="PC399_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF2591654.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20485..20682
                     /gene="spoIIIAC"
                     /locus_tag="PC399_000778"
     CDS             20485..20682
                     /gene="spoIIIAC"
                     /locus_tag="PC399_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF2591655.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20698..21084
                     /gene="spoIIIAD"
                     /locus_tag="PC399_000779"
     CDS             20698..21084
                     /gene="spoIIIAD"
                     /locus_tag="PC399_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF2591656.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21169..22374
                     /gene="spoIIIAE"
                     /locus_tag="PC399_000780"
     CDS             21169..22374
                     /gene="spoIIIAE"
                     /locus_tag="PC399_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF2591657.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22434..23051
                     /locus_tag="PC399_000781"
     CDS             22434..23051
                     /locus_tag="PC399_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF2591658.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            23063..23617
                     /locus_tag="PC399_000782"
     CDS             23063..23617
                     /locus_tag="PC399_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF2591659.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23644..24333
                     /locus_tag="PC399_000783"
     CDS             23644..24333
                     /locus_tag="PC399_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF2591660.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSYSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24443..24805
                     /locus_tag="PC399_000784"
     CDS             24443..24805
                     /locus_tag="PC399_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2591661.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24890..25396
                     /gene="nusB"
                     /locus_tag="PC399_000785"
     CDS             24890..25396
                     /gene="nusB"
                     /locus_tag="PC399_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF2591662.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25380..26450
                     /locus_tag="PC399_000786"
     CDS             25380..26450
                     /locus_tag="PC399_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF2591663.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26447..27652
                     /locus_tag="PC399_000787"
     CDS             26447..27652
                     /locus_tag="PC399_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF2591664.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27654..27869
                     /gene="xseB"
                     /locus_tag="PC399_000788"
     CDS             27654..27869
                     /gene="xseB"
                     /locus_tag="PC399_000788"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF2591665.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27872..28759
                     /locus_tag="PC399_000789"
     CDS             27872..28759
                     /locus_tag="PC399_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF2591666.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28789..29226
                     /locus_tag="PC399_000790"
     CDS             28789..29226
                     /locus_tag="PC399_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF2591667.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29260..31125
                     /locus_tag="PC399_000791"
     CDS             29260..31125
                     /locus_tag="PC399_000791"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF2591668.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31258..32070
                     /locus_tag="PC399_000792"
     CDS             31258..32070
                     /locus_tag="PC399_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HDF2591669.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32090..33769
                     /gene="recN"
                     /locus_tag="PC399_000793"
     CDS             32090..33769
                     /gene="recN"
                     /locus_tag="PC399_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF2591670.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33984..34850
                     /locus_tag="PC399_000794"
     CDS             33984..34850
                     /locus_tag="PC399_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591671.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDIDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34943..35434)
                     /locus_tag="PC399_000795"
     CDS             complement(34943..35434)
                     /locus_tag="PC399_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2591672.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35495..35935)
                     /locus_tag="PC399_000796"
     CDS             complement(35495..35935)
                     /locus_tag="PC399_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2591673.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36096..37154
                     /locus_tag="PC399_000797"
     CDS             36096..37154
                     /locus_tag="PC399_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2591674.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGGAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37296..38120
                     /gene="spo0A"
                     /locus_tag="PC399_000798"
     CDS             37296..38120
                     /gene="spo0A"
                     /locus_tag="PC399_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF2591675.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38533..39654
                     /locus_tag="PC399_000799"
     CDS             38533..39654
                     /locus_tag="PC399_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591676.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39672..40547
                     /locus_tag="PC399_000800"
     CDS             39672..40547
                     /locus_tag="PC399_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF2591677.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40571..41263
                     /locus_tag="PC399_000801"
     CDS             40571..41263
                     /locus_tag="PC399_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2591678.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41271..42095
                     /locus_tag="PC399_000802"
     CDS             41271..42095
                     /locus_tag="PC399_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2591679.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42100..43167
                     /locus_tag="PC399_000803"
     CDS             42100..43167
                     /locus_tag="PC399_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF2591680.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43169..43705
                     /locus_tag="PC399_000804"
     CDS             43169..43705
                     /locus_tag="PC399_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2591681.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43773..44378
                     /locus_tag="PC399_000805"
     CDS             43773..44378
                     /locus_tag="PC399_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF2591682.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44390..45304
                     /locus_tag="PC399_000806"
     CDS             44390..45304
                     /locus_tag="PC399_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2591683.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45346..46518
                     /locus_tag="PC399_000807"
     CDS             45346..46518
                     /locus_tag="PC399_000807"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF2591684.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46565..47377
                     /locus_tag="PC399_000808"
     CDS             46565..47377
                     /locus_tag="PC399_000808"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF2591685.1"
                     /translation="MFEKIAQSSKFINSKSDIKPKIGLILGSGLGDLANDIEDPVAIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47581..48906
                     /locus_tag="PC399_000809"
     CDS             47581..48906
                     /locus_tag="PC399_000809"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF2591686.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49119..50198
                     /gene="mltG"
                     /locus_tag="PC399_000810"
     CDS             49119..50198
                     /gene="mltG"
                     /locus_tag="PC399_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF2591687.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50370..51044
                     /locus_tag="PC399_000811"
     CDS             50370..51044
                     /locus_tag="PC399_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF2591688.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            51031..52278
                     /locus_tag="PC399_000812"
     CDS             51031..52278
                     /locus_tag="PC399_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2591689.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52350..54014
                     /locus_tag="PC399_000813"
     CDS             52350..54014
                     /locus_tag="PC399_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF2591690.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54111..54395)
                     /locus_tag="PC399_000814"
     CDS             complement(54111..54395)
                     /locus_tag="PC399_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2591691.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54418..55935)
                     /locus_tag="PC399_000815"
     CDS             complement(54418..55935)
                     /locus_tag="PC399_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2591692.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(56060..56881)
                     /locus_tag="PC399_000816"
     CDS             complement(56060..56881)
                     /locus_tag="PC399_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2591693.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIKIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            57072..58099
                     /locus_tag="PC399_000817"
                     /pseudo
     CDS             57072..58099
                     /locus_tag="PC399_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
     gene            58171..58323
                     /locus_tag="PC399_000818"
     CDS             58171..58323
                     /locus_tag="PC399_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591694.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRTKSDLRVEFKF
                     IFKSKK"
     repeat_region   58977..60125
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=58977..59005
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            complement(60412..60636)
                     /locus_tag="PC399_000819"
     CDS             complement(60412..60636)
                     /locus_tag="PC399_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2591695.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRE
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            61315..61470
                     /locus_tag="PC399_000820"
     CDS             61315..61470
                     /locus_tag="PC399_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDF2591696.1"
                     /translation="MALEVKIEVKIIKDVVILNITHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     gene            61716..61967
                     /locus_tag="PC399_000821"
     CDS             61716..61967
                     /locus_tag="PC399_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591697.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            62609..62818
                     /locus_tag="PC399_000822"
     CDS             62609..62818
                     /locus_tag="PC399_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591698.1"
                     /translation="MMGLKLKRRYKNVLIYINIRTIININCKIKFIELYLTFNELKNK
                     LANNEKTTVINENNKAFSGLVDFSN"
     gene            complement(63187..63570)
                     /locus_tag="PC399_000823"
     CDS             complement(63187..63570)
                     /locus_tag="PC399_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591699.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            63697..64026
                     /locus_tag="PC399_000824"
     CDS             63697..64026
                     /locus_tag="PC399_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591700.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVF"
     gene            64223..64432
                     /locus_tag="PC399_000825"
     CDS             64223..64432
                     /locus_tag="PC399_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591701.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFESIATLVRGGSSWSFNFIV"
BASE COUNT        25792 a         6725 c        11566 g        20461 t
ORIGIN      
        1 ttatattaaa aggtgatata ttatttatga aaatcgttta atgtatacaa taatatatct
       61 ataacaaaat aataaaagat agagttttat ttagacattt attacaaatc aaaatttatt
      121 acatattatt tataaaaaaa tctaaggagg aaataataat gaaaatatta gtattaaact
      181 gtgggagttc ttcattaaaa tatcaattaa tagatatgaa taatgaagaa gttttatgta
      241 taggattggt tgaaagaata ggaatagaag gttctatatt aaaacatgaa aaagcaggta
      301 gagatgataa atacgttgtt gaacaaccaa tgaaggacca caaagatgca atagcattag
      361 ttttagaagc tgttgctcat ccagaatttg gtgcagttaa agaaatgaaa gaaatagatg
      421 cagtaggaca tagagttgta catgctggag aaaaatttgc aacttcagta gtaataactc
      481 cagaagtaga agaagcttta aaagaatgta tagatttagc tccacttcat aatccagcaa
      541 acataatggg aatagatgct tgtaaagcta tacttccaga tgtaccaatg gtaggtgtat
      601 ttgatactgc tttccatcaa acaatgccta aatcttcata cttatatggt ttacctcatg
      661 agctatatac taaatatggg gtaagaagat atggattcca cggaacttct cataattatg
      721 tatctcagag agcagcagag atattaggaa aagatataaa agatttaaaa atagtaactt
      781 gtcacttagg aaatggtgct tctatagctg ctgttgatgg tggaaaatgt gtagatacat
      841 ctatgggatt cactccatta gaaggattaa taatgggaac tagatgtgga gatatagacc
      901 cagctatatt accattctta atgagaaaag aaggtttaga tgctgatgga ttggataaat
      961 taatgaataa agaatctgga gtatatggaa tgactggaat ttctagtgac ttcagagata
     1021 tagaagatgc agctaaaaat ggagacgaaa gagctcaagc tacattagaa gcgtatgtta
     1081 aaaaagtaca aaaatatata ggcgcttatg ctgctgaaat gaatggattg gatgttgtag
     1141 tatttactgc aggcgttggt gaaaatggaa aggctataag agcagatatt gcttctaaca
     1201 tggagttttt aggaatgaaa ttagataaag aagctaatga tgttagagga aaagaaacag
     1261 taatatctac tgctgattct aaagtaaaaa tgcttttaat accaactaat gaagaattaa
     1321 tgatagctag agatacatta agattggtaa aataattaaa ttaaacctat gcctatcaag
     1381 taaaaatact tgacaaacaa atgctagatt tgtataataa atttggtgat atgttgaggt
     1441 gaacttaatg aaagttagca tagaaaaaat aaatagaaaa gaaactgaca aaatagactt
     1501 gaatttttgt gaaaaaattg atactattag ttattgtgat gagatttaca aattagtatc
     1561 acctgtaaat ttaaaaggga aagtgtcaaa gactaataaa ggtttgtatc tagatataga
     1621 tgtcaatttt acaattgttg acaattgttc aagatgtctt aaagaagtag aaataccact
     1681 agagtattct atacaaggtt ttttagtaaa agaagaagat tatgatgaag atgagtttga
     1741 ggaatttgat ccttttatat tcgatggtga agaaatcgac cttattgata taatagaaca
     1801 gacattagat tttaatgtac ctcataaagt tctttgtagt gaaaactgta aaggtctttg
     1861 tcaagtatgc ggggcaaact taaatgaaga agagtgttct tgcagtgaaa ttacaaacga
     1921 tgaggaatac atagatcctc gttttgctaa attaaaagat ttatttaatt aaattttgac
     1981 taaggaggtg gcgtaaatgg cagtaccaaa gcgtaaaacg tctaaatcaa acacaaaaat
     2041 gagaagagca gctaactcaa agatggaagc aacaggattt gtaagttgcc cacaatgtca
     2101 tgagccaaaa ttaccacata gagtgtgtcc agactgtgga tattataaag gtaaagaagt
     2161 tgtgtctaag taatagcttc taaaaagcat gtagagtata ctctacatgt tttttttgta
     2221 catataaaaa aataagatga tttactagag aaaataagtg gattttatgc atattaaaaa
     2281 atagaaaaat aataatataa gtattgaaaa tttttgtgtg ttactatata ctactattag
     2341 tagctggtac taattacagc tataaaattt ggagggaaca tgaaaaagaa aagtaaagcc
     2401 caaagacaaa aagagcttat agatatgcta aagacagatc ctttttatac tgatgaagaa
     2461 ttatcaagtc tgtttgatgt aagtatacag accataagat tagatagaat gagtctaaac
     2521 ataccagagc taagagaaag agtcaaatca atagcagaaa ctcagagttc aaaagtaaag
     2581 actttaggag taaaggaaat tacaggagaa atcattgatt tatctgtagg gcgattaggt
     2641 atatctatgt tagaagttac acaggacatg atttactcta agactaatac tttaaaagat
     2701 acgtatattt tttctctagc agattcattg gcaatggcta taatagatgc acctaaggtt
     2761 attatgagag ttgcaaatgt aaaaagtttt aagttgatag aacaacaaga tagacttata
     2821 gcaaaagcag aagtgtatag aaatatagat aaaaagcatt atgtaaaagt tgttatcaac
     2881 aataaagccc aagagcaaat atttagagga aagtttatct ttgaagaatt agattaggag
     2941 ggaaattatg aaaatagtaa ttgatggaat gggtggagat aatgcaccta aatctaatgt
     3001 agaaggcgca gtaaatgcta taaaagaata tcaggttgat ttaataatca caggagataa
     3061 agatttactt gaaaaggaat tttcaaatta tgaatttgat agaaataaat tagagatagt
     3121 tcatactact gagattatag aaaacgaaga taaaccagta aaggctataa gaagtaaaaa
     3181 agattcatct atggtagtag cattaaatct tgtaaaagaa ggaaaggcag atgctataat
     3241 atcagctgga aatacaggag ccttacttgc tggtggatta tttgttgttg gtagaataaa
     3301 aggaatcgat agaccttgct tatgttcagc aataccaaat gtaaagcgag gaatgacact
     3361 tatagcagac tgtggcgcaa atgcagattg taaacctaag aatctagttg aatttgcagc
     3421 tatgagtaat atttattcaa gaaaagtttt aggtcttgaa aatcctaagg tagcattagc
     3481 aaatgttgga ctagaagaag gcaaaggcaa tgatttagtc aaaaggtcat atgaagaaat
     3541 taaaaaatta gatttaaatt ttatagggaa tgtagaagct agagaagtta taaatgcata
     3601 cacagatata atcatatgtg atggatttac aggtaatata ttacttaaat ctgctgaagg
     3661 tgtagcactt tcagttatga gtcttataaa ggaaactttt atggccagta caaaaagtaa
     3721 aataggagca ttacttataa aggatgattt aagaaaatta aaaagtttta tagattattc
     3781 tgagtatggg ggagctccac ttttaggtct aaatggagga gttataaaag ctcatggtag
     3841 ttctgatgct aaagctataa aaaatgctat aaatcaagga ataaaatttt caaagggaaa
     3901 agtcgttgaa gatattaacc aatttataag caaatacaat gaagaaaata aaaataacga
     3961 agatgaataa actcgttttg gaggtttaat atataatgaa tacaaaggct ggaatattag
     4021 gagtaggaag ttatctacct gagcaatcat atgataattt tcacttcgaa aaaataatgg
     4081 atacatcaga tgagtggata agtactagaa ctggtattaa ggaaagaaga tttgccaaag
     4141 agtctgaagc aacttctgac ttagcatcta aagctgcctt aaaagctata gaatgtgcaa
     4201 agttaaacgt agaggatata gagctgatta tattagctac aattactcct gatatgtctt
     4261 tgccttcaac tgcatgtata gtacaagatg caataggagc agtaaatgct actgcatttg
     4321 atatatcagc agcttgttct ggatttgtat atggagtaac tattgcaaag caatttgtgg
     4381 aaacaggttg ctataaaaat gtgcttgtaa taggagcaga aacttgttct aagtttttaa
     4441 attatgatga tagaacaaca gcagtattgt ttggagatgg agctggagca gctgttattg
     4501 gccctgtaaa tgagggtgga atactgtcta cacatatggg ctctgatggt aaaggaaaag
     4561 attgtttaaa agtacctgct ggaggttcaa gacttaaggc aagtaaagag actgtagaag
     4621 ctaatctaca tacaatagaa atggcaggta gtgatgtgtt taaatttgca gttagaaaga
     4681 tggcagaaac ttctttaaga gctcttgaaa aagcaaatct aaatacaact gatatagact
     4741 acttagtgcc acatcaagct aatataagaa tcatacaagc ttcttctaag agattagagc
     4801 ttgatatgaa aaaagtttat gtaaatatag acaaatatgg aaatatgtct gctgcatcta
     4861 taccagtagc attagatgaa gcttatagag aaggtaaaat taaaaagggt gataatgtag
     4921 ttttagttgg ctttggtgga ggtctaactt ggggggcttc agttgtcaag tggactttat
     4981 agtataaaat atctaaaatg atttaattaa caaaattaaa cttatacaca aaataaagtt
     5041 gattaataat aaaaagttta cttttatgat ttcaagtgaa ggagaattgt atgaataaaa
     5101 tttgcaaaat attaaatata aaatacccag ttatccaagg gggaatggca tgggtagcta
     5161 ctgcatcatt agcaagtgct gtatctaatg caggaggact tggcataata gcagcaggaa
     5221 acgcaccaaa agaagctata aagaaagaaa ttgttgagtg taaaaaatta acagataaac
     5281 cttttggagt aaatgtaatg cttatgtcgc catttgttga tgatataatt gatttgatta
     5341 tagaagaaaa agttcaagtt attactactg gtgctggaaa tcctgcaaag tatatggata
     5401 gattaaagga agctggaaca aaggttattc ctgtagtacc tacaatagct ttggcacaaa
     5461 gaatggaaaa gctaggagct acagcagtaa tagcagaagg tactgaaggt ggaggacata
     5521 taggagaact tactactatg gtcttagttc cacaagttgc tgatgctgta aacatacctg
     5581 taatagctgc tggaggaatt gtagatggta gaggaattgc agcatcattt gcattaggtg
     5641 ccagtgcagt tcaagtagga actagattta tttgcagtga agagtgttct gtccattcaa
     5701 actataaaaa cttagtacta aaagcaaaag atagagatgc aattgtaaca ggaagaagta
     5761 ctggtcatcc agtaagaaca ttaaaaaata aactatcaaa agaattttta aagatggaac
     5821 aaaatggagc tactcctgaa gaactggata aaaaaggtac aggagcttta agatttgcaa
     5881 cagtagatgg agacatagaa aaaggttcat ttatggcagg tcaaagtgct gctatggtaa
     5941 aagaaataac accttgtaag gaaattatag aggctatggt aaatcaagca agagagatta
     6001 tgccagcaat agaactgtaa atcaggagga gcaagatggg aaaagttgca ctagtattcc
     6061 ctggtcaagg agctcaatat gtaggtatgg ccaaggattt atatgaaaat aatactgttg
     6121 ctaaaagtgt aatagatgag gctagtgatg ccttaaatat ggatttaaaa aaccttatgt
     6181 ttaatggaaa tgaagaagaa ctttctaaaa ctgaaaacac tcaacctgct atggttacac
     6241 atagtgtagc tgtattaaaa gcagtacaag cccaagtaga tttaaaatat agtgcatgtt
     6301 taggtttatc tttaggagag tatagtgcat tggttgcagc agatgcaatt gactttaaag
     6361 atgctgtatg cttggtcaaa aaaagaggta agtttatgca ggatacagtt ccaacaggtg
     6421 ttggagctat ggcagcaatt ttaggtctag atagaagtat attagaaaat gtagttaaag
     6481 atatacaagg aggtattgtg gaagtagcaa attataattc tcctggtcaa atagttatat
     6541 ctggtgaaaa tgaaaagata gaagaagcaa tggtgaaatg caaagaagca ggagcaaaaa
     6601 gagctgttaa attaaatgta agtggacctt ttcactcatc aatgctaaga gaagctggtg
     6661 taaaattggc cagtgaatta gaaaaagtta acataactaa acctaaagtt gatgtggtgg
     6721 caaatgtaaa tgcagattac tataaaaacg aaggtaaaga cctgcttata aaacaagtaa
     6781 gttcatctgt tctttgggaa gattctatag aaagactttt aaatgatgga tatgatactt
     6841 ttatagaaat gggacctgga aaaactttaa aagcatttat taagaaaata gcttccaata
     6901 aaaaagcaaa tgtcaatatt tataatatag atggtataga ttctcttaat gaatttgtac
     6961 aaaattatag aaatggagag atataatgat aaatcttaca ggacaagtag cagttgttac
     7021 tggtggctct aggggtatag gaaaagaaat agcaaaaaaa ctagcatctt ttggagctga
     7081 tgtagtaatc aattatactt ctaaagaaga tgaagcacta aaaactaaaa atgaaataga
     7141 aagtatgggg gtaaagtgta cctctataaa atgtgatgtg tctaaatttg atgaagtgaa
     7201 tcaaatgata gattctgttg taagcgaatt tggaaaaatt gatatattgg ttaataatgc
     7261 aggcataact aaagatggtc tgcttatgag aatgaaagaa gaagattttg atagagttat
     7321 agatataaac ttaaaaggtg tctttaattg tacaaaagca gttactaaac ctatgatgaa
     7381 aaagaagtat ggaagaataa taaatatgac ttcagtagtt ggaattatgg gtaatgcagg
     7441 gcaaactaat tattgtgcat caaaagcagg tgtaattgga tttacaaaag cttctgcaag
     7501 agagttagca tcaagaaaca taaatataaa tgcagtagca cctggattta tagaaacaga
     7561 tatgactaaa gtactaagtg atgatgtaaa agaatcaaca ctagcaaaca taccaaagaa
     7621 atcttatggt aaaccagaag atgtagccaa tgccgtagca tttttagtta gtgacatgtc
     7681 aagttatata acaggacaag taataaatgt agatggtgga atggtaatgc aataataatt
     7741 taaaaagtag agaaatctat taaaataaaa aattttagga ggtacacaat gtttaataaa
     7801 gttgtagaaa taataataga gcaattagga gtagaagata aagaaataac tatggaaaca
     7861 tcattaatga aggatttaga agcagattca ttagatgcag tagaaataat aatggcactt
     7921 gaagatgaat ttggtataga aataccagat acagaagcag aaaacttcaa atctatagga
     7981 gatatagtta agtatataga agctaataaa tagtcgagct ttaaaaaagt ggaggtatcc
     8041 gatgaataaa agagtagtaa taacaggact tggatgtgtt acgccgttgg gaacaggaaa
     8101 agaagagttc tggagtaata taaaatctgg agtatctggt atagataaaa taactaattt
     8161 tgatgctagc acatatcaaa ctcagatagc aggagaagtt aagaattttc atccagaaga
     8221 atatataagt aaaaaagaat tgaagagatt agataaattt gctcaatttg caatagtatc
     8281 tgctaaatta gcagttgaag atgcaaatct tgatttagat aaagttgata gagaacgttt
     8341 tggtgttata ataggttctg gaataggtgg agttgaagct atagaaactc aacataaaat
     8401 tcttttggaa aaaggaaata aaagagtaag ttcacttttt gtacctatga tgataggaaa
     8461 tatggcagct ggtcaagttt caatattttt aggagcaaaa gggcctaaca ctaatgtatg
     8521 tacagcttgt gcatcaggaa ctcattcaat aggtgatgct tttaaggtaa ttcaaagagg
     8581 tgatgcagac atcatggtag cagggggttc tgaagctgct gtaacaggtc ttgcttttgc
     8641 tggattctgt aacatgaagg ctatgtcaac tagaaatgat gaccctaaaa ctgcatcaag
     8701 accatttgat aaagacagag atggttttgt aatgggagaa ggagcaggta ttgttatctt
     8761 agaagactta gaacatgctt tagcaagagg cgctaaaata tatgcagaag tagttggata
     8821 tggtttaact gctgatgcat accatatgac aactccagct gaaaatggag agggagcagc
     8881 taggtctatg aatatggctt tgaaagatgg aaatgttcca ttagaagaag tagattatat
     8941 aaatgctcat ggaacttcaa cttattacaa tgatttatat gaaactatgg ctataaaaac
     9001 tgtatttgga gaaaaggcat atgacttatg tgtgtcatca acaaaatcta tgactggtca
     9061 cttattagga gcatcaggtg ctatagaggc tgttgtttgt gctatgagta tagaagatag
     9121 ttttgtgcct ccaactataa acatacaaga agttggtgaa gatttggatt tagattatgt
     9181 tccaaatcaa ggtaaagaaa aaaatattag atatgcacta tcaaattctt taggatttgg
     9241 tgggcataat gctacaatag tactaaaaaa atatgtataa ataaatatta aatataaaac
     9301 taaaaaacaa gcacttgttt taactgtata aatttatatt agcttaaatt taaagtctcc
     9361 taaattattt taggagactt ttttattaag atttaagtct agcatatgtt gtaaatcatt
     9421 ttgatatatt gtattattaa ttattaagag gttatttata tatgtaagtt ttcattctac
     9481 aatatacaaa aggaggacta aaattttgaa ttttagacta ccaaagaaaa ggttacccaa
     9541 aaagataatt tttatatgta taatacttat tagtattatt ttgtttataa agcatgacca
     9601 aaatggattg aaagaaagag aaattataaa taagggaaaa tatatgtctg aaagccttgt
     9661 ggcagataag gagtatgatg aagcaaaaaa agtggttaag acttctttcg aatctattcc
     9721 agaaaaaaaa tacaataaag tatataatga agtttgggat atgttaaaga ctatttcata
     9781 tgttccagat ggaacgaaga tagtcattga ttctttagaa aagatgagag aatcagatgt
     9841 gaaattatct gatgaatgta gatttaatat agaaaaaaga ttagcatcag tgtatataat
     9901 ggataataat tattctaagg cagtagattt aactgtaaag tctataaagt tggctgagaa
     9961 attggggaat aattatgaaa aagcaagact agatgttgat ttggcgagca tatttttaaa
    10021 gataggtggc tatgaaacag gagagaaatt aataaaggat tcttttaaaa tagatattga
    10081 tgaaggtgaa aaaaatggaa tggttagatt atatgctatt ataaatctag cagaaatata
    10141 tgtagaagag ggcgaatatg ataaagctat tgaaataagt agcaggataa aagattataa
    10201 aaagtttgta aatacggata attacaatga ttttgatata atggcctcta taatgcagtc
    10261 aaatgcgtat gtgggcaaaa ataatataca aaaggcaaag acttttttag aaaaagctga
    10321 ttatcttata aaatcagata gaacagttta tatattagat aaagatatgt actattatat
    10381 ggcaatggga aacttagaat ataaaagcga aaattataat tctgcaatag ataattataa
    10441 aaaaagttta gagatatcta ctaaaagaaa gttaacccag gagaaaataa aaaatttaaa
    10501 taagatatta aatatatatg aaaaacaagg aaatatagaa tctttaaatg agtatcaaaa
    10561 gatgttaata aatgaatata aagagaacaa aagtataaga gattcagaga ctagtttcta
    10621 tataattgat aaagtatcaa acgaaagtaa gctatttgaa aaaactaaaa aagaaataaa
    10681 atattataaa atattattta cagtaatttt aattgctata ttagggtcta cttttttata
    10741 caaccgattg aagtatttta aagcacaaaa tttacatgat ggtcttacaa atgtttataa
    10801 tagaaaaagt tttgatatta tgtatgaaaa gtacagagag aaagatgata attttgcact
    10861 tgtgatgata gatattgata atttcaagct cataaatgat aattatggac ataaatttgg
    10921 tgacactgtt ataaagggaa taacaaatac catttgtgtt atgttagaac aagatgataa
    10981 agtctttagg tacggaggag aagaattttc tgtattaata agaaataaat ctggtgatga
    11041 agtagcagat ataattgaca gtataagggt tgcaattgca aataaaaagt ggaatgatga
    11101 tgtgactgtg actataagtg caggtgtagc tcatgtttca gatagcaaag atgtactaga
    11161 agaagctgat aaaaatttat ataaagcaaa acaacttggt agaaataaag tagtatataa
    11221 gtaattatat actatataaa taagaggtat aaatatgaaa caatcaatag ctatagtaac
    11281 tgataaaata actaaactat catctttctt agaagaaaat atacgtttgg ttttaggtaa
    11341 ttatattgat ataaaccact attatctgga agttttaaaa gagaatgata aaatacaagg
    11401 tgattttgta ctagtaatga atgatgatag attaaacagt atgagaaagc atttagtaca
    11461 agatagtaag attatagtga taaggagaac acttaaagaa aatgaaatat atggattatt
    11521 ttcaatacca aatggaactg aagtattggt tgtaaatgat acaaaagaaa ctaccttaga
    11581 aacgataagc ttattttaca aaataggagt aaaaaattta aagtttactc catatattga
    11641 aggaaaaatt tataaaaata tagacatagc tataacgcct ggagtaaagg gaaaagtacc
    11701 aagttatata aataaagtaa tcgaccttgg aaatagatat attgatatat ctacctttat
    11761 agaaataata aatggtctta gaatagatgt taaagaaata cgtaaaaatt taatggcata
    11821 ttctgagagt ttaataagtt tagataatgg agttaaagat aattataggc aactgttttt
    11881 aaaaatagaa gaacaagatg ttatattgaa tctatcaaaa gatggaataa tatttacttc
    11941 agcagatggt ataataaaca catttaataa agaagcttta aaaattttag gtataaatga
    12001 aaatccacaa ggcaaaaaca tagaagatat catttctgaa gatttaaata ttttacttga
    12061 agacaaagaa ataattgatg aagtggtatt tacaaataaa aaatatataa atgtaaataa
    12121 aaaaagtatt ttttctatgg ggaataaagc tgggatatat tataacttac aagaaataac
    12181 gtatataaaa aaattagaac aaaatcttac taataaatta agagaacaag gtcagatagc
    12241 aagatataca tttaaagata taaaaactaa aaataaaaat atggtaaagt gtgtagaatt
    12301 ggctaaaaaa atatctaaat cagaactttc tgtattaata ataggagaaa gtggtactgg
    12361 taaagaatta ttatcacaat caattcataa tgcttccaat agaagtaatc aaccgtttat
    12421 agcagttaat tgtgcagcag ttccagatag tctattagaa agtcaactgt ttggatatga
    12481 aaaaggctct tttacaggag ctttaagaga aggaaaaaag ggtctatttg aacttgctaa
    12541 taatggaact atatttctgg atgaaattgg agatatgcct ccattgctac agacaaagtt
    12601 actaagggta ttacaagaaa agcaggttat gcctataggt tcccataatg ttataaacat
    12661 agatgttagg gtcatagcag caacaaacaa aaacttacta aagatgatta gagaaggaaa
    12721 atttagagag gacctgtatt atagactaaa tgtattgcca atagatgtac catcattgag
    12781 gaatagaaaa gaagatataa taacacttat gaatttcttt ttgaaaaaca atataagact
    12841 ttcgccagaa gttataacta ttttagaaaa atataattgg ccaggaaata taagagagtt
    12901 acaaaatgtt gcttcttatg taagtctaat gtgtgatagt atggttttta gagatgactt
    12961 accaccatat ataagggtag aatgtgctaa caattatgaa gatggatttg gttatacaag
    13021 aaaagaattt gaggaaatac tgaggattct atatgaaaga agagagtcat cctctggaat
    13081 tggaagaggg tttataatcg aaaagcttat acaggaaggt attgagattt ctgaaagcaa
    13141 agttagaaag gtgctatctt atttaagtga atgtggatac atatcatcta aatctggtag
    13201 aggaggaagt caaataacct cagaggggat aaaactttat aataatataa attaagctaa
    13261 gtatttgata ttgaaatcac atattttgaa tttagatatt taaataataa taaactttta
    13321 tatttataga gattaattta tattatgtca gaaagaatat tgtaatttat aattaaatat
    13381 taataggttt ttaaaatagg attagatagg taataggtta agttagaata acccattacc
    13441 tatttttatt tgtaagaaaa gtgtacatag aattagaagt aaaaaataca actaataaaa
    13501 ctattgaaaa tactagataa ataataaata aaaataatta tagattttgg catacatatt
    13561 gctaattata gagataagac gacaaaataa ttgggaggtt taatgatgaa atacaaaaca
    13621 acagggactt gtgctacaga aattgaattt gaagtaaaag aaaataaagt tacaaatgta
    13681 aactttatag gtggatgtga tggaaattta aaagggctaa aagtattagt tgaaggcatg
    13741 aatatagaag atgtaataca aaagttaaaa ggaatagagt gtaaaacaaa gccaacatct
    13801 tgcccagacc aactgtctct agcattggaa aattatatga atataaagta atacataaaa
    13861 caatcgatta gttaggagtt taattatgaa accaataata gggattttag gaaatttaat
    13921 aataatggaa aatggtatgt ttccaggtct tgaaaggtca tatgtaaata atgattatat
    13981 aaatgcagtg ctaaaaggag gaggtagtcc agtaataatt cctgtaaata cagataaaga
    14041 agttataaaa aaacagattg aaatggttga tggagtctta atatctgggg gatgggatat
    14101 caatcctcaa ctgtatggag aagaaacaag agaagaaacg acatttatat atccagaagt
    14161 ggatgaattt gatttaattg caataagtat agcattagaa cttaaaaagc ctatattagg
    14221 tgtatgtagg ggactacaaa ttttaaatgt aagtttagga ggaactttat atcaagataa
    14281 taatcttata gaaggaagtt atataaaaca tacacaatct tctaaaagac atgtagctac
    14341 tcataaagtt gatgttaaag aaggaagtat tttagaggga attttaggaa aacaattact
    14401 tacaaatagt tatcatcatc aaagtgtcaa tcagcttgga aaaggattaa aagccatagc
    14461 atactcaaaa gatggtataa tagaagctat tgagaaagag gatgaaaatt ttgtagtagg
    14521 agtacagtgg catccagaaa tgatggtaga ttactgtgat aaaatggaaa aactatttaa
    14581 atattttata aatatttgtt caaatttata atataccttg gggggattga acatgaacga
    14641 aaagaacaaa atgggattaa taagtattat acttttaggt attaatgcag tagtaggagc
    14701 tggagttttt ctattaccag gagatgcaat gaaatcattt ggtgtagcaa gtatatttgt
    14761 atatatattt gatatgttgt tagttttatc tatggcattc tgttttgctg aggttgcagg
    14821 taaattcaat aaaaatggtg ctgcctatgt atatacaaaa gaagcttttg gagatttttg
    14881 tggttttgaa gtaggtctta tgaaatgggt aataggatgt atttcttggg gagctttaat
    14941 agtaggtttt cctacatcat tatcagcagt atgggctcca gcaggtgaac cacatataca
    15001 aaaaattata attgtagtca tgatagttgg gcttacaatt ataaacttat taggtgtatc
    15061 tctatcaaaa attgtacaaa atgttataac tgtaggtaaa ctaattccat taatactatt
    15121 tataggaata ggtatatttt ttataaaagg agttaatttt acttcttcaa caatggtacc
    15181 tccaggagct ggagcaacag agtttggagc agcagcactt ttaatgtttt attcatttac
    15241 aggatttgaa tctatagcag ttgcagcaga agatatggag aatccacaaa aaaatatacc
    15301 aatagcaatt ataagtgtaa tagtaattgc atcaataata tatatattaa atcaagttgt
    15361 atgtgttggt atactaggag attcgttatc tagtacatct acaccagtgg cagatgctgc
    15421 aagaatatgc tttggaaata tgggagctgg attggttacc tttggaacct tggtttctgt
    15481 tggtggaatt tgtatgtgtg gtgcttttgt aaatccaaga agctgtgtag ctttagctga
    15541 tgataaaatg ttaccgagaa tatttgcaag aaaagataaa aaaggtacac catatgtagc
    15601 gataatagct actatgctaa tcactatacc aatagctcta tctggaagtt ttgctgaatt
    15661 agcagcaata agtgcagttg ctagattcat acaatacata ccaacatctt tatccgtatt
    15721 agtgtttaga aagaaaagac cagaattagt aggtactttt aaaactcctt ttggagcagt
    15781 gattcctcta attgctgtat gtgttggaac atggttatta ttccaagcat ctatgcatca
    15841 attaattatg ggattaggag ctttggcaat tggagtacca ttatacttta taatgaagtc
    15901 ttataataga aaagtctatg atgaaaattt aaagaaaata gtttaattat gttataacaa
    15961 tttattgagt taaaattaga cattggcata aaattaaata caaataagta tctacaaggc
    16021 tatattaaaa tattattaat tttttatttt aatatagcct tatattgtta tcaataaatg
    16081 aaaaatattg taacaataga agaacaatag tattatgttt atagacaaaa aacttattag
    16141 gaggtaaatt tatgccaaca ataacattaa aaaatggtgt agacgtactt attagagaag
    16201 gtgtaagaga agatgctcaa agcattatag atttttataa tgaagtgggt ggagaaactc
    16261 attttctgtc atttggaaag gatgaatata aaatatcttt ggaggaacaa gaaaatgcta
    16321 tagaatcagc aaaagcatcc gataattctg ttaaactgat tgcatttata gatggtgaaa
    16381 tagttgggat tgcaacgatt gattctaatc aaaaagcaaa aggaaagcat gttggagtat
    16441 taggaattgt tgtaaaagaa aaatattggg gaattggatt gggtaaaagg cttatgttag
    16501 accttataga atggtgtaaa tcaaatggaa taaccaagaa gataactctt gtcactaatg
    16561 aagaaaatta taatgctata ggattataca aaaaagttgg atttgaagta gaaagcatac
    16621 taaagaaaga atgttattat aatggtgttt atactgattt aataggaatg tcacttttat
    16681 tgggaatata attagtgtac attttatctc gaaaaaagta tattaagtat ggtataatta
    16741 aaaaagaata taacttaata taattttgga ggacgaatta tgaaaaatct gtgtaaaata
    16801 tcagatgatt atttaaacag caagcttaag tatttaaagt tattatcaaa acaatatcca
    16861 agtatatcaa aagcaagtac agaaataata aatttagagg ctatattaaa tcttccaaag
    16921 ggaacagagc attttataac agatgtacat ggtgaatatg aaccatttgt acatgtatta
    16981 aaaaatggtt ctggtgtaat aaagagaaaa atagaagaat tgttttcaaa tacaataaga
    17041 gacagcgaaa aaaaaatgtt agcaactctt gtatattatc ctgaacaaaa gttagaccta
    17101 ataattaagc aagaggaaaa tatagatgat ttttatagaa taaatattta tagacttata
    17161 gaactttgta aatatgcttc tagcaaatat actaggtcta aagttagaaa actgttacca
    17221 gaaaatttta aatacataat agaagaactt ttgcatgagc atgttaaaag tgaacataaa
    17281 gaagagtatt ataaaagtat agttgaaaca atagttgaca taggaatagc taaggagttt
    17341 ataattgcta tttctacagt tatacaaaaa ttggttgtag atagacttca tgtaatagga
    17401 gatatttatg atagaggtcc aagaccagat ataattgtcg ataagcttat tgaacatcat
    17461 tgtgtagata ttcaatgggg aaatcatgat atattgtgga tgggtgcagc ctcaggtgaa
    17521 aaaacttgta tagcaaatgc actgaggata tcagctagat atgcgaattt agacattgta
    17581 gaagacatat atggaataaa tctattgcct ttagccactt ttgctataga aatgtataaa
    17641 gatgaccctt gtaaagaatt tattccaaag gttaatgacc aaagtgtaac aacaacagaa
    17701 aagtctttaa tggcaaaaat gcacaaagca ataagtataa ttcaatttaa gcttgaaggt
    17761 gaggttatca gacgaagacc tgaatttgaa atggaacata gactcttgct taatatgata
    17821 aactatgatg aaggtacgat taatttaaaa ggtaaaacat ataaattaaa agatacttat
    17881 ttacctacaa tagacaaaaa agacccatat aaattaacta tggaagaaag aaatgttatt
    17941 gataaattag tatcttcatt tagaggtagt gaaaaattac aaaaacatgt ttcatttttg
    18001 ttttctaaag gaagtatata tttaaaggct aactcaaatt tactgattca tggatgtgta
    18061 ccgttaaatg aagatggaag ctttatgtct atgaatataa tgggaaaaga gtataaaggt
    18121 aaggctctta tggataagat ggagtctcta gcaagagaag gatttttctt taaagataag
    18181 gcagaagaaa aactgtatgg catggatatt atgtggtatt tatggactgg gaaatgttca
    18241 tcattatttg gtaaagatga tatgactaca tttgaaagat actttatagc tgaaaaagag
    18301 actcataaag aaaataagaa cccatatttt aaacttagag aaaatgagat ggcttgtaaa
    18361 agattatttg aagaatttga tttagaactt gatgaatctc atataataaa tggtcatgtt
    18421 cctgtggaga gtaaaaatgg agagagccca ataaaagcta atggtaaaat tctcgttata
    18481 gatggtggtt tttctagggc ttaccaaaaa actactggta tagctggata cactttgata
    18541 tacaattcta gaactttgca attagtatcg catgaaccat ttaattctgc tgaagaagcc
    18601 attgcaaatg aaagtgatat cttatcgaca actgttgtag tagagcataa ggcaaagaga
    18661 aaaatggtga gagatactga tgaaggagta aagatacaag aggaaataga agatttaaaa
    18721 ctacttttaa tggcttataa gaaagggcta ataaaagaaa tgtagatgtt atagaggcac
    18781 ttcatgattg aagtgtcttt ttgttaatac ttattaaata tattataaag attaataata
    18841 gtagtttata ttatacttac taagtgaatc caagataaat gggttcactt tttttattta
    18901 taaacattat taaaaaaata tagatgtttt ggttatattc cttgtccttt ataaatagaa
    18961 ttatataaac aaaggaggag gacaagagat gaataaactt tctgatgaaa taataaactc
    19021 tctttctaca actataagag aaaaggtaga aaaagtctca aacaataatc tgaacataga
    19081 agaaattagg ttacgctcac aaaaaccatt gatactaaat gcaaactcta aagattattt
    19141 ttacaatcaa aaaactatga cacttgattt aaatcaacaa aactcatatg tagtaacaag
    19201 agaagatgta gagcaaactt ttcaaataat atgtaaatat tcaatacatt catttatgga
    19261 tgacataaaa aaaggattca taactttgag aggaggtcat agagtagggt tagttggaaa
    19321 agcaatagta gaagatggac aagtcaaaaa tataaagcat atatcatctt taaatataag
    19381 ggtatcaagg gaaattattg gatgctcgga taagatttta agtcatatca taaagggaaa
    19441 aaatcagatt aataatactt tgataatatc accacctcaa tgtggaaaaa caactttaat
    19501 aagagatata gtaagaaatt taagtaatgg taatgaagat tatggattta aaggtttaaa
    19561 agtggcttta gtagatgagc gtaatgagat agcaggagct tatctaggtg ttccacaaat
    19621 ggatgtggga ataaggactg atataataga aacttgccca aaagacttgg gtattacaat
    19681 gcttttaagg tctatgtcac caaatgttat tgtgacagat gaaattggaa gtgaaaaaga
    19741 aataaaggca ttatacacag cattaaatgg aggtatagga ttaataacta ctgtgcatgg
    19801 agattcaata gaggatatac aaaatagaaa agaattaaat agacttctgg ataaagagtt
    19861 atttaaaaag gttataattc tttctgcaaa aagaggagca ggaacaattg aaaaaattta
    19921 tgatttagaa gaaaagagat ggtattttgc aaattaaaat aattattata gctttcttga
    19981 taggaagtag ttatttgata ggtgaacaaa tatacaaaac ttatactcgt agacataaac
    20041 aattaaacga tttaataaga gttttagaaa ttttgaggat ggatttatct tttggacttt
    20101 atacattaga agaaatcttt aataggatag gtggaaataa agagttttgt ttttggaaat
    20161 ttttctatca aatatcagaa ggacttcaca atgaacaaag taaaactttg gaaataataa
    20221 tatcagaaaa tattgatgtc ttaagtaaag aaacttatct aggcaataaa gaaattgagg
    20281 aactgaaaaa tctaatactt actttaggga aaagcgatat agagtcacaa caaagaatga
    20341 tagatttatc tatagaaaat ttgaaaaaac aaacctatga gacaaaggaa gatataaaca
    20401 aaaaaggcgt attgtataaa aaattagtga cattcatagg tataggtata tgtattatat
    20461 tgatttaata tggggaggtt tattatggaa atatcattga tattaaaggt tgcaggtgtc
    20521 ggtattctaa tatcagtatt gaatatgata ttggaaaaaa cagatagaaa agattgggca
    20581 ggtcttacta cattagcagg tgtaataatc gtattgggta tggtaataac agaaataagt
    20641 gacttgttta atactgtaag aacaatgttt caactttact aaagaggagg gagtaatttg
    20701 gaaataatgc aattaatagg aattgcaatc atttcaacta cactctgttt agtaattaaa
    20761 aaagatagac cagagatagc aaatttcata gctataataa ctggagtaat aatactattg
    20821 tcagttatgt ttaagctaaa ttttatagta gatagtatac aagatttggc taacaaggct
    20881 aacatcccga ctatgtatat atcattaata ataaaattaa ttgggatagc atacttaatg
    20941 gaatttgcga tacaactttg taaagattgt ggggaaggaa acattgcttc aaaactagaa
    21001 tttggaggta aaataatagt tatgtcaatg tctttcccta tacttttgtc cattgtagaa
    21061 atggtggtga atataattcc atagatgtgt atcttggaat gaatataagt atattagaga
    21121 gcttttgttg tattgaggtt tatagagtga agagaggcat ggttgcttat gaaaaaaaga
    21181 tttttaccaa tgattatagg ttttttattt actttctttt ttgtaaatat ctttgctatt
    21241 atgatatttg ctaatgaagt accaagtagt gaagataaag aatatggtga aacaaaaaat
    21301 agcatagata agtatataga tggtcaatta gacaagctag atataaatga gattcaagac
    21361 tatataaata aagaaatagt tattaatgat gttaatttaa aatcatttgt aaaagattta
    21421 attagtggag aaaaaaatat cttagattta ttcaataaag atggattgaa gatattgatg
    21481 tttgatgaat ttaaagcaag tttaaaggta gtagcagtaa ttttggtact ggctctgtta
    21541 tcatctattt taaagagtct ggaaaactct ttttcatcag gtgcagtaag tcaaatagca
    21601 acatatataa tatttatcac aatggtatca cttacactta ttggttttaa agatgtatta
    21661 cagatttgct atgatgcaat agaccatact gtaggtctta tgcaagttat aatgccaata
    21721 ttaataacat ttttactttt aattggattc ccaataacgt cgactacttt gaatcctatc
    21781 ttcataggag gagtaacatt tataaatgta ttttttaaaa attttctatt tgtatctatt
    21841 actgttgcat ttggaatttt aataataaac aatttatcaa aaaatataag gctaaagaga
    21901 tttttctcct ttgtaaaaca aataaattat gtttctattg gagctatgtt tactgtttac
    21961 ttaggtcttg tatccataca aggattgtat gtaaccagtt ttgataaatt cagtgtaaag
    22021 actgctaaat ttgctatagg taattttatt cctgttgtag gtggatttgt atctgactct
    22081 gtggatattt tattatcttc atctcagctt ataaaaaata tatttggagg aatagggctt
    22141 atcttacttg ttggaatttg cttacttcca gtaataaaaa ttttatcagt aatagtagtg
    22201 tacaaattag cagcaataat tgtagagcct gttggagagg atggaatatc taatttttta
    22261 aatgaagtgg caaatttaat gataataatg ctagcatcag taattgctat aacagtaatg
    22321 ttctttgtaa ctgtagctat cttgacctcg ataagtgtcg taagtcaagg gtaatttata
    22381 tatcagtgaa aaataattac atttaatcta atgtttttag agaggaggat gctatgttag
    22441 agggcataaa ggcatggata gttagtgttt tgataggagc atttatagta aatatagtag
    22501 atatgatttt accaagttct aagataaaac cttatgtcaa cctagtttta aactttatgt
    22561 ttgtatttat tgtaataact ccagtagtag gatttttttc aaaagatatg agtttagaag
    22621 atagaatatt aaaatctatg ggaaactaca acaagcagta tgtagatagt acaaatgctt
    22681 tggcaaaaga gactggaaat aatagtctat ccaagggata tgaggatggg ttaaaagaag
    22741 tacttaaatt aaaacttgat gaatatggtt atgatttaga agatatagag cttaatgggg
    22801 caaatataaa taatataaaa ataaaagaaa agaataatag tgcaaaaagt aataacagtt
    22861 ccaatgaaga aaataaaaat aatagtacta aaagagagga agaaaacata aactctaatg
    22921 acaaagaaaa ttctaaacaa gtcttcaaaa aaggtacaga gtatgggttg aacctaaatg
    22981 aagaaaaatt aaaaaacgac ttaataaagg tgctggatgt ttctatagaa gatatacaaa
    23041 ttgataaata ggagttggga gaatgttgaa aaacttaaat gacaaagata agagaaaggt
    23101 atattcacta ataactatag caggaatatg tgtggtttca ttagtattat tatcttgttt
    23161 tcctagtagc aaaagcgata aacaagttgg aaaaacagag actaataaaa atgctgaaaa
    23221 gcaagtcact aaagaacaag aaaaagatga tttagagtca aaattaacag caatactatc
    23281 taaaattgat ggtgcaggag acgttgatgt aatggttaca tttgaatcaa gtgaagaaat
    23341 acaacctgct tttaattcaa ataatacaac agaaacaaca gaggaaaaag atgcacaagg
    23401 tggagaaaga acagttacga cttctagtga aaataaaaca atgataactt caaattcaag
    23461 tgaccctgta gttataaaaa ctacagagcc aaagataaag ggagtgatag tggtagcaag
    23521 tggagcaagt gacccaagtg ttaaagagac actttatagt gcagtacaaa catcattaca
    23581 agtagctggt catcaagtag aaatatattc taaataaata catataaccg gggggataaa
    23641 tgaatgaagt ttaattataa gggaagagga tttgtaataa taactttaac tgcaatgtta
    23701 gtggtagtag gaacagtgaa ttatcaacta agtaagaaat ctttattaga aacatcaaaa
    23761 gagtttaagg catatgaaga agcacagttg caaaaaaata ctgatgatag ttatagtagt
    23821 aagacagagg attctagtaa tgtagataaa caaggtggta aggaaagtgc tgatattgat
    23881 atagtcgata gcaaagctag taaagtaaaa gaaaaggcaa ctgagacaag taaggaaata
    23941 aaagctcaat tatcatcaga gaaaaatatg aaaaaggcat cttatatttt agatatgaaa
    24001 atgaatagag aaaaacaaag aaatgagtta gttcaagatt taaatgaaat gataaataat
    24061 ccatctacaa cagaagaatc tagaaaagaa gcttctaata tgaaattaaa tatagttaaa
    24121 attcaagaaa aagaacttca aatagaaaac cttttgagta caaaaggata tgaagaagcg
    24181 ttagtttata taagcgataa caaagttaat gtagttgtaa atgaagctaa attagaaaag
    24241 aaagatgcag caaaaatatt tgatttagtt gcagaacaag ccaatgtgaa gtatgaaaat
    24301 attaaactta caaataataa tagtaataag taaagtataa acctcagaat taactgaggt
    24361 tattttcttg caaagtagac ttgtatttgg tattattata cttataaaaa tagttcctta
    24421 aaatcaggag gtaagtttta atatggaaga taataaattt ggacaagtaa aaatatctaa
    24481 tgatgtaata gctacaatag ctggtttagc agcattagaa gttgaaggca tagaaactac
    24541 agcaacatta acagataagt tgttaaaaaa taatggagta aaaatacaaa tcgaagaaga
    24601 ggatgttaat ttagatgtaa tggttacgat aaagtatgga atgtcaatac cagatacagc
    24661 ttttaaagtt caagaaaatg taaaaaatac tgttgagaca atgacaggac tgaaggtttc
    24721 tcaagtaaat atacacattc aaggaatcag ctttaagaag gataaagttg ataaagaaga
    24781 agcaaaagca actaagaaaa attaatatta gccctctgaa aagtcagagg gttttttgct
    24841 ggtaattgat tttatatgga taattaaact taaaggagaa ttttgattga tgaaaaaaga
    24901 tagggcacaa aagagtacaa caagagaata tataatgaaa cttatatacc aaataaatat
    24961 taataaagaa gattttgaaa ctttagaaga taaagtagat aattttttaa aagataattc
    25021 agaacatatt ataaatagat ataaagaact tgcacttcaa tactcaaaaa atacaaattt
    25081 aaagttagaa gatactgaaa ttgaagatgt tatagacaaa aaatatataa atacagtatg
    25141 caaagcattg aaagaaaatc atgacaaaat agatgagtta ataaataagc atgctaaaaa
    25201 ctggacagta gatagaatgc caaaggtaga tgtttctata cttagattgt ctgtgtgtga
    25261 aatactttat ttagacactc ctaataaagt atcaataaat gaagcagtag aacttgcaaa
    25321 aatatattgt gatgataagt ctcctaaatt tataaatggt atattgggaa gtgttgttga
    25381 tgaaattggg aaataatata ataattggaa ttgatactag ctgctatact acttctatag
    25441 cagctatctc tttagataag aaagttatat tcaatgaaaa aataatgtta gaagtaaggg
    25501 ataattcaaa aggtttaagg caaagtgaag ctgtatttca acatatcaat aatttaggga
    25561 ttcttagtga taggataaaa tcttttaaag ataagtttaa tgtagaagga gtttgctcat
    25621 caaaaaaacc gagacctgtt gaaaactcat atatgcctgt attcaatgta ggtcataatt
    25681 ttggaaaatt attgtctagt atatatggtt gtagatttta tgagactact catcaagaga
    25741 atcatattga agcaagttta ttaaatagta aattaaaaaa taataataaa tttatttctg
    25801 ttcatatgtc tggtggtact acagagatac ttttaacaag caaacaagat agtcatcaca
    25861 atgtatgtga tactaattta ggtaaaattg ccaaaataag tattaaaaaa gatgataaaa
    25921 gtaagttata caataatttt ggatataata tagatataat tggaggaagt aaagatatta
    25981 gctttggtca acttatagat agagtaggaa ttaagcttgg atataaattt ccttcaggaa
    26041 agtatttgga tgaaaatgct ttaaattgta atcttaaaat agaaagtggt ctgaaaactt
    26101 ctgtaagaga tgggtatatg aatttatcag gattagaaaa tcaggtaaat aagattataa
    26161 atgataatgg agataatact aatcaaaaag aatatatatc taaattagta ttagattcag
    26221 tagttagaaa tatgtttaaa tctctagtgt atttatgtga aacttataat gtaaatgaag
    26281 tagtatttgc tggtggagtt tctgcaagta agtatattct tagagaatta agcatgaagt
    26341 taagaaaaaa gcatatagaa gcttatttta cagaaccaca atattcaact gataatgctg
    26401 tcgggtgtgc tataatagga ttgaataact ttttaggaga aagagtatga aactaagagc
    26461 tttagatata agtgaagcaa actcatatat taaaagaatt ttaataaacg acccaatact
    26521 ttctaatctt aaagttaaag gagaaatttc taattttaag gttcatagta gtgggaatgt
    26581 atatttatca cttaaagatg aaacatcaaa gttaaactgt gtaattttta aaagtaattt
    26641 taatcgcaat ttaaaattag ataatggtgt aaaaattata gctaatggat atatatcagt
    26701 ctatgaaaga gatggtgcgt atcaattata cattaatgaa attgaaatag agggaatagg
    26761 aaatttgcac atagagttta atagattaaa ggaaaaactt aataaagaag gattatttga
    26821 ccctaaatac aagataccta tacctaaaat gccaaactca attggcgtta taacatctcc
    26881 aacaggtgct gttataagag atataataaa tgtaataaaa agaagatatc cgaaagttaa
    26941 tataaagctt tatccagtaa cggttcaagg agataaatca gctgaagaaa tctgtgaagc
    27001 aattagattt ttcaatcata tgaagaatgt tgatacactt atagttggta gaggtggggg
    27061 ttcaatagaa gaactttggt cttttaatga tgaaatggta gcaagagagg tttttaattc
    27121 tcaaattccg ataatatcag cagttgggca tgaaacggat tttactattt gtgactttgt
    27181 gtctgatatg agagccccaa cgccatcagc agcagctgag attgctactc catcattgga
    27241 tgatataaac tataaacttg gaaacataaa aagtaggatg agtaagtctc ttacaaatca
    27301 aatagagcta gaccaatata ggttagaaac agtatttaat aagataaata actatttaga
    27361 ttcgtacaca ataaaagata aagttataca attagacaaa atatatgata aaataatttt
    27421 tggaatagaa aataatttaa aattagaaga tgaaaaatta gtaaaaattg gagcattact
    27481 ccataattta agtccattag ctactatgga tagaggttat agtataactc aaaaaaatgg
    27541 aaaagtaata aatagtataa aaggattaaa aataaaggat agtatagata tagtattaaa
    27601 agatggaaat ttagaatgta tgatagataa aattgaaaat aaagagggat agtatgaatt
    27661 taacctacga agaagcttat aaaagattag aaagtatttt aagtgaatta gaatctaaaa
    27721 atgctagctt ggatgaatct ttaagcctat atgaagaagg tataagcctt tataaacatt
    27781 gtaataagct tttagatgat gctaaattga aaataagtaa atttaatcaa ttgggaatag
    27841 aagaagactt taaaatagag gaggaataaa attggaattt aaacaatgtc taaaagaaaa
    27901 agcaagtttt gtagaaaaag tacttaaaga atatatgcct aaagaagaag ggtatcaaaa
    27961 aactgttatt gaagctatga actatagctt aagtgctgga ggtaaaagat taagaccaat
    28021 acttacattg gaggcttgta aaatagttgg aggtaatgaa gatgaagcta ttccttttgc
    28081 tattgcaatt gaaatgatac atacatattc actaatacat gatgatttgc cagctcttga
    28141 taatgatgat ttaagaagag gaagaccaac aaatcataaa gtatatggag aagctatggg
    28201 gattttagca ggagatgctt tgttaaacta tgcttttgaa gtaatgcttg caggctcaat
    28261 caataaagaa aatccagaaa agtatctaaa agctataaat gaaattgcca aaggtgctgg
    28321 tatatatgga atgataggtg gtcaagtagt agatgtagaa agcgaaaata aacaaattga
    28381 aaaagaaaaa ttagattata tacatatgaa taaaactgct gcaatgatgg taggctgtat
    28441 gagagcagga gctactatag gtggagcaaa ttctgaacaa atggaagaaa taactaaata
    28501 tgcaaaaaat atcggattat cattccagat agtagatgat atactagaca tagtaggaga
    28561 tgaagctaag ttaggtaaaa aggttggaag tgatatagaa aatcataagt caacataccc
    28621 atctctttta ggtttagata aatctaaaga aattgcacat aatttgattg atgaagcaaa
    28681 gaaaagtata gaaaaattat ctgatgatgt agatttttta aagggattgg ctgaatatat
    28741 aatagataga gaatactaaa atatacagct aaaatcgagg agggaaaaat ggattttttt
    28801 tcggagattt ttaacaatgg ggctttggga ataagtctga ttgcttgttt cttagcacaa
    28861 tttataaaaa tatttactgg taaggaaaaa agaattgagt tatcaagaat acttatttca
    28921 ggaggtatgc ctagttccca tagttctttt gttacaagtt tagctacagt ggtaggtatt
    28981 gaaaaagggt ttaattctac tgattttgct ataattaccg tgcttgcctt aattataatg
    29041 tatgatgcag caggtgttag acgggctgtt ggtaaacaag ctactatatt aaatcaaatg
    29101 gtggcagata tacagcatgg aaagcatata gagcagaaga aattaaaaga attaataggt
    29161 catacaccac tagaagtttg gtttggagct ttacttggaa tagtaacagc tttaatattg
    29221 atgtaaattg aaaatttaca gaataaaggt gaggataata tgtataaata tttagataaa
    29281 gtaaattctc caaaggatat aaaaaatatg agtatagaag aaatggattt acttgcaaag
    29341 gatataagaa agttcttagt aaaatctgtt tcaaagacag gaggacactt agcttctaat
    29401 ctaggagtgg ttgagttaac attagcactt cataaagtat ttgatagccc taaagataag
    29461 attgtatggg atgtagggca tcaatcttat gttcataaaa tagttactgg aagaaaagat
    29521 tgttttgtat ctttgagaca gtttaatgga ctaagtggtt ttccaaagga gaatgaaagt
    29581 cctcatgata tttttgatac aggacatagc agtacatcta tttctatagc tacaggtatt
    29641 gcctgtgcta gggatataaa aaaagaaaac tatagtgtta tatctgtgat aggagatggt
    29701 tctataacag gaggtatggc acttgaagct ttaaatcaat taggatatat agataccaat
    29761 atgatagtaa tccttaatga taatgagatg tccatagata aaaatgttgg aggaatgtct
    29821 aaatatctgt caagtattat aagaaactca acagttgaga aaatgacaga tgaagttgat
    29881 aaaattttaa atgttacaca aacaggtgaa attttatcta agactgcaca tagatttaaa
    29941 gataaactaa tgtatagctt ttctcctcag gactgttcat tttttgattc attaggtata
    30001 agatattatg gacctataga tggccataat actaaagaac taatagatat attgagaaaa
    30061 gctaaacaca aaaaagggcc tgttctttta catgtaatta cgaaaaaagg aaaagggtac
    30121 agatttgcag aagaacaacc tgataaatat catggagtat caaagtttga tataaagaca
    30181 ggtgttacat ctgcaaaagt taagtctatg tcaattagtg ttggagaaaa acttgttgat
    30241 atggctaata gtaatgaaaa tatagtagca ataacagctg caatgccatc tggtacaggg
    30301 ttaaacctgt ttgaaagtgc atatccaaaa agatattatg atgttggtat agctgaacaa
    30361 catgcaacag gatttgcagc aggtcttgca aaaaatggta tgaaacctta ctttgctgtg
    30421 tactcatcat ttttgcaaag ggcatatgac caagttattc atgatgtatg cataacaaaa
    30481 aagccagtta cttttcttat agatagagct ggattagttg gaaatgatgg agaaactcat
    30541 catggtatgt ttgatttaag ttacttaaat tctattccaa atattgtagt aatggctcca
    30601 aaagacacaa gagagatgga gcttatgatg gatttgtcat taaaattaga ttgtccacta
    30661 gcaattagat acccaagagg aagtagttat tacctagata agggagagta tggagagatt
    30721 gtactgggta aatatgaggt attagatgac ggtcaagata cagtcatact ttgtattgga
    30781 agtatggtaa aacatgcttt agaggctaaa gaaatattat caagagaagg aataaatccg
    30841 acaatagtta atgctagatt cttaaagcca attgatgaag gtatgttaaa agcattatta
    30901 aaaaatcata aaaatgtagt tactatagag gataatattg taactggagg atttggaagt
    30961 agaataaata agtttattat agataatgaa tataacgtaa atatacttaa tatagcaata
    31021 ccagaagaat ttgtaaaaca tggcaatata gatgaattat atgactttgt aggattatcg
    31081 ccaaaaagta ttgctgataa gataaggaaa ttagttattg agtaaaaatc taatgtagat
    31141 attgtaaatt aaaatttata taaaatatag aatttatcaa ttagaataaa tatcaagtgt
    31201 gttacttcaa gctactttag ttatcattaa agtagctttt aacagaaagg taattgtatg
    31261 aaaaaaagaa tagatttatt attagtagaa caagggtact ttgaaagtag agaaagagcc
    31321 aaaaaagcaa taatggctgg attggttttt gttgataatc aaaggtgtga taaggctggt
    31381 acagaagtaa aagaagattg tagtatagaa gtaaaaggaa atccaatacc atatgtcagt
    31441 agaggtggtt taaagcttga aaaagcaatg aaaaactttg atttgactat tgatggaaag
    31501 gtatgtatgg atataggggc atccacaggt ggttttacag attgtatgct caaaaatgga
    31561 gcaataaaag tattttctat agatgttggt tatggtcaac ttgcatggaa attgagacaa
    31621 gatgataggg ttgtgtgtat ggaaagaacc aatataagaa atgtgacaat agaagataca
    31681 aagcagtttg ctgattttgc gtcaattgat gtgtctttta tatcgttaaa gttagtactt
    31741 ccaaaggcaa aggagttagt acgtcatgat ggggaagttg tcgctttaat taaacctcaa
    31801 tttgaagcag gaagagaaaa agtaggtaaa aaaggagtag ttagagaaaa atctacacat
    31861 atagaagtta taaaaatgat ttctgatttc tcagtcgaaa atggatttga gattttaggg
    31921 ttagattttt ctcctattaa aggtcctgaa ggtaatatag aatatctaat tcatttgaga
    31981 aatggaaatg aaggatatga atttgatggt gagacataca ataacaaaat agtagaagtt
    32041 gttgaagctt ctcataattt agacaaatag gttaaaaggg ggcttagatt tgatcctcga
    32101 attatatatg aaaaattgtg ccttagttga agaactaagg ttgaatattg ataaaaactt
    32161 aaatatactt acaggtgaaa ctggttcagg aaaatctata ataatagatg cacttggact
    32221 ttgtttaggt gataaatatg ataggtcttt tttaagaaaa ggaacagata agggacttgt
    32281 tgaggctgta tttttttcag acaatagata tcttaaaaaa atattagaag aaaatgatat
    32341 aagtatggaa gatgataatt tattggtaat aactaggttg atatactcag atggtaagag
    32401 tactgctaga gtaaatggaa ggactgttaa agtgtctctt cttaaagaaa ttgcatctac
    32461 acttatagat atacatggtc aacatcaaaa tcaagctttg tttaataaag atactcattt
    32521 aaaatttcta gacctttttg gagaaaatga attggaagag tttaaaattg cttataaaaa
    32581 tgtgtaccat aagtatagtg aagtaaaaaa agctttaaat tgcttgacag aaaataaaga
    32641 tgagatgcaa attcaacgag aaatagattt attaagattt caaataaatg aaattgaagc
    32701 tgcaaatcta aataagaatg aatatgaaga tttattaaaa caaagagaag tttatagaaa
    32761 tagtgaaaag atatataata atctaaattc gagttatagc aagttacata atggtgagta
    32821 taatgttatt gacctaatag gtcttgcatc taaggaactt aatgacatat ctaaatatga
    32881 cagtgtttta agtgaatata gtgatactgt agaaagaatc atgtatgagt tacaagacat
    32941 atctggtgag ataagaaatt acaaagataa tatagacttt gaaccttatg agctagagca
    33001 gattgagctt agaatcgacg agattaataa cttaagaaga aaatatggag acagtataga
    33061 tgatatattt gagtattatg gaaaaattaa agatagatta gatgaaatat taaacagaga
    33121 tgaaagagtt gaacagttaa gaagcaaact gatgaatata gaagaagatt taaaaataaa
    33181 agccagtaaa ttgactaaag caagaataga agtagcaact actttagaaa aaatacttct
    33241 tgatgaatta aaaagcttaa atatgaaaaa tgtaatgttc aaggttaatt ttgaaaaagg
    33301 tccatttact ttagatggag tagatgatat tgaatttatg atatctttta acttaggaga
    33361 agatattaag cctatatata aagttgcatc aggtggtgag atgtccagat ttatgttagc
    33421 gtttaaaacc atattggctg atatagatga tattgataca ttagtgtttg acgagataga
    33481 tactggaata agtgggatag cagcacagat agttggtgag aaattgagtg atatagcaaa
    33541 gaaaaaacag ataatatgta taactcattt acctcaaatt gctgcaaatg cagacactca
    33601 ttattgtata gagaaagata cttcaaataa taggacgttt accaatgtta gtaagttaaa
    33661 tgaatctcaa cgaaaaaatg aaattgccag acttatagca ggtaacaata taacagaaaa
    33721 aactattgaa catgcaagtg aaattattga gttggctaaa aaatgttagt gaaaaacaag
    33781 cattttaata ggtgtaaaga atttttatag aaatgcttta catctatttt ttatgatgaa
    33841 attattttaa aatatctagt gcctatattg aagaaaagta ggtttgaaaa tggaatgaac
    33901 tttgtataaa tttgtaactt tgaaatttct aaatgcttaa gtataatatt atttattaag
    33961 gtagtagtga ggggaggcat aaaatgaata aaaaagatac tatgcactta atttttataa
    34021 ttgtaatttt tattttatta tggtatattg tggtagcagt aagtgagatt aataaaaata
    34081 ttgaaagttg tagtaacaaa ttagattatt tgtcacaaga gcttatgagt actaagtcgg
    34141 atattagtaa tactataaat gaagaaatgt caaaaagcta tataacaaaa agtattgact
    34201 taaaagtgaa aaaaatcgag aaaaaagaat gtattattga tatagatgtt caattaagta
    34261 aacttggaaa aaatggaaaa gtatttttta tgtataaaga agatagtgat aaatggaatg
    34321 aaactgagat gacaaaacat ggagaattat catatgcatg tgaaattaag ataaatacag
    34381 gaagtgaata tgactataaa gtagttacaa gtggagctat atctgaaagt tctgatgtgc
    34441 aaaaattgac taaatctgat tatatgcctg aacaacccat atttaattct gggataagag
    34501 ataatagaga atactttatt gaaatagtag aaaatagtaa tcttttcaat catgagagtg
    34561 aaaagtcaaa aaataaagtt tatgataata ttagattaga aaaagtagat attattgtaa
    34621 atgaaggaaa aaaagacact atatacaaag ccaaattagc tgaaggctta gatgatggca
    34681 aaactaataa ttcaaataga aatcaagtga attatgaagt aagttttcct aaaagggata
    34741 ttgatgacat aatttatatt aaagctaaac ttacatacaa taatggagta gaatatacaa
    34801 aggatataac tgaagatata actatgggat tacaagattt agaaaaataa atacatattt
    34861 aaataaatat tcaaatagat ttgttcaata aaatagagta taaataaaac tgtagtagtt
    34921 ttacttatac tctattaaaa aattataatt ttaattccat atatctagcc ccttcaattg
    34981 gattataagt ataaggttct atatcataaa aaccaaactt ttcgtataat ccttgtgcac
    35041 ttttcatgct tggaagagta tctaatctca tgtgtgtata tccaaatttt ttagcttctt
    35101 caatgatttc ttccaatagt atttttccta tttttaatcc tctaaattga tttcttacat
    35161 ataatctttt taattcacat attttacctt caagtttttt taaagcaaca caacctgcca
    35221 aattttcatc aacaaatgcc aatatcaatg agcctgaagg ttttttatat tttcctggaa
    35281 gtgtttttaa ttcattatta aagtcttgaa aacataagtc tatatttaga gaatttgaat
    35341 attcagtaaa tagaagtttt acgttttcaa ggttttctaa atctttttct tcaacaaatt
    35401 gtaaagttat attttgagcc attttatccg ccattatata cacatgttta tataatgact
    35461 tcacctcctt gatatatgta tatttttctt aatatcaatt agattttaat accattactt
    35521 tttcaccacc atagtcaagc tctccattaa actcaaatcc aaaatcttta taaagcttaa
    35581 tagccatttt attgtcttcg aatatactaa gatatagttc atcacagtta tattcatttt
    35641 taagatgatt tacaagaaaa tctaatgatg cttttccata tcctttacct tgatattttg
    35701 aatcaatcat aaatctatca agccaaactc tatcatcttc taaacgacca tacatagcaa
    35761 atcctattaa agaattttca tcatatattc caacaggtac ccaagctgtt ataaatttag
    35821 attcagcaat agaaagtgca ttgctttcta tataatctaa ctgactttta tctacagaca
    35881 aactaactaa atcatgccaa ttatttatgt ctgcaattct tacatttaat ttcaagatat
    35941 taccgccctt caaacttttt gttacaatct aatactatca tattatgtat taaaaatcac
    36001 ttattaagat tttaaataaa ctttttaaat gaataaattc tcaggaaaaa ggaattataa
    36061 atacataata atacagcata ggagtagatg gatatatgaa caaaataaat aaaaatttca
    36121 aaaatatttg gataatatta ggagctgtat cgatttttac catggttata atttttaata
    36181 aaaattgtaa tgttaataaa gagactcaaa tagaagcttt tacaattaag aatcatgata
    36241 aaaaatttgt atatcctatg ggaaatataa ttggagtaaa agcaaataca gatggtgtat
    36301 tggtcttagg atatgaggaa gaggatgtag actatattgg aggcatacaa atcggtgata
    36361 atatagtaaa aatagataat aaaaggataa aaaatagtca agatgtatca gagattttga
    36421 ataagattaa aaaaagtaaa gttgaagtga cttttgagag aaaaaataag tataaaacag
    36481 aagtaataga gactaaaaaa gaaaatggca agtataaact tggattatgg gtaagagata
    36541 aaatatctgg aattggaact atgacttttt acgaccctag tatggaaaag ttcaaagcaa
    36601 ttggtcatgc cataaaagat tcggatacta atgaactatt aaagataaag caaggatata
    36661 tatataagcc agaacaatta aaaatagtaa aagctagtaa tgaaaaagta ggtaaaataa
    36721 aaggagattt taatgacagc aatttaatgg gtaatttctc aaataattca gagttaggta
    36781 taagtggtaa tattacagaa aaccataata aagagtttaa tgtagcaaat aaagaaaagc
    36841 aattaataga agttggaaga ccgcaagatg taaaaatagg aggtgcagta attctttttg
    36901 aggataaaaa caaaaatata acaagctatg atataaaaat agaaagtata gtatatgata
    36961 aaggaaatta tagagatatg gtaataaaag tagtagatga caagttattg gaatacacag
    37021 gaggtatcgt acaggggatg agtggagctc caataataca aaataataaa attattggtg
    37081 caataactca tgtttttaga gataatccga aaaaaggata tggtattttt atagatgaaa
    37141 tgataaaatt gtaggtgagg cattaaaaaa ttttattatt ttatcaatta tctaggagga
    37201 atataatttt ggagtgtcga atatgcttta gagtagataa ttaggaagca attgtgtaaa
    37261 aagtttagtt ttctgtaata agaagatgtt ttttaatggg gggattttta gtggaaaaaa
    37321 tcaaaatagt tttagcagat gacaataagg atttttgtca ggtattaaaa gagtatttgt
    37381 ctaatgaaga tgatatcgat atattaggca tagctaagga tggaattgaa gcattagact
    37441 tagtaaaaaa gacacaaccg gacttattaa tactagatgt aataatgcca catctagatg
    37501 gattaggtgt aattgaaaaa ttgaatacta tggatatacc taaaatgcca aaaataatag
    37561 tactatcagc agtaggtcaa gataagataa ctcaaagcgc aataaatcta ggagcagatt
    37621 actacatagt aaagccattt gattttgttg tgtttataaa cagaattaga gagctagttt
    37681 ctaatagagt tacacaagtt gagccaaaac ctagaccagt tcaagagact caaatgacta
    37741 gaagtgattt tgttaagaat gtaggaaata tagagacaga aatcacaaat ataatacatg
    37801 aaataggagt accagctcat ataaaaggat atttatattt aagagaagca ataaaaatgg
    37861 ttattgataa tgttgagctt ttaggtgcag taacaaaaga gttatatcca agtatagcta
    37921 aaaaattcaa tacaacacca agtagagttg aaagagcaat aagacatgca atagaggttg
    37981 catggagtag aggaaaagtt gacacaataa atcaattatt tggatatacg gtacacaata
    38041 ctaaaggaaa accaactaat tcagaattta tagcaatgat tgctgataaa ttaagactag
    38101 aacatagtat ggttaaataa acaagacata aaaagtaagg ctttttaatt aaggcattgg
    38161 ctataaatgc gtattacaag cagcgaaacg gtataaccac tagggttata ccgtttcgct
    38221 attttaataa atataaaaat tttatttatt atttgcttac tatatcaata taataatttt
    38281 attatactat ggatatagta tgtgtcttta caagttgtaa actgacagtg gtttattttt
    38341 taatataaat attgactttg atgcaggtaa actttgtatt tttaagcgta ttatggaata
    38401 tgttaaataa aaaaatgatg aaatatagta ttgtaaatgc caaagatgca aaacaaactt
    38461 aaaacattta ttttattgtt aagtaatgct ataatataat gtgattttaa taatgatagt
    38521 ggaggtttaa atatgagagt cgaggcccct ataaaagtag atcgaaaaac caaaaaactt
    38581 gctaaaagag ttgaaagtgg ggaaatagca gttataaatc atatagacat agatgaagtt
    38641 gctgcaaact ctttagtaga agctaaaata aaacttgtca taaatgcggc tccttctata
    38701 agtggtaggt atcccaataa aggtccaggg atattaactg aaaataatat actaataatt
    38761 gataatgttg gagaagagtt atttgaagaa ttaaaagaag gagaaactat agaagttgta
    38821 gatggaaaga tatatagaaa aggaaaattt ttaggtgcag gagaagtttt agacaagtat
    38881 gaggtttcac accaaataaa ggctgcctat gagaatttag ctgtagaatt ggatagattt
    38941 atagataata ctatagatta tgctaaaaaa gaaaaaggat ttatacttgg agaagtagaa
    39001 attccaaaag tgaaaactaa ttatgctaat aaacatgtat tgatagttgt aagaggtcag
    39061 gactacaaag aagatttaag tactatgctt tcttatatag aagaggtaaa gccagtattg
    39121 gttggtgttg atggaggagc agacgcttta attgagtttg gatatactcc agatgtgatt
    39181 gtaggtgata tggatagtgt aagtgatgaa gctttaaaaa aggctagtga aatagtagta
    39241 catgcttata cagatggaag agcaccaggt cttaaaaggg ttgaggaatt aggcttagat
    39301 gcagttgtat ttcctgcacc aggaactagt gaagatatag ctatgcttat cgcttatgag
    39361 tataaggctg agttaatagt agcagttggt acacattcaa atatgattga ttttcttgaa
    39421 aaaggaagaa aaggtatggc aagtactttt ttagttagac taaaaatagg ttcaaagctt
    39481 atagatgcta aaggcgttaa tttgttatac agaagtaaat taaaaataaa gtacatatgg
    39541 gcattaatag caacagcatt atttcctgtg ttggtagttg catcattgtc accaggagtt
    39601 caacagttca tacaactaat gcaattaaag tttagagtat tattacagat gtaaatttta
    39661 ggaggataaa tatgcatatt aatatgaaat attatatagt cactataggg gctatattta
    39721 tagctttggg tataggcatg ttagtaggat ttaatttaaa taataatcaa caattaagtg
    39781 aacaacaagc aaacataata aatgatttgg atgataagtt tgatatctta aaagaaaaaa
    39841 atgataaact tgacagtgac ttagcaagtg taaatagaga ttatgaagag gcagttaatt
    39901 ttataaataa aaatgtagat aagattttag ctggttcgct gaatggtaaa agtataggaa
    39961 taatatctgc taatgaaaat gacgattata caaaaaatat agaagacata ataaataaat
    40021 ctaatggaag cattgcattt aatataatat taaaagaaaa cataacaaat ccagaaaagt
    40081 taaaggaaat gtcaactaaa ctaggtacag atgtaaaaaa tgcaaatgat gcagttaatt
    40141 atataattga cactcttaaa aaggaagatg caagtgacat tttaacatat cttcaagaac
    40201 tagatgtaat aaagtttaat tttattggag atacatatct aaaatatgat tccgttgtaa
    40261 tagctggtgg aaatgatgca aaagacagta caaaacagtt tgaaaaaata gaaaaatttg
    40321 tagtttcaaa acttaaatca gagaataaat atttagtaga agttcaaaat acaggagtta
    40381 aaacttctta tgtagaacta tattctaaaa acaaagttgc aactatagac aatattgatg
    40441 aaggtatagg aagtatatct ctagctattc ttctacaaca aggtaatata gttggtaatt
    40501 ttggtagatt ggatactgca acttcattgt tgccatctat taaatagaaa taattttaag
    40561 gagagaaaaa atggattctt atataagtat aataatacca gcttataatg aagaaagtag
    40621 aataaaatat acattagaaa gcatattgga cattaagcaa ataaatgaaa taatagttgt
    40681 ggatgatggt tcaattgaca atactgctaa ggttgtatct gagctagaaa atgaaaagat
    40741 taaatttttt aagttagata aaaatagagg taaaggatat gctttaaatt atggtatcaa
    40801 attagcaatg aaaaatgcag atattatagg ttttctggat ggagatttag gaagttctgc
    40861 taaagaagtt gaaaaattaa tagtacctat attaaatggt gaggcggatg ttacaatagc
    40921 caagtttcct cctgcaaaga gaaaaggagg tttgggattt gtaaaaggtt tagctaaaaa
    40981 ttctgtgttt gagatgactg gtgttgagtt agatgcaact ttatcaggtc aaagaatatt
    41041 taaaagagaa gttttagaaa agtttgatga aatacctttt ggatatggag tagaagttgg
    41101 aatgaccatt gatatattaa agtatggatt taaaattaaa gaagtattag taaatatgac
    41161 tcataatgaa acaggaagaa atttaaaagg ttttattcat agagggaaac agtattatca
    41221 cataaaaaag gttttaggac aaaaaaagaa agaatggagg taatatctta gtgaaagatt
    41281 atatacttta taccatttta ttattaactg gattatttgg aacctatgct gtaattccac
    41341 tatttaaaaa tttattgata aatggaaatg tacttagacc aaactataaa aaagatatga
    41401 taccagtaag tatgggaatt gtatttttgc ctatgattgt aatcaatgga ataatacttg
    41461 ctttctttac tacagaattt aaggatttag cgtatatatt tatgtttatg tttggtatga
    41521 tatccatgtt ttttgcaggg atactagatg atgtcattgg aaatagagat gtaagtggtt
    41581 taaaggggca ttttaaaagt ttatttaaag gaagtcttac aacaggaggt tttaaagctt
    41641 tatttggtgg atttgtaggg cttatagttt ctgttgctat atcaaaagac ataattgata
    41701 taatagtaaa tacactaata atagctctat caactaattt aatgaatctt ttggatttaa
    41761 gaccaggaag ggcaataaaa gcatatttat tcataatggt aattatattt ttaacattga
    41821 caggatttgt tcaagttctt ccattactta tagtaccaaa tgtacttgca tactttaatt
    41881 atgatttaaa agcaagggct atgatgggag atactggttc aaacgtcctt ggaatatcta
    41941 ttggaatgct catttctttt gggtatccat ttaacattag attaggatgg ttaatatttg
    42001 tagttattat gcatatattc actgagaaat actcattaac taagataata gaaaaaaata
    42061 aactattaaa ttttatagat aaattgggaa ggtagtatta tgataagcaa aagagtgggg
    42121 attgttgaat caatagtaag tcaaactgaa acactagatg atataagagt aaatataaat
    42181 ggagaaatac aaagggcata taattatccc aaaatatctg gaactataaa tataggtgac
    42241 gaagtggttt taaatacaac agcagtagaa ctgagcttag gtacaggtgg ataccatttt
    42301 gtaataacta atttaaataa tatagaatct actttaactg aaggtggaca tataatgaaa
    42361 cttagatata cgcctcttca aataaaagtg gattctgttg aggaacaaga aagtatttat
    42421 catgataaat ttgcgaattt taaaggatta gaaggtcttt ctgttgtagt agggactctt
    42481 catagtatgt taactccatt tgcagcttca tttaaaagaa ataatccaaa taaaaaatta
    42541 gtgtatataa tgactgatgg agcatctctt ccaatttatt taagtaaaaa tgtggatact
    42601 ttaaaagaaa aaaaattaat agatagtaca ataactatag gaaatgcgtt tggtggagat
    42661 tatgaatgta taaatatata tactgcatta attacagcaa aagaaatact aaaagcggat
    42721 gcagtatttg tcagtatggg acctggaatt gctgggacag gaacaaaata tggatttaca
    42781 gggattgaac aaggctctat tcttgatgct gtaaaaaaac ttgaaggaag agcaattgca
    42841 attccaagaa ttagttttgc tgacaaacga gaaagacatc aaggtatttc acatcatagt
    42901 atgactgtat ttaaagaaat tgtaaatgta aatgtagaca taccaataac tatatatgat
    42961 gatgaaaaat tgaactttat aaaagagcaa atcaatttaa atggtttaga caaaaagcat
    43021 aatataattt atatagataa taataaaacg aaagaggatt tagactattt taatctaaaa
    43081 gtaaagagta tgggaagaaa ttatgaacaa gatgaggcat ttttcaaagc tgcaagcaca
    43141 gcagcatatt acttaatgga ggtttaagat ggtattagaa gaaaaaacta ttagtagtga
    43201 tagggtttat acaggtaaag taattacttt aaaggttgat actgttgaaa ttccagggca
    43261 aggatatcaa aaaagggagt tagtagaagt tggtggagca gtaggaatag ttgcaatcac
    43321 agatgataat aaagttgtgt tagttaagca atttagaaaa cctatagaaa agccaatctt
    43381 tgaaattcca gctggtaaac tagaaaaaaa tgaaagtcca aaagaatgtg ctgaaagaga
    43441 attaaaagaa gaaacagggt atagtgcaaa aaatattaag ctaatacata aatttttcac
    43501 ttcagcaggt ttttctaatg agataatgtt tgtatattta gcaacaggtc ttactccagg
    43561 agagaataat ttagatgcag atgagttttt agatgtctat gaaattgagc ttgaagaagc
    43621 atataacatg gtattaaaaa atgacgttga agatgcaaaa acatctattg gattattatt
    43681 agtaaaagat atgtttaaaa attaatattt ataaaatgaa taaccctttg tcctaaagca
    43741 tattatttta taaaggacaa gggggaatta aattgagaag aacatataga aaaaatgatt
    43801 ttagagaatt aaatagacaa ataattatta taggattgtt gtttatgatg tctattgttg
    43861 ttgggtctta cataaataaa atattgccag gttctagtaa caatatatta aacaatataa
    43921 atcctgctgt agagtattat aatctaaata taagcataaa agatacagtg atacaaaatt
    43981 taaaatcaga tgcaatcttt atgggctcaa tagctctatt aagtctattt gtagtaacaa
    44041 ttccagcagt gttggtagct tttgtattaa aagggatgtc aattggttat acaataaata
    44101 gttgtatatt ggcattaaaa tttaaaagta taaaaatgat tttaataata ttatttaaaa
    44161 atttgattat tattccatgt gcaatcatat tagcattaat tagtttaagt tattttaaag
    44221 aaatggttta tgaatttaaa aagaaaaata gaaaaaatat gcaattttta attaagaggt
    44281 atatactaaa tattataatt ataatagcac tatcattggg gttgcaatta atattaaata
    44341 ctgcaagtat tggtattatt aagttcctag ctaaataaag gagtttataa tggatattat
    44401 agagggatat atagattaca taaaagataa aaaaaaatta tcagagaata cagtggcatc
    44461 atactttatg gatataaaaa aatatatgga atatataaat caaaaaagca ttaaactagt
    44521 agatatagta gaaaatgata ttataagtta cttaataaag ttggaaaaag ataatgtatc
    44581 aatagctaca atagcgagga tgatatcctc tataaaatct tttcatgatt atttattttt
    44641 aaatcatata tgtacaaata atcctgcaaa ggatataaaa aaacctaaaa taaaaaaaga
    44701 aaatataaat atacttacag aagaagaaat tgaaagacta ttaaattttc caaagttaac
    44761 aacacctaaa ttgattaggg acaaggctat ttttgaagta ttatatggta ctggtataaa
    44821 agtatctgaa ttagtagaga tgaatataga ggatattgac ctagatattg attatatata
    44881 ttgtaattca tgttgtaaaa atcaaagagt gataccactt tgtgatatta caaaacttta
    44941 cttagaaaag tatctaaaag aagcaagacc taaaatggct gtagaaggtg aaaaatcatt
    45001 attcgtaagt tcattaggtc aaaggtttac acgacaaggt ctttggaaag tcataaaaaa
    45061 atattctaat ctagccaata tagataaaaa tataaatcct acaatgctta gacattcatt
    45121 tgctattcat ttgttaaatg aaggtgctaa cattgctgtt gtaagtaaaa ttttaggaaa
    45181 tgttaattta tctagtttgc aagtatattt aaatcatata gataaaaacg tgagaagaga
    45241 aataaaggag aaacatccta gaaatgatgt tgatgtagaa ttacaaaaag cagaagctaa
    45301 atagactata tagcaaattt tataatttag aaggagcata tttatatgag cagagtaatt
    45361 tggattgtaa ttgatagtgt tgggatagga gcattgcctg atgctgaaaa atttggtgat
    45421 agtaaggatg taagtaccct tggaaatata tttaaggaat atccagatat tcagattcct
    45481 aatatgagaa atttaggtat aggaaacata gatggtatag atttttttga aagtataaaa
    45541 gaaccaatag gttgttttgg aaagtgtaag gaaatgtctc agggaaaaga tactactaca
    45601 ggtcattggg aaatgactgg tataatagtt gacaaaccat ttaagacatt tgaacatgga
    45661 ttctctaagg agataataga agagtttgag aaaaaaactg gaagaaaagt agtaggaaat
    45721 aaaccagctt caggaactgt aataatagat gagtatggag aacatcaaat aaaaactgga
    45781 gatgtaatag tatatacatc agctgatagt gtatttcaga ttgctgctaa tgaagaagta
    45841 attcccctag aagaactcta taatatgtgc aaaatcgcta gggaaataat gatgggggat
    45901 aatgcagtag ctagagtaat tgctagacca ttcataggta agaaaaaagg tgagtttgtg
    45961 aggacttcaa atagaagaga ttattcacta gacccatttg aaccaacagt tcttgataat
    46021 ataaaagaat caggacttga tgttttagca gttggaaaaa tagaagatat ttttaatgga
    46081 aaaggaatta cagatgctat ccacactaaa agcaatatgg atggagtgga tgaaacttta
    46141 aattatatga aacaagataa taaaggtcta atatattcaa atctcgttga ttttgattct
    46201 aaatatggtc ataggagaga cccagaagga tataagaaag ctcttgaaga gtttgatagt
    46261 agacttcctg aaataatggc taatatgaga gaagatgata ttttaataat caatgcagac
    46321 catggaaatg acccaacata taaaggtaca gaccatacaa gagaatatat acctgttatg
    46381 atttatggca ataagattaa aaaaggtttt aatttaggag taaaagatac ttttgctgat
    46441 attggagcaa ctgttgcaga tatactaaat gtaaagttgc ctaaacatgg gtcaagcttt
    46501 aaagaagatt tattttaaac tataaaatga taaaaactta gcttaatagg atggagagat
    46561 tggaatgttt gaaaagatag cacaaagtag taaatttata aattctaaaa gtgatataaa
    46621 acctaagatt ggtttgatat taggttctgg actaggtgat ttagcaaatg atatagaaga
    46681 ccctgtcgca attaaatata gtgaaattcc aaacttccct gtatcaacag tagcaggaca
    46741 tgctggacaa cttgtaattg gaaaacttga aggaaaagaa gttattgcta tgcaaggtag
    46801 atttcattac tatgaaggat attctcagaa agaagctact tttccagtaa gggttatgaa
    46861 agaattagga gtcgagattc ttatagtaac taacgctgct ggaggcgtaa ataaagaatt
    46921 taaagcagga gatttgatgc ttataagaga ccatataaac tttagtggaa gtaatccatt
    46981 agtaggaaaa aatgatgaca gatttggtgc tagatttcca gatatgtcag atgcatattc
    47041 ttctaaatat gtagatgtag ttaaaaactg cgctaaagaa tgcaatatag atgtcaaaga
    47101 aggcgtttat atgtttttta gtggtccaaa ctatgaaaca ccagcagaag tgagaatggc
    47161 acaaattttg ggagctgatg ctgtaggtat gtctactgtt ccagaagtca tagtagcttc
    47221 tcactctaat ataggagtta ttggtatatc ttgtataaca aatatggctg ctggcatctt
    47281 aaatcaacca ctaagtcatg aggaagtaat agaaactact caaaaagtta aatcagaatt
    47341 tatgaattta gttaaaacaa cggttaaaaa tttataagtt aaagcttatt atagattttt
    47401 tgaggaagtt tataataaaa tagttaatgt ttaatttgaa aagaattaat aaaaagctgt
    47461 cttgatatag aaagttattt ctaataaagg acagtttttt tataatacta caacatttga
    47521 aagtgtttta aagtttaata tttatttaaa ttattatagg ttagaatgga gatttcaatt
    47581 atgagaatgt atgatattat aaaaaaaaag agagataacc tagagcttag taaaaaagag
    47641 ataaactact ttatagaaaa ctattcaaag ggagatatac ctgactatca agcttctgca
    47701 ctattaatgt cgatttattt aaataagatg aataaacagg aaactgtaca tcttacagaa
    47761 gcaatgatga actctggaga catgataaac ctatctgaga tagatggaat aaaggtagat
    47821 aagcatagta ctggtggtgt tggagataag actaccatag ctcttatacc tttggttgct
    47881 tcatgtggag caccagttgc aaagatgtca ggtagaggtc ttggtcatac tggtggtact
    47941 attgataaat tggagtctat accaggtttt tcgacagaaa tggagataag taaatttatt
    48001 gattctgtaa ataagtctaa aatagcagtt tgtggtcaaa gtgcaaaggt agcaattgca
    48061 gataagaaaa tatatgctct tagagatgtt acagcaactg ttgataatat atcattgata
    48121 tcatcaagtg taatgtctaa gaagttagct tcaggagctg atgcaattgt gctagatgta
    48181 aaaactggtg atggagcatt tatgaagaca ttagaagagt cttttgagtt agctaaatct
    48241 atggttgata taggtactgg tatgaataga gatacaatag ggattgtaac agatatggat
    48301 gaacctttag gttttgcagt gggaaactct ttagaagtta tagaagcaat tgagacatta
    48361 aaaggaaatg gacctaaaga ccttgttgag ctatgcgaag tattaggagc atatatgttg
    48421 gttttatcaa aggtagcaca tgattttgaa gatggtgttg aaaaaataag agaatcaata
    48481 aaatctggta ctgctataca aaaactcaaa gagtttatag agaatcaagg tggtaataaa
    48541 gatgtagtag atgattattc tttattcaaa aaatcaaagt ataaatatcc agttaaatca
    48601 actaaatcag gttatataag caaaattaaa gctgaagatg ttggtgtatc ggctatgatt
    48661 cttggagcag gtagagaaac taaagatgat attttagatt tatcagcagg gataatttta
    48721 gaaaagaaag taggagatta tgtaaatgaa ggtgaagttt tagcttacat gtattataat
    48781 gatgaacaaa agctttcatt ggcaaaggga agatttattg attcctactc tattgtagat
    48841 tcaaaaatag aaaaaaataa attaatatat ggtattgtaa ctaaagaaga gattaaaaag
    48901 ttttagaaaa ttttagaaaa gtgattataa aaggcctaac atgtcaaaaa taatgttagg
    48961 ttttttattt tttgatagac tataaattat accaaaaata tatattttaa aacatttttt
    49021 acaatataaa taagtttatt ctacaagatg attgaaaatt aaagtatcta gttttataat
    49081 agaaaatgga taaatataca taaggagtag attatattat gaactttaaa gaaaatagat
    49141 tgaaaatagc agtattaata attgttattt tgattatctt agcaggtata tttgtgttta
    49201 tacaaatagg tccttatgat aaaaataaca aaaaagatgt aattatcgac gttccaagtg
    49261 gagcatctgt tggaaaaata tctgacatat tatatgaaaa taaattaata aagaatgaac
    49321 tattatttaa acttttggta aaggttagca ataaagctcc gtctataaaa tcaggaacat
    49381 acttacttaa tcaatcgtat tcaaacaatg atataataag tcttttagtt tcaggtaaaa
    49441 tatatcagga tggtataaaa gtgactattc cagagggagc tacatctaaa gaaattatag
    49501 caatgttagt aagtaaaaat ttaggagata aagctacttt tgaaaattta ataaaaaaac
    49561 ctcaagaatt ttatgataaa tttccatatt tgaaggaaga tggaataacc tctttagaag
    49621 ggttcttgta tccagaaact tattatttta attcaaagaa acaatcagaa gaagatattt
    49681 taagcgaaat gttaaaagta tttgacagta aatacacgga caagtttaaa aagaaacaaa
    49741 aagaattaaa tatgactctt caagaagtaa tggaaatggc atcaataatt gagaaagaag
    49801 ctgttcttga caaagacaga cctataatag caagtgtgtt ttataataga cttaaggtag
    49861 gtatgcctct tcaatcagat gctacaatac aatatatatt tgaagagaga aagaaaattg
    49921 taacatatga tgatttaaaa attgattcac catataatag ttataaaaat aagggattac
    49981 ctcctacacc aatatcaaat cctggtataa aatctattga agcagcatta tatccagaga
    50041 aaacagacta tttatatttt gtagctaaga ttgatggtgg aaataattat agtacaaact
    50101 atcaagacca tttaaaatac gttaaagaat ataaagaagc aagggataaa caatcaaaag
    50161 atacaaaagc aacaaataaa gaaaatacaa aaaaataaac catgattata aaaagtctaa
    50221 cagggtaaga ataatgttgg gcttttttat tgaaaattgt tattcttaga tttataataa
    50281 agaaagtgta aatatatata acaatatgaa tttaaaaaat aataaattaa tataaaactt
    50341 gaatatttat tattttaggg agtgttttta tgagtaatat agttaatgat ttggttgaag
    50401 attatataag agtgacatta aaagaaaaag aaggattttt aaaagattta gaaatttatg
    50461 cagaggaaaa tagtgttcct ataatacata aagaagtttc agatttatta aaagtcttgt
    50521 taaaggttca aaaaccaaag agaattttag aagtaggatg tgcaattggt tattcttcta
    50581 ttttttttgc aactattata ggaaaagatg cagatataat aactgttgag agaaatgaaa
    50641 aaatgataga aaaagcaaaa gaaaacataa aattagctgg atttgacaat aacataacta
    50701 tactagaagg agatgcagaa gaaaaactta gtcaagtcca aggtgaattt gaccttatat
    50761 ttatagatgc agccaaaggt caatataagc tattttttga tttagttata gataaattaa
    50821 aagatggggg acttttagta tctgataata ttttgtataa aggtatggtt gctcatgatg
    50881 attttgttgt aagaagaaaa aagactatag ttaaaagaat gagaaattat ttagattata
    50941 tatgtaattg tgactattta agcacatcat tggtaccaat aggtgatggt gtagcactaa
    51001 gttataaaga aagtaaaaga ggtgatgtag atggaattaa ataaagtaga acttttagct
    51061 ccagctggtg atttggagag attgaaaata gctataacat atggagcaga tgccgtttat
    51121 ataggtggag aaatttttgg aatgaggtct gcagcgaaga attttagtaa ggaagatatg
    51181 gcagagggag tagcttttgc acatgaaaga ggcaagaaag tattcgtaac agtaaatata
    51241 atacctcata atgaagattt tctacaatta gaagactatt tgttggaact agaagagata
    51301 ggaatagatg cagttataat agctgaccca ggagtgctta gtgtcattaa aaaagtaatt
    51361 ccgaatatgg aaatacattt aagtacacaa gcaaatacaa ccaattatct aagtgcaaac
    51421 ttctggtacg agcatggaat aaaaagagtt gtagtggcaa gagaattatc ttttgatgaa
    51481 atatctgaga taagagcaaa aacaccttta gatatggata tagaagcgtt tatgcatggt
    51541 gctatgtgta tttcttattc tggcagatgt ttaataagca actatatgac tggaagagat
    51601 gcaaacaaag gttcttgtgc tcaatcttgt agatggcaat atcatttggt ggaagaaaaa
    51661 agacctggtg agtattttcc aatatacgaa gatgaaaggg gtacattctt ctttaactca
    51721 aaagatttat gtatgataga gtatatacca gagttaatca aatctggtat aacaagttta
    51781 aaaatagaag gtagaatgaa gactgcatac tatgtagcta ctgttgtaag agcatacaga
    51841 atggcaatag atgaatttta cagagaccca gaaaactgga agttcaatcc aatgtggatg
    51901 gaagaactaa aaaaaggtag tcatagacat tttacatcag gtttttactt gaataaacca
    51961 acaacagaag accaaaatta tcaatcagca tcatatgtta gaaactatga ttttataggt
    52021 atagttagag aaacagaaga tgaagatggt cttattgttg ttgagcaaag aaataaaatg
    52081 tgtgttggtg atgaaataga agttatggga ccatataaag aaactatgtt tactaaaatt
    52141 gaagaaatgt acaatgaaga aggagaagct atagaatcag ctcctcatcc tagacaaata
    52201 gtgaagttaa agctatcagt aaaagttggt aaagattata tgcttagaaa agttatagaa
    52261 gaaaaggttg aagaataata aaaaattgat aaaaatagta ttagcccctt agaaattgtc
    52321 aataatgaaa ttatctaagg ggtgatttca tgtcaaaaaa gaagactcca tttttgaaaa
    52381 aagtgggaaa gagaagctgg tgtatattta ctataatttt aataatttat agtgttttaa
    52441 tttataggct agttgatata caagtattaa aaggtgataa atacaaacaa agtgttgaat
    52501 cacaaagcgt tgagaaagtc gagttaaata gtggaagagg aataatctat gatagaaaca
    52561 acaagaagct tacagataca agtaaatctc aggtattaat tgttgaaaaa gaaaaattaa
    52621 ataataacta taaaattttg gagcttatta agaaagctac taagatgaat gatttagata
    52681 tatataaagc tgtacaagag caattgacac gccctataat acagattcag actaaaaata
    52741 tcgataagag tatgaaaaaa gaacttgaga aaaatggaat aatggtagaa gaaaaaacta
    52801 tgaggtacgc aaaagatggt ttattatcac atacaatagg atatataaaa gaagatgata
    52861 aatcaggtca atcaggaatt gaaaagagta tggatagtgt acttagaaat tcaaatgaaa
    52921 agtatataag tgcttttaaa gctggagatg caggaaatga aaaatctcta aacatactaa
    52981 agggaagtgt aaaaactgtt gataataagg ataaagatag acatctaaaa actactattg
    53041 attataacat tcaaaaaaaa ttagaacaaa tactaaataa agaagaaaat cctacagcag
    53101 caataatctc ggaagcatct acaggtgaaa ttcttgcaat gtgctctaga cctaattttg
    53161 accaaaatga catatcaaaa agtttaaaag gaaaaaatgg agaatttgaa aatagagtta
    53221 taaaagcgac atatccacca gggtctgtat ttaagatggt agttttattt tctgcacttg
    53281 aaaatggtgt tatagatgag aactatactt acaactgtac aggtaagact aaagttggga
    53341 atactaatga aatactgcga tgtaataaaa gagatggtca tggtttccaa aacttaagac
    53401 aagctttttc taattcatgc aatcctgcat ttttagatat agctatgaaa cttggaaaag
    53461 aaaagatact caaatctgct gaaaaattac atttatttga aaaggttgat attggtcttg
    53521 atgaagaaaa aattagagaa gctccaaaaa acatatcaat aagaaattta gctataggtc
    53581 aagaaaatat agaatttaca ccacttcaaa taaatcaaat gactcaaata atagctaata
    53641 atggaacatt caaaccacta tacttatata aaagtcttgt ggacaataat atgaatacaa
    53701 taaaaactta taagtcttca aaaaaagaag aacttatttc tccttatgtg tgtacacaag
    53761 ttaaagaata tatgaagtct gtatctagga ttggtacagc taaagaccta aaagatattg
    53821 aaggtggttg tggagttaaa actggtacag ctcaaagtag tttaaataaa aaagctatag
    53881 accatggatg gataacagga ttttatcctg aagaaagacc taagtatgta ataactgtgt
    53941 tagtagaagg aactcaaaaa ggaaataaat ctgcaacacc tatatttaag gaaatatgtg
    54001 aaagtataaa ataaattatg tactataaaa tttaaggata tatcgtaact ttgattttag
    54061 atagaaaatt ttgtctaatg atgtacgata tatcctttta aaatatagta ttaactttct
    54121 tgaacaagct ctttttctag ttttttaaga gctttttttt cgattctaga tacatatgac
    54181 cttgatatat cgagtttttg tgcaatttct ctttgtgtct tatagccata aggagttaac
    54241 ccatatctta actggacaat ttctctttct ctaggagtaa gaattttgtt tagttgttcg
    54301 taaagttttc caacttgaac ttttagttca acttcatcaa gaacatagtt agcctctgta
    54361 ccaagtatat ctaaaagact tatttcatta cacactactc tttatatgtt attggtatta
    54421 ctggtcttca tgtaatttca aatggaccat taattcaaaa gaatctagtt tttgttcttt
    54481 agacttataa taaactgctt tatcaattac agattttagg agtttatttt ttccaaggga
    54541 atctgaatca tggtaaagtg ataacacagt tttaatatct tctattattt tagaattgtc
    54601 ttttttaaca gtacttaatc tattttttac tgtcttaatt gaatctttaa tttcttcagt
    54661 tcttgaactt atattgttgg acctttcaat aaatgtatct tcatcatata ttcctcgttc
    54721 tagtaaatca tataacttat ttttttgctt gcctaattct tttaattcag tttctaagcc
    54781 ctttaagact cgtttttgaa attctatatt actatcttta ttatttttat ctaagtctat
    54841 atcagatgct tttaattgat atttttttac agtgtcttct aaagatttta aaatggcagc
    54901 ttcaataaat tcaaatcgag aacttttatt gcaaccagga tgataacaaa ttatatagtc
    54961 gtggtctgca tatggtctat atactaatgg tctaccacaa tttttgcaaa tcactattcc
    55021 tgcaagtgga ttttttattt gattaccata gctgacatga ccattttttt tgagtatatt
    55081 ttgagctttt ttccatgtta tttcacttat taaaggttca tgctttcctt ttgcctcaat
    55141 ccattcatct ttaggtctta gtttaactgt ttttatttta tttggatttt tagatttttt
    55201 gtagtccttt ttttgccact gaatgtagcc acaatatacc ttatttttta ttatatttgt
    55261 aattgcagaa ttgtaccaat tagcacctgt agcagtttta atgcctaagt tatttaggta
    55321 ttttgatatt ttactacaac ccatatcttc attaatatat aagtcaaaga tagtcctaac
    55381 tacatcagca tccttatttg gtacaagtat tctttctttt ctaccagttg ttacagcatc
    55441 atatccaaat ggagcagagg ttcctatgaa attcccttct tcaactgatt ttattctacc
    55501 tctttgcatc cttcttgata taagttttaa ttcttttctt gccataaatg cttcaaattc
    55561 tgaatactcc tcatcaaatt catctgttag gtcgtatgtt tttctaggtg tgataatttt
    55621 ggtcttactt tctttgaatg tttctaatat gataccttgg tctttcatgt ctccacgacc
    55681 aagtctgtct aagtccatta ctaagactgc atcatattta ttttcttcaa cttcttttag
    55741 aagttcaagc attttgggtc tatgtattat actttcacca gaaactaatt cttcttttat
    55801 ctcaattaca tctaagtttt gttcttttgc tagttttagt agggttgatt tatgtctact
    55861 taaagtttca aattcgcctt gtttttctgc ctcttcatca gcacgggatt ttcttaagta
    55921 tatggcaaca ctcataataa gtaatcacac tccttatgca ctattatata gtaaatcaat
    55981 agagtttaaa atatagaaaa taagcatgtc aaaagttaat ttaactttca acatacttat
    56041 tttctataat attaatgcct tataatcctt taatatcatt tagattagtt tttgcatatt
    56101 cattatccgg gttaagttca agcgccttat tgtaagattc tctagcttta ttaaattctg
    56161 atttactcat aaaaataaca cctcttaaat tgtaagcatc actagcgtct attttataag
    56221 cttcattatt tttatctaga tattctaagt atttatcaat tgactttaaa gcctcatcat
    56281 atcgttgtaa ctctatataa atatatgatt gtgtataata tgcaagtgga tttttatcat
    56341 caatttctat agcttttttg atatcaatag cagctttatt ataatcctta tcttcatctt
    56401 tttctgtttc tgacctagcg attttgacat aattagagct aagagctctt ttaaagtctt
    56461 tatgccataa taatttatat tcattatctt cgattataaa atatctatca aaatttgcag
    56521 tatcttcttt attttcagtg atatacttta tagtatcagt ttcttttatt ttaacaagat
    56581 ttgtgtactc tttattagaa tatttgtatt tctctacttt attttcttca ccaagtttat
    56641 aatcttttag ggtttgaagt ttaaataaag tatcttgata ttcttcaaat ttttctagac
    56701 tgacgctttc cttactgtct ttacttaata aattatatgc tacttcgtag tctttgattt
    56761 ttatactatc aaggtacttt ttagctactt ctgtagcact ttcttttgga gcacatccaa
    56821 ccattgatat cataattaaa aatgatacta atattgctgt tattttttta ctgattatca
    56881 taatataatc ccccttatat tgtctctcat taattttatt ttatcaccat attctaccaa
    56941 agtcattaat ttaagagtga tatttttcat taatattata ttaagtaata ataacctttt
    57001 aaatttatgg taacattttc ctgaaaagta ttatataatt taggtgaaaa tatttaagag
    57061 aagtagatat tatgaaaatt aaaaaattat taacaatagg gttatcttta tctatattta
    57121 ttgctagttg ccctatatct gcaaatgctc ttgataaaat tgaaccaatt aaaggtgcag
    57181 ataaatacga aactgctggt ataattgcag acaaataaaa ctatactaca gctatattaa
    57241 ttaatgcaga cagtacaatg tctgatggat taagtgcaag tggacttgca ggagctataa
    57301 acgctccaat tcttttgact aagaaaaata acataccaaa tgctacactt aaaagattag
    57361 aaaaggcaaa aaaagtatat ataattggag gagaaaactc tattgataaa tatactgaaa
    57421 ctgttctaaa aggcaaagga atagagatta aaagacttca aggtagtgat agaattaaga
    57481 caagctataa tgtcgcaaaa gaaataaact caattaacaa agtaaataaa gtcatattga
    57541 ctaatgcttt taaaggagag cctgatgcaa tgagtgctgc acctgtagca gttagagata
    57601 aagcagctat agtattaact gatggaaaaa gtgtccattt aatacaactg gtgtagaaag
    57661 ttatgtaata ggtggtactt catcaatgaa tgacaaaata gttaaggata ctaattcaac
    57721 tagattaggt gggacagata gatatgatac taacaaaaag ataataaata aattttatag
    57781 tggagtaaaa gaattttata tagcaagtgg tactgattta gtgtatgctc ttgttggttc
    57841 taccattgct aaaaataatg ctattgtgtt agttggtaat gatagtaata aaagtgtatt
    57901 gaaaagtaca actaaattaa ctgctatagg aaatttgagt gatagtatat tacagcagtg
    57961 tttaaatgta acaaaaaata taggcgattc taatactgga agagaatata cagtagaaga
    58021 tgctgctaat gtagtgaaaa aactattagg tgtgaagaaa ttttattacg aacctaatta
    58081 ttcagaagat gaagatttaa cttacaatct atagagtgga gttcatgtac aaaagattat
    58141 cctcccaacg attcgaaggg aggtgagttc atggataact ttttgtttaa tgttttagct
    58201 agtttaacag ctagtgtggt agtttactta atcagtaaac tattcaaaaa agcaaaaagc
    58261 cactctcgca caaagagtga cttacgagtt gaatttaaat ttatatttaa atccaaaaaa
    58321 taaactctgt ttatgatgaa ctccactcta acgcaaaata gattgtagtt cttcttgctt
    58381 ttattatacc acaaaacatt ataaataaaa ctgttgttta taaaaaaatg caaagtaaaa
    58441 ttttttataa acaatgataa aaattttatt atagagctat taacttatga aatatctgta
    58501 aatgagcaga tattttataa gttaatattt attgtttaca taaaatattt ggtaaaaata
    58561 agtaagtttt atatgttata atagttgtag caagaataat aatctaaagt ggcaagcatg
    58621 tcagctggtg ttatatcagt ttatatttat gataaaataa aaatctccca aacgccaata
    58681 agaatgattt aaaaaaataa tattttcact taaaaataat cactctttat aggagtaaat
    58741 tattttcttg attttattat actacaaatt ggtacagata tttaaaaata atatattcat
    58801 aatattataa atagttttgc agtgagcgat atttttgata aaatagggct taacagttga
    58861 aacataaggc attgagggta tatgataaat attatcattt gcactactcg tggttcactg
    58921 caaatttgag agaattgtat agatgtaagt gttggaaata ctcaatttat tttggggttt
    58981 tatattaact aagtggtatg taaaggcaat aaaacctgct attcttggta gaaattcatt
    59041 tggttttata ttaactaagt ggtatgtaaa gtttgacgtg ttaagtgata tagatagaag
    59101 aatatgtgag ttttatatta actaagtggt atgtaaagtt tgtttctctg taacatgatt
    59161 atcattcata attaaagttt tatattaact aagtggtatg taaagagagg ataaattgtt
    59221 tacatagtac cagttgggtc ttgttttata ttaactaagt ggtatgtaaa gttgcacttg
    59281 aatagttctg aagaacttaa gaactatggt tttatattaa ctaagtggta tgtaaagagc
    59341 tttacaatat caaagaaaaa ttgaaacatc tatgttttat attaactaag tggtatgtaa
    59401 agggaaaatt atgggaaaag tttacagata tcatgagagt tttatattaa ctaagtggta
    59461 tgtaaagtaa aaataaacct cctaaacaaa tatttcacgc atagttttat attaactaag
    59521 tggtatgtaa agcttgtaat cgctgaataa agatttctag cttcgttatt gttttatatt
    59581 aactaagtgg tatgtaaagg actctgccat ccctgtaacg aacagcctta cagcaagttt
    59641 tatattaact aagtggtatg taaagactta tctgtaaccc ttatatgttc aaattcatag
    59701 ccggttttat attaactaag tggtatgtaa agtatttcta gcgacttagt cggttgctcc
    59761 atttagcgtt ttatattaac taagtggtat gtaaagtagg attgacacgt tacgaccgtg
    59821 caacgttgga ctggttttat attaactaag tggtatgtaa aggcaataat attatcaatt
    59881 ttaatatttt gtttctcatg ttttatatta actaagtggt atgtaaaggg ttctggcaag
    59941 tcacttcatg ttgctagaga tatttgtttt atattaacta agtggtatgt aaaggttttt
    60001 tcttcgtaac tttgagcctt acatccacaa agttttatat taactaagtg gtatgtaaag
    60061 aacaaaaaag atacatctgt aactaatttt tatactgttt tatattaatt aagtggtatg
    60121 taaagacaga tataatgtca aaataaggtt aggactttta aaagcatagc catatcagta
    60181 aaaatttaat tatcatttaa aaaataaatt tttatttaaa gaatacacat aaatatttcc
    60241 agactcaact ctcttaaact attttcatat cttttagtag atacctttgt tgttttttaa
    60301 tctttagcaa cttgttcttg agtgaacctc ttattttttc taagttattt caagcttttc
    60361 aaaaaattct ctatttatat tcatcacaca tataatacaa ttaattcagt tttaattgcc
    60421 aatatttagt ttttctgtat ctgataaacc aagaatataa tcagtatata aaccaaaaat
    60481 tttagcaaat attattaacc catcttctct tgttggtctt tcaccagact ctattctatt
    60541 cataacactt gtatttatat ttgttttttc aagcaattct ttttgagaac tattcatatt
    60601 ttccctaata tatttaatcc tttgatttac atccatacat aattatctcg aaaagtttct
    60661 gctttggaaa tattgttatt gaaatataga aattaatgtg aatatatgaa aaagatacac
    60721 tatgatttta taaaaaagaa gacatacata aaatttatac ttacaaagtg ttgcaaaaga
    60781 gttaggtttt aaaaatgcat caacttattt taagtacagg aaagggtaat attcaattag
    60841 agttgatata ttacctataa actatctaag atacttgatt atgatataga aaatttaatt
    60901 aagaggatga taaggatgaa taatttacaa gtaatataaa gaaataataa gagaatttta
    60961 aatacatagc aactagcaga tatatatgaa aatgatgtaa acaatatata aactagcttc
    61021 aatagaaata aagagaatgt aaattatgtt ttattacaat tcgagtattt gaaagaattt
    61081 aagaactaac ctactaatag tcagttgatt tcaaaacatt ctagtcaatt atacatttgg
    61141 accgaaagat gtgcaaatag acactgcaaa atattagata ttgataaaac ttgagaacaa
    61201 tttgacaatt tagagaaaat atatttcaag attaagcgag agaaactaag ctgtatagaa
    61261 gatgtattaa tagaaaattt aatatttaag gatacaaatt aatcaagcaa taatatggca
    61321 ttagaggtaa aaatagaagt taaaataata aaagatgttg ttatattaaa tataacacat
    61381 tggaaaaaag aaacaagtct attaacagtt aaaattgcta gaggtcaagg tgatttaagt
    61441 tatgtaaaat attttactaa tgagagctaa gagcttctta acaagagttt tggtgtgtct
    61501 attgaaataa tgttaacaat taataaaaga cgaatggcag aagaaggcgt atgtaagttt
    61561 aaaagagata agttaaacaa actagatact atataagagg ataagaaact tatagaagaa
    61621 tatgttactt ttgtaaagga tttagcttta atatatagaa tataaagtga ttttaagtaa
    61681 aaattgtaac aattaaataa gtataagaca agtttatgag ttcatatttt ataacaaggg
    61741 gtgatgttat gaaaatacta caacaattta aaccatacaa atatgataaa gaaaaaaata
    61801 tagaatatga tgaaaaaatt atagctaatc aaaacacaga agtttatata gtatatccac
    61861 gtatatctga tgaagaaaat aggagaagat gggaagagtt caatgaagta gctaaagaag
    61921 tagctattaa tatagctatg aagaaagttg agacagtaga aagttaagag aatagagaag
    61981 ttgctaatac ttagcacttt ctcatagaag aaaattggac aactattttt aaataattag
    62041 gaataaaaaa gttaaatcat atttgaaaaa aggcgataaa attgttgagt attgttatta
    62101 gtaataagaa tgtattaact atgtattaaa agaaattata agtttttagt aaatatagta
    62161 tttaaggata ttaaaactgg taaaaaatat catttagact attaaataaa aaagtgctgg
    62221 tcaaagtaag caacacaata aataaaaaaa cttatagaaa ttataagata aaaagatgag
    62281 atttgaaatg gttgaagtta atataaaaat taaaggtgaa gtaagtgaaa taattgactc
    62341 aattagaggt atatttaata gctttgatga agagttgata ttagatataa aatcagagta
    62401 agaatataaa ggtagatacg tagaaagtta taagtaattc aagacttaaa ttaatttcta
    62461 gtttttttaa tgcaaaaatt taataaattt caaaatataa taattctgac atctaaaaag
    62521 agctattaaa aatgaataat attgactatt aagatatgaa tagtgtacat atattaaatt
    62581 aagatatcca gtaggttaat tttatataat gatggggctc aaattgaaga ggcgatataa
    62641 aaatgtatta atatatataa atataagaac tataataaat attaactgta aaattaaatt
    62701 tatagagtta tatttaacat ttaatgaact gaaaaataag ttagcaaata atgaaaaaac
    62761 aacagtaata aatgagaata acaaagcatt ttcaggatta gtggattttt ctaattgagc
    62821 tttaatccta gtttgttcaa tttcgatatt tctaatatta ttagtattat caatttattt
    62881 gtaataatta tcagtttttg cgaaatattt ttgataattt atagaacttt atatattttc
    62941 cttgaagtat ttttcgccct tattgtttgg tttccaattt taaaactcat aaactcccaa
    63001 taaattcatg tataatatac cttaatttat aatatattta agagtttatt tcagtaattt
    63061 ttcgaacgat tttgatatat ttcatttata gttttcaaaa acttatacaa aaaagagtgt
    63121 ttcatttttc taaacactct ttttataaga atttgaataa taatttttta aaaattgata
    63181 atatggttat ttctttttca agtttatagt ttcctcattt ccattccaag atataattag
    63241 cttgtaacta gattctctat ttgaataaag acctggtctt tgtattttta ttgctttttc
    63301 ttgacctgct tctaaaattt cattttttac aactacaact gcatcttcac catctataaa
    63361 ttgataggaa taattaaatt tttgttcaac tacatcttta ctatttttat attttatttc
    63421 taattttgag tgtccttcag tagttacaaa tttcgctttc caattttctg attctccaat
    63481 aaagacattt ttatcatttt ttatattaag tatagctatc acagagatta ttaaaactat
    63541 aagaatacct ataaaaaatt ttttcctcat atcaatccct cctttgaata attataacat
    63601 gagaatagtt attcaatact aattttaaaa atattgtgga tttatattct attacaatag
    63661 aatgtctgtt ataacaaata aaaagtaggg attaatatga attataggtt aaaaaaatct
    63721 gtaacaacta ttttatcttt actcataatg acaatactat taccaacaaa tgcaagtgct
    63781 agtaaggtag aagattcatc tatgaaagta atgtattcag agaaacaagt aacagacatg
    63841 gacaaattat acgatatggc taaaaatgga attactgatg ttaaaagttc caatgaaaaa
    63901 ggtataatca aaaatgcaag aacaggagag tccatatacg ttgattctat atcaacaact
    63961 caattattag aagttaaaca atctaaaaat ttaacatcaa agatatattc taagacagtt
    64021 ttttgatact aatacaataa agcctcttag aaaagcaagt atatcagaag atggatggga
    64081 taaaactggt ggtgtaaaag catctggtac agttacatat acaatatcaa aaggagatgg
    64141 agcatttcaa tatatttccg ttagtaaagt ttctggaaac tggtctattg cagacggcca
    64201 gattcgtgta acaaatagaa gggtgcatat aaatcaaact ggatttattt ctaatgggct
    64261 aggaattaag cagcaacaaa agaaagagcc aacatcaaat tctttctcat ataatgcacc
    64321 ttctagttgg aaaccagtag gtagagattc agttcaatac aattgtgggt ttgaatcaat
    64381 tgcaacatta gtaagaggag gaagttcttg gtcattcaat ttcattgttt aaccttaaaa
    64441 tattttaagt gaattttatt aaaattaaag atatacatat actttaaaat tagaaaatgt
    64501 aaattaattt gtgtttttaa agataattcc taagttatag caac
//
LOCUS       DAMJWS010000012        59098 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMJWS010000012 DAMJWS010000000
VERSION     DAMJWS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.012"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            236..1069
                     /locus_tag="PC399_000826"
     CDS             236..1069
                     /locus_tag="PC399_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2591702.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1162..2094
                     /locus_tag="PC399_000827"
     CDS             1162..2094
                     /locus_tag="PC399_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2591703.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2146..2628
                     /locus_tag="PC399_000828"
     CDS             2146..2628
                     /locus_tag="PC399_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2591704.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2640..3509
                     /locus_tag="PC399_000829"
     CDS             2640..3509
                     /locus_tag="PC399_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2591705.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3552..4307
                     /locus_tag="PC399_000830"
     CDS             3552..4307
                     /locus_tag="PC399_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2591706.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4349..5170
                     /locus_tag="PC399_000831"
     CDS             4349..5170
                     /locus_tag="PC399_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HDF2591707.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5175..5936
                     /locus_tag="PC399_000832"
     CDS             5175..5936
                     /locus_tag="PC399_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF2591708.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLA
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6097..6354
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6467..6706
                     /locus_tag="PC399_000833"
     CDS             6467..6706
                     /locus_tag="PC399_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2591709.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7010..8005
                     /gene="ilvC"
                     /locus_tag="PC399_000834"
     CDS             7010..8005
                     /gene="ilvC"
                     /locus_tag="PC399_000834"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF2591710.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8058..9746
                     /gene="ilvB"
                     /locus_tag="PC399_000835"
     CDS             8058..9746
                     /gene="ilvB"
                     /locus_tag="PC399_000835"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF2591711.1"
                     /translation="MSGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            9957..10634
                     /locus_tag="PC399_000836"
     CDS             9957..10634
                     /locus_tag="PC399_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2591712.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10808..12844
                     /locus_tag="PC399_000837"
     CDS             10808..12844
                     /locus_tag="PC399_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF2591713.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(13035..13601)
                     /locus_tag="PC399_000838"
     CDS             complement(13035..13601)
                     /locus_tag="PC399_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2591714.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            13889..14824
                     /locus_tag="PC399_000839"
     CDS             13889..14824
                     /locus_tag="PC399_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2591715.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15289..16503
                     /locus_tag="PC399_000840"
     CDS             15289..16503
                     /locus_tag="PC399_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2591716.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETMNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(16923..17444)
                     /locus_tag="PC399_000841"
     CDS             complement(16923..17444)
                     /locus_tag="PC399_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2591717.1"
                     /translation="MKFKRLRTSENKLCYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            17800..18597
                     /locus_tag="PC399_000842"
     CDS             17800..18597
                     /locus_tag="PC399_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2591718.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGIKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            18707..19297
                     /locus_tag="PC399_000843"
     CDS             18707..19297
                     /locus_tag="PC399_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF2591719.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            19363..21060
                     /locus_tag="PC399_000844"
     CDS             19363..21060
                     /locus_tag="PC399_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2591720.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            21274..22113
                     /locus_tag="PC399_000845"
     CDS             21274..22113
                     /locus_tag="PC399_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF2591721.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            22630..24486
                     /locus_tag="PC399_000846"
     CDS             22630..24486
                     /locus_tag="PC399_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2591722.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    24569..24809
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    24875..25110
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            25195..26412
                     /locus_tag="PC399_000847"
     CDS             25195..26412
                     /locus_tag="PC399_000847"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2591723.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            26529..27023
                     /gene="cdeM"
                     /locus_tag="PC399_000848"
     CDS             26529..27023
                     /gene="cdeM"
                     /locus_tag="PC399_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF2591724.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGENRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            27288..28553
                     /gene="hisD"
                     /locus_tag="PC399_000849"
     CDS             27288..28553
                     /gene="hisD"
                     /locus_tag="PC399_000849"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF2591725.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(28756..29508)
                     /locus_tag="PC399_000850"
     CDS             complement(28756..29508)
                     /locus_tag="PC399_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2591726.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(29626..29895)
                     /locus_tag="PC399_000851"
     CDS             complement(29626..29895)
                     /locus_tag="PC399_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591727.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            30370..31464
                     /locus_tag="PC399_000852"
     CDS             30370..31464
                     /locus_tag="PC399_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2591728.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISNITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            31466..32416
                     /locus_tag="PC399_000853"
     CDS             31466..32416
                     /locus_tag="PC399_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF2591729.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKVDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            32409..33593
                     /locus_tag="PC399_000854"
     CDS             32409..33593
                     /locus_tag="PC399_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591730.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            33606..35102
                     /locus_tag="PC399_000855"
     CDS             33606..35102
                     /locus_tag="PC399_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2591731.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEECMKICEDFDFNIDPHMVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVND
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            35125..36075
                     /locus_tag="PC399_000856"
     CDS             35125..36075
                     /locus_tag="PC399_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591732.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            36096..37058
                     /locus_tag="PC399_000857"
     CDS             36096..37058
                     /locus_tag="PC399_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2591733.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            37430..38062
                     /locus_tag="PC399_000858"
     CDS             37430..38062
                     /locus_tag="PC399_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2591734.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            38473..38565
                     /gene="kdpF"
                     /locus_tag="PC399_000859"
     CDS             38473..38565
                     /gene="kdpF"
                     /locus_tag="PC399_000859"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF2591735.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            38606..40288
                     /gene="kdpA"
                     /locus_tag="PC399_000860"
     CDS             38606..40288
                     /gene="kdpA"
                     /locus_tag="PC399_000860"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF2591736.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            40313..42370
                     /gene="kdpB"
                     /locus_tag="PC399_000861"
     CDS             40313..42370
                     /gene="kdpB"
                     /locus_tag="PC399_000861"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF2591737.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            42389..43015
                     /locus_tag="PC399_000862"
     CDS             42389..43015
                     /locus_tag="PC399_000862"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF2591738.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVEDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            43252..44160
                     /gene="cysK"
                     /locus_tag="PC399_000863"
     CDS             43252..44160
                     /gene="cysK"
                     /locus_tag="PC399_000863"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF2591739.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            44187..44777
                     /gene="cysE"
                     /locus_tag="PC399_000864"
     CDS             44187..44777
                     /gene="cysE"
                     /locus_tag="PC399_000864"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF2591740.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            44937..45125
                     /locus_tag="PC399_000865"
     CDS             44937..45125
                     /locus_tag="PC399_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2591741.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(45179..46048)
                     /locus_tag="PC399_000866"
     CDS             complement(45179..46048)
                     /locus_tag="PC399_000866"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF2591742.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDVINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(46245..47489)
                     /locus_tag="PC399_000867"
     CDS             complement(46245..47489)
                     /locus_tag="PC399_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591743.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYNMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            47780..48091
                     /locus_tag="PC399_000868"
     CDS             47780..48091
                     /locus_tag="PC399_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2591744.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      48184..48276
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            48376..49170
                     /gene="thiD"
                     /locus_tag="PC399_000869"
     CDS             48376..49170
                     /gene="thiD"
                     /locus_tag="PC399_000869"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF2591745.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            49194..49982
                     /gene="thiM"
                     /locus_tag="PC399_000870"
     CDS             49194..49982
                     /gene="thiM"
                     /locus_tag="PC399_000870"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF2591746.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            50005..50637
                     /gene="thiE"
                     /locus_tag="PC399_000871"
     CDS             50005..50637
                     /gene="thiE"
                     /locus_tag="PC399_000871"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2591747.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKQYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            50776..51078
                     /locus_tag="PC399_000872"
     CDS             50776..51078
                     /locus_tag="PC399_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2591748.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(51144..51893)
                     /locus_tag="PC399_000873"
     CDS             complement(51144..51893)
                     /locus_tag="PC399_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591749.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(51887..52849)
                     /locus_tag="PC399_000874"
     CDS             complement(51887..52849)
                     /locus_tag="PC399_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591750.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(52853..53311)
                     /locus_tag="PC399_000875"
     CDS             complement(52853..53311)
                     /locus_tag="PC399_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2591751.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            53667..54023
                     /locus_tag="PC399_000876"
     CDS             53667..54023
                     /locus_tag="PC399_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2591752.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            54095..54811
                     /locus_tag="PC399_000877"
     CDS             54095..54811
                     /locus_tag="PC399_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591753.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            54804..55628
                     /locus_tag="PC399_000878"
     CDS             54804..55628
                     /locus_tag="PC399_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591754.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYVFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            55640..56839
                     /locus_tag="PC399_000879"
     CDS             55640..56839
                     /locus_tag="PC399_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591755.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEDSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILSSKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            56848..57660
                     /locus_tag="PC399_000880"
     CDS             56848..57660
                     /locus_tag="PC399_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591756.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTELWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            57671..58939
                     /locus_tag="PC399_000881"
     CDS             57671..58939
                     /locus_tag="PC399_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591757.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT        23442 a         6090 c        10209 g        19357 t
ORIGIN      
        1 aatgacattt taactagaat taattgtaaa aaacactata taatactcaa ttttcaacat
       61 tctatacaat tatacagaaa ttatacagca taataatcac cgaaataaca aattttaaaa
      121 attaagcaaa actttttgtc gaagttcaac gtctatatat atgtagtgta aatttttggg
      181 ataaaatact acaaaaatat aaatgtaaaa atatagtaat attggaggtc gaacgatgag
      241 aaataaaaag aaaaaaataa atagaaagaa gttgtatttg cttttaagtg ctattgtagt
      301 gtgtctggct tttatagtag ttggtgttcg cgtcagctct ttaaatatga aggaaaatga
      361 aattagtaga aatgcaaaac tgtcaagttc tactattaca gatatagtat ctaatacatc
      421 aatagaagtt agtaaagggc caagtaaaag ttctggaaaa atagcataca taactataga
      481 tgatggtcca tctaaattta ctgacgagat gataaaaact ttaaataaat acaatgttaa
      541 agcaactttt tttatgatag atgggaatat gaaggaatat ccacagcaag ttaaaaatat
      601 aattaaaaat gggaacacag caggttttca tagtgtatca catgatattc acaagcttta
      661 tgttactagt acatcagcaa aagaggaatt tgatacaaat gatcaaactt tttataagat
      721 aactggtaaa cattcaaaag taataagaat accttatgga agcaaaccat atacaccaca
      781 agcctcttat caggctttag ttgatgctgg atataaaatt tgggactggg acttagatac
      841 agaagactgg agatctaatt cttctcaaat tgtacaaaat gtaaaaaatc atattaagaa
      901 tagaaaaggt gaagataaag accaacttgt agttttaatg catgagaaaa aacagagtgc
      961 agaagcttta gattcagttt taaaattctt atcagatgaa ggttatgaat ttgcgccagt
     1021 agatcaaaac caaataccaa aaaattattg gttacgtaat ttagagtagt taaaatataa
     1081 gaatattgat tgatattttt agaaattgag aataaaaatg agtgaggtgt aggtatattg
     1141 aataaaaaac tttatatagg aatggtcgga atattgtctt tgatgatggt tggttgtaat
     1201 aaaagtttag caaaagtaaa tgatgtggaa atcacaaaag aacagtacaa aaaaacaaaa
     1261 gcagttttat ctgctactaa taattatata aatgggcagt ctttagatga attagaaaaa
     1321 actttggata aaaaaggtag aaataaatta gaaaatgtta taatatcgtt tatggtggat
     1381 aatgaacttt tatatcaaga ggctaaggat aaaggactaa ctccaagtaa aagtgaagta
     1441 gactcaaaat atcaagaact agaagataag atgaatttga atacaagcta taaggaaaaa
     1501 atggacaaag caggtgttga taaagattat ttaaaacaag aaatatctag agatttagca
     1561 atagataaaa ataaaaaggc ctttgaagat agaataaaca taagtgataa tgatatggaa
     1621 gcctactata caagtcataa aaaagatttt aatgtagaag aagtaagtgc ttctcagata
     1681 cttatatcga ctttagataa aaataaaaaa gaagtaagca aagataaaaa agaagctttg
     1741 aagaagaagg cagataatat attgactaaa atcaaaaatg gagaaagttt tgaaagtttg
     1801 gctaaaaaat attcggatga taaagcaact ggaaaaaatg gaggacagct aggatatttt
     1861 acaaaggatg ataaaaatgc tgagtttaca aaggaagtgt tcaaattaaa gaaaaatgaa
     1921 gtttcaaatg tgtttgaaac tagttatgga tatcatatag tcaaagttac tgataaaaga
     1981 gaaagacaaa aaagttttaa tgagtgtcag agtttaataa gagaatctat tttaaatgaa
     2041 aagtatattg agcatataaa aaaactgaat gaagatgcta aaatagacag atagaatttc
     2101 taacagctca atcctagcct gtaatcggag ggagatttta caggaatgtt taagagttta
     2161 aagagaacga aagttgagaa atacattatc gataataaag actcttttta tagaatagca
     2221 tatagttata cgaagaatga agaagatgcc ttagatgtgg ttcaagaagc catgtataag
     2281 gcactatact cagtggaaaa tattaaagaa gtaaattata taaagacatg gttttataaa
     2341 attttagtta gaacgagtat agattttatt agaaaaaacc gtaaatataa taatatgaca
     2401 gatattgacc ttatagatga gactggagag tatgataaat atactgattt ggatttaaga
     2461 agagctttgg aagaattacc tattgaatac aaatctatta taatattaag attttttgaa
     2521 gatttaaaga ttgaagaagt agcaataata ttggatgaaa atgtgaatac tgttaaaact
     2581 aggttatata ctgctttaaa aaaactaaaa ttaaaaattg aagagtaggg ataaaaagaa
     2641 tgacaagcaa agaaaggtta aataagttaa aagatgagta tcataaaatt ccaataccca
     2701 agaaattaga tactataata aatcatgaaa aaatagaaaa catatatcga gagaaaaata
     2761 aaaaagtaaa tagattaaga tttaaagttg caatagcctt tgcatgtata tttacagtat
     2821 tagtaaatat aagtcctgta tttgcagaca atttttctaa gatacctgta attggagcaa
     2881 tagtagaagt aataactata aaaaattata gtttaaaaag tgaaaattat gaagctgaga
     2941 ttgatattcc taaaatacga ggtttaaaag ataaaaactt agaacaaaga ttaaacagta
     3001 gttttatgga agatggaaaa aggctttatc atcaattcca agagagaatg gagaagatac
     3061 aatcatcaaa gaataaagga tataaatctt taagtttgag ttatagtgta aaaaataatt
     3121 ctaaaaaatt tctatcaata gagatgacaa aaaatgagat agaggcttca tcatatgtga
     3181 gtaaggtaca ttatactatt gataaaaaaa gacaaatagt tttgactttg ccaatgcttt
     3241 ttaaagatga taagtatata aaagttataa gtgataatat aaaagaacaa atgagagaac
     3301 aaatgaaaaa ggatagtaca aaatcctatt ttatagatca aaaaaaagac ttgcctgttg
     3361 aagattttaa aacaattaat aagtatcagg atttttattt taataaaaat gaagaccttg
     3421 taatttgttt tgatgaatat gaagtagcac ctggttatat gggcgcagtt gagtttgtaa
     3481 taccatataa agtaataaaa gatttgtaaa ttgaatagta gtttatatat aaatctttta
     3541 aggaggacaa tatgactaca tataaaaaac ttaaaaaatc tatatttaaa attgctatat
     3601 tttcaatatg cttattagtt tcattagtag atacaaaccc tatatttgca ggtagttcct
     3661 ttaatgaaac ttcaaatagt gatacgataa aggtgttaaa tgctaagagt atcaacttta
     3721 aacaagacaa ttatgaagtg aatgtgagta ttcctaaaat aaaaggttta aaagataaaa
     3781 ctttagagaa taaattaaac aatgagtttt tgcaagatgg gaaaaaaatt tacagtgaat
     3841 ttcaaaataa aatgaaaaag gaaaataata agggtcatag atacctaaat gtaatccata
     3901 atgtcaaaaa taattctaag gactttttat caatagaaat gacaaaagaa gagatagaag
     3961 cttcctcatc tataactaag aagcattaca ctgttgataa aaatagagga atactattaa
     4021 atttaccaat attttttaaa gatagtagat atattagagc tataagtgat aatataaaag
     4081 agcagatgag aaatcaaatt aagaaagatg atacaaaatc ttattttata gaccaagata
     4141 aagactcgcc agtgaaagat tttgaatcta taaataagta tcaagatttt tattttaata
     4201 aaaatggaaa tcttgtgatt agttttgatg aatatgaagt agctcctggt tatatgggag
     4261 cagtagaatt tgtgatacca aataaagtaa taaaaagact aagatagata tatagaagat
     4321 ttttagatag actggtaagg agaattatat gaatatatat gaacaagcaa taagattaat
     4381 agaaaataat gaggatttcg catttgctac aataacgtct cattcaggtt caacaccacg
     4441 tgaaactgga gcaatgatga ttgtgaaaaa tgattctact atatttggct cagtaggagg
     4501 aggtagtgta gaagcagaat gtataaaaca tgcaattaat gttataaaaa acagagaatc
     4561 aatgttgtat aaatttactc taaacaaatc tgatgtggca aaactaggaa tgatatgtgg
     4621 aggtactgga gaaatacaaa tagattttat agatagtaaa ttaaaaagta atatagaaaa
     4681 atttaataag agactaaagg aaaatacaag taaagcatat atatttggtg caggtcatat
     4741 ttcaagagat gttgctgtga tactatctct tttagaattt agaacagtag taatagatga
     4801 tagagaagaa tttgctaatc atgaaaggtt tccagaatca gaagtagtag ttttagattc
     4861 ttttgaaaac ataccagatt ttcccactga cgaaaatagt tatataatta ttttaacaag
     4921 aggtcatcta tatgattcaa gtgctttaga atgggctctt aaaagagagg ctggttatat
     4981 aggtatgatt ggaagtagaa caaaaattgg attgacttat gaaaaactca tgaaaaaagg
     5041 ttttaagaag gaagaattat caaaagtaca tgcaccaata ggcataaaat tgaatgctca
     5101 aacaccagct gaaatagctg tatgtatagc tgctgaactt ataaattgta gagctaataa
     5161 ggaaaaataa actaatgaac acagggagta aagatattga tactttaaag gttaatttta
     5221 gagggattga aataaaagca gttttagtat ataaaaatag aaaaaatatt tctataaaaa
     5281 tagatccatt tggcaaaata actgtaatga gtcctcctaa aatatccaaa aaaataataa
     5341 aagatataat tattgaaaaa ggagactgga tacttaaaaa gtcagaaaaa tgtaaaggaa
     5401 gagaggaagt atataaacaa agaatgttta taactggcga aaaatttctt tatttaggtg
     5461 aagagtacca tctagttata aaagaactgt tgagagattc tgtcaaaaaa agtaactgta
     5521 gaataactat taacgaatat caaatagttg ttcagacaaa tgatacaagt acagatttta
     5581 ttaaaaaatc tttaaaatct tggtataaga tggaaagtga aagaatagta ttagagagaa
     5641 tagattttct taaattaaat tgtgaaataa tgaagcaatt agttccagcg agtgttaagg
     5701 taaaagaaca aaataagaga tggggaagtt gtacagctca aaaaaatatt tatataaact
     5761 caaaaatatc tatggcaaga ttagatgtaa ttgattatgt aataatacat gagtttagcc
     5821 atttagcaca tatgaatcac tctaaaaaat tctatgattt agtaaaatcc attatgcccg
     5881 attataaaga taaagaaaat tggctaaaaa taaatggata taaaataata atctgaaaac
     5941 gattattcca aaatataggc gtaaagtata aaagaagtat tattgtgaaa aaaataaata
     6001 atctgaaaat tttaaaaaaa gtcattgaca ttgtaaatga tagagaatat aatctatatt
     6061 aacaacaaaa cagaattgaa atataaaatt aaaaaattaa aagcaatgac agagacaaca
     6121 atactaatag tgttatccac agagaaccaa tgttgctgag aattggtgtt aacagtagta
     6181 taatgatcac tctggagctt ttgtactgaa tatttttagt aagtacaaac gtatgtcaac
     6241 gttacatgac taaggttaag gtaattaatt acccgacctg aaaaaagagg ggtagatgta
     6301 ttttttatat ctacaaataa ggtggtaccg cggaatataa ctttcgtcct tataagtaat
     6361 atttatattg cttataaaga tgaaagtttt tttattttaa gtgatataaa taaatttaat
     6421 ccatataaaa ttaataaaaa aataataaaa ataaggaggg atttttatgg aaaatactgt
     6481 atatgctaaa ttaaaccaag gaatagattc ttttttaaga gtggctatga ctctaagaag
     6541 aagagaggtt gatatacaat caataagtat gactgtagat aatatgaata attcaggtat
     6601 aaaattaata gtaaatgaag aaaagacatc cttagattct gtcttaaacc atatgaaaaa
     6661 actacatgat ataagagaga taactgttga aagaatgaga cattaattaa atatatttta
     6721 taaaactaaa gataaatatg cttattttaa aataaaaaac taaagataaa tattaaataa
     6781 gaaaaatctt agattaaaat ataagtgtca ttgaatttat tagtttatca cagttaataa
     6841 atttattagt ttgttgtagc taataaaaac tgaaaattct aaaattataa ttgtaagatt
     6901 atataaattt agaatttaaa tctgttgaat aatattaaaa caatgataat gagtatgaat
     6961 ttctaataaa aataaatatc ataaaaataa gaaaaagtat tgggaggaaa tgaaaatggc
     7021 aagaatgtat tatgaaaaag atgtggattt agaggtttta aaaaataaaa aggttgcagt
     7081 tttaggttat ggaagtcaag gacatgcaca tgcacaaaat cttagagata acggtgtaca
     7141 tgtagtgata ggtctttatg acggaagtaa atcagcacaa aaagcaaaag aagatggatt
     7201 tgaagttaag agtgtagcag aagctacaaa agaaagtgat ttgacaatga tgttaatgcc
     7261 tgatgaaaag caaaagaaag tttatgaaga gagtgtaaag gataacttaa aagaaggtca
     7321 gacattagct tttgctcatg gattcaatat acattataat caagtacaac caccagaatt
     7381 tgttgatgtt gtaatggtag ctcctaaagg acctggtcac ttagttagaa atgtatttac
     7441 aaagggaagt ggagttcctg cactatttgc agtatatcaa gactacacta aaaaagctac
     7501 agagacagtt ttagcatatg caaaagggat aggagcaaca agagcaggtg ttttagaaac
     7561 tacttttaaa gaagaaacag aaacagattt atttggagaa caatcagtac tttgtggtgg
     7621 aataagtgaa cttataaaat tgggatataa aacgcttgta gatgcaggtt atcaaaaaga
     7681 agtagcatat tttgaatgtt tacatgaaat gaaactaata gtcgacctta tatatgaagg
     7741 tggatttgaa agaatgagat atagcataag tgacacagca gaatatggag attatgtatc
     7801 tggtaaaagg gtaataactg atgctgcaaa acaaggtatg caaaatgttt tagaggatat
     7861 acaaaatgga aaatttgcaa aagcgtggat taaagaaaat gaagaaggac gtgaaaactt
     7921 cctaaaaact agagaagaag aatataatac agaaattgcc gaagttggta gaaatctaag
     7981 aagtatgatg tcattcttaa aataaatata tattaaatgg attagtttga gggaaagagt
     8041 ggtgtatagt agtgcgtatg agtggagcaa aggtaatttt agaatgttta aagaaagagg
     8101 gaattgacac aatatttgga tatcctggtg gagccgtgat acctctttat gatgctctat
     8161 acgattattc agatgatttt aaacatataa ggacatccca tgaacaaggt ttagttcatg
     8221 ctgcagatgg gtacgcaaga agtactaata cagtaggagt ttgttttaca acatctggtc
     8281 caggagcaac aaatgccata actggaatag caacagcttt tatggattct tcacctatgg
     8341 ttgtaatttc agggcaggtg ccaactagtt tattaggaaa agattctttt caagaaattg
     8401 acataactgg agcaacatta tctatgacaa aacataatta tttagttaga aacactaaag
     8461 aattagttcc tacaataaaa gaagctttta gagttgcaaa ttcaggtaga aaaggacctg
     8521 tacttgtaga tgttccaaaa gatttatttt tagcagaaat ggattttagt ggtgaggact
     8581 atgatttatg tcaaatagat gattatatgg attataagag tgattttgat ctagatgatg
     8641 aaactaatat taagctttta aatgaagcaa tagatatcat aaaagaatct aaaaagccta
     8701 taatatatgc aggtggaggt gttaaatctt cagacagtga ggaaattctt gaaaaatttg
     8761 caactaagat agatacaccc gtactaaata cacttatggg gcttggaaat atagatagaa
     8821 agaatgaatt atctcttgga atggttggaa tgcatggaag tagagaaagt aatttagcac
     8881 tttctaattc agatttagta atagcgatag gtgcgagatt tagtgataga gtgataagta
     8941 aaagttctga atttgctaaa aatgcaaaaa taatacacat agacatagac ccatctgaaa
     9001 taagtaaaaa tatagaatct aatgtatctt tggttggaga tgtaaagcta gtccttagtt
     9061 tattaattga aagggttgaa agtaagaata atagcaattg gaaagaagag ataaagagat
     9121 ttagaaagag tgagggtgta caaacatatg aatttcatcc acaaaatata ctaaaaaaaa
     9181 taaatgaaaa atatgaaaca ttaaagaaac ccactgtggt agtaactgat gtcggtcaac
     9241 atcaaatgtg ggctgctaag tactggaatt ttaaaggtaa taaaagtttt ataacatcag
     9301 cagggttagg aactatggga tttggtttag gtgcagctat tggaactaaa gttggtaatg
     9361 tagataaaaa tgtagtttta gtcactggtg atggaagttt tagaatgaac tgtaatgaac
     9421 tagctacagt tgcaaattat aatgtaccta tgcttatttt attactcaat aatagaacat
     9481 taggaatggt aaggcaatgg caaaagttat tttccaatca aagatactct caaactgaca
     9541 ttaatgaaaa tgtggattat gttaaactcg taaatgcata taatattgat ggatataagg
     9601 tatccagtat ggaagaatta ggaaaggcgc tagatatgat agattttaat aaacctgtat
     9661 ttttgcaatg tgatatagat aaggattatg atgtttatcc tattgtagca cctaatgatg
     9721 cattggaaaa tttgatatgt aactaaaact tatactgaat attgataaaa ttttttatta
     9781 aatttttatt ggtattcagt ttttgttgtt tagagataga ttggcatttt ttatttttgc
     9841 aaataaatca atgactatat aattaagaac ttattaaatt ttaattatac aatttagaat
     9901 agtattacaa cttaagtaaa taataatttt gaataataat ttacggaggt attcatatgt
     9961 ttaaacacga taaagcatta ttaaaagaag taaaagtaga aagaccaaat cctcaatatg
    10021 ctgttttaat gcaagaacag ttgggtggtg caaatggaga gttaaaagca gctatgcaat
    10081 acttgtccca aagttttaga ataaaagacc ctcaaataaa agatttattt cttgatatag
    10141 cagcagaaga gcttagccat atggaaatgg tagcacaaac tataaattta ttaaatggac
    10201 atgatgttga ttataattca gttaatacag gcgaaataga aacacatgta ttaactggtc
    10261 tatcaccagt cttaataaac tcatcaggag ctccttggac tgctaattac gttacagtta
    10321 caggggattt agtagctgac ttactttcaa atatagcttc tgaacaaaga gctaaggtag
    10381 tttatgagta cttgtataga caaatagatg ataaatatgt aaaagaaaca atagatttct
    10441 tacttaatag agaagaagca cataatgctc tatttagaga tgctttaaat aaagttaaag
    10501 atacaggttc aaatagagat tttggagtta cagaagattc taaattatat ttcgatttgt
    10561 ctactccagg gccaaatcac gatactaaga tagatattaa tcctccttct tttgaaaaac
    10621 ctttaaaaaa ataatttgat attttttatg ttgttttgtt ttagtagtta taaaaattat
    10681 ttactaggag aattaattat atgaaaaatt aaatatgtat ggttaaaaat gggtatacac
    10741 ttattataag tgtatatcta tttgtttatt gcaaaacata taataaaata acaaatgagg
    10801 tggtattttg gacagtaata gaaaacctaa taatctaata aatgaaaaat ctccatatct
    10861 tttacagcat gcatataatc ctataaactg gtatagttgg aatgatgagg cttttaaaaa
    10921 agcaaaagaa gaggataagc caatattttt aagtgtagga tattcaacat gtcattggtg
    10981 ccatgttatg gaaaaagaat cttttgaaga tgaagaagtt gctgaaataa tgaatagaaa
    11041 ctttgtggcg ataaaagtag ataaagagga aagacctgat gtagatagcg tgtatatgac
    11101 agtttgccaa gccatgacag gtagtggtgg atggccaatg accataataa tgacaccaga
    11161 taaaaaacct ttttttgcgg gaacatattt cccaaaatat tcaaggtata atagacctgg
    11221 agttatagat ttattagaga atgtatctga aaagtggaat acaagcaggg atatattaat
    11281 aaaatctgga gatgaaataa tagaagctct taaggatgat tttggtgtaa aaaatactga
    11341 gggtgattta tcaaaagaga tgttgagttc atcagttaga gtatttaagg ctatttatga
    11401 tgaaaagtat ggtggttttg gtaatgctcc taaatttcca agtcctcaga atcttatgtt
    11461 tttaatgaaa tattatagta tagaaaaaga caaagatgtg ttaaaaatgg tggaaaaaac
    11521 tttagatgga atgtatagag gaggcttgtt tgatcatatt ggatttgggt tttcaagata
    11581 ctctactgat aaaaagtggt tggctcctca ttttgaaaaa atgctatatg ataatgctat
    11641 gcttactata gcttttttag atgcgtataa aataacaaag aaagagttat ataaagaaat
    11701 tgctataaaa actatagatt atgtagttag agagatgaag gataaagaag gtgggtttta
    11761 ttcagcacag gatgctgata gtgaaggtga agaaggtaaa ttttatacat ttaatcctct
    11821 tgagataatt gaagttttgg gtgaagaaga tggaattttc tttaataatt attttgatat
    11881 aactagtagt ggaaattttg aaggcaaaag tattcctaat cttataaaaa ataaagaata
    11941 tgagagacat aatgaaaaga tagctgacct tagtaaaaag gtatttgaat atagaaaaga
    12001 aagaacatct ttgcataaag atgacaagat acttacatca tggaatgcat tgatgatagt
    12061 tgctttaaca aaggcatata gtactttaaa aaatgacata tatttagagt attcaaataa
    12121 gtgtcttaac tttataaata ataatcttgt aaatgagtca ggtagattat tagctagata
    12181 tagagatgga agttccgact atttagcata tttagatgat tatgcttttt taatctgggc
    12241 gtatatagag ctttatgagt caacatttaa tatgaaatat ttagaaaagg ctttaaattt
    12301 gaatgaaagc tgtatcaacc ttttttggga ttatgaaaaa agtggatttt atatatatgg
    12361 taaagatagt gaaaatttaa tagcaagacc aaaagatttg tatgatggag ctattccatc
    12421 aggaaattct gttcaattat acaatcttat aagacttgcg aaaattactg gagataatag
    12481 acttgaagaa atgtcatata aacaattaaa actgtatgtg gataatgtaa agagttctcc
    12541 aactggctac agcttttata tgctttcatt gatgtttgaa ctttattcta ctaaggaaat
    12601 tatatgtatt tttaaggaag attctgattt aattgcattt aaagaattga taagtgaaaa
    12661 ttttatacca aatgcaactt ttttagcaaa gaaatacaat gaagaaaaca ctattatagg
    12721 ttttttaaat aactatatat tgaaggatga taagactagt tattacgtgt gtcaaagtaa
    12781 tagttgtagt caaccaatta atgatttaca aaaattaaaa gatatgattt taggagtaga
    12841 ataatcataa tagcgaaaaa ggagatgctt taaaataaaa gtctaatcat ctcctttttt
    12901 tgtataaaaa acagaaaaag agttcctaag aacacctaat ctatgtaatg attgttttgt
    12961 taaaaaataa aaatgacatt atagttatat aaaatattgt actataaaaa agtggtatta
    13021 tagatgcgat agtattaatc ttttttattt accctatttc ttctttttgc aaattcttca
    13081 agtgcttcag atttaatttt atctatttct tcatattcca taaaattagc atcttcttca
    13141 aagccatctt gttcttcacc attaaaacga tacatttttt ttagtaaact ccataaggtc
    13201 tctaattcat ctttggtaaa ttcgtgaaat acatctgcca taaagtttat tccagtccta
    13261 ctacatgtta ccataacctt ttttcctaag tctgtaactt taacattaat tgctctctta
    13321 tcatgaggac ttggaattac atcaacatat ccattttttt caagattagc aactagtcta
    13381 tttatatttt gtttacttgt acccattttt cttgcaatat tatttaatgt tgtttcttcc
    13441 tctggtaaat gaagaattga taaaatagtc atatactgtc ttgaagtaag tataccaaaa
    13501 tatttatctc cttctatttg tattttgttt gtaagggtaa aaagagttgc atatgtttga
    13561 ttcattaaaa agagctcttt tagttcattt gaataatcca tgaaatatat ctcctttatt
    13621 tttagatgtt gttttgtcga aaacagaata tcatgaatta attctaatgt caatatggtg
    13681 tcaaaatata ttaaagtatt acttttttat gtaactatta attaattaaa ttattgtata
    13741 attttatgta aaaacttcta tgtaccaatg ataatttcta tgaggaatta ataattctta
    13801 aattttttag aaagattttg acttgaaaat acttgtaata taatatataa attaatatac
    13861 tgaataaaat caatctggag ggataattat gaagtgtaca aactttactt tcaaaggaga
    13921 ggaaggttta gatatatata catataaatg ggaagatgaa aatataaaaa aaccaaaggc
    13981 agttatccaa attgcacatg gaatggctga aactgcacaa aggtatgaga cttttgccaa
    14041 ggtacttact aaaaatggtt atatagtata tatcaatgac catagaggtc atggaaaaac
    14101 tgcaaagata atagaaaatg ttggacattt agctgaaaaa gaaggattta gatgtcttgt
    14161 agaggacatg tatactttaa caaatattat aaagaaagaa aatgaagatt tgccaattta
    14221 tctatttgga cacagcatgg gttcatttgc aagtcaaaga tatataatgg attacagtaa
    14281 taatttatct ggtcttatac tatgtggttc aaatggaaag caaggaatca ttctaaatct
    14341 tgctcatctg ataatcaatc gtgagattaa aaagtatgga agacgttcta aaagtaataa
    14401 aataaataat ttgatatttg gtggcgaaat aataagaaga aatgaaaaga ctaagtttga
    14461 ctggttaagt agagataaag aacaagttga aaaatatata aatgacccat tttgtggagt
    14521 agtgtgtagt tgtggctttt tttatgattt agttcaaggg ttaaaagaaa ttgaggataa
    14581 agaaaactta aaaaaagtgc cacttgatat acctatttac ataatatctg gagataaaga
    14641 ccccatagga aaaaatggta aaggagtctt aagactaagg gatagatata taaaattagg
    14701 agtaaaagat gtaacttgca aactttacaa agatggaagg catgaattgt tgaatgaaat
    14761 aaatagagaa gaagtttttg aggatataat ttgttggtta aataataaaa tagaaatatt
    14821 gtaataaaaa aatataatga tatatttgct ttaaatttat ttatatataa tatttgaaaa
    14881 gtggattatg tatatgcaaa gtagaaaaga gagagcctag aagtgatttt ttgctataat
    14941 gccatttatt gaataggtgg gtgttattat aaataaatta ttaacactca gtatgattat
    15001 cattgtatat ggaatttatt ttatttaaaa ttccttgaaa gtttaaatat agtaagttgt
    15061 aataaagaga aaatctaaat atttaaatgc tatttaatgt gaatctatta attaggttat
    15121 acttaaactt aattaataga ttttatattt caagccaatg agatagggtt ttagattaaa
    15181 atataaccaa tggttaaatt acagacattt tgttaacttt ccctcgattt actagtaaat
    15241 atttttttta tatacttaaa ttacaacaga tgtactaggg aggtatatat gcaaattaaa
    15301 ataggtaaag tcatacagtg tcttagaaaa gaacgaaatt taactcaaga acaattagca
    15361 aagtttatag gtgtgtcaac accagcagta tctaagtggg agagtggaaa ttcttaccca
    15421 gatatagaat tattgccttt attagcagac ttttttaatg tgtctattga taagctttta
    15481 aattacaaga ttgatttaag tgaagaagaa gtaatgaaaa tttataaaga attagaatca
    15541 ggctttgcta gaattgaaat tgatttatca acagaagaac caaaagaaga atttagacaa
    15601 gacttagaat ctgtaaaaaa gctttccaat atgtatatag agaagtatcc taaaagctat
    15661 ttattgaagc taaggatatg ttcgctgtat cagatgtatt catataaatt tggtaagagt
    15721 gaacttaatg acagagtaaa agaaacaatg aatattcttg aagatatagt gagaaataca
    15781 gatgatatcc aaataaaaga aacagctctt attatattat caaacgcata ttgtatgttg
    15841 gaggattatg aaaaggcaga gttgtattta aatatgatac ataagtcaat aggggataca
    15901 agtgttaatt tagctatgat atatttaaag caaaatagac ttgaagaagc agaaatattg
    15961 cttcaaaata aattgtttag taatgtattt aatataagca tggattgtaa gggaataatt
    16021 aatgtataca aaaatcaata caaggaatta aagaaaaaat tggagaatag aaattttaat
    16081 aaaaatgcaa ttgagagaga aatggagtat ataaaaaata agttgctggg gtatgcaaat
    16141 ctttctttag aaataaaaaa aatgctcagt gaagataaag gtgcattttt tagtatgtat
    16201 atggattata tggaattatc tctaattttt ttatttttta acatgaaaga agaagcaaaa
    16261 aaagccttat atagtctaaa agaaatatta gaaaaatatc ctatacatga aagtttggat
    16321 gtaagtcaaa tgagattttt tgataaggta gagtctaaaa atttatatac ttttaatata
    16381 tatacaaact tgttaattgt acttaatgat gatagttata atgagcttag agaagaacca
    16441 atttttaagg atgtaattga aaagatttta gatatggaaa aaatgttgaa aaataaagaa
    16501 taagtaaaaa tttaatatag gacttaaaga ttaatcaaat taaaagaaag tgtttcaaaa
    16561 taaaaatttg aatcaccttt tttgtcggtc ttaaacagtt atttagaaaa atagaggttt
    16621 caaaatagaa atctgaatta tctgtattat tacacaaaaa tagaaaccta aatttataaa
    16681 aatagaataa agtttgttat ataaaatgta attttgtcta taaaatctca cttgttttct
    16741 taactttaag cgtgaaaaag agaatgtatc atgaattttt tagttcatga tgcatccttc
    16801 tttgtatgaa atccaatata tctttattat ttaaacaatg gaaaagagcc ttatttctat
    16861 tttgagacat ctcttttatt taattatgaa gttttataca gttcaataaa taggaattat
    16921 atttagaaac tcatgacatt atttcttaga tagtgattaa atttatcata ttccatttta
    16981 gataactttt ctgggtaaga cattacaatc aggttatgcc cattaaattt ttcatggtat
    17041 ggtggatgaa tgtgttcata attatttgtc ttatatttta ctctttcata gaacgccttg
    17101 cgataaattg aaatctcatc aattggaagg tcaatttcta aaataatagt ttttcctttc
    17161 ttatttaaaa gctctaacgc tctttgtcca tatctattat tgcgcatttc tggaaaaatg
    17221 cagaaatgtt ctacatagat aaagtcatct gtttcccaac ataaaattat gccgacaaac
    17281 tggtttttat cataaattaa attgaactga tattctttat ttttcaaaat ttcttcttgc
    17341 aaagaagatt ttctttgttc atgaaatggg aaactaattt tatataattc catagccttg
    17401 taacataatt tattttcact ggttcttaac cgtttaaatt tcactttact cctcctaatc
    17461 acgaaaattc caatttattt atgagttcat tttaccatag tcagtatttg catacaatgt
    17521 gttttaagtt gagtgtcatt tctttctttt atttatgctt atatctcata gactaagaga
    17581 taattgctta agacataact aaaaactatt atattaagct ttgataatct aaaaacagta
    17641 tatttatgga cttttctagg tctaaaagta atcttaataa ttttagaagt tacaaaaaag
    17701 ttacataaat ttggaaagct ataatataca tatataatat aaggttaaaa ctgtaatgaa
    17761 taatattaat ttaaaatttg aacatggaag gagattatta tggagaaaag agaaaagagg
    17821 ataataatat catctttatt aagtgtatca attttaatgg gtttggtaag tatatattct
    17881 atattaaata aagaagacat aattttaact gttaagggtc aagagcaaaa agtatcttcc
    17941 tttaagaaaa cagttgagga acttttggat gaacagggta taaagtataa ttctgaagat
    18001 aagattaatc caagtttaga tacagaacta aaagatgata tgaaaataaa agttgttaag
    18061 gtaactaaaa gtaaaaaaga agagattgaa aaaattccat ttgatacaaa gcatgtaaat
    18121 gatagtaatt tgttaaaagg aaaatctaaa gtttatcaag aaggtcaaga aggagaaaaa
    18181 aaactagtct ataatttaac ttaccatgat ggaaagttag tcaaaaaagt cttatcaaaa
    18241 gaagtaatat ctaaggagcc aactacaaaa attataaaat atggaactaa agaaaaagta
    18301 ctaatagcat caagaggagc aaatataaga ggaggcaaac atatgaaagt ggttgccact
    18361 gcatatgcag gagatacaat aacatctact ggtacaactc caagatgggg tgttattgca
    18421 gttgacccac gtgtaatacc atatggaaca aaagtatata tacctaaact gggtatgact
    18481 tttgtagcag aagattgtgg aggtgcaatt aaaggtaata gaatagacat ttttatgaac
    18541 agtgaaggaa aagcttcaaa ttggggaagg aaaagtatag atatatatct acattaaatt
    18601 agtgaataga ataactttac ttgaaggaca aaaaagtata aaatatgaat taactaaact
    18661 gttagattat tcttaaaaca taaaaatata tctaaggggg gataagatga aaatagtatg
    18721 cttaggagac agtttaacgt atggatttgg agtatcacga agtaattcat ggacaaacat
    18781 tgtaaataaa gagactcgat tagaaatagt aaataagggt ataaatggtg atacaacaag
    18841 tggtatgtta gctaggttta atgaagatgt tgtaaaaaat tctcctgata tagtttttat
    18901 aatgggtggc accaatgatt ttattgctgg tgcgggaaat gaaattatta attctaatat
    18961 aatggctatg gtgcatcagt catattcaaa aaatattata cctataatag gtataccatt
    19021 aaaaccagat attccaaatg ttagggaaga ttggagttgt tttactgatt ttaatatagt
    19081 atctcaaaac ttagaatcat atagttattg gataaaaaaa ttttgtatga catttaatac
    19141 taatttcgta gatttttatt cagaatataa taaaaatatg gaactagaag gatataaaaa
    19201 attatatttt gatgggttac atccaaccaa agagggtcat agaataatgg cagacatttt
    19261 tattaattca attaataagt atatagaaga gttatagatt acattaaaat gcttgtttat
    19321 gcgtcagttg caataaatac atacagtaga gaggggttta ttatggatta tactaaaact
    19381 tatgaagagt ggataaaggg ttcatatttt gatgaagata caaagctaga gttggagaat
    19441 ataaaaaata atgaaaaaga aatagaggat agattctata aggatttaga atttgggact
    19501 gcgggtctta gaggaataat tgaagcagga acgaatagaa taaataagta cacagttaga
    19561 agagctactt ttggattggc aaactatata ttagaaaaca caacaaaaga agaaacaagt
    19621 agaggagtag taatagctca tgataataga cacaagtcta gacaattctg tatagaatct
    19681 gcaaatactt tggcagcatg tggtataaaa gcatatatat ttgatagttt gagaactact
    19741 ccagaattat cttttgctgt tagaagctta aatgcaatcg ctggtatagt cataacagca
    19801 agccataatc ctccagaata taatggatat aaggtatact gggaagatgg agcacaagtt
    19861 atgccagaga ttgcaaatgc aataacagag aaagtaaata gtatacatga ttatagtaca
    19921 ataccaacct taacggaagc taataaaaat ttggtagttt tgttggacga atcacaagat
    19981 actaaattta tagaagcagt gaaaagccaa attataagaa aagatttagt taaaaatgta
    20041 ggtaaaagct ttaagatagt ttatacacct ctttgtggaa cagggaatgt tccaataaga
    20101 agagcattga aagaagttgg ttttgaaaat ataattgtag ttccagaaga agaaaatcca
    20161 gacccaaatt ttgcagggct tgattatcca aatccagaag agaaaaaagc tttaaataga
    20221 ggtatattac tcgcaaaaga aaaaggagca gaccttgtaa tagcaactga cccagattgt
    20281 gatagagtag gtgtagcagt aaaaactact acaggtgagt atgctttact tacaggaaat
    20341 caaattggtg gtatgctgac acattatata atagaaggat taaaagaaaa taataaatta
    20401 aaagaaaatc ctacaataat aaaaaccata gttacatcag aatttggagc agatatagct
    20461 aaagcaaata atgttgatgt attaaatgtt cttacaggtt ttaaatttat tggtgaaaaa
    20521 ataaaattat ttgaacaaaa taaaaataga agttatgtat ttggatatga agaaagttat
    20581 ggttacttag ttgggactca tgctagagat aaagatggtg tagtttcttc attgttaata
    20641 tctgaaatgg cagcatttta ctattcaaaa ggtatgagtt tatatgatgg tttaatagaa
    20701 ctttataaaa agtatggatt tttcaaagaa cagactatat ctttgacttt aaaaggtata
    20761 gaaggtgtag aaaagataaa agaaataata tcttatttta gagaaaatca aattgattat
    20821 ataaatagta ttaaagtagt tgataaaaaa gattataaaa atggtataga taatcttcct
    20881 aaatctgatg ttttaaaata ctttttggaa gatgaatctt gggtagctat aagaccatct
    20941 ggaacagaac caaaacttaa attttacata gctgtaaaag gagctagtga tattgaagca
    21001 gataaaaaga ttcaaggttt gaaaaagtat atagatgaca tggtcgaaaa attaaaataa
    21061 aatattatta ttttataaga taattttgta ataatattta tttagctgaa aattataatt
    21121 tactttcatt gagtagaata tggaaatgtt agtagaattg ttattttcag cttttatttg
    21181 tgttattttt acttaaataa ttatattata gaatgaatct gagtataatt aatattgtat
    21241 aatgaaatta atttgataga aaaggggata tttatgtttg gtatagataa gaaattgatt
    21301 gaatatataa ttgcaaaatc caattacagt gaattaagtt caaagtcagc agaagtatct
    21361 ttcttcttaa tgttgtccat atttcctttt ttaatattta caataagttc tatagcttat
    21421 ataccgattc ttcatttaaa taagtatata gctttgttta gaaatatgat gccagaaggt
    21481 gcttttgctg tgttatcttc aatcatagtt tctgcaatag acaatagaaa cctgaaattt
    21541 ttagcagtca gttttgtact tactatgtgg acattttcaa gagcagtaaa agctcttatt
    21601 aaaggaatga atagagccta taaagttaaa gaaactaggt ctttttttaa aattttatct
    21661 atatcatttt tatttacaat aatgcttttg gtccttattt ttttatctat gatattttta
    21721 gtttacggtg aaaaaatagg ttattttatt tttaatcttg taggattgga tgagatattt
    21781 ataaaaatat gggatattct aagatacact gtaggtataa taacaataat agtgatattt
    21841 acacttttat ataaatacac tccaaataaa aagttaacta ttaaagaatc tgcgccaggt
    21901 gctatttttg ctacttttgc ttggttttta gtatcatttt tatattctta ttatacaaat
    21961 tactatgcta actatgaagt aatctatgga agtatagctg aaataattgt acttatgacc
    22021 tggatgtatt ttagtagttg gtctatcgtt attggctatg aggtaaattc aagattgtat
    22081 tttagaaaga taagacatga aatgttaaag taaagtattt aaaataaaag agagtattta
    22141 gtattataaa tgtaattgaa ttaataaaga aatattgaca tttaagttaa aaagtctaga
    22201 atttttctgg gcttttttta tttttagtat aacttgacaa attttattta atgggatagt
    22261 tttaatttat aatcatcttt aaaaagatga ctattgataa gtaagagata aaattggtaa
    22321 gtatcaaaaa atatactaaa taaaaattat agtcctaatg agttaatttc tgtattttca
    22381 aataatgcag tatttagtca tgtgttttta tatgcgcctg tatttctaat tagcaatata
    22441 ttagaataag ctactgtaat tagatttaga aaaaagcgac ataaataatt ataaacaaat
    22501 aataaattac ataaaattaa atttattcac aagtatatct tataaaatat gttataatta
    22561 atagaaaatt gtcatataat taattaagta gtattattat aaataacatt gaaaggtagt
    22621 ggttagaata tggataccca taataaatat gtaaatttta taaaaaatat acctgtacct
    22681 tttttatatt gtagaattgt aaagagacaa gaagatatag aatatcgagt agaatatata
    22741 agtaagggta tgggcaaagt attacaatta gaagaaggta tttgtgataa aaatatacta
    22801 gatgtattgc ctgtatttaa gtctaaaaaa tatttcaaag agttgttctc aaatgaagtg
    22861 gattgtataa aaaggtacat tccgacactt aaaaactgga taaatataaa aaaacagata
    22921 ataggggatt catacataat tttgtatttt ggtaaaattg tatttgatta tagacagata
    22981 atcgattcct ttgataaaaa ggaaaaagta gcttatatta aagacgaaga aggcatatat
    23041 atagattgta gtgaaaattt aataccaata ttaaataaca acattaaaac aacgaaagat
    23101 atctttggta aaaatgatat agaagtatgg ggagagaata caggaaaatt atttagagat
    23161 gattatagag aaggagtatc tagtaaaaaa agatttttac aaaatctttt tgaatatgaa
    23221 gaaacatttt ttatggttga aaaatatttt ttgtatgatg aagacgaact tttaggtact
    23281 atagggatag tagacaatat aatatatagt ggttacagta atagaaatta taactcaaaa
    23341 gatttaatga agatgataga acactcaatt ccagagaata tgttttataa agatgtgtat
    23401 ggaaattata ttggatttaa ttcaggtttt ttgaatttag cttgtatgaa taaagaagaa
    23461 ttattaggga agaatagtta taaaatatca gaagaagaag ctttaataga taagatattt
    23521 gaaagtgata agggtgtagt tgaaaataaa aaagtagtta catttgagtt aaatataagt
    23581 atgaatgatg aaaacaagtg tattgagatt acaaaaaggc cgttttttga tagctatgga
    23641 agtgttattg gaataattgg aacagcaagg gatataagta gaagaaaaag attagaagag
    23701 gaaatggata aaactaggat ggaatttttt gcaaatttat cccatgaact tagaacgcct
    23761 ataaatttaa tatcatcttc attacaagtg atagaaaaaa aagaagcaga tttaatagaa
    23821 tcaaatgata ctttaaaaag aaatctaggt ataataaaac aaaatggaaa tagaatattg
    23881 aggttagtaa ataatgttat agactttaca aaaatgcaat caggatattt agattttaaa
    23941 ccagaagaat ctgatataat tgcatttata gaggagatat gtatgtctgt agctgatttt
    24001 gcaagccaaa ataatataca acttacattt gatacagaaa tagaagaatt ttcaatgtta
    24061 tttgattcag aaaaattgga aagaataata ttaaatttac tttctaatgg aattaagtac
    24121 aataagaaag atggaaaaat aaatatattt ttatatgtga aagataatgt atttaatatg
    24181 aaaatatctg atagtggtat aggtatacca aaagaaaaaa tagataaaat atttaatagg
    24241 tttgaacaga ttgacaatga actttcttac agagttaaag gaagtggtat tggactttca
    24301 ttagtgaaat ctttggttga gttgcatgag ggaagtatat ctttaaaaag tcaacttggt
    24361 attggaagtg aatttatagt ttcattgcct gttagaagta aaaataatat tgaaaaatat
    24421 aatcataaaa gagaaatcag caatgaacta agtaaaaaat tagaaataga gttctcggat
    24481 ttataagttt aaatataaaa aataaaattt taaaaaagta ttgacaattg atttagaagg
    24541 gtttaaaata aattacaaca taataaataa aaaggcgatg aataggaaga gtaaatctaa
    24601 ttcgattacc agagagaaga gtccgttggc tggaagactt tttaaattag agggattgaa
    24661 aactacctag gagctacttt ctgaagtcat ctgcattagt agattttagt aagaatagtc
    24721 gttagctgcg ttaaagtgta aagtgagttt tcatgacata tgaaaactaa attaggtgga
    24781 accgcgggaa taatactctc gtccttatgt tataaggatg agagtttttt gttatttaaa
    24841 aatataaaca gtaatcataa taattataga aaatataaaa gcaatgaata ggaagagtaa
    24901 atttaattcg actatcagag aggaaagttc acaggctgaa agactttcta agtttgagaa
    24961 attgaaaact acctaggagc tgtggtctga agttacttta aaaagtttta gtaagactaa
    25021 ccgttaactg cgttaaagtg taaagtggat tttatcaata aatgataaaa tcaaattggg
    25081 tggaaccgcg agtatagact cgtcccttta tatagggatg agtctatttt tattgcaata
    25141 aaagattgaa aataaaaaaa aatcggaaaa ttataaattt aggaggaaaa taaaatggag
    25201 aataaagtga aaattggagt actaggatat ggagttgtag gaagtggact tatagatata
    25261 atagataata ataaagaaaa aagaaatata gaaatagttg gaattttagt taataatttg
    25321 gaaaaacata aggataaaaa atattctaat ataataacta ataatataga tgatatattt
    25381 aataaagata tagatatact tgttgaagtc atgggtggtt tagaaccatc attatcttat
    25441 ataaaaaaag cattaaacaa taaaattcat gttgttacag caaataaaga tttgttagct
    25501 gaatgtggag atgagcttgc aaaattagca agtgaaaata aagtaagtat taaatttgaa
    25561 gcttcagttg caggaggtat accagtatta aaaccaataa tagaatcact agaaggaaat
    25621 aatatagata gtataaatgc aattttaaat ggaactacta attttatatt atctaaaatg
    25681 tatgatgaga atctatctta tgatatggca ttaagacaag ctcaagaatt aggttttgct
    25741 gaagcaaatc cagagtcaga tgtattaggg tatgatgctg ctagaaaact atcaatatta
    25801 tcaactctag cttatgacaa tagagtatat tggaaggatg tgtatttaga aggaattaca
    25861 gatatagatg aaaaagatat tgaatatgca aaaaaattaa attgtaaaat aaaattgatt
    25921 ggtcaaagta agtatgaaaa tgataaagta agtgcttttg ttagacctgt tttagtagaa
    25981 aaagataata tccttgctag aatagacaat gagttcaatg ctgtcatagt gaatggagat
    26041 tcagtaggag aagtttcttt tgtaggtaaa ggcgctggaa gtttagcaac aggaagtgcg
    26101 gtttattcag atgttataga tataatagat aatagagttt caagtataga ttcttttact
    26161 aaagataaaa tacaagttaa taaaatagtt agagaaaagt gtggagcact tcttagattt
    26221 aaaaaatgta ataaagatga aattctaaat atagttgaga attgtttagt aaaattcgat
    26281 attttaaatg atgatgatga acttgctatt atggtttatg ctgattctga atatgaaata
    26341 aacaacagtt tatgtctaat aaaagataaa ggatactgcg aaaaaatgaa taaaatgttg
    26401 aaaataagtt aaaaaagaaa aaatataagt ttatatgtaa catattttga cgattttaat
    26461 actatatagt atgagatgaa acaaaacatc tcataaaaaa attaaaattc aagggagaga
    26521 tatgttatat ggaaaataaa aaatatgcaa atggtggtta ttcagaagac tggtatgaaa
    26581 gaggagaatc tacagctaaa tggttccaga atgatagaga agaatatgaa agagcagcat
    26641 acgatgaaga tagagaaaga agaggctcaa actgtggatg ttcagattca ggagaaaata
    26701 gacctagaaa ctgtgaaaga tttagaagag aagcagagat aagagaaaga gaagcaagag
    26761 aagcattctg tgaatcttca gagagaaaga aagaagcact agcatatgaa tgtgaagcta
    26821 gaaaattatg ggaagaagca gaaaaatact gggatgaata ttcaaaatac aactataaag
    26881 gaatcgaata tttagcagaa gctgctagat tatttgatga aggtatggaa tgtgaagcta
    26941 gaagaaatgg aaataatgga ggaaacaata ataattgttg ccataaatgc cataaatgta
    27001 attgtaactg ctgtagaaaa taaaaaaatt aattgacaaa tcaatagtta aaatatatca
    27061 tattatgtat aataaagaga aaattcagaa aaaaataatg attagaagaa atataagtat
    27121 atttattaga caaatagtgc tcagttagtg aggtgagtac aatatgatta aactcccatc
    27181 ctctagcttt tgatagaggg tgggagtttt tatttattta tatatcttaa atgaggataa
    27241 tattgtaaaa taaatgagta tgagattgta aaattaggag gtaaaaaatg aagagtatta
    27301 aaacatcaag tatgaaagaa aaggtagatg aaaagatatt aagagagcaa gtaagttcta
    27361 taatagaaga tataagaaat aataaggatg ttgcacttaa aaaatataat gagaagtttg
    27421 atagaaatac aagagatgaa tttagaatta ctaaggaaga aataaaggaa gcttataagc
    27481 atgtagatga cgaatttata aataacttaa agattgctgc caaaaatata agagaatttg
    27541 ctaaagctca aaaactaagt tttgaaaacc cttttgaaaa agaaatttat ccaggtgtaa
    27601 tcttaggtca aactaatatt ccaatagagt catgtcttgc atatgttcca ggaggtcagt
    27661 atccattgtt ttcaacagct cttatgctta taatacctgc aaaagtagca ggagtaaaaa
    27721 gaatagtggc atgttcacca actatgaaaa atactgaaaa gataaatcct aagacattgg
    27781 tagctatgga tatagcaggt gctgatgaaa tatatgcaac tggaggagtt caagctatag
    27841 cagcatttac atatggaaca gaaaagatta atccagttga cataattgta ggaccaggaa
    27901 ataagtttgt aactgaagct aaaagacaat gttatggaca agtagggatt gattttgtcg
    27961 ctgggccaag tgaagttctt ataatagcgg atgaaacttc aaatccagtt tatattgcag
    28021 ctgatttatt agcacaatgt gagcatgatt taaatgcaag aggaatatta ttgacaaata
    28081 gcttggaagt agcacaaaaa gttgaaaaaa atatagaaag tatgctaaaa gatttgccaa
    28141 caaaggacat agcatatagt tcatgggaaa ataatggaga gataatattg gttgatgata
    28201 tggaagaagc cattaaaata agtaattttt atgcaccaga acatttagaa gttgcagtaa
    28261 atgagtataa tgatatttgt gatagattaa ctaactatgg ttctttattt attggaaatc
    28321 tttctgctga ggtgtttggt gactatgttt caggtaccaa tcatacatta ccaacactta
    28381 aagcatcaag atatactggt ggtgtttggg ttggaacttt tatcaaaact tgtacaaagc
    28441 agatatttaa tgaaaaagct atacaatcat tagcacctgt agctgaaaag ctggcaaaag
    28501 aagagggatt atatgcacat gcaaaggctg ctgaggttag gtttaaaaag tagtaaaaaa
    28561 ttttattttg gcagtcgaga tttagtgtta gactgagata aggaaaacgc tgagaacccg
    28621 aataaatact atattttgtg ggcctcggcg tttcttttat atctgtatat aataggataa
    28681 tactacctgt gtgtttaaat gctagcttaa tatttctttt ctaaataatg tttatgaaat
    28741 agttaatatt tttagttaat acttgaaaaa aacttttgct aactgtgcaa attcatctgc
    28801 attcgtcaat atagcagggt gtcctccctg actaaaaatg tgaatacttg cttttgaaat
    28861 aaaagttgcc atttgtttgt actcttcttc aagattatga cgacacattt cgtcttcctt
    28921 acttcctgta aataaaattg gcatttctaa tttacacaat tctttatgaa acaaagggcg
    28981 tttctcgttt gcacattgca atagagcttt tgtatcaaga ttaacaacat tctcccaatc
    29041 ttttccatga caccattcat agaattttcg tgcttgaata tcttgctttg atttttccct
    29101 tccagtgatt agattatcta tgaagttatc attaagagta cgtccatcaa agctatctgc
    29161 tataattgct tcaactaaat cgggacgttc taatccagta ttaacagctg cccatgctcc
    29221 tccacttgtt ccaattaagc caactttacc acaattcaaa tgttcaatca atgctattgt
    29281 ctgtaaggct tcatcatgcc atatgtcagg agaaaattga gaaactctat ccgactgtcc
    29341 attacctaaa aaatcaatta agatacatct aaaattttct acatataatg gcacaagtgc
    29401 tttaaacata ttagaagaac cagtattacc atgcaggaaa attatcggtt taccttgtcc
    29461 atattcttta taaaaacaag aattaccttg atactgaaaa tatgacatat attttcctcc
    29521 tttttatttg ttaagttaat tataacaata taggtatgaa tgaaaaacct catgttcttt
    29581 cttagaataa aaatgtgcca cgcataaaac gtgacacatt tctaattata aattttgctg
    29641 tttaagtatt ctttgtatgc ttttctctgt caaaaagtat ttctctgata actgcttgat
    29701 actcattcca ttttggaaat ttatacagat ttgagaatta cgagaagcta agacttgttt
    29761 ggtgtttgtg ttttcacccc aatgcttttt gtgctcttgc tttttgggaa tataaatcac
    29821 tttaccatct atatattctt gcacttgctc aatcagttct ttaggcagta agtcaatcga
    29881 attttgatag ctcattttgc acctcctaaa taattattct ttaggattag caaaggctac
    29941 tgccactcac aattaaaaat aaaatttttt attatgtaca atagcctttg ctatgcacaa
    30001 gttccaattc ccataaacta aatactcctt tctggtttga aaaattcatc tgaacattga
    30061 tttcgctttt aagatagagt tcaatgacaa atttacattt ttattatata tattcagata
    30121 gataattgca actgtctttg tatgaggata ttttattata gtcaatacta taatatgtat
    30181 ttatatcatc aaactaatat gaacagttga aaggaatatg aacaataaaa cttagtcagg
    30241 aagaataatt atgaagcaga caatagagaa atttgatttt aaagttttat tgaaaattta
    30301 ttgacaaatt taacttgtaa atattatact aaacttaata aataaagttt ataaagtaag
    30361 gtgttgtcta tgaaaaatat agttggaaaa ccgcaaacga taaaaaaagt aaatgaagat
    30421 ttaataaaaa atattataaa aaatgaaggt cctataacaa aaccagaaat ttcaaacatt
    30481 actgaattga gtttagctac agttaataag attgtagagc aattagcttt gaaaaatgaa
    30541 gttaaagtaa gtggactaag tgaatctact ggaggaagaa gagctcaatt atttgaaata
    30601 aatgcaaact tagaacacat aatagcttta tattattata gaaattgttg tataggggta
    30661 gttgccaatt tattaggtga aataatttat caagaagaat ttaatataag aatggataca
    30721 tatgaaaacg ttaaccaaga tacttttttt gttatagata ctctaataaa aaaggaaaaa
    30781 ggaaacaata taagggctat tggaattggt gtcccaggag ttgtaaaaaa aggtgtagtt
    30841 aaaaatatac caactataaa aagttgggaa ggtataaatt tagaaaattt aataaagagt
    30901 aaatacaata ctaaagtgtt tatagaaaat gatacaaata ttactactat tgggatttat
    30961 caaaaacagt ataaagacaa atataaaaat atggctctta tgtatttaga agatggtatt
    31021 ggttcaggta tagttataaa tggtgaattg tatttaggtt ctacaaattt tgctggagag
    31081 ttaagttatc taaatataga atttggtaag tataactcta acaacttaaa tcttgaagat
    31141 tatattatga gtttaagaaa aagatattta gaaagtaaag ataataaatt taagaaagaa
    31201 attttatcta taatatcaaa ctttataagg actcttgtat gtgtcctaaa tccacaagct
    31261 atgattatac agtgtagtat attaaccaag agtgacatgg agtttataga aaaagatgta
    31321 agcaataatg ttggagaaga aaattttcct aaattaataa aagtagagtc attgagagaa
    31381 ggaagtataa atggagtaat tagtatgtgt ttaaaagaaa ctgactatca atattcctta
    31441 tctaataaaa aaggaggata ttaatgtgaa atatttggtt tcaatagatg gagggggaac
    31501 aaaaactaaa ttttgtgtaa gtgacttaga tggaaacata cttaaagaac ataccactgg
    31561 ttcaactaat tataagtcag ttggaattaa aaaaacatat gaaaatatta ataatggatt
    31621 taaaaaaata ttaaaagatt tatatatcga ttatgacgat attgagtaca ctgtatttgg
    31681 tatatctggt tgtgattctc ctaatgatta taagattata atggatgaaa tattaaaaat
    31741 tgggattaac aaagaaaaaa tatatctggc aaatgatgca gttttagctt tttatgcaca
    31801 agcggattcc cctggtttag taattgtggc tgggacaggt tcaattatac tgggaattaa
    31861 agaagatgga gaaatatata gagttggtgg atgggggtat aattttagtg atttgggttc
    31921 tggatatgat atagggagaa aattattaaa gaaagtattg ctttattgtg atgaatgtca
    31981 tgagtatagt gatttattta gttgtgtctt ggatttcttt ggagcaaata gttttgaaca
    32041 acttgcatat atgattactg atataaacaa taatgttgaa atagcaaact tagcttcatt
    32101 agtgattgat tgtgccgaac aaggtgataa attggctatt gaaatattaa gagaatcatc
    32161 aactgaacta tctaaattag ctcaagtaat attgagaaaa atatctaatc caatgaaagt
    32221 ggataaaagc gaaataaata tagttttgtc tggtggtaca ttaaataaaa ctgtatatgc
    32281 tcaaatgtta atgaataatt taaaaacaga aaatacagat aaaaatttaa aatttatatc
    32341 tcaagaaaat cagccagtgt atggggggat aagacttgct atggcattag ctaataggag
    32401 ggtcaagcat ggatagaaaa aaggtaatag tacaaatttt tactggaggt tttaaggcta
    32461 aagaaataaa gtttaatgat ataaaatgta aactactaga attattggac tgtattgata
    32521 tagaaaaggt tatcatggga tggagtgtag atgaagaatt atatgtagaa actaaaaaac
    32581 tattaaataa atataatgtt gaactatatc tttggttgcc atgtttttct gaaataggat
    32641 tacttgaaaa aagtaatttg ctaattgatt atatggggaa ggaagtcaag ggctatgctt
    32701 tacaagagga tgaaaatttt gagttttatt gcccaaataa taaggaaaat ataaaaaata
    32761 taaagaaaat atatagtact tattttagtg atattaaatt tgatggaatt tttttagata
    32821 aaataaggta tggttctttt tcgaataaac taaatggtgt ttttaattgt ttttgtaaag
    32881 attgtttaaa tatatatgaa aaaaataata ttgataaaga gtcgttaatg agagaaatga
    32941 ataatgtgtc taagggagtt atagataatg aagatactcc atttggtata acagaataca
    33001 ttaatggaaa atacaaattt aaaaataaaa tatgggaaga tttttttgaa acaaaaaatc
    33061 aaaatatata caaggcatta ttagagataa gtaattattt taaaagtaag ggattgaaaa
    33121 taggtataga tactttttct ccttttattt cttactttac aggtcaagat ttagaaaaat
    33181 tacaagaagt agcagatttt ataaaaccaa tgatgtatag aactacaaaa gctccagctg
    33241 gtttaccatt tgaatttgaa aggtttattg atgaatccat acataagaat aaagagattg
    33301 gaaaaagaaa tttcttagat attttaggaa ttaaggagta tgaaggagat gtatatccta
    33361 tagaatttgt actagaggaa atcgatttta tgacaagaaa tcttaataaa gatatatatg
    33421 taggagttga aataaataaa aaggaaatta tatctcctgt tacacctaag tacataagtg
    33481 aaaatatttc aaatataaat aagacaacag taaaaggata tgttttatct tgggatttgt
    33541 tgtcagcacc aaaagaaaat attgatgaaa ttataaaatg gtttcggaat tagtgaggtg
    33601 ataaagtgag taagatattg gaaatgcaac aaatatataa aagttttgga aaggtagatg
    33661 ttttaaaagg gataaatttt gagttagaaa agggagaagt aagagctctt ttgggagcta
    33721 atggagcagg aaaatctaca cttattaaaa ttttaggagg agttcattct caaactaaag
    33781 gtaaagtaat tttgactggt aatgaaataa tgtttaaaaa tccacatcat tctaaagaaa
    33841 atggtattag tataatttat caagaattaa gcttgatacc aacgcttaca gtacttgaaa
    33901 atatttttct tggtagagaa attttaaaag gaagtgtgtt tttaaacaag aaagacatgc
    33961 ttgaggaatg tatgaaaatt tgtgaggatt ttgattttaa tatagaccca catatggtag
    34021 tttctaattt aagtgtagca aaccaacaaa tggttgagat aatgaaagct gtttcatgta
    34081 attctgaaat tatcataatg gatgaaccta caacatcact tactaataat gaaaaagatg
    34141 ggctatttaa aataataagt agactaaagt ctaaaggtaa aacaatagtc tatatttctc
    34201 acatattaga ggaagttttt aaagtatgtg atagtgcaag tataatgaaa aatggaatta
    34261 tgattggaac ttatgatgta gaggatttaa ctaaattaaa gataactcaa cttatgacag
    34321 gaaatgataa tcacaaactt attgagaaga aggattatac atttgcaaaa tatgaagagc
    34381 caccagtact agaagtaaag aatataagct ggtctaacaa aataaaaaat gtttcattta
    34441 aagtttatag aggcgaagtt gtaggtcttg caggtcttgt aggttcaaga agaactgaaa
    34501 ttataaattt gatatttggt atagataata aagatagtgg agacattttt atcaatggta
    34561 aaaaagtaga cataacttct cctaaggttg ctataaaaaa caaaatagga ttgattcctg
    34621 aagatagaaa aaatctagga ttaattttag aacaagctat atacacaaac tcatcatcag
    34681 tacaaattga taaattcaag aaattcttgt ttatagatag aaataaagaa attgagtttt
    34741 ctaaaaaagg tgttaaggaa ttaggaataa aagttaatga tgtaaagcaa gaagtaaaag
    34801 agcttagtgg tggaaatcaa caaaaagtag tagtttctaa gtggttagag aaagatttag
    34861 atttaataat atatgatgag ccgacaaagg gaatagatat tgctgcaaaa gaagatattt
    34921 tcaatacaat caaagaattt tcaagtaaag gaataggagt aatatttata tcttctgatt
    34981 tagaagaagt aataagagta agtgatagaa ttttagtaac tagagaaggt tctattgtag
    35041 atgatttaaa aaatgagaat atacaggttc aagatataat gaacagtata tttaatgtat
    35101 aaaaaatgaa aatggaggac tgaaatgaag aaagcttttg agaagttaaa tttaagagat
    35161 tatggagtat tattaggttt tttagcttta tgtatagcta taagtattgc aactccatca
    35221 tttttaggta aacaaaatat acttaatctt ttacgacaat cttctataat tggaataatt
    35281 tcagcaggta tgacttttgt tataatatct ggaaactttg atatatcagt tggagcagta
    35341 gcagctcttg caggtgctat aacaatgaaa tttgcgactg gtggaactaa tttgtttgca
    35401 tccatgctct taggtgtagc gatttgtgca gtaataggat taatcaatgg tatagtagtt
    35461 gcaaagataa atgtaccatc attaatagcc actatggcta tggtcactat tgtaagagga
    35521 ttgctgttga tgcttacagg aggatatcca attactgaaa atataccaat acttgattac
    35581 attggaaatg gatatttgtt taatatacca ataccagtag taattttctt tatagtggtg
    35641 ttagcttcat ttattgtact taataaaact aagtttggta gatatgttta ttctgtagga
    35701 gggaatcaag aagcaagtaa acttaatggt ataaatgtag attctcacaa agtgaaagta
    35761 tttattatta atgctgtact tgcaggaata gctggaatcg tactaacagg aagacttggg
    35821 actgcaactg cgattgcagg tgaaggatat gatatggatg caattgcatc tgtagtaata
    35881 ggaggtacat cagtggcagg aggttctggt tctgtactta aaactgttat aggagtatta
    35941 cttatgagtg ttataaacaa cagttttaat cttcttggaa tagatgtata tttccagtac
    36001 atatttaaag gattaataat tttagcagca gttggatttg attcttatag caagaaaaaa
    36061 cttgcttcaa gataaaaaat aagggggatt tttttatgaa aaaattaatg aaacatttag
    36121 cattgttgct atcacttgtt atgatatttg gtttagttgg ttgttcaaat ggtggtgaca
    36181 aagaaaaaaa tggagaaaag aaaatagcag tattactacc aggttcaaca ggttactttg
    36241 tagcaactaa acaaggtata gatgcaaaat ctaaagaatt aggtgtaagc gtggaatatg
    36301 cagatgctca atgggatgct agtaaacaat tatcacaagc tgaagatttt atggctaaag
    36361 gggtagacat gattgcatta tgtggagttg attctgctgt aagtgagaga atagttaaag
    36421 ctgctaatga ttcagacgtt cctatagtag catttacaaa ttctatagga agtaacccaa
    36481 ctggagaatt taaaggttta gttacatata taggacaaaa tgaggaagaa acaggagcat
    36541 taactggaaa aattgctaaa aacttattag gagaaactgg tgggaaagct gtattaatag
    36601 aaggagtacc aggaactact ccacaagtat taagaaaaaa aggtcttgaa aaagaattaa
    36661 aagatagtaa tatagaaata gtttataatc aaacttcaaa ttgggagaaa gagcaagctt
    36721 taaaggtaac agaagattta attcaaaaga aaactgattt taatgttata atatgtcaag
    36781 atgataattc tgctacaggt gcaggtcaag ctttaaaaga tgctggtctt aaggataaag
    36841 ttaaagtcat aggtttgggt ggaagtaaag atggattaaa agctataact gatgggttaa
    36901 tagatggtac aacttatatg tctgctgtag aagaaggtgg attagtcata gaaaaggcat
    36961 ctaaattcct taaaggtgaa aaaatagaac ctgttacaca aattaaacaa gttgaagtta
    37021 acaaagataa tatttcagag ttcaagggtg aatggtaaat tgtcatagat aataaataag
    37081 atttttagag ttactttaga aactatataa agtaactctt tttttaattt aatttagtga
    37141 taaattattg aatagtattt ctaaacaaat aaaaaattaa tataaacaaa gacttaatta
    37201 tactaaatta taagtaaaac taatataaac ttatggaaaa aaggaaaacg tctaaagtat
    37261 tggaattact atagtgtagg tgtggtatta aaaaaacata aaaaaacata aaaaaattgt
    37321 tgaaaaaaat atagaaattt tgtataatta aagatttgaa aagtgcggta tacaaagcca
    37381 attcttttgt atataatata tataaattca gaaaaaggaa gggatatgta tggccaggcg
    37441 gttattttta gaaaggattg gacttatatt tttaggttca gcaatattag catttggagt
    37501 ttataatttt tattatttaa acaacataac agaaggtgga gtgttaggta ttttactact
    37561 gttaaaaaat ttattcaata tacaaccagc aatagcaaat gttgtaatag atggtttgct
    37621 acttttagta ggatataaat ttttcggtaa aaaattcctt atatattcaa tagtagcttc
    37681 tataactttt tcagtgttat atgacttatt tgaagctata ggacctctag taccacaatc
    37741 acaaaatatg cttttatcaa ctattttggc aggtgtaact gttggaactg gtgtaggtat
    37801 agtagtaaaa gcaggttgtg catcaggagg agacgatgct ttagcattag taatttctaa
    37861 gactacatct ttgaacatag ggcaagttta tttagcaaca gatgttattg tattgttgtt
    37921 atcattattt tatctatcag cgtttgatat cttttattct ttgattgctg ttacaattag
    37981 tggcaaggta attgatttta tttattatca tggtaaaagt ttggatatgg atataagtaa
    38041 tgatatagtt ccagaatgtt aatataaatt aaataacttt agattttatt aggggtggca
    38101 gatttgccac cttttttgtg tctaaatctt tatagaaagt ataatcctat ctaggttttt
    38161 gcaaatctca aatatagaat tatgttgaaa tgactgggaa tacgattgct agatgtgcat
    38221 tttaaaaacc caattttata tttatgaaat actaatcatt aatggatgtt tcttaatcct
    38281 atctttatac ctaaaatgat aagataaatg aagtagcaat acaaaactaa aatttgtaaa
    38341 agagattaaa ttaaatggag gagctaaaat gttggatata gttatgatag cattgttatt
    38401 cataggattt gtttccatga agttacttgc tgattggtgt gggaaacaaa ctgaaaaaaa
    38461 ataatgtaat gtatggaggg attacctatg tggtttctgg cagttgtaat tataatgtta
    38521 ataatatatt tggtatatgc gcttattaat cctgaaaaat tttaaacagt ataaggctaa
    38581 taagtaagtt gaattggggg gaacaatttt tatggtgcag atagtaatag tgttagcgat
    38641 ttttatgatt ttagtaatac ctatgggcaa gtatttatat catatagcta ctaatcaaaa
    38701 aacatttgga gatagactat ttgataaagt ggacaatttt atatataagg tatgtagtat
    38761 agataagaaa aaagaaatga actggaaaca gtatgcatta gctttattat ttacaaatgc
    38821 agttatggta tttataggat atatcatact tagaactcaa agtatgcata tttttaatcc
    38881 aagtggaatt aaaagtatgg aacaaggact atcatttaat acaataataa gttttatgac
    38941 aaatacaaat cttcaacatt attcaggtga atcaggactt tcatacttta gtcagatgac
    39001 agtgataata tatatgatgt ttacatcagc tgcaacagga tatgcggctg ctatggcatt
    39061 tgtaagaggt ctagtcggta aaaagaaaac attgggaaat ttttatgtag atttaattag
    39121 gataacaaca agagtacttt taccaggagc tttaattatt gggcttatat tagttacaca
    39181 aggagtacca caaacttttg ctgggacaga aacagtgact actatagaag gaaagcttca
    39241 agatatagct agaggtcctg tggcagctct tgaatcaata aaacatttag gaacaaatgg
    39301 tggtggattc tttggttcaa attcatcaca cccatttgaa aatccaacaa taatatcaaa
    39361 tatagtagaa atattatcta tgatgatatt accaggagct tgtgttgttg catttggtca
    39421 catgataaag aataaaaaac aaggttgggt tgtgtttggc gcaatgtcaa tcatattttt
    39481 aataggtcta gtagtgtgct ttaaagcaga aagtgcagga aatcctattt tatcacaatt
    39541 agggcttaat caaagtatgg gaagtatgga aggtaaggaa gttagatttg gtatagcaca
    39601 atcatcatta ttcacaacag tgactacttc ctttacgaca ggtacagtta ataatatgca
    39661 tgatacactg actcctttag gaggattagt accactttta aatatgatgc taaatgtagt
    39721 atttggtggt aaaggcgtag gtctgatgaa tatgttgatg tacgctataa tagcagtatt
    39781 cttatgtgga ttaatggtag gaagaacacc agagttttta actaagaaaa tcgaaggaaa
    39841 agaaatgaag cttattgctt tacttattat attacatcca cttttaatac taatgttctc
    39901 tggtctttca gtagcaatac cagcaggact tgaaggaatt tcaaatcctg gattccatgg
    39961 attatcacag gtattgtatg agtttgcatc ttcagcagcc aataatggtt caggatttga
    40021 aggtctagga gataacacta tgttttggaa tataacaaca ggtatagtta tgttctttgg
    40081 aagatatgtg tctattatag tactactagc aatatcaagt ttgttagcat ctaaaaaagc
    40141 tgtaaatgaa agtataggaa cacttagaac ggataatttt acatttacaa tagttttagt
    40201 acttgttgtt ttaattgttg gtgctttaac atttttccca gccttagcgc ttggacctat
    40261 ttcggagcat ctagtgttgt ggcactaaaa aattgggagg gagtaaagtt ttatggaaac
    40321 taaaaaaaca aagtttatta caaaagatat tttcaaaact tccattatag aagcttttaa
    40381 aaaattaaat cctaaatata tgatgaaaaa tccagttatg tttgttgtag aaattggatt
    40441 tatagtaaca ttgatactta caatagtacc tagtttgttt ggtgatgaag gcaataactt
    40501 aagagcttat aatgggattg taacaataat cttatttata acagtccttt ttgcaaattt
    40561 tgctgaagct gtagctgaag gaagaggtaa agctcaagca gatacactta agaaaactag
    40621 aaaagataca gtagcaaggg ttatagatac gttaggaaat gaaaaaatga taagtgcaag
    40681 tgagctaaaa cttggtgata ttgttttagt aaatgctgga gaagttatcc caaatgatgg
    40741 agaagttata gagggtatag cttctgtgga tgagtcagca ataactggag aatctgcacc
    40801 tgtcatgaga gaatcaggtg gagatttcgc atctgttaca ggtggtacta cagttgtaag
    40861 tgactggtta aagataagaa taacaacaaa accaggagaa tcgttcttag ataaaatgat
    40921 agctcttgta gaaggtgctt caagacaaaa aacgccaaat gagattgctt taaatacttt
    40981 gttagtaggg ttaacactta tatttatggt tgttttaata tctctttatc caatggcaaa
    41041 ctatgcaggt gtaaagattc caatttctac tatgatagct ctcttggtat gtttaatacc
    41101 aactactata ggtggattgt tgtcagctat tggtattgca ggtatggata gagtaactag
    41161 atttaatgta attgcaatgt caggaaaagc agtagaagct tgtggagacg ttgatactat
    41221 gattttggat aagacaggta cgattactta tgggaataga cttgcatcag aatttatagc
    41281 agttggaaat gcagattcaa aagatttaat aaattattct gttatgtgtt cattaaaaga
    41341 tgatacacca gaaggaaaat ctatagttga tttaggtatg aaattaggtt caacaggtaa
    41401 gacaaaagag gcagaggaag ctgagtttgt agaattttct gcacaaacta gaatgagtgg
    41461 tattaactta gctgatggta gagctataag aaaaggtgct tatgatagca taataaaaag
    41521 agtaaaagaa tctggtggta cagtgccaga agatttagaa gaaaatgtaa atcgagtagc
    41581 taaactagga gggacacctt tagtagtgtg tgtagataat gagatatatg gaattatcta
    41641 cttaaaagat acagtaaaat cagggcttgt agaaagattt gctagacttc gagagatagg
    41701 aataaagaca ataatgtgta caggggataa tcccttgaca gcagctacaa ttgcaagaga
    41761 agctggagta gatggattta ttgcagaatg taaaccagaa gataaaatag atgcaataaa
    41821 aaaagaacaa ttagagggta aaatagttgc aatgacaggt gatggaacaa atgatgctcc
    41881 tgcacttgct caggcagatg taggtcttgc aatgaatagt ggtacaacat ctgcaaaaga
    41941 agcagcaaat atggtggatt tagattcaga cccaactaag atattggaag ttgttgaaat
    42001 aggaaaacag cttttaataa ctcgtggggc tttgacaaca tttagtatag caaatgatgt
    42061 tgcaaaatat tttgcgatta taccagctat atttactctt gcaatacctc aaatggagat
    42121 attaaatgta atgaaacttt ctacgtcatt tagtgcgata ttatcagctt taatatttaa
    42181 tgctataatt atcccatgtt taatacctct tgcaatgaag ggagtaaaat atagacctat
    42241 gaaatcacaa gctatgttaa tgagaaatat gcttatattt ggtcttggtg gagttatagt
    42301 accattttta ggaataaaat taattgattt attgattaca cctttagtaa tggttcttaa
    42361 tttaggttaa taaatggagg atataaaaat gaaaacatta aagccagcat tacttgtaag
    42421 tattgtattg ttagttgttt gtggacttgt atatccactt gttttgacag gtgtaagcca
    42481 agttgcattt aaagataaag ctaatggaag tatgatagaa gtaaatggtg taaaagtagg
    42541 ttcagaactt attggtcaaa gctttacaga tgcgagattc tttaagggaa gagtatcatc
    42601 agtaaactat aatacttata caaaagaaga tttagtacca gataaagatg gaaacacatc
    42661 ttatggaggt gtatcatcag ggtcttttaa ttatggagct acaaatccag aacttcatga
    42721 tagagtacaa aaagatatag aacagttttt aaaggataat ccaactgtca aaagagagga
    42781 tataccaaca gatttactta cagcatcagg ttcaggactt gaccctaata taagtccagc
    42841 ctctgcaaaa attcagatac cagctatagc aaaagcgtct ggaatatcag aaagtaaatt
    42901 acaaaaaatt gtagaagata atacttccaa aaaacttttt ggagttttag gtgaagatag
    42961 agttaatgta ttaaaggtta atgtagaggt cgcaaaaata ttaggtctta tatagtattt
    43021 tgtggaattt tagcaacaaa atattactca agatttaatt taaaggttaa attttaaaga
    43081 gtgaaaatcg ataatgattt tcactttttt atttgaatat tactaatatt tggaataaag
    43141 tgattgtaag tataaaatcc aaaaaaaggt tgcattatca aaatgataat actataatat
    43201 atttatcgtt atcaatttga taatagattt ggacatatag gaggaaatag tatgttatat
    43261 aataacgcat tagagttgat aggaaacaca cctgtagtaa ggctgaataa tttaggctgt
    43321 actaatgggt gttctaacat atatgtaaaa ttagaaaaag taaatccagc aggaagtata
    43381 aaagatagag ctgtttatga aatgttagag ggtttagagc aaagaggaga attaaaaaag
    43441 ggagatgtac tagttgaagc aactagtgga aatactggta ttgcattatc tatgatagga
    43501 aaattaaaag gatatgaagt agtaatcgta atgcccgaaa caatgagcgt tgagagaaga
    43561 gatttaatga aggcttatgg agcaaatttg gtgcttacag atggtaaagc tggaatggca
    43621 ggttccatag aaaaagcaaa tgaactttta aaagaaaatc caaactataa aagcttaaaa
    43681 caatttgaaa ataaagataa tccaaatgca cattataaaa caacagcagt tgaaattatg
    43741 aatgatgtca aagatttaga tatattcgta tgtggagttg gaacaggtgg tactttaata
    43801 ggaactgcaa gatatttaaa agaacaaaat ccaaatataa gagtaattgc acttgaacca
    43861 aaaaaatctc cagctatatc agaaaacaag actggacctc ataaaataca aggaataggt
    43921 gctaactttg ttcctgaaaa ttatgattct agtgtagttg atgaagtaat attagtagat
    43981 gatgaagatg catttgatac agttaaatta cttgcagcaa aagagggaat tttagtaggg
    44041 atatcttctg gagcaaatat atttggtgct atagagatgg caaaaaagta tccagataaa
    44101 aagatagtta cagtagctcc agatggagtt gataagtata tgtctatggg aatattttaa
    44161 taattttatt tgagggagtg aatactttgt ttaagaaaat aaataaagat attgaatata
    44221 taatgaagaa tgatccagca gctaggtcta agatagaagt ttttttactt tatccatcag
    44281 ttcatgcaat gataatgcat aggatggctc atgctcttta taagaagaag aagcttttta
    44341 cagcaagatt aatatcccag atatccagat ttatgactgg catagaaata catcctggtg
    44401 ctaaaatggg ggaaggtata ttgatagacc atggaatggg tgttgtaatt ggagagacag
    44461 ctgaagtagg taatagggtg acaatatatc aaggagctac tttaggagct acaggaaaag
    44521 atactggaaa gagacatcca actgttggtg atgatgtatt aattggtgct ggaacaaaaa
    44581 tactaggacc tttaaatata ggttcaaatt caaaaattgg tgcaaattct gttgtcgtta
    44641 aggatgtacc taatggtgca acagttgttg gtataccagc aaaaatagta aaaataagaa
    44701 acctagaacc tgtaaaaaag aataaaaaag aagtgtccta tgagtatgat gaattagata
    44761 atgtatatta tatataggat ttatataact ataaaataaa ctttataaaa attattttaa
    44821 aaggtatttt tttcaaattt gaggacataa taaaaaagaa ctgaatgtta cagttttggt
    44881 tcatcaatct ttgataaaca agattttaaa tttgtagcgt ctaggaggat tttattatga
    44941 aagcaaatgt taatcaagat acttgtatag gttgtggatt atgtccatca atatgtccag
    45001 aagtatttga tatgaaggat gatgggaaat cacatgttat agttgatgaa gtaccaagtg
    45061 atgctgaaga atcagcagca gaagctagag aaagctgtcc agttgatgca atagatgtgc
    45121 attaataaaa taaatgccta taaaaattga attttcaatt tttataggca tttgtctttt
    45181 attcagttag tatttcaaca cctgtttctg taactaaaac catacattcc cattgagcag
    45241 ataatttacc atcttttgta taaatagtcc aattattttc ttcatcaaca aaaatttcgt
    45301 gagttccttc atttaccata ggttctattg tgaatatcat cccaggaacc aatagcatct
    45361 cagtcccttt tttagataca taacttacaa aagggtcttc atgaaagctt aatccaactc
    45421 catgaccacc tatttctcta acaacagagt atccattttt ctttgcataa gagttgataa
    45481 catcacctat atctcctaag aatccccatg gtttggcaac ttcaagacct ttttggacac
    45541 attcctttgt aacttgaact aatttttttg cattttcgtt aacattacct atcataaaca
    45601 ttcttgaagc atctgaatag tatccgttaa aaatagtaga aacatctaca tttataatat
    45661 ctccatcttt taagataatg tctttacttg gaataccatg acaaacttca ttatttactg
    45721 atgtacatac acttttagga aatccattat agtctagtgg agcagggata gctccatttt
    45781 gtagtgtaaa gtcatgaacc aacttattta tatcttctgt agacattcca atttcgattt
    45841 tatctgaaat ataatccaaa atttttgtgt ttattacagc actttttttt atttcctcaa
    45901 tttgactttc attttttata atatcatgag ttggtacaat atgacctaac ttagcaaatg
    45961 aatctatttt attatccata tccatatgac attttttata ttttttagta cttccacacc
    46021 aacagctatc atttctattt aacttcataa cttttcatcc ttcttttatt atttatttaa
    46081 attatgggtt acataaacta ttttaaaaca taaccattat aaatattata acataaatac
    46141 taagaaatcc tttatagata gtgatttatt caaagtataa attattatat ggttattgtt
    46201 gatgaaaaga ggatagttaa atgtattatt ggatattagc gcacttaata gtttagttca
    46261 agtttttcaa gaaaatcata tgttagagga caattttcct ttaaagtatt ttttgaatct
    46321 tcatcaagat aataatgtac aaatgattct gcaaaaaatt cttctataaa cttgcttata
    46381 tatgcatgtg caacttgctt agggtcataa agcttatatc tttcctgagc aaatacttcc
    46441 ataaattctt cagaatttga ttttttattt aaaacatttt tatctatagc atgtgcagtt
    46501 tcatgtaatt ctaaattttt tgatgaatgt acatttgcat agctagggtt actataacct
    46561 attttagcca ctatagaatt atttctatat ataccaggta catctttcca agtcttgcct
    46621 gatcttaccc aacatggagg taattgatat tttaaatcac taaattctgg ttcatcagtt
    46681 agatttgaat ttattaattt tatttttata tccttttctt taagtgtctg taaatattta
    46741 atatttattt ttgcaagtct gtttttcata ttttcaactt catctttatc ataattcaag
    46801 tcatcaggaa gtataagcat ttgttcaatt atatctatac tactgtcttt tatattttca
    46861 gattcttcat caaatttata ttgaagagta ttattttcat agatagttat atttgtatta
    46921 ttttctatag aataagagtg aatatcctct aaatctaaaa tattcatttg ttgtaaaata
    46981 tgttttgttt cgccactcca cactactaaa gaaagtgaat taaaattaat tagtggattt
    47041 tcttttgctt ttatatcatt gcttatatct acatcaaata catattcaat tgagtcttct
    47101 atgttaagac tatctgtatt ctttttaata tataaatacg cttgttttaa gtctatataa
    47161 atctcatcta aagattcgtc ataaaattct gataaattat tagtaaagag ttcttcactt
    47221 aaaagttcat ttttatcagt tatactttca attaaaatat taatttcttt tatcatattg
    47281 taaatactta tttttctatt tgctttttca ctagatacat tgcatggcgc aagagcaatg
    47341 caataaaaat ctttttcttt tttataaaag gctttaagtg attttgataa aagtgtaaag
    47401 tctacaccta tctgtaagtt gaccttaagt gagtcatttt gtaataattc agtattattt
    47461 cttttagctg tatttaaatt gttttccata atcattgccc cctttttatt agggtatcat
    47521 aattttttta tatgtgtcaa tttgtttttt tgaaaattat aatttttttg aaaattttat
    47581 tttttataat ttgtgaattt tttattatag aaataaatat gttattatta tgtcaaaatt
    47641 tagcattaga ttcatgtaga gtatgtttta tacatatatt ataattaatt ctttgaagat
    47701 tgcatattca aaatacacac catttttttt aattctataa tataatattt atatatttaa
    47761 tgttaggatg tgatttaagg tgtattttac atatattata agatgtaaag atgattctct
    47821 atacactgga tataccagta atatagttag aagaatgaat gaacataaat taggaattaa
    47881 ttcaaaatac acaagagcaa agggatttga aaaactagaa gtttattttg tgaccaacac
    47941 aaaaagcaat gctatgaagt tagaatatta tataaaaaaa ttaactagaa ataaaaaatt
    48001 gtctataata aaaaacccaa gtatacttat aaatttaatt gataataaag aagattatat
    48061 tattggtaag gaaatagaac aattaacata aatatttata gtaacattat aatgggtgca
    48121 tatggtgtaa aaacttgaca ataaatacta ttgtaatata atgaagttat aaatatagta
    48181 aattaaaata gctaggggag ccaagagctg agaggtatac cgacccttac acctgatctg
    48241 gataatacca gcgtaggaaa gcttagtaaa aattcaagta tgtattattt gtgatatatg
    48301 ctatttctaa gctataccta ttgtaggtat agctttttta attaagttat ttaaattaaa
    48361 ctttaggagg caacaatgag taattataaa ataccaacac ttacaatagc cggctctgat
    48421 tcatctggag gagctgggat tcaggctgat ttgaaaacgt ttagtgcaat tggtacttat
    48481 ggtatgagtg taataacagc tataacagca cagaatacta aaggagtttt tgctgtagag
    48541 gatttaaata agaaaataat aaaaaaacaa atagaggcag tctttgaaga tatacctcca
    48601 agagctgtaa aaataggaat ggtttcaagt cctgagatta tacttgaaat agttgagaat
    48661 ctaaagaaat acaatccaaa atatttggtt gtggacccag taatgatatc taaaagtgga
    48721 tattatctat taaagccaga ggcaaaagaa aacttaatta agtatttgat accacttgca
    48781 tacataatta caccaaatat accagaggca gaagaaataa caggaataaa aatacataat
    48841 gtagatgata tgaagagagt aggagaagaa atactacaac ttggacctaa atttgtactt
    48901 atgaaagggg gtcatttaga tggagaagca gtggatattt tagttggtaa aaatatattt
    48961 aaagtttata aaagtgaaag aattgataaa aaaaatacac atggaacagg gtgcacatta
    49021 tcttctgcaa taacatctta tctagcatta ggatatgaaa taacagaagc agtaaatcta
    49081 agtaaaatct atataacaga agcaataaaa agaagttttg atataggtca tggagtagga
    49141 ccagtacatc atttttataa atttgagtaa taaaaaaata ttggaggtag aatatgtata
    49201 atttaattaa agatgtaaaa aaattaaatc cattggtaat tcattatact aataatgtaa
    49261 ctataaatga ttgtgctaat gtaactttag ctgtaggagc aagtcctctt atgagttttt
    49321 catatgagga agtagaagaa atggtaagtg tagcaaactc tgtagtaata aatataggaa
    49381 ctatgaactc aaatatgttg gatttattcc tattagctgg aaaagcagca aataagtaca
    49441 ataaacctgt tgttttggac ccagttggag tatttgcaag caaagcgaga gcagaactta
    49501 caagtagact tttaaatgaa gtcaaattta gtgttgttaa agggaatgta tcagaaataa
    49561 agtttatagg tggctttaat gtaaggggta aaggtgtaga ttcatttgat gaagaagaag
    49621 attctactga gataataaga aagattgctg aaaagcttga atgtgtagtt gtagcaactg
    49681 gaaaaataga tataataact aatggtaaag gtacatataa aataaacaat ggaactgata
    49741 aattaaaagg aattacagga actggatgta tgactgctag cttgattgca agttttatgg
    49801 ctgttactga gaatatatta gaagcagcaa caatgggtgt tcttacaatg tctttaagtg
    49861 gggaattagc taatttaaat aatccaccaa taggtacatt taaagaaaat cttatgaatg
    49921 ctatttatca aatggatata gatactttaa gtaaaaattc taatattgaa tttttaaatt
    49981 aataaagagt aagggagact taaaatgatt gataaagaat ctttaaaaaa atgtttaaag
    50041 ttgtatttag taactgactc tgaaatgctt aaaggtagag acttttataa atgtcttgaa
    50101 gatgcaatat cttctggaat aacaactgta caacttagag aaaaaaatgc ttctggaaga
    50161 gagtttttaa gaaaagctat gaaacttaga gaaataacta aacaatatgg tgttaagttt
    50221 ataataaatg atagagtgga tatagcactt atttgtgatg cagatggagt acatgtagga
    50281 cagagtgaca ttgatgttag agaggttaga aagcttatag gaaacaataa aattctagga
    50341 gtatcagcaa gaactttaga agaagctatt tgtgcaaaaa atgatggcgc agactattta
    50401 ggtgtagggt ctatattcac aacatctact aaattagatg caaaatcagc ttcgtttgaa
    50461 actgttaaag aaattaaaga aaaagtagac atgccatttg tactaattgg aggtataaat
    50521 ttagacaata tagataaact taaatgttta gaaagtgacg ggtatgctat aatatcagct
    50581 atattaaaag ctgaagatat ctctaaagaa gtggaaaagt ggacattaaa aatataaaat
    50641 tggctctggt agtgaaaaaa atggaaaaat ttgatataat ctaagagtaa aatttaagca
    50701 attataaatg ataaactttt ctacatatac tttttaatca agatatatgt agatttggtt
    50761 aaaaaggagc tataaatgat taggatattg ttagtgtgtg ttggaggcat gtcttctacc
    50821 ctattggtaa ataaaatgga gaaggatgca aaaaaaagaa atatagactg taaaatatgg
    50881 gcagttggag aaggagatat aaaatcggag cttgataact ttgatatttt acttcttgga
    50941 ccacaactta gatttatgct tgatgacgta aagtctatag ttggagatag agctccagta
    51001 tctataatag atatggtaaa ctatggcact tgtaatggtc atgctgtgtt aaattcggtt
    51061 ttagaaatat taaaataaat aataaaagtg gattctatat aatagagtcc atttttatta
    51121 tttgttatta ataggcttaa aacttagctt accatttctt tttttaatct ccacatagca
    51181 agtgaaaaac ttgaacagca caatattaaa aatagtagga taacaggcag tatatctcca
    51241 ataagaatag tctcaccaaa tattaagcta cgtaaagcgt taataacatg ggtaaatgga
    51301 ttgagcatca ctgctacttt taatcctcca gataaacttt ctatagggaa aagagctgaa
    51361 cttaaaaaga aaataggaag tacaattgca gtcataattg tttcataaac tatttcattt
    51421 ggcaataata aacttataga ataagctaga cttgataaaa agaaggctgt cataaatatc
    51481 agtactatca taagtaggat tccaccaatg ccagattcta tccttactga gaagaatata
    51541 cttactatac acaaaatagt tacttctatg aaagaaacta agatagcttc taataattga
    51601 ccaaggacga ttgaatacct actgataggt gcaattaaga ctctatagaa acttccactg
    51661 ttcttcatta taaagtttat aataccacca ctactacaag aagaaaacac aaccagtaca
    51721 atgatacctg gtagtataaa agcagtataa ttattgatat ttatattatt catagtttgg
    51781 tttgcaatag aactataaag cacaagccaa agaaaaggtt gtaatatagt aactacaaat
    51841 gagattggat ttttaaaacg ccacttcata ctacgccata agatatttaa cattcccatt
    51901 cctcctttaa gcttttttta gaacttgtaa gtgctaaaaa tacatcttcg agacttggct
    51961 ctacaatttc tattgcatca aattctatat catgttctaa aagccattta tttattaaag
    52021 taaaagcagt tctactatcg tttacactta taaaaattga attttctcgt acgctcataa
    52081 actttactaa accagtactt ttaattgaat ctttatattt ttttatatct tcagtattat
    52141 gaaaagttat acgtagtata ttttgtctaa tgtaactacg taaacttgat ggtgttcctt
    52201 gtgctaaatc ctttccattc ttcataatac aaatattatc actcaattgt tcagcttctt
    52261 caagataatg agtagttaga aatatagttg tgccatactc atctctaatt tttaataaca
    52321 tatcccacat agattttctt gaatcgacat ccataccaac agttggttca tctaaaaata
    52381 ggattttagg agatgatacc atattcatag ctatgtccaa tctacgtttt acaccccctg
    52441 aatatgatga agtaggatat tttaagtaac tagataagtc aaacttatca attaaagagt
    52501 ctattctttg ttttgcaatc tgtggttcta ctttatacat tttactttga aataccatat
    52561 tttccataag ggataaatgt tcatcaatag agatttgctg tgctacacaa gcaatttgag
    52621 tacgtatcca agaaggatta tctactaaat cttttccaaa cattgttaca tttccagatg
    52681 taggtttgta aaaggtagtc aaaatattta taagagaaga tttaccagca ccattaggac
    52741 caagtaaaga aaaaatttct ccagcattta cattaaagct tagaccattt aaagctttca
    52801 caccattttt atattctttt actaaattgt ctatttcaat cgctaacata tattattcct
    52861 cctctttaag tggaaatatt atctctgtaa tatagttatc tgggtttcct tttatcaaca
    52921 tacctggtcc tttaataaat acttctctcc aaggagtttg tatttttata ttattttcat
    52981 gaatatattt aaatatcatt tcatacgttt ttggcaaatt actataagag cctgtgtgag
    53041 taaaaaataa agcttttgtt ttaggaaatt cttttgtagt tatacccata cgatttggag
    53101 tttcagcagt tggaacacat aattccattt ctgcaacacc agttttttgg tcaacatcat
    53161 agtagcaaaa gaatggtgca ccgttagact ttccttttat agctttaaat acatttggaa
    53221 aatactttgt tgcctcagtc ataggtccat tataacgcat ggtagccact gtgactgatt
    53281 cgatattttt gatttcaatt ttgcaattca tttgtttacc tcctaatgtg tgttcatatt
    53341 cttactagat ttttaagcat taatacatat aagattttga ttagttgaaa acatataatc
    53401 ttatctcgtt tgcttgtttt caagtctaac ataacataaa atatatatcc tctcatttga
    53461 tgctccattt tgtaaagtta ttatttaagc tttactttag attttttagc aaattaaaat
    53521 aaagcaattt ataaaataag tatttacaaa ttaagtgtat taatcgtata atacacttaa
    53581 taggatacta gataaagtct gaaataaagg cgtatttttt taaagtaagt gtactatcgt
    53641 attaatacac tagaatagga ggaatcatga attttgaatt agataataca actcccattt
    53701 atttgcaaat agtaaagtat ataaagagac aaatagtaac aggagagttg aaaccaggag
    53761 agacaatacc atcaagaaga gaaatggcac ttaatctaaa agtaaactta aatacagttc
    53821 aaagagcata taaggagatg ggtgatatga atataataaa tacttttaaa aattatcaaa
    53881 gcagtgtaac agttgatgaa aatatattaa aaaatttaaa attagagctt ataaatgaat
    53941 cactatctgt ttttatagaa gatatgaaag caataaatgt tagcaaagaa gaagttttaa
    54001 aaataataga agataagtat tagtttttta acaaatagaa aacttagttt agaaagttta
    54061 tttataagca tattggatgt ggaggatagt aaatatgata gaaataaaga atgtcagcaa
    54121 aacatataaa aggatgcaag ggcttaaaat aagaaagata gaagctttaa aaaatgtaag
    54181 ttttaatata gaaaagggaa aaataactgc attacttggt ataaatggtg ttggtaaatc
    54241 aactatgtta aaagcaatag caggacttat aaacattgat agtggagaaa ttcgtataga
    54301 tggagaaaaa ataaatgaaa aagtatacaa taaattggct tttgttccag atgtacaatc
    54361 tcactttagc aatacaacaa taaaagaaac atttgaattt atggaaattt tttattcaaa
    54421 gtggaataaa gaaaaatcaa aagaaatgat ggacatattt aaattggatg aagatgaaat
    54481 aattgataac ttgtcaaaag gaaacattgc aagagtaaaa ttaatacttg gattttgcca
    54541 agacccagag tacatacttc ttgatgaacc atttacagga attgatttgt ttaagaggga
    54601 agagtttata ggagtaatag cgcaatatat ggaggaaaat caagccatta tcataactac
    54661 tcatgagata gtagagatag aaagtttagt tgatgaggtt gtaattcttg atgaagggca
    54721 gattataact tcatttaatg cagaagaatt aagagaaaga gaaggtaaat ctatattaga
    54781 taagatgagg gaggtatata agaatgaata aacttctata tctaataaat gtagatttga
    54841 gaagaagcaa taagttttat atagcttata tatcattttt ttcattaatt acacttagtt
    54901 taaatttatt cgaaattaat aaatttaaaa attctaaatt tattatgaac ttagcaatag
    54961 aagattttgg aggtatattt tatggactag ggatacttag taatcttggt tttatccaaa
    55021 gtatattgtt ctttgggata ttgggacttt ttatatacta tgtttttatg tggaaaagag
    55081 agttcttttc aaataatcaa agtatttata cacttatgat gttacctcaa aataagttta
    55141 agatttgtat atctaaatca atagccttga taacaatgat atatggtttt ttgataagtc
    55201 agacagcaac attatttata tgtaaatata tatttaattt catttttaga aatatgccta
    55261 taatcaatat gaactttgcc aaagatttat attatttaaa cttgaagagt ataccaattg
    55321 attttatctc ttttatggct atttatgtat ttttactact tatagctatt tctattgtaa
    55381 attgctgtat attatttatc ttttcctata taaataaatt ttatgtaatt ttaatcactt
    55441 tgctgattat agcaggctta tatttttcaa caatatatat aaatataatc actttattta
    55501 gaaaatatta taatcttata ggatttcaat ttaacaattt agtagcaggg ttattaagtt
    55561 ttttaattat aatgtttatt ctaaatggaa tatcttattt gctaattaaa aagaaagtat
    55621 ctctttaagg aggggtggta tgaaattaaa atctaaaata tctatactta ttatattttt
    55681 gactttagca atatatttta gtataggttt attaaataat gaagataatt taaagataaa
    55741 aactataaaa ggtgactcta atgaattaga ccatataaac ataaattttg gatatgcttt
    55801 gaatggatat ataaattcat attatacaat aaatggaagc aacaaaacta aaaagactga
    55861 attaataaaa ctacaaaagg atagtaagaa cacaaataat ttaattgatg tatctagtgg
    55921 ttcagattta attgctcatg ttggatttga aatgaatgag gagtcaaaag atataaactt
    55981 tgtgatagat gagaaaaaag aagatttaga aaaaggtgta tttccatcag aagatagtga
    56041 taaagaaatt gaatatgaaa aattagatat gaataatttt acaaggacaa gatataaagt
    56101 agataaaagt acagatgatt attatgcaaa gatactatca tcaaaaagat ataaaaaata
    56161 cttatatgta tctgtacttt atgctacaag tgattcatca gaaaataaca aagaatattc
    56221 aagtactata gatataataa aaattaatcc agaaaataaa aaaatcaaga ctataaaaag
    56281 tataaattta agtaattatt tttccgaaaa ataccaaaac atagagaaca taggtgtaag
    56341 ttatgctagc aaatatgagg ataagatgta tattttagta aatgtactaa atacaaacat
    56401 aggtgataat agtaattcta aatttcattt acttgagtat aatcttgaag aaaataagga
    56461 aagcataatg gaattaaaaa ctgatagcaa aatggcttta gcaaaagcaa agttagaagc
    56521 taataaatta tatatggtaa tgtcgaatga ttatgataat agttcaatta cagagttaaa
    56581 tttacttacc tataatatat tagataaaag cttagatgaa gataaatatg ttttagataa
    56641 tagagaaaaa agaggaatta gaagtattga aattgattca aataaaatcg ttgcatatac
    56701 atcaaggcta aactctatat tatcttggga tgataaattt atgctatata taataaataa
    56761 aaattctaag gaaattgtat atgaaggaga attacaattt aacagttcaa atagtatgtt
    56821 agaatttgaa ataaagtagg tgagttgatg aaagatttaa taagattatt cgaatttgaa
    56881 ttaaaaagaa atataaagaa ttatatattt ataataattt gttgttgttc ttttgtaatt
    56941 ttaaacatag taaaaaattt aaatgactat aattacataa tagaaaatgc agttaaaagt
    57001 gaacagttaa caaaaatcgg aaatacgata gaaacggtaa atgttacagg tttctcaagc
    57061 tttagaaata taatgggttc tacagaattg tggtttatgt ttggtataat tgcatgtatt
    57121 tgctatgctt tttttatttg gtatagagac tttaatggta gaagtaaatc tatatataca
    57181 cttataatgc tacctaaaaa tagaatcaat atttatatat ctaaattatt aaatatatta
    57241 tttctagtgt atagctatac agtagtactt acaatatctt tgtttattgc aagtaagttg
    57301 ttacctagac atatgcttgg taatgtcaca aactatggtt ttgtacaaga aacaatatat
    57361 gagttaaaaa tgttacttcc atatagcttt gaaatactat ttgtagagta tatattttta
    57421 ttgataggat ttgtatctgt agtatttaca tcaattctta taaataaatc tatttataaa
    57481 gtaagcactt taataagttt tctattttta gttatagaaa ttttagtgtt tataatttct
    57541 atagaattta ttatacctta cgaatacagt gatgtattta ctgttctata ttcaagtata
    57601 aacatatttg tatgtagtct tatatctaat aaacttttga agcaaaagat tgacttttag
    57661 gagggatatt atgaaagcaa aaaatatagg aaaatataca atatgtttta taatagtatg
    57721 ttttttaatg attactatga ccttaatatt gagttatttt aataaggata aactatctgt
    57781 aaaaacttta aaaggcaatg taaaagatat agggagagta agtgtagtat atacacctaa
    57841 tataagcaaa ttttcttatg aggaggtaat tataagtaaa aatggagtaa gaaataatac
    57901 aaacccaaaa tcaagaatta gaaatgatta tttaaatatt gacaaaaaat acagtgactt
    57961 tgttaatgat aaaggaaaag aatttatata taaagatgat ttgaatatag gagaagcgta
    58021 tacctctgtt aagtctgatt tagtacaagg aatgaatgct ttaaatgatt gtataaaggt
    58081 ttcaaataaa aatttaaaaa gtggggtaga agaaaatttt gatattaatt taaataagtc
    58141 tgattttaaa gaaaatagtt ggaaaacctt atttaacaaa atatataaag gtaatttata
    58201 tatggtaatt agtgaaacta atatttcagg ttctaaagtc aataatattt gtatattaaa
    58261 gatagatata aaaaataagt cttataaatt attaaataga tttaatttag gattacagaa
    58321 tggagaacaa caaaatatca atgaaattaa attttctatt ggaagtaaat tatacttaga
    58381 aatagtaaat aataaaagta aaaatggata tattgaagga catgatagtt actctaactt
    58441 tattatatat gatatagaag ataacacttt taatataagt aataaattta tggataatct
    58501 aaacaaagaa aagaatgcaa atccaaagga gattggctta ttaagtaaaa ctaagctaga
    58561 ttatatggta aaaaacaata aattgaatct tatagttacg gatatgagta attccataac
    58621 taatcttgtt tatgatatta attctaatga gattaaatta gaagattatg agatacttga
    58681 tttaaaagta gattataatg aagcatcatc tggagttagg aaagttaagc tactggatga
    58741 taagatatat tctatttcag gtattaagaa cagtatatca ggagaattaa aaggatttaa
    58801 agggattgtt gatgtagtcg gaactaagcc agtaaaattt caagtatttg atatgaaact
    58861 taaaaagaat atttatgaag ctgagctaat aaatggagat ataagtttac aagaaaagtt
    58921 attttttgtt gcaaattaag tgttagaatt aattttagaa ctttaaactt ttatatgtat
    58981 cacagataag tataagtaag ggagtgtctc aaaataaact ttttagctca tgagacactt
    59041 tttttatatg aaatcaaata tattttcatt gtttagattt attttgataa tgaaaaaa
//
LOCUS       DAMJWS010000013        57470 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMJWS010000013 DAMJWS010000000
VERSION     DAMJWS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.013"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            194..1270
                     /locus_tag="PC399_000882"
     CDS             194..1270
                     /locus_tag="PC399_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2591758.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1387..2229)
                     /locus_tag="PC399_000883"
     CDS             complement(1387..2229)
                     /locus_tag="PC399_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2591759.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            2413..2985
                     /locus_tag="PC399_000884"
     CDS             2413..2985
                     /locus_tag="PC399_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2591760.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3148..3420)
                     /locus_tag="PC399_000885"
     CDS             complement(3148..3420)
                     /locus_tag="PC399_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591761.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3547..4419)
                     /locus_tag="PC399_000886"
     CDS             complement(3547..4419)
                     /locus_tag="PC399_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2591762.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4706..7813)
                     /locus_tag="PC399_000887"
     CDS             complement(4706..7813)
                     /locus_tag="PC399_000887"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF2591763.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7906..8138)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8313..8840)
                     /locus_tag="PC399_000888"
     CDS             complement(8313..8840)
                     /locus_tag="PC399_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF2591764.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8875..9660)
                     /locus_tag="PC399_000889"
     CDS             complement(8875..9660)
                     /locus_tag="PC399_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF2591765.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9670..9927)
                     /locus_tag="PC399_000890"
     CDS             complement(9670..9927)
                     /locus_tag="PC399_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF2591766.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9946..10404)
                     /locus_tag="PC399_000891"
     CDS             complement(9946..10404)
                     /locus_tag="PC399_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF2591767.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10444..11148)
                     /locus_tag="PC399_000892"
     CDS             complement(10444..11148)
                     /locus_tag="PC399_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF2591768.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11158..11793)
                     /locus_tag="PC399_000893"
     CDS             complement(11158..11793)
                     /locus_tag="PC399_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591769.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12174..13214
                     /locus_tag="PC399_000894"
     CDS             12174..13214
                     /locus_tag="PC399_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2591770.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13677..15032)
                     /locus_tag="PC399_000895"
     CDS             complement(13677..15032)
                     /locus_tag="PC399_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF2591771.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15058..15327)
                     /locus_tag="PC399_000896"
     CDS             complement(15058..15327)
                     /locus_tag="PC399_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2591772.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15617..17572)
                     /locus_tag="PC399_000897"
     CDS             complement(15617..17572)
                     /locus_tag="PC399_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591773.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17755..19230)
                     /gene="spoIVA"
                     /locus_tag="PC399_000898"
     CDS             complement(17755..19230)
                     /gene="spoIVA"
                     /locus_tag="PC399_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF2591774.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19402..20697)
                     /locus_tag="PC399_000899"
     CDS             complement(19402..20697)
                     /locus_tag="PC399_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2591775.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(20881..22290)
                     /locus_tag="PC399_000900"
     CDS             complement(20881..22290)
                     /locus_tag="PC399_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2591776.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(22342..23481)
                     /locus_tag="PC399_000901"
     CDS             complement(22342..23481)
                     /locus_tag="PC399_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2591777.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVEGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            23800..24450
                     /locus_tag="PC399_000902"
     CDS             23800..24450
                     /locus_tag="PC399_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2591778.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(24607..25626)
                     /locus_tag="PC399_000903"
     CDS             complement(24607..25626)
                     /locus_tag="PC399_000903"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2591779.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(25657..26271)
                     /gene="plsY"
                     /locus_tag="PC399_000904"
     CDS             complement(25657..26271)
                     /gene="plsY"
                     /locus_tag="PC399_000904"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF2591780.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(26306..27625)
                     /gene="der"
                     /locus_tag="PC399_000905"
     CDS             complement(26306..27625)
                     /gene="der"
                     /locus_tag="PC399_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF2591781.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(27659..28987)
                     /locus_tag="PC399_000906"
     CDS             complement(27659..28987)
                     /locus_tag="PC399_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF2591782.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADTHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(29225..29425)
                     /locus_tag="PC399_000907"
     CDS             complement(29225..29425)
                     /locus_tag="PC399_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591783.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(29427..30359)
                     /locus_tag="PC399_000908"
     CDS             complement(29427..30359)
                     /locus_tag="PC399_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2591784.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(30404..31336)
                     /locus_tag="PC399_000909"
     CDS             complement(30404..31336)
                     /locus_tag="PC399_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2591785.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(31391..32680)
                     /locus_tag="PC399_000910"
     CDS             complement(31391..32680)
                     /locus_tag="PC399_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2591786.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(32709..33401)
                     /locus_tag="PC399_000911"
     CDS             complement(32709..33401)
                     /locus_tag="PC399_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591787.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(33416..34177)
                     /gene="pgeF"
                     /locus_tag="PC399_000912"
     CDS             complement(33416..34177)
                     /gene="pgeF"
                     /locus_tag="PC399_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF2591788.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(34174..34635)
                     /gene="nrdR"
                     /locus_tag="PC399_000913"
     CDS             complement(34174..34635)
                     /gene="nrdR"
                     /locus_tag="PC399_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF2591789.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(34657..34920)
                     /locus_tag="PC399_000914"
     CDS             complement(34657..34920)
                     /locus_tag="PC399_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2591790.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(35024..35797)
                     /gene="sigG"
                     /locus_tag="PC399_000915"
     CDS             complement(35024..35797)
                     /gene="sigG"
                     /locus_tag="PC399_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF2591791.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(35889..36611)
                     /gene="sigE"
                     /locus_tag="PC399_000916"
     CDS             complement(35889..36611)
                     /gene="sigE"
                     /locus_tag="PC399_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF2591792.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(36636..37508)
                     /locus_tag="PC399_000917"
     CDS             complement(36636..37508)
                     /locus_tag="PC399_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF2591793.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(37798..39072)
                     /locus_tag="PC399_000918"
     CDS             complement(37798..39072)
                     /locus_tag="PC399_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2591794.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(39356..40513)
                     /gene="ftsZ"
                     /locus_tag="PC399_000919"
     CDS             complement(39356..40513)
                     /gene="ftsZ"
                     /locus_tag="PC399_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF2591795.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(40703..41056)
                     /locus_tag="PC399_000920"
     CDS             complement(40703..41056)
                     /locus_tag="PC399_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF2591796.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(41155..41829)
                     /locus_tag="PC399_000921"
     CDS             complement(41155..41829)
                     /locus_tag="PC399_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2591797.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(41834..42532)
                     /locus_tag="PC399_000922"
     CDS             complement(41834..42532)
                     /locus_tag="PC399_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2591798.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIEIYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(42555..43295)
                     /locus_tag="PC399_000923"
     CDS             complement(42555..43295)
                     /locus_tag="PC399_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF2591799.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(43565..44791)
                     /gene="murG"
                     /locus_tag="PC399_000924"
     CDS             complement(43565..44791)
                     /gene="murG"
                     /locus_tag="PC399_000924"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2591800.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(44809..45939)
                     /gene="spoVE"
                     /locus_tag="PC399_000925"
     CDS             complement(44809..45939)
                     /gene="spoVE"
                     /locus_tag="PC399_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF2591801.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(45991..47346)
                     /locus_tag="PC399_000926"
     CDS             complement(45991..47346)
                     /locus_tag="PC399_000926"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF2591802.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASVIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(47365..48333)
                     /locus_tag="PC399_000927"
     CDS             complement(47365..48333)
                     /locus_tag="PC399_000927"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF2591803.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(48374..49747)
                     /locus_tag="PC399_000928"
     CDS             complement(48374..49747)
                     /locus_tag="PC399_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF2591804.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(49914..51893)
                     /locus_tag="PC399_000929"
     CDS             complement(49914..51893)
                     /locus_tag="PC399_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF2591805.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(51905..52252)
                     /locus_tag="PC399_000930"
     CDS             complement(51905..52252)
                     /locus_tag="PC399_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591806.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(52738..53673)
                     /gene="rsmH"
                     /locus_tag="PC399_000931"
     CDS             complement(52738..53673)
                     /gene="rsmH"
                     /locus_tag="PC399_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF2591807.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(53703..54449)
                     /locus_tag="PC399_000932"
     CDS             complement(53703..54449)
                     /locus_tag="PC399_000932"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF2591808.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(54843..56129)
                     /locus_tag="PC399_000933"
     CDS             complement(54843..56129)
                     /locus_tag="PC399_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2591809.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LEYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(56144..57409)
                     /locus_tag="PC399_000934"
     CDS             complement(56144..57409)
                     /locus_tag="PC399_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2591810.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSSKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
BASE COUNT        17900 a        10515 c         6094 g        22961 t
ORIGIN      
        1 atttattact tttatagaat tttcacaata aaaagtcatt ttacttccgt atttaatgca
       61 aaagtagaga ttaaacttaa tataaaaaat atataacaaa ttttttttat atttctagaa
      121 gaaaagtata ctcttttttg tttaataatg ctaaaattga tttgtattga attttgatta
      181 ggaggaaatt attatgaaag ctcaaagatt aaatgctgta cttgaacaaa tgaaaaaaga
      241 tgatatttct caaatgttag taagtgaccc tacttctata ttttatctta caggagtact
      301 aatccatcca ggcgaaagac tattagcatt atacttaaat ttaaatggca ataataaatt
      361 atttataaat gaattattcc cagtttcaga agatttaggt gttgaaatgg tttggttcaa
      421 tgatactcaa aatccagtag aaataataac tgaacatata gacaaaaatg ctactatggg
      481 tgttgataaa aactggccag ctagattctt attaaactta atagaattag gtggaggaag
      541 taagtttgta aattcatctt atataataga tactttaaga atgtgtaaag atgaagaaga
      601 aaaagagtta atgagaatag cttcaaaatt aaatgacaag gctatggaac aacttaaagc
      661 tactgtttct ggtgaactta cagaaaaaca acttgttggt aaattatcta aaatatatga
      721 agacctaggt actgatggat tttcatttga ccctattata ggttttggac ctaatggagc
      781 taatccacat ggtgaacctg gaaatgcact tgtgaaacca ggagatgcaa taatcttaga
      841 tataggttgt ataaaagata actattgtgc tgatatgaca agaactgtat tctataaaga
      901 aatacctgaa aaaggtagag aaatatttga aatagttctt gaagctaata agagagctga
      961 agctattgta aaaccaggtg ttagattctg tgatatagat gctgctgctc gtgactacat
     1021 aactgaaaag ggatatggac agtacttcac tcatagaact ggtcactcta taggacttga
     1081 agttcatgat aaaggagatg tatcttctat aaatacagat actgtacaac caggaatgat
     1141 attctctata gagccaggta tatatttacc aggtgagttc ggagttagaa tagaagattt
     1201 agtacttgtt actgaagatg gttgtgagat attaaacaaa catgacaaag aaatatgtgt
     1261 agttggttaa ttctaaaatc aatttgataa aattaaaaag ccatataaaa atgttttaaa
     1321 catttttata tggctttaat ttctcaatct atgatttatt tcaatccatt atttatattg
     1381 cagtatttat attgcagtta ttaagattgc accctttgaa gtatatgtcc ccatcactga
     1441 accaacttca cagattacaa catctttgta ttttacatcc ttttccataa cttcccttac
     1501 cttttgagcc ttttctgggc aattagcatg tcctataaat aaaacctttt cttctttatc
     1561 tttaattttt gactctacta aagtaatcag ctttttcaaa ctgtttcctt cacctcttgc
     1621 cttatctata ggagttacaa cgccatctgc aacttgtatt atcgccttaa aatttaaggt
     1681 atttataatt tttcctgcta ttggatttat tctaccaccc ttaatagcat tttctaaagt
     1741 ttcaagtgtt ccaaaaaatg gaggattatc tttatattta tttattattg aaacaatctc
     1801 ttcaatttct tttccttctt ttattaatat tgctgctttt aacactaatt gtcctattcc
     1861 aattgaccca ctaagtgaat ctataacctc aactttcttg ttatttcctt ccttttcaaa
     1921 catatctttt gctaaaagtg cactattata agtcccagat aatcctgaac ttatattaat
     1981 cactaataca ctttcttcct gacaatggta tgcttctaaa aatttatcag gagaaggaca
     2041 tgatgttttt ggtagtactt tactctcact catttttcta tagaaagttt cattatctat
     2101 ttcctttcca gatatataac tttcttctcc aaaagataca tttaaaccaa caacttctat
     2161 gttgtatttt tctattatat catcaggtaa gtcacatgca ctgtccacta tcaattttat
     2221 tttatccata ttaacctcac tataagatat tattttattt attcagtcgg tcgtacgacc
     2281 gatttaatta ttattttata tattgaatga aaaaaagtca atagatatag attctcgctt
     2341 tattgacaat aaatatccat aagcttataa tgttattaat ttgttataaa tatacatgga
     2401 aaggtaggtt ttatgaaaac aaataatatg aaacaggcta ttttatcatc tgcaaccaat
     2461 ttaatagcca aaaatggagt tcaaaatact agccttgctg atattgcaaa agatgtaaat
     2521 ataagtaaag gtactttgta ttatcactac gcatctaaag atgatattat atatgatatt
     2581 gcggatactc atttagaagt tataactagt gctatactaa actgtgtaaa aaatgtcaaa
     2641 tcaaaaaatt ctcaaataga aatggttaac ttaatcttag agaaaatatc tactatagaa
     2701 agtcgtggta gggtacatat gtatcttatt tgtgaggcaa ttacttcaaa taatgattta
     2761 aaggaaagaa taagattaaa atatattgaa tggagaacta ctcttaaatc tgaaattgtt
     2821 gaatcactag aaaaatataa tagtagcgaa aatgaacagg atgctgaatc attttctttt
     2881 ttactcatgt caatagttga tggtttagtt gtccaaagtt tattaaaaac agaaaaaata
     2941 ccttatgaaa atatagcttc ttttttagtt aatcattgga tttaaactta atacttagat
     3001 aattaataaa agtgaactat ttaagttatc tactttaaca gttcactttc ttatttattc
     3061 tcaataattt ttacttaaaa cattcaattt ttatttaaac cttcatggaa tcacaaaata
     3121 aaaatctctt ataaatttaa aatgttttta tttatgttca tgtgtatgcc catctgaatg
     3181 ttcatgacca tgtgtatgtg tatgctcatg gctatgctca tgagtacctg aaaaaccatc
     3241 atcatgatta tggtcgtgac ctacaccatt tacatgctca tgttcatgcc catgactgtg
     3301 agaatgttga tgtgaatgtt catggttatc tcctccacag catccttctc ctccagtccc
     3361 acaacaccca cttgtatgtg gattatgatg atttcttact acaaatctct ctcttaacat
     3421 aattcttata cctcctagta tattattgag tacttcatgt accccattaa ctatggtata
     3481 catctaacta ggtataatct caatacatat gtcatttttt aacatatata attaacatat
     3541 ataatcttaa agaaatttat ccttaaaacc tccacctttt acttcttcaa cgttattgac
     3601 agaaataaaa gcttcactat ccatatcata aattaaatct ttaaatttac ctatttcatt
     3661 tgcagatatt atgcaataaa tcatcttttt cttattttgt gtatatgcac cttctgcctc
     3721 caaaaaagtg actcctctac ccattttatt cattatcact ttggatattt catcatattt
     3781 atttgatatt aaaagtatag atttttttct atcaaagcaa tcttttccta tatccatagt
     3841 aaaagaaatt atatacattg taattaaagt gtacatcacc aatttagctc caaataaaaa
     3901 agctcctgca catactacaa aaaaattaat aaataaaaat gtatttttca taggtatatc
     3961 atactttctt ttcattatgg ctgctattat atcaagtcct ccagcagtag ctcttgcttt
     4021 aaaagttaac cccataccta taccagttaa tgcaccacca taaatacact gtagcatagt
     4081 atcatctatt gggaaatatt tagcaatatc ttgtgttagc cccagtgcta gagaaaatag
     4141 taacatattg acaaaactta ttagcaaaaa tttattatca aaatactttc tagctataat
     4201 aaatattggt atgttcatca agaaacttga tattccctga tttataccaa ataatttgta
     4261 taatataaca caaattccag ctactcctcc acttaataac ttggctggtg ctagatataa
     4321 atttattcct aaggttgata gaatcatacc caatataatc agacaatatt gtaaaaatat
     4381 attattatca atacttagct ttttactttc accatacatc aaaacgcacc cctttgcata
     4441 ataaataaac caccctgcat aacacacgat aaatttacaa ataaatttat cagaagtagt
     4501 ttcctaaaaa tattattcat cctcacttaa acgtttattc attaatattc tatcacatga
     4561 cataaataat ttatatgatt tatacagatt tatttattgt aaattttgga atattaaaac
     4621 attttattga atagataatt atattacata aaaaatccct atagaaatct ataaactaat
     4681 ttctataggg attttgatta actaactatt tttttataac ttttatacct gtcatatggt
     4741 catttaagtt ttgttcctct aaagatttat catctactct gtttatttct aaagctaaag
     4801 tttcagactt gatatattca ttaaaatggt caacagcttt agcaatttca tcatcaccac
     4861 agtattctat tactatatta tctaatactt caaagttatt agattttctt aattgctgta
     4921 ctttagatat aaattctcta gcataacctt cattaatcaa ctcttcagtt agcttagtat
     4981 ctagtataac aaaaagattg ttttcaacag atacattaaa tccttcctta gcagttatac
     5041 ctattaatac taaatcctta ttaaattcaa aagcttcacc atctaaatca actgttaaag
     5101 tctcaccttt ttcaagttta ttagcagttt catgagcatt taatttactt aatgctccaa
     5161 cgaataaatt catcttagaa cctaataatg gccctgcttc cttaaagttt ggttttaaac
     5221 taaaattcat atactcatct aaatctttag caaatattac ctctttaaca ttaagctctt
     5281 cttttattaa atcaactaca tcacttattg tatcttcaaa cttaccatct actaacactt
     5341 tttgtaatgg ttgacgaact tttattcttt ctacttctct tgaagctcta cctaaagtaa
     5401 ctaagttttt agctaaatcc attttttctt ctaatttagt atctactaac tcttcattac
     5461 atttaggata ttcagctaaa tgaacagata cttctcctgt taagtttcta tacatttctt
     5521 ctgacatata tggtgcaaat ggagctatag ctctacaaag accatgtaac aattcataag
     5581 ttgtattgta cactgctttt ttatcttctg ttaattcagt tgcccaaaat cttcttcttg
     5641 aacgtcttat ataccagtta gataaatcat catttataaa atcttgtatc attcttaaag
     5701 ttttatttaa ctcaaatata gctaaatttt cttctacatc tttcattaaa ttattgaact
     5761 tagataatat ccatctatca agttctggtc tatctttata ttctacaaaa aattcagttg
     5821 gatttatatc atctgtgttt gcatataaag taaagaagtt atatacattc ttcatagtac
     5881 ctataaattt actttggatt tcttttaatc catccatatc aaatttagtt ggagtccatg
     5941 gtggagaaac atatagtaaa taccatctta atgcatctgc tccatattgg tcaaataatt
     6001 ccattggatt tactgtattt cctcttgatt tactcatttt cttaccatct ttatcaagta
     6061 caaggtctgg aactaaaaca ctcttatatg gtgcttttcc taatacaaat gttgatactg
     6121 caagtaatga gtagaaccaa cctctagtct ggtctatacc ttctgatata tagtcagctg
     6181 ggaacaattg gtcaaagttt tccttatttt caaatggata gtggtgttga gcaaatggca
     6241 tagccccact atcaaaccaa cagtctataa cctctgttac tcttgtcatt ggttttccac
     6301 atttttcaca ttttaagtgt atatcatcta catatggtct atgaagttca actgtttctg
     6361 gatttacatc ttctatagct ttttcagcta attcagctct tgaaccaact gaatctgtat
     6421 gaccacattc acatctccat ataggaagtg gagttcccca atatctagtt cttgatattg
     6481 cccagtcatt tagattttct aaccagtttc caaatctctt ctctcctaca aaacttggga
     6541 accattttac accattatta ttttctatta atttatcttt aagtttagtc atctctatat
     6601 accagcttgg ttttgcatag tatactaatg gagtttgaca tctccagcag tgtggataat
     6661 tatgaactac tttttctttg ctatataatt tattttcaga agctagccat tttattattt
     6721 caacatctag cccatcttcc attacaaatc taccttccca aggagtagat gtaaatttac
     6781 cagtttcatc tactggttga ataactggta aatcatatct tttacctgtg ttgtagtcat
     6841 cttcaccaaa tgctggtgct gtatgaacta taccagttcc atcttcagta gttacataat
     6901 ctcctactgt aataaaaaat gcttttttgt caacttctac aaaaggcatt aattgttcat
     6961 attctaaacc ttctaggtct ttacctttca tttcttctaa aacttcataa tcttcaccta
     7021 gtactttatc agctaaaggt ttagatacat aatatacctc atcacctttt ttaactttta
     7081 aataatctac atcagcattt actgttaatg aaacatttga tggtaaagtc catggagttg
     7141 ttgtccatgc taagaagtat tcatcagcat cttttttctt aaatttagct ataactgtat
     7201 tagtttttac ttctttatat ccttgtgcaa cctcatgact agcaagacca gttccacatc
     7261 ttgggcaata aggaagtatt ttatgccctt catatatata tccttctttg ttgaatttgt
     7321 ttagtatcca ccatacagac tctatatagt tattatctaa agttatatat ggattatcta
     7381 aatcaacttc ataagccatt ctctcagtca ttattctcca ttgtttttca aatgagaaaa
     7441 ctgactctct acatttttgg ttaaattcag ctattccata ttcttctatt tgttgcttac
     7501 tagtaagtcc taactctttc tctacttgta tttcaactgg taatccatgt gtatcccaac
     7561 cagcttttct ctttacttga taaccagaca ttgttttata tctacaaact gaatctttaa
     7621 gcgttctaga taaaacatgg tgtactccag gatttccatt agctgttgga ggtccttcat
     7681 agaatacgaa acttgggtca tctttccctt tttctaaagt tttttcaagt atatttatgt
     7741 ctttccaata atcaaaaact tgtgcttctg cttgttttac agaactgtct accaatggct
     7801 taaatttcgc catgtaaatc accctttcct actaaatatt ataattccaa aattttttat
     7861 tctttcacat aaatttaaat actaaaaaac tcgccccctt taaaccaaag ggacgagtta
     7921 taaatcgcgt taccacccta attctatatg taaactaaat actagctaca tataacactc
     7981 taatagtata acggtactaa ccggcacagc ttagtagaat ttaatcgttc agcctgcagc
     8041 tcaagagtga tttttatctt tataaattca ttggttctca gcatacccaa ttctctgtaa
     8101 aattatctaa agataaatgt ctctatcaac gcttttaaaa atttatctgt gtttatatga
     8161 aattctataa caaataattt gaatagtcaa tatatttttg aaaaatataa taatttctat
     8221 ttttcttaca tttttatcct atttttaatc tttgttatag atttctcaaa ctcacacaat
     8281 tattctacat aaaaaataaa caaattacaa gcttattcta aagttgtagc agcttcatca
     8341 ttactataat tatacactgt tgttccttca aatgcgttta cacatttttc atctacatca
     8401 taaaaaatct catcaacact tcttatttca tcttctaaca gtgatttaaa tttatttcta
     8461 aatatcttaa attctttgac taaagcatca tactcttttc ttatctctat aacacggtta
     8521 ttagcttgtt ctataatttg tctagctttt aaatcagctt cttcaacaat tatcttagcc
     8581 tttttattag ccgcgctaca agtgtcttct gctgcacttt gagcagttat taaagtagct
     8641 tttagtgttt cttcaatatt ttcatacttg tttacttggt cttgatataa ccctaatttt
     8701 tctttaagag ctgtattttc cctacataga gattcataat cttccttgat gatatctaaa
     8761 aattcatcaa cctcttcatc tctataccct cttaaacctt ttttaaactc tttattttct
     8821 atctcaattg gagttagcat tacatttcct cccagctata atttaaatta gtccttatac
     8881 aattaatctt gcttgaactt ttatttttcc ttttttagta atatctccta tttgtgttac
     8941 cttagctctt cctttgcctc taatagaaat caaatcatca ttttttacta ttttagaagt
     9001 tgaagttatc ttttcataat tgacatggac tttttctcca tttatgtatt ttgcactatc
     9061 ttgtctagat atattgtaaa taccacttat cacacaatct aacctatctg aagatactgt
     9121 aaatgatact tctttatatt ttgaagaact attaacaata ttttctctgg aaatttcctt
     9181 tacaattaca ttatttcttg aaaccttttc taagtttaca attataaaat cgcaaatatc
     9241 actacttaca actacttgac aaaagctatc atgtataatt atatctccaa ttttttctct
     9301 ttttatacct agacccagta ttgaccctaa gtaatcttta tgagatatat ttttaaattt
     9361 gaaattccct tctatttgta aaaatctaag agtatcttct atatcttcat attccatgta
     9421 aaatggataa ataaatacag tgcttctttc agcttgttca taacctccat ccacactgta
     9481 ttttatttca tttgtagaat ttaatattgc tacagcattt ttcacttcat atggattaag
     9541 aaactccgta gacttaacat catagttttt tatacaacta ttagccttgt ctatcacttt
     9601 aaacatttta tttttcaagt ctatatcctt tatgtgattt gttagtttta atttatccat
     9661 cctatcacct tagaatataa ccaatactaa ttgtcttagg aacatcaata atactattgc
     9721 aattaaagga gaaaaatcta taggtccatt tgtatattta tacattatag accttattgg
     9781 cccttctatt ggctctgtaa gattttccaa aatatcaaaa attttacttt gtcttccatt
     9841 tggaaaccaa gtcatcaagc tttttataac tatcatccat gctattatat ctaaaagata
     9901 ataaagtgca attcttattg ttcccatata ttaaccgcct ttttactatt tattttgcca
     9961 agggaagaat gctttacttt ctaattcttt tttcatattt gcatctatat ccacattatt
    10021 aggagctagt ataaatattg atttagaaac cttttgtata cttccatcta agacataaac
    10081 tgccccattc ataaagttaa atatagattt acgaacttca gaattagtca atccttccaa
    10141 attcactata actgcccttc tttgttttag atggtctgct atagttgcag cttcatcata
    10201 aaccttaggt tcaactatta caactttcat ttggctagtt gtatgtaaat ttacaatttt
    10261 atttgctttt gcagtactaa agccactgtt catttcttct tcttgaacta tatcgtccat
    10321 gccactttca tattcatctt caatgtaatc ttcttcttca tcaactatcc aattcttaaa
    10381 tttagatatt attccatttg acattattaa cttcctcctt aaaatttatt caaatttgag
    10441 taattatttt ttattgtagt ttctttctcc aaagattgat gttccaactc taacaatggt
    10501 agccccttcc tctatagcaa tttcataatc gtgactcatt cccattgaca aaacatccat
    10561 ccctacatta gggatgttta gttcttttat ttgttttgat aaatctttaa gacctttaaa
    10621 tacacctctt atctcatctt catcttctat aaaaggtgcc attgtcatta gaccctttat
    10681 ttttatattt ttatattttt ctgacacttc tttgacaaag tctacaactt cttctctatc
    10741 aagtccatgc ttagactctt ctttagatat attcacttgt actaagcaat ctatttctct
    10801 gtttatttgt tctgcacgtt tttgaatttc ttcgcataat gcaattctat ctaaagagtg
    10861 tatcatacaa actttatcga ttatgtactt aactttattg gtttgcaaag ttcctatcag
    10921 gtgccagttt aatttatcac ctataatatc atactttcta gctaattctt ggggtttatt
    10981 ttcaccaaca gaagtgattc cttccctcat tgcctcatta actaagtcta catcaacagt
    11041 ctttgtgact gccaaaaggg taatctcttt gctgtctcta tctacttttt ttgcagcttt
    11101 atcaattttg tttcttatct cagacaaatt gtctttaata gaacccatta ttatcaccta
    11161 ctttatcctt atatttgtgt ttatactgtt aggtcttaaa attattttgt catatagatt
    11221 aacacttgta actccattta tgtcattttt ataataatcc acaaatacag aagattcatt
    11281 ctcataagtt attccttcta atatgacaaa ttttacactt tgtgattgtt catcaattac
    11341 atatacacca cgtttattgt cttttgtttt tatagaagtt ttaggtattt ttaaacattc
    11401 catctgatta tatattatat caaattcttc gacacttgta tcctgatttg caacaatttg
    11461 gttacttatt tttaatttta ccattatttt attattattt tcatatattt tgtaaacttt
    11521 tgcatctaat ttttgctcat gatattcaat tattacattt tgattttttt caaagttatt
    11581 agaatcttct ttactgatgt atgtagatag ataattactc tggtcattgg catctttttc
    11641 tatagttcct tttacatgaa tctttgcatc aactttttca ttttttaaca ccaacgtatc
    11701 tacatttttg ctaattatca ttactaatat attaaatact aaatatataa gaataccaag
    11761 tatcactaat ctggtatatt ttaccttttt caaagactct ccccctataa aattactttc
    11821 attataagta cttcttctaa aaaatgttta ttcctttttt ataagctaat attttacgtt
    11881 ctaataatta ttttttccat attaaattta ttttttatca atcttcctat acaagactgc
    11941 attatttata tataattgta ttataaagta taagtttaat ttacatttta tacgttaagc
    12001 ttactttata tgtttttata gttattttta tttttagatg caatataaaa ccaattaaga
    12061 tttaaaataa cttttgttat gttgacattt atttaggtaa tcatttatga attaggataa
    12121 cgttatcata acaaaagttt atgtcctaaa aaaaatacaa ggaggatttt cttatgtcag
    12181 aaattttatt aggaacaggt ttacttttat tggttctatg ttgtttcaca atttttagtt
    12241 acaaggcccc atatggtatg aaggctatgg gagctctagc aaacgctgca tgtgctactt
    12301 tcttagtaga ggcattcaac ctttctttac ttggtgatgt tttaggtatg gaatttttta
    12361 gaagtgttgg agctgctaat ggtagtcttg gtggagttgc tgctgctata cttgtgcctt
    12421 tagccttagg tgtatctcca gtttatgcag ttttaatagg attatcttgt ttagattttt
    12481 ctatattatc tggattcgtt gctggatact ttgtttcttt cttaattaag tttatagaga
    12541 aaaaagtgcc acctggagtg gatttagtgg ttgtaattct tctatgtgca ccgctatctc
    12601 gtcttatagc atcaaatgta gacccattag ttaatagtac actactacaa attggacaag
    12661 ttttgataga agctaccaac attagcccta taataatggg aattatacta ggaggtctaa
    12721 taacagtagt tggaacgtct ccacttagtt caatggctct tacatctata attggtctta
    12781 ctggtcttcc tatggcaata ggagcattag gtgcttttgg ttcagctttt atgaactcaa
    12841 cactatttgc aaaattgaaa ttaggttcta aaaaagatat tatatctgtt gctattgaac
    12901 cattaaccca atcagacata atttcagcta atccaatacc tatatattct actaatttct
    12961 ttggaggagc cttttctggt atcattgtag ccttgatggg tcttgtaaac atgacaccag
    13021 gtacagctac acctgtggct ggttttgcta taatgtttgc ttataatcca gttcaacaag
    13081 ttcttataac agctggttta tgtgctttat tcaatgtaat tgcaggattt ttaggttcaa
    13141 aaatattcag tacctttaaa gttcttactg ctggtgaaat acgtggtact gatgtagaag
    13201 aagaaattgc ttaaaaaaca ataagattta taaatttata tctcaagtag tagagtttta
    13261 tattgtaggc aattttatta attgcaaacg ttttattttt tatttatgtt tatgattatt
    13321 atgtgtgttg aatatttaga taaattttta tttattattt ttatttagat ttttgtttag
    13381 ttttttattt ggttgatata ttaggaataa aaattagtat agtttattac tagttagata
    13441 gcataacttt ttagatacat aaaaaatttt atattaatta gatattatta aaataaaagc
    13501 gatacatgaa tttttaaatg tccatgtatc gctttatttt tgccataatc ttaacaactg
    13561 atttttaaag tttattatct taacaaagtt cattatctta tataatattt aaatgcatag
    13621 acttctttaa tttaaccatc taaatattta attaaatatt attatatgta atataactaa
    13681 tttttaaatg aatgtatagg agctggaatc cttcctcctc tttgagtaaa taattcagat
    13741 gaatttgtat ttataggcat tactggtgct cgtcctaaaa gtcctccaaa ttctaccata
    13801 tcacctacat cacatcctgg ggctggtata attctaacgg ctgtagtctt attattaatc
    13861 atacctattg ctgcctcatc agctatcatg gctccaagcg tacctgctgt agtatttcct
    13921 ggcacagcta tcatatcaag acctacagaa caaatagcag tcattgcttc aagcttatct
    13981 atacttaaag cacctttaat aacagcatca atcatacctg catcttcaga tactggtata
    14041 aacgctccac taagtcctcc aacatgtgaa catgccatta ctccaccttt tttaacagca
    14101 tcatttaaaa gtgctaatgc tgctgtagtc ccatgagtac caacaacttc aagacccatt
    14161 tcctctaata tatttgctac cgaatcacca atagcaggtg taggagctag tgataagtct
    14221 attatcccaa atggaacatc caatctacta gaagcttcct ttgccacaag ttgtccaact
    14281 cttgtaattt taaatgcagt ctttttaact gtttctgaaa caacatcaaa aggctctccc
    14341 tttactttct ctaaagctct cttaacaaca ccagggccac ttacaccaac atttataatt
    14401 ctatcagcct caccaacacc atggaaagct cctgccataa atggattatc ttctactgca
    14461 ttacaaaata ctactaattt tgcacaacca aaaccttttg catcttttgt taattctgca
    14521 gtttctttta tgatttctcc catttctttc actgcatcca tatttatacc acatcttgta
    14581 gaacctacat ttactgaagc acatacttta ttagtacttg ctagagcttt tggaattgac
    14641 ttaattagta tcttatcacc ttttgtatat cccttttgaa ctaatgctga gaatccacca
    14701 ataaaatcta tacctaaatc ttctgcagcc ttatccaatg tctgtgcaaa ctttacatag
    14761 tcattttcat ctgttgcacc tgcaataatg gaaattggag ttactgatac ccttttattt
    14821 acaattggga taccaaattc acgctctata tcttttccaa ccttcaccaa atctttagca
    14881 gaactcataa tcttattgta tattttttct ctagctttat ctccatcagg gtctatacaa
    14941 tctaataaag aaatccccat agttatagtt cttatatcca actgttcttc atctatcatc
    15001 tttattgttt caagtatatt tcctacattc actattatca cctcccctta ctgtctacta
    15061 cagattatgc attgaattaa atatttcttc tcgctgaatt tttacatcca ctgataattt
    15121 cttacctctt tcaaccaagt cctcaaccgt tttctcaaaa gaatttaatg atttctctaa
    15181 atcaactatc ataatcatag taaaataatc ctgtaatatt ttttgtgata tatctactat
    15241 gttgatattt tgcttgtata attcatcact aattcctgcc actatacctg gtttatcctt
    15301 tccaattact gttaaaattg ctttcataat aatatcctcc cttatttttt tataaatctt
    15361 atatttttac aaattgaact atgtgttaac atatatttca tttatttgtt agtaaaccac
    15421 tttttatgta tatatttaat atattttaat acctatacat tcatttgtca attttttttc
    15481 aatattctat tatttttata ctatttagaa aattcatata ttatatatgt tttctcaaca
    15541 acaaatacag ttttcataat aatgaaaggt attacttagc aatctcatga ttttctaaat
    15601 aatacctttc taaaaattat ttttttaatt ttatgaattt aattatactt tttctagtat
    15661 caggatttac aattaatata gaaccaatca taattggtgt tagtatacaa aataaaaaaa
    15721 taatatcttt ttttagatta tattctttag attctaattt ataattatta tttattattc
    15781 catcataatc acttatattt gacttatttt tatttgtatc ttgatttttg ttagaaatac
    15841 tttccctata aggaaaagaa tgttcattgg taccaaatac tgattttgag cttaaatcat
    15901 aattatcatc attttctgta attgaatttt tattgtaaaa tcctttttct ttatcagaat
    15961 tattttcttt attaccgtct ttaggtatat catgaggatt tttattttca ttgctatcag
    16021 tacttgattc tacatcatct ttaggagtat ttggttgttt ctcatcagaa ttatcatttt
    16081 cattattatt attttcatta ttattgtttt gattgttatt actatcatta ttttcatttc
    16141 cattattccc atcttgattg ttatcatcct catctctatc cgggtcgtct ctacctcctc
    16201 ctccacggtc accatcaata tcatcatttt cccgttcatc atctggcgtc tcttgattgt
    16261 ctccgcctcc cattataaat cctggtttaa aaactatatt tgaataccct tcttttaatc
    16321 ctcctaccac ttttactcta aaacaacgca acttaaaatc ttcaaaaaaa agtatagctt
    16381 gatctctaat atcctcctgt tcttcagata tccaattgat tccatcgctt gaatattcaa
    16441 caaaaacctt acttgcacta tacggcatat cataaaataa aacagctaca tatccatttt
    16501 ttgtattttg aaattccagt tccaaattgt ctattggttt tttctctata acagatacat
    16561 tcaaaatagg aactacatca tcatttatta taattttttc tttatttaac atttctgttt
    16621 taaactttcc tgttatatta aaattttctc ccttttccaa cttttcataa taatcagtaa
    16681 aatcccattc tacatctata tcaagtcgat taccactaga tgtttctaat accatatatt
    16741 tgggaaattt tatattttct attttatcat ctttacttac ttttatttca tcactaaata
    16801 caccaattcc cctatgacaa catttcacaa tatcatagcc agaatcttca gctatagaag
    16861 gtataagctc attataatca tcaattatat aaactttctt actttctgat ataattgatt
    16921 gtgccaactt attattatct cttagtaacc cactttcatt gttgctacta tatgctttta
    16981 ttgaagtttc ttctatctca acatcacctt tagaataaac acctatagca tgttctccat
    17041 tcacttcaat atccttattt agtattctaa tatcattttc agtataaatt cctattgcat
    17101 cttttccatt tgcctttatt ttttcaacag attcgtagga tgatttaata tgtaagtctc
    17161 ctccttcttc aacgtataag gctgtcccat cagtagcaat tatgttgttt ataccaatac
    17221 ttatacttcc accattttct atatgtatta ctccttcttt tcctccttca cctattatat
    17281 tcaattcatt atcatcaata ataaatctcc catgattttt tatcaatatt ttatatttgt
    17341 tggtatctat tgtaaaatta ctgtcataag gaatataaaa tctaaactct tcatctatta
    17401 ccatatcaca agaaagagta taaactattt cttctttatt atttttattt tcttgaaccc
    17461 acctttcaaa atcttcaaga caattttcct cttgtccaaa aactttatgt ggaaaactta
    17521 taaaacacaa aactattata ctcataaaaa taaatatctc atactttttc atcttacact
    17581 cctagaaatt tttagctttg aaacaatcct gtcgaaacat atacttcacc cactataatt
    17641 tttccacaat tatcaattat tgtcaattta tataagtatc tttattaatt gttataaata
    17701 aaaagctact tactaaagca aacagacgtt tcactttaat aagtagctat atttttataa
    17761 caaaatagtt ataatattag aacttccttc atttacaatt ttttgtaaag tcttttgcat
    17821 tttaactctt atctcttctg gcatagtata taatttactt tgtaattgct cttttactaa
    17881 gtcatgtagt gatttaccaa acatatttga ttcccataaa tctgctggtt gctcttcaaa
    17941 tacttccata agatatttta tcatctcttc accttgattt tgatttccaa ctattggaga
    18001 aacctctgta gatatatctg cttttataat atgaagtgat ggtgcattgg cttttaactt
    18061 gataccatat tgttttcctt gtttcattat ttctggttct tccagactaa gttcttctaa
    18121 ggaaggagct actactccat atccttttat cttggcatct attaaagcac tttctatttt
    18181 atcatactca tttttaactt tagatagtct agttatcaga ctaagcagtt gataatctcc
    18241 ttcaatttta aatccacttt tttcttcaag gacattatag aataaatctt gctttgtctg
    18301 taaatcaata tttataacac cttctcctag ctctacatta tcaacacctg tatcttccaa
    18361 aaactctaac tcagaaaatc cttgtataat accttctata tcccttattt tacctatatc
    18421 tataatactt tgttttaatg ttgttattat gctacttttt atccaatgat ttctttcaag
    18481 accttcaacc cattttggta agttgattct tatttctgta agtggaaaat catataatac
    18541 agcttccata acctcctcta tatcttcttc ttccatttca accacattca ttggaagaac
    18601 tggaacttca tacttttctt ccaattcact tctaagcata ctcgtttcct cactttttgg
    18661 acttaacgta tttaaaacca ctgcaaatgg ttttttaaga tttttcaatt cttgtataac
    18721 tctctcttct ggttctacat agctttttct gtcaattcca gtaactgaac catctgtcaa
    18781 gacaactatc cctattgtag aatggtcttt tataactttt ttagttccta tctctgctgc
    18841 cttttcaaat gtcatagctt cttgtgacca aggagtggat actaatcttt gttttccacc
    18901 ttcctcatgc cctaaagctc cttcaactat gtatccaaca caatctacca ttcttacttt
    18961 taaagatact gtgtctttta tttttatttc tacaccatct gctggtacaa attttggttc
    19021 cactgtcatt atcgttttac ctgaaccact ttggggtatc tcatctcttg ttctatcctt
    19081 tttaaattca ttgtctatat taggtataac caacttttcc ataaattttc ttataaaagt
    19141 tgattttcct gttcttacag gtccaactac tccaatatat atatccccct gagtcctttt
    19201 ggatatatct tcgtatatgt tattattcat attattcctc ctatacatct aatcttatta
    19261 aattatattt agagttaagg gttaatattc ttaaaataga aaaataaaga ccagcaatat
    19321 taatttaatt tacatataga aatttgtaaa tttcaatgta aattaaatta atattgctgg
    19381 tccaaatagt agtgtattat attaactgac tttagtagta ttttcatttg aattattatt
    19441 catctcaata agttctttac gtctttcttt tgtcatgttc atccatataa atgtaaacac
    19501 caatccaatt atggctacta tagtaagaac tatgaagtag tacctatatc cagttacacc
    19561 cggaaatgca tccaatatct tccctgctgc taaaggacaa agtacttctg gaagatatcc
    19621 taatgtagca actattccta tagcagttcc tgatattgat aatgaataat taccttcttc
    19681 aagtaacgaa tagtgtaaac cttgaattgc atacatagat acatatattg caacacatcc
    19741 aatcaatagt atataaatca tagatgattt tgttggtgta aatattacac ctaaaagacc
    19801 tgcaatcata agtataaatc cataagatat aatctttgat gaaccaactt tatctccaag
    19861 taatccagaa gcagctgatg ctactggtct acaatactga gctaatattg agattgcaga
    19921 tgcaaacacc acagtagctc caaatgcttc agttgaataa ggcgtaaaat aataaaatga
    19981 catattagct gaatatgaac aaaacattat tattgttatt atccatgtat gaggcatttt
    20041 tattactgca aaaaaggctt ctttatcaaa ttttgctgat acctcttttt cattatcctt
    20101 taatttgaaa aatactaata ctccaactat tacatttatt gccgagtata atactataat
    20161 agctgtaaga ccagatttat cacttacttt tgatgaaata tacccaaata gtatcaatac
    20221 tagagataga tgaattgcat tggtaattcc acgaccactt tccataaatc caaaagcttt
    20281 tccttgttca ttttcagatg ctaacattct aacagctttt acaagagctg gccaaaaagt
    20341 aagtattgtt gttattcccc ataaggcatg gattaaaaac acaacaatat atggaggata
    20401 aagtgttaat acaagaccag atattcctgt aataattaaa gaacctgaaa gcaattttct
    20461 ggctgaaata cggtctgcta aaaaaccacc aaaaaggtat gataccattg caagtagacc
    20521 ataaatactt cctaagcttc ccatttgtgt atttgtaaga ttaaatgctt gtgcaaaagt
    20581 atcataataa taattcttta aatatggtaa agcatatata aatgctcctg ctacaaagag
    20641 tattatcaag actttgaaat ttttccaaat gcttggttta tttacttcaa ctgacatact
    20701 tcctccttat gtaaatacct gtaaattttc atgataatta attattgctt attgtattgt
    20761 ttattcaata tttcggaata tttattaata tattaggtta atctatcaat aatttatggt
    20821 gagttatgag ctaaaagtaa actcttttag ctcaccataa taattatatt tttatttata
    20881 ctaacgtcca gagttattag atatcttttt ttcatagttc ttccatagca ttaaacctgt
    20941 tattataaca aaaagacctc ctcctatcat ttcaatctca tagattatat tatcttttaa
    21001 agtctccata actggtgccg ttgagaaaag tatgccaact acagttacta caagtaccat
    21061 tctggcaata ttgatacaaa ctgaatcact ttttgcaatt gaatatggtc tttcttcatt
    21121 tggtcttgtc tttctaagtc taatataaga tgtaaaaagc aagacatatg gaaataatgc
    21181 tgttaatgct gtcatagtaa caagaacatt gtatatatta tctactgatg gtaataagtt
    21241 tgttcctaca agaagtagac tcactattat agcctgaact attactgcat ttgatggtat
    21301 attgtattta tttgtaacag tcaattgctt aggaaataca ccttctctta catttccaaa
    21361 aagcatcttt actggacctg ctatataaag tattaatgct cctagtactg ataaagttat
    21421 tccaaatgct actattctta taaaccaact tcctatacct aaatcttgtg caactttagc
    21481 taatgcatct agtatccctg tagatgctgc aattttatct ggaggaagta tcattgttat
    21541 tgctactgaa cctaagatat agattcctcc tattaaaaca gccacagtta atatagcttt
    21601 aggaaagttt ttcttagcat tatccatttc ggttatgaaa ttagcagtcg tttcagctcc
    21661 tgataatgca aatattatag cagatataga aactaatgaa ttggcatcta tttttggtat
    21721 tatattttgg attgtatata tcgatgctga tggagctttt ttgagaataa ctacagacat
    21781 aaatgccatc actataagta atatagcagg aattgtagaa cctaatgctc ctgtattcgt
    21841 aaaaaatttt ccaaatgcca tcccacgagt acttactaag gacaacagcc aaaatattat
    21901 gagtgaaagt gctaaaataa acatcttatt gtttgataaa tctggatttc caagcatata
    21961 tgcaatattt atagctaaaa atgtcaagaa agaagaatac caaaatatct ttgatgtcca
    22021 atttagccat gacaccataa atccccactt ctctccataa gcttgtttaa cccattctcc
    22081 aagtccacca tcttttggat atgttgcagc caactctgca catataagtg atgcaggaac
    22141 aaagaatata aaagcaaaca ataaccaaac tggtatagaa ccaagaccca atccaaaact
    22201 agaggcagta gatttagcta tccatctaat cccaaatagt gctgatatat tcataaatac
    22261 taaatcccaa aatccaattt tctttttcac tgtaattcct ccaatttcaa atttattcaa
    22321 actcatacac tatgtattga tttaaatatc aagtataagt tcttcaacac ttcttggata
    22381 attagttata attgtagcac cattcttacc tactattgct gtatctgagt gtctaaatcc
    22441 tccaactcca ggtatatata tccctggttc catagaaaat accattcctt cttctaatat
    22501 taattcattg tcaaatctta aatatggctc ttcatgttct gacaaaccta aaccatgacc
    22561 aattctatga tttgaatata actctagacc atactttgca acaacagctc ttgctgcttc
    22621 atccaccatt cttgctggta taccagcttt tattgtatct aatccagctt gttgagcctc
    22681 tactgcaatc ttaaacactt ctttttgtct ttctgttggt ttgcctataa taaaagttct
    22741 ttcattttct gctctatagt tttcatacca aacctgtcta ctatgtatta ctatatctcc
    22801 tctttgaagt attctagtat tagaatctaa atgaggttgt gctgttctat caatccctga
    22861 acaagtccag tttgcaaatc ctatatatgt atcaggataa ttttcagaaa catattttag
    22921 taaagcatta tccccttcaa cgtcaaactc caattcactc atcccaactc taacattttc
    22981 taaagaccct tttattccta tatcagatag gtaacctgct atcttcaaaa attctatttc
    23041 ttttgcatct tttacatatc tcatttcaaa tattttactt ccaacatctt tgatagcaaa
    23101 tgacttatca gttatatact ctgaaaagct tgcagatact atatcttttt ctattccaac
    23161 agttgttcct ttagggtatt tatttaaaat tatatctaag taatgtttat gtgatattcc
    23221 atgttctttc atttctggta tttcataata cacatataca ttatcaactt ttgcaacttc
    23281 tttagcatgt aattcttcta atccaggtac tattaattct attttatctt gagttactac
    23341 tgttacaatt ggtctagaat aagttattgc tttaaatcca ctaatataaa attgattgtc
    23401 tggttcaaat attaaagcaa tttcaatgct tttttcattc atgtactttc tcaatctttc
    23461 tagtctcata ttatttgaca taatgcacct ctttcttaat attcagataa tttattcaat
    23521 acaacaagga tgaatatatt attttaaaat tcttaatatt tttagtactt gtcgtatttg
    23581 tatgtaaatt aattttattt gtgattattt tatcataatc atgattattt tgtcaatcat
    23641 gattttattt taaaaaattt tttatattct agtgatttta aattatctaa aatataacga
    23701 gtatacattg tattttgtaa tattattctt gatttatggt aagtttaatg ataaaatcac
    23761 gattatataa actatattga ttatttgtag gtgaataaaa tgacaaataa agaattacaa
    23821 aagaaaagga tattgatgta tttcatagaa gctgctcaaa atattatgga aaacgaaggt
    23881 atagaaaata ttacacttcg taaggttgca gatatggctg gctataatag ttcaactcta
    23941 tataaatact ttaaaaattt agaccattta atatcatttg catctataaa atattttaaa
    24001 gattataact tgaatatttc aagatgtata gaaaatgtca atgatgaata taagaaatat
    24061 ataattatgt ggaaattatt ttgtaagcat tcttttgata atgctcaagc tttttaccag
    24121 gtatttttta acttgtctag tgatgaactt agctatataa caaaacaata ttatgatatg
    24181 tttccagaag atttaggtat tcatgatagt gacatatctt taatgattac aggagaaagt
    24241 ataaaggaaa gaaataagat actattaaac aatcttgtag atactggata tataccagct
    24301 aaagatttag acataatcaa tgatataatt atttcttttt atcaaaatct attattctta
    24361 aaaaagaaca caggaaaaaa ttctgatgat gatgaattaa ctatgaaaat gatttcttct
    24421 attgaataca taataaaaca tgctaaataa aattaaaata tggctgtctc aaaatagaga
    24481 taagcctttt ttattgttga aataataaaa atatatttaa tttcatataa aaatgagtgc
    24541 ctcgtaaact aaaaagttca ttttgagaca cccctaaaat aaatttatta tttattatac
    24601 aatagtttat atatcatcac ctaaaactac ttcttccatt tcatgctttt tacttctcat
    24661 cattagttct attccaactt ctttaggatt tgaacctttc tttattacag aatatatagc
    24721 attagttata ggcatatcta tatctaattt ttcagcaaca tcatgtgcta cttcagtagc
    24781 agttatgcct tctacaacca tctttacttc ttttaaagta tcttctaaac tcatcccttt
    24841 acctatgagt ataccagctc ttctatttct actatgcata cttgtacaag taactattaa
    24901 atctccaata cctgaaagac ctgcaaaggt agacatatta gctcccatag caattccaag
    24961 tctgctcatt tcactaatac ctcttgtcat aagggcagct tttgcattat caccataccc
    25021 taatccgtca catatcccag ctccaaatgc tataatattt tttaatgctc ctccaagttc
    25081 tactccaact atgtctggat tcgtataaac tctaagttta gggctcataa acaaatcttg
    25141 tatcatctgt gctattttta aatctttaga agcaactact acggtagtag gaatatccct
    25201 tgctacttct tcagcatgag atggcccaga tagtattaca tatggatttt gtggtaattc
    25261 ttcttcacag acttgtgata atctaagccc agtccctttt tcaagacctt tagctacatt
    25321 aacaataact tgtccttctt ttataaatgg ttttattttt tggcaagtac ttcttacagc
    25381 ctgtgatgga actgctaaaa ctattacctt acaatctttt atagtttttt ctatatcagt
    25441 acttatagtt atattatttg gaaataatac tcctggtaaa tagtctgtat tttctttagt
    25501 tctgtttatt tttttagctt gttcctcttt tcgagtccac atgctcacat cattaccttt
    25561 ttttgcaagc cctaaagcta atgcacttcc ccagcttcca gtacctaata cacatacttt
    25621 ctccatgttt aacaccacct gagtataata tatttactat tctttctttt gaccaatttt
    25681 tctctctgtc ccatttaata atcgctttat atttgctctg tgattatata ttactgatgc
    25741 agttaagaac agagctacta ttaaaccagc tttatttttt accataatca taaatattgg
    25801 tgaacatcct attcctacaa cagaacccaa tgaaacatac tttgttatag ctaccaccaa
    25861 tataaatact gctagacaca ttagtgttat tactggattt acagctagca ttgcccctag
    25921 tgaagttgca acacctttgc ctcctctaaa tcctaaaacc gctggccaat tatgacctgc
    25981 tacaacacat ataactgcca gatatccagc taaaagagta tctattccaa ccaatcttgc
    26041 tgcaaattct gatattaaaa cagccattac acctttaaga acatcaccta aaaatgtcat
    26101 tgctcctgct ctttttccaa gtgtcctaag tacatttgtc gaacctgcat taccagaacc
    26161 ttgagtcctt atatcaaccc cagcaattcg ctttgcaact atgtatgagg tagatatatt
    26221 tcctaaaaga tatgcaacta cagcaataat tatataacta aatatctcca ctgaatttcc
    26281 cccctttagt tatattttaa gatatttatt ttttattttt ttgtctatac tcaaacttta
    26341 tggaagttcc ttcaaatcca aaattttctc ttatcttatt ctcaaggtat ctttgatatg
    26401 agaagtgagt taaatcctta tcatttataa acaatgtaat tttaggaggt ctaatgtcag
    26461 tttgagtccc atagtaaatt tttaatcttc tacctttgtc agatggtggt tgatttaaca
    26521 ttacagcttc tccaattact tcattcagtg ctgaagttga tattctctta gaatgttcat
    26581 ttgacacata ttcaactgta tctagtatct tgttcattct ttgattagtt tttgcagaaa
    26641 caaatacgat tggagcatac atcataaatg gaaatttttc ttttatatct tttgtgtaat
    26701 tactcatagt cttattatct ttctctatta aatcccattt attgattaca aatatgcatc
    26761 cttttccttc atcatgtgct atacctgcaa cctttgtatc ttgttctgtt acaccttcta
    26821 aggcatctat tactatcaat actacatctg ctctatctac agcactcata gctcttatta
    26881 cactgtattt ttctattgtc tcatatattt tactttttct tctaagccct gctgtatcta
    26941 ttagaagaaa tttctgtcca tttttttcaa aataagtatc aattgcgtct ctagtagttc
    27001 ctgcaattgg acttacaatt actctttctt ctccaagtat cttattgagt atagaacttt
    27061 ttcctgcatt tggtttacct gttatagcta ctcttattat atcttcttca tactctgtat
    27121 ctaatcctgc tggaaaattt tcaactatct catctaataa atctccaaat cccatactat
    27181 tggctcctga aactgcaaaa ggcgttccaa atcccaactc ataaaaatcg tatatattat
    27241 caaattgact ttgactatca atcttattaa ccactaagat tactggtttt tttgtctttc
    27301 taagcaattg tgcaatttct ttatcagcag ctgttattcc agctttacca tcaactataa
    27361 aaagtattac atgtgacatg tccattgcaa gcatggcttg atttctcatt tgagataata
    27421 ttatatcttc gctatcaggt tcaattcctc ctgtatccac taatgtaaaa tatttatcta
    27481 accattctac ttcagcaaat attctatctc ttgtaactcc aggtgtattt tcaactattg
    27541 atatcctttt tcctgcaaat ttattaaata ttgtagactt accaacgttt ggtctcccaa
    27601 ctacagctac aactggccta cttatactca ttttatttct ccttccatct tttgttttct
    27661 attttaaaat tttatccaca aaatcttttc cttcattcaa acaagggata atttttattc
    27721 tcataagttc ttctaattga tttaattcaa tattatctaa gaaaatttct tcatctgctt
    27781 ttagcatact tcttgttata taaagagctt ctcctaattc tttatcttca agttgttctt
    27841 ttaaatcctt agctgttaaa agacctgata cagttattgt ctctccaaaa aacttgtttt
    27901 tgatttcata tacatttact tgaagatttt taaacttgtc catcatagca tctgccatac
    27961 tctgtataaa ttcatatgca gaatgaccag tagctattga tactttcttc tctcttttta
    28021 gaatcttctc aggcaatata tttagatact cttcaatctc tgtttttaac ttacgtatca
    28081 tacctacacc atcttcaaac tgtaaaaaac cttcatattc ttcatatcca ggcaattcac
    28141 tattagatag tatataaaat tcatctgata aaaatgcaaa tcgagtacct aatttttcaa
    28201 gatatttctg ttgtatttga tgtatttgct ctatagtttt acttgcgctc ttatcattaa
    28261 atatatttaa ttctactaga ttttctctat gctttgtaat cccaactgga actatcgcaa
    28321 cactgtttac atacggataa agttgagcta gttcttttat tgttctatcc aattcctttc
    28381 catcatttat acctggacat aaaacaattt gacagttcat ttctatatga gtatctgcca
    28441 atctcttcat tataccatat aattttcctg caaatttatt gcttatcatt ttttgtctta
    28501 actctggatt agttgtatgc acagatatat ttataggact tattctatat tttataatat
    28561 tatttatatc ttcttcactc atatttgtaa gtgtgacaaa atttccttgt aaaaaagata
    28621 atctcgaatc atcatcttta aaataaagag tttctctcat accttctggc aattggtcta
    28681 taaaacaaaa catacactta tttctacagc ttttagcttg gtcaattatc ggatttgtaa
    28741 attcaacacc caaatcttca tcatagtctt tttctatctc gtatatgtag acttcgccat
    28801 cttttttttg tatttctaca tctaaatatt catcacttag taagaatcta tactctatta
    28861 tatcatgtat tggctgttca ttcacagata taagtaaatc tcctacctct atacctattt
    28921 cttctgcaat actatcttta taaaccttac ttataatatt atttactttt tttacattta
    28981 cttccaatat tttcaccact cttttgtatt catcaatgct tattataata tattttaata
    29041 tgcaatttat ttatttatta tattttatga tagcattttt aagcatttca tgtcataata
    29101 acacatttta attctaatag tcaatataag taaatagcta taaaatagat tagaacaaat
    29161 aatattcaaa tttaagtttc ttaaaaattt gaatattatt tgttcttaaa aaaattttat
    29221 aaatttaatg ttttactatg attaacgtcc catttgtcat atcatgaacc attccaccta
    29281 cacactttga catcaacaat gccttatttt gtaagtcttt ctcatcattt gcataaaata
    29341 ttggacaacc tccaccattt ttcatattct tatcaagtgt aactatagct agggtatatt
    29401 catttaaacc tatatccatt cccatcttat gcacttcctt tctttcctat tttataattt
    29461 tttagtgata agttttttcc tttagaactt gaaagtattg ggcatgactt tacggcttcc
    29521 attacttttt ccacattctt atctactggt aaataagcaa tcattatact ttcatcctct
    29581 ttattccttc taggtaatgg tacgaatgaa ggttctccat aatctccata taaacctaac
    29641 cttgaatata tattgtatac tattgcctgt ctttgacctg agtcaaataa tatccctgca
    29701 ttagcataat ctttattttt aggaattatt tgtattccta cacctctatt aatatatact
    29761 tctctatcac tttttagacc tatatttgtt attcccttta aatctcctac cttcaatata
    29821 gactcatcta caaaactaat ctcagcaggc attacatcag ctatgtctcc aatacttttt
    29881 ctagtaagaa gtttttttaa tataaaggct aacactaatc cacaaattga acttattacc
    29941 aaactcattc cttcacttat tttcgtttct gcaagtatta cataatacaa tcctattgtt
    30001 aaaaaagatg taactataca catataattt cttacttcat aggttctagc tatctcttct
    30061 atgaatgcag ctcctctctt tacaagttga acttcctcta agttttgcaa tgtatttctt
    30121 ctatttgctc tcacttgtct aaattgctct gctgctaggg ataaaaaagt tatagatgta
    30181 taagaacgat ttataagagc tggaaccaat aatgctccta atgaagaagc gacgaatgct
    30241 aatataagtt gagatagtaa aacatttggt tgcgttggat actgtttttg gtctaaattc
    30301 aacattataa ttctagatgt tataccaata ataacagcaa caaaaaatac tctatccatt
    30361 aaatcactcc taaaatattt aatttgaaat tttacatgta aatctacaaa gtctttctag
    30421 aaaaatcact ctgctttcct tttgatgttt ctatgatagg tgtacttttc actacatcta
    30481 tcatgacatc tatatctttg agtattggca tatatggtat aaccacagta cttttttcta
    30541 aattggtctt agatatagca acaatatcaa cctcatctac atctctatct atccccatat
    30601 gaataaatag attatgtatt atagcttgtc tttgccctat atcatttact attccaaatg
    30661 cacctaaatc tcttggtata acttctatgg ccagacattc atttctatac ttttctcttg
    30721 tactttctag acctatgtta gttattatta caccatttac cattaaaata ggtccttcaa
    30781 aacttatttt tgcaggaact atatctgcta tatctgctat actgttcctt cttaaaaatc
    30841 ttctaaaaat caaaccaaca atagctcctg acacaatagc taggattgtg caataaaaaa
    30901 agctaaggcc atactttctt gcaaaaacga tataaactat cgaagatacc agtgcagaaa
    30961 aaagacttat ataacttcta gattcatacg tagatgctat ttcttcaacg taagcgtctc
    31021 cttttggtac taattcttca ttatcgatat tctttagtgt tattctttct tgttctgcaa
    31081 gaccttgaaa ctgttgtatt gctactgcaa aaaatgtcaa ggctgcaaac tccttatcta
    31141 taagtgcagg taatgctata gcacctaacg aagctgaaag cccagatata attatctgtt
    31201 ctaaataatc ttgtggtctt gatgggtatt gtttgtctgt aactcttaaa actaatcctc
    31261 tacataatat tcctacaact aatgcaacta taaaagagtg tctaaataaa tttgcattca
    31321 ataaactttc ttccattttc tcagctcctt ctcttatata gtttgtgcaa aaaacaaaaa
    31381 aaattccctt ctatttaagg aaattttttt gtaatattgt aactataaat tcgctaccaa
    31441 tgccaagttc actttttact tttatacatc catttagtga tttgactata tgctttgtta
    31501 tagccaatcc taaacccgtt cctcctacat ccctacttct tgctttatct accctataaa
    31561 atcgttcaaa tatgcgttct acatcttctt ttggtatccc tacaccattg tctataactt
    31621 taattacgat tttttcttca tcttttaaca ctacaacttg tacaatccca ttatcttggg
    31681 tatactttat agcattatca actaggttta agaacacttg ctttatatag tctttattgg
    31741 atagtatact tattgattta tcatcacatt tatataaaac ttctatattt ttttcactag
    31801 ctgaatgttg agttatttca tatacctctt taaatacatc atatactaca acattttcca
    31861 taagaagagt atctttattt tctacaaatg ataacagcaa tatatcatca atcaaccttt
    31921 ttaatctatc tgattcgctt tctattattt ctaagaatct atttctagta ttcacatcta
    31981 tattttcatt tgactttagt gtttcaacaa acccacttat agatgtaagt ggtgttttaa
    32041 gttcatgact tacatttgca acaaaatcac ttctcatatt ttcaagttta acttttttag
    32101 taatatcttc tatatttact atcgaaccta ttattacgtt gttagtattc tgaagataaa
    32161 ctgggtctac ttttatctta tatacgatat catctttagt agtaatatta gcagtttcat
    32221 tatctttaga accaataaat tgaagtattt gttttaatat ttcttcttct tttattacaa
    32281 cattaatatt atgaccttca actaattgat ttggttcact ctttatcatt tcttttgctt
    32341 catcatttat cagaagtata tttccattta catctatagc taaaattcca tgagatatac
    32401 tctttaatat agaccttagc tgtaaatgtt tatactgtac ttcttctaag gttttatcca
    32461 tattctttat catatgatta aaatttctag ctaattcgcc aagttctcct ttggctgata
    32521 tgttaagcct agcatgatat tcattattac ttactttttt tgagacgtat ataaactctt
    32581 ttaaatattt ccttaatgtt attgtgtatc ttacagctaa tataacaatg gctatggaca
    32641 acataacaat aaaaaaatat atactattta aatcttccac aagataacac tcctaaaata
    32701 taaccttttt aatctatttt gtatcctaca cctcttatag tctctatata tttttccgat
    32761 ttatcttcat cttctatttt ttttcttaaa tatcttatat gaacatctac tgttctagtt
    32821 tctccatagt attcataccc ccatatttta tccaaaagat aatttcttga aagtactttt
    32881 cctttatttt gaattaataa ttttaacaat tcaaattcct taagagttaa ctctatcttt
    32941 tcagagcctt tggtaacttc atgttttgat aaatctagtt ttaaattacc tgtagtaagt
    33001 atattgtttt cttcacctaa gctagtcaaa ttatatctcc ttaaaacaga actaatccta
    33061 gctaacaact cttttatact aaatggctta gttatatagt catcagcacc tatttcaagc
    33121 ccttcaacct tgtcattttc catattttta gcagtaagca ttataacagg aatatttttt
    33181 aagtccttat cacttcttat ttttctcaaa acttctattc cacttatatt tggtaacatc
    33241 caatctaaaa gtattaaatc tggtttaatt tccttagcct ttataaatcc gtcaaatcca
    33301 tcatatgaat aactaacttc gtaatttgac acctccaaat tgaattttaa tagctcaact
    33361 atatgcattt catcgtctat aacaagcact ttagtattca ttaaaaagct cctctctatt
    33421 ttatctgtag taccgttcct attcgttctg atgccttatt atgttttcta taagagtgga
    33481 atttatctgc tctacaacta gtgcataaat ttaaatttat tatattttct tttttaactc
    33541 cactacatct aagcatatac tcattaattt tccataaatc taaatagtaa cttccctctt
    33601 ttattatata gaacttttca tcattatttg taagaattgt gttaaatttt tctactaaat
    33661 cttcagacac ttcataacaa catggtccaa ttgatggccc aattatacat actaaatctt
    33721 caggaactgt tttatagcat tcagacatta attttattgt tttatgacct atattactaa
    33781 aagtccctct ccaacctgca tgagccagtc ctatggcttt atttttagca tctataattg
    33841 atataggtac acagtctgct gtaaacagta aaagtggtac tttttccaag tttgttataa
    33901 gtgcatctcc atctcttctt ttccctatag tatctttatt gactatatta actatatctg
    33961 aatgtatttg agaattacta gttagatttt caaatgtcaa atcacattct ttacatactt
    34021 ttaacatatc ttctctattt tttgcatcta tattcttaca tgttataact agatttacag
    34081 aatttaattt ttcatcaagt tcttcacaat taccacttaa attaacatac tctttaatat
    34141 cttgtacatt ttttatattt atataatctt tcatcatttg tctccttttt ctatcagtat
    34201 acttttaagt tcatttacaa aagtatttat atctttaaat tgtctataca cagaagcaaa
    34261 tcttacatag gcaacctcat ctaactcttt caatttatcc attaccatct caccgatttt
    34321 tttggtttca atttcttcta taaaacattt atttatttca ttttcaatat gtacaacaat
    34381 actttcaatc tcgtcaatag aaacaggtct tttttcacat gcttttagca atccatattt
    34441 tatcttttct ctatcaaaat attctctact attatccttt ttaatgacca ttaaaggagt
    34501 tgtttctatt ttctcatacg tagtaaacct ctttttacat gattcacact cacgtcttct
    34561 tctgatagat tttaaatctg tatgtctaga atctattacc tttgattctt tataattaca
    34621 ataaggacat tgcatctata ttcctccttt gtttaactat atctcaaact catctaagct
    34681 taattctgaa cctatattta ctattattgt atcacaacct attttaagta tatcattcca
    34741 aaatataact tcttcatccc ctcctcttga aaaaaaactt ctacttccat ctccaaaaat
    34801 ggtaaatgag actaccttac cttcgtgaat atccatgtct atgtctatta taaaccccat
    34861 cctttttcca ttttttacat ttataatttc tttttccatt atatcagaaa ctctaatcat
    34921 attgtactca ccctttcttt aaaaataaat atgtatcgaa gtaaaaaaaa atgacctata
    34981 taggtcattt aaattaaaat aatgtttaca gtccatggat tacttataca tattttctca
    35041 tattctttaa agcatttttt tcaattcttg atacttgtgc ttgagatata ccgatttcat
    35101 cagcaacttc tatctgagtt cttcctttgt aaaaccttaa atctaatacc aacttttctc
    35161 tactatttaa tttcttaata gcttctttaa gtgatatttc ttgcaaccaa ttttcatcag
    35221 tatctttttt atcttgaacc tgatccataa cgaatattgc atctccattg tcttgatata
    35281 ctgggtcaaa cagagatatt gggtcttgta tggcatcaag tgccattaca actgattcta
    35341 cttctaattc taattccttt gctatttctg atactgtagg ttctttagaa tttgttctaa
    35401 ttaatctttc tctaacttgt agtgccttat atgctatatc ttttaatgac ctacttactc
    35461 taattgggtt attgtctctt agataccttc taatttctcc tataatcata ggaacagcat
    35521 aagttgaaaa ccttacattt tggcttaaat caaagttatc tatcgctttt ataagaccaa
    35581 tacaacctat ttgaaataaa tcgtctatat tttctcctct gttgttaaac ttttttatta
    35641 cactcaagac taatcttaaa ttccctctta caaactgttg tctggcttct tcatcaccat
    35701 tttttatttg taataaaagt tccttcattt gcttattttt aagaactgga agttctgatg
    35761 tattaacacc acatatttca accttattaa cttgcatata ttttcagtcc tctctcaaaa
    35821 gtattcatct attgaaatta tttacatttc caattttttt atacttttta agaagaatta
    35881 atttagcatt atacaaattt tttcatttct ttttgcaacc ttgagattat tttcttttca
    35941 agtctagata tgtaggactg tgatattcca agcattccag caacttcttt ttgtgttttt
    36001 tcaatacctt tatcaataag accaaatctt aattccataa tttgcttttc tctgtcactt
    36061 aatctgtcaa gtgccataac caacaaatct ctatctattt cttcttcaat tattttgtat
    36121 atttcatcat tttctgtacc caatacatca gataataaaa gttcgtttcc atctaaatct
    36181 atatttaaag gttcatcaaa tgatacttct gttttctttt tattattttt tcttaagtac
    36241 attaatattt cattttctat acatctcgaa gcataagttg ccaattttat atttttattc
    36301 agtttaaaag tattaactgc ttttatcaaa cctatagttc ctattgaaat caaatcttct
    36361 acatctatac ctgtattttc aaattttctc gatatgtaga ctactaatct aagatttctt
    36421 tctattaaaa ttgactttac actttcatct gtttctagct tttgtaaaag ctccatttct
    36481 tcttctggtt tcaatggagg aggaagtata tttgcccctc ccatatagta tattcccttt
    36541 ggctttatta tttttaattt aatacctaac atagtaataa agcttattat tctttctttc
    36601 aatcgtaaca ttccttgtcc cccttaaatt ttcacctaaa atccaggcaa tatatttgga
    36661 ttcattattg ctccatattc tctatcatta aaatttgaaa taccaagtat tatatttcct
    36721 atcttttgct catctacttc tatatagtca gcttttaagc ctattattat gcttgtctta
    36781 ttacttccag catgtttata aggtattatt ctaactctac ctgaagtttt atcatctaaa
    36841 cttcctataa tttcttgtgc tttcttcata tctatatctt cataattaat atttaatagt
    36901 ttttctggaa taaatccttc taacacacta gacttaacta ttattacatc actttgactt
    36961 attgggtctt ttaacaaatt accactatct actaaagcct tacagcaaaa acttttttct
    37021 agtaagttta tatttatagt ctttgtaagc tccttaatgt acttaagcat tttcaaatct
    37081 ttataaatgt attttaataa ttcacaactc acataagtac atgcaattaa gaaagatatt
    37141 tttaagtgac ttattcctgt aaaataaatt ataaaataag tgcttccaga aataaaaatg
    37201 tttactagat aaaaaactaa cgctacatga acataatctt ttttgctctt acttctaaat
    37261 gaaattaaag ttattatact catgattatt attttaaatg gtacagtaaa tagtatattc
    37321 attgatggat acaaataagc aacagaatag gcacctccta aactagcccc caataactta
    37381 ttttttttag gattatatct tttagtaaga atagaagtac aacttataat aatataattt
    37441 attactaggt tttctataac atagtactca atatacacca tgtatccccc cttaaaacct
    37501 ttaaacatat tataagatag tatgtacaaa attttatgtc acttttgatg gtcgaatcca
    37561 aaacaaattc tatttaaaaa gaacaatgat atatgtctta tactttatgt cgtcaaaaga
    37621 tattttttta atgccaaaca aatagacttt tttgtagaat ccaatatatc taggtagaag
    37681 ttattcgctc tacttttggt atatttacaa aaaaagacta cataatttaa taatatatca
    37741 agtaaatctt ttacttatat aaaatcaaat tatatagtct gtaaattcaa atataaatta
    37801 ttgtgctagt aatccatttg catattttgc agattcattt agtgcttgtt ctggagttat
    37861 gtctccagct atcatcattt gaagttgttt ccataaatat tcatatatct ctactccatg
    37921 aggtccaact gtcaatggtc tatacatatg tttatctctt gtcaccattt cttcaaatct
    37981 tgggtctcct tggtattttt cacctttagc aactggagct ctaggtattt ctttgtggaa
    38041 agcttccatt gattctttgc ttagcatata ctcaagaagt ttaaatccaa gttctttatt
    38101 tttacatgct gacattaatg ttaaatcatc tactgaagca aaacttccaa ctgtcttatc
    38161 ttctaatgat gttacatatc cccagtttaa atccgggaat gatttatcaa atgtacttgt
    38221 agcagcagag gatagcatta tagtaaatgc agcttttcct ggtccaaata cactttcaat
    38281 catttcatta ctatcttttg ataaagcatc ttctggaata tactttttca tatcatttat
    38341 aaagtttgca gctttcaagc cttctgcatt attaaattta actgatttta aatcatcatt
    38401 atatatatct ccaccagctt gccataaata cggataccag ttccagttta agcttccaaa
    38461 tactttagaa ccccaacctt gtgcaagacc ccactggtcg atttttccat ctccatcagt
    38521 atcttttgtt gctttttcac agattctttt aaaatcatcc catgtttttg gaggtttttc
    38581 tcctaatttt tctagtatat ctttattgta gtacaatacg cctggattag cagcttctat
    38641 aggtaaacca taaataccac ccatcatttt tgcatcatct aagtatagat aattatctga
    38701 agttcctgat ttttctaaat aaggtgttaa atcttctacc gcacccattt ctataaattg
    38761 aggaaacatc tctgcataca tgtatcctat atctggacct tcacctgcac ttattgctgt
    38821 tgcatatttt tctgcgtagt tatcccatgg tactatctga aattcaattt cacagttatt
    38881 ttctttttca aatttttcaa atatcggctt ccatgcatct gcatcatggt ctgttaatgg
    38941 tgcagaccat acaactaatt tttctttacc ctctttgcct ttagaatctt ctttttttga
    39001 acatcctgca agaattgata ctgatagaat ccctaccata actaaagaaa gaaccttttt
    39061 tagtttaatc atgcttaatc ccctttcaga ctttaatttg tctttttcct caaatgttta
    39121 catttaattc agatttaata ttaacaaata agattactct tgtctaatca taaaaaacta
    39181 tactaactcc tataactata ttttttttct atacctatta gatttaattc atactaaaaa
    39241 cttacttttt aaattttatc aatatttaaa aattttttta agtctagcca caaactaact
    39301 atctaaattg agcaaaataa aaagaagtct attgacttct ttataagctt acatattatc
    39361 ttcttcttct taggaatgta ggtatctcca tgctctcatc atcatcttct tcaattatac
    39421 ttgccttttt aggaggctct attttttcct cagcaattac ttcttcttca atttctttta
    39481 cagcttgttt tactgttgta tttaatgaag aacgtatgct tgatttaggt ttagtatcta
    39541 aatctaaggc accattttgt aatccttcaa atcctgtcgc gattactgta atcattattt
    39601 catcacctaa atcttctttt atagaagctc caaatataac atttgcttct gggtcacagg
    39661 attccataac taaagttgat gcctcattaa cttcaaataa tcctaagtta ggaccacctg
    39721 taacattaag aagtactcct ttagctcctt gtattgatgt ttctagaagt ggagattgaa
    39781 tagcttgtct tgcagcatca attgctctag tttcaccact agcactacct atacccatat
    39841 gagcaagtcc cttgtctttc attatagtag taacatctgc aaagtccaag tttattaacc
    39901 cttcaactgc tataaggtct gaaattgatt gtataccttg ttttaacaca tcatctgcaa
    39961 ctgcaaaagc atctaacatt gatgtatttt tttgtactat ctgtaaaagt ctatcatttg
    40021 gtatcgttat aagagtatct acttttgatt taagttctgc tattccacct tcagcgttct
    40081 tcattcttat cttaccttca aatgcaaaag gtttggtaac tacacctact gtaagtatcc
    40141 ccatttcttt tgccaagcct gcaaccactg gagcagctcc tgtaccagta ccaccgccca
    40201 ttccggctgt tacaaagacc atatcagctc cttgaagtaa ctttactatt tcatctttac
    40261 tctcctcagc agctcttttc cctacttctg gatttgctcc tgctccaagt cctctagtta
    40321 atttttctcc tatttgaact ttatattcag cttttgatgt atataaagct tgtttatctg
    40381 tatttactga tataaattca acgcctttta gttgagcttc taccattcta ttgacagcat
    40441 tgtttccacc gccacctaca cctattactt taatttgtgc acattcttct aattctacgt
    40501 caaagtttag catcataaac ccccttctct catgattcaa agttatcgta tgtattaatt
    40561 atatcatttt cttcttttat tttgttaacc tttaagtaac atattccaca aaaacgtaaa
    40621 ttttcctttt taaatttctt acttatattg tcaaatatta ctctttaagt ataaaataat
    40681 ttacgtatat attaaatgct ctttaatttt tacgtttttt gttcataata ttactaataa
    40741 tatatcgtct aattatagat aagttattaa acaatctcat accaaaaact aatacagcaa
    40801 catagtaaag agaaactcct aacttttctc ctatataagt caataatata gccaatatag
    40861 cattacctat gaaaccagta ataaaaatta cattatcata gttatcttca aaattggatt
    40921 ttatcgctcc aaaaatagaa tccattattg caagaatagc tactgacata tatatggaat
    40981 aatccataga atatgtaaat ggaatatagt aacctactac tacaccaaat gctaatcctc
    41041 ctaaaaccca taacataaat cttcacctct ttattttact ttgaaacaca actacattac
    41101 aatccttttt attaattttt tttacttttt ctttattttt ttgtattttt cgttttattt
    41161 tgcaaataaa ttcactttat ttccattatt ttcctgaact tttatattaa ttccatatat
    41221 atccttaaga gatttagcat atgattctgg agagtttata gctgcactca ataaatctat
    41281 atctcctata gcttttatta taaatggttg tccatatgta gtatcattta ctgtaatagt
    41341 agctcctgaa cattttatct tacttgttgc tagtactctt tctccattta tagaaattgc
    41401 tcttgctcca gcctttttta aatcatttaa aaccctcaaa atatctatat catgtataat
    41461 caaatcgttt ggattctgtc cgtcttttaa ctctctctca ctatccctca tagtaatagt
    41521 tatgcctggc cctgtaacag ttgaataacc acttaattct ttaacttctt caagctcttt
    41581 ttgcataagt gtttggatag attcatttcc attctttttt gcttctttat attcattcaa
    41641 agtttttttg ttctttttaa gagtttcttt taagtttttt atttcttttt ttgtattttc
    41701 tacctcactt tcaatacttt cagtttgacc taatgttatg tatactctat ttgggtctag
    41761 tgattttata taagttgata tgaaaacgcc taatatgaat gctcctaata taactatttt
    41821 ctttttcata ctattatttc ccctctgctt tgtttatatc actatgacca tttatttcta
    41881 tattgtcttc tttggtcact tttactttgg cattataata tgtttctatt tcccagacaa
    41941 taccatactt tatattaagt gccgaagcca atgtatcaga atcaccaatt gcttttatta
    42001 taattggttc ttttatgaca gtgtcattca tataaagctt gtcttctttt aaatacatat
    42061 atgtattaga aactattctc tggtcgttta tagatatggc acttgcttgt gctgaattta
    42121 atttatttat cattgataat aataaatcat aattatatag tatactatca tttttatcag
    42181 agtttcctaa gtcttttgta gaatttatct caatttttat gcctgttcct tcaacatctg
    42241 tatatccagc taatgtttca tatttttcta tttctgactt aagtatgtct tgttctcctt
    42301 cattgttttt gcctctataa atctctatat ctttcttcac actattaact tctgattgta
    42361 gattctcttt ttcttttttt aaagatttaa gttcttctgt aagttgctct cctcttttaa
    42421 aagttgtcat ccctctattt tctatattaa taattttaag ttgtaaacta acaagaacac
    42481 ccaatacaat acttcctatg attatacttt tatttataaa tctttttttc aatatatcac
    42541 ttccttacaa ttgtctatcc ttctggacta tacactgcca actttccata gttaagattt
    42601 atagtccctc cattagtatt tttattttgc aggtcaatca gaattttact taccctagat
    42661 atattataat ctaagttatc atcattagaa agtaagattt ttatattact tcttgtaagc
    42721 atttctatat tgctttcttt ttttaagttt acataattta tttttctata aattccttct
    42781 tcttttaaat aatttaaagt tttaaataca ttctccttag ttttataatt tttaaatttt
    42841 atagatttat tatcatcaat gctataatct acatctacta tcaagtcttt tttactttca
    42901 ttagaacctc ttagttcttc caataaattt ccttttttat ctatataaca ataattatct
    42961 tcatctttta atactgcaaa tatttctttt tcttttaaac ttataattat cttattggga
    43021 agttttcttt ttatttccac attttctata tatggatttt ttataagttt atttttcata
    43081 tctttaaaat tgtatgcaaa aatattttca tttagattta catttagttc tttcattata
    43141 tttgatttgg tcaccctctt atttccaatt acatctattt tctttacatc aaataaatca
    43201 ctttttacaa ttatacatat acaaaaagat aacataagta taaaaactaa aactatcatg
    43261 acattattcg tatttaactt tcttcttttt ttcaaatata atcaacccct ttggttaata
    43321 aatacacctt tagagcttgt ttttcctaca aaaaaattat aatatttaaa gtcaaatttt
    43381 tgcattttat tacataacat ccaaataagg tattaaattt tataaataaa tcaatatttc
    43441 atcaaaataa acatagcata ctacatacta tttatatatt taaatctgta tacatacaaa
    43501 taaaccccta atttataata atttagaaaa tctaatatat tataaattag gggtaaatat
    43561 ttacttatct tttctttatt cctattacct tagcttgacc ttctatactt ggagtcgttt
    43621 ctttttttat ttcttttact tccttaataa ctttttcttt ttttgttttt ttagaagttg
    43681 acttttgtgt tgaattgtat actttcatta tctcatcata tataaggtct atggcttcag
    43741 gcttacctat agttttactt gcatttgcca tatcaaccaa aagttctcta tctcctaaaa
    43801 gtttaaatac tgctgtattt aggctctctg gagttagatt tttttctaaa atagcaattc
    43861 ctgccccttg tttttctata ctcttggcat tatactcttg atgattttca gcagtataag
    43921 ctttaggtat tattatagat ggctttccaa gagccgttat ctcagctaat gaaattgctc
    43981 ctgcgctacc tataaccaaa tcacttgctg caagtgcatt tgccatatct tctaagtaag
    44041 gcacaacttt ttggtatggt ttaagattta tatcgctaat acttcccata aattcatcat
    44101 aatagctttt gcctgtagca aatataaaag ctatatcttc atttaccata ttctttatta
    44161 caagtctcat tgcatcattt atttttcttg aacctccact accaccatag caaagtacca
    44221 ttcttttttc gtcacttatt gatagattct ttcttgcaat attttttctt gataataaaa
    44281 tttcttttct aactggattt cctgtaaaaa ctaatttatc ttctgctgcc tctggaaatc
    44341 ttttatgcga atcttcaaaa cttgtaagta cctttgtaac tgttttagat aaaatcttat
    44401 tagttacacc aggaaaagaa ttttgctcat gtattatggc tggtattttc cccatagatg
    44461 cattaaataa aacaggtcca ctaacatatc caccagttcc tataactata tctggtttaa
    44521 atttcttaac aatctttctt gattgctcca aacctttaaa aagcttaaaa actcttttta
    44581 cattatcgaa atctattttt cgcttaaagc cttgaactgt tacagtcttt aattcaaatc
    44641 catacttagg aactatttct gactctattc ccttttccgt cccaacaaaa attatctcag
    44701 cgtctggatg ctcatctctt attttgtttg caatagctat agctggatac acgtgccctc
    44761 cagttccacc gcctgataat aaaactttca tcaaatcatc atctcctgtt aattaatttt
    44821 gacatgcttt gagatgttta acactattcc tacagctccc ataaagatgg tgagtgaagt
    44881 tcctccataa cttataaatg gtagtgctac acctgtagct ggcattgatg atgtcgcaac
    44941 tgctatattt aaagctgctt gtattccaat ttgtgcacct atacctataa caaccataca
    45001 agcaaataca ttagaagtct tcaaagctat tcttacacat ctgtagacta aaactacaaa
    45061 tagcataata actattatac aaccaatcaa tcctaactct tctcctatta tagcaaatat
    45121 aaagtcattt tgtggctctg gtatataaaa atacttttgt ttactctttc ctaagcctag
    45181 accaaacaat ccacctgaac ctagtgcata tagaccttgt attacctgat accccttacc
    45241 aagagggtct tgaaaagggt ctaaaaatga tgtaacacga ctaagtctat agggttcagc
    45301 aattactaat gcagcaaata aagcagcccc actccctatc atagcaaata caattttcat
    45361 atccattcca gcaacaaata tcattacaaa agtaactaga attacagtac ctgctgttga
    45421 caaatttggc tgaagtatta tcaacgcaaa aaatatccct ggaactacga gaagtggtac
    45481 aacacctttt gtcaaagatt ttattttatc ataatttttt tcaattaatt ttgcagttaa
    45541 tattattgta gcaaactttg cgatttctgc aggttgaaat gttaaagctc ctattcccaa
    45601 ccatcttttt gctccgtttg cttctatccc taaaggggtc aaaactaaca ataataatac
    45661 tactgctata gctccaattg ctgtagcatt tttcttccaa aaactataat ctattcttga
    45721 tgtaataatc ataactataa atccaagtac agcatatatt acattctttt ttagaaaata
    45781 gtacgcatca ttgtgtttaa atgaagattg tataaaactt gcactaaaca ccataactat
    45841 tcctacaaat actaaaagca tagtagtgta aaacactact ccatcaaatt cagtcttcat
    45901 ccttatgtca atttgttttt tcaaattttc tttaggcatc caactaatct acctccattc
    45961 ttccatttaa gtaagtttag aggtaagaga ttattttaaa ttgtttacat tatctttgaa
    46021 gtcttttcct cttacttcaa aactttcata catatcccaa cttgcacaag ctggtgaaag
    46081 aagcactata tctccacttt ttgaaatttc ataagaagtc ttaactgctt cttccatatc
    46141 ttttactata tgtatgtcat taaatccttt atttttagca caattttcta tatttgatgc
    46201 agtttctcct aataagacca atgatttaac atatgacttt gcagtttcaa gaagttcatc
    46261 aaaattactt cccttatcca tcccccctgc tataagtatt ataggtctat cataagactg
    46321 tatagctttt atagtggaat ctggattagt tccctttgaa tcatttacat atattacatt
    46381 gtctaaattt cttacaaact cttgtctgtg ttccactcct ttaaatgtca taagtacatc
    46441 ctttataact tctaaatcaa ttttgcagac atatgctatt gctatagctg ccatacaatt
    46501 ttctaagtta tgacctccag gtaaacttac atcatcttta tttagaaatt tgattttatc
    46561 atctatatct attattatgt cattattttc taaatatact cctccattta cttttttagt
    46621 tcttgaaaag taaattactt tggcattgca tttatcagct aaagatttta ctatctcatc
    46681 atcatagttt aatacacaga aatcctcaac agtttggttc ataaatatat ttgcctttgc
    46741 ttctatgtat ttttccatag tatggtgtct atttaaatgg tcctctgtaa tatttaatat
    46801 tgcgcttact ttaggtctaa attcatctat actctcaagc tgaaaactgc ttagttcagt
    46861 tactaacaca gactcttcac ttgaagtttc tatagtatct attacaggat ttcctatatt
    46921 accaactaca tatgtatctc gttttgctct tgaaaaaatt tctcctacca aactagttgt
    46981 agttgtcttt ccatttgtac ctgtaatccc tataaaaatt ggattattcg atagtttaaa
    47041 agctaattct acctcaccaa ttatatactt gcctgaattt tttaatttca atatgaatgg
    47101 taggtctagt ggtacacctg gagacactac aaccatatct atatcatcta catcttctgg
    47161 gtgatgtcct aatatgtatt ttatgtcatt tatagatttc aattcatcaa gtatatttct
    47221 caatttattt tcatctttta tatcatttac gattacactt gcacccaatt tatctaaatg
    47281 tttaattgta gatataccag tttttgcaag tcctactaat aaaacttttt ttccttttaa
    47341 gtccattttt cattccccct agccctaaaa tactgctatt atacttatcc aagctaaaac
    47401 aactgttata atccaaaaaa taaatacaac tcttgtttct ggccaaccac actgttcaaa
    47461 gtggtggtgt attggagcca tcttaaagaa tctctttctt gttgccttaa aatatccaac
    47521 ttgaatcaaa actgatagag cctccgcaaa ataaattcct ccaattatag gtattataag
    47581 cactgagtta gttagaactg aaaatgctac aactgcacct cctaatgcca ttgaacctgt
    47641 gtcgcccata aacactctag ccggatatga attaaatcct aagaaaccta aacatgctcc
    47701 aacagttgct gctgctaaaa ctgcgacttc tgtatttcca gcaatagaac ttgcaaatag
    47761 cataaagaat acagaaacta ttaatgttac tcctgatgct aatccatcta atccatcagt
    47821 caagtttaca gcatttacta ttgctacgat tataaacatc attattggta tatataatat
    47881 tccaacattt atcacaaaat ctgtaaatgg tatcataagt tgtgaagcac ttgatgatga
    47941 tgtatactga taaaaagcaa cataaaaaga taatgcaact tgaaggatta ttttttgata
    48001 tgcttttagt ccaagtgatc tttttagctt tatttttata aaatcatcta aaaagccaat
    48061 aaaaccaaaa cctgcaatac atataagacc tactgccata tcatgactaa cttttactct
    48121 tgtaagtcca gttattagaa ttgcaacaat cattataatt ccacccatag taggtgttcc
    48181 gtttttagct agatgtgttt ggggtccatc atctctaact gtctgaccaa atttaaattt
    48241 tctaagcatt ggtataaata ttggtcctat tataattact attaaaaacg caatcaatgc
    48301 ggtgtacgta agctctgtta ttcctaacat cataaaactc ctctcccttg tcaattaact
    48361 cattagttta agcttattta tttagatatt cgactatttt ttctaatctc atgcctcttg
    48421 agccttttac taaaatagtg tctcctgttt ttactaattc atttaacttg ttaaacacat
    48481 cttctctatt ctcaaagtga tatatattgg ctgaattgaa tcctaattgt tttgcttctt
    48541 ctccaataaa cactgaattt tcacctatag ttattataat atcagtatta ttcatagatg
    48601 attttccaac taatctatga ccatactctg aaatttcacc catttctagt atatctccaa
    48661 gtatcgctac ccttctacct ttatatcttc caagtatctt taaagcagca tccatagaat
    48721 ctggactagc attatatgaa tcattaatta ttgttaattt ttcatttttt attatgtcta
    48781 atctcatttt tgtcccttta aaatttttta aacctttttt tatataatct aaagaaatat
    48841 ttagacacaa accaactaat atagcagaca ttgcattata tatattatgt tctcctattg
    48901 ttggtataaa tatttcctct tttttcccat ttattacaca tataaatgtt aaactatcct
    48961 cttccatagt ataactttca caataaacat cattgttttt attaaagcca aatgttttta
    49021 acttatatac atgttctttt tcttttaatg tagataaaaa cttatcatct ccatttacaa
    49081 ttaaagtatt tgtttcatca aagtttgaag taatctccat ctttgctttc aaaataccct
    49141 cttgtgaacc tagtttttct acatgtgata atcctatatt cgatatgact gcaattttag
    49201 gatttactat atccactaaa tattttattt cattgaatcc agacataccc atctctatta
    49261 cagcacactc atgttcttta tttagattaa ataaagtcaa tggaactcca aaatgattat
    49321 ttaagttttt ttcatttttt agtatattta actttgcaga tatggcagag taaatcatat
    49381 ctctagtagt agtttttccc acactacctg taacacctat aaatggaata tcaaattttt
    49441 ctttatagta ttttgaaata tctcctagag ccaaagatgt atcttttacc tctattaaat
    49501 ttatatccga actttctaat tttattccat tactttcatt ttttataaaa gttttacatc
    49561 cactatcata tgcagacttc ataaatgtat gcccatctaa attttctcca actatggcta
    49621 caaatgcatt ttgggcattt gcctccctgc tatctataac tatatcactt acacagtcat
    49681 tataatctcc atatattaac ttcccattag ttgcaagtac taactctttt attgttaagc
    49741 actccattat taaactcctc ctttaaaacc ttagctatat tctcttagca acaaaaggca
    49801 atgtataccg ttatatatac attgcctacg ataactctta actttataat tatatactat
    49861 atttctgttt ttttgaagat gtttatgggt atatgttaat tatctgttac tttttagttt
    49921 tcaaaatata gggttatact tgagtcttca ttaactttta ctccaggttt tgggaatata
    49981 tcctttacct ttgtatcttc tggaagttct atatctgcat ctaagtctgg ttttaacttc
    50041 gcatcctcca atgcctttac agcatctcct atttttagat ttcttacatc tggcacttta
    50101 acttgcttct tttcatactc agctttttct tcttctttat atactggttt aactcccaaa
    50161 tattttaaag agtcattcat tatttctttc actattggtc ctgctgtagt actaccaaat
    50221 gcacttactc ctgttggttc gtcaacaata gcaagaacta ctatttgcgg gtcatcacaa
    50281 ggtgctattc caacaaatga acatatatat tttcctggag catacttacc atctataact
    50341 ttttgagctg tacctgtttt accaccaagt ctataacctg gtatatatgc tatctttcct
    50401 ccaccttcag ttacaactga ttctgcaatt tctaacattt ttttagatgt ttctttagat
    50461 ataacacttc ttactttttt tggttttact gtttcagtta tatttccttt attgtcagta
    50521 taagatttta ccactcttgg ttgcattaaa tctccaccat tagctattga tgatatagct
    50581 gttataagtt gtattggagt tactgatata gattgaccaa atgatattgt agccaattca
    50641 actggtccta catttttttc gttatacagt attcccttag cttctcctgg taaatctata
    50701 cctgtcttat ccataagacc aaatgattct atataatcat acatttttcc aacacctaat
    50761 ctgcttccca actcaacaaa tactgggttg caagagtttt gtactgcttg tttaaattct
    50821 tgtgttccat gaggtctgta atgtctccaa catttaatct ttcttcctcc aacagttaca
    50881 ctacctgtac atgtgaattt ttctccatct ttaattactc cttcttcaag agcactggaa
    50941 gatgtaataa gcttaaatgt agaacctggt tcatatgtat cacttactgc tggatttctc
    51001 cacatttgat aatagccttt tattttatct ttatcattat atttttcaag ttcttcttga
    51061 taatatggat atattggcgt tcttgagtca ttagggtcat aatctggttt tgatgctaaa
    51121 gcaagtatat cacctgtttt agggttcata gctattgcag taaccttttt tgcattattt
    51181 aactcataag ctttctgtac tgctttttca gtatagtgtt gaattacttc atctatagac
    51241 aacacaagac cattaccttg tacaggctca taatattttt ccattccttg tggtatttct
    51301 cttcctgaag catcagtact tactataagt tttccaggct ttccttttaa ctttttatca
    51361 tattgcattt ctacaccaga tatacctgtt gcatctgaag aagtatgtcc taacacatat
    51421 ggtgcaaaat tcccatatgg gtaatatctt tgattatctt ctgccaccca tattccactt
    51481 aatttagcat ctcttatttg acttgcctta tcatcatcta tccatctttt tacttttaca
    51541 agagccatat tttttttgct tattaaagcg taaatatctt tataatcttc atccaaaatc
    51601 tcagctacct tttcggctgc ttctttttta tcttctactt ccacaggttt acaccaaaca
    51661 gtatacttag ttacacttac tgctaactct ttcatatttc tatcatatat agttcctctt
    51721 ttgggttcta ttggtatatc tcttgtttgt tgttctagtg cttttgtact caaccaattt
    51781 cccttcataa gttgtagata cccagtccta ataacaaggc aaaaaaacaa tgcacatgct
    51841 agaataagaa cgagtaccag ccttttctta cttatcctct ttacttttct caaatctatt
    51901 ccccctagtt atccacatct atatacttta tttgtccatc ttttggatag tccatattga
    51961 gcaattcctt agcctttagc tcaatttctt ttattgatgt tttttcagtt tcttttgcct
    52021 ttagttcctc taatactatc tctttttttc ttaaatcttt ttgtaaataa tagatatcgt
    52081 acttcatttc cgagattttt gaataaccac atagattcaa tattattact gaaaaagttg
    52141 caatcagtaa aatgcattta gctcctattt ttttcttatt tctcttttta taattattct
    52201 tttttttatt tttatattca tttagattat gtattttata attttttttc aaattctcac
    52261 cccgatagta tatagtttaa attttcctct atactatatt tgttattaat catatttctt
    52321 cctctaaaat ttcatcgcta aattaataca ctattacaca atctaattgt atttttttct
    52381 atacacaaca aaaacctata aattcactta tacttttata tgaatctata ggttagatat
    52441 taatttctat ttatttttat cacttctagt atttttagat aatttcttcc ataaatataa
    52501 tatgtaacta actatctact ctctgattcc taagttctcc attatattac ttaataccat
    52561 ctatatctat atttttatat ctaaattttc attatatatc tcaataacaa agaacatttc
    52621 ttatagtgtt aattgttttt taattgcttt accagtgcaa caaaatatat aatacttttt
    52681 aattaattat aataatctta ttttatatat aagattattc tttagcctta taactactta
    52741 tattttttct gctactctta gcttagcact tcttgaacgt ggatttactt ctatttcttc
    52801 ctcactaggt aatataggtt ttctagttat tattttcacc tcagattctt tatcacattg
    52861 gcatataggt aaatgttgtg gacatataca atctgatgct aaatgcttaa atgcattttt
    52921 cactattcta tcttctaatg aatggaatgt tattatacat attcttccac cttcattcat
    52981 tatagaagaa gcatcattta tcatctttgt aattactcca agttcattat ttacttctat
    53041 tcgtattgcc tgaaaagttc ttttagctgg gtgaggtcca tctattctag ctttcttagg
    53101 gatagctttt ttaattacat ctactaattc tcctgtagtt tctattggtt tgtttgctct
    53161 ttcttcaact ataaatttag ctatacgctt agcccagtta tcttctccat aatcttttat
    53221 tatcttagtt aattcatctt ctgtataagt atttacaaca tcatatgcag aaaactcaca
    53281 cctaacatcc attctcatat caagtggtgc atcttgcata taagaaaatc ctctatcagc
    53341 ttcatcaagt tggtgagatg aaactcctaa atcagctaat acaccatcaa ttttgtaaac
    53401 tcctattttt tctaattcct cttttatatt ttcaaaattg ctgtgaacaa acttcactct
    53461 actttcatat tcactcaatc tctcttttgc tgtgcttata gcatttttat cttggtcaaa
    53521 ccctataaat aatcccttat ctgacagttt ttttactatt tctttagaat gtcctgctcc
    53581 tcccatagta cagtcaacat acacgccatc tggctttata tttaaattct ctatacactc
    53641 atttaaaaga actgatacat gatgaaattc catagttacc tcccttaaat tcacttttta
    53701 aactattctt cactaatatt atttttattt ttctttgata aataaacatg tgcaattatg
    53761 ccaccaataa ctccaattag acttataact tgtgccattc taagtggtcc taacattaaa
    53821 ctatctgttc ttaatccctc tataaaaaat cttcctattg aatataaagt tatataagta
    53881 actataactt gaccttcata ttttttattt tttctaaata gtaataaaac tataaatatt
    53941 ccaaaatccc atatagactc atataaaaaa gttggatgta cctttactcc atcaaccatt
    54001 attccccaag gtaaattggt agcacctcca tgtgcttctc cattaataaa atttccccat
    54061 cttccaatag cttgtgctaa aggcattcca agtataactg tatcagccat ttttaagaag
    54121 ttaatatttt taatcttagt gtatatataa ccagctaata ttccaccaat taaccctcca
    54181 tgtatagcaa gtccaccacc tctaaaatta aaaatttgag acatattttg agcatagtaa
    54241 ctccaattaa atacaacata atagattctt gcacagatta gtccaactgg aattgctatt
    54301 atagctatat ttaatacatc atcttcttta attcccactc tttttgcttc ttttattgct
    54361 attaaagttc ctaaaatcat tccacatgcc ataagtattc cataccacat gatgtctatg
    54421 ccaaacagtg taaatgctac tctatccata acgtcacctc tttgtattaa ttgaaaatat
    54481 ttccaaagca atatgctata attacaatta attgtaatta tactaaatta tgataacaca
    54541 ttaatgttaa ttctacaaaa aatattaaaa tttattctac aactccctac actatattat
    54601 atctaattac tataaaattt tttaaactga atctctcatg actaaagtta caagtgtaca
    54661 tagatatttt ttaatcaaaa agattgatgc atatatacat caacctcttc taattcttat
    54721 ctatagctct taaattttta cctcaattta tataaatata ttttataaat ttgttttacc
    54781 aaaataaaca tttaaaaaca taaactaata cttaattagt tattcttatt atgattaagt
    54841 ttctatttag gctctacaat agaaataaca caatactctt ctttaaaatg ctcttttaat
    54901 ctttcttcaa cttcctcaaa agtaatatct ttaataacat ctaaatactc caatagattt
    54961 atatccttaa aaacataaga tataaaacta ttacctataa agtttataga atcaaagcac
    55021 ttaataaatg accctatttt tttcttttta gttctctcaa actcttcttt tgataatcct
    55081 tctttttttg atttttctat ataatcaaga attatttctt ttactttttt aggctcttta
    55141 gaatctccag ctattatgct aaaagcataa tccacttgag acgaaaatcc agctccaaaa
    55201 ttttcattta ttaaaccttg catatacaaa tcttcataaa gcttacttcc ttttttaaat
    55261 aacatcccta caagtatatc agttacaatc tctttacgta aaagttcttt cccttttaaa
    55321 ccaacattac tatctttaaa acctatgttg aacataggca tagatatagg gaatttttct
    55381 ataacctctt tttcttttac accttctggt tcttctggat aaaatctttc tatactctta
    55441 gagagtttat ctactttgta attgtttgac tttttagtta catctataac tttttctaca
    55501 tctaagtcac caacaacaaa taaagccata tttcctggat tatagaaagt attataacat
    55561 ttatatagct cttccttagt tatcttatat atactatcaa cagtacctgc tatatcaatt
    55621 ctagcaggat aatttacata cattgccttc aagcaattaa agtatacatt ccaatcaggg
    55681 tcatcattgt acatttttat ctcttgtgct attatacctt tttccttttc aacattttca
    55741 tctgtaaaat aaggtgtttg aacatagtca attagatgtt ctaaactctc ataaaaattt
    55801 tcagtagcag aaaataagta ggctgtcatt gtaaaattag taaatgcatt tgcatttact
    55861 cctaattttg aaaatttatc aaatgcatct cctccatcag gttgctcaaa catcttatgc
    55921 tctagaaaat gtgcaatacc ttcattcact ctaattttct tatcttctcc aattggtaca
    55981 aattctaagt catttgaacc ataattagta gctaaaatag catacttttt cataaaaccc
    56041 cttttaggca taaaatatac atctaatcca ttttgcaatt tctcataata aacttcttct
    56101 ttcaatatat cattaactat tttttccata gacatctcct ccattaattt ctcaagaagt
    56161 atacagtatc aagctctata ttttttatag cttcaactat atcttttttt gtaacctttt
    56221 ctatactaga tattatatct tgtacttcag aatttgtctt tgccatagat tgagagaaaa
    56281 caaaatcaga cataccacct atattatcag ttatagattt catagaatta actaaagcaa
    56341 gtttactgtt ttctaattct tcatcagata tatcacctgc ttttacactt tctacttgtt
    56401 tttttatcaa ttctacagct ttatcataat ctttagtttc tataccagat gatataaaca
    56461 tgatactttt atacttctct attgaagaga atatatagta acataaactt tctttttctc
    56521 ttacattaac aaataattta gagtgtggac caccacctaa gacattgctc ccaactacta
    56581 aagcataata tttatctatg tctgcataat ccacatttgc tctataaccc ataactaact
    56641 taccttgagt tatttccatt tgttcatcta taacttttac ctcatccaca ttttttataa
    56701 aatctgctct tggtatattt ataatttctt ctctttcaaa ttttagattt ttacttatta
    56761 tatctacaac tttatcttca tcaaaattac cttctacaac tatatctata ggtgatgttt
    56821 taagtatgtt tttataatgt tcatataact ctcttgaagt tatactatct atttcatctt
    56881 cataaccaaa ttcacttaca ctatatctct ctcctttaca catagcctca atacatttat
    56941 cttgcgcata ttccttttta tcatttatta ttgattgtat cttttctctt aaattctctt
    57001 tttcaatatt taaatattct tctttaaacc caccatctac aacaagcgta ttatttatta
    57061 cttcattaaa aaattctact acttcttcaa atatactctc atctaaatac ttttcactta
    57121 tgttaattac tttaaaactc aatacttgac gttctccacg ttttacagca tcagctccca
    57181 tagatgaacc gtataaatca tctaatttat tggatattgc tctagcacta ggatatttag
    57241 agcttccact tgctattatt gatggtatta gagcattttt tgtagtttct ttttcatcca
    57301 atagtctctg aacatataaa cttataagat tcgatttaaa cttttctgtt tttatcaaag
    57361 taagattaat attattacct aaatttatta tttttttagt attaaccata tgtacctcct
    57421 atttttattt aaacatcaat tttaaaattg aaatttattt attttttaat
//
LOCUS       DAMJWS010000014        55773 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMJWS010000014 DAMJWS010000000
VERSION     DAMJWS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.014"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            36..416
                     /locus_tag="PC399_000935"
     CDS             36..416
                     /locus_tag="PC399_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2591811.1"
                     /translation="MGFKKLPDSELKVMKFIWGLEKKVASGEVVEAMKQTYGWNKNTT
                     LTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTALN
                     DEENLSDDKLDMLEEWVKNWEEDE"
     gene            1095..2525
                     /locus_tag="PC399_000936"
     CDS             1095..2525
                     /locus_tag="PC399_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2591812.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(2673..3023)
                     /locus_tag="PC399_000937"
                     /pseudo
     CDS             complement(2673..3023)
                     /locus_tag="PC399_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3018..3539
                     /locus_tag="PC399_000938"
     CDS             3018..3539
                     /locus_tag="PC399_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2591813.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(3668..4462)
                     /locus_tag="PC399_000939"
     CDS             complement(3668..4462)
                     /locus_tag="PC399_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2591814.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(4686..8114)
                     /gene="uvrA"
                     /locus_tag="PC399_000940"
     CDS             complement(4686..8114)
                     /gene="uvrA"
                     /locus_tag="PC399_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2591815.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHQDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLKGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            8433..10667
                     /locus_tag="PC399_000941"
     CDS             8433..10667
                     /locus_tag="PC399_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2591816.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            10923..12953
                     /locus_tag="PC399_000942"
     CDS             10923..12953
                     /locus_tag="PC399_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2591817.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            12950..13402
                     /locus_tag="PC399_000943"
     CDS             12950..13402
                     /locus_tag="PC399_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2591818.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            13404..14459
                     /locus_tag="PC399_000944"
     CDS             13404..14459
                     /locus_tag="PC399_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF2591819.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            14526..14834
                     /locus_tag="PC399_000945"
     CDS             14526..14834
                     /locus_tag="PC399_000945"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF2591820.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            14938..16203
                     /locus_tag="PC399_000946"
     CDS             14938..16203
                     /locus_tag="PC399_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF2591821.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            16291..17007
                     /locus_tag="PC399_000947"
     CDS             16291..17007
                     /locus_tag="PC399_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2591822.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(17202..17495)
                     /locus_tag="PC399_000948"
     CDS             complement(17202..17495)
                     /locus_tag="PC399_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF2591823.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(17501..17716)
                     /locus_tag="PC399_000949"
     CDS             complement(17501..17716)
                     /locus_tag="PC399_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591824.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAMQQFIVENEILISEEV"
     gene            18165..18563
                     /gene="tnpA"
                     /locus_tag="PC399_000950"
     CDS             18165..18563
                     /gene="tnpA"
                     /locus_tag="PC399_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF2591825.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            18560..19723
                     /locus_tag="PC399_000951"
     CDS             18560..19723
                     /locus_tag="PC399_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2591826.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(20107..20619)
                     /locus_tag="PC399_000952"
     CDS             complement(20107..20619)
                     /locus_tag="PC399_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2591827.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            21069..21545
                     /gene="purE"
                     /locus_tag="PC399_000953"
     CDS             21069..21545
                     /gene="purE"
                     /locus_tag="PC399_000953"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF2591828.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            21551..22249
                     /locus_tag="PC399_000954"
     CDS             21551..22249
                     /locus_tag="PC399_000954"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2591829.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            22261..23628
                     /gene="purF"
                     /locus_tag="PC399_000955"
     CDS             22261..23628
                     /gene="purF"
                     /locus_tag="PC399_000955"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF2591830.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            23622..24698
                     /locus_tag="PC399_000956"
     CDS             23622..24698
                     /locus_tag="PC399_000956"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF2591831.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            24692..25285
                     /locus_tag="PC399_000957"
     CDS             24692..25285
                     /locus_tag="PC399_000957"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF2591832.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            25278..26810
                     /gene="purH"
                     /locus_tag="PC399_000958"
     CDS             25278..26810
                     /gene="purH"
                     /locus_tag="PC399_000958"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF2591833.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAVVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            26821..28071
                     /gene="purD"
                     /locus_tag="PC399_000959"
     CDS             26821..28071
                     /gene="purD"
                     /locus_tag="PC399_000959"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF2591834.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTRVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            28096..31902
                     /locus_tag="PC399_000960"
     CDS             28096..31902
                     /locus_tag="PC399_000960"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF2591835.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDNKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVIGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            32108..32980
                     /gene="rfbD"
                     /locus_tag="PC399_000961"
     CDS             32108..32980
                     /gene="rfbD"
                     /locus_tag="PC399_000961"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2591836.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            33089..33958
                     /gene="rfbA"
                     /locus_tag="PC399_000962"
     CDS             33089..33958
                     /gene="rfbA"
                     /locus_tag="PC399_000962"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2591837.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            34007..34564
                     /gene="rfbC"
                     /locus_tag="PC399_000963"
     CDS             34007..34564
                     /gene="rfbC"
                     /locus_tag="PC399_000963"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2591838.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            34602..35585
                     /gene="rfbB"
                     /locus_tag="PC399_000964"
     CDS             34602..35585
                     /gene="rfbB"
                     /locus_tag="PC399_000964"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2591839.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            35615..36283
                     /locus_tag="PC399_000965"
     CDS             35615..36283
                     /locus_tag="PC399_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2591840.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            36292..36720
                     /locus_tag="PC399_000966"
     CDS             36292..36720
                     /locus_tag="PC399_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF2591841.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            36744..37058
                     /locus_tag="PC399_000967"
     CDS             36744..37058
                     /locus_tag="PC399_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2591842.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            37210..37485
                     /gene="flgM"
                     /locus_tag="PC399_000968"
     CDS             37210..37485
                     /gene="flgM"
                     /locus_tag="PC399_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF2591843.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            37490..37906
                     /locus_tag="PC399_000969"
     CDS             37490..37906
                     /locus_tag="PC399_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF2591844.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            37924..39234
                     /gene="flgK"
                     /locus_tag="PC399_000970"
     CDS             37924..39234
                     /gene="flgK"
                     /locus_tag="PC399_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF2591845.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            39253..40185
                     /gene="flgL"
                     /locus_tag="PC399_000971"
     CDS             39253..40185
                     /gene="flgL"
                     /locus_tag="PC399_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF2591846.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            40201..40593
                     /locus_tag="PC399_000972"
     CDS             40201..40593
                     /locus_tag="PC399_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF2591847.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDSGC"
     gene            40587..40799
                     /gene="csrA"
                     /locus_tag="PC399_000973"
     CDS             40587..40799
                     /gene="csrA"
                     /locus_tag="PC399_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF2591848.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            40821..41216
                     /gene="fliS"
                     /locus_tag="PC399_000974"
     CDS             40821..41216
                     /gene="fliS"
                     /locus_tag="PC399_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2591849.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            41216..41572
                     /gene="fliS"
                     /locus_tag="PC399_000975"
     CDS             41216..41572
                     /gene="fliS"
                     /locus_tag="PC399_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2591850.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            41593..43116
                     /gene="fliD"
                     /locus_tag="PC399_000976"
     CDS             41593..43116
                     /gene="fliD"
                     /locus_tag="PC399_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF2591851.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            43119..43454
                     /gene="fliT"
                     /locus_tag="PC399_000977"
     CDS             43119..43454
                     /gene="fliT"
                     /locus_tag="PC399_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF2591852.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            43556..44428
                     /locus_tag="PC399_000978"
     CDS             43556..44428
                     /locus_tag="PC399_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF2591853.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            44521..46662
                     /locus_tag="PC399_000979"
     CDS             44521..46662
                     /locus_tag="PC399_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2591854.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            46686..48563
                     /locus_tag="PC399_000980"
     CDS             46686..48563
                     /locus_tag="PC399_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2591855.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            48556..50670
                     /locus_tag="PC399_000981"
     CDS             48556..50670
                     /locus_tag="PC399_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2591856.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            50679..51674
                     /locus_tag="PC399_000982"
     CDS             50679..51674
                     /locus_tag="PC399_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF2591857.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            51766..52998
                     /locus_tag="PC399_000983"
     CDS             51766..52998
                     /locus_tag="PC399_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2591858.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            53018..53962
                     /locus_tag="PC399_000984"
     CDS             53018..53962
                     /locus_tag="PC399_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF2591859.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            54056..54463
                     /locus_tag="PC399_000985"
     CDS             54056..54463
                     /locus_tag="PC399_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HDF2591860.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            54550..55641
                     /locus_tag="PC399_000986"
     CDS             54550..55641
                     /locus_tag="PC399_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF2591861.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        22107 a         5918 c         9802 g        17946 t
ORIGIN      
        1 atatgcagaa atttacaaaa aagggggtgc atattatggg atttaaaaaa ttacctgatt
       61 cagaattaaa agtaatgaag tttatctggg gattagaaaa aaaagtagct tcaggagaag
      121 ttgtagaagc tatgaaacaa acttatggtt ggaataaaaa tacaacacta acaatattgt
      181 ctaagcttgc aaagaaaaac tatttgtatt ctcaaaaaac aagcaagtgt acatattata
      241 ctatcgctgt aagagaaaaa gattatttaa aagttgaaac aaaaaaattc tttagttttt
      301 ttcataataa ttctttaaaa agttttttca ctgctttaaa tgatgaagaa aatttaagtg
      361 atgataaatt agatatgtta gaggaatggg tgaaaaattg ggaagaagat gaataaaatg
      421 aatgtgatac taaaatgata ctaatatgtt ttatagcgtg ttaaaaaata tagaaaatat
      481 agaaataata gaagaaaatt aatgtaatag ataaggtttt atcagattaa aaagaaaatc
      541 aatagaatgg gaataaaata tagaatgcca aaaatccaca taaatacagg atttgaggtc
      601 tctcttactt ctatgttata ctggtttgat actatcaata tttgccagta tcttatggat
      661 taataagcaa aaaacatcct gctgaatgat aaattcagcg gggtgttttt attttgtttt
      721 agaactcctt tgctctgaaa ctcgaagtat ttaacactga tgattttgtt atcttttagt
      781 tccttttgtg tcatgctctg aaagacaaat tgatacaata aaaaagtaat ttcttaatca
      841 ttgatggaga tatgtgttta tgttactctt gatggtaatt tgagtaatat tgttcatagc
      901 tttttatatt cacttaatta gaaaaccatt tggtataatc ataaataaac ttgatgatat
      961 aatagaaaag atttataatt attagaagta tataaaagta ttttatgtct atgaaatgta
     1021 tgtctataaa acatatagat gacttttgta tattaatgta tgaatattga tattatagaa
     1081 gaattgagtt gtcaatgata ctaaatcaaa tatttataga agctggaggg attatgagaa
     1141 ataaaataat tataggaatt actttttcac ttgtattact aatgtctgga tgtgcttcaa
     1201 aacaaaccgc agaaataaac gtattagcaa ctactgattt acatggagtt ataccttatg
     1261 agttaacttc ttatgtaaaa gaagaaagaa aaaaagataa aaatctaact ttagtagatg
     1321 caggagattt ttttgatagt ggagaagtgt ttggttctag catggatagt tattttagtg
     1381 ctagatataa agatggtgat aataacactg aaagttatat tgagacgcct atttcaaaag
     1441 atatgaaaga ggtaggttat gatgttgcag ttcttgggaa tcatgaattt atatccaaca
     1501 ataaatttta tctagataat atgatatctg attttgagaa gcaaaaaata aatattttat
     1561 cggcaaatac gtataaaaaa aatggagata gttatacaaa accatacata ataaaaaata
     1621 ttaatacacc tgaaggaaac gtaaaactag gaattttagg gcttactata aaagaagttg
     1681 gtgagaggaa acagtggaaa gatggaaaac ttgttgactc taagtcttta gaactaaaag
     1741 accaagaggg ttacaatggt gaattgtata tgaatgattt agtagaagat gctaaaaagt
     1801 gggttggtat aatggaaaaa gagaagcctg acattatagt tgctgtggca catacaggtg
     1861 agaaacctaa gaaacctaaa aacccaggaa atagaataca agacttagct caacaagttg
     1921 atggaattga tgctatagtt gcaggtcata atcatgttca aattgagcaa catgattata
     1981 aaaataaatc aggagaaaat gttatagtta ctgagccagg aaagcatggg gaatgtattt
     2041 ctaagattaa ttttaaatta gaaaaaaaca atgataaatg gaatatagtt gacaaatcta
     2101 gtaaattaat taaatttgaa aaaaatcaag aaagtgataa atttggagaa tttatttctt
     2161 caattaataa agtaagtaaa gataagaaag aaataagatt atcagaagta aagactcctg
     2221 aatgggataa aatatatgtt tttaaatctg gaacatctgc agatgatata tataaaacaa
     2281 taggatataa atggagaaat atatcatctc caaagggtga tgacatggtg cagattgttg
     2341 taatgaataa taataagcct gttagctatg tatatggtga agctgaagtt atgccaatta
     2401 atataaaatg tgatgaatca gagtataaag ataatgttgt aactatttat cctaataaaa
     2461 atgataaatt taaaattgaa aaaggtaaac aagaatatag tattaattta aaacatatac
     2521 aatagaaact tttatcttat ttaagtttag attgtactta aaataaagtg tgtattattt
     2581 taaaaattga tgataattaa aatcacaaca ttaaagaata gtacacactt tttatgctaa
     2641 aaaatagaat gttaaaaaat taaaccttta aattaaagta cattgcttat attatccaat
     2701 attattttca aaggttgtgt aatattttta tccttatgta atattgctaa cattttattt
     2761 tccatatcta tcccatctaa ttcaatcaat cgcaattttt tatgtaagag tgaatctaat
     2821 aataaatttt ctggcaaaat ggttatacct aaccctgctt gtgcagcttt aataagtgca
     2881 aaagaattaa cactttccca aacaggataa gctttttggt ttgaaagaga taatacactg
     2941 tccaaagtat tgcgaatggc acttccttgc tcacgtaaca ataacggaaa cgtgcataac
     3001 tgtcgtagag ataaggcttg tttagtattg gtggtggtta tgcaatcata cttttaatcc
     3061 aagagaaagt agtaaatcaa actggatgga taagtgagaa aatgtttaca gatattataa
     3121 ctatttcaca aatgacacca gggccactgg ctgttaatac ttctactttt gttggacttc
     3181 aaattggagg gttttgggga gcagttgcag ccactttagg ttgtgtatta tgtggtgtag
     3241 taatatctct atttttatat aatttttttc aaaagcatca gaactctact tatatatttg
     3301 aaatattaaa tggtctaaaa tcagcttcat taggtttaat tatctcagct gcggctacaa
     3361 ttataatcct tgcattattt gggacgaata aagtgtactt caattctata tttgagatgc
     3421 taaactggac tgcactaata atcttttttg ttacactact gatagtacgt aagtggaaaa
     3481 ttaatcctat tgtgattata ataataagtg gtatactggg aatatttttt tatttataag
     3541 tttttggtaa gtagaggata tctatttttt tatatcttaa atccaaatag ccaataaagt
     3601 aaggccattt ggatttttaa taaattctga aaagataatc agaaacccat ataacattta
     3661 aattatatca ctggttttct acaaaccaat cataagcttc ttggcaaaat ttctcactat
     3721 ctatatagta actattatga acattattaa tttcaacaag tttcttttta cattttagtg
     3781 aattataata gctatcaaaa tattgggttt taaaatatct atcatgaagt gttttaaaga
     3841 caagagtagg tatgttgact ccatctttta tcttactatc ttttgcaata aagcattcta
     3901 acattgtatt aattatcttt gtactatttt tccaaatatg aggatttttc ttttcaaata
     3961 ttttataaaa ttcattatca tagacttcat tataaccttt atatttaggt ggttttactg
     4021 ttatgtgaaa attaggaaga aacttaaaga aaaagtttaa taatttaaca gtgagatagg
     4081 tatagcctac tcctgttgtg tttttataat caacacaagc aaactgagca aaacttttta
     4141 acttattgtc tgaacatgca taatattgtg caaacatacc accaattccc gtattcccat
     4201 atagatgtat gttggaagaa taattttttt ctatgtatgt aatgacagaa tctaagtctt
     4261 ttacaattga attgcgagaa aaatcttttt catatccatc acttttccct gttcctgaaa
     4321 aatcaaaagc aaatatattt acattatgtt tggcaagtgg ttcatatgct ttttgcatct
     4381 caagcgtagt gacaatagga gtatgtgtaa caacaatatt catggatgat tttctggtaa
     4441 taaatgagtt acaaaaaatc attgtatctc catttggtat ttttatatgt ttccaactca
     4501 tttgattttc ctttctctat tataaaatat aataattttt atgttctgtt ttatatatta
     4561 ttcatatttt atcacagata atatactaaa gttaattttt ttattgtaca cctattataa
     4621 taattcttct ataatataag acattctata attttatatt taagttcatg tgtatttaat
     4681 tgtttttata taaaatgtcc agtaatacta tttggattta tttttatttc atcaggagtt
     4741 ccagaagcaa caatttcccc tccatttatt ccactgtctg gacctatatc gataatccaa
     4801 tcagaagcac gtataacctc tgtattgtgt tcaataatta aaagtgaatg ctgttcattt
     4861 acaagttttc taaaagtaat taacaaattt tctatatctt taaaatgaag tccagtagta
     4921 ggctcatcca atatgtacag cattcgtttt ttagtttttg tccctaattc ttttgctaat
     4981 tttatccttt gagattctcc tccacttaat cctaatgtat tttgtccaag ctttagatat
     5041 ccaagtccca cttcatctaa taaggagagt tttctatata tatcagagca atcgctaaaa
     5101 aatgtttttg cttctgctac ttccatgtca agtatatctg caattgtctt tcccttgtat
     5161 tgtatagaca gtatttcttt ttgatatctc ttaccttgac attcactaca agttacccat
     5221 tggtctgcca tatattgcag aataattttt gttttaccta atccttgaca gtttgggcat
     5281 tgcccctttt tgctattaaa gctaaaatag ctttcatcca ataatttttg ttttgcttgt
     5341 ggagtttcag caaatagaga acgaatttca tcaaaaatat tgatatatgt agcaggtgta
     5401 gaacggctag attttccaat aggtgtttgg tccattaaga taaaatcatc aatagcatca
     5461 aatccagtaa attcagtata atttttatct gggatagatt tttgtttcaa tttttcttct
     5521 agagcaggaa gtaaactgtg aaatacaaga gagctcttac cagaaccact aacacctgta
     5581 atagagcaca tacagtttaa gggaatttct acatcaatat tttttaagtt atttgcatgg
     5641 catcctttta agcttaacca tttacttggt ataattttat tttgtgaact tattttattg
     5701 tatgtgctta aatatttacc tgttatagag tttggatttt ttataatttc ttgggttgta
     5761 ccttctgcaa taatgaaccc cccttttgta ccagcacttg ggcctacatc aataatataa
     5821 tcagcagaca gtattgtatc tttatcgtgt tccacaacta taacagtatt acccttatct
     5881 cttagctctt ctatcatttt gatgattaaa ttatggtctc ttgggtgtaa accaatagat
     5941 ggttcatcta aaatgtaggt taagcctact aattcacttc ctagctgact tgataatcgt
     6001 actctctgca gttctcctcc agataaagtt ggagctgttc tatctgttga tatatagcta
     6061 agacctacct ttaatagata actaactcga atttttaatt ctgttaagat atcatttacc
     6121 aaagagagct catttttttg aagttgattt ggtaattcac acaaccaatt ccataactgt
     6181 tctatcgtca tttttgttag ttcagggaag cgataacctt tgattgtagt gaatctagcc
     6241 tcaatgcaca gcaattcgcc accacatgta ttgcaaggaa ttttgttcat atattgatgc
     6301 aaggtattat tttcagaact agattctcta aaaagtcttt gaatgtggtt gattgctcct
     6361 gaagcaggtc ttctaatctt tgattctcgt ccctttgatt caaagctaaa ttcatatatc
     6421 ttatcgtctg taccatataa gattgcatct ataaaatctc ttggtaattc attccaaggt
     6481 atatctaggt ctatattttt atcttttgct attgcatata attcacctac catccaatta
     6541 ccatttggct ttttcccctt caattttcca aaatagtaag tagctccatc taatattgaa
     6601 atagttgggt cttttacaat caattgagga ttaacttgat aggtaaaacc taatccatta
     6661 caatcgtgac aagctccagt atgggagtta gcattgaagg ttgaagtatt tatcttagaa
     6721 aaaatagttt gacaatgtgg acaataattt tttgtattat tctttttttc taatggaatt
     6781 ttacaatatg gacagtaact ttttccgatt gatacaaata acattcttaa ataatcccaa
     6841 atgcttgtaa gagtaccaac agtagagcga ggattttggc ttcctttttt ctgttcgata
     6901 gctatagtag gggttaatcc ctgaagtaaa tcaaattccg gtttttgaag ctgttctgaa
     6961 agttttgctc cattacttag attttcaaaa taacgtcttt ttccttcttc ataaatcgta
     7021 tcgaaagcta aagaagattt tccactacca cttacacctg taataacagt tatttgattt
     7081 tttggaattg taacctcaac atcctttaaa ttgttttgcc ttcctccttt taaatagata
     7141 gagcttttat tatgacaaag tgaattttta attgatttat ttattgattt tgtaaatttg
     7201 aatttctttt cgagttcaag aggattgcct aattctttta tttccgaaat gcctaatatt
     7261 tttaactgag aaaagattga tttcaaataa tcttcaggca atttttctat taacaaatta
     7321 aggtcttcta tttttatatt tccgcttgat aatatttctg ccatttcatc taataaattt
     7381 ttttctgctc ttgttaattg attgtatttt ttggaactta attctgtcca taaatcccat
     7441 ggaagcaatt ctaaaccaat catattatta aagtcatgta gaagattacc ctttacagaa
     7501 tgccaacctt tccaattttt attatatcca aagtcatctt gatgagcatg tccttgccga
     7561 catcttatcc aggcttctga accagttaaa aaaccatctg tttttccgac atcatgagtg
     7621 tcaaaattaa tttgaaaata atttttttgt atgtcatcta tttgtgcatc tattcttatc
     7681 catctctttg ttagagtatc atagtattct acaagccagt ggtcttcata agtaagctca
     7741 ctttcaaaat aattagcaaa acctgctctc attctagcct caaaacctcg atatcttagg
     7801 agcgatacaa gaagtaaaga ataatctctg cacattccaa caagcctttg ttttgggttt
     7861 cttggaatag ttagtgggtt agaatttatt tcaaatattc tttttaacat ttgaggaatt
     7921 gtacgaagca attcctcatc agattgttgt ttattaaagg atataccata taattttcct
     7981 tggtctccat gaattactag gctttgaacc acttttaata actcaattgg agtctttggt
     8041 aaatcttgat atttattttc aaatttttta ggatttgtga tgatgctttg atttttatag
     8101 ttatttattt tcatttatat ttcacctctc taaatggatg atataccctg acatggtgtc
     8161 atatgcaagg gcaatttaaa ttttttggcc aaaatttaaa aataaataaa aaatgataca
     8221 gtgataaatt gtaatacaaa tatagtttca aattgcatat aatataatta tttaataaaa
     8281 ccattatcta tggaatgtta atgaaatgtt aagatggcat tcttgaatat taagaaatag
     8341 aatactataa tatagtgtga aggattttta gtaaaaaaat ttgtaagtgt taatatacat
     8401 taaaaaatag atttataatt tggaggaaaa aaatgaaatt atcaaggtat aagaaaaata
     8461 gtttagttct gtctataatt ttatgttcac ttattatttc aatccttgta attactgttt
     8521 ttaataccgt aaaactacac aatatatcta ataaacaatc taaaaactat ttaaatgatg
     8581 tatctacaca gattgtaatg aatgttgatt ctaaaatcaa atttatttta tccgacttaa
     8641 gaataatggc agattttatt aagcaatatg aaggtgatag tcgatatgag tatttatcta
     8701 aaagaaagac tagttataag tattatgatg ttggaataat agatttagaa ggtaaagctg
     8761 agtttttaag tggaaaaaag tttgacttaa agaatactgt ttcctataaa aaaggaattg
     8821 aaggtaaaga atactcagaa gtaatagagt caatgggttt tgttctttat agtgtaccta
     8881 tatttaatga taatcatgaa gtatctagta ttttaatagg tgtatccaat aaagaaagta
     8941 tgaatgagat tcttaatata gataacttta atggaaaagg aactattgaa attattaata
     9001 gtaagggaaa acctcttttc ataggaaaaa atactgaatt aataaaggac cttagccata
     9061 aatataagag ttctggagat gaaccttggg cacaaaagat gcttgaggat tttaaaaatg
     9121 ataaatcagg gaatattaca ataacctcat caaaaggtgt acaatgctta ttaacttatc
     9181 atccaattac aagaggttta aatgattggc attttcttct catagtacct gaagatgctg
     9241 tacttggtga acttaataaa ttaaactctt ttacaataac aatgacattt ataattacat
     9301 gtattattgg aattataaca ttaattttat atattataag aagaaaatat gtaaatcaaa
     9361 tagaaaatat tgcatattta gatagtataa caaatggtat aaattcaact aagtttagta
     9421 tgctagtcaa acctttaatt tcagaatcac ctgatagtac ttatatgatg atagcaatga
     9481 atataaaaga ttttaagcta ataaatgatt gtttcggtag tgaaaaggga aatatgacac
     9541 taaaacatct ttacaatatt ttgtataaaa acattgatga aaatgaagag tttgtgtgcc
     9601 gtcatgatgc agaccttttc tatttgctta ttaagaatag accagtgtta gaagccctgg
     9661 agttattata taaaatagaa gctgacacaa attattttaa taaaaatagg gaaaatccat
     9721 actttttaag actatcagta ggtatttaca caattgaaaa tcataatgaa gatttaatta
     9781 caatacaaga ccatgcaaat acagcaagaa aaagctttaa taaaactcat caaagtgatt
     9841 tttcttttta tagtgatata gaaagaaaac gattaatatc tgaaaaggaa atttcaaatt
     9901 taatggaaaa agcccttgaa aataaagaat tttttatgtg cttacaacct aaaattgata
     9961 taaaaagtgg aaaaattagt ggagcagagt ctcttgtgcg ttggaaaaat tcagaaaagg
    10021 gcataattta tccaagtgat tttattcctc ttttcgaaaa aagtggattt atatgtaagt
    10081 tagatttata tatactagaa gaaacatgca aattgatttc taagtggata aaagaaggta
    10141 aagaagtgtt gagaatttct gttaatgtat cacgtcaaca tctgaatgat aaattgtttt
    10201 tagaaaaata taagcatata tgcaataaat acaatgtacc aacatgtctg atagatttag
    10261 aacttactga atctattttc ttagaaaatc cagaagctat agatataata gaggatattc
    10321 attctaatgg atttaaatgt tctatagacg attttggatt tgggtactca tcattaggga
    10381 tattaaagga ttttaaagtt gatataatta agttagaccg ttcattcttt gttagcaaaa
    10441 ataatataga ccgtggtaag gtagttatta agtctattat tgaacttagc aaaaggctag
    10501 gtatgaaagt aactgctgaa ggaattgaag aattagaaca agttgagttc ctaaaaaatg
    10561 tgggatgtga ttatattcaa ggttatgttt tttcaaaacc attattaatt tctgactttg
    10621 aaaattttgc atatgaaaat aataaaatta aaaatatcca ttattaaatg tacaagataa
    10681 aaaatttagg caagttatat aattaaaaat ataacttgcc ttttttattt atatatttaa
    10741 taaaaatata tttgcagatt ttgtaaaaat aattttcaaa tgttgtaagt tgtggctaaa
    10801 gctttattgt attattctga atatagaaag atagttactg tcttagataa aaactgaaac
    10861 atacataata atataaaatt tgctatatta ataactctaa ttattttgga aggagggaaa
    10921 atatgataag tgggagaatg ttactcatca tgaattttct aaaaggaaaa aataagacaa
    10981 gttacaagga tattagcaaa caacttagta tagaagaaag aaaagttaga tatgatataa
    11041 ataatttaaa tattgttttg cgttcattaa acaagtcagt aatacaaaag atgaacaaag
    11101 gtgtgttaat tattcctgat gacttccaag tagtatgtat aaataataac gaatatgtat
    11161 tttccttaac tgagcgagta tctattatga aaattatatc aatgtttaaa atagataatc
    11221 taaacttaga aaaattaagt aaagaatttc aggtttctag aagttcaatt aaaaatgacc
    11281 ttaatgtttt gtcaagtgaa ttagaaagaa accaaataac tattacttat gataaatctt
    11341 ttaaaataag tggagaagaa gacattatat ataagcaaag attgaacata ttaaaatcgt
    11401 attcttatct ttttggtaag gaaaaatctg atttaagtgt atttgagaag tatttagtga
    11461 atgtaattga aaaaatattc aaagggattt atttgattga tatatataat tggtctatga
    11521 atttattatt gcaaatggga tggactttaa atgatgaatc ttttacatgg tatgtgtcaa
    11581 acattttcct ttttacatgg tatatacatt cagatgaaaa acatccttta gaatctgctt
    11641 cattaagtga gccgtgtttt gaaaataagt ttgaccaaga atttgaaaca attattcaaa
    11701 aaaagttgac taaggaacaa atttttctgt taaagagttt tgtatttttt acaaataaat
    11761 atgcaagttt aaacgaggat atggatttga ttagtacaga aattatagtt caagacctaa
    11821 tttacaatat gtcagaaata ttagagatta gctttaaaga agatatgatt ctttataaag
    11881 gattactcaa tcatattgcc ccattgatag agagaatcaa gcagaatata cagatttatg
    11941 aggaattact tcatgtaata ccagataaat atcaatatgt acttgaagct actaaggttt
    12001 ctattcaaaa aaacagagtt ttaagtcaaa taaagaatga aaatgaaaca atacttttgg
    12061 caatacattt cttaggaagt atacatagaa atgagaggaa taactacaaa aatatattgt
    12121 tagtttgtgg atttggatat ggtgtaattg ctatgttaaa ggatagttta gtaaatgatt
    12181 accagataaa tatagtagag agtattccat catataagtt acaagattat aaagactggg
    12241 aaagtattga cttcattata acaacatcaa agatagtaat agacttgcct aaaacaatta
    12301 ttgagataaa tccattttta caagaagagg attatttgaa gtttgaagaa aaagggataa
    12361 aaagaaagaa tgtcttaact aattatatgt caataaaaaa aaggttggac tttttagaag
    12421 aagatgccca aaaaagagta ttaagtatta tatgtcaaga acttggatac agtgatgaga
    12481 gaatattatt tagacaactt aatttaagtg atttaatagg gattgatacc ataaggataa
    12541 ttgatggaga tatgaaatgg gaagatgctg taaaatttag ttcaaacata cttgctaatt
    12601 gtgattttgt atcagcacaa tatgctgaga atattataga tattttaaaa aatgtgggtt
    12661 tttatgctgt aaaagataac gaatttgctt tactacatgg caatgatagt tcattagtca
    12721 aagtaagttc gataagttta ttaatatgta aagaagcagt tagttttgga gataaaaagg
    12781 ttaaaatcat attttgccta gcaagcagag ataaaaaaga gcaaattcct gcaataatta
    12841 atctaactaa aatggtttat aagactaatt ttattgctat gttagaaagt gcaaagacaa
    12901 aagaagaagc agaagtttta attcataaat atgagaagga ggtcactgta tgaataaatt
    12961 agttaatgta aaatgttcat ttaaaataca tgttaattca aaagaagaag ccattatatc
    13021 gttagttgat gtagtgtcaa aagaaggata tttaatagat aaaaatcaat ttttaaaaga
    13081 tgttttaaag agagaagaaa cattgtcgac ttatattgga catggtatag gtcttcctca
    13141 tagtcaaagt gtaggtgtta aaaactcttg tattacaata ggaaagcttg atactcctat
    13201 tgaatggaca gaagaaggag aaaaagtgga tttaatattt ttgatatcag taacaaaaga
    13261 caatgaaaat aatcttcatt taaagatttt atcaaaatta gcacgtttat tgatgcatga
    13321 aagttttaga aatcaaatta gatgttcaga tgaacaaact gtttataatc ttataaaaga
    13381 aaaaatagag gaggaagatt aaaatgagaa ttttaaagtt tttgcaaaaa catcttatga
    13441 cagcaacatc ttatatgatt ccatttgtgg ttgcaggagg aatattattt gctttatcag
    13501 taactttaag tggacaagct gctgttcctg aaacaggctg gttggctaaa ctgaatcaaa
    13561 ttggagctgc tggcttggct ttattcattc ctatattagg tggatatata gcatttagta
    13621 tggcagataa accaggatta gctccaggta tgataggtgc atatttagca aaagaagtaa
    13681 atgcaggttt tataggtggt atcatagcag gatttatagc aggatttgtt gttttacaat
    13741 taaagaaaat taaattagct cctacaatga gaactttagg ttctatattt atttatcctt
    13801 tagttggtac attgattaca ggtggtataa ttgtattttt aattggagaa cctatagctt
    13861 cttttatgac atggatgaca aactggttaa atggaatgtc tggtgtttct aaaattcctt
    13921 taggtggaat cttaggagga atgatagcct cagatatggg tggccctata aataaggttg
    13981 ctgctacttt tgcccaaact caagttgaca cacttccata tttaatgggt ggagttggcg
    14041 ttgcaatttg tatacctcca attggtttag gattagctac attattgttt ccaaaaaaat
    14101 ttagcaagga agaaagagat tcaggtaaag cttcactatt aatgggatgt gttggaatta
    14161 cagaaggagc tattccattt gcaacatcag atccagttag agtaatacct tgtattatgg
    14221 ctgggtcgat tgtaggaaat attatggctt ttttattagg atgtttaaat catgcaccct
    14281 ggggaggtct gatagtgtta cctgtagttg acaataggtt ggggtatatt gcatcagtat
    14341 taactggagc agtagttgtt gcggtattaa tgaaattagt aaaaaaagac gtgaaagaag
    14401 atgaagaaat tgaagaagat ttagatgaat caattgaatt aatatttgaa gaattataaa
    14461 tataaatgga aggaatttgt ataaaaaagt aaatataaaa ctataaattt aaaggagata
    14521 agaatatgaa aattgtagca gtaacatctt gtcctagtgg agtagcacat acttacatgt
    14581 cggctgaatc acttgaatta tcagccaaaa aatttggtat agaaataaag gttgagactc
    14641 aaggaagctc tggaatagat aatgaattgt cacttaaaga tattgatgaa gcaacttgtg
    14701 taatattgac taatgatgtt gagattagaa atatggaacg ttttaaagga aagaaggtag
    14761 taagaatgag tgtatctgag ataattaaga aatctgatgc attaattaag aaaataaaag
    14821 atacttttca ataaaaattg atttatttta catataactc aaagatgttt ttaaagaaat
    14881 ttttattaaa agcatctttg aaaatatctt aaaaatagaa taaaaggaga tatagtcatg
    14941 aaaaagttac caataaagac tgtcgtgcaa agattactaa acttacaaga agaggggaaa
    15001 agtgcaactc ttttaggaat tggtcctatg tcaccaaact tgttacaagc aagttttgaa
    15061 ctagcaaaag atgatgattt tcctttaatg tttatcgcaa gtagaaatca agtagatgca
    15121 gatgaactag gtggcggtta tgtaaatggt tggaatcagg aaacttttac aaaagatatt
    15181 aagaaggttg ccgacaaagt tggatttgat ggtttatatt atttatgtag agaccatggt
    15241 gggccatggc aaagagataa agagagaaat gaccatttgc ctgttgatga agcaatggag
    15301 ttaggaaaaa aatcttattt agcggacatt gaagcaggtt ttgacctatt gatgattgac
    15361 cctacaaaag acccatttga aattggaaaa gtaattccac tagatgtagt actagaaaga
    15421 actgtagaat taattgagta ttgtgaaaac gagagaaaaa gacttaatct accagacata
    15481 ggttatgagg ttggtacaga ggaaaccaat ggaggtctga cttcaactga gacgtatgaa
    15541 acatttataa ctagactaaa ggttgaactc gataatagag gtttgccaat gccaacattt
    15601 atagttggtc aaactggtac tttaactaga aagacagaac aagtaggaac ttttaatttt
    15661 agaaatgctt atgatttagc tcaaatggct aagaaatatg gagttggttt aaaagagcat
    15721 aatggcgatt acttagatga tgtaacttta ttagaacata taccatctca aattattgct
    15781 acaaatgtag ctcctcaata tggtactgaa gaaactaggg catatttaaa attagcagaa
    15841 gttgagtgta aattagaaaa agagggctta gttgagaaaa cttctaatat cagacatgtt
    15901 cttcttgtta atgcaattga atgtggacgt tggagaaaat gggtagtagg agaacaaaaa
    15961 aatctaacta ctgaagagat atttaaggat gaagttttat ctaatgaaat actagatatt
    16021 gcaggacatt atacatttaa taatgatgat gttaaaaaag aaatagaagt gctttatgat
    16081 aatttaagta agaataatat tgatggtcaa agatttgttg tagaccatat taaacgtcct
    16141 ttgcgtgatt atgcagaatg ctataactta aaaggtgtta ctacgaggat tttaaataaa
    16201 taataggact tatagtggtg agtattgtta tactaggtat ttatagttat aattatactt
    16261 accactatta aatttaggag gactaatatt ttgattaatt taatttttga tgtagatgat
    16321 actttatata atcaattaac gcctttttac actgcatata ataaagtgtt ttcttcaata
    16381 aaggatattt ctattgaaga tttatatatg agtagtagaa agtatagtga tgaggtattt
    16441 catatgactg aaaatggaga aatgccaatc aaagaaatgc atatttatag gataatgaaa
    16501 gcttttgagg agttgggaaa ttctattaca gaaaaagatg cacaaagttt tcaagatgag
    16561 tatatatatc aacaatcaca aattacacta ataccagaag ttgagaggat tttaaatttt
    16621 tctaaagaaa gaaatattaa tctaggaatt ataactaatg gcccatcaaa ccatcaaaga
    16681 atgaagttaa agcagttaaa tattgaaaac tgggttgata aaagtaatat ttttatttct
    16741 agtgaggttg gattttctaa gccagatact aatattttta gagtagctga gaatgttatg
    16801 aatttagata gagaaaatac atattatgtg ggtgattctt atagaaatga tgtattaggt
    16861 gctaaaaaag ctggttggaa gagtatatgg ttgaaccata gaggacatga ggtagaagag
    16921 ttgttttaca aacctgattt tgtaattttg gaacataagg atttgatatc tttatttata
    16981 aaaatatgta gttataaaaa tatgtagtaa aaaaatgtta ataatagaat tgtataaaat
    17041 aattaataat aactatataa aattatatgt ttagattgtg agcaatctat tttatgttaa
    17101 aaatagatta tttagaagaa ggggaaatct taaaaaaatt tgaatatccc ctttttatta
    17161 tttaattaaa atgaaatagt tagaaatata ttttatttag tttagtcaat aacattttgg
    17221 gattctttat aactacatgt taactgttga ataggtttat catatggttt aacccatcct
    17281 ttattaatac ttaattctgt aagtgctgtg tgaccttcta ccatttgagt taatgaagct
    17341 gaatatatag ctcttaattc aggtgtacta cttgtcaatg cagaattaag atacatattg
    17401 gcagatgctt tagcagatga aagcatactt aacattatta ctttatcgtc tatataagta
    17461 ttgttcttaa ttagattttc tatcatattt ctcatgttat ctatacctcc tcagaaatta
    17521 gtatttcatt ttcaactata aattgttgca tagctttaat tcttccttct gttgctaata
    17581 tactagattc tgattgtctt tttaggtctt catctgatat taacatattt attgctcttt
    17641 gcataagtaa tccgtcagac tccattttta taactgctgc taaagatatt ttttcagttt
    17701 ctgaaagtat tctcatttaa tcatctccta tattgtaatt tatgctttaa tagtatgtct
    17761 ttcttttcaa ggaaataacc attacacttt tttacagtat tttatattct ctttatatca
    17821 aaatttagtc gacctaatat attaataata attttgtagg atatttatca ttaaatatct
    17881 ataaaataaa ttatttgaat attcataaag caaaatttat ttgtatgtac ttcttcaaga
    17941 gtcaatatga gttttttcaa aaaattaatt tttatgataa tcattaaggg ctatattttt
    18001 cttaaagttg tgattatcat tattaattta caataaaatt gccgaaaagc cctctccttt
    18061 aggtgtaggg atgaaaggta aggtcttaat ataaaaaaag ttagaataat gcttgtaatc
    18121 taataccgct actgataccc tatatttagg aggtgttagt ttttatgtat aaatctaaca
    18181 aaaatatagt ttatgattgc aaatatcata ttgtattttg ccctaagtat cgtaaacctg
    18241 ttttagttgg cgaagttgaa aagatgttaa aagaaatact accatataaa gcagatgaac
    18301 ttggagcaga aataatagaa atggaaactg ataaagaaca tgtgcactta ttaatatctt
    18361 gtgaccctca atatggaatt cataaagtag taaaaggttt aaaaggcttt agttcgagag
    18421 tattaagaga gaactttcca catttaaagt ctagtatgcc tagcatgtgg actaatagct
    18481 attttgtagg tactgttggt tctgtgagtt tagaagtagt taaacaatat atagaaaatc
    18541 aacaagttag gacaaagaaa tgataaaaac tcatagagtg aaattaaacc taactagaac
    18601 acaatttgaa ttagtaaggg aaaagcaaat ggaaagtgct aactgttgga actacatagt
    18661 taacttatct aaagaatact attttgaaca taaacaatgg attaaaaaga atgatataca
    18721 aaagttaatc aaaggtaagt acaatcttca tagtcaaact attcaagcta tatcagataa
    18781 atttgatgca aatagaaaga ctatatcaga attaagaaaa aaaggtaata ctaaagcaaa
    18841 atatccatac aagactaaga aattttatat aatacctttt aaagctagtg ctattaatag
    18901 aaatgctaaa ggcaacttaa aattatcaat gtcaaaaggc aggtatttag aattagattt
    18961 taatgttgag aatataaaaa ctgctgaaat agtatggcga aatggatatt atttatacta
    19021 cacttttgac aatgaattaa atggagttgt agccaaaggc actaataccg taggtgtaga
    19081 cttaggggaa atacattcga tagctagtgt aacaaatgaa ggtgtagggc ttattctatc
    19141 taatagagaa ggcagaagta ttaaacaatt tagaaataag atgtatgctt atatatctaa
    19201 aagattaagt aaatgtaaaa aaggtagtag acaatcaaag aagttatgga gattaaaaaa
    19261 taagataaga agtaaaacag ataatcagtt aatgaattta tatcatcaaa caactagaaa
    19321 gtttatagac ttttgtgtag agcagaaggt atgtgaaata gttcttgggg atataaaagg
    19381 ggttgaaaaa gatactaaga aaaagaaaag attaaacaga gttaatagac agaaaatatc
    19441 tcaaatggag tatggtcgaa taaaggacta tataaaatat aaagcaaaag agcaaggtat
    19501 tgaagttaag ttagttaaag aaaactacac ttctcaaact tgcccaaagt gttctaaaaa
    19561 acacaaacca actggtagaa cttatagttg tacttgtggt tatgaaactc atagagatat
    19621 agttggtgct tggaatattt taaataaaaa gcataaatat ggtttagtag attttagaat
    19681 aaatcataag caaccaataa atttaaaagt atctaccgtt tgagccatgt gtgggcgaat
    19741 aactttaagg tagtagtggc gaagacaccg tccgaagtag cttggctttg taaccacctc
    19801 tgttgtactg tttaagtata aataagtgtg ttagccacca agaatcccct tgctttagct
    19861 atggggagtg tgtcaataaa atactttaat gaaaaatagc taactttaat tgtaggatat
    19921 tcttaatata tttgaaataa ataaaaatat tgaaggatat aatattcaat tggtatataa
    19981 ataataaatg atagttaaat tttaccttag aggctaatta ggtgtttagt caaagctaat
    20041 gcctttagaa tacaaatgta ttctaaaggc atttttacta tgtattttca tttatattac
    20101 taaacattat ctatttttat atacatttct atgtatttta ttttcatcaa atcggtctat
    20161 aaactcattt ttttcttcac tatatccaat agatataagt gcataaggaa ctaattttaa
    20221 tggtaagtct aaagtttgtc taactttatt taccacttta tttttaggaa atactcctgc
    20281 ccaacaagca gctagacctt gattagtaac ttctaataat aaattttgtg tgcaagcacc
    20341 taaatcttgt atccatttac caggttttaa gaatttatct ctatttccaa gcactactat
    20401 acataatggt gcaaattcta tatgctttgc tctatgctgt gactttgaga gctgtttcag
    20461 cagttcctta tcatctacta taataaattc ccaaggttga gcattttttg atgaaggtgc
    20521 ttgcatagct gattttagta tttcagttat tttatcttct tctatatttt tatctatata
    20581 tgttcttatt gaacgtctat tttttataac ttctaacata cagattctcc tttaaataaa
    20641 attatttcta tttatataat atcattactt taaattttag ctattactta tataaagaat
    20701 aaactttgtg ttggttttat ctaagtaaaa gtaacattca gaactaaaaa acaagactat
    20761 aaatccaaag tattaattaa aaaagaagta ataaaaaata atatacaaga tttaaaaaag
    20821 agtattgggc ttaaaattta attagaaaac caattttgag ataatctgtt ttttatttta
    20881 acaaaaaaac acaaataaag ttcgatacgt agcgaatgaa aaaattacaa gaaatatttt
    20941 ttttaaaaaa tttcaaaaaa gagttgataa tatatgtata ttaatataaa atttaaggtg
    21001 tataattcgt ataataatac gaatgatttg tagtgtaaaa taacttataa acggagaggg
    21061 gaagtctaat gaaagtagca gtagttatgg gttcaaaatc agattatcca aaattagaag
    21121 aagggataaa actgcttgaa aaatatggaa tagaagttgt agcaagagca ttatcagctc
    21181 atagaacacc agaacaactt tcaatattcc taaaagaaat agaagatgat actgatgtaa
    21241 taatagcagc agcaggaaaa gcagcacact taccaggtgt aatagcttca caaacattaa
    21301 ttcctgtaat aggattacct attaaatcat caactatgga tggactagac tcacttttat
    21361 caatagttca aatgccaaaa ggaatcccag ttgcaacagt tacaatagac ttaggtttaa
    21421 acgcagcttt attggcatta caaataatga ctttaaaata cccaaaatta aaagaggatt
    21481 tgaaatcata tagagaagag atggcacaaa aggtgttaga agacgacaag aatttaaggg
    21541 ggtagcagaa atgttattat atgaaggaaa agcaaaacaa gtatattcta cagataatga
    21601 aaatgaatat gttgtttatt ataaagatga tgcaacagca tttaatggag aaaaaaaagc
    21661 agaaatatct tcaaaaggta tactaaataa taagatatca acaataatct ttgaaatgtt
    21721 aaaagaaaat aatataaata ctcactttat aaaaagttta tcagatagag aaatgttagt
    21781 taaaaaagta gagattttac cattagaagt aatagtaaga aatattgctg ctggttctat
    21841 ctgtaaaaga gttggtcttg aagaaggtgt agttttcgat gagccaattt ttgaaataag
    21901 ctacaaaaat gatgcttatg gtgacccaat gctaaatgat gattatgcag tggcaatgaa
    21961 gttagcaact agagaagagt taaaattctt aagagaagaa actttaaaaa taaatgagtt
    22021 actaaaagca tttttcttaa aattaaacct aaagttagtt gactttaaaa tagaatttgg
    22081 taaggattct gaaggaaata taatattggc agatgaagta tcaccagaca cttgtagact
    22141 ttgggatgta aatacaaatg aaaagttaga taaggacaga tttagaaaag accttggaga
    22201 ccttgtagaa ggttatacgg aagtactttc aagaatgaat aataaatagg aggcaaatgt
    22261 atgtgtggag ttttagggat ttactctaat aaagatgtaa ctaaagaact atattattct
    22321 ttatattcta tgcaacacag ggggcaagaa agctgtggtt tagctctttt agatgatgga
    22381 gaaattaagt acaaaaaaga tatgggatta gttggagatg tttttaagga aaatgaatta
    22441 tctaagttaa aaggaaatat agggattgga catgttagat attcaacagc tggaggaagt
    22501 catgtttcta actgtcaacc tttagttgga agttgtagaa aaagacaatt agccatagct
    22561 cataatggaa atctagttaa tgcaaattat ttaaaagata tgctagaaga agatggatat
    22621 atgttccaaa ctaattcaga tacagaagtg attttatata tacttgctag atattacaaa
    22681 ggggatatag tagaaagttt aaaagtgaca atggattata taaaaggtgc atatgctctt
    22741 gtaataatga gtcaagaaga attggttgca gttagagacc cacatggatt cagacctctt
    22801 gtacttggta aaaagggtga tgaatatata tttgcatcag aaaattgtgc aatagatata
    22861 cttggcggag aagttataag agatgtagag ccaggagaga taatagtagt caaagatgga
    22921 gaactaaaat catattttta ttcagaaaat tataaaccag ttaagaaaag ttgtatattt
    22981 gaacacatat actttgcaag aaatgatgca actatagaca atgtaaatgc atatgaattt
    23041 agaattaaat gtggtgagag attagctcag aatgaaactg taaaggcaga tatggtagtt
    23101 ccagttccag attcaggttg gccaggagct attggttatg caaatgctag tggattaaaa
    23161 ataagtgaag gcttagttaa aaatagatat gtaggaagaa catttataaa acctacacaa
    23221 gaagaaagag aaatagcagt aaaaataaaa ttaaatcctc tttcaacaat aataaaagga
    23281 aaatctataa ttttagtaga cgactcaatt gtaagaggaa ctacatctaa acaattagta
    23341 aaatctttaa gagaagcagg agcaaaagaa attcatctta gaataacttc tcctccagta
    23401 gcttactctt gttactatgg aatagatact ccaaatcgct ctaaattaat tgcatcaagt
    23461 aacaacgtag aagaaatgag agaatatatt ggatgtgata gtttaaaatt cctagatata
    23521 gaaggaatgt tagatgcaac agaacataag tctactttct gtaaagcttg ttttgatgga
    23581 gaatacccag ttaagaaaat tgataaggag gaactacttt catgttaaca tataaagaat
    23641 caggtgtaga tatagatgaa ggaaatagag cagtagacct tataaaaggt aaaataaaag
    23701 gaacttatga tggaaatgta gttggagatt taggtaactt cagtggatta tacagtttaa
    23761 aagattttgt aggtatgaaa gaaccagttt tgcttgcatc tacagatggt gttgggacta
    23821 agttaaaaat agctcagatg atggacaagc atgatactgt tggaatagat ttagttgcaa
    23881 tgtgcgtgaa tgatttaatt tgtcaaggag caaaaccatt attcttcctt gactatatag
    23941 ctcttgggaa attagtgcct gagcatatcg aaaaaatagt tggtggaatt gcagatggtt
    24001 gtaaaatgtc tggttgtgca ctaattggtg gagaaactgc tgaaatgcct ggaatgtatg
    24061 gagaagatga ctatgattta gcaggatttt ctgtaggaat agcagacaaa gaaaagattg
    24121 tttctggaaa caatgttaaa tcaggagatg tattggttgg tatatcttca agtggagtac
    24181 acagcaatgg attttctttt ataagaaaga tatttttaga aacttacaat tataaaatgg
    24241 aacaatatgt tgaagaacta ggaatgacag ttggagaagc acttttaaca ccaactaaaa
    24301 tatatgttaa attagcttta gatgtattag ctaaacatga cataaaagct atagctcaca
    24361 taactggtgg agggcttata gaaaatataa caagagttat acctaaaggg ttaggactag
    24421 atatcaataa aaaatcttgg gagaagcctc ctatatttaa aatgatagaa ggttttaatg
    24481 ctgttgatga gagagaactt cataagagtt ttaacatggg tattgggcta gttttaatag
    24541 tagataaaga aaatgctgat gatgttgtaa actttataaa taatagagaa aatgacaatg
    24601 ctgcttatgt agataaaaaa tatagtgaat tattagaaga taaagcttat ataattggtg
    24661 aagtagtaga tagtcatgaa ggtgtagaat tatgctaaat ataggagttt taatatctgg
    24721 tggaggaaca aatttacaag cagttataga tggtactgag tctggagaaa taaaaggtca
    24781 ggttaaagta gttatctcaa gtaagcaagg tgcttatggg ctagagagag ctaaaaatca
    24841 taacataaaa gcaatttgtg aaactgatga agacaagata atagaaattc ttaaggaaaa
    24901 taaaatagat ttagtagttt tggctggtta tttaaaaata atcagcccaa agctggttaa
    24961 cgagtttaga aacaaaatga taaacataca tccatcatta attccttcat tttgtggagc
    25021 tggattctat ggagaaaaag ttcatcaagg tgttatagat tatggagcta aagtaactgg
    25081 tgctacagtt cattttgtag atgaaggtgc tgatactgga ccaatcataa tgcaagatgt
    25141 agttaaggta aatcaagatg atgatgcaaa gacacttgca aaaagagtgt tagaagttga
    25201 acataggatt ttaaaagaaa gtataagtct tttttgtgaa aataaattaa aacttcaagg
    25261 taggagagtg tttataaatg agtaagagag ctttaataag tgtaactgac aagactggtg
    25321 tagtggaatt tgctaaagag ttaaataaat tagattatga aattatatct acaggaaata
    25381 catttaaaac attaaaagaa aatggtgtta atgtaatgca agttgaagat gttacaaatt
    25441 ttccagaaat attagatgga agagtaaaaa cattaaatcc atatatacat ggtggtatac
    25501 tttataaaag agataaagaa tctcatgtag aaactgtaaa tgaacacaaa atacattcaa
    25561 tagatttagt agctgtaaat ctatatgatt ttgaaggaac tttaaaagct ggaaaaagtc
    25621 atgatgaaat aattgaaaat atagatattg gtggaccatc tatgatacgt tctgctgcca
    25681 aaaactataa ggatgtaata gtagtagtag atataaaaga ttatgattct ataatagaaa
    25741 aattaaaaac agatacaatg acattagaag acagaaaaaa attatcatat aaagctttct
    25801 caactacagg aagatatgat gcgttaatat ctagttattt tgctggagaa gttggagata
    25861 cgtatcctga catattaaat ttaactttcc aaaaagaaca aactttaaga tatggagaaa
    25921 atccacatca aaatggtttc ttatattcac agtcaaatgc aaaaaatcca atcttaaatt
    25981 atgagcaatt aggcggtaaa gaactttctt ttaacaactt aaatgattta catggatgtt
    26041 tagaagtaat gagagaattt aaagatagtg aagaagtagt ttcagtagct ataaaacatg
    26101 ctaattcatg tggggtaggt ttaggaaaag atgcttttga agcatataca aaatgttatg
    26161 aagcggataa ggtttcaata tttggaggaa tagtaggaat aacttctact atagataaag
    26221 ctacagcaga gaaattaaat gaaatattct tagaaatagt agtagcatat gattttgaac
    26281 cagaagcttt agagatactt aaacaaaaga aaaatctaag aatattaaaa ttagctaaaa
    26341 tagaaaatag tttacaacca tatgaaatga aatatttaga tggaaaatta ttgatacaag
    26401 atagaaacaa tatattagca gaaaaaagtg aaaatgtaac aaaggaaaaa ccaacagatg
    26461 cacaacttaa ggacatggaa tttggtatga gagtagtaaa aaatatgaaa tcaaatgcaa
    26521 ttgcagtagt aaaaaatgga caaactctag ctttaggttg tggacaaaca tcaagaattt
    26581 gggcattaaa aaatgctctt gaaaataaca aagataaaga ttttacagga gctgttttgg
    26641 cgtcagacgc atttttccca tttgatgatt gtgtaacatt agctcatgaa tatggaatat
    26701 cagcagtagt tcaaccaggt ggctctataa aagataaaga ttcaatagaa gcttgtgaca
    26761 agtatgatat ggttatggta tttacaggaa taagacactt caaacactaa ggaggatatt
    26821 atgaagatat tggttgtagg cggtggcgga agagagcatg ctatatgctg gaaactaagc
    26881 aaagaaaaaa atgtagaaaa aatttattgt gcaccaggta atgcaggaat tgcaaacgta
    26941 gcagaatgtg taaatatagg tgatactaat atagaagaac ttttaaaatt tgccaaagaa
    27001 aatgaaatag gtctgacaat agtaggacca gaagtaccac tagttatggg aatagtagat
    27061 gagtttgaaa aagaaggatt aagagtattt ggacctaata aaaaatgtgc tcaacttgaa
    27121 ggaagtaaag ctttttcaaa agaatttatg ataaaacata acatacctac tgcaaaatat
    27181 aaagaatata caaacttaga agaagctata tcagaaatag atagttttgg atatccagta
    27241 gtaataaaag cggacggatt agctgctgga aaaggtgttg taatacctga aaatagagaa
    27301 gatgctatag caactttaaa agaaatgatg tcagataaga agtttggagc tgctggagat
    27361 aaaatagtaa ttgaagagtt tttaaaagga atagaaactt caattctagc atttgtagac
    27421 aatgatacaa tagttcctat ggcaagtgca aaagaccata aaaaagtaaa caactatgag
    27481 caaggtccaa acactggagg aatgggaact ttttcaccta gtgaaatata tacagaagaa
    27541 ttagcaaata aagtaaaaga gacagtatta gaaaaaactt tagaaggttt caaaaaagat
    27601 ggacttaatt ttaaaggtat tttatttgtt ggtcttatga taactgaaga tggagaaaaa
    27661 gtacttgaat ataatgtaag atttggagac cctgagacac aatctgtttt atttagatta
    27721 gaaacagatt tacatgagat aatggaagct atactagata ataagttaaa agatatagaa
    27781 ataaactata gtgatgatga agctgtatgt gttatgctta catcaggagg gtatccagac
    27841 agttatgaaa aagggaaaat tataactggt cttgaaaact tggatgatga tatagtagta
    27901 ttccatagtg gcactaagat gtttgatggt aatttggtta ccaatggagg aagagtaatt
    27961 ggtataactg caaagtcaac tagagttaag gatgcagcag aaaaagttta tgaaaacata
    28021 aagaagataa actttgaagg aatgcactat agaacagaca taggaagata atttttctaa
    28081 aatggaggat aaggtatgtt gaatactgaa aataaagact caatggttag aagagtattg
    28141 gttgaaaaaa gagaaggttt tgaccttgag gctaaagctt taaaaaaaga cttagtagaa
    28201 agtttacaca tagataatat agaaaattta agaatattaa atagatatga tgtagaagga
    28261 atatcagaag aagtttatga aaatgcagca aagacaatat tctctgaacc aaatttagat
    28321 gtagtatatt atgaagaaat accaaaatta aatgatgaaa gagtatttgc aatagaattt
    28381 cttcctggtc aatacgacca aagaggtgat tgggcagctc aatgtgtaca aattgtaaat
    28441 caagggataa gacctgctat taatacagca aaagtttata tattatcagg caaaataact
    28501 gatgaagagt tttctaaaat aaaagattac tgtataaatc cagtagatag tagagaagca
    28561 tctttagaga aaccagaaac gttaaaaatg gaaactgaaa taccaacaac agttgaagta
    28621 ttagatggat ttatagattt agatgaaaat ggattaagaa catttgtaag tgaaaaagga
    28681 ttagcaatga ctttaggaga cttacaacac gttcaaaaat actttaaaga tacagaaaag
    28741 agaaatccaa ctataacaga aataaaagta ttagatactt attggtctga tcattgtaga
    28801 catactactt ttatgactga aatagaaaat gtaaagatag aagatggtaa gtttaatgat
    28861 atagtaaaag aagcgtatca aatgtatctt aactcaagag acaatgtata tgtgaacaga
    28921 cataaagata tatgtttaat ggatatagcc actgttgcag taaaagaatt aaagaaaaat
    28981 gggaaattaa atgacctaga tgaaagtgaa gaaataaatg catgtagtat aaacgtagat
    29041 gttgaagttg atggtaagat ggaaaaatat ctagtaatgt ttaaaaatga aactcacaac
    29101 catccaactg aaatagaacc atttggtgga gcagctactt gtttaggagg agctataaga
    29161 gacccattat caggaagaag ttatgtttat caagcaatgc gtgttacagg aagtgctgac
    29221 ccaagaacga ctttagaaga tactttacct ggaaaactta tgcaaagaaa aataactaca
    29281 gaagctgctc atggatatag ttcatatggt aaccaaatag gtcttacaac tggacaagtt
    29341 gcagaagtat atgatgaaaa ctttgttgca aagagaatgg aaataggtgc agttatagca
    29401 gcagcaccaa aagagaatgt agtaagagaa agacctgaag caggagatgt aatcgttctt
    29461 cttggaggaa aaacaggaag agatggctgt ggaggagcaa ctggttcatc taaagaacat
    29521 agtgaagagt caatcttaac ttgtagtgca gaagttcaaa aaggagatgc tcctaacgaa
    29581 agaaaaatac aaagattctt tagaaataaa gaagttgctc aaatgataaa aagatgtaat
    29641 gattttggag caggtggagt ttgtgttgct ataggagaga ttgctgatag cttagatata
    29701 aacttagact tagttcctaa aaaatatgat ggacttgatg gaactgagct tgcaatttca
    29761 gagtcacaag agcgtatggc agtagccata aaaaaagaaa ataaagataa atttatacaa
    29821 ttagcagtag aagaaaactt agaagctacg catgtagcta cagtaacaga tacaggatat
    29881 ttaagaatgt tctggaatgg aaaagcaatt gtagatataa atagagagtt tttagataca
    29941 aacggagtaa aacaaactac tgatgttcat gttactaaag tagatgaaga aaatacattc
    30001 tttagctcta atgaaatagt aaaagatgtt aagtgtgcaa gtatgaaaga caagtttaca
    30061 aaagtactat ctgaccttaa tgtttgttct caaaaaggat tagtagagat gtttgataat
    30121 acaattggtg gtaatacagt acttatgcca tttggaggaa agtatcaagc gactccaaca
    30181 caaggtatgg ttgctaagat accagtactt ggaggagaaa ctaatacttc tacaataatg
    30241 acttatggat acaatccaaa ggtaggaaaa tggagcccat tccatggtgc cttgtatgca
    30301 gtagtagaat ctgtgtgtaa attggttgct attggaggaa attatagtac aacaagactt
    30361 acattccaag agtattttga aaaacttggt aacaatccag aaaaatgggg taaaccattc
    30421 tcagcacttt taggagcgtt ttatgcacaa agtaaatttg agatacctgc tataggtgga
    30481 aaagacagta tgtctggtac atttaaagat attgaagttc caccaacatt agtttcattt
    30541 gctgttgaca cagtagatgc gaagaaagta gtatctccag aatttaaaaa agcagactca
    30601 aaagtagtaa tgttatgtgt aaacaaggca gaaaatgatg ttgtagattt tgaggaatta
    30661 aagagaaact tagataaagt aagagaactt atacatggaa ataaagtttt atcaacatat
    30721 gcattaggat ttgcaggtgt aggtgaagca ataagtaaaa tggcttttgg taataaaata
    30781 ggatttaagt ttagtgaaga agctgaaaaa gcatttacag ataataaatt atttgaggct
    30841 agttatggaa atatagtact tgaattagca aatgatgatt taagtatgtt agaaggatac
    30901 aattatgttg tgattggaag tacagttaaa gaagctagta tatttatcaa aggtgaagaa
    30961 cttgcattag atgaattata taaagcacat tgtagtactt tagagcctat attcccaact
    31021 aaaacagaag aagtaaaatc aaagatagaa actataagtt atatatcaca aggagaagct
    31081 aaaaaatcat cattaagtat agctactcca agagtattta taccagcatt ccctggaaca
    31141 aactgtgaat atgactctgc aagagcattt gaaagagcag gagctaatgc aagtataaga
    31201 gtatttaaga atttaactta taaagacata gaagattcta tagatacaat agtaaacgaa
    31261 ataaaatctt ctcaaataat aatgttacca ggtggattta gtgctggtga tgagccagat
    31321 ggttctggta aatttatagc tactgtattt agaaatccta gagtgcaaga agctataaat
    31381 gaatttttaa ctcaaaaaga tggactaatg ttaggtatct gtaatggatt ccaagtatta
    31441 attaaattag ggttagttcc atatggagaa ataagagttc caagtgaaag tgctccaaca
    31501 cttacttaca ataatattgg tcgccatcaa gctaagattg ctagaactag aatttcatct
    31561 aataaatctc cttggttagc tcaaactaat gttggagata ttcataatat agcaatttct
    31621 catggagaag gtaagtttgt tgccagtgaa gatgttatga gagaattaat tgctaatgga
    31681 caagtagcaa ctcaatatgt tgactttaat aatgaagcta cttatgatat tgagtttaat
    31741 ccaaatggtt cattctatgc tgttgaaggt ataactagtg ctgatggtag agtgtttggt
    31801 aaaatgggac attctgaaag aattggtgag gaagtatata agaatattat aggtgaaaaa
    31861 gaccaaaaga tatttgaatc tggagttaaa tattttagat aaagctttga tgtttaattg
    31921 atagctagaa tatataattt agatatttag atttaaagag atgtaataat atgatgataa
    31981 tcatttaata ttacatctct tttttattta tctaagaaat aataagttca acctaagtta
    32041 aaatacaagc tgtgttataa ttaattatta agtaaaattg aatatttatt ggagggcaac
    32101 ataattaatg aaaatattaa taacagggtc taatggtcaa ttaggaaaag aattggttaa
    32161 ccaattagaa gcaatcaatc aaagtataaa ccaaccaaaa tatgttatat tagctacaac
    32221 aagaagtgat ttggacatat ccaatcagac taatgtagat aattttattt tgcataataa
    32281 acctgatgta gttgtaaact gtgcagcata tacaaaggtt gatgcatgtg aagataatat
    32341 cgaaattgca tacaaaataa atgctttagg agtgagaaat ctagcaatag catcagaaaa
    32401 agttaatgct aagcttattc acatatcaac tgactatgta ttcaatggtt tttctaaata
    32461 tccatatagg gaagacaata aaactgaacc caattctgta tatggaaaaa gtaaacttat
    32521 gggagaaaaa tttgttgaac aattctcaca taagtacttt atattgagaa cagcatggtt
    32581 atatggtaat gggaataatt ttgtaaagac catgataaaa ctttctttag aaaataaaga
    32641 ggttaatgtg gttaatgacc agtttggaac tccaacaagt acagtagact tagcaaaaat
    32701 tataataaga atcatggaaa cagaacatta tggagtttat catgctacat gtgaaggtga
    32761 gtgtagttgg tatgattttg ccaaaaagat atttaaacta aaaaatatag atataaaagt
    32821 aaatccaatt aagagtaacg aatttaaatg taaggctcca agaccactat attctgtatt
    32881 ggataatttt atgttgaagt taattggttt aaattctttt agaaagtggg aagaatccat
    32941 tgaagaatat ttagaaagat atgatataaa tgaaaagtaa atgtattatt ttgagaaaag
    33001 aaaataagat attataaaaa atataaatat ttcttttatt tatccgataa tcataatatg
    33061 acaaaaacaa atttaaggat ggaaaaagat gaaaggaatt attttagcag gagggtctgg
    33121 tacaagacta tatcctataa caaaatgtat atcaaaacaa acacttccaa tatacgataa
    33181 accaatgata tattacccta tgtcggttct tatgttggca gggatacaag atatattaat
    33241 aatatctaca ccaagagata taaaagcatt tgaagaatta tttggagatg gaagttcact
    33301 agggcttaac ataacatatg caattcagga gaaagcaaat ggaattgcag aagcgtttat
    33361 aataggagaa gaatttatag gaaatgacag agtgtctctt gtattagggg ataatatatt
    33421 ttatggatat ggatttagtg aaaggttgga gaatgcagtt aaaagaaaag gagcaactat
    33481 atttgggtat catgtaagta atccaaattc gtttggagtt gtagaatttg ataaggattt
    33541 taatgtattg tcaatagaag aaaaacctac agttcctaaa tctaattatg ctataccagg
    33601 attatatttt tatgataatg atgttgtaga gatagcaaag ctagttgaac cgtcagatag
    33661 aggtgaatta gaaataacct ctataaacag tgaatatcta aaacgaggaa aactaaaagt
    33721 ggaacttcta ggaagaggta tggcttggct tgatactggt acacataaag gtctattgga
    33781 tgcttctaat tatgttgaag tggttcaaac aagacaggga ctgtatatag catgtttaga
    33841 agagattgca tatacaaaag gatatataag tagagaacaa ttaatgaaac tagccaagcc
    33901 actgataaaa actgaatatg gtcaatatct attaaaattg gctagtgaag atatgtaata
    33961 tggtagataa aatttattat aaattttatt aagggagttt attataatgg gaaaaatcaa
    34021 tgttataaag actaaaatag aaggattata tatagtcgaa ccaaatgtat tttatgatga
    34081 aagaggatat tttatggaaa gctataataa aaaggatttt atagagcagg gtcttgatat
    34141 gtgttttatt caagataatg agtctagctc aagaaaaggc gttttaagag gaatgcattt
    34201 tcaggttaaa tatcctcaag gaaaattagt cagagttaca gagggagaag ttttcgatgt
    34261 tgcagttgat ttaaggaaag attcacaaac atttgggaaa tgggaaggtg taatattgtc
    34321 tgataagaat aaaaaacaat tttatattcc agaagggttt gcacatggat ttttagtttt
    34381 atcagaaaaa gcagtattta gttacaaatg tacaaattat tattacccag aatttgagaa
    34441 tggctttata tggaatgaca agcaaatatc tattgagtgg cctttaaaag gaattgaaga
    34501 aatattttta tcagaaaagg acaaaaaaca aaaaagtttt aaagatattt gtacaagacg
    34561 ttaataaaaa tttattagaa ttaacattta ggagaaaaaa tatgaaaaaa atactgataa
    34621 ctggaggagc aggatttatt gggagtaatt ttatacatta tatgcttaac aagtatgtag
    34681 attattttat tgttaaccta gacttgttga cttatgctgg taatttagag acattaatag
    34741 aaatacaaga caattccaat tataaatttg ttaaaggaga tatttcaaat agggagcagg
    34801 tgtataagct atttgaagaa gaaaggtttg atatagttgt taactttgcc gctgaatctc
    34861 atgtagatag gtcagttgag aatcctgatt tatttattaa aacaaacatt ataggaacac
    34921 aagtactttt agatgcatct ataaaatatg gagttaaaag gtatcatcaa atatctacag
    34981 acgaagtgta tggtgattta ccaattgata gaaaggattt attctttaca gaacaatctt
    35041 ctatcaatcc aagttctcca tattcagctt ctaaagcatc agcagattta ttggtaagtt
    35101 cttattatag aacatatgga ttgttaacaa ctatatctag gtgttccaac aattatgggc
    35161 catatcattt tcctgaaaaa ttgattcctc ttatgatttt aaatgctttg gaaaacaaac
    35221 aattgccagt atatgggaat ggggaaaatg taagagattg gttacatgta tatgaccatt
    35281 gtactgctat agacttaata atacataaag gaaatatagg ggagatttac aatataggag
    35341 gtcataatga aagaagtaat ctagaagtag taaaaatgat attaaatctt ttaggtaaat
    35401 ctgaagagtt aatatcctat gtaaatgata gacctggtca tgatttaaga tatgctattg
    35461 atgcaactaa aatagagaat gagttaggtt ggaaggcaaa atatgacttt gatttgggta
    35521 taaaagaaac tgttaagtgg tatatagaaa atgaatcttg gtggaaagct gttttatcag
    35581 tataacttta aaatttagaa aggggcgttt tatattgaat gagataagtt cactaattaa
    35641 tatattagca ttgactcaat tatctggtag taatactaca aatcaatgta attgtacata
    35701 taataattca tctggatttg atatgattat gatgaccttg ttaaaagcat tatcacaaaa
    35761 taatcaacat acttctaata gctacaattt attgtctaat tctgagaatt tatttaatga
    35821 acttgataat gtagtaaata ataccactag tagatttata gatgttgata caaaagataa
    35881 aaatgtaaag ttgagaattg aaaatgctgt agatcaagca tcaaaaaaat ataacgtaga
    35941 ttcaaatctt ataaaagcaa taattaaagt tgaatcagat tttaatccaa ataccgtttc
    36001 ttctgctggt gctaagggtc ttatgcaact tatgccagaa aactgcagag acttaggagt
    36061 tacagaccct tttaatatag aacagaatat agatgctgga actagacata ttaaggaata
    36121 cattgatatg tttggaggga gcatagaaat gggattgatg gcatataatg gtggtcctgg
    36181 aagaatgaaa agtagaggag tagaatcaat ctctgacctg tataaaatgc caaaagaaac
    36241 acaaaattat attcctaagg ttatgaaata ttatagaggg taggctttat tatgggaata
    36301 aatatagata agctaaacat aagtccaagt ataaatatgg aggttaaaaa actaaaagag
    36361 agtaataaat tttctgacaa attaaactct ttaaaacaat cttcttcatt ggaagatata
    36421 aaacgaaatt tggaaagtgt aaaaaaggca ggagaaaggc ttgtacttac tcaaaactat
    36481 ggcgatatta gtatatataa aaatgcagta aaagaatacc taaaatctgt tgtagataat
    36541 atgtattcac ttgataaaaa tagtagcttt tgggagcatc agtattataa gaatgtaaag
    36601 attgttgata gtaaacttga ggatatgact agtaagttaa ttagtgaaga aaaagaaaac
    36661 atagatattg tttctactgt tgacatgata caaggcttat tgatagatat gtatagataa
    36721 ggttatacaa ggagaaaaat tttatgaata gcaaaaaaga ggatatatat gaattgaatt
    36781 ttgggcaatt aatagaagaa aaatctggtg ttatagaaga aaatcaaaat gtttttaatg
    36841 caaaagtaaa tgtagctgtt tctataggaa atacaacaga aacaataaaa aatatattag
    36901 atttaaaaga aggctctata ataaaattaa gtaagaatgt ggatgaaaaa ttagatatat
    36961 atgcaaacga tagattgttt gcttatggag aaagcattat tacaaatgag aggttatctg
    37021 taagattatc taaaatagaa gatccagatg taaattagca ttttaaatag tatcccttta
    37081 ggctattctg tatgagttta gctaagggga tatttttata aaaaatttaa aatatgtgtg
    37141 aattatataa atatttttat tttatgagcg ataactaaat tataagtatg taattttgga
    37201 ggtgaaacta tggatattaa aaatgtaagt tcaaatattg tagtgaataa ttatttaaag
    37261 aatacatctg gtataaaaaa agtggaagag gttaaaaaaa ttaataaaac agataagata
    37321 gagttatcta aatttgcaaa ggaaatggca agtgtgtcaa atatgagtga tgagaatatt
    37381 agaaataaaa aagtcgatga aataaaaaat gcattagaaa atggaactta caaaataaaa
    37441 ccaaaagaaa ttgcgaaaaa aatgatagag gatatgcgag gataaagtca tgattacaga
    37501 cttaaaagtt ataatatatg aagaaaaaaa tataattagt aagatgatgg aattattaaa
    37561 agaacaattt gattatatag ttgaaaaaga cttagaaaat ttaaatatgt taaatcctaa
    37621 actagaagat attagcaggg agcttgcatc catagaaata aagagaagac aactttttgg
    37681 tgatgatatt tcaatgagtg aagttgttga aaatagtaat gatggatact taaaagaaat
    37741 atatacagac ttgaaacaca ttcttgcttt agctaaaaat caacaagaat caaatgattc
    37801 tctaataaag aaagaactta tttttacgaa aaaaatgatt aatttcataa aaccagctga
    37861 taagcaaact acaacttata attcttatgg aaatattaga aaataaatat taggaggaga
    37921 aatatggcag gattattttc cattttaaat acagctaatt caggaatgaa tgcgcaccaa
    37981 aaagcaatac agacaatttc tcataatata agtaatctag atacagacgg ttattctagg
    38041 caaaggaccg agtttgccac aaatagtcca atgtatatgc ctagtttgag taattcaatt
    38101 gcaagaggac aattagggac tggagtacat gtaacggatg taacaagagc tagaaatagt
    38161 ttttatgatt accaatttag ggcagaagct cataagtatg gtaagacagt ttcaaaatat
    38221 gattattaca atactataga aactatacta aatgaaccat cagactatgg aatatctgca
    38281 ggtatagatg atttttttaa aggatgggag tcattatcaa aggatataaa tagtgacagt
    38341 aaaaaaagtc tagtggtaga aaacgcatct aatttagcaa ctttaataag tgaaagttat
    38401 aagaagcttg ataagttaaa agaagatata gattctaata tagacactga gataaaacaa
    38461 ataaatgaca tgttaaaaag tcttgaagat ttaaataaat ctattgatat tatatctggt
    38521 tctggctcta caccaaatga tttacttgat gaaagagata gaattttaga taacttgagt
    38581 tttaaactag actttgaaaa tagtgatgtt aaaaatatgc ttgcagatgg taaattagaa
    38641 cttaatgaat taaaaaatcc agatggaact tggaaaacag gtatatcagg gaccttacaa
    38701 ggcttgtttg aaatgcatgg taagattgat acatataaaa gcgatttaaa agatgtatct
    38761 gatggattag ctaaacagat aaatgatgtt tataatacgt ctgcaggaat aacagtaaca
    38821 gattttttta taacttcaaa tgtagatggt gaagatatta taaagataaa tccagctata
    38881 aagagtaacc caaatgaatt aaagcttact gctgatgaag cttcaaaaat tgctaagtta
    38941 aaggatgaaa aaatagatat tggggtggct ggtggaaaag taagtacaat aagtgaccac
    39001 tataaagcct ttgcagaatc aatcggttta gattctcaaa aagtaaatca agatgaagca
    39061 aatcaacgta aaattataaa taatattgat aactcaagaa tgagtgtttc aggtgtatct
    39121 ttggatgaag aaatgacaga gcttataaaa atacaacgtt catatcaagc tagtgcaaaa
    39181 gttatgtcaa cagctgtgca actactggat gttgtaataa atggaataat ttaatagaag
    39241 ggggaattta taatgagagt cagtacaggt atgatgtcta gcagctatct aaactcacta
    39301 caagataatc tacaaagatt ggataaggtt aatagacaag ttaatacaac aaaagaaata
    39361 aacaagctat cagataatcc atataaagca ataaagatat taaactcaaa aagtgaaata
    39421 aagactatgg agacttatgt tgagaattgt aaagatacag ggaattggtt agaaactact
    39481 gatactgcat tagatcagct tggaaaatta ttatcagaca taaagaaaac tttggtggca
    39541 agtggtgatg gtgcatactc agatgatgaa ttaaagactc ttagtaatga aactaatgaa
    39601 aaaatgaaag aaattgcaaa tgctttaaat gcaacacatg aagggaagta catattttca
    39661 ggttcaaata cgggggtacc acctgttgaa tgtatagata atgctgatgg ttcagtgagt
    39721 ttaaagttta attctagtct aaatcttaat aaattaaatg actctttgag tatggatata
    39781 gctcaaggaa taagtgtaga ttataacgtt aaattgagta gccttggatt tgacccaaca
    39841 aaaactgacc catttgaaga gttaaataac atatcaaaaa aattagcaaa tccagatgct
    39901 gatgctataa aagagcttac aacaacttgt ttgggcgata tggaacaact aatagaaaat
    39961 acagtaaatg ttaggtctat atatgggaca aaagcaaata cagtagaggc tatgaaggaa
    40021 aaaaacgatg atagtttgat acagttaaag gatattttgt cacaaaatga agaaatagat
    40081 tatggtgaaa aacttgttca attaaaggca gcagagttaa cttatcaagc ttcactacaa
    40141 acaggaggaa agttatttaa tgtatctata ttagattaca tataataaaa taacgcagat
    40201 atgatgaagg ttacattaaa aaaaggtata ttaggttttg aaaatttaaa agaatatgag
    40261 cttttagata tagaagataa tgattattta aaagaattta attctactga agaagaaggt
    40321 ataggatttg ttgttgtatc tccatttgag attatcaatg aatatgaaat agttttaaat
    40381 caggaaacaa tagaaaaact agaagtgaaa tctccaaagg atgttgtatt atttaatata
    40441 attactcttg gtcagacatt tgaagaatca actgtgaaca tgaaagctcc tatagtgata
    40501 aatataaaaa ctaattttgg aatgcaaata attttacagg atgataaata ttcaataagg
    40561 catccattat tgagaggtga tagtggatgc tagtaatttc aagaaaaaaa gatgaagcag
    40621 tacttatagg agaaagcata gaagtaaaaa ttgtaggggt agatggaaac aatgtgaaac
    40681 tagctatatc agcgcctaac aatataagta tattaagaaa agaaatatac gaaaaagtaa
    40741 agagtgagaa tataaaagct acaaataaaa atataaaaat tttaaagtca ttaaaataat
    40801 aagcttggag gaagtaatat atgtatggag aaaatcctta taattcttat aaacaaaatg
    40861 ctgtttttat ggcatcaaag gaacaattgt tacttatgct tgttgatgga gcagtaaaat
    40921 ataccaaaat agccaaaaca gctatcttag ataaaaatac acaaaaagcg catagagagc
    40981 ttataagagt tcaggatata tttacagaac taatggttac attagaccaa aatgcaggtc
    41041 aatgggcaaa ggatatgtac agagtttatg attttgtaag atatgaactt tcaagagcta
    41101 atattagaaa agacattcaa ataatagata atgtgttgcc tgtaatagaa caaattaaag
    41161 atacttggca tgaagctgat aaaaaaagta aagaagaaag aagtaggtac aaataatgga
    41221 attaaattta gctgaattag ataagttatc aaaagaggaa ttactattga tgcttgtaga
    41281 tggaacagta aaatatacca atatatccaa agaagctctt ttaaataacg actatttgaa
    41341 agcacataat gaacttgtaa gagttcaaaa tatatttaca gaactaatga ctacattaga
    41401 ccaagatgca ggtcaatggg caaaggatat gtataaagtt tatgaattta taaaatctga
    41461 attagcaata gctgatgaaa ataaagatat taaaataatt gatgatatat tgccaatagt
    41521 aaaacaaatt agagatacat ggcatgaagt ttacaatcaa ctaaaaatat aattggatgg
    41581 agggataaag gtatgtcaag tataagtcct ataagagtta caggtctttc aggaaatttt
    41641 gatatggaag gcataatcga agctagtatg attagagaca aggaaaaagt tgataaagca
    41701 aaacaagaac aacaaatcgt taaatggaag caagaaatat atagaaatgt tatacaagaa
    41761 tcaaaagatc tttatgataa atatctaagc gtaaattctc ctaatagtat agtaagtgaa
    41821 aaagcatact cttctacaag aataaccagt tctgatgaaa gtattatagt agcaaaaggc
    41881 tcagctggtg cagaaaaaat aaattatcaa tttgcagttt ctcaaatggc tgaaccagca
    41941 aaatttacta ttaaattaaa ttcaagtgaa cctattgttc aacagtttcc tccaaatgct
    42001 agtggagcga gttctttaac tataggagat gtaaatatac caatatctga acaagatact
    42061 acaagtacta ttgtaagtaa gataaactcc ctttgcgcag ataatgatat aaaggcttct
    42121 tatagtgaga tgacaggtga attgattatt tcaagaaaac agactggttc gtcatcagac
    42181 attaatttaa gagtaattgg aaatgacaat ttagctcagc aaattgctaa tgataatggt
    42241 atcacattta taaatgatgc tggtggaaac aaagtggcaa atgtatatgg aaagaatcta
    42301 gaagctgatg taactgatga acatggaaga gtaactcata taagtaaaga acaaaattca
    42361 tttaatatag ataatattga ctataatgta aattcaaaag gaactgcaaa gttgacttct
    42421 gtcactgata ctgaagaagc tgttaaaaat atgcaagcat ttgtggatga ttataataaa
    42481 ctgatggaca aggtctatgg tttagttact actaaaaaac caaaagatta tccgcctctt
    42541 acagatgccc aaaaagaaga tatgacaact gaagaaatag aaaaatggga aaagaaagct
    42601 aaagaaggta tacttagaaa tgatgatgag ttaagaggtt ttgttgaaga tattcaatct
    42661 gcattttttg gagatggaaa aaatattatt gcattaagaa aactaggtat caatgaaagt
    42721 gaaaattaca ataaaaaagg tcaaatatca tttaatgcag atactttttc aaaggctctt
    42781 atagatgata gtgataaggt atacaaaaca ctagcaggtt attcttcgaa ttatgatgat
    42841 aagggaatgt ttgaaaagct aaaagatatt gtatatgaat attctggaag ttcaacttct
    42901 aaacttccta aaaaagcagg tatagaaaaa actgcttctg ctagtgaaaa tgtatattca
    42961 aaacaaattg cagagcaaga aagaaatata agcaggttag ttgaaaaaat gaatgataaa
    43021 gagaaaagac tttatgctaa atattcagcc ttagaatctt tgttgaatca gtattcttcc
    43081 caaatgaatt atttctcaca agcacagggt aattaattat ggataaatta aaagaaaaat
    43141 taaatttata taaagatata tctttacaaa ttataaattt gattgaaaag gaagaatata
    43201 taaagataag tattaagtta ggtgaaagac aagagattat aaatagtgta agtgaaattg
    43261 atagaaatga ttttatacag ctttataata gaatggaatt aattgaaatt gatagcagga
    43321 taagagatat tttacaagga caattattag aagtaaaaaa agaattacat gagtataagc
    43381 ttactaaaca agtgaacaca atgtattata acttaaatag agaaaaagta aatattttta
    43441 ataaaaaagt ataaagttat gtaccatctt gtccgattat ataaatgaag aattaataaa
    43501 aaagattaaa agtagaaatg acaaggatgt caactatact aaggagggta aaataatgag
    43561 agttaataca aatgtaagtg ctttgatagc aaataaccaa atgggaagaa acgtaaatgc
    43621 acaaagtaag tctatggaga agctatcttc tggtgttaga attaagagag ctgctgacga
    43681 tgctgctggg cttgctatat ctgagaaaat gagagctcaa ataaaaggtt tagaccaagc
    43741 aggaagaaac gttcaagatg gtatatctgt tgtacaaaca gcagaaggtg cattagaaga
    43801 aactggtaac atactacaaa gaatgagaac tttatcagta caatcttcaa atgaaactaa
    43861 tactgctgaa gaaagacaaa agatagctga tgagttacta caattaaaag atgaagttga
    43921 aagaatatca agctcaattg agtttaatgg taagaaatta cttgatggaa gttctacaga
    43981 aataagatta caagttggag caaactttgg aacaaatgtt gcaggaacat ctaacaacaa
    44041 caatgaaata aaagttgctc tagtaaatac ttcaagtata atgagtaaag caggtataac
    44101 aagttctaca atagcaagct taaatgcaga tggaacttct ggaacaaatg cagctaaaca
    44161 aatggtatct agcttagatg tagctcttaa agaattaaat acatcaagag caaaattagg
    44221 ggcacaacaa aatagactag aatcaactca aaacaactta aataatacta tagaaaatgt
    44281 tacagcagct gaatcaagaa taagagatac agatgttgct tcagaaatgg ttaacttatc
    44341 taaaatgaat atattagttc aagcatcaca gtcaatgctt gctcaagcta atcaacaacc
    44401 acaaggagtt ttacaattat taggataaaa taattttgta tattggatat agaagaaagg
    44461 gtaaggcttt gctttactcc tttctttttt tacaaatata cttttgaaag gaaaatttat
    44521 atgttactta gtatagttat gattgttaaa aatgaagaaa aaatacttga aaaaacttta
    44581 aaatcattaa ccacattaag aaattctata gaaagtgaac ttataatagt cgatacaggt
    44641 tctacagatg atactataaa aatatctaag aagtacacgg aaaaagtgta ttttcataat
    44701 tggaataatg atttttctag tatgagaaat atatcaatat cctatgctaa aggtgaatgg
    44761 ttacttatat tagatgcaga tgaaatacta attgattctt caactattgt aaaatttttt
    44821 aaagatggtc ttgataaaaa atttaattct gcttcaatac aattaaaaaa tctttactca
    44881 tatgataaaa aattatatgg atattgttca gtgcttagac tttttaaaaa cgttgatttt
    44941 agataccatg gtaaagtaca tgaacaacca atatacaaaa accctatatt taataatgtt
    45001 gctgattttg aacattatgg atatttgttt gaagatgaag aaataagatt aaataaggtg
    45061 aaaagaaatg aaaaactatt atttcaagag ttaaaagaga atgataattc tccatataca
    45121 aactatcaat tagggaaaaa ttttattata ttaggaaaat atcaggaggc tttatactat
    45181 cttgagaaat cttatgaatt atattctaaa ctagaattgg cacctggtta cttgataaca
    45241 agccttgtaa aaacatattt atatttagga aaacataaga agtgtgaaag attgtgctta
    45301 aagtatatta agaaagacat gaataatatt gatatatatt attatattgc acaagcacaa
    45361 gtagatttag gaaagtatga aaatagtata gattcgtata agagatatat ttacttgtta
    45421 gataattatg aaataagtac acaagcaaat agtttatttt cagacactga tactgtaggg
    45481 cttagagatg aggctattat tacactgata aagatttatt ataagcttga aaaatatgag
    45541 tttgtaattt cagagtataa taacattgaa gatgtggaaa agaaaaagaa tgtgtacttt
    45601 agcttattta tgtcactata taaattgaat agatttgaag atataaaaaa ttattataaa
    45661 gaagtttcaa attctaaaat tgaaaaaaat agtttttata gaaatataga agaagttata
    45721 aataatatta aagaggatga aaaagagtat gtatataaaa tcctttgtga cattgaaggt
    45781 aattatggca ttttaaatca aataaggata aataaatgtg tatctataga taaatgtaaa
    45841 gaaatactca atgaagaaaa ggaaataatt tatgcatata ttataaaaat agcttttgaa
    45901 gagaagattg atttactaga tattttttat gaaatggaat atgtatggat agagaaatat
    45961 ctaaaatatc ttttagcatt agaaagaagt tttaatttag tactatataa gtacataatt
    46021 agtcgtccaa atacatcaga tatggaaaag ataagagtat ataaagctat atctaaagta
    46081 gttttagaaa gtataagctt aagtgatgaa aaatataagg agatttttta tctttatata
    46141 atgtattctt ataagtacat aagatacata tatagcaatt taagtgattt tgaattaata
    46201 aaatttgttt gtaatgattt agataagttc actatagaat ttaagcatct acttgatatt
    46261 aaaaataaag taaataatgc taatgaaaaa ttagagtata tacatagtat gaggaaatta
    46321 ctaaatgagt atccttttta taataaaata ataaaaatac ttataggtga attagaagaa
    46381 aatataaaag aaagtgaaga atttaaggat ttaaaagata gttttattat tagtatagaa
    46441 aatatgattg aaactggcaa aataaatgat gcgaaattat tggtagatga ctattcaaaa
    46501 acatttgtgg atgatataaa gattttaaat ataaagggaa ttttatacat gtttgaaaat
    46561 aagcttgata aagctgattt tatgttaaaa aaggcattat cattagatgt agaaaatgaa
    46621 gatactatat ataatataga atacttaaaa agtttaagat aagtcaaaaa taggagaatt
    46681 gaagaatgaa tataccaata gtggttaatg aaaaatctaa agacataata aaagattggc
    46741 aatatacatg tacaaatgaa gatataaaat ttacaagcat aataatattg acatataatc
    46801 aaatagaata tactaaatta tgtattgaaa gtattaggaa atttacccca aagaataaat
    46861 atgaaataat aataattgat aataactctt ctgatggaac agttgagtgg ttaaagggac
    46921 aatctgatat aaaatctgtg tataataaca aaaacttagg atttccaaaa ggatgcaatc
    46981 aaggtattga gttagctact ggtgaaaaca tattgttgtt aaataatgat gtaatagtta
    47041 ctccaaattg gctctataat ttgaataaag ctttatggtc tcaagataat ataggtgctg
    47101 taggacctat tacgaatgca tgttcatatt atcagcaaat tgatgttgat tataagtcaa
    47161 ttgaggacat gtttatattt gcacacaaaa taaataaatc taatattaac ctatggaaat
    47221 atagattgaa gttagttgga ttttgcatgt taataaaaaa aactgttctg gataatgtag
    47281 gggttttaga tgaattattc acacctggaa attttgaaga tgatgattta tcttttagga
    47341 tgatagagtc aggatataag ttattattat gccaggatac ttttatacac cattttggaa
    47401 gtgtgtcttt taaagaaaat cgtaataaat atacagatat aatgtatatt aatagaagaa
    47461 aatttgaaac taagtgggga tttaatccag aatatagtgg aatgataaga acagatttga
    47521 tttctaaaat agatgatagt ttggaggcaa atattaatgt tttagaaata ggatgcggta
    47581 taggggctac tcttttagaa ataaaaaata tatataaaaa tgcatcaata tatggaatag
    47641 aaattagtga gtcagcaggt aaacttgcaa agtatagtgc agatgtactt atagcaaata
    47701 tagaaaaagt aaaattggat tataaaaaag atttttttga ttatataata ttgggagatg
    47761 tacttgaaca tttgattgac ccatggaaga tagtcaatga attgaaacaa tatctaaaga
    47821 aggatggtta tctggtatct agtataccaa atattatgca tgtttctgtt ttaagaaatc
    47881 ttgtgttagg taattttaca tatgaagatt caggaatttt ggacaagact catttaaggt
    47941 tttttacatt aaatgagata acaaaaatgt ttaatgatag taattacata attaatagta
    48001 ttacagctac tagtatgcca ctttcggaaa ttgataatag cttcattgat acaatatgta
    48061 aggaaagctg taaagaaatg agacaacagt acgaatctta tcaatatata gtaaaagcct
    48121 ctaaaatcct agatactaaa agatattcta gtattagtat gattaattta aaatacgctt
    48181 taatgagaat tgataatgaa ttagatgtag atagtaattt aagttatata tttgataatt
    48241 ataatataac tgaaccatgt tttgttgatg atattgaata tctaattgat acatttgtga
    48301 taaataaagc aactctatta aatagattag gagttgaggc atttaacaga ggtctaggag
    48361 attttagtat aaacatgttt atgacagtat tagagatgga tagagatgat attgactcaa
    48421 tatataatat aacaagtgta ttatgtgaat tagaagaatt tgatactgct tataatttaa
    48481 ttaaaaatag tagcgaaaaa gtacagagtg aaagtgatat acaagagata ctttgtttta
    48541 ttgaggggaa gaaatatgaa taatgataag atatgtttta taacctgtgt aaatgatgaa
    48601 aggctttatg aagaatgtct tgtatatata aatagcctag acattcctaa aaactttagt
    48661 atagaaacag tagctttaag aggagccgat tctattgcct ctgcttataa taaagctatg
    48721 agagaaacag attctaagta taaggtatac ttacatcaag atacttttat aattaataaa
    48781 aatttcatac atgatatttt agagatattt aaaaaaaata cagatgtcgg aatgattggt
    48841 gtagctggag taaaagatat gcctgtgaat ggaatttggt gggattctga taaaaaagca
    48901 gggaaagtat atgatagtca tacaggtaaa atggatattt atgaatttag taactttgaa
    48961 gaatgtttta atgaagttat ggctatagat ggattaatta tgattactca atatgatgta
    49021 ttttggagag aggataagtt tgatgggtgg catttttatg atttgtcaca gagtatggag
    49081 tttagacaaa ataaatacaa agttattgta ccaaaacaaa ataagccttg gtgtatgcat
    49141 gattctggag tagtaaatat aaataatgga tatgatgaat ataggataaa atttgtaaaa
    49201 gagtacataa atcttgattt atatagagaa aatttttatg aatttgataa gaagaaagca
    49261 agtaccatac ttaagagtaa taagcatgta atggattgta tgaaagagga gcttacagaa
    49321 caattaagat taaagaatta caaggatgtt ttaggtattg tttctaaaat tgctcatttt
    49381 tctgttttta atcatactgg tttgtattct tgtgaagaat gtgagagagc attgctagaa
    49441 tgtgcaaata atttaccaga aatagaaaat acgaaattat ttacaaaaaa agatttaaaa
    49501 agaagaaaag tattacatgt tttaagtgaa ggatattcaa caggaggtca tacaagacta
    49561 gttaaaaatt ggattgaaat tgacaaagac tctgttcatt cagttgtaac tacatggcaa
    49621 tcagatacac tgccagactg gttattaaaa gcaataaatg atagtggtgg cgaagttata
    49681 tctttggatt taatttcaga tgattattta aaaagagctg tagctttgag gaaaatagct
    49741 tatgactggg ctgatatagt tattttatat gttcatatgt gggatacaat acctgttgtt
    49801 gcttttggag ttgatgggaa aatacctatt ttacatgtta atcactatga tagtactttt
    49861 tggttaggga caagtattgc agatattgtc attgatttta gcgaaactag tcaattaatt
    49921 acattagata aaagaggcat agataaatct gaaatattac caatgccatt aaaagaaaaa
    49981 gaagtatttt caaaatctga agttagaaaa cgatataatt tagaggatga tgtgacaata
    50041 atattgacta ttgctagtga aagaaaatac aatagtgtaa atgatattaa ttactttaaa
    50101 attgctaaaa cattgattga aaatacagat aatacaatta ttattgttgt aggagttgat
    50161 acagagaatc tctattggaa aactttaagt aatgttacag ataatagagt tatatgtttt
    50221 ggaacaacag caaatataga tgatttttat aagatttcgg atatatacct agagtctttc
    50281 atggtaggaa gtcatacttc aaaattagat gcaattttat atgatttaat tcctgttaag
    50341 tttaaaaatg aagtttcacc aatttttaca aatatgtgga attttataga taagtttgat
    50401 tataagaata taaaagaggt tatagacatg ataagaaggt acaatactag tgaatttgac
    50461 agggataaga taaaagcaaa acaatcggaa ataaaacatt ttgttataga taaacattgt
    50521 attgaaataa aaaactatat agacaaaata tataatgaag ttaatcatca taaagttaag
    50581 tatgatttat ataaaaatga tgaaatcgag gactatcagc tttttgtagc attatatggc
    50641 tatatggtaa aaaataaaaa catgtcatag gtggtaaaat gaacaatatt catatcaata
    50701 aatttgaaaa aagctgtgaa aaaaactggg attattttgt agaaaataaa tctataaatg
    50761 gtacatttct gcaaactaga aatttcctta attatcatga ggagggaaaa ttcactgacc
    50821 actcattaat aatatgtaaa ggtgaagaaa tactagcagt cgtacctgca tgtgaaactt
    50881 atgaagatgg tgaaagaata tttttttctc atttagggag tacttttggt ggcattataa
    50941 taagaagtgg ttttaatgat attaggcatg taaatgccat tacaaataag ttagaaatat
    51001 atttacagga acaaaatttc gataaagtat atttaaaaaa tacatcaaga atattttgta
    51061 aagaggatac ttcattacta gaatattttt tatttaagaa taattacatg ttttatgatg
    51121 aattaagtat gtttttagaa ttaagtaagt ataaagaaga tatattagcc aattttaaat
    51181 acagtaaaag aagagatttt aaatattctt tgaagaataa tcttaccttt aaaagactgt
    51241 atagtgatga agatatagtt tgttttttta atttactttc taattgttta gatagtaaag
    51301 gagctaaagt aatacattca gtagaagaac ttttggagtt taaaaataat agattaaaag
    51361 atattgtaga attttatgca gtatatattg atgacattat gatttgtggg agcatggtat
    51421 ttaactttca agaaagagtt tatcatactc aatatctagc atcaaattcg acatataata
    51481 aatattatcc aatgatattt ttatactata atctaataaa agatgcttta gagagaaatt
    51541 ttaagtatct ttcttttgga attagcactc taaatagagg caaatactta gatgagggtt
    51601 tagctacatt taaagaaggt tttggttcaa gtgcaatttg taacagaaca tactataaaa
    51661 atataaataa gtgagaatca tgttttatag agtttttaga gtatagtaaa tatctagtac
    51721 tataatatta cataaaaata attagtttat aaagaaggtt tgtatatgaa aagattaatg
    51781 attttaggct caattaagta ttttgagaat attgttatgt cagctaaaag agagggaata
    51841 tacacaattg tttgtgataa tagaattgat actcctgcga aaaagatttg tgatgaagca
    51901 atagatgttg atgtatttga ttttgataaa ataaagaata tagctataga taaaaagata
    51961 gatggaattt taactggatt tacagattct ttaatgaagc catatgttta tattgcaaat
    52021 gaactaaatt tgccatgtgt gatttcttgc aatcaacttg aatctgtaac taataaggct
    52081 gttatgaaaa attattttaa aaataatgga atacctacaa ctgattattg tattattgaa
    52141 agtttgaaag atttagaaaa aatacaagga ttaaattttc cacttgtaat aaaacctgtt
    52201 gatggttatg gttcaagagg tgtatatttt gtatcaaaca tagaagaatt aatagaaaaa
    52261 tttgaatatt ctaaggaagc ctcgcaagat ggacaagtaa taattgagga attttatgaa
    52321 agtgaggaaa tacaaggact agcttgggtt cataatggag agtcacatgt attttatatt
    52381 ggagatagag agcttgtaaa tatacataca ggaagagctg gaaaaccaga tagattaatt
    52441 tatccatcaa agtattgttt tcaatatgaa gaagaaataa aatccatata tcaaaatata
    52501 acgcaggctt ttggcataaa aaatggacct ctttatgtac aaatgttagt gggagcatat
    52561 ggaataaagg tatctgaagt aatgccaaga ttacctggag gttgtgacta tatggctatt
    52621 tctcaaataa cagatttaga cataggaagt ttatttgcaa actttagtgt gaataatgaa
    52681 gtaaattttg atgaaataaa gaaatataat atgcatctat caaaatgtgt atatgcatta
    52741 ccaatataca taagacctgg cacaataaaa aagataaata atatagataa aatagaaaac
    52801 ttagattatg tcactcaatt tctattacta gtaaaagaag gagatacaat aggattatat
    52861 gaagatgaga gacaggattg tggaagagtt tatggagtag caaataatgt atttgatgca
    52921 aatataaaga agaaatgtat tcatgaaatg atagaaatac ttgatgaaaa taatgaaaat
    52981 atgatagaaa atttttaaat aagggagtat gatagatatg aagctaataa ctcatgaaca
    53041 aattatgtca ttaaacattt ctcagaaaca aggatacgag tggataaaag aatccttttt
    53101 aacaaaaaaa gattgtcaat taccaccaaa aataagcatg gtagaagaag ggcatgtatt
    53161 ttacaatgta atgccgtccc taaatcataa tcataagatt ggtggaataa aaatggttac
    53221 aagattgcca gggagaaatc caagtttaaa ttcagagata tttctatata atatggaaga
    53281 tgctaaatta aaggctattt tagatggcaa ttatattaca gcttttagga ctgctgctgt
    53341 tgctgccttg gctatcgata catttgctaa aaaagatttt aatactattg ctattatggg
    53401 tttaggtgta actgcttctg ctataatgaa tatgctttca tatactctta aagataaaaa
    53461 tataaatata aagttactta aatataaaga ccaagcagag tcgtttgtaa aaaagtatga
    53521 aaaaaatgaa caatttagtt ttgaaatatg tgacacacat gaagaactta ttgtagattc
    53581 agatgtagtt atttctactg tgacatatac agatgaatat ttatcagaat ttaaatggtt
    53641 taaaaaaggt gctttattat tacctgtaca tgttatggga tttggaaatg ttgattatct
    53701 atttgataaa gtatatttgg atgattataa tcatttgaaa catctagaaa atatagatag
    53761 atataaatat tcttgtgaat tttatgatgt tttaagtaaa caagctaaag gaagagaaaa
    53821 tgatgatgag agaataatat cttacaatat aggtttgtca atacatgata tattatttgc
    53881 aaataaaatt tacgaattgt gtgaaacact aagctgtgaa gaaatagaat taaaagaacc
    53941 aaaagaaaaa tattggatat agtttatttc attcatattt acaatagttt attgtaaata
    54001 tgaatatgaa gtaatatatt gttagaaaat tagcatgaat aggatagata aatttatgga
    54061 taaatataat ttaattaggt tcgtatctat tggaaacgaa tatataggaa atttagttgc
    54121 attagaatca aataaagcaa taccttttga tataaagaga gtttattaca tatatgatgt
    54181 accaaacaat gtaaagagag gatttcatgc acacaaaaac ttggaacaag tactagtttg
    54241 cctaaatgga agtgtgaaga taaaatgtta tgatggtgta aaagagacta tatttaattt
    54301 acataaaaat gatgttggat tatatatagg aacgggagtg tggcatgaga tgtttgattt
    54361 tgatgaaaaa actgttttaa tttctatagc atctgaacat tataatgaag aagattatat
    54421 tagagactat aaagaatttt taaagtattt aaataatgtg tagttattat acaattagtg
    54481 atgtattttc ttaattaaat aaatttaaga tagccacata gagaatagat agattaggat
    54541 gtgaatttat tgttggttaa atttaataat ttattaaaac agttccaaac tcataaagaa
    54601 gaatatgaac agaagattaa ggatgtaatg gaaagtggat attatatcct tggaaatgaa
    54661 gtaaagcatt ttgaagaaga atttgctaac tatgtaggta gcaaatattg tgttggttta
    54721 gccagtggtt tagatgcact gataattgct tttagagcat taggaataag caaaggtgat
    54781 gaagtaatcg tgcaatcaaa tacatatatt gctagtgtta tgggaatcag tataaatgga
    54841 gcaacaccag tatttgtaga accaaatgaa tattataata tagattcatc taagatagaa
    54901 gaaaagataa atagcaaaac aaaagctata ttagtagtgc atttatatgg tcaagcaagt
    54961 aatatgaaag atatacaaga attatgtgat aagtataact tgtatttaat tgaagattgt
    55021 gctcaatctc atggagctaa atatgatgga aaaatgacag gaacatttgg tgatataggt
    55081 tgttttagtt tttatccaag taaaaatttg ggtgcatttg gagatgcagg agctatagta
    55141 actagtaatg aggaaatagc tgataaagtg aaaattctta gaaactatgg ctcagaaaag
    55201 cgatattata ataaatttgt cggatataat tcaagattgg acgaaattca ggcaggttta
    55261 ttaagagtaa aacttaaata tctaaatgaa attgaaattg aaaaatcaaa aatcgtagat
    55321 agatatctta atgaaattaa taatcccaaa gtcatacttc caaagataga aaataattgt
    55381 actcatgtat ggcatttatt tgttttacaa gttgaaaaac gagatacatt tatggaatac
    55441 ttaaagaaaa gtggtataga gtctataata cattatccga taccaccaca tctatctgaa
    55501 gcatatgctt atttgggata taaaaagggg agttttaata tagctgagaa ctattcagat
    55561 aaagttgtta gtataccttt atttattggt atggaagatg aagaagttag ttatataatt
    55621 aaaattataa atgaatatta aactactcat aataatatca aaaataaatt ttttatatat
    55681 catttattta aatttcgtta attgacattt ttaaatattg atatattgta caaataaaat
    55741 tgaaatatat ggaaaaatat agaattaaga tga
//
LOCUS       DAMJWS010000015        54444 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMJWS010000015 DAMJWS010000000
VERSION     DAMJWS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.015"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            479..1318
                     /locus_tag="PC399_000987"
     CDS             479..1318
                     /locus_tag="PC399_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF2591862.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1315..2496
                     /locus_tag="PC399_000988"
     CDS             1315..2496
                     /locus_tag="PC399_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591863.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2800..3702
                     /gene="ptb"
                     /locus_tag="PC399_000989"
     CDS             2800..3702
                     /gene="ptb"
                     /locus_tag="PC399_000989"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF2591864.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3724..4803
                     /gene="buk"
                     /locus_tag="PC399_000990"
     CDS             3724..4803
                     /gene="buk"
                     /locus_tag="PC399_000990"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2591865.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4793..5470
                     /locus_tag="PC399_000991"
     CDS             4793..5470
                     /locus_tag="PC399_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2591866.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            5513..5728
                     /locus_tag="PC399_000992"
     CDS             5513..5728
                     /locus_tag="PC399_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2591867.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5757..6836
                     /locus_tag="PC399_000993"
     CDS             5757..6836
                     /locus_tag="PC399_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2591868.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6836..7588
                     /locus_tag="PC399_000994"
     CDS             6836..7588
                     /locus_tag="PC399_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2591869.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7589..8146
                     /locus_tag="PC399_000995"
     CDS             7589..8146
                     /locus_tag="PC399_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2591870.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8316..9662
                     /locus_tag="PC399_000996"
     CDS             8316..9662
                     /locus_tag="PC399_000996"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF2591871.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9929..11761
                     /gene="glmS"
                     /locus_tag="PC399_000997"
     CDS             9929..11761
                     /gene="glmS"
                     /locus_tag="PC399_000997"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF2591872.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12212..13507
                     /locus_tag="PC399_000998"
     CDS             12212..13507
                     /locus_tag="PC399_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF2591873.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADNENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLIKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFTSNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNRQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            13957..14694
                     /locus_tag="PC399_000999"
     CDS             13957..14694
                     /locus_tag="PC399_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF2591874.1"
                     /translation="MKRVKLAISFFILMIIGMTISYADTSINTKYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGRTLIYIATPIIKLDY"
     gene            14715..15968
                     /gene="murA"
                     /locus_tag="PC399_001000"
     CDS             14715..15968
                     /gene="murA"
                     /locus_tag="PC399_001000"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF2591875.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            16055..17119
                     /gene="spoIID"
                     /locus_tag="PC399_001001"
     CDS             16055..17119
                     /gene="spoIID"
                     /locus_tag="PC399_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF2591876.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKAESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            17185..17853
                     /locus_tag="PC399_001002"
     CDS             17185..17853
                     /locus_tag="PC399_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF2591877.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            17951..18211
                     /gene="spoIIID"
                     /locus_tag="PC399_001003"
     CDS             17951..18211
                     /gene="spoIIID"
                     /locus_tag="PC399_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF2591878.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            18408..19427
                     /locus_tag="PC399_001004"
     CDS             18408..19427
                     /locus_tag="PC399_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2591879.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDNRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            19443..19871
                     /gene="fabZ"
                     /locus_tag="PC399_001005"
     CDS             19443..19871
                     /gene="fabZ"
                     /locus_tag="PC399_001005"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF2591880.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRRNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(19982..20491)
                     /gene="yyaC"
                     /locus_tag="PC399_001006"
     CDS             complement(19982..20491)
                     /gene="yyaC"
                     /locus_tag="PC399_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2591881.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      20680..20783
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20919..22112
                     /locus_tag="PC399_001007"
     CDS             20919..22112
                     /locus_tag="PC399_001007"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF2591882.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEDTKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            22427..23524
                     /locus_tag="PC399_001008"
     CDS             22427..23524
                     /locus_tag="PC399_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591883.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMTIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            23788..26007
                     /locus_tag="PC399_001009"
     CDS             23788..26007
                     /locus_tag="PC399_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF2591884.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIANKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHVEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            26010..26792
                     /locus_tag="PC399_001010"
     CDS             26010..26792
                     /locus_tag="PC399_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF2591885.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKIVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKDNVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            27124..29037
                     /locus_tag="PC399_001011"
     CDS             27124..29037
                     /locus_tag="PC399_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2591886.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIGGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMFTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSSNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIRIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            29178..29486
                     /locus_tag="PC399_001012"
     CDS             29178..29486
                     /locus_tag="PC399_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2591887.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            29510..29836
                     /locus_tag="PC399_001013"
     CDS             29510..29836
                     /locus_tag="PC399_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2591888.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            29875..31209
                     /gene="celB"
                     /locus_tag="PC399_001014"
     CDS             29875..31209
                     /gene="celB"
                     /locus_tag="PC399_001014"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF2591889.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGSDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQVVLLILGIFMYIPFIKMLDKQYLREENATVEEEDDDISFDDLVL"
     gene            31220..31738
                     /locus_tag="PC399_001015"
     CDS             31220..31738
                     /locus_tag="PC399_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF2591890.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGIMMEQFLNE
                     SKVIATLFCGDEFFVNNEEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            31834..32793
                     /locus_tag="PC399_001016"
     CDS             31834..32793
                     /locus_tag="PC399_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF2591891.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            32842..33894
                     /locus_tag="PC399_001017"
     CDS             32842..33894
                     /locus_tag="PC399_001017"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2591892.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            33897..34571
                     /locus_tag="PC399_001018"
     CDS             33897..34571
                     /locus_tag="PC399_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF2591893.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            35124..35675
                     /gene="raiA"
                     /locus_tag="PC399_001019"
     CDS             35124..35675
                     /gene="raiA"
                     /locus_tag="PC399_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2591894.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            35807..38482
                     /gene="secA"
                     /locus_tag="PC399_001020"
     CDS             35807..38482
                     /gene="secA"
                     /locus_tag="PC399_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2591895.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            38497..39604
                     /gene="prfB"
                     /locus_tag="PC399_001021"
     CDS             join(38497..38571,38573..39604)
                     /gene="prfB"
                     /locus_tag="PC399_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF2591896.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNKQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            39748..41889
                     /locus_tag="PC399_001022"
     CDS             39748..41889
                     /locus_tag="PC399_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF2591897.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLEKLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMPPHRVL
                     AINRGEKENFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DNEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKTIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNICEIDEKIKEIGLRELSEKLEVGQVTLKDIITEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            42037..43203
                     /locus_tag="PC399_001023"
     CDS             42037..43203
                     /locus_tag="PC399_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2591898.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      43263..43380
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            43504..44109
                     /locus_tag="PC399_001024"
     CDS             43504..44109
                     /locus_tag="PC399_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2591899.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPFVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVILSLVTLPLYKKMGNVIKK"
     gene            44438..45478
                     /locus_tag="PC399_001025"
     CDS             44438..45478
                     /locus_tag="PC399_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2591900.1"
                     /translation="MNLSKKIKKLFLDDKGYYFNSNWKDIHIGIYNTKLGDSLKYITN
                     EYIENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYICLEAIKKDCNALKYVRNKTERLIIKVIRHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVENQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            45617..46069
                     /gene="tsaE"
                     /locus_tag="PC399_001026"
     CDS             45617..46069
                     /gene="tsaE"
                     /locus_tag="PC399_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF2591901.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDDGREMILTPKGEFYKEIVEELIK"
     gene            46069..46785
                     /gene="tsaB"
                     /locus_tag="PC399_001027"
     CDS             46069..46785
                     /gene="tsaB"
                     /locus_tag="PC399_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF2591902.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            46775..47251
                     /gene="rimI"
                     /locus_tag="PC399_001028"
     CDS             46775..47251
                     /gene="rimI"
                     /locus_tag="PC399_001028"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF2591903.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            47257..48273
                     /gene="tsaD"
                     /locus_tag="PC399_001029"
     CDS             47257..48273
                     /gene="tsaD"
                     /locus_tag="PC399_001029"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF2591904.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKKGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKEKGYNIITLSGGVAS
                     NSGLRAKITELAKDSGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            48570..51278
                     /gene="hpdB"
                     /locus_tag="PC399_001030"
     CDS             48570..51278
                     /gene="hpdB"
                     /locus_tag="PC399_001030"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF2591905.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKSAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATADGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            51282..51539
                     /gene="hpdC"
                     /locus_tag="PC399_001031"
     CDS             51282..51539
                     /gene="hpdC"
                     /locus_tag="PC399_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF2591906.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            51532..52482
                     /gene="hpdA"
                     /locus_tag="PC399_001032"
     CDS             51532..52482
                     /gene="hpdA"
                     /locus_tag="PC399_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF2591907.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLGELQDIFLDNGIACYVGHETAF"
     gene            52797..53759
                     /locus_tag="PC399_001033"
                     /pseudo
     CDS             52797..53759
                     /locus_tag="PC399_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(53858..>54444)
                     /locus_tag="PC399_001034"
     CDS             complement(53858..>54444)
                     /locus_tag="PC399_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2591908.1"
                     /translation="SNKKNLYPIKNIKLGSLLIIYFMISVIVPTSFILILQGSQPNYS
                     GIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFNKFGKKLGVIILGICWSLWHLPL
                     IFTLYTPETPILGLILRSIHIVGISIFLGYLYIKTKNIWFCAIIHILNNSAFLITNSK
                     ESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT        21932 a         5790 c        10319 g        16403 t
ORIGIN      
        1 ataagtttta atgatagttt atagtaagat attaaaataa atttatctaa tctagatatg
       61 aaataagtta ttactttaat ataaaaatga atctaatgga ataatattga tgataagtaa
      121 tatttagatt ttgtatttgt ttatagttaa taagtggatt atagaattta taataattaa
      181 tatatattaa acaaagatat attaagatat tttagaacat aaaactgaat taaggcgcga
      241 gtactatttt gtattcgcgc cttgatggtt tgagttattt gagtaaaaac taactttaat
      301 aagaaatatg tatttattaa gttgatattt ttaatgtgtt tagagattat ttaacaatct
      361 ccaaatataa aactagaatt atttacataa aagttaaata gtttgtatag agttagattt
      421 atatagtata atacttagtg taaataatta agaagttatc aaacaggagg ataaaactgt
      481 gttagatata aattcttttt taaatggatt ttttaatata atattaagaa tgagtatata
      541 tgatttaatt gacatatcta tagtagcgta tattttttac aaaatattta tgtttataaa
      601 agatacaaga gcagaacaag tgtttaaagg tataatattt cttttattag caactcaact
      661 tagtaacacc tttaaattgc ataccgttta ttggatatct cttaaagctt tagattatgg
      721 agttatagct gctcttataa tttttcaacc agaatttaga gcaggacttg aacatatagg
      781 aagagcaaag tttaatttat ttggaaagaa tgtaaatact tctgaagaaa ctctcaatag
      841 aaatatagaa gaaattgtag aagctttata ttcactttct agacaaaaaa taggagctct
      901 tatcataatg gagagagaaa ctagaataag tgatataata aatacaggaa ctattattga
      961 tgctgaaatt tctaggcaac tattaataaa tatatttata ccaaatacac cattacacga
     1021 tggagctgtt gtaattagag attctaaggt aaaagcagct gcatgttttt tgccattgac
     1081 agaaagtaag gatttaagta aagatttggg cactagacat agagcaggaa taggggtaag
     1141 tgaggtgtct gactgtataa ctttgattgt atcagaggaa acaggtggcg tatcaattgc
     1201 taaagcaggt aagttatata gagatatttc tagagaaaga atgatgaata tattacgtag
     1261 taatttaaaa acaaatacag aaacaagaag ttttttcaaa ggtggtatat ttaaatgata
     1321 aatagactaa aaaataatac caaaataaaa ataatatcat ttttaagtgc aattgcatta
     1381 tggatgtatg ttatggctgt agttgaccct gaagaaacta aagggtttga agatatacct
     1441 gtatctataa gtaatatgag tgatttaaag gataaaaatc ttgttatata tccagacgaa
     1501 aaattaacag cagatgtata tataaaaggt agtttatcaa aggtacaaag tgttaaaaaa
     1561 gaagatatac atgtatatgg aaccattgaa gacccaatac aaggacagaa ggaagtatat
     1621 ttaaaagcaa atattcctca aggtgttact ctagaattta agcatgatgt tttagtagta
     1681 aatcttgaaa aaaacataaa agaaaaaaga gatataaagg tagaagtaag tggaaattca
     1741 aaaagaaata taaataaaat tgaacttagt aaggaatttg tcgatgtatc aggtccaaga
     1801 actctagtta aagaagtcaa aagtgtaaga gctaatctag atgttggtaa tgagacagat
     1861 gatttttcta aaaaattgca attaatacca ttaaatagtg atggaactaa ggttgaagga
     1921 gtaacattaa gttcatcctt tgtgactgca aagatagagc tattaaagga aaaaattgtc
     1981 ccaataagag taagtctaaa tgataataaa gataatgatg tactgaaaaa ctataaatta
     2041 agtaaggaac aagttacaat aaaaggaaag aaagaagata tagacaaaat aagttatatt
     2101 aagacaaaac ctattgactt agctgatttg gctaatggtg aagaaaaaga agttagctta
     2161 gaaattccag agggtataac tcttgaggat agctctataa caattaaatt aaatgataca
     2221 aaacaattga gtgcagaatt tttatataat gcgggagata ttgaattaag aaatatccca
     2281 acaaatatgg ataatccaaa tgtaatttct gatgtagata taaaagttgt tgtagagtca
     2341 acggaggatt tgagtaagtt aaataaaaaa gatatttcat tatatattga tttatcacaa
     2401 ggtcctgagg aagatagtaa atataaaata aaatatgaaa caacatatca atttaaaaaa
     2461 ataaatatag aacctaatat tgttgaagtg gaatgaacaa atatttcgaa tatttagttg
     2521 ttacaagatg cgacaaaaaa acctataaaa ttaagtttgt taattttatg tttttgttgc
     2581 atcttgttta ttttagttat attataaaat aaggacaatg agatatttgt taaatctaaa
     2641 aagcgctaga tatatgaaaa aagactgata aatagattta aataaatatt ttgaattaaa
     2701 tatttcaatt atctctagga aaacgttgct gaacgagtta taatttaatt gagatataaa
     2761 aagttctatt tcaaaaaatt caagatgagg tggaaatgca tgagaagttt tgaagaagta
     2821 attaagtttg caaaagaaag aggacctaaa actatatcag tagcatgttg ccaagataaa
     2881 gaagttttaa tggcagttga aatggctaga aaagaaaaaa tagcaaatgc cattttagta
     2941 ggagatatag aaaagactaa agaaattgca aaaagcatag acatggatat cgaaaattat
     3001 gaactgatag atataaaaga tttagcagaa gcatctctaa aatctgttga attagtttca
     3061 caaggaaaag ccgacatggt aatgaaaggc ttagtagaca catcaataat actaaaagca
     3121 gttttaaata aagaagtagg tcttagaact ggaaatgtat taagtcacgt agcagtattt
     3181 gatgtagagg gatatgatag attatttttc gtaactgacg cagctatgaa cttagctcct
     3241 gatacaaata ctaaaaagca aatcatagaa aatgcttgca cagtagcaca ttcattagat
     3301 ataagtgaac caaaagttgc tgcaatatgc gcaaaagaaa aagtaaatcc aaaaatgaaa
     3361 gatacagttg aagctaaaga actagaagaa atgtatgaaa gaggagaaat caaaggttgt
     3421 atggttggtg gaccttttgc aattgataat gcagtatctt tagaagcagc taaacataaa
     3481 ggtataaatc atcctgtagc aggaagagct gatatattat tagccccaga tattgaaggt
     3541 ggtaacatat tatataaagc tttggtattc ttctcaaaat caaaaaatgc aggagttata
     3601 gttggggcta aagcaccaat aatattaact tctagagcag acagtgaaga aactaaacta
     3661 aactcaatag ctttaggcgt tttaatggca gcaaaggcat aattttaagg gggaaatagg
     3721 caaatgagca aaatatttaa aatcttaaca ataaatcctg gttcgacatc aactaaaata
     3781 gctgtatttg ataatgagga tttagtattt gaaaaaactt taagacattc ttcagaagaa
     3841 ataggaaaat atgagaaggt gtctgaccaa tttgaatttc gtaaacaagt aatagaagaa
     3901 gctctaaaag aaggtggagt aaaaacatct gaattagatg ctgtagtagg tagaggagga
     3961 cttcttaaac ctataaaagg tggtacttat tcagtaagtg ctgctatgat tgaagattta
     4021 aaagtgggag ttttaggaga acacgcttca aacctaggtg gaataatagc aaaacaaata
     4081 ggtgaagaag taaatgttcc ttcatacata gtagaccctg ttgttgtaga tgaattagaa
     4141 gatgttgcta gaatttctgg tatgcctgaa ataagtagag caagtgtagt acatgcttta
     4201 aatcaaaagg caatagcaag aagatatgct agagaaataa acaagaaata tgaagatata
     4261 aatcttatag ttgcacacat gggtggagga gtttctgttg gagctcataa aaatggtaaa
     4321 atagtagatg ttgcaaacgc attagatgga gaaggacctt tctctccaga aagaagtggt
     4381 ggactaccag taggtgcatt agtaaaaatg tgctttagtg gaaaatatac tcaagatgaa
     4441 attaaaaaga aaataaaagg taatggcgga ttagttgcat acttaaacac taatgatgct
     4501 agagaagttg aagaaagaat tgaagctggt gatgaaaaag ctaaattagt atatgaagct
     4561 atggcatatc aaatctctaa agaaatagga gctagtgctg cagttcttaa gggagatgta
     4621 aaagcaatat tattaactgg tggaatcgca tattcaaaaa tgtttacaga aatgattgca
     4681 gatagagtta aatttatagc agatgtaaaa gtttatccag gtgaagatga aatgattgca
     4741 ttagctcaag gtggacttag agttttaact ggtgaagaag aggctcaagt ttatgataac
     4801 taatgataaa agaataagaa tcataactgg tcactatgga agtggtaaaa gtgaatttgc
     4861 gatgaactat gttgttaagt taagagatat ggtttctggc aaagttgcta tagccgattt
     4921 agatgtggta aatgtatact ttagaactag agaaaagaaa gagttaatga aatccttagg
     4981 tatacaacca atagatagct caattaatgc tccaacatta gatttaccag cagtatcagc
     5041 agaggtaatg tctcctatgg tagaccattc atacaattct gttatagatt taggtggaga
     5101 taatgtaggt gctagggtta taggaagatt tagtcatttg ttaaaagaag gcgattatga
     5161 tatgcttttt gtaataaatg caaataggga gaaaactcaa acttctgaag aagttatcca
     5221 atatataaaa gaaatagaaa aatcttctaa attaaaggta acaggtctaa ttaataatac
     5281 tcatcttatt aggtttacta ctattgatga tgttttaaga ggtcagaaag ttgcaaaaga
     5341 agtttctgaa aaatgtaata taccgataag atatgttgca tgtttagaaa atcttgttga
     5401 acagttacca aaagatttag aaggagaaat ttttcctata aaattatata tgagagaaga
     5461 ctggatgtag tttgagctca aatatataga aagtttaagg aggcttaggt aaatggctaa
     5521 aggaaaagta tcttttaatc aagaacgttg taaaggttgt ggattgtgtg ttgaagcatg
     5581 tccagtaaaa atcatacaat tagactcaaa tgtaataaac aaaaaagggt ataacccagc
     5641 aactgttttt gaaatggaaa aatgcatagg atgtgcaagt tgtgcaacta tgtgtccaga
     5701 tgtagttata acagtagaaa gagattaatt taattaatat ttaaggggga attttaatgg
     5761 ctaagatact tatgaaaggt aacgaagcat ttggaaaagc agctatagaa gctggttgca
     5821 aatatttctt cggttaccca ataactccac aaagtgaatt accagaatat ttatcaagag
     5881 agttacctaa aataggtgga gcgttcgttc aagctgaatc agaagtttct gcgataaaca
     5941 tggtttatgg tggagcaggt gcaggagcaa gagttatgac ttcttcatct tcaccaggag
     6001 ttgctttaaa gcaagaaggt ataacatatg cagtaggagc agaagttcct tgtgttgtac
     6061 ttaatgtaat gagaggtggt ccaggacttg gaagtataca accttcacaa gctgactact
     6121 ttatgtctac aagaggtgga ggaaatggag actacagaac tccagtattc gcaccagcta
     6181 cagttcaaga agctgttgat atgataatgg aagcttttga cgttgcagat tattacagat
     6241 caccagttat ggtagttgca gatggtatga taggtcaaat gatggagcca gttgaattta
     6301 gagctcctga gaagaaaaga gaattgcctc ctaaagattg ggcaactgtt ggaactaaag
     6361 gaaaaagaaa acctaatgta ataaactctc tatacttaga gccagaagta ctagaagacc
     6421 attgttggca tttacaagaa aaatttgatg caatggaaaa gaatgaagta caatacgaaa
     6481 tgtacaaaac agaagatgct gaatttgtat ttgcagctta tggaactact tcaagagtag
     6541 taaaaagcgc aattgatata ttaagagaag aaggaataaa agcaggtctt ataagaccta
     6601 aagtattatg gccattccca ttcgaagcat tcaaccaaat tcctaatgct agaaacatat
     6661 tgactgttga aatgagtatg ggacaaatgg tggaagatgt taaaatggca gtagaaggta
     6721 aattaccagt ttacttccat ggaagaccag gaggaatgac tccaacacca gcagaaatag
     6781 ttgaaaaagc taaaaaaatt attgccggag aattggttgc aggaggtgct agataatggc
     6841 tgttgtattt aagaaaactg aaggattaca agatactcaa actcactact gtccaggatg
     6901 tacacatggt ataatccata gattagttgg tgaagtatta gaagaactag gagtattagg
     6961 tgatgctgta ggtgttgttc cagttggatg ttcagtttta ggatataaat attttaactg
     7021 tgacactcaa gaagctgctc atggtagagc gccagctgct gctacaggaa taaaaagagt
     7081 tcatcctgaa aatacagtat tcacttatca aggtgatgga gacttagctt caattggtac
     7141 tgctgaaata gttcatgcag ctgctagagg agaaaaaata actactatat ttgtaaacaa
     7201 tacaacttat ggtatgactg gtggacaaat ggctccaact acattagttg gacaaagagc
     7261 tactactgct caatcaggaa gaaatgctga aactcaaggt tatccaataa gagtaagtga
     7321 aatgttagct acattaacag gagctgtatt tgtagaaaga gtagcagttg atactccagc
     7381 tcatgttaga caagctaaaa aagctataaa gaaagcattc caagttcaac aagcaggatt
     7441 aggatttgga atagtagaag tattatctac ttgtcctaca aactggggac tagctccaaa
     7501 cgatgcttta caatggttaa gagacaacat gataccatac tatccattag gaaatttcaa
     7561 aaatgttgaa gttgaggagg tgaagtaaat gtctacagct agagtaatat gtgcaggctt
     7621 cggaggccaa ggtgttatgt ctatgggaca attacttact tatgcaggaa tgctagaagg
     7681 aaaagaagtt tcttggttac catcttatgg accagaaatg cgtggaggta cagcaaactg
     7741 tgcagtaact gtatctaatg agccagttgg ttctccactt ataacagatg atgcaactgc
     7801 agctatatta ttaaatatac cagcatttga aaaattcaaa gatgatgtta ttcctggtgg
     7861 aaaaataata gtaaatagtt ctttaataaa agaaaaagtt gatagaacag atgtagatgt
     7921 ttattatata ccagcaaatg aattagcagc tgaattagga aatgataaag ttgctaacat
     7981 gataatgtta ggtgcttact taaaagtatc tgatactgta gatatagaat ctgtactaga
     8041 agcattcaaa aaagttttcg gacctagaaa agaaaaattc gttccattaa acagagaagc
     8101 tttacaaaaa ggtatggatg ctacatgttc taaagcaact aactaataaa gaaaaagatg
     8161 tcaaaaaaaa gacaatagaa ttatttctat tgtctttttt gctgataagt attaaaatat
     8221 ttttatgact gaaattaagc ctataatgat attttatgaa ctataaaaat ataatacaaa
     8281 ttacaagctt ttcaattatg aaaggagaat ctttagtgag aaagtatttt ggaacagatg
     8341 gagttagagg agtagcaaat acagaactaa catgtgattt agcatataaa cttggtagag
     8401 ctggtggatt tgtcttagct caaggagacc acagagtaaa agtagtagta ggtaaggata
     8461 ctagaatatc aggtgatatg ttggaagcat ctttaatagc aggacttatg tctgttggat
     8521 gtgatgttat tactgttgga ataataccaa cacctgcagt ggcatattta actagaaaat
     8581 atggtgctga ttgtggtgtg gttatatctg catctcacaa tcctgttgaa tataatggaa
     8641 tcaaattctt taataagaat ggatataaat tagatgatga aattgaattg aaaatagaag
     8701 aatatataga cgatatagac aaaattgatt gtcttccaat tggtgaaaat gtaggaagaa
     8761 aattacatga acattgtgca caaagagact atgtagacta cctaaaatca ataataagca
     8821 ctgactttaa gggattaaaa gtagttttgg actgtgctaa tggtgcgtca tataaagtgg
     8881 caccaatagt gtttgatgaa ctaggagcaa gtgtaatttc aataaatagt tcaccagatg
     8941 gaaataatat aaactataaa tgtggctcta cacatccaga acaacttcaa agggctgttt
     9001 tggaacataa tgctgactta ggtcttgcat atgatggaga tgctgataga ttaattgcag
     9061 taaatgaaaa gggtcaaatt gttgatggag accatataat gatattgagt gccttaaatt
     9121 taaagaaaaa taataaattg gcacaagata ctttggtagt tacagttatg agtaatatag
     9181 ggttgacaat agctgctaag gaaaatggaa taaatttatc tactacagca gttggagaca
     9241 gatatgtttt agaagatatg gttaaaaatg gatataatct tggtggagag caatctggac
     9301 atatgatatt tttagattac aacactactg gagatggagt gcttagttct ctaatacttg
     9361 caaatataat cttgcaagaa aagaaacctt tatcagaaat tgcatcaata atgagccaat
     9421 acccacaagt attagtaaat gcaacaataa aaaatgaaaa taagaacaaa tatatggaat
     9481 atccagaaat aaaaactgag atagaaagaa tagaatctat tttagacgga aatggtagag
     9541 ttttgataag accatcagga actgagccat tagttagagt tatgttagag ggtaaagagg
     9601 aaggtcaaat aaaagaatta gcaacaaact tagctaatct tatacaagaa aaattatcgt
     9661 aatttaaagg gtattctcag atataaatac tgaggacacc tttctaagcg ccagaactta
     9721 gttatagaca taattattga gtaatatagg aactaagttg acgaggtcag agttaatcga
     9781 attgtcggcg gatgctctgc ggtgtgttta ccatcgaaag acttctttaa ataataggag
     9841 caatcctatg gacaaaggga agccaaagta aacttatatg acctaactct taaaatatat
     9901 aaataatatt tttaggagga cacaaataat gtgtggaata gttggatatt taggaagtag
     9961 aaaggcagca gaagttatag tagaagggct ttctaagcta gagtacagag gttatgactc
    10021 agcaggagta gcagttaata gttctaatga aaaagaactt aatattagaa agtttaaagg
    10081 tagactttct gtacttgcag aagacttaga aaagaatcct attgatggaa atttaggtat
    10141 agggcatacg agatgggcaa ctcatggaga accatctgat gtaaactcac atcctcattt
    10201 taatcaagcc aaaacaatag cagttgttca taatgggata atagaaaact atatggaaat
    10261 aaaagaagaa ttaatttctg aaggtgttaa gtttgaatct caaacagata cagaggtaat
    10321 agcacattta gtagataaat actatgaggg taatttatta gatgctgtat acaaaactat
    10381 atcaaaatta agaggagctt atgcattagg tgttatatgt aaagaacatg gaaatgaatt
    10441 agttgcagta agaaaagata gtcctcttgt agttggtgtt ggtgaaggtg aaaactttat
    10501 agcatcagat atacctgcat tattaaaata tactagagat gtttatttct tagaaaatgg
    10561 agaagtagtt catctaaaag acgaaaatgt aacagtttat gactctaata gaaatcttgt
    10621 tgaaaaagaa gtatttcatg taacttggga tgtagaagct gcgtctaaag gtggatatga
    10681 ctactttatg tcaaaagaaa ttcatgagca accaacagga gtaagagaaa ctttagaaag
    10741 aagacttgat gataatggaa atataattct agacagtata aatatttcta aagaagactt
    10801 agaaaaaata aataaagttt atatagtggc ttgtggaaca gcatataatg ctggattact
    10861 tggtaagtat gccatagaaa aatttgtaaa tataccagta ataactgata tagcatcaga
    10921 atttagatat agcgacccat ttgtagatga aaattcatta gtaatattag ttagtcaatc
    10981 tggtgagaca gcagatactc tagcagtatt aagagattca aaggctaaag gagcaagaat
    11041 actttcaata actaacgttg taggttcatc tatagcaaga gaatcagatg atgtattcta
    11101 tacttgggca ggacctgaag tagcagttgc atcaactaag gcatatacta ctcaaataac
    11161 ttctttatac atgatagctt tagactttgc tatcaaaaaa ggaacaataa ctagagaatt
    11221 ttatgatagt atgatttcta agatgaaaga aataccatca aaaatacaag aaatactaga
    11281 caatgaagaa tatataaaag aagttgctaa aacagttgtt agttctgaac atgcattcta
    11341 tttaggaaga ggtatagact atagcttagc aatggaaggt tcattgaaat taaaagagat
    11401 ttcatatata catgcagaag cttttgcagc aggagagcta aagcatggaa caatagcttt
    11461 aatagaaaaa ggaactcctg taattgccat agctactcaa gaaaagttat ttgaaaaaat
    11521 ggtttctaat atggaagaag taagagcaag aggagcttat gtagtagcaa tagctcaaag
    11581 ccataataag gatgttgaga aagctgctga taaaataatc tatataccaa attcagatga
    11641 tattttatca ccaatattag cagttgtacc aatgcaatta cttgcttatc atgtctctgt
    11701 actaagagga tgcgatgtag ataagccaag aaatctagct aaatcagtaa cagttgaata
    11761 aaaacagatt aaaaattaaa tagataatat ttattgaaaa gaactcatcc tgttatttgt
    11821 attttatagg atgagttttt taaataaata ttaaatttgt tttgtttttg ctataataaa
    11881 ttttataaaa agattgatat taaatcatct gtaaaatgat aaatatatag accataccag
    11941 gtatagggaa cgataaatat agttcatata aaatatagtg agtacttaat aaacatatag
    12001 tcaatatcag ataaaaaaaa taatagctta tgagaaaaat taaaatacaa ggtattagta
    12061 accttgtgat aagataaagt gagtagttta aaataagaat ataaggaact atttataaaa
    12121 gaatataatg aagctaataa agtataagaa aattacttta ttttagtaaa ataatttcaa
    12181 ataaaaattt tttaaattgg gagtataaat tatgacaaat gattttaaaa aagaaaagaa
    12241 agaagattat tttgttaatc ttaaggcaat atctactgaa gttttacaag aaaaagtgca
    12301 agataacgct tgggttatag ttgatactcg cttaaatgat gcttataatg gctggaagtt
    12361 agatggagta aaaagaggtg gacatataaa aggagcagta gatttttctg ctaattggtt
    12421 aagtgtatac tcagatagaa aagatgaagt gctagaacaa gcattgaaaa ctaaaagaat
    12481 tgatttagat aaaaatatag tattatatga tgcaaatgga aaagatgctt tggttgtagc
    12541 agattattta tctaaaaaag gatataaata tctatataaa tatgatatta aacaatgggc
    12601 agacaacgaa aatcttccaa tggaaagata taaaaattat caaatgatag ttcctgcttt
    12661 tataataaaa gatatattag atggaaaaat tcctgagaca tttgaagatt caaagaatat
    12721 aaaaatgatt gaagcgagtt ggggagaaga atcgtataca aaagggcata tacctacaag
    12781 tgttcatgta aataccgata ttatagaacc tcctccaaca tggatgcttg ataatgatga
    12841 taacttaatt aagtttgctt tagactatgg attaacaaag gatgatactg taatagtttc
    12901 aagtagtaca cctatggcat cttatagact agctgttata ttgagatata taggagtaaa
    12961 agatgtaaga gttttgaatg gtggaacaaa ctcatggtta tcagcaggat atgaattaga
    13021 atttactagt aatccaaaac attcatgtac taattttgga gcagatatac cagttaactc
    13081 acaattagtt gttactacct ctgaattaag acaaaaacta aaagaaaaaa ataagtttat
    13141 actagtagat aaccgtactt gggatgaaca tataggaaaa gtatctggat atacatatta
    13201 tgataaaaaa gggcgtatac caggagcttt atatggacat tcaggtagtg atagtgtttc
    13261 actagaagaa tatcgtaata ttgataatac aatgagaaat aaatatgaga ttttagaaat
    13321 gtgggacaag gaaaatatag atgtgaatag acagcttata tttatgtgtg gaagtggatg
    13381 gcgtgctgct gaagtattaa catacgcaaa tgtcatagga gtggaaaata cctcccttta
    13441 tagtgatgga tggatgggct ggagtcttga taattcaaat ttaattgaag tgggagaaca
    13501 caaataaagt ttttgaaagt acgatagagt tgtttaaaac aaaggtgttt ttgatttatt
    13561 gggaagagtg tgtaaattgt gttttgggaa gtgtacttag gtatgttcat ttggagctat
    13621 ggaaattgat aaaaaagtat tatctgttta ttttgctggt aaaatgtgaa tagattatag
    13681 attatagatt atagattata gatttaagta tagactaagt aatttatatt acagaagaga
    13741 atatataaga actagtgcaa gatgtatttg atacatataa aaaattggca aatccagaat
    13801 aaggatagag aaaattgaga ttgaagagtt tgaaagagac ttattagatg aaaaatacaa
    13861 agatgtataa aaatacatat tgagaaagta tatctctctt aaatttgtta ataattctat
    13921 tatacagaaa aacatattta agggggattt tttactatga aaagagtaaa attagctata
    13981 agctttttta tattaatgat tatagggatg acaatatctt atgcagatac aagtataaat
    14041 actaaataca atagattaag taaagctctt gaaatttcag aagcaaattt taaattttat
    14101 aatgttaaaa ttaattctgt tataaatcat aatttatcaa ataaagaatt tcataatatt
    14161 tttatagaaa taattaacaa tctaaagttg gataaaaaag gaataaattg gaatcaaaaa
    14221 gggtctggcg ataaaataca attatgtgct actataaaag gtagcgataa agaaatttct
    14281 ataaaaggaa taaataaaaa taacaaagaa agttacatag tagttgacat attggataac
    14341 aaagtatata aacataaaga ggttatttat agtaacgtag aaaatgtgtt aaataaatat
    14401 tcatctcaag tagatgtgtc tgcttgtatt gttggagaat acacaaaaag gttacaaaac
    14461 aacaaatatg atgatatttt acaaaaaata ttatataata tgagtgctaa gcaaatagat
    14521 gaagtgaaag acgtaaactt tttatctata acagcatata gtaaagtatt aaatgattat
    14581 gaattagaat atttagggga aaaaattaat ttaaatatag gaattaaata taatgaggac
    14641 gacggaagaa cactcatata tattgccaca ccaattataa aattagatta ttaattatga
    14701 ggagatcaaa aaatatggct aagataatag taaaaaagag taatccactt aaaggtagtg
    14761 ttaaaataga tggtgcaaaa aatgcagttt taccaataat agcagcgact ttattagcaa
    14821 acgggaaatc aacattaaat ggagtaccaa atttaagaga tgttcatgtt atatcagatt
    14881 tattaagaca tgtaggggca gaagttgaat ataaagaaaa tacacttact gttgatgcaa
    14941 gtaatataaa aacttgtgaa gctccatatg agcttgtaag aaagatgaga gcttcttttt
    15001 tggttatggg gccattgctt gctagattta atagtacgaa aatttctatg ccaggaggat
    15061 gcgctatagg aacaagacct atagatttac atttaaaagg atttaaagcc ttaggtgcaa
    15121 agatagaaat ggaccatggt tttgttgaag cagcaacaga aaaattagta ggaaataaat
    15181 tatatttaga tttcccatct gttggagcaa ctgaaaacat aatgatggca gcttctttag
    15241 cagaagggac tacaataata gagaatgcag cagaagagcc agaaatagta gatttagcaa
    15301 acttcttgaa cgaaatggga gcagatgtta aaggtgcagg tactaatact ataaaaataa
    15361 aaggtgttaa agagttaaaa ggagcagaac ataatgtaat tccagacaga atagaagcag
    15421 caacttatat ggttgcagca gctatgacaa aaggtgatat aactgttgaa aatgttttaa
    15481 tggagcattt aaaaccagtt gtagctaaat taagagaagc tggttgtgaa ataacagaaa
    15541 tggacaattc tgtaagagtt gtaggtccaa aagtattaaa accaatagat ataaaaactt
    15601 taccacatcc aggatttcct acagatgttc aagcacaatt tatggctatg cttactgtag
    15661 ctaatggaac tggtgtagtt atagaaactg tttttgaaaa cagatttatg catgttgctg
    15721 aatttaatag aatgggtgct aatataaaaa tagatggaag aagtgcggtt gttaatggtg
    15781 tagatgagct acatggtgca gcagtaaacg ctactgattt aagagcaggg gcagccctta
    15841 ttttatgcgg tcttatagca gaaggagaaa ctcaaatagg agaaatatac catatccaaa
    15901 gaggctatgt tgatatagat aagaagataa cagcattagg tggtcaaatc gaaatagtag
    15961 aagattaata attggtttat agaagaaagt tataaattaa tagggttgtt catatactta
    16021 aatacaaggt tttaaaagct aaggaggaga gtatatgaag aacccattgg ttgttttgtt
    16081 aggatttgtg acttgctctg tgttagttcc atcacttata actttagttt cttataaaaa
    16141 tgtagaatta actgaaaaag cagagagccc agtatctata aacaaaacta ttaaaaaaag
    16201 tgatattgaa gacaaaggta ataaagaaga aaaaaataca gtgaactatg aaactgtaaa
    16261 taaaaaagca ccaattatca atgtatataa tcatataaca gggaaaaccg aaaagatgga
    16321 catggagaac tatttatgtg gagtactagc tggtgagatg tcttcagagt ttgatataga
    16381 agccttaaaa gctcaatctg tagcagctag aacatatgta gtatataaac aggaacatgg
    16441 taaatctagt aaacataaaa atgcagtagt atgtactgat tataaacatt gtcaagaata
    16501 taagagttat gatactctta aaaaattaaa tggtgaagaa tggattaaga ataaatattc
    16561 caaaatccaa gaagcagtta gaggaactaa aggtcaaata ataacttata acgataaggc
    16621 gatacttcct ctttattttt ctacatcttc aggaaaaaca gaaaatagtg aagaagtatt
    16681 ttctgcaaaa tatccttatt taaagtctgt ggaaagtcct tatgataagt attcccctaa
    16741 atttgcgtca acacttaaaa taagcaacac agattttgtt aaaagtctta gaagggcata
    16801 tagcactatt gtgatagatg tgaataattt aagtaaacaa gtttcaataa ctaaaagaag
    16861 tgatgccgga actgtagaaa aaataaaatt aggaaataaa gaattgactg gaaaagacat
    16921 tagaactgtt tttaaattga actctgcaaa ctttgacata aaatttggag aaggatacat
    16981 agatttcgtg gtaaaaggat atggtcatgg tgttggtatg agccagtggg gagcagaagg
    17041 aatggcagaa gagggctata aatattatga tatattatca cattactata cagatacaaa
    17101 aataaaagat atatactaat aattgtataa aatgccaaaa acacgtcaat aatccaaatg
    17161 tagaaatata tttggaggtg caaaatgaag aaaaagctgt tagaaaaaga tggtttttat
    17221 ttatctttgt ttgtatgtgt ttgtttatta gcagtaggtg gagtttggtt tacaaataat
    17281 aatgtagata aattggcttc taataaagga attatggaaa atgctaataa agatagtgaa
    17341 gaggaaatac atttaattga aaaagacaag aaagatgcta tccctactgc aacggattca
    17401 aaacaaaact tagaaaaagc taagtcaaaa gaggaaaata aatcgagtac aacaaaatta
    17461 aattacattg gagataaagt aataagagga tattcagaaa aagaacctag ttattctaaa
    17521 acacttgatg tttgggaaac tcataaaggt gtagatatta gttgtactaa aggcaaagaa
    17581 gtaaaatctc tattaaacgg aatagtagta gatgtattta atgatgaaga atatgggcaa
    17641 tcagtcaaaa taaaaagtga caataacatt gtagtagtat attcaaatct agataaaaat
    17701 gttagtgtta agaaagaaca gaaggtaaca gaaggacaat ctctaggtac tgtaggtagt
    17761 acttctcaaa tagaaagtga agaaggtata catgttcatt tagaggcgta tagtggagaa
    17821 aaatctatag acccaatgag tctaattaag taaaagtata acaagtttaa tcatatttgg
    17881 ttaagcttgt tatatttttt ggtgttttta catatatatc attaaagcta ttatgtgagg
    17941 ggggtaatct ttgagatctc atatagagga aagggccata gtggtagcaa aatatatact
    18001 tgaaaaaaac actacagtaa gacaaaccgc caagacattt ggagtaagta aaagtacaat
    18061 tcataaggat gttacagaaa gattgaagga aataaatcca tcccttgcca aagaagttaa
    18121 aaatgtgctg gacaaaaata aatcagagag acatattaga gggggattag caacgaaatt
    18181 aaaatatgag aaggaacata aaaaaatgta ggttaaaaac ctactttttt tatttaatta
    18241 aattataaaa agaaatatat taagtaatta taaatagcaa aataataata ctctcaaaag
    18301 atttattttc tcttgattaa agcgagattt ttgtatatgc tttattatag gagtattaaa
    18361 ataggcttat aatataatct aatattataa ataaaggagt tgaaaaaatg gctggagctg
    18421 atataggcat agatttaggt actgcaaatg tgcttgttta tgttagtggt aagggaatcg
    18481 ttttagaaga accgtctgtt gtggcgatag acaacagaac agattctgtt ttagctgtgg
    18541 gagaagaagc taaaaagatg atagggagaa caccaggtaa tatagtcgct ataagacctt
    18601 taagagatgg tgtaatttca gattatgatg taactgagaa gatgttaaaa tcttttatag
    18661 ataagatagt tgataaaaag ggatttggaa gattttttat gccaagaatt atggtatgcg
    18721 taccaacagg tgtcacagaa gtggaaaaga gagcagtaga agatgctacg agacaagctg
    18781 gagctagaga agtatatata atagaagaac caatagcagc agccatagga gctggtgtag
    18841 acatatcaca gcctaatggt aatatgatta tagatattgg tggaggtact gtagatatag
    18901 ctgtaatatc tctaggagga gctgttgtaa gtgaatctat aaaggttggt ggagatagat
    18961 ttgatgatgc tatagttaaa tatatgaaaa aacaacacaa tcttcttata ggagaaagaa
    19021 cagcagagaa aatcaagttt gaaataggtt ctgcatttaa gagagaagaa gaaaaatata
    19081 tgaagataac aggaagaaat ttaattacag gacttcctag ttctataact ataaattcaa
    19141 cagaaatgtt ggaagcactt agagaatgtg ttgagcaaat agtagttgct actcatgctg
    19201 ttttggaaaa aacgcctcct gagttagcag cagatatagg agatgttggt ataataatga
    19261 ctggaggagg ttcattatta tatggattag ataaaataat agaacagaga acaggaataa
    19321 ctgttacaat agcagatgaa ccattatctt gtgttgcaaa aggtacagga atagctttag
    19381 gttcaataga cctacttgaa actggcgggt ctttcaaaag aaaataatta ggagagttta
    19441 gaatgttaaa tatagatgaa ataaaaaaat taataccaca tagatatcct tttttattag
    19501 tggacaagat tacagagcta gaagttggga aaagagctgt tggtataaag aatgtaacag
    19561 ttaatgaacc atttttccaa ggtcactttc cagaatatcc tttaatgcca ggagtattaa
    19621 tagtagaagc tttagcacaa gtgtgtggag tggcaatgat gtctgtagag gaaaacaaag
    19681 gaaaactagg tgtatttgca ggtatagata aagttagaat caagagagaa gtaagaccag
    19741 gagatacatt aactatggaa gttgaaatga caactcttag aagaaatata gccaaggcag
    19801 atgctaaggc atatgttggt gaagaacttg tctgtaaagg ggaattaatg tttgccttag
    19861 tagaaaaata aaaacttgaa atataaaagt aaaaaggtag ataatttgga gtatttaatc
    19921 agatactcta aaatatctac ctttttatat aaagaatttt atcatttagt ttatatttag
    19981 attaggtgga tactaataaa gccaaggcta ttgtttcagc taagtctaat ataaagctaa
    20041 gacgtatatt attaaatgaa agtttattat tttcatcaat tttatctaca attcctacta
    20101 tagaataatt tcctatttga ggtagttttt tgccaacacc ttttccgggc aggataggtc
    20161 cttctctaat ctgtatatta cctatgttac tctgcgaccc caagcaagca tctatagcca
    20221 agaagttacc ttgtatatgt gtatttttta tagtatctaa agactcatat atatttaagg
    20281 cgtgaatagg gttatctagt gtaccgtaaa cggggtattt aaagttactg ttcttaagta
    20341 ttgttccaac taaagggcct aaagtatcac ctatagccct gtctgttcct atgcaaacaa
    20401 caatcgtatt ttcattaatt atagttttta gaactgaact tagcatctgt ataacatcat
    20461 catcgctgta atttacttta gctaaataca ataggaacac ccccttcaaa aaaatatatg
    20521 aataaaaagc attaaaaaga cctaacttta aatttcagat agatagaaaa ttttatttgt
    20581 cttacaaaaa aaggaataga aaaaatatga ctaaaaatat tgacaaaatt ttaaaaaaat
    20641 agtaagctag taaagtaaaa caaaacttaa atcttgatta ccttatcaag agaggctgag
    20701 ggataggccc tgtgaagccc agcaaccacc caaatgggaa aaggtgctaa ttcctacaag
    20761 acaaaagtct tgaaagatga ggtcagttaa ttagtatgta attacgatta ttaacctctt
    20821 cttataaagg agaggttttt tattttgagt aaatttctaa aattaaaaat aaggtaaaac
    20881 ctataataaa aactaagaat aaataagggg gaataaaaat ggcaagacat ttatttacgt
    20941 cagaatcagt tacagaagga catcctgata agatatgtga ccaaatatca gattcaatat
    21001 tagatgcatt attagaaaaa gacccacaat ctagagtagc ttgtgaaact acagttacta
    21061 ctggtttagt tttagtagca ggagaaataa gtacatctgc ttatgtagac attcctaaat
    21121 tagtaagaga aacagtaaga gaaattggat atacaagagc aaaatatgga tttgattgtg
    21181 atacttgcgc agttataact tctattgatg aacaatctgg agatatagct atgggagttg
    21241 atgaaggcct agaaagtaag actggagaag agatagaaga agaaatagaa aaagttggag
    21301 ctggagacca aggtataatg tttggatttg catgtaatga gactccagaa ttaatgcctc
    21361 ttccaatatc tttggcacac aaattatcaa gaagacttac agaggttaga aagactggtc
    21421 tagttgatta tttaagacca gatggaaaaa ctcaagttac tgttgaatat gaaggaagta
    21481 aagctgtaag agtacataca gttcttatat cagctcaaca ttgtgaaaca gtatcaaatg
    21541 ataaaataag agaagattta attaatcatg ttattaaaga agttatacca gcagaattac
    21601 ttgatgaaga tactaaaatt tatataaatc caacaggaag attcgttata ggaggaccac
    21661 aaggagatac aggtcttaca ggaagaaaaa taataataga tacttatggt ggatattcta
    21721 gacatggtgg aggagctttc tcaggaaaag accctacaaa ggttgataga tctgctgctt
    21781 atgcagctag atatgttgca aaaaatattg ttgcggctgg tcttgcagac aaatgtgaga
    21841 tagaacttgc atatgctata ggtatagcta gaccattatc tatatttata gatacttttg
    21901 ggacaggcaa agtttcagaa gaaaaacttg ttgaattagt aaataaacac tttgatttaa
    21961 gaccaggagc cataataaga gacttagatt taagaaagcc tctttataaa aaggttgctg
    22021 cttatggtca ctttggtaga actgatatag atttaccttg ggaaagaact gacaaggttg
    22081 agcagttaag aaaagatgct ttaggtgaat aatttttaac taaaaagctg ttagaatgta
    22141 attttacgat tactttctaa cagctttttt attatgagtt aaatattttt aataaatgtt
    22201 aatttatcat catataatga ataaacagta tttaatctgg tcaatacatt ttatataata
    22261 ctatactaaa tttatgcata ttatgggata tatgttaaag gatggttaag aatgtgttaa
    22321 ctaatgttaa ataattaaaa atatattgaa atatcataat aatgagtcta atataatctt
    22381 aataaactta cactttaaaa ataattttaa ttggggggag tagtgtatgt caggcaaagt
    22441 aaatattaaa acggttataa gttttgctgg agcttatgtt gctactgtaa taggttctgg
    22501 atttgcaact ggtcaagaaa tactgcaatt ttttacattt tatggatatg ctggtattat
    22561 aggtggaata atctctatgg tactattttc atggtttgga gcagaggtta tagataaggg
    22621 aagagaattg aaacttaagg agccaataaa aatttatcaa gtatattgtg gaaaatatct
    22681 tggaacattt tttgaatggt ttggtccatt gtttttattt ggaacattag taataatgat
    22741 tgcaggagca ggagctacat tatcagagta ctatggatta aatccatatg taggtaggat
    22801 tggtatgaca atcgtttccc taataactgt ttcattaggt ttgacaaggc tttctaaaat
    22861 actaggtaat ataggaccta taataataat atttacactt ttagtaggag caattagttt
    22921 atttagcaat attgatgctt tatcaaatgc tggaaatatg gttgatagtt taaatattaa
    22981 aactgctaca tcaaatggat atttctcagg agtactttat acttgttata atgtaataat
    23041 agtaataaca ttcttgacag gaatgggagc ttctgcagta aataagaaag atgctgtgtg
    23101 gggtggtata gtaggtggag tagcacttat ggcagcagct ataatgatga atttggcatt
    23161 actttcagac ataggaaata tatacacaaa agagatacca gcactttact tagctgataa
    23221 gatatctcca ataataggta ttttattctc agtagtactt ttactaggaa tatacactac
    23281 agctgtacca ttgttatggt cagtaactaa ccgatttgta gaagatgacc accctaaatt
    23341 taaaattata acaatagtag taagtatatt agcttgtata ggaggtctac tgccatttga
    23401 taagttagta ggtacactat atccatatac aggttacatg ggaattttaa ttttactatg
    23461 tatgctttat agaagaatta ctaaaactga ggggtacaag gagaataaat ctgaaataag
    23521 ctagtactgt aatatactta tgaaatttaa tttatactct aaatatggag taattaaaat
    23581 aaaatagcta tttaaatatg gtacctgtat ttcatggtac catattttat ggatgcagat
    23641 tttttttgtt tctgataaaa tgtataatag ttggaaatta cgttaagtat aaaaatataa
    23701 aacttttcta cgattatcat agttttaata tataaaatta tactaacatt taaatggagc
    23761 gattattagt atttttagga gataaaaatg gaaaaattag aaggaatgat aagtgaaata
    23821 gtctttaaaa acgaagataa tggttataca atagctcatc tggtaaatga aaatgatgaa
    23881 attgtcgttg taggatgtat gcctactttg gcaattggag aaagcataga agttgaagga
    23941 aagtgggtaa accataagat ttatgggact caatttgaag tgaatagttt tatgccagta
    24001 acaccatcat ccttagaagg aatttatgtt tacttatctt ctggaatgat acatggtatt
    24061 ggtgaaaaaa tggctaaaag aataattgat aagtttggtg tagatacgtt agaggtgata
    24121 caaaattcac cagaaaaact acaagaagta gaaggtatag ggagtaaaaa agtaaaacaa
    24181 atagtaaaaa gttatgaaga agacagagaa ttgagaaata taataataca gctatctcct
    24241 tttggaataa caccaaatta ctgcctaaaa atatataaaa aatataaaag cagtgcaata
    24301 gaagttataa ataaaaaccc ataccaactt gctgaagata taagaggaat tggatttaaa
    24361 gtagctgata gtatagcaaa taaaattggt atagataaaa attctaagga tagaatttgt
    24421 cagggaatct tatacacttt aaataaatct ttaagtaatg ggcatacata tttaccagag
    24481 catgttctaa tacaagattc ggaaaaatta ttggagctaa atggagaaat aataaaagag
    24541 tgtgtaatga tgctagttta taatcaaaaa atacatgtag agaaagtaaa caatgaaaat
    24601 cttatatacc ttatgccata ctatttagct gaaaatggag tatgtagtca aatagtaaaa
    24661 ttatcacagt atgaatttga agacttaaaa atagatatag ataacgagat taatgtttta
    24721 gaagaagata agaaaattaa gttagcagaa aaacaaattt tagcagtcaa agaatctgtt
    24781 aatagtggcg tattaattat aaccggtggt ccaggaactg gtaaaacaac taccattaat
    24841 gctatcatag atatatttga aaataatggt aaaagcgtga ctttggcagc tccaacggga
    24901 agagcagcaa agagaatgag tgaaacttct aataaagaag caaaaactat acataggttg
    24961 ctagaaatgg gattttcaac agatgatgat ttaacttttt ttaaggatga agaagaccca
    25021 ataaattcag atgtaataat tgttgatgag gtatcaatgg tagacattat cttaatgtac
    25081 aatttactta gagctataaa gttaggaaca agagtaattt tagttggaga tagtgaccaa
    25141 ttaccttctg taggtgctgg aaatgtactt aaggatatga ttaattctaa tattataaat
    25201 gtagttaaat taaatgaaat atttagacaa gcgcaagaaa gtatgataat tgtaaatgcc
    25261 cataagataa acaatggaga accactttat ctaaatacaa aaggtaaaga tttctttttt
    25321 ataagaaaat caactaatga agaaatttta aatgaaataa taggattggt taatgaaaga
    25381 ttacctaaat tttataaagt tgataaatta aaagatatac aagttttatc ctcaatgaga
    25441 aaaggtgaat tgggagttac taatctcaat atagagttac agaagtatct taacaaaaaa
    25501 gaaaaattta aagttgaaga aagtttttct aaaagattat ttagagttgg cgataaggta
    25561 atgcaagtaa aaaataatta tacaaaaaaa tgggaaacag aagaccaaaa agaaagtgga
    25621 gaaggtatct ataatggaga tataggatat gtttatcaca tagataaaga taaaaaaact
    25681 atttatatat tatttgacca gactaaaatt gtatcatacc tatatgatga attagatgaa
    25741 attgaccata gtttttgtac aacaatacat aaaagccaag gaagtgaatt tccagttgta
    25801 gtattgccaa tagcttgggc tcctcctatg ttacttagcc gtaatttatt atatacagca
    25861 gtaacaagag caaaaaaatt agttgtatta gttggagatg tgaaatatct tgaatatatg
    25921 ataaaaaata atagagttaa tcaaaggtat tcaaatttag gatataagct aaataaattt
    25981 aagcaagaag ggctgttgat agaataaata tgaaaatatg caatgataat tttattaaga
    26041 attttaaaaa tatgattaat aaatgtttgg attttatata tcctgagaat ataagttgca
    26101 taatttgtga taaatccata aagaaaacaa atacttattc aatatgtaaa agttgtttta
    26161 aagaaatgaa ttttatacaa gatggttgca tgaagtgtgg aaaaccaata atacgtcatt
    26221 caattgaaaa agaatttatt gaagaatgta gttattgttt taataaagac ttttattttg
    26281 ataaatcaat atcttgtatt gaatacaatg atgtgacaaa aaagatagtg ctagggctga
    26341 agtataacca aaaaaccttt atggctaagt atatagctca aataatgaaa gaaaaattat
    26401 atttagaaaa cataaaattt gattatatat tatttgttcc acttcataaa aaaaggctaa
    26461 ataaaagagg ttttaatcag gcacaaaaaa tagcatttaa tttaagtaaa atagtaaata
    26521 ttccattatt ggattgtata agtagaaaaa aatatacaag aatgctatat aaattgaata
    26581 agaaagaacg aaaagaagaa ttaaaaaatg tatttgttgt taaagacaat gttaaattaa
    26641 taaataataa aaatatactt ttaatagatg atatatttac tactggtcta acaacaaacg
    26701 aaatttctaa acttttaaag ttatcgggtg ctaataaggt ttttgtattg actctactta
    26761 caaaagcaaa tgataattat attatggaat aatcttatat attgacaatt ttaatttaaa
    26821 atattaaaat gaataagtga tatttaggtc tgtggttgaa agtccaagcc agtcgcaggc
    26881 aaagggatcc acgtaagtta acactaaaaa ttgggttaat gatcatggtg cggcttagaa
    26941 gtaagaccta ccgataataa aatcgagaga gagtagtagt gcggcgaacc caagcgaacc
    27001 ctccagtagg cgagtgtggg gtcaaaaacc aggtcagcta aatatcgtat taaaagggag
    27061 ctagcatacc tttatgtata gctctttttt atatataata aatttaggtt gagtcataaa
    27121 gaggtgatat gtttgaataa aagacaggaa aacttattaa caacgttgct attaaaagaa
    27181 gagtttataa atgttaataa gattgccaat gattttgagt gttcagaaag gacaataaga
    27241 aatgattgca aatatattga tgaatggtta tctacttttt gtaatgctta tatcaaaaga
    27301 aaacctaata ttggaattaa atttagtgga gatggaacag acaaaatact ggtaaatcag
    27361 aagttgcgag gtgaagttaa aagagtaaat agtgagcttc ataagcaagt tgagatatta
    27421 aatttactac tttgtaataa taaaaaagtt actttaaatg atttatctaa taagttgtac
    27481 ataaataaaa atattgtcag agaagaaata agtaaattat cagtggcatt aaataattat
    27541 aatttaaatg tctcgactaa aaaaggcagt ggtatatata ttggagggga tgagaaagac
    27601 ataagaataa tgttagtatc attcttattt aaatacatag ataaatacag attaaacata
    27661 gatgaaatag agtacttcca tatagtagat ataataattg taaaaaacat aatgtttact
    27721 atagaggagg atatggatat aagacttaca gatatatcat tcaagcaatt gatgctattt
    27781 ttacttataa atataatacg aataaggcta ggaaattctt taaaagtatc aaatactaag
    27841 aaagagctta agctaaaaat gctgatatta gatatagaag atgaattgaa atctaattta
    27901 tccatagtat taaattatga agaaaaattg tttatagagt ctttaatatt gggtatgaac
    27961 aaacaaataa atagtagtaa tattaagaaa tctttatatc taaatgaaga attagcaagt
    28021 tatacaaaaa aaattataaa tttagtatca gaagaatccg gaataaattt taataatgat
    28081 atattacttt atgaacaatt aatatgtcat ttgaatgtta caatgcacca aatgaaaagc
    28141 aatgtttact tagaaaatcc acttttagat aacataaaaa ctaaattttg ttttttattt
    28201 acagtaatat caagttcaat agaaattgaa ttaaaaggaa aacaaataac agaagatgaa
    28261 attggttatc ttacacttca ctttcaaacc tctctagaaa gaaaatacag caaaagggaa
    28321 tcaaataaaa aggcaagtat tgtatgtcca tttagttttg gagtaagcat gctattaaaa
    28381 gtaaagatag aaaagagatt tgataacata aaaattattg aaacattaag agaggaagat
    28441 ttaaaaagag ataacttcaa taagactata gattttataa ttgcatttca agagtatgaa
    28501 aatatccaaa aaccaatttt tataacaaca ccattattta ctgatgagga tgagaataaa
    28561 cttaaagagt tcacaaacaa aataaaagaa aaagacactt catataaact tatgaacaga
    28621 ttaatgatgt ctgattattt aataagagag ttagaacatg atgatattta tgaagctata
    28681 acatatttaa ctaatttatt aattgaaaaa cattatgcag aaaaagagta tttacaatct
    28741 attataacaa gagaaaagtc atatccaacc aatgttggaa aaggaatatt attccctcat
    28801 ggagatatga aatatataaa acaatctgtt ttatgttttg caaggttaaa aacaccaata
    28861 agaattagaa atggaaagga tataaagatt atacttttgt tagcatataa aaaagatgat
    28921 gatagaaaag tatttagaga attatttaaa gaaatatcta atttaacaga ggatgaaaat
    28981 atgctagata ttctaagtaa atgtgacatt gaaaaagtaa aagatattct tatataaaat
    29041 tcatagtgaa ctatggataa gcacaaaatt ttccatgtga tggaaaaagt tgtgcatgtt
    29101 tttttgtttt tataagatat tatatgtttg taaggtataa ataatctaaa agtagagaag
    29161 caacttagga ggaaaaaatg gaagaaatag ttttaaagat tataattcat gctggaaatg
    29221 caaaatctat gctttatgaa gcacttgact atgcaaagga aaacgatttt aaaaaagcag
    29281 atgaattaat tgaaaatgca aatgaagaaa ttttaaaagc acataaagta caaactgaac
    29341 ttatacaaaa ggaagctggg ggagataaat cagatatatc tattttattg attcactctc
    29401 aagaccattt gatgacttgt atgagtgaga gaaaccttat aaaagaaatg attatgttaa
    29461 gaaaagaaat tcaaaaatta aaataaaaaa attaaggggg atttaaaaaa tgaaaagaaa
    29521 agtatattta ttttgtagct ttggtatgtc aacaagttta cttgcagata aaatgcagaa
    29581 ggtagcagat gaacacaatt taccaataga agtagaggca tttccgatag cagaaattga
    29641 caaaatagtt gaagaaaaaa atccagattg tatattacta ggaccacaag ttaaatacat
    29701 gctaaaggaa ttaaaaccaa agtttgaagc tcaaggaaaa cttatagata taatcaatga
    29761 agttgattat ggaactatga atggggaaaa agtattaaaa ctcgctataa agctaataaa
    29821 atccaaacaa gcgtagaatt atagtaaaaa ataataggaa atgagggtag atagatgaat
    29881 agattggaaa gaatacttat gccattggct gaaaaaatag gtaaaaataa attattaatt
    29941 gcaattagag atggtttttt aatatcatca ccattattaa taataggttc attattttta
    30001 ttagtagcaa attttcctat aaaaggatgg aatgaatttg taggtcaaat ttttggttca
    30061 gattgggctg taaaattgca acaaccaact gtagcaacat ttgaagtaat gactctactt
    30121 gcagttttag gaattggata ttcttatgca aagcaaatag atgtagaccc aatagcatca
    30181 gcagcagttg caatagttgc cttttttata ataacaccat ttgtaatacc atatacacca
    30241 gaaggaacag aatcagtcta catggtaaca ggtataccac taggttggat gggctctaag
    30301 ggaatgtttg taggtatgat aacagcaata acctctgtaa aattatttgc tgcagttgta
    30361 aaaaaaggat ggacattgaa aatgccagat ggagttcctc caaccgtatc aaaatcattt
    30421 gcagcattaa taccaagtgc aatagttatg gtagttttct ttttagttaa gatagctttt
    30481 gaagctacac catatggaag tatacatgaa tttatattta agtttttaca aatgccatta
    30541 ttaaaattag gaaatagttt aggtgctatg gcaatcgctt atatattctt acatttattc
    30601 tggttctttg gaataaatgg aagtagtgtt gttggggctg tgtataatcc aatattaaaa
    30661 attctatcag ctgaaaattt agctgctttt caagctggtt caaaaatacc aaacataata
    30721 acaggtcaat tccaagatat gtttgcgaca tttggtggag caggtagtac actatcatta
    30781 gtcatagcta tgttattaat atgtaaaagt aaacgtataa agtcaatggg aaaactatca
    30841 attttaccag gaatatttgg tataaatgag ccattgatat ttggtctacc aataatgctt
    30901 aacccaatac tattgatacc atttgcaata gttccaacaa taaatataat aatagcttat
    30961 ttctgtatgt cagcaggatt agtgcctctt acaaatggag ttcagttacc gtggacaact
    31021 ccaattatat tctctggttt tttaacaaca ggatggcaag gtgcagtctt acaagtagtg
    31081 cttcttatac taggaatatt tatgtatata ccatttataa aaatgctaga taagcaatac
    31141 ttaagagaag aaaatgcaac tgttgaagag gaagatgatg atatatcttt tgatgattta
    31201 gtattatagg aggaagatga tgaagaaaat agttatgata ttggatcaaa tacaagctgg
    31261 agctggagga aaagagaaaa gtaatatacc tcctgctgga aaatcaagcc cattaggacc
    31321 aggtataatg atggagcaat ttttaaatga atctaaggta atagctacct tattttgtgg
    31381 ggatgaattt tttgtaaata atgaagaaga agtaactagt aagatgatag ctatggtaaa
    31441 aaaattaaac cctgatgtag taatctgtgg accaagcttt aattacgaaa acttttctaa
    31501 gatgtcagca atactgtcta aaaatataaa tgataaaaca gatataccag cttttgcagc
    31561 tatgtcagaa gaaaatatag atgtgataaa cgaatataaa aatgacatat gtatagttaa
    31621 gactccaaaa aaaggtggaa ttggtcttaa tgattcatta aataacatat gtaaattggc
    31681 aaaagcaata gcaaataaag aagatataac tttgatgaaa gaagaatttt gttattaata
    31741 agagtattaa ttattagtaa gtttaaatat tatactattt ataataagca tacattagta
    31801 aaatctatat aatttaagga ggggaatttt aacatgtggg gaatgatagc gacatggaga
    31861 atggccctag aaggaataac tgaggcatca gaacatttag aaaaaggcat ggaagcaggt
    31921 gatgctgtag agcttgctgt aagaagagtt gaagactatc cattctataa atcggttgga
    31981 tatggtggac ttccaaatga aaattgtgag gtagaacttg acgctgcatt tatggatggt
    32041 aacacattag atattggagc agttgctggt ataagagatt attctaatcc tgtcagcata
    32101 gctaggaagt taagttatga gaaggtaaat aattttttag ttggaatagg tgctgaagat
    32161 tatgctcata aaaatggatt tgaaagaaaa aatatgttga ctgatagagc gaagttacat
    32221 tataaaaaga gaaaaaaaga gactttagac aaaggattaa gtccatatgc tggtcatgat
    32281 actgttggta tgatatcttt agacaaaact ggaaaaatgt gtgcagctac atcaacaagt
    32341 ggattgttta tgaagaaaag aggtagaata ggagattctg cactatctgg ttcaggattc
    32401 tatgttgata gtagtatagg aggagctaca gctactggtt tgggagaaga tttaatgaag
    32461 ggatgtatat cttatgagat agttagactt atgaaagaag ggaagcatcc acaagaagca
    32521 tgtgatattg cagtaaatga acttgataaa cagcttatca aaagaagggg aaaagctggt
    32581 gatttatcat tagttgccat gaataataaa ggtgaatggg gagtagctac aaatatagct
    32641 gattttactt tttcagtagt aactaaagaa gaatctccta ctgtatatat ttcaaataga
    32701 gtagatggaa agactataca taaggttata tcaaatgagt ggatggaagc atatgaggct
    32761 agaataaaag caccaataga agtaagtgag taaagtagtg gttacataat taaaatagag
    32821 ttttataata aggagatttt tatgaatatc ataagtaata aaatagatga gttaaaagaa
    32881 gatttattaa gtgatattat agatattgta aaaataccta gtgtcaaagg ggagtcagaa
    32941 aatggcttcc cttttggcga aaaagtaggg gaggcactta ataaagcatt agaaatatca
    33001 gaaaaattgg gatttaaggt tagaaacttg gataattata ttggttatgc agagcatgga
    33061 gatagtgatg attatgtttg tgtaattgga catgtagatg ttgtacatga aggagatggt
    33121 tggaagcatc agccttataa gggagaagaa actaatggaa gaatttatgg acgaggagta
    33181 ctagacaata aaggacctat aatgtctgcc ttatatggat tatatgctat taaagaatta
    33241 aatttaaaat tagataagag agttagaata atatttggta caaatgaaga aagtggattt
    33301 gaagatatac catattattt agaaaaagaa aaagcaccta ttatgggatt tacaccagat
    33361 tgtaaatatc ctgttgtata tggtgaaaaa ggcatggcaa agattagaat aaaaagtaaa
    33421 atcaattatg aagaagatgt atacttaggt tttatagaaa atatgtctga aaatgtgctt
    33481 gttacatata aagaactgaa tatagaaaat agtgatacta tattagatat aaaggtaaaa
    33541 tacgatttta gttataaatt gaaagatgta ttggatgaaa taaaagctag ttttccaaat
    33601 agtatcgata tagaggtaat ttctaatttt aatcctgtat attttgataa agagagtaat
    33661 cttgttaaaa agcttcagct agcttatgaa agggttacat cattagatgg aactccagta
    33721 acaactaatg gaggtacgta tgcaaaagta atgccaaata tagttccgtt tggaccttca
    33781 tttccaggac aaaaaggaat agctcataat cctgatgaat acatggatat agaagatata
    33841 atattaaatg caaagatatt tgcaaatgca atatatgaat tagcaaagga gtagttatgt
    33901 tagaaataat aggaatgtca gtagaagacg ctaaaattat agaagattgt ggtgcagata
    33961 gaatagagtt agttagtgct ttaactgaag gtggattgac accaagtttt ggtcttatag
    34021 aaagtgttgt taacagtgta aagatacctg tgaacgtaat gataagacat catgcgaaaa
    34081 gttttgtata cagtaaagaa gatataagta taatgcaaaa agatataagt gtggttaaag
    34141 agattggtgc taatggagtt gtatttggtg tattggataa aaacaatagt atagatgaga
    34201 aaaatctaaa tgttttattg aaatgttgtg ataatcttga tgttacattt catagagcaa
    34261 tagatgaatc aaatacaata gatagtgtta aaattttgaa ggattatgat aaaattacga
    34321 atatattaac atcaggaggg aaaggaagta tagttgataa catacaaatg ataaagaata
    34381 tgatgcttag ttctaatcac ataaaaattc ttttaggtgg aggtcttaat tttaataata
    34441 tagaaaagat aaaggaatta actaaggcaa gtaattttca ttttggtaca gcgattagaa
    34501 taaataacag tccttttgaa gatatagata gacaaaagct taaacaactc gtaaatataa
    34561 tttcaagata gtatatgaat ataaaatact tagtttaaat aaagagagta agttaattat
    34621 aatttgggta tatgaatgtt aataggtatt aaatatgaaa ttattgttga gtatgcttta
    34681 taacttttaa gtagttttaa atataaataa tgaaagaaaa gatagattat ggaggtttta
    34741 gagggtttat atttattaaa aaattataaa ggtatattgg catataatac acataatttc
    34801 ctaaaattac catcttatga catgaattca catattaata aacaattaat ggagattttt
    34861 gtggatagaa ataggagtaa aaatagcttt aaataaacaa aaacaagagt tattaacagt
    34921 tttatccaca ttatccacag gtgtttgtaa taatattaat ccacataagt atcaatgtaa
    34981 ctaaaaatga taaataattc tatcaattga atagaaaaaa tcataaaagt aaataataaa
    35041 aagaggatta tgagagttcg tgtagaatat attattaaag tataaatttt cttatactgg
    35101 cataattgaa gggagatgta tttatgaaca taattatatc tggaaaacaa atcgaattaa
    35161 ctgatgcaat aaaaaataaa atcgaaacta aactaagtaa gttagataat tacattcatc
    35221 cagacactga tgtaaaagtt acagtaagtg cgagaaaagc aagacaaaaa atagaagtaa
    35281 caatagctac tgtaaatggt cctataatta gagcagaaga tatgcaggaa aatttataca
    35341 cagctataga tgttgtatat gataaattaa gtaaacaatt aaaaaaatat aaaaagagat
    35401 tacaagataa acataaagac aacaagagta taagattcca agatgcagag ataaatctag
    35461 ctgatgaaca agaggattat ggggatgaaa atcaagaatt agtaattcaa agacgtaaga
    35521 aatttagtgt taagccaatg agtgaagaag aagctgtatt acagatggaa ttaataggac
    35581 atgattttta tatgttcaag aacatagatt cagatgaaat agcagtagtt tataagcgta
    35641 ataatggtgg atatggtata atagaacatg aataaagcaa tttagattta atcatattag
    35701 ctatcgataa ataaattatc gatagctttt taaattaata ttaaaagtat agtttttatg
    35761 gtaaaatgaa taatagtaca ataaagaacg aggagagatg taaaatatga gctttatgga
    35821 taatttattc aacatggcag ataaaaaaga attaaaaaag ttcaataaaa ctgttgatat
    35881 aatagattca ttagaaccta aatttgaatc tatggcagac tcggaactta aaaatatgac
    35941 aaatatattt aaagaaagat tagcaaatgg tgaaagtata gatgatatac ttccagaagc
    36001 ttttgcagta gttagagaag tttctaagag agtgttagga cttagacact acagagttca
    36061 aatgattgga ggtatagtac tacatcaagg aagaatagca gaaatgaaaa caggtgaagg
    36121 taaaactttg gttgcaactg ctcctgttta tttgaatgcg cttactggaa aaggtgtaca
    36181 tgttgtaaca gtaaatgatt accttgcaaa acgtgataga gaccaaatgg caaaaatcta
    36241 tgagttttta ggaatgagtg taggcgttat tatacacggt caaaatccaa aagttagaaa
    36301 agaacaatat gattgtgata taacttatgg tacaaataat gaatatggat ttgactatct
    36361 aaaggacaat atggtaatac acaaagagca aagagttcaa agaggattaa attatgctat
    36421 agtggatgag gttgactcta tactaataga tgaggctaga acaccactta tcatatctgg
    36481 acctggagac aaatcaactc acttatattc agatgcaaac acatttgttt taaccttaaa
    36541 accagatgat tatgaattag aagaaaaaga taaggctgta tctctaacag catcgggtat
    36601 acaaaaagca gaagtgtatt ttaatgttga taatataaca gatatatctc atacagaatt
    36661 ataccatcat ataaatcaag ctttaagagc acatgtaata atgaagaaag atgttgatta
    36721 tgttgctaaa gatggagaaa tagtaatagt agatgagttc acaggtagac ttatgtttgg
    36781 tagaagatat tcagaaggtt tacatcaagc aatagaggct aaagaaggtc ttaaaataca
    36841 aagagaatct aaaacactag ctactgtaac attccaaaat tattttagaa tgtacaaaaa
    36901 actttctggt atgactggta cagcaaaaac agaagaagaa gagtttaaag caatctacaa
    36961 aatggatgtt ttccaagtac ctactaataa gcttatgata agggaagact taccagattg
    37021 tgtatacaaa agtgaaatag gtaaatttaa tgctgtagca caagaaataa ttgaaaggca
    37081 caaagtcaat caaccaatac ttgtaggtac agtatcaata gaaaaatcag aattattatc
    37141 acaaatacta aagaaaaagg gcataaagca cgaggtatta aatgctaagc atcatgataa
    37201 agaggcagaa ataatagcac aagctggtag acttggagct gttacaatag ctactaatat
    37261 ggctggtcgt ggtacagata tagttctagg tggaaatcca gactttttaa ctaagagaga
    37321 aatgagaaga aatggattta aagaagaaat agtaaataga gtagatactc caatagaagg
    37381 tatacctgta aaaggtaatg aaattctatt tgaagctaga gaagagtatg aaaaactatt
    37441 tgaaaagttt aagcaacaaa cacaagaaga acaaaaacaa gttgtagaag caggtggact
    37501 tgctataata ggtacagaaa gacacgaatc tcgaagaata gataaccagt tgagaggtcg
    37561 tgctggacgt caaggtgacc cagggagttc aagattctat ataggtcttg atgatgacct
    37621 tatgagattg tttggtagtg atagaatatc tggtattgta gataaaatag gtcttgaaga
    37681 agatatgccg atagaacata gaatattatc taagtctatt gaaggagcac aaaagaaagt
    37741 cgaaggtaaa aactttggaa taagaaagca tgtacttcaa tatgatgatg ttatgaataa
    37801 acaaagagaa ataatatatg cagagagaaa acgtgtatta gaaggtgaag atttacaaga
    37861 acaaatacaa tcaatgactc attctataat agaagaagca gttactcttt atacacaaga
    37921 taaaggattt gatgaagaag gatttaaaga acatatgtat aatctgttct tgccaaaagg
    37981 tagcatagaa ataccagaaa tagaaaaact taatccagtt gaaataacag aaaaagttta
    38041 tgaaatagct atgaagatat atacttcaaa ggaagaacaa gttggctatg agagaatgag
    38101 agaagttgag agagtaatct tacttcaagc tgttgataac cattggatag accatataga
    38161 tgctatggac caattacgcc aaggtattgg tcttcgtgct gtaggtcagc aagacccagt
    38221 tattgcttat aaaatggaag gttttgatat gtttgatgaa atgaataaac atatcaaaga
    38281 agatactgta agatatttat ttaatataac aatagaaact ccagtggaaa gaaaagcagt
    38341 tgtagatgtt gaaaatctat cttctccatc agatggaaca cttccaacaa gtaaaactgt
    38401 taaaaaagat gaaaaggttg gaagaaatga cttatgccct tgtggaagtg gtaaaaaata
    38461 taaaaactgt tgtggaagat aatctcggag gattaaatgt taaagttaca agagtataga
    38521 catagtatgg agcagatggc ttcaaattta gaagaattga gggtttctct ttgacatagc
    38581 ttcattgatg aaaaatatac aaattaatga agaaaagatg aataaacaag acttttggaa
    38641 tgataatgaa gtcgctcaaa gagtacttca agaaaataag tctttaaaag agacattaga
    38701 agaatatgaa agcttaaaaa gcttactaga agatatagaa gtattaattg aaataggttt
    38761 agaagaagat gatgattctg tagaaagaga tatagaaaag tccatagaat caatggaaga
    38821 aaaattatct gaaatgaaaa taaagactct tctaaatgga gagtatgata aaaataatgc
    38881 tatattatct ataaatgcgg gtactggtgg cttagatgct caagattggg ctcaaatgct
    38941 tttgagaatg tatataagat ggtcagaaag taagggttat aaagttaagc tcttagatat
    39001 aatatcagac cctgaagcgg ggataaaaac agctacaatt cttgtagaag gaacaaatgc
    39061 ttatgggtat ttaaagagtg aaaaaggagt tcatagatta gtgagaatat ctccatttga
    39121 cccatcagga aaaagacata cttcatttgc atctatagat gtaacaccag agttagatga
    39181 aaatatagaa gtagaaatta atccatcaga tttaaagata gacacatata gagcttctgg
    39241 agctggaggg caacatgtaa atacaactga ttcagcagtt agaataacac atatccctac
    39301 tggtgtagta gttcagtgcc aaaatgaaag gtctcagcat ctaaataagg acagagctat
    39361 gagactttta atggcaaaat tgatagaatt aaaagagtta gaacaaaagg aaaaaataga
    39421 agatatacaa ggcaaatact cacaaataac ttggggtagt caaattaggt catatgtttt
    39481 tcaaccatat aaattggtta aagaccatag aacaaatgct gagtttggaa atgtagatag
    39541 tgtaatgaat gggaatatag acttgtttat taatgaatac ttaaaaatga ataaaatagt
    39601 ttaataaata tgcactgtaa ttaagatata tgtataagaa ggttagtgct taatatgcac
    39661 ttacagcact taggctgagg acgataaccc tcagccttcg ttctgttttg ttatacttat
    39721 gaaaaactaa cattaggagt gaataaattg gatataaatc aaattttaaa aaaagagttt
    39781 aatcttagag atgaacaaat aaataataca ttaaaactta ttgatgaagg aaatacaata
    39841 ccatttatag ctagatatag aaaagaaatg actggcgaaa tgagtgatgt tacactaaga
    39901 gaattttatg aaaagttaat gtatttgaga aatcttcaaa gtagaaaaga tgatgtagtt
    39961 agactaatag atgaacaagg taaattaaca gatgaaataa cacaaaatat agaaaaagca
    40021 aaaacactac aagaagtaga agatatatat gctccatata aacaaaagaa aagaactaga
    40081 gcaacaatag caaaagaaaa aggattagaa aaattagcat tgtcaatact agagaataat
    40141 ttagataata tagaaataga ggcaaagaat tatcttgatg aagagaaaga agttctttct
    40201 attgaagatg ctctaaaagg tgctagagat ataattgcag aactagtctc agatgatgct
    40261 aagattagaa agtatataag agaattagct ttaagagaag gtatgatagt tagtaaaagt
    40321 gccactgatg agaaatcggt ttatgacatg tattatgatt atagtgaagc agtaaaaagt
    40381 atgcctccac atagagtttt ggcaataaac agaggagaaa aagaaaactt cctaaaggtt
    40441 aagttagaaa taaataatga taaagtttta aattacataa taaacgaata tgtaaatgat
    40501 aaaaatttca aaaataaaga agaaatcgta agttcaatag aagattctta taaaagatta
    40561 attttcccat caatagaaag agaaataaga aatcatttaa cagaaatagc tcaagaaaga
    40621 gctataagtg tatttggaaa gaatgttaaa agtttattgc ttcaaccacc agttaaagac
    40681 aaagtagtta tgggatttga ccctgcattt agaactggat gtaaaatagc tgtagtagat
    40741 aaaaatggta aactactaga ttatacaaca gtatatccaa cagaccctca aaatgatgta
    40801 gagggagcaa agaaagtttt aaaaggattg attgaaaaat acgatataga tataatttca
    40861 attggaaatg gtactgcatc tagagaatca gaaacttttg tatcagaaat gataaaagaa
    40921 atagataacg aagtgcagta tgtgatagtt agtgaagcag gagcatctgt ttattcagcc
    40981 tctgaacttg caaatgagga acatccagat ataaatgtat caataagagg agctatatct
    41041 atagctagaa gattacaaga cccattagca gagcttgtaa aaatagaccc taaaagtatt
    41101 ggcgttggtc aatatcagca tgatttaaac aaaaagagat tagaagaagt actagatgga
    41161 gttgttgaag attctgtaaa tagtgttgga gtagatttaa atactgcatc atactcccta
    41221 ctagaacatg ttgcaggaat aagtaaaact atagcaaaaa atataatagc atatagagaa
    41281 gaaaatggag attttacgtc aagagcacaa ttgaaaaaag taaagagact aggaccacaa
    41341 gcatttactc aatgtgctgg atttatgaga atcttagagg gtaaaaatcc actagataat
    41401 acaggtgtgc atcctgaaag ttatgatata tgtaaaaaaa tgattgagat aattggatat
    41461 tctttagatg atgtaaagaa taagaatata tgtgaaatag atgaaaaaat aaaagaaatt
    41521 ggcttgagag aattaagtga aaaactggaa gtagggcaag ttacgttaaa agatatcata
    41581 actgaaatca aaaaaccagg aagagaccca agagaagaag gtataaaacc tatacttaga
    41641 actgatgtct taaaaattga agatatacaa gagggtatga ctttaaaagg tacaataaga
    41701 aatgttgtag attttggtgc atttgtagat attggaataa aaaatgacgg attagttcat
    41761 aaatcagaaa tgagtaatag ttttgtgaaa gaccctatga gtattgttac agtaggtgat
    41821 atagtagatg taaaagtcat tggaattgac ttaaacaaaa aaagagttgc cttatcaatg
    41881 aaaaaataga taagtttatg tatggttata gtacttaaat tctatgtttg taaatgaggt
    41941 tactcgagtg tataaatgtt aaaaatttgt aaaagtatac agtaagaaaa tatattaaaa
    42001 taagaacgat agtcttgaaa atcaggagga aaatgaatga tttttataaa aaatggtaaa
    42061 ataaatacca taacaaatgg gataattcat ggagatatac ttatagatga aggaaaaata
    42121 atagaaattg gagaagattt gatagctcct ttagatgtag aggtaataga tgctagtaat
    42181 aaacttgtgt ttcctggatt tatagatgct catactcatt taggtttgtg ggaagatggt
    42241 atagggtttg aaggagcaga tggaaatgaa gagactgacc caataacacc acaattaaat
    42301 cccatagatg gaattaatcc aatggataga acttttaaag aagcttttga aggtggaata
    42361 acttctgttt gtacaacccc tggaagtgca aatgttatgg gtggtcaatg tatagctata
    42421 aagacatgtg gaaaaagaat tgataaaatg gtaataaaaa atccagttgc atctaaaatc
    42481 gcttttggag aaaatccaaa gagctgttat ggtcaggatg ataaatctcc tcaaactaga
    42541 atggctatag ctgctttact tagagaaaac ctaaaaaaag cagaagaata tttagaggat
    42601 attgatatgt atgagagtca tgatgatgaa gactgtgaaa aacctgaata tgatataaaa
    42661 atggaaagtt taataccagt tttaagaaga gaaattcctt ttaaggcaca tgctcataga
    42721 gcagatgata tgtttacagc aataagaatt gcaaaagaat ttaatttaaa gcttacatta
    42781 gaccattgta cagaaggtca tcttattgta gatgaattag tggaagaagg gtttcctgta
    42841 atagtagggc catctttgtc agaaaggtct aaatttgagc ttagaaattt gacatttaat
    42901 acagcaggaa tactttcaaa tgcaggtctt gatgtatgta taatgacaga ccatccagta
    42961 ataccagttc aatatcttcc aatatgtgca ggtattgcag tcaaacatgg tatgaaagaa
    43021 gaaaaagcaa tagaatcaat tacgataaat ccagctaaga ctttaggaat agaagataga
    43081 gtaggctcta tagaagttgg taaagatgca gacctagtaa tttgggataa ttctccacta
    43141 gagattcaaa gtaatgtact ttacactata ataaatggca aagtagtgta tgagaaaaaa
    43201 taacttgaca attattaaaa aattaactaa tataagagtg taaggaaaat taaaaaatca
    43261 aagaagatct tcggggcagg gcgaaattcc ctaccggcgg tatagcccgc gaactactta
    43321 agtggttgat ttggtgagat tccaaagccg acagtaaagt ctggatgaga gaagatattt
    43381 atggaagata aataagttta ttttagtttg tttaaactat atccataaaa actcgaagat
    43441 agggtcttcg agttttttgt ttttcctaaa aaatataaac atattgatta gggggaaata
    43501 attatgcaaa acacagccaa aagaggcaat atgatttcaa caagaacttt agtagtaatg
    43561 tcaattttat ctgcaatatc ttatttatta atgtttattc aagcaccaat accaggtata
    43621 tttccagatt tcttaaaaat tgacttatca gatattccag caatattcgg aggaatgtct
    43681 ctaggaccat ttgttggatt tggaatagtt gtagtaaaaa atgtgttaca agcaataaca
    43741 gcaacaacaa ctggaggaat aggagaattt gcaaatatat taattggtgg ttcttatgta
    43801 atgatacttt gttattcata taagagaagt ggagatttaa aaggtgtatt gattggtttt
    43861 gtattaggaa caatatctat gactataatt ggttcattag taaattattt tgtaatgttg
    43921 ccactttatg gtcaacttat gggactagat gctataatag gtttaggttc agctataaat
    43981 cctcaagttc atgatttatt cacttttgta atttggatga tagctccatt taatgtgctg
    44041 aaggctgtta tactatcttt agtaacattg ccactttata aaaaaatggg aaatgtaata
    44101 aagaaataat tagacaagaa aatataaaat taaatgtatt gaatagccat aatttattta
    44161 taacttcagt cctatattgt gattgatata ttaaatatat tatggcttta ttaatttatc
    44221 aaaaaatgta tataaaataa gaaatattta gacaaattta aattttaaaa taaaggaaga
    44281 attaaaacga ttaactatta aaatatgaaa aaggtatctg caatatacag agcgaattta
    44341 taataaataa aaaataaagt tattattaaa aaaatataaa gattagtaat caaattttta
    44401 ataataacta atttaataat gatgggagtg tatattcatg aacttatcta aaaagataaa
    44461 aaaattattt ttagatgata aagggtatta ttttaatagt aattggaaag atattcatat
    44521 tggtatttat aatacaaaat taggagattc tttaaagtat atcactaatg aatatattga
    44581 aaatctggaa agggtaaaat atgactatcg tgaattaaaa aatatagaaa atcaaactga
    44641 tggtatgtgt attttagcaa taaggcagaa ttacaaggca ttagagtatg taaaaaatca
    44701 aaccgaagaa ttatgtttag aagcaataaa gtataattat aaagcacttg aatatgtaaa
    44761 agagcaaaca gaatatatat gtcttgaggc tataaaaaag gattgtaatg cacttaagta
    44821 tgtacgaaat aaaacagaaa ggcttattat aaaagtaata cgtcattctt ccaatataga
    44881 tgttgtaagt atactaaagg ctttagagac tcaaacaaga agaatatgtt tagaagcaat
    44941 taaaaaagat ggcaggtgtc ttgcatacgt tagagaacag agtgaagaat tatgtataga
    45001 ggccattaaa caaaattata aagcattaaa atatgtagaa aatcaaacgg aaaaaatgtg
    45061 tatagaatcc gtaagacaaa atggtatggc attacaatat gtaaataaac aaactgataa
    45121 aatatgtata gaagctgtca aacaagatgg aagaagtcta caatttgtca ataataagac
    45181 agaagaaata tgtataaatg ctataagata tttaaataaa aaatataata ttaaagatgt
    45241 actaagttac atagataagt atacagaaga tatatgtata gagattgtaa gacaaaatgg
    45301 aaaaatgctt atgtatataa aaaatcaaac agaaaaaatg tgtattgaag cagtaaaaga
    45361 gaactataag tctcttaagt atgtaaaaga gcaatctgag agaatatgta aagaagcatt
    45421 gaagcaaaac cataaagcaa aagagtatgt aaaaattgct attgatgatt gtatataagg
    45481 tgtagataca gccatcaata gtgaaagata aaaaaagata aagacaagta ctttataata
    45541 atctaggctt atactataat taattaagca gtgatataaa ttcacatgac gattatatca
    45601 aaatggagat gaattgatgg caaaaatata tttagaaaat gaaaataaaa ctagagaaat
    45661 aggatataaa ttaggtaaac ttttaaaaga aggcagtgtt atctgtttag taggagattt
    45721 aggagctggt aaaactacaa tgacacaaag cttggcagat tcattaggga tagaagatta
    45781 tataacaagt cctactttta ctataataaa tgaatatgaa ggaaaaatac ctttgtatca
    45841 ttttgatgtt tatagaatag gtagtagtga cgaaatgtat gatattggtt atgatgaata
    45901 tgtaaattca aatggaatct gtataataga atgggctaat ctaatagaag atatacttcc
    45961 aaaagaatat ttgaatatag aattgagata taaagatgac ggaagagaaa tgatattaac
    46021 tccaaaagga gagttttata aagagatagt tgaggagttg attaaataat gaaaatacta
    46081 ggtatggata cgtcttctat ggcagcaagt gtagcagttg ttgaagatga taacttaata
    46141 tgtgagttta cagtaaacaa caaaaaaact cattcacaaa agcttatgcc aatgatagaa
    46201 aatatgttga gtatgagtga tttaagtata aaagatatgg atttattagc tgtatgtata
    46261 ggtccaggct cgtttactgg acttagaata ggtatggcca cagtaaaagc tatggctcat
    46321 gtaaataata taccaataat agcagtaaat tctttggaat ctctagcaaa taacataaat
    46381 ttctgtgata gaaagatatg ttgtatatta gatgcgcaga aaaatcaagt ttattcagct
    46441 aaatacaagt ttgaaaatgg agagatgata gaattagatg gtgtagatgt tgtagagttt
    46501 gatgctttag tagatgaaat tgtatcaact aatgaagaat ttataatagt tggagaagct
    46561 gtatacaaat ataaagataa gttagagaat ataaaaaata taaaaatacc ttcaccagct
    46621 aataatgtat ctaacgcagg aagcttatgc tctttagcat taaataaata taataaaaat
    46681 atagatgtac atacttgtta tacgataaat cctatgtata tcagaaaatc tcaagctgaa
    46741 gtacaatatg atgaaaaaat gaagaggcta aataatggaa gataatacaa aactaatagt
    46801 agatgatata aaaatagaag aaatgactac agaagatata gatgaagttt ttgaagtaga
    46861 aaaaaattgt tttgaagatt attggtcaaa agagtctttt agaaaagagt tgtctaatga
    46921 agtagcaagg tatatagtag ctaagttaaa tggaaaaatt gtcggatatg tgggaatttg
    46981 gcttatactt gatgaagggc atataaataa tgtggctgtt catagtgact atagagggaa
    47041 aaaaataggg gacaagttga taaaaggcat agttgattta tgtaaagata ataatattgc
    47101 atctatgaca ttagaagtta gagcatcaaa taaaatagca caaaatttat atagaaaata
    47161 tggatttaaa atgggtggaa taagaaaaga atactacagt gataataaag aagatgcaat
    47221 tataatgtgg aaccaattaa aagaggtgta gagtttatga gtgatataat aacattagct
    47281 atagagagta gttgtgacga aacagctgca tcagttttaa aaaatggtag agaagtactt
    47341 tcaaacataa tctctactca aatagagaca cataaaaaat ttggtggtgt agttccagaa
    47401 gtggcttcaa gaaaacatgt ggaaaatatt gatatagttg ttcaagaagc tttagacaaa
    47461 gcaaatatag gttttaatga tatagaccat atcgcagtta catatggacc tggactagta
    47521 ggtgctttat tggttggtct atcttatgct aaagcacttg catatacttt gaatattcca
    47581 cttgtaggag taaatcatat agaagggcat ctaagtgcaa actatataga gcataaggat
    47641 ttgaaacctc cttttataac attaatagta tctggaggtc atactcactt ggttgaagta
    47701 aaagattatg gcaagtatga aattttagga aaaacaagag atgatgcatc tggagaagca
    47761 ttcgataaaa tttcaagagc aatgaattta ggttatccag gaggacctat tattgataat
    47821 ctagccaaga agggaaataa acatgctata gagtttccaa gagcctattt agaagaagat
    47881 agttatgatt ttagttttag tggattaaaa tcttctgtat tgaattatct aaatgggaaa
    47941 agaatgaaga atgaagaaat agtagttgaa gatgtagcag catcttttca agaggctgtg
    48001 gtagaggttt tatctactaa ggcactaaaa gcagtaaaag aaaaaggata caatatcata
    48061 actctttctg gtggagttgc ttctaattcg gggcttagag ctaaaataac agagcttgct
    48121 aaagacagtg gtattactgt taagtatcct cctctaattt tatgcacaga taatgcagct
    48181 atgatagggt gtgcaggata ttataatttt ataaacggga aaacgcatga tatgagttta
    48241 aatgcagttc caaatttaaa gataaatcag tagagttaga taaatttgta aatataaaag
    48301 tattgtttca aaaaagtaag cagaagtgaa attaaatata ataaaaatca atatagtata
    48361 tattaaaact tgaagtaaaa ctatttataa tctttatatt ccaatgaatt gcaacacatt
    48421 ttaggatata caggttgtct ttggcttaga tttgcttcaa gtttaatttt ttctatagaa
    48481 agattatttt aaaaagtggg taatataccc ttttttttca ttcttaaaaa gttttatact
    48541 aattatagaa agattaagga ggggaagaaa tgagtcaaag taaagaagac aaaataagaa
    48601 gtattttgga agcaaaaaat atcaaatcaa attttcaaaa taaagaaaat ttaagtgaat
    48661 ttaatgaaaa aaaagcaagt aaaagagcag aagacttatt agatgtatat tataatacat
    48721 tatctactgc agatatggaa tttccatatt ggtataatag agaatataga aaatcagatg
    48781 gtgatatacc agtagttaga agagctaaag ctcttaaatc tgctttttca catatgacac
    48841 caaatataat acctggtgag aaaatagtaa tgcaaaagac acgccattat agaggttctt
    48901 ttccaatgcc atgggtaagt gaaagtttct ttgttgctca aggagaacaa atgagagaag
    48961 aggcaaagaa attagccagt aacacagctg atgaacttac aaaatttggg tctggcggag
    49021 gaaatgttac tgaaagtttt ggaaatgtcg tttcaatagc tggtaaattc ggaatgagaa
    49081 aagaagaagt acctgtactt gtaaaaatgg caaaagaatg ggttggaaag tctgttgaag
    49141 atttaggttt tcactatgaa aaaatgatgc cagattatga tttaaaagaa aacctaatga
    49201 gtacacttat atgtatgttt gattcaggat atacacttcc tcaaggtaga gaagttataa
    49261 attattttta tcctttaaat tatggattag atggaattat agaaatggca aaagaatgta
    49321 aaaaggcagt tgcaggaaat gcatctggag atgggcttat tggtatggac cgtctatatt
    49381 tttatgaggc tgttattcaa gttattgaag gattacaaac ttggatttta aattatgcaa
    49441 aacatgcaaa atatttagaa agtatagaga cagatttaga agctaaaaaa gaatactcag
    49501 atttagtgga gatattagaa catattgcac ataaacaacc tcgtacattt agggaagctc
    49561 ttcagttgac ttatacaatt catatagcat cagtaaatga ggatgctata tctggtatgt
    49621 ctataggccg ttttggtcaa attttatatc cttggtatga acaagatata gaaaaaggat
    49681 taattacaaa agaagaggtt atcgaattat tagagcttta tagaataaaa atcacttgta
    49741 ttgactgttt tgcttcagcg ggcgtaaatg gaggagtact atcaggaaat acatttaata
    49801 ctttatctat aggtggatta aaagaagatg gttctacagg agctaatgaa ctagaggagt
    49861 tattattaga agcaagtatg agatgtagaa ctcctcaacc tagtttaact atgttatatg
    49921 atgaaaaact tccagaagat ttcttaatga aagctgctga atgtacaaag cttggttctg
    49981 gttatccagc ctgggtaaac aactcaaatg gaacaacatt tatgatgaaa caatttgctg
    50041 atgaaggtat gactgttgaa gaagcgagag catttgcatt aggaggatgt ttggaaacgt
    50101 ctccagggtg ctggaaacag ttaactttaa acgggaagac atactctatt gcaggtggag
    50161 caggtcaatc tgctggctct ggagttcact ttatagctaa tcccaaaatt ttagaacttg
    50221 ttcttatgaa tggtaaagac catcgtatga atatacaagt atttgagcct cataataagc
    50281 cattagatac ttatgaagaa gtcatagaag tatttaaaga ttattataaa caagcaataa
    50341 atgttttaga aagagcaaat aacattgagt tagatatatg gagaaaattt gatacttcta
    50401 taataaattc actattaaag ccagactgtt tagacaaagg tcaacatata ggaaatatgg
    50461 gatatcgtta taatgcaaca ttaaacgtag aaacttgtgg tactgttact atggtcaatt
    50521 catttgcagc acttaagaaa cttgtatatg atgacaaagc atttacaata gaggaaatga
    50581 aggatgccat attaaataat tttggattta aagatgcatt agaagtagga aattattcta
    50641 tggcagacca agtaaaagta gataagactg gtaaatatga tgctatttat aaggcctgtc
    50701 ttgatgcacc taaatatgga aataatgatt tgtatgcaga taatattctt aaaaactatg
    50761 aggtatggtt atcaaaagta tgtgaagaag cacaatcttt atatgctaag aagatgtacc
    50821 cttgccaaat atctgtttct acacatggac cacagggagc tgctaccttg gctactgcag
    50881 atggtagact aagtggaaca acatattcag atggttcagt ctctgcatat gcaggaacag
    50941 ataaaaatgg ggtatatgca ttatttgaat ctgcaactat ttgggaccaa gcagtagttc
    51001 aaaattctca gatgaattta aaacttcatc caacaactat aaaagggcaa caaggaacta
    51061 aaaaattatt agatttaaca agaagttatt taagaaaagg tgggttccat attcaatata
    51121 atgttgtaga ttcagaaaca ttaaaagatg cacaaaaaaa tcctgacaat tatcgtcaat
    51181 taatggtacg tgttgctggg tttactcaat attggtgtga attaggtaaa ccaatacaag
    51241 atgaagtaat tgctagaaca gagtatgaag gggtgtaaaa tatgagaaag catagtgatt
    51301 gtatgaattt ttgtgcagta gatgcaacca aaggaatttg tagattatca aaacaaatga
    51361 ttaatttaga tgatgcagca tgtccagaga taaaagtaat gccaaaatgt aaaaattgta
    51421 aaaattttgt tgaagctaat gatgaaggta taggcaaatg tgttggccta gagaaagaag
    51481 actgggtata ttcaacattg aatgcaatta cttgtgaagg acatgtgttt aatgagtagt
    51541 caaaagcaat tagaaggcat gattttcgat gtacaaagct tttcagttca tgatggtcca
    51601 ggttgtagaa caactgtatt tttaaatgga tgtccactga gttgtaaatg gtgtgcaaat
    51661 ccagaaagtt ggactgtaag accacatatg atgtttagtg aattatcttg tcaatatgaa
    51721 aatggatgta ctgtatgtca tggtaagtgt aaaaatggtg cattgagctt taatcttgat
    51781 aataagccag ttatagattg gaacatctgt aaggactgtg aaagttttga atgcgtcaac
    51841 tcatgttatt ataatgcgtt taaattatgt gcaaaacctt acactgtaga tgaacttgtg
    51901 caggtaatta aacgtgattc taacaattgg agaagtaatg gaggagtaac atttagtgga
    51961 ggtgagccac tacttcagca tgaattttta catgaagtat tattaaagtg tcatgaggta
    52021 aatatacaca ctgccattga aacgagtgca tgtgtatcaa atgaagtttt taataaaata
    52081 tttaacgata ttgattttgc atttattgat ataaagcata tggatagaga aaaacacaaa
    52141 gaacaaacag gtgtgtataa tgatttaata ttagaaaata tatcaaacct tgcaaattct
    52201 gattggaatg gaagattagt acttagagta ccagttatct ctgggtttaa tgatagtgat
    52261 gaaaatataa gtgatattat atcttttatg cacaaaaata atttggtaga aattaatcta
    52321 ttaccatttc acagattagg agaatcaaaa tggattcaat taggcaagga atatgagtat
    52381 tctgataagg gtgatgttga tgaaggtcat cttggagaat tacaagatat atttttggat
    52441 aatggtatag cttgttatgt tggtcatgag acagctttct aaaattaaat acaagtttta
    52501 attaaaaaag gatgttacac taaaagctag agttacatcc ttttttatgt ggtttttatg
    52561 tagaaataat taagtaaagt tttatatatt ttatatattg tttatattgc tttatgtcca
    52621 taaatattgc tttaaatata ctttatatat tttttatagt ttaaattaca gttagagaat
    52681 atattacgtt tactttattg aagcagaagt tttgtggtaa aatactacta tgaaaaaatt
    52741 taatagaata tattttagaa acaactaaaa ataatagtta ggaggaagac atctatatga
    52801 acaaatcatg gagcaacttg attattaaga ataaagacaa aaaagtacgt tcagggtgga
    52861 ttattatagc tgttatggct accttttaca tatttcagta ttatttaagc atgatacttg
    52921 tggaaataat aagaaaaatc ctggttgcta caggggacat taatttaaaa acaaactatt
    52981 tttcagaata tgctaattgg ttaaatgatg ttgggttaca gattgtattt caaattctaa
    53041 cagattcact tatgattatt attcctataa ttgtatggag atttattatg aaaaatccta
    53101 ttagtgaaat gggattacat tcttatagaa ttaagaaaaa agaaggatat gtaggaatgt
    53161 tattaggaat atttaattgt acagtaatct ttattataac aataacaatt ggtggtggtc
    53221 atgttacttc atgggtacca aaaatttcac ctttaacagt ttcttgaata cttgtcttta
    53281 ttttagttgc ttttgcagaa gaaattttaa atcgtggttt ttttatggca gttttacgaa
    53341 gatgtaaaaa tatttatttt ataatgattg ttccatctgt tatttttggg ttgattcata
    53401 tatggaatcc tgatgttact tttttgtctg taattaatat tataattatt gggattcttt
    53461 tttcatatat gtttataaag tcgtcgaata tatggatgtg tataggttat cattttacat
    53521 ggaatgtatt tcaggggatt atatatggta tgcctgtaag tggacttcag gttccaggga
    53581 ttataactac acaaatcact catggtaatc tattaaatgg tggtatgttt ggtattgaag
    53641 gtggaatttt gactactttt gttaatctac ttagttttat atttgtatgg tattactata
    53701 gagattctaa gtatgatttt gttagtgata aagtaaatgt gaccaatatc aagaattagt
    53761 tgattataat aaattttaat aatatcttct aagcttaaag tattctgtag gattgttgga
    53821 aatttacaaa aatataaaaa taacttggat attctattta ctccaagtta tttttatatt
    53881 cttttgtaaa tagaaatgga acataaaaaa ttaaaacaac catagatact attagtatat
    53941 catggtaagt gatagttgat tctttagaat ttgtgataag aaaagcacta ttatttaaaa
    54001 tatgaattat agcacaaaac cagatgttct tagtttttat ataaagatat cctaaaaata
    54061 ttgaaattcc aactatatgt atcgaacgta atattaatcc taatattggt gtttcaggtg
    54121 tatataatgt aaatattagt ggtaagtgcc acaaactcca acaaatacct aatattataa
    54181 ctcctagttt tttaccaaat ttattaaaaa aaatattctg taaaaaacct ctccaggcat
    54241 attcttcact aaaaaaaata taaatagtag ttaatgatgt tattggagta aatataaatt
    54301 ttatgatacc actataatta ggttgagacc cttgtagtat taatataaat gatgttggaa
    54361 caattacaga aatcataaag taaataatta acaaagaacc tagtttaata ttttttatag
    54421 gatataaatt ttttttattt gatg
//
LOCUS       DAMJWS010000016        53223 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMJWS010000016 DAMJWS010000000
VERSION     DAMJWS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53223
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.016"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            57..866
                     /locus_tag="PC399_001035"
     CDS             57..866
                     /locus_tag="PC399_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2591909.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            1000..1239
                     /locus_tag="PC399_001036"
     CDS             1000..1239
                     /locus_tag="PC399_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591910.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1427..1603)
                     /locus_tag="PC399_001037"
     CDS             complement(1427..1603)
                     /locus_tag="PC399_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591911.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1657..1753)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2108..2782)
                     /locus_tag="PC399_001038"
     CDS             complement(2108..2782)
                     /locus_tag="PC399_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2591912.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(3002..3556)
                     /locus_tag="PC399_001039"
     CDS             complement(3002..3556)
                     /locus_tag="PC399_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2591913.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(3634..3999)
                     /locus_tag="PC399_001040"
     CDS             complement(3634..3999)
                     /locus_tag="PC399_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2591914.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4464..5249
                     /locus_tag="PC399_001041"
     CDS             4464..5249
                     /locus_tag="PC399_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2591915.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5346..6170)
                     /gene="pdaA"
                     /locus_tag="PC399_001042"
     CDS             complement(5346..6170)
                     /gene="pdaA"
                     /locus_tag="PC399_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2591916.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6391..9510
                     /locus_tag="PC399_001043"
     CDS             6391..9510
                     /locus_tag="PC399_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2591917.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9528..10127
                     /locus_tag="PC399_001044"
     CDS             9528..10127
                     /locus_tag="PC399_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF2591918.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10384..12513)
                     /locus_tag="PC399_001045"
     CDS             complement(10384..12513)
                     /locus_tag="PC399_001045"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF2591919.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12779..13027
                     /locus_tag="PC399_001046"
     CDS             12779..13027
                     /locus_tag="PC399_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591920.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            13193..14584
                     /locus_tag="PC399_001047"
     CDS             13193..14584
                     /locus_tag="PC399_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF2591921.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14699..16168
                     /locus_tag="PC399_001048"
     CDS             14699..16168
                     /locus_tag="PC399_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2591922.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            16233..16826
                     /locus_tag="PC399_001049"
     CDS             16233..16826
                     /locus_tag="PC399_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF2591923.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            17008..18354
                     /locus_tag="PC399_001050"
     CDS             17008..18354
                     /locus_tag="PC399_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2591924.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMFYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18536..18907
                     /locus_tag="PC399_001051"
     CDS             18536..18907
                     /locus_tag="PC399_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF2591925.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            18900..19589
                     /locus_tag="PC399_001052"
     CDS             18900..19589
                     /locus_tag="PC399_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF2591926.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            20109..20975
                     /locus_tag="PC399_001053"
     CDS             20109..20975
                     /locus_tag="PC399_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2591927.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21217..22026
                     /locus_tag="PC399_001054"
     CDS             21217..22026
                     /locus_tag="PC399_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591928.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22170..23234
                     /locus_tag="PC399_001055"
     CDS             22170..23234
                     /locus_tag="PC399_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2591929.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            23262..23981
                     /locus_tag="PC399_001056"
     CDS             23262..23981
                     /locus_tag="PC399_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2591930.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            24005..24589
                     /locus_tag="PC399_001057"
     CDS             24005..24589
                     /locus_tag="PC399_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF2591931.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24591..25940
                     /gene="pabB"
                     /locus_tag="PC399_001058"
     CDS             24591..25940
                     /gene="pabB"
                     /locus_tag="PC399_001058"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF2591932.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            25930..26673
                     /locus_tag="PC399_001059"
     CDS             25930..26673
                     /locus_tag="PC399_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF2591933.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26707..27408
                     /locus_tag="PC399_001060"
     CDS             26707..27408
                     /locus_tag="PC399_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591934.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            27434..28006
                     /gene="folE"
                     /locus_tag="PC399_001061"
     CDS             27434..28006
                     /gene="folE"
                     /locus_tag="PC399_001061"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF2591935.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            28070..28861
                     /gene="folP"
                     /locus_tag="PC399_001062"
     CDS             28070..28861
                     /gene="folP"
                     /locus_tag="PC399_001062"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2591936.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28872..29237
                     /gene="folB"
                     /locus_tag="PC399_001063"
     CDS             28872..29237
                     /gene="folB"
                     /locus_tag="PC399_001063"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF2591937.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            29230..29736
                     /gene="folK"
                     /locus_tag="PC399_001064"
     CDS             29230..29736
                     /gene="folK"
                     /locus_tag="PC399_001064"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF2591938.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29717..30502
                     /gene="aroF"
                     /locus_tag="PC399_001065"
     CDS             29717..30502
                     /gene="aroF"
                     /locus_tag="PC399_001065"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2591939.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30757..32547
                     /locus_tag="PC399_001066"
     CDS             30757..32547
                     /locus_tag="PC399_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2591940.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32565..33731
                     /gene="rpoD"
                     /locus_tag="PC399_001067"
     CDS             32565..33731
                     /gene="rpoD"
                     /locus_tag="PC399_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF2591941.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            33896..34585
                     /locus_tag="PC399_001068"
     CDS             33896..34585
                     /locus_tag="PC399_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2591942.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            34599..35696
                     /locus_tag="PC399_001069"
     CDS             34599..35696
                     /locus_tag="PC399_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2591943.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35759..36331
                     /locus_tag="PC399_001070"
     CDS             35759..36331
                     /locus_tag="PC399_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2591944.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36391..36858)
                     /locus_tag="PC399_001071"
     CDS             complement(36391..36858)
                     /locus_tag="PC399_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2591945.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37244..37720)
                     /locus_tag="PC399_001072"
     CDS             complement(37244..37720)
                     /locus_tag="PC399_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2591946.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37840..38931)
                     /locus_tag="PC399_001073"
     CDS             complement(37840..38931)
                     /locus_tag="PC399_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF2591947.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(38924..39313)
                     /locus_tag="PC399_001074"
     CDS             complement(38924..39313)
                     /locus_tag="PC399_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2591948.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39505..39951
                     /locus_tag="PC399_001075"
     CDS             39505..39951
                     /locus_tag="PC399_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591949.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            40215..40940
                     /locus_tag="PC399_001076"
     CDS             40215..40940
                     /locus_tag="PC399_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591950.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            40927..42171
                     /locus_tag="PC399_001077"
     CDS             40927..42171
                     /locus_tag="PC399_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2591951.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGQGTEFHIIFHKMYDSD"
     gene            42251..42928
                     /locus_tag="PC399_001078"
     CDS             42251..42928
                     /locus_tag="PC399_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591952.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            42929..45490
                     /locus_tag="PC399_001079"
     CDS             42929..45490
                     /locus_tag="PC399_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591953.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            45681..46955
                     /locus_tag="PC399_001080"
     CDS             45681..46955
                     /locus_tag="PC399_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2591954.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(47116..50157)
                     /locus_tag="PC399_001081"
     CDS             complement(47116..50157)
                     /locus_tag="PC399_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF2591955.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            50364..50663
                     /locus_tag="PC399_001082"
     CDS             50364..50663
                     /locus_tag="PC399_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF2591956.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(50901..53129)
                     /locus_tag="PC399_001083"
     CDS             complement(50901..53129)
                     /locus_tag="PC399_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF2591957.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT        21159 a         5741 c         8735 g        17588 t
ORIGIN      
        1 gagtaataat gttgatttaa aataaaaata agatttcgga ggagtttatt atataaatga
       61 aagattacta taaaatagga gagatatcta aaatatatgg aataggtaga gattcattaa
      121 tgtattatga agaaataggc attctaagac cagttagaga tataaatgga tatagaatgt
      181 ataatatatc agatatatgg aaattaaatt taataaaaga atttagaagc ttgaactttc
      241 caatgaagaa aataaaagaa tacctagatg acagaagtat agagtctacg aaaaatattt
      301 taaatgaaga actcgattta attgataaaa aaatagctga atttataagc cataaggaaa
      361 atattataaa aagattgagt tcaattgaaa gtgtaattca aaatacaaaa atagatgaga
      421 tagaggttgt atatatagaa aaaagaaaag ctttagaatt aaatgctgat ataaagaggg
      481 atgaagactt cgatttttta attcaaaaac ttcagaaaga atatgaagat agatttaata
      541 tacttggaaa caacaatatt ggttctgcct tttctactga agcaattaat aaaggtatat
      601 ttaatgagtt taaatctgta ttttgttttc tggaaagtaa tgagaaagta tataatatag
      661 tttttgatga aggatattat gtaactttaa attatactgg gagtaattct aataataaga
      721 tatatatgga gaaggtgttt aaatttatag aagaaaataa ttataagata attgctgacc
      781 ctatagaaat ctataagata gatatacatg agactggaat tgtagaggag tttgtaacgg
      841 aaattcaagt tcctataact aaatagctat atttcaatgg attagatggt aaatagaatg
      901 aaaaaatgtt gatttgccac tgtgttataa aatcggtagc aaattatatt ttaaattata
      961 agatagcatg aaaaaataat tgtttagggg gttataaatg tgaaatgttc taattgtgga
     1021 agtgtaaata ttgaaaaggg aattttaaca actggtggca tatatgctga aactattgga
     1081 ttaaaatata gaggagatgg aaaattagat aaagttataa ataaaacttt tccagtacaa
     1141 tctactatat attcagattt atgtttagat tgtggagaaa tagttagaac ctatataaag
     1201 gataatactg ataggaattg gtgtaaagat aagaaataga ataatttttg aagtatatta
     1261 atatttgaag ttgaatttta ttaaagatac aagtgaaaaa gtataaatgg gaagaaattg
     1321 ttatatggaa gaaaactttg catatagggg agattagttt tttaatataa taacattttt
     1381 aaatgtagat aaaactaaaa agggttctct agaaaacaat ttctatttaa ttatacttac
     1441 ttaccaatac catcacttga tgtaaacaag attctactac attttctccg tactctagta
     1501 taaaatcatc tagctgtgta tctataaaat cacaataatc atataaacta ctgatataag
     1561 cagttaacat gtgtgtacat ttatccattt catctctaaa catatatatt actatccccc
     1621 taaaaaactt tcataaaaca aaaagaacaa tatctttttg gcaactggct gacataactc
     1681 atagtttatt ttataagcgt tagtccctat agctttgcgt cactaaattt ctctagtttt
     1741 gccgatttag ttttattcta caactaaaat aatacactag gataagttat tgttcaacat
     1801 acttgtcaga aaattgaaat atttaacagt atcttaacaa tatgaaatct atttttcact
     1861 caagaaataa actatacatg tagaatattt aataaaaatt atatttagtt ttgtattgaa
     1921 gtttttgtat taaaaagttt agttcacaag agttacttgg ctagataata gatgaacaaa
     1981 aatatcttga aaaaatatat atgaatggca aaaaacaagg gatgaatttt atatagactc
     2041 atcccgtaat tatttatata ttgaccttga ttaaatatat tattattttt aagcttcaat
     2101 ttttatatta tatttctaaa gtttctataa gaggattatc gctaaataat tttaataaat
     2161 ttgttttttt atatccttta ggtaacttag tatatataac tacttctaac caatccccat
     2221 caaggcgttt aatattcatg tttataattt caatattaga ttttttaaat ttaccttcaa
     2281 tatattctat ggaatcagtg ctgttagaaa ttttaagaga aacttgctct gctgtaggga
     2341 gtttcatcca tttcctatct ctatgaagtg ttatctgtac aataacaata atgatacttg
     2401 aaacaatacc aactaagtat aatccagagc caatggcaag acctgtacca gctgttgccc
     2461 atataccagc agctgtagta agaccactta ctgattgatt tctaataaat attaatccag
     2521 cacctaaaaa acctatacca ctgacaactt gtgctgcaac gcgggctggg tctagaccaa
     2581 tacctggagt acccactata tcattaaatc catacttgga aacaatgatt ataagtgctg
     2641 aagctaatgc cactataaaa tgagtgcgta ttccagcttc ttttagtcta ttctttctct
     2701 catatccaat taaagcccca cagaaaccag cagtaatgat tcttgctata tattcaaatt
     2761 gaatcaatat atgatggttc atttatctag cacccccttc tatataatat tatatatact
     2821 ataatactac attttttatg tgaatatagt aaagtttaaa atttatataa tagttatata
     2881 tgaatataaa atgtataaac gtatattatg tatataaaaa tgatagaaag catctaattt
     2941 taaatgatta aagagcaagc ttaaataaat caaacttgct cttttaacat agtgtatgtt
     3001 tttatttgtg atagttagat aaaaatttat ctgtgttttt tacatctgca aaactgttct
     3061 gtagtgatgc catgaatact tgatgtacag tatttgcatt aatttctata ttattccaag
     3121 ataaattctt cgttgtacat gcatcgctta ttagagttat gtcgtatcga agtttctttg
     3181 ctgttcttac acttgtatca atacacatat ggctcatcat tccacagatt actagattag
     3241 taatattttt tttctcaagg atactttgca aatctgtttc aaagaaacta tctggtgtgt
     3301 gtttagttat aatttcttca ttatctagtg gaaaaacaat tttatttatt tctgaaccat
     3361 gagtattagg aagaaaaaaa gaagctgttt tatctgttga tatatgctgt atatgaataa
     3421 ctggtaaatt gttttttcta aaaaacagta gtattttttt agcattttca gcagtttctt
     3481 ctgattgaaa taattcacat tttccatttt taaaataatc attttgaata tcaatcaata
     3541 ttaaagctat agacatgatt atacctccta tttattttta ttataatact attgtaaaaa
     3601 atgtaaatta cttacttttt ggtatgtata ggattactta ttgtagcaaa ttcctttatt
     3661 atctagaatt tcttgttgac catgttcaag catatattcg attcctattt cttgcattat
     3721 ttccactgct tttaacattt ttttaccacg attagtaaga aaatattcaa catgtaatgg
     3781 atatccagca gaacttattt tatcaataat cccaaaatca cgtaactctt ttaattgttg
     3841 aatcaacatt ttttgattta ttccataaat attatgttgc aattcagaaa atgttttatt
     3901 agattgtctt aattcaaata taaggatagt tttccatttt cctttgatta tgtcatgtac
     3961 aatttcaaga agacatgtat attcagctct tattttcata gcatatcacc cccagtaaaa
     4021 tgataataaa ttatatcact tgtcttgtga tatgacaaat atatatgaac tgtatataaa
     4081 ttttatgttt tattaagttc taaatttctt ctattgtagt tcatatttag tacataaata
     4141 attagtaaga tataagtaaa taaatatata acatgactat agaaatttaa ctttaaaata
     4201 acttatgata atttagagga ggaatatata tgaatatatc aagaaaagct atgaaaataa
     4261 tagaattagc acaaaaaatt gcaaataaga gaggtatttc agtagaagaa gcttggagtg
     4321 aagcagtaac agaatataaa aataaatacg aacatattgc atagatattt aaaaatttag
     4381 aatatttggt agaatataat gattttaaat gatactataa attataaggt atatcattat
     4441 taacatagaa ggaggatatc tgcttgattg taaaaataga taatacttta gaaaaagaat
     4501 tttggcaata tgtatcacat gaagaaagtt taaatttatt cataattgga tacgttgaga
     4561 attatggatt tagctctcaa tatcaagata tatggtctca ggtagaagat ggaaatataa
     4621 cctctataat attaaaaaat aagtcaacac ttataattta tagttttaaa aataatttta
     4681 atataggaga aatgaagaat catataaaag atttagatgt agaaagcata agtggaaaaa
     4741 aatgtgtaat agatagacta ataagtaaat acaaagattt ttatgaaaaa ctagataata
     4801 aattttgtgt attaaaagaa ataaaagaaa tagatttttc caacatgaag gagtataaaa
     4861 tagaaaatgc tcaggaaaaa gatattgatg aaataggaaa acttttaaat aggtcagact
     4921 ataaagtaag taaaaattac atagaagaaa gaaaagttca tttaaaagag ggaaatgtaa
     4981 gagcatattt tattagaaat gatgatacta tgattagcac tgtgtcaact ggtatggaaa
     5041 ctagtttttt ggcaatggtg gtctctgtaa gtacagataa aagatatagg ggaaaaggac
     5101 ttgcaagtta tatggtttac aatttgagta aagaattgtt gttagaagga aaagttccat
     5161 gtctttttta taataatgat gtagcaggta aaatatatca taatatagga tacaaagaga
     5221 taaatgaatg gacaattctt tttaaatagc tataaaaaat aaaaaactct atcgagaatt
     5281 aaagtattta taaataacta tatttttact ataattcatg atagagtttt tttaaattta
     5341 tgatactatt ttttattatt taaataatca agtgttttaa gtttataacc ttctttttcc
     5401 caatgtgtaa gaacctcatc taatatctca gtatttgttg atgaatttgg atgcagtaaa
     5461 actattgcac caggatgagt tcttgaataa attttgtttt tggcaaattc atgagtaggt
     5521 tgtttctttt cataccaatc tacataagca aaactccaaa atattgattt atatcctaaa
     5581 tcttgtgtat attttaaaga ttgttcgcta aatttaccca ttggaggtct aaaatactta
     5641 ggcatttctt ttccagtaac atctttgtat gctttttcta cacttgtaat ttcttctttg
     5701 aatttttcaa aatctgtaat acctgccatt gatggatggc tttttgaatg attacaaact
     5761 aaatgtcctt ctttttccat acgctttatt aactctggat tgctttttat ataactttca
     5821 actacgaaaa attgagcttt agcattgtgt tttttaagcg tatctaataa ttttggagtg
     5881 tttccacttt catatccagc atcaaatgat agatatataa ctttttcatt gggatttcct
     5941 acataataag ctccgtattt tttaaaaaaa tcagcttctt ttataggtga tggctgttta
     6001 ccatcctccc tagggttaaa ataccaatca tattcatgtg tgtcgagtgt tggactagaa
     6061 atttgagttt ttgttttatc taatgattta aaatttatac tagcaattcc aaaaagtatt
     6121 agagcaaatg ctcctatcat aatgtatttt tttaggctat cttttttcac aatttcacct
     6181 cttatttaaa atttaaagta tatttcttat aatatagttt gaatatttat agttaaaaat
     6241 attcacatat acaaataata aatgaaagaa gtttactgta agatataaag ttgatattaa
     6301 ttatcttaaa taaagtgtat ataaatgata aaatataaag agaaatgatt gtaaataagt
     6361 taggctggtt taacttgaag ggatataaaa atggataata taataagtgt attgaatgat
     6421 gttgtttttt tagatataga agttagtgga ttagattgtt taaattcaga aattttagaa
     6481 gttggagctg tcaaggttaa agactggaaa atatatacct atgaatcatt aataaaaaac
     6541 aaatttgagg tacctgtaga agtattctca gtatgtaaaa atttagataa aaatgattta
     6601 gaaatagcta atgaaataga actggtggaa gatagacttg taaattttgt agaagatagt
     6661 ttcattatat gtcatgattt aagtctgaaa aaaaagtttc ttgagtatca tatgccaaag
     6721 ttaaaaaaca aatttattga tttaattgaa ttagcagtta ttttagaacc atatcataaa
     6781 gattatagct tagaatactt aaaaaatact ttaacaaatt gtaattctaa agtggaaaat
     6841 agagctttat cagatgctat tgatataata aatattgtaa attgcctttt agtaaaattt
     6901 aataactatg aaaaaactac tttggagcca ttgagtttta aaataaattc atatcttaaa
     6961 aaatttaatc ttccaacatg ggaatggagc aaatttttag aagaggcaaa ttatgattta
     7021 tcaaataata taaatataaa aaaagaatat aatatatttg actcaaaaga agaaaagaag
     7081 aaggaaagag aaaccttaaa aattttaaat gaagaagaaa aaaattatga agaattatta
     7141 aaatacaaaa ctatttggga gaataaagaa ggatttacat atgaatatag acctggtcaa
     7201 tatgagctta ctaaaacaat aagagagttg tttagaaata gcgaagatga agaaaaaatt
     7261 gcatgtatag aggcaccaac tggtataggt aaaagtgtag gatatttatt gcctgcaata
     7321 ttagaggcta gaattaataa aaaaagatta ttaatttcta ctgacacaaa ggaattgcag
     7381 atacaattaa taaataaaga tattccaaat gtgttagatt ctttgggtct aaatggaaaa
     7441 gtaagttacg gatatataaa gggaaaaaat aactatattt gtatagatag actggaagca
     7501 tatatagatg attatgagtc tcaaaaccct actaaaggag aaattttatc tttaatattt
     7561 ttgaagagat tagttgaagg tggcaagtat ggagatatag aagaaataaa ttatttagtg
     7621 tttgacaact tcaaggaaat aagtactcac ttaagaaatg taagttgtga cccaaatatg
     7681 tgcagaccta aaaaatgcaa aaaagattgt ttatataaaa acagaataga agaattaaaa
     7741 gaagaacata taacagtagt aaatcattca ctattagcta aatggccata taaggatgaa
     7801 aaacctttag aaaatataat agtagatgaa gcacataatt tgactgaaaa aggatatgat
     7861 ttcttttcaa gcattataaa ttcaaagtct ttaaggtatt tattacaaga aatttatcct
     7921 tacgagttta ttcaaaatag tagctttata tataaaaaat atagtagaaa tatgagaaaa
     7981 ataaaggctt ttgataaatt ttataatgta ttaaaaattg gaagagaaga taaacaaaaa
     8041 attgctagaa gtataaatct cattattgaa gagatagatt caatattaaa gtttggtaat
     8101 tgtaatgaat ataataatgt aagtaattat aatttaagat gggaactgaa tttgcaaata
     8161 gacgaaattg tgggaaaact aaaaaaggat ggtatagata ctgaaataag ttatagagcc
     8221 tatagtgaaa agataaagtt gagttgtgag aaaataataa aaaatttagt ttcaataata
     8281 ataataattt ataggaatat tgatgatgat agtatagata aagaagcaga tatatataaa
     8341 tttggaaagg caaagacaag agatttagag gatataaaga ttatatttga aatcttttta
     8401 gagtatgatg aaaaggatga ttatgcaagg atagttgaaa ttgataagaa ttataatgac
     8461 tttgaatttc gagtagtgcc attaaaaata gcagatttat ttgaagaaaa tattttatct
     8521 caattagaaa aaggaatatt tttatcagca actttgagtt tatctgaaag tatgtcttat
     8581 tttaaaaata cattaggaat agacagagtt aagaatgtgg aaaagattat agaaccaatt
     8641 tttgattata aaaatagagt ctctgtagtt ggattttctg atatttgtga gtacagaaat
     8701 tctgaatttc caaatgagat gagtaaaatt atatctaata tatcaaagat tacagaaggt
     8761 catacattag ctctttttaa tagtaaagat agacaagaaa aaacttatga aatattgaaa
     8821 aaatacttac atagttttaa cttagaaatt tatgctgata aaaaggggat tagacacctt
     8881 aacgatttaa atagaaaatg tgtagtactt ggctcaaaag gatgttttga aggcgtagac
     8941 attccagggg atggtttggt ttgtgtaact ttagacaagc ttccgaatct aaatccaaaa
     9001 gaccctttgt attttactat aatgaagaag tatggtatag actactatac tataaattat
     9061 cctcaaatga ccataaaggt aaagcaagct atggggagaa ttttaagaag caaatatgac
     9121 tatggatgtt ttgtgatttt tgatgtagga actaatataa gtgtgttaaa gagattagag
     9181 aaagatttac acgattgtaa aatttcaaag gttaatagca atgagtttta tacttatata
     9241 aggcgacatt taaataaata tagaagttta atactaaaat ctgttatatt tgatactata
     9301 aaagcgttga atgttgatgc taaaatggat aacaatgatg ttgataagga tattataaaa
     9361 aaagatataa atgaaaatat aagacaaaga gctgtaaaag gagaagtgta ccatattgat
     9421 attataaaaa aagatatgaa agtaaaatat tttgatagaa actatcttat aaatttagat
     9481 atatttatga gagaagaaga taaaaattaa gagtgggagt gatatatttg gcaaaacaaa
     9541 agttttatgc tgtgaaaaaa ggtaaaaata taggtgtata taatacatgg gatgagtgca
     9601 aaaaacaagt aaatggattc tcaggagctg aatataagag tttttctact tttcaagaag
     9661 ctaaagaata tattgatggg tcagaaaaat tgagttttca agaagataag gagtttatag
     9721 aagcatatgt agatggaagt tatgaacatt cagtaaaaat gtatggttcg ggtgttgtga
     9781 ttttaaaaaa taatgaagtt ataaaaactt atagtgaaaa aggaaaagag aaaactttag
     9841 taagtatgag aaatgtagct ggagagatag aggcttctaa aattgcaatg cagtactgta
     9901 ttgacaataa tgttcaaaac ttgattttat actttgacta tgaagggatt gaaaagtggt
     9961 gtactggagt ttggaaaaca aataaagaag gaactatagc atataaaaat ttctatgaca
    10021 gtattaagaa taagcttaat gtaaaattta caaaggttaa agctcattct ggaaataagt
    10081 ataatgagga agcagataaa ttagctaaaa aagctattgg tgtataataa aaaaatattt
    10141 atacataatt acatggtata agagtatatc taaattccta ctttataatt aaacaacttg
    10201 taattaaaca acttgtaatt aaacaactta taattaaata aattttgatt aaaaaagcag
    10261 attgtattat gaaaaaataa gtatctaatt tataaacaat taataatagt attaattaga
    10321 tacaacttat ttaatataat aaaatctgct ttttatataa ctataaaatt ttcctacatt
    10381 ctattagaat tgcccataaa taccttcaag ttcttctttt aatacttcag ggtcatttat
    10441 accaatagac atggttctag ccagtatatt tgcttggtca taagttgatg tccatgagaa
    10501 tacaccacct agacaatttc taagaacata ttgagttttt aagaatattg ataaagcatc
    10561 atcatatgac attgcaaaat ctccatcctt aactaaatat ggaacttgag cagtattatc
    10621 aaatctatat tcatatccat ttttatttat ataatccctt ctaagctcat cataagttct
    10681 tgttatagta gcacctaatc ttccataaaa tggtatacca agaaggattt tttcagaagg
    10741 cattccagca ttctcaagat ttcttaccat tgcatcaaca ctatatcctg gcaaagataa
    10801 gtctgaatca aaaagatttg cttgatgttt tctaccattt gggcctgttt cacctgctgt
    10861 aaaatcataa ctcataagat taaaataatc tattatagga gctattttat ctatttcagc
    10921 acttgaattt atatatcctc tatctcctgt tccagctaca ctaagccatt tatcatctcc
    10981 tataacatct cttatggcag ttagtaagag tgtaaagttt tctctatctt gaggtcttga
    11041 tgttattcca gatgcactac ttccaggata ttcccagtct atatctattc catctaaagc
    11101 atattcattt atcatctgat tgacctgtct tgcaaaatta tatctagatg taggtgttaa
    11161 tgctgcatca gagaatcctt cagctcccca acctccaata gctacaatta cttttaagct
    11221 aggtttttca cctttaagag caagtaattg atttaaaaat ctaggataag gaataaacaa
    11281 atttccagac tggtctatct cagcaaaagc aaatataact gcatctaata gttgagcatc
    11341 tacattagtt ggatttccaa gaacatattc tgctacaatt ggatttatat ctggacagtt
    11401 tgcattttca actattggat tgtatgggtc tgttatttgt ggtctaacta attttttaga
    11461 atcttttaaa gttttcatag ctattttttt agtatttttt tcgctagtat aatcgtagtt
    11521 tttataatta tgtttataga aatcttcata actactatct tctttgttat agctagaatt
    11581 tattttgtca caagattcat ctttatcata gttaaaattc atttcatcac aagacttatc
    11641 tttaaactta tcttgtttgg agttgtgaac tttgttagta tttttagtat gttttttctc
    11701 atgattgcaa tcatctttaa tatcagtctt gtgttcttgt ttacagtcaa gagctttgtt
    11761 caggtagtca tgatgttcat gttcacagtt acattcattt tcaatgtttt tagtatgttc
    11821 ttttttccag ctacaagtat tatcaatttt cttgctcact tcttcatcat tataattatc
    11881 tggtacaaaa cataagtacc agtccataca tgaatatttc ttattacaac tgttaactct
    11941 attttttaat tccttatagt ttttaggata aggtaaaatc actggcattc caggaaacca
    12001 gtttgcagga gtaactatat tatctctatc actagtctga agtgcatcta ctattctaag
    12061 tatttctgga atatttcttc ctgtagttag tggataataa agaatcgttc ttagaatttg
    12121 attattatct ataataaata cagagcgaac agttgatgta tcactcattg gttttgatat
    12181 catgccgtat aacttggcaa ttctcatatc tctatcttct ataataggaa atggaatttc
    12241 tacacctgta agtaaagaaa tattatacat ccaagctaaa tgtgaactgt tactatcaac
    12301 acttagacca attagttctg tatttctttt tttaaattcg tcataatatt tagcaaaaca
    12361 taaaaattct gtagtacaaa ctggtgtaaa atcaccagga tgtgaaaata aaacaatcca
    12421 attaccctta tagtcagaga gtctaatagg accatttgtt gtattggctt taaaatcagg
    12481 agcctttgac cctaaacttg gcaaatttgg catgtaaatc acccctcaat aaattatcat
    12541 ttattttatt ctattaatta atacatatta gtgttactat tttattaaaa gttaagtaat
    12601 ccttatgtca aaaaaagata tttattaaaa agatttaata ttttcctaat ataaatgaca
    12661 atagtaattg ctattttttt actaatttgg tatagtaatg gataaggggt agtgtcaatt
    12721 ttagtgatta tacgaggtga gtggattgaa aaaaattcta agaattttag ttatctccat
    12781 gatgatatgt tttgcaatga cttttgcatt agtgttaata aattcagtta tgggagtaca
    12841 tataggattg gattattact ttttgatggg aagtgtgctt acagtttgtt taacaattgg
    12901 aattgtttta tgcaatgcta gaaaaatatt tgatagcaaa gctatacaca agacaagaac
    12961 gaaaactgtc agtaataata aaaaacaaaa gagtcattct agggatataa aaagaaagat
    13021 atcataattt atgtaaagga aatatttatg tataaaataa tgattgatag gtaaaataaa
    13081 aacacgattt aacttgtgtt tttatttttt ttcaagaatt atgatataat aaccaataag
    13141 aaatttagtt taccataaaa tataaagatt attaaatgga gagggaacta taatgaattt
    13201 tagagattta tttgagaagg ctgtagattt tattgatgaa aaaaggaaat ctatagttgc
    13261 gatttcatta tcaacattag gtgtaattgc tttgataata gtatttttct taagtagtaa
    13321 cgagtttagt gtaggaaatg aagctaatga gttgttaaag attatagaga aaagacagta
    13381 ttctatagct gttgattact acacaagtat agaaaaaaag ttttctgatt caaaaatgga
    13441 aaggtttaat aagtctgttt ctaaaaaaat aaataaattg ctattaaata gtggtgataa
    13501 atatttagat ggagacataa gtcaagaaag ttttattgga ttaataaata ccgtgaaaga
    13561 attaaacaaa ataagtatag atgtggatga cttattagct caagctgata gagttagaga
    13621 aatgtataaa gaagaaaata ctacttatga tattgccatt aactacataa gtactgtttc
    13681 aatattaaat ggtatgggta gtaacctaga tgtatacaaa cagaatatag aaacaatcaa
    13741 agagtctaga gatgtttatg actcagcagt aaaagaccaa aaagcatata agtattatga
    13801 agctattgaa aagtataata aagtattaaa agaagatgaa aagtattaca gtatggctca
    13861 aaatgggaaa gaacaatgta ttgaagaaat gtatgattac tatataagta gagctgaaga
    13921 ggcaaatact tctggagatt atgaaagagc attacaatat attgagtatt taaaagacga
    13981 ttattcagat gatgaaaagg tacagagttt agaaaaaaaa tataaaaaga atttatcatt
    14041 gtatacttta acatctgatg acattataaa tgtaatagct aaaaaaagtg gcaaagacaa
    14101 ggcaaatcta tctataaatt cacttccaca aatgataaaa aataataaat actattatgc
    14161 tgaagtatat gagtatgata aattaataaa tgaagttttg gtaagtgcaa aaactaaaga
    14221 tttatattca tataaagatg gtaaaaaaga ttacaaagta gattatggtg atggatactt
    14281 tagaatatta gaagatggaa gttatcaatt tggtattaca aaagataaag ctaagtttgt
    14341 gttaactaat actcttgatg aaaaagaaaa taagtataaa aagattgaga tattagatat
    14401 agagaaagca gatagatatg taaaaagtaa aaagagttta gaagaattgt ttggaaaata
    14461 taaaaatatt tattattatg ctgtagtaaa taaaggctta tttagaggca aagaagttta
    14521 tgctattaat atttacaatg aaaaaatata tgccatatca gaaaatggac taaatgaata
    14581 ttaattaata atattaaatt aatatataat taaaaagttt ttggggtttt aaggcgcatt
    14641 atgtagtaat ataataatag ggatatataa ataattttat tgtaaggaga agtatgggat
    14701 gagtaatgtg aacaaaaagc tagttatagc tattggagct gtagcattag tagtaataat
    14761 ttttataggc ataatgtgca tgcaatttaa aggggagaaa atagcaaaaa acacatatgt
    14821 taatggagta aatattggaa aattaactaa atcacaagca aaacaggaat tagctaaaaa
    14881 atacaaatta gagaatgttg attttaatta caatgataaa agctggaaag taaagagcaa
    14941 agatttaaat ttatctactg acctggataa aacagtagaa aacgcatata atttaaatag
    15001 aaagagtgct ttctttggca atctatcaaa aactatttca gcaaattttg gcaaaaaaag
    15061 caacttagta gttgcgataa attatgataa aaataaatta aaagcagaaa tggaaaagat
    15121 agctaaagaa atagatgtag atgtaaaaga tgctacactt gatataagtg gtgaaaaagt
    15181 caaggttata ccagattctg atggtcttaa aatggatatt tctaagagta tggagaattt
    15241 tgataatcaa actaaaaaag gtaactataa aaatgagtta gtagtaaaag ctactccagc
    15301 aaaagtaaaa aaagagcaac ttgctaatat agatacaaat ttaggtacgt attcaacaac
    15361 atttaaaact tctcaaataa atagaagtat aaacataaaa ttagcaacag ataatataag
    15421 taatgtatta cttatgccag gagaaacttt ctcatttaat aaacatacag ggaaaagaag
    15481 taaggaaaat ggatataaaa gtgcacctgt aataatggaa ggtgagatgg aagaagatta
    15541 tggtggagga gtttgtcaag tttcatctac gttatacaat tctgtgctat acgcaggttt
    15601 agaaattgtt aatgtaaaaa atcatactat accatcaagt tatgttccaa aaggaagaga
    15661 tgcaactgtt gcagatagtg gaatagattt cttatttaaa aataatctaa agcatccagt
    15721 ttacataaaa aattacgttt caggaaatca aattgtttgc aatatatatg gaagtgcaga
    15781 agataaacaa aatataacaa tatcaacaaa attagatggc gtttcacaaa ctacaatgaa
    15841 aagagttaat gaccctacaa tgcctaaagg taaagaaaaa gtagacaaaa gtggaagaaa
    15901 tgcatattct gtatcaacat atagaacatt taatgatgca aatggtaaaa aaattaaaac
    15961 tgaaaaaata gctaattcat attatcctaa gaaggaaggt attatattag ttgggactat
    16021 ggaaccaaaa ccagaggaaa aaccaaatac agacgaaaat aagaataatc aaaatacaaa
    16081 caatcaaaac ccaaataatc aacaaaaacc agaaacacca ccaactgata ataagcctaa
    16141 tgagacacaa ccacaaccgc aagcttaaag ttctcaagaa taggatttat gtagagtaat
    16201 aaaaaatata aatctaaata ggagggatta ctttgaattt aaataatata acgaattacg
    16261 ttcaaagtat ttgcgagaat atttctaatg tcttggatgt agatgttact ttagttacaa
    16321 aagatttaac taggatagca ggtacaggaa tattcagagg taaaatagga gagacaatat
    16381 cagagaagtc agtgtactct cttgttttaa aagatgggaa atcctatgtt ataaataaag
    16441 aaatagaaga gaactgtaat aaatgtgcaa atagagaaaa ttgtaaagag ttagctgata
    16501 tatgtactcc cgttaaaatg ggagagcata tacttggaat cttgggtata gctgcgttta
    16561 atgaagaaca aaaagaaaag atactttcaa aagataagga gttaactgct tttgtaagga
    16621 gtatgtctga tttaatatct tttaaactag atgaacttta taaacaagaa gttaagacta
    16681 tagatgacct tgagagggaa gctatagaaa atgccataaa gaaatatgga agtaatacag
    16741 aaggtatgaa aaaagtagct gaggcattaa atataggtat agctacactc tatagaaaag
    16801 taaaaaaatt caacattaaa agttaatagt attgaaaaag aggtaacctt tttatgaaaa
    16861 gaagaggatt acctttttta ttatgagaat ggtggaaaaa tatgtatagt tttgacttga
    16921 catattaaat atgttatctt aaatatatat tttagagtgg aggtgagtat tattttagtt
    16981 aaagatataa aacaagacat agaaagcatg cctagaaaaa ttttagatat atctttgata
    17041 gaagataata aaagtgattt gacttttttt aatatagaag aagaagacgg tgatgaaaca
    17101 ttaaaagcat taaaacagag ttatgtgact acaaattata aagaagaaac tctattaaac
    17161 ttgcaaggag ctttaaattc aaagtcagat acaacgtcta aggagttaaa attagaatca
    17221 tataaaagat ataacaatgc attggattta gcatataaaa attatataac tagtgccatt
    17281 aatatggtta ataaagctct ggagataaat cctaaagaca ttgatatact aaatcttaga
    17341 ggtcttttaa aattattaaa atgtgatttt gctaaatcat ttgagagttt ttatactgct
    17401 ttatgttatg aaaataatca tatatctaga aaatatgtaa atctaatttc atctgaagat
    17461 tttaaaactt ttttaggaag atacaatcat tctataagat ttataaatga agaattaaat
    17521 tatgagtcaa tacatatttt agagaacata attgaagaag aacctgaact aatagagcca
    17581 tatgtaattt tatctttgtt atatgataaa ctaggtaatg taaaaaagag agaaggatat
    17641 ttagacaaac ttaaagagct agataaagat aatcctgtat ttgaaaaaaa tgaagaagaa
    17701 aaagaagata caagtaaaaa ggaagaaata aaaaaagtaa aaagaaagaa aagtattttg
    17761 ccatatatta tagttggctg tgttttaata ggtatgggga tatatttgat tcaaagcaaa
    17821 aagagaatag aaaacttgaa taaccaaata agtagtaaag aggaaaagtt aagtgaaact
    17881 gataaaaagt taggagaaac gagtaaaaaa ttagaaaaaa cgaataagga attaaatgag
    17941 gttaaaaata aagaaccaga aagagagata actgtagctg atgaggaaga tttatattat
    18001 caagctctaa atttaaagaa aaacaaagaa tatgaaaaag ccatagataa ttttaaaagt
    18061 gtagtttctt ctggtaaaac taaaaaatat atttctgagt caatatatca attagcaata
    18121 acaaataaat tattaggaaa taaggatgaa gctataaaat attacaaaaa atatataaat
    18181 acttatacta aaaatgacca gtattatgat gattcatatt acgagttggg tatgttttat
    18241 tatgataatg gcgatttaaa aaatgcacaa cagacttttt atagtttgag aagtgaagtt
    18301 ccagacagta tgtataacaa ttcaaaaata aaagaaattt taagtgaaaa ataggtttat
    18361 atagttaagt tttttgaaac aaccaagtat gtattttatt aaattaaaat aaaaataaat
    18421 ttaaaaagag attgtgtcaa attataactt tctattttaa agaaagatat ttgcttgttg
    18481 ataaaactac aaatatatga tttgaagcaa tctctttaat ttttggaggt ggaaaatgaa
    18541 agttttcaat caattggtaa taattttatc tatctgggta ataggagagt atataagttc
    18601 ctttatacaa ggaattatac ttattcctgg gagtatagtt ggtatgctat tattatttct
    18661 actactacaa tttaaagttt tagacttgag tagtatagaa aatgtaagtg gatttttcct
    18721 cgataatatg gctatatttt ttattccagc aggtgtatca cttataaagt cattagacct
    18781 aataagggaa aatgtattag tgcttttatt agtcatatgt ttaagtacac taatagtaat
    18841 gtatactact ggaattattg tggagaaaat gataaaaaag aaggaagaag gacaaaaaaa
    18901 tgtataatgt attacagaca ccagtatttg gtataatagt aacaatagtt tttttcaatt
    18961 taggaagata cattcaacga aaaacaagta atcctatatg taacccttta ttaattgcaa
    19021 tcgtaggaat aatattgttt ttaagcatat ctaaaatccc ttatgagaat tacaagatag
    19081 gagctgatag tctaaatttc tttttaggtc ctgtcacagt agtacttgca gttccacttt
    19141 ataaacaatt tgaattattt aaaaagcata tgtttgagat tattgtagga ataggttgtg
    19201 gtatagtgat ttcttttgta tctgtattga taattggcaa aatagctaat tcagatgtaa
    19261 gtataataaa ttcactaatt ccaaaatcta taacaacacc aatgggaata tctttagcaa
    19321 attctttaaa tggtgtagaa tcaataacag ttgtagccat aatttttaca ggtatttttg
    19381 gtggtatggt tgcttctaca gtattcaaac tggggaatat aaatcatcca gttgctaaag
    19441 gaattgctct tggaacatct gcacatgctt taggaactac aaaggctttt gaacttggag
    19501 aaatagaagg agctatgagt ggtgtttcta ttggagtctc tggtacaatt acagtaatac
    19561 taattccaat tataatgaat tttatataaa aaataaaatt aagatgagta ccttgaatac
    19621 ttaaaacaaa ataaaataaa aaaaatttaa aataaaaact gaacgaaaaa aatgcaaata
    19681 atgaaataaa aaaatcatgt actgaaatta taaaaagaaa aatttcattt aggaattaat
    19741 tataaagaga caattactaa acttgaattt gctaaaataa aataaattac tgctaaaata
    19801 gatatttaga aaaaaatact ataaatttac tatattgcaa gttaaataat aagcagtatc
    19861 atattatgct agaaaaagtt tcatttaagg cataaatgag atgatttgaa cgtttttaaa
    19921 gattagaata taaaagattg cactagacat atatgaaaaa gataatataa tagatgatgt
    19981 aatagatgac agttcaagaa attataaaaa aaatttattt gaaatattat ctattacatc
    20041 aggaataata tatgtaagag taagctttta aaattctaaa gatttagaaa gattgaaggg
    20101 agaaagaaat gagtgaagtt aagaataccc tacaaaaaca tccagtttta agatattcaa
    20161 caatgctatt tgggatgttg atgacatcta taggaataaa tgggttctta agaccagcac
    20221 atctgcttag tggaggtgct acaggtatag caacatctat aaactatttg acaaatataa
    20281 atgtaggttt attaacattt ttaatcaata ttccaatatt tatcttaggg ttcatatacc
    20341 ttgaaaaaga gttttgtatt tcaagtttgg taaatatgat tgtattttca ttattattag
    20401 gagcaactca ggaaataagt aatattatac ctatacatga tatattgctt caaagtgtat
    20461 atggtggtat attatctggt ttaggagtcg gagttgtatt tagaactagg tcttctcaag
    20521 gtggtacaga tataatagct gcaatactaa aaattaagaa gaatatagaa atgaaagaca
    20581 ctgctttagc aataaatggc ttgatagtac ttacaggaag ttttttattt gggttggatt
    20641 tagcattata cactcttata ggtctatttc taaatgcgta ttcaatgagt tttattaaag
    20701 atgcaatgaa ttaccaaaaa tctgttatgg taatgtcaaa tgaagtcgat ttaatagcag
    20761 aagatataat gaagagttta gttagaggtg taactttttt agatgcagaa ggtgcataca
    20821 ctcatcaaaa aaagaagatt atatatacaa tagtttcatc aaatgagata ccaaagataa
    20881 aagatatagc tttgaagtat gataaaaagg cctttatatc tgtaaatgat gttacagaag
    20941 taaagggaag agggtttaaa gcaaaagatt tatagaaaat tttacaagct gggagtttat
    21001 aatgtaaatt tgtatattat atctctcagc ttatttaaat ttagtatacg aaaataaagg
    21061 ttttattaat gatttgtgta tcactttaaa agatatcatt ttaaaattaa aattgaaaaa
    21121 attacttaat atgtagaaaa catgaccact attaggatat aataaattta atagatttag
    21181 taaaatacct aggaataacg ttggaggaat ggcattatga atttatctaa agaatatgtt
    21241 aatttgtatg ataattattt gaaatatata aatgaagtaa acaaagagat tagaagtata
    21301 aagaacatga agaaacgtgt tttaaaaagg ggcgattttg ctccggaaaa tgaagtatta
    21361 gaattggaag gaaaaacaat acgcgatata gatgatgtcg atacaaaaaa accaaaaaat
    21421 aaaatctata aattttcaaa tggggatgta tatgtaggga aattcttaga gggaaagatg
    21481 gaggggaaag gctcatatac tttttttgta gatgaaggaa cagttatgga gtacatagga
    21541 gaatttaaag aagataaaaa aaatggaaaa ggaaacttta tattttcaaa tggaaatgaa
    21601 tatataggtt attttgagga agatatgatg agcggtattg gaaatatgtt atatagtagt
    21661 aaagatgaat atattggaac ttggaaaaac ggaaaaaaag atggaaaagg tatttataag
    21721 tggagtgatg gatgtatata tgcaggtgaa tttaaaggtg gaaagatgga agggtacggt
    21781 atttgctata atagtaaagg ggaagtactt tatgaaggag aatggaagaa taatttgatt
    21841 catggtaagg gaacttatat atgggaaaaa ggtaaaaagt atataggtga atttatgcat
    21901 ggtaaaaaac atgggcaagg aactttttat ctaaataatg aattggttta tgaagggact
    21961 tggaaatttg ataagccatc tatatttgat agaacattag atgaaatatt ttccttgaga
    22021 ctgtagcttt atctaattag tagataaagc ttttttctaa tacttactgt aaataggtat
    22081 gaatgatatt gattagtata cactatcaat attaacttaa taaatgcaat attaaaaaat
    22141 tttgattgat tttaagaagg agagatatat tgaagagatt aattgcttta gtgttagtag
    22201 tacttattgt tttattttat agtcctgaat tattaaaaga aggatatgat ttaaaagaaa
    22261 agttttttca aagtgaaata tggaatgaat ttttagacaa aataaatata aatgataatt
    22321 ctaagaaaga taaaaaagaa aaaagtcaaa atttacaatc aaataatact gaaggggaga
    22381 acttaggaga gtcagatata aagaattttg aaaaaataag cttaggagat aatttaagct
    22441 atgttttatc aagtatcggc aaacctggta gaattgatat tagtgaatat ggttttgatt
    22501 ggtatgtata taatcaatat ggaaaagaat ttgcaatggt tggacttgaa aatgatgaag
    22561 tagtagcact ttatagtaat tctattaatt catgtgaaaa tcaagatata aaactaaatc
    22621 aagatagaca aacagtgaga actaagatta caccattaaa atataagaga aaaggaaata
    22681 ccagatatat aataaactca gaaaatcaat atgatataat aagtaaggaa ggtaagtata
    22741 ttacaatttt ctacgatatt catgaagaaa atagagtttg ttcatattta ataattgata
    22801 aaagcactga agatgagttt aaaaatttat atccagatga tagcgaagaa cttaaaaaat
    22861 gttttgaact tgaagttata gaccttgtaa atagtgttag aaatcaaaga gggttaaact
    22921 cattgagata cagtgaacag gcaactttga gttcaagaaa acatagtgaa gatatgagag
    22981 ataataattt ttttgaccat gtaaataaga agaatgaaac tccatttgac agaatgaaaa
    23041 gagaaggtat agtatatact tcagctggag aaaacatagc agcaggacag attaatgcaa
    23101 tatatgcgca tgaagcttgg atgaactctg aaggacatag aaaaaatatt ttggggaact
    23161 ataataatat tggagttgga gtaatatttg gaggaagcta taaaacatat tacactcaaa
    23221 atttttataa ataactaaaa tatttgaaag gaagttaaaa aatgacatca tggaaaaaaa
    23281 agacagtata taagtgctta atagcagtag ccttgttttg tggaatagtg ttaatatcta
    23341 attttagtaa agtttctgct ttgatgatgg atactaatgg aaatgttttg attaaacatg
    23401 gttcaaggga aaaaaagcta atagcaatta cctttgatga tggtcctcat cctaaagaaa
    23461 ctagtcaagt tttagatgta ttaaagaaat acaatgtaaa agctactttt tttatagctg
    23521 gaaaacatgc aaaatggtat aaagaaccat tagtaagagc aagtaaagaa ggtcacgaga
    23581 taggaaatca tactttcaat catcctgata taagtaattt aagttctagt cagatagaag
    23641 aggagattgt taagtgtgaa gacattttga aggaagtaac tgggaaaaaa cctacattat
    23701 ttagaccacc attcggaagt tatagagaaa aagacttaat agaaattgca aaaaagcatg
    23761 actataaagt tgttttatgg acaggtgtag atgtgaaaga ttggaaaaat ccaggagcta
    23821 atagcattgc agataaaata attaataaag tacaaaatgg agatataata cttttgcatg
    23881 attatgcaac taatgataca gttgaagctt tagatatgtt tattccaaaa atgattgaaa
    23941 aaggttttaa atttgtaaca gtttctgagt tgataaaata atttttaggg gggagattta
    24001 cagtatgatt cttatgatag acaattatga ttcttttgta tacaacctag tccaatatat
    24061 agaagaatta ggggaagcag ttgtagtaaa aagaaataat gaaatcaaaa taagtgacat
    24121 tgaagaacta aatccagagg taattgtgtt atctccagga ccgtgttctc caaaagaagc
    24181 tggtatttgc atagatattg ttgagcattt taaggggaaa aaacctatac taggaatttg
    24241 tttaggtcac caaactattg gacatgtttt tggaggagat attataaaag cacaacagcc
    24301 tgtgcatgga aaagtatata gtatcaacca tactaataaa ggagttttta ggggacttaa
    24361 aaatccttta aatgtcacta ggtatcattc actaatagtt gactccaata cagttccaaa
    24421 agaattagaa attacagcaa ttactgataa aggggaaata atgggaatta ggcataagaa
    24481 atatttaata gaaggagtac agtttcatcc agaagctatt ttgtcagagt atggtcatga
    24541 aatgcttaaa aattttataa cagaagctag agaaagggta catatgtaat atgataagag
    24601 agataaatac taaattaaat tcttttgaga tatttactat ttttagaaat gaacatgaca
    24661 gttttatatt agatagtgct atggataaag aaaaattggg aagatattct tttataagta
    24721 gccaaccatt taaggtatta aagtataaag atactgatga aaatccttta gaagttttaa
    24781 aagaagaact acataagtat agggttgtaa atgatactaa tcttccattt gtaggaggtg
    24841 ctgtgggata tttatcttat gatttaggta attacataga aaatttacct agaactgctg
    24901 tagatgatat tgagatgcct gatatgtatt ttggatttta taatcatgta atagttatag
    24961 accatcttgt gcaaaaaact tatatagcta ctccaaacat agatatcgaa ttagaagaaa
    25021 agataataga tgatatagaa caaagaatat taaaagaaga gaaaaaaggt atagatagca
    25081 tatgttatga agaaaaagaa gtaacaccta taagacttaa atctaatttt acaaaggaag
    25141 aatttaaaaa tgcagttcaa agtgttagag agtacataag acaaggagat atatatcaag
    25201 ctaatttaac acaaaggttc agtggagaaa cagaacttac aagctttgaa ttgtataggg
    25261 acttaagaag atttagccca gcaccatttg gggcattctt aaactttgaa gatgcacata
    25321 tattatcaaa ttctccagaa agatttataa gatgtgttaa taagagaata gaaactagac
    25381 caataaaagg tactcgtcca agagggaaag ataaagaaga agatttaaga cttcaacaag
    25441 aacttagaaa tagtgaaaaa gatagagctg aactacttat gatagtagat ttagaaagaa
    25501 atgatattgg gagaatatct aaaactggaa gtgttaaagt tccagagcta tttgtaattg
    25561 aaccatatgc aaatgtaaat cacttagttt ccacagttgt aggtgagtta aaagatgata
    25621 aagatgctac tgatgtaata aaagcaactt ttccaggggg gtctataaca ggagcaccta
    25681 agattagagc aatggaaatt atagacgaat tagagccaac gcaaagaaat gtatatactg
    25741 gttcaatagg ttatataggt tttaatggtg atatggattt taatatagca attagaacaa
    25801 taatcaaaaa tgacaaaaaa gtctactttc aagttggagg aggaatgact tgggattctg
    25861 acccagatga agaatatcaa gaaacacttg ataaagctaa atccataatg aaggctttga
    25921 ggggatatta tgaagaataa tgtaggtttt gatagtagtt taagtaaatt tggaattggt
    25981 ttgtttgaaa ctattagagt taaaaatgga atagcagtag atttagatat ccatatagaa
    26041 agaatgctaa gctcaataaa ttgtttggat ttagatataa actataaaaa agatttcttg
    26101 ataaatgaaa ttgttacata tataaaaaaa gaaaatgtca ttaataaagc acttagaatt
    26161 actgtttttg atgaaggtta taatatatct attagggata ttccatataa tcaagagacg
    26221 tatgagaagg ggtttaaatt agctatatct ccaatagtta gggggaatag tttaattcat
    26281 agacataaga ctactaatta ttttgaaaac atatatacca aaaatatcgc aaataagact
    26341 ggttataatg atggtatatt tttaaattca gaaggtgtaa tattagaatg ttctatgagc
    26401 aatattttct ttataaaagg agaaagagtg tacactccta gtgatagatt accgatatta
    26461 aatggaataa taaaaaaaag gattatagag atttgtgacg aattacatat agagttgata
    26521 gaaaatgaaa ttaatatatc agaaattagt agttttgatt ttgtttttgt cacaaatagt
    26581 cttatgggcg ctataaaggt tacagaaatt gacaaaataa aatttaataa agaaaatgtt
    26641 gtttttgata aaatagtgaa gttattagaa taaattaaaa tattaacaat ttgttggaga
    26701 caaaagatga taggttgtat ttatgaagta tacaatgaag agatgactaa aataatagaa
    26761 aaccaaaaca ctttggctgt atatttagtg ggttctagta aagatgtaga ttttactctt
    26821 tatgatgtag tgataaacga tattgatgtg ttcgtttttg taaaagaagg ggaaaaacaa
    26881 gagagaatac tgtttaaaaa aaagggaata gaatttgatg taaattactt ttcaaaagat
    26941 ggtgtaaaaa aattattatc aaatagggaa tatttctttg tgaaagaaat gaaagatgct
    27001 aaagtactgt ttgataggga gaatatatca catattataa aagatataag tagagatatt
    27061 tacttagaag gacctagtaa aatgtcatta gaggaaaagt cttttataaa gcaagatatt
    27121 ggagcaaaaa tattgaattt aaaaaataaa gaaaaatttg atgtttttga atatcatttt
    27181 ttgacgaatt tatatttaaa agatataata attgggtact ttaatataaa tgataaatgg
    27241 gtaccaaaag acaaaaaatt gctaaaagtt ttaaaaaaag aaaataatga gctttttgaa
    27301 ctagtgtcaa aagtaagtga aaattatgac tatcaaagac ttttagatgt ttataattat
    27361 atttttaaag aaattgagac aaaagaggtt ataaaactaa tcttctagtc acaaatcaat
    27421 ggggggagtt taaatgaata aagttgataa agaaaagata caacatgcag taagagaaat
    27481 actagaggca attggggaag acccagatag agaaggtcta atcgaaactc caaatagagt
    27541 tgctaggatg tatgaagaga tattctctgg tcttagtgaa gaaccaagag accatttaaa
    27601 agttcttttt gcagatgaaa aacatgaaga gttagtattg gtaaaagata ttccatttta
    27661 ttcatgttgt gagcatcatc tagttccttt ctttggaaaa gctcatattg cttatttacc
    27721 aaaaggagga agacttacag ggctttctaa acttgctaga gtaatagata cattggcaaa
    27781 aagaccacaa ttacaagaaa gaatcactaa aaatgctgca gatataataa tggaagaatt
    27841 acaaccttat ggtgtcttag tagtagtaga agctgaacat atgtgcatga caatgagagg
    27901 cgtaaaaaaa ccaggttcaa aaactgttac atcagcagtg agaggaatat ttgaaaagga
    27961 cattgcgtct agagccgagg ctatgagttt gattactatg aagtagtaat ttattataga
    28021 tataatcatt tattgagata aaatttaatt taagggagtg tcattaattg tgtttgatta
    28081 tggtaaaaga acttatataa tgggaatatt aaatgtaact ccagatagtt tttctgatgg
    28141 aggagatttt aataacttgg acatagcaat acaacatgca aaagatatgg ttgaccaagg
    28201 tgcagatata atagacctag gaggagagtc tacacgccca ggtcatagct atgtagactc
    28261 agacgaagag ctaagaagag taatccctgt aataaaaaaa cttaaacagg aattggatat
    28321 accaatatca atagacactt ataaggcaga tgttgcagaa gaagcattaa aattaggtgt
    28381 cactatggta aatgatgttt ggggtcttag aaaagataaa aatatggctt ctgtaatagg
    28441 aaaatatgat gcagaagtgt gtataatgca taatcaagat gggacaaact atgacaaaga
    28501 tataatggag tcaataaaag acttttttaa ggtaagtata gaaatggcaa tgagttgtgg
    28561 tgtaaaaaaa gaaaaaatag tacttgaccc aggtgtagga tttggcaagg attttgaaca
    28621 aaatattgaa gtattgagaa gattaaatga attaaaagat ttagggtatc ctattttgct
    28681 tgggacatcc agaaaatcag taataggaaa agtacttcca gttgagccta aaaaaagatt
    28741 agaaggtaca atagctacta ctgttcttgg tataagagat ggcgtagata tagttagagt
    28801 acatgatgtt tatgaaaatt taatggcagc tagaatgact gatgcaatat atagaaagta
    28861 ggtataatat aatggataaa atattactta gtaatctagg attctatgga tatcatggtg
    28921 ttcttaaaga agaaaatttt ctaggtcaga aattctttgt cgacatggaa ctttatattg
    28981 attcaagaga agcaggtctt agtgacgata taaataaatc tgtaagctat gccgaggttt
    29041 acaatgttgt taaagatatt actgaaaata aacaatttaa tcttttggag gctttagcgg
    29101 aaaatattgc tgaagaagtc ttgaataaat ttatacttat aaatggagtt atggtcagag
    29161 ttagaaagcc tgaggctcct gtaaatggta tttatgacta ttttggtgtt gagataagga
    29221 gaacaagaga tgaataaggc ttatttggga ataggtacta acatgggtga tagatttgat
    29281 aatttatcaa gagcatgtga acttttgaaa aatagtgatt ctatatataa agtaaaagaa
    29341 tcaagcttat acgaaacaaa accatgggga tatacagaac aagcagattt tctaaatatg
    29401 tgtgtagaaa tagaaacaga gtttgaacct tatgagttgt tagaatattg tcaagaaata
    29461 gagagagaac tacacagaga gagaatagtt cattggggac caagaactat agatgtagac
    29521 gtactatttt ttaatgatgt agtttccact gatgaaagac taacaatacc tcatccaagg
    29581 attcaagata gagctttcgt tttgatacca cttatggatt taaatgaaga acttataata
    29641 aatgaaaaaa caataaaaga acatttaaac ctcctatctg ctgaagaaag agaagaagta
    29701 aaggagcttg taggttatga aagaaaacct atttaataat aattcaaaga tagagataaa
    29761 tacaaataac aaaaagataa ctgtaaaaaa agataaactt ataatagctg gaccatgtgc
    29821 aatagagagt tatgagcaac ttttggagac tgctaaattt gtaaaaagtc agggagcaaa
    29881 tattttaaga ggtggagcat acaagccaag aacatctcca aattcatttc aaggattaaa
    29941 aaaagaaggt cttgaaatat taaaagcggt taaagacgaa gttggtatgg ctgttattac
    30001 agaacttatg gatgtaagag atatggacga actgtatagt ataagtgata taattcaaat
    30061 tgggtcaaga aatatgcaaa actttactct tttaagtgaa gtgggaaaac aaaataaacc
    30121 tgttatgtta aaaagaggaa tagcttccac aattactgaa tggataggtg cttctgaata
    30181 tatagcaata gaaggaaata gtaatattat aatgtgtgag agaggtataa gaacgtataa
    30241 tgattatact agaaatacat tagatttagc tgcagtaccc attattcaaa aggaaacagg
    30301 tcttcctgtt gtggtagacc caagccatgc cactggagtt agatatttgg taaaacctat
    30361 gtcactggca tcttttgcat gtggagcaga tggaataatg gtagaagtac atcctgaccc
    30421 agaaaatgct ttgtcagatg gtgctcaatc tctttgcttt aatgaatttg aagatttaat
    30481 gaaatcaatt aataattatt aaaagaggca agagcctctt ttttattcgt aatcgttgct
    30541 aaggtaaatt tataagttat aatttgatag ttagattatt taatataaat aaagatttaa
    30601 tattgataga attaatataa aaaataaaaa ttttttgatt aataagaagg atatcaataa
    30661 aaaaagtcga attgtgtata ttgagtgatt gacaaattta gaatatagtg tagaatagaa
    30721 atgttaacac aaaaaattac accagaaggt gataatatga atgatataaa agacattatt
    30781 gaagaaataa aagctagatg cgatatagca agtataatat ctgaatatat gaatataaaa
    30841 caatctggag ctaattacaa aggattatgt ccatttcatg gcgaaaagac accttctttt
    30901 tatataaata cgtcaaaaca gatttataag tgttttggtt gtggtgaagg tggagacgta
    30961 ataaattttg tcatgaaaat ggaaaatttg gattttatgg atgctgtaaa aattttagca
    31021 aataaatgcg gtatagaaat taacactaac atgaatgaag aaactaggat taaaatagaa
    31081 aaatctaaga aatttcaaga tattcatacg gaagcagcta gattttatct ttcaaactta
    31141 ttgggaagca aaaatcttgg gtatgaatat ttaagaataa gaggactaga tgataaaatt
    31201 attaagaaat ttggtttagg attttctttg gattcatgga attcacttat gaatactcta
    31261 atcagtaaag gatataaaaa acaagactta ttggagtgcg gtctaattgc aaaaaataga
    31321 gatggaacta attgctatga taaatttaga aatagagtta tgtttcctat attcgattat
    31381 agaggaaata taataggatt tgggggaaga gtattagacg attctttgcc aaaatactta
    31441 aattctccag acacgttaat ttttaataaa aaacagaact tatacggatt aaattttgct
    31501 agaaaaaatt tagaaagtaa aactatagtc ttagttgaag gatatatgga tttaatttcg
    31561 ctttatcagt atggtataaa aaatgtagta gcaacccttg gaacagcctt gactgaacaa
    31621 caaggcattt taataaagag atatgcggac actgctatta tatcttatga ttctgatgaa
    31681 gcaggaataa aagcgacttt aagagctata gatatactta ctaaattagg tatcaatgta
    31741 aaagtgttag acttaaaaga tgctaaagac ccagatgaat ttgtaagaaa atacggactt
    31801 agtgattata aaaaagctat ggatgtttct actcactaca taaaatataa aatagatcat
    31861 cttaaaaaag aatttaacat tcaaaaagat gaagaacgag tgaagttcgc aaaagaagct
    31921 tctaaaatta taaaacaatt gacaagtcct gttgaaattg atttttatac aaagtattta
    31981 agcaatcaaa tagatattaa tgtcgagtct ataaagagag aagtttatgg taaaaattac
    32041 aataagccat ataataacaa aaatcaaaag aaaatagaag aaaaagtaat tgaaaaagtc
    32101 gaagtcagac aagacggaaa acaattagtt gaagaaactc taataaaaat aatgttagaa
    32161 gacaaaaaaa ttagagaaat agcgttactt aaagtagaag aaagtgattt tttattgaaa
    32221 gaaagtaaag aaattttaaa ttacatgatt aaaaatcaag aattggacaa aataactatt
    32281 gacaaattga aaagtttaaa catatccgaa gaatacttaa aagaattaaa ttcaatttct
    32341 ttgaatagta taaacttaga gaacacaaaa gaaatagaag gaataataac aaatatcaga
    32401 aaaaactcat tagaagagca aattaatagc ttgttgagag aacaacaaga attagaaaat
    32461 aataatgata tgaaagaggt agatggtaga gttatggaaa ttgctttaaa aatagttgag
    32521 ataaataaaa ttttaaagag cttgtagaga aaggaggttt gagtgtggaa aataaatcga
    32581 ataaaaaaga gttaaaaaag gttactgcta agacattaat agaaaaagga aaaaagcaag
    32641 gttctctgac acttgcagag ataatggaag ctttttcgga gactgaactt gataaggatc
    32701 aagtagaaaa tctttatgag actttaggaa atttgggaat agaaataaca gaaacaaaaa
    32761 actataaagc tgatatagat ttttcggttg ctgatgacga tttaagtata ggccacttag
    32821 atgaagatgc agaggcaatt tcacatgatg actcttctgc aatagaaata gaaactgtgg
    32881 atttatcttt accaaaaggg ataagtatag atgaccctgt aagaatgtac ttaaaagaaa
    32941 taggaaaaat tcctctactt aaaccacatg aagaagtaga atttgctaga aggatgcacg
    33001 aaggtgatga gatagcaaaa caaaggttag ttgaagcaaa cttaagacta gttgtaagta
    33061 tagcaaaaag atatgtagga agaggtatgc ttttcttgga tttaatacaa gagggaaatt
    33121 taggtcttat aaaagcagtc gaaaaatttg actatacaaa aggatataag tttagtactt
    33181 atgcaacatg gtggataaga caagctataa cacgtgctat tgcagaccaa gctagaacta
    33241 taagaatacc agttcatatg gtagagacta taaataagct aataagagta tcaagacaac
    33301 tacttcaaga acttggaaga gatccaaaac cagaagaaat tgcaaaagaa atggaaatga
    33361 cagaagataa agtaagagaa ataatgaaaa tagctcaaga ccctgtgtct ttagaaacac
    33421 caataggaga agaagaagat agccatttag gtgactttat tccagatgat gatgctccag
    33481 ccccagcaga ggcagcagca tattctttat taaaagaaca aatagaagat gtacttggtt
    33541 cattaaatga tagagaacaa aaagtattaa agcttagatt tggtcttgag gatggtagag
    33601 ccagaactct tgaggaagtt ggaaaagagt ttgatgtaac tagagagaga ataagacaga
    33661 tagaagcaaa agcactaaga aaactaagac atccaagtag atcaaaaaaa cttagagatt
    33721 atttagacta aaaagataca tcattgatgt ttttgtgata aatgccatga gataagtgat
    33781 atctcatggc tattttgtag taaagattta attttatttt tgtagttatt tcagtctaat
    33841 atctataact tggattaaat cctataaata aagggaattg aaaggagaat tatatttgaa
    33901 attaacagat agattattga aaatagcatc attagtgtca gatggtaaaa aaatagctga
    33961 tatagggacg gaccacggat acataccagt atatttgtta aaagagggaa aagtaccatt
    34021 tgcagtgcta gcggatgtta ataaaggacc tcttgacaat gcacataaag aagtcataca
    34081 gaacaatctt ttagataaag ttgatttacg attgggttct ggtatagaga tacttgaaat
    34141 aggggaagta gaagaagtga taattgctgg tatgggtgga atcttgataa gtgagcttct
    34201 cgaggctaaa aaagaagtag cacataatgt agaaaaatta atattacagc caatgcaagc
    34261 acaagaagaa cttagatatt accttttaaa caacggatat gaaattttag aagaagtttt
    34321 agtgagagaa gattttagaa tatatgagat aattgttgcc aaatatacag gtaaaaatac
    34381 tataatagaa gatgagatat attatgaggt tggaataaag cttttagaaa acaaagattc
    34441 tctttttaat gattttatag aaaaaaagat aaagacatat agttctatag ttaataaatt
    34501 agaagggaaa aatggggaag ctatagataa gaagagaaag gaaagcgaag ttgcaataaa
    34561 aaaacttgaa aatttaataa aataaatagg aggtatatat gctattaaag agtctaacta
    34621 gaaaaattga aaaaaagtat cctctaaact tagcagagga ttgggataat gtaggtctta
    34681 tagttggaga ttttgatatg gatgtaaaaa aggtattggt atctttagaa gctaatgaag
    34741 atgttattga tgaagctata tccaaaaata tagatttaat tgtgacacat catcctttta
    34801 tatttgggaa aattaataaa attaatagtg gagatttaaa aggcagactt atccaaaaac
    34861 ttattaaaaa tgatatatca ctttattcta tgcatacgaa ttttgatata gcatttgatg
    34921 gtctaaatga ctattttatg gaaattatgg aatttggtaa ttcaaaggtt ttagatataa
    34981 ctaaaagcga aaatttgtat aaattagcag tatacgttcc acataactat tcagatgaac
    35041 ttagaaaggt tttaagtaat tcaggagcag gtcatatagg gaattatagt gattgtacgt
    35101 tttcaataga aggtgaagga caatttaagc cattagaagg ctctaatccg tttcttggaa
    35161 gtgtaaatga tatagaaact gtaaatgaag ttaaaattga aactgtagta ccacagaagc
    35221 ttttaggtgg ggtaataagt tctatgttag atgcacatcc ttatgaagaa gttgcatatg
    35281 atttgtacaa gttagaaaat aaaggtgaga tatttggact aggtagaata agtaaacttg
    35341 ataaaagtat gacattagaa agtctttcaa ggaaaataaa agagaaatta aatatgaaac
    35401 acataagagt agttggaaac cttagtacag acataacgaa agtagccgtt gtaacaggag
    35461 ctggttcaga atttgtaaaa aaagctaaga gacaaggtgc agaagtacta attacgggtg
    35521 atgtaaagta tcatgaggca caagatgcac ttgatattgg aatgtgtata gtggattgtg
    35581 gtcattttga tacagaagat atattcaaaa atgttatgaa gagattttta gatgaattta
    35641 gtgaaattga agtaataaaa agtaatgtat atttgaatcc atttagtact atttagcagt
    35701 gtttgataat aaaaatacta tataaaaaat aaatttgagt ttaattaggg ggataatgat
    35761 gaaagtagca gtaatttttc atagtgtttg tgggagtaca tatttattag ctagagaata
    35821 taaagaagcg cttgaagaaa tgaatatcga ggtagatata tttagagtaa gtgacgaagt
    35881 tgcaaaaaca cttcctcaat attatttgat aaattccaaa gaatacaaag acgaatttga
    35941 aagtataaat gtaattaaat ctggaaaaga aatattagat tatgatgcta tatttatggg
    36001 ttcaccaaca tattatggaa atgtatctgg accaatgaag atgtttatgg atagtttttc
    36061 tgatatttgg gtaggtactc ctttgagtgg taaaatattt ggttgttttg ctacagcagg
    36121 ttcacaacat ggaggaggag aattagcttt gcaagctatg aatatatttg cccaacatat
    36181 gggaatgact cttttgtcag ttccatgttc agtaagagga ggttatccag cgtatggaat
    36241 acttcatata gcaggagata attctgatat aagaccaaat gatgatgcca agataggtat
    36301 aagagattac ctaaaaagac ttaacatata agaatatcat ataaaaatac ctaaaagcga
    36361 ctgtatatac ttttaggtat ttttacttaa ttataaacgt gaagctttac cagaaatatg
    36421 gtcaatttct attttaacaa cagttgttaa gttcatatcc ttctctatgt acttcatgcc
    36481 ttcttcaaag aatcctttag aatatttttt tattatttct acaagagcat ttttcttttc
    36541 ttcattttct acagtgcaag cttttccaaa aactatagca ctagaataag tagtactaaa
    36601 tttatctgga ataacacttt cattagcaac tactaaaaaa gaaactttgt tgtttttcga
    36661 tatattatct aacttatgcc catttcttgc acaatgaaag taaatagaat cattaaagta
    36721 tacataattt acagcaactc cataaggata accattttca gatatagtgg aaaaagtacc
    36781 aaattcacca ttttttaata cttcaacagt atcttctttt gtcatttctc tttttttcaa
    36841 tctcatttct ttaaacataa tatcatcctt tctaattttg atgcttaaat taaattttag
    36901 aacatttata aagattatgc tatgtttttt acaaatttta agaaaaaaat atagtttaag
    36961 ataaaaggta gtgaaagaga agctatttgg aggtttattt atgatattat tgttagagta
    37021 gtataaacaa tactaataaa tttaatagct ggtttcttgt tatttaagat agtaagcatg
    37081 tctataagaa ggggaagtta tcgagggtag aagttgttat ttataactat attataaaga
    37141 aaatattatt atataaaata ataaatagta aagaggtatc aagttgaata taatacttaa
    37201 tacctcttca aaattaagtt ataaaagtgt tgtgggaatt gtcctataca tggtaaggag
    37261 ataataaaga atgtatcttt aaagtctcaa ctaaacagca aaatttatcc aacaaacata
    37321 caataaatgc ttctttgtat ctaggtggct taacagtaag tggccacatg tgttttaaaa
    37381 ttatgtccgt ttctttttca tttatttgaa aaaataaagt tgcatttttt aatgcttctc
    37441 tagggtgagt aaatccatgt aatccttttc tatcaccttt gtaatgccaa tcataaagaa
    37501 aaaagtcgtg tagtaaagct cctctaataa cactttgaat atcaacactt aagtgcaatt
    37561 ttttacataa tagataacta taatatgcta ctgacaaact atgttcaaga catgaaacat
    37621 tgccatgatg aggaaatgtt ctcataagtt gaactttttc agaagaaagt atctcagaag
    37681 cacattcatt aaaatatttg atttcagctt ttgacaacat tatgaaaatc tccattctta
    37741 gtaatatata aatactatac ttcaacgata atatgaaata tttcttatcc tacaaaatat
    37801 aatacccaat aatacaagta agataccttt tatggtattt taaagtaaga gagattttat
    37861 taggctaaac aatgaaaaaa ttaaaagtaa agctatagca atatatagta tttttccaag
    37921 aggattgcca aaatttatag tccatccaat accaaacctt ttattaacca ttaatgatgg
    37981 gtcatttgga ttattataca tgatgcctgc tatccaatac ttctcatctt cttctggaga
    38041 ataagatgag ttgaatttta aatttgggga attaatatat gttgctatta aataaattat
    38101 aactaaaagc gttgtaatat tcatagcagt tgatagtttt gtttttattg ataaaagagt
    38161 agtaaaaaac tgtgttgtca ttataatcat caatattaaa aacaaataac caattttatt
    38221 taaatattta atgttttcaa ctctactttc ttcaattcta tttgtatcta tcttaatcct
    38281 tgactttatg gatccaatgg aagttatata taataaaacc attaaacaaa agtcagtacc
    38341 gattaattta aatacattaa gaaatgattt atctataaaa acatcaggag acccatttat
    38401 gttccaatga gtggcaattg agtctggcaa ttgattataa ttaaatgcta taaaaatact
    38461 cattcccaaa gtaaatagta tcggtattaa atagattatt ttaaattttt ttattatttt
    38521 atttttctta tttataaaat ccatatctac tattaatttt gaatctatat ctatatgacc
    38581 taatttagaa taaatttctg atttaagctt cttagctttt ttatgtgtat gaatatatag
    38641 tatactttca tataataaaa aacctataat accaataaaa taggaaagtt ccaatttatt
    38701 aaatgtaaag accagaataa aaactaatat aaagtctata ataaaaccta aagataatag
    38761 ttttttaaat tctttatcta actttataaa gtaattataa tttttatgct cttggtctat
    38821 agaaactcca tagatgtgtt ttttacctac aaaatagggc ataaaataaa atgtagcata
    38881 catcaaaatt agaactggaa atattgtaaa tagacattca atcttactca taatatttca
    38941 cctcatactt gtcaaataaa cttgaactta gtgacaaaaa ttcttcttta ctaatacctg
    39001 ataaataaga ttttgccacc aataattcca agtcaagttt atttttttga cgagttgtat
    39061 tatctatttt cattggtagt tgagctacaa tagcaccttt tcttctatca atatttaaga
    39121 atccctcttc ttttaattca ttgtaggatt tgttcactgt atgtaaattt acaccaatat
    39181 tttcagccat acttcttaca gatggaagtg attcatttat tttcaattct cctgaagcta
    39241 tagccttagt tatttcttct ttaatttgta cataaataga tttatcacta ttaaaatcta
    39301 aatttaaaac cataaaaaac ctccattatg ctttataaat gaaaacgatt gttatataca
    39361 tagtataaca taaaaaataa taacaaacac aaaatataag tttatatttt gtaaaaattg
    39421 taatttaaaa aataacataa tttttattaa aataatacag atactattta tagatagaaa
    39481 taaaaattag aaaggagaaa aaatatgtta acatatttaa tgaaaagaca aatgcataaa
    39541 aaagatgatg gaatgagtaa accaatgatg attacaggag ctgtaattac aggtgtagga
    39601 gcatatgctg tatataaaat ggtaaaaagc cataaatcta attctcaaat ggtcaataca
    39661 aattattatg gaaatagcga ttatgatgat tataaatatg acgaatttga ttatgactgt
    39721 gattgtagag acacaaaaca agattatgaa tatgaagaat taaaaaaaaa agtaaaagaa
    39781 tttaattcaa gaagaataaa ttgtgaaaat tgtccacatg tttctcaaga agaaatgatg
    39841 caatatgtaa atagtgttaa agatgtaaag gaagagattg atttacatga agatgctaaa
    39901 aaagataata tttacaaaga agatgaatac aaaaaatatg aagaagaata agtaatattt
    39961 tgtataactg aacaaatagt aatgtcaaat tatttaatat ctagttttaa tatgtaagtt
    40021 gtaacttaac ttgaaagaat cagtatcatt ctttagatag ttaagtaaca acttacattt
    40081 tatttttata aattttaata gttctttgtg actattttgt aattttagaa atattttagt
    40141 atccagggta attgtgtata ataaaacttg tatagataga tagtatgaaa ttaagcagat
    40201 agtttaaaat taaaatgaaa agaaaggaaa tagttatgtt aaagttgttg atagttgaag
    40261 atgatagtac tatatctttt ggaataaagt atgctttaga acaagaagga tttagtatag
    40321 atatatcaaa ggatttatct agtggaaaag aaatgatttc ttctaatgaa tatagcatga
    40381 tactgttgga tgttacattg ccagatggaa ctggttatga gctttgtcaa tatataagag
    40441 gattctctca ggttcctata atattcttga ctgcttgtga tgaagaagta aatattgtaa
    40501 tgggtctcga tataggtgga gatgattata taacaaaacc ttttagaatt agagaattaa
    40561 tatccagaat aaaagctgtt ttaagaagaa aaggtaatac ttcagaagaa aataagaaaa
    40621 tacttaaatt tggagattta agtatatata cattagaagc tagagtatat aaaaatgata
    40681 aagaaatatt tctgacatca gttgaatata aacttctttt aattcttata cagaataaaa
    40741 atactgtttt aagtagaact caaatattgg aaaaactttg ggatgtaact tatgattttg
    40801 taaacgacaa tactctaact gtttatataa aaaggcttag agaaaaaata ggtgatgatt
    40861 tatgtgaacc aatctacata gaaactgtaa gggggattgg ttataaatgg ataggaagtg
    40921 aaaatagtgt ttctatataa tccagaagtg aagaagtttt taagtaagta tgttactttg
    40981 atgtttattg ttatcataat atctattgga tttagcataa tcaacgtgtc cttaactaaa
    41041 gagatggttg taagaaataa tcaggctata ataggaactt tatcaagcaa gtatccaaac
    41101 ttggaaagtc agatagtaga cataataact caagggaagt ctatggagaa catagattat
    41161 ggtcagaaaa tattaattaa gtataattat gataaatcta ttaggataaa ctctgagcct
    41221 attatttcta aattagtttt agagactatc aaaataaata taatattagt atgtataata
    41281 tttattttga tatttgtgtt ggttgttaga tattttaagt caatttacaa tgatttatca
    41341 gatatgacta agtatgttta ctatagttca gagggtaagc catttgatat gaaaaacaag
    41401 aatcaagaag ggcagatagg tcttctaaaa actgaactct taaagatgac aacaattctt
    41461 aatgaaaagg ttgaactttt aaaaagagaa aagatatttt taaacaatac aatttccgat
    41521 atatcacatc aattaaaaac tcctatgact tctttaataa tgttgaatga tttattatat
    41581 aatgatgtac catacgaagt aaaaatcgat tttttaaata agataaaaaa tcaattaaat
    41641 agaatggatt ggttgattaa aagcatgctt aaattatcaa aggtagaagc aaaagtgata
    41701 aattttaaaa aagataaggt aaaatttagt gaactcatac atagagcaat gctatctatg
    41761 aaaataccta tggagattaa aaatcaaaaa ctgactatag agggaaatga taatgtatct
    41821 tatattggtg atattgattg gtccgtggaa gcattagtga atataatcaa aaattgtgta
    41881 gaacatactc ctgaatttgg aaatataatt ataacctata aagaaaatcc attattttct
    41941 gaactaataa taaaagatga gggtgaaggt atagataaaa aagatatacc acatgtattt
    42001 aaacgattct atagaggaag aagtagttca aaagaagata gtgtaggaat aggtcttgca
    42061 atgtcaaaat ctataataga aagccaaaat ggcgatatat atgtaaacag cgaaaaaggt
    42121 cagggtacag aatttcatat aatatttcat aaaatgtatg atagtgacta atctgtaata
    42181 tttaattcac tttatagtaa tagccaaaac ctaatatatg aagtatagaa tataaatatt
    42241 ggaggtagtt atggaaattt taagagtaga aaatctaact aaaagttatg gtaaaaatga
    42301 aacaaaagtt gatgctataa aaaatgtaag tttatcagtt gaaaaaggaa cgtttatagc
    42361 aataacaggt ccaagtggaa gtggtaaaag taccttatta catttattag gaggagttga
    42421 cagaccaact agtggtaagg tttatataaa tgatgttgat atatataatt taaaaactaa
    42481 ggatttggcg atatttagaa gaagaaatat aggtcttata tatcaatttt acaacttaat
    42541 tccagtctta aatgtgaagg aaaacatatt gcttccagca gagcttgaca atagagatat
    42601 tgatagagaa tattttgatg atttaatgaa aacattaggt ctaattgata gagaaaagca
    42661 ccttccaaac caattatcag gaggtcaaca acagagaacc tctataggac gtgcattaat
    42721 taatagacct tctataatat tagcagatga gccaacaggt aatttagata gtaaaaattc
    42781 aaaggaaata ttagaattgt taaaattgtc agtaaaaaaa tacaaccaga cactaataat
    42841 gataactcat gacaaaaata tggcacttca agctgataga attataagca tagaagatgg
    42901 aatcataaaa agtgatgagg tgatgtagat gaatttatat acattactaa ccattagata
    42961 cttaaaacaa aataaaagaa gaaccattgt aaccataatc ggtataatac tttcaacagc
    43021 tttaatttgt ggtataggaa atatatttga aagttttatg gattatcaaa tgagagaaac
    43081 tataaagaat gatggaagtt tccatgttac attttatgat gtaaatagaa aaaatgtgga
    43141 atatgtaacg aaatcagctg aaatagaaaa acatgcattt accaaacaac ttggatattc
    43201 taaattagaa aatagtgaaa atgctatatt aagtataaag caatatgata aaaatgcaat
    43261 aaatggatat aaagtttcta taaaagaagg tagatttcca gctaaggaag gagaaattgt
    43321 tctaagtgaa agtattatta atcttataga tgataagtta aaaataggag ataagataac
    43381 tttaaaagta ggggatatgt ttgactcaag caagaaaaag attgatagta tgacatttca
    43441 tgaaggtgat tatatagcaa atgaaaaaga tagaactttt aaagttgttg gtataattga
    43501 aaaacctggg tttgaacgtt ataatggaat tgcaacagct atagcctact ttaatcctat
    43561 gaataattac aatgatacaa ttaatgtatc agtagcagta agaaatccta aagatgtgta
    43621 taagataagt aataaaataa gtaaaaatat atattcaaat aaagataaag aagcagtttc
    43681 tgatatgata aaatacaatg aacatttatt gaggttacaa ggtgctagta agtattcaaa
    43741 tataaataac tctataaagt ctataattat agcagtaact attttagtaa tcatatgtac
    43801 aattgctaca gtgtacaatt cctttagtat ttctataaat gaacgaaaaa aacagtttgg
    43861 gatattaaat tctataggtg ctacatctag tcaaattaaa agattagttt ttatagaagg
    43921 tattataatt agtttaattg gtataccaat aggactaata tctggaacag tagctataga
    43981 cctattgttt aaaattataa ataagtattt tactgaatcg gttgttacta agatgagttt
    44041 gcagatagtg tataatccaa taataactat tgttagtata ataatagttt tgcttacaat
    44101 ctttatatca attctactac ctgcaatatc ggcttctaat atttctcacc ttaatgtgat
    44161 taaaaatact ggagagtata aagtgggcaa aatcaaaagc tcaagactta ttaaaatgat
    44221 ttttaaaact gaaggagtac ttgcatataa aaatattagg agaaatagaa aaaaatttat
    44281 aataactctt ttttctttaa tggtaagtat aataatattt atatctttta gtggctttac
    44341 attactttta ctaagaggtg aagaaattag aaattctcaa agaaaatatg atttgtattt
    44401 aactgcaaag aacacagcta ggtctgtaga ggatactata agtgaactag aagatattga
    44461 tgggataaaa aactttgaat tggcaacagg acaatatgtg tctataagag taagtgaaaa
    44521 taaaataaat aaatccaagg aagatttaat caggaaatat tatcaaaaat ataagattgg
    44581 agatagttat gaatatgatt tcagtaataa tgaattaatt tttccaggag attttgcagt
    44641 aaaaaacaaa ataaataact tagtacaggg ctcttttaat aaagagagag caattgaaga
    44701 aaatggtgtt atattagtta gaaaaagtgc atttgaagaa aatgggaaga aaggtgtagt
    44761 agaacttacc aactataaag taggagatac agtaaattgt gaatatcttg atgaaaatgc
    44821 cttaagtaaa aaagtaaagg tcaagattct tgctataact gacgaagaac gtctaggtct
    44881 tggttatcaa aatatggggt tacaatttat aacttatgat gaagttgcta aaaatttaaa
    44941 tttaaaatta agtaaaagcc ttatatttat tgattcagga ggaagcatta agactaagaa
    45001 ggaaatagaa gctttggcaa ataaaaataa ttttaacttt tatgatgaaa gtgcaagcaa
    45061 tgaaagtgaa aaacaagatt taaaggttat taaaatattt gtatatggtt ttatagtagt
    45121 gatatcttta gtgagtgtta caaatattct aaatacagta agtacaagta taaatctaag
    45181 aaaaagagaa cttgcagtaa tacaatctat aggggttact cctaaaggat ttaggaaaat
    45241 gatatattta gaaagtttta tatatggtat actgtcattg ttgtttggaa taccaattag
    45301 tattggtata attctaatta tgaataaact tatttcaggt gtaattgaat tttctccgat
    45361 tataccgtgg acagccatag ttatctgtat agtaagtgtt tttataataa cttttatagc
    45421 agggtatata ccaatgagta aattaaataa agaaaatata atagataata taagaagaga
    45481 aagtatatag atataaaaat aattcttaac tatataaaat attattatat cacttaaatg
    45541 attttttatt tatataagaa atctaataat aaattactag aggtatttaa ctatttagaa
    45601 agaattggta ttatataaag taagagaata tataataaga aagaatattt gattttattg
    45661 gaaaacaaaa gaaaaagaag gtgattacta tttctcatat aactgcgaag aatatgtata
    45721 aaagtttaga ggaaagaata aacaagtttc cacaaggtgc tccaccatct gatacattat
    45781 acaaaatact aaatgtacta tatacagaac aagaagcgaa gttagttgct cagcttccaa
    45841 taaagccttt cagagtaaaa acagcagcta aaatttggag tgtaagtgaa agtgaagcat
    45901 atagagtatt ggataaactt gctagtaagg ctctgatact agatatagaa gataataaag
    45961 ggaagaaata tataatgcct cctccaatgg ctggtttttt tgagtttgcc atgatgagaa
    46021 cgagacacga tatagaccaa aaacttttgg cagagcttta ctatcaatat atgaatgtag
    46081 aagaagattt tatcaaagat ttattttatt caacagaaac gaagcttgga agagtatacg
    46141 ttcaagagga agttttaact aatgataatg aggttagtat tttagattat gaaagggcta
    46201 ctcatataat tgatgaatca actcatatag gtataagtat gtgttactgt agacatagaa
    46261 tgcaacatgt tggaaaagct tgtgatgctc ctatggatat atgtatgacg tttgacaatg
    46321 tagcaaattc tttaataaat aataaatttg caagacgtgt ggataaaata gaatgtaaag
    46381 aactacttca tcaagcatat gaacataact tagttcaatg tggagaaaat gttaggaaag
    46441 gggttacgtt tatatgtaat tgctgtggat gttgctgtga agcaatggta gcagctaaaa
    46501 gatttggaaa tcttcatcca gtacagacaa caagttttat accaaatata aaccatgaaa
    46561 attgtgtaaa gtgtggtaaa tgtataactg catgtccaat agatgcgata agcaaggtta
    46621 aggaagatgg taaagaatat ataaaaattg atgaggatag atgtctaggt tgtggagttt
    46681 gtgttagaaa ttgccataaa aatagcatta tgttattaaa gagagatgaa aaaataataa
    46741 ctccagcaaa ttcagtacat agagcagttc ttatggcaat tgaaaaaggg cagttacaaa
    46801 atttaatttt tgataataat gctctggcta gtcatagagc tatgggagct atactgtcag
    46861 ctatattaaa gcttgaacca gctaaaaaga ttttagccag taaacaatta aagtcagtat
    46921 atttagataa attgttaagt atgaatgata aatagaaact aacttaaaaa aggcaaagtt
    46981 ttagacttat ctatatctta ataaacttat catttaatat ttaaatttaa tttttacaca
    47041 tataaaactt ataacttaaa taaaaaaagg ctatattcaa ttaatattac ttgaatacag
    47101 ccttttttag ttatgttaag ctatttcttt tatttgctta aatgagtttt catttccatt
    47161 tccaccaatt tgtgttacag atttaaatct catagtttta aatagttctt tttgattata
    47221 atctagtgag tttcctacta gcattacagg agatttagtt tttccagcca aaactccaac
    47281 agataatccg tctattaaat catcttgttt attcattcca tttttagcaa catataaatt
    47341 gtctatctta gaatccttgt aaaattcttt tattactttt gcattagttt catttctagt
    47401 gctacctgat attctctgtg gattttgaag tttactttct atatttttag aaactgtata
    47461 ttctccaccg attacataag atttatcaat ctttttgttt ttaaacaagt tatttatttc
    47521 agttatatta ctattttcat tagtaagtat tataggcata tcattttgag ctgatacagc
    47581 tccaatgctt actgcatcgg ctaatccttt ttcgccatta actactgaaa tctttgatat
    47641 attgctaata ttgttcaatt ctttagcaat gttcatagaa gtttgatatc tatcacttcc
    47701 tgaaattcta gatacagaga tattgtttga ttttatagta tctaaagatt tttcatttat
    47761 agaagcttct ccaccaacta catatacatt tttaactttt aatctcttta attcagccaa
    47821 agttttttca tttatttgag agcttccagt caataatata ggagcgtttt tcttgtatgc
    47881 aaatggtgtt gcagccaaag catctgcaat tgctgaatcg tttattaaaa ctgcattttc
    47941 agcattttcc caacctgctt gacttatctt aatagaagtt tcatatctat cagttcctgt
    48001 aagtgcactt gcatttactt catcttgata tgtaacctca aatctagcat ttggacttcc
    48061 taagaaaaca attacgcctc cttttggcaa tctaactcta ttgttctttg taactaaaga
    48121 atagtttaca ggaacacctt tatcatcata aacagcaaaa gctgaatttt gaggaagatt
    48181 aacttctatt tttttatttg cgatatcgtc accaatctta taccattttg cataaccatt
    48241 ggattctagt tcacaagtaa aagatttttc tgctggcaaa ttagttatag aatcttcact
    48301 aacatacgtt agtgttccaa aagataaata ctctgtacca ttttctttat gaagtttaat
    48361 gtcggataaa tcacgaccta ttacaccagg tatttctata aaagcatttg agttattctc
    48421 atccattatt ttagtgttaa ctatataacc tggagtttca tcagaaagac taaaacttgc
    48481 ttttacagag ccaaacatat agctttgaga agtatatttt tcatccacta gatagtaacc
    48541 tttgccactt ctttttttcc acacttcttt gacactattt gatatattgt tgtcatctac
    48601 tttttgagct acatagtata aacttgctgt ctgacctaat ccagggacat catcataaga
    48661 gctcatattt aaatatgtga tattattttt ttcttttaca aattttaaac aactattacc
    48721 tttttcagat acaaatctat cttgacctat gtatacgtat ttatcttcag gtccattttc
    48781 tatataagga gaagatactg ttaaagtacc attatcatta acatctactt ttatcatatt
    48841 tgaagaagca taaagaccac tattttcttt taaagagcta ggcattttaa cttgttgtgg
    48901 cttactaaat gttttatcag gttttatttc tttaatttta cctttttctt ttaatgcaga
    48961 tagtaagatt tcttgtccaa ttatttcatt aagttgacta cttccaccag aagaaagaac
    49021 tgcaactgcc atattttcat ctggaagaac tataagatta ctgtggaata ataaagaatc
    49081 tccacctttt gtaagagctt ttaaattata ctgattaaat ggataagtgt tgacacaatc
    49141 ccaccctaaa ccatatccaa gtatagagtc ttctccttca ggccatagac catttaaata
    49201 ttctttgttt tccatagctt ttactgaagc aggagaaaga ataccatttg aatttttcat
    49261 aaatgtttgt gcaaatgtac atagattttc tgcacttgaa tataaaccac ctgcacctat
    49321 agcattaagg ttatcttgtg gaactgcatc ttcccaataa ggtacatatg cttttgctag
    49381 ttttgaacta tcaaaactat tttctggagt ttttgtattt tgtaagttaa gtggattatt
    49441 tatgtattta tcaagaaaat tagtaaatga cataccagat actctttcta ctaaaatttc
    49501 agctaaagta aatccatcat tacaataaac agaaaaagct cctggtttag cttttaatct
    49561 ttgtttttgt aattctttta agaaattatc atgaccataa gaatcattgt ctgctaataa
    49621 aatagtgttt ttaaaactac ttcccatcaa ccctgatgag tgatttaata acattcttgg
    49681 agtgatttcc ttgtatctat catcagccat tttaaattca ggtatatact ttacaacagg
    49741 agtatctaaa tttaattttc catcatcaac caacttcata acagctgttg tagtaaacat
    49801 tttactgata gatgcgatac tatacatatt atccttattt aagtttttgt tatcctgttt
    49861 gctgtaaact ccaccattac cagaaatctc aattttgcca ttatcaatca aagcatattg
    49921 agcacttaca gcaccatact ttgatagcaa agtttggatt ttctctttag caagagcttt
    49981 agtttttgtg taagattccg cgtttttact ataatcatgt aattctatat cagaactttg
    50041 atatttttcg gaattatctt ttgtattatc agcaaaagta atatttggag cagcagtaaa
    50101 taacatactc aatactaatg tactagataa taatttagag agcctttgct taaacatatt
    50161 acattacctc tttccctttt taataattta taattagcat aacattctta attattttaa
    50221 tcaacagatt attatcaata aaaaaagctt ttataagtat agctttttat ataacagaga
    50281 aaaatataaa aataatattt gttgttgtat aaagaataaa gggtataggt aatatataaa
    50341 taaaatggag gaattttaga catatgtata agaatataaa taagaagcaa cttaaagaaa
    50401 tgatgaaaaa tgaaaaggat gttttattat tagatgtaag aactaaagat gaatttaagg
    50461 aatataaaat agaaaatgct ataaatatat ctttacaaga gttaatcaat aatatagatg
    50521 aaatttacga ttataaagat aagaaagtag tagtttattg cagaagtggt catagaagtg
    50581 ttactgcatg taatttgtta gctgaagaag gttttgagca tttatataat ttaaattcag
    50641 gaataattga ttatatgagt taaataatgt aagttataca ttaaatacta tgtatagctc
    50701 tgcttattaa gtaggaatat atctaaatat ttataagata aatttaggaa agaacgaatt
    50761 ttttcgtttt tttctatgac agtttttgta gtaaatatta gtttttaagt tggaaaaagg
    50821 tagtagcttt ttagctacta ccttttaata tgaacttaga atattattaa aaaaatatat
    50881 ttaaagacag aattaatttt ttaatgacca gttaaattac tatcgccgaa tttatcatta
    50941 aatggtttta taactctatt tataggtcct tttagaagta catttaaaca aatagacaga
    51001 atcataaata gtaatagaac tagtatatct ttagttagat ttggagcata tataccacca
    51061 actgcttccc ttgtagcacc tattgcatag gtaaatggca ttatagggtt tattctttgg
    51121 aagaatgtag gtgtaacttg tatagggaat gtaccaccag aagaaccaat ctgaacgact
    51181 aatagtacta ttgcaattgc ttttcctaca tttccaaata cagaaactaa agaatacact
    51241 ataaagttaa ataccatact agtaaacata agcagaacta tcaataaaat tggatgtttt
    51301 gctgttactc ctaacaataa tatatctcca gttcctatta taaaaccttg gacaagagct
    51361 atacttaaaa aagtaaggcc tcttccaaag taaatttcat aaggtttata gtttccatgt
    51421 acagatgtag acaatagtgc tgaaataagt aatataccaa cccatgtaga aagaacacta
    51481 taaaatggtg tcattgcaga accataattt tcaattggat ataatttctc tgttttcacc
    51541 tcaacaggtt cttttaagaa attagacttt gtcaatatat cactttcaag caagctgact
    51601 aattttttca tatcttcacc attactaatt ttgcttaaag tatcaatcaa atcatccaac
    51661 attccttttg ctaaaggaag tctttctctt atcaatgata tactttcctg tgcatttcct
    51721 gaaagtttta gagaagtagt aagtatttct tcaacctttg gaagttttgc ttctgcctta
    51781 tctaaaacag taattatatc atttgctact tttatactgt ttgcaaatat actatttata
    51841 ggttttgata tttttgagtc aaaattattt aaaatattta aattaattct tccaatacca
    51901 tttgatagac ttaaaacatt attcaaagca cttatagatg gttgttgtcc agatataact
    51961 ttgtttttaa tatcatttaa tgttgatata gatgaatcta ttttattgct agagtcttca
    52021 agattatcta tgacatcatc caatctatta tttgatgtaa gttggtttaa tttagttaaa
    52081 aaatccacca aagtatcatt taaagattgc aaattagaaa gtttttctga taaattatct
    52141 atcaacttag gtacatcttc actgccacta tttactgcat caattaaatt taatgtaaga
    52201 gaagaagcag aagatgaaag gtcaaccatt aaatttaagt cagacttaat aagaggagaa
    52261 agttcgtcta aattatcatt tgtatcctct aagaattttt ttaagtcact tgataatagt
    52321 tttgtatcat tcagagtctt tttaattgta ggtaaatcag atttaatatc tttaactgta
    52381 tcagataatt ttgaagtagc atcagaagct aaatcaacag tcttatttat atggtcaaat
    52441 ctcttttgaa ggtcaataag ggaattttca acttttgtca attttggaag actgtgttca
    52501 attcctgcac caactccgtt tgatatacca agaacgattt cactaactgt ttttactata
    52561 gtctgattaa cctgtagttg tattgtagaa gcacctttat ctgtaatctt tggtgcaact
    52621 gcatttattt tttcattaac agtatagata atttcgcctt ttttaacatc atctgtaatt
    52681 aaagatgtca aatcttttgt aaaatttttt ggaatcttaa gtgatgcata ataagtacct
    52741 tttttaacac cttcaagagc agttttttca tctacaaact gccaacctaa actcttgtta
    52801 tctttgagtt gttcaattaa ttggtctcct acattaatct tttgtcctgc tatttcattt
    52861 cctttatctt catttgttac tgctacaagc atattagcag tgcttccata agggtcccaa
    52921 gatgccttta tattgaacca agcatacaga gatggtaaaa aagcaagacc taaaactatt
    52981 acaagtacgg cataattgcc acgtatatct tttaggtcac ttttaaaaat ttctaatata
    53041 ttttttaaac cctttgaagg tcttaaattc ttgatattat tccagtttaa aatttctttt
    53101 aaactattag tttttttcaa atgtctcacc tcctaaaata ttttgataaa gaaaaaagtt
    53161 agatttataa tcaattttaa attatattac tttttacaca aatagtaaag ttaaaatttt
    53221 gtc
//
LOCUS       DAMJWS010000017        51562 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMJWS010000017 DAMJWS010000000
VERSION     DAMJWS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.017"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            154..3700
                     /locus_tag="PC399_001084"
                     /pseudo
     CDS             154..3700
                     /locus_tag="PC399_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            3885..5819
                     /locus_tag="PC399_001085"
     CDS             3885..5819
                     /locus_tag="PC399_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF2591958.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5925..6920
                     /locus_tag="PC399_001086"
     CDS             5925..6920
                     /locus_tag="PC399_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2591959.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6892..7677
                     /locus_tag="PC399_001087"
     CDS             6892..7677
                     /locus_tag="PC399_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2591960.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7665..8363
                     /locus_tag="PC399_001088"
     CDS             7665..8363
                     /locus_tag="PC399_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591961.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8486..8959
                     /gene="ybaK"
                     /locus_tag="PC399_001089"
     CDS             8486..8959
                     /gene="ybaK"
                     /locus_tag="PC399_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF2591962.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9156..9995
                     /locus_tag="PC399_001090"
     CDS             9156..9995
                     /locus_tag="PC399_001090"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF2591963.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10008..10556
                     /locus_tag="PC399_001091"
     CDS             10008..10556
                     /locus_tag="PC399_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF2591964.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10622..11803
                     /locus_tag="PC399_001092"
     CDS             10622..11803
                     /locus_tag="PC399_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF2591965.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPAPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            11971..12357
                     /locus_tag="PC399_001093"
     CDS             11971..12357
                     /locus_tag="PC399_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2591966.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDTDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12507..12770
                     /locus_tag="PC399_001094"
     CDS             12507..12770
                     /locus_tag="PC399_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2591967.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            13296..13979
                     /locus_tag="PC399_001095"
     CDS             13296..13979
                     /locus_tag="PC399_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591968.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGKFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13990..15231
                     /locus_tag="PC399_001096"
     CDS             13990..15231
                     /locus_tag="PC399_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2591969.1"
                     /translation="MKIFSNKDIKIFFLMLISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIILNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISVNGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSIFTISFLKLTNM"
     gene            15303..15986
                     /locus_tag="PC399_001097"
     CDS             15303..15986
                     /locus_tag="PC399_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591970.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15983..18313
                     /locus_tag="PC399_001098"
     CDS             15983..18313
                     /locus_tag="PC399_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591971.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDNGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANIGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNGNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAVSGCLLGSILGLILHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPIKKICELDIIDSINAQ"
     gene            complement(18494..19336)
                     /locus_tag="PC399_001099"
     CDS             complement(18494..19336)
                     /locus_tag="PC399_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2591972.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            19631..20365
                     /locus_tag="PC399_001100"
     CDS             19631..20365
                     /locus_tag="PC399_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2591973.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGRPIEYTISIFRTDKYQYEIVLSE"
     gene            20375..21508
                     /gene="nagA"
                     /locus_tag="PC399_001101"
     CDS             20375..21508
                     /gene="nagA"
                     /locus_tag="PC399_001101"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2591974.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLNNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            21607..22356
                     /gene="nagB"
                     /locus_tag="PC399_001102"
     CDS             21607..22356
                     /gene="nagB"
                     /locus_tag="PC399_001102"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2591975.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            22508..22780
                     /locus_tag="PC399_001103"
     CDS             22508..22780
                     /locus_tag="PC399_001103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591976.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            23440..24144
                     /locus_tag="PC399_001104"
     CDS             23440..24144
                     /locus_tag="PC399_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2591977.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAISRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            24146..25633
                     /locus_tag="PC399_001105"
     CDS             24146..25633
                     /locus_tag="PC399_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2591978.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            25838..27451
                     /locus_tag="PC399_001106"
     CDS             25838..27451
                     /locus_tag="PC399_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2591979.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(27814..28644)
                     /locus_tag="PC399_001107"
     CDS             complement(27814..28644)
                     /locus_tag="PC399_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2591980.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            28717..30444
                     /locus_tag="PC399_001108"
     CDS             28717..30444
                     /locus_tag="PC399_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591981.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSHFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            30481..32343
                     /locus_tag="PC399_001109"
     CDS             30481..32343
                     /locus_tag="PC399_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591982.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            32676..33365
                     /locus_tag="PC399_001110"
     CDS             32676..33365
                     /locus_tag="PC399_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF2591983.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYQIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            33470..34156
                     /locus_tag="PC399_001111"
     CDS             33470..34156
                     /locus_tag="PC399_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF2591984.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            34173..36080
                     /locus_tag="PC399_001112"
     CDS             34173..36080
                     /locus_tag="PC399_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF2591985.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPIEVGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            36531..37220
                     /locus_tag="PC399_001113"
     CDS             36531..37220
                     /locus_tag="PC399_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2591986.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            37656..38195
                     /locus_tag="PC399_001114"
     CDS             37656..38195
                     /locus_tag="PC399_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2591987.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            38214..39257
                     /locus_tag="PC399_001115"
     CDS             38214..39257
                     /locus_tag="PC399_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2591988.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            39259..40095
                     /locus_tag="PC399_001116"
     CDS             39259..40095
                     /locus_tag="PC399_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591989.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            40200..40874
                     /locus_tag="PC399_001117"
     CDS             40200..40874
                     /locus_tag="PC399_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2591990.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            40874..41926
                     /locus_tag="PC399_001118"
     CDS             40874..41926
                     /locus_tag="PC399_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2591991.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            42156..43004
                     /locus_tag="PC399_001119"
     CDS             42156..43004
                     /locus_tag="PC399_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2591992.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            42994..44658
                     /locus_tag="PC399_001120"
     CDS             42994..44658
                     /locus_tag="PC399_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2591993.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLNKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            44707..45963
                     /locus_tag="PC399_001121"
     CDS             44707..45963
                     /locus_tag="PC399_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2591994.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            45986..48067
                     /locus_tag="PC399_001122"
     CDS             45986..48067
                     /locus_tag="PC399_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF2591995.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSMKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            48104..49189
                     /locus_tag="PC399_001123"
     CDS             48104..49189
                     /locus_tag="PC399_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2591996.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGFVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            49243..50367
                     /gene="wecB"
                     /locus_tag="PC399_001124"
     CDS             49243..50367
                     /gene="wecB"
                     /locus_tag="PC399_001124"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF2591997.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGMQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            50445..51419
                     /locus_tag="PC399_001125"
     CDS             50445..51419
                     /locus_tag="PC399_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2591998.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT        20547 a         5247 c         8912 g        16856 t
ORIGIN      
        1 ttacaaataa atcttaagct atcttattta attctaatac aggaagaagg taaatgtata
       61 ttgaaaaaat catagaaata gaagtaaaca atttaaataa atacaaaggt ttgcaaaaga
      121 agtaatataa ttatatacaa tgaggatgtg agtatgatga acagactaaa gccaagaatt
      181 ttaggtatta gtatcttgtt tattattata attattttaa catgtggagc tgttttcaag
      241 tttaaattta aattagataa taaattaaaa gaaaccacac atacatattt gtctgagttt
      301 acagaacaca atataattaa tctcaagaca agacttagtg gtcaatttga catgcttgaa
      361 tctattgcta acttcatagg aaacttgaat aatataaatg atgaaatggt gattgattta
      421 atggattctg atgtaaagcg aagttcttta attagaatga ctatctctac attagatggg
      481 gaatgttatt ctaatgatgg aattacccat aatgttcaag atagagatta ttttaaaaaa
      541 gctaaaaatg gagaaaagaa tatatcagag cctttaaaat ctattgttaa taataaagaa
      601 ataatagttt tatctacccc agtttataaa agtgggaaaa tagttggtgt tttaagtgga
      661 atttatgatt caaaaaagtt aaatgaatta ttaggattat ctgcatttga tgaaaaagca
      721 gatgtttttg ttgcaaagaa aaatggagat ataattgctc aaaaaacaaa taaaaatagt
      781 aagacgatat catgtatatt tcagcttttt gacaaagata attgtgatga agttgattgt
      841 ctaaaaacta taaaagaaaa tatgaaaaat gataagtctg agtattttaa ttataactta
      901 gataatgaaa aatttatgtt aaactatcag ccacttggag ttaatgattg gtatgttttt
      961 tctgttgtgc caaataaaat agtgtctatt cagtcagaga atatatctta tgatgtatat
     1021 atttttgcat gaaatactac tagttatatt aatttttata atatatatag catgtattat
     1081 aaataataac accaaaatta ttataaatga aaaagaaaaa tttaaagtac tcactgataa
     1141 tatactagga ggggttaaaa cctgtttaaa agatgaatat atgactatga catatgtaag
     1201 tcaggggttt ttagagctta caggttactc ttgtgaagat ttaaataaaa tatttaaaaa
     1261 tagatttaaa gacatgattt ataaagatga tttagacaag tttaattgta ttattctaaa
     1321 ccaattagaa aaacaagata aaattgaagt tgaatataga attgtaagaa aagatggaag
     1381 tataatttgg attttggaga gaagtaagtc tataaatgat aataaaggaa gtgagtgtat
     1441 tcagggagtt ttaactgata taacagaatt taagaaaata caagaagagt tagattcaaa
     1501 aagatttgaa atacaggcta tcaatgatag tattgttggt ggaatagtga taactgagat
     1561 aaatgatgat tttgatagta tatatttaaa tgaaggttat ttaaatatga ttggatatac
     1621 acgcaaacaa ctgaaagatg agctaaataa taaattaatc aaacttattt ttaaagatga
     1681 tagaacagaa ttaattaata gtataaacaa acaactcaaa ttaagtgata catttgcatc
     1741 tgaacaaaga gttgaaaaaa gggatggcac tattatatgg attttattaa aaggaagaaa
     1801 gatgatttat gataatggaa aatctgtact ttgttgtgtt attgtagata ttacttctgc
     1861 taaattatta gagcaagaac ttaaaaagag tgaagaacgt catgaaatca ttctaaagca
     1921 gacgcaaaat attataatag aatgggatat agaaaaagat aaaatgaata tatcacctaa
     1981 ttggtataat aagtttaaat atgaccctat aactgaaaac gcaacaaaaa atataattaa
     2041 atcagtagat atatatcatg atgatatgga taagtttatt tcattaatta aacttacttc
     2101 acaaggtagt gagtatgaag agtgtgaaat tagacttaga actagagata ctatttacgc
     2161 ttggtttagt ataaaggcaa caactttatt tgataataat ggtaaacctt ataaagtagt
     2221 aggaagtttg actgatattg atgaatcaaa acgagaagct gaaaagttga aagaaagggc
     2281 agaaaaagac cctcttacag atttgtataa taaaaaagca tcacaatcac ttatagaaga
     2341 gtacataaca ttggataaaa aaagaaaagg tgctttaatg attattgata ttgatgactt
     2401 taaaggaata aatgataatt taggacattt atatggggat gctgtattaa gtgaagttgc
     2461 aaatgaccta ataaatctat ttagagcaac cgatatagta ggcagaattg gtggagatga
     2521 gtttattgta tttataaaag atatttctaa aacaagagat attataaata aagcagaaga
     2581 attggtaaaa acatttgaaa ggtctttttt aggtaaagga cgaaattata aaatatcatt
     2641 aagtgtagga attgctaagt ttccacaaga tggtatggac tatatgacac tatttagaaa
     2701 tgcggatata gctctttact cagccaaagg gaagggcaag aattgttata cattgtatga
     2761 taaaaaaatt gataaagtcc aatatataac taatacaaga aatgcaggag atagtgataa
     2821 tgtagagatg tcttttaaag aacatatcac aaattatata tttgatatac tatatgaatc
     2881 aagtgatata gaagtggcag taaatttaat tctaagtatt gtaggtaaac actttaatgt
     2941 gagtcgcatg tatatatttg aaaattcaga tgataatcga gtatgtaaaa atacatttga
     3001 atggtgtaac gaaggtataa cctcggaaat taataatctt aaaaatattt gttacaagga
     3061 tttaggcaac tatcttttaa attttaatgc agatgggtta ttttattgta atgatattga
     3121 aaatttaggt agtgatgtga aaaaagtact taagccacaa ggcatacgtt caatattaca
     3181 atgtaaaatt ttagagggta atgaatttag agggttcata ggatttgatg aatgttcata
     3241 taatcgtatg tggactaatg atgaagtgaa cactttgaca tttatatcta aaacgctcaa
     3301 tatgttttta ttaaaaatgc gtattcagag taagctagaa gagtctttaa caattcaaaa
     3361 atctgtgatg gataatatgg atatatgggc atatgttgtt gacaggaata cctatgaatt
     3421 actatttatc aataaaaaaa tgcttgagtc agcaccaaac actcatgtag gtgatttatg
     3481 ttataaagcg atatggaata atcaagataa accttgtaaa aaatgcccta tgatatattt
     3541 aaaagatggt gatgatactt ataaatcatt catgcataat aaaaagtttg aggtacaggc
     3601 aaatataact acaactactt tgaaatggtt tgatggtaaa gatgcatgtt taatatttgg
     3661 aagtgaaata actagtattg aagctgaaga aaatacttaa aatactgtgt tatatatata
     3721 cttaaaatcg caatttgaat ttattagact taaaatgtct atataaatca tttaagttgt
     3781 ggttttaagt attttttatg atataattaa gcgttaagaa caaaattaaa agatactttt
     3841 gtataaaaaa atatttgtta aatatatatt gagcaggtga taatatggaa aataagtttt
     3901 taccaataag caaacaagat atgatagata gaggatggga agaattagat tttgttctag
     3961 ttacaggaga tgcttatgtg gaccatcata gttttggaac agcaataata tctagagttt
     4021 tagaaagtgc tgggtataaa gttggaataa tagctcaacc agactggaga actacagatg
     4081 attttatgaa gctaggaaaa cctagacttg catttctagt taatgcagga aatatggatt
     4141 caatggtcaa tcattattct gtaagtaaaa agcatagaga aaaggatatg tattctccag
     4201 gtgggaaaat gggttataga ccagatagag ctactatagt atattgcaat aaaataagag
     4261 aagcatacag tgatgttgca atagtaatag gtggaattga agcgagctta agaagatttg
     4321 cacactatga ttattggagt gataaggtta gaaagtctat gttaatagat agtggagcag
     4381 atttattagt ttatggtatg agtgaaaagc agattgtaga agtagccaat gcacttaatg
     4441 atggatatga cccaaaatac attagacata ttgatgggac atgctatatt tcagatacat
     4501 tggaagaaat atatgataaa tatatattaa ttccatcata taaagaaatt tgtgaagata
     4561 aaatgaagta tgtagaagct tttaaaattc aatatgatga gcaagaccca tttagaggta
     4621 atataattgt tcaacctcat ggaagtaagt atctggttca aaataagcca gaaaaaccac
     4681 ttagtagaga agaactagat gaagtatatg gattaccata tcagaagaca taccatcctg
     4741 tgtatgaaaa atttggagga ataccagcta tagaagaagt aaaatttagt atagtaagtt
     4801 caagaggctg ttttggtagt tgttcatttt gtgcaataac atttcatcaa ggtagagcag
     4861 tgcaaagtag aagtgaaaaa tcaattattg atgaagcaat aggaataaca aatttagatg
     4921 attttaaagg gtatatacat gatgttggag gtccaacagc taattttaga agaccagcat
     4981 gtaaaaaaca aattactaaa ggtgcttgta aaaatagaca atgcttatct ccatcaccat
     5041 gtaaaaattt agatgcagac cacagtgaat acttacattt acttagagct gtaagaaaat
     5101 tacctaaaat aaaaaaggtg tttgtgcgtt ctggaattag atacgattat gtaatggcag
     5161 ataagaacaa taaatttctt agagagttga tagagcatca tgtaagtggt cagttaaagg
     5221 tagccccaga acatatatca gaagaagttt taaagtatat gcaaaaacca gcaggaaaaa
     5281 cttatgataa atttagacag aaattttttg ctataaatga agaattaggt aaaaagcagt
     5341 atttgatacc ctatttaatg tctagtcacc ctggttctac cttaaatagc gctatagagt
     5401 tagcagagta tttaagagat actcactacc aaccagagca ggttcaggat ttttatccaa
     5461 ctccagggac tctatctaca accatgtttt atacaggtat tgacccgctt acaatgaagc
     5521 cagtttatgt tcctaaatca aaacgtgaca aggcaatgca aagggcttta ttacaatata
     5581 gagcacctag aaattatgat ttggtatact ctgcgttagt tgaagcagga agagaagact
     5641 taattggttt tggacataga tgtcttataa agccaaaaaa tgaaaagcct tattttaata
     5701 gaaataattc taaaaaaaat gtatctaaag gaacaaataa aaacaaaaaa actaatacaa
     5761 ataacagaag taataaaaat caacaaaaaa gttcaactaa aaaaaagaaa aaacattaaa
     5821 gtataaataa aaatacttaa aaaagaagga ttattcgata aattaacaga aattagtata
     5881 aataaatata attattttaa tgacgttacc tttgggagga agagatgaaa aagtatattt
     5941 ctataatatt attagtggtg ttgactatgg ttgttttggt aggatgttca ccaggaaaag
     6001 ataaccctaa aaataaggag ctatcagttg tcaaacaagt aaaagtagct gttccagatg
     6061 gactaccagc agtggcaata gctaaacttg caaatgaaaa tccagagatt aaagaaggat
     6121 atgaaacagt gtatagcatt gaaaagacac ctgaagcgat ttccactaga gtgatgaaaa
     6181 aagatgcaga tatagctata gttccttcaa acatggctgc aatagcgtat aataaaactt
     6241 catcatataa tattgttgga actgtaggaa tgggttcact ttatttggtg tcaacagaaa
     6301 atataaaaga ttactctgat ttagttggta aagaagttgg atgtactgga aaaggactta
     6361 ctccagacat aactataaaa agtcttttgt tacaaaagga tataaattat tcagatataa
     6421 aatttaatta tgtaaattca gcaagtgaac tggtaccatt attggctact ggtaaagtta
     6481 aaacaggtat tgttccagaa cctgcactta gtgctttgat gtctaaaaat ccagacataa
     6541 aaattataaa aagtttaaat gactcatgga aggaagtaag tggttcaaaa gatggttatc
     6601 ctcaatctac tttaattgta aagtctgaat ttttaagaga taataaagat tttgttgatt
     6661 cttttgtagg tcaattatca aatagtatag attgggcaaa caaaaatcct gaagaattag
     6721 gtgcatattc tgaaaaaata aaaatatcta ctgaatcaaa gataatagga aaatctcttg
     6781 aaagagctaa tttgaaatat attccagtta aggacatgat taaagattat aaaaattact
     6841 atgagaaatt agctaatttt gatgataaga cattaggagg caaagtacca gatgaagcaa
     6901 tatactttgt tgagaaataa aaaaaataaa gaacaattta tagataaaat ggaggtaaca
     6961 ctttcttgtg ttatcctctt atttttatgg caaattattg ctttaaaaat tgataatgat
     7021 atttttttac caacagtagg tcaagtgttt aatagtataa aagagattat gctaaattct
     7081 agtttctata tagatatact ttactctatg gggagatgtt tttttagttt tttactggct
     7141 atgatttttg ctattatact ttcaatagtg tcttatttaa atagattttt tagaaactta
     7201 ttaaagccaa tcaatgcttt gacacgttca ataccaacta tgatattggt agtacttgca
     7261 cttatttggt ttgaaaagga tagtacccct tttatagttg gatttactat agtttttcct
     7321 atattgtatg ataacgtttt gggcgctata ttgaatatag acaagaactt attagaaatg
     7381 gctaatgtgt ataaaataag gtttatagat aaggtgttaa aaatttattt accagccata
     7441 aaatttcaaa ttatatctat actagtatca acattctctt taagtttgaa agttgctata
     7501 gcaggagagg tatatagtca acctacatat ggtattggaa ctatgataca gactgaaaaa
     7561 attaacttta acacatctgg aatatttgca tggataatta ttgttctttt aatatctgct
     7621 attttacaaa tagctcaaaa gtttttagca aggagagctt ttttatggaa aagataaagt
     7681 tgagtattga aaatataaat aaaagatatg atagtcgtat tatatttaga gattttaata
     7741 ttgattttta tgtaaatgag gtaaattgta tccttggaaa atctggttgt ggaaaaacta
     7801 cactcttgaa cataataagt gggattatta aaaatgacac caacaatcta aatatcaaag
     7861 aaaatttaaa tagagtagga aataagcttg aagctagtta catatttcaa gatgatagac
     7921 ttatagactg gttaacagta gaagaaaata taaagatagt tgtaaataaa tactataata
     7981 aaactcaatt aaataaaatt tgtgatgaat acttggaatt ggttggaatt agtgattata
     8041 aaaaattcta tcctcaaatg cttagtggag gtattagaca aagagtaaat attgctagag
     8101 cttttatata tccatcaaaa aacattataa tggatgaacc ttttaaatct attgatgcaa
     8161 agaatacaca gcttataatg gataatttca gaaatatatt aagaaaagaa aagagaactg
     8221 tgttatttgt aactcataat attgaggaag cattgttttt agcagatagg atatttattt
     8281 taggagactc tccaattaga ataaaaaaaa tattaaaaaa ttctaaggaa ttggaaaaaa
     8341 atgaagtttt gaagttgata tagagttata ataatactgt cgttttaaaa tatatttggt
     8401 taaaatctat aagactatat taaaatagta cagaatattc aaaactgtgt ataatgttcg
     8461 tattttataa aaaggaagtg aatgtatggt taagactaat gctatgagaa tattagattc
     8521 aaataagatt gattataaag taatgtctta tgaagtgaaa agtgaacatg tggatggtgt
     8581 tgaggtggca catgatatag gaagagatgt aaatgaagtg tataagacat tagtaactca
     8641 aggagttagt aagaatatat atgtatatgt aataccagta catgaaaact tagacttaaa
     8701 aaaggctgca aaagtagcta aagagaaaag tgtagagatg attcatgtaa aagatataaa
     8761 taagcttaca ggttatatta gaggcggatg ttctcctata ggtatgaaaa agctttataa
     8821 aacttttgta aatgaaagtg caaagaattt agatacaatt attgtgagtg caggaaaaat
     8881 aggatatcaa atagaactat ctccatttga tttacaaaga cttataaaag tagaatttgt
     8941 agatgtaata aaaaaataag aattacatat aaaatataga gcttatattc tcaataatag
     9001 gtgttgtaat attaataaaa tgaataataa ttttaaaata aatattcagt tacttatgtg
     9061 taattggtat aaaaatattg agaaatttta aaaacaatgg taaagtgtaa gtataatcac
     9121 gaatattttt tataaagtaa tggagggatg ttcgcttgag aaaaataaaa tctgaacaaa
     9181 tcgtcgagca ggtaaaaaaa ctttgtatag aagcgagcct atatttagga gaagatgttt
     9241 taagttgtat aaaagaaaaa gcgaaaagcg aaaagagtga agttggaaaa aatatattga
     9301 atatattagt tgaaaatgct gaaatagcta aagaaaaaaa tataccaata tgccaagata
     9361 ctggtatggc tgtatttttt gttgaaatag gacaagaagt tttaattgaa ggagacacac
     9421 ttactgatgc tataaatgag ggagtaagac aagggtatga agaaggctac ttaagaaaat
     9481 ctgttgtaag tccaattaac agagtaaata caaaagataa tacacctgct gttattcatt
     9541 atgatatggt aaaaggtgat aaaattaaaa tagaatttgc agctaaggga tttggaagcg
     9601 aaaatatgag taagatgaaa atgttaaagc catcagatgg attggaagga attaaaaaat
     9661 tcataataga tacagtttca gaagctggac ctaacccatg ccctcctatg gtaattggag
     9721 ttggtatagg tgggactgtg gacaaatgtg ctcaaatagc taaaaaggca ctttttagag
     9781 aactaggtga atttaataag gacgaaaata tagcaaaact tgaaagtgaa ctattaacag
     9841 ctattaataa acttggtata ggacctcaag gcttaggtgg tactacaaca gctttgggat
     9901 taaacataga gacttttcct acacacatag caggtctacc tgttgttgta aatataaact
     9961 gccatgcatc aagacataag aaggtagtaa tttaggagga aaataaaatg ataaaaataa
    10021 ctacacctgt aaatgaaatt gatatagcaa aattaaattg tggagataca attagtttga
    10081 gtggtatact ttatacagca agagatgctg ctcataaaag attgatagat tgtataaata
    10141 aaggtgaaga attaccattt gacgtatatg gtcaaggaat atattatgta ggacctactc
    10201 ctacaaagcc aggagaagtt ataggtgctg caggtccaac gacgagttat agaatggatg
    10261 atttgactat acctctttta gaaagaggtc ttagattgat gataggcaag ggaaaaagaa
    10321 gtgatgaagt tatagaaggt atgcaaaaat acggagcagt ttatcttgct gcaataggag
    10381 gagctggagc gtatatttca aattcaataa aatcatgtga aattattgca tatgaagatt
    10441 taggagctga agctataaga aagattgtag tagaagacct taagcttaca gtagcaatag
    10501 atagctatgg gaataatata tatgagcaag gtcgagcaat ttatgaatgt aaataatgtt
    10561 tagaaataaa tatgaatatc aaaattaaaa atagttgtgt atattttaag gaggaaaatt
    10621 gatgtctaag gattatgcaa aattggcatt agaaatgcat gaaatcaaca aagggaaagt
    10681 aagtgtagaa agcaaggtag aagttaaaac taaggatgat ttatcaacag catatacacc
    10741 aggggttgca gaaccttgtt taaaaataca tgaaaatcaa gatgatgttt atagatacac
    10801 ttctaaaggg aatctagtag cagtagttag tgatggaagt gctgtacttg gattaggtaa
    10861 cattggtgct gaagcatcta tacctgttat ggaaggtaaa gctatattgt ttaaacaatt
    10921 tgcagatgta gacgcttttc caatatgttt aaagactaat gatgtagatg agatagttaa
    10981 atctgtagag ttgatggaac ctgtatttgg aggtataaat ttggaagaca tatcttctcc
    11041 aagatgtttt gagatagaag agagattaaa aaaatcttta agtatacctg tttttcatga
    11101 tgaccaacat gggacagcaa taatagttgc agcagccata ataaactcaa taaagttaat
    11161 tgaaaataaa aaaatagaag atttagaaat agttataaac ggagcaggag ctgctggtat
    11221 agccatagca aagatactac ttaatatgaa tgttaaaaat ataatcttat gtgatagaag
    11281 tggagcattg gaagctagta tagaaaatct aaactatgtt caaaaagaaa tgttaaaagt
    11341 aacaaatata agaaatgaaa aaggaccttt aaaagatatt ataaaaggaa aagatgtatt
    11401 tattggtgtt tcaggtccag gagctgttac aaaggaaatg gttgaaagta tgtctgagaa
    11461 gccaataata ttggcaatgg caaatcctgc tccagagata atgccagaag aagcaaaatt
    11521 aggtggagca tttataatgg gaacaggtcg ttcagacttc cctaatcaag tcaataatgt
    11581 attggctttt cctggaatat ttagaggagc attagatgtt aaggctaaag agataaatga
    11641 agaaatgaag atagctgctg catatgcaat tgctaataca ataagtgatg aggaaatatg
    11701 cccagagtat atattaccag atgtatttaa taagaatgta gtaaaaaatg ttgctaaggc
    11761 agttaaagaa gcagctataa aaactaaagt aaatagaata taaattttta tatgctcata
    11821 tattcttact tttatataca tatctttatg attaaacgtg ttagaaaacg tagtaaattt
    11881 atactatcag actttacctt tattacttaa aatgatataa tatgaatatt ataaattaat
    11941 aaatttaaat ttattgaggt gcaagagata atggaatata aagttaatga ccaagaactt
    12001 aatgcttcaa tgtttatccc attcgttaac aggatatggc caggagatta tgatacggat
    12061 aaaacacaga ctgcattatc aaaaactttg aatataactg cttatgatga taaagtgctt
    12121 gtaggatgtc tgcgagttct ttcagatggt tattattttg gaacaattac agaattgctt
    12181 gttcttccag agtatcagaa acaaggcatt ggtagtaagc ttttccagct tgctaaagag
    12241 aatacaccaa ctatgctata ttttggtgca cagccaggag tagaagagtt ctatgaaaaa
    12301 aatggatgcc aaagaagtct tcaatcttac ataataaaaa agaaagatag ttattaaagt
    12361 tccagctttc aataatctta aaagtacaat attttagtgg aatcatttca ttttttatta
    12421 ctaatatgac ataaacatgt catattagta ttgatatact tttaatatgg ttaagagcca
    12481 aagaaaaata taaggagtga tttaaaatgg aagaaactaa attttgccaa tgctgtgcaa
    12541 tgccaatggg acaaactgat gaattatatg ggacaaataa agatggaagt aaaagtactg
    12601 attattgtag ttattgtttt aaagatggtg agtttacagc tgatataagt atggaagaga
    12661 tgatagaagt ttgtatccct catatgcttc aaagtaataa ggatatgact gaagatgaag
    12721 caagaacaat gatgaacaat ttctttccaa cattaaaacg ctggaaataa ttttatgcaa
    12781 aattagttat atgcaaaaca tgaaatatgg tgttgagaat aaggggtggt gtgctttata
    12841 gcataccacc tgttttgtta cttgctagtt caggtattct tgtatgctta aatatgttct
    12901 catctctctt tctattttaa attgtaaaaa tctaaaatat gttatttata ttctacttct
    12961 ttatccatgt ctttttctac cacaagtcaa atccaactac aaaatcatca ttttcaattc
    13021 actgtttaat agaattaaca gataaagagt tataaaattt tctatttttt atataaaata
    13081 atacaggtca agctgacttc tagctacttg ggatagtgta tatatttatg atagttatag
    13141 gagtaatacc aatcattaga ctgttgattt gtgtagtctt ttgtaaaaac atttgtttta
    13201 atatattgag tttttgaaaa agtaaaatta taattataat aaattaagtg taataatgta
    13261 ttagaagtaa aaataattaa aatagaggtg attaaatgag tagtatattg ttagttgaag
    13321 atgatttaag cttgatatat ggattagagt tctctataca aaaaagtgga tttagtatag
    13381 acatagcaag gacagtaaaa gaagctatgc aaatgtatga ggaaaaaaat tatgacttat
    13441 tgcttttaga tgtatcccta cctgatggta atggatttca gatttgcgaa aaagtacgag
    13501 aggtttctaa tatacccatt atatttttga ctgcttcaga tgaagaggta aatgtagtga
    13561 tgggtcttga tatgggtgga gacgattata taacaaaacc ttttaaatta aatgaattaa
    13621 tttcaagaat aaaagcatta cttagacgtt ataatgttgc aagtaatgtg aatgaattaa
    13681 gttcaaataa tattactatt aaattacttg aaaatcgtgt ttttaaaggt aaatttgagg
    13741 tggaacttac cacagcagaa tataaattat tgtgcttatt tatgaaaaat aaaaatattg
    13801 tacttactag aaaaaacata ttggacaagt tatgggatgg aaatggtagc tttatagatg
    13861 acaatacatt atcagtatat gtaagacgat tgagaaataa aattgaagat aatcctgaaa
    13921 atcctaagtt tctattgacc gttagaagaa tgggttataa gtggaatgta attaagtgag
    13981 ggtattaata tgaagatatt ttctaataaa gatataaaaa tatttttttt aatgcttata
    14041 tctattttag taatgtttat tatagtaggg caaatagtgg ttataagtat aactaacaat
    14101 tataaaaaaa cgttgataga gcatgattat aatatagctg gatacttata tagtataggt
    14161 gttgataaat ctaaaatacc atcagcattt gttgcacatg aagataaata tattttaaaa
    14221 ggaaaagaaa tactagatac agctggatat ggatttgata tagatgttag ctttacacca
    14281 agtgtaaaat tgtttcaaag taaatatata aaaactatgt ctatctattt attagtattt
    14341 tttactatga ttatttttat actatatatg tattttttta ggcaacaaag taagatagaa
    14401 gaggctagtt ataaaattga tttatttatg aatggaaata taaatgaaag actaaataca
    14461 tatgaagaag gtagcttatc aaaattattt agttcaatag atgttatgtc gacatcttta
    14521 aatacacata tattaaaaga aaaacagagc agagaatttc ttaaaaatac aatttcggat
    14581 atatcgcatc aattaaaaac tccacttaca gctttaataa tgtacaatca gatactacaa
    14641 gaagaaagtc ataatagtga tgtcgttgaa gcttttgtac aaaaaagtca aagtgaacta
    14701 gaacgcattg aaacattgat taagaattta ttaaagatta caaagataga ttcaaaaaca
    14761 ataattctca ataaaaataa cgttaatctt aaaaatttaa ttgtcaaaat tttgtatagc
    14821 tttgaaacta gggcagaaaa agaaggaaag gttatttctg taaatggttc agaaaataca
    14881 ttgttgttct gcgattatga atggatgtca gaggctttaa gtaatcttat aaagaatgca
    14941 ttagataata caaaagagaa tgataaaata acgatagagt ggtttgagac gactataacc
    15001 accattgttt cagttaaaga tactggaaat ggaatccatg ttgaggatat tcatcatata
    15061 tttaaaaggt tttatagaag taagttttca aaagataatc aaggtttagg actgggatta
    15121 ccactagtta aatctattgt ggaacaacat aatggtacta tttctgtaga aagtaaacta
    15181 tcacaaggaa gtatattcac cataagtttt ttaaagctta ctaatatgta agataaaata
    15241 catcttgcag taaggtatat agattattat tatattaatc aattagaaag gtgagagaga
    15301 aaatggatat tcttgtggtt aataatttat gtaaaacata tggaaatgga tatacaaagg
    15361 tagaggcatt gaaagatgta tcctttacag tttcaaaagg agagtttgtg actgtagttg
    15421 gagaatctgg ttcaggaaag agtacactat taaatctctt aggaggtctt gatactccaa
    15481 catcagggag aatatttatt gatggtaaag aaatatgttc aatgaaagag gaaaaacaga
    15541 ctatttttag acgtcgtaac atagggttta tatttcaggc atataacctt ataccagaac
    15601 taactgtgga gcaaaatata atattcccat tgttattaga ttataaaaaa ccagatacta
    15661 tttacttaga tgaagtattg actattttag gtcttaaaga taggaaaaat catcttccaa
    15721 atcaattatc tggtggtcaa caacagagag ttgcaattgg aagggctttg attacaaagc
    15781 caagtattat actggcagat gagccaacgg gaaatcttga cagtaaaaat agtaatgatg
    15841 taatttcact tttaaaagtg actgcagaaa agtataatca aaccattatt atgataactc
    15901 ataatacgaa tatagcttct acagcagata gaatactaca ggtttctgat ggtgtgctta
    15961 cagacctggg ggtgaataga gaatgagaag ctacttagaa ttagtaccac aatatgcaaa
    16021 agtacataag aaaaaaaata gaactacaat tttatgtatt attattgcag tatgcttagt
    16081 tactgctatc tttggacttg cagatatggc tatacaatgt caaaagatac aatttattaa
    16141 agatgatgga aattggcaca tagtaattag aaatattgat gaaaagttta tatcagaaat
    16201 aaatagtaga gtagatatag ctcaccatgg tagggggcaa gtcgttgaca atggagagtt
    16261 taaatcaaag gagattttga tacaaggttt agatgaagaa gtagcaaagc aatttaactt
    16321 agaaataata aggggtcgtt atcctattgg ggagaatgag actttaatag atgaggatgc
    16381 agttaaaagc tttaatctaa aaataggaga ttttatcaaa ataaaattag cagatggtag
    16441 aaataaaaat tataagatag taggtgaata ttcatcaata tcaagcttaa gagctacaga
    16501 taaacatgga ttaatacttt caatattggg tacacaagct attaaaaaat cagaaacgta
    16561 cgattattat tatattacat ttaaagaaaa ggttaatatt agaaaagcca cttctgatat
    16621 taaaaatacc ttcaatttaa gtgataaaca aattggtaaa aatgaaagat tactttctat
    16681 tataggtcaa ggtgactctg acttttctaa accattgtat ttaacagcag gattactttt
    16741 tgtacttgtt ttagtagcaa gtattatcat gatatataat agttttaaca tgagtgtaat
    16801 tgagagagtt aaattttttg gtctgttacg ttgtctaggg tcatcaaaac atcaagttaa
    16861 aaaatttgtt gttttagaaa gtttaatttt aagtataaaa gctattccaa taggcttagt
    16921 attaggaagt cttgtaacta ttatatcttc tatatttctt aagtatgtta atgagttatt
    16981 cagctcaatg ccaattttaa aagttagttt tattggtatt gcatttggaa taattgtagg
    17041 gtttttaact gtaatattat cagctattat tccagcaaaa aaagcttccc gtgtatctcc
    17101 acttagcgct gtacgtggga atctaatgtc caaagaaatt ttaaataacg ggaagacaag
    17161 taaagttgca aatataggaa gttttaaaat tgagacttct atgggattaa atcatgcttt
    17221 ttcaagaaag aaatcttttg tacttatgac ttcttcattt gccattagta ttatactatt
    17281 tttaggtttt agtgtgctta tagaccttat gtatcaagga cttaagccta tgaagccaga
    17341 agctcctcat atatctattg ttgaccctaa ttttaagtct gttttaacta aggaagatat
    17401 taatgaaata agcaatatca aaggagtaaa gaaagtatac agtagaatga tgacggatat
    17461 agaagctaca tataatggga agaagacaaa atcacaatta atttcttatg aaaagtatca
    17521 atttaattgg gcaaaaaaat atctattaaa aggagaagtt aatgaaaata aactacagtc
    17581 agaaaacctt gcattagtag aagaggggca tggatttgat ataggagata agatatatat
    17641 taaaaatgga aatgctgaaa aagagttaga ggtaggaggt atactttcct caattccatt
    17701 tgaggctaaa gataaatata ttggaaatat tataacatct gaaaatactt ttacgaatat
    17761 aacaggtata aagggttata taataatgga tattcaggtt gataaagata ctcctggaga
    17821 tttaattgaa aatataagac gtgtagtaga tgataactta gaggtcagag ataaaagaca
    17881 aagtaactta gaggttaaaa attcgtttta tactatggca acatttgtat atggatttgt
    17941 gtttattatt gcgattataa gcatgtttaa tatcataaat agcatgaata taagtgttac
    18001 aagtagaaag aattactatg gtgttatgcg tgcagttggc atatccaaca gacaattaag
    18061 aagaatggta atagtagagg caggttcata tgcagtgagt ggttgtttat taggtagtat
    18121 actaggatta atattacata ggtatatgtt tatatcgtta gtaagctcaa tgtttcattt
    18181 agagtggcaa gtacctttta atttactact tatcactgta ctaactatga taataataac
    18241 tttattatca gtaagaaaac caatcaaaaa gatttgtgaa ttagatataa tagactcaat
    18301 aaatgcacaa taaatatagg tattgattta aattaaaaaa acgaagtaaa aatgaaatag
    18361 aaaatatttt tcatttttac ttcgtttttt atattataaa atttttgtta tgttacaaaa
    18421 tttctataaa gatgatgtga atctaaacat tatatgacaa cgtgagcatt taagttaaaa
    18481 tattttaaat atactatttt agataagttc cctcttcatc ttgataaatt aatccttttt
    18541 tcataagcac tccaagagct cgtttaaaat ttttcttact tgaattaaat aatatagaaa
    18601 tatctttagg gtcagattta tcattaaatc tcatatatcc atcagaacct tctaaataac
    18661 ttaaaatctt attttctaaa gtatcaagtt catcctttct atttcctcta ggagaaagac
    18721 caagttttcc atcttcatat atttttatga catgtaaatt ttcaagaaca tcaccagttt
    18781 ttagttcagt aaagtactca ttatgaagta ttacaccagc atatttattg tctacacata
    18841 tcattgcaga attattagtt tggaatccat atacaactcc agttacaata tctcctacat
    18901 tgtaagtatg gtctgttaat aagtatgaat ctatatctgt agtagcagca attcttttgc
    18961 ttttgtctaa atatatataa aataaatatt tttcgtctct aaatagtgga taagtcttag
    19021 ctttaaaagg aactaatatg tctttaggaa gtcccatatc tataaaagta ccaatgtctg
    19081 tattatcaac tacttttaaa tgtgctacat ccccaacctt agctaaaggc ggtattaaag
    19141 ttgcagcagt tctgtcatca gaatctttaa atatgaaagc atttacttca tcacctatgt
    19201 ttatttcatt ttttccgatt aatctattat gaagaagtat atcgtctttt gtgttattag
    19261 tttgtccatc caaaaaatat ccaaattcag tttttctttt tactgtaagt ttattaaaat
    19321 cacctatgtt tatcaaatta aaatcatcct ctcattttat atttataatt tagccataat
    19381 atacttatgt agtagtttgt cacagtatct atattagtat tagtgaaatt tacaataaat
    19441 aatatagtta tttatgtatt tttcaattct ctagcttctt atatagtata aactccaatt
    19501 tcttttttgt ctttaggttt tattaaaatt tttacaaaat aagttaaaaa gtattgaaat
    19561 aaaatttctt ttaaagtata attggtatat accaaaattg gtatatacca tatcgaaaag
    19621 tggtgactat atgaaaatag taaataaatc tagtaatata ccattgcata ctcaattaag
    19681 ttcaataata agagagatga tagaaactgg tgagcttaaa gagggcgatg caataatgcc
    19741 agaaagagaa ctttgcaaca tacaaaatgt aagtcgtatg acagtaaata aaaccattgt
    19801 tggattagtt acagaaggac ttctttatag ggtacaaggt aaagggacat tcgttgcaaa
    19861 gcaaaaaaag aagtatcagt tttcaaatgt aaaaggtttt actgatgtta tgaaagaaaa
    19921 gggagtaaac ataaaaacgg atatattatc ctttgagatg gaactaccag atgatttagt
    19981 taaaagaaag cttggaataa gtgataatac tacaaatata tataagattg tcagacttag
    20041 atatactgat ggagagccat ttggattaga aatagtatat ctatcagaag aaatgtgtaa
    20101 gggtctaaca aaagggatat tggataattc ttcattatat agggttttaa atgaaaaata
    20161 tggctataag atacaaaaag ctgaacaggt aatggagcca gtaatacttt cagatgaaga
    20221 aagtaagctt cttgaaacag atgaaggtgc tttagctctt aaacttcata gaaattccta
    20281 taatagagaa ggtagaccta tagaatatac catatcaata tttagaacag ataaatatca
    20341 atatgaaata gtattgagtg agtaggtgaa taatatgaaa tgtttaatta atggaaagat
    20401 aatattaaaa aatcaaatct tagaaaataa agtattagta tttgatgaaa aaataattga
    20461 tatagctgat agtgtaccaa aagattgtga agttatagat gctgatggaa aatatatatc
    20521 tccagggctt attgatattc atatacatgg aaacatggga aaagatacaa tggattcgac
    20581 tgatgaatct attgaaacaa tttcaaaatc aattatgaga catggagtaa catcttttct
    20641 tccaactaca atgactatgg ataaagaaca tgtttatgat gcacttgaag ttattaaaaa
    20701 agcccaaaat agaaagcttg aaggggcaca agtattagga gcacatttag aaggaccttt
    20761 tataaatgag aattataagg gagcacaaaa tgaaaaattt ataataaatt caaaatacga
    20821 atttataaaa gaatataaag atgttataaa agttataact tatgctccag aaaaagatat
    20881 tgattttgat tttactagag aaataaaaag atgtacagat atagttttat ctattggtca
    20941 tagtaatgca aattatgacc aagctaaaga agctataaat ttaggagtaa ctaatgtaac
    21001 tcacatgttc aatgcaatga ctggtttaaa ccatagagac ccaggcgttg ttggtgcagc
    21061 acttacaact aatgtgtaca gtgagttaat agcagataca atccatataa ataaagattt
    21121 atttcaattt atactaaata ataaaggaaa agaaagactg attttaataa cagattctat
    21181 agaagctggt ggtcttgaag atggcaatta ttctcttgga ggtcagaaag tcatagttaa
    21241 gggtaatgaa gctagacttg aaaatggagc attagctgga agtgtgttgt ctttaaataa
    21301 aatggtattt aacttcttaa ataatacaaa ccttaaagtg aatgaagcaa ttaatttagc
    21361 ttctttaaat ccagcaacat ctttgggaat aaatgacaaa aaaggaagct tagaaatagg
    21421 taaggatgca gatatagctg tatttgatga gaatttagat tgtaaaatga cactttgctt
    21481 gggggaagta gtatataaaa atatatagta tacaaaacaa accttaaaga cagtaaaaac
    21541 aatataaaaa ataataaatt aaacttacat tagtaaaagt agtagttaaa atttaggagg
    21601 aataagatga gagtattagt gtgtaaagat tatgatggaa tgagtaaaaa agccgctgaa
    21661 atgatagcag ctcaaatagt tttaaaacca aattcaatac tgggactagc tacaggaagt
    21721 acaccagtag gtatgtatag agatttagta aaaaaataca atgataatat agtagatttt
    21781 tcagatgtga tgagctttaa tttagatgaa tattataagt taccaatatc taatgaccaa
    21841 agctatgatt attttatgaa agagaatcta tttaatcatg taaacataaa gccagaaaat
    21901 actcatctac caaatggaat ggctgatgat attgaaaaag agtgtatgaa ctatgaagct
    21961 tctatagatg cagctggtgg tatagatgta caagtacttg gaataggtcg taatgctcat
    22021 ataggtttta atgaacctga tactaaattt gcaaaaagaa ctcatgtagt tgaacttaca
    22081 gaatctacaa tagaagctaa tgcaagattc tttaaatcaa gagaagatgt tcctaaaaaa
    22141 gctgtaagta tgggaatagg ttcaatattg aagtctaaga aaatattgct tttagcttca
    22201 ggagaagaaa aagctgatgc agtatataat actgtttacg gagatataac tccagaagtt
    22261 cctggttcta tacttcaact tcataaagac actatagtta tagtagatga agcagcagca
    22321 agtaaattga atccaaaaga ttataaatta gtataagaat agaatgtagt ttaaataaaa
    22381 aatagctatc attgaataat caaatttatt taatgatagc tatttttatt taaattagtt
    22441 attattgttt aaattacttg cttattactt tttacgaaaa ctcgttatca agaagtatcc
    22501 aaataagatg ttaaaaatta ttatgtttag tgggattcta tcaagcataa taaaattacc
    22561 tataattggg atattaccaa ctggaaacat aaagaagaat acaggcatca gtgatagtaa
    22621 aaaagatgga aaacaaagaa gaagtaatct tggaatatca atataccaac taccatcctt
    22681 tttgtattct ctaatcaata attcagttcc tagtaataaa ccaataccac cagaggaaat
    22741 taaattaata aaaatgtatc ttttaaagtc ttgcatataa ccagtgtaaa attgagtata
    22801 gaaatcataa tttaatttaa cggctaaaaa atacataagc aatagaatga ttaaaaataa
    22861 aagatatcta agagcttttt tcattttaaa ttctcccttc agaaatactt tatttagttt
    22921 tattttgttg gaatctgatt ttatcattta gtttaagaaa tccataatat tagtgaagaa
    22981 tgaaataagt attatatatg aataaatatt tttagaatta aggtgaattg tagtgcttaa
    23041 tataactata atattaattg tttgaatatt caataaaaag ttcgaaaaat atcaattctg
    23101 atataaactg aataaataca gttgaatatt aaattattat tttatcaaaa atatgcaaag
    23161 tactacacct tcaaatatca cctaaagtaa ttaaaaggca attaaagtga agtagttcaa
    23221 gtatttctaa aataagttta ataaattcta taataattta ttttttgcct taaaattgcc
    23281 ttaaaatcca aacaatttta aaactaatgt actataaaaa gctggtataa tttaaatata
    23341 gtaaaaaata agatttatct tatctattgg gatttaatta tttaatcaat atatttatac
    23401 catttaattt aaataaaaag tctattaagg agtgagctta tgagtaagtt aatttatata
    23461 gcagatgatg aagataatat aagaaatttg gtaaagacat tcttaaaaaa tgaaggtcat
    23521 gatgttatgg attttaaaac gggtgatgaa cttttagaac aatttaatat aaaggagtgt
    23581 gacttagtaa tactagatat aatgatgcct ggctcaagtg gttttgaagt atgtacaaaa
    23641 cttcgtgaga aaagtacagt cccaataatc atgcttactg caagagatac agatatagat
    23701 tatattacag ggataacctt aggtagtgat gattatttta caaaaccatt tagcccaatg
    23761 tctttggtta tgagagtaaa atctatattt agaaggatag agtttgaaaa gaaacaaaat
    23821 tatgataaat atagtaactc gatagatatg gaattaaaat ttggtgatgt aataataaat
    23881 aaaaagaata aaatcgttac aagtaaaaat gtaaatatag accttacacc aaatgaatat
    23941 aacctattga cttatttgtt tgaaaatata gatagagcaa tttccagaga tgaattatta
    24001 aacaaaatat ggggatacga tatagaagtt gagacaaggg ctgctgatga tacagtgaag
    24061 agattacgta aaaaaatttt agatacaaat attttaatag aaactgtatg ggggtttgga
    24121 tttaggttga aggagaaaag ttaagatgaa gaatggaaat gacaagaata gaattggaaa
    24181 gattaaatcc aaatttaaat taaagttctc aaagaaaaat ataagcataa aaactaggat
    24241 tttgttgagt atactttgtt tgataattat agtatttatg ataatattag tatcttttaa
    24301 tgtatttctt gatacatata taaaaacaac tgcgaatgaa gaattgacaa agtctacaga
    24361 aatagtagaa catatggata gtaattttag acctattaag ccaccacaag atgatggaaa
    24421 gcttcctccc aaagtattat ctaattttat gagaaatgta caagataaag ttaggatggc
    24481 agaaacacaa tcagatgctg atgctatggt agttgattct aagtataacc tgatatttcc
    24541 aacaagagag gacgatttct taaaaaatgt tgatgagatg gagctgatta gggagtgtat
    24601 tcaaaatgaa aaattagatt taaattcaga tgcaaataca cggatatcta ccagaaataa
    24661 ggattattat atttcaactg taaaaattac cagtatatat ggtgaacaag acaagtatct
    24721 tatacttttt atagacatat caaaaactct aaatctagca caaaagataa atattgtatt
    24781 aatatctgta atgtgttttg ctggaatatt agcaatattt acagctgtga ttttatctga
    24841 gaaaattgct aaaccgataa aagagttgtg tgagtttgca aaaaaaatgg gtcaaggaga
    24901 tttcaaaaga acctactttg acttttcaga taaagaatta gtagagctct caactgttat
    24961 gaataagagt gctgagtatc tggataagta tgataatgaa caaaaaatat tttttcaaaa
    25021 tgcatctcat gaattaagga caccacttat gtctattaaa ggttatgcag aagctataaa
    25081 atataatgtt atagatagta aacatgcaag tgatataatt cttgaagaaa gtgataggtt
    25141 aagtgatatg gttgaagatt tattatacat ttctaaaata gataatataa ctaaagattt
    25201 tgaacttgta gaatgtgatt taagagaagt actttcaaac tgtggagcta ggcaaaatgt
    25261 aagagctata aataagggga taaagtttat ttatgaattt gatgaagaaa tggttttatt
    25321 tgaatgtgat gaaaaaaaca tttccaaagc atttatgaat cttatagaaa atgctttgag
    25381 atacgctaag agtgaaataa aaatagtgtg taagtataat caaaaaaata tagttgtgat
    25441 aatagaagat gatggaattg gaatcaagaa agaagattta cctcatgttt ttgaaagatt
    25501 ctataaggga gtaggaggaa atcatggaat tggattatca atagttaaat ctattgtaaa
    25561 taagcatggt gggcgtatat acgtagaaaa tggcaaaaaa ggagctaaat ttactatatt
    25621 atttaaattg taaaaaaatt agagaaaata tagagatgtt tgaagttgtt ttttatatat
    25681 atctgttgta tactgttgaa aaaagaacaa tattaagctg acatatatta cgatagttga
    25741 catttaaagt aaatgcttaa atatagatac gaaaggaaaa gtaaaatctg aagtgtattt
    25801 aggtttaagt aagcttctta ttttataaaa aatatgtatg aaagaacagc tgatttttaa
    25861 tattacaaat ttagtaacgg ttgtatttga agcttttgtc atccatatgt ttttatcaga
    25921 ctttttaggc cagaaagaag ggtatataaa agttgtaaga tatgctaaac tgggttttat
    25981 aatatgttta ggattctgta atttaataac tttaaaccct aaaatcacaa tgcctttaat
    26041 tttcatcctt attttttcaa catcattttt atataaagga aatttaaaga cacgattgtt
    26101 tactactgtt ttattaagta tattctttat actgtcagaa attgtagtta caagtatttt
    26161 tgttttattt gtaaaagaag gctttgaaat aatgcttgaa aataatagta taagagtatt
    26221 ggcgacaatt ttatctaaaa ttgtcttttt gttgacttgt aaaattattt gtctatttaa
    26281 aaaagatgta catttagata tgcccataaa gtattggctc cctttatttt taatacctat
    26341 atttagttta tttctgtctg tttctatctt tgatgtaagt aaatttttta gtcttgaaag
    26401 tttaaaattt ctaagcttaa tatctagtgt tggtatttta tatattaatt ttattgtctt
    26461 ttatttattt aaatttatta tagataaaac taaactatct atgaaatatg aactacttga
    26521 aaaagaaata attcataagg aagaattaag acttagtaat gagtgttata aaattattgt
    26581 tgaacagact gattctgtcg ttttcgaatg gaatatcaaa gaaaataaat catttgtatc
    26641 gcaggcttgg actgaaaaat ttggatataa caatgcatgt aagaatattt ttaaagaaat
    26701 taaagataaa gacttagttc attctgaaga taaagctata tttgaaggtt ttcttgaaag
    26761 tattaaaaaa aagaatatgc acaatcaagc agtatatcgt ttaaaaaaat ctaatggtga
    26821 atatatttgg tgtagaacat caattacaag catttataat gatgaaaatg aacttttaag
    26881 agttgtcgga gtaattgtag atgtagatag tgatataaaa aaatatgaag agttaagaac
    26941 tagagcagaa agtgactcat tgaccaatat atacaataaa ggtacatttg aaaaattagt
    27001 ggaagaaact atagttatga atacaggtga taaaaaagat gcattattca ttattgattt
    27061 ggatgatttt aaagaaataa atgataattt tggtcatcca tttggtgatt ttgtactaaa
    27121 aacatttgct gataagatac aaactagttt tggtagtaaa gacttagtcg gaagaattgg
    27181 tggagatgaa tttgttgtat atatgcaaga ttatgttaca gaagtaaatt tgcataaaaa
    27241 agctaaagaa ttaaatagag tactttcgga taattacact gatttgagtt ttagttttga
    27301 tgcttctgtc agtataggta ttgcaaggta tcctcaagat ggaacttcat tttttgaatt
    27361 gtttaaaaat gcagatagag cactttatag cataaaagca tcaggtaaaa attcatattg
    27421 tttatttgaa gaagaactat atgtacagtg aatttgaatt tagattttat gtgagagtaa
    27481 gatttatata tacttgtggc tgtctgattt ataaaatatg ttactttgta tatttttata
    27541 tgtttcagta aggttaatta acataagcaa aggtgtaagt aaatttatat tagtttaagt
    27601 ttacttacac ctttataaaa atacaaataa tagattcatt tacattctta aatgattaaa
    27661 gctctttacc ttctaaacat gcataacata tcgttataat tccagtaaat aagactaaaa
    27721 tacccattcc taagattaca gtttcattat ctattactcc aaatgttttt aaagtaactt
    27781 atttcgcttt gtatcataat aatttgtctc tttttacatt ttaacttctg ctgagatttg
    27841 agttagatat tcgttagaat ctcgcacact tatatcatat agaagatatt cttcataaac
    27901 aaaatcccca atacgtaaat tattctgatt aaaaaaattt aataactttt cataagcctt
    27961 gtaaatagtt tcataactcc ctttatgata agcagttaca taaagtccct ttggtttgat
    28021 tgatacagga tatttcttgt actctttatt aacttttgta aataaatatg aatatgattc
    28081 aaagtcttta ttaattatat cttcaccttt aatgattgag ccaatcgtac cataatcact
    28141 caattcatag ttgtccataa agttaataca ttcatataaa aaattttctt cctcatctcc
    28201 taaaaactgt tctttatatg taacaggtgt tttgaccaag tactcttctt catgttcttt
    28261 taaagtaata gtattcgcat ctatattaca ggcattttta gtgaatgaaa tagtagattc
    28321 cattagagct tgaatattat ttagtttttc aatttcattt ttaatatcaa tggttttctt
    28381 ttctaaaagc tcaattagta aattaggtgt tcttttgtct atataagctt taatttcttt
    28441 taaaggcatt tttacttccc taagtgaaga aattatacca aaaatgctaa gttgatggta
    28501 tgagtagtat ctatatccat tttctttctt taattctggt gagaaaagac ctatatcatc
    28561 ataatggaaa agtgtttttt tattgattcc acaaatcttt gcaaattctc cagttgtaaa
    28621 ataatttttt gaaacttgac tcataattat aattcctcct tgactatcct gttacagtat
    28681 agtttatcat agtattttgt aaaggagtgt gattatatga aaaaattaat tcatttttta
    28741 aaaccatata gagttttaat tgttgtagtt ttgatattta cgtttttaca aacacttgga
    28801 actttgtata taccaacact tacagcaaat atagtaaaca atggtgtagt aaagggagat
    28861 atagattata ttgtaaaaac aggtttaatg atgatgatag tagctggtat aacagcttta
    28921 tcagctgtgt tagtttgtaa agtatcagca aatctttcat ctggattttg tagagatatt
    28981 agagaagcag tatttataaa atcacaagat ttatcaataa atgattttaa taatataggt
    29041 acagcatcaa tgattactag gagtactagt gatattactt taataggtca gtctgtgttt
    29101 atgtttattc aattagtatt accagcacca attataactg tttctggtct attcttagca
    29161 tattctatag ataaagcaat gacaattatt attgttgtag taatgttttt attcatgctt
    29221 tcagcatttt tagttggtaa aaagcttata aagttgttta aaatgatgca aataaaaatg
    29281 gacaatatga atagggtgtt acgtgaggta gtaactggag taagagttat aagagctttt
    29341 aataggagtc attttgaaaa aaagagattt gatagaactg caatagatta ttctgaaaca
    29401 gcaatttcaa taaataaaat atttgcagta cttatgccta ttgtaatgtt aataatgaat
    29461 ttaggtatag taagtattat ttggtttggt gggatgagag tcagtaatgg aaatatggaa
    29521 ataggtcata taatggcact tgttgaatat tgtatcttaa ttttattcta tttaatcatg
    29581 ggagtaatgg tatttatgta catacctaga gcaggtgcat gtgcagatag agtaaatcag
    29641 atattagata tagaacctga aattgtagat ggaaatggtc ataaagatac tgtctcagag
    29701 agaggacatt tagtatttaa aaatgtaacc ttttcatatg cacaatcaga agaacctgtg
    29761 ttaaataata taacatttga agcaaaatct ggagaagtaa ctgcaattat aggaagcact
    29821 ggctcaggca aatctactat tgcaaatata ataccacgtt tttttgaaat tcaatctgga
    29881 gaaatatcta taaatggtca ggatattaag aagattcctc aaaaagaact tagagataag
    29941 attggatttg taccacaaaa agctttttta tttagtggaa caatagaaga aaatataagg
    30001 tatggaaaag aagatgcaag tattgaagaa gtaaagcatg ctgcaagtat agctcaagct
    30061 gatgaattta tttctgatat ggaagacaag tatgactcat ttgtagcaca aggtggaaat
    30121 aatttgtcag gtggtcaaaa acagagaata tccattgcaa gagcgttagt tagaaagcct
    30181 gaagtatatg tctttgatga tagtttttct gcattagatt ttaaaacaga taaaagactt
    30241 agaaaagcgt tgaaaaatga aatcaaagat tcatcagcta taattatagc acaacgtata
    30301 agtacaatca tggatgctaa tcagattata gttttaaatg atggaaaaat tgtaggtatt
    30361 ggaaaacata aagatttact tgaaaattgt gaagtttata agcaaattgc ggattcacaa
    30421 cttagtaaag aagaacttgc ataaaatgaa gaattaatct gatatttgga ggtgtatgat
    30481 atgagtgaaa aaagaataga tgaacaagat atagaagtac ttagttttga agatggcatg
    30541 tcagcagaaa aagctaagga tgcaaaaaaa actactaaga gattattaaa atatatggca
    30601 aaacaaaagt ttaaaatatt aattattttt atttcagtat tgataagttc agcgcttaca
    30661 gttttagcac ctatggttat gggaaaagct attgaccaac tatttaatgg aataaaaact
    30721 gcagtccaaa caggtacaaa attcagtgtg aattttagta ctatgggggg gatagtatca
    30781 atacttctag ggttgtactt aattagtgca gtatttatat atattcaaca gtatattatg
    30841 tcaggagttg cacaaaatct agttcttagc atgagaaaag atttaagtga taaactcaat
    30901 aaacttcctc ttaagtacta tgattctcat aaaaaggggg aaacacttag tatagtaaca
    30961 aatgatttag aaaaagtagc agatagttta caagaaggat taatgcaact tattacagca
    31021 gtagttacag tagttggttc tattgtaatg atgataagta taagtgtacc acttacaata
    31081 gtatcagcta taacattact tgtttcattg ggtattactg ttgtaattgc aagaaaatca
    31141 caagaaagat tctctgaaaa tcaaaaggca ctaggagagt taaatagcaa tatagaagag
    31201 atatttactg gtcaaatagt ggttaaagca tttagtaaag aaaaggatac tataagaaac
    31261 tttaaaatat taaatcaaaa tctttataat gcaagtagaa aagcacagtt ttcgtcatat
    31321 gcaatatctc caattattag atttataaac ctaattggct atgttattat agcagttgta
    31381 ggtgggatat ttgcatcaac tggagcaatg actcttggta gtatacaagc gtttattcaa
    31441 tatgtaaatc aagcatcaga gcctactaca gaaatatctt atattgtaaa tatgcttcaa
    31501 gcagcaatag catctgcaga gagagttttt acagttatgg acgaggtgga agagataaag
    31561 gatgaagaat cttcaaaggt tattagcatg cctaaaggta aagtacaatt tgagcatgtt
    31621 aaatttggat atagtgatga ttttattctt atgaaagata ttaatatcaa tctaaatgca
    31681 ggagataaga ttgcaattgt tggacctaca ggagctggaa agacaactct tgtaaattta
    31741 cttatgagat tttatgagat acaaggtgga agaattacta ttgatggtgt taatataaaa
    31801 gatttaaagc gtggagaact tagaactatg tttggtatgg ttcttcaaga tacatggctg
    31861 tttaatggaa gtataaaaga aaatattgca tatagtaagt cagatgccac aatggatgaa
    31921 attgtaagag cagcaaaatc agcaagagta gaccatttta taaggacttt accacaaggg
    31981 tatgacacta tattgactga ggatgcttct aatctatctc aaggtcaaaa gcaacttcta
    32041 acaattgcca gggcaatact ttcagaccca tcagtattga ttcttgatga agcaacatct
    32101 agtgtagata ctagaacaga ggtagaaatt caaaaggcaa tgaataactt gatgaaggga
    32161 agaactagtt ttgttattgc acatagatta tcaactattc gagatgctga tttaatatta
    32221 gtaatgaaag aaggaactat tattgaacaa ggaagccata aggaacttat tgaaaagaaa
    32281 ggattctatg aagaacttta taacagtcaa tttacaagtg aatatgatga agatgttgta
    32341 tgattatcaa gttttaggca actaaatgat tgataaatat taagttaaat atagtaacat
    32401 gtaaggttat atggaataag agtatttctc tatttcatat aacctttttt atttatcaaa
    32461 ataagataaa atttcctttc taaattagat tgaagaatgt atttatcaat ataaaagtaa
    32521 aataatcatt attattagtg aagtaaatat tgtttgccac atatatgcca taaagatgca
    32581 cttattttac ggagatttaa caaagtagtg taaagtatac tctaaatata aaataaatca
    32641 aatagagtta aataaattga gaggtgatat aaatagtgaa taaaaaattg aactatagat
    32701 ttgaaatgaa gcacaaaata actgaagcag atgtgttagc tttaaaatca agactctatc
    32761 aaataatgaa gaaagatgaa aatgcatcta aagatggaaa atatcttatt aggagtttat
    32821 attttgatac tcctgaagat aaagcacttt tggataaatt aaatggcgtt gcaataagag
    32881 aaaagtttag gataagattt tataataatg actgctctta tataaggcta gagaaaaaaa
    32941 tcaaacatta caacatgaca tcaaagctat caactaattt aaccaaaaaa gaagtaataa
    33001 atatactaaa taatgatatt gaatttctaa aagagtcgac aaattgttta ttaagagaat
    33061 tttatttgaa attaaaatgt gaaagattag aggcaaaaag catagttgat tacaatagag
    33121 aagcttttat ttatccagtt ggaaatgtaa gagttacaat cgatagtgat attaaaactt
    33181 ctgtaaattc ggtagattta tttaataaag atttgccaac tgtttcggta attgatgaga
    33241 atatgactgt attggaggtt aaatatgatg aatttatacc agattttata aaggatttaa
    33301 ttcaaattaa taagactacc tctacagcag tttcaaagta tgcttcaagt agattatata
    33361 tttaagaata aaggctatga tttatataaa gatatacata aataaggtta aaaaacaaat
    33421 aagttaaaaa taaataaaat aaaaaataaa taagttaaaa ataggaagga tgattttaat
    33481 tatgacattt aatgatattt ttaaatcaag ttttatagaa aacgtatcag gtttttcatt
    33541 tgtggacagt gctttagcac taggttcagc ttttttagta ggtttgttta tatatatggt
    33601 ctataaaaag acttatatgg gaattatgta ttctcgacca ttcaatgtgt cgcttgtagc
    33661 acttacaatg cttacaactt ttgtaatatt ggcagttact tcaaatgtag ttttatcctt
    33721 aggtatggtt ggtgctttat ctatagtaag atttagaaca gcaattaaag acccaatgga
    33781 tttagtattt ctattttggt cattgggttc aggtatagtt ttaggagcag gtttgattcc
    33841 cttagctgtc atgggttcaa taattatggg attgattcta atattctttt caaataaaac
    33901 aatatctgaa actccatata tattgatggt aaattgtaat aatgaagatt cagaagatat
    33961 agcaactgat aaaattaaaa aagtatttac taaatatcaa ataaaatcaa aaagtgttac
    34021 accagaaaaa ggaatagaat tagtgtttga agtaagaatg aaagatggag aaacaagtct
    34081 tataaatgac ttgagtcaag tagatggagt tactaatgca gttcttgtaa gttataatgg
    34141 tgactatgta gcatagagag gagtaatctt ttatgaaaga taaaaaattt acccttctta
    34201 tctcgattat gattatattt ttatgtgctg tagttggagt ttatagtaca tctagcaaca
    34261 aaagtgttga tttatatagt gatgtatata ttgaaaaata ttttaacaga gacaaggtta
    34321 tggaagttaa tatagagata gatgaaagtg acttgaagga tatgaatgaa aatgctataa
    34381 aagaagaatt taaggttgca aaagtaactg tagatggaga tacatatgga aacgtaggta
    34441 taagaactaa aggaaattca agtcttatat ctgtagcaaa tagtgatagt gatagataca
    34501 gctataagat taattttgat aagtataata ctagtcaaag tatggaaggg cttactcaat
    34561 taaatcttaa taactgttac tctgacccat cttatatgag agagttttta acatatagta
    34621 tttgcgagga aatgggatta gcgactccag aatttgcata tgctaaagtc tctataaatg
    34681 gcgaatatca tggtttgtat ttggcagtag aaggattaaa agagtcttat cttgaaaata
    34741 attttggtaa tgtaactgga gacttatata agtcagatga aggaagctcg ttgcaatata
    34801 aaggagatga cccagaaagt tactcaaact taatcgttga aagtgataaa aagacagctg
    34861 attggtctaa aatcacaaaa ctattaaaat ctttggatac aggtgaagat attgaaaaat
    34921 atcttgatgt agattctgtc cttaaaaata tagcaataaa tacagcttta ttaaaccttg
    34981 atagctatca agggagtttt gcccataact attatttata tgagcaagat ggagtatttt
    35041 ctatgttacc atgggatttt aatatgtcat ttggtggatt tagtggtttt ggtggaggta
    35101 gtcaatctat agcaattgat gaacctacga caggtaattt agaagacaga cctctcatat
    35161 cctcgttatt aaaaaatgag acatacaaaa caaaatacca taaatatctg gaagagatag
    35221 taacaaaata cctagattca gactatttag agaatatgac aacaaaattg catgacatga
    35281 tagcatcata tgtaaaagaa gacccaacag cattttatac ttatgaagaa tttgaaaaaa
    35341 atataacatc ttcaattgaa gattctagtg ataataaggg atttggtaat aaagggtttg
    35401 acaacaataa ctctaataac agtgattcta ataataattc taatagtgaa aataagcgct
    35461 ctggaaatca aagtgatgaa aaagaagtta atgctgaatt aacatcaagc gtagtcaaag
    35521 ctaatacaga taatgaaact aaaaataaaa ctacaaatga tagtgaaagt aagaataata
    35581 cagataaaga taaaagtgga aatgataata atcaaaagct agaaggtcct atgggtaaag
    35641 gaggtaagtc aataccaggg gttttggaag ttgcagaaga tatgagtaaa actataaaat
    35701 ctcaattaag tggagaaact tcttcgacaa agcaaaactc tggtgatgaa agttcaagtg
    35761 gaattaaagg tagtgaaaag tttgatgagg atatgagtgg tatgccagaa ccacctgagg
    35821 gaatggatgg taaaatgcca ccaggaatgg gtaatatgga taagggagat atgaatggta
    35881 aaaatggcaa tatgaatatg gatagaaatc aagataatcc aatagaagtt ggaggttttg
    35941 gtaatagagg aggaggctct gtgagtaaaa caacaacata cttcaaatta attttaggtg
    36001 gagcttcaat gataataatg tcgattatgt tagtaggtgt atcaagggta aagagaagaa
    36061 gatttataaa gtcaaaataa gattaaaatt gaatataaat aattttaata ctgttaagtc
    36121 taaatattta cttagcagta ttttttagaa aataatgtgt ttgattaata atataaggta
    36181 ataaattgat aaaaattaaa tacagcatat taataataca taataaagta ataaaaaact
    36241 gtttaagtat aataacttat atttagacag tttttttcat ttataaataa tataaatgaa
    36301 attgaaaaca tttgaaggat agaaattggt tggataaatg tataaattac ttcaccaaaa
    36361 aataatgaca aaaactgtaa ctaagttgta ataaaatggt agttatttag cataaaataa
    36421 tgtatcatag aagtatatta taaaattaat aaaataagat ttttgtaaga aaactgctgg
    36481 cttttgatat ttagagtgag tagttaaatt atgtttagga ggagtagaat ttgttgcaaa
    36541 aagtattatg gaaattttta aagctagtag tcggtttatt tatatgttca gttggtatag
    36601 tattgacgat aaattgtaac cttggattat ctccctggga tgtatttcat caaggacttt
    36661 caaatcatat aggaataact ataggaactg cttctataat tgtaggctct atagtagtta
    36721 ttgctgatgt cgttcttgga gaaaatgtag gatggggaac tgtttttaac atgctactaa
    36781 ttggattttt tatggattta cttttatata gtaatttaat tccagaggct gataatttat
    36841 ttgtaggaat aattatgttg atattaggat tggttctttt gtctgttgga atggtatttt
    36901 atatgggaag tggtttagga agtggaccta gagatggttt gatggttgca attcaaaaga
    36961 aaacaggaaa atcacttaaa ttaataagag gtactataga agttggagca ttaatagtag
    37021 gatttttatt aggtggaaaa gttggtatag gaactataat aagtgcattt ggattggggt
    37081 atttcacaca aatggtattt ggcttattta aattagattg tagtaagatt aaacatagat
    37141 ttatagtaga tgatataaaa tttatcaaag tgtatatgag tggtgagaaa aaagctgctg
    37201 ttcaaaatat agttaagtaa tttaagaata aattcaacga aatgttaagt attttaaatt
    37261 aacattttgt ttagatagat aaattgaata ttaaaagaat attaaaatat gaggattgct
    37321 agaacactat aaaatataga tttctagcaa ttttttattt gttcaatatt tttttagtac
    37381 tagaagtctt attctctatt taatgaatga attaatttat aattagagat actaaacttt
    37441 atagatatta atataaacac ctttaaactg tgtattttcc aaagccttta aaatgcttgt
    37501 atttattcta aataaaaaaa taaaaaaatg tcaaaaaaac tcttgtatta tatccaaatt
    37561 ttatatataa ttacctatgt tgtttagaaa ttttaaactc aatgtttagt aaacaaaatg
    37621 tttagtaaaa taaaacagaa actaggaggt gcaacatgga tataggtgaa aagataaaaa
    37681 gattaagaac agaaaaacaa ctgacccagg aagagttagc taatagatgt gaattgtcaa
    37741 agggatttat ttctcaatta gaaaataact taacatcacc atccatagct acccttatag
    37801 atatacttga aatactaggt acaaatttaa gggaattttt taatgaaata gatgatgaaa
    37861 gaatcagttt tacaaaagaa gatatgtttg aaactgaaga tgaagattta aaatataaat
    37921 taaagtggtt aattccaaat tcacaaaaaa atgaaatgga gccaataata ataacattgt
    37981 atccaggagg acagtataaa gaagaaaagc cacatgaagg tgaagaattt ggatatgtat
    38041 tagcaggttc aatatatgtc catataggcg aaaagaaaaa taaagtaaaa aaaggagaga
    38101 gtttttactt tagaccgaaa gcaaatcact atatatcaaa cgagggcaaa actacagcaa
    38161 aagtgatttg ggtaagtacg ccaccatcat tttagaatag aggtgtttaa taattggtag
    38221 aaaatataat agaattaagg gatatatcta aacattatga agaacttact gttttagata
    38281 atttcaattt agatataaag aaaaatgagt ttctaacatt gcttggacca agtggatgtg
    38341 gaaaaactac aacattaaaa ataatagcag gatttgagta tgctgatgat ggtaaagttt
    38401 tatttgaagg taaggagata aataatttgc caccatatga aagacaagta aacacagtat
    38461 ttcaaaaata tgctttattt ccacacatgg atgtttatga aaatgtagct tttggattaa
    38521 aaattaaaaa gactccaaaa gatgtaatag atgctaaagt aaaagaggtt ttgaaacttg
    38581 ttgcactaga aggttttgaa agacgacaag ttgaatcatt gagtggagga caacaacaaa
    38641 gaattgcaat agcaagagct ctagtcaatg aaccaaaggt attattacta gatgaaccac
    38701 ttggagcact tgatttaaag ttaagacaag agatgcaaat agaattaaag agaatccaaa
    38761 aaaaattagg aataacattt gtttttgtaa ctcatgacca agaagaggct cttactatgt
    38821 cagatactat tgttgtaatg tataagggca aaattcaaca aatgggaaca ccacaagata
    38881 tatacaatga acctaaaaat tcatttgtag ctaagtttat aggtgaaagt aacatatttg
    38941 atggaattat gatagaagat tataaagtta atttctgtaa tagagatttt gagtgtgttg
    39001 ataaaggatt cgaaaaaaac gaaaatatag aagtagtaat aagaccagaa gatataaaaa
    39061 tggtaaagcc agaagaagga atgttaaagg ggaaagttac atctacagtg tttaaaggag
    39121 tgcattacga gatagaattg aatgaaaatg gaagattgtg gatacttcat aatactaaaa
    39181 atgctgaggt tgggactgaa ctaggcatgg atatatatcc agaagatatc catataatga
    39241 gaaaagagaa taactaagat gaaaagaaaa tcttttttag catatccata tgtagtttgg
    39301 agtgcgatat ttgtaataat tcctttaata ttagtagtat tttttagttt tacaaaagaa
    39361 tcaggtggag gttatgcctt tacacttgag aattataaag aagtaattga ccctatatat
    39421 atgaaagttt ttgggcgttc tattctttta gcaggaggag ctacattaat atgccttata
    39481 gttggttatc cagtggcata tataatttca aaggctcgtg taagtagaag aggttcatta
    39541 atattattgt ttattcttcc tatgtggatg aactttcttt taagaacata tgcatgggtt
    39601 gctatattag gaaaaaatgg acttttaaat acatttttag gttggtttgg aatacaacca
    39661 ttagcaattt tgtatactaa ttttgctata cttttaggta tggtgtataa ctttttacca
    39721 tttatggttc ttccaatata tacagcacta tcaaagatgg ataatgattt gataaacgct
    39781 gctcatgact tgggtgcaaa taatatgaca gtttttagaa aaattatatt tcctttgagt
    39841 ttgccaggtg taatgtctgg aataactatg gtatttatgc cagcagtcac tacttttgca
    39901 atatcaagac tgttaggtgg aggtaaaata atgctggttg gagatttgat tgaacaacag
    39961 tttacagttg ttggagattg gaactttggt tcagctatat cgatatttat gatgatagta
    40021 atattaatat ctatgtctat aatgtcaaaa tttggagatg aatctgacaa agaaggcggt
    40081 gggttactat tttaaaatta aagaaaataa cttctaatat atatttagca ttagtattct
    40141 tatttttgta tgcacccata tttgctttgg tactattttc tttcaatgat tcaaaatcaa
    40201 tggcaagatg gaatggattt acatggaagt ggtatggtca attacttcaa aatgagagta
    40261 taatgagtgc tttatattat actattgtaa tagctatatt agcgtctgta atatctacaa
    40321 tagtaggaac tataagtgct ataggaatac ataagatgag aggtaagagt aagaaattaa
    40381 tactaaatgt aaactatctt cctattttaa atacagagat agttacagca gtagctctta
    40441 tgagtctgtt tgtatttgta aaaatggaat ttggatttac aacaatgcta ttggcacata
    40501 taatgttttg tttgccatac gttatacttt cagttttgcc aaaaatgaag caattacctg
    40561 ataatataga agatgcagct atggacttgg gagcaacacc tatatacgct cttagaaaag
    40621 taattttacc acaaataaaa ccaggtatag tttctggatt tttaattgca tttactatgt
    40681 caatagatga ctttataata agtttcttta atgctggaaa tggtgtaagc aatctttcta
    40741 tagaaatcta tggaatggct agaagaggta taaaacctga aataaatgca ttatcaacaa
    40801 taatgtttgc agtggttcta ggtttacttt tactagctaa taaaaaagaa tctatagtaa
    40861 gggggattaa ataatgatta agtttaaaaa agcaatatcc cttatagctg taggtatgat
    40921 gacagttact ttatttacag gatgtaataa agcaactgat tctacaaaag ttttaaatgt
    40981 atataatgtt ggagattata tagatgagtc tcttatagat aagtttgaaa aagaaacagg
    41041 aatagatgtt caatattcaa cttatgatac taatgaaatg atgtatcaaa aagtaaagag
    41101 tggtagtaca cactatgatt tagtatttcc ttcagactat atggtagaga aaatgaaaaa
    41161 tgaaggtctt ttggaaaaat tagattttaa gaatatacct aatatgaaat atttagataa
    41221 gagtttttta aatcctgtat acgacaaaac taatgaatat agtgttgcat atatgtgggg
    41281 aactcttgga atattgtaca ataagaaaga agttaaagac cctatggata gctggaatat
    41341 actttggaat ccaaagtata agggaaatat aatgatgttt gactctgtta gagatactat
    41401 agggataact cttaaaaaat taggatatag catgaatagt gtaaatccaa aagaaataaa
    41461 tgaagctaaa aatcttttaa tgaaacaaaa ggatttagta ttggcttatg taaatgatga
    41521 aggtaaggat agattgcttg gtggagaagt ggcaatgggt atgctgtatt ctggagatgc
    41581 agttacacta atggagcaaa atcctgacct tgattatgct atacctaaag agggaactaa
    41641 taaatgggta gatgctatgt gtgtacctac aacagcacaa aataagaaag aagctgagct
    41701 gtttataaac tttttattag acccagagaa tgcaaaagta aatgctgagt acattgggta
    41761 ttcaacacct aatacaggag cactaaaatt attagaccca gaaataactc agaatcctgt
    41821 tgcatatcca tctaaagaag tacttgataa atgtgaaact tttgaagata ttggtgaaaa
    41881 tataaaatta tatgacaagg cttggataga gttaaaatca aaatagggtc ttgttgtttt
    41941 aagtaaattt acttaataaa tacttaataa aactgttctt ttttagaaaa tttattttct
    42001 aattattgaa cagttttatt ttttgtttaa gttatattca tatgcaattt aaatagttta
    42061 ttaataaatg aaaacttatt tgtataatga ctttaataat aattagaaaa aatgggcaac
    42121 ttaatagaat aattggaggt gtaaatttat aaaaaatgaa taggataaat aaaaaaatag
    42181 atttattaat attggctttg ctcgttttta tatttttaat tgttggaata gtaatactat
    42241 cgattaggac agaaaataaa ttgaatgtgt tcataatgtt agcaataatt ttttttataa
    42301 taatgttaac ttatttatca aattcagtag tggggttgat aacaagttca ataattatat
    42361 ttatgtatac atcctatatt ttatataaca atattactca caatatggat gttgaattta
    42421 tatcatatat gtggataata gcaacaccag tatcttcaat tataatgggt aatctgaaca
    42481 aaagcataaa tgaattgcaa aatacaaata aaaaactatc tgaacagtat aaggaattag
    42541 tgactattga tagtgaaact ggacttagaa atttaaagat tttttataat gatgtaaata
    42601 tggagataag taaatctatt agacataata ctgatttttc tcttatgata gtaaaattac
    42661 catactacgg aaacctacag actatatttg gtgaaaacaa aactaataaa atagtaaaat
    42721 atattggttc aaatattata gaatgtacaa gaaatgaaga tataatttat agtttacaaa
    42781 aagatatgat agggatacta atgccaaata catctttgga aggttcaaaa gttgttaaag
    42841 acagaataaa gaaaaggata aaagaattaa atttagattt aaacaatagg ggaaaatatg
    42901 taaatataga tgtcaaaatt gcatttttac aatataagag tagttttgga gatagtataa
    42961 attttaagaa tatagttgaa gaggaacttc aatatgatgt ttaaaatatt taataaaagt
    43021 ctattaaaat tagtagttgt attatcaata gtactgacaa tatcaaattt tagcttatct
    43081 tcatatgcat cagaaattac ttccagcaca gataaggtat taatcatata tgattctaag
    43141 aaagaaacag cttataatag agatatttta aatattatga ggactctatt gggaagattt
    43201 agttcagata tagagctatt aaaattgagt aactatgatg gtgagataaa taaaaattat
    43261 tacagtcata tatttatttt aggaatcaat gaaaactcat ataataatga taaaaacact
    43321 aaaaacttga ttagttcctt aaattcatat aaaggaacta tatgttggtt aggatatggt
    43381 atagaaaatc ttttggaaca taaaaaatac aatttagact atgtaggaaa aacaaacaat
    43441 atagttagtg taaattatag aggtaaatca tataacttgg atgaacatta tgtttttaac
    43501 attgtagaat caaaagatac aagtaataaa gtaataggaa gtattaatga taccttaaat
    43561 aagtatccat atataataaa tgataaaaat cttttttatg tatcaaaatt agatttagat
    43621 ggagttttat tttatatatt ttgcgattca cttaatgata tttttaatat taagacgttt
    43681 gataaaggac gtatatttgt aagaatagaa gatgtacatg cttttagaga accaaaaaat
    43741 cttgtagaga tagcggatta tttaagtagt aagaatatac catttacgat tgcattaata
    43801 ccagcatatg taaatcctaa aaatcataag gtaataactt tatcagaaag ccctgaaatt
    43861 gtcaaagcaa taaaatatat gcaagataaa ggtggaactg tgatattgca tggatatact
    43921 catcaataca agaaagaaga agtttctgga gaagggtatg aattttggga tggtaaaaaa
    43981 gatgaaccct taaaagaaaa tatgaagata tttgtcaaag atagagtttt gaatggtctt
    44041 agagtatgta tagagaatgg aatttatcct cttgcatttg aagcgccaca ttatgcaatg
    44101 gaatcagagg gatataaaga attaaaaaaa tatttttcta cttatatggg gcaacatcaa
    44161 aataatgata aaaaatttag cacaaataca tatccttata ttattaggga tacagaggag
    44221 tttaatatat ttatacccga aaatttagga tatatagacc cagaagataa gtttactttt
    44281 caaaatataa aagaaaattt aaataaatta tccattgtaa gaggttttag tggagggttc
    44341 ttctttcatt catatttaaa catagagtat ctaaaaaaca caatagaata tttagaaaaa
    44401 cagaatatag aatttatgaa tttaagagat tttaataatt gggtaaaagt tgatgaaata
    44461 caaataagaa ataatggaga tgaaattata gtcaactatg ataaggactt agatgaaata
    44521 actaaaagtg atactagatt taaatccata tccaatatta gtaaagtttt aatattcata
    44581 gtatctataa gtgttttaat atttgtgatt atatttatat attttaagag aatagataaa
    44641 aagaaatttt taaaataaat gattttatat tagaatcaat attttaaaat attttaggag
    44701 aaatttatga gagatttaaa tgtatcagag tatttgttcg tatttagtct gtttagtata
    44761 tggtctttat tgcttataaa tattatttta gctatgggtg gatatatatt ttattttaaa
    44821 aactttgata aagaaataaa agaaattgat gaatatccta tgatatcaat acttgttcct
    44881 gctcataatg aagctaaagt gataggtaga actgtggagt ctttgctatt actcaattat
    44941 cctaagagta agatggaact tattgttata aatgacaatt cttctgataa tagtaaagaa
    45001 attttagaaa atataaagga taggtacaac aattataatt tcactataat aaatacagat
    45061 agtttaacag gtggaaaagg aaagtctaat gcattaaata ttggatatac gatttcaaaa
    45121 ggagatttta tagctgttta tgatgcagat aacacaccag ataaaaatgc acttagatat
    45181 ctagtacaaa ctatagtaat gaatgatgaa ttaggtgctg ttattgggaa atttaggact
    45241 cgaaataaaa ataaaaacct attaaccaaa tttataaaca tagaaacatt aagctttcaa
    45301 tggatgtcac aagctggtag atggcaactt tttaatctat gtacaatacc aggaactaac
    45361 tttatattga ggagaagtat catagaagaa attggaggat gggatagtaa agcaattgca
    45421 gaagatacag aaataagttt taggatatat aaattgggat acaagataaa gcttgtacca
    45481 caatctataa catgggaaca agagccagaa actgttaagg tgtggataaa gcaacgtact
    45541 agatgggcaa aagggaatat atatgtatta atgaaatata taaaaaatat atttaaacag
    45601 ggaagaaata agattgtgtt tgatatagct tacttttttt ctgtatattt tctattttta
    45661 acatctgtaa ttatatccga tattttattt gttttaagta tatctaaact agtagaaatt
    45721 tctattccaa ttaacttttt tctaatatgg atactatctt atcttttatt tattatagaa
    45781 gtgagtataa gccttactat agaaaaaggt gaagcaacaa ttgaaaatat attcatagtg
    45841 gcaataatgt attttacata ctctcaacta tggctgtttg tagctataaa aggaatgata
    45901 gagtatctaa aagatataat ttttaaaaga gaggttaaat ggtataaaac agaaagattt
    45961 tgattcatgt acaggaggaa ttaacatgaa aaaatttatt atatcaatta tatctttagt
    46021 attgtttttt tcaaatatat cacttatata caaagtaaat gctgatgaga ctaaagttaa
    46081 aaattataag tttgaaagag atataactat tgatggtgtt ataggaagta atagtacttt
    46141 ttttgaagta aataaaaatt gggatattga agaagtttta ttgcatctta attttagtaa
    46201 gagccaaata ttaaatggag atgtttcaag tttaactgta ttgataaata atgtaccaat
    46261 aaaatctatt aaattaaatg ctaagacaaa ttataaaaat acattagaag tattagttcc
    46321 aaaagattat ataattcaag gatataatga aataaaaata aaaacataca aaacaatatc
    46381 tgataaaatt tgccaagatg attccaatac tgggaactgg atggttattc acaaagaatc
    46441 atatatatca attagatata agcagaaaaa ggtggaaaac tcaataaatg aatatccgta
    46501 tccatatgca gaaattgaaa ataatcataa attagataca actatagtag taccagataa
    46561 tatgactaga ggagagacta cagcagtgtt taatttggca tcagcatttg gaaaaataac
    46621 aaaaaatgat gatttaaagt tagatgttaa attatattca gaaatgaaaa attggtcaga
    46681 tgataatatt atatatattg gtaaacctga aaatacagca gaagaaattt tggatatttt
    46741 aagtataaag gaacaaaccc tattatcttc aaactgtata atcaagcaag tagattcacc
    46801 atacaataag aataagaaga tgatggtggt aattggtagt aatgaagatg atttaataaa
    46861 ggcatcaaat ttattaatag aaaatagact ttctaaccaa gttttatcat catcagtact
    46921 tgtaaacaaa gagactaata taaaaattaa tagggaacaa aaattaaatt taggtcattt
    46981 aacattaaaa gacttaggat attctgactt tttgttggaa ggagcattta atcaacaagc
    47041 tttgtttgat gtcaaaatac caacgggtaa ggtattagat gatggttcaa agattatctt
    47101 aaatttaaga tattctgaca atttagattt tgaaaaatct ttagttacag tgtctataaa
    47161 tgatgttatt gtgggaagta aaaaattgga taggtcacat tctaataatg ataagttaga
    47221 attaaaaata cctaaagata tagataataa aaactattat caagttaaat tgacatttaa
    47281 tttgagtata aaaaattcga attgtgttac tagagagagt aataatcctt gggcatatgt
    47341 atcaaataat tcttatttgg cgttatctat gaaagaaaat gaaaccttat cttttgaaaa
    47401 ttatccgtat ccatttgtta gagatgatga atttaatgat ttaactgtga taatgccaga
    47461 ttattcaggg tcacaagcta tgacttggat gtttagatta ggtgttactt taggtgcgaa
    47521 tataaattca cataatggaa atataaatgt aattagagga aaagaattta gtgacaagta
    47581 taaggatact aatattgtag tttttggagt gccacataat aattctgtta taaagatgtt
    47641 aaataataat ttaaatataa aattcgataa aaattactcc aattttatat caaatgataa
    47701 gataagcttt attgatgatt atggaaaaaa tatttctacc attcaattaa taaaatcacc
    47761 atataataat caaaaaaata tcatggtaat tagttcaatg aatgaaaaaa acttatattt
    47821 aggaatggat tatttgttga ataaaagtaa agttaatgat ttaaaaggag atacattgat
    47881 aattgatgaa tacggagagg tagaagattt agcttataat ttaaaatcta agaaagaagt
    47941 aaaagattca agttggaata tgagtataaa taagacaact aaagtatttt taatgatttc
    48001 ttttattact ataattgtag tcatgatatt atcaatgcta tatataaaaa aatataaaag
    48061 aagataatat gataaattta ttattttaaa agaggtatat aatttgagga aatatatgaa
    48121 taaatttaaa ttaaaaagaa gtattttaat ctcattaata atcttaattt ttatatttat
    48181 agtttacttt gtaagtattg agttaaaaga taggagtcta gtaaataatc aatataaagg
    48241 taagattaaa tcggccaatt tatctgtaga ttatgaaata aatcaagtta tgaaggatat
    48301 agataaattg ggattgaata cagtaaatgt acctataatt ataaatgtag agtccattaa
    48361 ttctgatatt atgtcaatag ataataatag taaagaaaag gcaattaagt taataaaaaa
    48421 attaaataaa aaaggtattt caacaatatt agaggcatat ccatggattg aaaatggaaa
    48481 attgtatgag actgactgga accctataaa taaaaaaaga tttttttata cttggcaaaa
    48541 tgccatatta aatgaactta taattgatgt agcaaatcca ctggatgtaa atgttttaaa
    48601 tattggttct aattttgtac atttggaaga atatcaacaa aattggggtg aaataataga
    48661 ttttgtacaa tccaaatttg acggttttgt aacgtacaga actaattggt ggtatacaaa
    48721 aaaagatgat ttaagtagta agttgtttta tgaacaaaag ctgaacaata atttttttga
    48781 taagttagat tttatatcta tagcagcata ttttgagtta agtaataaac ctgttaatac
    48841 agtggatgaa ttaatttctg ccttacactc atctactgta aacaatagag gacaaaatat
    48901 aaaacaagaa atatataacc tttataaagc tcatagaaaa cctatatttt ttggagaatt
    48961 aggtttttca aatagagaaa gtgcaagttc tcagccgtgg aatcatactc cttcaaaagc
    49021 tgtaaatggc gaagaacaag caaggtgttt tgaggcatat aagaaagttt ttgaaaatga
    49081 agactggata aatggttttt ctgtgttttg tgtaggtaaa atagatgacg aaaaaaattt
    49141 ttacccatca aaagagagta taaaagttat taagtcttgg tatgaataat tttgaaagtg
    49201 tagaatttat atagattaaa tagatgatta ggagagacat aaatgaatga catcaaggtt
    49261 atgactgtat ttggaactag acctgaagct atcaaagtag cacctttaat aaaggaactt
    49321 gaaaaaagag agaatataaa aagtattgtt tgcgttacag ctcaacatag agaaatgctt
    49381 gaccaagtta tagaaacctt taatataaat gtagactatg atttggatat aatggaaaaa
    49441 ggacaaagcc tgaatgatat aacatgtaag attttaaata aacttccctt gattttaaac
    49501 aaggaaaatc caaatataat attggtacat ggagatacaa ccacaactct agctacaagt
    49561 ttgactgcat tttataataa aactcttgtt ggtcatatag aagctgggct tagaacctat
    49621 gataaatatt ctccttttcc agaggagtta aatagacaac ttacaggtat tattgctgat
    49681 atgcactttg ctcctactaa tttggctaga aaaaacttga tttctgaagg aaaacctaat
    49741 aataatatat ttgtaactgg taatactgct attgatgctt taaagatgac aataaaagaa
    49801 aactataatc atcctataat tgatgagatt ggtaacgata ggatgatttt actaacctct
    49861 cacagaagag aaaatttggg taaacctatg aaaaatatat ttagagctat aaagcgaata
    49921 gtagatgact ttgaagatgt acagatagta tatcccatac atttaaatcc taaaatacga
    49981 actatagctg atgagatatt tggaaaattt cctgaaaaaa ttcatattat agagccttta
    50041 gatgtagctg attttcataa ctttttaaat aaatcttata tgataatgac tgatagtgga
    50101 ggaatgcagg aagaagctcc atcattaggt aagccagtcc ttgtgttgcg tgataaaaca
    50161 gaaagaacag aaggaattga agcaaagaca cttaaattag taggtactaa tgaagataga
    50221 atatataatt cagtgagcga tttattaata aataaagata actatgttca gatgagtaaa
    50281 gcatctaatc catatggaga tggaaatgca agcaaatata tagtggatat aataattaaa
    50341 aaatttaatt gcaaatattt aaattagata agacctaaaa cattaactaa ttgatataag
    50401 atttatgata atattaagga atacaatttt taagggggag taatatggct agaaaattaa
    50461 taaaaaattt gggtaaaagt aaaagtgtaa agagagtgaa acttttattt aagaagattt
    50521 ttattactgt attcatagta gcaagtatag ttgctatttt taatataacg aaatattttg
    50581 aggaattata caaagtaagg gatttaaaaa gtactaaaat tgaatactat atggatgtgg
    50641 ctgatgaagc aggagatgga aaggtccaat taagttggaa ggccttactt gctatagaca
    50701 tggtaataca tgatgaagat ttaagtaata taaaaaagaa agacacattg gatatagggg
    50761 aaaagtttat agtagaagat aaaaatgata aaggcgaaaa ggtgtataag gtaaaaaagt
    50821 ttaataaggt attaagcgaa ttgaaatttg actcttctca aaaaagtaga gcaagaaaat
    50881 acatgaaaga tttagaatac acataccttg gaaataaaca attagatagt agtgatgaaa
    50941 aaattaaatt tataaagaag ttagaagact cagctataag agaatatatt gattatggaa
    51001 tattgccctc tataacaatt ggacaagcta tattagaatc tggttgggga aattctaaac
    51061 ttacaaaaca gagtaataat ttatttggta taaaagcaga taaagcatgg aaaggaaaaa
    51121 gtgtagaaat ttcaacttca gagcattata atgaaaaaat tgtagctagt tttaggtcgt
    51181 ataattcatt acaagattct gtcaaagacc acagtttatt tttaattaat aataaaagat
    51241 atagaaaaca tggattgttt gaggcaaaag attatattag tcaagctcaa tcattagaaa
    51301 atgcaggata tagtacagca gaagataaaa aaggaaatcg tatatatgca gagctattaa
    51361 tagatgttat taggagttat aatttgcaat tgatagataa taaagttgag acaaaataat
    51421 tgttaacttt atatgaggag ataacaagat aagattcttt tctacaaaaa gaatagaaaa
    51481 attgtaaaaa aaactaaaaa aatgtattga cttgttattt aaaaggtgat attattatta
    51541 ctgtactcga aagagaacaa aa
//
LOCUS       DAMJWS010000018        50716 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMJWS010000018 DAMJWS010000000
VERSION     DAMJWS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.018"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..1337)
                     /locus_tag="PC399_001126"
     CDS             complement(<1..1337)
                     /locus_tag="PC399_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2591999.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKKGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFVGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISR"
     gene            complement(1374..2594)
                     /locus_tag="PC399_001127"
     CDS             complement(1374..2594)
                     /locus_tag="PC399_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2592000.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAVVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            complement(2963..4975)
                     /locus_tag="PC399_001128"
     CDS             complement(2963..4975)
                     /locus_tag="PC399_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2592001.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            5446..6096
                     /locus_tag="PC399_001129"
     CDS             5446..6096
                     /locus_tag="PC399_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF2592002.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(6236..7612)
                     /locus_tag="PC399_001130"
     CDS             complement(6236..7612)
                     /locus_tag="PC399_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2592003.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(7628..9541)
                     /locus_tag="PC399_001131"
     CDS             complement(7628..9541)
                     /locus_tag="PC399_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2592004.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            9677..10309
                     /locus_tag="PC399_001132"
     CDS             9677..10309
                     /locus_tag="PC399_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF2592005.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            10448..10987
                     /locus_tag="PC399_001133"
     CDS             10448..10987
                     /locus_tag="PC399_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2592006.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(11163..11333)
                     /locus_tag="PC399_001134"
     CDS             complement(11163..11333)
                     /locus_tag="PC399_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2592007.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            11618..12685
                     /locus_tag="PC399_001135"
     CDS             11618..12685
                     /locus_tag="PC399_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF2592008.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            12855..14756
                     /gene="cooS"
                     /locus_tag="PC399_001136"
     CDS             12855..14756
                     /gene="cooS"
                     /locus_tag="PC399_001136"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2592009.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            14749..15222
                     /locus_tag="PC399_001137"
     CDS             14749..15222
                     /locus_tag="PC399_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2592010.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            15197..16423
                     /locus_tag="PC399_001138"
     CDS             15197..16423
                     /locus_tag="PC399_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2592011.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(16562..17749)
                     /locus_tag="PC399_001139"
     CDS             complement(16562..17749)
                     /locus_tag="PC399_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2592012.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            18022..18846
                     /locus_tag="PC399_001140"
     CDS             18022..18846
                     /locus_tag="PC399_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2592013.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            18975..20240
                     /gene="gluD"
                     /locus_tag="PC399_001141"
     CDS             18975..20240
                     /gene="gluD"
                     /locus_tag="PC399_001141"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF2592014.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            20453..21043
                     /locus_tag="PC399_001142"
     CDS             20453..21043
                     /locus_tag="PC399_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2592015.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            21373..22506
                     /locus_tag="PC399_001143"
     CDS             21373..22506
                     /locus_tag="PC399_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2592016.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGIEYYDSLLND"
     gene            23422..24444
                     /locus_tag="PC399_001144"
     CDS             23422..24444
                     /locus_tag="PC399_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2592017.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            24741..25661
                     /gene="pyrB"
                     /locus_tag="PC399_001145"
     CDS             24741..25661
                     /gene="pyrB"
                     /locus_tag="PC399_001145"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF2592018.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            25700..26392
                     /locus_tag="PC399_001146"
     CDS             25700..26392
                     /locus_tag="PC399_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF2592019.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            26385..27287
                     /locus_tag="PC399_001147"
     CDS             26385..27287
                     /locus_tag="PC399_001147"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2592020.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            27372..27956
                     /locus_tag="PC399_001148"
     CDS             27372..27956
                     /locus_tag="PC399_001148"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF2592021.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            28171..29097
                     /locus_tag="PC399_001149"
     CDS             28171..29097
                     /locus_tag="PC399_001149"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDF2592022.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(29149..30039)
                     /locus_tag="PC399_001150"
     CDS             complement(29149..30039)
                     /locus_tag="PC399_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2592023.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            30227..31258
                     /locus_tag="PC399_001151"
     CDS             30227..31258
                     /locus_tag="PC399_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF2592024.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            31552..32427
                     /locus_tag="PC399_001152"
     CDS             31552..32427
                     /locus_tag="PC399_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDF2592025.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            32445..33935
                     /gene="cls"
                     /locus_tag="PC399_001153"
     CDS             32445..33935
                     /gene="cls"
                     /locus_tag="PC399_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2592026.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            34128..34412
                     /locus_tag="PC399_001154"
     CDS             34128..34412
                     /locus_tag="PC399_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF2592027.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            34445..36073
                     /gene="groL"
                     /locus_tag="PC399_001155"
     CDS             34445..36073
                     /gene="groL"
                     /locus_tag="PC399_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF2592028.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDVGMPGMGGGMPGMM"
     gene            36335..37075
                     /gene="larB"
                     /locus_tag="PC399_001156"
     CDS             36335..37075
                     /gene="larB"
                     /locus_tag="PC399_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF2592029.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            37159..37893
                     /gene="larC"
                     /locus_tag="PC399_001157"
     CDS             37159..37893
                     /gene="larC"
                     /locus_tag="PC399_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF2592030.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            37923..38342
                     /locus_tag="PC399_001158"
     CDS             37923..38342
                     /locus_tag="PC399_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF2592031.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      38402..38503
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            38724..40259
                     /gene="guaA"
                     /locus_tag="PC399_001159"
     CDS             38724..40259
                     /gene="guaA"
                     /locus_tag="PC399_001159"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF2592032.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(40336..41382)
                     /locus_tag="PC399_001160"
     CDS             complement(40336..41382)
                     /locus_tag="PC399_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2592033.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(41459..41674)
                     /locus_tag="PC399_001161"
     CDS             complement(41459..41674)
                     /locus_tag="PC399_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2592034.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            complement(42108..42509)
                     /locus_tag="PC399_001162"
     CDS             complement(42108..42509)
                     /locus_tag="PC399_001162"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592035.1"
                     /translation="MQTLAEIIKEYRSEHNLSLREFSNLCGVSHTYIDKIEKNRDPRN
                     GKAVEPTLDMLEKMSFGLNLTLKELLTKLGKIQPNTQQDDELLKKVNSKEDNEVNELI
                     NRLASLDSDDKNAIKKMIDNAYYKAVNTKKE"
     gene            42674..42886
                     /locus_tag="PC399_001163"
     CDS             42674..42886
                     /locus_tag="PC399_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592036.1"
                     /translation="MKNNLQYIRKESRISQKKFADKIGISRPYLSRIENGKVNPSLEI
                     AHKISLETGKTINEIFFDFTVNHSKL"
     gene            complement(43181..43399)
                     /locus_tag="PC399_001164"
     CDS             complement(43181..43399)
                     /locus_tag="PC399_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592037.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYESGSREPN
                     IETLILISNKLNVSTDFLLGLSPNQNRN"
     gene            43518..43706
                     /locus_tag="PC399_001165"
     CDS             43518..43706
                     /locus_tag="PC399_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592038.1"
                     /translation="MNNNLKLQREKVGLTQLEVAQKAKITERSYQYYEAGERLPNIRT
                     ALKIARILNTNCEKLFNE"
     gene            43748..44542
                     /locus_tag="PC399_001166"
     CDS             43748..44542
                     /locus_tag="PC399_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDF2592039.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEINHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSAYLDLKNENRKCYNVTKLGCDFLANKFTGEKGIIFTARYVKRF
                     NKMEQELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADS
                     VASSDNSILVGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLG
                     VIETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKKNNQISMLG"
     gene            44626..45006
                     /locus_tag="PC399_001167"
     CDS             44626..45006
                     /locus_tag="PC399_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592040.1"
                     /translation="MKNNTSDSRVKYFCKCPYCGFDNEVEVKKGSKPKICCICTKEVE
                     YEKLEQQSVLENTEIKEVCNQMKIKKLALATGEVFENIELKEFENKGECLPPNMVKVI
                     DGKQELLINKEFILSLEVKRKTTF"
     gene            complement(44996..45148)
                     /locus_tag="PC399_001168"
     CDS             complement(44996..45148)
                     /locus_tag="PC399_001168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592041.1"
                     /translation="MVIPIAKNTKQTSKTVASKASKILKDGRYSAASKSVAGSALSQT
                     KKGPKK"
     gene            45207..45404
                     /locus_tag="PC399_001169"
     CDS             45207..45404
                     /locus_tag="PC399_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592042.1"
                     /translation="MAINDNINKILRDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKLADYLDVTLDELVGR"
     gene            45443..45649
                     /locus_tag="PC399_001170"
     CDS             45443..45649
                     /locus_tag="PC399_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592043.1"
                     /translation="MAKAVAKEQLFYRAKDIAKILDVCEATAYKIIGELNKELEKEGW
                     KTFPGRVSVTYFKERYCYKTKKGA"
     gene            46062..46379
                     /locus_tag="PC399_001171"
     CDS             46062..46379
                     /locus_tag="PC399_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592044.1"
                     /translation="MKSLIIVRNSVEQQLNRANLEINKNEQLYTKLRKKEERNISEEI
                     ELSNALREKSVNERLKIFAESLLKIIDTQIEIKEYEESEDYKIFELISEELERDIPID
                     VQI"
     gene            46462..46971
                     /locus_tag="PC399_001172"
     CDS             46462..46971
                     /locus_tag="PC399_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDF2592045.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRAKKNTLDKLKSNIKDCMELLETKKVETIL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            46981..47589
                     /locus_tag="PC399_001173"
     CDS             46981..47589
                     /locus_tag="PC399_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF2592046.1"
                     /translation="METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVVLDDNIVQIGNRFYVEATATLIDAETGEKISVKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNILYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            47599..48480
                     /locus_tag="PC399_001174"
     CDS             47599..48480
                     /locus_tag="PC399_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2592047.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLYKRSKNGRDATANILKELIENGYVTRKPCRDSNTNKMLGGYDYQVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYYESYIGVITPNNFLQLLSYLDDGMEADVIIRAVDEAVGSGVKNYKYIKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKSKQEKKQSNNKTVNTHNVSKNKFANFNQTFTQYD
                     EKELDEIIKKSQKEKFK"
     gene            48541..48714
                     /locus_tag="PC399_001175"
     CDS             48541..48714
                     /locus_tag="PC399_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDF2592048.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKIGREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            48730..49146
                     /gene="ssb"
                     /locus_tag="PC399_001176"
     CDS             48730..49146
                     /gene="ssb"
                     /locus_tag="PC399_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2592049.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKILFRVAVDRKYKDREGKK
                     KVDFIPCEQLGKHVENLVQYLTKGKQIYAFGELNIDNYKDENGCWKSITKVNVNALEL
                     LSSKEDSNNHEKQQEYIPPGLDPQGFQAIDDDDIPF"
     gene            49217..49630
                     /locus_tag="PC399_001177"
     CDS             49217..49630
                     /locus_tag="PC399_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592050.1"
                     /translation="MELKFREWNKNGKEMYSYDEMVCYSKNLLREWVYSGVYLPKNNE
                     NFEVMIYTGLKDCDGKEIYEGDIVLCRSIFFTEFAGEVKFKDGCFIAVNEMSGDCFRL
                     SELAIVKVDGNIYEDLSKLNDIRRCDEGGIRNDNN"
     gene            49617..49868
                     /locus_tag="PC399_001178"
     CDS             49617..49868
                     /locus_tag="PC399_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592051.1"
                     /translation="MIIIRSQDRLDLMRVNRVEIGFNQVYAILENDVRKIGEYESNER
                     AMQVLNEIQKFIENGVRTDYIDSCRVRHNQEKVFEMPFE"
     gene            49886..50239
                     /locus_tag="PC399_001179"
     CDS             49886..50239
                     /locus_tag="PC399_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592052.1"
                     /translation="MAKVWMDAGELLEKTIDIEDMFGCNLSKMRNKSKQKGLVGRTNS
                     KGQGRKSKKVECTNIITGESKIFVSAVEASKYLYFTELHIARLARTGKTTKNGWKVRY
                     IQEVTDGIGKCGASN"
     gene            50464..50640
                     /locus_tag="PC399_001180"
     CDS             50464..50640
                     /locus_tag="PC399_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592053.1"
                     /translation="MDDRLEIINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
BASE COUNT        19703 a         5721 c         8529 g        16763 t
ORIGIN      
        1 tctctcgaaa tatataattt agaatcttct gatttatttt tatgctcgat ttccttattt
       61 tttttcataa atatatatag aggagctatt aacaacattg cccctaatcc tattaaaact
      121 ctattttgag ttataggttt tgaaatatct tctgcccaag cttgttgtaa caaccataag
      181 ctacctaaaa tagctactat tggtataaca gggccaaatg gaatcttaaa agaggcctta
      241 acatctgttc tttttcttaa aactattact gataaacaag ttggtatgta ctgagcaaat
      301 cttgcaatta cgctcattac tgcaagttgt tcaaaacttc cagttagcac aagtggtata
      361 caacaaatta aagatattat tattgctaca tatggagcac catatttatt tgtttttcct
      421 ataaatgcag gaagagaacc ttcttctgct agagaagaac cacatttagg agttactatt
      481 gatgaaccta tatttatacc aaatatagat attaatgttg ctacagatat gaaaacttta
      541 ccataattgc ctacaaatac acttgaagca tctgcaattg gaatactgtt ttcaaatagt
      601 ctatctccaa gtatacccat acagacaatt tgaattaata tataaattat tgcacatata
      661 aatattgtgg ttattaatgc gactggaaga ttttttttag gattttccat ttctccactt
      721 gccacaataa atgattcaaa gccggtaaat gcgtaaaata ctaatataat ggctggaccc
      781 atactagagt taactacatt tcctgaagga acaatattta caaattttat aaaaaatatt
      841 ccaactatta taaaaactat taatggtact aatttagcta tagttattac attattcatt
      901 atctttgtgc ttttaattcc aaataagcta tttattgaaa gtagtgttac gagtattgca
      961 gctatataac ctttgtattc agtggcagat tctggccaaa atgaacctaa agctgttgca
     1021 aatcctactg ctagagtaga ccatgatata attctaataa cccaagacat tgtaccaact
     1081 tcaaatccta taaaatctcc aaaagctttt ttggagtata gatatgctcc tcctttctta
     1141 tcaaacatgc ttccaacttc tgcaaaacat aaaagtattg atagaactaa gagtgtagca
     1201 aaaatgtata taaacatact gttttgacct gctagattat aaactttacc tggtagtaag
     1261 aatatacctg aacctattat gctattaatc cctaataata tcatactaaa tagacctaat
     1321 ttcttagctt tttccatatt aatttatctc ctattctttt attaattact gtactaatta
     1381 aaatacaaag tattcattac atgcatcata acacctattg gtaaacaatc ttcatctata
     1441 ttaaagctag aggtatgaag aggtgaaatt aagccctttt cctcattttt acatcctaaa
     1501 tggaagaatg ctcctttaca atgttctgtg taaaaagaaa agtcttcacc acctaaagac
     1561 gggtttggtc tcaatataaa tttttcctct ccaagtaatt ctttagtatt tgatataact
     1621 gtatctacta attctttttc atttataaca gcaggatagt tttcatcact tacatgtaaa
     1681 gtcccaacac aaccaaatgc agatgcagta tctccaacaa ttttagctat tttatcaatc
     1741 ataaagtttc ttgttttgct atttaaggtt cttaaagtgc cttctaattt tacatcttca
     1801 catattacat tgtgtgcatc accaccatat attttaccta tagtcaaaac aacagcatta
     1861 tttggttcta agtttctact tattatagtt tgtaatgatg taacaatttg tgaagctgta
     1921 actattgcat caataccgtt ttcaggatat gcaccatgag ctcttttacc tttgatactt
     1981 atttttatag tgtctacact agcatttaat gtatcatatt ttgtttcgat aaggcctgtt
     2041 tctatatgtg gcataacatg aagtccgaat atataatcgg ccttggggtt ttctaaacac
     2101 ccatcctcaa ctaaaaattt tgctcctcca aaaccttctt ctgctggttg aaataagaat
     2161 tttacattta cattaagttt atcttttatt gaatgtaaaa ctttacatgc tccaagtagc
     2221 atggttgtat gtgcatcatg gccacaagca tgcatttttc cagaatgtat tgatttataa
     2281 ggtatgttgt tttcttcttc aataggaaga gcatccatat ctgctcttat acaaatcgtt
     2341 ttatctgcac tttcttttaa aatatatgcc ataattccat tgtgttttgt aaattccata
     2401 taatcaattc cgatttcttt taaataagaa attaccttct gtttagtaag attttcttca
     2461 agagctaatt ctggaatttt atgtaattct cttcttatgt ttataagcca tggttttata
     2521 gaattacaac tttctattac aaaatctttt cctaattctt tggtttgata ttttaatttc
     2581 aaagcctgtt gcatttttat tcctccaatt gctttttatt gttttattaa tataataagc
     2641 aattattatg ccataatttt cgatggagaa ttttgttttt tataagaata ttgaataaat
     2701 ataatcattt atctatatgt atagtataaa aaatgattgc atagtttcaa ttttcagtaa
     2761 aaaatattat tttgaagaaa atatatttta tatgctagtt tatataaaaa aaataggaaa
     2821 taaaaaaatt atcctattcc tattaattcc tattatttat gtacaaaata aattataatt
     2881 ttagggtact tgaaaaataa aaaaacctat tgaaggagta ctttaatcct gaatagattt
     2941 ctttgtttga taaattataa ttttatagac ctttatattt taatttattg taaaaatctt
     3001 ctcctttttg agttatttta cttccatatc ttcctgaact acaaattata agttcttctt
     3061 tttttagata ttcaaatatt tttttaattt ttccttctgt aatttgaagg ttcttatcga
     3121 gtagtgcttt taaaattaat cctctaccaa taccaacatc tgattcctta tttaaaatta
     3181 gtatctctaa tatattcaat atatcattct tactatattt tagttttaat gatgtatttt
     3241 tgttatctga agaccttaag tttggaggta aatctttctc taatacaata tcttcacaca
     3301 taatgtttaa ataggatgta acattttgga gttctcttat attacctttc cacatatagt
     3361 tttgtataag tttttttgct tctggtgtga ttactaattt cttattaatt aaatcttcca
     3421 taataattaa tatgtcttct tttctttctc ttagaggagg tatatttatt ggaattgtat
     3481 ttaatcgata atataaatcc tctctaaatt ggctattatc aatcatttgt tctaaattct
     3541 tatttgtagc agatatgatt ctaacatcta tattaattac attgtgagat ccaacaggca
     3601 ttatctgctt ttcttgaaga actctaagta gttttgtttg taattctaaa ggcatatcgc
     3661 ctatttcatc taagaatatt gttccattat tagcaagttc aaataatcct tgttttccat
     3721 cttttagacc tccagtaaaa gttcccttgt catatccgaa tagctggctt tctaataaat
     3781 tttcaggaac ggctgcacaa tttactgcta taaagggttg attttttcta tttgagttgt
     3841 tatgtataga ctgagctatg agttctttac cagttccact ttcacctctg ataagtatgc
     3901 ttaaatcaga tttagaaacc ttcttagcta aatctataca ttcaaacatt ttaggactat
     3961 ttgtttttat atccttaaat gtatattttg ctatttgtcc tttttctctt aattttttag
     4021 tgagattttg ttctaatttt tttatgtatg ttatttcttg taaactataa tatgtgccca
     4081 ttttttcatc tctattgtat atgttttttt tatttacatt tatatatttc ttattgaata
     4141 cgacaacttt atctagtatc tctttctcac tcaataaaac ttttaaacta tctacaaata
     4201 tatcctcaat gtattttcca tatatatcct catttatgtc taaaatatct tttactttac
     4261 tattatatgt atttatttca ccatcttttg aagtaaataa tataccatct tttgataaat
     4321 tcaagattgt atctaattct tctattttta aaaataattc cttatattta tcttttatac
     4381 cagtatctaa acttattatt tcttctgagt atttaactag attagattgt atttcctttg
     4441 aatcaatttg taataaattt attatttcta taaatgtaga gatatctata tatctgtgac
     4501 ctaaatcaaa tatatctgag ataaagctag gtactttttc tggaactcct ggagttatag
     4561 ctatttttat atttttatag ttattatcat tcatatatgg aattggtcta atattagtca
     4621 ctccaatttt ataaaataaa cttatagttt ctagagtagt ttcatcagaa tcatttacga
     4681 ttagaacatt tgtaccctgt ggtaaagata gtagattata aattttatct tccctaaaag
     4741 ttcttcttac cacaatcact tttttcttat ctagtatatt atttataatt ttgtctaatc
     4801 tttctactga cattactaat atcacatcat catttataat atcattatca tttatttcat
     4861 ttatgaagta tctattaata tttatagaat ctccaaaaac taatttcaaa ttattttcta
     4921 aaaagtctat taaatctttt ctactatcat ttaccagagc tactgatttt ttcatttatt
     4981 tctccttata taataaatag tttccaacaa aatagttgta taaagcaatc taattttgat
     5041 aatttaaatt tatcaaataa aaaagaaagt gtctataatg actttctaaa agttgcatta
     5101 agacactttc tttcttatag ttaaaaatct tgtcaccaaa tccccctaat tgttgcttaa
     5161 aacttaaact gtatgtatat ttttcatttc tattcgatta aattataata tacacccaaa
     5221 aaaatttatt ttaataaatt ctattgataa tataatttta taaatataga attatattaa
     5281 aataaattat atatgaaaat atatatgtat aagtttattc tgcgtgagta gaatggattt
     5341 gtcaagttta atttagttgt aattctatct tttattaaaa ataacataaa ttaaaacata
     5401 taaataaaat attttataca atatattgta aaggaggtaa ctttgatgaa agcagaaaaa
     5461 acaagtatat atccaattat taaaaataat ttaaacaaaa tatcaattgt tgaatataaa
     5521 aagggtcaaa gatttgtagc atcagataaa gacttacaag aagtgttctt tataatagag
     5581 ggagttgcat tagtggagtg tactactaga ggaggaaata aatttcttgt tgacattgta
     5641 cctgaaaatg aatttatagg caaaattagt tacatatatg aacataattt gaaatgtgat
     5701 atatttgcaa agactaatat aaaattattt cgatttgaaa aaaatgtatt tgaagagttt
     5761 tatctcaaac cagattttat tgcactattt cacagaaaat gtacaagaag aatatatgaa
     5821 ttatataaaa ccagaatggt aagagaacta ttttcttgca cagaagttat tgcctattgt
     5881 atatcaaata aacaagaaaa taatgtctgc aatataaaag ctattcgtaa tttcagtgaa
     5941 atctattcaa ttagtagaaa aagtagatac cattctctgc taaagttaac tgaaaaggag
     6001 atagtaaaaa aaatgggtaa tacatatgaa attttaaact atgatgaatt acataaattg
     6061 gcatttgaag taaaagaatt tttggaagat gagtaaaaat tgttacttaa tcaaaataaa
     6121 gtataataat ttctgtaaaa aatagaaata gtctcactga aaatcaaaaa tactttagtt
     6181 attttcaatg agactgttta gaaatattgt atacttataa tgtatagtat aattattaat
     6241 caatttcctt tactatatta atagcccatt ttttagataa tgctctagga acaccaaata
     6301 taaacttaac cacttcttca ctataagtga gcaaaacgag tacaaatata ggtgcacctt
     6361 taaatgcagc tataaaagtt agaggaatag aagctaacca catacatcct aattctaaaa
     6421 atagagcata tttagtatct cctccacttc ttaaaatacc aattataacc aatgtattaa
     6481 aagatttaag agccattagt attcccatta taaagaatat cttagttata tcagaagcta
     6541 agctatcaga aacactaaac atttttaata ataatggtat atttaaaatt aaaataattc
     6601 caagtattaa accagctgaa aatacaagta tagaaaactt tttagcatat tcaatggctc
     6661 ttttaatatg gtcagctcca agttcatttc caagcataat agatgcacca gatgcaatac
     6721 atacagctgt cattatgaag aaattacctg tactggttgc tatctggcta gctgctatag
     6781 cagaagtacc agctcttgca tatgcaacag aatacaggac agttccaatt gcccatacag
     6841 agtcatttac aaatatagga aagctttttg atgaaaaaga ttttataaaa attttatcaa
     6901 tagcttttag attttttaga ccaaacttta atatatagtc ttttttataa aggtaaacat
     6961 aagtaatcat taaaattaat tcacatattc tagcaataac agtagctaga gcagcacctt
     7021 ttacacccaa tgcaggaaga ccgaagttac caaatataaa tccataattt aaaataacat
     7081 ttgttacaag tgcaaatgca ctacaaatca tacctaactt aggatttcta acacctcttg
     7141 aaccagtact aaatacagta cttacagcaa ttaatggata actaaatact gcaatactaa
     7201 agtaatctat acaaagtttt accacctcag aatcatacga aaatatgtgt ataattggct
     7261 ttggtgttag taaagcagga ataacaaata caaaacttaa aacaattgcc aatacacaag
     7321 taagacctgt gacttttctt atattactca catccttctt tccataaaac tgtgaaataa
     7381 aaactccagc acctcctgtg ataccagata aggacatatt aaaaagaaag aaaaattggt
     7441 ttgcaactcc aactgaagct acagatgttt cccccaaact acttatcata attgtatcta
     7501 ttacatttac agaagtagaa attaagttct gtatgataat aggtatacat aaagatagaa
     7561 gtattttata aaatctttta ttttcgtctt ttaaagaaaa tagttcttgc aaataatcct
     7621 ctccttttta cataaattct tcccactcat catatagaga ggcaaggtta ttttctaaat
     7681 taattttttc ttggctaact tctttagctt tatctggatt agagtatact tcttcttgac
     7741 ataaaagtat atctaatttt tcaatttcaa tctctaactt ttcaatttct ttttctatat
     7801 tttgtctttt tattctattt ttcttttcta tttctctttg ttctctctct tttctttttt
     7861 catcttttat ttgagttcta gttttttctt ctttttcttc tataatactc atctcttgga
     7921 tttgcctttt tttatctata taataatcat aatttcccag atattcagta attccatttt
     7981 catctaaaac taaaatttta tcaacaacag tatttagaaa gtatctatca tgtgatattg
     8041 taaatatagt cccatcataa ttagttaagg cctcttcaag gacttcctta gaatcaatat
     8101 ctaggtgatt agtaggctca tctaataaca ataagtttgc atttgatagt ataagtttaa
     8161 gtatggcaac tctagctctt tctccaccac tcaatgtaga aatcttctta aaaacttctt
     8221 catctacaaa taaaaatgcc cctaacatat ttctcaaagt agtttgagta agatgagtat
     8281 tattttccca aatctcatca attattgtgt tatctaaatt aagagttttt tgttcctgat
     8341 ggaagtagga aacatgtaca ttagttccaa atttaatatt gccactaaga ggttgtagtt
     8401 cattagttat tattttaaat aatgtagatt tgccaacacc atttgcacct ataagtgcaa
     8461 ctttttcccc tctatatata tctaaatcca agtctttaaa aagtattctt tctccataac
     8521 ccatagatac atctctaatt tgaagaacat cattaccact ctggacagaa ggcgtaaatt
     8581 gtatacgagc ttttttccta taagcttctg gtttatctaa aacatctact ttgtctaatg
     8641 ctttttcttt acttcttgca cgctttaggt gtttttctct accataagct tttagtcttt
     8701 caattgattc ttcttgcttt tttatttctt tttgttggtc ttcaaatttc ttaagctcta
     8761 gttctttttc tattttagat aactcaacaa atttagagta gttaccatta tatactttaa
     8821 gctttttatt gtgaacttca aaaattctat ttacggattg gtctaagaaa tatctatcat
     8881 gagatattaa catgacagtc cctttatatt gttttaaaaa tacttctaac cattctattg
     8941 cttcagagtc taagtggtta gtaggttcat caagtaacaa tagagttggt ttcttaagaa
     9001 gtaattttcc taaaagaact ctagtttttt caccaccaga aagtatgctt attggcttat
     9061 ccatatcatt gtcactaaat cctaatccct ttagaactcc tttggcttca gatttatatc
     9121 catatccatt taaatctgaa aataattcta gtttatgtga atattcatcc atgatttttt
     9181 ctataattgg agagtttgtt tttgtacttt cttcagatat tttaacctcc aattctctta
     9241 aataggattc catttctatc aaatttttaa aaacttctag tacttcttcc aaaatagtat
     9301 tatccgaata aaaattggta ttttgttcta aatatccgat ttcacaatct ttagaagtgt
     9361 aaatatctcc actatcatat ccataaattc ctgaaattat tttaaataaa gtagtttttc
     9421 cagtaccatt aacaccgatt ataccaattt tatcgccttc atttactgta aaactaatat
     9481 tttctaatat agaatctata ccaaaacttt tatttaagtt attacatgac aaaacaatca
     9541 taaatttcta cctccttgaa ctatctaaaa ttataatgtt ttgatataat ataatcaagg
     9601 ttatatgtat tagttttgaa aaagaaatat tttattgtga atcaaacaaa aaagaggaca
     9661 cttttaggag gtgaagatgt tgggaaataa aaatatatca atggcagtta taagaaggct
     9721 cccaaaatat catagatatc ttggagactt attagatagg gatatacaaa gaatatcttc
     9781 taaagaattg agtgatataa tagggtttac cgcttctcaa ataagacaag atttaaacaa
     9841 ctttggtgga tttggacaac aaggatatgg ttataatgta gaagctcttc atactgagat
     9901 aggtaaaatt cttgggttgg atcgaccata caacgcagtt cttgtaggag caggtaactt
     9961 aggacaagct atagccaatt atgcaggatt tagaaaagct ggattcgaga taaaagcttt
    10021 atttgatgca aatcctagaa tgataggttt aaagataaga gagtttgaag tattagattc
    10081 agatacttta gaagacttta taaaaaacaa taatatagat attgctgtat tatgtatacc
    10141 taaaaatgga gcacaagaag ttattaatag agttgtaaaa gctggaatca aaggtgtatg
    10201 gaattttgca cctttagatt tagaagttcc gaaaggtgtt atagttgaaa atgtaaactt
    10261 aacagaaagt ttatttacct tatcgtattt aatgaaagaa ggaaagtaga caacttaaac
    10321 atattaataa tgagcagttg taaagagtat tgagaaagtc agaagattta atattaaatc
    10381 ctctggcttt ttgaaatatt aggataaaaa tttaaataat gtatggaaat aaacaatatt
    10441 aaggagagtg gtttttgtgg aggaattatg gaaaaaggca attgaatttc atggtcatga
    10501 atgtccaggg ttagctatag gatttaaagc agcacttgca gcaaaaaaat atttaggatg
    10561 taaacagtca ggagatgagg aaattgtatg tatttctgaa aatgatgctt gtggaataga
    10621 tggtatacaa gtagtgctaa gttgcacaat gggtaaggga aatttactac ttaggatgac
    10681 tggaaaatca gtatatcatt tctttaatag agaaaatgga aaatctataa ggatatatgt
    10741 aaagtcaaaa ccatttgaga tggatagaga aggatacaaa agatatttat tagaaagtga
    10801 ttttgaagaa atatttgaga ttacagatac taagttaaga cttcctgaaa aagcaagaat
    10861 atttaaatcc cttaaatgtg aagtgtgtgg tgaatttgca tctgagcaat atataaggat
    10921 tgaagaagga aagaaagtat gtcttgattg ttttgataaa tatgaaagat tttatgaaga
    10981 gtattaaaat tataaattag attaaagtta aaagtattag atatgtgcta acagtatttt
    11041 aagatgaatt aggtagcaac ataataaagt atataaataa tattaattga atcatacata
    11101 attaaaaaga ggctgattag tatttataaa ataaaatcag tctctttatg aataacgaat
    11161 tattattctg gttgaggtgc atctacagga caagctcctg cacaagaacc acagtctata
    11221 caagaaccag catctataac atatgcgtcg tctccagctg atatacagct aacagggcat
    11281 tcagcctcac aagcaccaca gcttatacaa gcgtcagtaa ttttatatgc cattttgaaa
    11341 tcctcccttt tatatgtatt gtaattcaat aacattataa caaatattat tgaatataac
    11401 aattaaatta attctacaac aaaaacttta ttatgatatt cttgttttaa agttttacaa
    11461 taaagtttaa tctgcttata tttttattta ttttaaagaa gtttatttta aaaataaaat
    11521 taaatttaaa aagttatgta ttgattatat ttaaaaatgt tatactctaa aaaagtatat
    11581 tttatttaaa aatttattaa tacaaaggag atttactatg aaaaataaat tagtttacat
    11641 atgtattatg agtctattgc tatttgtagt aggatgttct aataatccaa ataaggaaag
    11701 taccattaaa aaagactcta agaataacca aagccaagtt aaagaagaaa aaagagaacc
    11761 tgtgaaaaaa gatataacta taagtttcgc tggtgatgta actatgggga actataaagg
    11821 ttcatcatat tatggcagtt ttgaccatga atttaaaaga caaggagaag attacaatta
    11881 ttttcttaaa aatgtaaaac ctgtatttga aaaagatgac ttaacagtgg ttaatttaga
    11941 aggtccactt actacagcgt caaatgcaaa agaaaaaaaa tttgcattta aaggaaagcc
    12001 tggatatgta aatattttaa aatctggaag tgtagaggca gtaagtattg caaataatca
    12061 ttcagaagat tattttgaag aaggaatgaa agatacacag tttatattgg acgagaataa
    12121 aataaattat tttggattag ggaaagatgc tgttgtagat gtcaaaggaa ttaaagtggg
    12181 tctattaggg tataatggat tatcaactga atataatgaa aaaaatttaa aacaaatgga
    12241 agatgatatt aagtccttaa aaaagaaatc ggatgttgtt gtagtgtact ttcattgggg
    12301 gatagaatta gattattatc cagataaaaa acaaaaagat tttgctcatt atgctataga
    12361 caagggagca gatttagtta tggggtctca cccacatgtg attcaaggaa tagaaaagta
    12421 taaaaataag tatatagctt atagtctagg gaatttttgc tttggaggaa ataagaatcc
    12481 ttctgataca gatagttata tatatcaaca aacctttact ttttcagata ataaattaac
    12541 ttcaattaaa gagccaaata taataccaac ttcaataaca tctagtaact ccagaaataa
    12601 ttatcaacca aaaatattgg atggaagtga gaaggataga gttttaaata aacttgaaaa
    12661 aataagcaaa gatttaaata aataaaatgt aaaaaaaaat aaatatatat atttatttta
    12721 ataatttata ttttgtttag ggtatggtaa atcgtggtat taaataagta gattttaata
    12781 gttaaattat agtcaattat aataaagcta tttaatctac ttttttaata taaaaatata
    12841 agggggaatt tgtaatggct tgtaacactt gtaaaatgtg cgaaagtgca gacaagaaat
    12901 tagaaagttt tgtagcttca aaggatgttg aaacagcatt tcatagaaca gaagatcaga
    12961 aagttaagtg tggttttgga cttcaggggg tttgttgtag actctgttca aatggaccat
    13021 gtagagtaac tcctaaatca ccaagaggta tatgtggagc agatgcagac actatagtag
    13081 caagaaactt tttaagagca gtagcatcag gagcagcttg ttatttacat gtggtagaaa
    13141 atacagctaa gaatttaaaa aatgttggaa taactaaagg tgttgttaaa ggagaaaaaa
    13201 cattaaatca attagcagag atgtttggaa tagaaagtga tgaaaagtat gataaatgta
    13261 taaaggttgc agacaaagtt atcaacgatt tgtataaatc aagagatgat aaaatggaat
    13321 tagtagaaaa aatagcttat gcacctagag tgaaaaagtg gaaagagtta ggtataatgc
    13381 ctggtggtgc aaagtcagaa gtttttgatg caatagtaaa atcatcaaca aatttaaaca
    13441 gtgacccagt agacatgcta gttaattgtt tgaacttagg tatatcaaca ggattatatg
    13501 gtcttacact aactaatctt ttaaatgatg ttatgttggg agaaccagtt ataagaatgg
    13561 ctcctgtagg atttaatgta atagacccag actacataaa tataatgata acaggtcatc
    13621 aacattcaac atttgcaaat ttccaagata gattaaaaga tgaagatgtt ataaaattag
    13681 ctcaatctgt tggcgcaaaa ggatttaagt tagtaggatg tacatgtgtt ggtcaagatt
    13741 tacaattaag aggagaacat tatcaagaag tatttgctgg acatgcaggt aacaatttta
    13801 caagtgaggc tgttttatca actggagcaa tagatatagt tctttcagaa tttaactgta
    13861 ctataccagg gcttgaacca atagcagata aatataaagt aaaaatggtt tgtttagatg
    13921 atgttgctaa aaaagcgaat gcagaatgca taggtttaga cagaagtaaa ttagatgaac
    13981 ttagtaacac tttaatagaa aaagcattgg agtcttataa ggagagaaga ggaagtattg
    14041 aaatagatat accaaaagac catggatttg aacaatcttt gactggtgta agtgaaaaga
    14101 acttgaaagc gtttttagga gattcatgga aacctcttat taatttaatt gcagaaggaa
    14161 aaattaaagg tgttgcagct gttgtaggat gttcaaatat gacagcagga ggacatgatg
    14221 taaatactgt tgaacttact aaagaattga ttaaaaaaga tataatagta ctatctgcag
    14281 gatgttctac tggaggatta gaaaatgttg gtcttatgtc acctggagca gaagaattgg
    14341 caggagaaaa cttaaaagaa gtatgtaaga ctttaggaat accaccagta ttaaactttg
    14401 gaccatgtct agctatagga agactagaaa tagtagcaac agaattagca gctgaacttg
    14461 gaattgacct accacaatta ccattagtgc tttcagctcc acaatggttg gaagaacaag
    14521 cattagctga tggagctttt ggtcttgccc ttggcttacc tcttcattta gctttacctc
    14581 catttataac aggaggaaaa ttggtaacag aagtattaac agaaaaatta aaagacttaa
    14641 caggaggaca tgttatagtg aatccagacc ctaaatcttc agctaatcaa ttagaagaaa
    14701 taataataga tagaagaaaa aatttagggt taaaggatgt ggaatgcaat gcatagaatt
    14761 tttataaata aagatttatg tacaggatgt aagagctgtg tactagcatg tatgctaaaa
    14821 cacaataaag attatgacat gtacactctt gatttagaaa atatagataa tgatagtaga
    14881 gggcatatag aattagatag tagacataat aatccagttc caattctttg tagacactgt
    14941 gatgaacctg aatgtgtatt agcatgtatg agtggtgcaa tgcataaaga tagtgaaagt
    15001 ggaatcgttt catatgatga agagaaatgt ggttcatgtt atatgtgtgt aatgtcttgt
    15061 ccatatggat tattaaagcc agatgataga agcaagcaga acatacttaa atgtgattta
    15121 tgtaaagatg aggagtatcc tagatgtgta gctaattgcc caagtggagc aattgaatta
    15181 cagaaggagg aaaatgatga attatgtagt gttaggggct agtgcagctg gtataaatgc
    15241 agtaaaaaca ttaagagaat tagataaaga ttcaaatata gtagtaattt ctaaagatga
    15301 aaacgtatat tcaagatgta tgttgcatca cgttatatct gaacacagaa ctttaaaaca
    15361 aataaatttt gttgatgaag attttatgga gcaaaataat gtaaaatgga ttgcaggtaa
    15421 gactgttaaa ggtatagaca taaataaaaa ggtagttcag acagaagaca ttactgtaaa
    15481 ttatgataaa cttttaatag ctacaggtgc atcttctgct attcctccta taaaaaattt
    15541 aagagatgga aattttgtat attctgtgag aaacatagat gatatttata aaataaaaga
    15601 aaaagcacaa aattctaaaa atgtagttat aataggtgct ggtttagttg gtatagatgc
    15661 attagtggga ttatttaaat atgagcagtt aaatatttct gtagctttta tggaaaagta
    15721 cattttagat agacaattgg atgaatatac agcatcagtg tatgaaaata agttcaaaga
    15781 aaaaggtgta aaattttacc caagtgcatc aatacaagaa attgtattgg ataacagtaa
    15841 aaatgttaca ggtgttgcat tttcaaatgg tgaagtttta gatgcagaca tggtaattgt
    15901 agctacaggg gttaaaccaa atgctgactt tttagatgga acaggaatag aatatgatag
    15961 aggtataata atagatgata tgtgccaaac aacacaaaaa gacatatatg cagcaggaga
    16021 tgttgtaggt aaaaatgcta tttggcctct ggcagtaaag caaggaattg tagctgccta
    16081 taatatggtt ggaaaagata agaagataga agatgaattt gcttttaaaa atagtatgaa
    16141 tttcatggat ataccaacta tttctatagg aatgaataca cctgtagatg atagctataa
    16201 agtattaacc agacatggaa ttaatgatta taaaaagttt gtttttaaag ataatgtaat
    16261 ctatggagca gttatacaag gagatatttc atatgtaggt gtattaactt atttaataaa
    16321 aaataaagtt gaaatatatg atttagaaaa taggatattt gatatatgtt atgcagactt
    16381 ttttaatata aaagaaaatg gagaattttg ttatagtgta tagtatatac gtggagtaag
    16441 gtttataaat acagtaaatc aataaataga gttattctaa aataagaaaa atgacaaatc
    16501 ttatttagaa atagctctat tttttgtaat taaatttaaa ttatattcaa tttgtaaaat
    16561 attattttga acaattatac atatactctc tagttagagg taaatcattt tttatacctt
    16621 tagtgaaaag tatttgatgt atatctataa taccagtgtt gaaagcagca gcacaagaac
    16681 aaaggtataa atcccacatt cttataaact tatcatcaaa catttcttta atctttggaa
    16741 gattctcaga gaaatttttt tgccagtata gtaaggtttt tacataatgt aatcttaaac
    16801 tttctacgtc tatagtatga tatttgtaat cactacaaat atgtatcatt tctcttaggc
    16861 taggaataac tcctcctggg aaaatatatt tttttatcca agggtctcca taagattcct
    16921 gcaatccact tatatagtga agtaaaaaca atccaccatc ttttaaaaca gcatttacgt
    16981 ttttaatgaa aagttcatag ttttctctac caacatgttc aagcatccca acgcttacaa
    17041 ctctatcaaa cataaggtct gattttttta attctctata gtccataagt cttacctcaa
    17101 gatagtcttg aaggttctct tctttaattc ttgcgttaaa ttccttaaat tgctcttcgc
    17161 taagtgtaat tccaacgccg tgaattttat atttttttgc agcttctatt agtaaaaatc
    17221 cccaaccaca accaatatcg agaaggctca taccttcctc taaatttagt ttagttaata
    17281 tgtggtgaac tttattgact tgagcatcat aaagagtatc attatcattt ctaaagtatg
    17341 cacaagaata actaagtgtc ttatctaacc ataagctata aaaatcgttg ccaatgtcat
    17401 agtgggcatg tatttctttc ttttgatgtc tcattgaatt tgatgagtgc aatagatgtt
    17461 ttacagctga attatcaata ttgaatttgt ctatttgttt taaaagtaga tttaaagtta
    17521 agaataaatc tccctcaact tcaatgtctc tatttatgta ggcttctcca agtgctaaag
    17581 atgtactttt taatagttca gacttgctaa tatcattatt tactataact ttgaactgag
    17641 gggagccctc tccaattaga aattcttcct catctttaag cctaactaga aatggtgttt
    17701 caccaaattt ctctaaatag tcttttaaaa aattttttgt aatattcata ttaccagctc
    17761 cttctaatgt aaatccagta agtttccctg cttaaaatat attatagtcc tttttttaaa
    17821 aaagtaaaat tttttcaata aattttttat ttaaaggaaa tttatttttt gtaaagaaat
    17881 ttcatgttgt agtatatttt ttgcttataa attttggtac tggatataat attaaactgt
    17941 taatgatata ataaatttaa tctataaagt aaataaaatt ttgtaataaa tagttaaagc
    18001 tattttataa ggagatatat gatggaagta tatgctttgg ttgggtcaag tggcacaggg
    18061 aagagcttta aggctttaga gttcgcatat gaaaatgata tagaatatat aattgatgat
    18121 gggattttaa tatacaaaaa caaggtttta gctgggatat cggcaaaaca agcaaatact
    18181 actatagaag cagttaaaag ggctattttt tataatctgg agcatagaca agaagttaga
    18241 gaagcaatta aaaaagaaaa tataagaaaa attcttatta ttgggacatc aaaaaaaatg
    18301 gttaatcaaa ttgtagagag attgtcaatt ggaagggttt gcaagtttat aaatatacaa
    18361 gatataagta ctaaatatga aatagaaata gcaaaacaag ctagaaaaga aggaaatcat
    18421 gtaattcctg ttccagcagt tgagataaaa agtatggcaa gtggtcttag tataaattct
    18481 ttgaagagac tttttagaaa aggaaataat aggaatatgg tagttttaga aaaaacaatt
    18541 ataagaccaa cttttagtta tataggaaag ttttatatta gtgctgatgt aattaaacag
    18601 attattgaat atgaagtata caactttgga agcattgata gaataaataa aatcaatata
    18661 gagaattgca ataattttat gaatattttt gtaagtataa atatcaatga tttagaaact
    18721 ataaaaaaaa ttgaagacat acaacagaca ataaaaaagt ctattgagaa aatgacttta
    18781 gttaatgtac aaaaaataga tattaacatt cataaaatta aaaaaataaa cttcctccaa
    18841 tgttaaaaaa taaactgaga aaatgatata ctaatttgca ataaagtgtg ctataattaa
    18901 actgtaaatt taggaaatgg ttttttctaa atggagtata cataattata acgtatttat
    18961 aagggggctt tcaaatgtca ggaaaagatg taaatgtctt cgagatggcg caatctcaag
    19021 taaaaaatgc atgtgataaa ttaggtatgg aaccagcagt ttatgaatta ttaaaagaac
    19081 ctatgagagt tatagaggtt tcaattccag taaaaatgga tgatggttct ataaaaactt
    19141 ttaaaggatt tagatcacaa cataatgatg cagtagggcc aacaaaaggt ggaataagat
    19201 tccatcaaaa tgtttcaaga gacgaagtaa aagctttatc tatatggatg actttcaagt
    19261 gttctgtaac aggtatacca tatggtggag gtaaaggtgg aataatagta gatccatcaa
    19321 ctttatctca aggtgaatta gaaagattaa gtagaggata catagatgga atatataaat
    19381 taataggtga gaaagttgac gttcctgcac cagacgtaaa tactaatggt caaataatgt
    19441 cttggatggt tgatgagtac aataaattaa ctggacaaag ttctataggt gttataactg
    19501 gtaagccagt tgaatttggt ggttctttag gaagaacagc tgctactgga tttggtgttg
    19561 ctgttactgc aagagaagct gctgctaaat taggaataga tatgaaaaaa gctaaaatag
    19621 ctgttcaagg tataggaaac gttggttctt atacagttct taactgtgaa aaacttggtg
    19681 gtactgttgt agctatggct gaatggtgta aatcagaagg ttcttatgct atatacaatg
    19741 aaaatggttt agatggtcaa gctatgttag attatatgaa agaacatggt aacttattaa
    19801 acttcccagg agctaagaga atatctttag aagagttctg ggcttcagat gttgatatag
    19861 taataccagc tgcattagaa aactctataa ctaaagaagt tgctgaatct ataaaagcta
    19921 aattagtttg tgaggctgct aatggaccaa ctactccaga ggctgatgaa gtatttgctg
    19981 aaagaggaat agttcttact ccagatatat taactaatgc tggtggagtt acagtttctt
    20041 acttcgagtg ggtacaaaac ttatatggat actactggtc agaagaagaa gtagagcaaa
    20101 aagaagaaat agctatggtt aaagcatttg aatctatttg gaaaatcaaa gaagagtaca
    20161 atgttacaat gagagaagct gcttacatgc attcaattaa gaaagttgct gaagctatga
    20221 aattaagagg atggtactaa gatttactct taataaataa taggatataa ttatttaaga
    20281 tataaagggg ctgtacatag tacagccctt aaattatgtg gcttacaatg aagtaagcta
    20341 tttttgtttt aacttataaa taacttgata aattatgtaa tttagaatgt attttttacc
    20401 accacatgat tacaaattag aggagttaga ttgaattttt agacatgaac ttatgcattt
    20461 taaaagcaaa gaccttaatt taaagtatct agtattattt ttaaaaatag tatattggtt
    20521 taatccattt atatgtataa tggaaaaata tatggatttg gattgtgaac tttattgtga
    20581 tgagagagtt ttaaaaaatt gtactgtaca acaaaagcaa gaatatgctc taactattgt
    20641 aaattcaatg aaaagaagta caaatttttc aaataaattt gtagctggat taaataaaca
    20701 atctgatatt agaaaaagag taataaacat gttcagtgaa aaacataaaa atggtatctt
    20761 aagggctttg attataagct tgttttcttc tattacattt tttaaattcg attcaactag
    20821 tattattgaa gagaatgatt tcttagagag gtcaagatat acgtttgaag gaaggcaaga
    20881 agacatgttt gtaacaatag attttatata tgcagatgta cctataaaac ttagaaaaga
    20941 gcatgaagaa aactgtagag tcaatggaat agtaccaaaa gattcagatt caatagaaat
    21001 tattcctaga ttttataaag attgtatgga tttaaaaaaa taagttagac ttaatattga
    21061 tgttgatatt atgaattttt tatatttaag caattgaaaa ttttattata gattcaattt
    21121 tatatgtgtt taagtatgaa atttatagat agttgaaata attaaatatg attgatgcat
    21181 aggaatcatt actaagctat aattaattat aggggagttt aaataaatta tgagttacac
    21241 aatcataaaa tgataaaaat agttatctgt tacaaaaaat gtatcttttt aattaataaa
    21301 gataatatga aaacttaaaa ggcaaataaa cttaaatata aaatactctt aaataagata
    21361 ggagaaaaat gtatgtctaa ctttattcta aatgggattt ttagaacagt tatagtaagt
    21421 agtctaagca ttcttctgat attgattttc aagaaaaatg tatttaaaag atttagcaaa
    21481 aaatttaatt attatatatg gatgatagtt gttattaaat tatttttacc atttacatat
    21541 tatactttta caataaatat cttgagaagt aaaaaacaca ttaatattaa taacataaat
    21601 ttagagggat ttaataatgt ttcaactata agtaataata ttatattgta cacttggatt
    21661 ataactgtag tagtgtattt aatttacact atatttaaat atataaaact taaaaattta
    21721 ataaatgact tatcttatga tgttgatgat agagagattg taaatcttta taaaaatata
    21781 ttagaagaat ttaatataac aaaagatata aaactcaaat actcttatga agttgaaaca
    21841 ccagcatttt ttaattcatg tgtacttttg ccaccacatg agtataaatt gaaagagtta
    21901 gactggattt ttagacatga gcttatgcat tttaagagta gggaccttta tttaaaatat
    21961 gtaatcctat ttttaaaaac agtatattgg tttaatccat ttatatatat aatggataaa
    22021 catatagatt tagattgtga actttattgt gatgaaagag ttttaaaaaa tcgaaattca
    22081 gaggaaaaaa aggattatgc tttaacaatt ctaaatgcaa tgagaaaagg ttcaaatact
    22141 tcaaataaat ttatagctgg acttcataag caatctgata ttaaaaagag agttatgaat
    22201 atgtttaatg aaaagtacaa gaatggtata ttaatggcat taactttatg tttattatct
    22261 tctattactt ttttgaaggt taattcaact agtattgtta atcttaatgt attacctaaa
    22321 ggatttgaag acttacgaac tgttagcagt atacgttttt cagttatagg atttacatat
    22381 gcagatgctc ctaaaaatat gagggaagaa tataaaataa attgtgaatc attaggtttg
    22441 atacctaaag actctgataa aatagaagtt ggtatagaat attatgacag tctattgaat
    22501 gattaaataa atattatttt aacagatatt aattataaag ttttgaatag taatttgttg
    22561 agaaataaat atatccttat aaaaatagat acttatatac caagaataaa aaatagtcaa
    22621 gatagtacac aaggatatac ttataaagaa cttaaataag tgaaataaat tattaattat
    22681 aaggtttata attttaaaaa attagataaa ttcaggtgta aaatactata aaacacaatt
    22741 cggaaaagtg tatgttgaac ttcatttaaa atggaatttc aaagttgcta tagtaagtag
    22801 tttaagcatt cttcttatat taatctttga gaataatgta tttaaaaaag ttagtaaaaa
    22861 atttaactat tgtatattaa tgatagttgc tattgatata ttatctaaaa aaattaagta
    22921 tgaaaagatt actgctagga caatcttttc atacttaatt tttaatattt atattatcca
    22981 aaattttcat ctttttaact tagaatttga gaatcactat taggaaatag gctttgattt
    23041 atgtagggtt aaaaatttca cagaatatct aactttatac taaatccatc aaaaaatata
    23101 cgccatacaa aagcgcataa aaaaacacat aaaacaatat ttatgcgcac tttaacctcg
    23161 aatggtacaa aattctttac ttttacagac ccatattata taattaagta aataatgtat
    23221 atttaggtga catattggat aagctaattt atttatttta ttaatataat aatactataa
    23281 aagttatata aagtggttta caaaaaagtt acagatagag cttttattaa tgtattgtgt
    23341 attagtattt tatccttagg tacacttcac ctacaaaata gaatcataag ataattatgg
    23401 gaaaatttta ggaggtttat cgtgaagaaa aagatattaa taccagtatt tgcatcagtt
    23461 atggcattat cagtaagttc aattgtaaat gcagatgaag taaatgattc atctcaaaat
    23521 aaggatgata aaacaaatac tgaattaaat ttaggtgaat ataaagaagt aaaatataaa
    23581 gttgcaaaaa taaaagatgg agtagctata aagataagag aagaaggtca agttcaaaac
    23641 atagcttatt ctggagatga atttacggtt ttaggaactc aaggagaatg ggttaaggtt
    23701 aaagttgaag atggcgaagg ttggttagct actagatatg tagatatatc tgaaggtgta
    23761 ggatatacta acgctgataa agtaaactta agaaaagata agagtgaaag ttcagaagta
    23821 attgaagagc tggaaaaagg aagttcttta ttggttttgg aagataatgg agattggtta
    23881 aaagttaaag atggggaaac agaaggatat gtaaaatcat cttatatatc tgataaagct
    23941 cctgtaatag aagagccaca agtaaaccca agtattgacc aaaatgtaga ttctacaaat
    24001 aataatcaac aaaacaatgc aaatactaat caacaaaata ataattcaaa tgtgccaaca
    24061 gctaactcta atgcagttca agctgtactt aacttggcat attctaagca aggttgtcca
    24121 tatgtttggg gagcagaagg accaaacact tttgactgtt ctggatttac acaatatgtg
    24181 tatagaaatg cagtaggaaa gaatatacca agaacatcta aagcacaatc taaatatgga
    24241 caaactgtaa gtaaagcaaa tttacaacca ggagatttag tattcttcac aactaatggt
    24301 tctggttctg ttagtcatgt tggaatatat gtaggtgggg gtaatatgat acactcacca
    24361 tcaacaggaa aaacagttag tgtaactagt ataaattcat catactatac agcaagattt
    24421 gttacagcaa aaagaatatt ataatcggtt tgtaatatga caaattgata aatacataaa
    24481 tatatataaa cgctctatat tatatttaat ttaatcatgg aaacaaaata atgattatat
    24541 tttaaatgta ctatagagcg tttttgtatc actttaaatc taatcaagat tcaagttaat
    24601 atgactgtta aataggagcc ttttcatatg atgattattt gtgcaatccc ttgacaggat
    24661 agggtaaagg tggtaagcta ctaacagtac aaaaatactt ttaattttag ttcagagagg
    24721 ctaaaaaagg aggacgaaag atgttaaaat caagaaattt aatccaacca gaagactttt
    24781 caatagaaga aatagatgaa atattagaat tagctcaaaa gataattgac aatccgtcaa
    24841 aatattctcg tatatgtgaa ggaaaactat tagctacatt attttatgaa ccatcaacaa
    24901 gaacaagatt aagctttgaa tctgctatga acagattagg aggacgtgta gtaggctttt
    24961 cagagccaaa ttcatcctct gcatcaaaag gtgaaactct aggagataca atgaggatag
    25021 tatctggtta tgttgatata atagctatga gacatcctca atcaggtgca gcttcagaag
    25081 cagctagata tacggaagtg ccatttataa atgcaggaga tggcaaaaac cagcatccaa
    25141 ctcaaacact tacagactta cttactataa aatcattaaa aggaacttta gaaagccata
    25201 caataggatt atgtggtgat ttaaaatatg gaagaactgt gcattctttg gttaaggcta
    25261 tggcaagata taaaaataca aagtttgtat ttatagcacc tgaagagtta aaaatgccag
    25321 attatataaa agaagctata aagggacatg catattatga aactaataat ttggatgatg
    25381 ttataggaag cttggatgtt ttatatatga ctagagttca acaagaaaga tttgaagata
    25441 aatcagagta tgaaagactt aaaaactatt atattttaaa taaagctaaa ttagaaaagg
    25501 cgtctaaaga tatgttagta atgcatccat taccaagagt aaatgagata gacatagatg
    25561 tagatagtga tgatagagct gtatatttta aacaagctaa atatggaatg tatgttagaa
    25621 tggctttaat aataaaactt ttaggtataa atgaagacta atagttatca tataatagta
    25681 aaacaaagga gattttggga tgtataaaat tctggaaaat atatatatag gcgaagatat
    25741 gtatagaatg aaagtaaaag gtaattttga aggtaaaatg ggacaattct atatgttaag
    25801 agcatgggat acttatccag tactttcaag acctataagt atacatgata tagatgaaga
    25861 agggataact tttctttata aagtagtagg agaaggaact caaattttga gtaacctaaa
    25921 ggtaaatgat actataaaat tggaaggccc ttatggtaat ggatatgcta aggttgatgg
    25981 aaaggtagct ctagtaggag gaggaatcgg tgtagctcca ctatatttgg ttgctaaaaa
    26041 tataaaaaat tgtgatgcat atcttggctt tagagaagat gttatattag aagatgaata
    26101 taaacaagta tgtaataaag tatatacaac agtaggaaat acttttgtaa cagatataat
    26161 tgatgttgag aaatatgatt atatattaac atgtggtcca actccaatga tggaaaaatt
    26221 agttaagatg gtagaaggta ctaaaacgag aattatggta tctcttgaaa accatatggc
    26281 ttgtggagta ggtgcctgtt tagtatgtac gtgtaaaact aatggaggaa ataaaaagac
    26341 ttgtaaagat ggtccagtgt tctggggaga ggacgtgatt tttaatgggt aatttaagtg
    26401 tgaaatttgg aagtgtagaa tttaaaaatc cagtaataat ggcgtctgga acttttggat
    26461 ttggaaaaga atataatgag atttatgata tacaaaaact tggtggtata agtagcaagg
    26521 gtcttacttt aaataaaaaa cctggtaaca atggtatgag agttcatgag acaccatccg
    26581 gtatgatgaa tagtgttggg cttgaaaacc ctggagttca aggatttata gactatgaat
    26641 tacctttttt tagtaaacta gacttagtca gaatagctaa tgttggaggt gggacattag
    26701 aagactactt attaggggtt caaatgttaa atgacaagcc tatagacatt attgaattaa
    26761 atatatcttg cccaaatgtc aaagctggtg gaatggcttt tggtataaaa aatgaagtgg
    26821 caagagaagt tgttcgtgag gtaagaaaca taacgaaact gcctttagta ataaaattat
    26881 cacccaatgc agaagatatt gtaggaatgg caaaagtttg tgaagaagaa ggtgctgatg
    26941 gagtttcatt agttaatact tttaaagcaa tggcaattga tataaaaaat agaagaccag
    27001 tatttgaaaa tgtatatgct gggttatctg gaccagctat aaaacctata gctttgagaa
    27061 tggtacatga agtgtgtaaa aatgtaaata tacctgtaat gggaatgggc ggaataacta
    27121 aagccacaga tgctatagag tttataatgg caggagctac ttgtatccaa gttggaactg
    27181 caaattttat gaatcctaga ataggtattg aaataataga tggaattaat gaatttatgg
    27241 atagagaagg aataaaaagt ttagacgaaa taagaggaat tatataaggt agaataaata
    27301 gtattttaaa gataaaaaat atagattaaa taaacaataa ttttataaca ttaacatgga
    27361 ggaaaaataa tatgagtaaa gtagatgtag tggatatatt aaagaaaagt gatgcattgt
    27421 tggagggaca ttttttatta tcttcaggaa aacacagtaa tagatacgta caatgtgcaa
    27481 aagtattaag atttccagaa tatgctgctg aggtattaag tacagttgtt gaacaaataa
    27541 aagacttaga tatagactta gtagtaggac cagctatggg tggagtaata gtttcttatg
    27601 agttaggaag acaattagga aaagaagctg tatttactga gagaaaagac aatacaatgg
    27661 agttaagaag aggatttgaa gttaaaaaag gagcaaagat aataattgct gaagacgttg
    27721 taactactgg taaatcaact atggagacaa aaagagtatt agaagcctta ggtggagaag
    27781 ttgtaggtgt tgcatgtata gcagatagaa ctaatcatga tataggtatg cctatatata
    27841 gtgctataaa acttgatatt caagtttatg aatctgatga gtgtccttta tgtaaggaag
    27901 gaaaattacc agttgttaag cctggaagta gggagttcaa ggaattaggg atgtaataaa
    27961 aacttaatta tttatagtgt tacttaaaaa atgtaaattt tttagagtaa aaatttgtaa
    28021 tataacatat aacttaaaaa taaaaagcag ttagccttat aaatgaaggt tagctgtttt
    28081 ttttgttaaa aatatattta caaaataaga gatagtagca gtacaatata agtataaggt
    28141 aaaagtatta tatattaggg aggggtaagt atgaaaaatc aattaaatga aaatcataaa
    28201 aaggttgtat tttttggagt aggtgctgta ggagctactt ttgcagaaca attttttaac
    28261 tctaaatatg attttaaaat tctttgtgat aatgaaagaa agaaaagata tttagaagaa
    28321 ggatttataa taaatggaaa aaggtatgat tttgattatg taactaaaga tgagtataaa
    28381 caagaggctg attttataat tataggtcta aaatataata atttaaaaga aaatataaaa
    28441 gaattagatg gattagttgg aaaaaataca gttataatgt ctctgctaaa tggagttgat
    28501 agcgaagaga taataggaga aagatttgga attgaaaaaa tggtatattc atatgttacc
    28561 aatatagatg caaagaaaat caataataat attatacaca ctactaatgg gataattgta
    28621 tttggtaaca aagataatag tgaagataga aaaactaata taataaccga agtctttgat
    28681 gatgtaaata tagaatatac tttatcaaaa gatattcaac gagatatgtg gtggaagtac
    28741 atggttaata ttggtgtaaa tcaaacttca gctatacttg gtgcacctta tggagttttt
    28801 cagagttctg agcatttaag agaattagca aaatctgcaa tgagggaagt tgttgctata
    28861 gcacaagcaa aagacatatc tcttacagaa gatgatgtgg aacattcatt acatagaata
    28921 ctagaacatt caaaagaagg aagaacatca atgcttcaag atgtggaagc tcatagactt
    28981 acagaagtag atatgttttc taagaatatc tgcaaacttg gaaaaaaata taacatacct
    29041 actcctataa atcagacttt cttttatatg ataaaagtaa ttgaaagtag attttaaagg
    29101 gtctttgtaa gaaatattga taattattga ttttaaattt tattaatgtt atatactatt
    29161 taagttacta aatattttta actcttcaat gaaaatagtt gctgcttttg ataaagttat
    29221 atcttttgga aatatatatg agaaatgtct gttgtagctg cttccaagct ctatgacaga
    29281 cagctcattg tttagaacag gtaagcttgt cactagatta gagactattg taattcctaa
    29341 attatttcta acagattctt tgacagcata attacttcca aaaaccatca tactttttgg
    29401 aataatttct ttaacactta aaaacatatc taaataatct ctggttccag aaccatcctc
    29461 cctgactatc catttttggt tttgaagttt atcaaaacta aaatctttta ataaatgact
    29521 cttgtatgga agtgctaaaa ccatcttatc ttcaaaaaaa tattcttgaa taaatgaagg
    29581 ggaagagcaa gtgccttcaa taagaccaat gtctaaaata taatccttaa catgagaaga
    29641 tacgattgat gtatttttta taaaaacttc tacatctatg tcaggatact ttttagaaaa
    29701 taatgctaga aaatttggta aaatatactc tccaatagtt aaacttgcac caatctttaa
    29761 gtgaccagtt atagcatctg aaacttggct tacatcatga taagtaatat ttaaaagatt
    29821 taatatttct ttagctcttt tgtacaagat ttgtccactt tctgttataa aaatggtttt
    29881 ttgtttaata gacctgttta taagcacaac tttaaaataa ttttctagat tttttatgtg
    29941 tttacttaca cttggttgtg atagatttaa atattcccca gctttagtaa agtttttgta
    30001 ttcaacaaca gctacaaatg tttttaattc ttcaaacaat tttttgtctc ctttacctaa
    30061 tgaaatttta cattttttat atactttcta cagataatta tactatatat aataaccatt
    30121 aaaaacatta atcattacga tattatatat ttattttact tatggttatt acaattatat
    30181 actattgcta tgaagaaaac tatattaatt ataaggtagg tatattatgt taaagaaaaa
    30241 aagcagtttg acattaaaaa ctataaggga aatattacca ggactgtttg tatcagtatt
    30301 agtaggttat attagtatat ttatttctat tttaattcct aaggtagggg cagcatcaat
    30361 ttctatattt ttaggtatgt ttgtaggaaa cctattttta aatcaaaagg tatttcaaaa
    30421 aggttataaa ttttcagaga cagatttgtt atcatattca atagtactat taggagcaac
    30481 actcagtgta tctaccttaa tagacttaaa agtttcagga attttattta taatattgca
    30541 aatgacaata acaataatag ctgcacttta tataggtaaa aaactaggat ttgaagaaaa
    30601 ctttagattt ctaatggcaa gtggaaatgc agtatgtggt tcttctgcaa taggagcaac
    30661 agttccagtg gttaatgcga cagataaaga aaagggaatt gcagtaacta ttgtaaatgt
    30721 tactggtata tttcttatgt ttttacttcc aataatatct caatttttat atagccatga
    30781 acttgtaaaa acttcagcaa tgataggtgg aactttgcaa tcaattggtc aggttgtagc
    30841 aagtggagca attgttggag aaaatgtaaa agatttagct actattttta agatagtaag
    30901 agtaatattt ctagttgttg ttgtattagt atttggacat atgaaaaatc aatctaatag
    30961 ggaaattctt gaagaagaaa aggatgagat aaaaaagaaa aaagtgacta taccatggta
    31021 tgtaattgga ttctttataa cttgtgcact tttttcaatg aacattatac cacaagaagt
    31081 atctgtgttg tgcaaagaaa taagtaacaa actagaaata atagctttag cagcaattgg
    31141 attaaaggtt aatgtaaaag atttagtgaa acaagggaaa gaagtttctt tatatggttt
    31201 atttgtaggt atagtacaag ttgtatcagc tgtagtttta ataaaaatat ttatatagca
    31261 taaaaatagt atttgtaata ttaatttgat taattaaaat aatatgatac taataattta
    31321 taatataaat aaaaaatgaa tttcgataag tttaagtggg tataaactta tagaaattca
    31381 ttttttattt aaggtaaaaa tttaaagtaa aatattattt tacataataa taaccttata
    31441 aattttgaaa ttacttatct ttgtgttaaa ataagtaaaa tgcacatatt agagctaagt
    31501 ttttaagtaa ataaacaaaa tgaaaatata aatattaagg agatttggta tatgaatgtt
    31561 tatgaaaaag gtaatggagt tatactagaa ggagtaacag attttgaccc agtacatata
    31621 tttgagtgtg gtcaatgttt tagatggcac aaacaagaag atggctcata cactggagtt
    31681 gcaaagggga gaatattaaa tgtcaaaaaa gagaatgata aaatatattt aaataatact
    31741 aatttaaagg agtttaacag catttggtat aattactttg atttaggaac tgattataca
    31801 gaaataaaga ataagctaaa aaatatggat gaatatttaa ataaggctac tgaatttgga
    31861 tggggaataa gaatattaag acaagatgga tgggaaatgc ttatatcatt tataatttcc
    31921 tctaataata ggattcctat gatacaaaga gctatagaaa atttatcaag aaaatttggt
    31981 aagtatatag gtgaatatga aggtaatgaa tactatgcat tcccaacacc agaagaacta
    32041 aataaggcct cacaagagga aataagagca tgtcaaacag gttttagaga taagtacata
    32101 aaaagtacta cacaggcagt tatagagaat aatgatgagg tatctgaata tacaaattta
    32161 agtactgagg attgtagaaa agagctttta aaatttaatg gagtaggacc aaaagtatgt
    32221 gattgtattg cattatttgg tatgcaaaaa tatgattctt ttccagttga tgtttgggtt
    32281 aagagagtta tgcaagaatt ttatatagat gaagacatga gtttacctaa aatgagaact
    32341 tatggaatcg ataaatttaa agaaatgtct ggatttgctc aacagtactt attttactat
    32401 gcaagagaac tcggtatagg aaaataaatt aagggggaga aattatggga gtaataggaa
    32461 caatatttct gttctattta attatatcat atctagctgg agccataatt tctgtgatta
    32521 tattgcttga aaatagagac cctgcaaaaa caatgtcttg gctattgatg tttattatat
    32581 ttccaggtgt aggtttaatg atatatgcta tttcaggtag aaatattaga aaaagaaaat
    32641 tatttaagac tcaaaaacta gctaataata taaaagaaaa gaaactattt gatacattag
    32701 aaaagataac agaaatagtt gaattagaaa aagaatctat aaaacaaaat aagcttttaa
    32761 gagatgaaga ggatggtagt tatagaaaaa gggtaataaa tatgttgtta aagactggta
    32821 tgtttccctt tactaaaaat aataaagtgg atgtatttgt agatggaaat gaaaagttta
    32881 aaagacttat agaagatata agagaagcca aagaccatat tcatttagag tactttatca
    32941 tcaaagatag tgaaataggt agggtgttaa aagaagaact tataaaaaag gctaaagaag
    33001 gcattaagat aagaatttta tatgatgatg taggatgctg gagattttgg tttaatagaa
    33061 aatttttccg agaaatgagg gaagtaggta tagaaattgc agcattttta cctacaaaat
    33121 ttcctataat aggagggaaa ttaaactata gaaatcacag aaaaattgtt gttattgatg
    33181 gaataattgg atatacaggt ggaataaata taggagatga atatttaggc aaaaatgata
    33241 agtttggata ttggagagat actcatatta ggattaaggg tatttctgta tacatgcttc
    33301 aaatgacatt tctgattgat tggtattata cgacaaaaga agtattagtg actaaaaatt
    33361 atttcccaag tgtagggaat gttggtgaaa gtatgataca agttgttgcg agtggtccag
    33421 acagtgactg ggaagatatt cattatgctt atttttctgc aatatgtcag gctagaaaaa
    33481 atgtttatat agaaactcca tattttattc cagatgaaag tctactaaaa gctataaaga
    33541 gtgctgctct tagtggagtt gatgtgagaa taatatttcc taaaattgca gaccacaaga
    33601 tagtaaatat agcatcttat tcatattttg aagaaatatt aagagcaggt ggtaaagttt
    33661 atttatataa taaaggattt atacattcta aagtcgtaat aatagatgat aaaattgctt
    33721 ctgcaggtac agctaatatg gatttaagaa gttttatgct taactttgag gtaaatgcat
    33781 ttatctatga tgaagaagtt attagggtaa tgacagatga tttctttgag gatttaagtc
    33841 actgtgaaga acttaatttg gaggtattta agaatagaaa tatcatacaa aaaatcaaag
    33901 aatctgtagc aagattattt tcaccaatat tataaaaaat tattaaaaaa ggtattgaaa
    33961 attatgtgtg attggtatat tataataatt gttagcactc tattagatag agtgataata
    34021 aataaaaagt atatatataa atctacatat ataagtagta ttaatttaaa aataactaaa
    34081 aacataggcg aactagtcta tacttaatat tttaggaggc gtgaataatg aaaataagac
    34141 cattagctga cagagtagta attaaaaaag tagaagcaga agaaaaaact gcaagtggaa
    34201 tagttttacc aggagcagct aaagagcaac ctcaaatagc tgaagttgta gaagttggac
    34261 caggtggaat agttgaaggg aaagaaataa aaatggaatt gacagtggga gataaggtta
    34321 tattccaaaa atattctgga acagaagtta agatagaagg acaagaatac acaatactaa
    34381 gacagagtga tgtattagct gttattgaat aaatatagaa taaatttatt aggaggggtt
    34441 taaaatggct aaagaaatta aattttcaga ggaaacaaga agagctttag aagctggtgt
    34501 aaataaatta gcagatacag taaaagtaac attaggacct aaaggaagaa atgttatatt
    34561 agataaaaaa tttggttctc cacttataac taatgatgga gtaactatag caaaagagat
    34621 agaactagaa gatagatttg aaaatatggg tgctcaatta gttaaagaag tagcaactaa
    34681 aactaatgat gtagctggag atggtactac aactgctaca gttttagcac aagctataat
    34741 aagagaaggt ttaaagaatg taacagcagg ggctaaccca atacttttaa gaaaaggaat
    34801 acaaaaagca gtaacagtag cagtagaaga attaaaaaat caatcaagaa tagtagaaac
    34861 acaagaggct atatctcaag ttgcttctat atctgctggt gatgaagaag ttggaaaatt
    34921 aatagcagaa gctatggaaa tagtaggtaa agatggagtt ataactgttg aagagtctca
    34981 aactatgaat actgaattag atgctgttga aggtatgcag tttgatagag gatttgtttc
    35041 tgcatatatg gttacagatg tagataaaat ggaagcagtt ttaaatgacc catatatatt
    35101 aattactgat aaaaaaatat ctaacataca agaattatta ccagttcttg aacaaatagt
    35161 tcaacaaggt aaaaagttat tgataatagc tgaagatgta gaaggtgaag cattatctac
    35221 attagtagta aataaattaa gaggaacatt tgatgtagta gcagttaaag ctccaggctt
    35281 tggagataga agaaaagaaa tgcttcaaga tatagcaata cttacaggtg ctcaagtaat
    35341 atcagaagaa ttaggttatg atttaaaaga agctgattta tctatgctag gtagagcttc
    35401 atctgttaag gtaactaaag aaagtactac aatagtagat ggttctggag ataaaaaagc
    35461 tatagaagat agagtaactc aaattaaaca tcaagtagag caaactactt cagattttga
    35521 tagagagaaa ttaatggaaa gattagctaa acttgctgga ggagtagctg ttgtaaaagt
    35581 tggagctgct acagaagtag agttaaaaga aagaaagtta agaatagaag atgctcttaa
    35641 tgcaactaga gcagctgtag aagaaggaat agtagctggt ggaggaactg cttttgttag
    35701 tgttatacca gcaataggaa cactaattga gagtttagaa ggagaagtta aactaggtgc
    35761 tcaaatagtt aaaaaagcgt tagaagagcc attaagacaa atagctataa atgctggtct
    35821 tgaaggtgct gtaattgtgc aaaatgttgt taattctgaa gcagaaactg gatttgacgc
    35881 attgaatgaa aaatatgtaa atatgataga agctggtata gttgacccaa ctaaagttag
    35941 tagaagtgct ttacaaaatg ctgcatcaat agcaagtact ttcttaacta ctgaagctgc
    36001 tgttgctgac cttcctgaaa aagaagatgt aggaatgcca ggaatgggtg gcggaatgcc
    36061 tggtatgatg taatttaaaa aatttaaaat ataaatttta atataaagaa ctatttcgac
    36121 ttagatttta aactctaagt tgaaatagtt cttttgtata tatctaattt ttgtatatct
    36181 ataaattcaa tacacatatt tagataatca gtatctagtt tgtaataata ttttagagta
    36241 gtattgttat tttttaggtt atgattttag atatatatta attaaggtat aattgttgat
    36301 agagaataaa tcaaatttaa aaggatgcga taatatggac ttaagaaaat tgcttgaaga
    36361 agttaaaaat gaaaatattg atatagattt tgcgttagaa aagttaaaag atttacctta
    36421 tgaagacctt ggatatgcaa atatagacca tcatagagaa ttaagaaatg ggtatccgga
    36481 agttatttat tgtgaaggta agagtgatga gcatatttta gggattattg ataaaatgaa
    36541 tcaaaaaagt tctaacatat tggggacaag gtgcagaaaa gaaacttttc tgaaaattaa
    36601 agaaatctat aatcatgctg agtacgaaga ggcgtctaaa attcttaaga ttcaaaatca
    36661 tgatatagag aatataggaa aaggtaaaat tgtgattgct acagggggaa cttctgatat
    36721 accagtagca gacgaagctt atcatacagc taagttttta ggtaatgatg ttgatagaat
    36781 atatgatgtt ggagtggcag gtatacatag gcttttaaat aaaagacata aaatagatag
    36841 tgctagagta atagtagccg ttgcaggtat ggaaggtgct cttgcaagtg taattggtgg
    36901 actggttgat gttccagtaa tagctgttcc aacttctgtt ggatatggag caaattttgg
    36961 aggattagca gcattactag ctatgttaaa tagctgtgca tctggaatat ctgttgtaaa
    37021 tattgataat ggatttggag caggatattt agcatcaatg ataaataaac tgtaaataca
    37081 ttgatataaa ttaaacaaaa ctatataata aaatttaatt tctattatat ttttactatt
    37141 aaaattgagg agacatatat gagtgaaaaa atactttatt ttgatataat aagcggtata
    37201 tcaggggata tgacccttgc aagtttatta aatttaggtg tgcctaagga aatttttttg
    37261 gaggaaattc ataaactaag aatgagcgat gaatttaata taaacatcag ctcgaaaact
    37321 gaaaatggaa tagtaggtac aaaagtagaa gtaattacaa aagaaaaaca tacacataga
    37381 aatttagtgg atatatttga ggttatagat aaaagcactc taaacgaaaa tgtaaaatct
    37441 aaagcaaaaa aaatatttat ggtaattgga gaagcagaag caaaagttca tggaactaca
    37501 atagacaaga tacattttca tgaggtaggg gctatagatt caattgtaga tatagtagga
    37561 gcttctatat tagttgactt actttgtgta gataaagttt gtgctagtac agtgtctgtt
    37621 ggttcaggat ttgtaaaatg tgaacatgga attataccta tacctgcacc tgcaactata
    37681 gaaattttaa aaggtgtgcc aattaaatta aataatgtaa atggagaatg tacaactcca
    37741 acaggagctg caatcataaa agtcttgtgt gatgagtttg tagatgagct tgaattttct
    37801 ccaaagcaaa ttggatatgg tataggtcat aagaagtttg aagtaccaaa tatactcaga
    37861 actgtattag gtgaaaaaaa aaagaagaat taatttatga aattagtgca aacattgatg
    37921 atatgtcttc agaaatctat tcttatttgt atgaaaaaat attagatgag aaagcattag
    37981 atatttatac agagagcatt tatatgaaga agaatagacc agcaacaaaa gtatcaattt
    38041 tatgtaatga atcagattta gaaaaattta ttaaaatatt acttttagaa actagtactt
    38101 ttggagtgag atataatgca tataatagag aaattctaga gagaaaattt gtaaaattag
    38161 atacaaaata tggagtggtt acagtaaaac tagggtatta taaaggaaaa ctagtcaagg
    38221 ctactccaga atataatgaa tgtaaaacta tagcaaaaaa tgaagatatt cctataaaaa
    38281 aagtgtttgc tgatataaat tttagaataa aagaagaatt tagtgaaaac ttattgacat
    38341 aagtaataaa ttttgataaa ttaatattca gccttattaa ataaaagtaa acagaaaaaa
    38401 atatataaaa tataaactca ctcgtatatg ctcagaaata tggtctgaga gtctctacca
    38461 agataccgta aatatcttga ctatgagtga aattattata ccaaaagacc tagttaagta
    38521 aatgtgtagg ctgagttaaa ctccctatgt gttggctata agataaaggt attttggaag
    38581 cctgtatagt aatttcactt ttgctagaaa ttatggaagt tactatacag gttttttgtg
    38641 taaaaagatt taatataatt tagactgtat tgtgttaaaa taaatatata aaaactatat
    38701 tatacaagga gagatgtaaa tttatgaaac atgaattagt acttgtaata gattttggag
    38761 gacaatataa tcagttgata gctagaagag ttagagaaaa caacgtatat tgtgaaatac
    38821 ttccatgtac tgcaagcata gaaagaataa aagaaaaaaa tcctaaaggg ataatattta
    38881 caggcggtcc aaatagtgcg tatttagaag attctccaac aatatcaaaa gaaatatttg
    38941 aactaggagt accaatactt ggaatatgct atggtattca aataatgtct catgtattgg
    39001 gtggagttgt aagaaaaggt aataaacaag aaaaagaata tggaaaaaca gctataactt
    39061 atggaaaatc atcattattt gaaggaataa ctacaaatag tgtatggatg agtcatacag
    39121 atttaataga aaaagttcca gaaggattta ctatagttgc aaatactaat gattgtccag
    39181 tagcagctat ggaaaatgta gaacgaaacc tatatggagt tcaattccat ccagaggtag
    39241 aacactgttt ggaaggggac aagatactta caaacttctt atataatatt tgtaaagtaa
    39301 aaggtgactg gactacagat tcattcatag aagataaaat aaaagaatta aaagaaaaaa
    39361 taggcgataa aaaagcttta tgtgctttaa gtggaggagt tgattcttct gttgctgcag
    39421 ttcttattca taaagcaatt ggggacaact taacttgtat atttgttgac catggtttac
    39481 ttagaaaaaa tgaaggaaat gatgtagaaa gaatatttag agagaaattt gatataaatt
    39541 taataagagt taatgcagaa gatagattct tatctaaatt aaaaggagtt tctgaaccag
    39601 aagctaagag aaaaataata ggtgaagaat ttataagagt atttgaagaa gaatcaaata
    39661 aattaggaaa aatggatttc ttagtacaag gaacaatcta tccagatgtt atagaaagtg
    39721 gacatggaaa tgctgctact ataaagtctc atcataatgt tggtggaatc ccagaagatg
    39781 tagacttcca agaaattgta gaaccattaa gagagttatt taaggatgaa gttagaaaaa
    39841 taggattaga gcttggtata gaagaaggat taatatttag acatcctttc ccaggaccag
    39901 gtctaggaat aagagttata ggtgatgtaa ctaaagaaaa atgtgatata ttaagagaag
    39961 ctgatgctgt ttatatggac gagcttagaa aagcaggttt atacagagaa atatggcaag
    40021 cttttgcaac attaccagat gttaagactg ttggagttat gggtgatgaa agaacatatg
    40081 cttatttagt tggtttaaga gcagttactt cttctgatgg aatgacttct gactggtata
    40141 agatgcctta tgatgtatta gaaagaattt ctaatagaat aataaatgaa gttgatggag
    40201 taaatagagt agtttatgat ataacttcta aacctcctgg aactattgaa tgggaatagt
    40261 aaataaaaaa gtcgtaagcc ttgtattttc aaggtttgcg acttttaaaa aaatctttgt
    40321 gatactaaaa tgatactata tatttaaatt atctagtttt tgggcaactt caagttgctt
    40381 atttgggtat aaatgtgaat atgtattcca agttgtttct actttttcat gccccaatct
    40441 ttctgctata gttaatatat ttacatccat atttaccaat aatgacgcat gagaatgtct
    40501 caaatcatgc actctaattc tttttacatt agataactta cagcatctat ctagctcttt
    40561 agaaagataa cttttagcaa atttaaatat tctttcatta tcttttaagt catatagctt
    40621 agatagataa tcttttatat tattatataa gaaatgtggg atagggacaa cacgtttact
    40681 tttaggagtt ttaggagaag aaacaatatc ctctccattt agtcttatgt agcttttttc
    40741 gacgcttatt ttattttcga aaatatcttt tggagtaaga gctaacaact cccctagtct
    40801 aagacctgtc caaaataaaa tttcaaatgc tagtctaggt tctgatttct tttcaaattc
    40861 aataaatttt ttaaactctt ctaatgtcca aaagttcatt tcatctgcgt tttttttacc
    40921 gatagaacct gccaaatgag caggattaga aggcaagtta taatatttaa ctgcataatt
    40981 taatacagca acaagctggt tgtttatcgt tttaatatat gtttggctat aatcactttt
    41041 taataattcg ttttgccatc ttcttatatg tgttgcagta atttcattta tttttagact
    41101 tttaaaaaaa ggtaaaattc taaagtttat taaatacttt ttagtttcta atgtagataa
    41161 ttttaatcta gaagacatat cgtgcatgta ttcttctatg agactttcaa aactcatatc
    41221 agtactcatt ttagatttat tcaaaaattc tctttcaaac tctagagctt cttttttagt
    41281 tttaaaacct ctcttaattt tctttttcct atccccatcg aagtctgtaa aataaaagct
    41341 agcataccaa ctttttcttt gttcgtcttt gtaagcaggc attttagaca cccccaatta
    41401 tgtcatttta atattacaat aattataaca tgttagatat gctatctaaa taattgtttt
    41461 attttcctct tctttttatt tcttgaagct tgtaatattt acacagttct aagaagtctt
    41521 cttctagtgc tatagataag tctaatatcg tagatatacc tatatctttg tactcttgat
    41581 tttctagttt tgatatataa cttctgttac gtctcatttt ttctgctaat tctagttgtg
    41641 tcattctttt cttttttctt aattttttca acatttatta aaatccacct tttaattatt
    41701 tttatgacat ttttataatt aatagtttgt gtaatttctt gtaaaaaatg ttcctcatta
    41761 gaacattttt tggtggaaaa atgtgttaaa atgtaagtaa gaaaagcact ttcaaagcac
    41821 tataaaaata gtgctgaaat agataaacaa ataaaagtaa taaggaggaa agatagattc
    41881 tagagggaaa tcattatcat ttttaagaac gtatgttcag ttggtgggat agaaaaatac
    41941 tttacagggg atggtttatt tgaatgagaa tgaaaatatg agtttatatg tcagtaagtt
    42001 aaaagaagta ttgaaaaata attcagaaga gtataaaaaa ataagagcta aaataaatga
    42061 aatttataat ctaaatgaaa agaaagaata aaagggcttt agctctttta ttctttctta
    42121 gtattaactg ctttataata agcattatct atcatttttt ttattgcatt tttatcatct
    42181 gaatctaaag atgctaatct atttattagt tcgttaactt cattatcttc ttttgagtta
    42241 acttttttta ataattcatc atcttgttga gtgttaggtt gtattttacc taatttagtt
    42301 aatagctcct ttaaagttaa atttaatcca aaactcattt tttcaagcat atctaaagta
    42361 ggttcaacag ctttaccatt ccttgggtct ctgttttttt ctatcttatc tatatatgta
    42421 tggctcacac cacaaagatt agaaaattct ctaagagata agttatgttc acttctatat
    42481 tctttaataa tttctgctaa tgtttgcata atttcacacc tcacctaaat aatagttgac
    42541 ttaattatat cagaaaaatg taaactattg ttaacaaaaa taaaaattta gtaaaaaata
    42601 attgacaaga gtaaaaaaaa gacgtatact atgattaaca aaaaggttat aaataaagtt
    42661 aggagggatt aatataaaaa ataatttaca atatattaga aaagaatcta gaatatcaca
    42721 aaaaaaattc gctgataaga taggtatatc aagaccgtat ttatctagaa tagaaaacgg
    42781 taaggttaat ccgagtttag aaatagcgca taaaatctcg ctcgaaacag ggaaaacaat
    42841 aaatgaaatt ttttttgatt ttactgtaaa tcatagtaaa ctataaggtt ttaattgtac
    42901 cttgaaaact aaatataaaa tattttaaag gagcaagtat atgaaaaata aagaaaaaga
    42961 aataatcatt agaggaattt taataggaat tttttatttt ttaggaattg tatttagtaa
    43021 tacttttttt aaataataaa tagaaggggt aggtgaaata ccaactcccc aaatcaaaat
    43081 aacactttgg aagttaaata tagaatattc aaaagaggtg attagatgga aatagagcaa
    43141 acaacaatac gcctgctaaa aagcagacga aagttgaata ttaatttcta ttttggttag
    43201 gcgaaagacc taacaaaaaa tctgtagata cattaagttt gttagaaatt aatattaaag
    43261 tttctatatt tggctctctt gaacccgatt cataatattg atatgcacgt tctgagatac
    43321 caaacaaact agcaaattgc ttttgagtca tattcatttg ctttcttact agcttaatat
    43381 tatctttgaa tttagtcata actacacgct ccaataaaaa aataaataaa tttcttgaca
    43441 cgaacaatat tagcgtgtaa tatataaaac atagcacgaa caatattggc gtgttaaata
    43501 aaaaggagag ggtgaaaata aataacaatt taaaactaca acgtgaaaaa gttggcttaa
    43561 cgcaattaga ggttgctcaa aaagccaaaa taacagagag aagctatcaa tattatgaag
    43621 ctggcgaacg tctcccaaac attcgtacag ctttaaagat agctagaatt ttaaatacta
    43681 attgtgaaaa actctttaat gaataatagc ataaaaggac tcagattaca agaaaggagc
    43741 tttagatatg aataatttgc aacttaacaa taaaaataca ataacaacat tagaaattgc
    43801 tgatatgtta gaaattaacc attgggaagt attgagaaaa ttagaaggca cagaaaaaac
    43861 aaaaggaatt attgatattc ttaacgacaa caattttgtt gtggttgatt actttataaa
    43921 atcagcttat ttagatttaa aaaatgaaaa tagaaaatgt tacaatgtaa cgaaattagg
    43981 ctgtgacttt ttagcaaata aatttactgg agaaaaagga attattttta cagctagata
    44041 tgtaaaaaga tttaataaaa tggagcaaga gttaaaagaa caacaaccta aactaccaac
    44101 tacatacaaa gaagcattac aacagttatt aatagaagtt gaagaaaaag aacaattaca
    44161 attagaaaat caagaaaaag ataaggtaat tcagttacag caaccaaaag tattatttgc
    44221 tgattcggta gcgtcttctg acaattcaat cctagttgga gaattagcaa agttgcttag
    44281 acagaatgga attgatacag gacaaaatag attatttgac tggttaagaa ataatggtta
    44341 cttaataaaa cgtaaaggtg aggattacaa tacaccaact caaaaaagtg tagatttagg
    44401 agttatagaa acaaaagaag gtacaagagt acatccagat ggtcatacaa gtattactaa
    44461 aacacctaag attactggta agggacaaat atactttatt aataagttta aaaagaacaa
    44521 tcaaatatca atgttaggtt aaagattaat agcactttga aaactaaata cagaatattt
    44581 tgaaatatat tgttttaatt aattattaac taggaggtta aatacatgaa aaataataca
    44641 agcgattcaa gagtaaaata tttttgcaag tgtccatatt gtgggtttga taatgaggta
    44701 gaagttaaaa aagggtcgaa gcctaaaata tgttgtatat gtacaaaaga agttgagtat
    44761 gaaaaattgg agcaacaaag tgttttggaa aataccgaaa ttaaagaagt atgtaatcaa
    44821 atgaaaatta aaaaattagc actagcaaca ggagaagtat ttgaaaatat agagctcaaa
    44881 gaatttgaaa ataaaggaga atgcttacca ccaaatatgg taaaggttat tgatggtaag
    44941 caagaacttc taataaataa agagttcata ttatcattag aagtcaaaag aaagactact
    45001 ttttaggacc tttcttagtt tgtgacaaag cactaccagc aaccgatttg gaagcagcac
    45061 tatatcttcc gtctttaaga atcttactag ccttagaagc aacagtttta cttgtttgtt
    45121 tagtattttt agctataggt atcaccacct ttaaatgtat ttataggatt tatcctacaa
    45181 atatagtata tcaaaggagg gaaataatgg caattaatga caacataaat aaaattttaa
    45241 gagatagaga tttaaaagcg tggaaattag caaaagaaat aggcgtagat tcaggaaatt
    45301 tatatgcaat tttaagagga gaaaataaaa atccaactat agatacattg ataaaactag
    45361 ctgactattt agatgttaca ttagacgaat tagttggaag atagaaatta aatacagaat
    45421 attttgaaag taggtgtatt gtatggcaaa agcagtagct aaagaacaat tgttttatag
    45481 ggcaaaagat atagctaaga ttttagatgt atgtgaagca accgcttata aaataattgg
    45541 agaattaaat aaagaattag aaaaagaagg ttggaagacc tttcctggaa gggtatcagt
    45601 aacttatttc aaagaaagat attgttataa aacaaaaaaa ggggcataaa aaatgctatg
    45661 agagtagcta aagatatgtt tcctaaagta attgtataaa gaggaagggg gtgatttagt
    45721 tgaatgtaag agtactgata gcttatatac agttttgtaa gcaatacaat aagaaagcga
    45781 gttttgaagg tcttaaaaaa tacaataagg ggatagtgat ataaaaaaac ttaaagtaaa
    45841 atttcctaca aaaattctta aagcaggtaa agcaatcaga gtaacatgtg caagatttgg
    45901 ttttgaaagc gattgtataa taactcaatc gcaagaattt gaattaacag tagtttattt
    45961 tgataaagaa ttagatgatt tagtacaatc gtctataact atagatgatg caataagcta
    46021 tgattattgc attgaaacgt taaattaaga ggggattaat catgaaaagt ttaattatag
    46081 tgagaaattc agtagagcaa caactaaata gagctaattt agaaataaat aaaaacgagc
    46141 aactttatac aaaacttaga aaaaaagaag aaagaaatat atcagaagaa attgaattga
    46201 gtaatgcttt aagagaaaaa agtgtaaacg aaagattaaa aatatttgct gagtcattac
    46261 taaaaattat agatacacaa attgaaataa aagaatatga agaaagcgag gattacaaga
    46321 tatttgaatt aatttcagaa gaacttgaaa gagatatacc tatagatgtt cagatataag
    46381 aaaagagcca ctgcaatggc tctaatcaaa aatatatcaa aaatttaatt agctatatta
    46441 tagcataaat ggagggaaat tatgagtact ttatacgaat taactacaga tttattagaa
    46501 atagaagaag gtttaacaga aacaacagga aatgaagctg aaaaactaga ggaaataaaa
    46561 gaaataataa aacaagagat acaaaataaa aacactagga tagtttcagt aatattaaac
    46621 attgacagtg atataaactc tatagattca gagattaaaa gattgcaaga gttaaaaagg
    46681 gccaaaaaga atactcttga taaattaaaa agcaatataa aagactgtat ggaattactt
    46741 gagactaaaa aagtagaaac aattttagga aatataagta taagaaagtc ggcaggtagc
    46801 ttagtcatag aagatgaaga aaagatacct gctatatata aaacagttga gcaagttgta
    46861 aaagtagata agaacactat taaagacttt attaaaaaag gtcatgaggt tgaaggttgt
    46921 aggattgaat atggaactac actaacaatt ccaaaagcta aaaaagagta ggtgaggacc
    46981 atggaaacta ataatgttta tataaaactt gtaaatatac agagtacttt aaaagctcct
    47041 aaaagtcaat ttaatagctt tggtaaatac aactatagga gttgtgagga tatactagaa
    47101 ggtttaaagc ctattctaaa agaagaaaaa gcattggttg tattggatga taatattgtt
    47161 cagataggaa atagatttta tgtagaagct acagcaactt taatagatgc agaaacagga
    47221 gaaaaaatat ctgtaaaagc attagctaga gaagatgaaa ctaaaaaagg tatggattta
    47281 gcacaagtaa ctggaagtgt atcaagttat gcaagaaagt atgctttaaa tggattattt
    47341 tgtattgatg atacaaaaga tagtgatgca acaaataaac atggaaatga gcagaaaaaa
    47401 aaagaagtta atgagagcga attaaatata ctatattcgc taggagaatc tatagaaaaa
    47461 gataaaaata gagttgatag tgaagtatat aagaagtttg gaaagttagc agtagatttg
    47521 actaagcagg agtatgagaa agttttaaat ggatataaga gcattttaga gaagcaaaaa
    47581 caagagtagg tgatagtatt ggggattata agagtaagca aagacaaaga taatccatat
    47641 gtagttttaa ataaaacttg tttggaagat gtaaaattaa gctggcaagc aaaaggctta
    47701 cattcatatc tgattagtaa gcccgaccat tggaaaatct atgttaatga cctatataaa
    47761 agaagtaaaa atggaaggga tgctacagca aatattttaa aagaacttat agaaaatgga
    47821 tatgtaacaa gaaaaccttg tagagattct aatactaata agatgcttgg aggatatgat
    47881 tatcaagtat atgagatacc acttgaaaat cctcagaagc taaaatcccg aaaaactgat
    47941 ttcccggaaa ccggatttcc cggaaaccgg gtttctcgga aaccggaaaa cacggaagta
    48001 gtaagtaatg actttaaagt aaataatgat attactacta ttgttattaa tgaacaatcc
    48061 aataaagata aaaccaccta cataaaaaaa tattatgaat cttatatagg tgtgattact
    48121 ccaaataatt ttcttcaact actaagttat ttagatgatg gaatggaagc tgatgtaata
    48181 ataagagctg ttgatgaagc tgtaggcagt ggagttaaga attataaata cattaaaaca
    48241 atattaaata attggataga agcaggtgta aaaactaatt tagaacttac agagtatcaa
    48301 aatgagtttg agaggaagaa aaagagtaaa caggagaaga aacagtctaa taataaaact
    48361 gtgaatactc ataatgtaag taaaaataag tttgctaact tcaatcagac ttttactcag
    48421 tatgatgaaa aagagctaga cgagattatt aaaaagagtc agaaggaaaa atttaaataa
    48481 aaattaaact tctaggaagt aaatatcaat atattgtttc ctagaaaggg gaggtataaa
    48541 atggcgagaa tatatgcaca aagaagtggt tctttaaatg agcaggatag attggaattg
    48601 ttaagattac ttgggaaagc tggatataca gtaaagattg gtagagagaa gcaaaatagc
    48661 aagacaactt atacttactt tgttgagtat acagaagagc aggaagaaaa atagaagggg
    48721 gctagttaaa tgaatacaat aactttagtt ggaagattag ttgcagatgc tgagctgaaa
    48781 tatcttccaa attcgggtac acctaaaatt ttatttaggg tagcagtaga tagaaaatac
    48841 aaagatagag aaggcaaaaa gaaagttgat tttatacctt gtgaacagtt aggaaaacat
    48901 gtagaaaatc ttgtacagta tcttacaaaa ggtaaacaga tatatgcttt tggagaatta
    48961 aatatagata attacaaaga tgaaaatggt tgttggaaat ctattactaa ggttaatgta
    49021 aatgctttag aattgctttc aagtaaagag gatagtaata atcatgaaaa acaacaggaa
    49081 tatataccac caggactgga cccacaaggt tttcaagcaa tagatgatga cgatatacct
    49141 ttttaatcaa gttaagtagt ctagggagta attatacaat attacttcct agaagttaaa
    49201 aaatggaggg atgaagatgg agttaaaatt tagagaatgg aataaaaatg gtaaagaaat
    49261 gtatagttat gatgaaatgg tgtgctattc taaaaatttg cttagagagt gggtttatag
    49321 tggtgtttat ttgccaaaaa acaatgaaaa ttttgaggtt atgatataca caggtttaaa
    49381 ggattgtgat ggaaaagaaa tctatgaagg cgatattgtt ttatgtagaa gtatattttt
    49441 tacagagttt gcaggggagg taaaatttaa agatggttgt tttattgcag tgaatgaaat
    49501 gtcaggagat tgctttagat tatctgaact tgcaatagtt aaggttgatg ggaatatata
    49561 tgaagattta agtaagttaa atgatataag aagatgtgat gaggggggca ttaggaatga
    49621 taataattag aagtcaagat agattagatt taatgagagt taacagagtt gaaataggtt
    49681 ttaatcaagt gtatgcaata cttgaaaatg atgttagaaa aataggtgaa tatgaaagta
    49741 atgaaagagc tatgcaagtg ttaaatgaaa tacagaagtt tattgaaaat ggagtaagaa
    49801 cggattatat agattcttgc agagttagac ataatcaaga gaaagtattt gaaatgccat
    49861 ttgaataggg aggggggaac taaatatggc taaagtttgg atggatgcag gagaattgtt
    49921 agaaaaaact attgatatag aagatatgtt tggatgtaat ctaagtaaaa tgagaaataa
    49981 aagtaaacaa aaaggtttag ttggtagaac aaattctaaa ggtcaaggaa gaaaaagtaa
    50041 gaaagtagag tgtactaaca ttattactgg agagagtaaa atatttgtca gtgctgtcga
    50101 agcaagtaaa tatttatatt ttacagaact tcatattgct cgtctagcaa gaacaggaaa
    50161 gactactaaa aatggttgga aagttagata tattcaagag gtgacagatg gtattggcaa
    50221 atgtggagca agtaattaaa ttggctgaaa agatattaaa taagaaaaaa tgttctgtta
    50281 ataaagctat tgatacagct ataaaaatat taagtagata tgagtatgag gggatgttag
    50341 aaaaatgaat atattagcta ttacaatgct tataatagga agttttatag ctggtagagt
    50401 ttatgagtat agattgaatc taaaagagtg tgaaaattgc gacaataaaa gaggtgtata
    50461 agaatggatg atagattaga aattataaat gcttctgtaa attatataca gatgatatgt
    50521 gaaagttcaa atatagctat tatagcagag cgaggaagag ttagaatatt agatttagaa
    50581 actaaagaaa aatatgattt attaaaaaat aaactcgagg aaatgttaga agaaatataa
    50641 gtgaaaatat ctaattaaga cagtttagag agttacaaaa tatcttttag tataaattat
    50701 tgttgaagtg ttttgt
//
LOCUS       DAMJWS010000019        47727 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMJWS010000019 DAMJWS010000000
VERSION     DAMJWS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.019"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            160..1311
                     /locus_tag="PC399_001181"
     CDS             160..1311
                     /locus_tag="PC399_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2592054.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1429..2067
                     /locus_tag="PC399_001182"
     CDS             1429..2067
                     /locus_tag="PC399_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2592055.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2068..3090
                     /locus_tag="PC399_001183"
     CDS             2068..3090
                     /locus_tag="PC399_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDF2592056.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3080..3700
                     /locus_tag="PC399_001184"
     CDS             3080..3700
                     /locus_tag="PC399_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592057.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3824..4510)
                     /locus_tag="PC399_001185"
     CDS             complement(3824..4510)
                     /locus_tag="PC399_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF2592058.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4524..5069)
                     /locus_tag="PC399_001186"
     CDS             complement(4524..5069)
                     /locus_tag="PC399_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2592059.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5297..6544
                     /locus_tag="PC399_001187"
     CDS             5297..6544
                     /locus_tag="PC399_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2592060.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6631..8625
                     /locus_tag="PC399_001188"
     CDS             6631..8625
                     /locus_tag="PC399_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2592061.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8625..9947
                     /locus_tag="PC399_001189"
     CDS             8625..9947
                     /locus_tag="PC399_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2592062.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10226..11512
                     /locus_tag="PC399_001190"
     CDS             10226..11512
                     /locus_tag="PC399_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2592063.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11598..12905
                     /gene="grdB"
                     /locus_tag="PC399_001191"
     CDS             11598..12905
                     /gene="grdB"
                     /locus_tag="PC399_001191"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12645..12647,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2592064.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13183..13914
                     /locus_tag="PC399_001192"
     CDS             13183..13914
                     /locus_tag="PC399_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2592065.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            13916..15274
                     /locus_tag="PC399_001193"
     CDS             13916..15274
                     /locus_tag="PC399_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2592066.1"
                     /translation="MINLFSSDLFLKYSFLFSFIVYRILMVIVQYYFLISFTNVNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            15313..15798
                     /locus_tag="PC399_001194"
     CDS             15313..15798
                     /locus_tag="PC399_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592067.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(16061..16297)
                     /locus_tag="PC399_001195"
     CDS             complement(16061..16297)
                     /locus_tag="PC399_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592068.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(16452..16676)
                     /locus_tag="PC399_001196"
     CDS             complement(16452..16676)
                     /locus_tag="PC399_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2592069.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(16773..18026)
                     /locus_tag="PC399_001197"
     CDS             complement(16773..18026)
                     /locus_tag="PC399_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF2592070.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            18286..19275
                     /locus_tag="PC399_001198"
     CDS             18286..19275
                     /locus_tag="PC399_001198"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF2592071.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            19347..20087
                     /locus_tag="PC399_001199"
     CDS             19347..20087
                     /locus_tag="PC399_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF2592072.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            20303..21454
                     /locus_tag="PC399_001200"
     CDS             20303..21454
                     /locus_tag="PC399_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2592073.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            21479..22234
                     /locus_tag="PC399_001201"
     CDS             21479..22234
                     /locus_tag="PC399_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF2592074.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            22426..24813
                     /locus_tag="PC399_001202"
     CDS             22426..24813
                     /locus_tag="PC399_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF2592075.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(25044..25277)
                     /locus_tag="PC399_001203"
     CDS             complement(25044..25277)
                     /locus_tag="PC399_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2592076.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            25566..26156
                     /locus_tag="PC399_001204"
     CDS             25566..26156
                     /locus_tag="PC399_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2592077.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            26270..27115
                     /locus_tag="PC399_001205"
     CDS             26270..27115
                     /locus_tag="PC399_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592078.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            27112..27795
                     /locus_tag="PC399_001206"
     CDS             27112..27795
                     /locus_tag="PC399_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2592079.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            27792..28508
                     /locus_tag="PC399_001207"
     CDS             27792..28508
                     /locus_tag="PC399_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2592080.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            28605..28868
                     /locus_tag="PC399_001208"
     CDS             28605..28868
                     /locus_tag="PC399_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592081.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            28840..29295
                     /gene="def"
                     /locus_tag="PC399_001209"
     CDS             28840..29295
                     /gene="def"
                     /locus_tag="PC399_001209"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2592082.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            29347..29808
                     /locus_tag="PC399_001210"
     CDS             29347..29808
                     /locus_tag="PC399_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF2592083.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            29858..30760
                     /locus_tag="PC399_001211"
     CDS             29858..30760
                     /locus_tag="PC399_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2592084.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            31130..31942
                     /locus_tag="PC399_001212"
     CDS             31130..31942
                     /locus_tag="PC399_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2592085.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            32990..33751
                     /locus_tag="PC399_001213"
     CDS             32990..33751
                     /locus_tag="PC399_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2592086.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            33816..34598
                     /locus_tag="PC399_001214"
     CDS             33816..34598
                     /locus_tag="PC399_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2592087.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            34865..35284
                     /locus_tag="PC399_001215"
     CDS             34865..35284
                     /locus_tag="PC399_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF2592088.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(35387..35653)
                     /locus_tag="PC399_001216"
     CDS             complement(35387..35653)
                     /locus_tag="PC399_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF2592089.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            35880..36581
                     /locus_tag="PC399_001217"
     CDS             35880..36581
                     /locus_tag="PC399_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592090.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            36663..37271
                     /locus_tag="PC399_001218"
     CDS             36663..37271
                     /locus_tag="PC399_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2592091.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            37595..38059
                     /locus_tag="PC399_001219"
     CDS             37595..38059
                     /locus_tag="PC399_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HDF2592092.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            38696..39712
                     /gene="gap"
                     /locus_tag="PC399_001220"
     CDS             38696..39712
                     /gene="gap"
                     /locus_tag="PC399_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2592093.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            40107..41276
                     /locus_tag="PC399_001221"
     CDS             40107..41276
                     /locus_tag="PC399_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592094.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            41656..45909
                     /locus_tag="PC399_001222"
     CDS             41656..45909
                     /locus_tag="PC399_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2592095.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            46058..46552
                     /locus_tag="PC399_001223"
     CDS             46058..46552
                     /locus_tag="PC399_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2592096.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            46668..47045
                     /locus_tag="PC399_001224"
     CDS             46668..47045
                     /locus_tag="PC399_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF2592097.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            47251..47691
                     /locus_tag="PC399_001225"
     CDS             47251..47691
                     /locus_tag="PC399_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF2592098.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
BASE COUNT        19198 a         4813 c         8066 g        15650 t
ORIGIN      
        1 aagtttaact ataaattagg tttatatttt ataaaaattt gctaaaaaaa acaattaaaa
       61 attgtaaaga aaaaagatat aattagaact gtagtaataa atgttttatt atagataata
      121 gagttttaag tatcttaaat aatatttgga ggaatatgaa tggaattaag accaagaagt
      181 gcaaaattag aagaatttga taaagtaata gaattaataa actatgtatt tagaatatca
      241 agaaatcata aaccaactat gatggaagaa tttcctcttt tactaagtaa aaataatata
      301 gaaaatatga ttataatatc agaagatgat aaagtggttt ctgatgtaaa ctatctaatt
      361 caggatgtat ctattcaagg aaacagattg aaggttgcag ctataggtgg agtatgtact
      421 catccagatt atgaaaagag aggctattca tctaagatat tggataaagt agaagaaaaa
      481 atgttttatg atggtgtaga tattgtaatt atatcaggaa caagaagtct atacagtaga
      541 agaaattgta gtttggtaaa gagtttttat aaatatacaa taaaacctga agatgtaaaa
      601 atagcatatg aaatagttga atttgatgaa actaattttg aaaaagataa tgatttagat
      661 aaaatgattg aactatataa tcaaaatagt actagattta taagaacgag agatgagttt
      721 caaaaactat tgcatgcagc aactattgcc tggggaccaa ttggttacaa gaaggtattt
      781 ataaaggaaa ataataatat aataggatat ttgataataa gaacaataaa aaaagaagat
      841 tctactgtag gagaagttgc tgaaataggg ttaaactctg taaatgttga aaatatattg
      901 aaatatgttg caaataaatt tggattagaa tctttaaatt ataaagtaca tgtgaaaaat
      961 cttaaagacc agttgaaatg taatgggaca aaatctttgg attatcaaca aggaactatg
     1021 aaaataataa attttactaa gttgtgtgat agtttaagaa gttattttag tcaatatgta
     1081 gattttgaat tactaaaata catggaattt aaacaagttg aaaataaata tattattaaa
     1141 tacaaagaag aagaacttgt aatagaaaat ttggataagc ttaataaact gttttttgaa
     1201 aaaaatgagg aacagtacaa tgaatttaaa catctaaaaa atatatatga atttgctaca
     1261 aaggcatttc ctgtagattt tccatggact gcaaatttaa attatcaata gttttagatg
     1321 atattaaata aaattgattg atacctagag ttagtccttg ttaaataaga gaaattattg
     1381 gatattgaaa aagcaatttt gtaagtaatt agaaaggatg agtattatat ggacatactt
     1441 gatgatatag cagaatgtat attaaatatg ggtgtagata atatagagaa tttagtaaaa
     1501 attgctatag ataaaaatat tgatgtggaa gatatttatg aatatggttt aaacaaaggt
     1561 atgataggag ctttagataa gtttgagaat aaggaatact atctttcaga agttatagta
     1621 tgcacagatg cattaaataa aggtattaat cttttgaagg gaactggaaa agtaaagaaa
     1681 aaatcaaagg gagtaattct gatgtctgta gtagaaggag atactcatga gataggaaaa
     1741 aatattgtaa aagtaatggt agaagccact ggatataagg ttattgactt aggtgtaaat
     1801 agaaaaagtg aagatataat agaagaagct ataaaaaaca atgtagatat aataggctta
     1861 tcttcaatga tgacaactac aatggaaaat atgaaatcgg ttatagacga acttaatatg
     1921 attgagatag ataaaagacc aaaagtaata attggtggag gacctgtaag tatggagttt
     1981 gcagaagaaa taggagcaga tggatacagt tcaaatgctc caaaagcagt taaattaata
     2041 aataaactta taggaggtga agtttaaatg ttttcagata tgactttact tcagtatata
     2101 aatagtgaag atagaggttt ttttctgcct gatatgggta caaatggatt gttcttaata
     2161 aataaaaagg catatgaagt atatgatagt gtagatttgc aattagaatt agcaaagact
     2221 atggacaagt attttgaatc agattttata tactcattct gtgatgggat tacattttgt
     2281 gaaactctgg gtttagatac attaagacca gattatgatt ttccaagtgt attgacacat
     2341 actataactg acagagataa attaagaaaa ttagatgttc cagaccctta tacgagtgga
     2401 cgtatgccac tcaatataga gagcttatca gttatatcaa aaagtattaa aaaaccacta
     2461 tatgaatcga tacaaggtcc atttacactt gcaggtcaat tagctggagc tactcaactt
     2521 ttaagatgta taataactga taaaaaattt gtagaagaat tattggaatt tacaactgaa
     2581 ttggtaagaa gatatgctgt agctgcaaat aaagcaggag caaaatatat ttcaatagca
     2641 gagccaacat cagtaaccct aagtaaagat agatttgatg aatttatagt aaaaaattta
     2701 aataaaatct acaatgaact tgattgttgg aaaggtatgc atatatgtgg agacaccaga
     2761 gaattattag ataatatgct aagttgtaat atagatgcag taagtttaga ccaaatttta
     2821 gactatgaag aaattgctcc aatgattcct agagatatag ttttgattgg taatttagac
     2881 ccaataaaac ttcttggacg ttctaagcca gataagatta gaagagagac actaaaattg
     2941 ttaaagaaga tgagaagata tgataacttt ttatgtgatt ttggctgtaa ctgcttaaat
     3001 accactccag tagaaaattt acaagcagct ataaaggcag gaagaattag ttataaagaa
     3061 ttagatagga ttgatattga tgaattataa tagtaataaa aataaatatc ttttaaagga
     3121 tataaattta gatgatattt ttatagataa gaatattgtg ctaaagtttt tggggtatgg
     3181 aaatagaaaa gctccagata caattagtaa aatcgttgaa agagaaataa gaaatataaa
     3241 tgatatttta gatatcaaga tatatataag tgaagttgat attaataaaa cacctaaaga
     3301 atgtaaaaaa gcatttgctg tactatatac aataggggat aaaattgatt ttaagatgaa
     3361 tgactatatg gaaaattgca atatgatggc agggctggca ttggataagg taggtatagt
     3421 atgtttagat tgtattaatg agaaaataaa aatgtattta aaaggaaagt atgatagtct
     3481 caaaatatcg catgaaatat atccaggaga taaagatttt gaagtagaga gacaaaagga
     3541 tatttataac tatgtaaaaa ataaatataa tagtatagaa atagaaatta atgattatca
     3601 tcaactatcc cccataaaaa gtgttgcaat gcttatttta atgggggatg aagaaaattt
     3661 agaatcaaga tgtagtaagt gtcctagaaa atgcttttag ttaattttca agactaagat
     3721 ttgttttgtg tataaattgc aataattttt ttggtcattt tatgtataaa ctaaaaaaat
     3781 tattgcaaag taatattcta tttaatttga acagacttac aaactatagt agtttcttta
     3841 attcaataag tgtatttatt tcataagttg gtatatgatt agtataattt atactgttat
     3901 tggaatttaa ccaacaagta tctattcctg aatttattcc acctaagata tcagaggtta
     3961 agctatcccc aattattaag gcagaagatt tatcatgaca attaaacttt ttgagagcgt
     4021 atttaaatat ttcaggattt ggttttgata ttttcaactc ttcagataca accattccat
     4081 caatatattc ttttattttt gaattttcaa gtcttttctt ttgaactgca gaaattccat
     4141 ttgttattat aacaatagta tacttttttt taagatattg taatatatcg attgcacctg
     4201 gaattaaaaa tgtacattca cctaaaaaat ttaagtacat tttgctaaga gtttcagaat
     4261 caacatccaa agatatttta tttgcaaaaa gttcaaatct aagtaattta agttcattca
     4321 aagttataat atttttttca aggtcgagcc aaagcttatc acttatattt ctatatgact
     4381 ctatataatt ctcaaaatta aattctaaat caaactcaga tagaagtttt ttaaaagcat
     4441 gtgattcact tttcttaaaa tcaaataaag tatcatctgc atcaaaaaat ataaatttat
     4501 atgtcttcat ggtttttact cctttacaag atgactgcaa aaattacttt actttcacta
     4561 tttgagtcat tttgccattt atgttttgtt cctaaaggaa cttttacact atctcctttg
     4621 gacaaagtaa attcatcatc atccataaaa agtttaactt ctccctccaa aacatatgca
     4681 atttcatctc cattatgagt aatcctatca acacaagaag aagtgtttgg agctaaatcc
     4741 ataattgcaa attctaaatt accactagag tctggaatta aaatctcata tataacttct
     4801 ttgcttccag gtaaaataac tttttttcta ttatcatgtc ttactatcaa gtttttttta
     4861 tctacctcag atacaaaaaa tgtaaataaa ggaacgttta gagaagaggc tatagatttt
     4921 aatgaattta cagatggatt agcaagacct cgttctattt ggctcaataa tgatggtgta
     4981 acatcagcta gttttgctaa atctcgaata cttaagtttt gtttttttcg aacttcagct
     5041 attttttccc ctaaatttaa atcattcata taaatatcct ctctttttaa tttaaaaaat
     5101 attaacttaa aataatcaag taaaatgatt atgtagtatg aaattattat aacatataga
     5161 attaggtttt gaaaaaacta tgttcaatat aattaaataa attaaaatat aattgacaaa
     5221 gtggatacag aatacttaaa atatatttaa ataaaattaa gcaaaagata gttaatttat
     5281 gataggaggg gatattatgc aaataacgac ttttggagct atatttggtt tattaatagc
     5341 aattatttta attataaaaa aatttcaagc tgtatatagt ttaatgctag gagcatttat
     5401 tggaggatta gttggaggag caaatataac acaaactgta gattttatgg caaatggagc
     5461 tatgaatata tcaccatcaa ttcttagagc tttggcatca ggtgtattag ctggaagttt
     5521 aataaaaaca ggggcagtag ataaaatatc agaacagatt gtaaaaatat ttggtgaaaa
     5581 gagggcttta ttttcgattg ccatttcaac tatggtactt gctggagtag gcgtaaattt
     5641 ggatgtatct ataataactg tagcacctat aggattatat ataggtagaa aattaaatta
     5701 ttctaaattg tcaatactat tagctatgtt gggagggggt aaagcaggta atataatatc
     5761 accaaatcca aatactatag cagtagcaga taatttttct gtcaatctct ctagtgttat
     5821 gatggcaaat ataattccag caattattgg agttgttatt acagttattt tagcaagtat
     5881 attaataaat aaaggaaata aagtacaatc atatgaaatt ttagagcaaa gagaagattt
     5941 accaagttta tttaaatcat tatgtggtcc aattatagct atatttctat tgtttttagg
     6001 aaatgtatct cctatagtta tagacccaat gatagccctt ccaataggag gaatcgtaac
     6061 tctaattgtc acaggaaact tgaacaattc aagagaatat ttagcttttg gattatctaa
     6121 gatgcaaggg gtatgcatat tattacttgg aacagggact atcgcaggaa ttatacagat
     6181 gtctgaatta caacagagta caataggtgc tttacaattt ttaaatatgc cacaattttt
     6241 attggcacca gtttcgggta tacttatgtc attagcaaca gcatcatcaa cagctggtgc
     6301 tactatagca tcatctactt ttcatgatgc tataataaat ggtgggctat caccgatttc
     6361 aggtgcctca atagtaaatg ctgggtctag tgtattcgag caattaccac atggctcact
     6421 atttcatact agtgcaggta gtataaacat ggatataggt gaaagattta agttaattcc
     6481 atatgaagca ttgataggaa tagtaatgac tataatttca acatctattc aattagtttt
     6541 ataataaata taaatattta atgaaaaaag taggattttg ttattaaata ttgaatttat
     6601 tatagtaaaa aagaagattg gagttttaaa gtgaataaaa aaaagattgt tattataggg
     6661 attatttatt catttttagt agtattttca cttacaaata tgtatgtaaa tatggagtat
     6721 aatctaaatg tatttgaata tattaagaag tcacttccat ttacagaaga agaaaaaaag
     6781 tggttagaaa aacacaagaa tttgatttac agctctgacc aaagttcacc tcctttgaga
     6841 tataaaggta aggaagatgg acagtataag ggaattattg ttgatttaat aaattctctg
     6901 tcaattcaaa ttggtagaga tttttatttt aaaccaaata attggtggaa agaatctttc
     6961 gtaaattcaa tagatgatag tataaagttt tttgacttga ttccatcaaa agaaagggca
     7021 aataaattta tatttactga cccaatatac actttgagtg caaatatatt gaaagataaa
     7081 aagtcccaag atataaatag ttacatggat ttaaagggga aaacagtagc aattccagaa
     7141 ggtgattatt ctataaattt tttaaagcaa aaaattcaag atataaacat tttgttgact
     7201 ccagatataa agactggagt caaccattta atgtctggaa aagtagacgc agttgttggt
     7261 gatgaaccag tattgcgata ctatataaat aattatggac tttcaaataa atacagtgtt
     7321 ttaagtaatc ctatatatac taaaaaagca gttcttgctg tacctaaaca atatgaagag
     7381 ctggttagta ttttaaataa aggcattttt aaacttcaga aaaatggagt gtataaagat
     7441 ttaaagaaaa aatggtattc tacgtataat gaagttgatg atatattgta tgagcgagga
     7501 attgtgccta gtatttatct ttttataggt ataattttaa tatcaattta tgttttttat
     7561 agctatacat atttgttaaa gatagaaatc aaaaggaaaa cagaacaggt aatagaaaat
     7621 aaaaaaactt tagaggcaac ttttaatagt attacagata taataatgct tgtagatgaa
     7681 aataacaata tagtagaatc gaacaaagta ctatatgact ttatgggtga aatgagttat
     7741 aagatagcag atttgattag catgataaaa ggtgttattg aaaatacctt ctctgaaaat
     7801 actaataaaa ctagtgaaat agaaatacac aataaaattt taaagataaa cacttttcct
     7861 gtagagtata aaaaaaataa tactgagtat atagtagttc ttattaaaga tataactaat
     7921 gataaaatag tagaggcgaa attacttaga gaaaacaaaa tgatatctat aggacaatta
     7981 gcttctgggg tagcacatga gataagaaat cctttgggta taataagaaa taattgctat
     8041 ctcttaaaag ataacgttac aatggaagaa gtaaatgatt gtgttaaatc aatagaaagt
     8101 aatgtagata gggcaagtaa tataataacc aacttgttaa atttcgcaag aatatcagat
     8161 gataatttag aacatataaa tataagaaat tttattgaaa atatagtgaa acttcaatat
     8221 aaaatgttac agttaaagaa tgttgaaata aaaattgact gtgaacataa tttagtatgc
     8281 tatataaatg gagaatcgtt aaaacatgta tttataaatt taatttctaa ttcaatagat
     8341 gcaatacatc aagatggaaa aattattata tactgttatg aaaaaaatta ttgtttattt
     8401 atagatttta aagataatgg tgaaggaata aaagaagatg ccctaaagga catatttaat
     8461 cccttctata caacaaaacc aattggtgag ggaactggtt taggtttata cattacttat
     8521 aatgagatta aaaagaataa tggagatata agtgttgaaa gtaaattggg ggttggaact
     8581 tgttttcata taaaaattcc attaaataaa gaggtgacta tataatgaaa atactattag
     8641 ttgatgatga gctagaatat ggagtggtaa tgaaaaaaat tctacaaaag aagggctatc
     8701 ttgtagatat agcactaagt ggtgaagaag ctataaatat aattaaaaaa gataaaaatt
     8761 atgatttggt tttgtctgat gttatgatga aaaatatgga tggagtacag ctattagata
     8821 gaattaaatc tattaataga gatatagagg ttatattagt tacaggatat ggaagtattg
     8881 aaaatgcagt tgatgctatg aaaagaggag ctttatctta ttttataaaa agtaatccca
     8941 ttgaaaattt attagaagaa gttgaaaagg taaaggcaag taaaatatct gtttctgtac
     9001 aaaaaaataa tctggaattt actttggaat ctaaaaatag agattttaat aatatcataa
     9061 aaatagcaaa aaaagctgct tgtaaagatg ttaatatatt aatcttgggg gaatctggtg
     9121 taggaaaaga tatattagct agatatatac atagcataag tccaagaaag aatgagatat
     9181 ttgtacctgt aaattgttgt tctttttcag aaaatctatt ggagtctgag ttgtttggtc
     9241 atgaaaaagg ttcttttaca ggagcagtag atagtagaaa aggtagattt gaactttcga
     9301 ataaaggaac tctatttttg gatgaaatag gagatatacc tctaaatgtt caggtaaaac
     9361 ttttgaggac acttgaggac aaatctatag agaggatagg aagtaataaa tctattaaag
     9421 ttgactttag gttaatttgt gctatgaata aagaaccaaa agtggaaata tcaaatggaa
     9481 atataagaga agattttttt tatagaataa gtacaataac aataactata ccaccgctaa
     9541 gaaagagaag agaagactta actactctta tagaattttt cttaaataag taccaaattg
     9601 aacatgataa aaagatacac tcgattgata aggaagtaaa ggatttttta ttaaattaca
     9661 attatccagg aaatataaga gaattaaaaa atataataaa tagacttgta gttttaagtg
     9721 aagaaggcaa tttatcaaaa gacaatttaa atttaatatc aaataatgta tacatagatg
     9781 ataaaataag tattagacct ttaagagaaa taagaaaaga gtttgaatgt gagtatattg
     9841 aaaaagtttt atctttgtgt ggaaataata tttcaaatac agctaaaaaa ttggagatta
     9901 gtagaagaca gttaaccaat aaaatatcag agtataatat taagtgagaa aaaaacttct
     9961 cattaagtga gaaaaaaatt tctcaaactg tattaaaaga taaacagaaa aaatctgttt
    10021 atcttttttt attataattt taaaaattaa tatttacaat aattattgct aaattcaata
    10081 ttggtgtaaa ttaaaaatga aaatttctat gacattaata catagaatat atattttaaa
    10141 taattggcat agaaaatgct ttatatatta ataaacgtaa taaatacttt taattgcgat
    10201 taaatatatt aagggggatt ttcatatgaa gctagagcta gggaatatat ttataaaaaa
    10261 tgttgaattt ggagaaaaga cagaagtaaa agatggagta ctatatatta gtagcgaaga
    10321 aatagaaaag atagtattag aagatgaaag gattgtaagt gtaaatgttg aacttgcaag
    10381 acctggcgaa tcaataagaa tagctccagt taaagatgta atagagccta gagttaaaat
    10441 tggagatgaa agcaaaatat ttcctggaat tataaataaa gtgaagactg taggaagtgg
    10501 aagaacccat gtacttttag gagcttgtgt tgtaacttgt ggaaatatag ttggatttca
    10561 agaaggcgta attgatatga gtggtcctac tgctaaatat acaccttttt caaaaacaaa
    10621 taatatatgt attgttataa aagcaaagga tggtatagat actcatgatt atgaagaagc
    10681 agctagaatt gcaggattaa aaataggtgc atatgtaggt gaggcaggaa gagaagttga
    10741 gccagatgaa gtcattgtat atgagacaaa gccattatta aaacaagtaa aagaatatcc
    10801 agacctgcca acagtagcat atgttcacat gttacaatca caaggacttt tacatgacac
    10861 ttattactat ggggtggatg ctaaacagat agtgcctaca tttatgtatc caactgaaat
    10921 aatggatgga gctataatat ctggaaactg tgtagcacca tgtgataagg ttacaacatt
    10981 ccatcattta aataatccag ttatacatga cttatataaa agacatggaa aagatttaaa
    11041 ctttatagga gttattttga ctaatgagaa cgtattctta gtcgataaag agagaagctc
    11101 tgatatggta gctaaattaa tagaattttt aggagttgat ggagttctag tgacagaaga
    11161 aggttatggg aatccagata ctgacctaat gatgaattgt agaaaatgta gtgaagttgg
    11221 agcaaatgta gttttgataa cggatgaatt tcctggaaaa gatggcaaga gtcaatctat
    11281 agcagacgct actaaggaag cagatgcagt tgtttcttgt ggtcaaggaa atctagtggt
    11341 tcatttccca gcaatggaaa aaattatagg aactttagat tatgttgaaa tgatgatagg
    11401 tgggtataaa ggatgcttaa atgaagacgg aagcatggat gcagagctac aaattataat
    11461 agcatcaact atagcaaatg gctataatca tttgacagca agatattatt aaataagtat
    11521 ttgaatataa agttctgttg atataatcat tggtctaatt gatgtaacaa ctataaagtg
    11581 atttggagga ttaagagatg agtaaaatca gagtggttca ttatataaac aatttctttg
    11641 ctggtatagg tggagaggaa aaagcagata ttccgccaga aaaaagggct ggtgtagtag
    11701 gacctggaat agcatttcag agtcaattta atgatgatgc agagataatc tcaactgtaa
    11761 tttgcggaga tacgtatttt ggtgaaaata ttgagagtgc aactcaagaa cttttaaaaa
    11821 tgataaaaga agaaagccct gatttattta ttgcaggccc agcatttaat gcaggtagat
    11881 atggagtagc ttgtgggact atttgtaagg cagttgaaga agagttaaat attcctgtaa
    11941 ttacaggcat gtataaagaa aatccaggtg tagatatgtt taaattagat ttgcatatag
    12001 tgtctacagg aaattcagca gcaagtctta gaaaggtagt tccaataatg acaaaactag
    12061 gtcttaaatt ggttagaggt gaggaaatag gacctcctga agaggaagga tatataatga
    12121 gaggaataag aaaaaatttc ttccatgaac ttagagggtc tgaaagagca attgatatgt
    12181 tggttaaaaa gatgaaaggg gaaagctttg aaactgagta tccaatgcct gaatttgata
    12241 gagttacacc tatgaaacct gttaaagatt tatctaagat aaaacttgcc ctagtaactt
    12301 ctggagggat agttccaatt gataatccag acaaaattga atcttcaagt gctactaaat
    12361 atggaattta cgatttaaca gatatggatt ctatgtcaaa taaagaattt acaactatac
    12421 atggggggta tgacagagca tatgtccttg aaaatccaaa tttagttgta ccactagatg
    12481 tagttagaga gcttgaaaaa gaaggtgtaa ttggagaatt agtagattat tttataacta
    12541 ctacaggaac aggaacgagt gttggcaatt ctaagagatt tggagaagag ttttctaaaa
    12601 aattgttaga agatgatgta gatgctgtaa ttttaacttc tacatgaggt acttgtactc
    12661 gttgtggagc aacgatggta aaagagattg agagagctgg tatagcagtt gttcatatat
    12721 gtacagtagt accaatatca cttacaatag gagctaatag aattgttcca gcagtaggga
    12781 taccatatcc acttggaaat cctatgttag gagaagagga aagtaaaaaa attagaaaga
    12841 atatagtttt aaaagcttta aatgcactaa cagaagatat agaagaacaa acagtatttg
    12901 aataataaaa atacaacaca acactgaaat aaaaaaagat atcaaaaaat aattttttaa
    12961 aattatttta cgatatcttt tttttattag gtaaattcga aatttgcatt ttttaaaatt
    13021 aaatttataa aagtgataaa ttagtttatt ataatattga tacacaaatt caaattagat
    13081 atactatgaa aagcgagatt attacatttt ggaacaaata ttcaacatat tagatcatat
    13141 taattgaaca gaaaaaatta actttatata ataattagaa taatgataag aaagaaggtg
    13201 tatatattga tttcaattgc catttgtgat gatgaatcct atatgctaaa tcatttaaag
    13261 gaaaatatag aattatacat gaagcagaag aatttattat ataatataga gctttttaat
    13321 aaagtagaag atttactatg caatcatgaa aagtttaata ttgtattttt agatatacag
    13381 atggaagaga ttaacggaat gcaggcagct agaaaattaa ggtcatatgg taatgagtgc
    13441 tttataattt tcgttacagt tttaaatgaa tttgtatttg atgcttttga agtagatgct
    13501 ataaattatc tgataaaacc tctatctaat gagaaactat ataaaacttt ggataaaata
    13561 gttacaaaac ttgatgataa tgaaagtaat tatatcacaa tacaaaaagg ttattcatat
    13621 tggaaattaa aacttgctga tattttatat tgtgaagtta taaaaagaaa gatttatgtt
    13681 catttaaaga aagagactgt agattattat gagactattg aaaatttaga gagaagttta
    13741 ccaacaaatt tttttaggtg tcatcgtagt tatataatca atttacaata tgtatatgga
    13801 tatgaaaatg gtaatgcagt tatggaaagt ggagatagaa tacctgtttc tagactacgt
    13861 caacaaatat tttcaaaagt tatgcttgag ttcatgaaaa ataggaggat ataagatgat
    13921 taatttattt tcttccgatt tatttttaaa gtacagtttt ttattttctt ttattgtata
    13981 tagaatttta atggtaatag tgcaatacta ttttctaatc agttttacaa atgttaattc
    14041 aaaattttgg tgttttattt tatattcgat aatttcaatt tttttaatca ttataaatac
    14101 atattttaat ttatataatt tattttcttc acagattcta ttttttctta tgactttttt
    14161 atttgtaaag tacataataa aacaatctac tgttttatct atagtatcaa ctttgttagt
    14221 atatttaatt gaacaattag tatatggatt tacagctcca ttaaattact tgatatttac
    14281 tagtgatata aatacagata tttataaatt tgatataaat atgggactat cttctttaat
    14341 tactattata atagctggat ttatatattg gtatagttct aaaaagttta atattaaggt
    14401 catgaacttt gataaatata tagcaatttt gatgattcca ttgttgctta taatattatt
    14461 tatgcaatca tttgaatata gttcaaatat aaatatagat acaagtcttg ggattgtaaa
    14521 gctgctatta aatacttcta taactgaaga aattcaagct tttctttttt ctataattgg
    14581 aactatatgt gtattttttt ctctatttac ctttaagaaa ctaattcaag ctctcgaaga
    14641 tgacaaggag agggcaatta tgaatcaaca gattcatgct caaaaaaact atattgaaga
    14701 agcgaaatca cgattatcac aaactatatc ctttagacat gattttaata atcatttagc
    14761 tattgtcaat ggattgttga aaaaagacca aattttaaaa gcacaagatt atttaaataa
    14821 gcttgaaaaa gtaacaagtg acctatcatt ttcttgtaat acacagaata ttgttattga
    14881 tgctcttttc aacaataaat taagtatagc aaaacaatta ggcatagagg tagattgtga
    14941 agtaattata ccccaaagca ctaacatgaa cgattttgac ctatgtatag tgtttgctaa
    15001 tgctattgac aatgcaatta aagcttgtag agttgtagat aaacaaaata ggtatttaaa
    15061 gctttccact aagttagagg gtggattttt tatgatagaa ataaaaaata gctataatcc
    15121 aataaataat tatacaagtg gctcaggtat aggtctttta aatataaaag aaattgctaa
    15181 aaaatatcaa ggggctataa gtatagataa gacatcagat tattttcaaa taaatgtatt
    15241 gctggttatt tcattacatt gcgctagtat ttgataacaa tttcattgaa aatagtattt
    15301 gtaagctata ttatgtactt gaatagagaa agtttgatat atagaaatga ggaggttact
    15361 aaaatgaaag ttggaaatat aaatggtcaa gcaccaatta caaatggttt agatagttgt
    15421 caaagtgagg tacaaggctt agaacaaaag aaaaaatcat tacaggatga aatagagaaa
    15481 gtaagaagtg ataaaagtat tgatggtgaa acagccaaat taaaaataga agctttaaaa
    15541 aaagaaatgg aagagattga aaataaaatt caagagttaa aaggaaataa agcaaaaaaa
    15601 acaaataaag aagaaaataa tgtttcagaa gtaaataata atctattaga gagaaaaaaa
    15661 gaaccttcta ataatgaaaa tgtaaaacaa ttatatgatg tttttgaaag aagcaatgta
    15721 gacgaacaat gggataatac ttatcgtgtt ctccaagatg ataaaaactt aaaagtaaaa
    15781 tttaatagac ctatatagtt tatttataaa aatagttaat attttaatta tcaccacttt
    15841 ttagaagaat acacacttta ttttaaagac ccacatattt atttacagtt agcacattta
    15901 tatgtggttt tataactatt atttacaagt ctatattgat aacttttaca tagtatataa
    15961 tgtttaaata cttattcaaa tttatctata cataaacaaa aaaagagatt ttaaaatctc
    16021 tttttagatt gttgacttat ggctttttga tttaataaaa tcaagaagcc gtttgaattt
    16081 tatctgttgg ctgataccct tctgatattg aaacaccatt tttgtttcca aaatcgttta
    16141 aaattaagaa cttattatga atgactattt cattatgaac aataagcaga taacatgaat
    16201 cttcaatcaa tgttttaaca gtagcattaa atgcttgctt atacagttct atcgtttgtt
    16261 tgaactgtcc attaaagtaa atatgagtca ataacataac tatatctaca caaattaata
    16321 taaatttttt atactgtaca ttatctaact gttgattatc tgtattacaa catgattgaa
    16381 tttaaaaagc catcaacaca tagaatgttg atggcctgaa aatagattta aaatctcttt
    16441 tttattatat attaattatt ttttaaattt atattttttg catcagattt tctaatagct
    16501 aagtcaaacc ctctaaatct aatcactata gggtcattaa gaggagctat ctttttcact
    16561 tcaacttcag tgccatctat tagtcctaag gctagaagtc tcttcataag tttttcattt
    16621 ccagctatat tgtctataat tcccttttgt ccaagtttta agttgtaaac agtcattatt
    16681 aacacctcct aatgaaaatc attatcaaac tttatattta taatatataa caaaaagagc
    16741 aaattgtcaa tatataacaa aaaataaaca tactaagtta gtatgtttat aaaaaatagt
    16801 atacatggga tactaatagc atctagcaaa aaaccactta ctaatggtac aactaaaaag
    16861 gcttttggag aatcaccata tttttgtgtc aaagaactca tatttgccag agcgttcgga
    16921 gtagctccta ttccatgtcc aatcaaacca cttattatga tggcagaatc aaaatctttt
    16981 ccaagtactc taaagcaaat aaatattgaa aataggatta taaagattac ttgaaaagtt
    17041 actattaaga gaataggagg taataatgta gataattcaa ataaatctat attcataagt
    17101 gataaagtta aaaaaattcc caatgaaagt tcttttaaaa aatctataag tttaaaattt
    17161 aattctagta tatttatctt atcatttaaa tttctaaaaa taacagccga aaacatacaa
    17221 gttacaacag agggtaatag tatattaaac gttatataga agcatctact tataatttct
    17281 cctgtattta tacaaataaa tatgagaaga acctgttcca aaaatacaga tggtgttatt
    17341 gtttcggaga agttattatt ttttatccta ttcttatttc taaaaaattt acttccaaat
    17401 ctctttagat ttctattata tctaggaatc gacaagtcta tatttgatga tggttttaat
    17461 ttatatttat ttattagaaa tttagctaca ggacatccca atataccacc cataataagt
    17521 cctattgttg cagcagataa tccaacccca actgctcctt ctataccgag attttctatt
    17581 gtaagtccaa atgcagcaga ataaccatga ccaccttcca tagatacatt tccacacata
    17641 agccctaaaa gaggttcaag ttttataatc tttgatattg taatagctaa tatattttga
    17701 caaaatgcca atactccaca caaaatccaa tatctaaata atacactacc acctttcttt
    17761 ataagattta tgcttatttc aagtcctata gttgtaaaaa agaaggttat gaaatttggc
    17821 attaaatttc cacttataga tatatctgct atattaaaat atttaaaaaa taaatttaga
    17881 aaacaaaata atagacctcc tattactggt gcaggtatac agaaattatt taaaaagtta
    17941 actttattct tcaataaatt accaattaaa aagaagaata tagatagtgt caaggtttgc
    18001 actatatcaa aacttaaggt aatcaaaata agtcctcctt tgtaattcaa agttttaatt
    18061 atataactta aattcttatg tattgaaatt ttattatcaa atgtatatta ataaatgtat
    18121 attaaaaaga atcagtagat ataattagat agtcataaat taaattaaaa tattttaatg
    18181 agatatgact tttttagaat taaaaaccgt agtatattga tataatagaa ataagactta
    18241 aaaatctgtt gatagtatta tttataagta cagtaggagg ataaaatgtt tgaaaattta
    18301 cctaagatag atttacattg ccacttagat ggaagtgtta gagtagaaac catgcttgat
    18361 atagctatta aagagaaaat agatttacct tcaaataata tggatgaaat aaaaaaatta
    18421 gcaaaggttt cttttaattg tacttcacta gatgaatatt tagaaaaatt tgatttaccc
    18481 ttaaaagtaa tgcaatctaa ggaaaattta aaaagaatta cttttgaact tttggaagat
    18541 gcaagtagag aaaatgtaaa atatattgaa attagatttg caccattact tcacacccaa
    18601 aaaggtatga gtgttaaaaa tataattgag ggtataattg aaggaattag agaagctgaa
    18661 agtatttatg atataaaagg caatttaata ttaggttgta tgagaacaat gacctcaaaa
    18721 gaggcattat tagtaattga agaagggaaa tcatttgtaa ataaaggtgt tgtagcagta
    18781 gacttgtgtg gtccagagaa agagggattt tgtaaggaat ataaagatgt ttttaaatta
    18841 gcaagagaat atggatataa agttacaata catgcaggag aagctgcaag tggagaaaat
    18901 gtattggatg ctataaatat tttaaaggcg gataggattg gtcatggggt aaaaattaaa
    18961 gaccataaaa aggcgtataa tttagttaaa gataaaaaaa tattgcttga attatgtcca
    19021 actagtaatg tacaaactaa gacagtagat tcttatgaag cacatccatt ttatactttt
    19081 tataaagata atttacatgt aagtataaat actgataata gaactgtatc agatataaat
    19141 ttaagttcag aactaaatgt tatatttgat acttttaaac taggattaga agactataaa
    19201 ataatttata gaaatgcagt agaagctagt tttgcagata aagaaactaa agagtatttg
    19261 aatagtttaa tttaatagat aaaaatattt aaactttaaa ttaagctatt aaattgataa
    19321 aaatacattg tattaggaga aaaaatgtga aagattatga agatgttgtt tatattaaac
    19381 tagtcaaaaa agaacttgaa gaatggaaga aaaatatatt aaagaaacct tctatatttg
    19441 acaaagcatc aaagggtata cagaataaat ttaatggagt attgccagat aaatatcatg
    19501 aaataattac ttcttctata aaagtaatga caaaaaccgt actgtttgga tataaatatg
    19561 ctacaaagcc accacttaga aatatgtcat tagaagaaag agaaaaatta gttttagaaa
    19621 aagctaaaac ttacaagact acagcaatga ttgaaggggc aggaacaggg gcaggaggaa
    19681 tcctcttagg tttatctgat tttcctttgc ttttaggtat taaagtgaaa tttttatatg
    19741 atgtagcttc aatatatgga tatgatgtta aagattatag agaaaggatt tatatattaa
    19801 atatattaca gttggctttt tctagtcaac agcatataaa caaaactttt aaattgatga
    19861 ataactggga tgaatatgta aatagtctac cagaagacat caatgatttt gattggagaa
    19921 catttcaaca agagtatagg gattatttag atttagctaa gtttttacaa ctaatgcctg
    19981 gaataggagc atttgtagga ttttatgtga atggaaaatt aattgataaa ttatgtgaaa
    20041 ctgctgtgtc tgcttataga ttgaggattc gagagcttaa gttataagaa aaattataac
    20101 ataaaaaatt agttatacaa tatatctata tgtatctaat tttgcattga taaaaacaat
    20161 tatggataaa aggtaaagca tgatagaatc taatgtagag gtttgtattt taatggaaaa
    20221 atttagtttt tattgcttaa gatacaaatt gaattacgaa gagattgaat tatgaagaga
    20281 agtaattggg aggaatattt tcatgacaaa tttgccaaaa caatttgata gcttcaatga
    20341 agctagacaa aaaggattta taaatgcaaa ggaacttaag gaaagtggga aaaagatggt
    20401 tggagttttc tgtacattta ctccagttga aattccaatg gcagctggag caactgtagt
    20461 aggtgtatgc ggcgttagtg aagagccaat acctgatgca gaaagggtgc ttccaagaaa
    20521 tctttgtcct ttaattaaat caagctatgg acatgcaata acagatacct gtccttattt
    20581 ctatttttct gatttgttga taggagaaac cacttgtgat ggtaagaaaa aaatgtatga
    20641 agaacttgca aaagttaagc caacatatgt aatgcatttg ccaaatactt caaaaggaga
    20701 atttgcctat aaactttgga aagatgaaat gataagatta aaagaagagg ttgagaaatc
    20761 tttaggtgta actataactg aagaagatat aagaactgct ataaaagata aaaatgaaga
    20821 aagagaactt ttaaaagagt tttatgcact tggtaaatta caaccatctg cacttacagg
    20881 ccttgagtta cataatgttt tatatcaagc aggttttaaa tttgaccgtg cagaacttaa
    20941 acattcttta agaaaagtta tagatgatat gaaagaaaga tatgaaaaag gtgaatgtcc
    21001 tgttcaaaaa gataagccaa gaatattgat aacaggttct cctattggtg gaataagcga
    21061 aaaaatagtt aaaactttag aagatgcagg ggcatcagtc gttgcatatg aactttgtgg
    21121 agctattaga tcaaacgatt tattggttga tgaagaaata gaagatgtat atgatgcttt
    21181 aacacaaaaa tatataaata taggttgttc ttgtatgatg aataatgaca atcgtattga
    21241 attattagat agaataatag atgaatataa tgtagatgct gtaatagatg tagttttaca
    21301 agcatgtcat acatttaata tagaaagtta tagaataaga gaatttgtta caaaagaaaa
    21361 aaataagcca tttatgtctc ttgaaactga ttattcaaaa tcagatactg aacaattaag
    21421 aactagattt gaagcgtttg tagagatgtt gtaggaataa ataaaagtag gaggaactat
    21481 gtttagtata ggagttgact cagggtcagt ggcaacaaag ggtgtgctat ttgatggtga
    21541 aaaaattatt aagaaaataa ttataccaac tggatggagc cctaaaagta catctaaaca
    21601 ggtctatgag ttgctaagta gtgaaattga caaaaaagat ataaaaaagg tggtaggtac
    21661 aggttatgga aggggagtaa tggattttgc tgataagaaa gtaacagaaa taacttgtca
    21721 tactagaggt atttactttt taaacaagaa cataaggact attttagatg taggtggtca
    21781 agatagtaaa gttataaatt tagatagaga tggaaatgta tttaacttta taatgaatga
    21841 taagtgtgct gcaggaactg gtcgattttt agagattact tctaaccttt taggaagcga
    21901 tattgaaagt atagatacat tagctaaagg atatgaacca gttaatatat ctagtatgtg
    21961 tacagttttt gctgagtctg aaattgtaag tcttctagct caaaacattt ctactggaga
    22021 ggtagcagca gggatactta aatctattgc aaataaatcc acctcaatgt tagcaagagg
    22081 tgaggtaata gatgaagttg catttactgg agggcttgca aaaagtaaag aactagtaaa
    22141 aatgatagaa gaaatactgg gtaaaaaaat ttttattgca gaagatactc aaataatagg
    22201 agctttagga gctgctgtaa taggatttag ataaaaacat ataaaaagaa aatgagaagc
    22261 tgaatttatt ataaataaat tcagtttttt attttatttt atttatttat aaaggaaaac
    22321 tttggtataa ttgatttaaa tgattgtata tttgtaaata cttgatattt agtcatatat
    22381 aacattataa ttttaaaaat tagctcaaaa taagggggag aaagcgtgaa aaaatttact
    22441 tcaaaaaaag taacaaggtt tgttgcgctg tttttaatat ctattttagt aatgtcaaca
    22501 attcctgtat cagctgacaa tagcaataca ttgaatcaga taaaaaaagc agaatacagt
    22561 gagacgtata aacaatatct aaatgatgct aaaaatggat gtacagaaaa atataatggg
    22621 attataccaa atccatatca gttggaaggt actcaaatcc aaagtaaagg aagaacagct
    22681 ccaacatctt atgaccctag aaaattaggt cttatgacat caataaaaaa tcaagaggat
    22741 ttaggaatct gttgggattt tgctgcaatg gcaaccttag aaagtttctt aaagttaaat
    22801 aattatggag actatgattt atcagaagaa cacttaagat ggtgggcttc agatggtgaa
    22861 tatggatggt ctgtaaatga tatgaatggt gctttaaatt atgaagctat gggatattta
    22921 acatcttggt caggtcctaa attagaaaaa gatatcccat acaatggtag agtatctaaa
    22981 gcacaaggag caaaaaaacc aactaatatg aatacagcac ctactgtttt taatgtgaca
    23041 gatgctgtgt gtgtaagcaa tgacataaat tctacaaaaa atgctatatt acagtatgga
    23101 gctgtaacat ctggttatta tgaagatatt aaatatcaaa gtgatgacca aaattcatat
    23161 tattgtccaa caaaaagttt taatacaaat catgctattt caattgtagg ttgggatgat
    23221 aactattcta aagataattt taatgcaaat attagaccat caaaaaatgg tgcttggtta
    23281 ataaaaaata gctggggaga ttataattct gagggtggat atttctggat atcttatgaa
    23341 gacaagacac ttatgtctga tatagataat ttttcaatca aaggtggtaa aaaaccaaat
    23401 gatgatgaga agatgtatca acatgactat gcaagtattg tacctttaat ttcaaaaaag
    23461 ataacagctg caaatgtatt tgatttcaat agaggcgatg aaactctaaa atctgtaatg
    23521 ttcttaactg aaagtatagg agctaagtat gaagtatatt atgctccagt agtaaatggt
    23581 atacctcaag aaaacaatat gaaaaaatta aaagaaggta ctgcccaata ttcaggatac
    23641 ataacagttc caattgattc ttttgatata ccagaaggaa agggtgcaat tgtagtcagt
    23701 atagagggga aaaatggaga gtcaactata ggttctgagt caaatgtacc aggatatgat
    23761 acatttaaag caaaagctaa tttaggtgaa agttatatca tagataatac tggaaaattc
    23821 tttgatataa atagagatag taacttctat ccgtgtaatt ttactataaa agctgttaca
    23881 gaaaagtctt ctggtgagag tatcccaaat gagtctttaa ttggtagtga tagatatgaa
    23941 actgctatca aagttagtca aaatggtttc aattcttcag aaaatgtagt tttagtaaat
    24001 ggcagttcaa tagtagatgc attggcagca actcctttta catctgctat aaattcacca
    24061 atattattga cacaaaaaga agctttaaat tcaaaaacta aagctgagat acagagatta
    24121 ggtgctaaga aagtatacct aataggtgga gaaaattcta taagtaaaga aattgaacaa
    24181 caattaaaaa gcttaaatat atcgatagag agaatatcag gaagtgatag atacaagaca
    24241 agtttattat tagctcaaaa gttaaatggt ataaaaaatg tatctcaaat agcagttgta
    24301 aatggagtga aaggattagc tgatgcaatt agtgtaggcg ctgcagcagc agaaaataac
    24361 ataccaatca tacttgcaaa tgaaaagagc gaattgcaag gagctgatga atttctaaat
    24421 tcattaaata tagagaaatc ttatattatt ggaggaacag catccctatc aaacaatcta
    24481 gaaagtaagt taaaaaatcc aactagatta tctggtagta gtagagacga aactaattca
    24541 aagataatag ataattttta taaaaaagat actttaaaaa atgcatttgt agttaaaaat
    24601 ggaatcaaga atcaaaatga tttaatagat ggattggcag ttggtccttt aggagcaaaa
    24661 actgggtcac cagtaatttt agttggtgat aaattagctg atagccaaaa agaagttctt
    24721 aaaaataaga ctttagaaaa agtaactcaa gttggtggag gagcaaataa aaatgcattt
    24781 aaagagttag taaaattaaa aagtgctaaa tagagtactt aagtattaac atatattagt
    24841 gtgtattagt aacttatctt aataaaagct gaaaataatt acttcaaatt attgataaat
    24901 aagataatca atgattgtaa agttttgttt tcagctttaa atttatgtag tataagttta
    24961 aaaacataaa aaagggttac tttatataga aatatagaaa atcttacatt ttatatataa
    25021 ataacccata tttaattaac tttttatagt gttctaatag atattattga acttaatggt
    25081 aaatataatg tgtaagtttc atttaaagtt ggattagatt catctatata tcttgagcaa
    25141 agtgtaattg ataagctatc tgcttctata atttcaccat atagaggaca actgctaaag
    25201 taagaatgta cttcaacata tttatttaag ttttcttgaa cataatcgta aaaacggttt
    25261 tttgtagtag tagccataaa atcaactcct ttatttttta ttaaagttga aacttttaaa
    25321 aagataaata tgattacttc tattagaaac taagttaaaa aataagcaaa tagatatgaa
    25381 aattagaata atatattatt tatatcttaa ttataacact agataataaa aaatgctata
    25441 taaaattcag aattttcgaa agtttttaag cttattttta aatatactaa taatactatt
    25501 gacattagat agaaaaagta ttatattgac tatataaatt attttagtca aactggaggg
    25561 aaaagatgcc taaatcatat tctgataaag aaagagagta tataataaaa agacttaaag
    25621 aagaagcaag attgtgtatg gagcaatatg gtattagaaa aactactgtt gatgagcttg
    25681 taaaacgggt aaaaatacca aaaggaactt tttatctatt ttttcaatca aaagaattac
    25741 tcttttttga ggtattaaga gatatacatg actctataca aaaagaaatc ttatatgaga
    25801 taaatagagt agatgaaagt attacatgtg aacaattgac agatatattt atgaaattct
    25861 ataggatggt tgatagtact tcaattttaa atctcatgat aaatggagaa tttgagatat
    25921 tggtgagaaa gttaccagat tctataattg aagaatactt tagacatgat gactttgaaa
    25981 ttggagagat tatatcttgc ataccaaatg caaaaaataa agacatagaa agctttagtg
    26041 gagcatttag agcagttttt cttaccatgc tatataagcg cgaggttgga agtaattgtt
    26101 ttgaagatgc tttaagatta atgataaacg gattagttat acaacttatg gaatagaatt
    26161 taaattttgt ttgctaataa cttttattaa aaattattaa caaacaaaaa aattatattt
    26221 tattgaccat atagtcaaaa tagtcaaaaa tttgaaaggg tgatattgaa tgattaaagt
    26281 tgatgattta tcatttagtt atacagatag agatttttta caaaatatta actttgaagt
    26341 aggaaaaggg gaaatactag gatttttagg accatcaggt gcaggaaaat ctacattaca
    26401 aaagattcta attggaatga taactaatta tggaggaagt gtcattgtaa atggagtaga
    26461 gagtaaaaga cactcaaata aattttatga aaatatagga gttgattttg aatttcctag
    26521 tttatatgaa aagttgactg caatagaaaa tttaaaatat tttgggtctc tatattctaa
    26581 aaaactacta tctattgatg agttattaaa atcagtagga ctagaaaatg aatcaaataa
    26641 aagggtatct gagtattcaa aaggtatgaa atctcgatta aattttataa aagctttatt
    26701 acataatcca gatattttat ttttagatga accaacaagt ggtcttgacc catcaaatag
    26761 caaggttatg aaagatataa ttttatcaga aaaatcgaaa ggtaagacta ttattttaac
    26821 aactcataac atgttagatg caacagagct ttgtgacaga gtagcgttca ttgtaaatgg
    26881 taaaatatct gctttagata caccacataa tcttattatg tcaaaaggag ctattaaagt
    26941 tagatatact tactttgata atggagaaaa aacttctgaa tgttttctga acaatactgc
    27001 taatgataaa aatttgaata tgcttattga gaaaaataaa cttttatcaa tacatagtag
    27061 tgaaccaaca ttaaatgata tttttattga gataacaggg aggaatttac aatgaggcta
    27121 aaaaatctta ttttaggaga tataaagttt caatttaagt atggttttta ttttttatat
    27181 ctttttttaa gtattatata tatttgtatt atcaatgtat ttccaacttt tatgagagaa
    27241 aaaatagcta taattatgat ttattctgac ccagcagcaa tgggattgtt ttttatggga
    27301 gctattgtac ttcttgagaa aagtcaaagg gttttaaatt ccttagctgt atctccagta
    27361 aaagtatctg agtatatttt atcaaaggta atttcattag gagtaatatc tagtattgta
    27421 gctatgttta ttgctattac attaaattta gataatatta taatatcaac tataggtacc
    27481 tttttttctt ctattatatt ttctttgctt ggattgataa ttgcaagtaa agcatctagt
    27541 ttgaatcagt ttattgtttt aagtattcct attgagatta tatgctttat tccaccaata
    27601 ctgaatgtac ttcttgacac aaaatcatat gcaaatttgt acccttttaa tatatgtatt
    27661 tctcttatta gtggagataa aaattttata atgattaata ttttaatttt aatatcaatt
    27721 ataataataa tttattttat aacttattat tttatttgta gcagttggaa aaaggtaggt
    27781 ggagtaaaac tatgataaag attataaatg cgtttaaaca agagtttgaa caaattaaga
    27841 gagatgctat gctttttata gtatgtgttt ctccaatatt atgtggagta tttataaaat
    27901 ttggaatacc attaatacag aatatatcac ttaacaagtt ttattatcaa ttaaatttag
    27961 agccgtattt cttgatgttt gatttacttt tagcttttat tacacctttt atgttctttt
    28021 ttgcttctac aatggtcata ttgggagaaa ttgatgacag catttcaaga tatttaatag
    28081 taactccgct tggtaaaact ggttatctta tttcacgatt tggaatacca ggtatcttag
    28141 catttattat tactatgatt ctacttatct ttttttctct aactaaaatt tctctcttgt
    28201 taaacttatc aatttcttta ttagttttat tacaaggagt tattatatca atattagtga
    28261 tttctttatc atcaaataaa ctagaaggaa tggtaattac gaaattctca ggtgttttta
    28321 tgatgggtat tttagcacca ttttttattt taaataaagc acaatatatt ctgttcttct
    28381 taccaacttt ttggatatcg aaggcattta aggaagataa ttatgtctat atgtttattt
    28441 caattgttat atcattaata tggattttat tattatttaa aaagtttagt aaaaaaatag
    28501 ttaaataaga aaatttaata tataaatgta aggttgactt gacagtaatg ttgaaaaatg
    28561 ttacatttaa gatgtaaagt taaccttaca tgaatggagc aaatatgaaa aataaaataa
    28621 agatattaag agaaaaatta ggattgacac aagaacaact agggagatta gttggaacat
    28681 ctagacaggc tataaatgct atagaaactg gtaaaaatga accttcaata tggttggcat
    28741 atgatatttc aagaattttt aatgaaccaa ttgaaagtgt atttttattt gaagaaagtg
    28801 aaagaaaatc aagagcacag ataagtaggg gagaatatta tggctataag aaagataaga
    28861 acatttgatg atgaaatttt aagaaaaaag agtaagtatg tagaaaacgt agataataag
    28921 ataagagaaa tattaaatga catggcagag accatgtaca atactccaaa tggaggagga
    28981 ttagctgcat gccaagtagg agtattaaag cgattagttg ttatagattt aggagaagga
    29041 cttattaagc ttgttaatcc tgaaattatt gaacaagaag gggaacaaat tgttgttgaa
    29101 ggatgtttaa gttttcctga agtatggggt aaattaaaaa gaccaaaaaa agttacagtg
    29161 caagctctaa acgaatacgg tgagaaaata gaaattaaag gttcaggttt tatggcaaaa
    29221 tgtctttgtc atgagataga ccatttaaat ggtatagttt ttacagataa aattattgaa
    29281 catgtaaaat tgtaagatag attggttatt gaatttataa aatattttat agtaaggagt
    29341 taaattatga gattatggca taaggattta attgatgtat tacctaaaaa tcaattagta
    29401 agtcaatgga gagagctact tgctataaaa ggttctatag ataaaaaagg tactccaaac
    29461 catctattag tgaataaagt attgaattat agtatagatg aatttaaatt ttatacaaaa
    29521 attgtacatg atgaaatgtt aaaaagaaat tataaaccta atgaacttaa atatacaagt
    29581 atattaaaat ggaaaaatag aaattttgca aatgatatat ctaatgaaca tagtttaaat
    29641 ttagaaaatt tatatgatga ttggcataat aaaatgtatt taaagcaatg cttatataat
    29701 ttagaagaaa aagcaacatg tggaggaata ccaataaatg aatggaatat attactttgt
    29761 aaatatagta aagattatga attgtggtct ggaaatatca tgttttaatt actatagatt
    29821 aacattatag tattattgta tataaagagg tgattatttg tataataaaa taaaatatat
    29881 tccaataaat tgtgaaatag cttgtaataa attaaaaagg agtatgccat ataaatggga
    29941 tttaaatatt tatcgtggat gtgagcatgg atgtaaatat tgttatgcta tttactctca
    30001 taagtacatc aactccaata attattttga agagatttat gtaaaaacaa atatagtaga
    30061 aaaacttgag agacaactta aaagcagtaa atggaagaaa gaagtaatca atataggagg
    30121 agttacagat agttatcaac caatagaagc tgactataaa attatgccag aaatattaaa
    30181 cctgctaata aaatacaaga caccagctat tatttctaca aagtcagatt taattttaag
    30241 agattacgat ttaattgata agctatctag gattacatat atcaatgtag catctacaat
    30301 taccacagta aatgaagata cacaaaggct tattgagcca aatggtgtgg attcaatgag
    30361 acgatttaaa atgttaaaag aatttagaaa aactaacgca tcagttggtc tacatattat
    30421 gccaattata ccatatatta cagatgattt tgataatata aattcgctat ttagacatgc
    30481 aaaagaatgt aatgtacatt atgttttacc tgggacttta tacttaaggg gtataactag
    30541 aggtgttttt tttgactttg ttaaaaaaga gtttcctgaa ttatttgata aattatcaat
    30601 actatatagt gcaggttcac ttaataggga gtataaaaat caattgtata aaatggttaa
    30661 tgaacttaga tataagtatt ctctatcaag cagttactct aaaataatga aggaaaagtt
    30721 aaaaaactca ggagatgtac agatatcttt ttttgattag ttttatataa aaaaattttt
    30781 tttactatga tgattaaaat ttattaaaat taaaatttat tgacaaatat aaaatttaaa
    30841 tatataatta attcataaaa taaaaatatg aaaggatgat gttgttcggt gatatttgat
    30901 atcataaaat cgttgatata agtattgctg gaaaagagtg aataaaatca aatttaataa
    30961 gcaaacaaag taaagctata gttttattgt tgtgcttaaa aatatttggt tctattttac
    31021 tcttatttaa actcatcttt ttgaagttaa accacagagt aaactctgtg gtttttttat
    31081 tttaaaaata aattttaagg agtggtatgt tttgttaaac aaattatgta tgtatttaga
    31141 aagaccagag ctttataaac aaagtgaaat taatttttgg gatgatgagt atatttcaaa
    31201 gcaattatta aaagcacact tagacactaa ttttgaagga gcaagtagaa attttaactt
    31261 tatcgaggat tctgtaaatt ggatagttac agttgcaaat ccagctaatt atcctaaatt
    31321 acttgattta ggttgtggtc caggtttata tgcagagaaa tttgcacaaa aaggttataa
    31381 ggtaactgga attgatttct caaaacgttc tattaactat gcacaaaata gaaataagga
    31441 gactaattta aacataaatt acttgttcca aagttattta aatatgaact ataatgagga
    31501 atttgatttg gctactttaa tttattgtga ttatggagct ttgtcaactg aaaatagaag
    31561 gttattaatg gaaaaaattt atgatagttt aaaacctgga ggaaaattaa tattagatgt
    31621 atttacaata aataaataca ataattttga ggaaattaaa tattgggaaa taaatgaaga
    31681 tggaggattt tggagtaatg aaaaatatat gtgtctacaa gataattgta aatacaatga
    31741 ttataataca ttagaacaaa ctcttgtaat aaccgaaaaa gatgaaaatg tattttatgt
    31801 ttggaatcac tatttttcaa aagaaagttt cttattagaa gttgaaaata ttggatttaa
    31861 gtctgttgag ttttttagta atgttaaggg tgaagcatat tctgacgata gtatgactat
    31921 gggattagtt ttacaaaaat aataattttt ttaaaatgaa taaataaaaa cttgaaacct
    31981 ttaacaataa aataacataa taaaagctta catattgaaa aatggaaact tttatgttat
    32041 ttttagtaca ttacaaattt gcttatataa aaatcaatac ttgaattatt atttttatgt
    32101 gaaatttatc tatagtatat ttttataaac aagtaatgtt attaaaatta agtatgtagt
    32161 taaaagtgta tagcaaaaag gctgttatat taatgttaga ggttcaggga aaatatataa
    32221 agcaaagtat ttacagataa tgtagattgc acagctataa gttagataat agaattatgt
    32281 aatcaagaat ttacaacaaa gtataagata aggataatgc taaattgcca tacaggtgct
    32341 agatgtgtaa atggatttaa ttaatactat tgataaagtg caacaagcac ataaaccaat
    32401 tgatgagatt attaaaacta taagagatat agttgacata attaaaatat tggaacttgt
    32461 ttataatttt aaatcaaatc aataatgatt aagattaaaa taatattaag gcatcaatgt
    32521 tataagagta caatataagg agtactatat agtactcctt acttatacat attaggcaat
    32581 aggaaatttt ttcatagtta aattttcaag tattttttag gaaactttat aattgagaat
    32641 taagggtata gtatgtgatg aacttactga atttataatt aggttatata atggaataat
    32701 catcaatagt tttactttag ggaaaatata tgcttaagga agtatagctt actaatatta
    32761 ttaaaatgta tatttaaaat gataaaaaaa gaattagaca agtatttagt aaattgacta
    32821 tgcaaaattc tatcaagcag gaaccctatg actttagtca tgggaggttc agtaggtaac
    32881 ttgttgaatt aagtttatat tatttaaata aactacttaa tttagataat ataaaaagtc
    32941 agaattttaa gaatagagct atataatctt agctattagg agaattacta tgataggaat
    33001 atattttagt ggaacaggaa attcaagata ctgtgtagaa aaatttttac aagaatatga
    33061 tataacagca aattcttatt ctattgaaga taatgaactt ttacagcaca tcaataatca
    33121 tgagaatatt atttttagtt atccggtgca atatagtaat gtgccaaaaa tattgaaaga
    33181 ttttataata aatcattcaa ctttgtggaa tggtaaacgt gtttttatta ttgcaactat
    33241 ggctctattt agtggagatg gctctggtgt gttatctaga atattaaaaa aatacggtgc
    33301 tataactgtt ggtggattac atgtgaagat gccagatagt ataggagatg aaaaggtatt
    33361 aaaacattct ttagagtata atagaaagct tgtaataaaa gcagaaaaga aagtaactaa
    33421 agctgctaaa aaactgaaac atgggtcacc accaaaagaa gggttaggtt ttttgagtca
    33481 tattgtaggg ttatttggac aacgattgta ttttatcaac aaaacaaata tgtatacaga
    33541 taaactaaaa attaatactg aggaatgtat tgtatgtggt aaatgtgtta atttatgtcc
    33601 aatgaagaat ctagtaatta aaaattgtat gattgttgca aatgggcaat gtacaatgtg
    33661 ttatcgttgt gtaaataatt gtgctaaaaa agcaattaca ttgttaggta aaaaagttgt
    33721 tgaacagagt aatattaaaa aatatatgta agaaatttaa agttttattg aatgtaaatt
    33781 gatgtatatt ttatgttaga aagagaggtt aaataatgga atactacata gttgattcat
    33841 ttgcaactaa actatttaaa ggaaatcctg ctggagtttg tgttcttgac agaagaatac
    33901 ctttagagtt aatgcagaaa atagcagaag aaaacaattt accagaaaca gcatttgtag
    33961 ttaaaggtaa aggaaattat gaattacgat ggtttactcc taaagcagaa attgatttat
    34021 gtggtcatgc aactttagca gcagcttatg taatatcaaa ttttattgat gttaatgtaa
    34081 aaaggataga tttctttact caaagtggaa agcttgaagt tacacgaaat ggaaatctat
    34141 atgaaatgat atttccagaa ataatgccta ttgaaataga gttatcacca caacaagcta
    34201 atttgatagg ctgtgtacca agtgctgttt attcttcaag agatttaatc ttattgctaa
    34261 acagtgaaca agaagttata aattataaac ctaattacgc acaattgcgt aaattgacag
    34321 actggttagg tataataatc acagcacaag gaagtaatac agattttgta tcaagatatt
    34381 tttgtccaga attagattca gaagaccctg ttactggttc atcacattgt aatcttattc
    34441 catactggtc tgaaaagttg ggaaaacaca aaatggtagc tgctcaactt tctaatcgtg
    34501 gtggtataat tcagtgtgaa gtgttgaagg ataatactgt gaaaatttct ggtgaagcag
    34561 tcttgtttat gcagggaaca ataaagattg atatttaaat aagtataata actaatttat
    34621 tatgaaaaat aattaatttt cttagtgtta acaataataa agtagagtta aaaaacttga
    34681 tttcacttaa atcaatatga tagatgttga tactaagatt gacaagcatg attaaaaaag
    34741 ataatattta atctataaag ataaaaacac agtctggttg gtagtccaga cgcagcaata
    34801 gctgtcagta acctgcctcc ttggttgtcc gttctttaga aagtaattta aggaggaact
    34861 agcaatggac aaaattagtg gaaaattaac agtcttattt gaagaaccat tttgggtagg
    34921 tatatttgaa agacaagatg gtaaaaaata tgaagcatgt agggtagttt ttggagcaga
    34981 acctaaagaa gtagaagtgt atgaatttat acttgaaaga tttttctcat tagattttgg
    35041 tagcattaaa ttagaaaaaa atgtgaccaa agacaacatt ggatacaaaa gaatgcagag
    35101 aaaagtaaaa aaggaacaag aaaaagaaac tatagggact aaggctcaaa atgctcttaa
    35161 gcttcaatat gaagaaagaa aacaggatag aaaaaaccta gctaaaaata gaaaagaaga
    35221 agaaaaagaa agactattta atttgaaaca agaaaagaga aaagctaaac ataagggtca
    35281 ttaattttaa ttaaatatct ttttaataat tgctagaaaa aggtgtataa aatttttaat
    35341 tttatacacc ttaatttatt gaaaagaata aggttttata tttcaattac tttaaaatat
    35401 aaaaatatac taataaaaat tgtaaaaata gtataaatgg tataccaaat gtaaatttaa
    35461 tgtgtttagt tttatgtcta aaagaataca ttccaattat agaacctata ctacctccaa
    35521 ttactgctat agacataaga gtagcttctt ttattctcca ttcgtttcta attgctcttt
    35581 tcttatctat gtacattgaa aagaaagcaa tagaattaat cactataaaa tatattagaa
    35641 taaaattttt cacagtcttg cctccataaa aatatattat acattataaa acaagatatt
    35701 tgttttggaa agttaaattt gtaattagta catataaaat atttgataag aacaaatata
    35761 atattaattg gaaaatttaa aaaaattaaa caattagcaa atagggggta tatttataag
    35821 acacaaagtg ataaaatcta tttagaaaat aatgtaaaat aacaggagga taaagcaaaa
    35881 tgaaatttat agtagaaaaa gaagtttttg ataaattaga aaatgtatgc tttggtgtag
    35941 tagttgcaaa aggaatagat aatactaaag aaatagaaag aataagcaac cttttagata
    36001 taagtataga tagagtagaa gattacttca aagataaaaa agtaaaggaa tcagaagaaa
    36061 ttataccata tagagaggca tttagaagtc taggcatgaa cccaaataaa tttatgagtt
    36121 cgatagaagc aatgacaaca agggttgcta agaataagaa acttcctcat ataaatccta
    36181 ttgtagattt aggaaactct atttctttaa aatatttact tccaatgggt gctcatgata
    36241 tggattttag aaatgatgat gtatatgtta gattttctaa aaaaggggat aaatttgtac
    36301 catttggaga aactgatgtt gaattaatgg aagaaggaga attaatttat tcagttggag
    36361 acatggtaaa aactagaaga tggatatgga gacaaggaga agaaggaaaa attactaata
    36421 gctcaaaaaa tatatttttt cctatagatg gttttactga tgctaattta gataaggtaa
    36481 tgagtgcaag agaagaatta gcaaaattgt taaaggaaat atttaattgt gaaattaaag
    36541 taggttttgt agataaagat aatcctgaaa tggaaatata ataagttgtt gaatacttgt
    36601 taagatatta tatttactta atgatgttaa aaaggtatta aaaaagaaag gtcgtagtaa
    36661 atttgaaaaa aactataata aagaatgtaa atccaatgat tgaaaataag tttataggac
    36721 tttttgaaat agagtataaa aacaaattag atgaagataa ggtttggatg gtagcatcaa
    36781 gaaaaagtag tgagcaatta aaaaatattt atttgaagaa tgaagaagat agtgtagatg
    36841 cagttgtaat agttgggctt cataaaagta gtaaaaaatt aattttaata agacaattta
    36901 gagtacctat taatggatat atatacgagt taccagcagg tcttgttgat gaaggagagt
    36961 ctatagatat ttccgttgaa agagaactta gagaagaaac aggacttact cttttagaaa
    37021 ttaaaaaaaa taaaagtatt gataaagttt atttatctcc tggaatgagt gatgaatctg
    37081 ttgcctttgt ttattgtata tgtgatggtg atataacaga tgaattttta gaaccagatg
    37141 aagagattga agcgctttta atttcgcaag aagatgcaaa agagatttta caaagtaatc
    37201 ataagataga cacaaaagca tttttgattt tacaaatgtt tgtgagttta ggagctaaat
    37261 tatttgaata gatatataga aaagaggtta tatagaagag taaaataata aagtttattt
    37321 tacttgtgta aattataatc tcttttttta tacaataaag tataattttg tgtattttta
    37381 aataagtgat ttgactctta aattttcata atatgttata aaccaattta agatactaca
    37441 ataattaaaa ttccaaaatt atttaacata tttgcaatta aaatgagaaa atgtaaaggc
    37501 tgaatgattg aaaaatttga tgtaaaatag tagcttagtt taatactata gtttgtaggg
    37561 ttaaaaatta gaaattgtaa gggagataaa ttctatgaaa aaattagtta ttattttaat
    37621 tagtatcatt agtatggtta gcttgacagc ttgtaaaaaa agcaatgaaa gtaaatataa
    37681 taatttaaac aacaaagaat ccggacaaac aaccattgaa atggacttag ataaaaatta
    37741 tgatacttca gacccatttg ttaatgcacg tttgttttgt gtgtctaatg atatagatgt
    37801 tttagacaca gagatttctt ttaaaatgga tggagatagt ggtattgtgg aaataaagga
    37861 caataaaaca gatgaaactt tatggagtaa tacatggcat ggaagcgttg ataatgatac
    37921 attcacaatt tctctttcta atatacaaaa agagaaagag tatgctatat gctttactgg
    37981 aattaaaata aatcatgcag ttgtaaaggt tacttttgaa agcaatttag ttaaggagaa
    38041 agaaaaaccc tcaaaataaa ttccagcttt tctataactt gatattcagt acataaaagc
    38101 agttgatatt tatgattgac tgctttttct tatcaaaaag gataggttgt gcatttttac
    38161 ttgaaatagt ttatgcacat caaaagttgg tgagcatgat attggaaatg gtaagcatga
    38221 aatcttgata atacaaggaa ataaatttaa gttttcaatt aagtatattt ccataattta
    38281 gttaacaata agctaattat tatttatatt ttgatatata aataataatt gtagcaaatt
    38341 ttaaaaaatc atatattttt tgaactaaat aaaaatttat ttttattatt ttaaattaga
    38401 ggagattctt gaagatatat tatttttaaa aataagtata gtttaaaaat tcaaaaaaaa
    38461 gatggcaaaa tatgtcccaa gggacattga gtaaaataaa tgatttcagc ttatttacac
    38521 taaaaagtac atactaaatg aggggtaaaa taaagaaaaa tgatatacta attgtctttt
    38581 gataataaaa aaacatggaa tttgattatt taatcaagaa aaagtattga acaaatgatt
    38641 tatttagtgt acaataatca tgtaaaaaca tagtaatgga gggagacatt tcataatgaa
    38701 aattaaggta ggaatgaacg gatttggaag aataggaaga gctgtattaa gaatagctca
    38761 agaagaatta ggagataata tagaaattgt agccataaat gcaagagcaa caacagaatc
    38821 tttggcacat ttatttacat atgattcatg ctatggaact tttagaggtg aagtagaggc
    38881 aaaggatgaa gacactctag tagtaaactc taaagagata aaaatattaa gatataatga
    38941 cccagaagaa ttaccttgga aagaattggg ggttgatata gtaatagaat caacaggatt
    39001 atttacacaa agagaaaagg cagaaaaaca tattaaagca ggagcaaaaa aagtaattat
    39061 aacagctcca ggaaaaaatg aagatataac tatagttatc ggagttaatg agaaagaata
    39121 tgataatgaa aagcacaata taatttcaaa tgcatcttgt actacaaatt gtctagctcc
    39181 ttttgctaaa gtattggacg aaaaatttgg tatagtaaaa gggttaatga ctacagtaca
    39241 ttcatatact aacgatcaaa gaatattaga taaaagccat aaagatttga gaagagcaag
    39301 agcagcggct gaatctataa tacctaccac tacaggagca gcaaaagctg tgtcaagagt
    39361 actaccacaa ttagaaggaa aattaaatgg tttttcactt agagtaccaa caccaacagt
    39421 ttcacttgtt gatttagtgt gcgaacttaa agaaaacgtt acatctgagc aagttaactc
    39481 tgtgttaaaa gatgctgctg aaggagaatt aaaaggtgtt ttaggatatt gtgacaagcc
    39541 acttgtttct atagattata aaggagattc aagatcatct attatagacg ctctatccac
    39601 aatggttata gaagacaata tggttaaggt agtttcttgg tatgataatg aatggggata
    39661 ttcttcaaga actgttgact tagtaaaata tgtagctgaa aaattaaaat aatatatatt
    39721 tcttaaagca aattttaaaa aaatttttta aaaaaattat ataaaactaa aaaaaaagat
    39781 tatgaaaaat attgaaggaa aaattttttc ataatctttt ttctttcatg caaaaagata
    39841 caagttacaa cttatatttg catattaggt cgttagagaa gtatatatat atttagtaag
    39901 gaatcatact ttttataaaa tgaaagataa gatgtcgaaa acttgcataa atctgaaaaa
    39961 tttttattat taaaatactg atttccattt taaaaatgat aaaaaaatga caaataaagt
    40021 tgcaaatagt aatacttata agtataatta aaaaagagaa aaggttttgt taaactttat
    40081 aacaaaaaaa gaagggatga ttcaaaatgg agaagttaac agacaggaga caggttaagg
    40141 cagtaagcat ggaaaccttc attttcctaa tattactagt agttggtttt gggtatgttg
    40201 gtagtattat gggagcagga atgatgttca aagtaataat gagtacagca catgctttac
    40261 tattggagac agttttctta attatggcta tggcagttct tgcaggcgca ttaagcgcac
    40321 tactttcaga gtttggagta atagcgttaa ttaataaaat tttcgctgta tttatgaagc
    40381 ctttatatgg actacctggt gctagtatag caggagctat aacaacttat ttatcagata
    40441 atccagctat tatacctttt gctaaagata aaacattcac acagtatttt aaacaatacc
    40501 aagttccagc attatgtaac ttaggaacag cctttggtat gggacttatc ttaacaacat
    40561 ttatgatttc tcaaggtaca gaatatgtac ttccagcatt aattggtaat ttaggtgcaa
    40621 ttataggtag tgtcataagt gttagaataa tgttgacatt tactaagaag ttttataaat
    40681 acaatccgga agaagataaa gcaacaggaa cattagagaa aaaggatgaa tttagagaaa
    40741 tcagagaagg aaatgtattt caaagagctt tagatgcaat tcttgaaggt ggtaaaatgg
    40801 gggtagatat gggaatggct ataattcctg gagtcctagt agtatgtaca ttggtgatgt
    40861 tattaacttt tggaccatct acagatcctg ttacaggtca agaagtttat acaggagctg
    40921 catatgaagg aataaaatta cttccagtta ttggagataa acttggattt atattagaac
    40981 cattatttgg atttacatca ccagaggcaa tagcattccc tataacttca ttgggagctg
    41041 ttggcgcagc aatgtctcta gttccagagt ttataaagtc aggagctata actcctaatg
    41101 atatagcagt atttacagct atgggtatgt gttggagtgg atatttaagt acacatattg
    41161 gtatgatgga tgcattaaat gctagacagt tggcaggtaa agctattctt tctcatacaa
    41221 taggtggttt atgtgcagga gcagctgctc actttatatt tacactcgta ggataaatgt
    41281 aatcagctaa gataaaaaat aatatacaaa aaatggttgt tgatatgtaa taagaattta
    41341 catatcaaca accatttttt tctgactaaa gataatttat aatacataaa cattaagtgg
    41401 atattagagt taagttatat ttatgtaaaa agtaacaaaa atctgtagaa atttatgaca
    41461 aaatttagta cactaactat atgttcttat ttaaaattta aggttagaat taggaattac
    41521 ctagtagagt aagctttgat taataaaaga atcataaaat aaaaatatta gtcataaaga
    41581 ttataaataa cttacttaaa ataaagccat agaacttaca aaaattaaaa aagcatataa
    41641 atatggaggg gtacaatgga tgaattattt catataggaa ttgatgtagg ttctacaacg
    41701 gtgaaggttg tggtacttaa taatagtaat aatatagttc ataaagaata tagaagacat
    41761 tattctgatg taaaaaaatc agttaaagaa gtactaaatg gtatatatga aaaattggga
    41821 gatataaata ctaccattat aataactggt tcaggtggaa ttggaatatc aaaaaaatta
    41881 ggtgtaaaat ttgttcagga agttatatct agtacaaaat caatagagta ttttcatcct
    41941 gaaactgatg ttgtcataga acttgggggt gaagatgcaa agataacata tctaagtgga
    42001 ggaatagacc aaagaatgaa tggaatatgt gcaggtggca ctggagcatt catagaccag
    42061 atggcatcac ttttaaagac agatgccagt ggattaaatg aacttgctaa agggtataat
    42121 gttatatatc ccatagcttc aagatgtggt gtttttgcta aaacagatat tcaacctctt
    42181 ataaatgatg gagccaatca gactgatata gcaatgagta ttttcaatgc agttgtcgta
    42241 caaactgtga gtgtcctttc ttgtggaaga aaaatagaag gaaatgttgc gtttttgggt
    42301 ggtccattgt actttttatc tgaacttaga gaagcattta aaagagtatt agatttaaaa
    42361 gatgaaaaca taatcttccc agaaaatgca caactttata ttgctattgg agcaggatta
    42421 ttatcagtag atgaagataa tatacaacta aaatctctaa tagaaaaatt ggatagtata
    42481 aagaatattg aagatggaga agtaaattta ttagaacctt tatttaaaga taaaaatgag
    42541 tatgaagaat tttcaagaag acatgaaaaa gaaaaaataa attatgtgga cataaattca
    42601 ataaaaagca attgttattt aggaattgat gcaggctcaa ctacaactaa agcagcactt
    42661 attgatgagg atggaagact tgtatattct tattataata gcaatgaagg aaatccatta
    42721 aaaacaacca ttaaagttat aaatgaagta tatgatatat taccaaaaaa tattaaaata
    42781 ttgagttcaa ctgtaactgg atatggagaa ggtcttatta aaaaagctct taaaatcgat
    42841 aatggagaga tagagactat agcacattac aaagctgcta aatttttcaa caaagatgta
    42901 gattttatac ttgatattgg tggtcaagat atgaagtgcc taaaaataaa agatggagta
    42961 attgataata taatattaaa tgaggcttgt tcatcaggtt gtggctcatt tttagagaca
    43021 tttgcaagtt ctttatctat gacaatagaa gaatttgcgc atgaaggtat ttattctcaa
    43081 aatcctgtag atttaggttc aagatgtact gtatttatga attctagagt aaaacaagct
    43141 caaaaagaag gtgcaagtgt tggagatata tcagcagggc tatcttattc agtaataaaa
    43201 aatgctttat ttaaagtaat aaagattaga gatttaaatg agattggtaa taatattgtt
    43261 gtacaaggtg gaacctttta taatgatttg gtgcttagaa gctttgaaaa actaattgga
    43321 aaaaatgtaa ttagacctaa tatttcagga attatgggag cttttggttc agcacttata
    43381 gctaaagaaa aatatgaaag tggatacaaa acatccttgt tatctaagga agagttgaat
    43441 aatatcaagc tagaagcaag tgtaactaga tgtaaaggtt gttcaaatca ttgtcttctt
    43501 acaataaata aattttctga taatgaaatt tttatttcag gtaatagatg tgagaaaggt
    43561 gaagctattt atggtgataa aaaaatagga ttagaaaaga aaaaaccaat aaatttattt
    43621 aagtacaaat acaatagaat ttttagatac aaaccattgg aaaaagaaga agcaaaaaat
    43681 ggggaaatag ggataccaag agtattaaat atgtatgaag attatccatt ttggtttact
    43741 tttttcaatg ctatgggctt tagagttgta ctttcagata ggtcatcaaa acagttatat
    43801 gaaactggta taactagtat tgcatctgaa acagtttgtt atccaggaaa attagttcat
    43861 ggacatatag aaaatttaat tgcaaaaggt ataaaaaata tattttatcc aagtgtaaca
    43921 aatgaaaata aagaggattc aaatgcagat aattattata attgtcctgt tgtaatatca
    43981 tattctgaag taataaaaaa caatgtagag aatataagaa ataagaacat aaattatata
    44041 aatccattta taagtctaaa tgacaaggaa aaattgaaga aaagactcta tgatgaatta
    44101 tcaagacatt tttctgatat aaaaataact aaagaagaag ttaatcatgc agttgataaa
    44161 gctactgatg aacagaattt atttaaaatg gagatacaag cagcaggtga aagagcactt
    44221 aaagaaataa aagaaagaaa tatgaagggg attgtgttat gtggaagacc atatcatata
    44281 gacccagaaa taaatcatgg aatgccagag cttataaatt cattagatat ggcagtgctt
    44341 actgaagact ctatatgtca tttagccaat gtacaaagac cacttagagt tgtagaccaa
    44401 tgggtatatc attcaagatt gtataaagca gctagttttg taagagataa aaactatttg
    44461 gaacttgttc aacttaattc atttggctgt ggattagatg cagtaactac agaccaggtt
    44521 caagaaatac ttaatgaaaa atcgaagata tatactatta taaagattga cgaaggcaac
    44581 aatttaggag cagctaagat aaggatgagg tccttaaaag cagctatgtt tgagagagaa
    44641 agcaaaaata tagatgtaaa aaatataaag gtaaacagga tacagtatgc taaaaataat
    44701 cagataacga acaaacatac tatattagca ccacaaatgt ctccaataca tttccaattc
    44761 cttgaaaaag ctgttaactt aagtggttat aatatagaaa ttctaaaaga tactgatagt
    44821 agtgtaattg aagaagggtt aagatatgta aataatgatg catgttatcc agctataata
    44881 gttgtaggtc agctaataac agcattaaaa tctggcaaat atgatttaaa taacacttct
    44941 gttactataa ctcagactgg tggaggttgt agagctacaa attatattgg attcttaaga
    45001 aaggctatat acgatgctgg atttaaagat attcctgtca ttgcacttag tgtaaatgga
    45061 atagaagata gtggtataat ggataatata tcactaaaat taatcaatag actctttatg
    45121 tctgttgttt atggtgattt gctgatgaaa gtactatata gagtaagacc atatgaaaaa
    45181 gttactggaa gtgcaaatgc tctatatgaa aaatgggttg atatatgcaa atcttctctt
    45241 gttaaagcaa aaatttctgt atttactaga aatataaaag atatagtaaa agaatttgat
    45301 aatttagaga tactagatat caaaaaacca aaagtaggtt tggttggaga aatattggtg
    45361 aaattccatc caattgcaaa taataattta gtagatatat tagagcgaga aggtgctgaa
    45421 gcagtcgtgc cagaccttac aaatttcttc ttaagttgtg cctttaatac tatatacaaa
    45481 cacactcatt tagaaggaag tagaaagagt agaatgatag gagaagcatt tatatacata
    45541 actggaatat atcaaagagt atataaaaaa gcacttgata agagtgagag attttatgct
    45601 ccagccaata taaaaaatgt tgctaaaagt acagaaccag tagtgtcatt aggtaatcaa
    45661 actggagaag gttggctttt aacaggtgag atggttgaat tattaaatga aggtgtagaa
    45721 aacattattt gtatgcaacc ttttggatgt ttacctaatc acataattgg taagggttct
    45781 ataaaagaat taaagaggtt gtataaaaat gcaaatataa tacctattga ttatgaccct
    45841 tctgcaagtg aggtaaatca acttaacagg ataaaactta tgctttctaa ggcttttaaa
    45901 aatatataag atttatattt tgatatgtta aaagttaatg aatttaaaaa tatactctat
    45961 attaagtatc tataaaaaag agttatccta ttttttaggg taactcttta ttagttaaaa
    46021 aattatatgt atactgatat taaagggggg aagtaatatg agtttagtat atgatgttta
    46081 taaatcaaaa ttaggaaatt tgtacatatt aagtgaagat ggttttatag ttagtatata
    46141 cataggagat gaaaaattta ataatttaaa aaatgaatgt attaaaaaag gaattgatat
    46201 aaagagaagt aaggaagatt taaaggaagc aataagccag atagatgatt actttaatgg
    46261 aaaaagaaaa gtctttaatt taaatattcg tattgaaggg actgaatttc aaaaatcagt
    46321 ttatagggaa atgttaaaaa taccttatgg agaaacattg tgttattcag atatagcaag
    46381 aaatataaac aatccaaaat cagtaagagc aataggacaa gcaagtaaaa gtaataaaat
    46441 tccaataata ataccttgtc atagagtagt tggcaaaaaa gatattggtg gatatatggg
    46501 gaatcattct gatttgaaag agatactatt aaatttagaa agacagagtt aacttagaaa
    46561 gaaatgtttt tattgttaat atttgatata ataatagtaa aaagaagtat tttttacaaa
    46621 aacaatgaag aatttatttt aatttataaa aatgaggggg aattttaatg tgtgataaag
    46681 ttatagcatc aaaatatcat gagaagggat ttaattgtgc agaatcagtt ataaaagcat
    46741 ataatgagga atttaataca gatataccag tttgtttagg aagtggactg ggttcagggt
    46801 gtggggttgc tagtttatgt ggtgcagtaa atgcatcaaa tataataatt ggatatgtaa
    46861 aaggcagaaa ccacgaagat gaaagtacta aagctaaagt atatgctaaa gatttaacta
    46921 caacagtaag aaaagagtat ggttcagagt tatgtataga cctaaaaaaa gatttagttg
    46981 cttgtagaga aataatggat ttcgcatatg atagtctaaa ggaaacttta aaaaaagaat
    47041 tataagaaaa taagctatca agtataaatt atttgatagc ttatttttat agtcgatact
    47101 aaataaatca aatatatttt agaattatta tttgtaaata aacagcaaat aattttagga
    47161 tttttattta atatagtttt tatttatatt tatttttgaa tatgttatac tttgtttaaa
    47221 taaatatttg taatgaggga gatgagaaga ttgaagaatc tcaatttact tattaataaa
    47281 ttatattcaa aaaatcataa tgaagcatat aaaacttttt tattcttaga aaatgaaagt
    47341 ttaaaatcaa atattacata ttgttttttt gactcattcc tagaaatgat tgataacgaa
    47401 aactcatata ttcgaacaag gggattgtta ttaatctctg caaatgctca gtgggatatt
    47461 gataataaaa ttgaaataaa tatagatagt atactaagtc atatcgtaga taaaaaacct
    47521 tctgtatcaa gaatgtttat taaatccata cctaatatta caaagtataa agaaaatctt
    47581 attaatcgca taaaaatgga attaagtaat gcagatataa gtatttataa taataatatg
    47641 aaaccattag tagaaaagga tataaacgat acattatcta atattagtta aatattaata
    47701 tagaaattga atattatcta gttacaa
//
LOCUS       DAMJWS010000020        46812 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMJWS010000020 DAMJWS010000000
VERSION     DAMJWS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.020"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(180..959)
                     /gene="hisJ"
                     /locus_tag="PC399_001226"
     CDS             complement(180..959)
                     /gene="hisJ"
                     /locus_tag="PC399_001226"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF2592099.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLILGSDSHCAEEINKYDIV"
     gene            complement(1505..1855)
                     /locus_tag="PC399_001227"
     CDS             complement(1505..1855)
                     /locus_tag="PC399_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF2592100.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(1871..2287)
                     /locus_tag="PC399_001228"
     CDS             complement(1871..2287)
                     /locus_tag="PC399_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF2592101.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(2306..2497)
                     /locus_tag="PC399_001229"
     CDS             complement(2306..2497)
                     /locus_tag="PC399_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF2592102.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(2648..3463)
                     /locus_tag="PC399_001230"
     CDS             complement(2648..3463)
                     /locus_tag="PC399_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF2592103.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(3510..4448)
                     /locus_tag="PC399_001231"
     CDS             complement(3510..4448)
                     /locus_tag="PC399_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2592104.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(4445..5317)
                     /locus_tag="PC399_001232"
     CDS             complement(4445..5317)
                     /locus_tag="PC399_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2592105.1"
                     /translation="MNKNLSEKMEIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(5342..7270)
                     /locus_tag="PC399_001233"
     CDS             complement(5342..7270)
                     /locus_tag="PC399_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF2592106.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(7382..7759)
                     /gene="gcvH"
                     /locus_tag="PC399_001234"
     CDS             complement(7382..7759)
                     /gene="gcvH"
                     /locus_tag="PC399_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF2592107.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(7846..9972)
                     /gene="cdhC"
                     /locus_tag="PC399_001235"
     CDS             complement(7846..9972)
                     /gene="cdhC"
                     /locus_tag="PC399_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF2592108.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10029..10835)
                     /locus_tag="PC399_001236"
     CDS             complement(10029..10835)
                     /locus_tag="PC399_001236"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2592109.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(10885..12252)
                     /locus_tag="PC399_001237"
     CDS             complement(10885..12252)
                     /locus_tag="PC399_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF2592110.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(12283..13227)
                     /locus_tag="PC399_001238"
     CDS             complement(12283..13227)
                     /locus_tag="PC399_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF2592111.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(13247..14017)
                     /locus_tag="PC399_001239"
     CDS             complement(13247..14017)
                     /locus_tag="PC399_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2592112.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14073..15458)
                     /gene="lpdA"
                     /locus_tag="PC399_001240"
     CDS             complement(14073..15458)
                     /gene="lpdA"
                     /locus_tag="PC399_001240"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2592113.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(15502..16383)
                     /locus_tag="PC399_001241"
     CDS             complement(15502..16383)
                     /locus_tag="PC399_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF2592114.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(16419..17093)
                     /locus_tag="PC399_001242"
     CDS             complement(16419..17093)
                     /locus_tag="PC399_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF2592115.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(17130..18002)
                     /locus_tag="PC399_001243"
     CDS             complement(17130..18002)
                     /locus_tag="PC399_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF2592116.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18094..18726)
                     /locus_tag="PC399_001244"
     CDS             complement(18094..18726)
                     /locus_tag="PC399_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF2592117.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(18831..20507)
                     /locus_tag="PC399_001245"
     CDS             complement(18831..20507)
                     /locus_tag="PC399_001245"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF2592118.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(20756..21535)
                     /locus_tag="PC399_001246"
     CDS             complement(20756..21535)
                     /locus_tag="PC399_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2592119.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(21845..23764)
                     /gene="cooS"
                     /locus_tag="PC399_001247"
     CDS             complement(21845..23764)
                     /gene="cooS"
                     /locus_tag="PC399_001247"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2592120.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(24371..25282)
                     /locus_tag="PC399_001248"
     CDS             complement(24371..25282)
                     /locus_tag="PC399_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2592121.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(25398..25976)
                     /locus_tag="PC399_001249"
     CDS             complement(25398..25976)
                     /locus_tag="PC399_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2592122.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRAIDEIPE"
     gene            26202..27125
                     /locus_tag="PC399_001250"
     CDS             26202..27125
                     /locus_tag="PC399_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2592123.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(27297..28439)
                     /locus_tag="PC399_001251"
     CDS             complement(27297..28439)
                     /locus_tag="PC399_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2592124.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(28457..30211)
                     /locus_tag="PC399_001252"
     CDS             complement(28457..30211)
                     /locus_tag="PC399_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF2592125.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            30347..30553
                     /locus_tag="PC399_001253"
     CDS             30347..30553
                     /locus_tag="PC399_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592126.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(30558..31481)
                     /locus_tag="PC399_001254"
     CDS             complement(30558..31481)
                     /locus_tag="PC399_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592127.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(31735..33435)
                     /locus_tag="PC399_001255"
     CDS             complement(31735..33435)
                     /locus_tag="PC399_001255"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF2592128.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(33845..34258)
                     /locus_tag="PC399_001256"
     CDS             complement(33845..34258)
                     /locus_tag="PC399_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2592129.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(34324..36702)
                     /locus_tag="PC399_001257"
     CDS             complement(34324..36702)
                     /locus_tag="PC399_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2592130.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            37021..38472
                     /locus_tag="PC399_001258"
     CDS             37021..38472
                     /locus_tag="PC399_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF2592131.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(38676..41189)
                     /locus_tag="PC399_001259"
     CDS             complement(38676..41189)
                     /locus_tag="PC399_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2592132.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTSQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKALDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(41412..42233)
                     /locus_tag="PC399_001260"
     CDS             complement(41412..42233)
                     /locus_tag="PC399_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2592133.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(42751..43227)
                     /locus_tag="PC399_001261"
     CDS             complement(42751..43227)
                     /locus_tag="PC399_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF2592134.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(43262..43840)
                     /locus_tag="PC399_001262"
     CDS             complement(43262..43840)
                     /locus_tag="PC399_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2592135.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGNEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(43944..46544)
                     /locus_tag="PC399_001263"
     CDS             complement(43944..46544)
                     /locus_tag="PC399_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2592136.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHSNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNVFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        14177 a         9044 c         5245 g        18346 t
ORIGIN      
        1 agtcctatta ttctattgaa ttttattatt attttattta gattctattg ttattttact
       61 tagaacttat tattattttg tttaagttct attactattt tcaatcatat aaatttacaa
      121 aagactgtac ataaatttat tttatacaaa cttatctaca gtcttttgtg tgaatatatc
      181 taaacaatat cgtatttatt tatttcctct gcacagtgag aatcagaacc taaaattaat
      241 ggaatatcgt atttttttac caactctaat aagtatccat ctatataagg ctctttgcag
      301 tactcttttc tatttccaga aacattataa tcaagttcat aacctttttc tttaattaac
      361 tttattaact cttcaagaat tgtatttcct ttataatcgt atggaaatac ctgattaaac
      421 tttctaacta aatttaaatg acctattctt tttggtttat gtatacctaa atctgaattt
      481 acagctttta tcaatgtttc ataatactta ttataaacag cttcaatact tcctaatttg
      541 tcgattaaat tttgaaactc ctctacactg taatctacac aataataatc atcatcaatc
      601 ttaattatat gtactgataa aagagaatcc tctaattcct ttccatattt atttaaattc
      661 tcctttatcc cttcttcgta tccttctata aaatccactt ccgacccaac atttatcttg
      721 attttaccct catacttttc tttcaacttc ttgacatcat taaaatactt tggtaaatct
      781 tcaatactca tacaactatc attttcagga gcagggtctc taaatccatt tgcatacgga
      841 aaatgttctg taaatgtcat ttctgttatt ccaacatcca ttgctctttg tacatatttt
      901 tcaaaatcat ccttacttcc atgaggacaa taaggtgaat gtatatgacc atctttcata
      961 aataattcac tcctttttta aagaaaaaat ctaatataaa tgcataaatc ttttaacttc
     1021 aaaataatat ttttaatcat gctaattttt attatatcta atattagagt aattttaaac
     1081 tgccttttaa gtatcttatc attagtacta ttttcaactt aacattaata ttatttttta
     1141 acttaacatt aatactatct ttaaatagta atactcaata tataattact taacataaaa
     1201 taactcaaat tgcttgatga ggcttttgca gtatttaaag aatatataat tatttaacat
     1261 taaataatcc aatcttgtaa taaaatttac tcattaaagt atattacaga aaatacctca
     1321 aaataaactt tttagttcat gatatactct tttttgcatg aaattagata tatttttatc
     1381 atttcaacaa tgaaagggag gcttatatcc atctttagac aaccccttag ataaaatttt
     1441 taataattaa acatcacaaa atataaaaat cgcttatatc aaacgtataa gcgattaatt
     1501 atatttacat atgtttttta gcctctaaca acattttatt agcttcttcc atatactcta
     1561 tagccttttc aatactcttt gctgtttctt cttttccaat agctctagcc ttttcaaccc
     1621 actctctaaa tccttcttca tgagtttcat tgtgatttat ccaatgtact aaaagtattt
     1681 ttaaagtttt ttcatcctta gactctgatg attcctcatg actatgttcg tggtcgtggt
     1741 cgtgagcatg gtcatgtgga tgttcatgac atatatctcc atgagtatgt tcgtggcagt
     1801 ggtcatggtc atgatgtcca tgatgatatc ttacaatctc tttatcaaaa atcattagtt
     1861 attccccctg ttattttata ttaaactctt tttttataag gtttacagaa tcttttacaa
     1921 gtagtgctaa tggtttacct tctaatccaa ctaactccac attttctggc ataataggta
     1981 aaagtatctt aagtgcctca ctgtctgcaa tagcttcact tatattacaa gtaacttctc
     2041 ccatcataga attaggtatt attacagaaa taggtgcaac tattatattt gcttttttag
     2101 ctgatactac tatagcattt tcccctgtag ctcctttatt agcatgagct ttcatcatag
     2161 aactagttgc tatagaatta gtccctagtg cataaatttc tacatatgta ggtaactctt
     2221 ctctcaaaga agaaactatc tgggctccaa tacctccacc cattccatct atcactgcta
     2281 ttatcatatg aaattcccct attaattaat tttcttgtat tattatctta tggtccatca
     2341 atcttatttc tttaattatc ccatctatta tcttttgctc tcctaataaa tccgttaaat
     2401 aaatctttcc atcacaaggg tctatattaa ccacattttc cataaccttt tctaactcat
     2461 tattactttt acaaatgaaa gctgatgatt cacacatata aatctcctcc taatatgatt
     2521 tttattaaaa atcaaaaaaa gattccctta ggtatttcaa tacaccaaag aaaccttcat
     2581 gatttcagtt tttgtatatt attatacctt aagtatatca ctttaattta tgtaaagtca
     2641 attaaagtta acgcttttta ttatactctt cattcttgta tttttcatat agttctttaa
     2701 ctaattgctt ctcataatca gtcaaatcgc cctccacaaa ctcaacatta aattgttcag
     2761 ctaatccctt tactatgttt ttctgtagaa tatctatgtc tatttttctg ccaatttcat
     2821 tttctattcc acttgctttt ttagctgtaa atttcatagc tctttctttc aattctggtg
     2881 tcttagtttt tattaattta aacaattttt ctacatcaaa atccagtatt attgaaccat
     2941 gttgtaataa aacaccatct tttctgcttt gagcacttcc aattaccttc ttattattaa
     3001 tagtaacttc atatgatgca tgtgcattaa aacatgcagc tgataaattt tccctactta
     3061 ttctctcgcc tctatttaaa ttatctgttt ctataccact tagattcaac cctttaacta
     3121 gaccttcact tatatatcta taagataggt tgatgctctt atcgattaaa ggattatctt
     3181 ctcctataat tatactataa gttagctcat tatcatgtaa tacagctctt ccacctgtaa
     3241 ttcttctagt atagtctata ttcatacatc tacacttatc taaatctatc tcatcttcca
     3301 atttctgaaa atatcctata ctcaaacaag ctggttccca tgtataaaat ctaagagttg
     3361 gcttattatg tcccttctta tatgcagtaa atatagcttc gtcaattgcc atattcattg
     3421 ctccttcata tgatttatta tgtataactc tccactgatt catgattatt acgcccccat
     3481 ttttagtata aatatatcct agaaatcctc tatagattta tttctcttat tgctaaatct
     3541 atctcacttt gatttaaagg tcttgaatag ttgtatatag tacttcctgg tctttcatta
     3601 tagtatggtc tattacaatc cttacaacct cttatttcaa aaggatagcc tttgtttatg
     3661 tcttttaaaa tatcattgtc aagctttata ctatttaagt atccattttt aaacttaaaa
     3721 tactcttttg gataaccttt atttatcata taagacatta actgtaccct tctataactt
     3781 tctatgcttg gttgatttat cttttccatt ttagttcctc taactggagt aaatgcaaat
     3841 aaactaatcg ttacatcatt ttcctttaaa taagtatata aattgtatat atcttcatga
     3901 ctttcaccca taccaactat tatatgtgta cttattttat tgggatatga ttttgacatt
     3961 tcagttataa attttaactt ttcatcatag ttatttcctt ttattttttc atatagttct
     4021 ttatttgctg catctattgc tattcctatt ttatcaactc ctactgaaaa aaacttcttt
     4081 atctgttcat cattttctaa ttttgcagat agtgaaattg gcatgtctat cttaccactt
     4141 atatagttta taaagtctaa aacagagtta tgtgcttctt cacttgccat tgattgaata
     4201 catattctct ttatattttt tcctttataa gcttttaaag catctaatat ttcttctttt
     4261 gaaaactctg gccaaattac tcttgataat ttatcttttc tagtactact ttctattgat
     4321 tgagaacaaa aactgcattt atttatgcat ttttctccaa tcattatata tgctgtggta
     4381 ggaggtatat cgcttttttt gtttaatatc ccaagttcta ttgctgtccc aactgatagt
     4441 ctaatcataa aacgcaacac tccttacact catttattgt catattcatt tctatagcct
     4501 tattctttgc agaccttgat ggtaatacta ttctattgac tccacacatt atagataatt
     4561 ggtctagctc ttttctatac actcctcttg gtctcataca acctagatta atttcagtat
     4621 ctggtaaatt aattctagct tcacataaaa tatctgctac cccttctaac tcaggaggtt
     4681 ctacattttc atactcagtc ccttttgttg gaataaggac tataaatgta agcttattag
     4741 gaggattttt ttgtaggtat tctataattt catattcacc ctttatttgt cctcctttta
     4801 aaccaataca tatatgtggt gctacttctg tatgttcttg tattgtatta tagacttcaa
     4861 tataatcttc tttactcttt ttcatcttat aaacttctct tattgtctca tcatcaaaca
     4921 ctaaatcaaa agaaactata tctaaatact tacacaactc tacaatgcta tctttatcca
     4981 taagacctaa atgagcattc aatctatatc cttgctcttt taagttttta atggtattaa
     5041 tatgagtatt tatagggaca tctccctcat aactacaacc gccactcaat agaaagctag
     5101 ttatattcct tgattgtact ttttcctcat attcttctat tggcaccata ttgtttaagt
     5161 agtgaccgtt gcaatgggca catttcaaac tacattgatt gcttgtagta ctaagagcta
     5221 gtgtctggtt aggataagca aactctattt tatttccaaa attctttaat ttaatatcaa
     5281 atgctatttc cattttttca gatagattct tattcatagt ttattccttc ttaagtacat
     5341 tttactcttc tagtaatttc tttgctgttg ggaactgctc tatattcgta tgtggtaaga
     5401 aacatgcaga aataaattca tccatataac ttggatatac actaagttca acataagtta
     5461 tttgattacc tatttcttca agtttatttt ttgcatcttt acttataaga gctaagtaag
     5521 aaccaacaag tgaactattc cctatataag taaatttttc tctatcaata tcaggaagta
     5581 accctataat tattgagttt tctatgtcta ggttgtttcc tataccacct gctatataaa
     5641 ccctatctaa tatactaaaa tccataccta aactatctac aagagtgtat gcgcctgaat
     5701 atatagctcc tttagctctg atgaaattat ctatatcaac ttcattgact actatatcat
     5761 tttctaaatc aaattcttct ttaaatgcta atacatactc tcctatctca tgctcattaa
     5821 atctaactct cttattatct aaatccctgt atattttacc tcttctatct attacccctt
     5881 tagtaatcat ttgacagatt aaatcaatta taccagaacc acatatacca actggtgcac
     5941 attcatcaat aatctttaat gttggctcta aagtttcttt atcaataatt actttctcta
     6001 ttgctcctgc tgatgctctc attccacagc ttatccctcc accttcaaaa gcaggtcctg
     6061 ctgaacatgc acagctcatc atataatctt tatttccaaa tacaatctct ccattagttc
     6121 ctaaatctat aaataaaaca ttttcttcac ttgaccatat ccctgctgat aatacacctg
     6181 ctgttatatc tcctcctaca taacttgcaa cagatggagc taagtacaca tatgcactgt
     6241 catttacaaa aagaccaaca ttttcaccca taagttttgg agattttaaa aatggtggta
     6301 tgtaaggttc ttgtcttaag aagtctgtat acacacccaa gaataaactt gtcatagttg
     6361 tattcccagc agccactaat gtaactacat cttccttatc tattccattt ctttcatata
     6421 ttgtttttaa tagtggattt atagtttcct caactatagc tttatgaagt gtctctaatc
     6481 catttttctt agttgaatat ataattctat ttataacatc tgctccatat tttatctgtg
     6541 catttccaga agatgcttta tctactacct cttttgagta taggtctact aaacaaacaa
     6601 ctactgaagt tgtaccaata tctattgcaa ctccgtataa ggaattttct ttatttcctt
     6661 gttctatatt tatgattgta agtttctttt gtttttgaac atatgttatt gtaaccttaa
     6721 aatcactctt tctaaataca gttggcattt ttctaagtat atccaatcta aaatctattt
     6781 cattatatcc taggttattt ctaacatatc tttccaatct atctacatca cttatattat
     6841 catctaaatt tggttcttcc atttctatgt atttcttttt tatatttgtt ttaaattgaa
     6901 ggttatgttc ttcaattatt tttttagctc tttcaaaaat ttccctatcc tcttttttat
     6961 tactaccttc tatcttcata ccatgcatag aagatgatac tttagaagga acttcaattt
     7021 ctatatctga tattaccttt gtacaacaag ctagtatata accttgctcc cactcatcat
     7081 ctgttatatg tcttgttttt tctgtatcaa cttttccatt caataatttt actttacact
     7141 tcccacaaga cacgtttcca ttacaaggag catctatgaa tatatctgca tttcttgcaa
     7201 cctctagtaa tatatccccc tcattacaat aaacctcttt attgtttggt gtaaaactta
     7261 cttttatcat gttatccctc ccaatttgtt ctatatatga taaaaggaaa gtatagctaa
     7321 atttgcgtgc actcaaattt tactatactt tccccaatta taataatatc ttaatcttta
     7381 attattctaa tccagcctcg tattctttat cacttaataa cgtatcaagt tcagccacat
     7441 cagctagttt tacttttaat atccagtttt catatgggtc ttcatttata cattctggct
     7501 catcttctaa tttttcatta acttccaata cctcaccatt aacaggagat attaaatcag
     7561 aagctacttt agaagactca actactccaa aatctactcc cttagttaca gtatcttcta
     7621 cttctggtgt ttcaacaaac aatatttctc ctaattgatc ttgagcatag tctgttatac
     7681 ctatatatac aacatctcca tcaattactt ttacccactc atgatcttta gagtatttta
     7741 attctggtaa taatttcatt tcgatagacc cccaatatta tattattgat taaattactt
     7801 aaatcatatt aatattatat catattattt taaattttcc atcaattaca ttacactttc
     7861 catagctaat gctggatgac ctttttcttc taagaaagct aatactcctt cagaatcgtc
     7921 agctatagtt tcatcacaaa ccatatcaca gaagttttct attccagtca tttctttaac
     7981 tgtagcattt aatttatcag ctacataatc ttttaattct tttggcatcc aaactattct
     8041 ttctggtcca ccttctgcat aagcgaattt tttagaagat atgaaatgtc ttccgtgtcc
     8101 cataaatcct ggagtttgaa ctcctccacc tgtcatagaa gcaagttctc cgaaagtcat
     8161 accaactgga gtaacacttg caaattctct gttaactaca acaaatccat ttgcttctgg
     8221 cattatacca cagatacatt cgaagcatcc acaagaagtc attgggtctt ctagtataga
     8281 gtataatgta acagattcaa ctgcaccttg agatatttgg tttacagttt catttactga
     8341 attccataca cctgttctat catctaagca ctcgcctttt tctattggtt gacaaggacc
     8401 tgttgggtct aattctttag tagccttagc atctaaccag ctaactgctc cacaaagacc
     8461 aagtctttca ggagtaacaa cacaaacgtg agctggtgca aatgattgac ataagttaca
     8521 agaatagaaa gtatcaacac tttcatcaac taatgaagct aatctttcat ctctagcatt
     8581 gtacttagct attgcttctc cttttaattc agaaactttt tcagcatctg taattatagt
     8641 tatttcacac ttatcaacta ctgattcaaa ttcatctaac atcttagcat ataaaacttc
     8701 tcctatatgc tcaagtctaa atcctttatc aaatgcatct ttagagattc ttacccaagc
     8761 catatctctt tgtccaacgt gcattacacc ttctatatag tttaagaagt agtggaaacg
     8821 tctttcaaga actggctcga aatcttcttg catattttta ccagctattt taactattac
     8881 agcaagtggt aatcttaata caccatctgc atcaacttca tctatatttg gtcctattat
     8941 ttctatctta tggtcttcaa cttctgttgc ttctttctta acaacaagct cccaagcttc
     9001 agttctgttt ccaccaaact cagcaaacat atcgctcttt ctaattctct caccttcaaa
     9061 tgctgctgca aatgaaactg ggataggtat ttcagtaact tttattttta ttcctctagc
     9121 ttctaatgaa gtagctacta tcttatcgta atctttttga gttaataggt tcattggaac
     9181 ttcaagaact gtttggtcag ttataactgg gaatcctaaa gctattgctc cagcacctgc
     9241 agatacaact aattcactta atggtccaaa tgcattaaca aatgcaggaa ctctattagc
     9301 tgtgtactct aataatccgt ttaaatcacc aggagttaat ccaccgaata ttaatgctgc
     9361 tcttactgca acagatacaa cgtgtataac agaagttaca tcatatccta gaggtataac
     9421 tcttagctca agacccattt ttactccagc ttctatagct tggtctataa ctttaccaac
     9481 taagaaagtt agtaaacctt tagattggta atcttttata acttttgctg ctgattctgc
     9541 atctggacac tcaccaagaa caactgcaac accaggtata tctcctgtaa ctaatggtac
     9601 accaagtgat ctgattatag ggtcagttat atgacctgca catggctcac tatatggagc
     9661 atccatagtt gaatatttta atgcttcaat tacttctgct gctaaagctg tagctaaacc
     9721 agcattaaaa gcatgttcta gtaaatgagt tctatttatt aaagatttta ctacttctaa
     9781 agcaccttct aagtccccta aagtgttcat cttttgtcct gttgcagcat atatacaagg
     9841 taatgaatat gctgtatcag ggaaagcaac tttatgctct tttccattct tttctatagc
     9901 ttcagctaac atagcttgag ccgcacctaa agcttgttct gaccctgtaa agattatatt
     9961 atatagattc attctatttc cccctttaat atttacacct attttttatt aactataccc
    10021 taaaaaccct agaaagcaaa tccaccttca tttaactcta agaaagagtc tgcatataaa
    10081 gatgccccgt tgattatgtc acaagatttt acagttttca ttaattctgg gtcacatgga
    10141 ttcattatag ctgaactaaa tccattagcc attgccattg ctaagaatgc actatctaaa
    10201 acaggtctaa catgcttagg acatccatta gatacgttag ataatcctcc tgtagttaaa
    10261 agtcccatct cagtcatcat ttttatagct tctaaaactt ctacttgttt ttcttgcata
    10321 ccttttataa ctaagcataa tgggtcaaat agtatatcag ttggctccat tcctaacatt
    10381 aagccttttt ctaatatttc ttggcagtaa gccatacgct catcattatc tcttgggata
    10441 ccttcttttg cacataaacc tataaccatt gcttcgtatt cagctgctat atctattaaa
    10501 tcaaatcttg aaccagcatc agcagagttt attattggtt ttgcattagt tctatcataa
    10561 actttaagtc cagcttcgat agctttttta ttagctgtat ctaatgctat aggaacattg
    10621 ttgaactcag attgaagtaa tttgattccc catgtcatta tttcttcgcc atctctttcg
    10681 gcaggtccta tattaaaatc tatatagtgt gctcctgctt ctaattgctc ttttgctctt
    10741 tttaagattg gagctgggtc tctttctgct aatgcttttc ttattgaagg tgatatacaa
    10801 tgtattctct cacctataat cataaatttt tccatttata atgtccccct ttaaatttaa
    10861 tattaatagt tagttaatat tttattagtt tgtttggcat gctttttctt ggtatccttt
    10921 taagaactta ggtaattcca tagattcctc tggtcctata actacattcc atcctggtaa
    10981 gttatcttct atatcacctt taagtactgc aacttttcct ggaatgataa ggtctttaca
    11041 attagttact tcttctacct tactttcttt aatgaaagca gagattgagt ttccaccaaa
    11101 tttaccagca gcccaagatg taagaactga atatccacct gcatctggta ttactaacca
    11161 tactggaact tttgatcttt ctatatcacc agcaactatg aagtaagtta atgcaaagtc
    11221 aacagtaact aatactggag aattttcatc tgggttgttg attggataaa tctttggttc
    11281 aactctcatt ggtctttgtg ggtcagtgta tatgttttgt cttagtgcaa ataatggtaa
    11341 tgccttagca taactaatat catctattac tattatagaa ccgtatttta tagtgaatat
    11401 tgatgataaa gcaacttcca tcatagggtt tgaattagat aatctatttg caaatactat
    11461 tgaaggatat ccaaatgttc tatcttgctc ttttaaagct gttcttctaa cttgtatagc
    11521 attagtgtaa gtatctttta tgttttctcc tgttacatct aatactaatt ctttatatcc
    11581 agcagcttga attaattcaa ctgtttcata taattcttct aagctaccag cttttacacc
    11641 taatggtaaa cttgctcctt taactacttc tatcatatct ttgtagttct ctttagttgc
    11701 tccatatacc attggtctag catcttttaa tacttcaaca gcttctttaa ctacttgtgc
    11761 atcatcacaa gctaaaatat aagctacttc taatccactg ccttttattt tatctattaa
    11821 ttttaaatac gcatctttat ctccacaata ctctaaaaca gccatttcaa ctttcatttg
    11881 ttcaccaatt cttacatagt caactttttt catgttagct attttagcat ctactgcttc
    11941 atcactcata caagtacaga atgaaactgc atatctattt ctacttacta atgttttttc
    12001 atgtctaaat aatactgttt ctccacctag ttcatactca gatgcaccag caccaacttt
    12061 taaagctttc attaaaggtg cagtagcctc tgataatttt gctatagcgt catcagacat
    12121 atgtgggcat ttacctactt ctacagctcc tgaagcaacc ttcatagaga aagccataca
    12181 agttgggaat ccacaatcct tacagttttt ctttggtgtt aatttaaata tatctaaagc
    12241 ttttaatgcc attatttttt ccccctaacc ttaacaattt aattaagcta atgcgttaac
    12301 caattctttt atagtttcta ttgattttgg atgacgaagt ataactgcgt ttgaaccacc
    12361 tactaaaaca cttgctgcag ttgaaacttc cattgctata gttctttctt ctggacaacc
    12421 ccaatctggc tcatcttcta ttggagctat agcttcttta acatgaccta cttcaaaagc
    12481 tactggtgtt atgataggca tttgtaatgt cttatcattt tgaccaaatg ctgcaagtct
    12541 aattctatcc atagtagatg caacgtactc atatccataa ccaactgctg aacatccaac
    12601 attcataact atattttcac ctttaacgcc tagttgagtt aacaatacgt ttaattgttt
    12661 agctaggttt atatcaacag aagattcagc tcctacttta tgtgcataag ccataccagc
    12721 tgatgctcct actcctttgt agttatcttc tactgctgac ataaataagc agttatgtcc
    12781 atccaatgtt tgagctaatt tttcaaataa tttaccatct ttttcatgat ttcctgaacc
    12841 agctacaact aaaggtaatt ttattgcttc aattactgcc ttagctacat cagcacattc
    12901 atcaactgac ttatctaatc catttgggtc agaaccatca aattttaaac aaataaaatc
    12961 tgcttgtgta ttagcttcaa catatttagc ccactctact ggacaattag ctacatcttt
    13021 gtataattcc ttatatgaat cagtccagct ttcaggataa acatctgata tttgtatccc
    13081 tatttttgga gaattaccta cttctccatc aaaactataa aaaggtaata cattttcccc
    13141 acctaattta attgcttttt cccctattcc tacttcaact tctgatattt ttccagaata
    13201 tttttgagta gacattttaa atgccatatt tttttccccc ttaagcctat tttaattcta
    13261 atttagcaaa aatttctttc atagccactt tagattttga atcttcaggt aaattaacta
    13321 aaggaatacc tgtagaatca tattcatata tcaattcatc cataggtaca acaccgatta
    13381 gctctaggtt atgcttttca atttcttctt taacaccatc attcataacc ccatttggaa
    13441 ctttatttac aatcaaaagt attcttccaa cacttaactt tagttcctca gctaaatctc
    13501 ttattctagc aacagcttgt atgctacgtc ttgagcaatc actaaccaat aaaagtgtat
    13561 caacatgtct agtgactttt ctacttagat gttccatacc agcttcgttg tccatgacta
    13621 agtatttgta atgaccagat attttgttta cttgttctct aagtatccca tttacaaaac
    13681 agtaacatcc ttcaccttct gacctaccca ttactaataa gtcatatcct tcgccttctt
    13741 cgattataga atttaatcta aattgaagat attgtgcctt tgtcatacca ccagggaatg
    13801 cattgcctaa cttttctctt tgatttactt cttctcttat ttctcctatt gttgcttcaa
    13861 cttcgatacc taatacttca tttatgttag cattggcatc agcatctaca actaatactg
    13921 gtcccttttt gtccttaacc aaataatcaa tcaaaagccc tgtgagactt gttttaccag
    13981 ttccaccttt tcctgcaaca gctatattgt atcccatttt aagacttccc ccttaaatct
    14041 agccacagag ccttatgtac tctgtctgtc tattataatt taggtacaga atgaacacat
    14101 tctccatgta catcatgaag tgcttccatt agcccttcag ataaacttgg atgtggatgt
    14161 attgcatccc ctacttgttc tactgttaat cctagatgaa cagcaagtga caactctgtc
    14221 aataaatctg tagcatgagg tcctactact gctgcaccta ttatcttatc agtttctttg
    14281 tcagctataa cttttacaaa tccttggaaa tgtcctatag cttgtgcttt tccaagacct
    14341 ctaaaatcaa attgacctac attgtattct acaccttcag cttcaagttg cttttcagtc
    14401 ttaccaacac cagcaacttc aggttcagta taaacacatc ttggaattgc tctataatct
    14461 actaccttag tcttacctaa agcattttca actgctacga taccttcttt agaagcaaca
    14521 tgtgctagga aaggagtatc tattatatcg cctattgcgt atataccttc tacattagtt
    14581 tcaagatgtt cattaactac tacttttcct ctttccattt caattcctat atcttctact
    14641 ccagaattgt caaggttagg tcttctacca acacatacta atgcatattg tgcttctata
    14701 acctttccat tagaaagagt tgcaacagcc ttaccatcaa ctacttcaca agtttgtact
    14761 ccaatacctg taataacctt aattttatct ttcttaaatt gtctaagtag ttgtttagct
    14821 acatctttgt cttcatttaa tagtatttgg tcaaccatct caactatagt tacttctgtt
    14881 cccaaggctc taaagaactg ccctatctca catcctataa ctccaccacc tactattaac
    14941 atagactcag gtatctcttc aagtccaaga acttcatcac tagttattac aacttttcca
    15001 tcatatggga acattcttgg tacaactgga acagaaccat ttgctagtat tatcttatct
    15061 gcttttatag tttctactgt tccatcatct ttagttactt ctatagtgtt tttatcaatt
    15121 aattttccaa aaccattaac taagtttact cctctttttt caaataagaa ctctatacca
    15181 cttattaatt ggtttacaac tttatttttt ctttccatta tggcagtgaa gtttggctta
    15241 actgtaccat ctatttctat accaaaatcc tttgcttctt ttacagtatt caatactcct
    15301 gaagaagcaa gaagtgcttt agttggtata catcctgcat ttaagcaagt tcctccaact
    15361 cttctttttt caacaactgt tacatctgca ccaagcatag aagcttttat agctgctaca
    15421 tatcctcctg gtccaccacc tactactact atcttcatct ttaattcccc caaacctaat
    15481 ataatctaca atcttgcaat actataaacc tgcggcatcc agtatagcag gtatattctc
    15541 ttctgcccca attgccatta tatgaacacc atcacataaa tcttcttctt ttaattgttt
    15601 gataagttcc cctgccattt ttattccttc acttacccag ttttcttttc cagctgctct
    15661 taatctttcg atttgttcat ctggaacaaa tattcctggt acatttgcag tcataaattt
    15721 agccattcct ggtgatttta aaggtactat tcctgctaat accttgcaat ccatatccct
    15781 tgttaattct ctaaactttc tcattgtatt tatgtcataa agtgcttgtg tttggaaaaa
    15841 tttagcccct gcttttattt tctttttcat ttttaataat tgaacttcta ttggagaata
    15901 ttctggagtt actgatgctc ccagataaaa atctggagaa ccttttaact tattaccagc
    15961 catgtccgta cctgccatta aagtctcagc agtttgaagt atccccacag aatctaaatc
    16021 aaacactccc ttagcttgtg gatggtctcc tacgctagta tggtctcctg ttagtgccaa
    16081 cagattgttt atcccaaata cacctgctga caacatttct ccttgtatag ctattctatt
    16141 tctatctctc cctgttattt gaataactgg ctctaatcca gcatctttta ataatttaca
    16201 tgtagcaaga gaagttgctc tcataactgc cgattgaaag tctgttacat ttgctgcatg
    16261 aactcttcca actaacggct ttgcacattc tattaaatgt gaaaggtcag tcccttttgg
    16321 aggagccatt tcagtagtaa ctgcaaattt tccactttct aatgtttctc ttaataagct
    16381 catctctaat ccccctttta accttctaaa aaaaactttt atgaatttgc tgctgcttct
    16441 ttctttttct ttgcacttaa acttcttgga ttattttgct tagaatagtc ttttggtggt
    16501 cttatttctg ctaagttatc taattgtcct atagcttcta gtctttcata tatctttatc
    16561 caagcacaat cattctctga gtttacttca cacttaccat ttttagctcc accacaagcc
    16621 ccattcataa gacctttagc acacatagtt actggacata tcccacctgt ccaaccaagt
    16681 tcacagtctc cacaagcctt acaggcttct tcatattctc caactctttg tacttcccct
    16741 ataaacaaag tattgttggc tggataaact ggcttatctt ttaaattctt aactattgtc
    16801 tgtgtaccat ctccacatgc taaagataaa actgcatcag cttccttagt ctcttccttt
    16861 agagccttta aatccttttt agactttaaa aggttacaag cagggtcaag cattacatat
    16921 cctaagattt ttttgccttc accttcaaga gtttccttca tttttaaaac ttcttcttcc
    16981 ccaccacttt tacaagttgc tgcacattga ttacaaccaa ctaaaaccag cttgtcaaaa
    17041 tcttttaagt accctagcac ttcttctaat ggtttatttt cagaaattat catatctact
    17101 tcccccctaa accttccaca tatatatatc tatttattgt tttgtaattt acaagcctta
    17161 actacatgtt ttgcaagtat agatgttgta actgaaccaa cccccgctgg aactggagtt
    17221 atcattgata ctttgtctaa aactgcattt gtatcaacat ctccacataa atttccttct
    17281 tcatcaacat ttataccaac gtctataact accgctccat ctttaacaaa actttcatca
    17341 accatcttag ctctaccaat agctgctatt agcacgtctg cctctgctgc tacacctgat
    17401 agatttttag tttttgaatg acatattgta acagttgcat gttcacttaa taaaagcatt
    17461 gatactggct ttccaactac cattgatctt ccaagaactg ttaccttact tccttttaaa
    17521 tcaacattat agtgttttaa tatctcaact actgctgtag gtgtacatgg tggaaatcca
    17581 cttttatccc cttccattac cttagcagag tttattggac taaaacaatc tacgtctttt
    17641 tccggtgcaa taacatattt tataacttct tcatttaagt gttttggaag aggtctaaaa
    17701 cataatatac cattaacatt tttgtcatca ttaactctct ttaatacatc aatatattcc
    17761 tcttgtgtta tatcctcagc taactctaac acttctgttg ttatacctat gttttgacat
    17821 ctctttagtg ctcctctttc ataagataaa tcatcacttc ttgccccaac tctaactatt
    17881 gttagtttag gatttatacc ttctttaatt agtaaatcga cttcttttat taattcttca
    17941 ctaatcttat cagctacagg ttttcctttt ataatttgtc ctttagttga cattccttcc
    18001 atacattcac aactccccta caagcaaact taaattaatt tattctatat accacatatt
    18061 aataacatag tatctacttt cgtcatttaa aaattatttt ccaagagctt tttcaacttt
    18121 ggcataaact tcatcacaaa tttttacacc atcttcaact aacttatcta cttctgtttt
    18181 taccttatta acatattctt tatcttgtat agaatttata tttataacta catttaattg
    18241 tccaccaagt atagcagctc ttaaacactg aactcctaca ccaacatcac ttatagcaag
    18301 tcttgaacct ttatcaacta aatcttcatg taacttaata gcttcatagc aaactcttac
    18361 tatgtttaat ggtacttcac aagcaacttt taatgccttt tccattgttt ctgcttttat
    18421 tcttttttct tcttctgttt ctttaggaag cccgtaagct tttgataatg gtaagaaaca
    18481 ttctgcatca tcatctatca tttttaataa atctttttct aactttcctg ccttacttaa
    18541 tatctctttt acactttctt catactcagc atacttcttc ttccctattg taaggttaca
    18601 taccatacta cctaaagcca tacctattgc acctactagt gcagcagctc ccccaccacc
    18661 tggtactgct tctttggaag atagtacttc tacaaaatca acacaagttt tatctgctat
    18721 tttcatgata atcctccttg aaaggatgta ggaaaatatc tcaaattgtt cgagatattt
    18781 tacgaactat tttaagatat ctcctactct ttatttataa attaatttcc ttagaataat
    18841 ccagctatta ctccattttc atcaacatct attttttctg ctgctggaac ttttggtaat
    18901 cctggcatct tcattatttc tccagtaagt gcaactataa atccagctcc tgctgaaatt
    18961 gttgcttgtc ttacagttat tctaaatcct gttggtctac ctaatttagt ttggtcatca
    19021 gttaaagaat attgagtttt agccatacat actggaactt tagtaaatcc aagtttttct
    19081 aagttagcta tttccttatc agcttcagga gtataatcta caccatctgc tccataaatc
    19141 ttagtagcta tagcatttat cttgtctttt atagataaat catcttcata gcagaatcta
    19201 aagttatttt cttcttcatc caataatctt aaaacttctt tagctactgc aattccacct
    19261 tctccacctt ttgcccatac ttcagaaaga gcaacattaa cacctaattc cttacacttg
    19321 tcttcaacaa gtttaagttc agcttcagta tcaagtggga atctattgat tgcaacaact
    19381 gctggtaatc catatacttg agttatgttt tcaacatgtt ttaataagtt tggaagtcct
    19441 ttttcaagag cttctaggtt ttcgttattt aagtctgctt tagcaactcc accattgtat
    19501 tttaatgctc ttacagtagc aactattata acagcatctg gttttaagtt tgccattcta
    19561 cattttatat ctaagaattt ttctgctcca aggtcagcac cgaaaccagc ctcagttatt
    19621 acataatctg caaagtgcat agccattctt gtagctataa cagagttaca tccatgagct
    19681 atatttgcga atggtccacc gtgtacaaat gatggtgttc cttcaagagt ttgaactagg
    19741 tttggtttta aagcatcttt taataatgca gccatagcac cattagcttt taattgtcta
    19801 gctgttacag gttcaccttc gtagctgtat ccaactatta tgtttcctaa tctttctttt
    19861 aaatcagata tatcacttgc caaacagaac gctgccatta tttcagaagc aacagttata
    19921 tcgaatccat cttgtctaac tgctccatca ccttttttac ccataccatc aacgatattt
    19981 ctaagttgtc tatcgttcat gtctacacat cttctccaag ttattttctt agggtctatt
    20041 ctaagtgcat ttccttggtg aatatggtta tcaagcatag cagctaaaag attgttagca
    20101 gctcctatag cgtgaaaatc tcctgtgaag tgtaagttta tatcttccat aggaacaact
    20161 tgtgcatatc ctccaccagc tgctccacct ttaacaccaa atactggtcc tagagatggt
    20221 tctcttaaag caactaatac atttttgtct agtttagcaa atgcatcagc aacacctata
    20281 gtagtagtag tttttccttc acctgctgga gttgggttta tagctgtagt tagtattaac
    20341 ttagcttttt taccagtctc tctttttagt agattgtagt caacttttgc tttgtattta
    20401 ccataaagtt ctacatcatc ttcacctagt cctaattttt ttgcaacctc tctaatatct
    20461 tgaggcttag cttcttgagc tatttcaatg tcagatttaa atcccataac tgaatctcct
    20521 cccacaacct aaatagattt aaaatataat ttttgtaaaa attgtattaa tatgtaaaaa
    20581 ttatatttta taagataatt atacatgtaa aattacttaa aaactagcta ctaagctatt
    20641 aaaatattca aacttaatag ctagttattt atctaattac ttacttatta cctatctatt
    20701 gcctatgtct aacctttaac caacttaaaa tcccaaatca aaatttctat ctattttact
    20761 tatccttaag tgacattaat ttatctttta ttgctttaat ctgttctttc gttgtttcac
    20821 tagaatcata tggagattga ttagctctat cagaatcaat aacatcttgg ttataataaa
    20881 taaatcctaa agattctcca tcttctaagt tttggattat aaattcttca tcttccttat
    20941 ttctaatttt atttcctact acaaatactt tatttacacc tatatcatgt ccaagtttct
    21001 taacttttct ataagtctgt agacttctct ctcctggttc tacaactact ataaaagcat
    21061 ctacaccttg ggctgttcct cttcctaagt gttcaattcc cgcttccata tccataacca
    21121 ctacatcttt attttgtagt attaaatgtg agcagagtct cttcaaaaga acatgctctg
    21181 gacatacaca tccagtacca ccagaatcta cggttcccaa tgtaagtaat cttactccat
    21241 tgtattcttt acagaaattt tcagggatat cgtctacttt aggattcatc ttaaacattt
    21301 ttccaaatga acctacagaa gcagctgttc tatctgaaac taattttttc atttctgata
    21361 taggtactat tgattcatat acttcttttg gaaaacctag tgctaaagct aaattagcat
    21421 cagggtcggc atcaacagca acaactctgt atccgtcttc agcaaacatc ctagagagca
    21481 ttgaagaaaa agtcgtttta cctactccac ctttacctgt tattgctatc ttcataatcc
    21541 ctaccacctt ttattactat ttgcttttta gtagttaatt ttacagttaa atatttttta
    21601 tactctatat ttttatacac taaaaataaa ctacagctag tccttacagg aaccaattaa
    21661 atttacccta ctagcttttc aaagcagcat gtatagtttt attattcttt aatattgttt
    21721 atattttatt tattcagtat attcattata ttttgatgtt ctttacttta atatttgact
    21781 ttattcaact ttattcaacc agaaacttct tataaaaacg tttattctat aacttcacta
    21841 ttttttaagc ttcagccttt tctgccattt tttcttcaac taatgcttca aagtgaacac
    21901 gttttttctc tatatcttct attattgtag ctgctaactc ttttggattt gtatttacag
    21961 taaatgtagc tccaacttta tcttttaatt cactagttaa tatatccact gctcttgaag
    22021 aaccagctat tggaggcatt attcctaaat aagtgtctat accagaagct acaacatagc
    22081 atcctatagc aactgctttt tctgacatcc actctggagc tatacctaca actggaaggt
    22141 cacacatatc catatctaag taattagctg ctgctccaac taaatctagt atacggctta
    22201 tatcaacaca agaacccata tgtagtactg gtgggattcc tactaattta cagacagttg
    22261 caagaccttt acctgcatat ttttcagccg catcagattc cattaaacca aacttagctg
    22321 ctgctgaagc tccacaacct gtagtaacta ctattatgtc gtttttgatt aattctttca
    22381 ttattgttac gtgagcctca tttgaaactc ctttagcatt gttacatcca actacaccag
    22441 ctgctcctct taatactcca gcttttaaac agtctgttaa tggcttaact gttcctgctg
    22501 ggtctatatg agagtttaca actctgtcta attgatttac tactgcttct acactatacc
    22561 caactgttgc gcctgatttt aattcaggta taagtacttt tgatttatct ctatttttaa
    22621 agtttaaaat agcttcttta actattgctt tagcatcatc aagtgcttgt tcttctctaa
    22681 attcaatata agttgaacct gttatcttag cttttggaga agttgttaca aatttagtat
    22741 ggtaacaatc tgcaactcta gctaatgcag ggaatataca ttgaacgtca actattacag
    22801 cttcaactgc tccagttatt attgcaagtt cttgttgatg gaagtctcct gctattttta
    22861 caccatgtct cattgtaatc tcattacctg tacaacacat acctgctaag tttattccat
    22921 cagcaccaac ttctttagct aaagctacta agtctggctc ttctgatgct aaaactatca
    22981 tttctgataa tgctggctca tgtccatgta atattatgtt aactttattt tcttctaata
    23041 ctgctaaatt cgcctctgtt cttcttggca ctggagttcc aaataagata tcgcttagac
    23101 gagtacctat catagaacca ccccaaccat cagccataga agttctaaga ctcatgtgta
    23161 ttaaactatc tatatcacca gtacatccta tatgagtaga gtgcattatt gttgcaattt
    23221 ctctatctat agctcttggc tctatagcgt attctttcca tattttttgt cttggttctg
    23281 gtgcattttt tataaatctt aatgtcccaa aaggttttcc aaattccatt aatgcaattt
    23341 ctgccacttc atgtgctaca tcatagatat ctctaccttc tgtctctaca tcccattctt
    23401 tagctaaagt aattaactta gcttcgtctc ttattttata gtttccattt ggatcagcta
    23461 atgctaatgt atgtgctata tctctagcat ggtctgaatg ggcagctgtc ccaccagcaa
    23521 ccattcttgc aaagtttcta cctactatag tatgctcatc tgctccacat aacccccttg
    23581 gagtcttagg gcttattcta catggtccca ttccacatat tctacagcaa acaccttgaa
    23641 gaccaaaacc acattgtgcc ttaaagcctt cttttctatc atacattgtt tctacgcctt
    23701 cttctctagc tttagctatc atcttctggc tgtttaaatc aattgttaac attttttcat
    23761 ccatattcct atcccctttt tttgattttt aaaatccccc aggtgactga cttcatgtca
    23821 taaaataata aaataaatgc tttctaagtt gattatatac taattttcag tgctttgctt
    23881 tagatttaaa tattttttta acaaaattag ataatacata aaaattttat tttctacact
    23941 taatttaagt tgattttatc gcttgttttt ggctatttgt taatatctaa acctactttt
    24001 ttgatacttc agtttaatta aattatgttt tgattttttt aaatcaaatt tgaaattttt
    24061 ctagtcattt ttatattatt gcagattgtt tgtgtattta ttatctttca ctactgtttg
    24121 gacttattac agatttttac tccctaaact taattagtaa aaatttctta gaatagcatt
    24181 ttttggacta atttttttga atttttttat ttttcctact ttaatattaa ttatagcttc
    24241 aaattgaaca tttgtatata ttcatttgaa gctataatta ttttttatat aaaacttttt
    24301 taatacagca ttaatttaga cattttgact attttttcta ctttaattca tttatttcaa
    24361 gcgatttatc ctatttcata tttttagcta caatagcccc atatgcaata gaatataatt
    24421 tagattcatg tgaatcagct cttgacacta aaactatagg agcttttgca cccataatta
    24481 tacctgctga tttagagtct gccatatatg taaaggcttt ccctattcca ttaccaacct
    24541 ctattgttgg aaccaatatg atatctacat ctccagatac ttcactttta aaccctttta
    24601 tctcactagc ttcttttgaa acagctaaat caaaagccag aggaccttct acaattacat
    24661 tttcaccaaa ttctccttct ttacaagctt ttgctaacat atctgcatct acagttgctt
    24721 gcatcttagg atttactttc tctttagctg ccaaacatgc aacttttaca gtttccattc
    24781 ctaaagcttt tgcagctttt atagaatttt ttaatatctt tgccttttgt tcataatcag
    24841 gagctatatt catacctcca tctgtagtta taagtaattt atggtatttt tctaactcat
    24901 ataccataac atgacttaat aaactatcag ttctaagacc atagtcttta tttaacactt
    24961 cttttaatat gacagatgta tctaataatc ctttcattac aaagtcagct tctttacttg
    25021 atactaattt aactgctatt tctgcacact ctttgatatc atccttatcc actattttga
    25081 taccacttaa atcaaatcct atttcttttg atatttctct tattttgcct tcatttccaa
    25141 ccaatatagg agtaattata tttctttcta cagcatcctt tattgccatt aaaacttctt
    25201 tatcatgagc tgctgctaca gagagtacaa ttttctctcc tcccttaagt tgttctaaaa
    25261 tatcatctaa tttttttatc aaaagtaacc cctcccttat atatatttaa ctaaataata
    25321 ccatatttgc tatacctttt ctcaatttat tttttatgtt ataaagaatt tatataacta
    25381 aaagacagtt taatatttta ttctgggatt tcatcaatag ctcttaaaag ctttgcagga
    25441 acaccaccta caatagtatt ttctgcaaca ttttttgtta caactgctcc agcagcgata
    25501 attgcattat ctccaatagt aactcctgga actacagtta cattagcacc aatccataca
    25561 ttttttccta ttgtgattgg agaaggataa gttgtaccac gtttctctgg tgcaatacca
    25621 tggttaagtg tagccaatac cacattatgc ccaatttgcg tattatctcc aatctcaatc
    25681 cccccctggt cttgaaaatg gcaactgcta tttataaaaa cattttttcc tattgtgatg
    25741 ttttttccac aatctgtata aaatggtgga aacataaaaa atgattcatc tacctttttt
    25801 ccaattaact cagaaaataa actacggact tcttctggtt catgataaga agaatttaac
    25861 ttacttgtga ttttcattgc ctcattactc aacttaacca taaattcatg tatttctgat
    25921 ttactagaaa caggttttct ttgattgata tgtgttaaaa attcattgat attcatttta
    25981 tacatccttt caatatgtgc tttaatttat ttgtacattc taaataatat tttaataaaa
    26041 attaatttaa tttattttta ttaaaatatc attgcttctt ttagtgtaat atacaattat
    26101 atctatatca aatatctata ttaaatactt aactatactt aaaaagcata taaattgata
    26161 taattatata ataaataagt taatattgag gagggataat tatggaaata agagtattaa
    26221 gatatttttt agcaattgca agagaagaaa gtatatctgg tgcggcaacc tttttacata
    26281 taacacaacc aacactttct agacaaatta tggaacttga agaagagttg ggagttaaat
    26341 tattggttcg aggaaataga aaaatcacct taacaaatga aggtaggctt ttacataaaa
    26401 gagcagagga aattatatca ttagttgata aggttgaatt agaatttgac aatcccaatg
    26461 agattattag tggtgatata tatataggaa gtggtgaaac tgaagctatg cgacttatag
    26521 ctagaactgc ccatcatttt catcaaaaat accccaatgt aagatatcac ctttttagtg
    26581 ggaatgctga tgatgtgact gaaaaattag acaagggact gcttgatttt ggtattttaa
    26641 ttgaacctgc aaatatacag aagtatgatt atgcacaaat tcctgctact gatgtatggg
    26701 gtttactaat gcgtaaagat agtgcccttg catcacaaag cacaataaat ccaaaagatt
    26761 tatgcaatat acctcttatt atttcaagac agacacttgt tgataaagca atttctaaat
    26821 ggcttcaagg cagtaatgaa aaactaaaca ttattgcaac atacaattta gtttataacg
    26881 cttctctttt ggtagaagaa ggtctgggct acgctctgtg tttagataga ctcgtcaata
    26941 ctacagaaaa cagtaatctt tgttttagac ctctatctcc tcctttagaa gctaaattaa
    27001 atattatatg gaaaaaacat caaatatttt caaaggcagc agaaaaattt ttacttcaaa
    27061 tacaaaaaga tttttcttct atcatgttgg atacatctaa gttggataca cctaaagaaa
    27121 aataaatcat aataaaaagt gagagtgtct caaaatgaac tttgcactgt ataaggcact
    27181 cttttttata caaagtaaaa tatatgttca tcatttcagc actaaaaaag aggcttatct
    27241 ctattttgag atagccccac ttaaccaata aaattaaata caacttaaca atatccttac
    27301 ttatttattc caagttctct tagacaagca atcgtaattt catcttgtgg atttaattca
    27361 taagctcttt ctatatagta agttgcatca tcaatatcac cattatttaa ataagccatt
    27421 cctaaattgc aaagtatttc tgggtcttcc tttatcttag ccgcttgttc aaaatactct
    27481 attgctttat ctaaattatt cttataagcc tcacaaagcc ccaattccac tatagtatcc
    27541 acctgattag gctttattat caatatcttc tcaagataca tcttagcctg ctctatttcg
    27601 cccataccct tatatccaag tgctatcata aacagcaagt tccaccaatc catatgttct
    27661 tcttctaatg gaagtaactt ctctaatcct tccttaaact taccttggaa tacaagtgta
    27721 tatccttctt catattgaac tttaaagtcc atcttgccca gattctcttg tacctcagct
    27781 actaaatcag catctaaccc tagccttaac gcttcttccc aagtcagctt agtctttaaa
    27841 tattgacctt gattataata atggtagcct aagtgataat aacctaatgc aaaattctca
    27901 tcaacatcaa ttaccttttc aagcttagcc atggcttcaa gtaagaatgc attcattgcc
    27961 ttatcatcca tatctttttg atactgttta gctatctctt ggcaaacaat cgcatagtta
    28021 tacattgcat ctaaatcatc tggatacatt gttataaatg cttttatata tattaaagaa
    28081 tctgtatgtt caccaatctc aaagtatttt ctcgacatat atcccatata cgatttcaaa
    28141 tccaaatcaa tatcttttga taaagcattt aaaaacttct catactctgc attgttttta
    28201 aacttactat ctattccaat tatatatatc attgcatctg ctatagacat tgaatttaac
    28261 ttatcttgag cagtcttttc ttttatccct tttactaata cctcattttt tataggtaca
    28321 tctaatccac cttttggcac ttcatagctt tttaatttaa actctccacc atgttttata
    28381 gttataaaag ctaattcttc tgcttttttt agcaaatatt tttctatctt aaaatccatt
    28441 ttatcctcct aaatacttaa atctctactt tatgagtttt agatttctca aatatttttt
    28501 tatcatcata ctcaaataag actacatttt ctacttctct tatttctgta tttatatcat
    28561 tttttaaatc cacaacatta aatctaaatg gctcaaaatg aacatatttt attatatgtt
    28621 tagctggttt atcaacaaaa ctaggtaaat atgttgataa gttatcatta tcttgtaaaa
    28681 atacatggca tctattctta aaatcatcca catctagctt attaaaaaac tgtggtatat
    28741 ttgatgtttt acccagtgat atatagtcat attttaatat atcgttgaat acatcattat
    28801 ctatgttgat tttttcatta tagaaatcca tcaatatttt atataactga ttctttcctt
    28861 gtgccaaatc aaagtagcca ttttcatcaa aatacttggc aaaaacctca aagaatttaa
    28921 aagggctttt tttataaaat ctgcctataa tatatctcat agataacaca aagtttttag
    28981 aattgtagta tctctccagt atatcttcta tatcctttag cttcaaagtt tcttcatagc
    29041 ttatataatt attgtataaa aactcatatg gagcataatc cttatattta aaaccatgct
    29101 catctgcaac ttttctcata cctgtccctt taatcatctt taaaaagcct aattgcagat
    29161 gctctatacc tagattaaat acatcattaa aagaattttc aaagctctta taatcttcat
    29221 aaggcagtcc tgcaattaag tctaagtgtt gatgtatatt tctataagat gctacagttt
    29281 gtacaacatg agacaacttt ttaaaatcat cccttctacc tactgcatca agtgtttttt
    29341 cattcgttga ctgaacccct atttcaaatt gaaaaagacc ttctttacaa tcagccaaaa
    29401 actcaagcat cttatcatct atcaaatacg ctgtaacttc aaaatgataa gttgtatatc
    29461 cattatcatt tttcataaga aaatccatga tttccatagc cacttttcta tttgcattga
    29521 atgtcctatc tataaacttt atttgtgata ctcttgcatc tattaaagct tttaaatcac
    29581 tctttatcct gtctaaacta aaatacctta gacctggaat agtagatgat aaacagtatt
    29641 ggcagttaaa agggcaacct cttgatgatt cataatacac tattctattt tcatattctt
    29701 ttggattcaa gttttcataa ggactaggaa tctcatccaa atcttgaagt aattccatag
    29761 gcttattcac tattatctca ttgccttgtc tatatacaag tccatttaca tcatttattt
    29821 ccatctttcc ttgcaagtga agtactaaat ctctaaaaat taattctccc tctccataaa
    29881 gtatataatc cacaaaatca tactttttca ttgcctcata actatcataa ctcacttccg
    29941 gtccgcccaa agctatttta gtattaggtc taattttttt tagattatca caaagtttaa
    30001 ctatatctcc cacattccag atatatgttg agaataatat tatatcatat tcatttttat
    30061 atatatcctt taaaatatag tccaaatcgt tgtttatcgt gtattctttc atattaactt
    30121 ctatcaaatc tcttacaaat tcttttagat atcttatagc caaatttgta tgaataaact
    30181 tggaatttaa tgttgttaat aaaattttca aatatttctc acctcttttt aagattatac
    30241 tgtctttttg taccccttat aggttaaaat agtatatgta aagtctaaat aatatctata
    30301 taattaattt attaatttca agtaatgtga ggaaaggaag ttttttatgg aattaattga
    30361 aaaagtatct atcaactctt tatcaaaaag agatttacta cttattataa agtctcttga
    30421 atacactaat gaaaatactc aaattagtga tttcattgac cttaggaata atatagtaaa
    30481 ggaattatgt tttttaactg acactaagga aaaagatttt ttagattatc tagaaaaaaa
    30541 tagcacactc taataaccta aaccttttta aactttaata tataaatacc tgatttagaa
    30601 tttattttta tgcttaatat attatcatta aaggttttcg acataactgt tatttgatta
    30661 aatatagggt ctactatagt attaccttct tcaagtctta taagacttct tgggtctatg
    30721 cttttattat atgccttaac tccaatttta tattttctca atttaacaac atattcggac
    30781 atagaaaaaa taaaatctat attatcttca tttaatttac tatcttcacc caaaatggat
    30841 aatatagatt tttttatgct attaattcct tcattttcta cagttctatc ttccatatat
    30901 acatcatata gaacatttaa aacatttaat aaaatacttt cttcatcata atcttcagat
    30961 ttcaaatcaa tgtacttatc aattaattgt attgctttaa tccttgccat ctgaaacttt
    31021 acaacatctg atttgtctaa ttctatagaa aatcctatta tcttgtcttt aatactctgt
    31081 agtaattcga catactctat tttactatta tttataagat tgtgcataat ggaattatac
    31141 accacagaac ctaataaata atcaaataaa agttcttctt ttttaaaata aataacatac
    31201 ttttttatta gtttaataac ctcatcttca cttttttgaa tattagtatt tgataaaatt
    31261 agtggtaata aaatatttaa aaaattgata tttcctattt tttctccata aaatctatag
    31321 taaataagtt catctctcag tctattttcg tctatataca cacatctttg cgttttatca
    31381 tctattaagg attttgtatt tattatagtt aaatctttaa gagccctact tgcactacta
    31441 aaagaagatg tgtagacttt ttttagtaat tcgtcattca taaatatatc ctttctgaaa
    31501 aattgaatgt aaataaattg ttattaaaaa aaaagagaca gcccgtttaa gaagctgtca
    31561 tatatagggg gatagtagtg tacaaaaatc attgtaatct actatctata tattattata
    31621 cgatactact tttttatatg taaagcatca gttttcgaca tccctatatt ttacagttat
    31681 acaaattgcg tactaagttc aattttattc tttttatttc aacattttat catgctacat
    31741 tctttcagga gcgtgcattc ccaacaattt cagagaattt tctaaagttt gttttgttgc
    31801 taaaactaat gcaagtcttg cttttcttat ttccaaattt tctactaaaa ttggattgtc
    31861 atgataaaat ttattaaacg cttgagctaa atctagcata aatctagtca ctatatgtgg
    31921 ctcatttttc tttagagcaa gtaaaatatt cttattgaat gattctataa ctctaagaac
    31981 ttctgcacta tcttcatcag ctaatagact ataatctata tcagaagtca cttctacttc
    32041 tgcttttctt aaaactgcac agcatctagc atgtgtatac tgaacatatg gtccagtctc
    32101 accatcaaaa ctaagcgttc tttcccaaga gaaggtatag tctttaattc tactattaga
    32161 taactcttgg aatactactg ccccaacacc tacttgctta gatatttcat ccacattttt
    32221 agcatttggg tttttagcta gtataatttc cttagtttta tctatagctt gatttaaagc
    32281 atcttctaaa aatactactc ttccttttct agtcgacata gtaccttctt caagtgcaac
    32341 cataccaaat ccaacatgaa ttaaatcttt tgcccaatca tatcccataa gctcaattac
    32401 cttaaaccat tgttggaaat gtaagttttg ttgagaacca actacatata tacacttgtc
    32461 aaaatcataa gtttcttttc tatatattgc agcagctaaa tctcttgtca tatataacgt
    32521 tgaaccatca ttcttagtaa taagtgctgg tggcatttta tactcttcta aatcaacaat
    32581 tcttgctcct ttagactctt ttaaaagatt tttctcttct aatagttcga taactctgtt
    32641 cattttatca gaataaaaac tctctcctgc taaagaatca aattctatat ctaaaagttt
    32701 ataaactctg ttaaactctt ttaaactctc atttctaaac cattgccata attcttgtgc
    32761 ttcttcgtca ccattttcta atttattaaa ccatgctctt gcttcatctt ccatttctgg
    32821 gtgttgttca gcttcatcat ggaatcttac gtataatttt agtaattctg gaattggatt
    32881 agactctact acttcttttt ctccccactt tttaaaggcc actattaact taccaaactg
    32941 agttccatag tcacctaaat gatttattct tatagtcttg tatccttgcg aatcatatat
    33001 cttatataaa gcattaccta taactgtagt tcttatgtgc cctatatgga aaggttttgc
    33061 tatatttgga gaagaatatt caactattac agttttatct tttccaaaat cactatgtcc
    33121 gtaattttct ttttcatcta aaactttttt aattacagtt tctgctaatt gagatttatt
    33181 tacaaagaaa tttacatatc cgcctaattg tattactttt gttatctctc cacttggttc
    33241 tatactttcc gccaattcac ttgctatcat gtttggagct tttctaaaca ctttggctaa
    33301 tttaaagcat ggaaaagcat agtcccccat atccttattt ggtggaactt ctataagtgc
    33361 ctctatctct tctttcgata aatcttctat tttttcttta aggcaatttg atattgccac
    33421 cttaaaatct tgcattctgt tgctcctctc tacattgttc ctggtttata ttgtgcttat
    33481 ctctttatgt taaatcaaat ttcgtgtttt ttcaagtatt tcattacagt gtaaaacact
    33541 ttagtaatta tgttttctgt atatattaaa aatactataa tttcagtttt taatcacact
    33601 attattgatt ttttataaga cttttttcta ttttattgtt tcttgtacta tccagttgac
    33661 tttatatact ttttttaatc ttttatctgt tttcatacat caatgtcagc tttatataca
    33721 tttttttaat cttttatcta ttttcataca tcaatgtcag ctttatatac tttttttact
    33781 tttttatcta tttttataca tcaatattag ctttatatac tttttttact cttttatcta
    33841 ttttctataa atctttttca ctcttaactt ttattccttc ttcttctaat agccttgctg
    33901 ttataccttt ccctggtatc tttttacctg aaaaactacc atcatatatt agattacatc
    33961 cacaagaggg gctaccctct tttagtattg cttcgctaca tacatataaa tttgctattt
    34021 ttagagtttc attagcccct cttacaaagt tttctgttac atccttatta tctttagtaa
    34081 ttacttttcc atcaactatc tcagcaggtg gtcttggtgt agatagacca cctaactgct
    34141 ctgggcatac aagtgtaaat tctttgcctt ttaaatattc cttgactttt tcattatcat
    34201 tattatcgcc actatattta caatttattc ctattaaaca tgcagatacc aatatcataa
    34261 aaatgtgctc ctatacaaat tatatatatt ctcatacaaa ttactaatgt attcttatac
    34321 aaattatttt aatttaacaa ttgttactcc tgctccacct tctccatatt gaccacctct
    34381 ttgtgatttt acatgtctat ttcttcttaa aatgtcttgt agccctgctt ttaatactcc
    34441 tgtacctatt ccatgtatta ctgttacaga ctctaaacct gccacatatg catcatcaag
    34501 atacttttct acttccatta tagcttcttc taggtttaac cctctaaggt caacttcatt
    34561 ttttacacta ccagattttg accttataat gtttctcgtt gatttagtta catttgttga
    34621 aacatctttg cgtgtttttt gtaaagattt aaaaggtaga gtcatcttca ttataccaat
    34681 ttgcactact gcttcttttc tctttttatc tactgaaact acactaccat tttgatttaa
    34741 agttattacc ttaacttctt cacctggctt taagtctttt atctccttat ttgatacctt
    34801 tggaactatc atacttttta ctgttggctg aagacttccc atagagctag tcaattcttt
    34861 tctaagttct tctatcttac gattcttttc ttttgaagct ctttcttgtt caagacttct
    34921 aagttctttt attataatat ctacttcttc tttagcttgt cttataattg aaaatgcttc
    34981 tgatttagct ttttcaatca tcttatctct ttgactgacc aatttttcaa gtttttcgtc
    35041 atactctact ttcagttttt caatctcttc ttttaatctt tcagcttctt ctctatcttc
    35101 tactgctttg attctattct tttcaacatt ctgtaacaca tcttctaaag ctatgttttc
    35161 tgtatttata tattctttag cacgtgaaat cacataatca cttagcccaa gttttctaga
    35221 aatctcaaac gcatttgatt ttccgggaac acctataagt aacttatatg ttgggcttaa
    35281 agtttctata tcaaattcaa ctgctgcgtt ttctacacct ggtttagtaa gagcataatt
    35341 tttaagctca ctatagtgag tggtagctat acatttagct cctacagaat ttatatcttc
    35401 taatactgct attgcaaggg cagctccttc aacagggtct gttcctgctc ctaactcgtc
    35461 aaatataact aaagagtctg ctgttacgtt ttgtaatata gatactatat tcgtcatatg
    35521 agatgaaaat gttgatagac tctgttcaat actctgctca tctcctatat ctgcaaatac
    35581 attatcatat acacacatac tacttccata atcagcaggt atatgaagtc cactttgtgt
    35641 cataagtgca aatagaccta cagtttttat tgtaacagtc ttaccacctg tgtttggacc
    35701 tgttatcacc aatgtatgga agtctcttcc taaataaatg gtatttgcta ctaccttctt
    35761 tttatccagc agtggatgtc ttccattttt tatgtttaaa tatttatcct catttaaagt
    35821 tggttctatt cctctcatct gtatagaaag ttttcccttt gaaaatgcaa agtccaatct
    35881 tccaagtatc tcttgattag agattaaatc ttcgctcact tctccaacca tagcagaaag
    35941 ctctgataaa attctttcta tttcttcttg ctcacctagt tttaactgtc tcaattcatt
    36001 gttcatctct actatagtca ttggctctat aaaaagtgtc gcaccagaag atgactggtc
    36061 atgcactata ccagctactt gcgacctata ttcagatttt acaggtacta caaatctatc
    36121 acctcttaga gaaattattg catcttgtaa gtacttttga taagtagttg aacttattat
    36181 tgaatttaat tttgacctaa tggattgatt tttttgggct attcttcttc tgatatctct
    36241 aagtgtactt gaagcattgt cagatatttc tacttcacta attatagcat tgtatatctg
    36301 gtcttctatg tccttgtata catatagaga atttgataga gattgtatta ttggataatt
    36361 aaaatcttct tcatcactac tagataagct atttcttaat cttctagcca ctcttaaggt
    36421 atctgctatc ataatcagac ttcctgggtc aagagaagca cctatataag ctctctttac
    36481 cttatcttct atatcatgaa taccttccaa ccctactgaa cctctcttta taataattga
    36541 ttgagcttca ctagtttctt ctagcataga tttcacttct acaaaatcag tatttggtac
    36601 taaattttct atatatttta gacctaaaga agatgaggct ttcttcttta acaaatctat
    36661 tattttattg tattctagaa ctcttaacga tttctcattc attttctata ttcaccatcc
    36721 tcgatatttt aactttttta tttaatttta aaaatgtatg ttttcaagca tttccaataa
    36781 aaactgcaag ttttatttct ttttcatttc taaaattatt atattttttc tcttttagat
    36841 atattttaca catattttgt attatactat gtatgtagct atctatatat tatacataaa
    36901 catgctttat ttgtaatata atctgttaaa aaatcttctt taatattttt aaaggagttt
    36961 attttagctt ataaaaaaaa atagctactt atatagataa attaaggagg aacatgagtc
    37021 atgggaaacg tattagaagg attaaaacca gaaagtgttt ttaaaaactt tgagaaaata
    37081 tctcaaattc ctagaggttc tggaaatgaa aaaggcataa gtgatttttt acttagcttt
    37141 ggtaaaaatt taggtcttga aactattcag gatgaaagct taaacataat aataagaaag
    37201 cctgctacaa aaggatatga aaattgtcca ggtgtagttc ttcaaggaca tatggatatg
    37261 gtttgcgaaa aagaaaagaa tgttgaacat gattttctta aagaccctat aaaattaaga
    37321 attgatggtg acatgatata tgctactgga actactctag gagctgacaa tggtatagct
    37381 gttgctatgg gtatggctat attagaagat aatactttag agcatcctgc actagaagtt
    37441 ttagttactg ttaatgaaga agatggtatg aatggtgcag atgcactaga ccctagctta
    37501 attaaaggtc aatatatcct aaatatggac tccgaggagg aaggatactt attagttagc
    37561 tgtgctggtg gtaaaacttg tgttgttagt ttacctgttg aatacaagga agttaaaggt
    37621 gacaaacaag gattattagt tgaggttact ggactattag gtggacactc tggtatggaa
    37681 attgttttac aaagagcaaa tgctaacaaa gcaatagcta gagtattaag tgtattgaat
    37741 gttgattatg aattagcttc tgttgatggt ggtactaagc acaatgctat acctcgtgaa
    37801 gctaaatgtg taatagctgt taataaagct gatgttgaat ctgctaaaaa acaaataaat
    37861 gacattttaa ctgcatttaa acatgagttt actacttctg accctggcat gacttactca
    37921 gtagctgaaa cttctgtaga caaagttctt actaaagatt gtaaagaaaa agttgttcaa
    37981 atgtcttgct taactcctca tggagttcaa tcagtaagtc ttgatataga aggattagtt
    38041 gaaagttcta ctaacttcgc tataatcgaa actaaagaat ctactattga atttttaact
    38101 tctgttagaa gttctgttat gtctattaga gatgaaatcg ctgatagaat aagacttttg
    38161 gctcaagctt taggtgctaa ttacgacctt atagctcaat acccagcttg ggaatttaaa
    38221 aaaggctcaa aattagaaaa aatatgcagt gaaacttatg agaaattaac tggtaaagtt
    38281 cctactgtta tggctctaca tgctggtctt gagtgtggat tattacttga taaacttcct
    38341 catgctgaag ctatctctat aggaccagat atgtttgatg ttcatactcc aaatgaacat
    38401 gttagtatcc cttctgtagc taatgtttgg gattatgtta ttgaaatatt aaaatctatg
    38461 aaccaatatt aaatttaagt tttgattcta agtagatttt aattttaagt aatctatata
    38521 aaattaactc tgtaaattaa tactacggtt agtataatat ttactttcaa tattaactta
    38581 tagaaataaa gcctaatgat agataataac atgtatcatt aggctttatt gtaatttaaa
    38641 tattattaag ctttattctg ttttaaaatt aattcttata ctattttaaa tctatttctt
    38701 ccttgtctct tagaatcata aagagcatta tctgctattt gatacagttc atcatactct
    38761 tgaccaggct ttgctataac tacccccata ctaatagata aactttctcc atttggcaac
    38821 ttaatgagat gaatatgatt gtatacatca ttacatttat taataagttc ttccttattt
    38881 attatatcat tgatataaac actaaattca tctccaccca aacgacctac tatatcattt
    38941 tttgcaaaac tttcatcaag tgcctttgca acatcaataa gtactttatc tcctaacaag
    39001 tgaccataag tatcattaat cgatttaaaa taatcaatat ctataatcaa taaagcacca
    39061 tttttttcag ttgataaatt caacaaattt tgtgaagtta attttctaat atatgctgca
    39121 ttataaatat tggttaaact atccctttga gccttatcta acaatctatt cttttcctct
    39181 ttttcagaat caatattaca tattttcccg attacataca ttggtatttt tctatcatta
    39241 taaattattt ttgaagtaac tctaatccaa tgaaaagtat catcaagcat aatagaatac
    39301 atttcagttg ttccattttc ttgtgatttt attacatcaa aaaacttttt attatattct
    39361 ttccttattt tatctctttc attttctata ttgccatttt cactttcctt atcattttta
    39421 tccaaaccat aataagtctc aatacccttt tctggcatag aaattatcaa tttatcttgc
    39481 ttataatcat actcaaaaaa gtattcactt gtcatttcat acaactgagt atgttttttg
    39541 agttctaaag atatcttctc gttcatactt gtacgagtat gtaaaccaaa tgccataaat
    39601 ccaataatac aaattaataa actggctaca ataaaaatag tctcaattgg attctgttgc
    39661 ataatataaa gcaacgagaa atcttgtcta tatgtagtat tttggtagac aattgattgc
    39721 attttttctg atgaaatact tataatagct ttattaaata tattcaataa ttcttgttta
    39781 tcttttttaa ttataccaaa acaaatactc tttgctttat aggtttgagg aactagacgc
    39841 acatttttat acttaggttg attcatatag tactgcatag aataaccttc gccataagtg
    39901 taatctgcat agcctttatt aacagcttct atacactctt ctaaattatc ataccaaact
    39961 atattctctt caaactgacc ataataatta atacctttgg ctagagctac acgttttcca
    40021 tctaaactat tctcatttaa tttattatta atcatcatag tatattgagt agaaagaaaa
    40081 ggccgagaca tagctatatt atgttcacga gagtattcat aatcataaat cattcctgct
    40141 acaatatcaa tttcacgctt tttagtcatg ttttctaaat cttcttgaga ttttacaggc
    40201 acaagttcaa attctaagcc tgttttatca catatatgat ttaacaaatc aattgatata
    40261 cctcttaatt cacctgtatc tttatctata ttttgaaatg gtggtttgtt taaaagtact
    40321 cctactttta atttttctgt ctctttaatg taattagttt cttcatcaga taaaaacatt
    40381 tgattattct ctggtgtaaa gtatttttca tataaagtag ttgaaaaata tgggtctttc
    40441 tgttcaatat tattaattgc tgagttaagc ttttgagtta aatttgtact acctttagga
    40501 gtaatgaaat aaaatggttt tgatgaaaat tttgctacag tacgtacacc ttctattggg
    40561 ttcatactca cattaagaag tacatctgct ttaccactct ttagtgcttc tagttgctca
    40621 tcatttgttt tacaatatac caattcaggt gacataagat tcattttaca atactcatct
    40681 aattctttaa gtctagtatc agaattttct attacagcaa tacggatttt tttcacatct
    40741 tgtgaactca ttatgttatc aatatctgta ttttcatata atgcttgtag tactgtattt
    40801 gctactccat aattattccc tgcataatta tatatttttt gcattggttc attgtataac
    40861 atacctccca ttatatcaat ttcaccattt tctaacatct tcattaattt tgtaatagat
    40921 tcatttatct ctccaggaac ttgaacaaat tcataattcc atccagtata ctgtgcaatt
    40981 tctagtaaat attcatatgt atacccaaaa tagttacctt gctcatctat ttcagttaaa
    41041 cctttttgaa taggataagc aacacgaatg gtacgattat cattacctat agcgttgata
    41101 ccaaaagaaa atatgcaaga agtgaatata gatgtaatta caataattaa taaaagtaat
    41161 ttttttaaat tcactacaca atcctccata tagttaagat ttacagtata aattactgca
    41221 taaacttgta gacatgtaaa aatagattag tatataaaag gatatataaa tctacttaga
    41281 aatttaaata tccttaatat atacaatata tttaaattat aactcattta tatgtctatt
    41341 acaacaaatt taccctgcta aatacctact ctatatccca taattttaca agttgtagtg
    41401 aagtttaagt tctatctcac ctcaaattta gcagtgtttc tatttacagt tcttttatat
    41461 ttcacatctg gataacccaa tataaatgaa gtgataactg cttcgttctc gctcattcct
    41521 aacagttctt ttatctttgg ctctacattt gatgccataa caaaaaatcc attataacaa
    41581 attcctaatc cttgagaata ggccataagt tccatattag atgcagccaa tcctccatct
    41641 acataagcac ttccacctag agacatatca cctacaacta ccataagtag tggggcatca
    41701 taaaataaag aatcatttcc gttctctttg tatcttttgt acattttctt aaatgtattt
    41761 ttatagatag aacgtacagg gtcattgtca tcagtatcta atgccaaatt ataaagacct
    41821 tgtattgcca gttctttcac ttcttttagc ttttctttta ctaatatgta tcttataggc
    41881 tgtctattac cacctgttgg tgtgtatcta ccagcttcca ctatgttttt tattttttct
    41941 tcttctacct ctatatcttt atattgtctt atacttcttc taaatttaat aaaattcaat
    42001 aacttttcgg gttctaattc aaaactttca cgattgtatt ctattacttc atttttatca
    42061 aatccttgca tttctattgc atctgttggg caaattgcca tacagtgacc acagttaata
    42121 cagagaatat ttttaacctt tgctttatca tccttaactt ttattgcatt tacaagacag
    42181 tctgattcac acatcccaca gcctacacat ttttctaaat ttatgtctat cattccaaat
    42241 tattcctcct tttaaaaaat ttactttagt ataaaacaaa tagttgtcta agtcaatttt
    42301 tattttttta acaatatact agccttaact acactttaat ggagaaaaat atacataaat
    42361 tggaacttaa attagaactt attcaaagta aaagataaaa gaagtagatg tctaaaagtg
    42421 aactttttag ttaattagat attctttatt gtatgaaatc aagtggatat ttatcatttc
    42481 aacaataaaa aaggcttacc tctattttgg atatgtccct gtcaagtaga cagactaaaa
    42541 aaaggctatc tttatgcact gagatagccc cttttcccaa ctactattgt ttatcttaat
    42601 attagtcaat aaatttaatt tgctataacc tgtaagttac attttaattt gatgtaactt
    42661 taaattacat ttttaagtaa tatctttaaa ttatattctt aaaatatatc attaaactac
    42721 attttaatta atctaaaaac tatatcttaa ttaatctaag aaacttacat tataatcttt
    42781 tagtttccca ttatcatata caatctttgc actaaagata tttccagtct tagcccaatt
    42841 taagaacagc ctgtcacctt cccacatatt taaatcgaat attttatcat tatcaaccca
    42901 aattaaattt ccttctttac aatcatttaa atctttttca gataattcac cttcaaactc
    42961 atctgagaaa tacaaaaaca tataccaatc ttcttcatct ttaaattttg gaaaagttat
    43021 caacccttta tatgacaatg atttaagagt aagaccagtc tcttctttta cttctctaat
    43081 tacacattct tctggagtct ctccttgctc aaactttcct ccaactccta catacttacc
    43141 ttcatgtata tcatctttct ttttatttct gtacaacatc aaagtcttgt catctttttc
    43201 tatataacaa atagttgtaa gtttcatgta tctcgccttg ctttctcttt tatattctca
    43261 actaattatt ttcagcactt tcaagcacat cacttatatt cttcattgca ttaagagaaa
    43321 gctctgcaaa ttcatcaaat ggaatcccta tatcttctat acttctcatt gcatctctat
    43381 ttacatttga agcaaatctc gtttctttca ttcttttaat aacagactta gtctttacac
    43441 tggtaagttt tttatctgga taaactaaag ctatagcagt tataaaccca gttatagggt
    43501 ctgctgcata tattgctttt tccattaggc tagttcttgt aactcccatt ccatcatgat
    43561 gaccaagtat tgctctatac atttcttcgt taccgaagtt ttctttttta agcagttcta
    43621 ctgttgttgt agcatgtcct tcacatattt ctcctggctt tcttccaact atatctgaat
    43681 ccaaatcatg taaaagacct gccactgccc actcttcttc tttttctggc tctagtcttt
    43741 tggctaattc tctcataaca gcttcaacag cataacaatg ttttctcatt ctatcagttt
    43801 ctaaatactt aaataaaatt tctaaagctt cttctctcat tttcaccaat ccccctgttt
    43861 tttcacatat atgatatttt tatatattaa tacacataaa ttcaagcttt tatatatatt
    43921 catgtatatt tttcatgtta tatctattct acacctttat agtagtgtcc tgcaactgtt
    43981 ccaatttcat ctaatttctt atacaagtct gtagcctttt ttccaagttt ataattatta
    44041 ctcagtacct caatgtgagc ttcaataacc tttttaacca tctcactatc ttcaactgta
    44101 aaatctatcc taaatacatc tattccaatt tcctctaact catatagatt gtccaacata
    44161 caagtaacct tcgaattgta aattgtactt ctacagaata tatcttgtgt tatcctataa
    44221 cttgcatccg taaagtctct taaagcataa ctactttcct tacatttagc aactctcttg
    44281 tcctttttac aatctctaac aactactccc ataggacagt attctgtaac catcatagga
    44341 gcatatccat atattactgc ttcaatttct acatctgtat attttaaagt ttgtttaatt
    44401 tcatttaagt ttagttcttg tgacaaacaa actgtacttg ctccctcttc tttgaagtga
    44461 tttatacttt cactattgaa tacatttaag tagcaatcta tatttaagtc ttttttctta
    44521 aagtattcca tagagcccca gttaccaatt tgaaccttat taattccttt tatattatcc
    44581 aatatctcaa attgcttgta ctctttattt ctaacaatcc ttggtgctga atagattaat
    44641 tttttgtcag tgactgctgt attagagtgg ttcttattca aagtatctat tttacttgta
    44701 tttgacaaag tatctatgtt atttgtattt gaacccatta gttctttagg agtacctaaa
    44761 gacgaagaag tatctaaatc catagaaata cttgaatcta tagaaatatt tgaatctata
    44821 gagatacttg agaccatttg taaagctttt gataatgtat ttatatcttc ataatagatt
    44881 aaatctacat tataatttaa tactgcttct aattgctcca agttcttaac tttaactcta
    44941 atcttaggag ctgtatcaag ttttttctgt ttatttactc tatttggctg gtattttaat
    45001 ctattcttct taaattttct atcttttatc tttattcttt cttcatctaa aagctcaata
    45061 caatcccttc tcatttgatt taacacactt ataggcatac ttacaccttc atccaggtca
    45121 atttctatct cttttaactt atatggtgta tttccaagct tttgaatttg tgtttctatt
    45181 ttttctttac ttaaagccac ctttatagct ttttcaacta ttttgtctcc ttctaccacc
    45241 gatttatttc cacaaaaatc acttataaaa agctctggct ttttacctag tttaatactc
    45301 acttttgcac gtatattagt ttttatgaac tctttatctt gctgaaatgt agcttgtact
    45361 ctatccatta attcgctgtc agaagtctta tatataagct gatttctctt tgcttctcca
    45421 acaaaatcta attctatcaa atccccttta tgtgctattg tagttatctc tccatccttt
    45481 attattcttc caatagtacc tccacctaag ttaattccat cacctttctt aagcgtattt
    45541 tctagcatta ctctaagtct tttgttcttt ctattataat ctaaaacttt tccaatatat
    45601 agcccttgat tattaggtct attagagttc attatatcct taccaacttc acctaatata
    45661 tatccttttg taaactttct attaaatatg gtatacaaat tatttattat ttcctcagaa
    45721 atacttattt ttttcttatc tacaaattca ttaatcgctt ctctatatcc tgaaaccaca
    45781 gtagctacat actctggctt tttcattcta ccttcaattt ttaaagataa tacacctgct
    45841 tcaattattt tatccaaatc ttctatagta cttaaatctc taggactcaa caaataatct
    45901 ccattacttt ttactacttc tcctgtattt atatctatca actcatactt ttgtctacat
    45961 ggctgtgcac acctacctct atttccagac ctattgccta tcatactact cataaggcat
    46021 tgacccgagt aacatacaca taaagctcca tgtacaaaaa cttctatatc tacagttgta
    46081 ttttcacaaa tccattttat ctcatcaaca tttaattctc ttgctaaaac aactctatca
    46141 aaccctacac tctgaaggta ttttacatct tctaaggaat gtgctaccat ttgagtgctt
    46201 gcatgtagtt caaagtcagg taatagtttt tttataagtc ttgccattcc tatatcctgt
    46261 actattaaag catcaatatc tatatcgtat aaaaatttta catactctac aaaatcttct
    46321 atttcatact gttttatcaa tgtatttaca gtaacaaaga cttgcactcc ccttatatga
    46381 gagtatttaa cagcctcaat cagctcattt ctatcaaagt tatttgcaga tgctctagca
    46441 ctaaagtctt taccacctaa atacacagca tttgctccgt tttgaactgc tgcttttaat
    46501 gaatcaaaag agcctactgg tgcaagtaat tctatttttt tcatattttt ctcctctatt
    46561 tatacttgtt aggttaatat aattatatct ataacttaaa tatttgtatt aaatttatat
    46621 atttcttact atattttaaa ttcttcaatc aatttatata ttagttattt atatttggtt
    46681 catttgagtt aacatataca ttatttacta tatttagtat atttctatta acttatatat
    46741 tgattactgt attttaaatt cttcaattaa tttatatact gcttactaaa tttaaaacat
    46801 tttaattaat tt
//
LOCUS       DAMJWS010000021        46441 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMJWS010000021 DAMJWS010000000
VERSION     DAMJWS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.021"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            94..849
                     /locus_tag="PC399_001264"
     CDS             94..849
                     /locus_tag="PC399_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2592137.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            980..2002
                     /locus_tag="PC399_001265"
     CDS             980..2002
                     /locus_tag="PC399_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2592138.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2166..2723)
                     /locus_tag="PC399_001266"
     CDS             complement(2166..2723)
                     /locus_tag="PC399_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2592139.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2729..3805)
                     /locus_tag="PC399_001267"
     CDS             complement(2729..3805)
                     /locus_tag="PC399_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF2592140.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3806..4489)
                     /gene="modB"
                     /locus_tag="PC399_001268"
     CDS             complement(3806..4489)
                     /gene="modB"
                     /locus_tag="PC399_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF2592141.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4638..5450)
                     /gene="modA"
                     /locus_tag="PC399_001269"
     CDS             complement(4638..5450)
                     /gene="modA"
                     /locus_tag="PC399_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2592142.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMEDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5643..6572
                     /locus_tag="PC399_001270"
     CDS             5643..6572
                     /locus_tag="PC399_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592143.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6733..7182)
                     /locus_tag="PC399_001271"
     CDS             complement(6733..7182)
                     /locus_tag="PC399_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592144.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7385..8365)
                     /locus_tag="PC399_001272"
     CDS             complement(7385..8365)
                     /locus_tag="PC399_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2592145.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            complement(8570..9430)
                     /locus_tag="PC399_001273"
     CDS             complement(8570..9430)
                     /locus_tag="PC399_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2592146.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(9747..11075)
                     /locus_tag="PC399_001274"
     CDS             complement(9747..11075)
                     /locus_tag="PC399_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2592147.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(11223..11552)
                     /locus_tag="PC399_001275"
     CDS             complement(11223..11552)
                     /locus_tag="PC399_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2592148.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(11576..12865)
                     /locus_tag="PC399_001276"
     CDS             complement(11576..12865)
                     /locus_tag="PC399_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2592149.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(12881..13207)
                     /locus_tag="PC399_001277"
     CDS             complement(12881..13207)
                     /locus_tag="PC399_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2592150.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(13342..13971)
                     /locus_tag="PC399_001278"
     CDS             complement(13342..13971)
                     /locus_tag="PC399_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592151.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(14187..16844)
                     /locus_tag="PC399_001279"
     CDS             complement(14187..16844)
                     /locus_tag="PC399_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2592152.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKHYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(17203..18147)
                     /locus_tag="PC399_001280"
     CDS             complement(17203..18147)
                     /locus_tag="PC399_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592153.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(18140..19165)
                     /locus_tag="PC399_001281"
     CDS             complement(18140..19165)
                     /locus_tag="PC399_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592154.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(19240..20808)
                     /locus_tag="PC399_001282"
     CDS             complement(19240..20808)
                     /locus_tag="PC399_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2592155.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGVFWNIADWTIE"
     gene            complement(20921..21832)
                     /locus_tag="PC399_001283"
     CDS             complement(20921..21832)
                     /locus_tag="PC399_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2592156.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(21816..22802)
                     /locus_tag="PC399_001284"
     CDS             complement(21816..22802)
                     /locus_tag="PC399_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2592157.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            23162..23563
                     /locus_tag="PC399_001285"
     CDS             23162..23563
                     /locus_tag="PC399_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2592158.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            23566..23964
                     /locus_tag="PC399_001286"
     CDS             23566..23964
                     /locus_tag="PC399_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2592159.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(24414..24638)
                     /locus_tag="PC399_001287"
     CDS             complement(24414..24638)
                     /locus_tag="PC399_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2592160.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(24673..26040)
                     /locus_tag="PC399_001288"
     CDS             complement(24673..26040)
                     /locus_tag="PC399_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2592161.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTAMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(26124..27659)
                     /locus_tag="PC399_001289"
     CDS             complement(26124..27659)
                     /locus_tag="PC399_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592162.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDAILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(27682..28116)
                     /locus_tag="PC399_001290"
     CDS             complement(27682..28116)
                     /locus_tag="PC399_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592163.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(28376..29287)
                     /locus_tag="PC399_001291"
     CDS             complement(28376..29287)
                     /locus_tag="PC399_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2592164.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            29583..29936
                     /locus_tag="PC399_001292"
     CDS             29583..29936
                     /locus_tag="PC399_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2592165.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            complement(30155..31096)
                     /locus_tag="PC399_001293"
     CDS             complement(30155..31096)
                     /locus_tag="PC399_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2592166.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(31197..32432)
                     /locus_tag="PC399_001294"
     CDS             complement(31197..32432)
                     /locus_tag="PC399_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2592167.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSQSKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLI"
     gene            complement(32476..33330)
                     /locus_tag="PC399_001295"
     CDS             complement(32476..33330)
                     /locus_tag="PC399_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592168.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(33458..34633)
                     /locus_tag="PC399_001296"
     CDS             complement(33458..34633)
                     /locus_tag="PC399_001296"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2592169.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(34732..35616)
                     /locus_tag="PC399_001297"
     CDS             complement(34732..35616)
                     /locus_tag="PC399_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2592170.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            complement(35848..36534)
                     /locus_tag="PC399_001298"
     CDS             complement(35848..36534)
                     /locus_tag="PC399_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2592171.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            complement(36562..36873)
                     /locus_tag="PC399_001299"
     CDS             complement(36562..36873)
                     /locus_tag="PC399_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2592172.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            complement(37062..37583)
                     /locus_tag="PC399_001300"
     CDS             complement(37062..37583)
                     /locus_tag="PC399_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2592173.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            complement(37680..38174)
                     /locus_tag="PC399_001301"
     CDS             complement(37680..38174)
                     /locus_tag="PC399_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2592174.1"
                     /translation="MIRKANMNDLESIMQIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            complement(38208..38681)
                     /locus_tag="PC399_001302"
     CDS             complement(38208..38681)
                     /locus_tag="PC399_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2592175.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            complement(39007..40002)
                     /locus_tag="PC399_001303"
     CDS             complement(39007..40002)
                     /locus_tag="PC399_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2592176.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            complement(40024..41949)
                     /locus_tag="PC399_001304"
     CDS             complement(40024..41949)
                     /locus_tag="PC399_001304"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2592177.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREEAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            complement(41974..43167)
                     /gene="nifV"
                     /locus_tag="PC399_001305"
     CDS             complement(41974..43167)
                     /gene="nifV"
                     /locus_tag="PC399_001305"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2592178.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            43629..44738
                     /locus_tag="PC399_001306"
     CDS             43629..44738
                     /locus_tag="PC399_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2592179.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(45115..46299)
                     /locus_tag="PC399_001307"
     CDS             complement(45115..46299)
                     /locus_tag="PC399_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592180.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT        15163 a         8203 c         4977 g        18098 t
ORIGIN      
        1 ctttcctagc tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta
       61 ccaaaaataa atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag
      121 agcttcccta atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata
      181 gaagatggtg aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta
      241 aatataatat ccgggcttat aaaagaatct tctggtcaag tattattaaa taaacataac
      301 ttgtctaatt tagctgaaca cagaagaact caaatagtaa gtagagtttt tcaagaccca
      361 agtcttggaa cttgcccttc tatgactgtt agagaaaact tgtctttagc tcttaacaaa
      421 ggaaaactat taaatctaag aaaatgtctt cgttacaaaa gagatttctt agaaaattta
      481 ttggaaggtg tatctcttga tttaaaaaaa taccttgatg ttcaagtaaa atttttatca
      541 ggaggtcaaa gacaatcatt atcactaatt atgtcgtgct taacaaatcc atcagtttta
      601 ttacttgatg aacatacagc agcacttgac ccaaaaacat caaatgaagt aattgaattg
      661 actaataaga ttgtaaggga aaaaaatata actacactta tggttactca caacttaaaa
      721 cacgctcttc attatggtga tagattgata atgcttcata agggtgaagt tgtattagat
      781 gtaaaaggta aagaaaaaga acaattaaca gttgaagaaa tcttagaaaa atttgaatat
      841 gcagtttaaa ataatatatc tattgtatta aactcggctt taaaataaag ttaaaattac
      901 aataattatg gttactttgt aataattcat atgaatatat gaacatatat aataataaat
      961 ttttgagggg gatataaaaa tgattaataa aaaaagattg gcaagtttga ttttagctgg
     1021 tgctcttagt atatctatgc ttacagggtg ttcacaagga ggagactctg gtaattcaaa
     1081 acaagaaagt aattctaaag gtaaagaagt taaaaaaatt ggtataactc aactggttga
     1141 acatccagca ttagatgcca ctaggacagg ttttgtaaaa gcattagaaa agaacggatt
     1201 taaagatgga gaaaatattg atatagactt ccaaaatgct caaaatgaca tgcctactac
     1261 acaaagtatt gcgagtaaat ttgcatctga taagaaggac ttaatatttg ctatatctac
     1321 tccatcagct caggcagcat ttaatgctac taaagatata cctatactta ttactgctgt
     1381 gagtgaccct gttgctgctg gattagttaa aactcttgaa aagccaggca caaatgtatc
     1441 tggaacatca gattttgttt ctgtagacaa aggtttagaa cttcttaaaa tatttgctcc
     1501 taaggcaaaa actataggag ttatgtacaa tactagtgaa gtaaattcca aagtccaagt
     1561 tgatgcttta aaagaatatg catctaaaaa tggatttaaa gttgttgaaa aaggtataac
     1621 tacttcaaat gaagttaacc aaggtatttc tagtttagtt ggcaaaatag acgtattata
     1681 tgttcctaca gataacttag tggcttcttc tatgcctata gtctctaaaa tagctactga
     1741 gaataaaata cctgttatag cagctgaatc tgggccagtt gaaaaaggtg ctcttgcttg
     1801 tcaaggtatc aactacgaaa aactaggtta caaaacaggt gaaatggcag ttaaaatttt
     1861 aaatggtgaa tcagtatctg atatgcctgt ggctacatca gatgatacag atatcattgt
     1921 taatgaagat atattaaaag ctttaggtat ggaaaaacct tctaatgaaa atatttctta
     1981 tgtaaagact aaacaagaat agaaagatat tttctatata cgattaatat gcctgtttct
     2041 ataaattaat ttatagaaac aggcatatat ttttatttta atgtattatc tatttaaaga
     2101 ttatatgtaa atgctcttaa tttgtttgta taaattttac ttgtataaac tttatacaat
     2161 catatttatt cttctatttt ctttattact ctacatggat ttccaacagc tacagtattt
     2221 ggtggtatat cttttgtgac tacacttcct gcacctatta ctacattatc acctatagta
     2281 attcctggtg ttatgattac tcccccacct atccatacat tatcacctat ttttacagga
     2341 gaaccatact ctatacctga atttctaagt tgtgcatcaa ttggatgata ggctgtatag
     2401 atttgaacgt ttggagctaa cattacattg tcacctattt caattttaca tacatctaaa
     2461 aaaatacaat cataatttgc aaagaaattt tcgcctacat gtatgttata tccataatca
     2521 cacctaatat tttgttctac atttatttgc ttgccaactg aaccaaatag ttgtcttaaa
     2581 atatcttctc ttttttcata ttcatcctct aaagtattat taaacaatct agttaatttt
     2641 ttacaatact ctctttcttt tactaacaat tcatcatttg catagtatcc tttcccagac
     2701 aacatttttt ctttttcagt catatagttt actccttttt gttataattg tatctaaagc
     2761 aaaataaata ttcctgtgga aaatctaaat atattctgtc acctttctta aatctcattt
     2821 tacttttttc caattctatt tgaataggta cacattcgtt agataaatca gtttttctaa
     2881 catatatagt atatgtaaaa ggattttcta caatgttaat caattcgaaa cataactttt
     2941 catttaaatc ttcattagaa tccaagacct taatatgatg ctccctgatt cccacatatt
     3001 ctatattatc acctatttct ctatttaatg tcaactcgca tccccagtct gtagcataaa
     3061 tagtattttt atccagtcta tttaatttgg atatattttt acaccctgtt atcttagctt
     3121 ctataaggta ctttggagat tcaaagagct cttttttagg tctctttgga agaccttctc
     3181 ctttgttgta cactataata tcatcacaaa ctcggtaagc ttcttcaata tcatgagtaa
     3241 caaatagtat atctcccttg taatctttta acatattcat aagttctttt tccatattgc
     3301 ttcttaaatg atggtccaat gctgagaaag gttcatctaa aagtaatata tctggagatg
     3361 ttgccaatgc tctagctagt gctacccttt gttgttgacc accagataat tgccaagggt
     3421 atctcccctc aaacccattt aatttaagta tatctaagta ccttgctact atttcctttt
     3481 tttcactctt acttagtttt aaaagcccaa gttctatatt ttgacttaca gtcatatgtg
     3541 gaaacaaagc atagttttga aataaaaatc ctacctttct atcttgtgtt gataaattta
     3601 tcttcttttc agaatcaaat agaactctat catttaatac aattttacct cttgttggtt
     3661 tctcaagccc tgctatacat tttaatgtca tactttttcc agaaccagat tctcccaaaa
     3721 accccagagt acctttttct tgtttaattt ctacctttag tttaaaagag gataaatctt
     3781 tttcaatatc cacgtataag ctcaactatc cacacctctt tcctataatt ttctgctgaa
     3841 attccgacca ataatttagt aataaaatca ttgctataga tattgctaca ataatcataa
     3901 cccacatcat agctttattc atatctccgc cttcaacagc aaaaaatata gctattggca
     3961 tagtttgagt ttttccaggt atatttccag ctatcataag tgtagcacca aattctccaa
     4021 gagctctggc aaacgacagc accaatccac caattattcc aggccatgct aatggaactg
     4081 caatcttaaa aaatactttc cattcattaa gacctaatgt tctagcagct gacaacatat
     4141 tattatcaat ctgttcaaaa gctgaccttg aagttctata catcatagga aaagacacca
     4201 ctactgctga aattacagtt gctgtccaac taaatattaa agtcctatcg aaatttaata
     4261 aaaactttcc tataaatcca ttcttcccac atagtaataa taaaaagaaa cctacaactg
     4321 ttggtggcaa aattagtgga agtgtaaata gtccatctat aagacccttc cacttacctc
     4381 tatagttagc cataatatat gacacaaata ttcctattac aaaagttatt attgttgaca
     4441 aaatagatgt cttaagtgat atccataagg gtgaccaatc tgttcccatc tttccctcct
     4501 taattttaaa tggaaatagt gaaactaata actctattaa tttcactatt tccattttat
     4561 tataatacac aattattttc aattttcatt tatttatata taatttatac ttaattacac
     4621 ataatttatt tctaagttta ttctactttt ttatatccaa attcttcaaa aatcttttgt
     4681 cctgactcac ttaataaaaa ttcttcaaat ttcttagcag catctacgtt tttactagct
     4741 ttaattactg aaactggata tgtaataggc gaatgagttt tttcgtctgt tttttcaact
     4801 acttttatct tatcattatt gacagtatca ctgaagtaaa cgaatccaac atcagcattt
     4861 cctgattgca cccatgctaa aacttctttt acatcttttg caaatactag cttatctttt
     4921 aacttatctt ttaaatttaa attagttaac acctcatctg catacttacc agctggaaca
     4981 ctttcagctt caccaacagc aattttcttc actttatctg tagtcaaatc ttccatacct
     5041 gatacactac tgtcagcaga agaaataaga actaaatcat tttttactaa atctttcata
     5101 gtatcagaaa ctaataactt ctcttcatcc aaagctttca tttgtttttg acctgctgaa
     5161 ataaataaat cacaaggtgc tccttgttct atttgttgtt gtaatgagcc tgatgctcca
     5221 tagttaactg taagttttac atttgaatca acttttttat attcttcttc aatctttgcc
     5281 attgcttctt ttaaacttgc agcagccgat atatttaact ctactgaatc actactttta
     5341 gttgtttttt cttgttttcc atcatctttt ttgtcttcat tactattgca cccaactgtc
     5401 cccaaagtta aacatgcaac taatcctaat attcccaata tttttttcat aacaatctcc
     5461 ctttttgtaa atttgataca cattttaaca tgttttatta tgttttatca tgttttgtta
     5521 tatatagttt aattatatat acatgataat aaattttcaa gtaatttatt tacactactt
     5581 ttaaatattt tgctacattt tccttttttt aatgttaaaa taaccttata tggaggtgga
     5641 ttatggactc attatcatct ttgacacctg tcgaggtagc tgaattatta aagataacaa
     5701 aaaatactgt atatgaatta atcaaaagag gtgaacttcc ttcttataaa gtagggaaaa
     5761 aaataagagt tgatactaaa gatgttgaag aatatatcaa cagccaaaaa actggtaaag
     5821 taaaggatac acatttcaac aataaaaata tagatataaa taataatttc aaaattatta
     5881 tttctggtca agatgtaata ttagacattt tagctaggtc tgttgaaaaa aaattggatg
     5941 gaataagtac atttcgctct tatataggta gttataatgc tttgtatgag ttatataatg
     6001 gcagagtttc tatagcatca tcccatctat gggatttcga aacggatgaa tataatagca
     6061 cttttgttaa aaagctattg ccaggaatac catgtgtact tataaatcta gcttatagaa
     6121 tgcaaggttt ctatgttgct aaaggaaatc ctaaagagat aacaacctgg gaggatttaa
     6181 caaaaccaag tataaccatt gtaaataggg aaaaaggttc tggaactagg gttttactag
     6241 atggtaaact tagacttcta aactttaatg gtaaacatat aaaaggttat gataatgaag
     6301 aattatcaca tcttggagtt gcaagtactg tttctcgtgg aatagcagat gttggtttgg
     6361 gaaatgaaaa agctgcttta caagtaaata atatcgactt tattcctctt cacaaagagc
     6421 gctatgattt agttattaaa aaagaagact tacaaaatcc agtttaccaa actataatta
     6481 acataataaa ctctcctgag tttaaagctg aacttcaagg tcttggcgga tatgatttaa
     6541 ctgatactgg aaaaactata gcaaaagttt aagattaaaa tcgcaatcta ttttataaga
     6601 tttataattt ttcacaaaat ttataactta aacacgaaat aaattgcgat tttagtgtct
     6661 caaaaaattt atgtctttca ttttatcatc caatacaaaa tataatataa ttaattgtgc
     6721 aatcatatta tattaatata tatttacata atagtatttt tctttaatgt agattaacat
     6781 atgatatgtt ttaaacatca atccccttgc tttcttatat atatttggca acacatatga
     6841 gtttaataaa ttatatacaa tgttgctttc ttcttgtatt ttttcaacca acaagttatt
     6901 tataaaatta cctttctcat tttcaataat ctcactccaa tttatcttaa cttttaatat
     6961 atatcttccc actaggtata cacactctac aataaataat aaaaatacaa tgcagtcagt
     7021 acctgttgtc ttatataaat tacccgatac taatggcttt gcgaagaaat tataaaaatt
     7081 caaagtatca cccgtataat caaaaagtat taatagcact cctagaactc tcaatacttt
     7141 aatattcaac atgtttttat ctttattatt tttgtttttc atatatacta cctctctttt
     7201 ctttagattt gattgtaatc taatactaac tcttttattt gaggtataaa tgaatttaat
     7261 tggtagttat tttcattttt actagaaatt aattcacatt actaaacaaa acttatattt
     7321 aaacataaaa atagcctatc tatcttttaa taaatacaaa aataaataag ctactaatat
     7381 tcaattaact tttatcactt aaaacattac tatctataaa tttaaatatt ttattgaagt
     7441 atactgtact atcatgttca aatccatcaa catgaccaat accttctata agccataggt
     7501 cttttttatc atgtttaact aaatcataca ttttcttgct attttgataa ttacaaacag
     7561 tatcttttgt tccatgtata aacagtatgg gtacattaga tttctcaact gactcatatg
     7621 gttttacatc actgtaccaa aatccagatt taaacttatt atataaatct ccacaagtca
     7681 ccacataatt gattaatata tctggtattc gtttttctag tactcccatt ctaattgcat
     7741 ctttcatttc agaataagga gaatccagta tataaaaatc gaccttatca tcttttgaat
     7801 ttatctctga atgcatagca gctgtccctg cccccatgga aaatccatgc acacctaatc
     7861 tcccttcagg aaatttattt tttacaaatt taactagact atctaaatca tacctttcat
     7921 aaagaccaaa tgtgtaatta tctccaccac tattaccagt atttctttga ttataaacta
     7981 atacattata acctttatct aaatatctat atgctacctt taacatttca tagtaactac
     8041 ttccaattcc atgaacaagt atcatagtat ctcttgtctt tatatttgaa gttataaaaa
     8101 gactttcaag cttgtaatta ttagaacttt gtatcattaa ttcagatgtc ttgtagtttt
     8161 ttaatttctc cagtggttta tcttttctct cagaaaatat ttttactata tcttcgtttt
     8221 ttattaactg ctctaaacca actgaactat tatacactaa ttttccaaat atataagata
     8281 caattaatag gattaataga aacacaattg atataattat aaatttattt cttaaactaa
     8341 tacaattttt aaaactattt ttcatattta tttaccatac aattttccta tattatattt
     8401 tctaagtaat agcataattg attatactat tcttataatt tctattatac ctacttattt
     8461 attaaaaata caaattatat tttattaatt tttactactt gtaaattatt atcttataca
     8521 atgaatttgt attatataat aagtattttt aggattctgt tacataatat tatttaaata
     8581 ttcttctata tttctcttct tcttcttcag taaacacatt aaatacaact tttaaatttt
     8641 tttcaggatt ttcttttagc catagtctaa ctctacttac agcaagatta gctgcctctt
     8701 tttttggata accaaataca cctgtagaaa ttccacaaaa aacaatattt tttatttcat
     8761 caatttcttt tattgtattt aaacatgatt tataacaatg aagcagttgc ttttcttgct
     8821 ctttacttgg ctgaccacca gatacaatag gtccaacagt atgtactaca aattttgcag
     8881 gtaaacaata acctctagtt atcttagcat ctccagtata ttctaaatgc ccttgttttt
     8941 ttattatttt gtcacaatct tctctaagtc taggacctgc gcatgaatgt atctcattat
     9001 ccacgcatag atgtaatggt tgtaaacacc ctaataactt gttatttgca gcatttacta
     9061 ttgcatctgc tcttaaattc gttatattac ctctccatat ggcaatccct tcctctactt
     9121 cttctatatc atttacatct actaactctc tttcttcaca ttcaatcaac aataaattat
     9181 gtaacatatt aagtatctct ggagcaattt cttttggtgg atatacattc aaaagtcctc
     9241 taagtatatc tctaggctta atttcatcat ttgataaatt gtatatttta gaaggaactt
     9301 cttttgaaaa atatgcaatt aaagtgttta tattttttct tctttgttcg tcagttagag
     9361 gcttgatagt tttatcaaaa tcctcaaata aatttacatc atcagcataa tctctccact
     9421 tcatagccat ctctccattc ttgtatttta gacttaaaaa taattatgta tttatgtaca
     9481 tatttattta tttctataaa cacctacata aatcctgtta aaagtgtaaa ttttttatta
     9541 tacaacttct ctggttattt ctattgattg atagtaaatt attgtaccta aatttaatcc
     9601 aactaacaag taactaaacc tactttccta actgcaaaat acactatgtt tgccacttgt
     9661 atctcatttg tagataaata aagtccctac aaatacatta actaggctat gtactaaact
     9721 gtaggaactt taaaaatata ttattcttaa ttaagttttg gccaataatt tttatttgct
     9781 tctattaaat catctagtaa ttcttttgca actgttgcac taggaactgt cttagatagc
     9841 ataagtgctt gccacaattt attataagac ttctcaatcc aagcatcaac tactaatttt
     9901 tcaactgcta attgttgttc tattaaacct ttttggaatg ttggtatttt ccctattgat
     9961 aatggttcat acccgtcttt tccaactata catggaattt caaccatagc atcatcttgt
    10021 atattttcta tagctccatt attttgaact atcaataaca tacgctcctg tgtattaaaa
    10081 gctagagctg tagctaaatc cactataaac tctgcatgta ttcctatctt aagcccactg
    10141 cccttagaat gtccttgctt ttctatcaat gcacatgaac caaaaacttc tttttctctt
    10201 ccatcaataa cttcattggc acgagtataa tttacatcag aatgatttac aacataatct
    10261 ggaaatagat agtactttaa gtaactactt ggtattgtag ttaaatctgt ttttactatt
    10321 tctttagtaa ataaattagt ttcaaaccaa ctatctttct tgtcattttt cattccttct
    10381 tcaccattta taaaaccata ttcttttata tgttctacta attttggtaa aagttcattt
    10441 ccttttttat ctttaattga agtataccaa ccaaagtgat ttaatccaaa gtatcttata
    10501 tccatatgtt tacgagattt gagtcctgca atccttgcta tattatgttc catacctatt
    10561 ggcatatcac aaatatttat taccctagaa ttaggtctca accttctaac agcttccgct
    10621 attattgatg ctggattaga ataatttaac atccaacaat ttggagaata cttttccata
    10681 taatctatgt tttcaagaac accttctata gacctcattc cataagctat tccacctggt
    10741 ccacaagttt cttgaccaac taccccatgt tttagaggta tcttttcatc tagttctctc
    10801 atttcaagtt ttccaactct tatatgagct aaacaaaaat ctacatctgt ataagcctct
    10861 ttagggcatg ttgttgctaa atactctata ttagggtcct tttcctttat tagtatttca
    10921 caagctttag ctaccttatt ttgtctttct tcatcattat catataattt gattgctctt
    10981 aatggtaacc tatctaagtt atctagcaac attaatatga tttctggggt atatgtactt
    11041 cctcctccag ctattactac tgaaaatttt tccataataa tatctccttt tctatttaaa
    11101 aatttactgt atttataaat aaatttataa atactagtac tttaaagtaa aagggagttc
    11161 atttattaaa tctattgatt taataaacta tagccaaact tcaagcacta tttacaagaa
    11221 atttaattct tattaattct tgggtccatc atctcaaata tatctacaag ttcagatact
    11281 aaagaatgag caaataatgc atttgaataa tggtcttcag agtgcacaac taaaaaatca
    11341 gtcccactat cttcactatt catagcattt gatagtaatt ctaaatgatg ctttgatgct
    11401 gtttgtattt tctctgaggc tgaatttaaa aggtgttttg ctttattgta gtcacctttt
    11461 tttatttcct taagagacat cattacatca cttttagcat ctcctgcttc tgatattata
    11521 ctaaatactt tttcttccat ttcaggtacc atatccttct ccttctaatg acttgttaaa
    11581 aatcaaagct atcatcatct aatgttgtag ctaattctgc atttttattt aattcttcct
    11641 tctccattat cttaaagaat ggataatata tcaaggtcat aagtattata cttgcaaata
    11701 ctaatacgat tgccttccaa tctaatgtca tcaaaaatgc tgcaacccct ggaggtaaaa
    11761 agcctggtgg ttctgcaata ggtaatccta ctaatcctac ttttaatgcc ataaaattaa
    11821 gtgctccaac caatacaggt ccaaatacaa atggtacaaa catatatggg ttcataacta
    11881 ttggtgttcc aaatattacc ggctcattta ttccaaatag tgatggtaaa atcacaactt
    11941 ttcccatact attaaaactc ttacacttac ttcttgacat aaggtacact aatcccaaag
    12001 ttattcctga accagttact tgcaagaaac catagtaaaa tcctattgta aacacatatt
    12061 ttgggtctcc tcctgctgct atcagtgttg cattttctgc catatatgta gctgctattg
    12121 gcatccatat acttgaagtt attgctgctc catgaagccc aaagaaccat aataattgag
    12181 ttataaatag tattatagtt actgcaaaaa cattatctac actgcttata gctggtgcta
    12241 agaaattcat tataatagct ggtagtggtt gacctgagac atttttagtt attgctgcta
    12301 ttgctgtaaa taacattata catactatca ttggagttaa atttctaaaa cttttattta
    12361 ctacactagg tacttctttt ggaaatctaa ttacgagatt tttatcattc ataaacttag
    12421 taatatcaac tactaaaagt gctactaata tacttacaaa taaaccttgt gcatctaaat
    12481 aacttgtatc taatacacca tctactactt gtccacatac aacaagttgt gcaacaactg
    12541 ctgtcatgcc tatttctaca tgattaagat tatatttttt agctagtgcc tgagctataa
    12601 aaaatgctga atatatagat aataaactca tagtaaattt attagggata agtaatgtat
    12661 ccatattttc agataataat gtttttgcac ttccttcagg taacatattg tttaatgcca
    12721 taaataacat acttaaagaa ccaacaatta ttattggggt aatgcttatc ataccttctt
    12781 ttatagcttg aagatgcctt tgtttttcaa tcttttgtgc tattggaaga agaaatcttc
    12841 ttaaattatt ttgaagtgct tccattttta ttccccccaa ttatctattt ttatataatt
    12901 cgattgccga cttcataaca gaaggagcat ccatacgacc ataatgctta gtatctatta
    12961 aaccaactgc tattttgtaa ttcttacaag cttcaactac atcatcttcc ttaaacctaa
    13021 tttgtggtgc tacaagtact acatctgtat cattagcttc aatgtatgat tttaaatttt
    13081 caaatgatgt tgcctctata tttatagttt tagtttttaa cttttcatgc tcactacaaa
    13141 aacttttcat cttttccaca agtacacctg ttgaagctcc atttgcacat actaataaaa
    13201 tattcataat taccctccta caatatctaa cctcttttat tttctaattt tttatcagcc
    13261 aattcttact ctaattccta tcagttttta ctttaattct tattatttta ttttaattct
    13321 tattaatatt tactctaatt cttagtactg ccaatgatta aacttgaaag ccgtatctat
    13381 aacttcttct tgcttatatt tttctaatat ttttaagtat ctttctttat aatcattatc
    13441 tacttctttt tctggtatta ctatactagc ttcatttaaa aagtggaatg aatcccttcc
    13501 catttctaat cctctatatg tatgcttgtc aagtgccacg tccataactt ctggaacttt
    13561 acccattgca aatcctttta taattatatt ttttaataaa tcacttgatc tatctttctc
    13621 acatgcacaa agatatctta ttgcatgtat gaaaaataaa ggtctgtctc catctgcata
    13681 tgaatattcc tttcttactt gatttaaatt atttatgatt attacagctg atggatcccc
    13741 cattcctata tcttcaactg atattgtaag tatccttctc catagcttat cttctaattc
    13801 tggtgatgat atatatagtt cataagcaaa ttcacaagct tcttccacca tatttcttct
    13861 tatagctttt tgaaatgacg atattatttc gtctgcacta aatccgtttc ttgtagtcat
    13921 tcttgcccat gggtcatttg cctttttatc tcttaatatt ttatcttgca tattatttac
    13981 tcctttttat ttaaatttta attatttata ttactcattt aataatataa gcaagtatta
    14041 tgccagtttt tttagacact ttaaaacact gttatttcaa tcatttttat catcaaaact
    14101 tagatacttt gtctaagtat atttatatgt gtctaatatt aatattattt tagactaaaa
    14161 aaaaattacc aaataagttt ttatactcat tggtaatctt tttcgattat ttctgcatat
    14221 tctaatatta ataatatttg atagcactct ttttcattaa tttctatatt atatttttta
    14281 tctaattgag atactatatc ttttattttg cttagactct tgtgataatc aagtgttttt
    14341 tctttatcaa gcttttcaat ttctctagta tttccaatca acccatctac catgaaagct
    14401 aaatgcatca taagtccaat tactttatcg tatttctttt ctatattaaa tactaaaaac
    14461 aattcatcag acacatattt tatatcttta aatattctat tataattaga gctttttaag
    14521 gatgtcttta aaccttcaac tacatcatca taacctatac ttatctttat aatatttttt
    14581 aatttattga tattttcatc cttatctata tcttttttgc taatatagtc aattcctcta
    14641 atattaattc tttgttcatt gattactgca acaatattac tactttcttt aagtttcgta
    14701 atatttaaac ttaaatcttt ttctgtttta tatgggacag atataatatc tattccatct
    14761 aagtacctac tcatcttttc ttctaatatt tttcttgttt tattatctac accttctgaa
    14821 acatgtgatg ctataataat tgttttagac agattttctt tattttgaat ttcattagtt
    14881 ccaaaatagt gctttctcgt gttagtgata gagttgtata cgtcatttaa aggtaatccc
    14941 atattggcct tctgaactgc ttctaacaca gttaatgtcg tcaccatagg aattactctt
    15001 actccacact ttttttcttt ctttataatt tcatcaaagg agtttaatga acccatatct
    15061 gtaaatagta atacatcttt attgtctata ttattaatta aatttttaaa atctacaata
    15121 acttcatcat agcttttatc taacttcata tcgaaagaaa tagcactgca ctttccaata
    15181 atatcaccaa ctgcttttac catactactt gctgtagttt ctccatgcat agcaactata
    15241 atattaatgt tattcactat tttattttgt tcatcttgtt tagcttgtac tatgaataat
    15301 gtaatatatg ctacagtttc tatagataaa aataaatcaa attcattttc aattattcca
    15361 actattttta atgctgtttt aaattcactt ctgttatttc ttctaatttg attcaaatct
    15421 atgttgtttg tagagctact tttatccaat acgcttttta tataaattgc cattgactta
    15481 aatattgatt cacttatgtc tatttcttca tcttctttaa taatttgtct tattgattca
    15541 cacatgcttt taaacttaac attattaaat agagaagcat ctgctatatt catttctaca
    15601 gatttattaa gattagtatt tactgcatta ataatattta tatcattgta gttaataact
    15661 tcttcttctc ctaatgacgt aaatacaaaa tattctattc catgtgttat aacttcatct
    15721 gctttaatag acttctctct ataatcatca ctcacttcat ttatagattt atcaaaatat
    15781 tttttctcta tataaactgg ttctttatta tctcttttat atcttagata tccatttgca
    15841 acagctaatt ttatatcatt ttgaagttga cctacattat ttttacaatc ataaagtaat
    15901 agtgatttaa ttatttcttt atgtactttt attgaaatag aaatactttt tgattcttcc
    15961 ttgaaaaaat ttttgactaa ttccattctt tcaatcattg tcctatctct caatgatggt
    16021 atttctattt tcattggtat tcttctattg aatgtatcta atagtgttga tgtcggattt
    16081 tctgtagtag cacatattat taatacctta gactctcttt ctttcgttgc atctcctagt
    16141 cttttaaatg ttcccttatc tataaaagta aacaacattt cttgaccttc tgctggtagt
    16201 ctatgaattt catctaaaaa tagtatccct ccatttgcaa tttccaaaag accttttctg
    16261 gaagattctg cacctgtata agctccttgt tctaccccaa aaatatgtcc cataagtaat
    16321 tgtgcattat tagcataatc agcacaatta aatgttacaa aaggagcttt attttttcta
    16381 attccactat ctatagaata atcgtacata tatctagcaa tcatactctt tcctactcct
    16441 gtttctccat agataatagt atgaagtcca tttgtaggat agagcattgc gaccttgcat
    16501 ttttctatta taggtcttaa actatgttct ttacctatta gtgtttcaaa actatctttt
    16561 tttattttgt tttctacttt tgttcctttt tttatgccaa gcaactcact ttgtgtatca
    16621 agcaattcat tcttgttaag gttaagattt gcagggatat aatacacagg tcttcctttt
    16681 atcttcgtaa ccataccctc attgaataat tggtttaaat atgtacttat attgtttctt
    16741 gcaattcctg tcttttctga taattggtta gctgtaatcc ctttttcatt ttccctatat
    16801 gatgattcta aaactttcat tactttatct tttttatata tcattacaag ctcccctaca
    16861 ttatagtatt ttattaacta aaacccctat acacacttta ttttttaata aactcttatt
    16921 ctttaactaa ggttataatt tatcaaactt tccttacttt aaattataga aaattattgt
    16981 agaaaaaata agattattgc tcattttttc tacattacta tttttaattt tatagtactt
    17041 tcttaacaac atactttact tgtttttaat tttattatat ttgaatttat tatatttgaa
    17101 aagtagaaat aaaaaaccct attttggagt agttttaaaa tttctccaaa aatagggttt
    17161 tagtttgtat ttcaaataaa tacctgttca agtattttta tactaaacaa cttttaaatt
    17221 ttgttgatat ttatttattt ctgatttaga agaatataca aaatgtccat ctgaaacctc
    17281 aatccaactt aatgactttt catttgaaca atcatatcca ttagaattat actctattct
    17341 atttcttgac ttagcatatc tagggtcagg aacaggaatt gcagaaagta gtgattttgt
    17401 atatggatga ataggattat tatacaattc atttgaatct gccaattcta ctaattttcc
    17461 attatacata actcctatac ggtcactaat atgtctcacc atagataaat catgagctat
    17521 aaataaacaa gttaaattct tttcttgttg caatttttta aataaattta ctatttgtgc
    17581 ttgtatagac acatccaaag aagctatagg ctcatcagct attataaatt ctgggtctac
    17641 tgaaagagct cgtgctatgc caatacgctg tctttgtcca cctgaaaaat cagatggata
    17701 cttatttgca taacttctat caagtcctac tagctttagc atctcatata ctttattcat
    17761 tctatctgtt ttatttttac agataccttg aatttttagt ggctctgaaa ttatatctgc
    17821 aattgtcata cgtggattta atgatgaagt agaatcttga aaaataatct gcatactttt
    17881 gtttacatct ttttttataa atttgtacgt ttttttatct gatatgcaat ttcctttgta
    17941 aattacttct ccaccagtag attcatgtag tcttattata gttttaccag ttgtagattt
    18001 tcctgaaccc gactctccta ccaatccaaa tatctcaccc ttatatatat caaaacttat
    18061 atcatcaact gcttttacag tagtactttt gtccaaatga aaatattgct ttaagttttt
    18121 tatttccagt attttttctc tattcattag aaatcaccct tccacaattt actctaactg
    18181 gtacatctac ttcaggtgca tctgggtgca ataaccaagt tgctgcacta tgtgtatcat
    18241 taattttaaa cattggtggc tctttttcat aatctatctt aagtgcattt ttattcctta
    18301 ttgcaaaagc atctcctttt ggtgggttca ataaatttgg aggcattcca ggtatattat
    18361 ataaatagtc atcctgcgaa tctaaagtag gtaatgaacc caacaacccc caagtatatg
    18421 gatgtctagg gtcataaaat atatcctcta cagttcctat ttctacaatc tttcctgcat
    18481 acataactgc tattctatca gctatagttg caactactcc taaatcatga gttataaata
    18541 ttattgacaa acctatttta tgttgtaaat cctttattaa atcaattatt tgagcttgta
    18601 tagttacatc taaagcagtt gtaggttcat cagcaatcaa tacatctgga ttacaagcaa
    18661 gtgctatagc tattactata cgttgtctca ttccacctga aaaatgatgt ggaaattgtt
    18721 taaatctttt ctcaggatta tcaattccaa ccaattcaat aagctctatg gctctctttt
    18781 ttgcctcact tttacttata ccttgatgaa tgattacagc ttctgcaatt tgctttccta
    18841 ttgatatagt tggattgaga gatgtcatag ggtcttgaaa tatcattgat atattctttc
    18901 ctcttatatc ttccatctct ttttcagact tcttaaccaa atcacttgat ttaagtaaaa
    18961 cctctccatt tttaatatat ccattgtatg ggagtatcct catcaaactc ttacacagta
    19021 cagatttccc acaaccagat tctccaacta tagccaatgt ctcacctttt tttagattaa
    19081 aagatacatt tcttacagcc tgaacttctc cttcttctac tttaaaactt acagatagat
    19141 tattaacctc caataaatgt tccaaaaacc atcaacccct atgctattca aaccttaggg
    19201 atatattttt atccctaagg tttctaatta atttcagtat tattcaattg tccagtcagc
    19261 tatgttccag aatacaccta caccatgatg acctaaaact gtatcaggtg ttaatccttt
    19321 tatgtttggt tttccaacat atattgcatc tatataagca ataaatgtat aaggcatatc
    19381 cttagtcatt tctacttgga attgtttata taactttaat ttttcatctt tatcttctgt
    19441 ttctcttgct ttttgaagta ttttatctat agtaggatta gagtaagcac tgtaatttgc
    19501 acctttatct gttccaaata ctttatatgt atggtcatct gggtcaaatg gacttcccca
    19561 acctattaaa tgagcatctt gattagccca atctgtttca gttacaacaa cagctttagc
    19621 atcaacacca atttctttta actgttgtgc acatatttta gccatatcaa ctctaacttg
    19681 gtcactctca cctgctgtta tctcgaaagc taacttagta ccttcttttt catagattcc
    19741 atcagaacct aatttccaac ctagcttttc aatttcttgt ttagcttttt ctggattata
    19801 ttcaaacttt tctatatcag gattattata tggtcccatt tgtaaaggtg aatatgctgg
    19861 tacaccatgt cctaataata cactgtcaac tattgctttt ctatctattg catagcttaa
    19921 agcatttggt aatcctttag cttttttatc tttaaagaat ttagaattaa aattgtataa
    19981 tatacctcta tagtcggctg ttttcattat atttacttta aaattctttt cgtctttttc
    20041 aaagttagac atatctttag gagttatttg agctaggtct aattctcctg attttaattg
    20101 cattgcctta gccttatcat ctggaacaat cttaaagact actttatcta atcctggttc
    20161 ttttacaaaa taatcactat tcttaacaag tgttatactt tgacctttgt cccatttttc
    20221 aagtttaaat ggtccagtac ctattggttt ttgattaaat tcatcagtag ctatatcctt
    20281 accctccaaa gcatgttttg gtaatactcc tactgttaaa taatcaagca ttgctgtatt
    20341 tggagctttt aaagtaattt ttatagtatt atcattaacc acatcaattt ttgttatatc
    20401 ttcataattt gatgctatct cagaagcatt atctggattc attatagttt ctattgtaaa
    20461 cttaacatca tttgctgtaa atttctctcc atcatgccac ttaacatcat ctcttaaatt
    20521 aaaagtatat gtatttgttg cctcatcaaa tttccagtcc tttgctagac aaggaaccac
    20581 cttattattt tcatcatgag ctgttaatcc attaaatatt aacgaattta tttctccatg
    20641 ttcatatagt gcaggattta tacttgtata gtcattactt ccataaacaa gcacctttcc
    20701 atctttaggc gtatctgcct tcttatcttt gtctcctcca ctagaacaac ctgccattag
    20761 actaagtatc attactaaac ttaaaacttt taacttcttt aatttcatga tgctccccct
    20821 tttgcttttt ataaaatatt tttcaaagct ttgaccccat ttatttaaat agatatttca
    20881 aatacctcag tatttgctta tttaatactc aaatgctgat ttataaatta ctagattttt
    20941 tattattatt ctttcttata taattaccta tatttgttat acacaccaat gtggtaacca
    21001 aaaatatacc cggaatcagt ataatccacc atctatttga aagtaatgct tcctctgaca
    21061 aagataacat acttccccaa gaaacaattt ctattggtaa accaattcct aaaaagctaa
    21121 gtgtcgattc agtaccaata gctgcgccta tgtttgttac aaccatgaac attattgatg
    21181 ccacaaaatt tggaagtaaa tgttgcttta atacatagaa gaatcctcct cccatgctct
    21241 tagctgctag tatgtattcg ctatttttta tttgtctaac ttctgttctc actatcttag
    21301 atatattcat ccaacttgtt atccctatta ctatggacat agatactgga tttgaatttc
    21361 caagtatagc ttgtacaaat attataatta atattgaagg tatactaagt atgatttctg
    21421 taaacctcat cattgcatca tctacgagtt cagatgccga accacttata ctaccataaa
    21481 caattcctat aacagttgat attacagtgg aaaatagtcc tataaataaa gatatttttc
    21541 ctccatacca tatcatagaa tatatatctc tacccattga gtctgttcca aataaaaaag
    21601 ttttatttgg agctaaatta gaacttgcta aatccatgta tgttggttca tgtgtcatta
    21661 taaaagatga gaatacactt ccaatgacta tgattgataa aataataatt gatatatatg
    21721 gtaattcttt aattttttta gataaggttt tctttgtttc tatatgttca tcttctgcta
    21781 ctaaaatata attttctcct acaatttcaa aatcactata ttgtggcttc attagtatca
    21841 cctctatcat atttcattct agggtctatt ttattgttta taatttgagc taacatattc
    21901 ccaaatacta ctaaagcccc tgttattaag cataaaacta ataacatatt ataatcatga
    21961 tactttgcac tttcaaaaca aagtgttcct aacccaggat aagaaaaaac tttttcaact
    22021 acataagttc ctcctagtat atgaggtatc gaaatggcca ttatactaat atatgatggc
    22081 attatatttc tcaggcaatg cttaaaaaca atagttctat tatttaaacc ttttgcttta
    22141 cataaaagca cataatcttc tcttatttcc tctagtaatt tatttcttat catatatgtg
    22201 taataccata gatggcttaa tataagtact gtaagaggta agattagatg tgatatccta
    22261 cttaatatac tggactcttc tcccattgaa taagcaccac tacttggaag tatacttaag
    22321 tttatactaa atataagtat gagtactaag gcaacccaaa atgatggaat acagttggtt
    22381 attgttccta atttgcatat gattctatca atgagcttat cttcatgtaa tgtacaaaat
    22441 attcctaaca ataaggctag cacaaaggta agtatatatc ctgaaccacc taatattata
    22501 gtgtttatat agacatcatt tataacagaa gtaacatttt gtttatattt aaatgaaatt
    22561 ccaaattccc cttttgatgc atttgaaatc cattttatat attgactgta tattggttca
    22621 tccaaaccaa gttttttcat agcattttct ttttcttgtg tactcattct ttctactcca
    22681 tccccatagt aagagattaa tgggtctcct ggagcaagtc ttgccatgta aaaaacaata
    22741 aaagataata aaaacattac taaaataaac tgcaacaact ttttcagtat atcctttaac
    22801 aaaccccaac ccccctttgt tgcaataaaa aaaaccacat aaaagcaaac gtaaagtaac
    22861 gttaaaacct tcgtggttcc attatcatta aacaaaatta aattttctat atttatctat
    22921 gagttaattt tagcatcatt tattaactgt gtaaacaaac tttttaaaaa ttgttaaaat
    22981 ttcatcaaat tattttattt atacctaaaa taaaattttt atattctatt cttcattatt
    23041 ttctataaaa ttataaaatt ttaggttgac tatataagaa aagtatatta tattagtttg
    23101 tatgcaaacc atttaagagg tgattttatt gaaaagtaat tatgactgtt ttagaatagc
    23161 catgctctta aaagaacttt attctaaaac tatgtacaca gtagaagaaa attttaaaga
    23221 aaatggatta acacaccaac agattatagt tataaaactg atagctcata atcaagaact
    23281 tacaatatca caactatgtg atgaaatgtc tttagctaaa ggtacggttt caggcataat
    23341 cagcagatta gaacagattg gatatataga aaaattcaag aaatctaatg ataaaagaaa
    23401 tacatacgtt aagtttacaa caacaggatt tgaatttgcc acaaatttta aaattaaaat
    23461 gcaagaaagt tttgatgata tttttaaaaa ttgtgatgaa aacgaattga gtgacttagt
    23521 aaaaaatctt agaaatatat tggcaaaagt aaaggagaga taattatgaa ttataaatta
    23581 atacttgcaa tagtatttat aacatcagct ttaattttct acactatagg tgtatttgga
    23641 gaaagaaagg caaaaatttt aaaaaagaaa catgtaatta tattttggct tggttttata
    23701 tttgatactt taggaacatt tacaatgagt aacatagcca acagccatac ttttgaagta
    23761 aaatctgctt tatcacaaaa ccttcatagt attacaggtc ttcttgcaat tgtgcttatg
    23821 ctttttcatg caagttgggc tacttttgtc ctatacaaag atgatgagga aaagaaaaaa
    23881 ttctttcata agtttagtat agttgtatgg actatatggt tagttccata ttttataggt
    23941 atgtttattg gtatggctgg atagtaaaat aaaaagtgtg taagaaatac tgtaggtaaa
    24001 tttgtataag acaaatttat ctacatcctt ctttcacact tttacatatt tatttataat
    24061 actccattat ttctttcatg tttcttaata cctatataga atatttaatt atttctatag
    24121 tattttatta ttttctcaca ctttttcata tatatattgt tttactattt cttcatatgt
    24181 atatatgaaa tattccatta tttctataaa tgttttatta tctttctcat actttttcat
    24241 atatatattg cttcactatt ttttcttatt tcttcatata catatgaaat atttcattat
    24301 ttctataatg ttttattatc tttctcatac tttttcatat atattgcttc actatttttt
    24361 ttacttcttt attaaatact tatgtataac atacatttaa taaacgctaa ctattaaaca
    24421 cctaatactt gagttactcc tggaacttca ctcaccaaaa cattttcaat aatagctttg
    24481 attgtcatag tcgctcctgg acacccacta catgcccctt gtagtctaac taaaaccaca
    24541 ccattttcat ttacatctat aagctctaca tctccaccat ctctttgtaa tactggtttt
    24601 atcttttcct caagcacttt ttcaactttt tctctcatag ctattccccc cagaaatatt
    24661 ttattagcaa aattatacca atggtttaaa tttttgtcca tctaatgatt tatcaaattc
    24721 aatttttgct tcatcaataa ttttagtatc cataaataca tcataagcac tacaagccag
    24781 tactttagct gaatttatca tagcagaaaa tcctatattt gaacctgctg atgaacatga
    24841 ctgccatgta tgacttgcaa ttccaacagg ccaagctgcc attgcaaatt gagccactgg
    24901 tattacataa cttacatctc caacatcagt agaaccagct agtcctttat ctttgtcacc
    24961 gtcaactatt tccttgtgta tatagctttc tacaatagat ttatcatttt gtaatgatgt
    25021 tgctacacct aatctttctt ctctagtaag agtatcacac aacttttttg caaattcttc
    25081 ttcttcagga gtatttttag ggcaaccaac aaaaaccata ttgtcataaa ctaaatcagt
    25141 caaacattgg tttggtatat agtcatatat tccatctgta accttatact ccatttcagt
    25201 ttctgtcatc attgctgctc cttgagctac ttttattaat ctatcaagaa cgtgttctgc
    25261 atcctttgct tttttacctc taataaagta ccaactttca gcaaatccag gaactacatt
    25321 aggtcttcca ccaccattag ttataacata gtgcattctt atagagtcaa taacatgttc
    25381 tctcaaataa ttagctccaa cattcataat ttcaacagca tcaagtgcgc ttctaccatt
    25441 atgaggagct tgagctgcat gggctgttat ccctttaaat ttaaatttta cagataagtt
    25501 tgccagactt ccacctctcc aaggtgcatt tatatcaaaa ggatgccatg taaatgcaca
    25561 atctacatca tcaaaacagc catttataca catagctgtc tttcctccac cttcttcttc
    25621 agcaggacaa ccaaaatatt ttattgttcc attaaattta ttttcttcta ttaatttttt
    25681 tatagaaaca actgctccta cacctgctac acccaacagg ttgtgcccac atccatgtcc
    25741 agcttttcct tctaccaatg gctttctttc tgcacttaca cattgtgata gctctggtaa
    25801 agcatcatat tctccaagta tgccaatcac aggtttaccc tctccaaaac ttgctataaa
    25861 tccagtaggt aaattttcta cttcttcaat tttaaatcct tttgacttta ggtattcttt
    25921 ttgaatgcta cttgcatact tttcttcaaa ggctaattct ggattttccc atattttctt
    25981 agataaatca attaattctt ctcttatttc ttcaacatta tttatgatat actctttcat
    26041 aattttcctc caaatatatg tatttttgat atgctttaca gattatatta tatactaaca
    26101 ttctatttat tcacaatttt ttgttaatta attttattca tatgctcttc tatggagtat
    26161 atcttatctt cactttgcct cttcataaat ttaatgtact ctatcttcca taaagtagtt
    26221 tcagttttct catttaacat aaattctaat atattaattt ctctcattct aatatcttca
    26281 taaaatacta cactacccaa tattgccaat tctaattcac ttaatgatgc aaacaacatt
    26341 aaatactctt ctccaaataa acattcagat tcaagaatat cgataaagtc ttttttagca
    26401 ttgatatttt cctttattat tcttattttt tcaattatag atgtactatc taatatctca
    26461 ttgaatatct ctctaaacga ttcatcatcc aatttacttt catcatcaac aatataatct
    26521 tctaagtgtt cctttttatt tttactcaca gttaccatac ttttaaaagt gttacttttt
    26581 attgagctta gcacagattc aataaatatc ttttcatatt ttttaatata tcttattaca
    26641 tcttcatctt ctatgcttaa tctttttatt aatacttcaa ttgcttccat aagtagtaaa
    26701 gttatttttt tatttattct aatttctctg ttaatttctt caaaatcatt ataatccaaa
    26761 ttactatcta cttcagtgtt aaaactatca tctacttcaa tcaattcaat tatattactt
    26821 tcttcagagt ctataacttt atcttcagta atattattgt acatattttt aaattgtttt
    26881 tctaaaaatt catactcata ttttgaaatt tctaaattag tcacttcatt tcctaaaata
    26941 gtagaaaaaa tacaattatt tataataagt tcaaataaat ttacaagtat agcatcacaa
    27001 tctattttat aagtcacccc atagtcataa aataattttt ctctatcttt tttcttaaat
    27061 aggttacaaa atctattttc tatatctata ctctcaatat aattcttcac ataataaata
    27121 cccttaacat ccatatcatc tagtgccaat ggataatcta tacttactgg aacgtcctga
    27181 gcttcaaatt ttacaatata acttttaaag aaacttgata actccatcaa ggtatcatta
    27241 tatgctattg aattagtatc caattttcta ctcatcatta gtttataaaa atcttttagc
    27301 ctaactaact cttcctctaa tatttcaatg cctccaaggt acactttttt aacattctta
    27361 gcagtcaact ccacaactct tttttctata tccccaagct tatttatata tgcatcaata
    27421 gcataccata ttgaaatcaa cagtttttcg gcaacttctg tttttactga tgtgctttga
    27481 ccatttgtat attttttaat caaatccata agtattagag atatttcttg ttgtatatta
    27541 tatacttctc tttcatctat cactttacaa tttagacatt ccttaagtag agatatggaa
    27601 tattggttct ttattaaatt attttcgttt atattaatat tacttaaatt atttaacata
    27661 caaaatctct cctatcatat attaaacttc tccaaattta aatttctttg caaattcgtc
    27721 tgcttctcct cttacattgt ccatattgcc tgaaaatttt tcataaaatt cacccagtat
    27781 ttctataatc tcatcatcac atatcttgtc atcagtttca ttttttaaat aataaaaaat
    27841 ttcttgcata tcattaatag cctctaaata attgtccaca tttgtatatt gtgatgtata
    27901 tatattttct attaactgtt ttgtaacttt tatatctaat tctattcttc catacccctt
    27961 taaagtatta tctctcgaat ttattatatc ttttacatca gattcagaaa gagctagacc
    28021 ataatctcca gattctatat tagtttcaag cagttcattt ctttctattt cctttaaatt
    28081 ctgatttata gaactcaaaa taataggtga tatcattttt aaattctcct tttttatttt
    28141 ttccattgac tttttttgtt ttttatgcta tacttaaatt aatttatatt attttaccaa
    28201 ccacttataa tagtagtgtt aaaagtggtt tttgtcaact caaaattact atctcaatta
    28261 aacttttcaa ttttctcaac aaaaaaatac catcatttag atatataact ttctataaca
    28321 acttttaatt cagtagttat ttactaaaat ctaaacaatg atatatttat taattttatt
    28381 tatctttttt ggctctactt ctacttttct ttacatttga gcaatttcta caagaaggtg
    28441 aacagtattg agatttagaa tttgtaggaa tataaatttt accacagtac ttgcatttat
    28501 ttaaagaaga aggttcactt gtaattgcaa aggcatagat agtctcaata gctgttttca
    28561 aagaatcaaa ctcccatgca ataattggtt tatctaattg gcttatagaa aatcctatct
    28621 tttgaggatt aaaccctcca gacataatag taacactttc agttaataga ttagatgtat
    28681 ttttatatac acaaatttga ttaaaatgct tacacatatc tttagcaaaa cttacaatcc
    28741 aatctagttg ttcagtgtag aactttgaaa atactaaatc taaaatcaat ggtctctttc
    28801 caaaaaactt tggagattct tctctctttg atacatctat accccttcta tactttttaa
    28861 aatacacatc ttcatcactt gcaaatggta taaacatgtt tacatattca tctgtactca
    28921 atatattctc ttttgtaata taattctttt caatcatcag tactctttct tctccaacta
    28981 tatctctatt atatacactt gaatatatta aacccaataa tccatacttt tttacaaatt
    29041 caagtacttc tttctttagt ttcatttctt ttgtctcttt tgatatatct tgtaatgcct
    29101 cttgtcctat gcgaattata tctactatta tatcatctgc tacatcaaat gggttataca
    29161 tggtaaacac agcatcttct gttggaacta tatataattc atcttcttca gtaattttat
    29221 attcataatc actataccta atccaatcac ttgcaaaatt ccctattaat tctatttttt
    29281 tattcataat aatcattcac acctttcaat gtaatatata atttcatatt actattacac
    29341 ttttagttta ttccatattt tctacactgt caactattct ttttatattt ttagcatatt
    29401 aaaatttata atagccttta taatggattt ctagagctaa atttcttgtt tattgatgac
    29461 taattcataa ttaaatttat aaaaactatt tacatataaa attaatctct ttataattga
    29521 tatgtaatat atttttacac ttacatatta catatcaatt ataaagagag gtatctaaaa
    29581 tcatggatag aaaacgtgaa ttaaaacaat tatacaaaga aatgaaattt gacacaggtg
    29641 tattcataat aaaaaatgat attactaaaa agatttacct tggaaaaagt aatgacataa
    29701 aaagcaaatt taactctctc aaatttcagc ttggtgctgg ctcatgtatg ataaaagaat
    29761 taaataatga atggaaaaaa tatggtgaaa aagcatttac atttgaagtt ttagaactta
    29821 taaaacatga tgataacaaa acagagaaag attatttaga agaactagat attttagaga
    29881 tggtttggtt agaaaaatta aaggaagata atacttatga aatttattcc atataaaaaa
    29941 tcatataata taagaaaagg agatagtata tcaaaataga catttagttc ataagatact
    30001 tttttatata aaataaagta tcttttcata attttaacaa tgaaaaagag gcttatctcc
    30061 atttgatagc ctcttcccat atattttata ccattataaa atgaacaatc tactaatttt
    30121 caatgtgtat attcatttta ctaataacac tccattactt ctttacagca tccaaacatt
    30181 gctctataat actcttatca cttataccta ttgctgttac tttactagct ccacttaata
    30241 tactcttatc acttttattt gaaactaaaa caacaggagc attttttgca agtggtgatg
    30301 caactaaagc ataaactaaa tcgtctccac ttgttatata gaactctttt gctccattat
    30361 agaacttttc aataacattt ttattagttt gatatctatc aacaccacca attctagttg
    30421 ctctatatct gctcactatg taatcactta atgtagactc tcctccaata gcatatactg
    30481 gaacaggaga aactccaaac catgggtcta agtctgtgtc ttcttctgct gaaggtatat
    30541 cttttgtcaa tattatagga gctccatctc tataagcaac agatgcaaca cttactgcat
    30601 cagcttcatc cttaaatcca tttactaaaa acatcttatc actgttttta attttttcta
    30661 tttcacctgc aattttataa cttgtatcta ctctatctac accatcaatt cttataactt
    30721 gcattcttct cataagtaac attttttcta catctttact tatagaattt tctccaccta
    30781 ttacatatat cttagtaact ctattcagtc tttgaagcgt tgcttctggt atatcatcct
    30841 tattagttaa taaaatagct gcattattta ctcctgcaag accacttgca gcaatcccat
    30901 ctaccattgt agaatctgca ttgataagaa ctgctgttgt aaaatcttgc ttatctgaaa
    30961 taagcccagc tgtttcatat ttatctactc cttgaatatt ttcaatctta tctagtgcgt
    31021 ttattgatgt tggaaaacta acacttaaca gtaatgctga aatacctaac gccaatacct
    31081 ttttactaat cctcataaat tacctcccta aaacaaatat atttagtatt agtataatat
    31141 aaaatcattt aaaaatagtg taaaaatagt tacacttttt tataatttaa ataattttag
    31201 attaaacaca attcatccat taaaataact tcatttattc tatatgctaa gccatcccac
    31261 gttttttttg acatatctac agatgttttc ttatttgagt tattcaataa agattgtatc
    31321 ttagaatcta ttgcattata caaattattt tcaaaatcat ataactcttc atcttttgga
    31381 ataccttctt tctccataaa aggtagagaa acatattcta tctttccaga attattaatc
    31441 tcactaccaa tccactcttt aacaccaaga atatcagtag tgattacatc tgtgccacaa
    31501 gataaagctt ctaaaactac aactggtaat ccctcataaa atgatggtaa cacaaatatt
    31561 tgtgacctgt tgaatagctc agctaaatct ctctgttcta actttcctag gaaattcatt
    31621 ttaaatggag acttacttgc cagtttaact atctcattat atgattcgat atcgcttcca
    31681 gtacctgcaa aattgacttc tattaaatct ttactatatt ttaatttaga tatagctttt
    31741 ataaatggaa tcagtccctt tgatttacat atcttacctg caaatactat ttttatttta
    31801 tcatccttta ttttataatt tttattataa aatatatcat cattataacc actaccaatt
    31861 acaactactt tactttgact tactccaaaa gtctttataa tatcatattt ctgattttca
    31921 tgaagagcaa atattaaatc taatttcctt atatttgcta ttatgtattc tttctctaaa
    31981 tcaatggtat ttaactgtct taaacaagtt ccatgacaaa tagacataac ctttatatct
    32041 tttaccattt ccctaacaaa tgcagttaaa aggtacaaat gatgacatat tataagttct
    32101 ggtttaaaat cattcatagc cttatctata tttactttaa attgatattt caatctattt
    32161 atcatgtcta tatttaaatc tttatatctt gtactttcat atggcataga atcactcata
    32221 cctacaacag gaaaatttaa ctccccacaa ttatacttta ctggataaaa acttacttca
    32281 cttggaaaac aatttacatc atcatttaca tctatacctg caattacagc ttgcttatga
    32341 ccgatttttt cgaatccttt tatcatacca gacaaatata taccactacc tgtactattt
    32401 ggtttttgtg ttattatatg aagaattttc acttttcact tgcctccttt ctaaaattaa
    32461 atagttttat acagtctaat cattatatat atctaaatat aaacaagatg aatcatcttt
    32521 tactttaaat cttggcactt taaccgcttt atagtcatta tcttcaaaat cccttacttt
    32581 attatgtata tatcctatac caaatttttc tgctatacgt atcaattctt cttcttttat
    32641 tagattatat ctatcacatg cacttgtaaa accatcactc gttagcatca attgaaaatc
    32701 tttttttatt tcaatgtatc catatataga attttccaca gcttcctcat caaaatccaa
    32761 tatccaatag ccctcttttg tatttttctt caatctattg cttactattg tatccataac
    32821 actagttcta atctcatcaa atgccatctc ttccaagtta gaaagtttac tcatttcatc
    32881 aaatacagta ttatcaaatt tacaaatact cctatctttt ataactttgg aatctccatt
    32941 tttaataaac aaagcacaat ctcctaataa aaaatactct actttatttt catgaaattt
    33001 tacaacaact atagaactag atggtaaatc taatttttca accttaaacc catttaatct
    33061 tagttcatac tcttttttta cttctattat tccagtatta ataatactct ttaatgattc
    33121 ttctttattt atatttttat ataaatacct attccaccac tgaacatacc accttgcgtc
    33181 actttcagtt gacactaaat tttttccatt aagtcctgta gcaccatcta aaacccatgc
    33241 cccaaatgga tttataccga ctacatcttc attaaattta gctgttcctt gattacataa
    33301 taggctatag tttcttatat ttaaagccat atcaacctcc aaattgaata tctaaaaata
    33361 aagttctatt taaaattata cagcaaataa aaaaatctac aacaaaactt gttgtagatt
    33421 tttacttaat ctatattatc tttatacttt cagtatttta atgatggaat aatctattct
    33481 tagtaaatgc catgactatg ttatacttat tacaagttaa tataacatta tcatcacgaa
    33541 tagaaccacc tggttgtgct ataaaactta ccccacttct ttttgcacgt tctatattat
    33601 ctccaaatgg gaaaaatgca tctgaaccaa gtgctactcc tgttaaagtt ttaagccatg
    33661 ctctttttcc ttctccagtt agaggctctg gtttttcttc aaaaaagttt tgccatatgc
    33721 catcagcaag cacatccata taatcatcag aaagatatac atcaatagtg ttatctctat
    33781 ctggacgacc tatatccttt ttaaatttta agtttaatac tttaggatgt tgacgaagat
    33841 accaagtatc agctttgtta cctgcaagtc tagtacaatg aatacgtgat tgttgacctg
    33901 cacctacccc tattacttgt ccatctttag cataacaaac agaatttgat tgtgtatatt
    33961 ttaatgtaat aagagctata attaaatcac gttttgcact atctgtaaaa attttattat
    34021 cagttggaat atcttttaat aggtcttcat ttattaatat ttcatttcta ccttgttcaa
    34081 aagtaattcc ataaacatcc tttgtttcaa taggttcagg agtataattt tcatctattt
    34141 ttacaatatt atagttgcct ttacgctttc cttttaatat ttcaagggct tcatctgtat
    34201 atcctggagc tattatacca tcagaaacct ctcttgctat aatagttgca gtctccttat
    34261 cacatacatc tgaaagtgct ataaaatcgc cataagaaga cattctatct gctcctcttg
    34321 ccattgcata agcacaagct aatggagaaa gctctaaatc atcaacaaaa tatatttgct
    34381 ttaaagtatc tgataaaggt acccctactg ctgctcctgc tggacttaca tgtttaaatg
    34441 aagttgctgc tggaagacct gtagcttctt ttaattcctt cacaagttgc caactattaa
    34501 atgcatctaa aaaatttatg tatccaggtt ttccatttaa tacctctata ggaagttctc
    34561 cattcttcat ataaatcttt gatggttttt gatttggatt acagccatac tttaattcta
    34621 gttctcttgc catatactca tccctctcta taataaatta atcataagtt tgattaatcc
    34681 tatattaatt aaattattcg tactaataaa attatatggc atatttatcc attaatcaac
    34741 tatatttttt aaaactccta ttccttcgac tttacattca actatatcgc catgctttaa
    34801 atatacttga ggattcatcc ccatagcaac ccctgctgga gttccagttg aaattatatc
    34861 tcctggcttt agagtcattc ctcttgataa atcatttatt atagtaggta tatcaaatat
    34921 aaagtactct gtattagaat cttgtcttac ttctccattt actacactac ttatatctaa
    34981 ttttaaagga tgttcaaatt cttctttatg tactatacaa ggtcccatag atgtatgtgt
    35041 gtctaagctt tttcctctaa accattgaac atgtttatct tgaagtgctc tttcacttat
    35101 atcatttact attgtatatc caaatatata ttcataagca tcttcttttg atatatttat
    35161 accttcttta cctatgatta ctgcaagttc tgattcatag tccaacttct ctaccaaatc
    35221 tccatgtaag cttatcttat catccactcc aattattttg tctgctctct tagaaaaata
    35281 aattggataa tctggcataa ctacgttttt tataactcca tctgggattt cattgacatg
    35341 gtctttatag tttagaccta aacatataat atctttttta ggtcttacaa ttggggaaca
    35401 taatttaact tcttctaact tataacactt tgcgttttca aatgaattgt tttttatttt
    35461 ttctaaatct cctgtagata cgttttcaat aaaatcattc atatcagtat ataatttact
    35521 taatcctaaa ctattaattt catatatact atttgtttct gaattaaata cacctatttt
    35581 ttcttcattg ctactacaaa aagtcacaaa tttcattttt attcctccat tcaataagtt
    35641 gattaatatt atttaatttt tattagttta tataaattgt aattcataat atttatatgt
    35701 caatacatat ttatatattt taaaaatatt caaataattt ttattaaatt tattagttat
    35761 taatatataa gtgctttata aaacacaaat aaaataaggt tgatttaaaa ttaaatttaa
    35821 aacaacctta taaaccgatt atactatcta cctaataaat atagcaaata agctcctcaa
    35881 taatatacaa aaccctacat ctctaaacct atacttaact aatccaagta caataccaaa
    35941 aacagtacct atcataattt ttgaaataat aacttcaaag gtgtagcttg aattgttata
    36001 caatattaca taatttctta taacatctat gtatcctata ttataaattc cagatagtat
    36061 agatgttata cagataattt tactctttga ctttataaaa ttacttagat agttccatat
    36121 atattctcta aaaaataact cttcaaatat tgggactatc aatacagaca aagtcatcat
    36181 agttacatta aaaagtacat atcccttagt acaataagtt acaattattg ctaaagataa
    36241 aaccacaaca aaaagtatca gcttacactt ttcaataagt atattgtttt tattttcttc
    36301 aaaagcttct gtgatttttc tatttctaac cttgttatac aaagaattat cttttaaaaa
    36361 taaaattaac gataaaccaa ctaatgtaaa agaaattaag tttataatat taaaattttc
    36421 aagagtaaat tcaaattgac ttagaagtac ataattcaaa atcatcctta atatttgaac
    36481 tataaacatc aataaaatag aatctattat gcattttttg taactatact tcatcccaat
    36541 ttaaatcacc ccatgtatat gctaatcgta aagtccattt aataccctac ttacagttgc
    36601 tttgctccaa cttgtttgat ttgaaatttc tttatagctt aagccatctc ttcttaaact
    36661 ctttatatta tttatatcat catcagtaat ttgtttttct tttggcttaa ggctattttt
    36721 atagcttctt agtctatcca attctgattt caaactatct acctcaaact gttttttttg
    36781 aagtttactc tcataagaat ctctcatttc attaaactct ttaatgtagt ttactttcca
    36841 gtcctcatcc atttttgttt taaatatacc catactttaa cctccttaaa ttttgttact
    36901 caagttatgt atcgattata aacattaatt tctatattta tctttaaaat ccttgtacat
    36961 aacccctaga taagttagta aaattcagat atatttttac aattttaagt atatattaac
    37021 aatagaaaaa aatataatgt cttaatacaa catattatat actaaaagtt gttaaaatga
    37081 acctttttat agtctaaatc agagtcaccg tatgctttct tttcttcatc aggatatcct
    37141 atagaaatta tacattctac actgtacttg ctaggtactt ttaattcttc tctaatgtac
    37201 ttatcagctt ctatattatc atcatgcatt ctatttctta cttgtatcca acaagaacct
    37261 aaacctagtt catgagcttg aagttgtatg tatgaagcag ctatacttgc atcctctatc
    37321 caaacatcat ttttttcttt atctgcaata attacaatag ctaaaggtgc attttttaag
    37381 aaaaatccac cacctttagt tctacattga gataaattaa ttagcttttc tttatcatct
    37441 accactataa attcccatgt tctaagcccc tttgatgatg gagctaaaag agcagctttt
    37501 aatattttat ctaatttttc tttttctact tcaacatcct tgtattttct aatacttctt
    37561 cttttcttta gaaatccaat catataaatc atccctttca taatagcata cactaggtta
    37621 tattatgccc ttactaatgc tgaaaatcta ttatttatcc cttgtattat aaatgctctt
    37681 tataatattt tatcataaca gtagaaagat ttctcctttc ctaaaaaact catttctccg
    37741 atttttataa aaccacactt tgtaagtaat aaattcattt ttgagtttat tgaatatgta
    37801 tcgctcttta agtatgatac attgttttct acagctaatt tttcagcaaa atcaactaac
    37861 ttagatgcta ttcctgtctt tctaaagttt ggatttacag ccattctatg tattaccatt
    37921 gcttttttat tagatgacca atttaagcct aaatattctt ctggctcttc ataattaaga
    37981 catataaacc ctgctacttc tccatccact tcataaatat ataaatcttg tttcttaata
    38041 tctgaaacaa agtctttttc taatggatag ttttcatccc attgtgtatt attataagtc
    38101 ttcatttctt ccactgtaga ttttataatt tgcataatgc tttctaaatc attcatattc
    38161 gcttttctaa tcataaaaat atctccttaa gttaatgtat ttatttacta tctctttact
    38221 ttaaaccatt cattttctac gtttttgctc attttttcta ataacttagt taccacttgt
    38281 ttttcttcat ctgtaaaatt atcaaaacat atctctatac ttctattctc ttcttcaatt
    38341 atcaactcat aaacttccaa agatttatca gttggagtta aattatatcc tcttttatca
    38401 aacttatcct gctttttatc tacatatcct gcctttatta atttttgtat agccttggtt
    38461 gttgtagcct tgtctacctt taacatattt gataattcca caagatttat accaggattt
    38521 tcacagattc ttgtcaaaaa tgtaaattgt cctttttgta actttaattc cttgtactta
    38581 atatcattta ttgaatttac tgctctagat aaagtaccta cttctcttaa tatattacta
    38641 tctaaagtct taatcaaaca cacctccact gaaactacta tggctaaagt caaatagttt
    38701 ctgttttata aaatttcaca taatcaatat attcattttt agttgaaagt tcaacttttt
    38761 taaattttac catattttgg ttgaaagttc aattaaaaat aataaattta tttaatcttt
    38821 ctttgcatat taatcaatta attttaaaat taaaatggat atcccaaaat taactttttc
    38881 gtttatgagg aactctcttt tgtataaaat caaatatatt ttatcattcc aataaaaaag
    38941 gcttatctct attttgagaa agcctctttc cttcaatcta tattttactt tttccctaaa
    39001 taaaatctat acaatatact tacacacctc atccgcaaac tctgtagtag aagctgagcc
    39061 acccaaatct acagtcaatt tacttccttc aagaaatact ttttctaaag ctgtttctat
    39121 tttttgtgca ttttcatatt cacctaagta tctaagcatc attactgatg attgaataag
    39181 tgctgttgga ttagccttat tttgacctgc tatttgtgga gcacttccat gaacagcttc
    39241 aaatatcgca caatctttac caatatttgc acttggaatt atacctaaac ctccaacaag
    39301 cccagcacct aaatctgata gtatatctcc ataaagattt ggcattacca ttacatcata
    39361 attttctgga tttaaaacta agttcatagc tgctgcatct acaatcaaat catcatattc
    39421 tacaccatgt tttgatgcaa cttctctaaa cacatctaaa aacaatccat cagacatttt
    39481 cattatattt gccttatgta tagctgtaac tttttttctc ttattatctt taacatagtt
    39541 acatgcaaaa tcaacgattc tttcacttgc acttcttgtt attatcttta tactttcagc
    39601 tgcataatca cctattttat gttctattcc agcatataaa tcttctgtat tttctcttac
    39661 aaccactaaa tcaacatctt catatcttga ttttatcccc ttaaaacttt taataggtct
    39721 taaattgaca tacaaatcta atgcctgtct taaagttaca tttacactcc taaatccttt
    39781 acctacagga gtagtaattg ggccttttat agctattttg ttctttttta tactatctat
    39841 aatatattca ggaagtggag taccatactc ttcaataact gcctctcctg cattaacttc
    39901 ttcccattct atttctactc cagtagcttc tactaccttt ttcatagctt tcgcaacttc
    39961 tggtcctatt ccatctcctg gtataagtgt cactttatac attcaatatt ccctcttttc
    40021 atattaatta gcttgatttt tagtgtagtt taattttcca ccagctttta atacatcaac
    40081 ttcttttgct gacaattcta ctttgacctt aaaagaagtt cctttagttg tattcttaac
    40141 ttctaaaatt ccactaccta gattgtctaa aatatttggt atctccagat tatctaaaag
    40201 agatatattt tcataatctt tctcatcaca aaactccatt ggaattatac cactatttat
    40261 caagttagcc ttgtggattc ttgcaaagct cttaactatt acccctttaa cacctagata
    40321 taatggtgct aaagctgcat gttctctact tgaaccttga ccataattat ctccaccaac
    40381 tataaatcca ccattattgt cttttgctct ctgaggaaat tctgtatcta cagtattgaa
    40441 acaatagttt gcaagatacg gtatattact tctaaaagga agtaacttag catttgatgg
    40501 cattatatgg tcagtagtta tattatcttc tgtctttaat ataacttttc cacctataga
    40561 ctgacttagc tctgtattaa gagggaaagg ttttatatta ggtcctctaa ctacttctac
    40621 atcacttcct acatcagcag gtttaattat catgctatca tctatcaaaa attcatttac
    40681 atctaaattt gtaaatttaa tatcaaattc tcttgggtct gtaagcactc ccttgattgc
    40741 acttactgct gccacttctg gacttactaa atatacttgt gctgacaaag tcccacttct
    40801 accatagaaa tttctattga aagttcttaa agacacagag tcagtacctg gagattgacc
    40861 cataccaata catggaccac aagaattttc tagtattcta gctccagcac ttattatatc
    40921 tgccaaagcc ccatttctag caatcatctc cataacttgt cttgaacctg gtgctattac
    40981 caaactcaca tccttatgta ctttatttcc tttcaggatt tgtgctactt tcattaagtc
    41041 ttcataagaa gaatttgtac aacttcctat tgccacttgg tctattttaa tcttgcctat
    41101 attttcaact tcatctacat tgtctgggct atgaggtttt gcagctaatg gttttaattc
    41161 atccaagttt atagtaatct cttcatcata aactgcatct gcatctggtt ttagctctat
    41221 ccatgcctct tctcttcctt gactcttaaa aaattctagt gttttttcat cacttgggaa
    41281 gattgaagta gttgctccaa gttcagcccc catatttgtt attgtagctc tttgtggaac
    41341 agataggctt ttgacacctt ctccaccata ttcataaact ttacctactc caccttttac
    41401 agtttgtttt cttaacactt ctaatattat atcttttgat gataccatat tatttaattt
    41461 tccaactaga ttcaccttac agacttttgg tgcttttata tagtatgctc ctcctgccat
    41521 ggctaaagca acatctaatc caccagctcc aatagccatc atgcccacac ctccagcagt
    41581 aggtgtgtgg ctatcagaac ctaaaagtgt atctcctggt gttgaaaatc tttctaaaaa
    41641 tacttgatga caaataccat ttcctggctt agagaaataa actccatatt tatctgccac
    41701 tgtttgtata tacttatggt cgtctgcatt ttcaaatcct tgttgtaaca tattatggtc
    41761 tacaaatgcc acacttcttt tagtctttac tttatctatt cccatagctt ctagttgaag
    41821 atatgtcatt gttccagtag aatcttgtgt taaagtttgg tctattttta taccaattga
    41881 acttccagca acagcttctc catctactat atgtttttta attattttat aaactatatt
    41941 atctcccata ttttcctcct aaacaattat atcttaaata tatgaacctc taactgcttc
    42001 ttcttctgtt gtgcctttct catgtgctag tatatcacaa tataattgaa gaatttcttt
    42061 atccattaaa cttcttttta atcttataga tgtagctctt atagcattta atagcttggt
    42121 agcgttttct ggagaaatat acatttcata ttcactcaac ttatttacaa ctgcagctct
    42181 tcctgaatgt ttacctatta ctatttttct ttcaagattt acttcacttg ggtcaaatgg
    42241 ttcatagtta tgtgggtctt ttaaagctcc atctgcatgt ataccagatt catgtataaa
    42301 catattatcc ccaactactg gtttccatgt aggaagtatt cttcctgatg cctgtgctac
    42361 atactcacat attcctctaa attttctagt atctatatta tttaaatcac atttataaac
    42421 acacttaagt gccattaaaa cttcttctaa tgctgcattt cctgctcttt cacctaatcc
    42481 atttactgta acaccaacat aatttgctcc tgctgctatt cctgcaagtg catttgcagt
    42541 tgccatccca aagtcattat gtgtatgcat ttctatatca aaattagttc tttcataaag
    42601 agtttctatt gcattcttta tagaaaaagg attcataact cctactgtat cacagtatct
    42661 aaatctatct gcccctgctt gttttccaac atttataaat tcagttaaga agtctatatc
    42721 tgctcttgaa gcatcttctc catttactga aacatataat ccatttttct ttgcaaattc
    42781 aactgtttta gccatatttt ctaaaaccca gcctctagaa gttctcaatt tattttctat
    42841 atgaatgtca gatactgata ttgatatagc tactgcatca acaccacaag aaatagattc
    42901 ttcaacatct tttattactg ctctattcca agccattata tctgatttta aatttctact
    42961 acatatgtgc tttattacat ttttttcatc tccacccata gtaggtatac caacttcaat
    43021 ttggtcaaca cctaggtcac ttaacatttc agcaatcatt attttttctc tatttgcaaa
    43081 tacaacccca gctgtttgct ctccatctct taaagtagta tctactattt ttatttcctt
    43141 tgctctatct tttgatataa cgcacatatt aatgcccccc ttagtatact tttaatttta
    43201 aaacattaag tcttattttt cattgatacc tctaacttat gattattttt ttatttttta
    43261 ttcattttaa tttcatttgc aatttatttt tttagctttt catatcttgt aatctattat
    43321 attactttgt atcatttatt gcactatatt ttttcttttt tgtgaaaact tcttaaaaat
    43381 gaaagttatt atgtttttct tgcattttaa tttaattttt aaatagattt attttcaaaa
    43441 aacctttaaa aataaaccta taaaattcct actaaaattt aatttaagtt acattccatt
    43501 ttaaagtatc ttaatatatt tttaagctat ataaataata attaaaaaat ttactaaaaa
    43561 tacttataaa tatgatatta ttaattggtt aataaaaaag aaaatttttc atgaaaggta
    43621 agataacaat ggatttcaaa agcttaggga ttagtgaaaa tacaataaat attctaaaaa
    43681 aatcaggaat aacaacacct acacctatac aaaaagaaag tattaaacta ataaaagaag
    43741 gaaaagatgt aatagcagaa gctcaaactg gaacaggaaa aacactagct tttttacttc
    43801 cgatatttga aaatatatca ttagatataa atgatataca agtacttata ctttctccta
    43861 caagagaact agctattcaa ataacagaag aagctatgaa acttaaagaa tctaaagatg
    43921 taagtattct tgcagcatat ggaggaaaag atattggttc tcaaataaag aaactaaaag
    43981 gaaatattca tatgattata gctacaccag gtaggctttt agaccatcta aatagaaaaa
    44041 caatagacct tagtaagtta aagacttttg ttttagatga agcagaccaa atgttgctta
    44101 tgggatttaa aaatgaagtg gaagctattt taaaagagac ttcaaacaaa aagcaaacac
    44161 tatgcttttc tgcaactata aattctcaag taaaaaaatt agcttataga tacacaaaaa
    44221 atcctgttgt agtttctatt caaaaagaag aaatcactct taataatata aaacaagaag
    44281 tagtagaaac tacagataga aaaaaattag atgccttatg taaggtacta gatgaagata
    44341 atccttttat ggcaataata ttttgtagaa ctaagagaag ggttgacaat cttgaagaag
    44401 ctcttgctat aagaggatat aattgtcaaa aacttcatag tgatattgct caatcaaaac
    44461 gagaaagaat aatgaaatca tttagaaatt tagatataca atacttaata gctacagatg
    44521 tagcttctcg tggtcttgat atatctggag taagtcatat atataattac gatttaccag
    44581 aaacaccaga agactacatc caccgtattg gtagaactgg tagagctggt gaagaaggct
    44641 atacatgtgc ttttattgac cctaaaaatg aacgaatgtt aagtgaaata gagacggcta
    44701 tagaatctaa aattagtaga agaattatag aattataatt tatagattgc ttaaaatacc
    44761 aatgccacac aaacactgta atatatagaa agctacagaa aacatgtatc aatctatact
    44821 ttgctgttat actattttat attaaagaaa aaagaggcta tccaaagtag gattagtctc
    44881 ttttattgtc aaaataatga aaatatattt gacttcatat aaaaaagagt gccttataaa
    44941 ctaaaagtcc attttgagac accctctttt tatcttttat tcttagtttt atattgcttg
    45001 tttatagttg tttaatatta gattttgcaa ataatcttaa taaacatgca gatatattat
    45061 ctatagtatt cttatctata gtattctctt tagcaatatt ttttgctact acatctaatt
    45121 tacattttca tttaattccg ctggccattt agcttctgga ttttcctttg caaacttctt
    45181 attcttataa tgtcctagtg taaggaatgg aattactata gtgaatagac caagataacc
    45241 acaatatcca tatccatatt taacaatatt acttaatcct gtaagtgata taagcattga
    45301 cactaccatt attgcacaag ctgtaaatac ccttcttttc attcttgaag aaaggaaaga
    45361 aagtgcttta tggtcttcaa atctatttac aagaccaaat atagtagtta ctcctgtaga
    45421 cattaaacaa aggaataatg caacattata aattatgtat atagtatgat ttccagattg
    45481 acctgcaaca taaagagtag ggagtgttgt ttgcccagca gcaataattt ctctatacca
    45541 tcccattagc atagttacag caagtcctaa tgcaattgca ttcataacaa atgaaattat
    45601 cattgcctta gtagcttctt ttgagttctt aagaagtggt cttgaacatc ctgcaagtgt
    45661 aggtataaca accgattgaa aacctgcata aataaaagta ttaagtattg gcttgatata
    45721 gccaggtgtg aaactcgttc tatttgataa tatttcagtt atttctggta ctttagcttt
    45781 tataccaaca ataaaaatag taagagtaca tactagaata gctattgtca ttgctgtagc
    45841 tgctttagat acaagcatag caccaaatat agtaaagaca agaagtacaa ctccaataat
    45901 aaatacagct acaaaataat ttactcctat actttgaaat actgctgctg cacctgctat
    45961 aacagcactt acagctatta aaaccattag atagtaatat atctcccata ttatttcaag
    46021 ttttggatat ggtctccaaa gctcacaaaa taaatcctta taattcctac aattgttatt
    46081 attatacatt atgataactt ctctcatggt agcagtaagt ataatcattg ctaaaattgc
    46141 cataagtggt gctgtccatc catactgcac atagtactga gtttcttgat tccctgtagc
    46201 aaatcctcct cctgcatgtg aactaaaaag aacgcttgca acaccaaaaa tggctaacat
    46261 agagcccgtt acttttgctt tctttacact tttttgcatg aaatcatcct ccctaaaatg
    46321 atattaaaag tttttatttt aacactttat tgataaaaca ataacatttt tgctcattaa
    46381 attcaactat atttttccaa tgactttaca ttttcaactt atttctacct ttatattaaa
    46441 t
//
LOCUS       DAMJWS010000022        45613 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMJWS010000022 DAMJWS010000000
VERSION     DAMJWS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.022"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..609
                     /locus_tag="PC399_001308"
     CDS             <1..609
                     /locus_tag="PC399_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HDF2592181.1"
                     /translation="PTGATGATGVTGATGPTGPTGATGATGATGASAIIPFASGIPLS
                     LTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITSISA
                     YFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGI
                     VTGLNIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            778..1539
                     /locus_tag="PC399_001309"
     CDS             778..1539
                     /locus_tag="PC399_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2592182.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1848..2126
                     /locus_tag="PC399_001310"
     CDS             1848..2126
                     /locus_tag="PC399_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592183.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2419..2994
                     /locus_tag="PC399_001311"
     CDS             2419..2994
                     /locus_tag="PC399_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF2592184.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(3100..3933)
                     /locus_tag="PC399_001312"
     CDS             complement(3100..3933)
                     /locus_tag="PC399_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF2592185.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(4001..4630)
                     /locus_tag="PC399_001313"
     CDS             complement(4001..4630)
                     /locus_tag="PC399_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF2592186.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            4941..5600
                     /gene="cprR"
                     /locus_tag="PC399_001314"
     CDS             4941..5600
                     /gene="cprR"
                     /locus_tag="PC399_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF2592187.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            5811..6869
                     /locus_tag="PC399_001315"
     CDS             5811..6869
                     /locus_tag="PC399_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2592188.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            7120..9264
                     /locus_tag="PC399_001316"
     CDS             7120..9264
                     /locus_tag="PC399_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7534..7536,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HDF2592189.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            9313..10146
                     /gene="fdhD"
                     /locus_tag="PC399_001317"
     CDS             9313..10146
                     /gene="fdhD"
                     /locus_tag="PC399_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF2592190.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            10115..10330
                     /locus_tag="PC399_001318"
     CDS             10115..10330
                     /locus_tag="PC399_001318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592191.1"
                     /translation="MQIHRELSKSENEYMCTKKEEQRYSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            10388..10939
                     /locus_tag="PC399_001319"
     CDS             10388..10939
                     /locus_tag="PC399_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2592192.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRQCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            10964..12340
                     /locus_tag="PC399_001320"
     CDS             10964..12340
                     /locus_tag="PC399_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2592193.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            12340..12855
                     /locus_tag="PC399_001321"
     CDS             12340..12855
                     /locus_tag="PC399_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2592194.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNLDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(12933..14216)
                     /locus_tag="PC399_001322"
     CDS             complement(12933..14216)
                     /locus_tag="PC399_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF2592195.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            14404..14826
                     /locus_tag="PC399_001323"
     CDS             14404..14826
                     /locus_tag="PC399_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF2592196.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            14928..15866
                     /locus_tag="PC399_001324"
     CDS             14928..15866
                     /locus_tag="PC399_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF2592197.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            16052..18076
                     /gene="ligA"
                     /locus_tag="PC399_001325"
     CDS             16052..18076
                     /gene="ligA"
                     /locus_tag="PC399_001325"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF2592198.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            18236..19585
                     /gene="rph"
                     /locus_tag="PC399_001326"
     CDS             18236..19585
                     /gene="rph"
                     /locus_tag="PC399_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF2592199.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKIIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            19585..20055
                     /locus_tag="PC399_001327"
     CDS             19585..20055
                     /locus_tag="PC399_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2592200.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            20249..21535
                     /locus_tag="PC399_001328"
     CDS             20249..21535
                     /locus_tag="PC399_001328"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF2592201.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            21685..22269
                     /gene="clpP"
                     /locus_tag="PC399_001329"
     CDS             21685..22269
                     /gene="clpP"
                     /locus_tag="PC399_001329"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2592202.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            22288..23538
                     /gene="clpX"
                     /locus_tag="PC399_001330"
     CDS             22288..23538
                     /gene="clpX"
                     /locus_tag="PC399_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF2592203.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(23609..24136)
                     /locus_tag="PC399_001331"
     CDS             complement(23609..24136)
                     /locus_tag="PC399_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2592204.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(24124..24636)
                     /locus_tag="PC399_001332"
     CDS             complement(24124..24636)
                     /locus_tag="PC399_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2592205.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            24816..27179
                     /gene="lon"
                     /locus_tag="PC399_001333"
     CDS             24816..27179
                     /gene="lon"
                     /locus_tag="PC399_001333"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF2592206.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            27169..27780
                     /locus_tag="PC399_001334"
     CDS             27169..27780
                     /locus_tag="PC399_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF2592207.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(28046..29416)
                     /locus_tag="PC399_001335"
     CDS             complement(28046..29416)
                     /locus_tag="PC399_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2592208.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFVGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(29591..29706)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            29994..31073
                     /locus_tag="PC399_001336"
     CDS             29994..31073
                     /locus_tag="PC399_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2592209.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            31465..32145
                     /locus_tag="PC399_001337"
     CDS             31465..32145
                     /locus_tag="PC399_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2592210.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            32138..33517
                     /locus_tag="PC399_001338"
     CDS             32138..33517
                     /locus_tag="PC399_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2592211.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            33688..34773
                     /locus_tag="PC399_001339"
     CDS             33688..34773
                     /locus_tag="PC399_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2592212.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            34827..35186
                     /locus_tag="PC399_001340"
     CDS             34827..35186
                     /locus_tag="PC399_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2592213.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            35239..36111
                     /locus_tag="PC399_001341"
     CDS             35239..36111
                     /locus_tag="PC399_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF2592214.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYISNIGMII"
     gene            36112..36555
                     /locus_tag="PC399_001342"
     CDS             36112..36555
                     /locus_tag="PC399_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592215.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSRHTLPTTDKTREKFSKIKLNEIKKIYETIGINIITKISIDK
                     NKLEIEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            36570..37154
                     /locus_tag="PC399_001343"
     CDS             36570..37154
                     /locus_tag="PC399_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592216.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANLLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            37407..38444
                     /locus_tag="PC399_001344"
     CDS             37407..38444
                     /locus_tag="PC399_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2592217.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            38525..38890
                     /locus_tag="PC399_001345"
     CDS             38525..38890
                     /locus_tag="PC399_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592218.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            38895..39212
                     /locus_tag="PC399_001346"
     CDS             38895..39212
                     /locus_tag="PC399_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592219.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            39260..40864
                     /locus_tag="PC399_001347"
     CDS             39260..40864
                     /locus_tag="PC399_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592220.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            40988..42616
                     /locus_tag="PC399_001348"
     CDS             40988..42616
                     /locus_tag="PC399_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2592221.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTSLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAALDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNIYFSEEGQSELD
                     VMYNRVVSAYTYALESMRSDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            42881..44230
                     /locus_tag="PC399_001349"
     CDS             42881..44230
                     /locus_tag="PC399_001349"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF2592222.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            44386..45465
                     /locus_tag="PC399_001350"
     CDS             44386..45465
                     /locus_tag="PC399_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2592223.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT        18551 a         4916 c         8113 g        14033 t
ORIGIN      
        1 ccaacaggag caacaggggc aacaggagtt acaggagcaa caggcccaac aggcccaaca
       61 ggagcaacag gagcaacagg agcaacaggt gctagtgcaa taataccttt tgcatcaggt
      121 ataccactat cacttacaac tatagctgga ggattagtag gtacacctgg atttgttgga
      181 tttggtagtt cggctccagg attaagtata gttggtggag taatagacct tacaaacgca
      241 gcaggaacat tgactaactt tgcattttca atgccaagag atggaacaat aacatctatt
      301 tcagcatact tcagtacaac agcagcactt tcacttgttg gttcaacaat tacaattaca
      361 gcaacacttt accaatctac tgcaccaaat aactcattta cagctgtacc aggagcgaca
      421 gttacactag ctccaccact tacaggtata ttatcagttg gttcaatttc tagtggaatt
      481 gtaacaggat taaatatagc agcaacagca caaactcgat tcttactagt atttactgca
      541 acagcttcag gtctttcatt agttaatact gtagcaggat atgcaagtgc aggaattgca
      601 ataaattaga ttagataaga aaaggcttct aatatcgtat tgatattagg agctttttta
      661 agaatgattg atgaacatag ttttaatatt aattagtgcg catattataa aatactatta
      721 tataatataa aataataaca ttaaattgga aatatagaat tatctagggg tgatactatg
      781 aaaaataaga ttgaaatatc aatgaacaac tttgtaaaaa attatcataa atcaaataat
      841 ataactacaa agtggcaaga acttttgttt gcttatgcat ctgctgaaga tgatatgttt
      901 cagaagctaa aaagtgttgt aggaacatct catgctatgc caaaagattt tctggaaaat
      961 ggaaaaacta taattacata ttttatacct tttgatgaaa gtgtaaataa tagtaatatt
     1021 ggagggtttg aatgttctga aatatgggca agagcgtaca tagaaactaa tatgttgatt
     1081 tcaaatttaa atgatttctt atgtgaagaa ctagagaaga taggggctaa ttcatatgct
     1141 attcctgggt cgtataattt tgacagtgaa aaattggtaa gtgactggtc acaaagacat
     1201 gtagcattca tagcaggtct tggaactttt ggtttaaata atatgcttat tacagaaaaa
     1261 gggtgttgtg gaagaatagg aagcataatc acaagtttag aattagagcc aactaaaaga
     1321 ccagaaaatg aatactgctt gtataaacat aataatagtt gcaaaaaatg tgtcaataaa
     1381 tgcgtgaaag aagctttaaa agaagatgga tttaatagga ataagtgtta tgaaatgtgt
     1441 atgataaatg aaaaaaaata cacttatata ggtggatgtg atatttgtgg aaagtgtata
     1501 gttggagttc cttgttcagt aataaaccca tcaagataat ggatttaagt tgactaataa
     1561 ttttaaagat agtttataat gaaaaaattt tagttatcaa aataaatata atataaaaac
     1621 taaataaatg tgtttaaaaa atggataaat aatttgtgtt gattgacaac cagttatact
     1681 gttgatagac ttatatgtat cgctgtgata tataaggagg gttgagattt tatgtttgat
     1741 ttaatcttta tttttgcata gcaagggcta ttgcaactat gaaatttaat atataaatag
     1801 ccctttgcta ttcctaatga aaaattacta tttaatagga gagcaaaatg agctataaaa
     1861 aagcaataca tatattacca gaagaattac ttgaattaat acaggaatat gtagatggtg
     1921 aatgtatcta tattcctaga aaatctaata ataaaaaaga atggggaagt aatacaccta
     1981 ctcgtgagga gttgtcaatt agaaatatgc agatttacga agactatcaa gttggatatg
     2041 atttgcaata tttatcggaa aagtattatt tatctttgaa aagtatccaa agaattgtat
     2101 tacaagaaaa aagaaaagat atctaaggat gagccagtat agttttataa ttatactggc
     2161 tcattttctt ttgataaaag tgtttgaggt atagtttctt ttaagtggat gatataattt
     2221 aagtaatttc aataacttat acagtagcta acaaagctgg ttgtaaagta tctaataaga
     2281 agtagtagct ttttatatgt ttgaaattaa aatttattaa aattaggagg tcttgttttg
     2341 aaaaaaatac ttgattatat tccacctttt cataactttc tattaattta gtaatcttat
     2401 agaaaggaaa ttgcatttat gaaaaaaatt acttgggagg tggaatatat ctcccagtta
     2461 cctgtaatta ggtattgtaa aaaatgtgga aaaaagactg aatatatttg ttcaggttta
     2521 tttagggtta atgctcaaca taaatatttg gatatatggt taatatacaa gtgttcaaat
     2581 tgtgattcaa catggaactc aactatatat tctcgtgtta atccaaaaag cttaaatcca
     2641 gaaatactag aacaattcca tacaaatgat tataatcttg ttaagcaata tgctatgaat
     2701 atggaacttt tacatagaaa tggagcagaa gttggtattc cagagtataa aatagtagga
     2761 caagaaatta atattaatga ccttacacaa gttcatatta ttagtaaata tccatgtcag
     2821 ttaaaggtat caacattatt acgtgagaaa ctaggtttat ctaggaaggt atttgacgaa
     2881 atgttggatt gtgggattat tagaagtaac tcaggagttg atttaaagaa aggtaaatta
     2941 aattatgaaa ttatgttaga tataggcaag gagaagcaaa agtcttcaaa ttgattagaa
     3001 taactaactt aatatatttt caggaacatc atttaaaaaa atagtgactt taaaaaattt
     3061 aaagccacta ttttttaata tgttttatct atataactat tatttttcta ggttttttaa
     3121 tatttcgtaa tagtgtgttg tggctatttc acttgactcg ccttttttac aaaaagataa
     3181 tttagatagt tttggaacta cacgttcaac gacaatttta ttacctatca ttttagattc
     3241 aagtatcttc aatgcttcta aaaatctctt atcctttttt gcacgattat aaaatgataa
     3301 aacgtagaca taaaggaata ggttataatt acggaaagga tattcaactt gcataaataa
     3361 tgtgccaatt ccatagtgac aagggccgat tggtttttta atagtccaat ggtcaagtag
     3421 aaaatctaca gctttgtcaa gttttgattc tttatttaga taattactaa aacggaatgc
     3481 atttaatata ttaagagttg gtagtggatt tgagtattca gtttctggac ctcgaccaaa
     3541 actaaattta ttgcaacgcc acccaccatc agtatactgt gtatctaaaa aatgttgaaa
     3601 cgttttttgc attctaatgt cagatgcata tcccatatga cagagtacgt tagcagcatt
     3661 tgcagtatga caagggtaaa tacctccatt tggatatagt ttgaaacgtc catcttctcg
     3721 ccatgtgcta aatatcaggt cagcacattc tttaagtaaa gtatcatcag ggttcacacc
     3781 taattctaat agataaagta cactatcaag agtagagaaa ggagaacctt ttattaaacg
     3841 cttatcaggt gtagtccaat agtctgcacc attatcatac ctgtgtgata atattatttc
     3901 aatatctgat aaagattgat tttctacatt cattttttac tccatttcat attatattct
     3961 attaaatcta tcaaaaactt tattataata ttatttatat ttataattga tttattttat
     4021 ttaaaattgc atttatagct gtatcaggaa caactgctaa atcttctgca ttgaagtaat
     4081 aaactatatt agaatcaaat aatatatttg aatttggagg aaattcatca tctccaaacc
     4141 aaagtataaa taccatagat gtattaccca tatatgtaaa cctataagct aaatctccca
     4201 tgtttacttt ttcagctcca attttctcca taactaactt atatttgtct atttgagatg
     4261 taaaaacttc tgaaagccta tatagagtcc tgtttaaaaa atttggataa taaacattgc
     4321 ctcctggtat ttctttaaaa gttacatatt tattagttct aggaatacct ttagcattga
     4381 ttaaatatct aactatcaag attttaagct caaagttatt gcaaaggcta ccattactat
     4441 tccaaacttc tccacttgga tagtctataa tatattgtct atcaaaaaaa ttgagtgtga
     4501 atctatttaa atcattattg aaaggtatat tacttaaatt actaattgta tgagggtcta
     4561 attctttgag tttttttcga acatagtcaa atggaacatt ataattattt tttgttatct
     4621 tttcatccat aattttttcc tccaatttaa acaattaaaa gtatatttat aaaaagtttt
     4681 cttattatta attttattat atattcaaaa tattacaata ataaattgtt aaataaattt
     4741 ttatatttaa gtaaatacgt gtaaaatgaa tataaagttt ttgaaatata aagtttttgt
     4801 atctaaaata ttttatttgt aaaatagtaa ttaattattg taagctataa tatgagagta
     4861 ttatggttaa tgtgaatttt aaagatatta ttaaatatat aaattagaat actattaaac
     4921 ggataaatgg aggagttatt atgtctaaga tattagtagt agatgatgag ttagatatgt
     4981 taaagttgat tgaaaatgtg ttgaaaagag atggacatga agtaaaggta attagtgatg
     5041 tagaaaaagc tatgaacatg gattttaatt attttaatct aataatatta gatgttatga
     5101 tgcctaagat ggatggtttt gaagtatgta aaactataag aaataaggtt gattgtccaa
     5161 tactgttttt gacagcaaaa aatatggaaa gtgatattat gtacggtctt ggtataggtg
     5221 cagatgacta tataaccaaa ccttttggtg taggagaatt aagggctagg ataactgctc
     5281 atttaaggcg agaaagtaga gaaaaaaaga atttattaac tatctcaaat gttcaattta
     5341 actttttagg caaagaagtt tcaattgatg gtaaaaaaat taattttact aaaagtgaat
     5401 atctaatatg tgaatttcta gcaaaaaata agggacaagt attttcaaaa gaaagaatat
     5461 atgagtcggt atatggtttt gatggagaaa gtgatataag tactatcaca gagcatataa
     5521 aaaatatacg aagcaaatta aaacaatatg atatttatac aatagaaact gtatggggga
     5581 tagggtataa atgggtgtaa taagtataag tgttgtttag tgaaaatatt atatatatct
     5641 acaaaatgtt tgtaaaatat taatctatat attaaaattt aatttataga ttaatatttt
     5701 ttgtaaatga caaaattgta aggtattttg ttataaaata ttaagattca tagtgtatac
     5761 taataatata gtatttagta taaatcttga gcaaaggatg gttatttcta atggatagac
     5821 taaagaatat tagaaagagt ttactacagt atacattttt atttttgtta ataggtataa
     5881 caatttacct agtgtttaaa acattggata ttaaaatgct atcaaatgtt atggttatgg
     5941 tagataagaa gtttttattc ataggtgctt tggcaataat gtgtcacata atgcttgaag
     6001 gagtagtaat gaagataatt atagaaagca ctcacaaagt taatattaga tttattggtt
     6061 ttaagctagc cattatggga ttttattata atttaataac tccttttgct tcaggaagcc
     6121 aaccagtaca gatatatgta cttaaaaaat gtaaaatgcc acttagtaag gcaagtgcag
     6181 ttgttaccaa taagtctatt atcttccaaa tagtagttac tttttattgt acaatacttg
     6241 ttttaatgaa ttttgctatg ttagaaaaac aaatgaaaat gattatgcct ctagtgtttc
     6301 tagggatagc cattaattca tttgtcttaa ttatgataat attagttatt ttaaatccta
     6361 aaaaaataaa atattttatg agaattacta taaagcattt atcaaaaatt aagtttttaa
     6421 aatttttaga taataagatt gaatccatag aaagttttat agatgattat agtaaagcaa
     6481 taaatttttt tgcaaagaat aaaaaggtat taatttcaac tataatagta acttttattc
     6541 aattaagtgc ttactttagt gtttcatttt ggatatataa agcatttaat ttacaaggtt
     6601 atagctatat ttatatactg acattacaag catttttata tatggcaata tctccaatac
     6661 caacacctgg gaatgtagga gctaatgaat tagccttttt cactatattc aaatcagtat
     6721 tccctcaacc tttgatggga tatgcagtat ttttatatgg tggatttatg tactatttga
     6781 ttctgattgg aagtggaata tttactgtaa ttactcacta tagaatgaaa aatagaattg
     6841 gcagaaatat tgttttaagt gaaaactaaa taagtttaaa gaatatatat tgagtatatt
     6901 aaaaaatatg tcataagaac tagaatattt taattaaatg gttgaaatat aaagattatt
     6961 atgacatatt ttttttgaaa tttaagagga attgtgagaa aattgttgaa tttaatagat
     7021 agtattgcaa aatatttatg attttgcata ttataaaaac actcattatc tttaagataa
     7081 attttactta tcaggggata aatctaaggg gggaattgta tggagaaaaa aattttaaca
     7141 gtttgtccat actgtggagc tggatgccaa ttatatctag tagtaaaaga taataagata
     7201 ttaagagctg aaccggctaa tggtagaaca aatcaagaaa gtttgtgttt aaaaggtcgt
     7261 tatggatggg attttctaaa tgaccctaaa attctaacac ctaggctaaa aaagccaatg
     7321 ataagaaaac aaggtgtact agaagaggtt gaatgggatg aggctataaa ctatacagct
     7381 tcaagactaa atgaaattaa agaaaaatat ggtcctgatt ctattatggg aacaggttct
     7441 gcaagaggac ctggtaatga agcaaactat ataatgcaaa aatttatgag agctacaata
     7501 ggaactaata atgttgacca ttgtgctcgt gtctgacatg caccatctgt agccggtctg
     7561 gcttactcat taggtagtgg tgcaatgtct aattctatac cagagataga aaatgcagat
     7621 gttttattta tatttggata taatggagca gattcacatc caatagtggc aaatagaata
     7681 gtaaaagcta aaaaaaatgg tgctaaatta attgttactg accctagagt aactgaatct
     7741 gcaagaattg ctgatataca tctacctata aaaggtggaa caaacatggt tttagtaaat
     7801 gcttttggaa atgtattaat agaagaaggt ctttacaata aagaatttgt tcaaaatcat
     7861 acacaaggat ttgatgagta taaagaaatt gttaaaccat atacagctaa atatgctgaa
     7921 aagattacag gaatcccaga agagcttata agaaaagcta tgagagaata tgcaaaaggt
     7981 aaaaaagcta tgatacttta tggaatgggt gtctgtcaat ttggtcaagc tgttgatgta
     8041 gttaagggac ttgcatcaat tgctctactt acaggtaatt ttggtagaga aagcgttgga
     8101 ataggacctg tacgtggaca aaataatgtt caaggagctt gtgatatggg agctcttcct
     8161 aatgtatatc ctggctatca aaatgtaact gatgataaga taagagaaaa gtttgaaaaa
     8221 gcttgggggg taaaattatc tcctaataat ggttatagtt taactcaagt tccaaattta
     8281 gtactaaaag agaaaaagct taaagcttat tatatatttg gagaagaccc agtgcaaagt
     8341 gacccagatg catcagaagt tagagaagca ttagatgaat tggaatttgt cattgttcaa
     8401 gatatattta tgaataagac agcacttcac gctgatgtta tacttccagc tacttcttgg
     8461 ggagaacatg agggagtata tacatgtgca gatagaggat tccaattgat gagaaaagct
     8521 atagagccac aaggagatgt aaaaccagac tggcaaatta taagcgaaat ttctacagca
     8581 atgggatatc ctatgaatta taaaaatact aaggaaatat gggacgaact tagacaatta
     8641 tgtccaagtt tcttaggtgc tacttatgaa aagatagaaa ctcaaggatg cgttcaatgg
     8701 ccttgtaaga gtgaaagtat ggaagataaa ggaactatgt atctgtatga aggtcaaaag
     8761 ttttctactc ctaatggtaa aggtaatttg ttcgctgctg aatggagacc ccctatggaa
     8821 gttgaagatg atgaataccc atttagttta tgtactgtaa gagaagttgg acactattca
     8881 gtaagaacaa tgactggtaa ttgtagaaca cttagttcat tagaagatga accaggacgt
     8941 gttcaaataa attctaatga tgctgaaaaa ttaggaattg aagatgatga attagttaga
     9001 attagttcaa gaagaggaag tgtaataaca agagcaacag ttactgatag agttaaggaa
     9061 ggtgcaacat atatgactta tcagtggtgg gttggagctt gcaatgaact tacaatagca
     9121 aatttagatc ctatatctaa aacaccagag tataaatatt gtgctgttaa acttgaaaaa
     9181 cttgaagacc aagaattagc agaaaaatgt gtaagagaag agtatcaaag tttgaaagac
     9241 aaaatgacag ctacaaatat ctaatgattt attagattta agatttataa aaggcagaaa
     9301 ggctaatatt ttatgaacaa tatatactca aaaactaaat tacaagaaaa tagtaaggat
     9361 aaagtttttg aaaatagtta tagtctagtg gactacaaat atttaaattc ttttaatgta
     9421 gaagtaagca aaataaatga acatgaagta tcaatagata atgaagaaat aattgcagaa
     9481 tatccactta gttttatttt aaatgataaa tatataaata catttttatg taccccatat
     9541 aatttagagg aacttatagt aggattcttg aaaacaaaag gatacataga aaacaaaaaa
     9601 agcttattaa aatttgagat aaatgagagt gaacgttttg caaaagtaac tattcaagag
     9661 aaacaaaaaa atcaagatga gcaaattata tttttaaatt cactagacta tataacatgt
     9721 gctcctgtaa agagcaatat aaaaataaat tatgatacga tttacagtat aatggataga
     9781 aatttaaatt catcagaatt atttaaaaat actggtgggg tacatagcgt atctgtattt
     9841 aattttgata aaaaaatagt cacttgtgaa gatgtagcaa ggcataatgc aatggataag
     9901 gctattggat tttgtgtttt aaatgacata acattaaaag ataaaattgt ggctgtaagt
     9961 ggaagaatat cattggaaat gatattaaaa gcagctatga ctcaaattcc tattgtagta
    10021 tcaaaatcag caccgactaa tttatcaata gagctttcta aaaaattaaa tataacgctt
    10081 attggatttg taagaggaag acggatgaat atttatgcaa atccacagag agttatcaaa
    10141 aagtgagaat gaatatatgt gtactaaaaa agaagagcaa agatattcaa agtgtgaaat
    10201 ctgtggagta ttaattgatg atactgataa ggtgtataat aacacataca atttgtgcaa
    10261 aaaatgtaaa cgaaatataa gtactgagaa aatagtgaaa tatataaata tatatgatga
    10321 aagtaagtag acatatgatg aaaataacta gagggttgga aatatacaaa ttgaaaggtg
    10381 ggataatatg aaaaataaaa taggctcttt tgtaatagca gacccaaaca aatgtacagg
    10441 ttgtagagct tgtgaagtag cttgttttac aatgcataat cagcataata atgtgggtta
    10501 tacagttgga acagttgata tacctgttat acctagactt tacttagtaa aaggtgacaa
    10561 tttctgtatg ccaatacagt gtagacagtg tgaagatgct ccttgtttaa actcatgtcc
    10621 acaaaaagct atagttaaag aaaacaatat tatgtctgta aatgaagaaa agtgtatagg
    10681 atgtaaaact tgtctgctag catgtccttt tggagctata gacttacttc cacaatatga
    10741 agatggtaaa gaagtagaac aagtaatttt agatgaaaat aagaagatag cttataaatg
    10801 tgacttatgt aaagacaatg aaaaaatagc ttgtataagt gcatgtcctc aggaagcatt
    10861 aaaacttgtt actccaatag atgacaaaaa agctaagaat agacaagctg cacttagttt
    10921 attgttaaca aataaataaa ggatacaggg gtaggtgacg tttatgataa taaatataga
    10981 taaggattta tgtactggat gtagagaatg ttcaaaggtc tgtccagtaa atgctattga
    11041 aggtgaagag ggaaagccac aagagataaa tttagataga tgtgttatgt gcggtcaatg
    11101 tgttcaaact tgtaaatctt atgcttcagt tatagatgaa ggatttgagt ttttacaaga
    11161 gaaaaaacaa gaaagagaga taccagagtc tataaatgaa cctatatttg cagcatacaa
    11221 tgtatgtgat atagataagg tgaagaaagc tttaagtgac ccaaatgtat tcactatggt
    11281 ccaatgtgca ccagcagtta gagttgcttt aggagaagat ttcggatatt atttaggata
    11341 cttaggtgct gggaaaatgg cagcagcact tagagcttta aattttgata gagtctacga
    11401 tactaatttt gcagcagatt taactataat ggaagaaggt agtgaattaa ttaaaagagt
    11461 gacagaaggt ggaactcttc caatgtttac atcttgttgt ccagcttggg taaaattcat
    11521 ggaaaaaaat tatcctaaat taactaatca tttatcttct tgtaaatcac ctcagcagat
    11581 gggtggtgca atatttaaaa cttatggtgc agagataaat aatgtagatg catcaaagat
    11641 atttagtgtt tctataatgc catgtacatg taaaaaatat gaatgtgata gagaagaaat
    11701 ggattctagt ggatatagag atgtagatgt agtattaact actagagaac tagcttattt
    11761 aataaaagac atgggaattg attttaaaaa tttaagagaa gaaaaatttg atagtccttt
    11821 aggtagttat agtggagctg gaactatctt tggtgttact ggtggggtta tggaagctgc
    11881 tataagaaca ggatatgagc ttataactgg agaaagtatt cctgatgtag aagtaaaaca
    11941 agtaagagga gaaaatggtt ttagaaagtc aactattaaa ataggagact tagacttaag
    12001 agttggagta gtaagtggat taaaaaatgt aataccagtg cttgaagact tggaaaaagg
    12061 aaaattagat gtagatttta ttgaagttat gacttgtcct gttgggtgtg taagtggtgg
    12121 aggtcaacca aaaattttac tagaagaata taaggaattg gcttatgaaa atagaacaaa
    12181 agcaacatac gtgcatgatg aacatttagc aattagaaaa tctcatgaga atcctgatat
    12241 taaaaaactg tatgatgaat atctggtaga accattagga gaaaaatctc atcatctatt
    12301 acacactaca tattgtatag gaaaggaagt ggctaagtga tgaactattt tgttatagca
    12361 gaccctgata aatgcatagg atgtagaact tgtatgatag gatgtgtagt tgcacattca
    12421 gatgaagata ttttttacca aaatctagat gaaataaatt ttaatccaaa actttctgtc
    12481 ataaagacaa aagaagtaag tgctccaata cagtgtagac attgtgaaga tgctccttgt
    12541 gcaaaggcat gtccaaatgg tggtatagtt agggttggga acacaataaa aataaatgaa
    12601 gaaaattgta taggatgtaa gacttgtatg ttagcatgtc ctataggtgc aatagacata
    12661 gttacattaa aagatgtaga tgaaggaaag ctttgcttta gagaaagaat gacagcaaat
    12721 aagtgtgatt tttgtataga atcgccagaa gggcctgcat gtgtaaatgt ttgtccaact
    12781 aaggcattta ctatagttaa ggatgaggat atagatagta aagttaaaaa taagagaaaa
    12841 ttagctatat tgtaatataa aaaagatgga gttttattta tgattaaaag ctccatcttt
    12901 tttattataa tatttatata tctaaaaatc aattattgca ttagtttatc taatctttct
    12961 ttattcaatt ttttcattcc tatagaagtt ataataagta aagtataggc aattataact
    13021 gataaaatta taatagacca agaaaatctg tatccaaaat tatcaactaa caatccaaat
    13081 aatgttgaac ctaatgcaaa tcccactgca aaaattaatt gtacgattcc atatatgcta
    13141 ccaaactcct tttgaccaaa gaaagagcca gttaaataag caggaccaat catataagca
    13201 aatacagaaa gacctttcaa aatggcaaat acaaatgcaa aagtagaaga tttatctacc
    13261 aatagtaatg ataaagttgc tataagaacc aatacaaaag atataataag tggctttgtt
    13321 tgaccgaatt tatcaaacaa taatcctcca aataaattac caatgagagc aaataaagca
    13381 aatgtggagc ctactgttcc tattagaata ggatttagat aatttctaag ataagttgga
    13441 tattgtacgg ataatgatga aacatatata ccaacaaata taaatccaat agtcataatc
    13501 caaaaatatt taattttttt agcttctttt aatgtataac cccatactga aggagaaatt
    13561 gtatcagatg ctttctcttt tttaccttta gattttacct tttcattaat atctttaggc
    13621 atcctgagta tgaataatat tacaggtata ccaactatta atgctataat tccaaatatt
    13681 agataagaac gactgtatcc ttgagttgct aataagcgaa ctgcaagtgg ttgtagaaat
    13741 atactaccaa tagaaccacc agcaaaagca aagcccaaag cttttccttt gctttcttca
    13801 tcaaaccaag cacttattaa aacaggtatt cctatagcag atataattgt agcgccaatt
    13861 tgaacaatac atgatacagt gtaaaattgc catagttcag tacatataga aaaagtagca
    13921 aaagcaatac tagaaattat acaacctaat aaatatagta tcttaatatt gactttagaa
    13981 aataatacac ctataaaagg tgatgcaatt gctgaggcaa tagtaccaat agtgaatatt
    14041 agagaaaaag aagctaaact aaagccttct ccttctacaa cataacttat aaattgaggc
    14101 tgtatacttg ctacaatact aaatggaaca gcttgtatca acatacaacc aagtactata
    14161 acccaagcat ttgaaaagtt tttaatgcta aatttattgt ttagtaatgt attcatggta
    14221 tctctccttt aggttatgat ttgtagatat taatctaatt attctaattt tatatattag
    14281 ttttggatag cccaactatt taaatataat ttgggtatta atataaaaat taactataaa
    14341 aattgattat tttaaatgta tggggtaaat taaagtatat aaataaatga aaggatggat
    14401 aaaatgtcat ttgaaaaaat aaataataat aacctagata aaatacacaa caggaagtgt
    14461 attatgcttg taaactttaa taaaaaagaa ttatcattaa ttaaaaacgt aagtaacttt
    14521 gctggaataa gagaccatat catcttaagt gataaaaatg gagagaatat aataagagac
    14581 attttagatg gaaatatatc aaatgaatgt gaaaatggga ttaagagtaa gtctgtaata
    14641 tttaataaca ctccaagcac acaagtaaat gcatttttag aagggctaaa aaaagtgaga
    14701 ataaatagac cttttacagc tatggtaaca gagacatcta ttgattggac attgaataat
    14761 ttaatagaaa atttagttca agaacgtaat gctatgtctt taggaaaaac aattgaacat
    14821 aagtaaattt aagatatctt atagtaattt taactaatgt acattgagta tcatattata
    14881 ttttagaata taaaatttta aatgtgggta gatggaggag atttgagatg aaagttttat
    14941 ttacaataaa ttatggaaaa gaaaaatttg ataaaataag agaattaggc tatgatgtca
    15001 tacattactc tgaagatagt attgaaaata atgaggatgt aaatacagca gatgtactag
    15061 ttacatacaa tccatttgag agattagata tttctaagat gaaaaattta caatatatac
    15121 agacaactag tgtaggtata gaccaacttc caaaagaaga ggtattgaag agaaatatta
    15181 agatagctaa taatagaggc ggatatagtg tacctattgg tgaaagtatt gtattgtata
    15241 ttttagaaat atataaaaat agtgcaaaat tttttaaaca acaacaaaat aaagaatgga
    15301 agatggattt ttctgtatca gaattgtatg gacaaagagt tggattttta ggaacaggta
    15361 ctatatcttc agaagcagca aaaagattaa aagcttttgg tgtagaaata tggggtgtca
    15421 atactgatgg aagtagtaaa aaatactttg ataagtgttt tgctactgaa gatatgaata
    15481 ttgtatttga agaatgtgat atagtagttg tagcaatgcc ttctacaaaa agtacagatg
    15541 gaatagtaaa caaagatatg tttaaactta tgaaagatgg ttctgtattt ataaatgtag
    15601 gtagaggaaa tacagttaat cagaaagatt tagaagaatg tgtagagaaa tttagagggg
    15661 tagcattaga cgtatttgaa agtgaacctt taaacaagga caataagttg tgggagtgcg
    15721 aaaatgtcat agtaactcct cataattctt gggtgtcaga taaaaacaaa gagagaacat
    15781 ttaatatggt ttataataat ttgaaacact atataatgga gaataaacct gtagataatg
    15841 ttgtagacat atcaagaggc tattaaaaat aaaaaatttt gataaaaata aaaactctta
    15901 ttagtcgtat taaagatact aataagagtt ttttttgtaa taaatttaaa gcccaaaaag
    15961 tgatatacta aaaaatatat aaataggaga taattaatta tagggaaatt tcaaaataat
    16021 ttaattttaa aaatatgaag aggtgcaaaa agtgagcgta aaagatgaaa ttaataaact
    16081 tatagagaca ataaactacc ataatgaaag gtattataat caagatagcc cagaaatatc
    16141 agattatgaa tacgataagt taatgaagga gttaatttct ttagaagaag aacatccaga
    16201 actaaagaga attgattctc caacaaatag ggtaggaggt aagccattag ataagttcaa
    16261 tcaaattgta cataaaactc ctatgttgag tttatcaaat gctttttcag agcaagattt
    16321 gagagatttt gataagagag ttcaagaata tgtgggagaa aatgttgaat atgttgttga
    16381 atttaaaata gatggtcttt ctgtaggtct aacttataac aatggagagt ttgaaaaagg
    16441 agcaactaga ggagatggag ttgtaggaga agatatttct caaaacttaa tgacagtaaa
    16501 aagcatacct cttaagataa atgataaaaa agaactaata gttagaggag aagtatatat
    16561 atcaaaagat aattttagaa aagtaaatga acaacaagaa gaacaagaac agccattatt
    16621 tgctaatcca aggaatcttg cagctggttc attgagacaa ttagacccaa aactaacagc
    16681 aaagagacca ttagatatat ttgtatttaa tatggaaaat atagaagaat acgatttaga
    16741 aagtcacagt gaatcactag agtacttaga aaaactagga ttttcagtaa gtcagagtta
    16801 taaagtttgt aaaagtatag atgaagttat tgagcatatt gaatactgga cagataacag
    16861 gggtaattta gactacgaaa tagatggtat ggtaataaaa gtaaacaata taaaacaaag
    16921 agacgaaatg ggatatacag ccaaaagtcc tagatgggca attgcatata aattcccagc
    16981 agaaaagaag aaaactaaaa tagtagatat aatagtagaa gttgggagaa ctggtacaat
    17041 aacaccttca gcaattcttg aaccagtcag acttgcaggt actactgtaa gtagagctac
    17101 acttcacaat gaagattata taagagaaaa agatataaaa ataaatgata cagtattagt
    17161 tcaaaaagca ggggacataa tacctcaagt tttagaagtt ataaaagaag aacgtactgg
    17221 agatgaaata gattttaaaa tgccagaaaa gtgtccagtt tgttcagagc caacaataag
    17281 attagaagga gaagctgctg taaaatgtat caatatgtct tgcccagccc aaataagaag
    17341 gggaattata cactttgtgt caagggatgc aatgaatata gatggattgg gagaatctat
    17401 aattacactg ctattaaatg aaaaaattat tcaagatgta tctgatttat attatataaa
    17461 aaaggaagat gtagtcgacc tagagagaat gggcgaaaaa tctgctgaca atttaataaa
    17521 ttcaattgaa aaatcaaaag aaaatgattt atggagactt ataaatggat taggtattaa
    17581 atttatagga gttaaagcag ctaagatact tgcatataac tttaaggatt tagatgaagt
    17641 aatttctgca actagtgagc aactagaaga attagaggaa tttggaagta taatggcaaa
    17701 cagcgttgtc gagtttttta aggaagacaa aaatatgaac gttataaaca agttaaaaaa
    17761 tgtaggagta aatacaaaat cattggataa tacaggtgaa aaagtagaaa aaatatttga
    17821 agggatgaaa atcgtattga ctggtacact tcctacatta aaaagaaatg atgcaaaaga
    17881 tatgatagaa aaaagaggtg gaaaagctac atctagtgtt agtaaatcga caacatttgt
    17941 tttggcaggt gaagaagctg gttctaaact tactaaagca aatgatttag gaattaaagt
    18001 tatagatgaa gagagatttt tagaattatt aaagttatca tcgaaagatg aagtaaaagc
    18061 tgtattagag agttaaaaag tcgcttaatt tagtagaaaa ctactataat taatgataga
    18121 atagttcagt aagctaataa gattacgaaa aatgacaata attaatattt gacaaaatta
    18181 aaaattataa aaataaattt ataaaaaata aaaaatatga gaaaggatat aaaaaatgac
    18241 cagaagttat gatagaagta acgaacagat aagacctgta aagataacta gagattatac
    18301 aaagtatgca gaaggttctg ttcttataga aatgggagag acaaaggtta tatgcactgc
    18361 atcagtggaa gaaaaagttc ctcctttttt aagaaatagt ggtactggat ggataaatgc
    18421 tgaatattct atgctaccaa gagcaacaca acaaagaaag ataagagatg catctagggg
    18481 aaaaattgat ggaagaacac aagaaataca gaggttaata ggaagagcaa taaggtctgt
    18541 tgtagacttg aataaattag gagaaagaac tatttgggta gattgtgatg tgattcaggc
    18601 agatggtgga actagaactg catctataac aggggcattt gtagcagttg cagaagcaat
    18661 atataaatta tataaagcaa agataataaa agagatgcca ataacaaatt ttgtgtctgc
    18721 aattagtgtt ggtattgtaa atggaataca catgctagat ttatgttatg aagaagattc
    18781 taaagcacag gtagatatga atataataat gactgataag tgtgagtttg tagaagtaca
    18841 ggggacagga gaagagaaac ctttttcgag agcagattta aatttattgt tagagttagg
    18901 tgaaaaaggg aataaagaat taataaggct tcaaagagaa gctttaggtg aaatatcaga
    18961 tgagatattg ggtatggatt atggtaatga agtagtgatt gctactaata atgctcataa
    19021 gctagaagaa ataggtgaaa tcttaaaaga ttttgaatat aaagtgtact ctttaaaaga
    19081 tgttgattta gcaggtatag agatagtaga agatggaaaa acatttgagc ataatgcgtt
    19141 gataaaagct agaacaatag caaaaaagac taaacttata gcaatatcag atgattctgg
    19201 cttagaagta gatgctttag gcaaaaaacc tggtgtttat tcagctagat atgcaggaga
    19261 acatgcaaca gatgaagaaa atagaaaaaa actgttaaaa gctatgcaaa atataccaat
    19321 gagcaaaaga aatgcaagat tcgtctctgc aatagcagta gtttttcctg atggaaaaga
    19381 atttgtagta agaggaattt gtgaaggaat gataggattt gaagaaaaag gtaaaaatgg
    19441 ttttggatat gatagcttat ttatagtaaa aggatatgat aagacttttg gtgaaatacc
    19501 tagtgttata aaaaattcaa tcagtcatag agcaaacgca ctaaaactta tgaaacaaga
    19561 gtttataaaa agggtagtga agtaatgaaa ataggcatag taagcgacac acatatgatg
    19621 attaaaaata tggaaaaaac aataccatat ttgaaagaat gtgacctaat aattcatgca
    19681 ggagataact ttactgattc aagatatatt catagcatga ctaatgtagg tattattgca
    19741 gttaaaggta attgtgactt tgatgctgtt gaggaagaag ttgtctttga agtggcaaat
    19801 aaaaccatat tcttatgcca tggagataag tatggcgtaa aatatggaac tcatatgtta
    19861 gaaaaaaagg caactgaagt tgatgctgat atagttatat ttggccatac ccatacccca
    19921 tttagagaaa ttaaagatgg tgtactttac ataaatcctg gaagcacttc acttccaaga
    19981 ggagtatcgt ataaaagttt tgtcattatg gatatagaag aagatgacat aaaaattgaa
    20041 gaaatacgca tataaaggtg tatttatcta aaaaaatggc aaaaaacgct caaaaaatta
    20101 aattttatca aataagtaca acgatattaa tggaacactt gtaaataaaa aaacttatgt
    20161 gatataatta agtggttgaa ttataaatag aatgaattat aataccgtta tcgggtatta
    20221 cataaatatc actaggagga ttagaaggat gaaagcagaa ctaattaaaa aagagggtaa
    20281 taaagttact ttaaaaataa cagtagataa caataaattt gaagcagcag taaataaggc
    20341 ttataataaa agcagaaata aatacaatat acctggattt agaaaaggta aagctccaag
    20401 aattgttata gagactcaat acggaaaagg tatattctac aatgatgcta tagaaatatt
    20461 attcccagaa gtttatccag aagctatcaa ggaattagac atagacccaa tagacaatcc
    20521 agatttagat atagaagaaa taagtaaaga caatgggttg gttatggttc ttaatgtaga
    20581 agttaaacca gagtttgaat taggaaacta caagggtata gaaatagcta aggttgagaa
    20641 tactgtaagt gacgaaaatg ttgatgctaa actacaagaa atggttgaaa aaaatgccag
    20701 attagtaagt gtagaagaca aagctttaga agatggagat acagctataa tagacttcga
    20761 aggttttgaa aatggtgtag cttttgatgg tggtaaaggt gaaaattata acttagttat
    20821 aggttcaaac acatttatac caggatttga agaacaatta gtaggtaaaa aagctggtga
    20881 agaagtagaa gttaatgtta ctttcccaga agaatatcac tcacaagatt tagctggaaa
    20941 accagttgta tttaatgtaa aaataaatga tgtaaaagta aaagaattat ctgcacttga
    21001 tgatgaattt gctaaagata ctagtgaatt tgattcttta gatgaattaa aagcagatgt
    21061 aagagctaag ttagaagaag aagctaaaaa tagagcagat gcagaaacta gaaattcagt
    21121 agtagaaaaa gttgctgaaa atacagaaat agaaatacca gaagttatga tacaacatca
    21181 aatagataat atgttaaatg aacttaacta ccaattacaa tatcaaggat ttggattaca
    21241 acaattacta gagatgactg gtagaactat ggaagaatta agagaagaga gaaaagaaga
    21301 tgctaaaaaa ttagttaagt cttctttagt attagaagca ataactaaag ctgaaggaat
    21361 tgaagcaaca gaagaagaat ttaaggctga gttagaaaaa atggcttctg cttacaatat
    21421 ggaagtagaa aaaatagaag cttctttaag agatgccgac aaagaagata taaaaggtca
    21481 aataaaaata agaaaaacaa tagatttatt agttgataat gcaacaatag cttaataaaa
    21541 taaacgctaa ctaatatagt tgacgttatg aaatgtttag tatataatga tgtgcaaata
    21601 taatttagtt aaatgcgtat aataatttac agtagcttaa aaaaagctac tgtaaattat
    21661 atagaataaa gggggtatga aaccatggca ttagtacctg tagttgttga acaaacagga
    21721 agaggagaaa gatcttatga tattttttct agactattaa aagacagaat aatcttctta
    21781 ggagatcaag ttaacgatgc cacagcaggg cttatagtag cacagctatt atttttagaa
    21841 gctgaagacc cagacaaaga tatacattta tatataaatt ctccaggagg aagtataact
    21901 tctggaatgg ctatatatga tactatgcaa tacatcaaac cagatgtaag tactatatgt
    21961 ataggtatgg ctgcttctat gggagcattc ttgttggcag caggagcaaa aggaaaaaga
    22021 ctagcactac caaatagtga aataatgata catcaaccat taggtggagc tcaaggtcaa
    22081 gcaacagata tagaaattca tgcaaagaga attttaaaaa taaaagaaac tttaaatgag
    22141 atattatctg aaagaactgg tcagccatta gaaaaaatta aaatggatac tgaacgtgat
    22201 aacttcatga gtgcactaga agccaaagaa tatggtttaa tagatgaagt atttacaaag
    22261 agaccgtaga tagagagagg tgttaatatg tctaaatatg aagaaaaacg acaattaaag
    22321 tgttcattct gcggtaaaaa tcaagatcaa gtaagaagat taattgcagg tccaaatgta
    22381 tatatttgtg acgagtgtgt tgaactttgt gacgaaatta ttcaagaaga gatagaagat
    22441 actatagatg aggatactac atctttacca aaaccaaagg aaatgatgga gattctaaat
    22501 gattatgtaa taggtcaaga aaaagctaaa aaagctttat cagttgcggt ttataatcat
    22561 tataagagaa tatacagtaa aaaatctagt agtaaggata tagaaattca aaagagtaat
    22621 atacttttat taggacctac aggttctgga aagacattac ttgctcaaac tctagcaaga
    22681 actctaaatg taccttttgc aatggcagat gctacatcat tgactgaagc tggatatgtt
    22741 ggagaagacg ttgaaaatat acttttaaaa cttattcaag ctgctgattt tgatatagaa
    22801 aaagctgaaa gaggtataat atatatagat gagatagata agattgcaag aaaatctgaa
    22861 aacccatcta taactagaga tgttagtggt gaaggtgttc aacaagcact tctaaaaata
    22921 ttagaaggta ctgttgcaaa tgttccacca caaggaggaa gaaaacatcc tcatcaagaa
    22981 tttttaaaga tagatactac taatgtatta tttatattag gtggagcttt tgatggatta
    23041 gagaagataa ttcaaaaacg tggtggagat aaaacactag gttttggagc taaaatagaa
    23101 agtaaaaaag aattagattt agggaagcta tatgaaaaag tacttccaga ggatttatta
    23161 aaatatggta taataccaga gtttatagga agaataccag ttttagctac tttagagttg
    23221 ttggatgaag atgcattaat gcagatatta caggaaccta aaaatgctct agtaaaacag
    23281 tataaaaagt tattggagtt agatgatgtt gagctagaat ttgaagaagg tgctcttcgt
    23341 gcaatcgcta aaaaggccat agaaagaaat acaggagcta gaggacttag aagtatagtt
    23401 gaaagcgtaa tgatggaaac tatgtttgaa gtaccatcta gagataatat aaagaaggtt
    23461 atagtaacag agaaatctgt gaacgaagac tcagtaaatc ctatcatagt acttaaggat
    23521 caagaggaaa gtgcataaga tgatgaaaag gagctttaaa agctcctttt tttatgttaa
    23581 tatgctaaat ataaaatagg tatcattatt ataaaaatgt gagtaatata cctagaatac
    23641 caaagacgaa tacaattgca atggaaccaa tttttccttt atataaaaga taaaatgatg
    23701 ctataaatgc aattattcca tataaatccc aattaacttc tttggcagat gaaaaagctg
    23761 ctgataatat gaatcctaga gtaattggtc taagaactgt caataagttt tctacctttt
    23821 tattattttt aaatttagaa aataactttg atataatagt tagacctatt aatgcaaaaa
    23881 ttgtaacacc aatagttgcg gctagagaac caaagaagcc agcagtctta aatcctacaa
    23941 atgtagcact atttattgct acaggacctg gtgtagattg tgatatagca agtaaatcca
    24001 aaaactcttg attagttata aagtgatatt tagatataaa ttcttgctgg ataaatggta
    24061 gcatggcata accgccacca aaactaaatg cccctatttt aaggaatatt aagaagagta
    24121 gtattatttc tgacatagac tatatacacc tcctccaatt attaaataaa tagggcttat
    24181 attgaataca ccaacagata aaactgctat aattaatagt agtatgttag ttttagactt
    24241 tggtagtttc ttgaacatgc tcgtaaaaga atacaatata agagctatta caacaggcgc
    24301 aactccttta aaaaatccat ctaaaatttt agatgatgaa tttttaaagt atagatacga
    24361 taagactaag actatacaaa atgatggtaa agcagagcct aaggtacata taagaactcc
    24421 tggtatacca taaagtttat atcctaataa tacagatata ttaactgcca gtactccagg
    24481 ataactttga gctatggata aataatcaat aaattcttcc tgctctaaaa gtttttgttt
    24541 gtttacaagt tcatcttgta taagaggaac catagcgtat ccaccaccaa atgtaaacgc
    24601 accaattttt aaaaaggtta aaaacaattt caacataata gaaacctcca aaagataaat
    24661 aacatctaag tatactaata ttaatattat atcataataa aaagataata tagtatgata
    24721 gtattctaag aatttatcat taatgttgaa aattaacttg ttcaaatata taatattaaa
    24781 agcaaagcaa ttagactaga aaaggagtaa tgttgatgga acaaaattat actaaaatag
    24841 atcatgaatt accattaatt cctctaagag gattagctat atttccatat atgatattaa
    24901 attttgatat aggtagagag atatctctta aagctttaga ccaagctatg atggatgaag
    24961 aacttatatt tttaacttct cagaaagaag cagaggtaga tgagccagga gaagaggatt
    25021 tttatcatgt gggaacaatc tgtaaagtta agcaaatgat aaaattacca ggagatacag
    25081 ttcgtgtttt ggttgaaggt gtatcaaggg gtagagtaaa gaaaatagaa caagaagatg
    25141 ggtatttcag agcagtaata gaagaaatag tatttgatag tgataatttg gatagtgaaa
    25201 cagaagttga aattgaagca tttgtaagaa atgtatttga tgcctttgaa gagtatataa
    25261 atattggaaa tagggtatca ccagaaatat taatatcttt agctgatata gaagatgtag
    25321 atagatttat agatacgata gctgcaaaca tatatctaaa atcaagccaa aaacaagaaa
    25381 tattagaaga atttgatatt agaaaaagac ttgaacttat ttattctata ttattagagg
    25441 aaatagatat attaaaaata gaaaagaaaa taactttaag agtaaaaaaa cagatgaata
    25501 aggttcaaaa agaatattat cttagagaac aattaaaagc tatacagaaa gaattaggtg
    25561 aagaagaaga tataaactct gaagcagatg agtatagaga aaagcttaaa aaaataaaag
    25621 cacctaagac aactaaagag aaaatagaaa aagagataga taaattttca aaaatatctt
    25681 ctatgtctcc agatgtatct gtaagtagaa actatttaga tactatattc tcattgccat
    25741 ggaataaaga gacaaaagat aaattagata taactaaggc aaaagatata ttagatgaag
    25801 accattatgg attagaaaaa gttaaggaga gaatactaga gtatctagct ataagaactc
    25861 ttgctaagtc acttaaaggg cctataatat gtctagttgg tcctccagga acaggaaaga
    25921 catctatagt aaaatcaatc gccagagcat taaatagaaa atttgtaaga atatctttgg
    25981 gtggagttag agatgaagca gaaataagag gtcatagaag aacttatgta ggttcaattc
    26041 caggaagaat aattaatggg gtaaaagaag cccaaacaaa aaatcctgta ttcttatttg
    26101 atgagataga taagatggca gcagattata aaggtgaccc agcttcagca atgctagaag
    26161 tattagaccc agagcaaaat aaggattttg tggaccatta cttagaaata ccttttgatt
    26221 tatcaaaaat actatttgtt acaacagcaa atagtctaag taatatacca agaccattat
    26281 tagatagaat ggaagtaata gaagtatctg gatatataga agaagaaaaa ttaaatatag
    26341 caaaaaaata cctacttcca aaacaaatta aagaacatgc tttaaaagaa aactttataa
    26401 aaatagatga tgaaacttta agaagtataa tcaaccatta tacaagagaa gctggtgtaa
    26461 gaacactaga aagaactatt ggtaagattt gtagaaaggt tgccaaaaaa tacgttgaag
    26521 acccaactct tgaagaggtt gttataaata agtctgacct agagacatat cttggaaaag
    26581 acatgtttaa gtatcaactt gcagaagtta accctcaaat aggattagta aatggtcttg
    26641 cgtggacaga agttggtgga gttacacttg aagtagaagt aaacgtactt aaaggtaaag
    26701 gcgaaattgt attaactgga aaattaggag atgttatgaa ggaatctgcg aagacaggta
    26761 tctcttatat aaggtcaata gtggacaagt ttgatataga ccctgaattt tataagacga
    26821 atgatattca tatacacatt ccagaaggtg ctgttccaaa agatggacca tcagctggta
    26881 taactatggc tcttgcagta atctcagcac ttactaaaag acctgtgcct ggaaatatag
    26941 caatgacagg agagataact ttaagaggta gagtcttagc agtaggagga gttaaagaaa
    27001 agttattagc agctcataga gctggtataa caaaagtatt aattcctaaa gaatgtgaag
    27061 ctgatttaga tgaaatacca gaaaatgtaa aagaaaaaat ggagtttgtg cttgtagaac
    27121 atatggatga agtattagaa caagctttat taaagagtgg tgaaaataat gaaaattaga
    27181 agttcagaga taactatgag tgctgtgaat aaatcacagt atccagcaga aggaatccca
    27241 gagatagcat tggctggtag gtcaaatgtt ggaaaatcat caataataaa tacattgctt
    27301 aacagaagaa actttgctag aaccagtcaa acaccaggaa aaactagaac tataaatttt
    27361 tatctaataa ataatgaatt ttattttgta gaccttccag gctatggata tgcaaagata
    27421 gctaagtctg aaaaagaaaa gtggggaggt ataatggaac gatatctaga aagtagacaa
    27481 gagttatgtt ctatattcct acttgtagat attagacatg agcctacagc agatgataag
    27541 cttatgtatg aatggataaa gcactttgga tataactgtg tagttattgc aactaaggca
    27601 gataaaatat ctagaggaca atatcaaaaa cacataagta taataagaaa aaaattgcaa
    27661 atggaaagta gtgaaaaagt aattcctgtt tcttcactaa aaaagactgg tgttgaagaa
    27721 ttatgggaag agattgtaaa tcaatacaat caacatggat atgaaataac agtagattaa
    27781 atttaaaatg ttaaatattg acataagatt tatttgaata ataaatgtaa taatagttag
    27841 ttttaattat attaatatta aagtaaaata taggatgcct taaaatagtt ttaatgaact
    27901 tttttttggc atccttattg tttataaaca ataaatatca gtaattttgc taaatactaa
    27961 taaattttag taaggtatgg gaaaattagt ataaaatagt taaactaaaa aattattgat
    28021 ttatgtagtc agtaataaat agcatttaag aaatagattt atctgttttc ctcataaaga
    28081 aaacagttag tataagccct acaaaacata ctgatgctgc aatccaataa gactgattgc
    28141 tagctataac ataggctgat gtgtgacttt taaacttttg attgtatgta agactttgtg
    28201 caaaaaaact actaaaaaat gctgttccaa gagcaaatga taaattacga atagttgcaa
    28261 gaaaactgcc tccgtacccc tgtttttgag gaggaacatc tgctaaaata ctactattgt
    28321 ttggtgaact aaatgctcct agcccaagtc caaaaataac taaacataca attaaaaggt
    28381 acaatggaga atctgctttt aaaagtgcca ttccaactag tgagatggtc ataaatgaaa
    28441 aagatgctgt tagaatgttt tttgcaccgt gtttatcaga aagtgaacct gcaataggtg
    28501 ctataagtac cataataagt ggatgtacag tcattacata accagccatc ataggagata
    28561 aattttttaa ttgttctaaa taaaatggaa gtagaaaaga tactgccatt tgaggaaaat
    28621 aacaagctac tccaatgata ttgcctacag taaagtttct atttttaaat agagggaggt
    28681 caataaagct ttgttcaaat tttacttctc ttaatgccaa caataagaag attataagtg
    28741 ctgttatacc taaatacaat cttgtattac ccttctcatt cattgcaagt aatataagcg
    28801 tagtgcaaga tgcgaaaagt agaagacctg gaatatcaaa gctcttgttt tgttcatcca
    28861 aaacaggttt aggcagaaat ttaatgccaa gaaagactgc tataatacca atagggatat
    28921 tgataataaa aatagaaggc catccaaaat gttctaaaat tatacctcca attacaggac
    28981 cactcatatt tcctatacca actacagctc cagtgattcc tatagctttt cctttttcat
    29041 ttgctggaaa tgcattggaa actataccaa gcccagtagc cattaaaata cttgctccaa
    29101 ctgcctgaaa gattcttgaa gaaattaatg ttgataaatt gtttgacata ctacacagaa
    29161 aagaacctat tgtaaatata aaaaatccta atgtgtatag acgatttgaa cctactttat
    29221 cgccaagttt tccgaaaaat aacatagtag ctgttacaac aagcatataa gctgttacaa
    29281 cccactgaat ttgtgacatg ttaattttaa agtcagcagc aattgtagga tttgcgatat
    29341 ttagtatact tccatcaaga gtagacataa atacaccaat acagatgata atcaatacta
    29401 tccatttttg tttcatgata acactccaat cttaatagat tttataagta cgatataatt
    29461 gtaaaataaa caacaaaaaa agcacctgta tattaggtac cttgaatgtg tctaatttca
    29521 tgaataaata cggtagttga aggatttgta tagattactc atgtaaaatt acataaaggt
    29581 actcatatac tattttcttc taccatccag attttactgt cggctttgga atatgaccaa
    29641 attatgccat aaggctcgcg ggcttttacc gccgataggt aatttcaccc gtttatgaag
    29701 aaaaacagat gtattttgta atattatttt aacataaaat tataatggaa acaattggat
    29761 ttaagtaata tccagctgta ttacaataca aatatttcaa tatggacact taaattgtat
    29821 ttatagatag aataaggcac agtatgagaa tctataataa ttagataaaa ttaacctgtt
    29881 attagtatta gatggcaagt tttaaatttg tcaaaatcag aaagctgtaa taaaattctt
    29941 ataatatgaa tatcttaatt aagagatatt gtaaattatg aggaggcgct aatatggaag
    30001 gaaaggtaaa gaaggcattc ccgggtggaa atacatcaca aggctcatat tctttttttg
    30061 actatataat tcctgaaaat gtaaaaagag ttttttgttt aaagggtgga cctggagtag
    30121 gaaaatcctc acttatgaaa aaaatttata aagagttttt gcatagagga tatgatattg
    30181 atttatatca ttgtccttct gacccaagtt cgttggatgg tttagttata aagaaattag
    30241 gcgtggtact tatggatgca actgccccac atactatgga cccaaagcta cctggagctt
    30301 tggatgagat aattaattta ggtgattact ggaatacaga agaattagaa aagaataaac
    30361 aagaaatatc agtttatgat aaagatatta gtaattcatt taaaagagca tttaagtttt
    30421 taaaatcagc agaaccaatc tttagagaca tagaagaaaa atattctgat tgtatggatt
    30481 atggcaaagt aaatttggtc acagaagaat ttataaaaag gttatttgat ggagttaaaa
    30541 gcacaggaaa tctaaaaaga gaaaagcatt tatttggttc agctataact cctgtagggt
    30601 gtttagatta tacagaaaac attttaaaag atgtaaagaa agtctattat ttagatggag
    30661 aaataggaac tgggaaaact acattattaa acaaagtata taagaaagct attgaaaaag
    30721 gtttaaatgt agaagtttat cattatccac ttattccaga gaaaattgaa actgtactat
    30781 taactgattt agatataggc ataactataa gtacgacttt taaagaagaa gatacaattg
    30841 atttaaatca gtttttaaat agagaaaaac tacttaaata tgaagaagat attaaatttg
    30901 atgaaaaagt tttagatgat ttaatttgtt gggcagtact aaatcttaag agagccaagt
    30961 ctaaacatga tattatagaa agttactata gtcctaatat gagatttgat gaagtaggga
    31021 aattaagaga taaattaatt aaaagaattt taaaatatga ggaaaactta taaacgtata
    31081 tgtttaaaat taatgttaat aatgatataa tttttagtaa attggtgtag ctttgaggct
    31141 atgccatttt actttttttt gctaaaaaat gatattaata atctccaaag caattatttg
    31201 tttttcataa ttattatttc ctttcctaat ttatgttttt ttataatatc aaaacaatgt
    31261 ttattgaaag tttatataat tttaagaaca tctaaaaatg catacttgat attggtaaat
    31321 tagtgatagg agttcaatgt atgatattat atactaaaga tgaattttaa tatataactt
    31381 tgatataata agcatatgta aatgtttaat atttaattaa agaaaattat catataaaaa
    31441 taaatttgta aggagcaata cagtatgaaa aaacaatatg tgtttataat actagcaagt
    31501 actattttag gtatacttgt tggaaccata tttagagatt atagcaatga agaaacagtt
    31561 tatacaagca aagacaagtc aataagaaaa gaaataaaaa tgacaagaaa atctataaaa
    31621 gaattaacca aagagaaaga caagttagat aaagaaatta attcaatgaa acaaaatcaa
    31681 aaaaattctg aagaaatatc agaaatagaa acacttagag atagtctatc atattcagat
    31741 gtaaagggta gtgggataat attaagtata gatgctatag atgaaaaaac agggaatata
    31801 gctaatagta tagactataa taagattctt gtaaaagtta taaatgaatt aaaagtaaac
    31861 ggaggaaaat acatagaaat aaatggtcaa agaattaatc aatattcaga agttgtactt
    31921 gcaggaagcc atataaatat aaatggagtt cctgtagcac aaccatatga aataaagtgc
    31981 attggagata aggagaaact atctaattat gtagataaag gtagtaaata tttagatagc
    32041 atacaaacca attatccaat gaaaataagc ataaaaactg aagaaaatat ctctatgaaa
    32101 aaaatgaata tacctaataa attaaagtat ataaagggtg attaagatta tattagagga
    32161 aagcagtttg ccaatgttaa taccagaaaa acttgcaaaa aaatataatg tatttccagt
    32221 tgagtttcaa gatgataatt tagttgtaga aatagaagat gaggatattt atgctctaca
    32281 ggacttgaga ttagctacag gtaaagaaat tatattgaag aaggagaaaa aagaacttat
    32341 aagtgaaaag attgaaaaat attataatag ctttgaactt gatgaggatt atgcaaaaaa
    32401 attatttgaa aatcttttag aaaaagctat aaaagaaaac gcaagtgata tacatataga
    32461 accatttaaa gaacatttaa tagtgagaat gagggtagat ggagaactaa aggaagtatc
    32521 aaagttttta gttgatgtct atccatcatt gtctacggtt ataaaattaa aggcatcaat
    32581 ggatataaca gaaaaaaggc ttcctcagga tggtagggtg gatataaagt taggtggaaa
    32641 tttaattgac attagagttt catcaatacc aactgtctat ggtgaaaaaa tagttttgag
    32701 gatacttaat agagatactt ttttaaaaga caaattagaa cttggttttt cagaggaagc
    32761 tataaaatct ataaatcgta taataaataa aagagcagga atattattag tgacaggtcc
    32821 tacaggtagt ggaaagacta caacagtata ttccttatta aatgatttaa aaggaataaa
    32881 taaaaatatt atgactatag aaaatcctgt agagtataag atggatggca taagtcagat
    32941 tcaagtaaat tcaaaagtag ggttgagctt tgatgtaggt cttagagcga tattaagaca
    33001 agacccagat ataataatgg tcggagaaat aagagatgca gaaacagcta agatagcagt
    33061 aagagcagct ataacaggtc atttagttat aagtacttta catactaatg atgcagtatc
    33121 ttccatagct agattactag aaatgcaaat accaccatat cttttaaatg cttctcttat
    33181 aggtgttata tctcaaaagt tggttagaaa agtctgcaat cattgtagcc atgagattat
    33241 gataaaagat aattttagtg gagatgtaaa tactaaggta gctgtagggt gtgaaaaatg
    33301 taatgataaa ggatactttg gaagaacagc aatttatgaa atcttagaaa ttaatgatga
    33361 tataaagaca tgtataagaa atatggagga ttctagtact attaaagatg tagcacaaaa
    33421 taatggaatg ataacatttg aagatagttg taaaagacta ataaatgaaa aaataacgac
    33481 attagaagag tgtttagtag taaatgaaat gatttaactg aattgtatag ttaaacttcg
    33541 tttggtcagt tgtctttatt taatctattt taattaagat gtttgagtat aaattatatt
    33601 taagtgaaat aaaattaaat gcattaaatt agaaaatatt aagtttattt aattgtaatt
    33661 aatataattt ggaaggtatt atagtacatg agattatcta aaaatatatt ttataattta
    33721 gaaaagtttt atttagtatt aaatagaatc agtgataaag aaattagagt aatttgcaaa
    33781 gagataggca tattgctgga atctggatgt gaaattacaa aaatatttga aatcattgag
    33841 agtcagtcaa gtaaaaaagc aaagaatttg cttagtatag tatcaaatca tattcaaaaa
    33901 ggtaactcaa tagcagaatc atttcaaata acaggtatat tttctaaatt ctttataagt
    33961 atgataaaag caggagaaac tagtggaaac ttagatataa taatgagtga tttatctaat
    34021 tattatgata aagaatacaa attaaaaatg aaaataataa ctatttcaat atatccaata
    34081 atacttataa tattatctat cttatcaatg ttgtttatat tcgtatttgt aatacctaat
    34141 tttcaggtgg tttttaccaa caatggaatt gaaccacctt taataactag agttttaatg
    34201 ggaatatcta cagtggttac aaataactta gcttatataa tatttagttt tatattgttt
    34261 tcaataggaa gcatttattt ctttataaca aatgataata taaaaaaatt gattaattcg
    34321 ttaaaattca aaataccttt tataaaaaaa attaatcagt tagtagctac aacaagattt
    34381 tcaagggcat tgtccgtttt aattagcagt ggaatacaaa ttgtggaagc tatagacatg
    34441 tcagctagtg ttatagacaa cgaatttatt tatgaaaggt taaagatttc caataattat
    34501 ataaaaaaag gaaatagcat aggtaaatct ttgaatttag caaatgtttt tccaaaatta
    34561 tttatttcaa tggcaaatac aggagaagaa agtgggaaac tagataaaag tctaaatgta
    34621 ataaataaat tttatgaaaa tgaattaaat aacaaaatag agcaaataat gaaatttata
    34681 gaacctatgc taattgtatt tatgggatta attattggta cttttatttt agcaatggtt
    34741 gttcctatgt ttgatgcaat tactaatttt tagagagaaa aataaatttt attagatgaa
    34801 acttttgaaa aggagagaat atttaattga taaatttgat aaataaaaaa cgaaaaggat
    34861 ttacacttgt tgaaatgatt gtagtagtaa ctattttagg cgttatatct agtatagcat
    34921 tagttaagta tagtaaggtt caagaaagtg ccaaattaaa tgcagactat acgaatgctg
    34981 ctaatatagt aaccgcagct agcatggcaa ttaatgatga tgaaaagaca atagactctc
    35041 taagtgtaga gacattgaag gaaaagggat acctaaatac tgttccagtt cctcagagta
    35101 catcaggtaa attcgaactt gtcataaatg atagcggaac agatataagc gtaaatataa
    35161 attctaagca attttatcca aaataatatt ttatattgat tgctgtaagt tttaaaatta
    35221 tttttattta agaaggagat ggctataatt aaaaaaattc taaacttaga aataaatgat
    35281 gattatataa ggcttgtatt tttaagaaaa agtcataaaa aagtttttat agataaatca
    35341 attataaaag agattaactt tgatataaaa aatgatagta attcgattga gaagattaaa
    35401 tatatagaag atgtagttag atttgtaaaa gaggagattg gtaaatcaaa aatattatat
    35461 aaaaatttat actttaacat tcaaattcag gatatagtca ttagaaatgt tgaagttcta
    35521 aatactaaga aaaaaagaga catattaagc atgattgaat ttgaaataac acaatacatt
    35581 ccaataaaca taaataacta ttcaataaaa tataaagtta taaattctaa aaatgataaa
    35641 ttgaatatac aagtaattct tttcccaaag agtattattc aattaattaa gaacatttca
    35701 gaaaatctaa atatgaatcc aaagactata aatataaatt ttgatatatt acaaaagctt
    35761 ataaacttaa atttaataga gaatttttca gaagaagggg tatttataga atgtaaaaaa
    35821 agtcagttaa ttataaatat aacaaaagat aaaaaaataa gagaaacata cttgctatca
    35881 aaagagggaa aatcatatga gttcataaaa agattattaa gggattttaa gtatgtttat
    35941 tactatggta tagaaaattg ttttattgaa gaatacttta aatatacaga taaatctatt
    36001 gaagtaaatc cattaagatt aaaagataaa gtaaaagtag aaaataattt tgatgcagat
    36061 ttagaagaca ataggataaa ttatataagt aatataggaa tgattattta agtgaaagtg
    36121 gataaaacga taaattttct taatggattt aaaaaaacta aaccatgtaa taaaatctat
    36181 aaaataataa tagtcttaat tttaacgtgt attttattta aatcatttat gtatgtgaaa
    36241 tctataaatg aacttgaaga ttatataagt caaaataagc tagtggataa atcaagacac
    36301 actttaccaa caacagataa aactagagag aaattttcta aaataaaact taatgaaata
    36361 aaaaaaatat atgaaacaat tggaataaat attattacta aaatatcgat agataaaaat
    36421 aagctagaga ttgaaggtgt gtgtagtgat ttagatgttt taaacaatct aaggaaaata
    36481 aagtacataa aatattatta catagacagt attgtaagag atggagaata ttatagattt
    36541 agaataaaaa gttagatggg tgattatttt tgagattaaa taatagagaa aaaaatctac
    36601 ttaaaatatt gttaatatta gttattatat tggcaagtga tatagcagta agttatttgg
    36661 aagaaaactt aactagtttg aagaatgaaa agcaaaatat aataaataaa ataaataatc
    36721 aagataatta tcaaataaag gataataagc atatagatga taaatatata gaaagtgttg
    36781 aatcaaataa aaatgaagaa ttaaaaaata attttaataa ctcattttta gataaaaaaa
    36841 caaataaaga tatagtgtta ttagtagaaa atgaactgag agatattgtg aatgtagaat
    36901 atattaatca agaaagtagt ttagataatc acaataaaaa acaagttaat ttagaaataa
    36961 aagttaatgg agaacttgca aatttgttga aacttgaagg tgtaatgaag aagttaagac
    37021 taaataataa acttgaaaag atagaaatta ataaattaaa agtcaataat tttgatgttc
    37081 aaaatattcc aaatgaatat aaacaagaaa aagacaatat tgttgaatgt aaaatgacag
    37141 ttagagtcat ataacaaaaa agaatagata aagtaaaaaa ctattatcaa ttaataaaat
    37201 tttatagagg aattagtttt acgataattc atttctacaa gagggattgt tgatgatttt
    37261 atcgaaatcc taattaaagt taatatattg taccaaaata taagtgaatt taatagaata
    37321 tttacttata ctatagattg attaataaaa aaatggtata ataacaaatt gtactttaat
    37381 ttaataataa gggagtggaa gtcatattgt tagggagaaa tgagctatgc ccttgtggaa
    37441 gtggcaaaaa atacaagaaa tgttgtttaa ataaagatat tgtttcagaa agagcatgca
    37501 gaaaaactga actttcacaa aaacaatata cagatttata ttcaaagtta tatgattatt
    37561 caagacagga taagtttaaa gaagaatgtg aaaaagctaa agaaatgttt tatataatgc
    37621 aaaatgatag tgtaaatgaa aaatttgaaa gattttttaa tacttattta atacaagacc
    37681 acataatgga aaataaaaag gttatgacag ttggttttct tgaagaaaat ggtgcaaacc
    37741 taagtcaatc agaagtatca atattaaaaa gtatgtttga atcatacgta agtatttatg
    37801 aggtaaaaga agtttctgct ggaaaaatag tattaaagga ctgtttaagt ggaaatgaac
    37861 tttgtacaga agatgtaaaa cttctgagaa gttttaaaat cggaagttgt atgatagcta
    37921 gaatagttga tattgaagga actaacatat tgatagatat aactataagt atatcaaatg
    37981 aggttaaaga tataatttta aaggatatta tgaatctatt caatcaatat caagatgtat
    38041 acaaagatat gaagtcgttc ttaatttatc atacacatat actatacaag tatatacaac
    38101 agcttttaga cccaagtata gctgaatatt taaagagaga aagagataaa aacgatacaa
    38161 aagttaattc agaaaatgaa ttagctgaag aagaatgtaa agttttagat actcttaaag
    38221 aaaatgtaga aaaagaaaat atagatgcat gtatagagtt ttggaatgaa tataagaaaa
    38281 atcatgaaaa tataaaagga gctgaaaatg gttgggcagc tgctgttgaa tactatgtta
    38341 aaaaagaagc aggacaacct ataactcaag ttcaaatatc aaagaaatac gatataagct
    38401 ctagtacact aggaaagaga tacaaggatt taaaaatatc ttaattaatt ataaaaagct
    38461 ttaagtcata gataaagaat ctatagtata atatatgtat aatgtttaga aagaggtatt
    38521 aataatgagt cttagtttag gaaatgcaat tgctgtatca acgaacttta taaaatctaa
    38581 cccagctgta cctgtgtaca atggagaaga aatatatgca gttatttatc atatagaaat
    38641 aggatataat aatggtgtat cacctgatac caaacaatat ataataatag actataaaga
    38701 gtttgattta tctatggata aaaaaaatga actaataaat attgctaaag agtactgtaa
    38761 atctaaaggt attgtagaaa acgaaaacct tgaaataaac cttttagaca atgaagaagc
    38821 agagaacttc ttagaaaagg tgtttaataa tatgaaggtt ataagaggtc ttataactaa
    38881 aaaaggataa ataaatggac tatataggaa tagaaaatat aacaccttat gaaaatacat
    38941 atgaatttag tgtatatgaa tatgatgatg aaatcacctt aggtagtgaa aagttatatg
    39001 tatgtgaatt aagggttgta ttgattaaag ttaattctct gtatgttgaa agattgcata
    39061 aatcagttga agcaatggtc ttagtaaaaa acttgaaaaa agatttagat aaaacacttg
    39121 ttgtaaacaa aataaagaat tttgtgctag atgagatttg ggtagaaaat ctagtaaaag
    39181 agaatataga agttatattt gtagaaagct aggaataacc ctagcttttt tatattacaa
    39241 tctaagagag gaaaacaata tgttaagagt atctaatgta aaacttggaa ttgataaaga
    39301 tatatcttta ttaaaagatg ttatacttaa gaagctaaaa ataaaagaaa aaaacttagt
    39361 caaatatagt atatataaag aatctataga tgctagaaaa aaaggaaaaa tggaatttgt
    39421 ttattctgta gatgtagaag ttaaagatga aaataaaata cttaactcaa aaataaaaga
    39481 tgtaacaaaa ataaaggaaa tcagatacgt aaatgtacct gtgggtgata aaaaattaaa
    39541 gaataatcct ttggtaattg gtagtggacc agctggtttg tttgcaggtc tgcttttagc
    39601 tcaaatgggt tatgaaccaa tcatacttga aagaggtctt gatgtagaca gtagaaccaa
    39661 agatataagt gatttttgga caactagaaa attaaaaaat aattcaaacg ttcaatttgg
    39721 agaaggtgga gcaggaactt tttcggatgg taaacttaca actagaataa aagatatacg
    39781 atgtagaaaa gtattagaag agttagttca gtttggggca ccagatgaaa tattatactc
    39841 acataagcca catgttggaa cagatatatt aaaaaatgta gttaaaaata taagaaatga
    39901 aataataagt ttaggtggtc aagttagatt taattcaaaa gtaacagata tagttgtatc
    39961 aaatgattct atacaatctg taataataaa tgatactgaa aaaatagata cagatacaat
    40021 tatattagct gttggacata gtgcaagaga tacttatgaa atgttgtatt caagaggtat
    40081 aaaaatagtt caaaaacctt ttgcaatagg agctagagtt gaacatccac aagatttaat
    40141 aaataaagca cagtacaagg atttttataa tcaccctagg ttaggagctg cagattatag
    40201 acttatagag catgtttcaa atggaagaac agcatatact ttttgtatgt gtccaggagg
    40261 tagtgtcata gcatctgcat caaatgattt tgaaatagtt acaaatggta tgagtgaaca
    40321 tgcaagagat aaaataaatg caaatagtgc atttttagta aatgtaacgc cagaagattt
    40381 taagagtgat aatccactag caggtgttca tttccagcag aagtatgaaa aattagcttt
    40441 tgaattaggt ggaaaaaatt ataatgcacc agttcagctt gttggagatt ttataaacaa
    40501 taagacttct ttgaatttag gaagtgttga gccatcatat agaccaggat atacacttac
    40561 aaatttaagt gaatgtttgc ctagcttcgt aacagagact atgagagaag cgtttatatc
    40621 tcttgataaa aaattgaatg ggtttgcaat gtatgatgct gttttgacgg gagtagaaac
    40681 taggtcatca tcaccaataa gaatagtgag agatgaaaat actttagaat cagtgagtat
    40741 taagaatcta tatccatgtg gtgaaggtgc tggatatgca ggtggtatag ttacagcagc
    40801 agttgatggt ataaaatgtg cagaaaaaat aatacaaaaa tacaccaata atacaaaaat
    40861 ttaacataaa cttaatgatt ttttaatatt taacatgaat ttaacattat cactcctata
    40921 atttgataaa atacaatagg ttataaaatt tttagataca gtaatctctt tacaaggagg
    40981 aaataatttg aacattataa taagtctaat gggtggatta ggtctgttct tatatggtat
    41041 gaaccttatg ggagaaggtc ttcaaaaatc tgctgggaca aagctaaaaa agataataaa
    41101 actacttact agtaacctat ttatgggagt tttggtaggt acaggagtta cagcagtaat
    41161 acaaagttca agtgctacta ctgtaatggt tgttggtttc gtaaatgcag gaataatgac
    41221 tttaaaacag gcaataggag ttataatggg tgctaatata gggactactg ttacagccca
    41281 attggtatct ttcgacttaa ctggtatggc cccagtagca ttaggtgtag gtattatact
    41341 ttatttattt ggaaataagc ctagaataaa aaatatagcc gaaatactta taggttttgg
    41401 tatattattt acaggtatgg attttatgaa gatggcagtg gagccactaa gagactatca
    41461 aggatttact gacttacttg ttacatttgg tagatatcca ctacttggat tattattggg
    41521 atttgggatt acagcaataa tacaaagttc aagtgcatct atgggtatgt tggttgcact
    41581 ggcagcagaa ggtctagtgc cactatcagc agcactgcca attctttatg gccaaaatat
    41641 tggtacatgt gtaacttcac tgttatcaag tattggtgct aacaaaaatg cacgaagagc
    41701 agctatgatg catctaatct tcaatgtttt aggaacggtg atattcttaa tattcttgaa
    41761 taaaccagta gtaagcatgg ttacaagttg ggatccaagc aatgttgcaa gacaaatagc
    41821 caatacacat acattgttta atataatatc tgtattgata cttttaccat ttacaagtct
    41881 tattataaaa ctagctataa agcttgttcc tgatagagca ggtgatatag atgaagatga
    41941 aactaaaact attaaatata tagatgatag aatgatagaa actccttcta tagctcttgc
    42001 aaatacgata aaagaagcac ttcgtatggg tgagaaggcg aaggaaagcc taaatgcatc
    42061 tatggaagct ttagtagaac attcaactga aaaaatagat aagacatata gacgagagag
    42121 attaatcaat gatttacaga aagctatttt aaattattta cttaaacttt caaaagcagc
    42181 tttagatgat gattcaagag aagttgtgga tactttgttt aatacagtca atgatataga
    42241 aagaattggt gaccacgcta aaaatatagc tgaattatca caagttgcaa tagattcaaa
    42301 tatttatttc tcagaagaag gacaatctga acttgatgtt atgtataata gagttgtttc
    42361 agcatatact tatgcattag aatcaatgag gagcgataat gttgatttag cttgcaaggt
    42421 tataaaaatt gaagaacaag ttgatataat ggagaaatct tgtagagcta atcacatgta
    42481 tagattgaat aataatctat gtagtataga aaatggagtt atttatttag atgtaataag
    42541 taatttagag agaatatctg accatgctgt caatatagca caacaagtta tagcgaagag
    42601 attaggaaat gattaaaaat aattaattta aaataatata aaatttaaaa tagaggataa
    42661 ataaaagaga aactttaaat gatacgttta gagtttcttt ttttgtaaat ttatttgact
    42721 aaaattttta aatttataca tagttatagt atttagaaga atcgatttcc tgataataaa
    42781 tgatgagata tgtatgattt tgtggcaaac ttttaaaaaa aacacctata ttttgtgtta
    42841 taatctcttt aaaacgaata tgctgaaaag agggatttaa atgggaaaaa taaattttga
    42901 ttatagtaaa gctacagagt ttttttgtca aaacgaaata gatgtcatgc aaccatatgt
    42961 agatgttgcg cacgacatgt tacataataa aactggttta ggaaatactt ttttaggatg
    43021 gatagacctt cctaaaaatt atgataaaga agagtttgac agaataaaaa aatctgctga
    43081 taagataaaa tcagactcag atgttctttt ggtaataggt ataggaggct cttatttagg
    43141 ttcgagagct gctatagata tggtaagtca ttcttttagg aatggactta aaaaagagca
    43201 aagaaaggct ccagaagtat attttgtagg gcataatata agctcaacat atattatgga
    43261 tttattagat ataattgaag gaaaggatat ttctgtaaat gtaatatcta aatcaggaac
    43321 tactacagaa ccagctctgg cttttagaat atttaaagat tacttagaga aaaagtatgg
    43381 taaggaagaa gcaagaaaga gaatttatgc aacaacagat gcaagtaaag gtgcacttag
    43441 acagttagct acagaagaag gatatgagac ttttgtaata ccagatgatg taggtggtcg
    43501 tttttctgta ttgacagcag ttggattact tcctattgct gctgctggac ttgatattga
    43561 tgctatgatg aaaggcgcaa atgatgctag agaagcattt caaaatcctg acttaaaaag
    43621 taatgatagt tatagatatg ctgttgctag aacaatttta catagaaaag gaaaagatgt
    43681 agagttatta gtgaactatg aacctcaact acattatgta agtgagtggt ggaaacagct
    43741 ttatggagaa agtgaaggaa aagaaaataa aggtttattc cctgcttctg tagatttctc
    43801 aacagattta cattctatgg gtcaatacat acaagatgga aaaaggcttt tatttgagac
    43861 agtacttaat gttgaaaact gtaagagaaa tataactata agttcagagg aagtagactt
    43921 agatggttta aattatttag caggtaagac tgtagatttt gtaaatcata aagcttttga
    43981 aggaactcta ttggctcata cagatggaaa agtacctaat ttagtaataa atatacctca
    44041 actagatgag tataattttg gatatttagt ttatttcttt gagaaggctt gtggaataag
    44101 tggatattta ttgggagtta atccatttga ccaaccagga gtagaagctt acaagaaaaa
    44161 tatgtttgct ttactaggca aaccaggtta tgaaaaagaa aaggaagagc tagaaaaaag
    44221 acttaaataa ttttaaatag ttaaggttta attaagatta aaataatata atgaaatcat
    44281 caaaaaggag tttactcaaa atagatgatt tcattttttt tatgaaaaat tgggaaaaat
    44341 atattttgat aatttatatt atattaagat agggagtggt atgagatgtc aagaagaaag
    44401 aaagggataa gtttagtaat attagttgct ataataagtt caatacttag tagttttcta
    44461 acaataattt tagttaagga caatttagta agcaaatcta caggaagttc tacgccgata
    44521 gttgtaaatg atgatggtaa gagtcaaaat atttatcaag cagttgcaga aaaagctacg
    44581 ccatctgttg taggtattac aacaactagt gttgatacta gtaatatgtt tgcaatacca
    44641 actgaaacac aaggtgtagg aactggaatc atagttgatt caaatggata tatacttact
    44701 aattctcatg ttatatcaga tggtcaagct acaagtgtaa atgtattatt caatgatggt
    44761 tcaacaactt caggtaaagt agtatggttt gaccaacaac ttgatttagc tatagttaag
    44821 gttgataaaa ctggactaac accagcagaa tttgctgata gtgacaaagt aaaagttgga
    44881 gatatatcaa tagcaatagg taatcctctt ggattagatt ttcaaaaaac agttactcaa
    44941 gggataataa gtggattaga tagaactata caaacagaaa aaactaatat gactggttta
    45001 ttacaaactg atgcaagtat aaatgcagga aatagtggag gaccattgtt aaatcaaaaa
    45061 ggtcaagtaa ttggtataaa cacagcaaaa gcttctcaag cagaaggatt aggatttgca
    45121 ataccaatca atacagctaa gagtatagtt gaagaagtaa tcaaaaatgg aaaatatgaa
    45181 aaagtaactt taggcataaa aggtacagat gtatcaaatt atgaagcagc aacaggtaca
    45241 aaattaagta ctgataaagg tgtttatgtt gcagaagtaa tatctggttc atcagcagaa
    45301 aaagctggag taaaggtagg ggatattata actaaagtag gagatacaga tataacaggt
    45361 atgaatgatt taaataaaaa gttgtatact ttctcaaaag gtgctagtac aaaaataaca
    45421 gtaaatagag gcggaaaagc agtaacaata aatgtgaatt tctaaatatg accaaaaaga
    45481 tagagactac atggtaaagt gtagtctcta tttttattta atttttatag tatatacatc
    45541 taaattaatt aaaatgatgt attgtttata gtttattgct atattttctt ataaaaataa
    45601 atttaataaa tat
//
LOCUS       DAMJWS010000023        45316 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMJWS010000023 DAMJWS010000000
VERSION     DAMJWS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.023"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            182..850
                     /locus_tag="PC399_001351"
     CDS             182..850
                     /locus_tag="PC399_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592224.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            852..1337
                     /locus_tag="PC399_001352"
     CDS             852..1337
                     /locus_tag="PC399_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2592225.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1398..2519
                     /locus_tag="PC399_001353"
     CDS             1398..2519
                     /locus_tag="PC399_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF2592226.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2800..3990
                     /locus_tag="PC399_001354"
     CDS             2800..3990
                     /locus_tag="PC399_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2592227.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4124..4379
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4438..5439
                     /locus_tag="PC399_001355"
     CDS             4438..5439
                     /locus_tag="PC399_001355"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF2592228.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5543..5932
                     /locus_tag="PC399_001356"
     CDS             5543..5932
                     /locus_tag="PC399_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF2592229.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6093..7301
                     /locus_tag="PC399_001357"
     CDS             6093..7301
                     /locus_tag="PC399_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2592230.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7387..8463)
                     /locus_tag="PC399_001358"
     CDS             complement(7387..8463)
                     /locus_tag="PC399_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2592231.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8692..9219
                     /gene="hpt"
                     /locus_tag="PC399_001359"
     CDS             8692..9219
                     /gene="hpt"
                     /locus_tag="PC399_001359"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2592232.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9308..10240
                     /locus_tag="PC399_001360"
     CDS             9308..10240
                     /locus_tag="PC399_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF2592233.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10302..11528
                     /locus_tag="PC399_001361"
     CDS             10302..11528
                     /locus_tag="PC399_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2592234.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11612..11833
                     /locus_tag="PC399_001362"
     CDS             11612..11833
                     /locus_tag="PC399_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2592235.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            12022..12192
                     /locus_tag="PC399_001363"
     CDS             12022..12192
                     /locus_tag="PC399_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592236.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12285..12746)
                     /locus_tag="PC399_001364"
     CDS             complement(12285..12746)
                     /locus_tag="PC399_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592237.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            12929..13396
                     /locus_tag="PC399_001365"
     CDS             12929..13396
                     /locus_tag="PC399_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592238.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13439..15202
                     /locus_tag="PC399_001366"
     CDS             13439..15202
                     /locus_tag="PC399_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF2592239.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15210..15818
                     /locus_tag="PC399_001367"
     CDS             15210..15818
                     /locus_tag="PC399_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2592240.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15890..16459
                     /locus_tag="PC399_001368"
     CDS             15890..16459
                     /locus_tag="PC399_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2592241.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            16977..20516
                     /gene="nifJ"
                     /locus_tag="PC399_001369"
     CDS             16977..20516
                     /gene="nifJ"
                     /locus_tag="PC399_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF2592242.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(20908..21804)
                     /locus_tag="PC399_001370"
     CDS             complement(20908..21804)
                     /locus_tag="PC399_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF2592243.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PGNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPEDNNNSDSTNKPD
                     DNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYF
                     SHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFS
                     KIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(22300..23634)
                     /locus_tag="PC399_001371"
     CDS             complement(22300..23634)
                     /locus_tag="PC399_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2592244.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            complement(23696..24472)
                     /locus_tag="PC399_001372"
     CDS             complement(23696..24472)
                     /locus_tag="PC399_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2592245.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            complement(24577..25242)
                     /locus_tag="PC399_001373"
     CDS             complement(24577..25242)
                     /locus_tag="PC399_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF2592246.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            complement(25242..25898)
                     /locus_tag="PC399_001374"
     CDS             complement(25242..25898)
                     /locus_tag="PC399_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF2592247.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIIEGGNL
                     "
     gene            complement(25907..27085)
                     /locus_tag="PC399_001375"
     CDS             complement(25907..27085)
                     /locus_tag="PC399_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2592248.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTITVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQSKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            27440..28342
                     /locus_tag="PC399_001376"
     CDS             27440..28342
                     /locus_tag="PC399_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2592249.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            complement(28558..29370)
                     /locus_tag="PC399_001377"
     CDS             complement(28558..29370)
                     /locus_tag="PC399_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2592250.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLIDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(29373..30332)
                     /locus_tag="PC399_001378"
     CDS             complement(29373..30332)
                     /locus_tag="PC399_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2592251.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            complement(30438..31997)
                     /locus_tag="PC399_001379"
     CDS             complement(30438..31997)
                     /locus_tag="PC399_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2592252.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            32216..33193
                     /locus_tag="PC399_001380"
     CDS             32216..33193
                     /locus_tag="PC399_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592253.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            33194..34165
                     /locus_tag="PC399_001381"
     CDS             33194..34165
                     /locus_tag="PC399_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF2592254.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKKIKSVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            34317..36359
                     /locus_tag="PC399_001382"
     CDS             34317..36359
                     /locus_tag="PC399_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDF2592255.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNIMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVIKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATILLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            36620..37462
                     /locus_tag="PC399_001383"
     CDS             36620..37462
                     /locus_tag="PC399_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2592256.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            37765..39837
                     /locus_tag="PC399_001384"
     CDS             37765..39837
                     /locus_tag="PC399_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2592257.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVSTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(40050..40919)
                     /locus_tag="PC399_001385"
     CDS             complement(40050..40919)
                     /locus_tag="PC399_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2592258.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            41083..42537
                     /locus_tag="PC399_001386"
     CDS             41083..42537
                     /locus_tag="PC399_001386"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF2592259.1"
                     /translation="MKLNDIIQGLDIINIKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            42808..45087
                     /locus_tag="PC399_001387"
     CDS             42808..45087
                     /locus_tag="PC399_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2592260.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
BASE COUNT        17028 a         5436 c         7482 g        15370 t
ORIGIN      
        1 taataaaaaa atattatatt tttaaacttg aaaagtaagt aattataagc tatttaatca
       61 ctggaataaa tttacaataa aaaaataaat acaaaggctt tatcattaag gtattggcat
      121 aagttttgct tattaaacaa gtatactaat aaaattcatt taaacaggag ggagaattta
      181 tatgagtaaa ttagaatcaa caacagaaaa agttgaggta aaatggtatg ggtatgtagc
      241 attaatattg ggagcattat ttttttcagg aatatttaaa gatgcaccag gaccattaaa
      301 agtattggat tttaacaatg tattaggttc ttttggaaca ttgggaacaa ttaatgatgg
      361 tgtaggtaca ttagctgcaa actttagagg tgatggaggt actggccctc gagatggatg
      421 gctatatgca ttgacattaa taccatcagt tatgtttgca cttggagtag ttagagttat
      481 agaccactta gatggaatga aggcagctca aaagcttctt agtccactat taaagccatt
      541 attagggtta cctggatttg caggactaac tttgatagct agtttacagt ctacagatgc
      601 agctgcatct atgactaaag aacttaaaga tgatgggtat atagatgaaa aacaaaaagc
      661 tgtattttgt gcatttcaat tttcaggtgc gtctgcaatt acgaatttct ttgcttctgg
      721 agcagcacta tttccattta taggagatgt accaatattt ataccacttg ctttaatatt
      781 aattatgaag tttgttgggg cgaacttact tagattatat ttaaataaat ttgacaaggg
      841 ggaagcttaa catgagtgag gtagcaaaaa aagaagtaaa agttaaaaaa ggaaatgtga
      901 tagatgcgtt tattggagga gctagaaatg gattccaaat aagtacaaat tcaatggctc
      961 caaatgtcat cttcggtttt gctataattt ctgtgttcaa tttaacaggt cttttggatg
     1021 taatagggac aatatttaca cctgtaatga gtatctttgg acttccaggt gttgcagcaa
     1081 cagctataat gacaatattt atatcaatgg gaggagcatc tggagtaatc gcaggtttat
     1141 ttacagcagg tcaaataagt caagcacatg ttgcaattct attgccaatg ttattgcttt
     1201 caggagcgca gatacaattt atggggagac ttttaggaac agctgagtta aaaactaaat
     1261 attatccaca tatgtttgtt atagcaactt taaatggttg tattgcaatg cttataatga
     1321 attatttcgt agtataggta attataataa agatttaata taaatggaaa gtttaaattt
     1381 taaggaggag ctagattatg gaaactaaca aagactatat tgagtatgta cataaagtgt
     1441 atgattatct acacactata ccagaaaaag gatttgaaga atataagaca tcagagtttg
     1501 ttttaaaaga gcttgaaaaa atggggtttg aaaatattaa taaaggaatt gcaggaactg
     1561 gtattatagt aactatagat tctaaaaaag aaggaccagt atttgctata agagcagata
     1621 tggatgcatt ggaatttatc atagatggag aaaagaaaat gatacatgct tgtggtcatg
     1681 atggtcatac ttctatgctt ttaacagctg caaagttaat aaaagagaga aatttagtaa
     1741 aaagaggtaa gttaaaaata ctttttcaac caggtgaaga aaaattatat ggagcattaa
     1801 gagtaattga ttctggtttg ttggatgatg tagaagaaat ggtagggatg catttaagac
     1861 ctggacaaga ttgtaggttg ggaaaggcga tacctgcaat gtaccattgt gctagctgta
     1921 taatggaatt tacgattaaa ggtatcactt gtcatggttc taaaccacat ttaggagtaa
     1981 atgcagtaga tgctgtctgt ttagcagtga atgcaataaa tgcaataaga gtaaatccag
     2041 ctataccaaa ttctataaaa gtaacaaata tatcagttaa gggagaaact tataataata
     2101 taccagatga agcatttatg gctttagata taagggcaga aagtaatgat ataatagaag
     2161 aattaaaaga aaaagcaata catgctgtaa aaactgctgt aagttctata aatgcagaag
     2221 ctgtactaat ttccaaagat ggagtaccag cagcagaatt tgatgaagaa cttgtaaact
     2281 tggctgaaga aactataaaa gatgtattgg gagctgatgc atctataggt atatttaaaa
     2341 attctggtgg agaagatttc cattattata cacaaaaact aaaatgtaaa actacatata
     2401 ttggattagg agctgatgct acaccaggat ttcataatcc aaatgtaaca tttgatacga
     2461 aagctttaga atatggtgta gatatatggt gtagattggt tgaaaagaga ttgggataac
     2521 atccaaacta ataattctac atttaaaatt ataattatag aagttataga aaaagattgc
     2581 ttatgttaat tagaaaataa ttaatatgag caatcttatt ttgatataat gaaactgatt
     2641 ttggaatatt gaaaaaatat gatggaaaag taattatatt ttaaatctat tggacaaaaa
     2701 agtgatatta ttataatatt aagaattgta aaataatacc aaagtgcatc tagtaataga
     2761 cttaacaatt ataaaaattt tattcttagg agggaaaaaa tgagtaagta taattttgac
     2821 agagtaatag atagagtagg aacagattgt gtaaaatggg attttagaac taattgttca
     2881 acaaaagcac aaaaagatgg tttgcctttt tggattgctg atatggattt tgaatgtgca
     2941 gaaccaataa tagaagcatt acgtaaaaaa gttgaacata aaatatttgg atatagttca
     3001 aatgatagtg ataaatactt taatgcagtt tgtgattggt ataaaaggag atttaactgg
     3061 gaaataaata gaaaagatat tatttttagt cctggtgttg tacctgctgt tgctatttta
     3121 gtaagaatat taactaattc taatgaaggt gtaatagtcc aaaaaccagt gtattaccca
     3181 tttgaagcta agataaagag caataatagg gatgttgtaa acaatcctct gatatatgag
     3241 aatggtactt ataggatgga ttatgatgat ttagaagaaa aagctaagtg tagcaacaat
     3301 aaagtactga tactttgtag tcctcacaac cctgttggaa gagtttggag agaagatgaa
     3361 ttaaaaaagg ttgtagaaat atgtaaaaaa tatgacctat ggataatagc tgatgagata
     3421 cattctgatt taattagaaa aggatttaaa catactcctt tacagtcatt atgcccagag
     3481 tacaaggata aaatagttac atgtatagct ccaagtaaaa catttaatct ggcaggaatg
     3541 cagttgtcta atataatagt gaataatgat gaactaaaaa agaaatatca agaagaggtt
     3601 acagctgttg gagttgcaac ttcaccaaat ccctttgcta ttgtagctac aatagcagca
     3661 tataatgaaa gtgaagactg gcttaatgaa ttaaatgatt acttagataa taatatacaa
     3721 tttattgatg aatacttaaa agaaaaccta ccaaaagtaa aacttgttta tccagaagga
     3781 acatatctag catggttaga ttttactgct tatggattaa atgaagtaga acttgaagat
     3841 ttaatgttta aaaaagctaa tgtattattt gatgaaggtt atatatttgg aaaagaagga
     3901 ataggatttg aaagaataaa tgtagcttgt ccacagtctt tattaaaaga atgtatggac
     3961 agattaaaga ctatatttga aaatttataa agtataggta aaaatttata aagtgtttaa
     4021 aaataaaaaa ataataaact tgacaaattt taactaatca cttatcatac tattatgaat
     4081 aaatagaaaa cataagagag ctaaatttta ttgaattaaa ctcttaaaga ctttgaaagg
     4141 aagagtagtt atagaatgta attaaagcga gttggtgatg gtgtgagacc aatatgaagt
     4201 ctataatgaa gaacatcctg gagttgctaa ccgaaatttt aaaagagtag gcttagtcgg
     4261 aaccaaaccg ttatcatatt ggggtgtatc gagtaatatc tgtacattgt gagctgggtt
     4321 tgaatttatt tcaaactaat cagggtggta ccgcggaaaa gcaacctttc gtccctttta
     4381 tatagggatg aagggttttt ttaatggaaa aatatataaa tatagggagt gaatgatatg
     4441 agcacaataa taccaaagga ttataaaagt agtttgaacg taattgatac acaaatagct
     4501 ataaaaaaat taaaagattt ttttgaaatg agactaagta atgaattaaa tcttataaga
     4561 gtttcttctc cgttatttgt attgccagaa acaggtgcaa atgataattt aaatggagaa
     4621 aaggctgtaa gctttgatat accatttatg aataaaaatg cagaaattgt tcaatcactt
     4681 gcaaaatgga aaagattagc ccttaaaaaa tatgggtttg aagttggtag tggtttatat
     4741 acagatatga atgcaataag aaaaaatgaa gaattagaca atatacattc tctttatgta
     4801 gaccaatggg attgggaatt ggttatagat aaaagtagta gaaatgaaaa gactctaaaa
     4861 gatgttgtaa aaagattgta tggagtattt aaagatactg aaatttttgt atgtagtatg
     4921 tatgaaggta taaaagaaat attaccagaa gaaataactt ttataacatc acaagagtta
     4981 gaagatatgt atccagaatt atcaccaaag gaaagagaag ataaaattgt aaaagagaag
     5041 aaagcagtat ttataactca aataggaaaa acattaatat caggagaaaa acatgatgga
     5101 agaagccctg attatgatga ttgggaattg aatggggata tattatttta caatcctgtt
     5161 ttagattcag cattagaatt atcttcaatg ggaattagag ttgatgaaga atctcttgat
     5221 agacaattga aacttgctgg atgtgaagag agaaaagagt ttgactatca taagatgctt
     5281 ttaaatggcg aacttccgta tacaattggt ggaggaattg gtcagtcaag aatatgtatg
     5341 tatttcttac aaaaagctca tataggagaa gtacaagttg gagtatggcc acaggatatg
     5401 attcaaaatt gtagtagtgc aggaattgaa ttattataat ttaaatataa actaattgat
     5461 aaaatttata tctaatatgt tcatcaaatt aattatatcg gttataatgt agaataacct
     5521 ataattataa gaggtgataa atatggagtt tttaaaaata atcattaata tagttttaga
     5581 tattttaaaa aaaattttgg tcagatttaa aaatgctaaa tttggtttgg tttttgtatt
     5641 tgatttactt aaactacctg attttatgac agataaaaga atcaatatag ttgataaaat
     5701 aaaggtaatt tctgtattaa tttttactat atcctacttt gtatctgggg ttgatataat
     5761 tccagagatg attgcagggg catttggttt tatagatgat gctatagttt taatttggag
     5821 tataggtata gtgaatgaag aaattaataa gtatagagtt atcgcaaaaa aagataaaca
     5881 ttcaaatata attgaaaatg tagaattttc tataaaagat gaagaagaat aacaatttat
     5941 atatgttatt ctttttttta ttaacaaaaa atggttaaat tgttgacgga gaggtaaaac
     6001 tctattatca taataagtgg atatccaata tttaactaag tattcaatgt gcaagaatgc
     6061 ttaatttaaa aaagttatgg agggtaaata ttatgaagaa aaaaataggt ttagtgccca
     6121 aactaatcat cggtatcata gtcggtattt taatcggaag ttatgcacca gaaataatag
     6181 tacaaatatt agtaacagtt agtactttat tctcagcatt cctaaaattt gttataccat
     6241 ttataattat aggttttgta acagcaggta tagctgattt agcaacaggt gcaggaaagt
     6301 tattggggat aactacggga atagcatatg gttctacctt aatagcagga ttattatcat
     6361 ttgcagtatc tacattaatc ttcccaaatt ttattgatgc aagtgttgca agtcaaattg
     6421 gagacccaga ggcaggaatg ttagctccaa tattcacaat tccattagag cctatggtag
     6481 atgtaacagc tgcaatcgta tttgcatttg taatgggact tggaatatca gctttaagaa
     6541 atcatggaaa aggagagact ttatttaatt tcttccaaga atttcaagga atcgtaacga
     6601 aaacgttgtc aacagtgatt atacctttat tacctttata catagcagga acttttgcaa
     6661 atataactat agcaggggaa gtatggacaa tattaggggt attctggaaa gtatacttag
     6721 tagtaatacc tttacatttt gtatatatgg catgtcaatt cacagcagca ggtgtatttt
     6781 caggaaaaaa tccagtaaga atgttaaaaa atcaagtacc tggatattta acagctatag
     6841 gaactcaatc ttcagcagca actataccag ttaatgttgg ttgtgcagaa aaaaatggag
     6901 taagtaagca aataagagaa tttgttgtac ctctttgtgc aacaatacat ttatcaggta
     6961 gtatgatatc aataacagga tttacaactg cagttttaat gatgaatggt atgccacatg
     7021 gaataggtgt attattccct tatattgcaa tgttaggtat agcaatggtt gcagcaccag
     7081 gagcaccagg tggggctata atgagtgcac ttcctttcct accaatggtt ggaataccat
     7141 cagatggtgg gcttgcaagt ttaatgatag ctttatactt aactcaagac agttttggta
     7201 cagcatgtaa tgtatcagga gataatgcaa tagcagtagt agtagataaa ataaatgatg
     7261 gaatgagtaa aaaaggaaat aaagaagagc aaagtgctta gttttttaat aatcaaaaat
     7321 gagttatctc aaatagagat agctcatttt ttatggtata aaaaattata aaataaagtg
     7381 atttttttat ttaaaaattg ctttaagaga tgatattaat aatttatttt cattaggttt
     7441 taatatacat actctaaaga aatactcatc tagaccttca aatgaacaac aatctcttat
     7501 tattattttt tgtggaagca attgttctcg aagagattta gcagtcaatt cctttgtctt
     7561 gattttacac aatatgaaat tgcctttagt attgtatata tctaagcttt ttatattctt
     7621 aagctcttta agcaggtaat ctctttcagt gttcattaag gaaatagtat ttgatataaa
     7681 atctaaatca ctaaacatga tttcacccat tttagaagct attatgttta tattccataa
     7741 atctagattt ttatttattt catttttgac attagtgtta gatataagac catatccaag
     7801 ccttattcca ggcgttgaaa aaaattttga tgtacctctt ataacgaata aattagaata
     7861 atcatcaact aattgagtac aagagtatgt atctgtatca gtaaactcta cataagtctc
     7921 atcaatcatt aaaaatgagt ctgtattttt taaaatttct ctcacttcta ctttagtaaa
     7981 tgcaaatcca gttggattac taggattaca gattactacc aaatcataat ctttagcatt
     8041 gatagttttt ataagatttt ctaaatttat atgaaaatta tcttcttctt ttgcaaagta
     8101 tttttctata gaacaattaa ttttagataa ctctttttca tactctgaat aggctggaga
     8161 gagtaaaaga gcctgtttag ggttaattgt atgtataaat gatgaaatta gttctgtagc
     8221 tccactgcca agtactatat tatctataga acatttgcag taattggata tagaagtttt
     8281 taagtctatg taatcagggt caggatacat agataccata tctatattgt ttacaatgta
     8341 ttgtttggct aaacttgatg taccaaaagg atttatattt gagctaaaat ccataaattc
     8401 ttcctttgaa aagccatact tactagataa actatataaa ttagctccat ggtttgtgct
     8461 cataaaagta cctccaaaaa taaaattata taatcgataa aaataactga ttaatatatg
     8521 tacatataga ttataataca aaggaatcgt tattccaaat ttttaatgga aaaagcaaaa
     8581 aagtatggat aagctgttaa tttaggttta aaaatgatga aaaagatttt aattgatatt
     8641 ttggtatgct ataatatata agttaataga aggaaagcgg gaggatacaa aatgtacaaa
     8701 gttacaggaa aaatgttgac agaagaacaa ataaaagaaa aagtttatga attaggaaaa
     8761 aagatagaag aagactttaa aggcgaagat ttactagtgg taggtatact taaaggagct
     8821 agtgtatttg tttcagattt aataagatgt atagatttgg atgtaaatat agattttatg
     8881 agtgttacaa gttatggaaa ctcaacagag tcttctggaa cagttaaaat attaaaagat
     8941 ttagatgtag atattgaagg aaagaatgtg ttgattgtag aggatataat agattctggt
     9001 ttaacattaa gtaatctagt tgcagcttta aaaactagaa atccaaaatc tttaaaacta
     9061 tgtactctac ttgacaaacc tcagagaaga aaagctaata tacctgtaga ctatgttgga
     9121 tttgttatag aagacaaatt tattgttggt tatggaatag actatgctga aaagtataga
     9181 aatttacctt atataggtat agttgaggat gtagaataga tttaaatgtg aatcaaaagc
     9241 actaatttct aagggagtta gtgcttttta gttaattata taaaatttta gtaaaggaga
     9301 catctatatg gatattcagt taaatatatt attatttttc caaaatttaa gaaatccaat
     9361 tttgaacttt atatttcttg tctttacaat aagcacagaa gcaccgttat taatattaat
     9421 tacaacaatt atatactggt gtataaataa aaaatgtgga cagaaaatac tgtttgctat
     9481 aattggaaat tttgttgtaa atttaggcat caaagagttt gtaaaagctc ccagaccaat
     9541 aggaataaaa ggtcttgaat cattgagagt ttctactgct ggtggttatt cttttccaag
     9601 tgctcatact caaactgcaa caacattttg ggttagtatt atgactatat ttaaaaagag
     9661 atatcttcat ttaataggtg cagttatggt tttaggagta ggattgtcta ggctgtattt
     9721 agctgttcat tggccaatag atgtgatagc gggttggata ttaggaatat tttttacagt
     9781 tgtatttgta aaaatatttg actatattga tgataataaa aattatattt tacttgtagt
     9841 tttacttata ccatttataa tagtggctat atttctaaat agccctgaat attttgaaaa
     9901 atttggtata atagtaggat ttgtatttgg atatatggtt gaggatagat ttgttaaatt
     9961 taatacagat aataataaca aaaaaataaa ttttagtaat aaaaataagg ggagtaaaaa
    10021 tataattttt tcaagaatat gtaggttttt ggttgggata gtaacaatag gaatgttata
    10081 tattggcatt aagttgttta tttcaatgtt aatagtcact ttaaatataa gtgactctac
    10141 tatgagtata atttttatga attttattaa gaatacagta gttgtttttt atggaatagc
    10201 aggagctcca gcattattta aacttttaaa attaaattga ttttaaggta tagagtcaat
    10261 agtttaaatt aaaaaaatta agggtagaaa agagtgatta tttgaagaag gtagtaataa
    10321 taggtggtgg accagcaggg atgatagccg catcaactgc atgtgagaaa ggctatgatg
    10381 taacattaat tgagaaaaat cataaattag gaaaaaaact tgcaataaca ggaaaaggta
    10441 gatgtaatat tactaacgct tgtgaaatag aggaattaat agaaaatgta cctacaaatg
    10501 gaaaatttct ttatagtgct ttttatactt ttacaaatga tgatgtaata agtatgttta
    10561 ataatcttgg agtaaagact aaaacagaaa gaggaaaaag ggtttttcca gagagtgata
    10621 aagcatttga tattgtaaat gctcttgaaa gacaacttaa aagtaagaaa gtaaatatat
    10681 tattaaattc gaaagtagaa aaaataatat ctaaaaataa taaaattgaa aaagtaattt
    10741 taaacgataa aaaagaaatt aagtgtgatt cagttgtagt agcaactggt ggattaagtt
    10801 atccacttac tggttctact ggtgatggat ataaatttgc aatttcacag gggcacacaa
    10861 ttatagatac aaaaccatct ttaattggta tagaagtaca agaaagtttt acaaaagatt
    10921 tagaaaaatt atcattgaga aatgttgaaa taagggtgtt taatagcaaa caaaagaaag
    10981 tttatagtga ttttggagaa cttgaattta ctaaatttgg tttagatgga cctataatca
    11041 agtctgcaag ttgtagaatg aaagatacaa ggaaagaaaa ttacactata ttattagatt
    11101 taaaacctgc attagatgag gaaaaattgg ataaaagagt tcaaaaagat tttcagaagt
    11161 atacaaataa aaaatttgaa aaagcattag atgatttact tccaaaaaaa ctaataccta
    11221 taataataaa cttaagtgaa attaatccaa atacggttgt tcaccaaatt tctagagagc
    11281 agagaaaaaa cttagtccat ttattaaaaa atttaaagtt tacagtaaag agatacagac
    11341 ctattgaaga agctataata acttctggtg gtgtaaaagt aaacgaaata aattcaagta
    11401 caatggaatc aaaattagta gaaggtttat tttttgctgg agaagtaata gatatagatg
    11461 catatacagg tggattcaat ttgcaaatag ctttttctac tggatatttg gcaggtttta
    11521 attgctaaaa attacctgaa ttaaaaatgt ttaaaatcac ataatattat cagcaacaca
    11581 aaataaatta aacagaagga aggtaatcaa catggcaagt aacaataaca acaacagaac
    11641 agtagtacca ggagcaaaag aggctttaaa ccaaatgaaa ttagaaatag ctaatgaact
    11701 tggtatgagt aattatcaac aagttgataa aggaaacctt actgcaagag aaaatggtta
    11761 tgtaggtgga tacatgacta aaaaattagt tgaaatggct gaacaacaaa tggctggaaa
    11821 aagcaacaga tagttataaa taattattta taactattgg ctattagttt agctagagct
    11881 atctcaaata tagaaagtaa tttctaaatt gagatagctt ttttttatta aattattaaa
    11941 tagatatatt tttatgtaat aaattttatg tatttggata aaataataca aatgaatatg
    12001 ttacataagg agtgatgtaa aatgagtagt gtaaattttg aggatttaaa aaacaaattt
    12061 attaatagtg acttagatga aaaaataaga atatatacaa caacagaagg tttatcagta
    12121 gaacaattta aagagctact aaaatattat ccaatacaac atttatctaa actagaaaaa
    12181 gcacttggat agcagtattt aatccaaaat taaaaaggca atttatttaa tattaaaaat
    12241 attttttatt aaataaattg cctttttata ataggtttaa attattatgc ttttgatata
    12301 taattataga ttctctcatt tccaagttca atagacccag tattaataag agtaactatt
    12361 ccttctatat ctctagcagt taagtattta tatggaatta ataaattata accagtatta
    12421 cttagtaagt tttttaattg ggttatttga gagatattga aacattttgc agttttagta
    12481 gatttttttc ttaagttaga taaattttca ttttgaatat ttattaaagt ttctatatag
    12541 tttctataag atatcttaaa gtttgtctca ttgactattt tttcattaag tatactactg
    12601 ataaaatctt gagacaatat attatctgca aatttatctg ctgttaattt tccagatgtt
    12661 atgccttttt gatattttct aagaattttt ttagtatagc agtatatttt atttctcgtt
    12721 tctaaatcga gttgttctat ttgcacaaaa ccacttcctt ctaattataa aaatatttat
    12781 taaaaatcta tacttaacta ataaagcttc aataacttat ttacaataaa tactaaatca
    12841 taaaatattt gttatttttt attattacca agtataaaaa atattataat tgtaaaactt
    12901 acaaatatat gtaaaatatt taggagtgat gaaaatgtta aatgggataa taaagctttt
    12961 ttattctgaa attgtaaaaa caaagattaa taacttagat tcaagtggat attctttaag
    13021 agatgaatgt aaagaaatca gaaatataac tctaaagata gatagtgtat tttttaagct
    13081 tttaaagtta gcttttggtt caatattgct tatggtagct tttaatttaa acatggtttt
    13141 aggtatagga gtgtttttag ttggtgtagt ttatatacta tataaaagaa atatggaaga
    13201 acaagtaaaa gagcatataa ataatgtgaa aaataatatc gacatctcta aaataacttt
    13261 tatggatgaa aaagggagaa caggtctaaa tgcattaatt acattacttc ttataggatt
    13321 attgacagga tttaactgga tattagttgc tagttttgtc gttgtttttg tttttactat
    13381 aaagaatgta tgttaaaata ctaataaata gtttaatata tagggggaga aattgagtat
    13441 gactaatggc agttataaaa tgaagaattt agtggaaata tccgctatgg tgactgaatc
    13501 tacagatttt tttaatataa aagatgacat aatagaaaaa atgttagaag ttgttcaccc
    13561 tacaaaggca tgtgtgaatc tattttataa aaatgattct aaatatgcct acttagtatg
    13621 ttcgcaaacc ttagaatatg tacctcaaat ttttccaata aatagtctta aaggtgcaaa
    13681 aattgatttt gacacatatc cagagtatat acatgaggct gtaaatgaaa aaaaaataat
    13741 atatgtggaa aatgtatttg aagattctag agcagaaggt gaacgagatt tagcaaaagc
    13801 tgaaggttat attgggagaa tagtttttcc ttttataata aataacgtag ttgttggatt
    13861 tatgacttgt tttttaaaag aggatgatta tataacagaa caagatatag attttatatc
    13921 atctgttgca tcactcttaa gtttatcaat agaaataact aataaaaata ataatgtaca
    13981 aattttaatt gataaactaa gaggctcaat aagtgccata aatgaagcta ctaaaaaact
    14041 ataccttaat aagagtattg atggattttt ggaaaattta agtaaacaag cgtgtaatat
    14101 tactaatagt aaagaagctt taataattat aaatagtcat cataataagg atgctatagt
    14161 tagttgttat aatgttgagc aaaaaaagaa aacaaattta tatcctatga ttgatatgtt
    14221 tttaaagaaa gagtcattgg gggggtattt aaataattta aaaccatgct caaaaaaggg
    14281 aataaatttg gaaagttata tatattacaa acttcaggat aaaaatgaga ctataggatg
    14341 tatagtttgt gcaaatagca aaaactatac tagtgatgat ttaaatattc ttagtatatt
    14401 gtccaaacaa gtttctgttg gtatgcaatt atatgaacat aatgaaaagg aagtaaaaca
    14461 caaagttctt gaaaacgagt tgaacatatt aaataaacaa caacagctta ttatggataa
    14521 aggtaaaatg gattgtaatg atgagaaaga actttatttt taccataaac cagcaagggt
    14581 agtaggtgga gatttttatt atgctacaaa agtggatgat gaacatatag tttatattgt
    14641 tgctgatgta atgggacatg gaatggtatc aaattatgtt gttgcaatga taaaaggtgc
    14701 ttttaaagta ttatgcaatc aatacaatac accaagagaa ataatgacaa atttaaatag
    14761 gatgttatat gatgaatttg acaagatggg agtatttaca acttgtttga taggtattat
    14821 agatactaaa gaaaatatga ttactgtgtc taatgcaggt cactatagtc ctgtagttgt
    14881 aaagaaagat ggcacaatag aaaataattt aaattgtaaa aagggcatac ctattggaat
    14941 aatggaagat gctacttatg aaaataatac tctttcaatg gaagattatg caatggtatg
    15001 tatgtacact gatggaattt tagagataaa aaattcatca aaagaagagt atggtataaa
    15061 cagattagaa aactttctaa aagaaaattt tagattaaat caacagttaa tagtagaaaa
    15121 tctaaaattg gatttaaaga acttctcatc aaaagataat tatgatgatg atattttaat
    15181 agttatgtta aaggataggt agattgatta tggattcaaa cgataaaaaa ggacttaaaa
    15241 gtccagagat tataataata aatggaagtc caagaattaa taaaaattgc agttttatag
    15301 caaatgaagt aaaagctatg ctagaagagt gtggattaag ttgtaaaata tttaatatct
    15361 acaatatgga tattgaatat tgcacagcat gtggattttg tgaaaaaaca gggtattgca
    15421 agtttaatga tgatatgaag ccattatata aaatgtttga tgattcaaaa ggaactatag
    15481 taataagtcc tgtacatttt gattcaatat cagctaaatt aaagactgtt gttgatagaa
    15541 ctcaagctat atatgcaagt aaatatgtat taaataaacc ttcaattgat agaagtaaga
    15601 aacggatagg aatgtatatt gcagtgggag ggagtaaatc atatgattca cagtttaaag
    15661 gtggacagat tgttatggat tttttcttta agagtataaa tactaaactt ttacacaata
    15721 tatatttaaa caatacagat gatgtatctt atatagataa tcaagaattt aaagatataa
    15781 tatataaaaa tttaaagata tatattgatt caatataatt tgattttgta ggtaatatta
    15841 tgtataattg tattgcaata atttaaataa ttaaaaggag ggaatttcaa tgaaattacc
    15901 aatagcatta tctaataaac atgttcattt gagtcaagct gatatagacg tattatttgg
    15961 agcaggtcat gagttaacta aatttaaacc attatctcaa ccagggcaat atgcctgtga
    16021 agaaaaagtg gatttagtag gaccaaaagg aactttaaaa ggagttagag tactaggacc
    16081 agctagagct catactcaag ttgaaatatc tttagcagat ggtttcccat taggagtaaa
    16141 agcaccaata aaagaatctg gtgatatagc tggaactcca ggtgttaaat tagtaggacc
    16201 tgctggagaa gttgaaatga aagaaggagt tatagttgct tctagacata tacatatgag
    16261 caatgaagat gctgctaaat ttggtgtaac tgataagcaa atagtaaaag ttaaaacatc
    16321 tggaccaaga gcattagtat ttgaaaatgt attagtaaga gctagtgaca gctttaatct
    16381 agaaatgcat gttgatatgg aagagggaaa tgcagcaggc gttaaaaatg gagatttagt
    16441 agaattaata gttgattaat attataaata gactttatgc tagtagatat tttatttgct
    16501 agcatatttt taaatgtaat aaatttctat aggataaatt taatcaatta gattaattta
    16561 taaaataaag gggtattata aaaaatgtaa taaaaactta caaaaatatg atgtaatatg
    16621 gtaaaattat atatataaaa atttacatgt aataaggata agcattttac tttttatact
    16681 aatatgaaca aatagataaa aaagtttgaa aaatctaact taatttatta tatattgtat
    16741 acaaaattat gaaaaaaact taacatattt aattttaaaa aaaatctact taaatatgta
    16801 attaatttac aattttcaaa ggaattttat ctaatctgta gtattatatt agaagataga
    16861 gtatttgcgc gactatatgt gtgtttatag ttttgttgtg taaattatat gtaaatataa
    16921 aaaaatagac ttgttataaa caagtacaat tgtagctatc aaggaggata catattatgg
    16981 ctaagtttat gaaaacactt gatggaaata cagctgcagc tcatgttgcc tatgcgttta
    17041 cagatgtagc agctatatat ccaatcacac catcttcaac tatggctgaa gtagttgatg
    17101 agtgggcatc acaaggaaga aaaaatatat ttggacaaac agttaatgtt gttgaaatgc
    17161 agtcagaagc aggtgcatca ggaacattcc acggttcttt acaaggagga gctttaactt
    17221 ctacttttac agcatctcaa ggtttattat taatgatacc taacatgtat aaagttgcag
    17281 gagagttgtt accaggagta ttccatgtaa gtgctcgtgc tttagcatct caagctttat
    17341 caatatttgg ggatcaccaa gacgttatgg ctgctagaca aactggatgt gtattattag
    17401 cttctggttc agttcaagaa gtagctgata tagcacctgt tgcacattta gctgcaatag
    17461 aaggtagatt accatttata catttctttg atggattcag aacttcacat gaaatccaaa
    17521 aagttgaatt actagaaaat gaagattatg caagtttatt aaactttgaa gcagttcaag
    17581 catttagaga taatgcttta tctccaaatc atccagttgc tcgtggtact gctcaaaacc
    17641 cagatatata tttccaaact agagaagctt ctaataaata ctatcaaaat atagtaggta
    17701 tagttgaaaa atacatggaa aaaatgagta atttaacagg tagaaaacat agtttatttg
    17761 attactatgg agcagaagat gctaaatacg tattaatagc tatgggttca gtaacagaaa
    17821 caatagaaga aactatagat tacttaaatg ctaagggaga aaaatatggt ttagttaaag
    17881 ttcatttata tagaccattc tcaatgaaac atttcttaga tgctatgcca tctactgttg
    17941 aaagaatatg tgtacttgat agaactaaag agccaggttc aactggtgaa ccattatact
    18001 tagatgtacg tgatgtattc tatggaaaag aaaatgctcc tatgataata ggtggaagat
    18061 acggattagg ttcaaaagat actactcctt cagatataaa aactgttttt gataacttag
    18121 tttcagaaca acctaaaaat ggatttacag taggtatagt tgatgatgta actaatactt
    18181 ctttaactcc atctgagcca ataaaaatag cttctaaagg aactataaga tgtaagttct
    18241 ggggattagg ttctgacggt actgttggag ctaataaaca agctatcaaa ataatcggtg
    18301 accatactga aaaatatgct caagcttact ttgattatga ctctaaaaaa tctggtggta
    18361 taactatgtc tcacttaaga tttggtgata ctccaataag agctacttat ttaatagatg
    18421 aagctgatta tattgcctgt cataaacaat catatgttta tcaatatgat ttattaaaag
    18481 gtcttaaaaa aggtggtaca tttgtactta acactatatg ggaccaagca ggacttgaag
    18541 aacatttacc agctcatatg aaacaatata tagctaaaaa tgatataaaa ttctatacag
    18601 ttaacgctgt taaactagga caagaaatag gtcttggaaa cagaataaac atgataatgc
    18661 aatctgcatt ctttaaatta gctgaaataa taccagaaga agatgctgtt aaatacttaa
    18721 aagattctat agttaaagct tatggtaaaa aaggtgaaaa aatagttaac atgaactatg
    18781 ctgctgttga tgcaggaata aatgctttag ttaaagttga agttccagct tcttgggaaa
    18841 atgctgttga tgcagataaa ggagaagtta aagaaccaga atttataaaa aatatactaa
    18901 gaccaatgac tgcacaagaa ggaaacaact taccagtaag tacattcaat ggtatagaag
    18961 atggaacatt cccttgtggt actgcagctt atgaaaaacg tggtatagct gttgatgttc
    19021 ctgaatggat aatagataac tgtattcaat gtaaccaatg ttcttttata tgtcctcatt
    19081 catgtataag accagtatta gttactgaag aagaattagc taatgctcca gaaggatttg
    19141 aagctaaaaa agcattaggt aaaggattag aaggattaaa atatagaatg caacttagtc
    19201 cacttgactg tacaggatgt ggaaactgtg ctgacatatg tccagctaaa gaaaaagctt
    19261 tagttatgaa gccaatagat actcaacttg atacagagtt agataattgg gcatttgcag
    19321 taaatcctga tgaggtagct ccaaaaggtg atgttatgcc tgcaaacact gttaaaggaa
    19381 gtcaatttag acaaccatta atggagtttt caggagcatg tgctggatgt ggagaaactg
    19441 catacattaa agtagttaca caattatatg gagatagaat gatgatagct aatgctactg
    19501 gatgttcttc tatatgggga gcttctgcac catcaattcc ttatacttgt aaccatgaag
    19561 gtaaaggtcc atcttgggca aactctttat tcgaggataa tgctgaatat ggatttggta
    19621 tgtacacagc tgttaagcaa ataagaaata aaatagtaga tgctatgaca gagttagttt
    19681 ctatggatat ctgtgaagat gctaaagcgg tatttacaga gtggttagat tctagaaatg
    19741 atggagaagc ttctaaagta gcaagtgcta aggttgttga attattagaa aaaccagctt
    19801 gtgattgtac agatgaaaaa gctaaagagt tagtaaaagc tataaaagat agaaaagatt
    19861 atctagttaa gagatctcaa tggatactag gtggagacgg ttgggcatat gatatcggtt
    19921 acggtggatt agaccatgtt cttgcatctg gtgaagatgt aaacgtactt gtatttgata
    19981 cagaagtata ttcaaataca ggaggtcaag cttctaaagc tactccagct gctgctatgg
    20041 ctaagtttgc tgcttcaggt aaaaaatcta gaaagaaaga tttaggaatg atggctatga
    20101 catatggaaa cgtatatgta gctcaagttg ctatgggtgc tgataaaaac caatttatca
    20161 aagccgttat agaagctgaa aaacatgatg ggccatcttt aataatagct tatgctccat
    20221 gtataaacca tggattaaaa gaaggtatgg gaagaactgt agctaacgaa gcacaagctg
    20281 ttgcttgcgg atactggcat ttatacagat tcaatccaga agtaaaagct gaaggtaaaa
    20341 acccattcac tttagattca aaagaaccaa ctgcaagctt caaagatttc atacttgcac
    20401 aagtaagata ttctgctata gctaaacaat tcccagagga agctgatgga ttatttgcta
    20461 aagctgaagc tgatgctaaa gaaagatatg aaggatacaa aaaattagct gaataatatt
    20521 aaagtattat aatgcttata tagaattgat aaaagagagt gtctcaaagt gagcttttta
    20581 gttcatgagg cactcttttt gtatgaaatc aaatatattt taatcatttc aacaatgaaa
    20641 aaggccttat ctctattttg agacagcctt tttttatgaa gatattaaga tcctgttttt
    20701 acctattaaa tatcttttta agttttccat tttcatcttg ccaatacttt ttatatattg
    20761 ctctacacac taaaattaag agaacaaata atatttatag tatgtatatt tggatttgat
    20821 aattttaaaa tagatacaaa agagtatctc atttgtgttt tttatcactt agagataatc
    20881 ttttgatatg tatggaaaaa gattatttta tcctacaaag atttgtgtcc aataaattga
    20941 accaccactt ttttgtgcta ctccaacacc tattttactg aaatttggat tcattatatt
    21001 ttttctgtga ccttcagaat tcatccatgc atttacaact tctttaggag ttttttgacc
    21061 catagctata ttttcacctg ctgtcttata gcttattcca aattttttca acatgtcaaa
    21121 tggagaacca taagttggag aattgtgtga aaaatagttg ttatcaatca tatcttgaga
    21181 tttttttgta gcaacatttg atatagaaga atcaagagta agaggattta atccagcttt
    21241 agctctttct atatttacta agtcaacaac ttctttttgg taagctgaaa aactttcact
    21301 tgtagagcct gagttgttat tatcatcagg tttgttagta gagtctgaat tattgttgtc
    21361 ttcaggttta tcagtagagc ctgaattatt gttgtcttca ggtttattag actcaggttt
    21421 attattatca ggtttattag tagagcctga attattgttg tcttcaggtt tattagactc
    21481 aggtttattg ttaccaggct tattagtaga gtctgaatta ttgttgtcct caggtttatt
    21541 agtgttgttg caattcttgt cattatgatt cttatcctta caagagttat ctttatcagg
    21601 cttaagttct ggttttacag aacaatttgg ttttgcagaa aacttgtatt tagtattact
    21661 atatactaaa cttttgttac ttatatttac attattttca agtgatgaag cacttgcagt
    21721 aacagcactt gaagctgttg ctaaagttaa cactcctaaa gctaatatag tttttaattt
    21781 tgttggtata tttcttctca tcattaaaca cctcttaatc taaaaattct aaaaatgtta
    21841 ctgatttgta acctttagtg tagggggtta cccgtgaatc tatactaaca taagattaag
    21901 acttttttta ctgtaaagat atgtaaaaaa gatatatatg gaataaatag gtatatttca
    21961 gtgtgtttga caatttgtat taaaagcctg tatagtatgg aattacttag ttagagaaaa
    22021 taatattttt ataataattc cataacgtaa atttatagtt atgataaatt tttttttatt
    22081 tggttaaatt tattcagtag atgctttcgt attagcaaca aattataatt atttaaatct
    22141 ttaagaaata aaaagggtct gtctcaaaat agagataagg ccttttttat tgtttaaatg
    22201 ataggcatat gttatttcat ataaaaagag tgtctcatta agtaaaaagt tcactttgag
    22261 acaccacctt tcatattatg aattttaatt tgttttatat taatacatta gtaatcctaa
    22321 tgtaattaca ataatttctg ctattagtga accaactgca actacaatga aagtttcttt
    22381 aaatccagtt ttatgtgtta acttagataa gcttaagaat gtaagaaaaa caccactttg
    22441 tggaactata gttagtatat ttgaagctat tgtagcaaca cggtgaatta attctggatt
    22501 tagaccagta cttaaataat aatctgcaaa gtttggcatt actattccta atgcaccaga
    22561 agaggaccct gttattgctg acattgttga agtcaaaaca gtaagactta taagtggact
    22621 acctggtatt gacattataa gtttgctaaa tacatcaaaa ccaggtgcag ccattattac
    22681 tgctccaaat gcaactgcag aagatgtagc aaatatagga cctatagaac cactagcacc
    22741 tatgctcaat gtctgtatct tagatgatag aaatttatgg aataaaaaag cagataaaag
    22801 aattgcaact attaaagcta tgtaaattac attttttttc ataaattctg aaccaaaagt
    22861 acttccagtt atagctataa ttacgagtgc taatagagga aaaatacttg caaaaaatcc
    22921 tggtaattct ctatctaaag attcttcttc taattctgaa gtataagttg cataagtttc
    22981 accttttcta atacttttgt ttagagcata tttcatatag acaagtccaa acgtagcaca
    23041 acctatacta cctacaatac ttggtatagt agctgcagta agagacgtat ttagatattg
    23101 aataggtata acattttgaa tggctggtgt tccaggaatt atagtcatag taattgttgc
    23161 aataccaagc caaagtggta tctgaataag gttccaagaa atatcaagtt ttttaaatag
    23221 agttcttgca agtggaatga ccgcaaacat aactacaaat aagcttatac caccatacgt
    23281 taatataaaa cttactataa atatagcaac taaaactcta taaggatgat tagagccaaa
    23341 tttatttaaa ataaaatcag ctattgatac agtagctcca ctttcttcca taaatttagc
    23401 aagtattgag cctaataaga atattgcaaa ataactcatt atatagtttc caagtgcacc
    23461 catataattg tttttgtcag cacctagcat tgatgttaca acatccattt tatttaaaat
    23521 aacaactatt aaagttgcta ttggagcagc tatagttata tgaatttctt taactgaaaa
    23581 atatataata gctatgatac caataattat accaattggt gcgataatac tcataaatat
    23641 ccctccttag tagaaggacc tgaagaatac tttcttcaag ccctaaatat aataactatt
    23701 gtgcagtata tcctccatct agtaagcaag cttgtcctgt aataccctta gcattatcac
    23761 ttgctaagaa catagcatag tcggcaattt cttgaacatc taataggcgt ttttgtggta
    23821 caagtgggta taaaacttcc tctaagacat tttccaatgg tatattacga gtttttgaaa
    23881 ggtcattgaa ctgacctctg acaagtggag tatctacata tccagggcat aaagcattaa
    23941 ctgttatacc agaatcggca gcttcaagag cagcaacttt tgttagtcca ataagtccat
    24001 gttttgctga gttataagca gcctttccag caaatcctat tacaccattg attgaggcca
    24061 tatttattat acgaccaaag ccttgttttt tcattatagg aaatgctttt ttagtagcta
    24121 taaaaggtgc tgtcagcatt atttttatca taaattcaaa tttatcagtt gggaaatctt
    24181 ctatcataga aacatgttga agtccagcat tgtttattaa tatatctaat ctgccatact
    24241 tttcaacagt tttgtctata gcgtggttaa tttcttcttc cttagttaca tcacacttta
    24301 cactcatgca atcataacct tttttttgta actctgttat ggtattattt aaagcttctt
    24361 gatttatatc agagaacatt actttagcac cattttttaa gaacgattca gctatttgtt
    24421 tacctattcc acttgcagct cctgttacga atacaacttt atctttaacc attttcattt
    24481 cctccaattt tattttattt ttatattaat attttaagca atacaaattt atatgtaata
    24541 cgtctaagag atttttattt tctagtttta attattttaa acaacagata tttttccatt
    24601 taatgaatct gacaatatta aaggagcctc agtagcagaa ataacatcat ctaatgtgaa
    24661 agctggattt atttctttaa gtaaaattcc atctgatgtt acttccataa ctcccatttc
    24721 agtaactatc aaatctacag catttgttgc agtaagtgga agagtacaat tatttaatat
    24781 ttttgcagaa ccttttgaag tatgttccat tgctacaata acttttttag ctccagttac
    24841 taggtccata gctccaccca tacctggaac catcttacct ggaatcatat agtttgctat
    24901 attaccatat ttgtcaacct gaagtgcgcc aagaacagtt atatcaacat gacctcctct
    24961 gattattcca aaagaagtag cactatcaaa gaaacatcct ccaggaagta tagtaactgg
    25021 ttgaccacct gcatttacaa tagtttcatc acttgaattt ccatcttcgg ctgaacctaa
    25081 acctaaaaag ccattttcag attgtaagat aacgtttaca ttatccggaa tataatttgc
    25141 tactttggtt ggtagaccta ttccaaggtt tacaacggct ccgtctttta attctttaga
    25201 tactctattt gcaatatatt cttgcatttc taatttatcc attataaatt acctccttca
    25261 atgatgtaat tcacaaaaat acctggtgta ttaacttcat ttgggtcaat atcaccaatt
    25321 tcaacgattt catctgcttc aactattgta attttggcag cagatgccat taaattatta
    25381 aaattgttca tagagccttt ataaactaga ttacctgctt tatcgacctt tgatgcaaat
    25441 agaagagcaa catctgcata aattggtttt tcaagaaggt attctttgct atcgacaaca
    25501 attttttgct ttccctcctc aacaagagta ccaataccag tcggtgtgag tatacctcct
    25561 agaccataac cagctgcacg tatttgttca gcaagagttc cctgaggaac taactcaaca
    25621 tctgtttcat tgttattcat ttgtcttcca gtttctttat ttaatcctac atgagaagca
    25681 attatctttt taaattgctt atttacaacc attttgccaa ctcctttgtc aataaagcca
    25741 gtatcattgc aaattagtgt caaatctttt atatttttac tacataaata atcaattaaa
    25801 ttttctggtg aaccattggc catgaatcca ccaaccatta ttgtatctcc atcttttaca
    25861 tgagatagag cttcatcaat gctaactatt ttattcatat ttcctcctat cgtgaaacca
    25921 ccattgatat tccttgacca ccaccaatac aaagtgttgc aagtccagtt tcaactttac
    25981 gtttttgcat ttcataaatt aaggtaacaa gtattctagc tccacttgct cctattggat
    26041 gtcctaatgc tattgcacca ccattaacat ttaactttga tgaatctatt tgaagttcat
    26101 ttttaacagc taatgcttgg gcagcaaacg cttcatttgc ttctattaag tctatatcat
    26161 tgatgctaag tccagctttt ttcagtgctt ttctagttgc agggattgga ccagtaccca
    26221 tgacttcagg ttcaacacca gctgaagcat aggattttat tttagcaagt ggtctaatac
    26281 ctagttcgtc tgctttttgt tggctcatta gtattaacat agccgcacca tcattaatac
    26341 ctgaggcatt tccagctgtt acagtaccat cttttttgaa tgcaggttta agtttagcta
    26401 aaccttcaaa tgtcatacct aatttaggat attcatcttt atcaatagtt tctatttttc
    26461 ctcgacgtat taaaacatct actggaacga tttcttcttt aaagcggttg ttttttatag
    26521 cattctcagc cttactttga ctttctaatg ccaatttatc ttgcatttct ctagtaaatt
    26581 caaatttagt tgcaatattt tctgcagtta ttcccatatg atactgattg aaagcatcaa
    26641 taaggccatc tgttagcata ctatctacca tggttatgtc acccatttta ctaccaaagc
    26701 gagcagttgg aacgatataa ggagcttggc tcatattttc agtaccacct gcaattacca
    26761 catcattttc accagatgtg attgattggg cagctagctg gacactttta agccctgaac
    26821 cacataattt atttactgtg tagcttggaa cagaatttgg tatacctgca tttatagcaa
    26881 tttgtctagc aacattctgt ccaagccctg tttgaagaac atttccaatt attacttcat
    26941 ctatttcaga taaattaagt cctacacgtg atatcgcttc tttaacagtg atagttccaa
    27001 gttgaacagc tgaagtattt ttaaatacac ctccaaaact tcctataggt gttctgactg
    27061 ctgatacgat tactacatct ttcatatcta atagttacct ttcttgaatg ataattaaaa
    27121 attttctgaa taaagaaatt aggaaacact ataataaagt acttttttaa tacaagcttg
    27181 tttttaagta tttcaattac ttttattcct aatgctttag gttttcctgt ttctaattat
    27241 agattacggc gaaaaaagcg ttttgtctaa gtctaatttt ttatatagtg tataagaaaa
    27301 atttatagga taaaaattta tagtagattt agaaaaatat tttgttaaaa tattaatgaa
    27361 attatgttga ttttcctata aaatatcatt agaagaaaaa atataatcta aattatactt
    27421 ttggggaagg gggtttaaaa tggatataaa acagttgaaa tattttgttg aaatagtgaa
    27481 atctggtttt aatttatcaa tagcatctaa gacacttcac atatctcaac cagcattaag
    27541 tcaaattata aaaaattttg aagaaagtga agatgtatat ttatttgaaa ggtataaagg
    27601 caggttaaat gggttaacac cagcaggaga aagattttat gtaaatgcag aacatatcat
    27661 aaatgaatac aaaaatatga tggaagattt aagagaagat tcagttcaat ttaaaggaaa
    27721 agttagaata ggaatacctc cacttatact tggaatagca ttttcagatg tagttgcaca
    27781 gttagttgca aataatccag atatagaatt tgatatagta gagaagggtg catatgactt
    27841 aaatagaatg cttatcttac aggaattaga ttatgcagta ttgttacatc cacataaaat
    27901 tgataaaagt gtagtaactg aacatgtgct tcaagaagat gaacttacag catttttaaa
    27961 tataaatcat ccacttgcta agaaggataa gatagattgg aacgatttga atgagcagtt
    28021 acttgcaata tttaatccta cttttatgat acatcataag ctaatgcaaa aatttactga
    28081 tgaaaatgtt aagttaaaaa gatatataat gtctggttca tgggattttt tgttgctatc
    28141 aaccacaaat tctgatttta taacaattct accatcacca gttcatgatt tttttgaaaa
    28201 tagcaaaatt atagaaaagc cttttaatca tcctattaca tggaaagtta tattatgtag
    28261 acctaaaaaa gaacgttata gccatgtaga ccaacatgta tttaaattta ttatagattt
    28321 ctttagtaaa aagaactctt aaaaatgtta ttaaaataac aaataaataa atcaattttt
    28381 attataataa actattagtt tttcatctat atgtttgaga gcaataatag gttttacata
    28441 acatggtaga gtagtattta aagttataat atacaaaata tgtatttttt taaacgaaaa
    28501 ataaaaacag tgtatatctt tagataatca ctgtttttat tttctatata aaatttgtta
    28561 agtattacta gaaaattttt tgcttattat agtcaaactc atgactgtta aaaatataag
    28621 taatcctgga aatacagcta aatataactt gtcaaaaata tgtctttgag catcttgaag
    28681 catattaccc catgatgcaa gaggcaattg gattccaaga cctaaaaaac taagagcaga
    28741 ttcagttaag atagcattag caatatttaa cgaaccagca actacaatag tctcttttaa
    28801 gtttaaaact atatgtctta atagtatatt tctagtcttg ataccagtag ctttagcagc
    28861 tagacagtat tcattttcct ttatttttaa tgtttcgcca cgtacaattc ttgaaacttc
    28921 catccaactt aagaatccaa taattagtat aatagtggaa actttaggtg ttagataagt
    28981 atttaataca atcaaaacta agaaacttgg tatactcata agcatatcta ttaatctcat
    29041 caagaattgg tctagcttac ctccaatata tccactaata acacctacag cagtaccaaa
    29101 tactacagaa ataagcattg ataaaatacc aacactaagt gaaatccttc caccataaag
    29161 agctctagag aagtaatctc taccaagttc gtctgtacca aagatatgct ttaagctagg
    29221 ttcttgaagt ttagaagtag tatcaattgc attagagtct atatttaata taaaagaagc
    29281 tataattaat atgattatta tgctaagagt aatacatgca aaagcaaagg atttatcttt
    29341 ttttaatttt tttaagatac ttgtaaacac aactagaatc ctcctttgct agattttatt
    29401 ttagggtcaa ttaataagta tagtatatca gcaactaaat tacctaaaat taaaaatagt
    29461 gctgataaca tagttattgc cataattaca gtataatctc ttgaaaaaat tgaatcaatc
    29521 attaatcttc ccatacctgg ccaagagaaa actacttctg taacaaatgc accagtgact
    29581 aatttttgta tactcatgcc taggattgta accaatgatg tcagtgaatt tttcaagaca
    29641 tgtttaaata gtatgacttt ttctgataat ccttttgctc ttgctgtaat aacatattgt
    29701 ttttttaatt gttcatttac actagcttct acataatttg taaaaatagc agtattatat
    29761 agacctaaag ttattacagg caaaactaaa tgttttacta agtctccaat actatcattt
    29821 ccaatagtat gcatacctcc tgatgggaaa atgttaagtt taactgaaaa aattgaaatg
    29881 agtatcatac ctatccaaaa agaaggtata gaaacaccta tataattaaa aacagatgat
    29941 actttactag ttattgaatt tttatttaca gctaaaaata ttccaagagg tatagctacc
    30001 aatattgaaa atatcaaaga agtacccata agaataaatg tatttcccat tttttccata
    30061 atttgttctg taacgggcat atggtttata taagaaatac ctaagtttcc acttaaagtg
    30121 tttttcaacc agtttaaata tcttatatat actggtttat caagccccat attaaccctt
    30181 atattttcaa tatcctgtga agacattaaa ggatttatat atgcatctgc tgggtcacca
    30241 ggagctaatt ctaaaagtcc aaatgataat atagatatta agattaacat aggtattaaa
    30301 acttttactc tattcaatat taattttaac ataagtaaat tctcctaatt aaaagagatg
    30361 tctcgtttta aaataataaa acaatattat ttttagacat ctccttttct aattaataat
    30421 ataaaatttt acatcaatta ttctacatac agtttagacc aatcttcaaa taatattgca
    30481 ggagatggtt tagcatcttt tattcctttt agattttttt gagctgccat taaattttgt
    30541 tcataatcta tagtgtaaat aggcgcatcc ttagatatat cctgttgtat ttgcttatat
    30601 atttcttcac gtttttcctt gtttaactca gtaaatcctt tttcccataa agcatctatt
    30661 tcagcattag aatatttagt ttggttgcaa tattgaccag tttcaaacat acctctatat
    30721 gggtttggtt ctaatcccca agcataacca tttacataca tatcacattc agtgctatta
    30781 ctaaatagta tattgaagaa tgccttactt tcataaggaa ctatttcagc ttctatacca
    30841 atttttttca actcttgttg agctactaag gcatagtttt tatgcccaaa tctacctgtg
    30901 ttataaccta tttttaattt atcaatttta acaccacttt tatccaataa gtctttagcc
    30961 ttatttgtat cttggtcata tccttcaaca ccttcttctg tatagaagtc tgcttctgga
    31021 actaatatag atttagcagg tacagaacct tcttttccat atgctgattc tatcatttca
    31081 tttctattta aggcatatga taaggcttgt cttacttctt tgttagccat gtttgatata
    31141 ttttggttaa atacaatgta gtttaatctt tcttcactat aagtataagt ttgtaagttt
    31201 gagtttgatt ttgccttttc atatccttca gcagatgtct tcataagtga aatttcgcca
    31261 ttatttaaag ctgcttcttg agatgcttca ttaggaatta tttttagcgc tatagaatct
    31321 gccttaggtt ctccaccaaa ataatctgca ttcttttcaa atactatgct ttcacctttt
    31381 ttccattcct taaacttgaa tgcaccagaa ccaactggat tattattttt ttctgactta
    31441 gctatatttg attctccttc aaacacatgt tttggtattg gagaaatttt agatatacca
    31501 tataagaaag attcacttgc agttggtaaa gtaatcttaa ctgttaaatc atcaattttt
    31561 tctactttaa ctggtttctc acccaccaat aagtattgtc ttgatggtga attttgtttt
    31621 tcgtctaaaa ctgtattaaa tgtaaataag acatcttcag cagttattgg ctttccatca
    31681 tgccatttta aattggattt taattttata gttaattcct tactatcttt aaaatctact
    31741 ttttctgcta aataatttac taaatttcct ttttcataag tataaagagg agcataaact
    31801 atatttgatg caagagagtc ttctttaacg tttgcaaaag ctccattgat tatatctggg
    31861 tcacttgcca taggtattac aacagttcca ccagatttgc ttccactaga cttgtcttta
    31921 tcattggaag cacaggctgt aaatgtaagc attaatgatg aaactaagat taatgatgcc
    31981 aattttttaa atttcatatt cttataaaac ctccataaaa taatatttaa cacatatttt
    32041 ttattgtata atagtaatca ttattaaatc aatatatata gaaattaact attatattat
    32101 tataattaat agaaaaaaat aataaaatgt aagaaaatgt tgtaatttaa atttttataa
    32161 aatacaatca aaaggtgtat gttatcttaa gtaattttgc tgagaggaga aaataatgag
    32221 taaagcacta attgaggtta aagatttaaa agtttatttt catacagata aagggattgt
    32281 aaagagtgta aatgaagtaa gttttaatat aaatgaaggt gaaactattg gaatagttgg
    32341 agaatcagga tgtggtaagt ctgtaacagc catgtctttg atgaagttac ttccaacatc
    32401 aaaaatagaa ggtggagaaa taatctttcg aggcaaagac attttaaaga tgaatgaaga
    32461 tgaacttatg ggaataagag ggaatgagat atcaatgatt tttcaagaac caatgacatc
    32521 tttaaaccca gcgtttactg ttggaagtca aatcattgaa ggtattatga tacatcaaga
    32581 tttatcaaag gaagaagcta agaaaaaggt aatcgatatg ataaaactgg tagaaatacc
    32641 aagagctgaa gaaatatata attcatatcc acatgaatta tcaggtggta tgagacaacg
    32701 aataatgata gctatggctt tatcatgtaa tccaaaatta cttatagctg atgaaccaac
    32761 aacagcttta gatgttacta ttcaagctca gattttagat ataatgaaaa atataaaaga
    32821 aaaattaaac acatctataa tgatgattac acatgatttg ggagtagttg cagaaatgtg
    32881 tgacaaagta ttagtgatgt atgctggcaa aattatagaa gtggcagagg ttgttgaatt
    32941 atttaaaaat ccaaaacatc cttatactat tggacttcta aaatcaaagc ctgctcttgg
    33001 aaaaaacaaa gataaaagat tgtattcgat tccaggacaa gtaccaaatc caattggaat
    33061 gccagattca tgttatttta gtgatagatg tgaaaaagta tgtgataaat gtagaactca
    33121 gatacctcct ttaatagaat tgaatagtgg tcatagtatt gcatgttggt tatataaaaa
    33181 ggaggagata taattgaaaa aagaagagag tttattaaaa gtagaaggtc ttaaaaagta
    33241 ctttgaagtt aaatctggat tatttgataa gaaaattaaa tctgtaaagg cagtagatgg
    33301 aatttctttt gatttaaaaa aaggagaaac tttggctata gttggagaat cgggatgtgg
    33361 aaaatcaact gcaggaagaa ccattcttag attaattgaa aaaactgaag gtaaagttga
    33421 atttaatggt gtagatatat ataagttaga gaaggaagaa cttagaaagt taagacccaa
    33481 aatgcaaatg atatttcaag acccatatag ttcattaaat ccaagattaa atgtggaaca
    33541 aataatatcc gaggcagtta tagaacataa tttagtgcca aagaatgaaa taaaagataa
    33601 tatattaaat gttgtagaaa gttgtggtct ttctaagtac catttaaata gaaaaccagg
    33661 tgagttttct ggaggtcaaa gacagagaat aggtatagca agagcattag cattaaatcc
    33721 agaatttata gtttgtgatg aacctgtttc tgcactagat gtttctattc aatctcaaat
    33781 tataaattta cttatggatt tacaggataa atataaactt tcatatttat ttatatctca
    33841 tgatttgagt gtagtaaatc atattgcaca tagagtttgt gttatgtatc taggttctat
    33901 aatagaaatt gcaactaaag gagaacttta cttacattct caacatccat atacaaaagc
    33961 acttatgagc gcagtaccaa tacaagaccc tacaattaag aaaaatagaa ttatattaaa
    34021 aggagacata ccatctccaa caaatccacc aagtggatgt aagtttcata caagatgccc
    34081 ttattgtaag gaaatatgca aaaaagagat tccagtttta aaagaaatat caagaaatca
    34141 ttttgttgca tgtcatctag tatagatatt tcaaaaatta aatttgatgt atataataag
    34201 aataatagaa taaactgaaa atttttttga ttaagatggg atttcatgct gttaatatta
    34261 agttagttga tatataagtt taatctataa tattattatt gaaaagagag gtagttatgt
    34321 caattttagt aacattgttg ggattattgg tttgtacaac gatttcaact gtatttagca
    34381 aaaagtggag taatattcct cttgcaatat atcaaattgt actagggatt atattgtcta
    34441 ttttgccatt taaattctca ttttcattta atccagaaat atttgtaata tgtatcatag
    34501 ctccattact attcagtgaa ggacaaaatg tatcgagaaa agagctatta gagcttagaa
    34561 aaccaatttt gcttcttgca tttggtttag ttttaataac cgtgtttgca ggtggaatct
    34621 ttatacattt tcttattcct gggatgccat tatctgtttc atttgctttg gcagcagtaa
    34681 tatctccaac tgatttagta gcagtaaaat caattacaca aggattaaat tttcctaaaa
    34741 atataatgag tatacttgaa ggtgaatctt tattaaatga tgctgctgga gtagtagcat
    34801 ttaaggttgc agtacttgct acagttacag gagttttttc aatagaagag gcagggattc
    34861 aatttatgat tacagctttt ggaggaataa tagtaggaag tattttagga tacataatta
    34921 taaaaataag acttagttta cacaagtgga atttggaaga aattccaatg gtaatagtta
    34981 tacaaataat gactccactt tttgtatatt ttgtagcaga agaaatagga gtatctggaa
    35041 tcctagctgt tgtaatggca gggatagctc atggaattga aaaagagcat ttacaaaata
    35101 caactactaa attaaggata atatcagaca atacttggta tgttttggag tatgtattga
    35161 atggatttgt ctttacactg cttggttttt tgttaccaag tatatataca ggacttagta
    35221 gtaaaaatga aaatatggca ttggaattga cttttatttc tactttaatt gtacttattt
    35281 tatttgttat aagatttata tgggtatatt tatggcataa ttcttttata aaacctaaaa
    35341 agaatccttt aaataatttt tttgtaggtt ttttaggttt taaggatgaa gaagttataa
    35401 aagagagtat ttctaaatgt aaatactcac taatagttgc tacttgtggt gttcatggta
    35461 catttacact agcaactgcc ttatctatac ctttttattt ggctgataaa gatgtatttc
    35521 caatgagaga tactgtttta tttatatcat cagaagttat tcttataagt ttagtattgg
    35581 caacaatttt actgccaaga ttattaaaaa ataacgtaca agagtcagaa cctttattat
    35641 ctgataaaga ggcatataaa ctaattttga aagaagccat tttaaaactt agtaatgaaa
    35701 aagcacaaga agtacaacca gttatacatg atttaaatga acagttggta gactcagaaa
    35761 aaggaatact aaatactcca gataataaac ttataagtat gatgattgca gaagcagacc
    35821 aacagggatt attagctgtt ttgaaactat tagaagatgg taaaatttct gatagagcct
    35881 ttaaattgta tagattttat attacaaaat caagaaaatc tgttcaaaaa tctatattta
    35941 aaattgtaaa attaaaaata agcatgtgga ttataaatag aaaagtaaag aaagaagtaa
    36001 aagaaaaaat tacaaaaata gtatctgaga ataaagaaat aatcaaagag tttcaccatg
    36061 catatgcact ttctactaaa actgctatat catttataaa taaaaatact gatgaagata
    36121 acagacatga ggccttaata gcaatcagat tctataatcg ttatttgaat tggattacta
    36181 gaaagattca aaatgatgat aagtttgagg caagagtaga atactacaga gtaatggcga
    36241 tacaaagtca aagagacagt gttcaacaat tagttgaaac taagcaaata tcaagagaag
    36301 tggctaaaga gttattggaa aatattctct atgatgaaat gatgatgata gaatcataaa
    36361 tataatggaa taaaatttga tttttgatgt taaaattaat actaaaaaat aggaaatagt
    36421 tttcaataat ttacttgaca tatatattta tttataatag aataaaacca taaattgaat
    36481 acaatatata agcattaaca agctggattg ttactggtaa gcaggcaata cctaggttaa
    36541 ggtatacagg tgaattgtat cttaatttag gtattttttt atgaataagg gaaattgtat
    36601 tttgggagag atattgatta tgattattca acaaatttat aataataatg ttgtattagt
    36661 tttagatgaa aatataaaaa aagaattaat attaacagga tgtggaattg ggtttcaaaa
    36721 aaagaaaggt caagaaatag ataaaagtaa aattgaaaga acttttgtaa tacaagatga
    36781 aagcttttta gataaaatta gtaaactagc aagccaggtt gatgagaaat tttttgaggt
    36841 tagtactgaa ataattgctt atgcagagga gaacttgaat actaagcttt atgaatatat
    36901 ttatgtagca cttactgacc atattgcatt tgcaattaaa agatatcatg aaaacataac
    36961 tataaagaat gatttactac atgagataaa aagaatacat aaaaaagaat atgagattgg
    37021 gaaatgggct gtagattata taaataaaga atttgatgtg aaatttccag tagatgaagc
    37081 tggttttata gcaatgcata tagtaaattc aaactataag ggaagttcaa aggaatcact
    37141 attaattact aaaattgtaa aagacatact aaatattata agatattatt atagagttga
    37201 atttaaagaa gatgatataa attatgatag gcttctgact catttaaaat tctttgcaaa
    37261 acgattagta aaaaaagaaa aaatcaatga tacaaataat gaaattatag atataattaa
    37321 agtaaagtat gaaaaggatt atgattgtgc atataaaata aaaactcatg tagaaaagaa
    37381 ttatgattac tatgtaagtc aagatgaact actttattta acattacata tcaaaagagt
    37441 tatatctgta ctgaatttat aataaagagt agtttaatac agaatgtata gatttaatat
    37501 attatgttta attttaatat attatttaaa atttaatata aaataataat tttaaaacat
    37561 taagattgtt actgataatg caggcaagac ttatgtttat aaaaaaatta taatagttta
    37621 tttttaattc tgaaattagg ttaatatagg tttatttata ttaacataag tttaatttta
    37681 gaattaagta tactaatgta actttttata aacttaagtc ttttttattt aaaaaaatat
    37741 aaaataataa ggagagaaaa gtaaatgaaa aaagtatttg gtgttttaca aaaagtagga
    37801 aaatctctaa tgctaccagt tgcattactt cctgcagcag gtatattact tggcgtgagt
    37861 aatgctttag caggagacgc attgttggct cattttccag ctttggcgaa tcctacattt
    37921 caacttataa taagtataat ggaaagttca ggtcaaatta tatttaataa cttaccactt
    37981 atatttgcag ttggggtagc tgttggttta actgaaggag aaggtgttgc agcattggca
    38041 gccatagtag gttttttaat tctcaataca tctatgggtg ttatggcagg tgttacagaa
    38101 gatatggtag acaattctat gtatgcaaat attgttggaa taccaacaat tcaaacaggg
    38161 gtatttggtg gcattattat agggatagtg gctgcttatc tttataaaaa gttttataaa
    38221 attgaattac ctcagtatct aggttttttt gcaggtaaaa gatttgtacc aatcgttact
    38281 tcattagttg ctatagcaat aggaattata ttgtctttta tttggcctcc aatacaagag
    38341 ggattactta gtttctcaaa aaatgttata gaagcaaatc aaacactagc agcatttatg
    38401 gaaggttctt tggaaagact attaatacca tttggtttgc atcatgtttt ccataatcca
    38461 ttttggtatc aatttggaga atatgtaaat aaagctggtg agcttgtaat gggtgaccaa
    38521 aagatattct ttgctcaact taaagatgga gtaaatttca cagcaggtac ttttatgaca
    38581 ggtgcatttc catttatgat gtttggtctt ccagcagcag ctcttgctat ggtgcatgaa
    38641 gcaaagccag aaaagaaaaa atttgtagca ggtataatgg catcagcagc attaacctct
    38701 tttttaacag gaataaccga gccaatagag tttgcgtttt tatttgttgc accagtatta
    38761 tttgcaatac attgtttatt ttcaggttta tcatacgcaa ttatgcaatt attaggagtt
    38821 aaaattggat tcacattttc aggaggatta atagatttct tattatttgg tgttcttcct
    38881 aatagaactc catggtactt ggctatcttg gtaggtattg gttttgcagt attatactat
    38941 agtgtattta gatttatgat aagaaaattt aatttaaaga caccaggaag agaagatgag
    39001 tcagaaacta acgctgaagt cattgaaatg tctaagaatg aattagcatc agagattttg
    39061 attgctttag gaaataaaga caatttaaag agcctagatg cgtgtataac aagacttaga
    39121 ttggttgtaa atgatataaa taaagtagat aaagaaaaat tgaaatcact tggagcagca
    39181 ggtgttatgg ttattggcaa caatgttcaa gctatatttg gaccaaaatc ggattcaata
    39241 aagtctgata taaaagatat aatagaggga aaaacaaagg ttgaaagtac tgttactaaa
    39301 aatgttgata aaaataaagt gaaaaaaaat atggaagttg caaatatgga aaatgcttta
    39361 gtagaaaaga ttgattcatt aacaaatgga gagatattag atatagaaga agtgccagat
    39421 tctgtatttt caagtaaact gatgggggat ggatttgcaa ttaaatctag tgatggattg
    39481 atttattcac cagttgatgg aactattgga gttatatttc caactaagca tgcaataata
    39541 ataaaatccc aaaaaagtgg agtagaaata cttatacatt tagggataga aactgtaaat
    39601 ttagaaggaa atggatttga agtatttgtg aatgtagggg atgaagtaaa agcgggagaa
    39661 aaattagtta aaatggactt agaatatatt gaaaaaaatg gtctatcaac aataagtcct
    39721 gtagtattta cgaatttaga acaaaatcag aaattaagca ttaaaaaagg agtaagcaca
    39781 gcaaaagagg agaatagagt aagtatatta agagatgata ttgaaaaaat aggatagcta
    39841 tatgatagtg taactaaaag ttatgtggga tttaataaat atataaatag gttcagttaa
    39901 catgcaattt atttaatata tattttaagt aatagaaata tatatcatta tattttgata
    39961 tattttaaaa cattaaaaaa cttgaggtat tagtaagata aatacactaa tacttcaagt
    40021 tttattaaaa taaaatttac ataaattatc tattcatcta aaagagtaat atccaaagga
    40081 tttttattgt ttatataatt tttaggagat accccgtagt tttttttaaa tgcccttaca
    40141 aaactagaat aatcgttgaa tccacattta ttacaaactt cactcataag gtgaccattg
    40201 gttattagtg actttgccaa aattaatctt ttttgaacta tataattgtg tatagacgtt
    40261 ccagtatgag atttaaattt tctcataaga taatacttac ttataaaaaa ttcagacgat
    40321 atgctatcta ttgaaagctt atcatctata tttgaattta tatagtttaa tactttctta
    40381 atagtttcat cataaaaaac atcttctgtt gattccatat tttcattttt taggaaaatt
    40441 ctgtttaaga atatcataag ttgtataaat aatgcatttc tcaatatttc atttccaaat
    40501 tcagtattgg attcggaaat atttatttgc tcaataaaac tcttaataga ttcaatatca
    40561 tgtatattta aacgcaatct attgcattta ttctctgtag ctattttaaa acatttttct
    40621 aagtcactac tagcatcact attttttttc ataaattctg ggctaatcca tattgcaatt
    40681 ctctcataaa agacatttga atctataact ggtttatgta tttcattatt gctgataaag
    40741 agtatatccc aaggctttag tttataggga ataccatcaa caaaataagt tacatttcca
    40801 tttataaata taattatctt attaaaatca tgattatggt attcaaattg tatatctttt
    40861 ttatctctaa tattaaatat tttaaagtta tcgtttaagt acccgatttt attatccata
    40921 gtttttatag aagacctttc ctcagttata ttatagaaaa ttatagagca ttttttgcaa
    40981 tgtttcaagc atttttaacc attcacatag taaaaaattc ttaatataat attatatgta
    41041 gtgtaatttt agcagaaaga gtttaaaggg aggaaagcat ttatgaaatt aaatgatatt
    41101 atacaggggt tggatataat caatatcaag ggtgaattaa atatagatat taataatgta
    41161 caatatgatt caagaaaggt tacaaaaggt acattattta tatgtataaa aggatttgtg
    41221 tcagatggtc ataagtatat aaaagatgcc atagaaaagg gagcaagtgc atttcttgtt
    41281 gaagaagatg tagaaataaa aggatgtaca tttataaagg taaaagatac aagaaaagat
    41341 atggcaaaag tagctgataa tttttacaat catccatctc aaaaatttaa cgttatagga
    41401 gttacaggta ctaatggaaa aactagtata actactatac taaatgagat attaacttta
    41461 aataagaata aagttggact tataggaact ataaaaattt ttgatggaga aaaagacatt
    41521 gtttccaatt caacaactcc agaaagtata gatttacaat atcattttaa taatatgttg
    41581 gataatggat gtgactactg tgcgatggaa gtttcatcac attcattggc attaaataga
    41641 gttgatgaaa cagactttaa actaggcata tttacaaatt taacaccaga ccatttagat
    41701 tttcataaag atttagagga ttatagaaaa gcaaaagaaa aattattttt taagactact
    41761 atggcaaata taataaacat agatgatgaa ggtggaaaaa agatatatga aaatataaaa
    41821 ggtataaatg taccttgtta cacttatggt gttgatacta aggctgattt tatggctaga
    41881 gatataaaat cagattcgga tggagtatct tatagattaa taacaccttc atatgaagaa
    41941 gtaatattta taccagttcc aggaatgttt acagtatata atactttagc agttatagct
    42001 gcttgctatg tacttggcat acctaaacca atatataaag aaggccttag actttctaat
    42061 ggagtatcag gaaggtttga aacagttcca aatgataaag gtataagcgt aattgtagac
    42121 tatgctcata ctcctgacgc acttgaaaat gtactaaaga caactcaaca atttgcagaa
    42181 ggaaaaataa tttctgtgtt tgggtgtggt ggagatagag atactgaaaa gagacctttg
    42241 atgggagcaa ttggacaaaa atattctgat ttatgtatta taacttctga taatccaaga
    42301 actgaagaac cagaggcaat aataaaagat atacttgaag gaattgacaa gaaaaaagaa
    42361 aattaccatg ttgttgttga tagggaacaa gctatagcag aagctataag tatggcaaaa
    42421 aaggatgatg ttgtaattat aacaggtaag ggacatgaaa cttatcaaat tataggtaaa
    42481 gttaagcatc attttgatga taaagaagtt gcaaatgagt gtttaagtaa gatgtagcat
    42541 aaaaattaaa caaaatacaa gaacaaaaat gtaatatcaa gcttcacttt gatattacat
    42601 ttttttatat gtaaataatt gtaaaataga taatgcaata atataaacac taatatctat
    42661 attaattaat ttattgttgt aataaagttt ataattttgt ataattacag taaatatgga
    42721 aaaataagta ttattataaa atgtttacaa taattgtcat aagataatga ttatacatac
    42781 atagattcga gaaaggcagg aagcataatg ttaagtgatg caaaaaataa taggtatggt
    42841 ttgtccataa ttgctgtcat aagtatctta gtaattacaa tttcctgttt gatttatggt
    42901 tcaaggttga ataagacatt aggtgaagaa acgaatcaat atctttcaga aatagcaaat
    42961 caaagtgtca atgtgttaaa aaaacaaatt aatggagata taaagttact agagtcaatt
    43021 tcaatattta ttgaaggaga agagtctttt gaagtagata atatcctttc tatactaaaa
    43081 agacaagcta ttaatagttc atttaaaaga atgggagtta ttttaccaaa tggtacagca
    43141 tacactacag atggttatat agaagacttc tctaaaagag attatttctt aaaatcaatg
    43201 caaggagaag tggttataac aggcaaatta aaagatgtaa tagaagatga taaaaatacc
    43261 aatagtaata ttaatgtata ttctgtacca atttataaag ataatgaggt taaaggagtt
    43321 atatttgcca cacatagtac gaagttgtat gaaaaaatac tttctgtacc tacatttaat
    43381 ggaaaaggat attcttatat aactagaagt aatggtgata ttgttttaaa tccaagcagt
    43441 aaaaacgcca actctactaa agaaaattta ttttcttaca ttggcagaag tgattcaata
    43501 tcaaaatcta gtatcaacaa tatgaagtca aatataaaaa actttaaatc aggttcatta
    43561 agttatagta tgtttggaaa aggatattat ttaagttatg ccccaatagg aatcaatgat
    43621 tggtatcttt tttctgaagt tcctaaaact gctatatctg agaaatcata tgctattata
    43681 aaacttacat tatcagcatg tgtagtcttg attataatct ttacggcatt aacaatttat
    43741 atactattta tacataaaaa gagtaaggaa aaattggaag aatttgcttt taaagataac
    43801 gttacaggta ttggcaattc aaataaattt aatcttgaag ggggaaaatt tttatcctct
    43861 catgagaaaa agaatctagt tttaatttat tttgatatag ataagtttaa gctagtaaat
    43921 gataggtttg ggtatgaaga aggagataga gtattaaagg aaatagcaga gataattaag
    43981 aatatattta aagagcaatc tgtattttca agaatttcaa atgacaattt tgctataata
    44041 tttgaaaaga aaaataatag agagtcaatt atagaaatat gtgaacttat acgtaaaaaa
    44101 ttgtcaatga taaaaacaag tttgggagta gaactcaatt taattccatc cataggtgta
    44161 tattttgttg aagagggaga aacaaatatt tctacctgtc ttgacaaagc tatgattgca
    44221 aaaactacag taaaaagaaa atatttaaaa gtttatgaga tttatgctga aaatttaaaa
    44281 gaaactctca tagaagaacg tgatattgaa caagaaatgc atgatgcttt aaaaaatgaa
    44341 caatttaaag tttatttaca gcctaaggtt gatttatcaa ctactaaaat tgtaggtgct
    44401 gaagcacttg tacgttggca acatcctaaa aaaggtctaa taagtccagg tgtatttatt
    44461 cctatttttg agaaaaatgg ctttataaca gaattggata tgtttgtatt tacccaagtt
    44521 tgtaaaaatt ttaaacgttg ggaaaatgaa aatttcccca catttcctat atcaataaac
    44581 ttatcaagag tacatcttga gaatccaggc tttatttctg agcttgaaag aataacaaaa
    44641 gaatatgaaa ttgaaccaaa tttaattgag attgaattga cagaaagtgc aatatttgac
    44701 aatacaaaaa tattatttaa aataatgcaa actttgaaat ctgttggatt taaaatttct
    44761 atggatgatt ttggttctgg ttattcttct ttaaatatgc ttaaagatat gccaattgat
    44821 gtattgaaat tagataggca attctttata acagtgggaa atgcaaaaaa gagtcaaatt
    44881 gtagtttcaa gtattgttca aatggcaaag caacttgata taaaagttgt ttcagaaggt
    44941 gttgaaactg tagagcaagc agagttttta aggtttatag gttgtgatat ggcccaagga
    45001 tttttatttg ctagaccaat gccaatagaa gaatatgaaa aattaatatt agatagatat
    45061 agtttaaata gaaaagaaac aaattaaata aaattgtgaa ttataacata aaaatttata
    45121 aaaaagtgaa ctttgttaat caatgttcac ttttttatgt aaaaatatac tattataaaa
    45181 acataaatca ttgttaggat attattttag gaattattgg gcaaagttta ttaatatgta
    45241 gtatttttta catttgaatg tttttgaggt aagagtatat gggattatag caaaatactt
    45301 tagatagtaa taatag
//
LOCUS       DAMJWS010000024        44054 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMJWS010000024 DAMJWS010000000
VERSION     DAMJWS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.024"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(121..894)
                     /locus_tag="PC399_001388"
     CDS             complement(121..894)
                     /locus_tag="PC399_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF2592261.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="PC399_001389"
     CDS             complement(894..1280)
                     /locus_tag="PC399_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2592262.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="PC399_001390"
     CDS             complement(1305..1622)
                     /locus_tag="PC399_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF2592263.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="PC399_001391"
     CDS             complement(1634..1894)
                     /locus_tag="PC399_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF2592264.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="PC399_001392"
     CDS             complement(1894..2190)
                     /locus_tag="PC399_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2592265.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC399_001393"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC399_001393"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF2592266.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC399_001394"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC399_001394"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF2592267.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKRYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC399_001395"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC399_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF2592268.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="PC399_001396"
     CDS             complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="PC399_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF2592269.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8082..9188)
                     /locus_tag="PC399_001397"
     CDS             complement(8082..9188)
                     /locus_tag="PC399_001397"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF2592270.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="PC399_001398"
     CDS             complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="PC399_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2592271.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11408..11545
                     /gene="rpmH"
                     /locus_tag="PC399_001399"
     CDS             11408..11545
                     /gene="rpmH"
                     /locus_tag="PC399_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2592272.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11623..11967
                     /gene="rnpA"
                     /locus_tag="PC399_001400"
     CDS             11623..11967
                     /gene="rnpA"
                     /locus_tag="PC399_001400"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF2592273.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11999..12250
                     /gene="yidD"
                     /locus_tag="PC399_001401"
     CDS             11999..12250
                     /gene="yidD"
                     /locus_tag="PC399_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF2592274.1"
                     /translation="MNKIIYILKEISKHLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12282..12989
                     /locus_tag="PC399_001402"
     CDS             12282..12989
                     /locus_tag="PC399_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2592275.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12958..13587
                     /locus_tag="PC399_001403"
     CDS             12958..13587
                     /locus_tag="PC399_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF2592276.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13699..15078
                     /gene="mnmE"
                     /locus_tag="PC399_001404"
     CDS             13699..15078
                     /gene="mnmE"
                     /locus_tag="PC399_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF2592277.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENVIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15176..17068
                     /gene="mnmG"
                     /locus_tag="PC399_001405"
     CDS             15176..17068
                     /gene="mnmG"
                     /locus_tag="PC399_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF2592278.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17061..17780
                     /gene="rsmG"
                     /locus_tag="PC399_001406"
     CDS             17061..17780
                     /gene="rsmG"
                     /locus_tag="PC399_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF2592279.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17943..18728
                     /gene="noc"
                     /locus_tag="PC399_001407"
     CDS             17943..18728
                     /gene="noc"
                     /locus_tag="PC399_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF2592280.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18863..19636
                     /locus_tag="PC399_001408"
     CDS             18863..19636
                     /locus_tag="PC399_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2592281.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19638..20501
                     /locus_tag="PC399_001409"
     CDS             19638..20501
                     /locus_tag="PC399_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2592282.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20534..21676
                     /locus_tag="PC399_001410"
     CDS             20534..21676
                     /locus_tag="PC399_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2592283.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="PC399_001411"
     CDS             complement(21810..22385)
                     /locus_tag="PC399_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF2592284.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /locus_tag="PC399_001412"
     CDS             22645..23937
                     /locus_tag="PC399_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2592285.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="PC399_001413"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="PC399_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF2592286.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="PC399_001414"
     CDS             24717..24980
                     /locus_tag="PC399_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF2592287.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="PC399_001415"
     CDS             24995..25180
                     /locus_tag="PC399_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF2592288.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="PC399_001416"
     CDS             25283..26479
                     /locus_tag="PC399_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2592289.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /locus_tag="PC399_001417"
     CDS             26693..26971
                     /locus_tag="PC399_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF2592290.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /gene="ssb"
                     /locus_tag="PC399_001418"
     CDS             27008..27442
                     /gene="ssb"
                     /locus_tag="PC399_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2592291.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /locus_tag="PC399_001419"
     CDS             27461..27688
                     /locus_tag="PC399_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF2592292.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="PC399_001420"
     CDS             27831..28151
                     /locus_tag="PC399_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2592293.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="PC399_001421"
     CDS             28159..29115
                     /locus_tag="PC399_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF2592294.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="PC399_001422"
     CDS             29126..31120
                     /locus_tag="PC399_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF2592295.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /locus_tag="PC399_001423"
     CDS             31121..31570
                     /locus_tag="PC399_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF2592296.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="PC399_001424"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="PC399_001424"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF2592297.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="PC399_001425"
     CDS             33122..33430
                     /locus_tag="PC399_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592298.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="PC399_001426"
     CDS             33512..34801
                     /locus_tag="PC399_001426"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF2592299.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="PC399_001427"
     tRNA            35127..35202
                     /locus_tag="PC399_001427"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="PC399_001428"
     tRNA            35209..35283
                     /locus_tag="PC399_001428"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="PC399_001429"
     tRNA            35288..35363
                     /locus_tag="PC399_001429"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="PC399_001430"
     tRNA            35397..35471
                     /locus_tag="PC399_001430"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="PC399_001431"
     CDS             complement(35854..36846)
                     /locus_tag="PC399_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2592300.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="PC399_001432"
     CDS             complement(36855..37913)
                     /locus_tag="PC399_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2592301.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="PC399_001433"
     CDS             38038..39501
                     /locus_tag="PC399_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF2592302.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="PC399_001434"
     CDS             39603..40400
                     /locus_tag="PC399_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2592303.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="PC399_001435"
     CDS             40413..42515
                     /locus_tag="PC399_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF2592304.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="PC399_001436"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="PC399_001436"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF2592305.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43211..43522
                     /locus_tag="PC399_001437"
     CDS             43211..43522
                     /locus_tag="PC399_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2592306.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43570..43887
                     /locus_tag="PC399_001438"
     CDS             43570..43887
                     /locus_tag="PC399_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2592307.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
BASE COUNT        16623 a         5541 c         6996 g        14894 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ctttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa atacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttaacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat ctctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 acaatacttt tattttagtt ccttctattt caactatatc tcctgatttt atcttttttc
     7801 ctcttcttaa ttctatttca tcatttactg tcactaaacc ttcttgaatt aaaaattttg
     7861 catgacctcc tgttgatgca atctctgcta atttaagaaa ttgatccaac tttatatatt
     7921 ctgattctat agttatttca gtcataatag ttcctccttg tcactatttt aaacaaattc
     7981 ctattgatac actaaaagta tattatacca taaatatgct tataaaagga gttgtctaag
     8041 attagaaaaa ttttctacaa aagacaactc cttcatgtct gctatatatt tgatgatatt
     8101 cttactggaa gcagtaagta aatataatta acatcatctg ttggtttaat tatacaagga
     8161 tttacatttg tagtaaattc tataaatatt tcctcattat ctatattttt caaaccttcg
     8221 ataaaatatc tagaattgaa agctatatct aggtaatctc catctaaatc tatttcaact
     8281 tcttcataca catttccttt ttctgtatta gaagtaattg ccattacttt atctcttata
     8341 gataacttta taagattatt tttttctgat tgagacaata aagacgctct ctctatacta
     8401 tttaaaagtt ccttagtatt taatttaact ctgctgttat gttctcttgg caataatttt
     8461 ttataatcaa taaaatctcc ttcaagcaat cttgtaataa ttttcgtatc attaattata
     8521 aaaatagcat ttttttcgtt aaatccaact ttaacattat cctctccaga aagcaaacta
     8581 tttacatcta taagtgtctt tccaggaatt atgactttta tattttcagt taaactatca
     8641 accgaacaac tttttacagc taatctatat ccatcaattg ctactaaatt taagtttcta
     8701 tctacaattt ctaaaagttc tcccattaaa acaggttttg tttggtcctg tgagatagca
     8761 aatacagttt gtttaatcat attttttagg atttcttgag gtatgctata taaatcttct
     8821 tcatttaatt ctggtaattt aggaaattct tttgctgcat aacctttaat tttaaatctt
     8881 gaatttacac aatttatgta aatattattt tctgaatcag tttctatctc aacaaaggaa
     8941 tctggtaatt ttctaattat atctccaaat agccttgcat ctacaacaac atcacctttt
     9001 tctatgattt ctgcttgtac ataagtttct attcctattt cagcatcata tccagtaagt
     9061 tttaattgac cttcttctgt agatataagt atacctttta ataattctat tgttgttttt
     9121 ccattaatag ctttttgagc aattccaatt ctatttgcta ggattttttg attacaaatt
     9181 attttcaatg tggataacct cctctggctt tttatatata aaaataagtt aaaatcatag
     9241 tagtaatagt agtaggtgtt gttgataatg ttaataagta tgtataacat tataaataca
     9301 aagatattaa catgtgtata agatgttaat atctttgtta ttttagactt gataatttgt
     9361 taataatttt tgttcaaaaa aagtattaac aggttattca agagatataa acatacaatt
     9421 gtgtataaat tatcccttca aatctgatat aattttgtct attttagtct taatttcttc
     9481 actttcttct atatccttag atactttgtc atgagcatga attactgttg tatggtctct
     9541 acccccaaac tcttcaccta ttttaggaag ggataaatca gtaagctcgc gcgttaaata
     9601 catagctatc tgtcttggat aagctattga acgtgttctt tttttagaat taaaatcttc
     9661 catctttaaa ttgaatacag aagatacttt ttcttttatt ctgagaacat ttatttcttc
     9721 atttttagaa gttgttataa tatcttttaa agcttctgta gcaagatcaa aagatattac
     9781 cctattgcta agagaagaat aagcaaccac tctagttaat gcaccttcta gttcccttat
     9841 gtttgactta atattttttg ctatatatga cataacctca tttggcacat ctatacgttc
     9901 tagttgagcc ttttttctaa gtatagcaat tcttgtctca aagtcaggtg cttgaatatc
     9961 agttataagt cccatttcaa atctcgatct tagcctatct tctagtgtag gtatgtcttt
    10021 tggcggtcta tcactagata taattatttg tttgtttgct tcatgaagag tattgaatgt
    10081 atggaaaaac tcttcttgag ttctttcttt tcctgctata aattgaatat cgtcgattag
    10141 taatacatct acgtttctat atttattcct aaattcttcg tttttatcgt cttttataga
    10201 gtttataagt tcattagtaa atttttctga agaaacgtaa actacttttg agtctttttt
    10261 ttgactaaca atatgatgac ctatagcatg cattaggtga gtcttaccta atccaactcc
    10321 tccatacaaa aaaagtgggt tataagcttt agctggagat tctgcaactg caacgcatgc
    10381 agcatgtgca aatctattac tattaccaat aacgaatgta tcaaatgtgt attttggatt
    10441 tagattgggg taaagaactc tataatttaa atctgtgcta tcagaattta aatcagcaac
    10501 ctctttttcg ctcaatacaa atttaatatt atattttttt aatgacagtt gactaatagc
    10561 atcttcaatt aaatgtaaat atctattctc taagatatct ttattaaaat cactaggagc
    10621 cagtaagatt aaatcattaa gatgtatttt taatggtacg atgtttttaa aaaaggtatt
    10681 aaaacttact gaagttaagt caccttttat taattgtagg gttttgtccc ataaagaaac
    10741 tatatccata atataaacct ccgaacagaa taattaatat tttccacaaa gttattaaca
    10801 gaaaagcaaa aatatatata aattttacag aataactaaa tttatggata atggtgttga
    10861 taaattaata atagtataaa ttattaaaaa aatatgaaaa gatataaaca atatgtagat
    10921 aaagttataa ataacataaa catacaataa atggtgatta taactagagt attgtcagta
    10981 atcttaaaag ttatacacaa aagtatccac aagctgtgaa taatttctaa taagttgata
    11041 acatttatgc acattatcaa caagtatatt tataatatca aaatatatgt caaacttcaa
    11101 tgatttgtaa ctaatttatc cacaatatta actaattgtt gataaaaata taaacatgtt
    11161 aaaattactt tatttgtgta tataattgaa aaaaataatt tagaataaaa ttaaatagat
    11221 ttttactagt attttcaata aaaaaaatac ataatatata tatggtagat tttgtgatat
    11281 aagcgtccca aaaactcgtt gacaacagta gtaaaagtat ttataattat atgtgttatg
    11341 tttactttga gggtaaagtt attattttta taaacggaaa ataaaatctg aaaggcggtg
    11401 caaaataatg agtaaaagaa cttatcaacc aaaaaagaga caaagaagta aagaacatgg
    11461 tttcagaaaa agaatgaaga cgtctaacgg aagaaacgtg ttaaagagaa gaagagctaa
    11521 aggaagaaat agattaactc attaattaca aaagggccgc tgcaaaggtg gcctttttgt
    11581 gtaaataatt acaattcata gctgtgttta tggaggttaa ctatggactt taataggact
    11641 aaagggttga aaaaagactc tgattttaga aaagtatata aacacggcaa atcttttgca
    11701 aacaaatatt tagtaatata tatacttaaa aataaatcag attatagtag agtaggtatt
    11761 tctgtttcaa aaaaagtagg aaaagctatt actaggaata gagtaagaag attaataaaa
    11821 gaagcctaca gattaaatat tgatgaaaaa attaagcctg gatatgatat agtatttata
    11881 gcaagggtat ctagtaagga tgctactttt aaagacatag acaagtctat aaaaaattta
    11941 gttaaaagaa cggatatttc tatttaattg tatgctataa atatggaggt taatgacttt
    12001 gaataaaata atttatatat taaaggaaat aagtaagcat ttatcgaatt tatgtattta
    12061 tttagtgaga ttttatcaaa agtatatatc tcccttaaag ggacctacct gtagatttta
    12121 tccaacttgc tcacaatatt caatagaggc gtttaagaaa tatggatttg taaaaggtat
    12181 gtatttaacg ataagaagac ttttaaaatg tcatcctttt catccaggag gttacgaccc
    12241 tctaaaataa aagagagtaa ctattaggag gtataaaaaa attgaatata ataggaaatt
    12301 tcttaggatt gattcttaag gtaatatttg aatttgttaa tcattatggt atttctataa
    12361 tactttttac gatacttgta aagattattt tattaccatt aacaattaag cagactaagt
    12421 caacaaaggc aatgcaagat atacaacctc gtataaaaga gatacaagaa aaatataaaa
    12481 ataagccaga aaaacaaaat gaagaaattg ttaagttata tggagaagct aaaattaatc
    12541 cactatctgg ttgtttacct ttattaatac agtttcctat attaatagga ttatttagcg
    12601 ttttgagaga accagttgct catggcgttt ttgcaaacaa agctgcattt ttagcagctg
    12661 acaatgggtt tttatggata aaaagcttaa catcaccaga ttatgtatta gcagtatttt
    12721 caggagcaag tgcatatgtc atgcaaaaag taatgacacc aaaagatcag ttacaaggct
    12781 ctatgaaagt aatgacatat gtaatggcag gtatgtcttt ctattgggga tttatattcc
    12841 cagcaggact tacattgtac tggacagtaa gtaacttatt ctcaatagct caatattact
    12901 taataatgaa tcctttaaaa gctaaactgg ctgctaactc taaggaggaa aaagttaatg
    12961 agaaaaagcc taaaattaat aaaaagtaag agtaaagagg aagctataag taaagctata
    13021 gcagagttga atttaaaagc agaggatata gaagtagaaa tacttgaaaa tccaagtaaa
    13081 gggtttttag gttttatagg tgctaaagat ggtacttatg aaatttttgt tatcgagagg
    13141 gaagtagatg tagctaaaaa ttttatagag gtaatgttga aaaatgcaaa tgttgatgcc
    13201 aaagtcaatg tatctcaaaa agataattta atcatggttg atattgaagg caaagaagct
    13261 gcaagtttaa ttggtagaag aggggaaact ttagattcta ttcaattttt gactggatta
    13321 gctcttaata aaataaataa agattctcat acaagagtac ttgtagatat agaaaattat
    13381 agatcaaaac gagaagaatc tttaataaga tatgctaata aagttgctag agaagtagcg
    13441 aaaacaagaa aaactaaaaa attagattat atgaatccat atgagagaag aataatacat
    13501 tcagctcttc aaaatgacaa atatgtaatt acatatagtg aaggaaccga tccatataga
    13561 agattagtaa ttgagtgtaa aagataaagt caacctccta aacattaggg ggttttattt
    13621 tttaatatta ttaaatcatt aatggttggt taaatgtaaa tctatatgta aaaaattatg
    13681 aattaagagg tgataaattt gtttatagat gatacaattg cagcaatagc tacagcacct
    13741 ggagaaggtg gtataggtat acttagaata agtggagaaa aagctttgaa ggttgcagaa
    13801 gaaatcttta aatctatgtc tggaaagagt attgaagaat acaataagag aacattaata
    13861 tatggaaata tagtggataa tgaaaatgta attgatgaag ttttattggc atatatgaaa
    13921 ggaccaaatt cctatacagg agaagatgtt atagaaatta attgccatgg tggatttatt
    13981 tcggtaaaga agatattaga attaatacta tcaaaagatg ttagattagc agaggctggt
    14041 gagtttacta aaagagcatt cttaaatgga agaatagatt tatctcaagc agaggctgta
    14101 attgatgtta taaaagccaa aactgatata gctcatgaag ttgctcaaaa tcaattagag
    14161 ggttctttat caaagaagat aagagaattg agagataaag tcactgagat attagctcat
    14221 gtagaagttg ctatagatta tcctgaggaa gatattgagc atataacata tcaaactctt
    14281 aaagaaaaaa ctgatgaact taaaaaagac attaaaaaat tatatgatac agctgaaagt
    14341 ggaaaaatac ttagagaagg cttaaagacg gtaattgttg ggaaaccaaa tgtaggtaaa
    14401 tcttcactac ttaattctat tttaggagag aatagagcaa tagttactga tataccagga
    14461 actactagag atgttataga agagtttgta aacataaaag gtataccttt aaaaattgtt
    14521 gacacagcag gtataagaga tactgatgat attgttgaaa aaataggtgt tgaaaagtct
    14581 aaagaatcat ttactagtgc agatttgata gtaatggttt tagatgcatc tagaaagttg
    14641 tcagaagaag atatagagat attagaaaaa cttaaggata aacaaacaat agtgctgtta
    14701 aataagaatg atttgaaaca agagattgaa gaagaaaaaa tacttaaata tgtagaaaat
    14761 aatagtataa taaaaatatc agctttacaa caagaaggaa tagaagaatt gcaagataaa
    14821 atagagtcta tggtatataa aggtagtatc aaaaataatt ctagtttagt agtaacaaat
    14881 tcaagacata aagatgcact tagtaaagca tataaatctg caacagatgc tttaattgct
    14941 ttggaacaaa gtatgccatt tgactttgta gaagtcgatt taaaaaatat atgggactat
    15001 cttggatata ttaatggtga tacagtaaca gaggatttac tagataatat attccataat
    15061 ttctgtattg gtaagtaatg tacaaaagta aacttgtttt tattatagag atttttaaaa
    15121 aatttgaagt gtttctcatg aaacattaat tttgaaaaga aaggaattat aaatgataaa
    15181 gtttgaagca ggaaaatatg atgttatagt agtcggagca ggacatgctg gatgtgaagc
    15241 tgctttggct actgcaagaa tgggatataa aactttgata ataacaatgt ctttggattc
    15301 catagcatta atgccttgta atccatcaat tggagggact ggtaaaggtc aattagttaa
    15361 agaaatagat gccttaggcg gtcagatggg attaaatata gacaagacat atatacaaag
    15421 tagaatgtta aatactgcca aaggtccagc ggtacactct ttaagagcac aagcagataa
    15481 atttaaatat catgaagaaa tgaaaaagac attagaagat gaaccaaatc ttgatatagc
    15541 tatggatgaa gttgtagaaa tacttcatga aggaaatgtt gtaataggag ttggaactaa
    15601 actaggatgt tcatttaaat ctaaagctgt aatattggcc actggagttt atctgaactc
    15661 aaagatatat atgggtgaag ttgcatttta tgaaggtcct aatgcattag ggtatgcaaa
    15721 atatcttaca gatagcttag ttgagcttgg ccttagaatg agaagattta agacgggaac
    15781 tccagcaaga gttcatagag atagtataga cttctctgta atgtctttac aagagggtga
    15841 tgaaaaagta actccattct catttatgaa tgaaaatata gaaaaaaaac aagaaccttg
    15901 ttatttaaca agaactacag aagaaactca aaaagttata ctggataatt taaaaaggtc
    15961 tgctatgtat agtggagtaa tagaaagtac tggtccaaga tactgtcctt ctatagaaga
    16021 taaagtagta agatttagtg ataaaacatc acatcaatta tttatagaac cagaagggtt
    16081 aaatactaaa gaaatgtata tacaaggaat ttcaactagt ttaccttttg aagttcaatt
    16141 agatatgtac aaaactataa aaggacttga aaattgtaag ataatgagac ctgcatatgc
    16201 tattgaatat gattgtgttg acccaactca actaaaaata agtttggaaa taaagggtgt
    16261 tgagaactta tttagtgcag ggcaatttaa tggaacttct ggatacgagg aagcagcagc
    16321 tcaagggctt atggctggta taaatgcagt tagaaagata gaaggaaaag agccatttgt
    16381 attagaccgt tcagaagctt atataggtgt cttattagat gaccttgtta ctaaaggaac
    16441 taacgagcct tacagaatga tgacatcaag agcagaatat cgactatatt taagacaaga
    16501 taatgcagat atgagattaa ctcaaaaagg ttatgatata ggattagtta aaaaagatag
    16561 atatgaaagg ttcctaaata aaaaggctgc tgtagagaaa gaatttgaaa gactaaaaaa
    16621 tgaaagagtt acaccaaaag aagtaaatag tttgttggaa gaaaaaggag caacaccaat
    16681 aaaggttgga atatctctat atgaattttt aaagagacca gaggttactt acgaattgct
    16741 tgaagaatta gggaaaggcg ctggagaaga tgtttcaaga gaagttaaag aacagtgtgt
    16801 aataataact aaatatgaag gttatataga gaaacaattg aaacaaatag accaatttaa
    16861 aaaacttgag aataaaaagt tggatgagaa aataaattat tcatctatag aaggtcttag
    16921 attagaagct agacagaaat tagatgatat aaaacctatt tcaatagggc aagcatctcg
    16981 tatatctggt gtttctccag ctgatatatc agttttactt atatacctag aacaaatcag
    17041 aagaactaga ggtggcaaag gtgaataata tagaacttct gaaaaacggg attgaaggtt
    17101 ttaatataga tacgaatgac agtatgttag aaaaatttaa aatctataga gagatattag
    17161 ttgaatggaa taaaaagatg aatcttactg gaatagaaga tgaaaaagaa gtgtatataa
    17221 aacatttttt agactctatt tcagctgtaa aaaatggata tataaaaaat gggatgtcta
    17281 taatagatgt tggtacaggt gctggttttc ctggaatacc attgaaaata tgtttagata
    17341 gcttagaact tactttgtta gattcgttaa ataaaagaat aaacttttta gaggaagtta
    17401 gtagagtact agagttagat aatattacat ttatacatgg aagagctgaa gattttggta
    17461 aaaatgaaga ctatagggaa aaatatgatg ttgctacggc aagagctgtt gctggattac
    17521 cgattcttat ggaattttgt gtaccatttg taaaagttgg agggtatttt atttgcttaa
    17581 aaggtccgaa tgctaattta gagctagaag aatcacaaaa ggctattgat gttttaggat
    17641 tggagtatat tgaaaaaata gatgtagagt taccagaggt agatttaaat cataatatat
    17701 tagtttttaa aaaagtgatg gaaacaccag ttaagtaccc tagaaaagct ggtaaaccag
    17761 ccaaaaatcc tattaagtaa tgaaaaattt attattggta agcttaatta tttctcagga
    17821 aataattaag cttactgtaa aaaaacaaaa ataaaggaat tattaaagta gatgcagaaa
    17881 taaatttgta tgtacatata gagaacaaaa ataatttata tctaaaataa gagaggtatg
    17941 ttatggaaga taaaagagtt atggagatac ctatagaaga tgtagttcca aatccatatc
    18001 aaccaagaaa aatattttca caagtttcat tggaggaatt gagcaattct ataaaggtat
    18061 atggtataat tcaacctata acagttagag caaaagatgg caaatatgaa ctaattgcag
    18121 gagaaagaag attaagagca gctaaattag cagagcttaa aaccatacca gcaattataa
    18181 ataatatgaa cgatgaatct tctgctgttc tagcgttatt agaaaatttg caacgagaag
    18241 acttaaattt cattgaagaa gcaattggtt atgaaaactt aataaaagaa catgctttta
    18301 ctcaacagca attagcagaa aaattgggca aaaatcaatc tactgtagca aataagctta
    18361 gaatattaaa gcttccaaac gatataaaaa tgaagctaat agagaataat ttaacagaaa
    18421 gacatgctag agcatttctt aaactaccaa gtgaagattt aatgcaaagt gtgttagaca
    18481 aagtcataaa aaatgaattg actgttaaaa aaacagaaaa attgatacaa gatgtattag
    18541 aagaaactaa agttcaagaa gagcctgata aaaaacagaa tataaagggt gctatgagta
    18601 taagaattta cataaatact ataaagcagg cctttgatgc tatatcaaat acaggaattg
    18661 atgctaagta taatgaaata gataagggag attatatgga agttgttgta aaaatcccta
    18721 aaaaatagag attactgctt accagtaatc tttttttgta atataagcct tgtaatataa
    18781 attgatttat agacattatt tatacttaaa aaacacctat tcaacattta aaattaattt
    18841 aattcttaaa ggggggaaca tcatgggcaa agttatagct gtattcaatc aaaagggtgg
    18901 agtcgggaaa actacaacaa atgtaaattt aagtgctagc ttaggaactt tgggaaagaa
    18961 aatattagta ttagatttag acccacaagg aaatacaact agtggatatg gtattaataa
    19021 aaacgaagta gaaaatacaa tctatgaaat tatgttagat gggttacata ttaaagaagc
    19081 aataataagt actgaatttg agaatgtaga tgtagttcca tcagctactg aactatcagg
    19141 tgcagagata gagcttacat ctaaaactaa tagagagtat attttaaaaa attccataaa
    19201 agcggtaata gatgaatatg attatatatt tttagattgt ccaccctcac tggggatgct
    19261 tacaataaat tgtttaactg ctgtagatag tgttttaatt ccaatacaat gtgaatatta
    19321 tgctttagaa ggtgttagtc aattaatgga gactataaaa ttagtaaaat caagattaaa
    19381 tgctgatata gaaattcaag gtgttgtttt aagtatgttt gatggaagag ctaatttatc
    19441 aatacaagta gttgaagaag ttaagaaata ctttaaaggt agtgtatata caactttaat
    19501 tccaagaaat gtaagacttg cagaagctcc aagtcatgga aaacctgtaa tttattatga
    19561 taaaagatgt agagggtctg tagcttattt ggaactagca gaagaattta tagatttaga
    19621 agaggaggaa tggtagtatg gagaataagt ctaaaagaag taatagatta ggtagaggtc
    19681 ttagtgcatt gataccagaa attaaaggag aaacttcaga aaaagaaata gtcaacatag
    19741 atatagacaa aatatatcca aacgaagttc aacctagaaa gcaatttgat gaagaaaaga
    19801 taaaagtatt gtcggattct ataaaaaatt atggtgtttt acagccaata gtggttaaaa
    19861 tggatgaaaa taataaatat atgataatag ctggagaaag aagatttaga gcatcaaaac
    19921 ttgcaaataa aaatcaaata cctgctataa ttaaagatat agatatgaaa gatataatgg
    19981 agatagcctt aattgaaaac ttacagaggg aagatttaaa ttcaatagaa gaggctctag
    20041 catataagag tttaatagag cattacaatg taactcagga agaaatatca gaagctgttg
    20101 ggaagagtag acctcatata actaatacac taagactttt aaatcttgga caagatgtta
    20161 tagaaatgat agacagtggt agaattacag caggccatgg taaggcttta ttgagaattg
    20221 cagataaaga tttgcaactt caaatagcga aaaaaataga agaagaagaa ctttctgtaa
    20281 gagaagtcga aaatatagct aaaaaaatat ctgaaaataa acaagaagaa cctaaaaaat
    20341 ctaaacctaa ggatgtattt attttagatg ttgaagataa acttagaaat atatttggaa
    20401 caaaggtgaa tatttctaaa ggaaagaaaa aaggtaaaat agagatagaa tactataatg
    20461 atgatgattt aaatagtatt gtatcaatgc ttcttgaata gaacaaaaat ataaaacaga
    20521 gaggaagatt gttatgattt atttagataa tgctgcaact acatatccaa aaccagaaag
    20581 agtgtataat gctgttttag attgtatgaa aaattattgt gcaaatccag gtagagcagg
    20641 ccataaactt gctatgagag ctgctaggga gatatatgat acaagagaga atatagctaa
    20701 gttgttcaat gtaagtaatc ctatgaatat agtatttaca tctaatgcaa cagattcttt
    20761 aaatttagcg ataaaaggtg tattacaaga aggagaccat gtaataacaa cgtctatgga
    20821 gcataattca gtaataagac ctataaaagc attggaaaaa aggggtattg aaaatactgt
    20881 agtaaaatgc gattatgagg gatttttaga ttatgaagat ttagaaaaat caataaagaa
    20941 taatactaaa ttgattgtaa caacacatgc gtctaatgta tgtggaactt taatagatat
    21001 aaaaaaagtt ggagaaatag ctaaaaaaca taatatacta tttttagtag atgcatcaca
    21061 aacagcaggt gtttatgata tagacgtaaa tgaatgtaat atagatatgt tggcaatgcc
    21121 aggtcataaa tgcttatttg gacctcaagg aacaggtata ttatatgtaa gagaagggtt
    21181 gaacttaaat atattaaaag aaggtggaac aggtagtaaa tcagaagaaa tagttcaacc
    21241 agagttattc ccagataaat atgaatcagg aactcataat actccaggta tagcaggtct
    21301 taaccaaggt atactattta tatttgaaag aggaatcaac aatataagac aacatgaaga
    21361 agaattatgt caatatatga tagacaaatt agaggaagta ccagatataa agatatatgg
    21421 accaaaggac agtaaaaaaa gagcatcagt aatagctcta aatataggag atatggattc
    21481 tggagaggtt actttcttat tagatagtga ttataatata gcgactagat caggaataca
    21541 ttgttcacca ctagcacata ccactttagg tactttaaaa caaggtgcag taaggttcag
    21601 tataggatat ttcaacacaa aagatgagat agataaagca gtggaagctt tgaaaaaaat
    21661 ttcaaaaaat aaatagcatg agatttaata atatagaaat aaaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggttc ctaagaacct
    21781 ctaatctatt tgataatagt ttcaagaaat tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 ggtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagagtaa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa atttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtatttat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttatg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattaaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaacca ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagaatct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaata taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagagcaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt atagggccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttga cttacattga ttttttatta agttaaagct
    34921 acttatacat attctaactg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 ataatattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taaggtacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag attctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aggtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcacttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacttt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tcttcattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag aaacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacataga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagctga aaacgttaga gattttgcat ttatactaag tagtaaattt
    38821 gatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagtga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcttt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aatagtgaat gtaagtaaat tttgtgcaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aaaaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttaa
    40261 gcaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aactgctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtggtaatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac cgttacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt taagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gagcttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctttagggct ttctgatgag gttttaggaa gggctgatta taggttatcc
    42961 ttttctaaga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gtttttgtta
    43081 atttgacact taataatatt aaaataacaa aaaagtattg aaagtatatt gcatttaagg
    43141 tagaattaaa taaaatatta taacattata atgtttatat ggttgttaat aaaaatttgg
    43201 aggaaaatat atgataaaaa taatgttggc ctgttcagca ggaatgtcta caagtttatt
    43261 agttactaaa atggaaagtg cagctaaaga aaatggtata gagtcgcaaa tttgggcaat
    43321 accagaatca actatacaaa atgaaataga aaaatgtgat gtattattac taggaccaca
    43381 agttagatat gtattaccta aggctcaaga aattgctaag ccatataata taccagtaga
    43441 agttataaat atgatgcact atggaacagt taatggagaa gctgttttaa atagagcgat
    43501 agaacttaac aacactaagt aatataaatt ataatgtatt tttaatatat aaattgggag
    43561 aatcaaagga tggatgaaaa gactatagaa atatctttta atataatagg ttatgcaggt
    43621 gaaggtaaag gattagcttt tgaagcaata aaagaggcta agaatggaaa tatagaaaaa
    43681 gcaaaagaat tattaaaaga gtccagggaa gttataaata aagcacatag atatcaaaca
    43741 gaattaatac aaaacgaagc tagtggaaac aggactgaaa taagtgtcat acttatacat
    43801 gcacaggacc acttaatgaa tggcatgaat tttcaacagt tagctgagga aatcgttgat
    43861 ttacatttga aattacaaga taaataaaaa ttaaagataa taaattaaga gagttcttaa
    43921 aagatacatt atgtgtagca cactaattgt tagattattg attcaatatt tagtgagtaa
    43981 tacattagga ttattttgag gactcttttt atatttagtt taaagttaat aaatatttat
    44041 tattaataat ataa
//
LOCUS       DAMJWS010000025        43286 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMJWS010000025 DAMJWS010000000
VERSION     DAMJWS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.025"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            222..686
                     /locus_tag="PC399_001439"
     CDS             222..686
                     /locus_tag="PC399_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2592308.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            693..1187
                     /locus_tag="PC399_001440"
     CDS             693..1187
                     /locus_tag="PC399_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2592309.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1203..2228
                     /locus_tag="PC399_001441"
     CDS             1203..2228
                     /locus_tag="PC399_001441"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2592310.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2215..4662
                     /locus_tag="PC399_001442"
     CDS             2215..4662
                     /locus_tag="PC399_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2592311.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4842..6215
                     /gene="radA"
                     /locus_tag="PC399_001443"
     CDS             4842..6215
                     /gene="radA"
                     /locus_tag="PC399_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2592312.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6218..7288
                     /gene="disA"
                     /locus_tag="PC399_001444"
     CDS             6218..7288
                     /gene="disA"
                     /locus_tag="PC399_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2592313.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7377..8471
                     /locus_tag="PC399_001445"
     CDS             7377..8471
                     /locus_tag="PC399_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2592314.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8600..10777
                     /locus_tag="PC399_001446"
     CDS             8600..10777
                     /locus_tag="PC399_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592315.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KETYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TIYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWYIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10963..11808)
                     /locus_tag="PC399_001447"
     CDS             complement(10963..11808)
                     /locus_tag="PC399_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2592316.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            12025..13191
                     /locus_tag="PC399_001448"
     CDS             12025..13191
                     /locus_tag="PC399_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2592317.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYRVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYVCGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            13213..14859
                     /locus_tag="PC399_001449"
     CDS             13213..14859
                     /locus_tag="PC399_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2592318.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            14956..16329
                     /locus_tag="PC399_001450"
     CDS             14956..16329
                     /locus_tag="PC399_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2592319.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            16774..17727
                     /locus_tag="PC399_001451"
     CDS             16774..17727
                     /locus_tag="PC399_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2592320.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17765..18727
                     /locus_tag="PC399_001452"
     CDS             17765..18727
                     /locus_tag="PC399_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2592321.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18752..19738
                     /locus_tag="PC399_001453"
     CDS             18752..19738
                     /locus_tag="PC399_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2592322.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            19751..20797
                     /locus_tag="PC399_001454"
     CDS             19751..20797
                     /locus_tag="PC399_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2592323.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            20964..22694
                     /gene="lpdA"
                     /locus_tag="PC399_001455"
     CDS             20964..22694
                     /gene="lpdA"
                     /locus_tag="PC399_001455"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2592324.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            23112..26060
                     /locus_tag="PC399_001456"
     CDS             23112..26060
                     /locus_tag="PC399_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2592325.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            26038..26502
                     /locus_tag="PC399_001457"
     CDS             26038..26502
                     /locus_tag="PC399_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2592326.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            26615..26917
                     /locus_tag="PC399_001458"
     CDS             26615..26917
                     /locus_tag="PC399_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2592327.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            26979..28343
                     /locus_tag="PC399_001459"
     CDS             26979..28343
                     /locus_tag="PC399_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2592328.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFASVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            28378..28851
                     /locus_tag="PC399_001460"
     CDS             28378..28851
                     /locus_tag="PC399_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592329.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            29023..29673
                     /locus_tag="PC399_001461"
     CDS             29023..29673
                     /locus_tag="PC399_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2592330.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            29847..30779
                     /locus_tag="PC399_001462"
     CDS             29847..30779
                     /locus_tag="PC399_001462"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2592331.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            31053..31766
                     /gene="ispD"
                     /locus_tag="PC399_001463"
     CDS             31053..31766
                     /gene="ispD"
                     /locus_tag="PC399_001463"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF2592332.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            31763..32248
                     /locus_tag="PC399_001464"
     CDS             31763..32248
                     /locus_tag="PC399_001464"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF2592333.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    32343..32551
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            32615..34330
                     /locus_tag="PC399_001465"
     CDS             32615..34330
                     /locus_tag="PC399_001465"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2592334.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            34452..35897
                     /locus_tag="PC399_001466"
     CDS             34452..35897
                     /locus_tag="PC399_001466"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2592335.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            36337..37818
                     /locus_tag="PC399_001467"
     CDS             36337..37818
                     /locus_tag="PC399_001467"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF2592336.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            38039..38194
                     /locus_tag="PC399_001468"
     CDS             38039..38194
                     /locus_tag="PC399_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592337.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            38339..39736
                     /locus_tag="PC399_001469"
     CDS             38339..39736
                     /locus_tag="PC399_001469"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF2592338.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            39721..40134
                     /locus_tag="PC399_001470"
     CDS             39721..40134
                     /locus_tag="PC399_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF2592339.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            40240..40998
                     /locus_tag="PC399_001471"
     CDS             40240..40998
                     /locus_tag="PC399_001471"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF2592340.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            41011..41748
                     /gene="rlmB"
                     /locus_tag="PC399_001472"
     CDS             41011..41748
                     /gene="rlmB"
                     /locus_tag="PC399_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF2592341.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            41751..42278
                     /locus_tag="PC399_001473"
     CDS             41751..42278
                     /locus_tag="PC399_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF2592342.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            42343..42990
                     /gene="sigH"
                     /locus_tag="PC399_001474"
     CDS             42343..42990
                     /gene="sigH"
                     /locus_tag="PC399_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF2592343.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            43064..>43286
                     /gene="tuf"
                     /locus_tag="PC399_001475"
     CDS             43064..>43286
                     /gene="tuf"
                     /locus_tag="PC399_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2592344.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETP"
BASE COUNT        17739 a         4483 c         8117 g        12947 t
ORIGIN      
        1 agtatttaaa gttctgaaat ctaatcatga agcaaaaaat tagcatttgg ttatcaataa
       61 atataataaa taaaaactca taattaatga aagaataaat tcatgtctaa atttctataa
      121 aaaatttaaa tttatacaat ttagggcttt aaaaatttaa ataataggtg taaaataata
      181 gtcaaagaaa gtcaaaaata aaaaggtgtg tggtgatatt aatggcaact atgacagata
      241 taattgaaaa atttattaaa gatttgatgg aagaagataa tagcatacaa attcaaagaa
      301 atgaactagc aaacttgttt agttgtgcac catcacaaat aaattatgtc ttaacaacaa
      361 gatttacaat agatagagga tattacattg aaagtaaaaa aggtggcgga ggatatgttc
      421 aaatagaaaa aatacgaaaa agtaaagatg ggcatattag agaactacta aatgaaaaaa
      481 taggaagtca gatatcctat aaaaaagcaa aagaattatt agaaggttta aaagagtcgg
      541 atttgattaa cgaaagagaa ttaaaattaa tattatatgc aatagatgat aaatcattgt
      601 gtatgccaat atatgaatta aaagagaagg taaggtctaa tattttaaag aatataataa
      661 taggattatt tagtatagag gagtgataat ttatgctatg tcaaaaatgc aataagaata
      721 aagcatctgt atattataat aagatagtca atggagaaaa aacagagatg tatttatgta
      781 gtgagtgtgc aaaagagaat acagaaatga attttaactt agatatgcct ttttcaatga
      841 tggatatatt ttctaattta ggttttcaac ctaaaaaaga acttgaggaa aagctagttt
      901 gtcctaaatg caatacaaca tatagtgagt ttaagaataa tgggagattt ggatgtagtg
      961 aatgttataa tgcatttagt agtcaagtaa atccaatgct tcaaaatata catgggcata
     1021 tagaacatac aggaaaagca cctaaaaaat ctttttataa gataagtgta gaaaatgaga
     1081 ttaaagaact aaaagaagat ttagatagag ctataaaaaa tgaagagtat gaattagcag
     1141 ctcaatttag agataaaatt aaatatttaa aaggttctat tgactaagat tggagtgata
     1201 ttatgaaaga gaacatagtc atgaaatcaa gagttaggct agctagaaat ttaaataact
     1261 atccatttcc aaataaatta gataaagaat gtgccatgga aataatagaa aaagtaaaaa
     1321 atgcgtttat taattctaac ttagaacaaa aagaagaatt tgatttctat aaaattgaag
     1381 atttagacca atctaaaaaa atgcttatgg ttgaagaaca tataataagc ccagatttag
     1441 cagaaaatga taaaagtgca gttatagtaa aaaaagataa aactataagt ataatgataa
     1501 atgaagaaga ccatataagg atacaaacta tatgtgatga tttaaactta gaatatgcat
     1561 atagtgtagc aaatgaaata gatgatttgt tagaatcaag tttagaatat gcctttaaca
     1621 caaagctagg ttatctaaca tcttgtccaa ctaatacagg aacaggaatg agagcttctg
     1681 taatgatgca tttaccagca ttaagtcaac taggctatat ggatgaactt tataaaattt
     1741 catcacaaat aggtatagca attagaggga tatatggaga acgaacagaa gcattgggaa
     1801 atatatatca aatatctaat caacttacac ttggaagaac agagtctaat attatagaaa
     1861 atgtaagtgg acttacaaaa gatgcaattt caaaagaaat aaaagctaga gaaatattgc
     1921 aaaaaaaatt aggtataaaa ctagaagata aaatatttag atcgataggg acacttgaaa
     1981 attcaagagt aatgagtagc gcagaggcaa tgagccatct atcaaatata aaaatgggaa
     2041 tagaaatgaa ctatatagat aaactagatt taagggctat agaacaactt atgataggta
     2101 tacaaccagc gcatcagtca ataatgtata agagtgatga tgttgagaat agagatataa
     2161 atagagcaac atatattaga gaaactttgg aaaaattaag aggagggatg aattgtgaac
     2221 ttcaatagat ttacacaaag agctaaaaag gcaattgatt tagcgtttga atctgctaaa
     2281 agtctaggac acaatattgt cggaagtgaa catatacttt taggtctttt aagagaagaa
     2341 gaaggtatag ctgctaaagt tctaagtaaa gttggattta cagaagctta tttagaaggc
     2401 aaaatagttg atatggaagg taagggagaa gaaatctcag aagatatagt attaagtcca
     2461 agaagtaaac aaatacttga attatcagga atgtttgcaa ataaattaaa aacaaattat
     2521 attgggactg agcatatatt attagcaatt attcaagaag gtgaaggaat agctaataag
     2581 attttaaatt atgctggagt aaatgatagg actttagccc aattaacaat tgatatgatg
     2641 ggtattagtg ataaaaatca atacaaagca gaaaatagtt acacgggcaa ccagaatcaa
     2701 gcagaatcta aagtacttga taagtatgga agaaatctta cactatatgc aaaacagaat
     2761 aaaatagacc ctgtaatagg tagagaaaaa gaaatacaaa gagtaataca aatattaagt
     2821 agaagaacta aaaacaatcc agtgttgata ggagacccag gtgtagggaa aacagccata
     2881 gctgaaggtc tggctacaaa tatagcctta ggaaatgttc cagaaacact aaaaaacaaa
     2941 actttatact ctttagagat gggttcatta ttggctgggg ctaagtatag aggagaattt
     3001 gaagaaagaa ttaaagaagt tgtagatgaa gttgttaaaa atggaaacat aatcttattt
     3061 atagatgaga tgcacacaat aataggggct gggtctactg gagaaggctc tatagatgct
     3121 tcaaatatat taaagccagc cttagcaaga ggagaaatac aagttatagg tgcaactaca
     3181 attgatgaat atagaaagca tgttgaaaaa gattctgctc tagaaagaag attccaacca
     3241 gttatggtag atgaaccaac taaagaggac tcaataaaaa tattagaagg attaagagat
     3301 aaatatgaag ctcatcataa agttaaaata actgatgatg ctataaaggc agctgtagaa
     3361 ttatcaacta gatatatatc agacagatat ctgccagata aagctataga cttgattgat
     3421 gaagctgcat caaaagtaag attaaaagaa aatacacctc cttcagaaat aaaaaaatta
     3481 gaactagaaa tagaaaatat agataaagaa aaagaagaag cagtaagatg tcaagacttt
     3541 gagaaagcag ccaaaataag agatgaacaa ggtctactta aaaaacaatt agaagatgtt
     3601 agagaaagat ggaacaagtc atctaaacat tcagatttag ttgatggaga agttattgct
     3661 gaagtagtgg gcttatggac aggaatacca gttaataaaa ttcttgaaga agaggctgat
     3721 agacttttga gacttgagga aatactgcac aatagagtta taggtcaaga acaagcagta
     3781 aaatctattt caaaggcaat cagaaggtca agagcaggtc ttaaagaccc taatagacca
     3841 attggttcat ttttattttt aggtccaaca ggagtaggta aaacagaatt atctaaggca
     3901 ttagcagaag tgcaatttgg agatgaaaat caaataatta gaatagatat gtctgaatat
     3961 atggaaaaac atgctgtgtc aagaatgata ggttcacctc caggttatgt aggtcatgat
     4021 gaaggtggtc aattaactga aaaagtaaga agaaatccat attcagttat attatttgat
     4081 gagatagaaa aagctcatcc agatgtattt aatattctgt tacaaattct agatgatggt
     4141 agactaactg attcaaaagg aagaactgta gattttaaga atactattgt aataatgaca
     4201 tcaaatgttg gagcatctac aattggtaga caaaaaactt taggatttag tatagctaaa
     4261 ggagacgaag aagaaaaatc tcaatatgaa aaaatgaaag aaaatataat gggtgaatta
     4321 aaacaaagat tcagaccaga gtttttaaac agaatagatg atataattgt tttccactca
     4381 ctaaatgaaa atcatatatc aaaaatagta cttttaatgg cagcgaaact acaagagaga
     4441 ttaaaagaaa tggatataaa attagaaatg agtgatgagg ctgttaaatt aatttctaaa
     4501 tctggatttg acttagaata tggagcaaga cctcttaaaa gagctttaca aaaagagttg
     4561 gaagatgagc tatctgaagc aatcttaaaa ggtaatgtga aaaagggaag taatgtagta
     4621 gcaaaagtca aagatgaaaa aatagttttt gaaactaaat aatttaatag agttgcattt
     4681 taaaatattg caaatataag ttttataatc taaacaaaat tttaagggca atgaatctat
     4741 aataattcat tgtccttaat tattttattt aatctttaca tattgtcttt cagatatgct
     4801 aaaattatag tatagaataa aaaattaagg atgataaaag tatggcaaaa ataaaaacaa
     4861 aatatgtatg tcaatcttgc ggatatgaaa cagctaaatg gcttggtaaa tgtccagaat
     4921 gtacaaaatg gaatacattt gtagaagaaa tagaccaaaa aagcaccaaa aaagaggtct
     4981 ttataataga taaatcttca tctaaaccag tcagtataaa ctcaatagaa agtaaagaag
     5041 aagagcggtt tacaacagat ataaatgagt tagatagagt gcttggagga ggtatagtta
     5101 aaggctcttt agtgcttgtt ggtggtgacc ctggtatagg taaatcaaca cttttaatcc
     5161 aagtatctag taatgttgcc aatttaggaa aaactgtttt atatataact ggagaagaat
     5221 cagaatctca aataaaaatg agagctaaaa gattaggtat taactcagaa aatttatata
     5281 tatttgctga aaataattta agtataattg aatcttattt agaaagtgta aatccagaat
     5341 taataattct agactcaatt caaactgtct tcagtccaga aatatcctcg gcaccaggaa
     5401 ctgtcagcca aattaaagaa ggtacttcaa aattcatgaa aatttctaaa aaaatgggaa
     5461 tttcaacgtt catagttggg catgttacaa aagaaggttc actagcagga ccaaaattat
     5521 tagagcatat ggttgatact gttctatatt ttgaaggaga aagatataat acttatagat
     5581 tagtaagagc tgttaaaaat cgatttggtt caactaatga acttggagta tttgaaatga
     5641 gggatttagg attagtagaa cttgataatc catctaaaat tttaatctct gaaaaaccaa
     5701 aagatgtagc aggttcagta ataatatcta ctgtagaagg tacgagacca atgttacttg
     5761 aattgcaagc attagtttct cctacaagtt ttggtatacc aaaaagaaca tctacaggag
     5821 tcgattacaa tagagtaggt atgttattag cagtattaga aaaacgtgtt ggtttgcaaa
     5881 tacaaaatca agatgtctat ataaacatag ttggaggaat aaagataaat gaaccctcaa
     5941 ttgatttagg gatagccatt tctgtagcat ctagttttag aaatataccg atagatgaag
     6001 atatagctgt tacaggagaa gtgggactta caggagaagt tagagcagta agctttatag
     6061 aaaagagaat agctgagtgt aaaaaacttg gatttaaaaa gatagtagtt cctagaagta
     6121 attatgatgt tgtaaaagag accaaaggca tagaaatatg gccagtagat aacttaagac
     6181 aagctataaa tatagtactt gggaggaatc aataaatatg gagaattttc tagataataa
     6241 aaatatgcta tatgcattaa aaatgatatc tcctggaact ccacttagat taggtctaaa
     6301 caatgtacta agagctaaga ctggtggatt aattgtaatt gcaacaaacg aagatgtaat
     6361 gaaaatagta gatggaggat ttgctataaa tgcagaatat tcaccatcat atctatatga
     6421 attagctaaa atggatggag ctatagtttt aagtggtgat gtaaagaaaa tattatttgc
     6481 taatgcacaa cttatacctg actattttat agaaacatca gagacaggaa caagacatag
     6541 aacagcagaa agagtagcaa aacaaactgg tgctatagtc ataggaattt cacaaagaag
     6601 aaatgttata acagtttata gaggaaatga gaagtatgta gtcgaagata tatctaagat
     6661 atttactaag gcaaatcagg ctatacaaac tctggaaaaa tataagacag tattggacca
     6721 agctgtaaca aatttaaatg ccttagagtt taatgatttg gtaactattt atgatgttgc
     6781 attagtcatg caaaagatgg aaatggtaat gagagttaca agtataattg aaaaatatgt
     6841 gatagaattg ggtgatgaag gaactttagt aagtatgcaa ttagaagaat taatgggtac
     6901 aaccagaata gaccagaaat taatattcaa agattataat aaagaaaaca cagaaataaa
     6961 agaacttatg aaaaaggtca aaaatttaaa ttcagaagaa ctaatagaat tggtcaatat
     7021 ggcaaaacta ttagggtata gtggtttttc agaaagtatg gatatgccta taaaaacaag
     7081 aggttatagg attcttagca aaatacatag actaccaaca gcaataatag aaaacttagt
     7141 aaattatttt gaaaactttc aacaaatttt agatgcatct attgaagaat tagatgaggt
     7201 tgaaggaata ggtgaaataa gagcaacata tataaaaaat ggactcataa aaatgaaaca
     7261 attagtctta ttagatagac acatatgatg aattttaaat gaattttact acttttttct
     7321 tgaatataat atacactttg gtctataata gataagtata actagggggt gaagcattga
     7381 taaggaaagt aacaagaata ttgtttattt tacttggatt tacaataggt ataacaacct
     7441 atttaacatt aatgaaagat tttgaaatat taacatttgg aaaagaaaca tatggttata
     7501 tcgcagctgt agtagcaggt ataataatag ccattttagg atatcttata gaaccatggg
     7561 ttgtaaataa agtaaaagaa attgctaaaa tagtggataa agaattgtca aaatatcctc
     7621 agactgatat attgttaggg tctatgggtc ttatagtagg atttgttatt gcgtatttat
     7681 taagtggatt agtaaataga ataccaatag taggtggaat actatcctta ctattatatt
     7741 tattcctagg atatcttgga atgaaagttg cgcttaaaag taaaaatgat ttatttaata
     7801 ttggaaaact aggaagatta gcaaatccta ttaaggataa agataaggat aaagaaaata
     7861 aaaaagaagt aaaagccatt cctccaaaag tattagatac aagtgtaata attgatggaa
     7921 gaatagctga tatttgtaag actgggttta tagaaggaaa acttattatt cctgcttttg
     7981 ttttggagga actaagacat attgcagatt cttcagatga tttaaaaaga gtaagaggta
     8041 gaagaggtct ggatatatta aatataattc agaaagaatt aaatatagaa gttgaaataa
     8101 gtgagagaga ctttgatgat atagcagaag ttgatagtaa attattaaag cttgctcagg
     8161 ttttaaatgg aaaagttgtt acaaatgatt ataacttaaa taaagttgct caattccaag
     8221 gtgttgaagt attaaatata aatgagttag ctaatgctat aaaaccagtt gctataccag
     8281 gtgaagatat ggttgttcaa gtggttaaag aaggaaaaga ggctcaacaa ggtgttgcat
     8341 atctagatga tggaactatg atagttgttg atggaggaag aaaatatatg aatgagacta
     8401 taaaagtact cgttacttct gttcttcaaa ctcctgcagg tagaatgata tttgcaaaac
     8461 ctaaaaatta atatgttcaa agaaacctat aatttgggct gtttatttta aatagatggt
     8521 ccaaatttat ttatttataa cgataatgtt taatgtattc ttaaagatgg ggatttaaat
     8581 cagaaagcag ggagaattta tgagaaaaaa gattatatct ttcttagcaa catttatcat
     8641 aatattaaca tcagcaagtc agtatagttt tgcagatgat atatcaacac gtggaaaagt
     8701 aatatttata gatatgaata gaactagtat gagtaatatg ttaagaataa aatcattaag
     8761 agaagaacta gacaatagag ggtatattgg cttgatgaat ataagaggcg ataaaggaag
     8821 tgatgataga aggtcatatg ctagtatggg tgctggtggt agagctaatg tggctaatga
     8881 agaagatatt aattttgaaa gctcgagtaa ggatagaaat atagtttttg aatcagctac
     8941 aggaaaaagt gcaaaaggta taaataattt aactataaat aagtctataa atgaaaattt
     9001 aaactttgga gagtatggtt ctgtattagg gtcattagga caaagtttat ctgataatgg
     9061 attaaaagca tctgtattgg gtaattcaga tataattgaa aatggtcaac ttataaaaaa
     9121 tagaaactta tgtctaactg caatggatga atatggaaga atacctaatg gaaatgtaga
     9181 tactataaat aaaaaagact tatctatgcc ttatggaata tcaactgatt atgataaatt
     9241 gattgtagag actaaagaaa cttacaaaaa taatgatgtg atttttgtgg agcttggaga
     9301 cacatataga ttagatttat ataaaccaaa tttaaatgaa aaaacatatg agagtatgaa
     9361 agataacata gaaaaaaata tagatatata tttaaaaaat atattttcta tggttgaaga
     9421 aaatgacact atctacatag caagtgcatt tccaagtgat ttagattata aaaataaaag
     9481 aagactttct ccaataataa aattaaatgg agaagggaaa ggtatattat catcttcaac
     9541 tactagaaga gaaggtattg taacaaattt agatgttggg gttgaaatat tagacaactt
     9601 taatataaaa aatcaaaata tggttggaag aaaatatgaa cttattaaca gagatgataa
     9661 taaagaattt ttgatggatg aatatcaaaa gatagtatct atttcaagca taaggtctac
     9721 aatactgaat ggttttgtaa gtatagtttt cttgtcatgg atagttgcta tgattgcaat
     9781 tttatttaga aagcatatat caaaaaatta taaagaaaca acatttttta tattaaagga
     9841 acttttaaaa ataggaatag taatgccact gtcatttatg ataacaccaa taatgaattt
     9901 caaaacacca ttagcaatat ctttaggaat aattataata acacttacta tatatcttat
     9961 aagtaaagtt ttaataaaga acgatttaaa aaacatgctg ttttttactg gattaaccat
    10021 agtaattatg gttattgatg ctgggtttgg ctcatattta atgaaaagca atgttatgag
    10081 ttatgattgt ataattggag ctagatatta tggagtagga aatgagtatc aaggtgtggc
    10141 aatcggtagt gcaatattta catttgcaat tttacttaca tataaaaata taccaaagtg
    10201 gtatataata gttttttctt tggttatctt aattacaagt gcatctccaa ttatgggagc
    10261 aaatgttgga agtgctattt ctgaatgtgt tgcattttta ctatttatac tattgatata
    10321 taatgtaaag atagatttca aaaaaatcgt attattaggt atagcagtag tatttgtatt
    10381 aggagttttt gtagcaatag atatgatttt gggaagtaat tcacatttag gtatgtttgt
    10441 aaaagaaatt tactttaatg gaccaggaga aataatacaa acatttagta gaaagataga
    10501 gatgaattta aagctagctc aaaccagtgc atgggtaaat atactgttaa ctggaatagg
    10561 tgtaatttta gttcttatga ttaatcagat tagatacttt aagcaactga tggatgagta
    10621 tccaattgta tttaaaggat ttatagcatc aatagcagga tgtttagtaa ctcttttagt
    10681 gaatgactct gggattgtgt catcagctac agcctttatt tatgtaattg tacctatgat
    10741 aacattaagt ataaatctta cagcattaaa agaataagta ctgtatgaat aatatgtaaa
    10801 tattaataca ttaatttaac ttctttattt acaaaataaa gaagaaagtt tataaaaaat
    10861 aaattataat aaaaaagagt ttatctcaaa attgattatt ctaatcaaat tttaagataa
    10921 acttttttta ttataaagat aaataacata taacctcata ttttaggtag atttttttct
    10981 tatatgttta ggagtgcatt taaatttttc cttaaaaatt ctgtaaaata attttgtatt
    11041 agtaaatcca tgagcttcta atatactgca taaggtatca tttgtgttaa ttaaatcttt
    11101 ataaatatga gaaatacgca catcatttaa atactctaag taagtttgtc ccatatactg
    11161 tttaaatttt ctacaaaaat attctggttg taagtgtgct accttagcga ttttgttaat
    11221 agaaataggc attttgtaat taatctttgt atattctaaa actggttcaa attttgacaa
    11281 atcaagtact gtttttttca tatttttatt acctacagat attttaaagt ttgtatataa
    11341 ttcaaataaa agttcaaata ataagcttgt aaagagcaga ttagctgcag gaggagaact
    11401 aagtttaatt tctctcatgt tttctaatat aagtttaact ttacttgaat tttttttata
    11461 gttattgtca ttggcattaa ccttaaagtc aaaatagcaa tttttaaaat cagggatata
    11521 tttgtttaga aaagacaatg gtacttgcaa taatatagag gtattttgat aaatacatct
    11581 agttgagtga ataattccag aattaatgaa catacactca ttagctttta aattataagt
    11641 gtaaccctcc atgttaattt gtaaatcacc agaagttata taaattattt ctaatgagtt
    11701 atgccaatga tttgctacat aactaccact atcatcaaat agtttgaaat taacatctaa
    11761 attatcaata ggttcaatta tttcatgtga aaaattattt ctgttcataa taatatcctc
    11821 cttataattt taaattataa atagtaaatt tataatgttc aatattaaaa agataatata
    11881 gactaataaa ttagataata aacatactta agaaaacgga taaaatcaaa tataataact
    11941 gtatattaaa ataacttaaa aaaattattt tattataagt gcaaaatata cctatttaaa
    12001 taagataaga ggggaggatt ttatgtgtat ataaaaggtg taaatttagg aggttggtta
    12061 gttttagaaa aatggatgac ctcaagttta tttgaaggca cagaagctga agatgaatac
    12121 tatttaccaa gacagctttc aagagaggca tatgaatcta gaattaagac tcatcgtagt
    12181 gaatacatca cagagagaga ttttgcaacg attaaatcta tgggatttaa ttcagttcgt
    12241 ataccagtac catactttat ttttggagat tgtgaaccat tcataggatg tgttaaagaa
    12301 cttgataaag catttgcttg ggcagataag tatgggctta gtatattgat agacttacat
    12361 acagttccag gaagccaaaa tggatttgat aatggaggaa tatcaggtat atgtagctgg
    12421 tcacaaaatc cagaatatgt agcgtttaca ttaaatgtat tagaaagact tgcaaaacgt
    12481 tatgggatga ggcatgaatt gtatggaatc caaattttaa atgagcctat aacagaaaga
    12541 atgtggaaca taatgaatgt tccaaatcgg tttaaggctg tagataagga gatggcaagg
    12601 gggagtaaac ctaattcatt agagttttta cgtgattttt atataaaagc atatagggta
    12661 atgcgtcctt atatgagaga ggaaaacgtt atagtgtttc atgatgcatt tgaattaaaa
    12721 gcatggaaag actttatgag agaggaagaa tttaaaaatg ttgttctaga tacacaccaa
    12781 tatttgatgt tagctgaagc agatggatgt gaacaaagta ttgatagcta tctcaaatat
    12841 attagagaaa attatgctaa agatatttta caaatgcaaa aatattttcc tgttatatgt
    12901 ggggagtgga gcttatttaa ttcatatgtt tgtggtatag atacaaatgg aggtcaatca
    12961 cctctaaatg ggattgaatc aaatatagat aaattatcaa aagacgataa aagagaatta
    13021 tataggaaga tagcaaaagc acagcttgat gcatggagaa atggaagtgg tcactactat
    13081 tggaattata agttattact agatacagtt aatgaagaag gttggatagg atgggatagt
    13141 tgggacctag gcaaatgtgt agctcaagaa tggtatccta tagaatatta aactaaaatt
    13201 ggaggaaaag atatgattaa aaaccctata cttccaggtt ttaatccaga tccttgtatt
    13261 tgtagaaagg gagatgatta ttatcttgta gtttcatctt ttgaatggtt tccaggtata
    13321 cctgtttatc actctaaaga cttaaaaaat tgggagttat atactcatat tttgacagat
    13381 gaaacaaaaa tagatttgaa gaagcttcct tcatctaaag gaatttgggc accctgtttg
    13441 acttattgtg aagaagaaga tttattttac atagtttatg gaattatgaa ctctatgaat
    13501 gcaagatatt ttgatgttga taattattta attacatcaa aagatataaa gggagagtgg
    13561 agtgaaccag tatatctaca ctcatcaggt tttgatgcgt ctatttttca tgatgatgat
    13621 ggtaaaaagt ggattgcatc attagactgg gagactagag aaggatatga aaaacctggt
    13681 gttatatgtt tagtggaata ctgtacaaaa aagaaagaaa tagtaggata tccaaagaga
    13741 atatggtcag ggggaactga tagaggttgt attgaagcac cacatataac aaagcgtgga
    13801 gattactatt atataatgtg tgcagaaggt ggaactggat atggtcatag tgtaactatg
    13861 ggaagggcta aaaatatatg gggaccttat gaaaaagatt caatgaatcc aattgtaact
    13921 tcaattccag gcgattttta tgaaagacat gaccctgacc atctaaagcc taaatactat
    13981 aatccagaat caaaacttca aaaatctggt catggaagct atatagaaac aacatcaggt
    14041 gaagtatatt tagttcatct aacatctaga ccatttgtac cagagttaag gtgtacttta
    14101 ggtagagaaa cagctattca aaaaatgaaa tggacaaagg ataattggct tcgaatggaa
    14161 gatgaaagta atttggcaaa agaatacgta tcagaaagta aactagaaga acatttagtt
    14221 tcatctatac caagttttga tgactttgat tcaaatgaat taggtttaca gtactatgct
    14281 cctaggattt caccattatc atttgcagat gttaagtcta gaccaggata tgtaaggatt
    14341 agaggacaag aatcaagaac atcattaaat aaagtaagca tattggcaag aaaattaact
    14401 tcagtatatg caagaataac cactaagatg gagttttacc cagaggtaca tcagcatagt
    14461 gcgggtttga taatgtatta tgataatatg aattatataa atttaagaaa gtattatagt
    14521 gaaacattgg gtcaaagtgc actatcaata atacatcttg aaaatggaga gaaaacggaa
    14581 tttttaaata ctaggatacc aataaaagat ataccaatat atcttcgtct gtatatacaa
    14641 ggtcgtaagt catattttga atggagttat gatgaaaaaa actaccagag aataggaaag
    14701 gtatttgata caactaagtt ttcagatgag tactgtaaat atggagaatt tacaggtacg
    14761 tttataggat taacttgtgc agatcgtgta aagcataaac actatgctga ttttgatttt
    14821 tttgaatata tagtagatga atcaaaagat gtcgattaat aaaatcatta ctaaaatttt
    14881 cagagcaaag gtaaatctta tgctctgaaa taagaaacat tagaaggcaa atcttacaaa
    14941 aaggagtatg gtattatgaa tacagaagtt aatgttaaag caactacgat tagatatgaa
    15001 aagatttcat tttttgaaag aatttcttat ggttgtgggg atttggggtg caatatcata
    15061 tactcagcta tgtcagcatt tttgctgttt tattatacta attatgcaga tgttagtgca
    15121 gcagctgtag gtagtattat gcttgtatct aggatactgg atgggtttag tgacttaaca
    15181 atgggaatta tagtggatag aactaaatcc aaatacggta aggctaggcc ttggattttg
    15241 agaatggcga ttccttttgc tatagcagca gtattattat tttctgttcc atcaaatctt
    15301 ggtatcactt ctaaattaat ttatattttt attacatata acttagtttc tactgttatt
    15361 tatacagcaa tcaatgtacc ctatgcaact ctaaattcac ttataactca aaaccagtat
    15421 gaaagaggag tattaagtat atttagaatg atattagcca cttgtggtac cttactaata
    15481 aatggactta cactacctct agttgaatac tttggaaata atttatcagc atggactaaa
    15541 acattctttg tatttggaat agcatctata atggtatttt ttataacatt tactggaacc
    15601 aaggaaagag taaaagcagt taagcaagat aggaatgaag taataccatt taaaataggt
    15661 attaaatctt tatttagaaa taagtactgg atacaaataa cattatgttt agtttgcata
    15721 tttattgtat ttgcgataaa tggtggttca tccgtatatt atgcaaagtt tattttggga
    15781 gatgaaaaat tatttggtcc tataaatatg gtttcaaata tatcccaaat tatagctatg
    15841 tttatggttg ctccttttat aaaaaaattt ggaaaaagaa atgtattaat agtaggttca
    15901 ataatattaa tatcgtcaaa tgttatgttt ataatagttg gacaaaatca tataggagta
    15961 atatttgcaa gtgctataaa gggaatagga agtgctggaa tagcatcaac aatgtttgct
    16021 attgtttcag atactattga atatggagaa tggaagactg gatatagaac tgaggggctt
    16081 ataaatagtg catctagttt tgggtttaaa gtgggaaatg gattaggttc agcaatatta
    16141 ggtgcagtat tatcaattgg tggttatgta ggaacatcag ctactcaaag tgatttagct
    16201 atagtatcaa taaaagtatg ttttatatac ctacctatat ttataacaat attacaaatt
    16261 atcataatgt ctttttataa gcttgataaa gaatatagta ctatacttaa tgaattaaat
    16321 actaaataat gaaaaagttt cttataaagt cttaagttta attaaggtct attgtaaatg
    16381 ttccctataa aaactattat atatttatga ttattgaaga atttttagag aagcgaataa
    16441 taaaaatata agaagacttt aaatgatgaa atggttggtt ttttagaaat gatttaataa
    16501 gttattgtta tttaaagcat tataagtgga caattaattt ttaatttatg gagatgatat
    16561 ttaacaatta gaagtaatat ataatatgca ctaagaagca atttttcaac tatttatgaa
    16621 ggtggtggta caagaaaagt atcacctttt ttttgttata caaaaaaaca gttgactttt
    16681 tatatgaacc ccttctataa tgttactgaa attaagtaaa atgattgaac aaatgtaaag
    16741 ataattgaaa agcttgattt tggaggaaag caaatgaaaa aaattggaga cttaagatta
    16801 atgatgaagt gttgttcact ttattacgaa gataatttga atcaacagga aatagcaaat
    16861 caattgggaa tatcaagacc aacaatatca agaatattaa aagaagcttt tgaacaagga
    16921 atagtaaaaa tacagatagt tgatgttttg aaaaatgatt accaaaaaat agagagaagt
    16981 ttggagagaa aatataaatt aaaagaagta atagtagtag atgataaaca ggatgcacta
    17041 acacaaaaac aagaactagc aagagctgta tctgaatatt taactagagt agtaaaggaa
    17101 aatgatataa taggtgtttc aataggaaca acactaaaag aaataccaag atatgtggaa
    17161 aaaagtaact gtaaaaatgt tacatttata cctctattag gagggattgg agacaatgaa
    17221 atagatattc atgcaaatca aatagccgta agtttagcga gggcttttgg aggtgatttt
    17281 aaattattac atgctccagc tgttatgtct gacttgtcta caaaagagaa actttgcaaa
    17341 gacgaaaaaa ttaaagaagt actagattta atagataaaa caactatagc aattgtgggt
    17401 ataggaaatc ctatgagttt aaactcaaca ataatggctt ctggatacat gaatgaagca
    17461 gatatagaag atttaaaaaa atacaactca ataggtgcaa tttgtttaca agcctttgat
    17521 aaagaaggaa aaacttctat acttgaattt aatcaaagag tattaggagt gaaactggaa
    17581 gatttaaaaa agataaaaag aacaataggt gtcgcttcag gagatgaaaa aatagaggca
    17641 ataaaggcat cattaaaagc gaaatttata aatagtttag caattaatca cagtttagca
    17701 cttaaattat tagaagattg tgactaatag aaagatagat aaaagttaac aggagggaat
    17761 gtatatgtca atatcaaaag aaacattgct tgaaatgtat aagagaatga atcaggctag
    17821 aaaatttgaa gagaaagtta gttggttttt tgcaagaggt atggttcatg gaacaactca
    17881 cttatctgta ggtcaagaag cgtcaagtgt ggcggctgta atggctttag aaaaaggaga
    17941 tttagtatca ttaactcata gaggacatag tcaatttata ggaatgggaa tagacctaaa
    18001 taaaatgatg gctgaactta tgggtaaaga aactggattt tgtaagggaa aaggaggctc
    18061 aatgcatata gcagatattg agtcaggtaa cttaggagca aatggagttg taggtggagg
    18121 tcttacaata gctccaggag ctgcacttac acaacagtat aaaaaaacag gcaaaatagt
    18181 tttatgtagt tttggtgatg gagcatcaaa tgaaggtact tttcatgaag gaataaattt
    18241 atcttcaata tggaaattgc caataatatt ttactgtgag aataatttat acggtatgtc
    18301 aacaagcata aaacgtcata tgaatataga aagtattgcc actagagcag cttcttatgg
    18361 aatagaagga atatcaatag atggatataa cccaatagaa gtatacgaaa cagtacaaaa
    18421 agctgctgag aagtgtagaa gaggagaagg acctgtatta attgaaagta gaacttatag
    18481 atggttaggt cattcaaaat cagatgcaaa tgtatataga acaaaagaag aaatagaatc
    18541 atggaaagca aaagacccta ttgaattttt aaaaaattat cttatagaaa ataatctatc
    18601 taatgaagat gaattagata agattcaaga atttgcaaag caatcaatag aagatgcagt
    18661 agagtttgct caaaatagcc caaatccaaa aatagagtct ttattagagg atgtatatgc
    18721 agactaagaa tggtaaagga ggaaaataaa aatgagtaca agggaattaa catatgcaca
    18781 agcaataaag gaagcaatgt cagaagaaat gagaagagat gaaaatgtaa tatttatggg
    18841 agaagatata ggaatatatg gaggagcatt tggtgtatca gttggtatga tagatgaatt
    18901 tggaccagaa agagtaaggg atactcctat atcagaagca gctatagcag gagcggcagc
    18961 aggtgcagca gcaactggct taagacctat tatggaagta atgtttatgg actttgtaac
    19021 aatttctatg gatgcaattg taaatcaagc ggcaaaaatg agatatatgt ttggaggaaa
    19081 agcacaagtt ccaatggttg ttagatgccc aggaggttct ggaactggtt cagcagagca
    19141 acattcacaa agtttagaag cttggttttg tcatgtacca ggagtaaaag tagttgcacc
    19201 ttctacacca gcagatgcta agggattgtt gaaagcagct ataagagata ataatccagt
    19261 catatttgtt gagaataaat tgttatacag aaaaaaaggt tttgtgccag aagatgatta
    19321 tgtaattgaa attggaaagg cagatattaa aagagagggt actgatgtta cagtaattac
    19381 ttatggaaga atgttacaaa gtgttgaaga agcagcagag actctatcaa aagaaaatat
    19441 aaatgttgaa attatagatt taagaacttt atatccatta gataaagaga ctatagtaaa
    19501 gagtgtatgt aaaactggga gagttttaat ttgccatgaa gcagctaaga ctggtgggct
    19561 aggtggagaa atatcagccc tgataacaga aagtgaatca tttgattatt tagatgctcc
    19621 agtaaaaaga atttgtggaa aagatgtgcc aataccatat aatccagagt tagaaaaagc
    19681 agttgtccca agagttgatg aaatagaaga agcaataaaa tctttaattg ttagataagg
    19741 agggatagat atggtggcaa ataaaataaa agccacacca gcagctagaa gtcaggcaag
    19801 aaaagacaat ataaaattag ataggctgat tggaagtgga gaaaatggaa ggattcattt
    19861 agtagatgtg ttaaattatt taaaagataa taaagcaaat actactcctt tagctagaag
    19921 aatagcagaa gatttaaata tagatttaga aactattgta ggtactggat ataatggaaa
    19981 gataagaaag tgtgatgtgg aaaaattaac tggaaaagaa actattgtat caactaatac
    20041 atctaaatct agtgagaaaa aagagctgaa aattgaaaat gaaaattcaa gaatgtttaa
    20101 cacagtagaa ggaatatttg aaaagccaaa tccaatgaga gctactgtag caaaacgaat
    20161 gtcagaaagt tattttagtg ctcctgtatt tacattcaat atagaagtag atgctacaga
    20221 attgaaggta cttagagcaa agttaatcga cacagtaaaa gaatcaactg gagtaaaatt
    20281 aactatgaca gatttaatag taatggcagt atctaaaata cttccaaatc atcaagcctt
    20341 aaattcagca tggacagatg agggtatatt tagatataaa gatgtaaata tagctattgc
    20401 agttggttta gatgaaggct tatatgttcc ggtagtaaaa aatgcaaaca aaaagtcttt
    20461 aaaagaaata gcaaaagaaa gcaaagaact tgcggaaaaa gtaaagacag ggaaacttat
    20521 gccagcagac caagaaggta atacatttac aataagtaat gttggaatgt atggtataac
    20581 tacatttaca cctataataa atatgccttc aagtgcaatc ttaggggtag gtgctacaca
    20641 agataagttt gttccagtaa atggagaggc taaaataaaa cctataatga acttatcatt
    20701 aacatcagac catagagtaa tagatggaac agtagctgca aaattcttaa aagatttaaa
    20761 agaattgtta gaaaatcctt tatcaatgct tgtataggtt ttgatatttt gttactgata
    20821 taaatagttt gaatagatta tattattata attgtttcag taaatttaaa gcttttatat
    20881 attaagaatt atttttaaca gtaactatat gtgaatatga tttgcttaat taaaatatat
    20941 gaaaataaat aggaggagag cctatgtcag tagaagtaat aatgccaaaa gcaggagtag
    21001 ccatggaaga aggtactata gtatcttggc taaagcaaga aggcgaagaa gtaaagattg
    21061 gagaacctat actagaaata acaacagata aagttaatat ggagatagag tcagaaggag
    21121 aaggtacact tgctgtaatc atacataaag aggaaggtga agtattacca gtatttacag
    21181 taataggtgt aatagcagaa aaaggggaaa atcaagagga agtaaaagct aaatatttat
    21241 ctggaaatgt ttcaaaagaa gatatagttg aagagaatca aaatatagaa tttaaagaag
    21301 aaaaaataaa caaaaaagag tgtaatcatg attatgatgt agtggtaata ggaggaggac
    21361 ctgggggata tttatcagca ttaaaagcag ctcttttagg tggaagagtt gctttagttg
    21421 aagaaaatat actaggaggt acttgcttaa atagaggttg tatccctaca aagacttata
    21481 taaaaacagc agaaatatta gaagaaattg accaattatc aaagagaggt gtaaaagtaa
    21541 ctgtagataa agaacaggac attaagaagg ccataaaata taaaaataga gtagttaaaa
    21601 aacttacagc tggtgttggc ggattattaa aaagtagaga tgtagatgtg tttaatctta
    21661 aagctagtgt taaagaagag cataaagtaa ttttatcaga tggaaaagtt ttagatacag
    21721 aaaatataat aattgctaca ggttccaaag taaggatatt acctataaaa ggaatagaat
    21781 caaatttaat aataactagt acagaagcat tagatttgga aacagtgcca gaagaattag
    21841 taataattgg tggtggagtg attggttgtg aatttgcaga gatattcaat tcaagaggtt
    21901 ctaaagtaac tatagtagaa atggaagata gagtaatccc aagaatggat aaagagttaa
    21961 gcgaatcttt aaaatattct cttagtaaaa aaggtataaa tgtattaact aaaaagaaag
    22021 tttctgaatt taaagaagaa ggaaataaca tattagtgtg cattgaaggt gaagagccaa
    22081 taaaagcaga tttatgtcta tatgctatag gaagagaagc taatctttct ggcatagagg
    22141 acttagatat taaaatagat aaaggttcaa tagttgtaaa tagtaaaatg gaaacaagta
    22201 taccaagcat atatgcagta ggtgatgtta ctggaggtgt aatgttagct catgctgctt
    22261 ttaaaatggg agaggtagca gcttcaaatg ctctaggtgt gaataaagaa gttgacttag
    22321 gtgcattacc tagttgtgtt tatacaattc cagaagtagc atcagttggt attacagaag
    22381 aggatgctag aaaaaaatat aatgtaaaag ttggtaagtt taattttgct ggaaatggaa
    22441 gagctttagc ttctggtcaa gaacaaggat atgtgaaggt agtagcagat gctaaatatg
    22501 gagaaatttt aggtatacat atgtttggat gtggtgtagc agaacttata aatcatgcag
    22561 catcatttaa agcgttagag atacctactg atgaagcaag tgagttgata tttggtcatc
    22621 catgtacttc agaagcttta atggaagctt tggcagatgt aaatggagaa tgtttacact
    22681 tacctaaaaa ataaatctaa acataaatta ttaatttaag tctattatta atattaattt
    22741 atatcataaa attttaaaac acttacttag ccatacccct tattatgcaa ataatttatt
    22801 tgcataataa ggggtatttt ttatggaaaa atatagaata tagaatcctt gtagtttttg
    22861 ttgtacaaaa aagatagtaa tataaaataa cattatcatt ttagaatgat ttcaattgta
    22921 attaggaaaa gtgatttttt tgtttttaaa aaaagtatta tttgtaaaat ataaaaaaaa
    22981 atttcttaat atttgaataa aagaatagct taatttaaat gttattactt tataaaaaaa
    23041 taataagtac aaaaaaaaca atatatggca tataagttgc tatataaata ataaggaggg
    23101 aacaattata aatgaggcaa agaataaaaa acttaattga aaatgaagat aagaaaaatc
    23161 ctttaacaga tgaagcgatt tcaagtaggt taaatattcg aagagaagat gttacctttt
    23221 tgagaaatga gttaaaaata gaagattcaa gacaaagaag aaaagttgtt ttaattaaag
    23281 ctataaaaga tattttaaaa gaagaaaaaa ctataaataa aaatgatata acaagaagat
    23341 taaacagcat tgggtttaca gtatcaagat ttacagtcat acaatactta aaagagattc
    23401 aagattctgg tgacctaaaa cttgataaag agagtgatga taaaaaagaa gaaataatca
    23461 ataatagaga aaataaagat atagcatttg ataaacttat aggtagtagt ggtagcttaa
    23521 gtacaattat aaaactagcc aaagcagcaa ttctttatcc acctcatggt cttcatacta
    23581 ttattatggg gccaactggt gttggaaaga gtgagttagc agagtgtatg tataaatttg
    23641 cagtagaatc atccagattc cctaagaatg caccatttat agtttttaat gcagcagatt
    23701 attcagagaa tccacagctt ttgctatcac agttatttgg acacgttaaa ggtgctttta
    23761 caggagctga tgagaacaaa gaaggattag tatcaaaggc agatggagga atattgttta
    23821 tagatgaaat acatagatta cctcatgaag gtcaagaaat actatttcaa ctgattgata
    23881 aaggtatggt cagaaagtta ggagaaacaa gactaacaca taaaatagat gtaatgatta
    23941 tttcagcaac ttctgagcca atagattctc atttacttaa tacatttaaa agacgtatac
    24001 ctgtaacaat tgaaatacca gaattagttg caagaccatt aaatgagagg tttgacatta
    24061 taaataattt cttcttaata gaagctcaaa gaatgggagc aaacatacat ataaagtcag
    24121 atgtattaaa agctcttatg ctttacgatt gtataggtaa tgtaggtcaa cttagaagtg
    24181 atattcaagt agcttgtgct aggggactat taaaccaact tacaaacaaa ctaaaagaag
    24241 taaatataag catatcagat gtacctggat atgtaaaaca aggtcttata aaaatcagaa
    24301 attgtagagg gaaaatagaa aattatgtag atggggattt aattatagac tcaaatcttc
    24361 aaggtgagat agaaaagaaa aatgaagata tatatacttt tccagatgaa atatacaaac
    24421 ttactgaaag aagacatatt gatttattgg aacaaggttt agattttgat gttataaata
    24481 gaatcattgg aggagaatta gaaagttgca ttcaaaaata cataaaacaa gttaaaaaag
    24541 tttctattag taacaatctt ccagatataa gtccaattgt tggaaaatat atcgtagagc
    24601 taacttatga agtaatcaaa atagcatcac aatacctaag ttcactagaa ccgaatctac
    24661 atttatgttt agcagttcac ctaaaagcag cttttgagag attaaaagaa ggaaaagtaa
    24721 tatcaaatgc tcatttgaaa gaaataatag gaaattatcc agtagaatat tctgtagcag
    24781 aaaaggtagc tcaatattta aaggaaagat ataacataaa cttaccaaaa gaagaaatag
    24841 gtataatagc aatatatttt aaaatgagta gtagaagaga tatgtctgaa caaaaaaaga
    24901 taggcgtact tcttatggct cacggaagag tagcatcttc tgtatgtgag gttacaaata
    24961 aactattagg aattaatcac gcaaggtatt tagatatgcc atttgagaag aagccggaag
    25021 aaatgattga agaagctact acaataataa aagatattga tgaaggtaga ggtgtgatta
    25081 tattagtaga tatgggttca ctttcatatt ttggagatat tatttctgga aagactggaa
    25141 ttaatataag gacaatatct agatttgata cattgctttc tatagaggtt atacgaagag
    25201 cagttttacc agatttaaca atagacgaga tagttgatga agttaaaggt atagagccac
    25261 aagtaaaaga aaaggttagt aaatgtaaca atacaaaagt agattttaga aaacctataa
    25321 ttttaacatt atgtattact ggattagggg gagcttgtaa gataaaaaaa atcttagagg
    25381 aaaatattaa aagtataact gaaaaggttg atatagaacc tataggtatg attgaatatc
    25441 caagtcttga tttagagata aaacaaataa gcaatgataa aaatgtaatc gccatagttg
    25501 gaacaattga ccctcagata gcaaatatcc ctttttttag tctagtagac ataaatgata
    25561 aagcgaagtt aaatcaaata aataacctaa ttgatacaaa tcaaattggt gaaacaaata
    25621 aaaaatatga attatcagat ttaatacata aagaagatat atttgtggat ttacaggtta
    25681 aatcaaaaga agaagttcta aaatatatga gtaaaaaact tagagagaaa ggttatgtga
    25741 caaaaaattt ctataaaaaa gtagtggaaa gagataactt atttccaact gaattatcag
    25801 atgaaatagc aatacctcac acagatggaa tagatgtaat taggccagct ataagtatag
    25861 ccatcttaaa gaagcctatt gtgtggagta aaagtaaagt aaaaataata cttttattag
    25921 ctgtagacca aaagtgtttt aatccattaa gcactctact ctcatttata gaaacagatg
    25981 aatttaagaa gataaaaaac attaaagata gagattttat tagggagatg atattagatg
    26041 gagtcaatga aaataattag tagcaattta atttttaaaa atattgaagt gtctaacaat
    26101 gaagatgcat taaagttttt gggacaacgt ttgtttgacg aacagtatgt aaaagaaagt
    26161 tacatacaag ctgttgtagc aagagaaaaa aagtatgcta cagggttacc aacagaaata
    26221 tacggagtgg caataccaca tacagatata gtacatgtaa atgagccagg aatagcaatt
    26281 ggaatactga ataaacctgt aaaatttata atgatgggta cagacgatac tgaaattgat
    26341 gtaaaagttg tatttatgtt agcagtgaaa gagccacaag aacaattgca actattagaa
    26401 aaacttatga ctatatttca agataagaat atactaaata atattgttga cttaagtgaa
    26461 gaaagtgtga gtgatttatt aaactctaaa ttgaaaaatt aaacatgtaa agtatattaa
    26521 aaatgtaaaa ttaaacatgc aaagtatgtt aaaaaatata aaaagattat taaagatttc
    26581 taactataga tatctttaaa ataaaataat ttcaatgggg gtgactaatg tggctaaaaa
    26641 gatattagtt gcatgtggaa caggagtatg tacttcaaca attgctataa acaaattaaa
    26701 gaaagcactt caagacattg gtaaattgga tatggttaat attacacaat gtaaagtagt
    26761 agaagttgca tcaaaagcac ctgattatga tttaataatt tgtactacac aagtatctag
    26821 tagtataaaa acaccagtga taaatggatt acctttttta actggtgtcg gaatggataa
    26881 attaatcaat gatgtattag aagagttaga attataatta aaaaaattaa ggaacatggt
    26941 ctctaaaatc ataagtttta gagacttaaa attaaataat ggagggtctt tatatggaga
    27001 tattaaattt cattgttggt ctaggtgcac aagtaatgat gccaataata atatgtattt
    27061 ttggtttgat attaggtaca aaactaggaa aatctttaag agctggttta actgtgggag
    27121 ttgggtttat aggtcttaac actattatag ctttgctaac agataattta ggtccagcta
    27181 cacaacaaat ggttaagaat ttagggctaa gtttatcaat aatagatgta ggatggccag
    27241 cagcatcagc tatagctttt gcatcaacag taggggcttt aattattcca ataggtcttg
    27301 tggtaaatat agttatgcta attactaata ctactcaaac tgttgatgta gacatatggg
    27361 attactggca ttttgctttt acaggagctt tagtggcagg tgctactcaa agtgtaatgt
    27421 ggggagtatt tgcatcagta gcaaatatgg taattgtatt ggtaatggca gatttaactg
    27481 ctccaggtat agaggaatat ttaggtatgc caggtatatc tcttcctcat ggatttacac
    27541 aggcatttgt tccaattgct atagtagtaa ataaattatt agacctaata ccaggtataa
    27601 ataagattga gataaatgct gatacattgc agaaaaaatt tggattattt ggagaacctt
    27661 taataatggg gtctgtaata ggtgtaataa ttggtattgc tgcgaaatat gatataaaag
    27721 gaatattaca attaggtgta actatgggag ctgtattaat attaattcca aagatggcag
    27781 cattattaat ggaaggatta ttaccagtat cagaagctgc acaggaattt attgagaaga
    27841 gatttaaaaa cagaggcaag atttatatag gcttagattc agcagtagga ataggtcatc
    27901 ctgttacatt atcagttgca ctagtgttgg ttcctttgac tatacttata gcagctatat
    27961 tgccaggaaa taaagtttta ccatttgcag atttagcagt aattccattt gcacttgtat
    28021 taatcgtacc aattacaaag ggaaatgtgt ttagaacatt aataatagga ataattataa
    28081 ttacttctgg attattaatc gcaactaact tagctccatt atttacccaa atggctttaa
    28141 atgctagttt taaaatgcct gaaggagcaa ctatgatttc aagtatttgt gatggtgcga
    28201 atccattaag ttgggtattt gtaaaagtta tgaactataa agttattgga ggagtagttt
    28261 ttggagttat agctttagga atggctattt acaataggaa tagaataata aatgaaaata
    28321 aaaagctatc tatagaagaa taaggttaaa acaattttat taaggagtgt ataggttatg
    28381 aaaaaatata aatatgattc taaaagatac ttactcagtg ttacaatacc aggatttttt
    28441 atgattttaa tattgattta tgcactgttt aataactata caaattttaa tttgaatata
    28501 tatagtttgt taataattgt ctgtacttat ggtatattca atacttttat atcatcaagt
    28561 aatcctaaaa aaattattat tgatagcaaa tgtatacatt ttacctcatt tatgactaca
    28621 cataagtatg aaattaaaga tatagaaaag ataagaataa gagaatttta taataaaaaa
    28681 atttatgtaa aaattaatga tggaaacctt tttaaaggta ggtattggat aagaacaaat
    28741 atgtttaata atagtatagc attatatagc tatttcattg agttggaaga gtgcttatat
    28801 ccagattcac taaaatttag aaataaaaaa tttgagaata agaacatata aaaataaaaa
    28861 attgaattga agcctagctt aatctttttt gataagatat aagtttgaac tttatactaa
    28921 atttttagaa ttttcaatca agacaataac ataatagcta agcaattaga acacaattaa
    28981 gatgtataag gcatgtatat gatgaatggg aggatttaat taatgttatt agagaaatta
    29041 agaaaagaag tattacaagc atctttagac cttttaaact ataatttagt gacattaaca
    29101 ggaggaaatg taagtgggag agatgaacaa actggctata tagcaatcac cccaagtgga
    29161 atggattata gaaatcttac accaagtgat attgttatag tagatgtaga tggaaatatt
    29221 atagatggaa aatggaaagc atcagtggat ttatcagacc acttgtatat atataagcat
    29281 agagaagaca taaatagtat aattcataca cattctacat actcaagttg ttttgctatt
    29341 ttaaatgaac ctatagaatg tgcaagtaca actcttgcaa atgaagtagg gggttcagta
    29401 ccagttgcaa aattttcacc accaacatct aaaaagatgg gaaaatgtgt tattgaagca
    29461 attggtgaca aaagagcatg tcttttagca aatcatggcg taattgcagt tggtccatct
    29521 gtaggacatg ctcttacagc cgctgtaatg ttagaagatt cagcaaaagt atattactta
    29581 gcaaaatcaa ttggcacacc agtgctactg cctgatgaag aaattcaaag agcgagagat
    29641 gtatttttta atgtctatgg tcaggataaa taaattagat aaaattttaa aagtaatatg
    29701 aaaatataga tattcaatga tattaaggag atttataatg atataataag tttaatagtt
    29761 aagaagtaaa atatgaaatg taatagtata taaattcatt taagactaaa aagaataaga
    29821 taaaataaaa aggagtctgt atttctatgt attacttatt aaacaaaagc acaaatcctt
    29881 actttaattt ggcattagaa gaatatctat ttttaaatga taaatataat gatgatatta
    29941 taatcatatg gagaaatgaa gaatccattt ttataggtaa aaatcaaaat ccatatcagg
    30001 aagtatatca tgatgttatt gaaaagggag aaattcctat tttaagaaga atatctggag
    30061 gaggaactgt ttatcatgac ttaggtaata tcaatatgtc attcatacaa aaagataggc
    30121 aacttcatga gatagatttt ttggagcata ctaagtttat gcaaaagatg ctttcaacct
    30181 taggactaga tgtatctata actgaaagaa aagatttatt tttaaatggg aaaaaaattt
    30241 caggctctgc acagtctata aaaagaaaaa attctcttta tcatggaact cttctttacg
    30301 attcagattt aaataaatta actaaatatt taaattcaaa taaagcaact gaatcaaatg
    30361 caactaaatc tgtttcaagt aaagttacaa atattaagtt tttactcgaa aaagatataa
    30421 attacttttt agattattgt gttgaatatt taaaatctaa tataaagaat ataaaagaac
    30481 ttgaattgga tgaagaagat attaaaagtg tatacaatat aggagaagat aaatataaaa
    30541 agatagagtg gacatatggt aaaacgccga aatttcaagt agtgctacca tttaataatg
    30601 aaagtaaaat aaatatacat gttaacagat ggaaagtatc aaaatttttt atttcttatg
    30661 atgataatgt aattaatatg gacaagttgt tagatttgaa tttctttaaa gaagaaataa
    30721 aagagagtat tttagaaaat tatccacatt acatagattt aatcaattta atattttaat
    30781 ataaaacctc taatttgata aaattgtctt atatcaaact agaggttttt aatattcttg
    30841 catattaaca accattacaa aattgtaaaa ttaaaaacaa cactattgaa aaatcaatat
    30901 atattgtaat aaatatatgt gtttttcatt agcaaataga cattaactat ttatacaact
    30961 gaaaatttaa tatataatta atcagatggt taaaataaaa atattaaaag ttttaataaa
    31021 tgatggttaa aaataaaata cgaggtgata ccatgtatag tgttataatt gttgcagcgg
    31081 gaagcggaag gagaatgaac ttagatataa acaaacagtt cataaaatta agagaaaaag
    31141 aaattattgc tcatacaatt caagtttttt atgaaaatat aaatatagat gaaatagtgg
    31201 tatgtataaa aaaagaagaa gaagattttt ttaaagaaaa tataataaat aagtataact
    31261 ttaaaaatat aaaaatagca tatggcggaa aagaaagaca ggactcaata tataatggtt
    31321 taaaaaaatt agatgaaaat tgtgatatag tacttatcca tgatggagca agaccatttg
    31381 tcgaccatag aataatcaat gaatctataa aggtagctaa agaaaaaaag gctgtggttg
    31441 taggtgttcc agtaagtgat actataaaga tagtgtcaga tggaacagta caagaaactc
    31501 cagaaagaaa tttgctgtgg gcagcacaaa cccctcaaac ctttgaatat aatctcataa
    31561 tagatgctta tgaacaagca tataagaaca attattatgg tactgatgat tcaatgttgg
    31621 tagaaaatat aggtcaaagt gtcactatgg taatgggttc ttatgaaaac ataaaaataa
    31681 ctagtccaga agatttaaat atagcagaac aaatattaaa tatggaaaaa agagatgaag
    31741 ttaacagtag aaggagaatt atatgagaat aggattagga tatgatgtac acaaattaac
    31801 agaggataga aaacttataa tcggtggtgt agagatacca cacgataaag gtttgttggg
    31861 tcattctgat gctgatgtat tgatacatgc aataatggat tctattttag gtgctctagc
    31921 attaggagat atagggaagc atttcccaga tacagatgaa gaatataagg gagcagatag
    31981 tatgaaatta ttagaacatg tgtataatct aataacaagt aaaggatata aaattggtaa
    32041 tatagacagt actataattg ctcaaagccc taagatggct ccatatattg agagtatgag
    32101 aagtaatata tcaaaagttt taaacacaga cattgataat ataaacataa aggcaacaac
    32161 agaagaaggt cttggtttta caggagcaaa acagggaata gcttcacaaa gtatttgttt
    32221 attattgttg acaagtcaga ataattaata tattattagt aataagttta atggatatca
    32281 agtaataggc ctatatagaa tatctataat gtatgaattt aagtgaaatt aatataaata
    32341 taaaattagc tatgataaag aaatagtaaa atgatgaaat cttcacagag agaaaacggt
    32401 ggtgagagtt ttcaagagga agtctttgaa cctgtctttt agcttaaagg tataaaaatc
    32461 tttacggtag gaaccgttat attcccaaaa gagagctatt catatagcta actagagtgg
    32521 taccgcggaa attaaacctt tcgtctctat acttgtatag agatgagagg ttttttatat
    32581 tttcaggaag ttaatattca ggaggaatct attaatgaaa atgtcaaaaa tgtttatgcc
    32641 gacattaaaa gaaatcccag cagatgctga gattacaagc catcaattaa tggttaggtc
    32701 aggcatgata aaaaaaatga cttcaggtgt ttataaccaa ttaccaatgg ggttaagagt
    32761 gttcaaaaaa attgaacaaa taattagaga agaattaaat aaaaaagatt gtcaagaaat
    32821 actttgtgca gccttacttc cttctgaatt atggaaagag tctggaaggt ggactgctat
    32881 gggtgaagaa atgtttagac taaaagacag aacagaaaga gagtactgtc ttgggccaac
    32941 acatgaagaa gcattcactg atataataag acaagaaatt acttcatata aacaattgcc
    33001 actaaacttg taccaaatac aagtaaaata tagagacgag agaagaccaa gatttggtgt
    33061 tatgagaact aagactttta caatgaagga tgcatatagt tttgatgtag atgataaagg
    33121 tttagataag tcatatcaag atatgtttga tgcatatgta agtatatttg atagatgtgg
    33181 attggagaat agccctgtac aggcagattc aggagctata ggaggttcta catcagccga
    33241 atttatggtt aagtcagaag ttggtgaaga tgaagttgta ttttgtagtg gatgtgatta
    33301 tgcagccaat gtagaaagag cagaatcttg taatctagca tcacaaaaag aagaaatgaa
    33361 agaattagaa gaggttcata caccaggagc agctacaata aaagagttag aagaattttt
    33421 aaaaacttct ccagataagt ttgctaaaac tttagtatat gaggcagatg gaaaaacagt
    33481 tgtagtcgtt gtaagaggag atagagaagt taatgaaatc aaagtatcta atgccatagg
    33541 ttctgttata gaatttgctc ttgctacaga tgatgtagtg agaaaagtaa ctaatgcaga
    33601 agtaggattt gcaggtccaa taggtataaa tgctgactat gtatttatag ataaagaaat
    33661 tgttgaacaa agaaacatag ttgttggggc taataaaact gagtaccata taaaaaatgc
    33721 taactatgga agagattttg aaggaatagt tggagatttt agaaatgttc aagaaggcga
    33781 caagtgtata gtatgtggta agccacttga aatagctaga ggtgttgaag taggacatat
    33841 atttaaacta ggaactaagt actcagaatc tatgaatgca aacttcatag acaaagatgg
    33901 aaaatcaaag cctatagtaa tgggatgtta tggaataggt gttgaaagaa cagcagcagc
    33961 tataattgaa caacataatg atgagaaagg tataatatgg cctttgtcag tagctccata
    34021 ccatgttgtg atagttccag caaatatgaa aaatgaagaa caaatatcaa tagctgaaaa
    34081 tatatataat gatttgcaag ctatgggtgt agaagtttta ttagatgaca gagatgaaag
    34141 aataggtgtc aaattcaatg attcagaatt aataggaata cctatgagga ttacagtagg
    34201 taaaaatatt aatgaaggta aagtagaatt taaacttaga cataaagaag ataaagagat
    34261 aattgacata gaagaaataa atgaaaaagt aaaggcagag tttatcagga ataatgttag
    34321 gttaggataa taacttaaat aagatattgt atagataatc aaagttgtac tttatagaca
    34381 ccattttatc tatatataag gatataaaat ggtgtttttt tataaaacaa ataaatgagg
    34441 aggtaattaa aatggcaaaa aatgaaaaac aatttgttga agaaataaca aagatggaag
    34501 atgattttcc acaatggtac acagatgtaa taactaagac tgacttagtt gattatgcac
    34561 cagtaaaagg ttttatggtt gtaaagccat atggatatgc attatgggaa aagatgcaag
    34621 aatttatgga taaaaaattt aaagaaactg gacataaaaa ctgttatttt ccattattaa
    34681 taccagagag tttattaaac aaagaagcag aacacgttga aggttttgct ccagaagttg
    34741 catgggtaac acatggagga aataaaaaat tagaagaaag attatgtgta agacctactt
    34801 cagagactat aatatgtaca atgtatgcga aatggttaaa atcatataga gaattaccat
    34861 atctttataa tcaatggtgc tcagttgtta gatgggaaaa atcaactaga cctttcttaa
    34921 gaacatctga gtttttatgg caagaaggtc atacgttaca tgaaacagca gaagaagcac
    34981 aagaagaaac aatacaacaa ttagaagtat ataaagcgct atgtgaagaa ttacttgcaa
    35041 tgccagttgt agctggtcaa aaaagtgaaa gtgaaaaatt tgcaggtggt gaaagaacat
    35101 atactataga agctatgatg catgatggta aagcattaca atcaggtaca agtcacttcc
    35161 taggtcaaca ctttactaag gcttttgata ttacttttgc agatagagaa ggaaatttag
    35221 ctaatccata tcatacatct tggggagcat ctacaagact tataggtgga cttataatga
    35281 ctcatagtga taacagaggt ttagtattac caccaagagt agctccaatt caagttgtta
    35341 tagttcctat agctgctaaa aaaggaaatg taatggagac tgttgataaa atatatgctg
    35401 atttaaaagc taagggagta gctgtagaag ttgatgatag agataactat acaactggat
    35461 ggaaatttaa tgagtgggaa atgaaaggtg ttccagtaag agttgaaatt ggaccaaaag
    35521 atatagaaaa taatcaagct atggtattta gaagagatac tttagaaaag gattctatgc
    35581 ctttagaagg attagctgat gctatatgtg atttatttga tgttatacac aatgatatgt
    35641 ttgaaaaagc aagaaaacat agagaagaca atacatctat agttgaaaat atggatgagt
    35701 ttagaaaagc tttagaagaa aagccaggat tcataaagac tatgtggtgt ggagatgcag
    35761 aatgtgaagc taagattaag gaagaaacag gagcaactat aagatgctta ccttttgaac
    35821 aagaaaactt aggtcacaag tgtgtgtact gtggaaaaga agcagatagt atggtagtaa
    35881 tggcaaaagc ttactaaaat acatttaaat aaaaactggg gtagaaagta tttaatactt
    35941 tatcccagtt tttttgtttt ttatgagtaa taaaatgtat ttgtaactat gtcataagat
    36001 aagtcataga tacttatatt aaaaagaata taatagagaa ctatttttaa taattacata
    36061 ttaatgaaag caaaatattt ttgttatata gattttcaat aaagcaaaca ataaatataa
    36121 atgaatatta gtaaggagtg cttttaattt ttagaaaata aatgacttaa aagaaagatt
    36181 tatgaaatta aaactaggat tttctgatat aataaacatg tgtattttta aataaaaagg
    36241 atattattat tagatttgat taaaaataaa cttgcaaaac aatgtactag tttaatatta
    36301 tagttatatt gaataaaata tcaggaggaa gtaaaaatga atgtaagagt aaggtttgca
    36361 ccaagtccaa caggatttgt acatataggt agtttgagaa ctgctttata taactattta
    36421 tttgcaaaaa aaatgggcgg agaatatata ttaagagtgg aagatacaga ccaaagtaga
    36481 ttagtagaag gtgctataga gaatatgctt aatgctatga aatgggctgg ggtcaaccat
    36541 agtgaaggtg ttatattaga tgatagtgga aaagtagtac aaaaaggaga atatggtcca
    36601 tatattcaat ctcaaagatt agatatatac caagaatata taaagcaact tttagatagt
    36661 ggaaaagctt attactgttt ctgtacaaaa gaaagactgg atgaagtaag agatgctcaa
    36721 agagcagcag gagaaacagc taaatatgat ggtcattgta agaatctttc taaagaagaa
    36781 gtagaagcga atataaaagc aggaatacca tatgttataa gattaagatt gccagagaat
    36841 catacaatta agtttacaga cttagttaga ggagatatgg aatttaatac taatgactta
    36901 gatgaccaag tattaatgaa aacagatgga tttccaactt atcattttgc agtagttgtt
    36961 gatgactatt tgatgaaaat tacacatgtt ataagaggag aagaatgggt atcatctact
    37021 ccaaaacatg tttatttata tgaagctttt ggatgggaag cacctgtatt tgtgcattta
    37081 ccaaatatac ttaataaaga aaagaaaaaa ttaagtaaaa gacaaggtga tgtagctgtt
    37141 gaagacttta agaaaaaagg atatttacca gagggattag taaactatgt agcattagtt
    37201 ggttggtctc ctgaagataa tcaagaattg tttacaatgg aggaattaga aaaagccttc
    37261 tctgtagaaa gagtctctaa aagtggtgga gtatttgata ctgaaaaatt aaattgggtt
    37321 aatcagcatt atataaaaga tggtgatgat gcttatctaa cagacttagc aataccattt
    37381 ttaatagaag atggatttat aacagaagaa gaagctacta ataaatatga tttcttaaag
    37441 agtatgatat cagtacttaa agagaaatta caatatgtaa aagaagttac tgaacatgca
    37501 agtatatttt ttggagataa aatagaggta gaaactgaag agggaagaga gttcttaaga
    37561 ttagaacata tacctacttt aatagatgct ttgagagaaa aaatagaaaa aacagaggta
    37621 ttaaatgctg agtttgttca agctatgctt aaagaaatac aaaaagagta caaaataaaa
    37681 gggaaaaatt tatttatggg ctcaagaata attttgacag ggcaaatgca tggtccagat
    37741 ttaccaaaag taatggaagt tttaggaaaa gaaacttgct taaatagaat agcttatgta
    37801 aaaaataata tattatagtc ataaaagtcc gctaatttag aagcggactt tttttgtgtt
    37861 agtatttggt gaaaatatct gcttaagaaa atgaaggtat ttaaggtaat aaatttaata
    37921 tataaaaagc aaagcaaaaa tataaataaa atatgaataa taaactaata aaatattaag
    37981 catagaagaa gggaatctat tattttagtt tgatttaatt attaaggggg taattactat
    38041 gtatagtaat attgatgatg ttaaaaagga attaaaggag ctatgcttag aatatgttac
    38101 aattttagaa aaattaaaag atgaaaaaat gattacagaa gaaacttttg aaaagtgctc
    38161 ttctcaaaaa aaaatatttt tagaagaaca atagaggatg gtaatagtat tattattatg
    38221 tagtttgaaa gatagtacga aaaatgatag taatactatt atttattgca attttcaact
    38281 ataatataat atataatgtt aagaagataa tttataaaaa tacagtgagg tgatgagtgt
    38341 gaaagtttat aatacattga ctagaacaaa agaagaattt gttccattag aagaaggaaa
    38401 ggtaaaaatg tatgtgtgtg gtccaacagt ttataactat atacacatag gtaatgcaag
    38461 accatttata atatttgata ctctaagaag gtatttagag tatagaggat atgatgtaac
    38521 ttatgtacaa aactttacag atgttgatga taagattata aatagaagtc atgaggaggg
    38581 aatatctcct gaagaagttg cggctaagta tataaaagaa tattttgtag actgtgatgg
    38641 gctaggaata aaaagagcta ctgttcatcc tcaggttaca gataatatac aacagattat
    38701 agagtttata aaagagttgg aagataaggg atatgcatat gcagtaaatg gtgatgtata
    38761 ttttgataca aataagtttg aaggttatgg aaaactttca ggtcaaaaac aagaagattt
    38821 agaggcaggg gctagaattg aggttaatga ccaaaaaaga catcctatgg actttgttct
    38881 ttggaaggca aaaaaagaag gtgagccagg atgggatagc ccttggggag aaggtagacc
    38941 aggatggcat atagaatgtt ctgttatgtc taagagatat ttaggtgaaa caattgatat
    39001 acatgcaggt gggcaagatt taacattccc acatcatgaa aatgaaatag ctcaaagtga
    39061 agctagaagt ggaaaaacat tctcaaaata ctggatgcat aatggatata taaatataaa
    39121 tgatgaaaag atgagtaaat caaaaggtaa tttctttaca gtaagagata tatcaaaatt
    39181 atatgattta gagatagtaa gatttttcat gttatcagca cattatagaa atccagtaaa
    39241 ctttagtgat gagatgttaa atcaagctaa agcaggtctt gaaagactat ataatactaa
    39301 agaaaaatta gaatttacgc taagtaattt agtagaatct cctttaacag aaaaagaagt
    39361 agagcttgtt aaagaattag atgactttag acaaaaattc atagatgcta tggatgatga
    39421 tgttaataca gcagacgcag taagtgtaat atttgaatta gctaagttaa taaattctaa
    39481 tgtagatgaa aattcttctt tggagtttgc taaaaaatgc ttagatgaat ttaatgaact
    39541 tacaggagta ttaaatattg taaataagaa aaaagatact gtattagata aagacattga
    39601 agaattgata caaaaaagaa ctgatgcaaa gaaaaataaa gagtttcaat tagctgatga
    39661 tattagacaa caattactag atatgggaat tgtattagaa gacacaagac aaggtgttaa
    39721 gtggaaaagg atataaaaat ggaaaaaact gaattagtta ctatgtcacc attagtttta
    39781 gcatacttag gtgatacagt gtatgagaca tatataagag aatatttaat tagacagaat
    39841 acacaaagaa aagttaatga tttgcataaa ctagctataa agtatgtaaa agctaaagct
    39901 caggcaacta taattcatga aattgaaatt gagttaactg aagaagagag taaaatttat
    39961 aaaaggggaa gaaatcaaaa gtcaaatact tctcctaaga atgcagatat tatagactac
    40021 aaacatgcaa ctgggtttga ggcattagtg ggatatttgt atttaaataa tgaaatagag
    40081 agacttcaat atattatcaa taaaggaatc aaaattattg aaagagatat gtaaatatta
    40141 aagagtgttg tcttaataat aagtaataag gcaacacttt ttttataact aatgtataat
    40201 aaatatatag aaattagtaa tgagaaagga taaaaagtta tgaaagtaaa attaatatca
    40261 catacacctg aaccagaaaa agtaatagct atggcagcta agctatgtta ttcaccagtt
    40321 gggacagacg aaatagaaaa agacttaaca gatgaaagta tagagaaatt tttaaatatg
    40381 ttattaagta taggtcatgg gtcaatatta gaacatgctt catttacatt ttcaatagaa
    40441 ggtatttcaa gggcttgttc acatcaaatt gttagacatc gtatagctag tttttcacaa
    40501 caaagtcaaa gatatgttaa attagaacaa tttgagtaca taattcctcc agaaatagaa
    40561 aaaatagaaa aggcaaaaga gttatttata gactccatga aaaaagacca agagaattat
    40621 gataagttag ttgaaatatt gtttgaaaat cattataatg acctaataaa aaacggtaaa
    40681 aatgagaaaa cagctaaaag acaagctgaa aagaaagcaa tagaagatgc tagatacgta
    40741 tttccaaatg catgtgaaac aaaaatggta tttactataa atgctagaag tttgtttaat
    40801 ttctttgaac atagatgctg tgaaagagca cagtgggaaa taagaaattt agctgtggag
    40861 atgttaagag aggttaaaaa ggttgctcct atattattta aaaagaccgg tccaagttgt
    40921 gtaaatggaa gttgtccaga aggaactatg acttgtggag atatagttca agttagagaa
    40981 aaatttaagg ccttgtagga ggtgaataat ttggcgagta tagaaggaag aaatccggta
    41041 atagaagcaa taaaaagcga cagagaaata gataaaatat taattgcaaa ttcagctaaa
    41101 gaaggttcaa ttaagaaaat aataggaatg gctaaagata agaatataat tattcagtat
    41161 gttgataaac ataagctaga tgaagtaagt acaagtcatt cacatcaagg tgtaatagct
    41221 tatgcaagtg agtataagta ttatgaatta gatgagctaa tagatttagc aaagaataaa
    41281 gatgaagacc catttttcat tatattagat gagataacag acccacataa tttgggttca
    41341 ataataagga cagcagatgc agtaggagca catggagtta tcattccaaa aagaaggtct
    41401 gtacacataa ctcctgtagt tgccaaagca tctgctggag cagtagaata tatgccagtt
    41461 tgcaaggtaa ccaatatagt taatacaata aaaagactca aagaagaagg gctgtggatt
    41521 gctgcagctg atatggatgg agaaactttt tataaacaaa atcttactgg tcctcttggt
    41581 gtagtaattg gaagtgaagg ttttggaata tctagattgg ttaaacaaaa ctgtgacttt
    41641 atagtaaaaa tgccaatgat aggtaatgtt acatctttaa atgcatctgt agcaggtgga
    41701 atacttttat atgagatatt cagacaaaga ctggataaaa gtaaataggc atgaaaaaaa
    41761 atataaatca ttacttaata atagatggct acaatattat aaatgcgtgg gacaatttga
    41821 aagaactggc taaagaggac ttagaagatt ctcgagaaaa gcttattgat gacattatag
    41881 aattttctga gtttatgggt tataagacta taatagtttt tgatgcttat aatgttaaaa
    41941 attctcgaga aaaagttgaa aaaagaaaac acataacaat agtttataca agagaacacc
    42001 aaacagcaga tagttatata gagaaattta taacttcttt atcaaagtat gatgatgtaa
    42061 aagtagccac taatgactat gcagaacagc aaatgatttt aggaaaaggt gctactagaa
    42121 tgtctgcaag agaattaaag ttagagttag atcgttccaa gaataaaatg aaggaaaaaa
    42181 atatcggttt gaggaaaaaa attcagcgaa attggttgga ggaaaggtta gataaagaaa
    42241 cattgtcgaa acttgagaac attcgtagaa agcgttgaaa ttactaggtt ctttagacta
    42301 taatatacct ataaatttgt tttatatatg ggggagattt acatgttggt agcaaaagaa
    42361 aaaagttatg agttagtgga caattgtcag caggatgagt ataacattgt attaagagca
    42421 agtgaagggg ataaaatagc actagagtac attattacta aatacagaaa ttttgtaaag
    42481 gcaaaagcaa agtcatattt tttaattgga gcagataaag aagatataat acaagaagga
    42541 atgataggcc tttataaggc agttagagat tttgatggaa gtaagaccaa ttcatttaaa
    42601 tgttttgcgg agatatgtat aacaagacaa ataataactg ctataaaaac tgcaacaaga
    42661 caaaaacata tacctttaaa ttcatatgtt tctttgaata aaccaatata tgatgaagag
    42721 tcagacagaa cacttttaga tattatagct acaagtatag taacagatcc agaagagctt
    42781 ataattagta aagaagagtt aaaaaatata gaatcaaaaa tgaacgaatt gttaagtgac
    42841 ttggaattag aagtactaga attatattta aatggaaagt cataccagtt tatagcggac
    42901 aagcttaaaa gagatgtaaa gtcaatagat aacgctttac aaagggttaa gagaaaatta
    42961 gaaaaacatc ttgaaaacag aaatgattaa tagtaaaata cttaccataa gcttattgtg
    43021 agcaaaatta atttatatcg aaagataagg gaggaaattc aaaatggcta aagctaaata
    43081 cgaaagaaca aaacctcatg ttaatatagg gacaatagga cacgtagacc acggtaaaac
    43141 tacattaaca gcagcaataa caaaaacatt atatgacaga tatcaattag gagaagcagt
    43201 agatttcgca aacatagata aagctccaga agaaagagaa agaggaatca caatatcaac
    43261 agcacacgtt gagtatgaaa caccaa
//
LOCUS       DAMJWS010000026        41675 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMJWS010000026 DAMJWS010000000
VERSION     DAMJWS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.026"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            63..1607
                     /locus_tag="PC399_001476"
     CDS             63..1607
                     /locus_tag="PC399_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2592345.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKRNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
     gene            1783..2151
                     /locus_tag="PC399_001477"
     CDS             1783..2151
                     /locus_tag="PC399_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2592346.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            2153..2878
                     /locus_tag="PC399_001478"
     CDS             2153..2878
                     /locus_tag="PC399_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592347.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2872..3636
                     /locus_tag="PC399_001479"
     CDS             2872..3636
                     /locus_tag="PC399_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2592348.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3858..5108
                     /locus_tag="PC399_001480"
     CDS             3858..5108
                     /locus_tag="PC399_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2592349.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNIDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            5301..6185
                     /locus_tag="PC399_001481"
     CDS             5301..6185
                     /locus_tag="PC399_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2592350.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6544..7161
                     /locus_tag="PC399_001482"
     CDS             6544..7161
                     /locus_tag="PC399_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2592351.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            7269..9800
                     /locus_tag="PC399_001483"
     CDS             7269..9800
                     /locus_tag="PC399_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2592352.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            10234..10935
                     /locus_tag="PC399_001484"
     CDS             10234..10935
                     /locus_tag="PC399_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2592353.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            10985..12067
                     /locus_tag="PC399_001485"
     CDS             10985..12067
                     /locus_tag="PC399_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2592354.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            12328..13428
                     /gene="vanG"
                     /locus_tag="PC399_001486"
     CDS             12328..13428
                     /gene="vanG"
                     /locus_tag="PC399_001486"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2592355.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            13425..14231
                     /locus_tag="PC399_001487"
     CDS             13425..14231
                     /locus_tag="PC399_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2592356.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            14249..16387
                     /gene="vanT"
                     /locus_tag="PC399_001488"
     CDS             14249..16387
                     /gene="vanT"
                     /locus_tag="PC399_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2592357.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            16701..17321
                     /locus_tag="PC399_001489"
     CDS             16701..17321
                     /locus_tag="PC399_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2592358.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(17487..18023)
                     /locus_tag="PC399_001490"
     CDS             complement(17487..18023)
                     /locus_tag="PC399_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2592359.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(18136..18810)
                     /locus_tag="PC399_001491"
     CDS             complement(18136..18810)
                     /locus_tag="PC399_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2592360.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            19013..20887
                     /gene="asnB"
                     /locus_tag="PC399_001492"
     CDS             19013..20887
                     /gene="asnB"
                     /locus_tag="PC399_001492"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2592361.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(20979..22415)
                     /locus_tag="PC399_001493"
     CDS             complement(20979..22415)
                     /locus_tag="PC399_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2592362.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            22744..23322
                     /locus_tag="PC399_001494"
     CDS             22744..23322
                     /locus_tag="PC399_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2592363.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            23361..23939
                     /locus_tag="PC399_001495"
     CDS             23361..23939
                     /locus_tag="PC399_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2592364.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            24025..24654
                     /locus_tag="PC399_001496"
     CDS             24025..24654
                     /locus_tag="PC399_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2592365.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            24644..27403
                     /locus_tag="PC399_001497"
     CDS             24644..27403
                     /locus_tag="PC399_001497"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2592366.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            27490..29052
                     /locus_tag="PC399_001498"
     CDS             27490..29052
                     /locus_tag="PC399_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2592367.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            29053..30189
                     /locus_tag="PC399_001499"
     CDS             29053..30189
                     /locus_tag="PC399_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2592368.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            30253..31329
                     /locus_tag="PC399_001500"
     CDS             30253..31329
                     /locus_tag="PC399_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2592369.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            31307..32545
                     /locus_tag="PC399_001501"
     CDS             31307..32545
                     /locus_tag="PC399_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2592370.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            32599..33687
                     /locus_tag="PC399_001502"
     CDS             32599..33687
                     /locus_tag="PC399_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2592371.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLISKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            33715..34506
                     /locus_tag="PC399_001503"
     CDS             33715..34506
                     /locus_tag="PC399_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2592372.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(34621..35175)
                     /locus_tag="PC399_001504"
     CDS             complement(34621..35175)
                     /locus_tag="PC399_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2592373.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            35371..36573
                     /locus_tag="PC399_001505"
     CDS             35371..36573
                     /locus_tag="PC399_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2592374.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            36630..37610
                     /locus_tag="PC399_001506"
     CDS             36630..37610
                     /locus_tag="PC399_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2592375.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            37858..38811
                     /locus_tag="PC399_001507"
     CDS             37858..38811
                     /locus_tag="PC399_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2592376.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            38801..39757
                     /locus_tag="PC399_001508"
     CDS             38801..39757
                     /locus_tag="PC399_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2592377.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            39754..40509
                     /locus_tag="PC399_001509"
     CDS             39754..40509
                     /locus_tag="PC399_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2592378.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            40601..41566
                     /locus_tag="PC399_001510"
     CDS             40601..41566
                     /locus_tag="PC399_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2592379.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT        16190 a         4297 c         7215 g        13973 t
ORIGIN      
        1 aaacttttga gatgtactta tttgctaata aaatagaggt gttttaatgg gaggaaattt
       61 ttatgaggaa tttacaaaaa aaattgatgt tttttttatt tatttgtata attttacaaa
      121 ttacatacac aacaccagtc tattctttat gcaaactcta taaaaacaac tccataaata
      181 actttgagtc tattgtgtct aataatcagc ttattagcaa acatgcaata caaataatac
      241 ttattatggc tataatatgt gtaatactta tattctatat aatatatgat aaattaaatt
      301 ttaaaattaa attgcaaagg atagcatata cagataatct tacaggagca aatactatag
      361 ataaatttgt aattgatgct aacaaaatac tatgtaaaaa tactcaagta aagtatgctt
      421 tattatatat agatattgat aaatttaaat atataaatga tttatttgga tatgaagttg
      481 ggaatgaaat tttgcgtaat ttaacaaaga ttattaagag gaatatattt gaagaggaaa
      541 tgtttgcaag aatatctgcg gataatttta ttataattat gaaatatata gaagaagaag
      601 atataacaaa gaggttaaaa actatatttg aagaattaga cttgtttaat aataatcaag
      661 aagaaaaata taagttagtt ttaagttgtg gaatctattt tatacttcca gaagatagag
      721 atatcaactc aatcatagat agggctaata tacctcataa aatggcaaag ggaggacata
      781 aaagttcata tgcattttat gataataaga ttcatgacca agagataaaa gaaaaagaga
      841 tggaaaatac aatgttttca agtctagaaa ataaagaatt tattatatac ttacaaccta
      901 agatagaact taatacagga gagatacaag gttcagaagc acttgttagg tggaaacgtc
      961 ctgataaagg gctaatacct ccaaatgaat ttatcccatt ttttgaaaga aacggttttg
     1021 ttataaattt agatttatat gtgcttgaag aagtatgtat ataccttaga aaatggattg
     1081 atgcaggaat aaatccagtt actgtttcag ttaatgtttc aaggatacat ttatattgca
     1141 ataattttat agaaacatac aaaaatataa ttgataaata caatatacct gcaaagtata
     1201 tagaacttga attaacggag agtatcatat ttgataattt tgatatttta attgatataa
     1261 tgaataattt aaaaaaaata ggatttttaa tatctatgga tgattttggc tcagggtact
     1321 catcattaaa tatgttaaaa gaaattccta tggatatctt aaaacttgac caaaaattta
     1381 ttatggaaac ttataatagt aaaagaagca aaataattgt tactaaagta atagaaatgg
     1441 caaaagagtt gggtatgaaa gtcatatcag aaggagttga gacagaagaa cagtttaaac
     1501 ttttaaaaga agtaaagtgt gatatggcac aaggatattt gtttggaaag ccaatgccaa
     1561 ttgaagaatt tgaacattta attgtatcta acttggtaag agggtaatta atctgtaata
     1621 taaagaattt aataaattgt gttaaaaatg gactctaatg tagtgttcat tttttttgta
     1681 aaatttttta tacttttact aaaaagtaat tgacatatac tttgtattat tatattattg
     1741 tattaacaac ttaatacaat aatataataa ggaggcatga acatggaatg ggaacttgat
     1801 aataacaaac ctatatatat acaattagta gaacatttaa agttaaaaat aatttcaggg
     1861 gaaattaaaa taggatctaa attagaaaca gtgagagcac tagcagaaga tgcggaagtt
     1921 aatccaaata caatgcaaaa ggctctaaca gaacttgaaa gacaaggttt agtttatagt
     1981 cagagaacta aaggaagatt tgtaacagat gataaggaaa aaataaaagc tatgaaagag
     2041 gaaatagcta atgtggagat aaatacatta aaagtaacct tagaaaagct tggatatgat
     2101 agagatgaaa tgctaaaact tattacagaa aatctgaaag gagaattata aaatggataa
     2161 aaatataagc aatgtttctc aagatggaat agttgaattt aaaggtgtaa acaagagtta
     2221 tggaactaag aatgttttaa aaaatattga tttaaatata ccaaagggaa aaatagtcgg
     2281 gctgttagga cctaatggaa gtggcaaaag tacaatgatt aagctaatga atggattatt
     2341 acagcctgat aatggtgaaa ttatgataaa tggaatgaaa ccatccatag atactaaaaa
     2401 aatagtttca tatttaccag aaagaactta tttaaatgat tggatgaagg tatcagattt
     2461 acttaagttt ttctatgatt tttatagtga ttttgatgtt agaagagcaa atgagatgat
     2521 taaaagctta gatattgatg taaatgaaaa gctaaagaca atgtctaaag gaactaaaga
     2581 aaaagttcaa cttattttag taatgtcaag aaatgcgagc atatatatat tggatgaacc
     2641 tataggtggt gttgacccag ctgcaaggtc atatatactt aagactatat taaaaaatta
     2701 ttctgaagat agtactttac tgattgctac ccatttaata agtgaaatag aaaacatatg
     2761 tgatgagata atattcatat caaaaggaga aatagtgtta caaggagacg ttgaagcaat
     2821 aagagaagaa aaaggaaaat ctatagatgc attatttagg gaggaattta aatgttaggt
     2881 aaattattaa aatatgagtt aaaagcaagt ggaagaatat ttataccgtt atatatagcc
     2941 atattgatag tagctgtatt taacggaata tttatgaata caaatatact tcaggtacag
     3001 ggaataggaa tactagtatt aacaagtctt tttatggcat taggagtttt aactattgta
     3061 gttacaattc aaaggtttag aaaaaatctt ttgggggatg aaggttattt aatgtttact
     3121 cttcctgtta gcactagttc acttatatta tctaaatgta taacagcact tatatacgct
     3181 gtacttagtt ttatagttgc agtattcact tttggagttc ttatgctttt tggtacttct
     3241 ggaattttac tacctgaaat tttagatttg tttaacacta gttttaaatg gatttctgaa
     3301 aactttttag atattttatt attagtagta gtaatgttta tttcatattc atcatttatt
     3361 ttgttattat atacatcaat ctcaatggga caattaccaa aattcaataa acatagaaac
     3421 atagtggcat ttgcttcttt tatagctata aatatagtaa tatcaattgt tggagatgca
     3481 gttggaagca tcttaccaaa tgaagataca aatatggtat atcatcttta tcagagtcct
     3541 agttttatgc ttgcaatact tggtagtctt gtagttgcaa tagcgttatt tttcgctaca
     3601 aaatttatat tagataaaaa attaaattta gaataaatgt ttttatatgg agccatcttt
     3661 tgagtaatgt taaaagatgg ctcttattat ttatgtagtt aattcatgta tataatatga
     3721 atagtgtttt atttctagtt aaatatatac atatgttgta taatgatatt gcgtgtaaaa
     3781 atttgtaaga ataactatac actttatata agttatatca agaaaattta aaaatatatg
     3841 gatttgaggg agaagatatg aaaagttata caaaggctaa gtggagtgtt tggggtataa
     3901 ttacattttc atttgtttta gtattatttt taaggatgtc aactgctgtt gtttcagaca
     3961 atttagcaaa tgagttaggt tttaattcta tacaaatttc aaatatagca tccttttgcc
     4021 tatatgcata tgcatttatg caaatacctg caggaatttt gattgataaa tatggagcaa
     4081 gaaaaattag cagtcttgga ataataatgg caagtttagg gtcaatttta tttggtttga
     4141 ttcaaagtat agaattagca tatatttctc gtgtaattgt aggggcagga acatcagtaa
     4201 tattactttg tattttaaaa attcaaggga gatggtttaa taagtcagaa tttgcttctg
     4261 caacagctaa attttcattt gtaggaaatt taggtggagt tcttgctact tttccacttg
     4321 tgtttttgtc tgaacttgtt ggttggagaa attcattttt attaatcggt ataattggtg
     4381 ttgtaattgg atgttttatg tatattattg ttagagatac accaaaagag tatggattca
     4441 atatagatac tgaaccctat gaaaagtcag agaaggttaa tattgttgat ggtattaagt
     4501 ctgttataaa gaacaaatca acatggtata attcaatgat aatgttctct tttgtgggtc
     4561 ttacaagtgc atttataagc ttgtggggag taagatacat aatggacgtt tatggtgtga
     4621 gtaagagttt ttctgcgttt atagtatcat tttttacata tggatttata tttggttcaa
     4681 taattatgga ttttgtattt gctaaaataa ggtctagcaa atttaatata ataaaatttg
     4741 gggcaatgat agatttgttt atatggattg taattgtagt tgtataccag gtaaaaccac
     4801 caattatagt tttaccaata tcattcttta ttatgggatg tatagttatg tcacatctac
     4861 aggtttttaa tgatgccaag tataaaaata aagaaattta ttctggactg gcaactagtg
     4921 ttattaatac ttttgaattt ataggaagtg gaattattaa tttgattata gctatctctt
     4981 tacaagtaaa ttcatataat atagttgatg gatataaaaa aggttttgta gtatttatag
     5041 tactaagtat tataactata gtatcatctc atataggggt aaaaaatgat gattttaagg
     5101 ctatatagtg ttttattaat agtttaattt agtattatat tttgcatata tatataacaa
     5161 atagtttgca ttttcctaag atgtataatt ggaatataaa tcttgtttac tatgtacaaa
     5221 tggtattgta ttatattaaa tagaaaataa gaactttaaa ctataatatt ggttctaaaa
     5281 tataaggtaa ggagtttttt atggatacga cgaaaataga tgatataagc aagttagatt
     5341 taaataattt agatattaat aagatggata tgggaggtat aatggaaagt ctattagaat
     5401 gggctactac aagcggagta aaacttataa taggcatact tatactttct attggattta
     5461 aaataataaa aaaatttgta aaccatgtta tgttattact tagtaaaaga gatattgatt
     5521 taactttaag aaggtttttg aagtctcttc ttttgagtgt acttaaagtt gccgttataa
     5581 tcattgttct ggaatattgg ggaatgagtt tgtccagttt tgctgctgtg atagcatcag
     5641 caggggtagc aataggtttg gctttacaag gtagtctatc aaattttgct ggaggattta
     5701 ttatactatt gattagacct tttaaagtag gagattacgt agaagcagca ggccatggag
     5761 gaacggttga acaaatagga cttttttata cacaattggt tactccagat aataaacaga
     5821 tacttatacc taatggttct gtatcaaatg atagtctaat aaactattca gcaaagaata
     5881 ctagacgagt tgatttgact tttagtgtag ggtatgaaga tgatatacta catgttagaa
     5941 gagtacttaa agatattgtt aataatcata aattaataat aaatgaacca gaaccattta
     6001 taggagtagt agaacatgga gataatgcta ttaaatttgc aactagagtt tggtgcaaga
     6061 cagaagatta ttggacaatt tatcttgatt tactagaaga ggttaaagtt aggtttgacg
     6121 aagaaggaat tacgattcca tatcctaaaa tggatttaac ggtaaaagaa ttaaataaaa
     6181 tttaattttt ttatgtagtg tttaaattta agaatattat atagatatat ttataagatt
     6241 tgtgaaaaat taataagtcc tgttattttg taagtgaaaa tataaaaaat gggagtgtct
     6301 caaaatgaac tttttagttc atgaggcact ctttttgtat gaaatcaaat atatttttat
     6361 tatttcaaca ataaaaaaga ggtctgtttc tattttgaga caatattatt tttatgtaga
     6421 tttaatctta atgagtataa agttttaaaa ctactaaaat aggaggaatt ttaatataaa
     6481 agggtaaata aagggtttag agggcataat atagaataaa ttaattattt aaggagggtt
     6541 tttatgtgca aaaaaatgta tttattagtg gtaggtatac ttatagcagg tttattaact
     6601 gcatgtaatg ggagtccaac taaggattca gaacaagcac aagataatac aaaaaaggag
     6661 gcaaatgtat caaataacgc tttaaaagat gaaaaaaata atgaaaattt aatggaacaa
     6721 gattttaaag tgccatacac agatgcaata aacatattta aagacaaata caaagatgct
     6781 gatatagtag atttaagtct tgagagagat ttaaataaat ttgtttatac tgtggaaggt
     6841 gtagatgata ataatgaata taagatgaaa attgatgcta atacaaaaga tgtattagaa
     6901 gataaaacag aaaagttgga ttcagaagat ttaaatggag tcgctagaaa agaaaaatta
     6961 gattttaatg acataatgac acctcaacaa gctatggaaa ttgcattaaa agaacaaaat
     7021 ggaatggtta aagagtggag tttagataaa gatttagatg ttacttttta taaaataaga
     7081 atagataaag ataaaaatga atatgacata aaagtagatt ctaaaaaggg aactgtattg
     7141 aaggtagaaa aagaagatta gaagatttaa actatcttta aaataatttc ttttttgaat
     7201 aaaatttgtt taaaatataa caagtagggt ataaagagac taaataacta ttttaggagg
     7261 agtacggtat gtcattgaaa tcgctaaaaa taaaagaaaa tttatattgg gtaggttcgc
     7321 ttgaccctga tttaagagta tttgacataa ttatgtatac accttatgga actacttata
     7381 attcatatgt attaaaagga acagaaaaaa cagttttatt tgagacagtt aaagataagc
     7441 attttgacaa ttatattgaa agattaaatg atttaaatat agattttgaa aaaatagact
     7501 atattgttgt aagtcatact gaaccagacc atgcaggaag cgttgaaaag cttttggatt
     7561 tagctaaaaa tgcaaaagta gtagcttcag aaacagcaat taaatatctt aaggaaatcg
     7621 taaacaaaga ctttgaatat gttgcagtta cagatggaga tacattatca attggggaca
     7681 aaactttaga atttttctct gtacctatgc ttcattggcc agataccata tacacatata
     7741 taaaagagga taaaactctt gtaacatgtg attcatttgg tagtcactat agtaatgata
     7801 agatagtaaa tacacttgat gaagatgaag aaaaggatta cttggatgct ttgagatact
     7861 attatgattg tataatggga ccatttaaac catctatggt aactgccata gaaaagataa
     7921 aagatttaga tatagatact gtatgtccag gtcatggtcc agtattaact gaaaatccta
     7981 gaaaaattat agacctttat tacaactgga gtgtaaatga acaaataaag ttagaaaaag
     8041 aagttacaat ttgttatgtt tctgcacatg gatatacaaa gattatggca gaagctataa
     8101 aagcatatat tgaaaaaaat agcaactata aagtaaattt atttgatgta atagagcata
     8161 agcaagaaga tattttggca aaaatagcag tatcacaagg tgtattattt ggaacaccaa
     8221 caatattagg agatgcgtta aagcctatat gggatatatt aatatcgtta aacccagttc
     8281 ttcatggtgg aaaagttgct tcagtatttg gttcatatgg atggagtgga gaaggtatag
     8341 aaaatgctat ggagagaata agtcagctta gaatgacagc agtaaaacct tttgctgtta
     8401 actttaaacc atcaaatgaa gagattgata aattacattc ttatacaggt aaattcctag
     8461 ataagttaaa ttctactttt ggaagtaaaa agaaaactaa gaaatttaaa tgtgtaattt
     8521 gtaatgaagt ttttgaaggt gatagtgctc ctagtgtatg ccctgtatgt ggagctaagg
     8581 aagaccaatt tatagaagtt gaagaagatg aagttacttt tagaaaagat actgatgaat
     8641 atttcgtaat agtaggaaat ggagcagctg gtttttatgc agcagatgct ataagaaaaa
     8701 gaaataaaac ttgtaagata actatgattt ctaatgaaga tgagttgact tactatagac
     8761 ctgcactttc tgatggtata aatgaagaac ttggttcaga tttctacatg gaagataaag
     8821 attggtatga taaaaataat atagtagtaa tattaggaac gaatgtagat aaattagatg
     8881 aagtgaataa aactattata gtgaatgatg gagctattaa atttgataag cttgtaatag
     8941 ctacaggaag tagaaacttt ataccaccaa ttaagggtca tgatttagaa aatgtgttta
     9001 ctcttagaaa tataaaagat ttatatagtg taaaagaagc actagaaaaa tctaagaaag
     9061 ttgtagtgat tggtggcggt ttattgggac ttgaagctgc atgggaattt agactaaaag
     9121 gattagaagt agtagtagtt gaagccatgg atagcatact atcaaaacaa cttgataaag
     9181 aaggcagtaa aatattagaa caatgtgtaa gagatacagg aatagatgta agattgggtg
     9241 tagcagttga tggtattgaa ggtgatggaa aagctcaaaa agttgtattt aaagatggag
     9301 atagtgttga ctgtgatatg gtagtatttt caattggtgt aagagcaaac actcaaatgg
     9361 ttcaggatac atctgtaaaa attgacagag gtattgttgt agataagaca ttacagacaa
     9421 atgttaaaga tatatatgct tgtggagatg ttgcacaagt gggaaatatt agtttagcaa
     9481 tatggccatc ttcagtagaa atgggaaaaa tagcaggggc aaatgcaagt ggagataatt
     9541 tgacatttga aagtgaagta tatccagttt cattagatgc aatgaatgta aaagtattta
     9601 gtattggtaa tatacaaaat tttgataaag aaatttcatc aaaagatgaa ggtcaaagaa
     9661 tatataaaaa attatttatg aaagatggtt cattagttgg agctatactt ataaatgatt
     9721 taagctgtac tgttaaattg ataagattaa tatctgaaaa aggtgatttt gaggatatta
     9781 tgaaggctga tatactataa tttaaataca ttaaaataaa aaggagttgt cttaaaattg
     9841 atttttttct aatcaaattt taaattcaac tccttttttg aatttttata taaaataaca
     9901 tggaaatttt agtaatgtct gtggttacat acataggttt aattgttttt tatatggatt
     9961 gtctctggta taataaattg aattgtgttt gactgataga tatttttatt gaaagttaat
    10021 acctgttatg cgataataag gacgtgttag gggagttttt ataataacaa ttaatctcag
    10081 tttttgtgtt ccaatagtag catcaacaca ttctgatgta gtagttcttt tcttataaaa
    10141 attagttaca aatatgcttg taattatttg atatgtaaat ttaccaatat tataaatgat
    10201 gacctcgata ataaggagag tgaaaaataa tctatgaacg aaaatatttt gattgtggat
    10261 gatgaaaaag aaatagcaga tttaattgaa gtatatttaa aaaatgaagg ttttacagta
    10321 tacaagtttt atacaggtaa ggaagctcta gaatgtattg aatctaaaaa tttggatatg
    10381 gctattttag atattatgct tcctgatatt gatggatttc atatttgcca gaaaattcga
    10441 gaaaaatact attatcctat cattatgtta acagctaaaa ttgaagatat agacaagata
    10501 atgggtttaa ccataggtgc tgatgattat ataacaaagc catttaaccc tttggaagtt
    10561 gttgcaagag taaagacaca attaagaaga tatatgcgat ataataattc ttatgaacaa
    10621 cagtcgataa ttgttaatga gtatgatatt aaaggattga ttatcaataa agaaacacat
    10681 aaatgtagtt tatttgggaa agaagtagca ttaacaccga ttgaattttc tattctttgg
    10741 tatttatgtg aacatcaagg aaaagtggtt ccttctgaag aattgtttga agctgtatgg
    10801 ggagaaaaat atcttgataa taataacaca gttatggctc atatagggcg attacgtgaa
    10861 aaaattcatg aaccttctaa aaacccgaaa tttataaaaa ctgtatgggg agtaggttat
    10921 acaattgaaa agtaaaaata ttaaagtcaa tcctgacttt actcaagtga gaaataaaat
    10981 ttttatgaga acagctttaa tggcatttat agccattatt ttcatatgga ttttatattc
    11041 ttttatattg tatggtaagt tttctatctg gatagtatca ttctttgaaa atgcttttcg
    11101 tatggaatat aaagctgcat tgaatttata tcagcagatt ttccgtaata gtatggagct
    11161 cttcatgata ctagctatgg tgattgtgtt ttttataata ttccgtgttt atttaaattg
    11221 gtttacaaaa tattttagtg aaataaatca aggtattgac tctcttatca aagaagatgt
    11281 tggagaagtg gctttatctc cagagttatt ggcaatagaa aagaaaataa actcaattaa
    11341 acatatactt gaacaacgta aatttgagac acaaatggca gaacaacgta agaatgaact
    11401 tattgtttat cttgcacatg atttaaaaac accacttaca tcagttatag ggtatctgac
    11461 actacttcga gatgaaagtc agatttctga agaactgcga aaaaaatatt tatctatttc
    11521 gttggataag gcagagcgtc ttgaagattt aattaatgaa ttctttgaaa ttacaaggtt
    11581 taatttatca aatatatcac ttgaatacag tacagtaagc ttaactcgta tgcttgaaca
    11641 actaacatat gagtttaaac ctactttgat gagtaagaat ttaaaatata cactgaatgt
    11701 tacaccaaat atgatgataa ggtgtgatgt taataaaatg caacgtgtat ttgataactt
    11761 attacgaaat gctgtttatt atagttttga agatactact attgaaataa cagctgtaca
    11821 ggaggatgat tatataaagc ttaaatttat aaatcatagc aatacgattc cagaggaaaa
    11881 acttgagcgt atttttgaac aattttacag gttggataca tctcgtaatt caaacagtgg
    11941 tagagctggg ttgggacttg ctatagctaa agaaatagtg gagcttcata acgggacaat
    12001 aacagctcat agcgagaaca atataattac ctttaaggtt attattccga ttgtaggaaa
    12061 tttgtaagaa tttaagtata aaaaaatagg aaattcttta ggataaataa ataaagagca
    12121 ctcttgttat tgatataatt actgtatcaa aacaagagtg cttttatttt gatacagtaa
    12181 ttatatcaat agaaaaatgt gaaaatatat tatgtggaac agatgcaatt gtaagtgaag
    12241 gttgattgga tttgaaatag aaaaagtatt ttaacatgtt tctgtagatt gtataaaaat
    12301 aaataaatca acaaaggaga atcaaagatg aataataaaa aaattgcagt tatatttggt
    12361 ggaaactcaa cagaatatga tgtatcatta caatcagctt ttgcagtact tgagaatttg
    12421 aatacggaaa aatatgatat tataccaatt ggaattacaa gggagggtaa ttggtatcat
    12481 tatattggag attataataa tatccagaat aacacttggt ttgaaaggga tactgatata
    12541 attccagtta ttgtttcgca gaaccgtgtc aaaaatggta ttattgaatt tttaattcct
    12601 gatgctgttt ctcagaatca tattccaaat agtgctattg aatcatgtga aaatgatatt
    12661 aaatacacta aacttgattt tgtgtttcct gtactgcatg gaaaaaatgg agaagatgga
    12721 acagtgcagg ggttatttga attggcaggt atacaggtta ttggatgtgg tactctttct
    12781 tcagcattat gtatggacaa agacaaatca cataaacttg ttcattcaga aggtattaaa
    12841 gtaccaaaat caatattact aaaatactca gataaattta atgtggctga actgtcaaaa
    12901 gacttaactt atccattatt tataaaacct gtgcgtgcag gttcatcatt tggtattagt
    12961 aaaatttata aaaaagaaga gttacaagta gcagtagact tggcttttga acatgataat
    13021 gaagtaataa ttgaggaaaa tattgatggt tttgaggtag ggtgtgcagt tttaggaaac
    13081 aatgacttac ttgttggaag agtagatgaa atagaattgt caaaaggatt ttttgattat
    13141 acagaaaaat atacattgaa aagttcaaaa attcatatgc cagctagaat tgatttggat
    13201 tgtgagaaaa gaatacaaga gacagcaaaa attatttata gaactcttgg atgttctgga
    13261 tttgctagag tagatatgtt tttaactcca tctggtgaaa ttatttttaa tgaagtcaat
    13321 acaattcctg gctttacttc tcatagtcgt tatccaaata tgatgaaagg tattggactg
    13381 tcttttgaag aaatattgga caagataatt gaggtgtaca taaaatgatg aataatattt
    13441 tattagataa aaataaagaa gataaaatga caaatactat cttattgact aaagataaag
    13501 tttatgatgg gaatctaatt ctagtaaatg cttttttacc agttaaaaca tctgaagata
    13561 tagatttaat tcctgtagat acaaggtttc caagtattct tatgaaacgt gaagctacaa
    13621 acattttaca aaatatttta aaaagtatat gtggaataaa tgaaatagta cctgtaagtg
    13681 gatatcgtac tgcagaggaa caacaagata tatattcaag ttctctgcgt gacaatggaa
    13741 aagactttac taaaaagttt gtagcacttc caaatcacag tgaacatcag acagggcttg
    13801 ctattgattt agggttaaag aaagaagtta tagattttat ctgccctgaa tttccttata
    13861 atggtatttg taatgagttt cgtaaggcag cactacatta tggttttata gagcgttatc
    13921 agtgtaaaaa agaagaaata acaggaatat ctcaagaacc atggcatttt cgttatgtgg
    13981 gatatccgca ttctgagatt atcaataaaa tgaatctcgc tcttgaagaa tatatagaat
    14041 atattaagag attttcgtat acaaattgtt taaaaataga agataaagaa aaaattatag
    14101 aaatctttta tgtgcctatt catactaaag aagaagtgag aatctctata cctgaacaat
    14161 gtgtatatca aatatcgggt aacaataagg atggttttat tgtgacattt tggagaaata
    14221 gaaaggacta agaaatggag gaacaagaat gattaggaat aaatcttatg cagggcttga
    14281 ttattttagg attatagctg cattacttat aattgcaatt catacatctc cattattatc
    14341 atatagtaaa atggctgact ttgtgttaac tagtataatt gctcgtattg ctgtgccatt
    14401 cttttttatg ctatcaggct tttttctaat ttcaacatat aattataatt gtaataaact
    14461 gaaatctttt ctaaaaaatg tattatttat ttacgggata gctattgtaa tttatattcc
    14521 cttaaatatt tacactggat atttttccat gaataattta gtgcccaata ttattaagga
    14581 cattgtattt gatgggactt tatatcattt atggtatctt cctgcatcaa ttattggtgc
    14641 aagtattgca tactttcttg taaaaaaact aggtttaaag tactcactag taatcactat
    14701 tgttatttat gtaattggta tgtttggtga tagttattat ggttttttag aaaaaatacc
    14761 actattaaag aatttatatg ggtatatttt tgaagtatca gattatacac gaaatggtat
    14821 attttttgca cctgtatttt tcatacttgg tggaataata tctaataaat ctaattatat
    14881 gtcaactaaa aatagcttga ttggatttct tatatcattc ttacttatgt tatgtgaggg
    14941 aatgttttta cataaactag gggtacagcg tcatgatagc atgtatatta tgttattgcc
    15001 atgtatgtat ttcttattta cagccttaac atttttgaaa ggttctcgag ctaggttatt
    15061 gcgaacatca tcattaatta tttatattat acatcctatg atgattgttg ttattcgtat
    15121 gttttctaaa atattcggaa tacaaactgt cttggttgac aatagcctta ttcattatat
    15181 agtggtaagt atagcatctt ttatagcttc tataattatt atattgattc ttaaacctct
    15241 aaaaaaagac ttgaaaggag aacataaggt aaataccaat agagcttgga ttgaaattga
    15301 tttaaataat ttggaacata atgtaaagat gatacaaaaa gctatgccag aagattgtga
    15361 attgatggca gttgtaaaag ctaatgccta tggtcatagt gcttttgaag tagcgacttg
    15421 tgcaaaccaa ataggtgtaa aagcatttgc agtagcaaca atagatgagg ggattgattt
    15481 acgtcgatat ggaatactag gagaaatatt gattcttgga tatactaacc caataagggc
    15541 aaaggaactt cataaataca atcttacaca gagtgttatt gaccttaatc atgctatctc
    15601 tttaaacaaa cagaaataca acataaaatc acacattaaa attgatacag gtatgcacag
    15661 actgggtttt gatgttaatg atgtgaggtc aattttaaag gcatttaaat taaaatactt
    15721 agatatatgt ggcatattta cacacttgtg tgtttcagat agtctgaaag atgaagatgt
    15781 agactttaca aataaacaaa ttgaatgttt ttataagtta attgattttc tgttaaaaaa
    15841 aggtataaaa attcctaaag tacatattca aagtagttat ggttttttta attatcccaa
    15901 tcttaagtgt aattatgtaa gaattggaat tgcattatac gggatattaa gttttccaaa
    15961 tgatacgact aaattgcaac ttgatttacg acctgtttta tccttaaaat cacaagtaat
    16021 tcttattaga aaaatacaaa aagatgatag ttttggatat ggaagagtct ttattgcaag
    16081 cagagatagc gtaattgcta tattatctat tggctatgcc gatggtatac ctagaaattt
    16141 atcctgtgga aaaagttacg taataataaa tggttgtaga gcagcaatag tgggtcgaat
    16201 ttgcatggac caacttgctg ttgatattac agatatacct aatgtagaag ttggtaatac
    16261 ggcaataata ataggaagag ataatttatc tgaattatct gtttctgaag ttgcaaataa
    16321 ctcatgtagt atatcgaatg aattattgag cagagtagga agaaggttaa atgttataaa
    16381 gaagtaactc aattaaatag taatatttgt tgtactgtgt aatttatttt actctgacta
    16441 gacattttta gtaatattat gtctaatata tattttttat aacttacatg acaatttaag
    16501 ttataaaaag gtgatgtttc agatttttat tttgaaacat cacctttttt gttataatat
    16561 ataataaaat aattatacct taactgtaaa cttatataga ttatttactt tttaatatta
    16621 gattcatttt ttgagattgg tatataataa acatatagag tatataataa tagttaaagt
    16681 taaaaaaggg gaaaagcaat atgcagtata tacaagaact acttgatttg actcaaaatt
    16741 attgggtgtt agccacaata atcggcttat taagtgcatt tatagaaagc tttataccag
    16801 ccttaccact tgttgctata gttacagcaa atgctgcaat acaaggatta tttattggat
    16861 gtttgttatc atggattggc tcaggacttg ggacaacttc attattctta ttaataagca
    16921 gatttaatga tagtaagctt tttaataagt tgagaaatag taaaactgaa aaagcaataa
    16981 gttggatgga taaacaggga tttaagttat tatttatagc gtatgcctgt ccatttatgc
    17041 ccggttgttt agttactatc gcatcagctt ttggtaaaaa agatttaaaa gattttgtgc
    17101 cagcaatgtt ggcaggaaaa tttgtaatgt ttatagttat aagttacgtg gcaagtgata
    17161 tagaaggttt tataacaagt cctttaaaga tagcatcatt tatattattg gtgtttttgt
    17221 catggaagat tggaaataga gtaaataaaa atcttgaaaa tcataattat gatttccatc
    17281 ataaaaaaca tcacaatgat aaagatgata aaatgattta gcttgtacga aataaaacgt
    17341 atcttaaaat gatttagtac taaattattt taagatacgt tttatttttt gtattaaggt
    17401 acaaaatttt tagttctttt gtaaactaaa atatataaaa cttatttata cttagttcaa
    17461 aatattgtta tttatttttt ctacaactat aataaaatat aaaattgata tccaatcaaa
    17521 aaaataacta tgaaatttaa tagcttagat atgaattttt ctgtttttga gttagttttt
    17581 ataggtagta aagaaatgtt tatagtaatg ggataaaaca attcaatacc ttctttagta
    17641 ataaggtcaa gacacaagtg agatagatat cctattataa acccactgga caaacatgag
    17701 aaaactatat tattgttagt gaagattgtc agagatttaa aaaaactata aataagtaat
    17761 aaggcaatta gactgtgagt aaaccctcta tgtctaaacc tacttccaaa taatttgtat
    17821 attaacataa attttttact tatgtaagaa cctctcatat ctatatctgg aaataaagaa
    17881 cctaaatatg caaaataaat ataaattata attaatacta ttttatatgc aatatcgtat
    17941 ttaactaggt acagattgtt tataaagggg agtatcaaaa gtgcgaatat ataacctccc
    18001 ctttcatgtg tttcttttat catataaaaa tcctcctgaa tcgtcactat aaacattata
    18061 ttggtaaatt tagactatct tttattatat tatatagcaa acataagttt ttttcaaaag
    18121 gattttaata tttaattaat cttgagattt taaatttttg taattttcta aagcaccatt
    18181 ccaatttact acattaaacc agttgtcaat gtattcattt cttctatttt gatattttaa
    18241 atagtaagca tgctcccaaa catcaagtcc tattatagga gttaggtttt tacttacagg
    18301 gctatcctga tttggagtag tcataataga taatttccca tctttagtag ctacaagcca
    18361 agcccaacca gaaccaaaga catctaaagc agatttttgg aactcttgct taaatttttc
    18421 aaaagaacca aagtctctat caatagcttc ttttaaagat tcagaaggta tggttttttc
    18481 tggcgtcatt atatcaaaaa agaatttatg attataagct ccacctgcat tatttcttac
    18541 agttgtagca atatctttag gtaaagaatc taaattttgc aataattcac ataaagaata
    18601 attataaagc tcaggatatt tttcaagagc agcatttaat ttatcaacat aagcttgata
    18661 atgcttatca tgatgcagtt tcattgtttc tttatctata taaggttcaa gtgcatcata
    18721 tgcataaggt aatggtttaa ccttaaattt gttattttca ggtgtaaaag caaatgaagt
    18781 tatgcactgt gagattataa ataaagacat aataactggt attaatattt ttttcttcat
    18841 aaaaatcacc tcctaatata gtatttctag aaataaaaaa aatatgaaat attaacagat
    18901 aatttataag aatttaatag caatttcatt ttaaattgaa taagttttaa agccaaagac
    18961 aaaactatga aatatagaat aagactaaag gacagaaagg aattttgatt atatggacgg
    19021 caatatttca atttattata aaggtgatat atctaaaaaa gaagatatta aatttaaaat
    19081 aaaagaatct gtaaataata caagactttc tagtagaata gagtataagg atgaggaaaa
    19141 aacttttgat tttgaaagat ttaatgtaat tgatgctatt tatggaaata aaccatttga
    19201 aaaagatgga aaagttattg tttttaatgg aaatatatat aactatcatg aaataaaaga
    19261 agaattaatt aaaaaaggac acaattttac tacaaatgat gatattgaag tcttattggc
    19321 atcttatata gaatttggta agaattgtgt acataagatt aaaggaatgt tcaactttat
    19381 aatctatgat agagaaaatg aaagtatatt tggagcaaga gatttatttg ggataaaacc
    19441 actctattat ataaataagg aaaatgcaat aattttttcc tcagagtata agtttttact
    19501 tgaatatatg aaaaatttaa atataaatga aagaagctta caaagttatt tttcatttca
    19561 atatgtactt cctgaagata ctatgataca aggaataagg ctgataccag caggtcacta
    19621 ttttagggta gaaaatggta tactatcttt aaagagatat aataaacttg agtttagaag
    19681 tagtacaaaa tttttttata ctaaaaatca cttaggcaat agagatgtga acgaggatga
    19741 tataagaaat atagtagtag attcaatagt aactcatatg gaagaggaga aagaaattgg
    19801 aacatttctg tctggtggaa tagattcatc tattataact actgtggctt cacaaattaa
    19861 tccaaatata aaatcttttt ctgcaggctt tagtgtcaaa ggatatagtg aattagaagt
    19921 agcaaagaaa acagctgata agttaggtat agaaaatatc cagattaata taacacaaga
    19981 tgaatatata aaatcattac cgaatgtaat ttattctttg gatgacccta tagcagaccc
    20041 ttcagaagta ggtctttatt tcttaattaa agaggcaggt aaacatgtta aagtagcatt
    20101 gtctggagaa ggagcagatg aattatttgg aggatataat atatataaag agtatagtac
    20161 tatgaaatca gttgtcaatt cacctactta tattaaaagt atattaggaa gagtttcaga
    20221 acttatgcca aacataaaag gaagaaatta tctttataga gctacaacac cattagaaaa
    20281 aagatatata ggaaatgcaa aagtttttga aaacagcgaa gtaaaaagat tcttttttaa
    20341 gtataaggaa aagaatatat atgaatacct gttgtctaat ttgtatagag atgcacaaaa
    20401 aaataattac gattatataa gtaaaatgca acatatagat gtgaatacat ggcttcaagg
    20461 cgatatactt caaaaaatta gcaaactttc tacagctgag caggttgaac ttagagtacc
    20521 atttttatat aaagatgtat ttgatgtagc aaaaaattta agaatggagc aaaaaataaa
    20581 taagaataat acaaaagttt tacttagaga ggcgtttaga gaaatagtcc cagaacatgt
    20641 tgttcaaagg aaaaaattag gatttccaac tccaataaga gtttggttaa aagattctct
    20701 aggggatatt gtaaaggaaa ctatttataa ttcaaatgta gatgaattta tagataaaaa
    20761 atatgcaata aaacttttag atacgcatct taaaggtcat agagataatt cgagaaaaat
    20821 atggactata tttacatttt gtctatggca tcaattattt attgagcata aaaatgtcga
    20881 gtattaatac ttttttcaat attaatcaga gggcaaaagc atgcaatgtt ttcaccctcc
    20941 tgattattat ggaataggat aagtagaatt ttgattgatt agtttactat ctcaataaaa
    21001 ttattggcaa atattttgac aagattactt tcaacagcta aatttaagtt ttgtccatca
    21061 tcatatccag cggaaatcaa tcctagtaag tttccaaaca tatctaaaag agctcctcct
    21121 gaactaccac tagagattgg agctgtaaat tgtatcattt gaaccgtttc aaaatcacga
    21181 aaagctgaaa taattccatc tgaaactgta ttaaaaagac ctagagggct tccaattgca
    21241 actatttttt gacctctcac aggtttttta ctgactttaa caggtattgg cttgcatttt
    21301 ctatcaacct ttatcacagc taaatcataa tctgaatggt attttataat ttggtaagat
    21361 gtataaactt tatcatcatt ttcaaatcta actaaaaatg aatacccatc attgactaca
    21421 tgaagattcg taagtatata accttcatta tttattacta ctccagaacc gctcttaaac
    21481 ggtttatttt cgttattgca cacaacaatc ataacaacag aagctgataa ctttgcaagg
    21541 tcttcataag aaagttctac ttcctcatct tgtacaattt ttttagatgt tagatttgtg
    21601 ttattaagtg tatttttaga gttaggttta ttcgtagtag gagaagtctt attggtattt
    21661 tcactgtaat ttatatttgt aatatgcctt tcaaaaggta tttctaatat atttggagtc
    21721 ttcctagaaa gttcaaatag agtagaaaat tttgaacatt caggatataa atcaagaata
    21781 tctttatttg gatttatata taaaatatca cacccaagtt gtgataaaaa atataagaaa
    21841 aaaagttctt gccttttaat atttccaaag tatactatct ttggtgaaat agtagtcttc
    21901 accttgttat aaaataattt aggtagaaaa tagtctatcc aacacaaaag tttaactgca
    21961 aagtttttga tgatagagga tgtacaattt ggcatacttg tggaaaataa atctaaagta
    22021 tttttaaatg caatcttttt agtccaattt aaggtgtcat tttctatttt acatggaaag
    22081 tcgatattat ataaatccca actgttaaac ttagaactat tataaatatt aaaaatttta
    22141 agcatcttat ctgtttgttc tttgtcaaaa tttattttta gattgtttaa tctaatatag
    22201 ggaattgtag gattattaaa aaaataagta tctatatttt taattttttc tatgtattta
    22261 tcaattgaat catcaacacc tatatatctt gtaaagtaga cttctttaat atatgaagaa
    22321 cgcttatttt tataggtagt atcagatata aattcaatta gaggttcagt agactttttt
    22381 attgaaatct ttaatggttt aatagtacaa atcatatttt ttctcctata ataagatgat
    22441 ataatctatt ataaaacaac ttaaaacaat atagtacaat aattttgtag tttcaaaata
    22501 aaatatgtac ttgtttatat aaatgatata taattacata ttaaacatag cttttatgca
    22561 tttaataaag tatgtaaaat ctatgtaaaa agtaagaaat ataaacattt ttttaattat
    22621 attttaaaaa tatcaagcaa gttttattca caaatgaata aaaaatgtta taatatgatt
    22681 aaagaaaaag ataaataatt agcaagattt gtttagctaa aattaacaag ggagcgataa
    22741 attatgggta taacattatc aaaaggccaa aaggttgatt taactaaagg aaatccaggt
    22801 cttaaaaata ttttagtagg tttaggatgg gatactaaca agtatgatgg tggatttgac
    22861 tttgatttag atacagcagc atttttaaca ggagcaagtg gaagtgttac taatgatgga
    22921 gattttgtat tttacaacaa tttaaaacat acatctggag cagttgagca tctaggagat
    22981 aacagaactg gcgaaggtga tggagatgat gaacaaatag ttgttgattt atctaagatt
    23041 cctggagaaa taagcaagat ttcttttact gttacaattc atgatgcaac agaaagaaga
    23101 caaaattttg gacaagtaag taattcttat ataagaatag tagataaaga cacaaatgaa
    23161 gagttaataa agtatgaatt aggagaagat tttagtatag aaacagctat agtagtagct
    23221 gaaatatata agcacaatgg agaatggaag tttaatgcac taggttctgg ctttgaaggt
    23281 ggtcttgctg cattatgtgg aaactttgga ataaacttat aatttcaact ataacaataa
    23341 actaaagggg gatattttcc atggctatag tattaaaaaa aggacaaaaa atcgatttaa
    23401 ctaagggaaa tccaggtctt aaaaatataa aacttggact aggatgggat acaaattcat
    23461 ttgatagtgg ctatgattat gatttagatg taagtgtttt catggttgga gaatctcaaa
    23521 gagttgaaaa agatgaagat tttatattct acaataactt aaaacatcca tctggagcag
    23581 ttgaacactt aggtgataat agaactggtg aaggtgatgg agatgatgag gaaatattag
    23641 tgaatttaga gacaatgcca aagcatatac aaaagatagc tgtaacagtt acaatatatg
    23701 aggctgctga aagaagacaa aattttggac aagtaagcaa ctcatatata agagtattaa
    23761 attctgaaaa tgatgaagaa atattaagat atgacttagg tgaagaattt agtatagaaa
    23821 ctgcaattac agtatgtgag atatataagt ataatggaga atggaagttt agtgcagttg
    23881 gagcaggatt tgaaggtggc ttagaagcac tttgtagaaa ctatggctta aatgtttaat
    23941 ttaagcaata taatatatta aatataaata acctatacaa ataatgtata ggttatatta
    24001 tataaaattt tgtggaggtt tagtatgtcg attaatttat caaaaggtga taaaatagat
    24061 ttaaaaaaat ctaacccagg actgagtaac atacttgtag gtctaggatg ggaccctgtg
    24121 caacaatctg gcggaggttt ttttaaatcg ttatttggtg gaggacaagc tgatatagat
    24181 tgtgatgcat ctgtatttat gcttaatcaa gaaggtaaat taagtggaat aaaagattta
    24241 atttattttg gaaatctaaa aagtgcctgt aaatcagttt tgcatacagg agacaatcta
    24301 actggtgagg gtacaggtga tgatgaacaa atcctagtta atttggataa agttccatca
    24361 aatattcata aattattgtt tgtagtaaat atatacaatt gtgtagatag aaaacaacac
    24421 tttggaatga tagaaaatgc ctatataaga gttgaagacc aaggtaataa gaaagagata
    24481 gcaaaataca atctatctga taattattct gaaaaaacta ctcttatagt aggagctata
    24541 tatagaaaag atggaagttg gcaatttaaa gcaattggtg aaggaacaaa agatgcgggt
    24601 cttaaagaag taatgcaaaa tttagacagg attgagtgtg catatggaat ttaataatga
    24661 tatcaaatat tataatgtag ataaagaaga agttacaaaa gacttatctg ttaatcctga
    24721 aaaaggttta tcagaatcag aagtcaagac tagaagagaa aaatatggtt tgaatgaatt
    24781 tactccaaaa gaagagggta gtttttggga tgatttaaag gaaagtttaa gtgaaccaat
    24841 gatagttatc ttgattattg cagcagtagt aagtgcagta attggggaaa ctcatgatgc
    24901 aatagggatt gtaggtgcta tagcaatagg tatagcaata ggtatgatta cagaaggtcg
    24961 ttctaagaaa gcagcagagg ctctttctaa gttgactgaa aacatagaag taaaagtatt
    25021 gagagatggg gaagtacatc aaatttctaa gagtgaattg gtaccaggtg atatagtata
    25081 tattgaaact ggtgacatgg tacctgctga tggtagactt atagaaagta taaaccttaa
    25141 aattagagaa gatatgttaa caggagaatc tgatgacgta tctaaaaagt gtgatgtagt
    25201 agtttctatg gaagagatag aatctaaagg agcagtagta gtacaggaac caatacctgc
    25261 aaaacagata aatatggtgt ttggtggtac acttgtagct tatggtagag gagctctagt
    25321 tgtaacttca attggagata atagtgaaat gggtaaaatt gctcaaaact tatcagaaac
    25381 agatgagcaa actccacttc agataaaact tggaaattta ggtggtttga tagccaaagt
    25441 ttctagtgca gtagcaggat tactatttat atttatggtt ttccaaatga taagtaagaa
    25501 tgtattaaat gtagatatgt ctggaatatt accgttttta gaatcaatag acccagttaa
    25561 aacagcattt acagtatgtg ttgcgttgat agttgcagca gtgccagaag gtcttccaac
    25621 tatgataaat atgacacttg caataacaat gcaaaaaatg gctaaaataa atgcattggt
    25681 taccaaaaaa gaagcttgtg aaacgatagg ttctgtttct gtaatatgtt cagataaaac
    25741 tggtacatta actgaaaata gaatgactgt agaagttgca tatgttgatg gcagatatat
    25801 tgaaagttca gaagaggaaa taaatagtta ttttgaagaa aactgtatga taaactctac
    25861 agctgatgta gagcacaatg atggtgatat taagtattta ggaagtgcta ctgaatgtgc
    25921 attgctttta tattacaaaa acgtagatta tagacaagtt agaaaaaatt ctaatatagt
    25981 agctcaaaat ccttttactt cagattcaaa gagaatgact tctgttatag gtcaagataa
    26041 tcatcatgta ctattatcaa aaggagcacc agaagtctta cttgaattat gttcacatat
    26101 tcaaagagga aaagacatag ttccattaac tgaaaatata aaacatgaga ttttagaaga
    26161 aataaagaaa ttgcaaataa aatcaatgag aactttaggg tttgcatata aagaaatctc
    26221 acaagctgaa gaagaagcag cagtaactgc tgaaagtgat gctatgaatg taagtgcaat
    26281 ggaaaacaat ttagtattta gtggttttgt tggtataaga gacccactta gaaaagatgt
    26341 tatagagtct gtaaatacag caaataaagc tggtgtttct gtaaaaatgc ttacaggaga
    26401 taatataaat acggcaagag ctataggtga agagttaggc ctattaaaaa ataatatgag
    26461 agtggttgaa gcttcatata tagatacttt agatgatgaa gaattaaaac aagagataca
    26521 aagtatttca atcgtggcaa gaagtaaacc agatagtaaa atgagaatag tttcagctct
    26581 gcaaaagagc ggagatgttg ttgcagtaac aggtgatggt ataaatgatg ctccagcact
    26641 ttcaaaagca gatgttggta tagcaatggg tatatctggt acagaggtat ctaaaaacgc
    26701 agcagacata attttaactg atgatagttt tagtaccatt gtaaaaggta ttaaatgggg
    26761 tagaggaatt tatgataact tccaaagatt cgttcaattc caattaactg taaatgtaat
    26821 tgcattttta gtggctataa tatctcaagt tatgggacaa gagatgccat ttactacaat
    26881 acaattatta tgggtaaaca tcattatgga tggaccacca gcactagcat taggtttaga
    26941 gccagttagg gaccatgtgt taaacagaaa acctgttgat agaaaagcca atataatttc
    27001 taagtcaatg gtgtatacaa tagtattaaa tgcattttat ataacagcaa tactaatgtt
    27061 acaatctaca tttaatttct taggagttga tgaagcgcat aagggaacag taatgtttgc
    27121 gttatttgct tttagtgcac tgtttaatgc atttaactgt agagagttta atgcagatag
    27181 tatcttccct aattttacac ataacaaatt agcactacaa ataatagtgt tgacaggaat
    27241 tgcacaagta atatttactc agctattcca agatttcttt aactctgtag caatggattt
    27301 tatgacatgg gtaaaagttt tatgtgtagc agcaagtgta attgtggtta atgaagttgt
    27361 aaaatgtata ataagactgg taaaaccaaa aaagactgct taaaacaaat tttttagaag
    27421 aaaacagact gactcaaaat atgaattttg ggtcagttct gaataaacat ttaaattggg
    27481 aggatagtta tgggtgactc aaaggtattt gaaaaaatat tctccagcca atcagataga
    27541 ggaaattaca ctccttcaaa gggatatcta agttatttta tttcgtatat tggtttagaa
    27601 gatgaagttt tgtacaatct cgagatattt aaaacaaaac aaaatattga tagcaaaaaa
    27661 gatattgctt tatttactga tgttatagca aatcctagtg attttgatat tataaattat
    27721 tttaaaagtg gacttcaaaa atatagaact agcatggaag atgtagatat aaatattttg
    27781 ggatttgaag agattgatta taaaataaag caagcaatgg atagagtgtt aaaagaagag
    27841 gaaaaagaat ttactaatga tagagttaaa caaaatttta tagtgaaaat aatggcatgg
    27901 ataaaaattt atatcggtgc tttagatata aataaaaatg aagctccaaa agtaatattt
    27961 tatggagata taaaaaaaca tgaagtttat ttactcttaa tattatactt ggcaggtttt
    28021 gatgttttat atcttaatcc aaattcaaag agtaatatag atattttaaa atcagaaaga
    28081 tacaatatag agtttgaaga agccaacata atagaagaaa aaatttcttt tgaagaaagg
    28141 gttattttag gggaaaaaat agataaaagt tctgtaaaaa aagcttttac tgttggggca
    28201 gaagcatcaa agcgtataag tgaggaattg ttaaatgatg caggatttat aaaaccttgg
    28261 caactccaag atagaaaaat aaaaaatcta cttctaagtt caactgttga tgaaatatca
    28321 atatactgga atcaaccact taaattaaga ccaggattta agtttaatga tgctattgta
    28381 gaagctccaa attttctttc taagattaat ggaatatata acgataagaa tgaatatatt
    28441 aagtttttag atttattaag agattcggaa agttcaacat ttattgaatt taatggagat
    28501 gtagatagat tttcaaaagc ttttacaaga gaagctttta gtttatcctt tttattggat
    28561 tcaaaaggtg caatagataa aaactctgtt ttaaataata aagattattc catatcaaca
    28621 ttagcattga atcaacaaat tatgatttta gagaaagtgg aagagttgtt agaaggaagt
    28681 atgtttttga atggtttaag tggagaagac aagataaaag gtctatttac agtacttcac
    28741 atggataaga aatttgttca tatgatgaac aatttcgatt actcacttat aaacccaaaa
    28801 ttgattatat acatgtacaa gagtattgta tttgataaag aaatagtttt tcttatgctt
    28861 ttacttagta agattggatt tgatattatc atattatgtc caggaggaga aaacaatata
    28921 gagaatgtta taaataacca attgattgat gtacacagat tggataaaat ggtatatgac
    28981 cttaaattaa attcgcttga aaatgatata cctttactta aaaaaatttt tggaaagagg
    29041 agacgatttt agatggggat aaatataaat agtttttcaa aagataagaa ctctgtagag
    29101 gttactgaga atttgcctac agcagttcaa gaagaaaact tcgatataat ggaatataca
    29161 aataataaaa aaaatgaact gagaaagtct aaggaagttg aggcattgac atctcttata
    29221 gaagtagaaa atccagatac gattttacag tttggtagga aagcatctga aggagtagca
    29281 agagtttctg atagcctttt aaatacaata aaattaaata gaaatgaaga aaattctaaa
    29341 atgctggttc atttaactaa aataatggac aagtttgatt tagacgattt tcaagaaact
    29401 aaggaaccaa atttcgttca aaaacttttc aaaaaagcta ataatgccat tgaaatgatg
    29461 tttcaaaagt atgaaacatt gggaggagaa gttgagaaaa ttcaaattga gcttgaacaa
    29521 tacgaaagag atatagcact ttctaataag caaataggtg ctatgttaaa tgaaaacttt
    29581 gagttttaca atgaacttca aaaatatatt gttgctggag aaatggcaat agaagaaatg
    29641 gataatgaaa tactaccttt ctttaaacaa aaatcagaga ctagtggaga ccaaatggat
    29701 gttgtcaatt atcaagaact attaaaagtt tatgatatgt taaatcaaag agtctatgat
    29761 ttaaggatag cagaaaatat agctatccaa acaataccta tgcttagagg tatgcaacat
    29821 aataattatg gtttaattag aaaaataaat agtgcatttg tcgtaacttt accagtattt
    29881 aaacaatgtt tatctcaagc tatattactt aaaaagcaag aattacaagc aaaatctcta
    29941 aaagcacttg atgacaaaac taatgaatta ttgcttagaa atgcacaaaa tgtaagtact
    30001 caaagtgcac aaatagctag aatggctgga acaagttctg ttcaaataga aacattggaa
    30061 aaaatgtaca atactattaa atctggtata gatgaaacta tgagaataga agaaaacaat
    30121 agagctttaa ttaaggataa tacaaagaga ttggaagaat taaatacgac tataatttat
    30181 aataagtagt attatattaa gggctaattt gtataaagtt ggccctttct atgtatatga
    30241 ggaggctaga cattgaaata tttcgattat atctgcaaag atgatttgga gagaattttt
    30301 ttgaaggagc cagaagattt tagtgctaag actgaaaaag atgtgttgaa atatgcactg
    30361 ggcgcttttt tatacgttcc agctactcaa tataatatga tttataagag tattataggg
    30421 gatgttaagg gagttagacc attagctatt tgtttagaag atgctgttgg agtaaatgga
    30481 gagttagagg ctatagaaaa tcttagatta attttaaaaa atattagcaa tgaaagtata
    30541 actaataaag atggaatacc actaattttt gtaagaataa aagatgtgga gcaactgttg
    30601 agaattaaag agattataat taaaaataga cattctataa caggaatatt aattcctaaa
    30661 gcaaattcgg agttaataga aaattgtata gaagctttag attctatgaa tcttcaagat
    30721 atgtatgtta ttccgataat agagacaaaa gaatttatat ataatgaaaa aaaagaactt
    30781 agttttacta atttgtacaa tgctatttta agacataagt caagaatatt aagcattaga
    30841 gtaggcataa ctgatatatt agggatgtat ggtataagga gagataagaa cttctctata
    30901 tataataatt tgatttgttc ttcatttata ttagatataa tcacatatct tcaaagacca
    30961 gagttagata tacctataag tggtggagta tcagaatttt ttgatatgac gaataaggat
    31021 ataagaaata agtatataga agaaatatta ttggataaat atcatggatt aattggaaag
    31081 accgtaattc atccaatgca aattcaaatt gtacaagcgc tatctacagt atcatatgaa
    31141 gattttactg atgctttgga tatactggat agtacagaca gtaagtatgg tgtgagcaag
    31201 ggtgtattag gagagagaat gaatgaaaca aatccacatt ttttatgggc taaaaaaaca
    31261 ttgattctat ctaaaatata tggtgtatta aataaaggag tagactatga agaattgctt
    31321 aaattttaat tgtgagattg acattattga taatccattg aatttagaac taaatgattt
    31381 tttagacgta ggaataagaa ataatagcaa aagacgtttt ttgtttatct caaagaaact
    31441 cggtaaacat ttagcatgta agccaagtga gatggataat ttgggaaaat taatggctac
    31501 aatttataat gaaaaaaata aggaagaatc aaatggtact gttatagctt ttgctgaaac
    31561 tggtacagct gttgggcata gtttttttga ttacttatgt ggagattatg aatttattca
    31621 tactactaga gagcagtttg acgagttaga aaatctggaa tttttagagg aacattccca
    31681 tgcaacagac cagaatttat actttggtat gcttaacaac tttaaatttg gagatgagat
    31741 aatattggtt gatgatgaga ttaccacagc caatacatgc atgaatataa ttaggaaaat
    31801 tcaaagcatt tatcctaaaa agaaatacac tatatgctca atactcaatt gggtaagtga
    31861 tgaaaattta agtaaagtta ctcgtttaga aagagagcta gattgtaaga tagattttgt
    31921 atacttattt aaaggaaact ttaaatttta tatagatgaa gatattattt caaattacaa
    31981 cgataattat agtattacta aattagagac tatttcaagt gataaagtaa atttagtagt
    32041 aaatcatatt tatgttgatt tagaagatta tattggaaat aagaaatatg taaaatatac
    32101 aggaagattt ggaattaata gaagagaaca atttagatta aaagaaatcg ttaagagaga
    32161 aagtaaaaaa ataactatgg ccagtaaaga atctttaaaa aatgaaaaag aaaagattct
    32221 atttttaggt actgaagaat ttatgtatat acctatgctg tttgcaaaag aaaaagaaaa
    32281 tcaatatgat atatactatc attcaacaac aagaagtcca attattaata ttgataagaa
    32341 taattatcct attaaaagta aatttatgct aaagagtttt tataatatag atattcaaaa
    32401 ttatatttac aatatagata aacataattt ttctaagtgt tatttatttg tagagctgaa
    32461 taaaagtgaa aattcttatc tagaatttat tgatataatt aaaaaaacaa gtataaaaga
    32521 attaactata gtatgttgta gctaaagtgt tttaacagga taaaatatat cacgatttta
    32581 agataggagt tgagtcatat gagagatttt agcacgtatt ctaaagatga tgttattttt
    32641 ttattaaaag atatatcaaa tttaattgaa gaagaaggaa ataaagaaag agaattaaaa
    32701 atacaaaatg gtagacatta ttcagaaatg ataccaatag agtatgaagt tagtgaagat
    32761 tatctgaatt tatatcatga gaaactaaga gaaaataaag ataaacttgc attttctata
    32821 ggagttatgt gtgaaaaaat aattaaaaaa aatggaaatg atgtagtact agtttcactt
    32881 gctagagcag gaacacctat tggtattttg gcaaaaagat atataagaag caagtataat
    32941 gtaagtttac ctcactatac aatctctata ataagagata agggaataga tatgaatgca
    33001 attgaatata tagtaaagaa tcatccgagc tcaaaaattc aatttttaga tggatggact
    33061 ggtaaaggaa ctatatcaaa agaacttgaa aaagcatgtg atgaacttaa tgttaaattt
    33121 aaaaaatatt ttgactcaac acttgctgta ttagctgacc ctgctggata ttcaggactt
    33181 tatggaatta gagaagattt cttaatacca agtgcatgtt taaattcaac agtttctggt
    33241 ttagtaagta gaactgtact aagagaggat ttaattagta aaaatgactt tcatggagct
    33301 aagttttata gacatttaaa agataaagat gaatctatga actatatcga aactatagaa
    33361 gaatgtttta aaaatcaatt taagaatata tctgatgaag ttgaaaactg ggaaagtgac
    33421 attataacaa gagatggata ctttgatgtt ttaaatataa aagaaaagta taacataaca
    33481 gatattaact ttatcaaacc aggtgtaggg gaaacgacta gggttttact tagaagagtt
    33541 ccgtataaga ttcttgttaa agacttaaat gataaatctt tagaccatat attcatatta
    33601 gctaaggaga aaaatgttga agttgaacaa atggatttaa aagcctataa atgttgtgga
    33661 ataataaaaa atatgaaaga tatttaggaa atatattaga caggagaata ttgaatgatt
    33721 gttttttcag atttagacag gagtatcatt tactccaata aatttttaaa tgctgatagt
    33781 aaatatgcta atatagaaat ttatagggaa aaagaaatat cttatatatc tttagatact
    33841 ataaatttaa taaagcaaat acaatattat ggtatgttta tacctacaac tactagaaca
    33901 gtggagcaat ttaagcgtat agaatttaat aaatatggga tttattttcc ttggagtata
    33961 acttcaaatg gaggagttat acttaaagat aatgaaatat tgaaatcatg gagtgaaaaa
    34021 atagataaac taaaatctga ttatgagccc attgaaagta tgattcataa atttaaagac
    34081 tatttgaatg tagatggaat aactaatttt aaagttgcag aagatacatt tttttatata
    34141 gttgttgatt tatcaagatt taatttggat tcaatcaaag aatatacaaa tatattggaa
    34201 tcaaaaaatt ggaagtttta tgtgagtggc agaaaaatat attttatacc aaaagaaatt
    34261 tcaaaagaaa atgctataaa atacttgact aaagaattag gaatagaata tttttatgca
    34321 gttggagatt ctattatgga ctatggtatg cttaatatct caaataaatc ctatgtattg
    34381 aagcatggtg atataaataa aaatgaaata gaaaattcat ttatatcaag ttctttcaat
    34441 ggaatgagtg gaactgaaga gatactatct aacattttag acgagaactg cctacttaat
    34501 atataatgac tgatatataa ataataaact agaaaaatct gaaataaatt atagtaaagt
    34561 ttatcatact aatttatttc agatttttat caatttatat ttaagtaaga actcaatatg
    34621 ttaaaatgaa ggtggagtca ttgcccagac tgagatacat acctcatcat cataattttc
    34681 atacacatgt ggtatagtac tgttaaaata aaaactatct ccttcctcca atagatattc
    34741 cttatttcca agtgttatct tcattttacc ttttattata tagccacatt cttctccttc
    34801 gtgtgtcaca aattcactgt ttttgtgtgt atgaccatct agtgtaacaa gtataaattc
    34861 tatttttttc ccagataaat taggaacaag aagttcgtaa aattttgtag aatcattagt
    34921 tactattttt tttcgattat ttttctttac tactatatca gagtcttctc ttccaatctc
    34981 ttcaaaaaaa taacctatat ttatatcaag agcttttgat attttccaca ttgctacaat
    35041 agatggaaca accatattac gctcaatttg acttattaag cctgtactta aatctgaatt
    35101 ttttgctaaa gtagagatac ttatattttt agattttcta agagatctaa ttttgcttcc
    35161 aacatctata ttcattagat acaaaccccc tcagacatga ttataacata taatttataa
    35221 agattaataa aataaaaaaa aagttttaca atacaagtat tttttatgct atattgaatt
    35281 ttctgaaaag atgcattata atagtattaa tattcaatat attgaatatt gataacgata
    35341 ttttaattta atgaaatgga ggtaattaag atgaagagtt ttgtaagtcc tgataatatt
    35401 tgggtattat gggcagttgt aactggatgg gcagcattta gtatttattt ggaacaaaag
    35461 tacaattggg catcaaaagt gtcgggagca ataatagctc ttgtaggagc aatgttatta
    35521 tcaaatctaa atattatacc agtagagtct gttgtatatg accaagtatg gggatatgtt
    35581 gttcctttag ccatagcttt attgttatat caatgtaaca ttaaaaagat atggaaagaa
    35641 agtggaagat tgctcattat atttcttgta ggttctgtag gaactgtact tggagctatg
    35701 attggatttt tagcattaaa aaatgtagta ccagatttaa atattgttgc agctatgatg
    35761 acaggttctt atataggtgg aaatgtaaac tttgctgcta tgtcaggggc gtttgatgcg
    35821 ccaggagaat tagtatcagc aacagttgta gcagataatc ttttaatggc attatatttc
    35881 tttgtcttaa tagctatacc atcaattgga ttttttagaa aacattttaa acatccacat
    35941 gtagatgaaa tggaaagtat aggtataact gaaggagaaa cagtagcagc aaattattgg
    36001 ggaagaaaag aaatatcttt aaaagatata gcaatagcca ttggtagcgc ttttataatt
    36061 gtcgcagtat ctgtagaact atcaacttgg tttaaagcta taataccatt atcaaatcct
    36121 tttttagcaa tgttaaatac tttatttgga aatcaatatc taataataac tactttgact
    36181 atgttgtttg ccacatttat gcctaatttc tttggtgata taaaaggagc acaggaattg
    36241 ggtacatttt taatatatat attctttgtt gtagtaggtg ttcctgcttc aataacatta
    36301 ataattcaaa aatcaccatt actactttta tactgcggaa taatggtact aataaacatg
    36361 ctagttacat ttatagttgc aaaaatattt aagtttagtt tagaagaagc tattcttgca
    36421 tctaatgcaa atattggtgg tcctacaaca gcagcagcta tggcaatatc aaaaggatgg
    36481 tctaagctag tagggccaat acttatagtt ggaacatttg gatacatagt cggtaattat
    36541 tttggtcttt tagtaggaaa tattttgata taattttacg tgaaaaaaag ttgtttttta
    36601 gaaagatttt tatttaattg gaggttgtta tgattttcga tggacattca gatacttggg
    36661 cagatgtaac aataagaagg cttaacgggg aaaaagatgt atttagaaga atccatcttg
    36721 ataattttaa aagagcaaat atagaaggag ctatttttgt aatatggata gacccaccat
    36781 atgataaaga gcctgaaaaa agagttaaac aaattataag ctctataaaa gatgaaattg
    36841 aagatatgaa agatgttgta aacatagtaa aaaaatattc agatatagat aaaggtataa
    36901 gtgaaaacaa aataaatatt ttaattggtc ttgaaggatt aagccatatt ggaagaaata
    36961 tagatttact agattatttt tataaagaag ttggtgcaag acatgcatca ttaacttgga
    37021 atgaacagaa tgaacttgct acaggatgtt taggggatga aaatcgtggt ttaactgaaa
    37081 taggtaaaaa agcagtcaat aaaatggaaa aattgggcat gatagtagat gtgtcacatt
    37141 taaatgaaaa atcattttgg gatttaatgt ctgttgcaag taagccagtt atagcttctc
    37201 attctaatgc taggagttta tgtgatgcta aaagaaatct tacagataat caattaaaag
    37261 aaatttcaaa tacaggtgga ttagttggaa taaatagttt tagaggattt actaatagta
    37321 atcctaagaa gcaagattta atacatttgg ttgaccatat agactatatg gtaaatctta
    37381 taggtataga acatgtatct tttggttttg attttagtga ttacttaggg aaagagacta
    37441 taggttcttt tactggaaat gctagtgtgc caagtgtaga tggtcttgaa agcacacata
    37501 cagcacagaa catagtagta gagcttgaaa atagaggtta taaaaagaaa gatatagagt
    37561 taatctctag tggtaatttt tataggctct ttaaagatat actaaaataa tataataaag
    37621 aacttaaatt tataaaatag tatatttata caaataatat ctacatattt aaattggcgt
    37681 tcataattta ttaaattaaa ttttaatgtt actagaagtt tcaattctag taacattttt
    37741 tatgttaaaa acagatatag atatatattg acaatgataa ttattattga taaactataa
    37801 aagtgaaaat gataataatt ttcattttaa ttaataaaag atttttaagg agggagaatg
    37861 aagaaaaggt atttagtcat aggaataata attttgtctt atttttcttt atttatagga
    37921 gctgaagata taaatataat gcatatattt gccaaaaatc agcataaact tatgatattt
    37981 atcatgagta gaataccaag attaataagt atattgattg ctggtgtagg aatgagcata
    38041 gcaggtctta taatgcaaca gataagtaag aataaatttg tatcacctac aacaggtgct
    38101 acaatagatg cagcacaatt tgggatagta atatgtatgc ttttagtacc aactgcctct
    38161 atttttacaa agacaataat agcatttgta ttttctcttg tagggacttt tacgtttatg
    38221 aaaataatag ggaagttaca atttaaaaat ataatctttg taccattagt aggtataatg
    38281 tttggaaata taataggttc tatgacggat tttatcgcgt ataaatacga tttgagtcaa
    38341 aatgttagtt cttggatgca aggtgacttt tctatgatac ttaaaggaaa ttatgagata
    38401 ttatatatca caatacccct tataatatta gcatatattt atgcaaataa atttactgtt
    38461 gttggtatgg gtatggattt tgcgacaaac ttaggtctta gttacaaaag aattgtcaat
    38521 ataggtctga taatagtagc acttgttaca gtatgtgtag tcgttacagc tggtaatatc
    38581 ccttttatag gtctaatagt tcccaatatc gtatccttgt atatgggtga taatataaga
    38641 gcaagcatat ggtatactgg tttacttgga gcgatatttg tacttatatg tgatattttt
    38701 ggaagaatta ttatttatcc atatgaaata tctatagggc ttacagttgg agttatagga
    38761 agtatactat tcctttattt aatattaagg aggaatgtaa atgaagctta gtctcaatca
    38821 aaaaatttac tttgtaagta tattactatt catatcaata gcacttttta taatttatgg
    38881 aatagacatg aatcatttag aatatgcatt atcacaaaga ataccaaaaa tatttgctat
    38941 ggtattaggt ggaggatgta tagcatttac tactgtagtg tttcaaacaa ttacaaacaa
    39001 tcaaatatta acaccaagtg tactaggttt agattcattg tatgtaatga ttcaaactat
    39061 aatagtattc atatttggtt caagcagtag tctgattata aatgaaaatt ataattttat
    39121 tattaatgta gctatgatga ttggagcttc attgttatta tataagacgt tatttgaaaa
    39181 aaataaaaat aatattttct ttttaatcct agtaggaatg atatttggaa ccttatttaa
    39241 aagtgcaaca acatttattc aggtaatgat agaccctaat gaatttttag ctttacaaac
    39301 tagtataatg gcaagtttaa acaatataaa cactaatgta ttgttaatag catttataat
    39361 aattattgcc ataatacctt ttatatatga tgaaataaag tatttagatg tattgtcgtt
    39421 aggtaaagaa caagcaatta atttaggtgt agattttgat aaagcagtta aaaaaatgat
    39481 aatattaata gcaattttag tctcaatatc aacagcactt attggaccta tgacattttt
    39541 aggactttta cttgctaata taacaagaga aatttttaaa acatataagc atacatactt
    39601 gatttcgggt tctatgttga tagggatgat tacattgata ataggtcaat tttttataca
    39661 acatgtattt aagtttgata caactttaag tgtagtaata aattttatag gaggaattta
    39721 ctttattcaa ttactattaa agggggcaaa tagatgatag agataaaaaa tatatttaaa
    39781 agatataaga ataaaaatgt tgtagatgat gtctctttta gtatagaaaa gggaaagata
    39841 acatctttta taggaccaaa tggagctgga aaaagtacag tactatcaat agttacaaga
    39901 cttataggag gtgatggtgg agaagtaata atagaaggaa aaagcttgac taattatagc
    39961 aataaagaat tagcaaaaaa aatagctatt cttaaacaat ctaacaatat aactttaaag
    40021 cttacaataa gagaattagt tggatttgga agatttccat atagtgaagg taatctaact
    40081 aaagaggatg aaaattatat tgatgaagca atagaatata tgaagttgac tgatatacaa
    40141 cataagtatt tagatgaatt gagtggaggt caaagacaaa gagcttatat agcaatggta
    40201 atagctcaag atacagagta catattactt gatgaaccac tgaacaattt ggatatgaac
    40261 cattccgtac agatgatgaa ggtacttaga agtctttgtg atgagttaga taagactata
    40321 gtattagtaa tgcatgatat aaattttgca tcatgttatt cagataatat agttgcatta
    40381 aaaaatggaa aagttaaaaa agtaggtcgt acagatgaga tagtaaatga aaaagtttta
    40441 gaagatattt atgagatgaa ttttaacatt aagaatataa atggaaatcg aatctgtatt
    40501 tatttttaaa atattgtata tgaaaataaa cttgctatat gattttttat aaagttttta
    40561 aattaaaata aagattaata ggagagagga aaatattgat atgaataaaa aagcagcaat
    40621 agtagcagca gtagctatta taggattagt gacagtgttt gcattaggtg gaagtaaaaa
    40681 gaatgaatca aaaacatcag aaaattcaaa taatactatg aagataactc ataacttggg
    40741 agaaacagat gtaaaattaa atcctaaaaa agtagtggta tttgattatt cggctttaga
    40801 tacaatggat gcattaggtg tagctgagaa tttagtagga cttccaaaag cgagtttacc
    40861 agcatcctta gaaaaatata aggatgacaa gtatgcagat ttaggaggat taaaagaacc
    40921 agatttagag ggtataaagt cggcaaatcc agatttaatt ataataaatg gaagacaaga
    40981 agatttttat gagcaattgt caaaaatagc accaacaata agtacaagta aagatgataa
    41041 gaagtactta gaatctgtta aaaataatat agacaaaata gcaaaaatat ttggagtaga
    41101 agaaaaagca aatcaagaat ttagtaaaat tgaaaagaaa atagaaactt tgaataaaaa
    41161 agtaacagat aaaaatttaa atgctttaac tataatggta aatgaaggta atttaagtgt
    41221 atttggtgaa gaatcaagat ttagtatatt atacaatagt tttggatttg aaaataaaga
    41281 taaaaatata aaagaatcaa gtcatggaca aaatattact tttgaatata tagctaaaca
    41341 aaatccagaa gttatgttcg ttatagatag aggaatagct actggcagtg atgtaaaaga
    41401 aagctcaact gcaaaatctg ttttgaacaa tgatataata aaatctatgg atgcatataa
    41461 gaatgataat ataatatatt tagattcacc aacatggtat gtaaatgatg gtggtctaac
    41521 atctttaaat aaaatgatag atgatgcttc aaaagcggta aattaataat ccgatataat
    41581 caaaatatat tttttataaa atgattatca aataaactag gagtttttaa gaactcctag
    41641 tttatttttt gcataaaaaa gatatagttt aaaca
//
LOCUS       DAMJWS010000027        40590 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMJWS010000027 DAMJWS010000000
VERSION     DAMJWS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.027"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(172..741)
                     /gene="yfcE"
                     /locus_tag="PC399_001511"
     CDS             complement(172..741)
                     /gene="yfcE"
                     /locus_tag="PC399_001511"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF2592380.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(766..1566)
                     /locus_tag="PC399_001512"
     CDS             complement(766..1566)
                     /locus_tag="PC399_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF2592381.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            1701..2561
                     /locus_tag="PC399_001513"
     CDS             1701..2561
                     /locus_tag="PC399_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592382.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            2561..3247
                     /locus_tag="PC399_001514"
     CDS             2561..3247
                     /locus_tag="PC399_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2592383.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            3299..3841
                     /locus_tag="PC399_001515"
     CDS             3299..3841
                     /locus_tag="PC399_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592384.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            3920..4387
                     /locus_tag="PC399_001516"
     CDS             3920..4387
                     /locus_tag="PC399_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF2592385.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            5096..6232
                     /locus_tag="PC399_001517"
     CDS             5096..6232
                     /locus_tag="PC399_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF2592386.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            6291..7073
                     /locus_tag="PC399_001518"
     CDS             6291..7073
                     /locus_tag="PC399_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2592387.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            7092..8102
                     /locus_tag="PC399_001519"
     CDS             7092..8102
                     /locus_tag="PC399_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2592388.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            8121..8918
                     /locus_tag="PC399_001520"
     CDS             8121..8918
                     /locus_tag="PC399_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2592389.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            9016..9861
                     /locus_tag="PC399_001521"
     CDS             9016..9861
                     /locus_tag="PC399_001521"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF2592390.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            9948..11123
                     /locus_tag="PC399_001522"
     CDS             9948..11123
                     /locus_tag="PC399_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2592391.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            11319..12221
                     /locus_tag="PC399_001523"
     CDS             11319..12221
                     /locus_tag="PC399_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2592392.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEDLINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            12235..13239
                     /locus_tag="PC399_001524"
     CDS             12235..13239
                     /locus_tag="PC399_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2592393.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(13383..13610)
                     /gene="acpP"
                     /locus_tag="PC399_001525"
     CDS             complement(13383..13610)
                     /gene="acpP"
                     /locus_tag="PC399_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2592394.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            13782..14207
                     /locus_tag="PC399_001526"
     CDS             13782..14207
                     /locus_tag="PC399_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592395.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            14255..14461
                     /locus_tag="PC399_001527"
     CDS             14255..14461
                     /locus_tag="PC399_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592396.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            14643..14822
                     /locus_tag="PC399_001528"
     CDS             14643..14822
                     /locus_tag="PC399_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592397.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            14856..15056
                     /locus_tag="PC399_001529"
     CDS             14856..15056
                     /locus_tag="PC399_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592398.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            15309..16325
                     /gene="ccpA"
                     /locus_tag="PC399_001530"
     CDS             15309..16325
                     /gene="ccpA"
                     /locus_tag="PC399_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF2592399.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(16448..16846)
                     /locus_tag="PC399_001531"
     CDS             complement(16448..16846)
                     /locus_tag="PC399_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592400.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            17058..17963
                     /locus_tag="PC399_001532"
     CDS             17058..17963
                     /locus_tag="PC399_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592401.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            18078..18258
                     /gene="ssrS"
                     /locus_tag="PC399_001533"
     ncRNA           18078..18258
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC399_001533"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            18441..19658
                     /gene="cdeC"
                     /locus_tag="PC399_001534"
     CDS             18441..19658
                     /gene="cdeC"
                     /locus_tag="PC399_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF2592402.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(19758..21080)
                     /locus_tag="PC399_001535"
     CDS             complement(19758..21080)
                     /locus_tag="PC399_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2592403.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(21131..21925)
                     /locus_tag="PC399_001536"
     CDS             complement(21131..21925)
                     /locus_tag="PC399_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2592404.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            22035..23480
                     /locus_tag="PC399_001537"
     CDS             22035..23480
                     /locus_tag="PC399_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2592405.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            23509..23718
                     /locus_tag="PC399_001538"
     CDS             23509..23718
                     /locus_tag="PC399_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF2592406.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            23738..25132
                     /locus_tag="PC399_001539"
     CDS             23738..25132
                     /locus_tag="PC399_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2592407.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            25372..25557
                     /locus_tag="PC399_001540"
                     /pseudo
     CDS             25372..25557
                     /locus_tag="PC399_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(25666..26832)
                     /locus_tag="PC399_001541"
     CDS             complement(25666..26832)
                     /locus_tag="PC399_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2592408.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVERVGGNTKKEAEKSLR
                     DAINEYENAGIVFDETNMSLSDYLNFWYKEYVLLNCKYNTQESYRNLIENHIEPRLGK
                     CKLKSINPAIIQEFLNNKSKETYTQNGEEKHYTKGVLKAIYVVLNAALKSAVYPYKLI
                     KENPVQYASIPKNVLKVKNESDNKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELASPKTKTSIRDIKIGDTLSRILKE
                     EKLNQKKQKIKIGKWYKETEYDWVCRKKDGSFVNHNNIDAAIRTINKKLNINFNFHCL
                     RHTHATLLLENGANVKYIQQRLGHSQLSTTMDTYSHVTSKMESETIDILEGILQ"
     gene            complement(26970..27176)
                     /locus_tag="PC399_001542"
     CDS             complement(26970..27176)
                     /locus_tag="PC399_001542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592409.1"
                     /translation="MNLSFNKKKKFELSKEELELIENWLGNKYAGDMTIPAIVDFSLE
                     TDIEYEKIVVYLAEKVLESRDKKH"
     gene            complement(27235..27456)
                     /locus_tag="PC399_001543"
     CDS             complement(27235..27456)
                     /locus_tag="PC399_001543"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592410.1"
                     /translation="MLKELRKKKKLTQIELAKRVGCHRSQISRLENNENKDLTIPALI
                     ELEIALGLEEKYLVNYFADEYIKKRKLHK"
     gene            27740..27877
                     /locus_tag="PC399_001544"
     CDS             27740..27877
                     /locus_tag="PC399_001544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592411.1"
                     /translation="MNKTKYYDILKLNLLMKKLKELDKNKINEYKIKVKEIHKINKKE
                     E"
     gene            complement(27914..28309)
                     /locus_tag="PC399_001545"
     CDS             complement(27914..28309)
                     /locus_tag="PC399_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592412.1"
                     /translation="MNRIKELREEKGISLDKLSEDLHINKSTLSRIENGLREPKKSTI
                     EEYANYFDVSTDYLLGRTDVRNSLFINKNEKDYDAENFKTEKELIENMYLDEDMKEVF
                     NIFSELSPDAREKALKVAELFLLDEKNKK"
     gene            28488..28751
                     /locus_tag="PC399_001546"
     CDS             28488..28751
                     /locus_tag="PC399_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2592413.1"
                     /translation="MYLNRLEGLMKENRHTQKNVADILGLSSYGFRLKLKGKNEFKAS
                     EIKKISKLYNVSADYFFSDEVAKIAIKEERGNNNEESNHNQAK"
     gene            28720..29508
                     /locus_tag="PC399_001547"
     CDS             28720..29508
                     /locus_tag="PC399_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDF2592414.1"
                     /translation="MKNLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLRSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MEQELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADSVA
                     SSDNSILVGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLGVI
                     ETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKSSKQLSMLS"
     gene            29563..29700
                     /locus_tag="PC399_001548"
     CDS             29563..29700
                     /locus_tag="PC399_001548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HDF2592415.1"
                     /translation="MKHEQTTLRIPEDLYKALIDLSKEIGMPIASIVIIACWLYISKI
                     N"
     gene            complement(29686..29997)
                     /locus_tag="PC399_001549"
     CDS             complement(29686..29997)
                     /locus_tag="PC399_001549"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592416.1"
                     /translation="MTTITVRIYTPLNENLEKISYQTGILKSSLILYAINDIIRNSKV
                     NELQSISYKSDDTVRSTLRIPGVLKELLEKTAKENNLSVNSLINNAVHSFCISHWLIY
                     L"
     gene            30118..30288
                     /locus_tag="PC399_001550"
     CDS             30118..30288
                     /locus_tag="PC399_001550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592417.1"
                     /translation="MEKVRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            30291..31082
                     /locus_tag="PC399_001551"
     CDS             30291..31082
                     /locus_tag="PC399_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDF2592418.1"
                     /translation="MNNLQIFEKLEFGQIRMVEVDKKPYFVATDIAKCLGYANTSKAI
                     NDHCRWVTKSYIPHPQNENKILEVNTIPESDMYRLIVNSKLPNAEKFESWVFDEVLPT
                     IRKTGGYIHTTEDMTDDEIMARALQVAQKTIEKKSREIEEKDKVIQLQQPKVLFADAV
                     ASSDDSILVGELAKLLKQNGIDTGEKRLFAWLRDNGYLIKRKGEDYNTPTQKSVDLGV
                     IEIKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKKNNQLSMLS"
     gene            31139..31276
                     /locus_tag="PC399_001552"
     CDS             31139..31276
                     /locus_tag="PC399_001552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592419.1"
                     /translation="MSNKKKYTLSITEELYNKCKEKANQKGMSVNEYILSVISKNLKN
                     I"
     gene            complement(31288..31455)
                     /locus_tag="PC399_001553"
     CDS             complement(31288..31455)
                     /locus_tag="PC399_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDF2592420.1"
                     /translation="MPSKLPRYTLRIDNNLLEKIKYIAESEGRSANKEIEQLIKKHVE
                     SYEKINGKINV"
     gene            31608..31754
                     /locus_tag="PC399_001554"
     CDS             31608..31754
                     /locus_tag="PC399_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592421.1"
                     /translation="MPDELNEELHRQSQRKGLSKNAFIINILWKEFEDLQDLKDEQEV
                     DKYE"
     gene            31747..32061
                     /locus_tag="PC399_001555"
                     /pseudo
     CDS             31747..32061
                     /locus_tag="PC399_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212439.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32326..32599
                     /locus_tag="PC399_001556"
                     /pseudo
     CDS             32326..32599
                     /locus_tag="PC399_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056356.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32589..32741)
                     /locus_tag="PC399_001557"
     CDS             complement(32589..32741)
                     /locus_tag="PC399_001557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592422.1"
                     /translation="MVIPIAKNTKQTSKTVASKASKILKDGRYSAASKSVAGSALSQT
                     KKGPKK"
     gene            32800..32997
                     /locus_tag="PC399_001558"
     CDS             32800..32997
                     /locus_tag="PC399_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592423.1"
                     /translation="MAINDNINKILKDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKIADYLDITLDELVGR"
     gene            33036..33470
                     /locus_tag="PC399_001559"
     CDS             33036..33470
                     /locus_tag="PC399_001559"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2592424.1"
                     /translation="MGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNNYKN
                     KISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKNGLL
                     KSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKELREGVI"
     gene            33598..33786
                     /locus_tag="PC399_001560"
     CDS             33598..33786
                     /locus_tag="PC399_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592425.1"
                     /translation="MKIIYKNKVYKVEQDKKLFRITYYDEQRGSKKFNKEKKIKRSVL
                     TRDIELVNLYLPAHLKIK"
     gene            complement(33921..34091)
                     /locus_tag="PC399_001561"
     CDS             complement(33921..34091)
                     /locus_tag="PC399_001561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592426.1"
                     /translation="MSNKDIYTREEDKRFTLRINKLLFEKIEQLAQKDKRSIGREIEF
                     ILEKYFEDNPLE"
     gene            34234..34392
                     /locus_tag="PC399_001562"
     CDS             34234..34392
                     /locus_tag="PC399_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDF2592427.1"
                     /translation="MTNERVRFTFRLPAPLLEKIKNRASIEGSSMNSLILHILWDYIK
                     EIENKEVK"
     gene            34392..34619
                     /locus_tag="PC399_001563"
     CDS             34392..34619
                     /locus_tag="PC399_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592428.1"
                     /translation="MVELVKEFDLQTIKVGNAVKVNCKRFGFEIDCIVVVATEKELNL
                     AYFDEGRGCMEYQALITEDIQDGDYEIKILS"
     gene            34717..35031
                     /locus_tag="PC399_001564"
     CDS             34717..35031
                     /locus_tag="PC399_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592429.1"
                     /translation="MESLKRIKKMVQKQLVLAELEINKNSKLYEELENKDRGLIDDIH
                     MREYLREKVAWERVKYAIENILGGINLEIKSKEHEESEDYKIFQLILEELERDKPIDV
                     QI"
     gene            35115..35624
                     /locus_tag="PC399_001565"
     CDS             35115..35624
                     /locus_tag="PC399_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDF2592430.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNETEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNIIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            35634..36242
                     /locus_tag="PC399_001566"
     CDS             35634..36242
                     /locus_tag="PC399_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF2592431.1"
                     /translation="METNNVYIKLVNIQSTLKAPKSQFNSFGKYNYRSCEDILEGLKP
                     ILKEEKALVILDDNIVQIGNRFYVEATATLIDAETGEKVSTKALAREDETKKGMDLAQ
                     VTGSVSSYARKYALNGLFCIDDTKDSDATNKHGNEQKKKEVNESELNTLYSLGESIEK
                     DKNRVDSEVYKKFGKLAVDLTKQEYEKVLNGYKSILEKQKQE"
     gene            36252..37133
                     /locus_tag="PC399_001567"
     CDS             36252..37133
                     /locus_tag="PC399_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2592432.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLCKRSKNGRDATANILKELIENGYITRKPCRDSNTNKMLGGYDYEVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAVDEAVGSGVKNYKYVKTILNN
                     WIEAGVKTVLELTEYQNEFERKKKSKQEKKQSNSKTVNTHNVNKNKFANFNQTFTQYE
                     EKELDEIIKKSQKEKFK"
     gene            37194..37367
                     /locus_tag="PC399_001568"
     CDS             37194..37367
                     /locus_tag="PC399_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDF2592433.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            37383..37790
                     /locus_tag="PC399_001569"
     CDS             37383..37790
                     /locus_tag="PC399_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2592434.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            37864..38058
                     /locus_tag="PC399_001570"
                     /pseudo
     CDS             37864..38058
                     /locus_tag="PC399_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38110..38367
                     /locus_tag="PC399_001571"
     CDS             38110..38367
                     /locus_tag="PC399_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592435.1"
                     /translation="MIIIRSQDKTDLVKADWINVDKECVYAMFGDMDNFKKIGEYEDE
                     KRAIQVLDNIQRFIEGGTRTDSIDSYKVRSYRNRIFQMPVK"
     gene            38381..38746
                     /locus_tag="PC399_001572"
     CDS             38381..38746
                     /locus_tag="PC399_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592436.1"
                     /translation="MLKVEKYFSGSLGTNIIDDDPTCRNYLALYCCVHGVRKNGELIF
                     PTCEKMLIEFNVDKNRKKKKKGSKVKLINAKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIEEE"
     gene            38864..39199
                     /locus_tag="PC399_001573"
     CDS             38864..39199
                     /locus_tag="PC399_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592437.1"
                     /translation="MNILASVILVIGSFIAGRVYEYRLNLNENDEADSKVLLDVFNEI
                     SVLREENKSLKEKLQEKELLFINRLIDFLHDKKICECCIYDCKIDDIEYDCEDGIKKW
                     LDSEELIFE"
     gene            39307..40380
                     /locus_tag="PC399_001574"
     CDS             39307..40380
                     /locus_tag="PC399_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2592438.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSRKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGETMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
BASE COUNT        16125 a         4497 c         7141 g        12827 t
ORIGIN      
        1 taagttatat ttcaatatag catataataa aacaagtaaa ccagattcta tttaattaga
       61 agtttaaggt attgtactta aacttctaat tgagatagaa ataaaatggt ttacttgtct
      121 ttattatgaa tttaataaat taaagcttat tttattttta ttaaaaattt actaatcatt
      181 gatatttatt atatttccag tattgatgtc aattaagttt atatcaagtt caagctcatc
      241 ctcatcatca gtttttatga tatctataat tgcgacagaa tggcttccat cttttgggat
      301 agaagtactt cctggattta ttactattaa attatcatcc atgtatagtt tttttacatg
      361 agtatgtcct aaaactaata tgtcaccacc cattttcttt gctttgtcaa taatttcttc
      421 ttctgattct atataaccat gattcaatat aattctaatt tcaccaaatt ggctcatgac
      481 ataaggactc tgaataggat gttctataac catctggtca acatctgcat cacagttccc
      541 ttttacaatc acaatgtttt ctaatttatt taatgtctca ataaattttt taggattata
      601 tccttctgga atatcatttc taggtccatg atataagaca tctccaccat gtaataaaac
      661 atcacaatca gataatacat ttaacgcttt ttcaaagtaa agtaagctac catgagtatc
      721 actcattatt cctattttca tattaaaaat cctcctaagt acagtctata aaaatttttc
      781 agatactcta tgccaaaact catagtttga cattctaagt aattttattt ttgcatcaga
      841 tatactaact tttatatttt cgatttgtct aaatctgtgc tcaactccat caacgacaat
      901 caatacagaa tcttcaaatc tatattctgg tgcaatcttt ataacagatt catttgaaca
      961 tattatactt gatgtaaagc acctatatgc attggtattt atcggatgta aaggtgtaat
     1021 ctgcataagt tcaagattta tatcaacaat actacctcca gcagaatagt tataggctgt
     1081 tgaacctgtg gaagtagaaa taatcatacc atctccacta aaattttgta tatgtttatt
     1141 atcaaggctt aaatttaggt gtatagtcct agacttgtct cctcttataa caacctcatt
     1201 tacagcaagc atattagaac cactagtagt tgtatatact tccgcattaa gaagtgacat
     1261 ttcttgaata atataatctt tttttgtata agcttcaata aagctatcaa tcttatctgg
     1321 taaaatatca ggaaaaaacc ccagatgacc tgtatttatt cccattatgg gaatctcagg
     1381 aaaatcgaaa tctctaactg ttcttaaaaa agagccatca ccaccaattg atataataag
     1441 ctctgtgtct ggataaatat ctgaatacac ttcaaagcca gcattgataa gttttctagt
     1501 taagagttct ttagtctcaa gggacttatt taattggttt gtatttatag ttataattct
     1561 tttcatatat tcacctcttc atactttgta ttataacata gttttataat tcttaattta
     1621 taaatttata aatgaccttt gtagtgaatg tgttaaaata tgtataggtg ggtataaaaa
     1681 aatgcataag aggtgaagat gtgtttagca taaggaatat cacaaaaaaa ttaggaaaat
     1741 tcaagttaaa taatataaac ttagagttaa aggaagggga tattgttggg attataggac
     1801 caaatggttc gggaaaaacg actttaataa aaattataat ggggattatt gatgcagatg
     1861 aaggtgaaat tgaactttgt aatgaaacta ttgaaaattc tccaataagc tttaaaaaca
     1921 atattggttt tgtatatgat tctttacaat tttatccgca tttaaaggtt aaggaattta
     1981 gaaaaatagt ttctttattt tataagaatt ttgatagaga aaggtttgat gaatacctta
     2041 ataagtttga tatagaagaa aatatgcata ttgaaaattt atctaaaggt caatctgaaa
     2101 agcttatgtt atcaagtgca cttagtcaca atgctaagtt attgattttg gatgaaccaa
     2161 ctgctgggat agacccaatt gtaagaactg aaataatgca gtacttacaa gattttgtga
     2221 aaaatggaag tagtagtgta ataatttcta ctcataatac tgataattta attaaaattg
     2281 ctgattatct tgtttttata aatagaggaa atcagatttt tactgttaag aaagaattaa
     2341 tagaacaaga atataagata attagggcaa ataaagcaga gttagaagca attaaagaaa
     2401 gcatagttgg tgtgaaagag tataaatatt ataatgaggc acttgttaaa gttggagata
     2461 gcttgaaagt aaaaagccta ttgatagaaa ttgataagca taaggtcaaa aatcctacta
     2521 tagaagaact aatgtattat tatgtaaatg aggtgagatg atgttcaatt taataagaaa
     2581 agatttaata atagggatta gttcagatgg tatccgtaat ttaaagtaca tattgctatt
     2641 ttttgtgttt tattttttcc ttaattcaat ttcatattat acagtaagta ttgttgttag
     2701 ttatttgata tttataaata cgtttgagtg tgattatgaa aatgattcaa ggatttttat
     2761 aaggagtatg ccagttagta tagaagatat agtttactca aaatatttat taggtgtagg
     2821 attaataatt tctgtaacaa taattgtgtc cttactaagt aaattgacat cactagtgtt
     2881 ctttagaaat atggttttaa atgatgtatt tttttctgta aatatatttt tagcaatttt
     2941 gacgatttta ctgcctttaa tttttaagtt tggatatgga aaaatgaaag tttgtggttt
     3001 tatagtaagt attttaattt actttgtata tggaagtctt ttgaagatga ttagtatgat
     3061 tgtatatcaa gtaaagcatt ttaattatag taaagttgga ggagtatatc tttcaaatta
     3121 tgtaactgat atagctaata ctaagtatat aaatttatat agtatgacat ttttaacaat
     3181 aataattttt ataatatcaa tgtatttttc tattaagatt tcaaaaaaga ataagtttaa
     3241 ttattaaaat tttgattaat attatagcac aatagcacaa aatataagga gactaaaaat
     3301 gaagaagttt ataaaagttt cattttttat tttggggatt tttacactat tcatagctat
     3361 aaataaatta gtatatagag atatggtaag tgtcaaagat tatgaaataa atcatttaga
     3421 ggaaattgtt tttgattttg atagcaggag aagtagagat gttggtacta ttgtaagatt
     3481 taaaataagc aataatagta aatatagtta taaattaaac agtgctaaaa tgaagtttga
     3541 aaattgtgtg gaaaataaaa agggaatgaa taagtgttct gcatatgtaa atgtagattt
     3601 atttaatgaa aaaaattcta taactgaaag aactctgaaa tatggaataa aaccaaataa
     3661 tcaaggttat gttgattttg taattccaaa aggactaaat tttgattata ggtactttaa
     3721 tgaatctggt atgagtgtag agtataaagg agaatatatt gtcaatattc caatggcaaa
     3781 aggactttat ctagtagttg gaaaaaataa tgatacatgg aatgctaata tcatggaata
     3841 atttgaaaaa taaatcaatt atagatataa ttaagaataa tatgaaattt ataaaagttc
     3901 agactaggag ggatatttaa tgtcaataag taatgtaaga gagtatttta aacaatttgg
     3961 caaggaagac agtatattag aatttgaaca atctagtgct actgtggaac ttgcagctga
     4021 agcagctggg gttattccag caagaatagc taagacactc tcatttaaaa taggagatga
     4081 tgcaatttta attgtgacag ctggtgatgc taagattgat aataagaagt ataaggctga
     4141 atttaattgt aaggctaaaa tgttgacacc agaagaagtc ttggagttta ctggacatgc
     4201 tattggtgga gtctgtccat ttggtttaaa aaattctata aaagtctatt tagatgattc
     4261 aatgaaaaga tttgatacag tatttcctgc atgtggcagt agtaactctg caatagaact
     4321 tacatgtgaa gaaatggaga agttttctaa gtgtgaaaaa tgggtagatg tttgtaagaa
     4381 ttggtaaaaa aatatagtaa actatagtag gaattagtaa aaaactacct ctgtatacaa
     4441 tctatacaga ggtagttttt ttgtgtaaat atttataaat ctaagaggaa aatctaattt
     4501 aaactaataa tccaaaataa agttaaaaaa ttctgatttt ttttcgaaac aataaaaaaa
     4561 tgacaaaaaa gtggaaaaaa tgtaataaat attgttaaaa aaataattag aaattattga
     4621 aaaattatcc tttgagtgat actattatag atgtaagata tttaaaaata aaaaatagtc
     4681 agataaaaat acgattattt agctctatta aaataatttt tatacgataa aaatcttctg
     4741 tacatggcta aaaaacagat gtacagacaa tatttaatgt ttaatatacc ctaatataaa
     4801 tatcgtaaac aaattatttt atctgtaacc agtgtgaaag aaatcttatt ctaacttgaa
     4861 atttcacaaa attccaaaaa gcgagatttt ttttaagtat agttgatttt aaattaacaa
     4921 tttatgatta aattaatgtc ataattggtt agtataatca catataaata tagagcgtag
     4981 tttgattaag gttataaatt tctcgacgta tattcaaata ccttattata gatgttgccg
     5041 agattttaat taataaatga aaataaaata tttataaaat actgaggagg ctattatgga
     5101 tttaaattct aaaaaatatc agatgcttaa agagctatat gtaagcttcg ctgaaaatga
     5161 agttaaacct ttagcaacag aacttgatga agaagaaaga tttccttatg aaacagtgga
     5221 aaaaatggca aaagcaggaa tgatgggtat accatatcca aaagaatatg gtggagaagg
     5281 tggagacact gtaggatata taatggcagt tgaagaattg tctagagttt gtggtactac
     5341 aggagttata ttatcagctc atacatctct tggctcatgg cctatatatc aatatggtaa
     5401 tgaagaacaa aaacaaaaat tcttaagacc actagcaagt ggagaaaaat taggagcatt
     5461 tggtcttact gagcctaatg ctggtacaga tgcgtctggc caacaaacaa ctgctgtttt
     5521 agacggggat gaatacatac ttaatggctc aaaaatattt ataacaaacg caatagctgg
     5581 tgacatatat gtagtaatgg caatgactga taaatctaag gggaacaaag gaatatcagc
     5641 atttatagtt gaaaaaggaa ctcctgggtt tagctttgga gttaaagaaa agaaaatggg
     5701 tataagaggt tcagctacga gtgaattaat atttgaggat tgcagaatac ctaaagaaaa
     5761 tttacttgga aaagaaggtc aaggatttaa gatagcaatg tctactcttg atggtggtag
     5821 aattggtata gctgcacaag ctttaggttt agcacaaggt gctcttgatg aaactgttaa
     5881 atatgtaaaa gaaagagtac aatttggtag accattatca aaattccaaa atacacaatt
     5941 ccaattagct gatatggaag ttaaggtaca agcggctaga caccttgtat atcaagcagc
     6001 tataaataaa gacttaggaa aaccttatgg agtagaagca gcaatggcaa aattatttgc
     6061 agctgaaaca gctatggaag ttactacaaa agctgtacaa cttcatggag gatatggata
     6121 cactcgtgac tatccagtag aaagaatgat gagagatgct aagataactg aaatatatga
     6181 aggaactagt gaagttcaaa gaatggttat ttcaggaaaa ttattaaaat agataaatat
     6241 taaaatatac acaataggtc gatattaaaa tagatatgga ggaaggattt atgaatatag
     6301 tcgtttgtat aaaacaagtt ccagatacaa cagaagttaa actagatcct aatacaggta
     6361 ctttaattag agatggagta ccaagtataa taaaccctga tgataaagca ggtttagaag
     6421 aagctataaa attaaaagaa gaaatgggtg ctcatgtaac tgttataaca atgggacctc
     6481 ctcaagcaga tatggcttta aaagaagctt tagcaatggg tgcagataga ggtatattat
     6541 taacagatag agcatttgca ggtgctgata cttgggcaac ttcatcagca ttagcaggag
     6601 cattaaaaaa tatagatttt gatattataa tagctggaag acaggcgata gatggagata
     6661 ctgcacaagt tggacctcaa atagctgaac atttaaatct tccatcaata acatatgctg
     6721 aagaaataaa aactgaaggt gaatatgtat tagtaaaaag acaatttgaa gattgttgcc
     6781 atgacttaaa agttaaaatg ccatgcctta taacaactct taaagatatg aacacaccaa
     6841 gatacatgaa agttggaaga atatatgatg ctttcgaaaa tgatgtagta gaaacatgga
     6901 ctgtaaaaga tatagaagtt gacccttcta atttaggtct taaaggttct ccaactagtg
     6961 tatttaaatc atttacaaaa tcagttaaac cagctggtac aatatacaat gaagatgcga
     7021 aaacatcagc tggaattatc atagataaat taaaagagaa gtatatcata taatttaagg
     7081 ggggataaag aatgggtaac gttttagtag taatagaaca aagagaaaat gtaattcaaa
     7141 ctgtttcttt agaattacta ggaaaggcta cagaaatagc aaaagattat gatacaaaag
     7201 tttctgcatt acttttaggt agtaaggtag aaggtttaat agatacatta gcacactatg
     7261 gtgcagatga ggtaatagta gtagatgatg aagctttagc agtgtataca actgaaccat
     7321 atacaaaagc agcttatgaa gcaataaaag cagctgaccc tatagttgta ttatttggtg
     7381 caacttcaat aggtagagat ttagcgccta gagtttctgc tagaatacat acaggtctta
     7441 ctgctgactg tacaggtctt gcagtagctg aagatacaaa attattatta atgacaagac
     7501 ctgcctttgg tggaaatata atggcaacaa tagtttgtaa agatttcaga cctcaaatgt
     7561 ctacagttag accaggggtt atgaagaaaa atgaacctga tgaaactaaa gaagctgtaa
     7621 ttaaccgttt caaggtagaa tttaatgatg ctgataaatt agttcaagtt gtacaagtaa
     7681 taaaagaagc taaaaaacaa gttaaaatag aagatgctaa gatattagtt tctgctggac
     7741 gtggaatggg tggaaaagaa aacttagaca tactttatga attagctgaa attataggtg
     7801 gagaagtttc tggctctcgt gccactatag atgcaggttg gttagataaa gcaagacaag
     7861 ttggtcaaac tggtaaaact gtaagaccag acctttatat agcatgtggt atatctggag
     7921 caatacaaca tatagctggt atggaagatg ctgagtttat agttgctata aataaaaatc
     7981 cagaagctcc aatatttaaa tatgctgatg ttggtatagt tggagatgtt cataaagtgc
     8041 ttccagaact tatcagtcag ttaagtgttg caaaagaaaa aggtgaagtt ttagctaact
     8101 aatttcttgg ggagggtatt atgagtacaa gtgatgttaa agtttatgag aatgtagctg
     8161 ttgaagtaga tggaaatata tgtacagtga aaatgaatag acctaaagcc cttaatgcaa
     8221 taaattcaaa gactttagaa gaactttatg aagtatttgt agatattaat aatgatgaaa
     8281 ctattgatgt tgtaatattg acaggggaag gaaaggcatt tgtagctgga gcagatattg
     8341 catacatgaa agatttagat gctgtagctg ctaaagattt tagtatctta ggagcaaaag
     8401 cttttggaga aatagaaaat agtaaaaaag tagtgatagc tgctgtaaac ggatttgctt
     8461 taggtggagg atgtgaactt gcaatggcat gtgatataag aattgcatct gctaaagcta
     8521 aatttggtca gccagaagta actcttggaa taactccagg atatggagga actcaaaggc
     8581 ttacaagatt ggttggaatg gcaaaagcaa aagaattaat ctttacaggt caagttataa
     8641 aagctgatga agctgaaaaa atagggctag taaatagagt cgttgagcca gacattttaa
     8701 tagaagaagt tgagaaatta gctaagataa tagctaaaaa tgctcagctt gcagttagat
     8761 actctaaaga agcaatacaa cttggtgctc aaactgatat aaatactgga atagatatag
     8821 aatctaattt atttggtctt tgtttttcaa ctaaagacca aaaagaagga atgtcagctt
     8881 tcgttgaaaa gagagaagct aactttataa aagggtaaaa atctaattaa aaatatattt
     8941 gctgttaact agattaaata gtattttaat ctagttaaca gaatataaat actaaaataa
     9001 caggaggtta taactatgaa attagctgta ataggtagtg gaactatggg aagtggtatt
     9061 gtacaaactt ttgcaagttg tggacatgat gtatgtttaa agagtagaac tcaaggtgct
     9121 atagataaat gtttagcttt attagataaa aatttaacta agttagttac taagggaaaa
     9181 atggatgaag ctacaaaagc agaaatatta agtcatgtta gttcaactac taactatgaa
     9241 gatttaaaag atatggattt aataatagaa gcatctgtag aagacatgaa tataaagaaa
     9301 gatgttttca agttactaga tgaattatgt aaagaagata ctatcttggc aacaaatact
     9361 tcatcattat ctataacaga aatagcttct tctactaaga gaccagataa agttatagga
     9421 atgcatttct ttaatccagt tcctatgatg aaattagttg aagttataag tggtcagtta
     9481 acatcaaaag ttacttttga tacagtattt gaattatcta agagtatcaa taaagtacca
     9541 gtagatgtat ctgaatctcc tggatttgta gtaaatagaa tacttatacc tatgataaat
     9601 gaagctgttg gtatatatgc agatggtgtt gcaagtaaag aagaaataga tgaagctatg
     9661 aaattaggag caaaccatcc aatgggacca ctagcattag gtgatttaat tggattagat
     9721 gttgttttag ctataatgaa cgttttatat actgagtttg gagatactaa atatagacct
     9781 catccacttt tagctaaaat ggttagagct aatcaattag gaagaaaaac taagatagga
     9841 ttctatgatt ataataaata agaatttggg aattaaaagt ttaaataaaa ttgtttaaaa
     9901 aacaatttcg gatatatgaa aaatctaatt taatgggggt aatgaatatg agagaagtag
     9961 taattgccag tgcagctaga acagcagtag gaagttttgg aggagcattt aaatcagttt
    10021 cagcggtaga gttaggggta acagcagcta aagaagctat aaaaagagct aacataactc
    10081 cagatatgat agatgaatct cttttagggg gagtacttac agcaggtctt ggacaaaata
    10141 tagcaagaca aatagcatta ggagcaggaa taccagtaga aaaaccagct atgactataa
    10201 atatagtttg tggttctgga ttaagatctg tttcaatggc atctcaactt atagcattag
    10261 gtgatgctga tataatgtta gttggtggag ctgaaaacat gagtatgtct ccttatttag
    10321 taccaagtgc gagatatggt gcaagaatgg gtgatgctgc ttttgttgat tcaatgataa
    10381 aagatggatt atcagacata tttaataact atcacatggg tattactgct gaaaacatag
    10441 cagagcaatg gaatataact agagaagaac aagatgaatt agctcttgca agtcaaaata
    10501 aagctgaaaa agctcaagct gaaggaaaat ttgatgaaga aatagttcct gttgttataa
    10561 aaggaagaaa aggtgacact gtagtagata aagatgaata tattaagcct ggcactacaa
    10621 tggagaaact tgctaagtta agacctgcat ttaaaaaaga tggaacagtt actgctggta
    10681 atgcatcagg aataaatgat ggtgctgcta tgctagtagt aatggctaaa gaaaaagctg
    10741 aagaactagg aatagagcct cttgcaacta tagtttctta tggaacagct ggtgttgacc
    10801 ctaaaataat gggatatgga ccagttccag caactaaaaa agctttagaa gctgctaata
    10861 tgactattga agatatagat ttagttgaag ctaatgaggc atttgctgcc caatctgtag
    10921 ctgtaataag agacttaaat atagatatga acaaagttaa tgttaatggt ggagcaatag
    10981 ctataggaca tccaatagga tgctcaggag caagaatact tactacactt ttatatgaaa
    11041 tgaagagaag agatgctaaa actggtcttg ctacactttg tataggtgga ggaatgggaa
    11101 ctactttaat agttaagaga tagttttaga ttataatatt tacaaattaa atatttaatt
    11161 atgaatttaa cctgtaagaa taactatttg ttattcttac aggtttttat agttaaaaat
    11221 aattattatc tattaaacaa aaatagtgta aaatatataa tatcatgatt ttgaagatat
    11281 aaaaaagtag gaagatataa aagaaagtag gtaaaagttt gtttaaaaaa gagaatcaaa
    11341 gatataatct tatatcgtat acaaatgaag aagaaatgac tttaaaagaa gttttattgg
    11401 acaagctaaa tttctcagtt aggtcattat caaaaatgaa gagggaaaag agtgttttag
    11461 taaatggagt atataaaaaa ccgagtttaa aggtgtatag tggtgattta atagaagtaa
    11521 aaatagacga ggaaaaagca aattttgaac ctcaagattt aaatttacag ataatttatg
    11581 atgattttga tataattatg gtgaataaac ctccgtttat ggtagttcac ccaacaaaaa
    11641 gccactatga taaaacaata gcaaatggta ttagttacta tatagataat caaaaagaaa
    11701 atgtaaaaat aagatttgta aataggttag atatgaatac ttcagggtta gttatagtag
    11761 caaaaaatgc ttatgcacat catactttgt ctacagcaat gagtgaaaat aaagttgaaa
    11821 aaaagtatat aacagtagtt gatggaatta taaaagaaaa tgagggaact atagatgagc
    11881 caatttatag acctacagaa gattcaataa agaggataat agatgaaaga ggacagtcat
    11941 ctgttactca ctataaagta atagaaagac tagaaaatgc aactgtatta gaagtaagct
    12001 tagaaactgg tagaacacat caaataagag tacatatggc tcatataggt catggaataa
    12061 taggtgatga gttgtatggt tatgtagatg aagatctaat aaataggcaa gcacttcatg
    12121 catataagct agagtttgaa caaccaagaa caaaagaaaa attgaaattt aaagcagata
    12181 taccagagga tatgaaagag ttaatatcga aattaagata atggggtgat atatatgata
    12241 atacataaat ttataataca tgttttggac aaaaatagtg atgttccaat attaaatgac
    12301 tttgaaggca aagttaatca agaagtagat ggttttttcc agaaagctat aaaaagaatt
    12361 gcaaaagatg aggatttaag aaaaggtgtt tttaaagatt ataatgacaa tttaattaaa
    12421 aactgttgtg aacaaataat atatgatgaa agtacttttt tgaaaaattc taaagaaatt
    12481 gcatcttact tgtttgatgt tatgaaaatt aatgctgcac tagaatcttg tgatttggca
    12541 atttgcttat acactattaa agatgaaaag agtgtagcaa tactgaaatt agattataaa
    12601 aagttgtaca ctcattcaat tgaatttgtg gatgataaat ttaatattca aatggtttca
    12661 aatgaaattg gtatcccaga gactttaaga caaaagcaag gagctttgat tagtctaagt
    12721 ggtataaatg atgagtttca tctaaggctt ttagataagg atgctgaaaa agaagggtca
    12781 gaatctaagt ttgtaacaga atttttaaat gctaaaaaaa tagatgatga taagtacaag
    12841 accaaggtat ttaaaaatac agctgaaaat tggataacca atgctcttag taatgatata
    12901 aagcaagctg aagatgtaag aagtatatta aattatacac tgaaggaaaa acatgaagtt
    12961 gatataaatg actttgttga taattcaatt aaagatgatg agttaaagga tagctttaaa
    13021 gagcatatgg aagaaaaagg tcttgacgaa agttttagta tagataaaaa atgggttgag
    13081 aaaaagctta aaaaaagaag tataaaaacg gataatggtt ttgacataaa agggaatcta
    13141 actgattttg aagacccaat gaagtatact gtaaaacaaa atcaagatgg aactatagat
    13201 ataataataa agaatgttac gttttatgaa gaaaagtaat ttatatactt agtatataaa
    13261 ttaaatataa gagcgtgtat tttatatatt gtaaataaat gtaatctagt ctgtgcgaac
    13321 tctaatataa tttattatgt agaagtttac acagactatt tatatttgta gacaaaacga
    13381 tactatttgt tttcttcgat atatttacat atatcaccaa tacatttaaa gttttcagct
    13441 tcctcgtctg gtatttctat tccaaattca tcttcaaggg ccattataat ttcaacagca
    13501 tctaatgaat cagcttctaa gtcgtccatt aaagatgctt ccatagttat ctcatctaaa
    13561 ttgtctaccc ctaattgttc tgctattatt tcttttattt tctcaaacat tttaagcctc
    13621 ctaaagcagt tttaattaat tttaatgtta gtttaatata atatcaagaa tttttcaata
    13681 tggaatctat aaaaaataat aaaattttat aaatgtagta ataaagtatt atttgatgaa
    13741 taaactttat tgataacaaa tataacttag gaggaaatgc tatgaaatat gtaggactat
    13801 tactaacttc agtaggaatg tttttactta tagcagtaaa tttttattat aattcaataa
    13861 ctttagatat gcaacgtata gaggattatg taatggagac caatttaatc cttgaagatg
    13921 tagctgaaaa ggagagttat gtatctaatg aaaaagaaga ttacatatca aggttaatgc
    13981 atgtaaagaa aggaatagaa aattcaaaaa catccttctt gatagaaaga tataaggagt
    14041 acaaaataaa atcaatagaa agtttaatct atactatatc agaggagaaa aaagattatc
    14101 tggatgaagt tgatagatac aataaacttg gtgaaaaaga aataaataaa ctaatcaata
    14161 aaaacttttt agaagtaact tatctgtcta ttactacata tatttaatta aagagacaaa
    14221 acttagtctc ttaatgattc aaggaggatt agttatgaca aaattagaag acaatatgga
    14281 acgtttcttt ggaggatgcg aaggattttt tgggaataaa atgttgataa taatagttat
    14341 agtattctta ctattatgca cagacatttt agaagactta ttatgcgatg acaatatatg
    14401 gatatgggtt atattaatag tattgttatt atttaacttc gatgatggtt gttgttgcta
    14461 aaaaatattg attattaaat aggtaaaacc agtaacttat acaagttatg gttttacctt
    14521 ttttagttca aattaaaaaa tttaaaaaaa tagtaaaaat acattagcaa aatggaaacc
    14581 aaatgcatag tatatgttag gagcgaaaag ctcaacaaaa tatcaacagg aggtaacgta
    14641 taatgttaga tagattcttt ggagaagaag gatgttttgg tggaggagga tggtggataa
    14701 tagtactatt cttcctattc ttagcttttg gagatagctg ggcagaattt gacataatgg
    14761 catggatacc attcctaata gttttattaa tactttgctg ctgtggtgga tgcttagaat
    14821 aagaacacag ttattaaaaa aaggagggct ttgttatgtt agatagattt tttggagaag
    14881 aaggatgctt tggtggagga ggatggtgga taatagtact attcttccta ttcttagctt
    14941 ttgaagattg ctggagagac attgacataa tgtcatggat acctttctta atattattgc
    15001 taataacttg ttcatgtggt ggattctttg atggtgaagt aggttgcggc tgctaatact
    15061 taaatcatta gaataaaaaa atgctggtat tttataccag cattttttta ttttattttt
    15121 taatatgatt taaattaaga atgaatatta ttaaatttgt tgaatatagg gtataatata
    15181 aaatataatg tatttaaatg tacttgcttt tatggtaaaa taaaaactta tataaatgta
    15241 gttaaaatag gaaccttcaa aaatttggtt cagttttaga taaatttagt aaaataagga
    15301 ggatgaagat gaaaggcaat ataacgataa aagatgttgc taaacaagca ggagtgtcaa
    15361 tatctactgt atctagagtt ataaatgatt caaaacctgt aactgatgaa gtcaaacaaa
    15421 aagttttaga ggttataaaa gagactggat atataccaaa tccacttgct agaagcttag
    15481 taacaaagaa gagtcaatta ataggggtaa tagttccaga agtttcagat tcttttgtta
    15541 atgaggtgtt aaatgggata gaagaggttg ctaaaatgta tgactatgat attcttttag
    15601 cgaatacata ctctgataag gaacaagaac ttaagagtat aaatctattg agagcaaaac
    15661 aagtggaagg tatagttatg atttcatgga tagttgaaca agaacatatc aactatatac
    15721 aaaattgtgg aataccagcg acatatataa gtaaaactgc tagaaattat gatatatata
    15781 cagtaagtac tagcaacgaa gaagctactt ttgatatgac agagcatctt ataaagaaag
    15841 gtcatgaaaa gatagctttt ataatgacta gtaaagatga tactgtttta gaaatggaaa
    15901 gacttgctgg ttatgagaaa gcactttcaa ataacaatat agaattagac aagagtttga
    15961 ttaagtatgg tggaactgat tatgagagtg gatacaatag tatgaaagaa ctattagatg
    16021 atggaataat acctcatgcg gcttttgtaa caggtgatga ggctgccata ggtgctataa
    16081 atgctatatg tgatgctgga tataaggttc cagaagacat atcagttgca ggatttaatg
    16141 atgttaagat agctagaatg tatagaccta aacttactac agtatatcaa cctctatacg
    16201 atatgggagc agtagcaata agaatggtta taaaattaat aaataaggaa ttaattgaaa
    16261 ataagaaaat agagttacct tatagaattg ttgatagaga aagtgttaca gaaagaaaaa
    16321 aataaaacta ttaaaatcaa tcaggtagtg ccttaggcac tacctgattt tttatgtcaa
    16381 taatatcaaa tttaataata gattaactaa aaataattat ctaattgctt aaatagttta
    16441 tttttatcta ttcatgagga ttagactttt tagttttctt aaataaactt ttagattttt
    16501 tttgttgact acctggtgta ttttttggtg caggtgaatt tgacttacct ttagtaggtt
    16561 tagccttctt agttttagct ggtttagtat ctctttggtt ttctgcatct gagatttttt
    16621 ccattatatt atccagtttt ttgcgttgat tttcatctaa aaatgctttg aattttgata
    16681 ctacttcatc tctgccatct agattcttac ctatttttat aagttcctcc ataagttcat
    16741 tttctgattt tcctttgtaa ctatcagcca tcttttctat tttatccttg tcaatatttt
    16801 tttgtttagc cattttttct aatgactctg tatctaattt tttcataaaa aaaccccttt
    16861 ctataaatat atattgaatt atattcatac tatttatata tagaaataat attgaagtgg
    16921 tgaattgtga gtgatatata atataaataa aaattcttat agataatata tatgcactga
    16981 gctttaaaaa tggaacatta aaatatgaaa aacatacttt aatatagggc aattatataa
    17041 tataaggggg aattgtcttg aataacttac tatttttgat tggagtgatt gcattgagta
    17101 atggtaatat ctctatattt gatgataaaa ataaatcaaa aggaaataat agagttagaa
    17161 aaaacaaaaa aaatgcaaat gataaacttt cacagtctac acatggcaat ttaaggtttg
    17221 atgcaaatga tataaaaaga ggtttaaggc tcatggattt aagtgaagaa gatttagaaa
    17281 tgggaatgga aataataaca agaactaaaa aatacatgtc aagagatgaa agaaaaatac
    17341 taataaaaat agaaagtgtt ttagatttag ttagaggaat aaaaaaatta aataatatag
    17401 atatggtaga tacagaagat gaaacggatt ttttcagaaa aatggatagt gatgataaaa
    17461 agaatatgat gataaaggaa attatagatg tattcccaga aaaaagaaaa aattcagttg
    17521 aaaaagctat aggaatgaaa aagaagatag atttatttgc agaattattt ttaccagatg
    17581 actttggtga aggtggattt agtttgagct ctttggcaaa tataaacaat cttggtagta
    17641 tgaataatct caaattgtta ggaagtcttt taagaggtga tgatagtaat aatgatgatg
    17701 aagacaataa tgatgagtta aattatgaag acgaagaatt agattgtgaa gacaaagaat
    17761 tggatgatga tgagttaaca gatgaaggtg aaatggagga taatgaagat gaattagtag
    17821 aagataagaa cacaggctat gaaaatatag aaaatgatga agatatagta tacgatgagg
    17881 aactatacaa cattaatgag caagaatccg aatacaatta taatgaaaaa aatactaaaa
    17941 ctagagctaa gaaaaaacga taattaacta aatacataaa gaaaaaagga agaaaaagta
    18001 atgatagaat aagattatct caattactat ataaaaccac tttaaaaaat tcaaaattta
    18061 ttatataata ataatatgaa atcctgaaac atacgataag gcttttttaa attcaaccta
    18121 caaatgaaat acgggagacc gtgtctggta aattataaca tgcctagtta gtttggacgt
    18181 taaaggttac tggcaagtca cccacctggg agtgcctagg gtgtgaattt acttagccac
    18241 cggtgtttcg ggacaaaaaa agtgctacta tttcaaatag tagtactttt tttattaata
    18301 tgcatttttc tactaaatac gcctaattat tgataaattc aaaaagcaag taacaattaa
    18361 gattataaaa gcatagtatt aattaggctt caaaaagcca aagacttaat aagaatcgct
    18421 tacagggagg gcaatgaaaa atgcaagatt ataaaaaaaa taaaagaaga atgatgaatc
    18481 agccaatgtc tacaatgaat gaagaagaag tgtatacaga tgaaataaat tcagaagaca
    18541 tgagaggttt taaaaaatca caccatcata atggatgtaa tactgataat aagtgtgagt
    18601 gccatgatga ttgcaatcca tgcaacccat gtaatccatg taaacctaac ccatgcaatc
    18661 catgcaaacc taatccatgt gatgacaatt gtggatgcca tgacaattgt aaatgtgatt
    18721 gtgaaccatg tgaaatggat tcagatgaat gttttgaaaa caaatgtgga ccagaatgct
    18781 gtaatcctat atctccaaga aacttctctg tatcaaatgc agtgccattt gcaatagagg
    18841 ctaatagaat atttgatact atgcaattcc aaacatttac agatgcaaca ggaccaaatg
    18901 gagagccatt aacttttgaa acagaagtag tagaagtatt tggttcagtt ccaagtgcag
    18961 gtcaagcaag tgtaactata gaaaaaatat gcttaagtaa tgatggaatc gttatagaca
    19021 caggaatgac aactttagaa gatttcgatt tagacccatt aggagatata gtaggaagaa
    19081 actgtgaaac aacttttgaa tttgcagttt gtggagaaag aaactctgag tgctgtagac
    19141 aaggaaaagg caaatcagta gcttataaac aaagaggatt aactgtagca gttcgtaatt
    19201 tagtactaga gctaagaggt agatgtggat gtacagagtt cgttgcatta gctttcccag
    19261 cagttagagc aggaggtgga tgtaagagaa gagttgatta tgtagaattt acttttaaca
    19321 cactttcagc accaatatgc ttgccagctg acggaagagc tgttacttta agacaagaat
    19381 atcaaactaa cttaactgta gattgtatag gaaaatctat attaaaatta gaatgcaacg
    19441 aatgttgtga acctttctat gaattaatta taccaaatga tatagattta gtactttgct
    19501 tacaagaaac agttagcaca ttaataagtg aacaaatagt agttttagca tcaccaaatc
    19561 caatccaacc aagacttgtt gatactttct ctaaagtatg tgatttttcg caatgtggac
    19621 ctaatcatgg aagtggaaag ccaagttgcc acagatagat aggtagatat atagacagtt
    19681 taaattataa ataaaaatca tctaaggaag gctaccaaag tgtataattt acattggtag
    19741 ccttcacgtt tttataacta gcgtatagat tttagtgatt taaaaagcac tatagtatgt
    19801 aatagacaag taaagattga tactatcgtc attgtgtata tgtatgcata aatattaagt
    19861 cctggaattg gaagaaatac atataaagct attagttgaa ttaacatgcc agtaatagta
    19921 tttatacttg aagctaattc ttttcttatt gaatgtaaaa tgccagatag agtttgattt
    19981 agggacataa atagtggaac taaaaacata gcttttagat attctcctgc aagtgtattt
    20041 ttaaatacta atatacataa atcttttcca aagaaataaa aaaatattga tgatagtatg
    20101 cctacaaata aagtaagaag tatggagtat ttgatttttt taattacact cttacgcttt
    20161 cttagagcca tttcttgtga aatagttggt attaaattta caataagcgc tgaaccaaca
    20221 gtaaaaggta aaaatataaa aggcattacc atcccactga caataccata catacttaaa
    20281 gattgttgat aagtcattcc tgataatgct aatcttgatg gtatcatcat agaacttata
    20341 gattgcaaaa gtatgtttga cattctatta caagttatgg gaatagacat ttttaatgtt
    20401 tccatagaag aattataaaa atcttttatg cttataatat acctattgtc aaatgatgaa
    20461 tctcgccata agcaaataag cataaatata atattactca actcaccaat cgatatacct
    20521 aatagagcta tataacaatt catagattta ttatttatgt acataacaag taaaaaaaca
    20581 aataatattt taccaatttg ttcaatgatt tgtccaatag cagggatttt tacattcttt
    20641 atgccatagt aatatcctct tagtacgttt gaaagagtta ttataactat agcaggacaa
    20701 acagctagta taaatagatt taatttagca tcttttaaaa atttaagtga taaaaaagaa
    20761 ctattgaaag atgcaactat agatataatt agtgctacga aaaatgatat atataaagtt
    20821 gatataaata aagcattagt attgtgtcta tcgtttaaag cttttctctt tgcaactaga
    20881 caactaagtg cagtaggtat acctgttgtt gtaacagcta agcaaatcat caaaaagtta
    20941 aaaatcatat gatagatacc taaacctgtt tcacctagtg ctcttgataa gaatatttta
    21001 tataaaaaac caaatattct tacaataaaa ttggatataa ataaaataac tgttgatagt
    21061 actaaataag gtactttcaa aaaaatcacc tccttatctt ttaaatatat tcacaaggag
    21121 gtgtaaaaat ttataatttt ataaattctg gtttcaattt attaaattta gatacatatt
    21181 taaatcccat attttttagt aatgaatata tgtagtcaaa ctcacatgca acttgactta
    21241 tatgatgtga atctgaacct gtggttatga tttcccctcc caagtctttg tacatttgaa
    21301 gtatatactt agatggggtt aggtctggaa gattatatct ataacaagag gtatttatct
    21361 cgataccttt gccatcataa atagcttgtt taagagtttc ttcaattata tcagaaaata
    21421 gtctatcatc taaaatagtg tcataaggtg catacctttt aatcaaatct aaatgaccta
    21481 agacagagta atctttataa ttcttaacaa tattatataa ataaagatag taattttcat
    21541 aaacttcatg ttgagtttta tctttaaaat aattaccaag gtataagtcc attgtatcta
    21601 tagcatggat agaacatatt ataaagtcca atggatattg atgtgcttcc tttttgtatg
    21661 tatccattaa ttgagtttga actccaaatt caattccttt ttttattgat attttatctt
    21721 tatatttgct ttgaagagtc tctaaactct taaaataatc ttcgtagaca attgaaaaag
    21781 aatcatcagc atatacatca tagtcaacat ggtctgtgaa acaaatttcc tccaaaccga
    21841 gttcaataga tttttttacc atttcttcca ttgtagattt tccgtctgta gagaaacttg
    21901 agtgcatgtg atagtcgtaa aacataaata tttccccttt ttataaaaat tttggtatta
    21961 tatatacata ctaaataata tcaaatatac ataaaagcat caataaaata taaatacaat
    22021 gagcggtgat aaatttggaa aaatacagaa tagatttaaa tgagaaaaaa tcaactaaat
    22081 atatacagat atttaatcat ataaaagaat tgataataaa taaagagtta gtagaacatg
    22141 aaaaattgcc tccaataaga aaactatcaa attttttaca agtaaataac actactatag
    22201 ttaaggtata cgagctttta gaaaatgaag gatatgtata taaaattata gggagtggta
    22261 cttttgtatc aagtttaaat tcttataaaa gcggtataga taacaggaaa aataagatta
    22321 ttgtgcaaag agaagattta attcattttg acaatgggaa tccatctaat gatatgtttc
    22381 ctatcgattc atttaaaaat gctgtaaata tggcattatc taaagatggt agttcaatat
    22441 ttgaatatga tgaaggtctt ggctctgaag aattaagaga aaaaatggta gagtatctaa
    22501 gtgatggaaa tataaaaact actaaggaaa atattcaaat aatatcaggt gctcaacaag
    22561 gtatagatat agtttgtaaa ggacttataa gttattcaga tgttgttttt atggaagaac
    22621 cttcttacaa tggagcaatt gaagtgttta aaagtagagg tgcaaaaata atatctatac
    22681 ctatgttaga tgatggaatt gatataggta ttttaaaatt aaagttggaa aaaataaaac
    22741 caagacttat atacattatg cctaattttc aaaatccaac tggaatatct tactcaacat
    22801 ataaaaaaaa gaaattgata gaattggcag aagaacatga tttttatata atagaagatg
    22861 attttataag tgattttgtt tttgaatcaa aggacaatag aacaattaga agttatgatg
    22921 ataaaaatag agtagtatat ataaaaagct tttcaaaaat acttatgcca ggtcttagga
    22981 taggaattgt agaaatgcca aatgagttgt taaaaaaagt tttatgggct aaatattcat
    23041 cagatatatc aacaccaggt ttaattcaaa aatcaatgta ttactatatg aataattttg
    23101 attggaagaa ttatttgagt catatagaga agatatatac tgataagtat aaattggcaa
    23161 aaaacttgat taatgacaaa ttgagtggta aattaaaagt tagaaaatct tgtggaggaa
    23221 taaacttttt tttagaatta ccaagaggat atacatctca agcatttaca aattttatgt
    23281 taaacaaagg agtgtcaatg cttccaggga cttatttttt tgacaatcta gtggatgata
    23341 gattctttag gataaatata gctcgtccaa gcatggaaga actagaaaag ggaatatcta
    23401 taataagtga taatatagat gagttttttt gtgaatataa aaacaagtta gatataaaaa
    23461 gcaataaatt attttattaa ataaatagag gttttttagg aggaaaaaat gtttgacaag
    23521 aagaaattag ataggattaa tgaattagca aagaaaaata aagagggaat cctttctgct
    23581 gatgaaatca aagaaagaga aattttaagg aaagaatatt tagaaaactt tagagcccac
    23641 tttagatcaa gactagatag tgtcaaagta gtttcaccag aagaatatga gcaatacatg
    23701 aaaaacaata aaaattagaa cttagtagga ggaaaatatg aacttaaagt acgaatttaa
    23761 gaatgcttgg gattttgaaa gaaaagaaaa tactattgaa caaattatgc aatattctag
    23821 caattatatg gagtttttaa gtaagtcaaa gactgaaaga ctttcagtaa aagaaattat
    23881 aaaattggct aaagaaaata actatatttc aatagatgaa gctatggaaa aaggaagcat
    23941 aagttgtggt gataaaattt atgttataaa taaagagaag gcagtagcac tttttgtaat
    24001 aggtaaaaat tatatagaaa aaggtatgaa gattatagga agccatatag attcgccaag
    24061 attagattta aaaccaaatc cactgtatca agagagtaat ctaggttttt ttaagactca
    24121 ttattatggt ggaataaaaa aataccaatg gacggctatt ccacttgctt tacatggtat
    24181 agttatatta aatgatggaa caaaagtgga catatctatt ggggaagaag atagtgaccc
    24241 agtattttgt gtaactgatt tattgattca tttggctgga gaccaaatgc aaaagaaact
    24301 atcagaagga attagtggag aagctttaaa tatattaatt gggaatatgc ctttagaaga
    24361 tgaagaaaaa gaacctataa cagcaaatat attaaagatt ttaaatgaga aatacaatat
    24421 agttgaagaa gatttattaa gtgctgaaat tgaagtagtt ccagctggta aggctagaga
    24481 tttagggctt gataggtcta tggtgttggg atatgggcat gatgataggg tatgttcata
    24541 tgctgctgta aaagctatac tagaaactga acaaccagaa tttacttcag ttgcattatg
    24601 tgtagataaa gaagaaattg gttctaaagg gaatacaggt atgcactcaa agttttttga
    24661 aaatactgtg gctgaactga ttgctttaga aggagattat tgtgatatta aagtaagaag
    24721 agctttagca aatagtaagg tattatcagc tgatgtttct gctggatatg acccaaattt
    24781 tggagaagca tatgaaaaga gaaattcggc atatatggga aatggagtag ttttaactaa
    24841 gtatacaggt tcaagaggaa agagtggatg taatgatgct aatgctgaat ttatgtcaga
    24901 agtaaagaga atttttaata aaggaaatgt agtatggcaa actgctgaac ttggaaaagt
    24961 agaccaagga ggaggcggaa ctatagccca tatattggca aatcaaggtg cagaggttat
    25021 agactgtggt gttggagttt taaatatgca tgcaccacat gagattgtct ctaaagtaga
    25081 catatatgaa atgtataagg gatataaggc attttttaat ataaatttat aagtaaaaat
    25141 ataaatgtaa taagtgaata aaggctgttg tataaacaat ttaatctgtt gtacaacagt
    25201 cttattttta tgtaaaaata gcaatttgtc acactagaga aaataaaaga aaatactgtc
    25261 aaattaaaaa ttattttaaa aaaaattgac aacgatttca tcttgttata atatgtaata
    25321 aagaacttgt tatataatgt tataaaaatg ttacataggg aggtgagcaa aatggatgaa
    25381 aagagacaaa aaaaagaaat ggatttagaa gtaaattcct ttgttccttt atatcaacaa
    25441 ctatatgaca acataaaaaa acaaatagca tctggcatat ataaaccagg agataaactt
    25501 ccatctgaag gagacttatg taaagaattt aatataagtc gtataactgt gagaaatacg
    25561 acttttttat ataaaatagc aaaataacag tgtttgagca aaaaaaaaga atgtttttta
    25621 tcgttttgcc acctgtttgc caccataact tttttcggtg gcaaattatt gtaaaatgcc
    25681 ctccaaaata tctattgttt cactttccat tttacttgta acatgtgaat atgtgtccat
    25741 agtggttgat aattgactat gacctaatct ttgttgtata tattttacat tagctccatt
    25801 ttctaataat aatgtggcat gtgtatgtcg caagcaatga aaattaaagt taatatttag
    25861 tttcttgtta atagttctta tagcagcgtc aatattgttg tgatttacaa atgagccatc
    25921 tttttttcta caaacccaat catactcagt ttctttatac cattttccaa ttttaatttt
    25981 ttgttttttt tgattcaatt tttcctcttt taatatccta gacaaagtat cacctatttt
    26041 aatatctctt attgatgttt ttgtcttagg tgatgctaat tcaaattctg aaacttttct
    26101 ctttattaaa ttttttctaa ctttgattat attattatct aaatcaacat tatcccaaca
    26161 aagacctaat atttctcctt ttctcatgcc tgtatgaaac cctataagta gaggaatata
    26221 gatatttgta tttttaggat atatttctag tattttattg aactcatcta gtgttatagt
    26281 cttgttatct gactcatttt ttacctttaa aacatttttt ggtatactgg catattgaac
    26341 aggattttcc ttaatgagtt tgtaagggta aacagcagat tttaaagcag catttaatac
    26401 aacataaatc gcttttaaaa ctccttttgt gtagtgtttt tcttctccgt tttgtgtgta
    26461 tgtctctttt gatttattat ttaaaaattc ttgaataata gctggattta tagattttag
    26521 cttacattta ccaagtctag gttctatatg attttcaatt aaatttctat aactttcctg
    26581 agtattgtat ttgcaattaa gaagtacata ctctttgtac caaaagttaa gatagtctga
    26641 taaactcata tttgtttcat caaacactat accagcattt tcatattcat ttattgcatc
    26701 acggagcgat ttttcagctt ctttcttagt gtttccacca actctttcta ccttttttct
    26761 cttgccatct actatacctg catcaaagta gtaataccac ttctttccac gttttcttac
    26821 gccgcctttc ataaaatctc tcctctcaaa agcttttttt atgtaattat ataataacat
    26881 atatcaattt aaataaaata aataaaaaag actataagaa acaaagtttt atgtttataa
    26941 ttaatagtct tttttattta gatacatatt taatgttttt tatcacgtga ttccagcact
    27001 ttttctgcca aataaacaac tattttttca tactcaatat cagtttctaa agaaaagtca
    27061 acaatagcag gtatcgtcat atctcctgca tatttattac ctaaccaatt ttcaattaat
    27121 tccaattctt ctttgcttaa ttcaaatttt ttctttttat taaaacttaa gttcaattta
    27181 ttcacctcaa ataatattat atattaaaaa tagtatttga tagaaacatt cgaattattt
    27241 atgtaatttt ctttttttaa tatattcatc agcaaaataa tttactaaat atttttcctc
    27301 caatcctaaa gctatttcta attcaataag agcagggata gttaaatctt tattctcatt
    27361 attttccaac ctagaaattt gacttctgtg gcaaccaact ctttttgcta actctatttg
    27421 tgttaatttc ttcttttttc gtaactcttt taacatatat ttaacctacc ttttagataa
    27481 ttttatatat gttagtttgt gtaatttctt gtaataaatg tgcaatattc gcacactttt
    27541 tgttggaaat ttgtgctaga atgtagttaa gaaataactt tatctagata aagcaaaaat
    27601 aatagaagct ataaaatata ataatatttt acagttattt tatagtttta agctttgaaa
    27661 aagaggtggt tgtaactatt tttaagaacg tatgtttcgt ggaattaata aataaaatat
    27721 tttatagggg atgatatatt tgaataagac gaaatattat gatattttaa agttaaactt
    27781 attaatgaaa aagttgaaag aattggataa aaataaaatc aatgaatata aaataaaagt
    27841 gaaagaaata cataaaatca ataaaaaaga ggaatgattc ctctttttta ttatattcat
    27901 tttcagaaat aaactatttt ttattttttt cgtctagtaa aaataattct gcaactttta
    27961 atgctttttc tcttgcgtct gggcttagct cgctaaatat attaaaaact tctttcatat
    28021 cttcgtcaag atacatattc tcaataagct ccttttctgt tttaaaattt tcagcatcat
    28081 aatctttttc gtttttattt ataaataagc tatttctaac atcagttctt cctaataaat
    28141 aatctgtaga cacatcaaaa tagtttgcat attcttctat tgtacttttc ttaggttctc
    28201 ttaaaccatt ttctatcctt gatagtgtag atttgtttat atgtaaatcc tcgcttaatt
    28261 tgtctagcga gatgcctttt tcttctctca attcttttat cctattcaat ttgcacaacc
    28321 cctttttata ttaattccaa aatagcaact tttatttata ttatagcaac aaaattaatt
    28381 attacaattt tttttgctaa aaaagcaaca aaagtattga ctataatcac aaaacttgat
    28441 attatataaa tatgaagttg ccaaaatagc aaaaaggaag tgatttaatg tacttaaata
    28501 gattagaagg attgatgaag gaaaatagac acacccaaaa aaatgtggca gatatattag
    28561 gtcttagctc atatggtttt agattaaaat taaaaggaaa aaacgaattt aaagcaagtg
    28621 aaataaaaaa gatatctaaa ttatataatg tatctgcgga ttattttttt tcagatgaag
    28681 ttgctaaaat agcaataaaa gaagaaaggg gcaataataa tgaagaatct aaccataatc
    28741 aagcaaaata atcaattttt agttgaaagt agagaagtag cagaattaat agaaaagaag
    28801 cacgataatt tattaagaga tataagagga tacaagaaga ttttagagga ctcatcaaat
    28861 ttgaggagtc aagatttctt tatagaaagt acttatataa atactcaaaa taaaatccaa
    28921 ccttgctact tattaactaa aaaaggttgc gatatggtag ctaataaaat gacaggagaa
    28981 aaagggatta tatttacagc aatttatgta actaagtttg aagaaatgga gcaagagtta
    29041 aaagaacaac aacctaaatt accaactaca tataaagaag cactgcaaca gttattaatc
    29101 gaagttgaag aaaaagaaca actacaatta gaaaatcaag aaaaggataa ggtaatccag
    29161 ttacaacaac caaaagtact gtttgctgat tcggtagcgt cttctgacaa ttcaatccta
    29221 gttggagaat tagcaaaatt gcttagacaa aatggaattg atacaggaca aaatagatta
    29281 tttgactggt taagaaataa tggttactta ataaaacgta aaggtgagga ttacaataca
    29341 ccaactcaaa aaagtgtaga tttaggagtt atagaaacaa aagaaggtac aagagtacat
    29401 ccagatggtc atacaagtat tactaaaaca cctaagatta ctggtaaggg acaaatatac
    29461 ttcattaata aatttaaaag tagcaaacaa ttatcaatgt taagttaaaa cataacagta
    29521 ccttgaaaac taaatacaga atattcaaaa gaggtgattg aattgaaaca cgagcaaaca
    29581 acgcttcgta taccagaaga cttgtacaaa gcgttgatag atttaagtaa ggaaatcgga
    29641 atgcctattg catctattgt aataattgcg tgttggttgt atatatcaaa gataaattaa
    29701 ccaatgagat atgcaaaatg aatgcacagc attatttatt agagaattga ctgataaatt
    29761 attttcttta gctgttttct ctagcaactc ttttaggaca ccaggaatcc taagagtaga
    29821 acgaacagta tcatcacttt tatacgagat tgattgaagt tcattaactt ttgaatttct
    29881 aataatatca ttaatagcgt atagaataag cgaagatttg agaatgcctg tttgataaga
    29941 aattttttct aaattttcat ttaatggtgt atatattctt acagtaatag tagtcatttt
    30001 gacacctccc taatgacatt atacaaaaaa tatttaaaaa agtcttgaca ctaaaatagt
    30061 gtcataatat aatgattata gacagtgaat aagtgtcata aggagggagg aataagaatg
    30121 gaaaaagtaa gaatgacagt aaggctcgtc ccaaaattaa atcaatatgt acagaatata
    30181 gccaaagaat gtggaaaaag taaaaattct attattgtgg atgcatgttg ggagtttatt
    30241 gagaaaataa aaaaagaaaa taaattcaat gtagaaagcg aggaataaat atgaataatc
    30301 tacagatatt tgaaaaattg gaatttggtc aaataagaat ggttgaggtt gataagaaac
    30361 catattttgt tgcaacagat atagctaaat gtttaggata tgcaaacaca agtaaagcaa
    30421 taaatgacca ttgtaggtgg gtaacaaaaa gttacatacc tcatccgcaa aatgaaaata
    30481 agatattaga agtaaataca atacctgaaa gtgatatgta tagattgatt gtaaatagta
    30541 aactaccaaa tgcagaaaaa tttgaaagtt gggtatttga tgaagtttta ccaacaatac
    30601 gtaaaactgg aggatatata catacaacag aagatatgac agacgacgaa ataatggcaa
    30661 gagcattaca agtagctcaa aagacaatag aaaagaaaag cagagagata gaagaaaaag
    30721 ataaggtaat tcagttacag caaccaaaag tattgtttgc tgatgcagta gcatcctcgg
    30781 atgattctat attagttgga gaacttgcaa aactacttaa gcaaaatggc attgatacag
    30841 gcgagaaaag attatttgca tggcttagag ataatggtta cttaataaaa cgtaagggtg
    30901 aagattacaa tacaccaact caaaaaagtg tagatttagg agttatagaa ataaaagaag
    30961 gtacaagagt acatccagat ggtcatacaa gtattactaa aacacctaag attactggta
    31021 agggacaaat atactttatt aataagttta aaaagaacaa tcaattatca atgttaagtt
    31081 aaagattaat agtaccttga aaactaaata cagaatattt tgaaaaggaa gtgactacat
    31141 gagtaataaa aagaaatata ctttgagtat tacagaagaa ttatataata agtgcaaaga
    31201 aaaagctaat caaaaaggta tgtcagtaaa tgagtacata ctatcagtga ttagcaagaa
    31261 tttaaaaaat atttaatttt tattaactta aacattaatt ttgccattta ttttttcata
    31321 actttcaaca tgttttttaa ttaattgttc tatttctttg ttggcagaac gaccttcact
    31381 ttcagctata tacttgattt tttcaagtag attgttatct attctaagtg tgtatctcgg
    31441 taattttgat ggcataaata tagcaccttc cttaagtcta aatgatgtca ctattatacc
    31501 acgtaaattc tttcaaaaaa agtattgacg caaaagtgac gcaatgataa tataataaac
    31561 aaggaggtgg ttcaaaagtg acggcaaaga agagggtaac tgttagaatg ccagacgaat
    31621 taaatgaaga gctacataga caatcccaaa gaaaaggatt aagtaaaaat gcttttataa
    31681 taaatatctt atggaaagaa tttgaggatt tacaggactt aaaagatgaa caggaggttg
    31741 ataagtatga ataacttaca aatatttgaa aagatggagt ttggacaaat aagaatggca
    31801 gagattgata ataagcctta ctttgtagga aaagatattg caaagtcatt aggttataaa
    31861 aataccaatg atgcaatttt aagacattgc aaaggggtag tgaaacacga ggggtttaaa
    31921 attaatggta ttaagattgc tttgataact gagggtgatg tttacagatt aatagtaggt
    31981 tcaaatcttc caaacgcaga aaagtttgag agttgggtat ttgatgaagt tcttccaact
    32041 attcgacaaa ctggtcaata tcaagcacaa caaaatgtaa taacagaact tacaggaact
    32101 ataggagatt taaaaggaac aataaaagaa tataaaaagt tgtgtaagat aacatgctct
    32161 aagaaacaac agtattctaa atacataaag aatagactgg atatagacaa agctaataaa
    32221 gaatacaacc aagtgaaagc aaggctattc ctaattcttg gagttgagaa gtgggaagac
    32281 atagacttcg atacatctaa taatttaata caaattatag atgattgtat aaaggttata
    32341 aagtcagaaa gaccatacaa gcagttgagt tattttgaaa actaaataca gaatattcaa
    32401 aagaggtggt taaatgaaca ttaaaaaatt agtactagca acaggtgaag aatttgagaa
    32461 catagagctc aaagagtttg aaaataaagg agaatgctta ccatcaaata tggtaaaggt
    32521 tattgatggt aagcaggaac ttctaataaa taaagagttc atattatcat tagaagtcaa
    32581 aagaaagact actttttagg tcctttctta gtttgtgaca gagcactacc agcaaccgat
    32641 ttggaagcag cactatatct tccgtcttta agaatcttac tagccttaga agcaacagtt
    32701 ttacttgttt gtttagtatt tttagctata ggtatcacca cctttaaatg tatttatagg
    32761 atatatccta caaatatagt atatcaaagg aggaaaataa tggcaattaa tgacaacata
    32821 aataaaattt taaaagatag agatttaaaa gcatggaaat tagcaaaaga aataggtgta
    32881 gattcaggga atttatatgc aattttaaga ggagaaaata aaaatccaac tatagataca
    32941 ttaataaaaa tagccgacta tttagacatt acattagatg aactagttgg aagataaaaa
    33001 ttaaatacag aatattttga aaaggagtga gtaaattggg caatatatct aatttcaatt
    33061 tagataaaca agaagataaa agttttaatg acctagataa tatatcaatt tgtttttcag
    33121 aaggtatccg taaagttgta gaaatgaatt taaacaacta taaaaataaa atctcaaagt
    33181 acttaaatga aacttcgaaa atagaattat tagaaccaaa agaactaaca attgttataa
    33241 gtaaaggtta tcccgattat cttatgtctg ttgaagaagc aagtaaaaga ttgaaaatag
    33301 ataaagtatt tggatatgag ttaataaaaa atggactttt gaagtcagtt gatatagggg
    33361 caactaaagt ttctagttat gaattagatg attttataac taggaatcaa ggaaaaaaca
    33421 tcaaagaaat gcttagagaa atgaaagaac ttagagaggg ggtgatttaa ttggaactag
    33481 taacatacag aaacaagctt gttttactaa aagatggaga aaagattgca actataagtt
    33541 taaaaaggaa atttctcagc aacagactta aattaaaaat aagataggag agataaattg
    33601 aaaataattt ataaaaacaa agtttacaaa gtagaacaag acaaaaagtt atttagaatt
    33661 acatactatg atgagcagag aggtagtaag aagtttaata aagagaagaa aataaaaaga
    33721 agtgttttaa caagagatat agagttagtt aacttgtatt taccagcaca tttaaaaata
    33781 aaataagcta taaataatta aagaaaaagg tgattagatg gaaatagaac aagcaacaat
    33841 acgcctgcct agagaactta aagacaagct tctaaaacag gcaaaagtta aaggatatac
    33901 attaaaagat atgatagttt ttattctaaa ggattatctt caaaatattt ctcaagaata
    33961 aattcaattt ctctacctat agaacgctta tctttttgag caagttgttc gattttctca
    34021 aaaagaagtt tattaattct tagtgtaaat cttttatcct cttcacgagt ataaatatct
    34081 ttattagaca tatttatcat tcctcacata aaatttgacg tctttatgac actttaataa
    34141 taaaataata taaaagaaaa gtcaacaaaa atgcttgacg tcaaatatat gacatggtat
    34201 tattaaaaca agaagggagt tgacgtcaaa tatatgacaa atgaaagagt tagatttaca
    34261 tttagattac cagcaccatt gcttgaaaaa attaaaaata gagcatcaat agaaggtagt
    34321 tcaatgaact cacttatatt acacatactt tgggattaca taaaagaaat agaaaataag
    34381 gaggtcaaat aatggttgaa ttggtaaaag aatttgattt acaaacaatt aaagtaggaa
    34441 atgcagtaaa agtaaattgc aaaagatttg gttttgaaat tgattgtata gtagtagtag
    34501 caacagaaaa agaattaaat ttagcatact ttgatgaagg tagaggctgt atggagtatc
    34561 aagccttaat aacagaagat attcaagatg gtgattatga gattaaaatt ttatcttagg
    34621 aggaaataaa atggctgctt taataatggt aggtttattt gcaatatgtt tagtaggatt
    34681 agttcaaaat agagattaaa ttaaggggga ttaattatgg aaagtttaaa aagaataaaa
    34741 aaaatggttc aaaaacaatt agttttagct gaattagaga taaacaaaaa tagcaaactt
    34801 tatgaggaac ttgaaaataa ggatagaggt ttaatagatg atatacacat gagagaatat
    34861 ctaagagaaa aagttgcatg ggaaagagta aaatatgcta ttgaaaatat tttaggtggt
    34921 ataaatttag agattaaatc aaaggaacat gaagaaagtg aggattacaa gatatttcaa
    34981 ttaattttag aagaacttga aagagataaa cctatagatg ttcagatata agaaaagagc
    35041 cattgccgtg gctcaattca aataaatata tcaaaattta attaagctaa ttatagcaca
    35101 aacggaggga aattatgagt actttatatg aattaactac agatttatta gaaatagaag
    35161 aaggtttaac agaaacaaca ggaaatgaaa ctgaaaaact agaggaaata aaagaaataa
    35221 taaaacaaga gatacaaaat aaaaacacta ggatagtttc agtaatatta aacattgaca
    35281 gtgatataaa ctctatagat tcagagatta aaagactgca agagttaaaa agggtcaaaa
    35341 agaatactct tgatagatta aaaagcaata taaaagactg tatggaatta cttggtacta
    35401 aaaaagtaga aacagtttta ggaaatataa gtataagaaa gtcagcaggt agcttagtca
    35461 tagaagatga agaaaagata cctgctatat ataaaacagt agagcaagtt gtaaaagtag
    35521 ataagaatat cattaaagac tttattaaaa aaggtcatga ggttgaaggt tgcaggattg
    35581 aatatggaac tacactaaca attccaaaag ctaaaaaaga gtaggtgagg accatggaaa
    35641 ctaataatgt ttatataaaa cttgtaaata tacagagtac tttaaaagct cctaaaagtc
    35701 aatttaatag ctttggtaaa tacaactata ggagttgtga ggatatacta gaaggtttaa
    35761 aacctattct aaaggaagaa aaagcattag ttatattgga tgataatatt gttcagatag
    35821 gaaatagatt ttatgtagaa gctacagcaa ctttaataga tgcagaaaca ggagagaaag
    35881 tatctacaaa ggcattagct agagaagatg aaactaaaaa aggtatggat ttagcacaag
    35941 taactggaag tgtatcaagt tatgcaagaa agtacgcttt aaatggatta ttttgtattg
    36001 atgatacaaa agatagtgat gcaacaaata aacatggaaa tgagcagaaa aaaaaagaag
    36061 ttaatgagag tgaattaaat acactatatt cgctaggaga atctatagaa aaagataaaa
    36121 atagagttga tagtgaagta tataagaagt ttggaaagtt agcagtagat ttgactaagc
    36181 aagagtatga gaaagtttta aatggatata agagcatttt agagaagcaa aaacaagagt
    36241 aggtgatagt attggggatt ataagagtaa gcaaagacaa agataatcca tatgtagttt
    36301 taaataaaac ttgtttggaa gatgtaaaat taagctggca agcaaaaggt ttacattcat
    36361 atctgattag taagcccgac cactggaaaa tctatgttaa tgacctttgc aagagaagta
    36421 aaaatggaag agatgctaca gcaaatattt taaaagaact tatagaaaat ggatatataa
    36481 caagaaaacc ttgtagagat tctaatacta ataaaatgct tggagggtat gattatgaag
    36541 tatatgagat accacttgaa aatcctcaga agctaaaatc ccgaaaaact gatttcccgg
    36601 aaaccggatt tcccggaaac cgggtttctc ggaaaccgga aaacacggaa gtagtaagta
    36661 atgactttaa agtaaataat gatattacta ctattgttat taatgaacaa tccaataaag
    36721 acaaaaccac ctatataaaa aaatactttg aaaaatatat aggtgtgatt actcctaata
    36781 actttataga gttaatgagt tacttagatg atggaatgga agctgatgta ataataagag
    36841 ctgttgatga agctgtaggc agtggagtta agaattataa gtatgtaaag acaatcttaa
    36901 ataattggat agaagcaggt gtaaaaactg ttttagaact tacagaatat cagaatgaat
    36961 ttgaaagaaa gaaaaagagt aagcaggaaa agaagcagtc taatagtaaa actgtgaata
    37021 ctcataatgt gaataaaaat aagtttgcta acttcaatca gactttcact caatatgaag
    37081 aaaaagagct agatgagatt attaaaaaga gtcagaagga aaaatttaaa taaaattaaa
    37141 cttctaggaa gtaaatatca atatattgct tcctagaaaa ggggaggtat aaaatggcga
    37201 gaatatatgc acaaagaagt ggttctttaa atgaacaaga tagattggag ttattaagat
    37261 tacttgggaa agctggatat acagtaaagg ttgctagaga gaagcaaaat agcaagacaa
    37321 cttatactta ctttgttgag tatacagaag aacaggaaga aaaatagaag ggggctagtt
    37381 aaatgaatac aataacttta gttggaagat tagttgcaga tgcagaattg aagtaccttc
    37441 caaattcagg tactccaaaa ataacctttt caatggcagt agatagaagg tttaaagata
    37501 aaaatggaaa taaaataact gattttattc aatgcgagca attaggaaaa catgtagaga
    37561 atttagtgca atatcttgtt aaaggtaagc ctatatatgc tgttggagag ttaaatatat
    37621 ataattacaa agatgaaaat ggttgctgga aatctattac taaggttaat gtaaatgctt
    37681 tagaactact ttctagtaaa aatgataata atgctaaaca agaatatgta ccaccaggat
    37741 tagacccaca aggttttcaa gcaatagatg atgacgatat acctttttaa ttaagttaaa
    37801 taatctaggg agtaattatg caatattact tcctaggagt taaaaaatat tggaggttta
    37861 agagtgaaat atgagtgtga gaaagtgttc ttagaatgcg ataagggaag ttttgagata
    37921 aatgatacaa gagctgaaga agtaacattc gagggtacag aaatagacaa tctgtttgag
    37981 agagtaaaat atgaagggac ttttacaata atatctggat gggattcttt attgagagat
    38041 atattgtggc ttgaaatgcg gaaagtctta aaaattataa caggaaaaat gatgatgggg
    38101 gtattaggaa tgataataat tagaagtcaa gataaaactg atttagtaaa agctgattgg
    38161 ataaatgttg ataaagaatg tgtatatgca atgtttggag atatggataa ctttaaaaag
    38221 ataggtgagt atgaggatga aaaaagggct atacaagtgc tggataatat acaaagattt
    38281 attgaaggtg gaactagaac agattctata gacagttata aggttagaag ttatagaaat
    38341 agaatatttc aaatgccagt taaataggga ggttactgaa atgttaaagg ttgaaaaata
    38401 ttttagtggt tctttaggaa caaatatcat agatgatgac cctacatgta gaaattactt
    38461 agcactatat tgttgtgttc acggagtaag aaaaaatgga gaacttatat ttccaacatg
    38521 tgaaaaaatg ttaatagagt ttaatgttga taaaaataga aaaaagaaaa agaaaggttc
    38581 aaaggtaaag ttgattaatg ccaaaactgg tgaagaaaaa atatttgatt ctatagatag
    38641 tgcagcatgt tttttaagac tacagagtca ggcagtttac caaacaatta aaaagaaaac
    38701 taaaacaaga agtggctgga aagctgaata tattgaggag gaataatgga agtttcaagg
    38761 actgaataca caattaaaag agcaaaagag ttatatgata atggagagga tatatttatt
    38821 gctatagata aggctagaga agaatatgag gagatggtta aaagtgaata tcttagctag
    38881 tgtgatatta gtaataggaa gttttatagc tggtagagtt tatgagtata gattgaattt
    38941 aaatgaaaat gatgaagctg attcaaaagt acttttagat gtttttaatg aaattagtgt
    39001 gttaagagaa gaaaataaaa gccttaaaga aaagttacaa gagaaagagt tactattcat
    39061 caatagatta atagattttt tacatgataa aaagatatgc gaatgttgta tttatgactg
    39121 taagattgat gatattgaat atgattgtga agatggtatt aaaaagtggc ttgatagcga
    39181 agaacttata tttgaataga agaaatatct aattaaaaca gtttagagag ttgcaaaatg
    39241 ttttttaata aaattatcat tgagatgttt tgtaactctc aaaaatgaaa ataaggaggc
    39301 gttgtattgc ttacattttt agatttattc gcagggatag gtggctttag gctagggatg
    39361 gaaaaagcag gacataaatg tttgggacat tgcgaatatg ataaattcgc aaatttaagt
    39421 tataatgcca tgcacaaacc gaaggaggat gaatggtttg aaagagatat tagagaaatt
    39481 agaacagaaa atatcccaag agcagatgtc tggtgttttg gattcccatg tcaagacatt
    39541 tctgttgcag ggaaacaatt tggattcaga ggagaacgtt caagtttatt ttttacagtt
    39601 acaaaactta ttagagaact caaagaagaa gatagaccca agtatttact tattgaaaac
    39661 gttaaaaatc tacttagtgt taatggagga tttgatttcc tcaaagttct cgttgaactg
    39721 gatgaaatcg gctatgatgc agagtggcaa gttcttaatt ctaaaaactt cggagtaccc
    39781 caaaatagag aacgaatatt cattgttgga cattttagag gacgaagtac acgaaaagta
    39841 tttcctatcg aaagaaaaag tagaaaaaat cttgagcaac taaataatcc aactcatagt
    39901 acaaatagaa tttatgatgc agttggaatt gctagatgta ttagaagtca ggcaggaggt
    39961 ggaggtgcta aaacaggtct atactttata gacttaaata aaaactctaa agtaacaata
    40021 aatgctagat gccttaaagc aaaatataat gcaggtgtga caaatagaaa ttgtgataat
    40081 agtggagttt tagttaatgc agttttaacg cccgataggg taaataaaag acaaaatggt
    40141 cgtagaatta aagaaagcgg agaaacaatg ttcacattga cagctcaaga taaacatgga
    40201 attttgaaaa atggagatat aagaaggtta acaccaaagg aatgctttag gttgcaagga
    40261 tttccggata aatattacga aagagcagca agtgtatgct cagatagtca actgtacaag
    40321 caagcaggaa atgctgttac tgcaaatgtt gtatatgaaa tagcaaaaag aatgggctaa
    40381 aagttgcaaa atgtctttta gtatgaatat ttttgaagtg ttttgtaact ctcaaaaatg
    40441 aaaataaggg gtgggataaa tgtatgaata tatattaaga tggcaaatag gattatcgtt
    40501 agaaaataga aaaatacatt atacatatgg aagtaaagaa gctttaagaa agaaagcaaa
    40561 ggcattggct aaagatgaaa atatagtact
//
LOCUS       DAMJWS010000028        39773 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMJWS010000028 DAMJWS010000000
VERSION     DAMJWS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.028"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(86..490)
                     /locus_tag="PC399_001575"
     CDS             complement(86..490)
                     /locus_tag="PC399_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2592439.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPLIYLKYKPDK"
     gene            complement(682..1869)
                     /locus_tag="PC399_001576"
     CDS             complement(682..1869)
                     /locus_tag="PC399_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF2592440.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(1901..2326)
                     /locus_tag="PC399_001577"
     CDS             complement(1901..2326)
                     /locus_tag="PC399_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592441.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            2784..3533
                     /locus_tag="PC399_001578"
     CDS             2784..3533
                     /locus_tag="PC399_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2592442.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(3699..4064)
                     /locus_tag="PC399_001579"
     CDS             complement(3699..4064)
                     /locus_tag="PC399_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2592443.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(4080..4856)
                     /locus_tag="PC399_001580"
     CDS             complement(4080..4856)
                     /locus_tag="PC399_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2592444.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(5303..6730)
                     /gene="ascB"
                     /locus_tag="PC399_001581"
     CDS             complement(5303..6730)
                     /gene="ascB"
                     /locus_tag="PC399_001581"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2592445.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(6791..8719)
                     /locus_tag="PC399_001582"
     CDS             complement(6791..8719)
                     /locus_tag="PC399_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2592446.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(8864..9706)
                     /locus_tag="PC399_001583"
     CDS             complement(8864..9706)
                     /locus_tag="PC399_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2592447.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            complement(10053..11423)
                     /locus_tag="PC399_001584"
     CDS             complement(10053..11423)
                     /locus_tag="PC399_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2592448.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQDVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            complement(11462..12133)
                     /gene="pgmB"
                     /locus_tag="PC399_001585"
     CDS             complement(11462..12133)
                     /gene="pgmB"
                     /locus_tag="PC399_001585"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2592449.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            complement(12135..14525)
                     /locus_tag="PC399_001586"
     CDS             complement(12135..14525)
                     /locus_tag="PC399_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2592450.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVEDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSVGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            complement(14551..15999)
                     /locus_tag="PC399_001587"
     CDS             complement(14551..15999)
                     /locus_tag="PC399_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2592451.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMIIGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(16402..17289)
                     /locus_tag="PC399_001588"
     CDS             complement(16402..17289)
                     /locus_tag="PC399_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2592452.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(17389..17838)
                     /locus_tag="PC399_001589"
     CDS             complement(17389..17838)
                     /locus_tag="PC399_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2592453.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            complement(17828..19735)
                     /locus_tag="PC399_001590"
     CDS             complement(17828..19735)
                     /locus_tag="PC399_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2592454.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVSTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(20003..21898)
                     /locus_tag="PC399_001591"
     CDS             complement(20003..21898)
                     /locus_tag="PC399_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2592455.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(21934..22812)
                     /locus_tag="PC399_001592"
     CDS             complement(21934..22812)
                     /locus_tag="PC399_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF2592456.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(23517..24950)
                     /locus_tag="PC399_001593"
     CDS             complement(23517..24950)
                     /locus_tag="PC399_001593"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2592457.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPKGDESTPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     NRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNDSAGKA
                     DVVENPYLKISDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     DYRIEYLASHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            complement(24973..26814)
                     /locus_tag="PC399_001594"
     CDS             complement(24973..26814)
                     /locus_tag="PC399_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2592458.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLSANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDIISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDRIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            complement(26940..27773)
                     /locus_tag="PC399_001595"
     CDS             complement(26940..27773)
                     /locus_tag="PC399_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2592459.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGREELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKNIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            complement(28817..29029)
                     /locus_tag="PC399_001596"
     CDS             complement(28817..29029)
                     /locus_tag="PC399_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592460.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(29030..29446)
                     /locus_tag="PC399_001597"
     CDS             complement(29030..29446)
                     /locus_tag="PC399_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592461.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(29516..29929)
                     /locus_tag="PC399_001598"
     CDS             complement(29516..29929)
                     /locus_tag="PC399_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592462.1"
                     /translation="MSKSELGVKANLVSELMMLSYFFIDLLLQKFIFKGEGIWFTFII
                     LLAFWVCNLMYNKKYNDLVDEASKLMLLKVDQIASKIMLYSMFVIAVLFSVNHTRALD
                     ISNLDIGIMLLAVLFIQSLSKLILYIYFDKKGICN"
     gene            complement(30763..31392)
                     /locus_tag="PC399_001599"
     CDS             complement(30763..31392)
                     /locus_tag="PC399_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF2592463.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(31471..32433)
                     /locus_tag="PC399_001600"
     CDS             complement(31471..32433)
                     /locus_tag="PC399_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2592464.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(33195..36866)
                     /gene="cbpA"
                     /locus_tag="PC399_001601"
     CDS             complement(33195..36866)
                     /gene="cbpA"
                     /locus_tag="PC399_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HDF2592465.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAEGDTNAGNAGGDANAGNAGGDANAGNAGGDANAGNAEED
                     SNAGNAEGDANAGNAEEDANAGNAEEDSNAGNAEEDMNALVVEEATAEDPTSVGDFKI
                     SGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKT
                     ASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLTIDDSVVNRD
                     IEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNT
                     ESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTITAKGAYHGAG
                     IGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTN
                     RGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLE
                     KKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDDQGVLYLWFSDQ
                     FKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQYEEFTIDDTPPYLIIKRY
                     DGLPFEEDVEKLFLDWILKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDTNLG
                     SDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYKAEIIPADTETSLVVEESK
                     AKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSE
                     SHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVD
                     GDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDK
                     NIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNG
                     GNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPD
                     NGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKPN
                     INIDIDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDIDGDGKPDINIDIDGDGK
                     PDINIDTDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQTGDQSNIMLDLALM
                     FISLFFLIKNLTNKYLRRK"
     gene            37135..37707
                     /locus_tag="PC399_001602"
     CDS             37135..37707
                     /locus_tag="PC399_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDF2592466.1"
                     /translation="MEKLVKILINIICIIALTFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNINYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNNNDEFKYSLNYIT"
     gene            complement(38707..>39773)
                     /locus_tag="PC399_001603"
     CDS             complement(38707..>39773)
                     /locus_tag="PC399_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2592467.1"
                     /translation="IIKKKKDICDYLNKISNYKKVKSTIEKLETRGKMPKWYLELINK
                     VKNILENMLYNRNLSDEQIFYLNLFLDIQNLFLGIYELKSESVIFENTLDDNKIIAKM
                     DTYIIIDYINASFEYLGYVLNGSALNSIDYGVPQNRERFVLIGAKKDFLKNKKIETPK
                     PIVGENYVTVRDAIGDLAEYEASKGSMDYCFEKNSNNIEKDFYRKIILDSNKIYNHVC
                     TDTRNIALKRFEYINQGNNFHSLPDELKGTYADPERTQNTIYKRLVYDKPSDTVVNVR
                     KSMWIHPIKNRAVSAREAARLQSFPDSYKFLGTKDSVYQQIGNAVPPLLGRVVAEKIL
                     NLFNDEPNEYLRDVFEALDG"
BASE COUNT        12892 a         6994 c         4131 g        15756 t
ORIGIN      
        1 taaatactta aatttagttt tatttaaacg ctctagtgat atttttaaat aaattttaat
       61 aaaaatcatt ctattttgtc ataaatcatt tatctggctt atatttaaga tatattagtg
      121 gatactttac actttttaat tcctcattac ttcttaaaag tttattatta ttttccatat
      181 cataaatttg taacattact ttatttacat atagagcata ataattatca ctataaaaat
      241 attgatactc atatcgaggg tcatctttgt aatacacttt gtatagtatt ccttcgtaac
      301 ttgccttctt ggctttttct tttgtaatac tttttatatt atcttttgaa atatcttgtt
      361 ttaccatata cttatcaata tatgtttcag catctttcca tggtttaatg cagtttttat
      421 atacaacaaa ccctgttaca cttataatac ctataattaa tactataaaa ataactttat
      481 attttttcat attaatcctc cttcatttat tatttaaaac tcgatataat gcactaaaaa
      541 aaggatgtta tcttttttat taaaatataa catccctcat atttagcatc aatatatatg
      601 ccccaactgg taggttaaaa gaccccactg tcagtacttt gattaaatta catgtatgta
      661 tatatatttt taacaaatct attatatgtc aacttgtcta ctattacata taatctgtaa
      721 tattacatct gctgcttttt ttgagcctcc tgcattataa aagctttttg atagtttagt
      781 tgcattttct ttatattcat tgtcatttat aacttgaaat actgcttttt taatactttc
      841 tgacttatcc tcttttaaca taattcctgc tcccaaatga acaactctct tagctaccat
      901 tctctgctcc gaatgttgag gaaataatac cataggtact ccataatata aactttcatt
      961 aacactattc atcccagaat gagtaacaaa cacatcagtt ttttgaagta tagcaatctg
     1021 ttcaacacta tttttgacct caaaattatt tggtatattt cctaaacttt taatatcagt
     1081 acttctacca acagacataa tcacatcaac atcagaatct ttaaatgcac ttatacagtt
     1141 tttataaaag ctaacatttt tattattaat tgtaccaagc gaaatatata ttagctttct
     1201 ttttctctct ttaggttcaa taatcaactt tgatactgat ggacctacaa atgaatactt
     1261 actagagaag gtttctacca ttggttgaaa ttcttttgat gtatatacaa tggtatccgt
     1321 atcattatca ttagaaacta tagaaataaa attttttact tcataccctt ttctttgtaa
     1381 aagctttatt tttttattaa tgcgtcttac accaaaaatc atacgtataa tttctataaa
     1441 gttttgtttc atcaactttg ctgtatgttt attaaatgca aaagttgttg tagagcatac
     1501 atatggaata ttaagttttt gtgcaaataa ctttccccaa aaacttaatg aatctgatac
     1561 aatgcaatct ggattaaaat ctttaagttc tctacaaact ttttcatcta aagaaatagt
     1621 agtatctact atcatctcaa ttaatccagg aaaatcctta ccaatttttt tctcatctcc
     1681 agggagtaat tctggaaggt atttatcaca acaaataaat tttgctccag caccttcaat
     1741 tttatcttta aattcattaa aagaataata caatacctca tttcctctat ccactaattc
     1801 acgaactact tcaatagtag gattagtatg accatatgca ggaatgctaa aaaatactat
     1861 tttactcata tttcatacct ccttttataa tttttttaaa tcattcttct ttacgtacta
     1921 ctcccacatg acctataaaa tttatcaaat cacgttgatg aacaattcct aatcctcttt
     1981 cctcatctcc aagtaccaat aatttatctc ctgcatttat attaaatact tctcttgctt
     2041 cttttggaat gacaatctga ccacgttcac caactacaac tgctccaaag aaaaactttc
     2101 ctttaggagg tactataata cctgtttttt cttctgaatg attaactaag tcatctagtt
     2161 tgacattgta caattttgca agtttaatgc aactacatat atcaggaata gattctccac
     2221 tttcccattt tgctattgat tgtctagaaa cattaagttt ttcagctaat tcttcttgtg
     2281 tatactggtt gatttttctt agacttttta aattctcatt aatcatattt ttcatacctc
     2341 ctatgtttat aatattggat ttattattta aattctatca actaatctta acattcatat
     2401 atatatttat atacttttat tatataaatt tattattcag gtaacatatt tccttaagtt
     2461 atcgtaaaca aaaaaacaag cacaaatcta ctaactgttg ttaaagtatt taagttaagg
     2521 tcatttacat tatcaaatat ttctacacct ttaaaataac ctttatattt ataatctttc
     2581 ataagcaaat tattatccct tctcatatac aagtccagtt gatgtcttgt tgctaaattt
     2641 aatggtaatc aaattcttta ccaataagta taaaaattac taatgtgtta taattttctt
     2701 aactactaaa ttttacatac atttgaatag aatatatcaa taaattacta taataaataa
     2761 aaaatagtta gggagatatt attatgattt tatattttag cggaactggt aatagccgat
     2821 atgtggctcg taaaattgca caagaattaa atgatgaact gatatcacta aatcaactta
     2881 taaaagagga aaaaacagat gaattaatat cagcagataa accattcata tttgtatgtc
     2941 ctacttatgc atggagattg ccaatagtag ttacggattt tataaaagaa acaaaatttc
     3001 taggaagcaa cagagtttac tttgtaatga cttgtggtgg agatacagca aaatccataa
     3061 actatattca aaaactatgt aaatacaagg aatggcaatt aaaaggtatg gcagaaatta
     3121 aaatgccaga gaattatata gctttatttt ctacaacaga caaagaaaca tcaaaacaaa
     3181 tgattaaaga agcagataaa caaatatata ggattatttc tgatatacgc aatgaaaatg
     3241 aatttgaaac tattactcct agtggcttag gaggtacaat taaaagtggt ataataaata
     3301 cagtttttta taagacaatt attagtgcca aaggatttca ttatactgat aaatgcattg
     3361 gctgtggaaa atgtgtagag ctatgtcctt taaataatat taatttaaaa aacaaaaaac
     3421 cagtttggaa aaataactgt actcattgta tggcatgtat ttgtggttgc cccactgaag
     3481 ctattgagta taaaaataaa actcaaaaca gagaaagata ctatttagaa taatatattc
     3541 ttaatattta aaccattata aaatgaacta tacccattgt tttatacagt tttacatatt
     3601 ttaaccgtct cctaaaatgg gtatagttca aaagatagtg tctcaaagtg aattttttag
     3661 ttcctaaatc aatctttttg tattaaatca agtataattt aatcaccttt tcttatatca
     3721 ttttcactaa tatgttgtat tccaacaaat ataagattta ttagctctgt tagtgacttc
     3781 cattgtgatt tttttgaatc aagataataa taatttttcg ttccttctct acgcaaatta
     3841 acaatctctg cttcttttaa tatcttaaga tgatgagata cagcaggacg agaaagatgt
     3901 gttttttttg tgatttctcc cactcgtata ccattaaaat cactctctaa aagagcaatt
     3961 aaaatcaatt gtctagtctc atcacccata gcagagatag ctttgcgaca ttctttaaat
     4021 ttagacgtta tttcttgcat tctttgttga cattcttttt ccataaaatc caccctctct
     4081 tataagtatc taacagttgc tacttctttc tgtgcagtac gtctatattt aacatctggg
     4141 tatccaatta ctaatgttgt aagaacatga ttactacgtt ttagacctaa aagctttttt
     4201 aattttggcg aattatttgc tacatctgaa aaatatccac taaataaaac acctagacca
     4261 taagactctg ccattaatgc catatttgat gctgcaagtg aaccactaat cttatctttt
     4321 gtaattatca ttattgcaat aggagcatgc ttaaagaaaa agttatcgtc aatctcaact
     4381 tcttttgaat atttaatagc tatatttgca ataggtttta tttttctaaa aaatttcact
     4441 gcttcgtttt cataaattga ctttttattg tctaaaacaa tatatgatac atcttgtgaa
     4501 tttttagcac taggggtata cctacctgcc tcaataattt gctttataat ttctgaagaa
     4561 acttctttat ctttaaattt acgtatactt cttctagatt taattgccat tagtaattca
     4621 tctgaatcta gctttggctt atttgtaagt tctataggag gttcatcaaa tcctgtcaag
     4681 gtaatagctt ttgtgggaca tattgctgca caatgtccac acattagaca atcttgtttt
     4741 ttaattactg actttttatt ttctataatt atattgttta ctggacaatc atttttacaa
     4801 agcccacatc caatgcaaag ttctttgtca acttctatta tatgttggta tttcatattc
     4861 attcatctac ctttcgttta ttagtttaaa ctattataac acgtagacat tttttatatt
     4921 cattggtaaa gaatttgatt accattaaat ttttaatttc tctattaaat aaatttttca
     4981 tagaatttta aaatatcata gtttatacat aataaaaaag acaagcttaa aacctaagct
     5041 tgcctttttc atcttttatt acaaagtaaa ttgcacttac ttcccatact tcttaacaat
     5101 tttattttct aaaattttat ttgtgaatca tactattgca tcaatacaat gtaaaataat
     5161 tctatattga ttttctatag ttaaaaaagt taaaaattat acttattatt aatttttata
     5221 ctcttcttat ttaatacaaa gttcttattt aacatagatt tcttgtctaa tatagatttc
     5281 ttatttaaca tagatttctt gtctaatata aatcttcacc atttgaagca attactttct
     5341 tataccattc aaaacttttc tttttcattc ttcttagact tccattacca ctattgtctc
     5401 tatctacata tataaatcca taacgctttt ccatttcacc tgtaccagca cttactaaat
     5461 caataggtgc ccatgtagta taacctaata aatcgacccc atctatagaa actgcatcat
     5521 gcatagcttt tatatgttct cttagataat caattctgta atcatcttct acataaccat
     5581 tttcatcagc tatatcttta gcacctaatc cattttctac aataaatagt ggcttttcat
     5641 atctatcata tacatgattt agtgtaattc tcaaacctaa tgggtcaatt ggccatcccc
     5701 attgactgta tgaaagatat ggatttttag cagatgtaaa tatgtttcct tcagccatat
     5761 catctgcatt ttcatcggtt ctaacacaac ggctattgta atatgaaaat gaaataaaat
     5821 ctactgtata tttattcaat aattcaatat caccaggttc catttctggt gtaatacctt
     5881 tttgctccat atactttaac gcatagttag gatatttacc tttagattgt acatccacaa
     5941 agaagtaatt ttctctatct aactttgtag cttcccatac atcattagga ttacaactat
     6001 aaggatatac acttccagct gcaaacatac atccaattaa gttatttgaa tcaacttcat
     6061 gtgctatttt agttgcaatt gcactagcta caagctcatg atgggcagct tgatatttta
     6121 tcttttcaac atcttcacct tcttcaaagc aaataccagc acccataaat ggagcatgta
     6181 aaatcatatt tatttcattg aaagttagcc agtacttcac taatccttta tatcttctaa
     6241 agataacatt gcataatttc tcatagaaac ctatcatttt tcgattacgc catgcaccat
     6301 actttttaac taaatgcatt ggacaatcaa agtgtgtaat agtgactaaa ggctctattt
     6361 tatatttttt acactcctta aatatatttt cataaaattg aagtcccttt tcatttggtg
     6421 tttcttcatc accttttgga aatattcgac tccatgcaat gctcattcta tatactttaa
     6481 atcccatttc agcaaataac tttatatctt ctttataatt atgatacata tcaataccag
     6541 ttttagctgg ataataatgc tcatcatcaa aatcaaacat ttttagtttc cctgtaataa
     6601 ctgctgttct atcttttcct gttggacata catctacatt tgccaatcct ctttcgtctt
     6661 cattgtatcc accttcacat tgattggccg cagtagctcc tccccataaa aaatcattct
     6721 taaaactcat agtcaaagcc tcctaaaatt ataaccaaag cactagatta tgctaatacc
     6781 tcaatcttta ctaaaataag actgtaatta gattgtcttc taattcaata tttttacttt
     6841 ctgtttctac tacatctaaa aattgtgatg aatttgtaat aacaattgga gttacagtac
     6901 tataccccaa ctcttttatt gcataaacat caaagtttaa tatagtttga ccttttgtaa
     6961 ttctatcacc ttgttttata catgcctcaa aacctcttcc ctctaattga atagtattta
     7021 atccaatatg aataagcact tcaacacctg tatcagaaag aatccctata gcatgtagac
     7081 taggaaataa ggttgtaact accccatcta ctggagcaac tacttttcca tcactaggta
     7141 ttattgccac cccttgacct agtacaccag atgcaaatgc tgcatcctct atatcactta
     7201 attttaatac ttcacctttt ataggacttg ttactatttc tctttctaat ttagtagact
     7261 tgacttcttt tatttcttct ttacccttat ttcttacttt tttagactct tctactttct
     7321 catctttata taaaatcata gttaatacaa aagctacgac cattgatata gctataccag
     7381 aaagagctac tattaagtta cccattcccc catctggttc aatcattgct ggaagttcaa
     7441 atattcccat tcctccagtt ataaattttc taagattaaa gaatccatag aaagcgcctc
     7501 caataccact agcaatacaa cttattataa atggtttctt taaaggcaat aatattccat
     7561 aaattgctgg ctctgtaact ccaaatatac cagatataaa gtttggtatt gccatctctt
     7621 ttagtttttt atctttagtc ttgaaaaata ttgccaacac aactgccgaa cttgcaaatg
     7681 tacaggcaaa gaatggcatc ataacattat catatcctaa agtcataaca ttgtttatat
     7741 atacaggaat aaatccccag tgcataccaa aaataacaag aatttgccaa gtaaatccta
     7801 ctatagcacc tgccaatagt ggactaaaat ctcttattga aattacaatt tgtgaaataa
     7861 gtgtagaacc aaatgttgct atagggccta taactataag tgcaacaggc actgttatta
     7921 ataatgtaag cattggcaca aagaaaaact taactaaatt tggaacacac ttattaaata
     7981 atttttcaca cctagaagca aaatacacta taaatataat tggtacgact gttccagtat
     8041 aatcaattga tacaattggt agtccaaaga aatcaatata tactggtgat gcaaacattg
     8101 ttcctttaaa taaagtgtac atcacatctg ctcctgctga tatactgcta ccttgtatag
     8161 caggataaca tactgcagct cctagtgcta atcccaacat tggttttaat ccaaacttct
     8221 tagctgaagt atatcctaag aacaaaggaa ggaatgtaaa taatgcatct cctgctgcat
     8281 ttattaccat ataaccacca gatacatcac tatataatcc cattgctaca aataatgcat
     8341 taaatccttt caacatacca caagctgcca ttattcctaa cactggctga aaaattcctg
     8401 atattatatc tataccttta tctagtatat tacctgattt tttagtttct gaacttgtac
     8461 ttgaatcatc taaaccaatt atttcaacta catctttata gacagcttca acatgatttc
     8521 ctataactac ttgatattgt cctccacttt tcattacagt aacaatacct tccatgtttt
     8581 ttaatacatt gtcatttgct atactttcat cttttaaagt aaaacgtaat cttgtaatac
     8641 agtgtactaa cccactaata ttttcttttc caccaacatt gtttactatc tcttgtgcta
     8701 attgtttgta ttttcccatt tttctcctcc atatttcgtt taaattaatt ttttgaaatt
     8761 tgaaggtttg gccaactcaa tgttaccatc caaccataag tataattttg ttagtatcac
     8821 tcctttcaat cattcttaat atactactga aaaattattt cacttacata tttgagtctt
     8881 taactacttt agctaaatgt attgttaaat atataagctc atcatctgtt aatatgtagt
     8941 tatattgttt tttaatgaaa tttttaattt taactacaca ttcaaactct ttattatatt
     9001 tagatttaat aatgcttagt aattctttgt ctgtctcacc ttcatgtgca ctatctaaaa
     9061 gcaatctttg cgcaaaaaac tttaaatgca ttataaatct ataataaaac actgattctt
     9121 catgaaattc tatcctaaaa tgatacctaa ctatgctaag tacttcttgt attaacttcg
     9181 taatatcaga tgccaaagta tgaccttcac ttaactgagc attcactata tgaaaggcta
     9241 taaatccagc ctcatcttct ggtaatttta tacctgtttt gttttcaata atatctagtg
     9301 ctttcattcc aatttcaaac tcttctttat aaaatctttt aatttcccaa aggattgcat
     9361 tttttacatt tattccttct ttcatccttt gaattgcaga atatgtatgg tcacttaagg
     9421 atatgtaaat actttcattt agcttcttgt ttaactttat ttgtgcgtat tctataattt
     9481 cacttgataa tttcatgtgt tctataggaa tattattgag taattcttgt agtttgtttg
     9541 atatttcttt atttgatatt ctaaatactt tatttacttt agttttatct attagattgc
     9601 cttttgattt ttgataacca attcctttac ccataacgat aacttcttca tcattatcat
     9661 ctaatgtgat tacaacatta ttgtttaata tcttattaat tatcatgatt atcctccttt
     9721 taagcataaa aaaagcttat ccataataat gggcatactc cttcctagga tactgctatt
     9781 attaatgaat aagcttagct tgtacttaaa caattactac ctaattccaa ttaatatgat
     9841 aaaacatcag acatattttg tcaacttttt atttccatat acttcatcaa tcttttatgt
     9901 tgctttttgt cagttctaat aattattctt attaatatta acttatttct ttgcatcaat
     9961 ataaatacaa aacaagttat agtcaataat aataaaaatt ctattaatgt aaatagttaa
    10021 tattatactt cctctactac attatctgta ctttatttaa catttttttc aactccaaaa
    10081 acatatgtca taataaatgg agcaaccaat gctataaata taactataag tccatttact
    10141 atatttgaaa tccccttttc ccctgataat ccaagtactg ccattatact attttgtgga
    10201 acatataaag tcacatgagt aattcctgca aataaccctg cgattcctgc tcctatagat
    10261 gctaacatag caggtttttt aagtttcaat gtaactccat aaagagctgg ttcagttata
    10321 cccatgatag ctgatatcca tgcagctgat gctaactgtt tcatcttact atctttgctt
    10381 cttaatgcta cagctaatgt tgcgccacct tgcgctaaat tagcacaaac ttgagatatt
    10441 aatattagcg attcttgtcc tgtagttgca aatgctgata tgaatatagg agtcaatgca
    10501 tgatgcatcc ctgtcataac gataaatggc attgcagctg ctaataaaca aaccattata
    10561 aatcctaacc ttccttgtag taaatttatt atactagcta atccctctcc taagaaattt
    10621 cctattggtc caattactat taatgctatt ggcgcactta ttaataatac taaagttgga
    10681 tttaaaattg tttttaacat gctaggtgtt aatttatcaa tgaatttttc tatatatgac
    10741 atcatccata ctgttaaaag tgcagggata acagatgctg catatataac atcttgaatt
    10801 ggtactccta taaatgatat agggtcccca cttgataccc atgcagagaa atttggatgt
    10861 aaaaacacac ctactactat aactgctatt gatgtattaa cattaaattt tttagctgca
    10921 tttattgcaa gtattatagg cataaagtaa aatgctacat ctcctattat atctaatact
    10981 ctatatgtac ttgaatttga tgatagccaa ttaaaagtgg tgcaaagaga taatataact
    11041 tttatcatcc cagatgctgt aagggctggt atcataggtg tcatacaacc agttattgta
    11101 tctattatat tatttaaaat actatttttt ttatttttat tttttatatc gcttttaata
    11161 tctcctgtgg tatttatccc cttatttatt attgcattaa atacaacacc tacctcatta
    11221 cctattatga cctgatattg agttgaagtt gagtttactc caactacctt atctatagat
    11281 tttaatttat ctgcattgac tttcttttca tcttttaaga tgaaccttaa tcgtgtcata
    11341 caatgagtta agtcaattat attatccttt cctccaagtc catcaattat caattgagct
    11401 acttcttcat atttattctt cataccttat cctctctttt aaaataattt ttaatataat
    11461 tttaatccaa attaatcttt ttactccaag cttgtaatag tgtattgtaa tttaataagc
    11521 tagtatcttc tacaatatgg tcacattttc ctagtacttc ttttttcccc acaccaattg
    11581 atgtcatatt agcagcattt atagctttaa tacctgaata agaatcttct attcctacac
    11641 aatcatattg ttttaaattt agaatcttgc tcccttcaat aaaaatatca ggatatggtt
    11701 ttcccctttt taataaagat ggatttacta tacaatcaaa ttcatctttt attcccaaag
    11761 attctaatat cctaggtgca ttcttactag cagatgctat tcccaatttc acattatttt
    11821 ttttaagttc ttttataagc tcatttatcc ctggtaatat atctttatat gttaatgagt
    11881 ttattaactt tatataaatg ttattttttt cttcacacac ctttaacttt tcctctttag
    11941 tataactatc tactttatta gcagataaca atattttatc taatgattct tccctgctta
    12001 tgccttttaa tttttcatta aaagtttcat ctatttctat atctagcgat tttgctatct
    12061 catgccaagc ttcaaaatga tacttagcag tatctgttat aactccatca agatcaaata
    12121 agactgcttt catattaatt actcccttta aaacaattat taacttctat tatgttcttt
    12181 gataatttta tttctttatt atcatgtaat atggttatat ttctaccttt gattaactta
    12241 taataagatt tatattcttc tatatttaca gaaactaaat tatctctaaa taatatatta
    12301 aatttaatat ttttccagtg tttaggtatc cttggttcaa aacttaattt atcatttttt
    12361 attttcattc ctgcaaatcc atatactaaa ctcatccatg ttccacccat attagcagca
    12421 tgtattccat cacttgtatt tttttgtagg ttattaatat ccattaatgc tgtgtccata
    12481 aaatagttat aagctttttc atgatttcca atttcacttg ccatcattcc aaatattgac
    12541 cttgacaaag atgaatcatg agtagttatt ttttcatagt attcaaaatc tctcttcttt
    12601 tgttctaagc taaactcatc agggaaaagc aacgctgaaa gtacagtatc tgcttgttta
    12661 tttacttgat atctatatat tgttaatgga tgataatgaa gaagtaatgg atatttttct
    12721 tttggtgtat ttttaaaatc ccatactgat ttttctaaaa atgaatcgtc ttgtttgctt
    12781 attttcttct cttcatcata tggtaaatac atattttgag cggcatttat ccagtttttc
    12841 acttcatatt cttgtaattt aacttttcta aatacttcat ttgaaaataa attatgctta
    12901 aatttatcta ccatctgtac tgcatatatt aaattatact tagccattaa attagtgtag
    12961 taattattgt ttactattgc tgtatattca tctggtcctg ttactccatt tatacagaat
    13021 ttattatttt ttgattctat ataatctcca aattcaaccc aaaatcttgc tgtttctata
    13081 agaatttcta agccattttg catcataaat tcttcatcat tagttgcttc aaaatacgtt
    13141 ctaataccat aagctatatc tgcatttata tgaaactgtg ctgttcctgc tggataatat
    13201 gcactgcatt cattaccatt tatagttctc catgaatata agcaaccttt atttattcca
    13261 agttctctag ctctttctct tgatgcatct aagattgagt atctataatc taataaagct
    13321 ctggcaatgg atggttgagt atatataaaa aatggtaata tatacatttc tgtatcccag
    13381 aaataatgcc cctcatagcc atcacctgta agaccttttg ctgatatatt agtctttcca
    13441 tctcttccta ctgattgata aatatgaaat aaattggttt ttatccccag ttgaagagtt
    13501 ttatcgcctt caatctctac atcagaggtt gtccaaaact cattcatttt ctttctctga
    13561 atatttttga ctttatcaaa tccaatatta catgcttctc ttaaaaccaa ttctaatgat
    13621 tttaaatgtg cttctgattc taaacatttt aacgactcca gacattctaa tgattttaaa
    13681 cgttcttcat ttagatttaa atcagaatat actctactat agcctacaac aatttctaaa
    13741 tttaaccttt catctttttt ggcatagact tctatttctc gctcaataca ttcatctgaa
    13801 acattatagg tctcatttgt aatatttcca caagtaatat atgaatcaaa cccacaccat
    13861 aaccctaatc tacttctttc tgtaactaaa ttcataaaat acttattgtt atattttttt
    13921 acattaattt taaattgctt cttatttata gatgccactc ttgggtcctc tactttttca
    13981 caattaaact tctccatact attttccatt ttatttttaa attttatttt tccattaaaa
    14041 ttcaatggtg ttattgtata tgaaatcatc attaattctt ctatatcttg tgatactaat
    14101 ctttctatag atactttaac ttttctacca cgagagtttt cccatataaa tgaacgttta
    14161 agaatccctt catttaaatc tagtatccta gaatgttcac ttgtaatacc ttttgataaa
    14221 tcaaagcaat ctccttcaat agagaaattt actatatgag cattaggtaa cttacagata
    14281 gtctgatttt gttttgcata gccataagcg ctttctccat atgtaattgg agattcttca
    14341 taaaatccat ttacatatgt acccatatta tctacatact cattattaaa atcagactct
    14401 tcaatattcc ctctaactcc taaatgtcca ttagctagtg taaacagcgt ttcattggat
    14461 tttattgtat ccttactaaa cccttgctct ataagttggt ccttagtagt aaatattaaa
    14521 ctcataataa cactcctttc ttaaaatgaa ttatgcatga gtttccatgt cattaaataa
    14581 aatttctcca tctgtttcta ttactttttt ataccaaaaa gcactttgtt ttttagttct
    14641 tttaaatgag cctcttccat agtcatcaga atcaacatga attatgccat atcgttttgt
    14701 catctttcct tcacttacac tggttacatc tatacatccc cacataagat atcctataag
    14761 ctctattcca tcttcattta ctccaataat catattttta atatgttctt tcatgtaatc
    14821 tatacgataa tcatcaatta tttttccatc ttcatttaca atatcttgag tacccagtcc
    14881 attttctact ataaataaag gtaactgata cctttcatat aactgattca tagttattct
    14941 taaccctaca gggtcgtttt gccatacact atttgatttt ggaagatatg gattttttat
    15001 ggtttctggt ccgtttgcct ttgatttttc taaaccttca gctctagctg aactacaagc
    15061 actagaataa tagctaaaag atacaaaatc tacagtatta tccttaagtg ttgataaatc
    15121 ttcctctttt attattaatt ttatttgatt tgattttaaa taataattca taaagcttgg
    15181 atacacaccc ctaacttgaa catcagaaaa aaacaaatta tgcctttcat gctctaaagt
    15241 tgcaaataca tcttctggtt tacatgtgta tggatatgtt tttccagcag ctaacataca
    15301 cccaaactta aattctttat ttatatacct tcctatttct atagtttttg cattagctaa
    15361 taattgatgg tgtgctgctt gatatttttt ctgaagttga ttttcatcag ctataaaagt
    15421 taacccagca cctatatatg gaatatgcat tatcatattc atttcgttaa aaggaatcca
    15481 gtatttgaca tcatctttat atcttttcat caaaatttca gcatatttta catataaatt
    15541 aaccactctt ctatcagccc acccagaata tttttcaact agagaataag gtatatcaaa
    15601 gtggcttatc gttattaatg gctctatatt atattttttt aattctttta ttatattatc
    15661 ataaagttgt aatccctttt catttggttc actttcttct cctgttggga atattcttga
    15721 ccaagatata gaaaatctaa aacatttaaa acctatttct gctaacattg ctatatcttc
    15781 tttgtaattt tcacaaaatt taatccctat atgacttgga tagtattgat tattttcatt
    15841 taaaatatta tcctcatcta aacatacatc ttttaatctc ttatcattta atgggagttt
    15901 atcaaactga gataaacctt ttcctccatc taaataaaat ccttcacttt gattggctga
    15961 aattgcccct ccccataaaa aatctttccc taatttcatg catctacctc tttcacaata
    16021 taaagtttta taatttctat ctcatattta gttataagat ttttttcata ctataccttt
    16081 taaataattc aatttaaaaa tccaattata ttttttactc actataatta atattgtcta
    16141 ttttcaatac tttcatatta aatatcaata ttagatatct atgtatataa ttatatatat
    16201 agcaaataca tttttatatc ctcattattg ttatcttatt ctcatatatt gctgtatatt
    16261 tatatataaa aggatttatt tcgaaataga ggttgtattg agaactaaaa tttagacttt
    16321 ataaatgaat ggaattttca ttgttgaaat actgaaaata tatttgattt catataaaag
    16381 aaagtgcctc atgaactaag ttcattttga gacactccct tttttttcca agctattata
    16441 tcttttttta aattctaatg gagagactcc aattttttct ttaaatactc tgtaaaaagt
    16501 ttttgtactt ccaaaaccaa cttcttcaac caaatctaca atggatatat tgttattaag
    16561 taatatccac tttgcttttt ctaatctata ttcatttaaa aaagttataa atgtttttcc
    16621 tgtatttttt ttgaaaaatt tagtaaaata atatacacta aatcctactt cataagacac
    16681 atcttctaat tttataggtt gtttataatt tttttctaca tatttgaata tattgttcaa
    16741 tctctctaat atctctttag agtttatttc atattctact gtattttctt caatttctct
    16801 aggaatctgt ctatataata taggaattaa attatacatt ttacctttta ttgcataact
    16861 atatccttct attttgtata ggtcttcatt gtagatatct atcaatatat cgaaaactga
    16921 ctttcttatt tttgctgacc aatctctact acattttttt atactactga ataaattaat
    16981 taagctttct ttttcctcat ttaataaaat taaatcatta aaaaaagata tatcaaattg
    17041 aaatactaat ctttcacttc ccggtgatgg cagtatataa tgaatatctc ctccttttat
    17101 tatcactatc tcgccttttt ttaattgcat aggcttgtca tttataaata gatttactac
    17161 accttctgtt ataagtaata tttctatttc cttatgccag tgtggtgttg ttaatacttc
    17221 tccttcattt gatagactac gaaaaggaaa ttcatgtatt aatttaggca tttctaaata
    17281 tattgccata tatatcctct cctctctagt gtctaaaaat taatttatac tatttaaaag
    17341 aatacacatt ttattttaaa aacctcaaat ttataattta tacaatacct acacttcata
    17401 aaatatatct aatatttctt tttttgtatt tgcatttaaa agtctttcac ctatattttt
    17461 accacctaaa atgtaagcta ttttagatat taatatcata tcttcattgc ctccagattg
    17521 tggcatagca aacataatta ttacctttac aggtaagcca tctatacttg gccaatctac
    17581 acctttttta gatttaccta ttactatgga tgccttcttt acatattttg tttttccatg
    17641 tggcatggcc atttgataac ctatgctagt agagcctatt ttttctctat gttttaatgc
    17701 ctttataaac ttacttttta aagttatact tcctgtatcg tataatttat ctactaattc
    17761 ctgtattatg ccttcttttg tagttgattt caaatctaaa ataatgcttt cttctgttaa
    17821 tatttctcta atttccattt gttttctcca atagattatt cttaaataat attttaatat
    17881 cctctttgct cttgcaatct attatatagt ttatatagtt cttttgccta tataaatttg
    17941 atatttccat taaaatattc tttaaaaaac tttgattact aatttctaaa taaatatcaa
    18001 tcgttctagt aacattattg atattcatta tccctttatt taatttactt tgcttatgta
    18061 tatatatatt taaatctttt cctaatgaaa tagattcata tttactccct aaattcacac
    18121 ctattaaact ttccatctga gctacatcaa aattaaaata tatattatct tctaaataca
    18181 ttcctaataa gtttgtatct aagttcatac tttttttagg tttagaaagt aaagcatttt
    18241 ttatattatt aatatcattt tccattaata gtactgatac aactatatgt aatttatctt
    18301 tcaaatctaa gtttacagta gatattacaa agtctacatc atctaaattt cgcttactca
    18361 actgatgcat aggaactata tcaactatag tccattcagg aaattctctt tttaatctgg
    18421 ctgcaaggag ctgagatgtt ccatatcctg tatgacaaac tactattact cttttactac
    18481 tcatatttct ttctattgcc gcttgaaaat atgtggcaag atacgaaact tcatctatac
    18541 taatattctt cagattatca tactctgtca taatatttat agtcatcata cataatccca
    18601 gcacatctga aaatcgctct tgtatctctc ctaacaacgg gttttttatt tggatatcat
    18661 atcttaatct gtttaacata ggccttacat gagatgctag acttttctgt agtaagtcat
    18721 catttttgag atttatattt aaaaatcgtg acatattctc tatcattata gatgaaactt
    18781 tttcactttc acttaatcta tctgattcat cattgtaact actattattt tcgctactaa
    18841 atccagatga taccaaaaaa atatatatgt acataatttc atctttcgtt atttttactc
    18901 tgtatctatt ttctatttta tctataagtg tactgacatt tttatatact aactcgtcca
    18961 aattatctac agaaatttct ctttcttcct gagattctat ttgattacct tcttcgattc
    19021 tctttaaaca tataagtata tgggtaatta aatttatata gtagggctga tttatagtat
    19081 atcctaaatt gtattctaat tctgtaatta tagtttctac aaatataata gaatcaatat
    19141 caaataaatt tatcaatgca ctaaatgtag cagaatctaa tcttgttaac tcagatatat
    19201 catgttgttc ataattttca tctaataaat catccatcat tttggcaatc gatttcctta
    19261 tatccacttc tttacctata atcttagttc cttctaaagt tttattaagt tttaaattgt
    19321 attcttttat ccatgcctct atatatttaa aatcatttac tatagaagtt ttacttacat
    19381 aatatttatc tgctaactga tttattgaag ttatattatt tgaatttagt aataaatact
    19441 ttaaaatctc cattcttctc tgctctactg ataaagattt attttccttc gattgtatat
    19501 ttaaactatt aattaagtct atattcttct ttgctaaatc ttctattaat attccatagc
    19561 ctctttttat atcaattttt atattaaaag tttctaaata tttctcaatc atcttcaaat
    19621 ctttttgtaa agttttatca gatacattta atttatcttt aaagtatttt ataggtctat
    19681 atttattttc gtttagtatc aatttaagaa attcaatttg cctcgacaca agcatactta
    19741 gccctcccta cctatgttta tatatttcta tgtttatatt aagtactatt ttaacatatt
    19801 tactaactat gtaaaattac taagatactt ctgtatcata aaagaattta tgatacacct
    19861 tactaaattg atataataga taatataatt tcaaaataaa aattaaggat atttagaagt
    19921 agttaactga ttctaaatat ccttattatt attctatata cttgttgtta ttccattaat
    19981 tatttttact attatttata atttaagcac caagtaattc aacttcacta ttttcactcc
    20041 ttatagtagg ctttgttacc cctataatca atgctgttat tactgtacca atcaatatac
    20101 aagctatata tgcaactggt gcactacatg caaatggtat tacaaatatt cccccatgtg
    20161 gtgccattaa agttatatct aaactcatac atattgcccc acctatacaa gaaccaatta
    20221 tatttgctgg aatcattctt aatgggtctg cagctgcaaa cggtattgcg ccttctgtta
    20281 tgaaagatgc ccccattata gcagttggta gagatgactt tctttcttct ttagtaaact
    20341 tatttttgaa tattaacata gctaatgata ttgctaatgg aggtaccatt cctcctacca
    20401 ttaatgctgc tgatggttca aatatattac ttgaaaacat agccaatcca aaagctgaag
    20461 ctgttttatt aataggtcct cccatatctg atgccatcat ccctgctaat attgcaccta
    20521 ataatacttt attagttcca gacatacttt gtaaccaaga ttgtaaagct tcatttaacc
    20581 atgatgctgg cgtattaact acaaagaaca ttatagctcc actcgtaaat gcaatcaaaa
    20641 gtggacatat caatacagca tttactcctt ttaatacatc tggtaaattt tctgttatct
    20701 tcattactcc taaagctata tatcctgttg caaaaccagc taccatacca cctataaaac
    20761 ctgaacctcc aataactgcc ataaaaccag ctatcatagc aggagcaaat ccttgtcttc
    20821 ctgctattga tttagctata aaaccagcaa gtatcggtat aaataactta aatgctgaat
    20881 cgcctccaag ttgtccaaag aatccagcta tagggttgta tgtttcatga gttgggtctg
    20941 atgcaaatat accaaacata aaagctatgg ctataccaat tccacctatc actacaaatg
    21001 gaatcatttc agatacacca ctcattaagt gcttataaac accctttttc tctgaccctt
    21061 gaagcatttc ttttgaatct tctaaagtct ttccacctgg tttaatttct ttagccattc
    21121 cattttcagc gtttataatt aaatttttag ctaatgaaac attcttagca tctactactt
    21181 tcttttgtag cactttcttt cctataaatc tactcagttg aacttcttta tcacaagcta
    21241 ttattacgta ttcagctctt tttatatctt ctttagtcaa cttattttct acacctatag
    21301 ctccatgagt ttctatttta acttcatatc caagttcttg acctgcttcc tctaatgctt
    21361 tttttgccat aaaagtatga gctatacctg ttggacatcc tgttatccca actataagac
    21421 ctttattttg attattacta aaatctcctg attctgcagc tttattatta aatatactta
    21481 atatttcgta tggagtttct gcacttagta gatgcttaag aaaattttct tccattagat
    21541 atgtagctag tttagatata atcttaagat gttcatctga acctgattct ggaactgcta
    21601 tcataaaaaa tgacctaagt ttttgaccat cattatctgt aatctctcta tctgttctta
    21661 aatacatcat tacaggttga tttactacat ttgacttagc atgaggtata gctattaaat
    21721 tacccatatt agttgatgat tctttttcgc gtttattaat atcagctata aacttttctt
    21781 tattatttat ataatttcca tggtacaaat gatttgccat ataattaatt acatcttttt
    21841 ttgtttgtaa ttgtttatct agtacaatac aatcgcttcg tagtatttta cctatcataa
    21901 attctcccct ccttttaatt tgttacctca taattaattt ttatttttat aaaagcactt
    21961 taacttatct atagcattga atagattcat tttaaactct ataacttctc ttgcagattt
    22021 tatacatggt ggatatatag tatctggctc atataagctt gggttttgtt ctaatagttc
    22081 tctgcacttc ttataatatg ccgacttaac atcactcgaa atatttattt tacaaactcc
    22141 tagtttaact gcttgtgcta tttcttcatc tggatttgat gaaccaccat gaagaactaa
    22201 aggtatatct actacttctc ttatttcttt taataaatct agtttaagct ctggtttaaa
    22261 accttttgga tatataccat gtgctgtacc aattgcaata gctagtgttt ctactcctgt
    22321 tttttcaaca aaatccttag ctaattttga atctgtatat attatttcat cagtaccacc
    22381 ttcacttgat gttcctgttg ttcctattgt tccaagttct ccttctacgg acacatttaa
    22441 tccttttgct atttctacta tttccctaga tatagctaca ttctcttcat atggtaaatg
    22501 tgagccatca atcattactg atgtaaatcc acatcttatt gcccttaata tttgttcttt
    22561 attttcccca tgatctaagt gtattaccat aggtacttta ctcttatttg cttcttctat
    22621 gcaactagct acaaagctat ctcctagaaa acttagctca tttgggtgta ttgcaagtat
    22681 tactggtgca tttttttcgt tagcactctg tactactgct ttaagtattt gcccacttcc
    22741 tatattaaat gctggtacag caaattgatt ttcttgtgcc acttttagca tttccttcat
    22801 atttattaac atattgtacc tcccgatatt ttaaatttta ggttgaattt gcttcttgtt
    22861 aattttgttg ttgtttatat ttaaataata tcagggtaat gaagatcaga aaataacaat
    22921 cgttttcctt agtgaaaagg aacttttatt atacactcta agttcctttt cactaaataa
    22981 tttttagaag tctgcatcat agttaattat atttctcata tacatcttct ctttttaagt
    23041 gttttatgct ttagccttct taactctttc caataaacaa ttagatagtt tagctataaa
    23101 tttaaagata ttcgtatcgt agttagcact atattggaat caaaaaaatt aaaaagatac
    23161 tagatagcag ataccaatta ttaatacacc ataagtcata gatacttcta tcttaaattc
    23221 ttgcatatta gttacaatag aaattaaata ggactattat aaaaatcgta aggtaagact
    23281 gacatttatt ctccaactat acaaatgaat actaatatag ctccttctaa ttaaatcata
    23341 ctatataaat catatatatc ccaattttta accttacctc tttatattta ctttttaata
    23401 aaaaaagata ttgactaaat agtcaatatc ttttagtgga tttaaatacc cacgatttta
    23461 atctaatttt taatggtttc cttaacataa tcttataaat attaaaattt atctacctat
    23521 aaaatttctc cattaaattt tattacattt ttataccaat caaaagattt ctttttatat
    23581 cttttaagag tcccacttcc atcattattt ttatctacat atataaatcc atatcttttt
    23641 ttcatctctc ctgttgtaaa tgatactgga tctatacatc cccaaacagt ataccctata
    23701 acatcaacac catcaatttc aatggcattt ttcatttccc ttatatgact agccaaatac
    23761 tctattctat aatcatcatc tacagtatta tctgatttta atacatcttc tgctccaaat
    23821 ccattttcaa caatgaatat aggaagttca tacttatcat atagttgatt aaggtaaaac
    23881 cttagaccta cagggtctat attccatccc caatcagaaa tttttaagta tggattttca
    23941 actacatcag cctttcctgc cgagtcatta cctgttgttt tcatagtttt atcagctgtt
    24001 actacagtag ttaaataata actacatcca ataaaatcaa ctattccttc ttttaggatt
    24061 tttttatctt cctctgtaat atctaaatta taattttttc tttcccataa ttttagtgta
    24121 tgtcttggat aatgacctct tacatgtaca tcagtaaaca taagttgttc tctattagat
    24181 tctacaaatt taatcatatc ctctggattg caagaataag gataacttgg catagctgcc
    24241 accatacatc caattttaaa ctcagggttt atttcatgac ctatcttcac aacctttgca
    24301 cttgctacaa attcataatg aactgcttga tacataactt cttctttgtc ttcctgctct
    24361 tcatagatta ttcccgagtt agtaaatgca taaataggat ttgtagttat actttgatta
    24421 tttatttcat tgaaagtcat ccagtactta actttatcct tgtatctatt cattaccact
    24481 tcacagaagt ttacaaagaa atctattaat tttctatttc tccatccacc atagtttttt
    24541 actaaatgat aaggcatttc aaaatgactt aaagttatta ctggctctat attgtatttt
    24601 aataattcat caaataaatc atcataaaat ttcaatccat cttcatttgg agttgattca
    24661 tcgccctttg gaaaaattct agtccaagca atacttgtcc taaaacactt atatcccatt
    24721 tcagcaaata atctaatatc ttctttataa ttttcataaa aattaatggc ttcatgatta
    24781 ggataattta aaccctcaat tactccatct gttatctttc ttgaaatagt atgagtacct
    24841 gcagtcatca catctgcaat acttattcct tttccacctt tattatatcc accttcaact
    24901 tgatgggcag caactgcccc tccccataaa aattcttgat tagatttcat agaaatatct
    24961 cctccagtat aattaataaa caacttttat tacaggcgtt ccaattttaa tatgtttatt
    25021 ttctttaact attacatctg ctaaatcatt tgtgttagtt agtattattg gtgtaacaat
    25081 actataccct tcaccttcta ttttatcctt atcgaaagaa acaatcctat ctcctttttt
    25141 aactttatct cctgcattca tatgagtagt ataatacttt ccatctaaca tagtagtgtt
    25201 aagacctacg tgtattaata tttctacacc tttatcagag gtaattccta ttgcatgctt
    25261 tgtaggaaaa atagttgtta ttgttccatt tacaggagac acaacttctc cctttgaagg
    25321 tattattgcc atgccattac ctatagttcc tgatgcaaac acttcatctt ctacttcaga
    25381 taactctatt tgtaagcctt ctattggaga taatatctcc tctataccat catcttcatc
    25441 aaaagcaact tcttcattac cttcttcaca ttcttcttta ctcaatccaa agaaatatga
    25501 caatactgct cctagcacta atgctgttcc tattcctaat atatatccta ggaatggtgt
    25561 ataaactggc attgagaata tattatgaaa tacatatgca ttacaagtaa caccaaatac
    25621 accacatata ccaccaccaa tacctcctgc aatagccaat actggaatca ttcttctgta
    25681 tcttaatata attccataaa ttataggttc tgttactcct gctaaaattc ctgtaactgc
    25741 tgttgaacct gctaatacct ttaattctga atttttttta gattttctaa ccttcataaa
    25801 taaacctatt gcaataccta tttgagcaaa tactgctgca acagctgcac cttctattgg
    25861 gtctccaccc attgtagcca agttttgcaa agtaattggt gtgaagcccc agtgtagtcc
    25921 taatactgct aagaatggca tagctatacc tattgcagct cctgcaatta ctctgttaaa
    25981 ttcaaataat cccataaaaa tattagatat tatatcacca acattagttg ctattggtcc
    26041 aaataaaatc acagttaaag gaaccatgac taataatgat atcatcggta ctaagaataa
    26101 ttgtaaatcc tttaatataa tcttctttaa aagcttatca acatagtaat aaataaatat
    26161 agcaacaaat atagggaata caccaaaaga ataatcaatt gctgttactg gcatcccaaa
    26221 aaagttagca ccaccttttg ctccaattaa agctgtaaaa ttaggatcta acaaagctgc
    26281 tcctatcact ccaccaacat atggatttgc actaagtttt atagctaagg taatacctaa
    26341 gaatattggt aaaaagtaaa atacagcatt tccagctgct gataatatag cataagtgct
    26401 acttgtatca gacatccatt tcatagtagt tagtattgta agcagtgcct taatcattcc
    26461 tgctccagct agtgcaggta ttaatggaga aaatgcacct gaaattgatt caaatatttt
    26521 agaaactatt gaaacttttt ctccctgttc ttcagtattt tgtccttgta aaactgcttt
    26581 ttttataata gtttcaaagt aatcagttac tttatttcca attaccactt ggtattgtcc
    26641 tccactattt actacagata gtacatcctt catttttaat aactcttctt tgttagtttt
    26701 tgaagtatct ttcagcttaa aacgtagcct agttgcacaa tgaactaaac taataatatt
    26761 ttcttttcct ccgacatatt ttataatttc ttcagactct ctttcaaatt tcatacaatc
    26821 tatcctccat tttcataatt ttatgtataa aaatagacct aaatcaatac aattagatat
    26881 taaaaactaa ttataccaac ttaggtcatg cctgccaaac agtaacaatc ctacactatt
    26941 tactttatat ttgataatct atttaaatgc aatgttaagt acaacaattc attttcgcta
    27001 attttccatt tatacgtctt ttcaagaaag ttttttactt tttttgcaca attgtaatca
    27061 cttttatatt tcatttttat tatatctaac aatgatgtgt ctacatttgt attctcacca
    27121 cttaattgcc tttttataaa acatcttaaa tgacttataa accttgtgta gtcaaaggaa
    27181 tattcattta tttcgattcc atagtgatac tttatgatat ctaatacatt atcaattatc
    27241 ttagttaaca ataaagtttc ttgcatatct tttgtttcta actgagcatt tgcaaagtgt
    27301 atcgtaataa atgctatttc agaatcagga atatttttat ctaatttttt tcttaaatat
    27361 tctacagctt tcttactaaa attatattca ttaggatata tttgtcttat ttcccattga
    27421 agcgggcttt gaaatactaa tccttcatcc attcttttta acatataact tatatgttct
    27481 gataatgtga ttaatatact atcattaaga ttatcaccca attcttctct tcccatttga
    27541 ataatttcac ttacaagctc aacatctttt aaaggtatcc tatctagtag tgaatgtaat
    27601 tcacttaatg attcctcatt attgaatata aatttctttt caattttatc tatttcaact
    27661 aaatctttag taaatttatt aaatccaatt ccttttccca ttaatattac ttcttgtcct
    27721 gaattatctc tggcaaatac aacattatta ttaaatactt tctttataac cataaactac
    27781 ctccactgta aacaaaaaga gacctaaaaa agactaataa tactttaagc atttactaga
    27841 atttttaggt cacgcctgat tacatctatc tcagtaacga tccgtttatt tatctgtatg
    27901 ataactttat tttcattata aaataataac tgtacttagt caatatataa tttcatgtat
    27961 atctctacta gattattgtc ataagtattc actcattcta ctttttttat atgatttata
    28021 taatctaaac atgctaaaat aagttttgaa gttatttcat aacaaaatta aggtcatata
    28081 ttttttatta tatatactcg attatttatc catatataag gaaatccttt attttgtaac
    28141 tacaataact gcctaagttt tgattttctt ttatctttta cttctcctta ttaaaaaata
    28201 attcttttac tgttgtatct aaggctctaa acagtttaat catcaaatca ttgtttggat
    28261 tattaacctt acctaactta ctatatagct ttttaactgt atatctttaa tagacctttt
    28321 ggtatgctaa tccattttat aacactaatt cttgaatcaa gcatatatta cccttacaaa
    28381 gcatatctta taccctctgt agagttatga tagttgatga acctgaagaa attaccctta
    28441 caagacatat ctgatacaac ctcacttatt agtttatatg tttcagaagt atataaataa
    28501 cacatactta cttccaaatt cttaactgtt ttcttagaaa attctaatat gatttcctta
    28561 catagcgatt ataacagtgt cttattagat ttcataaaat tatacatcct ctttacattt
    28621 aaactttaaa tttttatcta ttgtttatta attaactgta tattcactat tttttatcat
    28681 ttataatact cttcttatta tcttaaaaca attataatct aaccttaatc acatattata
    28741 taaatatttt taataaactt aataattaaa taactatatt tcattatcca tcttataaat
    28801 gtttataaac tttccactat tcttcttcaa taaattcaaa caaattttct actgtagtat
    28861 cgaatacttt agcgatatcc atagctaact tcaaagaagg attatatttc ccattttcta
    28921 agtgtacaat agtctcacgt cttaccccaa ccaatttagc aagttcattt tgcgtcataa
    28981 ggagtttttc tctgtattct ttaatttttg ttttcatcaa tattgccact tagttacata
    29041 tccccctttt atcaaagtat atatacaaca ctaatttaga taaagactgt ataaataaaa
    29101 ctgcaatgag gattatacct gtatctaggt ttgatatatt tatattactt gtataaggtg
    29161 ttgcaaagaa aatagatatt attactatag aatatatcat tatttttatt gcaatcttat
    29221 taacctttga tagtataaat tcagatgatt catcaactaa atcattatat tttttattat
    29281 aaattgaata acatccaaaa actattaacc aactcaaccc tggaagagaa gcagtgcttg
    29341 gacctttaaa aatatattta gtagctaata cagctataaa aaaataagtt attacaagta
    29401 attccaagat gagctttgat cttacaccag tttctgattt agacatataa aatcactcct
    29461 taaaagttat atattctcta tattttttaa tttttgtttt cataaatatt attacttagt
    29521 tacatattcc ctttttatca aaatatatat acaaaattaa ttttgacaaa gattgtatga
    29581 ataaaactgc taagagcatt atacctatat ccaaatttga tatgtccaaa gctcttgtat
    29641 gattaactga aaagagaaca gctattacaa acatagagta taacattatt tttgatgcaa
    29701 tctgatcaac ctttaacaac ataagttttg atgcctcatc aactaaatca ttatattttt
    29761 tattatacat gagattgcac acccaaaaag ctaataaaat tataaaagta aaccaaatac
    29821 cttctccttt aaaaataaac ttttgtaata acaagtctat aaaaaaatag gatagcatca
    29881 ttaactctga aacaagattt gccttaacac caagctctga tttagacata taaaatcacc
    29941 ccttaaaagt tatatattta taacacctta tgttataaat atataacttt tccaaataaa
    30001 ttgcaataga atattgaaaa tattcaataa tatcttcata aatatatttt ataaaagatt
    30061 ttataataaa cattacaata cagtactaaa ttagtttatt tcatctatca tctcaatcat
    30121 atcatttact aactattatt ctaatgagca tataacattg aaatttataa ttatacaacc
    30181 taaatgcctc ccaatgtacc ctcaaaaaca tcatttcctg taactgaagt ataattggta
    30241 tattttatca ctgaaataaa aaaacactag tcaataaaga ctagtatttt caagtatttt
    30301 atcattcttt ttttaattat tggagacgaa tcatcagaat aatgcccata atttatctat
    30361 atgcaaattc taaatttaaa ataaagtttc agatgaattt actattctat gctatgatac
    30421 aacagaaaaa actgaaatct atgagtggta gcttcatata atcctagttc ctacagtaat
    30481 caaaactgag gtttattcta tatgtaacta atgataaatt ttattatagg agttattagc
    30541 tctatttgtg ttactataag tagtaattat atatatgata attttttaac acttttctgt
    30601 tggctatata gaaagtggtt tagaatttaa atttaaataa aatttaaatt caccaaaaag
    30661 aaatctatat tagaaaccac tactctattc caccagattg taattttttg ttctttatat
    30721 cacaatgcta taccatatta aattgaaaaa taaattttaa atttatttgt tacaattcat
    30781 taattcaaaa aaatattttg agtcatgagt tttgtaaaga tttagccatg aatttaatgc
    30841 ttctgattca aaaacatcta aaagtttcaa tacttgaaac gtaaattcca acggagcgat
    30901 tcctgaagcc gtaactaaat tttcatcact aactacagat tccgttttat aatatttttc
    30961 tccagagtaa tttggtatta ccattttaag aaactctaag tcattacttg tatgatttct
    31021 aacatctaat agcccttttt tagctaggcc taatgtagct ccacatatgg ctgcaactat
    31081 agaatcttgt attaaagctt ctttaacttt ttctaatatt atctcatgat tcatggataa
    31141 ccaagtattt ccccctggca aaattaataa atccttactt tccatattaa actcatcaac
    31201 tgtaatttca ggtaaaactt ttaacccacc cattgttata ataggatttt tatcaattcc
    31261 tacagtaatt acttttagtg gctttaatcc ttttttaaaa tatcttcctg tatttaattc
    31321 agcagttaaa tatgccactt cccaatctga catagtatca aatacataca tatatacttt
    31381 tctatttttc atctttttta ctccttatgt aattaattta tatatctaat tataaaataa
    31441 cttaactgac atattcagtc agttaagcta ttaaatttga taataatcaa ttaattctag
    31501 taaatcttca attattttct tcctaaggct tagtggttca attacttgta ttgtctttct
    31561 ataaggtaaa agtatatttg gtatgtactt gcatattact tcctcttcga gcataaatat
    31621 tattttatta ggtgttcttt ctttcaagta gtgccctaaa aaccaatgtt ggcttaaact
    31681 atctaaagaa tctatactcc cttctacaac taattgaatt atattttgtt catcttttat
    31741 ttcaggtata attcctttta taaaaaattc acttgctgaa aattctgtcg gtctgttaaa
    31801 tgtatttgat gtttctatga tttcactaat cctatccact ctgaaactac gtacctcatt
    31861 tctcatatta caaaatgcaa ctacgtacca attattattc cagtgaacaa ttccatatgg
    31921 atttataatt ctatattttg attttgcttt acgacctgca tgatagttga tttctacaga
    31981 tagctccttt aatatactat tctccaattt tttaagtact gattctatag acactttccc
    32041 aattggtttt attacttcaa gggctcttaa gtggtttgtt aaaatatctt cttggttttg
    32101 atttgaatac atttttaact ttaatatcga tttatctaat gcttcgtcta agaagtatcc
    32161 ttctttttta gcaaatagag ctgcatgaag tagagatttt tgctcttcta tatcaaaaaa
    32221 aagaggtgct ttagtaaaat tatttaaaag gctataccca ccattatgac ctgtatctga
    32281 aattataggt actccactcg cagaaagtac atcaatatat cggtacactg ttcttatatt
    32341 tatttctaat ttttcagcta tctgttttgc tgtaattttt ttgtcagagt ttaacatcca
    32401 cagtatagac atcatattgt caattttagc catgtagttt cacctctttc tatttgttat
    32461 ataaagttat tatataactt tttgattgat tttttacaaa aaaacattag taaactgcta
    32521 ataatttata ctaagattta tttcaaatat ttatagatag tctttttcta tagttaagtt
    32581 atccattaaa ttacatagta gtatatccat tttagttgat atattttcag ctacctccct
    32641 tgaatattat tacaataagt ctaaatcttc tttcttccat aaatatatta tgaggaaaga
    32701 ctatcatctt taatggttta atagtataaa agatattaat ttaaaaaaat ctatttttat
    32761 atcagatggg tattgttttt tataaaactg aaaaaatcac ctaatagaat ctatgatttt
    32821 ttaacataac ctccataaat tagtaccaat tatcacttca aattcaaatt tttatctgaa
    32881 gaatatacaa tcatatttcc atactcaaca taatattgag tgtttggctt gtatttacaa
    32941 attattattc taacaaaaac aactatacat agattaaaat caccttctaa acataattat
    33001 taaaatttaa ttaatgatat caattccatt aatctctttt aataaaaaat tctcttttct
    33061 atatgaaaat tatttcaaaa cttttaagtt aaatacaaaa atgactataa atagattttt
    33121 atttacacaa atctatttat agtcactata gtttatccaa taaaataata aaataaatat
    33181 gtacacaata aatgttattt acgtcttaag tatttatttg ttagattttt aattaaaaaa
    33241 aacaagctaa tgaacattaa tgctaaatct agcattatat tactttgatc tcctgtttgt
    33301 acattttgac ctacattact atctaaactt tcatctgtat ctatatttat atctggtttt
    33361 ccattcccat ctgtatctat atttatatcc ggctttccat ccccatctgt atctatattt
    33421 atatccggtt ttccatctcc atctatatct atatttatat ctggttttcc atctccatct
    33481 atatctatat ttatatctgg ttttccatct ccatctatat ctatatttat atctggtttt
    33541 ccatctccat ctgtatctat atttatatct ggttttccat ctccatctat atctatattt
    33601 atattcggtt ttccatcccc atctgtatct atatctggtt tgctatttcc tccattatct
    33661 gggtctggtt tactatttcc accgttatct ggatccggtt tgctatttcc tccattatct
    33721 ggatctggtt taccattgcc accgttatct ggatctggtt tgctatttcc tccgttatct
    33781 ggatccggtt tgctatttcc tccattacct gaatccggtt tgctatttcc tccattacct
    33841 gaatctggtt tgctatttcc tccattacct gaatccggtt tgctatttcc tccattacct
    33901 gaatctggtt tgctatttcc tccattatct ggatccggct tgctatttcc tccattatct
    33961 ggatctggct tatttagatt tggattacct gtatcataac ctcctgagcc agtatctaca
    34021 tttttatcag gcttccaata atcatctact ccatcattat ctgtatctat tgctggacga
    34081 ttataatctg tatcagggtt tccatctcca ttggtatcta tatttttatc aggtttccaa
    34141 ataccatctc catttccacc ttctgtacta ggcttccatt cacctgtatt atcagtatct
    34201 atgttaatat ctggcttcca atcaccactc gtatctatat tgatatctgg ccttccatca
    34261 ccatcagtgt ctatatttat atccggtatt ccatccccat ctacatctac atttgtcata
    34321 tctaacttta acttcttttc atcctcacta ccttcaatgt aatttatatc atctccaata
    34381 tattttacag taatagtttg ttcagtagta aaaatatgat gattgctatc taaaggtctc
    34441 cattcaatag atgcagtact ataattatga ctctcactac ccttattatg agtttttaat
    34501 tctactggat ctttatattc ctttccatta atatagaatt gtacgtaacc tcttggagag
    34561 gcacaatctg gtccttcttt cttatctggt gaaatagtaa catttaatgt aaacgtatct
    34621 gttggtttaa aaacatcagt tttagcttta gactcttcta caactaatga agtttctgta
    34681 tcagcaggaa taatttctgc cttataatat gctctatgcc cccaataagc ttcattaaaa
    34741 ggatatacat ttgaaaactg tgttgatgta atgcttaatt gatattttcc agcatcaact
    34801 tgctttgtaa ggtcatcact tcctaaattt gtatctggtt ccattgtttc ctcatttaaa
    34861 cgttgagaac taatagtcat aaatttttta tctgtgagtt tttcaccttt aggagtaatt
    34921 attggaatac ccccatcttt taatatccaa tctaaaaata atttttctac atcttcttca
    34981 aatggtaatc catcatatct tttaataatc aaatatggtg gagtatcatc aatagtaaat
    35041 tcttcatatt gctttaaaaa ttgagaacct gaacctatat ctttaacata aaaatcatct
    35101 ggttttatat ttcctccagt aattttatct ataacaacta aattaacaga tacttcagat
    35161 cccttaaatt gatcagaaaa ccataagtaa agaacccctt gatcatctgt ataactaggt
    35221 gctccataag gataaatttt tccatcaatt agcattttaa aatcatcaac taatgtagta
    35281 ctaactttat cttgtcctaa atactttaaa gatattttat tcataaaaac ttttgttttc
    35341 ttttctaaat caccgtatgc aactgctcca cctaaacttt tgaatgttgt tgcttttaat
    35401 gaaccacctg ttactataac atctcctgtt gtagcactta ggtcaaaaat ccaatctccc
    35461 actcctgtca tataaggtaa aatcgttcct ccagttacaa gtatacgata tcctctattt
    35521 gttcctggat taacacatcc atccccaaat gcactaccat gtgcacctcc atatgaatag
    35581 gaaagtccac cattaattgt aatatttcca ctataaccag aacctgtgct aggatctact
    35641 actgagggac ttaatgcatt ctttgttgtt gtatagtatc ctccacctat tccagctcca
    35701 tggtatgcac ctttagcagt tatagtacca ccattgattg tgatatctcc accactacca
    35761 ttaccgcctc cacctattcc agctccatat cctgtattac gactcgattc ttgacctata
    35821 gcagttatag caccaccatt aataataata tttcctccac tttcagtatt tccaccgcca
    35881 atacctgcag aataataatt tccaacaact tttaaacttc ctctattatt actatctagt
    35941 agtgtaaggc gatctccttt ttttaacttg tctccatttt ctagagttac atctctagga
    36001 atttttccac cctcaggaac tataaactct ccttcaatat ctctatttac aacagaatca
    36061 tcaatagtta gagtagtacc ttttcctgct ctcatacctg gataatatac atcacctgac
    36121 tctagcttgt tttcactacc atcttgtaat ataacatgtg catatgcacc accatcttta
    36181 tctggtgtta aagtaaatgg gctattagat gcagttttaa tatttactcc atcaaatatt
    36241 aattcagctt ttacatcttt aggtataaca atcctatgtg ttgttggagt atttggatta
    36301 ttattttgaa ttgtcatttg ttttccacta agaatagtta aaacgccact tgcaaattta
    36361 aaatcgacat ttatttctcc accactaatt ttaaagtctc ctacagatgt aggatcttct
    36421 gcagtggctt cttccacaac taatgcattc atatcttctt ctgcatttcc tgcattagaa
    36481 tcttcttctg catttcctgc attagcatct tcttctgcat ttcctgcatt agcatctcct
    36541 tctgcatttc ctgcattaga atcttcttct gcatttcctg cattagcatc ccctcctgca
    36601 tttcctgcat tagcatcccc tcctgcattt cctgcattag catcccctcc tgcatttcct
    36661 gcattagtat ctccttctgc atttcctgca ttagtatctc cttctgcatt tcctgcatta
    36721 gtatctcctt ctgcatttcc tgcattagta ctttcttcta tagtatctgc gaaagtattt
    36781 gacgcattaa atgatgctac tgatccaagt cccattgaga tacttaaaat cattgatagt
    36841 gctttctttt tacatttttg ttgcataaat tccccctccc cttaataatt tttaatatac
    36901 actgtataca tccttatata cactctaata attcttaata tacactgtat acatccttat
    36961 atgcactcta ataattttta atatatactt aaaacttata tttgtcaatg aatatactgt
    37021 ttttttcaac cttatatatt ataagtattc attatttgct aaataactac aataaaaatt
    37081 ttaattatat aaaatttatt taatttttta tataataata ctaaggggta attaattgaa
    37141 aaactagtaa aaatattaat aaatattatt tgcattattg cgctaacttt ctctagcctt
    37201 tcgatttaca taaaattatc tgaatataaa aaagctgatg aagtatatac ggaactcaga
    37261 gaaaacacta tcaataatag caaacatcaa gaactttatg ataaaaataa tgattataga
    37321 ttctggctta aaataaacaa cgtaaatata aactatcctg tagtacaagg atataacaat
    37381 gatttctacc taactcatga tttttataaa aattatcttc ctttaggtag tattttcatg
    37441 gattatagaa ataattttga aaatgataag agtttaatag tctatggtca ttatatgaaa
    37501 aataaaacta tgtttggtca actagaaaac tatactgatg aagtattttt caaagaaaat
    37561 aacttagtag aaataaatta taaagtacaa acttatactt atgaaatttt ttcagtatat
    37621 actgctgact taattaacag agattattta agtatacact ttaataataa tgatgagttt
    37681 aaatattctt tgaattatat aacctaaaaa tcagtacaca aatctgatat aaaggtagat
    37741 atttcagata aaattataac tttgtatact tgtaactatg aatttaaaga tgctcttact
    37801 atagttcatg ctaaattaat ttcaaaaaag agacctataa gtaggtttgt gtaaatgcag
    37861 acttatctac agtctttttt gaacgtaaaa ttggtaagta tggaataata tttatattaa
    37921 acttttgtag gcaaagaaat gaaaaatcaa agagaaataa ttattacaga tttagattac
    37981 caagctgaat taagaaagtg taaaactatg gaggatgtag ttggtgaaga tagtatataa
    38041 tagcttaact tataaattat cttggtgata ttaatgaagt ttcaaagtat atattattta
    38101 ttgatataaa taaaattaaa tctgtttcta atagatgtac ctttgtttgg agacaaacta
    38161 ttgaagaaat ataactaaaa tgttgaagta gctacctgac tttattttga aatgcgaaca
    38221 agattttggc attaagataa tttagaaaga atcactggta cacaaggatg tgaacttaga
    38281 atgaatagaa gtattcaggc ataaggagct ttcacccaaa taaaacatag tatgaacttt
    38341 aaaagaattc taatcaaagg caatcaaaat attctttcag agtttatacc tgtagtgtta
    38401 gcctatagta tttctaagtt tcacaaataa gataatcatt aataaatgta gtatgtatat
    38461 tcatcctctc aaaaatattt tttaatctta cactttgtaa attattctat tttaaaattg
    38521 tatttttata cctttatttc ttatgaccgt tttcaagctc aaattttgca taaatataaa
    38581 ttaatgaaaa gcaagtaaaa aaagaactgt cgcacagcaa tttaaaatta ctcattcgac
    38641 agtccttatt tcgtggatta atgttggata agaatcacaa gaataatacc cactatttta
    38701 tttgaactat ccatctagtg cttcaaacac atctctaaga tactcatttg gttcatcatt
    38761 aaataaattt aatatctttt ctgctactac tcgacctaat aaaggtggta ctgcatttcc
    38821 tatctgttga tatacagaat cttttgtacc taaaaactta taactatcag ggaatgactg
    38881 caatctagcc gcctctcttg cgctaacagc tctgttttta ataggatgta tccacataga
    38941 ttttcttaca tttacaacag tatctgatgg cttatcatat actagtcttt tataaatagt
    39001 attttgagtt ctttctggat cagcataagt tccttttaac tcatctggca aagaatgaaa
    39061 attatttcct tgatttatat attcaaatct ttttagtgcg atatttcttg tatctgtaca
    39121 tacatgattg tatattttat tgctatccaa tattatcttt ctataaaaat ctttttcaat
    39181 attattacta tttttttcaa aacaataatc catactacct ttagaagcct catattcagc
    39241 taaatcgcct attgcatctc ttactgtaac ataattttct ccaactatag gcttaggagt
    39301 ttctatcttt ttatttttta agaagtcttt ttttgctcct atcaatacaa atctttctct
    39361 attttgaggt actccataat caattgaatt tagtgcactt ccatttaata catatcctag
    39421 atactcaaag gatgcattta tataatctat tataatgtaa gtatccattt tagctataat
    39481 tttattatca tcaagtgtat tttcgaaaat tacagattca gattttaact catatattcc
    39541 taaaaataaa ttttgaatat ccaagaataa gttcaaataa aatatttgtt catctgataa
    39601 atttctatta tataacatat tttctaatat attttttact ttatttatta attctaaata
    39661 ccattttggc atttttcccc ttgtttccaa tttttctatg gtagatttta ctttcttata
    39721 attagatatt ttatttaaat aatcacatat atcctttttt ttcttaataa tta
//
LOCUS       DAMJWS010000029        38973 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMJWS010000029 DAMJWS010000000
VERSION     DAMJWS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.029"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            69..1073
                     /locus_tag="PC399_001604"
     CDS             69..1073
                     /locus_tag="PC399_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2592468.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDMDLFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLKSCDLA
                     ICLYSQKDEKKVAILKLDYNKSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDEYHLRVLDKDAEKEEANSKFITEFLNATKVKDDKYKTKMFKSTAENWITNAL
                     GNDIKQAEDVRSILNYTLKEKHEIDINNFVDKTIKDDKLKDSFKEHMEEKGLDKGFSI
                     DKKWVEKKLKKRNIKTDNGFDIKGNLTDFEDPMKYTVRQNQNGSIDIIIKNVKFYEEK
                     "
     gene            1082..1249
                     /locus_tag="PC399_001605"
     CDS             1082..1249
                     /locus_tag="PC399_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592469.1"
                     /translation="MTNKEMCKSKNLDEREIYKEFGKEICASCISDRVDCESKDCDIA
                     YENWLEKDAER"
     gene            1334..1606
                     /locus_tag="PC399_001606"
     CDS             1334..1606
                     /locus_tag="PC399_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592470.1"
                     /translation="MITTQMYKINNEIFLNDLCLEENKEMTIWVEKNKDTHEFIWLKI
                     ANVNGKLAIFIQDIENAVIKEWQGQIAYKKVIYQIEYEQVEKGEID"
     gene            1629..1859
                     /locus_tag="PC399_001607"
     CDS             1629..1859
                     /locus_tag="PC399_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDF2592471.1"
                     /translation="MIHELKILPQYFKEVVNGNKNFEVRKNDRGFKKGDLLVLQEFDG
                     EKYTGLEIRKEIIYLLDNSNYLQDGYVVLGIK"
     gene            2013..2411
                     /locus_tag="PC399_001608"
     CDS             2013..2411
                     /locus_tag="PC399_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592472.1"
                     /translation="MQKDVWLYSWDNKYLSSDEYESKEEAIQAAKEELKEFGNFGESI
                     YVGKKEEVSIPNIDIEEALECIQKKIDDEVGECGEDWFENICVEDMIILSNRVNEVFE
                     KWIDEFGYKPYWFKLTDKEEIELNEVANES"
     gene            2401..2844
                     /locus_tag="PC399_001609"
     CDS             2401..2844
                     /locus_tag="PC399_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2592473.1"
                     /translation="MKVNFTIDGEPKAKARPRMSTANGKAYTPDQTILYENWIRLMYN
                     STVKHFFEGNVKMTVICYFDITKKDREALQKNKVNTKAYKNAIDKVEGLIRPNKKPDL
                     DNIIKSVADSLNGIAYKDDSQIVEVVSKKYYSDRPRVEVELEDVS"
     gene            2867..3043
                     /locus_tag="PC399_001610"
     CDS             2867..3043
                     /locus_tag="PC399_001610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592474.1"
                     /translation="MKKFIYINRHRVYIRNLFKISAIYKENHYIREFKSECLSNNTLN
                     AVIESSVRIGFGSK"
     gene            3148..3654
                     /locus_tag="PC399_001611"
     CDS             3148..3654
                     /locus_tag="PC399_001611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592475.1"
                     /translation="MSKTKKEFFNATKKQLSNYKQLSTNIIKLKNEIQMLKDNSVGDL
                     MKGISYDSVKTGKTNKTSNMIEDAIVNVSDLITEKEIELYEAEIIKSTIDLAIRNLKP
                     IHRQIIELKYIDGLMWQEMVDIVHLEERQLSVRASQAISSISIALFGKKALIEQEPLF
                     ELLDYKLN"
     gene            4195..4872
                     /locus_tag="PC399_001612"
     CDS             4195..4872
                     /locus_tag="PC399_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDF2592476.1"
                     /translation="MLKILQEKNVKVMWSKNGEEVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKPEAKLFTKWVT
                     KTLKQIRIHGYYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVYYATSQGSNKPQMYY
                     KHFTELVRKKLGIPKGVKRDELNQSELFDIQALERIISMKLPKLIDKDMNYKEVYKKI
                     KELIEMI"
     gene            4935..5636
                     /locus_tag="PC399_001613"
     CDS             4935..5636
                     /locus_tag="PC399_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592477.1"
                     /translation="MQDVKEKVKQDYIKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELIQEISIKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLKKSDTGDDSKIWV
                     EAIQNIAMKRGVNNG"
     gene            5629..7038
                     /locus_tag="PC399_001614"
     CDS             5629..7038
                     /locus_tag="PC399_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HDF2592478.1"
                     /translation="MDKALLTLLDCYWDNPVWFAEDMLNFKADKWQSDVLMALAQTPK
                     VSIRSGQGVGKTGLESIATVWYLSTRPFPKVVATAPTRQQLYDVLWAEIAKWLSNSKV
                     EKLLEWTKTKVYMKGFEERWWATARTAVKPENMQGFHEDYMLFVVDEASGVADPIMEA
                     ILGTLSGAENKLLLCGNPTRTSGTFYDSHNRDRDLYKTFKVSSLDSPRTSKDNIEMLK
                     RKYHEGSDPWRVRVLGEFPKGESDSLISLEAVETSTIREVNISNDYILNIGADIARYG
                     DDETIIAPRIGGKVFDLLTYSKKDTMETVGNILRAVDKFKNMYHQINRVKIKTDDDGL
                     GAGVTDRLKEVIRHERLKYEVIPIQNGSSAIEKDKYYNKASEMWDNMREELDANLSSF
                     IQNKEAIIQLPNDDKLIKQLSNRKYTVDSKGKIQIESKKEMKKRIGESPDRADAVIYS
                     FAENNNTDLSLLKGGSVWG"
     gene            7029..8531
                     /locus_tag="PC399_001615"
     CDS             7029..8531
                     /locus_tag="PC399_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2592479.1"
                     /translation="MGIISYVKKLFKRPAGEIMRMSSGNIGVYKLDDSRVDYELAREL
                     YQNKNANYKLGSSFVRPIVNSTTGFMGVPHFQIEDEEAQYILDEFVLDNTSKMLKTHT
                     DSLKQGDCYIWITREERENPLYPDKKVRLIYNFISPEEVKEIILDPTTKEPIAYILES
                     QNEWTDLGENKRKAKVKQIITAESRFVEVEGDKIEGLEEGETPNVWGFIPIIHFKNEA
                     DETLKYGQSDIEPIEPLLKAYHDVMLHALKGSKMHSTPKLKLKLTDVASFLAHNFGVE
                     DPVKFAKEGGKINLDGHEILFLNKDEEAEFVEVKSAIGDAKELLKLLFYCIVDVSETP
                     EFIFGVHTPSALASVKEQMPIMVNKIRRKREQFTNSWQLLARMVLIMSSNSSGMKYSS
                     YDVTIGWDEVNPRDDKELAETLEKVCSALDKALEGGFISEESTVNFLAQYIDTMSNYI
                     SDDPEIVGEREKIIKTKMLKYRLDDSQGLNDESNEIEKEINKIKDNNGNG"
     gene            8518..9531
                     /locus_tag="PC399_001616"
     CDS             8518..9531
                     /locus_tag="PC399_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592480.1"
                     /translation="MAMDKSTSELITVAGEYKKWALEARKKFIDLRLKQDDEIRTMYI
                     NITRNITKEIRKGNLSDFNKVRLKQILKQLTQEIKILNEQLVFNFDEYLNKNVETATS
                     YSKNILINAVETAQITKVTKTMIQKAFYDINIRTVEAYYTRVKDGLFLSDRIWSKCKK
                     YREDMKVILQTAVTEGQDCVKTAKMLDKYVLKGKKTLVDEYPNMIKRIGNRVPQNISY
                     EALRLARTEMTSAYGDGVLASAMINPATIGIQFMLSMAHPHTDICDEICGEDNFGLGK
                     GVYPINEAPVYPFHPHCLCIMLTVVQPLDILVGRLKNWIKNPMNDVPLEMWYQEVYGN
                     LNF"
     gene            9551..10885
                     /locus_tag="PC399_001617"
     CDS             9551..10885
                     /locus_tag="PC399_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592481.1"
                     /translation="MNVITGEMDSMNALISSIKPSDIPLAKDIDIEALKSIDDDPLEV
                     VVEIPATKSKRGWNYTAKSLKDIVDYTNENTLNGFLGHQKAENISTEFALPVTHWIGA
                     EMKGDKAYFRGLIDADATNLKRWIRTKRIKEVSIFGYPKLKKSAKGEMNVIGYEPLSI
                     DWTPLHRPGMPTSIVGMEMSPNGEQLDGTFEALRIDLREALKAKFSINDNNSYLYIQN
                     IRYDNNTVIYELEQNGLCKLYSIPFTIVENKINLGEEIEVIKKISYEAKGEMKGEENK
                     LEGKELIKNVKGLLQTGEISYSEVIQGIGLTKEIVTGEMEDVKSSLKAEKELREVKKV
                     LGIVGEMDTVEVAKKASKALENEKKEAWNCIVNKVIKDKVSGEIAQTLVKKMLNVEEG
                     SSEEVITGEIENILNDEFVKNTMSNMYKDNPTTTGLLNSSNNGSLTTKKNRI"
     gene            10901..11284
                     /locus_tag="PC399_001618"
     CDS             10901..11284
                     /locus_tag="PC399_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDF2592482.1"
                     /translation="MAFKGQPTPSTITQITRAKISDGKSVRVILSEGESTKTQQFYLI
                     NGFFGVAMQDGEKGDEVTLQIEQAEYETDNIVTSEAFEAGKLIYWDNTAKKFTTTSAS
                     NRLVGRVTDGKDSNNVIWFILLPQQ"
     gene            11303..12340
                     /locus_tag="PC399_001619"
     CDS             11303..12340
                     /locus_tag="PC399_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDF2592483.1"
                     /translation="MAFKVISQENLLEQKRKETLQEDIPFIVNGEMEYVTKKISNGEM
                     ETLELNKPLGEMMTFSSTSNLKELLRKVVLDVELGREQVQLLYKPIYDSIADSNLPQV
                     MDAKWALQGNCVFLEHIEGEEIKFGTINAENGPVARIQTYATGFEYTKEMKDFNQTFS
                     VEILNKSIGESYNALLNHIHLSPIINFNYKASNKTAFKGETNDPIWLGIWRTLTQAQK
                     DTVIAKRQGNILMASSADQIEIEMALNGGHLLNGSMYPSIKNISTVIYYDGWEVTVGK
                     KTYSYKGVTPGKGYLIRPKRGFKELIKRDLTTEVGNADLSKLVENQIVGHCYRGAFAA
                     VEENVQEISFR"
     gene            12380..12718
                     /locus_tag="PC399_001620"
     CDS             12380..12718
                     /locus_tag="PC399_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592484.1"
                     /translation="MTPARDLIEKLRLLLNDKDKKSFTDEELNLFLEEADCIYCAASQ
                     GWILKSLQYENTVGEMYEYKVGQETYKSSSIKDLVSVAYQNADKFKDMCTNKKEKGSF
                     MLGISTEFEI"
     gene            12715..13092
                     /locus_tag="PC399_001621"
     CDS             12715..13092
                     /locus_tag="PC399_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592485.1"
                     /translation="MINIDRRRKDIIRTININPTNITITSIKKTEIDGAFEETETEIK
                     CVVRIFNEKTAEKQISSEKQGTFSSIRTYGMLVSNDVILEVNSRDSLEFECIYGRMKI
                     VNIYPQIVKGELCGYQCSLERID"
     gene            13094..13504
                     /locus_tag="PC399_001622"
     CDS             13094..13504
                     /locus_tag="PC399_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592486.1"
                     /translation="MSAFTNAINDINRKKAGMLVLCMSASAMLEGEAKANARWTDRTS
                     HARQSLNAKTLSGGNNFIIRLSHGAEYGGILEEGSKPHVITPKSAQALYWRGASHPVK
                     SVQHPGTKATPIIKPTIDKNIGKIGNMIFRYWSD"
     gene            13505..14326
                     /locus_tag="PC399_001623"
     CDS             13505..14326
                     /locus_tag="PC399_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592487.1"
                     /translation="MRAGIRKALIDNIKELKGCYEPNVPNKDTKKPYMVVVQGQDNDH
                     GETIGFERSIEVWIYEGRTTFKKLDKLTKQVVEVLDMNTIVDESENEAFTCIYKGTSE
                     NDIVVEEWDAIARGIRFSVIALEDKEDTTNDRWVEALSRHTKDLLEIESYKDNWKKNF
                     IAPCALWRTTHIENKRINYHLIEITKTMKCHVVSKNKDEIVKLLETLETSLIIDKRVR
                     LREDKNMYLTLVSVVEDRESDMFTTGQLTAVFKMIGKIKREGPTMDKIYGNGNLK"
     gene            14339..14542
                     /locus_tag="PC399_001624"
     CDS             14339..14542
                     /locus_tag="PC399_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592488.1"
                     /translation="MAETNNKKINVSKQEEKYLKSDFIKNSEALGYGKMVVAGALFNC
                     KKEELTKAEFEKLIKDFLEREVK"
     gene            14544..15965
                     /locus_tag="PC399_001625"
     CDS             14544..15965
                     /locus_tag="PC399_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2592489.1"
                     /translation="MATGTWNEKERKEIPGFYNRFKTQAEKSTNTGLKGRLAMPIRAN
                     WGDVGKVVTIKNDLRQLKNLFGDDMNYSAFKLGKLALLGNVKELLLYRLVDGNQKKGT
                     LTLKDTTENSAKDVIKLETKYPTARNFNVTIKSNLVDSDKKDFIFFENTKQLFSSSIK
                     GTIDEIVLEINSNLDNEYVIATKVADSDTILANVVNQALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVADEALQETTKAWVAKNKELGKDILLFLGGKTEDNIKQINDKS
                     KSFNDENIVNVGSSAYYENIKYTPSEVAVYIAALSVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLVLDFDDGDVIIVDDVNTFKKYVDDKNEAMGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDATGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWDAVKVDVMKKIYGTGYLG"
     gene            15981..16403
                     /locus_tag="PC399_001626"
     CDS             15981..16403
                     /locus_tag="PC399_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592490.1"
                     /translation="MGKYDENIIDAANVVDGSNARIIIDGEEEGYGTEFTAEVENDKK
                     TFRVIGCKWELNKASTQKGTFSLTVLKTTSKWIKKGFNKFEIITEIENPGLVGYERIR
                     YKNCMVDKIQLASIKSDENIEIQIDGTFEGFELLDEIA"
     gene            16480..16953
                     /locus_tag="PC399_001627"
     CDS             16480..16953
                     /locus_tag="PC399_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592491.1"
                     /translation="MENLDKEFLNEGIEEERELTKDEIAKQQEDNIIMKLTEDAILPE
                     KTIFVKRLDIPLTLRALTEKEISALQKKYTKVTKVRGRRESKLMEDEFNIALIEKATI
                     VPNFGDARLLNSMKVSNGVEFIRRKFLAGEIALISDEVLELSGFYEELSDGDIKN"
     gene            17134..18141
                     /locus_tag="PC399_001628"
                     /pseudo
     CDS             17134..18141
                     /locus_tag="PC399_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            18342..19220
                     /locus_tag="PC399_001629"
     CDS             18342..19220
                     /locus_tag="PC399_001629"
                     /inference="COORDINATES: protein motif:HMM:NF018205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDF2592492.1"
                     /translation="MKNLCKNCGKEFNGKVNQKCCSRKCSNELKFPKVITKCDYCGKK
                     IEKRPYEIERSKHHYCSSECRFKHQKLTLKGESNPNFKKANIDVKCSNCGKKINILKC
                     TIKNSDGTTKKNFYCSQICKAEHQKKILKKESNPNFKGGDINCSCDNCGKEINVKRHK
                     YKIHKYHYCSQDCKAKHQQVILVGKNNPFYKLDLSEEDRVKLRCIDGYNTWRRLVYER
                     DNYTCQCCSDDKGGNLEAHHLDGYNWCKEKRTNVNNGVTLCEICHNKFHNLYGFGNNK
                     KEQFEEFMKNNKNQAS"
     gene            19482..22766
                     /locus_tag="PC399_001630"
                     /pseudo
     CDS             19482..22766
                     /locus_tag="PC399_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            22846..23766
                     /locus_tag="PC399_001631"
     CDS             22846..23766
                     /locus_tag="PC399_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2592493.1"
                     /translation="MNFFKRKDDIKNFKLMYIKGHPHFNNELTCTMYIDKKLVSVYTL
                     QQVPISEIKLLDIEKVFIDNDMLIIESKKSVLYFKSNSKDKICNIINQNIADMQDKKT
                     DTILRNKNKIVKQGVMKMDYYNSVGVPNCRGEAISIIIDNHNACLVIESKLLKEEKMR
                     IKFSDLYSVNKYSEKEIAYNNKSVVGRAAIGTLFGPMGTIVGGLSGTGNKKSKTEKLF
                     ISIEYFSHGELREIIAEDSILNFHSQEFIDNIRRKINISKNNLENNKEKQIKSNDDAI
                     QQVKGLKELLDMGAITEEEFNKKKKELLNL"
     repeat_region   24024..24446
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=24024..24052
                     /rpt_unit_seq="attttatattaactatgtggtatgtaaat"
     gene            24739..24906
                     /locus_tag="PC399_001632"
     CDS             24739..24906
                     /locus_tag="PC399_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592494.1"
                     /translation="MPSKKIGRPTDNPKGSRITIRLDEESKNILEKYCEKEDIDKAEG
                     VRRGIKLLKDK"
     gene            25025..25843
                     /locus_tag="PC399_001633"
     CDS             25025..25843
                     /locus_tag="PC399_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HDF2592495.1"
                     /translation="MNELMNFEGKDIEIFEYNGQVLFNPYDCGRCLELSDSAIRNHLS
                     KMNDTQAILLKNSAVLDKDIRKLNNAGEKFLTESGVYKLIFKSQKEEAEKFQDWISDE
                     VLPNIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLKELLDDAGFDNKSKLLT
                     AKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPAQMAVCEIIKKIDLEEN
                     EVKGVWETNGSWTGTVNKYTKSVIDKISKWIEENNRPTKIQGEKKNFHVVYKIE"
     gene            25897..26055
                     /locus_tag="PC399_001634"
     CDS             25897..26055
                     /locus_tag="PC399_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592496.1"
                     /translation="MCENLLDSIDIEKRKEECRIKLLKIRETDIDIYNKIESIVYKLS
                     EKKLEKNK"
     gene            26665..26817
                     /locus_tag="PC399_001635"
     CDS             26665..26817
                     /locus_tag="PC399_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592497.1"
                     /translation="MDNFLIGVLASLTATLIAYVIGKLIKKAKSHSRAKSDLIVEFKF
                     MFKSKK"
     gene            27119..27790
                     /locus_tag="PC399_001636"
     CDS             27119..27790
                     /locus_tag="PC399_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2592498.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVNPQDSLSIKKEKKFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPFQYDASYCRYSELKNPIEVVAMLEKWVDQAEPLRLIIT
                     GFGYNGLVTISSFSNTQTAGREEDRDIEITFRTYRELKIETLKKETKSNTKTDLKDNR
                     PNTQTKSKIYTVKASDTLYKIAKNLLGKGSRWPEIYNIPENKKVIGKNPNIIKKGQKL
                     VIPSK"
     gene            27787..28749
                     /locus_tag="PC399_001637"
     CDS             27787..28749
                     /locus_tag="PC399_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HDF2592499.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLVEPKQLKDI
                     NIKKGDKIILIDIAYESKKEETIFDGVVWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     QFKENTATQRIEYYCKQWNIPYYNLANTGKKLAKVIHKTNILDMIKKDLKETATKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDADKFGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKSKEYYVIDVTHTLDSRPKMKLNIGSLEYIRRKFYNND"
     gene            28742..29107
                     /locus_tag="PC399_001638"
     CDS             28742..29107
                     /locus_tag="PC399_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592500.1"
                     /translation="MTDARFNGIARILKENMNKSVANGTFGMGCELAEITANGLKVNG
                     YKDEIQDYLVLENLTLKEDYFTFSDEALSGEYRHKHKIETPKELKPLRIGDNVLVAVM
                     GAEFVVIGRVVNAKPISSK"
     gene            29082..29540
                     /locus_tag="PC399_001639"
     CDS             29082..29540
                     /locus_tag="PC399_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2592501.1"
                     /translation="MPNLFPQSETFETVELKNNDENELDLKGSFLFDFEKGEFVKNAD
                     GTLKKCDKVQAYKQWCQKAILTPRYKKAAYTNIYGSEIKDLIASNLSQSAKELEITRL
                     IKETILVHPYTKEVGEFSFNWLENSRLVEYEFDVLTIDDENIVIDGNIKR"
     gene            29549..30685
                     /locus_tag="PC399_001640"
     CDS             29549..30685
                     /locus_tag="PC399_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2592502.1"
                     /translation="MERELPIPVFLTEDEDSVHERMLSNFQDVSTLEGDFIYDATRPT
                     AEQIAELKQLGLQNNLKIAFPQTSYGTYLEWLGECKGVFKNQPTKATGVITFTGVQGT
                     IITKGTIVTTIATDEKQSIEFELLETKTIGENETVDIKAESRIVGTIGNVSKGSISVL
                     LGSISGVKSITNKEDFRGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVDGV
                     GYAYVVSEWAGAGTVKVLILDKNRKAATQELIDKVQEYIYPLNISEGENRDGKAPIGA
                     LVTVVTPDTLLINVKASFIFSNGFSEETVLNNLKTKIDKYLDKIDLGGTVSYNAIQAI
                     VGSMMLTDEGIEDFSNLTINDVKENIKLQDQVVGIGEIVNEVVG"
     gene            30682..31311
                     /locus_tag="PC399_001641"
     CDS             30682..31311
                     /locus_tag="PC399_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2592503.1"
                     /translation="MIASKKGKEMLLTLSPIYEQSIIMQSLYEAIGSEFDNLELLDEE
                     IELQLFPQSATWGLGFWENRVGLITNLDEDMETRRRKVIAKLQSKYIMTPKRMSMILQ
                     SYTGANIKINENISPYTFGVELTSTQGFPKDLEDLYKRVNVIKPSHLAVSYKLVSLLK
                     SKTYFAQTAIMSEEITIYPYTSKEVKASVKAKFALAHNMSSETLTVYPR"
     gene            31324..32430
                     /locus_tag="PC399_001642"
     CDS             31324..32430
                     /locus_tag="PC399_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2592504.1"
                     /translation="MADEQFYTILTNIGKAKIANAGMLGKPVVLEKIQAGDGGGNYYN
                     PTEEQTALKNKVWEGNINAFDNDENNPNWIIATACIPGSIGGFTVREMGLIDNEGDMI
                     AICKSPETYKPKIDNGAMKDLYLKFIIEVSNVEKVTLVVDPTAIFLTKKDEEKILTNI
                     NKLDTKIDTTKIELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTITI
                     DDWVEDAESGFKATVTHGLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSEVKVE
                     LNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDSISRIQLIDTSISITDANNRF
                     TSNKLDGVLEEIMVEISGQRTKGITIVNNLIDMI"
     gene            32444..33082
                     /locus_tag="PC399_001643"
     CDS             32444..33082
                     /locus_tag="PC399_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592505.1"
                     /translation="MTTKLTDNASLEELMTTLENVQADFQTGKNNISSALGSPFIGTD
                     KFGTTKTKIETLKNVLVETINSKNVSATSSETFTNLIEKVNWVFQSIEIFSLKNRIQA
                     TIVNTPSIVYNEVSSIKGTLRFTGELQSSKMRADYATAKIEILCGNRKEYFYVTDNTP
                     GASSSLVRFTKDIIVEKGMDIKVQILLTSVGAGNLDGSYAARAEIEELKILR"
     gene            33092..33304
                     /locus_tag="PC399_001644"
     CDS             33092..33304
                     /locus_tag="PC399_001644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592506.1"
                     /translation="MKKQVYYNSLDEKEKIIDENSNLYIIEIYETLNENYLVLSSSPA
                     EDKELSYEELENELLIITNELQGGLL"
     gene            33304..33486
                     /locus_tag="PC399_001645"
     CDS             33304..33486
                     /locus_tag="PC399_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592507.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNREFELDDVTNTDYRTAVED
                     YIIKQSGVVEGAEPTK"
     gene            33586..33804
                     /locus_tag="PC399_001646"
     CDS             33586..33804
                     /locus_tag="PC399_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592508.1"
                     /translation="MKKIIVICILVLMLIIPTMGEIFIMVENIKLEGLEQNPTSCYIS
                     NPGIMGEAKCDAAYFIIAADFVNNLKVK"
     gene            33812..34303
                     /locus_tag="PC399_001647"
     CDS             33812..34303
                     /locus_tag="PC399_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2592509.1"
                     /translation="MHLKDLDNSRSFLIQKLGGNMNLTIVFLATNIFIKLVILAIAFD
                     TLLGCLRAIKTHQFNSSFGINGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDF
                     LRLNHLGISEFFSILFILYESVSILKNMYLCGLPVPKKLKDRIGNLLDTMTDELNVKG
                     GSK"
     gene            34303..35160
                     /locus_tag="PC399_001648"
     CDS             34303..35160
                     /locus_tag="PC399_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2592510.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADRFKKE
                     GHKADVIICPEKQFKTKDEEKTYKIPRVNSGGYDLLIELHLNSSGVGAFGTELFYYSE
                     KGKEYAQRVVDKLSKPFIRKKGDKEVGNRGAKLDKSLYILNSSKPTAILIESFFCDNK
                     EDYEKAKKLGHEGIAKLIVEGVLNKSINNEGVKQMYKHTIVYSGDDKVSADILGLYYK
                     REKESYLVTDIKDYKPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDK
                     AIEFVKDKL"
     gene            complement(35234..35416)
                     /locus_tag="PC399_001649"
     CDS             complement(35234..35416)
                     /locus_tag="PC399_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592511.1"
                     /translation="MFNDMIDECIHIMTAYIANLKEYCSFIETQLDDFIKKYGEDIVE
                     SCLHRIMILLCECDLV"
     gene            complement(35717..36034)
                     /locus_tag="PC399_001650"
     CDS             complement(35717..36034)
                     /locus_tag="PC399_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592512.1"
                     /translation="MNSWSIIFLSYGITFTIGTTIRYLIKRNTNYCRKKYLDKLELKY
                     GNINRVKATKLEIFYDYLIGLEYIIMGLLIRRLDTAIISVILVSIVTIISYYLIREKY
                     ITL"
     gene            complement(36432..36632)
                     /locus_tag="PC399_001651"
     CDS             complement(36432..36632)
                     /locus_tag="PC399_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592513.1"
                     /translation="MCFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDIEIDLI
                     IIDEGNSIVWRNEKSTPPQENA"
     gene            complement(36645..36881)
                     /locus_tag="PC399_001652"
     CDS             complement(36645..36881)
                     /locus_tag="PC399_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592514.1"
                     /translation="MSPNFSAENMVLEDKEIISTIEKYFVISRYYDNGKTIAFIEMNS
                     TKYKTEYIRNFDQYVDEFSTLEEAEECLQECLSA"
     gene            37231..37452
                     /locus_tag="PC399_001653"
     CDS             37231..37452
                     /locus_tag="PC399_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592515.1"
                     /translation="MMSISFGEKIKIILKRKNMTIGELAEKTGQTRQNLSNKFSRDNF
                     SEKEIREFAEILDCEFDFYFIIKDTNEKV"
     gene            37471..37773
                     /locus_tag="PC399_001654"
     CDS             37471..37773
                     /locus_tag="PC399_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592516.1"
                     /translation="MEVTLHTGEINFFKDHEVAKNYIGDLYNFIEEYKFSMDTVSILT
                     NIDIKKLNDFYIGKGALHYDELVLINSVVIMPFIDVFKTAKHNYGMIIKSSKKDEE"
     gene            37891..38067
                     /locus_tag="PC399_001655"
     CDS             37891..38067
                     /locus_tag="PC399_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592517.1"
                     /translation="MKTLMFILVIAYYITIVIKSITAIVKSIIRLLDSINRLKESLHK
                     LRNRPDCNQDDFNE"
     gene            38588..>38973
                     /locus_tag="PC399_001656"
     CDS             38588..>38973
                     /locus_tag="PC399_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2592518.1"
                     /translation="MEQQDVIDKLRRGELMLMEYLWNKKSILSKKEIIEAMKKRYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNARKEERDISKYDNFFARIF
                     TTIHKRDKMTEEQLKAFIKNMEKIGK"
BASE COUNT        16085 a         4167 c         7212 g        11509 t
ORIGIN      
        1 gaagttataa aaaatactat aagcgagaag attatagaac gctttaaaaa tttataaggg
       61 gtgaaattat gataatacac aaatttataa tacatgtttt agataagaac agcgatacac
      121 caatactaaa tgattttgaa ggtagagtta gtcaagatat ggacctattt tttcaaaaga
      181 aaataagcaa agtatcaaga gataatgaca tcagaacagc agtatttaat gactatagta
      241 acaatctaat taagaagtgt tgtgaacaaa ttatttatga tgaaagttca tttttaaata
      301 actctaaaga gattgcagct tatttatttg atgttatgaa attgaatgct acattaaaat
      361 cttgcgactt agcaatttgc ttatactctc aaaaagatga aaagaaagtt gctatattaa
      421 aacttgatta taacaagtca tatactcatt caatcgagtt taaagatgat aaatttaaca
      481 tacaaatgtc taaaaatgaa attaatatac aagagactaa gacagtaaaa attgctgctt
      541 tggttggttt gagtggaatg aatgacgaat atcatcttag ggttttagac aaggatgcag
      601 aaaaggaaga agctaattct aagtttatta cagagtttct aaatgctact aaagtgaaag
      661 atgacaagta caagactaag atgtttaaaa gtacagccga aaattggata actaatgctc
      721 ttggaaatga tataaaacag gcagaagatg taagaagtat attaaattat actttgaaag
      781 aaaagcatga aattgatata aataattttg ttgataaaac aattaaagat gataagttaa
      841 aagatagctt taaagaacat atggaagaaa aaggtcttga taaaggattt agtatagata
      901 aaaaatgggt tgagaaaaag cttaaaaaga gaaatataaa aactgacaat ggctttgata
      961 taaaaggtaa tctgactgat tttgaggacc caatgaaata tacagtaaga caaaatcaaa
     1021 atgggtctat agatataatt attaagaatg taaaatttta cgaagaaaag taggtgagca
     1081 tatgactaat aaagaaatgt gcaagtcaaa gaatcttgat gaaagagaaa tatataagga
     1141 atttgggaaa gagatttgtg ctagttgcat aagcgatagg gtagattgcg aaagtaaaga
     1201 ttgtgatata gcatatgaga attggctaga gaaggatgct gaaagataat tataaaaata
     1261 aagtcaaggt aagtttgtga atgaaactag aatgttatag acttactttg acttatagga
     1321 aggaggtgag attatgatta ctactcaaat gtataagata aataatgaga tatttctaaa
     1381 tgatttatgt ttagaagaaa ataaggaaat gaccatatgg gtagaaaaga ataaagatac
     1441 acatgaattt atatggttaa aaatagcaaa tgtaaatggc aagttagcta tatttataca
     1501 agatattgaa aatgcagtaa taaaagaatg gcaaggtcaa atagcatata aaaaagttat
     1561 atatcaaata gaatatgaac aagttgaaaa aggtgaaata gactaaaact tttaaagggt
     1621 gtgagcttat gatacatgaa ttaaaaatat tacctcaata ttttaaagaa gttgtaaatg
     1681 ggaacaaaaa ttttgaagtt aggaagaatg atagaggttt taaaaaaggc gacttgttgg
     1741 tgttgcagga atttgatgga gaaaaataca caggtcttga gatacgcaaa gaaattattt
     1801 atttacttga taatagcaat tatctgcaag atgggtatgt tgttttagga ataaaataaa
     1861 tgttttgtga ctaggaagtg agtttatgaa acgaagaaga tgcagttggt gtggcaagct
     1921 gttttatctt aaggaaaaat ctaaggagat ttattgttgt aaggactgta gaaagaaggc
     1981 taataagaaa aataaatagt ggaggtatta atatgcaaaa agatgtttgg ttatatagct
     2041 gggataacaa atatttaagt agtgatgagt atgaaagtaa agaagaagct atccaagcag
     2101 ctaaagaaga acttaaagag tttggaaatt ttggagaaag tatttatgtt ggtaaaaaag
     2161 aggaagttag tatacctaac atagatatag aagaagcatt agaatgtatt caaaaaaaga
     2221 ttgatgatga agttggagag tgtggggaag attggtttga aaatatatgt gttgaagata
     2281 tgataatact tagtaatagg gtaaacgaag tatttgaaaa atggatagat gaatttggat
     2341 ataaaccata ttggtttaag cttacagata aagaagaaat agaactaaat gaggtagcca
     2401 atgaaagtta attttacaat agatggagaa ccaaaggcga aagcacgtcc tagaatgagt
     2461 acagcaaatg gtaaggctta tacacctgac caaactatat tatatgaaaa ctggattaga
     2521 ctcatgtata actctacagt aaagcatttc tttgagggta atgtgaaaat gacagttatt
     2581 tgttactttg acattactaa aaaagataga gaagcactac agaaaaataa ggtaaatact
     2641 aaagcgtata agaatgctat agacaaggta gaagggttaa taagaccaaa taagaaaccc
     2701 gatttagaca atataattaa gtctgtagct gacagtttaa atggtatagc ttataaagat
     2761 gattctcaaa tagtagaagt agtgagtaag aaatattata gtgacagacc aagagttgag
     2821 gttgaactgg aagatgttag ttaaggagaa tattaaggtt aataatatga aaaaatttat
     2881 ttatataaat aggcatagag tatatataag aaatttattt aaaatatcag ctatatacaa
     2941 agaaaatcat tatattagag agtttaaaag tgaatgttta tctaataata ctttaaatgc
     3001 agtaatagaa tcttctgtaa gaataggatt tggtagtaag taaaaagaaa aaaaggagca
     3061 ttacttcacg ctcctacttg tcaaaaatat aaaactttta tatgcaaata ttattataac
     3121 ataaataatt gataggagtg tgtgagtatg tctaaaacta aaaaagagtt ttttaatgca
     3181 actaagaaac aactttctaa ttataaacaa ttaagtacaa atataataaa actaaaaaat
     3241 gaaatacaaa tgttgaaaga taattcggtt ggggatttaa tgaaagggat aagttatgat
     3301 agtgtcaaaa caggtaaaac aaacaaaact agcaatatga ttgaggatgc tattgttaat
     3361 gtatcagact taataacaga aaaagaaata gagttatatg aagcagaaat aattaaatct
     3421 acaatagatt tagccataag aaatttaaaa cctatacaca gacaaattat tgaacttaaa
     3481 tatatagatg gtctaatgtg gcaagaaatg gttgatatag tacatttaga agaaagacaa
     3541 ttaagtgtaa gagctagtca agctattagc tcaatatcaa tagcactgtt tgggaagaaa
     3601 gcattaatag agcaagaacc actttttgaa ttgttagatt acaaactaaa ttaaaaagta
     3661 agagtaattt tgagtgctga aaatgtgcag gtttttttgt tttagacatg agataatagt
     3721 attgtggaaa taaagatttc cctctcaaaa cttaatattt gactagggtt aagggattgc
     3781 cctagtcact acgaacagac taggcagggc gtgaggacgc tgttagttca attctaacta
     3841 tgttcaaaac ctattaatac actatatgta gtagttgaat taagattaaa atctcataca
     3901 attttgtatc ttaattcaga agtctaaaaa ccgagtgggg cttggtaacc tcactcacca
     3961 tgcaagtact ggtttaatct aggttcgatt cctggaactt gctcccttta ataatatgta
     4021 tcccccatta aaaaggctta gattaacttc taggtctttt ttaatacaaa aatttattaa
     4081 aaatgcacgg catgcactat tcagatatgc atgtcatgca tgtctaaagt cgaatattta
     4141 atattttcga tggctcaaat tgaggggtcg aaaataaaaa caggaggtag tagtatgttg
     4201 aaaattttac aagagaaaaa tgtaaaagta atgtggtcca aaaatggaga agaagtttgg
     4261 tttaatgcaa atgacgtagg agaggaacta ggcatagtaa atattcgtga tacattaaga
     4321 aatatagata gagaatataa aaagaaattt aatgagtcta ctgtcggaga ttcctacact
     4381 agaaacttta aagataaatt gcctaacttc ggtactactt ttgttacaga agaagctgtg
     4441 tacaatatgt catttagaag taataaacca gaagcaaagt tatttacaaa atgggttaca
     4501 aaaacactta aacaaattag aatacatggt tattatattg ctacagaaaa agaccaggaa
     4561 tggctggata taaggacaga aggcaaaaaa gtaagaaaag attttacaga tgaaatacaa
     4621 gagtttgtat attatgctac tagtcaaggt agcaataaac ctcagatgta ttataaacat
     4681 tttactgaac ttgtaagaaa aaaattaggt attccaaaag gtgtgaaaag agatgagtta
     4741 aatcaaagcg aactatttga tatacaagca cttgaaagaa ttatatctat gaaattacct
     4801 aagttaatag ataaagatat gaattataaa gaggtatata aaaagattaa ggaattaata
     4861 gaaatgattt aagggactgt cttgatggag ggtctttttt aattcccaaa acgacaaaca
     4921 aacgaggtgg tgatgtgcaa gatgtcaaag aaaaggtaaa acaagattac ataaaaggta
     4981 tgaaacaaaa ggaaatatca gcaaagtatg acattagttt aaacacttta aagtcatgga
     5041 taaaaagata caattgggct agtgagaaaa agaagggtgc acctaaaaat aaaagaggtg
     5101 cacccatagg taataaaaat gccactggtc ctcctggaaa taaaaatgct gaaaagtttg
     5161 gtttcttctc aaaatatcta cctgaagaaa ctagggaatt aatacaagaa atatctataa
     5221 aagataaatt tgatattctt tgggaacaga taacaattca atatgcagca ataataagag
     5281 cacaaaagat aatgtatgtt aaagacaagg aagaaatgat taaggagtta aagaaacatg
     5341 aaagcacaga aaatggtgag aagatagagt atgaatttca atttgcatgg gataggcaag
     5401 catcttttct taatgcacag agtagggcta tgagtgagtt aaggagttta attaaacagt
     5461 atgatgaaat gattcataag gattggaatt tagctacaga ggagcagaaa aatagagttg
     5521 aaaagttaaa atgtgaagtt gataacctaa agaaaagtga tactggagat gattcaaaaa
     5581 tttgggttga agctatacaa aatattgcaa tgaaacgtgg tgttaacaat ggataaagct
     5641 ttattgacac tattagattg ttattgggat aatcctgttt ggtttgcaga ggatatgtta
     5701 aattttaaag ctgacaagtg gcaatctgat gttctgatgg ctttagctca aaccccaaaa
     5761 gtatctatta gaagtggtca aggagtaggt aaaactggat tagaaagcat tgcaactgta
     5821 tggtatttaa gcactagacc ttttccgaaa gtagttgcta cagctccaac acgacaacaa
     5881 ttatatgacg tactatgggc tgaaatagct aaatggctaa gtaatagcaa ggttgagaag
     5941 ctacttgagt ggactaaaac aaaagtgtat atgaaaggct ttgaagaaag atggtgggct
     6001 acagctagaa cagcagtaaa gcccgagaat atgcaaggtt ttcatgaaga ttatatgtta
     6061 tttgttgttg atgaagcttc gggagttgct gaccccatta tggaagctat attgggaaca
     6121 ttatcaggtg cagaaaataa gcttctttta tgcggaaacc caactagaac gagtggaacg
     6181 ttttacgata gccataatag agacagagat ttatataaaa catttaaagt atcttcttta
     6241 gacagcccta gaacatcaaa agataatatt gaaatgctaa aaagaaagta ccatgaaggt
     6301 tctgaccctt ggcgtgtcag agtacttgga gagtttccaa aaggtgaaag tgattcttta
     6361 atatctttag aagctgttga aacaagcaca ataagagaag tgaatatatc taatgactat
     6421 atattaaata taggggcgga tatagcaaga tatggtgatg atgaaaccat aatagctcca
     6481 agaataggtg ggaaagtatt tgatttatta acttattcaa aaaaagatac aatggaaaca
     6541 gtaggaaata tattaagagc agttgataaa tttaaaaata tgtatcatca aattaacaga
     6601 gtaaaaataa aaacggatga tgatggctta ggtgcaggtg taacagatag attaaaagaa
     6661 gttataagac atgaaagact taaatatgaa gttataccta ttcaaaatgg ttctagtgct
     6721 atagaaaaag ataagtacta taataaagct tctgaaatgt gggataacat gagggaggaa
     6781 ttagatgcaa atttaagtag ttttatacaa aataaagaag ctataataca gcttcctaat
     6841 gatgataaac ttattaaaca actatcaaat agaaaatata cagtagattc aaaagggaaa
     6901 atacaaatag aaagtaaaaa ggaaatgaaa aaaagaattg gagaatcacc cgatagagct
     6961 gatgcagtaa tatattcgtt tgcagaaaat aacaatactg atttatcttt actgaaaggg
     7021 ggtagtgtat ggggataata tcttatgtaa aaaagctatt taaaagacct gcaggagaga
     7081 ttatgcgtat gtctagtgga aacattggcg tatataaatt agacgattct agagttgatt
     7141 atgagttagc aagagaactg tatcaaaata aaaatgctaa ttacaagtta ggttctagtt
     7201 ttgttagacc gattgtcaat tcaacaactg gttttatggg tgtacctcat tttcaaatag
     7261 aagatgaaga agctcaatat atattagatg aatttgtttt agataacaca tctaaaatgt
     7321 taaaaacaca tacagatagt ttaaagcaag gtgattgtta tatttggata actagagaag
     7381 aaagagaaaa tcctttatat cccgataaaa aagttagatt aatatataac ttcatatcac
     7441 ccgaagaagt gaaagaaata atattagacc ctacaacaaa agagcctata gcttatatat
     7501 tagaaagtca aaatgaatgg actgacttag gagaaaacaa gagaaaggct aaggtaaaac
     7561 aaataataac tgctgaaagt agatttgttg aggttgaagg tgataagata gaaggtttag
     7621 aagaagggga aacgcctaat gtatggggtt ttataccaat aatacatttt aaaaatgaag
     7681 ctgatgaaac attgaaatat gggcaaagtg atatagaacc aatagaacct cttttaaaag
     7741 cttatcatga tgttatgtta catgcgttaa aaggtagcaa aatgcactct actccaaaac
     7801 taaagttgaa attaactgat gttgcaagtt ttttagcaca caattttggt gttgaagacc
     7861 cagttaaatt tgccaaagaa ggtggaaaga taaatcttga tgggcatgaa atactattct
     7921 taaacaaaga tgaagaagct gagtttgtag aagtaaaatc agccataggt gatgctaagg
     7981 agcttttaaa gcttcttttt tattgcatag tagatgtatc tgaaacaccc gagtttatat
     8041 ttggagtaca tacacctagt gctttagctt ctgtaaaaga acaaatgcct attatggtaa
     8101 ataagataag aagaaaaaga gaacaattta caaatagctg gcaattactt gcaagaatgg
     8161 ttttaataat gagttctaat tctagtggta tgaaatattc atcttatgat gtgactatag
     8221 gttgggatga agtaaatcca cgagatgata aagaattagc tgaaacacta gaaaaagtat
     8281 gtagtgcatt agataaagct ttagagggtg gatttattag tgaagaatca acagtaaact
     8341 ttttagcaca gtatatagat acaatgagca attatataag tgatgaccct gaaatagttg
     8401 gagaaagaga aaagataata aaaaccaaga tgttaaaata cagattagat gactctcaag
     8461 gtttaaatga tgagtcaaat gaaattgaga aggaaataaa taaaataaag gataataatg
     8521 gcaatggata aaagtacttc ggaattaata actgttgcag gggagtacaa gaaatgggca
     8581 ttagaagcta gaaaaaaatt tatagattta aggctcaagc aagatgatga aataagaaca
     8641 atgtatatta acataacaag aaatattaca aaagaaataa gaaaaggaaa tctttcagac
     8701 tttaacaaag ttaggttaaa acagatacta aaacaattaa cacaagaaat aaaaatatta
     8761 aatgaacaac tagtatttaa ttttgatgaa tacttaaata aaaatgttga aacagctact
     8821 agttactcta aaaatatttt aattaatgca gttgagacag ctcaaataac taaagtaact
     8881 aaaactatga tacaaaaagc tttctatgat attaatataa gaactgtaga agcttattat
     8941 acaagggtta aggatggttt atttttatct gatagaattt ggtctaagtg taagaagtac
     9001 agagaagata tgaaagttat attacaaaca gcagtaacag aaggtcaaga ctgtgttaaa
     9061 acagctaaga tgttagacaa atatgtttta aaaggtaaga aaactttagt tgatgaatat
     9121 ccaaatatga taaaaagaat aggaaataga gtacctcaaa atataagtta tgaagcttta
     9181 agattggcaa gaactgaaat gacatcagct tatggtgatg gggttttagc ttctgcaatg
     9241 attaaccctg caaccatagg tattcagttt atgttgtcca tggcacatcc tcacacagat
     9301 atatgtgacg aaatatgtgg agaggataat tttggtttgg gtaaaggtgt ttatcctata
     9361 aatgaagctc ctgtatatcc attccaccct cattgtttgt gtattatgct tactgtagtt
     9421 caaccattag atatattagt tggaaggttg aaaaattgga ttaaaaatcc tatgaatgat
     9481 gtacctcttg aaatgtggta tcaagaggtg tatggaaatt tgaattttta aattgaaagg
     9541 tggtgattaa atgaatgtaa taactggaga aatggactca atgaatgcgt taatatctag
     9601 tataaaacct tctgatattc ctttagctaa agatatagac atagaagctt taaaatctat
     9661 agatgatgac cctcttgagg tagttgttga gataccagct acaaaatcta aaaggggatg
     9721 gaattatact gctaaaagct tgaaagatat tgtagattac actaatgaaa atactcttaa
     9781 tggcttttta ggacatcaaa aagctgaaaa tatatcaact gaatttgcac tacctgtaac
     9841 gcattggata ggtgcagaaa tgaaagggga taaagcttat ttcagagggc tgattgatgc
     9901 tgatgcaaca aatttaaaaa gatggattag aactaaaagg ataaaagaag ttagtatatt
     9961 tggttatcca aaacttaaaa agagtgctaa aggcgaaatg aatgttatag gatatgagcc
    10021 actatctatt gattggactc ctctacatag accaggtatg ccaacaagta ttgtaggtat
    10081 ggaaatgagt cctaatggcg aacagttaga tggaactttt gaagctttaa gaatagattt
    10141 aagagaagct ttaaaagcta agttttctat taatgataat aattcatatc tctatataca
    10201 aaacataaga tatgataaca atactgtcat atatgagttg gagcaaaatg gattatgcaa
    10261 gctttatagt ataccattta ctatagttga aaataaaata aatctaggtg aagaaattga
    10321 agtaataaag aaaataagct atgaagctaa aggagaaatg aaaggagagg aaaacaaatt
    10381 ggaaggaaaa gagttaataa aaaatgtcaa aggattactg caaactggtg aaatatcata
    10441 ttcagaggtc atacaaggaa taggcttaac taaggaaatt gtgacaggag agatggaaga
    10501 tgtaaaaagt tcattaaaag cagaaaaaga attaagagaa gtgaaaaaag tacttggaat
    10561 agtaggagag atggacacag ttgaagtggc aaaaaaggct tcaaaagctt tagaaaatga
    10621 gaaaaaggaa gcttggaact gtatagttaa taaagtaatt aaagataaag tgtcaggtga
    10681 aatagctcaa acattagtta agaaaatgtt aaatgttgag gaaggctcaa gtgaagaagt
    10741 aataacagga gaaatagaaa atatattaaa tgatgagttt gtaaaaaata caatgtctaa
    10801 tatgtataaa gataatccaa caacaacagg attattaaac tctagcaata atggaagttt
    10861 aacaactaag aaaaatagaa tataaaggag tgatgtttat atggcattta aaggtcaacc
    10921 aacgccaagc acaataacac agataacaag agcaaaaata agtgatggga aatctgtaag
    10981 agttattctt tcagaaggtg aaagcactaa aacacaacaa ttttatctta taaatggatt
    11041 ctttggagtc gctatgcaag acggagaaaa aggcgatgaa gttactttgc aaatagagca
    11101 agctgaatac gaaacggata atattgttac atcagaagct tttgaggcag ggaaattgat
    11161 ttattgggat aatacagcta agaaatttac tactacatct gcaagtaata ggctagttgg
    11221 tagagtaaca gatgggaaag acagtaataa tgtaatttgg tttatattat tacctcaaca
    11281 atagaaaagg agtgataaat atatggcatt taaagtaatt agtcaggaaa atttgctgga
    11341 acaaaaaaga aaagaaactt tacaagaaga tataccattt atagtaaatg gtgaaatgga
    11401 atatgtaaca aagaaaatat caaatggaga aatggaaacc ttggagttaa ataagccact
    11461 tggtgaaatg atgactttta gctcgacttc aaatttaaaa gagttattaa gaaaagttgt
    11521 attagatgtt gaactaggca gagagcaagt acaactatta tataaaccaa tctatgacag
    11581 tatagcagat tctaatttac cacaagttat ggatgctaag tgggctttac aaggtaactg
    11641 tgtattccta gagcatatag aaggtgaaga aattaaattc ggtacaataa atgcagaaaa
    11701 tggtccagtt gcaaggatac aaacttatgc aactggtttt gagtatacaa aagaaatgaa
    11761 ggattttaac caaacattta gtgttgaaat attaaataaa tcaattggtg agagttacaa
    11821 tgccttgtta aaccacatac atctaagccc aataataaat tttaattata aagcttctaa
    11881 taagacagct tttaaaggtg aaactaatga cccaatatgg ctaggaattt ggagaacatt
    11941 aacacaagca caaaaagata cagttatagc aaaaagacaa ggtaatatat taatggcttc
    12001 tagtgctgac caaattgaaa tagaaatggc gttaaatgga ggacatttat taaacggaag
    12061 catgtatcca tctataaaaa atatatcaac agtaatttat tatgatgggt gggaggttac
    12121 tgttggtaaa aaaacatatt cttacaaagg tgttacacca ggcaaaggat atttgataag
    12181 acctaagcga ggatttaaag agttaataaa gagagattta acaacagagg ttggaaatgc
    12241 tgatttaagt aagttagtag aaaatcaaat tgtaggtcat tgttatagag gtgcttttgc
    12301 agcagtagaa gaaaatgtac aagaaataag ttttagataa aacactcata agagtgttat
    12361 ttttatgagg tgataatata tgacaccagc tagagattta atagaaaaat taagactatt
    12421 attaaatgat aaagataaaa aatcatttac agatgaagaa ttaaacttgt ttttagagga
    12481 agcagactgt atttactgtg cagcttctca aggatggata ttaaaatctt tacaatatga
    12541 aaatacagta ggggaaatgt atgagtataa agtgggtcaa gaaacatata aaagctctag
    12601 tataaaagac ctagtatctg tagcttatca aaatgcagat aaatttaagg atatgtgtac
    12661 taacaaaaaa gaaaagggaa gttttatgtt aggaattagc acagaatttg aaatatgata
    12721 aatattgata gaagaagaaa agatataata agaactatta atataaaccc cactaatatt
    12781 actataacta gtattaaaaa aaccgaaata gatggagctt ttgaagaaac tgaaacagaa
    12841 ataaaatgtg ttgttagaat atttaacgaa aagacagcag agaagcaaat atcaagtgaa
    12901 aagcaaggta catttagttc tattagaaca tatggaatgt tagtaagtaa tgatgttatc
    12961 ttagaggtta acagtagaga ttctttagag tttgagtgca tatatgggag aatgaaaata
    13021 gttaatatat atcctcaaat tgtaaaagga gaactttgtg gatatcaatg ttcacttgaa
    13081 aggattgatt aaaatgagtg ctttcacaaa tgcaataaat gatataaata gaaaaaaagc
    13141 aggtatgctt gtactttgta tgagtgcaag tgcaatgcta gaaggtgaag ctaaagcaaa
    13201 tgcacgttgg acagatagaa catcacatgc aagacaaagt ttaaatgcta aaacacttag
    13261 tggaggaaat aattttatca ttagattatc tcatggtgca gaatatggag ggatacttga
    13321 agaaggctca aaaccacatg ttattactcc aaaatcagct caagccctat actggagagg
    13381 tgcttcacat cctgtaaaat cagttcaaca tcctggtaca aaagcaacgc ctattataaa
    13441 accaactatt gataaaaata taggtaaaat aggtaatatg atttttaggt attggagtga
    13501 ttaaatgagg gcaggaataa gaaaagcctt aatagataat ataaaagaat tgaaaggttg
    13561 ttatgaacct aatgtaccaa acaaagatac taaaaaacct tatatggtag ttgtacaagg
    13621 gcaagacaat gaccatggag aaacaatagg ttttgaaaga agtatagaag tatggattta
    13681 tgaaggtaga acaacattca agaaattaga taaattaact aaacaagttg ttgaagtctt
    13741 agatatgaat actatagttg atgaatctga aaacgaagct tttacttgca tttataaagg
    13801 tacaagtgaa aatgatattg ttgttgagga atgggatgct atagcaagag gtataaggtt
    13861 tagtgtaata gctttagaag ataaagaaga tacaactaat gataggtggg tagaagctct
    13921 atctaggcac acaaaggatt tattagaaat agagagttat aaagataatt ggaagaaaaa
    13981 ctttatagct ccatgtgcat tatggcgaac tacacatatt gaaaataaaa gaattaacta
    14041 tcatttaatt gagattacta aaactatgaa atgtcatgtt gtaagtaaaa ataaagatga
    14101 aatagttaag cttcttgaaa cattagaaac aagcttaata atagataaaa gagtaagact
    14161 tagagaagat aagaatatgt atttaactct tgttagcgta gttgaggata gggaatcaga
    14221 tatgtttaca actggacaat taacagctgt gtttaaaatg ataggaaaga taaaaagaga
    14281 aggtcctact atggataaaa tttatggtaa tggaaattta aaataggagg tgcaagaatt
    14341 ggctgaaaca aataataaaa agattaatgt aagtaagcaa gaagaaaaat atttgaaaag
    14401 tgattttata aaaaatagcg aagcacttgg ctacggaaaa atggtagttg caggtgcttt
    14461 atttaattgt aagaaagaag aacttacaaa agcagaattt gagaaattaa taaaagattt
    14521 cttagaaaga gaggtgaaat aaaatggcaa ctggtacatg gaatgaaaaa gaaagaaaag
    14581 agatacctgg tttttataac aggttcaaga ctcaagcaga aaaatctaca aacacaggat
    14641 taaagggtag attagcaatg cctattaggg ctaattgggg agacgttggc aaggttgtaa
    14701 caataaaaaa tgatttaaga cagcttaaaa atctatttgg ggatgatatg aattattcag
    14761 ctttcaagct aggcaaattg gctctgttag ggaatgtaaa agagttatta ctatatagac
    14821 ttgtagatgg aaatcaaaag aagggtacat taacactaaa agatactaca gaaaatagtg
    14881 caaaagatgt aattaagtta gaaactaagt atccaacagc tagaaacttt aatgtaacaa
    14941 taaaatccaa tttagtagat tcagataaaa aggactttat attctttgaa aatactaaac
    15001 agttatttag ttcaagtatt aaaggcacta tagatgaaat agtactagaa ataaactcaa
    15061 atttagataa tgagtatgta attgcaacta aagtagctga tagcgataca attctagcaa
    15121 atgtagtaaa tcaagcttta gagggtggga atgatggttg cacatctatt actaatgagt
    15181 cttatctaaa agcactagaa gaatttgaaa gatatagttt tgactctttt gtacttgatg
    15241 gtgtggctga tgaagcattg caggaaacta caaaagcttg ggtagctaaa aataaagaat
    15301 taggaaaaga tatactactt tttctaggtg gaaaaacaga ggataatata aaacagataa
    15361 atgataaatc aaaaagtttc aatgatgaaa atatagttaa cgttggaagc tcagcttatt
    15421 atgaaaatat aaaatataca cctagtgaag tagctgttta cattgctgct ctttctgtaa
    15481 gtaaaggtat aacgggtagt atatgtaatg caaaaactat atttgaagaa gtagaaccac
    15541 gattaagtca atcagaagtt aaagagtgtt tgaaaagtgg tacattggtc ttagattttg
    15601 atgatggaga tgtgattata gttgatgatg tgaacacatt taaaaaatat gtagatgata
    15661 aaaacgaagc aatgggatat atctctaata tcatgtttat taatactata aataaagata
    15721 cttcattaaa aagaaaagag tttgtaggta agatatttaa tgatgcaaca ggtcaaacaa
    15781 ctgttatatg tgcattgaag aaatattttg aagaattgat gagtcaaggt attatatcag
    15841 aatttaatgt tgatatagat acagagcttc aagcaactgc caaagcagat gaattttact
    15901 ggaagtggga tgctgttaag gttgatgtca tgaaaaaaat atatggtact ggatatctag
    15961 gataaaggag gttataaagt atgggaaaat atgatgaaaa tattatagat gctgcaaatg
    16021 ttgttgatgg ttcaaatgct agaataataa ttgatggaga agaagaagga tatggaacag
    16081 aatttacagc tgaggtagaa aatgataaaa agacttttag agtaattggt tgtaaatggg
    16141 aacttaacaa ggcatccact caaaaaggaa ctttttcctt gactgtactt aaaactacat
    16201 ctaagtggat taaaaaagga tttaataaat ttgaaataat tacagaaata gaaaatcctg
    16261 gattagttgg atatgaaaga attagatata aaaattgtat ggtagataaa atacaactag
    16321 caagcataaa atctgatgaa aatatagaaa tacaaataga tggaactttt gaaggatttg
    16381 agttgttaga tgaaatagca taataaataa atttaagcta cacgtaatta attttatgtg
    16441 tagcttttta taaaactata aaattggagg aagttaaaaa tggaaaactt agataaagaa
    16501 tttttaaatg aaggaataga agaagaaaga gagcttacta aagatgaaat agcaaagcag
    16561 caagaagata atataattat gaaattgaca gaggatgcta tattacctga aaaaactatt
    16621 tttgtaaaaa ggttagatat accacttacg ctgagggctt taacagaaaa agagataagt
    16681 gcattgcaga aaaaatatac aaaagttact aaggtaagag gtagaagaga aagtaaacta
    16741 atggaagatg aatttaatat agctctaata gaaaaagcta caatagttcc taattttggt
    16801 gatgcaagac ttcttaattc tatgaaggta tcaaatggag tggaatttat aagaagaaag
    16861 tttttagcag gtgaaattgc attaattagt gatgaagtac tagaattgtc tggattttat
    16921 gaagaattaa gtgatggtga tataaaaaac taataaagag aggtgggaag attactattt
    16981 tatataacgc atatgttaaa catagtgttc ttccagaaga ttttctaaaa agagagaaaa
    17041 cacctcaaca gcttcttaga gtttttactc aatatgaaat agaacaagaa aataaagcta
    17101 tgaaaaataa atgaaattta aactgaaagt gaggtgagag aaatagctaa aaaagaaatg
    17161 tatcatattg atgtcgttat tgatgttaca ggagatgaac aaactaagag taaattaagt
    17221 gctatggaaa gatacacgaa acagacagaa aagagaatga aagcactgaa taggataaaa
    17281 gctaatccaa ttatacaagc tcaagataaa acatctagtg ttgtaaatag aattagcaac
    17341 aacttaaaaa gagtgggtag aactatatct acaaccataa acgcaaaaga tagagcatct
    17401 agcgttgtaa atagagttaa aaacaaagta aatagcttac ttacaagtcg acaaagagaa
    17461 gttttattaa aggctagaga caaagctagt caagtcgtag ataaagtaaa ggcaaaggta
    17521 caaaatctga ctgcggctac aataattagt ttaaatatga aagctgaccc agcgctaaga
    17581 gttatttctc aaactagaag taagttagga gagctcaaaa acaacacaat aataaatatt
    17641 aaagcaaaag gtgaagaagc attaaatact atttctcgta ctaagagtaa attacaagag
    17701 ttttctaata gaacttacca agcaattgta aaactaaaag atgaagctag tccaactttg
    17761 agtggtattg gaagtaaaat agattcattt ataagtggag caataagtaa attcgctcaa
    17821 ttaacaacag caattactgt tgcacttgga ggtatagggg ttggttcttc gataaaaact
    17881 tttgctaatt tcgagcaagg aatgaaaaac gttcaagctg tcacaggagc aagcggaaaa
    17941 gaaatggaag ctttaactgt aaaagctaga aatcttggga aaacaactgc atattcagct
    18001 agagaagtga gcgatgccat gtattatgcg ggtatggcag gttggaaaac taatcagatt
    18061 atcgaaggta tgccaggcat cttgaattta gcagcaacag gaggaacaga tttggcacta
    18121 acgagtgaca ttgtgactga aatacattgg tcggttatgg agaaatctat agcataaaac
    18181 attcggcaaa atcggtagat tctaagttct aattgataat ttatagtata atatttatag
    18241 ggatagatta ggaagtcgcg agcctaattg aaaaggagta tttccgagac ttcttccctt
    18301 ttataaatta agtaggaaaa atactgcgga ggtatttaaa aatgaaaaat ctatgtaaaa
    18361 attgtgggaa agaatttaat ggaaaggtta atcagaagtg ttgttcaaga aaatgttcca
    18421 atgaattaaa gtttccaaaa gtaataacaa aatgtgatta ttgtggcaaa aaaatagaaa
    18481 aaagaccata tgagatagaa aggtcaaagc accattattg ttcatctgaa tgcaggttta
    18541 aacaccaaaa gctaacatta aaaggagaaa gtaatcctaa ctttaaaaaa gctaatattg
    18601 atgttaagtg tagtaattgt ggaaaaaaaa ttaatatact taaatgtact attaaaaatt
    18661 ctgatggtac aaccaaaaaa aacttttatt gttctcagat atgtaaagca gaacatcaaa
    18721 agaaaattct aaaaaaagaa agcaatccaa attttaaagg tggagatatt aattgtagct
    18781 gtgataattg tggaaaagaa ataaatgtta agagacataa atataaaatt cataaatatc
    18841 attactgttc acaagattgt aaagctaaac atcaacaagt aattttagta gggaaaaata
    18901 atccatttta caaattggat ttaagtgaag aagatagagt gaaattaaga tgcatagatg
    18961 ggtataacac atggagaaga cttgtatatg aaagggataa ttacacttgt caatgttgta
    19021 gtgatgataa aggtggaaat ttagaagccc atcatttaga tggttataat tggtgcaaag
    19081 agaaaagaac taatgtaaat aatggagtaa ctttatgtga gatatgccat aataagtttc
    19141 acaatttata tggatttgga aataataaga aagaacaatt tgaagaattt atgaaaaata
    19201 acaagaatca agcatcttaa attttaagat gctttttatt atactaaagt tttttaataa
    19261 gaatatgaaa gtaccgaggt aatattacag attgcgaaag gttgtaatac accgtagagc
    19321 gtaggagttg aataaatata atactcccaa gagtgccgaa caccctaacg aattagacga
    19381 gggtgaaaat gtacgccaaa ctaggcttga aaagacaagc cgatgaaaat gagggaaact
    19441 tctagagtct aagataaaaa gcttagagat aataactatt ggatggacta actgcgttag
    19501 gaatgactgc taaagatact ggtcaattcg ttgatattat ggctgcaact ataacaaatt
    19561 ctaatacaaa tgttgagtta tttggggaaa cacttaaatt tattgggtct ctaggaggtt
    19621 ctttgggtgt acctatgagg gacgtggctc tagcaacagg tttgatggca agtagtgcta
    19681 tcaagggaag ccaagcagga acttctctaa gaatgggtct tttaagactt ataaatgagc
    19741 ctaagagagc tgcaacagct atgagaaaat atggaataga aatgaaaaca actaaaagtg
    19801 gaagtttaga tttagcagca acaatagatg aacttagaaa taagcttggt aagcttagtg
    19861 acacgaagaa agtaggagct ttaggagata tagtaggagc taatgcttca agtggttggg
    19921 ctgcaatagt aaatgctagt gaaagtgact ataataaact aaaaaaagcc atagctgaaa
    19981 gcgaggaaga agctaaaaga atagctgaca tgaagatgga ttccttgaat gggcagttcg
    20041 ctaaattaaa aaatactata aatgatgtaa aaattagtat aggagaaaaa ttaggtccag
    20101 taacaagaaa ctttatggaa aatataatta gtagtatgcc taaagttggt gattcaattg
    20161 taaattttgt gagtaatttt ataaataact ttgataaaat aaaaaatgtt ttacaaggtg
    20221 tggcttctgt tgttggtggt gttattgctg gatttatggc ttttaaagct ttaacagtaa
    20281 tttctagcgt aataagtata attacaaaga tagctacagc atcaacgcta attggaggaa
    20341 tagttggagt tcttggaccc gttggtgcaa tagttttagc tataggcgta ttagcaggag
    20401 catttttatt tgcttatcaa aaatctgaaa cttttaggta tggtattaaa aacataggta
    20461 aatcaatatc taatttttta aaacctttaa atccttttat agaacaaata aaaggtaaat
    20521 ttaaagaact tataaatgcg ttaaaacctt tgtttgcagt tttttctaaa ttaggctcaa
    20581 tgattgtaag tgctttatca ccagttatac aatttttggc gacagttttc ataataaaat
    20641 ttatgtattc atttaatgta attgttaaca aggtaaaagc tatagtaaat acaacaacag
    20701 tagtagtaaa tggtttaata gagatattta aagggatagc tgatgtagtt aaaggtttta
    20761 taaatagaga tatgagccaa gtagtcaacg ggcttaaatc tatttttaaa gggattatag
    20821 atattgttaa gtcattgtgg agtgggttag tagattttgt gacatcacca attcaagctg
    20881 tagtcgatat tttggatact aagtttggca aaaaagtgga aggcataaag aaaaaatgga
    20941 atgaattaaa agacttttta aaaaatccta ctaaatcagc tccaaaagtt cagcctgtta
    21001 atttatctag cgagaaagca tcaagcgaac tgcaaacttc atcaaatgga gcaaaagaat
    21061 atataagctc attaggtcaa aaaataggag aaggtattgg gaaaattaaa gagaaatttg
    21121 gagaactcaa aacatctgcg acagaagtat ttaataatat agtaactttt ataggtggca
    21181 aagcaacaga attaaaagat aaactattag aaggcataaa acctgctata gatacattta
    21241 aacaagcttt ttctaatctt aaagaaactt ttgggggctc tttggacagt ataaaagaag
    21301 cttttggagg tttaaaaact gtatttgatg aaaatattaa aacgcctttc gaaaatttaa
    21361 aacaaaaagt tttagaaaca aaagaaagtt taaaaccagt ttttgataac ttaaaatcta
    21421 gctttgcaga actaggaaaa gctcttgaac caataaaaga agcatttagt ggaataaaag
    21481 atttcttttc aaatttgttt aagccaatca aagatgatgg agcaactaag acaactaaaa
    21541 ctaatatgga tgagttaaaa caatcaacac aaagcgtagg aacgtctttc aaagagctag
    21601 ggaatgcttt taatcaatta aaagaagcag caaaaccttt tatagactat ttaaaacaga
    21661 taaaagattc tttaacatct actctgggag atataggggg aggattactc aaaggtgtag
    21721 caacttctat agttttagtt ataacttctg ttattaatgc aattgcatct attataaatg
    21781 ctgtagcagg ggctgtaaaa ggtgtaattg acatagtaaa aggaatattt gaagttatag
    21841 gagggatagt tactggtgat ggtgaaaaaa taaaacaagg tttctctgat attttcaaag
    21901 gtattgggga agtggttaag tctttatggg aaggtataaa aggagttttg ggagcaccac
    21961 ttaaagctgt tgtagatttt gtaagtaatg gattttcaga aaaagtaggg caagtaaaac
    22021 aatggtggtc tgatttaaaa actaatgtag gccaaaaaat aagcggattt gttagttttg
    22081 taagcaatgg ttttcaacaa aaagttcaac aagttggaat gtggtggcaa ggacttaaag
    22141 taaatttatc tggcaaaata agtggatttg taagtcttgt agaaaacgga tttaaaagta
    22201 aagtggattc aattaaatct gcttgggatt ctcttaaaaa gaaactttct accaaaataa
    22261 ctggttttgt aagtatagta aaaactggag taagcaatat actagaccat tttgcagatg
    22321 gtggtgttgc aagtaaacca tccatatgtg gagaagcagg tcccgaaatg gttattcctc
    22381 tttctaatag taaaagaagt agagcattaa gtttatatga acaagcagga cagatacttg
    22441 gaactaaagc aagtaataat gttattccaa tttctcagaa attaggaaat agctttaatt
    22501 ctacaagtag tattcaaaag agtacttcta atattattga taacattagg caatttcctg
    22561 ctagacaaga agaatttaat aatacagaaa atagaattta tcaagaagct caactacaaa
    22621 acataatttc tagtggaagt aatgcgatta atgttggtgg aatatctata aatattcaag
    22681 acaacaataa caaagaagaa atgatacaag aaatattgtc tcaagtggaa agtgagctaa
    22741 gagaagcatt acaagatatt ggataatgtc gaattattgt taaaaaaatc ctccttatag
    22801 atgttataat aacattatag actattgatt tatgagggga ttaaaatgaa tttttttaaa
    22861 cgaaaagatg acataaagaa ttttaagctt atgtatataa aagggcaccc acattttaac
    22921 aatgaactta catgtactat gtatatagat aaaaaactag tatctgtata tacattgcaa
    22981 caagtaccta ttagtgaaat aaaactttta gatatagaga aagtctttat tgataatgat
    23041 atgctaatta tagaaagcaa aaaaagtgta ttgtatttta aaagtaactc aaaagataaa
    23101 atatgcaata taattaatca aaatatagca gatatgcaag ataaaaagac agatactata
    23161 ttaagaaata aaaataaaat agttaaacaa ggggtaatga agatggatta ctataattct
    23221 gttggagttc ctaattgtag gggtgaagca ataagtataa ttattgacaa ccataatgca
    23281 tgtttagtta ttgagagcaa acttttaaaa gaagaaaaaa tgcgtattaa attttctgat
    23341 ttatatagtg taaataagta tagtgagaaa gaaatagcat ataataataa gtctgttgtt
    23401 ggaagagcag ctataggaac tttatttggt cctatgggaa caattgttgg tgggttgtca
    23461 ggaactggaa ataaaaaaag taaaactgaa aagttgttta taagtattga atatttttct
    23521 catggagaat taagggaaat aatagctgaa gattcaatac ttaattttca tagccaagaa
    23581 ttcattgata atataaggag aaaaataaat ataagtaaga ataatttaga aaataataaa
    23641 gaaaaacaaa taaaatcaaa tgatgatgca atacaacaag tgaaagggtt aaaagagtta
    23701 ttagacatgg gagcaataac agaggaagaa tttaataaga agaaaaaaga attattgaat
    23761 ttataacaag agaaaataaa taagtataaa catacaaagc acttggatat tctgttgttt
    23821 caagtgcttt atatgttaaa aaatggtata atataggtaa ggctttagca gtgttcgatt
    23881 ttttgaaata ttgcttgtag tgattgaaat tactatatta caggtatgct ttatgaaatg
    23941 ccaaaaaaca ctactggtcg ctcactgcaa atttaagaaa gctatatata tgtaagtatt
    24001 ggaaatacta aatttatttt gggattttat attaactatg tggtatgtaa atgcaaaagc
    24061 tgttattgca aaagttatat tacttagcag attttatatt aactatgtgg tatgtaaatc
    24121 gttagaatta atcaaattca ttttcaactc actaaatttt atattaacta tgtggtatgt
    24181 aaataagcag atgatgcttg tgagaaaata gttcttaatg tattttatat taactatgtg
    24241 gtatgtaaat aaggctttag agactgtaag aagtatattt gctcaagatt ttatattaac
    24301 tatgtggtat gtaaatgcta aaacattact tgataaaaac aaaattacag gattttatat
    24361 taactgagtg gtatgtaaat ttatcaatct tagtattaga tgtatctttg tataattgtt
    24421 ttatattaac tatgtggaaa aaaataaata aaaaaagaaa gcacttactt ttggtaggtg
    24481 cttttgtttt gctcaaatta cctaattcta aaatcggttt aactgacttt agaacgtaca
    24541 aatagtaaaa ttaactaact tagaagttgt taagtacaac ttttgagaat tatacaatat
    24601 aaaaatgaac taaagtgatt gattttactc aagttggtcg gttgagtgaa ataattagaa
    24661 aaaattggaa taagttattg acttttgtct gccaaaacta tataatataa ttaaggcaga
    24721 caaaagtgag gtgaagatat gccaagtaaa aaaataggta gacctacaga taatcctaaa
    24781 ggcagtagaa taactataag attggatgaa gaatctaaaa atattctaga aaaatactgt
    24841 gaaaaagaag atatagacaa agccgaagga gttagaagag gtataaaact actaaaagac
    24901 aaataaaaag tagcccaacc gccgaccaaa gcaaaatgga ctacttacac cagaagttac
    24961 cttctatgaa atattttatc atagtaaggt acttcttaca atcaaattta aggaggaatt
    25021 tactatgaat gaattaatga attttgaagg aaaagatata gaaatatttg aatataatgg
    25081 gcaagtttta tttaatccat acgactgtgg aagatgttta gagctaagtg atagtgcaat
    25141 aagaaatcat ttatctaaaa tgaatgatac tcaagctatc ttgttaaaaa attcagctgt
    25201 cctagataag gacatccgaa aactgaataa tgcaggtgaa aagttcttaa ctgagagtgg
    25261 ggtatacaag ttaatattta aatctcagaa agaagaagca gagaagttcc aagactggat
    25321 aagcgatgaa gtacttccaa acattcgaca aacaggtgca tacataacta acaatgccaa
    25381 ccccgaaaaa ctaagagaaa aagcaagcga gattgaaaaa ctacaactag cttataacag
    25441 tacatctatg ttaaaagaac tattagatga tgcaggattt gacaacaaat caaaactatt
    25501 aacagctaaa acattatata aaaaggcagg tattgattta ccgattgaga taaacgaaga
    25561 agaacattat tttgatacaa aacaaatagc gtctaaactg aaaatatatt ctaagagtaa
    25621 taaaccagct cagatggctg tttgtgaaat tattaaaaag attgatttag aagaaaatga
    25681 agtaaaagga gtttgggaaa caaatggttc ttggactggt actgtaaata aatatacaaa
    25741 gagtgtaata gacaagatta gcaaatggat agaggaaaat aatagaccta ctaagataca
    25801 aggtgagaag aagaatttcc atgttgtata taagattgag tagatttatc agttgtatta
    25861 aataatatat tttattttat tttggggggt aatacaatgt gtgagaattt acttgatagt
    25921 atagatattg aaaagagaaa agaagaatgt agaattaaac ttttgaaaat aagagaaaca
    25981 gatatagata tatataataa gatagaaagt atagtatata aactttctga gaaaaaatta
    26041 gagaaaaata agtaaataat atggataaag cacttggata tttcactgtt tcaagtgctt
    26101 tatatggtaa aaaatggtat aatgtagata gaagttttgc agtgtgcgat ttttgtgata
    26161 aagtaggttt ttagcagttg caatgtaagg tgttgagggt gtatgataag tgttattatt
    26221 tgcactacta cccgctcact gcaaatttaa gagagttata tatatgtaag tcttgaaaat
    26281 actaagttta ttttggggtt ttagattaac tatatggaat gtaaatattt ctaaagcttg
    26341 tacagggctt gtattttgtt tgagttttag attaactata tggaatgtaa attaatatat
    26401 gacttataac tttaattttt tgattttacg ttttagatta actatacgga cttaaaatta
    26461 aaaataatta aaaaacactt acttgaatag taggtgtttt tttaatgaaa ggatgtgatt
    26521 atgatgtaaa aagtaataaa taggtaaaat atgtaagaat tatgtgctat aataattgta
    26581 gcaagaagat gtaatctaca atttatagag tggagttcat acaaaagatt atcctcccaa
    26641 cgtatagaag ggaggtgtgt atgtatggat aattttttga ttggcgtatt agctagctta
    26701 acagctactc taatagctta cgtaattggt aaattaatca aaaaagcaaa aagccactct
    26761 cgtgcaaaga gtgacctaat agttgaattt aaatttatgt ttaaatccaa gaaatgattg
    26821 agttttaatg aactccactc tacgactaaa tagattgtag ttcttcttgc ttttattata
    26881 ccacaaatta gaaaaatatt acctataata ttttttatag tcaataaaaa gatgaaattt
    26941 tttatacaaa ataataatga atctaaatta atgaataaac ttaattgtaa aacggtaaat
    27001 acagtaagca cttacaaaca tgtaggtgtt tttttataca aaaaatcaaa ggaagtgaat
    27061 atatggtaag aaaactgcgg tgtaatgtta aaaaatactt tagaaaggaa gtgataactt
    27121 ggtaatagat atttatctaa aaaatgaaaa agaaaaaata gatttccatt ttccagtaaa
    27181 tccacaagat tctctatcta ttaaaaaaga aaaaaagttt gaaactgtag atatagtaaa
    27241 tttaggcgaa tttgacatta aaaaagaagg cgagaagata agagaaatat catttaaaac
    27301 atttctacct tttcaatatg atgcttctta ttgcagatac agcgagttaa aaaatccaat
    27361 cgaagtagtg gcaatgcttg aaaaatgggt agaccaagcc gaacctttaa gacttattat
    27421 aactggtttt ggctacaatg gattagtcac aatatctagc tttagtaata cccaaacagc
    27481 aggaagagaa gaagacagag acattgagat aacatttaga acttacagag aactgaagat
    27541 agagacatta aaaaaagaaa ctaaaagtaa tactaaaaca gatttaaaag ataatagacc
    27601 taatacccaa actaaatcta aaatatatac tgttaaagca agtgatacat tatataagat
    27661 agctaaaaat cttttaggta agggttcaag gtggccagag atttataata tacccgaaaa
    27721 caaaaaagtc attggtaaaa atcctaatat aattaaaaaa ggacaaaagt tggtgatacc
    27781 ttctaaatga aaataatatt aaatggaaaa tatgatattg caaattttaa cgagggaata
    27841 acattaagtg aagctataga cggagttgca tataagatgg atgtatcatt agtagaacct
    27901 aaacaactta aagatataaa tattaaaaaa ggtgataaaa taattctaat tgatatagca
    27961 tatgagagta aaaaagaaga gacaatattt gatggagttg tatgggaaac tagaaggagt
    28021 gaaaagagta agaaactaac attgtcttgc agagaaagaa cagtttacat ggaagaatca
    28081 gaggaacaat atcagtttaa agaaaataca gcaacacaga gaattgaata ttactgtaag
    28141 caatggaata taccctatta caacttagct aatacaggga agaaacttgc taaagtaata
    28201 cataagacaa atatactaga tatgataaaa aaggacttaa aagaaacagc aacaaaaggt
    28261 ggagacttat ttagagtaag gatggataat aaattaaaat tattcaagct tggtactaat
    28321 gcaaatgtat ataaattaga tagtatatta gaagatgcta actttacaag tagttttaat
    28381 gatgcagtaa caagtgtaaa agttttaggt aagagtaaag acgaaaatac aaaagcgcct
    28441 ataattggga catataaaaa agatgctgat aagtttggaa cactacaaaa gattaaacaa
    28501 gatgaaaaga taaaaaatgc taaagaagct aagaaagcag cagaagcaat gttcaatagt
    28561 ggagaggaaa caataagtgt agattgtgca gtagatataa atagaataag agcaggtgac
    28621 aaagtaagtt taaaaagtaa agaatattat gttatagatg tcactcatac actagattct
    28681 agaccgaaaa tgaagctgaa tatagggtct ttagaatata taaggaggaa attttataat
    28741 aatgactgat gctagattta atggaattgc tagaatattg aaagaaaata tgaataaaag
    28801 tgtagcaaat ggcacttttg gaatgggttg tgaacttgca gaaataacag caaatggatt
    28861 aaaagtaaat ggctataaag atgaaataca ggactatcta gtattagaga atttaacatt
    28921 aaaagaagat tattttactt tttcagatga agctttaagt ggagaatata gacataagca
    28981 taaaatagaa actccaaagg aattgaagcc actacgtata ggcgataatg tgctagtagc
    29041 tgttatggga gctgaatttg tagtaattgg gagggttgta aatgccaaac ctatttcctc
    29101 aaagtgaaac ttttgaaact gtagaattaa aaaataatga tgaaaatgaa ttggacctaa
    29161 agggttcttt tttatttgat tttgaaaaag gtgaatttgt taaaaacgca gatggaacac
    29221 taaaaaaatg tgataaggtg caggcgtaca aacaatggtg tcaaaaggct atattaacac
    29281 ctagatacaa aaaagcagct tatacaaaca tttatggaag tgaaataaaa gacttaatag
    29341 ctagtaactt atctcaaagt gcaaaagagc ttgaaataac tagattaata aaagaaacta
    29401 ttttggttca tccttacaca aaagaagtag gagagtttag ctttaattgg ttggagaata
    29461 gcaggttagt agagtatgaa tttgatgtac taacaataga tgatgaaaat atagtaattg
    29521 atggcaatat aaaaaggtag gtgattatat ggaaagagag ctacctatac cagtattttt
    29581 aacagaagat gaggactctg tacatgaaag gatgttaagc aactttcaag atgtttctac
    29641 attagaaggt gacttcatct atgatgcaac aagacctaca gcagagcaga tagctgaatt
    29701 aaaacaacta ggattacaaa ataatttaaa gatagcattt cctcagactt cttatggaac
    29761 ttatttagag tggcttggtg aatgtaaagg agtatttaaa aatcaaccaa ctaaggctac
    29821 tggagttatt acatttacag gtgtacaagg aactatcatt acaaaaggaa ctatagtaac
    29881 tactattgca actgatgaaa aacagagcat agaatttgag cttcttgaaa ccaaaactat
    29941 aggagaaaat gaaacagtag atattaaagc agaaagtagg attgtaggaa ctatagggaa
    30001 tgtgtctaaa ggtagtatat ccgttttact aggttctatt agtggtgtta aatcaattac
    30061 taataaagaa gatttcagag gtggaacaga tatagaagat gaagaacatt ttagagaaag
    30121 agttcttgta gcagagcaag aagataaact tagtggagct agttcagatt atattcgttg
    30181 ggctaaagaa gttgatggag ttggatatgc ttatgtagtt tcagaatggg ctggagcagg
    30241 gacagtaaaa gtattaatac tagataaaaa cagaaaagca gcaacacaag aattaataga
    30301 taaggtccaa gaatatatat atccattgaa tatatcagaa ggagaaaata gagatgggaa
    30361 agctcctatc ggtgcattag ttacagttgt gacacctgac acattactta ttaatgtaaa
    30421 agctagtttt atatttagta atggctttag tgaagaaact gtattaaaca atctaaaaac
    30481 taagatagat aaatatttag ataagattga tttagggggg acagtctcat acaatgctat
    30541 acaggcgata gtaggctcta tgatgctgac agatgaaggt atagaagact tttctaatct
    30601 tactataaat gatgtaaaag aaaatataaa attgcaagac caagtggtcg gaatagggga
    30661 aatagttaac gaggtggttg gatgatagct tctaaaaaag gtaaagaaat gcttcttaca
    30721 ttatctccta tttatgaaca atctatcata atgcaaagct tatatgaagc tataggaagc
    30781 gaatttgata atctagagtt attggatgaa gaaatagaat tacaattatt ccctcagagt
    30841 gcaacatggg gtttgggctt ttgggaaaat agagtagggt tgattactaa cttagatgaa
    30901 gatatggaaa ctagaagaag aaaagtaatt gctaaacttc aaagtaaata tatcatgacg
    30961 cctaaaagaa tgtctatgat actccaatct tatacaggtg caaacataaa aataaatgaa
    31021 aatatatctc catatacttt tggtgttgaa ttaaccagta cccaaggttt tcctaaagat
    31081 ttagaagatt tatataagag agtaaatgtt ataaaacctt ctcatttagc tgtaagttat
    31141 aagttagttt ctttattgaa aagtaaaacc tattttgcac aaacagcaat tatgagcgaa
    31201 gaaataacta tatatccgta cacaagcaaa gaagtaaaag caagtgttaa agccaagttt
    31261 gcactagctc ataacatgag ttcagaaaca ttaacagtgt atccaagata ggaggtggta
    31321 taaatggctg atgaacaatt ttacacgata ctaacaaata taggtaaagc taagattgct
    31381 aatgcaggaa tgttaggtaa gccagtagtt ttagagaaga ttcaagcagg agatggtgga
    31441 ggaaattatt ataatccaac agaagaacaa acagcattaa aaaataaagt ttgggaaggg
    31501 aatataaatg cttttgataa tgatgaaaat aatcctaact ggattattgc aacagcatgt
    31561 atccctggtt cgataggcgg atttacagtt agagaaatgg gtcttataga taatgaagga
    31621 gatatgattg caatttgcaa aagccccgaa acttacaagc caaaaattga taatggagct
    31681 atgaaagatt tgtatttgaa atttatcata gaagtatcta atgtagagaa agtgacatta
    31741 gttgttgacc ctacagctat ttttctaact aaaaaagatg aagaaaaaat attaacaaat
    31801 attaataaac tagacactaa aatagataca accaaaatag aattaacaag caacatagaa
    31861 actgctaaaa cagagttaaa tactagaatt gacacagaaa atgagaaaca aaatattaaa
    31921 attgaccaat taatcgcagg tggttcaaat gtggcatcta cccaaacgat aacaattgat
    31981 gattgggtgg aggatgcaga aagtggattc aaagcaactg taacacatgg tttgttaaca
    32041 cagagaatag ttgtaaatat tatagatgct actacaaaag aaaatgtagt tacaaacttt
    32101 aaaattatag atgataattc tatagaaatt agaagtgagg taaaagttga attaaatgtc
    32161 tatgtgataa atggaaatgc agaaactcat tttattaatg caactgtgga tgataacaga
    32221 gtgtctgaaa tgactactta ttcgtctaag aaaatagagg attctattag ccgtatacag
    32281 cttatagata ccagtataag tattacagat gctaataata gatttacaag taataagtta
    32341 gatggggtat tagaagaaat aatggtagaa ataagtggcc aaaggactaa aggaattact
    32401 atagtaaata atttaataga tatgatataa gcgaggtgaa aatatgacaa caaagttaac
    32461 tgataatgct agtttagagg aacttatgac tacattagaa aatgtacaag ctgattttca
    32521 aactggtaaa aataatatat ctagtgcatt gggtagtcct tttattggaa cagataaatt
    32581 tggtacaact aaaacaaaaa tagaaacatt aaaaaatgta ttagtggaga caattaattc
    32641 taaaaatgtt tcagcaacat catctgaaac atttactaat ttgattgaaa aagttaactg
    32701 ggtttttcaa tcaatagaaa ttttttcttt aaaaaataga attcaggcta caattgtaaa
    32761 cactcctagc atcgtttaca atgaagtatc tagtataaaa ggtacattaa gattcacagg
    32821 cgaacttcaa tcgtctaaaa tgcgtgctga ttatgcaaca gcaaaaatag agatactatg
    32881 tggaaaccga aaagaatact tttatgttac tgacaatact cctggtgcat cttcttctct
    32941 tgtaagattt acaaaagata ttattgttga gaagggcatg gatataaaag tacaaatatt
    33001 attaacatct gtaggagcag gaaatttaga tggttcatat gcagctcgtg cagaaataga
    33061 agaattaaaa atattaaggt aggaataaat tatgaaaaaa caagtatatt ataattcttt
    33121 agatgaaaaa gaaaaaataa ttgatgaaaa ctctaattta tatattatag aaatatatga
    33181 aactttaaat gaaaattatt tagtattatc aagtagtcca gcagaagaca aagaacttag
    33241 ttatgaagaa ttagaaaatg aattattaat tataactaat gaattacagg gaggattgtt
    33301 gtaatgaata taaacaatgt tgtagtaaga atattagcag agagaatatt aaataaaggg
    33361 ttaaatcctt taaaaaatcg agaatttgag ttagatgatg taactaacac agactatagg
    33421 acagcagtag aggattatat aattaaacag agtggagtag tagaaggagc agaaccaaca
    33481 aagtaggttc tttttttata taaattaaaa ataatttcct tttttagtta tatatttttt
    33541 attcttatct ataataaaaa aattctatta aaaggagatt gaaaaatgaa gaaaataatt
    33601 gtaatttgca ttctagttct aatgttaatt atacctacta tgggggagat atttataatg
    33661 gtagaaaata taaagctaga ggggctagaa caaaacccaa caagttgtta catttctaat
    33721 ccagggatta tgggagaagc aaaatgtgat gcagcatact ttattatagc tgcagatttc
    33781 gttaacaatt taaaggtaaa ataataaatt aatacattta aaggacttag ataattctag
    33841 gtccttttta atacaaaaat taggaggaaa tatgaattta acaatagttt ttttagcaac
    33901 aaatatattt ataaaattag taatattagc aatagcattt gatacactgt taggttgctt
    33961 aagagcaata aaaacacatc agtttaactc aagttttgga ataaatggtg gaataagaaa
    34021 agtcgcaatg atagcatgta tattttttct agcagtagtt gacattctta caaagtttaa
    34081 ctttttattt atgttaccac aagattgggt tgatttctta cgactaaatc atcttggaat
    34141 atctgaattt ttctctattt tatttattct atatgaaagt gtaagtatat taaaaaacat
    34201 gtatttgtgt gggttaccag tgcctaaaaa attaaaagat agaataggta acttattaga
    34261 tactatgaca gatgaattaa atgttaaagg aggtagtaaa taatgaaaat atgtataaca
    34321 gtaggacaca gtattttaaa aagcggagca tgtacttctg ctgatggagt agttaacgag
    34381 tatcaataca ataaatctct tgccccagtg ttagcagata gatttaaaaa agaaggtcat
    34441 aaggcagatg taataatatg cccagaaaag cagttcaaaa ctaaggacga ggaaaagact
    34501 tataaaatac ctagagttaa tagtggagga tatgacttac ttatagagtt gcatttaaat
    34561 tcaagtggtg taggagcttt tggaacagaa ttattttact atagtgaaaa agggaaggaa
    34621 tatgcgcaga gggtggtaga taaactgtct aaacctttca taagaaaaaa aggagataaa
    34681 gaagtaggta atagaggtgc taaattagat aaaagtttat atattttaaa tagttctaaa
    34741 cctactgcaa tactaattga aagtttcttc tgtgataata aagaagatta tgagaaagct
    34801 aagaaactag gtcatgaagg tattgctaag ttaattgtag aaggtgtatt aaataaaagt
    34861 ataaataatg agggagttaa acagatgtac aaacatacta tagtatacag tggcgatgat
    34921 aaagtatcag cagacatttt aggattatat tataaaagag aaaaagaaag ttacttagta
    34981 acagatataa aagactataa accacataga acacaaaatc tatatgtaat cggtggagta
    35041 acttgtaata aaatgaagga aatgagtaag actacaggag aaaaatttac tcaactatat
    35101 ggtaatgatg tatggtcaac aatggataaa gctatagaat ttgtaaaaga taaattatag
    35161 aattgatata cactaaaata ggtaatacga attttatata agattcttct tacctatttt
    35221 aatgggttaa attttatact aaatcacact cgcacaacaa aatcattatt ctatgaaggc
    35281 aagattctac aatatcttct ccatattttt taatgaaatc atcaagctga gtctctatga
    35341 atgaacaata ttcttttaaa ttagcaatat atgcagtcat tatatgaata cattcatcaa
    35401 tcatatcatt aaacacaaac catcacccct agaaaacgaa aaataaaaaa tattcagtaa
    35461 attatgcata ttatattatc taaaatctta tctagataat acttttttaa aaaatatttt
    35521 acaagtagat atgcgaaact aggcattata actgcaaaac tagttatatt tattaatttt
    35581 atagccaaag agaagtacac agcaataaag ggaaatgata gatttgatac tctttacaaa
    35641 gcattagatt ttattgatag atagattaga aggtagcaac tagttagttg ttaccttctt
    35701 ttttaattat tagtttttac agagttatat acttctctct aattagataa taagatatta
    35761 ttgttacaat tgatactaat attactgata ttattgcagt atcaagtctt cttattaata
    35821 aacccattat tatatattct aaacctatta aatagtcata aaatatttct agtttagtag
    35881 cttttactct atttatattt ccatacttta attctaattt atctaaatat tttttcctgc
    35941 aataattagt attacgttta attaaatatc ttattgtagt tcctatagta aatgttattc
    36001 cataacttaa aaatataatg ctccaagaat tcataataga tacctcctct ataatataat
    36061 cgccagtaca tattagatat acttttatta atcaaagtat atcagcaaca caagtataaa
    36121 gagtatctaa aatattacaa atattaacag gatacataaa actatagtat aacctgtaat
    36181 aataaaacga acgaaaatta tcatactaaa acataagaca tgttataatt gtatcagata
    36241 aatatttgaa tatacactaa aagtactctt tttataagag tgcttatttt ttgaaattca
    36301 tcaacatata aacatcaaga accttactca acacacctta aaatcgattt aaatagttct
    36361 ttttgacaac ataatttata taatataata aaaaatagaa gtgtactgcc aatacacttc
    36421 tatagttgta actaagcatt ctcttgaggg ggagtgcttt tttcatttct ccaaacaata
    36481 ctatttcctt catcaataat tatcaagtca atttcaatat ctttcaaaat ctctgttgca
    36541 gtacaaatga gattatctaa gtcgaaactt cgagttatct ctagttgttt gttatttact
    36601 tctcttttta gtatataagt tttaaaacac atttgtatca cctcttaagc actaagacat
    36661 tcttgtaagc attcttctgc ttcttctaat gtagaaaatt catctacata ttggtcgaag
    36721 tttctaatat attcagtctt atatttagtg ctattcattt ctataaaagc aatagtttta
    36781 ccattgtcat agtagcgact tatcacgaag tatttttcta tggtagatat aatttcttta
    36841 tcctccagga ccatgttttc agcactgaaa ttaggtgaca tactataatt tccaatcctt
    36901 atgtattcac aattattatc atcattaaaa cggattttag cacgtctaac agtatcatct
    36961 tctttataag ttatagtttt atcagttctt ttaataacct ctatagtgta tatgcagtca
    37021 tggtcacaaa cactttttgc aaaataagtt ttacctactt caaattttat cattttaaat
    37081 acctccaata attaatttgt taagtttatt atacactata aagtgtcgtt tgtaaatata
    37141 aaaatgaaat taaatagttt tctttgtaaa ctaaatatag ataaaagaaa ctctatagtg
    37201 tataattata tatagaaaag tgaggtgttt atgatgtcaa tttcatttgg agaaaagata
    37261 aaaataatat taaaaagaaa aaatatgact ataggtgagt tagcagaaaa aacaggacaa
    37321 acaagacaaa atctttcaaa taaattttca agagataatt tttcagaaaa agaaattaga
    37381 gaatttgcag aaatacttga ttgtgaattt gatttttatt ttattataaa agatacaaat
    37441 gaaaaagtat aaataggggg aatttgttgt atggaagtaa ctttgcatac aggagagatt
    37501 aattttttta aagaccatga ggttgcaaag aattatatag gagatttata caattttata
    37561 gaggaatata agttctcaat ggacacagta tctattttga ctaatataga tataaagaag
    37621 ttaaatgact tttatatagg aaaaggagct ctacactatg atgaattagt acttataaat
    37681 agtgttgtta taatgccttt tatagatgta tttaaaaccg ctaaacataa ctatggaatg
    37741 ataattaaaa gcagtaaaaa agatgaagaa tagatatttg ataattgtaa aattatgtta
    37801 taattaaaat gcaagaataa tcttgcaaga aactacaatc taagggcgaa agttttcaga
    37861 aatcaccttc gtatgaaagg gggtgagtat atgaaaacac ttatgtttat acttgttata
    37921 gcgtattata ttactattgt gataaaatct attacagcaa tagtaaagtc tataataaga
    37981 ttactagatt ctataaatag attaaaagaa tctttacata aactaagaaa tcgtcctgac
    38041 tgcaatcaag acgatttcaa tgaataaatc attacttaaa ttattttgaa tttgaaactt
    38101 cactctaggc taatagattg tagtttcttt tttgtttttg atacgaattt acatctttat
    38161 tatatcgcat tttgagaaaa aataaaacta tgaatattag aaatatattt tattttatct
    38221 ctaagtatat atattattac ccacctaaaa aactttcaca aaaacaaaaa gagtatatgt
    38281 ttttgataac tggatgatat aacttataac tcattttaca attcttagtt catatatatt
    38341 tacgccacta aatttctcta gttttgccga tttagtatta ttctacaact aaaataatac
    38401 aatagaacta cttattattc aacaagattg tttgaaagtt aaataaattt atagaaatga
    38461 ataatatttt acgcttgaaa aaacagacta aatacgtaga atatttggta aaacttacct
    38521 gtattaattg caattagaag gttctattat taatttaaat taaaattaat tcaaggaagt
    38581 gacataaatg gaacaacaag atgtaataga caaactacgt aggggcgaat taatgctaat
    38641 ggagtatttg tggaataaaa agtctatttt atcaaaaaaa gaaataatag aagcaatgaa
    38701 aaagagatat aagtggagga aaagtactac agaaattctt ttaaaaagat tggtaaagat
    38761 gaaaacatta aaaaagaaga ggataggttt tcagtttagc tatgaagtat tagtaactaa
    38821 aaaagaatat ttaaatgcta gaaaagaaga aagagatata agtaaatatg ataatttttt
    38881 tgcacgaata ttcacaacta tacataaaag agataagatg acagaagaac aactaaaggc
    38941 atttataaaa aatatggaaa aaattggaaa aat
//
LOCUS       DAMJWS010000030        38586 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMJWS010000030 DAMJWS010000000
VERSION     DAMJWS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.030"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(68..679)
                     /locus_tag="PC399_001657"
     CDS             complement(68..679)
                     /locus_tag="PC399_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2592519.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(771..1760)
                     /locus_tag="PC399_001658"
     CDS             complement(771..1760)
                     /locus_tag="PC399_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF2592520.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYIEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAINNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(1827..2690)
                     /locus_tag="PC399_001659"
     CDS             complement(1827..2690)
                     /locus_tag="PC399_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF2592521.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(2796..3392)
                     /locus_tag="PC399_001660"
     CDS             complement(2796..3392)
                     /locus_tag="PC399_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF2592522.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(3432..4235)
                     /locus_tag="PC399_001661"
     CDS             complement(3432..4235)
                     /locus_tag="PC399_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF2592523.1"
                     /translation="MIKLTDEEFIVLVNYVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(4271..4711)
                     /locus_tag="PC399_001662"
     CDS             complement(4271..4711)
                     /locus_tag="PC399_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF2592524.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(4724..6826)
                     /locus_tag="PC399_001663"
     CDS             complement(4724..6826)
                     /locus_tag="PC399_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF2592525.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(7087..8790)
                     /locus_tag="PC399_001664"
     CDS             complement(7087..8790)
                     /locus_tag="PC399_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF2592526.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(8816..9775)
                     /locus_tag="PC399_001665"
     CDS             complement(8816..9775)
                     /locus_tag="PC399_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2592527.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(9911..10429)
                     /locus_tag="PC399_001666"
     CDS             complement(9911..10429)
                     /locus_tag="PC399_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF2592528.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFRVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(10472..10957)
                     /locus_tag="PC399_001667"
     CDS             complement(10472..10957)
                     /locus_tag="PC399_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF2592529.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(10954..11571)
                     /locus_tag="PC399_001668"
     CDS             complement(10954..11571)
                     /locus_tag="PC399_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF2592530.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(11636..11998)
                     /locus_tag="PC399_001669"
     CDS             complement(11636..11998)
                     /locus_tag="PC399_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2592531.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            complement(12742..13239)
                     /locus_tag="PC399_001670"
     CDS             complement(12742..13239)
                     /locus_tag="PC399_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2592532.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            complement(13414..14325)
                     /locus_tag="PC399_001671"
     CDS             complement(13414..14325)
                     /locus_tag="PC399_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2592533.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            15250..16101
                     /locus_tag="PC399_001672"
     CDS             15250..16101
                     /locus_tag="PC399_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2592534.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            16105..16575
                     /locus_tag="PC399_001673"
     CDS             16105..16575
                     /locus_tag="PC399_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592535.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            16588..17898
                     /locus_tag="PC399_001674"
     CDS             16588..17898
                     /locus_tag="PC399_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2592536.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            18110..18856
                     /locus_tag="PC399_001675"
     CDS             18110..18856
                     /locus_tag="PC399_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2592537.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            19218..20759
                     /locus_tag="PC399_001676"
     CDS             19218..20759
                     /locus_tag="PC399_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2592538.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            20794..21900
                     /locus_tag="PC399_001677"
     CDS             20794..21900
                     /locus_tag="PC399_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2592539.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(22231..23517)
                     /locus_tag="PC399_001678"
     CDS             complement(22231..23517)
                     /locus_tag="PC399_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2592540.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(23640..26738)
                     /locus_tag="PC399_001679"
     CDS             complement(23640..26738)
                     /locus_tag="PC399_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2592541.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSSDFEKLLY
                     QK"
     gene            27463..27615
                     /locus_tag="PC399_001680"
     CDS             27463..27615
                     /locus_tag="PC399_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592542.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(27908..28612)
                     /locus_tag="PC399_001681"
     CDS             complement(27908..28612)
                     /locus_tag="PC399_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2592543.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(28603..30210)
                     /locus_tag="PC399_001682"
     CDS             complement(28603..30210)
                     /locus_tag="PC399_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF2592544.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(30464..31642)
                     /locus_tag="PC399_001683"
     CDS             complement(30464..31642)
                     /locus_tag="PC399_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592545.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(31868..32362)
                     /locus_tag="PC399_001684"
     CDS             complement(31868..32362)
                     /locus_tag="PC399_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2592546.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(32363..33124)
                     /locus_tag="PC399_001685"
     CDS             complement(32363..33124)
                     /locus_tag="PC399_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2592547.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            33846..36680
                     /locus_tag="PC399_001686"
     CDS             33846..36680
                     /locus_tag="PC399_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2592548.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            36723..37967
                     /locus_tag="PC399_001687"
     CDS             36723..37967
                     /locus_tag="PC399_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2592549.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVVKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT        12983 a         6422 c         4486 g        14695 t
ORIGIN      
        1 taaaataaac tatcttccaa acattttatt atatgcttaa aagatagttt tgtgtcttta
       61 aaataaatta aattgtatat attccttcac ataacttttg tatattacta ggtggttgcc
      121 atttaccatc ttttttagta tatgtaatca tagttgtaac ttccttagtt ggtttcttat
      181 tgtaaaggtc tataatcatt tttgtcatat catcttgaac ttctttttct tttttatcgt
      241 aattcttttc atcagttaca taagatttta ctaaagtgtt cgcttcactc attactttac
      301 tagtatctaa agctttactt acaactttca aagttatctt atccttacta gaagattcca
      361 ctgttactgt cttttcaaca ccttgaatta atttaacaat tgtttcgtat atcttttcag
      421 cattttcttt accataaaga ggtgtcaatt gagcaatcat tgaaaccttg tccttgctgt
      481 tataagtttg aatctgttta tattcagctt ctgttaatgc atcagaaatt cctttttcat
      541 catacttata catttctaat gcagctaaat tagcttcaaa attcgacttt actttttctt
      601 ctgcattaga acaccctata gttagaacta aaactagagc cataatcgta gataatatta
      661 acttttttac cattttcata caaacatcct ccctcaatta aaataataaa aaatttattt
      721 atcaacatta taacataaat ttgtctaact atagaattat tttgaatatt ttaccacttc
      781 cagtctattc caataccgtt ttcttttgct ttatcgtata ttctttttgc tgttacgaca
      841 tcttgtacac ctatcccaac agttttaaat acaattatct catcatcatc ttctcttcca
      901 ggaatagttc cgtttataac attccctaaa tcaccactaa aatcatcttt acttatctta
      961 ccttctttta atggaattaa tatatctcct gactcagata aaactgcatc tgttgattca
     1021 aaaaatatct tagacgctct agttaaacag tttgggcaca actcttgcat atctggcata
     1081 tatgaaccta ctgcacttat taaagctcct tttttcacta agtttccatt aagtactggt
     1141 tgttttgatg gagtagctaa aactatcaca tctgcgttat ttatggcttc atcagatgat
     1201 tcaactgcaa ctattttagt attatattta cttaatttta agttcatttc ttctgcaaat
     1261 ttttctctac tttccttgtt tctactaaaa actcttacct catccaaatt tctagcttct
     1321 atcatggctt caagttgtgg ctcaccttgt cctcctagac ctatcaaagc tccaatctta
     1381 gaatcttttt tagccaatac gtctatagct gctccagagg ctgctccagt acgaatctga
     1441 gttacataag tgccatccaa tactgcacta acttctccag tttctccatc taataatagt
     1501 actgttgctg gaattactgg tttgcctttt tgagcatttt taggaaatac agatacaatt
     1561 tttattcccc cacagcctaa ttcttctatg tatcctggca taaataacat actagcatct
     1621 tgacttggaa cagatatatt ggttctcaaa ggatttacac tctttccttc acaataaatt
     1681 ctaaaagctt ccttatcagc ttcaattgca tctctcattg taaatacttt ttttatgtcg
     1741 tcttttttta aaagtagcat ttttataccc ccaattttaa taagtctcta tgctaaatat
     1801 tatagcataa catttaattt aatcttctat ttcaaatcaa ataaatctac atactctggt
     1861 tttagcatag aacttatcaa cgctttattt attgaaaatt ctggtattcc tgctgcatat
     1921 ggagttatat catataaatc aaaatataca actagattct catcttgcaa ataaaagttt
     1981 tgattctctt ttataccttt aaaattatat attccgatat tttcttcatc ctttttttca
     2041 agttcagcta tttgtctttt aatttcttca tctataactt ctttatattt agtattatcc
     2101 ttaaacaagt ccattaattc taaaaatttt ccagttctca tatccacatt atatgctata
     2161 tcttgataaa atccatgtgc tccaccacta taagtataat atctcacttt tatactaaga
     2221 gcagaatcag tattcttttt aagttcaaag tctacatttg caacaaactt atttttctcg
     2281 tcaggattat ccttaagata ctgctttgct tctttatatg atttattata aaattcaaat
     2341 atgtcatttt ttatcttatc atttgtagac ctttcaacaa ttttatttga atttgttatt
     2401 acaggcatgt ttatagtact ttctaaatac tcatcatttt tcactattgt tttacttcct
     2461 attgtactta ctgataaagt ttgtttacta tttgtattac cctttggtaa tgactggtta
     2521 ctcataacag tcactgcttc cttattacta tttaaattaa ttgaagcatc tttccttaaa
     2581 ttattatatt tatagtcatg tttaattgcc aaaacagcca ttatagtcaa tgttaaaaca
     2641 aatacatatc ttaaaacttt agcttttgta tcgactttaa aaaatttcat attatcattt
     2701 tcctcctaaa tataaatctc tcttattata tttggagttt ctagggtttt ttatccatat
     2761 aatttaataa aagattaatt tttatttata aaaaattaat ctttttgttt taaaatatta
     2821 atatgtctta tcaaacagtt tgaaatttca ccaagaggta attgtaacat aacagaacct
     2881 ctatcaaaag ctaccattgg cattccatat actatacaag aattttcatc ctgtccaatt
     2941 gtaaatgaac cattttcttt catttttaaa agtccatttg ctccatcata acccatacct
     3001 gtaagtatta ttcctattga atttttacct gctacttctg ctactgaatc aaacaaaacg
     3061 tctactgatg gacagtgacc acttattctt tctccctttt cacaagttac ataatatcct
     3121 tgtttatctg tacacaattt catctggtaa cccccaggtg caattaacac atttccttgt
     3181 tcgactctat cattattctt agcttctctt acattcataa tacactgctt gtcaagccgt
     3241 tgtgcataca tataagtaaa tatagctggc atatgttgaa ctacaacaat ccctggtgtt
     3301 gattttggta ggtcttttat aatttcaagt attgcttctg taccacctac agatgcgcct
     3361 atagcaataa ccatattatt aaaacgttcc acattaccgc ctccaaaaaa tcatatatca
     3421 aaaatacctt tttatcgttt atatatagca ggttttacat atttgtattt agtactactt
     3481 ctgttgatta cttctgcatg tcctacaaac aaaaaactat cttttttggt agcattataa
     3541 aatttattta taagttcttc tcttgttttt aaatcaaaat aaatcataac atttctacaa
     3601 aaaatcaaat caaaatgttg atatgagaaa tcattcatta aattaaacgg cttaaaaatt
     3661 acactttttc taatcttatc acaaatttta aatgtaccat ccttgttgtc tataaaatac
     3721 ttcttcttcc ataactcagg aacattgtta acatttgatt ctatgtatat acctctttta
     3781 gctttttcaa gtgagttagt tgaaatatct gtggctatga ttttaatgtt ccacaactct
     3841 ttattatatc caaaataatc atccaaagtc atagctattg aatacgcttc ttctccacta
     3901 gaacatcctg cactccatat atttatgttt cttattttat tattttcttc ccaaaaagga
     3961 agtatactat tctgtataaa ctcaaaatgt tgaggttctc tcataaaaaa tgtgtggtta
     4021 gtactcaatc tatttattag gtttatagct tcatttcctg tcttatcttt aaataataga
     4081 ttcatatatt gactaaaaga actaagtttt ttctctatca ttgtattgta taagcgacct
     4141 tcaattaacg ctcttttttt acttaaatca ataccatatt ctttttttac atagtttaca
     4201 agaactataa attcttcatc tgtgagtttt atcatgcaaa aacttcctcc tgctaattta
     4261 tcagaactaa ctatatgcta ttaatcaaaa actttcgaat atctaaaatt aatatagaat
     4321 tatcttcaac tttcgcaatc tcttttatat ctttattaat attagtgttg ttagaattag
     4381 tcgtctctag taaatttaga ttttctatat gaataacatc aacaacttta tctacaataa
     4441 ttccaacttg atactcatca acctttgtaa ttattataca agttctctca ttataatcag
     4501 ctttaggaag attgtacttt atatgcatat taataactgg tattattctt cctctcaaat
     4561 taataactcc acttatatat gttggaagac atggtacata cgttattgat tgaaatctta
     4621 tggtttcaat cacattttct aattctattc cataaataac atcattgaca taaaatgtta
     4681 atacttgttt aataatttca tctttagcca tactttaaat cccctaatat atttctataa
     4741 tgctatctgc atctataatt aaacttatac ttccatcccc aagtattgta catcccgata
     4801 aacccttatt ttttatatta aatcggttaa aataaactgg aaatggtttc acaacaactt
     4861 gctgattacc tattatttca tcaacaaaaa tgcatactgc tttatgctca ttttcaataa
     4921 ttatcatatt accattcatc agattggtta cttttgtttg aatattatat attttatgta
     4981 accttataat gggaatacat gctccatata ttgtaattaa ctctgtctta tttccttctg
     5041 taattatttg atttaagtct gtaagtttaa atgttcttct tatggatgat attggtaaag
     5101 taaatagtga atctccaact ttaaacttca taccctctac tatagaaaga gttaatggta
     5161 tttttatagt aaaggttgtc ccttcatctt gtttactttt tacttcaatt gaacctccaa
     5221 ctttttcaat acttttacga acaatgtcca tgcctactcc tcttccagaa aattcagtta
     5281 tagattcttt tgtagaaaag cctggagtca taatcaaaga attgacctcg tcatcagtat
     5341 actcattact ttgatgttta agaagacctt gttccttagc ttttgccaaa atcttatctc
     5401 tcataatgcc tctaccatca tctgaaatac ttatcacagc ttctccgcct agattttcag
     5461 cagataaaac tatctttccc acttcttctt tacctttcgc tatacgctcc tcaataggct
     5521 ctattgcatg gtccatagca tttcttataa ggtgcataaa aggttctgct aaattatcaa
     5581 ttacactctt atctacctct gtctctgctc ctatagttat taactctaca tttttatcta
     5641 attttttatt catatctcgt acaattctat gcattttttg aaatacacca gcaatttgaa
     5701 tcatacgcat agacattact atatcttgca attcatctgt aagcttgtgt aattgtcttg
     5761 ttgccttatt gaaattatct atatcaacac ctgtagaatt tacgctattt acaaccattg
     5821 attccataat tacaatttct ccaactatat tttgaagtaa gtccaatttt tctaaattta
     5881 cactcataat actctgctta cttattggta aatgttcatt ttcttcactt agactatctt
     5941 tttccatatc agttttttca tttaaaacat cttcttgtat ctcatcttct gtatagttat
     6001 tttgataact tttacttact ttattattat cttttaattt attttctact gcatcattgt
     6061 ttgatgtatt tttcatttct atttcttttt tactaatatt tatgtattct tttacataga
     6121 gattatcttc tatagtttta taaactattg ccttatcctc tttagctaca aattgtatat
     6181 aaaacccata acttttaata tactctgtag actctgcatt tgtttctaca tcttttggat
     6241 aaaaatctat aaattcacag taatctctaa gttcaaatac aagcatgaaa gctctcacat
     6301 tttccatcat gcaaccttgt tcaaagatta tttttactac atcagagtct tctttgtatt
     6361 caaaatttga atcgattact tctttttcgt ctttactttc taaatttgca ttatcctttg
     6421 atttcataga acttgataat tccaaagtac tcacatactc ttcaattcct tcaataaaat
     6481 tagagtaatc agaagatata caacctcttg attgtaactc atctatatct cttcttataa
     6541 aatctaatga ttgaaagact aaatcaaata ataggtcatt gtctaaaact tctattttac
     6601 cttcacgtat agcataaaac aagtcttcta cactatgtgc taaagcagat atattgtcta
     6661 accccatcat agatgctgaa ccttttatcg tatgcattat tctaaaagtt tcattaatgt
     6721 tgttatgagt aaatactttc tctttttcta tttgaatcat aatttcatct aattgttcga
     6781 gtaataaact tgtctcaaat ataaacatat ctaacatttg tctcatactc ggttcaatat
     6841 aattcacatt tatcacctac gacctacaaa taattttcta ttatatatgc atctcataat
     6901 ttaaatcctt gatgattctc ttatctcact atatgtacct cataatttaa atacacaatt
     6961 attctcttac ttcaatatat atacatctca taatttaaat acatgataat tctcttattt
     7021 caatatatat acatctcata atttaaatac atgataattc tcttacttca atattcacct
     7081 cataatttaa acccatgatg attctcttac ttcactttcc tttagatgaa atttttctac
     7141 taattctttt aaaatttgag cttgagcaga catctcttct gaggctgctg cactttcctc
     7201 tgctgttgct gagttcattt ggactactat ggaaatttgc tctattccag ataaaatctg
     7261 tgtaattgct acttcttgac tatgtacagc cgaagaaata ccattaatat aattagtagt
     7321 tttttctaca ccatttacta ccaagtccaa tgcatttgca gtctctttag caattctgac
     7381 accattttct actgttttaa tagcttcttc tatgagaata gctgtatttt gagctgcttt
     7441 tgcactttta ccagccaaag acctaacttc atctgctaca actgcaaatc ctctaccata
     7501 ttgacctgct cttgctgctt caacagctgc atttaatgca agaatattag tttggaatgc
     7561 tatctcatct attgttttga ttatttttga tatttcactc gatttttcac ttatttcacc
     7621 cattgatgtc atcatatcat ccatacatct acttccaaaa ccaacttgtg atgcagattg
     7681 gtcagatacc tcacatgcaa gactagcact atctgcacac ttcttaattc catctgatat
     7741 ctcattaatc gtggaagcta attcttctac tgaactagct tgttcagtag caccctgtga
     7801 aagagcttgt gcacctgctg caacttgttc tgaagcactt gcaacttgct ctgctgataa
     7861 atttatttca gaaaaatcac cattaaaaga gtttattata ctttctaaag atattttaat
     7921 tgatttaaaa tcacctataa attcttggtc tatctctact cttaaatctc cttttgcaat
     7981 actagttaat gtactgtcta taaggtctat gtaatttaaa agcatacttt gcatcttttt
     8041 gacagaactc actaatagcc ctatttcaga tttactttct tcaacgtcta tacttgttct
     8101 taagtcccca gatgcaattc ttgaaaagtg tttttctact ttattaatag gaatcactat
     8161 ttttaatttt attactgtat attctattat tgtcaatatt attaaaaggg aaagtataaa
     8221 tattgttctt aatattagac tttgtaattc tttttttacc tctacaattt tggaatttga
     8281 acgggtaact atatcctctt gaaattcatt tattaaagaa tccattttct cttgggagtt
     8341 tatatagtca ctaccaaaaa ttatactttg agctttactc ttatcattac tttcgactgc
     8401 ctttattgca ctttgttctg ttggtactaa agaatctgat atttcttttg cttctaagat
     8461 tttatttgac tcgctctttg taagaccaat tttttctaat ttgtttaaag ctgattctct
     8521 tgtttttccg tataattctt tttcataaat atctttattc tttttttctc ctgtaaatac
     8581 atatcttctt aatacatcac ttatctctct agttgtatct tttatatact gactttgagt
     8641 cctcaaatta ttcatatcat taatactttt ttctactttt tcaaatgctt tataagatga
     8701 aatagaagaa aaaatccctg ctagaacagc caatactata ataatcagca atacaattgc
     8761 taatttagaa agctttactt ctcttttcat atctgtactc cttcataaag taagtttata
     8821 tcttttttaa tcctttcatt gtactcacta cgctacaatc ttttcctaag tttttaccct
     8881 catacattgc tttatctgct aaggatatgt tttcttctaa tgatatattt gagtttactt
     8941 ctactactcc taatgtaata gtgcacttta ttttatgttc tttatagttg aaatcaaact
     9001 cctttatctc tttacggatt ctttctgaaa gtatcttagc accttctatt ggagtatttg
     9061 tgagcattat caaaaactct tctccacccc atcttgatac agtatcaatt tgtctacaat
     9121 tcttttttat tatacttgat attactgtta acacgaaatc ccctgcttca tgcccataag
     9181 tatcattgat aaccttaaag tcatcaatat caccaaggat aagggatatt gatgtttcac
     9241 tatatttatt aattcttctt tgctccataa gctgattaat tatatgtcgc ctattgtaaa
     9301 gacctgtaag ataatctctt acaacgagtc tattcaattt ttcaagagaa gattccagtt
     9361 cattatttat cttttttaat ttatcttgag atttctttac ttcaagatga gctataacac
     9421 gagcttttaa ttctgtcata ctaaaaggtt tcaccatata gtcaattgct cctacaccaa
     9481 atccttttac tatgtctgta tctgtatttt ttgaagttat aaaaattata ggtatatctg
     9541 ctgtacgagc attagatttt acttccttac aaaactcaaa accagagtta tctggtaaaa
     9601 ttatatcaag caaaataatg tctggtataa attcttcaat aaattctttt gcctctattg
     9661 cattaaatgc ttttttaacc ttaaaattat cttttgtaag tgttttatct attaagtcac
     9721 acatcagaac actatcatca acgactaaaa ttgttccctg ttgcttattt ttcatataca
     9781 aacctctctt aaacataata tattcaaatt ttaatgattt aaaattttta tacttaataa
     9841 ttataccact atattcttaa atgttctcat cacaatcttt ttataataac actccaacta
     9901 catccctatg ttacatgata gtgcaatcaa acccattgat taattcatct attaaaactt
     9961 catctttatc tgtcaaaagt ttctctaaat ctagtaaagt aattatttta ccttctaatt
    10021 ctccaactcc acttacaaaa taatccgaat attctatagt aactcttggt ggaggtgata
    10081 ttttactttt ttctaagctt actatatcat caactctatc aaccacaaat ccaactacca
    10141 agtcctttat ttcagttact actatataca tgcaatttgt agtcattaat tcatcttttt
    10201 taagtctcaa acgcatgtct attatcggaa tgatttttcc tcttagattt attatgcctt
    10261 tagaatatgt aggtagttct ggaataaata taatttcttg atttccagta atttctatta
    10321 ccttttcaag ttcaacccca aagtcttgct catctactct aaactttaga tacttaattc
    10381 cttcatcaat tagtccataa tctaaatatg ggtcatctgg aaatctcatt taatacctcc
    10441 ctcctaaaat acactttcta tgaaatatat attataattt cttcttacct ttatttattg
    10501 aatctacaag taattcaccc gtctcagtgt aaaaacgtat tgttctacca taattcatac
    10561 ctgtgtcctc agctaatatt ggaatattta atcctaaaag agtttttttt actgcctcaa
    10621 cattcctatg tcctatactt gcaattactg aattgctact tcctaaaaac atctgagcac
    10681 ctccagcaat ttttgctttt atatatattc tatttgcacc tatattctcc atagatttaa
    10741 tgagctcatt tattcctgtg tcagcaaatt tacacttatt ttcaatgtta atagcatctt
    10801 tactatatgg caacataacg tgtaacattc caccaattcc agctacttta tctatcaaac
    10861 atactcctac acaagaacca agtgcataag taactaaaac atttggtcta taggctatct
    10921 tcatatctgc aatgttcact actatctctc tattcatact tctaatccca acctcacgag
    10981 tatttctcta agtgagctca tttctggaat catcaaaatg tgactagtaa aataattatc
    11041 actcatttta aatttatttt ctataaataa gactttgtca ccaacatctg aaaatcttat
    11101 cataggtaca tttagaacag ctccaaccat atcaatacat atatctggaa ttgaaacact
    11161 tatagtcatg tttaacatag atgcaatagc atttacatat gaacttgcca taatatttcc
    11221 tatttcctta attgctgaaa tttcaattgc ctcaatctca tctaaaaaag cttcttcttt
    11281 attaaaaaga gaacttagca taagttgagt tattgattca tccaacaaaa acataaacat
    11341 tccattgata tcacctttca tatcgagtaa aatacatact actttgtttt ctggaccacc
    11401 aagcaaattt gtcgcttcat tatattcaag tatcttgacc ataggtatta atacttctac
    11461 attgctattt atcatggatg caagtgcagt aattgcattc ccagagccta tattaccaat
    11521 ttctctaaga gcatctatat gaacatcatc aagttctaaa taatttataa tataaacccc
    11581 tccctttatt attgcattta tttgcttttc attgtattag tgtatattta cacttttatt
    11641 ttaaagcatt atctacaact ttaactaatg tttctgtttt gaagggcttc actataaaat
    11701 ctaacgctcc aagttttatg gcttctacaa tcatattctc ttgtcctatt gcagagcaca
    11761 ttacaacctt agcacttgaa tccaactttt ttatttctct tagcacctct aaaccatctt
    11821 tttttggcat tgtaatatca agcaacatca aatcaggctt ttcatattta aatatattac
    11881 acgcttcttc tccatcattt gcttctatta aattattata gccactttta ctcagagctt
    11941 ctttaattat cattctcata aaaatagcat catctactat caaaatttta ttttccataa
    12001 aactaactcc tccatatgta caacaataaa cgcttttatc ttcagttatt gtctatacta
    12061 tctgactatt tttcgctaca caataaatac atttcattga taaataaaag taattattta
    12121 tgccatatta tacttaattc tcttaacata atttgtcaac aaaaaaactt atatatctat
    12181 tgtaatatat gtttggcgtt ttgacgagtt attaaaaata taattgtata aaacataaaa
    12241 agttatcaaa agtaattaaa ttataataaa acaactttat acctttttta tgtttgtata
    12301 aaaaaatttt tcagattcat taaaatcatt tctgtagatt catataggat ttgactttct
    12361 caatatctaa tagatagttg tttatttact taattatatt tctctatatg tattatcgat
    12421 taaattagat ttagctttat acattatttt ttatattttt caaattgtat actcataaat
    12481 ccagaagtat tctaatgtgt ttataatact ttttttcata ttttaaatta aataacaaat
    12541 cattatattt acaagtatca tttatcaata aacaatatta aatgaagaat atgtctgaag
    12601 attcaaagtg atactgataa tattaattac taaaaaagca gtaaatcttt tacagattta
    12661 ctgcttatag ttataaataa taaaactatc tataataaat gtactatact caatacttca
    12721 ttcaatatag taataacaaa tctactttat tcctatataa atatgaactt cttgtttctt
    12781 tgtatcaaaa gtattacatt gatactcttc aaaatcactt atataagtcc tatcaaaatc
    12841 catttgccaa aactcaaacc aaaaatctcc tacttctttt tgacctccta ctataacaaa
    12901 ctttgcatac ttacctgctg gtataacttt actaataata ctttctccaa cagtattatt
    12961 agtgtttaat tcttctaaat tcttctcttt atcagaatta ctatttactt cacaacatgc
    13021 aacaaagcta tacggacttg taaaatcacc ctggtaatct gtatatagac ctattgtctt
    13081 gtcattcttt ttacccttta tagcattata tatccctttg cccataaatt ttttccacaa
    13141 ttcacctata tcatttaccg cttgtccatc tttatttgta gtctccttcg atattcctat
    13201 aactattttc tcatttatct ctactatttc ataattcata ctttatacct cactttgttt
    13261 ttattaatat attttatttt gaactaattc aattataatt tataatatac gacaccttta
    13321 tgtcatattt ttaatataat taaactcagt gagatattat atactaaatg caagatatta
    13381 tatatttaat aaaatcttta atctagcatt atattataat gaataaattt tagacaactc
    13441 ccttagtcta gttattacat tatccctaac atctttaggt tcaagtactt caacattgtc
    13501 accaaatgat aaaatatatc cataaatcca ttcaccaata ggaaattctg taattacatc
    13561 atatgtatca tcttcatttt tagttattaa atccttatta aaatcatcaa atactctaaa
    13621 aagcatcttt tctttaaacc tcaatttcaa cgttatagta ttttccttaa ttactttaga
    13681 atcattcaaa catacttctt ctattatctt tcttgaaaat acttcatcct ttatagataa
    13741 attccttatc ctactaattc taaatatcct aaaatcatct tttagtttac aaaatccata
    13801 caagtaccaa gtcttactct tatacataag cttcagtggt tcgacagttc tgtttgtctg
    13861 gcttgacaaa ctatttacat aattaaaatt tatagtatta cgccttaaaa tagcttcttt
    13921 tatttttgta aatttatcat cttcattaaa attacttccc cattcagtaa agtctatttc
    13981 aatccagttt gaaaaattct gctctccaaa tattgagcca attttattta ctactgaatt
    14041 tatttctgga tacttagtcg cctgtaatgt tttcagtgcc actattaaac tctctttatc
    14101 tttttttgac aatatagcct tgtctataga atattcttct attaaagata tccctcctcc
    14161 tttgcctttc gtcatatata ctggtactcc cgacatagac agagtttcta tatctctata
    14221 tatggttctt gtagaaactt caaatctatc tgccaactct ttagctgtta ctaatttttt
    14281 atttaataga attactatta tttctgttaa tctatttatt ttcattcatt accacctact
    14341 aaaattatta aatttattta atctaaatta atcatactaa tttttaaata aataaaatct
    14401 tagaatctta tctaaattat tcttacatga ttttatttac tttctattat ctttctcact
    14461 ttggatatta tcatcttaat ttatttatta ctatatcaat ttacttatta tcatctcatt
    14521 tgttatcgtc ttacttaatt tacttatttc tatatcaatt cacttattat tacctcactt
    14581 gttatcatct tacttaattt acttatttct atatcaattt acttattatc atctcatttg
    14641 ttatcgtctt acttaattta cttattgcta tgtcaattca tttattatta tctcacttgt
    14701 tatcgtctta tttaatttac ttatttctat atcaattcac ttattatcat cttaattcat
    14761 ttgattatta tcatttactt tattcaattt ctatatcaaa aattagtatt tgtacttaat
    14821 acttaaaatt aatttaaagt ctttacatat tatttatttt ttattgtaat attaatttcc
    14881 atactttgct aagctacttc cactcattta ttttaaaatt tcaatttact atttctcaat
    14941 tctatattat tatttttcaa ttttatatta ctaattttaa agctttttag ctattattcc
    15001 tacaatattt aaacaatttt ttaataataa agctatatta caatatattc aaaatatgta
    15061 atattttaga tatttttcgt tgacaatgtt aaatttttgt tttatcctta attaaggaca
    15121 tgttattgac atttctgaaa cttatttgaa atagtttttt cattttattt aggaaaacgt
    15181 attataccta agaaaacgta ttttctcatt tcagataagt aaaatattta ttcttttggg
    15241 gaggaaatca tggaaattaa taaaaaacat tttattcaag tatttttaac ttgcttaatc
    15301 ttggtagtaa tatgtgaatt tatagggcaa agaactatag ctattggaaa agcctctata
    15361 gtactgttcc ctatgcttta tgcagttata attggactta taataacacc agatttatta
    15421 ggtaaaaaaa ttaaagcact taaaaaagtg gttggagaaa aagaaataaa tattgctggt
    15481 gaagttgttg gaattgcact agtaatgctt ggtataaaat atggtactac agttggacca
    15541 aacattgaca aaataattca agctggacca gcatttatcg ctcaagaatt tggtcatata
    15601 cttgcaccaa tcgttgcatt acctcttgca ttaatgtttg gaatgaaaag agaagctgtt
    15661 ggagctactg caagtataag tcgtgaacct tctttaggag ttatatcaga aaaatatggg
    15721 ataaactcac cagaaggtag tggtgtactt ggtacatatt taatgggaac agttcttggt
    15781 actatatact ttagtgtact aggttcaata tctatttata gtggtcttca cccatatgct
    15841 ttgggaatgg cctgtggtgt tggtagtgga agtatgatga cagcagctgc tggttcatta
    15901 tctaccatgg taccacctga aatggcagat acaatacttg cctatgctgc aacaagcaat
    15961 atgatgtcta gtataacagg aattatattc ttagtttttg tatccttacc atttactaat
    16021 tttatgtata agattttaga acctaaattt tcgagaaata aaaaaactaa agaaattaaa
    16081 tctacagaag gagagtgcta ataaatgttt gctaatttaa atttaaagaa aagttcatat
    16141 caattattgg ttatgtctgt tattggagtt ataattctta taggacaaag aattagcgtt
    16201 ggtacatcta tagttacagc tcttcctggt atggtgatgc taatattagc tgctatggca
    16261 gctatgatta taaaagattt atttcctaaa tcaatctttc cagcttttgg atttgcaact
    16321 ataatcggcc ttttacttag tatgccatat agtccaacat ctgaagtatt cttaactaac
    16381 actaataata ttaactttat ggctataact actccactac tagcttttgc aggaatttct
    16441 gttggaaaca aaatagaagc tttaaaagaa atgtcatgga aaatagttat aatttcactt
    16501 attgtattta caactatatt ctttgcatgt gcgagtatag ctcatatagt tttaagtatt
    16561 caaggtaaaa tataggagga atttattatg aataaagaac aattaaaatc tctagtaata
    16621 aaaactatag atgaaaatag agaaaaaata ttaaaaatag gtcaaggaat atacaaaaat
    16681 ccagaatatg gatataaaga atttaaaact acagaagctg tttcaaactt ttttgagcaa
    16741 gaattaaatt tatctgtaga aaaaaatata gcagttacag gatgcaaagc agatgcaaat
    16801 tctagcaaga aaggtcctca tatatctata cttggagaat tggatggtat atcatgtaaa
    16861 gaacataaag atgctaatga aataggtgct tctcatactt gtggacataa tattcaaata
    16921 gctggaatgt taggtgctgc tgttggttta gtaaaaagtg gcgttttaga acatttagat
    16981 ggtaaagtat cttttatggc tactcctgct gaagagttta ttgagttgga atacagagag
    17041 caattaaaaa aagaaggaaa aataacttat tttggtggaa aacaagaatt aataaaaaga
    17101 ggttattttg atgatataga catatctatg atgtttcatg cttctgattt aggagaagat
    17161 aaggctctag ttggtcctga gagtaatgga tttgttggaa aaaaggttaa atttataggt
    17221 aaagaatctc atgctggttc tgctccacat gatggaataa atgctttaaa tgcagcaatg
    17281 cttgctataa acaacgtgaa tgcacttaga gaaactttta aagagtctga aagagttaga
    17341 ttccacccta tcatcacaaa aggtggagat atagtaaatg ttgttcctgc tgatgttcat
    17401 atggaatcat atgttagagc tagaactata aatggtatga ttgatgctaa tgaaagaata
    17461 aataaggctt tgatggctgg tggaatggca gttggagctg atgttgagat aactgaaatt
    17521 cctggatatc ttccaatatt acgatacaaa agtcttgata atttatttaa aaacaatctt
    17581 gaagatttag gtctaaaagg aaaagttatt gatggtggag attttacagg ctcttttgat
    17641 tttggagatg tgtctcatat aatgcctact cttcacccta tgattggtgg tgtaaaagga
    17701 gctcttcata caagagattt tgaaatagtt gatgaagatt tagcttacat aattcctgct
    17761 aaagcaatgg cacttacagt tgtagacctt ctttttgata atgctaagga tgctaaagat
    17821 atattagata cctttactcc agttatgact aaagaagaat atctaacatt cttagaaaaa
    17881 cacgataaaa ctttataagt ctatttttat aatagggtgg tatctactgt aaaagttata
    17941 ccactctttg tttttgtaac agtatacttc ttttctttta gtttttagat taaactaaga
    18001 tatattatcc tccttaaaac ttttagagct acgttgaaat atcagtagaa ctatgctata
    18061 ttctttctaa ttaatataaa tttctattct aattcaaggt ggtgaaaaaa tgtttaatgc
    18121 aggaaatcga attataaata catggttgta ccctattcaa gatggctttg tcttgattga
    18181 tactggatat gaaaatggtt ttaaacattt caaaaaacga atttctaaat tcaacataaa
    18241 aatcaaagat attcgataca tatttctaac tcatgctcat gatgaccatg ttggttttct
    18301 aaatcagatt ctagacgaat ctcctaatac aaagattgtt atgcatgata aagctttaga
    18361 aattctatat aaaggtcaaa actcttttat tggtggttgt accagccgta ctgcctttct
    18421 cttttgccaa attatgaaac tatttggaaa aggtgagcat agtttttctc cacttaaaca
    18481 agaatttgaa aaaaggtgta ttcttgtgtc tgacaagaat cgtaaagaaa ttgaaaaaga
    18541 actaaatgga aaaattatag atacaccagg acatactgct gattcaattt cactactaat
    18601 tgatgaaggt gttttatttt gtggagattc tgcaatgaat ggatttccaa gtactcacaa
    18661 aattaccata tttgcagaaa gcaaactaga atttattcaa tcttggaaaa caatcatcaa
    18721 tacaaaacct aaaatgattt atcctggaca tggaaaacca tttgaataca gtaaattaga
    18781 acaaaatcta atctatgtaa ataaaataaa tttatatcct ctacataatt caaaagatat
    18841 tcacaggttt ttgtagaaaa tacaaagtaa atttatttga ttaattaaaa aatctaacac
    18901 tttactacgc tacttatttt aataacttaa tcttccacta gcttttcata aaattttgtt
    18961 ttaaaattaa ttttaaagcg catatgttat ttaaatacat tagataaaat tcgtcattaa
    19021 tctacttaaa gcatatttaa ctaacaccac tcaaccagaa aattcagaaa actgcattga
    19081 cacaggaaaa agtttatttt atactttttt taggacatat tgtggacacg tcttaaatcg
    19141 acaaacagaa tttaaattgt aagattaacc acaatactta ttttttaatt atatttttaa
    19201 acattaggga ggatattatg aatgataacg tagttaagaa gaaaaacttt tttgacagga
    19261 cattaaacag aatagaaact gttggtaata aactaccaga cccagttaca atatttttag
    19321 ggttatgtgt acttttactt atactatcta gtctagttgg ttctatgggt atatcagtag
    19381 ttcatcctgg tactggagaa actataaccg ctgtaaattt attgactgta gaacagcttc
    19441 aaattctttt aggaaacata gtcagcaatt tccaagggtt tgctcctttg ggtctagttc
    19501 tagtaactat gataggtgct ggtgtatgtg acaaaacagg acttatgaca gcaacaatca
    19561 aagcatcagt aagcaagatt cctgaaacaa gagtaactct tgttgtaatg actataggta
    19621 tgcttgccaa tatagcttca gatgcaggta caatattatt tccaccatta gctgcacttg
    19681 tatatcttgg tgttggtaga catccactta taggtttatt ttctggatat gcagcagtct
    19741 gtctaggatt tgccgcaaac attatgataa gtgtcaatga tatacttgct gcaagtttta
    19801 cagttccagc agctcaaatg attgattctg gatttcaagc caatgcaaca atgaacttat
    19861 tttttatgat agcatcaact tttgttctaa tacttttggc aacatgggta acagaaaaaa
    19921 ttgtagctcc tagatttggt aaatatgaag gaaatgcaga actagatgtt gatggaagtc
    19981 ttagtgatgt agaaaagaaa gggcttaaaa aagctggtat aagtatatta atatatgtgg
    20041 ctattatagt agctcttagt gtaatcggta aaaaaccatt cttagctgac ccagaaacag
    20101 gagatttact atcaaacaat gctcctctta tgaaaggtat ggtacctata atagcactag
    20161 catttttcat acctggtgtt gtatacggaa aaactatagg taaaataaag aaggataagg
    20221 atgtagtctc tataatggct agtgcaatga gtgatatggg tggatatata atactagcat
    20281 ttgctgcttc acaatttttg caattattca caaatagtaa tttaggtctg atactagctg
    20341 taaagggcgg agaattttta aaatcagcag gaataaatgg acctttattg ttgataggat
    20401 ttattatatt atcatgtttt ataaatttat ttataggtag tgcatctgca aagtgggcta
    20461 ttcttgcacc tatatttgta ccaatgttta tgatggttgg atacaatcca gcacttactc
    20521 agatggctta tcgtataggt gattgtataa caaatccact tagcccatta tttccgtact
    20581 tccctattat actagcattc acaagaaaat acgataaaga tgctggtatg ggtactgtaa
    20641 ttgctaatat gataccatac tctatatcat tcttaatagt gtggactata ctattaatat
    20701 tgtttatggt atttaatatt ccactaggac ctggaatact tccttcatat agcatgtaat
    20761 ttatttaaat ctttctaaaa ggagaaataa tttatgttag ataaaaatag acttataaat
    20821 aattttatgg atatggttag aattgatagc ccatctaatc aagaattaga aatgtctaag
    20881 tggttggtaa actacttaaa agaaagaaat atagacgcca ttatagatga tgcaggtgaa
    20941 aaatatggtg gtaatacagg taatgtcatt gcctatataa aaggtgaaga aggaagtaga
    21001 ccactttgtt tatgtgcaca catggaccaa gttcaacctt gtttaggtgt aaaaccaata
    21061 ctagatggta atgtagttag aagtgatgga actactacac ttggtgcaga tgataaagct
    21121 ggaatagctg ctatacttga agctttagaa catgttatta cagaaaaaat accacataga
    21181 gatatttatc tttgttttac aatctgtgag gaagccggta tgcatggtgt caaaaacttt
    21241 aatcctgaca acttgccttg taaagacatg gtgattttag attctggtgg agctattggt
    21301 tcgatagcat acaaagctcc tgctcaacaa tctataaaaa tctcttttca tggaaagaaa
    21361 gcacatgctg gtatagaacc tgaaaaaggg ttaaatgcta tattagtagc ctctcatgca
    21421 ataagcaaca tgcacatagg cagaatagat tcattgacta cttcaaatat aggtaaaata
    21481 gaaggtggtg gagctacaaa catagtaact gataaggtta cattgactgc tgaaattagg
    21541 tctcatatac ctgaaacttt agagtatgag cttaatcata tggaaaaatg ttgtaaggat
    21601 gctgcatcta aatttaatac tacttatact tttgaacata gtatgtctta ccctagtttt
    21661 gaacttagta gagatagtca tgttttcaag ttaagtgaag aagcaataag acaagttggt
    21721 gttgttccaa atccaatggt aattggtggt ggaagtgacg caaatatatt agctaatctt
    21781 ggatataatt gtgctatatt aagtctaggt atgtatgatg tacacacagt taatgaatat
    21841 gtaaatatag acgaattata tgatactgcc aaaatagttt atcatatgat taaattataa
    21901 atatcataat ataactcttt aaattataaa aataaaaacc tatgtatctt caactttata
    21961 agttatatat taaagttgtt ttacataggt tttagtattt tatataataa tttgttttaa
    22021 catttttcat aataagcttc aatattttgc ataataattt gttctagcat ttttaataac
    22081 aagctttagt attttatatt aataatttat tctagtattt tacataatca tttgttctaa
    22141 tttttttcat aataagcttt aatactttat atagtaattt gttgtagaat tttatacaat
    22201 ataatgattt atacagtttt ataaaatatc ttaaaacagt atttcagtaa tattttttgg
    22261 tcttcctcca ccaatactag tctcttttgc acatattcga cctaaacctg catttactat
    22321 tttatttaat atacgtcttg cacttctttc acttacattt agatactctg ctaactcttt
    22381 tgaatcataa atcttacttt ttctattctc atttatagca ataatttttg caactgactc
    22441 acaactcatt ccagtctttt tagatatttc gattaccttt tcatcagata gcatcaaaga
    22501 ataatttaat tcattttcgg aagctaaagg tcctcttatg ttttcatctt catctaccaa
    22561 atatatacca atttcttttg aatctatgga gtgtcttaat gctttatgtg cattattttc
    22621 agcctgatac gcagttactc cagtaccaat tcctatacta agagaaaagc ccatgtcttt
    22681 tatctctctc tttagattga atagcttatc ataattatac tcattatcag ctaccccttt
    22741 atgaacaaat acaacataat catttcttcc aagtaaaaat aaggaacctt gaatacttct
    22801 tacatagttt actacaagct tatccatatc tgattttttt atcatgtcag aataatagtt
    22861 atctatcttc tctttataat ctattaaact caatatttca acagcaattt gagaaaatct
    22921 tgccttactt agagcatatt cactctttac cttgtcataa ctttctttga ttaattgtat
    22981 agtagcttgt aaacgaaata caggatagcc tctttctttt aattcattat atactgcacc
    23041 aaaaccagtt aacattatat ccgtcttatt ttcttcaaat aattttatat gccattctac
    23101 aagttcagac tctcctactt ccaaagaaaa tggattacaa aatacactcg ttgctttaat
    23161 atcaaattct ttaagtgcat cttccacaac atatctatca actacatcta tactgaattt
    23221 atctaatttt atatttgcac tttttattgc ccaaaatgcc ttcattatac tcgtcccact
    23281 tctcggtaca aatgaatgtg gcttacttat ctcatactta gattttataa attcataaac
    23341 tgcacatcct gtaaatataa tagcatcaca ttcgtcttca catttagaaa taacttctat
    23401 tgtatctact actttttctc ttacatatat tttagtcttt aaatcactgt ctatctgttt
    23461 taaatcatcc tttatcttaa acccagagtc tgaaggacct ataataccca ctatcataaa
    23521 cttcgctcct aatccataaa tattatactt tttaaaaatt atatcctcac tattttctac
    23581 ttctatttaa ttattttgaa ctactccatc ctacctacat ttagaatgga ataattagct
    23641 tatttttgat aaagtaactt ctcaaaatca gaacttggaa taggcttaca ataataataa
    23701 ccttgaatta ccacacaatc taaactcact aaaaattcag cttgttcttt tgtctctaca
    23761 ccttctgcca ctacatccat atctaaatct tttgccatgg ctatagtgtg ctttaaaact
    23821 attttttctc tgtctgtgat tccttcctca ttaaagaaac cacaatctat ctttaatgta
    23881 tcaatattaa tattttttaa catatttaat gaagaatatg ctgaaccaaa gtcatccata
    23941 tttaaaacaa taccatccat tctaaggtcg gctattatct tagtaaataa ttttatattt
    24001 ttaataaata aactttctgt aagctctaat tctaaaaaag attttggtat actatattta
    24061 tttattaaac ttaaaacaat ttctttaaaa tgtggattat gcaaatgtaa tcttgataca
    24121 tttacagaga taggaactgg ctcttcacct aaatctatcc attttctaat caaagaacat
    24181 acttgttccc atacatacat atctaatctg ataataaacc cattcttctc aaatagtgaa
    24241 ataaatttaa ttggaggtat tatacctttt tctggatgaa cccatctaac caatgcttct
    24301 gcaccaacaa ttttatttgc attaagatta tacttaggtt gtaaatatac atggaattgc
    24361 ccattttcaa gtgcgtattc catttccctt tccatctgtt tttcttcaag tgcatcattt
    24421 cttatagtat catcataaaa tatatagttg ttgatacttt gtttctttac ttttttgtga
    24481 gcatatcctg ccatttcaca cgcatttgta attggaatat gattatccaa aattctataa
    24541 accccaaaac atggtactaa catatattca aatttttttc tatttaagct atttttaatc
    24601 ttataaatta ctttatgaat atctctatca gagtaactct cataaaaaat gcaaaataca
    24661 tctgcataca tacgacaaca tataccatta gaaccaatgt ttttttgaat caactctgca
    24721 acatatatta aagctttatc tcctcctcta actccataaa tgtcattaat taccttgaac
    24781 ttatctatat ccatcattat aattgcataa cgcttgtcag gatatctttt aagcattaaa
    24841 cttatttgtt cacaaaaact ttcataattg tatagtcctg ttaatgtatc tttttggttt
    24901 ttatgtagtt gcataagata atctatattt tttatagcaa ctaaaatatg tgtttgtttc
    24961 tcacctaact tatttttact actaatcttt aacatataaa ttaatgccca atagtacttt
    25021 ttatctattc ctaaacgtct atactcaaaa tttagttcta aatcgtcttg cttgctatta
    25081 ttaagactat caagagattt tagaaggtgg ttatcattaa ataatattat atcatcagga
    25141 tgaattaaat atttttttat gtatgaaaaa gttttttcaa gattttcctt tataggaaaa
    25201 ctaaaaatat tgctctctga ttgataaatt ggagtaatta aattcgtatc aatgcaaata
    25261 gataaaatta tatcatatgt attataagct aatttttcat aagctttaga ataccctttt
    25321 atcattaaat catcaaaata aaccttatca actaactctt tatgtttaaa taaaatatta
    25381 ttcgtcgaat cagctaagtc actaattact ttcatttcat tatttctaag tttgttctta
    25441 ttataatacc tcttcttatc ttggtacatt ctctcatcag caactttaat tagcttatgt
    25501 atatcaatat tatcgtcact ccaaatactg cccactgcta cactatatat catctcatcc
    25561 ttaaattctt cacttaacca actcactaat tcttcaaata gttgttttga tatatcttca
    25621 cataatacaa caaactcatc tcctccaata cggtaaacat tataagaatg aaagtatttt
    25681 tttattatta cacatgctga cacaatagca gaatcgccat tagcatgtcc aaagttatca
    25741 ttaatatctt ttaacccatt tatatctaca aatacaactc ctatggattt aacttttcct
    25801 ttttgaaaat tatctatata cttaatatat ttatttctat tatatgcacc agtcaagaag
    25861 tcatagtagc tcatataatt taatttattt tctacttttc tttttttcat ctcagttaat
    25921 aaaaaataag atagggatgt caaaagagag gtatctccaa cagctttatg tggattatca
    25981 actccaataa accctactac tttatcatct tgttttagtg aagatgtcat taaactttca
    26041 attttttgag gttttaaaat ttcatattct attggctgtt cttcttttat attttctata
    26101 tcatcaatta ttacaaaacc tttttgttcg aataataaaa accaattctt taattcatta
    26161 atatcaagat ttgttagtgc ctgtttttgt tcatcaacac cttcattgca ccattcatat
    26221 gtattagtta cttttgttct atcttctgat acttcaaaaa catatgctct atcagcttta
    26281 tgaaatttac caatattggt taatacaaga tttacagcat cagaaaaatt agattctgtt
    26341 ataagatgtt taatacatcc aactaataac tcctttatct ctaatttttc tgctattgac
    26401 ttacttatat cttccttttc tgtaatatca agagctattt ctaatcgtgc atgtttgccc
    26461 tcccatttaa ttaacttgtc ctttaatata aaatgtctat ttgttcgagt attcgtattt
    26521 tcccatatgt aatattcatc ttcttttaaa atagagtttg tacaaaattc gcagggagaa
    26581 tctttcccct gtaatgcttt ataacatttt aaatgggata tatcattaag tcccattgcc
    26641 tttttcccaa tttcattaat atataaaagt tcatatgtat ctaaagaaga aacatataca
    26701 atttcatcca actcatctat taaatttatt acatccattt atatatatct ccttttattc
    26761 attttccaga ttgaattttt aaagctttta aaattttgct atatatttgg caatgatatt
    26821 atacttcttt atgttttaaa atagtacctt aatgaggttc tattttttag taaatagaat
    26881 tattattgtg tcaatcgtac aatagagttt tgtaatactt aatttaatat atttataatt
    26941 aactatctta ttatgtattt acaattaatt ctcatataaa tgacaaatct ccacctcaat
    27001 taagtattcc aatcatatat gaggataata cttcattcat ttaaaaatat aaaactatta
    27061 tatattaaaa tttcttcaat aataaattat ttagattgtt actttgatat attatataat
    27121 atcacaaagg ctgatataaa aagacaaaaa gggcaatttt tttatatcaa aaaaaatgtt
    27181 aaattgatat tatccatata taaattgata ttatccatat aggtgctata tttaacaatt
    27241 ctacttactt cccttacact tattagcata tagttattaa gagtttgttg caaaacaaca
    27301 cttagcacac tttaaaaagt gtggtctaat aagtaatcaa cgaagaagac ataataaagc
    27361 ataaaatagg atatactttt ccacgtcatc aaaattgagg attttattat taatatatga
    27421 taaaatatgc ttaataaatt aaattttaat gggagaatag ttatgaaaat atttagtttc
    27481 ttgttgtttg tagtgggaat atgtttttgt attgctggat ttatggggca ttcctcagct
    27541 ttttctggtg caacattgag tttttttgca actacaatga tagctgtaag acaagtcaaa
    27601 actaagaaaa aatgaaatag gcaaatccta actttataag ttaatattac ataagtaatt
    27661 agtctaaaat tagatacttt ttctattaca aaaatacatc atccactttg tattctctta
    27721 aaaaaaataa aatagctatg taaattcaag attagataga ctaactctct atccatttca
    27781 ttgtaaaata tacatagcta tttgtattcg atttaaaata cttagatttt cttacataac
    27841 aaataatttc tatctgatta ttactcatgt tcatttatta accactcagc aactaatcat
    27901 ttagtatcta ttcatccgcc atacggtaac ctaccccaac ctcagtcaaa atatattctg
    27961 gctgagcagg attcttttca attttccttc ttatacttgc cataaaaact ctaagcgact
    28021 gattttcact ttcatagtaa tttccccaaa ctttatctat tataaactta tgggttaaga
    28081 ctcttcctga atacttagac atcaactcaa taatcttata ttcgattgga gttaaatgta
    28141 tttcttcacc attaattgac acaatatgat tttcataatc tatctttagg tttttaactt
    28201 caaaagactt tggagcatca ttatttatat tatcctgttg agctttattt ctaagagcca
    28261 cacgtactct agccaaaagc tctgttacac taaaaggttt agttaaataa tcgtctgctc
    28321 ctccatctaa agcttccacc ttatctctct catgctctct cgctgaaaca acaattattt
    28381 tattagtctt tgcacatgcc ctaactcgtc ctattacctc aatcccatca atatctggta
    28441 gtcctaaatc caaaattatc aaatctgggt caagagaatg tataagtgat atagctgttc
    28501 ctccattatc tgtctctaca cacctataat tttgagtttc aagagatact tttataaatt
    28561 tacaaattgg cttatcatct tctacaatta gaactaatgg ctttatttcc acttgtcttt
    28621 cccctcctca tcatacatag gtatgctaaa tctaaatgta gctcctccag aaggattatt
    28681 aaaggcgaca atttctccac catgagcttc tactatagat ttacatatct ccaatccaag
    28741 accaatacct cttctttcaa gatttctaga cttattctta gtataaaacc tattaaaaat
    28801 attcccgata tcttcatttg gtattccttt tccattatct gcaacttcaa atagtacttg
    28861 atttgactct ttttttacag ttagttctat cttagaattt ttaggtgtat gccttatagc
    28921 attatcaatt agatttacta acacttgttc tattaataat acatccacat gaacgattat
    28981 tatctcatct ggaatatttg tttttatgtc tctactattg gcaaactttt tgactcttaa
    29041 tatagattca gctattattt catcaacaac ctctggtcgt ttttcaatat ccaattttcc
    29101 ttcatctatt ctggtcatac taagtatatt ctcaacagaa tgagttagcc aactcgtatc
    29161 ttcatatata tttttcaata attctactct agtttcttta tctatcacat catcattttc
    29221 aagtattgtt gaagatgaac ccaatatact tgtaagtgga gttcttaaat catgagaaat
    29281 tgacctaagc aagtttcctc ttaacctctc tctctctgcc tcaaggtttg cttttttatt
    29341 tttctcaaat aaattttctc tatcaattgc caacgcaacc tgtgttgaaa ctgcttctaa
    29401 tagtatcttt tcattttctg taataatatc tatcttaaaa cacgcaacac ctataacacc
    29461 aagaacattt gattcttgac cttttatagg gaaataatat gcactgctgt ttttacaata
    29521 ttttgtccca attccaactg ggttacctgt cctaaaagct tcttttatag cttccttttc
    29581 taatactgaa tggaatatat tctcacttcc atcattgtta caaatataaa tacttggctt
    29641 acctagatta ttattctcat ctgcaatagt cactattaca gttctattta atatatttac
    29701 taaattagac ccacaaaatt caataatttg atttttattt tttgctttaa gcaacgtttt
    29761 gttattctca tagagcaatc ttatcatttt ttctctctta tgagaaagtc tcacttctct
    29821 ttttatccta gaagttaaag tacttgttat aattgctgcc attagcataa tacaaaaggt
    29881 cataggataa tcttttctat aagctaaaaa agtatataat ggatgcgtaa agaaaaaatt
    29941 aaatgatagc actcctatta ctgaagaaag tattccaaaa aaatatccat aggttatact
    30001 tgtagcaaat agtacaccta atatgtatac tagtataata ttagattcat taaaacctac
    30061 cttttcaaaa actactgata ttccagtagc aattgtcata accaatatag tttttatgaa
    30121 gtccttaata attatctttt tactgagata gccatcttca tcataatcat actcttttaa
    30181 gtcttcatgt tgtagtcgtt cctttttcat ttactacaat tcctccttat tgttactgtt
    30241 taaattggct ctattttata taatacaatt ttatcataat aaattcaatc aatacattta
    30301 attaatgtac ttaaattact aaaaggtgct gtcttaaaat gaaaataaat ctctatttca
    30361 aaacagcacc tatatattta aattactaat ttagttttta catacttaaa tcaatagttt
    30421 tattcaattt cataaattat aatacttcaa ttataaaaca tttctatcca acaagcataa
    30481 atataaagtg ggcacatata cctgcacata aaccacctat agtatgcgaa agtatcgctt
    30541 taccagccaa tggtcttgca tctaaagcat ccatcatacc tatgtgtgta cttaaatatc
    30601 cactccaaca catacccatt gctgtgaata ctgctatatc attaggagtt atagcgcctg
    30661 attttataaa ctctggtact aaagatattg ctgctccaac agctcctaat gcagttacag
    30721 ggaatgctat tgcctctggt gatgtgaatc caaataatgg ttctattatg aagctaatct
    30781 tatcaccaat agcaggtaat aattttatac cttcatatgc agctcctgtg taaacagctt
    30841 gacctgtagc agggtctgta gatggtccaa aagttaataa cataactaat gtacaaacta
    30901 ctaaaactcc tggtattata gccataccca tttcaacacc taacttacca ccttcaagta
    30961 tagcatccaa tgttctttgg aatacattac catcccttat ttctctaaac tcttcaagtt
    31021 ttgcaccttt atcatttatt tgcttttcag tatcattttt agggtcataa ttataatact
    31081 ttttagtaaa agtcaacata attctaacac taataatact acctattatt gccccaacat
    31141 tacctattat agctggtagt acatattctt taccttgagc tatcataaaa gttgttacaa
    31201 taagtcccat accaaaggct gttcctaaat tacataaagc aggtacttga tacttcttaa
    31261 aatattgtgt aaatgttttg tctttagcaa atggtataat agctggatta tcagataaat
    31321 atgttgcaac tactcctgct atactagcac caggtaagcc ccatataggt ctcataagac
    31381 ccttaaatat cttattaact aaagatatta ctccaaactc tgaaagcaat gcactaagtg
    31441 cacctgcaag aactgccata gccattatta aaaatactgt gtctaataaa agcgcatgtg
    31501 ctgtactcat tattacttta aacatcattc ctgcacccat gatgcttcct acatatccaa
    31561 atcctaccgc caatagtaca ataaatacga aggtttccat acttactgcc ttgactcttc
    31621 tcttatccga cagcttttcc atcttgaaat cttccccctt ttaattttaa attaaggttc
    31681 ctaacattgg tacaaatttt ttggtacaaa ctgagttcta aaacttacac tcgtaatgca
    31741 agttgtcagt tttttgaaat aaaaaaaaat tacgtttata gaataagaat atcctcttta
    31801 atttggatac tcttattcta ttttatgtta ttatattatc atccttttgt ttaaaagtca
    31861 attttttcta tttttgttgt ttaattttat ttatactctt tttatatttt tcattttctt
    31921 ttatctttgt aagtactcca tataagttga cttttctaaa tacagtagtt ataaatacca
    31981 atcctaatgc aattgaactt gcacacgata atgtactcac cccatattct acagctttta
    32041 tataattatt ggtcaaaaca gaatacatag tattatatat tccataacct ggcactaata
    32101 ttattaaaca gcaaacactt aatgtagttg ctggagtcat ataaattctt gcacataact
    32161 cacaatatat cgcaaaacaa actgtagcta taaaaaaaca taagttttct gttactccca
    32221 tatttaaaag aaatttatac gaaaaccaag ctatacttcc accgattcct gctagtatta
    32281 agttttttcc tttcatatta aatagcactc caaagcttaa ggcagtaaaa aaagctgcta
    32341 ttacttctat aaacaaatgc acttatatcc cccctaactt gaagtacaat ccaagtacca
    32401 agaacattcc tactgctata gatacaccta caaaaaatac ttctgctaat tttgcaagac
    32461 cagaaatcaa ctgtccatct aacaaatctc ttaatgcatt agtcaaagca agcccgggaa
    32521 ctaagagcat tattgctcct gctattaaat ggtctaacgt ctttatgaat ccgagcttta
    32581 aaattaaaat tgaacaagaa gctataataa ctgctccaat tgcatttaca aaaaagtcat
    32641 taagggaaga tttacttaaa tacataataa caaacttagt cagaatccca acagtaaatg
    32701 cagctataaa ttcttccaca tttcctccaa ataatatcga aaaagttgct gctgcaatac
    32761 ctgcaaaaaa tattgttatt aaatccgagt aatagttatc ttcacttatt tcatctaaaa
    32821 ttttctcaca taattcaata tctaaattat ttatacttat tgctcttgat aatgagttaa
    32881 cttcatgaac caagtttagg tctactcttc ttgagcttat tcttttaatt gctgaatgta
    32941 atttcccatc tacaaataca gatgccatta cagctgttgg agttgcaaat acatctacgt
    33001 ggtctattcc aaaagattga catattctag ctatcgtttc ttcaactcta taggtttctc
    33061 ctccactttg caacatagct ttacctgcat ttgaggaaaa gtttaaaatt ctatctatat
    33121 ccacaaaaca tatctcccat tcttttatag tattattcat attttaatac aagatattat
    33181 ttatttaaac tattttaaat aattcatatc caacatttac attttatgaa aaaacttgtt
    33241 ttttatgctt gtcaaaatca aattttaagc ttgtttacac cataaaatgg ctgaattaat
    33301 atttgacaaa cactttcaac catttatata accttctaaa ttgtataact ttttactaca
    33361 aataaaatta tatctctttt aattctttct cttgagtatt tagcttatag taacttcata
    33421 tttatatatg tacaatataa aacattattt ttatgataca aaatataatt tgtaaatgta
    33481 tatatgtatt aactgtgtat atataaatta ttcttaacct tatcagtttt ttcatatatc
    33541 ttcatctata ccgaccatat attaattaca ttgaccatat attaataaat gatattacat
    33601 agacttaagt atattaaaaa acttaaattt agttatttaa caacataata ctctaattta
    33661 atctataaaa atccatacct aatataatgc aactcttatt ttaacaccat ctattacaca
    33721 aaacaacata ttaaaaagta aaatacaaaa tatctactat aaaccttatt ttaatactga
    33781 atatatatta taatatattt aatgtagctt aaatatagtt taaacataaa gaaaggaaac
    33841 ggtatatgct aagaaaagat aaagttctat tatctctaca taaattatgt gaaaatatga
    33901 ctttaaaaga ccttcaatca gataaaacag gattcactac aatagaaatt gctaaccatt
    33961 catctcttga tagaagtaat accagtaaag aattaaataa cctatacaat gaaaaaaaag
    34021 ttctgaaaat atctggtaag ccaattttat ttctacatgc tagtattata gaaaatttaa
    34081 ttggccgtac tttgaacagt gatgaatttt ctataaataa ttgtaatgag ctacttaaat
    34141 caagtaaaaa tgactcagaa gatatttttt caacattact tggacatgat tctagtctta
    34201 aactacctat agaaaaagct aaatctgcta tactctatcc tccaaaaggt cttaatacat
    34261 tactaatagg acctactggt gttggaaaaa gtacatttgc tgaaacaatg tacaaatatg
    34321 ctgtagaaag taaaatcttt tcttctgaaa gcaaattcgt agtatttaac tgtgctgaat
    34381 atgcagaaaa tgctaactta ttattaagta atttatttgg ttatgtcaaa ggttctttta
    34441 ctggtgctaa taaggataaa ttcggcatag ttgcacaggc tgatggaggc atattattct
    34501 tagatgaaat acacagatta ccaccagaag gtcaagagat gctttttctt ttgatggaca
    34561 aaggcatata cagacgatta ggagaatctg acattgtgca taaagctaat gtctttattg
    34621 tgtgtgccac aacagaagat atacattctt ctctgcttgg aactttttta agaagaatcc
    34681 ctgttacaat caacctacct gctttacagg ataggtcttt aaaagaaaga ttatctctta
    34741 ttaaatactt ctttattata gaatcaaaat acaccaatgc aactataaaa gttcacaaag
    34801 atgtaattaa atcattactc ctttatgatt gttttggtaa tataggtcag cttaaatctg
    34861 acatacaact tatgagtgca agagccttct taaattacaa aactggactt aaggataata
    34921 tagaaataga tttatccctt gttcctgatt acatctatat tggtctttta aaattgaatg
    34981 aaaatcgtag taatctaaat aaattatcag aacttgatta tatgcttttt tatgaattta
    35041 atggtaataa cttagaagtt gaatatacaa aagaactatc taaatacaat atagatacat
    35101 caaacaatgg atttaaatac tctacagaga ttccttatct ggaaaatgat ataaggagta
    35161 tgtttataaa ttacatggaa aagtactcta caaaattact ttcgatatct tcagtaactc
    35221 attctgatta tacaagtaat gagattttta aagtaatcaa tccaaagatt tactatgctg
    35281 ttgaacattc tttaaaagag gctgaaaaaa tatcgagaaa caaatacaca aaacaaactt
    35341 atattgctct ttcaatgcat ataagtgcac ttatagaaaa tataaatcaa gacacatata
    35401 atcctaagta caatttatat aatgataaaa ttttcttaga taaagattta aatatagcta
    35461 aaatagtttt aagtataatt gaaaatgact tacaaacatc tttacctaaa gatgaagtaa
    35521 aatttatatc catgtttcta aatagcatgt atcttgaacc aaacaatcaa tctgaaagta
    35581 taggagtcat aatacttgct catggaaatt caacagcaag cagtatgtgt gatgtagcaa
    35641 attcattgct tggtacaaac cattgtaatg caattgacat gcctcttgat gtaaaagtaa
    35701 gtaccatact tgataaaact atagagttgg taaaagaatg tgacgaaggt aagggtgtac
    35761 ttcttttaac agatatgggc tctcttatag cttttggaga acttatttca gaaaaaacaa
    35821 aaatcaaaac tcgctcaatt gaaatggtat ctactccaat agttttagaa gcagtaagaa
    35881 aaagtacact accagaaatg actttggatg aattagctaa ttccttaagc tctttaaatc
    35941 catatattgg acgagcagca atcgattcaa cagaaataaa ctatcctgaa aagtcaagag
    36001 agtatgtaat aataactact tgtataacag gatatggttc agctatgaaa gtagcagact
    36061 ttataaattc ttcacttaaa aatattagta aatacaatat taatttagtt cctcataata
    36121 tagaaagttt taaaatgtat aagaataaat ttagtaatac taaaattctg gcagttgttg
    36181 gaagtgtaga ttttaatgta gctaacaccc cttttataca catagaggaa ctactaaatg
    36241 gatatggcct agatatgcta aacaaaataa tagaaggtac attcaatgcc aatagtatta
    36301 caaatataaa agaaacaaat ctatcataca acagtttaat aataaacttt atgacccaaa
    36361 acttagagct tttggactct gttaaattat tcaactatgt caattcttcc ttatttacaa
    36421 taatagaatt aatagatgaa aacttaaaga gcaaatttaa gctaggatat atactacatt
    36481 gctcttttat gatagaaaga tgttttaaaa atacaacttt tccatataat aacattactg
    36541 aattaattag taaaaatcat gaactatata gtttaatacg tgaagccttt aagccaacag
    36601 aagcttattt taatattttg ataccagacg aagaaattgc ttatataata gacatgatta
    36661 atgagtatct aaataaataa aatttatagc acttattttg tgcatattaa ttaatatatg
    36721 aaatggagga cgagattttg aaaggaaaaa taaaacagtt aacaatactt gcattaattt
    36781 ttatatttat aactcctgta tttgcatttg cagatactcc accagtacca aattcttcaa
    36841 gagcagcact acttatagac caggaaacta aaagaatatt atttgaaaaa aacattgatg
    36901 aaaaaatgcc attagcgagt ttaagtaaaa tgatgacctt tttattggca atagaagcag
    36961 ttgataaaaa tcaagttaaa gagactgata tggtgaaaat agacaagtct acagcttcag
    37021 taggaggttc tacttgtaaa cttaaagatg gtgatgaaat atctctaggt gagcttatgc
    37081 agggtctaat gctagtatct ggaaatgatg ctgctatagc catagctaaa catattggta
    37141 aaactgaaaa gaattttgta aatatgatga acaagaaagc agaagaaata ggaatgattg
    37201 atacgtatta ttttaatcca aatgggcttc ctatatatac tgacccagaa cataaagaac
    37261 ctcctattga aaatatgtca actgctcacg atatagttac acttggaaag tatatgtacg
    37321 accattatga aaatcaagtt acaagaataa ccactatgca agtatataat gatactaaaa
    37381 aggattttac acattataac acaaatcctc tgcttgtttc agtacctggt gttgatggaa
    37441 taaagacagg atatacagat aatgctggat attgccttgc attttctatg atggtaccta
    37501 aagatgctaa aaacgaaaga aatcatagat tgataggtgt tgtacttggt gatggtaaca
    37561 agaaaaatag aatttcttct tccgctactt tattgaaata tggtaaagat aatttccatt
    37621 ctaaaaagat agcacataag ggtgatatta tagagactcc ttgtgttgat ggtattgatg
    37681 attttaagat aacagttaag gttgataaag atttgtatgg tgttgtttca gataacgaaa
    37741 atatcaatcc taaggtagtg tttaaaaata tgaattatcc tatccataag ggagatattg
    37801 ttggtgttgt aaagtattat aatgactctg gtaaatttgt tggaagtgtt gatgtaaaaa
    37861 gtgaaagtaa tataggatgt attcctttga aggataagat taagattaag gttgctaaga
    37921 taaataaaaa acttgagatt aaaaattctg tttgttttaa agcatagtaa agcatattat
    37981 taaaagggta tgttaaattt ataaagactc cactttgtgg agtctttttg ttttttacaa
    38041 aaaattaaaa tagtggcaac aattgaggat gtgagggaga gttgccacta ttttgtatag
    38101 ggtattatat tatgttttta agctagtttt actatcttct atgtttatat aataactctt
    38161 gtgtgtgaat ttaattgata attaatatga aaattatgtg aagttttaag ttagttgact
    38221 tagactattg taaatttttt cagaaaaatt ctgttcataa gtctattaga ccaccgaatt
    38281 atttacctat aatattccga tcaaaaaatc tattcttttt taaaagtaag gtcatgatta
    38341 ttgtacatat ataaaaattc cgaaaattaa tctacacact agtaacatta agtataataa
    38401 atttatttta gtaaaactat taaaataacc tcataagaaa ctatagatta aatcaatcaa
    38461 tctaatctac agtttcttga ttattcctta atatttataa tttacattat ttgtttattt
    38521 taaaactatc tgttacaaat ctagtcaatg ttgaaaataa attcgctctg aatatagtag
    38581 atgatg
//
LOCUS       DAMJWS010000031        38460 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMJWS010000031 DAMJWS010000000
VERSION     DAMJWS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.031"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            94..996
                     /locus_tag="PC399_001688"
     CDS             94..996
                     /locus_tag="PC399_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2592550.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
     gene            complement(1195..1530)
                     /locus_tag="PC399_001689"
     CDS             complement(1195..1530)
                     /locus_tag="PC399_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592551.1"
                     /translation="MTHMSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVA
                     VWKPSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYS
                     CDLQQNKTT"
     gene            1480..1689
                     /locus_tag="PC399_001690"
     CDS             1480..1689
                     /locus_tag="PC399_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592552.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSRPNETDLIELKNAISK"
     gene            1782..4445
                     /gene="mgtA"
                     /locus_tag="PC399_001691"
     CDS             1782..4445
                     /gene="mgtA"
                     /locus_tag="PC399_001691"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF2592553.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            4461..4964
                     /locus_tag="PC399_001692"
     CDS             4461..4964
                     /locus_tag="PC399_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2592554.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            5102..5779
                     /locus_tag="PC399_001693"
     CDS             5102..5779
                     /locus_tag="PC399_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2592555.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            6026..6337
                     /locus_tag="PC399_001694"
     CDS             6026..6337
                     /locus_tag="PC399_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2592556.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            6503..9268
                     /gene="mgtA"
                     /locus_tag="PC399_001695"
     CDS             6503..9268
                     /gene="mgtA"
                     /locus_tag="PC399_001695"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF2592557.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            9281..9448
                     /locus_tag="PC399_001696"
     CDS             9281..9448
                     /locus_tag="PC399_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592558.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            10499..10798
                     /locus_tag="PC399_001697"
     CDS             10499..10798
                     /locus_tag="PC399_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2592559.1"
                     /translation="MKHLHIQQLSTIGSSLKKHLNSTEHNAPICGFLQHITGIICLAQ
                     DQYVCIALVLFYCIKLFQKIFAVGYQIPPFMVSVSAKRGRSLFALATFAGKGVRL"
     gene            10795..11211
                     /locus_tag="PC399_001698"
     CDS             10795..11211
                     /locus_tag="PC399_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2592560.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGAMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            11216..11455
                     /locus_tag="PC399_001699"
     CDS             11216..11455
                     /locus_tag="PC399_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2592561.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            11774..11917
                     /locus_tag="PC399_001700"
     CDS             11774..11917
                     /locus_tag="PC399_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2592562.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            11980..13740
                     /locus_tag="PC399_001701"
     CDS             11980..13740
                     /locus_tag="PC399_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2592563.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(14229..14867)
                     /locus_tag="PC399_001702"
     CDS             complement(14229..14867)
                     /locus_tag="PC399_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592564.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(15277..15453)
                     /locus_tag="PC399_001703"
     CDS             complement(15277..15453)
                     /locus_tag="PC399_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592565.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(15507..15603)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16400..16801
                     /locus_tag="PC399_001704"
     CDS             16400..16801
                     /locus_tag="PC399_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2592566.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            16798..17865
                     /gene="rsgA"
                     /locus_tag="PC399_001705"
     CDS             16798..17865
                     /gene="rsgA"
                     /locus_tag="PC399_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2592567.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            18401..19099
                     /locus_tag="PC399_001706"
     CDS             18401..19099
                     /locus_tag="PC399_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592568.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            19277..20167
                     /locus_tag="PC399_001707"
     CDS             19277..20167
                     /locus_tag="PC399_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592569.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(20560..21516)
                     /locus_tag="PC399_001708"
     CDS             complement(20560..21516)
                     /locus_tag="PC399_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2592570.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            22219..23214
                     /locus_tag="PC399_001709"
     CDS             22219..23214
                     /locus_tag="PC399_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2592571.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            23192..24055
                     /locus_tag="PC399_001710"
     CDS             23192..24055
                     /locus_tag="PC399_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2592572.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFLKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            24052..24846
                     /locus_tag="PC399_001711"
     CDS             24052..24846
                     /locus_tag="PC399_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2592573.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGIFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            25131..26510
                     /locus_tag="PC399_001712"
     CDS             25131..26510
                     /locus_tag="PC399_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2592574.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
     gene            26958..27671
                     /locus_tag="PC399_001713"
     CDS             26958..27671
                     /locus_tag="PC399_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2592575.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(27943..29481)
                     /locus_tag="PC399_001714"
     CDS             complement(27943..29481)
                     /locus_tag="PC399_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2592576.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            30363..30818
                     /locus_tag="PC399_001715"
     CDS             30363..30818
                     /locus_tag="PC399_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2592577.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            30867..31451
                     /locus_tag="PC399_001716"
     CDS             30867..31451
                     /locus_tag="PC399_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2592578.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(31456..31779)
                     /locus_tag="PC399_001717"
     CDS             complement(31456..31779)
                     /locus_tag="PC399_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592579.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            31931..32449
                     /locus_tag="PC399_001718"
     CDS             31931..32449
                     /locus_tag="PC399_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2592580.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(32625..33293)
                     /locus_tag="PC399_001719"
     CDS             complement(32625..33293)
                     /locus_tag="PC399_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF2592581.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            33408..34823
                     /locus_tag="PC399_001720"
     CDS             33408..34823
                     /locus_tag="PC399_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2592582.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            35046..35504
                     /locus_tag="PC399_001721"
     CDS             35046..35504
                     /locus_tag="PC399_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592583.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            35501..36085
                     /gene="clpP"
                     /locus_tag="PC399_001722"
     CDS             35501..36085
                     /gene="clpP"
                     /locus_tag="PC399_001722"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2592584.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            36397..37473
                     /locus_tag="PC399_001723"
     CDS             36397..37473
                     /locus_tag="PC399_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2592585.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            37540..>38460
                     /gene="bclA3"
                     /locus_tag="PC399_001724"
     CDS             37540..>38460
                     /gene="bclA3"
                     /locus_tag="PC399_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF2592586.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTG"
BASE COUNT        13330 a         5222 c         7001 g        12907 t
ORIGIN      
        1 acccgatagg atatacagac ctaagtagta gttactggca acagcactta atcggtgcag
       61 gacgagtaaa acaatagaaa ggacttgaaa aatatgttta agaagaataa gaaacagaca
      121 gaaaatgtca aagaaaaaaa ggtacgtact gtcaaggtag gcacacataa gaaaaccgtg
      181 attgcattgt gggtggtgct tattgcaagc gtgagctttg gagtatataa gaattttacc
      241 gctattgacc agcacacgac ccatgaaaaa gaagtgattg agcttcgctt gcaggacacc
      301 aacgggatag aaaatttcgt gaagaatttt gcaaagtcct attacacatg gagtaacagc
      361 aaagaagcga ttgacgcaag gacgcaggca atcagcggtt atctgacaaa ggaattgcag
      421 gacttgaata tggataccat tagaacggat ataccgacca gctctgccat tacagatgtg
      481 attgtatgga gcattgaaca gtctggaacg gacacttttt ccgctaccta cgaagtagat
      541 cagcagataa aagaggggga acagacaaca gcggttaagg caacctatac cgtaaaagtt
      601 catgtggacg ctggcgggaa tatggtaatt attcagaacc ctacccttgc accagcaatc
      661 gagaaatcag actatgaacc aaagacacca gaagcagacg caagtgtaga cgcggatacc
      721 ataaatgacg ctactgcatt tctggaaact ttctttaagc tatacccaac agccacagaa
      781 aaggagcttg cttactatgt gtctggaaat gtgcttgagt ctatcggcag ggactacctc
      841 tattctgaat tggtaaaccc tatctttacg gttgatgggg acaatgtgaa agtcaaggtt
      901 gccgtgaaat tcattgataa tcagacaaag gcaacacagg tatcgcagtt tgaattagtg
      961 ctacataagg agagcaattg gaagattata ggataattat tgaaagcgtg catttcttta
     1021 ggaatttgca cgcttttgtt aagagcatta ttctttatat ttatgaaaga catcttaaac
     1081 aaagtattaa tggcttcgca aaactttttt cttaatatat ttggattaga aaaagcgcat
     1141 cttaattgta gacttactat acatatgaat tatgccagtc aacgtattta aaatttatgt
     1201 tgttttattt tgttgtaaat cgcaagaata gataagagta atcaatggtg aaggggaatt
     1261 attaaaaata ctattggttt cttctgtccc gttccaattt gcgcattgta ttttttcata
     1321 aattacagag tggtcaatta tgtagttcag atattccata caaactaatt gtattgtttt
     1381 tacactcggt ttccatactg ccacttttgt cattctatca ctcataatac aaatattatt
     1441 atgagcaact tttctaagta gttcatttaa atttgataca tggtcaaggt gaaaaccaag
     1501 aagaagtttg tcagcactca tatgtgtcaa ggggaaatgc ctatttatgt gcgagagcgc
     1561 tatgagaaaa tgcttgccgt atccatccgt gctccaaatt tagaaggaat ggttgaaaac
     1621 tttgataagg atttatctcg ccctaacgag acggatttga tcgagctgaa aaatgccata
     1681 tcaaagtaaa ttgcaagaaa aaagcaagaa tataaatata gcattttcag aacactgtgt
     1741 taaaatgtta ttacttatac ttataaagga ggggatgttt cgtgtcaaaa gcaacactat
     1801 ttgatagtcg tatcaaaaaa tacgcatact gtagaacatc agaaatttat cgagacattg
     1861 gctcatctcc ggatggttta tccattgagc aaatcggttc aatgcgggag aaatacggag
     1921 cgaacagctt caatgggcgg aaaaatgata cgacgatgca acggttgaga cgggcattta
     1981 ttaacccgtt tcatgtgatt ctttttgttc tgggcattgt ttctcttgtt acggatgttt
     2041 tcgtagcatc caattttgcg agaaatgcta ctaccgccat tatcatcttt tccatgatcg
     2101 taattagtgg cgtaattcgt ctgattcagg agctgcgagc aaagagcgct gctgctcagc
     2161 ttgatcgcct agttcatgaa agcgtcacag tgaggcgtga tggaaagtta atagagattc
     2221 ctggcgaaga attagtggta ggggatattg tactattctc tgcaggtgat cgtgttccgg
     2281 cggatatcag attgacgaag attacggatc tctttatctc tcaggctgcg attactggcg
     2341 agagcgccat cattgaaaag agctgccgaa aactcagtta cggagagcag gatacactga
     2401 ctcagcttga aaacctcgct tttatggcaa ccacagtcat cagtggaaaa ggggaaggca
     2461 ttgtactggc agtaggcaaa gataccctgt atggaggatt taccaaaccg gattctgatg
     2521 ataaaaattc ctttaaaaaa ggagccgatt ccatcgcctg ggtgatgata cgttttatgg
     2581 cgatattagt tcccattgta tttatattac tggggattac tgatggaaaa tggatggaat
     2641 cttttgcttt cgccctgtca gttgctgtag gtctgatgcc ggaaatgctt cctatggtta
     2701 ttacagcctg ccttgccaaa ggaagtcttg ctatgagcaa aaagcagacc attatcaagg
     2761 atattaatgc catgcagagc tttggtagca tggatgtgct ttgtatggat aaaacaggaa
     2821 cacttaccaa tgaaagcatc ctgctggaat attatatgga tatttttggg aatgaaaatg
     2881 cagaggttct tgatcttgct tatctgaata gctgctatca ttctggtgtc tgcaatacca
     2941 ttgataatgc cattcttgcc tgcaatacca tgccagggcg ggaagcccat tacgcaaatc
     3001 ttctgacaaa attccaaaaa gctgatgaaa ttccctttga ctatgctcgt aagtttgtca
     3061 gtacacttgt acaggatagc aatgggaata gtcagcttat tatgaaaggt gatattgaac
     3121 atatattctc acgctgcaac catgtagaat atcgtggaaa acttctcccc atggggcagg
     3181 atgcgagaca gagtgtatct tctgtagtag gtgaaatgtt acaggacgga atgaaggtca
     3241 ttgcggtggc acgaaaaaat gtgggagcgc aaaagaaaat aactcctgat gacgaatggg
     3301 gcatgactct agttggctat ctatccttct ttgatgcacc aaagcagaca gcaaaggagt
     3361 ctgtaactgc actcaaaaga ttaaaagtta tccctaagat tctgacaggg gaccaggcag
     3421 cgattgctgt gtctgtttgc cgccgggtgg gtatttatac agagcgtatc ttaactggta
     3481 ctcaactgga tgaaatgaca gactgtgaac ttaaaaaggc tgtggaacat actcatgttt
     3541 ttgcagagct tactccggga cataaggtgc gtcttgtctc tgctatgcag gataatggtc
     3601 attccgtggg atttcttggt gatggagtca acgacattcc ggcactgaat gaagcaaatg
     3661 tgggaatctc tgtagatacg gcagtggacg cagcaaaaga tgctgccgat gtggttttgc
     3721 tgcaaaagga cttgaatgtt ttggagcaag gtgtaatgga ggggcgaaaa acttttacca
     3781 atatgctgaa atatattaaa atcaccgcta gctctaactt tggaaatatt ttttccatca
     3841 tttgtgccag tgccttttta ccgttcctgc ctatgacttc aattcagatt ctgcttttga
     3901 atctgctgta tgatacgttg tgtattgtgc ttccttggga caatgtagat gaggaagaga
     3961 tgctgtcccc aagagactgg tccggcaaaa cactgagaca gtttatgctg tctttcggac
     4021 cgatcagttc tctgtttgat attgtcacct tcctattttt gtattatttc ctatgtccga
     4081 tgctatgtgg aggggcaacc tatctgcagc ttacagatcc ttccctgcaa cttcagtatg
     4141 tagcactgtt tcagactgga tggtttttgg aatctatgtg gacacaggtt ttgattttac
     4201 attttctgcg aacttccaag attccttttt tccagagcag ggcatctgca ccggtcattt
     4261 ctataactct agtggggatt attgctttta cttcccttac atttgtcggc ggtgcgtcgt
     4321 tgtttggact gacgaaatta cctctttggt atttcgtatt tttactgctg gtggtatctc
     4381 tctatatgct gctgaattca gtagccaaat atttttataa gaaaaaatat caagagttga
     4441 tttaagagga ggcattcatg atgaaaaaga atattagtta tattccgatg gattcggctc
     4501 ttgaaatctg gctattagaa aaactactaa gagatccacc ggacagtgat tgtgcgaagg
     4561 atttactttc tggtattcag gagatgattc gtggagaggt atcttcgtta ttgttaggag
     4621 catcagaaga aagcgattgg ccagtgggta ctctgtcaga tagaatcctg gcaggggatc
     4681 tggaaattta tccaagaggc agaaaagtac tgataaaagg ttctgaagtc aacctgactc
     4741 caaaggaatt tgatattttg tactttcttg tccagaacaa aggtgaagtt tttacaaagg
     4801 aacagattta tcgtgccgta tgggaggaag attatattct ggataacagc aacattatgg
     4861 cttttatccg gaagttgcga aaaaagattg aacctgctcc ggattgtccc acatatattt
     4921 tgaccatctg gggtattgga tacaaattta atgatcagat atgacaaaga cggactaaga
     4981 ccattttcgg tttcagtccg tctattttct taccaaatcg caagaaaatc gcaagacttt
     5041 agccatagga ttgtatttgg attttttata tactgttagt agttaaaggg aggtgtttca
     5101 tatgttttac acagatttta ttttgagaat tgccctctcc ttaacacttg gatttcttat
     5161 tggattagaa cggcaattga ctggtcatcc tgcaggtatt cgtatcaatg tgttaatctg
     5221 catgggtaca agtttcttta ccttgtttcc tatgctctac ggttcggatc aggtattccg
     5281 tgtcggaagc agtattatat ccggtgttgg ttttttatgc agtggtgtta ttttcaagga
     5341 cagagggaca gtacgtggaa tgaataccgc tgccacctta tggtgtactg cagcgattgg
     5401 aattcttgca agtacgggaa tgtatgcaat ggcgatcacc gccgctacta ttttaattgg
     5461 ttccaacctg attctacgtc cctttgcaag aaagtttcac ccaattactt ccagtgatga
     5521 atctgaaaag cagtatcgta tttcggttac atgtcaggaa aaggcagagc aggatatacg
     5581 cttattgctg attaacagca attcctgtaa gacgctcttt ttgaacaatc tggaaagcgg
     5641 tgatgtggtt ggtgacaagg ttgagattgt cgcagaatac tgttctgtcg gaaaatccaa
     5701 aaacaatatt ttagaaggaa tcgttgggca ggtattggta attccggaag tgattagtgc
     5761 tggttgggag gtgttgtgag tgaggagaga aatcgaagat ttgaaacgag caattctttt
     5821 tgctttctac cctgtattta caatcttatg tagctatttg ggttggaatt tctttgaaca
     5881 aagaatggta atctttaaga tatggtatca ggtgagtatt gtgatctggt ttattcttgc
     5941 tgttactatt attgtgctct tcattaaact tctgaagctg acaaaactaa gaaacaggtt
     6001 taagcattga aaggaggaac aaacgatgga ttttacatgt atcttttttg gaattttatt
     6061 tactcttgca ggcttttggt tggcctttgg aaaaggatat cgtcatcttt ccctgtggaa
     6121 aaatatgccg caggaggaga aagataaaat taatattgtc ccgctttgtc gtaatgttgg
     6181 agaaatgatt gcgttaaatg ggattatatt cttaatgaaa ggatttttgc cgggattttc
     6241 aaaccactgg tttgtatttg caataatcgc atggctgatt gttgccggct ttgatgtctg
     6301 gtatatcgaa aaaagtgtcc gctatcgcaa ccaataatcc acattgaggt ttcacataga
     6361 tattttttta agattgtgag gtgaaagaca tggactctga tgtcagtcca cgaaggcaga
     6421 gaatattata tgagaatttg gagattccgt gtcgctcata taatattcct tgcccctatg
     6481 aaaattcaag gaggtaagac caatgaacaa aaaagaaaat cgtatggcta ttcgtcagac
     6541 tgtagaaaaa gcagtcatcc gtgacgaaca gaaccgccgc attcaatttg cggcaaccaa
     6601 tcctattaaa gaagttttaa aaaaacttca taccacactt cgtggcttag aagtagatga
     6661 agtatccgta agtcgtgcta aatatggtac caataaagtc actcatgaaa aaaagaaatc
     6721 tctggcaaaa cgactggttg gcgcatttat caatccattt accgccattt tattttgtct
     6781 ggctttagta tccacaatta cagatatgat ttttccgtat ttttctctgt ttggcagtgt
     6841 gccagaggac tttgattccc tgactgttgt aatcatttta acaatggtat tcatttccgg
     6901 tacgcttcgt tttgtacagg aatccagaag cggaaatgca gcagagaaat tgttagccat
     6961 gattacgacc acttgtacag ttactcgtag aaactatgaa aaaaaagaac ttcctatgga
     7021 tgatttggtg gtgggagata ttgtgcatct ttctgctgga gatatgattc cggcggatgt
     7081 tcgtattttg gatgcgaagg atttgtttgt cagtcaggca agtcttactg gtgagagtga
     7141 acctattgaa aaaacaccca atgtaagtgc tcagaaagaa agcgtgactg attacacaaa
     7201 cattgctttt atggggagta atgtaatttc tggtagcgca acagctgttg ttgtttgtgt
     7261 tggcgatcat actctgtttg gctctatggc gtccgcagtt gctggagaag cggttgaaac
     7321 aagctttacc aagggtgtca atgccgtttc ttgggtattg attcgtttta tgctcattat
     7381 ggttccgctg gtgttctttg tcaatggtat caccaagggc gattggttgg cggccttctt
     7441 attcggcgtt tctattgctg ttggtttgac accagaaatg ttgccgatga ttgtaacaac
     7501 ctgtcttgcg aaaggcgctg tttccatgag caagaagcag acaatcgtta aaaacctcaa
     7561 ttccattcag aacttcggtg ctattgatat tctctgtact gacaagactg gcacgctgac
     7621 ccaggataag gttgtgctgg aataccatct gaatgtaaat ggcgaggacg atacgcgagt
     7681 actgcgtcac gcttacctca atagttactt ccagactggc tataaaaacc tgatggattt
     7741 ggcaattatc cataagacgg aggaagagga agccgcagat tcgcgtctca ttgacctgtc
     7801 tgaaaactat gtgaaagtag atgagattcc ttttgacttc acacgtcgtc gtctgactac
     7861 agtggtacag gataataagg gtaagaccca gatggtaaca aaaggtgctg tggaagaaat
     7921 gctctctatt tgtgcgtttg cagagtgtaa tggaggagtg cagcctctga ctgatgacgt
     7981 tcgttacaga attctggaga cagtggacaa tcttaacgat aagggattcc gcgtactagc
     8041 tattgcacag aagagtaatc cttccccaat cggcgcattt ggtgttaaag atgagtgtga
     8101 tatggtactg attggatatc tggcattcct tgatcctcca aaagaatcta ctgcggatgc
     8161 tattaaagct ctgaaagacc atggtgttat aaccaaaatt ttgacaggtg acaacgacaa
     8221 agtgactcga actatctgca agcaggtggg attagaggtt cgcaatatgc ttctcggttc
     8281 tgatttggaa caaatgggcg atgcagagct tgcaagagtg gcagagtcta cagatgtatt
     8341 tgcaaagcta acccctgatc agaaagcccg tgtggtttct gttctccgtg aaaatggtca
     8401 tacggtcggt ttcatgggtg acggtattaa tgatgctgca gctatgaaag ccgccgatat
     8461 tggtatttcg gtggataccg ccgtggacgt ggcaaaagaa tctgctgaca ttattctatt
     8521 ggaaaaagat ctgatggttt tggaacaggg tattattgag ggacgaaaga catatgccaa
     8581 catgatcaag tacattaaaa tgactgcttc atccaacttc ggcaatatgt tctcagtgct
     8641 tgctgcatct gctttgctac ctttcttacc aatgatgagt gttcatctta tatttctaaa
     8701 cttaatttat gatttatcct gtactgcaat tccttgggat aatgtggatg aggagtttat
     8761 taccaaacct cgtaaatggg acgcttccag tgttggaagt tttatgatct ggattggacc
     8821 aaccagttct atctttgatt ttacaaccta tatttttatg tactttgtat tttgtccatt
     8881 gttcgtatcg aatggtgtat tatttaatga tttgcctgct catttcagcg gtgcagaact
     8941 ggcaatgctt caggcacagt atatcgggat gttccaagca gggtggttcg tggaatctat
     9001 gtggagccag acattagtca ttcacatgat tcgtactcca aagctacctt ttgttcagag
     9061 tcgtgcatca gctccggtga ctttgctaac aatgactgga attatagttc tgaccattat
     9121 tccgtttact gtgtttggaa ctatgcttgg atttgttgca ttgcctgcag catactttgc
     9181 ttatctgatt ccatgtattc tgctgtatat gactttggca acaagtttaa aaaaagccta
     9241 tgtccgtcat ttcggagaac tgctttaagg aggtggaatg atgaactgga aagaactttg
     9301 gatgaccctt tttggaacta ccgaatggct tggtcttaat atcggctttt gggtggctat
     9361 ggcagttgtg ttgctgattg ttattgtaat gaatgtagta ttttggtcca tgaaaccaaa
     9421 aaaagacaca aaagagtctt ataaataaga tatcagtatg aatgaatcaa gggagatgca
     9481 cagtttttta ttgtggaatc tccctatcat atcaaggcgc attcaattgt ggcaatttag
     9541 tagtaaagta tgtgtttttt tatcattgaa ttacctataa atgctgtata taagtggata
     9601 attgtaattt cgatataaaa attatggtac ttaaaagaaa tttcgttttt tattgtttgt
     9661 ttacgcgtct ataacgaaat cgtgaaataa tattcaaagg tagattttat tatggaatgt
     9721 atgtctgtag aagaagcttc gataaaatga ggagatatct gaaagaagga taaaaaaatt
     9781 atgtaatgga aacagaatcc caaatctatt gtaacaacaa tgacaggtaa tgaatggtta
     9841 cgtagattga taattcctat tcaaattatt atctatctta tattgagtgg tgttttgtaa
     9901 ttacattttt atacaaaaag aatatgttga acgcacaatg ataaatattt tgtattcccc
     9961 aactaataga ataaagtttt tgcttggaaa gtatgttata taggcattat ggtttgtaat
    10021 gatcacgttg acagattata ccgctatata tgaagtcaaa gaacttaaat catttacaag
    10081 tgagataatc tatctaagtc taaaaaactg gatttctcaa tctattgtca atgagtccat
    10141 tacttattat ttgtgttttg caaagaaatg ttttctactc tagtttactt tttagttact
    10201 aaatatcggg aattggtttt gttggactat attgatctga aagtatacaa aatattattc
    10261 cgtggtctgc tgtaacccct ataagtttat tagatgcaca aggattgcta ccttatattt
    10321 caattctact ttgttatttt gcaagactga ttataaatac atactgcttt agagggttta
    10381 gtttagttct ttttgaactt ttgaataaga cgacggttat atagccgccg ttttattttt
    10441 gcctaacgtt ggacaaatta ctggatggtc ttgttttatc ggctctaggg gtagccgtat
    10501 gaaacactta catattcaac aactttcgac catcggtagt tcattaaaaa aacatttaaa
    10561 ttctacggaa cataatgctc ctatatgtgg ttttttacag catataacag gaataatttg
    10621 tttagcacaa gatcagtacg tctgtattgc ccttgtgctt ttttactgca taaaactttt
    10681 tcaaaaaatt tttgcggttg ggtatcaaat cccacctttc atggtgagtg ttagtgcgaa
    10741 aagaggtaga agtcttttcg ctttagcaac tttcgctgga aagggggtga gattatgaaa
    10801 ccatcttctt tcgagggcgc tatcagactt caatttgatt gtctagctcg caaggttgta
    10861 ggacgaactg taaagaacta tgaccgagag cttggcagac gtgcaagacg tgaaacgccg
    10921 ttctgtgatt tatctgaaat ggagttaaac cagatcggtg ccatggacga atattgcgtt
    10981 gagtttacgt catttgatgt atttggttca gaagtccata tctatgatga acgcttatgt
    11041 gaagctatca aagaattaac agaaagccga cgtaacattc tgcttatgtc ctactttctt
    11101 gaaatgaccg acgctgaaat tgcagaggtt atggaaatgg aacgattttc cgtatgtaga
    11161 aacaggctgc acacattaaa gcttattaag gatatgtacg aggaggaata aaaacatgat
    11221 gaagtctaca aaaaagtgtc catcattctc gaccatcagt tctgcagctg acggtaacga
    11281 ggtggcaatt gaaaagattt tgaaccatta tgacgcttac atatcaaaag ccagcctacg
    11341 cccgttctat gacgaacacg ggaatatgta tatcgtgctt gatatggaac tgaaaggcag
    11401 gatacgggca gcgttgatta aagtaatctt agggtttgaa gtcaaagtga agtaaatata
    11461 acagagagta tctaccacct cattttaact cttttccgtt ctttgacaag tgaatacagt
    11521 aatcagatac gtttgatata cgacgagcca gcggactgga acgccacgac cactgaaatg
    11581 gggaaagcag cgagcgatta tgccagtgaa ccgcaagcga tctttggaaa ggttgctgcc
    11641 atgactcgta tatctgaata atgatacacc cgtatgacac ggttcaccca tcagaatggg
    11701 aatggtgaaa gtccagtgga gctgccgaag ccgtctgatt acttccataa ataaaaaagc
    11761 aaggagggtt ttaatgaata atataattag gtatagtatg caaattgcta tgttaaaaca
    11821 actgcttgcc cgtaaactga tttctgaaaa agaatacact cttgtaaaac aaaggctgat
    11881 gaaagactat aaggtgcttt ctgacataac tgcttgaatt gtgtttttta aagtagtaca
    11941 ataacaatat gaaaggaaca caaaataagg aggtgtacta tgcaggaagt tgaagtaata
    12001 aaagcaaaaa atgttttggc aagacaagcc cgtggaaaag ccattgagcg tttgcgtgta
    12061 gctgcatatt gccgtgtaag tacagatagc gaagatcagt taaacagtta taaatcacaa
    12121 gttcagtatt atactgacat gattaagaaa aacaaggaat gggtgttagc tgacatttat
    12181 gctgatgaag caattacggg gacacaagta acaaaacgag aagattttca gcgtatgata
    12241 aatgactgta tgaatggtga aattgacatg gtatttacaa aatccatatc aagatttgcc
    12301 agaaatactt tagacacatt gaaatatgtt cgtatgctaa aagagagaaa tatcgccgtt
    12361 tattttgaag atgaaaaaat caatacactt acaatggatg gagagttatt gcttgttgtt
    12421 cttagttctg ttgcacagca ggaagtcgag aacatttcag ccaatgtaaa aaaagggttg
    12481 aaaatgaaaa tgaagcgcgg cgagctggta ggttttcaag gttgtattgg atatgattat
    12541 cacccagagg ataagacaat aactgtaaat caagaagaag cagagattgt cagatatatt
    12601 ttcaaccgtt atgttgatgg tgctggtggt tctgttattg gacaggagct tgaaagtcta
    12661 ggttataaaa cgaagtatgg gagttccaca tgggcaccat ctacggttat tggaattatt
    12721 aagaatgaga aatacaaggg tgatatactc ttaggcaaaa cttttactgt tgatccgatt
    12781 tccaaacgtc gccttgaaaa catgggtgaa gaagataaat tttatatgaa agatcaccat
    12841 gagccgatta ttagtgaaga agtttttgaa aaagcacagg aaattcttaa tcggagaaat
    12901 aagaaccgaa caacggtagg cagcggtaag cgtgagaagt atagcaggaa atacgcattt
    12961 agctgtatgc tagaatgtgg cttttgtggt ggtactttaa caagacgtaa ttggcacagt
    13021 ggctcaaaat acagcaaggt tatatggcaa tgtgtaacgg ctacgaagaa aggaaagaaa
    13081 ttttgtaaac atagcaaagg tattccagaa acagctattg aagaagcatt tgtagaaagt
    13141 tatcgtttat tatgtgatga taataaagat gttctggaag aatttttgca gagaatggac
    13201 gatactctta gtagtagtgt ggtgtcaaaa caacttgcaa aagcggaaaa ggagatagac
    13261 gcattagaga agaaaaaaag taaacttgtg gatatgcgtc tggaagaaat tattgataag
    13321 gaaacctacg aaagtaaata tgctgattta gtaagtaaac aagaacagct tgttgaagaa
    13381 agacaaaagt tacaagaaac ttctgataat gagaaagaca taaaaaaacg tttgaaagag
    13441 tttaagaaaa ctctggaaca gaatgaggtt cttgataaat ttgaccgcta tgtgtttgaa
    13501 agcattgttg aaaaagtaat tgtaggtgga cttgatgaaa acggaaatgt tgaccctgca
    13561 cagttgacct ttgtttataa aacaggcttg aagaatagcg tagatggtgc taaatttaaa
    13621 acacaaagga aaaatgcaag aggacggcat aggactaatg aattgtgttc acatgacagc
    13681 aacgaggttg ataaaatgtg ttccgatagt agtaacgaca catgtggaaa ctgtagctaa
    13741 gataatcaaa caatagtaat gtttatatag attatatact aataatatat tgtatttgcc
    13801 accgatttgc cactgtaaag cgatttcggt ggcaaattta gtctacaatg ttatcaaaaa
    13861 tctctaaagt tttattttta attctataaa acttattata attgtaattt taaaagaaat
    13921 agtcaatcta cagtagccag attttataca actcattttt ttcatagagt aatttacata
    13981 atctatagtt gaatattatt taatattttt ataattcatt agcaaatata ggcattacaa
    14041 atgttgataa acatgtgaaa gcacatatcc acttataatt atttttttta aaaccataaa
    14101 atcacctctt catttgttgt atgtttttat aacaaatgaa gaggtgattt ccagacaatt
    14161 taaaaggttt ttgtatattg actaaaattt atttttatta tttataattc ttaataatac
    14221 atatgtacct actgattcca tattaaactg tataattttt gttcatcttt ttttataatt
    14281 aaaactacgt agttctcata atttctctta tatatatact tatcatcttt tttaacttct
    14341 aacttatatt ttttaaaatc ttcttcaatt ttatttttat tacttttatt aatgttaaca
    14401 gaatctcttt taaatttatc aatatatttt tctatttcat taatattatc tttgcttaca
    14461 actataccac cttttaaatt aaaaactttt ttgaaatttt tttcagaaaa tccttgaata
    14521 aaataacttt gtccatctcc attaatacta tcttgagtat caaatacttc aatttctttg
    14581 ttaggtattg gtatatcaaa attccaagat tcctttagca ttgaaatatt agctgttttt
    14641 cctaaaaaaa gattatatag gatacttcct aataacaaaa cacctattaa tagtagtgtg
    14701 attaatacaa tttttctctt tttcattttt cgttcacaac cttttgaaaa aacatgattt
    14761 aattcatcat ttgaaggcat gtcttcaaga ataatttttg ttgtgatagg aatgctttct
    14821 tgaaacatat catttgtaat atcaaaatct tttctttgat tactcaatat ccatttcttc
    14881 taatatttct tttgattttt tctagtacat tgaatactct tatatacatt ctcctctttt
    14941 aaaattaaaa gttataaaag aataagttat ataaaaaatt attatatttt ttaatatagc
    15001 ttatttgcaa atttataatt ttgttaatct aatccttcaa actaaaaaat catttatatt
    15061 tacagaattt cattttaaga gatgtttatt ttcacataga taagagaatt taagttctca
    15121 tatttattat ttgatacaaa tttacatctt tattatatgt attttaaata aaaaataaaa
    15181 ttatgaatac tagaaatcta tcttaattag ctttctaaca taaggctatt taaagtagat
    15241 aaaactaaaa agggttctct agaaaaccaa atctatttaa ttatacttac ttactaatac
    15301 catcacttga tgcaaacaag attctaccac attttctccg tactctagta taaaatcatc
    15361 tagttgtgta tctataaaat cacaataatc atatgaacta ccaatataag cagtcaacat
    15421 atgtgtacat ctatccattt tatctctaaa catatatatt attatccccc taaaaaactt
    15481 tcataaaaac aaaaagaata tctctttttg gcaactggct gacataactc atagtttatt
    15541 ttataagcgt tagtccctat agctttgcgt cactaaattt ctctagtttt gccgatttag
    15601 ttttattcta caattacaat aatacaatag aattgcttat tattcaatat acttatttga
    15661 aaattaaata aatttacaaa tatgaacgtt attttatatc aaaaaaaata gactatgcat
    15721 gtagaatata tggaaaaact tgattgtagg ttttgtatga aaagacagtt gttattaatt
    15781 taagtgagat aaattgagca acaggcataa aatgagaaaa caaataatag cagttaattt
    15841 ttattattaa tactaggtga acattaaatt tcaaaatacc actagatttt atagaataag
    15901 aaaatgagac aaacataaat atatgaatat attaaattat acttgaacat acttatattt
    15961 tatgataaaa tttcaataat aattattagt gaaggagtgt cttgcctgat gtcagagtta
    16021 tatataatgg taaccaacaa ataaatagtt ggagacgcat atgggttctg tttaatcaga
    16081 atttttaagt gtatttttga tacatttgat tgttgtgtct tactctgcct ttatagataa
    16141 ctaacattag acaacaaata ataatattac atattacttt gttaaattgt ggtgtagtgg
    16201 gctttaatct gctacacttt tattaatttt acacagtatt attttgaata taattatgtt
    16261 gaaatagaag atactaaatt ttctagtcca caggggtttt ctgtttttgt gtaggtaaat
    16321 atgtatacca cataaaaagg cagatataaa tacctgtgga ctttttttgt agaaaataca
    16381 cgatacggag gaaataaaaa tgaattataa aaatagaaaa cgagcttatt atcaggaaaa
    16441 ttcctgtatg gataacttta tttgcaaaaa ttgtggttgc cttgtagttc ctgaaggagc
    16501 tggtacacaa catagaaatc attgtccaca ttgtttacat agtcttcatg tagatatcat
    16561 tcctggtgac cgtgctgcag attgtgatgc taatatggaa cctattggag tgtgggtgag
    16621 aaaaaatggt gagtgggcaa ttattcatcg ttgtacacgc tgtggacatc ttagttctaa
    16681 tcgtattgca gctgatgata attcagtgaa attaatgtca attgcattaa aaccactggc
    16741 acagccccct ttcccactag aaagaatcat tgaaaagttg gaaaaggagg aaacagaatg
    16801 aatggtatga aacaaatcaa tttacaagac tatggtctaa atagaaattt tattactcaa
    16861 agtatggaat atcatgattt ataccttgct cggattatct cgcaggaaaa gaacttttat
    16921 caagctgtat gtgtaaatgg tgtactgttt gcagaggttt caggaaagct gcgttttgag
    16981 gctactatac aatctgattt tcccacagtt ggtgatttcg ttatgttgga tcgttgcaat
    17041 aataaaaatg gtaatgctat tattcatcat gtgcttcata gaaaaagctt gtttgtgcgt
    17101 aaggctgctg gaaaggtaaa tgaggagcag gtggttgcag ctaatattga cattgttttt
    17161 ttgtgcatgt cactgaataa ggattttaac attcatagat tggagaggta tctgtcttta
    17221 acatgggaaa gtggagctat cccagtagta gtacttacta aatcagactt atgtgacaat
    17281 gtgcatcgta aattacaagt agttaattct atagccttgg gtgtagatgt agttgttacc
    17341 tcatctatag agccagatgg ttataaacag atttctaagt atattaaacc aggctgtact
    17401 gttgccttac ttggttcttc aggagttgga aaatcttcat taattaattg tctgatgggt
    17461 ggagaatttt taaatacaaa ggaaattcgc atagatgatg ataaaggaag acatgccacg
    17521 actagacgag agctattttt aatttcaggt ggtggtattg ttattgacac accaggtatg
    17581 agagagttgg gaatgtggga tgtttcagaa ggtttagata aaagttttgc agatatagaa
    17641 gcttatttcg gaaaatgtca ttttcgtaat tgtactcaca cctcagaacc aggctgtgct
    17701 atctgggaag ctattgctca tcaagaactt tcgcctaaaa gatgggaatc ctatcgtagg
    17761 cttaaaactg aagcagtatt tgcagacaat aaaagcgaat acttagcaaa aaaagaaaag
    17821 aaatttaaaa atatctcaaa aataaataag aagattagaa gataagtgta gtttttttgt
    17881 tcattctatg agtctatata ctattaatat attaaatttg ccatcaattt gttactttaa
    17941 tgtgattttg atggcaaatt tggtttgtgg tattatttaa agcaatttat taattttttt
    18001 aataaagttt gattttttat atgatgatat atacatgtga taatgtatac atatttgtta
    18061 cgataggctc ctttataaag aggtcttatg taagataaga aaaggcatat ttatactaat
    18121 acataataga gtatatgctt atgtatgata gagatgtttc ttaagagcaa aactgcaata
    18181 ttgctaattt ataaagtaaa aatatatgaa ttataaaata caaataatat atttataaaa
    18241 attattgttt taatttataa taaaaatttt ttgttgaaaa taatgctcga acctataaaa
    18301 ctattaacta atatttgtaa atgtatactt ttgatgataa aatataatta agttaagttg
    18361 gtttttataa acataaattt attttaagga ggtgaaataa atgaaaatat ctagtcaata
    18421 tagaagtcaa tattcattta gatatgaaag taatataaat aatacaagaa taaatgaaag
    18481 tatggttaag aaaaatgaaa ctgtaggaaa agacacttat ttatctaata ttatgaaaca
    18541 aaagcaagaa cttaatgata gaattagaga tttaaaatat agacaagagg tttatactaa
    18601 aaaaataaat gacgcaatta agaacttatg taaatcagaa ataagagaaa caactaataa
    18661 tttttctaat atagaaatag gtattaaaaa tagcattata gaagagaaaa ataaaagtac
    18721 aatgttagat gaaaattcaa cttatctaaa tacaaatgat gaaaaagaat ctttaatcac
    18781 taaagagtct aatgaaaaaa ttgaagaaga aatattaaat gatgaaaaat tagaagagtt
    18841 agaacaaaaa aaggattata aagaggattc taataaaaaa gagaaagtat ctgaggactt
    18901 atctttagta ggtaaaactc gtgaagagct tgaaaatatg cttaaaaatt ttataaattt
    18961 aacacaagaa gaaataatga aacttgagtc gagaatagaa aagttagata aaaatgctga
    19021 agaatacaaa caaaattcaa agactaatat atttgataaa acagatgaac aaaaaaaaca
    19081 tataaatgta ctgatttaaa caatatgttt taaaaaaata agtttattat ataatgaaaa
    19141 tataaaggta caatttcaat acacttctat taaaccaata atttatgaat cagatttaat
    19201 ttcaatttac taataaccat atatttatga gttaataatt ttatgtttta aaaagaatta
    19261 aataaggggg agtagtatga agaagaatat attaactact atgattacca tgattattat
    19321 attgatgatg agtatgacag taatggcaga gccatatcaa gaagatgaaa catctagctt
    19381 gaaaaaacag agtgcaaatg aacgtaatca gactaatata ggcacaatgg atggcagagt
    19441 gcaaacaaat gcaataagta aaggtgctaa ttatgaccct aaatatcctc ttaaaggaat
    19501 gttaacacaa cttggtctta atacaacagg taaagaggga tgggatttac tctatggagg
    19561 aaatcccaat ggagttgagc ttcgagcaga tggtaaatat tattatataa ataagggtat
    19621 ggaagggcta ggattttatg cggcagcatt gtcaggtggc tcatatcaga gaataggctt
    19681 tggaaaactt gaccctatag aagtaatagc tgatggaaat tggatttctt acaaacaagc
    19741 tcaagataca cttacagtaa tcaggaattt tttaaatagc tttgactgga gaaatgcatc
    19801 tgagatggaa agagctaata gagctgcaaa attggtaacc gaagcaaaat atgcagactc
    19861 taaatattgt aacatagtct atggtaatct ggttgataaa cgtggtgtat gtggaagttt
    19921 tgcaagtagc tttcatttat taacaaggtt aatgggaatg gattcactat ctatattaaa
    19981 tccatcatta aatcatgctt ggaactatat acaaattgat gggaaatggt ataggtttga
    20041 tgggtctgaa atatcagctt ttggtggatc attagacttt gattaccgta aattaaagga
    20101 tgcaacaaga gagatgacta cttactatga tgctaaggca ttgtctatat taggatttaa
    20161 tcaataaaaa tagggggagg cttcttatag agcctttttt tattgatttg aaatttaata
    20221 aggaatttta tatgaaaatt taatcaataa aagcaaaaaa ggctctttat aaagcctttc
    20281 taagtgtttt tttaagtaaa attttttata tcaacatcag ttttaatcaa aacatataac
    20341 attttggact tacttttaaa aataaaatta ttaaaatata atattaatca tgtttttagt
    20401 ttattatatt ctataatagt ggtcatgttg actattgtag ttttattaag aatagttggt
    20461 ttatataaat atatgcaaag atagcttgtt gtttacttta cttattagta tataggacaa
    20521 gatactctaa taaatttgcc tattttaata tcttttgtac taaaaccaac taaagtttcc
    20581 tttattattt cgttttgaat aatacaaagc ataatccgat tgtttgtata aagactggaa
    20641 agtatcagag ttattttcta atttagatat acctatagaa caagaattac tctctaggca
    20701 taattctaaa accttctttg ccttattttc aacattctct tttgattgat tactatattc
    20761 aaaaacagca aattcatctc caccaagtct tccaataata gcatcattta cgaatacttt
    20821 agtcaaaata tttgaaaaat gtattagtat ttcatctcct cgaacatgac caaagatatc
    20881 atttacctgt ttaaaattat ctaaatcaaa gatgtaaaag tatccatgtg aaacagtttt
    20941 aagcatttct tttatgtgta cttcaaaaaa atgtctattg tatagtccag tcataaggtc
    21001 tttttctgat tttagtttaa attcatttgc cattgctagt gcttcttgtg ctttttcagt
    21061 tattgtttta gggtaatact catctttcat ttggtcaaag tttggaatag aatggtgaac
    21121 atggataagt ttaatcatat catctgtaac tctgtatacc atagtaaatc gagtatccat
    21181 ttctacatag atatcttttt ctaggtaatc tttttctatt acccaaagag taccatatac
    21241 tataaaagct tcgtctgtaa ctttggtaca tgaataccac tcatctataa tctcaaagtc
    21301 tatttgctct gattctgttt ggtctttttc aagacctata ataaattctt ctaatgtttc
    21361 gtaaaattca tgttttcctg ttccaattaa tgcaaggttg tcatctaata aggaaataat
    21421 aaatttaagg ttttcttctt ctggatttaa aatataaata tgccaaattt tttttgataa
    21481 atctcttaat tgtttttgta ttgtattatt ttccatagat taccccctta agatttacag
    21541 tttatatttt aatgtattat aagttgaact cttattacaa tattattata atatagaact
    21601 aaagaaatta catcctgtat aagtaaaagt atatctttta taataaaaat attacagtaa
    21661 aataaccagt tttaatatac gtattatatt tttaatttat aaacgaatat tataaatgaa
    21721 atttaaattc aaccctgaag aaataagcta aatgtattag tatataaatc catacttgaa
    21781 cataaataac ctttatgata tgatatcaat aataatatta tgagaggagt gccttgatgt
    21841 cggagttata taatggtaac caacaattaa atagttggag acacatatat tctgtataat
    21901 cagaaatttc aagtgtattt ttaatacatt taatttttgt gtcttattct gcctttatag
    21961 ataactgaca tatgacaaca aatagtattt aaaatatata ttactttgtt aaattatggt
    22021 gtagtaggct ttaatctgct acacttttat taattttgta tagtattatt ttgataacta
    22081 ttttgaaata gagagcatta atttttctag tccacaggag ttttctgttt ttgtgctgtt
    22141 aaatataagc acagagaaag gcagatatat acctgtggac tttttttata taaattatga
    22201 aatatggagg tagttaaaat gagtataatt gaacttaatg aactgactaa atcttataga
    22261 agatttgaga aaaaggcagg atttatgggg agtgtaaagt ctttatttaa taggaaatat
    22321 acagaaaaaa cagctatatc gtcatttgat ttaaagatag aagaaggaga atttgttgga
    22381 ttaataggtc aaaatggagc tggcaaaact actcttatta agatgttaac tggtattatt
    22441 gcaccgtctt ctggagaaat atctgtaatg ggttattacc ctaacaaatt agaaaataat
    22501 tttaaaaaac aatatgctgt tgttatgggc caaaaaagtc aactattctt tgaattgaca
    22561 ccagcagata cgtttctttt atttaaagaa ttatatgata tatctaatga agaatataag
    22621 aaaaacttgg attactttat cgaattattt gaggtatctg actatttaaa tgttcaagtt
    22681 aggacgcttt ctttaggaga aagaatgaaa atggaactaa tagtagccct tctacataat
    22741 ccaaaaatat tatttttgga tgaaccaact ataggcttag atgctatagc ccaaaaacaa
    22801 attaggtatt tcctaaaaga agtaaatgaa acaaaaggaa ctactattat acttacttct
    22861 cattacatgg aagatattaa gagtctctgt agtagaagta ttgttgtaga aaatagccgt
    22921 aaaatatatg atggttctac agagaaacta tttaattatt ttcaaaaata taaaaaaata
    22981 acagttagtt tttccaatga aactaacatt gtatttccaa aagattgtaa agttgttgaa
    23041 caaacaaatc ataagatatc agtaattgtg ccaaaagaaa gaggaaatga aacgattaaa
    23101 tggatttttg ataaatatga tgttaaagat gtaatgattg aagaagaaga tataggtagt
    23161 attgtagaga ggatatataa gaaaggaagt gatgagtatt atgagcaact ataatgaagc
    23221 aagttcaaat gattcaatgt tttataaatt taaacagagt attagaaggt attatgaagt
    23281 agccaaaatt aactttaaag tgcaacttat ctggagattt gatgtggtgg ttaatatggt
    23341 gtttacgatt gtaaaaattt tatttgctta tattttatgg aaagcaattt ttggtgaaaa
    23401 taaaatagtc tctggtttta catttaactc tatgttatct tactatataa taagctcatt
    23461 tttagcaggt attgatatgt caaagagtac cagtgaagag atatgtaatc gtgtaaaatt
    23521 gggcaccttt tcaaagtaca tgattattcc aattaatgtt caaaattatt ttattgctaa
    23581 aaattttgga atgacatgtt tttattttgt atttaatatg atttctgcat ttgtatggat
    23641 ttttatattt aaaatagatt ttgcctttac atcaaatcca gttcaaatat taatttcaat
    23701 tttgatgatt tttctagggt ttatttttat gatgaaactc cacttcttta ttgggatttt
    23761 gtcatttaaa tttcttgaga ttagtttttt aaaaatgata attgaaaata ttgttgaatt
    23821 tattacagga acaatgatac cacttgtact cttaccagaa actatagtaa cagttatgaa
    23881 ggtatttcca ttttattata caacatattt gccatctatg ttactaatag gtagaaacag
    23941 caatgaggca atacaaggat taattattct agcattatgg gttttatttt tttctgtagt
    24001 aaataaagtt atgtataaaa gaatgagaac attatttgat ggggtgggta tatgatgaaa
    24061 aaaaatttga aaataatatt tatgttatta aaaatgaaat tatcaaagat gatggtattt
    24121 agattcgatt tttttggagc attttttgta gatagttcat tatttatatt acaacttcta
    24181 atgtttaata gtatatattc tcatgtagac tcaatagggg gatggcaaca aggagagatg
    24241 ttaatattta taggaacttt ctcattaata aatgcgataa atatgacgat tttctttttt
    24301 ggtatttatg atattccacg aaaaatccaa gaaggagaat tagactacta tattacaaaa
    24361 ccattagacc cactatttag gttaacattt gaaaatatta atattggttc atcaccatta
    24421 attctagcaa gtatacttat tgttttatat ggaatatctg agttaaactc agagataact
    24481 ttagttatga taataatata tattttcatg gtgttattga tgacattact tttttatgat
    24541 gtatgtatca tacttagaac agtttcattt tttgttatat catcttctgc gataatgaga
    24601 ttagaagata atttattgcc aatgaatatg aaaattcctg gtgtaatata tgaaggtatt
    24661 tttaaagtat tattctattt aatcttacca tatggaatta tgtcgacaat tccgactcaa
    24721 attttggctg ggacaataac aattaatgga ttaatatact caattttaat tgtatttttg
    24781 tttacattgt ttatgtggag attttggaag ttaggtatga gacattataa aagtgcaagt
    24841 agttaataaa ttagtaaaaa tagacaatgt ataactatat ataaatacgt ttcatatgac
    24901 actaaactat gtgaacaagt atttgcgcta aaggcactga aagctataaa atgattaaag
    24961 taaggattta aaagtttctt tagtagaaat ataattgtgt aaaaaagcaa gattaattaa
    25021 aactatatcc aaataattag atataattct tatgcagaaa ggaggtctgt aaatgtctaa
    25081 ttgtttggaa tatattttaa aaataattaa tgtacagaaa aataaacaaa atgagtgatg
    25141 tatattattc agaaaatcat gaacaagata atctttatga gcaagataat ttactggcac
    25201 aaagatttaa tttatttact ctattgaagt ttgcatttcc tactattttt atgatgattt
    25261 ttatggggtt gtatacaatc gtagatacta tatttgtatc aagatttatt aatacaaatg
    25321 ctctatcagc aattaatatt gtatgtccaa ttattaatct tatagttgga attggaacta
    25381 tgcttgccac tggaggaagt gctattgtag cacgtaagat gggaaatggt gatattgagg
    25441 gagcaaataa agctttttct ttaatcgtat tatcaggagt tgtaattggg gtaataatta
    25501 ctggtatagg tattgtattt atggattata tcattatatg gttaggagca agtgaagttt
    25561 taattaaata ttgtcgttca tatcttttta ttatcttggt ttttgcttct gcaaatatac
    25621 tacaagtatt atttcaatgt ttatttgtaa cagctggaaa gcctcaattt ggattatggc
    25681 tagtgttagg tgctgggtgt gctaatataa ttcttgacta tgtgttcatt gttctgttac
    25741 aaatgggtat tagtggagca gcacttgcaa ctggagtagg atacctaatt cctaccattt
    25801 caggtatggt tttcttttca aaaaataaag ggttacttaa attttgtata cctagactag
    25861 ataaaagaat attatttgaa gcttgtttta atggttcatc tgaaatggtg ggtcaactat
    25921 caacagcagt tactactttt ttatttaatg ctatgatgat gaaattaatt ggtgaagatg
    25981 gggttgcagc tattacaata attatttatt ctcaatttct tttaacttcg ctttatatag
    26041 gtttctcaat gggtgttgca ccaataataa gttataacta tggaagtgaa aataatacgc
    26101 agttaaatca tgtttttaag atttgtatat tatttatttc agtagcatct ttattggtat
    26161 ttatcttttc tatatcatgt tcaccaatca ttgtagagat tttttctcaa agaggaagcc
    26221 gagtttatga aattgcaagg tccggatttt atatattttc ttttagtttt attttttgtg
    26281 gatataatat ttttacctca tctatgttta cagcattatc aaatggaaag gtgtctgcaa
    26341 tattatcttt tctacgtaca tttggattta taactatagg tttattgata ttgccaaata
    26401 tattaaatat aaatgggata tggttagcag taccaagtgc tgagatttta accttatttt
    26461 taactgtttt ttgtatattt aagtataaga atagatataa atatatgtag atattaaata
    26521 aatagcttag atagatattt tatgttttaa taagtaattc aattagtggt agatatatac
    26581 tattgaaaat taaagttata attgatataa agaattaata aaaataacat ataaaagttt
    26641 aaaagggagt tgaacttaaa gtttgattag aaaaagtcaa ttttaagaca actcctttta
    26701 ttttactatt gtattaatct gtatctgtaa gtgtattagt ctaatattat aaactttgga
    26761 tgaaattaat tttctatagg ttttaatgta tcatacactt tattttaaag aaacttatct
    26821 taattttcat agtgattgaa tttgtctatt agagatgtta ctattagtaa tgtaaaagca
    26881 agagtagtga atataaaaaa caagatagtg atactgcaag attagaatat aataaatttt
    26941 tatggagaga tttcaatatg gatacatgtt ttaataaaaa aatattactt gttgatgatg
    27001 aaaaggatat agtagattta attgaagaag tactcataaa tgatggtttt aaaaacatta
    27061 ttaaagctta taatggtcta gatgctattt cgctgtgtaa gatagcatgt ccagatgttg
    27121 tcattcttga tataatgtta ccagatatag atggtataga agtgtgtaaa aaaattcgag
    27181 agttttcata ctgttctatt ctttttttat catcaaaaaa tgatgatatt gacaaaatac
    27241 taggtcttag tagtggggga gatgactata taacaaagcc attcagtcca agagaaattg
    27301 cttttcgagt taaagcgcaa ttacgtagac aacagtatca aagcattgta ccatcagata
    27361 gtgaagttat taaaatagga gatattacta ttgatataga aggaaaccgt gtatataaag
    27421 atagaaatga gattgagtta actggaaggg agtatcatct tttaagctac atggctcaaa
    27481 atgtaaataa aattatagga aaagaaagac tctatgaaca agtatgggga gtatatagta
    27541 gtatttgtga caatacaatt atggtgcata tacgacatat aagagaaaaa attgaagata
    27601 acccctcaaa tcctaaaata cttataacag ttaaaggact aggatataaa cttgtgaata
    27661 gaatcgatta gaatagattt taatattaat gaagatatct caagttgaat ttttagttaa
    27721 tgaggcattc ttttatgggt gaaatcaaat gtattttcgt tatctcaaca atgaagaaga
    27781 ggtctatctc tattttgaga tagcctcatt aagtttatat cctggtaatg tactttatgt
    27841 tctaataaaa tttttttagt ttatttgcag tgaaacatta actagaacat taaaatttgc
    27901 attgaaatac caattaaata atattgtata ttgtgtataa acttatacta attgtttttc
    27961 tatattaaat actttatcaa tccaaccttt ataaaatttt tcttcatggg aaactaggat
    28021 tatacttcct ctaaactgtt tcaaagcatt ttgtaaagca tcctttgtct ctgcatcaaa
    28081 gtgattggtt ggttcatcca gaataagaaa attacaagga gataacatca tacaacataa
    28141 tttaacctta gattgttcac caccacttaa agtagataca ctccttgaaa catgctcttc
    28201 cttcacacca catctggcta gatgacgtct aatctctttt gtatttaatt ttggatattt
    28261 atctgctact atttgaagtg gggttttatc aggattttcc cattttaaat cttgctcata
    28321 ataaccaatt ttaacttgtt ctgaaaactg gaaatctcca gatatacgag gaatatcttt
    28381 gactaatgtc tttaacaagg tagatttacc gattccatta aaaccagtta taactatttt
    28441 ttgacctcca tcaacagaga aatttaattt tggaagtaaa gaatagtagt atccaacttc
    28501 aagatttgtt atagtaagag cattttgagc cgatatttca atttctgaaa attgaatatt
    28561 tggttttcct gtaaaactag gaggagctat tctttctatt ttatccaatt gtttttgtct
    28621 tcctcttgca atttttgaat ttactccagc tttattttta cgtataaatg tttcttcttt
    28681 ttctattttc ttttgttgtg cttgatacct tctaatataa tcttctctta aatgtgattt
    28741 ttgtttaagg aactctgaat attttccatg atatttctta atcatgccaa attctatatc
    28801 aagaatacct gtagaaattt tttctaaaaa gtcaaaatcg tgggaaacta caataaatgc
    28861 cccattaaat gtatttaaat aatttgagag ccattcaaca tgctctttat ctaaaaagtt
    28921 tgttggttcg tctaataata gaacatcatg gttagaaagt agtaattttg ctaaaattac
    28981 ctttgccctt tgaccaccac taagctctct aacaacacga ttcattccta ttgaatctaa
    29041 tccaagacca tttgctattt tatttatttc attatctata gaatagaaat tacttgctat
    29101 tagttgttcc tgataatctg atgctttgat tagatattgc tcattttcag atatagcact
    29161 tttttgatag agtaaattca tttccttttc tattttataa agttcttcaa atgcagtgtg
    29221 taaatagagt gatattgttg tatctctatt gatttctgca tattggtcta aatggcctat
    29281 attgatatta ggttgccact tgatttctcc tgaatctgga acaacttctc ccaataaaat
    29341 tttaattaat gtacttttac ctgtaccatt ttggccaact acacctatat gttcaccttt
    29401 gaataaatca aagttagctt tctcatacag tgatttatcc ataaaacatt gtgataaatt
    29461 agttactttt aataaactca taattcaaac tccttttctt ttattaaaaa atttatggaa
    29521 ataaaaaaag cagaggaatg gcattgtaaa atgccgacct ctgcttttgg aaaaacaaaa
    29581 ggtcatagac aagcattgtt gtctatgacc agaaaatcta tattatctat cggcaaatag
    29641 gtaattttaa ataagcatag ccaaaaaact atactaaacc tatatttgcc gatgaagata
    29701 gaaagcttta agctttcaga ttaacctcta cacaacgctt catcgtatac atttgtgtag
    29761 agttaagttt tctaacaagt ttaactaatt ctatataaat cataattatt cacatcctaa
    29821 tcttagtatt aattctagtt tatctgtatt taaattgtac ttttgtactt tgtggattat
    29881 cttatttatt taaaatatgt tttattaatt atagcttaag ttatgttatc ataatactta
    29941 gttatagtca ataagaaagt tatatttact attattatat taatttagta aatctatcat
    30001 tacaaagtta tttgaagact cgctatcaat aaaattttat agtctataag aaaattaaga
    30061 ttatgagttt tataattttg atatagggat actattttca taaatattac aacatgtaga
    30121 agtatatgtc tattattgtt gaattttcaa tgatatatta aggttctaat atattataca
    30181 aaataaaaac attaattcaa cttaaataat tttaagtatt atatgtgttt tgattaaaga
    30241 aaactaaaat tttaaggaaa aataacatat ttgtattaaa ttttattgaa atgattaaat
    30301 ttatttactt aatgtaaatt attagctaaa attgtattaa ataattaagg aggtgagtag
    30361 ctctggatga aatagattta aaaataatta attctctaaa aaagaatagt agggcttcaa
    30421 cctctgaaat aagcagacaa attaatttat cagtaccagc agtctcagag cgtataagga
    30481 aaattgagca gtcaaattta attcaaaagt acactattca aattaatcgg gaaaaaacaa
    30541 aatataagct tttggctttt atatttatca atactgattt atctaaaagt atgaaagaat
    30601 ttaagaaaac tatattggaa tatgatactg tattagaatg tcatcatatt tcaggagagt
    30661 atgattactt aataaaagta ttagcagaag atacaagtaa tcttgaagag tttatatcag
    30721 tgactttgaa ggaaattggg gggattcaaa aagctaatac aaccattgtt ttatctacaa
    30781 ttaaagaaga atttaataca ataaaagata ttttttaaat aatttactag taaagtgtta
    30841 attatatttt aggaggaaaa tagaatatga aagttttagt ttttttagca aaaggttttg
    30901 aaactatgga atttagtgta tttgtagatg ttatgggatg ggctcgtaac gattatgggc
    30961 atgatattga tgtagtgact tgtggattta aaaaacaagt tatgagcaca tttaatatac
    31021 aagtattagt tgataagacg atagaagaag tatgtgttga tgattatgat gctctagcta
    31081 ttcctggagg atttgaagaa tttggatttt atgatgaagc atatgattca tcttttttaa
    31141 atctaataag ggagtttaat tcaaaagaaa aaataatcgc ttctatttgt gtggcagcat
    31201 tgccagttgg aaaaagtggt gttttaaaaa atcgtaaagc tactacatat catttgaaaa
    31261 atggtaaaag acaaagacag ttaagtgagt ttgatgtaaa tgtagttaat gaaccaattg
    31321 ttgtagataa aaatattata acctcatatt gtccagaaac agctccacat gtagctttta
    31381 agcttttaga aatgttgaca tctaaagaac aaatggatga agttaaatta gcaatgggat
    31441 ttaaactgta atggtttata gattttctaa gtaatcagaa ccccagtcac acatggaatc
    31501 taaaattgga ataatactct ttccaaaatc agatagcgag tattctactt ttggaggaac
    31561 aacaggataa accactctta taattaaacc atctgattct aattcacgta attgttgagt
    31621 taacatctta ggcgttgctt taggaattaa tttttttaat tcattgaacc tcaatatagt
    31681 gtctttaaga tgccaaagta taagtgtctt atatttacca ccaatcaatg caagtgttgc
    31741 ttctattggg caactgtaat tatctaaaca ttttttcatt tttgggctcc tttatttatt
    31801 ttaaagtatc tttttaggta ctatattaca aaaaagtgca ttattgtaat atagtaccat
    31861 agtgataaaa ttatagcata ggttaaataa atcatcaact tagcctaata aaacaatagg
    31921 gggtaaataa atgaatttcg ttgaacttgc aaaaaaaaga tattcttgcc gtaattatca
    31981 agatagaaag gttgaaaaag aaaaacttga gaaggtttta gatgttgcaa gaatagctcc
    32041 aactggtggt aatcgtcaac ctcaaagatt gattgttatt caggaaaaag aaggtataaa
    32101 taaactttct aaggctgcca atatatatga tgcgccatta gcaattcttg tatgtggaga
    32161 taaagacaaa gtttggacaa gaccttttga tgggaaacaa cttacagata ttgacacaag
    32221 tatagttaca gaccatatga tgttacaggc aactgaactt ggattggcaa gtgtttgggt
    32281 gtgctatttt aatccagata taattagaga agagtttagt ttgccagata atctagaacc
    32341 aataaatata ttattgatgg gatatgaatc taagatacca gagagcccag agagacatga
    32401 aaaaactcgt gttcctttaa gtgaaattgt ttcttatgaa actctttaat tataaaatta
    32461 agttattcat aggataaatg taataaagtg tttgttataa acaaaattca aaataaataa
    32521 tatctacata taattaattt tagtgaagta tatctagttt aactagaatg tatacttcac
    32581 taatttattt attcaaaata tttatgagga ttttaatata ttaactaaaa tatatcaatt
    32641 gaattaactt ttaaatctgg aaaagcactt gaaatatagt gttttatatc atttaaatgg
    32701 tcttgaattt gcttttctga tattttatca ggtccatata caaaatacat aatattttta
    32761 gtatcttttg ttatagctgt tcgattatca gggccattaa gaatactaga taatatacca
    32821 atattatctg ataaaaataa atcatctttt gttaaaatat gttctttacc acctatacca
    32881 taaaaatgtt cacttccatt tgctaattct attttcaaag gtaattccat tttatcaagg
    32941 tcatgacctg ctgttagaag aagatttttc aattctgcta aaaacattga ctcaattgtt
    33001 acacctacat ctgggatacc ttttgatttt aatagaatag attctaattg aagcaataca
    33061 gggtaagttt tcttgaattt tttataatac tttatataac ttgaaacagg ctctgtttta
    33121 attttttctt tgcgattgta ttctgggtgt tgttctataa tatttttaat tgtagagttt
    33181 ttaagaacta aaaaattttc tttattaggt tttgaatgtg taatatttat aaccataact
    33241 ccaaatttaa tattgggata aatttcttta actttatgtg atacctctaa cataatatag
    33301 actccttttt attttatatt ttatgatata atcttagcaa attaaaagtg gattttatag
    33361 ttccattttt atagtaattt tatagaacca cttttggagg tattagaatg tggattacga
    33421 ttaatagaaa tagtaatata tcattatcaa gacaaattta ttgtgagata aaagatatga
    33481 ttctggatgg aagcttaaag tttgagcaaa aactgccatc atctaggact ttatcaaaag
    33541 aactatctgt atctcgtaat acagttttag atgcatataa tcaattaatt gcagaaggat
    33601 atttggaaac aaggaatgga tatggaacaa ttgtagctaa aggaatatca gggcataaag
    33661 ttgattttaa gttaaatgat acttttagag atgaaaaaga tttacatact aataaacaaa
    33721 aaaattatat agattttcgt tcaggagttc cagaattaaa actatttcca cgcaaagaac
    33781 tttcaaagct atataaacaa atactgtatg atttatcaga ttcggatttc agatatggaa
    33841 gtacagctgg tgtatgggtt ttaagagtgg aaatatctaa gtatttattt cgtactagag
    33901 gaattagatg taatcccaaa aacattatga ttgtttctgg ttcaacacaa ggtttatctt
    33961 taatttcaca gcttttatac aaaagtaata aaaaggttgc tgtagaagac cctattcata
    34021 aaggcttatt aaaggttatt tcatctattg gatattcagt tgttggaatt aaagtagatg
    34081 ataagggaat gaataccaac ttaataaaat caataaaggg gatttctttt gtttatacta
    34141 caccttctca tcaataccca ttaggaggga ttttacctat tcaaagaaga ctagagctta
    34201 ttaaatgtgc aacagagaat gattactata taattgaaga tgattatgat agtgaattta
    34261 gatatgaggg tcaaccaatt agttctcttt atgaacttag cccaaataaa gtaatttaca
    34321 taggttcttt tagtaaaata ttatctccag ctattcgtgt tggatttatg ttattaccag
    34381 acaatatttt agaaagctat aaaaccttaa aaatgtactc tgatgttcat acagaaacaa
    34441 tattgcaaca tgtattagct gaatttattg gaaatggagg tttagagaag tacatattta
    34501 gaatgaagaa gttgtacaat agaaaacgta agtacctcat aaaagtttta aacacggaat
    34561 ttaaaaatga atttaaaata aaaggtgaag cagcagggct tcatatagtt gctcatttct
    34621 ataatgttaa atttacggaa agtttaattc ataaaatttt tgaagcaggt ataaaagttt
    34681 atcctataca acaatatgca attgagaata tattagaata tgactatgaa attatactag
    34741 gttatgctca tcttagtttt gatgaaattg aaaaaggtgt aaaaatactc agcaaaataa
    34801 tacatatgaa tcaaggtaca tagataaaac atgtagcaat ctacatataa tagatgtaat
    34861 taagtttaaa ctttaaaaga aatatatatt aatatttttg tataaaaata cctgatattt
    34921 ttttatcgat actttattgc tatacactaa attaagatag caatttatat taagaaaaaa
    34981 ctttttgcat aggttataat acttaaaagg agaggataga gttgaaaaca aatacagatg
    35041 ttattatgga gataattgat tatatagaag aaaatcttac agaaaatcta aatttagata
    35101 aaatagctta tattgctaat tattcaaaat accatttaca cagaatgttt acaaatgttg
    35161 taggatgtac tatccatcaa tacatacaga ggaggagact tactgaagca gctagacaac
    35221 ttatatttac agacaaatat attatagaca tagctattac agtaggatat tctacacatc
    35281 aatcttttac actagcattt agaaatttat ataaagaatc tccacaagta tatagaaata
    35341 aagatgaatt ttatcctatt caacagagat ttgttttaaa tctaaatcat caaattcaaa
    35401 gtgaaaaatg tatttcttat gcaaatatga attataaaca aagaagagat gaaatcaatt
    35461 cttatcttaa ctttaatatt aattgtgagg tgaaagtagc atgagtttag tgccacatgt
    35521 aattgaacaa acaggtcaag gggaaaggtc ttatgatata tattcaaaat tattaaaaga
    35581 tagaattata tttataggag aagaaattaa tgatgctatt gcaagtttag tagtagctca
    35641 attactgttt ttagaatcag aagacccaga ttcagatata attatatata taaatagccc
    35701 tggtggctct gctacatcag gatttgctat atatgatact atgaattatg tcagatgtga
    35761 tgtatcgact atttgtattg gtatggctgc aagcatgagt gcttttttac ttgcaggagg
    35821 aacacatgga aaaagatgtg ctttacctaa ttcagagatt atgattcatc agccaatggg
    35881 aggagctaaa ggtcaagcaa cagatgtaaa aatagctgta gataatatat tgaaaataaa
    35941 agaacgatta gataaaattc tttctgaaaa tacaggtaaa tcaatagaag atattaggag
    36001 agatacagat agagataatt ttatgactgc actagaggct aaagagtacg ggctgattga
    36061 ttatataatg aataggaatg aataaagtat caaagtatca tattaaaatg tctgttttac
    36121 aaatatatat ataatattaa ataagagtgt ctcaaaatga actttttagt ttatgggaca
    36181 ctcttttttg tatgaaatta aatatggttt tatcatttca acaattatta ccaaatttca
    36241 agtttaataa attctactat aaaaaataca taaaagataa ggttctttag taactttctg
    36301 tcgtcaaaag tttttataaa tttaataaca aaacaagaga aaataccata tattacttat
    36361 gtctgcaata tatagaagac aaaggagttg tacattatga ttacaataag tttgtgcatg
    36421 attgttaaga atgaggaaga agttataggc agatgcttag aatgtgtaaa ggatatagta
    36481 gacgaaatta taattgtaga tacaggttct acagattcca caaaagaaat agtctcaaaa
    36541 tatacagaca tggtgtacga tttcgaatgg atagatgatt tctctgctgc aagaaatttt
    36601 tcattttcta aagcttctaa agactatact atgtggcttg atgcagatga cataatttta
    36661 gaagctgata gagaaaaatt attaaagatt aaagaaagct tggatacttc cattgatatt
    36721 gttatggcaa aatacaatgt atcctttgat gaaaatggta acccaactct ctcgtattat
    36781 agagaacgat tgtttaaaag aagttctaat cataaatggg ttgaccctat tcatgaagta
    36841 gtacctcttt ttggagaggt attttattct gacattgcaa tatctcataa aaagcttcat
    36901 agacaggacc ctctaagaaa tcttagaatt tttgaaaaaa tgatttcaga aggaaaaaca
    36961 ttggagccaa gacaccaatt ctattattct agagagttgt actataatgc taggtataaa
    37021 gaagcaatag aaggttttac taagttttta gactcatcaa gaggatggat tgaggattgt
    37081 atcagtgctt gcagagattt agccacatgt tattacttaa ttaatgatga aaaaagtgcc
    37141 ttatattcat tgtttagaag tttcgaattt gatgagccaa gagcagaaat ttgttgtgac
    37201 ataggaaagc atatgtttga tagacaaaaa tataaagaag ccattttttg gtataaggta
    37261 gcattaacaa gagataagaa tgatactaat ggaggtttta agtcaaatga ttgttatgga
    37321 tatataccat ctatacaatt aagtgtctgt tatgacaggt taggagaatc agataaagct
    37381 atttattatc atgaaaaaac aaaagaaatc aaacctaacg atagtgctgt tttgcacaat
    37441 gaaaattatt ttagtaaatt taaaaatagt tgataggtca caatcatact aaatttgaca
    37501 tattatatat agagcttaaa agctcaaata tatcaggagg tgcttttaat aatgagtaga
    37561 aataaatatt ttggaccatt tgatgataat gattacaaca atggctatga taaatatgat
    37621 gattgtaaca atggtcgtga tgattataat agctgtgatt gccatcattg ctgtccacca
    37681 tcatgtgtag gtccaacagg cccaatgggt ccaagaggta gaaccggccc aacaggtcca
    37741 acgggtccaa caggtccagg agtaggggca acaggcccaa caggaccaac cggtccgact
    37801 ggtccaacag gaaatacagg gaatacagga gcaacaggat taagaggtcc aacaggagca
    37861 acaggggcaa caggcccaac aggagcgaca ggagctatag ggtttggagt aacaggtcca
    37921 acaggcccaa caggagcgac aggagcaaca ggagcagatg gagtaacagg tccaacaggt
    37981 ccaacgggag caacaggagc agatggaata acaggtccaa caggagcaac aggggcaaca
    38041 ggatttggag taacaggtcc aacaggccca acaggagcaa caggagtagg agtaacagga
    38101 gcaacaggat taataggtcc aacaggagcg acaggaacac ctggagcaac aggtccaaca
    38161 ggggcaatag gagcaacagg aataggaata acaggtccaa caggagcaac aggagcaaca
    38221 ggggcagatg gagcaacagg agtaacaggc ccaacaggcc caacaggggc aacaggagca
    38281 gatggagtaa caggcccaac aggagcaaca ggagcaacag gaataggaat aacaggccca
    38341 acaggtccaa caggagcaac aggaataggg ataacagggg caacgggatt aataggtcca
    38401 acaggggcaa caggaacacc tggagcaaca ggtccaacag gagcaacagg cccaacagga
//
LOCUS       DAMJWS010000032        37266 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMJWS010000032 DAMJWS010000000
VERSION     DAMJWS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.032"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            69..1073
                     /locus_tag="PC399_001725"
     CDS             69..1073
                     /locus_tag="PC399_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2592587.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVNQDMVLFFQKKISKVSR
                     DNDIRTAVFNNYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDKYHLRVLDKDAEKEEANSKFVTEFLNATKIKDDKYKTKKFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLREKHEIDINDFVDKTIKDDKLKDSFKEHMEEKGLVEGFSI
                     DKKWVDKKLKKRNIKTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIVIKNVTFYEEK
                     "
     gene            1155..1484
                     /locus_tag="PC399_001726"
     CDS             1155..1484
                     /locus_tag="PC399_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592588.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNDTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            1521..1892
                     /locus_tag="PC399_001727"
     CDS             1521..1892
                     /locus_tag="PC399_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592589.1"
                     /translation="MFNIYKVKIKTKRTLEQVRNQSVDFEYSEKGLKNTLKYYNLIDD
                     LKVIVVKFGDEYCLANYNEEDRKIIMEAHYLLEQDEYTGCYINEYERFKKDWENGNCD
                     GEACMVFSDDEIEIIEKLREG"
     gene            1895..2077
                     /locus_tag="PC399_001728"
     CDS             1895..2077
                     /locus_tag="PC399_001728"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592590.1"
                     /translation="MNKRIKMKKRLIHKKCDERCVNYDFVISNNLITCNVCIGCKYKE
                     NMDKVCEENYKKLRSK"
     gene            2157..2498
                     /locus_tag="PC399_001729"
     CDS             2157..2498
                     /locus_tag="PC399_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HDF2592591.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNFKECESYLEGKNELYE
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELTK
                     INSHLELEDLR"
     gene            2624..3031
                     /locus_tag="PC399_001730"
     CDS             2624..3031
                     /locus_tag="PC399_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2592592.1"
                     /translation="MKVFLVIDGEPVGKERPRMNTYAKRTYTPNKTKNYEDLIKWLYQ
                     SKVKYYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVIKVVADSLN
                     EIAYKDDTQIVEVVASKYYSDNPRVEVILEDVI"
     gene            3133..3981
                     /locus_tag="PC399_001731"
     CDS             3133..3981
                     /locus_tag="PC399_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDF2592593.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHNIEGGFGEHKKAMLVKDIAEI
                     HNRESRQINELINKNRKRFKDGKDILDLLGVGLDDTKIKELGFTQQSINSYRGLKNKG
                     LLSGIYILSERGYAKLLKILEDDIAWELYEKLVDGYFSMRKELNNPLLSASKELQAIF
                     MLDKKQEVLETKIENVNEKLENFMDDAPLFNIECESIVKEVKKVATKSLGGHGSKAYK
                     NKSLRGKVYNDIYHQIKREFGVDSYKAIKRCQLNKVLEIVNNYKLPIVFEEEIRLLNS
                     QLSIVS"
     gene            4084..4572
                     /locus_tag="PC399_001732"
     CDS             4084..4572
                     /locus_tag="PC399_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592594.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSTVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKVMDMLYPRIKENELPLF
                     KN"
     gene            5319..5492
                     /locus_tag="PC399_001733"
     CDS             5319..5492
                     /locus_tag="PC399_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592595.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFGRF"
     gene            5492..6103
                     /locus_tag="PC399_001734"
     CDS             5492..6103
                     /locus_tag="PC399_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2592596.1"
                     /translation="MSGYLIVNLSNMLGELEEEEVKKILSSFSCPLNKDVEEFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIVGYFTLANKYFTIKRKTLSNSLARKIVKFGQYNE
                     ELRRYIIGAPLIGQIGKNYSNNYNKLIKGDELLKIACDKIKAVQLDMGGKIVYLECED
                     KPKLIEFYKDNGFVDFGKRSLDKDETDSLDGDYLVQMLKYLKK"
     gene            6180..6368
                     /locus_tag="PC399_001735"
     CDS             6180..6368
                     /locus_tag="PC399_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592597.1"
                     /translation="MEIKKNTQDVVSERKNSLDSEFKIPASGVCYMAEFIKESREIIK
                     ELDKHFESCLDVLSKARL"
     gene            complement(6348..6665)
                     /locus_tag="PC399_001736"
     CDS             complement(6348..6665)
                     /locus_tag="PC399_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592598.1"
                     /translation="MSKNIDIRNLKQFQKKIERMEQNLKPDFDKIRTIILSKKFMNQH
                     TNFDSFDELLAFGNYIVNSEEDFLAIPDNEFDLFIVKNTDFPDWQTMLDSAYSKYLES
                     CLR"
     gene            6789..7016
                     /locus_tag="PC399_001737"
     CDS             6789..7016
                     /locus_tag="PC399_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592599.1"
                     /translation="MGFEISGNLNFDSVIDDLKKEVENNPTIFTSQNVGNKFKEKCKI
                     CEKISEFEILEDGKVKCLECGTEFELNLKVE"
     gene            7086..7778
                     /locus_tag="PC399_001738"
     CDS             7086..7778
                     /locus_tag="PC399_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2592600.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            7756..9009
                     /locus_tag="PC399_001739"
     CDS             7756..9009
                     /locus_tag="PC399_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2592601.1"
                     /translation="MVIEMIVRIDFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFTTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSIGLEKTLFMYCDSAEPDRIKMWKNAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            9015..10454
                     /locus_tag="PC399_001740"
     CDS             9015..10454
                     /locus_tag="PC399_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2592602.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFVERGNSFVQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFISFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            10444..11841
                     /locus_tag="PC399_001741"
     CDS             10444..11841
                     /locus_tag="PC399_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2592603.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAMNSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIRHGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRHIYTSGYYHAAFNVQQGVNVGWS
                     LMNLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKNLV
                     NDFSKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLKTSNICRE
                     LDGKVFDMKDYQVGVTAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSSMKYN
                     EWYEKYVKHNSILEIKNSAIIDSIKEDIKNGKYNLNIHDGKQGKHLKEHNNYIEGRSY
                     LTITKEEAQELVNKHAGNGIIKFNRSGEWDKKELIEVDKNIGVNVNNITGEKTLTNKF
                     KIHYSKTGTHIVPAL"
     gene            11856..12056
                     /locus_tag="PC399_001742"
     CDS             11856..12056
                     /locus_tag="PC399_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592604.1"
                     /translation="MKLWEYVGKNVQITCVDKQIIRGKCDGYTQALDNEPEIASISIA
                     RDGYGIEVYENEIESIENINKE"
     gene            12122..12685
                     /locus_tag="PC399_001743"
     CDS             12122..12685
                     /locus_tag="PC399_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2592605.1"
                     /translation="MDWLKELLEGIKIEDNKIDVASLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            12697..13596
                     /locus_tag="PC399_001744"
     CDS             12697..13596
                     /locus_tag="PC399_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2592606.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            13596..13829
                     /locus_tag="PC399_001745"
     CDS             13596..13829
                     /locus_tag="PC399_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592607.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKVVRYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            13839..14240
                     /locus_tag="PC399_001746"
     CDS             13839..14240
                     /locus_tag="PC399_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592608.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEADMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTAYGKNKILTFRCLRW"
     gene            14234..14581
                     /locus_tag="PC399_001747"
     CDS             14234..14581
                     /locus_tag="PC399_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592609.1"
                     /translation="MVSKTRKAIEMLYRYKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            14581..15006
                     /locus_tag="PC399_001748"
     CDS             14581..15006
                     /locus_tag="PC399_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2592610.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            14999..15436
                     /locus_tag="PC399_001749"
     CDS             14999..15436
                     /locus_tag="PC399_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592611.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEAKNFEINEIAEMLLEELEYIEINGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNNADKMNTVELFGGLKRGDNFE"
     gene            15429..15605
                     /locus_tag="PC399_001750"
     CDS             15429..15605
                     /locus_tag="PC399_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592612.1"
                     /translation="MSKTLSKGTDYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            15606..16916
                     /locus_tag="PC399_001751"
     CDS             15606..16916
                     /locus_tag="PC399_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2592613.1"
                     /translation="MALGGGTFVTQNKVLPGSYINFVSATRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CSIATARCSGIRGNDLKVIVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITELQDNDYV
                     TWKKEATLEATAGLTFTNGTNGEAVTGTEYQAFLDKIESYSFNALGCLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKIKDTGLLESSLIYWTTGAIAGC
                     DINKSNTNKRYDGEFDVDVNYTQIHLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     EKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            16938..17408
                     /locus_tag="PC399_001752"
     CDS             16938..17408
                     /locus_tag="PC399_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2592614.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            17461..17718
                     /locus_tag="PC399_001753"
     CDS             17461..17718
                     /locus_tag="PC399_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592615.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            17723..18163
                     /locus_tag="PC399_001754"
     CDS             17723..18163
                     /locus_tag="PC399_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2592616.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPVLNKKGKATGQYTSETDFNTYTLKLCVETVVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            18484..18645
                     /locus_tag="PC399_001755"
     CDS             18484..18645
                     /locus_tag="PC399_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592617.1"
                     /translation="MDNFLLSILASLIASLIGYIVCRCIKNVKSHSTRGKSKSGWELG
                     FKIKFRKFK"
     gene            18794..19027
                     /locus_tag="PC399_001756"
     CDS             18794..19027
                     /locus_tag="PC399_001756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592618.1"
                     /translation="MNKDIELTEKDLYCIARHIQVYVFKKDDEVIKKEDNPCCRCKHK
                     FKYYENSVCIYHCSVFQKLSKITGLKMGFGVKL"
     gene            19087..19614
                     /locus_tag="PC399_001757"
     CDS             19087..19614
                     /locus_tag="PC399_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDF2592619.1"
                     /translation="MREGKRRRGCLFWFIIIIIFSGVVGAIAGNSTNNGSTEKQKEDL
                     TKYIGEEGNIGDLKLTVNSISKASEIPVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDNSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            19756..20316
                     /locus_tag="PC399_001758"
     CDS             19756..20316
                     /locus_tag="PC399_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2592620.1"
                     /translation="MFCSNCGAEITGIGKFCSSCGVAVETEIIEDNNIKSNDLIVDAN
                     GIEINMTEIYRKYRKEKVNAIKNVMEISGLNIKEAKKIVDFSFEELKINFIDDTMSNS
                     EKEKIIQNQNRKNNIEKAQQESVACCPKCGSTSLTAQKKGFGIGKAMVGASLTGGIGL
                     VAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            20394..22682
                     /locus_tag="PC399_001759"
     CDS             20394..22682
                     /locus_tag="PC399_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2592621.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTTAMNIVLSSFEQLQRTSSNAVN
                     ANSIIRAREELARAEAGFDRLERQIRESDNQQRKLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLGMINDGQQSDAGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLSSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTFSQIWTSIKNNAIMIFGVIQKKIEQSMSSKGFRTFIDNFINSLYVLGNVAYNI
                     FNEIISILGSPFFQAFVNAIIVGVSLIVQALGWIITQALNIANVFAQNWSIIAPIVLG
                     VAAAMLVYNNALLLSIANKVKDIALSAKSLVMSFAHIVAESYRAAALVATTIAQDGLN
                     AAMAACPITWILYGIIAIVVAFFVAIAIFNHFAGTSVSAIGVVAGAISVAASFIGNLF
                     IATGNLIIDIVALIYNTLAGFAEFFANFLDDPIGSVIRAVSGMANAVLGIIRSIASAF
                     DTVFGSNLADAVSGWQDKLQGWTDKVAGEAKIKVERMDPNKLHFDRFNYGKAWDAGYK
                     WGDKLETNIKDKFDISKMAEDAKKKLGLDDLWDKKYGLGDGFGSAGLNSPLNDAAKGA
                     KDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVNIKIDMNNTNNISKDNDLD
                     GIVNVLTEKLNDAMAVSAEGIV"
     gene            22699..23400
                     /locus_tag="PC399_001760"
     CDS             22699..23400
                     /locus_tag="PC399_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2592622.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWAICQKQLGNGSLYKKVYELNKTMMDKANKGKK
                     LSKYTIYKGQVLRLV"
     gene            23393..25363
                     /locus_tag="PC399_001761"
     CDS             23393..25363
                     /locus_tag="PC399_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2592623.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFKLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLSLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKNEKQNGPAQSTTSKEDTDMANKINKLLKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKKNGDYMSFSSVDEGIKRGISNLSRNY
                     IHIGRKTLESIRNKYSSSSDKEWVKCVGAFYKQITGSTYNSNSAGTGVGSNEEAEKNL
                     KDLTYQVQNNNSNTSTNNNNKVSKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAFK
                     RGAGINLKRVSADQSKDSRGKLLCNINDVKAGDLVFFAYNKGKGNVHHVGLYIGNDQY
                     IHAPQTGDVVKISSLSGRQKKKHDFARARRFF"
     gene            25377..25637
                     /locus_tag="PC399_001762"
     CDS             25377..25637
                     /locus_tag="PC399_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2592624.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPMQIAFGTIESVNPLIVKIEQKA
                     SFEEFFLIQTETFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            25642..26061
                     /locus_tag="PC399_001763"
     CDS             25642..26061
                     /locus_tag="PC399_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2592625.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            26062..27111
                     /locus_tag="PC399_001764"
     CDS             26062..27111
                     /locus_tag="PC399_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2592626.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEGIYKMKCETAGPIFELGQLIPIEYIDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TINSNSIVVRGEVVG"
     gene            27104..27721
                     /locus_tag="PC399_001765"
     CDS             27104..27721
                     /locus_tag="PC399_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2592627.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVGNKEIKILHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            27733..28758
                     /locus_tag="PC399_001766"
     CDS             27733..28758
                     /locus_tag="PC399_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2592628.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTNNLETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWVEDAENGFKATVTHSLLTQRIVVNIIDVTTKENVVTNFKIIDDNSIEIRSEVKA
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRLINIEEKVNGGLSSIATSV
                     NELITYC"
     gene            28773..30455
                     /locus_tag="PC399_001767"
     CDS             28773..30455
                     /locus_tag="PC399_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592629.1"
                     /translation="MQTEWNFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWYTDAK
                     GGYSKGEITLKKQTTLYVYVGESGFASSSTSNNTKSGFNGGGKGYLNQQIMGTYYSMY
                     GGGATDIRLVGGAWDNEQGLLSRIIVAGGGGGSYYPSTGGAGGGLEGGTGYSSNDRYR
                     PGGTQYQGGIGRVNTENGSFGKGCSVKDSTGEGGGGGWFGGAGMNGVGAGGGGSSYVL
                     TKDSYKPTGYTPTSEYYFDNIVMTPGGNTAGAYGYAQITLLQSLPFLNISSYNSTQAT
                     FKADHTDPTLLTKIEYFIDDVLKETITTDLTEEKTINYTLEDNALHTLKIVVTDSNNA
                     TAEKAVSISKNIMPLPENVNLNDISTKLVEVNAGFKTGKTSIINTLALKNIEASLNNT
                     LVELSEKIKTSFDSSDASVQDLMNQLTQANNTISQLNSKYKVASGNSVVNYCTNTKGF
                     YFNSDYLFLFPGAIQINGLNFVPNIFFTTFELIDDGYFHKYFVFACRGIFTQDFVITA
                     HYYRLTSYLQDFKVGGEVLKLNERDVYMDNRGIQLPCSRQGSSFKWQAIKFI"
     gene            30472..30765
                     /locus_tag="PC399_001768"
     CDS             30472..30765
                     /locus_tag="PC399_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592630.1"
                     /translation="MDRANRIIYDQTGKILLQTGEATGDILEHDTITELHYIDVEYGS
                     IDYTRNRIIGINIETKEPILEEIPVFISEEEKRIQELENQILLNENKKVGGIL"
     gene            30765..30947
                     /locus_tag="PC399_001769"
     CDS             30765..30947
                     /locus_tag="PC399_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592631.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNAEYRKAVED
                     YIIRESGVVEEAEPTI"
     gene            30980..32211
                     /locus_tag="PC399_001770"
                     /pseudo
     CDS             30980..32211
                     /locus_tag="PC399_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32208..32507
                     /locus_tag="PC399_001771"
     CDS             32208..32507
                     /locus_tag="PC399_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592632.1"
                     /translation="MIYLGNFMETDEQNIKYIGMIHYKPTLLSEEELKNGILIEKLPV
                     QQYVENKEAKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            32509..32646
                     /locus_tag="PC399_001772"
     CDS             32509..32646
                     /locus_tag="PC399_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDF2592633.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVTKNKITASEYKEIVREDYI
                     A"
     gene            32708..33139
                     /locus_tag="PC399_001773"
     CDS             32708..33139
                     /locus_tag="PC399_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2592634.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGSK"
     gene            33139..33946
                     /locus_tag="PC399_001774"
                     /pseudo
     CDS             33139..33946
                     /locus_tag="PC399_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            34027..34389
                     /locus_tag="PC399_001775"
     CDS             34027..34389
                     /locus_tag="PC399_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592635.1"
                     /translation="MEDLCMDALVYERFVRAAFSIKLNNLINRSEDLGGLAEADIFRA
                     ANNLHELNEIKIGTGYAIAIFNNEILESCNVSDNDSNRMIELFDRSLIATSREEILDI
                     IREYETYRGRYLTFNWKR"
     gene            34555..35073
                     /locus_tag="PC399_001776"
     CDS             34555..35073
                     /locus_tag="PC399_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592636.1"
                     /translation="MDKKIDIAKFIVNRIDYYIEKSDNKASFLLLLNSAIIGFLFSGK
                     EKIMNHLSISNIRCLEMLFYIVILCIFVISIYFSIMVLKPRNSKIENEYKSIFYYKEI
                     ASLNNEQYKEAFENAFKDEENLINDALVQIKELSLICDKKMYNVKKSVEAFILGGIIL
                     FIYILVVVFNAI"
     gene            complement(35137..36723)
                     /locus_tag="PC399_001777"
     CDS             complement(35137..36723)
                     /locus_tag="PC399_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592637.1"
                     /translation="MVRIFTMVDDKEYKTQNVNNVHNSAKNENAFNLKKYKEKLQENI
                     NTDIYEKEKEPNHPEVILFFSFDIANSSLYKNINYSGWAKVLSHIIRKLQYRVYENLK
                     AQLWRVLGDEVIFIIVLKNYDEIYKYIDIIFDILTSTAKDIKSGNIFSTLEGFSESEK
                     YLMKLQNIISLKGAAWIAIVSRNPNFNALENNEQYENISAMYDLSNNYKIFEFLGNDI
                     DAGFRISKQTCPERLVLSFELAYILSRKTDILSKLHIITYKKLKGIWKDKLYPIIWYH
                     NKGKNNDIEFDDSFSFDEIEENELVREYFFNKKGESKLLIDSFMFNSVDKALDKILID
                     RNLSDKIEKIGDVISKTNPSYDKNTIDKDYIKVDLMELHCVAVCYNKSTSKILIAKRS
                     DNRNNNASKWEFGCAKASLETSIINTIKDEYEKDFNINIEPITDCTRKDDCQPIPLAI
                     YQVKKSDGLHKGIITLAEIINDYDISKFEPTSKHNELAWIGEDELEDFNENTVPDFKE
                     TLKLAFKKLNENQLQESTNM"
     gene            complement(36699..37220)
                     /locus_tag="PC399_001778"
     CDS             complement(36699..37220)
                     /locus_tag="PC399_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF2592638.1"
                     /translation="MDFKIRELINDITQDIIQTYKIQIPIVNINQVVDALGGKVIEDS
                     SLSGYSDGFIRKVDDSFEIVVSPYQPDTRKNFTIAHELGHLFLHMGYGIDDELWNSQD
                     GNQYFRSGNTNKEYQSNEFAAALLMPKHEYKRIMDENTVGNKVDTSKIAEYFNVSSYA
                     ASNRGKWLGYLQW"
BASE COUNT        14947 a         4000 c         6924 g        11395 t
ORIGIN      
        1 gaagttataa aaaatactat aagcgagaag attatagaac gttttgaaaa tttataaggg
       61 gtggaattat gataatacac aaatttataa tacatgtttt agataagaat agtgatacac
      121 caatattgaa tgattttgaa ggtagggtta atcaagatat ggtcctattt tttcaaaaga
      181 aaataagcaa agtatcaaga gataatgaca tcagaacagc agtatttaat aactatagta
      241 acaatctaat taagaagtgt tgtgaacaaa ttatttatga tgaaagttca tttttaaata
      301 actctaaaga gattgcagct tatttatttg atgttatgaa attgaatgct acattagaat
      361 cttgcgactt agcaatttgt ttatactctc aaaaagatga aaagaaagtt gctatattaa
      421 agcttgatta caataattcg tatactcatt caattgagtt taaagatgat aaatttaata
      481 tacagatgtc taaaaatgaa attaatatac aagagactaa gacggttaaa attgctgctt
      541 tggttggatt gagtggaatg aatgacaaat atcatcttag ggttttagac aaggatgcag
      601 agaaggaaga agctaattct aagtttgtta cagagttctt aaatgccact aagataaaag
      661 atgataagta taagactaag aagttcaaaa atacagctga gaattggata actaatgctc
      721 ttagtaatga tataaaacaa gcagaggatg taagaagtat attaaattat actttgagag
      781 aaaagcatga aattgatata aatgattttg ttgataaaac aattaaagat gataagttaa
      841 aagatagttt taaagaacat atggaagaaa aaggtcttgt tgaaggattt agtatagata
      901 aaaaatgggt tgataaaaag cttaaaaaga gaaatataaa aactgacaat ggctttgaaa
      961 taaaaggtaa cttaactgat tttgaggacc caatgaaata tacagtaaga caaaatcaaa
     1021 atgggtctat agatatagtt attaagaatg taacatttta tgaggaaaag taggtactca
     1081 tgtgactatt ggctagagaa ggagaagtaa ataataagag gatgtaaatt taaactagtt
     1141 aggaggaata acttatgaag atttttttat tgactatact gctaataatt atttgtatat
     1201 tagcaaatta tgtgaaaaat cgcatatata aaaaatctat aaataatcta aaatataaat
     1261 attctgtagg ggaaaagatt atatatcatc aaataaactg ttactataac agaatggttg
     1321 gttgtgaaat tttagaaaaa tgttatagta cgaaatttag aaaaagaaat accccgcttt
     1381 ataaagtaaa agcatatgta ggtgataacg atacaacatg ggttatacca gagtggagaa
     1441 ttgaatgtct tgctacgact tatggagaat ttcctaaata ttaaataata aaaattggct
     1501 ggagaaggag attgtaaatt atgtttaaca tctataaagt gaaaataaag actaaaagaa
     1561 cattggagca ggtaagaaat caaagcgtag actttgagta ttcagaaaaa ggattaaaaa
     1621 atactctgaa atactataac ttgattgatg atttaaaagt aatagtagtt aaatttggag
     1681 atgaatattg tctagctaat tacaatgaag aagatagaaa aataataatg gaagcacatt
     1741 atcttttaga gcaggatgaa tatactggat gttatataaa tgaatatgaa cgatttaaaa
     1801 aagattggga aaatggtaat tgtgatgggg aagcctgtat ggtattttca gatgatgaaa
     1861 ttgagataat tgagaagcta agggagggtt aaatatgaat aaaagaatta aaatgaaaaa
     1921 aagattaatt cataaaaagt gtgatgaaag atgtgtcaac tatgactttg taattagcaa
     1981 taaccttata acttgtaatg tgtgtatagg atgcaaatac aaagaaaata tggataaagt
     2041 atgtgaagag aactataaga aattaagaag taaatagaat aaaatagtca aggtaagttt
     2101 gtgaatgaaa ctagaatgtt atagacttac tttgacttat aaaaggagtg tgttaaatgg
     2161 ctaatatata ttgtgaaaat tataattgta aaaactactt tgaagatatg tgtatgcttg
     2221 aaagaattga aattaataac ttcaaagaat gcgaaagcta tcttgaaggt aaaaatgagc
     2281 tatatgaatt agaaaatgga tatactatac atcctaaaga tttgaaaatg gtgaaaagta
     2341 aagattattc tgttgaagtt actcatattc caactggtat tacagtaaaa tgccgttcta
     2401 caaatagtat tttaaaaaat aaaaataagt gtttggaagt tctagaagaa gaactaacaa
     2461 aaataaactc tcacttagag ctagaagatt tacgctaaat aggaagtgag cttatgaaac
     2521 gaagaagatg cagttggtgt ggtaagttat tttatcttga agaaaaatct aaggatgttt
     2581 attgttgtaa agaatgtagg aagaaggcta agaaggtgaa aaaatgaaag tttttcttgt
     2641 aatagatgga gagccagttg gcaaggaaag accaagaatg aatacttacg ctaaaaggac
     2701 ctatacacct aataagacta agaattatga ggacctaata aaatggctat atcaatctaa
     2761 agttaaatat tactttgaag gttatataaa aatgacttta agatgttatt attctatagc
     2821 taaaagtaac agtaaaaagg ttaaggagca gaaaagaaat aatgtgttaa gacctagtaa
     2881 aaagcctgac attgataatg tcattaaagt agtagctgat tcactcaatg agatagctta
     2941 taaggatgat acacagattg ttgaggttgt agctagtaaa tattatagcg ataatcccag
     3001 ggttgaggtt atattagaag atgttatcta accaacggaa aaatccgttc gttaaattat
     3061 gcccttagtt tttcataaaa atgtgaaaaa gctaaataaa gaatatatca aacgacaaag
     3121 gagagataaa ttatgaatga aaatataaat aaagaaataa cagtacttgg aactttagaa
     3181 atcgagggaa tgaaatttca taacattgag ggtggatttg gagaacataa gaaagcaatg
     3241 ctagtaaaag atatagccga gatacataat agagaatcta gacagattaa tgagcttata
     3301 aataagaata gaaaaagatt taaagatgga aaagatatat tagatttgtt aggtgtcggt
     3361 ttggacgata ccaaaataaa agaattagga tttactcaac aatcaattaa ttcttataga
     3421 gggttaaaaa acaaagggtt attatctggg atttatatat tatctgaaag aggttatgca
     3481 aaattattaa aaatattaga agatgatata gcttgggaat tatatgagaa gttagttgat
     3541 ggatatttct ctatgagaaa agaactaaat aatcctcttt taagtgcatc aaaggagtta
     3601 caggctatat ttatgctaga taagaaacaa gaagtcttag aaactaaaat agagaatgtt
     3661 aatgagaaat tagagaactt tatggatgat gcaccactat tcaatattga atgtgaaagt
     3721 attgtaaaag aagttaagaa ggtagcaaca aaatcactag gaggtcatgg aagtaaggct
     3781 tataaaaata aatctttaag aggtaaagta tacaatgata tataccatca gattaaacga
     3841 gagtttggag tagatagtta taaggctata aagcgttgtc aattaaataa agtattagag
     3901 attgtaaaca attataagtt acctatagtg tttgaagaag aaataagact tttaaatagt
     3961 caattatcaa tagtaagtta aatttattca aaaaaaagga aaaggagtgc tttcacactc
     4021 cacttgtcaa aaatataaaa cttttatcca agattattat aacataaaca ggagtgtgga
     4081 agtatggata ataatatcaa taaaaaagaa ctatttaaaa aagtagaagg tagattacat
     4141 cattataaat ttttaagtgc agaaattaag aatcttgaat tagatataga aagtagagaa
     4201 aatgagatat ttgggtgtaa ggctgttgga tatgatgaaa aagtaagtcc aacatatgct
     4261 tttaattcaa ctgttgagaa tgagattata aaaaaagaaa gagatattac tagattgaaa
     4321 aaactgaaaa aagataaaga aatagaaaag aagaaaatag aaaatgcact tacatgctta
     4381 gatataagag aagaacattt ttttaaactg ttttataata gtagaatgaa aaatagtatg
     4441 gtttatatat ccttagagat gaactcagat aggaaaacat gtagatgtgt aagggaaaga
     4501 ttagtgtata aagttatgga tatgctttat ccaagaatta aggaaaatga actcccatta
     4561 tttaaaaatt agaaaattcc ccagtttttc cccagaaatt cccactttat tccctacttt
     4621 ctcccctttt tgattaaaaa agcatgagat aatagtattg tggaaataaa gatttccctc
     4681 tcaaaactta atatttgact agggtataag ggattgccct agtcactacg aacagactag
     4741 gcagggcgtg aggacgctgt tagttcaatt ctaactatgt tcaaatatta atcaacgtat
     4801 acactaaaag tagagaaatt gagggcaaaa ttttatattt tgtatcttaa ttcagaagtc
     4861 taaaaatcgg gtggggcttg gtaacctcac tcaccatgca ggtactggtg tctagtctaa
     4921 gttcgattct tagaacctgc gacataatat atgtatctcc ctactaaaaa tgctaagttt
     4981 actccaaact tagcatttta atttttaaaa agaaaaaaga aatttttatt gtcataatac
     5041 tatttgttta ggtatattat aatgtgcata cttaaaatta aatacttagc aagcatttga
     5101 attaatatac ataagatata tgacataatt ttttatagtg taagttattt aaattaatta
     5161 taaataacag taattttatt atataaaatg tacatattgt gaataataat aataaaatca
     5221 tgtacaaaat gccaactgat aattcctcga aatatattgc atatttaacg taacgtcagt
     5281 ataattaaat tataatagtg aagtggaagg tggtacttat ggctacaaaa agtattttaa
     5341 aaaatgtaga tgtaagaaaa aaggcatttg gaagaaatct agtatcagct ctagaaaatg
     5401 ctaaaaataa acaagaaaaa gaagttgtat taagtaaaaa atgttcagaa gtaccaaaag
     5461 ataaaataaa ggatatattc gggagatttt aatgagtggc tatttaattg taaacttaag
     5521 taatatgcta ggagagctgg aggaagaaga agttaaaaaa attctctcca gtttttcttg
     5581 tccccttaat aaagatgtag aagaattttt aaaaaacaaa gctattgaat tttctaaaca
     5641 gggtttggct agtacacatt tagtgctaac ttcttataaa ggcaagcctg ttatagttgg
     5701 atatttcact ctagctaata agtattttac aataaaaaga aaaacattat caaactcttt
     5761 agctaggaaa atagtgaagt ttggacaata caatgaagaa ctaagaagat atattattgg
     5821 agcacctttg atagggcaaa taggaaagaa ttattcaaat aattataata aattaatcaa
     5881 gggtgatgaa cttctaaaaa ttgcatgtga caagataaaa gcagtacagt tagatatggg
     5941 tgggaaaata gtgtaccttg aatgtgaaga taaacctaaa ttaattgaat tttataagga
     6001 taatggattt gtagactttg gaaaaagaag ccttgataaa gacgaaacag attcgttaga
     6061 tggggactat ttagttcaaa tgttgaaata tctaaaaaaa taaaagtaca taaaatctaa
     6121 aatgactatc ttgatagatg gtcttttttt atacaataaa ttaaaaggag aatgaaatta
     6181 tggagattaa gaaaaacaca caagatgtag tatcagaaag aaaaaattcc ctagattctg
     6241 aattcaagat acctgcaagt ggtgtgtgtt atatggctga gtttataaag gaatctaggg
     6301 aaatcataaa agaattagat aaacattttg aaagttgtct agatgtttta tctaaggcaa
     6361 gactctaaat attttgaata tgctgaatca agcatggttt gccaatctgg gaaatcagta
     6421 tttttaacta taaataaatc aaattcatta tcaggaatag ctaaaaagtc ttcttctgaa
     6481 ttgactatgt aattaccaaa tgcaagtagt tcatcaaaag aatcaaaatt agtgtgttgg
     6541 ttcataaatt tcttgctaag aattattgtg cgaatcttat caaagtcagg ttttaaattt
     6601 tgttccattc tttcaatttt cttttgaaat tgctttaaat ttctaatgtc aatattttta
     6661 ctcataagaa cacctccttt caatagaata ttagcataaa attgtggtga attctgctat
     6721 tgtcgaacga ttgttgaagg atattgtata ataacataga atttactata ctataaggag
     6781 gtgattatgt gggatttgag ataagtggta atttgaattt tgacagtgtt attgatgatt
     6841 taaaaaaaga agttgaaaat aaccctacta tatttacatc acaaaatgtt ggaaataagt
     6901 tcaaagaaaa atgtaaaata tgcgaaaaaa tatctgaatt tgaaatacta gaagatggta
     6961 aagttaaatg tttagaatgt gggactgaat ttgaattgaa tcttaaagta gagtaaacaa
     7021 aaaaagaatc tcaattatga ggttcttttt ttattcccaa aacaaacaaa taaagaggtg
     7081 gtgatgtgca agatgtcaaa gaaaaggtaa aacaagatta cttaaaagga atgaaacaaa
     7141 aggaaatatc atcaaagtat gacattagct taaacacttt aaagtcatgg ataaaaagat
     7201 acaactgggc tagtgaaaaa aagaagggtg cacctataaa taaaagaggt gcaccctttt
     7261 ctaataaaaa ttcagttggt catggtgctc caaaagagaa taagaacgct gaaaagtttg
     7321 gtttcttctc aaaatatcta cccgaagaaa ctagggaatt gatacaagaa atatccataa
     7381 aagataaatt tgatattctt tgggagcaga taacaatcca atacgcagca ataataagag
     7441 cacaaaagat aatgtatgtt aaagacaagg aagaaatgat taaagaatta aagaaacatg
     7501 aaagtacaga aaatggagag aagatagagt atgaatttca atttgcatgg gataggcaag
     7561 catcttttct taatgcacag agtagagcta tgagtgaact tagaagttta attaaacagt
     7621 atgatgaaat gattcataag gattggaatt tggctacaga ggagcagaaa acaagagttg
     7681 agaagttgaa atgtgaagtt gataacctaa gtaaagatga tattggagat gatgagttga
     7741 aaataagtgt agattatggt gatagaaatg atagttagaa tagattttaa tccagatttc
     7801 aaggaagcca attttactaa aaaaagatac agagcaatga aaggttcagc agggagtgga
     7861 aaatctgtta atgtagcaca agactatata ctaaagttag gagataagaa gtatcaagga
     7921 gctaatctat tagtagttag aaagtcagaa gctacacata agtattcaac gtatgcagag
     7981 cttacaggag ctataaatcg tatttatggt aaacaagctg ataagtattg gaaaactact
     8041 ttaaatcctt tagaaattaa gagtaaagtt actggtaact ctataatttt cagaggagtt
     8101 aatgatgcaa aacaaagaga aaaattaaaa tcaattaact tctcgaaagg aaaattaaca
     8161 tgggtttggt gtgaagaagc tacagaactt atggaaagtg acatagacat actagatgac
     8221 cgtttaagag gtattttaac taatcctaac ctatactatc aaatgacatt tacatttaat
     8281 ccagtctcag ctactcattg gataaaaaga aagtattttg actataaaaa tgatgatata
     8341 tttactcatc atagtactta tctacaaaat agattcatag atgaagctta ctacagaaga
     8401 atgcaaatga gaaaagagca agacccagaa gggtacaaag tctatggtct tggagaatgg
     8461 ggagaaactg gtggagcaat acttaaaaat tatgttatac atgaatttac tacagaattt
     8521 gagtattttg acaatatgag gttatcacaa gactttggat ttaaccatgc aaatgtagta
     8581 cttagaattg gctttaagga tggagagtta tatatatgta acgaaatata tgtacatgaa
     8641 atggatacct cagaaatcat aaagattgca aatagtatag gtttagaaaa gactctattt
     8701 atgtactgtg atagtgctga accagataga attaagatgt ggaagaatgc aggatataaa
     8761 gctaaaggag ttaaaaaagg gccaggaagt gttaaagctc aaatagatta tttgaaacaa
     8821 ttaagaatac atgtacatcc tagttgcact aataccataa aagaaataca acaatggaaa
     8881 tggaaacaag acgaaagaac tggattatat cttgatgaac cagttgagtt tatggatgat
     8941 gcaatggctg ctcttagata ttctatagat aataagctta aaaataatgg aataagcttc
     9001 ttaaagtaaa ggaggtgtta aatatttata taagtgaaac agatttaata aaagttcagt
     9061 taaaaaaaga gagcaccttt aacctagtaa aagtcataga acactacatc ttaaaacata
     9121 ggccagaaaa atataaacaa ggagaagaat actattatgg taatactgat gtaaacaata
     9181 agagaagata ttatctctta gatggagcta aggttgatga ttttactaaa gttaataata
     9241 aagcaattaa caactaccat aagcttttag ttgaccaaaa ggtaggctat agtgtcggaa
     9301 atcccatagt atttaatgca gatgatgata atctcactaa gcttttaaat gacttactag
     9361 gagaagagtt tgacgataca ataacagaac tatatctcaa tgctagtaat aaaggggttg
     9421 aatggttaca tccatatatt aatagaaaag gtgagtttaa atatgtaata attccagctg
     9481 aagaagcaat tcctatttgg gatagtaaaa gacagaggga attagttgca tttattaggt
     9541 tttattatat tgaagatata gatggaaata aaataaaaag agttgagtac tacacagaaa
     9601 atgatgtaac ttactttgtt gaaagaggta atagttttgt tcaagaattt ttatatgatg
     9661 aatatggaaa aatgactgat atacaagaag gtcattttag aataaataac aaagaacagg
     9721 gatggggtaa agttccattt atatctttta aaaataatga aaagtgtgtc tcagatttaa
     9781 ctttctataa atcattaata gatatatatg acaataatat ttctacacta gcagataact
     9841 tagatgaaat acaagaggtt atttatgtat taaaagaata tccaggaaca agtctacaag
     9901 agtttataga taatataaga tactataaat caattaaagt agatggtgga ggtggagttg
     9961 ataaactaga gataaatata ccagttgaag ctaaaaagga gcttcttgat agattagaaa
    10021 agaatataat tatctttggt caaggagtta atccagaatc tcaaaacaca ggtgacaaat
    10081 cgggtgtagc acttaaattt ttatattcat tactggacct taaatgttct aagactgaaa
    10141 agaagtttaa aaaagcaatt agagagcttt tatggtttgt gtgtgagtat ttaaagataa
    10201 gtggtagtaa gagctatgat tataaaacag ttcaaattac ttttaatcac tctatgataa
    10261 taaatgaagc tgaaaagata gatatggcag ctaaatcaac tggaattgta tcagatgaaa
    10321 ctattgtttc taaccatcct tgggtcgagg atgtaaatga cgaacttgag agacttaaaa
    10381 aacaggaaga tactcaaaaa gagtatgatg atttaattcc taataatcaa gatggtgtta
    10441 tagatgaaac ataaagatta ttggaggaag agatttgaac aattagaaga agctcaaaat
    10501 aacaaaagtg taaaatatta tcttgaatta gaaaagcaat ataaactagc tatgaatagt
    10561 atagaaaaag atatattagc atggtacaac agatttgcca aaaatgaagg aatatcttta
    10621 ttagaagcta agaaactact aaatacaaga gaactagaag agtttaaatg gagtgtcgaa
    10681 gaatatatta gacatggtaa agaaaatgct ataaatcaaa agtggatgaa agagttagaa
    10741 aatgctagtg caagagttca tataacaaga cttgaagctt taaagttaca aatacagcaa
    10801 caagtggaag tgctttatgg aaatgaactt gatggtattg ataaattaat gagacatatt
    10861 tatacaagtg gatattatca tgcagctttt aatgttcaac aaggagtaaa cgttggttgg
    10921 agtttaatga atcttgatac taatagaata aataaagtta tctctaaacc atggactagt
    10981 gatggattaa attttagtga aaggatttgg ggcaaacata gacctgcttt aattaatgag
    11041 ttacatacta agctaactca atcaattatt agaggtgaaa atccaaagaa cctagtaaat
    11101 gacttttcta agagatttaa tgtatctaaa tcacaagcta agaatttgat aatgactgaa
    11161 tcagctttct ttgcatcagc ttcaagaaaa gattgtttca atgatttaga tgtagagaag
    11221 tatgagatta ttgccacact agacttaaaa acatcaaata tatgtagaga gttagatgga
    11281 aaagtatttg atatgaagga ttatcaagtt ggagttacag ctccaccatt tcattgtcgt
    11341 tgtaggacaa caacagctcc ttggtttgag gatgaagaag gttacagagc agcaaaagga
    11401 gaagatggaa aaacatatta tgtaccatct agtatgaagt ataatgagtg gtatgagaag
    11461 tatgttaaac ataatagtat cttagaaata aaaaatagtg ctataataga tagcataaaa
    11521 gaagatatta aaaatggtaa atataattta aatattcatg atgggaaaca aggaaaacat
    11581 ttaaaagagc ataataatta tatagaagga agaagctatt taactataac aaaagaagaa
    11641 gctcaagaac ttgtgaataa gcatgctggc aatggaatca taaaatttaa tcgaagtgga
    11701 gaatgggata aaaaagaact tatagaagtt gataagaata taggggtcaa tgttaataat
    11761 attacgggtg aaaaaactct tacaaataag tttaagatac attattccaa aactggaaca
    11821 catatagtac cagctttata aggaggaaaa ataaaatgaa attatgggaa tatgtaggga
    11881 aaaatgttca aataacttgt gtagataagc aaataataag aggaaagtgt gatggatata
    11941 cacaagcttt agataatgaa ccagaaatag caagtatatc aatagctagg gatggttatg
    12001 gaattgaagt ttatgaaaat gaaatagaat ctatcgaaaa tataaataag gaatgactaa
    12061 gcatgtactt aaaaataagt agatgctttt attttgtaaa aaatgaaagg agaaatttaa
    12121 aatggattgg ttaaaagaat tgctagaagg aataaaaata gaggataaca agattgatgt
    12181 agcttcttta caaaagtcta tagaaaagaa aataaaagag actacaatta ctcaagaaga
    12241 ttatacaaat cttgaaacac agcttaatac agctaatgaa actattaaaa agtttgaagg
    12301 aggtatgaca aaagaagatg tagagaatct aaaaacaact tatgaaactg ataagaaaac
    12361 tttggaagaa acctacaaaa aagaaattga agaaaaggac tttaattact ggttaaatga
    12421 tgcttttaag tctattaaat gtagggatga aatagcgtta aaagctcatt tagatataga
    12481 agcactaaga aatagtaaag atagacaaaa agcttttgaa gagcaaataa accctttgaa
    12541 acaggataaa gattatttgt ttaatgcaac actagaaggt gaagagccta aaatagatac
    12601 tataacacca gggcaagagc ctaagataaa tgattttggt tttaatttta ctggggtaag
    12661 acctcatgaa aataataata aataggagga aataaaatgg cagcactaaa ttatgcaaaa
    12721 gaatattcaa atgttttagc acaagcatat ccttatactt taaacttcgg ggatttgtat
    12781 gcaacaccaa ataatggaag atatagatgg actggttcta aaacaataga aataccaact
    12841 atatctacaa ctggaagagt agattcaaac agagatacaa tagcagtagc tcaaagaaac
    12901 tatgataatg cttgggaacc taaggtatta actaatcaaa ggaaatggtc aacattggtt
    12961 catccagcag atataaacca aactaattat gtggcttcaa taggcaatat aacaaaagta
    13021 tataatgagg aacaaaagtt tccagagatg gatgcttact gtatatctaa aatatatgct
    13081 gattggaccg cattaggtaa tacagcagat acaactgttc ttacaacaac aaacgtatta
    13141 gaagtatttg ataagttaat ggaaaaaatg acagaagcta gagtacctga aaatggaaga
    13201 atattgtatg ttactccagt agtaaataca cttatcaaaa atgcaaaaga gatacaaaga
    13261 acagtaaata taaaggatgc aggaacttct cttaatcgtc aaacaactga tattgacaca
    13321 gttaaaataa ttaaagtacc atctaatcta atgaaaactg catatgattt tacaactgga
    13381 tggaaagtag gagcaggagc taaacaaatc tttatgtcct tagttcaccc aagtgcaata
    13441 attacacctg tttcttatca gttctctaag ttagacgaac caacagcagt tacagaggga
    13501 aaatacttct actttgaaga aagttttgag gatgtattta tattaaataa aaaagctgat
    13561 gcaatacaat ttgttgttga aggagctgga gcataatggc acaagtaagg aaattaaata
    13621 gaatattaac catagaagag tgtaaaatag atgatttctt agagatggga tatgatttga
    13681 tagatgaaac tggtaaggta gtaaggtatg gcaagtcatt aaatgtaaaa gatttaatag
    13741 ctgaaaataa tattttaagg tcaaaagttg agtctttaga agaagaaaat aagcagctta
    13801 aagagaaaaa taaacttact aaaaagtagg tgaaaattat gggaaataat ataattgatg
    13861 atatagaaaa aagacttgaa agttttggat atatattaaa agatggggat aagtggttaa
    13921 tagattttgt aagagaaaaa atagaaaata ttattaaact agattgtaat ataaaaacta
    13981 tgccaattga attgaaagaa attgaagctg atatgatagt tggagagttc ttatttacca
    14041 agaaaaatat ggggcaatta gatatagaaa gcattaactt tgaagctgta gaaaagtcta
    14101 tatcagaagg tgatacaaag gtagatttcg ctataggaag tggctctcaa acaccagaac
    14161 aacgctttga tagcttaata gcttatctta ctgcttatgg taagaataag atattaacct
    14221 ttaggtgctt aagatggtaa gtaaaactag aaaagcaata gaaatgttat atagatataa
    14281 atgtactata gttgagtatc agccaatcaa agaccctgta acaaaacgaa ctaacaataa
    14341 agaagtgatt gtattagaaa atcaaccatg caagctttca tataaaaata tagtttctgc
    14401 tacagaagga aaattagcta agctagagca aactattaaa ctctttatat ctccagatat
    14461 agaaattaaa gcaggttcaa aacttattat aaatgataaa gagtatgtaa gaagtggaga
    14521 atcagctata tatccaaatc atcaagaaat aatacttgag ttatttaagg ataaagcata
    14581 atggctagat ggggcagtgt tgattttaga gagtttaaaa gagtttgtaa aaagatggag
    14641 aagcttacaa agattgattt agataagttt tgcaaagatg cagcaagaga attagcagca
    14701 agactcttag gaaaagtaat tagaaggaca ccagttgata caggattctt aagacaagga
    14761 tggaatggag tggcttatgc taggtcgctt cctgtgtata aacaaggaaa taattatatt
    14821 atagaggttg ttaatccgac tgaatatgca agttatgtaa atttcgggca tagaactaaa
    14881 gacggaaaag gttgggttaa aggacaacat ttcttaacaa tttcagagat ggaactacaa
    14941 agccaagttg ataagattat agagaaaaag ttattaatat tacttaaagg agtatttgat
    15001 gcttaataat attatagatg gaatatcagt aaagttagat aaatcatttg gagaaaaata
    15061 tacaatttat agtgaggatg tagagcaagg tattaatgaa ccttgttttt ttattgttcc
    15121 tttaaatcca agcaagacac catatccaag cgggagagaa ttaaagaaaa attcttttga
    15181 tgtacattat ttccctcgtt cagaagctaa gaattttgaa ataaatgaga tagctgagat
    15241 gttactggaa gaattagagt atatagaaat caatggagat ttagttagag gcacaaatat
    15301 gaattttgaa attatagaca atgttcttca cttctttgtt gattataact acttcactat
    15361 aaagagtaat aatgcagata aaatgaatac agtagagtta ttcggtggtt tgaagagagg
    15421 tgataatttt gagtaagaca ttaagcaaag gaaccgatta caagtttact aaggagcaga
    15481 tagttaattc taagaagtat gtaaatagaa aagacttatt aaatgcaatt ttaaaagaaa
    15541 atgagttata ttccttctca gaagtagagg atagaataaa taaattcatg aaaggagtga
    15601 gttagatggc tttaggtgga ggaacatttg taacacagaa taaagtatta ccaggtagct
    15661 atataaactt tgtaagtgcc acaagggcaa ccagttcact tagcgataga ggtattgttg
    15721 caatgccttt agaacttgat tggggaattg atgaagaagt atttcaagtg actagtgatg
    15781 attttgagaa gtattcaact aagtattttg gatatgatta tacacatgag aagttgaaag
    15841 gtttgagaga tttatttaaa aatataaggc taggatattt ctataaatta aataaaggtg
    15901 ttaaagctag ttgtagtatt gctacagcta gatgcagtgg tataagaggt aatgacttaa
    15961 aagttatagt aacaacaaat atagatgata atgctaaatt tgatgttgta acacttttag
    16021 ataataagaa agttgatact caaatagcta aagttattac agaactgcaa gataatgatt
    16081 atgtcacttg gaagaaggaa gcaacattag aggctacagc aggattaact tttactaatg
    16141 gaactaatgg tgaagctgtg acaggaacag agtatcaagc ttttcttgat aagatagaaa
    16201 gttactcatt taatgcacta ggatgtttgg ctacaacagc agagattaaa agtttgtttg
    16261 tagagtttac taagagaatg agagacaaag ttggggctaa gtttcaaact gtattatata
    16321 aaaagaatga tgcagattat gaaggtgtag tatctgttga aaataagatt aaagatactg
    16381 gattattaga atctagctta atttactgga ctactggagc tatagcagga tgcgatataa
    16441 ataaatctaa tactaacaag cgatatgatg gtgaatttga tgtagatgtt aattacactc
    16501 aaatacattt agaagaagct ttaaaaactg gtaagtttat atttcataag gttggagatg
    16561 aagttcatgt attagaggat ataaatactt ttgttagttt tacagatgaa aagaatgacg
    16621 acttttcaag taatcaaagt gttagagtac ttgaccagat tgctaatgat atagcgactt
    16681 tatttaatac aaagtacttg ggtgaagtac caaatgataa atctggtcgt atctcgtttt
    16741 ggaatgatgt agttaagcat catgaacaac tgcaaaatat gagagcaata gaagatttca
    16801 aagctgatga tgtttctgta gaacctggaa gcgacaagaa gactgttgta gtaagtgatg
    16861 ctgtaaaagt tattagtgct atgagtaagc tttatatgac tgtttcagtt agttaacaat
    16921 aagaaaggag aataataatg gcacaacaaa taaaagcaag agatacaata agtgcatcta
    16981 aggcagagtg ttttgtaact ataaaaggta aaagatataa ttttatgcaa gctattaact
    17041 tagaagctaa aatggaaaag aataagagtg agatacctat attaggtagt actacaaaag
    17101 gaaataaatc aacaggaagt aaatattcag gaaatgcaac attttattat aatacctcta
    17161 tatttagaga attgttgtat gagtataaag aaactggtga ggatatttac ttcgatatac
    17221 aaattaccaa tgaagaccca acaagttcag tgggtcgtca aactataata ctggaagatt
    17281 gcaatatgga ctcaggcata attgctaaat ttgatgctga tggggagtat ttagatgaag
    17341 atatggattt tacttttgag aattggaaat tagttgagaa atttaatata gcaaatggta
    17401 tggagtaaaa tacacattta tgatttatgt atgtgtattt tttatttata agaataggag
    17461 atgattaaaa ttaaggataa atatgagata aaagattcaa tttcttttga ttatagcaat
    17521 aaaagacctt tggaagaacg tgtcagcgag atgtataaaa aggcaggaaa atatcttata
    17581 gatatttcag ataagttagc aacagataca attgatggtt cgtcattaaa gccaataatc
    17641 ataaaatttg aaataaatga agctggtgtt gcaacaatag aaaaacaaac aaaatatttg
    17701 gtgatggagg tagaataaga atatgggaga tttaaacgct tttttaagtc aaaatgcaat
    17761 aaaagtagag aatagaaagt atgtggcaag tgaaaggttt ataggagaag atggaaaagc
    17821 aatcgaatgg gaacttaaag caatagattc agatagagat agacaattaa gaaaagattc
    17881 aactataaga gtacctgtac taaataaaaa agggaaagca acagggcaat acactagtga
    17941 aacagatttt aatacttata ctttgaaact gtgtgtagaa actgtagtat ttccagattt
    18001 acatgatgca gaacttcaaa atagctatgg tgtaatggga gcagaggaac tattaacaac
    18061 aatgttaact cctggtgaat acacagacct ttcaagtgag gtaggagaag tgaatggttt
    18121 tgataggact tttgaagata aagtagaaga agcaaaaaac taattgaagg aggcgattat
    18181 gatgctagtg tagctcatta ttgccttcat aaattcaaat ggaaaccaca tgaatataca
    18241 gatttaccag acttcgagag ggcatttgtt gctgcttcta tagatattaa agtagaagaa
    18301 gaaataaaag aagaaaaaaa gattgctaaa gaagctagaa gaagtagaag aagataaaat
    18361 ataggtaaaa tatgtaagaa ttatatgtta taataattgt agcaagaaga tgtaatctac
    18421 aatttataga gtggagttca tactgggata aaacctactt cctaatgaaa ggaggtggga
    18481 agtatggaca attttttact tagtatatta gctagcttga tagctagttt aattggatat
    18541 atcgtttgta gatgtatcaa aaacgtaaaa agccactcta ctcgtggcaa gagtaaaagt
    18601 ggctgggaac ttggttttaa aataaagttc cgcaaattta aataatttat attttttaaa
    18661 attatgaact tcactctacc gctaaataga ttgtagttct tcttgccttt attataccac
    18721 aaattagaaa aaatatgcaa agtacttgtt ttaatagtaa gtactttttt tatgtgaaaa
    18781 agaaggtgat tgaatgaata aagatataga acttacagaa aaagatttat attgtattgc
    18841 tagacatatt caagtgtatg tgtttaaaaa agatgatgag gtcataaaaa aggaagataa
    18901 tccatgttgc agatgcaagc ataaatttaa gtattatgag aatagtgtat gtatctatca
    18961 ttgttcagta tttcagaagt tatctaaaat tacaggtctt aaaatgggtt ttggagtcaa
    19021 actataatgt aaaaattttg aatatatagt ataatattct tataaaatat aatttagggg
    19081 gatattatga gagaaggaaa gagaagaaga ggatgtctat tttggtttat cattattatt
    19141 atattttctg gggttgttgg agcaatagca gggaacagta ctaataatgg aagcactgaa
    19201 aaacaaaaag aagatttaac taaatatatt ggtgaagaag gtaatatagg agatttaaaa
    19261 ttaactgtta atagtatttc aaaagctagt gaaataccag tagcttctgg ttatttggcg
    19321 tacactccag atagtggcaa atatggtatt ataaatttaa caataaaaaa tcaaactaag
    19381 gaaagccaat cttttatgct aaattcattc acattaatag gaccagataa ttcaaaatat
    19441 gttccatctt tattgattga tgtaggaaat aaatatataa ctatggatac tgtaaatcct
    19501 aatttagatg taacaggtaa tattgcattt gaaattccta aggatttact agtttctgat
    19561 tgtaaactaa aatatagtgg ttcagagcaa gaatttatat taaaagaaaa ataatatata
    19621 gatttaaaat caaaaatact aaaaacactt actaatgtag gtgttttttt aatgaaagga
    19681 tgtgatgata atgtaaaaat tttactaata tagtataata ttcttataaa attattatac
    19741 tgggggggga atgttatgtt ttgttcaaat tgtggtgcag aaatcacagg cataggcaag
    19801 ttttgttcaa gttgtggggt tgctgtagaa actgaaatta ttgaagataa taatattaaa
    19861 tcaaatgact taatcgttga tgctaatgga atagaaataa atatgactga aatttataga
    19921 aaatatagaa aagaaaaagt aaacgcaata aaaaatgtaa tggaaataag tggtttgaat
    19981 ataaaggaag caaaaaaaat agtggatttt tcttttgaag aattaaaaat taattttatt
    20041 gatgatacta tgagtaattc agaaaaggaa aaaataatac aaaatcaaaa tagaaaaaat
    20101 aatattgaaa aagctcaaca agaatcagtt gcttgttgcc ctaagtgtgg ttcaacatcc
    20161 ttgacagctc aaaaaaaagg ttttggtata gggaaagcaa tggtaggagc tagtctaact
    20221 ggtggtatag gtttagtagc tggaaattta ggagcaaaga aagttagagt tacatgcttg
    20281 aattgtggga agcagttttg ggcaggcaaa aaatagatgt attaattata aaaacactta
    20341 ctaatttagt aagtgttttt attatctaaa ttaacagaaa ggagagtgaa aaaatggcta
    20401 caatacagac atcaattcgc atatttgatg gaatgacacc tgcgtttcgg catatgacaa
    20461 ctgctatgaa catagtactg agttcatttg agcaattaca aagaacttct agtaatgcag
    20521 taaatgctaa tagcataata agagctagag aagaactagc aagagcagaa gctggatttg
    20581 atagattaga acgacaaata agagaatcag ataatcaaca gcgaaaactt aatgaggata
    20641 taaataaggg tgcaagttct acagatagat tagttggaag tgcaaagaag ctagcagcaa
    20701 cttatttagg tataagaaca ttaggaggtc taggaaattt aagcgaccag atgacaagta
    20761 ctaatgcgag acttggcatg ataaatgatg ggcaacaatc agatgctgga cttaataaaa
    20821 tgatatttca atcagctgaa aggtcaagag catcttactt agatactgca caaatagtga
    20881 gtcgtatagg catgaacgca ggaaaggcgt ttagcagtac aaaagaaatt gtaggttttg
    20941 cagagcaatt aaacaaaaaa tttgtaatag caggcgcaag tactgaggaa atgaactcgg
    21001 cattgttaca gctaacccaa gggttgagtt ctggcgtatt aagaggtgag gaactgaatg
    21061 ctgtgtttga gtcagcacct aacatcatcc aatcgattgc agattatttg gacgtggaca
    21121 taggaaaaat aagaggaatg gcatcagagg gaatgttaac agcagatatt gtaaaaaact
    21181 cattacttgc agcagcagag cagaccaatg cagagtttga aaaaatgcct tacacatttt
    21241 ctcaaatttg gacttcaatt aaaaataatg caatcatgat atttggtgtt atacagaaaa
    21301 aaattgaaca gtctatgtct agtaagggat ttcgaacctt tatagataat tttataaact
    21361 ctttgtatgt acttggaaat gttgcttata acatttttaa tgaaattata agtatattag
    21421 ggagcccgtt ttttcaagca tttgtaaatg cgattattgt aggtgttagt ttaatagtgc
    21481 aggcactagg ttggataata acacaggcat taaatattgc taatgtgttt gctcagaact
    21541 ggagtattat tgcaccaata gtacttggag ttgcggctgc tatgttagtg tataacaatg
    21601 cgttattact tagtattgcg aataaagtta aagatattgc actatctgcc aaatctttag
    21661 tcatgagctt tgcacatata gtagcagagt cttatagagc agcagcttta gtagcaacta
    21721 caatagcaca agacggatta aatgcagcaa tggcagcttg tcctattact tggattttat
    21781 atggaattat agctatagtt gttgcatttt ttgtagctat agcaatattt aatcacttcg
    21841 caggcactag tgtatctgct ataggtgtag ttgcaggtgc aatatcagtt gcagcctctt
    21901 tcataggaaa cttatttatt gcaacaggaa atttaattat agatattgta gctttaatat
    21961 acaatacttt agcaggcttt gcagagttct ttgctaattt tttagacgac ccaattggct
    22021 ctgttataag agcagtatcc ggaatggcta atgctgtatt aggcattata agaagtatcg
    22081 caagtgcatt tgataccgtg tttggttcaa acttggcaga tgcagtaagt ggatggcaag
    22141 ataaattgca aggttggact gataaagtag caggagaagc taaaataaaa gtcgaaagaa
    22201 tggaccctaa taaactgcat tttgacagat ttaattatgg aaaagcatgg gacgcaggat
    22261 ataaatgggg agataagtta gaaactaata taaaagataa atttgatatt agcaaaatgg
    22321 cagaagatgc aaagaaaaaa ttaggattgg atgatttatg ggataaaaaa tatggattag
    22381 gagatggatt tggttcagca ggattaaact ctcctctcaa tgatgcagca aaaggagcaa
    22441 aagacactgc gggaaataca gcaaagatgg ctaaaacaat ggataaaagc caagaagact
    22501 taaaatactt aagagacata gcagaacagg aaacaatcaa tagatttaca ggagtaaaca
    22561 taaaaattga tatgaacaac acaaacaaca taagtaaaga taatgactta gatggaatag
    22621 ttaatgttct aactgaaaag ttaaatgatg caatggctgt ttcagccgaa ggaatagttt
    22681 aggaaggaga gtgagaaaat ggcttatgat ttttacctag atggagtaca actaccaatc
    22741 gcaccaccaa agcttgaagt caaagtgaca aataaaaaca agacagttga tttaataaat
    22801 gttggagaag taaatatatt aaaaaaagaa ggattatctg aaataagttt tgaagcagaa
    22861 tttacacata ataaactacc attttatcgt ggaactttta gggatgttca attcttttta
    22921 agtaaactgg aactactaaa aactgattgt aagccatttc aatttattgt atcgagggaa
    22981 ttaggtaata aagtactatt taacactaat ataaaagtat ctcttgaaga gtatgctatt
    23041 tcagaagatg cagataatgg ctcagataca aaagttgcaa taaaattaaa acaatataga
    23101 gattactcaa ctaaaaagtt agttcctgca actcctgaaa agacaaacta tggtaggact
    23161 ccccctccag tcatgaaacc aaaagaattt agaccagatt catccaataa gccaaatggt
    23221 aaaacatata cagtaaaagc aggggattct ctttgggcaa tttgtcaaaa gcaattagga
    23281 aatggttcgt tatacaagaa agtatatgag ttaaataaaa caatgatgga taaagctaac
    23341 aagggaaaaa aattaagtaa atacaccatc tataaagggc aggtgttgag gcttgtctga
    23401 tgatttagtt ctggcgaatg atagagatat aaggctagtt attgcacatt gggaagattt
    23461 ctacgaacct gtagttttgg atggtatcac atgggaaata gagagacgag gaacaccatc
    23521 taaacttgaa tttacaatag tcatggatga tatattagag ttttgtgaag gtaactctgt
    23581 aagactatat tacaaaggtg taggtatatt ctatggatat atatttcaga aaaagagaga
    23641 taaagaaaat catatcaaga tagttgctta tgaccagcta agatatttta agaataaaga
    23701 tacttatgta tatagtaata aaacagcaag tgaacttgta aagatgttgg ctaaggattt
    23761 taaattaaaa tacaatgtca tagaagatac aaaatataaa atatcgagag ttgaagaaaa
    23821 taaaacactc tttgatatgg tcttaactgc actagatgat actctaagag agaaaaagga
    23881 aatgtatgtt ttatatgatg attttggaag aataacatta aagaatgttg cttctatgaa
    23941 acttgatacg gttatgaaca atgatgtaat tgaggacttt gactacaatt cttctattga
    24001 tagtgatact tacacaaaga ttaaacttgt gagagataat gaagaaacag gaaaaagaga
    24061 tgtgtatatt gctcaagact caacgcatat gaggagttgg ggaatactac aactatttga
    24121 tacagttgat aaaaatatga gtgaagcaga gataaaacaa aaatgtgata tactcctaaa
    24181 actatataat aagaaaacta agtcattaag tttaaaaaat gtgttaggag atattagagt
    24241 aagagcaggt tgtttagtac ctgttttttt gtcgttagga gatattgatt tacaaaatta
    24301 tatgttagtt gagaaagtaa aacatacatt tgaaaataac agtcatttta tggacctaac
    24361 tttggttgat ggagacgaat ttgcttctta ttcttcatca agttatagta gtggaaatac
    24421 taataataaa aatgagaaac aaaatggtcc tgcacaaagt actacaagta aagaagatac
    24481 tgatatggct aataagatta ataaactact taaaggtaaa ttatcaaata caggaaatat
    24541 atttgttaaa tattcaaatg cttataaagt taatccagca ctcatggctg ctatatctat
    24601 gcacgaatca gctagaggga cttcaaatat tgcaaatact aaaaataatt tctttggaat
    24661 gaaaaaaaat ggagattaca tgagtttttc tagtgtagac gaaggaataa aaagaggtat
    24721 aagtaattta tcaagaaact atatccatat aggacgaaaa actttagaaa gcatcagaaa
    24781 taaatattct tctagttcag acaaagaatg ggtaaaatgt gtaggtgcat tttataagca
    24841 aataacagga agtacttata attctaatag tgcaggcaca ggagttggaa gtaatgaaga
    24901 agcagaaaag aatttaaaag atttaactta tcaagttcaa aacaataatt ctaatacatc
    24961 aacaaacaat aataataaag taagtaaagt tattcaagaa gcaaaaaatc aacttggcaa
    25021 gccttacaaa tggggtggta atggtccaaa gagttttgac tgtagtggtc ttatggtgtg
    25081 ggcatttaaa agaggtgcag gaataaatct caaaagagtt tcagcagacc aatcaaaaga
    25141 tagtagagga aaactattat gtaacataaa tgatgtaaaa gctggtgatt tagtattctt
    25201 tgcatacaac aaaggaaaag gaaatgtaca tcatgttgga ctatatatag gaaatgacca
    25261 atatattcat gctccacaaa ctggtgacgt agtaaaaata agtagtttaa gtggtagaca
    25321 aaagaaaaag catgattttg caagagctag aagattcttt taagtgaggt gataaagtgt
    25381 cacaagaatt attgcaaata attaagaagg ctgcaatgga tgcagtagaa acaagcaatc
    25441 caatgcaaat tgcatttgga actatagaaa gtgttaatcc tttgatagtt aagatagaac
    25501 aaaaagcatc ttttgaagaa ttttttctaa tacaaacaga gacttttaaa agatatacag
    25561 ataaaaaaat aggggataaa ttagtcttaa ttaggatgca aggaggacag caatatttga
    25621 ttttagatag gatgtgatga aatgttacca acagataaca ttgactatga tatagaagat
    25681 gtatcgataa ttaattttga tgttagacaa gaaccaagta agacgtttaa attgaatata
    25741 gagaaaaata gagtagatgg tatttgtgat gatgtagaag cattaaaaca aaccattttt
    25801 ttaattttaa atactgaaag gtatgagcac cttatttatt ctagaaatta tggtgttgaa
    25861 ttaaatgatt taattggaga acctatttca tatgtaatac ccgaacttga aagaaggata
    25921 acagaagcac taattcaaga tgataggatt gaaaatatag ataattttga gtttcaaaat
    25981 ataaagggta aagtacaatg tagattttca gttcatacaa aatatggaaa tataaaagca
    26041 gagaaggtgg tgagtgtata attgtttgag ttaatgacat ttgaaaatat aattaaaaga
    26101 atgttagata gtgtaccaga tacttttgat aaaagggaag gttctataat atataatgct
    26161 cttgctcctg ttgctataga acttacagaa acatacattg ctatggatga attactagac
    26221 caaacattcg tagatactgc tagttattat tatttagaga agagatgtaa agagagaggt
    26281 attacaccac ttgaagccac taatacaatt gcaaaaggag tttttaacat agatattcct
    26341 attgattcta ggtttaatct aggagaatat aactatgtag caattgagag aatatctgaa
    26401 ggtatatata agatgaaatg tgagactgcg ggacctattt ttgagttggg acaactaata
    26461 cctatcgaat atatagacaa attagaaaca gcagaactga cagaaatact gataaatgga
    26521 gaagatgaag agagtgagga tagtttaaga caaagatatt atgatagcct aaattcacag
    26581 agctttggtg gaaatatgca aaattataaa gatgaagtta acaaaataca agatgttgga
    26641 ggagttaagg tttatcctgt gtgggacggt ggaggaactg ttaagttagt aataattaac
    26701 tctaatttca aagtaccatc agaggattta gttaatttag tgcaagaaga aattgaccca
    26761 attggacatc aaggacaagg cttaggatta gcaccaatag ggcataaagt tactgttaca
    26821 ggtgttgtaa gtacaactat aaatatatca gcagagataa catacaaaaa tggctacact
    26881 tgggagaata taaaatcaat tgcagaagaa gcaatagacg actatttaaa tgaacttaac
    26941 atgagttggg aagatgaaga aaacttaata gtccgtatat ctcaaattga aactagatta
    27001 cttagtattg atggagtatt agacattgca aatacaatga taaatgaggt taaatctaat
    27061 ctaacaataa atagtaacag tatagtagtg agaggtgagg tagttggata aagagattaa
    27121 tctaataaat tacttaccac aaattctaca agataaagaa gaatatataa aagtatttaa
    27181 tgtaggaaat aaagaaataa aaatattaca tgataaatta aaggacctat caaatgacca
    27241 gtttttagag gacctaacta taagtggtat aaaaagatgg gaaaagataa tgtctataac
    27301 tcctaaaagt aatgagagtt tagaagatag aaggtttagg atttttagta aatatataag
    27361 taaattacct tactcagaga gatttttaag gaattggcta gatagtatag ttggagaagg
    27421 taattatgag ttaactatta ataatgctac ttataatata catcttgaaa gtgatgctag
    27481 aaatcaagat tggtttgagg aagttcattc ttttgtaagt aatattaagc catgtaatat
    27541 gactttagat tacactagag tgcttataag caaagacaat tatatgaatt ttggtataac
    27601 aaccctaatg ggtcaagaaa taactatata cccttggagt ccaccagata tagaaactta
    27661 tggagaaatt gatgtattaa ctggcaatgg agttggatac caagagataa caatatttta
    27721 ggaggtgata tattggctat agataaaagt tattacacta taattacaga tgtaggaaaa
    27781 gcaaagatag caaatgcaag tgtcacaggt aataaagtgg gatttgtaaa aattcaactt
    27841 ggtgatggag gagggagtga atatactcca actgagagtc agacagctct caaaaacgtg
    27901 gtatgggaag gcaatattgg aaatacaact acagatgaaa ctgcaccaaa ttgtataata
    27961 ttagagagtt taataccatc aagtgtaggc ggatttatga taagagaaat aggatattta
    28021 gatgatgaaa ataatttaat tgccatttct aaatacaaag agtgttataa accttctata
    28081 gaacaaggtg cagtggtaga catgaaggtt aaaactgtgc ttattgtatc taatgtaaat
    28141 aatatagaac ttaaaattga cccaacaata atctttgcaa cactcaaaga tatacaagac
    28201 ttagaaacta aaataggtac tgttaatact aaaatagata caactaaaac agaattaaca
    28261 aataatttag aaactgctaa aacagagtta aatactagaa ttgacacaga aaatgagaaa
    28321 caaaatatta aaattgacca acttattgca ggtggctcta atgtggcatc tactcaaaca
    28381 ataacaattg acgattgggt tgaggatgca gaaaatggat tcaaagcaac tgtaacacat
    28441 agtttattaa cacagagaat agttgtaaat attatagatg ttactacaaa agaaaatgta
    28501 gttacaaact ttaaaattat agatgataat tctatagaaa ttagaagtga agtaaaagct
    28561 gagttaaacg tttatgtgat aaatggaaat gcagaaactc attttattaa tgcgactgtg
    28621 gatgataaca gagtgtctga aatgactact tattcgtcta agaaaatcga ggatagattg
    28681 attaatatag aagaaaaagt aaatggtggt ttatctagta ttgcaacaag tgtaaatgag
    28741 ttgataactt attgttaaag gagagtgaga aaatgcagac agaatggaac tttgggtaca
    28801 atggttcgcc acaaagtgtt atattgaaac ctggcaaata taaatttgaa tgctggggtt
    28861 cttctggagg tatcaacaat tcttcttggt atactgatgc taaaggcgga tattctaaag
    28921 gtgaaattac attaaaaaaa caaactacat tatatgttta cgtcggcgaa agtggttttg
    28981 cttcttcatc tacgagtaat aacactaaaa gtggttttaa tggcggtggt aaaggttatt
    29041 taaatcaaca gattatgggt acttattatt ctatgtacgg tggtggtgct accgatataa
    29101 gacttgttgg tggtgcttgg gataatgagc aaggtttgct atctcgtata attgtcgcag
    29161 gtggtggcgg aggttcatat tatccttcca ctggtggtgc aggaggagga ttagaaggag
    29221 gtactgggta tagttctaat gacagatacc gtcccggtgg tactcaatat caaggtggta
    29281 ttggtcgtgt aaatacagaa aacggaagtt ttggaaaagg gtgttctgtt aaagattcaa
    29341 ctggcgaagg cggcggaggt ggctggtttg gtggtgcagg catgaatggt gtaggagcag
    29401 gtggaggtgg aagtagttat gtattgacta aagacagtta taagcctact ggctacacac
    29461 caacatctga atattatttt gataatattg ttatgacacc gggtggaaat actgctggtg
    29521 cttatggtta cgcacaaata actttacttc aatcattacc atttttaaat atatcatctt
    29581 ataattctac acaagcaaca ttcaaagctg accacactga ccctacttta ttgactaaga
    29641 tagaatattt tatagatgat gtattaaaag aaactataac aacagattta acagaagaaa
    29701 aaacaattaa ctatacacta gaagataatg cactacacac acttaaaata gttgttacag
    29761 acagtaataa tgctacagca gaaaaagctg tcagtataag taagaatata atgccattgc
    29821 ccgaaaatgt taatttaaat gatatatcta ctaaactagt tgaagttaat gcaggattta
    29881 agactggtaa aacaagtatt ataaacactt tggcactaaa gaatatagaa gcaagtttaa
    29941 ataacacatt agtcgagttg tcagagaaaa taaaaacaag ttttgatagt tcagacgcta
    30001 gtgtacagga tttgatgaac cagttaacac aagctaataa cactataagt cagttaaatt
    30061 ctaaatataa agttgctagt ggcaatagtg ttgttaatta ttgtactaat acaaaaggat
    30121 tttattttaa tagtgactat ctgttcctat ttcctggagc cattcaaatt aacggactta
    30181 attttgttcc taatatattt tttactactt ttgaattaat tgatgatggt tattttcata
    30241 aatattttgt ttttgcttgt cgtggtattt tcactcaaga ttttgtaatt actgctcatt
    30301 attatcgtct aacatcatat ttacaagatt ttaaagttgg aggtgaggtt cttaaattaa
    30361 atgaacgtga tgtttatatg gataatagag gtatacaact tccttgttca agacaaggtt
    30421 cttcttttaa atggcaagct ataaaattta tttaataaat gaggtgataa aatggataga
    30481 gcaaatagaa taatttacga ccagacaggc aaaatactgc tacagacagg ggaagcaaca
    30541 ggagatatac tagaacatga tacaataaca gaattacatt atattgatgt tgagtatgga
    30601 agtatagact atacaagaaa tagaattata ggtataaata tagaaacaaa agaaccaatt
    30661 ttagaagaaa taccagtatt tatctcggag gaagaaaaaa gaatacaaga gttagaaaat
    30721 caaattttat taaatgaaaa taagaaagta ggaggaattt tataatgaat ataaataatg
    30781 ttgtggtaag aatattagca gagagaatat taaacggagg cttaaaccct ttaaaaaatc
    30841 gagaatttga actggatgat gtaactaatg cagaatacag aaaagcagta gaggattata
    30901 taattagaga aagtggagta gtcgaagaag cagaaccaac tatatagagg gttctttttt
    30961 attgaaagaa ggtgactaaa tgacttttaa agagttagtt aataaagtta gaaatcttgt
    31021 attagaagca aagaatgtaa ctatagaaga tacagaaaat aactttacaa gtgataatgt
    31081 agaaggagca ttgaaagagg tttttcaaaa tggagttaat gctaaaagta atgtagtaac
    31141 agcattaaac tccaaaggtg cagatgttac tacaagcgat acatgggaag aaataaagaa
    31201 taaaattgat ataaaagagg ggagattaga tttaagagaa acaacacttt caaataacta
    31261 ttcgtattta gttacaaatg gagctataaa atatattgaa agatgtagtg ggaacttcaa
    31321 aacttttgaa tatgaagaac catattttta tgtaattaaa gaaactcatc taattaaaat
    31381 taatgctatt gatgaaacag tagtttttga cattacttta gctaatgcta acttctcatg
    31441 tatctgtgtt actcaagagt atttatttat atctgacaat actaaattat ataaaataaa
    31501 taagttaaca ggaaatgaag cacagtcaat agaaggttct tattataagc tatgtactta
    31561 tggggaattt atttatggaa tatatggaga tgagacttct tcaatacttc acaaaataag
    31621 aatatcggat atgtatataa tgctaactaa agatatgtcc tctgatagaa tttatgactt
    31681 tgaaagaggc aaatttgtat gtaataataa tggtatttat gctacaacag aacactcaaa
    31741 ttcaagtggt cttacagaat gttacctaac taaaataaat tttgatttta gtgttgcgaa
    31801 aagttttaga attggaggat atttatatga aaaaaacatt aagtttttaa atgattttgt
    31861 ttttgtatct gatagtagca gaagcataga agttgagagt ggtaaaaaaa gtggattagc
    31921 aaaatatgat gcaaatctaa atttgattac ttatggtggg tcagatagat atgaaaattt
    31981 tgaaatatat aatggatata tatatacatt taattcactt tctagtagcc catttataaa
    32041 aataaattta aatactctta aagaagttaa tagctaccga acgctcatta acacaaatcc
    32101 tcaaaaaggt atgtttataa taaacaatat aattttttta ttggtggtgg cattcacaga
    32161 aatatattat caaaaaaggt ttattcagat gaaaaaggag aggaattatg atttatttag
    32221 gaaattttat ggagacagat gaacaaaata taaaatatat tgggatgata cactataaac
    32281 caactttact atcagaagaa gagttaaaaa atggaatttt aattgagaaa ttgccagtac
    32341 aacaatatgt agaaaataaa gaagcaaagt tatttataaa tatagatact aaagaggttt
    32401 tctatagata tacagatatt aaaagtagca tagaagataa agtaaattct acagaacaaa
    32461 caatagcaga tttaacattt caattaatga gtaatggggt gatatagtat gaattggtat
    32521 aagataataa cagatttcta taataatggt aattggacta aagagcaagt taaaacagcg
    32581 gttacaaaga ataagataac agcaagtgaa tataaagaaa ttgtaagaga ggactatata
    32641 gcgtagttct tttaaataga cttagataat ttctaagtct tattttaatg cagaataagg
    32701 aggaaatatg aacgtaacaa tagttttttt agcaacaaat atatttataa aattagtaat
    32761 attagcaata gcatttgata cactgttagg ttgcttaaga gcaataaaaa cacatcagtt
    32821 taatagctct tttggaataa atggaggaat aagaaaagta gcaatgatag catgtatatt
    32881 ttttctagca gtagttgaca ttcttacaaa gtttaacttt ttatttatgt tgccacaaga
    32941 ttgggttgat tttttgcgat taaatcatct tggaatatct gaatttttct ctattttatt
    33001 tattctatat gaaagtgtaa gtatattaaa aaatatgtac ttatgtggat taccagttcc
    33061 taaacgatta aaagagaaaa taggtagttt attagatact atgacagatg aattaaatgt
    33121 taaaggaggt agtaaataat gaaaatatgt ataacagtag gacacagtat tttaaaaagt
    33181 ggagcatgta cttctgctga tggagtagtt aacgagtatc aatacaacaa atctcttgca
    33241 ccagtattag cagatacatt tagaaaagaa gggcataagg tagataatat gcccagaaaa
    33301 gcagtttaaa actaagaatg aagaaaagtc ttataaaata cctagagtta atagtggagg
    33361 atatgattta cttatagagt tacatttaaa tgcaagtaac ggtcaaggta aaggttcaga
    33421 agtcctatat tatagtaata aaggcttaga gtatgcaact agaatatgtg ataaactagg
    33481 tacagtattt aaaaatagag gtgctaaatt agataaaaga ttatatatct taaatagttc
    33541 aaagcctaca gcagtattaa ttgaaagttt cttctgtgat aataaagaag attatgataa
    33601 agctaagaaa ctaggtcatg aaggtattgc taagttaatt gtagaaggtg tattaaataa
    33661 aaatataaat aatgagggag ttaaacagat gtacaaacat acaattgttt atgatggaga
    33721 agttgataag atacttgcga atgtgcttag ttggggctat agtccaagca aagttttagt
    33781 ttgtgatata aaagattacg taccaggtca gacggaaaat ttatatgttg taggaggtgg
    33841 cgcatgtgaa aagataagtt ctattactaa agaaaaattt attatgataa aaggtaatga
    33901 tagatttgat acactttata aagcattgga ttttattaat agatagataa aagttatcaa
    33961 ctagaagtgg ttgtttgttg tgataactct attattgtaa tataatgtaa atatattatt
    34021 gtgataatgg aggatttatg tatggatgca ttggtttatg agagatttgt aagagcagct
    34081 tttagtatta aacttaataa cttgattaat agaagcgaag atttaggtgg attggctgaa
    34141 gctgatatct ttagagcagc aaataattta catgaattaa atgagataaa aataggtact
    34201 gggtatgcta ttgcaatatt taataatgag atattagagt cttgtaatgt ttctgataat
    34261 gatagcaata gaatgattga actatttgat agaagtttaa ttgcaacttc tagggaggaa
    34321 atattggata ttataagaga atatgaaact tatagaggac gatatcttac ttttaattgg
    34381 aagagataga atgtttaagt taggaagtag taattatagt tagttattac tttctttcac
    34441 atatttttat tatttcaata agattatttc caaataataa tttattcaat gataataagt
    34501 agaaaataaa attttgaatc aatataaaat ttaatgaaat gaggtagtaa aatcatggat
    34561 aaaaaaatag acatagcgaa gtttatagta aatagaattg attactatat tgaaaaatca
    34621 gataataaag ctagtttttt actattatta aatagcgcaa ttataggttt tttattttca
    34681 gggaaggaaa aaataatgaa tcacttaagt ataagtaata tcagatgttt agagatgcta
    34741 ttttacattg ttatattatg tatatttgtt atttcaattt atttttcgat tatggtttta
    34801 aagccaagga attctaaaat agaaaatgaa tataaatcta ttttttatta taaagagata
    34861 gcatctttaa ataatgaaca gtacaaagaa gcatttgaaa atgcatttaa agacgaagaa
    34921 aacttgatta atgatgcact tgttcaaata aaagaattat ctttaatctg tgataagaaa
    34981 atgtataatg taaaaaagtc agtagaagct tttattttag gtggtattat attgtttata
    35041 tatatacttg tagtagtttt taatgctatt tagaaaatgt tttatttggt tagtattcct
    35101 ctatgaagat agaatcaaat catagaagaa tactatttac atatttgtag attcttgtaa
    35161 ttgattttca tttaattttt taaatgctag ttttaaagtt tctttaaaat caggtactgt
    35221 gttttcatta aaatcttcaa gttcatcctc tcctatccat gcaagttcat tatgttttga
    35281 tgtgggttca aatttagaaa tatcataatc atttattatt tctgcgagag ttataatgcc
    35341 tttgtgtaaa ccatcacttt ttttaacttg ataaattgct agaggtatag gttggcaatc
    35401 atcttttctt gtgcaatcag taataggttc aatgtttata ttaaaatctt tttcatattc
    35461 atctttaata gtatttataa ttgaagtttc tagacttgcc ttagcacaac caaattccca
    35521 tttactcgca ttattatttc tattatcact tcttttcgca attaatattt ttgaagttga
    35581 tttattataa caaaccgcaa cacagtgtaa ttccattaaa tctactttta tatagtcttt
    35641 atctattgta tttttatcat aactggggtt tgttttagaa attacgtcac ctattttttc
    35701 aatcttatca ctaaggtttc tgtctataag tattttatcc aaagccttgt ctacagaatt
    35761 aaacataaaa gaatcaatta gtaatttact ttctcctttt ttattaaaaa aatattctcg
    35821 aaccaattca ttttcttcta tttcatcaaa agaaaaacta tcatcaaatt ctatatcatt
    35881 attttttcct ttattatgat accaaatgat agggtatagt ttatctttcc agataccctt
    35941 taatttttta tatgtaatta tatgtaattt agataaaata tcagtttttc ttgataatat
    36001 ataagctagt tcaaaactaa gaacaagtct ttctgggcat gtttgctttg atattctgaa
    36061 accagcatca atatcatttc ctaagaactc aaatatttta taattattgg ataaatcgta
    36121 catagcagaa atattttcat attgttcatt attttctaaa gcattaaaat taggatttct
    36181 tgatactata gcaatccagg ccgctccttt taatgaaata atattttgaa gtttcattaa
    36241 gtatttttca gattcagaaa atccttctag tgtagagaat atattaccac tttttatatc
    36301 tttagcagtg cttgttaaaa tatcaaagat tatatctatg tatttataaa tttcatcata
    36361 attttttagt acgataatga aaattacctc atcaccaaga actctccaaa gttgtgcttt
    36421 aaggttttca taaactctat attgtaactt acgaattata tgtgataata ccttagccca
    36481 tccgctataa tttatatttt tatataatga tgaattagct atatcaaaag aaaagaacaa
    36541 aatcacttct ggatgattag gttctttttc tttttcataa atatcagtat taatattttc
    36601 ttgaagtttt tccttatact ttttaagatt aaaagcgttt tcgtttttag ctgaattatg
    36661 aacattattt acattttgag ttttatattc tttatcatct accattgtaa atatcctaac
    36721 catttccctc tattagatgc tgcataagag gaaacattaa agtattctgc aatttttgaa
    36781 gtgtcgacct tattacctac tgtgttttca tccataattc ttttatattc atgtttaggc
    36841 atcaacaagg ctgctgcaaa ttcattggat tgatactcct tattggtatt gccacttcta
    36901 aaatactgat ttccatcttg actattccat agttcatcat caatgccata acccatgtgt
    36961 aaaaataaat gtccaagttc atgagcaatg gtaaaattct ttctggtatc tggttgataa
    37021 ggagatacca ctatttcaaa tgaatcatca acttttctaa taaatccatc agagtatcca
    37081 cttaaagaac tatcttctat taccttgcct cctaaagcat caacaacttg atttatattt
    37141 actattggaa tttggatttt gtatgtttgg ataatatctt gggttatatc attaattagc
    37201 tctctgattt tgaaatccat aaaatcacat ccttatataa tattttattt aaataattat
    37261 ttatta
//
LOCUS       DAMJWS010000033        36666 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMJWS010000033 DAMJWS010000000
VERSION     DAMJWS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.033"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            293..670
                     /locus_tag="PC399_001779"
     CDS             293..670
                     /locus_tag="PC399_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2592639.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            663..1811
                     /locus_tag="PC399_001780"
     CDS             663..1811
                     /locus_tag="PC399_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2592640.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1828..2430
                     /locus_tag="PC399_001781"
     CDS             1828..2430
                     /locus_tag="PC399_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2592641.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2523..3545)
                     /locus_tag="PC399_001782"
     CDS             complement(2523..3545)
                     /locus_tag="PC399_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2592642.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3874..4056)
                     /locus_tag="PC399_001783"
     CDS             complement(3874..4056)
                     /locus_tag="PC399_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592643.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4369..4788
                     /locus_tag="PC399_001784"
     CDS             4369..4788
                     /locus_tag="PC399_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF2592644.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            4919..6760
                     /locus_tag="PC399_001785"
     CDS             4919..6760
                     /locus_tag="PC399_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2592645.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6787..8673
                     /locus_tag="PC399_001786"
     CDS             6787..8673
                     /locus_tag="PC399_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2592646.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNETRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            8681..10681
                     /locus_tag="PC399_001787"
     CDS             8681..10681
                     /locus_tag="PC399_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2592647.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            10943..12202
                     /locus_tag="PC399_001788"
     CDS             10943..12202
                     /locus_tag="PC399_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2592648.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            12263..14050
                     /gene="iorA"
                     /locus_tag="PC399_001789"
     CDS             12263..14050
                     /gene="iorA"
                     /locus_tag="PC399_001789"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF2592649.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            14052..14627
                     /locus_tag="PC399_001790"
     CDS             14052..14627
                     /locus_tag="PC399_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF2592650.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            14680..15750
                     /gene="buk"
                     /locus_tag="PC399_001791"
     CDS             14680..15750
                     /gene="buk"
                     /locus_tag="PC399_001791"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2592651.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            15968..16669
                     /locus_tag="PC399_001792"
     CDS             15968..16669
                     /locus_tag="PC399_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592652.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            16729..17235
                     /locus_tag="PC399_001793"
     CDS             16729..17235
                     /locus_tag="PC399_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2592653.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            17410..18432
                     /locus_tag="PC399_001794"
     CDS             17410..18432
                     /locus_tag="PC399_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2592654.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(18579..18887)
                     /locus_tag="PC399_001795"
     CDS             complement(18579..18887)
                     /locus_tag="PC399_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2592655.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(18947..19375)
                     /locus_tag="PC399_001796"
     CDS             complement(18947..19375)
                     /locus_tag="PC399_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592656.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(19598..21055)
                     /locus_tag="PC399_001797"
     CDS             complement(19598..21055)
                     /locus_tag="PC399_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2592657.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            21473..22387
                     /gene="nadA"
                     /locus_tag="PC399_001798"
     CDS             21473..22387
                     /gene="nadA"
                     /locus_tag="PC399_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF2592658.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            22400..23701
                     /locus_tag="PC399_001799"
     CDS             22400..23701
                     /locus_tag="PC399_001799"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF2592659.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            23682..24518
                     /gene="nadC"
                     /locus_tag="PC399_001800"
     CDS             23682..24518
                     /gene="nadC"
                     /locus_tag="PC399_001800"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF2592660.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            24774..25106
                     /locus_tag="PC399_001801"
     CDS             24774..25106
                     /locus_tag="PC399_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF2592661.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            25268..25453
                     /locus_tag="PC399_001802"
     CDS             25268..25453
                     /locus_tag="PC399_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592662.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            25480..26565
                     /locus_tag="PC399_001803"
     CDS             25480..26565
                     /locus_tag="PC399_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2592663.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            26558..27016
                     /locus_tag="PC399_001804"
     CDS             26558..27016
                     /locus_tag="PC399_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592664.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            27051..28142
                     /locus_tag="PC399_001805"
     CDS             27051..28142
                     /locus_tag="PC399_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2592665.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            28147..28371
                     /locus_tag="PC399_001806"
     CDS             28147..28371
                     /locus_tag="PC399_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592666.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            28381..28944
                     /locus_tag="PC399_001807"
     CDS             28381..28944
                     /locus_tag="PC399_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592667.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            28905..29534
                     /locus_tag="PC399_001808"
     CDS             28905..29534
                     /locus_tag="PC399_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2592668.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            29574..29711
                     /locus_tag="PC399_001809"
     CDS             29574..29711
                     /locus_tag="PC399_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2592669.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            29711..30463
                     /locus_tag="PC399_001810"
     CDS             29711..30463
                     /locus_tag="PC399_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592670.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            30476..30961
                     /locus_tag="PC399_001811"
     CDS             30476..30961
                     /locus_tag="PC399_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592671.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            30968..31117
                     /locus_tag="PC399_001812"
     CDS             30968..31117
                     /locus_tag="PC399_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592672.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(31254..32075)
                     /gene="yidA"
                     /locus_tag="PC399_001813"
     CDS             complement(31254..32075)
                     /gene="yidA"
                     /locus_tag="PC399_001813"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF2592673.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            32568..34262
                     /locus_tag="PC399_001814"
     CDS             32568..34262
                     /locus_tag="PC399_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF2592674.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      34594..34704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            34808..35179
                     /locus_tag="PC399_001815"
     CDS             34808..35179
                     /locus_tag="PC399_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF2592675.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            35276..36223
                     /gene="trxB"
                     /locus_tag="PC399_001816"
     CDS             35276..36223
                     /gene="trxB"
                     /locus_tag="PC399_001816"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2592676.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            36327..36644
                     /locus_tag="PC399_001817"
     CDS             36327..36644
                     /locus_tag="PC399_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF2592677.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT        14500 a         3867 c         6343 g        11956 t
ORIGIN      
        1 gatattactc tagtttattt taaaaaaaag tattataata ttccttgtgt gaaaatatag
       61 gaatttttat taaatagttt taatgtgtaa ataacattct acactattgt aattaaatat
      121 gtaaaaagta aaatatttaa atagatatat tttaaaatct aacttttaat attgtaaaaa
      181 aattagaaat ttaacaaaaa gtacaaaaat tcacattttt ttcataaagt gtttgactaa
      241 aaaattcaca tgttgtagaa ttaaggtagc aggagttttg agggaggtgt aaataatgat
      301 aagaaaaata ccacaagcag aactaaaagt aatgaaattc atttggaaag tagatgttac
      361 agtgacatca aaagatgtta ttgaagctat ggaacaaaaa tatggctgga aacaaaccac
      421 cacactgaca cttttatcta ggctggtaaa aagaggattc ttagatgctc aaaaaataga
      481 cagatataca cattacacaa taagagtaaa acacaaagat tatttaagtt ttgagacaaa
      541 ggatttccta agtaatatac atgatgactc tttaagaagc ttaatttcag cattacatga
      601 tgatgaaaat ataggtaaag ataagctaga ttttctagaa agttattttg ataatttaaa
      661 ggatgaataa ttatatgcta tatgtttttg aaaatcttat aaagacaact attgtatgta
      721 gtcttggtat ttgtctatta ttatttttga aaagatattt atttaagaag tttagtaaga
      781 gattcaatta ttatatttgg ttgataatag tctttagaat gttacttttt ctatttaatt
      841 atacaatagt gtatgaagta aaagaaccca aagaaaatgc attaggaaat aatattacac
      901 aaattgatat ttctacagat aataatctta tgctgtatgt agcttattta tggttatttg
      961 taactatagt cattgcagtt tatacattta taaagtatac aagatttaaa aatctagttg
     1021 ttgatgtatc ttatgatatt gaagataatg atataaactg tatctacaaa aacttgttaa
     1081 aagaattaaa tataaaaaag aaaattgaat taagaggttc tgatgaattg ataagtccag
     1141 caggtatggg attatttaaa tcttacatat tcttaccaga ctatccttat agtaaagatg
     1201 aactaacttg gattttgaaa catgaattaa tgcattttaa aaataaagat attttaataa
     1261 agtttttagt tttgtctgtt agaataatat attggtttaa tccacttgta tatgtaatga
     1321 gcaataaagt aaatctagat tgtgaattat gttgtgatga aagtgttttg gtagaatgtt
     1381 ctttaaaaga taaaaaagaa tatgcactag cacttataaa gtcaattaag tttagtaaaa
     1441 attataatag taagattttg acaaccgagt ttgataagac aaatttggaa aagagacttg
     1501 agagtattgt taaaaagaaa ggcaaaagtg gaatactaat tgctgtttta gtatttatga
     1561 cttctataac atcttttgtt gaagttgatg ctcaagttag aattgatgag ggtaataatg
     1621 gaatgaataa caatattaat gaatttttat tgaataagac gactgataaa attataaata
     1681 tttattataa agatgcacca aatgaaatta ggaaatttta tgaagataaa tgcgaattag
     1741 aaggtaaaat acctagagac tatgacataa tagaaatgga tatgaaagat tataatcaat
     1801 taataaaata attataagga ggattttatg ttaacaaaat taagaaaaat agtagctatt
     1861 atgtgttgct tattgcttac ttttacaagt gtggcatttg ctgacacaag taaagagaaa
     1921 ggaggaagta aagagactgt tccagagaaa aacctagatt cttttactct ttctgggtat
     1981 atatatacat atgaaaatgc gccacaagaa ataagagatg aacataaaca aaattgtgaa
     2041 gagataaata tagaccctaa gccagatgat gagatatttg ttccagaatc agctaatcta
     2101 atgaatgagg acaatagtaa aggtgtttat gcaagttata ctgtttctta taatataggt
     2161 gctaaaacta taacaattat gagtaatgag tatttgacta taagtacaac aaaagttata
     2221 cgaaagggta atagcggaaa agaagtaaaa gctgcacaaa tcatgttaac tttacttggt
     2281 tataatgtag gtatagatag ttcttttgga agtaagacat ataatgcagt tgtaagtttc
     2341 caaaaaaaat acggtttgtc tgcagatgga ataattggtc cagctacttg ggataaattg
     2401 ggtagactaa ctgaccctac tctttcatag gattaaaatc taaaaatata ttaaatagtg
     2461 tctcaatatg atttttcaat ttaaatgaga cactatttaa atataaatgt tataaaacta
     2521 ttttacaata aattccatat aactctacca tctatatcct taacattttg acaaccagtc
     2581 aaaatcatag tctcacataa ctcattccta accttctcta taaaaaactc cacaccatca
     2641 agaccaccgc caaaagaagc agtaacaaaa ggtcttccca tcaaaacagc atcagcaccc
     2701 aatccaagca tcttaactac atcaacacca gtcctaactc caccatcaac aagtattgtt
     2761 atctttccct ttacagactt agctatatct ggaagtacct cacaagtacc aggagtataa
     2821 tcaagtactc ttccaccatg atttgaaaca ataatggcac tagcaccaga ctctacagcc
     2881 atcaaagcat catctacagt cataattcct tttataataa aaggtaatct agtggattca
     2941 accaattctc taatctcatc aattgtttta ggagaaaaag gattttcttg aaattgatta
     3001 ttaataagtc cacaagcatc aaggtctaca ccaactgcaa aagcacctgc ttcttcagac
     3061 aatctaattt tatctattat ctttgaatta ttccaaggct ttataaatac aattccattt
     3121 cccctattat ccttcaatac atctaaatta tccagtaaaa aagtatctac attagtatcg
     3181 ccaaccatag cataaattcc agaattagaa caacctctaa caacaggctc tatgtattct
     3241 ttttcggaaa ctttaccacc catgttcaat atagtacctg atacaggtgc agcgaatata
     3301 ggagaactca tttttctacc aaataattct atggatgtat ctggctttga aacattatgt
     3361 atgactctca tatttatctt tattttttct agactttttc tattttcaat gaaagacgaa
     3421 ccacttcctt taccacccat accaggaaca tctccagcac aagctaatcc attacaaatt
     3481 ttacatactt tacatgttcc attaaagttc tctctagctg attttagtaa gtcattgtaa
     3541 tccatacaaa atctctcctt tttataatat taaaattgag tattactata ttgaaaatat
     3601 cttaattgta aatataggga tgttattatg atgagctaaa ctagataata catattagat
     3661 attttttatt gttgtagata gttaaattat atcatattag atagtcaaat tttgaaaaaa
     3721 actaacttat ttaagtatac attttgaaaa aattaaaaaa gctatatata gatttttaaa
     3781 gaaatctata tatagctttt aagtttatgt caatcttttt tgttaatgaa taaataatat
     3841 agaatacaat atacaaaaaa gtttcttgaa atgctaagat aatccccatt tacaaagcaa
     3901 catcattata ctgtgtaaat ttgaatcaac aatgttttca ccatatttca tcataaaatc
     3961 ctctaattgt gtgtcaataa atgaataata tccacttata ctatcgatgt atgcagtcat
     4021 taagtgtata cattcatcac ttttatcttt atacatatat atctccccct atatacaata
     4081 tataaattta gataccaaat cgtatcatat taaatagtta agtgtgtaac atctatgcag
     4141 ttatatattt atatcattat attaataact aaagtgagtt ttgacaatag attttgggaa
     4201 actggatgtt caaagtgcaa acttggattt taaaatcatt aattaagata gcgcatagga
     4261 atatatgtat acataactta attataaaga ttataaacaa ttacttttat taactttatt
     4321 taaatggtag aataacttgg agattaataa ttataaggag gatataatat gagtttatat
     4381 aagtttatat atgatgataa ggaatattta ttaaaagagg agaactgtag tgctttaata
     4441 aatgatgaag aaaatccagt tcaaggagta agtatttcaa aaataattga tatacttaat
     4501 gaagcagaag aagttgattt tgatgtagaa tattatcaag aagcatgtcc attatgttta
     4561 gaaggagtaa aagaaaagaa aaaattcttt ccttttttag aatatcactt ttatattttc
     4621 agtaaagatg gagaatatgt aataagcaat atatctgttg attataaagg actatctttt
     4681 aataaactat caagggcaaa taaagttgat aatagttata ttgtaagtgt gataatttgc
     4741 gaaaattgtc aggattatat agttcagata gaaaattgta tagtttagtg taaagtatgt
     4801 cttaatttaa gagaaatttc taaattgaga catcaacttt tttcaaaaat agtatggaaa
     4861 attactaaat ttatgagata atgtaagtcg gaggtgagtt acaatatata gtaaatattt
     4921 ggaagagata gatgatacaa ttttagattc aatgtttaaa ctaaccttga atgaaagatt
     4981 attgactaat aacgatttta ttgaaaataa aattgatgta gtaaagaatt taattgattg
     5041 ctataaaact gaacaaaata gtttaatgtt attaaaatca aattttttgc tagctttatt
     5101 atatggtatt caagggttta gtgaaaaaat gaaagagtat attttgattt cctgtaaata
     5161 tattgataaa tgcttaccca aagattataa atttttagca cgattttatt cacaaatagc
     5221 tattttaagt ttaaaaaatg aagatgtaaa taaggcaaat ttatatatgg ataaatttaa
     5281 tcttatttgt gatgaaaata attttctaat tgaagaaatt atttttaaat cacaacttat
     5341 ttacattaag gcaagtaagt gtatagaatc ttctgtaata attaaagaga tagaaacgtt
     5401 gtatataaag ataaaagaaa ttaacgattt taattgtaaa aaaatatatt tttttattat
     5461 tggtaaaata tattttttgt ttttggatga tgctctaatt gctaaggtaa attttttaaa
     5521 agcaagaaaa tatgcagatt tactaaagga tatgcaagta tcttcactat gtaatataaa
     5581 gataggtgag tgtgaatgtt cgtttgaaaa ttatgaatgt tccatggaat atttcaatga
     5641 agtaataagc aataaaaagt atagaaatgt aaatataatt caaaaatata gagcatcaaa
     5701 caacataact aaaatattaa taaagactag aaattatcct gtcgcaataa ataatttagc
     5761 aaaatctgaa gtatatctag aaaaaattaa aaatcatagc ttaaaagaag tagagaagtt
     5821 tgatttattt ataagtttag ccatgtatta tgcaaaaagt aatgaaaaat cttttgaaca
     5881 atcaatatgc tatttagata gagctaaaaa catattaaat atgataaaaa atatagaaga
     5941 atatataata tatgatttag aaataattta tcaccaggca aagatatatt atatctttgg
     6001 atattatgat aatgccttaa acacaaataa aaaactcctt gaaaaatcta aaaatgctaa
     6061 aaattataat tatgtgaagt tagcatacaa gagtatatat ttgtgttttg aaaagattca
     6121 aaattatgaa atgagtatga aatactttaa aatgtattat gagttaaaaa tgatgtatat
     6181 aaaagctcga aatagaaatt atatagattc cttaaattat agacatgagt atatagaaaa
     6241 agaaataaat aacatgagaa aagtaaaact tgatttagat aaaaaaaagt atgttgacca
     6301 tttgacaagt gcatataata gaactttttt gaacgatttt ttagaaaaac aagaagttag
     6361 tgaatattgc acagcattta tgatagatgt agattacttt aaagaataca atgatactta
     6421 tggacactat aatggagata ttgctcttaa aaatataact ataataataa aaaaatatct
     6481 aaaaaaagac atgcttatga taaggtatgg tggagaagaa tttctgatat tatctgtatg
     6541 taaagattat aaaaaaagta aaatatttgg aagaaaatta tgtaggattg taaagaaatt
     6601 tttaaacaat aatttgacag ttagtattgg cattgataca tgtaaaaata gtagtataga
     6661 tataaaagaa ataattgaaa atgctgataa agctttatat aaagcaaagg aaagtggtag
     6721 aaatagatgt ttgcattatc atgattttaa gaatttttaa ctgttgaaat aggattggtg
     6781 atttacttga aaaggagttt aatagaagat ttaaagagaa atataaacaa gatagaatac
     6841 gatatgttga ctaaattcta tctaaatttg acagatttaa actacatctt ggattatcac
     6901 aaagaccttt taaatacaaa attagaagat aatagtagag tctatacttt atatttaatt
     6961 agtcttataa actatataaa aaaagataga gaagcatcat acaactattt gatagagtca
     7021 ttaaagtata ttaaaaaatt gtcattaaaa gatgattcag atataatatc taaaatttat
     7081 atttatctta ctttatcagc tatttctata agaaaatatg ataaggtaaa taatttttat
     7141 ttctcagcaa aaaaaattat aagacaaaat catttaaatg agttgttggt attgttaaat
     7201 atgagtttat gtgtagaaat aagtgcttta tatcagacca aaacggatgt aatagcttta
     7261 gtagaagaag tgtctttttt taaaacttta aaaaataaag ctcttgtaag tagggcaaat
     7321 tatatatttg ggagagttta tctatattta tttaataact ttataaaaag tatggaatac
     7381 ttaattgaat gtctaaaatt ggctcaagaa aataatttat actctataga ggctttttgt
     7441 aaatgtataa taagtttgtg ttatttagag agtggaaata atgtagaagc tataaaatac
     7501 ataaataatg tattagatgt tgcacacagt aataactcaa tatcagtaac tgaaagagtg
     7561 tctataaaaa tagaccttat atgggcatat ttaaaagaag atatgaataa agaagcagaa
     7621 gttattttat taaagactat aaaattaatg aaagttgttg aaggtgtata taaggattat
     7681 ttatattcat ttatattttt atattcagct gaaattgagt taaagaaaga taattgtagt
     7741 tttgaaaagg taaacaccta tttaatttta tcaaaaaata tatataaaag ttttggagat
     7801 gtttttatat ataattctaa actagattac atatataagt tgtatggaga tttatatata
     7861 aaatttggta atatagatga aggtataagt tgttatataa aaggtttaaa tttagttaaa
     7921 aagagttcat tgaatcttag aaaaatatct atattttatg ggctaatagc aaatggatat
     7981 gagctaaaaa aagattttaa actggcaata gaatattata aaagtatgga ttggtattct
     8041 gataggtggg aaaaaaataa ttcctataaa acatctcaag caattcacaa acaatatgaa
     8101 ctcagacaaa aacaagagag tttaagatta ctagtggatg ataataagaa attagaaaat
     8161 gatattttaa aagatggact tactaaacta tataatagaa gatatctgga acagcagttg
     8221 gatttattta atgaaaccag agacaaaaat atggtcgcaa tactggtcga tatagattgt
     8281 tttaaaagtt ataatgataa ttatggtcat ctagcaggag ataaagttat agttaaggta
     8341 actgatatta taagaagtgt atttatagat attaccgaac acataatcag atatggggga
     8401 gaagaaattt taatattagt agattctata gaatcaatcg gtgttgaaga gattgataat
     8461 atagaattat atattaaaag agttttcaaa cttcttgaat ttgagggtat tgaacatata
     8521 tattctaaaa taaaaaacta tataactata agtgctggag taagtataaa aagatgtcgt
     8581 agtagaaaag atgttgaaat attaattgaa gatgcagata agaacctata taaatcaaaa
     8641 aaagcaggta gaaatcaata cattataatt taaaattgag gtgattttgt tgcgaaaaaa
     8701 aataggaatt atagcctctg atattgaatt aaaagaaaga atagaagaat tatatagaga
     8761 agatgtagaa aatggaacta taataatcga catacttaac ctagacttaa tggaaaatca
     8821 aggtcgtata cttgttgaaa aaggtgccca agctataatt ggtagaggtg gaggttatag
     8881 tttagtaata gatacagtaa atgtacctgt tataccaatg aatatgaagt caactgacct
     8941 tttaagagca atagaaatag caaagaaata ttctaaaaaa gtagtattga ttcttggaga
     9001 caatgaagtt tcttttgact atgttggctg gagaaatgtc ataagtactg aaataactga
     9061 agaatggttt gaatctaagt atgagataag aagtaaagtt gtaaaatata tagaccaaaa
     9121 agatgaaatt gtaatcgtag gaggaggttt ggcatgttct tttgcaaggc aatatggaat
     9181 tgatagtgta tttgcaactg ctagtgatga atctataaga gaagctgtag aatattgtaa
     9241 aaaattatta gatacattag gtgaagaaaa gtttaataat gaagtgttac gaaatatact
     9301 tgatggtata aaagatggag taattgctat agatagtaat ggcagtataa ttttgtacaa
     9361 tgaaagtgca aaaaacatgc ttaaagtaga gagaaaatgt gcacttaata aatacatctt
     9421 agatgtattt cctaaaatgg agtggatgtt agattgttta catgaaaaag aggacgtaga
     9481 agatagaaag ataagaaata taaataatct aattgttaat actaggacaa cattgataaa
     9541 agttgataat agtacttatg gagttttagg aataatacaa gatataacca agctacaaaa
     9601 cttagaaaga aaaataagat ttgacttgaa tcaaaaaggt ctctatgcta gatatacttt
     9661 tgatgatttt ttatttaaag ataaattaac taaagaattt attgaagaag ctaaaaaaat
     9721 tggaaaaagt gattatacaa ctttacttta tggggaaagt gggtctggta aagaaattat
     9781 agctcatagt atacacaata taagtaaaag aaaagatagg ccttttgttg ctataaattg
     9841 tgctactata gcagaaaatt tacttgaaag tgaacttttt ggctatgaag aaggtgcatt
     9901 tactggtgct agaaagggtg gaaaaagagg gctatttgaa cttgctcatg gagggactct
     9961 ttttttagat gaaataaata gtctatcctt taatattcag acaaaactat taagagtaat
    10021 agaggaacgt caaataatga gaattggttc tgattatata atacctctgg atataaggat
    10081 tatagcagca acgaacgaat ctttaaccga aaaaatagtt atgggtacat ttagagcaga
    10141 cttattttat agattgagta gtttagagat aaatatacct cctttaagag acagaagaga
    10201 agatataata cctttgttta acaattttgt aaatgaagta ttaaaggatg atggtctcaa
    10261 tggaataaac agtatagatg aaaattttgt tttgactaaa gatgaaatgg ataagctata
    10321 taattatagc tggcctggaa atgtaagaga gttaaagaca atagctcaaa aatatgttgt
    10381 gacaggtaaa attaaattga gacaagatag agattttaaa actaaaaaat tacttcctaa
    10441 ctctgaagta gacaaattta attctgaaac gacagcaagt gtagaagtcc aagatgaatc
    10501 tataaatata agtaagataa atgatggtaa gataagtata gatataaaag aagtaaataa
    10561 gtatgttgaa gaaaaaataa taagtatgct ttttgctcaa ggactttcta aaaatgaagt
    10621 tgcacaagtt cttggaataa gcaggacatc tttatggaag aaatacaata aaaatattta
    10681 aatataatta catataaatt attttgaagt aatgttaatt ttttaaaaca agtgttaaga
    10741 aaaattaaca ttactttttt gcaaattttt atttttttag acaggcatat atatttataa
    10801 gaaaaatagt taaaacactt aatttaataa tgaaaaaaat aataattcta aacaaaatca
    10861 attttacata aagtgggatt tattggcata gtaattgctt aatatataat gtaagagtaa
    10921 atacaatttt aggggtgatg gtatgagtca aagtatggtt ggtaagcatg caatgtggcc
    10981 aaaagaaaat gatgttatat ttagcatatc tggcagagct caagcagcag agaaggcttt
    11041 tggtatggat aatgtaataa atgcaacaat aggcgctcta atggatgatt caggaaaatt
    11101 gataacaatg aaaactgtat atgaagaata taaagcatta gataactgtg agataggtgc
    11161 atatgcagct cttgaaggtc aaccagatta cttagaagca gttaaaaaag tatttttcag
    11221 agattactta ccagaaggac atataagagt attagcttca ccaggaggaa gtggtgcaat
    11281 aaaattagct gtatggaact atactaatga aggagatgag gtattaacat ctgattggtt
    11341 ctggagtcca tatgtaagta tagctgaaga ggctaataga aaagtagtta attaccaact
    11401 ttttgatgaa aatagaaggt ttaattttga gtcgtttaaa gaaaaatttg taaatatagc
    11461 agaaaaacaa ggtagagtct ttacaattat aaacacacca gcgcataatc caactggata
    11521 tagtgttgca gatgatgagt gggataaaat attagactta tcaaaagaag tcgcaaaaga
    11581 taaagacaaa aagataatat ttttcgtaga ttcagcatat attgactttg caggtgatga
    11641 tgatgtatgt agaaagtttt ttaagaaatt ttctaatttg cctgaaaacg ttctcgtatt
    11701 ggttggattc agtatgtcaa aaggatttac agcttatggt atgagaatgg gggctataat
    11761 ttgtatatct tcaagtgaag atgtagctga agagttccat tactcatgtg ttcattcatg
    11821 tagagcaaat tggtcaaatt gtaatagaag tgccatggca gtattatcta acattgtaaa
    11881 tgaccctaaa aaattcaaag aatatgagga tgaaaaagaa atatacaaaa acatgcttac
    11941 tagaagagca gatgtatttg taaaagaagc tgaaagagta ggattagaaa ttcttccata
    12001 tatagcaggt ttctttgtaa gtataccatg tgataatcca aaagaagtat gtgaagaatt
    12061 gactaaacat aatttatttg cagttccatt aaaaatgggt ttaagatttg cagtttgtgc
    12121 agtttctgaa gataaatgta agaaagcacc aagtattata aaagaagcac ttgaaagttt
    12181 agaagtaaaa ataaataatt aatttcattg aacaaattgg gtggactatg aatttaataa
    12241 ttgtagatgg agggaatttt atatgaaaca attgatgaca ggtaatgaag caattgcacg
    12301 tggagcctat gaggctggtg ttaaatatgc atctgcatat ccagggactc caagtacaga
    12361 aatattagaa aatatagcaa catataaaga tgcaatagtt gcagagtggg ctccaaacga
    12421 aaaggtagca cttgaggctg ctataggggg ttcaattgca ggagcaagaa caatggcttc
    12481 tatgaaacat gttggtttga atgttgcagc tgatccaata tttacttatg catatacagg
    12541 ggtaaatggt ggtatggtac ttattacagc agatgagccg ggaatgcatt catctcaaaa
    12601 tgagcaagac aataggatgt atgcaaaatt tgcaaagata cctttatttg agccatcaac
    12661 tagtcaagaa gctaaagata tgataaaaga agcttttgaa gttagtgaaa aatacgatac
    12721 accagtttta tatagagtta caacaagact ttgtcattca aaaggattag tggagtgcta
    12781 tgatagagaa gaagttgaaa ttaaagagta tgttaaaaat gctaagaaaa tggtaactgt
    12841 tccagcaaat gctcaaataa gaaggggcgt tgttgaagaa agaatggaaa ttcttaaaaa
    12901 attctcaaat gaaactgatt taaactacta tgaaataaat gatacaaaaa taggtgttat
    12961 agcctctggt atgtgctgta attttgccaa agaagttttt ggaaaaaatg catcatatat
    13021 gaaactagga tttacaaatc cattacctta tgaaaaaata aaagaatttg cagaaaaagt
    13081 tgataaaata tatgttatag aagaaaatga cccatttata gaagaacaaa taaaagcata
    13141 tggaatagat tgtattggaa aagatgtaat accaccatat ggagaaatga caccagatgt
    13201 tttaagaaaa gctatatttg gaaaaacaaa tgatactata gaatacaaat ctgaattagt
    13261 aacaccaaga ccaccaagtt tctgtgcagg atgtccacat agaggattct tctatgaact
    13321 tggaaagaga aaaaatttaa tagttggtgg agatataggt tgttatactc ttggatttgc
    13381 tcctccatat aatggaatag attatgttgt ttgtatgggt tctgcttttg gaactgcaca
    13441 tggagctcaa aaagttctta atatgaagga tgataatgaa aaaagattgg taggtgtact
    13501 tggagactca acattcttcc atacaggaat aaatggactt ttagatgttg tttacaatag
    13561 aggtaattct atttcggtta tattagataa tagaataact ggaatgacag gtcaccaaga
    13621 aaacccaggt tcaggatata ctttacaagg tgctaaaact aaagaagttg atatagaagg
    13681 attagttaaa gcttgtggaa tagaacatgt aagagttata aaccctaata atttaaaaga
    13741 agtaaatgaa gcactagatt gggctttagc tatagaagat gaaccatcag taataataac
    13801 aagatggcca tgtgtactta aaaaattctc taaagaagat atagaagaat ttaataatcc
    13861 atttaagact aagtgtaaag ttgaccatga taaatgtata ggatgtaaat tatgtttgaa
    13921 aactggatgt ccagctctat catttgataa agagaataag ttatcaaata tagatagaaa
    13981 tcagtgtgta ggatgtggag tatgtgcaca agtttgtcca aaacaagcaa tagttaagga
    14041 ggaaaaataa tatgactaag agtatacttt tggtaggtgt aggaggtcaa ggaactatac
    14101 ttgctagtaa attattaact atgggattga tggaagctgg atatgatgta aagatgagtg
    14161 aaatacatgg aatgagtcaa agaggaggtt cagtttcctc acaagttaga tatggagatt
    14221 gtgttcattc tccagttata gaaattggtg gagcagatat attagtatct tttgaaaaaa
    14281 tggaagcttt aagatggttc aattatttaa aaccagaagg aaaagcttta gtaaataatc
    14341 ataggataga ttctatgact gttttaatcg gaggagctga atatcaagaa agtgatatag
    14401 atgcagagtt aagtagatta aatgcaaaag ttataaatgc agctgataaa gctgaagaac
    14461 taggaaattc aaaaataatg aatgttattt tattaggatg tctagttaaa tctatgcaat
    14521 tagagtctat tgattgggaa aaaattataa gtgaaaatgt taaaccaaaa tttgttgaat
    14581 taaacataaa agctttccat gaaggtatgg aaatggttag caaatagttt ataatagata
    14641 taaatagact atagaaatta aggtgaaagg aagaaaatta tgacttacag aatattagcc
    14701 ataaatccag gttctacttc tacaaaaata gcagtatatg atggagaaga acaaattctt
    14761 gtgaagacga tagaccatcc agctgaagag attgcaaaat ataatactat acaagaccag
    14821 tttgaaatgc gtaaggaagc agttttgaat attcttaaag aaaatagtat agacttaaaa
    14881 tctcttagtg caatagtagg aagaggtgga gttttaccac cagtaaaatc aggagcatat
    14941 ttagtaaatg aagaaatgat tgatgtacta agacatagac cagtacttga acacgcttcc
    15001 aatttaggtg ctgttgtggc acatgcaata tcagaacctc ttggaatcaa ctcatatatt
    15061 tatgattctg ttgcagtaga tgagcttata gatgtagcga gaatatctgg actttgtgga
    15121 atggatagat caagtgcagg gcatgcatta aatactagag caatggcttt aaaatatgct
    15181 aaggataaag gaaaagatta taagagctta aacttaatag tagctcacat tggtggagga
    15241 gtaagtattt atcttcatga aaaaggaaga atggttgata tgctatctga tgatgaagga
    15301 ccattttctc cagaaaggtc aggaagagta cctgctacaa aattagtggc tgcctgttat
    15361 tcaggtcaat attcagaaag agaaatgact aaaaagataa gaggtaaagg tggtatagtt
    15421 tcatacctaa atactgtaga tgctagagaa gttgaaaaaa tgatagcaga aggaaatgaa
    15481 gaagcaaaaa ttatttatga agcaatggct tatcagttag caaaaggtat tggagagtta
    15541 gcaactgtag tagatggaaa ggtagatgct ataattataa caggtggaat tgcatattct
    15601 gaaatgttta cttcaatggt taaaaagaaa gttgagttta tagcaccagt agaaattatg
    15661 gcaggagaaa atgagttgga atcacttgct tttggaactt taagagtact aaatggagaa
    15721 gaagaagcta gaatttatag tgaaaattaa taagaaaaac taaaaatgtt tatatactaa
    15781 aaaagtggta tatccgtttt ttatacacga aagcttagag tataggtgaa aatgtataaa
    15841 atttggattg ccacttttta taattaaaat tttatcttaa cctaataaat aattctgtaa
    15901 aatttcgtat ttatatatat attttattgg tatttattgt tattattatt atgaggagtg
    15961 atttttgatg ggaaaaataa gtttaaaaag tatgataaga ttattttcag gtttttttat
    16021 atttgcaatt tcttctgtgc ttatgattaa tgcacatgta ggtcttatgc cttgggatgt
    16081 acttcaccaa ggtttatcta taaagctagg cataactata ggtcaggctt ctataatggt
    16141 aggagttgta attgttatat tggatgcagt ttttggtgaa aatataggat ggggtacttt
    16201 actcaacatg acatttatag gaatttttat tgatttagta atattttctg gagtgatacc
    16261 tcatgcaagc aacacttata taggtgtgtt tatggttgtt ataggaatta ttttggcagc
    16321 tatagcaagt tttctatact taggtgtttg tttaggaagt ggaccgagag atgggcttat
    16381 gattgctctt cagaaaaaaa ctaataaatc tgtacgtctt gttagaacaa ttcttgaaat
    16441 actagcatta gttgttggat ggttattagg aggttctgta ggtattggta cgcttgtaag
    16501 tgctttaggt ttaggatatg tattacaaat agtgtttaga atatttaaat ttgacacaaa
    16561 attgctgaaa catagattta ttatagatga tataagagaa tggaaggaaa agaaaagcaa
    16621 tgaacataaa tgtaaatcaa gtattgtaat aaaaaatgag cagaattaat tatgctaaaa
    16681 tatatttgta gtttatctaa tttatagtaa agtcaaggag attttgaaat ggaattatgg
    16741 gatttatata atgctgatgg aattaaaaca ggaaatgtta taaaaagagg taattctatt
    16801 gaagaaggtt attaccattt ggcagttgaa gtgtggattt taaatagtaa ctctcaaatt
    16861 ttaattcaga aaaggtctaa aagtaaaaaa acattgccaa atatgtgggg aatgacaact
    16921 ggatgtattg tttctgggga agacagttta gaaggagcaa ttcgcgaagc aaaggaagaa
    16981 atagggatag atataactaa agatgagatg aaagttttta gaagtatgat tcatgaagat
    17041 actctttggg atgtatactt agttaaaaaa gaatatgata tatctaaagc tattttacag
    17101 gaggaagaag taagtgatat aaagtgggta agcacagatg aaataagaca attactaaaa
    17161 gagggtttgt tttttgaata tccagagata tacgaattgc tatatgagat agataataat
    17221 aaattaaata tatgatgtag agcagatttc agtataagta agattattta aaatgtatct
    17281 tctctaaatt ttaatattgt tttatgaaaa ttaataagtt atttttagta tttttctact
    17341 tgttttttga gattaaaata aaaagggcag tcattataaa gtcataaaaa tttggggagg
    17401 atttaaacta tggaagataa caaaggattg gcacgtgaca gttcaaaata taaaattgtt
    17461 tctatatctg tcgtagtaat tattctaatt acattttggt atatgttaaa tatggtgctt
    17521 ttaacattta ttatgacttt tattttctat aacttgcttg tagccacaag aaaacgaatt
    17581 aagaagtttt cttctttaaa tattccagat tcattgataa ttatagtgtt atatgcacta
    17641 tttgctatat tgttagtatt gattagctat gcagttgtac caataattat agttcaactt
    17701 acagagttga gtagggtttt tagtgatttt gatgtaaatc aatttgctca atctttagga
    17761 ccgaaattgt atcctattgt ctcaaagctg gattttaata aatatatttc acaagcaggg
    17821 ctacttatag catcaacagc aacaaaagtt ggaagttttg gagtaaatat attattggca
    17881 tttttactga gtttactcct tttacttgaa aaaaatgaaa ttaaaaattt tggagataaa
    17941 ctttctgata gtaagatttc ttttatttat aattcgcttg tattttttgg aaagagcttt
    18001 gttaaaaatt ttggtgaagt aatgaaagta caagttatga ttgcctttat aaattctgtt
    18061 gtttctatga ttttcttagg tttcatggga ttccctcaaa tatgggcttt aggatttatg
    18121 atttttgtgt tgggtcttat tccagtagca ggagttatag tgtctttgat tccattaact
    18181 gtaatagcat ttaatacagg aggaataaca aaggtttttg gagttttact tatgatttgt
    18241 atagtgcatg cagtagaaac atacatatta aaccctaagt tgatgtcgaa tagaactaaa
    18301 cttccagttt gttttgtttt tattatttta cttgtaggag agcattattt aggtgtatgg
    18361 ggattactaa ttggagttcc aatatttatg ttccttatgg atattctggg tgttaaattt
    18421 acttctaggt aatatattaa aataataatt tatttttata tataaattat gaattgtttt
    18481 aaaaaagaac ctataaatag acatcattaa taaagacatc tatttgatag gttcaatttt
    18541 gctttgtata aatactataa aactaaataa aaagaaattt atttcatttc aaaagtttca
    18601 caacttgcat ttctttcatt tatatgaact ttttcagctt tgcaaagttc atttttattg
    18661 tatttacatt ttacagcttc acaatgaatg ttatctgtat caacttggtt agtttcatta
    18721 gaacgactag ttataccaga tgtatatttg tcttcaaaag aagcacaata agtattttct
    18781 gtagtagttg cttgcattcc atctactttt atactaccag cataacaata tccactagta
    18841 ttataagcac aattagtagc agcacaattt aaattattat ttttcataaa taacctccta
    18901 agttttattt aaacttatta tgtgaagtta tctaaatttt atacacttat ttacaattac
    18961 atttatcaca tttaacaact acatctttgg aagtttccct tcctaaattt ttattaacat
    19021 gatacacatc aacattttca gttgatccac atatagaaca tttaaaattg ttttctagta
    19081 gggttctttc tttaaataac ttccaataca tagaattgat gtaatcatta taatttacct
    19141 tgaggcatgt ctcaagaaag tttatgtata gattgtctaa atctcctgta tttaattctg
    19201 tatattctgg ttcaaaatat tcacaacatc ctctaaaatc gggctttgta tctgaaataa
    19261 cacatctaat atcatatcta tagtctaggc taggcccctt aaatgaaaga tgtgtgcaaa
    19321 aagaacaaga tatttctggt agattttttt caagttcatg taatttatta ttcataagta
    19381 ttcctccaaa aaatttattt taagtatata aatgaataga ttattttaaa acttttattt
    19441 atttatatta gagatttagg ataaatattc caataaggag taaatactta agtatttatt
    19501 actgtattaa ataaaaaaga ggcaaatcta acttgagatt gcctactttt ttatcatttt
    19561 ttaataaaat ttattctaag tattatttta tttagattta gtcagccaac ttagtatctg
    19621 ctttgctttt taactttcta ccagagcgta cagttaaaat agcatataat acagtaaata
    19681 tggcagctaa aacatatgat atatttatgt ttaagttaaa tccaatttga gcattaaata
    19741 tataactgaa tataacaaag gaataaaaca taccaggaat taagcttata atataattct
    19801 tttcttttat tataaggtaa actgtaatca ttgcaaatgc aaatatggca atagtttgat
    19861 tactccaaga gaagtatctc cacaatatat taaatccaga agcactaagc tttgcaaata
    19921 taaggatggc tatgacagga ataaatatac agattgacaa tataacacga tgtttctttt
    19981 ccttttggtc ataatgaaag taatctgcaa gcattaatct taaagatctt aaagctgtat
    20041 ctccagaagt tattggtaaa acaattacac ctattatagc tatgatacca ccaatagaac
    20101 caagtaaatc tcttgcaacc aaacctatta catcaggaga acctactaat tctttaggta
    20161 ttcctttatt gtaaataccc atagcagctg cagcccaaat cattgcaatt aaaccttcta
    20221 aaatcatcat attataaaaa gtagtttttc cttctttttc atttgaaact gatcttgcta
    20281 taagagtagc ttgagttgag tggaatccag aaacaatacc acaagcaact gtaacaaaga
    20341 atgtaggaat aagaggaata ccatcaggat gaataccttt ccaatttgca agacttaaat
    20401 tagctaaatc ataaccttgt gtaaagattc caattccaac accagcagca gataaaagta
    20461 gaatagctcc aaatattgga tatacctttc ctatgatttt atcaattgga aatagtgttg
    20521 cacacaagta atatagaaag ataagtccat aaactatcca gacaactgga ttatttatag
    20581 tcgatttcat atttaaaatt tgagtaacaa ctaaatcccc aggtgtataa ataaaaactg
    20641 caccaactaa aagcattaaa agacaaagga atatattata gacttgaaat actttattac
    20701 ctaaaaatct actgataaga gaaggcattt gtgctccttt ttctcttata ctaatcattc
    20761 cactcatata gtcatgcaaa gctcctccaa atacacagcc gataggtatt agtatgaatg
    20821 ctattggacc aaacaatata ccttggatag gtcctagaat tggtcctgta ccagctatat
    20881 taagcaactc aattagacta tttttccatt ttttcattgg gacatagtct actccatcac
    20941 tttgagcaag tgcaggtgtt tttctgtcat ctgggccaaa tactttttca caataagcac
    21001 catataaagc acctcctatt agtaatatag ccaagccgat taagaatgtt gtcataatat
    21061 caccccattt ataaaatttt ttggtctttg tatacatatt atctaaatgt gtagatttat
    21121 attgaattta attctggaca acctacaaat tctataaagt attacaacta atataatttc
    21181 agaaataaat acatatacat aaccatgtac ttagtataca ggtatttggt aatattttca
    21241 atgttatagc aataaagaat ataaacaaaa ttctgataat attttacaaa aaaattaaaa
    21301 aatataaaaa ttagtttaaa tagatggaga aaaaaggaaa atcaattgag aatataaatg
    21361 tctatatctt aacaatatta agaggtttaa aattcttgaa gaaagaatag tcaagtgata
    21421 taatgggttt acaggtgtat atacactagt acatatagaa agcggtgcaa ttatggataa
    21481 agatttaaca tatcaaatca aagagctaaa gaaagaaaaa aatgcaataa tacttgccca
    21541 tttttatcaa ccaccagaaa tacaagaatt agcagatgca gttggtgact catattattt
    21601 aagtgagatt gctagagatt gtaaagaaga agtggttgta ttttgtggag taaggtttat
    21661 gggagaaagt gctaagatat tatcacctga aaaaactgta ttgatgccag taagtaacgc
    21721 tgggtgtgct atggcagata tggtagatga agaaggcgtg ataaaattaa agcaacagta
    21781 tccaaatgct ttggttgtat gttatataaa ctctacagct aaagttaagg cacattgtga
    21841 tgtttctgtt acatcttcta gtgcaattaa aatactagaa aacatagata ataaagaaat
    21901 tatattcctt ccagataaaa atttaggagg atatattgca gaacaatttc cagataaaaa
    21961 ctttatattt tgggacggat attgtaaata ccataacaat ataagagctg aagaaataat
    22021 tgagttaaaa gataagtata aaaatgcaga agtattagta catccagaat gtaaaaaaga
    22081 aataagagat ttaggagatt atgtaggtag tacatcagga ataataaaat atgctactaa
    22141 tagtaaaaac aaagacttta taatagcaac tgaagaaggt atattacatg aattaaagaa
    22201 aaataatcct aataaaaact tttatattcc tggtggaaaa atactatgta cagatatgaa
    22261 aaagacaaca ttagaaaatt tatatagtac acttaaaaat atggaaaatg aagttattgt
    22321 agaagatgaa atcatggaaa aggctttaaa ttcactgctt aacatgcaca aattagcaga
    22381 aggatagagg gatagactta tgaatttaga acaagatgtt ttaatagttg gaagtggagt
    22441 ttcaggatta tactgtgcat taaatttaga taaaagttta aatgtgctag tagtatcgaa
    22501 atcaacaatt gaaaacaaca atacatatct tgcacaaggt ggaatttcaa ctgctagaaa
    22561 tattgatgat atagaatcct ttgtagaaga tacaatgaag gctgggcagt ataaaaatag
    22621 agttgaagca gttcaagtac ttgcagatga atctatagaa aatgtaggtc agatagtaga
    22681 atatggtatg cctttagaca aagaaaatgg agagatagat tacacaagag aaggagcaca
    22741 tagtgtaaat cgaatagttc atagtaagga taatactgga gaagttgttt ttaaaacttt
    22801 attaaaagaa gctaaaacta gagaaaatat aactcttata gaagatgcat atcttttaga
    22861 tatattaaaa gatggcaata aatgtatagg agctagaatt tttaaatcta aaaaagagat
    22921 tcatgttttt tcaaaaatag tagttttagc tacaggtgga attggtgggc tttttaaaaa
    22981 ttctactaat caaagacatc ttacaggtga tggaattgca atagctttaa gaaataatat
    23041 aaaaatagaa aatttagaat atattcaaat acatcctaca gccttttatg aagaaaataa
    23101 tgaaggtagg agaatgctta tatctgaatc attaagagga gagggagcta aacttttaaa
    23161 taaaaataaa gaaagatttg tggatgaact tcttcctaga gatgtagttt caaaagctat
    23221 ttttgagcaa atggaaaaag ataaattacc ttatgtgtat ttagatgcaa cacatttaga
    23281 tagtgaatat ttaattaata gattttcttt tatttataat gaatgtttag ctagaggaac
    23341 agacataacg aaagaatgta taaaggtatc tcctgcacaa cattatttta tgggcgggat
    23401 acatgtagat ttggattcaa aaacatctat ggaaaattta tatgcagttg gtgaaattag
    23461 ttgtacaggt gttcatggag ccaatagatt ggcaagtaat tcattacttg aagggcttgt
    23521 attttcaaaa agagcagcta aaaatataaa ctcagttata gataatgtaa aagttaagtt
    23581 tatagatgta ccagatatgg atatagatat agaacaggta aaaaaagaaa ataaaatatt
    23641 agtcataaaa gaaattgaaa gaacaagtga ggattttggc gatgaattat ttgattattg
    23701 atagaatgat acaggatgct ttgatagaag atgttccaag tgaagatata acaactaatt
    23761 ctattgtgga tgaaaattct aaatctactg tagatttaat ttgcaaacaa gatggtataa
    23821 tagctggctt aggtgtattt aagagagttt ttgagatact tggtgatgta gatgtaaaat
    23881 tatataaaaa tgatggtgat agagtaaaga atcgtgaaaa gatagctgta ttaacaggta
    23941 gtactagaaa cttacttgta ggagagcgtg tagcattgaa ttatttacag agaatgagtg
    24001 gtatagctac tattacaagt aggtatgttg aaaagcttga aggtacaaat acaaaattat
    24061 tagatagtag aaaaacgata cctaatctta gaatattaga taagtattct gtcaaagtag
    24121 gtggaggatg caatcataga tttaatttat ctgatggaat attgcttaaa gacaaccata
    24181 ttggggctgc tggtagtgtt aagaaggcag ttgaattagc tagaaaaaat acttcttttg
    24241 ttagaaagat agaagtggaa gtggaaactt tagacatggt aaaagaagca atagaagcca
    24301 atgcagatat aataatgttg gataatatga gtttggaaat ggcaaaacaa gcagtagata
    24361 ttataaatgg aagagccatt gtagaatttt ccggaaatgt aaatttagat actatagaag
    24421 atataggaaa aattggtgtg gatgttgttt ctgtaggagc gcttacacat tctgttaaag
    24481 ctctcgatat aagtatgaag aatttgagaa atatataaat tttatattga aataaactta
    24541 attaaaatag actccttaaa tttgttttat aaatttaagg agtctatttt ttattcatta
    24601 aaattaataa aaatagaaaa aaatcaatca ataatttaat aaaaatagat aaatactata
    24661 tacactttta ataatatagt taatatctat tttacagata aataagaata aacttataat
    24721 ataattgtat ctgtaaaatt ttgtaaaata taaaaagtat agggggaata aaaatgaaag
    24781 tagcttatat ttttagtagt actaatacac ataaaattct agacaaaatg ataataccac
    24841 aacttgaaca aggaactcat ggagtagacg ttctagggat gtttttcttt atggataata
    24901 ccttattttt atctaaaggt aatcctgtag gcgagagact aagtaaatta catgaaaaaa
    24961 ctgatatgat aataatggct tgtgaccaat gtgctataga aagaggtata gagaataatt
    25021 tggttgatgg agctacaatt ggatgtttcc caaacctata tgcttgttta ggcagtgcag
    25081 gtgtggacca agttataacg ttataaatta gtataggata caatccatag aaaaatttat
    25141 tttctgtgga ttattttaag taaaagataa attgtttaaa gtaggggatg aacaaatgtc
    25201 taaaatttta gttgaattta taaacacttg accatcttgt gatgaacatg gagtgtttat
    25261 aaaaaaaatg tcagaaaagt atgctgataa attggaaata aaattatatc aagcagggaa
    25321 agatttttcg tatataaaaa aatatgggat tataacaaaa ggaactttaa taataaatca
    25381 gaaaaagaaa tatgatagat taaataaaga tacaattgaa agggcaatag tagaagctat
    25441 aaacaataat taaaagttta aaaattagga gtatcaaaaa tgaatatatt agaaattgtt
    25501 ttaaatggag ttaaagatac tatagtagct tctatagtta cactgaatgg agcatcagga
    25561 tggcttatga taagttttat cttggcaggt gtacttcaca atgttttaac tccaagaagg
    25621 tttcaaaaac aacttggaaa taagaaattt tcttctttaa ttaaaagtac tatctcagga
    25681 atgtttttac caatatgtag ttgtggagta ataccacttg gaataagtat gtattattca
    25741 ggagcatatt taggaccagt acttgctttt atgacatcaa ctccaataat aaatccaata
    25801 gctgtagttc tttgtctagg tcttttaggt cctaaagtta caatcattta tctaatagct
    25861 ggttttttag taccattttt agtaggattt ttaggaaatt gttttggaaa agaagaatta
    25921 tcacttcata atgattcaga ggaagaagta atagaattag aagaagaaaa aagaagtttt
    25981 atggaaaata tgattgatgg tcttaaatgg tcttttggtg aattagccct tactattagt
    26041 aaatatgtag taatgggtat ggtttttgca ggatttataa caactatttt tccaaatagt
    26101 ataattcaaa aatatttagg aaatccaggt ttgctatctc ttggaagtat agcaatactt
    26161 gcatgtgtta tgtatgtatg tgctgtagga catataccgt ttatagcagc ccttgttgca
    26221 agtggagcat ctcctggtat agcgataaca tttctaatag ctggagcagc tacaaatcta
    26281 ccagaactta taagtatgtt taaacttatt ggaaaaagaa ctgttattat atattctgta
    26341 actttgacac tgtcttcaat aataattgga tatataacaa atttactttt agtgaataat
    26401 aactcagtaa atttagatgc aacaaattct tcaatacagt tggcaaataa actaatgtta
    26461 aatatacctg atagttttga gtatgtatgt actattttaa ttattttatt tgcatttaaa
    26521 tcaataatac caaagatgaa agaggtcata ggaaatcatg cttaaaaaga tagttataat
    26581 atcctgcata gtagttgtgt taatcatttt atcaaaaata gttgatgata acatcaaaga
    26641 agatgcttct attccaaatg tgaataaaga aacattagaa tattttagaa aaaattataa
    26701 agaagacata ataacttgtg ctgaagaaga cttaaataat gatgggaaaa aagatttagt
    26761 agttatatat aaaaaaagca ataattcaaa tgaaatggta gtcgtagttt ctgataaaaa
    26821 tagccactat ataacaaagc caataccagc acctatagag aatcaaacta taacctttaa
    26881 aaatattgat gataaagcac caatagaagt aattgtatca gggtctaaaa atggtaatgt
    26941 aggatatgct atttacagag tagagggtaa aaaatttgtt gatttatttg gcgaagatat
    27001 ggacaaatgt tgctaaaaat aaaaataata cataaaggtg ggaaatttat atgaaattat
    27061 ctaagaagat tcaagcatta atcttatttg gtgtaatggg tacatctata ttaacaggat
    27121 gtagtagtaa accaaaagaa aaagaagaag caaaaactgg aaaagatgat tatacaataa
    27181 cagtaggtta ttataattgt gaccatatga ctgcgggacc agtagcagaa tcggcaggaa
    27241 tatataaaga tttaggtctt aatgtaaaaa ctgttggaaa tggtaaagtt cctgaagcta
    27301 tggcagcagg aaaaatggat gcaggttaca taggaactaa ggggttagtt ggagcaatac
    27361 ctaagggttc accaataaca atagcagcta acaaccatac tggtggttct gaatatttaa
    27421 tagtttcaaa agatataaaa gaacctaaag atttaatagg taaaaaaatt gcaacagata
    27481 tgagtgattt cttatggaca agtgattatg gtccagaaac aggtctacca actgacccat
    27541 caaaatatga agttgtaaat atggattcag acaaagataa atatttagct cttaaaactg
    27601 ggaaaattca agcttttaca tcttgtgacc cttggggttc tgtagctgaa aatgacggtg
    27661 caggaaaaat aatagcatct actcaatata aagaaaaagc taatggcaaa gaatataact
    27721 gttgttcatt ctcattaaac aaaaacttta taaaagaaca tccagattta gcaaaaaaat
    27781 tggttctagc acatacaaaa gcaatagaat atatatatac aaatccagct gaagcagcta
    27841 aaatatttgc aaaatattat aatgtagaag aagaagttgc acttagaact atctataaga
    27901 agactgttgg agaaggtaga actcttactt ggaaagttac aggtgaagaa tataaaaata
    27961 atttacaaat gtataaagat ttaaaggctt tagatgatat acctaaatat gaagatacga
    28021 ttgatacaag cttattggac tcttgtggag ctgatgattt tgacaaattt ataaaagaaa
    28081 aagttaaccc agagtttcct gaaggtatga gttatgaaga ctggaaagca aaggtattat
    28141 aaataaatgg agaatatgaa aaatataata atacttttta tcattatttt gggaagtata
    28201 tttataatat tttttaattt tgaagataaa gaagaaataa aaggaaaatt aagaataggg
    28261 gtatctgatg atacctctgg ttttgtaata aattatatgg taaatagaga ctactttgaa
    28321 aatataaaag taagtgatat aatggatatt tttacaatta atgattgctg agcaagtaca
    28381 atgcagtggg ctctgagttc agaggaaata gatatagcaa tcatatgtaa agatgctgca
    28441 aaacaatatg taaatgtaaa tagtggattt gaaattgttg gtactgtagt tcaaaattct
    28501 gatatatttc tattaagtga taataatcct gaaaagatag gagtcattca aaatagagat
    28561 taccagtcag aattggttaa aaaatactac aagaatgtgg aagtagctcc cttattaggg
    28621 actgcactag catatggatt agaaagtaat ttagtagatg gtgttgtaat agattgcata
    28681 aagtcaattg ggttagaggg caaaagaaaa tctacaacta aattaggaga ttatgatacc
    28741 tatgtattag ttgtaagtaa agagtttaaa aaaagtaaag catatagtga ttttataaca
    28801 ctttacaata aaagtgttga tgctttaaag aataaagcta catttaagaa agcattaaaa
    28861 gagtacagga atataagaaa tatagatatg gaggaaataa gcaattggaa attaaaattt
    28921 ctaaagataa aagcgataga ttaactaaga aaaaaactga tggtagtatg gatattttat
    28981 ataaaataat actattagtt attatatttt tagtctggca ttttgcagca aaagatatag
    29041 ggagttcttt gttacttcca atgccagtag atgtaataaa aggtttcttt ttctgtgtga
    29101 cagatgcaga gacagtcact aatcttttta taacattgca gagagtactt aaaggtttta
    29161 tgtatgcatt attgtttggg cttccaatag gatttattat gggattttct aaaacttttg
    29221 aaagggtttt atctccagta gtagattctg taaggcaagt tccaataatg gcatgggttc
    29281 ctcttactat agtatggttt ggtataggag atggtccaac aatattttta atagcttttt
    29341 caggggtttt ccctataatt ttaaatacta ttcaaggagt aagagcaata tcaaaggatt
    29401 attataatgc agctagaagt atgggagcat cgccaattgt gatatttaca aatgtaattg
    29461 ttcccgcatc tcttccagat atactaactg gttcaaggat tgctataagt acaggatgga
    29521 tgtctgttat ctgagccgag tttatagcta cgagtgccgg tctcggttac tcaatggtac
    29581 aagctcaaac attaatgcat acagatgtat taattgggct tatgatattt gcagctttaa
    29641 taggatttat aatagatagg gtattaaagt ttataaataa aatcctttgt agatggaggt
    29701 ttgcagatta atgaagttaa gtgtaagaga tataaataag acatttgtaa ataatagagt
    29761 acatacaaag gttttagaag atataagtat ggatatagat gatggacaat ttgtctgtct
    29821 tttaggacca tcaggttgtg gaaaaacaac tttacttact ataataggtg ggtttcaaaa
    29881 atcagaacgt ggagatgttt ttataaatga taaaagagtt aaaaaaccgg gcatagatag
    29941 agcttttata tttcaaaatt atgctttatt tccttggaaa actataagag gtaatgttct
    30001 atttcctatg aaacagcaga aaataccaaa agaaaaaaga gaagaaatgc tagaagaact
    30061 tttggttatg tcagacttga aaggaaaaga aaaactgttt ccgcatcaat tatctggagg
    30121 aatgaaacaa agagtggcca tgataagagc tttagcatgt aatccagagg tattactaat
    30181 ggatgaacca ttaggagctg tagattttca aatgagacaa aatttacagg aagagctaga
    30241 acgaatatgg ataaagaaga aaataactgc attaatggta actcatgatg ttgatgaagc
    30301 tgtatatatg agtgatagag ttatagtaat gtcgagagat aaaggaagaa ttattgatga
    30361 tataaatata gatattccaa gacctagaat tagaggaagc caaaaatatg aagagtacaa
    30421 aaacaaatta actgatactt tatcaaagtg ttatgaagtt taaagaggag tagacatgag
    30481 tcaaatagaa aaatacaata gtttggataa tacacaacag aaaataagaa tctctattat
    30541 ggatatgata atagataaag gttctagtgt tacacttcaa gaagtgactg aatatataag
    30601 taaaaagttg aatatagaaa aagaatatat agaaaggaca ctacaatatt ttatttataa
    30661 aaatataatg gttgttgatg ggaatagtat aaattttata tatcctgtat cagcattgcc
    30721 aacaaatcat aaagtaactt tgagagataa tagaagtttc agtgcaatgt gtgctataga
    30781 tgctatagga acctcttgca cattcaacca agatataaaa ataaactcta tttgtagtgc
    30841 tacaggtaag gaaatagaag taattataaa aaatgaaaag attgagtatg tgaataatcc
    30901 aaacttaaga gtgcttcata taaatcttga taagcatcta aattgggctg ctagttgctg
    30961 aaatataatg aatttcttct ggacgaagag tgattttgat gcttggacaa acgaagcggg
    31021 attatctaat gatgaagata tatattgttt agatattaat gaggcaatag tagaatcata
    31081 taaaatattt aaactcaaac aaaaggtact atcatagata tacatatatg aaaaactaaa
    31141 aatgaccttt agataaaaat gataggtaat atccatacta tataaattag tatagagtag
    31201 ggatgtgaaa agtatttaaa tctaaaggta tatttttagt tttttttaga tgattagata
    31261 gccttattaa gtatatattt atttataacc tttgcaactc cattttcatt attagtatca
    31321 gtaacataat ctgcaatttc ctttatacta ttacgtgcat tccccatagc cacaccaaga
    31381 cctgcatact ttatcatatg ttggtcattt tcttcatcgc caacagcaat agcattttct
    31441 ataggtatat ttagattcgt acataatgtt ttaagagcag ttcctttatt agtagtttta
    31501 cctaaaaatt ctagataaaa aggagcagac cttactatag tatatttatt gaataaattt
    31561 ttaggtagtt gctgtatgat ttcttccaat ctttctggtt catcaactat cataatttta
    31621 cagattttaa tagattcatc aattttatct ttttgtatat atttgacttc tatattattt
    31681 aatttcgctt catgaagtgt atatttgcca ggcgtttcat ttggtgtaat taaatttgtc
    31741 attgtattta tgtgtatttt agtcttatat tttttacaaa cgttattata tataaaatca
    31801 aaatcatcta aactcatttc tacatcatgt atgattttct cacttttaac ttcttgaata
    31861 attgctccat taaagcatat aacataatca ccttcatctg ttaaatttag ttgatttaga
    31921 agttctttta caccaggaag aggtctgcct gttgttataa ctacttttgc cccagccctt
    31981 tttgcctcct gaatggactc aaagacttca ggagtaatcc ttttttgtgt atttaatatt
    32041 gttccatcaa tgtctagtgc gattaatttg tacatatcaa cctccaataa attatatact
    32101 caaatttcct gtgaaaacgt tgacacagct agtgttgaac aaagtttcag tgggaaatat
    32161 ggtttgtttt gtatactatt atatcaagag ataagtaatt tagaaagttt aataatatta
    32221 attttttgat aaaaatatta cttatgtgag atgagaatgt aaaaactttg taatcaataa
    32281 ttccaaaatt cttaattaca aataatggta tcgtttgcat tgttaatata aataaatgta
    32341 tattatatag ttagaagttt gaataaatag actgatggta atttgttata ttagttagtt
    32401 ttgtgttata aaatattgta attccattaa tcatatgtta ttattcaaca tgattataat
    32461 ataaagaata tttgatatat ataataattt ataaaagagt attacatagt acatgtaaat
    32521 ctatatttat aaatacaata aagtaaagtt gtaaaggagt gctttaaatg aagttttcag
    32581 agtttaagta tgaaagaccc aattataata gtatgaaaaa agagtttttg agttgtgtag
    32641 aagatataaa taattctcgt aattataaag agcaacaaaa aaatatacat aagattaatt
    32701 tattaagaaa taaaatagaa acgttgtcaa atatagcttc gataagatat agtacagaca
    32761 catttaataa attctacaaa gaagaaaaaa attattggga tgaatatatg cctttgtatg
    32821 aagaattaaa ttcatatttt tataatgcca ttgtaaattc aaaatttaaa tatgatttga
    32881 taaaagagtt tggagagcaa tttttcacaa tagtagagta ttctttaaaa agtttttcca
    32941 aagaaataat aagtgaattg caggaagaaa ataaattgtg ttcagagtat actagacttt
    33001 tagcttcagc tgagataatg tttgatggga aaataagaaa tttatctggt atggggaaat
    33061 tcatgtattc taagaataga aaaacaagag aattagcaaa caaagcatat tataatttct
    33121 ttgaagaaaa tgaaactaaa tttgatgaca tatttgataa attggttaaa cttagagata
    33181 agatgtctaa aaaattaggt tttgaagatt ttgtagagct tgggtatgtt agaatgatga
    33241 gaagtgacta cagagaatat atgataaaaa atgttagaaa acaagtacta aaatatgttg
    33301 ttccaatggc aaatgagctt tatgaaaaac aagctaaaag aattggtcta gaatatttaa
    33361 gttacataga tgaaggcgtg gaatttttaa caggaaatgc aagtttaaag ggagattcaa
    33421 gatacataat taaaaatggt aaaaggatgt attctgaact gtcaaaagaa acaaatgaat
    33481 tttttgattt tatgttagaa aatgaactta tggatttaga aacgaagaaa ggaaaaggag
    33541 ctggagggta ttgtacttat ataccagatt ataagtcacc attcattttt tctaatttta
    33601 atcaaactgc tgatgatata gatgtattaa cacatgaggc aggacatgca tttcagttgt
    33661 atatgtctag atggattgat atgccagaaa ttaattttcc aacgcttgat agttgtgaaa
    33721 tccattcaat gagtatggaa tttataacat ggccgtggat ggatttattt tttaaagaag
    33781 atacagataa gtacaaattc actcacttat catcttcaat taaatttata ccttatggag
    33841 taattgttga tgaatttcaa cactatattt acaaaaatcc taatgtagat aagtcaaaac
    33901 gaaaagaaat ctggagattt ttagagaaaa aatacttacc tcatagaaaa tatggtgata
    33961 attctttttt agagagaggt tgttggtggt ttaaacaagg acatatattt aaaaatccgt
    34021 tttattatat agattatgta ttagctcaaa tttgtgcgtt acaattttgg aaaaaaatga
    34081 ttcaagatag agatgcagga tggaaagatt atataaatat ctgtaaagtt ggaggaacaa
    34141 aatcattttt agatattgtg agtatgggaa atttgtattc tccatttgat gatggctgta
    34201 ttgagtctat tataggtgat gttaaatctt ggtttgatga aataaatgac agcaaattat
    34261 aaaaaattat gagaatttta ttgtataaaa gtgaaaaatt aaaaaatata taaaaagtga
    34321 ttatttgttg tgaaaatagt cttgttttta actaaattga taaaataaac taaaaaagat
    34381 ttgtacttaa aaaaaaaaga aaatacaaga aatatgcaat atcaacattt ggaaaaaaaa
    34441 gtaaaaatgt atacaaaata catataaaaa aactattgca aattttatag aatgtttata
    34501 tacttaagtc aacagagata gaaaaaagct atttatagaa aacaaaggat aaattgctta
    34561 attttaaatt ttctgaaaaa aataataatc cggatgaaga gtatgggaga ctgctttata
    34621 aaaagcgacc gaagggacaa gtttaatctt acttacccaa aagattagat gaaattctca
    34681 ggtaaaggtg actgtacttg gacggggctc tgaaaatgat tttaacaggg aagacaaatt
    34741 gggagatttg tttttcctgt tttttattta tcaagtaata aataaaagtt tgaaagagag
    34801 taaaaaaatg ataataatta cgaataatcc aaaagtaaaa gaagaagttc aaggcagaga
    34861 agttttgttt aaagacacaa cttatattgg aatcttagaa gcaagtagag atttgataca
    34921 tgaaggttat gaacttttat ctcatccact ctatgggagt gtaaagccaa atgaaacacc
    34981 ttacaggact gttatactaa agaaaggaaa tcgtttggat ataaactctt taaccttaat
    35041 tgaagaagca atcatcactg caagtaaatt tcagaataat aaaaagactc caaaatggac
    35101 agaaagtgtt caagatgatt ttagagtaat tgattatgat atattttata atacaataca
    35161 aagaatgcag tatgaataaa aaaaaattgt taaaaagtta ttaaaacagc ctaaatatta
    35221 aatctaattt aatattaaat aaaatgagaa atttaattgg ggaggataag tcaaaatgga
    35281 aaatgtgtat gacttagtaa taattggttc aggaccagca gggcttgcag caggtcttta
    35341 tggagcaaga gcaaagttaa aaactctaat cctagaaaaa gacaagactg gtggacaaat
    35401 tgtaataaca catgaaattg caaattatcc agggtcagtt ccaaatgcaa caggaccaag
    35461 tttaatagct agaatggttg aacagtgtaa agagtttgga gcagaaatgc taagagacaa
    35521 cattgttgat actgagctag atggagatat aaaagtttta aaaggggaaa aggcagaata
    35581 tagagcaaaa gcagtaataa taggaacagg agcaacacca agaaagattg gttgtcctgg
    35641 agaaaaagaa cttactggta aaggggtttc atattgtgca acttgtgatg cagatttctt
    35701 tgaagatttt gaagtatttg ttgtaggtgg aggagacagt gcattagaag aagcaatgta
    35761 tttaactaaa tttgctagaa aagtaacaat tgtacataga agacaaggat ttagatgtgc
    35821 taagagtgtt gaagaaaaag caaaagctaa tccaaagata gagtttttat tagatacagt
    35881 aattgaagaa atcaaaggtg atggaatatt agaatcagtt gtatttaaaa ataaagttac
    35941 tggagaaact catgaatact ttgcagatga agaagatgga acaatgggag tatttgtgtt
    36001 cgttggatta gatgctcaaa ctgatttatt caaaggtaaa gttgatatgg atgaaaaagg
    36061 ttacataata actgatgaag atatgagaac taacatacca ggcgtatttg cagcaggaga
    36121 ttgtagatct aagactctaa gacaggttgt aactgcaact aatgatggag caattgcatc
    36181 tatagtagct gagaaataca ttgatgaaaa atttgggaac tagaatctgt aaattaagaa
    36241 atagtgtctg taaatttaat gaataatatt taaaattaaa attaatattt aaaattaaaa
    36301 taaaaatttc aagggggaat aagaaaatgt tagatttaga taaggctaca tttgaagaag
    36361 aagttttaaa tgcagaaggt tttgtatttg ttgatttttg gagtgaaggt tgtgagcctt
    36421 gtaaagcttt aatgccagat gttcataaat tagctgagac ttatggggat aaaataaaat
    36481 tctgtaagat ggatacaaca aaagctagaa gattagctat aaaacaaaaa gtattaggtc
    36541 ttccaactat ggctatatac aaagatggag aaaaagtaga cgaagttact aaggatgatg
    36601 ctacagttcc taatatagaa aacatgatta aaaaatatct ttaatatgtt gataaattat
    36661 taacat
//
LOCUS       DAMJWS010000034        36204 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMJWS010000034 DAMJWS010000000
VERSION     DAMJWS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.034"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            32..1144
                     /locus_tag="PC399_001818"
     CDS             32..1144
                     /locus_tag="PC399_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF2592678.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATIITFLCIATFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="PC399_001819"
     CDS             1398..3422
                     /locus_tag="PC399_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2592679.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="PC399_001820"
     CDS             3642..4328
                     /locus_tag="PC399_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF2592680.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="PC399_001821"
     CDS             4423..5478
                     /locus_tag="PC399_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF2592681.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="PC399_001822"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="PC399_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2592682.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="PC399_001823"
     CDS             7195..8901
                     /locus_tag="PC399_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2592683.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="PC399_001824"
     CDS             8923..9996
                     /locus_tag="PC399_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF2592684.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="PC399_001825"
     CDS             10008..10730
                     /locus_tag="PC399_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2592685.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="PC399_001826"
     CDS             10746..11903
                     /locus_tag="PC399_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF2592686.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="PC399_001827"
     CDS             11906..12745
                     /locus_tag="PC399_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2592687.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="PC399_001828"
     CDS             12760..13935
                     /locus_tag="PC399_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF2592688.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="PC399_001829"
     CDS             13964..14713
                     /locus_tag="PC399_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2592689.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="PC399_001830"
     CDS             14735..15643
                     /locus_tag="PC399_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2592690.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="PC399_001831"
     CDS             15691..16443
                     /locus_tag="PC399_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2592691.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16421..17764
                     /locus_tag="PC399_001832"
     CDS             16421..17764
                     /locus_tag="PC399_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF2592692.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKVVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVK
                     VELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            17770..18861
                     /locus_tag="PC399_001833"
     CDS             17770..18861
                     /locus_tag="PC399_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2592693.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="PC399_001834"
     CDS             18945..20858
                     /locus_tag="PC399_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2592694.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKHN
                     VDEIIFSIANIEKRRKKEIIDVCKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="PC399_001835"
     CDS             21057..21764
                     /locus_tag="PC399_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2592695.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22066..24177
                     /locus_tag="PC399_001836"
     CDS             22066..24177
                     /locus_tag="PC399_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF2592696.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24375..25340)
                     /locus_tag="PC399_001837"
     CDS             complement(24375..25340)
                     /locus_tag="PC399_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2592697.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWSKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25625..26581)
                     /locus_tag="PC399_001838"
     CDS             complement(25625..26581)
                     /locus_tag="PC399_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2592698.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GHGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26949..27737
                     /locus_tag="PC399_001839"
     CDS             26949..27737
                     /locus_tag="PC399_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2592699.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27860..28330)
                     /locus_tag="PC399_001840"
     CDS             complement(27860..28330)
                     /locus_tag="PC399_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF2592700.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28469..29113
                     /gene="uppS"
                     /locus_tag="PC399_001841"
     CDS             28469..29113
                     /gene="uppS"
                     /locus_tag="PC399_001841"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF2592701.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29156..30061
                     /locus_tag="PC399_001842"
     CDS             29156..30061
                     /locus_tag="PC399_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2592702.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30376..31923
                     /locus_tag="PC399_001843"
     CDS             30376..31923
                     /locus_tag="PC399_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2592703.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            31926..32324
                     /locus_tag="PC399_001844"
     CDS             31926..32324
                     /locus_tag="PC399_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2592704.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32364..34097
                     /locus_tag="PC399_001845"
     CDS             32364..34097
                     /locus_tag="PC399_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2592705.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            34329..35540
                     /locus_tag="PC399_001846"
     CDS             34329..35540
                     /locus_tag="PC399_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2592706.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            35733..35993
                     /locus_tag="PC399_001847"
     CDS             35733..35993
                     /locus_tag="PC399_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF2592707.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT        14497 a         3787 c         6374 g        11546 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagcat atttactgct
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tagctacatt
      481 tataatagtt gggtactaca tcttagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acctaggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cccattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaat gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aaaacttatg
     2701 tttctgcata ccataataca aatttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaatagaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaaaaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agagtttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag tagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattggtct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaatgt atggatactg ccagcaggag cattattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcgttt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagagtta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gaccgattat tggcaaccac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagcac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttagaatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacac caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atcaaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagatttta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaagc aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ctattaaaag tatagatttt gaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaaaca agttttaaga
     7861 caaaatataa tagatgaata cgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gttttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagcaatga tagaagtttt
     8401 ataatgggat atgaggaaag ctatggttac ttaattggaa ctcatgctag agataaggat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa caatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaacg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcaacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctagtct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtatt atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgatccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcggca tgttttttaa
    11101 tggtagctat attattattt aatttatttc cttcattaat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaatacttt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccgg
    11461 taatacttac tttatttagt gtaattattg gtacagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggaatgtata cttggctgaa aaggggaact
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgaaaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agccgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagag tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggttca gccaacctag attatataaa ggtagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagaacttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa gccagacact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aagcataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagtgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttgtgg caactttagc ttatttaatt ccgatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggcgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taagaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agcacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag agattattga tgtatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcctaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgtaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt aaagacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 gcttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gtcatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtacttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttata tgaagaacta cttatgtcag
    20641 aagaaggtct gttggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtgccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggtg ttaatagcag
    21121 gagttgtcac agtagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tatagatggg gagcaactgg accaaatgct tttgattgtt
    21481 ctgggttcac ttcttatgtc tataaaaatg gagcaggagt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttcaggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaag aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg ttttttttat aataaattat ttcatattat aatcaaatat caaatataaa
    21961 ttttaactat attattcaat atttgacaga taatatgaat tttagatata cttaataaga
    22021 agtatattgt agaaaaaata aatttaaaaa attaaggggg atgaaatgaa aaaaataagt
    22081 atattagtat tatcattgat aatgacactt actatgtgta gtgtaagttc atttgctgat
    22141 tcatcaaatg ataaagaaat gagagctgct tggatatcta cagtttataa tttagactgg
    22201 cctaagacta agaataatga agccaagcaa aaaaaggaat acacagattt attggacaaa
    22261 ttaaaaagtg taggaataaa tacagcagtg gttcaagtaa gaccaaaatc agatgcttta
    22321 tataaatcaa atataaaccc atggtctgaa tatcttaccg gtacacaagg taaagaccct
    22381 ggatatgacc cattaccatt tttaatagaa gaagcacata aaagaggaat ggaatttcat
    22441 gcatggttta atccatatag aataactatg gcagatgaga gcatagataa attgccagca
    22501 aaccatccag caaagaaaaa tccaagctgg gtagttaaac atggaaataa gtattactac
    22561 gacccaggtt taccagaagt tagaaagtac atagtggata gtatagcaga agtagttcaa
    22621 aattacgata tagatggagt tcattttgat gattacttct atccaggagt aagtttcaat
    22681 gatactgcaa cataccaaaa atatggaaaa ggacaaaata aggatgattg gagaagagaa
    22741 aatgtaaata ctctacttag agatgtaaaa gcgtctataa agagtataaa gcctaatgtt
    22801 gtatttggtg taagcccagc aggtatatgg agaaataaga gtagtgaccc aacaggttca
    22861 gatacatcag gtaatgaaag ttatgtaggt acatatgcag atacaagagc atggataaaa
    22921 caaggattga ttgactatgt agttccacaa ctttactggc caattggtct taaagcagca
    22981 gattattcta aattagtggc atggtgggct aatgaagtaa aaggaacaaa tgtagactta
    23041 tacataggac aaggtatata taagcaaggt caatcgagtt atggtggaca aaatatagca
    23101 aaagaaatag tacaacaagt aacattaaat agaaagtata gtgaaataaa aggtagtatg
    23161 tatttctcag ctaaagatat agcaaatagt acttctatac aaaaggattt aaagagttta
    23221 tattctagta gtgaagaacc agttactcca ccatctaatg ttaaagtgga gaaattaaga
    23281 ggcgacgaaa gatatgacac agcagtagca ataagtaaaa aaggttgggc tacaaattca
    23341 gatacagtag tattagtaaa tgggtactct atagttgatg gtataacatc aacaccactt
    23401 gctacatcaa atgatgctcc tatactattg gtaaataagg ataatatacc tacatccact
    23461 aagaatgagt taaaaagact taatccaagt aaagttattt taattggagg aaataactct
    23521 attggagata aagttgagag tgagataaaa gatacattgt caaatgtatc aataaataga
    23581 gttggtggtt cagatagata tagtacatct ttaatgatag caaaagaatt agtaaaaact
    23641 aatccagtag aaaaactata tataacaagt ggtacaggtg aagcagattc tctttctata
    23701 gcttcaaaag caggagaaga aaagcaacca atagtattag tatcaaaaga taatgtaagt
    23761 gatgaagttt acaattggat atcagattta aaagtaaaag atgcttattt tataggtggt
    23821 aatctaagta tatctgattc agtaataaat aagctggata aggtcattac aaatgatgta
    23881 tctaaaaata gaatagctgg agaaaataga gaagaaacaa atggaaaagt aatacaaaaa
    23941 ttctatccaa atgctgaata ttcatcaatg tttgtaagta aaagtaatca attggtagat
    24001 gcattaacat caggtccttt agcggctaaa ttaaaatcac cagtagttat gcttggaaat
    24061 agtgtaacat ctgctcagaa aactgcttta gagcctaaga aaactactct agtatacgaa
    24121 gctggagatg gtataaatca aaacacatta aacactttct taaacttagt taagtaataa
    24181 aaaagttaat ttaaaataca gagtatttta caaatgtaga ttaaaatgaa aattatcatg
    24241 ataaattaga aaataaaaaa gaactatatg cattttgtaa tcaagtgtac acagcaactt
    24301 gaaaataatt atgcgatata gttctttttt agtaattaat cttatacttt ttaataaact
    24361 ttatgttgaa ttttttattg tttaaactct atgtcattaa atataaaatc gtgtaatatg
    24421 tctaaagtat ctgggtcaaa tctaagaacc catccagctt ttccatatat accacctgta
    24481 ctatgaattt catcatcaat aggaaattca aattgcttaa tatttaagtc acccattttt
    24541 aaaacttgag ctcctaaaga aagtattgca ttaggcttca tgttagtttt aacatatgga
    24601 agaactgcat ctaataaacc tggatattta gtaacaggta aagtttccgt tttcttgatt
    24661 atagcttgta taatctgtct ctgtctgcca tctctagcaa aggctgtatc gttgtgtctg
    24721 atacgtgcaa aagatagtgc ctgatatcca tttagagtct gctttccaga gtttctaatg
    24781 tattgtatgc tacctttatc atcactttta ctccatttat aagtttcagg aataaattta
    24841 tttagttctc gtatttctcc tttttcaaca gtaacctcaa cgccaccaat agcatcaatt
    24901 atatacataa atgattcgaa attaacacaa gcataatttt gaatatcaat attaaaattc
    24961 tcttcaattg tttgtattaa taaatcagct tgtccataag cataagcatg agtaagtttt
    25021 tgtttaccat gaccagggat gtctacatag ctatcacgag ctaatgatgt aagtttgata
    25081 tcattgtgtt tgttatcaat agtaagtatc atcatagcat ctgaccttga agaatcttca
    25141 tttggtctag catctgttcc cataagtagt atgttaatta tcccatcttc atttttatgg
    25201 tcattgttat ccaataaatc actacttatg ctcgagtcat gaatagcaga taatttataa
    25261 taaaaatatc catagacact tatagggaat attactacca agaaagccag tagtataaca
    25321 aatttcttta attttgacaa aagtacccct tctttcttct ttttcctgta tattttttaa
    25381 tagcatacac ttaattatat taaagtattg agatatattc aatacttgtt tcagttacaa
    25441 aaaaaagaca atgatataat ttataagaaa atttatcgac ctatatatat agttaaaagt
    25501 atatataata tattttttaa aagtaattta tattttaaaa taaaaacaca ccccataaat
    25561 acaaagtaat ttgcatttaa gaggtatgta tttatcatca aattattggt ttgttttgga
    25621 atttctaatc ttcaaccata atatctttaa atatgaaatc atgcattgca tctagttcat
    25681 attcctcaaa aggtattaca tagcctgcat tacctatttt tctaccattt tcagtagaaa
    25741 gaggaaactc catagatttc aaatttaaat ttcctatact taaaagttct tttcctaaag
    25801 atattatttc attaggattc atattagttt ttacatacgg aagtattgtg ttaactagat
    25861 taggatattt agtgactggt aaatccttaa caccatttat taaactctgt ataacacttc
    25921 tttgtcttct atctctttca tctgtactat cattttttct tatacgagaa aatgcaagtg
    25981 tctgatatcc atttaatttt tgctcaccag aatttcttat atatttaaga gaacctttat
    26041 tgtcattttt attccattta taagtttcag gaataaattt atttaattct cttatttcac
    26101 tcttatcaac agtaacagta actccaccta aagcatcaac tatatccata aaagagaaaa
    26161 agtcaactac ggcgtagttt tgtatatcaa gttggaagtt ttcttctata gtttctatta
    26221 ataagttagc ttgtccatat acgtaggcat gtgttaattt ttgttctcca tgtcctggta
    26281 tattaacata agtatctcta tttaatgaag ttagcttaag acttttattt ttattatcaa
    26341 tagtaagtat catcatggca tcagaacgag aaacttcttc tcctggtctt gcatctgtac
    26401 ctaacaataa tatgtttgtg atattttctt cgtttttata gtcattacta ctaagcgcct
    26461 ctgtatttat actatcatct tgtatagttt ttaatttagt aaaaacatat ccatatatac
    26521 taacaggaaa tgctataact aaaagggcta gaaatataac gaactttttg agtcctgaca
    26581 ataatatccc ctactttcta aattttttaa ttacacatag ttaattatat taaaaatttg
    26641 ttacataagc aataaaaaac acaataaatt agcataagtt tgtcataata taattatttt
    26701 tatagcattt tatagaagaa agtgtgtagt tattattgat tttattggaa tataattgta
    26761 tgaagactta aatttttata aactaatgtg tatattgaaa aaaatatttt cctgaatgta
    26821 gttatagcat cttatgaaag gtgcaaattg taatttaaaa tttttagtgt ataataagaa
    26881 ttgaaaattc aaaccgataa aaattaataa aaaataatta taaattaata atttggggga
    26941 ggaaatgtat gataaaatta atagcaacag atttagatgg aactttgtta gatgaaaaga
    27001 gcgaaataaa tccagagttt tataaagtat ttaaaaaatt aagagagaga gggataatgt
    27061 tttctgctgc tagtggcagg cagtatcaaa atctgataaa aaaatttgaa gatataaaag
    27121 atgatatgat gtttatatca gaaaatggaa cactagtagt atataaagga aaagaaatat
    27181 tatcaaatcc tttaaataaa gaattagtaa atgaaatcat agagacaaca agaagtataa
    27241 aagggaaaaa aattgtaatg agtgggaaaa aatacgctta tatagaaagc aaagatgaag
    27301 cgtttataca agaggttagt acttattatg caaagtttaa aattgtagaa gatttgacga
    27361 aagtagaggg agacatattg aagatagctg tgtttgactt caaaggtgca gaacataata
    27421 ataatatcta ctttgaaaag ttttctgata gagctcaagt atgtatttct ggagtggagt
    27481 ggcttgattt aactgcaaaa ggtgcaaata aagggagtgc cataaaaaaa gtacaaaaaa
    27541 tgttagatat aaaatatgaa gaaactatgg tatttggtga ccagttaaat gatgtggaaa
    27601 tgatgaaaag tgcctatcat agttatgcca tggaaaatgc aaatgaacat ttgaaacaaa
    27661 ttgctagatt tagagcaaaa agaaatactg aaaatggcgt tgtagataag ataaaagagg
    27721 ttataaaaat tggatagtca tatgtataga tgaaaaagat tattttgaag aaatcaaaaa
    27781 aatatcaatg taattttgat aaataagttt atttatgcga aaattacatt gatatttaat
    27841 aaacatcaat aatagatatt tattttttta tgatattcag tattttgttt tctatttgag
    27901 ttgcattttc agcagaaatt aaataaacat acctacctgc cttctcaatt atagtattat
    27961 ttgctatttc agaattgatt ttttctccat gtccttctcc aaaaggtgct tgtatcatat
    28021 cttttttata attttctaga gttttataga ttttatctac ctcgtcagtt tttataacga
    28081 taatatcttc tagagataat ttttcagaag aattaattac aaaaccttga attatgtctt
    28141 cctctacatc attaaaatag ttaaaatcct ttgcatcaaa gatttttgat tcctttataa
    28201 gtaaatcaga agattttaat gcttttttta tttttgatgt agaaacgttt ttgtaatcac
    28261 cacttgaaga acaactaacc attcctaatg ataagattat cgaaaaaaac aatattaaaa
    28321 tttttttcat gataaacctc caagtttttt aaactctgtc ttaaattagt ttactacata
    28381 attatataaa aataaatgta tttaatgtta cagttttgat taataatgta atattttgat
    28441 aataatacct attgagaggt gattagaaat gagaatacca aaacatgtag gaattatacc
    28501 ggatggaaat agaaggtggg caagtcaaaa tggtatgaca aaagataaag gatatgaaag
    28561 tggaatagac cctggactag agtcttatag attatgtaga gatttaggct gtgaagaggt
    28621 gactttttat ggatttacaa ctgataatac aagacgtcca tcagtgcaga caaaagcttt
    28681 tacaaaagca tgtattgatg ctgcaaatat attaatagat gaaggtgctg ctttattagt
    28741 tgtaggaaat acaaaatcgc caatgtttcc agaagaatta ttaccatata ctaatagaat
    28801 taaagccaaa gaaggtcaaa taaaagcaaa tcttctggta aattatggtt ggtattggga
    28861 cttaaataat atatcaggag tagaaaatgt aaataaaaat aatatacagg attacataca
    28921 gtcatttgat gtatccagac tagacttgat aatcagatgg ggtggaagaa ggagattaag
    28981 tggtttcctt cctgtacaat caatttatgc agatttttat gttatagata attattggcc
    29041 agattttaag agtgacgata taaaggacgc atttagttgg tatgatacac aagatgtcac
    29101 tttaggtgga taattaaaaa tattctgatt taaggttgta gagtgtttta aattattgaa
    29161 aaaaaaactt cttgatggta aaataacact aataatatgt aaaagtgtaa aaatttatac
    29221 caagaagggt gaagaaatgt caaaaggcaa taataacaat aattcaagaa acaaatctaa
    29281 aaagaccagt catctgaata gaaaaaagcg aaaattaaat aagaaaaagt tagcagtgtt
    29341 gatttgcttt acagttttat ttttatttat tgcatttaag gctactcaag gtgtagtagc
    29401 tttggtaaaa agtatggata aaagtaataa aactagtcaa caacaaaatg ttaactcaga
    29461 acaatttgac cttggtaatg aagaggaaaa taaaaagaaa aaatatactg tatttataga
    29521 cccaggtcat ggaggcaatg ataaaggaac tgaaagcaag accagtaata gatatgaaaa
    29581 agacctaaat ctacaaattg caaaaaaatt agcaaataaa ttatctaaac aaaaggatat
    29641 tcaagtagta gtatccagaa ctgatgatac atatatatca ttaaaggata gagctatctt
    29701 agcaaataat agttctgctg atgtgcttgt ttctattcat ttaaatgcag agaaaaatgg
    29761 taatacagca acaggtatag aaacttggta taggaataaa gctacagatg gttcaaaaga
    29821 acttgcacag acagtacaaa gtactatagt atcctatgtt aaagttagag ataggggaat
    29881 agttgaaaat aactttgaag tacttagaga atcaaatatg cctgctatat taatagagtg
    29941 tggtttttta acaacacctt cagaggaaca aaaaataata aatgaaaaat atcaagacca
    30001 acttgcggaa ggaattgttc aaggtgtact ttcataccta gattctaaag ggaataagta
    30061 aattaacttt actttaatgt gaggataaat ttaaaagttt aactgtgtaa attgtattaa
    30121 ctttatttcg taatgttgga aatattgttt gaatttagaa actatgaaat tagaaattat
    30181 tttatctata tctaatttca tagttttttt tgcaaaaaat ttatagttat aaaattttaa
    30241 aaaataaatt tagtataatg taatgtagtt atttatatgt gattttaact tgcattgatt
    30301 tgctatcaat tgtgctttat aattagtata tataaagttt aactgtaaga taatttaaaa
    30361 aataggggaa tttatatgaa taaaaatagc atgacaagaa tttacagttt aaataaaata
    30421 atttttatta taattggaat tttaggaata gttttactca caagacatat tgaggtagat
    30481 attaaagttt taattttaat tttattaacc ttgtttagta taaatgtatt tatttcatat
    30541 cttaaaatta agttgtatga ggataaaatt aatttaggat tgataaataa ttcagataag
    30601 tttataaata ttactaaaga tgagaacttc ctaaaacatg ttcaaaatta tattatcagt
    30661 aatgaaaaag aaaactgtgt aatggcttgt tttgatttat gtagactcaa acttataaat
    30721 gatatatatg gttatgagct aggggatgaa gtgttaaata atattttaag taacttaaaa
    30781 aattattttg gaaaagaagc tatttatgga aagttaaaaa gtgatgtttt tgtattaata
    30841 ataaagcttg aaaataggga acagaagatt ccttatttag taaatcttat taagaataaa
    30901 attgtgatac taagtcagat tgacagtttt aaaatggata taaatattga ggcttctatt
    30961 ggaatttata aatttaacaa taatgaaatt gatataaaaa aagctataga taatgctgat
    31021 atggcaaggc ttaaatcaaa aggactaaag cacatagagt atgtagaatt tgataatgct
    31081 atggaagaag aaatacaatc aattatgaaa atagagagag atttattctt ggctattaaa
    31141 aataaagagt ttgtaattca ctatcaacct aaagttgatt caagtacagg taacattata
    31201 ggttcagagg cattgataag atggttgcac ccatcattag gcatggttgg accaaataag
    31261 tttataccta tagctgaaaa aaatggtctg ataaacaata ttggtagatg gctaattcaa
    31321 gaagttttta ttaccattaa caagtggatt tcagaaggta taaatgtctt accagtctca
    31381 ataaatcttt ctagagtaga actatataaa aatgatttag ttgatttttt taaactgatg
    31441 tttaatacat ataatattcc aaaagaactt atagaattag aaattacaga aacgacagca
    31501 ctaagagatg taaaatttat aagtgaaagg ctttatgaga ttaaatcttt aggtatgaat
    31561 atatcattag atgattttgg agtaggtaat tcaaatttta tcaaccttaa ggggatacca
    31621 ctagatatta taaaaattga taggtcactt atattggata tagttacaaa tacaaaaaca
    31681 aagtttatgg tgaaagcaat tgttaacctt tcacatgatt taaacgtaac tgttatatgt
    31741 gaaggggtag aggatatgca tcaggtcaaa gtcttaagtg aactgggatg taatttcata
    31801 caaggttatg tattttttaa acctttggat gagatgaatt ataaaaaact tcttactgac
    31861 ggaagttata taaatctgaa agatactctt ttagataaat gcatgacagt tgaggaggag
    31921 taaggatgat ttcagaagat gtagattatt caataagaat cataagagct atatttaata
    31981 atggagaaga taaaccaata gatgctaaaa tgatattaaa aagtgaagat ataccaatac
    32041 taacactata cagactgctt agaaagcttt gtgcaaacaa tattcttact tttaacaaag
    32101 aagatggcaa gacatacatg ttaaatgtgt cattggatac aataagtcta agaactattg
    32161 tagaagtaat tgatggaaaa atatatataa ataaatgtac gagatatgta tctaattgca
    32221 gtaattgctt aaaaggttgt aaagtaaatc gtgaattgct agaaatagaa aaagaaattt
    32281 ataaactttt atcgagaaaa tctataagac aaatactaga ataaaaaatt ttcagctatg
    32341 ataataattg gaggttttcg gttatggatg atatatctca agataatttt ctactgtcta
    32401 aagagtatga gaatagctta gatgtagata ctaaaaaagc aagtggaata tattatactc
    32461 ctaaaataat tgtagattat attgtgaaaa aaactttaaa aaatcacgat ataattaaaa
    32521 atccatatcc acgaatactt gatatttcct gtgggtgtgg aaattttctt ttagaagtat
    32581 atgatatttt atatgactta tttgaggaaa atatatatga attgaaaaag aagtatgatg
    32641 aaaattattg gactgttgat aacatacacc gtcatatatt aaactattgt atatatggtg
    32701 ctgatattga tgaaaaagct ataagtatat taaaagatag cttaacaaat aaaaaggtag
    32761 taaatgattt agatgaatct gatataaaga ttaatctatt ttgttgtgat agtcttaaaa
    32821 agaaatggcg atataagttt gattacatag tgggtaatcc accctatatt ggtcataaaa
    32881 aacttgaaaa gaaatataaa aaatttctcc tagaaaaata tagtgaagtt tataaggata
    32941 aagctgactt atatttttgt ttttataaaa agattataga tattttaaag caaggaggga
    33001 ttggcagtgt tataactcca agatattttt tagaaagtct atcaggtaaa gatttaagag
    33061 aatatattaa atcaaatgtc aatgttcaag aaatagtgga ttttttaggt gcaaatatat
    33121 ttaaaaatat aggggtatct agttgtattc taacttttga taagaaaaaa acaaaagaga
    33181 catatataga tgtatttaaa atcaaaaatg aggatatatg tataaataaa tttgaaacat
    33241 tggaagaact tctaaagagt agcaaatttg aacattttaa tataaatcag agattgctat
    33301 ctgacgaatg gatacttgta aataaagatg atgaaacttt ttataataag atacaagaaa
    33361 aatgtaaata tagcttggaa gatatagcaa taagttttca aggaataatt acagggtgtg
    33421 ataaagcgtt tattttaagt aaagatgatg taaagttgaa ccttgtagat gataaatttt
    33481 taaaatgttg gataaaaagt aagaatataa ataaatatat agtagataaa agtgaatata
    33541 ggctaatata ctcaaatgat atagataatg agaatacaaa taaaaggata ctagatgaaa
    33601 ttatagggct atataaaaca aaattagaaa acagaagaga atgtaaaagt ggaataagga
    33661 aatggtatga acttcaatgg ggtagagaaa agcttttctt tgaaagaaaa aagataatgt
    33721 atccttataa atcaaatgaa aatagatttg ctatagatta tgataataat ttttcaagtg
    33781 cagacgtata ttcttttttt ataaaagaag agtatttaga taaattttca tatgaatatc
    33841 tagttggaat tttaaattcc agtgtttatg ataaatactt taaaataact gctaaaaaaa
    33901 tgagtaaaaa tatatatgat tactatccta ataaggttat gaagataagg atttttaggg
    33961 ataataatta tgaagagata gagaatttat caaagcaaat aatatcaatt ttattaaata
    34021 agtctataga taaaggaaaa gtagaaaaat tgcagataaa aatggataac ttgataatgg
    34081 attctttagg tatataagca gttgtaagta ttaaaaaatt ataatctttt aattgtttat
    34141 ttaaatttag aaataaaaag ttagccatat aaaaaatatt aaaaatatat acttttcaat
    34201 atttttttga taaaataaga atgaatttgt gcaattttat ttttctccaa taactaaaaa
    34261 tagttttttg aattaatatt tgcttaagaa taaaaatcag aaacgttttt gatatagtag
    34321 gtgataatat gaaaggatta gttttagaag gtggaggaac aaaaggagcc taccaaatag
    34381 gtgcatataa agctttaaga gagcttggta tagagtttca aggagtaact ggcacatcaa
    34441 ttggtgcttt aaatggagca tatataattc aaaatgatat tgaagtaatg gaagatattt
    34501 ggttaaatta tgactatact cattttatga atattgatag agaaacctat gagaggttta
    34561 aaaatgtaga tttcactact aaaaatctca atgcagtgtt tgagcttata aataaagcta
    34621 gaaaaaatga aggtatagat ataagcccat taagacaatt aatggagaca acattaaatg
    34681 aagacataat aaggaaatca aaaaaagact ttggacttgt tacggtctgg tgggataaaa
    34741 aaattaatcc atatccattg tatatcgaag atatacctaa aggaagattg attgattatt
    34801 taattgctag ttccagtttg cctatttttc aaacgaacat gatggatgat aagttgtatc
    34861 ttgatggaat gttttgtgat aacatgccaa taggactctt ggaagacaaa ggttatgaag
    34921 atgttgttgt aattaggttg attgatgatt ttattgggaa aatgaatcta aatcgacatc
    34981 aaaatataaa tataaaaact ataataccat cagaatatct aggaggttct cttaataaag
    35041 atagagataa tgtagaaaaa aacatccgac taggttactt agatactatg aaagcatatg
    35101 aaagatgcac tggaattaag tattattttg atgtagagta taaatataat gaagaatatt
    35161 gttttgataa atttagaagt atgagcaatg aaacaataca atatatctgt aatttattga
    35221 atataaaaag agatgcttca cttagaactg ttatggaaag tgtaatacca aagcttggtg
    35281 aggtgcttgg attgccaaag gatttttcat ataaagattt gttttactgt atatatgaaa
    35341 agaaacttga agaaaataat ataaacagga taaaattata tgattttaac aaggttatag
    35401 atgttgtaaa tacaaatatt gattctaata gaaatttagc ttctaatttt gaagttagac
    35461 ctaaaattag tattaaaaag aacaaaaaca caaaattatt gacaaattgt attataaaag
    35521 acataaaaaa tcaaagttaa ataaaaaaca aaaaaaaatt tgtaattata ctcgaaaaaa
    35581 gtgtatatta gtagtataat atttttgtag cactagtggg aacaaaaaat gaccatagga
    35641 caaaaaagta ccacttagta ccattaaagt aaaaaacagg aaaacaattt taaaaaatat
    35701 aaaatgtaaa gtttatagga ggatttaaaa aaatggaaaa agtagttagt atcaaaaatg
    35761 caagtggatt acatgctaga ccagcaggaa tgtttgttaa gaaggcagct gaatttaaat
    35821 caacagtaga ggttgtagca aaagacaaaa ctgtaaatgc aaaatcaata atgggaataa
    35881 tgagtttagg tttagctcaa ggagaagaat taaagttagt tgtaaatgga gaagatgaag
    35941 aagtagcttt agctgcaatg gttgaattag ttgaaagtgg atttggagaa tagatataat
    36001 aattttttaa aaatttgata tttagaatct aatattactt aaagtaaagt aacagagaag
    36061 tattaaacat atacagacca acatttgtag atatttaatt tctttgttac tttcatgttt
    36121 atttaatcag aagattaaga ctattgttaa ttgggtataa aaatataaat attttagaaa
    36181 atgaaataag atatttttaa tcat
//
LOCUS       DAMJWS010000035        35161 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMJWS010000035 DAMJWS010000000
VERSION     DAMJWS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.035"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            158..271
                     /locus_tag="PC399_001848"
     CDS             158..271
                     /locus_tag="PC399_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2592708.1"
                     /translation="MIMGRHLWNPSYFVATVLENTEEQIGKYINNQKKQVK"
     gene            273..1391
                     /locus_tag="PC399_001849"
     CDS             273..1391
                     /locus_tag="PC399_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2592709.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNVLKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNAAISKEPSGR
                     YYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNSKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            1542..2759
                     /locus_tag="PC399_001850"
     CDS             1542..2759
                     /locus_tag="PC399_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF2592710.1"
                     /translation="MLIGLIAAFGLVLQKKKASDVIKGSLLAAFGIIIMETGTGMLVS
                     SIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAIHLMIARFTPIKTIF
                     LTGHMLYWFPFIFVASGVEAGLNGGVLITFAAVLSALYWSIMPWLLRKYVFDVIGDDS
                     FTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREISISGGMAVFLMFIVV
                     GIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQIMPAFQGISEKVVPN
                     ALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILIYNYFNVFNLLLIPLVITSFFECG
                     AAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNWILIFGGNDLSLFGI
                     ISRFIGSLFGGIL"
     gene            2762..3502
                     /locus_tag="PC399_001851"
     CDS             2762..3502
                     /locus_tag="PC399_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2592711.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            3825..5135
                     /locus_tag="PC399_001852"
     CDS             3825..5135
                     /locus_tag="PC399_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2592712.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            5233..6150
                     /locus_tag="PC399_001853"
     CDS             5233..6150
                     /locus_tag="PC399_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2592713.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            6166..6975
                     /locus_tag="PC399_001854"
     CDS             6166..6975
                     /locus_tag="PC399_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2592714.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDTMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            7033..8781
                     /locus_tag="PC399_001855"
     CDS             7033..8781
                     /locus_tag="PC399_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF2592715.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVSTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            9043..10065
                     /locus_tag="PC399_001856"
     CDS             9043..10065
                     /locus_tag="PC399_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF2592716.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            10070..12376
                     /locus_tag="PC399_001857"
     CDS             10070..12376
                     /locus_tag="PC399_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2592717.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            12348..13463
                     /locus_tag="PC399_001858"
     CDS             12348..13463
                     /locus_tag="PC399_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592718.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            13481..14899
                     /locus_tag="PC399_001859"
     CDS             13481..14899
                     /locus_tag="PC399_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2592719.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPNIILPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            14914..16122
                     /locus_tag="PC399_001860"
     CDS             14914..16122
                     /locus_tag="PC399_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592720.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(16196..17584)
                     /locus_tag="PC399_001861"
     CDS             complement(16196..17584)
                     /locus_tag="PC399_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2592721.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            17925..19259
                     /locus_tag="PC399_001862"
     CDS             17925..19259
                     /locus_tag="PC399_001862"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF2592722.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(19401..20318)
                     /locus_tag="PC399_001863"
     CDS             complement(19401..20318)
                     /locus_tag="PC399_001863"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF2592723.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            20526..21410
                     /locus_tag="PC399_001864"
     CDS             20526..21410
                     /locus_tag="PC399_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF2592724.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            21623..23464
                     /locus_tag="PC399_001865"
     CDS             21623..23464
                     /locus_tag="PC399_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2592725.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            23769..24476
                     /locus_tag="PC399_001866"
     CDS             23769..24476
                     /locus_tag="PC399_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2592726.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            24470..25780
                     /locus_tag="PC399_001867"
     CDS             24470..25780
                     /locus_tag="PC399_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2592727.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            25897..26580
                     /locus_tag="PC399_001868"
     CDS             25897..26580
                     /locus_tag="PC399_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592728.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            26573..28960
                     /locus_tag="PC399_001869"
     CDS             26573..28960
                     /locus_tag="PC399_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2592729.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAIYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            29240..30382
                     /locus_tag="PC399_001870"
     CDS             29240..30382
                     /locus_tag="PC399_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2592730.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            30401..30925
                     /locus_tag="PC399_001871"
     CDS             30401..30925
                     /locus_tag="PC399_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2592731.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            31025..31843
                     /locus_tag="PC399_001872"
     CDS             31025..31843
                     /locus_tag="PC399_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2592732.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            31921..32232
                     /locus_tag="PC399_001873"
     CDS             31921..32232
                     /locus_tag="PC399_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF2592733.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(32359..34362)
                     /locus_tag="PC399_001874"
     CDS             complement(32359..34362)
                     /locus_tag="PC399_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2592734.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYENMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITYPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDSSI"
     gene            complement(34515..35153)
                     /gene="recX"
                     /locus_tag="PC399_001875"
     CDS             complement(34515..35153)
                     /gene="recX"
                     /locus_tag="PC399_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF2592735.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        13624 a         3811 c         5980 g        11746 t
ORIGIN      
        1 cttacggttc ttatatatgt tatttaaaaa taaatataaa aaaccttcaa aatacttgtt
       61 ttaagtaatt gcatatttta atattatgat aagtgatgaa aaatggaagt tactcatggt
      121 agaggatatg catatagtat gtcaaatata gacatccatt attatgggga gacatctttg
      181 gaatcctagt tatttcgttg ctactgtatt agaaaataca gaggagcaga taggaaaata
      241 tattaataat cagaaaaaac aagtaaagtg aggtgatagt agtggaaaaa gcttataagt
      301 ttagaatgta tccaaataaa aaacagcaag agttaattaa caaaactttt ggatgttgta
      361 gatttgtata taataagtat cttgctaaaa gaatagaagt ttataaaaac gataaagaaa
      421 cttttactta taaacagtgt agttctgatt tgaccaattt aaaaaaagaa ttaaagtggc
      481 ttaaagaacc tgacaaattt tcactacaaa acgtcttaaa agatttagat aatgcatata
      541 aaaaattctt taaagaaaaa gcaggatttc ctaaatttaa atcaaagaaa attaatagat
      601 tttcatataa aactaacttt acaaatggaa acattatgta ttgtggtcaa catataaaac
      661 tacctaaact tggtatggtt aagataaggg ataaacaagt acctcaaggt agaatactta
      721 atgctgctat atctaaagaa ccaagtggta gatattatgt atcattatgc tgtactgatg
      781 tagatattga agcatttgaa aatactaata atcagatagg tttagattta ggaattaaag
      841 aattttgtat ctcaagttgt ggagaattta tagaaaatcc taagtatctt aagaagtcat
      901 taaacaagct tgctaaatta caaagagaat tatcgagaaa aacaatcggt agtttaaata
      961 gaaataaggc aagattaaaa gttgcaagac ttcaagaaca tatagctaat caaagaaatg
     1021 attttctaca aaaactatct actaaattaa taaaagagaa tgatattatt tgcatagaag
     1081 atttacaagt aaagaatatg attagaaata gtaaactatc tcgcttaatt tcagatgtat
     1141 catggtcaga atttattcgt caattagaat acaaggctaa ttggtatggt agacaaattg
     1201 taaaggtagg taaattcttt gctagttcac aaatatgtaa taaatgtggg tataaaaacg
     1261 aggaagttaa ggacttaaat ataagagaat ggatttgtcc tagttgtaat gaaactcacg
     1321 atagagatat aaatgcaagt ataaatatat taaaagaagg attaagacta ataacgattc
     1381 aaaataaata acatatgtaa gaaccgtagg aactacgggg atagcctgtg gagaattagt
     1441 aagacatatt ttagtatgca aaattctatg aagcaggaac cctgtgactt tagtcatggg
     1501 aggttcagtt tatttcaaat aatattttac gtaatccatc tatgttaatt ggattaatag
     1561 cagcatttgg tcttgtacta cagaagaaga aagcatctga tgttataaag gggtcactac
     1621 ttgcagcttt cggaattata attatggaga ctggaacagg tatgttagtt agttcaatag
     1681 caccaattaa tggagctttc caaagtataa gtggtggtgc agtaacaaaa ggtttaagtg
     1741 atgttacttt tactgcaagt tatggtggaa ttataggttt agcaatgttt gcgggtttag
     1801 ctattcacct aatgatagca agatttactc ctattaagac aatcttctta acaggtcaca
     1861 tgctttattg gttcccattc atatttgtag cttcaggagt tgaagctgga ttaaatggtg
     1921 gagtattaat aacttttgca gcagtattat cagcactcta ttggtcaata atgccttggt
     1981 tattaagaaa atatgtgttt gatgttatag gagatgattc ttttacatta ggtcatccat
     2041 ctggttgttt agcacttata tcaggatttg ttgctaaatt agtaggaaat aaagaaaaat
     2101 ctacagaaga tataaacata cctcaaagtt tatcattttt tagagaaata tctataagtg
     2161 gtggaatggc agtgttttta atgtttatag ttgttgggat agtaatacca ggtgcgttta
     2221 aagatagtaa tgagtttatt ggaacgtatg caataaagca aggtctttct tttggagcag
     2281 gtcttataat aatgcttcat ggggtaagaa tgctaataaa tcaaataatg ccagcatttc
     2341 aaggtatatc tgaaaaagtt gtgccaaatg cactacctgc ttttgactgt ccaatattat
     2401 ttaattataa accaaatgct gtattaatag gattcttagt tgctatgcta acatctacag
     2461 ctatgatttt aatatataac tactttaatg tatttaattt gctacttata ccacttgtta
     2521 taacttcatt ctttgagtgt ggagcagcag cagtaatagg agaatcacaa ggtggtatac
     2581 gtggagctat aataggtaca gctatagctg caatatttat ggtagtttta gtaggaatat
     2641 ctgctgtaat ttactcagga actattcaaa attggatact aatatttgga ggtaatgacc
     2701 tttcattatt tggtataatc agtaggttta taggttcact atttggagga atattataga
     2761 tatgaaattt aaatacatag atgagataag aagattaatt gatataatag aaaacgaaga
     2821 aaaagtcaat atggaaaaag cagtacaaat ctttgttgaa gcaatagaaa gtaaaaatgc
     2881 tatattttca tttggagctt cacatgcagg aatattaaca gaagaattat tctatagagc
     2941 aggtggctgt gttcttataa atccagtatt tgcaaaatct ctaatgttag atacagcgcc
     3001 tataactcac acaagtaaga tggaaagatt agaaggatat ggtacagctc tagctgataa
     3061 aactccaatt aaaaaaggag atgtagttat agttcattca gtatctggta gaaatccagt
     3121 aagtatagag tttgctatga aagctaagga aaataaggca atagtaatat gtataactaa
     3181 tttaagttac tcaaaaagtg tatcttcaag acattcttca ggaaaaaaac tttatgaagt
     3241 atgcgattta gtgatagata atcatggtca aaagggagat gcctgtgtag agatagatgg
     3301 attaaaccaa aaagttagtc caacttctac tgtaatagca actactgtga tgaactctat
     3361 tatagctcaa gctactcagg aattagttaa taaagggcta aagaatcctc cgattttcta
     3421 tagtgccaat attgatggtg gagatgagtt aaataagaag atttttgatg aatataaaag
     3481 tgtaattcat tatgaatatt aaggttaaaa aaatatgtta atgaactttg aagtttatca
     3541 acaaagaata ttaataatat ataataaatg ctatcattta aaagctgtag gtaaaaatct
     3601 acagcttttt attattgtag attaaaaaga tgcgtaatta aattttaaat tggagaaaat
     3661 aaattggaag gtatcaatta taaatattag aaacgttgtc atagaaaaaa actatattaa
     3721 aaaaaagtac atagaaaaaa ctgattagac aataaataca tgctttgata atattttaaa
     3781 tggaatgaaa atgtgtagaa ttataatgaa aaaagaaaat aaaaatgaaa aaagaaaata
     3841 aaatggaggg aataaaagtg ggtatgttaa aaaaattatg ttcactagct atggtagcaa
     3901 ttatgactac aacacttata acagggtgtt caagtggaaa tggaaaagat tcaaaaaagg
     3961 atggcgaaaa agaaaaacta gttctttgga tgccaccagt agaaaataat atgaaggaag
     4021 tttgggaccc tattttagat aaatttgagg aaaaaaataa ctgtgaagta gatttacaga
     4081 taattccttg ggataattat tctgaaaaat ttgcaacagc tataagtgct ggcgaaggtc
     4141 cagatgtagg atatatgtat gcagaaatgt atccacagtt tatagaaagt ggagcagtag
     4201 aagatttgac taactatgca actaaagagg ataaagagca atctatttat ataaaaacat
     4261 ctgaaatgat gggtggaatg tatggtatgc cattccaagc agcaaatcca ggggtattat
     4321 actataacaa ggatatttta gagaaactag gagaaaaacc tcctaaaaca tgggaagatt
     4381 ttaaaagaat ttgtaaaaaa gcaacgcaag atactgatgg tgatggaaag atagaccaat
     4441 ggggattagc tcagggatgg ggagctaaaa cttttggtaa cttaaactgg aactggtatc
     4501 cttatttatg gcaagctggt ggagatatat ttaatgatga tttaaaatcg gttaaattta
     4561 atgataagag tggtttagaa gcagctaatt tcttaaaaga attacaagca tatgtgcctg
     4621 aagattcttt atctaaagat agtaatgaaa tgatagaaag cgtatttgga ccagggaaag
     4681 cagcatttac aataatgtta tcttctgctg caacaagtgt atttgatgaa tcattcccgg
     4741 atttaaattg gggatttgta actggactag aagataagaa agcagcaact tttggagcag
     4801 tagaccattt atctttaatg tcatctgcta aaaataaaga acttgcatac aagttaatac
     4861 aacatatgtt aagtgttgaa tcaatgacag aatttcataa agcaatacca agagcaccaa
     4921 taacaaaagg tgaaccatat caaggtgatg aaagattcaa agaaatggtg gaaaatgaca
     4981 aaaatgtata tagaccatta gtagtaggac ctcatggagt tgaaatatat gaatatttat
     5041 ggaaagaact tcaaactatg atttcaggag ataaaactcc aaaacaagcg ttagatgatg
     5101 ctgcaaaata ttcaaatgat ttattagcac aataaaagtt taaagctgta atatgttgta
     5161 aaggacttta cagattaaaa ttagcattgt atttataaat acaatgctaa tatttatatt
     5221 ggagaggata aaatggaaaa cttagttaga gagtttgaag aaaattcaaa aagaaaaaaa
     5281 acaaaattaa atattaaacc atatatgtat atattgccat tgggaataat tttagtggcg
     5341 ttttatgtga taccaataat aatgtcaata tactttagtt ttacaaagta taatattatt
     5401 agtccagcta cttttatagg tttagaaaat tataaaaaat tatttacaga tgaaacatta
     5461 aaagtatcta ttataaatac tattaaattt actgttgttg tagtaccttg ccaaactata
     5521 ttatcattga ttttggctgt ttggattaca ggaaaaggaa atagtaaaat agcttcattt
     5581 gctaaaggtg ctatatttat accagtatta tcatctatgg ttttgatagg tatggtatgg
     5641 agggctttat tgaatggaga aggttctatt atatatcagg tattaggtgc ctttggaata
     5701 gaatcatcta aacttttagg agatagtaag acagcattac ctacacttat gtttatatct
     5761 atgtggaaaa atatagggta ttttatggta atttatattt cagctataat gaatttacca
     5821 aaacactgct atgaagtagc gaaggttgat ggggctacaa aacttcaaga atttataaaa
     5881 ataactgtac ctttattgaa accaactaca ataatggttg tatttttagg gtcgatatgg
     5941 tcattacaag tatttgactt aatttatact gtgacaggtg gaggtccagg tatatctact
     6001 atgagtatag ttatgcatgc atttaactta aactttaaaa actttaattc tggatatgca
     6061 atgactgtag caaacgtatt atttttgtta atagcagtag tttcgatttt acaaaataag
     6121 cttgtaagac gagacaattc agatttttag aaaggtaggt ataatatgaa aacagcaaga
     6181 agattattaa gtgatggttt gcttttattt atagcgtgtg caagtttggt accttttata
     6241 tacatgttga taatatcatt gaagataaca tataactcat atagtttgga tatatctttt
     6301 tcaacagtaa cccttcaaaa ctatatagat atttttacta aaaaaggatt tgcacaatat
     6361 ttttttaata cagcaatagt atctttttca ggtgtattat taaatttagt atttagtaca
     6421 ttagcaggat attcttttgc gaagatggat tttaaaggta gtgataagtt atttttattt
     6481 atgataatga ctttaattat accttcccaa gttacaatga taccactata tataataatg
     6541 aaacacttag gatggataaa tagttatctt gcacttatta tgccaatacc aactgcattt
     6601 ggcgtattta taatgagaca agctatttta ggagtaccaa aagagttatt agaatcagca
     6661 aagatagatg gttgttcgga ttttagaatt ttaatacaaa tagtgttgcc tttaattaaa
     6721 ccagcattaa ttacactagc aatatttact tttatgggag catggaacga gtttatgtgg
     6781 cctcttatag ctacaactaa agatacaatg agaacattaa cagttggatt atctactctt
     6841 aagacattcc agataactaa ttatggacag atgatggcag gggctacaat aacattttta
     6901 cctccattta ttttctattt gattttacag agtaaatttg ttgaaggtgt gtctttgtct
     6961 ggtataaaag gttaaatagt ctaatttaat aatatataaa gaaacttata taaatatgga
     7021 ggaatttaaa ctatggaaat ttcatatgta ctgaaagatt gcacattcaa acacaataaa
     7081 tactcagttg tagaagctga aacttggaaa gataaggatt tggatattgt agtagctaaa
     7141 ggtgaaaaat ttgcatttca aattatgtta aaagcaacag aagaatttaa ttgtacaatt
     7201 gataaaagta gtgctatatc atggaaaggc ctgggaaata gagtaaggtt agcattaaat
     7261 atgcctaata gtttagaaaa taattttagt ataaatatat taggatatgt tcaagatgat
     7321 acaaaggcat ttgtgaatga tgctatttta agggataaag atgtgttagt agagcaatat
     7381 ttgcctcaaa cattttggat tgaaggtcaa gtaccagaag attttgaaga aaataacctt
     7441 gatataggta ttgatatatt taaaagcttt ggatatgaag acgaagaaaa agtttgtaca
     7501 attgatgttc ctgtaaaagt aagaaatgtt gtgctaaagc ctttagatga aagcaagttc
     7561 tttttagatt tatggcaaca tccaagctgt ttagctagaa tgtataaagt tgagttatgg
     7621 tctgatatac attttgaaat agtcgataac tatttaaaag aattagcatc tttaggagaa
     7681 aaagttgcaa cagttatagt ttcagattat ccatgggcag ggcaaagttg ttataaagtt
     7741 tataaaaatc catcaaactt atatgaatat aacatggtaa gtgtatcaaa aggattggat
     7801 ggaaagatta aatgtaactt tgagagtatg gataggtata taagcatagc tgataagtat
     7861 aaaatggcta aggagataga tttatttggt ctattaggta attggtgtgc tggtgagttt
     7921 ggaaatcctg tagaaggtta taaggaccct ataagagtaa gatattttga tgaaaaggat
     7981 aaagtattta aatttataaa caatacaaat gatttaaaag aatatatagg attagtatta
     8041 aatcatttaa tagagtgtgg attatgggac agagtaagga ttatagctga tgaaccaaat
     8101 aatccagaag tagtcaaaga gtgtatagaa tttataaatt ctactgtaag cactcatcaa
     8161 gttaaatata aatcagcaac tcacgaccaa aactttttag acagagctaa agatgaaatt
     8221 gatgatatgt caataaattt aaaacttact atacaaaatt ataaagatat agaaagtctt
     8281 aagaaaaaaa tcaatgacaa gggtgggata ttgacttggt ttgtatgctg ttttccagag
     8341 aaaccaaata gttttttaag ttctcctttt gtagaaaata gaataatagg atggtatact
     8401 tattactttg gtcttgatgg atttttaaga tgggactata atttatggac agaagaccct
     8461 tggaaagatt caagttacaa attccctatt tggaaagctg gagatatgtt ctttgtttat
     8521 cctggaaaag atttaaagcc agttagaagt gtcagaatgg agaatttaag atttggaata
     8581 caggactttg agttatttac aatgttagaa aaagaaaagg gaagagagta tatagaagtt
     8641 gagcttatgc aagaattatt aaataaaaaa gaaaatgctg aaattaaagg ttttggtgac
     8701 atagaactag gatattcact tgataattgg ggatatgata aagttaaaaa atatgtatta
     8761 gagttgttag acagagtata aaaaatataa gagtgtctca aaatgaactt tttagttcat
     8821 gaggcactct ttttgtatga aatcaatata gtttcattgt ttcaacaatg aaaaatagga
     8881 ttatttctat tctaagacag ccctttagtt ttttagttgt attcaatgtt ttaaaactaa
     8941 ctataaacgc tataaagttt ataaatgaca ataattatat ataacatgat gtactttaat
     9001 ttgctataat agggattgaa taatattata tggaagataa cattgtttgg ggggattaat
     9061 ataaaaagaa taggtattat tataatgttt tttttaagta tatcttttat tggattttat
     9121 cttgtaataa gagataacag tgatacagtt aagatgcaac gagggataaa tataggtaat
     9181 gcactagaat cacctaagga ttttccgtgg gatgtaaaaa tgtcaaataa gttttttgat
     9241 gatataaaag atgcaggttt tgacacagta cgtataccag taaggttttc tgattatacg
     9301 tcagattctg ataattttaa aattgatgaa gaatttttta aaaagattga taaatatgta
     9361 gattatgcat tggataaaga cttaatagtt gtgttggatt tacaccattt tgaagaaatt
     9421 atgaaagaac caagagttca taaagaaaag tttttaaaaa tatggcaaca gatagctaat
     9481 aggtatcaaa aatatgataa aaaattggta tttgaattat taaatgaacc taaagaaaat
     9541 ttatattcac agttactaaa cgaatacatt gaagaagcaa taaagataat tagaaaaact
     9601 aaccctaaaa gaactattat agtaggacca tataatttct atcaaattga ttatttgaat
     9661 gaattaaata ttcctaaaga ttctaatata gtagtttcct ttcactatta cgagccaaat
     9721 gattttgcat ttcaaggaaa tatatatcat aagggatttg agcacttaag caatattact
     9781 tgggaaggaa caaacgaaca aatggattat ttaaaaaaaa ggtttgatac tgtggaaaat
     9841 tgggcaaata aaaataaggt aaaaatcttc ttgggtgaat ttggaataac aaaagaggca
     9901 ccagaagctt caagaagagc atggataaaa gctgtaagag aagaagctga aaaacgaaac
     9961 ttttcatggg cttattggga gcttgcatct ggatttggta tatacaatca aattgaaggg
    10021 acttgggata gagatatatt aagtgcatta atagaaaaga ggtgatttta tgaagaaaaa
    10081 ttcaaaaaag atacttataa ctttaactat tattactaat gtcatatata tattgtggag
    10141 aattttttac acagtaccta aagaagaagg gaagtttgcc ttaatatgtg ccataatact
    10201 tctttttgta gagataatgg gcatgatgga aatgtttgtg cattattatg gtatgtcaaa
    10261 tattgagtac cctgaaaaac caatcataag tgaagaactt tatccacatg tagatgtatt
    10321 cattgcaaca tataatgaat ctgttgattt ggtgagaaaa actgtaaatg ggtgtattca
    10381 tatgcagtat ccagataaga aaaaggtaca tatatacata tgtgatgatg gaaatcgtga
    10441 agaaatgtgt attctagcag aaaaaatggg tgtaaattac ataactagaa ctgaaagaga
    10501 aggagcaaaa gctggaaatt taaataatgc aatgcaacat acaaattccc ctttgattgc
    10561 tacatttgat gcagatatga taccaatgca tgacttttta attgctacag taccatactt
    10621 tttgaaaaat gagcaagcta aaaaagatgg agaaaaagag gagtatgaaa aggttggttt
    10681 tgtgcaaact ccacagagtt tttataatcc agacttgttt caatttaatc tgcactctga
    10741 aggaaggatt ccaaatgagc aagattattt ttacagagac attcaattgg ctagaaatag
    10801 gacaaattct gtaatttatg gtggtagtaa tacagttatt tcaagggaag cacttgaaga
    10861 agttgatgga ttttatactt attctattac agaagatttt gcaacaggta tcttaattca
    10921 aagtaagggg tatagatgtt atgctattcc agaagtacat gcatcaggtt tatctcctac
    10981 agatttaaaa agtcttatta agcaacgtga aagatgggca cgtggatgta tacaaactgg
    11041 tagacgtcta aatattttgt ttaggagagg tttaggtttt tggcagaaaa taagttatat
    11101 ttcttcaatt acttattggt atgcaagtat aaaacgcttt gtatatataa tggcacctat
    11161 tttattttct gtgtttaatg tgattgtagt aaaatgtacg ttacttcaag tattggtatt
    11221 ttggttgcca atgtatattt taagtagttt atctcttaaa atattttctc aaaatataag
    11281 aaatactaga tggaccaata tatatgaaac tataatgttt cagtctttaa tgccagcagt
    11341 aatacttgaa acgtttgcaa tatctaaaaa taaattttca gtaacaaata agagtaaatt
    11401 agaagaaaat agaatgtata agtttttaca gggaatacct tattttattt acatggtctt
    11461 atctatcata ggaattttaa aaatgtttgt agcaatattt aaaatgagtt caatgacata
    11521 ttcagttgta ttattttggt tgattggaaa cctttttaac cttgttatgg ctacattatt
    11581 tatatcaggg agacagcagt taagaaagtc tgagcgttat attgctgaaa ttgattttaa
    11641 gttaaagcaa aattcttatg ttctttcttc aaaaacaatt gatatatcag aaaatgggtt
    11701 tgcatttttg ctagaaaatc cagaatatat ttccccagaa gaagagtttg aggtagagtt
    11761 tagagaaaaa tctggaaatg aaatgtatat agctaatatg aaagcaaaga ttgttaatgt
    11821 tgtggaagta aattcaaagt ggaaatatgc agcttatatt acacatattg aagatagtga
    11881 aatagataat tggatgtgta ttgttcacga tagaatacct actttaccaa tgacaatatc
    11941 taatcaacta ggtttctttg atgatttgca aattaatgta aaaaaacgta ttgaaaaaac
    12001 tagaacatta tcacgaagaa gtccaagaat caatatgaat tttcagatgg atataaaaaa
    12061 tacaggtaaa ttaagaatag tgaattttaa ttatcaatat gtattattaa attttgagaa
    12121 taaaagtatt tatcctaaag aaatagcatt ggaaattaat gaaaatattg tgttagaatg
    12181 tgatttgtgt gagggtaaaa ttgatgaaag aggtatctta tataaagtaa ataacattga
    12241 cagcataatg caaaatttct ttttaagaga tgaaatgatg gattggatat tacaaaataa
    12301 gaaaattctt gattcaaagc ctagtgaaaa gaaggaaaaa agtatagatg agtttgaacc
    12361 catggaatat atttaggaga gatgaaatga agactttaaa agtgtttttt tgttctacaa
    12421 aatatcttat tttggtgttt atttttttta taaatactac ttttataagt tcagcaacaa
    12481 gtgataaaat tttagattta tcctttaaaa agatagagac tgacttaagt tctaagatta
    12541 catatgagga tacaggtgta aaaatagaaa cggatagttc taaaagcgat aaggaacgtt
    12601 atttatatat ttaccaaaat ataaaagaaa attggagcat gtataataat ttttatattg
    12661 aaattcaaaa taaaaataaa agttctcaga gaataaattt aagcatacaa agtaaaaata
    12721 tgcttgaatt tagattaaaa gaaggtagtg aagtattttt agaaggtaaa aatataatat
    12781 attcagataa aataaaagaa ggatgcatag aagtaccagg tgaatttgaa ggcaaaattt
    12841 atgtgaactt taatagcctt ataaatgagg aaagtaatgt tgtgctggac tcaaatatgc
    12901 tttcaaacat tgttagttgg ggaattacgt ttattccatc tgatgaagaa cataatattg
    12961 ttattataaa aaagatatct ttattgtctg aagagaaatt aagattttta aataatatta
    13021 aaataattgg cgatgaagaa gtacaaatac cagtattggg tcaaagtata agtcagtatg
    13081 aggtattagg tcttaaaagt gattctaaaa ttaagtactc tcttatggga aaacaagata
    13141 atgtgagtat atctcaaaac ggtaaattaa cattaagtaa taaagccaaa cctggacaaa
    13201 ttattttaca agttaatgtt gatgataagt ttaaaattgg aaagaaaata acattaactg
    13261 aatcttggag tattaataaa aaagataagg atggtgttcc atatacatta gtatctcctg
    13321 agcaatcacc aacagtacaa gatatgaaaa aaataaattt tatgaataat atcataacgt
    13381 ttgttcgaat tttatttgta tctctagtaa taatatgctt tggaatatat ttatattgga
    13441 aaaaatgttc aaaaactaag taaagagagg aggttagatt gtgttatttt caagtttaat
    13501 ttttttattt tattttttac ctataacttt ggttttatat tatgtattta ggtttaaccg
    13561 aacgatacag aacatgatat tgcttgctgt tagtttattt ttttatgctt ggggagagcc
    13621 aaaatttgtt gttatcatga tagtttccat aataatgaac tatatatttg gtttactggt
    13681 agatagatat agggaaagta aaataaaagt aaaagttttt ttagttttga tgtgtgcata
    13741 caatataggt gttttattta tatttaaata tctagctttt gcgctaagaa atattagtga
    13801 acttattagt actgaattga ctatacctaa tattatttta cctataggaa tttcattttt
    13861 tacatttcaa ggaatgtctt atgttataga tgtatatcgt agacatggag aagtccaaaa
    13921 gaatcctttt tatgtgggac tgtatatagc atttttccca caattaatag caggtcctat
    13981 agttagatat gagagtgtgg cagaacaaat tctcaataga aaagaaactt ggaataaatt
    14041 ttctattggt acttgcaggt tcataacagg tctaggtaaa aaagtattaa tttcaaataa
    14101 catggctata gtagcagact atatttacac tatgaatagt caaggagaaa tagctgcatc
    14161 acttgcatgg cttggttcaa tagcctatac tctgcaaata ttctttgatt tttctgctta
    14221 ttcagatatg gctataggct taggtcttat gtttggtttt aaatttgaag agaactttaa
    14281 ttatccatat atatcaaaat caattagtga gttttggaga agatggcaca tttctttggg
    14341 aatgtggttt aagagctata tatactttcc gcttggaggt tctagagtag caaataaaga
    14401 tattatgatt agaaatatgt ttatagtgtg gttattcact ggaatatggc atggagcaga
    14461 atggacattt gtaatttggg ggattttaaa ctttgttttc ctaattatag agcgatttat
    14521 aatgtttgaa aagattgaaa atcacaattt tataaaacat atttatgctt tattagtagt
    14581 taattttgga tgggtattgt ttaggtcacc taatttaaaa gaagcatata attattttaa
    14641 agctatgttt ggaggcaatg gagctatatg gagtgattat acatatatgt ttttaaaaga
    14701 atactgggtt ttctttatat ttgcatttat ttttagtgta cctattgcaa agaaaatcaa
    14761 taagtttgtt gttgaacaag ctaaatatag tatgatattt aacgtatttt atccaatctc
    14821 agctatttta ttgtttttta tttctgtgac atatttagta actggaagct ataatccatt
    14881 tatttatttt aatttttagt tgggaggaaa agtatgaata gattttttaa agtaagatta
    14941 ataactgcaa ttatgttctt aattatttta ttcgctttct ctatctttaa tgtttgttat
    15001 tctggaaaag atacgataca ggaaataaaa aattcaatag ctgaaaaaag tagtttaaaa
    15061 gatacaatat catctattga taatacagtg aatgacaaag taatatacaa aaatacgttc
    15121 attgaaactt atggctattt acaaaaatta atgggtaaaa atgagttttc aaaatttagt
    15181 gttgtaaaag atacttctgg aaagatgcat tatacatatt ttgctaaagc tccaaatcca
    15241 gttggtgtga tagcagatag ggtaagaaaa ttttctgatg aaatggaaaa gcaaaacact
    15301 aaattggttt acctaatgac tccagataag tatataaaag gagtgacaaa atttccaaaa
    15361 ggaatacctt attcttatag taatgagaca gcagataatt ttttaaatca actaaaaaag
    15421 tataatgtgg actatattga ttttagagaa aatattttaa agagtggtat tccaaaagat
    15481 gatttatttt ttaaaacaga ccatcactgg aaagtagaaa cttctttttg ggcatttgga
    15541 gaattagttg agcaacttaa taaaaaatac aatatgaatt tagatgaaaa tcattattat
    15601 agagataagg acaattataa ttcaattgta tatcctaagt catttcttgg ttctatggga
    15661 agaaagactg gtatcttata tggtggtata gatgatttta cattaattta tcctaaattt
    15721 aagactaatt atacttatta tacagattct aaatctcaaa aatttgaatt aaaaggaagg
    15781 tttgaagaga gtttaatatt atcatatcct tttaatgcag accttgattt aatggatggt
    15841 caatcagata agtattttac atatttattg gggaacagac cattagttaa aataaaaaat
    15901 atagaaaatc cagatggatt aaaagtgctg tttgttaaag attctctaat tgtaccaaca
    15961 gcagcatttt tttcctctgt ttgttcatct atagatatga tagaccctag atattatgat
    16021 ggagatatac tagaatatgc aaaatcccat gactatgact ttgtttttat atctgtatat
    16081 ccccaaaatc ttacaaaaga attttttccg ttttgtgagt aaatttctaa aaaagttttc
    16141 tgataaaata aatggctaac tggtaaaaag ttagccattt attttgagtg ttattctaag
    16201 aaatattttt tttactaaat acttctttat ctatttttcc aagtgatttt cctacagtaa
    16261 ttgatgatac catagcacca ttaacattta tcatagtacg tcccatatct agtatagggt
    16321 ctattgcaag tataccacca actaatggaa aataagaacc aagaccaact ccagagataa
    16381 caactgaaac ggccattgta gcagttcctg gaagaccagc tattccaaac gaactaattg
    16441 ttataacaat taacaacata acataaaagc tcatatccat gtttactcca gccatattgg
    16501 caagtgttac agccattaaa gcaggatata tacctgcaca accattcata cccatatttg
    16561 cacctaaact tgctgtaaag cttgatacac cctcatcaac tccgtgattt tgtactagac
    16621 tttctatcgt tacaggcaat gttcctaaac tagaacgtga agtaaatgcc agaagtaaag
    16681 cttcagttga attttttata taagtaatgg gatttagacc atttaaagtt ataaaaatta
    16741 aatgtataat aaataatatt gcaactgata tataaagagc taatataaac tgtaaaacag
    16801 atactattga actaatacct tgtgaagtta ttgtatttgc aagcagagca accacagcat
    16861 aaggcataaa tttaataaca gtcatagaca cacttacaat tattttgtaa gatgcttcta
    16921 taaaatctat aaatggtttt attgtctcaa aatgtttttt acttaatctc tttattgagg
    16981 tagctataaa tgtagcaaat attattgtag ctacaatatt tccatttgcc atagccatta
    17041 caggattaga tggtagtaat cctcttaaag tatccacaag agagtttatt tctttaagtt
    17101 cttgattttc aactaaagta ggcatattag ctcctacacc taacttcatt atatttgcaa
    17161 cgataattcc aacaattgca gctatagcag tagtagcaag aagcatacct aaagatttaa
    17221 atgtcagagt tccaagattg ttatctttca tatttataat tacttttata atggataaga
    17281 aaactaaagg tactactaac atttttaata agtccataaa tccagaacca aataggctat
    17341 accacttact tacttcttgt agccaagtga cttgagttgt atcattagga aaaccagcaa
    17401 aagcttgtat tatgattccc aatatgagtc ctagtactgt agcatatatt gttctcaatg
    17461 aaaattttat ttttttcttt tcaaattgtc taacaataaa gaacgtacct agaagtaata
    17521 ttatgaaaaa aattgtttta aaatctgtaa tcataagaaa ttgtgataaa aatgaacttt
    17581 ccaatatttt tctcctttta tttaaaaata aatttttcta agtttaaatg atatcaatat
    17641 attaaaaaaa tgtcaatatt atatcacaat ttacttaaat agagtaaaac aagttgattt
    17701 tgaaaaatgt gattaagtac agaaaaatag ggtataataa atgtttaaag aatttgaatt
    17761 gtggtaagaa aataaaaaat agcttttata agatttgatt tcaaattaaa atatgttaaa
    17821 taaataacta aaaattatta taaaaaatgt tttcatagaa tatatatcaa atgaaaagct
    17881 taaaataagt gaagaaatta actagacatc aaaggagaga taaaatgaga gtaataataa
    17941 ttggtggtgt tgcagctggt atgagtgctg cagcaaaatt aaaaagaata aaacctgagt
    18001 atgaagttgt tgtttatgaa aaaactgaaa tagtatcttt tggtgcttgt ggattaccat
    18061 actttgttgg tggatttttt aatgatgctg atgaattatt agcaagaaca ccagagaaac
    18121 ttagagaagc tggaattgat ttaaatatat ttagagaagt cgttgaggtt gatagtgaat
    18181 ctaagaaaat aaaagtaaaa aacataaaaa cagatgaaat atatgaagat tactatgata
    18241 aattgatgat agcaactgga gcaagaagta ttatgcctcc tataaaaaac ataaaattga
    18301 agaatgtttc tacattaaaa agtttatatg atggagaata tctaaaaaaa cttttaagta
    18361 atgaagataa taaaagagtg acaataatag gagctggttt tatagggtta gaagcagttg
    18421 aggcatgtaa gaaattaggt aaagatgtag atgtaattca gttagaagat agaatattgc
    18481 cacaagtatt tgataaggaa atgacggatg tattagaaga agaaataaat agacataatg
    18541 ttaatttgca cttagatgaa atggttgtag aactttgtgg agaagataaa gtagaaaaag
    18601 ttataacaaa caaaggggaa atagatacag atgtagtaat aattgctact ggcgtgagac
    18661 ctaataccga gtttttgtca aatactaata taaaaatgct aaaaaatgga gctatagtag
    18721 ttgatgagta tggaagaact tctgtagaag atatatactc tgctggagat tgtgcaacta
    18781 taaagaatat agtaagtaat gaaaatgtat atgttccttt agctactgga gctaataaat
    18841 tgggtaggat agtaggagaa aacctagcag gaagagaagt atcttatcaa ggttcactat
    18901 cttcaagctg tataaaaatt atggatatgg aggcagcatc aactggaatt acagaaaagc
    18961 aagctaaaga tttaggcata aatgtcaaat ctaaatttat ttctgattat aatcaaactc
    19021 attattatcc aggaagaaat aaaatatatg taaagcttat ttatgatgca gatacaaaag
    19081 ttattttagg cggacaagtg gctgggttta aggatgctgt ccaaagagca aatgtattgg
    19141 cagctgctat ctttggaaaa atgaccacaa atcaacttgg aatgttagat ttatgttatg
    19201 ctccaccatt tgcaagaaca tgggatgttt taaatgtagc tggaaatgta tctaaataat
    19261 atttagatta tagctaatat agttgacaag caattaaggg aatgaattta cctaaactat
    19321 ttttatcatg gtaaaattca ttcctaaatt tttattccaa atttttattc caaattttta
    19381 gaaataagga ttccattaaa ttattcatca aaatttaaac gcaagcttaa tctatcttct
    19441 ccaaggatag tattttcaaa aacattagta gcattttcta aatacatact aggtatgtct
    19501 aaagaaggac taaaatgagt cagtaaaagt tgtttgacat ttccaagttt tgcaagatta
    19561 gcagcttctc taaaagtcat atgtttgttt ctaacagctt ttgatatatc taaatcatca
    19621 ccatacatgg cttcacatat aaataaatca ctatctttta tgaaatctgg tattgattct
    19681 ataggtcttg tatcggttgt aagacttatc ttaactccct ttctcaattc tcctaagacc
    19741 atatctggag tgtattgttt tgaatctaaa actatatttt gaccttcttg gagtttgttc
    19801 cataatattt taggaacttc gtttttagtt gccttatcta tatcaaattt agggtttctt
    19861 ctaaaattaa aactgtatcc taaacactct atagagtgtt ctaaactgat tgttgaaatt
    19921 tctaaatttc ttaaaacttt attatctaaa gaaaaattgc cttgagggtt ttctataatt
    19981 tttaaagtgt atggtacata ttctactaaa tttctcatag aacgtataca atcgactatt
    20041 ccaactggac caactatagt taaatcactt gtacgaccac tatttccaat tgttgataaa
    20101 agtcctaaaa gtccaaatat atggtcacca tgtaaatggg ttatacaaat caagtctata
    20161 tctttaaagc cacatttttt tagcttcata gatacctgag taccttcacc acagtcaatt
    20221 agtatttttc tacctttata gtttataaat acagaggata gaaatctgtt tggcataggt
    20281 acatttccac cacatccaag taaagttaaa tctatcattt tttccccttt ctaaaattaa
    20341 cacaaatctg ttgtttgttt ttattatgat atcctattat aattatttta acattaaata
    20401 taacaataaa tgtgtatttg agtcaagaaa tattgtcaaa taaagttttt atatagaaat
    20461 gtataaaaat ttggaaaaca aaaagtgaaa attatattta ggagcattta aaggaggaaa
    20521 atttaatgaa agtattaaag aaaattacta ttttgtcatt aattacatta accttagttg
    20581 gttgtagtaa cggagaagat aaaaaagtgg ttgaaggttc taagaaaatt caaaattcta
    20641 aaaaagatga acagcctata atagtacata aagatgcaaa tatattgaaa gataggtatg
    20701 agccaccaaa aggatttaaa agagttgatg tagaaaaagg tagttttgaa tcatttctta
    20761 ggaatactaa gctaaaaaat tatggagaga aagtaaagta ttatgatgga agaactaagg
    20821 aatcaaaagg tatttatgat agtgtatttg atgttgatat tggcaataga gatttgcatc
    20881 aatgtgcaga tgcaattatg cttatgagag cagaatactt gtatcagaat aaactatatg
    20941 atgaaataag ttttgatttt gttgatggtt ttaaggcgaa gtattcaaaa tgggcacaag
    21001 gatatagaat ttcagtaaaa gatagtggtt caagctggta taaagcaact gaatattcaa
    21061 catcttatga gagttttaga aagtttatgg atatagtgtt tgcatattca ggaacgttat
    21121 ctcttgaaaa agaattggag cctgtaaaag ttgaggatat gaaaatagga gatgtgttta
    21181 tagttggtgg aagtccagga catgcaagta taattatgga tatggcagaa aatgaaaaga
    21241 ctggtgaaaa ggtatttatg atagcccaaa gctatatgcc tgcacaacaa acgcaacttc
    21301 ttattaatcg taaaaatcca aatttgagtc cttggtatag tttagatttt gaaggtgatt
    21361 tgacaatacc tcaatggact tttaaaagag accagttgaa aagattttaa aataataatt
    21421 ttatataata aaagaaaaat atagatgtga aaaaatgtaa ttagattaaa actaattgga
    21481 tatggacaaa attaaattga tagataaata ttaaaaatta cactaattaa tcttactata
    21541 gtttaatatt tatctaaaaa gttttatgta aaatataaat tttatattaa agctaaaact
    21601 aatttgagga ggctaattta tattgaagat tagaaggtat ttaattttgg taattataat
    21661 tctaattaca acaagtcaga gttttatttc ttatgggcta gagcgatttg aagaaataac
    21721 catttttcac actaatgata tacatggaag atatgctgaa ggtgatgacc atatacaaat
    21781 aggaaatcta gccacattga aaaaagaaac tccaaactca attttagtag atgcaggaga
    21841 ttgtttgcat ggacttccaa tagtaaatat ggatagaggg aaaagtgcaa ttgaattaat
    21901 taaatgtgct ggatatgact atattactcc tggaaatcat gactttaatt atggaaaaga
    21961 tagactgttt gagttatcta aaagtgctaa tttaggagag aatactttaa aatttttatc
    22021 atcaaatatt tttgaaaatg gaaaaaaagt ttttgatagt aatgatataa aagaaataga
    22081 tggaataaaa ataggatttt ttggactctc aacccaggaa acaaagagca aaactggatt
    22141 taaaaatgtg gaaggattag agtttaaaaa tcctatagaa agtgcaacag aacaagtaaa
    22201 tgaattaaaa ggaaagggtg ccaatgtaat agttgcaata tgtcatattg gtacaaatag
    22261 tttaagtaag ccaaatagta tagatatagc aaatgaggtt caaggaattg atttgattat
    22321 tgatggacac agccacacta agtttgaaaa tggtaaacaa gtgggtgata cattgattgt
    22381 aagtgcagga caatatttag aaaatattgg cgagataaaa ctaactttgg atactaatga
    22441 tgtagaaaat ataaaaatag aaaataaatc agttagatta attggaaaag aagaagcatt
    22501 aaagtataag cttgactccg ctgtaagtaa aaaggttgat gaaataaaag aacaacaaga
    22561 aaaaatatta gataaagtta taggggtaac tacaaatact ctagatggtt catatgaaaa
    22621 tgtaaggaca aaagaaacta atttgggtaa tttaataagt gacattttac tagataaaac
    22681 taaggctgat atttctttat ttaatggagg gaatattcga gatacgatag aaaaaggcaa
    22741 tataacaaga agagatattg tagatgtatt tccttttagc aatactatag ttaccaaaga
    22801 acttacagga gcacagatta aagatgtatt ggagcatgga gtaaaacttt atccagaaaa
    22861 aagttcagcc tttttgcaag tgggaggaat ttcatattac tttaatccta aacaaaaaga
    22921 aggcgaaaaa ataacaaata ttcaaaaaga aggaaagcca ttagatttaa ataaaaagta
    22981 tgtagttgca acgaatgatt atatagcatc aggtggagat gaatttcctt gtttttcaaa
    23041 agagccagta ctaaaggaat ttggaaattt agagagtgca gtcattgaat atatagagta
    23101 caaaaaagaa atatcaaaaa atgtagatgg aagaattggt attaaatcta aagatgagtt
    23161 aataagcaag aatcatgaga gtgatttgat attaagtgaa aatgaaaaaa atatcaatga
    23221 taatgaagag aatctatcta ataatataaa aagctctgat agtgataaaa ataaaagctt
    23281 agataaaaca agtgctagag aaagttctaa tctaggaata tctaaaagag aagctaaaga
    23341 aaaatctcct aaaacaggag atttaggttt ttcaaatagt ataattatat ttatagtttc
    23401 aagtacattg atatgtttat tgaattttaa tcaaaaagag ttaaaggata aaaagtcaaa
    23461 ataaaaatta atgagcaaaa attagtagat agtaatttaa ttttagataa tagtaaacag
    23521 caaaggaaat tagttatata aatactaatt tcctttgttt tatgttttaa catgattact
    23581 tttaaataaa attgtatttt atgaaaaata attacactaa atggcatagt attatgggga
    23641 tttttagttt ttattcaagt aaatgaaatc atattaacaa cttctgatat atttacaaat
    23701 tgataaaaaa atgattttat tagtatgata taatttagct agtatatatg atgatggagg
    23761 atatagggat gaataatata ttacttttgg aagatgacaa atctttaaat agaggaatta
    23821 gttttaaatt aaaaaaagaa ggatacaatg tttttagtgc atttggtata gaagaagcaa
    23881 aaaatatttt cgcaaaagaa gagatatgct taattatttc cgatataggt ctacctgatg
    23941 gtagtggttt tgacttttgt gaggagatta gaaagaaaag tgatgtttac ataataatgt
    24001 tgacagctct tgatgaagag gtggatattg tcacaggata tgatttaggt gcagatgatt
    24061 atatcacaaa gccttttagc ttgatggtat taatatcaaa agtgaatgcg cttatgaaaa
    24121 gagtaaatac agtaaaaaat tatactctat tagtttgtga tgatttattc ttttattata
    24181 tagaaaataa attaattata agaacagatg ataaggaaga agagataatt ttaagtaaga
    24241 cagagacaaa actattaaaa tatttgatgg aaaattctat gcagacattg actaaagagc
    24301 aactgttaga atctttatgg gatagtagtg gtaattttgt agatgataat acgattgcag
    24361 taaatataag aagacttaga caaaaggttg aaaaaaatcc atctgaacct aaatatataa
    24421 aaacagtaag aggggttgga tatatgtggg gagaaaggag tataaaaaaa tgctaaaaaa
    24481 tatgaacctc aaagatggtt taagtgtgaa agatgattat ataatgagaa gagttattgt
    24541 atattctgtt ttatctgtac taatagtaac ccttgtattt atactttttg aatatagcag
    24601 attacataaa ttgtatgaac atcaaataaa aaacactcaa aatattgctg gaatattgat
    24661 tgaaaaatat cctaatgatg aaatagatat agtaaattct atttataatt ctgattatag
    24721 taaggtgtct actggagaaa aggcatttaa gaaatttggc tatggattgg aaaataaaat
    24781 gtcaaatgat aaaaatttta gcttatactt aaaatctttt tttaaggaat cattttttat
    24841 ttttttaatg atgtttatag tagttatagg tataatatat tattttataa gatacataaa
    24901 taaaagattg agtaaaattt attttatagt agataagatg tcacaagaaa actacttgaa
    24961 ggacactact gagtcagaag aaactggtta tctagtagaa tataatgaat actttaaaga
    25021 aggcatattt agtaagataa ataactgtct atatgagctt aatagaagtt taaagataaa
    25081 gtttattaaa ttagaaaaag aaaaagaaag tgtaaaatca ttagtaacag atatttccca
    25141 tcaattaaaa actcctttag catccttaaa attatacaat acattactta tagaagaaga
    25201 attagaagaa gatgaagcca acgagttctt attaaccaat aaggcttcta taaataaatt
    25261 agaaaattta ataaattctt tagtgaacat atcaaggtta gaaattagca tgataaacat
    25321 aaaaaaagaa gataatgata taaaaaatac tattctaaat gcagtaaaaa gtgttacccc
    25381 taaggctaag agcaaaagta taacattaaa tttaaatgaa tttaacagta gaattatacc
    25441 acatgataaa aagtggactg aggagagtat ttttaatgta ttagacaatg ctgtaaagta
    25501 tacacaggta aatggagaaa taaatatatc tgtagaagaa actgctaatt tctttaaaat
    25561 tataatagag gataatggca tagggattga aaaaaatgag tttaataata tatttaaacg
    25621 tttttataga ggttctgctg atgaaattga atctgtagaa ggtagtgggg taggattgta
    25681 tttaagtaga aagattttag aagaacaagg tggaaatatt atagtttctt caaaagtagg
    25741 aatagggagc aagttttctt tgtttcttac aactatgtaa tattaaatga aaggaaaatg
    25801 taagcctcta catttattat ataagtataa acagctccag aaataaaaaa ttaataaaat
    25861 atggagtaat ataacttaga atgagaggat aattttatga aaatactata tactgaaaat
    25921 ttatcaaagc attatggaaa aggagaaagc ttagtaagag ctttggacaa tgttgactta
    25981 gaaataaatg aaggtgaatt tgttgcaata ataggaaaat caggttctgg aaaaagtaca
    26041 cttttgcata tgattggagg tcttgatata cctacatctg ggaaggtgta tatagataat
    26101 aaaaatatat ttacattaaa agaagaagaa cttgctgtat ttagaagaag aaaaataggg
    26161 tttatatttc aaagctataa tttaattcca tctcttaatg tatgggaaaa tgtagtgttg
    26221 ccaatcggtt tagatggtag agaagtagat gaatctttta taaaagaact tttaaaatca
    26281 ttaggacttg aaaataaaca cgacgtttta ccaaatacat tatcaggtgg tcaacaacaa
    26341 agggtggcaa tagcaagagc attagctaca agaccagcta taatacttgc tgatgaacca
    26401 actggaaacc tagattctaa aacgtctgat gaggttatga gtatattaaa atctatgtct
    26461 aagaagtaca gtcaaactct agttatgata actcatgatg acagtatagc ccaaatggct
    26521 gatagagtta tattcataga agatggcagg gtgtcaaagg ttggtgataa aaatgattaa
    26581 tactactaaa atagcaaaaa gtaatttaaa gcaaaataaa tcaaaaagta ttctaataat
    26641 aattactata atactttcaa ccttaatgct ttcttctatt ggaatttata ttgttgatgc
    26701 aggagcatat caaaaagaaa atactataaa gtattcaggc aattatcaag gtatattagc
    26761 aaatgtagat gaaaaacagg ctgatatatt gagtaatcat gcagatgttg aattaactgg
    26821 tgaaatgaat ggagtaggtg ttgaaaaact ggaagatgat tcaaatattt ctctagcata
    26881 catgaatgaa gatgctttga aattaaatag ttttgaattt atcaaaggaa aattgccatc
    26941 aaaagagaat gaaattgtgt tagatagtgg agctttaaag gcacttggtt ataaaaataa
    27001 agatttaggt gaaaagataa aaatatctta tgatgactat aaaaatgata aaaaaattga
    27061 aaaagagttc attataagtg gaatcttaaa aactagcgaa atatctgaag caggtaaata
    27121 ttattatgct attatatcag aatcatatat gagaaatact agagatatgt cacaagaaga
    27181 ttttaatatt tatgtgaaat ttaatgataa aagtaattta tcaatagagc aagcaaaaga
    27241 aaaattagat aaaattgcta atgatatagg actggatact attaatactg ctgtaaatga
    27301 aaattatata aatgctttaa aaccagatat ggaaacgatt atgggtggag tttttgtagg
    27361 gttagtaatt gtattatcta gtattttagt aatatacaat atattttata tttcaatagt
    27421 tacaaaggtt caagaatttg gaaaacttag agcaattggt gcaactaaga aacaaataaa
    27481 aaatatagtg tttaaagaag ggttcatatt agccggaata gctataccaa taggaataat
    27541 tttaggctat gtacttgcaa atattataat aaaatcattt atggatatag atgtaaaatc
    27601 atcacgatta ccagttatat tatcggttgt ggtaataagc tttatatctg ttgttttatc
    27661 acttttaaaa ccaatgaaag tagcatcaaa agtatccatt gttgacgcag taagatatac
    27721 aggaaataaa ataagtaata agagtaagag aaaaggatat aaaaatataa atctaaagag
    27781 attatcacat gccaatttag aaagaaataa aaaacgtact tacatgacat tggcatcact
    27841 tatacttagt ggaactatat ttataacagt atcaactgcc ttagaaagtt ttgatgctga
    27901 gaaaatggca agagaacact ttccttatga tatagaagtt agactatctg gttatgaaat
    27961 gaacagtgat aaaaatccta aagataattt gaatatttta caaatggata atcctctaag
    28021 taaagatttt tttaatcaaa taaagaacat ggaaggaatt aaaagaatag aatctgcaag
    28081 aagtgtaaaa attggtatgg aggactatga tgttgaattt aagtatgacc ttttacaaag
    28141 tataaatgaa aatgatgtaa aatcattaaa caagaatata atagatggaa aaataaatct
    28201 tgaaagactt caaactggag atgaaatagt aattactcat gttgatacag caaaagaaat
    28261 gggagtaaaa gcaggagata aaataagatt aactttatat gatggagata aaaagataaa
    28321 aaaagaattt aaagttcaag caatagctat gggggttcca agttttggta tagggaaaga
    28381 ttttatagat agaactttga aatacgattc aactagtagc ttgggaatat atacagaaga
    28441 aggaaagtat caagaaatca aagatagtat caagaagatt gctaaaaata atggattttt
    28501 agagactgat tttatagata gtagaattga gtcaaataag gctactattt catttataaa
    28561 gataatggga tacacgctaa caggcataat aggtgtgatt ggatttatga atcttgtcaa
    28621 cacaatgata actagcatag ttactagaaa aaaagaactt ggtatgcttc aagctattgg
    28681 tcttactaat aaacaattag tgaaaatgct taattcagag gcaatatatt acacttctgg
    28741 aatgatgata ggaagtattc tatttggagg tatattaggg tatattgctg ttatatttct
    28801 aaaaaagact ggattatctt atgcaacata ttctttgcca atagtaccaa tcttgttgat
    28861 gattgtgtgt attcttatag ctcaatttat aacaacttat ttgataggac gaagttttaa
    28921 taaagaatct cttattgata gagtaagata cagtgaataa aatgcaaata tttatggtta
    28981 gtttaactag caaaaaacat ataaaaaaat atagttaaag agattcataa ttaaataggt
    29041 taaagcatag tacagagcaa acaatttaaa cagatgtttg ctctgtattt tattatgtaa
    29101 tcaataatat atctaatata ctgatattat tcatgttaga aaatgtaccg gtacaattct
    29161 aaaaaagtat aaaattgtac catataaaat gaaaattttg tataataatc gtatagaaaa
    29221 taaataggag ctgatatata tgaataagaa gattaattat gcaccaggac caactgaaac
    29281 tagggaaaat gtaagattag caagagcaga aaaaacaaca aatccagata tagatattga
    29341 ttttgttgaa ttttataaaa agacatgcga gaagtttggt aatataattg gaacaaaaaa
    29401 cgatgtttac atattgagtg gtgaaggaat attaggtctt gaagcagcat gtgcatcact
    29461 tacagaaaaa ggagatagag tacttgtaat tgacaatggt atatatggag aaggttttaa
    29521 agattttgta acaatgtatg gtggggagta tgtgcttttt tcaagtgagt atactaaaag
    29581 tatagatatt gatgaattga aagcattttt agatgaggat agtaatttta aatatgcaac
    29641 agtagtgcat tgtgatactc caactggcgt attaaatgat gtaagcaaaa tatgtccatt
    29701 acttaaaaaa tatggaatat taactgtagt tgattctgta gcaggtatgg taggagaaag
    29761 attaagtgta gatgaatcta aaatagatat aatattaggt ggttcacaaa aagctatttc
    29821 tgcaccagca ggacttacta tagtcggaat aagtcaagat gcaaaaaatt gtataaaaaa
    29881 tagaaaaact gatgtaatag gtttttattg taatttaaat atatgggaag gctattatga
    29941 gaaaaaatac ttcccttata caatgccaat aagtgacata atggggcttg ataaagcact
    30001 tgataatata ttagaagaag gaatagaaaa tgtattaaat agacatgaaa agatagctta
    30061 tagtgttaga aaatctattg aggaatatgg tctagaacta tttttagaag aaggatattc
    30121 taatacagtc acagctataa aaattcctga aagtatagga gctttagaac ttactgatta
    30181 tatgcttaaa aattataata cacttgttgc aacatcacta aatcaatata tggatactat
    30241 tttaagaata ggtcatatgg gagagaatgc aaatctaaat aaaatagaac atgtactaaa
    30301 tgttttggat aaaagtttaa gtgcattagg attcaaagga aatggaaatt tgttaaattt
    30361 atttaataag tattattttt aatagatagg gggatatagt atggaagtta cagctaagca
    30421 aaaatttaca acgaaagaca tggtggaaac ctcactatta atagcattag tatttattgc
    30481 aactaaattt ataaacataa gattaccaat atctataaat ggtgggcttg tgcatttagg
    30541 aactgcaatg ttgttcatat cagcaatagt atttggaggt aaaaaaggtg ctttatcagg
    30601 ggctattggt atgtcacttt ttgatttgat atctgggtgg actttatggg caccgtttac
    30661 ctttatagtt agaggagtta tgggttattt acttggaaag attgcatggg caaatggtaa
    30721 taatgggaat aattttttaa tcaatgtaat aggaatatgt gtttcatcaa tttggatgct
    30781 atttggatat tatgtaacag aagttatact ttatggtaat tttatagtac ctcttacttc
    30841 aataccaggt aatttaatgc aagtattgat aggtcttata atagcgcttc ctatttctaa
    30901 ggtattgaaa aaatgtataa aataaatata aagtgaacta aaatttatga tagcataaat
    30961 tatactgtat aatattagga tggagtacat aattaaaaaa ttttagtata gaggttagtt
    31021 cacaatgtta tcagttacaa gatattcaat caaaaatgtt cgtttaaagc cgtgggtaaa
    31081 gtttatttgg tattttgaga caaaatcaaa tatactgtta aataataagt tacttccgac
    31141 agacagtata gatattattc tcaatctttc tgatgttatg gaatataaaa ttgaaaatca
    31201 agactatact gctagtaata tgcattttaa tggaataagg gataaacatg gatttattat
    31261 tcaacatgga aatatacgtg ttatagggat ttctttttat ccatttggtc tctatccatt
    31321 tttaaagata ccaatttcag agtttaacag acaaattgtt gacctagaag cagtatcaca
    31381 gctatttgca aaaaaattgg aagagtcatt aaatccaaca caatcagtag aaaaaattgt
    31441 tttatattta gaagaagtat tattatccat attagaagaa gatttaattt caaataaata
    31501 tgtaaagctt ctaaattcat ttatatatgt taatagatat agcaatataa aagcattttg
    31561 tgatgataca aatataaata ttaaaacatt ggaaagaatc tgtttaaaat atacagggta
    31621 tacacctaag attttaaaga gaatttacag atttaaaatg gcaagcaatc aacttattta
    31681 taattataaa gatgatgaac tttttgattt tatttatgaa aatgagtact atgaccaagc
    31741 acactttata aaagaattta aaaaattttc agggacgtca ccaattaaat ttattggtga
    31801 gaataagacg attaaagaaa atataaagta cagctattta taattatact gtcgattttt
    31861 tacaatacct ccaatttatt ttttgatatg atataggaaa ataaattgga ggttttacat
    31921 atgaaaatta ttacagaaat agttgagttt aatgtggaag aaaatttatc aaaagaagag
    31981 tttatagaaa ttgtaaatga cttagaatta aaatttcatt ctattcaatc tggttttatt
    32041 gatactgagc ttttatatga tgaaaagtct caaaagtgga ttatgataca acattgggaa
    32101 tctatagaag acctcaagag tgcttctagt aaaatgttta aagattcttc aactgagaaa
    32161 tttagaaaag ctttaaagaa tcaaactgtt aagatgaata taataccaca aattaaaagt
    32221 tggaaattat agaacttaat gtaaaagata gttaaatagt atcaaactaa attagccaaa
    32281 taacattttg tatttaaatt gttatttggc ttttagtgtt tattttaata tgagtgttat
    32341 atactagaaa aaccaacctt aaatactact atctaaaagc actattgctg tacgagaggc
    32401 aataggcatt ttagaagaag gaggagttga gctaaagtat acatcataat ctcttaagtt
    32461 ttcttttttt gtgtttcctc caaactctac catatcagta tttaatatcg ttttgtagtt
    32521 tgtattttga aactttgaaa tatcaactga ttgtattaga ttagggtgga aattaaatat
    32581 aaataaatat ttgcccttac tgtacaccaa aatcttttta tcattatcca tatgtataag
    32641 ttcatgacta ttttggataa aaatatcact gtattttgta agttcgagca tagatttatc
    32701 aaaatttaat aattgtttat actttaagtt gttatcttct gaaaggctcc attgtcttct
    32761 ggcatactta tagctccaat tgttgccttc tcgtggaaaa tcaatccact caggatgtcc
    32821 aaattcattt cccataaaat ttagatatcc ttcacctgct agagaaaaag ttattaattt
    32881 aattaattta ataagagata ttgctctatt gataacatgg ttatttgagt ttatttccat
    32941 attccagtac atttccttgt cagctagcca aaaaataatt gttttatcgc caactaatgc
    33001 ttggtcatgg gattcacaat atcctatatt tttttctcca ggtcgtctag tagtaagttc
    33061 ataccacatt tttcccaaat cccagtcttc atcagataag ttagagatag tttttatcca
    33121 aaaatcaggt actcccattg cgagtctata gtcaaatcca attccgccgt cttttatagg
    33181 gatacacata ccaggcattc cactcatatc ttctgcaata cttatcgaat taggtttaat
    33241 ttcttttatt aattcatttg caaattggag atatgttata gcttcaatat ctgtattcat
    33301 actaaaatat ttttcataac tatcaaatga aactccaaga ccatggttat gatataacat
    33361 tgaagtcaca ccatcaaacc taaagccatc aaaatggtac tcatttaacc aaaattttat
    33421 atttgaaagc aaaaaatgta ttacttcagg tttaccatag ttaaaaagtt ttgtacccca
    33481 agcaggatga tttccctttg aacctgagtg gaaaaattga tgttcacttc catcaaactc
    33541 atttattcct tctaaagtat tctttacagc atgagaatga actaaatcca ataaaacagc
    33601 aatccccatt gaatgagcag tgtttatgag gttttttaag tcttcaggag taccaaacct
    33661 agatgagatt gcataaaagt ttgaaacttg gtatccaaaa gatgcataat atggatgttc
    33721 cataatagcc ataagctgaa tggtattgta tccagctttt tttatcttag gaagtatttt
    33781 ttcagtaaat tcattataag ttccaataga ttcgctttca gtagacatac ctatatgaca
    33841 ttcatagatt agtggataag ttatgttttt taagtcaaaa tcattgtcag tccagataaa
    33901 tggagttttt ggttgccata tttgtccatt aaatccacca aaatcttttt gaattactct
    33961 ttttatatat aatggtatac ggtcaaatgt ttttccattt gcagtaactt gtacctttac
    34021 ctctgatttg tgaggtaagc tattttgacc aggaataaat atttcccaat taccagaccc
    34081 aatttttctt agaggatgag attttctatt ccaattatta aagtcaccaa ttagtgacaa
    34141 actatctgca tttggagccc attccctata gaagaatcca tcatttgtct gatgaaatcc
    34201 atagtaaaga tgagcatttg caaaagaact aaaatcttta tagtttttca atgtttttga
    34261 tttcatattc tcataatgtt tcattcgctt ttttatatca gattcaaatg gttgtaggta
    34321 tacatcaata tctaaaattt taaatctttt ttgacttttc attgtatttc tcctccataa
    34381 taaatattac atctaaaaaa taatatttat ctcatactat cttctctata taattgatat
    34441 atattatata atcctaataa aaagcttaag ccaccaatga ttaatttcat aatttacctc
    34501 cctagtattt atacttatat atcgtattca tcaaaattta aaagtttgtt taaaactctt
    34561 ttaataatat cataatcaaa gcctttatag gaaagatgtt gagaaatttt ttgatatatt
    34621 ttctttttat cttctttctt tactctttca tacctttttt ttgctagata catagcattt
    34681 tcaaattcag tgtccttgtc taattcagat actgcttcat tgattgtact tctattgata
    34741 cctttattat ataaattctg cttaattctg tttttgccac atttatttag gttaacattt
    34801 gtgttaacaa ttttttgagc taatacactg tcatttacga gattataact tttaagaaaa
    34861 tctatgaccc tctcaattat attttcatca aattctgatg atagtttttc ttttaatttt
    34921 ttttcagatt ggtcagcatg agataaaata tttaatgctt tatttttagc ttttatatac
    34981 atttcatcat ctaaaatagg ttttaattct tcttcattta tttcattccc cttttttaaa
    35041 ttaaatgtat aaacaagttc tttaaatata gcaataaaaa atttatcatc tacataaata
    35101 ttaactctat catcatttct tttttgttgt tctatttttg taattatacc cataagtcct
    35161 c
//
LOCUS       DAMJWS010000036        35092 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMJWS010000036 DAMJWS010000000
VERSION     DAMJWS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.036"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            38..214
                     /locus_tag="PC399_001876"
     CDS             38..214
                     /locus_tag="PC399_001876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592736.1"
                     /translation="MNLKKKEKIVKPVLKKLTEIVLNILSKKILNYAIYAISVLIVTK
                     VVINIDLYISILYY"
     gene            315..1052
                     /gene="cas6"
                     /locus_tag="PC399_001877"
     CDS             315..1052
                     /gene="cas6"
                     /locus_tag="PC399_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2592737.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            1057..2532
                     /locus_tag="PC399_001878"
     CDS             1057..2532
                     /locus_tag="PC399_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592738.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFCELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMEKNVKRIVDIVIKSN
                     IEIDEIDKLDLKQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRIDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     MNTRNLGKKMINNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGREEYKMKNYIESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2552..3568
                     /gene="cas7i"
                     /locus_tag="PC399_001879"
     CDS             2552..3568
                     /gene="cas7i"
                     /locus_tag="PC399_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2592739.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYRDKVHA
                     KTSIATITYDLRRILHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKEKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEREENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEEDGKLLLPEERKFMSKELREK
                     AVKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGV
                     IETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMW
                     YLKE"
     gene            3584..4381
                     /gene="cas5"
                     /locus_tag="PC399_001880"
     CDS             3584..4381
                     /gene="cas5"
                     /locus_tag="PC399_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2592740.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4408..6816
                     /locus_tag="PC399_001881"
     CDS             4408..6816
                     /locus_tag="PC399_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2592741.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7258..8205
                     /gene="prmA"
                     /locus_tag="PC399_001882"
     CDS             7258..8205
                     /gene="prmA"
                     /locus_tag="PC399_001882"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF2592742.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            8224..8982
                     /locus_tag="PC399_001883"
     CDS             8224..8982
                     /locus_tag="PC399_001883"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF2592743.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8984..10282
                     /gene="mtaB"
                     /locus_tag="PC399_001884"
     CDS             8984..10282
                     /gene="mtaB"
                     /locus_tag="PC399_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF2592744.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10325..10675
                     /locus_tag="PC399_001885"
     CDS             10325..10675
                     /locus_tag="PC399_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF2592745.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10838..11017
                     /locus_tag="PC399_001886"
     CDS             10838..11017
                     /locus_tag="PC399_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF2592746.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            11047..11490
                     /locus_tag="PC399_001887"
     CDS             11047..11490
                     /locus_tag="PC399_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF2592747.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11601..12098
                     /locus_tag="PC399_001888"
     CDS             11601..12098
                     /locus_tag="PC399_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF2592748.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12291..12854
                     /gene="raiA"
                     /locus_tag="PC399_001889"
     CDS             12291..12854
                     /gene="raiA"
                     /locus_tag="PC399_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2592749.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            13067..13303
                     /locus_tag="PC399_001890"
     CDS             13067..13303
                     /locus_tag="PC399_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF2592750.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13316..14659
                     /gene="yqfD"
                     /locus_tag="PC399_001891"
     CDS             13316..14659
                     /gene="yqfD"
                     /locus_tag="PC399_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF2592751.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14598..15713
                     /locus_tag="PC399_001892"
     CDS             14598..15713
                     /locus_tag="PC399_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF2592752.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15770..16231
                     /gene="ybeY"
                     /locus_tag="PC399_001893"
     CDS             15770..16231
                     /gene="ybeY"
                     /locus_tag="PC399_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF2592753.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            16237..16956
                     /locus_tag="PC399_001894"
     CDS             16237..16956
                     /locus_tag="PC399_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF2592754.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            17075..17476
                     /locus_tag="PC399_001895"
     CDS             17075..17476
                     /locus_tag="PC399_001895"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2592755.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17540..18433
                     /gene="era"
                     /locus_tag="PC399_001896"
     CDS             17540..18433
                     /gene="era"
                     /locus_tag="PC399_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF2592756.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18445..19824
                     /gene="mgtE"
                     /locus_tag="PC399_001897"
     CDS             18445..19824
                     /gene="mgtE"
                     /locus_tag="PC399_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF2592757.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19897..20052
                     /locus_tag="PC399_001898"
     CDS             19897..20052
                     /locus_tag="PC399_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF2592758.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            20018..20791
                     /gene="recO"
                     /locus_tag="PC399_001899"
     CDS             20018..20791
                     /gene="recO"
                     /locus_tag="PC399_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF2592759.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20784..21383
                     /locus_tag="PC399_001900"
     CDS             20784..21383
                     /locus_tag="PC399_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF2592760.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21543..22421
                     /gene="glyQ"
                     /locus_tag="PC399_001901"
     CDS             21543..22421
                     /gene="glyQ"
                     /locus_tag="PC399_001901"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF2592761.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22421..24487
                     /locus_tag="PC399_001902"
     CDS             22421..24487
                     /locus_tag="PC399_001902"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF2592762.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKILIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(24653..26200)
                     /locus_tag="PC399_001903"
     CDS             complement(24653..26200)
                     /locus_tag="PC399_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF2592763.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            26480..27247
                     /locus_tag="PC399_001904"
     CDS             26480..27247
                     /locus_tag="PC399_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2592764.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27530..27727
                     /locus_tag="PC399_001905"
     CDS             27530..27727
                     /locus_tag="PC399_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2592765.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27756..28832
                     /gene="vorB"
                     /locus_tag="PC399_001906"
     CDS             27756..28832
                     /gene="vorB"
                     /locus_tag="PC399_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2592766.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28835..29554
                     /locus_tag="PC399_001907"
     CDS             28835..29554
                     /locus_tag="PC399_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2592767.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29567..30106
                     /locus_tag="PC399_001908"
     CDS             29567..30106
                     /locus_tag="PC399_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2592768.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            30263..31321
                     /gene="buk"
                     /locus_tag="PC399_001909"
     CDS             30263..31321
                     /gene="buk"
                     /locus_tag="PC399_001909"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2592769.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31339..32232
                     /locus_tag="PC399_001910"
     CDS             31339..32232
                     /locus_tag="PC399_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2592770.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            32262..33470
                     /locus_tag="PC399_001911"
     CDS             32262..33470
                     /locus_tag="PC399_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2592771.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33494..34753
                     /locus_tag="PC399_001912"
     CDS             33494..34753
                     /locus_tag="PC399_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2592772.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        14185 a         3549 c         6477 g        10881 t
ORIGIN      
        1 gtttaataaa tataaaaaga gaaagaggga attttaaatg aatttgaaga aaaaagagaa
       61 aatagttaag cctgttttaa agaagcttac agaaatagta ttaaatatat tatcaaaaaa
      121 aatattaaac tatgctatat atgcaatttc tgttttgata gtaacaaaag ttgttataaa
      181 tatagatttg tacatatcca tattgtatta ttagatatct tatagatata aaaaattaat
      241 agatatatat ctattaattt tttatattac aaatagagaa taatgaatta tactagaaag
      301 taggaggtga aaatatgagc agaatttcaa tttttatgga atcatgtaat ggcaaatttc
      361 caaatgaaaa ttcaatgttg tcagtttcct ttataaaaaa tattttaaat gcagaaaaca
      421 aagattttgc taaaaacata tttaactatg gggaagaaag aaaaaacaat aagcaaataa
      481 aggatataaa tacagccatt tacataccaa atcttataag aagcaaaaat gagttacttg
      541 ttgatggaga tataatattc aatataagtt tttataatta ttctatgttc agtaaattat
      601 acaatggaat tttaaaagta aaggagctag aatataaagg ttatagattt aaaataaaaa
      661 atataaaaat acataaagaa cacgagataa aagaaaatgg tgttatattt aaaactatgt
      721 ctcctataat agtaaagaat aaagaaggta aatatttaga tgtagaagat tcaaattata
      781 tagaatgttt aaactatata gctaatctaa ctctggagag tataagaggt agtgggctta
      841 gaaagccatt ggaatttata cctcttaact tcaaaaaaag agtgttaaaa gaaaagattc
      901 gtggatttaa agagagagaa ttttactata taaatgctta tgcaggaaca ttttttttaa
      961 aaggaaatat ggaagattta aatgcactgt ataaaatggg aattggatat agaaggacgg
     1021 aaaatgcagg aatggttgat atattaaagt agcgaggtga gagaaataaa agctaaattt
     1081 tatagatcag gtgatattgt aagagatatt ggcattgttt acttttgtga attactaatg
     1141 gatttaaaaa acgaactaga aaaaaatgat tataagctaa aatttacttg tgagttgaat
     1201 agaaattatt tatctttaga atctgcagaa tatatagacc ctaagtatat atctgattat
     1261 atattagaaa atcaactttt taaggtattt aaaaatggga aaaaggaaac cttagaaaaa
     1321 atatttagta atatagataa tttaacatat gtaaatttta ttgaagaatt ggataaatct
     1381 tctgcaactg agaaacaaaa ggaagggatt aaaaaagatt ttaaaaacag aagatttcca
     1441 tatgttagaa atagtggtaa gtatggatta aacacgagtc tagaaaatat ggaaaaaaat
     1501 gtaaaaagaa tagtcgatat tgtgattaaa tcaaatatag agatagatga aatagataaa
     1561 cttgatttga aacaatatga agaaaaagat agtgtatgta atgtttgcaa tataaataaa
     1621 acaacaaaac tagatattga tttaagagaa cgaaaggact ctaagtataa ctttttattt
     1681 agaggtgcag aaaaatcagg ttttaaaaga agtggacaag ttgaaagtaa tatttgtttt
     1741 gagtgtgaat ttttcaactt aatgtgtctt ttatatataa atttaaaaag accaatggtt
     1801 tttgcatata cgaatgattt aagagaatta gcatttttaa atcataagat aatgcttaaa
     1861 agaaaaatgt attcagataa atcattttat aaaaaacttt tgcatgaaaa aataagttca
     1921 attagactat ataggattga tattgatacg aataaaggta taattctaaa atttgactct
     1981 attatagagt ataaagaatt attaaaaaaa attgaactta tagatattat agataattat
     2041 aatttttcaa gagagccaat gaatactaga aatttgggta aaaaaatgat taataatggg
     2101 aatttatcaa atttaaaaga attattactt gataatataa gtgttttaag gcaactaact
     2161 ggaacttctc gagaaatgga ttttgtatct agttcatata atatcggttt atatataaaa
     2221 ttgtgttgga ttgtagatgg aagggaggag tataaaatga aaaactatat agaaagtaat
     2281 ttaatatatt caagagtagg taaagactta tttaataaaa tgacagatga atccagaaga
     2341 aaaaattttt ctatgaaagt gattcaacta cttaagtcta atgatagaac tcagctattt
     2401 caaactataa tgcatgtgtt agtttctaat ggaatcttaa taggagaagg gtttgtagaa
     2461 ggtctaatgc agagttctga attagaatta aatacgaatg taggtctatt tattcaagaa
     2521 atcatgaaat aaaaaagggg gaaaatttaa aatgaatgaa aaaagagtaa aaggagtaac
     2581 attaactata ctgacagaaa gcccagttcc actttcgtat gaccaagggt atggaaatta
     2641 tacaccaata aaaaaagaac aatatagaga taaagtacat gcaaaaacta gtatagctac
     2701 aataacatat gatttaagaa gaatactcca tcaagaatat ggttggaatt taagtaatat
     2761 tgtttttgga aaaaaaggaa atatatatcc atctattaag aaaaatgtag gatgtgtaga
     2821 tgaaaatgga ttagagactg atgtatttgg ttatttaata ccacttaaag agaaaggttc
     2881 tataagtaag gcttctccgc ttagaataat accttttagg tcactaaatt catatagagg
     2941 ttcaacacag ttgataacaa atagaggttt tttaagtagc gaatttggta gaaaatatta
     3001 tgatgagagg gaagaaaatg aagtacttag agatgaaaac tttccaacaa cacaagcatt
     3061 agcaatagaa gagactttat cagattacta cgtttataca ataacattgg agcttgatag
     3121 aataggagta gtagaagtag aagaagatgg aaagttactt ttaccagaag aaagaaaatt
     3181 tatgagtaag gagttaagag aaaaagcagt aaaagatata ttagatgcaa ttactgtgtt
     3241 tacaagaaac ataaaacatt catcagtact attaaaacca cttgcagtaa tgggtggagc
     3301 ttttgataaa gttgtaccat ttttttggga tgatgtagat tataatgctg atagtggtga
     3361 gataaattta gagggagtca tagaaactat agaaagttat agccttaaag aaagtaacac
     3421 aatacttgct atcaatgaca gattaaaaat ttctaataaa aaggaattaa ataagtttaa
     3481 tttaagtaaa taccctgtaa aagagattaa aaatctagca gatagattgg aaattggaga
     3541 agataatatg tggtatttaa aagagtagga ggttttttga attatgaaag tattacaatg
     3601 taaacttaag caaccaactg ctcactatag agacccaaaa gtatttcaaa atgaatatat
     3661 aagtacttta aatttaccat ctaagaccac aataatggga atgataacgt atttatgtga
     3721 tagaagatta aactctgata tagatatagg aataattgga acacatcatc atagagaact
     3781 agaattttca agaggagaga acattgattt ttggaatgaa tatacaaata tgagaaaagg
     3841 caaagataaa gaaaagtttt tgcttcaagg aaactactat gattactata aagaacataa
     3901 agctcaaaat agcatattaa actatgaagt tcttaaagaa gtagaactga ctatatttat
     3961 ttcttgcaag gatgatgaag aacttgaatt tattagaaaa aagcttgaaa gtccatgtaa
     4021 atatgcaaat ttaggaagaa aagaagattt tgtaatacca agtgaaaaag ggtgttttgt
     4081 gaaagaagtt gagttaaaag aggttatacc tatgaatact cgtgatgcta taaaagaaaa
     4141 tataaaatta aagaatacat atgtaagagt tgacttaaga gataaagaga atgtcgagac
     4201 tataataaat caaggtgtat tgattgcatt accttataaa tataaagatt tagaagcaaa
     4261 tagagatgat agagtttatg agttttgcca ttatatatat gttgataatg atggtatata
     4321 tcctaaaaat attaaggtaa atgtatgtat tgatacaaaa gaagttttta catggcttta
     4381 aattattttg aaagataggt gtttatcatg aaaaatatca tgcaaaaaga aatattagct
     4441 aagtcatata atttaagaac taatcaacaa gagcaaacat tagttgaaca tattaaggat
     4501 ttatttgaag tattagaatc tatattagaa ctaaatctat attcagataa agatgtagag
     4561 atactaaaaa tatgttgtgc tttgcacgat ttaggaaaaa ttaattcaat tatgcaacaa
     4621 aaaatggaga ttaataataa aatatcgtat agttgctcag aagaagagag gaaaaagtta
     4681 gagtctgaca aaaaatcttt aaaaaagata gcaagacata atatattttc tggagcattt
     4741 ttaaaagata tattagaaaa aatgaacttg tcagaagaag ataagttata tatatataaa
     4801 agtataatgt tacatcatgg aaactatgaa gattatatga ggttaagtat tagtaaagtt
     4861 caagaagaaa tttatgagta tattgaaaaa ggcattttag aaaaagaaga gtttaatctg
     4921 aaagatatag aaagttatat aaatagcatt ttaaatgtag actttaaatt tggtgaagat
     4981 gtattggatt atgattacat agataaatta agtgaaagta tgtttataga cagtgattat
     5041 aacaatggtc aaatagatga ttcagtttta aattatagaa agtttaagta tattttatat
     5101 aaagggatgc tgaacttaat agaccatagt gcatcaagta gacagaaagg tataaaattt
     5161 tataacgatt ttactgatga agaaattgat aatatgattt taaatgagat atataagagt
     5221 cagggtaatt tggaaaaaaa taatatagag ttcaatacta tacaaaaaag gttgagagaa
     5281 ctttctggaa gaaatgttct gactgttgca tttacaggtt caggaaaaac agctgcagat
     5341 tatagaaaga cttttaaaag aaaattcttt ttagtgccaa ataaaatatc agcagaaagt
     5401 ttttatagaa aaaatatttt tcaaaataaa aatgatttag atacaagaag tagtaatgat
     5461 tacattggat tacttcatgg agatataaac ttatattcag agaatgaaga taatggagaa
     5521 catgattttg tattgacatt aagggatata gatttgagta taaacttttg taaaccttgt
     5581 gtaattgcaa cagttgacca gttattactt agtatgttta aatttccggg atatgaaaaa
     5641 atattcgcag ccgtaaaaaa tgcatctata actgttgatg aagtacattt attggaacca
     5701 aagatgtttt tgattttgat atattttatg cagtttgcat gcaagtactt agatgttgat
     5761 tttcacttaa tgacagctac acttccaaaa tcatataaag agcaaatgat aaacaaggga
     5821 attatttttc aagaagaaag taatgaaaat gttactgata aaggagaaat agtttttatt
     5881 gagagcaaca aggaagaaga gatttgtgaa ggtaaagatg taaaggtttc ttttataaag
     5941 gaaaaagaaa tcaagtcaat agttgaagat gctttagaaa ataaacaaaa aattcttata
     6001 ataaagaata ctattgattc agtcaataga acatatgagt ttttaaaaga aaatttatct
     6061 gataagtatg gtggtgtaga tatagatgtt ttacatagta gatttaagtt caaagataaa
     6121 aaagagaaat atagtaaaat attaaatgga gagggtgata tttggatatc aactcaatca
     6181 gtagaaatat ccttggattt ggatttcaat ataataataa gtgatttagc aactatggat
     6241 agcttgatac aaagaatggg tagatgtaat agaaataata aatatgaata tggaaacttt
     6301 tatattctac ctagtgaaga taaaatttat gataacaaac ttaagaacac aactaaaaac
     6361 atcttaaagg acatattaaa aactaactct atttttacaa tgggaattag aaaaactatt
     6421 ttggatgatt actatgataa tagtgtagtc aaaaaatatt ttgaagagaa ttttataagt
     6481 tgtgataaag aaattaagaa tatttatggt ataaataaag aaatttttga tggattggat
     6541 ttaattttta attttgaacc ttacaaaaat attgttgata gtaagaagga agcagctaaa
     6601 atttttagag atgttgatgt tagttacaaa attattctgg aagaagattt ttataaggaa
     6661 gatagagagt tacaacaaga ttcgattcaa gtatcagggt ttatatttaa tagattatat
     6721 tattatgggt taataagcaa agtagaagga tacatggtac taaaatctag tagtaagttt
     6781 gagtataata gtactgtagg cttaattttg aagtgagcat atgtttaaat ataatatacc
     6841 tttagacatt gaaaatacaa aaagtactac tgtaagctca ctgtaaaaac tattttttta
     6901 aaaggaaaaa taagttgtga gatagagtgt ttacaagtgt atatagataa taaatttgag
     6961 gtttaagatt aactaagtgg gtttcaggta catcgcgaaa ataattataa gttggagatt
     7021 ttgtatgttt aagattaact aagtggattt caaatattga tatagtagat ataatagaag
     7081 ttttattatt aggtttaaaa ttaactaatc ggatttttaa atattctgac tattatataa
     7141 ggatattatt agcttgaatt taaaaccaat aatataaaac aacaaaatat tatatttatg
     7201 atataataaa agttatggaa aaacaggaat attactatag agaagagagg taaacttatg
     7261 aataattgga tagaagtaac tataaaaaca acaacagaag cagttgaacc tataacgaat
     7321 atactatatg aacaaggagc tggaggagct gtaatagaag acccaaaaga ctttttattc
     7381 caaaagaaaa atgaactaga ctgggattat gttgaagaag aagtatttaa aaagaatgaa
     7441 gaagatgatg ttcttataaa aacttatgtt tctgaagaaa aaaatgtgat ggaatttgtc
     7501 gaaataataa agcaaaaagt tttagggtta aaagattttg gtatagatat aggtgaagga
     7561 agtgtatcat tggaccaagt aaatgaagct gattgggcaa atgcatggaa agcatactac
     7621 aaaccaacaa aagtaggaca aagggtagta gtaaagccta catgggaaga ttatgctatg
     7681 caagaggggg atttaatcat agagcttgac cctggaatgg catttggtac aggtacacat
     7741 gagactacta gtatgtgcat tagagaatta gaaaagtacg tgaataaaga ttctaaagta
     7801 tttgatattg gctgtggtag tggaatcctt gcaatagcag cagctaaatt aggagcaaaa
     7861 gaagttgtag ctgtagattt agatgaagtg gctgtaaaag ttgcaaaaga aaatgttctt
     7921 gaaaataaag ttgaaaaaag tgtatctgta atgcatggta atttaactga tgtaataaaa
     7981 gataaagcgg atgtaatagt tgcaaatata atagctgata taataaagat acttgcaaaa
     8041 gatgttcaaa actttatgaa agaagatgct atattcatat cttcaggtat aattttggat
     8101 aaagtagaag aagttaaaga aagcttaatt gaaaatggat ttgaaatagt tgaagttcaa
     8161 aaattaggtg aatggtcagc aatagtttct aagcttaaaa aataagggat atggtgacgt
     8221 ataatggata gattttttgt tgaaaaaaat aatataaatc tacaagataa aacttgcact
     8281 atagaaggtg aagatgtaaa acatatttca aaagttttaa gatgcaaatt aggtgagaaa
     8341 cttgaaatat gtgacaagaa taataatgag tatatttgtg agataatgaa tatagataag
     8401 agtatagtta atttagaaat tttagaaaaa gttgatataa atagagaatc agaacttaaa
     8461 gtaaggctat accagggact tccaaaagct ccaaaaatgg aaatgatact tcaaaaatta
     8521 actgaagtgg gtgtagaaga aataatatta gttcaaacta agagaagtgt tgttaaagtt
     8581 gatgacaaaa aagaagataa aaaatttgaa cgttgggaaa gaataattta tgaagcagca
     8641 aagcaaagca aaagaggtaa gataccaaaa ttaagaggtg ttttaagttt taaagaagct
     8701 ttagaagata tgaaaaaaaa taatgtaaat atttgccctt atgaaaatga aagaacagtt
     8761 tcaataaaac atgcactaaa aaaatgtgat tctaatatag atagtgttgg aatttttata
     8821 ggtccagaag gtggattttc tgaagaagaa atagaacaaa tacaaaaaaa taattgtaat
     8881 gttgtatcat tggggcctag aatattaaga actgagacag cttctgttgt agcatcaaca
     8941 atagctttgt atgaactaag tgatttagga ggagaaaaat aaattgaaaa aagtagcttt
     9001 ttatacactt gggtgtaaag ttaatcaata tgaaacagaa gctatgctag aactatttga
     9061 gaaagatggt tatgaacaag tcaatagtga agagtatgct gatgtttatg ttataaatac
     9121 atgtacagtt actcatatga gtgatagaaa atctcgtcaa tatataagac gagttaaaaa
     9181 gaagaatcca gatgcaataa tagccgttgt aggatgttat tctcaggtat ctccagaaga
     9241 aattttggat atagaagaag taaatttagt catgggcaca aatgatagaa gaaagattgt
     9301 tgaagaaatt aaaaagataa attcaagtaa gaaagttagt acagttgatg acataatgaa
     9361 agttaaagct tttgaagaaa tagaaatcag ccaaactaat ggaaagacta gagcatttat
     9421 gaaaatacaa gatggatgtg acagattttg tacttattgt atcattcctt atgcccgagg
     9481 acgcgttaga agtagagata ttgatagtat agtcgatgaa gtgaaaaaat tggctaataa
     9541 tggctataaa gaggttgtat taacaggaat acatgttgcc tcttatggaa aagatttaaa
     9601 ggatagggat attaaacttc ttgatgtaat aaaacaaata aaccagatag aaaaaataga
     9661 aagaattagg cttagttctg ttgaaccaat tttatttaca gatgaatttg taaatgaagt
     9721 tttaaagatg gataaagttt gtcctcatta ccatttatca cttcaaagtg gatgtgatga
     9781 aactctaaaa agaatgaata gacgatacac aactttagag tacaaaacta ttgtagatag
     9841 attgagaagt aaaatgccag atgtggcaat aactacagat gtaatagtag gttttcctgg
     9901 agagacaaat gaagagttta aaaaaacgta cgagttttta aaagaaatag agctttcaca
     9961 aatgcatatt tttaaatatt caccaagaaa aggaactcca gctgcaacta tggaaaatca
    10021 agttgaccct caaatgaaac actttagaag tgaacaactt ttaaatttaa gtaaagtaaa
    10081 ttttaataaa tttgcaacta aatttatcgg ccgtgagttg gatgttttat ttgaacaaaa
    10141 tatagaaggc aataaatatg agggattaac ttcaaattat ataagagttg ttgttgaaag
    10201 tgacaaaaat atacaaggtc agatattaaa agtgaaaata aatgatgtaa aagatgaata
    10261 tgtagaagga attttgctat aacatgtaga aattataata tttataagat aggaggtgga
    10321 aagaatggat tgtatttttt gtaaaatagc gaacggagaa ataccttcaa ctaaagttta
    10381 tgaagatgat agagtcttag cttttaatga cttgaaccca gttgctccat atcatatatt
    10441 agttgtacct aaaaaacatt atgatagttt aattgatatt cctgataaag agatggacat
    10501 tgtgtcacat attcatgttg ttattaataa aatagctaaa gagaaaggtt ttgaccaaac
    10561 tggattcaga gtcataaata attgtggaag tgatggaggg caagaagtta aacatttgca
    10621 ctatcatatt ttagctggta aaaagcttcc taattacgaa gcgggacaaa actaaactaa
    10681 attattgaaa aattgagttc cttcttgata aaaatacttg ataaaagtaa aaatttagtt
    10741 tataataaac aaagtgttag gttgtaatgc atattatgca aaaagaacat agttacagtc
    10801 ccagcataat tgcgctattg gagggaggga aaaagaaatg tcagaagtaa gagttagaga
    10861 gaatgaaaca ttagacagcg ctttaagaag attcaagcgt caatgtgcaa tgtctggcat
    10921 catgtcagaa gtgagaaaaa gagagcacta tgataagcca agcgttaagc gtaagaaaaa
    10981 agctgaagca gcaagaagaa aaaacgctaa gaaatagtag taaataagaa taaagaggtg
    11041 aaggggatgt cccttaagca aaagttacaa gaggatttga aatcttcgat gaaaaacaaa
    11101 gatacagtga gaaaatctgt agtaacttta ataagagctt ctataaagca gtatgaggtt
    11161 gataataggg tcgagcttga tgaagatgga attatcgatg taatagcaaa acaattaaag
    11221 caacgtagag atgcattagt tgaatttgaa aaagctggaa gagaagactt aataaaagaa
    11281 acagaaggtg aaattgaagt tttaaaagag tacctacctc aacaattaag cgaagaagag
    11341 ttagaagaaa ttgtaaaatc tactatatct gaagtagggg caacttctat gaaggatatg
    11401 ggaaaaataa tgtcagttat ccaaccaaaa gttaaaggaa gagctgacgg aaaacttata
    11461 aataagttgg taaaacaaaa cttacaatag attgatatag cctagagaga aatctctagg
    11521 ctttttatag tttttgggaa taacgagtca tatttgagca tatctataat caaagaaaat
    11581 tttgaaagaa ggggaaaaat ttgattatat cagtaaaaga gattaggaat tttttgataa
    11641 acatatttat accactaata ataggttact taagtagcat cttaacaatg attatcagtg
    11701 gaacagatat ttcaacctat tatttacaac tcacaaagcc aagctttgca ccaccagctt
    11761 ttatctttcc tatagtttgg actatattgt acatattgat gggtatatct tcatacctaa
    11821 tattaagaaa gggttacaac ttacctaaag taagagatgc aattttttat tatggattac
    11881 aacttgtatt aaactttatt tggagtatac tattttttgg atttggactt agatttacag
    11941 ctttgataga tataataata atgattttgg tttctataat aatgatttcg aagttttcta
    12001 aaatagataa gagatctgga tacataaatt tgatttattt aatgtggctt gtttatgcag
    12061 gatttttgaa ctattttata tggtttatta ataaatagaa tgtaaagatt ttgtaaatta
    12121 ccctgtactt ttaataatat cataacaaac gaaaaataaa tagactatta aaaataatca
    12181 ttttcatata caataatatt aatttaaata caataaattt ataaaaattg tatttaaaga
    12241 ttaacatatt aaagtattag aattggaaag gcttgaaggg agaggttttt atgcaaataa
    12301 tagtatctgg gagacaaatg aaattaactg acggaataaa aggatacgta gatggaaaat
    12361 taagtaggtt agaaaaatac cttgatcctg aatcagaagt gaaagtaaca gtaagtgcga
    12421 aaaaggatag acaaaaagtg gaggttacaa taattcctat taacggtcaa ataataagag
    12481 cagaagacgt agaagatgac ttatatgcag caattgatat tgtttgtgac aaattaagta
    12541 gacaagttgt caaatataaa actaaagtta aagacaaagt tcaaaataat aagagcataa
    12601 gatttgaaaa tcttgacttt atagataata gcagtgaatt tgatgattat gattatgatg
    12661 atgaagaaga tgagaatata gtaatagaaa gaagaaagaa atttaatgta aaacctatga
    12721 gttctgagga ggcgatactt caaatggaac tagtaggaca caatttctat atgtttagaa
    12781 accaagataa ttttgaaata aacatagtgt ataagcgtaa agctggtgga tatggtttaa
    12841 tagaacaaga ttaataaaaa ttgtatacgt ttagatttta tataagaaaa aataaaaagg
    12901 taatcatgtt gattttttat caatatgatt acctttttct tgtttaaata atatattaga
    12961 tattaggtat ttattatata aaaattttaa aactaatctg ttacaacatc aataaagtgc
    13021 ataaatacct atttcaagca taaaattaat taagggggta atgcttatgt tagaaatatc
    13081 cactgattta caagtaaatc aaccagttat tactgttaca agtaatacgt ttattagtat
    13141 agaaaattat ctatcaatat tagaatatga agttgattta ataagaataa agactaaagt
    13201 taaaacaatt aaaatatctg gggataaact ttcacttaag tatattactg atagtgagat
    13261 aggtataaaa gggattatct acaatgttga gtatgttgat taggaggaga tacacttgat
    13321 aagttttata aggggatact atgttatagt tgtggaagga gtaggactcg aacaattttt
    13381 aaatcatctt ataagaaatg ggataagtgt ttacaatgta actagaataa aaaatactaa
    13441 aatggagttt catatagata gacaagatat aaaagaattt aagaatgttt atagaggaag
    13501 taagtttgat ataaaggtaa agcaaaaaac tggggtccct tttattatca aaagagttta
    13561 taagcacaaa ggtatgtgga tttgtgctct tgtttcttta tttttattga tgtctacatc
    13621 acagtttgtg acagatgtat atatacaatc tcctgaaggt ataaaaaaag aagctcttag
    13681 aaatgagctt tataaggttg gagtaagacc aggagtctat aagaagagta ttgatagaaa
    13741 agaagttaga gaccatatga tgtcaaaatt taatgatgtg gcatatttat ctatcaatgt
    13801 aaaaggaaca aatatatttg ttactgttac aaaaaaggca gaatccctaa aatctacaga
    13861 ccaatcaaat tattgtaatg taattgcttt aaaaaatggt ataatagaaa aggtaatccc
    13921 tagaagtggt aaatcagtta ttaaatctgg agacatagtc caaaagggag atgtcttatt
    13981 aaatggagct aataccaagt caataccaga agtatgggca agtacttttt atgagtccac
    14041 aaaaaaggca agttatgtag atactgtaaa taaaaaaact ggagaaaaga aaaatatata
    14101 tacactaagt ttttatgata aagagtttac aatgagaaaa aatataaaat ataaagatta
    14161 tgttgttgaa aataaagaaa aaaaactatc aataggaaac tatacttttc caataaagat
    14221 aaaaacgagt actttttatg aaacaaaaaa ggttgaagta aaaaggaata aagaagagtt
    14281 aaaaaaagaa ttatctgaaa aagctttaaa agagttagaa tacataatcc cagcttcggc
    14341 tagaataata gatgtaaaac ataactttaa ggttaataaa aatatgttag aatatttaat
    14401 aacagttcaa actagtgaaa atattgctaa aatttatcct cttagtaagt cagaagcaga
    14461 aaggtttata aaggaagaaa gtaaaccaga tgaaggtgaa gaggaagttc cttccaatcc
    14521 agaaaaaaga ccattaaatg atataagaaa tgagtttgat gaggataata aggataataa
    14581 agatcaaaac aattctgatg aaaataatag taatcaaaat agtaataata gtcaaaataa
    14641 taatagcaat aacaactaat aaaattaaac ttaaaatctt aggaggacta attcaattga
    14701 taatacaaaa gaaatttaca atagcagatg gtaactttga aagagagtta tttggcaact
    14761 ttgatgaaaa tgtaaaatta atagagaaga ctttaaatat agatgtaata cttagagaag
    14821 gcaatataat tttaattgga gaagaaaaaa atgtagattc tgcattaaaa cttatgaatg
    14881 aattacacca aactgtatca aatggtaagc atttagataa gcagagtatt tcttattcat
    14941 tatctctttt gcttgaagga agtgaacaaa aaataaaaga acttgagggg acaatcgtca
    15001 taactcaaag gggaaaagct gttcaaccta aaactttagg tcaaaaagaa tatataaaac
    15061 ttatagaaaa taatgatata acttttggtg ttggacctgc tggaacagga aagacttatc
    15121 ttgcggttgc tatggcagta aaagctttta aaagagatga agttagtaga attatactta
    15181 caagaccagc tgtggaagca ggagagagtc taggtttttt accaggagat ttaaaggaca
    15241 aagttgaccc atatttaaga ccactatatg atgccttatt tgaaatgttg ggtgctgata
    15301 aatttaataa gtatttagaa agaggtacta ttgaggttgc accacttgca tttatgagag
    15361 gtagaacact tgataattca tttataatat tggatgaagc tcaaaatact acttctgaac
    15421 aaatgaaaat gtttttaact agacttggat ttggctctaa agctgtagtt acaggagatg
    15481 taactcaaac agatttacca caaaataaga agagtggtct aatacaagct acagagattc
    15541 ttaaaggtgt tccaggtatt gggtctataa tgctaacaga tagagatgtt gtaagacatg
    15601 agttggtaca aagaataata agagcttatg agaagcacga caaaagagaa gaatttaaaa
    15661 aagaagaacg taaaaaagcg aaaatgcagg aaaagacttt caaaagaaag taaaagtgta
    15721 gtaatataga tatatagcat tattaatgaa taaaaagtag gtgtagtgaa tggatttgat
    15781 actagatgat agacaagata aattagaagt aagtgaggag ttaattgaaa agataaagga
    15841 tataatcata gaatgtttag attatgaagg atatgatgat aattatgaag tgagtctttc
    15901 ttttgtagat aataaagaga ttcatgaatt aaatagagaa tatagaggag ttgatagagt
    15961 gactgatgtt ttatcatttc ctcttttaag tgatgatttt gaggatgttg agttagaaga
    16021 agaatcccta ggtgacatag tagtatctct tgaaagagcc ttagaacaga gtatagagta
    16081 taaccatagc tttgaaagag aagtatgttt tttaatatgt catagtatgt ttcatctttt
    16141 aggttatgac cacgatacag atgaaaatac aaaagaaatg agagaaaaag aagaacacat
    16201 attaaataag ttaaacataa caagggagta gtttttatga aaccagaaaa aactcgtcaa
    16261 ggtataatta aagcatttaa tgcagctata gaagggatat tgtatacatt taaatttgaa
    16321 agaaatatga aaatacatta tttaggttca gttgctgttc ttataataag tttgtttttt
    16381 aacttttcaa aattagaaat gataatgtta cttatgtcca tatgcttagt tgtagttgct
    16441 gaaatgttta atacagctat agaaaaagct gttgatttag taactgatga atatcatgtt
    16501 cttgctaaga tagcaaaaga tgttgcagct ggaggcgttc tagtggcagc actaaattca
    16561 gtagtagttg gatatatttt gttttatgat aaattgacag atatatctgg aatattaatt
    16621 tataaaataa gagaatcaga attacatata accttgatat gtatattatt ggttcttata
    16681 gctgttgtag ttgtaaaagc attgacttct acaggtactc ctctaaaagg aggaatgcca
    16741 agtggtcatg ctgccttagc atttgctata gcaactgcaa ttacacttat gactgaaaga
    16801 gttgttgcct ctactcttgc atatataatg gctgtattag ttgcccaaag tagaattgaa
    16861 ggaaaaatac ataccttttg ggagactatt gctggagctt tgttaggtgt gttgatagct
    16921 atattagtat ttcagcttgg aatgttctat aattaaaata aatgtacata aaatatttaa
    16981 aataaaacaa atctgtgcta attaaatttt tatgatataa tgtagagtat taaataaaaa
    17041 taacttttaa ttattgtata caggaggagt tgttgtggat aacatagaat tgttaaggtt
    17101 ggcagaagat gccagacaac attcttatgc tccttactca ggttttagag taggtgcagc
    17161 tcttttaact aagagtggta aagtttatac tggatgtaat atagaatgtg catcattagg
    17221 tggaacaaat tgtgcagaaa gaacagctat ttttaaagca atatcagagg gtgataagga
    17281 tatctataaa atagctatag caagtgataa ctcagaaaat aatgagcaaa catatccatg
    17341 tggaatatgc aggcaggtaa ttatagagtt tggttctgat ataaaaataa taacgggata
    17401 tactaaagga gaggtgtttg agcatagtat aaaggatttg ttgccaaact atttctctgg
    17461 aaatgattta aaataaattt aaaagtataa ataaatttaa gtaattaaaa caagaagata
    17521 aaaagttagg agacagttaa tgttcaaatc aggttttgtc agtatagtgg gtagaccaaa
    17581 tgtaggaaaa tctactttaa tgaataatgt agttggagaa aaaatagcta taatgagtga
    17641 taagcctcaa actacaagaa atacaataca agcagtatat acagatgaag aaatgcagat
    17701 agtattctta gatacaccag gtattcacaa accaaaaaat aaattaggtg aatttatggt
    17761 aaaggcagca acagaagctt ttaaaaatgt agatttaata ttatttgtag ttgatgattc
    17821 taaaaaaata ggtcctggag atagaaagat aatagaggat ttaagaagtg ttaagacacc
    17881 aataatttta gttgtaaaca aaatagacca actagaccaa aaagatgagc tatttgatat
    17941 aatcaaaatg tatgatagag aaggcatttt taaagaaata gtaccaatat cagcattaaa
    18001 aggaaagaat acggatacct taataaaagt tatacaaaat tatttagaag aagggccaaa
    18061 gtattttcca gattatatga ttacagacca gccagaaaga gtattaatag cagaacttat
    18121 aagagaaaaa gttcttcact atcttaatga tgaaatacct catggtgtag ctgttgaaat
    18181 tgaaaaaatg aaagcgagaa atgataagga aatagtagat gtatctgctg taatatactg
    18241 tgaaagagat tcacataagg gaataataat aggaaaaaat ggtaggaaac taaaaggaat
    18301 aggtaagtca gcaagacaag atatagaatt gcttttaggt tcacaaataa atcttcaatt
    18361 atgggttaaa gtcaaagaaa attggagaaa tttacaaaat tatataaata attttggata
    18421 taatgataaa taaaggtggg ttatatggat aggaaactgg atgcttccca agatttatta
    18481 tatgaagtaa aatcattaat tgataataat aaagtattgg agttaagaga attaatagag
    18541 gaatatcata taattgatat attcgatata atggaaaatt tagaggaaga catgaaaatt
    18601 caattatttg aagtacttcc tttagatatg gcatcttcta tattagaaga aggtagtgta
    18661 gagtttttta ttagtatttt atctaaactg gatgtagaac actcgaaaaa tatacttgaa
    18721 cttatgtctc ttggagatat ggcagacaag ttgagtgaac ttgaagaaga agaaagagaa
    18781 cacataataa atcttttgaa tcaagagaat gccgattatg taaaagagtt actattctat
    18841 gatgaagatt ctgctggtgg aacaatgacc actggatata tttctataaa taaagatatg
    18901 acagctcttg aggctataga ccatatgaga gaagaagctg aagaagctga aactatttat
    18961 tatatatatg tggttgatga tgaagaaaaa ctagttggag tattgtcatt aagagaatta
    19021 ataatagcta gagacgcaaa tatagtagaa gatttgatga gtgaaaatat aatctctgtt
    19081 tatgtagatg aagatagaga agaagcagta agattagtat ctaaatacaa tttaatagca
    19141 attccagtag tagacagaca agaaaaatta aaaggaataa taacagtaga tgatataatt
    19201 gatgtaatgg aagaagaagc aactgaagat atgtataaat ttgcaggaag ttcagaacat
    19261 gaaagagaag ttgctgaaaa agaaaaccct acattgagag agcaaataat atcagcactt
    19321 agaggacgat taccttggtt aattattacc ttagttggag ggttgttggc ttcgttaata
    19381 ctttctaact tagactatat aatgaatcct gtatatgctc cattagtatt ttttatacct
    19441 gtagtaattg gtatgggtgg aaatattgga actcaatcat catctgttac agtaataaca
    19501 ttatctaata aggatttaaa ttttagcaat gtggttagag aaggtattgt aggtattatt
    19561 acaggtcttt tgtgtagtat aataacaggt attgtaattt actttgttat gagaaagtta
    19621 gacattgtat taattgtttc aatatcatta tttataaata tggttttagg tgcaacaata
    19681 ggtgcgttta tgcctgtttt attaaaaaaa atggatgcag acccatctac agtttcatca
    19741 ccaattatat ctacagctct tgatataact ggtatagcag tttactttat aataacaacg
    19801 gcattattgt caaaaattgt ttaataactt tatgtatgat ttatcctctt cttactaaaa
    19861 atataagtat aaatgtaaga agggggtttt ttaaaaatga acaatatcag ttgggaagtt
    19921 tttaaaaaaa caggtagtat agatgcatat ttatatttct gtgattgcaa aaacatacat
    19981 gaagaaattg aagaagtaag ggagaaaaaa gaagacgatg ataatcctga acacccaggg
    20041 gatagtactt aaagctataa ggtataagga aagtgatata atacttacac tatttacaag
    20101 aaagcttgga aaagtatctg caatagcaaa aggagctaaa aaaaataaga gctctttgct
    20161 ttcatcatca cagttatttt cttatagcaa ttttacctta aaaaaacaag gtaatatgta
    20221 caaggttact caaagtgaaa taataaaaag tttttataat atatcctatg atatagaggc
    20281 attttcttat gctacatata ttacaaaatt agttgaaaat tctatacttg aaaatcagac
    20341 taataatagg ctatttatat tacttgcgca gactctgtat ttgtatacac aggataatac
    20401 agacaatagg tttataacag cagcattcga gttaaaattt ttagactata taggatttaa
    20461 acctatagta aataaatgca ttaattgtga atgtaaaatt ttacaaaatt ctgtgtttaa
    20521 tatctacgaa ggtggtatac tatgtagtaa gtgtagtaaa ctttttgata ataatataaa
    20581 attagactta acaactataa gtcttatgga atatgtatta agaaatgata ttttaacatg
    20641 tagtaaagct aaagtatcaa aatatataac acatgaactt gagaatattc tagacaagta
    20701 tttgaaagtt tatgttgata atattaattt taaatcatta cacgtattgc aaagtgtaaa
    20761 aaataataag ggagtggata atgatgagta gtaatataac tattgaaatg gttgattcag
    20821 tctttgaaag agttccagga gcaacttatg cagaagcaaa agaagcatta gtaatgtgca
    20881 atggagatgt aattgaggca gtaatatatt tagaaaataa aaaaaatact tgtaattgta
    20941 aagcaaaatc tgctaaagaa actatggaag aagtatttgg caaagatgga gaaaatataa
    21001 agtttcaatt aaaagagctt cttagaaaaa gtagtgtagt gagaataata gttgaaaaag
    21061 atggaaaaac tatgatgaat attcctttaa cagttggagt tgttggatta gcttttgtac
    21121 cattagctac tttagttgga ttatcagctg ctgttataag taaatatcgt ataaaagttc
    21181 aaaatgaaga agatggagaa attgtagatt taggtgaatt gaatgaagaa aaacttcacg
    21241 ttttgaagaa tatgataact aatgcagcaa aagatgtgaa ggatgtagta gttgacaaaa
    21301 aagaagatga caaagatgta actgctgacc ttatgaaaga agaatctgaa aagaataaag
    21361 aagacgaaga cgaaaataaa tagatataaa gtgactttta aaaaatgaag agaagataat
    21421 tataagaatt atcactaaca ttgacaaatt ttatagaatt tgatattcta aaagttgtag
    21481 aagaactatt aaaatagcct ttcgtgtaga cgagaggctt tattgttata ggagggattt
    21541 ttatgaattt tcaagagatg atactggcgt tgcaaaaata ctggtcaaag caaggttgta
    21601 tcatgatgca accttatgat attgaaaaag gtgcgggaac tatgaatcct aatacttttt
    21661 taagatcatt aggacctgag ccttggcaag tatgctatgt agagccttca agaagaccag
    21721 cagatggaag atatggtgaa aacccaaaca gactatatca acatcatcaa ttccaagtta
    21781 tactaaaacc ttctccagat aatatacaag aattatattt ggaaagtttg aaagagattg
    21841 gaattgaccc aagtgagcat gacataagat ttgttgaaga taactgggaa gctgctacag
    21901 ttggtgcttg gggacttgga tgggaagttt ggctagatgg tatggaaata actcaattta
    21961 cgtattttca acaagtaggt aatattgaat gtgagcttga gacaggagaa attacatatg
    22021 gattagaaag attagctatg tacattcaag aagttgatag tgtttatgat ttaaaatgga
    22081 atgataaaat aacttatggt gaagtattta ataaagctga gtatgaaaac tcaatgtacg
    22141 catttgaatt atgtgatgca gatatgttgt ttaatctgtt tgatatatat gaaaaagaag
    22201 cattaagact tatggaaaat ggccttgtaa taccgtcata tgactatgtg ttaaaatgct
    22261 ctcatgcatt taatacattg gatgcaagag gtgcaatagg agttagtcaa agagcttcat
    22321 ttataggtag agttagaaat atggcaaaga ctgttgcaga aacttttgta aaacaaagag
    22381 aagaaatggg attccctctt ttaaaggacg gtgacaaata atgaaaaatt atcttttatt
    22441 tgaaatcggt gttgaagaaa tgccatcaag atttgtaggc agtacactag aacaattaaa
    22501 aaacaatctt agtaaattat ttgatgaaaa tagaatagct tttgataaaa taaatgctta
    22561 tgggacacca agaagactta ctttagtagt cgaaggattg agtgatagac aaagtgattt
    22621 agaagaggaa ataaagggtc ctgctaaaaa aattgctgtt gatgcagaaa ataatttaac
    22681 aaaaccagca ttaggattta taaaaagcaa aggattaagc gaatctgaca tatattttaa
    22741 acaagttgga aaagaagaat atatatttgc gactataaag caagatggaa aagaaacttc
    22801 agaagtacta aaagacatat taccagagac tataaaatct gtagtattcc caaaagctat
    22861 gcgttgggga ggaaaaaatc ttaaatttgc aagacctata agatggatag ttgcattgtt
    22921 aaatgataaa gtattagagt ttgatttaga aggaataata tcttctaatg taacaaaagg
    22981 tcatagattt ttaggtgaaa gtacaataga agttgattct atagaagatt attttgaaaa
    23041 attagaaaaa aattatatag ttttagacca aaacaagaga aaagaaatga taagaaaaca
    23101 atgtatagaa gtagcaaatt ctttaggtgg agaagttgaa tttgatgaag atttacttga
    23161 agaggtaact tatttagttg aatatccaac tgctttttat ggagaatttg atgtggatta
    23221 tataaaacta ccaaaagaag ttgttataac tcctatgaaa caacatcaaa gatattttcc
    23281 agtacttaaa gaaggaaaac tgttacctaa ctttatagct gttagaaatg gtgattctta
    23341 cagaatagac aatgttaaag ctggaaatga aaaagtttta gaagcaagac ttgcagatgc
    23401 tttatttttc tatagagaag atactaagag aaacttagaa agctacatag aaaaattaaa
    23461 aactgttgta ttccaagtaa aattaggaac tatttacgac aaaactttaa gacttgaaaa
    23521 attaagtgct gatatacttg atagtttagg tttagtagat gaaaaggcag atactataag
    23581 ggctgctaaa ttatctaagg cagatttagt tactggaatg gtaggagaat ttgatgaatt
    23641 acaaggtttt atgggaaaag aatacgcaaa agttggagga gaaaatgatg ttgtaagtga
    23701 agctattttt gaacattatc ttccaagatt tgcaggagat atacttccta agactaatgc
    23761 aggggttgcc ttatcaatag ctgataagtt ggattcaata gcaggtttct ttgctatagg
    23821 aatacaacca acaggttctc aagacccata tgctttaaga agacaagctt taggtatact
    23881 tagtatactt atggacagaa aagttgctgt ggatataaaa atacttattg aacatgcttt
    23941 agacaattat tctgaattag attttaataa agaagaagtt gttgagcaaa taatgaattt
    24001 cttcaatgag agagtaaaaa atctatttaa agatttagga ataagatatg atgttgttga
    24061 tgctgtttta agttctgata taaatgatgt ttctgatatg catatgagag ctttagagct
    24121 taataattgg ctagaaaaag atgaattagt ggagatgctt actgcattta atagagtttc
    24181 tactctagct caaaaagcag aaagtgataa aattgatgaa tctttattaa aagaagatgc
    24241 tgaaattaaa ctttacaatg aatttaaaca agtaaaggta aaagttaatg aattattaaa
    24301 ggataagaaa tatggtattg ctttagatga atttgcatct ttaagaccag tagttgataa
    24361 tttatttgat acagttatgg tcatggataa tgatgaagct attaagaaca atagacttgc
    24421 tttacttaaa caagtatatg atactatgct aggaatttgt gatttatcta aaattgttta
    24481 taaataaaat ttaaactatt tataagaaat aaggctgtct caaaatagag atagtcttat
    24541 ttttcattgt tgagatgatg aaaatatatt tgatttcata gaaaaagagt gtctcataaa
    24601 ctaaaaagtt cattttgaga cacccatgtt ttttgtaagt aaatataata aattatattt
    24661 tatacttatc agttattttg tttaaaatat tatcatttga atttacccat tcataaaaat
    24721 tgcctgaaga tgcgtccgat attttataaa gcatttcagg aatatcacta gctttaaaat
    24781 ctccaagtga ttttcccaat ctagtcttac ctatttcaaa actgcctcca acaaataatt
    24841 caaatgcatc tgtagatata ccatcaacat tcttcttttt accacataaa cctatgctac
    24901 caacttgatg aacaccacat gaatttaaac atcctgatat atataactta ggtgctttat
    24961 ttaaaatttc ttcattattt tgtaatctaa aataatctat gatttcatgt aacattttct
    25021 gactattttg tatgcccatt tgacaaattg gaacacctat gcaagatata ctttgttcta
    25081 attgagaatt acaactaata gttttactta tttctaatat tctttttgct tcatttccat
    25141 ttaaatttaa aatatataat ccctctgtca ttcctagtct aatcatagga ttttcaacat
    25201 tatctaattc tttaagtaaa gtacttaaat cctttgttaa caaaatacct ccaactggat
    25261 gtatatatac agtgtataaa ccatcctgtt tttgaggtat tagtctagaa tcttgtatat
    25321 ctattttaac tccaggttta gagtaatcta tttcttcaat attgagttca agactaccat
    25381 tttctttttc taatttatag tattctttaa atttttctaa gaatagttct tcaccaagtg
    25441 attcgaccat atatctaacc ctagctctgt ttttattttc ataatttcca taatccataa
    25501 agaatttaat cataccttca acacaatata aggcgtcttt cggctttata agttcatcaa
    25561 gttctaaagc aactttgggt tgtttaccta gacctccacc taagaaaact ctgaaatatg
    25621 gttcatcatt tttcaaagta gcaacaaatc ctaagtcttg tatactacaa tgagcagtat
    25681 ctgtgtaaca attagaataa gaaactttta attttctagg taaatggtat gtagtagcat
    25741 ttttgataaa atatttatca gtagcaactg catatggagt aacatcaaag acttcactag
    25801 ggtcaactcc agataatgga gataatccga catttctagg ataattacca ccaccacctc
    25861 tagtgaatat atcatttttt attccttctt ccatgatatc aacaattgaa tttatagata
    25921 aatcgtgtaa ttgtatagct tgtctagttg ttaagtgtat cttatctaag ttgtgtttaa
    25981 tagctatttc atagatttta ttaagttgat actgagataa aactccagca gatactctaa
    26041 gtcttatcat aaaagatttt ttgtctcttt gagcataaac accatatcca ccagaaaatc
    26101 ccttatattg cattttattt atttctccat ttaaaaattt tgaagaatat tctttaaaat
    26161 taggtagttc atttaacaat atctttttaa aattatccat tatttcacct cattgttagt
    26221 cttgtataaa aatatatcta ttgtataata gatatatata ttattatcta gtcaggataa
    26281 aaatataaca gaataattct attaaaagta agaataataa ggacatagtg agtattttgt
    26341 ttttatttta caaattaagt ataatatttt tgttttataa aatataatta agataaatag
    26401 ataattttgt aattttaata tataatattt agtagagtgt gggagtagca cttaatattt
    26461 gcaggaggag tttttgtata tgggagaaat aataaatgca ttagatagag ccctagacat
    26521 aattttgctg ttatatcatg aaaagagaga aatgggaata actgaaataa gtaaggctat
    26581 gggagtatat aaaagtactg tacatagaac tttggttact cttgaaaaca aaggttttgt
    26641 tatacaaaat gcagagaatg gtaagtattg gctaggaata aatttatatg caataggtat
    26701 ggttgtggga gaaaaaatgt cattgactga aatagtaaaa ccatatacga aaaaattaaa
    26761 tcaagaattt aacgaagtag ttaatgtatc tatattagaa gaaagagctc aagattctcc
    26821 aagaagtatc ataatacata aagagtatgg ttcaaatcaa ttactttcag taaatccatc
    26881 cgttggttca agtagtgaat gttattgttc agcagttggt aaatgtctta tggcatttaa
    26941 tgattctata gattttgaaa aatatagaaa aacaccaata cacaagtata cagaacatac
    27001 aattgataat tgggatgata tgatgctttt tttagcaaaa ataaaagaac aaggatatgc
    27061 tatagatgat gaagaactag aacatggact aacttgtatt ggagcaccta tcttagataa
    27121 aaataataaa gctattgcag ccataagttt gtcaggtcca actataagaa tgagagaagg
    27181 ggactttgag tataagatta aaagagttat agaaactgca aaaagtatat ctgaactttt
    27241 tagatgattt ttaatctcga taataaaaat aggctatatt taaattgtta tttgtaaata
    27301 tcagtttaaa tgtagcctat ttttctttaa tctaaaatta aatattctta aaatttaaaa
    27361 tgttattgaa atatataagc gaatgtgtta tcttttaatt aagcaataaa aatagaacaa
    27421 ggttctatac tatagaacaa aattgatgca tatatagtat ttatacaaaa tataaatatt
    27481 atatatttaa aattaagtaa aaatattaca taaaggggag atttgagaaa tggaaaaagt
    27541 caagttgagt gttgaaagat gtaagggatg ctatctttgt attgaagctt gcaaaaaaga
    27601 tgcaataagt ctttctgact acacaaataa aaaaggcttt gtaactatta aagtcgatga
    27661 agaaaaatgt attcaatgtg gcgcttgtta tacaatgtgc cctgatttag tatttgagat
    27721 attataaatt atttttatta gaggggagaa aaggtatgca aagtttatta attaaaggaa
    27781 atgaagcttt agcagaaggt gctatacgtg gtggttgcag attttatgca ggttatccaa
    27841 taacgccaca atcagaaata ttagaaatga tggcatggag acaaaaagaa gttggaggag
    27901 tatttatcca aggtgaatca gaaatagcca gtgtaaatat ggttatggga gcaagtgcac
    27961 ttggagcaag agctatgact tcttcatctg gtccaggatt ttcattaaaa caagaaggta
    28021 ttgcatattg ggtatcagct ggtttgccag cagttgtagc atgtgtgcaa agatatggta
    28081 taggtgatgg atttatagga gctggtcaag ataattattg gcaagcagta aaaggcggtg
    28141 gaaatggaga ttatcattta atcgtgttag cacctaatag tgttcaagaa aatatggata
    28201 tggcatatga atcttttgaa ttggctgaaa aatatatgca tccagtactt atactttctg
    28261 atgctacaat aggtcaaatg gtagaacctt gtatagcacc accaatgaaa gaatacgata
    28321 tggataaagc tccatgggct gcaaaaggaa cacctgtagg agaaaaaaat aaagtaataa
    28381 ctgatataac atattttgaa gaccaagata catggcaaaa agggtatata gaaaaaatga
    28441 gaaaagttca agaagatgaa caaagatggg aagagataga aatagaagat gctgaagtgg
    28501 tatttgtggc atacggtatt tcatcaagaa tagcagaagg tgcagttaga gatgctagag
    28561 caaaaggaat gaaattaggt ctaataagac caaaaacatt gtggccttac ccaagaaaag
    28621 catttaagaa attaagtgat tcattgaagg gacttgtaac agttgaaatg aacatgatgg
    28681 cacagatgaa ggaagatgta attatagcat gtgataatag atttccagta tattcagtag
    28741 caacagctca atatatggca gaagtaaatc aattaataga ttttgctcaa cagattatag
    28801 atggaaaagc taagcaagag gaggtatttt aattatggct aaaaaagcac cagcaataat
    28861 gatgtgtgaa aatatgttct gtggaggctg tggacactct atagtcgata gaattattgg
    28921 agaagtatta acagaaatgg atatatgtca agatacaatt atatgttcag atataggttg
    28981 taatcatatg tttcaattta gtatgaatgt agatgttgtc gttccacctc atggtaaaat
    29041 gggagcagca atggcagcta tgaaaagagt tagaccagat aaatatatat ttacaatagc
    29101 tggagatggt ggagcttatg caatagggct tggagaaact atgtcagctg ctactagaaa
    29161 tgataatgta atatttcttg ttatgaacaa taatatgttt gcaatgacag gagggcaaat
    29221 ggctccaact actatagaag gtcaatatac agcttctact ccagcgggta gaaattacgc
    29281 agatcatggt tcaacatttg atgttgtaaa agttatggga aatttagata tagcatatct
    29341 agcaagagga acaataacta accctaaaga aataaataca acaaaaagat acatcaaaaa
    29401 agctctagaa aaacaaaaag aaaagaaagg tttttgctta atagaagtgc ttgtaccatg
    29461 tccaacaaac tggggattaa agccagtaga tgctatgaag agaattgaaa atgaacttgt
    29521 tccaaagtat ccacttggag aatttattga ttagggagga aataatatgt tgaatgaaat
    29581 tatatgtgct ggttttggag gacagggagt tcttacagca ggaaaaatta taatgtatgt
    29641 tgcatataag gcagaaaaga aaattacatg gttcccatct tatggaaatg agatgagggg
    29701 aggaagtgca aattgcaatg ttgttgtaag tgatgaaaaa attgcaaatc cttgtgtaaa
    29761 aaatccagat atactattgg gaatgaacag cgtatcagta gataagtatg aaagtttaat
    29821 gaaaaaaaat ggttatatgt ttgtcaatga atcactagta agtaaggata gaaagtacag
    29881 agaagacatt aatgttataa aagtacctgt tactgaaata gcacaaaatt taggcaatga
    29941 aaaagctgct aacatttgca tgttaggtgc tatggtaaag agtttggatt tatttacaaa
    30001 agaagaattt gaaagtggta tgtgtgaata ctttgaaaat caaggcaaag gtaaattcaa
    30061 ttctaaaaat gtagaagcat ttgaagctgg atacgactac gaatgaatat tttaaatatt
    30121 taaaataatt attgaaatac ttatttttat atgttagtat ttaattaata aaatatagaa
    30181 cgtcgttctg aacaatagga cgaagtcttt aggtcggaat acaagaacga cgttctgtat
    30241 agtagaacta gggggtgtta aattgaaagt tttagtaata aatcctggtg gtacatccac
    30301 aaaaatttct gttttccaag atgaaaatga aattttgaaa aaaaatataa cccatacaag
    30361 agaagattta aaaaattttt ctaaagtatt tgatgaatat gattatagaa aacaactgat
    30421 agtagatatt ttaagtagtg agaatcatag tataaattct tttgacgcag tagttggtcg
    30481 tggtggactt atgaaggcta taaaaggtgg tacctatact gtgagtgaag aaatgattga
    30541 ggatatgaga aatgaaataa atggggaaca tgcatcaaat ttaggtgcat tattagctaa
    30601 aactatagct gatgaaatag gagtacaatc atttgtagta gacccagttt ctgtagatga
    30661 atttgatgat gtatcgagga ttacaggaat atcagacata gaaaaagcaa gttggttaca
    30721 tgcattaaac cataaagctg tttcaagaaa aattgcacaa aaactagggg gaaaatacga
    30781 ggattataac tttattgtag ctcatttagg ttcaggaata tcgattgtag cacataaaca
    30841 aggcaaaatg attgatggaa gtggtggtag gtctgatggt ccattttctc cagaaaggag
    30901 tggtggatta cttgcatatc ctcttataga attgtgttac tccggaaaat atactcatga
    30961 tgaaatggtc gcaaaagtaa gttcgattgg gggaatgtac gattatttag gaacaaagga
    31021 tatgatagaa attgaaaaca gaatcaagga gggtgatgaa aaggcaaaac ttataatgga
    31081 tgcatttatt tatcaagtag caaaagaaat ttctatgtat ggagcttcac tttcagggaa
    31141 tgtagataga gttatcttga caggagggat atctcactca gaaatggtag tcaaaggagt
    31201 agcagaaaaa gtttcatatc tagctccaat agagatagta ccaggagaga tggaaatgga
    31261 ggcacttgct ctaggggcac ttagagtact taatggagaa gaaatagcaa aaacttatta
    31321 atatttagga gaaagagtat gttaaaagat tttaataatt tattagaaga ggctaaaaag
    31381 cttgaacctg ttattgtaag tgttgctgtt gcagaagaca aagaagtatt aaaggctgtg
    31441 aaatcagcaa cagatttaaa cattataaag ccaattcttg taggagacaa ggataaaata
    31501 gaaagtatat caagagaaat tggattaaaa ggtcatgaaa taataggttg tcaaaatccc
    31561 gatgaatcag ttaaaaaagc tgtagagata gttaaaaata aggaagctaa agttcttatg
    31621 aagggattag taaatacaag tgtctatatg agagctatat taaataaaga aacgggtctt
    31681 agaacaggaa gattattaag tttgcttgca gtatatgaat tacctcaata tcataaactt
    31741 ctttattgta ctgatagtgg aataaatgta tcgccaaact taaatcaaaa aaaagatata
    31801 atgacaaatg cacttcttac tatgaaaagt ataggcatgg aaaatcctaa aactgcgata
    31861 cttactgcaa atgaaatggt agaccctaag atacaatcta cagtggatgc caacctactt
    31921 gtagagatgg aaaaatcggg agaaatacca aagtgtatag tggaaggtcc aatagcattt
    31981 gatgtagcat ttgatgcaca tgcagcagaa cataaaggta ttaaaagtaa tattgctggt
    32041 gatgttgatt tattagtatt tccaaatata gaagcaggga atatattagg aaagtcatgg
    32101 cttagattta ataaagctaa gtgggcaggt atagttttag gagcaacaaa tccgataatt
    32161 ttaggttcac gttctgacac agctgaaata aaaataaatt caatagcatt agcgtgccta
    32221 gcatcacaat aagaaattat tattaaggag gaaaaagtat tatgaaatat gcaaaaagaa
    32281 tggatatggt aaaagcttca gctataagag attctcaaaa gaaaatagct cagaaggtag
    32341 caagtggtgg aactgttata tcttttgctg cagggcttcc tgacccaaat ttatttccaa
    32401 tagaagagat aagtgaaata actcaaaaag tacttaatga aaggggaaaa tttgccctag
    32461 catatggacc tactaaaggt gaagatgagt tgttagatct attagtaaaa agaatgaaag
    32521 aagaagaaaa tattgataca aaagcagaaa atattataat aactactggg tcacaacaag
    32581 ggatagcatt aagtgctatg attttattag aaaagggaga tgtagtagtt acagaaaatc
    32641 caagttactt aggtgcaata aatgcattta gaccttatga atgtgatttt ttaggagtag
    32701 atacagatga agaaggaatg gtaacagatg atttagacaa attattatct gaaaattcta
    32761 acataaaggt gatatatgta ataccaacat ttcaaaaccc tacaggaaaa acatggtctt
    32821 tacaaagaag aaaagatttt atggaagtcg tgaataaata tgatgtcata gtaatagagg
    32881 ataatcctta tggagaaata agatttacac cagaacctct acctacactt aaatctcttg
    32941 atactaaaga taaagtattg taccttgggt cattttcaaa ggtattatgt ccaggattta
    33001 gagttgcttg gatgtgtggt aatgtagaca tcatagataa ggcagaactt ttaaaacaag
    33061 gtatagattt acagtcaaat caattttcgc aagtacaagt aatagaattt ttaaagaaat
    33121 acaacttaaa tgaacatata gaaaaaataa gagcagagta taagaaaaga tgtctgctaa
    33181 tgttagatgc tatgaagaaa catttcccag aggaagctaa atatacagaa ccagatggag
    33241 gaatgtttat atgggttgaa cttcctgaaa gtataaatgt agatgattta ttagataaag
    33301 ccattgatgc tggagttgct tatgtttctg gagagtcctt ctttgcaaat aatggtccaa
    33361 aaaatactat gagacttaac tatacaacaa tgtcagaaga acaaatcgtt aaaggtgttg
    33421 agatacttgc agaagttata aaaaaagaac tcaattatgc ttgtgaataa atataaaaag
    33481 tgagggttta aatatgggag aaaaagaaaa cggtataaag tttggaaagt taatgtttca
    33541 acttgttatt atagcaatat cttgggaatt agtttatatt ataccattta tccaatatac
    33601 attatatgat ccaattctaa aagcattaga atgtacaaac actcaactag gttttctact
    33661 tacaatttat gggttaggta atatatttgg agctcctata ggaggctggt tagcagatag
    33721 atttgattat agaaagataa ttttaggttc tgtatttctt aatggagtag tatcattttt
    33781 atttgcattt aatatgaact atccatttgc agttgctact tgggtaggtt gtgcaatcac
    33841 atcattagtt atgaactatc cttctatggt aaaaatactt agagttatag gaaaagataa
    33901 tcaaggtaag gtttatggat ttaatgaagc tatggttgga gtttctggag tagtaatggg
    33961 agcaatattc ttatatattt atacatgctt tgcaactcct acattgggta tgagatgggt
    34021 aatgatatct ttaggaatat tatcaatagc aatgtgtcca gtattatggt ttgtaattaa
    34081 agatgtagat attaaagaag aaagagaaga agcaggtgaa aaaatgtctg ctggtgattt
    34141 tataacagta ctaaaatcac caaatacttg gctagtagga ataagtatat tctgtgtata
    34201 ttcattcaca gttacaatgt cttattttac accatatata acatctgtac ttggaggttc
    34261 tgtagcatta tctggtgcat tagcaataat aagacagcat ggtctaaaat tatttggagc
    34321 accatttgga ggatattgtg cagacaaatt aaaatctcct acaaaagtac ttttaccaat
    34381 atatgtattt ggtatagcag taatagtttt attcctagta ttacctgcat caactccaat
    34441 gactatattc atagctttaa catttgtagt aggtatatta ggttacatgg gaaaaggtat
    34501 atattatgca gtacaagatg aagtaaaagt tccagttaaa tattcagcaa ctactatagg
    34561 tatagctgca gctttaggat tttcaccaga tgtattccaa tttgcattaa taggacactg
    34621 gatagatact tatggcaata aaggttacac atatacattt atattccaaa tagcaattct
    34681 agttattgga atactatctt gtttatatat attaaaggtt aaaaagagaa gagcaagtaa
    34741 attagaagct taaatattca atttggtaat taagtatata taaatacaat acttattttg
    34801 atgaatgtga ttgtataaat aacataaaaa atatcaatag attaagtttt ataataatgt
    34861 ttagagaata aagtataaaa atcaaaatgt tatcaagttt tctaggtttt aacataaagt
    34921 tacccctgaa ggtgtaaaat tgagtggcaa ttattatcta ttttcttacc tacataaacc
    34981 tatttaattt aaattctaag agattttaaa aatagatggt agttgtactc aattttaaac
    35041 ctcataataa atacgaaaat ttaaaatcta actagatact atttaattat at
//
LOCUS       DAMJWS010000037        34740 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMJWS010000037 DAMJWS010000000
VERSION     DAMJWS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.037"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC399_001913"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC399_001913"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF2592773.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTDPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="PC399_001914"
     CDS             complement(2851..3216)
                     /locus_tag="PC399_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2592774.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="PC399_001915"
     CDS             3415..4263
                     /locus_tag="PC399_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592775.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="PC399_001916"
     CDS             complement(4700..5788)
                     /locus_tag="PC399_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592776.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="PC399_001917"
     CDS             5913..6641
                     /locus_tag="PC399_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2592777.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="PC399_001918"
     CDS             complement(6843..7334)
                     /locus_tag="PC399_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592778.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="PC399_001919"
     CDS             complement(7345..7788)
                     /locus_tag="PC399_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592779.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="PC399_001920"
     CDS             complement(7778..8617)
                     /locus_tag="PC399_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592780.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="PC399_001921"
     CDS             complement(8836..10221)
                     /locus_tag="PC399_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2592781.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="PC399_001922"
     CDS             complement(10394..11137)
                     /locus_tag="PC399_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2592782.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="PC399_001923"
     CDS             complement(11157..12419)
                     /locus_tag="PC399_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2592783.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="PC399_001924"
     CDS             complement(12603..13601)
                     /locus_tag="PC399_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2592784.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="PC399_001925"
     CDS             complement(13585..15096)
                     /locus_tag="PC399_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2592785.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="PC399_001926"
     CDS             complement(15150..16112)
                     /locus_tag="PC399_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2592786.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC399_001927"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC399_001927"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2592787.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18105)
                     /locus_tag="PC399_001928"
     CDS             complement(17092..18105)
                     /locus_tag="PC399_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2592788.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18333..19310)
                     /gene="bioB"
                     /locus_tag="PC399_001929"
     CDS             complement(18333..19310)
                     /gene="bioB"
                     /locus_tag="PC399_001929"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2592789.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19516..19779)
                     /locus_tag="PC399_001930"
     CDS             complement(19516..19779)
                     /locus_tag="PC399_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592790.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19849..20922)
                     /locus_tag="PC399_001931"
     CDS             complement(19849..20922)
                     /locus_tag="PC399_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2592791.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21181..21939)
                     /locus_tag="PC399_001932"
     CDS             complement(21181..21939)
                     /locus_tag="PC399_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2592792.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(21936..22853)
                     /locus_tag="PC399_001933"
     CDS             complement(21936..22853)
                     /locus_tag="PC399_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592793.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22853..23053)
                     /locus_tag="PC399_001934"
     CDS             complement(22853..23053)
                     /locus_tag="PC399_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2592794.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23058..24152)
                     /locus_tag="PC399_001935"
     CDS             complement(23058..24152)
                     /locus_tag="PC399_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592795.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24662..24802)
                     /locus_tag="PC399_001936"
     CDS             complement(24662..24802)
                     /locus_tag="PC399_001936"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2592796.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            complement(25112..26419)
                     /locus_tag="PC399_001937"
     CDS             complement(25112..26419)
                     /locus_tag="PC399_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2592797.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIVHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26484..26963)
                     /locus_tag="PC399_001938"
     CDS             complement(26484..26963)
                     /locus_tag="PC399_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2592798.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27010..27873)
                     /locus_tag="PC399_001939"
     CDS             complement(27010..27873)
                     /locus_tag="PC399_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2592799.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27873..28628)
                     /locus_tag="PC399_001940"
     CDS             complement(27873..28628)
                     /locus_tag="PC399_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2592800.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28655..29143)
                     /locus_tag="PC399_001941"
     CDS             complement(28655..29143)
                     /locus_tag="PC399_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2592801.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29161..29562)
                     /locus_tag="PC399_001942"
     CDS             complement(29161..29562)
                     /locus_tag="PC399_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2592802.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29565..30062)
                     /locus_tag="PC399_001943"
     CDS             complement(29565..30062)
                     /locus_tag="PC399_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2592803.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30086..30490)
                     /locus_tag="PC399_001944"
     CDS             complement(30086..30490)
                     /locus_tag="PC399_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2592804.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30720..33287)
                     /locus_tag="PC399_001945"
     CDS             complement(30720..33287)
                     /locus_tag="PC399_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2592805.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33453..34196
                     /locus_tag="PC399_001946"
     CDS             33453..34196
                     /locus_tag="PC399_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2592806.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT        11262 a         6360 c         3560 g        13558 t
ORIGIN      
        1 cttaaaaata attcgtcaat actttttaaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaacgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatacttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaaaaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggatctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcatgcc taacaatgtg cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggca tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctgtac aaggtatacc acaagctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtgttgg tgattcagaa
     4501 tagtatggta ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gcaaaaaaat
     6301 ataatgtaac cgattttcta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatgtttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagtttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaaaccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgatcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaat cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcatagttca aagaaaacct agagatttct ccatgatttc
     8521 tttttaagta tttacatagt ccacaatagt aagctttgta ataatcatat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttatgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttgttta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcctt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg tagctaacat ctccaatatc taatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg cacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg gtattcttat tggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcttct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta ccaagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttagta gtggatactt tttgagacaa
     9961 accatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccataact
    10021 agccacaaat gctgtatcca taccttctaa tccctctata ctaaaaccat gtttttttaa
    10081 acattctttt tgtaaatttg aagcatagta ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattatcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctacc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgttcag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccaaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaaccc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattac tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 ttttttccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gaccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacccgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatccacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattct cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaatat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcctcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcacc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattttgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatattt ttcaacatct tctttataag
    16201 gcataccaga ttgtgcacct agtttagtta cagacagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tgtttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattgcact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg tagccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgaa aaaaaaatta atatactttt ataattatct
    18301 aaaagtatat taattaattt aagcagtatt tattaaagtt caaatccttg ctctataatc
    18361 atttttttat cattttcaat cccacttcca acagttgtca aataatcacc aacaattgca
    18421 gaatttactc ccccattaaa tccaactttg tcatatccct ttaatgatag ccttccacca
    18481 gcatacctta tgtacacatt aggaattata aacctaaata ctgctgttgt ttttagtatt
    18541 tccattggtt ctaatacatc ataattttcc attggtgtat tcttaatagg atttaaaatg
    18601 tttattggaa atgattttac atttaattct cgtatttcaa aagccatttt tagtctctgt
    18661 tcttcactct cattcattcc aattataccg cctacacata catctagccc agctttttta
    18721 gcatctttta tagtttttat cctatcttta tatgtgtgtg ttgtacatat attatgaaaa
    18781 ttatctctac acgtctcaac attgtggtga tatgtagtca cacctgctga cttcaacatt
    18841 ttagcttgct cataatctat aatacctaaa gatgcacata attttaaatt agtattttcc
    18901 ctcaatcctt catagatatt taaaatatta ttaaactcct ttcctgacat acctttacca
    18961 cttgtaacca atgagaatct atgcactcct ttactctcca tctctttagc tctatttaag
    19021 atttcattgt aatctaataa tgaatattct tctacaccag ttttataata agaagactga
    19081 gcacaaaatt tacagtcttc actacatttg ccagatttag cattcattat cgtacataaa
    19141 tctgcttttc ttcccataaa atattctctt atttcatttg cagattttaa tagaatatca
    19201 aaatcgtttt tattatttgt atcaaggctt attaaattaa gagcttcttc ataacttatt
    19261 tctttttctc tcaatacttt attttttaat tttattatgt atttttccat atatcctcct
    19321 atttgtcaac ttaatatttt tatgtagttt acttattata tatagtattg catatataac
    19381 atttattggt caactttatt ttagacacaa gtttactata cacaaaaaaa tactgcttca
    19441 aaacaatatt agtatataga ttatacatat tattattaaa gcagtatttt ttattagaat
    19501 ataattctta ctggtttata aaactatcct tgtcttaact ccatccaagc tttccattgt
    19561 aataacttct aagccttcat attctctaaa tagccatttt aaatccttca tggcttgatt
    19621 aacctgactt tttgttagat tatttataaa atattctttt ctagtttcct catactcttt
    19681 gctagtcaaa aagtttctta gaaaattaaa cgcaaataca cttggaatat tgtttacatt
    19741 agccttcttg ctgttttcct tctcatagta aattttcata aatgcatact cctttgatac
    19801 ttttagattt ctcttacaat aattatatgc atttaagtgc tactattctt ataattttat
    19861 taattcatat tctttacttc ccatacccat ttcacatgca tagtctatta ttctagttcc
    19921 atttacatga ggatgttcat gtttgaaaac atcatgaccc atttccttac aaatcaaatc
    19981 atagcatgct tgttcaatag ctacagggtc tgttgatgct aatattccta tatcatgggc
    20041 aattggtcta cctgaccaag gtacacaatc acaaagtgga gttacattca taacaaaagt
    20101 tatataccct accttgtctt ttttatttga tactgctcca taagcgtatt ctgccatctt
    20161 ttcgacaaac acatctgagt cactttccca ttgtatagtt actgctctag ttggacagac
    20221 agttggacat tctccacatc cataacatac atcagaatct ataactgctt ttttatccac
    20281 tatacttata gcttttgtag gacaagaatt aacacacttt ccacaaccta cacacttctt
    20341 ttcctttact actggagtaa catcagaatg ttgcatttgt tttcctgctg cacttgcaca
    20401 tcccatagct aagtttttta atgctcctcc aaaacctgct gcttcatgac ctttaaaatg
    20461 actcattact atcattgcag aagaattata aatttctcca cctattttta cactttcaaa
    20521 atgttttttg tctattttta cattctcata atttctacta tataatccat ctgcaattat
    20581 tactggtgca tttacaactg cataagcaaa tccattttct atagcagttg taagatggtc
    20641 tacagaattt gttctactcc ctgtatacag tgtatttgta tctgttaaaa atggctttcc
    20701 ttcgcagtct ttaaccttat caaccacttg tctaactgcc acaggactca tatatgtagt
    20761 atttcccttt tctccaaagt gaagtttaat tgcgacttgg tcattttttt ctattaaatc
    20821 tttgaatcct gcttcatcaa ataatcttct tacattgttt ggtacgtttt tattttgcga
    20881 atttgagtat aaatcacaaa aatatacttt tgacttttcc atatctacac ccctttaaaa
    20941 tatttactta tactaatttt atcactttta aagtaatttt tagtatattt taacataatt
    21001 aaatattctt atatttattt gatacaaata atattatttg tattattttc tacacttaat
    21061 tctatatatt aaactacacc taaaaatcaa aaagacttga aacttaagtc caagtctttt
    21121 tgatttttag gtgtagtttt taacaacata agattacttt accttgtgat aaaggtatta
    21181 ttaaactggt tttttattaa ataaaataac agctgctagt aaacatacta tagaaataag
    21241 aactgttata ataattggaa taataaattc tggcattgcc actttccctg aaatcaagtc
    21301 tatatttttt gacatcaaga aattaggagt ataaggtgca atctgtttag gaatagaaat
    21361 taaactcaat aagagagttg ctaccaatac aagtaaaaca ctggcaaatg ctggtctaaa
    21421 tagtacacac ccaagaatta atacactaat atacataatt cctataagcc ataaattaaa
    21481 tgctgaaaat ataatatgat taagatttgc agttggccaa aaatacattg tatagccata
    21541 agcaaataaa aaactcaacc aaaaaccaat tgtcataata atcaccgtaa cagagaattt
    21601 agacaatact atagatgaac gtgataaacc tttagtcagc ataataacaa gtgtcccttt
    21661 agaatattca tttgataaaa tattacaaaa aataattaga gttaaactca taccaagacc
    21721 agagatattg ctaaaaaatt gtgtccaaga atctaaagca gttggtgcag cagtaacttt
    21781 taaagtcggt gcaaaatgag caatcaagtc tggcataaat tttgcagaaa taggactact
    21841 tataccaaat attagaaata aaataattaa actaaataat ttataatttc ttaaattctc
    21901 tttaaattct ttttttgtaa atgctatgta ttctctcact ttactacctc caaaaataaa
    21961 ttttcaagtg ataattctat tagttccatc tttactggac aaagattcat ttctgcgagc
    22021 gtagatataa ctgttttttg tattgctttt atatctttac catgcaaaac aatttccata
    22081 ttagtttctt ctgaatcttt tagtaaggat ttaataccat cactagactt aaacttttga
    22141 atttcatcat tacttgcaaa ctccagtaaa agtctatcct ttccgtgcat actttttatt
    22201 tctgaaagtg taccactaag ggcaatgctt cctttgttta atatagcaac atggtcacag
    22261 attctttcaa catctgatag tatatgtgtt gaaaatatta cagttgttga gtctttgatt
    22321 ttaagcataa tatctaatat ttcttttcga ccaacagggt caagtgcact tgttggttca
    22381 tcacaaataa gtaatttggg gcgtgaaagc aaagcttgag ctataccaag tcgttgcttc
    22441 attccacgag aaaacccacc tattcgtctc ttttcatttc taagacccac aagtgataac
    22501 aactcttcac tccttatctg tatttctttt tttgaaagtc ccgtaatttc accacaaaga
    22561 gaaagatatt caagtggtct catataattg taaaactctg gaacatcagg cagataacct
    22621 acatgacgat tagaacttgt tttcccatag gtcactttct caccacaaac atctatagtt
    22681 ccactatctg gtttaagtaa cccaagtacc attttcattg ttgtagtctt acctgcacca
    22741 ttcttaccta caaacccaaa gactgaccct tctggtacag taaaatttaa attatctatt
    22801 atctttcgat taccaaaacc ttttgataat ccatttattg agacaatatt catcattctt
    22861 cacctcttcc tattgtaaaa tacaaaattg gtcctattaa actaataaac agtactacaa
    22921 taatccatat tgccttattt ccaattttat aatttggatg tctcaacaca tgaacaagtg
    22981 cagtaattat aagtattaaa tctaaaataa taacaggaac caaaagtggt aagtattcca
    23041 tcaaattttc cataatctta ttcctccaat aaagataatt tttctataag aaatttgtat
    23101 tctttgtttt cacttaatac agaaaacatt ggattatttt taatagcagc aacaatacct
    23161 gcttttacga ttgtatcatc acgtgtaata cctgttccaa tttcagaaat ccatctgtca
    23221 agtttattaa aaaagtcatc cccatgtatt ataattgggt atatatcacg tgttgctaaa
    23281 tctacatatt ttcgtaatga tttaatagct ccttcattat caccatgtat taaattaatt
    23341 tgtgcctgtg ttaaatatga agaaagcata atagcaggag ataatgtatc aagctcaaat
    23401 atatctgaaa ttgctagaat acgtctttct atttccttca tttgtaattt atctgcattt
    23461 tgcaaaatac ccactagatt ttggactaat gatataaggt tctgatatgc accaagttga
    23521 aatgtttctc ttgcttcatc catttgacca ttcatcattt gcccttgtgc aagaagtata
    23581 gattcattta tcatgggaaa attgctgttt tgcaataaat caatcacttg tatgggttga
    23641 tttaacaata tataacaagt tgcctccata ctttttgctt gacggcaaat atctatgtca
    23701 ttacaagttt cctgtatacg aataaatatt tctaatgctt cactaattaa tagttcacgc
    23761 tttttttcat ctacaatatc atagtgatta actattaaaa gacccatccg aaaaatcaaa
    23821 ggaaaacatg aataatattt ttttatgatt tctcttattt catccatgac ttcatcaaat
    23881 ggtttaaccg taaagtcttt gcacagttta ttatacagtt tattaatatc ttccttcatc
    23941 atttgaggtt cataacatat taactcatca acggtaatgt tgaaatatgt cgcgagttgc
    24001 ggtaaaaagg ttatgtcagg atagctttgt ccagtttccc atttagatac agaagcttta
    24061 gaaacgccta tataatttgc aagttcatct tgtgtaattc ctttttcttt acgctttaaa
    24121 atcagcgttt ttgcgatgtt taattcattc atgtcaatcc ctccttatgt ataattatat
    24181 ccttaaacat gtaaagctaa caagcgattt gaagttgact tttgacgaaa aaatcaacca
    24241 cagagaaaca aataaacaag tatataaaat atatatttaa atttatcttt aatctccttt
    24301 gtaaaatatg taaactttgt aaataaaatc catattatat aaaactaata gttaatctat
    24361 ctaaatacag tttttattta tcacgttttt aaatattttg tgtaaaacct cttttaatat
    24421 aaacacctta ttttcatttc tcatttcata ttctttttaa aatactgaat ataactcttc
    24481 gattttccat ttaatccttt tataaagtta ttattcgtat ttattcctct aattataaca
    24541 ggcaactatc atgtattggt atttgtctat tatataaaaa tctataaaag aaaagcagtc
    24601 aatcatttag attaactgct tgtacatata tcttattaaa ctgtataaac tataattttt
    24661 attattctcc attttcatcc caagtcataa atccccatcc actatctgga taaatatcga
    24721 tagacttttc tctaatcata taaccaactt tataacactc ttttgttatt ttattagata
    24781 gtatacatcc ttctccattt atctaattcc atttaattaa cttttctttt caaccttaac
    24841 ttttataaaa tcatcttttt taataatttc atacttattt tctcttcaac atcatcaaat
    24901 gagggtgtct caaaattaac tttttacttc ataaggcact cttttttgta tgaagtaaaa
    24961 tatattttca tcatttcaac aataagtaag aggcttatct ctattttgag acagccccat
    25021 ttgatggtgt tgaacattta aataaggttg aactcgtttt atctctcttt ttaagatatc
    25081 tgtaaatttg cacataaagt ctcttcttct tttacaattc taatagtttt attaaagcca
    25141 aagtatatat ctccatattc tttcgtatat gatttattgg tactctttca tctactccat
    25201 gaccaccagt tttatacgct aagaacttag gtccaaacgc caccatatct ttaaaccaac
    25261 ttgcatatgt gccaccaccc atagcaattg tatatccatt ttcatctgtt acttcttcat
    25321 aggcttctaa taatttttta agtactaaat tactctcatc cacataatgt aagtctttat
    25381 gaacaataac ctctattttc caaccattac aaaaaacctt tttgaatcta tctattatat
    25441 tgtttaaaac tatattttta ccatacctta tatctaactc acatacgatt ttttcctcat
    25501 ttacttttaa tattcctaaa tttaaggtag tgtttttgaa tacctcatca tattgattaa
    25561 tccccaatgc atctccataa taatcactac aaaggtattt tgaaactaat tctatatctt
    25621 ctctatgctg aaaatccata ttatcctcaa ctatctttat taccatttta gatatagcat
    25681 tttctccctt ttcaggatta cttgcatgta cagctactcc tttagtagtt aactctgaaa
    25741 taattccatt aataattttt gcagtacaat gagatggaac aacattttct ttagttcctc
    25801 cacttactat tatgtaaggt ttacttttgt caattttaat ttctttttct atccttatat
    25861 gaacccttcc tttttctcca ttaaccactg gatacatagc atctggagta aatcccatac
    25921 ttggaatttc ttcaccttta tctaagtaat atttgataca tttcatacct gtctcctcat
    25981 ttgtaccaaa aattaatctt attcttttat taattgtagg atacatatcc tctatgtttt
    26041 tcaatacatg tataagcata gatacagcac ccttattatc tatgacacct cttccataaa
    26101 taatattgtc aattactgct cccgaaaatg ggtctacact ccacccctct cctggtggaa
    26161 ctatatctaa atgcattgga atccctatta attcctctcc ctctccaatt tctgcatatc
    26221 caatataatt atcaatattt ttaacactaa aacctaaact agcacagtaa tctaaagttt
    26281 tatctaaaga ttttttaggt cctataccaa aaggacattg tgaaataggt tcaccttgtt
    26341 gtgaatcaat ttttatcaat tcttttagaa attctatggt tctatttaaa ctattatcta
    26401 cacttctgct tatatccata aatcaaccta ctttctatct ttttaaagcc tgtccttaaa
    26461 gaacaggctt ttagcattaa aaattatttt tttgtaaata ctaattcaca aacttcatca
    26521 tcatgagcac atttttgagc aaaatctaat ttaacacctt gtgcacagtc aactcttcca
    26581 taatccccat aacaagctaa tctacaaatt tcttttcttt cttctttagt taatccatat
    26641 tctttccaag cagcatctaa tggacagtga tggaatttta aaacgccatt ctcatctgta
    26701 ttttctacta attccatttc aaacactttt tgtcctttac tgttatataa catttctgcc
    26761 atttttcctg gagtatctgc tacgttatat ttttgacctc tcattttacc aaatccatat
    26821 attgcttctt tacatatatc atctggatta gctcctaatt tttcagcttc tttcataaga
    26881 agatagaacc atactgctct ttctcctgtt gctcctctaa ttccttctaa atattccttt
    26941 tctgtataca ttttttctga catttctttc tcctcctaaa attaaattct atattttaat
    27001 cactgtgaat tagaatattc caatgaatga ccctaccaat gctaatacaa ccaaaattac
    27061 taataactta gttattgtcc acttcttgta tttcattccc caatatacga agaatacaac
    27121 accaagtggt aataaacctg gagcaatcat atttaatgta tcttgaacaa caattgcttt
    27181 accaccaata tcaaatgata gcggagttga aagcttaact gtagttgcag ataatgctcc
    27241 catcataaac attcctagaa ttgaagcacc atcaattatc ttattgacaa taccacctga
    27301 tagaattttc ttaattgaat ctctacctaa agaatatcct aaatgaaata agtaataacc
    27361 ttcgcaaaat agtaatatac taaacattat tgggaaaaat gcacctataa cacttccttc
    27421 tgctccaaat gaagcaccta aagctaagaa tattgtttga aatgtaccaa aatctaaagt
    27481 atctccaatt ccagccaaag gtcccataag accattctta acacctgaaa taacttctcc
    27541 tgggattttt ccttcagtag ctttctcttc ttccatagct aaaactgtac cgtgaattaa
    27601 tcctccccat atagcttgag tattaaaaaa ttgtaagtgt ctctttaacg catctactaa
    27661 atcttctttt ctcttatata acttttctaa agcaggagtg aaacttgcac aaactgctaa
    27721 tgcttgcatt ctttcaaaag agttggataa ttctgctgtc caccaccatc ttagatatgt
    27781 ttttgttaca tctttctttg taagtactct tgtcttatta acttcagttg gaccttgtcc
    27841 atttttaact tctttatttt ttatttcttg cattatactt cacctaccct tttgtcttct
    27901 ttcataaagg taattaaaag agctatacaa acaccaaata ttgcagctgc agtaattgat
    27961 atttcaaaat attgaaccat aaaaaatcct ataataaaca ttggtaagaa tttattttta
    28021 cctatagtaa atattgttgt tgcaaaacct aatgcaggta atataccgcc cattactgtt
    28081 aatccattca ttaaccattg tggtataaca tctaataatg attgaactaa atctgctcca
    28141 aaaaagttac atacaaaaac tattggaaac cttaaaacaa acccaaatat taaagaataa
    28201 agtgttgcac atctccagat gccttttgta ttaccttctt cagcatactt atctgcctta
    28261 tgaaccaata cagcattacc agttcttctt aagttattaa ctaatactcc tataacacct
    28321 aaaggtaccg caatagatac tgcaacttct gcattaacac cagtttgaag tgctactggt
    28381 attgcaataa gagcagctag acatttatct gatggaatat ttccccctgc tgcgaccatt
    28441 cctagataaa ccatttctat acttgcacca acaatcatac cagttgttac atcaccccaa
    28501 agtaatccaa ggctaaaacc tataaataaa ggagacttta gtgttgagaa aagggtatac
    28561 cctgcaagac ctgctgcaaa ccaataccat aatcctgtta caattgccaa catcaaaaca
    28621 gattccattt ataattcctc ctaatttata ttttttattt aaatttcttt aacgcatttt
    28681 catatgtcat agatgattcc tctggtataa cttgtaggac tatttcaacc ccattttcat
    28741 taagttcatt aagttgttct acatcctcat ttcctaatga aacagctgta aacaccattt
    28801 tctttcctgc ttcatttgga atacctccta attgaatctt ttcatattta agtccagctt
    28861 tataagcctt atttgccata tcaacatttt taaatagaat aaatatattt ttatctccta
    28921 aagtgttgtt ttggaatgct tgtcccgcat cttctgctgt taaaataact acttctactc
    28981 ctgatggtgc tgccatttta tatatgtcag ccataaattc atcttgacct aagaagtcat
    29041 caactattat tattttttga gcttgagcga tttttaccca ttttgttata acttgcccat
    29101 gaattaatct gtggtctact cttactaatg ttatttttgc cattatattc acctcataat
    29161 ttatattttt ttaaattctt taattaaatc cacacaaagg ttattatttt ttttgacgat
    29221 tctttctggt aatgtatgta atttatcttc tttgcttaat tcttcatcta cgattaaaat
    29281 tgtctgtaag ctaagtccat ttattgcatc tattcctctt cttatagcaa cagctattgc
    29341 aacacttgat gtagatcctc catataaatc tgatattata attttcacat cttctttatc
    29401 tactacttct tcaacctctt tcataaatac agaaacatct ttttcagaac ttagtgataa
    29461 aacttcatat tgatttgaca atccaaagtt tttatttgtt tcttctacta agacttttcc
    29521 ccaagttcca tggcacagta tgattacctt atatttattc attgctaaat aaactctcct
    29581 ttatagcgat taatattttg gtttaattct acttcttctt catcaggtac catttgaatt
    29641 gatacattaa tattcttttt aataaattca tctattaaaa gaaactcttt tttattaatg
    29701 tatacatttt gagatatttt cttttttcct attcctgtag gtattcttcc aacaataagt
    29761 tttttgatat taatcccttc ttctatcatt ctatttgcag tatctaaatt tttaattaat
    29821 acaaaaacgt tatcatcagc ttgattatta taaaaatatc tccttgattc acttacagtt
    29881 aatattgata aatcaatatt ctttggtgct gccattttta tcaaaccttt agcaataaca
    29941 tcattggttg tttcatcatc tactactact atctttttta cacctagatg cttaacccat
    30001 ttatatataa cttgaccatg tattaatcta tcatcacatc tgcaatattt tactaaagac
    30061 atttttaatc aaactccttt ttctattaca ttgagctaag aactttatta atattgttta
    30121 taccagtttg tcctgcttca atacatgctt ctgctaactc ttctaaactt tttccctctc
    30181 tattaattaa agcttcaagt agcattggaa gatttactcc agtaagtgca tgaaacttgc
    30241 cacctttttt aaggtttaaa cttgcaacat tacttggcga acctcctaat aggtcagcta
    30301 atactaaaac tccgtcacct tcatctaatt catttaactt ttctacaaaa tttttattta
    30361 agtcttcaat attatcattc ctatttagtt taattgctgc tgttctttct gctggtcctg
    30421 caataagttc acagcttttt ataatttctt cactaaaact cccatgagtc ataactatta
    30481 ttcctatcac caatttcacc tcacttttct tgtcactttt tatataagca attgccgtgc
    30541 caacttatgt gtatcaataa caaatggcta ttttacagga tttaatcaaa actttttact
    30601 caattttttt taaaaactta ttatttcgat atgtatcaaa agattttttc aacctgtatc
    30661 aaatctgtat caaaaaaagt aaaaaaaaga tagccttttg gctattttta aattttgtat
    30721 caacttttga atttatctgt atcatattgt gattcaaata tatctaatat atttgcatat
    30781 tccatatctg aaataattat atcgaatgtc tcttctacta gcttaaaact ttcttttata
    30841 actaaatata attctttatt tctactaatt ctttcttcat atgatttata aactaaagac
    30901 tcttttatta ttactctttc aatcatacat gaacaatgaa atataaactt tactaaaagt
    30961 tcatcttgaa ttactaaaga atagtgttca ctaattctat taattacttc ttttaatata
    31021 aaataagcct tttccccatt taaaaaagtc aaagttttac taatattatc aattagtaat
    31081 ctatcaaaat atctaccacc attatctttt tcagcatatg taacttccat atctccataa
    31141 tgtttcttta tagattctat aatattaaaa tataatttct ctaatgtcat ttctggagtt
    31201 agagcctttc tagttgcttc tattacaata ggtgtactta ccatatctat tgcttttact
    31261 tcttttcctg tagcatcacg aataacttta gcaaatgtta aaatagaccc catatcagtt
    31321 aaaattaaaa ttccctttcc tttatcaatt ttattggcta tttctacagc ttgtactaaa
    31381 atatcctgaa ctctatcttc taatcccata ttaattgcca ctgcatggtt acaatccagt
    31441 aaagtattgg ctacattcac catgctagat gctgtagatt caccatgcat tatgactaat
    31501 attgctatac tctcctcatt taatcccaca tacgataaat aaagaaatgt tgctaaaaaa
    31561 attgcttcat cttctggaaa ctctactcca tacattaatg acatgttaga taatatttct
    31621 tttgctatgt tatattcata tgctctttcc ttcataattt catctttatt aggatgcttt
    31681 actatatttc caatctttat tctttcaatt aaattctgaa tatgtaaagc tatagctttt
    31741 attaatttat ctaatccttc tcttttgctc catttatcat gcttttgtaa aatatatttt
    31801 acagtagttg caattgaatc attaacaatg ttgttataag ctgtttgatt actactatta
    31861 tatataaaag tattcattaa attataggaa tacttctgta tgtctccatc tataacactt
    31921 ctaatttgtg actctttaat accttcttgt tgaagtttat tccaagtttc ttttatcatt
    31981 ccataaaaat ctaaatcata ttttttattt tctattaata aattttcact ttcaaaatcc
    32041 ttttcttctg gtaaaaaaat aatatcatct tttccaataa aactgaaatt ttgtacaaac
    32101 tcctcttttc tattatttcc atataatgat tctctaatat tatttggtag aagagagagc
    32161 cttacatata cacattccct tttataggtc atataatcta aaaaagctct tgcacaaatt
    32221 aattgtatat cgctttttaa ttggcctatg tttcccttac acttatataa aataaatgcc
    32281 ttaataactt cttttgaaac ctttaactta acaccaatcc taacactctc ttctttaaaa
    32341 aaataacata taagcttcat tctctcttga aagcctcttt cctctaaaga cggaatcttt
    32401 atagttacag gtattcttct taaaaacgtt tccaacattg ctgtttctgg gtcttctgta
    32461 gtggctgcaa ttatcataac tctacactcg tgtaccttat tagcctctcc taaccttcta
    32521 tatatccctt tatccataag cagaaatagc atttcttgtc cctctgcaga taatctatga
    32581 acttcatcta aaaacaatac tccattatta gcttcatgta caataccata cttatcttta
    32641 tcagctccag taaatgctcc ttttacatat ccaaaaagct gcgctaataa taattgctga
    32701 ttatcagaat aatcagcaca gttaaatatg ataaatggtg cattatcact atcttctttt
    32761 atagttaaac tatacttata catatattct gcaaatatag atttaccaac tcctgtaggt
    32821 cctattatta atgtactcaa ccccttaggt ggatatatta ctgctgcttt agcctgttct
    32881 atttgggacc ttaaacttcc atcacatcct actaaattaa aaatattgtt ctcttttttt
    32941 atactctcta aaatatactc atctttgata gaatcatttt cactatattc gtttatagag
    33001 ttaatattat caaatatccc tttatttagt ttctttccta aaatactctc aatactttct
    33061 ttatataagt actgaacagg cttacccttt atctttataa taatactttc ttttattaat
    33121 tgatttaatt ccttactagc attacttcta tttataccta cttcagtagc tatatattct
    33181 gcatcaaatc caaatctaat gccattatta aagttcaagt gttcttctgt taaccttttc
    33241 aagcaaatta aaactttttc tttccttgat agcttctttt gtgtcatagt taacctcccc
    33301 tataagcagt aatactgtta aacatttatt attaagctta attctacaat attttaatat
    33361 ttttgtctaa ttatgtaact attgattttc tttcctattc gttttataat ataaattacg
    33421 ttaccctcat cttttataga ggaggaattt atatgaattt tttatcagat ttacacaccc
    33481 actctatagt cagtggtcat ggctatagca ctttactaga aaatattaat tattgtaaag
    33541 aaaatggaat taagatacta ggtacatctg aacatggtcc taagatgcct ggttctcccc
    33601 acagatggta ctttcacaac attcaaaata tcccaagaat cataaataac attataattt
    33661 taagaggctg tgaagcaaat atactagata ttaaaggaaa catagaccta gagccttttg
    33721 taattcctcg cttagattat cttattttgt catttcacga agctgtgttt tctccaaata
    33781 cattagagaa taatactaaa gctttaataa atgcaataaa taagcatgat aatattgaaa
    33841 tattaggaca ccttggaaac ccaaactacc caatcgacta tgaattaatc attaaattag
    33901 ctatagaaaa gaatatctta atagaaataa ataattgttc tattaaaggc gtgtcaagaa
    33961 atggtagctc ggataactgt aagtatattg ctacactttg caaaaaatat ggtgctaaaa
    34021 tcatactgac ttcagatgct cacatttgtt ttgacatagg taactatgag tattctgaaa
    34081 atatcttaaa ggaaattaat tttccagatg aattaataat gaattatcct aaaaaactta
    34141 ttaatcattt tcaccaaaaa ggtaaattac tagatgttga ttatacaaat atctaagtaa
    34201 taatacatgc ttactttaac tcccataaaa attttaatac tatttatatt atatgaaata
    34261 aagaaattat aataaagaag ctatttaata aagcttaatt acttttatga acttgatgga
    34321 ttgatatggt ctatgatagc aatagaaatt ataacatttt taataaactt agttttatat
    34381 accttatagt ataaatctaa aaaagcatca tctaaaaagg agctgtcaca tgagcaaatt
    34441 aaatttgttc tatgatagct ctttttttac ttattttaat taggtatatt tttaattatt
    34501 ttatataaag cttatgttct aagtaaaatt atgcataaca aacaaaggta taaaaatacc
    34561 attttttcag ataatattta attataaata ataaatttag aatacttctt ttaaatgatg
    34621 agagtctaga aatttttatg tgtattcttt aaataaaaat ttccagactc tagccccttt
    34681 ttgaatattt taaaatttaa cttaaactaa gcaaattcta taatagctta tctctaaata
//
LOCUS       DAMJWS010000038        34657 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMJWS010000038 DAMJWS010000000
VERSION     DAMJWS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.038"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(68..724)
                     /locus_tag="PC399_001947"
     CDS             complement(68..724)
                     /locus_tag="PC399_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2592807.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(779..1408)
                     /locus_tag="PC399_001948"
     CDS             complement(779..1408)
                     /locus_tag="PC399_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2592808.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(1539..2180)
                     /locus_tag="PC399_001949"
     CDS             complement(1539..2180)
                     /locus_tag="PC399_001949"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2592809.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(2189..2860)
                     /locus_tag="PC399_001950"
     CDS             complement(2189..2860)
                     /locus_tag="PC399_001950"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2592810.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLSDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(2865..3749)
                     /gene="rsgA"
                     /locus_tag="PC399_001951"
     CDS             complement(2865..3749)
                     /gene="rsgA"
                     /locus_tag="PC399_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2592811.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(3847..4584)
                     /locus_tag="PC399_001952"
     CDS             complement(3847..4584)
                     /locus_tag="PC399_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2592812.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(4692..6707)
                     /gene="pknB"
                     /locus_tag="PC399_001953"
     CDS             complement(4692..6707)
                     /gene="pknB"
                     /locus_tag="PC399_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2592813.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(6724..7476)
                     /locus_tag="PC399_001954"
     CDS             complement(6724..7476)
                     /locus_tag="PC399_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2592814.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(7496..8527)
                     /gene="rlmN"
                     /locus_tag="PC399_001955"
     CDS             complement(7496..8527)
                     /gene="rlmN"
                     /locus_tag="PC399_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2592815.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(8535..9860)
                     /gene="rsmB"
                     /locus_tag="PC399_001956"
     CDS             complement(8535..9860)
                     /gene="rsmB"
                     /locus_tag="PC399_001956"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2592816.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(9922..10623)
                     /locus_tag="PC399_001957"
     CDS             complement(9922..10623)
                     /locus_tag="PC399_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2592817.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(10692..11444)
                     /locus_tag="PC399_001958"
     CDS             complement(10692..11444)
                     /locus_tag="PC399_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2592818.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTVVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            complement(11454..12383)
                     /locus_tag="PC399_001959"
     CDS             complement(11454..12383)
                     /locus_tag="PC399_001959"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2592819.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(12398..12838)
                     /gene="def"
                     /locus_tag="PC399_001960"
     CDS             complement(12398..12838)
                     /gene="def"
                     /locus_tag="PC399_001960"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2592820.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(12856..15381)
                     /gene="priA"
                     /locus_tag="PC399_001961"
     CDS             complement(12856..15381)
                     /gene="priA"
                     /locus_tag="PC399_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2592821.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(15620..16819)
                     /gene="coaBC"
                     /locus_tag="PC399_001962"
     CDS             complement(15620..16819)
                     /gene="coaBC"
                     /locus_tag="PC399_001962"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2592822.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(16824..17090)
                     /locus_tag="PC399_001963"
     CDS             complement(16824..17090)
                     /locus_tag="PC399_001963"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2592823.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(17093..17710)
                     /gene="gmk"
                     /locus_tag="PC399_001964"
     CDS             complement(17093..17710)
                     /gene="gmk"
                     /locus_tag="PC399_001964"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2592824.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(17740..18621)
                     /locus_tag="PC399_001965"
     CDS             complement(17740..18621)
                     /locus_tag="PC399_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2592825.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(18625..19455)
                     /locus_tag="PC399_001966"
     CDS             complement(18625..19455)
                     /locus_tag="PC399_001966"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2592826.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(19742..20776)
                     /gene="ltaE"
                     /locus_tag="PC399_001967"
     CDS             complement(19742..20776)
                     /gene="ltaE"
                     /locus_tag="PC399_001967"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2592827.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            21072..22847
                     /locus_tag="PC399_001968"
     CDS             21072..22847
                     /locus_tag="PC399_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2592828.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(23062..24714)
                     /locus_tag="PC399_001969"
     CDS             complement(23062..24714)
                     /locus_tag="PC399_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2592829.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(25013..26224)
                     /locus_tag="PC399_001970"
     CDS             complement(25013..26224)
                     /locus_tag="PC399_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2592830.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(26508..27041)
                     /gene="pyrR"
                     /locus_tag="PC399_001971"
     CDS             complement(26508..27041)
                     /gene="pyrR"
                     /locus_tag="PC399_001971"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2592831.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(27401..28348)
                     /locus_tag="PC399_001972"
     CDS             complement(27401..28348)
                     /locus_tag="PC399_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2592832.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(28317..28763)
                     /gene="lspA"
                     /locus_tag="PC399_001973"
     CDS             complement(28317..28763)
                     /gene="lspA"
                     /locus_tag="PC399_001973"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2592833.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(29073..29852)
                     /locus_tag="PC399_001974"
     CDS             complement(29073..29852)
                     /locus_tag="PC399_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2592834.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(29907..30389)
                     /locus_tag="PC399_001975"
     CDS             complement(29907..30389)
                     /locus_tag="PC399_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2592835.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(30655..32076)
                     /locus_tag="PC399_001976"
     CDS             complement(30655..32076)
                     /locus_tag="PC399_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2592836.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(32222..32992)
                     /locus_tag="PC399_001977"
     CDS             complement(32222..32992)
                     /locus_tag="PC399_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2592837.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(32997..34199)
                     /locus_tag="PC399_001978"
     CDS             complement(32997..34199)
                     /locus_tag="PC399_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2592838.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        11029 a         6362 c         3478 g        13788 t
ORIGIN      
        1 tttttaaata tttattttta atttattttg tttttaattt acttcgattt taaaatgtcc
       61 ttgactttta ttttataaat gtacatattt cttctactgg ttttttagat ggactcttca
      121 aattgtcctc tggcactcca agagctaaca ttgcacctaa aaaatactct cccttttcaa
      181 aaccagtttc tgcttctaaa acagcttcta tctcatctgc tgcatagttt tgacttgtca
      241 accaacaaga accatatcca agttcaattg ctgatagtgt aaagttttca attgcagcac
      301 ctagactctg catacctgga tttcttataa ataatttatc tatagtctcc ttaggtgcat
      361 ctataagctc taattcataa tatcctgatg gattatatac ctttgtaaaa actaatacta
      421 aaactggagc ttttaagtaa aatagtgtaa aatttttaac aaattttcta aatctattag
      481 ccttatccac tgatacttta tccatttcta caagtatttc ttgttgcttt ttagttatca
      541 catctgctat cttttccatt aaatcacggc gttttataac aacaaaatgc cagttttgaa
      601 tattcttgcc tgatggtgct gcacctgctg cttttatcat ttttaaaata tcttcatctg
      661 atacatcttg atttttaaac tttcttacac tttgtctttt aaaaatagta tcttgtaatt
      721 ccatttttaa atccccccat aactttttta ttattttaat ataattttta aactcatctt
      781 attttaatat atcatgttct aacaaatctt taaatgaatc tataaaataa tcatatacat
      841 ctacattccc aaacccatat ttggcatata taaaaggtat acctacaaac ttagcactat
      901 ttgcatcacc ttgcgtatca ccaacataga ttggatttat taatttattt ctttcaatta
      961 taagtttatt attttctcct ttatgcaaac cagtattacc aggacattca aaatctataa
     1021 aatactgttc tagtttatgt gcttttaaaa aagattctat atatccagct tgacaattac
     1081 ttactataaa taatttatat tttttagata gtaatttaag agtatcttct atatcatcaa
     1141 ataatttagc tccattttca gataaatagt cacattcttt caaagaacat tcattcaata
     1201 aattcattct ttcttcttca cttaaatttg gaaataaacg atttgctatt tcctcaaaag
     1261 aaagccccat aagacttcta aataaatctt ttgtgacact taattctatt tccttatgct
     1321 cacttaaaac atcctgccag actttgcata cttcttcagt tgaatcccac aatgttccat
     1381 ctaaatcaaa aataatacta tctaccacaa aaaatccccc ttatgtcaaa gtttattata
     1441 tatttataat tatatactaa aaagcagata caatcataaa tatattttac ttgattgtat
     1501 ctgctctaca atataaaacc aattgtcaaa atcaaatttt ataaatttct aactacaagt
     1561 acacagcctt gctctacttt aatcttacaa gaattactca acataacatt tgatattcca
     1621 ataggtacag agtatttcat ataataatta tcgagagggt attctaagcc agtaagtgtt
     1681 acacccttag catcaccatt tatagctatt acagaaatag tgtcccctgg gtttccatac
     1741 aatgaaattt cctcattttc aactatatac atctcttctt tatcagaaag tatcctagaa
     1801 tatataccat cttcttttaa ataatataat agctttatat ttgcaagagt gtggtcaatc
     1861 cttcccccta aagcaccaaa aaaatcaata tgatttgctt taagtgtctt agctaaaaaa
     1921 agacaaagct ctgtatcagt ctcatctttt ttagatggaa atttttcaaa tttaacacct
     1981 ttatttttat agaagtttat cttttcttct tctactgaat ctaaatctcc taatatataa
     2041 tctggcatta tttccatttt ataagtgtga tttgcccctc catctgcaca tattatgcag
     2101 tcataacatt ccttaattat aatatctctt gtaacttcgt aatcttcaat ttcgccattt
     2161 aaaacaatac agattttcat tattaaattt aaactaattc ttcaagagaa gcattctttc
     2221 taaataaatt aactgtttct tgaatgtcat cacttccaaa tattgcagaa cctgctacta
     2281 ttacattagc tcctgcttgt acaacttcgg ctacgttttt agggcttata ccaccatcaa
     2341 cttctatatc aatgtttaat ccttgactat ctataatagc ttttaactct ttaatcttat
     2401 ctaaaacaca aggaataaat gattgtcctc caaatccagg gttaacagac attaataata
     2461 ccatatctaa atcagatagt acatgtttaa tcgtatctac tggtgtagct ggattaagta
     2521 ctacaccagc ttttattcca tgtgatttta tattttgtat agttctatgt agatgaacac
     2581 aagcctcctg gtgaacagtt attatatcac atcctgcttt tacaaattct tcaatatact
     2641 ggtctggatt ttctatcata agatgagcat caaataccat atttatattt tctttcttta
     2701 aacttttgac tacaagtggt cctaatgtaa tgtttggtac aaagtgtcca tccattacat
     2761 cgatatgaag atattcacat cctgcacttt ctacttttct tacatcttct aataattttg
     2821 caaaatctgc tgataatatt gatggtgcta atttaatcat gtttctaata cctcctacta
     2881 ttattttgtc ttatttcatg tagcagctgt atataactat catatctttc tttagatatt
     2941 tctccatttg taacagcttc ttttatagca caattaggtt cattttcatg aatacacttt
     3001 gaaccaaact tacaatcatt aaatttatca aattctataa aataatcttt aagttcaact
     3061 tcctctatat cttcaagtgc aagggaacta aaacctggtg tatctgcaac cattccacca
     3121 aattctaatt ttaaaagttc agcgtgacga gtagtatgtt ttcccctttt tattttatca
     3181 cttacaactc ctgtttgaag tttaaaattt tcatctattt catttaataa gcttgatttt
     3241 ccaactccag atggtcctgc aaacactact acattttctt ttaattcttc ttttacttta
     3301 tctatattta acttagtaat attgcttaca ggaatgactt tataaccact aagttcatat
     3361 atatttttaa cagtttctaa tgtatcatta tcatctaaat ctgcttttgt aaggattatt
     3421 acagtttcta aattttcttt ttctgccaaa actataaatc tatctaaaag agataagttt
     3481 ggtttaggat tttttattgc gaatactatt aatgctttat ctacatttgc aataggaggt
     3541 cttataagtt ctgtatctct actgtcgatt tcttctaaga tacccttttt ttcatcttca
     3601 tcaacaatgc ttattttgac tctatcacca accaaaggtg ttattttctg ctttctaaat
     3661 atccctctag ccttacattc ataaatacca ttataagtat ctacataata aaatccacta
     3721 atccccttta taatttttcc ttctagcatc aaatgcctcc ctttataaaa tttatataaa
     3781 cacatactgt ttatgagtag tatatcgtgt tattctttcc tatttttata taataccaca
     3841 ttttttttaa ataacaaatc ctccgtttgg actaattacc tgaccagtga taaaatttga
     3901 tttgtctgat gccaagaaaa tagcacaatt tgctatatct tctggagttc ctagcctcat
     3961 tagaggggtt tcttccacaa gagcgtctat atcttcctca ttatatccag ataacatgtc
     4021 agtattaatg acacctggag ctatgctatt tactctaata tttgatggtc ctacttcttt
     4081 agctaatgcc tttgtcatac ctattatacc tgcttttgtt atagaataat gcacttcaca
     4141 agacgcacct gatatacccc agattgaaga tatattaatt atatttcctt ttttttctga
     4201 tatcatgtat tttaaagcaa cttgtgaaca ataaaagctt ccttttaaat taatattcat
     4261 catattatcc caatcttcat cagttatatc tgtaaacaac ttatcttgac ttacaccagc
     4321 attgtttaca agtacatcca atcctccaaa ttcttttata caataatcta ccatatcctc
     4381 tacatcttct ctatttgaaa tattagcctt aaaaagtttt acagaaaaat ttttttcatt
     4441 taatatagtg tacaactctt tagcttcatt ttctgactta ttaaaattta ctaatacatt
     4501 gtaaccttct tttgcaaaag ctttagacat agctttacca atccctctag ctcctcctgt
     4561 aattaaaact gttttcttct tcataaatac ttttctcctt atattataac caattcagta
     4621 ttttatacaa aacaaactgc cctttaaaga aaatattttc tataaaagga cagctcttat
     4681 tttattaata tttattctgt tttaccaatg tttgcgccag ttcctgatga agttcctcca
     4741 ttatctcctg agtttgagcc tcctgaacta tctccattgt ctcctgagtt tgaacctcct
     4801 gaacttcctc cattgtctcc tgagtttgag cctcctgaac ttcctccatt atctcctgag
     4861 tttgagcctc ctgagtttcc accactattt cctgagtttg aacctcctga gttacttcca
     4921 ttattgcctg agtttccacc actatttcct gaatttgagc ctcctgaatt tccaccactg
     4981 tttcctgagt ttgaaccagg ttctgttggt gtattattct ctggtgttgt tggtttttta
     5041 tctggtactg ttggagtctg tgtatttgat ttttctgaac ctttgcttac atatagattt
     5101 acctcatcac cttcctgagc tcttgatgag ccagcacctg gactctgact taaaactgta
     5161 ccttctttat atattgagct gtattcatac ttaacagttc ccacttttaa gttattttct
     5221 tcaataagtt gttttgcatt actcaaagta agtcccacta cattaggaac agatgcctga
     5281 cttgaacctt tacttacgac aacatttata gtatcaccct ttttgacatt tgtacttcca
     5341 ccttcaggtg tctgtgatat tatacaattt tcatcaactt cactattata ctcttcactt
     5401 tttacactca ggtataatcc ttctttttca acagcacttt gagcttcttc taatgtcata
     5461 tttacaagat taggaacagt caaagattta ttgccaaacc ctccagcaaa taaaaactta
     5521 tatgctaaga atacttgagc acacaatatc agtatcaaaa cagccgcaat tacttttaat
     5581 cttcttctag attttgggct ttcctgtttt ttcttctttt tcttagttct cttacttgga
     5641 gtactcttag gtctctgatt ctttttagct ctcattatct cttcctcttc ttcatcttca
     5701 tcatcatctt cttcataaaa atcatcatag taatcttcat catcatccaa atcagctact
     5761 tcaacaggct taaccacttt ttcaggagct ggttttgcaa gtgttggatt tacaacttta
     5821 ttaatttctt tttcatcaat tttcttagtt gcaaaatcat catattcttt tataaaatct
     5881 aaatcaatat ttttttctat atactctata tcttctatca attcttcagc agtttgatat
     5941 ctatctgcac tagatttttc tgtaagcttt tttattgtag ttcttacact ttgaggaatc
     6001 ctaacttttt cttctgatgt aaagtctata tcatcattaa tgtgttgaag tgctatagaa
     6061 attggactat ctcccctaaa tggaactttt cctatcaaca tttcatataa aactatacct
     6121 agagaataca ggtcagcatt attagttaca aatttacctt tagcttgctc tggtgaaaaa
     6181 taatgcactg aacctattat actaccaata ttagtcattg ttgaatttga aacagctttg
     6241 gctataccaa aatcagccac ttttactact cttccttcat ttgatatgag aatattatga
     6301 ggttttatat ctctatgtat aatccccttt ttgtgcgctg cactaagtgc catagctatt
     6361 tgttttgtta tatctaatgc agtatattca tctaatatac cttcatcttg tataatctct
     6421 tttagattct tcccatctac aaactccatt acaatgtagt gaacttttcc atcttcacca
     6481 acatcataaa catttactat atttggatgt gaaagacttg ctactgcttc tgcctctctt
     6541 ttaaactttg ttagaaattc atcatcatct acaaattcag gtctaagtac tttaagtgca
     6601 acagtcctgt ttaataatct gtctttagcc tcatatacaa atgccattcc tccatcacca
     6661 atcttcctga tgatttcata tcgatttcct aaaattgtat ctcccacaat atcaccgtcc
     6721 taattatact tttatcacta taaccgaaac attatcttta ccagaaacat cattggccat
     6781 acttatcaat tcttcactta tattactagt tctctcatat tcataatcta atattaaatc
     6841 tcttatatct tcatcatcaa taaacccagt taatccatct gtcgcaagaa gtattatatc
     6901 actttttttg atttctttct taaaaatatc tacaacaacc atatcatctg ttccaattgc
     6961 tctagttatt ctattcctct gtggatgtct tcttgcctct tcttctgtaa tgacatgggc
     7021 catcataagt tcttcaacca cggaatggtc tatagttatt ttatcaaatt tttcatctgt
     7081 taacaaataa catctgctat ctccaacatt tgccacatat aagacattat tatacactat
     7141 agccgtaact aatgtagttc ccattccttc aaattctata tcttccatag attttttgtg
     7201 cacaattgag tttacattat tataagcctg ttttaatata tcatctatat agtctatttt
     7261 tacattatca tgttgtaata agttttcctt tagaaaatca attatatttt ctactgctaa
     7321 tttactggcg acttcacctt ttttatgtcc acccattcca tcagctatgg caaatattcc
     7381 tattggacca tgttctgtat ctataacttc tccctcacaa tagtcttcat tgttttttct
     7441 tatttttcct atatgggagg cacaactata aaccatacga cttcctcctt acaaattact
     7501 tgtattttct tctcaattgc ccacaagctc cactaatatc agaacccata gacatcctaa
     7561 ctgtggcagg tatattgttc ttctccaaac tatccctaaa tttataaata aatgctttat
     7621 ctggcttttc atattctctt tcttcaactt tatttattgg aattaaattt acatgacata
     7681 gcataccttt taggagctta gctaatgcct ttgcttcttt ttctgagtca tttacccctt
     7741 ttataagaga atattcaaat gtaacccttc tatttgtctt ttttatataa tatctacatg
     7801 cattcaatat ttcttcaata gaataagcat ttgctacagg cataattttt cttcgttctt
     7861 catcatatgg tgaatgaagt gataaagcta aatttatagc tatttctaag tctgccaact
     7921 catacatttt aggtactatt ccacaagttg aaagagttat atgcctatac cctatattaa
     7981 gaccattttt ttcgttaatt atttttaaaa attgttttgt attttcaaag ttatcaagtg
     8041 gctcaccact ccccatcaat acaagatttg ataccctctt accagtatct tcttgaattt
     8101 taataacttg gtctaaaatt tcccatggct ctaagtttct aattaatcca tccattgtag
     8161 atgcacaaaa attacatccc atcctacaac ctacttggtt agaaacacag acagtaactc
     8221 tactatcata atccatcatc acagtttcta ttatatttcc atcatctaat aaaaacaaat
     8281 atttttttgt attatctacc ttagattcta actttaattc tatatctata tgccctatac
     8341 atgaaacctc ttctaattta tttctcaaac tttttggtat attgttcata tcatcaaaag
     8401 tttttgcacc cttatatatc caagagaata tttgacttcc tctaaagggc ttttcatcta
     8461 tagttttcat aaattcttta agttcatctt cagtaaaatt ttttaatact atctttttct
     8521 tttccattct ctcactacct tattctcttt aattttgcta tgaaaaaacc atctatacca
     8581 tgaatattag gatatatctt taaatatcct ttatcttgat tgtctaagtc tacatttact
     8641 gtatcaattg gaacaaattc aaagttgtga ttttcttcaa caaaagaagt aactatattt
     8701 atattctcca catcttgaat tgtacatgta ctatatacta aagttccacc aactttgaca
     8761 tatttagatg cattttctaa aattttcttt tgtatagaag tgatgtcttt taactcttct
     8821 tctttcttat acttaatttc tggtttacgt cttattatac caaaaccaga gcaaggaaca
     8881 tctgctaaaa cataatcaaa tttacttata ctattttcat caatctcaga agcatcaaat
     8941 ctttctacaa acacattttt aagccccaat ctatttacag tagagtttat aagctttagt
     9001 ttatgctcaa aaatatctct agctactact tgaccagtat tgttcattaa agtagctaag
     9061 tgagtagatt tccctccagg agcactacat acatccaaaa caaatgagcc ttcttttgga
     9121 ttaattactt tccctactat catagaactt atatcttgaa ttgtaaataa accagcctta
     9181 aataattcat ttttttctat atttttcatt ttttcgactt taatagcttc ttctaccata
     9241 ggaactttta aacatacaat acccatttca cttaattttt caataagttc ttctctactt
     9301 attttaagtg tattcgttct tatatagata ctaggctttt cattatttgc ttccaacaaa
     9361 tcttcagcaa attcttgtcc aaatttatct atccagtttt ttataatcca agagttatag
     9421 gaatacttta tagataagta ttttacacta tcttcctcag ttatgtccat tatagtctct
     9481 ttagctctta tttcatttct aagaatagca tttatgaaac cagaagattt tttatcatat
     9541 ttcttagata attttacagt ttcattaact gctgcataat ctgatatact atctaaaaat
     9601 aaaatctgat atgttcccat tcttaaaaag attttaacat aagttgacat tttttttacc
     9661 tttatttttg aaagtttatt gattatataa tctaaataat atttattttc tattactcca
     9721 taaattaact ctgttgcaag tcctctatcc atatcactta tttctaaatt tttaaaatgc
     9781 ttatttattg caatatttga ataattgtca tttttttcta tatcacataa tactttaaat
     9841 ccaacttctc ttgcatccat ataaatttct cccttttatt aaacataaaa aagggttgtt
     9901 tctaaacaac ccactttact tttagtcatt tcttctttga gctattagta acaatctaag
     9961 taattgaagt attgaaacca atgctgctgc aacatatgtt aaagctgctg ctgaaagtac
    10021 tcttctactt tgtcttgact cactttcatc tataatacct aaattcccaa gttgaacgat
    10081 tgctctactt gaagcattaa attctacagg caatgttatt atttggaata acactgaagc
    10141 cgaaaacaat agtataccaa tttttaaaaa tggaccagcc ataataaatc ctaaaaatat
    10201 taaaaaccaa gatatacttg aagcaaaatt cactactggt actaaactac ttcttatttg
    10261 aagaggtgca taacctttag catgttgtat ggcatgacca cattcatgtg cagctactgc
    10321 aacagaagtt atagatgttc cataatatac atcttctgac aaccttacag cctttcttct
    10381 tgggtcataa tggtcactta gatgccctct aaccatttct atctttacat catacaaacc
    10441 atttgaatca agtactcttc ttgctgtttg ctctccagta tatcctcgac gtgtatttac
    10501 tcttaaatac ttatttgttg ttgaactcac tttaaattgt gcatatatag taagtaatat
    10561 agctggaatt aatacaacca ttgttgggtc aaatccccaa aaaccgccat atacagggta
    10621 cataaataac actctcctaa aatatatttt tattatacat cctcttaata ttcacttaac
    10681 ttaaaaacag tttaattaca aactaatata ttttttcctg ttaaaaagcc tatagctttt
    10741 tcaatatcca tcatgtctac attagtattt acacaaggtc cattaggtct tttgttaaat
    10801 acacctaaaa caggaatttt cttaatatct tgtattccag atgtcaaatc tctttcacat
    10861 gcaacagcta caacagcttt aggtttagtg tccattataa tttttctagc tagagttcct
    10921 cctgtagcta caaatatttt tacattagtt ttttccttta gcttaattaa ttctcctatg
    10981 ttgcatcttc cacattcttt acaattatct atatcattag taacttttaa tttacaacta
    11041 ttctcttgaa tacaatgagg tattaaaatt attatatcct ctggattaaa attatattta
    11101 ttactgtata tatattcatt atttaacttt atatatattc ttctaatatc actttttgac
    11161 ataccaaatg atgatgcaat gaaagatata ataggaaata atccactgac aatattaaaa
    11221 tttattttca ttatatttga gttaacatac tttcctttaa ttactctata agtaactact
    11281 gttgaacata gtattataag tattagtgat attattataa tatttatcat caaggagaat
    11341 atttgagtta aacttattat aaaaaaatta acaactccta tcaaaattcc taatattaaa
    11401 atcaaaatcc caacacttgt taaatacttc tttatatctg tcatactttt cacctaaccc
    11461 aatactaaac cagtttctat agtattccct tttatatatt caccaaccaa cattctcttt
    11521 ttattcggca tctgaatttc ttcaattaaa agtacattat ctatagtact tacttttatc
    11581 ccatctttgc ttacatcaat tattgtacct acatcttttg tgcttttttc atctaacact
    11641 ttagttttcc aaactttcat tatgacatca ttataggtag tataagcact tggccatgga
    11701 tttactcctc taactaaatt gtgtatttct ctggcacttt ttgaaaaatc tatgttacct
    11761 aatgatttat tcattattgg cgcataggaa aattcttcat gattttgtac ttctcttgga
    11821 gcatttcctt cttcaattaa tcttaatgtt tcttttaaag tttctgctcc tatattcatc
    11881 attttatcat gaagttctcc tgctgttata ttttcatcaa gatttacttc tgtctttagt
    11941 atcatatctc cagtatctaa tccttcatcc atatacatag ttgtaacacc agtcttttct
    12001 tcaccattta ttattaccca atttataggt gccgcacctc tatattttgg aagtaaagaa
    12061 acatgaacat ttatacatcc aagtttagga atctctaata tttcttttgg aagtatctgt
    12121 ccaaaagcta caactactat tacatctgga tttaaagatt taattttatc tataaattct
    12181 ttatctctag cctttactgg ttgatataca ggtatattat tttcaattgc cagttctttt
    12241 actggactca tacctagttt tttacctctt ccttttggtt tatctggttg agttacaaca
    12301 cccaatattt catacttctc atctattatt ttttgtaaac atggaactgc tatatcagga
    12361 gttcccataa atactatttt catcaaatca actcctacta cttttctatt ttgtcaacaa
    12421 ataaaattcc atctaagtgg tctatctcat gacaaaatgc tctagctaaa agttcttctc
    12481 cctctaactc tattgtttca ccatttctat ttaaagctct gacctttaca taatttggtc
    12541 ttctaacatt tcccgcttct ccaacaacac ttaaacatcc ttcctcatct atttgctccc
    12601 cagatgtttc tattatttca ggatttataa gttctataag ctcttctcca atatctatta
    12661 ctactactct ttttaatatt ccaacttgag gtgcagccag tccaacacca tctgcatcat
    12721 acatagtttc tgccatatca tctaataatt gtattatctt ttcatcaatt ttttcaacct
    12781 tttttgattt ttttcttaat actggttctc ctatttgaac tatttgtctt aaagccatta
    12841 taattcctcc taaatttata atacactgtt tggattaata tctatagata cattgatatt
    12901 cttatcaaat atcaaatctc gttttgttat acatatatat tttattatac cctttaagag
    12961 attaatttca atattttcat ctttaaagag tatttgccat ctataatttt gatttatttt
    13021 tgatattgaa catggatttg gacctaatat aaatcctaaa tcatttatac cccttttttc
    13081 taataaataa atcaatgatg catgcatatt ttttatattt tttatcacta atctttcatc
    13141 ttcaccacta acaactacac ttaacatatt attaaaagga gaatatccaa atgcttttct
    13201 tatttttatc tcatcttcat aaaaaccctc ataatcatat tcaatagcat gcttaatagc
    13261 atagtgttca gtgtcataag tctgaagtac tacttttccc tctttatcag ctctccctgc
    13321 tcttccagag acttgagtta tcaattgaaa tgtagtttca aaacttttaa agtctggaaa
    13381 atttaatatc atgtctgcag acaaaatacc tactaaagtt acattctcaa aatccagccc
    13441 tttacttaac atctgtgttc caataagtac atctgcttct ttatctttaa atttatttag
    13501 tatctcatct aaagcacctt tttttgaagt tgtatcttta tccattctca aagtttttat
    13561 atctggaaaa atatatttta attcctcttc aattttttga gttccaactc caaatggctt
    13621 aacataagta cttttacatt caggacactc ttttggaatt tctttctcat atccacaata
    13681 gtggcatctt ccagtattgc ttttcttatg ataagttaaa gatatatcac aattttcaca
    13741 ttgaaacaca tatccacact ttctacaaga tacaaaattt gcataacctc ttctgtttaa
    13801 aaatagtatt acttgattat tttcttcaat cgcataacgt atttcttttt gtaatttcaa
    13861 actaaatata cttctatttc ctttatctaa ctcatctttc atgtctacaa cttcaatgtt
    13921 tggaagaggc ttatcatttg ctctactttt tatatttata agtttgtatt ctccagattt
    13981 tgccctataa tactcttcaa ttgagggcgt tgctgaacct aaaataagag ttatattatt
    14041 ttttaatgcc ataaatctag caacttctat agcattaaat tttggattct tctcagactt
    14101 atatgatgac tcatgacatt catcaattat tattaagcct aaggaattaa aaggtgcaaa
    14161 taaagctgac ctagctccaa ttaacactct tactttacct gctttgacag ctttgtaaac
    14221 gtcatgtttt tctccttcag atagtttact gtggaacact ccaactaaat ctccaaacct
    14281 actctttagc ctatctattg tttgaggagt taatgctatc tcaggtacta agaatatact
    14341 gtctaagcct tgatttagag caaattctat tatttccatg taaacttcag tttttccact
    14401 tccagttact ccatgaatta agtatggttt tttatcatct acaaacatat tcgactttat
    14461 ctcatcaact gcttcttgtt gctcattgtt taattttata ctttttagta cggatttgta
    14521 aatacttttt ggctctctat aataatcctt aaattctagg gttattagct ttttcttact
    14581 tagagaattt atactttgtt ttgaagcatt taataaatcc aataaatcat ttatctctac
    14641 attttcatta tttttaagaa aacttaatat ctctttttgt ttacttccca catttatttt
    14701 attttgctct atataatcgt caatcttatc actttctaaa ctcaaagata tataacatac
    14761 tttcttttca tttttgtgat ttttatattc ccaacataat tttataattc catttttatt
    14821 cattgtgtac aaagaactat ttaagtttgg tatatatttt aacttatcaa ccttaatttt
    14881 acctttacta tcaatcactt tctctactat ttctctattt ttatcactta aagttgatat
    14941 tttttccttc aattcaatat catttaatcc acttaaactt tcacctaata ggactacttt
    15001 ataattattt aatttgtatc cctttggata tatcaaattt atacattcta tataagtaca
    15061 caaatatcta tttttcatcc aataaaccaa ctctaaatct tcttttctaa atatcggatt
    15121 ttcatctaat atatcaataa tttcttttat ttttattttt tcatctaagc tatctgtaaa
    15181 ttgaaataca aaggcttctg ttggtttatt accttttcca aatggtacta atattctgtg
    15241 cccaattttt attacatcag ataaaaatac aggaatttga tatgtaaata aattatctgt
    15301 atatatagta ttacttctaa ctattacctt tgcatatttt ttcattataa aacctctact
    15361 taaatttaat atattgttca tattttaata tactttacaa gatattaaaa tctcagaatt
    15421 tataagcaat ttcaagttat taatattaac tttattttaa taattaacta ttaattatat
    15481 tttatacttt atgacaaaat aaatgctgaa aactacaatt taactgacat ttgcgagttt
    15541 ttactcgcta aaattaaatt gcagctttca gccactttag ctgtgataaa atacacaaat
    15601 atcatattta aaaaaggtct tatttctcaa gcaacgcttt aactttatct aaaataacat
    15661 tggctacttc ttcttttttc atcttaggat actcagtaat atttccctct acatctatta
    15721 tttttacaat attagtatca gctctaaaac cagcaccttc tttagttaaa tcatttgcca
    15781 caataaaatc taaatttttc ttttttattt ttcctttggc atgttctata aggtcattag
    15841 tttcagctgc aaatccaatt agtatcttat cttcttttat ttttcctatt tcataagcaa
    15901 tatcataatt tctacttagt tctattgata aatcatcatt cttcttttta atttttttgt
    15961 cagaataagt ttttggttta taatctgcaa ctgctgcact ttgaattact attttatttt
    16021 cacttaaatt tgatactact gcttcataca aatcttgtgc tgactctatt tgaacaaatt
    16081 ttttaagatt ttgtggaata gaaagatttg taggacctga tattaatgtt acatcagcac
    16141 ctctagctat agcttctctt gctatagaat atcccatttt accagaagac ctattagtta
    16201 aatatcttac tgggtctata ctctctacag ttggaccagc tgtaactatc atactttgac
    16261 cttttaaatc ttgctcttta gtaagattag tcaaaacttc ctctactatt atatctggat
    16321 tagctagttt acctgttcca atatctccac atgcaagcct tccactttcc ggttcaataa
    16381 atttatatcc aaagctcttt aaagtttgta tatttttttg aacaattgga ttttcataca
    16441 tattagtatt catagctggt gctataagaa cctgtgcttt tgttgccata acagtagttg
    16501 tcaacatatc gtcagctata ccatttgcaa tcttgcctat aacatttgca cttgctggca
    16561 ctattaagaa taaatctgct ttttttgcta atgatatatg ttcaacttcc caagtttttg
    16621 gttcttcaaa catatcttct actacataat tttgactcag cgattgaaat gtaagtggtt
    16681 taacaaattc tgttgctgat tttgtcatta taacatggat atttgcattt agttttttta
    16741 acttacttac aacatcacaa gccttatatg cagctatacc gccacttatg ccaattacaa
    16801 cagttttatc ttttagcatg accttactcc tcttctgaaa tttgttggtg ttgctcagca
    16861 tgatgtctag cttcttctat ctctatttcc tcttctgtca ataatctata tgttattttt
    16921 ccacttgcaa cttctttagt tgctacacat acaggctttt ccttagtttt atttgaaaca
    16981 tatggttctt ctccatcaat taattttctt gctctctttg aaacagtccc cactaaatag
    17041 tatctgttgt ctattttttc tagtacctca ttgatagatg gttttaacat aattataatt
    17101 cctccttaaa tttctctatt ataatatttt tatatctgct aactttattc ttttcagatg
    17161 atattatacc ttctatttct ttagcagatg tttttacatc ttcattaaat ataaaataat
    17221 cataatcctt aatcatttta atttcttcat atgcacagct aaatctcttc tctatttcag
    17281 cttctgtttc tgtacctctt ccaacaattc tattttttaa ttcttcaagt gatggtggta
    17341 gtacaaatat aaatactccc tctgggcaaa cttcttttat ttgttttgct ccttgcattt
    17401 caatctccag taatacatct tgtccttttt ctaaacattc cattattgcc gcttttggag
    17461 ttccataaaa attatcataa atttgagcat actccaaaaa ctcacctttt tctatcatct
    17521 cttcaaactt ttccttactt atgaagaaat aatttactcc atcaacttct ccagttctgg
    17581 gttttcttgt agtagctgat acagaaagtt ttattgtatc attttcttta agtagttctt
    17641 tgcaaatagt accttttcct gcacctgatg gtcctgatac aactagcaat aatccttttt
    17701 tcacaaacat ttaagcattc cccttctaat attgtattct tactctatat tttgtatttg
    17761 ttctcttatt ttttccaatt cgctttttac ttctactact aaattagtta tatttatatc
    17821 agatgacttc gaacctatag tgttagtttc tctattcatc tcttgtatta agaaatctat
    17881 ttttctacct atagagtcgt ctttaaatat agtattttta agttgcccta tatgactttt
    17941 aaatctaact atttcttctg ttatactgct tttatctgca tatattgcaa cttcttgtgc
    18001 taatctattc tcatctatta tacttgggtc atccagtaat tcacttattc tcaaattcaa
    18061 tttttctcta taatcttcta caacactact tgaatatttt tcaatctctt ctatatgatt
    18121 ttttaataaa tcacatctca tggcaatatc ttcagctaat tttttacctt cttcacttct
    18181 catttctctt aatttgatta atgcatcttc aactgcttga ttcaacattg accacaataa
    18241 atcttcatct tcttcctttt cttctatttt cacaatatca ggaaactttg caacattcat
    18301 aacgctaata tcatctataa tatcaaattt attttttatt tcttttagta tgtctatata
    18361 ctgagatgct aatccctcat caaaatttaa gtttacatct tctttgccta ataagtcaaa
    18421 ctttatgtat aaatctactc ttcctctttt tacataattt tttactaaat ttctaacttt
    18481 atcctctaaa aatgaaattt ttcttggaag acgtatgttt atatctgaat acttatgatt
    18541 tatcgtttta cactctacta aaaagtaata attatcatcc ttgtattctc ctcttccaaa
    18601 accagtcata cttatagcca ttaattaaac ctccaaaata ccttcacata tttttactgc
    18661 tggacctgtc atataagaga aatctcctac atctatttta actgttccac cttgagacgt
    18721 tacatttaca ctactttcta ccatattaag taaattgcac actattgctg atgcagtcat
    18781 accagtacca caacctaaag tatatccaca acctctttcc caagtataaa catgtatatt
    18841 atacttatct tctaccttta caaaatttac attcgttttt tcaggaaata ttttattatt
    18901 ttcaatctct tttccatatt tacaaacatc atctacatct aattcatcta ctataactac
    18961 tgtatgagga acccccatca acatagaagt aactttaagc tccctatcta aaacctttat
    19021 actttcttgc acaaaacgtt ctttatttgt aagtacagga acgtccttag ctataaaact
    19081 tccttttccc atattgactc ttatagagtt tatttctcta ttagtaagat ttacttttat
    19141 atcttttata ccatctaatg tatctactgt aaaactttct tttttaacta tacaattatc
    19201 ataaacaaat ttacaaaaac atctaaggcc atttccacac atagctgctc ttgaaccatc
    19261 agaattataa tataccattt ctacatctgc aacttttgaa tctttcacaa ctaacaatcc
    19321 atccgctcct acagaaaatc ttctatggca tacttttttt gctaaagcag tataatcaga
    19381 tggatttatc tggtcgaatc ttccatctat tgctataaaa tcatttccta ttccatgtag
    19441 tttccaaaat ttcataagta tcgctccttc gtaaattaac gcttaaacta ttataacata
    19501 aaaaaattcc cataaactta taatttatgg gaaaagtttt gtaatgacaa ctataatttg
    19561 aggatgtgag ggagagttgc cattacaatt agggtaatta tattatgttt ttaagctagt
    19621 tctttctatg tttatatatt aatatttcta tatgaatttt atgtatgttt tatttaaact
    19681 gtctatgaat tttttaatta aacatctggt aactagctat aatccttcta gtagatatct
    19741 attaacatac acctattaat tctttaagtg tttttaaaac atagtctaaa tcttccttag
    19801 taatatctac atgagttaca aatctatatt caccattttc cattttatta atttttatat
    19861 ttctttcaaa aaattctttt attaaaacat cctcttttat aatatcttca ctcatcttaa
    19921 aaaataccat acttatatct ggattaacat tgtttacttc aatgccatca attttattta
    19981 attcacaagc taaatatttt gcattttcat ggtcttcatt caatcttaaa gtcatttttt
    20041 caatggcaat tataccagct gctgcaacta tacctacttg tctcattccc ccacccataa
    20101 ctttttgctt tcttctagct tttgctataa attctttatt tccagcaagt atagaaccca
    20161 ttggtgcagc taaacctttt gacacacaga acataacact atcacaacac tttgctattt
    20221 ctttagcttc tacacccaaa gttaatgctg cgttaaataa tcttgctcca tctaaatgta
    20281 ctggtaagtt atgtttctca gctatttttt taatttctct taaattatca agactcacaa
    20341 ctgttccacc tgaatatgca ttttctacac atataagccc tgtctgtggt tcatgtatgt
    20401 catctcttct aattgccttt tcaacaagtt caggatttag agctccactt tcgtcttcat
    20461 ctatagttct aagctgaact ccagctatta cagcaggtgc cccaacctca tgagtcacaa
    20521 tgtgataacc tttaccaata attatttctt gccctcttag gcaatgagta aatagagcta
    20581 attgattccc aaaagttcca cttggaagga aaatagcatc ttcttttcca actttagctg
    20641 ctgctaattc ttcaagttta tttacagtag ggtcatctct aaagattgaa tctccaacag
    20701 gagcctttgc catagcttct ctcatttcgt cagtaggcat agtaacagta tcacttctta
    20761 aatcgataaa tctcattttt tcaaccttct ttcatattta tatactatag aaccttaaaa
    20821 ttgatttttt ctgtaaagtt catatacttt catagcttat actctacaca ataattatac
    20881 tatatttact ttatatttaa tctactttaa attattaatt ttaaaaataa agtttcctct
    20941 ctaataaata taaataatta tacaatttaa atattaatct ctattgttta aattccccct
    21001 agtcacaaag cgtatattat gtgtataatt aatttactga ctcattttgg tcaaagaaag
    21061 gggtgctata aatggcatta gatggattag ttatacattc aatagtcgat gaattatcat
    21121 caaaacttac aggtggaaaa atagataaga tacatcaacc tgaagatgat gaggtaattt
    21181 ttaatataag aaataataaa gaaaatttta gattggtttt aagtgcaagt gcctcaaatc
    21241 ctagagttta tcttacaagt aattatcaaa aagaaaaccc tctaaaagcg cctatgtttt
    21301 gtatgttact tagaaaatac atacaaggtg gaaatatagt agaaatttct caaataggtt
    21361 ttgaaagaat aataaaaata agtgttgaat ctttagatga attaaaagaa aagacagtta
    21421 aaaatattat gattgaaatt atgggaagac atagcaacat aataataact catggtgaag
    21481 aaaataaaat tatagattct ataaaaagag tcccttttag cataagtaga gttcgtcaag
    21541 ttttacctgg tcatgattat tcattgccac cagaacaaaa caaactcaac cctcttgatg
    21601 atatatctaa ggatttgttt attaaaaacc tagaggaatt ggaaggacct atctttaagt
    21661 ctatatattc taaatttcta ggtataagcc ctattatagc taaagaaatt tgctacagag
    21721 caggtgtaaa tcaaaatgct atcataaagg atatatctga tgaacaattt gatgctttac
    21781 acaaagtatt ttgtaattta ttcaatgata taaattctaa caaatactct ccttgcatta
    21841 ttatagataa aaaggtggat aaggtagttg atttcagttg tataaacctt acattattta
    21901 gcgatttatc atatataaat aaggatagta tgtctcgtat tctggaagat ttctatagaa
    21961 ctaaggatat taaagataga attaatcaac gttcatctga tttaaagaag agtatatctg
    22021 ttaagcttga tagactttat aataaactta aaaaacaaga agaagaatta tcagaatccg
    22081 aaaatgcaga catttataag ataaaaggag aattgattac ttcatatatt tatatggtag
    22141 aaaaaggtat ggaaagtatt gaagttgcta acttctatga tgaaaattgt aatgatgtaa
    22201 caatagaatt aaataaaaac ttgacaccat ctgaaaatgc tcaaaaatac tttaaaaagt
    22261 ataacaaaat gaaacatgct aaagtcgaga tatctcatca aatttcctta aataaggaag
    22321 aaatagatta cttagaaaac ataattttaa gtattgaaaa ctgtgaaaat ttagctgaat
    22381 tacaagatat aaaagaagaa cttgctaaag ttggatacat caagactcaa aagaaaaaca
    22441 gtaaaaagga tactatccca tctacaaaac ctcatgaatt tgtgtcttct gatggattta
    22501 aaatattggt tggtaaaaac aataagcaaa atgactatct tacactaaga cttgctgata
    22561 atgatgactt atggatgcat acaaaaaata ttcctggttc tcatgtaata ataaagtgtg
    22621 ctggtaagga agtccctgat aatactgtat ttgaaggagc tatgctagca gcattcttta
    22681 gtaagtcaaa actttcttct caggtgcctg tagactatac taagagaaaa aatgtgaaaa
    22741 aacctagtgg ttctaaacca ggtatggtta tttatgaaac taacagtact atttatgtta
    22801 ctcctgaaga agaaacagta gccaagctta aggttaaatc tgaataaatt ctgcactaaa
    22861 tacaaaataa atgttagaca actacacatt atcattctaa ataaaaatag tggccttctt
    22921 ataaagtaca ataataaggc cactttctaa ttagcttact tttaattttc taacaaaata
    22981 cttttctgtt ttaataacta tacttatatt cccaacaaat attttcagtc ttcttatata
    23041 gatgtaatta aaaagttaac actaaaagct attttttaat ttattgattt gttttagtaa
    23101 ttcattatat tctgattcat acaattcttt ttttagatta tctttttcta tagataacat
    23161 agatataaca gttgtaagtc tattttctaa tactagaaga ttttcttttt tgatttggtt
    23221 ttctctacta ggagtttttt tatttctgct aattgtatat tcgtcataat tacaatcaaa
    23281 ctcccttatt ttagcatctt ttatctctat aatatagtta caaatatgtg atacaaatac
    23341 tctatcatga gacactataa gcatagtttt atcagtattt atcagtgcct tttccagtgc
    23401 ttccatagat tttatatcta agtaatttgt tggttcatca aatactaaaa aattattatc
    23461 ttccaatatt attttacata gtgctatttt taccttttct ccaccactaa gtacttttac
    23521 ttttttgtag acatcatcac ctttaaaccc aaataggctt aagtttatcc ttataaaaga
    23581 ttcatcaaat gaactattaa ctttagtatt gtatagaaca ctcttttcct catctaataa
    23641 actttgattt tggtcaaaat aacctacttt taccttgtta tttattttta tattgtcatt
    23701 tttatcagcc aatatttctt ttatcagagt actttttcca cacccattat cacctaacaa
    23761 tgctatcttt ttacctcttt ttatcttaaa ggaaacatta tctaacagga gcttattttc
    23821 taactttaga gtcatatctt ttacctctac tagatttttg ctaattatct ccataccatc
    23881 ttgcatctta atgttcattt ctttagaaac tttaggttta ttctttacct caagtttatc
    23941 aattctgctt tttatagcct tcacatttga gtccaacttt ttcttattag tctgaccacc
    24001 cattttgtgc aacctagctt ctgaattacc cattcttttt ggtgtttttc ttatgccatc
    24061 acttaaagct tttttatata aaagagcttt ttcaagcctt tttttctctt taacatattc
    24121 ttgatactca aaatcagctc tttttacttc ttcatctttt tgcattaaat aatcagtgta
    24181 attgcctttg taagctttta gttttccatc ttctagttct aatatagttg tacataactc
    24241 atccagaaaa cgtctatcat gagatattaa taataatgct ccctcatgcc ttttaaacat
    24301 atcttccaaa accccaatgc tttttttatc taagtttgaa gttggctcat cagctattat
    24361 gatatttttc ttattactca gcgcatctgc tatttttaac ttaacctttt cccctcctga
    24421 taagtgttct tcaaatttat ctggagcatt taaaagactc ttctccttac tatgactaca
    24481 tgtttcaata ttattttcac tctgacttat ataagaatag ctttctgtaa ggtaagtata
    24541 tccttcatca atttctattt ctcccaatat tgcttttaat aatgttgttt ttccttgacc
    24601 atttgaacca actagaccta ttttgtcatt ttctaatatt tcaagtttat ctatatccaa
    24661 aattaattta tctgcataat atttctttaa attctctact tttactaaca acataaaaaa
    24721 tccctcctta acacacttaa atataaggag agattctata aataggcata acaaataaac
    24781 gtatagacta taatctactc tccttaattt aagcattgca aataaagcca ccaaaatagg
    24841 cttttcaaaa attacaatac tttaaattat aaaaaataga ttatattctc attgtcctag
    24901 ttaccttgcc ctttctttca tttattttta atcattatat caacattaat tacaaatgtc
    24961 tatagaaaat taactaattt tagttattca atatacaact tattctttaa atttattctt
    25021 cttcaacttt aaagtcctga ttatttaaaa ttctgtttaa tacaattcca acaattgcag
    25081 ctaaactaag accagttatc tttactgtac ttgttatagg gattccaaca gaaatacctt
    25141 tattagataa atatgttgtt ccaagaccta ttaataatat aactgctatg attaatatat
    25201 ttattttact ttctacacaa gaactgtccc ttatagtttt taatcctaca taagttatca
    25261 tcgaaaatag cattatacta attccaccca taactgaacc tggtatactt tgaagaaatc
    25321 caccaaactt gcctacacat gcaagcaata tagcaaatat tgctgtacgt ctaagtattg
    25381 aagggtcata gtttttagtt atagcaagta ctgctgtatt ttctccataa gttgtattgg
    25441 ctggtcctcc taaacaccct gcaactatag tcgcaaatcc atccccaagt aatgttctat
    25501 ttaatccggg attttctata aagtttttac ctactacagc accattagta gtcatatctc
    25561 ctatatgttc cataaataca gctaatacaa ctggagatat aatcactatt gctcctaaac
    25621 tgaacttagg aactgtaaag ttaggtattg caaataaact tgcttctgta atagtagtta
    25681 tatctactaa tcttaatatc aaacagatta tatacccaga aaaaactgct attaatattc
    25741 ctaattgttt tataaatcct ctaccaaact tattggttaa aattgctatt gctaaagtta
    25801 taaatgctat cacaaaattg gctgaagcca tactaaaagc tgttggcaat aaatttaatc
    25861 ctataactac tatcatagct cctgtaactt gtgcaggaaa atataattta attttattta
    25921 caccaactac ttttactata atcgacatta taatataaat taatcctgct actattatac
    25981 caccttgagc atatgctaaa tctccaccat atgcctcttt agctgctagt ataactggta
    26041 taaacgcaaa acttgaacct aaaaaggcag gtacttttcc ttctgtacaa aaatgaaata
    26101 tcaatgtacc aacaccagca cagaaaattg caactgacgg attaaaacct gtcaaaatgg
    26161 gaactaatac tgtcgctcca aacatggcta atagatgttg taaagacatt atcgtttttt
    26221 tcataaaaac ctccaaaatt attatatata aataaatcct ctgtttaatt aatatctttt
    26281 aattaattat cattccctag attctatttt ttaacctcta tatacttatt atataattta
    26341 tcattatttt acattttacg acatcaaaaa ctcaaataaa ggagaataaa aactacatta
    26401 agactcacat ccataaaatt ataatattta ttttgtgcaa tctatgtaag ctttatcctc
    26461 ctccctgact attcaatgct aagcttttat ataatactta aaaataatta ttctttgatt
    26521 gtaactgaat cttctccatc aatttctaat aaactgacag atataatttc atgtctagaa
    26581 gtaggtacat tttttcctac ataatctgct cttataggca actctctatg ccctctgtcc
    26641 actagcactg ctaattgtat tgatttaggt cttcctatat ctataatagc atctagtgaa
    26701 gctctcacag ttcttccagt gtataaaaca tcatcaacta aaactactgt cttatcattt
    26761 acattaaaat ctaaataagt tcctttaact acaggttcaa catgaatttt ttccaaatca
    26821 tctctataca aagttatatc aatatctcct gtatctactt ttgttccttc tatttgttct
    26881 attttcttag aaattctatt tgcaattggc acacctctag tctttattcc aactaaaact
    26941 agattttcta cacctttatt tctttcaatt atttcatgac ttattctagt aattgctctt
    27001 gctattgcct tttcatccat taattgagct ttttctacca tatcataatt attgtcaaag
    27061 atataatata tctatttgac aaatctactc ctttctttta attttttata aatttatttt
    27121 tatttaaaat ttcaatttaa acaacaaaaa aacttctcat cacaagacaa gaagctctta
    27181 tataaaaaat atacagtgca atttagcaat tgtcttacga tatctctgta tcgtttaaag
    27241 ataatttttt aatctatcaa atcaaattat atatttatat ttcaaatttt tcaagctttt
    27301 ttaaacaaaa tactttaata taagatattt tatttaaatt tcactaaatt tatttactta
    27361 tataaaaaca aaaaatgatt tataagtatt atacaaatag ttattttatc tttttaataa
    27421 cttccttaaa atactcaggt aattctgaat taaattcgat atattcctta gtagtaggat
    27481 gtataaatcc caacttctta gcatgcaaag tttgtccttt taatttaaat ttagtttctt
    27541 ttggaccata tacataatca cctaaaagtg ggtgatttat agacaaagca tgaactctta
    27601 tttgatgagt tctaccagtt tcaagcctag ctctcatatg tgtaaactta tcaaatcttt
    27661 ctacaacttc aaagtgagtg attgcatttt ttccatcttt tactacagcc atttttaatc
    27721 tatccttagg atttctccct ataggtttat caacagttat tttatcttct ttaaccacac
    27781 catagcatat aaattcatac tccctagtta tagtatgttc ctttaactgt tctgctagaa
    27841 aattatgtgc ataattgttc ttagctatca taagaagacc agaagtatct ttgtctattc
    27901 tatgaactat accaggtcta ataacgccat ttattgatga caacttatct ttacaatgat
    27961 ataaaatccc gtttactaac gtattttcat agtttccagg agcaggatgg acaaccatat
    28021 cctgaggttt atttattatt aagacatcat catcttcata tacaatatct attggaatat
    28081 cttgaggtaa aacttctaat actttaggtt ctggtatctc tataacaata ttatcttcct
    28141 ctttaaccag atatctagct ttttctactt tattattaac ctttactttt ccatctttta
    28201 taattttttg tatataactt cttgacatat cacctaattg ttctgctaga taaatatcaa
    28261 gtctaacttc ttcttcctct tcaagtacta aaaactcttt tacttcatcc attatatcac
    28321 ctacttttac tttcaaaaaa taaaacatat atacataaaa acacagttcc tacaactaca
    28381 aatacatcag ctatattgaa tacatattcc catataattc taaagtcgaa gtaatctact
    28441 acaaaaccta atcgtactct atcaattaga tttcccaatg caccagatat aattaatata
    28501 attcccaacc ttccaaatat atgtactttc cttgtattaa gatagtatag tccaaatact
    28561 gttgcaagta atgcaacaat tataaatatc cattgattat tttgtaataa tccaaatgct
    28621 gcacctctat tttcgacata agttaaatga aatacattat ttattattgg tattgttgat
    28681 acatccacca aattattcaa tacccatatt ttagacagtt ggtctaaacc tatgagtaga
    28741 attattatta atatatatag caatgatttt tccccctata aattatttaa aaaccaattt
    28801 tatttgaagc acactattat tattatacaa aaaaaatgct ctaaagagca actttattaa
    28861 aattgtattt tcttaataaa gctagaacaa aatttaaaat agtactactt tttttatatt
    28921 gtagatttct acatatttaa aatcatatca ttatgtataa aaaatttaaa aataccatac
    28981 taatcaatat tttttgtgta ttttatagat taaagattaa tgatgtctgt aatttaaact
    29041 tacaatcacc actaatcttc aaacaaatat ttttactttt gcacttgctt atttttatct
    29101 aaatgttgta taagttcaga tactgttacg aatttatatc ctctctcttt taatttagga
    29161 attactactt ctaaagcttg tatagtttgt gatttatcat ttcttttagt gttataatca
    29221 tgtaaaagta ttatattacc attttggacc tttgtaagga tagtatttat aattgcatct
    29281 actccagggt tagaccaatc tctagggtct aagtttgacc aaagaattat attcatgcct
    29341 ttagaaacta taatatcaca gacactttta ctcatggctc tatatggagg tctaaatatc
    29401 tttggttcta ctcctattac actttttata atattttgcg tatctgttat ttctttttca
    29461 acactactat aactattttt tgcaacattt atatgtgtaa atgtatgatt ccctatttca
    29521 tgcccctcat catattgtct ttttaataat tctttgttat attctacttt ttctccaact
    29581 acaaaaaaag ttgcttttac atcatttttc tttaatatat caagtacttg tggtgtaaaa
    29641 tcctcatctg gaccatcatc aaaagttaat gctaaaattt tttcatcctc atttcctttt
    29701 tttattatta catcttcata atcgtctttt atattgtcat ttaaactata tgcttctttt
    29761 gaactatctt gattatgtgt attatgatta tatgtatata cacctactat agataataaa
    29821 catatacata taccccatat tattttcttc atgtcattac ctcttttcta attcattcta
    29881 gcaatatatt ttataatgtc agttttttaa tctctcaata ttccattttt atttaaatca
    29941 gttaaatttg ttaggtcttc tgaataaaag ttagaatctt ctacatcagg gttttgcatt
    30001 gcatcaccta ataaattatt ttctttcttt gcacaatcat tacataaact tgtatctggc
    30061 aatatatcca atctttctaa atctatttga ttatgacata aatcacaaat cccatatgtt
    30121 ccattctcta ttttagataa tgctaagtct atttgataca atcttccctc ttcatgattt
    30181 attagactgt tttgcatatt ttgcatatat aaatctgttc ccatgtctcc tatatgatta
    30241 tcataactag ataattctcc tgatgaatac ttttcacttg tatgtttagt tgtatctcca
    30301 aataaagtat tatctttcat atcctctact aaattagtta aattttctct ttctttcaat
    30361 aatttatctt tatatttttt agtatccatt tttaacacct ctaattttaa tatttacatc
    30421 ataaaaatag tatgtggaaa agtaaattaa acatacaata aaaataagat ttgattcgaa
    30481 aaatggaatg tctcaaaatg aaatttttag ttcatttaac cctctttttt atatgaaatc
    30541 aaatatattt ttatcctttc agcaataaaa aagagactta tctctatttt gagatagtct
    30601 cttctttgaa cataatttaa atacaatttt ttaattttta tagttattat tttactaact
    30661 tgccaattta tgattatttt gttctttaat atgttttgct tttttaaaga ataaaagacc
    30721 aagaattatt gctacaataa cacctattcc attagatata ttaactggta gtctaaatcc
    30781 ttcttttgct tgcattatat aactaaatgt tactacagtc ataaaaagtg ctggtaatgt
    30841 agttatataa tggtttttat ttgctttgac taaatatgca gaaccagtcc acaacattat
    30901 cattgcaagt gtttgatttg cccaagagaa atatctccaa attatattaa aatctataaa
    30961 tgtaagagca acaccaactg cgaataatgg taaagctatt aaaaatctat ttttgactgc
    31021 atcttgatta taattcattg catctgctat tgtcagccta gcacttctaa atgctgtatc
    31081 tccagaagtt ataggacatg ccacaactcc aagtatagct aaagctcctc caataggccc
    31141 cattaatcca acagatactg tatttactac tgttgctgct ccaccatcat taaatatcac
    31201 attaagttga ggtatgccat ggaaatatga catggctgct gctgcccaaa ctaaagctat
    31261 tataccttct gcaaccattg acccataaaa tactggtctt gcctcactct ctttctcaac
    31321 acatctcgcc ataagtggtg attgagttgc atgaaaacct gatattgctc cacatgctat
    31381 tgttataaat aagtatggga ataaaggtgt tccatttgga ttcatatttt gtaaatttat
    31441 atttggtatg ttatacccct ttattattaa tcctccagct acacctactg ccataattaa
    31501 taatgctgct ccaaataaag gatatatttt accaatcaat ttatctattg gtaaaacagt
    31561 agccataata taataaagta ttatcactcc aacccatatt tgtgcgttta cacctgtcaa
    31621 tgtctttaat aatcctgctg gacctgttac aaatacaact ccaaccaata ctaaaagaat
    31681 aacactaaat attctcattg ctatttttgc accatttcct agataaaggc ctaccaactc
    31741 tgatgcagat gtacctttgt ttttcattga tagatatcct gaagcaaaat cgtgtactcc
    31801 accagcaaag attgttccaa acacaatcca taaaaatgct gatggtccaa ataatgctcc
    31861 ttgtatagct ccaaatatcg gacctaaccc tgctatattt agaaactgaa ttaaaaagat
    31921 tctcttccat ttcattggca cataatctac tccatcccta caagctatag ctggagtttg
    31981 atttttgtca ttggttccaa aaaccttttc tacaaaagta ccatagacaa aataacctac
    32041 tataagtact actattgaac ctaaaaagct taccataaat tcatcccctt agtttttata
    32101 taattgcttt ataaattttt atgagtttaa gaaaactatt tccagttcaa atagtaatac
    32161 ttttttatgc taatataaat attaattaca ttaagtgcat atttaagtac atgaaatgac
    32221 tttatatatg cattaacagc ctaaattctt ttactttgct tctacttaca gttacttcat
    32281 aatcactatt ttttaatttt aatatgtatg tattattaaa ccatggtata atttctttta
    32341 ctttggttaa gtttacgata taagacctat gtgttctaaa aaatgtttta tcatttataa
    32401 gattttcgac ttctgatatg ccttctttta atataaactt ttctttctca gtatatataa
    32461 atgtctgcct ttcattagct tcacagtaat atatatcatc aatatctatt acaactaatt
    32521 tatcaccttt ccaaaggcta attttatgtg ttatttcttc tatctcctga tttactgctt
    32581 ccttcttata tttgtataaa ttactattta cattaaatag ttcaatatca ctcatctcac
    32641 ttttttctaa tttattcagc atagaaataa ttctctcttc agaatatggt ttcaatatat
    32701 aatcaaatac atccaaagaa aaagcatcta ctgcataact ttcatatgct gttataaaaa
    32761 caattttagg tcttgattta aactgattta atgtttttgc aagtagcatt ccatcaagat
    32821 gaggtatatt tatatctaaa aaaattacat ctattttatt ttcctgaata aagtctaaca
    32881 cctctatacc attggtaaat tcacttacta cctcgattcc acttttattt tcaataaaat
    32941 atctcaattc ctttcttgct ggaaattcat cttccactat tatagctctc atacttctac
    33001 tcctttacat aaaatactat caaagtccca ttatctattc ttcttatatt aagccctctt
    33061 ccatacatca gtttaagcct taaatgtaca ttataaagcc caatcttatt ttctggcata
    33121 ttatcttgat atacattatt tataatttcc tcacttatac ctataccatt atcctctatt
    33181 gatattctca cagtattttc taatgaatac ggtaactttt ttatggatat tttcactaca
    33241 ccagctctac cactttctaa aataccatgt attattgcat tttccactaa tggttgtata
    33301 atcaaacttg gaatctttat atttacatct tctatatcat aagatacagt tagtaattct
    33361 ccaaacctag ctttttctat ctctacaaaa gatttaactt gttcaatctc tttatttata
    33421 tcaattaagt tatcactata ttcaagatta tatcttagat aagttgataa atttattatt
    33481 aactctctag ccttatctgg actaaatctt ataaatgaag ctattgtgtt tagggaatta
    33541 aacaaaaaat gtggattaat ttgtctttga agtgctttta tttctgcttt atttctcgct
    33601 tctatatctt ttttatcttt ctcaatcccc tctactatag atataacaaa tcctattcca
    33661 atctgcccaa ctattaaagg taaatatatt ccaccgatta tttgaattgc taaagaatgt
    33721 ggatatgata ttaagtatat aagtaatata gtaaaacttt cagaaatcat gcctaccaat
    33781 attccataca ttactctatg ttgttttggt atcctcttat atagaaatcc tgataataca
    33841 cctgctagta tacttgataa aagacatgga acagatgtta tccccccaat atccataaaa
    33901 tatctatgaa tccctgaaaa aactcctgct gtaataccta ccatcggacc aaataaaatg
    33961 ccacctgata ctatagatat tattctagtg tttactattg accccatata atttatacca
    34021 ctataagttc caaatatggc taaaaaagta aaaactaatg ctatacagac caagtcatta
    34081 aaactatatt cttccttttg aaatatctgc ttaaatattt ttaactttgt tattagaaga
    34141 aacaatacta caataaggct tgtcttttct attaatgcga ttaaaattga tgttttcata
    34201 atctcccttt aaaaaataaa tttttacaca ctctaaattt attatggttg ttataaatta
    34261 catatttgaa gtaatcctat cacaaaactt tccacattta atcttttaat taaataagaa
    34321 atagttgtac ttctattaac aactaatact agctagcata agcttgtaat aacttgtagt
    34381 tattattaac tttacaattc tagtatttaa ttttatactt tagaaatctc ttttcttaga
    34441 aataatactt tcgaatatca tataatacaa tattatgtag attcttactc atacaacata
    34501 tttctttaaa aattttatac tattctttgt tcaaatatct catttgattt aattgtgtta
    34561 aacacacatt atcatctctc tgttatatat ttattatata gccgtatatc gataaagtaa
    34621 ataaatccca tatttaaaat aatatgtttg acaataa
//
LOCUS       DAMJWS010000039        33905 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMJWS010000039 DAMJWS010000000
VERSION     DAMJWS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.039"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            68..634
                     /locus_tag="PC399_001979"
     CDS             68..634
                     /locus_tag="PC399_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2592839.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            939..1400
                     /locus_tag="PC399_001980"
     CDS             939..1400
                     /locus_tag="PC399_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2592840.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            1468..3714
                     /locus_tag="PC399_001981"
     CDS             1468..3714
                     /locus_tag="PC399_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592841.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            3707..5539
                     /locus_tag="PC399_001982"
     CDS             3707..5539
                     /locus_tag="PC399_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592842.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVE
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            6258..7535
                     /gene="hflX"
                     /locus_tag="PC399_001983"
     CDS             6258..7535
                     /gene="hflX"
                     /locus_tag="PC399_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2592843.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVDVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     MSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            7768..8265
                     /locus_tag="PC399_001984"
     CDS             7768..8265
                     /locus_tag="PC399_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2592844.1"
                     /translation="MDAETLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            8315..8839
                     /locus_tag="PC399_001985"
     CDS             8315..8839
                     /locus_tag="PC399_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2592845.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            9151..10515
                     /locus_tag="PC399_001986"
     CDS             9151..10515
                     /locus_tag="PC399_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2592846.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLVAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            10654..11820
                     /locus_tag="PC399_001987"
     CDS             10654..11820
                     /locus_tag="PC399_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2592847.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            11937..12719
                     /locus_tag="PC399_001988"
     CDS             11937..12719
                     /locus_tag="PC399_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2592848.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(12838..13491)
                     /locus_tag="PC399_001989"
     CDS             complement(12838..13491)
                     /locus_tag="PC399_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2592849.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(13515..14879)
                     /locus_tag="PC399_001990"
     CDS             complement(13515..14879)
                     /locus_tag="PC399_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2592850.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            15538..16968
                     /locus_tag="PC399_001991"
     CDS             15538..16968
                     /locus_tag="PC399_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2592851.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            17071..18174
                     /locus_tag="PC399_001992"
     CDS             17071..18174
                     /locus_tag="PC399_001992"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2592852.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            18189..18425
                     /locus_tag="PC399_001993"
     CDS             18189..18425
                     /locus_tag="PC399_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2592853.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            18442..19491
                     /gene="vorB"
                     /locus_tag="PC399_001994"
     CDS             18442..19491
                     /gene="vorB"
                     /locus_tag="PC399_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2592854.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVIEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALTSKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            19493..20233
                     /locus_tag="PC399_001995"
     CDS             19493..20233
                     /locus_tag="PC399_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2592855.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            20236..20775
                     /locus_tag="PC399_001996"
     CDS             20236..20775
                     /locus_tag="PC399_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2592856.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            20899..22998
                     /locus_tag="PC399_001997"
     CDS             20899..22998
                     /locus_tag="PC399_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF2592857.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIENRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(23265..23780)
                     /locus_tag="PC399_001998"
     CDS             complement(23265..23780)
                     /locus_tag="PC399_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF2592858.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(23872..25083)
                     /locus_tag="PC399_001999"
     CDS             complement(23872..25083)
                     /locus_tag="PC399_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2592859.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            25759..27201
                     /locus_tag="PC399_002000"
     CDS             25759..27201
                     /locus_tag="PC399_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2592860.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            27243..27596
                     /locus_tag="PC399_002001"
     CDS             27243..27596
                     /locus_tag="PC399_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592861.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            27787..28608
                     /locus_tag="PC399_002002"
     CDS             27787..28608
                     /locus_tag="PC399_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2592862.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            28866..30749
                     /locus_tag="PC399_002003"
     CDS             28866..30749
                     /locus_tag="PC399_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF2592863.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            30974..31654
                     /gene="pgmB"
                     /locus_tag="PC399_002004"
     CDS             30974..31654
                     /gene="pgmB"
                     /locus_tag="PC399_002004"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2592864.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            31651..>33905
                     /locus_tag="PC399_002005"
     CDS             31651..>33905
                     /locus_tag="PC399_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097546124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2592865.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGKKY"
BASE COUNT        13199 a         3706 c         5967 g        11033 t
ORIGIN      
        1 tgtgtatata ttgtacttta atagtgatat aataataaaa aaatttttaa agagggtgat
       61 attacatatg agaaaacttg aagagatatt agaatataat aaatcatttg tagagaacaa
      121 agaatatgaa caatatgtga catcaaaaca tccaaataaa aaaatagtag tcttatcttg
      181 tatggataca agattaactg agcttttgcc taaggctatg aatttgaaaa atggagatgt
      241 gaaactaata aaaaatgcag gagctactat aatgcatcct tttggaagta ttatgagaag
      301 tgttttagtt gcaatttacg aatttgatgt agatgaggtt atggttgtag ggcatcatgg
      361 atgtggtatg tgtaatgttg atacggataa gttacttggt aaaattttag atagaggaat
      421 atctaatgat gtaatactta cacttcgtag tgctggaatt gatacaaaac aatggttaca
      481 tggattcgat tctgcagaag aatctgtaaa agatagtatt gatttaataa aagggcatcc
      541 tttaattcca gatggtatta ttgtacatgg tctaattata tcacctgaaa ctgggaagtt
      601 agatgttgtt gtaaatggat atgaagataa gtagagtatc ataatgttat ataatcaaga
      661 tactgttgaa taagatgttg tttcaaaatt tattttggga caactttttt atttgaaaat
      721 atatgaaaag gtttatttta agctttacaa taaaaaatat aagtagctga ctaattaaaa
      781 atcttactat tatttattaa aattataact atatgatata aaaatattta ataaatgaaa
      841 tttatatgaa ttttttataa atatattgaa aaaatgcatg cagtatagta aaataatgct
      901 tagccgacta agtaatataa aaaataagga gggcaagtat gaatagcaat tctgaagaaa
      961 aattaaatga tgttttcttg caattaatgc gaatatattt tataagaact catttttatt
     1021 ttgataaatt aggcttatat catggtcaac cccaaatact tttttcttta aagatgaaag
     1081 atgggcaaag tcaaaaagaa ttagcagata aaaggaaagt aaaagcatct acaactacag
     1141 ttatgataaa gaggatggaa aaagctggac ttgtggaaag aaaacaagat gaaaaggacc
     1201 aaagagtatc taggattttt ttgacagaca aaggtttaga aatatgtaat aaagttgaag
     1261 aatttaacaa ggaattggaa agagagtttt ttttaggatt tagtgaagaa gaaaaaatag
     1321 tacttagaag acttctaatg caagtacgag ataatttaaa agcaggatgt cataagcatg
     1381 aagatgaaaa atatttttaa agtttaaaat aatgtttttc aatttaatgt tattagacta
     1441 attaatatta cagaagggaa gaaatgtatg tttaaattaa ttaaatattt aaagaaatcg
     1501 gcattatcaa tagtaataat agtctgtcta ttggtaattc aagcggtatg tgacctttct
     1561 ttaccagaat acacatccaa tatagtaaat gtaggtatac aacaaggtgg agtagaaaac
     1621 tctgttccat ctgtgattag agagagtgaa ttaaataaaa ttactttatt tatggataaa
     1681 agtagtaagg ataaagtact agataattat actttgctga ataaaaagga ttatgttaag
     1741 tataaagata agtacccagg tctaaaagat gaatctcttt atgagttaaa tactaaagac
     1801 aaagacacta tagatgattt aaatgttatt tttggtaaag ctattttaat agtatcaggg
     1861 cttgagggcg atagtaaaga ggtaaaagct atgaaagctc agttaatgag taaacttcca
     1921 ccacaagcta ctcaaagtgg agatgttgat atatttaaat tgttatcagc aatgccaaag
     1981 gaacagttag atacattgac taaagagatg agcaaaggct ttgaaagtat gccagagagt
     2041 atgataacac aaagctctgt ttcatatgta aggtcagaat atgagaaaat aggtattgat
     2101 acagaaaaaa ctcaaaataa ttatatatta tttactggtg ctaaaatgct tggtatagca
     2161 atgattagta tggtagctac aataacagta ggatttttag cagcaagagt agccgcaagt
     2221 gtaggtagaa atttaagaag tggagtattt agaaaagtta tgtcattttc aaatactgaa
     2281 atgaatgaat tttctacagc ttcattaatt actcgtagta caaatgatat tcaacagata
     2341 caaatgttga tggttatgtt gcttagaata gtattctatg cacctataat ggctataggt
     2401 ggagtgataa aggttttaaa cacaaatact tcaatggcct ggattatagc agtagcagta
     2461 gttgctatct tatcattaat tgtagtgtta ttctcagtgg ttatgcctaa gtttaaactg
     2521 gttcaaaagc ttgttgataa gttaaactta gtaagccgtg aaataattac tggtattcca
     2581 gttatcagag cttttagtac tgaaaaacat gaagaagaaa gatttgataa agcaaataaa
     2641 aatttaacta aaacaaatct ttttgtaaat agagttatga catgtatgat gcctgctatg
     2701 atgcttataa tgaaccttat cacagttctt atagtttgga gaggtgcata cagtgttgat
     2761 tctggagcaa tgcaagttgg agatatgatg gcatttatac aatatacaat gcaaataata
     2821 atgtcattct taatgatatc aatggtatct attatgcttc caagagcatc cgtttcagct
     2881 acacgtatag atgaagtatt agtgtcagat ataagcataa aagacccaga aaaatcagag
     2941 gcacaagagt ttaaggaaga taaaaagggt cttgttgaat ttaaaaatgt ttcgttcatg
     3001 tatccagacg cagaagaacc tatattaaca aatatatctt ttacagcaaa gccaggagaa
     3061 acaactgcct ttataggaag tactggtagt ggtaagtcaa ctcttataaa tttaattcct
     3121 cgtttctttg atgttactga aggagaaata ttacttggtg gagttaatat aaaaaatgtt
     3181 tctcaacatg atttaaggga aaaaattgga tatgtacctc aaaagggagt attgttttct
     3241 ggtactatag aatccaactt aaaatatggt agaaaagacg caacagatga agaaatgaga
     3301 acagcagctg aaatagctca agctacagaa tttatagatt ctaaaccgga agcttttaaa
     3361 actgaaattt ctcaaggtgg aacaaatgta tctggaggtc aaaaacaaag actttctatt
     3421 gcaagagcaa tagcaaaaaa tccagaagtt tatatatttg atgatagttt ttcagcactt
     3481 gacttaaaaa ctgatgcagc tcttcgtaag gctttaaaat caaaaactgc tgaaagtaca
     3541 gttctaatag ttgcacaaag aattagtact atcttaaatg cagaacagat aattgtttta
     3601 aatgagggcg aaatagttgg aataggaaca cataaagagc ttctaaaaaa ttgcgaagtt
     3661 tatgagcaaa tagctttatc acaactttca aaggaggagc tagctaatga gtagaatggg
     3721 aagaggtcct atgggaaaat ctatgggagc aggacaaaaa gcaaatgact ttaaagggac
     3781 tatgcgaaaa ctaattgcat atttatctaa atttaaaata tcaataattc tagtaattgt
     3841 atttgctatt ggtagtgcgt cgttttctat agtaggtcca aaaattttag gaaaggcaac
     3901 tacaaagata tttgaagggc tagtgagtaa agtttctgga ggaaacgtag gtatagattt
     3961 taatgcaatt ggaaaaatct taaccttttt acttttcctt tatttgataa gtgcactgtt
     4021 ctcttttata caaggattta taatgagtgg aatttctcaa aaagtatcct ataatttaag
     4081 aaaagagatt tcagcaaaat tggatagatt gccaatgaag tattttgata ctaagacgca
     4141 tggtgaaata ttatcgagaa ttacgaatga tatagatact ttaaatcaaa gtttaaatca
     4201 aagtatgaca cagcttataa catcagtaac aactatgata ggtgttttga taatgatgtt
     4261 atctataagt ggaattatga cattagtggc agttttaata ttaccaatat caatgtttgt
     4321 aatttcaaga ataataaaga aatcacaaaa atactttaga tatcaacaag aatatttagg
     4381 aaatgtaaat ggtcaagttg aggaaactta cagtggccaa acaatagtaa aagcatttaa
     4441 tcgtgaggat gaagtaattg aagagtttga taaactaaat gatagcctgt ataattcagc
     4501 gtggaaatct caatttttat caggtataat gcaaccactt atgatgttta ttggaaactt
     4561 gggatatgta atggtatcaa tcttaggagg ttggcttgca attaagaaaa caattgaggt
     4621 tggtgacata cagtcattta tacaatatgt aagaaacttt acacagccaa tgactcagat
     4681 tgcacaggtt gccaatttat tgcaatcaac agcagcagct tctgaaagag tatttgagtt
     4741 tttagaagag gaagaagaag tccaaattgt tgaaaatgca gtttcaatag atggtttaga
     4801 aggtaaaatt gattttgaaa atgtcaactt tggatataac ccaaacaaga ctataataaa
     4861 tgatttctca gtaaatgttg aacctggtca aaaagttgct attgttggac ctacaggagc
     4921 aggtaaaact acaatagtta aactgttaat gagattttat gatgttaata gcggttcaat
     4981 actgattgat ggtcataata ttaaagattt taatcgtagt gagcttagag aaatgtttgg
     5041 tatggttctt caagatacat ggttatttag tggttcaatt atggaaaata taagatatgg
     5101 taagttgaat gctacagatg aagaagttat agaggctgct aaatctgcac atgttcatag
     5161 atttattaag acattaccag atggatataa gatgaaatta aatgaagaag caagtaatgt
     5221 ttctcaaggt caaaaacaat tattgactat agctcgtgct attttagctg atcctaaaat
     5281 tttaatatta gatgaagcta caagttctgt cgatactaga acagaagtat tgattcaaaa
     5341 agctatggat aatcttatgg aaggtagaac aagttttgtt atagcccaca gattatcaac
     5401 tatacgtgat gctgatatga ttcttgtaat gaatgaagga gatatagttg aacaaggaaa
     5461 tcatgaggaa cttctaaaga aaggcggatt ctatgccaat ctttacaatt ctcaatttga
     5521 agaagatgaa gcaatgtaac aaaaaactat cttagcatag acatgtaatt aataagataa
     5581 aataaatgag ggtgccttaa aatgaacttt ttaattcata aggcactctt ttgtgtatga
     5641 aataaagtgt atttttatca ttttaacaat gaaaaagagg attctctata ttttgagata
     5701 gccacataat tttatgtctt tacaaagtgt ttattttatt ataatatgta gtaaggaaaa
     5761 tgttggaggt gattatcaaa atgtatattt aattttattc tttaaatttt attataaata
     5821 gaatttaatt ttaatagttg atgattatat tgaagtatat gtttttatta cttttattaa
     5881 tcatgcaaat aactacttaa atttaagaat agggttaaat ataaaatgat aattacatat
     5941 aagttaatta aactaatgta tttttaatat atatttagaa ttttaatcct tcttatgata
     6001 taagtagctt gttttgtatt acttagattt gtaaggagtt tttttattga aaaaactgca
     6061 taatatattt gggaggaggt gtaagaaagt gattttttat ataagattga gaaaataatg
     6121 cacgattatg gttattataa tagtaaataa gaagtgtcat ttaaggataa gataaaaagc
     6181 aaaattcttg atgaatttaa ttatagagcc ataatctcgt gctatagatt aaataaaaaa
     6241 tagcagataa ggagaatatg gatatggcga aaaaaggaat aacagtagga ttaaacataa
     6301 ataataaatc tgaggacttt aatgaactaa tgattgaact tgaaaactta tgttcagcat
     6361 gtgatattga tgttgtaggg agtataactc aaaatgcaaa gcaggttaat agagcatttt
     6421 acattgggac aggtaaagtt gaggaaatat taaacttaat aaagaaagaa aatatagaga
     6481 tagttatatt ttacaatgaa ctttctacat cacaactaaa aaatcttgaa gaaaagctta
     6541 attgtgaaat tatagataga acggcattga tacttgatat atttgcacaa agggcaaaaa
     6601 ctagagaagc taaacttcaa gtagaagtag caagtttaaa atatatgtta ccaagactta
     6661 taggttcaaa tgaaaattta ggacgtcaaa gtggtggagt tggtactaaa aatagaggtt
     6721 ctggtgagaa aaaattagaa cttgaccgta gaagaataga agaaaagata acatctttaa
     6781 atagggagtt ggatgattta aaattccaaa gagaaactca aagaagtatg agaagaaaat
     6841 caaatcttcc aaatgtagct ctagtcgggt atacaaatgc tggtaaatct tcaataatga
     6901 ataaactagt agatatattt aaaaactctg aagaaaagaa agtgtttgag aaaaacatgt
     6961 tatttgctac acttgaaact tctgtcagaa atatagttct tgcaaataac aaagaatttt
     7021 tattatctga tactgttggt tttgtaagta atctaccaca tgacttagta aaagcattta
     7081 ggtcaacatt agaagaagca tgtgaggcag acgtactgtt acatgttatt gatatttcaa
     7141 atccaagcta taaaagtcat attaaagtga cagaagatac tttaaaacaa ataggtgcag
     7201 atggtatacc tatgatacat gtgtataata aaattgattt aatagatgta gacgtattgg
     7261 atagaatttt agatagtatt gataaagaag gtatatttgt ttcagttaaa aaagatataa
     7321 atatagataa aatgataaaa tgtatttgtg atagcatatt taaagattat gtaagatgta
     7381 agttcttaat tccatatgat aaaggtcatg tagtttctta ttttaacgaa aatatgagca
     7441 ttataaatac agaatacaga gaagatggag ctattttaga tgtagaatgt agcaatatag
     7501 aatataataa gtataaaaag tatgctttag agtaaatttt gaaaaactta ttatagccta
     7561 aaatatattt aatttataat ttattataaa ttgattgtaa atatgatttt atgaaacggt
     7621 atagccataa tgctatgccg ttttgccatt tataataaaa ataaatttat tgacataata
     7681 gtaactaatt actataatat aaagtaatta gttactattt aaaattatca aaaaattaaa
     7741 atctaagata aagagaagtg tataataatg gatgcagaaa ctttaatgaa aaaatatatg
     7801 gtagatgatg atagtagaaa acaagctata tttgcaagta tatttgtaat gcaaaataaa
     7861 cttcaaacta catgtgataa attagaccca gaattaacaa tgaaacaatg gttactttta
     7921 gctattgcgg actctgtaga tgaaactctt acattaacca aacttggaga attaatgggg
     7981 tgctcaagac aaaatgtaaa aaaactagct attgctttag aaaagaaagg ttttatacaa
     8041 attgagcaga atgaaaaaga ttcaagagct atctgtcttg tgacaaaaga tagagtgcaa
     8101 gagtattcta aaaaagtaag aggtatgcag gaaaaagtat tacagctttt atttgaagat
     8161 tttacagaga aagaagtgga acagttattt tttggaatta tgaaattaag tataggaatt
     8221 gaaaatgtga aaaattatgt agaaaataat gacgttggag aataagcaag tatcaaatca
     8281 actttgtatg acatcagaga gggggataat ttatatgaaa aatgtagtag tgatttataa
     8341 gagtaagtat ggttcaacaa aaaaatatgc taaatggctt tcagaattat tgtcttgtga
     8401 tatttttgaa actaagaata ttaatataga aaaggttatg aagtatgata cgattatttt
     8461 aggaggaggt ctttatgctt gtaatatagc gggaatttct ttcttaaaaa aaaattataa
     8521 agaattgcag aaaaaaaatt tagtagtatt tggcgtagga gctgctccat atgatgatga
     8581 agtaataaag aaattaagag aagtaaattt taaggatgaa ttaaacaaaa ttccttgctt
     8641 ttattgtcgt ggggcatggg atgagacaaa aatgtctttt atagattata ctctttgtag
     8701 tttttttaaa aaatctatag ctaaaaaaga acgtcaaggt cttaaaccag cagaagcacc
     8761 tttattgtct ggagaagcat ttgattggtg taataaagaa aatttaactc caatacttga
     8821 ttatataaga gaactttaag aggtatatat ttataattat ttattatcta aagttagatt
     8881 tcatagaaac ttactagaca ttatttataa aataatatta ttaaagatgg ataatttaat
     8941 tgattcaata aggttataat tttaaaaaat gtttatttta aaataaatta ttttataagc
     9001 ttacttatac ttaataaggg gcttttttta ttggaagatt tatgttatat tgagatgaat
     9061 ttattatgat ataaaaaata atgttaggat aaatttaaat ctttttctaa aaatgtatta
     9121 atataattct taaagaaagg atttttaata atggatataa aagagttagt taaaatgcaa
     9181 agaaaatatt ataatacagg taaaacaaga gatatatcgt ttcgtataga acagttgaag
     9241 aaacttaagt tagttgtatc acaaaatgaa gaaaaaatac ttttagcact aaagaaagat
     9301 ttaaataaat cggattttga aggttttatg acagaagtag gcatgtttta tagtgaatta
     9361 aactttgcta taaagaatat aagaaaatgg agcaaaataa aaagagttaa atcaagtatg
     9421 gttaattttc catctatatc caaagttgtt ccacaacctt atggtgttac tctcataatg
     9481 tcaccttgga attatccatt tcaattagca cttataccat tagtttggtc attagtagca
     9541 gggaattgtg ttatattaaa accttctgaa tattctgtgt caacatctag tgtagttaaa
     9601 gacatagtgg aagatacttt ttcaaaagaa tatgttgcag ttgttcaagg aagtcaagaa
     9661 gaaagtgaaa agttactatt ggaaagattt gattatatat tttttacagg aagtactaat
     9721 gttggtaaaa taatcatgaa atcagcatca gagcatttaa ctcctattac tttagaactt
     9781 ggaggaaaaa gtccttgtat cattttaaaa gatgctaata tagaccttac tgccaaaaga
     9841 cttacttggg gaaaactaat aaatgcagga caaacctgcg tggctccaga ttatgtttta
     9901 gtgcatgaag atagaaaaaa tgagttaata gagaaaataa aatattatac taataagtat
     9961 tttggagata atccatgtaa taatgagcaa tttcctaaaa tcataaatca gagacatttt
    10021 aatcggatat tatctcttat tgataaagat aagatagtct atggtggaaa ttataataag
    10081 gagacattaa agatagagcc aactatagta gacaatgtta attgggatga taatataatg
    10141 aaggaagaaa tatttgggcc tatatttccc attttaacat ataaagattt agatgaagta
    10201 atacaaaaaa ttatacaaat gccaaatcca ttggcacttt atatttttac aaaaaataag
    10261 tacctagaaa ataaactttt agaaatgatt ccagcaggcg gatgttgtat caatgataca
    10321 gttactcata tagctacgaa ttacttacct tttggtggta ttggtgaaag tggtatgggc
    10381 tcatatcatg gaaaagctgg attcgataca tttactcact ataagagtgt gttaaaaaaa
    10441 ttaaatttgg atgtccctat tagatatgct ccatacgata ataaattaat taaggttctt
    10501 aaaaagataa tgtaggttat attgtagaat aataaatata aataaaattg agtatttgta
    10561 ttctgaatat attttatttt taatttggta tatttgttat aaaagtggtg attttgtgat
    10621 aaaatttaat tatatagaaa taaggaggaa aaaatgaaaa ttaattggaa tacaaaatac
    10681 acaacaatag caatttatac atttataata gctgctagta gtattatatt ttatctagta
    10741 tcatcacaaa ttgatgtatt ttctaataat ttagatgcta tatttacaac attacaacct
    10801 tttataatag gatttgctat tgcatatctt cttaatttta ttttaaagtt ttatgaagac
    10861 agaattttta taaaaagtga gaaattaaaa aaactaaaac aaagttcaaa aagaggcttg
    10921 ggtcttttat taacatatgc aacagctaca ttgatacttt acttatttat gcatttcgta
    10981 ttgccacaag taattgagag tattgttgga ctagcaaatg atatacctat gtatgtaaac
    11041 aatgcaacta agcttattga taaattaatg actgatttaa atcttgatga gcaatacttt
    11101 aatttggcag ttgataagtg gaatgagttt gttacatata ttataaagtt tgtaacagat
    11161 ttaatcccaa tattaggaag tatgttaaag aatgtagcct ctagtatatg gaatattgta
    11221 ctaggattaa ttgtttcagt atacttatta attgataaag aaaaattcta tggattaagc
    11281 aaaaaaataa cttatgcagt atttacagaa aaacaagctg ccagaatttt agaattaact
    11341 catagaagta actatacatt tggaagattt ttaggtggca aaatattaga ttcattcatt
    11401 ataggtattc taacatttgt aatacttacg ttagttaaaa tgccatatac attattaata
    11461 tcagttataa ttggtattac aaatattatc cctttctttg gacctttatt tggagctata
    11521 ccatctacgc taatagtgat gtttgtatct cctataaaag ctttctggtt attgttgatt
    11581 atattaataa tacaacagat agatggaaat attataggac ctaaaatact tggagattca
    11641 attggtatat ctgcattttg gattttgttc tccttattgg ttgcaggaaa attattaggt
    11701 tttattggaa tggttatagg agttccaatg tttgcagtaa tttattctat aattaaggat
    11761 attgttgaaa gtaaattgga taaaaaggga ttaccgacag atacttccga ttatatgtag
    11821 ctaaaaaata ttatgtataa ttaaaaatat ataaattaag caatatttaa agcactgaga
    11881 aagttaaact cttggtgctt tttagatata aaattattat agaagaggtg ttttttgtgg
    11941 catttataga atgtaaattt aaatctaaat atactggagg agaaactgat ataattgtaa
    12001 ttttgcctgt agcaaatgga aaagaacttt ttagtggtaa agagatatat gattttgaag
    12061 gtaaatttaa aacattatac ttatttcatg gtttagaaga tgaccaaagc tcatggatta
    12121 gatatactaa tgtagagcat tatgctaaaa ataaaaatat agccctagta ataccaagag
    12181 tagaaactag tttttatacc gatatggcgt atggacacca atattttact tttgtaagcg
    12241 aggaattacc taaatttgtt agagctatat ttcctttatc agataaaagg gaagataact
    12301 ttgtagctgg tatgtctatg gggggatatg gagctttaaa attagcgctt tcaaaaccaa
    12361 aagagttttc ggcagttgca agtttttctg gttcgcctga tatgattcat gagttagaaa
    12421 atcaacaaat agaaaccaat gcagacattc tatttcacga ttttggtaga ccagaagacg
    12481 ctagaaatag tgagaatgat ttactttaca tacttaagaa ccttaaagaa agaaatgaag
    12541 atattcctaa aatttatcaa ttttgtggtg atagagattt tttatataaa aataatcaga
    12601 tgtttaaaaa atatgcagat tctctgggtg tagatgttga atatgaagag tgtgtagggg
    12661 ggcatgaatg gcgtctttgg aatttatgga taaagaaatt tatagatata attggatagt
    12721 acacttatgg tattaacagg tatactataa aatatcttta aatatgataa agactgaaat
    12781 cctaaaatca aattatgatt ttaaaatttc agtctctaag aatttaataa aattttatta
    12841 agttcttaca cgagttatta ttggagtaaa attgtcaact atatctatat agtatttact
    12901 caattcctct aaaatctctt cgcataaatc cgtactatga ccaagattaa taagaaactg
    12961 ttttgtaaaa tgctttatta cagcttgaga agatatatcg tacttgtgtg aagtataaac
    13021 tctaattatt tcaggtttca tagcaagtat accaatacaa gaaagtatta attcatcatg
    13081 ttctatagat tctttatcat taacatatct tttcatgacc atttcggtta cacttctttg
    13141 atagcttaaa atagcttctt cataagctat atccaaataa aaatttctat atgcttcgtt
    13201 ttttagaagg aaacaaaggt cagcattcat agcaagaaag aacatcatca tagtaccttc
    13261 ttcatggtag ttatagatta aagactgcat ttcatttctt atatcttcat atattatagc
    13321 tcctatttcg cttttacttg agaagtaata gcttattaga cctaaattta tattagctct
    13381 ttctgcaatt tgcctagttg ttgtcttatg ataaccttgc tcaaaaaata attctctagc
    13441 agcacttata atactagctt ttgtttcttt gcccttttct gattttgaca tatttactgt
    13501 gtatacctct cttttcaatc tttttttgaa aatttgctaa ctataaatat tgtaatagaa
    13561 ccacctatta taggaaccaa aattctgtca aatacagtaa gaataccaat catatttaat
    13621 aatgccaata taacacttac tataacagca tagatagctc ctttttttgt agcacctttc
    13681 cagcaaagac caattacaaa tggtgcaaat attacacctg tacataatgg agctggccaa
    13741 aacaataatt ctaataaact atcaaattta agtgctatga ttgcacctat tatagctgtc
    13801 aaagctgatg caatttgaga atactttaga gtatttttta aatcattaaa ttctatatta
    13861 ggatgtaata atttgttata aatatctctt gatataatag cactcattga ggtcattaat
    13921 gaatcggcag tagacattat ggcagccaat atagcagcta agaataatac agatattaaa
    13981 ggtggcaaag tagtcgatat catttgaggt aaaacctgaa cagatgatat atttggaaat
    14041 aaactaccac caatagctcc aattattcct atagggaaag caagaaataa tagtataaat
    14101 cctgcaatta cactactttt aactgcattt ttagcattat cagcacattg atatcttaaa
    14161 aaactttcat aagaaaatat tgagcctaaa actaatggta aaactgtata cataaaatca
    14221 ctaatcgatt tatttcctgg aaagaatggt tcttttacca ttgattgtaa cataagtaaa
    14281 gggttgtcgc caactaattt taagcttata taagctacta ttggcaaaaa cactatacaa
    14341 atagcaagtt gaataaatgc tgatactgca actccccaca ttcctgatgt ataagtatat
    14401 ataatgataa ctataactcc tataattaca gctatatcat aaggaatacc aaatgcagta
    14461 gatacagaac caaaagaaat caattgacta cccataatag ctagtataga tattggtgtt
    14521 acaagacttt gataagctct taaaaataca ctatgaccat atctagtttc aatataatca
    14581 gcaggtgtta tatcacaaga taagcttctt aattttcctg cgaaaaatgc atttataaag
    14641 caagctaaag ctgcacctat agtagaatac attccaaccc aaagtcctct ttcagctccc
    14701 cattgaactc caccaactaa gcaccctcct ccaaaagatg tagcagcaat ggttgcagct
    14761 agtataggca aaggcagatt tctacctgcg attaggaaat cggatgctgt tttaactttt
    14821 tttgaaaaat aaaaaccaat ccatatgtat attgcacacg taacaattaa aagtaacata
    14881 gtcttttctc ctttataaaa tattttaata aaatatacaa aatatattta aaatatattt
    14941 tatataaaag tagtacgata tttgttttat tggttttaga ttaatatagc tataataatt
    15001 tatgatatta gatggaaatt acgtaaaaat agaaaattga aaattctgaa atttttgtat
    15061 attgatattt atctcataga ttcttttttg taattgtaac acgcttttat ttttaaatca
    15121 atagtacatg tataaaaata aaaggaattt aaataagaaa aagatatttt taatatctgg
    15181 atgtagtata gaaatatctt gaataaatta ttcttgaata aaattataaa aaattaaaat
    15241 cactagaaat atgataaaat aatttaaaat acgcatttca atgacatttc aaaacaataa
    15301 aataataaaa aacaaaccta aattaagtct aaatagcttt ttagttaatt ttagtagtaa
    15361 aaaataaaac ttaaaaagtg tttgacaatt atgaattttg agaatataat taaattaaaa
    15421 ttatacacat ataaaaatat actcctgaat caagaatgag atatttaaga ttcaaaatat
    15481 atattattat acgtgaataa taaaaaagta agaaaatttt ataagggagg ggtagatgtg
    15541 agtagtcaag aaagcaaacc aaaaggaaat cttatatcaa attttagaga tgatatgaga
    15601 gaccctaaga aaagagcaat atacacatat tcaagttcta taaacttagc gtacaatttt
    15661 ataggttact ttgtatcaac tgttacaaat atttttttaa ctgatgtggc aaaacttggt
    15721 gtaattatgg caggtggtat gcaaattata caatctttca taaaaattgc tgtagctcct
    15781 ataacaggta cagtatttga caaacaacca ttcaaaaaag gaaagtatta tccatggctt
    15841 agatatgcac cagcagcgtt agcattaaca tatggaatat ttttcatagt tccattaatg
    15901 aaaataagtc cagcattact tttacctttg atgacagctt tattattaat agcatcttgt
    15961 gctcagcaag tagttttcac tataatttat gctatttacc ctgtagttgc taagacacct
    16021 agagaccgag ttataggctc aactactacg aatatattta aagaagttgg taaattctta
    16081 gtaggttttt catatccgtt attattagtt ttatttgcag atgtattagg tggagaagga
    16141 atgggttatt taggtaccta cttattcttt gcagcaggat gccttttagt attttggttt
    16201 tcttcaacag agataataaa atctggagca gaagaagaag ttcttcaaaa agaagagaag
    16261 ccaaagatta aagcaagtga aatggcaaaa gctttattca ctaatgtacc acttatgatg
    16321 gcattttgtt tagaatttct tatatgtata agatctatag gattaggacc tttggctcca
    16381 tattatttta aatatgtaat agaagatgaa cgtggattag cagtattttt atcaatcatg
    16441 cctttggtaa gtgtagcatt catgtttttc gcaccagtgt ttataaaaat ctgtagagag
    16501 caaaaactag cttcaataat ttctttttct atatgtgcac tatgccactt attggttgca
    16561 tggactcctt gggggaaaac tactttagga gtaactatgt tgctggcaat tggtggaggt
    16621 ttttcaaatg tagtatcaat aataaatctt aatttctttg ccggttcttg tgattatggg
    16681 cattggaaat caggaaaaga cttacctggc ttaagtatgt cactatatcc agttgcaata
    16741 caagtaggag tattacttgc aactactata agaacagttt taatgaactc aatgggatat
    16801 caagctgata tggtagtcac agaagcagtt aaatcaggtt ttataaatat gatttcttat
    16861 tctatggcaa tacctttaat aatagctgtt gttatagcaa tactatatcc agtaagtgac
    16921 aagaaattaa accaaataag agaagagtta aatcaaagaa atgcttagtt aaaagaaagt
    16981 taagtagatg gttaatttaa gctattaaag taagtagacg caatagcgtt tatataaatg
    17041 aaaatacata tacagagggg gcttttaaaa atgatagaaa aattattgag aaataccgta
    17101 aaagaattac atcaatatgt tccaggtgaa ccaatagaaa aagttaagga aaaatatggt
    17161 gtaaaagaaa taataaaatt agcttcaaat gaaaatcctc taggaccatc accaaaagca
    17221 atagaagcta tgattgaaat gttaaaacaa ggtcaacttt atccagagcc agaagctaac
    17281 gaacttagaa gaaaattagc tgaaaagcta gatttaaaac cagaaaattt tattgttgct
    17341 aatggtgcag ataacgttat cactctaata ggtgaggctt ttataaatag aggagatgaa
    17401 gttatatatt gtaacccaac atttccatca tatagaactg caactataaa aaatgaaggg
    17461 ataccagtag aagtaccttt aacagaagat tataagtatg atttacaagg tatattagat
    17521 aagattacag ataaaacaaa attaatatgt gtatgtaacc ctaataatcc aacaggaaca
    17581 attgtggatg ataaagagtt agaagaattt ttaaagaaag tccctgaaaa tattataact
    17641 atattggatg aagcatatat agaattttta acagttccta attatgtgga tggacttaag
    17701 tatgtaaggg aaaattataa cgttatagta acaagaactt tctctaaaat atatggtctt
    17761 gcagggctta gagtaggata tggtattgct aaagatgaga taataagaac tttatttaca
    17821 gtaaaagagc ctttctctgc aaatcgtgtt gctataagtg gagcaacagc agcacttgat
    17881 gatgaagagt ttataaaaga aacatatgaa ttaaatagag ttggaatggc ttactttaaa
    17941 gaagagttta caaaaatggg atttgatgtt gtagattcac aatcaaattt cctttatgta
    18001 gatatgaaaa cagatatacc taagttattt gaagacttga aaaaaagagg gtttgtaata
    18061 agaccaagtg ctacacatgc acgtgtaagt attggaacta tggaagaaaa taagaaattt
    18121 gtagcagttt taaaggaaat attaaatatt aaaggagagc caagtattgg ataatagata
    18181 gaaatattat gaaacggggt gtagatatga acttaaaaat aaacgcagag agatgtaaaa
    18241 gttgtgaata ctgcgttata tcttgtaaaa aaggagcatt aaaaatatca agtaagataa
    18301 acaaagaagg ttatgctcat gtagaagttg atgagagtaa atgcatactt tgtggaattt
    18361 gctatcaggt gtgtccagat aatgtttttg aaataattaa agaagaagca tgtcaagagg
    18421 cttagaagga gttgggataa tatgaaaaag atgtggaagg ggaaccacgc cattgcagaa
    18481 gcagccctaa gaggaggttg tgaattttat gcaggatatc caattacacc acaaacagag
    18541 gtaattgaat ttttatcaca tagaatgtct gaacttggaa gaacttttat acagtccgaa
    18601 aatgagatgg ctgctatata tatggtatat ggagcatatg caagtggaat gaggtctatg
    18661 acgagttctt caggaccagg aatatcttta aaacaagaag ggatttctta cttatgtgca
    18721 aatcattacc cttgtgtaat cgtaaatgta cagagatggg ggcctggtct tggctcattg
    18781 gactcagcac agacagacta tctaagagat actagagggg gaggaaatgg agattatcat
    18841 ttgatagttt atgcaccaaa cagtattcaa gagactgtag atttgatgta taactcattt
    18901 gatgtagcgg agaagtacag agtgcctgtc gaaattttga cagaagcagc tttaggtcaa
    18961 atgatggaac cagtagaatt tccagagttt aagaagagag aaggagattt aggctggact
    19021 tatgatggaa gtaatagaga ccatgcaaag gttgcagata accaaaagcc aactttttgt
    19081 atggagaaac gtatgagaat aactgaaaat gaacaacaat gggaagatta tcaagtagaa
    19141 gatgcagaat atgtgtttgt agcgttcggt ataccatcaa gaacaactat gaatgctgtt
    19201 agaagactta gagaacaagg tgaaaaagtt ggaataataa gaccaataac agtatggcct
    19261 ttcccatata aagcatttga aaaggtaagt aaagatgtaa aaggatttat ctcagtagaa
    19321 tcaacagaca caggtcaatt agtagaagat gttgctttaa cctcaaagaa agtatgtaaa
    19381 gaaaatgtac ctgtgtatgg tttatttagt ggaaatcata taccaaaaac attgcaagta
    19441 atggacactt atagcaaaat aaaatctggc gaaatcaagg aggtgttcta atatggctac
    19501 agaaaaatta acacctagcg ttgtttcaat accaaacaag ttttgcccag gttgtggtca
    19561 tggaatagta aatcgtataa tagcagaggt aatagaagaa catggatatg aaaaaaatca
    19621 tgtattaaca ttaggagttg gttgtgtctg caatatgaat tttagctgga atggtgataa
    19681 gatgcaaact gcacatggta gagcttcatc tacagctata ggagttaaag tagcacttcc
    19741 agatacatta gtaatgactt accaaggtga tggagatgct tatgttatag gtctttctga
    19801 aactttaaat acagcatata gaaatcataa tgtgactgta tttgtaataa acaataataa
    19861 ttttgcaatg actggtggac aaatgtcatg gaccactatg ccgggtcaag tcacaacaac
    19921 aagtgtaaat ggaagagata taaagactac tggtagtcca ctgaaagttc ctgagatggt
    19981 ggcaaacttc tttgatgtag catatgtagc aagaggttca gttcatagcc caaaggaaat
    20041 aattaatcta aaaaaatata taaaaaatgc aatagaagct caattaaatg gagagggata
    20101 ttccttagtt gaaatattag caccttgtcc aactaactgg ggtgtatcac tagagaaatc
    20161 tatcaaatgg atggaagaag aaatagtgcc atattatgct cttggcgaat ttaagcaaag
    20221 ggatggtgaa tagttatgga aaaagagcta gtatttgcag ggtttggtgg tcaaggagtt
    20281 ttaactcttg gtctgattgt agctgagtca gcacttgaat tggggaaaca agtaacttgg
    20341 atgcctgcat atggtccaac tatgagaggt ggaaaagcct atagtgtagt aaagttttct
    20401 gatgagagta taggtgggcc tgatatggaa gaaatagatg ttttagtagc aatgaataaa
    20461 ccatctttag attatataaa tttagttaaa gaaggtggaa cagtagttat taatacatct
    20521 gcaatagatg agaatgtaac attaagagaa gatataagtg tagttaaaat aaattgtcag
    20581 gaattagctc aaaaagtaaa caatccaaaa gcagcaaata tagtagtact tggagcatta
    20641 attgctaaaa cagggttatt tgaaaaagaa ttagctttaa aaactatgtg tgatttcttt
    20701 gaggaaaaag gaaaaggaaa gtttaatgtt caaaatgaag cagcattcat ggaaggctat
    20761 aataacgcca tttgataaat tacccttatc ttaagtgatt tatctcaaga tttaataatc
    20821 tttagtgcca agagaaactt catcttgaga taaaattcac ttaaaatcac aactataaac
    20881 atataggagc gtgaatttat gagtttggaa aaacttatta gaccaaaaag tattgctata
    20941 gtaggagtga ctgataaact tggttttgga agaagtgcag cacttagtat agtaaaaagt
    21001 aaggaaacag atagagtata ttatgttaat cccaaaagag aagaattgtt tgggagaaaa
    21061 tgttataaga cgatacaaga agttccagaa gtagtagact gtgtagtagt atgtacacct
    21121 agaaatgtag ttccatctgt attaaaagat tctggagagt taggaacaaa ggcagcagtg
    21181 gtatatgcta gtggatttgc agaggaaggt acagaggaag gtacagattt agaaaatcaa
    21241 cttatagaaa tatcaaatac atacgatatg aaaatattgg gtccaaactg catggggttg
    21301 ctaaattgta tagataaggt taatctatgg gcaggaggtt ctaaatggga tttagatact
    21361 aaaaaacctg gtataggaat tgtaggtcaa agtggattta taactgctga aattgtttca
    21421 agtgattatt ttaatatatc ttatggtttt tcaacaggaa atggaaatat agttacacta
    21481 gaagatgcag tagatttcct tgtagatgat gattatgcat cagtaatagc aatttacctt
    21541 gaaggtttaa aaaatcctca aaaatttata gatgctttaa aaagggcagc acaaaaaaga
    21601 aagccagtaa taatattgaa atcaggaagg tcagaaaaag gtgctatttc tgctgcttct
    21661 catacaggaa atttagctgg ttcgagtaaa gcttttgaaa gtatttttga aaaatatgga
    21721 gtaatttctg tggaaaattt agaacagttt atgtgtttag cacaagcttt tagtgtttta
    21781 gatggtaatt tacctgcaaa ttctaatttt gcagcaataa acttctcagg aggagaaaat
    21841 actatttgtg cagatttagc agaagaaaat ggtgtagaac ttgcagaaat atcttctgaa
    21901 actaaagaag aaatgaaaaa atatcttcca ggatttgcaa cacctaaaaa tccactagat
    21961 gctacaacag cattattcca tgaaaaggat atgatagtag gtttattaca tacctttgaa
    22021 aaggatgata gtgttggatt tactatggta ggtgcaaata taagagatga agaaaatgaa
    22081 atgcatgaaa ccctttgtaa agctgtaagt gaagctagag aacaaggatt gaaaaaacct
    22141 gtgtttgcag ttcctacact tgaaggaaat agatatttag attataggac tagattagaa
    22201 gataatcaag tgcctataat gtcatcagtt ggaactacat ttacttgttt taataaaatg
    22261 gctaagttta tagattatga ttattcaaaa agaactttag aatttaaagc tgttaagaaa
    22321 agagagtcaa ataatgtagt agcattatca gaattagata gtaagattga gatgaaaaaa
    22381 tatggagtac cagttccagg acaaggtaat gcaaaaagta ttgatgaatt agatgaaatc
    22441 ttaaaatata ttaagtatcc tgttgtttta aaaataaatt cttctgaaat attgcacaaa
    22501 tctgatgttg gaggagttaa aataggaatt gagaatagag atgaggcagt agatgcatat
    22561 aatgagattt taactaatgt aaaaaaagca aaaccagatg ctaatattga tggtatatta
    22621 gttcaagaaa tggtagaaag tggtatagaa attattattg gtataactaa tgatgaccaa
    22681 tttggaccaa tgttattagt cggtttagga ggagtatttg tagaggtatt taaagatact
    22741 acattatatc cattacctat aaatcatgat gaagctatta tgatgcttaa aaaactaaaa
    22801 tcatttaagc ttttaaatgg ctatagagga agtgagcctt gtgatattga tgctcttgca
    22861 gatatgatgg tcaagttagg aaaatatgct tatgaaaata aggatgaagt caaggaaata
    22921 gacttaaatc cagtgtttgt atatcctaaa ggaaaaggcg tatgtgcagt agatgcctta
    22981 atagttaaat ataaatagtt tataagtttt ggtattcaac cataattaaa gcaccgactg
    23041 aaaatcacat tttgtaagtt agatagtgtg attttcagtc accccaaatt taatttaaaa
    23101 atattttaaa atgaaagtat agttttaagt ttaattgtga aatagcactg tataaacaat
    23161 aaaatactac gtataaaacg aataataaaa ggtgttttaa tgataagaat caatcttgat
    23221 tttcattaaa acaccttctt tattatatta aaaattttta aacgttatat tgtcacaggt
    23281 gggtttgttg gagctgcata tgtacgatta gcaaattctt ggtcaacaaa gaatagacca
    23341 ccctctccag caagtttaac acgttttata ttgtcttcac aatttgtttc ttcttctgct
    23401 tgttcagata taaaccaaag taagaattga gaagttctaa agtctgattc agagtttgct
    23461 acagctgcta agttgtttat taaagctgta accttttttt catgagctaa cgtacgctca
    23521 agaatatcca ttgcagaagt aaagtctgaa tctggcattg ggatagcatc aagctgaacc
    23581 tttcctccta cattatgtaa gtatttgtaa aaaaacatag cgtggtctgt ctcttcttta
    23641 tattgaacat aaaaccaatt agaaaatcct ttaagtcctt ctgcttcaag gtaagaagac
    23701 atagaaagat aaagatacgc agaatataat tcatggttaa tctgctcatt aagtaatttt
    23761 tccattttag ctgaaatcat ttttatacct ccaaatttta aatgctctta taaaatataa
    23821 taatattaaa tggaaaaata ttcaataaaa gtgtttaaat attttacata tttaataaaa
    23881 atttatacta ccattaaaaa aattataact tacaatggct atagagccaa ttaatggtgc
    23941 atttgctcca aaatttgaat gccttacact tattttttta tatttagaaa aacttgctga
    24001 agcagtcaat ttttttaata atgtattttc tataaatgta ccaggtatat taaagtcata
    24061 gcctattatt atagaactac aatcaatcaa tgtaagtgta ttaactaatg catatgatac
    24121 gtatgtacaa aattcatcaa gcaaaaacat ggcaagacta tctttattat ttgcctcatc
    24181 tattaacttg actaaagaaa aatctttaca gttaattaat ggggagttag gataaatatg
    24241 gctcaatgat tcaattcttt taattagatt actgacactt gtataataat ctaaacatcc
    24301 attattacca caactgcaca atggtccact aaaattaatt gatgtatgtc ctatttctcc
    24361 actttgtcct aggttgccag tatgaagttt attttctaga actagaccag ctccaatacc
    24421 attcattatg tgtaggtata tataatttga tatattttta cctaaaccat acattttttc
    24481 tgctaatgca cccgaattag catcatttat caaaaatatt ggcaaatcag acatttcttt
    24541 tataaaatct actatattta ggtttgatac atttccaaaa tctgggggat ttagtatggt
    24601 accatttata tcatctactg gacctattga agaaatacca attccaatta tatctctctt
    24661 gttgattttc attattttag taaacattct ttttattata tcttttaaaa catcatttga
    24721 tagacatttg gggtaagttt cagaaagttg ttttacaata ttacctttta aatctgaaat
    24781 aattacctta catatatttc ttcttattag gattccacaa atacaaggcg atatattaga
    24841 aatatctaat aaaataggtt tacgacctga tgaatgtgca tttaaagttg atgactctat
    24901 ttccctaatc atattttcat taataagttc agtaactaga tttgataaag tcattttagt
    24961 aagtcctgta gacttagcta aatcaactct ggacataggt gtatttgtag aaagtaattt
    25021 caatattatt aacttatttt taatttttac atctttgatg ttagaacctt gtttgttact
    25081 catcataaat ctccttaaat aaagttaagt gttttattta ttttcaagta tactatcacc
    25141 ttataataga gtaacatttt tttacttgaa aatatcatta attagaatat atcacattat
    25201 atatcataaa atatataaaa aaatgttaga attaaattaa aagtgaaaag tagattgata
    25261 tataaatagt aaatacttaa ttaaaaaaag ataatgatag caataattat tgttaccaag
    25321 aatctactac ttcaagtgtt accttaggta tttaagttga tagtggtttg cacgtaataa
    25381 acttgatgta atatatattc ttaataatat cgataaaaga tttacttaaa aataatatag
    25441 attatgtaaa catataatct atttattgta tacaataaaa tttcattttg taaaacagtg
    25501 aattaaatat gtaatataga tatcgatttt tattacaaat tgttactaaa tataggtaca
    25561 ttagaacaaa aatctagtaa attgtaacta aaatgtaaat ttttccttta atctatacaa
    25621 tattatggaa actttttgta atttatgata taataacaaa tatataatta ttaaattata
    25681 ataaatgact atagcgtaga tgtaatacta tgttatagtc atttttgaat taaacttgat
    25741 atatcaaggg ggaagaatat gagaacaatt agaagagttt tagcattagg tttaacatta
    25801 gctatatttc taataaatgt acctaatgta gatgcattaa catcagatac aattaaaggt
    25861 aacaatatat atgagacggc tggattaata gcagataaaa agagttatga tacagcaatt
    25921 atggtaaata tggataattc tatagcagat ggtctttcag caagtggtct tgcaggtgct
    25981 gttgatgctc caattttgct tgcacaaaaa aataagatac caaatgaaac aaaacaaaga
    26041 ttaaaaaatg ttaaaaagat atacataata ggtaaagaat tatcaataag taagtcagta
    26101 gaaactgaac taaaaaacac aggggcacag gtaactagat tgggtggaga tgacagaata
    26161 aagactagtt atagtgttgc taaagaggta aatggtatca aaaaagttga tgaagtgata
    26221 ttaactaatg catataaagg agaagcagat acaataagtg ctgcacctgt atcagtaaga
    26281 gatatagctc ctatagtact tacagatgga aagagtgtac ctttttcaac aagcaatgta
    26341 aaaacatatg cagtaggtgg gagtatatca atgagcacta gtttagttaa taaaactaat
    26401 gctaaaagac ttggtggttc agatagatat gatacaaata agaaagttat aaaagagttt
    26461 tatccagatg catcagaatt ttatttgagt gatggatatg atttagtaaa tgcacttaca
    26521 ggttctacaa ttgctaagga aaatccaatt gtattggtgt cagaaagtag tgataagtct
    26581 atattagcag gagcagataa gattactaga ttaggttcaa taagtgacag tgtgtataat
    26641 aagtgtgttt ctgctgcaca aaataatggc gattcgtcta caaaaggtga gtcacctatg
    26701 aaaaatgaga caagcatatt aggtcagcca acagctagtt tagaagcgtg cttaaaatgg
    26761 gcaaaatcta aaaaagctaa tgatttattt atagagttaa taccaatatt atatgatact
    26821 gctgttcagg aaggtgttaa tcctgttttg gcagtagctc aatctgcaaa agaaactggt
    26881 ttttgtaatt ttggtggagt attagatgca tcatttaaga atccttgtgg acttaaaact
    26941 tctgtaggag gttctgatac tgataagaat gcccattcaa gatttgatac ttgggaagaa
    27001 ggaatattag ctcaaattca acatttatgc ttatatgcag gacaagatgg ttacccactt
    27061 tcaaatccag tagaccctag acatgaaaaa tctttatttg gtaaggctaa aacagttgaa
    27121 agtttatcta ataattgggc tggaggtcaa tatggacaag atttagtaag aatgatggga
    27181 gaaattgagg caacaaaata attgaagatt atcaaatatc caataaataa ggattgatta
    27241 ttatgaaaaa gagtaatatt tttagtatca tatttcctac agtcataatg gtgctaatgt
    27301 ttttaacttt tgctacagat atatttaata ttccagatat tcaatctaaa agtatttttg
    27361 ttgtgggaat ggttttgatt tttccaattt cttttttgat acaagggata atttgtgtat
    27421 taaataagac taattggatt ttatctctta ttgtgtcttt agtaacttat atagtattaa
    27481 tgtatatatt tttaaatgat tctgcatata tatatgtttt actttataca gctttttata
    27541 tgattggaca tataacagct aaactctatt acaaaattaa gacttttaaa aattaaattg
    27601 aagtatataa aagtaacatt aggagaagta tccttaatga ttttctaaaa tcagaaatag
    27661 gtgcttctct tttttgtaaa aataaaagta tgtaaataaa atttaaccat tataattttc
    27721 ttttaacaaa tgtatacaat aaacttgttg tataattaag atagataaaa taaagggagg
    27781 gataatatga atttaataga taatcatatt catactaatt tttcaagtga tggaaaggat
    27841 tccatggaag atacaataaa aaaagctata agtattggtg ttagatattt gacttttaca
    27901 gaccatttag aacatgatga agatagagga ttttgcataa attataatga ctatgttcca
    27961 gtatttaata agtttaaaga aaaatataaa aaggacatag agttattact aggtgttgaa
    28021 gtgggttaca gaaaacatct aaaaaatgaa atagaagaaa taataaattc taatcctttt
    28081 gattttgtat tgtgctcaac tcatacaatt gacaatgtac ctgttccatc aaaggagtat
    28141 tttaaaggtc ttagcaaaga agatgcgtat tataaatatt ttaatagcat acttgaaact
    28201 aatcgtgaat ttggagatta caatatatat ggtcatttgg attatatatc taggtatgga
    28261 atatattcag ataatagagt tatatacaat gactttaaag atgttattga tgaggtctta
    28321 aagtcaataa tcaataatgg gagtggtata gagctaaata cttctggtta tagatatgga
    28381 ttaaatgcta tacatccaaa tgaagatatt ctaaaaagat ataaagaact tggaggattt
    28441 atagttactg taggttctga ttctcatagg gtagaagata tatgtaaaga ttttgatgtg
    28501 gcctatgata tgcttaagta tcttgacttt aaatatgtat ctttatttaa agaaagagaa
    28561 acttattttg taaatataga aaaagttaaa tctaacaata tagcttagag tgtgttttga
    28621 agtaaattaa tggatagtta aaaagttaat agttataata ttgtatcctc tagaaaaata
    28681 ctagaggttt ttattttgct aaaataacta aatagaatta aatttaaata gtatagttta
    28741 ttttaaattg aatagtttat tgttgatata ttttactatt atatattttt attgtgatat
    28801 aatagtaaaa tatattaacc tatctttaga ttatataaat tattataaag gagggaaatt
    28861 ttaaaatgtc taataaaaat aaagaaaata taactttaag taatcaaatc aaaggaacaa
    28921 ttcaaaatag tttaacaaaa gaagctatat tacatatacc aatgagcaat tatgcctatg
    28981 gttatgatag agaaacacta catataagac ttagaactaa aaaaaatgaa gcgaaaagag
    29041 taattttacg aataggagac caatatgtgt gggataaagg aggtgcagga ggaggaaatt
    29101 taaatgcatc tggagttggt tggtctggtg gaacaaatat agtaatgagt aaagaagtag
    29161 aaactgaatt atttgactat tggatagcta agtgtaagcc tttaaataaa cgctcacgat
    29221 atggctttat aatagaaggt caagaggaaa aacttttatt tacagaaaaa agaataatag
    29281 agttgggaaa tgaaaatgat gaaaaggaat tatgtgaaat aggtaatttt tttggatatc
    29341 catatttaaa ttatatagat atacctaaag tgccaaattg ggtgaaagat actgtttggt
    29401 atcagatatt tccagataga tttgcaaatg gtgacccgtc tataaaccca gaaggagtag
    29461 ataaatgggg agctattcca accagagata attttacagg tggagattta caaggtgtga
    29521 tagaacactt agattatttg agtgatttgg gaataaacgg aatttacttt tgccctatta
    29581 caattggaaa aaccaatcat agatatgata cggtagatta tatggaggta gaccctacat
    29641 taggagataa agagacttta aaaaaactaa tagaagaagc gcataagaga aatataaaaa
    29701 taatgttgga tgctgttttt aatcatatag gatattattc taaacagtgg caagatgtag
    29761 ttcaaaataa ggaaaattct cgatataaaa actggtttta tataaaagat atgagtaaaa
    29821 ttgatactcc aatagaacaa atagatgaaa aaaatatacc ttatgaaacc tttgggtgtg
    29881 aaaaatatat gcctaaactt aatacagaaa attcagaagt aataaaatat ttattggatg
    29941 taggcaagta ctggatacag gaatttgata tagatgcgtg gagattagat gtttcaaatg
    30001 aagtagacca tgttttttgg agaaagttta gagaagaagt caaaaaagta aaaccagaca
    30061 tttatatatt gggagaaatt tggcatggta gtttaccatg gctaatgggt gaccaatttg
    30121 attcagttat gaattacttg atgtcagaag ccatgaaaaa attcttttgt acagatgaga
    30181 taaacgccga agaatttaag tatatgataa atgatgtaat ggtaagttat ccaatacaag
    30241 ttagtgaagt tatttttaat ttacttggaa gtcacgatac tacaagaata ttgacttatg
    30301 ctaatggaaa tattgataga tttaaacttg catatatgtt tatgtttgta caaacaggat
    30361 gtccttgtat atactatggt gatgaaatcg gaatggaagg agaattaagt tcaatatctg
    30421 aaggtcaaag aaaatgtatg gaatggaatg aagaaaaatg gaataaggat atatttgatt
    30481 ttatgaagaa gattattaga cttcgtaaag aaaataaaga acttaggtct atatcaaatg
    30541 aatgggtgtt agcagataag gaaaatggaa cgattatatt gaaaaaagaa tctatttcta
    30601 taataataaa taattcatct aaaaatataa ttttgacgtt accagattat ttagcaaata
    30661 aaaaagtaaa agatttatat gaagaagaaa tagttgattt aaaagaagaa ctaatgttag
    30721 agaaatacaa gttcattatt ttaaaataat acaaaaaagt atttatttta gatttcatgt
    30781 taaattatta tacagtttaa ataagtcttt ataaacgata aaaaaatatg gtctacaagt
    30841 gagtgaattg aaagtagatt ttaaattaaa gactgagtta gagtagataa ttttataaat
    30901 aatatcttaa atacaagata gaaaataaat tgattattag tctaaaaatt tcttgtagaa
    30961 agaggaaata agaatgattg aagcatttat atttgattta gatggtgtaa ttacagatac
    31021 tgcatattat cactatatgg cttggagaaa actagcccat aaagttggga tagatataga
    31081 cacaaaattt aatgaatctt taaaaggcat aagtagaatg gagtcattgg acaggatatt
    31141 agaatttgga aataagaaat attcattttc agaagaagaa aaagtgagaa tggctgaaga
    31201 aaaaaataat tactatgtat cattgataga tgagataact tcaaatgata ttttgccagg
    31261 tatagaaagc ttgttaattg atgtaaaatc taataatata aagataggtc tatcttctgc
    31321 aagtaagaat gcaataaatg tattaaatca tttgggtata agtgacaaat ttgattttat
    31381 agctgatgca ggaaaatgta aaaacaataa accacatcca gagatatttc ttatgtcagc
    31441 gaaagggctt aatgtaaatc cacaaaattg tatagggata gaggatgcaa gtgcaggtat
    31501 agacgctata aactcagcta atatgttttc tgttggtgtt ggaaattatg aaaacttaaa
    31561 aaaagcaaat ttagttgtag attcaacaaa tcaattaaag tttgaatata tacaagaaaa
    31621 gtataatgag tatatagtta ggagaattat atgaaatgtt tatttagtaa tgtagatgag
    31681 tggaagatta tacaagataa gattgaaatc aaagaaaata gattagcaga gtccataatg
    31741 agtataggca atggatatat gggtatgaga ggaaactatg aggaaaatta tagtggagat
    31801 acacacaagg ggtcttatat agctggagta tggtttccag ataaaactcg tgttggttgg
    31861 tggaaaaatg ggtatccaga atactttggt aaaattccta attcagtcaa ctatataggt
    31921 gtaagaattt tcatagatga tgtagaatta gatttagcaa aatgtaaagt agaagatttt
    31981 tatagagaat tagatatgaa aaatggtatt ttaaatcgta ggtttattgt aaatataaat
    32041 gataaaaaat ttgaagttag ggttgctagg tttttaagta tatctgttaa ggaacttgca
    32101 gtaattaaat acagtataaa atcattaaat tctaatacta aaataaaatt tgtaccatat
    32161 ttagattcaa atataaaaaa tgaagattca aactatgagg aaaagttttg gaatggaatt
    32221 tcttcaagtt ctttagaata taaaggtagt attctttctc gaacaaagga gaatgcattt
    32281 aatacaccag tattcactgt gggttcaatg atgtatatta agacaaatgg tgatgtagaa
    32341 gctgtatctc atgagagtga ttataattac tgtgaaaatt cgataacaat ggatgtttta
    32401 aaaaatgagg ttgcaagtat agaaaaatat gtatctataa cttcaactag agattataag
    32461 gaaagtgagc tattaagaaa atgtgaagac attttaaata gagaactatc taaaagttat
    32521 gatgatatat taaatgaaca tacaaagtta tggaataaaa gatgggaaag tgcagatgta
    32581 aaaataaatg gagataaaag tagccaacaa ggtataagat ataatttatt ccaactattt
    32641 tcaacatatt atggagaaga ttcgcgatta aatataggtc caaaaggatt tactggtgaa
    32701 aagtatggag gagctactta ttgggataca gaagcgtttt gtgttccagt atatctaggt
    32761 gtagcagata aggaggtaac tagaaatctt ttagaatata ggtacaatca gttagaacag
    32821 gcaaaggata atgcaaaaaa actagggctt aaaggtgctt tatatccaat ggtaacattt
    32881 gatggagtag aatgtcataa tgaatgggag ataacatttg aggaaatcca ccgaaatgga
    32941 agtatagtat atgcaatata taactataca aattatacag gtgactataa atatataaaa
    33001 gaaaaaggaa tagatgtaat aatagaaata gctagatttt gggcaagtag agttcatttg
    33061 tctacaaaaa aagatttgta tatgatacat ggagtaacag gtccaaacga gtatgaaaac
    33121 aatatcaata ataattggta tactaattat atagctaaat ggtgtttaga ttatgctgta
    33181 gaaaatatct tacgattaga gaaagagtgt aatgaatgta ttgacagaaa taatgtgact
    33241 agggaagaag ttgagttttg gaaaataata tcaaataaaa tgtatctgcc gtatgatgag
    33301 gaactacaaa taataattca aaatgataat tttttagata aagaatttgt agaagtaaag
    33361 gatttaccaa aagaaaattt tcctcttaat caaaattggt cttgggataa gattcttagg
    33421 tcgtgtttta taaaacaagc agatgtatta cagggaattt actattttgg aaatagattt
    33481 actaaagaag aaaaaaagag aaattttgac ttctatgaaa aatatactgt acatgaatct
    33541 tctttatctt ctagtattta ttctattatt gcctcagaaa tagataacct agaaaaggct
    33601 tatgaattat attcaagaac agctaggctt gatttagata attacaataa tgatacaaat
    33661 gatggactgc acataacttc aatgtctggt tcatggcttt ccattgtaca tggatttgca
    33721 ggcatgagaa catggaatga aactctatct tttgacccta agttgccaaa agagtggaaa
    33781 aattattctt tcaatataaa ttatagagat aatattatta atgtagatgt atgtagtgat
    33841 ttaattaaaa tagaaaattt aaaaggtaga ccaataaaaa ttattgtata tgggaaaaaa
    33901 tatat
//
LOCUS       DAMJWS010000040        33677 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMJWS010000040 DAMJWS010000000
VERSION     DAMJWS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.040"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            90..1154
                     /locus_tag="PC399_002006"
     CDS             90..1154
                     /locus_tag="PC399_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF2592866.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            1312..1998
                     /locus_tag="PC399_002007"
     CDS             1312..1998
                     /locus_tag="PC399_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2592867.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYKDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            1988..3019
                     /locus_tag="PC399_002008"
     CDS             1988..3019
                     /locus_tag="PC399_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2592868.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITGDLKLIALLL
                     IYTMVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            3101..3766
                     /locus_tag="PC399_002009"
     CDS             3101..3766
                     /locus_tag="PC399_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592869.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            3779..6196
                     /locus_tag="PC399_002010"
     CDS             3779..6196
                     /locus_tag="PC399_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2592870.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSST
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(6377..7168)
                     /locus_tag="PC399_002011"
     CDS             complement(6377..7168)
                     /locus_tag="PC399_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2592871.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(7201..7788)
                     /locus_tag="PC399_002012"
     CDS             complement(7201..7788)
                     /locus_tag="PC399_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2592872.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYSNSKSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(7965..9356)
                     /locus_tag="PC399_002013"
     CDS             complement(7965..9356)
                     /locus_tag="PC399_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF2592873.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(9546..10844)
                     /locus_tag="PC399_002014"
     CDS             complement(9546..10844)
                     /locus_tag="PC399_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF2592874.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGYPLPIPYIMPLKETLCMS
                     MSRGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(11201..12076)
                     /locus_tag="PC399_002015"
     CDS             complement(11201..12076)
                     /locus_tag="PC399_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF2592875.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(12269..12955)
                     /locus_tag="PC399_002016"
     CDS             complement(12269..12955)
                     /locus_tag="PC399_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF2592876.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(13004..13537)
                     /locus_tag="PC399_002017"
     CDS             complement(13004..13537)
                     /locus_tag="PC399_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2592877.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(13581..14213)
                     /locus_tag="PC399_002018"
     CDS             complement(13581..14213)
                     /locus_tag="PC399_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2592878.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(14303..15382)
                     /locus_tag="PC399_002019"
     CDS             complement(14303..15382)
                     /locus_tag="PC399_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2592879.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(15515..16861)
                     /gene="pepV"
                     /locus_tag="PC399_002020"
     CDS             complement(15515..16861)
                     /gene="pepV"
                     /locus_tag="PC399_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF2592880.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(16930..18210)
                     /locus_tag="PC399_002021"
     CDS             complement(16930..18210)
                     /locus_tag="PC399_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF2592881.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(18337..18597)
                     /gene="hfq"
                     /locus_tag="PC399_002022"
     CDS             complement(18337..18597)
                     /gene="hfq"
                     /locus_tag="PC399_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF2592882.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(18711..19646)
                     /gene="miaA"
                     /locus_tag="PC399_002023"
     CDS             complement(18711..19646)
                     /gene="miaA"
                     /locus_tag="PC399_002023"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF2592883.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(19684..21651)
                     /gene="mutL"
                     /locus_tag="PC399_002024"
     CDS             complement(19684..21651)
                     /gene="mutL"
                     /locus_tag="PC399_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF2592884.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(21662..24505)
                     /gene="mutS"
                     /locus_tag="PC399_002025"
     CDS             complement(21662..24505)
                     /gene="mutS"
                     /locus_tag="PC399_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF2592885.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(24666..25400)
                     /locus_tag="PC399_002026"
     CDS             complement(24666..25400)
                     /locus_tag="PC399_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2592886.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(25369..26382)
                     /locus_tag="PC399_002027"
     CDS             complement(25369..26382)
                     /locus_tag="PC399_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2592887.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(26404..27174)
                     /locus_tag="PC399_002028"
     CDS             complement(26404..27174)
                     /locus_tag="PC399_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2592888.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(27149..27439)
                     /locus_tag="PC399_002029"
     CDS             complement(27149..27439)
                     /locus_tag="PC399_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF2592889.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(27570..27669)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      28289..28378
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            28431..28607
                     /locus_tag="PC399_002030"
     CDS             28431..28607
                     /locus_tag="PC399_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592890.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(28722..29078)
                     /locus_tag="PC399_002031"
     CDS             complement(28722..29078)
                     /locus_tag="PC399_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592891.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            29334..30247
                     /locus_tag="PC399_002032"
                     /pseudo
     CDS             29334..30247
                     /locus_tag="PC399_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            30478..31089
                     /locus_tag="PC399_002033"
     CDS             30478..31089
                     /locus_tag="PC399_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF2592892.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            31387..31725
                     /locus_tag="PC399_002034"
     CDS             31387..31725
                     /locus_tag="PC399_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2592893.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(32132..33568)
                     /gene="miaB"
                     /locus_tag="PC399_002035"
     CDS             complement(32132..33568)
                     /gene="miaB"
                     /locus_tag="PC399_002035"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF2592894.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT        11464 a         5276 c         3860 g        13077 t
ORIGIN      
        1 attgaaaata taactttatt cttatataat taaattatac aacaataaag ttatattttt
       61 taaatatatt tttactaggg ggaatagaat tgaaaagggt tgatgagaga aatactatgt
      121 ttgcaagagc aaattacaaa aaagatagta ctgcttataa tgactattat aagaaaaatc
      181 ctgacaaaaa agaaattgat gatagtataa gaaatagacc aaatttatgt agtgaaggaa
      241 caatgactta caatgagtta aactctccaa tggcaagtag tgcattcgat tttttatctg
      301 atatcaaatc tttatgcgaa ggaaaagttt ctgatactaa agtagatgta gatgcaaagt
      361 caatgacaaa aaaaattaag ggtcttgcaa agcagtatgg agcttctgta gttggtataa
      421 ctaaattaaa agactatcat atttatacaa ataggggtag acatgaagaa aattacggtg
      481 aagaaattaa tctaactcat aagtatgcaa ttgtatttgg atgtgagatg gataaagaaa
      541 tgataaatag agctccaatg atatgtgaag ttatagaaac atcaaagtgc tatgtggatg
      601 catctatcgt cggcatgatt ttaagttact atataagaaa tttaggatat gatgctagaa
      661 atcatatgga tgctaattat ttagtaatgc ctgtttttat agctcgtgat gctggtttag
      721 gagatatagg aagaaatgct atacttacaa ataaagatta tggctctaga cttagattag
      781 gtgttgttac tacagatatt cctcttttag aagatgaata tgttgatttt ggactagaag
      841 atttttgtaa ggtctgtaaa aaatgctctt ttaactgccc ttctcattcg ctttctaatg
      901 atattaaaat tggtgatgat ggaaaatata attgggtaat tgaacatgag acatgctata
      961 ttaaatggag atatttaggt acagattgtg gtatgtgtat atctgcttgc ccatttagcc
     1021 aaaatctcga aactataaag aatactactt catttaaggg taataatgag cttattcaaa
     1081 aagctttaga tgagtatact tcaaaatttg gtaaaagaat atttatacct ggaaatccat
     1141 cttggttgaa ataattttat ttaataaaga atctgtacta gaatttatca taggacaggt
     1201 tctttattaa atatataaat ttttaaatac tttttctcgt ttataacaat tcagcaatgt
     1261 ttttagtcta taattgacat tataaaatat taaactaaaa ggagtaactg tatggaaaaa
     1321 atattagtag tagaagatga ttctatatta aataaaactc tatcatataa cctaattgaa
     1381 gatggatata taattacttc aaaatttaca gctaaatctg cactaaagtc tatttttgaa
     1441 tgtgaatttg atttaataat tttggatatt aatttaccag ataaaagtgg gtttgaatta
     1501 tgtaatgaaa taaaggaaaa ttacaatata ccaataattt ttttaactgc taatgatatg
     1561 gaatgtgata tgattaaagg ctatgaactt ggagctttgg attatataac aaaaccattt
     1621 aatatcaata tatttaaaca aaaaattaaa gcttttttaa atcatcttac aataaaaaca
     1681 aaacaagatt actataagga tggatatctt gaaattaact tttctgagtt atctgccaat
     1741 ataaatggaa atcaaattat atttacacca ttagaatatc gtactttaaa actgcttact
     1801 gaaaatccta aaagtatttt aacaaggaaa gtacttttag aaaaactctg ggatatagat
     1861 gctaactttg tagatgaaca tactttaaca tctgttataa gtaggattag aagcaaaatt
     1921 gaaaaagaca atcttaagta tatcaaaacg gtatatggta tgggatatat gtggttaggt
     1981 gaaagaaatg aattttaaaa aattttcttt tagttctatt tatttaataa ttgctgttgg
     2041 tataattttt acagctattt tatttagtat ctcaatttat ctaataactg gtgatttaaa
     2101 actaattgct ctattattaa tatatacaat ggtactttgt ggatttatag cattaataat
     2161 atttttactt cataaaaaaa tagttatatt ttgttctaaa atatgtcaca ctttagatag
     2221 tatgatgaat agtgaagata atattgaaat tgaattagaa gaagaaacta ttttatcacg
     2281 tatcaatcac agattaatta gaatgtatga agttattcaa gaaaatcgca atagtattgc
     2341 tatagagaaa gctgatttac aagaactagt ttctgatata tctcatcaaa ttaaaactcc
     2401 tattgcaaat ttaaaaatga ttaatactac tctactaaat caaacattta aaacagaaat
     2461 tcaaaaggat tttttaatgg atatggaaaa ccaattagat aaattagatt ttttaatgca
     2521 atctatggta aaaacctcca gactagagac aggtataatc acactatcta aaaagaaaaa
     2581 ttcaatttat gaaacaattg ctacagcact tagtggaatt ttgtttgatg ctgaaaaaaa
     2641 gaatattgaa gttacagttg attgtgaccc aaatttatat ataattcatg acaagaaatg
     2701 gacttctgaa gctattttta atatattaga taatgctgta aaatacacaa gttctaatgg
     2761 taagatacgt gtagctacag aatgttgggt tatgtataca aaaatagata ttattgataa
     2821 tggaaaagga atatcagaaa gtcgccaagc agaaatcttt aaaagatttt atcgtgaaga
     2881 agatgtccat gacatagaag gtattggaat aggtctctat ctttcaagaa aaattatatc
     2941 tcttcaaggt ggctatatta aagttacatc agatattgga aaagggtcga ctttttctat
     3001 tttcttacct aataaataaa tttttaaaaa tttcatttgt aatgattctg caacattttg
     3061 gttttatact gtatttacaa gatgttaagg aggtattatt atgagtattt tacaagtaat
     3121 taatttaaag aaatattatg gacaaagttc aaacattaca aaagctcttg atggtgtaac
     3181 tctatctatt gaaaaaggcg aatttatttc tattgtaggt acatctggta gtggcaaatc
     3241 cacattatta aatatgatgg gaggattaga taatcctaca tctggaaagg ttattataaa
     3301 gggtaaggaa ttatctacta tgtctgatga acagcttaca atctttagac gcagaaatat
     3361 aggttttata tttcaaaatt ataatcttgt tcctttactt aatgtatatg aaaatattat
     3421 cttaccaata ggattggatg gagagaccat agataaggat tttataaatg aaattataaa
     3481 tatgctttct cttgaagaaa aattatatga tataccaagt aatctttctg gtggtcaaca
     3541 gcaacgtgtt gctatagcta gagcacttgt aacaaaacca gctattatac tagctgatga
     3601 accaacaggt aaccttgata gtagaactag ttcagatgta ctgagtttac ttaaagtgac
     3661 aagtaaaaaa ttcaaccaaa caattgtaat gattacacac aataatgaaa ttgcacaact
     3721 aactgataga attattcgta ttgaagatgg aaaaattgtt aactaggagg tgtagtatat
     3781 gtttagaaat aataatcgca catttataag aaaaatagct ttaaatgatt taaaaattaa
     3841 taaacttaaa acatatcttt caggtatcat tataatgatt tctacatgtt tattattaac
     3901 agtgacctta gtttcatata atgcctctgt agatttagtt aatgcatctc catatcatgc
     3961 tatatataaa tctgtagatg aaaaagcaaa aaatatacta tataaaaata aaaactttag
     4021 tggcgtagga acttacaaat taattggaag taataaaaag acagattgta ttatgtcgat
     4081 tgtttatgca gatgatacag caattaaact tatgaatttt caaccattga aaggcaaact
     4141 acctacaaat aaaaatgaaa ttgctatttc agaaaaatat ttacaggaat ttggtttaga
     4201 taaagatatt ggagatagca ttaaattaaa ttattatgat gatattacga ataaagaagt
     4261 tcaatgtgat tttattattg ttggatttct agaaaattat tatcaagata atgccaagca
     4321 atactataca gtcgtttcaa atgattattt taagaatata gcttctacac ctttacaaaa
     4381 taaaagtaat acttttgacg agtcaagacc tgatactgta gatgtattag ttaaactatc
     4441 agaagaaaat acagagaaag attcagcttc aattaagaca cagttaaata aaattggttt
     4501 atctttaggt attaagaaat ataatatata cttaaatgat aactatattg aatcaaattt
     4561 aattgatggt gagcagatta ttacagtttt agtagttgga atagttgttc ttttttctag
     4621 cgtttttgtt atatatagta ttttttatat ttcagttgtt aattcagttc agatgtatgc
     4681 taagttgaaa tcgttaggta tgacttcttt tcagttaaaa aaaattattt ctctacaagg
     4741 aaatattttg tcaattattt ttattccatt aggggttata gcttcttgta taattgctta
     4801 cataattcaa ccattagcat ggcaaatgat agctgattta tttataattt taattttatc
     4861 actggtaatg tttttaactg ttagaatttc tttatttaaa ccagctagaa ttatttctaa
     4921 aatatctgct attgaagcca tgcaatatac agaaactaaa tttagaaaaa aagcaaaaaa
     4981 ttttagttat ataaatataa aaaatctagc attaaagaat atcgagtcaa atagaaagaa
     5041 aaatttgatt gcattaatat cgctaagcat tagtggagtt ttatttattt caatcgctaa
     5101 tcttgctaat tcaatagatt tcaaaaaaca actatctcaa caatttgtct ataacgaaga
     5161 ttatataatt agcattaaga gtgataactt atatgagcat attacagaaa tccaacaaaa
     5221 taatccacta acagattctc ttaagaatga aattaaatct attcatggag taaaaaaatt
     5281 aattgaatcc aaatcaatta aaagtaatat tatagaacct tacatcaaag atagagacga
     5341 aaaacaattt attactctaa ttaaaggtat tacgcctgaa ctttcaaaat cacttgaaga
     5401 atatattgtc agtggaaaaa tagactatac acatttagat tcagatagtt taattataaa
     5461 taaatataga acaaaatatt atggattaaa tttaaaagtt ggagatacag ttacatttaa
     5521 tatctttagt ggaaatgata ttattaaaaa gaaaatgaaa attataggaa tcattgacaa
     5581 ttccaataca ggaggtatgt tttttgcttc agacaaaact ttagaatctc taacaccata
     5641 taatacaaat ttagattttt ctattttaat agataaaaag cattctaaag aagttgaaga
     5701 aaagttgtta agtataattc agaaaaattc aaatttaatg ctttattcat atgaagatga
     5761 ttatagaatg attactagag catttcaata tataattata gcttcataca tttttgttgg
     5821 tttaatttct tgctttggta tactaaatat gaccaatacc ctaataaaca gcgttttaat
     5881 tagaaaaaaa gagtttgcat tacttcaagc cgttggaatg acaagaaagc aactgcgaaa
     5941 tatgctttat agagaaggtc taaacataag tatcaaagct atatgtactt caagtacact
     6001 aggttatttt ggttcaaatc tactttgcac ttttataaaa gatgtaatta gattagactt
     6061 tattaatttt aaattttcta tatttactat tttaattttt tcattcgtat taattggtat
     6121 acaagtttta gtgactgaat tacttgtaag aaatatagaa aaaaaatcac ttacagaacg
     6181 cttacgttct cagtaagaat gtatataaaa aatagccatt ttttatacaa gttttaatat
     6241 gattttttaa aagctatagg gatagaaaat tttgacttta cataattttc tatccctata
     6301 cttgttttta ttttatacag atacacttac ttatatctcc gagcaacatc accagagaca
     6361 taaacaagca tattccttat tttctaaata tgtcaattgc atgacttgta acccctacta
     6421 aatcttcacg ttcacaagta gcaaggtgat attttaaata taatgcaaat gcatcatcat
     6481 ccatagagtc tactgcttca cgcatatata aagccaaacc atctgatgcg acataatgca
     6541 gtctagttac attaaatgca gacatcaaat catcaatgtt ttctttacgt acaagctcaa
     6601 acaaatcctt tggttcagac ttagcagcaa atgtctgtgg atcaatcaaa cctttttcaa
     6661 tatattcaga tacattaata tttcctctat ggaacccctc gtcaatgaga cagccatcag
     6721 atataacata tgcagcaaaa accacaccac caggttttgt tacacgaatt gcctcatgta
     6781 aagcttgttg tttatcttct ttgttataaa gatgatatag tggtcctaac agtaacgtaa
     6841 tatcatattt attatctggg aaaacagata aatccatagc attaccctga gtaatgctta
     6901 tattttcatt tgattgagta tttttatgga aaacctctat attatgttcg actaattcca
     6961 ctgcgtcaac atcatatccc tgacgtgcca gtgtgtgaga ataacgacca gttgcagcgc
     7021 caatttcaag cacacaatca cctgatttta tgtacttatc aatgtaatgc atagtagtga
     7081 gaaattccac cattccatgt tttaatgcaa gtcgactatc ttcatcataa gtattataaa
     7141 agtcaattaa gtattgtttt gtttccataa tttcacctct tctatattag ttaataaaca
     7201 tcaaatagac aaattccaat ctggtttttc ttgtcttata aaagctgtta atatattttt
     7261 caacattttt tcagcactat atctatctaa tgttaatttt tttgttgctg ttaagacagc
     7321 tatgccatga gtataaagaa ataaatctaa aaatattact tttgcacatt ccaactctat
     7381 tccaatgctt tccgaaaaaa gtcttgcctt tttttcattg tcaatttctt tataaaaatc
     7441 atttgcctct tccatcatca aatctaaatc atttataaat aacaacttaa ataaatgagt
     7501 ttcttcttga gcaaattcaa tgtatgaaag cggaagcagt aaataaggac ttacactttt
     7561 agaattacta taattaacaa catactgatt atagtattca tatgcaaaag caagaaattc
     7621 attttttaat tcattcatgt tttcgtaaca agtaaaaatt ggtcgagtgg aacactgtaa
     7681 tttacttgca atactcctcg catttacagc ttcaaatccc acttctcttg taatatccaa
     7741 gactgtattc aaaatcattt cttttgttat ctttacttta ggtggcatat atgttcacct
     7801 ctccattaaa tttttataaa acacatatgt tatataacta tagtaacata tcaagaaatt
     7861 atgtcaagaa aaagctttgc atttttttgc aattcctaaa acttataaat aaaacatgag
     7921 ttgcaattaa tctattaata gataacagca actcagattt tcctttaaat actcttcttt
     7981 ttgtctaata tttcatatac acaatcttct attgaattaa tatctttttc ttttcttttt
     8041 ttagaatcaa ttaaaatgaa atacagtata aaacctacag caagtcccca tgttccgcct
     8101 ttagcagcaa ttacacctcc cataagtcca atcaatccct tctcaacacc tgtattacat
     8161 agctccattg atagctttgt acataaatat ccttgaacta aaagtgttac tgacaatgct
     8221 aatggtataa gaggtgtagc aatctgcgca attggataaa aacagataaa aattgctgaa
     8281 acccatacta atgtacccat gccactaaaa acagactgca tggcttcctt tccaaccttg
     8341 tacctttgag caacacttcc tgtaagtgtc gcagctaatg gaccgcacat tggtatatat
     8401 ggagaaaaga ttgccataaa aatatttctt atcccactta taacattaga cctatttgaa
     8461 ttgaaatcta tatattcatc ttttctattt tcactttcag aaagtactaa gttttcacct
     8521 gttataaaat caccaaaggc aattatgtat atcataaaag ccattggtat cccttgtata
     8581 aataacatta cacttggaaa acctattcca aaaggagata ctgtatttat cagattttta
     8641 aaatctacta tgtaaactaa attgtcaaaa ctaaatctag gaatttctat ttctttacta
     8701 aatataccta ctattaaact cactaaaata gctgatacta caccatattc tcctaaatga
     8761 aataaaaatt tattcttaac ttttagagta tcaaatcttt cagaaaacat tacaaaacaa
     8821 gcaaccaaaa ttcctactgt tatagatata ggataagaat caaatctccc tttaggatta
     8881 aactctgata ttatagtcga tataccgaca cccattagta ttccaccttt aatagaattt
     8941 ggtatcttac taactaactt tccagctaca ccagttatac ccattaaaat aaatattatt
     9001 cctaaatcta tttgtaaagc tgttaatgct tgtattcttt caatgcccat gctgaacttt
     9061 aacaaatatg cagttgtaag agttatagaa gctgtaatcc agccaggtac aactgggtct
     9121 cccataaaaa catgcaaaca gtataatatt gagttaatga tagccatact ccatgccact
     9181 tcatagggta atcccaaaat ttgtgtacta aggccaattg tgcctaaact tgtcatactt
     9241 gatattatac cttgaatagc ttctggtttc tcaaatctgt aatgtataaa tggaattctt
     9301 atcttaaaca ttccaaattt tatatgaggt tgttcccttc tagcgagtct ttccataact
     9361 tcactccacc tttcaataac tcttataagt ttacaacttt ataattgttt ataaattatc
     9421 aatgtcatta aaattagtat ataacatgca aattatataa acaataaatt aaatctataa
     9481 aattgataga ttttgtgcat atatagaaag accgcttcat ttttagttac ttaaatcact
     9541 taatattagt tatttaaatc acttgataaa ggttttttat aaaattcaat agttctattc
     9601 catgattcaa actgtccttt tgaatttcct tctacagttc cacctgaaga aaaaatccct
     9661 ctactcatac tcatacataa agtctccttt agaggcataa tatatggaat tggtaacgga
     9721 taacccatat tattgtatgt taataattct acgttatatg gataattatt ttcttcaaag
     9781 atttctaatg cctctaaaca agcttcataa ctattccata tgttatcttc ttttccagca
     9841 atcataagta tatctgcttt ggaattttct atttttattc tagcctcttt tctattctct
     9901 gctcttttaa gacttttctt ataagtacta ttaaatccaa atggaatatc ttttgatata
     9961 ttttttttct gttcttcaaa gaatatatca ttgtctactt tgatatatgg aaatggctct
    10021 cctttatatg accatgatga agtatcattt ccactcatca atccattaag agcttggaaa
    10081 caataaatgt gaggttgtga tactacaacc tttttaattt gttcatatct tgaagcaagt
    10141 aaaaaggcca attctccacc ctttgatgtc ccatgtacaa atatttcttt agtactggta
    10201 atttcatttt cactaatcca ctcaaatact ttttcaaaat attctaatgg aacctcttct
    10261 aatttatctg gtaacccttc taaagcaaag tatgcaactg ttagtacatt gaatcctcta
    10321 gatgcaagtg gagctgctaa aaggtctaga gcgtctaaat ttccatcaga accacctagc
    10381 attaaaattg ttttattatt agaattttgt ttataaaata attttcctat aaactcatca
    10441 gatatgtatt ttgtggtaat atcttctgat ttaaatattc tctttaattc aacttctttt
    10501 ctttcaatag aagtttcaaa tatcatattc ataattattg gtttatcaat agatatattt
    10561 tctgcaaaat ttttaccttc agttttagat ttttccagcg aataaattaa ccccatacaa
    10621 ttatcagcct tataagtacc ttctataggt tcaactaaat ctaaatctac ttctcctttt
    10681 tcatttgaac aaaacactcc ataagatgaa aattcttctc ccttacacca tggaaattcc
    10741 attttcacac ttattttaag tttagtattt ggaattaagt ctgaaaccat aatcttaagt
    10801 ctttcatcca caaatgattc tacattgtat acatttactt tcataatcta tttctcctta
    10861 taataattat ttatgtttta tataaaacag ctttatctaa tacaagataa ataaattttt
    10921 aaattaagac accatattga ctatttcatt ttatcaacat atttcatctt tgtcaacata
    10981 ttgtcttttt tagtaattta aaattatggt ctatatgaaa tccagttgat ttttggctaa
    11041 ttaaagatag gaatgctatt aggtttagaa tatatatatt caaaataaaa aaccactcat
    11101 ttaaagaatg gtttttaata taaactattt gttattaata aggatatttt tttatttaaa
    11161 tttatataat cgaacatcta atatttaatg tttaataaaa ttatgcattt gcaaaagtat
    11221 atatggcatt aactcttgct acatataaat tctcatcact acatccaata tatctagcat
    11281 atttttcaat cttttctttt gcattttcaa catcattttc atcgctctcc attaactcat
    11341 ttatataaat atccattaat gacgctttat attctcttat atcatagcct aaaaattcag
    11401 tcaatctctc aatttcctta ctaaaatctc catcttcttt taccaaaaat tcgtatacat
    11461 cttccctata tggatttaaa gttattattt gttttactac tttgcattcg tcagccttcc
    11521 caataaaacc atctttataa ttattaaata aggcattaga tttttttata gattcttcat
    11581 caatataaga atgtaccgca gttatacgat tttctataag ggcatctaca acagcataat
    11641 gaattttaaa acaatcattg tataaataat tgctcatttt ctttattgtc gattctattt
    11701 ctaagaagct cgcgtggcca ttttttttat ttttatttgg tataataaaa gttttattca
    11761 aattattaaa aagtctctta aaatctaaat atttttcaca ataaacactt ttaaaggtat
    11821 cgatatctat agttataatt ccaaaactta ctatagtctc aattcctttc ttaaaagact
    11881 cttctatata tttttctcct aaacttaaac ctctatcaga tatttgcttt agattttgaa
    11941 aactatttaa acaatctctt tcaaagtttt tagatatttc taaagcatat aatttaaaga
    12001 ttttccttat cttattataa ttattttttg tatcatcaaa tctgataata gctccattta
    12061 catcaaaagt aatcattgag ttttaacctc cccattagtt tcatcaataa aataaattta
    12121 atataaactt tataatcata atattaaact tatttatact ataattatta atttgatgat
    12181 aaaagctatt ttccttctaa ataaaaaatt tgtctaaagt ttctaaaaaa tttagacaaa
    12241 tcttaggtta ttactcttta aattattttt aaattattcc aaatacgcca aataatattg
    12301 gtataaatat agctggtaac aaattggcta cttttatatt cgttaattta agtacattta
    12361 gaccaagtcc tataattaat aaacttccag ctgctgtcat ttctgtgact acaggatcac
    12421 ttaaaaatcc atttaaaaat gatgctccca cacatataat accttcatac aaaaatacag
    12481 atattgcaga aaacattaca cctataccta aagaagcagt aaatataatt gaagctattc
    12541 catctataac agattttaca aataaagtat catgacttcc tgtaagacca ctttctaatg
    12601 aaccaactac tgccatggct cctatacaga ataataaact agatgatata aaaccctctg
    12661 ctatagaatc ttttttattt cctttagaaa atttactttg taaaaaagca cccaacttat
    12721 ttacccattt gtcaatatct attaattctc ctatcagagc acctacagct acggatatta
    12781 ttgtcactaa tgtgtttttt ccttctagtg ctcctgatat accaatgtac aatacacata
    12841 aagcaattcc attcattata gtatcgctta ctctttgagg taatcctcct tttattataa
    12901 gacctactat acatccacct gcaattgcta gactatttgc tattactcca aacatttttt
    12961 ctcccctcga ttagctaatt gaaatttatc ctattattaa aacctaaaca tctatacttt
    13021 gtattggata ttttaaatat tccaaaaatt cttcaaaacc ttttctagat atatttacag
    13081 ttttagtatt tatgttagga tgaaaattta agtaatcttc tttaattaca tctttatcta
    13141 tgtaaagctc aacttttctt tctgtatcat ttattaatga aaaaggattt acacttccag
    13201 gaagaagttt aagtacattg tatagtcttt caggtgaagc aaacgataat ctagaacttc
    13261 ctatcttatc tttaagtgag tttaaatcaa cttgtttttc tcctttaact acaataagat
    13321 aatacttatt tcctttggca tttcttaaaa ataaattttt acattgacac ccctttgtta
    13381 ttccatctaa aatgactaat tgctctgctg tatatacagc ttcatgctct actacatcat
    13441 attcaacatt taatttatcc aaaatatcat atattttagt ttcattcatg ctagattcat
    13501 tttcatcttg ttctttttta ttatttaaat cactcatact tttctcctta aacacttcat
    13561 cataatatca ttgtgaggta ttattctact atagctattg ctcttactgg agctccatca
    13621 gcatcattat acttaaatgg tgctgctata aatagaaatt tttctggaac attttctaaa
    13681 tttgtaagat tttctatgat tatttttcct ttttcaaata aaatatgatg cacttcaaat
    13741 actgatgtgt catatctatc cactgatagc atatctattc ctatgccttt tatattagta
    13801 ttagctatat agtttgcagc ttcttttgtt aatgtaggat aacctacata gtactgcttt
    13861 ttatcccaaa attttgacca acctgattta aatattataa aatcactatt ttttatttca
    13921 tcttcatatt tttttagata ttctaattct atattctcat ttcccttttc aagttccaat
    13981 acataagctt ttccaacaaa attttctata tccaacttat ctagggtctc acctttagta
    14041 tacatatgtc ttggtgaatc catatgagtt ccattgtgag aaaatacact aattaatgtt
    14101 tcttgataac cattttcttc aataatagct actttcttga tatctggtct attaggctct
    14161 gcatatacag gcatgtcatt atgtgtcaca tgagttaaat caaaaacctt catctttata
    14221 taccccctat gtttttattt tctattatat acctaaattc attttatttc tatcagattt
    14281 tattccatta tataccatac tactatatat tttttataaa cctcaaggga tttaccgaaa
    14341 aatcttggaa agaattatca gtaaatataa catcttcacc caaaaaacgt ttataccttc
    14401 cttcttcttt ctttaaagta attctctgtt tttgtgaatg attattaaaa accattatct
    14461 taagaggagg tttttttcta ttagtctgca ctctttttaa catgtatttt atgtgcatat
    14521 cagcttcagg aaatgaatat ccacaaaata ttaaaaggtc agtatctctt aaagtttttt
    14581 ctgtttcatt ccaaacatta cttaaaaata tgtttgacat attcttaaaa tatgttggtg
    14641 gcactataat aggttctgtt aactcaccac actctaaaca ttttgtctct gaactatttt
    14701 caataagctt catgacacca ccttcatgtg gtgtaagagt tacactatta caaatggggc
    14761 aataaagcca attcaatgac ccatgtatct tatataattt gattaatggt tcttttggtt
    14821 ttctccaatt taactcctct ttaaaatttg taaactccac accataatca agcataattg
    14881 gcaaatcatt cttatctctt aatccagtaa ctgtattgtc tatatgtata tcataatttg
    14941 cactaataaa agtagtatca agaagcaaat cattttccaa aagattttca actaaaagtt
    15001 tatgatattt attatctgtt ctaggtgtat tatgaattgc ttcagccatt agcagtatta
    15061 aatattgtct taaaaaacgt atactatcac ttctgttatt tagattttct agtccaaaat
    15121 ttctaaatgc ttcttttctt tgttctgcta agtctaaaag acctaaaact tcttcaaagg
    15181 taggaaaatt tgcattatct atattatctt ttactatatc aatattgaac atctgtttaa
    15241 agaaaatata caattctcta ttcattttgg tattataatt tgtgggattt agttttttaa
    15301 aatattcgct aaaaagttca ttttgaatag gtagattctc tgctgctgaa gctcctgctc
    15361 ctaaaaatat tgctattttc atatagttcc tcctaatata aatattactc ctatttttat
    15421 atccattaaa tatccatatc atttatttta tgatacaaca ataaagctgt cttttactag
    15481 aaaaaatcct attacaagac agccttatat tatattaaat ctttaataat tcgtaaatag
    15541 aaagagcata tatttgagta gcttttataa tatcctctac tattaagtat tcattagctt
    15601 ggtgttctgt ttctggtttt cctggaaaaa tagctccaaa tgcaacacaa ttatctaaag
    15661 ctcttgcgta tgttgctcct ccagatgaaa gtggtgttcc atcaagacca gtttcttctt
    15721 catatacttt tctaagtgtc ttaacaagaa gagtatcttc tggaacatag attgaatcca
    15781 agaaatcata ttcttcataa tttaaattgt atttatctgt cattttcttt aactctttta
    15841 caaaatcatc ctttttgtat gttactggaa tccttacatc tattcctgca aattcttttt
    15901 cgttatcaat agttactcta cctatattta ctgttagttt acctgataca tcatccttgc
    15961 aattaggaat tatattgttt ccattagcat cttcaccaat aacttctgct aaaaacttaa
    16021 ttatattaga atctatacct atattattaa gagctataca aagtctagcc actgcattta
    16081 taccagtatc acttgctgct gaatggacac tttttccaat tatacagatt ttattacctt
    16141 caacagtata ctcaaaattt aatttatcaa gttctttttt taatttatca gagtattttc
    16201 caatatagat tgctttacct ggaactgcat ttaaagcctt tccaactgac aactctatat
    16261 cacttttgct atcacaagtc aaatgaactt gtaaaagacc tttctctgca tttgttattg
    16321 ggaatctaga atctggagta aatccatagt ttggtatttc ttcattattt tctttatatt
    16381 tatttataca tctccaaaga ttttcttcat cagccccaaa aatgaatctt atcctcttat
    16441 taaagtctac atttaaatcc ataagagctt tcactgcata aattgctgat attgtaggtc
    16501 ctttatcatc ttgtgttcct cttccataca atttaccatc ttctaaaaca gcttcaaatg
    16561 gagggtaatt ccagctttct aaatcacctt ctggaaccac atcaacgtga ccaagtatac
    16621 ctatcatttt ttcaccttgt cctatttctg catatccata atatccatct ttgtatactg
    16681 tcttaaatcc taaagattta cataagtcta atatacctct taaagcttta tctacatttt
    16741 caccaaatgg gcaattttca gttgcttcag atataacact aggtattttt acactttctt
    16801 gtattgaaga aatcatttca tcttgtagag aatttacttt ttctttaatt tgctctttca
    16861 tattatcacc cttcaatttt catcataata tactaataaa tattttgacc ttttaaaaaa
    16921 aaaatatgct tattttttca gtttttttat aaattcaatc gcttttaaag tccccatttt
    16981 tgaatggtca aatgtaagtc caccttgaaa ataaacatta taaggaggtc ttattggagc
    17041 atctgcactt aattctatag aagaaccttg tataaatgct cctgctgcca ttatgacttc
    17101 actttcgtat ccaggcatgt cccaaggaac aggttttaca taagaatcga ctggtgcagc
    17161 ttcttgtata ccttcacaaa aacttattac ttcatcagca ttattaagtc taatacattg
    17221 aataatatca cttcttaagt catcatattt tggtaataca tcatatccta atttttcaaa
    17281 agctcttgag caaaagatag ctcccattac agcctgagat actatataag gcgccaagaa
    17341 aaaaccctga agtacatttc tagttgttcc aaaagtaaga ccacattctt ttcctattcc
    17401 aggagaagtc attctataag atataagctc aatcaaatct tttctaccag ctatatatcc
    17461 acctgtcaat gcaagcccac caccaggatt ttttattaaa gagccagcca taacatctgc
    17521 tccaacatca gtaggctctt tggtatctaa aaactcacca taacaattat caaccattac
    17581 tatagcttct ggtttaacag actttattac ttctatagct tcttttatat cactaattga
    17641 aagtgatttt ctccaagaat atccttttga cctttgtatc ataacgagtt tagttctatc
    17701 atttattttg tttttaattc cttctaaatc taagtttcca tcctctaaaa aatctacatc
    17761 atcatatgta acaccatact ctttcaatga acctttttct tctcttatgc ctataactcc
    17821 ctctaaagta tcatatggtc taccagtaac agataaaatt tcatctcctg gtctaactat
    17881 accttgtata cataaagtaa gtgcatgtgt accatttaca attattggtc ttacaagagc
    17941 atcttctgta ttaaatactt tagaataaat ttcctctatt ttttcacgac caatatcatt
    18001 atatccatat cctgtagtcc agttaaagtg catatcactt agtttagatt cttgcatagc
    18061 ttttaaaact ttatattgat tatattctct tatttctttt atttcttcaa acttatcttt
    18121 tatttcttcc attatttctt gcgatagttt aaaagtatca tcatctattc catagtagtc
    18181 ttttaataat tcttttgtct cattaagcat tttatcacct gtctctttat tattttattt
    18241 aaacatgtaa aggagactat tttactaaaa caaataaatt atagtttata agttagtata
    18301 agtctcctct caatcatctt aaattaaatt aattatctat ctgttgttat tattattgtt
    18361 gttttggttt tgatttgtta gattaatata ctttccaggt tgtatagttg atacagcatg
    18421 tttataaatc atgttttgtt gtctattatc cccttctatt aatattatat aactatcaaa
    18481 cccctttaca agccctttaa cttgcactcc attaactaaa tatatagtaa caggtatcct
    18541 ttctttcctt gcattattta aaaacaaatc ttgtaaattt aaaactgtat ttttcattag
    18601 atttgtaccc tcccaagatt aaattgaact tctaaaatat tcctacctta tattctatat
    18661 aagttatgac aaattttcaa tattaagttg tatacttaaa aatttatata ttattttata
    18721 gaatctttaa tatataagat tatttcattt tttaattcgt ctatattttc atattggtct
    18781 aaatcaaacc attttgctgt tttatatctt ttaaaccatg ttatctgacg ttttgcatac
    18841 cttctagaat ctcttttaat tatttcaata gcttcttcgt atgtatattc tccatccaga
    18901 tactttaata tctctttata acctatacct tgcattgata tcatatcttt tttaaatccc
    18961 atacttaaaa gtttttttgc ctcttcaacc aatccatttt taatcataat atcaactctc
    19021 atatttattc tttgatacaa atgttctcta tctctattta gaactattat tattggttga
    19081 tattcttcat taaattttaa atcacttgaa aaatcattca ttttcttacc acttagacaa
    19141 acttcaagag ctcttatgac ccttttagta ttgtttttat ggattctatt agcagattct
    19201 tcatcaagtt cttttaactt attatgcatg taatctattc ccttttcagc aagctgtttt
    19261 tgaagttctt ctcttaattc attattggca tcagattgtg caaaatccat attatatatt
    19321 aaagagttta aataaagtcc tgtgcctcca gttactaaaa cacatttttc cttttcattt
    19381 atttcatgta tatatttttt tgctctttgt tgaaactctg aaactgaaaa tggatagtca
    19441 ggcttaactt catctataag gtaatgcttc actccttgca tctcttcctc tgtaactttt
    19501 gcacttccaa tatccatata ttcatatatt tgcatagaat cagctgaaat tatctctgca
    19561 ttcatatctt ttgctaattt tattgacaaa tctgtttttc caacagcagt tggacctgta
    19621 agtattataa gtggtatttt tttcataaca ttcctttcca tatattttaa atatccaaat
    19681 attttacata attcttttaa acattttttc tatttctgtc ttagatattt cgaccattat
    19741 aggtcttcca tgaggacatg taaatggatt ctcacacttt tctagctgtt ccaataaact
    19801 ttttatttca atatcgtata ttttatcatt tgcttttata gcagacctac atgccataga
    19861 tgcaaatctc tctcctttta aatcataatt acttgtaatt tcttctatat tatcaattat
    19921 ttgaagtata aatttctcag tttcaggtac tccaaatata gtaggaacac atcttaccat
    19981 tatatgatta ttgccaaaaa tttctagctc aaaaccaaat ttcataaata actccaagtt
    20041 attttcaatt tgaagcatat ctatatttga tacttcaata accactgggt ctaaaagtat
    20101 ttgcatattt atatcttgtc tatagaactt ctccatatat ctttcatata gtattctttc
    20161 atgtgcagca tgttggtcca acaaatacat agaatcgtct ttgctcaaaa ttatataagt
    20221 attaaataca actccaatta caaaatatcc gtataaagaa aacttgcgtt ttgaactaca
    20281 caagtattct tcttcactgt ccttaatact atctcctaag taataatttc cttcattttt
    20341 aattccatca acttgaaact cttcttgtat attatcattt gcactatctt cacttagata
    20401 attaatgttt tttacatcat cttctacact tgcatttagg acttcactta ctgattgaaa
    20461 ttcatttatt ggtttttcac ttgataaagt aacaacttca atgacttcat ccttaccttt
    20521 agtaatatta ctatttttag gagtagattc taataaatca ttatttctta atttataatc
    20581 tgtacttttt tctctactgt taatagcgat tctaggttgt gtttttttat cactataagt
    20641 agcatatttt ccaataagat ttgagtgtat taatttaacc tttaaaaaat ctctaagttc
    20701 tatatacact tcttgttctt tttcaaattt aatttcaagt ttattggggt gtatatttac
    20761 atctatacat gatgggtcta cttctatatt taagaagcac actgcatgtt tacctattgg
    20821 aattatagac ttataacttt ctgttattgc atctatgatt attttacttt ttacaaatct
    20881 tttatttata tagatatgtt gtaagttttt atttgaacgg tatatattgt tattacctat
    20941 atatccattc attttaaagt gattgcactt aaactctaca tctattatat tttccgttat
    21001 ttcttttcca tatatacttc taatagtgtt tacaagtttt ccatcccctg gagtatttag
    21061 catctgttta ttgttatttg tatatttaaa ttgaatgttt ggattaccta ttgcaagttt
    21121 atttatcaaa tcacttatgt ttatagtttc tgcatgagta gattttaaaa atttctgtct
    21181 tgctggtgta ttaaagaaaa tatcttttat aatgatggta gttccattag tagaacctat
    21241 tggttctttc gatattattt ttccaccttc aacatatatt ttagttccta ttatttcatc
    21301 tttagtttta gtcgtcattt ctaattttga tacagctgaa atagatgcca atgcttctcc
    21361 tctaaaacca agagagtata aatcatacaa atcatcaatt tttttaattt tactagtagc
    21421 atgtctcaaa aatgattttt caacttcact tgaaggtatt cctattccat tatctgtaat
    21481 cttaattaga ctttttcctc catcaattat atctatggaa attttatttg cacctgcatc
    21541 aatggagttt tcaataagtt cttttactac agaagaaggt ctttctacaa cttcacctgc
    21601 tgctatctta tttatagtca aatcatctag gatgttaatt atatttttca tttttacacc
    21661 tctaagattt tatttctttt gcttttcttt gaagattata aagagagttt atagcatcta
    21721 atggagtcat atttaaaata tccagattta aaatttcttc tgatagtata tctctattta
    21781 cactatcaaa agagatttga gttaaggcta cttcttttac agaatcttct tgattaattt
    21841 tctctttatc atcattttgc ttcattaaag caacttcatc atttaatgct ttaaatttag
    21901 aatttaattt atcataatcc ttttttatat gtttgtagtc ttttactata agttcatgtt
    21961 ctatttttag agattcatat ttattcttaa aattactttg atttacctta actaattctt
    22021 cgtccaaatt actattaatt atattattgt tttctttatc tccatttatt gcaacactgt
    22081 tgtaaacatg atttttttcc aagtctttaa gtatatattt tgctctttct atgacttcgt
    22141 ctggtaattt agctaattta gcaacatata ttccataact cttgtctgct ccctgaggaa
    22201 ttatttttct taaaaatatt attccttccc catcttcttt gactgctatt gaataatttt
    22261 taacctcttt aaactcttct tctaaatcag taagttcatg ataatgtgtt gcaaataatg
    22321 tcttacatct aatatttttt tgaatatatt ccactattga ccatgctaaa cttataccat
    22381 cataggtact agttccacga cctatttcat ctaatataac taaacttctc tctgttgcat
    22441 tttttaatat aagggatact tcgttcatct ctaccataaa agtagattga ccttgagata
    22501 aatcgtcact agctccaact cttgtaaata ttctatctaa tattggaata tcagcagact
    22561 ctgctggtac aaatgagcct atatgtgcca taagagctat tatagcagtt tgcctcatat
    22621 atgttgattt acctgacata ttaggaccag ttattatgtt aataatgttt tctccacgat
    22681 ttaaataagt atcatttgga acgaaatttt cctctcctac tatattttcg acaacaggat
    22741 gtctaccatt tcttatatca agtttattat tttcatttat tgctggtttt acgtagttat
    22801 ttatatgtgc aactgttgca agggacacaa aaacatctat attagctata gtcttagcca
    22861 ctttttgtat tctatctatg tttttatata ttgtatctct tatctctaca aatatctcgt
    22921 attctaaaga tttaatcttt tcctctgcat gtagtatctt ttcttctatt tcctttaatt
    22981 ctggagttat atatctttct gcattactaa gtgtttgctt tcttatatat gtctcatcta
    23041 gctttgcctg tttaaagttg gcttttgtta tttctatata atacccaaaa actttattaa
    23101 atcctatttt taatgattta actcctgttt tttctctttc cctgttttct atttccttta
    23161 caagaaaggc tccattctta gaaatctctc ttagttcctt aagttcatca ctaaaatctg
    23221 atttaattat gtttccatct tttatggtta tcgtaggttc ttctagaata gcttcatcta
    23281 ttaaattgta tatatcatct aatttatcca tttctgaaac atactctttt aggatttttg
    23341 catttgataa atttatagta tcttttaaat taggtaactt ttctatcgaa ttttttaagt
    23401 gaattaactc ttttggtgtt actctctcaa atgctatctt tccacatatt ctttctatat
    23461 cgtatatatt tttaagtata tcatttaggt cttctcttaa tataaagtca tcttttattt
    23521 cttctattac atctaatcta ttttcaattt tagatttatt tatcaaaggt tcttctacat
    23581 attttcttaa aagtctacct cccattgctg tagaagtttt gtcaagtaca tgtaataatg
    23641 aacctttttt cttattccct cttatagttt gagtaagttc taaattagtt ctagtaaaca
    23701 tatctagaac catatattct gatgaattgt atatatttat attattaatg ttagatgtta
    23761 tttgtttttg agtattgtat atataattca gaagaattga taagcttgac tttattagac
    23821 ctttatcatc aaactttagt ttttgtagat aaacatctgg aaaatattct tttaatatat
    23881 ttatatccaa atagttatca ctaaaacttt catttatgta tatattactt acagtagcta
    23941 tatctctaag cttttctata aagtctaaat catttataat tatttcagtt ggatggattt
    24001 ttgcaatttc ttcaattacc ttatcttcat ttaaacatgt tgcattagtt tctcctgtac
    24061 ttatatcaac ataagttaga cctatattag taccatcctt atacaaagat aataaataat
    24121 tattcttttt attttcaagt aaatttccat ctaaaactgt accaggagtg atgaccctta
    24181 taacctctct tctaacaatt ccttttgctg tagatgggtc ttccatttgt tcacctattg
    24241 ctactttata tccattttca actaattttg atatatatga attggctgag tggaaaggaa
    24301 caccacacat aggagcacgt tcttctaaac cacaagcttt tcctgtcaat gctatttcaa
    24361 gagcttttga agctactaat gcatcttcaa aaaacatttc ataaaaatct ccaagtctaa
    24421 aaaacagtat acagtcttta tatctatttt ttacttcaag atactgtttc atcataggag
    24481 ttaatttatt catatctatt tgcattatca aacctccttc ttttctaatt aaatattata
    24541 tcatagtaga tgattattat taacacaata tataaaattt tgacatataa ctaaaaacaa
    24601 tccaaaatgt atactcacat tttggattgc taagtttaat gcttattaaa taaaaatatt
    24661 tttttttaca ataaagatat tatttctttt ttaagtttaa tgatattttc aaggttatct
    24721 tcatttaact ttctactatc tataacaaat tctttcttta ctattgatgg tttttttgaa
    24781 ataacataaa ttctgtttga tagcattata gcttcgtcaa tatcatgagt tataagaagt
    24841 attgttgaat ttactttttt cttaacatct aaaagccatt tttgcatgga tgttttagtt
    24901 aatgaatcaa gtgccccaaa aggctcatct aaaagcatta tgtcattaga atttataaaa
    24961 gttctcaaga agttagctct ctgtctcatt cctccagaaa gttctgaagg atatttatct
    25021 tcatatccgc ttaatccaaa cacctcaaaa taaggtttta ccattgacct agctttactc
    25081 tttttttctt tttttaaaat tagtggtagt gacacattat ctattatagt tttatatggc
    25141 aagagtaaat ctttttgata catataacta tacttaccat ttatattcac tttgccacta
    25201 tcataatctg taagtttagt tataatatta aaaatagtac tttttccaca tccacttggc
    25261 cctaatatag aaacaaactc accttcataa acatcaatac ttatatcttt taaaacttca
    25321 atatctttat aagtttttga tacattttct atagatactt ttactttttt attgtggtaa
    25381 aaattcattt gtgtaagcat cacttgcctt catatcctta tctattaatt tatattcttt
    25441 taagaatgat gtatagttgt tccatacact atctttcatt tctccccatc gtggtgcatc
    25501 atctttgtac ttgcttgcta aatattcttg actctttaaa gctagttttt catctacttc
    25561 aggtgcatgt tttactaaaa tttttgctga ttcttctgga ttttttattg catattcata
    25621 accttttgtt gtagctttta agaatttttt agcaagctct ggattatttt taagcacagt
    25681 ttcattagaa attataagtg gtgtataata atccaatctc tcatctaaat cttttactgg
    25741 tataaagttt aagtcatatc caattaaatc agctttaact gcatcccaac cttcaaatat
    25801 ccatgcaaaa tctacagttt tcatagctgc aaagaaatca tcttgaccag tattaactat
    25861 ctttaactta ttaaaatcag ctccattttt cttcataact gctttaaaaa cagcttcttc
    25921 tgatggagag ccccaaccac cataagtttt tccttcaaaa tctttagctg ttgttatatt
    25981 cttttctttt ggtgatgcaa acccagaagt attatgttgt ataacagttg ctacagcttt
    26041 tattggaagt ggatcttcac tagtctttgc ataagtaact tgttcttgat agcttatacc
    26101 aaagtcagct tttcctgttg ctactaaagt ttctgctcca ctttcaggtg gttgtactat
    26161 ttcaacatcc aaaccttctt ctttatagta acccttatca agtgctacaa ataatccagt
    26221 atgatttgta tttggtgtcc aatcaagcac catagttact tttttagaag aagtatcttt
    26281 atcattgttt ttgtctttat tgttatttga agaacatcca gttattgttg ccaatgtaaa
    26341 aacacttact aaacctaata ttcctaactt tttaaaattc ataattgcct ccctcaaata
    26401 cctttaatct attaatctat gtcttataac aacttttttt acagcctgta caattcctac
    26461 aaacattaaa ctaaatataa ctactaatat agtagatgca aatactttat caagtgcata
    26521 tgcacttttt gacctaagca tataaacacc aagtcctttc gttccaccta gccattctgc
    26581 aacagttgct gccattacag catacgttgc agatatttta agtccagaaa aaaatttatc
    26641 cattgccata ggaaatttaa ggtgaatgaa tgtattaact ttatttgagt ccatagtctt
    26701 aaataaattt aaataatctt tatcaatatt ctcaatgcca tctacaaaac ttattaatat
    26761 aggaaaaaaa caagtaagca ttaccatcaa tacttttggc attgttccaa atccaaacca
    26821 tatcataaag agtggtgcta taattattgt aggtatcatc tgtgtaacca acataatagg
    26881 ataaagacat ttttttatta ttgatacaaa atccatcaat attccaatta ccaatgatat
    26941 tataattgcg actataaaac caagtattgc ttctcctata gtaaccacag catgtatgga
    27001 taaattttga taatctttaa aaaacacatt aattatatct actggtgtgg gtagtatata
    27061 ctgaggaact tctaaaactc ctaccataga ttgccaaaga ataagtataa acaaaaatgt
    27121 tgctattgga tatatactat ctttgacttt atctatgatt tctaagtttt tcatctatag
    27181 tcagacctcc agctttttca tctactctag ctactgttat gactctttca catccaagat
    27241 taaaagggat tttatgtacc ttttttaata attcaaaaac ttcatcaaaa tcaccttcaa
    27301 cagtagtgct cattgctcct atttcacatt taagaccact tttttcaatt tcagcaatac
    27361 aagcatctac tactttatac atctcttctt cacctttgta aggtcttagt ggcattactg
    27421 ctatgtctgc gattaccatt taaatcactc ctttaaaaat aattaatacc tactaggtat
    27481 attttgtttt ttattaaaaa attttattac taaaaagata ttatatttaa ataaaagaaa
    27541 agtcttatcc aaaataggat aagacttatt tttatcttaa ctggtctccc tacactggca
    27601 ttatccaaac aggttcaaag ggtcgagata aaatctcctc tcagcacatt agctccccga
    27661 aatatattat atcttttcta cttgattata ctacacttat catattttaa caaatttttt
    27721 atttatatta gatattttat ccgttcaaaa atatctaata taaattgtac attattacac
    27781 caaataaatt atcttaaaat ggtaatttat caacatcttc caaaactaga ttaactgaat
    27841 tttctagttt ttattttgct atttcaagca tatttggatg tatatcaatt ccatacaaat
    27901 attgtgctaa atttctgttt tcactcatct aatttgcaat cgttacaaat tacagattag
    27961 aaatagcttt tagactctac ttatctttat ctacaaaaaa tccttcaata aattcattaa
    28021 taatattcag taatctaaaa ataaacctct atctagattt ttaagataca agccttttag
    28081 gaagtatctt ttgtgataat ataaaaatta ctcatcatta tcatcaaaat cctcagcaac
    28141 caacatttta gataagaatc aaactctata atataattat tttatccaat ttatttttga
    28201 gaaataaaat atttttttaa tttttaaatt ttttaatatg caccttgaaa aaagaaacgt
    28261 tttcctatat tatggtaata gtagaataaa actaaatagg caaaactaga gaaatttagt
    28321 gacgcaaagc tatagggact aagacttatg aaatgagtta tgtcagccag ttgccaaaga
    28381 gatattttct ttttgttttt atgaatgttt tttaggggga tatatgatat atgtttatgg
    28441 ataaaatgga tagatgtaca catatactaa ctgcatatat atgcagttca cgcgattact
    28501 gcaattttat agatacacaa ttaaatgatt ttatactaga atatggagag aatgtggtag
    28561 aatcttgttt gcatcaagtg atggtattgg taagcagata taattaaaga aattttattt
    28621 tctagaaaac cctttttagt tttatctacg ttttataatt cattaaagaa ctaattaaaa
    28681 taagcttcat tatatatttt tcataaagta aacagttgag tttatctcat atactgttta
    28741 ccccaattac ataattctaa taatatagat tctaatgatt taccacgttt agtagacaaa
    28801 tattcaactt ttggaggaat agtcggataa gaaatcctct taataagtcc atctctttca
    28861 agatacttta attcttgtga cagtatctta tgacttatat gaacaacttc tttttttaat
    28921 tgactgtaac gtattggctc ttgttttaaa atatgccata aaataattgc cctccattta
    28981 cttcctatca cagtcatggt aaaaccaatt gaacaattaa aatctttttg tctttcttca
    29041 atatttggaa attcttcaaa attattatct atatccatct caatcctctc tttcaaaata
    29101 gtaagtatct aactaaaaag tgcattcttg tttagcgtac taattcgtaa tataatcttg
    29161 attgtagtat agagccttac ataatactac ttttttagat gtatatgttt aaactttaat
    29221 atagacatat gttattttat taattttatt taaacagata ataataaata ataatttatc
    29281 tcaagttttc tatgtagata aactaatcta atgtttagaa aggattatat aatatgaata
    29341 aaaaatttcg tctttatcaa gttgactcat tacaaaagaa aaatttacag gtaatccagc
    29401 tggtgtaatt acaaatgctg atggcttatc tgaatcacaa atgcaaaaaa tagcaagaga
    29461 attaaataat tcagaaactg cttttatttt caataacttg gctaaaaatc atcaagaaaa
    29521 ttatgatgta catatcagat tttttacact aactaatgag gttcctatat gtggtcatgc
    29581 tactattgct gcccattatg ttagagcagt agaaaaccaa ttcaataaca aaacagtatt
    29641 tcaaaaaact agtgctggaa tattaccagt tgatattata aaaaaagata atgattatga
    29701 aataattatg acacaaggaa aaattgaagt atctccaccg ttacaacaaa cctatcaaaa
    29761 aagattactt aatgcatttg gtattttaaa ggaagatatt cgtacagatt gtcctattgc
    29821 catatcatct acaggacatt caaaagttat gattggaata aaggaattat caacattaaa
    29881 tagtttatct ccaaatttat atgaattatc gaaaataagt caagaaataa aatgtaatgg
    29941 atattatatt tttacattac ataataaaga aactctacaa gttcatggaa gaatgtttgc
    30001 tcctgcaatt ggtatttcag aagaccctgt tacaggaaat gctaatggac ccttaggtgc
    30061 gtaccttgta cattataaag taatacctac acccaacaca gagtttatat ttgaagcagt
    30121 tcaaggagaa acaataaaac gcaaaggcaa gatgttagta aaagtaatta ttttagataa
    30181 taaaccagtt gaagtaaaaa taattggaaa agctgtaatt gcattctcaa caaacataga
    30241 aatctaatta tagattcctt ctaagtaaat ttttgaactt tttaatacta atttaataac
    30301 aaactaattt atatacaatc aaattaattt ttttattatt tgttaatata tatttatcat
    30361 tatattatat aataatatag atagttacta tatcttataa ttagataaat cataattata
    30421 tattattgtt aaaactcaat tatataattt ttaataatat tattttagga ggtaaaaatg
    30481 ataaacgata ttaaaaataa attcaaaaat tacaaaccat atataaatgg atacaagaat
    30541 atgaagagag catctgtttt aattccaata gtagaaataa ataacacaca ctatatatta
    30601 tttgaagttc gctcaaaaaa tttgaagcat caacctagtg aaatttcttt tccaggtggt
    30661 aaaatagaat caggtgagac tccatatgaa gctgttatta gagaaacttg tgaggaactt
    30721 ggaactttta gtgacaatat agaagtaata tcaagtcttg acctccttat aacaccagta
    30781 aagtttatca tacatccata cttaggatat ataaaaaata ttaataattt aaatataaac
    30841 aaggatgaag ttgaccatac ttttttggtt cctataaaat atcttttaga gaatcctcca
    30901 aatacttata gtaatactgt aaaagttttt ccagacgaaa atctaccata tggttcaatt
    30961 ccaaaccaaa aagattataa atttgaaagt ggaagttatc ctattctgtt ttataaatat
    31021 aaagattatg taatttgggg aatgacagca aaaatactag aaaacttttt aaatttttta
    31081 tcagaataac actcataata aatcatcaaa acttttacat aagaaattta taaaagtttc
    31141 taaatttaac aattctaaaa tatcaataac tatattttgt agcacttttt tattttatta
    31201 ttgtttgaat atttgatggt tttcaatgca atttcaacat aaatactatg tattagtgta
    31261 tactgttgat gaattaaagt atgataatct atttaataaa gtatgaagta aatgatttta
    31321 atgtgccttt atggtaaaat tactaataag taataaaatt aattaaaata tatggaggtg
    31381 cttataatgt ttgctgtagg atttgttgtt ataattgcac ttgccttttt cattttaagt
    31441 gtactattat ttaatggaaa agccagtatg ctactagcag gttataactc tatgagtaag
    31501 aaagaaaaag cacgatacga taaaaaaaaa ttatgtagaa gttctagcat tgttactttt
    31561 attgtctcta taatgctatt tattatggcg tttttgggat atagaataga gagtggtcaa
    31621 atggacgaaa aaaaaatggt tcccttttct ataatattta taatagtcat actttccagt
    31681 gtatttttta atatcctcta ctctaacaaa aaatgtaaaa aataaatagt tgtaagttga
    31741 gcagttaatt ataaaagatt gactgctttt tcatgtatat tgactactct tattaaatat
    31801 tttaacattt aaaaatctat gtaaaaataa tgaatatagt acaaacttct aataatgatt
    31861 ttacctttgt ggttacagtc cactatatat aatttaatat gataacaggg aattactaat
    31921 gacaactagg taagctttta taaaatataa taaaacaaaa atagatgtac aatgaaatta
    31981 tatcttatat caaatttatt tttattaata gcaaaaactt acattagcat aacatatgac
    32041 aaattcgctt aaaataaaaa tttttaatat aatttgttct atagtacatc tattttgatt
    32101 agtttacata attttattat tttgtacaat attaattatt tactaatatc ccatttaatg
    32161 aaaaagttct aggttcagta atttttacca taactaattt accaattaaa tcatcatttc
    32221 caccttcgaa atttacgagt ttattttgtc tagttctacc agcaaactta ttttcattat
    32281 ttttacttct tccttcaact agaacttcaa ctattctatc tttatatcca tcatttattt
    32341 ctgcagaaat cctatttaca gcttctaaca ccctattaaa tctatcatgc tttatatctt
    32401 ctggtatttg attttccatt ttagcagcag gagttccctg tcttttagaa taaataaatg
    32461 taaaagctga atcatatcta actttttcaa ccacatctaa agtgtccaat aaatcttctt
    32521 ctgtttctcc tgggaaacca atcattaagt ctgtagaaaa agcaatacct ggaacttcct
    32581 ttttagcttt ttctattatt tctaaataat attcctttgt ataatgtcta ttcatttttt
    32641 tcaaaaggct actgcttcca cattgtattg gtagatgtaa aaactcacac accttatcac
    32701 aatctctaat tgcgtatatt acttcatcag atatatcttt tggatgagat gtcataaatc
    32761 gaaccctttc aattccttct atatcattca cttttcttaa aagttctgag aatgaaactg
    32821 gattttcaag tgtcttacca taagaatcta cattttgtcc aagtaaagtg atttctttcg
    32881 ttccatttgc aacaagctct tttatctcgt taattatatc ttcaggagtt ctacttcttt
    32941 ctcttcctct tgtgtatggg actatacaat atgtgcagaa attattgcat ccatacatta
    33001 tatttacaaa tgcttttaat tcaaattttc tattacttct aagaccttct attacttctc
    33061 catccacatc ccatacatcc actaacattt tgtcagaatt tattgattct gtaagtaatt
    33121 gcggaaattt atatagatta tgtgttccaa atataaggtc tacatgttta tatttttttc
    33181 ttaattcctc tacaacatgt ggttgttgca tcatacatcc acatacagct attttcatat
    33241 ctggattttt tccttttaga tgttttaatt gtcctaagtt accatacact tttagctctg
    33301 cattttctct aactgcacag gtgttatata ttattaaatc actttcttca accatcattg
    33361 acatttggta tcccatctct tctaacattg aacataattt ttctgaatca tgttcattca
    33421 tttgacaccc gaaggtttga gtaaacacga gttttctctt tccagttctg ttgaagtatt
    33481 cttcattttg tctgtgtatt tgttctatat atttatcttg ttcttgtatt ttctctatag
    33541 gtattgttac ttgttttctt ttgctcacgt ctattcctcc tagttaattt cttatattac
    33601 acataacctt aattatatca aataagaaaa atcttttaaa gatattatta tttttctagt
    33661 tgtgttttaa tagtaaa
//
LOCUS       DAMJWS010000041        33212 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMJWS010000041 DAMJWS010000000
VERSION     DAMJWS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.041"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(129..1925)
                     /locus_tag="PC399_002036"
     CDS             complement(129..1925)
                     /locus_tag="PC399_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDF2592895.1"
                     /translation="MKPTMEILTKLQENSKKNHDEVFTRLYRYLLRPDIYYVAYQHLY
                     SNKGAGTKGVTDDTADGFSEIYIENIIEALKNEMYQPKPVRRTYIKKSNGKMRPLGLP
                     VFTDKLIQEAIRMILEAIYEPIFSDYSHGFRPARSCHTALAQIKKEFTGARWFIEGDI
                     KGCFDNINHAVLVEIINQKIKDARFLKLIRSFLKAGYMEDWKYHKTYSGCPQGGIISP
                     ILANIYLNELDRHVMKIKKEFDVATKARYTPEYTKLVGLRQRLHNKIKNSNGIEREKL
                     IEEYKSATAQMLKLPAKQCDDKKIKYVRYADDFLIAVNGNRQDCEKIKQELTEFISTT
                     LKMELSQEKTLITHSNTPARFLGYDVRVRRDQQIKPKGKFKTRSMNNKVELSIPFKDK
                     IEKFLFSNGIVKQRSDNGKLEPIHRPQLLNRTDLEIVTIYNAELRGICNYYGLASNFN
                     KLIYFNYLMEYSCLKTLAGKHRSKVSKIRAMYKDGTGKWAIPYETKTVIKKMYFANYA
                     DCKGKKFTDIVPQTAKNYSHDVTTLESRLKAKICEVCGCTESDRYEIHHVNKVKNLKG
                     KSEWEKIMIAKRRKTIVVCHKCHMAIHHGEKK"
     gene            complement(2579..3478)
                     /locus_tag="PC399_002037"
                     /pseudo
     CDS             complement(2579..3478)
                     /locus_tag="PC399_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
     gene            complement(3475..5679)
                     /locus_tag="PC399_002038"
     CDS             complement(3475..5679)
                     /locus_tag="PC399_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2592896.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            complement(5679..8129)
                     /locus_tag="PC399_002039"
     CDS             complement(5679..8129)
                     /locus_tag="PC399_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2592897.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYEMIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(8107..8505)
                     /locus_tag="PC399_002040"
     CDS             complement(8107..8505)
                     /locus_tag="PC399_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2592898.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            complement(8623..9126)
                     /locus_tag="PC399_002041"
     CDS             complement(8623..9126)
                     /locus_tag="PC399_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2592899.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            complement(9144..9647)
                     /locus_tag="PC399_002042"
     CDS             complement(9144..9647)
                     /locus_tag="PC399_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2592900.1"
                     /translation="MQETRVLVETGRTNGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSADMQEEYVELLTVFENLEALYICR
                     NVITVYHDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENKRYLVTEN
                     SIFELPE"
     gene            complement(9566..9862)
                     /locus_tag="PC399_002043"
     CDS             complement(9566..9862)
                     /locus_tag="PC399_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2592901.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     KDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGYWWKREEQMAKKQHHTGLTYR"
     gene            complement(9961..10734)
                     /locus_tag="PC399_002044"
     CDS             complement(9961..10734)
                     /locus_tag="PC399_002044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592902.1"
                     /translation="MKGIKVLMISDEMRVDILHAVFINKNFIKDEENCNYEKYLLELV
                     NKSIYFREKSNFAEYVPPKSENHGECDCNSPNYQMDFKLLESTTRLHASKELTGQIQK
                     FCDGVIGKCPPRRPNTQMTVTRLFASLRDYDCESLHSCLTEKYEYGTIEFDIQTYVKL
                     LTFKKNLFFFFPYKFSFNTCYNFKYALDSIRIALEKDFRESNLFREKYYAEYDTFLAY
                     IYEDNLIISKFEQDGKLQMIDCIYLFKSQTYSKLYEYTW"
     gene            complement(10794..11015)
                     /locus_tag="PC399_002045"
     CDS             complement(10794..11015)
                     /locus_tag="PC399_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592903.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(11016..11150)
                     /locus_tag="PC399_002046"
     CDS             complement(11016..11150)
                     /locus_tag="PC399_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2592904.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSISKEADYEMKQLEESEDN
                     "
     gene            complement(11163..12359)
                     /locus_tag="PC399_002047"
     CDS             complement(11163..12359)
                     /locus_tag="PC399_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2592905.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGIMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKYYQSGELIKHREDDR
                     EYMGSTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFIDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAILSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(12544..13938)
                     /locus_tag="PC399_002048"
     CDS             complement(12544..13938)
                     /locus_tag="PC399_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2592906.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSVFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNLRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGFLAQARQ
                     DGTATCEAKGDGSD"
     gene            complement(14032..14469)
                     /locus_tag="PC399_002049"
     CDS             complement(14032..14469)
                     /locus_tag="PC399_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592907.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFNVGEYKEYPHTEVRKLTSDEIKQYMSER"
     gene            complement(14571..14954)
                     /locus_tag="PC399_002050"
     CDS             complement(14571..14954)
                     /locus_tag="PC399_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2592908.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            complement(14970..15296)
                     /locus_tag="PC399_002051"
     CDS             complement(14970..15296)
                     /locus_tag="PC399_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2592909.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVEEK"
     gene            complement(15422..18466)
                     /locus_tag="PC399_002052"
     CDS             complement(15422..18466)
                     /locus_tag="PC399_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2592910.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPSTAVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDKNGNTDVVEVKAGTVYIRELSAPAGYKVDTT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAILPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETSDTKPQGSATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSAEWTSHK
                     HNTNAGKTSEDGVWFGTSEADDSKGALPYDTYIIEEMRCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDSEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFIADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKEIEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKAEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFNEKPEVPEKLETPQTPEKPN
                     RPSDSPKTGDSTNVMAFLVMLLTSAGGLAGTYLYKRRTMKKS"
     gene            complement(18709..20073)
                     /gene="rlmD"
                     /locus_tag="PC399_002053"
     CDS             complement(18709..20073)
                     /gene="rlmD"
                     /locus_tag="PC399_002053"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2592911.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHIETCVLLQ
                     RRTM"
     gene            complement(20248..22008)
                     /gene="pyk"
                     /locus_tag="PC399_002054"
     CDS             complement(20248..22008)
                     /gene="pyk"
                     /locus_tag="PC399_002054"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF2592912.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(22060..23019)
                     /gene="pfkA"
                     /locus_tag="PC399_002055"
     CDS             complement(22060..23019)
                     /gene="pfkA"
                     /locus_tag="PC399_002055"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2592913.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(23228..26797)
                     /locus_tag="PC399_002056"
     CDS             complement(23228..26797)
                     /locus_tag="PC399_002056"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF2592914.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(26954..27901)
                     /gene="whiA"
                     /locus_tag="PC399_002057"
     CDS             complement(26954..27901)
                     /gene="whiA"
                     /locus_tag="PC399_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF2592915.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(27918..28343)
                     /locus_tag="PC399_002058"
     CDS             complement(27918..28343)
                     /locus_tag="PC399_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2592916.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(28412..29518)
                     /locus_tag="PC399_002059"
     CDS             complement(28412..29518)
                     /locus_tag="PC399_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF2592917.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(29530..30387)
                     /gene="rapZ"
                     /locus_tag="PC399_002060"
     CDS             complement(29530..30387)
                     /gene="rapZ"
                     /locus_tag="PC399_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF2592918.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(30418..31131)
                     /locus_tag="PC399_002061"
     CDS             complement(30418..31131)
                     /locus_tag="PC399_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2592919.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(31135..32049)
                     /gene="murB"
                     /locus_tag="PC399_002062"
     CDS             complement(31135..32049)
                     /gene="murB"
                     /locus_tag="PC399_002062"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF2592920.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(32322..33092)
                     /locus_tag="PC399_002063"
     CDS             complement(32322..33092)
                     /locus_tag="PC399_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2592921.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         9114 a         7196 c         5004 g        11898 t
ORIGIN      
        1 tttcccacat agtagagtag gaaagcaccg cctttcctta tttctagggt aggtttgtgc
       61 agtcctctcc ccgaaccgtg cttgcatttc tcaatgcaca cggctctcca tctttgctcc
      121 gttacaggtt atttcttttc gccatgatgt atagccatgt gacacttgtg gcataccacg
      181 attgttttcc gtcttttggc tatcattatc ttttcccatt ctgattttcc tttaaggttt
      241 ttaaccttgt ttacatgatg aatttcgtat ctgtcacttt ccgtacaccc acatacttca
      301 catatcttcg cctttaatcg gctttctaat gttgttacgt catgggaata atttttagct
      361 gtttgtggaa caatatcagt aaacttcttt cctttgcagt cagcataatt cgcaaaatac
      421 attttcttta ttactgtttt agtttcataa ggaatagccc atttgcctgt accgtcttta
      481 tacatagctc taattttaga aactttggaa cggtgctttc ctgcaagtgt tttcaaacag
      541 ctatattcca taagataatt gaaatagatt agcttgttaa aattgcttgc caacccataa
      601 taattacaga tacctcttaa ttctgcatta taaatagtca ctatttccaa atctgtccta
      661 ttaagcagtt gtggtcggtg tataggttct aatttcccat tgtctgacct ctgctttaca
      721 attccatttg aaaataaaaa tttctctatc ttgtctttga atggaatact caattccacc
      781 ttgttattca tagaacgggt tttgaatttt ccttttggtt taatctgctg gtcacggcgt
      841 actctgacat catatccgag aaaacgagca ggagtattac tgtgtgtaat cagagttttt
      901 tcttgactaa gttccatttt taatgtggta cttataaatt ctgtaagttc ttgcttaatc
      961 ttctcacaat cctgtcgatt gccattcaca gctattagga aatcatcagc gtaacgcaca
     1021 tattttattt tcttgtcgtc acattgcttc gctggtagtt taagcatttg agccgttgca
     1081 gatttatatt cttcaatcag cttttctctt tctataccgt tgctattttt aattttgttg
     1141 tgtaatcttt ggcgtagtcc taccagtttt gtatattctg gtgtataacg tgcctttgtt
     1201 gccacatcaa actctttctt tatcttcatt acgtgacggt ctaattcatt aaggtagata
     1261 ttcgcaagaa taggcgaaat gatgccacct tgagggcaac cgctatatgt tttgtggtat
     1321 ttccaatctt ccatataacc tgcctttaag aatgaacgaa tgagtttcag aaaacgtgca
     1381 tctttaattt tctgattgat aatttccaca agtaccgcat gattgatgtt atcaaagcac
     1441 ccttttatgt cgccctctat aaaccagcgt gctcctgtaa attctttttt tatttgagca
     1501 agagcagtat gacaacttct tgctggtcgg aaaccgtgag agtagtctga aaaaataggc
     1561 tcatagattg cttctaatat catgcgtatt gcttcctgta tcagcttatc agtaaagaca
     1621 ggcagtccta agggacgcat tttgccattt gattttttga tgtatgtacg tcttactggt
     1681 tttggttgat acatttcatt ttttaaggct tcaatgatat tttcaatata tatttcgcta
     1741 aaaccgtccg ctgtgtcgtc tgttacaccc tttgttcctg ctcctttgtt ggagtagagg
     1801 tgttgatatg caacatagta tatatctgga cggagtaaat atcgatagag ccttgtaaat
     1861 acttcatcat ggttcttttt tgaattttcc tgtaatttgg ttaaaatctc cattgttggt
     1921 ttcatttgag gttttcctcc ctaatcaatt tctaatttta attcgctaag actgctttcc
     1981 ttcgccatgt gaacggtttt cccgtatccg attttacggc tttccctgtc ttttcagggt
     2041 tctcagacta ctatgaaagc tccgttacct tgccagattt tcagcgtcat ctcacttagc
     2101 atagccttgt gatttatcac tacaagagca ttacacatag ccattgtagg cattctggtt
     2161 taggtaatct ccagttgacg aaagtagtag gctggtgtga attgtcggat atgctttcgt
     2221 tccgttacta ctggttctcc agcgtaccgt atgagttaat tgataaccca ctgaaaggca
     2281 tttattttca gcgttactca taccacaggt ttcagacaag tttcctgtgt ccacacgtaa
     2341 cagaggtttg gaactcacat tcaacaaatc tagttttatc ctcatattca cttaaccttg
     2401 cagttcagtc gtgctctatt gtctttgaac aactttctgc tttcctgccg tgctattttc
     2461 ccgtacagtt ttcacctttc ggttaggcag gtggtttccc gtgttatctt acggggtagt
     2521 gccttttttt tctgtccccc tttctttaat ctacttctaa ctttcgtccc tctggacgca
     2581 cgataccgac gtgggttaca tacgaaccag cgttataggt ggaatggaaa aataccaaat
     2641 cgccagcctt tgcctgcgag agtggaagat gttgggtagc gtcatactgg gcttgtgccg
     2701 ttctcggtaa ggagatacca gcttttccat agcaccattg cgtcagtccc gaacagtcaa
     2761 aggaagtggt tggagaagaa ccaccaaaca catatttcca cccttgatat ttcaacgctt
     2821 cattcattac cttttgtgcc agctctcccg atacctgcgg aacagttaaa tactgattga
     2881 ctaattccac atagaacata tttccatagc cataccgcca gcctccgttc ttcgcaacgg
     2941 ctatcgggtt ggtataggtt actttctttc cacccgattt ctcacgggcg aagctctctg
     3001 caagattaaa agtgtgtttc tttccgtttc ctgccacata gcccacatag ccaccgccat
     3061 agttatagga ctgtatcgct acattcaaat cgtcgatatc ttgatttttg caggaagaaa
     3121 gcagggacgc aaaatactta cacccctgct tgattgagct ttccgtatct aaggaactag
     3181 gcggtaaacc aagactttct gaactctgca taacatcttc tgccgtaccg ccactttcta
     3241 cttgaatgat agccagtagt acattgacgt actcggagat accgttttct ctggcgtatt
     3301 tttctaccat agtctgatgt ttcaaaactt ctgcggataa gttcataccc gtaatgccag
     3361 aagaaaagtt gctgttttcg tcgtcgctgt ccgcactaat cagcacccca aagaaaagca
     3421 ccagagaaaa gagaatagga aacagacttc caatgatagc gatatgtttc agtttcattt
     3481 tttcttctgt cctttcttcg ttacaagatt acggattttc tctgtggtct gctggttctt
     3541 ctggacagtc tgggtctgct gaacctttgt cctacggtct ttggtgtaat tctggcgtgt
     3601 ttcctttgac acggtaactt tttctacatt ctgcctatat gtcgattggg tcgctttccc
     3661 gtcaggagta ctagaagata gcggacgttc tttgacaccg cttgtcttaa ctggttcgct
     3721 tgctttcgta gtcggagttg tggcagggcg tttgacttcc tgcatttttt ccgcacccgt
     3781 ctttgaaacg gttgaagctg tggctggtcg ctcatgggga cgggtagctc ccgttgtcgc
     3841 tgatccgtca gcctttcgct gtgcctgcct tgcttcttgt gccttttgaa gctccatacg
     3901 cttgacagcg atacttttct tatgctgttc ctgcttttcc aagcgtcccg tctgtctgga
     3961 ctgctgttcc tgcaccatac cacgcttgaa atcagacacg ctggactttg ccttttcctt
     4021 tgcggaatac accgcataag cggtctgtgt gggcatatcc ttgatattct ctttgacagc
     4081 gtttgccttg tctttcactt tatttttcgt atctaagaca gcacccacct ttgagcctgc
     4141 acgctgtccc atactggaag tggtattgcc ccgattttct ttagaagccg tattttttct
     4201 ttcggctctc ttgccagcaa cggcacttcc agctaccgca ccagccacac cgcccgaaat
     4261 accgccagca cttacagccc ttgcaatacg gtgttccata cgcctagccc gatgtcgcat
     4321 gaacagatac ggtctgcgga atatcctgcg tcccatgctt tgactgtcgc cagcgttgag
     4381 agaaaacata ctcattaagt cgcccagctt catatagata ccagcgaaac atactatctg
     4441 caagaacgcc accatgaaaa acggatagtc cgtggatatg ttataaaaca tactggaaat
     4501 gctaaacgca accgttacaa tgagcgttat tcctgcccgt gtcattatgg tattaaatac
     4561 cctcacgatt gcctgctttg ccatgctttc atagctcggt atcatggaaa gtagaaagct
     4621 gataggtaaa aacatagcga agataataaa aagtatctgg ctgaacaaca tcatacccgt
     4681 aagcaagaat acaaatatcg taataccaag attaaagaac aagaggaaga acaccatacc
     4741 taaacggttt accacctgcg gtatcgtcag attgttgttg tcgttgtcct cgatttctgt
     4801 tttcacgact tcttctctgg tctttccgtc ctcgtcctct ggacttgccg atacgagagc
     4861 ttcgacacgg tctgtcccga tttcctctgc gttgctgttt ccaaactgta agagtagcca
     4921 cggctgttgt acttgaatag aaaataggct gtcccgtatc aagtctacgc tgtccttgcc
     4981 ctcgctgtca gagttgggga gcatgatttt tgttcccaaa tcaagcgaag cggtactgat
     5041 gtctgatgaa aattcattta tcttcttgat gtagtcggga gcgtaggcaa taaacgaagc
     5101 ggacagcacg aacaccacca caaagttgat aacagcgtga agtgccttgc tggtttcccg
     5161 ttttatcagt cccgtatagg caacatacat tcccaccact aagataataa gaagcaggaa
     5221 accaacatag aagcccgtag aagaaaagcc gttctcggta acgcctgcaa gggtctgtat
     5281 gctttttccg atactgtctg ccatatcgtt aataaaatca agtttatagg cttcctgcac
     5341 cacatagcca gttgcattgc ttaaataaag gcttatcgtc cagacaaagt tggtaatgca
     5401 gtaaagcccg tactgtaccg attttccgat accgtccagc cagttccacg gaagccacga
     5461 ccagctatta tcgacgaaaa aatcaagctg gtagttggaa agcggatatt ttgaatatag
     5521 attttctgcg tttatggtat cgtccacaag ccccgtcgca tgagccaccg tccccagaag
     5581 tgaaagcagg ataagggaaa gtgccaccac gaacagagcc attttgagaa agtgaagtat
     5641 cttccttttt gtgaacgcac tctgtatcct ttctttcatc actccacctc atttctctgt
     5701 acgggcggtc tagtatcaaa ggcgtgtagt aattcttcaa agactgggtg tatctgcacc
     5761 acaccgacac gcccgtataa gtcctgcaat aaacattgtc cgttctccaa atcacgcaag
     5821 cgtttctggt tgttttcgtc gtccttgtcg ataccgaaaa attctaaggt ctgctttatc
     5881 tcgttgatgt cagtagaacg aaatgcaaac ttcaaaccga tattgttttt cagactttcc
     5941 tttgacacgt cataggcaga ctgggtaacg aaataaacgc ctgcctgcat agctcgtcca
     6001 gcacgaacca gcttatttga tagggtttcg ccttgtgcca catttaagaa cgcccacgct
     6061 tcgtccaaat ctacaatctt aaaaatgctt ctgtccgaat ggataaaatc aagggcaaag
     6121 gtactaatca caatcagcat agacaccgac aataattcaa tggtcgtgta ttcctcaaaa
     6181 gtggtatctt tatctggcag tacaaggtct gctacttgaa tgatattgag ctggttatca
     6241 aggcttatag cattttctac cgtcccgtct gaaaacagca ggtgtgcaaa gtcgtagtcc
     6301 gtgaagctgt cgatatggtc tgcgatattg cgtgagatag cggtatcttc ttttctcaat
     6361 tcctcaataa cgtgtaacag tcctctattt tcactctggg taacagaacg caccgctttt
     6421 cgtaacacgg ggaatttttc gccgtcccta gaggaaatac ccgtaaggaa tgttaagatg
     6481 tcgattgcca gactttcagc gtctttcaca ttcttcataa tcacaaatgg gtcaagaagt
     6541 cctgcattgt ccttgtcgct ggtaaggttt acgatattga tttcatgggc tatctctggg
     6601 agcgtttctt tccagttgcc acgctcactt ttagggtcta acagaagtgc ttgaccgcca
     6661 aacagcaccg cataatatac aagtagattg ttgcagaatg actttccacc gccaagactt
     6721 cccacaaaag cagaagcaag ggcattagta actgtgcctt ttactccctg cgaagctaaa
     6781 gacggttgca ggtacacatt tcttcccgta tcaacggaat agcccatata gatacccgtg
     6841 ttttccccta actgctgggt cgcaccaaaa ccaagcccag ccaagaagtc tgactttaca
     6901 tactgcacat agtcattgat atatcgcttg ctggcaggta aaaattcaga atgaagtccc
     6961 aacatatcgc cagcaggacg caccagtttt acattgaggt cgtcgtaaaa gtccttgact
     7021 tcatcacaac ggcgtttcag ctcgtcaaga tcgggtgcag acacccgtat gacgtagctt
     7081 aacttataca tactttcttt gctctggtct aaatcggttt ccagctcgtc cacgctgtct
     7141 aaagcgtcca ccacatttga gctggtttca cttcctgctt gataggcgtg attatccaaa
     7201 tctttcaatt cctttttctt gttgcggact gtcgttaatg ccttacggtt ctccacgatt
     7261 tctacattca tagaagtatc aacggggaat gtgaattgct gttgctggaa atagaagatt
     7321 tcagaggacg gaaaatccaa ctccccgaca attacattga cggtaaaata ggacacatag
     7381 ctttccttgt cctcatgttc caatcgtaaa taccgctggc tttcctcaat cacacacctt
     7441 gtcggacgga taaggtcgta gtattttatc agcgtttctt tcttcaattt cttctttggt
     7501 agctggtact cataatcttc ataggcgata ccgtccctgc cgtaaagatg ttccatgaga
     7561 tacccaaaat catggatttc caagcgacgc accttaaagc gacgggagat tttattttcc
     7621 agtaactttt ccattttcat gtaacggttg atttcatcat tcggcatgga aacaaaatca
     7681 ttcatcagcg tgtggttcac ttcatgaaga aattccttga acgtcagcca tgccgatttt
     7741 ttgatgttct tcagattgag ctgttcttcc gtaaccatga gcttaaagcc aagaaaaaag
     7801 cggtagtcca cttgattgtc cccaatcatt gatactaacg cttcggtctg ttcgtctatt
     7861 ttttggactg ccacctcacg aagtttgcct gttaccagtt tctttgactg ctcctgcata
     7921 cttctaatgg agctttctgt ggcaatctgc aacgcatgaa tttttccctc acgggactgt
     7981 gcgattagct gtcgaaaact gtcatgcacg ataaatttct gctctgcgga tagaaaagaa
     8041 tagttgtacg gtatcatctc atagtaggcg aacacctcat tgtccttgtt ccagacaagg
     8101 ttattgtcaa tatattttat cgggaacata gttcacactc cttactgccg tgattgtttc
     8161 attcaatatc tgcttatgca gttttacggc ttttcctgca taagtgattt taggtcgcaa
     8221 ggcataggtt atctgtgatt tcaaaaagct gtacggcttc tttccgtcaa aggttttctg
     8281 cgacataaac caagtgagag caacgggaat accgaagtat ttgagaaatg ctccctcaat
     8341 catggaaagt gggggcatat ccccaaacag aatgatgata aattccgtaa tcacaaacca
     8401 tgtaatctgt gtaaaggtaa cgggaaaggg taagttaaag tcattgattg catataagac
     8461 tttttccacg ttccagatac ccgtgtagct tttaatcttt ttcaagttct ttcagcccct
     8521 ttcgtaagtt cttcaaagag aaaaggacag ccattttcag actgtccctt aaagagaaat
     8581 acgctgtcag tagcaacaat gcttgttgct gatcttccag cgttaatatg gtatctcaca
     8641 gatacctcgg cttgtttcaa tgaatcgacc acccatatcc aaatctcgcc cgtaggcttc
     8701 atagtcaata tagttctgta agttagctgg aatgtcccca agtgcgttca attcgtctat
     8761 gtagtagtag gcaacatctg tcatggtttc acaatcggga taaaaataaa tatcgtcctt
     8821 atgttccacg acttcttcca gcgtcccata gtggctgata aattcgtcca gacactctac
     8881 gatatagtcg ggaagttcct ctatcatttc atacatttca ttgagttctt caatggaaac
     8941 atattcccct atgtcgatag gaaagttgtc ggtatcatgg acagcgtatt cttcgtactg
     9001 ttcattcaag ccgatttttt cttttacatc ttcctcgtca atagggaaag tgaaccacgc
     9061 accgactaaa tatccctcat tgtatctgcc aagattagca atataaaccg ccatatcatc
     9121 aatcataagc accacctcat ttcctattct ggcagttcaa agatactgtt ttccgttaca
     9181 agatagcgtt tgttttcaag taagtgagaa gcgtaggctt caaaatcgaa atagtatgct
     9241 tgacagtcct cggataaatg tttgaatgtc gggtcgttca tcagcttttg ccttgcaaca
     9301 tctatcatgc ttttacagtc gtgataaacg gtaatcacgt tcctgcaaat ataaagtgct
     9361 tctaagttct caaacacggt aagaagttct acatattctt cctgcatatc ggctgatagc
     9421 tggcggtaca taaaatctaa ttcgttaagc tggtacacgc tggtgtgttc gtggacttca
     9481 tcagcataag gcaacacctt ttcgataatg cggtaatctt gactttctgc accgacacct
     9541 aacttttctt caaactctgc aacatctatc ggtaagtcaa accagtatga tgttgtttct
     9601 tcgccatttg ttcttcccgt ttccaccagt acccttgttt cctgcattgt tcctcacgtc
     9661 ctttctgttg tttcatcaca tcttttaagg tctggtatga cataccaaac acatatttag
     9721 aaaaatcttt caactgtttt tcttcatagt cggcagggtt cagccgtttc atttcatgcc
     9781 acaccttacg cttgtaagag ggcaggtcgg aaatgtattc acagccgact tttgcctgca
     9841 tatctttaaa aatatcgttc atttcatcac tccaatctga aatttaggta agaaaaaagc
     9901 agtcaatcct aagactaact gctttgtgtg taagtatgaa tttgtttaag tgtaactgat
     9961 ttaccatgtg tactcgtaaa gtttactgta tgtttgactt ttaaataaat atatacaatc
    10021 aatcatttgt aacttgccgt cttgttcaaa tttacttatt atcagattat cttcatagat
    10081 atatgctaag aaagtatcat actctgcata gtatttttct ctaaaaagat ttgactctct
    10141 aaaatctttt tctaaagcaa ttcttatact gtctaaagca tatttaaagt tataacaagt
    10201 attaaaacta aacttgtatg gaaaaaagaa aaataagttt ttcttgaaag taagcaattt
    10261 cacataggtt tgtatatcaa attcaatcgt tccatactca tatttctctg taagacaaga
    10321 atgtaaactt tcacaatcat aatcccgcaa cgaagcaaat aacctagtta cagtcatttg
    10381 tgtatttggt cttctcggtg gacacttacc aattacacca tcacagaatt tctgtatttg
    10441 accagtaagt tcttttgaag catgaagtct tgttgtgctt tcaagtaatt taaagtccat
    10501 ttgataatta ggtgagttac aatcacattc tccatgattt tcagatttag gtggtacata
    10561 ttctgcaaaa tttgattttt ctctaaaata tattgattta ttaactaatt ccaacaagta
    10621 tttttcataa ttacaatttt cttcatcttt aataaaattt ttgtttatga atactgcatg
    10681 taaaatatcc actctcattt catctgaaat cataagcact tttattcctt tcaaaaaata
    10741 atttatcgta cccacattat aacattctca tattacagca acaataaaat tcgttatgct
    10801 ccaataatct tattgaacag ctctaacagt acatctttca caccgccagc gttgaatact
    10861 aagccgacag caataagggc aactaccaag aagccaataa gttttgaaaa ctcacgcttg
    10921 aaccctaagt agataccgat aaccacaatc gccataagca ctaagctctg tgcatttgat
    10981 aaaaaccatt gatacaaatt ttgtccgaaa ttcatttaat tgtcctcgct ttcttctaat
    11041 tgtttcattt catagtcagc ttctttgctg atactcaaaa tacacatcaa gactacgccg
    11101 atacccattc ccatagatac cagcaggaag tcttttacta attcccacat tttttatcac
    11161 tcctaacttt ccaccaaatc ttttgcaggg gtcgtctgct gtttgattat catttcatgc
    11221 ttttcactca agattgcctg cttttcaatc gtttccatgt agtctgtacc gtttccctta
    11281 tcaatctttt tcagcatttt caaggtcggt gctacctgtc tttgtaccca acgcaatgtg
    11341 cggtctaagg tgtaaggttc tggttttgtc gtcagtttca gactttgccg attatcgcca
    11401 ataaaccaag cccagcggtc attgagtttc cagtcatttt tccgcttgtc tggttcttca
    11461 tcaataaacc gcacatactg gttgatgata gaaaaagcgg tctgctctgc gtcatagtag
    11521 gtcagcaaat ctcgtactgc atagtaagca cgttcatttc taagccgtat ctcaaaacgg
    11581 ttgataatgt cggcttcttc cagaggtgta cctaatttga catactgctc atagtccttt
    11641 tcatagatac agaaatacac atctgatttc agcgaaccaa gataaagggt actccccata
    11701 tattctctgt cgtcctctct gtgcttgata agctcgcccg actggtaata cttatagctt
    11761 ctggactttc cgatatattc ccgtttcctg catttctccg caagctctgg aatatccaaa
    11821 atgcccgtat ggtcgttgat agcaaggtcg atacgcttca taatgcctcc gtctatgagt
    11881 gcgtccataa gaaagtcata ccagcttctt tgctgtgcca gcaggtaact ttcaaactgc
    11941 ctgcaacccc gaccctttaa ctctaaaagg acaccttttt cttcgtcagc cgacgtatag
    12001 ataaagatgt cccctaatga ataatgctcc gtataactgt aatgtccgta atcttcatgg
    12061 agcatataat tgatattcag ttttaatatg tctttgatga tgtgctgtat atccagcgtg
    12121 ggaaaacgaa ttttcacata atcaaacagc atggtaagcg gtgcttctgg attgaacctt
    12181 gccagttctt tatgcaaagt ttccagaagt tcttttgacg gcttcatttt tccgctttct
    12241 atcttgttta gatattccct tgtgatacca gaagctaccg ccagcctgcc ttgtgagata
    12301 ccgtaagcaa tccgcttctc ccgtaattct tttatccatt gttcttcatt cagaaccata
    12361 cccccaatct gtcaagtgtg aagtttttta aggcgacttc acagccgatt tttgctagga
    12421 aaccatgcca gaagccttat gtttcctatg tttttttgtc tattgtctat ttgcaaacgt
    12481 acccctctgt tagataccga ggggttttac ctgctggcgt ggcttacgcc acaccagcaa
    12541 cggctagtcc gagccgtcgc ctttcgcttc gcacgtcgcc gtcccgtcct gccttgcttg
    12601 tgcaagaaaa ccgattgtct gcaaaaaatc atgtcctttc gggactaaag gcgtgtaaaa
    12661 ttcacttatg acgcttgttc ccacatcaca atagccacgc cctttgatac gcttttggaa
    12721 aaactgcttt ttcacatctg aaccaaacag cataccgtaa cctaactcgc tgatacgccc
    12781 caatcccaca cgtaaattga agttatctct gataccgtcc gagaaatatt ttgcgtctgg
    12841 acgctggcag gcaacgataa ggaaataacc tgcttgtcgt cctaacatga cgattttctt
    12901 taactggcta agtaagccca cactttcttt tgtccccagc atttcaaaaa acgccacata
    12961 ttcatcaaag ataagaaagc agggtggcag tcccagatag gcgtaatttt cgcccgtctt
    13021 atagttcggg tgtcgtttca tttgctcgct tcgctgtacc atgccctcat aaaaagcatt
    13081 gacacagtct atcatttctt ctttggtgtg atacacgttt cccatgactg ttcccaagtc
    13141 tgctaagtcc gcatttttcg ggtctaagat gtaaaggaca gcgttggtat gcagtagggc
    13201 ttcaatgagc gtcagaagaa aatatgtttt accgccacca gtcccaccag caatcagagc
    13261 gtgagggagt gcgtcatatt cccagacaag atttttcatc agtctaagac agccgttctc
    13321 tgcccgtact tcatcaatgg taatgcggtt cgctatcata tcataaagca gggtatattc
    13381 gatatagccg tcatgcaggg tcttgtcggt cagctcacaa tacaagccac tttccaattt
    13441 atcctctaac cgtaaaagct ggtcttgata tttccccagc gtaatttcac agcggatatg
    13501 aagcagtccc ttttccattt gataataaat ctttggaaac cagacgattt tttcccttga
    13561 tctgctttgc aggtcagtaa aaaaaacgct gtcttgtacg gtatcagctt cataccactt
    13621 gttttccagt atcattcgtg ccagcttttg acggtgcagt agctttttga aactgtcgta
    13681 acagaagcgg taatacagaa aagcgaccaa tgcacaaaca ccagtcgcta tcagtatggt
    13741 tatgaagttg taaagggaaa gcgtaaagcc attgtctaat aagctgaaat gttcccagtc
    13801 ggtacgcatg agctgttttg tatttaatag caggataacc gctacgaaca caaacaggag
    13861 tgtccccatt gaaaagcgat agacaaggtt cttatcgctg gctctgatac ggtgtccttt
    13921 gtttaaaatc attctcataa atcaatcact cctttctggg tatgaaaaaa gcagtcaatc
    13981 gtatagacta actgctttgt gtgcatggat atgaagttgt caaactggaa tttaacgttc
    14041 actcatatat tgttttattt catcagaagt caattttcgt acttctgtat gtggatattc
    14101 tttatactct ccaacattaa acacaggtgt catttgtttg catttcttgt ctttattctt
    14161 tttcaagaat agatgtaaat cctcattccc ggcgaatatt ttataagata ctctgccttt
    14221 actgttctct atctcataaa taccacatga acttttcgtt gtataatcgg ctgttcgtaa
    14281 ataaagtttt gcaatttcca atgacttaaa aggaaaattc catctttgta atcctctgtt
    14341 tgggtcatgt tcaatacaaa tgcaacgtcc acaagttcca caaatatatt gagtgtggtt
    14401 ttctaagcag tccaagggat ttagtaatgg tgttattcta ttttcatcag agtaacattc
    14461 ttcacacatt tttcttttcg ctccttatca aattctaatt tgtcgctttc ttcataccca
    14521 tattataaca gttccgtata tcctctacaa cgaaaatcta ttcagcaagt ctattttttc
    14581 ggttgtccct gcggtgtatg gttctgtgaa gtgcctgtgt tctgattgcc tttattcttc
    14641 agtacaatat cttctgcctt tacataccag tccacatctg cacccgtata ggtttttcgt
    14701 gataccgtat cggctacggg attgacaagc tccacgactg cattgtaagg aaattccctt
    14761 aacggtactt ctggcggtac ggacacgggg ataattccac catgcagact gcacttcaaa
    14821 tcatagatac gctttttaag ctgggtactc ggtgtcccgt cctcattttg caagaacaca
    14881 tcacgcaccg ctgtaaattt taattctcca aatgttttct ctttgtcaat gacaaatcca
    14941 tttgataatc tcataaattc ttcactcctt tacttttctt caactgctac aatatcatca
    15001 gctactaata cataatcggt atgtcccaca tccccgattg cgtaccctct gccgtataac
    15061 ttcggattga caagttttac tttctgttca tacttgaaat gcttttcgcc agcctgcacg
    15121 ggaatttcca ccacaacatt ttcgcctttc tgtacgtcag aataaaggtt atagcttcgt
    15181 ctggctatga ccttgcggtt gtttttatcc ctttcaaaga taggctcgct ttcgcctgca
    15241 aattcaagag ttccaaaaga ctgtgccata tctggcacga catatttcat ttccatattg
    15301 ttttacctcg tttctttcta tatttgataa gtttatgatt gtaatttttt attctggctt
    15361 gtctgccgtt tgacagtttc ggcggagaac gtgatttttc tttcatcata ccgttaccgc
    15421 cttatgattt tttcattgta cggcgtttgt aaagatatgt tccagccagt ccaccagcag
    15481 atgtaagcaa catcacaaga aatgccatga catttgtact gtcgcccgtt ttaggactgt
    15541 cgctaggtct gtttggcttt tctggtgtct gcggtgtttc gagtttctct gggacttctg
    15601 gtttttcatt aaaagtaatc gtctgtccct cgtcctcaat atccttatgc tcggtaactt
    15661 tcttcggctc gtctggattg cttaaatcat acaattcttc aaaagttaca agctgtttgc
    15721 cgtcaaggga agtagcgtca aaggtaaagg caacttccgc tttcatagtt tcgctgtcag
    15781 cggtaaaggt gtaatcactt tccacacgtt tcccattgat aagaagttct gcattttctt
    15841 ctttcaacat ctgccagcct acaagtttgt actgtgtacc gacttccaag ccctctaagg
    15901 ttacggtatc aatgattgta acgtcttttc cagcttcaat ctctttgttg ccgtctttgt
    15961 cggtagcagt cgtatgtatc ttgatgatac gttctgtaat caatacagtt tgtccgtcgt
    16021 cctcaatgtc tttgtgttcc gcaactttca cgggttcgtc tggattgctt aaatcgtata
    16081 attcctcgaa tgttaccaga tttttaccgc caagctcgga cgcattgaat gtataagaaa
    16141 tttccacttt catttcttca tcatcagcga taaaagtata gtcattttcc acacgttttc
    16201 cgtcaatgat aagctctgca ttttcttcct ttaacatctg ccagcctttg agctgatatt
    16261 ttgtaccttt tgtaagcccg tctaatttga cagtatcgac gattgttacc tcttttcctg
    16321 caaggatagt cttttcgctg tctttgctgg tcgctgtggt atggatagag atttcttttt
    16381 cgtattcatc agtcaaagtc cctaaatcaa tcacaaggtt atttcttgat accacgattt
    16441 caaaaggtgg gataagttca aagcctttgt tactatcgca acgcatttct tcaatgatgt
    16501 aagtatcata aggcaatgca cctttgctgt cgtctgcttc agaagttcca aaccacacac
    16561 cgtcctcgct ggtcttgcct gcgttagtat tgtgcttatg agaagtccat tcagcagaag
    16621 tggagaattg cccgttatca tcagtaacta caacatgact ttctcccgtc gtcttgcttg
    16681 tgatcctaaa gggaacatca gcaagacgct tgtgtgtgcc tgcaccgatt ttcactccct
    16741 caatatcccc acgcttaatc tgattgtaga tagagtgggc ttcgtcggtt aagtccacga
    16801 tttttccatt ctctgtgatt gcaaagtcaa tcggcttcgc accgtctgtc aagtaaccgt
    16861 cgggagcttc actctcaacg atacggaatt tcccataagg taaaaggtca gcagaagtag
    16921 aagccacacc ctcaatatct gtacgaattg tctttaccac ttcatttttc ttatatagct
    16981 tgccctcaac caaaacagaa ttgtcattta aggaaatgac gtcaaaagca gtatctttca
    17041 aagtggcact tccttgtggt ttggtatcgc tcgtttctaa atctcttttc tgaattttca
    17101 caccgccacg gataaccttg tctgatacgg aaaactggtt acttcccgtc aatacagcaa
    17161 ggtcgccgtc ctctgtaatt tgtgtaaggt ataagccttt tatctgttca gacttatcgc
    17221 cagcctgcat atatgcaccg tccagcaagt agccgtttgg agcttttgtt tcctcaacag
    17281 atagtgttcc taaaggaaga atcgctttgc cgtcctgcat atagaagctg tcgccagata
    17341 ccttgtaagc gtccgctaat tttgtgatgt agtgagttgt cccgtcactg tctgtttcag
    17401 cgattgtctt tgtaacccat gtacgagtag cttcggcagg gagattgtct ttgttataga
    17461 agccagcata atacttccat gtaaactctg caccctctaa agaagcgttt ccctgcggat
    17521 tgtctttctg cgtttccata tcaatcttga aaagctcaat caaggtgtcc gttacttttg
    17581 gtgtatctga tacgttcaag gtcgctgtct ttccagcttc aacttttaag gaatacacgg
    17641 ttgtatcaac tttatatcct gctggtgcgg ataattccct aatatagact gtacctgctt
    17701 ttacctctac aacatctgta tttccgtttt tatcagtcgt aagggtggca agctgtttcg
    17761 tgcagtcctt atcagaaaag acaccatagg tcgcaccagc gattgagtaa ttcccgttac
    17821 cgtctgtaat gctggtatta ctggaagtct tttgcagttt tgcatttccg acatttaact
    17881 tcgcccagaa ttgtcccaat tcctgcccct cgccagagta gatataaccg ccacattcat
    17941 agcgtccttt attttctttc acaaaggctt tagcaccagc gaaaacttcg tcctgagttg
    18001 cttttggtat ttcatcataa gaagctcgca cgttgtcgca ttgccagcca agatgtacgc
    18061 tcaatctctg ccagacaaca cattgttcca acagatagac ttgcttgtag ttcaattcct
    18121 tgtgtgcttc gccatactgt ttgacatact ctaaggataa cgccacatct gaaatctggt
    18181 cggcactcat acgggtgctt gcgtcagctc tggtcttgta gccattctta aaatctgtat
    18241 tgatgtctat acagtaagcg tcctcgccct caacagtcaa atgtccctcg ttaaaggtag
    18301 aaccaatcga accgtcattc attacttttt ctacgatacc gacacgttcc tctgattccg
    18361 tccagtattg cttgctttct gcatggacgg cggtactcgg taaagcagta acgacagtcg
    18421 caaaggcaag tactcccgta cacaatcgtt tcaatatctt tttcataatt ctaaatgctc
    18481 ctttcatttt gggtaaaaaa atagacgctc aatattgaac gtcttacatt gaaaagggat
    18541 actttttaca agtacccctc aaatctaata tttaattgtt tgtgttccca cactctatat
    18601 caacaactac tgaaacattg atatagtttt ttatctcttt atttctgatt ttatcgccct
    18661 cgctcgtaaa gactatatgc ttaaaaaagt gcctaaaatt aaggatttct acatagttcg
    18721 tctttgtagc aacacacaag tctctatatg tgtagaatga gggaacatat ctacaggttg
    18781 tgcctcaaca catttaaatc cgtgaacatt tagataatct aaatcccttg ctaaagtaga
    18841 aggattacaa gaaacataga caattctttc cggattcatt gaaacaatag tctctaatac
    18901 cttttcgtca catccctttc taggtgggtc tacaacgact acattggcag tcttaccctc
    18961 tctatacatc ttaggtacaa tttcctctgc ctttccaaca taaaattcag cattatgtat
    19021 attgtttaat ttagcattta tcttagcatc ttttatggca tcatctataa tttctattcc
    19081 ataaaccttt ttagcctttt gtgccaaaaa taaagatatt gtacctatcc cacagtaaat
    19141 atcaaaaaca gtatcagtat catttaaatt tgcatattca agagccttat catacaatac
    19201 tttagtttgg acagggttta cttggaaaaa tgataatggt gaaatctcaa acactaagtc
    19261 tcctatataa tcgtttattt ttccatctcc atatataact atattttctc ttcctagaat
    19321 agcattcgta tctttttgat ttacatttac aaccaaagtc ttaaatccag gtatattttc
    19381 cttaagcact gaagctaatt ctttaagata tggaagtttg tttccatttg ctactaatac
    19441 aaccattact tctttagttg taaaacctat ttttgttact aaatgcctaa tcaaccccgt
    19501 gtgagtcttt tcatcatata tgcttacctt ataggccctt atatatgttt ttattatttt
    19561 aacaatttta tcatttatct catgttgtat tacgcatttt tctgtcgata ttacatcatg
    19621 agtcttcttt ttatagaagc caataattgg aataccatta ttcttttgta ttggaaattg
    19681 tgctttattt ctatatctat atggattatc cattcctata gtgtcatgaa cttctacatc
    19741 ctctaactta cctattctcg aaagtacttg tttaacttca tttgttttca tttcaagttg
    19801 tttattataa tctaagtctt gtatctgaca ccctccacaa tccctaagat tgtcactaca
    19861 cactcttttc accctgaatg gagacttttc tattatctca acaatatctc ccacagcata
    19921 atttttcttt actttattaa tcctaacttt aactttatca tgtattaatc cgccctctac
    19981 aaaaactgta ataccattgt actttcctac tcctactccg ccttgaccaa tgtctactat
    20041 atctacaata tacatctcgt ttttcgaaag cataaatcac cctccaatca atctattata
    20101 tttaaatctt tacaaacttt tttctttaaa tttatattaa aatattgcta ttaattaaac
    20161 tatatttttt actaagctac aatattttat tttaccaaaa aaaaaggagc ttataaagcc
    20221 ccttagtaaa taaatttatt atttttctta taaaactcta gttgaacctc tatataccac
    20281 gcctcttgat gcatccacag ttacaacttc tccatctttt actagatttg ttatatcagc
    20341 tgcagacaca ataactggta tatctaaatt tatacctaca atagctgtat gtgatgtcat
    20401 tccaccattc tcagttacta cagctcctgc tttttctata taattgttca tttcagcatc
    20461 agtagcagta gaaacaagta tgtcgccttc attaaagtct acacaagcat ttccattctt
    20521 tattatacga actttacctt ctactgtttg agtacctaca cctatacctt gcacgatttc
    20581 ttcgcttatt acatgaacct ttattaaatt agtagttcca cttactccta ctggtactcc
    20641 agcagttata actactaatt ccccattatc tatataatca gcttgtctag ctgcctcaat
    20701 tgccttttct ataacttcat ctgtatttcc tgcaacagaa gacttaactg gatatactcc
    20761 ccatgtaaga gctaatttat tcatagtctt ttcattattt gtagtagcta ttattggaga
    20821 ttttggtctg aatttagata ccattctagc agtatatcct gaagaagtag atgttattat
    20881 agctgatgca tttaaatcta ctgctgttgt acaagttgca tggcttattg catctgttac
    20941 agtaacattg ttagtaccat tttcctttag taatctatca taatctaaag tctcttctgt
    21001 tctttttgct attgtagcca tcatttttac tgcctctaca ggatattttc cagcagcagt
    21061 ttctccagat aacattatcg catctgttcc atcatatata gcattggcaa cgtctgtaac
    21121 ttctgctctt gttggtcttg gatttcttat catagaatca agcatttgag tggcagtaac
    21181 aactggcttt gctaattcat tacatttttt tatcatcatt ttttgaacta ttggcatctc
    21241 ttcagttggt atctcaacac ctaagtcacc tctagccacc attataccat cagaaacttt
    21301 taatatttca tctaggttct ctacaccttc ttggttttct attttagata ttatttgtat
    21361 atcagttgca ttattgttct ctaatacttc tcttattgct agtacatcag atgcctttct
    21421 tacaaaagaa gcagctatat aatctattcc ttgacttatt ccaaactcta tatcacttat
    21481 atctttagga gttattgctg gtaaatttat cttaacacca ggtacattta ctcctttatg
    21541 atttttaact attcctgaat tttcaacaac acaaactata tcttctccat ttatttcttt
    21601 tactcttaag ccaactaaac catcatctat tagtatagaa tctcctgatt taacgtcttc
    21661 agcaagacct ttatagctta ctgtacacat ttctttagtc cccataatat ctttcatagt
    21721 tatagtgaac ttttgccctt cttctaaaaa tacctctggg tcttcaaagt tacctgttct
    21781 aatttctgga cctttagtat ctaataatat agcaactggc ttatttaatt tttctctaac
    21841 tttctttgcc atatcaattc tttctttatg ttcttcatgc gaaccgtgag aaaagttaaa
    21901 tctacacaca tttagaccat tttgcataag ttgagttaaa acttcctcac tttgtgatgc
    21961 tggacctagt gtacaaacaa tttttgtctt ctttacatta tttaacatat ttgctcctcc
    22021 tgagatatta ttccacagat ttatttttaa tactaaaact tatatagata atatttttgc
    22081 catctcataa gcttctttat caaatacttt cttttgagct aaagcttcac ttatttctaa
    22141 atcaattatt ttgttttctt ttattcctac aactctagct gattttccat ctaataataa
    22201 ttctactgct cttactccaa gtctacttgc tagtattcta tcagatacag ttgggcttcc
    22261 tcctctttgg acatgcccca atacagtgac tcttaaatct gctcctgact cttttttaag
    22321 ttccttttct aaatcactag cacttccgac tccttctgct aaaacgatta tactatgtct
    22381 cttacctctt ttttgagtag tctttaatct tagagcaact tcatctactg taatttctac
    22441 ttctggtact attatagttt ccgctcctcc tgcaagacct gcatataaag ctaaatctcc
    22501 acagtgtctt cccataactt ctactatgtt aactctttca tgagatgatg atgtatctct
    22561 tatcttacct atagcatcta taatagtatt cattgcagta tcaaatccta tagtataatc
    22621 tgtatatgct aaatcgttat ctatagttcc tggtatgcct atagctggga atcctaagtc
    22681 actaagtttt tgggctcctg caaatgaacc atctccacca attacaacta agcaatcaat
    22741 tttatacttt ttaagaattt ttactgcttt taatctacct tcttctgttt taaattcttc
    22801 acatctggaa gattttaata tagttcctcc cctgtgtata atatctccaa cagaagataa
    22861 gttcatttca gttaaatctt cttctagtaa tcccttatat cctctattga ttccatatac
    22921 tttgcatcca taatatattg ctgatcttac aaccgctcta atcgcagcat tcattcctgg
    22981 agcatctcct ccacttgtta gtaatcctat agttttcatt ttttacctcc ttggatttta
    23041 tgtcaatttt ttattataat cattgttatt gtattctaat ggactttttt tgtccctata
    23101 tcacttttta atcccatcaa gactaaaaaa attaaatgtc cttaaatgtc cattttgtga
    23161 tattatatca caaaacggac aatatttttc atattattat aatatattta gatttttata
    23221 taaaaaatca agcttttact tttatgttat ctttaggaag taactcttct aagttattta
    23281 taaattcatc acatatacaa gtcaatgccc cggtcatttt atatgcttta cgtttaccat
    23341 ttatattttc atccataggg tataagtata tactgtcatt acctggatat tgtttggcta
    23401 tttctacaat attattagtt gtatcagtat cagacatact atctattttc aaatacaatt
    23461 ttttaccact ttctggctta atattttcta catttctagt tcttcctgaa ttgtagtttc
    23521 tatctgcaaa gtcagtatca tcatttatat cttttatctc tctagctata agttttgcat
    23581 tttcgccttc ttttatactt aatgttcctt ttacatatat tatattatct tcattcaaaa
    23641 tgacattata cttttgtaat aattgaggaa aaactattat ttctatagca ccatacaaat
    23701 cttctaattc aataaatgcc ataatttcat ttcgttttgt tgttttaata gttttagtta
    23761 caatcattcc acccattata acatctgttt cattcatttg taggtatgac tcttcttcct
    23821 cttttaatga atttaatttt ccattatcca tagatgtttt ttcttgtagt tctttttcaa
    23881 attgtgataa aggatgtcca cttacataca ttcccaagac ttctttttca aggttaagtc
    23941 tctctctttc ttcgtactct tgccttttag gcaaactgta tatttgttga aaatcactat
    24001 cttcctcact atctccaaaa gcatcaaata aagaaatttg accttgaaca tttttctttc
    24061 tatccataga tacactttct aataaacttt cataaccagc catcagacta gctctatttt
    24121 cacttatatt atcaaaagct ccacatttta ttagactttc tattactctc ttatttgtat
    24181 ctttttggtc taatcttttg actaaatcta ctaaatctac aaataatcca ttagattctc
    24241 tttcagcgat tatattgttt attatattta cgccaacatt ttttacggct gctagaccaa
    24301 atcttatatt gtcgccttca actgaaaact ttgaaaaact tttgtttata tctggaggta
    24361 atacatctat ttctagtgct ttacattctc ttatatactc aactacctta tcagtatttc
    24421 ccattacact ggttatcaat gctgccataa attcaactgg ataatatgtt tttagataag
    24481 ctgtctcata tgctaaaacc ccatatgcag ctgcatgtga tttattaaat gcatatctag
    24541 cgaagtctat catatcatca aatattttat tagctatttc ctcttctaca ccatttctga
    24601 tacaccctgc aatttctata ttaccatctt catcaaattt accatgaata aaatattgtc
    24661 tttcttcttc catcacgtcc atctttttct tactcatggc tcttctaact aggtcacttc
    24721 gtccgtaact ataaccacct aaatctctaa cgacctgcat aacctgctct tgatatacca
    24781 aacaaccata tgtaacttcc attataggct ttaatttttc atgtagatat gttacatctt
    24841 ttgggttgct tttatttttt atatatgcag gtatagaatc cattggacct ggtctaaata
    24901 gggatattcc cgctacaata tcttcaaaat tgtctggttt tagctgtttc ataaaacttc
    24961 tcatgccagc actctccaat tgaaacaccc ctagtgtatt tcctgatgaa agcatttcat
    25021 atactttcgg gtcgtcataa tccatctttg aaaagtctat tttttctgtg taatttttta
    25081 taactctatt tttttctatt aaatcaagag tatctcttat aactgtaaga gttcttagcc
    25141 caagaaaatc catctttaaa agcccaagtt cttcaagtgt tgtcatagta aactgtgttg
    25201 tgattgcatc ttgatgtttg tacaaaggta catattcatc tacgggattt tttgatataa
    25261 caactcctgc tgcatgagta gaagcatgtc ttagcattcc ctctatctgt tttgatatat
    25321 ttattatttc ttttgtttcc gggtcttggt catacaattc tttaagatta ggatttgtat
    25381 ctagggcctt atctattgtc attcctaaag caaatggtat ttccttggct accttgtcaa
    25441 ctttattgta gcctatatcc aatacccttc caacatctct tattgcagct tttgcaccca
    25501 ttgttccaaa agttattatc tgggctacat ggtcatcacc atattttctc tttacataat
    25561 ctataacttc ttctcttctc tcataacaaa aatctatatc tatatctggc atggatatac
    25621 gttctggatt taaaaaacgc tcaaataata gagagtactt tattgggtct atatcagtta
    25681 ttttaagtgt atatgctact atcgaaccag ccgcactacc tctacctggt cctactataa
    25741 tattattttc tttagaaaaa tttataaaat cccaaacaat taaaaaatac tctacataac
    25801 ccattttttc aattacatct aattcataat ttaatctctc tagtatctca ttactaggat
    25861 tatcatatct ctcttttaat ccattaaaac acaactccct taaataagtt tctggtgtat
    25921 atccttctgg aacatcatac ttaggaagat gtatagtatt aaaatcaaac tccacattac
    25981 acatatcagc tattttcaca gtattgtcaa tagcctctaa tgcatatgga aataattctt
    26041 ccatttcttc tcttgatttt aaataaaact catcactacc aaatctcatt ctatttgggt
    26101 catttaatgt ttttcccatt tgaatacaca taagtacatc atgtattttc gaatcttctt
    26161 tatttacata gtgaacatca ttagtagcaa ctagtggtat accagtctct tttgataatt
    26221 ttactaaacc agagtttact tctttttgct caggtaaatt gtgatcttga atctccaaaa
    26281 agaaattttc ttctccaaat atgtctctgt agtcaagagc aaattgtttt gctttttcat
    26341 aatttctatt cattaaagca cgtgctacat ctcctgcaag gcatgcagaa agtgctataa
    26401 tccctttgtt atgtttttta agttcttcca tgtcaactct aggcttatag taaaaacctt
    26461 ctacgtatga agttgaaact atttttatta aatttttata tccttccata ttcttagcta
    26521 acagaactaa atgtccttga tatttgtcat agtttgggtc tttatctctc aatccacgtg
    26581 cagctgtata gacttcacac cctataattg gctttattcc ttcttttata gctgttttgt
    26641 agaaatctat aacaccaaac atacaaccat ggtcagttat agctattgag ctcatgttta
    26701 gttccttagc cctttttata agttttttca ccctcgaaaa cccatcaagt aaactatact
    26761 cagtatgtac atgaagatga gcaaaatctt tgttcattaa atcacctcct gctttataat
    26821 tttaatttta ccacaaatat atgtcctaaa acaaaaacag agacttttat ctcaaaatag
    26881 gaattaactt caacatatac cattatatgt tataaattaa tgcttattct gagattgtcc
    26941 cctcaattta ttgctatgat ttttttttca aatctgttgc aatttcttct atttttctaa
    27001 gtctatgatt gactccagat tttcctattg gtggttctaa catctctcca agttctttga
    27061 gagtcatatc ctcatactcg attctaatct tagctatctc ttgtaaattt tctggcaaac
    27121 tgctaattcc tatagtctct tgtatgagct taatattttc tacttgtctt actgctgcat
    27181 ttactgtttt agaaaggtta gctgtctcac aatttactat cctgtttaca ttatttctca
    27241 tttcttttac tattttagta ttttgaagac ttaaaagagc gtggtgtcct cctataatac
    27301 tcaacaaatc tgatatttgt tcactctctt tgatataaac aacgtgatta ttttttctag
    27361 ccacaatttt actattaaaa cctaaagagt ttataagttc ttttagagaa tctgcaaact
    27421 cctcattatt agttacaaat tccatatgat agttcttttc tgggtcgctt atagaacctc
    27481 caccaagaaa agctcctctt atgaaagctc tcttacatcc ttcatgctta attaagttct
    27541 ctgaaatttt gtttatagta aaaaatcctt ctttatcttc aagtatacct aactttttta
    27601 ataacatttc tgaacccatt tcgcttgtta ccattaatac ataactgtta tttctcttta
    27661 gcatttgatt cttatttact gagattgtag tattgatatt aaaattcttt tttaataatt
    27721 taaatacttt acgggctatt gaatttaact ctgttgttat ctttagattt agcttcttat
    27781 atccaacaat ttgtatactt ccccctgatt taactagagc tgacaattca gctatattac
    27841 aacattcatt cccagatata actcttgcta gttcattttt ggtttcagtt gaaaaagaca
    27901 tatctatcac ctattaacta atttttcttt aacctttttt ttatttcttg taaggcaacc
    27961 tttattatct ccttttcatc atcataccta gctaagtcta ctactctatc taggtggata
    28021 aatttagcat caataaagcc ttcctctttt tgtggaatag tatccatatt ctttgcttgc
    28081 atcaaatacc aaaaaactgt tttatgaact gctctatttt catcccagtt ttctttgaat
    28141 gtatagtgta tttcacctaa atatttcaat atatctgctt ttactccagt ttcttcactt
    28201 acttcacgta atgctgcctc ttggttattt tccccttctt ctacttttcc cttgggtaac
    28261 acccaatctc cattgaactt ccttagtaaa agaatcgtat tgccaaacag gactacacca
    28321 ccagcactaa cctcttctct catcgatgtt cactccttaa aactaacata atgataagga
    28381 tttctacaca attttttaaa atcctttttt actaactttt gttgtaatta tgatttaggg
    28441 ctaactcaat aactatgtta gatatatact ttgcatcatg tcttatataa ttatttttta
    28501 tttcaattaa attcttctct acagttttta tacccatctc ttttaattta tctttttgct
    28561 ttttatctaa caagacttgc tctgctccat cttgtttata taagtcaagc atttcttctg
    28621 gcaatatctc attatttgcg attacataat ctattaaatt ctcatcagtg tgttttacta
    28681 tagcattaac atgctctaat acattgtacc cttctgtctc acctggttga gtcattatat
    28741 ttgctatata tacttttgga gctacagacg atttaatagc gtctacaatc ccctctacaa
    28801 gtaaattggg tattatactt gtatataaac ttccaggacc cataattata atgtcagcat
    28861 cataaataga tgcaataaca tcctttaatg gttttacatc ttttggattt aaaaaaattt
    28921 tatcaataga gcttttttga atttttgact cttcaggtat actagattct ccatttatta
    28981 tattgccatt ttctaatttg gcaactaaat ttacatcttc caaagttact ggtaaaactc
    29041 ttcctgttat agcaaaaatt tcacttaatt tatatacagc tttttcaaag tttccataca
    29101 acccattcat agcagccaaa aacaaatttc caaaactctg accttttaaa agcccttctg
    29161 taaacctata ttgcataact tcattcattg ttggctctat atttgcaagt gctaataaac
    29221 agtttcttat gtctcctggt gggagcattc ctaagtcttc tctcaataca cctgaacctc
    29281 caccatcatc agcaacagtt acaattgcag ttatattttg cgtagtgtgt ttaagacctc
    29341 ttaaaaatac cgattgacca gtacctccac ctatcacaac cacatttggt tctctatttt
    29401 caacaataga cctttgaaat agttttactt tttcattttt tgctttttta tatataaata
    29461 acgatacaat taatgcaatc caacctaaaa ttcctacaat aataagtaat ttagccatgt
    29521 ttgcctccat tatcgcaaca tagagtctct atgtttcttt acaactctat atcccttatt
    29581 agataaatcc tccgctataa gatttgttat agtaactgac ctatgtcttc cacctgtgca
    29641 tccaacacca attactagat gttgttttcc ttcctctata tattgaggta ctaaaaaatg
    29701 aatcatatct aaaagtttca cataaaattc ttcactgatt ttagaattca taacataatc
    29761 tctaacttct ttatcatcac cagttttagc tcttaactct tcaacataat agggatttgg
    29821 aagaaatctt acatcaaata ctaaatctgc atcagcaagt ataccatgtt taaatccaaa
    29881 agatactaca gaaatagtta ggtttggatt atcttcacct gaagaatata ttttctttat
    29941 ttcttcctta aggtctttag gtttcatgtt agttgtatca attatacatg tagataactc
    30001 ctttaaaggt tccattatct ttctttccat ttttatacct tctggaatct gcatatcttt
    30061 tgccaatgga tgatttcttc ttgtcatttt atatctcttt aacaatacat catcattaca
    30121 atccaaatat accatttcat attcataatt ttctttttct aaataattta agctttcatg
    30181 aagcgcttca aaaaactttc tccctcttat atctatccct agagcaactt tatcaatact
    30241 tgaatttggt tgataacata attctgcaaa tttagttatc aaagcaggag gtagattatc
    30301 tacacaataa aatcccatat cctctaaagc tctcattgtc tcactttttc cagatcctga
    30361 aagacctgta actataacaa atttcattgc taactcctcc cttttatctt atgtttatta
    30421 tattataatg ttttctacta acgatatatc cagattagag tcagatatag tcttcatagc
    30481 tagttcaaaa tcaccaactc tttctggaca atgtgcatct gagcctatta taaatttatt
    30541 ttctagccct tttattttat ttaattgatt tacattcaaa aaagaatggc tactatttat
    30601 ctcaagtcta gtatttgtct tttttgctgt ctttgctact tcttctatat acacatcgcc
    30661 tttatctcct ggatgagtaa ttataaaaat atcattatta cttatcgcat ttattacagc
    30721 ttttgtatta tattcttttg ctttttcagt tttaaaaata tagttattac aatgatttaa
    30781 taagcttctt aatgatgttg gagtagaacc aaaatgatat ccagccataa tataatcaaa
    30841 atctctaata aatttatcat ccatatctat atttccttta tcatctaata tgttacactc
    30901 catacctaaa agtatattta tatcagtgta cttatcattt aaataatcta cttcttctct
    30961 cattttgaga atatcttttt tctttacacc atatgcaata tgtttataac tatggtcaga
    31021 tattcctatt gtttttattc ctttagaaat ggctaccttt acattatcct caatagtccc
    31081 tttgccatga ctatataatg tatgtgtatg ataatcagcc agtattttca tttgttaatc
    31141 ctctccaagt attttgactt cttcttccaa cataacccca aacttggcat atacagcact
    31201 ttttataaca tatattaaat ccaaaatatc tttagcactt gcttctcctt gattgactac
    31261 aaatccacaa tgcttctcag atacttgtgc tcctcttaaa gtaagacctc tcaatccagt
    31321 ttcttctatt aatttacctg caaaatatcc tgttggtctc ttaaatgtac ttcctgcact
    31381 agcaaaattt aatggctgtt tagttattct tctcgtcgct aaatcatcca ttaaactttt
    31441 tatttcatca taatttcctt cttgtaattc caattctgct gataaagcta tatatccgtt
    31501 tttcgatatt atactttttc tatacccaaa ttccatctgt tcatttgata attcaaatat
    31561 attgccttcc atatccatca gtcttactga cttcactatg tccttcattt caccaccata
    31621 tgctccagca ttcatggcta atgctcctcc caatgtgcca ggtattcctg atgcaaactc
    31681 aaaccctttt aattcttgct tttggagagc tttccctaaa actgataata aagctcctga
    31741 ctgagcagtc atcctagtat catttatctc ataagaattg aaattatcag ctagttctat
    31801 taccactcct cttattccac catctttaat taaaatgttt gaaccatttc caattattaa
    31861 atacggaata gattcttctt ttaccaactt aagtacattt tttaattgtt cttcagttct
    31921 aggtctaact aatatatctg ctggtcctcc taccctaaat gaaatatgtt ttttcatagg
    31981 ttcatcgact tttatatcct ctttacttaa tatatttaat aaatttatat atatatcttg
    32041 attattcatt gtatttcctc ccaaattata ctgaacttta gctgtcatta ttatttaaat
    32101 tacattagta tatagtatta actatattca ataatatatg attttgctta tcatatggat
    32161 tttatatttt aagcttctta attacaaact atattcaata aatatataat aaagtaaaaa
    32221 aaaaaaaata acaacatata aaagaatata aataatttaa aaaaagcatc aatatcactg
    32281 atatcaatgc tttaaaaggc aatttaattc gtattattta tctaactatt tttgacatag
    32341 caaagataat aaatccctcc aactactatg gaacctccaa cgatatttcc aagcgttaca
    32401 aataccaaat tagttataaa ttgaaccata gttatatttg ctcctaatgc cattcccatt
    32461 ggtataaaaa acatgtttgc aacactatgt tggaatccac ataatacaaa tagcattatt
    32521 actgtccaac atgaagccaa ttttgacatt atatcttgag atgctgtagc catccataca
    32581 gctaaaacga ctagaatatt acaaagtatt gcttttaaaa acattgatgc tatagttgaa
    32641 ttaaccttag cttctgccac agctatagct tttgtagctg catctcctgt caatgtagaa
    32701 gaataaaaca caagtgcaac tagcaaaatt gaacctacta aatttgcaaa ataaaccagt
    32761 ccccagtttc ttaacatttt tcctaatgtt atttttttgt ccataagtgc aatagccatt
    32821 aaggtgtttc cagtgaataa ttctgctcct gccactacta caagcataag acctactgga
    32881 aagaccatag cacttaaaaa gctagctacc cctgggtcta tcttgccaaa agtttgacta
    32941 actatcatac tccccaatgc cccaaaacct ataaatgctc ctccaaataa acctaataga
    33001 attgcgtttt gtgtcgaaat atttgctttt ttaatgcctg cacttatagt tgcttgtgca
    33061 gtttccccag gtgtcaagaa tcctttactc ataaatcctt cccccttaaa aattatactt
    33121 tcccttgtct ttttatggca ataattatat aataacaata tgatatataa taaattttat
    33181 taacataaaa aatatactat atacttatat at
//
LOCUS       DAMJWS010000042        31609 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMJWS010000042 DAMJWS010000000
VERSION     DAMJWS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.042"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            177..833
                     /locus_tag="PC399_002064"
     CDS             177..833
                     /locus_tag="PC399_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592922.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1341..2612)
                     /gene="sleC"
                     /locus_tag="PC399_002065"
     CDS             complement(1341..2612)
                     /gene="sleC"
                     /locus_tag="PC399_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF2592923.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2857..3573
                     /locus_tag="PC399_002066"
     CDS             2857..3573
                     /locus_tag="PC399_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF2592924.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3652..4962
                     /locus_tag="PC399_002067"
     CDS             3652..4962
                     /locus_tag="PC399_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF2592925.1"
                     /translation="MTELPNEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5062..5598
                     /gene="lepB"
                     /locus_tag="PC399_002068"
     CDS             5062..5598
                     /gene="lepB"
                     /locus_tag="PC399_002068"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2592926.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5764..6300
                     /gene="lepB"
                     /locus_tag="PC399_002069"
     CDS             5764..6300
                     /gene="lepB"
                     /locus_tag="PC399_002069"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2592927.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6310..7065
                     /locus_tag="PC399_002070"
     CDS             6310..7065
                     /locus_tag="PC399_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF2592928.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            7139..8806
                     /locus_tag="PC399_002071"
     CDS             7139..8806
                     /locus_tag="PC399_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF2592929.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            9339..10268
                     /gene="glsA"
                     /locus_tag="PC399_002072"
     CDS             9339..10268
                     /gene="glsA"
                     /locus_tag="PC399_002072"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF2592930.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            10366..10929
                     /locus_tag="PC399_002073"
     CDS             10366..10929
                     /locus_tag="PC399_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2592931.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            11417..12253
                     /locus_tag="PC399_002074"
     CDS             11417..12253
                     /locus_tag="PC399_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF2592932.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKKELKMIKEILS
                     SK"
     gene            12537..13532
                     /locus_tag="PC399_002075"
     CDS             12537..13532
                     /locus_tag="PC399_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2592933.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(13789..14193)
                     /locus_tag="PC399_002076"
     CDS             complement(13789..14193)
                     /locus_tag="PC399_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2592934.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            15034..15948
                     /gene="queG"
                     /locus_tag="PC399_002077"
     CDS             15034..15948
                     /gene="queG"
                     /locus_tag="PC399_002077"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF2592935.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKNRQQKGYLTG
                     MEEPIIESRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            16054..18291
                     /locus_tag="PC399_002078"
     CDS             16054..18291
                     /locus_tag="PC399_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2592936.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTKQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            18489..19706
                     /gene="nth"
                     /locus_tag="PC399_002079"
     CDS             18489..19706
                     /gene="nth"
                     /locus_tag="PC399_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF2592937.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKKEINKKGIDKKDIIEKKEINKKEMDKKDIIEKEEINK
                     KGMNKKDIIEKEEINEKEIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKG
                     ETEKKTKKSSSNKATQKTSKETKSKFKIKNESANEKDVNKILDELEKLYPDAKCELNY
                     GTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKS
                     KSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVF
                     RVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIK
                     EDCNYYKELNETK"
     gene            19900..20370
                     /gene="trmL"
                     /locus_tag="PC399_002080"
     CDS             19900..20370
                     /gene="trmL"
                     /locus_tag="PC399_002080"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF2592938.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            20406..21254
                     /locus_tag="PC399_002081"
     CDS             20406..21254
                     /locus_tag="PC399_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2592939.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKKLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            21559..22563
                     /locus_tag="PC399_002082"
     CDS             21559..22563
                     /locus_tag="PC399_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2592940.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            22689..23801
                     /locus_tag="PC399_002083"
     CDS             22689..23801
                     /locus_tag="PC399_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2592941.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATEYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            24291..25052
                     /locus_tag="PC399_002084"
     CDS             24291..25052
                     /locus_tag="PC399_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592942.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            25055..25873
                     /locus_tag="PC399_002085"
     CDS             25055..25873
                     /locus_tag="PC399_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF2592943.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            26053..26655
                     /locus_tag="PC399_002086"
     CDS             26053..26655
                     /locus_tag="PC399_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF2592944.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    27114..27351
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            27415..29334
                     /gene="thrS"
                     /locus_tag="PC399_002087"
     CDS             27415..29334
                     /gene="thrS"
                     /locus_tag="PC399_002087"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF2592945.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            30102..31193
                     /locus_tag="PC399_002088"
     CDS             30102..31193
                     /locus_tag="PC399_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2592946.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT        12613 a         3015 c         5814 g        10167 t
ORIGIN      
        1 gataatggtg gatagagtag taagaaaata aatagataaa gggatagaat aaatttaaat
       61 atggaaaatg aatatctaat aataagtgat taataaataa ttgtagatag attgtaaatt
      121 aaataaatct ttaatagaaa ggagaactat ttatatatta aatagtattt ataaatatga
      181 aaaaaggcga ttttatatgg agtggagttc ttatttgtat tattgctgtc ttagcaatac
      241 caagtagtag agaagtattt atggaagcaa caggtgcaca tccatatatt ggaggttttt
      301 taaagtttgc tgtattagct acaatgggag atttacttgg tgctagaatt ttgtataagg
      361 attggaaaat accagtagga gtattttata gagcaatagt ttggggaata ataggtgtag
      421 ttaccacata tggaatgtca atctactcat caggtgtagg tcaagcacaa gtggatggta
      481 tgcttccatt tgagggaata gcttttgcaa atgctctatt taagagtgca actatgaata
      541 tactaatgtc accaactgta tttttattcc ataaggttat ggactgtttt atagataaaa
      601 aatatgaggt tggaaagggg attaaggtaa aacttaaaga tgtggtaaat attgtggact
      661 ggaatgctta ccttggtttt agtgttttaa aaactatacc atttttctgg ataccatgtc
      721 atacaattgt attcttattc cctgctgagt atagagtaat tgcttctgca tttgcatcta
      781 tagctttagg tctaatattg gctttagcta ataagtctaa agttgcagtt taggttcact
      841 gatgaagata gtagtattat ttaaattagg aataactttt taaattaata ttatttgtat
      901 ttataataaa taataattta tataagaagt aatattctaa gtgatggata gtgttttaaa
      961 taataagaag taatactcta agtgatagat agtgttttaa ataataagga gtaatactct
     1021 aagtgagaag tagcatttta aataataagt gagattttaa ataaaaagta ggtttttcgt
     1081 tagaatagca ttttattaaa cttaaaaata aatataatat tttaaagaaa tctaagttgc
     1141 taagcactgt ttttaagaag cttaaaatcc cccatttaaa gttttaagat tctaaaaaaa
     1201 caagttataa ctaaaaaatg gctgttaaga ctacaatact tataagtaat acttatggta
     1261 tatattagtt cataacagcc atttttattt taaattaata attttattca ataatttgag
     1321 tttatgttct aaatcaagct ttaaattaaa ggatttaaag aagctattct agttgtagct
     1381 acataaactc tggatatttc ataccatgtt ttaaaatcta ctactccgct ttgaggtaaa
     1441 ccaaagattc cttggaatat cttaacagcg ttttctgtat ctgttccata gataccatct
     1501 tctataactt ttggtacagc tggataagaa ttagatatag catttaactg attttgtatg
     1561 gttctaacat actgtccaga agaaccaact tgtagtgtag taccagggaa tgacactggt
     1621 acaccactta ctataggcgc tctttcaaat actatctcat ctccatagaa atatcttagt
     1681 atactctcat agtcatatcc ttggtcacct aagtctttac tgccccattg agtcatttgg
     1741 tcaggacatt gggatttttg tccatcacaa tattgtgcta atagtggctg tcttgatgta
     1801 ggaggtcttt ttataaaagt attaaaaata gcatctacaa ctacgttaat tggttcaaat
     1861 aagtttctat tatttataaa cttatggtcg tatgctgttg ttgaagttat tgtaaagtta
     1921 tatcctttat ttctatacca ttcagtaaag actctattta aagtaaatga tattatagct
     1981 attacatttg cataaattgt ctgttcaggc caagttgaat atatttcaga tgatgctatg
     2041 tttttaatgt actctttaaa tggaatccaa tagtttggag ctgatgaatc ttctggcaaa
     2101 ccatcatgaa ctactataaa ttcaggcact acagggttat ctaatacaac gaaaccagta
     2161 ggaggtggta atggttttaa gttactttct ggaatcttag gaggataagt accatataga
     2221 gtatgttctc ctatgtcaaa aatcaactca ctttgtcttg agaaagaacg cttacttctt
     2281 gttctaggag acatgggaac tccttgtctt gcttcaattg tagctaacaa ctgtgttcct
     2341 tctatgacaa ctgtctcata tccatctgct atggcttcca ctatatactg gctataaggt
     2401 ctaacatcag atggttgtag agagtaatct acatctggtg ctggtaatac taaaccagtc
     2461 acttgaccag attcattact tcttaggttt tggtataaag ttgatgagct ttggctacca
     2521 ttagacattg aatatatgtt tactactgca ttttgaatag gtctattgtt agtagcatca
     2581 attatgctta ctgttaagaa accatcttgc atcaaatcac cctttcttta aatgaattta
     2641 gttcccttat atttcaagat atgaatatag cttaaaattt gttaaataat atagatataa
     2701 atttaaattt ttttgagtat ttgagtatat ttgtatttaa ataatttaaa ataatttaat
     2761 gtatatttta gtatgtattt aaaacacgaa taagttaaaa ataagtagat gatataaatc
     2821 ataaataaat caaaaatttt gaaaggaaaa taatctatga agctagattt atttttatta
     2881 gcaatagttc ctatattgat aggaatgttt tggataaggt ctaaagatag atattgtaga
     2941 gaacccctaa tccatctaat taaatttttt ttaataggtg cttttctaag tgttataatt
     3001 atacttttag aaaatttact gatgaagttt aatgtatttg aagggtattc agaactaata
     3061 tatgtatcat ttgtagttgc aggacttgta gaagaaggtg taaaagctct gatactgatt
     3121 cctgccctaa ttaaagaaaa acactttact gaaaagttag atggtattat ttactctgta
     3181 tttttagctt taggatttgc aacaattgaa aatatggttt atattttttc tgaaagtaga
     3241 aatctagcac tgcaagttgg tattaatagg gcagttatct ctataccagc tcatgtaatg
     3301 tttgcaatta ctatgggcta ctatatttct aagtataaat ttgagggaaa taaaaataaa
     3361 agacgtgagt atttgtttat ggcagtgtta attccaattt tattacatgg agtttttgat
     3421 ttcatattga tgatagaata tagatgggct attatattac ttatagttta tgtaataatt
     3481 ttgtggaaga taaatcttga taaacttgag aaatatatga atcactctaa aaaagtgttt
     3541 ttcggtaatc ttaggaaaaa gaagaagaaa taaaatttaa aggaaagaca taaataatta
     3601 aagtataata aatgacacac aagtaaagca gattgaaggg ggattataga tttgacagaa
     3661 ttaccaaatg aatttttaga agatattgaa gaaatactaa aagatgaata tgatgatttt
     3721 ataaaaagtt atgaggaaag taagactact gggcttagaa taaatacttt aaaaattgac
     3781 aagaaaaaat tatttgattt ggagttgttt gatttgaatc aaataccatg ggcagaagaa
     3841 ggtttttatt atgatgataa aattgacaga cctggtagaa atccactgca tgaggctgga
     3901 gcatactatt tgcaagagcc ttctgcaatg agtgtaatac caaaggcaga tattaaagag
     3961 ggtgaaaaag ttctcgatat gtgtgctgct cctggtggaa aatctacata tatactatct
     4021 aagctaaacg atacaggttt attggtttct aatgaaatta atgctagtag aattaaggct
     4081 ctaggggaaa atttagagcg atttggagcg agaaattgta ttataacaaa caccgattct
     4141 aatagcttaa gaaagacgtt taatgggtat tttgataagg ttattataga tgcaccttgt
     4201 tctgggcaag gtatgtttag gaaggatgag gttgccatat cagattggag ttatgcaaag
     4261 gtagtagaat gtcaatccat ccaaaaagag attataagag atggatataa aatgttaaag
     4321 aaggatggca ttttaattta ctcaacttgt actttttcaa gggaggaaaa tgagcatgtt
     4381 ataaatgagt ttatcagtga atatccacat gcagaactta ttgaaatgga aagattatgg
     4441 cctcataaga taaagggaga aggtcatttt gttgccaaaa taaaaaaatt agaagaagaa
     4501 gaatgtaatg tcaaagaatt aaaaataaaa aatttagata aagaaataaa ggattatagg
     4561 gagtttgaaa agaaattttt aaaggttgat ttttatagta ataaaaaaaa taaattttat
     4621 ttaagaggag aaaatttgta cctacttcct gaaatatatc cagatacgaa aaaactaaaa
     4681 gtattgagat atggtttgca tttaggggtt ttaaagaaga atagatttga gccttctcat
     4741 gcactatctc attacttaaa agttgaagat gtaaaaaatg ttgaaaactt cagtgtacag
     4801 agtgaatcaa ttcttaaata tttaaaaggg gatgttgtaa attctaatga tagtagagga
     4861 tgggttttgg tttcggttga aggaatacct ttaggttggg gaaaagaatc ttctggtgta
     4921 attaagaatc attatcctaa aggtcttaga aaagtatttt aaactattag aaaaaagaag
     4981 tataatattt atattgtagg tttatggtag attattttgc tgtagattga cttaatattt
     5041 aacacagagg agacgatttt agtgggtgaa gcagttaaaa aagaagttgt agaatggata
     5101 aaagtgattg tcatagctct tgttttggca tttgcaataa ctcgttttat agtgccaaca
     5161 atagtcaaag gagaatcaat gtatcctaca ttagttgaac gtgattattt gatagttaac
     5221 agaattgcgt acaaggtagg agagccaaaa tacaaagata taatagtatt caaaaccgac
     5281 ttaacagagg aaaatggaaa gaaaaaagat ttagtaaaaa gagttatagg tgttcctggt
     5341 gaccatgtaa aaatacaaga ctccaaggta tatgtaaatg ataagttgtt agatgagact
     5401 tcctatatac ataataatcg tactgatgga gatattgata tcgtagttcc agaaggaaaa
     5461 ttatttgcaa tgggagataa tagagaaaaa agtttagata gtagatacga tgaggttgga
     5521 ttggtcgacg agcataccat tttaggaaag gttctagtca gattgtatcc attttctaag
     5581 ataggaacta ttgactaaac atataaaatt tggagcaatc gctgattagc tgaaatcacc
     5641 agaccataca caatagttgt ctatataaaa aagacaacac cttatttagt agacttatct
     5701 aaaagatagt gtttaagaga cattatcaat aaagataata taactataaa ggagaatttg
     5761 tgaatgaatg aaactattaa agaagagatt gtagagtgga taaaaataat tattactgca
     5821 cttttttttg catttattat aactcgtttt ataaaaccaa cattagtaaa tggagaatca
     5881 atgtacccaa cacttaaatc acatgattat ttggtagcaa acaggatgac atataagtta
     5941 tcagaaccaa aatgtggaga tataatgata tttaagactg atttattaca agagaatgga
     6001 aggaaaaaag agcttgtaaa aagggttata ggtgttcctg gtgaccatct aaaaattaag
     6061 gattctaagg tttatataaa tggtaagtta ttaaatgaag tttcatatat acatgataat
     6121 tatactgaag gcgatattga tatggtgatt cctaagggaa aagtatttgc gatgggagac
     6181 aatagagaag ttagtttaga cagtagatat aaagaagtgg gattagtaga tgaagaaaat
     6241 attaaaggaa aagttatttt aagagtattt ccttttacag atataggtat ttttgagtag
     6301 gtgatttgta tggcagttgg tatagcgtcc ttattgttta atattgagga ggctttaaac
     6361 atttgtgaga gtataaagca aataactcat ttagagattg gaattgacaa tatatctgaa
     6421 tgtagtgagt tatgtaaata taaagagaga atttctaagt taggattatc tatagggata
     6481 cacttgccta tggaattaaa tacttgcgaa aacatagagt atatacgaaa ttcatggata
     6541 aattttattg agaagataga atttgaacta aaaggatttg atttaaggta ttttaacctt
     6601 catttgggat atgttatgac aaatagagtt agaaagaata gagacaaata tttaggaaat
     6661 agtgtggatt ttttagataa attaaataca aattcatatg tatgtataga gaatacttat
     6721 tcaaaaggtg gggatttttc aaatattgga aatatatctt atgattttga atatatattt
     6781 aagaggatta aaaattctaa gatttgtttt tgttacgata ctggacactg tttaattgat
     6841 gaggatgctt atgtaaagaa cttaaaggat aaaataagac tgatacactt aagtgataat
     6901 gatggtatca atgatacaca tgttggtata ggaagaggaa ttttatctga agaggggatt
     6961 aaagaggtct taacattaga tgcagaatat ttagtattag aaataaatta cgaagacatt
     7021 gaagatacta taagtaaatt gaataatatt gtaggagagg gttaaatacc tctctttatc
     7081 ttgttgatat gcctcattaa ttaaaattta tattcatcaa aaggaaggag atgttgaagt
     7141 gaaaaaaata aacttaaaaa tagaaggaaa agataaggaa tattcattag aagaaaattc
     7201 ccctggaatc agattgggag atatagcaaa agaattttgt gatgagcata agggttatat
     7261 aacacttgca gttgtagaca ataaactaaa agagctaaat tgtagagtta agaaggattg
     7321 tgagataaat tttttagata ctactaatga agatggagag agagtttact ttagagtcat
     7381 gtcatttata tttgtaatgg catgtagaga aatcttttgg gatagcagag ttacaataga
     7441 acattcacta tcagatgggc tatattgtga agttcatata gatagaaagc ttaaagaagc
     7501 agatgttgaa aatataaaaa acaagatgaa agagatagta aacaacgatt atgttataga
     7561 aaaaattgaa gttacaagaa atgaagctat ttctatcttt gaaaataatg aaatgtatga
     7621 aaaggcagag cttttgaaat acaaagagta tgatagtgct aaagtatata agtgtagaaa
     7681 ctatatagac catttttatg gttatatgtt gccatcaact ggttatataa aatcatttga
     7741 tattaagctt tataatggag gcgttataat tttaggacct agtgaagaag ataagactct
     7801 tcctatgaaa tttgtacctc aacctaagct atcaagtgta tattatgaag cagaagaatg
     7861 gtcaagactt atgggaattg acaaggtagt atctcttaat aaaataatag aaaacaatga
     7921 atatggagat ataataagga catttgaagc attgcatgaa aagaagctat ctcaaatagc
     7981 ggatatgata aaggatggca ataagagagt agttttaata gcagctccat cttcatctgg
     8041 aaaaacaagt tttgctcaca gattatcaat acatcttaga gtaaataatt taaatcctat
     8101 ttcaatttct ttagatgatt attttataga tagaaaacat actcctttag atgaatatgg
     8161 aaattatgat tttgaatcaa tttatgcaat agatttagag aagtttaact tagacttaaa
     8221 gaaactttta gctggtgaag aaattgatga tataagattc aattttaaag aaggtaagag
     8281 agaatacact ggtaaaaaga taaagataga ttcaaatcag ccaattatat tagaaggtat
     8341 acatgggcta aatcctattt taacatcatc tataccagat gaagataaat ttaagatata
     8401 cataagtgcc cttactcaaa taaatttgga tgaccataat cgaatcccta ctgctgattt
     8461 gagaatgata agaagaattg ttagagacta taattttaga ggatatagtg ctgataatac
     8521 tattttacaa tgggcatcag ttagaagagg agaaaagaag aatatattcc cgtaccaaga
     8581 ggaggcagat gctatattta actctgcttg tgtatatgaa ttggcagttt taaaacgatt
     8641 tgcgaaacct ttgttggaag agataaagga agataatcct gcacatattg aagctactag
     8701 attactaaag tttttgcagt attttgttga attggacgat acttctgaca taccaggtat
     8761 ttcaattctt agggaattta ttggaggaag taaaatagtt gattaatgtg tgtattatag
     8821 attgttttat gaagattaaa tggcatagca agtagtgtta ttggtttatg ttaagtatat
     8881 taagtgtaaa gtggagaatt aaattgatac agattttaaa gtttgagttg atatataaaa
     8941 aggcttgttt tatacttttg tatgaagcaa gctttttatt ttaaatttta cttttttgtt
     9001 taatattttg ttagtttatg tgattatttt ttaaaagagt aaaagagtaa aagagtaaaa
     9061 gagtaaaaga gtaaaagagt aaaaggtgtt gggttagaaa tttttggtgt tataaaaatt
     9121 taaattactg gattattaga ttgctggatt gctaaattac taggttgtta aattattgaa
     9181 ttactaaatt attaagctct ttaaactatt taaattgttt gaaaaatgaa ttgattcttg
     9241 tagaaaataa ttttaaagta taattttttt taacattgtt ttattatagt ataaacttgt
     9301 gctaaaatag aatatataac ataatataag aggtgcatat gaatacgtta gatgagacat
     9361 tattaaaaga aattataagt tctaataaga agtatactaa ttatggacaa gtcgctagtt
     9421 acatcccaga attaaaaaac gctagaagaa atgatttagg aatttgtatc atagatagcg
     9481 aaaataatct ttatagtgct ggaaactgta gcacaaagtt tactatacaa agtatttcaa
     9541 aaccaattgt gcttgctatg gcacttatgg ataatgattg ggaagacgtt ttttcaaacg
     9601 ttggaatgga gccaagtggg gacccattta attcaataat gaaacttgaa attaatgaca
     9661 caaaaaaacc ctgtaatcca atgataaatg caggggctat agtaaccact tcccttataa
     9721 atgggagttg tttagaagaa aaagaagaaa gaatgcttag tttctttaga aagttagcaa
     9781 aaaatgataa tataggaata aactatgatg tctataagtc tgaaaaaatg actggagata
     9841 gaaacagagc tatggcttac ttattgaaaa gtgatggatt tatcagaggg aatgtagaag
     9901 atgttctaga cctttatttt aaacaatgct caattgagat agattctgta gatttagcga
     9961 gaattggaat aaatcttgca aattatggag tagatataga aaatggtgag catttaatga
    10021 gtgaaatggt ttctaggatt gtcaagacat ttatgatgac ttgtggaatg tatgatgctt
    10081 ctggggaatt tgctattaaa gttggcattc ctgcaaaatc tggagttggg ggtggtataa
    10141 tggcttctgt gccgggacgc atggggattg gtgtttatgg acctgcgctt gacaaaaaag
    10201 gtaacagcgt cgcaggagtt aaagttttag aggagttgtc aaataaactc aaattaaata
    10261 ttttttaaaa tattaagaaa ggattttttt gacaacaaca tataatttaa tatataatta
    10321 aattgtaaag aaatatgaat taaataaatt ttttaggagg ggtcaatgaa agaggtattg
    10381 gtattacgag atttggagtg cataaaggct attgcgcatc cacgtagaat agatattcta
    10441 aaagcatttg acaaatcgcc attgtcagca aaacaattat ctcaattatt ggaagaacct
    10501 catgcaaaaa taaactacca tataaaaatg ttatacaaag ttggtatttt agaattagtt
    10561 gaggaaaaaa taaagtcggg gattgtggaa aagtactatt atccaagtgc aaaaaatgtg
    10621 gttataggga ataggatact taatttttct ttagacaatg gggaagaaaa agaagaattg
    10681 tacatatcaa aatttgaaaa tatgagtgaa gttttttata aggcagcaga ggaagatgtt
    10741 ttagaaaatg aaaatatagt tgattatcat gatatttctc ttactcatga tgaattagtt
    10801 gaacttagtg acactatgaa gtcaaagata gatgaaattt taaataaaag acaacacaat
    10861 gttgaaggtt ctaagtatga tatagctatg gttatagttc ctactcttga ggaggaatgt
    10921 cctagttaga gaattaatta tggattaatt aaagatataa tattagagat tgaacatcaa
    10981 aatttactta ataatcaaaa aatagttttt ggtatgggta aaacaatgat ttttaatctc
    11041 tatttttttg tttttttata cctattgtat ttgtaaataa tatgtatagt gtaagatgta
    11101 tacactatct aagaagtgct tagataatat acaaagtatg taagattata atatttgaaa
    11161 ttgattaaaa tgggtataat taaagtgtaa atattaaaat taaaaattaa tataaattaa
    11221 atcaatgcta gaaatatgtt aaaagaggac tgagattagt tgggatagaa catcccttag
    11281 agcttacgaa tttttagtga aaaagtacta tcaaatagga atctccatct tcatatgttg
    11341 gctaaatgta gttaaggtgg agtagttcaa aattaaaatt gagtacaagt agtagtacaa
    11401 ataaaaggag attaatatga ttaaattagg aacacatctt tcaatagcaa aaggatttgc
    11461 taatgcggct gagacagctg tatatattgg tgcaaataca tttcaatttt ttagtagaaa
    11521 tccaagagga ggaaatgcta aagaatttga tgaaaaagat ataattaaat ttcaagaaat
    11581 tagaaaagaa aataattttg caccactgct tgcacatgca ccatatacta tgaatctggg
    11641 tggtacaaag gatgatgtat acgaatttgc aacaagggtg atagatgagg atataagaag
    11701 gatggactca ttaggaattg aatacatgtg ttttcatcct ggaagtcatg taggtggtgg
    11761 tgtagatttt ggcatagata gaatagtaaa aggattgaac aatgccataa agggtgatga
    11821 aaatataact atacttcttg aaactatgtc gggaaaggga actgaaatag gttttaaatt
    11881 tgagcaatta aaaagtataa tagacaaaat tgagcataaa gaaaaaatag gtgtgtgtct
    11941 tgatacatgt catatttttt cagctggtta tgatatagtg aatgatttag atggggtttt
    12001 ggaagaattt gataaggtta taggcataga aagattgaaa actgtccatc taaatgatag
    12061 tatgatgcca tttggagata aaaaagacag acatgctcct ataggggaag gaaagatagg
    12121 attaaaagca ttaattgatt ttatggaaca tcctagtcta aaacatttac catttttctt
    12181 agaaactcct tttgatgatg aaggtcacaa gaaagagctt aaaatgataa aagaaatact
    12241 atctagtaaa taaaccttta ttaaaaatat gtgaaatact tcaccctcaa cataattagg
    12301 gtggagatac aatcatgaaa tgtgataatt taaattaatg gataggaata acaaaaattg
    12361 tatacaaatt aactgggtat aaagtatgat tatattatga atagactgat ataaaatata
    12421 attatactat gaatagtatg taaatatatt gcaaagattc tcataatttg ttgtataatt
    12481 aaagatgaca tatttatatt atataaaaaa ttattaaaag ttaggtgggg aaagatatga
    12541 gaaatttagg caatactaac atgaaaataa agagagttgg ctttgggggc attccaatac
    12601 aaaggattac tcaagatgat actaacttag ttataaatga gttagaaaaa caaggaataa
    12661 actttattga tagtgcaaga gggtatacaa taagtgagga agcaatcgga attgccattg
    12721 aaggaaaaag agataaattt ttcttagcta ctaaaagtat gtccagagat tatgattcaa
    12781 tgaagagaga tgttgaaata agtttaaata attttaaaac tgattttata gatttatatc
    12841 aattccataa tgttaaagaa gaggagtatg ataatctttt taaagataaa atggcttata
    12901 gtgccctttt agaggcaaag gagcaaggta agataaaaca tattggtata actagtcaca
    12961 atttaaatac tatagaaaaa gctatagaag atggaaaatt tgacactata caattccctt
    13021 acaatatagt tgaaggacaa gcagatgaag tgtttaaaaa agctcatgaa aaaggaattg
    13081 ggataatagt aatgaaacct ttagctggag gtgctttaga caatgcaaca ctggctatta
    13141 aatatatact atctaaagat tacatagatg tagttatacc aggtatggag agtgttgaac
    13201 aggttagaca aaatgtcgct gtacttgaaa atcttgtgct agatgaaaaa gataataagg
    13261 aaattgaaga aataagaaat tctttaggta aaaaattctg tagaagatgc gaatattgta
    13321 tgccttgtgc tgtaggaata aatataccat tgagtttttt atgtgaaggt tattatacta
    13381 gatatggatt aaaagagtgg gctaaagaaa aatatgaggt tatggatgtt aaacctactg
    13441 agtgtattga ctgtgggctt tgcgagagta gatgtccata tgaacttcct ataagagaaa
    13501 tgcttaagac tgttgttgaa aagctaggat agattttatt tattgaaatc ggtgtattaa
    13561 atttactccg atttcttttt attagatgtg gtcttatata caaatattaa atttacatag
    13621 ttatagctat tatataaatt taatatttgt ttaatactta ttaaatattt acttaatgct
    13681 ttttaaaaac atttgtttga gtatagacat aacacaatta ttattgttgt attagtatag
    13741 tttcaaatac tggagtatca atataatgtc gaataacaca taaaatcttt atttttcaaa
    13801 aatattatag gatatatcgt atttagatac tggataaagt tcaatttcaa ccctagggtt
    13861 atctttatct ataaaatcaa ataaaagtac tggcttaagt tgtttatcat tttttattat
    13921 tccacttttt tcaataccat cacaaatact ttttggataa ttatgtaaat cccccattct
    13981 cttatttggg aagaataatt tcaagactgt tattagattt tcttcaacag tttccccttg
    14041 atactgttgg tttataaaac ctgcaatcat gttttcgtat gcaagatact tagaatattt
    14101 tccctttgaa ggcatccatg cttgaccatt tatgttatga agcttaaaat tagatttact
    14161 aataggtcta cctggaattg ttattttaat caaatttttc accatcctta tacagtattt
    14221 attataatct gtatatgatt taaaagcaaa ttgttattta aagtgttttt acagagcttg
    14281 tttgtaaaat tttccacttt agatgtagta atataagata agtattttgc ttacaaattt
    14341 taaaatttga tatatgatgg gcatattcat attagactat tataagacct tcataatatt
    14401 aataagtata agtttagtaa agagagtaaa aagaaatcca tataagtcaa gtaaagactt
    14461 aataaatggc aatgtaaatg ggatattaat tataagaaaa aatcaatctt tttgcttaac
    14521 tatagaaaga atgtcaaaat ataactaatg atatttgaag ttttagagtt tatattaaaa
    14581 ggtaagaagg ttaagtgggt aaaaaaggtt gaatagatta aaaaggctaa gtatctaaaa
    14641 gagttaagta ggtaaaaaag ttaaataagt aaatgaaaaa ggctaagtat ctaaaagagt
    14701 tagtaggtaa aaaagttaaa taagtaaata aaaaagatga agtatctaaa agagttagta
    14761 ggtaaaaaac taaataggta aaaagatgaa gtatctaaaa gagttagtag gtaaaaaatt
    14821 aaataggtaa aaagacgaag tacctaaaag agttagtagg taaaaaaatt aaatagataa
    14881 aaagacgaag tatctaaaag agttaagcag gtaagaaaat tgaatagcta aaaagattaa
    14941 gtatttaaaa gagttagtag ctagaaaagg ttaaaaagac taaaaaaatt aaataaattt
    15001 aaaaagtcaa ttagattaaa aaggaataat tttatggata ataaggaatt aaaagcattt
    15061 tgtaatttaa taggattaaa ctgtgtagga gtagcagggg tagataaata tgataattta
    15121 gaaaagatac ttaagaacag acaacaaaaa ggttatttaa ctggtatgga agaaccaata
    15181 attgagagca gaattaaccc aagacatata atgaaggatg ctaactctat aatagtctgt
    15241 gcatttccat atcatataaa tagacaagaa aagaataaaa attcaaattt atcaaaatat
    15301 tgtcatggaa aggattatca cattgtagta aaggactttt tacaacaaat ttgtgattat
    15361 atttctaaaa cagtaacaga ttttaaatac aagttatttg ctgataatgg accattagtt
    15421 gacaggtatt tagcttattt atcaggaata ggatattttg gaataaataa taatatcata
    15481 actgatgaat atggttctta cgtatttata gggtatatag taaataatta taaatttgaa
    15541 gaagataaac cactagaaaa aagttgtttt aaatgtggta agtgtgtaaa atattgtccg
    15601 ggaaatgcta tattgggaaa ttatgatatg aaccccaaaa aatgtttatc atatataact
    15661 caaaaaaaag gagatttgtc agagaaagaa aaagttgttt taaagaatgg caaaaaggta
    15721 tttggatgcg atatttgtca agaagtatgt ccacataatg ttgaaatacc aacaactcat
    15781 attttggaat ttaaagaaaa tataatagat tatttggatt atgatgagat acaaaatatt
    15841 tcaaataaag aatttaaaag gagatatgga gatagagctt ttagctggag aggtagaaat
    15901 ataataaaga gaaatatgga aataatttta gataagtatg atgactaata ataagagtct
    15961 gtttcatata ctttaatgtg tatttacttt aacacttgtt gtgtaaaagt catattatat
    16021 cattaaagtt aaatatgggg aggtggggga atattgaaat ataataatac atctgaaata
    16081 aatccactag attatatatt gggtcaagaa agagctgtag aagctatgga attagggttg
    16141 aaaataaata atccagcgta caacatatat atagctggtg aacctggaac tggtaaaagt
    16201 acttatgcct taaaggtttt aaatgagtat gcatccaaaa aaaatacgca taaagattgg
    16261 tgttatatat ataattttga aaaccctaga gagcctatta tagttgaatt agaaaagggg
    16321 tttggaagag aacttaagaa agatatggag aaattaattg aaagtctttt agaagatttt
    16381 aaaaatgcgt ttgaaagtga aaattttgaa atagaaaaaa ataaacttct ggatgaatat
    16441 gaaatagaaa aagatatgct tttgaagcaa attaaaaaat atggagaaga aaaaggattt
    16501 aaattaaaac agagtaaaat gggaatagtt tttatccctt tgaaagaaga ggaagatgaa
    16561 agtgatgagt ctgatgaaga gttttacaaa gctaaaagag aactagaaaa tatggcgata
    16621 caggtcgtat ataaaattag aaacttagaa gaaatagctg aaaaagcagt gttagagctt
    16681 gaagaagaga tagctaaact tgtagtagaa cctcatatca agagattgtg tgaaaaatat
    16741 gaaaattatg ataagattca aacttatctg gaaaatataa agaaagatat aatagaatat
    16801 atgtatttat tctatttaga tgaagaggag ttaaaagata agtatgataa agagcatttt
    16861 attaaatata aaattaacct atttgtagac aatggaagct caaagaataa agaatcagca
    16921 cctgttgtag tagagataaa cccaagtcca gcaaatctat ttggaaaggc agagtatgat
    16981 tatgcaaatg gaaatataaa gactgatttc acaaaactat tatcaggagc atttcatagg
    17041 gctaatggtg gttatttagt actttatgca gaccaattac taaaatatac aatgtcttgg
    17101 gaaattctta aaaagacttt gcagactaaa aaagtaatat tggaaacaca aacagcaatt
    17161 aaaccagaaa atatgccatt ggatgttaaa ttagtactga ttggaagcca ctatatatat
    17221 gatattttat atagatatga tgaagatttt gaaaaatatt ttaaagtatt tgtagacttt
    17281 gatagtgaaa tggataaaaa tgaagataat gaagaaggaa tagcaagatt tatagcatac
    17341 caatgtgata agaataattt aaggcatttt actaaatcag cagttgaaga ggttattaaa
    17401 tttagcacta gattatctgg tgatttagaa aaattatcaa ctaagtttaa taaaataatg
    17461 gaagttgtaa tagaaggaag tgcttatgca gagtttagaa attcggagta tacaaagcag
    17521 tgtgatgtta aaaaagcaat aagtgaaaag agaaaaagaa tcaatagagt ggaaacacat
    17581 atggatgaat caattgaaaa tggatttact ctaatagaaa cagagggaag aaaagtagga
    17641 gtgataaatg gtttgtcagt cttaagtaca ggagaatact cttttggtag agcttcaagg
    17701 attacagcga ccactagccc aggaagtaaa ggcatagtaa atatagaaag agaagtaaat
    17761 atgagtggct ctatacataa caaaggagta cttatattag gtggctatct ttctgagaat
    17821 tttgcacagg atttactatt gtcactaaat gcatatgttt gttttgaaca aaattatggt
    17881 ggaatagatg gagatagtgc atcttctgca gaattatatg tactattatc atctcttagt
    17941 ggagtatcaa ttaaacaaaa tattgctgtt acaggttcta taaatcaaaa gggtgatata
    18001 caagtagttg gtggtataag tgaaaagata gaaggatttt attctatatg taaaactaaa
    18061 gggttaaatg gtgagcaagg cgtcataatt cctagaaaaa atcaaagaaa tctcgtttta
    18121 tcagatgaag ttaatgatgc agtgcgagat ggaaaattta agatatatac tgttgaaaga
    18181 gtagaagagg ctatagagat attgacagat gttaaatttg aagaaataaa gaaattggta
    18241 aaagaaaaat taatatcttt cagcaagata caaactgtgt caaaagaata agtttattat
    18301 agaatttaat gggtatttat gtataataaa aagcaagaac tttaataggt ttttgctttt
    18361 tttgtaaata tatataaatt aaattcttat tttgtatgtt ttatatattg atgtttattt
    18421 ttttgttgat atgattaaac tatttataaa tagcgtttta taaattggta ttttgaaggg
    18481 ggataattat gaaaaatgta aatctaaaag agataaatga aaaagatgta attttggaag
    18541 aagagataaa taaaaaagga atggataaga aaaatataat tgagaaggaa gagataaata
    18601 aaaaaggaat agataagaaa gatataattg agaaggaaga gataaataaa aaaggaatag
    18661 ataagaaaga tataattgag aagaaagaga taaataaaaa aggaatagat aagaaagata
    18721 taattgagaa gaaagagata aataaaaaag aaatggataa gaaagatata attgagaagg
    18781 aagagataaa taaaaaagga atgaataaga aagatataat tgagaaggaa gagataaatg
    18841 aaaaagaaat agataaaatg attggtaaaa aagatgagat aattgatgag aaagatatag
    18901 agaattttat ggatattgaa gaagataaaa aaagtatcaa agtaagtaaa aaaagtacta
    18961 aaaaaggtga aacggagaaa aaaacaaaga agagtagttc taataaggct acacaaaaaa
    19021 caagtaaaga aactaaaagt aagtttaaaa ttaaaaatga gtcagcaaat gaaaaagacg
    19081 taaataagat tttagacgag ttagaaaaac tgtacccaga tgctaaatgt gagttaaatt
    19141 atgggacagc ctttgaactt ttaatagcaa ctattttatc agcacaatgt actgatgtaa
    19201 gagtgaataa agttacttca gagttgttta aaaagtacaa cacagctaga gactttgcta
    19261 atttgagcat tgaagaaatt agtaaagaga taaagagttg tggtctttac aaaagcaagt
    19321 ctcaaaaaat aaaggataca tctgaacagc tatgtgaatt atatgatggt gaagtacctg
    19381 atagcttaga aaagcttata aaattacctg gtgttggaag aaaaacggca ggggttgtac
    19441 ttagtaacgc ttttaatcat ccagcaatag ctgtagatac tcatgtattt agagttagca
    19501 atagaatagg aatagtggac gagccaaatc ctcaaaaaac agaatttgca cttatggaag
    19561 ctataccaaa agaaagatgg tcacactcac atcatgtatt aatttttcat ggaagaagaa
    19621 tgtgtaaagc tagaaatcca gaatgtgcaa gttgcccaat aaaagaagat tgtaattatt
    19681 ataaagaact taatgaaacg aaataacttt tagaaagtaa gttgtacaag agggattttc
    19741 tgatataata atacttgtga aaatgttaga taaaaaacta ttatatacat gaaataaaaa
    19801 ttattattaa cattatttta tataatagta taatattatt gttattctgt ttataaaata
    19861 aaatttgata aataaaagcg atacttaggg aggattaaaa tgtcattaaa tatagtatta
    19921 gtagaaccag aaatacctca aaatacagga aatataataa gaacctgtgc agcaacaggt
    19981 tcaactttac atttagtaaa accactgggt ttttcattgg atgataaaca cctaaaaaga
    20041 tgtgggcttg actattggga tatagctgat atacaatatt acgatagctt tgaagaatta
    20101 caagaaaagt atccaaatag taaatacttt ttttctacaa caaaggctaa acagtcacat
    20161 tcagatgtga aatatgaaga aaattgtttt atagtatttg ggaaagaaac aaagggatta
    20221 ccagaacctt tgttgaaagc aaatatggat acttgtatta gagtgcctat gttggatgtt
    20281 gaaaaagcta ggtcattaaa tttatcaaat tcagttgcta ttgtagtgta cgaggcttta
    20341 agacaaatag gatacccaaa tatgaggtag tttcaacaag cctaaatgag aaaggagcat
    20401 acttaatgaa aaatctaaag ataatctgtg acagtttatc ggacgtgcca aaagatttgt
    20461 tagataaata tgatatagat atagtttcat taacagttat actggaggaa agagaataca
    20521 aggatagaat tgatatatct ggagatgaat tttataaaaa gcttagagaa gaaaatgtat
    20581 atccaaaaac ttctcaagct acttatgccc aatttaagga aatttttgag aaatatacaa
    20641 atgaggatag agagatactt tatatagcag ggtctgccac agctacaggt acgtatcaaa
    20701 gtgctgtaat ggcaaagaat gatgtagatg gtaaaatata tacatatgac tctaacttgc
    20761 tttgtatggg aactggttta cttgtaataa aagcaggaga acttgcaagt gaaggaaagt
    20821 ctgttgctga aatattgcct gttttggatg aattaaaaga gaaggctttt gttatatttt
    20881 cagtagatac attggaatat ttacaaaagg gaggtagaat atcatctact aaggcagcaa
    20941 taggaagtat attaaatata aaaccgattc ttgaaataag agatggatta gtttcgcaaa
    21001 tatctcaagt aagaggaaag aagaatgtaa tttctaagat gatggaattg ataaaatcaa
    21061 attgtggtga tgatttatca gaccaaattg tatatatagg atatagtgat gattttaagg
    21121 aaaaagaaca attatctaag gttatgcaag atgaattaag acctaaagaa attagatttt
    21181 tccaaattgg tgtgtgtata ggtgcacatg ctggtcctgg tgttactggt atcatatgct
    21241 taaaaaataa ataatatatt tttggatgac attatttatt tttttattaa attaggatag
    21301 aatattaatt attgagatgt taaattggat ttattttgga tgaaattaag tgttgatata
    21361 ttagatttag tttgtaggga agtgtgtaaa tagataagaa tctattgcac acttttttat
    21421 agtaagattt attaaaaaaa tttcaaaatg tacttgtaaa ttgtatataa aaaaattata
    21481 aataggatgt aaaagtaaat aggatgtaaa agtaaatagg atataaaaat atcgtaaatg
    21541 tatgaggagt gaaatttaat gcgtttcaaa agaatattga tattgatgtt aactgtagca
    21601 atgatagctg gaatgttggt tgggtgtgct aataacccaa gcaataatcc tagctctagc
    21661 aaagataata aaagtgatat aagcgataaa agtaattcaa aaaaatctgt atcaatgata
    21721 ctagatattg aaggaacaaa taatgaagct atgaataatt cagctttatt agcattaaac
    21781 aatgctcaaa agaaacttaa tatagataca aacaaagtgg aatcagatga ttcctcaact
    21841 ttttctaata gtatagatat tttatgtaat gacaattacg atttaattat agctgtagga
    21901 gctagatttg caaaaccatt ggagatggta gccaagaaat atccaaagca acaatttgca
    21961 attatagact atgagtatga caagcaacct tctaatataa cttcaatttc ttatgaagat
    22021 aataaatctg gatatctagc tggattaatt gctggaaaaa tgactgaaag cgataaggtt
    22081 ggttttatag gaggaacaaa gagttctagt agagataaat ttgaatcagg ttttagagag
    22141 ggtgttaaat tttctaatag tagtataaag gatatttcag ttgagtatgc agatgttttt
    22201 aaagatagca aatcagtaga atctatagct aaaaaaatga tggataatgg agtggatatt
    22261 attttttcta ctactgaaga tgattcaaaa gctgtaatag atgcagtaag ggcaaaaaat
    22321 aaaaaagtta tagcaacgaa taaagaccaa catgagttag ctccagaaaa tgttataagt
    22381 tctattgtaa aagattttga gaatcctact tataatctga tacaaagttt tgtaaaagga
    22441 aattataaag gcggaaaagt tatagaaaac acagttaagt ctggaagtac tgggcttgcc
    22501 caaaatagtt ctcaaaatat tcctccagat gtgttagaat atgttaataa gaacaataaa
    22561 taatctacta taatatccaa gaatacttat gactttagtt atgaggtgaa ttggatgtta
    22621 taatagacat atatagaaag acatatgcag aggtaggtca gacctatcta aatcagggag
    22681 tgaattttgt gaaaataaaa aggatattga tggcttgctt tgtagtagtt tttacaattt
    22741 ttatattagc tatatgtaat tttgattttg ggtttgataa agtaaataag aataattcta
    22801 atatagatag tgctaaatcg aaacagagtg agtctaaaag tttgccaact tcagcagaaa
    22861 aggataaatc aaatagtaaa agtaagaata aagaagatga cacagaagat gattcatatg
    22921 taagaaaaac tattggagta ttgttagata ccgaaaactt aaataacgaa tcatataata
    22981 acaatgtact atcagcctta aaaagggctg acgaagattt tgatgttagg ataaaacttg
    23041 aaaaaccaaa gacttctaaa agttttaaag agagtataga aaaactgcat aagtatgaac
    23101 cagatttgat aatagcagtt ggagccagat ttgcagagcc aattaaagaa gctgctactg
    23161 aatatcccaa acagcaattt gcaatagttg actatactta taaagttcag ccagctaatg
    23221 taatttctgt atcatttcaa gataatgtta ctggatattt agcaggactt attgcaggta
    23281 aaatgactga tactgggaag gttggtttta taggaggagt tgagggttct agtagagata
    23341 agtatgaatc tggatttaga aaggggctta aaacttcaaa taaagatgct gagttactag
    23401 ttaagtattc aaatatattt gaaaaccctg aatcagctag aactattgca aaggaaatga
    23461 gagattctga tagagatgtt gtattttcag ctacagagga tgatagtaaa agagctattc
    23521 aggtagcaag agaaaatgga ggtaaagtta tagctattaa taaagaccaa tacgatatgg
    23581 ctccagataa tgtaataagt tcaattataa aagatgtaga acaacctgta tatgaactga
    23641 taaagaacgt tgcaaaagat ggttttaagg gaagtaaagt aatggaattt aaaataaaag
    23701 atggagaact tggcttgact tcaaagagca gtagaaatat accaccagat gttttggaat
    23761 acataaaaaa agagtataac aaagtgaaag atacatatta attattgatg tagaagatta
    23821 taaaaatata ctgcttaagt ttagattggt gataaaagga tatatttatt aaatttgtgc
    23881 taactgtaca aaatatattg attaagttta gattagtgat aaaaagatat atttattaaa
    23941 tttgtgttaa cagtatggaa tatactgctt aagtttagat tggtgataaa aggatatatt
    24001 tattaaattt agtctaactg tacaaaatat attgattgag tttagattag caataaaaag
    24061 atatatttat taaatttgtg ctaactgtac ggaatatact gcttaagttt agattagtga
    24121 taaaaagata tatttattaa atttgaacca actgtacaaa gctatactaa ttaagcttag
    24181 attaactata taaaattata acaataagtg tgtgggcaga aaaattctgc tacacacttt
    24241 atttatattc acataaaaaa tttatcaaaa aaaagaaaaa ggagtaaaaa atggaattta
    24301 caagagaacc atttatacta gatgacgatt tgtgtctagc tttggtggac aaaagaatta
    24361 gttatctaat ggaaaatgaa ttaaaattaa gaaatataga gataataaaa acaatagaat
    24421 gtaaagaatt atatgaaagc ataaaatatc acccggatat atgtatttgt aatttaggaa
    24481 aaggagatat aattgttgcg ccgaatgtat ataatcaata caaagagtta ttaagtagat
    24541 ataaatttaa tataattaag ggagaagcta atttattaaa tagataccct tatgatatac
    24601 cttacaatat tgcgatagtt ggtgactatg ctatacacaa ttttaagtat actgacaaag
    24661 caatattaga ttatattgaa aataataaac ttaaaaagat aaatatagag cagggctatt
    24721 ctaaatgctc aatctgtata gtagatagaa cgtctataat aacatcagat aaaggtattt
    24781 ggaaaagtat gaagaataca gaaatagagt gtttattgat agaaaaaggt cacataaatt
    24841 tatttgagat gaattatgga tttataggtg gatgtacagg gttgatttct aaggataaac
    24901 tagctttttg tggagatgtt aaaaaacatc ctgactatga acgtataaaa agttttgtag
    24961 aaagtaaaaa caaggagata gttacactta gctgtgaaaa tttactagat ataggttcta
    25021 tagttcctct catgacaaga aaggagcgat aactttggtt gagaaggata atatttcttc
    25081 catgaaattt ttgaggaata ataatgtaaa cgatagatta atatatacga atgacattgt
    25141 agaagtagtg atagattctg caaagcaaga agtgttaaat aaaaaaatac ttaatggtac
    25201 taaagctgaa caaagagctt taaatattga tgagatatca acggaaataa ctagtatgat
    25261 aataagtata atgaaagaga aacttttgaa agaatacatt ctaaaatgct atgggcgtac
    25321 atatcctgaa gataaaaata atatatacat atattcactg aacatatttg ctaaaaaaga
    25381 gatttttatg agagaaacag tatttactag ggtgagaaat tatatagaag aaaatgattt
    25441 tataaatgta gaaggtttta ttagatttag aatgagagaa tttatgaaat atatttctgc
    25501 tataggtgat attgcagttg aggagtattt aataaagaaa gaccaagatg aatttataag
    25561 agttttaaaa tactttatag atactcaaga agaaaagatt gatttgctta aagttcatat
    25621 aatggaagat aatacatttg ttttatacga taaaaatgga aataagatag acagtataga
    25681 tgatgaagaa attataaata tggtaataag agaaaattta aattatgagg actttttaat
    25741 aagtacctta ttaactctat gtcccaaaaa aatagaaata ttggatttgt taaataataa
    25801 ttgctcaaag gaaattatag atactgtaga agcaattttt gaaaataaag ttagtataat
    25861 tatggaaaac tagaatctaa gcatataaaa gctatccata taaaattaaa aaaattcttt
    25921 ttaaatcgac acaaaagact tataattgta tataaagcta tagtataact agtgttatta
    25981 agtataaaga atatctactt gattaataca atatagctgg caaatataca attgataagg
    26041 agtaatttta taatggataa tttaattagg atactgatat tagctgttat tggtggattt
    26101 ataggttatg ttacgaatgt agttgctata agactgatat ttagaccaat agagcctatt
    26161 aaaataccta ttttaaatat agaaatagta ggtcttatac caaagagaag agcagaaata
    26221 gcagcaaatg taggagaaat aattcaagaa gaatttcttt ctatggacga aatacttgca
    26281 aatatcatta ctgacgaaga taaggaagaa gttgtaagat acataaaagc aagggtaaaa
    26341 attataatac atgagaaagt ttcatttatc ccgagtggta taaaaaacat gatacaagat
    26401 tatctaggag agataattga gtctgaagtt aagcaaagta tagatgaatt gagtaaaaat
    26461 ataataaata aagcaaatga acgtatagat atacaaaaaa tggtcgaaga taagattaat
    26521 gaactagatt tatatgaatt ggaagagata ataataagaa tagctaagaa agaattaaaa
    26581 catatagagt ttttggggct tgtcttaggg tttttaatag gtatagctca agggttaatt
    26641 actatgttta tttaaatata tgagaagtag catttattta agtactaatt atatttacct
    26701 atttgagtga attacttatt aatagcattt agatattaat tgtatttatt tatttgattg
    26761 tgttacttat taatagtatt taagtattta ctgtactact tattaactat attaagtatt
    26821 gattgtattt aagtatacaa aacctaaaaa gagtacaaaa attagaagtt agttttaata
    26881 aaaattagtt ttagtattta tctagtcata tctatcatgc atgtggatac gttttaaaaa
    26941 ttttaaattt atgaaaatat tataatatca ctataaatca agagattaat ttaatataaa
    27001 aatataagaa tattggttta cagctgattg agatgaaaaa taatgggtaa ttaatatatt
    27061 acattaatgt tgattgatgt ataactatct gttataatga aaatatatta aaaatcatag
    27121 tgttgaagag gaagagtaga gtagaaacca ttctcagaga atttgggtct atggctgaga
    27181 gcccatgtaa taggtctatt tgaaaactac cttggagctg agcttattag cagtatttgt
    27241 tttgtactat acgtgaaagt gctcgggtga cgaccttata gtcatcaaga gggttgtata
    27301 tacttgtata cgccaacttg ggtggaaccg cgaattaata tcgtcccaaa catatttggg
    27361 gcgatttttt attacaaaaa atctaaaaat tatttcaata aggaggaaaa aacaatgatt
    27421 aaagttgctt taaaagatgg ctcaataaaa gaatttgaaa atgctatttc tgttatggat
    27481 gtagctaaat ccattagtga aggattagct agaaatgtgg tagcagcgtc tgtgaacgga
    27541 gaggttgtag gtctggacca cattatagat actgattgtg acttgaattt atttaagttt
    27601 gaagataaag aaggtaagga agtttttaga catacatcag cacatatatt agctcaagct
    27661 ataaaaagac tttacccaga agcaaagctt gctataggac caagtataga aaatggattc
    27721 tactatgata tagatttaga ccatagatta gttccagagg acctagagaa aatagaagct
    27781 gaaatgaaaa aaatagctaa agaagattta aaaatagaaa gatttgaact tcctagaaat
    27841 gaagctttag agcttatgaa agaacaagga gaagactata aggtagaact tatatctgat
    27901 ttacctgaaa gtgaaataat atctttctac aagcaaggtg attttactga tttatgtaga
    27961 ggaccacatt taccatctac taaaaaggta aaagctgtta aattacaaag tgtagcaggt
    28021 gcatattggc gtggtgacga aaataataag atgcttcaaa gaatatatgg aacttctttt
    28081 gagaaaaaca aagacttaga agagtactta catttactag aagaagctaa aaagagagac
    28141 cacagaaaat taggtaaaga attaggatta tttatgatac ctgaagaagg tccaggattc
    28201 cctatgtttt taccaaaagg tatggaactt aaaaatgaat tattaaaatt ctggagagaa
    28261 atacacagaa aagctggata tattgagata gaatctccaa tcatattaaa tagaaaatta
    28321 tgggagactt caggacactg gtatcattac aaagaaaata tgtatacagt taaaatagat
    28381 gatgaggatt atgctataaa acctatgaac tgtcctggtg gattaattta ttataactct
    28441 caactacatt catatagaga tttcccaatg agagttgcag agcttggtag agttcataga
    28501 catgagttat ctggtgcatt acaaggatta atgagagtta gagcatttac tcaagacgat
    28561 tcacatatat tcatgttacc agagcaaatt aaggacgaaa taaaaggagt agctaactta
    28621 atagatggta tatacaaaac ttttggattt gaatataatt tagaattatc tacaagacca
    28681 gagaactcta tgggaagcga cgaagagtgg gaagcagcag aaaatggatt aagagaagct
    28741 ttagaagagt taggtctacc atatactatt aatgagggtg atggagcatt ctatggtcct
    28801 aagatagact tccatctaaa agattgttta ggaagaactt ggcaatgtgg tactatccaa
    28861 ttagatatgc agcttccaag acaatttgat aatacttata ttggtcaaga tggtgaaaag
    28921 catagaccag ttatgattca tagggttgct tttggtagta tagaaagatt tataggtata
    28981 ttaatagagc attatgctgg taaattccca gtttggttat ctccaactca agttaagata
    29041 cttcctatat ctgataaatt tatggattat gcaaatgaag ttaagaaaga gttatttgat
    29101 aagggtataa gagttgaatt agatgataga gctgaaaaaa ttggatttaa gataagagaa
    29161 gctcagcttg aaaaagtgcc atacatgcta atagtaggtg aaaaagaagt agctgataat
    29221 aatgtttctg ttagatcaag agataaaggt gaaataggta gtattaaatt agatgaattt
    29281 atagctagta tttcaaaaga aattgaatca agagaaagta ttattcaaga ctaatatata
    29341 ttatattagt agctaagata ttgttatata agataagttt aatcaatatg ttgatatgtt
    29401 gatgcatagt gattaaattt atgaaaagat gagtataatt agaggtacag ttaatcatat
    29461 ttttatgatt aatgtacctc tagttttttg tatggtagat ttattaataa atatatcata
    29521 gaagtgtttt gaacatggaa atatttatgt gcattcttta aataaacgtt tattttttga
    29581 gtgataatta agtgcttgtt ggtaaggtta tggctgtaaa aatcataact ttattttcat
    29641 atttgttcca ttttcataaa aattattatc cctttaagaa aatttcttaa tcctcttgaa
    29701 atatttcctt tttagtctaa agttaaattt ctttcttcaa aatgaatatt taaatgaact
    29761 aaaatttaag tacattttct aatcttatga gtctattttt catagataat tgccctataa
    29821 tagaagaatc tatgttggtg ctatgtggtg acaataacag cattgtcagt gattaattaa
    29881 tgctttataa atgattaatt agtgttttat gttatttttg gacttaccct ctatacatag
    29941 attaatttat ttaactttag atagagagag aatgtcaagt acatacacag acattagagt
    30001 ttaagaaagt gtttatggag caagaacttt taaaagaggt agtgacttag tttaatttca
    30061 agaaatgata aatacaaaag aggaaaaaga gaggtgttga aatgatagaa aaagtagaag
    30121 aaaaatctaa gattattaaa acatcaaaag aatataagcg aactataatt gaagaaaaag
    30181 ataaagaatg gaccaaaaaa ttaaatgata tacttagtag aagtagtttt tctaatgatg
    30241 aattagataa aatcaaggcg ttaattccaa aagagatttt gactaatgaa aatgttggag
    30301 aagttgtgac tgaagatgga tatgctaagc ccgagtatga tgaggtattt aaatcattgt
    30361 tcactttaaa taatgaatat gatttacttg taaattttat aaatgatatt ctaaaagatg
    30421 ctccatatgc taacaggaat atcaaaccgt tcactcaaat aaagagaata attagagttg
    30481 aaactgatcc aacaattaat tacattggag agaaaagacc taaacttgat attttggctg
    30541 aagatgaaga aaataatcat ataaatattg agatgcaaag agcttttgag aacgattatt
    30601 tggaaagagc tgaatattat cttagtcgtg tacatggtag aaagttagaa gaaggtaaag
    30661 agtataaaga aattggaaaa actattggaa tacatatact aaatcatgta aaatataatc
    30721 acatagaaga ttatgtaaat tgcctgagat taacaatgga cgggcatcca gatatttatt
    30781 ctagtaaaac tgcattatat tttatagagc ttccaaaaat tcataaatct gactatatag
    30841 taaatagaat tatgctatgg ggaaaactta ttagtaatcc ttctaactta gatgtaagag
    30901 ttatagctaa gaatgattct attattaaaa aagcacttga tagattaaaa gaattaggta
    30961 gcaatgaaga atatttatta aatctgaaaa gaggtgctta tatcatgaat aaaagtagaa
    31021 attttaaaga agagattgaa agagagacag caattagagt tgcaaaagaa aaagattata
    31081 aaataattat aatgttgaaa aaaaatggtt ttaaattaga tgacatatta aataaattta
    31141 atgattttga cttaagtgaa gatgagataa aagaagtata tgaaaataat tgatgataaa
    31201 taaaaagtga acttatataa gtttgctgaa tcggagtctg gaaatattta tgtgtatttt
    31261 ttaatgaatg aaaatttatt ttaaaaacaa taatttagaa gtacagttaa ttatattttt
    31321 atgatactgt acttctagtt ttttctattg tagatttatt atttactaat aaatctacaa
    31381 tagaaatgct tttgttgtaa gaatcatttt atctaatgat tgttttttat aatagttaaa
    31441 tttccatttg tagtgtttaa atataaatct ataacagcat ttctataaat gttataaatc
    31501 actataatga ttcaactcaa agttactgct taaagaagca gagattttat caaggtatat
    31561 catggaaaaa aatattttat tgtaatattt ataagatttt attatggaa
//
LOCUS       DAMJWS010000043        31019 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMJWS010000043 DAMJWS010000000
VERSION     DAMJWS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.043"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(111..647)
                     /locus_tag="PC399_002089"
     CDS             complement(111..647)
                     /locus_tag="PC399_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2592947.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(665..1978)
                     /locus_tag="PC399_002090"
     CDS             complement(665..1978)
                     /locus_tag="PC399_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2592948.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2333..2773
                     /locus_tag="PC399_002091"
     CDS             2333..2773
                     /locus_tag="PC399_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2592949.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2958..3146)
                     /locus_tag="PC399_002092"
     CDS             complement(2958..3146)
                     /locus_tag="PC399_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592950.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            4193..4588
                     /locus_tag="PC399_002093"
     CDS             4193..4588
                     /locus_tag="PC399_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592951.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4631..6730
                     /locus_tag="PC399_002094"
     CDS             4631..6730
                     /locus_tag="PC399_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF2592952.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYNGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6955..12165)
                     /locus_tag="PC399_002095"
     CDS             complement(6955..12165)
                     /locus_tag="PC399_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2592953.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKEIM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNALYEEHKDWIISKREDSGSA
                     GAEGTAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILKSLGYIDFKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12180..14675)
                     /locus_tag="PC399_002096"
     CDS             complement(12180..14675)
                     /locus_tag="PC399_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2592954.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15358..15645)
                     /locus_tag="PC399_002097"
     CDS             complement(15358..15645)
                     /locus_tag="PC399_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592955.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15665..15940)
                     /locus_tag="PC399_002098"
     CDS             complement(15665..15940)
                     /locus_tag="PC399_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592956.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16577..16756)
                     /locus_tag="PC399_002099"
     CDS             complement(16577..16756)
                     /locus_tag="PC399_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592957.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(17041..18516)
                     /locus_tag="PC399_002100"
     CDS             complement(17041..18516)
                     /locus_tag="PC399_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2592958.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
     gene            19585..20763
                     /locus_tag="PC399_002101"
     CDS             19585..20763
                     /locus_tag="PC399_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2592959.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFRNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            21113..21994
                     /locus_tag="PC399_002102"
     CDS             21113..21994
                     /locus_tag="PC399_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2592960.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKISNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            22185..22352
                     /locus_tag="PC399_002103"
                     /pseudo
     CDS             22185..22352
                     /locus_tag="PC399_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(22612..25179)
                     /locus_tag="PC399_002104"
     CDS             complement(22612..25179)
                     /locus_tag="PC399_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2592961.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSNP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRAIKSGQMITVDGLAGTVTFDE"
     gene            complement(25182..25787)
                     /locus_tag="PC399_002105"
     CDS             complement(25182..25787)
                     /locus_tag="PC399_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2592962.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(26161..27621)
                     /locus_tag="PC399_002106"
     CDS             complement(26161..27621)
                     /locus_tag="PC399_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2592963.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            27975..28553
                     /locus_tag="PC399_002107"
     CDS             27975..28553
                     /locus_tag="PC399_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2592964.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYSNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            28604..28837
                     /locus_tag="PC399_002108"
     CDS             28604..28837
                     /locus_tag="PC399_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592965.1"
                     /translation="MIELIQRLNDIIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKFLIIIAIISIIINILIILDR"
     gene            28846..30096
                     /locus_tag="PC399_002109"
     CDS             28846..30096
                     /locus_tag="PC399_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2592966.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            30257..30826
                     /locus_tag="PC399_002110"
     CDS             30257..30826
                     /locus_tag="PC399_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2592967.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT        10471 a         5289 c         3752 g        11507 t
ORIGIN      
        1 attttcctac tcttttttct gtacttacat ataatattaa aactctttat tttttaatat
       61 atattttaaa ttttcttttt taataaatct ttttctttta ataaatctat ttaattttct
      121 tcttctatat cagagccaaa gggaatccat gcattaccaa actctttatc tttaaataaa
      181 taagctaatc cagttatttg cttcaatcta agaatttcat caacttccat acccaggtgc
      241 tttgatatcc attcatcaga ttttccaatt tcacaaagtt cagttataat tttactcatt
      301 aaatccacat tatgagaacc tcttgcacga ttgtgtcgaa tagtacttgc cattctttca
      361 tcaagagatt tatcaattac tgaaacagga agaagtccat tttctctatc atatatatcc
      421 tcattttcaa gcattactct atatctatga aaaccatcta caataatata cttatccttt
      481 tcctcattat aataacagac aattggcatt gtatacccat cttcttttat acttttatac
      541 aacaactgca tttctggtgg ggctactgca tttggattat agtcgttaga ttctatcttt
      601 tcgattggca ctgcgatgat tccatataca ggacttttat attccatagc atcttaactc
      661 ctatctaatg aattttttca tatttcttct taatatattc cgttctaatt ctctgttctt
      721 ttgtaggacc aaatcccata aatctacaca tatggtcatt ttttaaaata caataacaca
      781 ttcttttcca acttggtata tctttggtaa atttaatatc atccgtatca tcaggtatat
      841 catccaaaaa tatgactcta ctctttttat cttttgtatg atttgaaact ccatttaatt
      901 ctattttata accttttctc aacaattcac gtataactat ttctgacaaa ccaccacctg
      961 tattctgcca aaactcaata gatgttgaaa attttttaat atagctattc cttatatttt
     1021 caggtaaggt atcaagcaaa aaatatgtga aacttttcca tgtgtgtcca tatggcaatt
     1081 taacactacg atatcccata gcttttgttt tgccataaat agaagtaaaa ttaactcctt
     1141 gtactctagc aaccaatttc acccatacat ctgggtccaa tattctgtat aaatttaaac
     1201 tctcttttgc tgaatcatta aaaggtgatg ccactctcat ttttcttgga gctactccag
     1261 ctttatagaa taagtcataa attttattat agtcatagtc aaatttatag tgtgctaacc
     1321 atatatcttc tatttcccaa tcatatatag gagatgccgt ccagacattt ttaaattcct
     1381 tagttatcca gcactttcct ttatatccat gctgtttatt cacaatagaa caataacgtt
     1441 gtagagattc tgctgctcgt gttcctaata agcaaacaac ttttgcacct tcatgtatat
     1501 ctctaatcca tctaccaaat tgttttgcca aactttcttg tctcatttta tatttataat
     1561 aagtaaaagg attatttttt tgatttatta tgtattcttt gactggcatt tcccttaccc
     1621 ataactcttc tttctcatca tcccatggat accaatacat ttcataattt cctacagcag
     1681 ttcttgtagc cattggtaaa cacacccaat atggttcaat atcttcaata ttatcttcaa
     1741 atactttttc aacatattca gtagtcaaac tatattgagc ctcaaaatct tgatgaaata
     1801 ctcctatttt ggaacttata ttattctttc tcttataatc caaaactaaa tttagcagta
     1861 aaccactatc ttttcctccg ctaaaagata catagatata atcaaattca ttaaaaagat
     1921 aagaaagtct ttcttgaaaa gcttcaaata catcggattc taaatatctc ttatccattt
     1981 gaattatccc ctttcaatta gaagtttata aaaccttaca atgcaatatt attatgcttt
     2041 tttagcagta atcagcataa attatacatt atttaaatac acaaatccaa ctaactacat
     2101 ataattataa tgtaattata cttttgtatt ataaactata ttttatcatt attttacatt
     2161 tttttccaat atatataaat atcatatttt tctattagct aaaactaatt actttatatt
     2221 tctacgtatt agtataataa tacataaaaa cttgaataat gcataaaaac ttgacatact
     2281 gttgtcataa ttactttgct ataattaaaa gtaaaaatgg aggtaaattc taatgtatga
     2341 acgtatgctt gataaaagca ataaaccctg tttgtctcaa ataatagaat atataggtaa
     2401 agatggatat gcacgattgt gtaagttgga aacttacttg aaaactcaat ataatttatc
     2461 aagagaactt cgatttcctt ttggaaacaa ttatggttgg ggttacaaat atagccataa
     2521 atcttttcat ttattttatg ctttttttga aaaaggtgca tttactgttc tgattcagat
     2581 aggtgacaag caagttccag cagtagaaaa aacgttggta gctttgacac caaaagctaa
     2641 agagctctgg gtcactcgtt atccttgtgg caataatgga ggctggatta agtatcgtat
     2701 tttagaagat gcagacttac ttgatattat tcaatatata agagcaaaaa aatctccacc
     2761 taaacaaaat taacttttat tttttgtgat ttaaatatag tgtatataaa ttccattctt
     2821 tccatataaa aaaaataata taaaaaatat ctattagtat aacttattta gtatgtctta
     2881 ttctactttt tacagataat taaaaagttt tatcataaat aaatcataac tagacatcct
     2941 agtaaatcaa aattaattta ttctttttta ttagaatttt tgataacagt ccctatacat
     3001 atgccaatta tcattccaaa aattagacca taacacattg tgatacctac atttccttta
     3061 gaaataccta tagcacaacc tatagccatt ccaagtatca tgcctataaa catacatata
     3121 ataactaaat tgttgttttt attcatagtt ttctccattc aatatatttt aaattgccta
     3181 gctcgccttc ttagcttaaa acaaagaaat ataaattaac tttctttcat tctaatatct
     3241 gcttgtataa atgtcaaaca acacatttct tgtcataacc tcctcttttt gaagtaagta
     3301 caatctccca gacgcaataa tatcaatttc ctaaaaccat ttaagtaact tttatattta
     3361 cataatattt acaatcataa actatatttt tattcattta tacatcataa acactcccta
     3421 ctaatcatta ttatacttaa aataatgttc atttataata atatatctaa cacactttaa
     3481 ttgaaaaaaa taaaactttg cctttcattc ccatatataa ataagtatac tttttgatgc
     3541 tctattacaa atcaactaaa ttaattgata taaattatca ttttctgttg aaaataccat
     3601 tcgcacatgc tatacttaat tgcataaatg gtaataaatg tcatatgatt ttacaaatct
     3661 attaataaca attattactt ttactgtatt ttaaattttt tggactaaat atcaagaact
     3721 ttctaaaata caaagtacaa aaaatttata taagttataa atttatatga actagataag
     3781 ctcaaacaat atgttatgta taaactgtgt agtattcggt tataaattta tataaattat
     3841 ataagctaga taaagtatta cctcaactat ataagctaga tatataaatt gcatatataa
     3901 gctatataag aattatttca actatataaa ctacatatgt aaattatata cataagctag
     3961 atatataaac tagatatata aactagatat ataaactgca tatataagct atataagaat
     4021 tatttcaact atataaacta catatgtaaa attcatgtaa atttactaat ataaatcaaa
     4081 taaaattact aaactctcta aactaatttt gtaaaacata ctctattttt aacatttgtt
     4141 accttataca taagtacaaa caaaatctaa ctaaatgaaa ggataatgta aaatgaatat
     4201 tttaaaaatg aaaaaaacag ggatattttt aggaggagta ctttttggta ctgtaggtgt
     4261 taagatactt tcaagtgatt gtgttaaaaa aacttgcact cgtgcaacag caggagcatt
     4321 gaaagttaaa gattctgttc ttgaaacaac tacgaaaatt caagaaaatg tagaagatat
     4381 tttagctgaa gcaaaagaaa tcaacgagca agaagactta tctacatcta aggtaaacgt
     4441 tgtacaagat actcttaaat gcgaacaaga agttcttaaa actgaaacta taaaaactgt
     4501 agaagatact aattctaata gcattaaatg tgaagaaatc aaagataacg taattgaaga
     4561 aaaaaatagt acttgtacaa cagaatagat ttaatgatta ttatgattaa taatatcagg
     4621 aggaagtaaa ttgaattttt atataaaaca cgaaataaga ggtcgtatac gctttgactt
     4681 acaacttggc aaattaaaca acaagcagtc cgatactctc ttatattatt taacttctat
     4741 aaatggtatt accaaagcaa aagtttacga tagaactggt gatgcagtca tttattacaa
     4801 tggtgaaaga gaatacataa taagagaaat cactaaattc agttttaaca atagcaaaat
     4861 agaaaaccta gttcctgaac ataccagcag ggaattaaac aattattata gagacaaatt
     4921 agcaaataaa ctgctagtca aaagtatctc caaaatattt tttccaaagt ccttaagatt
     4981 ttttttaatt ggacttcatt ctataaaata ttttaaaaaa ggtctctctt ccctgctaaa
     5041 taaaaaaata gaagtttctg ttttagatgc cactgctata ggtatttcta tttttagaaa
     5101 agatatcaat acggctggtt cagttatgtt tcttcttggt atcggtgaac tattagaaga
     5161 atggactaga aaaaaatcta tagacgactt ggctcaaagt atgtcactaa acattgaaaa
     5221 agtatggtta agaaaaggtg acactgaaat cctaattcca atttcagaga taaaggaagg
     5281 agatttagta agtgtaacta tgggaaatat gattccattg gatggagtta tagtatcagg
     5341 agaagcaatg gtcaatcagg catcacttac aggtgaatcc cttgcagtta acaaaaaaga
     5401 aggatcttac atatatgctg gaactgtaat agagcaaggc aatattgtta tgtgcgtcaa
     5461 ggaaaaagct ggtactacta gattccagaa aatagtaact atgattgagg aatctgaaaa
     5521 actaaaatct tctgtagaga gtaaatttga acatctagca gatacattag ttccatacag
     5581 ctttttagga agtatattaa cctatgcaat tactagaaac cctataaaat cactctctat
     5641 attaatggta gatttctctt gtgcattaaa actctctatc cctatttcag tactatcagc
     5701 catgagagaa tgtaacaata acaacattac tgtaaaaggt ggaaaatttc ttgaaggtgt
     5761 tgcatgtgca gatactattg tatttgataa gacaggcaca ttaacaaaag ctcaaccaac
     5821 tgtttcagat ataattacat tccaaaatta taataaggag gatatgttaa gacttgcagc
     5881 ttgcttagaa gaacacttcc cacactccat agcaaatgca gtagtttatg aagctgaaaa
     5941 acaagggctt agtcacaaag aaatgcatac agaagtagaa tatattattg ctcatggcat
     6001 ttcaacaaaa gttgatgata aaaaggttgt aataggtagc aaacattttg tatttgaaga
     6061 tgagaagtgt actatcccag acggtgagga ggataagtac aataatctat cagatgagta
     6121 ttcacaccta tttatggcaa tatcaggtaa attatcagca gttatttgca taaatgaccc
     6181 tttacgaaaa gaagcaaaat atgtaatttc aaatttaaga gaatgcggaa taaaaaagat
     6241 tgtgatgatg actggagata gcgaaaaaac agctaaatct atagcaagta aagttggtgt
     6301 tgatgaatat tattcagaag tcttaccaga agacaaagcc aattttgtta aaagtgaaaa
     6361 actcaaaggt cgcaaagtaa ttatgattgg tgatggaatc aatgattctc ctgctatttc
     6421 agaatcagat gtcggaattg caatgagtga aggtgctgaa atagcaagag agatttcaga
     6481 tattaccata tcagcagata atttgaataa tttaatcgtt ttaaagcaga ttagtaataa
     6541 gcttatgaaa agagttgatt taagttctaa gtttattatc ggctttaatt taggtttgat
     6601 tttacttggt gtaggtggat ttgttagacc aagtacatct gcctttttac acaacgcatc
     6661 tactgtcgga ataagtttaa acagtatgac aaatttattg gaaaacacca atacttataa
     6721 ctaccattaa tctccattac ataaaacata tgatataaaa aatttataat tcatcttttt
     6781 aaaaaaatat gtcttaaaat tagctttttt ctaatcaaaa ttatataaag catactttac
     6841 aaattttaaa ctcaacttat ttctattttt tacaaaaaag gagatgattc aaattttcat
     6901 tttgaaacat ccccttttgt aaatttcttt aaaatttata tattaattat attttcaacc
     6961 aataattgta ttttcaacca atctttcttc atagcatttt aaatcatttt gctttagata
     7021 attaaattca gactctacat aaataccaca gcctaccaat tcaattgtca aattaggtat
     7081 attttccttt agtatactag tctgctcata aataattctt tccaataaat caacttgtaa
     7141 tatagaaaaa tgcttcataa tctccccatc actatctata tctttcttga tttcatacca
     7201 actctttgac aaaattccct taaaatcaat ataccctaga ctcttcaata tatctatact
     7261 atcgctccat gcatacttac taggtttatc aaagtatgac tctagtttct tcttctgctc
     7321 atttgtaagt gaatcataag aaagcttctt attaaaatgt gccttcaaag aaatcttagc
     7381 catagaatat ggacttctac cagagtttaa tcctgcattt ttgaactttt caattccttt
     7441 atcttctact gaagaccata ttgagtttat tgaatctgaa tcattgtatt taacatcaag
     7501 accatgtgta atatttatat agttttgtgg aaatacatat tgataataga catctttttt
     7561 atcatcatat agagagaagt atccactatc atataaatga atatgcacct ttacttttaa
     7621 actatcctct aaaatatttc gtctaaggtt cgggatattt gcatacatgg ttacatattt
     7681 tatggcatca aagtttttat ttaatggaac ttcaaaacta atttcatctc ctgtcaaaag
     7741 ctttttcact tcttcttcat tacaatcata tagagaatat gtattttcaa atcccacacg
     7801 tgatttccca tctaaactgc ctgttaatgc taccttcaaa atatggttat ctgttttttg
     7861 ccattggtaa gcatcttggt caagaaactc tctaatctgt ctattttgct ctttcgcctg
     7921 atttataacc tcgttttcat cttctgtaat ccctacatgg catctattta agatatttac
     7981 tatattgctt ccaaatggga atgtccatag ttgaaaagct gctgcctcaa cctgacacaa
     8041 gtatctaacc tgatttaatt caatttgatg gataagtttt ttctgatatt cattcatctg
     8101 attaccacta tctgacattt gataaatcaa ttctcccatt gcaagattat cttttttctt
     8161 atcaatctta tcagtaatga taatttgagt ttccatcata tcctgtactt ttttaaatat
     8221 tatagaaatc tcctctgaat tttccatttt taagcttcca aactttgtgc ttaattcagt
     8281 taaaacttcc ttcatcttta atttacttat ctcattttca aaaatatctt gatttttaac
     8341 tgtactatcg aatttttcta caaatggtat aacagattct ttcaaagttt tatctttaaa
     8401 ttccaccatt tgagtattca attcatctaa tttcttaata ttattttctt tatctttaat
     8461 tgcctcttta actacctcta ttttactctc aaaactagat tttacattaa gacaattatt
     8521 aacaccacta atcgcagtat ttataagaga acaagcagaa cttccaatac tcaatgcatt
     8581 tttaatttta gatgtctttt gctgtctttc aatagcttgt ttacttttct ctagtgcagc
     8641 taacttatct ttttgctgtt tattattgta atttgcctcc aacataccta tttctttttt
     8701 ctggattttt gtgtaaattt ccttatactc gctttcactt aacccctctt ttgctctttg
     8761 aatatcgtca gctttgtctg cataattatc atcatctata gacttaacta taatctcctt
     8821 aatttggtca aatttagttc ttctttcttc atcaacacct gccaaattat aagtcatttt
     8881 atctaaagca ttttttctat cttcacttag ctgttctcca agtacaaatc tcataccatc
     8941 agaatccaat ttaaaatctg aaaaattaac catcctgtta gtccatttgt attcctcttc
     9001 tttagctgaa agattttcct tcaaagattt tgcactgtca aatgccccta gacaagcttg
     9061 accaacacca ctaaatgcag tcagtaaact caatgcgctt tgtattccag aaagaattaa
     9121 ctgtgttttc atttgttcct caagttttgc ctgtttcttt tttagttcct ctatgtcttc
     9181 ttgttcatct tctttcatct ttataatttc ttgaatcaac aggttcattt ttgcattaac
     9241 agaaagcatt ttattctcaa gttcattttt taaaacctct atttgttgat ttccagattc
     9301 aatttgagtt aggatacttt ccttatatgt attgaaatat ccttctttta actcatcttt
     9361 cttcccttgt tccaattctc ttaaggtctt atccagataa tctgtcaaag aacgaatatc
     9421 tgtaacacga tttcctccaa tttcactttc ttcaatcatc tgcacataaa atgctgtctt
     9481 ctgctcaagt gcaaataaaa tcatactcca ttcgctaaaa ttctgatttt ttcctacaaa
     9541 agattctatt ctatcagact gcatcaattt atttataagg tattcataga tttcctttgc
     9601 ttctttataa aattctttct ttgtattcat atcatccaga gaaagtactg ctttattttt
     9661 cttctcaaaa ctatagtact tctgaagtat ttctctattt aattgcatct ctattttctc
     9721 aactgcatga aaaatttcat aaatatgtga aagttctaaa atatcagctt ctaaaattcc
     9781 tactgcacac aacagtctaa gatttctata acctttaatt ccagaaaaac caaatctctc
     9841 gaaaaattca tagaagaatg tatctattga caaagaatac tcctgcttta acaattcctt
     9901 ttcttttagt gcatctccag agtgaccggc tgttccctct gctccggctg aacctgaatc
     9961 ttcacgtttg ctaattatcc agtccttatg ttcttcataa agagcattta atttctctcc
    10021 atcatctgct gcttgcttct gatttaaagc atgattaaac acatatctat caaaataata
    10081 gtagtttcta tcactattat ccattggagg agaggtacct atatatctta ttgtttcttc
    10141 atatatacct ctaattccac caagacctcc tgcaccacca gctcctccag agcctccttt
    10201 tcctgctaaa ccatgatttc catctttttt acttatagca ctttttaata aaggagcttt
    10261 tccaaggaga taaacctgtc cagacttgct tccaagacct gcatttccac cattacctcc
    10321 tgtgcctcca gttccacctt gttttccttt ttctgctttt accacatagt attttgtacc
    10381 tttaacatta gcaaattctt ttgactcttt tttagaacct attttagcat ttgccccagc
    10441 ttctccagtt atccctgtat ctcctttttt tccatcttgt ccatttccac cacgtccacc
    10501 atctacgtaa agtttaagtt ctcctctttt ttctatttct ttaaaagata atatgatatc
    10561 acctgaagta cctgcattat tgccatttct tccattcatt ccgtcgtaaa tagaactttc
    10621 tccaaccaat ttttctgcat ccttaccaga tacgttgatt gaataagtcc cttctggtat
    10681 atgtaatttt tctgtaagta gtacaatatt cttacctgag aagacttctt tctccatatt
    10741 acagtcaatt ttcaactcac aatctccata catatatata gatgacaaaa tattaagttg
    10801 ataaccagat attattttag tcaaatccga taaattcact ttaggtatag gaatatatat
    10861 agaaagtaca ttttctttct catatgattt tatatatgta caactttgaa tcttctctat
    10921 cctatactct gcatcttcaa gctttttggc ttcttcttcc ctatattctt cttctgctat
    10981 ccaatatttg atatctttta atgtatcttc taaagtattt tttagcaatg attttaacaa
    11041 ctcacttaga ccaacaatat gagtctcact acagtaacta tttaactcta aaagaagacg
    11101 tgtttcttct aatatattca ctttctgtac gccatcacca tatgaaagtt cttgtacaaa
    11161 ctccataatc tctttcatat tattagtcct actacataaa tctgtataca tcttatctaa
    11221 ttgaatcttt ttatctttta gactatttat agaagataga atggaaatag aatcttccat
    11281 acctttttta acactttcta tataaggaac taaaagtttt tctaggtctt tataggttac
    11341 atcaacagct ttttgtattt ctattttaca agaagcagta agcatcaacc ccaaatctgt
    11401 tttttcaaat gtttcaggta gatttttcat ttcatccaac aacttttctg gctgtttatc
    11461 tagtggatta cataatttaa tagatacacc atctgataat gctacattta ataagtacac
    11521 taattcctta caaccgtcgt aatctttctc ttgactatct aggtcttctt ttgctttttc
    11581 caatatattt cttatttttt cttctgttac attaccaatt agtgttttag gtattttatt
    11641 gataagaaag aagaaatttt ctttatactt tgcataatca ccaagcatat ctttcaaatt
    11701 tttgcctgtt tctatcaata atgtatgtct agagtccagt agggattgat agtctaaaat
    11761 aactaccatc ccttttacct ttgaagatgt ggaaatagtt tcccttacaa gcatagcacc
    11821 agcaatcata tactctgctc cacggttatc aaaaaaacct gggcaatcac ataatgaaaa
    11881 atctctattt gcaatatcta caacttctgg acataaagtc ttagaatata caccatttcc
    11941 aatttcgaca gctgaatctt ttgcagttat atatatttgt ccagtctctt catccgtttt
    12001 ttcttccatc acacagcctt ttaaataatt tatacaagta ctctttccaa cgcctgaagc
    12061 acctaagaat aacaaaattg acttttcttt taagtcatat tttctaagct cgtctcttcc
    12121 ttcttccaaa aggtttacga tttcactata aagtttattt gccatacatc catccctttc
    12181 tatatttcgg aacaatagcc ttgatatttt tctttaataa gattctcttc tacctttgaa
    12241 acagctgttg ataacttagt gtttggagca ttaaatcctc cattgtctcc tctatctcca
    12301 tattcagcgt attctcttgc tggtcctgtt ttaccttcat tccagcttcc accactaaat
    12361 tctatatgat atttaattat actatctaac ttataatccc aacttgtaaa atatcctgta
    12421 tagtatacag tcttatctat accacttgga gaaaatgaaa gtgtattata tccgtgatag
    12481 ccaccacttc ctccatatcc acctaaacct ggtctaccat ttggacctgc ttctccatct
    12541 ttcactgtta tatcacaatg attaattatt ctattttttt gttcctctga atctgaaatg
    12601 aagtaaatat tacctttctt accagcattt ccacctcttc cagcatttcc accagctcca
    12661 cctttttcac cttttttgcc acttgtacct ttttcaaaat accctttaaa catctgtccg
    12721 tggaaataac ttgtatatcc tgcatcagct ccattacttc cagaagctcc tgtttcacca
    12781 ttttccccat ctattccttt agaaccattt cctccatttg caatcagtgt tagacgataa
    12841 tttctaattt cttctttaat atataagtaa atattacctg catctctacc atcttcacca
    12901 tttttcccat acatagaatt atctacaagt tctccgctct ttccacttac attaattttt
    12961 acattactac ctacatcaag attgttagaa gcaagaataa tatttttctc tgatgcaaag
    13021 ttcattccaa ggttatcatc catataaata gtatctttag caaagaacat aaatgtagaa
    13081 acattgtcat ctacttttct atttttgaaa tattccaata cactgctaat tactaagttt
    13141 ggaattaatg ctcttatttc aatacctttc ttatcattaa tattaattgt ttttactata
    13201 aatggattta aaatgtattt accaataaat tctctaatta tctcattacc ttcttctcca
    13261 tatgcactta atcttgttat actgtcttct aactcttcat tttgtgtaac tgtaacattt
    13321 gtcaaaattg aattaaaatc acttacacta tatacataat cctttttatt tgcaacttcc
    13381 attaattttt ctctatttgg taatgtaaat acctgttttt gcacttcata agaaactaaa
    13441 actttttcta aattctttct tattataatg aagttttgtt ccattatctt cttacttgcc
    13501 ttttctataa taaattccat atgtacagaa ggtaataaaa tttccccagt tccatgttct
    13561 gtatatttac ttaaagcatc aattatatct aagtttcctt ttatcttatt ttgtaatttc
    13621 atgcaactat cttgtaagaa taatccactt tgtttggcta atacgttgat aaatctctct
    13681 atagtaagac ttgttgtact ttccaaccag tttttaaata cttttacaac ctcatcatat
    13741 tttgctattt cttgaatcat tgttatactt ccaataatat tgataaaatc ttctgtatac
    13801 gccttaacat aatcttctag gctagatacg atattgtttt gaatttcttt tatcaacgta
    13861 tctaaaacta atcttgcatc tgcactaatt ggatattgga atgctgatgt cttatcttta
    13921 acttcatcta attctagtac ttgctttaat aagccttccc tctcatctga tgcaagtggc
    13981 ttacaaagat gaatattttt tccattatct tttagcaatt cttcaaataa ccaagctttt
    14041 tcctctatat tactgtctaa attttcgcat aattgttcat attttctttt tataacatta
    14101 tatatttgtt tttctgttct tcctaagaaa ctttttgaaa tgaaaaaagt tatactaccc
    14161 ttaaaatttt ctttatgaag acacatattt aactgttgga acaggtctag tacttttgta
    14221 agtctagaat ctaatatatc tgcttctggt atcaatacaa ttactgcaca tatagatgca
    14281 gaatttttaa atacttcacc taatgaaact gctgcactaa tatcatgggc tactccccta
    14341 ttgtcgctaa aacctgcagt atcacataat tttgcatcca ttccttctat tggttttctc
    14401 atatttacaa tgttcgggta taaagtttga gaaacagctc ctgaaccaat ctctgccaca
    14461 gggtctaagc aatcaatata tgatatacca gtatcttcat cttctacttc catcattaca
    14521 catcctgcaa gatagttaat tgcagtactt ttgccacatc ctgtcccacc aagaattaga
    14581 attaaatttt gattttcagc cttatttatt gctacttttc cttcatttaa taagagtgtt
    14641 atttcttcta aaagttgatt gttattttct ggcatttatt ttcccccttg gttatatatt
    14701 ttatttaaat aaagtttaat cccttaagta gattgcaaag tttttatact atatttaaat
    14761 ttgtttttta ttttttattt ttaataaatt tgcattttca ctaaatttgt attttcactc
    14821 aataattatt cttctttgtt tattaaaata actgtgttgt ataatataat ctttatcaac
    14881 acagttattt tatttaatgc gcttatttta ttgtatttta tgtaatacag ttgtttttta
    14941 tattatatgt ccattatttt cctgtggtta ttacatatag atttttaatt atatttgtac
    15001 cattaattta aacattccta tactactgct taagttttac acctatacca gcttgaatat
    15061 tttattttta tttgcttaaa tagttcaact atactggttt aaataggtta tctttatttg
    15121 cttaaatagt tcaactatac tggtttaaat aggttatctt tatttgctta aatagttcaa
    15181 ctatactggt ttaaataggt tatctttatt tgcttaaata agttcaacta tactggttta
    15241 aataggttat ctttatttgc ttaaattagt tcaactatac tggtttaaat agttttcctt
    15301 tagtttaaat aattcaccta tacctacttg aatagttcac ttttatttgc ttaaattcta
    15361 taccaatact ggcttaaaca ttatactacc atgattgtat tgtctatctt caactacaat
    15421 aaaaacaggt tctaaatctt tagtagatgt tatttttaat tctaaaaaaa cattatcagt
    15481 tggtaatatt tttacatcat aattatttaa attattattc gtgatactat taaatacatc
    15541 accttgacgt tcaaaaaaaa cattatcaac tattacaaaa taaacatctt cttttctact
    15601 attttcaacc tctatagtat tttgtttagt tacatttagt ctcatcttcc acctcctatt
    15661 aatattactt aattatatta atacttgtaa ttgttgcatt atgttcatca caaggaagag
    15721 gtttaaaaac aactatctct gctaaataat tctgtggagg atttgtaact gaagtagcat
    15781 tacaaataaa tttaaaattt gttcctgcta caacttgagt agaaacaata agtggttcat
    15841 aaccaacccc ttctaacatt cctacagctt cattaaatac ttttaaatct tcttcttcga
    15901 cagctctgaa ttttgaccat ccaccaacaa aaagactcat ataaaaacct cctttatata
    15961 tatatttatt tatatatata gtagtaccaa ttttctcaat attcaatatt ttctgtgtaa
    16021 ctggtcaatg aaaatgcata actggataaa aatacttata aaataactta actttacata
    16081 taaatagctt aatttacatg taataagatt attctacaat atgaaataca tcttattttg
    16141 aatatattct aaatacaata atggatattt tcaattatta tacttagaaa atatccatta
    16201 cttaatttgt tttatttaaa atcacataga gactttatta ttttttttat tgcatgaata
    16261 gctatttcag gtatcataag gtgatatacc ccctccttga aaccacaaaa aacataacaa
    16321 actttacgaa tcttgtctta aaagaccaca ggtacattcc ttatttgtgg attggagtag
    16381 ccataacaag caaattttac aatttttatg atgacaaaaa tcaataaaat cgcttagtat
    16441 ataacacgaa atactacttg ttgctattaa gaatagttag ataggaagta tgcattatac
    16501 caataaagga atcaataaag aaagttaaaa catccctaca aattaaaaat ctgttacatt
    16561 tttctatatt taaacgttat ttacttacaa ttggagaaat aacaggtttt ccttctcggt
    16621 caagtaaagg cgtaaatccg gctgcatggc cataatagtg gaatacatag ttcacacctg
    16681 tttctttatc cacccaaatc tccgttgcat ttatttttcc ttgtgaatat actttttcaa
    16741 atctatcttt tcccatttta taatcctcca tatttttgat tggtctatgc cttcttcact
    16801 taagtagaaa aaatacataa cttatttttt taattctaat atgagcacgt aaaaatgtca
    16861 aacagcttat ttaatttcat aacttcatgt tactgttcgt aaccttgtct gtttacttac
    16921 actgcattac agcaccattt tatcctttac attattgtat tgcacacagt aaaaatgctt
    16981 actttattgg atttttcaac taaaaaaagg attttttctc cacagaacat ttactatttt
    17041 ttataatata tctaatttta ctctcatatc aacatttaat gaagatataa cctttttcaa
    17101 atattgtaaa gactccttaa aaatattaga aaaagattct gcaaatctct caacatctcc
    17161 tttttttaaa tacctaagta tctcaaaaca tacatcaaaa cgtgttttca aaatttcatt
    17221 atattcttca tgaaaaaatc tctctaaata gattccaaat atcatatatt gttttaaatg
    17281 taaaaatgtt tcctgtaata cataaatttg tgtataatct actatcttac aaagtaaaac
    17341 aaaatctgga taaaccttac actcttcaca attattttgt aagtacctta aatgagtttc
    17401 tattttatct acagctatca aatccatttt atttcctagt aaaagtacta catcctcaag
    17461 agtaatcgta acaaactcca tagcatccaa aagcaaactc aaccttttag ctccttcttc
    17521 tgtatttata taatcatcat catattcaga aaaaagtgtt atatatgttc ccttgccatt
    17581 aattgtttta gcaattccca attcatttag ctcattatag gcacttctaa ctgttggaag
    17641 agatacatta tatctattaa ttatcgaagt aattgatggt agacaatctc ctattttaat
    17701 attgccttca aaaatttgac aaattacatc tgaaacaatt aagtcatata aataacattt
    17761 ttccaacgtt acagaattaa aactacttgt atctatactt tgtggagcac gattttctac
    17821 cattttaagt atggattctc tacaagcttt atacatatat gttaatagtt ttttaaattc
    17881 aattagattc ccgtccaatc catataagta agtttttctt acctgctcta tgtatttatt
    17941 acgaaaaaag tctatacaac aattatccaa gttatccaag ttatcgaaat tataggccaa
    18001 ttgtgaaata atgacatcat gctgaataat ctcacaaaaa tggtgaatca ggaaattatt
    18061 tgttttactt attagtttat ttatatattt tagcctttgt tctttaaaga ctaaataatt
    18121 agaactattt ttttccatac aatcgataat ctcgtttaat tcttcaaagt ctttagatgt
    18181 aaatctttta atattgtaca tagcaataga aggaaatatt aaactaaaaa tcttatatat
    18241 ttctgatatt tgtaaaatct tctcatataa taagtcagat tctattgatt gtttactcgt
    18301 actctggatt aaaactggtc tagaaccagc ttttgtctct ataaagccct tttctgagag
    18361 catttttatt acagttttta tagtattttt tgataccata tattcttctg acatgctttt
    18421 tatagaatca agcctatctc caatagtaat tttaccagta taaatttgtt gaattatatg
    18481 ctcaaaaata attaaatatt gtggttttct cttcatattt ttcctcttcg ataaattaga
    18541 tttattgttt tttgtaaggt atatttactt ttatttaata gggcaaaata gttgcttatt
    18601 ttttcctcat aatctataat taatatagac catttatata tatggtctaa gttttataat
    18661 actgtaatgc agtaaaatag catattgtag ctatactaca gcaaattaac ataccattgt
    18721 agtatattaa ttggaataat tttaagtact tcaaaattcc agcaaaacat atatgtgttt
    18781 ttgatattat gtatattcgt taatagaata gctatacttg ttttacataa taaattatat
    18841 gatacttgct atatgtaatt actatattta atacacataa ctattatact tactatatgt
    18901 aattactata tttgatacac ataattatta tacttactat atataattac tatacttgga
    18961 cacataacta ttatacttgc tatatgtaat tactatactt tatacacata gttattatac
    19021 atgctatata taattactac acttggacac ataactacta tatttactat atgtaattat
    19081 actatacgtg gacatatagt tatcatacat actatatgta attactatac ttggacacat
    19141 aactattata tttgctatgt gtaattatac tatacttgga catatagtta tcatacatac
    19201 tatataatta ctatacttgg acacattatc atacatacta tatgtaatta ctatacttat
    19261 acacatagtt atcatatata ctatatataa ttactatact tggacacata gtcatcatac
    19321 atgctatatg taattactat acttatacac ataactatta tactttccat acataattac
    19381 tatatttgga cacatagtta tcatacatac tgtatattaa gatataatac tttttaaatt
    19441 ttatataaca cagacacatg caataaaaaa gaaggtatcc ataataagct ctttgcatgt
    19501 acctaaataa aaactgtaaa atagaatata taaaatctaa tctattttaa gtaaatacaa
    19561 tttcacttta aaagaggatg tactatgaaa taccatatat ttttaatcat aactactata
    19621 ttaataatcg aactatgtaa catttacata tgcaaaatag catcttttag aaacaaaaat
    19681 ataactatac ataagaatta caaatatatt tttttaataa aaccattaac cttcttgata
    19741 ataatattca caaaattacc attaccacta tattatatat tgttttacat agtaagtcat
    19801 aaattgctct taagcagaaa aagttctact aacaaccatt ttattcccaa tatggcagta
    19861 ataagttttt gtattttaca ccttattact gtaagtacaa tatcacttgt taaaaataca
    19921 agtccagata taatatttgc aaatatgacc tataagttaa ttagtatttt aatagcttct
    19981 attttagatt ttatcttatg gtactctatg aataaaataa aagagcatat ttatataata
    20041 actcaaaatc aaatagagtt cagtctattt ttaaaattta tctggttcac tttaggatat
    20101 atattatttg atagtataca aattacattt gctccaccaa gttctctaac ttcctacttt
    20161 atattgggaa gtaacatatt attaatctta caactaatta tattcatcaa tcatatatat
    20221 tccattgaaa aaaatattta tttggaaaaa gaacataata gactaaaaat gataaaacaa
    20281 gaacaattac taaaagtaaa tttattaaag aaaaaagcca acattgatga attaacatca
    20341 gcatataatc gaaaatttgc ttttgaaaaa atacagcatt acattgataa caatttatta
    20401 ttttcattga tatatatcga tttggataaa ttaaagacga taaatgatac atatggacat
    20461 ttagctgggg ataactatat tattaacttc tcaagtatag ttattgaaaa cttaggaata
    20521 gatgacatat ttgctagaat aggaggagat gagtttatag ttattatgcc taaatctaat
    20581 gaaaacacca caaaagaatt tatagagaaa ataagtaaaa ttatggataa tacgatatct
    20641 tttagctatg gtattgtaga agtttctagc gaaaacaaaa tgactgtaga agaaattatt
    20701 aatattgctg atattagaat gtacactaat aaacaaaata atcataaaag gaacaataac
    20761 taaaatgaat atttacatga gcttacttgt agtctcacta tgttttttat cttattatct
    20821 ctatcattat aattttttta aatatctgga cataatgatt aataactcta aaaataaata
    20881 cacagtatat attatatatt ctatatttaa ttatttaatt tttattattt gtacttattt
    20941 aaagtttcat ctaattataa attggacaat ttttatgttg attttattca tacaaattaa
    21001 agtaacttat aaaatctcca caataaaaaa cattttttat tcactaaact gctccatcat
    21061 tggtttagca ataaatatat tttttaggtc tttaatgtca atagtgttaa atatgccagc
    21121 atatttattt gatagtaatt ctattgcaaa tatttacaga gtatttccta tatcatttag
    21181 ctttatagtt acaggactat atttcagtta taaaaataat aaaaataata agattaactt
    21241 tgaggcagtt ttagaggaca aaaaaaactt aattttctta ttgagcctta tgtttggaat
    21301 gtacttgtat ctatgtttga gtttgtcaat atactatact gttggaaatg tattgatttt
    21361 aaaattatat ggaatcaagt ctgccataat agttttggta ggtaactata ttgcagaaag
    21421 atatagcttt agaattactc aaataaaagg ttatgatatt aaaaatcaaa tagaatatgt
    21481 agaaagtgta aagacagagt atgagattaa taaattgaat gatattgtat atacagattt
    21541 actgacaggt ttctacaata gaccttttgt gaatcaaaaa ctatcagaat ggacatcaaa
    21601 tcactacaaa tttaccttat gttttactga tttaaatagc ttaaaacctg taaatgataa
    21661 atttggacac caatttggtg ataaatacat atccactact gctcaaataa taaaaaaact
    21721 tataaatgta gaaaattcct tactatttag atatggtgga gatgaatttt tattcctttt
    21781 acagaataca agttgtgtag aagcagaaaa gataatgatt aatataaatt atgaattatc
    21841 aaaaataagt aataccgacg agtatcctta tgctctttcc ataagctatg gattagttga
    21901 gtgggatgga atatccgata ttgaaacttt aatatcaaag gcagattctt tgatgtatga
    21961 aaataagctt aaaaataaat ctactttaaa ctagctagtt cagatattct acaaaatata
    22021 tattataaga tagtactaag aatgtcaaaa tatatagatt ttgcaaatat tgaatacagt
    22081 ttactaatga atgcactgga tgtaagtgtt agtaaacacc tattagatga acactttact
    22141 gttgtttggg ctaatgaccg ttattatgaa atgttcggct atacctagct aaaaagttaa
    22201 aaatgaccac tgtagccgag ggaatagaga ctagttttca aaaagatttc ttgcaagaaa
    22261 tagactgtga tatgttacaa ggatacgtgt tctctagacc tttaccaata aaagactttg
    22321 aaaaacttat gtttcaaaac tcctccaatt aacaatataa aattgcttat tataagtgat
    22381 attattgtat caacaaataa aggaattgtc tcaaaattga ttttttctaa tcaaattttt
    22441 attttgagac attctcattt ttttgcataa gaaaataccc accaagctac ggtttttctt
    22501 accgctgttc ggtgggtatt agtagtatta tgaaatctga aaactttaag tattactact
    22561 tccgctagcg caatcacaaa ggtcgaaagc ttcaaagcga aaataggctt tttactcatc
    22621 aaacgtcacc gtacctgcca acccgtccac cgttatcatt tgtccgctct taatagctcg
    22681 tgtggcaatg tgtgtagcca taacggctgg gatgccatat tcacgtgcga caatgcttga
    22741 atggctaaga ggaccaccaa tgtccgttac aatcgcactt gcagatgaaa ataatggtgt
    22801 ccatgctgga gttgtggtaa ctgcaaccaa aacacttcca ggttgaaagc tttcaaaatc
    22861 tgctggacta ttcagtacac aagctggagc cgtcacaaca ccagtactgg taccaatgcc
    22921 cgtcaacaca attttcccat ctttctgctt ctgttgtgct tgtgatatag tttttttgtt
    22981 tttctctggc aacttggatg gtgacacata acccatgtat tttttaagtt cagcttttcg
    23041 aacaggtatt atcccgttca tatcagatag agacttgttt ttatccaact gctctataag
    23101 tacctccaat tccgattttg tgagccaata aatatcatct aaatgtgaaa tagctcctcc
    23161 tcgtatgaat cgttctgaga tttcctgaaa catacaacga atcagaggat gtcccattcc
    23221 catcaaataa atagcatttt cacgcatagg agaggtttcc tgtgcccaat ggagcaactt
    23281 aaaaaatagt tttttacgca atccacctat gtgttgtaaa atctcttctt ctgcatgttt
    23341 tctacgcttt tcaaacgtgg tctggcgtaa aaaaggactt tccccttttc cttccacaaa
    23401 tgtttttacg gattcaaaag ttggtgtaag tgtttcctgc ggtgtggaat gcgcaaaatc
    23461 aaactcataa gcggtacgac caaactcttt aaagtactta ttgattcgat ttttccattc
    23521 tatccatact tcctgtgaaa ctccaacagg cacaactgaa gacatgaaat cttctgctat
    23581 ctttattgca ggactgtttt gcagataaag gttgatgctg ttattttgct ttacccattc
    23641 tgaaataccc cacaactcct tttccgattg cagaacaatc gtatcaaacc caagcaggaa
    23701 aacggaagta tccgttatac cagcacgtcg aactgctcct tgaaataatt ttgtaaataa
    23761 tgtttcactg atagaagcag caggcaatgt aagctgaatc cttgtgaaat aaatacatgc
    23821 ggcataaaat attgtctgaa taccctccat aatttgatgg gcgttcatag tatgcagggg
    23881 cttttcttcc cattgtttaa tagcatcctc atatcctttt cgtgccgttt cccagcgtgc
    23941 aacgctgttc ataagtgtcc tacgtaaaga gcgtggggaa agggatttga tagcaatcaa
    24001 aagtggattt gatttagcag aaagataaac atatccatta ataaccgtat atgccccgtt
    24061 ttttggtaat aattttttcg cacttttacc aaacatatct atccataaaa gctctgatgc
    24121 acggtttacg atttcaagac caagcgtggc gaacaaaggt gtaacaggat ttggcaaatg
    24181 ttctgccaaa cttccctttg tgtatacaac atcctgttct ggcaataccc attctggtgg
    24241 cagaacagta attggacgag cctgaacaat gtacaattta tctttttcca gtgcccactc
    24301 tacatccata ggcatttgat aatacttctc aattttcttt ccaagctgta tcagttgcat
    24361 tacctgatta cgagtaagag catatttttt tctcaacctt tcaggaacca tggtttcttc
    24421 tgttccttct gaagtacgaa ccgtcataat ctctttattt gccacttcat acgatataat
    24481 tcgttcggag tccttatcca ctacgattgt atcgggtgta actaagctag aaaccaccgc
    24541 ttcaccaagc ccccatgcag cgttaataat catttcgcta cgtctaccat tgattgggtt
    24601 taacgtaaac ataataccag atgaatcgga aaatgcaagc ttttgtacta caacagcaag
    24661 tgcgacaatc tcgtgcttta tattattctt cacacgataa gcaatagcac gagctgtcca
    24721 taaggaagcc cagcatctct ttacagagtc aataacttta tcttcacctt gtatgttaag
    24781 ataagtttcc tgctgacctg caaaagaagc gtcaggtaaa tcttcagcag tcgcagacga
    24841 acggacagcc accgatatgt tccctaatcc agcataagcc attttaattg cgtccgacac
    24901 ttcctgtggc atttctccat tatggaaaag ctcccctatt ttcttagata catcttcaag
    24961 ttggctggta ttattggaat caataccatc tagcagttta ttaattcgag actgaatatg
    25021 gttcgtctca acaaaaatct ggtatgaagc agtcgtaaca tgaaaaccgt ctggcacagg
    25081 gatgcctgct gtcaatagtt ttgataaaga catccctttt ccaccaacaa tttcaatggt
    25141 tgcttgttta tgttccagtg gcaagacata ataaatcata atcattcacc tccatcattc
    25201 ataattccgt gcagaaatat attcattgtt tcatccaaca ttttttccga actatccaat
    25261 ggtcgtgacg tgtagctgat tgataacatg gagttaatta acaaacgtgt cacaagagcg
    25321 gtatccgttt tgcgaaagat gcctgtggtc acaccattat caattaccga ttttataata
    25381 tcttgatatg cataacgagc atctttaagt ctctttcgat actcttcttc taaataatca
    25441 gattctgtat ttaaccacat cagtacagtt ctatactgct ctggaaatct aagatgatta
    25501 agcatgattt ttctaaggca ctgttgtggt gaaggttcac catcaataat cctatgaact
    25561 tgttggatag ttttttctat ttctttctca actgcatata caacaatctc gtctttcgtt
    25621 ttgaaaaaat cgtatagact tgactttccc atgttggcta aaactgcaat ttctctcatt
    25681 gatgtttttt ggaatccatt ttcagccata agactcaaag caacagcaac tattttttct
    25741 cggcgttctg cttgttgttc gggtgtaagc acaattcctt ttggcatagg gcgatacctc
    25801 cttttagcga ccactggtcg tttctttttt atttatcata cagcgaccaa cggtcacttg
    25861 tcaaggttaa tttttttcat tgtatatggt actcgagata aaaatgttca ttttagaaat
    25921 tgctctattt aagtagaaat aggcttcatc attagaaaac aagactataa atttgagata
    25981 tttatcatgt tcaaatttca tcaatatcaa agtttaagat attaataaaa aaagaattac
    26041 taagaaataa catataaaga ttcaaaaaag gagttgaact taaaattcaa ttacaaaaaa
    26101 tcaaatttaa gacaactcct ttattcttta tctctactat tctttatctc tatttattta
    26161 ttacaactcc ctaacattta ccactactgc cttaggtcta gtcatagcct cgattgaata
    26221 cttaacacct tgagttccca taccagatgc ctttacacct aaaaatggga agtggtctgg
    26281 acctctttca gtcttattat ttatttgaac agtaccaact tcaagtttat cagctatata
    26341 aaatgcctta tcaatatcac ttgtaaatac tgatgattga agcccatact ctgattgatt
    26401 agcaatttca attgcttgat ttatatcttt aactcttata attggaagta ctggaccaaa
    26461 tggttcttcc caagcaattt tcatatcaac atttacatta tctaacaatg ttggatagaa
    26521 taaattattt tttctaacat tgcctgttat taactttgct cctttatgta atgcatcatc
    26581 aaccaaccct tgaacaaaat cagttgcttt attatctata agaggagtga taactacttc
    26641 atccattgga tttccaattg tcaacttctc taccaatggc tttatttttc cgactaattt
    26701 atccgccaca ctctcttgta ctaaaattct ctttactgcc gtacatcttt gtcctgaata
    26761 tgaatacgct ccagatacta tattcttagc tgcaaaatct aagtctgcat cttctaatac
    26821 tatagccgca tcttttccac caagctccaa taataatggt accattccag atattttaga
    26881 tatatgctga ccaacttcag tactaccagt aaagttaata aaattaattc ctttatgcgt
    26941 tacaacataa tctcctatct cactacccct acctgtaata gtattaagta ctccatctgg
    27001 tatcccagct tttctcataa tttctaccac atgaagtgcg ctaatagccc cttgtgtagc
    27061 aggttttaat acaactgcat ttccaccaat aagtgctgga gcaatctttg acattgataa
    27121 atttacagga taattaaaag gagatatagc taaaacagta cctaatggaa cccttgaaac
    27181 ataagacatc ttatttcttg ttccaccagg gaaattatct ccactaatgg cttcaccctc
    27241 catatttttt cccacatcag ctgtgtatct taaaaaatct gctgttctct caacttctgc
    27301 ccttgcagat tttatgtctt tggctatttc aagtaccagt atattagcta tctcatctat
    27361 attttctaac aataaatcag cagccttatg aaatatattg gctctcttat atacaggcat
    27421 ttcagcccat acttttatac tctcttttgt gttttttata acctcatcta cctcatgttt
    27481 tgtcatagat tgtactttcc caacaagaga attatctatt ggtgagtaaa tttctattgg
    27541 ctttctcgaa ttagattcta cccattttcc atttattaaa tttttatata cattttcttt
    27601 tgttttaagt tcattaaaca tatttttatt cctctctctg atataacttt gtgtatttaa
    27661 attatagcca tttattcgtt atgtaaacat ttcttttttt cataaacgtt tacctataaa
    27721 aaaattttaa gttaacgttt ttctacattc ttctatttta ataacatact cgctccaaat
    27781 ctatataggt cacactttaa tatcaatata ctaatatacc aatataccaa tataccaata
    27841 taccaatata ccaatataaa tattataaac ataaatcact tgacaaattt ataagtagac
    27901 aatattatat atctatgact taaaatattt ttaaagtcat agatatcata tatagataga
    27961 aaggattgat aaaaatggct aaaagtttta cagaaataga aaaagacaat ataagaaaaa
    28021 gactaatttc agaatgtgaa atcaactggt ctaaatatgg atacaaaaaa actaatattg
    28081 atgaactttg ttcaaaatca ggaattgcaa agggttcatt ctacacattc tttgattcaa
    28141 aagaaaagtt attttttgaa gtattaaatg gaatccaaaa cagacttata tcatcagtag
    28201 atgaaatttt aagtaacatg cctccaaaag aaggctttgc aaaagcctta aagatgttgt
    28261 atcgattata tgataaaaac ccattcttat attcaccaaa taatactgat tacatcactt
    28321 tattgaataa gatacctaaa gagatgcttt cacaacttga atacagcaat gtacaaagtt
    28381 tttatagctt aattgaaaga cataatctca agttaaaaat agagaaagaa aaagcctttg
    28441 gtgtttgcaa tgccctactg tcaagcctat tgctaaaaga agatattgga tatgactaca
    28501 ttgaagtatt tgattttatg gtagacaatc ttgtagaaca catacttgag taataattta
    28561 aaataaattt tctaaacatt tctttataac taggaggagt aatatgattg aattaataca
    28621 aagactaaat gatattattt acaaaataaa tgataaacta aatatccaca caaaacttgt
    28681 agaccctagc agtgagctca tgccacttta ctctatgtta tctataatcg tttctatatt
    28741 tctaataata ttaggtatta aagtaagaaa aaaattcctt ataataattg ctattatatc
    28801 aattattata aatattttaa taatcttaga taggtaggta catatatggt aaaatcctcg
    28861 aacactttta ataaaaactt caacttgatg atattaggtc aaataatctc attgtttggt
    28921 gcatcaatac ttaaatttgc tttatcttta tacatacttg atataactgg taaagcagaa
    28981 atatttgcta ctatactagc agtatcttca atcccaatta tagtattctc ccctattggt
    29041 ggagctattg cagatatatt caacagaaga aacttaatgg ttatttttga tttttcaagt
    29101 agtcttacag tactaatctt agcagtattc ttattcaaca ataatggttc aatattactt
    29161 gtaggaatta ttatgaccat attatcagtc ataagcacaa tgtatcaacc aactgttcag
    29221 tcaagtacac cccttttagt tgataatgag catttaatga atgcaaatgg tattgtagct
    29281 ggagttgcat ctcttacaaa tattgcagga cctgtattag gaggtgtctt gtatggtgtg
    29341 attggaatta atgcaattat agttataagt tgtgtaagtt tctttctatc tgcaattatg
    29401 gagatattta tacagattcc ttttatcaaa caagagcaaa acagcaacat tataaaaact
    29461 atatttactg atataaagga tggtattgtt catatatgta aagaaaatag atttatacta
    29521 aaaatacttt ttttagctat agcaataaac ttatttttaa gctctatgtt aatagttggt
    29581 atcccctata tagtaaaaat cactatggga gctagtaact tcttatatgg tctagcagaa
    29641 ggagcaattt cttttagttc actggtagct gctgttagta ttggtatttt ctcaaaacat
    29701 ctaaaaatca ctaatttgca tatttccttt attatatgtg cagttatgtt cataccaatg
    29761 gcggtttcag tatatccatt tatattaaac acaggatttc cagttccatt tttaatcttt
    29821 atagtatcat ctatgctaat attttttaca acgactctta tctcaatata tgttataaca
    29881 gtcacacaat taaaaactcc aaatgaactc ttaggaaaag taatgtcaat tctttttgca
    29941 ggaacagcaa ttgcaatgcc ccttggtcag gttatgtatg gtaaactttt tgatttatta
    30001 tcagatagag tgtacatcat tatactagga gttggattta ttacattact tatagcattt
    30061 gtatcaaaga taacattgag gaacgaaaat ctttagatac aaagaattta gatataaaga
    30121 tttaattttc actaatttct gaatttaaat gcaaaattgt gtatcagtat gttgacgttt
    30181 tagaaattga ttgatataat caatggaaaa taaactttat aaaaattcaa aatattcagc
    30241 tagaggagtg ataaaaatga gttacatgcc aactttagaa caagcatggg aaatattaag
    30301 aaaatacaat aaggaagaat ttcatataag acatgctcaa attgtatctg gagttatgag
    30361 atactatgca aaggagtacg accctgaaag agtagaattt tgggaaatag ttggactttt
    30421 acatgattta gattttgaaa tgtatcctga tgaacattgt gttaaacaaa gagaattaat
    30481 gacagagctt gatttagata aatcaataat cgattctaca ataagtcatg gatatggatt
    30541 aactggttca gatgttaaac cagagcaatt tatggagaaa gtattatttg ctgtggatga
    30601 acttactggt ttaattggtg ctgctgcaat aatgcgtcct tcaaagagcg tttctgattt
    30661 agaattaaaa tctgttaaga agaaatttaa agataaatca tttgctgctg gctgttctcg
    30721 agacgttata agaagtggtg cagaaatgct tggatgggaa cttgataagc ttatccaaac
    30781 aactatagaa gctatgagaa gcttgattcc agagatggca atatagctcc tccatacata
    30841 taccatagaa gttacatctt taaagagcat ttctcttttt gatgcaactt ctttttatat
    30901 tttgtagaat aaaaagttta atattttaaa ctcactatat aatctataat aaatactata
    30961 aataatataa tgtataatag tcataaactt tctaacctta aaagtttcta ataatcaat
//
LOCUS       DAMJWS010000044        30700 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMJWS010000044 DAMJWS010000000
VERSION     DAMJWS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.044"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            165..2066
                     /locus_tag="PC399_002111"
     CDS             165..2066
                     /locus_tag="PC399_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2592968.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2069..3112
                     /gene="ypdE"
                     /locus_tag="PC399_002112"
     CDS             2069..3112
                     /gene="ypdE"
                     /locus_tag="PC399_002112"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2592969.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3127..3432
                     /locus_tag="PC399_002113"
     CDS             3127..3432
                     /locus_tag="PC399_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2592970.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3529..3864
                     /locus_tag="PC399_002114"
     CDS             3529..3864
                     /locus_tag="PC399_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2592971.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3896..5218
                     /locus_tag="PC399_002115"
     CDS             3896..5218
                     /locus_tag="PC399_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2592972.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5228..6298
                     /locus_tag="PC399_002116"
     CDS             5228..6298
                     /locus_tag="PC399_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2592973.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6303..7490
                     /locus_tag="PC399_002117"
     CDS             6303..7490
                     /locus_tag="PC399_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2592974.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7687..8559)
                     /locus_tag="PC399_002118"
     CDS             complement(7687..8559)
                     /locus_tag="PC399_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF2592975.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8953..9144)
                     /locus_tag="PC399_002119"
     CDS             complement(8953..9144)
                     /locus_tag="PC399_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2592976.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9364..10410
                     /gene="cobT"
                     /locus_tag="PC399_002120"
     CDS             9364..10410
                     /gene="cobT"
                     /locus_tag="PC399_002120"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF2592977.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10442..11002
                     /gene="cobU"
                     /locus_tag="PC399_002121"
     CDS             10442..11002
                     /gene="cobU"
                     /locus_tag="PC399_002121"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF2592978.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            11012..11785
                     /gene="cobS"
                     /locus_tag="PC399_002122"
     CDS             11012..11785
                     /gene="cobS"
                     /locus_tag="PC399_002122"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF2592979.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11805..12419
                     /locus_tag="PC399_002123"
     CDS             11805..12419
                     /locus_tag="PC399_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2592980.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12486..12665
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12795..14294
                     /locus_tag="PC399_002124"
     CDS             12795..14294
                     /locus_tag="PC399_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF2592981.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14333..15709
                     /locus_tag="PC399_002125"
     CDS             14333..15709
                     /locus_tag="PC399_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF2592982.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15710..16681
                     /locus_tag="PC399_002126"
     CDS             15710..16681
                     /locus_tag="PC399_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2592983.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16713..17783
                     /locus_tag="PC399_002127"
     CDS             16713..17783
                     /locus_tag="PC399_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2592984.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEEREYML
                     QELRNIRNIKVYDTDANFILIKIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17785..18672
                     /locus_tag="PC399_002128"
     CDS             17785..18672
                     /locus_tag="PC399_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2592985.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18660..19292
                     /locus_tag="PC399_002129"
     CDS             18660..19292
                     /locus_tag="PC399_002129"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF2592986.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19301..20527
                     /locus_tag="PC399_002130"
     CDS             19301..20527
                     /locus_tag="PC399_002130"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF2592987.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20524..21132
                     /locus_tag="PC399_002131"
     CDS             20524..21132
                     /locus_tag="PC399_002131"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF2592988.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21122..21697
                     /locus_tag="PC399_002132"
     CDS             21122..21697
                     /locus_tag="PC399_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF2592989.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21690..22442
                     /locus_tag="PC399_002133"
     CDS             21690..22442
                     /locus_tag="PC399_002133"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF2592990.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22426..23547
                     /gene="cbiG"
                     /locus_tag="PC399_002134"
     CDS             22426..23547
                     /gene="cbiG"
                     /locus_tag="PC399_002134"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF2592991.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYHVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23544..24269
                     /gene="cobJ"
                     /locus_tag="PC399_002135"
     CDS             23544..24269
                     /gene="cobJ"
                     /locus_tag="PC399_002135"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF2592992.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24266..25018
                     /gene="cobK"
                     /locus_tag="PC399_002136"
     CDS             24266..25018
                     /gene="cobK"
                     /locus_tag="PC399_002136"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF2592993.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            25037..26515
                     /locus_tag="PC399_002137"
     CDS             25037..26515
                     /locus_tag="PC399_002137"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF2592994.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26545..27450
                     /gene="hemC"
                     /locus_tag="PC399_002138"
     CDS             26545..27450
                     /gene="hemC"
                     /locus_tag="PC399_002138"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF2592995.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSAIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27440..28939
                     /gene="cobA"
                     /locus_tag="PC399_002139"
     CDS             27440..28939
                     /gene="cobA"
                     /locus_tag="PC399_002139"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF2592996.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28956..29921
                     /gene="hemB"
                     /locus_tag="PC399_002140"
     CDS             28956..29921
                     /gene="hemB"
                     /locus_tag="PC399_002140"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF2592997.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30074..30652
                     /locus_tag="PC399_002141"
     CDS             30074..30652
                     /locus_tag="PC399_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF2592998.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12439 a         3059 c         5666 g         9536 t
ORIGIN      
        1 attgaactat atattttatt attataaaat atttatatta aattgtggaa ttaaaatata
       61 ctaggatata aaattcttaa ataaaaagaa aagattgttt ttataaaaaa tattttttat
      121 accacttaaa aaaacacaat aataacactg agggaggtgg ggatatgaga gaacgcatta
      181 ttcttgaaac attaaaaaat agtagtggtt atctaaacat agattatttt gcagataagt
      241 tgggggtatc tactcgaact atcagaaatg aaataaaaaa gatagaaact ataaaagaat
      301 gtaatggttt taaattagaa tataagtcta aattaggata tattctaaat attaaagacc
      361 aagataaatt cgagcattat ctaagaaact taccctcaga tttagttgaa aatccagaac
      421 aacggttaga gtctataata gtagaacttt tagtaaatga aggttataaa actatagaac
      481 agttaagtaa aaaatttcta gtaagttcat cccaaataaa aaatgattta aaaaagatag
      541 atgaaaagat aaaggataca gaattaaaac tagagcgaaa ggctcattat ggaattaaaa
      601 tagaaggttc tataaaagaa attcaaagta tattggtgga tagttatttt agagggaata
      661 gaaatataac agagtacagg aataagttta tagataattt aaaattagct aatattagaa
      721 gtacaataaa aaatgtactt aatgaacatg atttagaagc aaatttaaca gaacttgagg
      781 aaatattagc acaaattatt atattgtata taagagttag tatgagagta gcgggaaatg
      841 ctagcgattt aaaattatat acagaagatt taattataga taaattatta gataaaattt
      901 ttagagataa aaaatataac cttaattatg atgaaaagta ttatttaaag gagcttataa
      961 aactaaagac taaagataag aaagccacta taaagaatat tgataagaat aaacttcaag
     1021 atattatgtt cgaatttttt agagagatag ataaaaaata taactctaat tttttagaag
     1081 ataaagagtt ctttaactta ttttacttac atattgcatg tttaatagaa cgtataaaga
     1141 agaaccacaa gataataaat cctttttcag ttaaaataag tcaacagtat ccaactgttt
     1201 ttaatttatc tattcaattt tcaaaaatca tagaaaatga gtatcatata aaaataagtc
     1261 aagatgaaat agggtttata gctactcaca ttgctgttcc ttttgaaaag agagaagaag
     1321 ctaattttaa taaaaaatat aaaatagcaa taatatgctc ttctggtgga ggtagtgcat
     1381 ttttgataaa tttaagactt aaagaaattt ttccaaatgc tgagataaga aatttttcat
     1441 tactagaaga aaaagctgta atagaatttg caccagattt gattttttca ataacaaatc
     1501 ttttatttga aataaatgct ccagttatac ttataaatga aattttagac gaattggatt
     1561 atttagatat taaagaaagt gttagatttg cagaccatat aggaaatata tcaagcccaa
     1621 aacaatatat attaggtcta tttgataaga atcactttag gtgtataaaa gataaagcta
     1681 agtataagga tatccttgat agtatgtcaa aatgtatagt tgacgagggc gcttgttctc
     1741 caacctatcc caaagatgta tgggaaagag aaagctattt gagtactatt tatacaaatg
     1801 gggtggctat acctcatcca atcgagatga caggaaacaa aaatataata tcagtagctt
     1861 tgatacaaac tgatattatt tatgaaaata gggtgcctaa aattattttc atgatttcat
     1921 taataaaggg aaatttagaa cttcataagc aaatatctaa atatttaagt aagattatgg
     1981 caaataagga tatggtagac atgctaaata agtcacaatc ttatgaagag tttatgtata
     2041 agttaaaaat ctatctggga ggttagacat gaatatagag ttttttaaga ggttgtcaga
     2101 agcagatggt atagcatcta atgaagaaga ggttagacat gttttattgg aagaactaaa
     2161 agcttatagt gataaaatta tctgtgatgg gctagggagt atcatctttt ctaaaataaa
     2221 agatgagagt gcaccaaacg taatgatttg tgctcatatg gatgaagttg gatttatggt
     2281 gagaagtata gacaaacttg gtatgattca cttaataaca ataggaggag taaaacctct
     2341 tgcacagttt gtacaaaaag ttagaattac aacaaaagaa gggaagaaaa tccctgctgt
     2401 cataaatgct acatataata atggtaaggc tgaaaatata tatgcagata ttggagcata
     2461 tacagaagaa gatgtatata acctagggat taatgttggg gatatggtaa catatacaac
     2521 aagctttgag gaatttactt taccagaccg attagtggga aaggcttttg atgataggat
     2581 aggatgtttt gtgatgggag aagttttaaa agaacttagg aaagaaaatc taaattgtaa
     2641 tattcacttt gcggctacaa gtagtgaaga ggttggaata aggggagcaa agacatcaac
     2701 acagttaata aatccagata tagtatttgt aatagatgta gcatgtgcta aaaatgagtt
     2761 tgtgagagac tatacaaatc agaaacaaat tgataaaggt gtaatgctaa tgcatagaga
     2821 taggactcta gtccctagta gaaaaatgat agattatgta atggaaattg ctgataaaaa
     2881 taatatacca cttcaacatg atatgtttga atcaggtggg actgatggag gagaagcaca
     2941 tctcgtaaat gaagggaaac cttgtgtagt aacttgtgta ccagttagat atggtcattg
     3001 tgcattttca atagtaagta ataaagattt agagaacatt ataagattgt atacacagtt
     3061 aattttaaat tttgatgaag aacaatataa acatataaga aattttatct aaaggagaat
     3121 aagaaaatgt caacagaagt acaaataatg gaacttatat caactgcagg agagagtaaa
     3181 tcgaaagctt ttgaggcttt aaaaaaagtg aagtcaaaag attttaaagg agcaaaattg
     3241 ttaatagagg aggctcgtaa aatagatatt gaagctcaca ggatacaaac agaattaata
     3301 actcaagatt taaatgaaga tgttgaaagt ccagcaatga gtcttttaat ggtacatgct
     3361 caagaccact atatgacttc tcagctagca aaagacttga tagaggcttt gatatattgt
     3421 cttgataagt aattgtaata tagaaataga ttatttatgt aaaaattctt ttaaaagttt
     3481 agatgctttt aagagataat aaaaatacaa actttaggag gaattattat gaaaatatta
     3541 ttatgttgtg caggtggatt ttctactaat atgttaatgc aaaatatgaa aaaggtggta
     3601 aaagagagtg ctaaactcaa tgaaaaagat tttgagttta cagctatacc agcagattca
     3661 atagaaagtg aagttgatga ttgggatata gtacttatag gtcctcaaat agctcacaaa
     3721 atagacttct taaaaggaat attagagcca aaaaataagc cttttgcagt aatagataaa
     3781 gatgtatatg gaaaaatgga tggagctaca gttatgaagt tggcattaat aacttataag
     3841 aaacatcaat tagctcaagg ataataaaaa taatgaggga aaagggagat tagatatgtt
     3901 ttcaaaattt gaagcattta tgaatagagt atttatgcct ttagctcata aggttgacaa
     3961 tcaacgtcac cttggttcta taaaggctgg tatggttgct atgacaccat tcacaatatt
     4021 aggaagtata tttgcaatac ttccagcact tccaaatatg cttggagaaa ataatccaat
     4081 aagcaaattc atacttaaca attcagcaat attggattta ccagtaagtc tttccatagg
     4141 tttaatagga ttatatgctt gtatgtgtat tgcttttaat ctggggaact actataaatt
     4201 atatatacca ggttgtgttg cactatcaac tttttctttt ttattcctag tagcatcatt
     4261 tacaccagat ggagctttag aaataggaaa tttaggagct aaagggctat ttacaggtat
     4321 gatagcaggg cttggttctg tggagttata taatttttgt aataagaaga atatcacaat
     4381 aaaaatgcca gaaaatgtac ctgattttgt atctaggtct tttgaattaa tacctactac
     4441 tataatagtt tgtggaactt ttatagttgc tcgttttatt tcattaaaag tatttggaga
     4501 attacctcca cagatactta ctcaattttt atctccatta gtaggaagta tggataaccc
     4561 atggtcagtt cttgcactta actttgctat atgtactata ttcttttttg gaatacattc
     4621 atcagtattt agtcctataa ctagacctat aatgattaca tttatagctg aaaacatagc
     4681 agctatgcaa gctggagaaa caatacctca tttctttaca gcaggagctt caagtgcatt
     4741 ctttggattt acaggatgtg gaattagtat aggttgtgta attgcatgta tgttatcaaa
     4801 aaatgcttcc tataagaaga ttggacgtgt atcattattc cctgcattat ttgggataaa
     4861 tgaaccaata ttatttggag caccgataat attaaatcca ataatgttta taccacatgt
     4921 atttggtgga gctataatag gtacactacc aatgtttttt atgcactggg gatggttagc
     4981 aaagccagta tttgaccctc catatgtagg ggtattctta gaaggatttt taactaatgg
     5041 agattggcat tctatattag caaatgcatt gcaattagta ttagcagttt tattatattg
     5101 gccattcttt aaaataatgg aacgtcaaca aaataataaa aaacaaaaag aagaaactaa
     5161 gaatatattc tctaaagaag aagaagactt attggatgat ttaggattgg atttctaata
     5221 ttttgttatg aataatacaa tggagagatt agtatcctta atctataaaa gaggtgttga
     5281 cgctcttttg attaaagcaa aaaatactaa aagatatata ggagctttat caggtagtgg
     5341 tgtatatgtg cttgttacta aggataaaaa ttatcaaatt ctagatggca gatatattga
     5401 tgaagcagat aaaaaaacga ctggtttcat aaaaaggatt gtatctcaag gttcttatat
     5461 accaactata attgagttat taaaagaatt aaatataaaa aagttagcaa tagaaaacca
     5521 agctatgagt atacaagagt atctttcact aaaaaatgaa ggttttgaaa tcaatttaat
     5581 aacaaatgaa ttatggaagc taagggctat aaaatctaaa gaagaaattg aacttattaa
     5641 aaaggcttgt gaaattacag atgaagtatt tgatgaggtt atttctgaaa ttaaaaaaga
     5701 tatgactgag ctagaagtat cagctctaat tcagtatcat gcattaaaaa aaggtgcaag
     5761 tggaatgtct tttgaaacaa tagtggtatc aggagaaaga ggtgcaatgc cacatggaag
     5821 acctacaaac aaaaaattga aagtgaatga agctataaca atagattttg gtgttgttta
     5881 tcaagggtat caatcagaca tgactcgtac aatatctata ggtaaacctc ctaaaattat
     5941 taaagagata tatgatgtag tattggaagc acaattatct gcaatagagt caataaagga
     6001 aggaactagg gcaagtgatg ttgacaaagt ggctagaaaa attatagata aacatggatt
     6061 tggcgaatat tttaatcatg gactgggtca tggtataggt cttggagatg gtgaagtccc
     6121 cactttaaat ccaaatagtg aagatatatt agtggaagga atggtaatgt cttgtgagcc
     6181 aggtatttat attccaaatg tagggggagt tagaatagaa gatgacattg ttataataga
     6241 tggaaaaggt gttccactca ataagacatc caaagagttt ataatattag gagagtaaaa
     6301 ttatgaaata taattttaat aaagtattaa atcgtaaaga aggaaattgt agaaaatgga
     6361 gcaaccatgt gattaaagaa aaattcggct taaatgaaga tgcaattcct atggatttag
     6421 ctgatatcga ttttgagtgt gctcctgcta taaaagaatc tatcttaaaa agagcatcag
     6481 taggagatta tagttatact tttataggag atgacttcta tgatgcgatt ataaattgga
     6541 ataaaagaag atttgatgta tatatagaaa aggattggat aaaattaact tttggtactg
     6601 tttcaaccct tcattatata gtccaagcat atactaaaga aggagagggt gtattgataa
     6661 atacacctgc ttatgaccct tttgcagagg ctgtagtaaa taataataga aagttgtgtt
     6721 gtagctcatt aaaattagat aacaatagat attatttaga tttagaggat atggaaaatc
     6781 aaatgaaata cgagaatata agattgctta tattctgtag tccacaaaat ccatcaggtc
     6841 gtatatggac aaaagaagaa ctatatcaag tgtcagagtt atgtttaaag tataatgtaa
     6901 ttttggtaag tgatgaaatt catagagata taatatttaa agattataga tttgtatctt
     6961 tatggaatgc tcatccagaa atttataaaa atagtataat ctgtgtatct cctaataaag
     7021 gcttcaactt aggtggatta aaaacatcat atgtattaat aagagatgta aaaattaggg
     7081 agactttatt agaaagacta aaaagtaatt caataacttc tccaaatgta tttgcaatac
     7141 cagcaatagt tgcagcatat aatgaaagcg aagaatggct agatgctatg actagttatg
     7201 tagaggagaa ttttgaaata gtttatgatt tctttgaaac taatatacca aaagctaaag
     7261 tgatgaagtc agactcttct tttttagcct ggatagatgt aagagaagta tttagaaatg
     7321 aagaagaatc taaagaattt tttagacatg caaacttaac tatggtagtt ggaagttatt
     7381 ttgtgaaaga tggagatgga tttattcgta ttaatgtggg gtgtcctaga gaaacattaa
     7441 atgaggctct aaatagaatc aaaaaaacgt atataagtat gtattgttaa atcataaaaa
     7501 aagtatctat ggtttatata aaaacacatt atttagttat caaatattac tggaattttg
     7561 tattccagta atattttttg attaaggaat attattgagg aatattaaat aatattattt
     7621 cataccctta tattaactta tataaaaata ttttattatt ttgggaacgg gttagctaac
     7681 ctttatctag tgtatattta tattgtaaga agcagtaaaa acttttcctt tactaagttt
     7741 attaataaac ttcttatctt tataaatttt gttagaattt atactatcag caagaccata
     7801 ccaaggttct atacataaaa aaggagcagt agaattagtt tcactataat aaggagtcca
     7861 aataccaact agagggaaat cttccataga tatagttaca tatttactac catcattatt
     7921 acatatagat acttcatcta tgttggtgta tatcaaagca tcattcttga atacttctgg
     7981 atttaactgt aagtttttta attttccaat aggtattata tcatctgtaa aagaaccatt
     8041 taaattgatt ttttctacat catttttact tttaaactca aggtagtgtt tagaaaaatc
     8101 attttgttcg taaaaaggaa ggttgaatgc aggatgtcca cctatagaaa agaatatatc
     8161 tttagaatca gtatttttta caatccattc tacctcaata ctattatcat ttaatgtata
     8221 gtttatgatt agctcaaaag aatatggata cttctttaaa gtagattcat tatctgttaa
     8281 tttataagtt acaaatgtat ctcctttatc tacaatctca aagtccatat ctctagcaaa
     8341 cccatgttga gtcatgctat ataaattttc ttctattatt gtttcattat caaaaagttt
     8401 acctactata gggaacaata taggtgattg tctaccccaa tattttttat taccatccca
     8461 aagaatgttt ttgtcgtatt ttttagaaaa tattttagtt agttcagcac caaaactgct
     8521 tgattcgata attaaatttt cattttgtaa tatattcatt taaaatctcc tttattatat
     8581 tttatgagta tattatacta taaagtataa agattattaa attttatttg atattatatt
     8641 tgaaaggtag attttagtat attttgaaaa ttttgttttg aaaatttggg attatttaag
     8701 aaattttgta ttataaatag aaggaaatag ataaattgta tagaataaaa tatattaaga
     8761 taagttaata atgttaaatg ggaagtgagt ttatggaaaa accaaataca aaagttgtta
     8821 actttcaaga gtataaagac agaaaaaacc aaagagaagt aaatagagac gaacttttaa
     8881 agcttataaa aaaaattgtg acttcaaaat agggagttgt taaactccct attttaatgg
     8941 gtttcataat aattaagaca ttctgagtcc atctatataa tctttgaatt gtgagaaatc
     9001 tgattcacta cttaattcta tggtttctga accttccgta taaaaattct taagatagat
     9061 agtataatta ttatcaacac aatcatgata aatacatcta tatccatctt catacaaact
     9121 ttttaactta gaaaaatcat ccacaatatc atctccttaa agttctttta aatatatttt
     9181 tacaaaaagc atctaattgt atgcaaattg acaatgtaaa ataaaggaaa tgaacataaa
     9241 atgtagaata atttttatat gtatgtaaaa ataaaaagaa aaagttgaaa tttttagaaa
     9301 ataagagtat tttttatcta aaaagagagt ttaaaaaata aaaaaatgtt gggaggataa
     9361 aaaatgagtt tattagaaag tatatcaaaa aacatatatt ccctagataa ctcttctatt
     9421 gaaaaaacaa agcagagatt ggataggctt atacatccaa ctggaagctt aggaaagata
     9481 gaagatattt gcatgcaatt agcaggaata tttggcaatg aaaattttga cacaagtaaa
     9541 aaggttataa ttgcttttgc gggagaccat ggtgtatatg aagaaggcgt agcacctgac
     9601 cctcaaaata taacaaaatt acagtttcca aatttttcga aaggtctatg tggagttgga
     9661 gtaataagta aatttgtagg ggcagatgtt gtagctgtag atgtaggaat aaactgtgat
     9721 gaaaaactgg atggggtatt agactacaag ataagaaaag ggacatcaaa tatggcaaaa
     9781 ggtccagcta tgagtaaaca agaagcaatt agatgtttag agataggtat tgaaatagca
     9841 gagcagtgta tagaaaaaga ttataaggtt attggtattg gggagatggg tatagcaaat
     9901 actactccaa gtacagcaat aatatctgta atttcagggt gtgacccgtt agaagttact
     9961 ggtataggtg caggtcttaa gaaagaaaga ctaaagcata aggctgaagt aattagaaag
    10021 gctattgaaa ttaataatcc aaatccaact gatggagtag atattttatc taaagtagga
    10081 gggtttgaaa taggctcaat ggcaggtgtt atattaggtt gtagtgctaa taggattcca
    10141 gtagtaattg atgggtttat ttcttatgca gcagcattaa ttgcatataa gataaatcca
    10201 aaaactagag aatatatgat agcatctcat ttatcagcag aatcaggaac taaaagagca
    10261 ttagacatat taaaattaga ccctctatta aatatggata tgagattagg tgagggaagt
    10321 ggagctgctt tagcatttga tataatagaa gcttcaaact atacttataa aaatatggca
    10381 acttttgatg aaattgacat gggaagataa atataaaaat ttaatatgta ggagtgtttt
    10441 gatgagtaaa attattctag taacaggagg agctaggtca gggaagagta actttgcaga
    10501 aaaactatgt tttgacagaa ataataatac agcttatata gcaacctcta ttccatttga
    10561 tgatgaaatg aaagatagag taaaaaaaca tcaagagaat agacctagaa actggaatac
    10621 ttatgaaata tatgaagata tatattccat aattaaggag atatataaag aacatgaaac
    10681 tgttatacta gattgtgtaa cattattagt aaataatcta atgtttgaac acaatctaaa
    10741 tatagaagaa tctagtcaag aagaaataaa caaattagaa caatacataa aagaacaagt
    10801 tgtcaaactt attgaagaaa tagaaaaaac aaatctatac tttgtatttg taacgaatga
    10861 gttaggtatg ggaattgttc ctgggaataa acttagtaga atttatgctg atattgtagg
    10921 tcgtataaat caatatatag cttcgaaaag taatgaagtt tattttgtag ttagtggcat
    10981 agctatgaag ataaaggagt agtatgcaat tatgaaaaga tttattttaa tactacagtt
    11041 tttgacaagg atacctataa aattaaatgt aggatttgat gatgaatttt ataagtcaat
    11101 agtatatttt ccacttgtag gttttgtcat aggtatatta tcctatttaa taggatggat
    11161 ttctatgctt ttatttgaac catttatagc ttctataata ataactctag ctggtgtctt
    11221 aattactgga ggacttcata tagatggatt aggagataca tttgatgcaa tatatagtta
    11281 tagggataaa gaaaagatgc ttgaaataat gaaagattca agattaggta caaattcact
    11341 tctagcaatt atgtttgtac ttttattaaa ggttggattc gtttacgata taataagtaa
    11401 taattcattg tgggttataa tttttatgcc aatgattgca aggttaggag taatgttatt
    11461 gacatataaa actgtgacac caagagaaaa aggaatggga aatttattta taggaaaatt
    11521 aactacaagt atgttaataa ctgctataat atacacatta ttgatagttg ctcttattac
    11581 taaatttata tttttattac ctaatatagt gttgattaaa gtattaggtt cgattatagt
    11641 ggtttttgta tttataatat tattcaaaaa acatatttat aaaaaaattg acggagtaac
    11701 aggagatata ttaggctgtg gaatagaact ttcagagctt gtatatttaa tttacatata
    11761 tttgttaatt tttatgtttt tttaaagtaa aaagtggtga taatatgata agattaatat
    11821 taataagaca tgcactgaca aatgataata aaaaaggaag attatcaggc catataaata
    11881 gttgtattag tgaagagggc aaactacaaa taaataaaat taccagatat ctgagtaatg
    11941 aaaacataga taaggtttat acaacaccat ctacacgtac taaagataca gtagaaaaaa
    12001 tatcaaaatt gaaattatta gaaatagaag aaaaagaagc tttaagagaa attagttttg
    12061 gagactttga aggaagaact tttgaagaaa ttaaaatcaa atatccaaat gagtttgaaa
    12121 aaatgataaa agaaggtaac aactatagat atccaaatgg agaaagtttg atagactctt
    12181 ataaaagagt ggcaaaagaa attgataata taattttaga aaatgatagt aatttagata
    12241 caaagacaat acttatatgt tctcatgcag gaacaataag aaatataata acacatttga
    12301 tttctggtag ttataagtat cactggaact ttaaaattga taatgcatct attacagttt
    12361 tagaaattga tggtggattt gctgtaatag ataaaatgaa ttttacagac ttcatataat
    12421 ttaatcaaaa ccaaacatta caatctgtgt ttgtaatata tctgtaaaat tagcaatgaa
    12481 aatagaatat tagattaaaa ttaggttctt aattagatgt taaaagggaa aaaggttaga
    12541 tgcctttgca gcccccgcta ctgtgaaacc aacgaaacta taaataccac tgtcagtttg
    12601 atgggaaggt tatagaagta ggatgaagtt aagccaggat acctgcctaa tttaatttaa
    12661 aacaagaggt cttcggggtg aaagattttt aattaatata tataaaagtc ctatctttag
    12721 aagtattagg acttttttta atttgttttt tagtaaattt taacaaggag ggatggaaag
    12781 tggggaaaaa aataatgcta caaggaacag cttcaaatgt aggtaaaagt gtcttagtag
    12841 caggtttgtg tagaatattt aagcaagatg gttatactgt tgctcctttt aagtcacaaa
    12901 atatggcttt aaattctttt ataactaaag aaggattaga aatgggaaga gcacaagtat
    12961 ttcaagcaga ggctgcgaaa atagagccaa tagctgatat gaatccagtt ttattaaaac
    13021 cttctggaaa taataaaagt caagttattg taagaggtaa agttgtagga gaaatgcctt
    13081 cttctaagta tcatgattat aaattggagt tagtagatgt gttgaaggaa acatttaata
    13141 gcttaaattc aaggtatgat attgtagtta tggaaggggc aggaagtaat gcagagataa
    13201 acttaaaaga tagagatatc tctaatatgg gtatggcaga aatagctgat gcacctgtaa
    13261 taatagtcgg agatattgat agaggaggcg tatttgcctc attagcagga acaatgcttt
    13321 tattaaatga agatgagaaa agaagagtaa aaggtgtaat aatcaataaa ttcagaggaa
    13381 aaaaagagct tttacaagat ggtgtaaaga tgctagaaga catcataaaa gtacctgtta
    13441 taggagtagt accttataca gatataaaga tagaagatga agacagtgta actacgagat
    13501 ttaagaaaaa aatgaataaa agtgatatac acatagaaat aattagaact ccacatatgt
    13561 caaattttac agattttaat atatttgaga cacaagaaga tgtaagtatt agatatgtag
    13621 actatggaga atctttagga aatccagata tattaataat acctggaact aaaagtacta
    13681 tagacgattt aacatatata agaaaaagtg gtcttgaatc gcaaatcaaa aatttacata
    13741 gtcaaggtaa attgatattt gggatatgtg gtggatacca aatgttaggt aaaaaattaa
    13801 aagaccctta tcatgttgaa agtgaaattg aagaagttga tggaataggt cttttagata
    13861 cagaaacaac gtttgagaaa gaaaagacta caacacaagt agaagctact attgttgaat
    13921 gtattagtga atacatgaat aaccttaagg gaaaaaaagt aaaaggttat gaaattcata
    13981 tgggaattac aaagcgaggt aatgatgtaa gaagtttaga tttaataact aaaaaacttg
    14041 ataaagaagt gaattatact gagggaagtg taaataaaga ggggaatgta attgggactt
    14101 atcttcatgg aatatttgat gaaataagct ttacaagagc aattttaaac aatataagaa
    14161 agaaaaaagg tttaggagaa ttagaaagca aagtaaactc ttttaatgaa tttaaggata
    14221 aagagtatga taagttggct gattttttaa gagaacattt agatatagag aaaatatatg
    14281 agattatgaa ataatgatga cctttattta taatttaggt aggaggtgta ttatgaaaaa
    14341 aattttaatt gcgggtacta atagtggagt aggtaaaaca acaatatcat taggaataat
    14401 gcaagctttg gttaagagaa atatgaaagt acagccatat aaagtagggc cagactatat
    14461 agacccttca tatcacactt ttataactgg aagacattct agaaatttag attcttatat
    14521 gttagatgat gaaaaaataa agtatatagt aaataagtcc tcaaaagatg cagatatttc
    14581 agtaatagaa ggtgttatgg gcttatatga tggctttgga atagatttag ataattgtac
    14641 aagttcacat acttcaaagg tattaaagtc acctgttata ttagttataa atggtaaatc
    14701 tatggcagca tctagtgctg ctatggtact tggatataag gaactagata aagatgtaaa
    14761 tataaagggt gttatagtaa ataatgtaaa gactaatagc cattatcaaa taattaagag
    14821 ttctatagaa aaatattgta atgttgaagt attggggtat tttcctccaa ataacaaatt
    14881 ctctttggaa tctcgacatc taggattagt tcctagtgta gaaatgaaat cactaaggga
    14941 aaaattttat accttagcag atgaaattga acactatata gatattgaca gaataattga
    15001 aatatcagaa agcgatgaat ttgagagtga ttttgatttt caaactttaa tagaaaataa
    15061 taaaataaaa gaacatgtta ataataaatc aatagctata gcatatgata aagcattcaa
    15121 tttttattat agagaaaata tagagctttt agaggaactt ggtttagaaa taaactattt
    15181 tagccctctt gaagatgtat cagtgccaaa tgcagattat atttatattg gaggaggttt
    15241 tccagaaata tttggaaaag aattagaagc aaatgagtca atgagaactt ccatattaga
    15301 agcacataac aataatatac ctatttatgc agaatgtgga ggactgatgt acctaggtga
    15361 gaaactattg aatcaagaag aacaagaatt taatatggtt ggaatattta gtggaattag
    15421 taagatgaca tcttctttaa aaagatttgg gtactgtatt ggtgtagcca aggaaaatac
    15481 tcttttatct aaaaaagggg aatctattaa agggcatgag tttcatcatt caatatttga
    15541 aagtgatgaa aaatgtgtat attcaatgag aaaagaaaga gatggaaata tagtagaaga
    15601 gtgggatggt ggttatagta aaggaaacac tttggctaca tatcttcata ctcattttta
    15661 taataactta aattgtatag aaaatttttt gaaaaaagga aatgaataaa tgagtatttt
    15721 atctatttat ataggatatg tgacagactt aattgtagga gacccatatt cttttcctca
    15781 tccagtgaga tttattggta aacttataaa ttttactcaa ggtataataa gaaaggtctc
    15841 taaaaatgat aggcagttaa aattgggggg atttatatta tggtttataa ccgttggaat
    15901 aacatattta acaacttatg ctatagtgaa gttatttagt tttaatatat tattgagtat
    15961 tgtagtgaat agttttataa tttatacaac actagccaca aaatgtttaa aagacgaagc
    16021 attaaaaatt tataatgtat taaaaactga agatatagaa aaatctagga ttcagctatc
    16081 ttatatagta ggtagggata ctactaattt aagtgaacct gaaataataa gagcaactgt
    16141 agaaactgtt gcagaaaata ctgtagatgg tataattgcg cctatgtttt atgcatttat
    16201 tggaggagca ccacttgcta tggcctataa agctatcaat acattggact caacagttgg
    16261 atataaaaat aaaaagtata aagatattgg ttttgcatct gctaagattg atgatatagc
    16321 caattatata ccagctagaa tatctgtcat tcttatgaca attggaagtt tctttttaaa
    16381 ttataactat aggaactgtt ttaaaatctc tattagagat agaaagaatc ataaaagtcc
    16441 aaattgtgct ttttcagaag gtgcagtatc tggtgctcta ggaatacaac taggaggtac
    16501 caacgtttat tttggggaaa aagtatataa gccaacaata ggggataagc ttagggagat
    16561 agataaagaa gatataatta aaactaataa aattatgtat gcatcttcat ttgtatctat
    16621 gttggtattt acactaatat atgtgttatg taggatgatt ttaattaact taattatata
    16681 aattattgtt tgttaactta agggggacat agatgaagga tttagggcat ggtgccaatg
    16741 tagatgaaat ggcaagatta tatgggaaaa acccaaaaga aataattgat tttagttcaa
    16801 acataaatcc aaatgtatta ccaaatttag agagatacat tttaaaagga ttagaagagt
    16861 gtaggaatta tccagatata aattatacaa acttgagaga aaatatatca aaatatatag
    16921 atataaatcc agactttata atacctggca atggtgcaac agaagtgatt tatttgctta
    16981 tgaagagtat taaaaaaaaa ttggctataa taaatccgac tttttcagag tatagaagaa
    17041 gtgctgagtt aaataattta gatattatag atttagagtt agatttagaa aataacttta
    17101 aacttaatat agatataata aaagaaaata taaaaagatt tgatagttta tttatatgta
    17161 atcctaataa tccgagtggt aatgtccaag atttaaagga acttgttcat ttactagata
    17221 aacataataa ggtattaata attgatgaaa ctttcatgga atttgttgaa gatgaaagta
    17281 aatatagttt ggttaaatat atagaatcaa ataaaaatat atttataatt aaagctgtga
    17341 ctaagttttt tggtatgcct ggtttaaggc taggatatgg tcttacaagc aacactgaaa
    17401 ttatgaacaa aatttatgaa cacaaagagc catggactat caattctttt gcagatatac
    17461 tatccaattt tatttttgaa gataaagagt atattaaaaa tagtaaagaa tattacatag
    17521 aagaaagaga atatatgtta caagagttga gaaacataag aaatataaag gtctatgata
    17581 cagatgctaa ttttatcttg ataaaaatat ataaaaaaac aactaaagaa ttgaaaaaag
    17641 atttgtttaa gcaaggaaac atattagtta gagatgcgtc taattttata ggattagatg
    17701 atagttttat aagagtggct ataaaatctc atgaagataa caaaattctt atagaaaata
    17761 tgaaaaattt attgggtgat taaaatgaaa tcttatggaa tatgcccagc atcttgtgga
    17821 gaatttgtac aaggtataat tgatgatgaa gaatatttat gttcatatgc aatagatatg
    17881 tattctaaag tgtatattga agaaaaatta gtagatataa atttagggag atataagtca
    17941 aggttagcaa ttgaaaaggt atttgaaaaa tttaatttac ctaaaaaata tactaaaaat
    18001 atttctttaa atataaatag taaaatacct gttggaaaag gaatggcaag ttcaactgct
    18061 gatataggag ctactataaa ggcaacatta tcattgatag ataaagattt aagtagcgag
    18121 gagatttcta aattagcagc agaaatagaa ccaacagatt ctatatttat agataaaaat
    18181 agtatattta atcctctaaa tggaactgtt ataaaatacc taggaaatct gaccaatgcg
    18241 aaagttgtaa tacttgaacc aaacaaagta ctagatacta tgaaaattag attgaggcaa
    18301 gattataata aattaaaagt agagaataaa gaagtaataa aaaaatcatt tgctctttta
    18361 gaagaaggat tgaaaaaaaa taatttatct ctagttggag aggcttgtac tttgagtagc
    18421 ttagcaaatg agaatataga aaaaaaagag tatttaaatg aaataataaa aatatcaaaa
    18481 aaatatggag catatggagt aaatattgct cacagtggaa ctgtagtagg tattttaatt
    18541 gataagttta tgaatgataa aaagatgata gatgccttat gtgaatctaa cataaattct
    18601 gtctataata aaatttatac acaaaacata attaatggag gaattaaggg ggaaatagaa
    18661 tggaatacat aaagaatcct atgaaaatag aagaaaaaag ttttgaattg atacaagaaa
    18721 taatagacga aattagacca gattataaat ttaagaataa tatcgaagaa aaaataataa
    18781 aaagagctat acatactact gctgattttg attacttaga tatattaaaa atatcagaag
    18841 aagcagttga tagtataata gatgcattaa aaaataatgc aagtatttat actgatacaa
    18901 atatggctct tagtggtata aataaaagaa agttagaatc attaggatgt aagtataaat
    18961 gtttagtggc agatgatgaa actgtaaaat tagctaaaga aaaaggaatt actcgttcta
    19021 tggcagcagt agaaattgca gctaaagaag aaggaagaaa aatattcgtt ttaggtaatg
    19081 ctccaacagc tttatataaa gttatggaga tgaaatcaga aggtagatta gatttagatg
    19141 cagttatagg agttcctgta ggttttgtag gagcacaaga atcaaaagat gaagttgaaa
    19201 aaacagatat accatatata atttctaaag gtagaaaagg tggcagtaat ttagcagcag
    19261 caatagtaaa tgctatatta tatactatgt aggtacctat atggaagaat atgtatatat
    19321 agatggaaaa aaatatagaa gaggatatac tacaggttca tgtgcaacag gtgcatctaa
    19381 ggctgctgta tatatgttga ttacaaaaaa tagaattaat actataaata tagatactcc
    19441 taaggggata ccattactac taaaagttga taatataaat atttctgata catttgtgga
    19501 atgttccata aaaaaagatg gtggagatga tatagatgca actcatacca tggatattta
    19561 tgcaagagct gaaattgtag ctaaaaatga taagaacaaa gggtatctta ctctaaagga
    19621 tatagatagt ttaagcacca atagtgagtg taaaagtgag ctctataagt ttattagggt
    19681 ttatggcgga actggtattg gtgtggttac gaaaaaaggg cttagtgtgg atgttggtaa
    19741 gcctgctata aatccgactc cacttaaaat gataaatcat gaaataagaa agcttatagg
    19801 agataatttt gaatctatac ttggaaatga taaagtttta aaaataacaa tctttgctcc
    19861 acaaggagaa actgtagcaa aaaaaacatt taatccaaga ttaggtatag ttggaggaat
    19921 atctataata ggtacaactg ggattgttga acccatgagt gatgatggtt ggaagaagtc
    19981 tctatctata gaacttcaga tgaaaaaaga acaaggctta gataaaataa tattggttcc
    20041 aggaaatcat ggagaacagt ttataagaga aaaattaaac ttagatatta agtatgtagt
    20101 tagggtaagt aattttatag gctatatgat taaagaagct caaagaatag gatataaaaa
    20161 aatcttaatg gcaggccata taggtaaatt tataaaagta tctgctggta tttttaatac
    20221 tcatagtaag gtagcagatg ctagaagtga gatattagtg gcaaacttag ctttaatggg
    20281 agctagatac gaatttttaa ataaaataaa tcaatgcgta actactgaag aagctgtaga
    20341 gctaataaat aatagtgaat atagagaagt atataatata ttaagtaata aatgtaggga
    20401 gagagtaaag caatacttga atgaagattc agatgatatt gatgtggaag taatcatatt
    20461 ttcaatggat aagtctttat taggtaaatc ggataataca gatgatttag tggaggtctt
    20521 tatatgataa acataatagg tctaggacct ggtaacttag actatataac aaaaaaaggt
    20581 gaaaatttaa tttccacttc tgatgtatta attggtggta aaagaaattt agagtctata
    20641 aaaaattttg aaggagaaaa aatagttctg gattctaatt taagagaaat tatagagtac
    20701 ataaataaca ataaagaaaa gcaaatatca ataatagcat ctggggaccc attaatatat
    20761 gggataggta gatatctatc taaaaatatt gataataaga tgttaaacat ggtttctggg
    20821 attagttcat tacagtacat attttcaaaa atatatgtag atatgaatga tgtatatatt
    20881 acaagtagtc atggcaaagt accagatttt gattacatat tatcacataa aaaagtatgt
    20941 atggttacag attcaaagat aggaccaaaa caaataagta gagaaataat agatagaaat
    21001 ctaaataaaa ttattgttgt aggagaaaat ctgtcatatg acaatgaaaa aataacaata
    21061 gcaaaaccag aagagataat aagaatggac aattttgata tgaatgttgt ggtgatttta
    21121 gatgaggaat agtgaattta taacaggtaa agttccaatt actaaggaag aggttagagc
    21181 aatatcaata agtaagttaa atttagtaaa tgctaaaaac ctcatagaca taggggctgg
    21241 tacaggaagt gtaagtgttg aagctgcata taattatcct aatttaaagg ttatatctat
    21301 agagaaaaat gatgatgcta tagagcttat agagaaaaat aaaaaaaaat tcaacttaaa
    21361 aaatatagaa gtgataaaag gatatgctcc aattaatcta gggttaaatg ttaaagttga
    21421 gtctattttt cttgggggaa caggaaataa tctggaagaa ataatagaat ggtctaaaaa
    21481 aaatctcgtt acaggaggaa gacttgtagc taattttata ataatagata catttaatca
    21541 aactttaaaa ctgcttaaaa agcatggatt taaagaaata gatgtatgtg ttttgaatgt
    21601 ttctaaacta gagaaactag gaaaaggtga atattttaaa ccactaaatc ctatatatat
    21661 aatatcttgt gaaaaagggg aaaatgatga tgaataaagt acattttgta ggagcaggac
    21721 ctggagataa agagctgata acattaaaag gatacaaatt attaagtaat gcagatgtag
    21781 tgatatatgc aggttcactt gttaatcctg aacttttgga atactgtaaa gaagattgcc
    21841 aaatacataa tagtgcacat atggatttac aagagataat agatgttatg agagaaggta
    21901 tagaaaataa taaatctgtt gtaagactgc aaacaggtga tttttctata tatggttcaa
    21961 ttagagaaca agtggaagat ttaaataaat tgaatataga ttatgactgt actccaggag
    22021 taagttcttt tttaggggca gcatcatctt taggggttga atatacagtt ccagaaattt
    22081 ctcaaagtgt aattataaca agaatggaag gcagaactcc ggttccagag aaagaatcaa
    22141 tacaatcata tgctaagcat cagacatcta tggtaatatt tttatctgtt caagaaattg
    22201 agaaagttgt gtctaaatta ctggaaggtg gatatccaaa agatactcct atagcagtta
    22261 tatacaaagc aacttgggca gatgaaaaaa tagttaaagg aactttaagt gatatagctg
    22321 taaaagttaa agaaaacaac ataaataaga ctgctttaat aatggttggg agatttttag
    22381 gagaagagta taataattca aagttatatg ataaggattt caaacatgaa tatagagggt
    22441 aatattgcta taatctgtat tacagaaaat ggaaaaaaat tagctctaaa gataaattct
    22501 ctagtaaata actgtgttat atatcatgta aaaaataaaa aaagtaattt agttgttgaa
    22561 gaaacatgtg tgcatacagt tcaaaagaag ttaagtgatt ttgtaggaga aatatttagc
    22621 aaatttgatt acatagtatt tataatggct acaggtatag ttgtacgaag tatagcacca
    22681 tacataataa gtaagtttag tgaccctgca attttggtaa tggatgaaaa aggcaaaaac
    22741 gtaataagtt tattgagtgg tcatattgga ggggcaaaca gcttgacaca gtacataagt
    22801 agtcttattg atgctaatcc agtaataaca acagctactg atataaatca aaaagcttcc
    22861 ttagatatga tagcaaaaaa actaaatgca catattgata attttagaga aagtgtaaaa
    22921 gaagtaaatt ctatgctagt gaataataaa tgtgttggat tatatattga tgaaggatat
    22981 caagttgata ctaggggatt taaacttata gaaagtaagg atgagttaag agatagtttt
    23041 tcaaacacat atatagaaaa attagttgta gtaagcaata aaataaattg tattgaagat
    23101 attgagaatt taaacctaga taataaattt gaacattgct atagtaccaa aaaaataata
    23161 aaggtaattc caagagatat agtgatagga attggttgta gaagaaacac agatagtgaa
    23221 ctgatgtata attcacttga agagtttctt aataaacaaa atatagatat tagagcaata
    23281 aacacagttg gaagtataga actgaaaaaa gacgaaaaag caataattga attatcaaaa
    23341 atattgaagg tgccatttaa aacattttca gtagatgaaa tatctaaggt tgataatctt
    23401 tttgataaat ctgattttgt taaaaaaaat gtgggcgtgt attcagtagc agaacctgta
    23461 gcatatcttt tgagcaaagg taatttgtta gttgaaaaac ataaatataa aggaataact
    23521 ttttcagtgg ggagaataaa aatatgatat acgttgtagg aattggacca ggaagtaaag
    23581 atacaatgac tttagaagca ataaaagcaa tagaagattc tgaggctatt gttggatata
    23641 agacttatat aaaactaata gaagaattta tacaagataa agaaataatc caaaatggta
    23701 tgaggcaaga agttgataga tgtaaacaag cagtagagga agcaaaaaaa ggaaaaaaag
    23761 tagcagttgt aagcagtggt gatgctggaa tatatgggat ggcaggtctt attttagaat
    23821 taatatctaa agaagatgaa gatataaaag taaaggtaat tccaggtgtt actgcaagta
    23881 ttggtgcagc agctatactt ggagctccta ttatgcatga tttctgtcat ataagtttga
    23941 gtgatttaat gactccgtgg gaagtaattg aaaaaagatt gagattggcc gctgaagccg
    24001 attttgtaat ttgtttgtat aatccaagaa gtaagggaag aagtgaacat ctaagcaaag
    24061 catttaaaat aatgggtgag tttaaaggtg gagatacacc tgttggagtg gtaaaagatg
    24121 taggtagaga aaaagaagaa aagtttgtat gtacttttga aaatatggac tttgaaaaag
    24181 tcgatatgac tacaatggtt ataataggaa ataagtctac ctatattaat ggtgaattga
    24241 tgataactcc aagaggttat acagtatgat tttaatttta ggaggaactt cagatagttt
    24301 atctatatgt gataagataa atgaactgaa aaatcaacca tatatccttt cagttactac
    24361 agattatgga agggaacttg cacaacaaca tgcagagaat gtaattttag gtaagcttgg
    24421 aaaagaagat atgctaaaat ttataaaaga taataatata gtaaaaataa tagatgccac
    24481 tcatccttat gcagttgaag tatccaaaac tgctattact tgtgcaaaac tattgaatat
    24541 agattatata aggtttgaga gaaaatcttt aattgaagaa atagattatg ataataaatt
    24601 tatagtagat acaattgaag aagcctgtga aatagcaaat aaaataggga caaatatatt
    24661 cataggaaca ggaagtaaaa atttgaacag atttatagag gaaataagtg ataaaaattt
    24721 agttgttaga gttcttccaa cgagtgaagt catattaagt tgtgaaaaat taggtttaag
    24781 tgcagataat atagtagcta tgaaaggtcc atttagccag tatataaatg aagaaacata
    24841 taaacattac aatatagatt tagtcataac aaaagaaagt ggaatagctg gtgggttctt
    24901 agagaaggta aatgcatgta aaactttcaa tataccagta ataattataa aaagagaatc
    24961 gattaactac gaaaatgtat ttaatgacat aaataaaatt aaagacatac ttgtgtaatt
    25021 tttaggagga gaatatatga agaaagcctt attagtagtg agttttggta caagctatca
    25081 tgaaacgaga gaaaagacaa tagatatatg tgaagataaa ataaaaaatt cgttaaaaag
    25141 ccatgatttt tttagagctt atacatcaaa tatgataata aataaaataa aaaaaaggga
    25201 tggaatagaa atagataatc caatacaggc tttagataaa atatatgaac aaggatatga
    25261 tgaagtaata atccaaacac ttcatattat atgtggcgaa gaatttaaca aattaaaaga
    25321 acaagtagat ggatattctt ctaaattcaa aaagattgta ttaggaagac ctttgcttac
    25381 acatattgaa gattatcagg aagctgtaga ggcaattaag catcaaattc ctcatatgga
    25441 aagtaatgaa gcagtagtat ttatgggaca tggaacactt catgaatcac attcagcgta
    25501 tccagcattg gaatacatgc ttagggataa tggaataaat gcttatgttg gtacagttga
    25561 agggtatcca gaaatagaac atgtaataag aagattaaaa gaaggaaata taaaaactgt
    25621 aaatttgatg ccatttatgt tagttgcagg agaccatgct ataaatgata tggcaggaga
    25681 tgaagaagat tcttggaaaa caatattaga agaaaatgga tttaacgtaa gagttcattt
    25741 aaaaggacta ggagaaaatt cttatataca agataaattt gttcgtcatg ctgtaaaatg
    25801 tgcagagaat gcgaagttct atggtatagg agcagggcca ggagatggtt ctttacttac
    25861 gataaaagcg gttaatactt taaagaaact ggatattcta tacactccag aatcaaaaaa
    25921 gggtggagat agtctagctt tatcaatagt tagtgaatat ttacctgaat cagtggaaat
    25981 aaagtctagg catttcccaa tgagttttga tgggaatgaa aaaagtttag cttgggacaa
    26041 tgtggcacaa gaaattatat cagatgttaa tgatggtaaa aatgtaggat ttgttacatt
    26101 aggagaccct atgatttata gtacatatgt atatataatg aaaagaatat taggagaggt
    26161 agaagtcgaa actataccag gaatttcatc attttccaat atagcatcta atcagaattt
    26221 tccattagtc atggataaag aagctttagt ggtaattcct tgtactatgg aagatgataa
    26281 gatagagtat gctttgcaaa attataattc aatagtattg atgaaagtat ataaaaattt
    26341 taaagaaata ataaaaaaat tagaaaagta tgatttaata gaacatgcta ttttagtaag
    26401 taattcttcc caagaaagcg aaaatgtgtt tacagattta aaagaagcgc accttgaaga
    26461 taagatatct tatttttcta ctatactagt aaataaagat aataaaataa aataggtgaa
    26521 agcaatattt aaaggagact taatatgaat atagtggttg gaactagggg tagtaatcta
    26581 gcgcttatac aaacagaatg ggtaataaat gaattaaaaa agaagtatcc agaaattagt
    26641 tttgagatta aaatcataaa gacaaaagga gatttaattc aaaatgtttc actggataaa
    26701 ataggtgata aaggattgtt tgtcaaagaa atagagcaac aattattaga tggaaaaata
    26761 gacatagcag tacatagtat gaaagatatg ccttcttatt tagcagatgg actaaaattt
    26821 gctcatacgc ctaagagaga agacccaaga gatgttctaa tacttagaga aggatataaa
    26881 aatttagatg atttacctca tggagcagta ataggaacag gaagtaaaag aaggaagttt
    26941 caattattga aacaaagacc tgatttaaat atagtacaag ttagaggtaa tgtggaaact
    27001 aggatacgaa aaataaaaga tgaaaacatg catggtattg tgttggcagc atcaggaata
    27061 attagagcta atttgcaaga taaaataagt tcttatttac ctgttgatgt tgttattcca
    27121 gcacctgccc aaggagcttt agctatagaa attagaagca atgatagtgc gatagaggga
    27181 atagtaaatt cactgaagga tgagaataca gaaatccaaa tattggctga aagaggtttt
    27241 ttagatggtg taaatggaag ttgtcatatt ccaatggcag cttattgtga aataatccaa
    27301 gataaaatac atcttactgg cttatatgga gattcagaag gaaaaaaagt agtaatcaag
    27361 tctatagatg gagatatttc ttctcctaga gagttaggtt taaagttagc taaacttgta
    27421 ttgaaggagt atgagaatta tgaagggtaa ggtttactta gtaggggcag gaccaggtga
    27481 ttataaactc atgactttaa aaggtttaga gtgtataaga aaatcggatg taatagtata
    27541 tgatagatta gcaaatagta gttatttaag agaagctaag cctgattgtg aacttatata
    27601 tgtaggcaag gcatcaagta atcatatttt gccacaagaa gatataaata gaattatagc
    27661 agaaaaagct aaagaaggaa aaattgtaac tcgtcttaaa ggaggagacc cttatgtatt
    27721 tggcagaggg ggagaagaag cagagacttt agtagatgaa ggtatagaat ttgaagtagt
    27781 tccaggtata acttctgcta taggaggttt atgttatgcg ggtattccta taacacatag
    27841 agaccatgct tcgtcatttc atgtaataac aggtcatttg aagggtgatg atagtggaga
    27901 actaaactgg aatgctcttg caaataacaa aggtactctt gtgtttttga tgggaatttc
    27961 aaacctagag aaaataagtg aaaacttaat gaaagaagga aaagataagg atacacctgt
    28021 tgctcttata agttgggcaa ctagatataa tcaaagagtt gttacatcta cacttgaaaa
    28081 tgtttatgaa actgctatta aagaagaagt aaaaccgcca actcttatag tagttgggag
    28141 tgttgtagga cttagagaaa agttgaactt ctttgaaagt aagcctttat ttggaaaaag
    28201 tattgctgta actaggtcta gaaatcaaaa tagtgtattg gtagaaaaaa taatggattt
    28261 aggtggaaat cctatagaaa tacctactat taaggtagaa aaaatacaaa ataatattaa
    28321 cttagaaaat gaaataaaaa atataaataa gtataattat ctaatactaa ctagtaaaaa
    28381 tgccgtagaa atcttctttg aaaagatata tgaaatgaat tttgatttaa ggatattatc
    28441 taatttaaag gtatgtgcta taggttctgc tacatcaaat gagttgaaaa aaagaggaat
    28501 tatagcggat atagtaccaa aaaaatttgt agcagagtca ctgtatgaag agttagcgcc
    28561 tattttaaat gatgacgata atatacttat acctagagca gaaaatgcta gagattatct
    28621 ggttgacaaa cttaaggcta tttgtgaagt aacagaagta catatatata gaacagtaat
    28681 agatgaggag aaaaaaggtg agatattaga tattttaaat tctaatgaca tagattatat
    28741 aacatttact agttcctcaa cagtaaataa tcttgttgaa attataggaa aagaaaatat
    28801 taaaatatta gaaaaaataa aaacactatc tataggtcct ataactacag agacaataaa
    28861 tagtttggga ataaaattgt acaaagaatc tgctacatct actatagatt cgttagtaga
    28921 tttaattata aaaaattaat tttggaggct tgaatatgtt aagaagacca agaagactaa
    28981 gagcaagtaa agcaatcaga aatttggtaa gagaaactaa ggtgaatgta gaagacttaa
    29041 tatatccact gtttgtagta gagggggaaa atataaaaga agaaattaaa tctttaccag
    29101 atgtctatca tttatcaata gatatgctag aagacgaaat aaaagaaata agtgagttgg
    29161 gaattgaaca tataatgtta tttggtgttc cagatgaaaa agatgagtgt gcttcacaag
    29221 cttttaatga taatggaata gttcaaaagg caattagaaa ggtaaaagaa ataaattcag
    29281 atataaatgt aataacagat gtctgtatgt gccaatatac aagtcatgga cattgtggga
    29341 ttttaactga tactggttat gttgataatg ataaatcatt ggagtatttg gctaaaatat
    29401 ctgtaagcca tgctaaagca ggagctgata tggttgctcc atctgatatg atggatggaa
    29461 gaatacaagc aataagagaa gctttagatg aaaatggatt agaaaatgtt ggaatcatgg
    29521 cttatagtgt taagtatgct tcaaattatt atggaccttt tagagaggca gctcattcag
    29581 caccatcttt tggagataga aaaacatatc aaatggaccc agcaaatagc aatgaagcta
    29641 taatagagac tgaacttgat atattggaag gtgcagatat acttatggtt aaacctgcat
    29701 tatcatattt agatattata agaagagtta aagataattt tgacttacct ctagcagctt
    29761 ataatgtaag tggagaatac tctatgctta agtttgctgt gaaggaaggt ttattaaatg
    29821 aagatgtaat tcttgaatca attacatcta taaaaagagc aggagcagat ataataataa
    29881 cttattttgc taaagacatt gctaaactgt tgaaaaaata aattctagca gtataaagaa
    29941 tatcaaaaag tataggaatt gtaattaact attatacagt agttttgttt agacatttat
    30001 tgaagaataa tagaaactaa tttgcaattt ctatacttta tttatgtaac taaataaaat
    30061 ttggaggtag aagatgctat atcctattaa tttaaaatta gatgagttag atgtagttat
    30121 aattggtggt ggagaagttg catataggaa atgtaaaaat tttttagagt ttaacaaaaa
    30181 tgttactata gtttctaaac aaattttaaa taaattttat gatttaaaag gaaatataaa
    30241 aataataaaa gatgattata aagaaatata tataaaagat gcttctgtaa taattgctgc
    30301 cacaaataat agagagttaa atatggaaat aggattatat tgtaaagcaa aaaataagct
    30361 agtgaatgta gttgataata tagaaatatc gaactttact gttccatcat atataaaaag
    30421 aggagattta cttataagtg tgtctactgg gggcaagagt ccttcattat catcaaagat
    30481 aaaaaaagaa attgaagaga gatatactga agattatgag gagtatttaa atgtattagg
    30541 agatataaga aaaaaggtta taaaaaaata cgaagataaa agtaaaagaa aagatgtgct
    30601 taatatgttg gtaacacttg acctagaaga acttaagaag tttgatatat aaaaaataaa
    30661 ataaaaaatg ttaaaaaggt gttgacggat tatttttaag
//
LOCUS       DAMJWS010000045        29391 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMJWS010000045 DAMJWS010000000
VERSION     DAMJWS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.045"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            92..1804
                     /gene="ptsP"
                     /locus_tag="PC399_002142"
     CDS             92..1804
                     /gene="ptsP"
                     /locus_tag="PC399_002142"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF2592999.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            2127..3677
                     /locus_tag="PC399_002143"
     CDS             2127..3677
                     /locus_tag="PC399_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2593000.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            3703..6405
                     /locus_tag="PC399_002144"
     CDS             3703..6405
                     /locus_tag="PC399_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2593001.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSAQQIVHKQCANDILSCIENCGLDKKYISVEVTEKVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQITKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(6911..7576)
                     /locus_tag="PC399_002145"
     CDS             complement(6911..7576)
                     /locus_tag="PC399_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593002.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            7920..9350
                     /locus_tag="PC399_002146"
     CDS             7920..9350
                     /locus_tag="PC399_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2593003.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            9718..10296
                     /locus_tag="PC399_002147"
     CDS             9718..10296
                     /locus_tag="PC399_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2593004.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            10326..10472
                     /locus_tag="PC399_002148"
     CDS             10326..10472
                     /locus_tag="PC399_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2593005.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            10777..12156
                     /gene="scfB"
                     /locus_tag="PC399_002149"
     CDS             10777..12156
                     /gene="scfB"
                     /locus_tag="PC399_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF2593006.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            12185..12682
                     /locus_tag="PC399_002150"
     CDS             12185..12682
                     /locus_tag="PC399_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF2593007.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            12980..14644
                     /locus_tag="PC399_002151"
     CDS             12980..14644
                     /locus_tag="PC399_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF2593008.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            14657..17107
                     /gene="recJ"
                     /locus_tag="PC399_002152"
     CDS             14657..17107
                     /gene="recJ"
                     /locus_tag="PC399_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2593009.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDDSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            17120..17632
                     /locus_tag="PC399_002153"
     CDS             17120..17632
                     /locus_tag="PC399_002153"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF2593010.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            17706..19913
                     /locus_tag="PC399_002154"
     CDS             17706..19913
                     /locus_tag="PC399_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF2593011.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            19938..20387
                     /locus_tag="PC399_002155"
     CDS             19938..20387
                     /locus_tag="PC399_002155"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF2593012.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            20402..21016
                     /locus_tag="PC399_002156"
     CDS             20402..21016
                     /locus_tag="PC399_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2593013.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            21107..22663
                     /locus_tag="PC399_002157"
     CDS             21107..22663
                     /locus_tag="PC399_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF2593014.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            22653..23915
                     /locus_tag="PC399_002158"
     CDS             22653..23915
                     /locus_tag="PC399_002158"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF2593015.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            23989..25776
                     /gene="aspS"
                     /locus_tag="PC399_002159"
     CDS             23989..25776
                     /gene="aspS"
                     /locus_tag="PC399_002159"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF2593016.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMLTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            26235..27467
                     /locus_tag="PC399_002160"
     CDS             26235..27467
                     /locus_tag="PC399_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2593017.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            27481..28287
                     /locus_tag="PC399_002161"
     CDS             27481..28287
                     /locus_tag="PC399_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF2593018.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(28399..29268)
                     /locus_tag="PC399_002162"
     CDS             complement(28399..29268)
                     /locus_tag="PC399_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2593019.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
BASE COUNT        11648 a         2978 c         5282 g         9483 t
ORIGIN      
        1 attataagta aataaaacta taaaaatgta aattatttat ataaatttat aaagtttatg
       61 tgctagatga tattttttag gaggtagagt tatggcttac aaaggaatag gtgcttctcc
      121 tggtgttgct ttagggaaag cgttagtagt tgaacatagt gaattagtaa ttgaaaaaaa
      181 atctatagat aatgttgaag ctgaaattgc taaattagaa gatgcagttg cagtttctaa
      241 agaagaatta gtaaaagtta aggaaaaggc ttctgaagaa ttaggagcag aggaagcaga
      301 aatatttgaa gcacatttat tagttttaga agacccagag ttaattggtt ctgcaataga
      361 taaaattaaa actgaaagtg taaatgcaga gtatgcttta aatgaaataa aagagatgtt
      421 tgttagcatg tttgagtcta tggacaatga gtacatgaaa gaaagagcgg cagatataaa
      481 agatgttaca aatagaatac ttagacatat attaggaatt aaagttgttg atttatctgc
      541 tttaagcgaa gaagttgtat taatagcaca tgatttaact ccatcagaca cagcaactat
      601 gaataaaaaa atggttttag gatttttaac tgatataggt ggaagaactt ctcatactgc
      661 tatcatgtct agaactttag aaatagctgc aatcgttgga ttaaatgata taacttcaaa
      721 agttaaggat ggagattttg tggttttcaa tggagacaca ggtgaagtaa tcgtgaatcc
      781 tgatgaagag actataaata aatatactga attaaaagct aagtatgaag atgagagaaa
      841 agcattacag ctattaaaag gaaaaccatc tgtaacttta gacggaaaac atgtggaatt
      901 agctggtaat ataggaacac ctaacgatat agaaggtcta ataaagaatg atgctgaagg
      961 tgttggtctt tatagaacag aatttttata catggataga gattctttcc caacagaaga
     1021 aatacaatat gaagcataca aagctgtatt agaaggaatg gatggaaagc ctatagttat
     1081 aagaacattg gatataggtg gagataaaga attaagttat ttaagtatgg aacctgaaat
     1141 gaatccattc ttaggttaca gagcaataag actttgccta gatagaaaag atatatttaa
     1201 gactcaatta agagctttat atagagctag tgttcatgga agattaagaa tcatgttccc
     1261 aatgatttca tctttagaag agttgttaca agctaaagaa gttgtaaaag aagttttagc
     1321 agaacttgat tctgaaggtg tggcttatgc taaagatgta gaaattggta tgatgataga
     1381 agttccatct gctgctgtaa tatctgatgt attggctaaa cacgtagatt tcttctctat
     1441 aggaactaat gatttaattc aatacacttg tgcagttgat agaatgaatc aaaagataag
     1501 ctatctttac aatcaattta atccagctgt tttaagactt ataaagactg ttatagacaa
     1561 tgcacataaa gaaggcaaat gggcaggtat gtgtggagaa tctgctggag accaaaaaat
     1621 gataccaata cttttaggta tgggattaga cgagttctct atgtctccaa tatcaatact
     1681 tcctgctaga aagttaataa cttcagttaa agagtcagat atgaaaaaat tagctgatga
     1741 tgtacttaat atgggtactg ctgaagaaat aaaaagctat atagaaaaga cttttaatat
     1801 ataattaatg ttatatagtt actaaaattc atttttctaa ataacaaaat tatattttac
     1861 gaataaaaaa actatagtat atcatatata ctatagtttt tttgttatat aaattacgta
     1921 tcaaaatttg taaaatgaat agatataatt actaaatatg tgatagtatt ggctataata
     1981 ggatataaaa aataatattg taaaaaaaag tttatattta gtaatgaaaa tttaacgttt
     2041 tacttgaaat tatgtgtttt gtaatgtatt attaaggctc taataggaac attttcatca
     2101 atttttctat agtgggggta ggaattatga ggtacaaaaa aagaaatata gataagtcaa
     2161 gtgaactttc atttcaatct ctagcaaaat attttataac tttctatata gtaattatat
     2221 taattattct ttctatgttt tgtataaata caatatgggt tatgaaaaaa gataatcaag
     2281 atttattatc tacaacatca aatcgtattg aagatagggt aggagaaaca attaagctat
     2341 tgaactcttt gagtaaagat aattacataa tagataatag tgtgaatggc atagataaaa
     2401 gtaaagaact ttcaatgtat agtgatgaat ttgggtatat gatgatatgt tttgttgata
     2461 agaatataat tgtgtgggat gaagaaggtg gtaatattag tctagctggt cgtgattata
     2521 tgcagaaact ttttgcaaca aaaaaatatc aagttacaga tagttttgca gcaggggctg
     2581 atggaaaaac tttaaactac actattgcat cacctataat aaagaatgga aatgtagatg
     2641 gttgtgtatt tgcagcaatt tactttgatg agataaagga tatactcaaa caaggaaaag
     2701 gtcaacagca acagaatttc atattatttg gaagtgaaaa tcaaataatg aatttaacaa
     2761 gtgatgaaac tgcatatgga gagaagtata tgaagttatc gcagaaaaca aaatatcttg
     2821 gaaccaatgc aaaaaaagta gaaaaggata tgtttaataa taaatcaggt gattattggg
     2881 cttttgaaaa tggaaaatta tactttatgt cctataaacc aattaagaac acaaattgga
     2941 gtttattttg taaagttaat ttcttagata tttttgcatt aaatttaatt cctatgggag
     3001 ctattctatc tgttataact ttagtattta ttattttgat ttgtatggag aaaagcttca
     3061 tgaaaaaacg aatggaagtt attgatatgc ttttaggttc tgttcaagaa ttagaaaaac
     3121 gtatatatca aacagaaaga cctagtgaag tagactttaa agaaattatt cagctcacaa
     3181 gtaaaggtct gactgatgga ttaactggtg ttgtaacacg tactgtattt tttgaccaaa
     3241 tttataatcg tgttgaaatg gaggaaaatg ataaattatc tgtaatttca tttgttgatt
     3301 tagatgattt gaaatgtatt aacgacttat atggacatga aattggggat atggttttga
     3361 aaaatgttgg atatgtactt cgtgaatatg agataaaata taatggttta attgggcgct
     3421 atggtggaga tgaatttgtt atgttgttat ctggttttac atcgagagaa gaggtggagg
     3481 atttattgga tgagattgct attcgcttac atactgaagt actaacagat gatggtatca
     3541 ttcaaactca ttgtagtgtt ggagctgtag tatgtagtgt aaaagacaat aaagatatag
     3601 ataatttatt aaaacaggct gatgaagctt tatactatgt taaacgtaat ggaaaaggta
     3661 attatcattt agtttaatgt atgtggaagg agtttatatc atatgaaaaa tgtatctaaa
     3721 aaattttttg tatgtactat tattgtatta ataacatttt tatgttttag taaatttgct
     3781 atttatgttt ttgacaaaca agaaaagaaa gaaacaaaag tagtagaaaa catgttaaat
     3841 gtctacaaga ataacattat tttaaatctg actgaaaaat ttaattatag caaagacttg
     3901 acatcagttt tagtttcaaa caattattct atagataaat taaaacctat tgcagatgaa
     3961 attcttaaaa acaattcttg catatatatt tcagttgtag aaaaatataa tgtagctgca
     4021 tttttttcta attctgtcaa aaaagatatt atcaatactc caatacagga actgaattat
     4081 tcttattcat tagcaaattc tatcaaagga ttagtcgtag aaggtccttc taaagaccag
     4141 ttttcaaatg gagatgtgtt tttaattact aatcctattt ataaaaatgg agagtactgg
     4201 ggagaaatta cagtagcttt agatgccaaa aaagttgtag attcttttaa cttaaataaa
     4261 ttaaaagaat ctggatttga gtatgaactt tggcgtgtac acctagataa tggttcaaaa
     4321 tctacaatag aaatttctac taaagatttt aatttttcca aatcacatga aattaagttt
     4381 aaacttccga ctgtttggac tcttagcatt gtgtacaaaa atggatggaa taataagaat
     4441 atagatttta ttgtatttgt tatattgtta attatttcta tattaatagg tgtgatgaca
     4501 tttatttaca ttgaaaagaa gtcgattagt aaggaattaa atcaacagag tcttaaaaat
     4561 ttagaaactg gtattttgaa tagagaaggt tttttaatta agttgcaaga gcatctgaat
     4621 agaaaaaata atgaaatagt gttgctgtat atatgtatta ctaattatag taaatttgct
     4681 ccagttatga gctttgaaaa aagacaagat tatcttacac atatagtaaa aaatctagat
     4741 aattacataa aaagttctca ttttattgct cgtataagtg aggaaggttt tgttctagct
     4801 ttagaagaaa attgtgataa aaatgaattg cttaacattg aaaaagggat ttccttagag
     4861 cttttacgaa aaattaaagt agaaaataaa caagtatttt taacgattgg ttatggctca
     4921 tctaggtgtg ttgatgatag aaaagatgca gaatctctgt tactttcagc tattgaagat
     4981 tatgagagaa gatttccagc aatgtctcct attcatgatt ttactaaaaa gtgtgaagat
     5041 atgcttgatg aaaagaaaca gattgtattt ggtgaatatc cagatttaga aataaacaag
     5101 ttgtctaaag taatatacaa atattataaa aaagtacagc gtattatgta taaagattat
     5161 aatattgata ttggaaatag gcgagggtat atgcgtgatg tcgaagtatt aattgattat
     5221 aattctgaga gaatgttata cttattttta atagacatat gcaagtttgg aaaatttaat
     5281 gaactgtttt ctattgaaat aggagatgtt attttaaaag aagttagtaa caaattaaaa
     5341 acaatttttt ctaattatct atatcgtatt aatggagatg tatttttagg catttcaata
     5401 gataaagaat cttacttaga tactttacat aaggttaatg aagtatttaa tagttcttta
     5461 agtgtagaag atgtactttt tgatgtaaaa tgtaatattg gtatttgtca atatccatat
     5521 catggtgaaa atccatatga ccttctggaa aaggtacaaa ttgcattgga gtatgcaaaa
     5581 aaggatgtca aaagtaatgt taaagtttat gataaggaat tgacagaaga gatttaccat
     5641 gaaaataata ttattaagga tttaaaaaaa ggtctggaag aacaaacact agaagtttgg
     5701 tatcagccaa tttttaatgt tagtaaaaac tgttatataa aagctgaggc actaattaga
     5761 ttgagaaata aagagggcgg ttattattct gcctatgaag ttattactta tgcagaaaag
     5821 catggactta ttaaggatgt aggtctatat gtactaaaaa aggcttgtga tgtaactaca
     5881 aaaataaaag ataaaaatat agactttgaa caaatgcaat taaatttatc tgctcagcag
     5941 attgtacata agcagtgtgc aaatgacata ttatcatgta ttgagaattg tggtctagat
     6001 aaaaagtata tttctgttga agttacagaa aaagtactta tggaaacttt tgaaattgta
     6061 aatgttactc ttgataaact aaaaaaagaa ggcattagca tagctttaga tgagtttggt
     6121 tctggattta gtagtataaa ttatctggca aatttaccta ttgaaaattt aaaaatagat
     6181 aaggaattag tagaacaaat tacctattct aaaaaacaat ttgaattatt aaaaactata
     6241 gttcaaatta ctaagattaa tggcatggat attgtagcag aaggtgttga aacaaaagaa
     6301 actctggatt tagttgtaac ttcaggtgtg aatttgattc aaggtcatta tttttcagaa
     6361 cctttaagtg aagataaatt tattgaactt atgattgaag aataaaggtt aataaaataa
     6421 agatttaagg tattgaaaaa taattaaaag tatttaataa tagaattagg gaagaataag
     6481 tttataagta aaaacaattg attagtagtt aataaaataa atgtgatatc caaataaatc
     6541 ctaaatctaa tttgagtttt aatatagata gtctaaagtt tgattattaa agatttaatt
     6601 gtaagttgtt acagttgatg atattgatta gagattaaag tttagagtag aaagttacct
     6661 taaatttaaa aatgcaagat aacccaatct aaaaattata atcactcgat aggtttaagg
     6721 atttatttgt ttattataaa taggagggtg tctcaaaatg aactttttag tttatgaggc
     6781 actctctttt atatagaatt aaatatattt tattatttca acaatgaaaa agaagcttat
     6841 ctctattttg agacagcctc tttgtatact attattaatt taatcaataa cataatttaa
     6901 aattataata ctagttaaga tttcctgctt tattagtaaa taattcttta atagcattgt
     6961 ttctggaaac taaatcgtta tcagagttgt ttgttttttt cataatcact gaataacgtt
     7021 ttttatttat aaaaaatatt aaaatcttat catcatcaaa attgtattct ttatatctgt
     7081 cgatagactc ataacctaga tttagaatgt ttttgtcttt tatactatct acatctaaaa
     7141 tatatgatgg gtcagccttt agtaatatat taattaaaca tttttcttca ccagcacaga
     7201 ataactccat cttacttgat atagatttag attgctctga agttattttt gcaggaataa
     7261 ggttgtcatt atcatctctt agaagataac catgtgtgtc ttgttgatat actaaattaa
     7321 cctttttctt aggaacacta atcattttat ctaaatgtac ataatgatga ttttcaacaa
     7381 aaccttcatt taatatttca acaaagttat taattttacc taggcaaaaa gattctatag
     7441 attctttaca gaatttattt gtaaatggta ttatgtttac gttttcctcg tctgtattcc
     7501 atgctatagc ataatccaac gaatatactt cttcctctat atctgcaaaa ttatattgta
     7561 ctatgtttcc tttcaaaatt actcgacctc catcttataa tatatttaat atactttata
     7621 aaacttatat tatatttaaa taatatcata aattgaataa atatgatata gtaaataaga
     7681 aaaaatttga aaaaaatcac tataaatgag taataaatat aaatagtgta tactaatggg
     7741 atggaaaatg tccactatta actttattac cccacatata taagataaaa ttattaatat
     7801 aaaatataat ctatcgacaa tgaatagact tttaaataaa cttaatataa aattaattaa
     7861 ataagtgctt aaaaacgatt tactttaatg atagaatcat attaaaggag gataatttta
     7921 tggatataaa gaataaaatt aaatctatat ttttaccaga aaaaaatgac tatttagatg
     7981 atgaagaatt tgagtatgat atagaaaata atgaagaaga tttaaaggaa gataacaaag
     8041 tacatttaaa tgatttaagc agagtaaaat atgattacag agagttaaaa gacatagaaa
     8101 accaaacaga agagatgtgt ctgctagcag taaagcagga tggtactata attgagtttg
     8161 taaaggacaa gacttataaa gtatgtatgg aagcagtaaa acagacatat aaatctctta
     8221 agtatataac aaatcaaaat gaggatattt gtatagaagc agtaaaacag aactacagag
     8281 ctttgtacta cataaataat aagacagaaa atgtactaat tgaggctata aaaaatgcat
     8341 caacttatga tgtcatggaa gtatttaaat ttgtggaaga gcaaacagaa gatgtatgtc
     8401 tagcatttat agaaagggca tctaaaaatg atgttgcaga gatattaaaa ggaataaaag
     8461 agcaaactcc agccatatgt ttagaagctg taaaaaaaga tggaaaatct ttagcttatg
     8521 taaaggaaca aagtaatagt atatgtttag aagctgtaaa agaaaactac agtgcactat
     8581 cttgtgtaaa agagcaaact gaagaaatat gtatagaagc agtaaaacag aatgactttg
     8641 cattgtatta tgtaaatgaa cagacagaaa aaatatgtat ggaagcagta aaaagaagtt
     8701 atatggctct acagtatgta aataagcaaa cagaagagat ttgtttggaa gcagtaagaa
     8761 ttgatggaag agcattacag tacgtaaaag agcaaacaga agaaatttgc ttggagtcag
     8821 ttagacaaaa tggaaaagta ctgcaatatg taaaaaaaca gactgaaaat atatgtatag
     8881 aagcagtaag aggttcattt gaagaacttg aaataaaaga aatattaagt tatgtaaaga
     8941 taccaacaga aagaatattt gtagaagcag taaagcaaaa tggaaaaatt cttaaatatg
     9001 tagaaaatca gacagaatta atatgtttag aggcagtaag agaaaactat aatgctttag
     9061 cttatgtaaa ggaacaaaca gaaaagattt gtttagaagc agttaatcaa agttatgaag
     9121 cgcttaaata tgtgaaagaa caaacagaag aagtttgtct aaaagcagta aaacaagatt
     9181 atagaatgtt gaaatatgta aataatcaaa cagaaaaaat atgtttagaa gcagtaaagc
     9241 aaaattatag agctttagaa tttgttgata atcaaacaga aaaagtttgt cttgaagcag
     9301 taaaacagaa tagaaaagca ctacagtatg taaaacaaaa acaaagttaa aaggctaaaa
     9361 tatatagaaa acatatgcaa ataaataaaa tttgatattt tgtaataaat aaaatgggta
     9421 tctcaaaagg aactttttag tttgtgaggt actcttttta tatgaaatga aatatatttt
     9481 tagcctttta acaatgggga aaaggtttat ttctattttg acataggctt tttatgcata
     9541 taaagcttga taaaattgtt ttattataat tgttttgtta tataaataat aagaagaaaa
     9601 ttatataggt ctttttttga ccagttttac atttttaaca actagttttg taaagtatat
     9661 tgaattttct caaaaaagaa tgtactctat atatgtaaaa tttttttgga ggtaaaaatg
     9721 tttaaaagat atgctgaaaa aatgacatct gttttaattt gtaataatat gatagataat
     9781 aatgaatcta aagtctattc ttatggattt gaaatattaa tagctttcat agttaatata
     9841 accaccatgc tgtttatagg ttttttattt ggtaaattta catatgtatt atttttttta
     9901 atgtgttatt gtccaataag acaattttca ggagggtatc atgcagacaa ttattttaga
     9961 tgcttattaa cctttatttt tataatttta tctacaatct taattataga aaatataaat
    10021 atagacttat ttaaaaatat tattatgatt attgcaagtg ttagttgggt tggaatttgt
    10081 gttctgtgcc caatagaaca taggagtaac cctataagtg atagggaaaa attggtttat
    10141 aagaaaactg ctatatttat atctacagta gtgttattga taacaatttt aagtctttct
    10201 ttgagtatat ttgtagatta tttcacatat agcgcatttg ccatgttttg gatttttgtg
    10261 atgttagtat taggtaagtt aaaagctaaa gtataagtaa aatatgtatg agggagagtt
    10321 aagttatgaa aaagtttatt gtaagattta tgaagtttgc tagctcattg gcacttagca
    10381 ctgctatttt gtcagctaat agtacctgtc cttggattat tcatcagcct aaagtaccca
    10441 aagaaatcag caatctaaag aagacaaatt aaatacaagc acttatttat acaaaattat
    10501 caggaaaaaa gaaattatta tattttaata agattaaaac aatataaaat cagatacaaa
    10561 atttatttta tttattttat aggagcctaa tacctatttg gtaatatatt agtgcgccaa
    10621 tttaggggga tatagtgtat ttaatgaaat gacttacgga gagtaagtca tttttgtatt
    10681 gtatgtattt tatgtaaact tatgatataa tgaagggtaa agtttaatta aaataataaa
    10741 aaactttagt atagatagta atttaggagg tagggtatga gtatgataca taaattttct
    10801 atgaatggat ataatatcgt tttagatgtg aatggtggag ctgttcatgt gttagatgat
    10861 gttgcatacg atttgttaga tttttataaa gaaaaaagta aagaagaaat attagagata
    10921 ttaaaatcaa aatatcaaga agaaaaaata aatgaagcat atgaagaaat attaaatttg
    10981 gaaaaagagg gtcttttata tacagaagat acatatcaat atcatccaag ctttgtacat
    11041 agagagcctg tagtaaaagc tttatgttta aatgtagctc atgattgtaa cttaaaatgt
    11101 aaatactgtt ttgcagctca aggtgatttt ggaggagaaa aagaacttat gagttttgaa
    11161 gttggtaagg cagcaattga ctatttgatt gctaattcag gaagtagaaa gaacctagag
    11221 atagatttct ttggtggaga gcctttgatg aactttgagg ttgtaaaaca actggttgac
    11281 tatggaagaa gtgtagaaaa agattataat aaaaatataa gatttacaat aactacaaat
    11341 ggtgtactgc taaatgatga gattatagat tatataaatg aaaatatgca taatgtagtt
    11401 ttaagtttag atggaagaaa agaagttaat gataatatga gaccaacttt aaatgataaa
    11461 ggaagttatg atattacatt accacgattt aagaaacttg tggaaaaaag agcaaaagat
    11521 aagtattatt atataagagg tacttttaca agagataatt tagatttttc aaaagatgtt
    11581 atgcattttg cagatttagg atttaaatta acctctgtag agccagttgt tggagatgag
    11641 agtaatccat atgcacttag agaagaagat ttacctaaga tatttgaaga gtacgaaaaa
    11701 tttgctgtag agtatgcaga tagacaatta caaggagatg gatttaaatt tttccatttt
    11761 atgatagacc taaatcaagg tccatgtgta ataaagagaa taactggatg tggagctgga
    11821 aatgagtatc tttctgtaac tccaaatgga gatatatatc catgtcatca atttgttgga
    11881 aatgaagaat ttaaaatggc aaatatattt gatgaagaaa tagtacttcc agaaaattta
    11941 aaaaatatgt ttagagaagc tcatgtatat actaaagaag agtgtaaaca atgttggaat
    12001 aagttttact gttcaggtgg atgtcatgca aatgcgataa actttaataa tgacatatca
    12061 aaaccatatg aattaggatg tgaaatgcaa agaaaacgta cagagtgctc tataatgata
    12121 caagctaagt taatgttaga aggagcaacc aactaaattt atattttaaa ggagttgttt
    12181 atatatgatt agagattatt tagaagataa acctttaatt gacgaaagtg tatttgtggc
    12241 aaaatcagca gatgtaattg gaaatgttaa aataggcaaa gattcaagta tatggtacaa
    12301 tgcagtagtt aggggtgatg aaggaccaat aactattgga gaaaacacta atatacaaga
    12361 ttgttctata gtgcatggag atacagaaac tataattggt aacaatgtaa cagttgggca
    12421 taggtctata gttcatggtt gtaaaattag tgataatgtg ttaattggta tgggttcaat
    12481 aatattggat aatgctgaaa taggagaata tactctgata ggagcaggta cactaattac
    12541 atctaataag aagtttccac caggtgtttt gataatgggt tctcctggta aggttgttag
    12601 agagcttaca gaagaagata aaaaatatat tgatgaatct tatgaatggt atttagaggc
    12661 agcacaaaat caaaaatatt agtttaaaga gtggttgttt caaaacatat ttaaatctgt
    12721 tttgaggcaa ccactaattt attttattag attttgttga tattttagtt ttcaaaaagt
    12781 atttgattaa accagatata aaatgtcaaa aataaaaatt aactgagtta aataatttta
    12841 ataaaattgg tagtatatgt gataaaatta aatattaaag aggatatagg atattagaat
    12901 atcaattggt gaaataatgt catatacgat aaattaaggt gtaagttttt gggtatacct
    12961 atcttaggag tgaaaagatt tgagaataag ttatagaaat ttaaagcgat atagagaaat
    13021 tggatatgtt ttaataaagt atggttttag ttttattgta gaaagactaa atatagaagg
    13081 aattgcctat aaaataccac tatttgaccc accagaagaa ataaaaaata tgacaactgg
    13141 tgagagaatg aaaagagttt tagaagaact aggacctaca tatataaaaa taggacagat
    13201 attgagtaca agaaaagact tgttagacca ggatataatt gatgaaatat caaaactcag
    13261 agatgatgta gagaaatttg attcaaatat agctattgat atatttaaag aagaagtggg
    13321 tttaagtata gaagaaatat tcttagaatt taaagaagaa ccaatagcag cagcttcaat
    13381 tggtcaagtt tatgaaggtg tacttaaaac gggagaagaa gtaatcgtaa aaattcaaag
    13441 accaaatata gagaaaataa taaaatctga tttagagatt cttagaacta ttgcaaatac
    13501 tttaaaagat ttaaaaaagg attttaattt agatttagta cagatgattg aagaatttca
    13561 aactcaatta atgagggaac ttgattatac atttgaagct ataaatgcaa ctaaattctc
    13621 taggatattt aaaaatagtg atgaagtata tatacctaaa gtatattcag aatataatac
    13681 taagaaaatt ttagtaatgg agaaagttaa tggtacaaag ttaagtgatg tagaaaaaat
    13741 aaggagacta ggttataaca caaaaactat tgttgaaata ggagttaggt cattttttac
    13801 acaggtatta tctcatggat tttttcatgc agacccacat ccaggtaata tattcgtagt
    13861 tgctaaaaat aagatagcat atatagattt tggaatgatt ggaattattg ataataaaac
    13921 tttaaaccaa ttaaatgaaa ttgcattagc aggtgtagaa aagaatgttg acaagataat
    13981 atatctattg atagaaatgg atgcattaaa tggagaggct gatattaagg gacttagaca
    14041 ggatttactt tatctaatac attattacta tgatatttct atagaaaaaa taaatgtaac
    14101 agatatatta aatgaactat ttaggttttt tagacagtat aaaatagtca tgcctgcaca
    14161 atttgtaact cttgcaaaga cagttataac attagaagga acaagtagaa ctttaaacac
    14221 agatttttct tttggttcta tgggaaaaga gtttatgaaa catcattata agagtaaatt
    14281 taaccctaaa aatgttgttt taagttcaag acaaaatgta gaagaaatat tactggacat
    14341 aaaaactatt ccaaagcaat taaaagcaat tttaaaaaat atagaaagaa ataatataaa
    14401 aatgcaaatt gaagatgtta aaatgacgag gttagaaaat tgtataatag agctaacatc
    14461 tcaaatatct ttgagtttag tgttggcatc tattatagta ggttcttctc ttataattgc
    14521 atctccaaat atagaaaata atatatggat aaaatttact gcaatagcgg gtttctttat
    14581 atcctttata ataggccttt gcttagttat aagaagtatt aggtcaaaat acaaaaaaga
    14641 ttagggggaa ttaaatttga aaaaatggat attgaagtat aaaggtaaga tagaggaaag
    14701 tgaatttagc aaaaaactta atatttcccc ggaaatctgc caaattttaa aaaatagagg
    14761 gatatgtact gaaaaagatt cagaaatatt tatgaatcca tctttagatt atttaagaga
    14821 ccctttttta atgaaagata tgcaaaaagc tgttgataga ataaagttag cagttgagaa
    14881 gaacgaaaaa atttggattt acggagatta tgatgttgat ggtgtatcat ctacatctat
    14941 cttgtgctta tattttaaga gtataggtta caatgttgat tactatattc ctaatagatt
    15001 agaagaaggg tatggtataa atgaagatgc tattaagctt ataagctcta ggggatgtga
    15061 cttgattata agtgttgact gtggaattac atcagtgtct gaggtcaata cagcgaatga
    15121 tttgggcata gatgttataa ttacagacca tcatgaatgt caaagtgaaa ttccatcagc
    15181 ctttgcagta ataaatccaa aacaagaaga ttgtaattat ccatttgatt cactatgtgg
    15241 atgtggtgta gcatttaaaa tgattcaagc cttgactcca aaagaagaat ttaaaactag
    15301 tatgtataat tatttggaaa tagtaacact agcaactatt tgtgatattg ttccacttat
    15361 agatgaaaat agaataattg taaaaaatgg tttaaaatca atgaaagaag gcaaaaatat
    15421 tggattaagg gagcttataa aagtttgtgg tgtagaaagt gataagatag gttcttctca
    15481 tataggtttt gcaataggtc ctaggataaa tgcttcaggt cgattgggat attcttactt
    15541 aggagtagag ctatttacta ctcaatcaca agaagaggca gtagaaatag ctagtatatt
    15601 ggaagaaaaa aataatgaaa gacagatgat agaagctaag atgtatcatg aagctgaaga
    15661 aatgctaaag tctaatagca gatacaatga tgacaaggtg ttggttttag ctaaagaggg
    15721 ttggcaacat ggaataatag gaattgtagc atcaaagtta actgaaaaat actacaaacc
    15781 aacaattctt ctaggtatag aaaatgggga ggctactggt tcagcgaggt caataaaagg
    15841 atttaatata tttgaagctc tcataaagtg caaagacttg atgactaagt ttggtggtca
    15901 tgaacaagca gcagggttat ccttggattc agacaatgta gaaattttag caaatgaaat
    15961 aaataaattt gcagattata atttaactga agatgatatg atagagaatg taaatgttga
    16021 gtttgaatta caagaaaatg taataaattt aaatcttgta gaagaattac ataagttaga
    16081 accctttgga ttaaataatc ctaatcctag atttatagtt agaaattata ttttaaaaga
    16141 tttgaaagtt ataggtaaga atcaacaaca tctgaaatta agcatagaaa aagagaaaag
    16201 ctatgaatgt ataggattta atatgagtca tttaaaatca atgtataaag taggagacaa
    16261 ggtagatgta ctattccaat tagatgaaaa taactatatg ggaaatagga aagtacaatt
    16321 tttacttaaa gatatacgat tggctcgacc taaaagtgca agtaatgaca aactttcatt
    16381 gaaattaatg tcaaaaataa taccaaaaga tactcaaagc ctgtataata tatctgtgtc
    16441 tgattttgaa ttatttgatg gaaatacaga tataaatata tttgattatt ttgaaaaaga
    16501 tacactaata atatctaatt ctataaatgg attttataga gcaatgtcag atatatcact
    16561 tatagatttg gattttaaca taaattacaa tattattgaa gatgatagca aaaatactga
    16621 taaattagag cttatatttt ctcctaatat tgataaaata gacttaaaaa gatataataa
    16681 tattattctt tatgattatt tatacaataa aggcgaatat tcgtatatat atgagaataa
    16741 aagagaagaa agtgaaatta taaaatatta taataaaaca gacctattat atttaaagaa
    16801 tgtagttagt aatatagttc catcaagaga tgaatttata acaatttata aacaagcact
    16861 cataaaaaaa gaaattgatt tggatatggt aaatataagg gaaactttta atgtaattcc
    16921 attaaagttt tttacgatat taaatgtatt tagagaatta aatttacttg actttaattt
    16981 aaattatgaa aaaaattctg tactcataag aatattgcca aaaccacaga aaaaattaga
    17041 cttaaatgag agtttaatac tcaataatct aaaaaattta gaaaaacaat acaatagcag
    17101 ttattaggag gcgtttccca tggatttaaa aaactttata aggaatattg atgatttccc
    17161 aaagccaggc atagatttta aagatgtgac aactctattt aaagatggag atgcatttaa
    17221 atatgcagtg gattctatag ttgaagaatt gaaagataaa gatgtagatt tagtaatagg
    17281 accagaagca agaggatttt tgatgggtac acctgtagca tatgctttag gagtaggatt
    17341 tgttccaatt agaaaacctg gaaagttacc aggtgaagtt gagagttatg agtatggatt
    17401 agagtatggt acagatactt tagagataca taaggatgca attaaaaagg gtcaaaaagt
    17461 agcaatagta gatgatttat tggctactgg tggaactatg gaagccgctg ccaagcttgt
    17521 agaaaaatta ggtggagaag tagtttctat gcaatttttg attgagctaa aattcttaaa
    17581 tgggagagaa aagttgtcta actatgatgt taattctctt ataaaatact agtactttta
    17641 taatagttgg tataacacca actattttca tatatattaa aattttatac aaaataggtg
    17701 gttatatgca tgataaagaa ttacaagaaa taattgaaaa aataaaagtt tatgcaccta
    17761 atgcaaattt tgaaatggtt gaaaaagcct ataatcttgc aaaaaaagcg catgaaggac
    17821 aatatagaaa atctggggaa ccatacttta ttcatccatt agcagttgca aatattttaa
    17881 tagagatgga attagatatt gaaactataa cagcaggttt acttcatgat gttgtagaag
    17941 atacagaata tacctatgaa gacataaaaa aagaatttgg caaagaagta gcggatttgg
    18001 ttgatggagt aactaaactt ggtcaaataa aatatcgttc gaaagaagaa actcaatctg
    18061 aaaatttaag aaaaatgttt ttggcaatgg cgaaggatat aagggttata cttataaaac
    18121 ttgctgatag attgcacaac atgagaacat taaaatacat gcctccagaa aaagcaaagt
    18181 ataaagcaaa agagacactt gaaatttatg gtggtatagc tcatagatta ggaatttcca
    18241 agataaagtg ggaactagaa gacagagcat taagatttat ggacccagaa gggtattatg
    18301 atttagttag tagagtatca atgaagagaa gtcaaagaga ggattatatt caaggaattg
    18361 ttgaacttct taaagataag tttaaagatt taagtatacc atgtgatgta tatggtagac
    18421 caaagcattt ttacagtata tatagaaaga tgcaaaaaaa acataagact tttgaagaga
    18481 tttttgattt aacagcagta aggatattgg ttgataatgt aaaggattgc tatgcagtac
    18541 ttggtatagt tcatacatta tggagaccta ttccaggaag atttaaagat tatatagcaa
    18601 tgccaaaacc caatatgtat caatcattac acaccactgt tgtaggtcct gatggagaac
    18661 ctttagagat acaaataaga actcatgaga tgcataatat agctgaaaat ggtatagcag
    18721 cccattggaa atataaagaa ggtatctcta gcaaagagtc aaaggttgaa gaaaaacttc
    18781 aatggttgag acagatgatg gagtgggaaa aagatttaaa agacccacaa gagtttatgg
    18841 atgcacttaa agaagatgta tttaatagtc aagtttatgt atttacgcca aaaggagacg
    18901 taattgaatt accagcagaa tcaacaccta tagattttgc ctatagagtt catacaaatg
    18961 taggtaataa atgtgtagga gctaaaattg atggaagaat agttcctatt gattataagc
    19021 ttcaaaatgg taatattgtc gaggtactta catcatctaa ttctaatgga ccaagtaggg
    19081 attggcttaa tatagtgaag accccaaatg caaaaagtag aattagacaa tttttcaaga
    19141 aggaaagaag agaagaaaat gttgaccgtg gaaatacaat attagaaaga gaatttaaga
    19201 agcatgatat accacaaaaa gaccctcttg ttgaaaaata tatggcatta gttgctaaga
    19261 aatttaatca gccaagtgtt gaggacttaa ttgccactgt aggttatggt ggtataatgt
    19321 catctcaagt ggtatctaag gtaaaagacc tatatttaaa agaggttaaa aaagctaaaa
    19381 aggaacaaca gatacaagaa gaagatttaa ataaacacaa tataagtgaa acagaataca
    19441 gtaaaaagag aaagaaaaac gcatcacaag gaattatagt aaaagggctt gacaatatct
    19501 tagtaagatt tgctaagtgt tgtaatcctc ttccaggaga tgaaattgta ggatatatta
    19561 ctaagggtag gggagtagcc atacacagag ctgattgccc aaatgcaaac aataatggtg
    19621 atttcttcca aaatagattg gtggaggttt catggagttc agcaggaaaa ggtaagtttg
    19681 aagcggaaat tcaaataaaa gctattgaca gaagaggaat tgttaatgaa ataactcata
    19741 ttgttgcaat ggataaactt agtttaaacg gaataaatgc aagaaaaggt aaggatagta
    19801 ttgtaagtat aaacttactt gtagaggtta atgacacaga ggaattaaaa attctaatga
    19861 aaaaattaaa agcaattcct ggtgtagaaa gtatttatag aatgacaaat tagagttaaa
    19921 ataataggga ggaaactatg agagcagtag tgcaaagagt atcttcatct aaagttacag
    19981 tagatgaaaa cactataggt caaataaata aggggttact agtattgtta ggagtgactc
    20041 atgatgataa atcaagtgat gtagattata tgattgataa aatattgaat ctgagaattt
    20101 ttgaagatga aaatgacaag atgaatttat ctctaatgga tataggtgga gagcttttag
    20161 tagtatctca atttacttta tatggagatt gcagaaaagg aagaagacct ggatttagta
    20221 atgcagctag accagaactt gcaaataacc tttatgaaga atttgtaaaa aaagcaaaag
    20281 ataaaggtgt gacagttggt acaggtcaat ttgcagctca tatgatggta gagttaacta
    20341 atgatggtcc agtaacaata ctattagact caagtaaatc tttttaaggg ggaaaacttt
    20401 aatgattata aaaaaatttg tagatagtta ttttggagta aatacttatg tattaggtga
    20461 tgaaaagact aaaaaatgtg cagttataga cccaggtgga agtttagtag agacattaag
    20521 ttttgtaaaa gaaaatgaat taaatattga atatattatc cttactcatg ggcatggaga
    20581 ccatatagga tatgttaagg atattaaaga aaaaactggt gctaaagtag ttgcacatgt
    20641 tgatgaaaaa gaattattaa atgacaaaaa taaaaatcta agttatacaa tgagatgtgg
    20701 agcgcaagag tttgatgcag atatctatgt aaatgataaa gacaagcttg aacttggtga
    20761 attaaaaatg actttcttgc atactccagg acatactcaa ggatgtatgt gtataagagt
    20821 tgaagatgaa atgttcacag gagatacttt gtttgctgga agtattggta gaactgattt
    20881 atatagtgga gactttaacc aaatggaaaa atcacttaag aaattatgta aatatgaaga
    20941 tgctgttaga gtatatcctg gacatggacc aaccagtact ctaggggttg aaaagaagac
    21001 aaatccttac atgtaatttt atattagtat ttaaaaaagt ataaaatatt taataactat
    21061 attagtgagg gggttcttct actgttaaat agaaggagtt aagttgatgt tgtatgtttt
    21121 tttaaaagga catgattata agtatgaagt agcagaactt ataaaattgt ttactagtga
    21181 atttaagttt aaagactcta atgctaacaa taatatagag tcaaagcatt taataaatag
    21241 attgatttat gagaatggtg tattattctc aagtactgaa tactatgaaa atggaaactt
    21301 aaagtacgag tcaatacaga atatagaaaa tatgaatctt gaatttttag atggatttga
    21361 tagcagcaaa acgattgaag aatttcttaa tagcctagat agtgaaagta agaggaactt
    21421 taaaaagctg tgtaaagaaa atatcaaaaa aagcatgttt ttagtgctta aacaagtatt
    21481 taattcttat gtaccatggg gaattttgac tggtattaga ccagtcaaaa ttgtccacaa
    21541 tttaatggat aaaaagctag atgataatag cataagaacc attctaaaag ataattattt
    21601 tataatagat gagaaaatag atttagctct tgaaatagct aaaagagagc gattatttat
    21661 gtatcctata gataaaaata aaatttctct ttatgtaagt attccatttt gtccaactag
    21721 atgtgtatat tgttcatttc cttcaaattc attaaaacaa tttggacatt taaaaagaga
    21781 atatgtttat aaattgattg aagaaattaa ggggtttgcc aaagttatta aggatactaa
    21841 gaaagaagtt gaaactctat atattggtgg tggaactcca actacacttg atgaggaaga
    21901 attggatttg cttataaatt cattgttcaa tgaattagat ttaagtaaaa ttaaagaatt
    21961 tactgtagaa gcaggtagac ctgacactat tacagaacaa aaactaagag tgttaaaaaa
    22021 acacaatgtt agtagaatta gtataaatcc tcaaactatg aatgatgata ccttggtaaa
    22081 gattggaaga gagcatagag tatctgattt agtagattgc tttaatatgg ctcgtagaat
    22141 gggatttgat aatataaata tggatataat tttaggtctt atagatgaag acttaaatat
    22201 ggtaaagaac acattggagg agataaaaaa actttctcct gaaagcctta cagttcatac
    22261 attagccata aagagagcat ctaacttaaa tataaatatg gataggtata aagaacatct
    22321 aacacaatat gaagaaatgg taaaaatgat agatttatca atgaaatatg caaaagatat
    22381 gggattaaat ccatattata tgtacagaca aaaacatatg ttgggaaatt tagaaaatat
    22441 aggttatgct aaagaaggat atgagtgcat ttataatata cagattatgg aagaaaaaca
    22501 aagtaactat gcattaggtg caggtgctat atcgaaattt gtctatgtag atgaagatag
    22561 aattgaaaga gtagaaaatg taaaaaatgt tgaacaatac attgaaagag tagatgaaat
    22621 gataaaaagg aagaaagaag aggtatataa aaatgttgac taaagctcct agaggaacta
    22681 aagacataac tccaaaagag gcttacaaat ggcgttatgt agaaaataaa tttagagaaa
    22741 tatgtgcttt atatggatat gaagaaatgg taacacctat atttgaacat acagaacttt
    22801 tcaagagaag tgttggagat actacagata tagttcaaaa agaaatgtat tcttttaaag
    22861 ataaaggtga tagagaaatt accctaaagc cagaaggtac agcaggtgta gtaagagcat
    22921 ttatagaaaa taaattatat gcagatactc aaccaacaaa attattttat gtgacacctt
    22981 gttttagata tgaaagacca caagctggta gacaaagaca atttcaccaa tttggaatag
    23041 aagcacttgg aagtgatact ccttcaatgg atgctgaaat aatcgcacta gctgttcaat
    23101 tttttaatga agttggatta aatgatttgg tagtgagtat aaattcagtt ggctgtccag
    23161 tttgtagaaa agagtacaat gctttattaa aagagtatct tgactcaaaa gctgatatat
    23221 tgtgtgatac ttgcaatgag agaagagaaa aaaatccaat gagggttata gactgtaaaa
    23281 atcctacatg taaagaaaat atcaaagata taccatttat tgcagaccat ttgtgtgatg
    23341 attgtaaatc tcactttgat aaacttcaag aatacttaaa ggaaatgaat ataaactttg
    23401 taattgataa aacaatagtt agaggcttag attactatag aaaaactgct tttgaaatta
    23461 tatctaatga tataggagct caaagtactg tatgtggtgg aggaagatat gatggtcttg
    23521 ttgaacaatt aggaggacca aaaggtataa gtggaattgg gtttggacta ggaattgaaa
    23581 gacttttact tacacttgaa ggtaatggaa ttgaaataga aaaccctcaa tctacggata
    23641 tatttatagt aactataggt gaagaagcaa atacaagaag ctttaaattg ttaaaagact
    23701 tgagacaaaa tcatataagt gcagataaag accatataga gagaagtgtt aaagcacaat
    23761 ttaaatattc agataaaata aattcaaaat ttacaatagt aattggagat gatgagctta
    23821 aaaatgacac tgcgacatta aaaaatatga aaacatcaga gcaaactact gtaaaattaa
    23881 gtacattagt tgaagaatta aagcaaaagc tgtaaaataa gaaagtatga tgttttgtgt
    23941 attagataaa tatatagcat taaaattaaa taaaatggag gtagtatctt ggaaacttta
    24001 aagggtttaa agagaactca ctactgtgga gagttgagag aaaaaaacat aaatgaagaa
    24061 gtcgtactta tgggatgggt acagaaaaaa agaaatttag gtggtcttgt atttgttgat
    24121 ttaagagata caagtggatt atgtcaaata gtttttgata cagatgtaga taaagaagct
    24181 tttgaaaaag ctgaaaaatt aggggctgaa tttgtaatag ctgtaaaagg aaaagtatgc
    24241 gaaagacaat ctaaaaaccc taatatgcta actggtgata tagaaatatt tgcaactgaa
    24301 cttagacttt taaacaagtc agaaactccg cctatttata taaaagatga tgatgatgtt
    24361 tctgaagcac taagattaaa atatagatac ttagatttaa gaaaaccatc tatgcaaaga
    24421 aatttaaaat taagacataa agtaatgaat attacaagaa attacttatc aaacaataga
    24481 ttttgcgaaa tagaaacacc atttttaata gctccaacac cagaaggagc aagagattat
    24541 ctagtaccaa gtagagttaa tccaggaaaa ttttacgcat taccacaatc accacaatta
    24601 tataaacaat tattgatggt aagtggtatg gatagatatt tccaaatagt taaatgcttt
    24661 agagatgaag acttaagagc agatagacaa ccagagttta cacaaataga ctgcgaaatg
    24721 tcttttgttg agcaagaaga tgttatgagt atgattgaag gtttgcttga agcgatattt
    24781 aaagaagttt tagatgtaga attggcactt ccacttccta agatgactta tgcagaagct
    24841 atgtctaaat atggttcaga taaaccagat actagatttg gatatgagtt aacagatata
    24901 tctgatgttg tttgtaactg tggatttaaa gtgtttgctg atgctacaca accaggaaaa
    24961 agtgttagag gtataaacgt aaaaggcaaa gctgacgact ttactagaaa acaaatatca
    25021 tcactagaag aacatgctaa gacatataga gcaaaaggtc ttgcttggat gaaagtagga
    25081 caagaaggag taacttctcc aatagcaaaa ttctttaatg aagaagaaat gaacgctata
    25141 cttacaagaa tgaatgctga agtaggagat ttacttttat ttgtagctga taaaaactca
    25201 atagtatttg atgctttagg acaagttaga cttgaagtag caaataggct taatctattg
    25261 gataaaaatg tatataactt actatgggta actgagttcc cagtttttga agaagatgaa
    25321 gaaacaggca cattctcagc aatgcatcac ccatttacat cacctatgga tgaagattta
    25381 gacaagttag aagaaggtga taagtcatct ttaagagcga aagcttatga tattgtattg
    25441 aatggttatg aaataggtgg aggaagtgtt agaatatcaa attctgatgt tcaatcaaga
    25501 atgtttaaag cacttggttt tacagaggaa agagctaatg aaaagtttgg ttatttatta
    25561 gaagcattta aatatggtac tcctcctcat gctggtctag cttttggtct tgatagactt
    25621 gttatgttac ttgcaggaac tgataatata agagaagtta ttgcattccc taaaaatcaa
    25681 aatgctgttt gccctatgac aaatgctcct acattggcgg aagatgagca attagaagaa
    25741 ttaagtataa aagttgatat aaaagataat gaataatatg tagttgcaag aaactaaaat
    25801 gtaaaaagtc tggatataaa atatatttat attcagactt tttttattag aattatttta
    25861 tattttttct ctatatattt tcataaatta tacttatatt cttaaccaat ataattaaaa
    25921 tactatgttt aaaaatttta cataaacttg attattttag aaactacttc tgtaatgagt
    25981 acacgatttt aattaaagaa tatataacaa atttgaaata agtctaatat atgacttaat
    26041 agattaaatt aaaataataa atttggacta tttatctatt tataacttaa attatatcaa
    26101 attgataaca aactagatat tatacttttt tgtagaatag aattacaatt atataaagaa
    26161 aaattctatt aatagtgaaa aaaaagtaga aaaaagtaga aattattctt ttataaagaa
    26221 agggagggat aatattgcaa gagagagaag aattagtttt aaatccagtt ccaaaagagg
    26281 aaagagttgg atggttagct ccattgttta atatgcttgg ttctaacata gctatatctg
    26341 aactaatggt tggaggaact ttaattttag gaatgactct ttctaatatg attattacat
    26401 caattatagg gaacttaata ttagttgcaa ttattatgat acaaggttat ataggttaca
    26461 aagaagggtt aaatacgtat gtattagcta agggggcatt tggagagata ggaggaaagt
    26521 atcttatatc tctattactt ggtataacta gttttggatg gtttggtgta caagcagggg
    26581 ttgcaggact atcagtacaa aagatatttc caagtgtaaa tctaacatta gttacagtta
    26641 tacttggatt atttatggtt gtttttgcac tgtatggctt taaagctatg gctaaattta
    26701 attacttagt tataccacca cttatgatat taatggtgtg gggagttttt aaagcttttt
    26761 ctacttatgg agttgaagct atttataatt atactcctca aacaactatg agtatggttg
    26821 aaggtcttaa tattgtagta ggtcttgtta tagttggtgc aattatatca cctgaccagt
    26881 taagatatac gagacgagta aaagatattt ggattatcag ttttttaggt ctagggatta
    26941 tttcattatt ccaacaagtt gcagctggtg taatgtctat gggagcacct acttgggata
    27001 taacagaagt attggctaat ttaggttttg gatgggtagc gtttgttatt ttaatacttg
    27061 catcttggtc tacaaacgta tcaaatgctt attctggtgg attagcatta aaaactattt
    27121 ttccaaatgt aaaaagaaat gttttgactt tagttgcagg tctaataggt acaataatag
    27181 cagctactgg tataatattt aaatttcagt catttttatc tttcttggga atcgcagttc
    27241 ctgcaatagc tggaattatg tggtgtgagt attattttat acaaggaaga acttataagc
    27301 atagagaagg aataaattgg attgcagtaa taagctggtt aataggtttt gcagcgtcgt
    27361 attatagttc aaaaataaat ttcttaatac ctccgataaa tggaattgta gtgagtatgg
    27421 ttatttatta tatattaatg aaatgttttg gaatcaaaga taaataaagg gggcaattac
    27481 atgggtcgta aggtgaaaat aggtataatt caacaacaca gtgtacttgg agatgttaaa
    27541 aaaaatatag aaaaggcagt agagatgata gatgacttag gaaaacaagg ggcagatata
    27601 atttgtttac cagaactatt tgctacagga tataatttag agtctttagg tggagttaaa
    27661 acattagaat taataagaga acataataag tacatagagg aatcaatgtc agaagctgct
    27721 aaaagaaata atgtatacct gatatctcca tatggaacct tagaaaaagg gtctacacat
    27781 gtttataatt cagcagttat atttgataga aaaggtaaga ttatgggaga atactgtaaa
    27841 aatcatctgt ggtcattaga agcagtatac tttaaaggag gggaaaaagt agaggtatat
    27901 gatgctgatt ttggtagatt tggtgtaatg atatgttatg atgcaggttt tccagaagta
    27961 tccagagaat tgacattaaa aggttctgaa ataatattta taccatctgc atggagaata
    28021 caagatgaag atatgtggga tttaaatgta tctcaaagag ctttagagaa cactgtatat
    28081 acagtaggtg taaatttagt aagtaatgac tctaatttaa tactatttgg aaagagtaaa
    28141 atatgcaatc ctagaggtac tgtaataact caattagata catacaggga aaaatatgta
    28201 ttatcagaga ttgatttaga tgaaataaaa aagtataggg aagatatccc ctatttgaaa
    28261 gacagaaaaa gtagctataa catataattc tatactataa tacaagttca atataaaatg
    28321 ataaaaagag gatgtatcaa tattaagatt aaatttatta tactttgata tatcctcttt
    28381 aatattatca actttgattt aaatattttt aaaatagttg ataagaaagt ttttgaacaa
    28441 caacacagct ttagattcat agttttcgct aaaccatgaa agatttaaat atcttttaaa
    28501 ttcaatatct ttgatatgta acaacttaat ctcagggtct tgagataatc cccatgattt
    28561 tccacatata aatccaactc cttgaagtga ttttataaga gcataaattg tatatggtga
    28621 atcagactca aatgctattt tgggtttgaa attagctgat tcgcacagga tatcaattac
    28681 ctctctataa ttctcaccct tcgttataac tataaagttt tcatctgaaa cttcgcttag
    28741 atatacttca tcctttaatg ataaaggatg gtttattgaa actcctaata gtatttcttc
    28801 gcatgttaaa gttatgctat tttctgagtt tagctttgta aatgaagaag atatgtacaa
    28861 atcaaaatca ctttttttat aagatgatgg caaagtatgt gacacattaa aagtaatatt
    28921 gggatatagt tttctgaaat tactcaaaag tgatggtaac acattagccg ctgcaggaga
    28981 taatatcttt acttctcctg aattttctaa agaagtgtct tgtaactcct tttttgaact
    29041 ttctactaaa tctaaaatgc tatctactct ttttagaaac aatcttccat actcatttaa
    29101 aacaatatat cttccttttc tgtcaaataa ctgaactcca agttcttttt ctaaggaaga
    29161 tatagttttg cttaaagctg gctgtgctat atttaacttg tgtgatgctt ttgttatgtg
    29221 ctccattcta gcaacagttt gaaaatactt aagatgcaat aaatccatag tataaaatac
    29281 acctctttta atatgacttg agttatatca attttataat caactccaca ttatacatta
    29341 taatataaag tttatataat aaactacaat atattaaaaa gttatttaaa t
//
LOCUS       DAMJWS010000046        29117 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMJWS010000046 DAMJWS010000000
VERSION     DAMJWS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.046"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            213..2855
                     /gene="adhE"
                     /locus_tag="PC399_002163"
                     /gene_synonym="adhC"
     CDS             213..2855
                     /gene="adhE"
                     /locus_tag="PC399_002163"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2593020.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3215..3556)
                     /locus_tag="PC399_002164"
     CDS             complement(3215..3556)
                     /locus_tag="PC399_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593021.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3583..4254)
                     /locus_tag="PC399_002165"
     CDS             complement(3583..4254)
                     /locus_tag="PC399_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593022.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4266..6842)
                     /locus_tag="PC399_002166"
     CDS             complement(4266..6842)
                     /locus_tag="PC399_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2593023.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVILANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7050..7985
                     /locus_tag="PC399_002167"
     CDS             7050..7985
                     /locus_tag="PC399_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2593024.1"
                     /translation="MANNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRVHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8004..8741
                     /locus_tag="PC399_002168"
     CDS             8004..8741
                     /locus_tag="PC399_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593025.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(8927..13984)
                     /locus_tag="PC399_002169"
     CDS             complement(8927..13984)
                     /locus_tag="PC399_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF2593026.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(14009..14428)
                     /locus_tag="PC399_002170"
     CDS             complement(14009..14428)
                     /locus_tag="PC399_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593027.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14662..14913)
                     /locus_tag="PC399_002171"
     CDS             complement(14662..14913)
                     /locus_tag="PC399_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593028.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            15002..15733
                     /locus_tag="PC399_002172"
     CDS             15002..15733
                     /locus_tag="PC399_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF2593029.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15792..16703
                     /locus_tag="PC399_002173"
     CDS             15792..16703
                     /locus_tag="PC399_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2593030.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKFIEAGKFELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16754..17566
                     /locus_tag="PC399_002174"
     CDS             16754..17566
                     /locus_tag="PC399_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF2593031.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17675..18367
                     /locus_tag="PC399_002175"
     CDS             17675..18367
                     /locus_tag="PC399_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2593032.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18454..19395
                     /locus_tag="PC399_002176"
     CDS             18454..19395
                     /locus_tag="PC399_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593033.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLNQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19432..20589
                     /locus_tag="PC399_002177"
     CDS             19432..20589
                     /locus_tag="PC399_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2593034.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20741..21301
                     /locus_tag="PC399_002178"
     CDS             20741..21301
                     /locus_tag="PC399_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2593035.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21329..21820
                     /locus_tag="PC399_002179"
     CDS             21329..21820
                     /locus_tag="PC399_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593036.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            22049..22600
                     /locus_tag="PC399_002180"
     CDS             22049..22600
                     /locus_tag="PC399_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2593037.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22686..23618
                     /locus_tag="PC399_002181"
     CDS             22686..23618
                     /locus_tag="PC399_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF2593038.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23673..24536
                     /locus_tag="PC399_002182"
     CDS             23673..24536
                     /locus_tag="PC399_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2593039.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24783..26645
                     /locus_tag="PC399_002183"
     CDS             24783..26645
                     /locus_tag="PC399_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2593040.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26648..28114
                     /locus_tag="PC399_002184"
     CDS             26648..28114
                     /locus_tag="PC399_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2593041.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQRKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            28138..28974
                     /locus_tag="PC399_002185"
     CDS             28138..28974
                     /locus_tag="PC399_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2593042.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10765 a         3502 c         4667 g        10183 t
ORIGIN      
        1 gtaaaagtaa aaaaaataat atatttgtta aaaaataaat tgacaaaata ttcagaaaaa
       61 aacttcttga cctaagagag taaaataaat aaaatctaag gtatagagat gaaatctata
      121 aaattatttt aagtataagt taaaaatata gatgatgcat ttaagattaa taaaaattag
      181 agatgcataa ttattttagg gaggttttta gtatggagaa aaaagaaaag gtagtagaaa
      241 aatcaaatgt tgaagtatgt agtcctgaaa ttgtcaacag tgttgaaaca ttaagaatga
      301 gattagaaga aattagattg gcacaaaaag aatttgcaac atttacgcaa gaacaggtag
      361 ataagatttt cttagctgct tcaactgcag ctaatcaaca aagaatacct ctagcaaaaa
      421 tggcagtgga agagactggt atgggtattg ttgaagataa ggttataaaa aaccattttg
      481 cctcagaata tatctataat gcttataaag atactaaaac ttgtggtgtc atagaaaaag
      541 atgaagcatt tggttttaca agaattgctg agccagtagg tgttttatca gcagttatac
      601 caacaactaa tcctacatca actgcaatat tcaaatccct aatagcacta aaaactagaa
      661 atggtataat tttctctcct catccaagag ctaaaaactg tactatagaa gcagctagag
      721 ttgtacatga tgcagcagtt aaggcaggag caccaaaagg acttataggt tgggtagatg
      781 ttccttcgat tgaattaact aatgttgtca tggcagaggc agaccttata ttagctactg
      841 gtggacctgg aatggttaaa tctgcatact cttcaggtaa accagctgtc ggagttgggc
      901 ctggaaatgt acctgcaata atagatgaga gtgcagatat aaaaatggct gtaagttcaa
      961 tcttagtatc aaaatcattc gataatggta tgatatgtgc ttccgagcaa gctgtaatag
     1021 taccagaaaa aatatatgaa gaagttaaaa aagaatttaa atatcgtgga gcacatttcc
     1081 taaataaaga agaaactgaa aaagtaggaa aagtagtaat aattgatggt tcattaaatg
     1141 ccagaatagt tggacaacca gcacatgtaa tagccaaaat ggcggatgta gaagttccaa
     1201 aaacagctag aataataatt ggtgaagtag aatctgttga attaaatgaa ccttttgcac
     1261 atgaaaaact ttctcctgta cttgcaatgt acaagtctaa aagttttgaa gatgcagttg
     1321 ctaaagcaga aaaattagtt gcagatggag gctatggtca tacatcttca ttatatgcag
     1381 actctataaa tcatccagag agagtagaaa agtttgtaaa tgctatgaag acttgtagag
     1441 tattagttaa tactccatca tcacaaggtg gtataggtga tttatataac tttaaattgg
     1501 caccgtctct tactcttggt tgtggttctt ggggtggaaa ctcagtatct gaaaacgttg
     1561 gagtaaaaca tttgcttaat atcaagacag tagctgaaag gagagagaat atgctttggt
     1621 tcagagcacc agaaaaggtt tatttcaaaa aaggatgttt aggtgtagca gcaagagaat
     1681 ttaaggatgt tatggataaa aagaaagcat ttatagtaac agattctttc ctatataata
     1741 atggatatac taagaaatta actgatttat tagatgagat gggaattaag catactacat
     1801 tctttgatgt agctccagac ccaacattag cttgtgcaag agaaggagct aaagctatgg
     1861 ctgatttcca accagattta ataatagctg taggtggagg aagtgcaatg gatgctggta
     1921 agataatgtg ggtaatgtat gagcatccag aagtagattt ccaagactta gctatgagat
     1981 ttatggatat aagaaaaaga gtatatgtat ttcctaagat gggtgaaaaa gcgtattttg
     2041 ctgctatccc aacttcagca ggaactggtt cagaagtaac tccatttgca gttataactg
     2101 accaagattc aggtgtaaag tacccattag cagattatga attaatgcca aacatggcta
     2161 taattgatgc tgatatgatg atggagatgc caccaagatt aacagcagca tcaggtgttg
     2221 atgctttaac acatgcatta gaagcatatg tatctatgtt aagaactgaa ccagctgacg
     2281 gtctagcact acaagcaggt aaaataatat ttgaatattt acctcgtgca tataaaaatg
     2341 gaaagaatga taaagaagca agagaaaaaa tggcaatggc atctactatg gcaggaatgt
     2401 cttttgcaaa tgccttctta ggaatatgtc actctttagc acataaatta ggtgcattcc
     2461 accatgttca acacggtgtt gcaaatgcat tattaataaa tgaagtaatc aagttcaatt
     2521 gtgcagaagc tccaaataaa atgggtgcat tctcacaata cagatatcct gattgcatac
     2581 aaagatatgc tgaatttgct tcttttgcag gtattaaagg aagtacagac caagaaaaag
     2641 tagataatct tataaaagca atagatgaac taaaggcaaa agtaggtctt cctaagacta
     2701 taaaagaggc tggagtagaa gaatctaaat tcttagaaag attggatgct atggttgaac
     2761 aagcttttga tgaccaatgt acaggtgcta atcctagata cccattgatg agtgaattaa
     2821 aagagatata tctaaaagtt tattatggta aataatatct attgtgcaac ataggttcta
     2881 ttgggtttgt atataaatta ttagatttga atataaatag agttttatta gagacacgta
     2941 gaagtaacta taaataatag attataactt aaattctaaa tggaaatgat aaaaagtcag
     3001 taatttaatg gtaatttcga atatgtgtat ttaatcaatt aactttaaaa ttagtcggaa
     3061 aattttttta ttacatataa ttttaaagca taatatataa aaaactttat gaaaaaatta
     3121 taaaaatatt cagaaaattc tattgactaa tttatttatc atgatataat aagtttagaa
     3181 aagaaaaaaa ttggggccta ttaaggcccc cttactattt tttgatagat ttatttaaaa
     3241 ttactttctc attttgattt tttatattta ttatagagtc agtataatcc acttcaaagt
     3301 tggcaaaagc agagaataaa tctatatctc ttaacaaatg aattgtcata cattgattgt
     3361 tcttatttag ccttagaatc aatgctctta caaatggaaa ttgtattcta gaattaaata
     3421 agttagaacc atcagtatct atatctaagc tacgataatt aatatctata aatatatcta
     3481 catcatcatt ttcttctctt attattctgt agtcaaaatc caagtttaaa tttaaattac
     3541 ctaaaatcaa taacatttaa atcctctttt ctataaatag ttttagagtg ttaatttaag
     3601 ctctccatct attatagata taattctatc tgcttttcta gcaatatttt cattatgtgt
     3661 tattattata agggtttggt tatatttttt tatagaactt tttagtaaat ccatgacttc
     3721 ttctgtagtt ttactgtcca aatttcctgt tggttcatct gctaggatta tacttggttt
     3781 tgcagataaa gcacgtgcaa tagcaactct ttgctgttga ccaccagata attggttggg
     3841 aagatgattt ttcctttcat taagacccaa taaatttata aggtcatcaa tatattcttt
     3901 atctattttt ttattgtcta actttagtgg taattctata ttttccttta catttaatac
     3961 aggaattaag ttatactgtt gaaagacaaa tcctaccttt tgtcttctat atcttgctaa
     4021 ttctttatct gaaagacaac atatatcttt accatctacc tttatagtac cattacttgg
     4081 tctttcaaga ccacctacta aatgtagaaa tgtacttttc ccacttccac ttggtccaat
     4141 tatagcaaca aattcgcctt tatttatttt taaatttgca ttctttagtg catgtacaga
     4201 tgtttccttt ttcccataaa tcttacctaa attaatagtt tccaatatag gcataattat
     4261 ccctcctatt ctgtaatgtt tatagcttca actatactaa ttttatttat ctttcttgat
     4321 ggaatatatg tagctagaat accaacaatt atatttgcta aaataactag gatatatatt
     4381 ttgtaatcta ttgaaaatcc caatccatat gatacaaagc cctgcatata atacatttta
     4441 aattgtatta taattcctga cacgatagta attatacttg agagtacacc atataatatt
     4501 ccttcataaa gtatcatatt tttaaagcct ctctcagaga taccaatagc tcttaacatt
     4561 ccaaattcac tagttcttga agttaagtta taacttatgt tgtttattat gttaagtaca
     4621 ctgattatga acattattgc tactatacca taactataaa tcatagttct agatgtcatc
     4681 ttatcagaat tttctttttc taccatcata tctactgttg tagttccagg aacttcactt
     4741 ccaattttac ctaaaaggtt atttattttt ttcttgtttg cattttcatt aaggtctgca
     4801 taaactaagt tatacttatc tacaccagtt aattttttta ggtattcgct actcgttatt
     4861 acatctacac catcatctcc agagtacata ccattatctg caaaaggata acttataata
     4921 gctccaactt taaattcata ttctaagtat tcataattat cttttagact ttcataaagc
     4981 cctgtatcca tcccaccctt tggatatttg acttttatag tatcacctac ttttatatct
     5041 acagctggtg ttccttcacc aggagtaaaa gtctttgtgt atttctgtgt tttagaaatt
     5101 tctggtacat atacaatagc taaattttct tttttcattt ttttcaagtt tatatttcca
     5161 cttactaagt atttttctaa tgactttatc atctcatcat tataaccttt tagcttttgc
     5221 tttagtgtat atttgccagt agtcttattt tttatgaaca gaggatttag cttataataa
     5281 ggagttgaat ttatagcttc gtagtaatct aatctattta ttttatttac ctctattctt
     5341 gtgtttaaga tactagcagt tttgactttg tcaattccat ctatattttt tattttatct
     5401 atctgattat cattaaaccc ttttatatct ttaaaagaag tattgttacc aatttcattg
     5461 agataaacat ttgatagaat aaaatctgca ttcatatatg tttgttcttg agtagatttg
     5521 ttttgcacat ctaaaaaaga atatttataa ttgtctttta tgacgattat acctcctata
     5581 ctcatagata atacaattat aatgaacccc tttttatttc taaagatgtt tttcgcagat
     5641 atagattttg ttactgacat atttaaagtt aattttgata ttaaataatt aactttggtt
     5701 acatttttat cagctccaaa agtttttctg atagcatcaa taggagaaat tcttcttatt
     5761 tttaagtatg taaagaaact tataattagt attattagta ttgtacatgc aattgataag
     5821 agaattattt tatctggaat aacaaaaggt gtaacattta tatttccttc aaattgaata
     5881 tttcctacag acctattaaa tatttgtgca cctcccatac ctatacatat acctataggc
     5941 attgctatta gagcaagtat gaaaagttca taaagcataa gtttgaaaat cctaaagttg
     6001 gtggaaccaa tagctcttaa gacgccatat tcctgtactc tttggtatat tgatataaca
     6061 tagatactat atattacaat accagcaaat agactcatag caattgcaac gtatttactt
     6121 gaattatcta aagtaccgtt tttcatagta gattctataa gcattgagtt tcttcctata
     6181 cttttctcat ctaacatagc attttttatt atattgtcta tgtttgtatt tatatcactg
     6241 ccttcattaa attcaatata agttgtaaaa tcattcagtt ctgattcatt gagattagaa
     6301 aaaatttcac attgtccctt ttttttctct ataaatctat cttcaaggat tccaacaact
     6361 ttaaaagttt ctggattttc tttttgatat aatttaaaag taaggttttg atttatctct
     6421 ggttttaagc ccatactatt taaaatccat ttctcgacta ccacctcatt gctagctttt
     6481 ggaaatcgac cttttataag tttagaatga ctagtcaaat aatttttatt tgcatatagt
     6541 atgtttatag gtaatttttc attaacatct gtagacgcat agtaagatgt tatacccaga
     6601 tttttaatat ctttgctaga ttttatggac tttaattgat ttttctttat atttttaaag
     6661 tatacatggt cagttccaag ctctcttttc aatctatcta catctgcttg ctgagcactt
     6721 cttgagaggg taccaatacc aacaacgagt gatgtagcaa gaattatact cacaacaatt
     6781 gctaaagacc tagctttata actttttata taccttagta ctattttcaa attatctttc
     6841 atatttacca cttcctttct taactaaatc aattatatat ttatctaaaa tatatagcaa
     6901 agtaagtatt ttcaagtttt aagtagttaa ttgtacaaat tcatctgtat ttttacaagt
     6961 ttaattaatc tatatgtaaa ttttaacttt ttacataatt tttttaaaat gatataatcc
     7021 ctatataaat tgtagagggg agggaaatca tggctaacaa ttttgtattt cctatagtaa
     7081 tactaacttt gtttatattt agcaatctta taatgaataa gtataaattt tacaaagaat
     7141 tagacatatt aaaaagaggc atattaacat tttttatatc gtgttttgca ttgattttat
     7201 ttatggaggg cactaaatat atacaattaa ggttaggata tagtttttta cttgaaggag
     7261 tattaaatat tatacttttt ttggttttaa ttattttaag tttttataca ggctttaaag
     7321 ttataagatt gataaataat aataatgaac aggaatataa actacaaaaa ctagatagta
     7381 aaataaatca taaggataat ataattatca aattaagaag tcaaaaacat gactatttga
     7441 aacatttgca agtggtgtat tctctattaa atactgggct agatgaagat gccaaagaat
     7501 atataatgaa tatatcaaat agttttaaat caaaaaattc aaggtatgga aaagtatctt
     7561 atttagatgc tatagcttca ttaaaatacg aggaatgtct tgaaaaggat atatcttttg
     7621 atgtctacat tcaagatttt atggataatt tagagattga acctaatgat atgagttcag
     7681 tactattaaa cataatagat aatgcaatag agtctttgtc tactataaaa aaagaaaaga
     7741 agtatataag agtacatgca tatgaagatg aatttcagta tattatagct ataaaaaata
     7801 atggtcctaa aatacataat gttgaaagta tattttggaa tggatttagt actaaagaag
     7861 gacaagatag gggatatggg ctacatttag ttaaaaatat attagataag tatgattata
     7921 gtatacttgt aaatagtgat gattttttaa ctgaatttgt aatcttaatc ccaaaatatt
     7981 attagttagg aggagaaaag tctatgataa aagttttgat ttgtgatgat gataagattg
     8041 taagagatga tatagcaaat atagctaaag agtctggata tgtagattat gttaaaaaag
     8101 ttaaggatgg tatagaggca attgaattta taaaaaatga gaaaatagat ttattgatta
     8161 ttgacattga tatgccatat aaaaatggga ttgattctgc tgaagaaatt ctttctatag
     8221 ataaggatat acatatagta tttgtgacgg gtttcgctga ttatagtctt gaaagcttta
     8281 aagtgcatcc tatagatttt gtagttaaac catttaaaaa agaaaaaata cttgattcaa
     8341 ttaacatagc aatagaccat attaactccc ataagattgc taataataat tttatagaag
     8401 acaccctttt tgtatataaa ataaggaagc agatacatat gattaatttt gatgatatag
     8461 ttatgtttga gaaaaattca agagcaatta atctacacac taaagatgaa gatataataa
     8521 agttttatga gaactttgat gatttaaaaa aacgcctacc tcctaatttc tttatgaccc
     8581 atagacaata catagtaaat ctaagattta tacataaggt tatacctata aataaatctt
     8641 cattagagat aaggtttata aattttcaag aaaatgccat attaaacaaa tctctagaaa
     8701 aagatttttt atatagattt tatagggtta aaagattcta gtgaagatag aagaaaaata
     8761 ctttttgtgt gatataataa gtaccataaa gataatgtat aagagaagtt tatataaaaa
     8821 ttatttgtaa ttatgattta atctatataa attacagtat agtatatatt ggactttcta
     8881 atttttttta agaaagtcca atatatttta gggatagttt ttatacttat aaattactta
     8941 agatactagg gtctttaatc ttactatctt cagcaagtaa tagtatctta gacataatct
     9001 cagcagtttt agggtcttca tcagcgaatg gaaggaatat cttacctcta ctttgagaat
     9061 gtataggaag tatattcaaa gcacccatcc cttttttatg tacaatacca gaacccatat
     9121 gcacagaata ctcacctaaa ttacctgcta taagagcatg agagccttca aaattgacat
     9181 tgttaatttt taataataat aaaagttctt tagcaattgc tatacgcatc tctacagtag
     9241 aatggctagt ttcagggtca acaccaccag catgagcgac acttacaaca aggtctatat
     9301 cacgcataac ttctgagaat agaattggag gtatagaagc taattctaaa cttttataag
     9361 tttttctatc aaaaaattct actgtttcaa tagtaggagg ctctatatct gctggtgaaa
     9421 accaatctgc aagtgcaaac atctttgcca ctatattttc tttataaaat actttctgta
     9481 atccctcttc ataatcgaca gtccaaagct gatttctaag aagtgctaca gttttctttg
     9541 gctgaatttg gtgaccagca tatctgcgag atatagtttt ttcagataat tcatcactgt
     9601 ttattctata atactcacgg aatacttgct taaatggttg aacaatttgc ttactaaata
     9661 aatcatgttg gaactcactc cacttaccag attcaaacaa atgaactgga tgtgcaagtt
     9721 ttaatttatt ttttgccgtc aacttataag tttcaccttc tatattgcaa ataaagaact
     9781 tagacttatc attataaaca tatccaatat tatcatcaca tacccatact agatttttaa
     9841 tcaaagggca gataactgga tttttataga tattggcaat ttctttaact gtaaatacta
     9901 tttcattttt catagcttgt tctaaactct gttttgcacg agaatactga ctctttaaat
     9961 ctttttggac agatttaagt tcaagtacat atggatgttt agcaatagct tttggtatac
    10021 tctttaaaga cttgttgcct ttttgaactt tagtagatgc ttttccgttt tcatcaacat
    10081 ctatccaaat tgaagtacca tcaatatctt ttggtttaaa tagatgagca acttcattta
    10141 gtttttcagt ctcaagagcc cacatcatac gatttacatc tccaaatcca gcacttctag
    10201 ctagattttc tagcgctata gtacaagctt ttgattcact ttcacgacgt tgtgcaccaa
    10261 attgcttgct ttctttagca aacttttgaa taaattcata acggaaagtt gcttctttta
    10321 ggtctccttt tttcatagga agtaaagaga aacttctcaa tttatcttga tttctatttg
    10381 tttcaatttc tttcatcaac atgtctttat caagcttacc aagtgtagca tcagcataga
    10441 gttgtgcacg tctatgagag ttaccaccac tagttatata tttagctgac ttgtaaagta
    10501 tattgaatct ttcttcccca atagttttat atgaatctaa aaaccaatct acatcaaatg
    10561 caccatcaat gaaactacgt ggtgaaatag gggagaatag agcaacctct gattcttttt
    10621 gtgcagagaa gccttcattt atatgtgcat ggaagtacca aattgtactt ttcagaccct
    10681 tccagtttaa atatttttca attatgttta cccattgagg ggcatacata gcagattcta
    10741 tcaatcttaa ctcagtaatg tcggtatttt taagtaaact ttttaatttt tttgagtcat
    10801 cttctttagt aggatagcaa tttttaagta gagaacttaa aacatctttt tttgaataat
    10861 tgctaccata tgaatatcct ctctggaacg tttctttacc aagagcgacc aaaatattta
    10921 caaaataatt tataccttca aagtattgta tattattagc tagatgagac acctctgttg
    10981 gcatatcacc tctacgtgat tcaatttcta taattcggtt tgtaacagtt tttaaattat
    11041 ctttaagcca agggtacttt tctaatgtgt cgccagccca tctaggattt gtcaatgttc
    11101 ttattatatt tgatgcgttg tatccactta tcatttctct attaacttgc tcttcagtca
    11161 ttaatcctaa ttcatgtgca cgagcaaaca catttatgct agtatagaaa ggaaacttat
    11221 attctagcaa ctgataattt gaaaacgtag tgtagaaaaa cttagtaaaa gaaatatcat
    11281 cataaataaa cgaatgtgct ttatcaagcc aatagtttaa aataggggca tttaaagcag
    11341 ggcagtattc attttgtctg taatagtttt tagtaaattc aactctttgt tttttacatt
    11401 gttctatagt caataagttt ctaagtttat agtatatttc taaacaatag ttaaatattt
    11461 ctttatggtc aaactcattt agtaaaactc ttaaaagttt tcttgtaagt ccattaaatt
    11521 taagtttttc aagttttgta tatattttat tttgtttttt agagtcaagg acatttgaat
    11581 atatagatga aaaccaactt ttttctgttt cattatatcc acatgtactt atactataaa
    11641 ataaaatatc aagtattaat ttgtaatcta agtcaagctt tttactagtt tctctccaaa
    11701 gctctgctaa tggataatta tcaagagtat cattagaaac tttatattta ataggagtca
    11761 agttataagt agaataacct aaaagtactt tgtctacatt acctgaccat ctttcaactt
    11821 catattcata atctgcattt tcagtaataa gctcatcaaa tttttcataa acttttatta
    11881 ttttatttat atccgtatcg agaatttttt ttgcttcttt ttttgttgct acagaaacag
    11941 aatcctcacg agtagataaa cttatatttg agataatttt attaagcttt gatgagctat
    12001 tagaatcatc tttaaaatca aattgttctg gaatttgaac tttgttttca gggttatata
    12061 ttccaaaacc attttctttg cagtaatcat tttttgcttt acctaacaaa ttatcaatta
    12121 gtatttgaac ttgagatgta ttctttttag atttatcaat atcttctata agtaaaacaa
    12181 gcttattata tagttcttgt ctagcagtat tgtcttttaa taagtttaca atttcaagac
    12241 ctccaaggca tttttgagaa tttgaagatt ttatcagtga agaagcaata ttttccaaat
    12301 ttgaatcatc ttgttcaagt aaaattttta ttactgtttg tctaatagag cttgtcttta
    12361 aagacaatag attggttatc ttatctaatt ctacatctgt tagctcaagc tttctaagta
    12421 gactcaatac cgtttctcta ttggaaacac tcttatcact taaacattcc aataaaaatt
    12481 cacgttgtct atctgtcttt ggttcattta caaaattgac tataagtgaa gctcttaaat
    12541 ctgaactcat aagatgacaa ttttccataa gcacatctat aaattgagca ttcatatcgt
    12601 atgctgttaa agtcatcata catttaagta tttcatgaga agaaatttca atccaagtcc
    12661 atggaaatac acttcctgaa aatgattttg tctttgaatt taaaatttct aaagtatgtt
    12721 ttaaactttc aaattgttct tttctagcat caaagtcttt gtcaatagga gtgtttgaca
    12781 agtcaaaatt agtttctctg ttttgccaac tatagtagta gccaacagaa ccttctacat
    12841 agaaatttgt aattacccat gctagtatct ctaaatcatc acccttaatg tttttagatg
    12901 ctatagaatg tttaaaatat gtactttctg tatgcgataa aaagtaaaga gctacaagtt
    12961 ttttatattt ttcctgacaa gaaagtaagt tttcaataag agagtaagag tcatatatgt
    13021 tgtaagttgc agtagcccat aaggcaaaat aaatttcaag attatcattg ctattaattg
    13081 attctatacg gaacttttca tcagtcaatg atttatatga aagttctaaa catttacgta
    13141 ttattccagg tttttggtct gaaaaaccaa gtcctgtcca tgtatccaag gctcttataa
    13201 cagaactaaa tcgctctaaa ttgttgtcca aaatagtttt taacatgtat attgatgcag
    13261 aaattgtacc ctcatctaaa ctctctacaa tttgctgtct aagtccttct tgttgtttgg
    13321 ctgccaaaag aagtttagaa agcatttcat agcatttatc actatgactc ttcatgatac
    13381 caattatcat ttgtcttgta agtagagcag tttgattttc tccaaatatt acttcttcta
    13441 aagcacttat tactttttga tttcctttgt ctatttctag tgctatttta tcactcatta
    13501 tagagtcata gtacaagacc ttatttggaa gagtaagata atctataact gaaaaatcat
    13561 aataaattaa atcaacatag tttgaaataa accttataat acctctaaaa taatccacaa
    13621 gtttatttga gcgataccct ttacgataat atcctcctga atatgtataa tccatctttc
    13681 ttttaattaa ttggcaaagt tcttctactt gttcagaagt aaatattaat tctaatgcat
    13741 tttttaaagg tccagaaagg agctcgtcca tatttttaat ttcgtagtag tggcaattgt
    13801 agtatttctt tatcatattt aagaatttgt cttcaggttc tctataatag tatgaactat
    13861 tttctatgca gtcccaaaga gactttgcta agtcgtagta ctgttttgaa gaagatttag
    13921 taaaattatt tttaaaattt tcaattattt taggattttt ttctactcta gtacgttcta
    13981 acatttatgt acctccaaat tatttatttt aaaacattct tccagcaagc aaggtaagtc
    14041 ttataaaagt aaatatatct ccacttttca gatttataga ggaatcgagt ttttcaataa
    14101 catcttcatt tataagcact ttgtagattc catcttcaaa tgcctgtaac gctacctcta
    14161 ttgccttttc tacagattgt ttattctcat tgtaaataga gccaaatcca acttttccaa
    14221 tatcagcttg agattcaatt tcttcattag ttaagaagtt tattattgat ttttctggga
    14281 ttttctcatt atattcaaga acatttttag atacaatttc agagatagct tcttttaggc
    14341 aattgatgtt gtcagataca tcaaaagggg taagttctag cataggtttg cgtttgccaa
    14401 cagccttaac tctaaaataa atttgcataa aatcacctcg caaaatatta attatgtatt
    14461 tagaaattta aaaatttatc tatttgttat attatatcat tttgtgaatt attaatgtga
    14521 attatggaat tattttacaa aaaatagaaa aaaagctatc ttaaagtcta aaaaagcaat
    14581 aaaagataac ttttaactat aattatatag aattataact gtacatttat taaattttaa
    14641 ttttagaatt atttttgata actatctttt tttatttata aattttgctc ctaaatattt
    14701 tttgatatct tcttcaggag aaaagtcatc aataagtatt tctttcccag tgtcttcaaa
    14761 tactacaaga acttcacagt caccttgtaa gaaaaatttt ctaactgtat cttctttaac
    14821 ttctctaact tttattacat tctttttttc tttttcaaag tcttttgtgt aataacttcc
    14881 catcatatta attatttttg tatatctatt cataaaatac ctccatatgc gattaaattt
    14941 aaaaaatatg ttataataat tgtttgtatt aacttaataa ttataacaag gagtaataat
    15001 aatgactagt ataaaaaata aagatatttt aaattgtata gattattcca ttaaaaataa
    15061 gctctttgac aaactaaatg atgtatataa gtccttgcca gtaggaaatt gttctggatg
    15121 tggaaattgt tgtatggagt cagtaggtat aaatttaatt gagtttttga atatattttg
    15181 ttatcttgaa gatagagttg atttaaaaaa aagctgtata agtaaaatat tagattatta
    15241 ttttgaggag tatacaagaa agaacccatg tccatttaaa gatactaata atagatgttt
    15301 aatatatgaa gttagaccat taaattgtag gatgtttgga cattggaaaa aagaggatta
    15361 taataaaaat ttagataatg ttattgagaa aaataataac tatagagagt taatgaaaaa
    15421 gcaatatgga tttgaaataa atgatgaagt agtaaattac aggatagatt attgtgatag
    15481 atttattcca aataaagatt atttaagtaa atctaagaga ttaagctttt ttgatgagct
    15541 tatgatatta gactcaaata tttattcaag tggaaatata gatatagact ttagggatag
    15601 aggtatagtt gaatatttta tagagtcatt actttataga aatttagcat ataatgtgaa
    15661 aataaggata tctaaagaat acaaaataag aaaaagaact atagatagaa taaaaaggat
    15721 tgttcttgta tagttttata ttagtaagta ataaattgta tcataaatat tgtcaaaatg
    15781 aggtgaagaa ggtgaatctt acaaaagtaa aaggaaatac cttttttata aaaggtggca
    15841 cgaatacagg aatatatgta tttgaagata acacagcact tatgatagac ccagggttat
    15901 gtggttcaag acctaggaga gtaaataaag tattagatga aaataatata caggttagat
    15961 atattataaa tactcatggt catgatgacc actatggagc ttgtaatcaa tttaaagaat
    16021 attataaaga tgtgtgtata atgtcatctg aatacgataa attatatata gaaaatccag
    16081 aattgttttc aaagtatatt gttgggggaa agagcaatgt atttatggat aatatcttaa
    16141 aaaataaaat gttggatgat ataaaaatag ataaatttat agaagctgga aaatttgaat
    16201 taaacaatga aatgtttgat ataattgaat ttcagggtca tacacctggt agtatagctg
    16261 ttttgacgaa ggataatgtg atttttgttg gcgatttatt gataggagat gaaattttat
    16321 caaaatacga tttcttgttt ctatatgata tacaagaaca aataaattca ttagaaaaat
    16381 taaaaaatat agattttgag tacttagttt tagggcatgg caagcaagtt atatcgaaag
    16441 aagattctta taaattaata gaaaaacatt tgaatgcttt aaaaaaatat ttatatcaaa
    16501 taagagagtt gcttgtagaa ccgaaatctt tagaaatttt attaaaaaac attataaata
    16561 acaataattt aagcaataat tataaagagt atcatttttt taaatcttcg ttagtatctg
    16621 tcataagtta cttagttgat ttagaagaaa tagggtatat attggaaaat ggagaattgc
    16681 tttactatac aaaaacaaaa taaattatga taaaataaag aaggtcataa aaaacaaata
    16741 aagggtgagc aatatggaat acgaaaaatg gaatgagata ctagcacctt atgaacatgc
    16801 agtagaggaa ttaaaaataa aatttaaaaa tataagaaaa gaatatttag caaaaggtga
    16861 atattctccc atagagtttg tcacaggtag aacaaaaaaa atatcttcca taatatctaa
    16921 gttaaaaagg ataaatgcaa aagatataga aactgaaatt gatgatattg ctggtataag
    16981 aataatgtgc cagtttgttg aggatatata tgctatagtt gatttgataa aagtaagaaa
    17041 tgatatgact ataataggag aaaaagatta tattacaaat tacaaagata gtggttatag
    17101 aagctatcat gttataataa aataccctat aaactccatt gcgggttcaa aggaaattat
    17161 atgtgaaata caaataagaa ctcttgcaat gaatttctgg gctacaatag aacattcatt
    17221 aaaatataaa tatgaccatt acatacctga gactcttgct gaaaggctta gaagagcatc
    17281 tgatgctgcc tttcttttag accaagaaat gagtgaaata agagaggaca taatgaaagc
    17341 tcaggctatg taccaaatga agtctatagc tattagagat actctaaata gaatacaaga
    17401 gttatatgac ttgggtgata cacataaagc tatgcagtac caaagaaaac ttgatagaac
    17461 tgaaacagat aaaaatataa ctgaaatttt ggaattaaaa caagaaatag atatattatt
    17521 agagcaatat aaggatacta aggtagatga taaagtagtt cagtaaaaac tacttgtaga
    17581 aaatttgcta aatattaatg gggctgcctc ttaatggaaa ataatttcta aaaagagaca
    17641 gcctatttag tgtaattaag aaaggaatga tattatgagt ttgtatgcta taggtgattt
    17701 acatttttca acatcagtca ataagccaat gaatatcttt ggaagtaatt gggatggtca
    17761 tgaaaagaaa attatagata attggaaaga agttgttaag gaagaagata tggtcttagt
    17821 actaggagat acatcttggg gaattaattt atccgaagct aaaaaagatt tagatataat
    17881 aagcaaactt ccaggtcaga aaatacttat aaagggaaat catgattact ggtggacaac
    17941 cgttacaagt ttaaataagc tatatgaaga catgcgtttt attcagacaa atttttatga
    18001 atataaagat tatgcaattt gtggaggtag gggctggata tgtcctaatg atgttaaatt
    18061 tgatgagact gatgaaaaag tttataaaag agaagaacat agacttagac tatcattaga
    18121 atctgctcgt aaaagtggtc actcaaaaat aattgtgatt actcattatc ctcctacgaa
    18181 tgataaattg gaagaatctt tatttacaaa gttatttgaa gaatataatg ttgaaaaggt
    18241 gatttatggt cacttgcatg gtaaagaatc ttttaaaatg ggattgaagg ggattcgaaa
    18301 tggagtagaa tatacattag catcttgtga ctatacagaa tttaatttaa taaaagttca
    18361 cgattaaaaa acataaattg gtaaatacta taagatacat gattaaaaat aaatatttga
    18421 gggatttcgg ttttgagaaa ggagacacga cttatgaggt ggatattagt ttttatattt
    18481 ttatatctta ttcctttagt agtgatattc agaaattaca agaattttaa gaggtcttgt
    18541 atctacagta gtatttatgt ggtattagct acaacaataa tgataagtaa tatttatatg
    18601 agtggactta ataaaataaa agagtctatg tattatcaca attatatttc tgatgagaca
    18661 tatgatgaaa agtatgtatc aaattttaag gaaaaagaaa gtactgattt aaaccaggat
    18721 aataaaaaga atgaagaaat atcaaataat aaaaatttaa acaacaaaca agatagaaat
    18781 atacaaaata aagaaagtaa tgaagaagga agtaatgata taaaagaaaa gaataattct
    18841 gatagtaatg tttcagtgaa taataaatca gaaaaacaag aagataatac aaaagaaaat
    18901 aataaatata caagtaaaga aaaaaatagt gaagatataa aaaaaataga tatggaaagt
    18961 ataggaattt ttaagaaaga aatttataat atagaaagag cagctttagt tccaatgaga
    19021 gaatgcatac cttacactaa ggatatagca aaaaatataa ctaaattatc tagtataaaa
    19081 aaagatgtta caaatgccag aaacaagtgt aaagaagttg taaaagaata tgaaaatatg
    19141 cagattccag aactttctaa ggagaagtat acagaaacat tgagtaatgc tagagatgac
    19201 gtaaagaaag catatgagct tagagaaaaa tctatggaaa gtgctttgaa attaataaat
    19261 actaaaagcc ctaaatatat agggaagata acagaatatc ttgatttatc agataagcag
    19321 atagaaagtt ttaaaaatag attgaacgat ttaaaagaag aaataaataa ttcagaaaca
    19381 aataatatta attaataaga tttaaggata taagttaaaa gggagattat gatgaataaa
    19441 cagagtatag gaaaactaaa tatgtttttt atggggatta ctcaaagaaa ccttgataat
    19501 agggattttt ttattgagtt agaggtgatt tttaaatctg gaagaaaaga gtttaaagga
    19561 attgcaatag aggaagataa taaatataaa tttaatttta aaggaaaatc agatttatat
    19621 acgtttaatg agttgcttaa gaatatttct aaagaggcag aaaattatga tggattagta
    19681 tttaaatatg ttgagcgagg aactattgtt gtaattgaag gagacaataa gaaggtaaat
    19741 gtaaaatatt tagataataa ggaagaagta cctaaaatag atgaatttac ggcatctcaa
    19801 ataaaaaaca gagattatta tgtaaaagta ggacaagcaa atgcactttt aaaagaaatt
    19861 ggagtactta caaaagatgg taagataaaa aatgataaaa taagaaaata caatcagata
    19921 gaccattttg ttgaattgat agatagtatt ttaaaagaaa taaaggataa agactgtata
    19981 actatcttgg attgtgcttg tggaaaatcc tacttaagct ttgtcttaaa tttttatata
    20041 aaagaagtat tgaagaaaaa ctgttatttt ataggaatag attattcaga tgtagttata
    20101 gaagcatcaa aaaatatggc taaaaacttg ggttataaaa atatgtcatt tataaaagag
    20161 gatttgacta attatacacc taatagggat gttgaccttg taataagtct acatgcttgt
    20221 gatacggcta cagatatggc aataggtcta ggaattagag caaaatctga agctatagta
    20281 gtagtaccat gttgtcataa ggagctatta gggcaatata gatatgaagc tatggaacca
    20341 atattaaaac atggtgtatt taaagctaga tttgcagatt taataactga tggtttaaga
    20401 acattgttat tggagggcaa tggatatgat acttctgtag tagaatacat atctccactt
    20461 gatacaccaa aaaatttaat gataagagcg ataaaaacta aaacgaataa tgacaaagca
    20521 ttaaaagaat ataaagaact gaaatcacaa tttggagtgg aaccaacctt agaaaaactt
    20581 atatattagt taaaaatgta aaatttacga aaagcttgct taaatatgca agctttttgg
    20641 tattatatag gtatagtcat atccagagtt ttgatttata attaaagctg tgctataata
    20701 tttgattaat caaatcaata aatgttacat ggaggtaaaa atgaacaaat tgaatatatg
    20761 tattgatata gatggaacaa taacaagccc ttatcatttt cttccatacc taaatgatat
    20821 ctttaataaa aatataactg aagcagagtg tataacacat aattgggaag aattgtatgg
    20881 ttcgggaata agagatgtgt atgcggagtt taataataag tatatacact catatgatga
    20941 agcaaagatt gtagagggtg ctacagaggt aattagcaca ttatctaaag gacataattt
    21001 atcttttgta acggctagac atgagtgttt aactgatgta actaaaaatt ggctatcgag
    21061 acaaggattt tcagatatag atgtttactt attaggaagt gattataaag ttgaaaaggc
    21121 aagagaactg agttgtgaca tatttataga agataatcct ttaaactcag tgcaactagc
    21181 tgatgatggt gttaaagtaa tacttctgga tactaactat aataaagatg ttaaacatca
    21241 aaatatagtt agagttagta attggataga tattgaaaag attataaaac aaggtattta
    21301 aagatttgta gattgaaagg gggagcatat gatagattta aaaattgaaa atagaaaagg
    21361 aaaaatccat gtaaagagca gtgaaataaa agacatttta gaattacgtc cagacttcga
    21421 atatgtacaa gatatatcaa atactataaa tcaagaaaat atattggtat ttgattgcca
    21481 gttgacagga aattcttttg atatggatga tttagatata gaagaaatat tggaagagtt
    21541 aggtgaagaa atagatgagt cttatttcag tgttttattt gaagatgtaa gagcgtattt
    21601 aaaagatgct actgatgaga tagaagcaga cttacaggat aattacttat ttgataattt
    21661 gagatgttat tttgatatat acaatataaa tcaagaattt acagatttta agtttgtatt
    21721 tttagtttct tttaaagata taaaaatttc ttcattagca aacctagcta agattgtttc
    21781 taagagacag cttataggtg catctaagtt ttatagttaa aatgaaagat tattataata
    21841 aaagctagta accttgtaaa aatataggtt actagttttt tatttattca ttttcacaaa
    21901 atttcagaaa actatttaca ttctaaaaaa agtaatgtat attaaacata atgtacatta
    21961 taatggataa aaagtttcta ttaaattact aagtttattt aataatcaaa ataatttatc
    22021 ttatattagt atgtattagg gggtatttat gcaaagttta aatgaaataa ttgctgaaaa
    22081 tttaaaaaag ataaggaaag aaaaacatct tagtttagac aagattgcac aacttagtgg
    22141 tgtaagtaag agtatgctat ctcaaattga aaagtgtgag gtaaatccta ctatatctac
    22201 tttgaagaaa ataactaatg ggctaaaaat ctcatttaca tcattgatgg agaggcagga
    22261 aagtgacata gaattgattc aaaaaagtga tatagaccat tttattgaag ataatggaaa
    22321 gtatataagt tatccaatat ttccatttga ctccaaaaga agatttgaga tatttatgat
    22381 tgaaatagaa gaaggtggaa atttagattc aaatgctgaa cttccaggaa cacaagagtt
    22441 tataactgtt tttagtggcg aagtaaccat taaaataaat ggagaagatt acattgtatc
    22501 tagtggaaat tctataagat ttaaagctga tgtatcacat atatatagaa actcaggcaa
    22561 aggtatagct aaaatgagta tggtagtata ttatatataa ataatttaat ggaattagag
    22621 tgatatttag atgattttag gtatgaagat atattatatt taagttatag gagtgtgatt
    22681 tatggatgaa taataaaaaa gcagtttttt tcttttgcag tgatttaaag aaagacccag
    22741 tagccagtaa tgtgataaaa tgttgtgaag aaataatgga tttaaatcaa actgatattg
    22801 ttattgatga taattttgta ttagagttta aggataaaaa caataactta tttcattttg
    22861 taaaaacaaa agatgtaata agtcataatt ataaatatta tcttccaatt ttaaatagac
    22921 attttagaga ttatgatttt gcaggagttg taaattggca tgagggagaa aatgctccag
    22981 aacatatcct aacagctcat acaattggag atgttccaac aggagaattt ggaaattcaa
    23041 atccaagata ctttaaaaat ctaatcaatg ctattgagga tataaaaaat gaaaatctac
    23101 ttgatgaatt tactacatta acagaagcaa ctcactggtc tggaacaaca tatggtgaag
    23161 aatctaaatt aattacagaa tattctgtac ctatgttgga tattgagata ggaagtagta
    23221 gtgatagttt taataatagt atagcaatac aggtattggc aaaatcatta attagagtat
    23281 ttgattgtga tgaacctttg aagactttgt tatgtgttgg aggggtacat tttgagaaat
    23341 ctttctcaga tattataaaa aataaagaat ataacatatc aattggacat gtattgccaa
    23401 atcaatggat tgtaagtgga atgtatgatg atgaatctgg atttgagaaa ctagaaaagt
    23461 gtataaatag tattgaagga ggaattgatt gtatagtttt tcatgataaa ttaaaaggta
    23521 cctataaaga gcaatgtcgt aaactaggtg aaaaacttaa tgtacctgta tttaaacata
    23581 aaatattaaa aaatccaaaa gacttaccta tatggtagtt ttatacagtt aatataacaa
    23641 taagtcggat taatttttaa gcgaggtcaa aaatgatatt atcttatttt gcattattaa
    23701 ttagagtagt gctattagct tttgaaagag tagttgtaag attattaggt gatgaagaag
    23761 gagatattta taaaaatata gcttcatcat ttctattttt ctttataggg ggagtatgtt
    23821 tacttccatt tagcataatg gatagagtta gtgattggtc gtttttaata ccatgttata
    23881 taagtagttt agtctattca attggttcag tagcgtatgt aacgtcttta gcaactgggg
    23941 aagtttcact tgttactcca ataaatagtt taaattcatt gtttttactg cttttatcag
    24001 ttatattttt aggtgaaggt ttaagtttag caaaagttgc aggaatcata attatgattg
    24061 gtggagtgtt tatattaaaa aaagtaagct cttttttaaa gaatgtctct gtaattttta
    24121 acaatttacc atgtagatta atgtttttat acatattttt gcagtcagta ggaagagtat
    24181 tggataaggc tttttttgta caagtatctc ctatattata ttcaactata ctgtattttt
    24241 tcgtaggttt aaatttattt atctttttag catttaaaaa gaaacaaaaa ataatttgtg
    24301 agatttttag taataaaaag gggctttcaa ttttaagtgg tgctattaat ggatattctt
    24361 atttagcatt attaattgct ttaaataatc tagaacttag tatagcagag ccattttctc
    24421 aagtatctat gataataact ttaatacttg ctcattttat attcaaagaa aatataaaag
    24481 aaaaaatacc aggttcaatt ttgatactaa ttggtgggtg gctactgtta ctttaattga
    24541 tataaaaaat gaattataaa ggtgctatta gttagtatca gaaaatttaa aaaagtattg
    24601 acaattcttt taggcaaatg ttaatataat aatacaaaat agtataaata gaagacatgt
    24661 tactggtaat gcaggcagga tcgaatatat tgctttgatt tttcaaggtg tttatatagg
    24721 ttctgccttt tttgcatctt gaatggccat tcataaaaaa ttatggagaa ggagattaca
    24781 agatgaatta taaaaaaact gccattgaca ttattgaaca agttggtggt tcaaaaaata
    24841 ttttagcttt agaacactgt tcaactagac tacgctttaa gttgattgat agaagtaaag
    24901 tcaatcagga tgctttaaaa aaagtacaag gtgttatgag tatagttaat ggggcacaat
    24961 ttcaagttgt cattggaaat agtgttgttg atgtgtatga tgaaatttta aaagtttgcc
    25021 ctttagatac aaatgatagt actgtaaaaa ctgaagaaaa aataaactta ggaacaagat
    25081 ttatagaatt tattattaat atttttcaac cactgatttt tgctattgca ggagctggta
    25141 ttttaaaatc actgttgatg ttattagcaa tgtttaatat tttaaaatca gattctacta
    25201 tatatacatt attagttgct atttcagatg caacattcta tttcctacct cttatggtag
    25261 ctgtaactac tgccaatgta ttgaaatgta atcgtttagt agctattgcc gctgtaggat
    25321 acttattgct tccagcaaca acaactgctt taagtgaagg tgtcgaacta tttggattta
    25381 caattccaaa cattgcctat aatgcacagg ttttcccggc tatcttatgt gtgtctttct
    25441 tggcaacaat ggagaaaatt tttaacaagt attcaccaaa accaattcga actttctttg
    25501 ttcctatgat gtctttggca attactgttc caattactct aactatttta ggtcctttag
    25561 gatataacgt tggtacaatt tttacaacca ttattttatt cttatataac aaaatgggat
    25621 tcttagttgt aggattactt gctgcttttc ttccatttat ggttgcaaca ggaatgcaca
    25681 aagcactaat accttattca attaatacaa ttggaaagtt gggttatgaa gctttatata
    25741 tgacatcttc attagcccac aatatttctg aaagtggtgc ttgttttgca gtagccttgc
    25801 gaacaaaaga tgaaacatta aaacaaacag cattatctgc aggaatttct gccttgatgg
    25861 gaattacaga gccagcttta tatggtatta ctttacaaca taaaagagca atgttaggtg
    25921 ttgtattctc aagtgcaatt tctggtacat ttttaggatt atttgcagta aaaggatttg
    25981 taattgttgg accagggctt gccagtatga gtatgtttat tgacccaaac aatggaaaca
    26041 atttgatttt tgcaagtatt ggttttgtta ttgcaatatt aggctcgttt attattacac
    26101 tgattatttg gaaggaagat acaaatacag tagagtcatc aattggagat gttgttgaag
    26161 aagagctaga agaagaaaaa gaagtaaata tcttagtaac tccagtagaa ggaaaagtaa
    26221 tagatttaag taaggttaat gatgaactat ttgcaagtaa gactttggga gatggagttg
    26281 caattatacc gaccaatgga aatttatacg caccttgtga ttcagaagtt gtgatgttat
    26341 ttgaaacaaa acatgcaatt ggtttaagaa caaaaaatgg ggctgagatt ttgattcata
    26401 ttggaataaa tactgtaagt atgaatggag atggatttaa aacatttgtt aagacaggcg
    26461 ataatgtaaa agaaggagat ttattaattc agtttgattt agacaaaatc tcacatgcta
    26521 atttagattc aactgttatg attgtaaaca ataatggagc agattatgct tataaagtgt
    26581 taaaccagtc ttatggcaat gtaaaaaaag gaagtatctt gtttgatgta aagggaggaa
    26641 tataaatatg agaaagtttc cagaaggatt tctttgggga ggagctacag ctgctaatca
    26701 atttgaaggt ggttggaatt taggtggtaa agggtggtct gtatcagatg tagcaaaggc
    26761 acactttgat gcagatgtga aagattataa atctaataat gaaatcacaa caaaagatat
    26821 agaagaagga ttagcacatc ctgaagatga agtaaattat ccaaaacgtc atggtagtga
    26881 tttttatcat cactataaag aagatattgc tctaatggca gaaatgggat ttaagacata
    26941 tcgaatgagt attgcttggt ctcgtatttt tccaaatgga gatgataaag agccaaatga
    27001 agaaggttta cagttttatg atgatgtatt tgatgagttg ataagttatg gaattgaacc
    27061 acttgttact atgtcacatt atgaaccacc attaaatatc gttttaaact atgatggatg
    27121 gtattcacga caagttatca atatgtttgt taggtatgta gaaactatct gtgaaagata
    27181 taaaaacaaa gtgaaatatt ggcttacatt taatgaagtt gattctatga ttcgtcatcc
    27241 gtacactact ggtggtttag ttcgagaccg ttttaaggat aaaaactttg aggaagttat
    27301 ttttcaagct atgcatcatc aatttgtagc tagtgccctt gcaacaaaaa tttgtcatga
    27361 aattattcca aattcaaaag tagggtgtat gttaacaaaa ttaacgtatt atccatacac
    27421 atgtagacca gaagatgttt tggcaactca acgaaaaatg agaagtattt atgcatatag
    27481 tgatacgcag gtttttggtg agtatccagt atatttactg tcgtatttta aaaataataa
    27541 cattcaaata gtaaaagaag aacatgatga tgaaattatg aaaaagtatc cagtagattt
    27601 tatttcattc tcttactata tgtcttcttg tgaggcagct gatacaactg gattagatat
    27661 tacacctggg aatacgttat tagctgtaaa aaatccttat ttagaaatga gtgaatgggg
    27721 ttggcaaatt gattcaatag gtctacgtat atctttgatt gaattatatg atagatacag
    27781 aaagccttta tttatagttg aaaatggatt gggagccaaa gatattttaa ctgaagataa
    27841 aaaagttcat gaccaatata gaattaatta tttaaaagaa cactttaaat gtatgttaga
    27901 tgcgattatt gaagatggtg ttgaactttg gggatatact tcatggggtt gtattgattt
    27961 ggtcagtgaa tcaacgaaac aaatgtcaaa acgttatgga tatatttatg tggatgctga
    28021 tgattatgga aaggggacat ataatcgata taagaaagat agtttttatt ggtataaaaa
    28081 ggtaattgaa agtaatagta tagattttaa atgaccaata gaaggtgagc aaataaaatg
    28141 aactacataa taaagaaagt tttgaactcg agcgttgttt tagttcatga cacaaaggat
    28201 aatgaattta ttttgctagg gaaaggaatt ggatatggta aaaaaacagg agaatatatt
    28261 tgtggttcca atgataatca aatgtttgtc cctgttgaaa atacaaaatc aaaacaattt
    28321 ttagaattaa tggatgatat tcctgtcgac attttaaaga taacacagga aataatagta
    28381 gaggcagaaa agttattgaa ctcatctttt aataaaaact tatacttaat attagcagac
    28441 cattttaact ttgcaattga aaggatgcgt aaaggaataa aaattacaaa tcgagtattt
    28501 tgggaaataa aaaattatta tccaaatgag tttaaggttg gaatggttgc aatttatatg
    28561 gtagaggaga gattgggcat tcaattacca gaggaagaag cagctaacat tgctttccat
    28621 tttgcaaatg ctatggcttc agatggaaat tcatatgata cgattaagta tgccaaagta
    28681 attggagaaa taattaatat ttttgttttt tctttgaata gaaccttaga taaaaagagt
    28741 atgcattata tgagatttat aacacatata aaattttttg tggaaagatt tttttcagat
    28801 agtatgttaa gcagtggtga tgatttatta tttaatcaaa tgaaaaggtc aaatccaaaa
    28861 gaaatgttaa ttgcattaaa agttagagat tttttagaaa agaaatatgg aaaaaagtta
    28921 acaaatgagg aaatagcttt cttggttgtc catatcgcac gagttaagca atagagtgaa
    28981 ttactaatat tatcttatac gtctacatgg tagaaaattg aaagaaggta aagtatataa
    29041 agaaactgag acatattatt ttgttgttca ttatgtttat tttatgagtg tatcctgtta
    29101 aaaatttaag ttaatat
//
LOCUS       DAMJWS010000047        29070 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMJWS010000047 DAMJWS010000000
VERSION     DAMJWS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.047"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            16..717
                     /locus_tag="PC399_002186"
     CDS             16..717
                     /locus_tag="PC399_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2593043.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            941..2242
                     /locus_tag="PC399_002187"
     CDS             941..2242
                     /locus_tag="PC399_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2593044.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            2379..3473
                     /locus_tag="PC399_002188"
     CDS             2379..3473
                     /locus_tag="PC399_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2593045.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIMLLDFIEPWSKFKLILEGE"
     gene            3475..4608
                     /locus_tag="PC399_002189"
     CDS             3475..4608
                     /locus_tag="PC399_002189"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF2593046.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            4626..5735
                     /locus_tag="PC399_002190"
     CDS             4626..5735
                     /locus_tag="PC399_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF2593047.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            5800..6540
                     /locus_tag="PC399_002191"
     CDS             5800..6540
                     /locus_tag="PC399_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF2593048.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGYVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            6545..7537
                     /locus_tag="PC399_002192"
     CDS             6545..7537
                     /locus_tag="PC399_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF2593049.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVLICGIAMQLTDSIKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(7725..8606)
                     /locus_tag="PC399_002193"
     CDS             complement(7725..8606)
                     /locus_tag="PC399_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2593050.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            8879..10267
                     /locus_tag="PC399_002194"
     CDS             8879..10267
                     /locus_tag="PC399_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593051.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            10295..11248
                     /locus_tag="PC399_002195"
     CDS             10295..11248
                     /locus_tag="PC399_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2593052.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            11308..11532
                     /locus_tag="PC399_002196"
     CDS             11308..11532
                     /locus_tag="PC399_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593053.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            11525..12037
                     /locus_tag="PC399_002197"
     CDS             11525..12037
                     /locus_tag="PC399_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2593054.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQEDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            12069..12923
                     /locus_tag="PC399_002198"
     CDS             12069..12923
                     /locus_tag="PC399_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF2593055.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            13457..14251
                     /locus_tag="PC399_002199"
     CDS             13457..14251
                     /locus_tag="PC399_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2593056.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            14355..15161
                     /locus_tag="PC399_002200"
     CDS             14355..15161
                     /locus_tag="PC399_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2593057.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SITVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     AIHQLFNFLIGIINEDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            15223..15744
                     /locus_tag="PC399_002201"
     CDS             15223..15744
                     /locus_tag="PC399_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2593058.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            16159..18246
                     /locus_tag="PC399_002202"
     CDS             16159..18246
                     /locus_tag="PC399_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2593059.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            18261..18548
                     /locus_tag="PC399_002203"
     CDS             18261..18548
                     /locus_tag="PC399_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2593060.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            18575..19846
                     /locus_tag="PC399_002204"
     CDS             18575..19846
                     /locus_tag="PC399_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF2593061.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            19904..20503
                     /locus_tag="PC399_002205"
     CDS             19904..20503
                     /locus_tag="PC399_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2593062.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            20508..21413
                     /gene="dapA"
                     /locus_tag="PC399_002206"
     CDS             20508..21413
                     /gene="dapA"
                     /locus_tag="PC399_002206"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2593063.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPSKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            21519..22151
                     /locus_tag="PC399_002207"
     CDS             21519..22151
                     /locus_tag="PC399_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF2593064.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            22629..25136
                     /locus_tag="PC399_002208"
     CDS             22629..25136
                     /locus_tag="PC399_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2593065.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            25478..27640
                     /locus_tag="PC399_002209"
     CDS             25478..27640
                     /locus_tag="PC399_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF2593066.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNETTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRALNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            27645..28262
                     /locus_tag="PC399_002210"
     CDS             27645..28262
                     /locus_tag="PC399_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593067.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNKKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            28451..28795
                     /locus_tag="PC399_002211"
     CDS             28451..28795
                     /locus_tag="PC399_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF2593068.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT        11324 a         3141 c         5181 g         9424 t
ORIGIN      
        1 tttaagaggt gaatcatgtt aagaaatcaa ccattatata tacaagttgt agatatatta
       61 aagcaaaaaa tattaaataa cgaatataaa attgatgaaa atattccaac agaaagggag
      121 ttagagcaag agtttggagt cagtaaaatt actattagaa aagctattga gatattagaa
      181 tcagaagggt atgtagagaa aaaaagtgga aaagggacaa aagtaataag taatagtctt
      241 tttaataagc tatcaaaagc agagtctttt tcaagtatac ttttatctaa aggtcataag
      301 ttatctaaaa agactatttc aattgaaaaa ataataaatg atacaaattc acaagtattt
      361 agtatatttg gagaaaaatg tagcaagatt actagaatgt attacttaga tagtaagcca
      421 tatatatttt ttactcatta tattcctttt gatgaaaatc taactgttga aagttttaat
      481 aatgaaagtt ctttatatat gtacttatat aggagaaata taaatataac tagatttgaa
      541 gatgaatttt ttatagaaga atcagaacta gaggtatcaa aggctctaaa tctagctaaa
      601 gacatattat taggaagaaa aagaaagacc tatgatgaga atgataatat agttgagcta
      661 tcatttgcaa gatataatac agaagtacaa aactatgtaa ttaaatacga aatataataa
      721 gaatcctgag tttattgaaa tgataatatg ctcaggattt ttgtttagtt gtaaaaataa
      781 aaaatattat aacattataa tgtttacaac taaacattat aatgttataa tatttttaat
      841 aaacaagtga tattatcaat tacataaaaa ttagtttatg ttatgaaatc gttattgcat
      901 tataaataaa ggataatatc aataatacag ggggataact atggaaaaat tcgcaagttt
      961 tttagagcgc aagattatgc ctgtagcagc gaagataagt aatcagagac atatgagagc
     1021 tgtaagagca ggtataatag caacattacc actaacaata gtggggtcat ttttcacaat
     1081 attattaaac atacctattg atgcttatat ggattttata gctccattta aagcaatatt
     1141 agatatacca tttagatata ctgtaggatt tttatctcta tatgctactt ttgggatagc
     1201 atcatcactt gctaaaagct acgatttaga tacaacaggt gttggtatgc ttgcagttat
     1261 ggcattttta gtttctacaa tagttccaac tcaagtagtt gaaccagtag gtggagttat
     1321 agaagctggt agatggatgg atatggcaaa acttaattcg ccatcattat ttggagctat
     1381 cgtaacatca ttaattacag ttgaaatata tcgttttatg aaggaaaaga atattacaat
     1441 taagatgcca gcaggagttc cagtagaggt ttcaaattca tttactgcat tattaccgac
     1501 agcagtaatt ttaatgttat tctgggtaat aagacatatt ttaggatttg atataagtgt
     1561 tgcactaagc gctatgttat cacctttaaa aggattctta tcaggaaata gtttaggtgg
     1621 tgggttactg acagtattcc taataacttt attctgggta cttggaatac atggtccagc
     1681 tataatgggg ccagtaataa gacctatatg ggacatgtct atagcagaaa atataactgc
     1741 attcacagaa ggagtatcag ctcatgcaat gccaaacata tttacagaac aatttttaca
     1801 gtggtatgta tggatagggg gagcaggggc tactcttgca ttagttgtaa tgtttatgtt
     1861 ttctaagtca caatatctaa aaagtttagg tagattagga tttttaccag ggttattcaa
     1921 cataaatgaa cctataatat ttggagcacc aatagttatg aatccaatac tagggatacc
     1981 atttataatt ggacctataa taatgacaat tctatcatat gttcttacta ttacaggggt
     2041 tatacctatg atggtagcaa gattaccatt tgcaatgcca gcaccaatag cagcagtaat
     2101 gagtactaac tggagtatat tagcaggtat cttagtatta atcaactttg tgatatcatt
     2161 tgctgtttat tatccattct tcaaagtttt tgaaaaacaa caattacaaa gagaacaaga
     2221 agaaatgagt gaaaatttat aaaaatacat agatatagag gttttgtact atataaaata
     2281 tagaaaaaac ttataagtta ttaagggtga aggtaggagg aatgaccttt gcctttaatt
     2341 ttatatattt taaataatgt aatttgagga ggagaaagat ggaaagaaga ttaggaatat
     2401 cagtatatcc agagcatagt aacatggaag atgataaggc atatataaaa atggcttcaa
     2461 aatacggatt taaaagaata tttatgtgca tgctttcaat tacaagacct aaggaagaag
     2521 ttaaaaaaca ttttaaagag attataacct atgcaagaga attaggattt gaagtaatcc
     2581 ttgatatagc tccaaacata tttgatatat tacaaatatc ttatgatgat ttaacatttt
     2641 ttagtgagtt aaatgctagt ggaataaggc ttgactatgg atatgatgga agtaaagaag
     2701 ctatgatttc tttcaatcca tatgatttaa aagtggaact aaatatgagt aatgatgtag
     2761 catacttgga taatataatt acttacaagc caaatgtaaa taatatatat ggttgtcaca
     2821 atttttatcc tcaaaggggt acaggcttgc catatgattt ttttgtaaga tgttcaaata
     2881 gatttaaaaa gtatggaata agaactgctg cttttgttaa ttcaaaatat gcaacaatcg
     2941 gaccttggaa tataaatgat ggactgtgta cgcttgaaat gcatagaaac ttagatattg
     3001 ctacagcaac taagcactta ttcgcaacag gattgattga cgatgtaatt gtagggaatg
     3061 catatgcaag tgaagatgaa ttaaaaagaa tgtctgaagt aaataaatat caagttgtat
     3121 ttaatgtagt aactgaggaa aaaacttctg aagtagaaaa gacaataatg gcaaatgagc
     3181 aacactttag aagaggggat ataaccaacc aagtagcaag gtctacagaa gtaagaaaaa
     3241 aatatgctaa agaaaatttc cctaagcatg ataatacgac catctttaaa agaggagatg
     3301 tagtgattgg taatgatgac tttggaaagt ataaagggga attacagttg atacttcaag
     3361 attgtgcaga tgaaagaaaa aataaggtag gtagaataaa ggaagacgaa ataatgcttt
     3421 tagactttat tgaaccttgg agtaaattta aacttatttt agagggagag taaagtgaag
     3481 atagacatat taataaaagg tggaaaaaca gtagataaaa gtataattga tatagctata
     3541 ttagacgata aaataataga agtaaaaaat cagatagatg attctaagta tcaggcaaat
     3601 caaattataa acctagatgg agaaaaattt attagtgctg gttggataga tatacatacg
     3661 cattgctacg aaagactaga cctttataag gattatccag atgaggttgg aataaaaagc
     3721 ggtgttacta ctgtagtaga tgcaggaaca acaggagctt tagatatagg taaattttat
     3781 gaagatacta aaaaatataa aacaaatgta tatgctttaa taaatattgc taagcaggga
     3841 ataacatctc aagatgaact ttcaagtatg atgaatattg atgaatatga acttaaaaga
     3901 gcagttaaaa aatacaaaga ttttgttgtt ggaatcaaag ctagaatgag taaaagtgtt
     3961 gtaatttcaa atgatgttga acctttaaaa gtagctaaaa gaataaaaaa tgaacttaat
     4021 ctacctatga tggttcactt tgggagttct cctccaacga tagaggatat atttgactat
     4081 atggaaaaag gagatatatt aacccatata tataatggaa agccaaatgg aatacttaga
     4141 ggcaatgagg ttaaaaaaga aatcatagaa gctagagaaa gaggtataat ccttgatgta
     4201 ggtcatggaa ccgaaagttt tagtatggat attgcaatga aatctaagga tgcaggaatt
     4261 tttccagaca ctataagtac agatatatat attaaaaata gaataaatgg tccagtgtat
     4321 aatctatcta caacaatgga aaagtttata tatatgggct attcacttga agatataata
     4381 gataaagtta ctaaaaatgc agcagatgca ataagtttaa aaaataaagg tttaataaaa
     4441 gaaggatatg atgctgattt aactatattt gatgtggtta atgaagaaaa agaacttcaa
     4501 gattctttga ataagtcagt aatcacaagt acgtctataa agccaagagc agtagtagtc
     4561 aatggagaat atttaaatat aggtaagtct ataggagaga atgagtagaa gggagaaact
     4621 attgtatgag tagtatattt gaaaaatact ctgtaagacg tgttataaat gcttcaggca
     4681 aaatgactat actaggagta tcaacagtag ataaggaagt tattgaagca ataggtgaag
     4741 ggtgtaaaaa tttctttata atagaagaac ttataaataa aacaggtgaa tatatagcaa
     4801 atttattagg gtgtgaaagt gcactaatag tttctagtgc atctgctggg attgcacaga
     4861 gtgtgggagc aatgatagca aaagatgata tgagtctagt ttacaatgta aataatccta
     4921 agaaacaagt taaaagggaa ataataattc caaaggggca taatgttgat tatggagttc
     4981 cagtagaagt aatgataaga cttggaggag gagaagttgt tgaggccggc tatgctaata
     5041 tgtgtaacaa ggaacatata ataagagaga taaatgaaaa tacagcagca attatgtata
     5101 ttaaatctca tcattgtgtt caaaagagta tgctaagtgt attagatgca gtacaagttg
     5161 ctaaagaata taatttacct ttgatagtag atgcagcagc agaagaagat ttaaatgtat
     5221 attatgaatt gggagcagac ctagtgatat acagtggtgc taaggctata gagggtccta
     5281 gctcagggtt agtaataggt aagaaaaaat acatagataa tgtaaagctt caatcaaagg
     5341 gtataggaag agctatgaaa ataggaaaag aaaatattgt aggtctaaca tgtgcaattg
     5401 agagatatat aagtaatgat aaagtaactt taaaagaaat ggaggacaag ctaaatccat
     5461 ttatagacaa gataaatact ataaagggag tgagtgctag tataactaga gattcagcag
     5521 gtagagaaat acttcgtgga gaaattgact ttaatgaaga tataataaat aagtcaacac
     5581 aacaaattat aaatgaactt agaagtggag agatagctat atacactaga gactataagg
     5641 ctaatgaagg caagattgag attgatataa gaagtgtaac tggaagtgat ttagatacta
     5701 tttataatag gataaaaaat atagtagaaa gttagagtgt agagtaagtg acattataat
     5761 gatttaataa attaaatttt accaatggag gggataaata tgaattgtat accacaatat
     5821 tataaaaata gagtttgttt aaatgttctt gcaggaagca taaaaaatgc taaagaagta
     5881 tatgatgctt gtgatgggta tgtattgata ggagtactat caaagaatta cagtactgta
     5941 gaagcagcta ttgaagatat gaaaaaatac tcaaaggaaa tagataatgc aatatctgtg
     6001 gggcttggag ctggagaccc aaatcagtca aatatggtat ctcagatttc taaagaactt
     6061 cagccacaac atataaatca agtgtttaca ggtgtggcta cgtctagagc attgcttggt
     6121 caaaatgata gtataataaa tggattagtt tctccaactg gtaaaccagg atttgtaaaa
     6181 atatccacag gtccattaag ttcaaaagaa aaagatggta tagttcctat agaaacagca
     6241 atagctatga tgaaagatat gggaggaagc tcaattaagt tttttccaat gggagggctt
     6301 aaaacaaagg atgaatatat agaagttgca aaagcttgtg caaagcataa tttttattta
     6361 gaaccaacag ggggtataga cttagataac ttcaaagaga tagttcaaat tgcacttgat
     6421 gctggtgtag aaaaagttat acctcatgtt tatacatcaa taattgataa agaaacaggt
     6481 gaaacgaaag tggaagatat aggagtactt tataaaataa tgaaagaatt attgggatag
     6541 atttatgaat atattagctt ttggtgaaat aatgatgaga ctttcagtac ctgactacaa
     6601 gttcttaaca caaaccaatg aactaaatta tataattaca ggcacaggat taaatatatt
     6661 atctggactt aaaaactttg gatacaatac ctatatgttg acaaagttgc ctaataataa
     6721 tgtaggtaaa gcatcaagtg caaatataag aaagcttgga gtaaaagatg actttatcac
     6781 atatggaggg aatcatatag gagtgtattt tcttgaaaat gggtatggag aaagaccttc
     6841 tgaagtgact tatttgaata ggttaaatag ttcattttgt gagagtaaat tacaagatta
     6901 tgatattgat aaatgtttag aagaaatgga tatagtcctt atttgtggta ttgctatgca
     6961 acttacagat tcaattaaag agattgcttt agaactagct agaaaatctt atgaaaaggg
     7021 gataaaagta ttctttgatt ttaattttag acaatcatta aatccagaaa ggacttatga
     7081 tgatttagta aatgattata agaaaatact accatattgt catggtctat ttgctagtta
     7141 tagagatata aataagatac ttaatataga tagtctaaat ataaaatcta gttcagaatt
     7201 gattaaaaaa gagtatgata taggtattgt agctggaact atgagaagat ttgatgatat
     7261 gaataataga tttataaagg gatatatatc aaatgatgat ggttattttg aaagtaggga
     7321 gtataagtta gaaatatatg acagggttgg tgccggagat ggatatgtag caggaattat
     7381 atattcatat ttggaaaata tgaagagtga agatatagtt gagtttgggg ttgctagttc
     7441 ggtgttagct catacaactt atggggataa ttctttagta agcacaggtc atgtacttag
     7501 acttatgaac aatgaaaaaa tagatatatt gagataatac atttgtatat aataaaattt
     7561 ttatcttttt tagacacact tttgacttgt ttatatttgt taaacagagt tatttttaat
     7621 gattataaat attaaattaa aagttaaata ataaaatagt agaaaatagc attaactata
     7681 gaaaaaatgg ttaatgctat ttttatcgtt aatattgatt aattttatat tatagaaatg
     7741 aacttttcta atgctggatt tgtatttttt ttattccata caactacaac ttcaattcta
     7801 ttttctactt cttttatagg tactattgca atatcacgtt ctatcattgt atctgtaaca
     7861 caaaatgaag gtaaaatgga tattcccatg ttagaagata ccaatgattc aatagtatta
     7921 agattatttg aacgttgtac cacattaggt acaaaccctg cttcacgata tctattctgt
     7981 atcattgact tttgtccata ctcatcccct gtttctttta caaaaataaa tttatcatgt
     8041 tgtaattcaa ttaaatcgat agaatcctca ttgtaaagtg gatgaccagg aggtaaaaga
     8101 gctaccaaag aatatctttc tacagtttta tattcaaaat cttttaatgg ttgattttct
     8161 ggattaataa caaaagcgag gtccatagaa ttatttttta ctaagtttaa aagttcggtt
     8221 atttcattag tacaaatatc catagatata ttagggtatg tattagaaaa ttcttttaaa
     8281 tattgttgaa gtttgttttt ttcatatcct attaaggttc caatgctcaa agttccaaaa
     8341 aaaccatgtg caaattgttg tgtttttgca atagcatcat tgatattttt tattatagta
     8401 cgtgcctcag ataaaaaaac ttttccagct ggtgttagtt caacatgacg tttacttctt
     8461 gtaaatagag ttacatttat ttgttcttct aaagctttta tttgctgtgt aacagcagtt
     8521 tgagaaatat aaaatttttc tgctgttttg gtaaaactta aattttctgc tacagatata
     8581 aaatattcta attgtttagt attcaaataa tcacccctat ttaaacttga ttgtttgtaa
     8641 accttattta tctataatta aagcttatta tagtataaga aaatttactt ttaaagaata
     8701 ttagaaaata tttgtataaa aatatatgtt ttaagtatat ctttacatta gattttataa
     8761 atttattaat aagaataact tatatattcc aatgaaaacg atattggatt taagtaagtt
     8821 tgtctcataa aatgaaagta tatcaattga taatacttta aataagaagg gagaaaaaat
     8881 ggaagataaa ttttatgcaa aaggcaacgg aaataacgga tatattaaaa atcttgaagt
     8941 ttgttccttt aataacttag atggaacttg tggaatgttt caaatggctc tgtacaaaag
     9001 agatgaaaaa tactatttat atggatgctg ttttggagga aataaaaaaa atggagtaat
     9061 gattagcgat attacagacc cttataatcc acaatttata aaacattttc aaatgttaga
     9121 ccctaaagag tatcctacaa caacaactcc caaaattcaa atagcagatg atttaatgat
     9181 agtagcaatg agttgtggaa gtggaccagg agcacttgtt gaccaagcta aattagcaaa
     9241 tattaagtgt gaagcaggaa ttagaatata tagtttaaaa gaagaccctt taaatcctaa
     9301 gtttttagga tattgggatt gtggcttaaa gcatgtaatg ggtgttcata gattcatgta
     9361 caatggtgga agatatgtac atttatcaag tgattgtgtt ggctttgaag gtctgattta
     9421 tagggtcata gatataataa atcctactaa tccagtggaa ataggtaaat ggtggagacc
     9481 agaccaatat gcagatggat atccaaatag aacttttgat gcaggagcac ctcattgccc
     9541 agaatttatg gataaaggat ggcttcatgg acctccattt gtaagagacg gaaaagcata
     9601 ttgtggttat ggaggagctg gtttagttgt attagatgtt gaagatttaa caagaccaag
     9661 atgcttaggt gaattgccat ttatgcctgc attttctagt agacttgcag gtgcaagaac
     9721 tcatacagca ttaccattgc caggaagaga tttagtcgtt gttcaaaatg agggagaaag
     9781 attccagttc tttaaaccag ataacattac agatgttcaa gctatgaata atatacatat
     9841 ggttgatgtt agtgacccaa caaaaccaac attaattgct caatttccat atcctgaagt
     9901 tccaaaagat ttcccttatc ctaactttaa tgttgcagga ttaggaaaac cagggccatt
     9961 tggtccacat aatcttcatg aaccaatgga taataaacca tggttagagc aaagaggaga
    10021 tagagtatat tgctgttatt tccatgcagg gctaagggtt tatgatgtat cagacccata
    10081 ttatatcaaa gagctagcat attttatacc accaaatcca aataaaacac cagaagaatc
    10141 ttatttccca ggattcccag gaccacgctt ggcagtaaca gaagatctta tcgttgatga
    10201 tagaggctac atcatcatag atgctttaga tgatggattc tatatattaa aaatgaaaga
    10261 agattaaata aatactaaag agggagagtt gaaaatgaaa atactaggta tttcttttgg
    10321 aacaaaaaat ggaaataatg acacaatgtg taaagttgca cttaaaggtg ccaaagaagc
    10381 aggggctgaa gtagaattta ttcaaatgtc atctattaac attaaacatt gtactggatg
    10441 ttgtgcgtgt gttaaaactt tattatctgg gaaaggtagt atgtgtgtat taaaagatga
    10501 ttttgaatgg ttgcttgaca aaatgaaaga tgcagatggt atagtagttt cagaccctat
    10561 ttttgaagaa ggggcatcag gattattcca tactataatg gaccgttttg gacccagagc
    10621 agatagagga aataacatta taggaacaaa agttgcagaa gctataggag gaaaaattcc
    10681 agattcaaga atgcttaatg ataaagtaat atcatttatg ggaataggag gttctgactg
    10741 gggtacaaga gttcaatgtg aacatgctat gctagctctc ataccaatgt ggaaagttat
    10801 agataatgct tggttcccat gggcaaagga attagtaatg gatgatacaa ggcttgttca
    10861 agttcatcaa attggtttaa atatagttga agcagcaaaa gattttgaaa aagcttcata
    10921 tcaaggagaa gaaggtgtat gtccacattg ccacaataga ttattctacc ttgagccagg
    10981 tactaaaaag gcaatttgtg ctctatgtgg tatagttgga gagttagata ctataagtgg
    11041 aaaaactata tttagttttc cagaagagca attaggtcat gctcatgata ctttaccagg
    11101 taagtttata catggtgatg atattaagaa aatggaaggt cattatgcag aagttcgtaa
    11161 aacacaagaa tttaaagaaa gaaaaacatc ttataatttt atagagacat taactaaaga
    11221 aaaacaagct ggagtttcat taggataata attaaaatac taagactctt ttttaaggga
    11281 tgttagatgt tataggaggc ttattgtatg aatgaaaagc caagttttaa acaaattgaa
    11341 gttattttag cagttatttt tttggtactt acatattaca aggttagatt aactccagca
    11401 tttattgtta tatttatacc actatattta agttattttg cagtatctat gattgtaaat
    11461 caaatagcaa ttaagctatt ggagataagg caagctaggg aagctactaa ttacaatata
    11521 ggaaatggat agaaagaaaa aagaaatatt aacactgtct atggggattg ctttactacc
    11581 tcctttatgg gcagtattag caccttatat aggaataaaa actggagctg tagcactaat
    11641 ctgtgcagga ttatatgtaa ctaatggtaa taaacaagaa gatggattaa aaataatgtt
    11701 tggtttttgg tgtggagatt tatgggcagt gttagccatt ttaattatgg gatatatgaa
    11761 tttcaatcaa aacctagaat tatttttaac acttagtata ttaggttttt ttgcagttgt
    11821 aattgcatcc ttatttgaaa aaataatttt cctaccatca tggctttgtg gttgggctat
    11881 aggtctaact attatgacag gtgaaaatat tattaattta caagatattt gtattcaaat
    11941 tgctgtagct atggcagtag gagtatggta cgtgggtgca ggtgtagatt tgtttacaat
    12001 atatattttg agtaaagata ggaaaaaggg aaattgaaag catgtgaata tactttgatt
    12061 ataggaggat ggctatgggt tttgaattta aaataatgag aagtttaata tatgtaggac
    12121 ttgccaagga agaatataga cctaagctaa tggactggtt atatcgtcac catattccag
    12181 atagtattag cacttttgga ccatattgta ctaaatatgc cttttatcaa gcatatccta
    12241 caccaaatga aggtgagcgt tttggtgcac gtaagatgca actaacagaa cattattggc
    12301 ttgtagatga acatatgcct gagatggcaa atagaattat gacagaatat atgcctatgg
    12361 atgttctacg ttggcaaggg tgtataccag atgtagaaaa taaaagggtt catgaaaatg
    12421 cagaaagtgg agatgcagga cgtgcagtag gtggagataa tggatgtcca ccatttatat
    12481 ttgcctttgt tccaataaac tgggaagaag actttagagg aaaaggacgt actgtacaag
    12541 atggaccaaa ctatcgttgg caatttatga ttaagtatcc agatggtatc tctaaagaag
    12601 aaggagaaaa atggttctat gatgaggtag tgccatactt tacaaactgt tgctatgtta
    12661 atcgttttgt cagtagtaaa ataatgatta attatggagc aactgctttt gaccgtgtgt
    12721 cagaactatg gtttgaaggg gaagaagaat ggtataaagc tgtggttgaa gagacaaagt
    12781 cgtttattaa aaaaccagaa tgggcacaag aagaggagtt cccatattta aaaccacaat
    12841 tcaatatcgc atcagtattc ttaggtgata tagcaactat ggatgcatac tcacagtatc
    12901 gtggatatat accaatgaga taaaaattat agtttgtgat aagtagagaa ggcatttact
    12961 cttactggta gaagaagact tattgtatcc ttaaaagtag gtgaatatat agttttatat
    13021 ttaaatttat ataaaactaa gactaataaa tatgaaacaa tagaattaga ataatttgat
    13081 aatatttgtt aaaagagcat ttagaatgtg tttaaagatt aagatattcc ttttatggta
    13141 gcagatagtt gaaataataa aactgttatc atgaaaggaa tatttttatg tataggaaat
    13201 ttttaaaaat aaaagttaaa tatgtttaag aacatcttaa agtgaaagaa tcattgttag
    13261 aaatatgtag acaatatagt atttataacg tatcatatta acaagtttac acttggatta
    13321 tgaaataaaa agagtaattc taaaggttgt aaatactatg ctgactttga tacttcaaag
    13381 tttaggtgat tttgtaattg ttaatagaaa tacgtttaat gagcataaag aatttatcta
    13441 ataataggag aatataatgt atacaattgg acaagtatct aaatttttag gaatatctag
    13501 agatacacta aaattttatg aagacaaagg attagtaaat cctaagaaaa atgatgaaaa
    13561 tggatataga atatataatc aggttgatat ctatgatatt gcaacaataa acttttatag
    13621 agaaattgat atagaaatta aaaagattca agaaataaga aaaagtaaaa gtataaacaa
    13681 tttagaactc ttactagaag agaaggaaca aatcatacta aaagagatag aatataaaaa
    13741 gcttctctta aaaaaggttc agttagtcaa agaagattgt gacaaaatca aaacatattt
    13801 tggaaaatac acaataaaag aaatgaagcc tttggaagta aaaggtaaaa taaaacatgt
    13861 tactaactat gaggaatatg aagccttaaa agagaataca aacaacttaa aaaaagcagt
    13921 tacattaact gatttaagaa gagtaataaa ttttaatgaa gaaggtataa tagaagaaaa
    13981 atttataatt gtaagggaag tagatgacta caacaaaatc atagaaggtg aagttctatc
    14041 tcatcccaat tgtatttata ctattattga agatggcaga tggtcaactg gaggagaaaa
    14101 tattgataat aaagtggaag acagattaag agaaatagca atagaaaatg aatgtgaatt
    14161 acttggaata gtctatgtta atatattgct tactacttat gaagaaggga ttgagcgtat
    14221 atttttagag atttatgcac ctataaaata atataaaagc attgacctgg gacttactca
    14281 taggtttaaa ctcatgatat aagcagttat aacttatata gcaattaaat attaatttga
    14341 aatgaggatt acttatgaga acagagaaat cattaaaaag gcctatagcc agttttatta
    14401 ttctaacaaa tataattttt ttaccacttt ttttattggt agtagtgact ataatgttaa
    14461 aacttccaac agtgatatct gacattgcgt tgtgtatatc agcgtggtca tccacttttg
    14521 catttatgat attatttaaa aaaatttatc caggtaagaa atttttagta catgtaaaag
    14581 atagatttag aaataaactt aaattttcaa cagtatctat aataattagt atacaagtcc
    14641 taatagctgt agtagttata tttatattag catctaaaaa tcatggaata atgcctaact
    14701 ttacagtttc atcatgggga gcatttatat atttattttt aaaaaattta tttgcaggat
    14761 cattaggtga ggaattaggt tggagaggat ttgttcaaaa tcatcttcag aaaagacatt
    14821 ctcctttaaa agcatccata acagtaggtt tttggtgggg gatgtggcat ttaccaatat
    14881 ggtttacaac aggattcaaa ggacttgatt taataaaata tattatattt tttatgattg
    14941 caattatatc aatcagtatt ataatgacaa ctttttataa tctaaataaa actttgataa
    15001 tacctattgc aatacatcaa ctatttaatt ttttaatagg gataattaat gaagatttaa
    15061 tagagcttat aaattactat gcaatgttat atttaatatt tgcaattgca ttaatagtta
    15121 taaatccagg aaatgtatta tataaaaata ttagagagta atatatatct ttatggtaag
    15181 atataaagat attattagaa gaacatccaa ggaggaataa acatggtttt actgattgtt
    15241 gatgcacaaa agttaataac aaacgagagg ttatataaat ttaatgaatt tgtagtaaat
    15301 gtagaaaatt taattgatac agctagaaaa aataatatag aagtaatata tgtacgtcat
    15361 gatgatggag ttgaaaatga attgactaaa ggtaaaaatg gctttgaaat atacgaaaaa
    15421 tttaaaccat gtaatggaga aaaaatattt gataaaaaag taaatagtgc ttttaaagaa
    15481 actggtctat tagaatacct aacaaataat ggtgaaaaag atattattat tgctggtctc
    15541 caaacagatt attgtattga tgccactata aaatgtggat ttgaacatgg atttaatata
    15601 atagttccag cttattcaaa tacaactgtt gacaattttt ttatgtcagc agaacaatca
    15661 tataaatact acaatgaatt tatatggaat gaaagatatg cgaagtgtat ttcattagag
    15721 gaaacactta gaaaaatgaa ataaaaatag agtatgaatt gtatatgtac ataagttgat
    15781 ttcataaaaa aggatgttaa attccaagtt aaaaattaga ttaaatatat tatattatag
    15841 ataaggcaca tcaattttat tttaaattaa tgtgcttttt aattgtagta atttatttat
    15901 aatcaatatg atttttatta tttaaatcaa tttttcttga gtttctttgt ctaaagaaac
    15961 tttttttatt tataaaatct acagttatat tatagtatct attttagatg agtttaattt
    16021 ttgtatcaat ctaaaacaaa agttatactt aaatgaaatt gaaaacgata aaattctcaa
    16081 ataaaataag tattgtggag gtgatgaaag aatccaatga taatatgaag actaatctta
    16141 ataagaaagt aggggttaat ggtagtggac aaagaaattg tatcaattct ccagatttta
    16201 ttaaatagca cattgattac aactaatgga ttacaagagg agtctaattc atcaaagaga
    16261 caaattactt atagaataaa taaaatcaat gacatgctta aagtaaaaaa tgtaccactt
    16321 atatgtttaa gagccgacaa agatattatc gttagaaaag aaacaaaaaa agcaattaaa
    16381 gaaattttag aaaaaaacta ttctaagaat acttattatt ttagtaagac agaaagactt
    16441 ttatatatgt atttaatgct ttttattaac atggaggata tttcaattaa tcattttata
    16501 aattctataa aagtaagtag gtctactgta aatcttgatt tcaaggattt gataccagag
    16561 cttgaaaaga agaacattaa agttaagaac aatcgtataa atggatatta catagttggc
    16621 aatgagatgg aaattaggag agtacttatt aaaaacatta tagaaacttt gtctaatgag
    16681 aaaagttcaa gagtatttga tatttttatt aaagaatata atttagatag ttttgaagag
    16741 gctaaaaaaa ttatattaaa gctaataaaa aaatatgaaa ttacttttgt tgaagataga
    16801 ttaatagaat ttatatatat atttatcttt ctaaaagcaa ggatgtattc aactagaata
    16861 gttactcagg aaaatgtaga tattcctaat atttcagtta tgagttcaat gaaggagtat
    16921 aaatttgcta aggaattatt agaagtatat gggagtgaag ataagataaa gcctcacaat
    16981 ataatgtata ttagtgcttg gattttaggt atatcagtag gaaattatga agaagataca
    17041 gaagataagg atgtaatttc aaaaattgta aataaaatga tgacaaagtt tggatattta
    17101 agcggagttt actatataag ccaagaaaaa atatttaaac aattatattc tcattttaga
    17161 cctgcctact atagactttt atttaaatta cctatctata atccgatttg tgaaaaagta
    17221 aaagaagaat ataaattagt ttatagaatt gtaagtaatg caatgaaaga gttttgcgga
    17281 ttatttggag aagaaattca ggaagaagag ttagcatatc ttacaatgca ctttgcaaca
    17341 cttttttcaa ataaaaaaga atttgatggt tttagaaaaa gaatagcatt aatagtttgt
    17401 tcaaatggcg ttggtagttc agctatttta catgcagaac ttacaaactt atttcctgac
    17461 ttacacttta taccaatgtc ggaatcagtt ggattagaaa atataagtga acctgtagat
    17521 attgtattta ctactaatta taacgcagag ggtttaaaaa cagatgtacc agtaattaaa
    17581 gtaagccctg ttatgaccac aaaagaaaaa tataagatta cacgtgaagt gtatattcaa
    17641 ttaggtgata tttttttaag acaacctaaa atagatgagg ttatggatat aataaaaaaa
    17701 tatgcaaatg taacctctga aagtattctt tcaagtgagc tattagctta ttttacacag
    17761 atagagaatt ttacatctaa ggaaggagag gggcctatgc taagtgagat tactaatgaa
    17821 acactagtaa aaataaaggt aaaagctaag aattgggagg aagctattag acagtcagct
    17881 tctgtattag ttgaaaataa caaagtaact gaagggtatg tagatgctat gattaactca
    17941 gcaaaagcat ctggaccata catagtaatt acaaaacatg ttgcacttcc acatgctaga
    18001 ccagaagctg ggtcaaaaga gattgcaata ggcattgcta ctttagatag tcctattgag
    18061 tttgggaata acgaaaacga ccctgtaaaa tatgtatttt gtctaagtgc aatagacaat
    18121 aatagccatt tacgtgctat gtctgagcta gttgaactat tggaagaaga tgagtttttt
    18181 aaggttttag atagtgctaa agaagctaaa gaaattattg attttataaa aacacacgaa
    18241 ttataaagga ggatttaatt atgtataaaa ttttaatcgc atgtagagca ggagttggct
    18301 caagtttaat gttaaaaata aagactcaac aaattattaa ggaaaataac tttccaattg
    18361 aagtagagca tggttcattg gattcactaa atggattcaa tggagatgct gtagaaacat
    18421 taattgatgt tgctgaagaa ttaaaaaata aaaatttgaa gtttgatata attgggattc
    18481 acaatattgt tgaccgtaat gaaataaaga cagcactaga aaaattcctt gcttcaaaag
    18541 agggataaat ttaagatgat aaggaggaat atttatggag tttattatta gtttgctgag
    18601 tacacctgct gttttgcttg gcgtagttgc atgtatagga ttgattcttc aaaagaaatc
    18661 agctgttgaa gtatttacag gctcttcaaa aacacttatc ggttttttga tatttggtat
    18721 tggtgcctct gctatgacag gagcacttca aaatttcaat aaactgttcc aacatggctt
    18781 tggaattact ggtgttatag cttcaccaga agcagctaca gcacttgcac aaggtacata
    18841 tggttttgca gtttcatgta cattaattct tggattcata ttaaatttag tatttgcacg
    18901 tattacaaag tttaagaata ttttctttac aacaggtcat agtttgttct tttcttgtgt
    18961 attagtttta attatgaaag cacatggatt tgataataca atcactattt taataggtgg
    19021 aactatctta gggtttatgt ctgctgcact acctcaattc tgtcaaccat ttatgcgtga
    19081 attaacaggt ggcgacgaac aagctattgg tcactttaac atgattggtt atggtttatc
    19141 aggttatatt gggcgtttat tttcaaaaca taaagatgat acaacagaga ctatagaatt
    19201 tcctaagtgg ttatcaatat tcagagactt cttaatgggt ctatccatag ttatgttaat
    19261 cttattttat attgcaacat taaaagctgg tcaagcattt actcaagaaa ttgctggttc
    19321 aacacattgg ttggtttttc cgcttataga ggcatttaca tttgtggcag gtatgtcaat
    19381 attgatgtca ggtgttcgta tgttcttagc tgaaataact gcagcatttg taatcatttc
    19441 agaaaagtat attccaggtt cacgtcctgc gctagatgtt ccaacagtat ttccatatgc
    19501 accaaatgca gttattattg ggtttatatc agcatatgct gctggtctat tagcaattgc
    19561 tattatggca tcatttccta gtatatttcc tgtagtaatt attccagctg ctcacatatg
    19621 tttcttctca ggaggtacag ctgcaatatt tggcaatact tcaggaggat ggagaggggc
    19681 tattgctggg tcatttgttg tggggttatt acttgcattt ttaccagtta tactatatcc
    19741 agtttatggc actcttggta tagaaggagc aactttccct aatattgatt acaatgtagt
    19801 tggttcgata ttacataata ttttacaatt tataaaacct atttaaacta aattatataa
    19861 aagtatagtc atagttacta tgatttcaag gaggatacat cttatggaaa atacattatc
    19921 tgaattttta gataatatat ctaatgaatt gtcagaattt attgcctcaa tagatgagac
    19981 atctataaat aaagcatgcg aattaattct tgaagctgaa aaaaatcacg gacgtgtaca
    20041 tgtgactggt attggcaaac caggacatgt atcgggatat atttcatcat tgctttcatc
    20101 tactggaacg tcagcatata tattgcatgg tacagaagca gtacatgggt caagtggtca
    20161 agtagttgaa ggagatgttg ttattgcaat atcaaatagt ggtgaaactc aagagttaaa
    20221 agcaactctt aagactctaa aagtaaatgg agcaaagata attggtgtaa gtggtaatga
    20281 gagttcattt ttaaaaaata tttctgatat attcctgttt gcaggtgtta aacaggaagg
    20341 agattgtctt aataaagctc ctcgtgcttc aattttagct gaaacaataa tattacaatc
    20401 attaagtgtt gtattacaat atgctaaagg tcttaataca cagcagtatt tgaagtggca
    20461 tccagcagga agtcttggaa agtctattag agagggaata taattttatg aaatataaag
    20521 ggattatatg tgcaatgata acaccatttg atgaaaatca aaatattaat tcacaagcaa
    20581 catgtcaatt aattgattat ttgattgaaa agggaattta tggattattt attttgggca
    20641 ctaatggtga atgtcatgta ttaacagatg atgaaaaagt tgaatttgca aagattgtta
    20701 ttaatcatac aaataatcgt gtacctgttt ttgttggaac agggggtaat tcaactcgtg
    20761 aagttgtaga tttatctaag agaatggaag cgattggagc aagtgcatta tctattatta
    20821 caccatactt tgttacacca acacaacagg aattaatctt acattacaaa actattgcta
    20881 attcagttaa tttacctatt ataatgtata atatgccaag taagactgga attaatattg
    20941 aaccagagtc agtacgtgaa ttatcaaaga taaagaatat tgttggaatt aaggatagta
    21001 gtggtaaact tgacaacatg aaagcttata ttgaagcaac gaagaatgag aattttagtg
    21061 tgttttcagg ctcagattca cttattctgg atagtctaaa agctggtagt caaggagctg
    21121 ttgcagcaac aacaaacttc ctcacagaga ttggtatctc tatctatgaa aactttatga
    21181 aaggggattt ggaagaagct caaaaagctc aaagtagtat agaagaactt cgacgtatac
    21241 tgaaacttgg aacaattcca tctgttatga agaaaactat tgtcttgaat ggaattaatg
    21301 ttggaactgc acgacttcct gtaactgaac caactgggga agtacttgaa gaaattaaac
    21361 gagtagttga aaattatagg acaatactta ataaaagtag tgagaataga taatataaat
    21421 agggaaatta cactggtata taattaatat atatattata aaacatgact aaaattaatg
    21481 aataagtaaa gtggtttgat gagtatagag gtgataatat gaaaaagatg aatataacca
    21541 aaagaatgtc agaatgtggg gctcttgcaa ttgtaagaga agaaaatctt aaccgtgcat
    21601 gtgaaattgc tgaaggatgt ataaaaggtg gtataacagt tatagaaatg agttataccc
    21661 taaataatgc tggagaaata attcaagggc ttaataaaaa gtatggtgaa acactttgtg
    21721 tgggagcagg aacagttttg gatagtgaaa cagctcgtca tgctatatta catggagctc
    21781 aatttattat tgcaccaaat tacaatgaag gtgtagcgaa gatttgcaat agatatcaga
    21841 ttccttatgc tccaggatgt acatcattga ctgaggcagt agacgcacta agtttaggag
    21901 caacttttat aaaagctttc ccaatatcag atttctatgg acctaaacta gtaaaggtat
    21961 ttaagacacc aatacctgat atgccagttt tagcaagtgg agggattaat attgaaaacc
    22021 tacatatatg gttagaaaat ggagttgatg tatgtggatt tggtggttta ttaactaaag
    22081 gttctgttga agatattgct aaaaatgcta aaaagattcg tgaaatcatt aaaaatataa
    22141 gaaaaattta acatagggct gtatcatagt atcttataat tactatgata cagccttttt
    22201 aatattattc aaattaaaaa cagtttacat ttctattata ttacatataa tttcagtaat
    22261 tttatcttaa tttttctatc tggaaaaaaa tgatttaaaa cttaagttat gatataattt
    22321 cattaaaaga aggcgatttt tagtaaatat gagaaaataa tgtgttttta ggggaaaagt
    22381 taaatacaga tttaagtgaa caagaatcga taattaagat attaaatggt gaatagggca
    22441 tcaaaaaaat tactaaatta aaagttttag agggagaatc ttagcaagtg tgtgtgaaaa
    22501 ttcaatttca aacaaaaaat aattgtaaat gaaatcaatt tatacacgtt gaagattcac
    22561 ttttatcatt atattaaaaa gcagagtgaa ttaaaagtga gtctaaagat aggaggataa
    22621 ttaaaacaat gaaagaagac attaggttac atcgaatgtg taaaaaagaa caagcaacaa
    22681 agaatgaagt tatatcagag gatattctaa aatctatgag tattggggtc agtaaacatt
    22741 ttttagatga ttcatttaca ataatatggg gaaatacatc attctatcaa ttattaggat
    22801 atacagaaga agaattttta gttaaatttt ctagtttaaa agattattat gaacagaatc
    22861 tttatcattt tgaatctatg aaaaatcatt ttttagaggc ttatcatcat ggcgaaaaga
    22921 gtatagaata tcaaatttgc atttcagcaa ataatggaga ccctgtttgg gtaataatga
    22981 ctggtatatt aattgaatca caagataatg agaaatcaat ggtctatata ttgtattcaa
    23041 aaattaatga gttaaaaata aagcaagaag aactatctga tatggaaaaa gagagtacag
    23101 agaattttaa atggatgatg tcagagtatg ctgggaatgt ttatattagc gatatggata
    23161 catatgaatt actatactta aataagcact catgcaatac tttacaggca tcagcaaatg
    23221 aacttattgg aagaaaatgt tatgaggtaa ttcaaggtag aacttcgcca tgtccgtttt
    23281 gtacaaattc gtatttaaaa aaagatgaca tatatgaatg ggaattttat aataaaaaac
    23341 tgaaacgaac atttataatt aaagaccgta tgttgaattg gcaagggcga agagttcgta
    23401 ttgaattatc ttatgatatg tatagtgctg aatataaatt agcaaaaaaa gatcaggaaa
    23461 gagaatcaat tttgaaaact attccagcag gaatggttag attagatgca agagattatc
    23521 ttactatact ttggtgtaat gatatatttt tgaatatgat tgaatataca aaggaacagt
    23581 ttgcagagga acttaataat caatgtggat atttattttc tgaagattat aaacgtagta
    23641 agaaactggc acaagatttg aaggagtctg gggataatgt tgtatttgaa gcaaaaatat
    23701 atacacgttc aagggaagaa cgtatatgga ctgttacatt gtgttatatc agtggggaag
    23761 acagttggga tggaatacca tctttttata gtataggtat tgacataaca aaggaaagaa
    23821 aacagattga gaaattacag catatatcag agaaagatgc attaacagga atttataatc
    23881 gtgctgagac tgaaaggcag attaagaaat attttgaaaa aaatttaaat gttatgggtg
    23941 cactgtttat gattgataca gataatttta aacagattaa tgatacagaa gggcatatga
    24001 ttggagatat agtacttact gagatggcat caggtatgaa aaaaataatg cgtgacagtg
    24061 atgttgttgg aagaataggt ggagatgagt ttacaatttt tatgaagaat atttcctcag
    24121 ttaaagatgc agaaaagaaa gcagaagaac tattgtatat gttccgtcat ctatttcaga
    24181 aagaaaaaag ttttctaaaa gtaacttgta gcataggaat agctatatat ccaaaggatg
    24241 gtactacatt caaggaaata tatgcaagag ctgataaagc cctgtatcaa gcaaaaaata
    24301 tgggaaaaaa taattatgta atctacaacc aagattattt taaagaatta gaagaatttg
    24361 attgttcttc tcttggtaca gttattgatt cagaaaaaca atacgctgaa tatcctgata
    24421 atcttacacg ttatatattc cgtatgcttt atcagactga tgatatagac caggcgatta
    24481 atatgatact tgaggttgtt gggaaacagt ttgatgtaag tcgtgtttat atttttgaaa
    24541 atacagaaga tggtagatat acttctaata cttatgaatg gtgtaatgaa ggaatctctt
    24601 ctcaaatggc ttatttgcaa aacagcaatt atcaagacta tgaggattat gaaaagcttt
    24661 ttgaggatga attagttttt tattgtcgag atatccatac attaagtcct aaactagaag
    24721 aattgttttc tagtcaaggt atccactcta cacttcaatg tgcatataaa gaagatagag
    24781 ttttcagtgg atttgttgga tttgatgaat gcacaggact acgtctatgg tcacaagagg
    24841 aaataagtac attatcattg atttctcaaa ttatatcaat gtttttacaa cgtaaaaaaa
    24901 ataataaact taatcaacag attcaacaat accagaatat tttagataat attgaccagt
    24961 gtatttgtgt gattaataaa gaaaataatt ttttattata tgtaaataaa aaatttaaaa
    25021 atacatattc taaattccaa attggacaac cttattatct tgattctata gatattcata
    25081 agatatccat tatatggaat aaaaaagaag catacctatg tactagcacg atttaatggt
    25141 aaagtaaagt ttatattgga cttaaattaa atttcgtttt aaataagata ttattttagt
    25201 tgcttactga aaagactaga atctatatta atgaattata ctaattttat accactatta
    25261 aaatattgtt tgctaaaaat aaaaaatagc cttaaaacag ttgattataa aaaattaact
    25321 gctttaaggc tatttttatc tttgtgacga aatagacact tgccatttag aatgtgacaa
    25381 tatagtagtt gtttagaaaa ggcacaaagc attatacttt aactaaagta aaatgtattt
    25441 tgcttttaga ataaaaacaa ttaggaggga gctataattg aaaaagataa tatatgtatt
    25501 atttacaatt gaactattaa tagtatcttt attaggtcta aatatagtta aagataatga
    25561 aattaataat attttatata acgagacaac aagtatatct gtaatgttta aagattataa
    25621 aaagttaaat aaaaattata gtaagtggat taaagatatt gcagatgaaa ataatgtgac
    25681 tatatcaaag tatgttttta ataataatga aaaattatct atatatacga ctgatacaag
    25741 tttgaataac aatataaagt taaaatcagg aaaatttcct aatactaatt caaatgaatt
    25801 tattagtaat ataaacacta ataatagtaa tcaagtaggt aaattctcaa ctatgagtaa
    25861 agacatatca attttaatta gggattttgg taaaataagt gaagttggag aatcaggaat
    25921 cttatatatt tcaactcaaa atgaagatac aataaataaa atattaagag aactaaatgt
    25981 agacagaagt atttttgtag caagattaca tgatagatat gttaactcaa atttatactt
    26041 aaatccttca ataattagag atttaatact aattatcctt tgtttcttgg ctacaataat
    26101 acattattca attagtgtga gtagagaggc ttctatttta agattaaatg gatactctaa
    26161 gctagatata attgctagag ttataaaaaa tatacttaga attatgattt tatcatctgt
    26221 cacagcatta ttaatatata taatctatat tttaaaatta aatataggtg ttagagcatg
    26281 cttatacttt gctatgtttt cgattatatg tattttgata aatattttga tatctatatt
    26341 aatagttggt tttaatagta gaagttctaa atatgtgcta tctctaaatg gaaaaaaatc
    26401 ttatggtttt gtaaatataa tgcatttcac attgaaaatt gtatttgtat catttcttat
    26461 attatccata aataactgta ttttaaatta taatatgcta caaactcagt taggaaatct
    26521 atctgagtgg gataaagcaa aaaatgttta taatcttact cttaaagata caggcgaaca
    26581 atctttagga aaggaagaag taattaaaaa tgccaagatt aaaggtttct ataaaaattt
    26641 ggtagaagaa aaagatgctt tcttaataga ttcaactaat tttgaaaaac ttgaagatgg
    26701 aagttacatg tatgaaataa attcaaaggg taaaaaccca gaagtaagtc aatatggtaa
    26761 aaatataaaa ataaataaaa actatcttaa agttaaccct atttactcaa atggcaaaga
    26821 aatatttaat ctaatcgatt atgacgataa gactataaat ttattagttc ctaaaaagtt
    26881 acaagtacat gaaaatgaga ttaaaaaaga gtttagagaa ttattttatt ttgaaaaaat
    26941 agaagtagaa aatatgtaca ataaagagtt aaatagagcg ttaaacaaaa caaagaagtc
    27001 agatttaaat gttaatataa tatatgtaga taataatcaa agctatttta cttataactc
    27061 ccttgttatg gatgacaata ggaatctcat tgatgaccca atagcaatag ttgacataga
    27121 taatattgat gattcttttt atctttctta tatatctaga tgtgtatatt ttaattctaa
    27181 gaaattagat gcccttgcag atataagtag cattattgaa gctcaaggtg tagaagcgca
    27241 tatccaaagt ttacatgctg tttataatga gtatggttta gaaataaata aactagaaaa
    27301 atatttgaat agtgaaattt ttactataat aataatagca atatcaaact taatgattac
    27361 ctacaatata gtagctagct actatgaaag aaataaatat aagttatata ttaaaaaaat
    27421 atttggttat tcaactacag ctagaagtat gttgttaata gtctcattaa tattaacaaa
    27481 cattattcca ataggcatta tatcaacaag agtagattta agtaataata ttatcttatt
    27541 tggacttcta atattagtta tagaagtaat tgtatctatt gtattagaca agattatctc
    27601 aaatagttca tttaataaaa ttataaaagg agaacattaa aaagatgatt tcattaataa
    27661 atatatctaa aaaatttaaa gataaatcta taatagacaa atttaaccta gaaatttcta
    27721 agggcgagtt tgtagcgata acaggaaata gtggttcagg taagacaact ttacttaata
    27781 taatgggatt attagaaaaa cctaattctg gtgatattat aataaataat ataaaaaatc
    27841 caaatagtaa acagataaga attttacaaa gggattttta tggctatcta tttcaaaatt
    27901 atgctttaat tgagaatgaa aatgttgaaa ataacttgaa aattgcttta aagtatcaaa
    27961 aaaatatcaa ccataataaa aaaataaatt acgcacttga gtctgtaggt cttagaaaat
    28021 acaataaaaa aaagatatac gaattgagtg gaggagaaca acaaagggta gcattagcaa
    28081 gagtaatatt aaaagaatgt gaagtaatat ttgcagatga acctacaggt aatttagata
    28141 aaaaaaatag agacatagta tttgatatac ttaaaagtct aaataagaat ggtaaaacta
    28201 tcgtatttgt aactcatgat ttagagttat cggagcaggc agatagagtt ataaaattgt
    28261 aataatatca actataataa atataagaaa gagattacag ctaaatcaag tttaataata
    28321 atacgaatag aatttaaaca ttaagtaaat tagtagtttg taaatataag tataatatga
    28381 tatcataaag gctcaaaaga aattttatat aataagaaat ctaaaaataa caaatggagg
    28441 acatataaag atggaaaatt taccaaattg cccgaaatgt aattcagaat atacttatga
    28501 agatggaact cttttagttt gtccagaatg ctctcatgag tgggcattag gattgggagc
    28561 tgaagaagat tcaaatatta tcaaagatgt aaatggaaat gtattaaatg atggagattc
    28621 tgttacagta attagagacc ttaaagtaaa aggaagttca tcatctataa agattggaac
    28681 aaaagtgaaa aatatccgtt taatccatga ctcatcagat ggacatgata tagaatgtaa
    28741 gatagatggt tttggagcta tgaagctaaa gtcttctgtt gttaaaaaag cgtaatatta
    28801 taaataagct gtagtgttta ttgtttttca gtacagtata aaaccataag aatctattaa
    28861 gtaataaaag tttgtttatt tatctttata ttttcttatg gttttattaa atataaattt
    28921 tattgaacac caaaatattc tctcctctac aaataagcca atcttattta aattaatata
    28981 ctttgtacta tttatactga aattgtaaat ttaaatgcta tgttatttag gtttgtatag
    29041 ttaatttata tatttctgat tttataaatt
//
LOCUS       DAMJWS010000048        28851 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMJWS010000048 DAMJWS010000000
VERSION     DAMJWS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.048"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            104..658
                     /gene="tcdR"
                     /locus_tag="PC399_002212"
     CDS             104..658
                     /gene="tcdR"
                     /locus_tag="PC399_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF2593069.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            976..8076
                     /gene="tcdB"
                     /locus_tag="PC399_002213"
                     /gene_synonym="toxB"
     CDS             976..8076
                     /gene="tcdB"
                     /locus_tag="PC399_002213"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF2593070.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8198..8698
                     /gene="tcdE"
                     /locus_tag="PC399_002214"
     CDS             8198..8698
                     /gene="tcdE"
                     /locus_tag="PC399_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF2593071.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8777..8938
                     /locus_tag="PC399_002215"
     CDS             8777..8938
                     /locus_tag="PC399_002215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593072.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9027..9209
                     /locus_tag="PC399_002216"
     CDS             9027..9209
                     /locus_tag="PC399_002216"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593073.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9426..17558
                     /gene="tcdA"
                     /locus_tag="PC399_002217"
                     /gene_synonym="toxA"
     CDS             9426..17558
                     /gene="tcdA"
                     /locus_tag="PC399_002217"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF2593074.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17893..18591)
                     /gene="tcdC"
                     /locus_tag="PC399_002218"
     CDS             complement(17893..18591)
                     /gene="tcdC"
                     /locus_tag="PC399_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HDF2593075.1"
                     /translation="MFSKKNDGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            19017..19262
                     /locus_tag="PC399_002219"
     CDS             19017..19262
                     /locus_tag="PC399_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF2593076.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLI
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(19480..19749)
                     /locus_tag="PC399_002220"
     CDS             complement(19480..19749)
                     /locus_tag="PC399_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2593077.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(20627..21364)
                     /locus_tag="PC399_002221"
     CDS             complement(20627..21364)
                     /locus_tag="PC399_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593078.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            complement(21376..22137)
                     /locus_tag="PC399_002222"
     CDS             complement(21376..22137)
                     /locus_tag="PC399_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593079.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMFMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            complement(22137..23051)
                     /locus_tag="PC399_002223"
     CDS             complement(22137..23051)
                     /locus_tag="PC399_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593080.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QKLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            23222..23929
                     /locus_tag="PC399_002224"
     CDS             23222..23929
                     /locus_tag="PC399_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593081.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            23974..25380
                     /locus_tag="PC399_002225"
     CDS             23974..25380
                     /locus_tag="PC399_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2593082.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVEFDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            27179..27568
                     /locus_tag="PC399_002226"
     CDS             27179..27568
                     /locus_tag="PC399_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2593083.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            28302..28772
                     /locus_tag="PC399_002227"
     CDS             28302..28772
                     /locus_tag="PC399_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2593084.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
BASE COUNT        11439 a         3170 c         4458 g         9784 t
ORIGIN      
        1 ttaatttatt tgccgattat ataattataa tgactgattt aattccgatg ttgtcaaaat
       61 tttcaaataa atcatcatta taaatataag agaggatgat tttatgcaaa agtcttttta
      121 tgaattaatt gttttagcaa gaaataactc agtagatgat ttgcaagaaa ttttatttat
      181 gtttaagcca ttagtaaaaa agcttagtag agttttacat tatgaagagg gagaaacaga
      241 tttaataata ttttttattg aattaataaa aaatattaaa ttaagtagct tttcagaaaa
      301 aagcgatgct attatagtca aatatattca taaatcatta ctgaataaga cttttgagtt
      361 gtctagaaga tattctaaaa tgaagtttaa ttttgtagaa tttgatgaaa atatcttaaa
      421 tatgaaaaat aattatcaaa gtaagtctat ttttgaggaa gatatttgtt ttttcggata
      481 tattttgaaa gaattatctg gtattcaaag aaaagttatt ttttataaat atttaaaagg
      541 atattctgat agagaaatat cagtgaaatt aaaaatatct agacaagctg ttaataaggc
      601 taaaaataga gcatttaaaa aaataaaaaa agactatgaa aattatttta acttgtaatt
      661 aatgagctta aagaaatatt tacaatagaa atcaaatttt agaattaact ttattgtaaa
      721 atcaataact taatctaaga atatcttaat ttttatattt tatatagaac aaagtttaca
      781 tatttatttc agacaacgtc tttattcaat cgaagagcaa attaatcaac tgagtgtctt
      841 caatttaaaa tgttaggaag tgaatgtata tgaaaaccta agtagatatt agtatatttt
      901 ataaatagaa aggaggatat ataaaagagt tttatcattt agatgtaaaa atattatagt
      961 aaaggagaaa attttatgag tttagttaat agaaaacagt tagaaaaaat ggcaaatgta
     1021 agatttcgta ctcaagaaga tgaatatgtt gcaatattgg atgctttaga agaatatcat
     1081 aatatgtcag agaatactgt agtcgaaaaa tatttaaaat taaaagatat aaatagttta
     1141 acagatattt atatagatac atataaaaaa tctggtagaa ataaagcctt aaaaaaattt
     1201 aaggaatatc tagttacaga agtattagag ctaaagaata ataatttaac tccagttgag
     1261 aaaaatttac attttgtttg gattggaggt caaataaatg acactgctat taattatata
     1321 aatcaatgga aagatgtaaa tagtgattat aatgttaatg ttttttatga tagtaatgca
     1381 tttttgataa acacattgaa aaaaactgta gtagaatcag caataaatga tacacttgaa
     1441 tcatttagag aaaacttaaa tgaccctaga tttgactata ataaattctt cagaaaacgt
     1501 atggaaataa tttatgataa acagaaaaat ttcataaact actataaagc tcaaagagaa
     1561 gaaaatcctg aacttataat tgatgatatt gtaaagacat atctttcaaa tgagtattca
     1621 aaggagatag atgaacttaa tacctatatt gaagaatcct taaataaaat tacacagaat
     1681 agtggaaatg atgttagaaa ctttgaagaa tttaaaaatg gagagtcatt caacttatat
     1741 gaacaagagt tggtagaaag gtggaattta gctgctgctt ctgacatatt aagaatatct
     1801 gcattaaaag aaattggtgg tatgtattta gatgttgata tgttaccagg aatacaacca
     1861 gacttatttg agtctataga gaaacctagt tcagtaacag tggatttttg ggaaatgaca
     1921 aagttagaag ctataatgaa atacaaagaa tatataccag aatatacctc agaacatttt
     1981 gacatgttag acgaagaagt tcaaagtagt tttgaatctg ttctagcttc taagtcagat
     2041 aaatcagaaa tattctcatc acttggtgat atggaggcat caccactaga agttaaaatt
     2101 gcatttaata gtaagggtat tataaatcaa gggctaattt ctgtgaaaga ctcatattgt
     2161 agcaatttaa tagtaaaaca aatcgagaat agatataaaa tattgaataa tagtttaaat
     2221 ccagctatta gcgaggataa tgattttaat actacaacga atacctttat tgatagtata
     2281 atggctgaag ctaatgcaga taatggtaga tttatgatgg aactaggaaa gtatttaaga
     2341 gttggtttct tcccagatgt taaaactact attaacttaa gtggccctga agcatatgcg
     2401 gcagcttatc aagatttatt aatgtttaaa gaaggcagta tgaatatcca tttgatagaa
     2461 gctgatttaa gaaactttga aatctctaaa actaatattt ctcaatcaac tgaacaagaa
     2521 atggctagct tatggtcatt tgacgatgca agagctaaag ctcaatttga agaatataaa
     2581 aggaattatt ttgaaggttc tcttggtgaa gatgataatc ttgatttttc tcaaaatata
     2641 gtagttgaca aggagtatct tttagaaaaa atatcttcat tagcaagaag ttcagagaga
     2701 ggatatatac actatattgt tcagttacaa ggagataaaa ttagttatga agcagcatgt
     2761 aacttatttg caaagactcc ttatgatagt gtactatttc agaaaaatat agaagattca
     2821 gaaattgcat attattataa tcctggagat ggtgaaatac aagaaataga caagtataaa
     2881 attccaagta taatttctga tagacctaag attaaattaa catttattgg tcatggtaaa
     2941 gatgaattta atactgatat atttgcaggt tttgatgtag attcattatc cacagaaata
     3001 gaagcagcaa tagatttagc taaagaggat atttctccta agtcaataga aataaattta
     3061 ttaggatgta atatgtttag ctactctatc aacgtagagg agacttatcc tggaaaatta
     3121 ttacttaaag ttaaagataa aatatcagaa ttaatgccat ctataagtca agactctatt
     3181 atagtaagtg caaatcaata tgaagttaga ataaatagtg aaggaagaag agaattattg
     3241 gatcattctg gtgaatggat aaataaagaa gaaagtatta taaaggatat ttcatcaaaa
     3301 gaatatatat catttaatcc taaagaaaat aaaattacag taaaatctaa aaatttacct
     3361 gagctatcta cattattaca agaaattaga aataattcta attcaagtga tattgaacta
     3421 gaagaaaaag taatgttaac agaatgtgag ataaatgtta tttcaaatat agatacgcaa
     3481 attgttgagg aaaggattga agaagctaag aatttaactt ctgactctat taattatata
     3541 aaagatgaat ttaaactaat agaatctatt tctgatgcac tatgtgactt aaaacaacag
     3601 aatgaattag aagattctca ttttatatct tttgaggaca tatcagagac tgatgaggga
     3661 tttagtataa gatttattaa taaagaaact ggagaatcta tatttgtaga aactgaaaaa
     3721 acaatattct ctgaatatgc taatcatata actgaagaga tttctaagat aaaaggtact
     3781 atatttgata ctgtaaatgg taagttagta aaaaaagtaa atttagatac tacacacgaa
     3841 gtaaatactt taaatgctgc attttttata caatcattaa tagaatataa tagttctaaa
     3901 gaatctctta gtaatttaag tgtagcaatg aaagtccaag tttacgctca attatttagt
     3961 actggtttaa atactattac agatgcagcc aaagttgttg aattagtatc aactgcatta
     4021 gatgaaacta tagacttact tcctacatta tctgaaggat tacctataat tgcaactatt
     4081 atagatggtg taagtttagg tgcagcaatc aaagagctaa gtgaaacgag tgacccatta
     4141 ttaagacaag aaatagaagc taagataggt ataatggcag taaatttaac aacagctaca
     4201 actgcaatca ttacttcatc tttgggggta gctagtggat ttagtatact tttagttcct
     4261 ttagcaggaa tttcagcagg tataccaagc ttagtaaaca atgaacttgt acttcgagat
     4321 aaggcaacaa aggttgtaga ttattttaaa catgtttcat tagttgaaac tgaaggagta
     4381 tttactttat tagatgataa aataatgatg ccacaagatg atttagtgat atcagaaata
     4441 gattttaata ataattcaat agttttaggt aaatgtgaaa tctggagaat ggaaggtggt
     4501 tcaggtcata ctgtaactga tgatatagat cacttctttt cagcaccatc aataacatat
     4561 agagagccac acttatctat atatgacgta ttggaagtac aaaaagaaga acttgatttg
     4621 tcaaaagatt taatggtatt acctaatgct ccaaatagag tatttgcttg ggaaacagga
     4681 tggacaccag gtttaagaag cttagaaaat gatggcacaa aactgttaga ccgtataaga
     4741 gataactatg aaggtgagtt ttattggaga tattttgctt ttatagctga tgctttaata
     4801 acaacattaa aaccaagata tgaagatact aatataagaa taaatttaga tagtaatact
     4861 agaagtttta tagttccaat aataactaca gaatatataa gagaaaaatt atcatattct
     4921 ttctatggtt caggaggaac ttatgcattg tctctttctc aatataatat gggtataaat
     4981 atagaattaa gtgaaagtga tgtttggatt atagatgttg ataatgttgt gagagatgta
     5041 actatagaat ctgataaaat taaaaaaggt gatttaatag aaggtatttt atctacacta
     5101 agtattgaag agaataaaat tatcttaaat agccatgaga ttaatttttc tggtgaggta
     5161 aatggaagta atggatttgt ttctttaaca ttttcaattt tagaaggaat aaatgcaatt
     5221 atagaagttg atttattatc taaatcatat aaattactta tttctggcga attaaaaata
     5281 ttgatgttaa attcaaatca tattcaacag aaaatagatt atataggatt caatagcgaa
     5341 ttacagaaaa atataccata tagctttgta gatagtgaag gaaaagagaa tggttttatt
     5401 aatggttcaa caaaagaagg tttatttgta tctgaattac ctgatgtagt tcttataagt
     5461 aaggtttata tggatgatag taagccttca tttggatatt atagtaataa tttgaaagat
     5521 gtcaaagtta taactaaaga taatgttaat atattaacag gttattatct taaggatgat
     5581 ataaaaatct ctctttcttt gactctacaa gatgaaaaaa ctataaagtt aaatagtgtg
     5641 catttagatg aaagtggagt agctgagatt ttgaagttca tgaatagaaa aggtaataca
     5701 aatacttcag attctttaat gagcttttta gaaagtatga atataaaaag tattttcgtt
     5761 aatttcttac aatctaatat taagtttata ttagatgcta attttataat aagtggtact
     5821 acttctattg gccaatttga gtttatttgt gatgaaaatg ataatataca accatatttc
     5881 attaagttta atacactaga aactaattat actttatatg taggaaatag acaaaatatg
     5941 atagtggaac caaattatga tttagatgat tctggagata tatcttcaac tgttatcaat
     6001 ttctctcaaa agtatcttta tggaatagac agttgtgtta ataaagttgt aatttcacca
     6061 aatatttata cagatgaaat aaatataacg cctgtatatg aaacaaataa tacttatcca
     6121 gaagttattg tattagatgc aaattatata aatgaaaaaa taaatgttaa tatcaatgat
     6181 ctatctatac gatatgtatg gagtaatgat ggtaatgatt ttattcttat gtcaactagt
     6241 gaagaaaata aggtgtcaca agttaaaata agattcgtta atgtttttaa agataagact
     6301 ttggcaaata agctatcttt taactttagt gataaacaag atgtacctgt aagtgaaata
     6361 atcttatcat ttacaccttc atattatgag gatggattga ttggctatga tttgggtcta
     6421 gtttctttat ataatgagaa attttatatt aataactttg gaatgatggt atctggatta
     6481 atatatatta atgattcatt atattatttt aaaccaccag taaataattt gataactgga
     6541 tttgtgactg taggcgatga taaatactac tttaatccaa ttaatggtgg agctgcttca
     6601 attggagaga caataattga tgacaaaaat tattatttca accaaagtgg agtgttacaa
     6661 acaggtgtat ttagtacaga agatggattt aaatattttg ccccagctaa tacacttgat
     6721 gaaaacctag aaggagaagc aattgatttt actggaaaat taattattga cgaaaatatt
     6781 tattattttg atgataatta tagaggagct gtagaatgga aagaattaga tggtgaaatg
     6841 cactatttta gcccagaaac aggtaaagct tttaaaggtc taaatcaaat aggtgattat
     6901 aaatactatt tcaattctga tggagttatg caaaaaggat ttgttagtat aaatgataat
     6961 aaacactatt ttgatgattc tggtgttatg aaagtaggtt acactgaaat agatggcaag
     7021 catttctact ttgctgaaaa cggagaaatg caaataggag tatttaatac agaagatgga
     7081 tttaaatatt ttgctcatca taatgaagat ttaggaaatg aagaaggtga agaaatctca
     7141 tattctggta tattaaattt caataataaa atttactatt ttgatgattc atttacagct
     7201 gtagttggat ggaaagattt agaggatggt tcaaagtatt attttgatga agatacagca
     7261 gaagcatata taggtttgtc attaataaat gatggtcaat attattttaa tgatgatgga
     7321 attatgcaag ttggatttgt cactataaat gataaagtct tctacttctc tgactctgga
     7381 attatagaat ctggagtaca aaacatagat gacaattatt tctatataga tgataatggt
     7441 atagttcaaa ttggtgtatt tgatacttca gatggatata aatattttgc acctgctaat
     7501 actgtaaatg ataatattta cggacaagca gttgaatata gtggtttagt tagagttggg
     7561 gaagatgtat attattttgg agaaacatat acaattgaga ctggatggat atatgatatg
     7621 gaaaatgaaa gtgataaata ttatttcaat ccagaaacta aaaaagcatg caaaggtatt
     7681 aatttaattg atgatataaa atattatttt gatgagaagg gcataatgag aacgggtctt
     7741 atatcatttg aaaataataa ttattacttt aatgagaatg gtgaaatgca atttggttat
     7801 ataaatatag aagataagat gttctatttt ggtgaagatg gtgtcatgca gattggagta
     7861 tttaatacac cagatggatt taaatacttt gcacatcaaa atactttgga tgagaatttt
     7921 gagggagaat caataaacta tactggttgg ttagatttag atgaaaagag atattatttt
     7981 acagatgaat atattgcagc aactggttca gttattattg atggtgagga gtattatttt
     8041 gatcctgata cagctcaatt agtgattagt gaatagataa aaatatgtta aatatatcct
     8101 cttatactta aatatataaa aataaacaaa atgatacact acataaagtg ttctatctaa
     8161 tatgaagatt taccaataaa aaggtggact atgatgaatg cacagtagtt caccttttta
     8221 tatttctaat ggtaacaaaa tattttttta tataaaccta ggaggcgtta tgaatatgac
     8281 aatatctttt ttatcagagc atatatttat aaagttagta attttaacta tatcatttga
     8341 tacattatta ggatgtttaa gtgcaataaa aagtcgtaaa tttaattcta gttttggaat
     8401 agatggagga atcagaaaag tagcaatgat agcatgtata ttttttttat cagtagttga
     8461 cattcttaca aagtttaact ttttatttat gttaccacaa gattgtatca attttttaag
     8521 actaaaacat cttggaatat ctgaattttt ctctatttta tttattttat atgaaagtgt
     8581 aagtatatta aaaaatatgt gcttatgtgg attaccagta cctaagagat taaaggaaaa
     8641 aatagcaatt ttactagatg caatgacaga tgaaatgaat gctaaggatg aaaagtaagt
     8701 aatggtagat ataataaaga tattaacaaa taaaaagtgt tatccaaata agaatagctg
     8761 aaagttatca taattcatga aactaataat gaaaacgagg gagcagatgc caagagacac
     8821 acaagtatta aatacatata atttcgaagc aagtgttcat tactatatag atgacaaggt
     8881 agtatatcaa acattggttc acaaagatgg tgcatggtca gttggtaaaa tctattaagc
     8941 tacattagtt acagatatca caaactataa tagttaaaca tagaaatatg tgtaaattgt
     9001 gatggaaatt attcaaaaac acaaaaatac gtgatgaagg acaaaatgat atagaaaata
     9061 agtatcaaac cttaataaat gatttaattg atagtttaaa agttatagga aaaatatata
     9121 aagaaataaa aacattaaaa aaatataaga tatgtttaca aattactatc agacaatctc
     9181 cttatctaat agaagagtca attaactaat tgagtatctt taaattgaaa tgttaggaag
     9241 tgatttaaat atgaaaactt aaattataaa aaatcaatat taatttattt ttaaaaaata
     9301 gaaaggagtg tataagattt attttcaaag tttaaaaaca agaaaatcaa tttaaatttc
     9361 agaaggaata aatgtggtta tagaagtgga tttattatca aaaataataa tactaggagg
     9421 tttttatgtc tttaatatct aaagaagagt taataaaact cgcatatagc attagaccaa
     9481 gagaaaatga gtataaaact atactaacta atttagacga atataataag ttaactacaa
     9541 acaataatga aaataaatat ttacaattaa aaaaactaaa tgaatcaatt gatgttttta
     9601 tgaataaata taaaacttca agcagaaata gagcactctc taatctaaaa aaagatatat
     9661 taaaagaagt aattcttatt aaaaattcca atacaagccc tgtagaaaaa aatttacatt
     9721 ttgtatggat aggtggagaa gtcagtgata ttgctcttga atacataaaa caatgggctg
     9781 atattaatgc agaatataat attaaactgt ggtatgatag tgaagcattc ttagtaaata
     9841 cactaaaaaa ggctatagtt gaatcttcta ccactgaagc attacagcta ctagaggaag
     9901 agattcaaaa tcctcaattt gataatatga aattttacaa aaaaaggatg gaatttatat
     9961 atgatagaca aaaaaggttt ataaattatt ataaatctca aatcaataaa cctacagtac
    10021 ctacaataga tgatattata aagtctcatc tagtatctga atataataga gatgaaactg
    10081 tattagaatc atatagaaca aattctttga gaaaaataaa tagtaatcat ggtatagata
    10141 tcagggctaa tagtttgttt acagaacaag agttattaaa tatttatagt caggagttgt
    10201 taaatcgtgg aaatttagct gcagcatctg acatagtaag attattagcc ctaaaaaatt
    10261 ttggcggagt atatttagat gttgatatgc ttccaggtat tcactctgat ttatttaaaa
    10321 caatatctag acctagctct attggactag accgttggga aatgataaaa ttagaggcta
    10381 ttatgaagta taaaaaatat ataaataatt atacatcaga aaactttgat aaacttgatc
    10441 aacaattaaa agataatttt aaactcatta tagaaagtaa aagtgaaaaa tctgagatat
    10501 tttctaaatt agaaaattta aatgtatctg atcttgaaat taaaatagct ttcgctttag
    10561 gcagtgttat aaatcaagcc ttgatatcaa aacaaggttc atatcttact aacctagtaa
    10621 tagaacaagt aaaaaataga tatcaatttt taaaccaaca ccttaaccca gccatagagt
    10681 ctgataataa cttcacagat actactaaaa tttttcatga ttcattattt aattcagcta
    10741 ccgcagaaaa ctctatgttt ttaacaaaaa tagcaccata cttacaagta ggttttatgc
    10801 cagaagctcg ctccacaata agtttaagtg gtccaggagc ttatgcgtca gcttactatg
    10861 atttcataaa tttacaagaa aatactatag aaaaaacttt aaaagcatca gatttaatag
    10921 aatttaaatt cccagaaaat aatctatctc aattgacaga acaagaaata aatagtctat
    10981 ggagctttga tcaagcaagt gcaaaatatc aatttgagaa atatgtaaga gattatactg
    11041 gtggatctct ttctgaagac aatggggtag actttaataa aaatactgcc ctcgacaaaa
    11101 actatttatt aaataataaa attccatcaa acaatgtaga agaagctgga agtaaaaatt
    11161 atgttcatta tatcatacag ttacaaggag atgatataag ttatgaagca acatgcaatt
    11221 tattttctaa aaatcctaaa aatagtatta ttatacaacg aaatatgaat gaaagtgcaa
    11281 aaagctactt tttaagtgat gatggagaat ctattttaga attaaataaa tataggatac
    11341 ctgaaagatt aaaaaataag gaaaaagtaa aagtaacctt tattggacat ggtaaagatg
    11401 aattcaacac aagcgaattt gctagattaa gtgtagattc actttccaat gagataagtt
    11461 catttttaga taccataaaa ttagatatat cacctaaaaa tgtagaagta aacttacttg
    11521 gatgtaatat gtttagttat gattttaatg ttgaagaaac ttatcctggg aagttgctat
    11581 taagtattat ggacaaaatt acttccactt tacctgatgt aaataaaaat tctattacta
    11641 taggagcaaa tcaatatgaa gtaagaatta atagtgaggg aagaaaagaa cttctggctc
    11701 actcaggtaa atggataaat aaagaagaag ctattatgag cgatttatct agtaaagaat
    11761 acattttttt tgattctata gataataagc taaaagcaaa gtccaagaat attccaggat
    11821 tagcatcaat atcagaagat ataaaaacat tattacttga tgcaagtgtt agtcctgata
    11881 caaaatttat tttaaataat cttaagctta atattgaatc ttctattggt gattacattt
    11941 attatgaaaa attagagcct gttaaaaata taattcacaa ttctatagat gatttaatag
    12001 atgagttcaa tctacttgaa aatgtatctg atgaattata tgaattaaaa aaattaaata
    12061 atctagatga gaagtattta atatcttttg aagatatctc aaaaaataat tcaacttact
    12121 ctgtaagatt tattaacaaa agtaatggtg agtcagttta tgtagaaaca gaaaaagaaa
    12181 ttttttcaaa atatagcgaa catattacaa aagaaataag tactataaag aatagtataa
    12241 ttacagatgt taatggtaat ttattggata atatacagtt agatcatact tctcaagtta
    12301 atacattaaa cgcagcattc tttattcaat cattaataga ttatagtagc aataaagatg
    12361 tactgaatga tttaagtacc tcagttaagg ttcaacttta tgctcaacta tttagtacag
    12421 gtttaaatac tatatatgac tctatccaat tagtaaattt aatatcaaat gcagtaaatg
    12481 atactataaa tgtactacct acaataacag aggggatacc tattgtatct actatattag
    12541 acggaataaa cttaggtgca gcaattaagg aattactaga cgaacatgac ccattactaa
    12601 aaaaagaatt agaagctaag gtgggtgttt tagcaataaa tatgtcatta tctatagctg
    12661 caactgtagc ttcaattgtt ggaataggtg ctgaagttac tattttctta ttacctatag
    12721 ctggtatatc tgcaggaata ccttcattag ttaataatga attaatattg catgataagg
    12781 caacttcagt ggtaaactat tttaatcatt tgtctgaatc taaaaaatat ggccctctta
    12841 aaacagaaga tgataaaatt ttagttccta ttgatgattt agtaatatca gaaatagatt
    12901 ttaataataa ttcgataaaa ctaggaacat gtaatatatt agcaatggag gggggatcag
    12961 gacacacagt gactggtaat atagatcact ttttctcatc tccatctata agttctcata
    13021 ttccttcatt atcaatttat tctgcaatag gtatagaaac agaaaatcta gatttttcaa
    13081 aaaaaataat gatgttacct aatgctcctt caagagtgtt ttggtgggaa actggagcag
    13141 ttccaggttt aagatcattg gaaaatgacg gaactagatt acttgattca ataagagatt
    13201 tatacccagg taaattttac tggagattct atgctttttt cgattatgca ataactacat
    13261 taaaaccagt ttatgaagac actaatatta aaattaaact agataaagat actagaaact
    13321 tcataatgcc aactataact actaacgaaa ttagaaacaa attatcttat tcatttgatg
    13381 gagcaggagg aacttactct ttattattat cttcatatcc aatatcaacg aatataaatt
    13441 tatctaaaga tgatttatgg atatttaata ttgataatga agtaagagaa atatctatag
    13501 aaaatggtac tattaaaaaa ggaaagttaa taaaagatgt tttaagtaaa attgatataa
    13561 ataaaaataa acttattata ggcaatcaaa caatagattt ttcaggcgat atagataata
    13621 aagatagata tatattcttg acttgtgagt tagatgataa aattagttta ataatagaaa
    13681 taaatcttgt tgcaaaatct tatagtttgt tattgtctgg ggataaaaat tatttgatat
    13741 ccaatttatc taatactatt gagaaaatca atactttagg cctagatagt aaaaatatag
    13801 cgtacaatta cactgatgaa tctaataata aatattttgg agctatatct aaaacaagtc
    13861 aaaaaagcat aatacattat aaaaaagaca gtaaaaatat attagaattt tataatgaca
    13921 gtacattaga atttaacagt aaagatttta ttgctgaaga tataaatgta tttatgaaag
    13981 atgatattaa tactataaca ggaaaatact atgttgataa taatactgat aaaagtatag
    14041 atttctctat ttctttagtt agtaaaaatc aagtaaaagt aaatggatta tatttaaatg
    14101 aatccgtata ctcatcttac cttgattttg tgaaaaattc agatggacac cataatactt
    14161 ctaattttat gaatttattt ttggacaata taagtttctg gaaattgttt gggtttgaaa
    14221 atataaattt tgtaatcgat aaatacttta cccttgttgg taaaactaat cttggatatg
    14281 tagaatttat ttgtgacaat aataaaaata tagatatata ttttggtgaa tggaaaacat
    14341 cgtcatctaa aagcactata tttagcggaa atggtagaaa tgttgtagta gagcctatat
    14401 ataatcctga tacgggtgaa gatatatcta cttcactaga tttttcctat gaacctctct
    14461 atggaataga tagatatatc aataaagtat tgatagcacc tgatttatat acaagtttaa
    14521 taaatattaa taccaattat tattcaaatg agtactaccc tgagattata gttcttaacc
    14581 caaatacatt ccacaaaaaa gtaaatataa atttagatag ttcttctttt gagtataaat
    14641 ggtctacaga aggaagtgac tttattttag ttagatactt agaagaaagt aataaaaaaa
    14701 tattacaaaa aataagaatc aaaggtatct tatctaatac tcaatcattt aataaaatga
    14761 gtatagattt taaagatatt aaaaaactat cattaggata tataatgagt aattttaaat
    14821 catttaattc tgaaaatgaa ttagatagag atcatttagg atttaaaata atagataata
    14881 aaacttatta ctatgatgaa gatagtaaat tagttaaagg attaatcaat ataaataatt
    14941 cattattcta ttttgatcct atagaattta acttagtaac tggatggcaa actatcaatg
    15001 gtaaaaaata ttattttgat ataaatactg gagcagcttt aactagttat aaaattatta
    15061 atggtaaaca cttttatttt aataatgatg gtgtgatgca gttgggagta tttaaaggac
    15121 ctgatggatt tgaatatttt gcacctgcca atactcaaaa taataacata gaaggtcagg
    15181 ctatagttta tcaaagtaaa ttcttaactt tgaatggcaa aaaatattat tttgataatg
    15241 actcaaaagc agtcactgga tggagaatta ttaacaatga gaaatattac tttaatccta
    15301 ataatgctat tgctgcagtc ggattgcaag taattgacaa taataagtat tatttcaatc
    15361 ctgacactgc tatcatctca aaaggttggc agactgttaa tggtagtaga tactactttg
    15421 atactgatac cgctattgcc tttaatggtt ataaaactat tgatggtaaa cacttttatt
    15481 ttgatagtga ttgtgtagtg aaaataggtg tgtttagtac ctctaatgga tttgaatatt
    15541 ttgcacctgc taatacttat aataataaca tagaaggtca ggctatagtt tatcaaagta
    15601 aattcttaac tttgaatggt aaaaaatatt actttgataa taactcaaaa gcagttaccg
    15661 gatggcaaac tattgatagt aaaaaatatt actttaatac taacactgct gaagcagcta
    15721 ctggatggca aactattgat ggtaaaaaat attactttaa tactaacact gctgaagcag
    15781 ctactggatg gcaaactatt gatggtaaaa aatattactt taatactaac actgctatag
    15841 cttcaactgg ttatacaatt attaatggta aacattttta ttttaatact gatggtatta
    15901 tgcagatagg agtgtttaaa ggacctaatg gatttgaata ttttgcacct gctaatacgg
    15961 atgctaacaa catagaaggt caagctatac tttaccaaaa tgaattctta actttgaatg
    16021 gtaaaaaata ttactttggt agtgactcaa aagcagttac tggatggaga attattaaca
    16081 ataagaaata ttactttaat cctaataatg ctattgctgc aattcatcta tgcactataa
    16141 ataatgacaa gtattacttt agttatgatg gaattcttca aaatggatat attactattg
    16201 aaagaaataa tttctatttt gatgctaata atgaatctaa aatggtaaca ggagtattta
    16261 aaggacctaa tggatttgag tattttgcac ctgctaatac tcacaataat aacatagaag
    16321 gtcaggctat agtttaccag aacaaattct taactttgaa tggcaaaaaa tattattttg
    16381 ataatgactc aaaagcagtt actggatggc aaaccattga tggtaaaaaa tattacttta
    16441 atcttaacac tgctgaagca gctactggat ggcaaactat tgatggtaaa aaatattact
    16501 ttaatcttaa cactgctgaa gcagctactg gatggcaaac tattgatggt aaaaaatatt
    16561 actttaatac taacactttc atagcctcaa ctggttatac aagtattaat ggtaaacatt
    16621 tttattttaa tactgatggt attatgcaga taggagtgtt taaaggacct aatggatttg
    16681 aatactttgc acctgctaat actcataata ataacataga aggtcaagct atactttacc
    16741 aaaataaatt cttaactttg aatggtaaaa aatattactt tggtagtgac tcaaaagcag
    16801 ttaccggatt gcgaactatt gatggtaaaa aatattactt taatactaac actgctgttg
    16861 cagttactgg atggcaaact attaatggta aaaaatacta ctttaatact aacacttcta
    16921 tagcttcaac tggttataca attattagtg gtaaacattt ttattttaat actgatggta
    16981 ttatgcagat aggagtgttt aaaggacctg atggatttga atactttgca cctgctaata
    17041 cagatgctaa caatatagaa ggtcaagcta tacgttatca aaatagattc ctatatttac
    17101 atgacaatat atattatttt ggtaataatt caaaagcggc tactggttgg gtaactattg
    17161 atggtaatag atattacttc gagcctaata cagctatggg tgcgaatggt tataaaacta
    17221 ttgataataa aaatttttac tttagaaatg gtttacctca gataggggtg tttaaagggt
    17281 ctaatggatt tgaatacttt gcacctgcta atacggatgc taacaatata gaaggtcaag
    17341 ctatacgtta tcaaaataga ttcctacatt tacttggaaa aatatattac tttggtaata
    17401 attcaaaagc agttactgga tggcaaacta ttaatggtaa agtatattac tttatgcctg
    17461 atactgctat ggctgcagct ggtggacttt ttgagattga tggtgttata tatttctttg
    17521 gtgttgatgg agtaaaagcc cctgggatat atggctaaaa tatatgtttg ataaaaaatt
    17581 attcctgtgc tactaagaaa ttatttttat ataataaata ttgagattta attaaaagtc
    17641 atgtgttatt gtaatacatg acttttagtt aaaatttttc tatcatttaa taatctatta
    17701 ttcttgacta ttttataata aaattcatat atggaaatat taatactaaa taattaatag
    17761 ttgataaaaa atagataata tgctaaaagc aaaaactaat ttagagcctt gtaactgttt
    17821 atttgcaatt ataaaaacat ctttaaacat attgactata atataaaata ttaactataa
    17881 tacaaaacaa tattaattaa ttttctctac agctatccct ggtatagtta tatttccacc
    17941 catagttgat tcataagtta tagttccagc acttatacct cttatagtta tgtaatcatc
    18001 ctctaatact cttgaaggag ttattgattt tttataacta catagtacaa ccttatcata
    18061 atttccagac acagctaatc ttatttgcac ctcatcacca tcttcaataa cttgaataac
    18121 cttaccttca aattttacct ttttgtactt ataatcatca ggtgttctag ctaattggtc
    18181 ataagtaata ccagtatcat atcctttctt ctcctcttct tcttttttgc gttgttcttc
    18241 agccttttta gcttcttcag cttttttagc ttcttcagct ttacgttgat tttcagcttc
    18301 aatagctttc ttttcgtcgt ctttcatttt gaaccatggt tcagcatcag acaatttgct
    18361 atttaaagtt tctatttctg ttttttgaga atctataact gatgtttggt tagaaatgac
    18421 ctcctcatgg tcttcagaac aagctggtga ggatatattg ccaaaaaaca cgcctaaaat
    18481 aaatgccagt agagcaatac cctttgtgct cttaaagaat ttaattattt tcttagagct
    18541 tccttttcct tcattactaa attcgttacc atcatttttt ttagaaaaca taatacaatc
    18601 tcccttttat tagatatatt ttattttaaa agttaaaaac ttaaaataac tagttgacta
    18661 aaaattatat attcatattt tcctaaaata tatttcccct aagcataatt ttacaaaata
    18721 aagtaagaag attcaatata ttttgaaaaa ataagataaa aatgtcagat tataatataa
    18781 ttttcttgaa ggtgaatttt atatattaaa ttaaacataa agtattttat ttgtataaaa
    18841 ctatattatg atgtaattaa gtgactcagt gatttagagg tggtaaaata tatatatatg
    18901 aaagagcata aataaattga tataataaaa aattaaatat taaatattaa ctataataaa
    18961 aaaatatctg gtaataggta taatataaaa agaatatacc agaaagaagg tgaagtatgg
    19021 gagaactaca aaaaattcca ggggtaggga aagcaacaga aaaatcttta atcatgttag
    19081 gatatacaac tattaaatca cttaaagatg caaacccagc acaaatgtat gaaaaggaat
    19141 gtttaatacg tggacagcac atagacagat gtcagttata tgtatacaga tgtgcagtat
    19201 attttgcatc aactgaaaat ccagaaccag aaaaattaaa gtggtggtat tggaaagatt
    19261 aaaaaatcaa aaaagttaga cataaaaaag agttgaatat ccgttttaaa taataaaaac
    19321 taatactcaa ctctttttta gtcgtaaatt ttatgtaaat ctatttgtat ggctaaatat
    19381 acccatcaat aaatgtataa atataccaat caacaaatga ctaaacatac tacctaacaa
    19441 aggattaaat aaattactta acaaagcatt ctaaaacaat taattagaaa ctgtttcttc
    19501 aagccatttt ctacattgct caatatttac catagggtca aaaccttggt cttttccctt
    19561 atttaaaagc atatcacaag ggaaagtatc acattcacca caatgattaa gttccttact
    19621 ttcacagcaa gttttaacct tacattctcc accccaaaat ggtttttcca tagttgggca
    19681 accagtgcaa tttacttgtt ctttttttgt acaactgtca caacagacac cacatcttga
    19741 ttcaaacatg ttcaaacatc ctttccatta tttaaaaata ttataaaatg attttagatg
    19801 aatacaaaag tgcaaatagt tgattttagg tcatctaaat gttaaacata aaattattag
    19861 tactggtaaa tttataaaaa ctattccaaa aatctttttg aatatttcaa aacacaataa
    19921 attacttcaa atccctttga atatatttta cagcattata gattgcttta aatctctttg
    19981 aatattctac aagactatag attagtttaa atccctttga atatatttta caacattata
    20041 gattgtttta aatctctttg aatattctac aagactatag attagtttaa atccctttga
    20101 atatttcaaa acaccataga ttactttaaa tccctttgaa tattctataa cactataaat
    20161 tacttttaac ttttatataa ccataaagtt tttatctggc actttaaatg gagttttata
    20221 tcaaatttgc cttccatcga ataaccaaac ttaatttaat tacacattcc aagagtcaaa
    20281 tattcattca atatatttga taattctatg ttatagctat aattaaatat agattattaa
    20341 agttaaatat ttatttaata tattgaatga ttttttacct atatttaatt aagtttcatt
    20401 atataatata aaaattgttt ttgcattaag ttttaatata atacataata attttaaata
    20461 ataacattat tatatatttt ttaattttag gagtatatct attatatagc gagtagataa
    20521 aacatataaa attcaaataa aagatatata tttttaaata aaatggatat agatttcaac
    20581 taaaaagaat ataaatttca aaccccaaaa tatctagcat ttaccattaa gaaataacat
    20641 cattctttct aaaatttcta ttggcaattt cagaaaatac tatagtagta ataaatgcaa
    20701 cacatattgt aactatagtg tcattaacat ttaaagcaga ttgctttata acccccatag
    20761 cactacctaa catagaataa gggaaaaata taccaacagc aggttttgct actattaatc
    20821 ccataccaaa tagagaaagg caaagactaa taccaatagg agttgcaaaa cttctaatct
    20881 ttatagacat gtataattgt atagaagcta ttgaaataga aataatccaa cctttagcaa
    20941 tccaaccagt caattcttta ggtacactac cagaaaaacc aaacatattt cctgcaacta
    21001 tataaaagat aacaaagaaa acttgtgtaa tagctgtaag tattgaaact actgtaagtt
    21061 tagataaaaa gatatttttt aaattaactg gcagtgtcat tacattattc caattatgat
    21121 tcatatgttc aagcctacaa atatatgcac aaaagattgc gattaagata ggaaagaaaa
    21181 attccccata aaaaagacta acttgagtcc ataagctgta ccattctttt ttgagtatgc
    21241 cttgatttag ataaaaatta ccagaaccaa tcaacacact tataagtggc aaagctaata
    21301 atacatacca tataaaagaa ttttttaact ttagaatttc tcccaaaact gacttcctta
    21361 acatttaaat ccctcctata cttctttttt taataaatga tttcttccaa tataataaat
    21421 tagcattcca attaagaaaa ctaaaatcgt aatagaaaaa ttcatatcaa ttctatagac
    21481 agatgaacct aaaacattat cataagcata acttaaaggt gataactcta ggtaataaga
    21541 ccatataaac aatctacgaa taccttgata aaacaaacct gatgtcaaac ctataaaact
    21601 accaatcatg ccaaatgtaa ttgcaatcat ttgatttgga attacagttg caatccatag
    21661 gtgtagtgca atgatagcaa tacttacaag aattgttcct aatgtgtatt ttaaaagtaa
    21721 agatacagaa attgactcaa taacccctct aaaatgtcca aacaacacaa tagataacac
    21781 ttgcatcaat acaggtatag ataccaaaat agcagaacat aaaaatttag ataaataaat
    21841 actatttaaa gatgtcacag aagattttaa aagcttccat gtatctcctt tatgttctat
    21901 atcgcttata cgtgatgtaa ttattgatat taagatagga aagaatagac catttaaact
    21961 tgcaaaactc ataaacatat aggaatattc aaatccacct aacatatctg gattacgtgt
    22021 aatagctcta tttgaattta tgatacacca cattatttca acagctacaa ataaaaaggt
    22081 catcagaata atcttctttc ttttaagttt ataaaattct agaccaaggt atttcattat
    22141 aaagtcaccg ccttaccagt aagttctaag aatatatctt ccaagctttt tttatgttct
    22201 tctattctaa atacatctat atttgaatgg acaagcatac catttatttt agccacatct
    22261 gagtctttta aattttcaat agtcaagtac ccatcttgta taagtgacat atatccttta
    22321 gtatttaata gtttttgtgc agatacaaca ttcttagttt tgatagcaat ctgactttga
    22381 cctctattat gaagttcttt tatgctatct tgaaaaacca gttcgccatg atttattatt
    22441 cctattgatg tggcaatttg gtcaatttca cttaaaagat ggctagagat tagaacagtc
    22501 atcccgtatt tttctggtag agattttatt aattctctaa tttcctcaat ccctgctggg
    22561 tcgagtccat ttgtaggttc atcaagtatt aataatttgg gaaacttcat tattgccata
    22621 gcaataccaa gacgttgctt catacctaaa gaatattgac taactttttt atttttttga
    22681 ttttctaacc ttacaatttt taatgcttca tttacatttt ttttaggtaa atctaagagt
    22741 ttttgcatta tggtcatgtt ttcaagccct gtaagatgac catagtatga tggagattct
    22801 atcaaagaac ctatatcttt taaaattgat attctatttt tttcatttaa ctctttacct
    22861 aaaatattaa tatttccttg tgtgggttta gcaagtccaa gaagcatttt aagagtggta
    22921 gattttccag ctccattagg acccaaaaaa ccatatacag ctccttgtgg aactcttagg
    22981 tctatatcct ttactctaaa tattttattg tattcctttg aaagtctttg tgttctaatt
    23041 atcatttcca tatgtttcat cccctttttt aatctgtgct tattgattct tttaacttat
    23101 atcagtataa taacttggtt acatttcaat tagctgaaat ttaccttaca ataaccttaa
    23161 aagtctattt aggcaaagtt gtgaaaatgg gtatatggta tactaaagga gggtgatgat
    23221 tatggagtca ataaaagata aaaaaatact tgtggttgat gaccacaagg aattattaaa
    23281 aatgatagat gaaatcctga gaaaagaggg gtttagtaga gtatttttag catcaagtta
    23341 tgaggaagct gttagggtat ttagaaatgt aaagccagat tgtgcaattt tagatgttgt
    23401 attgccagat ggagatggtt tttctattat gagaaaaatt agagagacat ctaaaatacc
    23461 agtgattttt ctctctgcaa gaggtgaaga tgaagataga ttgattggtt tgggcttagg
    23521 ggctgacgat tatatagtaa agccattttt accaaaggaa cttactttaa ggcttgtggg
    23581 tatattaaat cgtgtatatg tgcctataga agaagaggag ttgccagtat ttaaactggg
    23641 agattctgtt gttgtgaatt taaatagtgc atgtgttgag aaagatgggc aagagatttc
    23701 tttaacagct aaagagcatt cgttgctttt aaagttgtat gaaaataaag ggagaattgt
    23761 tactaatgat tcactatgtc aagccatttg gggagatgat atgtatggat atgaaaatac
    23821 ccttatggtt catgttagaa gggttagaga gaagatagaa aaaactccat ctacacctaa
    23881 acatcttatt acagtaagag gtcttggata taagttgatg attaattaat tttagatatt
    23941 agaataaaag aaatcaagag gagtgattta attttggata gtgtaaagta catattgagg
    24001 cgatttatag gttcaagtat cttgatttca ttacttttta ttgtattaaa tatagcaggt
    24061 tttcttctgt tttcatatat gctatttgta tctaaatact ctgataattt agatacaagt
    24121 gatagcagag aaataaaatc aaacttgatt atgattgaag ataatttgat tttaagaaat
    24181 ggaaagttta ttttagatga ttctagtaag gaagtactac ataaaaataa agtttgggca
    24241 atgttaattg ataacaatgg agatagaata tggggatttg atttaccaaa agaaattcca
    24301 agtcattata ctttgacaga tgtagcaaag ttttcaaggt tctttttaaa ggactatcct
    24361 gtgtatgtat gggaacatcc aaatggaata ttagtaacag gttatccaaa agacaagtac
    24421 actaagttta accttactta tacggttaag gaaatagaac aatttcctat gttaataata
    24481 ggtatgtttt tggttaatgc aatagttact tttttaatag ctctattcat aggtttaaaa
    24541 atggtgaaat ctataaaacc aattattact ggcataaagc ttatgtcaaa aggagaacca
    24601 gtacttttac aagaaaaagg aatactaagt gatatttcaa aatctattaa tactgtatct
    24661 aaagagttgc aaatgaaaga tgaaaaactt aggaagaaag aggaagctag gtctaactgg
    24721 attgcaggaa tatctcatga tataagaact ccactttcaa tgataattgg atatgctgga
    24781 gaacttgaag aaagcagtag cctttcaaat agagaacaag agcaagtgtc tattatatgc
    24841 aatcaaggga ttaaaatcag agaattagta aatgacctaa atttagtgtc taaacttgaa
    24901 tataatatgc aagcattgaa ttgtgataag attagagttt ctgcgttttt gagagagctt
    24961 atttctgagt ttataaataa taatttggat aatgactttt acgttgaatt tgatatacta
    25021 aatgaagata ttattataga tgcagataaa aaattattaa aaagggctat tagtaattta
    25081 atacaaaata gtataactca taaccaacaa ggatgtaata taagggttac ctctaagtct
    25141 gatttagatt tttgctatat aattgttgaa gatgatggaa aaggtatgag tcaagaaaga
    25201 attaatagtt tattaaagag ccaaaatgga ctaagtgagg catatgccaa aggtcaaaat
    25261 catggtctag gtattttgat agtatctggc attattaatg cacatagagg taaattagat
    25321 attacgagca ttgatagtca gggactaaaa acaatactaa aattacctct aattaagtga
    25381 tattttataa aataataaca gataagaatg aagaatgaaa atattttgaa aataatttta
    25441 ttctttattg acaaattaac tctattagag tagaataagg ataaattctt ttcttgtcaa
    25501 gttttattaa agtaaagagt aacttgtgct ttatgcgctt atggctgagt tactcatggt
    25561 atcaaaattt tcatagaaat aatattttgg aattattgta aggttattaa attctttaga
    25621 taattccatt ttttttattt tttagttaat atttaatttt attattgaca aaaaaattct
    25681 actaaaatag aattatggta tagtgttttt taattttaag aaaaaatata atgacatcct
    25741 aaaaaagttt taattatacg ctgttctgtg tagttataat agcttctatg taactattgt
    25801 aagtatatta ttatagttat ttatttaaag tacgataaat gttttaaatt gaagtatttt
    25861 gcgccattga gttctactaa agtagagttt ttgtgtagta tttttagaaa taacatatat
    25921 atatatgtat ttatgttgaa atatatggga aatttataat ataattaatg aaaaaattta
    25981 aactttgtaa tatataaata aaaaaactct aaaatagaag gatataatta tatagaatgt
    26041 ttcaattaaa caaggcaaaa actataatct taaaattaac attattctta agaagtaaga
    26101 aatttaagaa gtaaaaagtt tagttataag tggtagtaag caagaaattt aagaagtaaa
    26161 aagtttagtt ataagtggta gtaagcaaga aatttaggaa gtgggaagtt tagttataag
    26221 tggtagtaag taagaaattt aggaagtggg aagtttagtt ataagtagta gtaagtaaga
    26281 aatttaggaa gtgggaagtt tagttataag tagtagtaag taagaaattt aggaagtagg
    26341 aagtttagta ataatttgta gcaagtaaga aacttaggaa gtgggaagtt tagtagtaat
    26401 aatttatagc aagtaagaaa tttaggaagt aggaagttta gtaataattt gtagcaagta
    26461 agaaatttag aaagtaggaa gtttagtaat aatttatagc aagtaagaaa tttagggagt
    26521 aggaagttta gtaataattt atagcaagta agaaatttag ggagtaggaa gtttagtaat
    26581 aatttgtatc aagtaagaaa tttagggagt aggaagttta gtaataattt atagcaagta
    26641 agaaatttag aaagtaggaa gtttagtaat aatttatagc aagtaagaaa tttagaaagt
    26701 aggaagttta gtagtaattt atagtaagta agaaatttag taagtgagaa gtttagttat
    26761 aatttatagt aagtaagaaa tttagtaagt gagaagttca gtaataagtt gtagtaaata
    26821 aaaagtttag gaagtaggaa gttcagtaat aagttgtagt aaataaaaaa tttaggaagt
    26881 aggatttact gaaatacaaa tatttaaaat caaagagttt aaaagaaagg agagctactg
    26941 gacaagtatc caaaatagat agctgagtac aaaatatttc ataaaattaa gcaatataat
    27001 acaagagaat tttgacatta tttatatata tttaccgtgt acaaatagct aaatttagga
    27061 ttaagattga aaaatatctt ataagtctga gcttatataa atccgtataa gatgtattta
    27121 atcgaaaaat tttattatgg atattgcttg tctatattct gagtagcaag gttttatcat
    27181 gactataaaa aagttgccac aatcagaatt aaagataatg aaatttatat ggaaatcaga
    27241 ttccaaagta acatctagag atattgtttt aggaatggag caaaaatatc aatggaaaca
    27301 aacgacaact ttgactcttt tatctagatt agtagtaaaa agatttctaa attctcaaaa
    27361 gatagataaa tatacgcatt atgaagtttt aattaaagaa aaggaataca taggagttga
    27421 gacaagagat ttttttagaa atattcatga ttcttctata aaaagtttat tgttgtcatt
    27481 acatgaaaat attaatttga gcaaagatga tatcttactt attgaagagt ggataaaaaa
    27541 tctaaaagaa gaggagaaag acgtataagc aaacgtattt cttatagcat caatataaag
    27601 tgtatgtaag tgtatttatg taatatatta gtctacttaa gtaatatgac aaatgattat
    27661 aagtggatta tgatactata atgtgaatct aaataaatta aataaaaatt aatagtggat
    27721 attctattta attaaattta aaaagctacc tttacttata agtataatta aggtagcttt
    27781 ttaaatagag gtagtgtaat taaagagaat ttttaaatat aaattgtgaa gctttaatat
    27841 agatgataat atatattttg caaatatgac acagtgtttt cgtatttaag atgataaaat
    27901 aaataggaat taatgatgat attgaaaaaa taaaaataaa ggattaatat aagatattaa
    27961 agagatagag ataaaagtgt taacagaaga tattgaaaaa ataaaagatt gatataagat
    28021 actgaagagg tagaaacaaa agtattaata gaagatattg aaaaaataaa aacaaaagat
    28081 tgatagaaag tactgaatag atagagataa aagtgttaat agaagatatt gaaaaaataa
    28141 aagattgata taagatactg aagaggtaga aacaaaagtg ttaatagaag atactaaaaa
    28201 agtaaaaata aaggattaat aaaaggtact gaagagatag agaaaaaaat gttaatagaa
    28261 gatactgaaa aaatagaaat aaaaaattag gaggaaaatt tatgaaatat caggtttcat
    28321 tattagcagt gaaggatatt gaagtatcaa aaaaatttta caaagaatta tttgagcaag
    28381 aaattgaatt ggacttaggt aaaaatgtaa catttaaagg aggatttgct atacaagaag
    28441 acttcgcatg gcttacaggg attaatccag atacaattat acaaaaatca aataatatgg
    28501 aactttattt tgaggttgat gattttgacg aatttattga aaaactcaat tattataaag
    28561 atgtagagtt tgtccataag ccactaaaac atgaatggca acaaagagta gtgcgtattt
    28621 atgacccgga taagcatatt attgagatag gagagtctat ggaagttata gctagaagat
    28681 atttatctga gggatactct gtagaagaaa cttctaaaat aattcaacat ccaattaaat
    28741 ttgtaaagca gtgtgaaaat agtaaggaat gattgtttaa attataatta tttagtagaa
    28801 attaattgta atagaactaa ttcaagataa tatataggac tagtatttga c
//
LOCUS       DAMJWS010000049        28810 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMJWS010000049 DAMJWS010000000
VERSION     DAMJWS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.049"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            435..1127
                     /locus_tag="PC399_002228"
     CDS             435..1127
                     /locus_tag="PC399_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593085.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1323..3383
                     /locus_tag="PC399_002229"
     CDS             1323..3383
                     /locus_tag="PC399_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2593086.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3589..3912)
                     /locus_tag="PC399_002230"
     CDS             complement(3589..3912)
                     /locus_tag="PC399_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF2593087.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3905..4606)
                     /locus_tag="PC399_002231"
     CDS             complement(3905..4606)
                     /locus_tag="PC399_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF2593088.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4884..6269)
                     /locus_tag="PC399_002232"
     CDS             complement(4884..6269)
                     /locus_tag="PC399_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2593089.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALVEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6377..6520)
                     /locus_tag="PC399_002233"
     CDS             complement(6377..6520)
                     /locus_tag="PC399_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593090.1"
                     /translation="MYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRF
                     EIN"
     gene            complement(6715..7137)
                     /locus_tag="PC399_002234"
     CDS             complement(6715..7137)
                     /locus_tag="PC399_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF2593091.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            7615..10518
                     /locus_tag="PC399_002235"
     CDS             7615..10518
                     /locus_tag="PC399_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2593092.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISVSEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            10551..10997
                     /locus_tag="PC399_002236"
     CDS             10551..10997
                     /locus_tag="PC399_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2593093.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            11120..11422
                     /locus_tag="PC399_002237"
     CDS             11120..11422
                     /locus_tag="PC399_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2593094.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            11603..13009
                     /locus_tag="PC399_002238"
     CDS             11603..13009
                     /locus_tag="PC399_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2593095.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            13353..14411
                     /locus_tag="PC399_002239"
     CDS             13353..14411
                     /locus_tag="PC399_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2593096.1"
                     /translation="MGDKMRASVLYSVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            14510..15196
                     /gene="araD"
                     /locus_tag="PC399_002240"
     CDS             14510..15196
                     /gene="araD"
                     /locus_tag="PC399_002240"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF2593097.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            15214..15861
                     /locus_tag="PC399_002241"
     CDS             15214..15861
                     /locus_tag="PC399_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2593098.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(16034..17401)
                     /locus_tag="PC399_002242"
     CDS             complement(16034..17401)
                     /locus_tag="PC399_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF2593099.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(17591..17679)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            18107..20227
                     /locus_tag="PC399_002243"
     CDS             18107..20227
                     /locus_tag="PC399_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF2593100.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKTIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKTCNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNILEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            20331..21584
                     /locus_tag="PC399_002244"
     CDS             20331..21584
                     /locus_tag="PC399_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2593101.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSKMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(21737..22798)
                     /gene="ytvI"
                     /locus_tag="PC399_002245"
     CDS             complement(21737..22798)
                     /gene="ytvI"
                     /locus_tag="PC399_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2593102.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            23072..23812
                     /locus_tag="PC399_002246"
     CDS             23072..23812
                     /locus_tag="PC399_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF2593103.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            23824..25602
                     /locus_tag="PC399_002247"
     CDS             23824..25602
                     /locus_tag="PC399_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2593104.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            25769..26686
                     /gene="pfkB"
                     /locus_tag="PC399_002248"
     CDS             25769..26686
                     /gene="pfkB"
                     /locus_tag="PC399_002248"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2593105.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            26723..28642
                     /locus_tag="PC399_002249"
     CDS             26723..28642
                     /locus_tag="PC399_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2593106.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT        11455 a         3151 c         4787 g         9417 t
ORIGIN      
        1 aatttaaata agtagctatt attgcagtga aatgaatgtt tctaagtata tttgggtgga
       61 agaatggtgg aagaaaatta gtgaaatgta attcttccac cattcttcca cccaaaaaat
      121 aaaaactgtt caataagaat taatatacat caattcagct gaatgcaatc aaaatactta
      181 aatgaaagta atctgaaaac aacataattc aaatcaattt attcaaataa acctcaattt
      241 atcttaaatc ttaagaaata tttaagaatt aggataatat tttcttaaga tttatatttt
      301 attatatagt tataagggaa agttacaaaa atgtaagcca aagtaatata acaatttagt
      361 cattgttgag aaagggagtg aaagatattg tataatatag ataagtatta ttatacaata
      421 gaggaagtga aaatatgtac aatatattag tagtagacga tgataaagaa attgttgatt
      481 cgatagaaat atatcttaaa tcagaaggat ttaaaatata taaagcatat gatggaatga
      541 aagctttgga aataataaca gagaatgaca ttcatctaat actaatggat ataatgatgc
      601 ctaaattaga tggaatcaaa gcaactgtaa aaatacgaga agaaagaaat attccaataa
      661 ttttaatttc agcaaaaagt gaagatacag ataaaataat aggattaaat attggagctg
      721 atgattatat aacaaagcca tttaatctat tagagcttat agctagggtt aaatcaaact
      781 taagacgata tgtaacttta ggtaattatg aagatacatg taaagaaatt ctaaaaagtg
      841 gagctttgga gttaaataca ctaacaaaag aagttaaaat tgatggtgaa aacataaaaa
      901 tgactccaat tgagtataaa ataattgaac ttcttttaga aaataaagga cgagttttct
      961 caatagatga gatatacgaa aaagtatgga atgaggaaag ttttaattca gaaaatacag
     1021 tagcagtaca tatcagacga ataagagaaa agattgaaat aaatccaaaa gaaccaagat
     1081 ttttaaaggt ggtgtgggga gttggttata agattgaaaa attttagtgg aaaaattata
     1141 aaagaaaaga gtttaatatt taattgcatt ttgattttag tcatttttac atttacacaa
     1201 atgttaggaa ttttaatatg ttttaatttt ttctctgaga gtttaattta ctctatgggg
     1261 attatgcttt tcttaaactt tattttatat aaaatactga caatgtagat gaggtgggca
     1321 acttggagaa atatagagta ttaaagttag tttcattaat cataatatct atctcatttc
     1381 cattaactat attgttggtg acaataggac ttgctgagcc taaatacaat atacaaaaaa
     1441 cttctacaaa gagtgggatt tttgatatat ctgatgattt ttatgatagt aatatatttg
     1501 acgaaggaac aatggcaagt ctattaccca agtttaagct atctgtaggt ggtaaattaa
     1561 ctagtaaaga aaaaggtaaa ttccaacaag aaggtaaaag tatagattta agtagtagtg
     1621 attctgagta tgataaaatc atgattaatg aacaagcttc tgaagaatat ttaagttatc
     1681 taaaaaatat aaaagtgtat gttattaata catacacagg agtattttat acaaatacgt
     1741 catatgctag tttatatgat tttaaacaaa aaactaagag ctattgtaat gtagagataa
     1801 taaataagat aggaaagagc tcatatatta aaacaataaa tggagaaaaa tttgaactga
     1861 ataatctgag tctggaacta aatgaagtag atgaaggttt tgaggcatat gtatcttttc
     1921 cagaagagcc aactatagaa gatggaatag tatatactaa ttttcaaata tttaaacaag
     1981 ctacagaaaa agtaagatta ttttttgtag ctagtttaag tttagcaatt atatgtttat
     2041 tatctataat tttatatatc aaaataaaag gtgaagtttt aaaaagaaat aatgtatttt
     2101 tactaatata cagtaagata cctatggaat ttaatgtaat attagtatct atttcaattg
     2161 caattttaac tatatatgct cttgctgtat cttatcatat tgatgtaatt gcattagttt
     2221 ctttatcttt actgatagta tcaaataata tattttttat aaatgtgcaa ttgagctatt
     2281 tagaaaataa aatgtgtata tttaagacaa gtattatagt aagagtgatt ttatggttaa
     2341 taaaaactgt aagggaggtc agtaacgcta taaagaaagt atctttagca aggaaagtta
     2401 ttgttatagt gataatatct ttagctttaa tcatggcaat tagcactcga agtataggta
     2461 tgggattact gttttctata tgtatttttt taggatttac aatatatata actaaaagac
     2521 tttcatatct tagttacgta atagaaggta cagaaagaat aaaaaaggga gaattagatt
     2581 ataagataaa aattactggg aatgataatt ttacgtcttt agctgaaaat ataaataata
     2641 ttggagaagg tcttgataga gctatagaag agcaagtaaa aagtgaaaga atgaagtctg
     2701 accttataac aaatgtatct catgatttga aaactccact tacttctata ataaattatg
     2761 tagatttaat taaaaaagaa gaatcaatac aaccagagta tataaatgat tatataaatg
     2821 tacttgagtc aaagtcaaaa agactaaaat tattgataga agatttattt gaagcgagta
     2881 gagtaagtag tggcaatata gaattacaaa ttagcaagat tgatttagtg caactattaa
     2941 gacaaagtat aggtgaactt gaagaaaaat tatctaaaaa taatttatat ttaaaattaa
     3001 atgttccaaa tgataaggtg tatatatggg cagatggaag aagaatgtat agagtatttg
     3061 aaaatctttt atctaatata gcaaaatatt ccttagaaga aactagagtc tatattgatg
     3121 tatatgacta tggtgaaaat gtaaaggtaa ctatgaaaaa tatatcttct tatgaattaa
     3181 attttgaccc aagtgagatt atggaaagat ttaaaagagc tgatgaatca aggaatactg
     3241 aaggaagtgg attagggctt gctattgcaa gagatttagt agctttacaa ggtggaaaat
     3301 tttatataga aatagatgga gatttattta aagcaaattt agagtttcaa aaatatgaag
     3361 aagaaataac aaatatggaa tgagatagta tttaagtata tatatttttt ttactgtcga
     3421 aagcttttat tataatatat tagaatattt acagtagaga atgaagagta aaaacagagt
     3481 aactttaagt cactctgttt tttatttaaa atcaataata taacaactta aattaaaatt
     3541 gtacacatag ttttaaaata acttatacaa taaaacatta tccaaatatc acaagataat
     3601 tccaaataaa tatatagata tcactcctgt aataatagaa ataccaatag atttaaactt
     3661 atatgcaaca agaagtacaa tcataaaaga tataatttta gggttagcta tagataaata
     3721 tagactatct ccattaaaaa atacatcttt agcagttaag gaagtaagta ttgctacagg
     3781 tataaatttc atgatttttt tcaaaaaatc aggcatctct ttttttgaaa aaattaacat
     3841 aggaagcact cttggtagat atgtaacaat gtacattcca attataataa aaaatatata
     3901 attattattc ataagattcc tcctttttac agtaagattc ttctataaag aaaccaatta
     3961 aagatgctaa aattgatgca actattatta ctaaattgct tttgaataat gcatataaaa
     4021 taacagaaat aatgattgat gatataaata ctacaatata aatcttattt tttatttgag
     4081 aaactattaa agctataaac atagaagtca atatgaagcc agaaatagaa ctgtctatag
     4141 atatactttc tcctaaaaat gctccaataa aattagctaa aactgatgaa attaagcaga
     4201 agaaattaag ataaatagca tttctatcag accaatttcc agcagtaaat ttttcataat
     4261 taactgcaaa agtttcatct gttatagtat gacaaaatgg caatataaat tttggacttt
     4321 tattttttat gtacatgttc aaattagaac tcattagaaa atgtcttaag ttaactataa
     4381 atgtagtcaa aacaatagat gttacacttg caccagtaga aatcattgaa gctgcaatga
     4441 attgagaact tcctccaaat actaaagtta ccattagtgc tatttgaaaa ggtgaaagac
     4501 cagatttttg taatagtatt cctcctacta tacctatagg tatataacct aagaatatag
     4561 gaattgaagc aataaagctt tcttttaatt gttgagttct ttccacaata aatcactcct
     4621 tttgtattaa atgtattaaa gtattagtaa aacagaaaaa tagattaatt gtataattgc
     4681 aattataatt aaaaataaat ttggattaat atataaaata ctatacaaca aatagataat
     4741 cttaaacggt tattaaattt tatctatttg ttattatgat gtaataatac atattaatct
     4801 ctataagtta attactatct ggtgtaattt tattttaaca acaaaaaaaa atacacacaa
     4861 ttaaaatatt gtgtgcaata caattatttt ttctcttcta atagtctttt taatacgctc
     4921 aaaccagctt taagttcttc tgtactttct gtagcacata cagctattct tattgcagaa
     4981 attggttttt cttttccaac agcaaaacgt tcagaagcat agacttgtac accttcttta
     5041 agtgcttgta tctcaaattt ttctgccatt attccatcag gtagaatcaa ccatctaaat
     5101 atacattctt catttcccaa aagattatat ccagataaat attgattaat aatcttgttt
     5161 cttcttcttg cagatttacg gcgtgcatta attaatttat cggcttcacc tgatgtaatc
     5221 atgcgataag caatttctgt taaaaaataa gattgagata gattaatgtt gtataatgcg
     5281 cttgataatg cctctcgata ctgtttaggt gtagagatat atgctagccg taaactaggt
     5341 gcaataattt ttgataaact cgtaatataa attgtttgat ttggcagata ggaagccact
     5401 ggatttaaat gtgtttcatt aagtaaacta tgaatagcat cctcaataac aattaaattg
     5461 tatttagatg caatatttgc aatcattttt cgtccatttt gtgacattat atgtgtagtt
     5521 ggattttgat aatctggtat tatgtataga cctttaatat tttcattttt acaagcatat
     5581 aatagtcctt cttcactaat ctcattatct tcttgtttaa ttggaattag ttgaatccca
     5641 agcattttag ctgctgtctt tattccagga taagttaatg ggtctacacc tattctatct
     5701 ccatgttgaa acaaaccagc taaaatagca acaattgcat tttgtccacc acttgcagga
     5761 agtaagctat ctgatgtagt ttcaagccca gctttataaa ttaatttggc aatagcttct
     5821 ttttgccatt tagcaccatc tctttgtcca tattggaata tagttttaaa atctatttcc
     5881 ttaattatgt gtttaaacaa agtattcatt tcttctagcg tgaaatctgg attcattgtg
     5941 cccatttcaa taaatgtaat tttattatta ctggacatca agaatagatt tgaacgtgta
     6001 tcataagata caaaagttcc acttcctgta actccactta ttagaccttt tttttcacaa
     6061 attttaaaag ctctagagat agtactcaca ttaacatcta agaaatctgc caattcacgt
     6121 tggggtggta atttagtacc tggtaataaa aatccattgt tgatatcttg ttctagttgt
     6181 tctacaagcg ccttatataa tatttgccct tttgaaggtc tctttggttt ccagttcata
     6241 ggataatttt caaaagaatt aattggcata agttttcacc tcacattatt aatttgattt
     6301 tttctttatc taaaacaata aattctaaag atacaaataa aaactgatag gaaataattc
     6361 tatcagtttt tgtcttttaa ttgatttcaa atcttcctat aatttttata ccttctttat
     6421 cataaagagg gattgttttt ttaaatatat taaaatttct ttttcttgta tataaaacag
     6481 cttcttctgg tgtctttggt tggttatcac aatcgtacaa atttcttttt tttaactaaa
     6541 agcaataata cataaaatta ttattgctaa tgatactctt aatgtggtat atagtcatag
     6601 tgtcacatgt ctatcaatag ttttttcagt aattattgta acgaatagta ggactcataa
     6661 aatacttaga aatatgtttt tagataaatt ttaaatatat aatctaactt atatctataa
     6721 attaacacta ttattctcca attccatttc tagttttttt ataaatttat taaattcttc
     6781 tggttgcctt tttccaaaca atactcttac aagagctgaa ttgatttttc caaatatatt
     6841 cctcttgctc tcactttcaa taaaaataag ttttgtattt tctaagtcaa tatgtactaa
     6901 ttcatatcta gtaatagtac tttctctttt attgcttgtt tttatctcat atactctatt
     6961 tttttcgaaa ttagttattt caaccttaaa tcttttatta tttttttgct gtacaaatgt
     7021 tcctactgct tttttctcat taaaattagg aaaatttagt cttgcagatt ttgtaaatat
     7081 attaaatatg tcttttgcag aatattttaa taaaacttca gttttaaatg tattcatatc
     7141 gttttccctt ctgtatataa atttaatatt tataagttaa tttttaagtt aaaatagtat
     7201 aatatgccat attaaatcat gttaaaacta tttatatagc taaaatctta cctatataag
     7261 tatgtatact atcatataaa aataaaatct taaaatagaa agggttaaaa acacagttta
     7321 ttataatttt tattgttgag tagatacgta ctattatata tgattgagca agtttttcaa
     7381 tcataaattt ataaagtatt ttgcattaaa atttaaacaa agaattaaat tatttaagat
     7441 tatgaataac taatacattt agtaaattac taaatataca aaatgtaaat acttatcaaa
     7501 atgtataaat acttatgaca tctagttact tacagggttt aagtagaaaa aaatatcaaa
     7561 gtaagtatgg catgatagtt gcttatatat ttatcaagaa aggagttatt aagcatggag
     7621 ttgagagata aacttgtaaa catcataaat acagaaaata aaaaagatcc attaacagat
     7681 gttcaaatag caaagttttt aagtacaact agagagaaca taactaattt gagaaaagaa
     7741 cttaatataa gtaattcaag gcaaagaaga tatccttatt taaaatcagc aatttcggca
     7801 atattacaaa agaataagaa tatttctgta tcagaaatta caagggaatt gatgacagaa
     7861 ggctttaata tatctagaag agttgtggag gaacttttac ctaaagaaag tttagaaatg
     7921 gatgtagtag aggaatctga agaagaatcg aatgacccat ttgaaactct gataggtaat
     7981 aaagggagtc ttagaaatgc tgtagagcag gctaaatctg ctatattgta tccaccaaaa
     8041 gggttaccaa cgttgattat tggggaaagt ggagttggta agagtttatt ttctagacat
     8101 atgtatgagt ttgcaaagca aaaaaagata gtaaaagaaa gctccaattt tgttgtattt
     8161 aactgtgctg attatagtga caatccacaa ttattattat ctttgttatt tggatataaa
     8221 aaaggtgcat ttacaggtgc tgaatatgat acaccaggtc ttgtagaaga agctgatgaa
     8281 ggagttttat ttttggatga aatacataga ttgcctccaa aaggccaaga aatactattt
     8341 tctattttag ataggggaaa gtttaggaga ttaggtgaaa ctaatgctga aagaaaagta
     8401 agtattatgt tgattggggc aactactgaa aatgtagaga ctaatttatt attaacattc
     8461 agaagaagga taccaatgct aataactttg ccaccattac atgatagatt actaaaagag
     8521 aaaatagacc ttatatacaa tatctttcaa caagagtgta atagaataaa tgcaaaaata
     8581 tttgtagata aaaatgtgat tgaaatactt gcactaaaaa agtttagtgg aaatatagga
     8641 caattacaaa atatgattca ggttctttgt gcaagagctt ttatgaagtt tataaattcc
     8701 aaagatgaag atagtgaatc gatagtagta gtagatataa atgaagtttt aaagcttaaa
     8761 gattctttta aagatgctgc ctttcaagag attgaatata cagagataag aaaatatttg
     8821 aaaaatgcta tatttatacc atttaattta gaagaatcat cagtaaaagg aagtctttta
     8881 agtgatgaat atatattacc agaggacata tacaaaacaa ttgaaaagaa atattatgac
     8941 ctaaaatccc tagacattga agatatagat atagagaata ttttatggac ttttatttta
     9001 aatagatttg ctgacttaaa gtttaatctc gatagtaatg aagaaatttt ttcaatgagt
     9061 gatttaaata gttttgtaag tgatagttta gttaaactgg taaaagaact tatgaatgat
     9121 atagttgaaa aaaatatgaa tttagaagta aataaaaata tgttcaaata tctagcaata
     9181 cacttagaag aagctataaa aagaattaag ctaaatcaaa agataataaa tgtaaattta
     9241 gaaaaaataa agattgactt ttcaaaagaa tatgaaatta gtaaaagttt tgcaataaaa
     9301 attgaggaat caattggtat taagattcca gatgatgaaa ttggatttat aacactctac
     9361 ataaagtctg ctctaaaaaa tgaagtgaaa aaagataaag tagggttaat aataatttct
     9421 catggacgta tagctacaga gactgtaaat gtagttaaag aacttttagg tgtaaagttt
     9481 cctgtggcta tagatatgcc tttagatgaa aaaccaataa atatatacaa taaggcagta
     9541 gaactgtcaa aaataataga ccaagggaaa ggcatattgt ttttggtgga tattggttca
     9601 cttacaaaca taggtcaaat agtaaataag agaaccaata taagcactaa aactattgat
     9661 agagtagacc tcctaatggc actagaagca actagaaaag tttctatggg agaggaagaa
     9721 cttgatgaaa tatttttttc tttgctaaaa gataggatgg gatataacta tgaattagaa
     9781 aaacacatag ataaatcaaa tgcaattgta acattatgtc ttactggaga aggaactgct
     9841 aagtatataa gtaaaacgtt ggaagaaaag tatgataaca ctaaatgtta tcaaatgagt
     9901 gcattggatg aaaatttatt ttgtaaaatt gaaaatctaa aagagacaaa caatatatta
     9961 gctattgttg ggacaataaa ccctaagata ccaggtataa attttatacc ctataataaa
    10021 gaacttttca aaaatttgga tatctatcta tcaaaatcaa aacaacaaga tgacagacca
    10081 gttaaatcgt atgaaagaat gcttgatgag gatttagtga tttttgaacc agatatatat
    10141 tttaaaaagg atttattaga atatgtatgt tcaattctta taaataaaga atatgtagaa
    10201 aaagattact tagattctgt tttacataga gaagaaatgc ttccaacata ttctaaagga
    10261 gctataggtg tgcctcatgg agattcttct actgtaaata gtacaaggtt tgtttttgtt
    10321 aaactaaaga atcctattga ttggggtgta ggtaatgtaa atttcatatt tatgccagtt
    10381 tttcaggcta atgataaaga gattgttaaa aatgttcttg agatactaaa agatgcagaa
    10441 tttatgaaca atgccaataa gtgttttgat aaagatgctt ttagaaggat tatatttgat
    10501 aaatttaaac atctgtagcc atagcaaaaa aattgagagg agttagagta atgaatgtag
    10561 atttaatatc attgggttta gaatctaatg attatgaaag tgttattgaa gaattaggaa
    10621 gtataatgtg taaaaaagaa tatgtaaaag aaacatacat aaatgctgtc ttggaaagag
    10681 aaaggacatt accaacaggt ctagatattg gagaaatgtg tgttgctatt ccacatacag
    10741 attcaaagca tgtaaatgag tctaatgtag ctgtagggat attgaaaaat cctgtaaagt
    10801 ttaattcaat gatagaccca aaagatagac ttgatgtaga acttgtattt ttattagcag
    10861 taaagaaccc agattcacaa gtaaaactgt taaaggattt aatgtcagtt tttcaaaata
    10921 taaagttatt aaaaaatata aaaaacgcat caacaaaaga agaagtagct aaattattgg
    10981 attttataga aatttaatgt aggagaattt tataaataaa aaattaaaat ttaaacaatc
    11041 aaagtaagta ttattttttt aataggttta atcttatata aaataaaaat ttagagtgaa
    11101 aaattaggag gaaattatta tgaaacaatt taatgtatta tcagtttgtg gttcagggac
    11161 agttacatca tctatggtag caggtaaatt aaaagaagta ctaggtaata aggggatttc
    11221 tattactaca acagaagcta gaccaacaga agcactaaat ttagcacaat caggaagatt
    11281 tgactttata acatttacga gtccacttca aacaggcgat tatggaatac caactataaa
    11341 tgcatttgct tgcctaactg gtatagggga agaggcattc tttgaagaag ttttgactgt
    11401 tattgaaggg atagataagt aagttttgga gacgtgaatt tatagtattt aaatcaatga
    11461 tttagatact ataataagct aatattaact aaatatttgt ggaaaaaaca ttttatatgt
    11521 tgtcataaat taaaaaatta taaaaatagt cacttaagta ttaaattaag cttataaata
    11581 tttataagta ggagggaaaa agatgttaga atttatgaaa tctataatcg atacatttgg
    11641 ttctgcaatt atagttccaa ttataatatt cataatagca aagatattta aagtaactac
    11701 taagaaatcg tttctttctg ctgtgtatgc aggagttgct ttacaaggtt ttacactgat
    11761 tttaaattca tttacaccaa taataacacc agttatcaat aggatggttg agagtacagg
    11821 agtaaaccta ccagtttttg atgttggttg gcaagcaaca tcattggttg cattttcaac
    11881 aagtgcaggt atgatatatt taggtcttgg tatattatta caaacaattt tatttttgat
    11941 aaaatggaca gacgtgtttc aaccatcaga tttatggaat aactattctt atatggtatg
    12001 gggagcaatg gttataggag taacaggaaa ttttccttta ggtatagcat gtatggtttt
    12061 attaaaccta tatagtttat taatatcaga gttagttgca aagagatggt ctagttacta
    12121 tagatatcct aattgtacaa taatagctat gcataatgtt gaagcttctg tgtttgcagt
    12181 atttgcagac cctatataca ataaattggg attaaataaa ataaaattaa atccaaaaga
    12241 attagagaag aaattagggt ttttaggtga accaattact ttaggtcttt ttttaggtat
    12301 gtttataggt atattaggta atatgacgag aataaatact atggaagctt ggggagaaat
    12361 aatgaaagta ggtatatcta caagtgcagt gatggcaatt ttccctaaag tagcaagtat
    12421 gtttgctcaa gcatttgcac ctataacaga agcagctaga aagattatgc aaaaagctgg
    12481 aaaccgtgag tggtacatag cagtaaatga tgctgttggg tatggtgaac ctgcaacttt
    12541 aatatcaggt cttatattaa ttccaataat gcttgtaata gctatggtac ttcctggtaa
    12601 taaggtatta ccagttgtgg atttattggc aattccatat atggttcaag gtttagttgc
    12661 catacataat ggtaatatac caaaagtatt ggtttcaggt ataatttggt ttggattagg
    12721 attatatgtt tgtacatcta cagcaccatt atttacagat atggctacaa atataggtgt
    12781 agctatacct gcaggagcaa tgttaataac aagctttaat atactaggaa aacctttgat
    12841 gggattagtt ttctttgcat ttctaagtgc aaatccaatt tatataggtc tttcagttgt
    12901 aatttacttt gttctatggg cattgtttag aaagaacaaa acaagtattc ttgattattt
    12961 agaaaaacaa gcacttaaaa atgtagaaga agagcctgta gcagtttaag tttataaaaa
    13021 tgaaataaga ataattaaag gagtttataa ttgattttat ataagtatca gataatacct
    13081 gagaaatatt taaatgaaat atagacttct ttttatttga taatatttgt aattataatg
    13141 cattgtatct ttatagttga gttttctgta taacctgcta tttaaaacat actcttttat
    13201 atttatacag taatgtggtt taattttata aaatgtaatc tctactggta aaagactttg
    13261 tatataaggc atttgcctga ataaaccatt tatcttttgt atggcataga tattgctata
    13321 tagaagtata tagcatgaaa ggagatattt atatgggaga taaaatgaga gcatcagtat
    13381 tgtatagtgt aggtgatgtc agatatgaaa tggtagatat accagaaata acagatacgc
    13441 aagtgcttgt aaatgtaaaa tatgttggga tatgtggttc agatttacct aggtctatgg
    13501 taagtggttt gagtggaaat acaaaatatc cattgatttt aggacatgaa ttttctggcg
    13561 aagttgttaa aattggagaa aaagtaaagc atattaatgt aggagataga gtagctgttg
    13621 caccattagt accttgtgga aaatgtgatt attgtaatga aggtaatttt ggcttgtgtg
    13681 atgattacaa tataattgga acaagagtaa atggagcgtt tgcagaatat gtaagagttc
    13741 cagaagaaca tattttaaaa ctaccagata cattggatta tgaaactgct gccggaattg
    13801 aaccagcaac tatagcttat catgggattt caaagtctaa tattagagta ggagatagtg
    13861 tagttgtgtt aggatgtggt ccaataggtc agtttgttat acaatgggca aaggtgtttg
    13921 gtgcatctaa gattatagca gttgatattt ttgatgaaaa attagaactt tctaaacttc
    13981 taggagcaaa ctatatactt aactcaaaag aagtaaatgt aatcaaagaa ataaaaaaaa
    14041 taacaaatgg tggtgctgat gttgtaatag agactgctgg tagccgattt acacaagaac
    14101 aatctctatt tgtagcaaaa aagaggggaa atatagtgtt tgtaggtata tcacacacag
    14161 aattaccact tagtgcagac gctactgagt gtatattacg tggagaactt accttgaaag
    14221 gttcttggaa ttcatataca agcccttatc ctggaagagc atggactgca acattggatt
    14281 ttatgggaaa aggtgacatt atttttaaac ccatgatttc acataaaata ggtcttaatg
    14341 aagtgggtga ctttttaagt aaaatgagca agagagaaat taatttcaat aaaatcctag
    14401 tagaaatata aaaatttaat aaaaaattct aaaagcaatt aaattttata actatgacat
    14461 taaataaaat aaaaataatt ttcttaaata aaatatagga ggaagaaaag tgttagaaaa
    14521 tttaaaagaa gaagtattaa aagcaaactt agaattacct aagaaaaagc taattactta
    14581 tacttgggga aatgtaagtg gaatagatag agaaaaagga ttagtggtga taaagccaag
    14641 tggtgtagag tatagtaata tgactgtaga agatatggtt gtagttgatt tagagggaaa
    14701 tatagttgat ggcaaattaa gaccttcttc agataccccg actcatctag ttttatataa
    14761 agagtttgaa gaattggggg gaatagtaca tacacattca agttgggcaa caatttgggc
    14821 tcaaatagga aaatctatac catcttgtgg tactactcat gcagattatt tttatggaag
    14881 tataccatgt accagaaaaa tgactagaga agaaatttgc aatgaatacg aaaaggaaac
    14941 tggaaatgta atcattgaag aatttatggg aagaaatcca attcattgtc caggaataat
    15001 tgtaaatgac cacggacctt ttacatgggg aaaagatgca aatgaggctg tacacaatgc
    15061 agtagtttta gaagaagtcg caaagatggc ttattataca gagttaatga gtcctgataa
    15121 tattatggat aaggttctta tgaataagca tttttcaaga aaacatggaa aaaatgcata
    15181 ctatggtcaa aaatgattat ctggaggtga attatgctag actctttaaa aggtaaaatt
    15241 gtggaaatag caattaaagc agagcattat ggtttatgta aagaaaagac tggtaatttt
    15301 agtattagag ataaggatac tggatatata cttataaccc catcaggtgt aggaagagaa
    15361 gaattaaaag ttgaacatat ttgtgtattg gatttggatg gaaatgtaat agaagttgag
    15421 aaaggtatta agccaagtag tgaggtattg atgcatattg aaatatacaa aacaagagaa
    15481 aatataaatg ccatatcaca tactcattct ttatatgcaa cagcattttc agtagcaaag
    15541 aaaccaatta taccaattgt atatgaatca gttaattatg gcggttatgt agatattgct
    15601 ccatatgaaa gaccaggaac tttaaaatta gctaagagtg tttcaaaatt actgatagat
    15661 acagatgcat gcttattaga aagacatggg ttgacaactg taggtaatga tttagacgaa
    15721 gcattattaa aatcaagata tgtggaagag gtagcagaaa tatactatag gtctttagta
    15781 ttaaatcaat ttaaagaact tgaagtagta gatgttgatg agtttaaatc ttggaaatac
    15841 ccagaagaaa taaagtttta atctaaataa ttttcattga agtggttaat taaaatatat
    15901 gtattattta tatcaattac tatgttttat tatgttaatt accgtatttt gcaaatatat
    15961 ttattacatt ttttcaaaaa aatatagaat ccgaaagcca tataaaattg acttgggatt
    16021 ctatactttt tatttatgca tttttcttta tctgattttc tggagaattt atatgttcaa
    16081 aatatttttt agtttcagat actattatag gtgataatgc aagtaaagct ataaggttag
    16141 gtattgccat aagcccattt acaatatcag caattatcca aacaacttct aactttaaga
    16201 aagcacctaa taacaccata gccacaaata cagttctata cccattcatg ccttttacac
    16261 caaacagaaa ttcaaaacat ctttctccat aataactcca tcccaaaatt gtagtaaatg
    16321 caaataatgt taaactaact gtaagtaata ttggtccaaa tgtaggaatt gctccgttaa
    16381 aagctgcttg tgtcataaca gctccattta ggtctgaaca ccacactcct gaaataacaa
    16441 gagagaatcc agtaagagta cagattataa tagtatctat aaaagtacct gtcatagata
    16501 taagaccttg ttccgctggc cattcagttt tagcagcagc agcagctatt ggagcactac
    16561 ctagaccaga ctcatttgaa aatacaccac gagcgatacc atttctaatt gccagtgaca
    16621 tagttgctcc taaaaaacca ccagtagcag cagtaggtgt aaatgcactt ttaattatta
    16681 acataaaagt ttcagggatt ttattccaaa aagatattaa aatgattcca cagattacaa
    16741 catatataac tgccataaaa ggtactattt tagtagctac attagatata cttttaagtc
    16801 ctccaaatac aaccaaagct acaaatactg ctaatacaac ccctgtaatt ctagggtcta
    16861 tcccgaaact attatttatt gcatctgtta ttgaatttac ttgagtaaaa gtaccgattc
    16921 ctaaaagtgc aactagtata ccacttatag cgaaaaatat tgcaagaggt ctccattttt
    16981 ctcccatacc atttactatg taatacattg gcccaccaga cacttggcca tttttatctt
    17041 tagttctgta ttttattgca agaacacctt ctgaatactt agtagccatt ccaaagaatg
    17101 ctgctatcca catccaaaaa agtgcaccag gtccaccagc tttgatagct gtagcaacac
    17161 caacaatatt accagtacca actgtagcag ctagagcagt acataaggct gcaaaacttg
    17221 aaacatcacc actacctttg ttttcagcag agaatattaa cttaagagct cttggcagtt
    17281 tagttatttg aacaactcca agcttaacag taagtagtat accagtacca actaatagta
    17341 ccagtagggg tggtccccaa ataaaagcat caactttgtt taaaatatca attagattca
    17401 taaaaacact ccctcatttt ttttgcttta cataatcaat agaattttta atgaagtaat
    17461 tgacaggaaa taagtataat ttattaataa tttctagtgt aataaaaaaa gctaaatcta
    17521 aaaaagattc agctctggat gtacactaat tagcaaccaa aataaaagta ttagtaaata
    17581 ctgtttttgg tagataatcc ctgtcctttt gcctgagaga ttgagcataa aagccttgcc
    17641 ccttcggcgc ccataatggg tctctccaga gttccgtaaa ttttacagtc ctacttttaa
    17701 aagtacctga gagtgttact ccttcggtgt gctatatagc aactcttcta taaaatctta
    17761 accggatatt gatttgtcat agcttattgt attaataata ttaatataag tttttatgtt
    17821 ttgtcaacta aaaaattaaa aaacaaagaa aaaatataaa aatgtaagcc tatttacgaa
    17881 ttataaactt agaaagatga gtaatatacg tgaaaatgaa ttttgtaata cacatacaaa
    17941 tgaagtgtaa aataagttct aaataaaaaa ataataaaat cttaaataat taattagata
    18001 tgattaaatt atggtaatat tatagttacg atattaagaa aagttctatt tagttagatt
    18061 ttaagcgtaa tatgagatta tgtatagaga aaggatgtat ttttagatgg gaaaaatatt
    18121 agtgctagca gaaaaacctt ctgttggaag agatatagca aaagttttag ggtcaaaaaa
    18181 tgaaaaaaat ggttatatag aggggccaaa gtatgtgata acttgggcac taggtcattt
    18241 ggttacactt gctgaccccg aaagttatgg agaaagatat aaatcatgga gcttagaaga
    18301 cctgccaata cttcccaagc accttaagac tgttgtaata aaaaagagtg gaaagcagtt
    18361 taatacagtt aaatcgcaaa tgaatagaaa tgatatagat gaaatagtta tagcaacaga
    18421 tgcaggtcgt gaaggagaac ttgtagcaag gtggataata gaaaaatcac aggtaaaaaa
    18481 aactataaaa cgtctttgga tttcttcaag tacagacaaa gcaataaaag acggatttgc
    18541 aaaattaaga agtggaaaag aatatgaaaa tttgtattac tcagcaatag cacgagcaga
    18601 agctgattgg attattggaa taaatgcaac cagagctttg acaacaaagt acaatgcaca
    18661 gctttcttgt ggaagagtac aaactcctac tcttgcaatg ttgcttaaaa gagaagaaga
    18721 aataagaaat ttcaaaccta aagagtatta tggattagaa ttaattgcta caaagggaaa
    18781 ttcagatata aaatttattt ggaatgataa aaacaataat tcttcaactt ttagtaaaga
    18841 aaagatagaa tccacattaa aaaaagtaaa aggtgtagac ataaaaatat ctgaagtaaa
    18901 caagtcatat aagaaaaaat attcacctgc actctatgat ttaacagaat tacaaagaga
    18961 tgcaaataaa atgtttggat tttcagcaaa agaaacttta tctataatgc aaaaactgta
    19021 tgaacatcat aaagtactta cttatcctag aactgattct agatatttaa catcagatat
    19081 tgttggaaca ttaaaagata gaataaaagc tgtaaatatt agtgattatt caaagacttg
    19141 taataaatta ttaaaaagca atattatagc taataaatcg tttgttgaca attcaaaggt
    19201 aacagaccat catgcaataa taccaacaga acagaaggta ttcatgagtg attttagtga
    19261 caaagagcga aaaatatata atcttgtagt aaaaagattt ttagcagtac tatctcctcc
    19321 ttttgaatat gagcagacaa ctataagtgc aactgtagaa ggagaaatat ttaaggctaa
    19381 gggaaacaaa gtaataaatt taggttggaa agaaagttat aaagatattg aagatgaaga
    19441 tgagtatgaa agtataccaa atattttaga aaaagatgta cttaaggtca agggattaaa
    19501 gataacttca ggaaaaacaa atccaccacc atacttaaat gagggaacat tattgactgc
    19561 tatggaaaag aatggtctag ggacagttgc tacaagagca gatataatag aaaaattatt
    19621 caattcattt ttaatggaaa agaaaaataa agatatatat ataacatcaa aaggaagaca
    19681 attattagat ttagttccag tagacttaaa gacaccagaa cttacagctt catgggaaaa
    19741 gaaattattg gatatttcaa agggaaaact tagtaaaaat acattcataa atgacatgaa
    19801 agattattca aaagatattg tactagaagt aaaaaatagt gataataaat ttaaacatga
    19861 taacttaact aaaaatagat gtccagaatg tggtaaattt atgttagagg taaatggtaa
    19921 aaaaggtaag atgttgattt gtgaagatag agagtgtaat actagaaaaa ctatatcaca
    19981 agtaaccaat tcaagatgtc ctaactgcca taaaaaatta gaacttcgtg gagaaggtga
    20041 ggggagaatc ttcacttgta gttgcggata cagagaaaag ttgtcgtctt tcaacaagag
    20101 aaagcaagaa gaaaaacaga aggcatcaaa aaaagatgtt aatcaatatt taaaaaatca
    20161 aaataaaaat caagagacat ttaataatcc atttgcagaa gcattagcta agctaaaaaa
    20221 caaataattt taaagttatt attatacaca attataactt taactgtata ttagttatat
    20281 gtactgatat aatatagtgt atagtttagg catgaaagta gggctgttaa atggatttaa
    20341 gtggcatatt taaatactat tgtaaagagt gtgaaaatac atggaataat tcgagtgttg
    20401 aattatttga gaatatagaa acgtatagta aagattcaca aaaaaagagg gaaaaagaat
    20461 tagataaatt gctaaataca atatcagttc atttagagag gtatccaagt gatgctgtat
    20521 tgagaaaaat gtgggtaaaa aagggcgagg ttttcttaca aaagacattg gaaaaagaaa
    20581 atatttttaa gttagaaaaa atggatgtag aggatagaaa aaaattttta gatataacaa
    20641 aacagtttat tagagatgct agaaaatttg atgatgattt acctataggt gatattatgc
    20701 aagctatgag gaatgtatgg atttcaaatg cattacaatt attatttggt aaagaagtat
    20761 attattcaaa agctaacttt gcatatagta tgttatatcc atatactgat aattatttag
    20821 acaatacaaa tatagataaa aatgataaga ttttatttaa taactggtta gaaaaaaggc
    20881 tcctgggaga acacattaaa tctaaggatt atcatgaaag taaagtatct aaaatgatag
    20941 attatattga aagtgtatac cctagagaaa agtttacaga agtttatgaa tcgttattat
    21001 taatatttaa aagtcaagta aatagtttaa aacaacatgg taaggaaaat catttgtgta
    21061 aagaagattt attatccatt tctattgaaa aaggaggttc atccgtttta gtagatggat
    21121 atttaataag tggattgatg acaaaggaag aaatagagtt ttgtatagga tatggatttt
    21181 tattacaaat atctgatgat ttacaagata taaaagaaga tttaaaatac aaccataaaa
    21241 ctattattac agagatgtca aaagagggta ctttagataa agttgtaaat aaactaataa
    21301 attttactat tgagttaata gatagtttta aaattaataa taaaaataaa tctgtaataa
    21361 ctatgataaa gaatgattgc ttaatgttaa ttttattttc tgtagtttat aatgctgaat
    21421 ttttttctgt aggatatata aaagaagtag agaaatttat tccatataca atagattatt
    21481 cattagagat tgaagaaaaa ataaaagaaa aatttaaaaa tatagatgtt ttaaataatg
    21541 aaaatgaata taaagaaatg attgatatta tttgtgcaga gtagaaaaaa agggagtgtc
    21601 ttagaataga ctttttagtt tatgaggcac tctttttgta taaaatcaaa tgtattttca
    21661 tcattttaat aattaaaaag agcttatccc tatttgagaa agcccttttt aatttaataa
    21721 aaatgtcaag gttaaactat ttaaatgcag taataatccc attatcatgt aagtttttta
    21781 tgattgtagc tgcgataggg aatccaaata atccagctat tccaaatagt tgagctccaa
    21841 caaacataca cattaatgtt attaaaggat ataggccaat ttggtgtcct actacctttg
    21901 gttctaatat ttgtctaaca acggttataa ttatgtatag tattaaaagt cctattgcta
    21961 aattaatatt tccattaatg agtgagataa tcatccaagg tgtcaaaata cttcctgtac
    22021 ccaagactgg caaaatatca acaagtgcta tgatgacaga tatagtaaat gcattttcaa
    22081 cctttaggat agtaagtcca ataagtagtt ctatgaatgt cactgatagt agtattgcat
    22141 aggcttttag tagttttaaa agagcattaa tactgctatt ttttatattt attatcataa
    22201 attgactttt ttctggaaat tgttttaaga taaagtttac tattttttga tagtcaaaag
    22261 taaagaaaaa agaagcaatt aaagtaataa taagttttaa cataaatgca ggtgttttac
    22321 ttgcaatact agcaatagca ttgagtgcat ttgttgaaat agaagctact aaagacatca
    22381 tagattgact tatattatcc caaccaagta ttaatgctag gttaacatta ggtgtactag
    22441 catctatctt tgaaaataaa gtatttagcg ttggttgtat acttgattta tatatttcag
    22501 gaagtctaaa aaatacatca cttacacttg caaatatctt cacaccaaag ctaactaata
    22561 aaaatataca caacccataa aaaattaaaa gtattactat agaaataagt gtacgattta
    22621 aatttgtatt atttgtaata agtcttataa taggtcttaa gatggatgca atgataaaac
    22681 tgattacaaa aggcattaat aatggcaata agaattttaa tacaatatat acaatacttg
    22741 ttattaaaac tacatatgta aatttaatta taaaattctt ttgcttttga atatccataa
    22801 tataaaacct ccaatgtatt tgatattatt ctaaatatgg tcttttttac agaaaatata
    22861 ctactgtata ttgattataa actataaagc ttatgtaaag taaataaatt atgtaaaatt
    22921 taggttaatt ttgtataaag agatactttt atattttaca attaataacc aaatactaaa
    22981 aaatatttta aataaagaac ttaaaattgt gataaaatta atttatagat attaaaaaaa
    23041 gtataatata tggtttttaa gaggtgctaa gatgaaaaat tttagagatt taggtgggaa
    23101 caaaactgag gatggcagaa ctgtaaagaa gggattattt tataggtctg caaaattatc
    23161 aaatctaagt gaaaatgata taaagatttt aaaagagtta aatattaaat atatatttga
    23221 ttatagaagt gatgaagaag ctcgtaaaca tccatcaaca ataatatcaa atataaaaaa
    23281 tataagaata ccagcaatga gagagctaga agaatcagga ggaagttttg gttctattga
    23341 agatatgatt gatggattgt ttgaaaaaga tggtgcattt aatatgttaa ataatagtta
    23401 ctataatttg cctataaata atccgtcata taaaaaatta gtagaactta taagagacta
    23461 ttctaattta ccaatattaa atcattgtac agctggtaag gatagaactg gtgtggggag
    23521 tgctataatt cttatgattt taggggtatc gagagagaat ataatgaaag attatctaaa
    23581 aagtaatgat tttgcagata aagagataga aagatttata gaatataagc ctaaatttaa
    23641 ggacattcca aaagaaaatt tgaaatatat atttggtgta aatgaagagt atatgaaaac
    23701 tgcatttaga agaattgatg aagaatacat tagtgtagaa gcatatttat atggagaatt
    23761 taatttaaat aaagaagaaa taagaaaatt aagaaatcaa tatctagagt aaaaggtgag
    23821 tttatgagtt taaatataac agcaagacag ataaatataa taaaaatatt gttaaattca
    23881 aaagatacaa ttagtggtat tgccttaagt caagagatag gatgttcttc aaaaactata
    23941 caaaatgaaa taaaggatat taataaacaa cttagaaatg gtaaaatttt gtcaataaga
    24001 ggagttggtt ataaactaga aggaagtttt gatgaggtaa atctaaatag caatctctat
    24061 gatgatgtag atagagtaga atatataatc agaaaaatat taaccttaag taatgaagaa
    24121 aaaaatacta taaaacttga agatttagcc gattctatgt atgtaagtgt ttcaacagta
    24181 aaaaatgatt taaaagaagt aaaaagaatg ttggaaaagt attcaatatc agttaaatct
    24241 aagcataaac aaggtatatg tgttttagaa agtgaagata agatattaaa ttgtatagtt
    24301 gacttaagca ataagagaga taatcaattg agtttaaatg attttttaaa tgatgatata
    24361 aaggaaagaa aactattaat aaaaaaatta ttattagaca tattaaatga aaatggatta
    24421 gtattaactg atatagaatt taagagtgtg ttaaataata tattagttat gttaagcaga
    24481 cataagtata atgaaaaaga atttatagag aattatatag aaaattatag gagcaaaaga
    24541 agctcaattt tagaaaatga acaaaacaaa gaaattataa taaattcaat aaaaacattt
    24601 tgtaaaaatt taaaattagc tacatcaata gatataagta atgataaggt atttgaagag
    24661 tttttatata agcatattag tagtttatgt aaaaaaataa agttaggtat aaatcaatca
    24721 cctataatgg ctcaagatat aaaaataaaa tatccatttg cttttgaact agctaatata
    24781 gctaaaaaga caatagaaaa agatttgaag atagaaatta atgaagatga agttgcaaac
    24841 atagctctac atataggagg agcagttgaa agagcatctt ataataataa agataaggtt
    24901 ttcaaaacta taatagtttg tacttcaggc ataggcacat caatgttaat aaaagctaaa
    24961 ttagagaata tatttaagga aaaattagaa atactaaaga ttattccttc atacttagtt
    25021 gaatatataa aagttataga tgttgatttt gtaatatcta ctgttcctat tgaagtagga
    25081 gatgtgccag taataaatat atctccaatt ttaagtgaga aagaaattag attaattgaa
    25141 aaatatatag aaacaggaaa tatataccta gatttagata taaaaagcct gtttgatagt
    25201 gaattgtttt ttactgattt ggtgtttaaa aggaaagaag aagttataga ttttatggca
    25261 agtaaacttg tacaaaaggg ctatatagat gaggatatga aaaaatctta ctttgagaga
    25321 gaaaaaatag caacaacaga aatagggaat atggtaacta taccacatgg agctattgga
    25381 aaaatttttg ataataaaat agcaattggt atattaaaga agcctatttc ttgggaaata
    25441 tcagatgttc gtttaataat aatgttagcc cttgataaag ataaaatatt agattatgaa
    25501 gtaatatttt caaaaatata taaaagagta gattctatag caaaagttat aagtatatgt
    25561 gaaaatagta gttttgaaaa atttgtaaat ttatttaatt agatgggtag tattgtatat
    25621 acacctgtaa atttggataa tataaaacta aataagataa aaacgataaa tttctctttt
    25681 aattaagaga aattttttga ctttcaagga tattaatata aagttaagat ataaacataa
    25741 agcaaataaa taaaaaagag gtggcaatat gatttataca gtgacattaa atccatcaat
    25801 agattatata gttaagctag atgagctaaa gacaggaagt acaaatcgtg taaaagaaga
    25861 atacgtttat ccaggaggaa aaggtataaa tgtatctcgc atacttaagg aacttggaaa
    25921 tgataataca tctttaggtt ttataagtgg ttttactgga gaatatataa taagaacatt
    25981 ggaagaaaaa gaattgaaga cagattttat aaagataaaa agtggttttt caagaataaa
    26041 tgtaaaaata aaggcgttaa aggaaactga gattaatgga caaggtccaa atatagatga
    26101 tgaagacatt gatactttat ataaaaaact tgataagcta aatcaagatg atattttaat
    26161 attggcagga agtataccat ctactttaga tgaaaaacta tacgaaaata taatggctag
    26221 attagaaaaa aagaatataa aagttgtagt agatgcaact aaaaatcttc ttcttaatgt
    26281 tttaaaatac aaaccttttc taattaagcc taataacgac gaattagaag aactatttgg
    26341 tgttaaatta aattcaattg aagacatggt aaaatatgct agaaggttaa aggaaatggg
    26401 agctataaat gtcttggtat caatgggaaa agatggagca ttgttgatta cagaggaaga
    26461 tgaagtgctt ataagtgatg ttccaaaagg aaaagtaaaa aattctgtag gagcaggtga
    26521 ttcaatggta gcaggattta tatctgggta tttgaatact gggaaatatg attatgctct
    26581 taaattggga gcagcttcag gtagtgcaac tgctttttca tatgacttag ccaaaagaga
    26641 atacattgat aaattggtaa atgaaatatc tgtaaagaag ttttaaaata ttaaaaaata
    26701 tatagaaagg taattaatat ttatgaaaat tatagattta ttagatgaaa agtcaattaa
    26761 gttaaattta aattcaaaaa ccaaatcaga agcaatagaa gaattagtag atttggtcgc
    26821 aaatagtgga aatttaaatg ataaagaaaa ctacaaaaaa gcaattttgg ctcgtgaaga
    26881 aatgagtaca acaggaattg gagaaggtgt agctatacct catgctaaaa attcttcagt
    26941 tactaaagct tgtatagcag cagcagtgtc aaaagaaggt atagattatg aatcgtttga
    27001 cggaagttta tcaaatctat tttttatgat agctgcacca gacggagcta acaatactca
    27061 tcttgaagta ttatcaagat tatctacaat acttatggat gaagacttta gaaataaact
    27121 tataaattca agctctgaaa aagaatttaa ggaaataata gataaaaagg aaagagaaaa
    27181 attcagtgaa gaatatcaag atgagaaagt ggctgaaaaa aatataatag atactaaaga
    27241 gaatgatgct aataagtatc ctaaggtttt agcagtaaca gcatgtccaa ctggaatagc
    27301 acatacattt atggcagctg aaagtttaaa taaaatggca gaaaacaaag gtgtatcaat
    27361 aaaagtagaa actaatggtt cagcaggtgt taaaaataag cttacaaaag atgaaataga
    27421 aaatgcaaca tgtataatag tggcagcaga taaaaatgtt gagatggcta gatttaatgg
    27481 caaaaaagtc ataaaaacta aggtggctga tggtatacac aaagcggaag aacttataga
    27541 caaggctgta aatggagatg ctccaattta ccatggagga gatggaagtc acaacgaatc
    27601 aaatgaagaa agtgaaagtg gatttagaaa agtatacaaa cacttaatga atggtgtatc
    27661 taatatgtta ccatttgtta ttggtggagg gatacttata gcgatagcct ttttacttga
    27721 tgattatact attaaccctt caaactttgg ttcaaataca cctatagcag cattttttaa
    27781 aggaatagga gataaagctt ttggatttat gttacctgta cttgctggat atatagcata
    27841 ctctatatct gatagaccag cgtttgttgt aggatttgta ggaggtgctc tagcaggtga
    27901 tggtggttca ggctttttag gtgcactact tgcaggtttt atagcaggtt atttagtaga
    27961 agggcttaag aaaatattta gtgtactacc agcttcattg gaaggaataa aaccagtatt
    28021 gttatatcct ttacttggaa cacttttgat gggaattata atgacatttt tagtaatacc
    28081 tccagttaca gcaattaata atgcaatggt tggattttta aatggtttag gtggaacttc
    28141 aaaaatagtt ttaggattag tattaggtgg aatgatggct gtggatatgg gtggacctgt
    28201 aaataaagca gcatatgtat ttggagttgc atcacttgaa tcagggcagt ttgaaataat
    28261 ggcagcagta atggctggtg gtatggttcc gccacttgct atagctcttg caactacatt
    28321 ttttaagaat agatttacta aagaagaaag agattctggt aaagttaatt atgtaatggg
    28381 attatcattt gttactgaag gagctatacc ttttgcagct ggagacccac ttcatgttat
    28441 accagcttgt gttggaggtt cagcagtggc aggagcactt agtatgttgt tcaacgcagc
    28501 tcttcgtgca cctcatggag gagtatttgt tataccagtg gttacccatc catttgcata
    28561 tatattagct atagctgtag gtgctttggt tggaatgatg cttttagcat tacttaaaaa
    28621 accattaaat caagaagtat agcaattaaa ggatattcaa gaacttaaaa tatgatgttt
    28681 tataaaaatt aatatattgc tgaattattt taaaataagg attttatgag gctgttatat
    28741 aacgatttgt aattgttatc ttgacagtct tttattttta tataagtaaa ataagtgtgt
    28801 tttgctttat
//
LOCUS       DAMJWS010000050        28649 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMJWS010000050 DAMJWS010000000
VERSION     DAMJWS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.050"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            40..486
                     /locus_tag="PC399_002250"
     CDS             40..486
                     /locus_tag="PC399_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2593107.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            488..1582
                     /locus_tag="PC399_002251"
     CDS             488..1582
                     /locus_tag="PC399_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF2593108.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADRNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1670..2200
                     /locus_tag="PC399_002252"
     CDS             1670..2200
                     /locus_tag="PC399_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2593109.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(3137..4513)
                     /locus_tag="PC399_002253"
     CDS             complement(3137..4513)
                     /locus_tag="PC399_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593110.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILSNYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4781..5953
                     /locus_tag="PC399_002254"
     CDS             4781..5953
                     /locus_tag="PC399_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2593111.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPAICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            5967..6614
                     /locus_tag="PC399_002255"
     CDS             5967..6614
                     /locus_tag="PC399_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593112.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6616..7125
                     /locus_tag="PC399_002256"
     CDS             6616..7125
                     /locus_tag="PC399_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HDF2593113.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVLVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7489..8601)
                     /locus_tag="PC399_002257"
     CDS             complement(7489..8601)
                     /locus_tag="PC399_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2593114.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            8899..9165
                     /locus_tag="PC399_002258"
     CDS             8899..9165
                     /locus_tag="PC399_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HDF2593115.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            9410..11137
                     /locus_tag="PC399_002259"
     CDS             9410..11137
                     /locus_tag="PC399_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593116.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11137..12927
                     /locus_tag="PC399_002260"
     CDS             11137..12927
                     /locus_tag="PC399_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593117.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13089..13397)
                     /locus_tag="PC399_002261"
     CDS             complement(13089..13397)
                     /locus_tag="PC399_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593118.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(13659..14207)
                     /locus_tag="PC399_002262"
     CDS             complement(13659..14207)
                     /locus_tag="PC399_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593119.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(14310..14981)
                     /locus_tag="PC399_002263"
     CDS             complement(14310..14981)
                     /locus_tag="PC399_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2593120.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            15377..16282
                     /gene="garR"
                     /locus_tag="PC399_002264"
     CDS             15377..16282
                     /gene="garR"
                     /locus_tag="PC399_002264"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF2593121.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            16418..17800
                     /locus_tag="PC399_002265"
     CDS             16418..17800
                     /locus_tag="PC399_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2593122.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            17871..19307
                     /locus_tag="PC399_002266"
     CDS             17871..19307
                     /locus_tag="PC399_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF2593123.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSEKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKILGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(19493..20347)
                     /locus_tag="PC399_002267"
     CDS             complement(19493..20347)
                     /locus_tag="PC399_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593124.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHTIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(20615..20938)
                     /locus_tag="PC399_002268"
     CDS             complement(20615..20938)
                     /locus_tag="PC399_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2593125.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21141..21770
                     /locus_tag="PC399_002269"
     CDS             21141..21770
                     /locus_tag="PC399_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593126.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            22113..22622
                     /gene="ruvC"
                     /locus_tag="PC399_002270"
     CDS             22113..22622
                     /gene="ruvC"
                     /locus_tag="PC399_002270"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2593127.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            22615..23217
                     /gene="ruvA"
                     /locus_tag="PC399_002271"
     CDS             22615..23217
                     /gene="ruvA"
                     /locus_tag="PC399_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF2593128.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            23324..24343
                     /gene="ruvB"
                     /locus_tag="PC399_002272"
     CDS             23324..24343
                     /gene="ruvB"
                     /locus_tag="PC399_002272"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF2593129.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            24661..25686
                     /gene="queA"
                     /locus_tag="PC399_002273"
     CDS             24661..25686
                     /gene="queA"
                     /locus_tag="PC399_002273"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF2593130.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            25698..26183
                     /locus_tag="PC399_002274"
     CDS             25698..26183
                     /locus_tag="PC399_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593131.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            26262..27383
                     /gene="tgt"
                     /locus_tag="PC399_002275"
     CDS             26262..27383
                     /gene="tgt"
                     /locus_tag="PC399_002275"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF2593132.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            27517..27810
                     /gene="yajC"
                     /locus_tag="PC399_002276"
     CDS             27517..27810
                     /gene="yajC"
                     /locus_tag="PC399_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF2593133.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            27868..28230
                     /locus_tag="PC399_002277"
     CDS             27868..28230
                     /locus_tag="PC399_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF2593134.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            28296..28439
                     /gene="scfA"
                     /locus_tag="PC399_002278"
     CDS             28296..28439
                     /gene="scfA"
                     /locus_tag="PC399_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF2593135.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
BASE COUNT        10842 a         3310 c         4869 g         9628 t
ORIGIN      
        1 atagtcaaac atattataat tatagaaggg aaatttaaga tggattttaa aaatttacag
       61 tatgaaagtt tgtcatatat tatttgtaac ttgcaaaaaa actttaaact ttattgtgaa
      121 aaatgtttaa aaccatataa acttacgaat ggtctatatt tctatttaat ttatattaat
      181 aaaaatagga attgttcttt gaatgatgtt tctacagaat ttgaagttga taaagctcat
      241 acaacaagaa ctatatctag gttagaacaa gatggataca tagaaaaaat ccaaaaccct
      301 aatgatagta gagcttttca gttgagagta acagataagg gtgaggaagt attaggggat
      361 ataaaaaaca tattttcaaa gtgggataat cacataaaaa aagagttctc ggatacagaa
      421 tataaggaac tggtaaagaa tttacatgtg gtaaaggata taaaaacagc tgtggaggaa
      481 gaataggatg tattgtaatt tattaaaacc aattaaaatt ggaaatttgg aattaaaaaa
      541 tagagtatct tttgcaccta caagcatggg tcttaaattg gaagaaaaaa ttaaaaagtt
      601 ttctgatatt gctaagtcag gagtagctct gattactcta ggagatgtat ctattagacc
      661 tagttttcat aaggtagcta taagcctatc ggatgaagat ggagtcatga aatacaagaa
      721 aattgtagat gaaatacaca attctggtgc aaaggtatca gcacaactat tttgttcaga
      781 ttatgatgtt aatttaatta aagatactat gaatatggga attacttcac atgatgagat
      841 aaaaaaaata atgaatgacg gagtaaaaga ttacataaca aatatgccca aagaagaaat
      901 taaaaatatt ataaatttat ttaaagtaac agcattgaat gcaaaaaaag caggttttga
      961 catgattcaa attcatggtg ataggcttgt aggaagtttt tcatcaagta tatttaataa
     1021 tagaaatgat gaatatggta gaacgtgtga taatagaagt agatttgcaa gcgaaataat
     1081 tagtagtatc cgagatgaag ttaaggacat tccaatagac tataaatttg caataagaca
     1141 agaaaatcct cattatggaa atgcaggagt tttactttcg gaagttgagt attttgttaa
     1201 aaaatttgaa tccctaggtg taaacagttt tcatgtaaca cttgcaaatc attcaaaatt
     1261 agaagatact ataccaacca ataatcatcc ttactttaaa gaagaaggct gttttttata
     1321 tttagctgat gaagtaaaga aacacacaaa tcttccagta tgtggggttg gaaaattgtc
     1381 tagcccagat tttatagaaa gtattatttc aaataataga gtagatatgg tttctatgtc
     1441 aagacaatta ctagcagata ggaattggtt acagaaagta aaagatggaa gagtagatga
     1501 gattaaaaag tgctgttatt gtaataagaa gtgtgcagat gctttacaaa cacgctctca
     1561 gtttggatgt atactagatt agaatatcct tataaaaata aataatttgt taaggttaat
     1621 atatttttgg tgatttagat aagtatttag ataaagagga ggagacatca tgaaaagttt
     1681 agttttatat tcaagtttaa ctggaaatac taaaaagatt gcttatacta tatatgatga
     1741 aattcaagaa gaaaaagaca ttaaagatgt aaatgaatta gttgattata gtattgacta
     1801 tgaaaattat gatatagtat tcttaggcta ttgggtggac aagggaattt gtgataaaaa
     1861 ttcaaaacaa gtgcttgaaa atattcacaa taaaaagata gcactatttg gaacaatggg
     1921 agcaagtaaa aaaggaagtt atggagctag tataatagaa aagatagaat caattatccc
     1981 aaaagataat gaaatattag gttcattcat atgtcaaggt aagatagcag aaggattgaa
     2041 agcaaaatat aaagagatgt taaaactttc tcctgacaac gaacatattc gccaacaatt
     2101 aaataaccat gaggaatctc aaagtcatcc agatgaacaa gaaatatatg aagccagtat
     2161 gtttgcaaaa aatatgatga ttaaagctag tattgtgtag tagttaaaca ataagttcat
     2221 ataagatata gaataaataa tttagtaaaa aagtatagca aataatttgc tatgcttttt
     2281 ttatgaaatc aaaaaaataa taaaaatact tgactgtaaa ccttgtttat acattatttt
     2341 atagaaaata agatagtttt aaatataaaa aaagatatat tattgaaagg agatattatt
     2401 gtatgaaaac ttataaaaga taattagttt gattttaaga tatactaaat atacaatttt
     2461 atagagtgaa aatatttaag actatattat agttataaat taatagaata gagaggtagt
     2521 gtttaaattg caataacagt atttccaaag ttgccacctc aaattcctat ccaatggaat
     2581 aatggtgtag ctaattatgt agataaaaaa tttatttatg cctaccctgt catttgtgtt
     2641 gtattcagaa tccttttacg tccatttata tggagatggt tacaaataca tgctttttat
     2701 agtgacacta ttgcagatta tttaacgaat tttatgtgtt ttgtagcgtt gtctatagaa
     2761 atattctcta tactttttat ttacaatatt gccagacatg ttacaataat tatttttata
     2821 gatatacttg tatttattgc tttactgata ataggtatgt ataaattatt taaaaaatac
     2881 aattaatttt taaaaattaa gtaataagtg atagtgtaaa aagtagccac tgcaaattat
     2941 ggattgtagt ggctatttta ttatttgtat atgaaattat tttgtttttc taataagtta
     3001 cgatatatta tggtttattt cactgataaa aggtttaggt attttaaagt aaaaagttgt
     3061 atattctaat aaaatttaaa cattttgatt ctttaaaatt agagaaaata cattgttctt
     3121 attaaaaaat taaatattaa gaatttaatt tctgcatctt atttagaaaa gataagaaat
     3181 caactttata tatttttttg ggccttcctc tttgattatc gagttttgta tttacaatag
     3241 ttgcaattcc tgctttatat aagatactca atattctatt agcacttcta agagttatgt
     3301 tcatatagtt tgataatatt tctgctgaca cattatttga atttctatca ttaatcatac
     3361 acatgatttt agatacattt gtattagata gaggtataaa agaagcaact ttattggcta
     3421 ttgagctatc ttcaatatta ttagaatttt tatttgaata taaagggcct attgtatcat
     3481 tagtatcatt tactataaaa gtacagtttc cattcaaaga agcagattta ccattagctt
     3541 gacgagcatt ttgctctgca tgaatatttg tatttccaat gccccaacct atatttaagc
     3601 tatatttaat ttcttttaaa tcttctgaaa taaaaaaatc tgtatagtta ttggtcatat
     3661 tgtttaacat ttctttagta gtgtgtatct ctattttcat atcatttttt ttgacctgaa
     3721 ctccataaaa tccatgtctt gatagagaat ttaatatagt gttatatatt tgattttgta
     3781 tctcatcatt tttaaatatc aaatcttcat ttatgtactc tgaactattt actgtaacta
     3841 tacaagttgc agatttattt ttgtctaagt tttgtaatgt tatttcattc accagagatt
     3901 taaatgtatt caggattgat tctgtagatg gaaccatgta aatacaatca tatccaagtt
     3961 tattaagttc acaactaatg atgtaatttc tagtaaaaga aaggttaatc ttattatctc
     4021 gcaataagtc taaatgtatt tccaataatt tatcatttaa ttttttaaag tcatatacag
     4081 gttgagggat attaaaatct ttaattgtat atggacattt attagagtct attaatgaat
     4141 atatatcata ataattgttg tcctctctta aaaaatccat aaatgatttt gtaaagtcaa
     4201 tatctttttg gctatttaat aatttgaata agtatttata aaaatctctt tcatcaagct
     4261 ttaaaaaatt taatggaact tttatgtctt ttatatttat tataagttca ctatatggaa
     4321 ctataccact gcatacaaat ccatcaaaat tatctacttg ctcatagtat atattaggta
     4381 aattattaaa aggattctcc cctttgagct caataaatgt aatttcacaa gtatcattta
     4441 attctttaag agcttgttta aacttatctg acataaaata agatgttgtc aaaaaaccaa
     4501 ttttatattt cataaaacct cctttaaaat ttcgagagta tagtaactaa tcaatcaatt
     4561 tataacctca ttttataata gtagataaaa cgcgtcaatt tattttacaa aaggaaaatt
     4621 taaagaaaaa tcagaaaata taaaaaagac tgaaaaatat gttgactgtc aagaataatt
     4681 gtgctatttt gtaattaagg actagtcttt taagatttcc taaaataaac ttagatttta
     4741 ggaaagaaat acattttatt tgaaaataaa ggagaagatt atgaatacat atttaaatga
     4801 aatttctaag tttgttgatg agaatagaga agaaatagtt tcattatgga aagagattgt
     4861 taatatagaa agctatacac attgtaaaga aagcgtaaat aaattggcta agagattaaa
     4921 gtttgagttt gaaaaagaag gtttagattg cgagttagta gatgttggag ataatggtag
     4981 tacattgata ggtacacttg gttctaatat aaataaaaag cctataattt tttcaggtca
     5041 catggatact gtctttgaaa caggtacatt tggagagaat ccatttaaaa taatagaagg
     5101 aaaagcatat ggcccaggtg ttttagatat gaagggtggt ataataattt ccttatatgt
     5161 tattaaagca ttaaataaaa taggatacaa agaaagacca ataaaaatag tattttctgg
     5221 agatgaagag ataggacata aggattcaac aggtgcagat gttatattga gggaagcaaa
     5281 aggtggtctt tgtgctttta atatggaaac tggattagta gataatagtt tgtgtgtagg
     5341 tagaaaagga agaataggat gtaatataca tgtaaaaggt gtagaaacac acgcaggtaa
     5401 tgattttgaa ggtggaagaa atgctataga agaaatggct aataagatac tacgcataca
     5461 aaaattgact aatttagatg ttggaactac agtaagtgta agtgtgatta agggtggaag
     5521 agtggaaaat tctattccag aagattgtag tataaaagta gaccttagat ttgaaaaagt
     5581 agaagaaatg gagaatgtta aagaacaagt tagagagata tgtaaagaaa cttacataga
     5641 aggaacttct acagaagtta attttataag tgaaatgatg ccatttgaaa ctacagaaga
     5701 tgttatgaga tttcatacat ttgtaaatga agtgtcaaaa gaaaatggat ttggagaatt
     5761 aaatgctaag agacttggag gtagttcaga tgctagttat ttgactattg caaacgttcc
     5821 cgcaatctgt tcatttggtg taagagggga atggaaccac acatcaagag aatatgcagt
     5881 agtagattct atgtttgaga gggtgaaatt aatttctaca gttattttaa acttagataa
     5941 atttgaggta taaaggggga ttttatatgg atgtaacagc tggagataat aaggtaacat
     6001 ggaaagggta tttatcttta tttgtattga tagtattatt ttcaggagtg tttaaagata
     6061 gtcaaggctt ccttaaagca ttagattttg ctaatctgac aggttcattt ggtactattg
     6121 cagatggtgt agactttatg ggtaaaggag gaacgggagc aaaagatggt ttcttatttg
     6181 ctctgacttt gattccaact gtctgttttg ctgtaggtct tatagatgta gttgaatcta
     6241 tgggaggaat gaaagcggca tcaaagatat ttaatccatt gcttagacct ctgcttggta
     6301 tacctggaat agcaggaata gcatttgtat cttcatttac tagttcagat gtagcctcaa
     6361 taatgacgaa agaattattt gaaagtgggg aaataacaga tgatgaaaga actatatttg
     6421 tagcatatca atatgctggt tcagctgtta tactgaacac aataaatact caagctccac
     6481 ttttaccaat agcattattg gcattaggtc caataatcct tatagagata ttctgtaaaa
     6541 tacttggtgc taatctagtt agattaatca taggtgtaag aaataaaaag aataaggcta
     6601 aggaggctgt gtagtatgag tcaacaaaat actggaaatg caattgtaaa tagtaaacct
     6661 aagaaaactg ctgttgagat atttatgggg ggagcaaaaa aaggttttta cataggtgtt
     6721 gaacagatat taccagccat gataatgggt tatgtaatag tgcagttttt acaattaact
     6781 ggtttagttg atattttagg aaatattttt ggaccagtaa tgggtatttt tggactacct
     6841 ggggaatctg ttttagtttt gatagcagca ttctttgcta aggcagcagg agcagctaca
     6901 gctgcaaact tatttgcaca aggtctaatc actgcaccac aagcaaccat attaattatg
     6961 ccatgtatgt taatgggtac tctagttggt cattatgcca gaatagtgct tgtagcaaat
     7021 gccagtacta agtatagaat gttactgttg gcagttccta tatttgatgc aatagtgggg
     7081 atgttaatag taagagtttt actcactgtt atgggattaa tgtaaataac ataaaattat
     7141 aacctaacta taaatgatat ataagaatta atatatacaa ggattaatga gtagtaattg
     7201 atatatagta ataaactaag atagtaatta atgtgtatat taattaaaaa aagagagtat
     7261 ctcaaagtga actttttagt tcatgagata ctctttttgt atgaaatcaa gtatattttc
     7321 ataatttcaa caatgaaaga gaggcttatc tctattttga gacagcctct tcttttaaat
     7381 ttatatttct agaatttatg ttttttattt atccaacatt aacattagtt attagaaata
     7441 attttaagaa aaatatactt tcatgttaat atcagaataa atctgttttt aaaggatgat
     7501 ttgttttgaa ttttcacgaa tatctacttc tatgactttt tctattgtat ctcctctaaa
     7561 acctaataaa ttacttgtca agtttgcagt ggaacaagaa tgattaggta taattcttat
     7621 tctatcacca atttttatat cagtttcacc atttacttta atttttccaa cttcttctga
     7681 aagtgaatca atagtaagtt ctggatgtcc acatacccta ccataaccaa caatactact
     7741 atttccatgt gcacctttat caagtccaag gcacttactt ccacaatcaa ttaaatagta
     7801 tccactgcta ttttttgaga ttacagtgca cacaacagta agagcacaca tatcttcagt
     7861 agcacaacct aaacctatct gtatagcatc atagtataca tagtttccag gtcttacagc
     7921 agttatagtt ttagaagcag cttcagattc aactgttgga gtactaccag aagcgacaat
     7981 ttctatatta aatccattat cagttagagc tttgtatgct ttctccatag aagaatcttc
     8041 ttgaacacaa acatctggaa caccttctgg agaactacaa ccatacactt gacctggatg
     8101 cgaacttata ccaacaatct ttatgttttc tagcttagaa atttcagtag caagttcaac
     8161 cactttctct ggttcaaccc caaaacggtg taagccacaa tctacaataa taagtagctt
     8221 gcactctatt tttgatttag aaaaagcttc actataagcc ttagcacatt ctatattatc
     8281 tgtagcacag attaaattta tttttgatgc catagtcaca attctattta tatttacttc
     8341 tcccatgtaa ggataagcca acataatatc ttcaaaacct gcttttatca gagcttcacc
     8401 ttcatcaagt gttccaacta gaaaaccact agcacctgca tttttttgaa gtctcgctat
     8461 gtaagtactt ttatgtgtct taagcatagg ccaaagttgt ttattattat ttgtacaaat
     8521 ttgttgaatt ttatctatat tcttttctaa ctggtctaaa tctagcaaaa aatttggagt
     8581 agcgacttct gaaattttca tgttatttac cacctttcaa caaaagtaaa ttaactataa
     8641 gtattataat tattatatta gtaatatcgt ctttataata ttaatagctt ataattatat
     8701 tatagaacta gcgttaacag agagcaatat ttatttgtaa taattagaat tttcaataag
     8761 ttagtgtggt tttaaattat ttatataata tatttttaat tttatgcaaa gtttatttaa
     8821 attagtttga ggatttatta atagggtatt attgaatgag taaaaaagtg atgtttacat
     8881 agaataatca cactataaat taatttagaa cctaaaaaat taattgagca aattggaaaa
     8941 gagaaggata aaaaagatat ttggatagtt ggtggaggcg atatagtaaa attatttatg
     9001 aaagataact taattgatga ttattatata tatatattgc caataatatt ggggagaggg
     9061 atttcactat ttaagtctgg tatagataag actaatttga attttaagaa agctagtaat
     9121 attggagaac ttgtaaaatt ggaatacagt aaaaaacaaa aataaattac atgccaattt
     9181 gttacaatgt tatgaaatga agcatttaaa agacttttgg ctatatgaat tgacattact
     9241 tgtcaaatat gttaacatat aagcgacttg tcggagggtg tattaaaaag aatatgctgg
     9301 agaagagagt gcgttaactc ttttctcctt tttttataaa attaagcatt tataaattta
     9361 gtattattac atgattacac tatctcaaaa attaagaaag gagaaaaata tgaaattaat
     9421 tttaagttat ttaaagaatt ataagttatt aatagtatta aacatactgg ctatatttag
     9481 ttttgcatta gtcgagttgg ggattccaac tataatagct aaaataattg acaatggtat
     9541 tgcaaatcag aatatagctt acataaaaca aatgggaata gtgattgtta ttatttctat
     9601 tataggggtt gtaggtagta tattgttggg ttattgttct gctaaggtat caacaagtgt
     9661 aaccagagat ataagaaatg acatatttga gaaatcacaa gaattttcac atactgaata
     9721 caataaattt ggaatttctt caatgattac aagaacaacg aatgatgcat ttcaaattca
     9781 acaatttgtt aatatactac taaggactgc cttattaact ccagttatgt tcattataag
     9841 tataataatg acaattagaa caagtgtgga actttctata gtattggcta tatcagtacc
     9901 ttttataatt attggagtag caataatagc aaaagtatct caggcaatat cttcaaaaca
     9961 acaaaaagga ctagataaat taaaccttat atcgagagaa aatttaactg gaataagagt
    10021 aattagagct tttggaaatg atgattatga aacagagcga tttgaaaaaa caaatacata
    10081 ttatgctaat gtttctaaga aactatttaa gcttatgtca attactcaac cagcattctt
    10141 tttactatta aatatagctg ttttggctgt attttggatt tcaagtgaga agataaatat
    10201 tggagagctt caagttggac agttggttgc atttttggaa tatcttttcc atgccatgtt
    10261 ttcaattatg cttttttcaa tggtatttat tatgtatcca agagcagagg tatctgcaaa
    10321 tagaataaaa gaacttttaa atgaagaacc attgataaaa aatcctgaaa atggaattaa
    10381 ggatactgaa aataagggta tcatagaatt tgataatgta acttttacct atccagatgg
    10441 agaagcttct gtacttaagg atatttcatt tactgcaaaa acaggtgaaa cagttgcttt
    10501 tataggaagt actggtagtg ggaaaagtac tctaattaac ttgataccta gattttatga
    10561 tgtaacagaa ggtagtatta agataaatgg agtagatata agagaatatg atttaaaagc
    10621 tttaagaaaa aagataggat tcatacctca aaaatcactg ctttttactg gtagcatagc
    10681 aaataatatt agatttggga aacataaggc aggagagtca gaattagaat attccgcaaa
    10741 agttgcacaa gcatatgagt ttatatcaaa aaaacctaga aagtttgatg aattgattag
    10801 tgaaggtgga gcaaatgtat caggaggtca aaaacaaaga ctttctattg caagggctat
    10861 tataagaaga cctgaaatat acatttttga tgatagtttt tctgcacttg actttaaaac
    10921 tgatgcaata cttcgtgcta aattgaagaa ggaaacaaaa gatgcaattg tattgattgt
    10981 tgcccagaga attagttcta ttattgatgc tgataagatt atagtactca atgaaggaca
    11041 agttgtagga atgggaactc ataaagaatt attaaaaaac tgtgaaatat actatgaaat
    11101 tgcaacatct cagttaaaaa aggaggaatt agaataatga aaaagtctaa aggttttaag
    11161 aaaacaattg gtagacttct tccatttgta aagaaatata aattttcctt tattatagct
    11221 attatatgta taatttcagc agctactatg aatgcacttg cacctaaaac tgaaggattg
    11281 ataataacac aattgactaa agatgtaatt agtatcgcaa aaggagtacc aggggcttcc
    11341 gtaaattttg attatgtaac aaaaatattg gtgattttag catgtatata ttttgccaat
    11401 gcaattttta cgtatacttc aagttttcta cttaccaatg ctattcaaaa cacaatgaga
    11461 gatttaagaa atgaagtcga aaataaaata agaagattgc ctattagtta ttttgatagt
    11521 aatagttttg gagatgtatt aagtcgtata tcaaatgatg tagatacaat atcaaatgca
    11581 ttgcagcaga gttttatgca aattgtaaac tcaatcttag tcataatttt ggcactttca
    11641 atgatgttta caattaatat atatatggct cttatagctc tttttataat tccaattagt
    11701 tattttgtat ctaaatttgt agtaaaaaaa tctcaaagta gattttcatt gcaacaaaat
    11761 gctcttggaa aattaaatgg gaaagtacaa gaaatgtata ctggatttaa tgaaataaaa
    11821 ctatatggta aagaagaaga ttctataaaa gaatttaaaa aagtaaatca agaactttgt
    11881 gaaaatggat ttaaggcaca atttatatca agtatgatga atcctatggt ttcattagta
    11941 acttactttg gaattgcagc tgtggcagtt gtcggttcta tatatgcagt aagtggagga
    12001 ataacagtag gaaatttaca agcatttgtg cgttatatat ggcagataaa tcagccactt
    12061 tcacaaatga ctcagttatc tacagtaata caatcttctt ttgcagctat agaacgtgta
    12121 tttgaaattt tagacgaaga agaggaaata cctgatgttg aaaatccagt taagatagaa
    12181 aatgtaaaag gaaatgttac atttgagcat gttaattttg gatatggaga aaattcaact
    12241 ttaatagaag atttaaatgc agaagtaaaa agtggtcaga tggtagctat tgttggacct
    12301 actggagcag gtaaaacaac attaataaac ttattgatga ggttttatga tgtaaagaaa
    12361 ggtgctatta aaattgatgg agtagatata agagatatga aacgtaaaga tttgcgttca
    12421 atgtttggta tggtgcttca agatacatgg ttatttaatg gaaccatttt tgaaaatata
    12481 gaatatggta gatttggagc tactaaagaa gaaattatac aagcagcaaa agtagcaaat
    12541 gttcatcact ttataaccac tcttccagat ggatacaata tgtttttaaa tgaagaagca
    12601 tctaatatat ctctagggga aaaacagctt ttaaccatag ctagagcttt tatatcagac
    12661 ccttcaatat tgatacttga tgaagctacg agttctgttg atactaggct ggaacttatg
    12721 cttcaaaaag caatgagaaa tcttatgaat ggtagaacta gttttgttat agcacataga
    12781 ctttctacaa ttcgcaatgc tgacttgatt ttagttatga ataatggaag tattatagag
    12841 caggggaatc atgatgaatt aatggaaaag ggtggatttt atgagaagct ttataatagc
    12901 caatttgcag acaaagaatc agaatagata acataaactt ttgtataaaa ttttgtatca
    12961 aattcaaata ttaatcatat aattaaaaag agtatcagta agttgttttt ttatagattt
    13021 actgatactc tttgatttta agaccaaagg tacttatgat tggtacttat aattcacaac
    13081 tagtcttgtt acatctgata accagaatta cagtactttt cttttttacc tttttgttct
    13141 ctaacatata acaattcgct taacttatca atgtaataac catcatcact tatgcgaatt
    13201 aaactagtca gataatatcc atctttgctt ctaggaatct ttttatttat tgctaaatca
    13261 atagctgtct ttgctgcttc aaaatgcttt aaatgtgtat gaccttcttc aaaactcttt
    13321 ctagtattat aaactacata acctttttta acttgaaaaa ctataaattc tttcttttca
    13381 tatattttat taaacattca atcagccctt ctcaagatag ttttatagca taacattaga
    13441 agcttctaaa aaattttaca aagaacataa atattttata aattataaat ctaaaactat
    13501 aatttataaa gataactttc tgattattta tatgataatt catatagatt aaaagtaaat
    13561 tatctttagg ttaaacaaaa gtaaaataaa agactagtta taatatttat atgaaaaagt
    13621 acaaaatata gtatatgaat atagaaaatt taaattattt atttacagtt tttatttcca
    13681 taagtatatt taccttcaga taccttgtca tcatggaaat atattctttc acaactttta
    13741 tctccccata gaatgttttt tgttatttta tgggacgtat ctcctgtaaa tacttcagct
    13801 acagtagtat ctgtaacatc acagtaaaga gaagcgaatt ttttaaacca aggattttcc
    13861 tcaaatctta aaagccagga agctccatag gggcaatgta actcttcaaa ttcaggaacc
    13921 catcctagaa atggaacatc agttgtgcta ataatattgt ctgcaattaa ttcatctcca
    13981 ttttcatgag ctctctctct catagcaata gctctttctt cagacctttc aaataaaact
    14041 tttcttaata tttcgctagt tttatcttca ccaaactctt caaataacac tttggaaaaa
    14101 taaaaatata aatcaccaaa atgtttgcat gccatacgaa cttcttcaat agcataatct
    14161 ttgtcaatag tttcattagt tacaacatct tcgttcaaaa aaatcactcc ttagaataag
    14221 tattagttac aatataatta tagaatattt tttataaatt aggaaattaa tttgagatag
    14281 atatattttt aataaatgaa agtataatgc taaaataact tattgttaca aaaatcttct
    14341 aaattaatgt atttaggagt ttccatatga atgagagtag ttctgtaaat atcttcaata
    14401 catccatttg caatattatt aaaaatatct gtgtgctctt ttaatgttgt ttcaagcctt
    14461 ccttcatgtg acaatgaatc gagagctaat ttttttattc tgtacatata attgttaaac
    14521 attttgtcaa attcagtatt ttgagcatat gaaactatag ttgtgtgaaa taatgtatcg
    14581 tattctgcaa atgagtttaa atttttatca cctaataaaa tatctttttg tttatctaaa
    14641 agatgtttta atttacttat agtttctact gctctagctt ctttgtattg acttgtaatt
    14701 aaaaaagtac aaaatccttc tattgcagag cgtatttgga atatctctac aatttcattt
    14761 gctgtaattt gatggattct aaatcctttg cttgggatta tatctataaa tccatcttga
    14821 ctaagtcttt gaagagcatc tcttataggt gttctagaga tacctatttc actagcaagt
    14881 tttgtttcag aatatatttc attttctatc atttctccag atagtatcaa ttcttttaag
    14941 tagtcatatg cttgttcttg taatctttta aatgtagcca tacttaataa taccccccta
    15001 aacaatataa aaatacaaaa acttatataa ttttaagcat acaacatagt gaaaataagg
    15061 tcaatctttt gcaatttaaa ttgcagataa gattaaaaaa tataaaaaat tattgaagtg
    15121 tgaagcatga ttaacattaa aggcagatta attaaaacta attgaaatta gttaaaatac
    15181 aaaactgtaa aagtagtata ttatatttga aaacaatagt atgtattaaa acttatatag
    15241 gatgactaaa tataataaat gactttaaaa attactattt attgttaaaa taaatacttt
    15301 tatattttat attgaccggt atactggtat gtagtatact ctaaaacata aaaaacgttt
    15361 tcaaaaatgt gaagatatgg agggttataa aatgaaattg ggatttattg gattaggaat
    15421 aatgggaaag ccaatggcaa aaaatttatt aaaagatgga tgtaatcttt tggtgtatga
    15481 cattaataaa tctgctgtgg atgaacttat atcatgtgga gcaaaatacg caagtgtttt
    15541 agaaatgggt caagaatgtg atattgtatt tacaatcctt ccaaatggaa ctattgtgca
    15601 ggatatattg tttgggatgg atggattggc aaaaacttta aaagaaggaa gtatagtagt
    15661 cgatatgagt tcagttactc ctacagaatc catattgtgt gcaaataaat taaaagatat
    15721 gggcttagaa tttatagact caccagtaag tggaggggag ccaaaagcga ttgatggtac
    15781 acttgctttt atggctggtg gtaaggaaga aatttataag aaagtagaac cattttttaa
    15841 tataatggga agctcatcta ttttaattgg agataatggt agtggaagtg ttacaaaact
    15901 tacaaaccaa gtaattgtta atttgacgat agcagcagtt tctgaggcat ttgtacttgc
    15961 agcaaaagca ggtgcagacc cagaaaaagt atataaggca ataagaggag gcctagctgg
    16021 tagtacaata ttggatgcga agataccaat gattatgaat cgtgatttta aaccaggtgg
    16081 gaaaatatct attaatttga aagatattaa gaatgtaatg caaacagcac acaatttaga
    16141 tgtaccacta cctatgacaa gtcaattatt agaaattatg cagacattaa aagtacatgg
    16201 acatttggaa gatgaccata gtggaatagc acagtatttt gaaaagttgg caggtgttga
    16261 aataaaaaaa cacaataatt aatatttaaa tatatcaata taattaaata catattatta
    16321 ggattagatt atataaatac agcgaggtta agttatcata gtaaaagaaa aataaaattg
    16381 ataaataata aagtagaatg ataaaggggt aaagattatg attacaggat atggactttt
    16441 gatagcattt gttatatcta taggaatttt acttgtatcg attattaaat ttaaagtaaa
    16501 tccgtttttg gcattactga taacatctat aattacaggc tttatggtta gaatgcctat
    16561 aaatgaaata tcaaccacaa tatcgactgg atttggaaat acattaggta gtataggtat
    16621 tgtaattgga cttggaataa tatttggaaa tattctttca gaatcaagag caacagaatc
    16681 tattgcaaag gggttgttag ctaagactgg agaaaaaaac agtgctttag cagtaactac
    16741 agcaggtttt ttaatttcaa ttccagtttt tatggatgca gcttttgtta taatgatgcc
    16801 tataattaag tatgtttcga gagtaactaa gaaatctcta atggtttttg tatgtgcctt
    16861 aggagttggg actatagtag gtcatgcact tgtaatacct acaccaggac ctttagcagt
    16921 tgcagcaaat gtaaatgcaa atgtaggtag ttttatactt tattctataa ttgttgcttt
    16981 tccagcagct cttataggtg gatggattta tggaaaacgt tttgaaaaat atccagctta
    17041 tgctattgat gagaatgata gagaaaaaaa tttagagcag ggaaaagata gcataaaaat
    17101 tgaagatgat gattcttcta aagtaccagg ttttggaatt tctatgtttt ctttattgtt
    17161 cccaatactt cttatactta tatcaaatgt atttagtatg ttcttagaaa aaggttctac
    17221 aatgagtgga gtacttgcat ttataggaga taaaaacata gcaatacttc ttggaatatt
    17281 ggtggcaata ggatttttaa agaaatacat aaataaacct atgggagatg tagttattga
    17341 agctgctgat tcagcagggc taatattatt gataacaggg tcaggtggtg cgtttggcag
    17401 tgttataaat gcaagtggta taggtaactt cttagttgat actatgtctg gtcttagtat
    17461 atcagttgta gtgcttggat tcttattaag tgccttgctt agaatttctc aaggttctgc
    17521 tactgtagcc ttagtaacaa catcatcaat tttaggacct acaattttgg caactggaat
    17581 gtcaccagtt ttagtagggc ttgctatatg tgctggagga gttggtttct cccttccaaa
    17641 tgattctgga ttctgggtac tttcaagatt tagtggatta tcagttaaag atactttaaa
    17701 ctcttggaca attggaggaa ccattgctgg agttgtagca tttataatgg ttttattgct
    17761 tagtgttata aatggaatta taccacttcc aggtctataa ataacaaact attgtttata
    17821 tcatataaat tacatttaga ctatacaaat tacataaagg ggcgttttaa atgagagaag
    17881 taaaaccaat tagcatagat atacttaata cttttaaaca agtagatgag gacagattaa
    17941 ataaacttct tgcagatgag cttaagcatt tagatagaaa aattgttgta ttagatgatg
    18001 acccaacagg tgtacaaaca gttcatgata tttctgttta tactgattgg gataaggata
    18061 gtatggaaca aggttttaat gagaaaaata gtatgttttt tatacttacc aattcaaggg
    18121 gatttactgt tgctcaaact acaaaagcac ataaagaaat ttcaaagaat attgtggatg
    18181 tatcaaagaa agttaataag gattttataa tcataagtcg tagtgattct actatgagag
    18241 gacattatcc tcttgaaact aatttattaa aaagcgaagt agagagatta tcggagaagt
    18301 tatttcaggg tgaaataata atgccatttt ttaaagaagg tggtaggttt actatagata
    18361 atgtacatta tgttaaagaa ggagaaatac ttgtaccagc aggaatgact gaatttgcaa
    18421 aagataaatc ttttggatat aaatcatctg atataggtga gtggtgtgaa gaaaagacaa
    18481 atggaaagta caaatctagt gatatgatat atatttcgtc aaaagaactt agagagctaa
    18541 atattgaagc tataacagaa aaacttaaaa aagctgaagg attcaataaa attattgtaa
    18601 atgcaaccga ttatgtagat gtaaaggtat ttacaattgc gtttatacga gcagtaaata
    18661 gtggtaagga atttatgttt agaagcgctg ctgccataac aaaaatactt ggcggagtga
    18721 gtgacaagga actacttact aaggatgaat tagtatcaaa aggtaatacc aatggaggaa
    18781 ttatacttgt tggttcacat gtgaataaga ctacccaaca gttagaagaa cttaaaaatt
    18841 gtaaatatcc gatagaattt atagaattta atcaacattt agttcttcaa gaaaatggtc
    18901 taaaaaatga ggtaaaaaga gttataaaag ttgtagagga aaaaataagc aggggtaaaa
    18961 ctgttgctgt ttatactaga cgagagcgtt ttgacttgga tacgaatgat aaagataaac
    19021 agcttatgat ttctgtagaa atatcagatg cagttaccag tataattggt atgcttaatg
    19081 taagaccaaa ttttattata gctaagggtg gaattacttc tagtgatgtt ggtactaaag
    19141 cactaagagt aaaaaaagct acagtaatgg gacagataaa acctggtata ccagtatgga
    19201 tgacaggcaa agaaagcaaa tttcctaata tgccatatat aattttccct ggaaatgtgg
    19261 gagagattag cacacttaga gaatcagttg agatattgat ggggtagtat attagtgtat
    19321 tgaagtttaa acgcttaaat ttactgagtt aaaattaaaa taaactaaaa ttataaatta
    19381 agattaaaaa taatagtata ttaattaggg tttagagtat tttacaaaaa atattctaaa
    19441 ccttaatctt atgtattagt atttttatta tttaacttat gtgtttattt tattatttat
    19501 cttttttaat tgatttaata ttttctttga ttttatttaa aatatctgta tgcttattag
    19561 agcttatgtt ggggaatgct ttgattatac gtctatgaag taatttgtgg ttagacagca
    19621 aagtattaag tttatttttt agctcatcaa tacgattttt agcatagcga taattttctt
    19681 tttctttaag tatttcaaac ctatcttcca aagtatccag tctattttca actaaagatt
    19741 caagtttgtt ttctatagtt tcttgtgtaa taagtttgta gtattctttc ttatctttta
    19801 attccttaat tataagattg atttgtttta acttaccatt taattgtaaa attgtatgga
    19861 ctgtgataac taaatctaaa acaaaataaa tttctataga tatagcaaat ataaatagca
    19921 catttttaga gatagagtta ataaagtcaa ccacaatagg atgtactata agcattacaa
    19981 tcaatgataa tacaccaaac ataagtgagt tttccagaca aactcttccg tgaatattaa
    20041 atttatgttt agaataatcc caccaagtca tattaaatat tttttctaaa ataaagctcg
    20101 tacaatactc cattatgcta gttataatta tactaaacaa aaacaacaaa ggaatagtgt
    20161 gtacaaaagg agtcaaggta gcaactattg ctaatgctcc aaatccatat atagggcaaa
    20221 atggaccatt taaaaaacct ctatttacaa attgtttttg taaaactgaa caatatgtag
    20281 tttcacaaca ccatcctata aagctataaa ttacaaaata aagaaattga atataaaatg
    20341 tactcataag cacctcattt ctagttagta taacactata tactcgatat tttattttta
    20401 tgattaattt actaaattca atttacatat tgattatacc aatttataga aatttatcaa
    20461 atttaattac agttagaaca ttataaacat ggttataata atgaaatttt atttaaaata
    20521 aataagggtg tcaagtataa tcaaactaat agttgtagat actctacact atgttagttt
    20581 aattacattt gacaccctta tatttatact atgactattt acattcgaaa ctagagcaac
    20641 atgtttcaat ataaaggttt actctatcag tttcaggagc aaccaatata ttatcaagtg
    20701 tacataagct accatctttg ttgtatttac aattttttac agtacaaact aattcctcac
    20761 aatttctatt attaacgttg ctagataaat tactataaat agattcatct aaaaatgaac
    20821 tacaacaagt atgtctgtca ttgtgagctt tttcaccatt tataaaaatt ttatctgcat
    20881 aacatatatg gctgttgtta tgtgaacagt taattacatt acagtttatt ctttccaaag
    20941 ttattacctc ctaaacgatt atttattata aattatgtcc atataattac aagaatataa
    21001 ccattaagag aaaactacct tttagaaaat atctattctt agagtaattt gaaatataaa
    21061 tatattttct tctaaaattt acctaaattg tataaaaact tacagcttgg agaaaataat
    21121 agaaaaagga ggcgtaatca atgtttgaaa agaagaaaaa agaattattt ccttggaaaa
    21181 tatctgcctt aatactggtt ggagtatttg tattaagtag cggagtttat atagggacaa
    21241 gattaaaaca caatggagtt caaactggtg aagttgtaag tactagtgcg aatatgaatg
    21301 aaaagtcaaa agaagctttt ggattaaata aaaattgtga agtttggctt caaagtgaag
    21361 gcgaaaatga aactaactat aagagaacac ttacaatggc aggaatagtt ccagagtcat
    21421 tattagataa gacgaagtca gaaatagtat catattttaa aaaagaatat ccaactaaaa
    21481 aaataaagag tatggataaa aatgaaatca tattggtaga atctaaatat agtgataata
    21541 aagatgtaca agcaatggat gttactataa aaggtaaata tactattgaa aatgataatg
    21601 gaaatatagc actatataaa tatgatgata gtggaagcaa aacattagta gaaaaaacaa
    21661 aaataagagt agattcactt cctaaaacag ttcaagatga aatcaaaaaa ggtgtagtta
    21721 tggatactga agaagaggcc tattcaagat tagaagactt tgcaagttaa tagtaaatta
    21781 atattatttt aagttaaata ctaagtaagt taaatactaa gtaaattaat aataaataat
    21841 ttaatattaa atagattagt attaaataga ttagtattaa aattgtagtc acatttaatt
    21901 cctataaaaa tacaataatt tatttgtaat aaatataaaa gttatattga ggtaaaatct
    21961 tgctttgata taactttttt atttgatttt atattttaca tagagcattt tatgatttgt
    22021 taatattatt tatgatataa tgatttagaa cataagtttt tatgagaaat acattttggt
    22081 atttctttta tttttagggg gaattttaaa aaatgataat attggggatt gacccaggta
    22141 tagccatagt tggatatggt ataattgaat acaaaaatag caagtttaaa gtaatcgatt
    22201 atggagcagt tacaacacct gcccatatga atatatcgag aagattggaa cttgtgtata
    22261 aaggaattga tacaatagta aagaattaca atatagatga agttggaatg gaagaattat
    22321 tctttaacaa gaatgtaaaa acagctataa cagttgcaca agctagaggt gttactatgc
    22381 ttgcatgtgc tcataatggg aagcctgtat atgaatacac tccacttcaa gtaaaacaag
    22441 gtgtagttgg atatgggaga gcagataagg cacaagttca acagatggta acttcatttt
    22501 taagtctaaa aaaagttcca aaaccagatg atgttgcaga tgctttagct gtggctattt
    22561 gccatgctca ttcaaacaaa cttgaaaaaa ctctaaagaa tataggtggt aagtatgtat
    22621 agttatataa aaggtacagt agaagaaatt tatatagaca gtatagttgt tgaaaataat
    22681 gggataggat ataaaataaa tgtatcatcc aatacaataa tgaatttaca agtgggagaa
    22741 gccacaaaaa tatatactaa attaattgta agagaagatg atatgagtct ttgtggtttt
    22801 gtaagtaggg aagaacttaa gatgtttgaa ttactaacat ctgtatctaa aataggacct
    22861 aaagtggcgc tttcaatact atcatttgca tcaccagcac agttaggagc atatatttta
    22921 agtgaagaca ttggaaaatt atccaaagct cctggtgttg gtaaaaagac agcagaaaga
    22981 atcgtcttag agcttaagga taaggtagac aaaaataaca tagagtttga accaacatta
    23041 ttatcacaaa aacctacctt gatatcacaa gatgagtctg tagatgcact tgtagcttta
    23101 ggctatactt tgtcggaatc aaaagaggca gttcaaaaat gtaaaaaaga tggtatgaat
    23161 acagaagcta ttataaagaa agcacttaca tatataatga gtaaatcctt gaaatagtat
    23221 ttggatttta taatatttgg attttaaaac aatatttaag gagtttatgt aaatttgaaa
    23281 atatataaag tagattttca aaagtatgag aggaggggta taaatgcaag gttttgaaga
    23341 tgaaaatagg ataataactt ctacaatgaa gatggaagat atagatatag aaaatagttt
    23401 aagaccaaaa actttagaag attatttggg tcaagaaaaa tccaaagaac aacttagtat
    23461 atttatagaa gcagctaaat ctagaaatga gcagttagac catgtgcttt tatatggacc
    23521 accaggtctt ggaaaaacta cactagcaag tataatagca aatgagatgg gggtaaattt
    23581 aagaattacg tcaggacctg caatagaaag agctggagat ttggcagcca tacttacgaa
    23641 tttaaatgaa aatgatgttt tatttataga tgagattcat agaatcaata gaagtgttga
    23701 agaagtttta tatccagcaa tggaggattt ttgcctagat ataataatag ggaaagggcc
    23761 atctgctaga agcataagac ttgaccttcc taaatttaca cttattggag ctactacaag
    23821 agctggtatg cttacaaatc cacttagaga tagatttggt gttatttgta aattggacta
    23881 ttatacagtt gatgaacttt caaaaatagt acttaggtct tcaagcatct tagatgcaga
    23941 gatacagtct aatggagcat tagaacttgc aaaacgttca agagggactc caaggatagc
    24001 aaatagatta ctaaaaagag ttagagattt tgcacaagtc agagctgatg gaaaaataac
    24061 tgataaggtg gcaaaagatg ctctggaact tttaggagtc gatagtttgg gtctcgattt
    24121 tgtagatgag aagcttctta tgactataat agaaaaattt agaggaggac cagtgggttt
    24181 agatacattg gcagcttcta tcggagaaga tagaaataca atagaagatg tatatgagcc
    24241 atatttgctt caattaggat ttataaatag aggtcctaga ggaagagtag ctatgccatt
    24301 agcatatgaa cacttaaaaa ttccatatcc taatgaaaaa taaaaataga ttatacgagt
    24361 atttataaaa taaaagagtc attaaattaa aatatttcta aaatggctaa aaattcaaac
    24421 atatccacaa aatggtaatt atatctgtag atactgtgga taaacacgat aaaaattgtg
    24481 tgtattgtat ctatatattg tggataagac atgtatatcg tggaaaaata tctatatatt
    24541 gtggatatgg tgtctgttaa aaatagcaat ttcttgatag attatggtaa tattgtggaa
    24601 aataagttgt ttttttgata ttattgtagc tggagaaatt ttaagagagg aagtttaaca
    24661 ttgaaaacaa gtgattttaa atttgactta ccacaggaac ttatagctca agttcctata
    24721 gaagatagag ctagctcaag attgatggta ttagataaag agacaggtaa tattgaacat
    24781 aaagtattta gagatataat agaatattta aatccaggag attgcttagt attaaataat
    24841 actagagtta taccagctag attaatagga gagaagcttg aaactggagg taaaatagag
    24901 tttttattat taaaaagaac agaggaagat acttggcagg ctttagtaaa accaggaaaa
    24961 agagcaaaag tagggactaa attttctttt ggaaatggaa agctaattgg agaagttgta
    25021 gatttatcag atgaaggttc aagaattata aaattccatt atgatggaat ttttgaagaa
    25081 atattggatg aacttggaaa tatgccacta cctccatata tcactgctag actagatgaa
    25141 aaagaaagat accagactgt ttattcaaag cataatggtt cagcagcagc acctacagca
    25201 ggattacatt ttacagaaga attgttaaat aaaataaaag aaaaaggtgt agatatagcc
    25261 tttgtaactc tacatgttgg cttaggaact tttagaccag taaaagtaga ggatgtttta
    25321 aatcacaaga tgcattcaga atattatatg gttagccaag aagcagctga caaaataaat
    25381 agagcaaagg aaaatggaaa aaatgtaata tgtgttggta ctaccagttg tagaactata
    25441 gaatcagctt gtaatgagta tggaaagatg aaagaaacta gtggatggac agaaatattt
    25501 atatatccag gttataaatt taaagtttta gataaactta ttactaattt tcacttacca
    25561 gagtctacac taataatgtt agtaagtgct atttgtggaa aggataatgt tttaaatgca
    25621 tataatgagg cagtaaaaga aagatataga ttcttcagtt ttggagatgc tatgattata
    25681 aagtagggag gtaatggatg catagtttac aaaaacaaaa aaagaataac cctatattaa
    25741 agattgcttt tatatttgaa cgtgttttag cagtagtagt tctaatagca gtatttttag
    25801 gaactataga tgttctaaga cttatgtggg gagcatatat agttgatttt caaaatccag
    25861 tacaatacag tcagcttaat gattttttag cacaaatatt acttttagta ataggtgttg
    25921 aacttgtagt aatgctatct cttcatatac caggagcatt tattgaggca ttactttacg
    25981 caattgctag aaaaatgttg ctactgccta aaaatgaagg tatgatagat gttttaatag
    26041 gagttatagc tatagcagga ctatttgcta taaaaaagtt tttagtaaca aaagacaaga
    26101 gtgctcttaa tataatgagt attcatgatg aagaagcaat taaggaacaa cagaacaaag
    26161 aagacgaaga taaatctatt tagacaataa attatatgaa tagcttatta caaatatatc
    26221 ttaagaaata atgagaaata aatatgatta aggagaactt tatgtacgca gtaagatacg
    26281 aattgataaa gacttgtaag cagagtggtg caaggttagg aagattacac actccacatg
    26341 ggattattga aacaccaata ttcatgccag ttggaactca agcaactgta aaatctatga
    26401 caccagaaga attaaaagag attggttcac aaataatact tagtaacaca taccatttat
    26461 atatgagacc aggtcatgaa cttataaaaa gagctggtgg tcttcataag tttatgaatt
    26521 gggataaacc aatacttaca gatagtggag ggttccaggt atttagtcta gggcctttaa
    26581 gaaagataaa agaagaaggt gtagaattta ggtcacactt agatggttca aaacattttc
    26641 taactccaga aaaagctatg gaaatacaaa atgcattggg ttcagacata atgatggcat
    26701 ttgatgagtg tgcaccatac ccatcagata gagaatatgt aaaaaattct ttagaaagaa
    26761 caacaagatg gttaaaaaga tgtaaagatg cccataataa taccgacaag caagctttat
    26821 ttggtataat acaaggtggg atgtataaag acttaaggga acaatcagcg aaagaaataa
    26881 ctaatattga tttaccagga tatgctattg gtggacttag tgttggagaa ccaaaaccac
    26941 ttatgtatga cgtgttagag catacaactc cacttatgcc aaaggataaa ccaagatacc
    27001 taatgggagt tggtagtcca gatgacttag tagaaggtgt tataagagga gtagacatgt
    27061 ttgattgtgt tctacctact cgtatcgcta gaaatggaac tgcaatgact agccaaggta
    27121 aagtagtagt tagaaatgca acttatgctg aagactttac tccacttgac cctgaatgtg
    27181 attgttatgc ttgtaaaaac tattcaagag cttatataag acaccttata aaagcaaatg
    27241 aaatactggg tgcaagatta ataactactc ataatcttca ctttttatta aatttaatga
    27301 aacaaataag acaagcgata atggaagata gattacttga ttttagaaat gaattttttg
    27361 caaaatatgg atatgaaata taggttttgc actattaatt ataatcattt taatatataa
    27421 tattcaatag tactcaaaga tagagaaagt aaattgttat ataaacattt attatgattt
    27481 acaaaagagt ataatttaca aaggagtgaa aaattaatgc cacagcaaca aataattatg
    27541 agtataggtc tatgggtagt agtaatcgca atcttttatt tcctaatgat tagacctcaa
    27601 aagaaaaaag acaaacaatt aaaagaaatg agaagcagcc taagtgtagg agacaaggtc
    27661 attacaatag gtggaatagt agctaatgta gctaaagttg aagatgatgt tgtaatatta
    27721 gaattaggac caaatagaac taaagttcca tttgaaaaat gggctatagg aactgttaaa
    27781 tctaaaaaag aagaaataga agaagactaa attagtaatt taaaagcctt ttctcgaata
    27841 tatattaatt tgagaggagg tttttttatg ggaaaatcta attatatttt taaaggtcta
    27901 ggatatgcat atataataac tttggctgtt ttattagtgt ataacttatt tttgacattt
    27961 acagatattg gtggagataa tataactatg gtctcatcat ttataactac tatttctgca
    28021 gctataggag ggttttacac ttcaaaacac atgaaagaaa aaggacttat gtatgggctt
    28081 ttagtaggac ttttgtacat agtatgtatt tttttgactg tgtttttagc acaagaaaag
    28141 tttgtatttg aaatagggat gatttataag ttattgctaa tatcagcagc aggaggcatt
    28201 ggaggcgtat tgggtgtcaa ctttaaatag agtttatatt aaagtagttt tatgatataa
    28261 tcaataggaa acaattagaa atggaggcgt tgaaaatgga aaataaacat gtaaaaactt
    28321 tatctcaagc tactttaaag caaagtgctg caaaaggtgg atgtggagag tgtcaaactt
    28381 cttgtcaatc agcttgtaaa acttcttgta cagttgcaaa tcaagagtgt gaaagataga
    28441 aaattattga tttagttgtt tgtaagaggg tatctcaaag tgattctttt atcattatga
    28501 ggtatcctct tttattttca attattatat aaaaatatat attctattta tgtagaagta
    28561 aatttgattt cttatcgttt aattatgcaa aaatatctaa tcatgtaaga gcatttattt
    28621 taaagtagct actcttatat tttattttt
//
LOCUS       DAMJWS010000051        28098 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMJWS010000051 DAMJWS010000000
VERSION     DAMJWS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28098
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.051"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(110..625)
                     /locus_tag="PC399_002279"
     CDS             complement(110..625)
                     /locus_tag="PC399_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF2593136.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(988..2058)
                     /gene="leuB"
                     /locus_tag="PC399_002280"
     CDS             complement(988..2058)
                     /gene="leuB"
                     /locus_tag="PC399_002280"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF2593137.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGSVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(2065..2556)
                     /gene="leuD"
                     /locus_tag="PC399_002281"
     CDS             complement(2065..2556)
                     /gene="leuD"
                     /locus_tag="PC399_002281"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF2593138.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2571..3848)
                     /gene="leuC"
                     /locus_tag="PC399_002282"
     CDS             complement(2571..3848)
                     /gene="leuC"
                     /locus_tag="PC399_002282"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF2593139.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3805..5547)
                     /gene="leuA"
                     /locus_tag="PC399_002283"
     CDS             complement(3805..5547)
                     /gene="leuA"
                     /locus_tag="PC399_002283"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF2593140.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5671..5917)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6065..6511)
                     /locus_tag="PC399_002284"
     CDS             complement(6065..6511)
                     /locus_tag="PC399_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593141.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTNESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6532..7251)
                     /locus_tag="PC399_002285"
     CDS             complement(6532..7251)
                     /locus_tag="PC399_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2593142.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7369..8643)
                     /locus_tag="PC399_002286"
     CDS             complement(7369..8643)
                     /locus_tag="PC399_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF2593143.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(8843..10210)
                     /locus_tag="PC399_002287"
     CDS             complement(8843..10210)
                     /locus_tag="PC399_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2593144.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(10460..10924)
                     /locus_tag="PC399_002288"
     CDS             complement(10460..10924)
                     /locus_tag="PC399_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2593145.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(10929..11558)
                     /locus_tag="PC399_002289"
     CDS             complement(10929..11558)
                     /locus_tag="PC399_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2593146.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRIIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(11592..12479)
                     /locus_tag="PC399_002290"
     CDS             complement(11592..12479)
                     /locus_tag="PC399_002290"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2593147.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(12508..13746)
                     /locus_tag="PC399_002291"
     CDS             complement(12508..13746)
                     /locus_tag="PC399_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593148.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(14130..15272)
                     /locus_tag="PC399_002292"
     CDS             complement(14130..15272)
                     /locus_tag="PC399_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2593149.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(15413..16279)
                     /locus_tag="PC399_002293"
     CDS             complement(15413..16279)
                     /locus_tag="PC399_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2593150.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(16613..18160)
                     /locus_tag="PC399_002294"
     CDS             complement(16613..18160)
                     /locus_tag="PC399_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2593151.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(18153..19289)
                     /locus_tag="PC399_002295"
     CDS             complement(18153..19289)
                     /locus_tag="PC399_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593152.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(19552..20649)
                     /gene="dinB"
                     /locus_tag="PC399_002296"
     CDS             complement(19552..20649)
                     /gene="dinB"
                     /locus_tag="PC399_002296"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF2593153.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVDVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(21039..22544)
                     /locus_tag="PC399_002297"
     CDS             complement(21039..22544)
                     /locus_tag="PC399_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF2593154.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(22806..23156)
                     /locus_tag="PC399_002298"
     CDS             complement(22806..23156)
                     /locus_tag="PC399_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593155.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(23379..23570)
                     /locus_tag="PC399_002299"
     CDS             complement(23379..23570)
                     /locus_tag="PC399_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593156.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(23688..24602)
                     /locus_tag="PC399_002300"
     CDS             complement(23688..24602)
                     /locus_tag="PC399_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2593157.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            complement(24736..26232)
                     /locus_tag="PC399_002301"
     CDS             complement(24736..26232)
                     /locus_tag="PC399_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2593158.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            complement(26512..28002)
                     /locus_tag="PC399_002302"
     CDS             complement(26512..28002)
                     /locus_tag="PC399_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2593159.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
BASE COUNT         9170 a         5138 c         2882 g        10908 t
ORIGIN      
        1 tctttttagt ataactaatt tatttttatc ttgtattttt attgtatttt tgaattttat
       61 tttttctaaa tataaaattt atgttttaaa atgtaaaatt tttatttaat caaagttcaa
      121 tatattccac tttttctttc gattcttcaa aaaaccattc ttcattagaa aaatcacctt
      181 ctgcaatttg tccaataggc attttcatat actttccagt tgctgtcact gctatatctc
      241 catttggtag aattatttct cctgtacctt caaataattt tctgttttct ttagttattc
      301 ttccaacaac ttttatagta gcatctgttg gtactggttt cttatatttc aactctaaag
      361 atactgttac tccccatatt tggtcatcat ttatacttac agctcttcca atagtctcat
      421 ccaatattgc agcagacata cctccatgaa ctctcccagg ataactttga tgccaatcct
      481 ttgtattaca aattgataca agctctccat tttcaagttc ataaaaatct gctttaagtc
      541 ctagctcatt ttgtgttcca caaactaaac aagatttact actattttgc ttcttcaaaa
      601 ctttatactt tgccaaaaaa tccatctcct aaccgacatt agtagtcgct taatattaaa
      661 taattctaaa atatattata taatattaat aaaatccctg caaacttcta ataataaaaa
      721 taggctactt caatatgata agtttaattt aaagtattat tttccaaaaa ataactttat
      781 caaaatagaa actcaaagta gatataaacc tctttttcgt tattaaaata gtaaaaatat
      841 atttgactta atataaaaaa gagtgtctca ttaactaata aagtttattt tgagacaccc
      901 ctcataaaac aaattatact acaatagatt tttactaatt ctacaagtta tatatttaaa
      961 ttatattatt caaattaaac tataattcta aatttcattt actattaatt cgcccatttc
     1021 ccttgttcct acaacatttc ccacaccatt atagatatca atagttctat aacctttatt
     1081 gagtacactt tgtacagcat cttcaataca cttagcttct tcttctaagt taaaactatg
     1141 tcttaacatc atgcttacag atagaatagt cgcaattgga tttacaatat cttttccagc
     1201 tatatcaggt gcacttccat gtattggttc atacatacca aaactatctt ctctaagtga
     1261 tgctgatggt aacataccta tagaacccgt aatcatagat gcttcatcag atattatatc
     1321 tccaaatata ttattagtca ctattacatc aaattggcta gggtctttta caacttgcat
     1381 agcagcatta tcaacataca tatgtgacag ttctacatct tcaaactctt tagctaagtc
     1441 ttccattata cttctccaaa gtcttgaact ctcaagcaca tttgccttat ctacacttat
     1501 aagtttttta tttctatttc tggctatttc aaaagctctc ttaccaattc ttcttatttc
     1561 attttcgtta tatatttcta catcataagc attttcgata ccatcaatga tttttcttcc
     1621 acgttctcca aaataaatgc cccctgttaa ttcccttaca acaacaaaat caatgccttt
     1681 ctcaactata tccgttctaa gtggtgaagc ttcttttata ctttcataca ttgtagctgg
     1741 tcttaaattt gcaaataaac ccaatgactc tcttaatttt aaaagaccag actctggtct
     1801 tttatctccc ttacatttat cccactttgg tcctccaaca gaaccaagca atacagcttc
     1861 gttttttcga caagtttcaa cagttttatt aggtagaggt tctccagttt catcaatcgc
     1921 gcatcctcca gctaatacat attcacaatc gaatctatga ttaaatttac aacaaatttt
     1981 atttagaacc tttatacctt catctataat ttccggtcca acaccatcac cttttatgac
     2041 tgctatatta caattcatac tccactaccc cctatttgct tttattgagt taattaagcc
     2101 ttcatttaat ataatatttt gcataaactc aggaaatggt tctgctttat attcttttcc
     2161 ttttgttata ttttttatca cacctgtaga aaaatctact tctatattat ccccttcatc
     2221 tatgttattg gcagcttctt cacattcaag tataggaagc cctatattta tagagtttct
     2281 aaaaaatatt ctagcaaaag tagaagcaat cacacaggat actccacttt cttttattac
     2341 aattggtgca tgttctcttg atgaaccaca accaaagttt ttagttgcaa ctataatatc
     2401 tccttttttt accttagaaa taaatttatc atcaatatcc tccatacaat gcgttgctag
     2461 ttctttataa tcagctatat ttaaatatct agctggtatt attacatctg tatctatatt
     2521 gtcaccgaat ttaaaaacac ttccattggc aatcataaat tttacctccc ttaaattatt
     2581 tctgatggtt ttgatatttt tccagttata gctgatgctg ctgctacagc tgggcttgca
     2641 agatacacct ctgaatcaac atgccccatt cttcctacga aattcctatt tgttgttgaa
     2701 atggattttt ctcctgctgc aagtataccc atatgtcccc ctagacatgg accacaagtt
     2761 ggtgtagaaa ctgctgctcc tgcctctata aaagtggtaa tatacccttt ttctatagct
     2821 tcaagatata ctttttgagt tccaggaaat atcataactc taactccatc tgctactttt
     2881 ttgcctttca ttatctcagc tactacttca aggtctgata ttcttccatt tgtacaagag
     2941 cctattacaa cttggtctat ctttatatcc ttatctatct catctactgt tctagtattt
     3001 tcaggaagat gaggaaaggc tactgtttct tttagttcag ctaaatctat ttcgtatact
     3061 tcatcataga ctgcatcttc atctgcttca tatatattta catcttttgt catagttgaa
     3121 cacttatgtg aatttatata ttccatagtc ttatcgtcta ctggaaaaat tccatttttt
     3181 gctcctgctt ctattgccat attacaaata gtaaatctat catccataga taagtattct
     3241 actccatctc cacaaaattc cattgacttg taaagtgctc catcaacacc tatctcccct
     3301 attatatgaa gtattatatc tttaccactt atccatttat ttggtttatt cttcaaaaca
     3361 aattttattg ctccaggaac cttaaaccaa cattttcctg tagccatacc tgctcccata
     3421 tcagttgaac ctacaccagt agaaaatgct cctaatgctc catatgtgca agtatgagag
     3481 tcagcaccta taattacatc tccagcagtt actatacctt tttcaggaag aagaacatgc
     3541 tctattccca tatctcctac atcaaaaaaa tttacaactc cttttgattt tgcgaaactt
     3601 cttgtaaatt tacattgttc tgcactcttt atatctttat taggtgtaaa gtggtctaaa
     3661 accatcacta ctttagtctt atcataaaca tcttcaattc ctaatttttc aaattctcta
     3721 atggctactg gtgatgttat atcattacct aaaactacat ctaaatctgc ttcgattaat
     3781 tgccctttct taacttcatt taatttagca tgtttggcta atattttctg cgtcatagtc
     3841 atccccattt tttatttcct cctcaatata acttttattt attgcattca taagtgcatt
     3901 aatagctgct gtatttatgt tttccgatat acttacacca aaatagactt cattattgtg
     3961 ctctattcct acataagcta cccctcttga atgtgaccct tcctctaatg catgttccca
     4021 gtaatatcta aatttataat ctttcatgcc acattgcttt agtgcattat taaatgaatc
     4081 tacaggtcca tttcctatac caacaatcct ttgttcattt cccatataac ttattcttgc
     4141 agtcatctca atcatattag tatcattaga atcatcattt tcatagttta attcatccat
     4201 tgattttatt ttatacttct taactttata agggctctca acttcaatat attctttctt
     4261 aaacaaatta aatattgctt cactactaag ttcttcccca attctatcag attccatttt
     4321 gactacattt ccaaaatcag aatgcatatt tttaggtatc ataaatccat aatctgtttc
     4381 cattatataa acagctcctc cttttccaga ttggctattt atccttataa tttctttgta
     4441 ttctcttcca atatcatgag ggtctattgg taaatatgga acttcccagt aatcattatc
     4501 tctgtttttc attgctatca tgccttttct aatagcatct tgatgtgaac ctgaaaaagc
     4561 acaatgtaca agattacctg catatggatg tctatcatgt accataagtt ttgtacagtc
     4621 ttcatatata cctattatct tatctatatt gctaaaatct agctctgggt ctattccttg
     4681 tgtataaaga ttaagtccca catttactat atccatattc ccagttcttt caccatttcc
     4741 aaatagtgtt ccttcaagtc tatcggcacc agccaataat ccaagttcac ttgcagccgt
     4801 acaagttcct ctatcattgt gagtatgcaa acttagtata atagaatccc tacaacttat
     4861 atttttacaa aaccattcta tttggtctgc atatacgttt ggtgttgcca tttcaactgt
     4921 tgatggaaga tttattatta cttttttttg aggagttggt ttccaaacat ctatcactgc
     4981 ctcacatatt tcaagtgcat aatccatctc tgttcctgta aaactctctg gtgaatattc
     5041 aaaagtaaat tctgtttctg gatatttttt tacttcttca ttaaataatt ttgctccttc
     5101 aactgctata tctattattt cttgtttact catattaaaa accacatctc tttgaagaac
     5161 agatgtagag ttatataaat gtataattgc ttttttacat ccccttattg attcaaatgt
     5221 tttttctata agatgagccc tagattgtgt aagtacttgt atagttacat catctggaat
     5281 catattctca tctattaatt ttcttaaaaa tgtatactct gtatttgatg ctgatggaaa
     5341 tcctacctct atttctttaa atcctgtatc aataagcatt ttgaacattc tgactttttc
     5401 atttacactc ataggtgttg gcagtgactg atttccgtct cttaaatcga cactacacca
     5461 aattggagcc ttaaatattt cattatctgg ccatttacga tttactattt gcattttatc
     5521 atatttttta tactttccac acttcatgtt atttccccct ttttctgttt tttgtattca
     5581 gtatacctca tatcgacgca atcctgcaca attcataagg ataaatgtca attttatcat
     5641 tagaataaaa aaaacctcat ctctaataaa attagagacg aggttttgct cgcggtacca
     5701 ctctatttag ctttctaaaa agctccctta gtaatagcca atacaataat tataatgact
     5761 atatttccta taacgtggaa ttactccgat tattctaaga tttaaatctt agaaaacagc
     5821 tcctagacaa gttcagaact atcaactact aaatcacacc atcctttagc tctctgaaag
     5881 tttcattgtt cttactactt ctattcatca cttttttaat catttaaatt ttctaaatat
     5941 tttgatttaa tagttattat atacacatta aaatgtattg tcaacacaaa aaataattat
     6001 ctcaattttt tatttttgat aagcaattct ataaaacatg ctaactattg aaaacagtat
     6061 gatattatgc cttaaacttt ttcatctttt catctatgat attcaataat tttttttcat
     6121 cttccaaact caatgccctt aatctaattt tataattaga ttcgttagtg tatatattta
     6181 atatattttt tactacttct attttttcta ttatcttata attaaacttt tgactttgtt
     6241 tagattttat actgaatata cattgattat ttattaatat tgaatgatta tcgagagtaa
     6301 tatatacttt tccctcatat ttatttgaaa aatcacgacc ttgtttaaat agaaaaactg
     6361 ctgttattat acataaaata tataatattg tcgttgaata tataaaaatt gtagctaaaa
     6421 ttataagtgc taaaaccata tatatagtca ttcctttttt aaaataacca ttgtcataat
     6481 aattcatata gataaactta tttttactca aattttttca ctcccaagat tctaaaaatt
     6541 tatatcaaaa aattctataa tttcatcttc atgataattt atgactaatt catctggaaa
     6601 atctactgct tctaaaactt tttctgcatt ttcaaattct cctatagaat aacatatatg
     6661 agagtcagta ccaagaatta ctcttgcacc atatgtttta cacaatgtca acatatgact
     6721 tacattttcc caagtacccg ttctatgaga gtttgaactt aatgatgaat tatttatttc
     6781 taaaaggata tttttatctt tagctttttt tactataggt tcataatcta aaggatacct
     6841 actgtcatct ggatgtccaa ttatttttac cttaggttta tccataactt ttaaaatagc
     6901 atttgtattt tcttcttttg taccagaagc tatacaaggt ctatgcaaac ttgcgattat
     6961 ataatccaaa tgttgcaaca tatcttcttg aacatctaaa ttaccattat aatccattat
     7021 atttccttct attcctctaa gaatctttac gccttgcact tcacgtggaa gcaaatgcaa
     7081 attataaaaa tagaatggat gtggtcctcc tggcatatta ggtccatggt ctgatattcc
     7141 taagtatttt atattcttct tactcgcctc ttcgacattt tccttgaaag tactatgtgc
     7201 atgtccactt attagagtgt gacaatgtac atccataata gactttttca ttaaatttcc
     7261 tcctaatatt ttacataaat tcgtgaaaat agctaaaggg ctatttcaaa actaaagctg
     7321 tcataataga cagtgtaacg ttttaaaaca gccccatttt caattatttt acacttctaa
     7381 atctttaaat ttatctttaa ataattctcc aatacttcct aaagattcct ctacttctag
     7441 gtattctgtt atatcctctt ctggctctct agttgcttct tttattgaaa ccaacattct
     7501 cttagtcttt ggtttaaaat caagaatctt aactttaacc tcatctccaa tattaacaac
     7561 attagtaact ttagccactc tagtatcact caattcagat attggtaaat acgcatctac
     7621 accttcttta atttgaacaa aagccccttt ttctattatt ctaagaactt tagcattgac
     7681 aacatcatca atttgtactt cttttgctat taaatcccaa gggtcattat ttatgtcttt
     7741 taatgccaag gcaatttttt tagtctcttt atttacatcc aacacataaa cttctacttc
     7801 ttgtccctct gaaacaacat cttcaacact ttcaactctt gtccaagcta aattatttat
     7861 atgtgctaat ccttcaatcc caccaatatc cacaaatgca ccataaggca ttatctttgt
     7921 caccttacca tctctttttt gtccaacttc aagagaatta aataagtctt ctttagctgc
     7981 tttgatacga gctttttctt cttctctttc ttgcttaatt cttgctcttt cagcttcttt
     8041 ttctgcctta atttgagctc ttctttcttc tctctcttta tttatacgct cttgtaatac
     8101 atctctatga gatgctacaa gtctattttt tctaacatcc aattctttta tataaacttc
     8161 taagttaaga ccaacaaatt tagatgtatc tgtaataaat tttgtatcaa gttgagaaat
     8221 tggtataaaa aatgtatatg tattgtaatt agcaaataca cctttatcta tattcttttc
     8281 aacatttaca gttattattc tatgttcatc ataagccttt tgtaactcgg caaaatcatt
     8341 tcttttgtca agttgtaatt tagataaagt tattgtacca tctttttgag atacttttgt
     8401 tattacagca gttatttcat cacctatgtg gtatatatct tctatatatt gatttttttc
     8461 tatgttcaat tcacttatag gtattattcc atcaaaccca tagtctaatc ctaattgtac
     8521 ttcatcattt ctcactgcag ttatttttcc agttgtcaat tctccaactt tcactttgct
     8581 gaatacttgt tcttgctggt caagtaattc ttgcattgtt aaatcatttt ccattataaa
     8641 catcctttcc tcttattgat tatcatatct gtaaatatta taccattaat attgcaaata
     8701 cttacatttt tcttactata atttttaaaa taaattcaac ttcatatatt tatacattat
     8761 agaaaatatc ttattaaatt aaaatacaaa ttctaatttt tatctaataa aatattttca
     8821 taaaaaattt atattgtata tcttattctt ttattgcttt aggttttgat agaaaacatt
     8881 tataaaatat aaagaaagaa attgctgtta tagtagcagc tgttatatct gcaactggct
     8941 ctgctaaaat tactgcaaaa agtttttcat taaagaatgt tggaagtata aatattaaag
     9001 gtattaaaag tacaatcttt ctaagtagag ctaaaaccaa agatattgtt gcttttccaa
     9061 gtgctaaaaa tgtctgttga caagctattt gaatcccaaa cataaaagca ccagcaaaat
     9121 atatttttat actccaacta gtcatactaa gtaactgcgg attcttatta aatatactaa
     9181 caaaaaatgc aggaaatatc attaataaaa gccataaaat agtagaatat gtaaaacatg
     9241 acaacaaaca caacttaaat gtctttttta ctctatcaag ttgacctgaa ccataattat
     9301 aacttattat tggttgtgct ccttgtgtaa cccccatatt aggaagcata agaatttgca
     9361 ttatactgct cattatagcc attgaaccta ctgctaaatc tcctccatac atttgtaatt
     9421 tactatttaa ggctataagt actaaacttt ctgtagattg cattataaat ggagagattc
     9481 ccaaaccaag cactggtaag atgatacttg cttttggtat catatacttc ttttttattt
     9541 taagtatact tttctttcca aagagaaaca taagtaccca aatagcagaa acgccttgtg
     9601 caattatagt agctaatgca gctcctttta cacccatatt aaagccaaat ataaaaatcg
     9661 ggtcaagtat tatgtttatt aaagcaccta tagcaactgt catcattcca gttgtagcaa
     9721 acccttgggt atttataaaa gtattcatac ccattgaaat ctgaacaaac actgtaccta
     9781 ttaaataaat atttaaatag tccattgaat atccaattgt agattcactt gctccaaaca
     9841 tccaaagtaa aggctctttt ccaatcttaa aacctatagt caaaagtatt gctaaaatca
     9901 ccagtacaga aaaactgtta ctcattattt tctctgcctc atcattgttt tgctcaccca
     9961 tttttatagc agctaatgga cctcctccca taccgattaa ataactacat gcagttatta
    10021 taattattat tggaaatgcc actccaacac cagccatagc aacttctcca ttttccattc
    10081 taccaataaa aatcctatcg actatgttgt ataaaacatt tacaatttgt gcgataatag
    10141 caggtgtagc aagtttaaat aataatttac ccacactttc actacctaag ttagtgtttg
    10201 aattttccat tttaaaacct cctcaatcca ataattaaaa tgtatcacta aaattttatt
    10261 ctatataaaa atttcatttt attagcagac aaatttagag tactttccct acaagtaata
    10321 ctatactata tttcttttgc catagtaaac attttagaac taactgaagt taaatacttt
    10381 atctcatttt ttatttatta ttaaaaattt ttcacacata ctcataaaaa aactatccaa
    10441 gtatattact tagatagttt tatattatat gtaaagaaag agctgaaact ataacccctg
    10501 ataacaaatt gccaagaaat actcccataa aagcatcttt tattctcatt tttaatatgg
    10561 aagctatagc agatgcagtc cacgttcctg tagttggcag tggtatacct acaaatagta
    10621 ttataccaat tatcgaaaca ctttttaatt tcttcattct tgagtttatt tttctatcaa
    10681 ttacatcagc aatcccttta aatagtttct tccctcttaa aaattgtaat acatgtctga
    10741 atgtcaatat caagggaatc gataccaatg tagaacctat tacactaaat acataaaccc
    10801 atataggatt taagcccatt gcaataccta taggaatggc tcccctcaat tctgttacag
    10861 gaaccataga taaaattaat atgtgtaaat atcccaaact attccctcct attctaagtg
    10921 acattaagtt atgaatttat ttgctctata acctcaccat cctttttact aactaaaaat
    10981 gcatttttag tcaatgaaag cattggtaaa tcactcttag agtctgaata tgcatatgaa
    11041 ttttcataat caatctctat cccaagttta cctaatacag cctttattct ttttactttt
    11101 tcaattccct tacagttttc tccaatgaat ttatttttat atcttgaatt ttcataaaaa
    11161 aactccgtgc ctataacctc gtctgcaaga ccataatact taaaatattt catatatgca
    11221 tatggagatg cagttatcat aattataaca ttatcttctt ctttattttt gtaaataacc
    11281 tttttcatag attcgttaat ttttttcatt atgcaatcat caaaaaactt ttttaaatct
    11341 tcttcactaa aatctactat tggataaaag attgcctctt ttgccttctt caatggtatt
    11401 attctaatta aatacataaa tagaataaaa ataatgtatg gtaactttat aatagtttta
    11461 aaggttttat tttttaaaga gtaaataaaa aaatctatca tgctatcagt atttataaca
    11521 gtataatcta catcaagtaa taatacactt tttttcataa gtatttcccc atcctatcac
    11581 taaaaatatc actaaaaaat caatattatt aaacatgtaa ttatccataa gattacgttt
    11641 atttttaaag cattatcttt taagacaata tcagtaggat tattttcatt tgtagtgtta
    11701 attatataat tatatctaag tacaccataa actacaaata ttgttgtaaa tatcatatgt
    11761 ggcttttctg gattacttgt agaatacaat gcataacata caattgtaga acttaatata
    11821 actatcatca tttgatttag attttctatg tcataatctt ccaatatttt tctatgttca
    11881 actgcatcat ctcttagagt ttctatttct tttttacgtt ttccaagagc taagtacaga
    11941 gataagaaaa atgtacataa aattatccaa tttgataaac taacacttat ggctacacat
    12001 cctgcataaa ccctaagtat aaaaccaaga gatattgaaa acacatctaa tagtattata
    12061 ttctttagtt taagagaata taatatattg tttatcaaat agagactgag aattatagaa
    12121 agattaaaac taacaactgt tgataaacag actgaaatta aagctaatac tacaaatagt
    12181 atattagcac tttttttgct tattcttcca cttgccactg gtctattaca ttttttagga
    12241 tgttttttat ctttatccat gtctatagca tcattcagtg catatactga tgatgacatt
    12301 agacaaaaac acacaaacgc aataaacgta tttaaaaata aatttatatt tagtatgttg
    12361 tttgaaaaaa ttatagctga aaatacaaag aaattcttta tatattgctt tattctcatt
    12421 aactttatat aatctgaaat tttagtaaga agccttatac ttgtattggt ctgtttcata
    12481 aacttactac cctttcaata ttatttttta aacaggatat gattttatcc ctatgtttaa
    12541 aatttctata aatccattga cattactgat tactatagta accaataaca gcgatagaaa
    12601 tatattatac atattctttt cattttttct aagcgctttc gtaaagtaag ccaataaaat
    12661 tataactgca aaactaaaat ttatagccat aatacttgaa ttgactagat tatatcctac
    12721 tataccatgt aaagttagat tgaatataat tattcccata catagcttag gaactctttt
    12781 ttctgaattt tcaataactg ataaaacaca aaaacataaa gctaataatg taagaattac
    12841 tggtattgta gcaacttctc cattttgcaa aaataaccct tttttaatac ttgctccagg
    12901 aaaataaaaa accgaataca ataatctctc tataatcatt gcaatctttt ccaaaattgg
    12961 ttctccactt ataaatttcg tatcatactg tattacattt ccaaggttca taaaactctc
    13021 ataagacttt gttgaaacta cacaaatata tgccattagg ataaatgtaa ttaaatgttt
    13081 taacattttt cctattttta atttaaactc aaatatcata ttaaaaaaat acaaatatat
    13141 attactaata gttattccaa atagaggaat agctgctaat tctactaaat aagaattttt
    13201 tcctttcatc ttatttgcaa taactatgaa aaatattaat gaaaattggc tatatataaa
    13261 tctctctaca tttaaagtca taaataactg tggaaaagaa aatatcatta tcatagtaag
    13321 taaaattaaa gttaatctct gcatttttat ctttttaata tgattaaaga ctaaagttat
    13381 tgacatagca ctagttatta tttgtagaca aattagtcca aacccatagg cactattaaa
    13441 atctgttgtc ccaggatttg ctattgaagt acaaaatagg tatattggat atgcaaaaat
    13501 tgatataatt gctgaaaaca atatgtgctt actattgtct tgacttatag caaatacatt
    13561 taaattgaat aaaactcctg tatctgtatc taacaataca tcataagtat tgtttaaatc
    13621 ataaaatata gtagtcttag aaaaaaatat cttagttatt attaagtaag ttattacaac
    13681 taatataaac aaaaacatct cgtttttata ttttaatatt ttactcatca atatatcacc
    13741 atccattcat tatataggat ttgctgacac ctacaattta ttccttatat ttaatagcca
    13801 tcttcataat gttcttactt gttttattgt aattgaataa ctctttattt acaaccacaa
    13861 tatttacttt attgatgtta taatcagtca agtattgttt atataacata gattagtgtt
    13921 gtataaaatg agaatttgta agcaatatta atgtaaaata tttgaaaaac tttatataaa
    13981 aatatacaat gttgcttatg gaattaaact ataacaaatt aaagcataat ttaaggagac
    14041 aaaattataa tctgtctcct taaatattaa aactatattt taaaacatta ctaaatacta
    14101 tacaaatttc aaatatagta taaaattaat tagactttaa cactattagc tccatcttct
    14161 tcccattttt taatttcttt taatctatct attataggta aatgctgagt acattcaact
    14221 tcacattttc cacaagatat acattcttta gcttttgcct tactattcat tgtgtaattc
    14281 caatgtccta agccataaac taactttgtc atttcttcat cactcttttt aaacatctgc
    14341 ttttcattat aaaaaagcat ataggctggg gtattaatac cttttggaca tactttacaa
    14401 taaccacaac ctgtacatat ttcgttcata ttcttattta atctagtctc aatatcatgt
    14461 atatcctcat ctgaataaat tctattttcg tctgcaattt tacatgcatc atctatatct
    14521 tgttttttat taaaccctat caaagatata cttatttgtg aaattcctat gttgaatctt
    14581 aaagctgatt ctatagtact ttcgccatct tttgatagaa aagaaagttc tttattatac
    14641 tttggaatag ttccaccact taatgggttc attgccacaa caccatatcc attatcaaca
    14701 gcatatttgg caccttttag cctatatgga aagtttaata tatttattcc catagttaca
    14761 ccttcaaact tattttcact tagaatatct ataacctcat ctcctggttg atgagatgaa
    14821 aatactatat ggtctatcaa tccttcttct ttacatttca ataatccttc atattgacct
    14881 ccaggtctca ttgctagttc gtaatgttcc atctttctaa tattccaaac atgataaaaa
    14941 tttattttag atacgcctaa tcgttctatt gattttttta cagcctctat agctttttct
    15001 gatgtatcac atgcttttgg cttttcttta gttgatacat aaaaatcggt cttaccttcc
    15061 tttatcattt gtctaaaggc tagtccaaat atatcttcac ttctgtcatc acaataccct
    15121 ggtgctgtat caaagtaatt aatacctttt tcatacgcat atttgactaa atcagcattt
    15181 tcttgattac ttttttctaa atcaaacctt aatcctccat agccaactac tgaaaccatt
    15241 ttccctgtct ttccatattc aacgtatctc attgtaaatt cctccttaga tatattaaga
    15301 cttaatttct ttatagaaaa tattatacca taaataattt ttataaatta tttatggtat
    15361 aatatttttt ttataaatta tattttttta taacttgtat tttataacta agttaaaaac
    15421 tataaaaagg ttcaatatgg actattactt gagcattttc attaatttct tcttgtattt
    15481 gtttctcaat tttatgtgtt aaatcatgag attgctctac gcttataaat gggtctacca
    15541 ttacatgcat gtcaatataa atagaacttt tgctacctct acttctgata ttgtgaacat
    15601 cccttacctc attaaattca aacacaattt cttttatata atcctcatct acagccactc
    15661 tatcgaccaa aattccaata gatggcctaa atattccata agccgaataa attatgaatg
    15721 cagaaataat aataggaact atagattcta ttattactgg aaatcctaac ttaacaccaa
    15781 ctaaagtaac caatacacct agagacacaa atatatcact tcttgtatgc aatgaatccg
    15841 aaatcaaaac ataactattt agtttagtcc caattctata ttcatacatg catacaaaaa
    15901 tatttataaa aagtgtaata attaaagcaa ttaaactttc tatagttata gcaggtacaa
    15961 ctggattagc tattcttaac actgcactaa gaattatctt aattgctata acaaacaaca
    16021 taccacttat aaataaactg gttataattt caaacttttt atgtccataa ggatgatttt
    16081 tatcttttgg tttagatgca aagaaaatac ctataagccc cactatatta gatgctccat
    16141 ctgacaaaga atgaaatccg tctgctgtca tactatagct ttttatttga ttaccaataa
    16201 tgatttttaa taatgctact gcaaaattag caaataaaat tatccacaat acttgcttca
    16261 ctttcttata gttatccata acaaattccc cctcccttcg attcaataaa gaaagctatc
    16321 tatcaatatc aatcaaccag acaataaaat cgtatataag cacatttact atatttatat
    16381 tcaaaattgg ttgtacttgt tcattctatt tgaaacttaa ttataaataa aaatcgtagg
    16441 tatggttttg gtatacataa aaataagaga ctgtctcaaa ataaagataa tcttcttttt
    16501 tattgttgaa atgataaaat atatttgatt tcatataaaa aagagtgcct catgaactaa
    16561 aaagttcatt ttgagacact cccttaaatt tttgatataa actattaatt ttttatacta
    16621 agccttcttt aactaagaat gaatgagcaa cttcttctgg tgatttacca agttcatcca
    16681 cttgatagtt aagttctctc atagtttctt catttatttt tcctgaaagc atattcataa
    16741 ctttttctaa ctcaggatat ttcttcaaag tatcttctct tactaatggt gctgcataat
    16801 atggtacaaa gaaattttta tcgtcctcta atatcttaag attaaacttt ttaataagac
    16861 catcagttaa aaatgcatct ataacctgag attcattatt atctaaagct gtatatctta
    16921 aacttccatt cattgctttt acatctttga attttaaacc ataatctcta gaaagcccta
    16981 caagaccatc ttctctattt tcaaactcca aagttggtga gaatgtaaat tctttaccat
    17041 atttagtcaa atcagaaata gtatttattc catacttttc agcagtttca ggcatcatag
    17101 ctaatgtgta agtattatta aatccaagag ggtcaagtaa agttaactga ttttcttttt
    17161 ccatagtatc ttttacaaca ttatatgctt catcagcatc tttaatcgga gcatgtttca
    17221 ttacatttac caaaagtgta cctgtatagt caacatacat gtctaactca ccagatttaa
    17281 ttgcattaaa cgcaacagat gaaccaccta gatttaaatt cttcttaact ttaagattcg
    17341 tatttttttc tactaattca gcataaacat tgccaagtat aagttgttct gtataatctt
    17401 tagaaccaat agttattgta tcttttttac ttgaaaaact agaactaaaa acaacgaaca
    17461 acattgaaac aacaagcact acagatatta ctttaagtgc tatataattc ttcttaggtg
    17521 cttttggatt taatcctttt ggagtaactg caacttcaat tttactaaat aaataatcaa
    17581 ctaacagtgc aattatacat gatgggattg cccctgctaa aatcatatta ttatttacag
    17641 tttgaacccc tgaaaatact aaatatccca aaccaccagc acctataaat gctgcaagag
    17701 tcatcaaacc aacagctgta actgcagata ttctaactcc acccattatt ataggaagcg
    17761 ctaaaggaaa ttgtatttta aataatgtct gatttcttgt aagaccaata cctttagctg
    17821 attctaatac tactgggtca atacttgtta tacctatata cgtatttttt acaattggta
    17881 gtaatgaata aaccactacc ataaatatgg ctggtttact tcctatacca agtataggta
    17941 caagaagacc caaaagtgcc atagacggaa ctgcttggac taagtttaca aatccaagta
    18001 caaactttct aagagatgag attcttgaaa taattattcc aagaggaaca ccaactatta
    18061 tagctattaa aatagatgta acagttagac tcatatgttg tattaaaagc tctataatct
    18121 tatctttttg taatagtata aagttaaaaa aattagccaa gaacactcac ctccatttct
    18181 aagaattgtt cacttaaaac agaaagtaaa ctacttctag taataagccc taccagttta
    18241 ttttcatcag aaataaccgg aatatatcca accgaattac gattcattac atttagtatt
    18301 tctactaaat tatcaccttc atttacatgc aaaggctcac ttgacataat atcagcaagt
    18361 aatattgact tatcatcaat atctttcata tcttttacag taacaatacc ttttaatgtt
    18421 tttgctctat ctataattag taagctatca accttactcg ttctcataat ttctattcct
    18481 tgagttacag ttctagtaga attaacagca ataggatttt ttatcataat atctcttgct
    18541 tttatatatt ctggattatc ccaaactcta tcttctccga taaaatcttt tacaaagtca
    18601 tttgcaggct ttctaagtat attttcggga gtatcatatt gtgctattct tccatccttc
    18661 ataatacaaa ttttatcagc tatctttaaa gcctcatcca tatcatgagt tacaaatatt
    18721 atagtttttt tcaattcatc ttgaagagaa aataattgtt cttgtaaaga tgttctcgtt
    18781 attgggtcta aagcactaaa aggctcatcc atcaatatta tttcagcatc agtagcaatg
    18841 gctcttgcaa caccgattct ttgttgctgt ccaccactaa gttctgaagg atacttattc
    18901 aaaaattcat cactatcaag tcctaccatc tcaagtagtt gcaacgttct tttttcaatt
    18961 tcttcaacgg atttttcctt ttttaggcgt ggaattagct ctatattttc ttttattgta
    19021 agatgaggga ataggcctgt gttctgtata acatatccta tttttcttct aagctctatt
    19081 tcattttcct gagaaattgg tttgccatta atatatatct caccagatgt aggttttatt
    19141 aatttgttta taagcttcaa agtagtagtc tttccacaac cacttgaacc aatcaaaact
    19201 accaatgtac ctgtttctac aactagagaa atatcatcta atataacatt atttcctatt
    19261 ttttttgtta catttcttat ctctatcata atttatcatc ccctttttcg aacaagcaac
    19321 cttcttcaat attataagtt gtttttataa ttatatcata ctattttatt tatgtaaaca
    19381 aagtacgatt tttaggtcac tatttgtctg aatattgcta caaatataaa gtatttatat
    19441 atagtatata atacataatg tcaaaaaaca aaaaatataa aaatattcat aaaataacac
    19501 tagcctcaat tatatagtat taaagctagt gttatttcat ttaaacaaaa ctcacatttg
    19561 gatactatcc atgtattcaa ataatgtaat atctttataa gtttcttttt tatcacttag
    19621 gtttgatatt gtaactccca atagcctaat ttgttttcca ttaacatcaa catttctcaa
    19681 caaattatct acaccacttc ttatatcatt atattcgtca atatagtgtt ctaaactaag
    19741 gcttctagtt atctttgtaa agtcctcata tttaattttt aatgttaatg tttttccaaa
    19801 cttctcagag ttttttaatc tatgacatac ttcttcacaa agttcatcaa gaatgttgcg
    19861 tgtttcttct tcatcaatat ccaaattatg acttaatgta gtctcagccc caacagattt
    19921 tctaacccta tttggttcca ctggtctatc gtctattcct ctagcaaatt tatataattc
    19981 ataacctctt tttttgaata tattttcaag ttcaaataaa tttaaacacc ttaaatcata
    20041 tccagttttt atacctaaat ttttaagagt atttgaagta acttttccaa caccaaaaaa
    20101 tttattcaca ggtaatttat ctaaaaagtc ctgggcattt tcttcggtta ttacagttaa
    20161 cccatttggc tttcttaagt ctgatgcaat cttagcaaga aacttattgt atgacacacc
    20221 agctgaagaa gtaagaccaa cttctctaaa tatatctttt tttatttgct ttgctatctc
    20281 aattgatgaa tcaatatttt ttttattttt tgttacatct aagtaagctt catctaaaga
    20341 taatggttca actaaatccg tgtatctata aaatatatct cttatttgct ctgacacact
    20401 cttataaaca tcaaatcgag gtcttacaaa aactgcatat ggacacctat tgtaagctac
    20461 agctgatggc atagctgaat ggataccaaa ttttctagct tcataagaac acgttgcaac
    20521 cacacctcgg ctttgtggat ttccaccgac aataacaggt cttcctttta attttttatt
    20581 atctctttgt tctatagatg cataaaaagc gtccatatct atatgaatta tttttctata
    20641 atgtttcatt attatcacct tttaaagtat aaatagatta attttattta tattactttt
    20701 taattatacc aaacttatgt ttctttttgg tattttttca ttattttctt tatatattca
    20761 tattttccat aaataaaacc atctataatc cattaacttc aatataattt ataaaaaatc
    20821 cttaaaaaca cttgcaaaaa ctatataaaa gctttacaat aggtattcca aggaactaaa
    20881 tttaaaattc cttatggata acagtatata tatttaattt atgggatgtt ttaaaaacaa
    20941 aagactgtct tatgcaagac agcctactga ttttattata attgtaatgg actgctcaaa
    21001 ctattacttt aaaacagccc atattttttt atataatttt aactatagta aagattttgt
    21061 gaaggatatt ctgggtcaca agttacatct ataccaagct ttctaaatgt ttgttcatca
    21121 tctctattta atatagtagt agaatgagct tgacaccctt ttaataatgc taacttttcc
    21181 atagcaaatt gtgcagttgg attagttaca gcacaaatac taagtgcagt taacacctct
    21241 tggcaactaa gtgaaacatt tctactagct aaagtctttg tttttaagtt tatgataggt
    21301 tctaatatta caggtgatat taagtgcata tcatcagata tattggctaa atgtttaatg
    21361 gcatttaata ctactgctgc agttccattc attaaatcag aacccttacc agttaatatt
    21421 gtaccatctt ctaattctaa tgcaactaca ggacaagtat cattcttgtc agcactttct
    21481 ttcaatttat tactgtagtt tctagcaggt aatacaacat ttctatcttc tggctttaag
    21541 ttaagctctt ccataatcat tttcattcta tcataagcac ctttatcaac ttgtcccttt
    21601 ttatactcac aggcagtttt aaagtatctt cttattattt cttctataga agcttttctt
    21661 attacttcat catctattat tccaaagcca actctattaa cacccatatc agttggagat
    21721 tggaaaattg attcttttcc agttatcttt tctattattt ttttaagcac tggaaataat
    21781 tccaaatctc tattgtagtt tactgacatt tcaccatacg tttcaaggtg gaatgaatct
    21841 atcatattca catcttttaa atcaactgta gctgcttcat aagctatatt caaaggatgt
    21901 tttacaggta cattccaaac tgggaatgtc tcaaacttag aatatccaac tacattacct
    21961 cttttatttt catgatacaa ctgacttaga caagtaccaa gttttccact attaggtcct
    22021 ggtgctgtga ctactactat tggctttgta gtttctatat attcatttgc tccatatccc
    22081 tcatcactaa ctatagtctc aacatctgac ggatatccct ttgttggata atgcttataa
    22141 actttaattc ctctacgttc taatttattt ataaatacag ttgtagcagg ttgtccttca
    22201 aatctagtaa ttactacact atttacatct agttcataac ctcttaaatc atctattaat
    22261 cttaaaactt ccatatcata agtgatacca aagtctcctc ttattttgtt tctttctata
    22321 tctcctgcat agacacaaat tacaacttca actttttctt tcatatgttg taatactttt
    22381 atctttgcat tttcatcaaa tcctggaagt actctttttg catgaaggtc tcccatcaat
    22441 tttcctccaa actctaaata aagtttgtca aaattattta ctctttctaa gatatattta
    22501 gactgctctt ctaaatattt tttgtggtca aatcctactt tcatcttaac acctcactaa
    22561 attaaatttc ttattgataa cagtatattt atatatattt ttttagtaaa acatcttata
    22621 aaaaaatgtt aatacttttt cactcaaagt aatattatac aattaagatt aatttttttc
    22681 aagatagttt tatggtaaat aagatatacc taatataact aataaaataa gaatactata
    22741 tttttatata taagtataat attctcacct tagcattaat ttatttcaat attttcatat
    22801 atcttttatt ctaaattcaa tacttttcta atatcttcta taccttgata aacttcttgt
    22861 ttagaaaaat tcatagcttc taattcttca tcagtttttt ccataagcat agtataaaat
    22921 tctattgcta tatactgcat attttcacta tttacactct tagctttatc aaataaaatt
    22981 tcttctgctt cgtttacatt tccctgaaaa actaatcttt ttaaagttat aagtataact
    23041 tcttcttctg tattatacac taaatctttt tcttcatatt cactgtattt ttttcccaat
    23101 aaaagtcttg caaccataag ttttatctca ttgaaaatct tatccactat agccattttt
    23161 acatctccta taaataaatt ttattttctt tttaattata tcagacttta tcgtaaaaat
    23221 tattatatgt aaatgaaata attcaaaaat aaattttaac aataaaaaaa agaaggtagc
    23281 gggggatttc cgactacctt caaacactag attgtgattg gtttggtatt ttatttatac
    23341 ccaccataag ctttattaaa tcatctaaat tgattttttt agcatccaca tcctccatca
    23401 aaaacgtcac cacattcaca aagtattaat aataatatta agaaaggtat ccatgcacat
    23461 aagtccattt cagcccaaca atcttggaat gctaagaata agaagaataa tattattatc
    23521 caccaagcac atccaccaaa tccgccttca ccaaaaaatc tatccaacat aatatgttac
    23581 ctcctagtca ttttgtatgt ggagtttcag ctcctaaaat atactatgtt taaggggtaa
    23641 tatttgctaa tgattttttt aaattttttt agatattttt aaaaaaatta ttgattcaca
    23701 ctcaatgtct taccatcact attgataact acctctccat tagaagtcaa aaatgttttt
    23761 atattcaatc tttccaatat ttttaaaaca ccttcctctg gttctttttt ctctgaacat
    23821 gtaataacag catattgggg tgaaatagac tctaaaaatg tttcagttaa tttgtcatat
    23881 cttccatgat gaggcatttt aacaaaatca tattttaaag tatttccatt tataaactct
    23941 gacaatcttt cttcctctgc atctcctgca aataaaaact tatgtgctcc atagcttata
    24001 ttagtgatta ttgaataatc atttgaactc tcgtacttgc tttttgaagc cggatgtatg
    24061 tttatttctg cactattgat tacaatattc atattatctt ttagtagcac tggttctata
    24121 tcagcttttt taagtgaatc aatatattca tcatattgct tactatcttt cttataattt
    24181 gcttgtataa tattctttac tttaacacta gataacacac tatctactcc acctatatgg
    24241 tccttatcta aatgagttaa aattaaatag tcaagtgtat ttattccttt ttcttttaac
    24301 ctatccacta tttgttttcc atttttatct tctcctgtat caatcattat gactttgtta
    24361 cctatttcaa ccaatataca gtcagacttt ccagtactta atatagtagt cttaaatgca
    24421 gtattttttg tgctactctg aatattattt tgactcacat catttttgtc actttttaaa
    24481 taagattcat tcgttgattt tatcttacta ttagtagtta tatttgttac aagcaataat
    24541 attactatta tagataatgt agtaactaaa aacaggatat cttttaatac tattttcctc
    24601 atccaaaagc tcctataaat tttatttatt acatatttat agtatagcaa ccttaactct
    24661 gtaaaataca catataaaaa gggcaaattt taggcatatt tgtggcatga taaaaaaatg
    24721 actgtaccaa aacaattatc tttctaaatt tacatcttca aattctttaa tcttatcatt
    24781 agataaaaca ctctttttag tagattgtcc attttttcta tcaaaagtat ttttagcttt
    24841 tacacctgat aaaaacttac ctgctccatt aatacaacct tcttcacaca tcattccttc
    24901 tataaaatta ccttctaatc ttcccaattt agccattgtc attgtctttt taatttgtct
    24961 tggaccagat acttttatag gttcaaaatc aaaactaagc cccttttcag caatatagtt
    25021 ctgtactgat ttggcaagtc caccagatgc tccaaatccc cttccaaaaa tcgaaccatc
    25081 atttatatct tcatttgaac attttgataa atctatatta aatgcttcaa ataatgccat
    25141 tagttcttca aaagtaagta cataatctac tgaatctttt aaacttttct ctgttgcctc
    25201 actcttttta gctgtacatg gacctataaa tacaacttta gcatcagaat ctattttttt
    25261 tactaatctt cctgttgcta ccataggaga cactgtagat gatattctat ctttttcatc
    25321 tgggaacatc ttctctatat acttaaaaaa tcccggacaa catgaatttg tcatgtaatt
    25381 atctcctgat tttattcttt caataagttc attgctttca tgaactgtaa ctgcatctgc
    25441 tccacaagaa gcttctatca tatctacaaa tcccagtgat tttatagcat tttttaactg
    25501 accatatgta atattactat ctatttgacc tgttatagct ggagctacta ttgcatacat
    25561 attttctttc tttgcaagtt tttttgcaac aggtgctatc aaactcttat cagatattgc
    25621 tccaaatgga catgctgaca tacatgctcc acaatttata caattatctt catgtatcat
    25681 tgctttcata gtgtttctat taaaatccaa agctcctgtt ggacaaacgc ttttacaagg
    25741 tcttacaact tctgatatag catcatattg acatgaactt ttgcacattc cacaagcctt
    25801 acataaatca tgatttatat atgctctccc atttatataa gaaattgccc caaatttaca
    25861 tgcttcctta catctatgag ccaaacaccc tctacaaagc tctgtaactg taaattcatt
    25921 tattggacat ctatcacatg ctgcttcaac tatatatata atttgctcat ctgtgtccaa
    25981 actaactgaa tctggattta tatctccata tttactattt ggagttaaac ttaatgcaag
    26041 ttgcgccctt tcaagaacta cttctctctc tttataaaca caacatctat atctcgcctt
    26101 ttctccttta attatttcat atggaatttt ttctatattt tcttttgtta aattttcttc
    26161 aatagatagt cttgctactg ctgttaaaac ttcatgtttt aaccttttta gttgcgtttc
    26221 aaattcaaac ataagtaatc ctccctataa atcacacctt tttgctatat ttctactatt
    26281 atgtatattg tattttttat ttatttctat aaattattat aacataggca tatactttgt
    26341 taagttatat acaaaatatt atatatgtaa tttttatttt aagtgtatat aatttttatt
    26401 ttagttgttc aataattaaa tatataaata aaaatagagt tgtccaaaag ttaaacctca
    26461 acttagaaca actctattaa caatatgtat ttttgttgct ttgtattatc tttatttttc
    26521 taaatttata ttactaaaat cttttatctt tgtatttgct aagacacttt tcttagttga
    26581 ttgagaattt tttctatcaa aagtggcttt aacctttgca cctgataaga atttaccaga
    26641 accatttata caaccacctt cacacatcat tccttctata aaattacctt ctagctttcc
    26701 taaattagcc atagtcatag ttttcttgat ttctcttgaa ccagaaatct ttacaggttc
    26761 aaaattaaca ccaatacctt tttcagcaag ataattttct atagcttttg taagaccacc
    26821 agcagctcca aaacctcttc caaaaattga tgcatcatct atatcttcat ttgaacactt
    26881 agccaagtct atatcaaatg cctcaaataa tgccattatt tcttcaaaag taagtacata
    26941 atcaacagct ccttttaatt ctttcttagc tgcctcactc tttttggctg tacatggtcc
    27001 tatgaataca actttagcat ctggttcatg actttttata aatctaccac tagctatcat
    27061 aggagacact gtagaagata tttttcctac atgatttggg tatgtttttt ctatataacc
    27121 aacaaatcct ggacaacaag aatttgtcat aaaagcttct cccttttcca ttctttcagc
    27181 aaattctaag ctttcatgaa ctgtaactgc atctgctcca caagctgctt ctaatacatc
    27241 aacaaatcct agtgatttta tagcattttt tatttgacca tatgtaacat ttgcttcaac
    27301 ttgacctgtt atagctggag ctaccaccgc atacattttc tctttttgaa ctaactttct
    27361 agctacagga gctatcaaac tcttatcaga tattgctcca aatggacatg ctgacataca
    27421 tgctccacag tttaaacatt tatcctcatg tatcatagct ttcatatttt ctctatcaaa
    27481 tcctaaggca ttagttggac aaacactctt acatggtcta acaacctctg agatggcatc
    27541 atattgacaa gcttttttac acattccaca agccttacat aattcatgat ttatataggc
    27601 cattcctccg acatgtgtta tagctccaaa tttacaagct tctttacatc tatgtgctag
    27661 acatcctcta caaatatctg ttactgtaaa ttcatttatt ggacaactgt cacatgcagc
    27721 ttccaaaata tatataattt gttcatcttc ttctatatcc attgtatcta aatctatctt
    27781 atcttgacct ggttgtaacc ccatacttac atttactctc tcagatatta tagctctttc
    27841 tttataaaca cagcatctgt atgtaggttt ctcacctttt attatttcat actgaatatt
    27901 atccattgct tctctggtaa ggttatcttc ttttccaagt ctagctattg cagttaaaac
    27961 atcatgtctt aatttgtgta tttgtgtttc aaattgaaac attttaatac cacccccgta
    28021 aatgatttta tacgatacca ttataacaca atatgtcctt ttattaatat atacataaaa
    28081 tattatatgt ttaaattt
//
LOCUS       DAMJWS010000052        28047 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMJWS010000052 DAMJWS010000000
VERSION     DAMJWS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.052"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            28..879
                     /locus_tag="PC399_002303"
     CDS             28..879
                     /locus_tag="PC399_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF2593160.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNGLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1012..2625)
                     /gene="ggt"
                     /locus_tag="PC399_002304"
     CDS             complement(1012..2625)
                     /gene="ggt"
                     /locus_tag="PC399_002304"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF2593161.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2850..4787)
                     /locus_tag="PC399_002305"
     CDS             complement(2850..4787)
                     /locus_tag="PC399_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593162.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKIIELIDKDINIEMYYKNRNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDANEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYDNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4774..5541)
                     /locus_tag="PC399_002306"
     CDS             complement(4774..5541)
                     /locus_tag="PC399_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593163.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5829..6503
                     /locus_tag="PC399_002307"
     CDS             5829..6503
                     /locus_tag="PC399_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593164.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6527..7531
                     /locus_tag="PC399_002308"
     CDS             6527..7531
                     /locus_tag="PC399_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2593165.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7643..8872
                     /locus_tag="PC399_002309"
     CDS             7643..8872
                     /locus_tag="PC399_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2593166.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8880..9554
                     /locus_tag="PC399_002310"
     CDS             8880..9554
                     /locus_tag="PC399_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593167.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9557..10789
                     /locus_tag="PC399_002311"
     CDS             9557..10789
                     /locus_tag="PC399_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593168.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11075..12340)
                     /locus_tag="PC399_002312"
     CDS             complement(11075..12340)
                     /locus_tag="PC399_002312"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF2593169.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12393..13541)
                     /locus_tag="PC399_002313"
     CDS             complement(12393..13541)
                     /locus_tag="PC399_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2593170.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNKVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13641..14186)
                     /locus_tag="PC399_002314"
     CDS             complement(13641..14186)
                     /locus_tag="PC399_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2593171.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14409..15353)
                     /locus_tag="PC399_002315"
     CDS             complement(14409..15353)
                     /locus_tag="PC399_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2593172.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            15739..16620
                     /locus_tag="PC399_002316"
     CDS             15739..16620
                     /locus_tag="PC399_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2593173.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(16681..17889)
                     /locus_tag="PC399_002317"
     CDS             complement(16681..17889)
                     /locus_tag="PC399_002317"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF2593174.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(17962..18214)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(18366..19355)
                     /locus_tag="PC399_002318"
     CDS             complement(18366..19355)
                     /locus_tag="PC399_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF2593175.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            19507..19947
                     /locus_tag="PC399_002319"
     CDS             19507..19947
                     /locus_tag="PC399_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2593176.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            20274..20501
                     /locus_tag="PC399_002320"
     CDS             20274..20501
                     /locus_tag="PC399_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2593177.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            20527..22647
                     /gene="feoB"
                     /locus_tag="PC399_002321"
     CDS             20527..22647
                     /gene="feoB"
                     /locus_tag="PC399_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2593178.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(22780..23598)
                     /locus_tag="PC399_002322"
     CDS             complement(22780..23598)
                     /locus_tag="PC399_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2593179.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            23805..24089
                     /locus_tag="PC399_002323"
     CDS             23805..24089
                     /locus_tag="PC399_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593180.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   24247..25070
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=24314..24342
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(25729..27822)
                     /locus_tag="PC399_002324"
     CDS             complement(25729..27822)
                     /locus_tag="PC399_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2593181.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         9883 a         4250 c         3430 g        10484 t
ORIGIN      
        1 ataatataaa cacaggaggt acattctttg aaaaacatta tttcagcaaa agaacttata
       61 tcaaaactag aaaaaaatga taatctagtt gtaatagatt gcagatttga tcttatcaat
      121 agaacttatg gtattgattc ttataaaaaa ggacacatca aaggcagctt tatcttagat
      181 atagacaagg atttatcatc tcctacaaaa gaacatggtg gtaaaaatcc tttacaagac
      241 cctttaatac taaaagaaaa attagaaaaa atgggtgttg ataacgacac tacgattgta
      301 acttatgatg atggagattt aaatggagct tgcagattat ttttccaatt aaaacatttg
      361 gggcttaaaa atgtctatgt actggatggg ggaataacat ctttcgtaaa ggaaggtgga
      421 gaattggaag agaaagtgaa tataccaagt tgtacaggta aggaaattag accaaatata
      481 aacaatggtt tagttgttcc aatggaatat gttaagtcta aattatacaa aaaagatact
      541 gtaataatag attgccgtgc taatgagaga taccagggtt tagttgaacc tgcttactca
      601 aaagctggtc atataccaag tgcaaaaaat tatttttgta aggatttaat aaaaagtgac
      661 tttgaaaatg gctcacttaa agatatagaa tttttaaaaa aattctttaa agatttaaat
      721 aattatgatg aagtaatttt atcttgtggt tcagggatat ctgcttgtgt taacagtctt
      781 gctcttagag aactggatat acctcacaag atttatattg gtagctttag tgactggatt
      841 agttatgatg acaatgaaat caaaactggt caagaataag gctcataatc tatataaaat
      901 cagcatatga tagcttaaat tttgtaaagg cgtcagtcat tgtagacttt cgcctttact
      961 aattatttca tatactcttt aaatttatta tatatatttt agttgataat attaataaac
     1021 tgcaatatga ccatcacatc ttgattccgt cccacaaata taactttcct gttctgtttt
     1081 aaatattatt tgacctcgtc ccatgtcgac agtatcatga attactttta tttcatgacc
     1141 cttttcaatt aatgaatcta ttatgtgctt tggcatctct ggttcaactt ctatttcctt
     1201 tccttttatc cactgccatc ttggagcatc tagcgcctct tgtggattta atccaaaatc
     1261 aatcatattt gttaacactt gaatatgtcc ttgtggttgc ataaaagctc ccattacacc
     1321 aaatggacct attgctttat cttctttacc taaaaatcca ggtattatag tatgataagg
     1381 ttttttaaat ggttttacta cattgtggtg tttagggtct aaattaaaat tatttcctct
     1441 actatgtaaa gcaatcccag ttcttggtat cacaattcct gaaccaaaat ttatgtaatt
     1501 gctctgtata tatgaaacca tatttccatc cttatctgca gtacaaaggt atactgtacc
     1561 accacaatat ggctctcctg cagttggata caaagcctct ttattgtcaa tcaataaact
     1621 tcttttttta gcatactctt ggcttagtaa ttcctgaatc tttaccttca tatgctctat
     1681 atcagttaca tatgttttag aatctgcaaa agctaatttt aaactctcta taattttatg
     1741 tatgtcttct acattttcta tatttccttc taattccaat tcttttaata tgttaagtgc
     1801 cattaaaact gttattccat gaccatttgg tgggatttca aatattttat atcccttata
     1861 ttctgtactt ataggttcta cccatgttgg ataaaaacac tctaagtcac tctttcttat
     1921 agccccatta aattttttag aatattcatc tattttatca gcaagtcttc ctctataaaa
     1981 gctttcagcc tgagtatttg ctatctcttc taatgtactt gcctgttctt cacatataaa
     2041 tatatctcca gcctctggag ctttaccatc tttagaaaat gtatcaaacc atggtttgaa
     2101 ctcttctcca ataagttctt tttcatagag ttcatatgat tttttccata ccttagctac
     2161 attaggagat actacgtatc cctctcttgc atagtttatc gctggagata agcattccat
     2221 taagctaagt ttaccatatt ttttatttaa ttctgaccaa gctgctggta ttccaggtac
     2281 agttactgct ccaaacccat attttggcat ttctttcata ttattataat tttttaattc
     2341 catattttct ggagcaaagc cactagaatt taaaccatac aatcgttttt cttcctctat
     2401 ccaaacaagg gcatatgcat ctcctccaat cccattgctt gtaggttcta ctactgctaa
     2461 tgtggcggct gttgcaactg ctgcatcaac agcatttcca ccctttttta gtatttcaag
     2521 tccagcttga gatgctaatg gtgttgatgt tgcaactgct ccatttttag catacacgac
     2581 atttcttatt gatgagtatt tatgttggta ggcattaaac ttcataaatt tcagctcctc
     2641 gtcatttttt atttgatatt ctgcttagat ttattatata acatcaatta atgtaactga
     2701 tacaaaatga tataaaatat agactttttc ctaactttaa ttttatcttt aaataaatat
     2761 cttaaactca attctagtat tttctatctg cacttaaata agttatttta ttcaaaacat
     2821 atatctaatt cttttaactt aaaaataatt tattctttta tcatgtttag agcatatttt
     2881 aaagttaaaa aataatatat aaaatatcct ataaaataaa tgactagagt tatgataatt
     2941 ggtacaagta atgaccttgg tataaacttt tgtgctatag acatagcaaa taaattatga
     3001 actgtaccta caactaatgg cattaaaaat ataactttta actgttttga tattattttc
     3061 tcaatatctt tattatttgc tccaagtttt attaacataa tatatctttc cttatcatca
     3121 tatatatttg ataattgttt aaataagatt atactacctg tacataaaag aaatactaca
     3181 gacaaaaata tgcctataaa cagcattaga ccacttaatt ttatcaagtt atcataactt
     3241 tcaatacttg atgtaaaatt aaaaggataa gtaaatttca tattttcact tactatattt
     3301 tttaatttta aagtcaaatc cagttctttt ttttcatttt ttatatcaat taatcttatt
     3361 aatctagaat ttccatgtaa ttttaactga ttaaatacgt catcttttac tacaattaaa
     3421 tcaaacgtag cttgtgaatt tatttctggc tccaagatgc ttttttgtac tttaaacaga
     3481 tttatatcat actcatttgc atctttaata tgcaagttta ttttttcaat ctcaaagtct
     3541 ttaaaaaata tttttttgta tgaatcagaa acatagtaca cagattcctt tgaagttaat
     3601 tcttcataat tgttattctg atattgtctt aattttttaa aatcactttc tctaataata
     3661 tctacagacg ttctatcttc tatataacta tttcgatttt tataatacat ttcaatattt
     3721 atatctttgt ctataagttc tattatttta tcaaagatta tcttattatc ttttttatat
     3781 ttatataata atttgtcaat ttcgttattt acataagaat ttcctaaatt tatggtataa
     3841 ctgtatttat aattttcact tatgttccta tctatatcgt aataaagtga tatggtaaac
     3901 cctagcatag ttacacttgt agctattaat attgctatta cagccaatac actactattg
     3961 ctctttaatt taaatccaag ttcagaaaat gcaattaaat tctgtccctt ataataaaaa
     4021 ctttttctct tcttaacagc atcaataatc atatacatag ttgatgaaaa aaataaaaaa
     4081 gttccaggta ttattgtaac taatataaaa actgaaagca taatattttc agcaataaaa
     4141 taactaattg acattaaata agaaatagct attaataaaa caccaagtac acattttaac
     4201 tttataaatt ttttaaactt atacttttct ggatatttat taaataactc tataattctc
     4261 ttatttctga taactatagt atttcttatt ccaataacta tgaatattat taaaaatacc
     4321 gctagtgtct gaactaaggc ctttatactt aagttcatat ttactactat catttcattc
     4381 attaatttta caagcatcat agtcataagc tttgaaaata acagtcctaa aaatatacct
     4441 attagtattg caactatacc catcaaaaaa gtttcatata aaaatatttt tcctatctga
     4501 tttttcctca ttccaagtaa actataagtt cctacttctc gcctacgtct attgaaaaaa
     4561 aatgagtttg aataatatat aaataaaaat acaaatacca ctattacaat tgaacttgta
     4621 ttaatactca tcctagtttt ctctccaagt gaactcaacg cttcattata ctctatagat
     4681 ttaaaactaa aaaatacaaa tacactaaat acaattgaaa taaagtacat taaataatta
     4741 aaaaaactat tctttacatt ttgcattgca attctaaata aagtcatttt tattaactcc
     4801 tccaattaag gaaactgtat ttactatttt attatagaac tctctgttac tttctccctt
     4861 tacaatttct gtgaatatta ttccatcctt tataaagagt attctattgc aaaaactcgc
     4921 acttatagca tcatgagtta ccatcaatat tgttttattg ctgtctttgt ttaattttaa
     4981 tagacaattc aacaaatccc tagcagattt agaatctaat gctcctgtag gctcatctgc
     5041 aagtactaat gatggttttg ttgctatagc tctacataca gctcctcttt gcttttgtcc
     5101 accagaaact tcgccagggt atttatctaa tatagattct atatttaatt cttttgaaat
     5161 ctcttttatt ctattttcta ttactgatac tctttcattt tttaaagaaa gaggcaacat
     5221 tatattttct tttattgtaa gtgtatccaa aagattagaa tcttgaaata caaagcctag
     5281 attatcacgt ctaaaatctg ccaattcatt ttgcttcatt ttaatagtat tctttccatt
     5341 tatataaata tctcctgatg aaggtaaatc tattgtagaa ataatattta aaagtgttga
     5401 tttaccagca ccggaagacc ccataatacc aacaaattct cctttataaa ccttgaaagt
     5461 tattcctctt agagcttcat gttttgaacc ttttcttcca tactcctttt ttatattttc
     5521 aacagataat atttcttcca tagttaataa tcacctttca tcacaattag ttatttatca
     5581 attctcacaa atatattata tatattaaaa tggcttttaa acatttaatt atattaattt
     5641 aaggttacat tttcgtaagt attaattata tttcttcaaa tttattgcaa ttatgtttaa
     5701 aatataattt atcaagtaaa atatagttat attaatataa ttattttagg taataaggta
     5761 gaaatatttt aatttatcaa gtagaatata gttatgttaa tataactatt ttgaattagg
     5821 aggtaagaat gtttaaaata atggtagttg aagatgacgt ctcattgaaa aatatcatag
     5881 caaagtgtct tactaaatgg ggacatgatg ttcatcaaat agaaaatctt gaaaatataa
     5941 tagaagagtt caaaaactat aacccagaat tagttttatt ggacataaac cttccatttt
     6001 atgatggttt tcattggtgt aatgaaataa gaaaaatttc aaaagttcct ataattttta
     6061 tttcttcaag aaattctaat atggatgtaa taatgggtgt aaatttaggt gcagatgatt
     6121 atatacaaaa acctttttct gtagatgtct tagtagctaa agttaatgcc cttttaagaa
     6181 gaacttataa ttttgttgat aataactcaa atcagattat tcacaatggt gttactttag
     6241 atttatcaac tgcaacaata aactatgaag ataatactat tgagctaaca aaaaatgaaa
     6301 tcaaaattct ccatgagctt atgaaatata aggggcagat agtaagtagg aataaactca
     6361 tgaaaaaatt atgggataat gattggtttg tagatgataa tacattaact gtcaatgtaa
     6421 atagaatacg ctcaaaatta aatgaaattg gtttagaaga ttttatagaa acaaaaagag
     6481 gattgggtta tataatcagt taaaacaatt ctaagtaggt gaatttatga gctttaaaga
     6541 ttttctcaag gataaactag gttttttagt atacaatttt actttcttaa tttttacagt
     6601 atcagttatt atattttctc ctgtagatgt ttttctgact gatactattt tatatatatt
     6661 agttgtaaat gttgtgtttt tatttttata tctatttatt agctacatca agaagaacaa
     6721 atttttagat aagataaaaa atgatacatt caaaattaat atgaatgata tagaattagc
     6781 tagatgtaaa aatgaagaaa aaatatactt gaacattatt aaaaatcatc aatatcaatg
     6841 taataacata ataaataata atatcgagaa atttgaagaa aacaaagaga ttttaaatat
     6901 gtgggtacat gatgtaaaga tacctatagc tataataaaa ctcattcttg aacaaaatga
     6961 aaatttaatt gatgaaaaaa tttcagatga catagatagt gaaatattta gaattgaaaa
     7021 ctccgttgaa aaaattttat atttatcaag actcgaagat tttcataagg attttttaat
     7081 tcaagaggta aatattgaaa aaatcattag aaatattata aaaaaatact caaaatattt
     7141 tatttcaaaa aaaattattt tagacttaaa taatttgaat tttaaggttt tatctgatga
     7201 aaaatggcta tacttcatat tcgagcaatt attgagtaat tccctaaaat actcctcatt
     7261 aggagataat atatctatct attgcaaaag aaccaaaaat tatttacaat taagagtgga
     7321 agatactggt tgtggtataa aaaaggaaga tttaaatcga atattcaata agggatttac
     7381 aggtaataat ggaagaaaca attctaaatc tacaggtctt gggctttatt tagtaaaaga
     7441 actttgtgat aagcttggtc ataaaattga tgtagattca gagtacaacc aatatacaaa
     7501 atttactatt acattcaaat gtgatttata gtatcttatt attgatagtt tattttcatt
     7561 aacttatatt caacttctta tagtaattct atatgtacat cccagtatat aattaagtag
     7621 tttttataaa aaggaggaag acatgtcttt tctaaaaaaa tttaaaaata aaagatttag
     7681 aaataacagt tttaaaagta acaactttag aaatagcagt tttggaagta gtggcttcaa
     7741 gtctaaaaat aaaggtttta aaaacaatcc ctttaaaaat aagagattca aaatatatgc
     7801 aggaatagca gtaatcatac ttttaattct aggagttctt gcttatgttg attcgaaaaa
     7861 cactgaatta caagctaatg aaaattttat cgaaacatat actattcctg aaaatgaaaa
     7921 aatatttata aatgggatgg ttgttcctaa gcaaactaag gacttcaata tatctggaga
     7981 ttatgagtta agtgatgtta atgttacaaa tggtcaaaaa gtaaatcaag gagaccttct
     8041 ttttactgct aaaaatccaa ctataatagc agaaatagat agtttaaaat cacaacttag
     8101 tcaatacaaa aaacaaaaaa tatcactttc tgatattgct gaaaataaag atgctattgc
     8161 ttctataaat gcacagataa ctgctttaaa ctctcagata gcttctctgg ataaaaaagc
     8221 ttatgacaga gttactgctc cttttgatgg cacagtatac ttgaatgacc aaactggaaa
     8281 tccagaccaa ccagtttcat ttatgactat tcaaggtcta gagttttata tgaaaggaca
     8341 agcaagtgaa caagatttac ctaaaatgaa gattgaccaa atggtcaata tactagtttt
     8401 ttcaactgac caaaagctta ctggtagaat aagttttata tctgataaac catctactcc
     8461 aaatactgaa atgggagctc agcagaatac actatcttat tatgatataa acatagcatt
     8521 tgataatcaa gaaggattag taaatggatt ccatcttcaa gcatcacttg aagtagctaa
     8581 ttcaagcttt aaaataccag catcttgtgt acttaaagac aacaaacact catatgtatt
     8641 taaggattta gatggtatat tgaaaaagca aattgtagat gtagcaagtc aaaatgatga
     8701 ttttgctgta gttagaggcg gtcttgaaca aggcgatata ataataaaac accctactaa
     8761 agaaatgaaa gaaggagacc cagttcaagg cgatggcgta actgctggtt ctaataatgg
     8821 agatactact ccaaacaaaa aagtagaaga agttactatg gatgtgaatt aatttacata
     8881 tgttaattaa attagaaaat attcaaaaat attacaaagt agggaaagat gaattacatg
     8941 ttttaaaatc cttaaattta gaaattgaat ctggagaatt tgtcatgata atgggtaagt
     9001 ctggtagtgg taaaactact ttacttaata tcttaggttt tctagatgta tttgatgaag
     9061 gaagatatat atttgatgga acagatgtaa ctaatttgag tgaaaatgaa cgttctgttt
     9121 ttagaaatat taatattgga tttgttttcc aacaatttaa tttaatagaa actctaaatg
     9181 tatatcaaaa tgtagagttg cctctaattt acaataaagc actaaaaaaa tctaatagag
     9241 aagaaattgt taaagataaa ttaagttctg ttgggctttt ggacaagctt aagcagaaac
     9301 cacttcaatt atctggtggt caacaacagc gtgtagccat tgcacgttgc ctagcaaatg
     9361 accctcagat aatctttgct gacgaaccga ctggggcatt agatagtgaa actagcagag
     9421 aaattatgga gcttttaaca aggctcaaca aacaaggtaa gactataata atggttacac
     9481 atgatcaaga tttaactaag tatgcaacta aggttatacg acttaaagat ggtgtgttta
     9541 cttcggaggt gtaatcatga gtttattaaa aaattctttg gccaatttaa aaggtcacaa
     9601 gcttcgtgta tttgtagctt tactctggat tataatagga ataacttcag taatacttgt
     9661 tagttctata ggtaatggat ttcaaaaaga aattaagaaa tctgtaaaca atgtaaaccc
     9721 taataaaact actatttcgt ttgaatcagc cgataacaca ggtcttactg atgatatgag
     9781 tattttttta aaacccttta atgcaaaaga tttagaagaa ttatcatttg ttgaaggagt
     9841 agaaagaata gctccttcaa gagatgggtt taaccttgat tcagtttact cttcacaagc
     9901 atcttttgat aaaaaaacta cgtatgttga tgttggacca gtaaaaaaag attctaaaat
     9961 aaatttaatt tgtggtagag atttttcttt agatgatgaa aaaagaaaag taattttact
    10021 tacattacaa agtacaagtg aaatttttga aaatccagaa gatgccttag gtcatggtat
    10081 caacataaat ggaactatat ttgaaataat aggggtatta gatgattctc agcaaaacca
    10141 agctggagga ttctttggag gttaccaaga tatgcaattt actacttccc tagtacctaa
    10201 aaaggctttt gacactttaa tgagtcaaaa ttcttattca aatgaaattt accagttaga
    10261 cttagtttct tctaaaggct ataatgtaaa tgaagttgca aacaatgtta tagctaaatt
    10321 atatgagatg caccctggta tcaatggttc ttatactaca ccagacccaa ctgaacagac
    10381 agcttatctt gaaagcatta attccaatgt aaacaaatat gtatctataa taactgtagt
    10441 tgcaatgttt gttggtggta ttggtgtaat gaatataatg tatgtatctg ttatggaaag
    10501 acaaagagaa attggtataa gacgtgctat tggagcaaaa cctaggtcta tattatttca
    10561 atttttagtt gaagctgttt ttataacagt atgtggtgga attttaggaa ctatagttgg
    10621 atttgctgct actaactatg tttctaaata tataggtttt gaagctatac ctagcttaaa
    10681 tagcttattt tatgcaattg tagctactat attgactgga gttgtatttg gtctgattcc
    10741 agcctttaaa gcttctaaac tagaccctat aaaggcaatt tataagtaga aattgtataa
    10801 agattttcga tttttagatt agtttatata aaacatactt aaatatataa cgcactatta
    10861 tatatgatag attaggaact taatctttcc aaatataata gtgcgttctt tcataaaaag
    10921 gattctcttc cttactatat ctaaattacc aagatgttat gttctaattg ttcaaactaa
    10981 tacttccaat tttgattaat caatctcatt ctcctaatat acaatctttt actttcaatc
    11041 attgtacact ccgccttaaa aaaataatct taaattaggc gttatataca atctccccat
    11101 tacatattgt ataattaacc tttccatata aagatttacc tttaaatggt gaattatcag
    11161 ctttagaaac aaagctttca actgtccatt cttcttctgg attgaatata acaatgtctg
    11221 ctacagcccc ttcttctata aatccaatat ttaaattgta caattttgca ggatttatag
    11281 acattttttc tattaactgc atcatactaa gatgattttt atgcaccaaa tttgttattc
    11341 caagagctaa agaagtttca agcccaataa ttccacttgg agcttttata aactctctat
    11401 ctttttcttc tttactatga ggtgcatggt ctgttgctat tatttcaata gtaccatcat
    11461 ttaatccctc tataatctta tctctgtccc atttacttct tagaggtgga ttcatttttg
    11521 catttgttcc aaactccaat acatcttcct cagtcaatgt aaagtgatgt ggtgaagctt
    11581 ctgcaactac atttgcacct aagtattttg caaatctaac catatcaaca gatacacctg
    11641 aacttatatg ttgtatatca actttagcac cagtctttag tgccaacata caatctctgg
    11701 ctaccataac atcttctgct acacttgatg caccttttaa tccaagttct gaagctacct
    11761 ttcctgcatt tacacctgga tttccaacta aagaagggtc ttcttcatga aaactaagtg
    11821 gtacatccaa ctcttttgcc ttaatcattg cctccataat tagattgcta tccattaaag
    11881 gtaatccatc atcagtaaac cctggtacac cagctttttt aagagcttcc atatcaacta
    11941 actctttacc ttcaaaacct ttagttatag ctgctgcttg taaaacattt atacatgcat
    12001 tttttgactt ttctttaaca taattaaaag tatcttcatt atctacaata ggattagtat
    12061 ttgccataca tataacagtt gtaaatccac ctctagcagc agatttagct ccactttcta
    12121 tatcttcttt atatgtaaac ccagggtctc taaagtgtac atgtacatcc actaaacctg
    12181 gagcaactac attaccacta gcatctatta ttttttcata ttcatcactt tcatcaaact
    12241 tgcctatttt ataaatttta ttttctttta taattaagtc aatattctca tctctttgac
    12301 ttttagggtc tattactcta ccatttttaa ttaaaatcat attatttctc ctttatattt
    12361 ttaatttata tagactcata taatattata cattatttta catacttaaa tacaaaatta
    12421 cgtttgacat caatagcctt tttaggacaa agttcatggc aacagtaaca tcttatacat
    12481 ttacttaagt ctataatagc acccttagtc cctatctcta tagtggatgc aggacaagct
    12541 tccttgcatt ttccacattt tatgcaatct tgaaatctta ccacaggctt tggtgttata
    12601 agctttgaaa taggttcatt taaaaatttt ggtagcttta cggaaaacaa tcttaaatct
    12661 ttacttacat ttggaatctt gtaatttttt ataattaaat cttttatatc ttcacctaaa
    12721 atttcaatat ctgaaaaatc ttcttgtata tatcctctct taatagcacc tctaattgta
    12781 gcaactttat ttggaagtaa acttataatc ttacatgcaa caacatctat agcatatgga
    12841 ttttgtgatg ctaataaaac tcctatttct cgagtagtac cagctgaagg tccatcacct
    12901 tccataccta ctataccatc cataattgtc aatgttggtt ttatataatc acaaatatct
    12961 aaaagtacat tttcgcaaaa caactcttct gtaggaaatc tataatgtat ctctgctttt
    13021 ttcaatccag ctatgcatcc atataagttt tttgtaccac cagtaaaagt tgccattata
    13081 tgagtcttta atttacaaag atttattata taatctgaat ctactattgg tttaataata
    13141 tccatatatt ttagaacatg tgctttttca ctatgtatct taacatctga aacatcataa
    13201 tttaacttaa tgcccagttc atttgctact tcttcaattc cagatgcctt ataaatgctc
    13261 ttaagagaac tttgattata tggaccacca gggctatctg ctattgtaat atcacaattt
    13321 aactctaaaa gtttttcaca tacaactttt acaaccatag gatgtgtagt tgtagcttct
    13381 tcaggctttt ttttcattaa taaatttggt tttataagta ctttgcttcc ttttttaaca
    13441 aacttatcta taccaccaat atcctctaaa tttttttcaa tagataactt tacttcttca
    13501 taattgtaag ttttacaact cctaacagat accgacttca tattatctcc atccttctat
    13561 cacaacagtt tatttatata atttaaaaaa ggagaaacca aagtttctcc ttttaatcta
    13621 tgttttaatt gttttaatta ttattttcca aaatgtctgt ttaataatcc taagaatgct
    13681 ttaccatgtc tagcttcgtc tttacacatt tcatgtactg tatcatgtat agcatctaat
    13741 cctaattctt tagctctctt agctaatttt aattttccat cagttgcacc atactcagca
    13801 tcaactctaa ctcttaagtt ttcttttgta tctgcaacta caacttctcc aagaagttct
    13861 gcaaatttag cagcatgttc agcctcttca aaagctattc tcttatacgc ttcagctact
    13921 tctggataac cttctctatc agcttgtcta ctcattgcta aatacattcc tacttctgta
    13981 cactcaccag taaagttagc tcttaatcct tcgattattt ctgcatctac accttgagct
    14041 actcctattc tatgttcatc agcccaaacc atttcgcctt tcatttcttc aaatttatca
    14101 gctccaactt tacatactgg acattgtgct ggtgcagcat ctccttcatg tatatatcca
    14161 catactgtac aaacaaattt tttcataatt aatttcctcc caattttata taattaaatt
    14221 tttattttta agtttaataa cttgctacaa gaatatatat accccagact ttatagctta
    14281 aacactaaaa ataaaattta tttcctaatt aattttatca ctttgaaaca ccagtatcaa
    14341 ccaagttttg atgtcaaatt tctacataat acataattta tttgattaat taggaaatag
    14401 ttgtcatatt aaatactttc taatgaagac tcctctgaat ctttaagttt cacttttttg
    14461 ctctttaaaa aagataattt agtttctgca gttatgattg caacaaatat caagatacta
    14521 ccaataacca attttgctgt aataagctcc tttaaaatta ttatagaaaa tattgtacca
    14581 aatacagcct ctgtggataa tattatagct gttctagttc catcaactct tttttgacat
    14641 atagtctgaa ataaaaatcc aattgtagta ctaaatacag ctagatacat agttcccatg
    14701 taaccagata attcagcttc catctttaat tgacctgcaa atgtttgaac tacaacagac
    14761 attaaaaacg ctactgtaaa ttgaatagct gtcaatgcca ttgggttgtt atcttttgca
    14821 aactcacttg taaagaatat atgaaatgca aatccgaatg aacatataag tgttagaaaa
    14881 tctccaaaat taatagagaa atcagcttct aaagaaagta tcccaattcc tataagagct
    14941 accaaactac ttattattcc tattttatca agttttcttc tataaagtat aaatcctatg
    15001 aatggaacta ttacaacatt agctgctgtt ataaacgcat ttttagatgg tgtagtgtac
    15061 ataagtccaa tagtttgtac tgtgaatgct agaaataaaa atattcctaa aatccctcct
    15121 gcttttaata attttttacc catattggca cgaatctgtt taaaaaacag taaattcata
    15181 attatagctg ccaagaaaaa tcttaaagtt atgatttgta gaggtgtaag cccaccgtct
    15241 aatgcaaatt gggttgctac aaagccactt ccccatataa ttgctataaa tattagtcct
    15301 atttctccaa agtatttttt caataaagct ccctcctttt tatttttttg caaaattata
    15361 aaccccctaa acagcaagag agtttttcta taagcattac tataaattaa tatagtaaat
    15421 tttttcagta ttttaaatct aaatttacat acattttgag ctcaaattta tatccactat
    15481 ttaacagtaa tatattaatc attttttgta aacacctttt taataaattt ttatattttt
    15541 tatatttttt tacctatact aaattttttt atagatattt ttaatatata tgatacttta
    15601 atattatgca aataatgtca tataagttac actttagtta tcttagttac aactttgtcg
    15661 gtttactttt tctactttct tcgatataat tataatatat ttttgataca attcgacaaa
    15721 agggggaatg ctgtaattat ggtaaaaaaa agaaaaaaga aaactgttac acttactata
    15781 cttgttgtaa tagttattgg tattttttct atggcatggg cggacatgtc tagaaaaaaa
    15841 gaaatgaaag aaactcaaga aagacttaaa gctgaaagat tagaaaaaga aagaattgaa
    15901 aaagaaaaaa atgaacctat tattggtgca aaaaaagaag ctgtaaaaga atatggatat
    15961 gacgcaaaaa tagtttggga caaacttaac aaatacgact attcaaacaa tggagaaaag
    16021 atagtctttc ttacatttga tgatggacca tctactacta atactccaca agttctagat
    16081 atcttgaaaa gacatggtgt tagaggaact ttctttatta agggtgattc tcttgaaaga
    16141 aaaggagcta atgaaatttt aaaaagaaca tttgacgaag gaaatgcaat agcccatcac
    16201 tcttatactc atgactataa aaaactatat cctaacagaa gcctaaatct cgatacattc
    16261 gtaaatgaac ttaacaaaac tgatgaagct atgaaaaaag tcttaggaaa gaatttttcc
    16321 tcaaatgtag ttagatgccc aggcgggtac atgtcatgga aaaatatgga acctctagga
    16381 aactacttaa aagagaaaaa catggcatca attgattgga acgctttaaa tgctgatgca
    16441 gaaggtaaaa agaaaaatgc acaagaatta tttgaacatg caaagaaatc ttctgaaggc
    16501 aaagaaatgg ttgttctttt aatgcatgat acttatggaa aacaagaaac agtaaatgca
    16561 ttggaccaaa ttataacata ttttaaagac aatggttatc aatttaaaat tttaatataa
    16621 taaaaaagca agtttattga atgtacattt tatactcaat agacttgctt ttactttact
    16681 ctattttact agctcgattt ttacaaattt tttctttcct atttgaacaa ctaattcact
    16741 ctcttttaca atagtgctta aatcctctac tttttcacca tttaccttta ctccaccttg
    16801 agaagctaat cttctaactt cactcttact cttaacaatc tcattagaaa ctagtacttc
    16861 gattaagtca aatccatctt ccttaacttg aattgtttgt atatcttcag gtatttgacc
    16921 tttttggaat acagatttaa atctttcttc agcttcttta gcagattctt ctccatggta
    16981 taatgttact atttctcttg ctaagttcat ctttatatct cttggattta ctgaaccctc
    17041 tttcaactga gattctattt tattaacttc atcaggatga acatctgtca ctaaattgta
    17101 gtatttaatt ataagttcat caggaatttc catagatttt tggtacatta ttcccgcttc
    17161 ttcatctatt ccaatatagt tacctaaact cttactcatt ttttctttac catctaatcc
    17221 ttctattaaa ggcatcatta ttactatttg agactccata ccaaattctc tttgtaatga
    17281 tcttcccatt agtaaattga atctttggtc agttccacca agttctatat cagcttctaa
    17341 tgctatagag tcatatgctt gcattaatgg gtagaagaac tcatgtactg atattggcat
    17401 ttgtccttca tatctctttt taaaatcttc tctttctaac attctcgcaa cagtaatagt
    17461 tgctgctaat tttattacat cttcaaaatt taacttagct aaccactcgc tattaaatct
    17521 tacaatagtt ttttctttat ctaaaacttt gaaaatttgt tcttcataag tttttgcatt
    17581 tgcaagaact tgctctgttg ttaaagcttt tctagcttta gattttccag ttgggtctcc
    17641 aatttttcct gtaaaatctc caattattat tactatttga tgacctaaat cttgtaattg
    17701 tttcatcttt ctaagaacta ctgtatgccc taaatgtata tctggagcac ttgggtctag
    17761 acccaatttt actatcatag gctttccttc tttttctgat ttaataagct tttcccttaa
    17821 ctctttctca tctattaaat catctacacc tttcataatt attcgcattt gctcgtctat
    17881 cgatttcatt tttcattcct cctaaaatat ttatataatc tataattttt atttttataa
    17941 taaaaaaagc tttcatcctc ttaaaaggac gaaagcattg cttacgtgtt accaccttag
    18001 ttcatcaata tctcacgata ttaatctctt agagtacaat tacatatact cgtgtactat
    18061 aacgtgtact aatccgatat ctcttaatgg ttgttatctt tcagagatag tgctctgaga
    18121 tgtattcaaa ctaaatcaat ttattccatt tcaccaaact ggaactctct gttaaataaa
    18181 atctagccct acttcttctc ttcatagcat ttatatattc agattatttc atcaattaca
    18241 agaaattttt cttaacttac tcataatata ttattatttt tatattttgt caaccttttt
    18301 tattattata tacatgtttt tttacattat actacttttt tatatttttt accaataccc
    18361 aaaatttatt tttcaatcaa aagatttatt tgtttattga ctatattact ctgactcaat
    18421 cttatattta ttttcttatt taaaaatgcg tttataatta ttaaactaat aactgttaca
    18481 tcaaatataa aaaacagcaa taaaattatt atcaaaaata aaattctagc atataataat
    18541 tttctaaact tagctattat aaagttttgt agtgacatac atgagtctct aatgataaaa
    18601 tagacactta tataggttat tgatataaag attgttttta aataaacaga atcaacttta
    18661 acattcaaca gttttagaac ttcaaaaatg gctagataag aaacacatat gtaaaaagct
    18721 ttttttgagc aacataaaaa ttggtttaaa tttctaaagt tcttaaatat taaaaatttt
    18781 tttaaagaat tagaatctaa aatatgcaat ttattcccta aaattaaact taataaatat
    18841 atgcaaaaca ccataccaaa aggaaacaat gctatcatta aataataaat aaaatttgta
    18901 tacccattaa aataaatcca tttgctatat ccttgaaatt ctttcataaa actataacct
    18961 attaatgttg aagcatatat atcaatgaaa accattacaa taaccccaaa tactgaacca
    19021 tatactaaat catccataac cattgtaggc ttatttatta gatagccaca cattatacca
    19081 agtaatacac tctgacttgc ccaaatagca gttcctagat ttccaagtac cagagaaatt
    19141 attaaaagcg aagtaattcc agataaaatg gtatacttta aatcacattt caagcagatt
    19201 atacaaaaaa atactggcac tataaaatct aagtaaatag catatccaaa tccagtactc
    19261 acagctataa ttgttactac tatgaataaa gatgacaata aagctgcttc agttaatttt
    19321 ttagtagtat tcaaaacact gcctccataa atcataaatt ttctaactaa agttttgttt
    19381 ttaatatagt atatcacttt ttaaaaatat ttttaataaa tataaatttt aataaaggta
    19441 tttatgttaa aaatggtata atgtatctta atattcagtt ttttaagatg ctaggaggta
    19501 attactatgt ttaaaattaa acactttaat gatttgagtt tagatgaatt ttatgaaata
    19561 gcaaaatcaa gatatgaggt atttgcttgt gaacagaaga tattttcatt aaacgattat
    19621 gatgatatag ataaaagctc ttatcacata tttttaaaag aaaatggatt gatttgtgct
    19681 tacgctagaa taattcctaa agaatacagc tcttataatg atgtttccat tggtagagtt
    19741 ttagttcttt ctagtcatag aagaaaagga ttagcaaaac aaatgatgga ttgtgctata
    19801 gattttataa aagttaactt acacgaaaat aatataacct tatcagcaca aacttatata
    19861 aagaaccttt acttatcttg tggttttaaa gaaatctcag aagtatacga cgaagcaggt
    19921 attgaacata ttaaaatgag attatagtgt tgacaagcta atattgttaa tatataattt
    19981 aagtataaat taaatattga attgatattt aggtatttga aaatacctta catagagtca
    20041 gaaggctttt gctttctgac ttttttatta tcaaaattca ataattatat ggattcaaag
    20101 tgttaatttt tattctatat tctactttcc ccaaaagtaa gaattatacc caactttgta
    20161 tgataggcat cttgcctatc ttttttttgt aaaagtaaca aggtaataat ataatgcata
    20221 gtttaaaata agaagattag cccaggctaa atttcaagca tggaggtgct attatgaaaa
    20281 accttaacga tattcaagtg aaaaaaactg tcaaagttga agatatatta tctagtggta
    20341 atttaagaga aagaatgtta gcgcttggtc ttactagagg agctgttata gatgtagtta
    20401 gaaaaggtcc aaaaaataat ttaactgtct ataaaatcag aggtagcaag atagctctac
    20461 gacaagaaga aagtagctta atattagttt ctgacattta aaaatttcta gcaggaggat
    20521 attaatatgg ggttgacaca taactcaact aaaatgagtt ctttaaaaga catgtttgat
    20581 atagacaata aggaagacca gtttgtaatt gcattggctg gcaatccaaa tacaggtaaa
    20641 agtactgtat ttaatcatct tacaggactt agacagcata ctggaaactg gccaggaaaa
    20701 actgtagcta cagctcgtgg taattttaaa tataaaaata ccgaatatgc attaatagat
    20761 ttaccaggaa catattcttt atttgcctta tcacaagaag aaattgtagc tcgtgatttt
    20821 atatgttttg gtaatccaga tgcagtaata gtagtatgtg atgctacatg tcttgaaaga
    20881 aatttaaatt tagtatttca agtgatggaa cttacagata aagttatttt gtgtattaat
    20941 ttaatagatg aagctagaaa aaaaggcatt actattgata aaaaactttt agaggattct
    21001 cttggtattc cagttatttt aactgctgca agaaatggct ctggtatgga tgaactattg
    21061 gatactttaa atgatgtatc ttttgacaaa tataaattaa ataataaacc tgttagatac
    21121 aatgaaaata tagagaatgt ggtaaaatca attcaaccag aattggataa tattatacca
    21181 ggtataaatt ctagatggtt gggacttaga cttattgatg gcgatgaaag tatatttgaa
    21241 tctatgagta attatataga caaagattct atagatgcta ttaatgaagt aaagaaaaag
    21301 attccagaca atataaataa acagaaaata agagatgagt ttactaaaat caactatgat
    21361 tatgctaaaa aactgagtga tgagtgttgt tctaatgttg ctaaaaaatc aactgataga
    21421 gaagaaaaag tagataaaat attaacatct aaaatatttg ggcttccaat aatgctctta
    21481 ttattaggta ctatattgtg gattactata gaaggagcta actatccatc cactttatta
    21541 tctaatttat tactcggatt tgagccaagt atttcgggta tattaaatag tattaattgt
    21601 ccatcatggt taaatgatat gttggtttta ggattataca gaacattagc atgggtaatc
    21661 tctgtaatgc tacctcctat ggcaatattt tttccattat ttaccctact tgaggatttt
    21721 ggatatttgc caagagttgc atttaactta gaccacctat ttaaaaaagc ttgtgcacat
    21781 ggaaaacagt gtttgactat gtgtatggga tttggatgta atgcagctgg agttattggt
    21841 tgtaggataa ttgactctcc aagggaaaga cttatagcta tacttacaaa caactttgtt
    21901 ccttgtaatg gtcgttttcc tactttgata gctatatcta caatattttt tagctcagtt
    21961 ataaccaact ctttcgtatc aagcgtagca actgcacttt gcataacttt actgataatt
    22021 ctaggtgtta taataacatt gttggtttct tatactcttt ctaaaacgct attaaaagga
    22081 gttccgtcta catttacatt agaacttcct ccttatagag ttcctcaaat aggaagaact
    22141 ttatatacct ctataataga tagaacaata tttgttttag gaagggctgt aatggtagcc
    22201 atacctgctg gtgtcataac ttggatattt gcaaatatat atataggaga tttaagtata
    22261 ttatcacatg ttgcaaactt tttagaccct ttagctaaat taattggtct tgatggtttt
    22321 atattacttg cttttattct tggattccca gcaaatgaga tagttgtacc tatactttta
    22381 atggcttacc tagcaactgg ttctatgata gaattagata gctttagtgc tttaggtcaa
    22441 gttcttagag aacatggatg gacttatttg acagctttaa atgttatgct gttcagttta
    22501 ctgcattggc cttgtgcaac aacactttta acaataaaaa aagaaactgg tagtttaaaa
    22561 tggactgcct taggattctt aatgcctact atacttgctt ttgttgtttg ttttttaaca
    22621 actacagttt acaatttatt tatataatta gtattaactg acagtagaca tatattagag
    22681 aaaccattta aaaatttata tactttatta taaagcatag agatatattc aatgataagt
    22741 aaaattactt aacatttgaa tatatctcta ttttaattct tatctggata cttggattga
    22801 aatttcagtc atataattat cttcattact ttcacaaaaa aagtcaagta tactctcctc
    22861 atacgaattt ccaacaattt tatattcatt ttcatatata tattttttca atttttcgta
    22921 agatttatat gaatcctcat attttccaat atgttttatg caagcataca ttccttctgg
    22981 ctttacatat agctttttat cctttatttt tttatctatc ttcaaataaa aacttgatat
    23041 acttaaatag tccccatttt ttatattttc attacttact attactccca actcatatcc
    23101 ttgatacaaa tttttcgatt tacaataatt ggtaaaatct attaattcca tcataaaatc
    23161 actattactc atattactca aatcgcttga tagaactaaa tattcttcaa actttttgga
    23221 tatataaatt tcttcactat tcattttaga aattccttca actgttatat ttattctatt
    23281 ttgcatctta gaacttatct ttcttagttt ttttattttt tctcttatct ttttttcttc
    23341 ttctttaagc attttcacac aaagctgggg agttctattt tgtatatact cttttatttc
    23401 ctctaaactc attcctattt ctcttaatga ttgtaatata tcaagtgtat catattgata
    23461 aactgagtaa tatctataat tatttttatc tttatattca ggagaaaaaa tacctatttt
    23521 gtcatagaaa attaatgtct gcttagatat accacacaat ttagcaaatt cacctgttgt
    23581 aaaatactgt ttttccaagg gttatacact ccttttcttt ttgttgtcta tatagttact
    23641 atattcttta ttatttacta aatcaagtag ttattatttt agaaaatcaa tatggttttg
    23701 attttaaagc ttaaatatca gttagatgtc agttataaat attttaccca ttcccatcat
    23761 aaaaaagata tcccctctta acttttggat ggaatattat atttatactt gttagagtga
    23821 aaattgtaat aggtgctaca ataagatatt taattaaaca taaagttaat tattgtaata
    23881 aaaaatcttt agataaatta gagttaaagt atggaaatat agatagagaa aaagctgtta
    23941 aactagaaat attttatcaa tatttaatag aacttgaata tatagaaatg agtttattaa
    24001 taagaagatt agatactgca ataacatcat tagcattagt atctattata acaatagcat
    24061 catattattt aatcagaaaa aatgtgtaat tatgtaagct atttataata aaacactgat
    24121 aaaattaata ataaaaagat aataggattt ctatactacc tttttgtttt tataatatag
    24181 tgtaatttta ttatattttt gtatgctaat ataactagat aatcataaga taacacatta
    24241 aaagaaaatt atattctgta tagttagtat aaaacgtaag taatataaat ttatgtaaag
    24301 caacaaaaga tggatttaca ttccatatag ttaatataaa acgatttcaa atgattagaa
    24361 taaaaatatc agaattgcta tttatattcc atatagttaa tataaaacta taaagtgtta
    24421 cttttattta ttttattttt tacataattt acattccata tagttaatat aaaacgcatt
    24481 atatggctgt gtcattttgc attctattac atatttacat tccatatagt taatataaaa
    24541 cctgcttaat gcagaattaa gagaggaagt gttataagat ttacattcca tatagttaat
    24601 ataaaactgg ttgttcctgc tccggtgttg gtggaactgg tacatttaca ttccatatag
    24661 ttaatataaa acgaatatgt atggataatt ttttacttaa catattagca tttacattcc
    24721 atatagttaa tataaaacct gctgtaattt ctgatgaagc ttctccacta ccaccattta
    24781 cattccatat agttaatata aaacccttct ttaactctta tcgtatttaa acctcttata
    24841 acatttacat tccatatagt taatataaaa ctttttgtgt taagaagaaa aaagaaattt
    24901 tgataatatt tacattccat atagttaata taaaacctta aagaaaaaat ggaggaagat
    24961 taagtgaaaa aattatttac attccatata gttaatataa aactggattt aaataatcaa
    25021 aacaactccg gtttaaaaaa catttacatt ccatatagtt aatataaaac cccaaaataa
    25081 acttagtatt ttcaacactt acacgtatac aactctctta aatttgcagt gaacgagcaa
    25141 tagtgcaatt gataacgctt atcatacact ctcaacgcct tatatttcaa ctgttacgcc
    25201 aaattttttc acaaatatcg ctcactgcaa aattcctata attttattat atcataaata
    25261 tattactttt gaatattgat accaatttat accataataa aagcaaaaaa acgtaatcta
    25321 tctacattag atttaaattc tataacttaa agttttttac ataatattta tgcttgttta
    25381 atcaatctaa tatatcattc ttgtatattg cttttaatag tcttttttct taaataaata
    25441 tctatctgaa caagcatagt ttaattaaaa acttttgtat tcccttaaaa ctgtatctac
    25501 ctcttattat tttatttatg atatattatt tttaatctaa aaatagatat ctatttttat
    25561 tatggttatc cctttttaaa atactttaat catatactat tcccaattta acatccattg
    25621 aaatgctatt ttcttatatt gcaatttata attgatatat cttcaatata ataatttaat
    25681 gtatttttga ttttaaagct tttcagtgat attattgata aattagaatt aattatctag
    25741 ctttaaaagg tcaaagattt ctgttgctgt ctgcggtttt ccaaataaat atccttgtat
    25801 gtaatctggc tttatttgtt taactaaatc atactcttct tgtgtttcta caccttctaa
    25861 acatactttt attcctacat catgacaaat tgcaacaata aaatgtataa atgtagcatc
    25921 aaacttactc tttaatatat ctttaacaaa tactctatca attttcacta tatctattgg
    25981 agaaaatttt aaaacttcta gagacgagta tccagtacca aaatcatcca tagcaatata
    26041 aattcctaaa tcttgtaaag ccttaaattt aaactgtaaa atactcatat tttgtactgt
    26101 atgactttct gtcaattcta aaacaagatt ttgataagga aaattttcat catctataat
    26161 ttttactata tcttcaataa atgtgtcttc taatatttga acagctgaaa cgtttacact
    26221 cactttaaaa aaaggatagt aatcaatcca tttcctaaaa gtacgtaatg ccattcgaag
    26281 cacccaagcc cctacaatat ttatcatatc attttcttct aatattggaa taaattccaa
    26341 aggagaaatt gctttgcatt tgtcattagt ccaccttaaa agaacttcta cacctattat
    26401 tttatgactt tctgtatcta cttgtggctg gaatcgaaga ctaaaccccc taaaatcatc
    26461 attaattgat gcctttaaat cccgcatcat ttctaatgaa tacattttat tttttaatat
    26521 ttcttgggag aaaaaaacaa gcctgttttt accatgctct ttcgcatatt gtaaagagta
    26581 actagcacac ttataaagtt cttttactgt atctccatgt tttggatata ttacacaacc
    26641 agcagaaatt gttatattta atccatattt tctccatagt tgaaggtgta taatcatatt
    26701 ctgtatttga ttatatagag ttagaatttc attttcttca acattatcaa caagaatgcc
    26761 catttcgtcc ccatcaagtt tataaagttc agcattatct ggtaaaatag attgtataga
    26821 ctgtgctagt gtttttatta tattatctcc aaaatcccta tcatacatct cattaacatt
    26881 tttaaaatcg tcaatatcta ataatactat gcataatttt tcaatcatag gatttgaaat
    26941 tttatcttca aatacactca taaattcatg ataattcagt aactgtgtca atgaatcaat
    27001 cttattttgt ttacctaatt gcgtcattat tcctgcaaaa atacttggtt ctccaaattc
    27061 atcacgcatt agttgacctc tacatcttaa ccatatatat tctccactac gatttttagc
    27121 cctaaattcc actgtatgat aatccatctg gtttttacct atttcagtgt ttgacttata
    27181 aaaacgattc cagtcttctg gatgtacaat ttttttccag taaacaagcg gattttcaac
    27241 aatttctcct ggtaagtcaa ataattcaac ttgtgatggt gaatatcgaa atacgcctgt
    27301 tttcatattg caaatgtata tatattcatc tgtacctcta atcaaagcat cataaagcaa
    27361 tgtagaatta taatcaaaat tgtctcttga aaaattaaaa actccttttt cttctttatt
    27421 atttgatatt tcatgaaatt cattaatctt aattggttta tttaagcttg ttgagtttgc
    27481 aatatctatt gattgatgca tatgaacaac tgtgccatct tgccatgtaa ttaaactatc
    27541 ataattttca aaaacacgat ttaacttatt acatttttca taccacttta ttaatactcc
    27601 ttttttatca ttctttaata attctaaaac tttacaaaaa ctacaagtag aatttttttc
    27661 aggatataat acttgccaac aaacttttcc ttctggatct aaaatattat attcttcttt
    27721 catttttttg ttcataaaaa taatttcatt agtatgaata tttgtaacat atatattaat
    27781 ctgtagttga tttaatataa cttcaaaatt atgtttgttc atatacttat ctcctccata
    27841 gttactgctc tttataacaa cattgttaaa attattcttt ttagaagatt tacttataac
    27901 ttatacatat acactaaata ccttaattta attagaaagt ttattacttc atattttaaa
    27961 ttataattga taaaaaaaaa tacagcaata tagtattacc aatttaaaaa aaattaatat
    28021 tacataatac atctctctct tatatta
//
LOCUS       DAMJWS010000053        27687 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMJWS010000053 DAMJWS010000000
VERSION     DAMJWS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.053"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            280..624
                     /locus_tag="PC399_002325"
     CDS             280..624
                     /locus_tag="PC399_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF2593182.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPVRFDVIEVYL
                     LEKRINHIMNAF"
     gene            655..2256
                     /locus_tag="PC399_002326"
     CDS             655..2256
                     /locus_tag="PC399_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF2593183.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2275..3375
                     /gene="dprA"
                     /locus_tag="PC399_002327"
     CDS             2275..3375
                     /gene="dprA"
                     /locus_tag="PC399_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF2593184.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3408..5495
                     /gene="topA"
                     /locus_tag="PC399_002328"
     CDS             3408..5495
                     /gene="topA"
                     /locus_tag="PC399_002328"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF2593185.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5660..6445
                     /gene="codY"
                     /locus_tag="PC399_002329"
     CDS             5660..6445
                     /gene="codY"
                     /locus_tag="PC399_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF2593186.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6552..7817
                     /locus_tag="PC399_002330"
     CDS             6552..7817
                     /locus_tag="PC399_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF2593187.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDVEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDSHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7848..8618
                     /locus_tag="PC399_002331"
     CDS             7848..8618
                     /locus_tag="PC399_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2593188.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMIFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8727..9173
                     /locus_tag="PC399_002332"
     CDS             8727..9173
                     /locus_tag="PC399_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2593189.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9191..10384
                     /gene="nifS"
                     /locus_tag="PC399_002333"
     CDS             9191..10384
                     /gene="nifS"
                     /locus_tag="PC399_002333"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF2593190.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLDNRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10438..10878
                     /gene="nifU"
                     /locus_tag="PC399_002334"
     CDS             10438..10878
                     /gene="nifU"
                     /locus_tag="PC399_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF2593191.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11167..12246
                     /gene="mnmA"
                     /locus_tag="PC399_002335"
     CDS             11167..12246
                     /gene="mnmA"
                     /locus_tag="PC399_002335"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF2593192.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12389..12627
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12695..15334
                     /gene="alaS"
                     /locus_tag="PC399_002336"
     CDS             12695..15334
                     /gene="alaS"
                     /locus_tag="PC399_002336"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF2593193.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15359..15616
                     /locus_tag="PC399_002337"
     CDS             15359..15616
                     /locus_tag="PC399_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF2593194.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15626..17797
                     /locus_tag="PC399_002338"
     CDS             15626..17797
                     /locus_tag="PC399_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593195.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSCASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18003..18428
                     /gene="ruvX"
                     /locus_tag="PC399_002339"
     CDS             18003..18428
                     /gene="ruvX"
                     /locus_tag="PC399_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF2593196.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18506..18793
                     /locus_tag="PC399_002340"
     CDS             18506..18793
                     /locus_tag="PC399_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF2593197.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19075..19533
                     /locus_tag="PC399_002341"
     CDS             19075..19533
                     /locus_tag="PC399_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2593198.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19650..20387)
                     /locus_tag="PC399_002342"
     CDS             complement(19650..20387)
                     /locus_tag="PC399_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2593199.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20604..22280
                     /locus_tag="PC399_002343"
     CDS             20604..22280
                     /locus_tag="PC399_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF2593200.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22488..22688
                     /locus_tag="PC399_002344"
     CDS             22488..22688
                     /locus_tag="PC399_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF2593201.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22742..23905
                     /locus_tag="PC399_002345"
     CDS             22742..23905
                     /locus_tag="PC399_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2593202.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLT
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24116..24538)
                     /locus_tag="PC399_002346"
     CDS             complement(24116..24538)
                     /locus_tag="PC399_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF2593203.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24559..25395)
                     /locus_tag="PC399_002347"
     CDS             complement(24559..25395)
                     /locus_tag="PC399_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2593204.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIILDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25693..26289
                     /locus_tag="PC399_002348"
     CDS             25693..26289
                     /locus_tag="PC399_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF2593205.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            26319..27038
                     /locus_tag="PC399_002349"
     CDS             26319..27038
                     /locus_tag="PC399_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF2593206.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27040..27579
                     /gene="scpB"
                     /locus_tag="PC399_002350"
     CDS             27040..27579
                     /gene="scpB"
                     /locus_tag="PC399_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF2593207.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT        11214 a         2901 c         4992 g         8580 t
ORIGIN      
        1 cggatacaat gtattttcaa catttttcaa atagcttaca actaatataa ataaagataa
       61 atatcgtata aaagcaacta atttttctct gttcattaaa ggcactcctc caaaaaaata
      121 aacttttact atgaaaacta gaataatatc ataaaagaat aaagtcaata aaagtattta
      181 ttaatattga tggaaataaa atcaatccaa aaacttgaat agtaaaattg tatactgaca
      241 ataatttaaa agtgaaaatt taatgtattg gagtgaataa tgaataataa agaaaaaggt
      301 gattttggag aaaaagtagc agttaattat ttgctatcaa aaggagcaaa gatactagaa
      361 aaaaattaca gattaaagat aggggaaata gatataatag caaaaatgga agatgaaatt
      421 atatttgtgg aggtgaaatc aaggtctaac ataaagtttg gctacccttg tgaatctgta
      481 agcttcaaaa aaagaaaaaa gataattgga gttgcaaatt attatattat aaaaaataac
      541 ttaaataaca ctcccgtaag atttgatgtt atagaggtat atttattaga aaaaagaata
      601 aatcacataa tgaatgcttt ttaaaaaaga taatgttttt ggaaaggatt aaatatgtta
      661 agtattatta attcaagcaa cttagtagga atcgacagct ttttagttaa ggttgaagta
      721 gatgtaagca atggaatacc tagttttaat attgttggtc ttccaggcaa agaaattaaa
      781 gaagcaagag aaagagtaaa gtcagcaatt cttaatagtg gatataaatt tccaagcacg
      841 agaatagttg taaatttatc accagcagat ataaaaaaag aaggagcttt tttagattta
      901 tctataagta taggcctttt aagagagtta ataaaaaaag atgaaaatta cataagagaa
      961 agtatgttta taggagaatt atcattagat gggaaaataa gaaaagtaag aggtatattg
     1021 cccataatta tgggtgcaaa aactcagaac ataaaacgta tttttatacc aattgagaac
     1081 ataaaagaaa gtctatttgt agatgaaatt gatataattc ctattaaaag tttaaaagaa
     1141 tgtgtcgatt ttttaaacga agagataaaa gtcgataaag taaggataat gtcttttttg
     1201 gatgataagt caagaaaaga gaacgaagaa ttagaaaaag ataatagtta tatagattgc
     1261 aaatatacta agattaataa tgaagaaagt aaatatgatg aagactttaa agatgtaaaa
     1321 ggaaattatt ttgtaaagag gagtgcagaa attgctgctg ctggaaatca taatatgttt
     1381 atgattggac ctccaggctc aggaaaaact atgattgcaa aaagagtaag gacaatttta
     1441 ccagatataa gtgtagaaga aatgatagaa gtaagtaagg tctatagtat attgggtatg
     1501 ataaatgaaa ctaaaggaat aattgataaa agacctttta gagcacctca tcatacaact
     1561 acaaagcaat ctttaattgg aggaggaatg gatgcaagac ctggagaaat tgctttagca
     1621 catagaggta tcttattttt agatgagata gccgaatttg atagaaaaat acttgagact
     1681 ttaagacaac ctatagaaga tggatatgta aatatatcca gagtaaagta cagtgcaaaa
     1741 tatccttgta gagtactgtt agttgcagct atgaatccat gtccatgtgg ttattatatg
     1801 tcagagacag agtgtagatg tagaagtaac gaaattgata gatacataaa taaaatatca
     1861 ggtccattgt tagataggtt tgatattttt gtagaagtta attctataaa atattcagat
     1921 tttaattctt taaaacaaga agaaagctct caaaaaataa agagaagagt agaaaatgct
     1981 agaaaaatac agataaatag atttaagaag gataatataa aaaataatag tgaaattaaa
     2041 gcttacaatt tatttaaata ttgtaaactg gaaaaagaag cttcaaaaac ggctgagatg
     2101 atatttaata aatacaacct aagcagtaga agttatacaa aacttttaaa aatggcaaga
     2161 acaatagcag atttagaaga aagagacttg ataaattcac agtgtattat agaagctttt
     2221 tcatttagaa aggcttatta tagttatttt aaatagtttt ttgaggtgga ttttatggaa
     2281 aaaagagata cttatttatg gttaaaatca attggtggaa taacaacaaa aacaattgaa
     2341 ataatagaaa atgaaatagt aaatattgaa gatatatttg atttttctga aaaagaaata
     2401 tataatttaa aaaatataag cttgaatatt agaaaaaata tagtaaaata tagaggtcac
     2461 gcttatttag aaaatataaa agaactactt tacaaaaaag cgattaaata tatttgtaaa
     2521 tatgataaag aatatcctga aaatttaaag aatatataca acgctccaaa gttattattc
     2581 tacaaaggtg acataggctt agttaataat aattttaata tagctatagt aggttcaaga
     2641 aaacctacag cctatggaat taattgtgca aaaacaataa gttgtcaact atcccaatat
     2701 ggagttaata tagtaagtgg attagctata ggaatagatg catactcgca cataggttgt
     2761 atgagtggaa aatctaaaac tatagcagta cttggttcag gagtagacaa tcctcttcca
     2821 aaacaaaatt tacatttatc aaataaaata ttagaaaatg ggggattatt attatcagag
     2881 tataatatta actcgacagt agccccgtac catttttcta atagaaatag aattatcagt
     2941 ggtttaagtg atggagtagt tgttgtagaa gcagcaataa aaagtggtgc actaatcaca
     3001 gtggattttg ctcttgaaca tggaaaaaat gtttttgcaa tacctggtaa tataaattct
     3061 caaatgagta gaggttgtca taaaattata aaagaaggtg caaaattaat tgaaaatata
     3121 gatgatattt taaatgaata taacatattt aatattattg ataaaaaaat aaatcaaaaa
     3181 tatgataata taagtctaaa tgccaagtct aagcaaataa ttgaagctat aaaaagagaa
     3241 ggaaacttgc atatagatag tatttgtgat tatactggta tagaaataaa gtatgtaaat
     3301 tcaataatca atgaattagt actaaatgag ttagtagtag aaatgaataa taaaacgtac
     3361 agtttaaatg tataaattaa gattaggtag tgggggtgta tggttttatg gccaaaacat
     3421 tagtcatcgt agaatcgcct gcaaaagcta aaactataga aaaattttta gggaaaagtc
     3481 attacacagt taaagcatca gttggtcatg tcagagattt acctaaaagt aaattaggtg
     3541 tagatataga aaataatttt gaacctcaat atataaatat aagaggaaaa ggcgatgtaa
     3601 taaaagaatt aaaaaaagaa gctaaaaaat ctaaacaagt atatctagca actgaccctg
     3661 atagagaagg tgaagctata tcatggcatt tagctcatat actaaattta gatgaatctg
     3721 aggattgtag aatagagttt aatgagataa ctaaggatgc aattaaaaaa gcaattaaac
     3781 atcctagaaa tattgatata aatttggttg atgcacaaca ggcaagaaga gtgctagaca
     3841 gacttttagg ttatcaaata agtcctatat tatggcaaaa ggttagaaaa ggtcttagtg
     3901 caggtagagt gcaatctgtt acaacaaagt taatttgtga cagagaaaaa gaaataaagg
     3961 catttattcc aaaagaatac tggacaatag atgttgaggc taagacagta aaagacaaag
     4021 atataacttt gaaattttat ggtaaaaatg atgaaaagat tgaattagaa aatgaagaag
     4081 ctgtaaatga aatactaaaa gaaatggaag gtaaagattt aaaggttgag aagatagaat
     4141 ctaaatcaag acgtaaatca gctccaaaac catttacaac aagtatgctt caacaagaag
     4201 gagcaaataa gttatttttt actactaaaa aaacaatgat gatagcacaa gaattatatg
     4261 aaggtatcga catagaaggt gaaggtactg ttggtcttat atcatatata agaactgact
     4321 ctaaaagaat atctgatgaa gcaaaagaaa aagctaaggg ctatatcgta aacaatttag
     4381 gagaaaacta ctataaaaac actggtgata aaaaagaaaa aaaggatact aaaaaggttc
     4441 aagatgccca tgaggcaata agaccaacct cagttgatag aactcctgag agtataaaag
     4501 cctcattgag caaagaccag tacaagttat acaatttaat ttggagaaga tttgtagcta
     4561 gtcaaatgga agattcagtg tttgatatac taaatgtaga atgtaaaata ggcgatattg
     4621 tatttaaggc aactggttca aaaatgaaat ttgatggtta taccaaggta tataattttt
     4681 cagaaagaga agacaaaatt ttaccaagta tagaaaaagg agatatatta aatatagaaa
     4741 atatattgcc agaccaacat tttacacaac caccagcaag atatacagaa gctagtttag
     4801 taaaaacact agaagaacta ggtataggta gaccaagtac gtatgctcca acaatagcta
     4861 ctatactgaa tagagagtat gttgaaaagc aaggaacaag tctatgtcca acagaacttg
     4921 gaataatagt tactgatatt ttggaaaata atttccataa atttatagat gtagatttta
     4981 ctgctgatat ggaaagtaaa cttgatgtta tagaagaagg caatacagat tggaaaaaga
     5041 ttgtatcaga atcatatgca ccacttaaag aggctattga agaagctctt gaaaatatag
     5101 aaaaagtaaa tatggatgaa gaaactgatg agatttgcga gaactgtggg tctaatatgg
     5161 ttatcaaata tggtagattt ggaaagttta tggcttgtaa aaactatccc gattgtaaaa
     5221 caactagacc aattatgaat aaaattggtg ttaaatgtcc aaaatgtgaa gaaggagata
     5281 ttatacttag aaaatctaaa agaggtaaag ctttttatgg gtgttctaat tatccaaact
     5341 gtgactttgt agcttggaat aagccaacag gagagttttg tgagaagtgt ggttcatata
     5401 tggttgaaaa agtgactaaa tctgaaacaa aaacagtgtg ttctaataaa gaatgtaata
     5461 aagagacaaa agagcataat gacggaaaag agtgaaaatt gaaaaaaatc acataattct
     5521 cttgaaaaag gttccaatct atgataatct ttcaagtgta aacaactaat agaaaaataa
     5581 attttcatta aatgtgaaaa taatattaga aaatataaaa ggttattaaa aggcctaaat
     5641 ttcatgagga gatgattaaa tggcaagtga agtgttacaa aaaacaagga aaataaataa
     5701 aacattacaa acaagtggtg gaagcagtgt ctcttttgat ttactggccg gagcattggg
     5761 cgacgtttta agttctaatg tttatgtagt aagtgcaaaa ggtaaagtac taggtcttca
     5821 tttaaatgat gttcaagaca gttcagttat agaagatgag tatactaagc aaaagaaatt
     5881 ttcagatgaa tatactcaaa atgtgttaaa aattgatgaa acattagaaa atttaaatgg
     5941 tgagaagata ttagaaatct ttcctgaaga acatggaaga ttacaaaaat atactacagt
     6001 agttccaata ttaggaagcg gtcaaagatt aggaacattg gtactttcaa gatattcaaa
     6061 ttcattcaat gatgatgatt tagtaatagc tgaatacagt gcaactgttg ttggtcttga
     6121 aatattaaga gcaataggtg aagaattgga agaagaaatg agaaagaaag ctgtagttca
     6181 aatggcaata ggcactctgt cctactccga gcttgaagca gttgaacata tttttgctga
     6241 attggatgga aaagaaggtc tacttgtagc aagtaagata gctgatagag ttggtataac
     6301 taggtctgta atagtaaatg cacttagaaa atttgagagt gcaggtgtga tagaatcaag
     6361 atcattaggt atgaaaggta ctcatataag aatacttaat gacaaactta cagatgaatt
     6421 aaaaaaatta aaaaacaatc aataatctat attttatagg tttagattag aaaaggtatc
     6481 tatttcaaat agataccttt tttagacgaa ttattaaggt aatgataagt atattaaaaa
     6541 ggagtgagaa aatgccttta gcagataaaa taagaccaaa aacaatgaat gatgtaatag
     6601 gtcaaagtca tataataggt aatggaaaaa tattgtctaa gatattacaa tctaattttt
     6661 taccaaatat gatatttttt ggtcctcctg gtgttggtaa aactacagta gcggaaataa
     6721 tagcagaaag aagcaataaa agcttctata aaataaatgc tactaattca tctttagaag
     6781 atataaaaaa ggtgatatca gagcttggaa gtataaataa tgtaaatgga gtcctattat
     6841 atatagatga aatacaaagt tttaataaaa aacagcaaca atcaatatta gaatttatgg
     6901 aaaatgggag tataactttg attgctagca ctacagaaaa tccatatcat tatgtatata
     6961 aagctctttt aagtaggtct actgtatttg aatttaaacc tttagaaaaa cttgatgtag
     7021 aaaaaggact gaaaagagct gttgaagtat taaatgaaga tagctatatg gatattgagt
     7081 gtaataataa tgctattgaa gatatagcaa ttctttctga tggagatatg agaagagcct
     7141 taaatatttt agaagtggta gtctattcaa ctaaaccaaa taaggataat ataacttata
     7201 tagattcaga tgtaataagg gcatctacat ttaataaaat tataaactat gacaaaaatg
     7261 gtgatagtca ttatgatatt ttaagtgcat ttcaaaaatc tataagagga agtgacccac
     7321 aggcatctat acactatcta gcaagactta taaaaggtgg ggatttgata agtatatgta
     7381 ggagactttt agtaatagct agtgaagata taggtttagc ttatccaaat gctattgtaa
     7441 ttgtaaaggc atgtgtagac tcagctatgc aacttggatt tccagaagcg aaaataccat
     7501 tagcagaagc tactattctt ttggctacat ctccaaagtc taattctgca tgtatagcaa
     7561 taatgaaagc tatggatgaa ctggataaag agtttgttaa ggatatacca aatagtataa
     7621 aggattctca ctattctggt tctaaagaaa taggtagggg ttgtggatac aaatatcctc
     7681 ataattttaa aaatcattat gtaaagcaac agtatttacc agatagcata aaagatagta
     7741 tttattacat accacaagaa aataaaactg agaaaaatat aaaaaaatat ttagaatatc
     7801 taaatagtga tttttagact gaactttatg tatcgggggt agatattatg attataaaac
     7861 ttaactcaac ttatcacagt agagttatga aatacttaaa aaaagaacct gagtacaatt
     7921 tgtttataat aggggatatt gaaagatatg gatatggaaa taatttttta aatatatggg
     7981 cagatgtggg tgaacatggt gaaataaagg caatattact taaatatttt gaatttatga
     8041 tattttattc tgatggagag tatgatgttg aaggttttta taatctactt cgtaatacta
     8101 attacgaaga aatatctggg aaaatatgtg ctgtagatgc tttagcaaag agattaggac
     8161 ttaataattt aaaggtagtt gatttttgca aacttcaaac taaaaagttt ttgatagata
     8221 ataattgtaa tgcaaaggtt aaaagaatta ggcttggaaa tctaaaaaag acagtgaaac
     8281 tttatgactt aatagatgaa tttcacagta caactttaga gaatttaaaa aatggactaa
     8341 ggacaggtag aggatattgt atagagatta acaaacaggt ggtgtcaatg gcaaagagta
     8401 cttcagaaaa taggactcat gcaatgataa ttggagtagg aacacatcct aaatatagag
     8461 ctaaaggact tgctacaaaa tgcctaatta aactatgttc agagttactg agagaaaaca
     8521 aaattccttg tttgttttat gataatgaag aggctggaaa gatatataaa aaactaggat
     8581 ttgaaaacat tggaaaatgg ggaatttatt caaaataaaa aattaaaatt aatcttgaca
     8641 aaattagtag ggtataataa aattatcttg aaagtaattc ctactaaaac agtatgaatt
     8701 agatttctat atagaggtga taatatatga agttgtctac taaaggtaga tatggactaa
     8761 aagcaatgtt tgaattagca ttaaatcaag acaatggacc agtatcatta aaatttattg
     8821 ctaagaagca aaaaatatca gatcagtact tagaacaaat attttcatct ttaaaaaaat
     8881 cgggtctagt aaaaagtgtg agaggtgctc aaggaggtta tttgctttca aagaatgctg
     8941 aagacataac agtaggagat atacttgtag tattagaagg accagttgca ttatctgact
     9001 gtgtactaga tgaagatgta tgtgaaaact caaacatgtg tgtaacaaaa atagtttggg
     9061 aaaagatgaa aaagggtata gaagatgtta tagattcaat tactcttaaa gatatgataa
     9121 atgactataa caaaaataaa ttagaaaatg atataacaaa tattaaaaaa tagatttgga
     9181 gatgaatgta atggaaaaaa gaagattgta tatggattat tctgcaacaa cacctattaa
     9241 aaaagaagta ttggatgcaa tgatgccata cctaacagat tattttggta atgcatctag
     9301 ttttcacaca ttcggaagag aagcaaaaga tgctttggat aaagcaagag aacaagtagc
     9361 agcccttata aatgctgaac ctagtgaaat atattttaca gcaggtggtt cagaaagtga
     9421 taactggaca ctagagggtg tagcttatgc taataaaaac aaaggtaatc atataataac
     9481 ttcaaaaata gagcaccatg caatacttca tacatgtgaa tacttagcaa aacatcatgg
     9541 atttgaaata acttatttag atgttgatag cgaaggaaaa gtagacttaa agcaactaga
     9601 agattcaata aaagatacta ctatacttat aagcataatg tttgcaaata atgaaatagg
     9661 aactatacag cctattaaag aaatatccga aatagctaaa aaacataaga tattattcca
     9721 cactgatgca gtacaagcta caggtaatat tccagtagat gttaaagaat taggaataga
     9781 tttgatgagt atgtcttcac ataaaatata tggaccaaaa ggtgtaggag ctttatacat
     9841 aagaaaaggt gtaagacttc ataactttgt ccatggtgga gcacaagaaa aaagcaaaag
     9901 agctggtaca gaaaatatac ctgcaatagt tggatatgga aaagcagcag aattagctaa
     9961 ggaaaatatg caaaatcatg tagaaacttt aactcgtctt agaaataagt taatagatgg
    10021 tgtgttagaa agaatacctt acacaagagt aaatggtagc ttagataata gattgccagg
    10081 aaatgcaaac tttgcattcc aatttataga aggtgaagga atacttttat tattagatat
    10141 gttaggaatt gctggttcaa gtggttctgc atgtacttct ggttcattag acccttcaca
    10201 tgtacttctt gcaataggtt taccacatga aatagcacat ggttcattaa gacttacagt
    10261 tggagatttt acaacagatg atgatataga ttatatacta gagaacttac caaaagtaat
    10321 agaaagactt agaagcatgt caccacttta tgatgatgcg aagaaacaag gtttagtaaa
    10381 atagacataa agataaagca taaaaaatag acataaattg agtaggagga aaagataatg
    10441 caatatagtg ataaagttat ggaacatttt atgaatccaa gaaatatggg agaaattgat
    10501 aatgcaagtg gtgtaggtga agttggaaac cctacatgtg gagatataat gaagatattt
    10561 ttagacatag atggagatgt aataaaggat gttaagttta aaacatttgg ctgtggttca
    10621 gctatagcaa gttcttctat ggcaactgaa atgataaaag gaaaaactat aaaagatgca
    10681 ttagaactta caaataaagc tgttgcagaa gctttggatg gtttaccacc agtaaagatg
    10741 cattgttcag ttttagctga acaagctgta aaagcagccc ttatagatta tgctcaaaag
    10801 aacaatatcc atataccaga attggatgga tatgttatag atgatgctca tgaccatgat
    10861 gttgaagaag aagaataaaa ataagtgatt gtgttatgag aagtttacaa tgattaaaaa
    10921 gaagtactaa tgatataatt aggacataaa aaatagctgt ttttaaaatg gctatttttt
    10981 attgcctaaa ttaagtattt taacttgatt tagaggttaa ttagttaaca aaataatcca
    11041 taagattcaa ataataaaga tgattatttt tttagaaaat agaacaagct atattttaaa
    11101 taagtacaat acttgctcta tatggtataa taataagtaa tgcaaaagat gaggtgaaat
    11161 ttgtttatga ataaaaaagt aatgataggt atgagtggag gagttgatag ctctgtagct
    11221 gcatatcttt taaaacaaca aggatatgat gtcattggtg ttactatgaa gttgtggcaa
    11281 gatgatgatg ttgttgaaat tgaaggtggt tgttgctctt tatctgctgt tgaagatgct
    11341 agaagggtag caaataaaat aggtatacca ttttatgttt taaattttag agaagtgttt
    11401 aaagaaaaag taatagatta ttttatagat gaatatttgg aaggaaaaac tcctaatccc
    11461 tgtatagctt gtaataaaca tataaaattt gatgattttt ataagaaggc tagacaaata
    11521 ggttgtgatt atgtagctac aggtcactat gcaaagatag aaaaggatga aagtacagga
    11581 agatatttgc taaaaaaatc tgttacagat aaaaaagacc aaacttatgc actttataat
    11641 cttacacaag agcaactaga gcatacttta ttgccaatag gtgactatga aaaagataga
    11701 gtaagagaaa ttgctaagga aatgggtatg gcagtacaca ataagcctga tagtcaagaa
    11761 atttgttttg ttaaagataa tgactatgca aattatgtaa aaaaacattc taaaaagcgt
    11821 attgaagaag gtttttttgt agatactaaa ggcaatatac ttggaaaaca taagggtata
    11881 ttatattata ctataggcca aagaaaaggg cttggaatta cttttggaaa accaatgttt
    11941 gtaatagata taaatccaat aaataatacc atagttcttg gagacaatga agatttattt
    12001 aaaaaggaac ttattgctaa agatgttaac tttatatcaa tagatacgtt agaagaaccc
    12061 ttaagagttc aagccaaaat aagatattca gcaaaaccat caccagctac tattcataga
    12121 gtaggagaag atactattaa aatagttttt gatgaagctc aaagagctat tactaaaggt
    12181 caatctgtag taatgtatga tggagatatt gtagtaggtg gaggtataat cgagaaaagt
    12241 ttataaaaac atctaaaaaa ttaaaaaaat aagcaataaa gtatattaag agtaaataaa
    12301 gtttataaaa taaatttatt gtatatgaaa atatagtatt gattaatttt gtaaaattta
    12361 gtaatataat tagtatatta attaaaaaac aaatacagtg ataaggaata gtaaaatatt
    12421 tgatgtctta cagagagagg gaaatgctgg aagcccttag aatatgatat ttgaagcagc
    12481 cttttagtag caatcttgaa caatttatct tttataaaga agatttaagt agagattgac
    12541 ggtagttacg ttatagctaa ggttttccga agatatcata ctttgtatga taattagggt
    12601 ggtaccgcga ttgtcctcgc ccctataaat ttatttatag ggacgaggtt ttttatttaa
    12661 acgaaaaaat aaaatttatt aggaggaaca tgatatggaa aagatgggat taaatgaaat
    12721 aagaagtaag tttttagaat tttttgagtc aaaaggtcat tatgtagcaa atagttattc
    12781 tttagtacca aacaatgata agagtttgtt acttataaac tcaggtatgg cacctttgaa
    12841 aaactacttt tcaggtgtag aagtacctcc aagtgtgaga atgtgtacat ctcaaaaatg
    12901 tataagaaca ggagatatag aaaatgtagg tataactgca agacatgcta cattctttga
    12961 aatgatgggt aatttctcat ttggagatta ctttaaaaga gagtcaatca aatggggatg
    13021 ggaatttgtt acagaatggc taaatatacc agaagataag atatgggtga ctgtatatga
    13081 agaagatgat gattcttatg atatatgggc aaaagaaatg aatttcccag aagaaagaat
    13141 ggttagactt ggaaaagatg ataatttctg ggaaataggt acaggtcctt gtggtccttg
    13201 ttcagaaata tattttgata gaggagaaga atatggatgt gacaatccag actgtaaacc
    13261 aggctgtgat tgtgataggt atttagaatt ttggaaccat gtatttactc aatttgatag
    13321 agatgaagaa ggaaattata gtctattaga aaacaagaat atagatacag gtatgggtct
    13381 tgaaagaatg ggatgtatta tgcaaggcgt agacactatc tttgaagttg atacaattaa
    13441 atctatacta gaagcagttg agaagttaac aggagtaaaa tatggagaga atccaaaaaa
    13501 tgatatatca ataagaataa taactgacca cataagagct gtaacattct tagttagtga
    13561 tggagtgctt ccatcaaatg aaggaagagg atatgttctt agaagactcc ttagacgtgc
    13621 tgctcgtcat ggtaagttat taggagtaaa agaattattt ttacagaaac taatagatga
    13681 ggttataaaa gtaaatgata aagcatatcc agttttagtt gaaaaagaaa gttatattaa
    13741 aaaagtagtt ggaatagaag aagaaaaatt caatgaaact atagaccaag gaacagaaat
    13801 attgaactct tatatagaag tattaaaaaa tgaaggaaag actgtcctaa gtggtcaaga
    13861 agcttttaaa ctatatgata cttatggctt ccctatagac ttaactaaag aaatattaga
    13921 agaagaacat ctatctgtag atgaagaagc ttttaatgaa gaaatggaaa agcaaaaaga
    13981 aagagctaga aatgctagag gaaatatgga tggagaaagt tggaaggaag accctctatc
    14041 aaaattagaa tctacagttg acagtacatt taatgggtat agtgaaattt atggtgaagg
    14101 aactattgaa gctatagtta aagatgatga gctagttcaa agtgcagaag aaggcgataa
    14161 ggtatctata gtactagata acactacttt ctatcctgaa ggtggaggac aagttggaga
    14221 ttgtggattg ataactaatg aaaacttagt tttagaagta ttaaatacta aaaaaggtgc
    14281 taataatagt ataaaacata taggtataat aaaatcagga agaataagta atggggacaa
    14341 agtaaagaca ctcgtagata gagaaactag aatgtcagct gcaagaaatc acagtgctac
    14401 acatttactt cataaggcat taagagaagt attaggagaa catgttaatc aagctggttc
    14461 tttagtaact ccagaaagac ttagatttga tattactcac tttgaagcaa tatcaaatga
    14521 agaattaaaa gtaatagaag aaaaagtaaa taatgttatt ttatcatctt tagacataaa
    14581 atgtgatatt atgaatataa aggaagctaa agaaaaagga gctacagcac tatttggtga
    14641 aaaatatggt gatgaggtaa gagtagtttc tatgggagat tattctacag aactttgtgg
    14701 aggaactcac ttgacaaata cttctcaagt aggaatgttc aaaatattat ctgaaggtgg
    14761 agtagcagca ggtgtaagaa gaatagaagc aataacagga aaagctgttt atgaatacct
    14821 aaaagaaaga gatggaatta tctctgaagt ttgtgtaaac ttaaaatcta aagaagataa
    14881 tttaattcaa agaataagtt ctttattaga agaaaataaa aacttatcta aagaattgca
    14941 tgatatgaaa gctaagatga gtttacaatc agttgattct atttttgatt ctaaagttga
    15001 agtaaatgga gttaatttga taactaataa atttgaaggt atggatatgg atacactaag
    15061 agaaacagca gataatctaa gagataagct tggttctggt gtagtagtac ttgcaaatgt
    15121 agtagatgat aaagttaact ttgtagttac tgccactaaa gatgtattag ataagggaat
    15181 acattcaggt aatattgtta gagaagttgc aaaaatagct ggaggaaaag gtggaggaag
    15241 acctaatatg gcacaagcag gagctagtga tgtatctaag gttgaccaag ctttaagcta
    15301 tgcaagtgag gttattaaga cacaagttaa ataacattca ctcaaggagg tttttagttt
    15361 ggaaaaagat ttagactaca ctatgaaatt tgaaggaata ccagaagata ggatgagtgt
    15421 aggagatact attgattttg tatataaagc attagttgaa aaaggatata accctataaa
    15481 tcaaattata gggtatctcc tttctggtga ttctagctat ataactagtc ataaaaatgc
    15541 aagagctata attaaaaaat ttgaaagaga tgagatactt gaagaagtaa tcacgcacta
    15601 tctgaataga aagtaggtca aataaatgag aaaaaagatt atgtcgttgt ttgcaatagt
    15661 cattatgatt tttgtatcaa ctgctagcta tggttttgct gaagaaaaag gcaaggtcat
    15721 atttattgat atgaatagga ccaatcttgg aagtatgatg aatattccaa tcttgaaaaa
    15781 tgagatagaa aagagaggct atgtagctct catgaatata agaggggatc aaggtactga
    15841 tgatagaagg tcttgtgcaa gtatgggtgc aggaacaaga gctagtgtaa ctactgaaga
    15901 ctatatcaat tttcaaaaag tagaccaaga tactgctaaa gcttttgaag tcgcaacagg
    15961 tcaaaaagct aaagaaataa atgatttagg cattaataga tctataaatg aaaatcttga
    16021 gtatggagaa tatggagcaa atttagggtc attaggaaaa actttatcag aaaatggttt
    16081 aaagacatct gtaattggaa atgctgatat aattgaaaat aatcaattag ttaaaaacag
    16141 aaacttatgt ttagtagcta tggaccaata tggaaggatt gattcaggta atattgataa
    16201 tattaataag aaagattcta aaatgccatt tggaatatct acagattatg ataaattact
    16261 agatgaaact aaaaaatcct ataaaaatag tgatgctata tttgtagaac ttggagatac
    16321 atatagatta gatatgtata gattaaatct aaatgataat acttatgata caatgaagaa
    16381 aaatattgta aaaaatataa ataaatatct tgaacaagta ttttctatgg ttggagaaaa
    16441 tgacactgtt tatatagcaa gtgcatttcc aagtgaccta gattataaga ataagagaag
    16501 actttctcca ataataaaat ttagtggaga agggaaaggt ctactggaat ctgccacaac
    16561 aagaaaggct ggaattgttg ccaatttaga tgttggagta gatatactag gtacatttgg
    16621 tcttgaaaat aaagagatgg taggtagaag ttatacttta gtaaataaag aggacaatat
    16681 tagctatcta aaatcacttt atgaaaggat agtgtctata tcttcaataa ggtcaactat
    16741 tataaatggc tttgtaggcg taatatctgc ttcttgggta attgccatgt ttgcgatttt
    16801 ctttagaaat tatatgccac ataaagagcg tgtattcaaa gtacttaagg agtttataaa
    16861 attaggcata ataatgccac tatcatttat gttagctcct ttgatgaatt ttaaaactcc
    16921 aatttcaata tctttgggaa tatttgcaac tacgctaata ttatacttgt taggtagaaa
    16981 attatttaag gacaatgata taaagcacat gggatttttt gcaacactta ctattttagt
    17041 catagttata gattctgtat ttggaacata cttaatgcaa aataacataa tgagttatga
    17101 tgctgtcata ggagctaggt actatggtgt aggaaatgaa tatgaaggtg taactatagc
    17161 aagtgcagta tttggcttag cagtattatt acattataag aaggtatcta aattacttgt
    17221 tgtaattttt tcattaataa tacttataac tagtgcatat ccttcaatgg gtgcaaatgt
    17281 tggtggagct atatcagaat gtgtagcata tttgttgttt ataatgctta tatatgacat
    17341 taaaatagat tttaagaaag ctgtattatt agctgtttca gctgtagttg tcgtttttgc
    17401 atttgcagct ttagatatac tttcaggtag tgaatctcat ttaggtatgt ttgtacagag
    17461 aattttccta aatggaccag gagagatatt acaaacattt ggaagaaaaa ttcaaatgaa
    17521 tatgcaacta gcacagacaa gtgtatgggt aaatatttta cttgtaggaa ttggagtaat
    17581 tgcagtgctt atatttaaac catctagaca ctttagaaag ataatgaata attttccaat
    17641 cttgtttaaa gggtttattg catctatggt tggttgtata attactcttt tagttaatga
    17701 ttctggaata gttgctgcat ctacagcatc tatatatata cttataccac taataataat
    17761 aagtataaat atgataatat ttgaaaaaaa taactaaaaa aatttttact gttcatatag
    17821 gatttaaata aaaaatgcaa tatgattatt aatgtaaact ggatgcttac ataaaaaatc
    17881 atattgcaat ttttatgatt gataatgtaa aattctatga agcagtaagc ctgtgacctt
    17941 agttatgaga ggttcagaat atgtatttaa tctatagaat ataaatagat aaaggaggaa
    18001 ctatgttaga tggaagaata atgggtcttg atgttggtga taaaactatt ggagttgctg
    18061 tgagtgattt gatgggatta actgcacaag gtgtaaaaac aataaaaaga gttggcaaga
    18121 aaaaagatat tgaagagcta aaagcaataa taaaagaaaa acaagtaaat aaaattgtat
    18181 caggattacc aaaaaatatg aatggaactt taggtccaca aggagaaaaa gtaatcaagt
    18241 tttgtgaatt ggtaaaagca gaaacaggta ttgatgtaga gttttgggat gagaggcttt
    18301 ctacagtagc ggcagagagg tctttattag aagcagatgt tagtagacaa aaaagaaaaa
    18361 aggttataga tatgttggca gcagtaataa ttttgcaagg atatttagat tttaaaataa
    18421 attcataaaa gaactataaa attgaatact tatgatatat aatataaata ataaaaataa
    18481 aaataaattt gaggtgaatg acattatgga agaaaatata ataaatttaa tagacgaaaa
    18541 tggcgttgaa agccaatttg aaataatatt aacattagaa gcagaaggaa aagagtatgc
    18601 aatattaatg cctttagatg atgaagaagc tgaagaagct ttaatcttta gaattgatga
    18661 ggatgaagaa ggtgaaatct taataccttt ggaaagtgat gaagagtatg aaactgttgt
    18721 agctgtgtat actgctataa tggaagaaga aggtttaaat tacgacgaag atgaatcaaa
    18781 tggacttaac tagataagta attaagtttg tatataaaaa tcagatattt attatatttg
    18841 ctttaattaa accttattaa ttgtaaaagc atttatagta tatatctgtt tttttgtttt
    18901 ttattaaaaa taccaaaaaa tttatgaatt ttggtgaata atatatcttt tatgggtata
    18961 tatttatatt atgagaataa attaaattta tttaaatgtc ttgacaaaaa atgaattatc
    19021 aattacaata ttatttaata aagattatca attgaaaata gtaagggtga tgttatggca
    19081 aatacaatgg atttattaaa agacaagtta aaagaaactg gttttaagat tacgccacaa
    19141 agaagagcaa tagttgaaat attgcttaaa catgaccatt cacatctaag tagtgaggaa
    19201 atctatgatt tagttagggt tgactgtcct gaaataggct tagctactgt atatagaaca
    19261 atgcagctat tggatgaaat aggattaata tcaaaactaa acttagacga tggatgtata
    19321 agatatgaga ttagtctaca taaagaagac tgtcataatc atcatcatct tatttgtaaa
    19381 aattgtggaa agataatgga agctaaagaa gaccttttag acaatataga aaaagagata
    19441 caatcattgt acaaatttaa aatacttgat catgatgtta aattttatgg actatgtgac
    19501 gagtgtaatg gagtaagcga ttcagaagaa taaacttcta ttgcttaatg tcgatggaag
    19561 aatagtatag aacttttata ttctatgcta ttcttccatt ttttattata aaccatctat
    19621 aaaatgcaaa tatacagttt tcaaagtgat tatagtttta atattgaaaa tataattaat
    19681 cctataagta caaaaatgaa tgatttttct atgaaattac taaatggtcc tccaacagtt
    19741 aaaaacccta aagaaatttt cttttcgctt aggggataaa aaattggtat tccttgcttt
    19801 gtaaataaat ctcctaaaaa catatggctt gcataaccaa tcgtaccata aaaagataaa
    19861 ttattaatat tagtgattaa ttctatctgt tttagtaaaa aatatactat tatagttgca
    19921 aatatgctat gagaaaaact tctgtgcttt aaccagggga aaattgacaa cattagacaa
    19981 aaaattgtaa aatatatttc aacttctatg atgtataagc atatagctaa tagtataaat
    20041 attaatgata gaaaaacttt tcttagaaat gtatgggtta gttttgcttc aataattata
    20101 attgcaacaa atgtaattat tgcgcttaaa taaaaattat catttatatt tattgaaata
    20161 aagaaaataa taatattaac agcatatata aaaattctat atatgtattt ggatacattg
    20221 ttttttagaa tgaaatttga gaccatagaa ttagatttat ccaaatcggg tagtacagca
    20281 aaagtagctg aaactaaaat atcaactaaa gatataggaa ttttaaatat taaagatgtc
    20341 tggatagctg ttaagattcc tattgcacaa tgagttttac cacgcataaa tcctcctaaa
    20401 ttatccgata ttaaatcata taatatttag aggatatcaa aataaaatag gtttgtcaaa
    20461 ataatacgtt aaatgtattt taaatatagc ttaaaatatg atacgaatta tggtacaata
    20521 agaataactg gtagcgagtg cgtgatttga tattatttat gattgtagaa cgtgttaaaa
    20581 aattaaatag acagaaggag atgatgcaat tgttcaaaaa gaacaccaat aagataaagg
    20641 taatggcctt gggtggactt aacgaagttg gaaaaaatat gactgttgtt gagtataaag
    20701 atgaaataat tgttatagat gctggactta gttttccaga ggatgaaatg ttaggagtag
    20761 atatagttat accagatata acttatttag taaaaaatag ggataaaata aagggtatat
    20821 ttattacaca tggacatgaa gaccatatag gagctcttcc atatatatta aagaaaataa
    20881 atgtacctgt atatggggca agacttagta taggtcttat acaagtaaaa cttaaagaac
    20941 ataagatgaa taatgttaag ttaaatgtta taggtcctag acaagttata aagcttgata
    21001 atatggaagt agagtttttg aaaaataacc atagtatacc agatgcttac tctattgcaa
    21061 tacacacaga ccaaggtata atataccaca caggagattt taagattgac ttgactccta
    21121 ttgatggaga tgttatggat atgcatagaa tctgtgagtt gagcaaaaaa ggagttcttt
    21181 taatgctggc tgatagtact aatgcagaaa aaccaggatt tactatgtct gaaaagactg
    21241 ttggggtagg tcttgacgaa ttatttgcaa aaggaaatgg cagaagaatt atagttgcta
    21301 cctttgcatc caacatacac agattacaac aaataataaa tactgctgag aagtttaata
    21361 gaaaagttgc tatatcaggt cgctcaatgg tcaatgtagt tggagttgca aaagaattag
    21421 gatatctaga tatttctgat gacatgctta tagatttaaa tgatatatgt aagtatgaag
    21481 atagtgaatt agtgataata acaactggtt cacaaggtga acctatgtca gctcttgcta
    21541 gaatggcttt ttcagagcac aaaaaagtag aaataaagag tggcgattta gttataatat
    21601 ctgctcatcc aataccagga aatgaaaaat taatatcaag agtaatcaat ttcttatttg
    21661 aaaaaggagc agaagtagta tacagtgata tagctgatat acacgtttct ggacatgcct
    21721 gtcaagaaga attaaaactt atacatgcat tagttagacc aaagtttttt atgccagctc
    21781 atggtgaata tagaatgtta aaaagacatg ctgaaatagc tgaacagctt ggtatggata
    21841 aagaaaatat ttttgtcatg caaacaggag atgttttaga attagataaa aactctgcta
    21901 aggtagcaaa tcgtatacaa acaggaaaca tattagttga tggtctagga gtaggcgatg
    21961 taggtaatat agttttaaga gatagaaagc atttatcaga agatggtctt atgatagttg
    22021 tagttactat ttctaaggat gagggtaaag ttttagcagg tccagatata atttccagag
    22081 gattcgtata tgtaagagaa tcagaagatt taatggatgg agcaaaggat ataattaaga
    22141 atgttctaaa tgaatgtgaa gagaaaaata ttaaagaatg ggcttattta aagaataata
    22201 ttaaagaaaa cctaaaagaa tatttatatc aaaaaactaa acgaaatcca atgatacttc
    22261 ctatcataat ggaagtataa tataaaaaaa ctataaattt gaaaattaga cctcttgtaa
    22321 tttttaaagt aatatcaaaa atccaattga tattatctta agaattacaa gaggtctttt
    22381 taatacttga tttaaaaaaa tatatatatt acataaaata ccaattataa caatctttta
    22441 ttagtaaaaa ctacttatat ataaatttta ctgataggag gattcttatg gatgatgttt
    22501 caagacaaaa tgctataaaa gctttaaaac aaacaaaaat ggaaatagca ggagagtatg
    22561 gaatgaatta tgaagatgct tttgaaataa tagaaaatgc gtctaataaa ggtgttttag
    22621 aaggttactt taaaaagctt gaaaagaaaa aaaatttagg tcaaggtata tctaggcatt
    22681 tagaataaaa aaagttaaat gtgagaaaat aagtttaaat aaactttaag aggtgacatc
    22741 gatgaaaaaa ttagctagta ctgctttggc tatattaatt gcgcttactc ccttaagctt
    22801 ttcttttgca aacaataagg aaaatgctga tgccaaccaa ctaaatatat cttcaaaatc
    22861 agcaattttg atggatgttg gtagtggaca aattttatat gaaaaggatg ctcataaaaa
    22921 gcttcctcct gctagtgtaa ctaaggtaat gactatgcta cttatagtag aagctttaga
    22981 ttcaggcaag attaaacttg atgatgaagt tcaagtaagt gaaactgcat ctagtatggg
    23041 tggtagtcaa atattcctag agccaggaga aactcaaaag gttgatacat tgttaaaagg
    23101 tatagctgtt gcttctgcaa atgatgcttg tgttgctatg gctgaacact tagctggaag
    23161 tgttgaaggg tttgttgata gaatgaatgc taaagcaaaa gagttaaata tgaatgatac
    23221 tcattttgca aatacaaatg gacttccagt tgcaaaccac tatacatctg ctcatgatat
    23281 agctttaatg tctagagaac tattaaaaca cgaaatgata agtaaatact taactacatg
    23341 gatggataag gttgtagtag ggaagaaaca agttacagta ggtcttgcaa atactaacaa
    23401 acttataaag cattatcaag gagcaactgg tgttaaaact ggctttactc aagaagcgaa
    23461 atactgttta tcagcatctg caaagagagg taatactcat ctagttgctg caactttagg
    23521 agcagagact tctccagaaa gatttaatga tgcctctagc cttttaacct atggatttgc
    23581 taattatgaa agtgttaagt tatgtagtaa aggtgataat atagcaacac ttacattgga
    23641 taaggctgat gaaaacaaag ttaaattggt tgctaaagaa gatttaaatg ctcttattaa
    23701 aaaaggaagt tctaaagaat ttgagaagaa aattgaaata gtaaaaaatc ctaagatgcc
    23761 aattaaaaaa ggaactgttt taggaaagat aaagatatgt aaagataaaa aagtaatagg
    23821 cgaagtggaa ttaataaata ctaaagatat aaataaggca agctatctac aaatgcttca
    23881 aagaataatt gataatatga tataaataat tatcagatga aatataatta taatacacaa
    23941 agatatgatt ataatacaca taaaaaatac ccttatgtaa ttatttgcca taggggtatt
    24001 tttatttact atatagaagt attttataat cttatgattt ttatagaaat atagaaaagt
    24061 ttatgtatct taaaatatca tttgatttaa tatcatatat agggttgttt aacaattatc
    24121 taagtaaagg catactacca gtaagtttat tcactttctt tttattccca taaattgcta
    24181 tcccaaaata ctgtaactca tcttctggaa cactagcaat cttttgtgta aattcttcat
    24241 aaagattgca actttgtgct acatctgaaa aatctacaac agttaaatcg ttaaattcca
    24301 aagtatataa ttttttacgt aaatctttta ttatttcttt gtttcctttt aaaataggta
    24361 caggaatgtc aataattcct aagtggctat tacctgtttt gtcaattaca tctggcccaa
    24421 cagtttctgg ggcatgtttt cctagtgtaa ttcccattat agctgctgta ttggatataa
    24481 ttcccattgg taaattttcg tctattacca tgacacattt tgaattttta ttttccattt
    24541 ttatttctcc tttttacctt atttaaagtt gtttgaatca tcatttataa atatattttg
    24601 atactgcttt ggtgtaagac caataaaatt cttaaaaaag tttgtaaagt gactttggtc
    24661 tacaaatcct gtttgtatcg cagcatctat aggttctacg cctttttcta aaagcttttt
    24721 agctttgttt acccgtatat tttctaggta tctatatggt gtaatacctt ttaatttcgt
    24781 aaaattacga agtagagagt acttattcat acctgctata gtgcttaatt cgtcaagtat
    24841 tatatgttca gcataattat tttctagata atcacatact gcttgaattt ctatatttgt
    24901 attttccaag ttagattgca aatttggctc tgtatgttct tcaattaatt gttcaattag
    24961 gaagaaaaaa agctcttcct ttttaaaatc caattcttct tccatgatta tatagtgtaa
    25021 ttcttgtagt agaggtacta actcactgcg aaatataaca ggttgattaa attttggtaa
    25081 ataattttta cctgtaattt caaagacggt ttttttcatt atttctggct taatattaat
    25141 acatctgtaa tcaagtactt tatcatctat ttgttcacaa gtatgactat caaaagggtt
    25201 aaaaagtagc aaatctccag tacttgtggt atactcttta tttttacaag ataaatatct
    25261 ttgacctttt tctataaaac caattacata atgttcatga aaatggtttg ggaatttttg
    25321 cattatgcct ttaaatctat atgcttcaat ttttaattcc ctatcaaagc atactgtgcg
    25381 ttcttcttgt ctcacttagg taacctcctt tcatgttata caaagtgtaa cacatatttt
    25441 tttatacttc ttgtataata ttgactaaga gacaataatg tcaattcatg tcaatataat
    25501 attacactta cagtaggtgt aattattata ttttcacaat gaatatgata taataataaa
    25561 agttataaaa taaaaagcaa attttaattt tatgcctaga agttaaaatt attataatgt
    25621 aaaataattt tgattataag ctatttaatt gttataataa tattaaaaat ttaatttagg
    25681 agatgtacat taatgttaag tatgaatata ggagaaatag tagcaagttt agttggtata
    25741 gctatggcaa tatcaataca tgagtttggc catgcttatt cagctcactt attgggagat
    25801 gatacagcta aagcttatgg tagaatgaca ttaaatcctg taagacatgt tgacccaata
    25861 ggtcttttgg ctatgtttat ccttaagata gggtgggcaa agccagtacc agtaaatcca
    25921 aataacttta aaaactataa gataggaaac ctgatagttt cacttgcagg cgttttttgt
    25981 aatgttttga ctgctatact gtgtgtaatt atcaataagt atgtgcatat gtatgctata
    26041 aatacaatag ctgaatatgt gtttttatat agtattggat ttgcagcttt taacttactt
    26101 ccgatacctc cacttgatgg atggggagtt atatctacat ttgtaccata taaatggaat
    26161 gaatatttgt ataaatttga aagtatgagt tatataattt tattaattgc tttatttact
    26221 ggtatgtata gaataatatt aacaccaata tatgcagtgt tgctaaaaat agtatattta
    26281 tttgtttaat ataataaaca gattaaagag ggattgatat gaagtataat atacaattac
    26341 aagtctatga gggaccatta gatttacttt atgatttaat aacaaagcat aaaatagata
    26401 taaaggatat atccataata gatataacta agcagtatct aaattatttg aaaatgctag
    26461 ataaaatgga tttagagata actagtgaat ttataactat ggcatcaaaa cttttagaga
    26521 taaagtctaa atatctgctt tataagcaaa aagatgaaga agaagaccca agaatagaac
    26581 ttatggaaaa gttagaagaa tatagaaagt ttaaggtagc atcacaagat ataaaagaaa
    26641 atataactta tgttaatgag agattttata gaaataaaga agagataatt attgatgaca
    26701 atgtggattt agaagatata tcaattgaag caataaaaaa tatacttcca tacatattta
    26761 aagttaaaac atcacagata gaaaatgcta atgatgagaa actagataaa atagtaagga
    26821 agaaaataat atctgtagaa gaaaaaatat tatatataag agatattata aaagagaaga
    26881 ttgaagttac ttttacaaat attataaaat catatgaaaa tgatgaaata atagctactt
    26941 ttttatctat attagagctt ataaaagaaa aagaaatagt cgtagtacaa gatatatttt
    27001 ttgatgatat tctaataaga aaaagttcgg agtgttaaaa tgaaaagaga agatataaag
    27061 tatattatag aatctgttat gtttgcatat ggtgagccaa taagtataaa agaattaaac
    27121 tatataataa ataaagaact ttcatcaaaa gaaatagaga ttatgttaaa tttacttatt
    27181 gaggagtaca gagaacaaaa tagaggtatt caaataatca agttagagaa taagtaccaa
    27241 atgtgtacaa ataaagacta tgcagaatat ataaagaaga ttattgaacc aaagaaaaag
    27301 aaaagtttga gccaagctac attagaaact cttactataa ttgcctacaa acaaccaata
    27361 acaaaggtag aaatagagga tataagagga gtaaaatgtg ataaggtatt acaaactttg
    27421 tttgaaaatg aacttataag agaagctggt agattgaata agatagggaa accaataatt
    27481 tataaaacaa cagatgaatt tttaaagttg ttgaatatag aaagtcttga agaattgcca
    27541 cctatagaaa attatcaaga agtggcaaca aatgaataaa aatataaaat cacaataatt
    27601 tagtatgtaa ttttaaattt taaaagattt aaaatgctct aagttgttgt gtttttttgt
    27661 ataaatataa aattataatt ctcttaa
//
LOCUS       DAMJWS010000054        27275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMJWS010000054 DAMJWS010000000
VERSION     DAMJWS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.054"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..896)
                     /locus_tag="PC399_002351"
     CDS             complement(<1..896)
                     /locus_tag="PC399_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2593208.1"
                     /translation="MKLKHIAIIGSLFPILFSIVLFFGVLMSADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVNVLLAIIQVESGGTAKDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQMQYDATQHIPLSQA
                     KAGELVFFHSTYNAGSYVTHVG"
     gene            complement(893..3085)
                     /locus_tag="PC399_002352"
     CDS             complement(893..3085)
                     /locus_tag="PC399_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2593209.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPENEDGKTREEVVKTEIESNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVAGAVAGSTVSNARTTRNNSAKDNRGNTSSSMGQR
                     AGSKVGAVLDTKNKVKDKANAMKETVKDIPTQTAYAVHSAKEKAKTSVSDFKRGMVQE
                     QQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQARKTDGSATTGATRPHERPVTASA
                     IPKTNVEKTQEVKRPATTSTSKAGEPVKTNSVKERPLSSGTSDGKIPQSAQTVHSKNV
                     EKVVSHETHQNYKAERTTKAQTVEQAKRTTEHTEKNRNLVTKKGQKKK"
     gene            complement(3085..5535)
                     /locus_tag="PC399_002353"
     CDS             complement(3085..5535)
                     /locus_tag="PC399_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2593210.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNLKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEHQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRCEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGEILSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            complement(5522..5911)
                     /locus_tag="PC399_002354"
     CDS             complement(5522..5911)
                     /locus_tag="PC399_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2593211.1"
                     /translation="MKKIRSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPAFLTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLQKEIFNETITAVRSVSYVPD"
     gene            complement(6029..6532)
                     /locus_tag="PC399_002355"
     CDS             complement(6029..6532)
                     /locus_tag="PC399_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2593212.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIDELNEMYEMIEELPDYIVDCLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            complement(6535..7038)
                     /locus_tag="PC399_002356"
     CDS             complement(6535..7038)
                     /locus_tag="PC399_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2593213.1"
                     /translation="MQETRVLVETKRTTGEETTSYWFDLPIEVAEFEEKLGVSTESQD
                     YRIIEKLLPYADEVHEHTSVYQLNELDYMYRQLSSDMQEEYTMLHTVYENLEALYICR
                     NVITLYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFETYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(6957..7253)
                     /locus_tag="PC399_002357"
     CDS             complement(6957..7253)
                     /locus_tag="PC399_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2593214.1"
                     /translation="MNDIFRDMQAKVGCENISDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSCQTLKDVMKQQKGREEQCRKQGYWWKRKEQLAKKQHHTGSTCR"
     gene            complement(7350..7952)
                     /locus_tag="PC399_002358"
     CDS             complement(7350..7952)
                     /locus_tag="PC399_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2593215.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            complement(8037..8198)
                     /locus_tag="PC399_002359"
     CDS             complement(8037..8198)
                     /locus_tag="PC399_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593216.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALVAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            complement(8365..10275)
                     /gene="ltrA"
                     /locus_tag="PC399_002360"
     CDS             complement(8365..10275)
                     /gene="ltrA"
                     /locus_tag="PC399_002360"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDF2593217.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNHILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11017..11151)
                     /locus_tag="PC399_002361"
     CDS             complement(11017..11151)
                     /locus_tag="PC399_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2593218.1"
                     /translation="MWELLKDLLLVSFGMGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            complement(11159..12358)
                     /locus_tag="PC399_002362"
     CDS             complement(11159..12358)
                     /locus_tag="PC399_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2593219.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPNNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIGYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIRKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTQPEPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHYQIIKQQTATVEE
                     MVANVKE"
     gene            complement(12560..13954)
                     /locus_tag="PC399_002363"
     CDS             complement(12560..13954)
                     /locus_tag="PC399_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2593220.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNMKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFSSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            complement(14071..14556)
                     /locus_tag="PC399_002364"
     CDS             complement(14071..14556)
                     /locus_tag="PC399_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593221.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQAFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            complement(14654..15037)
                     /locus_tag="PC399_002365"
     CDS             complement(14654..15037)
                     /locus_tag="PC399_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2593222.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITRRT
                     YDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIKAD
                     DIILKNKGGQVGNPQNHAPQGQPKK"
     gene            complement(15052..15378)
                     /locus_tag="PC399_002366"
     CDS             complement(15052..15378)
                     /locus_tag="PC399_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2593223.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFTPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            complement(15614..18658)
                     /locus_tag="PC399_002367"
     CDS             complement(15614..18658)
                     /locus_tag="PC399_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2593224.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTDNHNGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITNGNG
                     NYSIAGATYGVFSDKDCTKQIATLTTDDSGNTEVAEVKATTVYIKELSAPLGYKVDTT
                     VYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTTAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAFLPL
                     GTLTVEETKSPTGYLLDGAYMQANGSEEQIKALYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETKDTKPQGSATLKDTAFDIISLNDNAVLVEGKIYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSKWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGMELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAEIIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPMKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DGKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETPTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            19186..20193
                     /gene="asrA"
                     /locus_tag="PC399_002368"
     CDS             19186..20193
                     /gene="asrA"
                     /locus_tag="PC399_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2593225.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKDVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            20194..20985
                     /gene="asrB"
                     /locus_tag="PC399_002369"
     CDS             20194..20985
                     /gene="asrB"
                     /locus_tag="PC399_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2593226.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            20998..21969
                     /gene="asrC"
                     /locus_tag="PC399_002370"
     CDS             20998..21969
                     /gene="asrC"
                     /locus_tag="PC399_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2593227.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            22026..22802
                     /locus_tag="PC399_002371"
     CDS             22026..22802
                     /locus_tag="PC399_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2593228.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            23073..25091
                     /locus_tag="PC399_002372"
     CDS             23073..25091
                     /locus_tag="PC399_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2593229.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            25306..26163
                     /locus_tag="PC399_002373"
     CDS             25306..26163
                     /locus_tag="PC399_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2593230.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            26260..27129
                     /locus_tag="PC399_002374"
     CDS             26260..27129
                     /locus_tag="PC399_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2593231.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         8264 a         5028 c         5017 g         8966 t
ORIGIN      
        1 attcccacat gggtaacata cgaaccagcg ttataggttg aatggaaaaa gaccaactcc
       61 ccagcttttg cctgcgataa agggatatgc tgggttgcgt catattgcat ttgtgccgtt
      121 ctcggtaaag agataccagc ttttccataa caccattgcg tcagtcccga acagtcaaag
      181 gaagtgttgg gattgctacc gccatacaca tatttccagc cttgatattt caacgcttca
      241 ttcattacct tttgtgccag ttctcccgat acctgcggaa cagttaaata ctgattgact
      301 aattccacat aaaacatatt tccatagcca taccgccagc ccccattttt cgcaacggct
      361 atcgggttgg tgtaggttac ttttttaccg ccagatttct cacgggcaaa gctctctgca
      421 aggttaaaag tatgtttctt tccttttcct gccacatatc ccacatagcc accgccatag
      481 ttataggact gtatcgccac attcaaatca tttattcctt gatttttgca ggaagaaagc
      541 agggacgcaa aatatttaca cccctgcttg attgagcttt ctgtatctaa cgagttgggc
      601 ggtaaaccaa gactttctga actctgcatg acatcttttg ccgtaccgcc actttctact
      661 tgaatgatag ccagtaacac attcacatac tcagaaatac cgtattcttt agcgtacttt
      721 tccaccatag gctgatgttt caagacttct gcggataaat tcatacccgt aatgcccgaa
      781 gaaaagttgc tgttctcgtc gtcgctgtcc gcactcatca gtaccccaaa gaaaaggact
      841 atggaaaaga ggataggaaa cagactgcca atgatagcga tatgtttcaa tttcattttt
      901 tcttctgtcc tttcttcgtt acaaggttac ggttcttttc agtatgttct gttgtacgct
      961 ttgcttgttc aactgtttgt gcctttgtgg tacgttctgc tttgtaattc tggtgtgttt
     1021 catgcgatac cactttttct acatttttac tatgtactgt ctgtgccgat tgtggaattt
     1081 ttccgtcaga tgtacccgaa gataagggac gttctttcac actatttgtt ttgactggct
     1141 cgcctgcttt ggaagttgaa gttgtagcag gacgtttgac ttcctgtgtt ttctcaacat
     1201 ttgtttttgg aatggctgaa gctgtgactg gtcgctcatg gggacgggta gctcccgttg
     1261 tcgctgatcc gtccgtcttt cgtgcctgcc ttgcttcttg tgctttttga agttccatac
     1321 gcttgtcagc gatattcttc ttatgctgtt cctgcttttc caagcgtccc gactgtctgg
     1381 attgttgttc ctgcaccatg ccacgcttga agtccgatac acttgttttt gctttttcct
     1441 ttgcggaatg gacggcataa gcggtctgtg tcggtatatc cttgaccgtt tctttcatag
     1501 cgtttgcctt gtctttgacc ttattcttcg tatctaagac agcacccacc tttgaaccag
     1561 cacgttgtcc catgctggaa gatgtattcc ctcggttgtc ttttgctgaa ttattccttg
     1621 ttgtccttgc attagaaacc gtactgccag ccaccgcacc agcaacaccg ccagcggtaa
     1681 cagcaccagc aagccgtctt tccatacgcc tagccctatg tgccatataa agatacggtc
     1741 tgcggaaaat tcttcgtccc atgctttgac tgtcgttagc gttcaaagag aacatactca
     1801 ttaaatcgcc taacttcatg tagataccag cgaaacacac tatctgcaag aacgccacca
     1861 taaagaacgg gtagtctgtg gaaatgttat aaaacatact ggaaatgcta aatgccaccg
     1921 ttacaatgag cgtaattcct gcccttgtca ttatggtatt aaataccctc acgattgcct
     1981 gctttgccat gctttcatag ctcggtatca tggatagtaa aaagctgata ggtaaaaaca
     2041 tggcgaagat aataaaaagt atctggctga acaacatcat acccgtaagc aagaatacaa
     2101 atatcgtgat acctaagttg aagaacaaga ggaagaacac catacctaaa cggtttacga
     2161 cttgcggtat cgtcagattg ttgttgtcgt tactttcgat ttctgttttc acgacttcct
     2221 ctctggtttt gccgtcctcg ttctctgggc ttgccgatac aagagcttct acacggtctg
     2281 tcccgatttc ctctgcgttg ctgttcccaa actgtaagag tagccacggc tgttccactt
     2341 gaatagagaa taggctgtcc cttatcaagt ccacgctgtc cttgccctcg ctgtcagagt
     2401 ttgggagcat gatttttgtt cccaagtcca aagaagcggt actgatgtcg cttgaaaatt
     2461 catttatctt tttgatgtag tcgggagcat aggcaataaa tgaagccgac aaaataaaca
     2521 ccacaacaaa gttgataacg gcgtgaagtg ccttgctggt ttctctttta atcagtcccg
     2581 tataggcaac atatagtccc accactaaga taatgagaag caggaaaccg acatagaagc
     2641 cagaggttga aaaaccgctt gaggtaacgc ctgcaagggt ctgtatgctc tgcccgatac
     2701 tgtctgccat atcattgatg aagtccagct tgtaggcttc ctgcactaca tagcccgtgg
     2761 cattacttaa atataaactg atagtccaga caaaattagt gatacagtac aaaccatatt
     2821 gtactgattt tccaatcccg tccagccagt tccacggcaa ccaagcccag ctattatcca
     2881 catagaagtc aagctgatag ttggaaagcg gatatttgga atatagattt tctgcgttta
     2941 tcgtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa
     3001 gggagagtgc caccacgaac agagccattt tgagaaagtg gaaaatcttc ttttttgtga
     3061 acgcactctg tatcctttct ttcatcactc cacctcattt ctctgtatag gcggtctggt
     3121 atcaaaggcg tgtagcagtt cttcaaagac ggggtgtatc tgtaccacgc cgacacgccc
     3181 gtataagtcc tgcaacaagc attgtccgtt ctccaaatca cgaagtcgct tctgattgtt
     3241 ttcgtcctct ttgtcgatac cgaaaaattc taaagtctgt tttatctcgt tgatgtcagt
     3301 agaacggaaa gcgaatttta agccgatatt gtttttcaga ctttcctttg acacgtcccc
     3361 agaagattgc gtaacgaaat acacacctgc ctgcatagct cgtccagcac gcaccagctt
     3421 gttagataaa atttcgcctt gtgccacatt taagaacgcc cacgcttcat caaggtctac
     3481 gattttgaag atagagcggt cgctgtggat aaaatctaag gcaaaggtac taatcacaat
     3541 cagcatggaa accgatagta attcaatggt cgtgtattcc tcaaaagtcg tgtccttatc
     3601 tggcagtaca aggtcggcta cttgaatgat attgagctga ttatctaaac tgatagcatt
     3661 ttccaccgtt ccgtctgaaa acagaagatg tgcaaaatca tagtctgtaa agctgtcgat
     3721 atggtctgcg atattgcgtg aaattgcggt atcttcacag cgtaactcgt ctatcacatg
     3781 aagcaagccc cttgtttcac tctgggtaac agaacgcacc gccttacgaa gtacggggaa
     3841 tttttcgccg tccctagagg aaataccagt taaaaaggtt agtatgtcga ttgccagact
     3901 ttcagcgtct ttcacattct tcataatcac aaacgggtca agaagccctg cgttgtcctt
     3961 gtcgcttgtt aggtttacga tattgatttc atgggcgatt tctggaagtg tttctttcca
     4021 gtttccacgc tctgattttg ggtctaagat aaccgcttga ccgccaaaca aaacagaata
     4081 gtacacaagc aggttattac agaatgactt cccaccgcca agcgaaccaa caaaagcaga
     4141 agcaagggca tttgtaactg taccttttac gccttgactg gcaagggacg gttgcagata
     4201 tacatttctt cccgtatcaa cggaatagcc catatagata cccgtgtttt cccctaactg
     4261 ctgtgtcgca ccaaaaccaa gcccagctaa gaagtctgat tttacatact gcacataatc
     4321 attgatatat cgcttgctgg ctggtagaaa ttcagaatga agccccagca tatccccagc
     4381 aggacgtact aacttcacat tcaaatcgtc ataaaaatct ttgacttcat cacaacggcg
     4441 tttcagttcg tccaaatcat cagcagacac acggataaca taagagagct tatacatact
     4501 ttcttttgtc tggtctaaat cggtttccag ctcgtccacg ctgtctaaag cgtctactac
     4561 atttgagctg gtttcacttc cagcttgata agcgtgatta tcaaggtctt tcagttcctt
     4621 tttcttgttg cggacggtcg ttaatgcttt tcggttttcc acgatttcta cattcatgct
     4681 cgtatcaacg ggaaaggtaa attgctgttg ctggaaatag aagatttcac tagacggaaa
     4741 atcaagctcg cctacaatcg cattgacggt aaaataggac acataacttt ccttgtcctc
     4801 atgttccaat cgtaaatatc gctggctttc ctcaatcaca caccttgtcg ggcggataag
     4861 gtcgtaatac ttgataagtg tttctttctt atactttttc tttggtagct ggtgctcata
     4921 atcttcatag gcgataccgt ccctgccgta aagatgttcc ataagatacc caaagtcatt
     4981 gatttccaga cggcgaacct taaagcgacg ggagatttta ttttccagta acttttccat
     5041 tttcatgtaa cggttgattt catcattcgg catggaaaca aagtcgttca tcagcgtgtg
     5101 gttcacttca tggagaaact ctttgaacgt cagccacgcc gattttttaa ggttcttcag
     5161 attgagctgt tcttccgtaa ccatgagttt aaagccaaga aaaaagcggt agtccacttg
     5221 attgtcccca atcattgata ctaacgcttc ggtctgttcg tctatcttct ggtaagcgac
     5281 ttcctttaat ttccccgtaa ccaatctttt tgactgctcc tgcatacttc ttatagagct
     5341 ttccgtggca atctgcaaag catgaatttt accctcacgg gactgtgcga ttagctgtcg
     5401 gaaactgtca tgcacgataa atttctgttc tgcggataag aaagaatagt tatacggtat
     5461 cagctcatag taggcgaaca cctcattgtc cttgttccag acaaggttgt tgtcaatata
     5521 tttaatcggg aacataactc acactcctta ctgccgtgat tgtttcgttg aatatttctt
     5581 tttgcagttt tacggctttt cctgcataag tgattttcgg tcgcaacgca taggttatct
     5641 gtgatttcaa aaagctgtat ggcttctttc cgtcaaaggt tttctgcgac ataaaccaag
     5701 tgaggaaagc aggaataccg aagtatttta aaaatgctcc ctcaatcatg gaaagtgggg
     5761 gcatatcccc aaacagaatg atgataaatt ctgtaatcac aaaccatgta atctgtgtaa
     5821 aggtaacggg aaagggcaag ttaaagtcat tgattgcata caagactttt tctacattcc
     5881 agatacccgt gtagcttcta atctttttca agttctttca actcctctca taagtttcta
     5941 aatggaaaag gacagccctg tttcagactg tcccttaaag agaaatacgc cgtcagtagc
     6001 aacaatgctt gtcgctgatc ttccagcgtt aatatggtat ctcgcacatt cctcggcttg
     6061 ttgcaatgaa gcaaccgccc atatccaaat ctcgcccgta tgcttcatag tcaatatagt
     6121 tctgcaagct agggggaata tcgccaagtg cctgcaattc atctatgtag taataggcaa
     6181 catctgtcat ggtttcacag tcgggataat agtaaatatc gtccttgtgt tccacgactt
     6241 cctccagcgt cccataatga ccaataaatt cgtccaaaca atccacgata tagtcgggaa
     6301 gttcctctat catttcatac atttcattga gttcatcaat ggaaacatac tccccaatct
     6361 caatcgggaa gttatcggta tcatggattg cgtattcttc atactgttca ttcaagccga
     6421 ttttttcttt cacatcttcc tcgtcaatag ggaaagtaaa ccaagcaccg actaaatagc
     6481 cctcattata cttgccaaga ttagcgatat aaactgccat atcatcaatc atacttactc
     6541 tggcaattca aagataccgt gttccgttac taaaaatttc ccgtgttcct gcaagtgtga
     6601 agcataggtt tcaaagtcaa aatagtattc ttgacagtcc tcggataaat gtttgaatgt
     6661 agggtcattc atcagctttt gccttgcaac atcaatcatg cttttacagt cgggataaag
     6721 cgtaattaca ttcctgcaaa tataaagtgc ttctaaattt tcatacaccg tatgaagcat
     6781 tgtatattct tcctgcatat cgcttgaaag ctggcgatac atataatcta attcattgag
     6841 ctggtacacg cttgtatgct catggacttc atcagcataa ggcaacaatt tttcaataat
     6901 gcggtaatct tgactttctg tactgacacc taacttttct tcaaactcgg caacctctat
     6961 cggcaggtcg aaccagtatg atgttgtttc ttcgccagtt gttcttttcg tttccaccag
     7021 tacccttgtt tcctgcattg ttcctcacgt cctttctgtt gtttcatcac atcttttaaa
     7081 gtctggcatg acataccaaa cacatatttt gaaaaatctt ctaactgttt ttcttcatag
     7141 tcggcaaggt tcagttgctt catttcatgc cacaccttac gcttgtaaga gggcaggtct
     7201 gaaatgtttt cacaaccaac ttttgcctgc atatctctaa aaatatcgtt cattacatca
     7261 ctccaatctg aaatttaggt aagaaaaaag cagtcaatcc taagactaac tgctttgtgt
     7321 gtatggatat gaagttgtca aactgggatt tatctggcag acttgtttat ccactcatca
     7381 agaaatttca tttgttcttc cgtgtgaaac caatgctctc cgtttctcat aacagtaagt
     7441 gttgcattag tttgttttgt aaattcagat atagttgcat aagaagtcaa gttatctttc
     7501 tcaccaaata aaatatttgt tggaattttc catgtagttg gattttcttt tacataacag
     7561 agatattccc atgagagtgt ttctccaaaa gttgttttaa tttctttttt atctcgaagt
     7621 tcatcttctg taacatttgc ccaatacatc atatctgcaa tcagtttttc catattcaca
     7681 ataggtgaaa taaaatatgc tttttctatt tgcttatctg ataaggcgtt catagcgaaa
     7741 taggcaccta tgctatttgc aactatttga acagatttgt aatgtttgca aatcaaatca
     7801 aaaagtgatg gaaattcttc ttttgcttcc catggatatt gtgcagtata gttgagtcca
     7861 accacatcac aatcactaaa aagaggtttg taatgtgaag actcctctgc atttccacct
     7921 ttaccatgta tataaataac tactttatcc ataatagttg tcctcctcaa cttcaaattt
     7981 gtcgctttct tcatatccac attatatcat tctcatatca gcaccacaat aaaattttac
     8041 gcaccgataa tcttattgaa cagctctaac agtacgtctt tcacaccgcc agcattgaat
     8101 accaatccaa cggcaacaag ggcaactacc aagaagccga taagtttaga aaactcacgt
     8161 ttgaacccta agtagatacc aataaccaca atcgccataa gcactaagct ctgggcgttg
     8221 cttaaaaacc atgtgtatat ggataggtaa gttcctgata ttataaatat ctcaaacttt
     8281 tccccccatt cacacggagc gtgcgacttt caacgcactc cgcgttccat cgtgtttata
     8341 ttgaaaagta tttatgtagt agacctatat caagtttaat tcttgattgc cagctaatac
     8401 tctatattta tttagtctta tgagctggac tttatttagc ttcaatagag ctatatattt
     8461 ttgtattgtt tcttctgttt tagcgtgtat tagacggtgt accgcctctt ttaccaaaat
     8521 atgattttta taatcgtcat ttccaccagc tccagttggt acaatatgat gacaatgtat
     8581 ttcattagct tgttgaaatt cttccagagt tatagcacat tttccatgtt gagcagaaaa
     8641 tagcgaaatt cgattgtctg cgtattctat tgtgcgattt tccaaagata atttcattaa
     8701 ctggtacatc agtaatttat caacacttag attggtgtgg ataaattttc ttccctctag
     8761 agtgtagcaa ttcgctaaat acacctttgc cattggtttt ctgtgttgga tatatccaac
     8821 agggaaaata ggttcgccct cgccagcaac ataccggagc attttagatt taccatatct
     8881 tgttgcctcc tttgtcgtaa gttgtctgcc tgtttttctc aatctgcccc ttttgtttgt
     8941 acctaatctg ttagtgaaga tagccattac agctctattc agtttcatac aatccagatt
     9001 tacattcgtt gcaatctgat aataattttg catacctaat accattgaat tataaagtct
     9061 tgtttcttct aattccgttt tcccttttga gggagaagca attttctttg cctgtgatac
     9121 cagcttgtct ttttggtttt tgaattgttt atcacttata tgtgatttaa caactaactt
     9181 ctttcttctc ggtatcattt taatcttgaa accaagaaag tcggagtatc tttttctaac
     9241 atttacaatt cttgtttttt cttgtgatac ttccaatttt aatcgttgtt caatccattg
     9301 agtgattgct attttggctt tttcagcact ttccttgtag cggcagaaaa ctctaaaatc
     9361 atctgcataa cgaaccatat acatttcctt taactttgtt ttcttcattt ctttgtaagc
     9421 gttgctttta catgggctac cgcttttatt gatatgaacc acatagtttt ttgtaacagg
     9481 attttcctgc cattggcttt ctacccaatg gtctaactca ttaagaacaa tgtttgctag
     9541 tagtggggat attattcccc cttgcggagt tcctttctct ggatatgtta ttgtaccgtt
     9601 agtcattttg attggagctt ttaatatcct ttttaaaatg aatatcaagt gcttgtcccg
     9661 tattcccata gcccatatct gttttatcag cttggaatgg ttcacgttgt caaagaaacc
     9721 cttaatatca aattcaatca cataatgtag ctggctgatt tgtaggcggt tataggttgc
     9781 ttttattgca ttttcaactg acctattagg tctgaaacca tagctgtttt cactaaattt
     9841 agcttcgcag attggttcta atacttgttt tatacattgt tgtataagcc tgtcccacat
     9901 acaaggaata cctagaggtc tggttttacc atttggtttt ggtatttctt ttcgcctcac
     9961 tggcttaggt cgatacccat gtggactgcc tgcaacaatg tatcttactt ttttgacaac
    10021 tgtttctgct ggcaatttgc caatatcttt aattattgta ccgtctgttc ctgctgtttt
    10081 actgcctgca tttgctttga tatttcgata tgccagtaat atattttctc tcgataggat
    10141 aatatccata agatgagtga aaatatctcc attcttactt ttggcatata aatcatcaaa
    10201 ggtgttttgc atttcgtaat attcagcgtg tcttaaatct tctacacaca atgtttcatt
    10261 tttattttta ggcataaggc ttcactcctt tgcttgaaat tgagtgagct tttttatctt
    10321 actcgtaatc cttattgaaa caacataata cttttctaat atatacacga cttgagggca
    10381 ttcctccatt ctcattacaa gaatttctta ggttcgccct ctgcttttca gcattagttc
    10441 aactgcttat tgttcttcgt cagtttattc tcttgatgag aacccaatac ctttcctcgt
    10501 tccgataatc ttatctatac atatatatcc ttaggtcact agctataagc ctgctggctt
    10561 gataccgcct gtaacgttat atggcatttc ataacctata ttcttacttt gccacaccag
    10621 ctacgatttc tcgtaccttt gcatttctac aaagttcaaa tttagacccg tacattcgct
    10681 gtttcgtcag ttccttatag aacattctaa ccatagatat tttatagacc cccgacctgt
    10741 tggatacgct acccgcctct ggatagtttt cgatagaaga tatatgttca tcaactatta
    10801 cggtatcttt cagccgtctt taccgagctt caaacattct gagtttcctc atacaatgct
    10861 tgtcggaggc tcagggcagg cgtttcagag cgttacttca tcattccacc tgtcagatta
    10921 tcagttctcc taataaccaa actttttcgt tcttttatta gtgccttagc ttttcactaa
    10981 ggaacgagtc gcacataaat tttgtccgaa attcatttaa ttgtcctctc tttctttgat
    11041 ttcttccata catctgtcag cttctttgcc gacagttaaa atgcacatca agactacacc
    11101 gatacccatt ccaaatgata ccagcaataa gtctttcaat aattcccaca ttttttcatc
    11161 actcctttac gttggcaacc atttcttcaa ccgtggctgt ctgttgcttg attatctggt
    11221 agtgtcgttc ggtcagtttt gcctgctttt caatcgtttc cagatactcc gtctgattgt
    11281 ccttgtctat ctgcttcaac atcttcaagg tcggtgcaac ctgcctttgt agccagttga
    11341 gtgtacgctc cagcgtgtaa ggttctggct gtgttgtcag ctttacgggc ggtcgcccct
    11401 cgccaatgaa ccaagcccag cgtacattgg ttttccagtc gctttttcgc ttgtctggtt
    11461 ctttatcagc gaagcggata tattgattga taatagaaaa tgctgtcagt tctgcgtcat
    11521 aatgtgtcag cagttccaca acagcataat aggcacgttc atttttcaaa cgtatctcaa
    11581 atctattttt tatggggact tcctcaatcg gtatgccaag tttggcgtac tgttcgtaat
    11641 tcttttcata acagcagaag tacacttcac ttgaaaatga accaatatac agcgtgtgtc
    11701 ccataccgta tttatccgtt tcattatgct ttaccagctc tccagaacca tagcttttga
    11761 aagaacggaa tttagaaata cattcctcat tctcgcattt tcgtatcagc tctggaatat
    11821 caagtaagcc cgttcggtca ttgattgcca aatcaagacg cttcattaca ccgccctcaa
    11881 ccaatgcgtc cgtgaagaag tcataccaac ttcgctcttg tgccagcaga tagctttcaa
    11941 actggcgaca gcctttgcct ttcagttcca gcaggacacc tttttcttcg tcctgcgata
    12001 catacacaaa cacatctccg atatggtaat gctcggtgta tttgtaatgt ccgtaatctt
    12061 cgtgaagcat atagccgatg ttgagcttca atatatcctt gatgatatgc tttatatcca
    12121 ttgtgggaaa acggatacgc acataatcaa acagcaggaa catgggattg ttgggattga
    12181 atttttccac cgcttctaaa agcgtatgtt tcatgtcctc ggtcagcgtt acctttcctg
    12241 cttcgatacg gctcaaatgt tcacggctga tatttgccat gattgcaagc cttgtctgcg
    12301 atacgccgta tgcacagcgt ttttctttca aggcggttaa aaaatcagtt tcattcagtt
    12361 gtcatacccc caatcgtgat aattttgtga tattgtgata atttcaaggc tttatcacaa
    12421 aaggcgaaat gtacgaaaaa cccttatttc atgcggtctt gcaaaggttt ttgtggaagt
    12481 ttccgccgta attgtgcccc tctgttagat aatgaggggc ttgtatctgc tggcgtggct
    12541 aacgccacac cagcaacggc tagtccgtgc cgtcgccttt cgcttcgcac gtcgccgtcc
    12601 cgtcctgcct tgcgtatgca agtgagccta tggtctgcaa aaaatcatgt ccttttggaa
    12661 ctaagggtgt gtaaaattca cttatgacgc ttgttcccac atcacaatag ccacgtccct
    12721 tgatacgctt ctggaaaaac tgttttttca catcagaact gaacagcata ccgtaaccta
    12781 attcgctgat acgccccaag ccaacacgga aattaaaatt atctctgata ccgtccgaga
    12841 aatattttgc gtctggacgc tggcaggcaa caataaggaa atatcctgct tgtcgtccta
    12901 acatgacgat tttctttagc tggctaagta ggctcacact ttcttttgtc cccagcattt
    12961 caaaaaacgc cacatattca tcaaagataa gaaagcaggg cggtaaacca agataggcat
    13021 agttttcgcc cgttttatag tctgggtgtc gcttcatttc ttcgcttctt tgtaccatgc
    13081 cctcataaaa ggcattgaca caatcaatca tttcttcttt ggtgtgatac acatttccca
    13141 ttaccgtacc taaatctgca aggtcagcgt tcttcgggtc taatatgtaa agaacagcgt
    13201 tcgtatgtaa cagagcttca ataagcgtca gcagaaaata cgttttaccg ccaccagtcc
    13261 caccagcaat cagagcgtga gggagtgcgt catattccca tacaagattt ttcatcagcc
    13321 taagacagcc gttttctgct ttcacttcat caatggtaat gcggttcgct atcatatcat
    13381 aaagcatggt gtattcgata taaccgtcat gtaacgtctt gtcggtcagc tcacaataca
    13441 agccactttc caatttatcc tctaaccgta agagctggtc ttgatatttt cctaatgtaa
    13501 tttcacagcg gatatgaagc agtccttttt ccatttgata ataaatcttt ggaaaccaga
    13561 tgattttttc ccttgatctg ctttgcaggt cagtaaaaaa accgctgtct tgtacggtgt
    13621 ctgcttcata ccacttgttt tccagtatca ttcgtgccag cttttgacgg tgcaggagct
    13681 tcttgaaact gtcataacag aagcggtaat acagaaaagc gaccaatgca caaacaccag
    13741 tcgctatcag tagggttatg aagttgtaag tggaaagcgt caaaccgttg tctaataagc
    13801 tgaaatgctc ccaatcggta cgcatgagct gtttcatatt cagtagcagg ataaccgcaa
    13861 tgaatacaaa cagaagcgtc cctatggaaa agtggtagac aaggttcttg tcgctggctc
    13921 tgatacggtg tcctttgttt aaaatcattt tcataaatca atcactcctt tctgggtacg
    13981 aaaaaagcag tcaatcctaa gactaactgc tttgtgtgta tggatatgaa gttgtcaaac
    14041 tgggatttat tgttcttaac ttttctgtcg ttataaagct gacttacgca cattaacctc
    14101 tgcacagacc agcccagcta tatgctccgc tgtttcattt gtcagtacaa agagcattcc
    14161 gtcattacgg cattttttat gtgtgtcgcc attcaaggca taaacaaaac cttttacaca
    14221 ggtatcacta caggcattag ggtcaatcag tctcttgcag tcacgagaag tagtcatttt
    14281 cttttgcata tcagcaggaa gcgaagcgat tatatcctca tactttccag cgttgtctcc
    14341 gtaaatccgt gcaaaaacac ctgtcttgcg aaacacccaa ttcattactg ttttcttctc
    14401 tagttggtat gaagcggtgt aaccgctttt tgcttccttt attacaaggt cacagccctg
    14461 ctcaataagt ttggtattaa gtttctcgac aaacgcttgg tgttctggcg cgattgccga
    14521 tagaaattct ttgaatgaac ctttttcttt tgccattatc tttttcctcc acaaatttca
    14581 atttatccgt ttcttcatac acatattata acagttgctt atatccgcta caatgaaaat
    14641 ctagtttgcc agcttatttc tttggttgcc cctgcggtgc atggttctgt ggattgccta
    14701 cttgaccgcc tttattcttc aagataatat cgtcagcctt gatataccag tccacattga
    14761 cacctcgata gctggcgttt gctaccgtat cggctacggg attgacaagc tctacctctg
    14821 cattatacgg atagtccttg acgggaatat cagcaggaat tgatacttgt atcatacgtt
    14881 cctgcacact acatttcaaa tcataggttc ttcgtgtgat ttcttcactt gttgaaccgt
    14941 cgtcattttc tgctcgcacc tcatgtctga gggcagagaa cttcaataca ccgaaagttt
    15001 tttccttgtc taatacaata ccgtttgcta atctcataat catttacccc cttacttgct
    15061 ttcctcggct ggcaacatat catcagccgt taagatatag tttgtgaacc ctcgtccttt
    15121 gaccgcttta ccgtctgcgg taatctttgg attgataagt tttactcgct gttctggttt
    15181 gaaatgcttt tcgcctgctt tcgctggaat ggttacgata atatcatcag ccctttgtac
    15241 gtcagagtac aagttgtagc ttcttgatac gggcgtaaag cgtccgttaa tgcgttgctg
    15301 ttcaatcttg ttttcgccag caaattccaa gtttccaaaa gtctgtgcca tgttcggcac
    15361 tacatatttt aattccatag tgttttacct cgtttctttc ttctttttta agtttgttga
    15421 ttgtgctttc ttattctggg aattagggaa ataccagtaa tcccccagat agtaaagggt
    15481 aaatcaatgc ttcgcaccct tgactatccg tgtgcttgca ggttgttggc agacaagcca
    15541 gaataagtaa acgtctgccg tttgacagct tcggcagaga gcgtgatttt tcttttctca
    15601 taccgttacc gccttatgat ttcttcattt tacggcgttt gtaaagatat gttccagcca
    15661 gtccaccagc agatgtaagc aacattacca gaaatgccat gacatttgtg ttgtcgcccg
    15721 ttttaggact gtctgttggt ctgctcggtt tttctggtgt tgttggcgtt tcgggtttct
    15781 ctgggacttc tggtttttcc ttaaaagtaa tcgtctgtcc ctcgtcctca atgtccttat
    15841 gctcagtaac ttttttcggc tcgtttggat tgcttaaatc atacaattct tcaaaagtta
    15901 caagctgttt gccgtcaaga gaagtagcgt caaaggtaaa ggtaacttcc actttcatag
    15961 tttcgctgtc agcgataaac gtataatcac tttccacacg ttttccattt ataagaagct
    16021 ctgcattttc ttctttcaac atctgccagc ccacaagttt atactttgta ccaacttcca
    16081 agccctctaa ggttacagta tcaatgattg ttacgtcttt tcctgcttca agctctttgt
    16141 taccgtcctt atcggtagca gttgtatgta ttttgatgat acgttctgta ataagtaccg
    16201 tctgcccgtc gtcctcaata tctttatgct ccgcaacttt catgggttcg tctggattgc
    16261 ttaagtcgta taattcctcg aatgttacca gatttttacc gccaagctcg gacgcattga
    16321 atgtataaga aatttccact ttcatttctt catcatcagc aacaaaggta taatcgcttt
    16381 ctacacgttt cccgtcaatg ataatctcgg cgttttcttc ctttaacatc tgccagcctt
    16441 ttaactgata cttagtgcct tttgtaagtc cgtctaattt gacagtatca acgattgtta
    16501 cctcttttcc tgcaaggatt gtcttttcgc cgtctttact ggttgctgtg gtatggatag
    16561 tgatttcctt ttcgtattca tcagtcaaag tccctaaatc aatcacaaga ttatttcttg
    16621 ataccacgat ttcaaatggt gggataagtt ccatgccttt gttgctgtca gagcgtaatt
    16681 cttcaataat gtaagtatcg taaggcaacg cacctttgct gtcgtctggt tcagaagttc
    16741 caaaccatac accgtcctcg cttgttttgc cagcgttggt attgtgcttg tgcgaagccc
    16801 attttgaaga agtggagaat tgcccattat cgtcagttac cacaacatga ctttcgcccg
    16861 ttgtcttgct tgtgatccta aagggaacat cagcaagacg cttgtgtgtg cctgcaccga
    16921 ttttcacgcc ctcaatatcc ccacgcttaa tctgattgta gatagagtgg gcttcgtctg
    16981 ttaagtccac gatttttccg ttttctgtga ttgtaaaatc aatcggcttc gcaccgtctg
    17041 tcaagtaacc gtttggagct tgtttctcaa cgatacggaa attcccgtaa ggcaaaaggt
    17101 cagcagaagt agaagcgata ccctcaatat ccgtatggat tgtttttaca acttcgtttt
    17161 tcttgtatat cttgccctca accaataccg cattatcatt taaggaaatg atgtcaaagg
    17221 cagtatcttt caaagtggca cttccttgtg gtttggtatc tttggtttct aaatctcgtt
    17281 tctgaatttt gacaccgcca cgaatgactt tatctgatac ggaaaactgg ttacttccag
    17341 ttaatacggc aaggtcgccg tcctcggtaa tctgcgtaag gtataaggct tttatctgtt
    17401 cttcactacc attcgcctgc atatatgcac cgtctagtaa gtagcccgtt ggcgatttcg
    17461 tttcttcgac cgttagtgta ccaagaggta aaaacgcttt tccgtcctgc atatagaagc
    17521 tatcgccaga taccttgtat gcgtctgcta attttgtgat gtaatgggtt gtcccgttac
    17581 tgtctgtttc agcggttgtc tttgtaaccc atgtacgagt agcttcggca ggaagattgt
    17641 ctttattgta gaagcctgca taatatttcc atgtaaactc cgccccctct aaagaagcgt
    17701 tcccttgtgg gttggctttc tgtgtttcca tgtcaatttt aaaaagctca atcaaagtat
    17761 cggtaacttt tggcgtatca gatactttca aagtcgctgt ctttccagct tcgactttta
    17821 aggaatacac cgtagtatca actttatacc ccaatggtgc agataattct ttgatatata
    17881 cggtagtagc tttgacttct gcaacctctg tatttccgct gtcgtcagtc gtaagggtag
    17941 caatctgttt ggtgcaatcc ttatcggaaa agacaccgta agtcgcacca gcgatagagt
    18001 aatttccgtt accgttagta atgctggtat tattggaagt cttttgaagt ttggcatttc
    18061 cgacatttaa cttcgcccag aattgcccta attcctgccc ctcgccagag tagatataac
    18121 cgccgcattc atagcgtcct ttgttttctt tcacaaaggc ttttgcacca gcaaacactt
    18181 catcttgtgt tgcctttggt atttcatcat aagaagctcg cacgttatca cattgccagc
    18241 ctaagtgaac actcaatctt tgccacacaa caagctgtct taaaaggtag gcgtgatttt
    18301 tgcttatccc attgtggttg tcggtgtatt gcttcacata ttcgatagat aaggcaacat
    18361 cacttatctg gtcggcactc atacgggagc ttgcgtcagc tctggtcttg tagccgttct
    18421 taaaatctgt gttaatatcg atacaataag cgtcctcgcc ctcaacggtc aaatgcccct
    18481 cgttaaaggt agaaccaatc gaaccgtcat tcattacttt ttcaacgata ccgacacgtt
    18541 cttcactttc cgtccagtat tgcttgcttt ctgcatggac aagtgtagtc ggtaaagcag
    18601 taacgacagt cgcaaaggca agtacgcccg tacacaatcg tttcaatatc tttttcataa
    18661 ttctaaatgc tcctttcatt ttgggtaata aaatagccgt ccataatgaa cggctggaaa
    18721 tagaaaagga acgtcaattt aggcgttcca tagtctatga aatattcaat ttttttcttg
    18781 cttcaatact gatgtgccgt accatatctt tgcatatcta ggaaaacttg cgtatcaagg
    18841 ctcattcatt ggtagtaaat tagtagtaaa ttgatgtgtt tgtttccttg aaaatgctga
    18901 tagatactat gttttagcgg ataatcaact ttttagtatg ttttttgcat aaaaattgag
    18961 tagatagtag ttttattgta cttaatgatt gtatttaagt gattagattt gaaattgaat
    19021 aatgtagata ataaaaaaat attaacagaa cttaataaac tccattaata tttttttatt
    19081 atttttgaaa ctgtgataat tatcacggaa gcatagaaaa taaagtgtta agatttaaac
    19141 aagatagtaa gtaagtaaaa ctaaaatata agaaaggtga taaaaatgaa actaaaatta
    19201 gattcatcta agttcaatga aggtctgaat tatctaaaaa aagactacaa gatatttgca
    19261 ccagttttaa taccatttaa aggtactttt tcagatactg atgttataag atacaaggag
    19321 gtaaacgctt ttgaagaaat ggaatttgct aagaaagcaa acttttcacc aaaagatgtt
    19381 gtacttccca taaatcaagt attgttttat tttactgaaa aagagtttaa ggaaagcgat
    19441 ttagacaaca agaaaatttt aattttctta agagcttgtg acataaatgg tataaaaaga
    19501 cttgatgaaa tatatctaaa taatggtgaa gaaaaagatt atttttataa aaatatcaga
    19561 gataaagtaa agtttgcact tgtagggtgt aaggaaagtt ttagaaactg tttttgtgtg
    19621 agcatggatt ctaacaaaac tgataattat tcaattggat taaatataga aggagataca
    19681 ctgtacttag atataaagga tagtgaattt gaagtattta atggagaagc aactgaattt
    19741 aatgttgatt atgtaactga taatttaata tctgtagatg tgccagagaa tattgattca
    19801 aatgaaatga taggaaattc aatatgggat gaatatgata caagatgtat aggatgtggt
    19861 agatgtaatt ttgtatgtcc aacatgttca tgttttacaa tgcaggatat tttctataag
    19921 gaaaacgaaa atgtaggtga gagaagaagg gtttgggctt cttgtcaagt agatggatat
    19981 acagatatag caggtggaaa ttcttttaga aaaaaacaag gcgaaagaat gagatttaaa
    20041 actatgcata aaatacatga ttttaaaaaa agattcggat atcatatgtg tgttggctgt
    20101 ggaagatgtg atgatgcctg tccgcaatac atatcatttt caaagtgtat agaaaaaata
    20161 aatgatttag taattagtaa ggaggaagta taaatgaacc catatatacc agtaagtgca
    20221 aaaatactag atatagttaa acatactgat aaagaatgga cttttagagt aaactgcaat
    20281 acaaaaggtg tattgcctgg aaagttttat gagatatcta tacctaaata cggtgaaagt
    20341 ccaatatcag tatcaggata tggagaggat tatattgact ttacaataag aaatgtagga
    20401 aaagtaacta gcgagttgtt taattataaa gaaggagatt ctttttttat aagaggtcct
    20461 tatggaaatg gatttgatgt atccctttat gaaggaagag aaattgtagt agtggcagga
    20521 ggaagtgcat tagcccctgt aagaggaata gttgaatatt tttataataa taaagaaaaa
    20581 tgcgaaaagt ttaaattaat agtaggattt aaatcaccaa aagatatttt atttgcagat
    20641 gatttaaaga gatggagtaa aaaacttgat attttagtta ctgtagatgg agcagaggaa
    20701 ggatatagtg gtaatgtagg attagtaaca aaatacatac cagaattgga tatgaaagat
    20761 ataaacaata cttctatagt tgtagttgga cctccaatga tgatgaaatt tacagtagaa
    20821 gaatttttaa aaagagattt agatgaaaag aacatttggg tttcttatga aagaaaaatg
    20881 tgttgtggaa taggtaaatg tggacattgt aaaatggatg atacttacat atgtttagat
    20941 ggaccagtat ttgattattc atatgctaaa aatcttattg attagggggt gaagtttgtg
    21001 ataagagatt taaataccaa aaaagtaatg aaaaatgctt atagaattac aaaaacaaaa
    21061 tatgagactt ctttaagagt tagagtacct ggaggtctta ttgaccctga gagtttagct
    21121 gtagtttcta aaattgcaag tgagtacggc aatggacagg tgcacataac tacaagacag
    21181 gggtttgaaa tattaggaat aaatatggaa gatatggaag aaataaataa aattatacaa
    21241 cctgttattg aaaaaatgaa tataaatcaa agtgagaaaa acgcgggtta tccagcagca
    21301 ggtacaagaa atatagcagc ttgtatagga aataaagtct gtccaaaggc tcaatataat
    21361 acaactgaat ttgctaaaag aatagaaaaa gctatatttc caaatgattt acattttaaa
    21421 gtggctttaa ctggttgtcc aaatgattgt ataaaagcta gaatgaatga ctttggaatt
    21481 attggtatgg ctttaccaat atatgaaaag gatagatgtg taaattgtgg agcatgtgta
    21541 aaaaaatgtt ctaagatatc tgttggagca ttaaaaacag aaaataataa ggtggtaaga
    21601 gataaagata agtgtatagg ctgtggagag tgtgtactaa attgcccaac aaatgcatgg
    21661 actagagatg agaaaaaata ctatagatta gctataatgg gtagaacagg aaagaaaaac
    21721 cctagactag cagaagattt tcttttatgg gtagatgagg acagcataat aaagataata
    21781 cttaatactt atagatatgt agaaaagtat atagataaag atgcaccagg aggaaaagaa
    21841 catataggat atataattga tagaacagga tttatggaat ttaaaaaatg ggcacttgaa
    21901 ggtgtagagc ttccagaaat aactaaaatg tacgaaaaca tatactggag tggaattaaa
    21961 tatctataaa ttgagtaaaa catatatttg aataaattaa aatttaatag aaggaagagg
    22021 gagttatgca taaagaaact ttggataaat taactaatgc agctataaat aagataaatt
    22081 tattaaacac aagtaaagta aaatatttgg tatcttctgc gtttgcagga ctttatgtag
    22141 gtataggtat acttttgata tttactattg gaggactttt gactgatgca ggttcaccta
    22201 tgactaagat agtaatgggg ttatcatttg caatagcact aagtttggtt ataatgacgg
    22261 gaacagagtt atttactgga aacaatatgg ttatgtctgc tggtatgtta aataaaggtg
    22321 taagtataaa agatacttct aaaatatggg catacagttg ggtaggtaat ttaatcggag
    22381 ctttggtttt aggcattata tttgtaggaa ctggtctagt agacaaagga cctgtagctg
    22441 aattttttgc aaatacagca gctagtaaag catccatgcc atttacagct cttttcttta
    22501 gagggatttt atgtaatatt ctagtatgtg tatcagtgtt gtgttcgttt agaactaata
    22561 gtgatactgc aaaaataatt atgatattct tatgtttatt tgcttttata acatcaggtt
    22621 ttgagcatag tgtagcaaat atgacaattt atagtgtttc attattttca ccaacaataa
    22681 gtacagttac tattggagga gctatttata atttagtagc tgtaacactt ggaaatattg
    22741 ttggaggagc tttatttatg ggtcttggaa cttatatatt aggtaaagaa aagcttaatt
    22801 aaatttattg tataaattat aactctcttg ataaatacat ttttagtatt gttgagagag
    22861 ttatttttat atttttgaaa aaatatttga aacaaaaaaa atatgggagc atacgaaaat
    22921 atatcggtta tatatataaa aaatacgttt atatatgaat tatatagttc taaatataaa
    22981 aaaagttaaa gtcctaccgt aaaaaagcgc attttgaata agataacaaa tatgataaaa
    23041 ttggtcttgt tgaaattttt aggggggata tgatgacaaa gaaactagta atatcaaaaa
    23101 caaagttgtt tatttttata atagcattta tttctatatt tatatattta tttggaagta
    23161 aaaatacact tataggtgta ggtatagtaa cagctatgct aacactcttg gaaagagatt
    23221 taactatttc tccaattaaa aatttactga agtatttagc aataaatata atcttaggaa
    23281 tactatcatt ttttgcagta caaaatatgt atttaggagt tttattaaat tttatagcat
    23341 tatttataat agggtatgta tttagttatg acttaaaaag agcagtatat gtaccctttg
    23401 gtctaatgta tatatttatg gtaagtatac ctgtaggaaa aagtgaattt cctatgagat
    23461 tgtcggcttt agctgttgga gcagttataa taatgattgc tcaatttgtt atgaatagaa
    23521 ataggatgaa aaatgtcgga gataaagaac tgatatctat atgtgatgaa ttattagaaa
    23581 aaattagcct tttaaaaaat actattaatg atgatagttc tataattaaa tctagtatga
    23641 gaaaaataga tagctgtaat tatagaataa attctatatc taaaaatttg aaaatggtta
    23701 tttttgataa tagaaaagat gacttttata tcagtattag aggtatagac ataatgaata
    23761 ttttattttc attagaaaga ataagtttaa tcctggaaag atacaaaaag gatagtaaag
    23821 aatttgaaga tgaagatata aaaaatattt tagaagaaag tagcataaat agtaaatcag
    23881 atgttttagt tgtagcaacg aaagaaataa actatattaa gatgtgttta gaaaataaag
    23941 ataccataac tgataaagag atattaggat ttagagatta tgttattgct caggatacta
    24001 aaaatataaa tttaaaagaa atttatagtg ttttagaaaa tttatatgag tttttagtag
    24061 attataaaaa agtaaaatca agagacgaaa agaaagctga aagaaaatca aagatacctc
    24121 atgaatttaa acgactatca atatataaga aaaactttaa tttaaattct ataagatttt
    24181 cttatgcagt aaagattgct ttagctacag ctgtagcggg ttttattatg gattatttcc
    24241 atttaagaga tggaagatgg ataatgttaa ctgtcttttc attaacacaa ccttacgcag
    24301 aaaattgtat acaaaggtct agaaaaagaa tagaaggtac atttatagga gctgtaatat
    24361 ttattgtatt attttcaata ataaaagata gtacattaag gtcgttaata gttcttttag
    24421 cagggtatat aaactcttat gtagttgatt atagaaaatt aatggtatgt gtaactgttt
    24481 cagctcttgg ttcagcagta gttatgggag acccaaatgt tttaacaata agcaggattt
    24541 catatgtagc attaggagca attattgcat taatagtaaa taagtttata ttaccatatg
    24601 atgctaaaac aggctatcaa catgttatag aaatgtataa aggaatagtt aaaaatataa
    24661 ttgatgaagt caataagtct atagaaaatg tagctgatgt ttactatata aaaaatttat
    24721 tattgatacc atcacttatt gaagatagac ttatgttgat aaatgctata tataaagata
    24781 aacatcaaga ggatttttta gaaaatcaga aattactaat aagtaatatg tataatctat
    24841 atataaatgt taataaaaat aaaataaaag atgaagatgt tgaaaaaata ttgagagata
    24901 ccaattatat ttcaaattac aatgtagata agtatgatga aggaagaagt gtaattttag
    24961 agagtattgt aaatactaaa agtctaggtg acaagataat atgtctaaac ttactgcaaa
    25021 ctctaaatgg tgtcaaagaa atgtatagaa taagtaatat aactaaagtt tcaataaaag
    25081 aagcagctta gatattaaat tatagaatag ccatatattt aaaataaagt atatggctat
    25141 ttgttttata aaatactaaa agaaaataat atataaaaaa caaaaaacat atatttatga
    25201 attttatatg aaaatagtta aaatattgat ttttttcatt ggttgaaaaa acaatttact
    25261 taggataaac tataatagta ttcaaatatt atgggggtta gatttatgag taaagatgta
    25321 aaggtaaagc gtaataagaa atatgataca tttagtataa ctatggaact tgagagacct
    25381 ttactttatg atgaggttga gaacaaagat gataaaaata ataatagaaa atttaacata
    25441 aagaataaga tatttaaggg aataggtata ctatttgtat tgattttgct tggctttttc
    25501 atatggataa ataaaacata tgaacctcaa aagctggcaa aagaagctct agtcagcaat
    25561 agtaaagttg aagtaactgt aaatgaaaac atttcattta caccaaaagg aagaaaagtt
    25621 tctaaaggtt taattttgta tccaggagct aaagtagaaa tagaatctta tgctccactt
    25681 gcaagaaaaa tagcagaaag tggatatgag gttgtaatcg taaagatgcc acttaatcta
    25741 gctatacttg gaactaataa ggcacagaaa gttatggatt cttacaacaa catagaacat
    25801 tgggtaatag gaggtcattc attaggagga gttgcagcat caaattttgc acgtgataat
    25861 aaattaattg aaggcgttgt atttttagct tcttatccaa tgggtgatga gttgaaagaa
    25921 ttaggaaaaa aagttatatc tatatggggg agcaaagatg gagtagtcaa ttttaaaagt
    25981 cttgtagaat caaaacaaaa acttcctgat gatacaactt atgttgagat tgaaggagga
    26041 aatcatgcac aatttggaga ttatggaaaa caaaagggag ataatgatgc tattataagt
    26101 caagaaaaac aacttaatat tactgccaat agtatcatta aatttttaaa gaatatatct
    26161 taaactaaaa tttaaaaaat ataattttat aagttattat ttaaagtaaa tattaataat
    26221 ataatagaat gaatatttta ttacaagggg gattttaata tggaatacga tatgccactt
    26281 tacagaccac caagtgaggc ttatagtctt ataatacaag tcacactagg atgttctcat
    26341 aacaaatgta cgttttgtag tatgtacaaa tctaaaaaat ttaaaataaa gccactagaa
    26401 gtaataaaaa atgaaattga tatatttaga agatattata agcatgtaga aagaatattc
    26461 ttagcagatg gagatgcatt gataatacca atggagaaat taagagatat tatcttatat
    26521 ataaaagaag tttttccaga gtgtgaaaga attactatgt acggctctcc taaatctata
    26581 gagaaaaaat ctgatgatga acttaaagaa ttaagaaaac ttggtgttaa aatgatttat
    26641 ctagggcttg aaagtggaag caatgaagta ttagaagata ttaagaaagg ttttagtagt
    26701 gaacaattaa taaaagttgg tagaaaagtt aaaaaaagtg ggattaaatt atcagccact
    26761 gtaatagcag gtcttggagg aactaaaaaa acacatcagc atgcagttga tacaggaaaa
    26821 atgttaggag caatttcacc agattatgtg ggtgtactta gtttaatggt agaacctaat
    26881 actgaacttt acgatttact taaggcagga gagtttacaa tactagagga taaggctgtg
    26941 ttacaagaaa ttaaagagat gataaagaat atagatgcta atgaaaaaat tgtatttaga
    27001 agtaatcatg catcaaatta tgctaattta aaaggtgtat tacctgatga taaacaacgt
    27061 ctaattgatg aaattgatta ctatctaagt aaccaaaagc taaaaagaga agaatataga
    27121 agactatagt ttttgatata tcaatctata ttgataggta aatactaaga ttatttagat
    27181 acaaaaaatc atgctacatt aatagttgct tactattgat gtagcatgat ttttttatta
    27241 aaaaacaaaa tggtatattc atatataata tatca
//
LOCUS       DAMJWS010000055        27268 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMJWS010000055 DAMJWS010000000
VERSION     DAMJWS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.055"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(245..466)
                     /locus_tag="PC399_002375"
     CDS             complement(245..466)
                     /locus_tag="PC399_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593232.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(867..2144)
                     /locus_tag="PC399_002376"
     CDS             complement(867..2144)
                     /locus_tag="PC399_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2593233.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            complement(2128..2796)
                     /locus_tag="PC399_002377"
     CDS             complement(2128..2796)
                     /locus_tag="PC399_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593234.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            complement(2956..5457)
                     /locus_tag="PC399_002378"
     CDS             complement(2956..5457)
                     /locus_tag="PC399_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF2593235.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQNVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            complement(5748..5939)
                     /locus_tag="PC399_002379"
     CDS             complement(5748..5939)
                     /locus_tag="PC399_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF2593236.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            complement(6087..6329)
                     /locus_tag="PC399_002380"
     CDS             complement(6087..6329)
                     /locus_tag="PC399_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593237.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            6541..8364
                     /gene="typA"
                     /locus_tag="PC399_002381"
     CDS             6541..8364
                     /gene="typA"
                     /locus_tag="PC399_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF2593238.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            complement(8454..9362)
                     /gene="trxB"
                     /locus_tag="PC399_002382"
     CDS             complement(8454..9362)
                     /gene="trxB"
                     /locus_tag="PC399_002382"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2593239.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     misc_binding    10164..10395
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10503..11984
                     /locus_tag="PC399_002383"
     CDS             10503..11984
                     /locus_tag="PC399_002383"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF2593240.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            11994..12887
                     /locus_tag="PC399_002384"
     CDS             11994..12887
                     /locus_tag="PC399_002384"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF2593241.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            13011..14072
                     /locus_tag="PC399_002385"
     CDS             13011..14072
                     /locus_tag="PC399_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF2593242.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(14227..14739)
                     /locus_tag="PC399_002386"
     CDS             complement(14227..14739)
                     /locus_tag="PC399_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF2593243.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            15259..16194
                     /locus_tag="PC399_002387"
     CDS             15259..16194
                     /locus_tag="PC399_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF2593244.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(16286..18157)
                     /locus_tag="PC399_002388"
     CDS             complement(16286..18157)
                     /locus_tag="PC399_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF2593245.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            18347..19273
                     /locus_tag="PC399_002389"
     CDS             18347..19273
                     /locus_tag="PC399_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2593246.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            19412..19558
                     /locus_tag="PC399_002390"
     CDS             19412..19558
                     /locus_tag="PC399_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593247.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(19663..20187)
                     /locus_tag="PC399_002391"
     CDS             complement(19663..20187)
                     /locus_tag="PC399_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2593248.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(20240..20464)
                     /locus_tag="PC399_002392"
     CDS             complement(20240..20464)
                     /locus_tag="PC399_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593249.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            20661..21785
                     /locus_tag="PC399_002393"
     CDS             20661..21785
                     /locus_tag="PC399_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HDF2593250.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANSVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(22259..23083)
                     /locus_tag="PC399_002394"
     CDS             complement(22259..23083)
                     /locus_tag="PC399_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593251.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(23431..24486)
                     /gene="ispG"
                     /locus_tag="PC399_002395"
                     /gene_synonym="gcpE"
     CDS             complement(23431..24486)
                     /gene="ispG"
                     /locus_tag="PC399_002395"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF2593252.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(24668..25672)
                     /gene="rseP"
                     /locus_tag="PC399_002396"
     CDS             complement(24668..25672)
                     /gene="rseP"
                     /locus_tag="PC399_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF2593253.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(25685..26839)
                     /locus_tag="PC399_002397"
     CDS             complement(25685..26839)
                     /locus_tag="PC399_002397"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF2593254.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(27003..27206)
                     /locus_tag="PC399_002398"
     CDS             complement(27003..27206)
                     /locus_tag="PC399_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2593255.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKSI"
BASE COUNT         9142 a         4368 c         3169 g        10589 t
ORIGIN      
        1 tatttgataa atattcttaa aaacatattt ctataatatt tattttatcc ttagtatcta
       61 ttttttaaaa tctagttcta taacatctac tttatcctta tgtctattta taaaactaat
      121 ttgaaataga ctttctaaat ctaatacagt tatttttaga aagtctattt ccttaaataa
      181 actatttaat attactggct actttttata tgaatacaat aatttgttat tcataaataa
      241 aagcttattt catttcattc ttcatttgct tctctaattc tttagaaaat ttcttttgtt
      301 catatctttc catcatctca tcttttttca tttctaagtt taccatagca ccttgtgcat
      361 catctttcaa atcttcagct gtatttttca taacataagc tgtcttttta acaccttctt
      421 ttaaatcttc tttcatattt ttcatatctc tttttaatct atccatcaaa actacctcct
      481 ctgtaaatat aatacttcct atatttattt ttcccaaagt acatatattt atttgtaata
      541 tcaattataa tttttaagtc ttaaatatag tttaaactat ctaaatctat acatttcaat
      601 aaattaaata ttttttattt caaaataaaa ataacactaa gcatagatta tatgctcggt
      661 gttatttttt aaattcatag taacttaatt gttgggttac tataatgatg acttatatgg
      721 aggatgtaag gaaatctcat cattatagtt ggtgtatttt atggttttaa gcttctttat
      781 atttatataa tacagtttca ttatgaattt tttgtgattt taaaatgaac tattattgaa
      841 agttatatat tctttataat aagtaattaa tttttaaatt ctactgtgaa tactacagag
      901 ttttctttgt tttttatgct ataatcaaac ccatgacttt taaaaatttg gctgactata
      961 taaagtccaa gtccacttcc tccagttttt cttgaacgag atttttctat tctataaaat
     1021 ggattaaata tttcttctaa ataacgtttc tcgatagtta caccagtatt ttcaatttct
     1081 aaaaccactc tttgattaga agtcttttta cttattcgtt tatttttatc atagagtcta
     1141 attataagct ctgattcttc aggagaatat tttatggcat tatttattat attattgatt
     1201 gccttagtta ttctctcttg gtctgcttta atttccaaat tttcttctat ttttaaaata
     1261 gttttcatat gtttttcttc tattaaaaat acttgtctct ttacaagtct atttaataat
     1321 tcacttatat ttattgatac caatttcaaa tctttttcaa gtatttctga ttttgaaacc
     1381 tctatcattt catttactaa atcttttaat tcttgtgtac actcatacga ttttttcaga
     1441 taagtatccc tatccttata cttacctaca ctataaatca taccttcaat ttgaccactt
     1501 attatggtta ttggagtttt tagttcatgt gatattgtag caacaaaatc cttccttcta
     1561 tattcctgtt ctctctcact ctctattatg tttataagag gctttgttat tacaattgaa
     1621 tatatatatg ctccaataat cgctatcaat attgctattg atatgatgta tggcattagt
     1681 tttcttataa cttcgtttgc ttcgtcaatt ggttgtaatg gcataacgag ttctaaagta
     1741 tatggaccat cttttgcatc tttagtatat ataggtatag aagttctata ctcatcctca
     1801 agactattaa tcatgtactt actatatctt aaaataacta cttcattctt accatataca
     1861 attttacctt ggttatcttt taataaaata gccaaatttt ggtctttagc catataataa
     1921 agtctttctt ctaaagtata tgtatcgaaa tgaattgaac tatctactaa agatttcaaa
     1981 ctttcttgaa gagactcaat tttatactca tgataatatg atggcaataa aaaatataaa
     2041 attagatata ttattaaagc taatgctatt aataatgatg tcgttattga aaataactta
     2101 tactttatac ttaacttttc ccacttatca aatatccttc tcaagtgtat atcctattcc
     2161 ttttacagtt ttaatatatg gtaatgatat tttttttctt atatttttta tatgtgcatc
     2221 aacaatccta gtatctccat aatagtcata accccatata ctatccaaaa gaccttccct
     2281 agttataact tgtggatact tttctatcaa agcttttaat atattaaact cttttagtgt
     2341 aagttctata ggttctccat ctatctctgc aatatatgtg tttaaatcta attttaattt
     2401 ttcaaaaact ataaatttgt catttatagt cttgttactt cgccttaaaa ttgcttctac
     2461 tctttttata agaagattaa atgaaaatgg ttttgttata taatcatcac attctaagtc
     2521 aaacccttta agttggtctc cttcatcatt taaagcagtt aaaaatataa ttggaacgct
     2581 tgatgacttt cttatcattt tacaaactcc ataaccatct aagtttggca tcattatatc
     2641 taatattact aaatcataac ttccttgctt aaacttttgt ataccttcca acccatcatt
     2701 tgctgtatca acttgataac cttctgcact taaaaattct gatattaact cttgtatatt
     2761 tgaatcatct tcaattacaa gtattgacga attcattcta tcaatccttt ctattttatt
     2821 taaaatttcg tatattactt atattttatc ttattgttgt gaattttaca tgaaaaaaga
     2881 gctatcttaa tttgtaaatt aatcctttta atttaaagat agctcttttg gatacatttt
     2941 atttaattta ttaatctaat ctctcttata tgccttgaag tttttaagtc taagtgcatt
     3001 tgatacgact gaaactgaac ttaagctcat tgctgctgct gcaatcattg ggtttaataa
     3061 aggtcctcca aatacatata atattcctgc tgctacaggt ataccaattg tattatatcc
     3121 aaatgcccaa aataggtttt gttttatatt ttttatagtt tcatgactaa gtttaattgc
     3181 tgttggaaca tcaattaaat cactcttcat aagtactata tccgcagatt caatggctac
     3241 atccgttcca cttcctattg ctatccctat atctgctttt gcaagagctg gggcatcatt
     3301 tattccatcc cctaccattg caacaaactt tccttgattt tgtagttttt caacttcttt
     3361 tgacttatct tctggtaaaa cctctgccaa aaccatatct ataccgactt gatttgctat
     3421 agcatttgca gttttcgcat tatctccagt taccattgca acttttattc ccatatcatg
     3481 aagtatttct attgcttttt tactactttc tttgactaca tctgcaactg ctattatacc
     3541 tgataaattt ccatctacag ctatatacat aggtgtttta ccttggcttg ccaatatatt
     3601 tgatttttct tctaaatctc caagctttat attattgtca ttcataagct ttctatttcc
     3661 aagtaatata ctttcatcat taatagtaac ttgtatacca gcacctggta tagctttaaa
     3721 attatcaact ttttcaaatt ttatattctt ctcttctcca tacttaacta ttgcttctcc
     3781 aagaggatgt tctgaacctt tttctgcact tgaagcaatc ttgataagat attcttcttt
     3841 tacattatta tttaatacta tatctgttac ttttggcttt ccttctgtta tagtacctgt
     3901 cttatcaaat atcactgtat ttactttatg tgcagattct agtgcctctc caccttttat
     3961 caagattcca ttttctgcgc cctttccagt cccaaccatt atagcagttg gtgttgctaa
     4021 tcctaatgca caaggacaag caattacaag tactgatata aatatagtaa gtacaaatac
     4081 tatatctttt cctcctatta aaaaccacaa taatgatgca actacagcta ttgcaattac
     4141 tattggtaca aaatatccag atacagtatc ggcaagttta gcaataggtg cttttgtacc
     4201 ttgtgcatct tcaactaact ttattatttg ggcaagagca gtatctccac caattttttc
     4261 agccttaaac ttaataacac catttttatt aatactagct cctgtcacct tacttccaac
     4321 attcttttct actggtatac tttcacctgt aagcattgat tcatcaactg atgtatatcc
     4381 ttctattaca actccatcca cgggtatttt agttcctggt tttactaata gtatatctcc
     4441 tatctctact tcttctatag gagtttcaac ttctttacca tcaactaaca ctattgcagt
     4501 tttaggttgt agccccataa gctttttaat tgcttctgat gtttttcctt ttgattttga
     4561 ttctaaatat ttaccaagta agataagtgc tattattatt cctgcacttt cataatacaa
     4621 ctgatgatgg tgcatacctt gaatttgtcc attggctatt tgtagtgtcg tatatagact
     4681 atataaaaat gcggctaatg ttcctattgc aactaaagaa tccatattag ggcttaaaga
     4741 aaaaagtgat ttaaacccat ttatataaaa tttataacca gctatcatta ctggtattac
     4801 taaaattaac tgaattaatg cataattaaa agtatttgtc ataggattta taatttctgg
     4861 caaaggccat ggccctatag gctttattat cataggtccc attgctatat aaaacagagg
     4921 tactgcaaat actattgcaa ctataaattt aacaaataaa gacttcattt ctctttcttt
     4981 tctcaattta tcttcatcca catcaacctt atttcttacc tcttctattg gtttatatcc
     5041 agctttttca atagctgctt ttatttgaga taattttact tttgatgggt catagtctat
     5101 attagcttta tctgttgcaa tattcacact aatactttct actccatcca atttttttac
     5161 cactctctct acagcttttg cacaagctgc acatgtcatt ccatcaattt tcatatcaat
     5221 ttttttatta ctttcttcct ttataattcc atatcctgct ttttctacaa cctgctttat
     5281 atcatcaaaa ttaaccttag aattatcata ttctatttta agtttttcag tagctatatt
     5341 tacattttgg tcatatactc catccatctt cttagttact ctctctactg cctttgcaca
     5401 agctgcacat gtcatacctg taattttata actttctacc actttatttg aactcataat
     5461 aaaatcttcc tttctttcaa tatattttca gctaatttgt aacgttttat tttgatatgt
     5521 aatattgata aattacttat ttagaaatct atattttcat tataccccct ataggtatat
     5581 taaatcaata tttttttaat aaataaatgt attagttata tttatttgta tttaatagaa
     5641 aagtatactc ataattaaat gtatatataa aaatagagca tctaaattgt ttaatgctta
     5701 tacaatttag atgctctatt tattataatt tatattttat ttaaaattta ttctttacat
     5761 gcttcaaaaa actctgcttc tgtttccttt atttcaagct cattgtctat tattccaaca
     5821 gcttcttttt taaatgctct acactgacct atacaaccag tctttacatt ttcttctcca
     5881 accaaagttt ttagcttatt tacatccaca tttgaacagt ttggacatac tcttatcata
     5941 tttaagttcc tcctaattaa tgtctaatta atacttttat acccaaatat aaactttata
     6001 aaagtttttt taatataaat tattaccata tataccattt atatcctatt gcgagtatta
     6061 catattaatt ttatatatta ttaatactac atgtacggtg gtatctgttg ttttctcact
     6121 atttccaatt ccttaatttc taaagactta atcacattta ctttatagtt ttctttagac
     6181 tttatataca aaacatcttc tatcttttta ccttcaataa atttatgaga ccacttaaga
     6241 agttcttcaa aattacaaac ttcttctttt acaaaagtat cactttcact tgcaaaatat
     6301 ttaattatta ttttggttac ttccaacata tatcatcctt tactctttta ttaatctttt
     6361 tatctattaa ggttattatt tgtctaaaaa atttttttaa acagaataat tttcagatgc
     6421 aaaactaaca gttttatcca atttaaacta tattaataca atttaatata ttcctccttg
     6481 tacctaataa tatattgtgc tataattaat aagttatttt aatcgaaggg agcaacaaaa
     6541 atgtcacaaa aacataaaat tataaatatc gcagttatag cccacgttga cgctggaaaa
     6601 tcaacattgg ttgatgcttt cctttctcaa agcggtgttt ttagaaagaa cgaagtagtt
     6661 aaagactgcg ttatggacag taacgacctt gaaaaagaaa gaggcataac tatatattca
     6721 aaaaactgtg caataaacta tgaagattac aaaataaaca tagttgatac tccaggacac
     6781 agtgactttt cttctgaagt tgaacgtgtt atgaaaactg ttgacacagt tattctatta
     6841 gttgatgcta gtgaaggccc aatgcctcaa actagattcg ttttacaaaa atctttagag
     6901 ttcggtttaa aaccaatatt atttataaac aagattgata aaaaagacca gagagcggaa
     6961 gaagtagtta atgaagtttt cgatttattt gtagacttaa atgcaacaga tgagcaatgc
     7021 gaatttccta taatatatgg tattgctaaa caaggtattg ctaaacttga aatggatgat
     7081 gatagtgaag acctttctcc tctatttaaa actattgtaa atcacgttga agcttatcct
     7141 aattacgata atgaacctct acagttccaa atatcagctc ttgcatatga tgattatgta
     7201 ggtagacttg gtataggtag aatttacaaa ggtactctta aaaacaatac acaagttgct
     7261 atatgtagag aagattctgt tgtatctaaa ggaaaagtat caaaactttc tgtgtatgaa
     7321 ggtttaaaac aagttgaagt tgatgaagct actagtggag aaatagttgt tatagctggt
     7381 attcctgata tatctatagg tgaaactata tgcgatttag atagtccact acctatggaa
     7441 atgataaaaa tagaagagcc tactctttct atgaacttct tagtaaacga ctctccattc
     7501 gttggaaaaa gtggaaaatt cgttacaact agacatttga aagatagact tgaaaaagaa
     7561 cttgaagtaa atgttggtct taaagttgaa cctcttgata ctactgatgg atacaaagtt
     7621 tctggacgtg gagagcttca cttatctata ctacttgaaa atatgagacg tgaaggttat
     7681 gaagttggtg tttctaaacc tgaagtttta atgcataaag aagatggaaa actaatggaa
     7741 ccaatagaaa gagttgttgt aaactgtcct gaagtttact ctggtactat tataaatgaa
     7801 ttaaacatga gaaaaggtat gatggagtct atgtcaatag aaggagacta tgtaaaaata
     7861 gagttcttag cacctactcg tggtctttta ggatatcgaa gtgaatttat aaacgctact
     7921 cgtggagaag gtactttagt tcgttcattt gaaaaatttg aagaatttaa aggagaaata
     7981 ccttcaagag gaaatggtgt tttaatagca caaggacctg gtgttactat ggggtattct
     8041 cttaatgcct taagtgatag agctgtaatg tttgtagacc ctggtgtaga agtttacgaa
     8101 ggtatgataa ttggtatgaa ttccagaaaa gatgatatgg tagtcaatcc ttgtaaaaac
     8161 aagaaaatga gtaacgtacg tgcttctggt tcagatgatg ctataaaatt atctcctcct
     8221 agaatattta ctcttgaaga agctctagag tttatagaag atgatgaatt agttgaaatt
     8281 acacctgatt ctataagact tagaaaaaga ttcttaaatg aacatgatag attaagatat
     8341 aacaaatcta gacaaggtaa ataaaacgta catttacctc taattaacaa aatagccata
     8401 tagtactgaa aaattatttc agtttatatg gctattttta tttgaaattt atattattca
     8461 acatttatat aattagctgc ttgaacggct gcaatagctc catctgatgc tgctgttatt
     8521 atttgacgta aaaacttagt tctactatct cctgctacat atacaccttc tactgatgtt
     8581 gtacaagact catctgaaat tatatatcca ccttcatcca aatcaagaac attttcaaac
     8641 attttattat taggtttaag acctatagca ataaaaatac cactaacatc tatagttctt
     8701 ttttcttcag tctttaagtt ttttacttct attttcgata cagtcttttc tccttctatt
     8761 ttttctacac catgactgta aagtatctca atattctctc ttgctttcac agatttctcc
     8821 aacacttctt cacctctaaa actatctctt ctgtgaatta aatacacttt tgtacaatta
     8881 ttagctaaaa ataaagcatc ttctagtgca gtatttcctc cacctactat agcaacttct
     8941 ttatctttaa aaaaggctcc atcacatgtt gcacagtaag atactcctct acctgtaaat
     9001 tcttgttccc ctgcacaacc taatttacgt ctttcaactc catttgcaag aataacagcc
     9061 tttgctttat gttcacctga agaagttttt acaaccttta cttttccctc taaatttatc
     9121 tcttcaacct catcaaactg aatatcaaca ccttgagcaa ctgcttgatt gtatatatta
     9181 ttagaaaatt ctaccccaga aatcttttga actgctggat aattttctac ctcagaggta
     9241 cttgcaactt gacctccata aatatttttt tcaaatactg taacgcttaa ccctgctctc
     9301 atagcataaa ttgctgaagt taaacctgct ggtccagcac caattacaat gatatctacc
     9361 ataacgttta cctctcttta tattattatt tcttatatat tattctcatc attcttatac
     9421 tttactatta ttatccatat atactataaa caaaacatta ttttttatta attaacttta
     9481 ttttacatat atataaaaaa actgcaaata aaaattaact tttatttaca gttctcactt
     9541 atttatataa ttcaaaacct tattggttat catttacagt gtcttctaat atattgcttt
     9601 ctgatatata tgatagaatt gaaagtccat acataaccaa aactaatgct gctgttataa
     9661 gtactattcc catatttaat acctcctttt gtttacagta tactattatt atgtgaaata
     9721 tttatgaatt taatttgaat atatatgtaa tttcacacta acaatttctt caaattaaac
     9781 attattaact atatactttt gttgaaccaa ttatgattaa tagtttaaat attagcacta
     9841 tttacttata tttatatgat ataccatata attacatttt tcaaattaaa gttttgtaaa
     9901 ctttttttgt aatatagtgt agtataaaat tgtaatatta cagatattta atagttatct
     9961 tttgattact ttcattaaaa acagcttcat cactatgaaa atctaggttt ttaaactttt
    10021 aaaaaagtta aaaaaatatt tttctaatac tcaatttgat tacaattttt ttacaattta
    10081 aaaaatattt tttccaaatt tttatattaa tacttgactt tgtctgtttt ataaactaga
    10141 ataattaata atattaagaa tattgaaagg cattgattag gaagagtaaa tctgaatcga
    10201 ttaccagaga aggaaactac tagctgaaat gtttccctaa ttagaaggat tgaaaactac
    10261 ctaggagctt ctatccgaag ttatcttaat gattttagta ggaatagacg tttttattac
    10321 ttgcgttaaa gtatcaagtg gattttttaa aatcaatttg ggtggaaccg cgggaaacag
    10381 attctcgtcc cttttttagg gatgagggtc tttttttatt acctaaattc agtttttaaa
    10441 atattctgta tctaatttaa ttagctaatt aaaaatttaa aatatatgga ggattataaa
    10501 aaatgtatta ttcaagtacg agaggcaccg aagaaaaagt aactgcatca caagcaataa
    10561 taaaaggaat atcaaatgat ggtggtcttt atgttccaag tgaatttccc aatgtaaaaa
    10621 atgaacttat aaatttagta aatttaacgt attctcagat agcatttttt gtacttagta
    10681 aatttttatg tgattttact gaagatgaaa ttaaaaattg tatagaaaat gcatatgatg
    10741 aaaagtttga ttgtagtagt atagctccat taaataaagt aaatgatact tatttcttag
    10801 aactatatca cggtccaact ttagcattta aagatatggc acttactata atgcctcatc
    10861 ttcttaaaac atctataaag aaagataatt tagagaaaga tgttgtgatt cttactgcta
    10921 catctggaga tacaggtaaa gctgcacttg agggatttaa agatatagat aaaataaaga
    10981 taattgtatt cttcccagaa gatggtgtaa gcccagtaca aaaacttcaa atgaaaactc
    11041 aaactggaaa aaatacatat gtagttggta taaaaggaaa ttttgatgat gcacaatcag
    11101 gtgtaaagaa gattttttct gataaagaat ttaatcaaaa actattggat aataattata
    11161 tattatcttc tgcaaattca attaatatag gaagacttct tccacaagtt gcatactatt
    11221 tttattcata tatgaaatta gttagcaata gtgatataaa tttaaatgat aaaataaatt
    11281 ttgttgtccc aactggtaat tttggtaata ttctagctgg atattatgcc aaacaaatgg
    11341 gtcttccaat aaacaaatta atatgtgcat caaatgataa taatgttctt tatgatttct
    11401 ttaaaactgg tgtatatgat aaaaatagaa cattgaaatt aactacttct ccttctatgg
    11461 atatattaat ttcaagtaat ttagaacgat tactgtttga aatatcaaat agaaatactg
    11521 atattgtaaa taagctttta tcagacctta gtgataaagg agtctacaaa ataagtgatg
    11581 atatggaaaa aaatttagcc tcattctatg gagaatattc aaatgaagaa gaagttaaaa
    11641 gcacgataaa caatgtgttt aaaaattata attatttgat agatactcat actgctgttg
    11701 catacaattg ttatgaaaaa tataaaaaag aaacttctga taacacaaaa actgttattg
    11761 taagtactgc aagtccattc aagttttctg aagatgttct aaagtctata gattgtgatt
    11821 ttaaagacct tgatgacttt gctataattg atagattatc aagtatttct aaaatagata
    11881 ttccaaaacc tattaaaaat cttaaaaatg cagaaatact acataaagat atttatgaaa
    11941 aagatgaatt aaaactagct ataaaaaaat tcttaaaggt ataggtgatt attatgttag
    12001 agattatagt tcctgcaaca agtgctaata taggtccagg atttgattgt cttggaattg
    12061 cgttaaatat atacaataaa ttttatgttg aagaaattga aagtggtctt gaaatagaag
    12121 gttgtgaaga tgcttacaaa aacgaaaaca acttagttta tacatctatg aagtactttt
    12181 ttgatagagt taaaccagaa aaaatacctg ctggtatcaa aataaaaatc caaagtgaag
    12241 tacctatatg tagaggtctt ggcagtagtg cctcttgtat agtagctgga gttatagctg
    12301 caaatgctct atcaggtgct aatttagata aaaatcaatt actaaatata gcttcagaaa
    12361 tagaaggtca cccagataat gttgctcctg ctattttagg aaatatgatt gtatctgtta
    12421 ctgataatga gaatatacat tatgatatta taaaaattcc tgaagaactt aaattttgtg
    12481 caatgatacc taattttaag ttatcaacag aaaaagctag aggtgtttta ccaaaggaaa
    12541 taccatattc tgatggggta ttcaatgtta gtagagttgc tttactaata tctgccttac
    12601 taaacaaaaa ctttgaccta cttaaagtag cttgtcaaga caaattgcat caagattata
    12661 gaggaactct aattgagaac tacaatgata ttgttgaaaa atctgaacaa ttaaatagta
    12721 ttggagtatt tttaagtgga gctggaccta ctataatgtc tttaataaaa gaaaatgatg
    12781 atagttttgt ggataatatg aaaaattacc tacaaaaact aaaatctgat tgggaaataa
    12841 aagagttatg ttgcgattct aatggagctg ttttaaatat aatataatta tcgtcttcta
    12901 tacaaaatat aactactctt ttattataat ttgtagtata ataattagga ctaggtaata
    12961 atattaagta ttataatcta gttctttaaa catgtttaca ttgaggaggg attatcataa
    13021 aagagttaat aattagagat ttagttgcaa aagttcctat aattcaaggt ggaatgggcg
    13081 ttggtatatc taggtcatca ctagctggtg cagttgctaa gcttggtggt gttggtgtaa
    13141 tttctggagt tcaaattgga tatgatgagg aagattttga aactaatact ataaatgcaa
    13201 atttaagagc tataaaaaaa catatttcta aggccaaaga aatctcaaat ggtggtatta
    13261 ttggtattaa ttttatggta gctatgaaag aatatgaaac atacgtaaag gaagctgtta
    13321 aagctggtgt agaccttata atatctggag ctggattacc taataagcta ccatcattag
    13381 taaagggaag taatgtaaaa atagcaccca tagtttcaac tgcaaaagct gctaatgtaa
    13441 ttttaaaaat gtgggataga aaagagaaaa ctactgctga cttaatagtt gttgaaggtc
    13501 caaaagctgg tggtcacctt ggatactcta acgaggaact tgataacata gattctatag
    13561 actatgataa ggaatttgtt gaaattttaa aggttgcaaa tacttatgga gaaaaatttg
    13621 gtagaaacat accagtagtt gctgctggtg gtataacttc tagtagtgat gttaaaaaat
    13681 atatagatat gggtgcaagt ggtgtacaag ttggtacaag atttgttgct acttacgaat
    13741 gtgatgccca tgagaacttt aaaatggcat acataaatgc tagtgaagaa gatgttcata
    13801 tagttaaaag tcctgtaggt cttcctggac gtgccataag aaacaaattt atagaagaag
    13861 ttaaaataaa tcgtccagaa ataaagaagt gctataattg tttgattcct tgtaatccaa
    13921 aagaaactcc ttactgtatt tctcaagctc taatcaatgc agttaaagga gatgttgaaa
    13981 attcactttt attttgtgga aatgatgcct ataaaattga taaattaagt actgttgaag
    14041 atgttatcaa tgaattgatt tctgaattat aatttaaaga aaactaaatt aaaattttat
    14101 caagctaagg agtttaaata cgttatttat taatctttat aaacatattt atgtccttag
    14161 cttttttgtg tcaatttcct tttattgaaa ttttaattta attagagata gtattaaccc
    14221 ttataactaa tcttctttta caagtacatt ggttatttca gctatatata aagtatgata
    14281 atctttttgt ggataccatc ttccatctat accttcagca ataaaacatt ggggttcaag
    14341 tttctgacta tacatttttt tgcatataat agccatttta gcttccttaa aataaggtgt
    14401 atcatttaag tgttctatat taaatccaat ctgtgatatc ttatcctctt ctctaccaga
    14461 tacttttcca caatatgaaa gctctttacg aaaatcttca tcaaaaaatg taagagaaaa
    14521 tttatctgta ttatctataa attccttagt ataacgctgt ggtcttatta ctacaaaagc
    14581 aacatctttt ccccacataa ctccaagccc tccccagcta gcagtcattg tattcacttt
    14641 accttcattt tctgctgtta taagtgtcca atctttacct atcaatccaa atggattatt
    14701 tttaatctct tctgccaaaa tttctttaaa atcactcata tctctaataa cctctcattc
    14761 tcttatattt atattccttt tattttcatg ataaattata aatgaagtct agttttgaaa
    14821 ataaaatact tttttaaata tattattgca aataagtaaa tatatatata cactcttatt
    14881 ttttaatata tttaataatt tcactcagaa tatatatatt ctttactcaa attaaatata
    14941 acttatatac tttatgtgta aattatggta aaaatattat ttaatcaaat caaaacttga
    15001 tttatatacc tggaaaaggt attatattaa agagtgctta aaatacgcat ttcataaata
    15061 gctatatata aaataataaa taaggagagt gatgggcaat ggatactagt ccgttgcgga
    15121 gagaattttt tacattttca tcacttaata atatctaaaa tcaaaactaa tacatatatt
    15181 cagttatata aattagttaa ttaatagata ctattaaatg atgagattta tataaaactg
    15241 aataaaggat gtgttgccat gataagatat tacaaaacta tagattcaaa attagagaaa
    15301 cttagttttt ttgaagatgg ctgttggata aatcttgttg aacctaatca tagtgaaatc
    15361 aacgaaattt ctaatttatt aaatatagat gtagaaagca tagaatctgc actagatgaa
    15421 gaagaacgct caagaataga tgttgaagac aatcatactc ttatacttat agatatacca
    15481 gtagacgaaa gtgactctaa ttcttctcat tatactacaa tacccttagg tattatacta
    15541 actgaagaag ctattgtaac agtttgtgat gcacaaacta agattttaaa tgactttata
    15601 gttggtcata ttaaagattt ttttacgttt aaaaagactc gctttttgct tcaaattctt
    15661 cacaaaaatg cagcttatta cttgcattac ttaagaaaaa taaataaaat gacaatcata
    15721 atagagcgag aaatttacaa atcaatgaaa aacaaagaat tggttcaatt attggaatta
    15781 gaaaaatctt tggtatactt ctctacctca ctaaaatcta atgaacttgt tttaaacaaa
    15841 atggttagaa ctgctggtat aaagaagtat ccagatgatg aagacctatt agaagatgtt
    15901 atagttgaaa atagacaggc tcttgatatg gctaaaatat atggagatat attgagtaga
    15961 attatggatg ctttcagtgc aattataagt aataatcaga ataatgttat gcaaatttta
    16021 acagtagtta cactaatttt ctcaatacct acaataatat caggattctt tggtatgaat
    16081 gtcatcaaca tgccattttc taataaccca aacggatttt ggataatact tcttattacc
    16141 tctataatat gtatagtgat tacatttttc atgtcaagaa ataagctatt ataaaatatt
    16201 aataaaaaag ccattagaat taattgattt acatttatcc taatggcttt tatatattta
    16261 aatctatata atcttataat actttttaac tatcatgttc ttcatctata atcatttcag
    16321 tttcaagccc ttctagcatt gaatctaaac tttcttttat atttagtata taaattggta
    16381 catactcttc ttcatctatt acctcttctt gcaacttaaa tggaggaatt aatacttcat
    16441 caaagtctat atttaaattt ggaaagcctt ctactatctc ttcacaatta tcgtactctt
    16501 catttgctaa agaaatgtac atatctaggc tctttttata ataaagacct gcaagcatat
    16561 actcacactt cttacttcca tatttaatac cttctttata atatttctca gcttctgcgt
    16621 catcatttaa ttttaaataa atttgaccta aattatatat tgattcaaca caaccttgtg
    16681 ctctagcctt ttcataccaa aatttagaat ttacataatc tttatagaac atatcataaa
    16741 ttaatcctac catatgttga gcatacacat tattttcatt ggcaggtgta tcaaacatca
    16801 ttttagactc ttccaattta ccttctctaa aataaattct acctagatgt atacgagcat
    16861 tcatatggtt tttgctactt gccatttcaa aatactttct agcatttttt gtatttccta
    16921 agctctcata tatacacccc aatctataac tagacctcgt atctccattt tcagatgctt
    16981 tcttatacca acttattgct tcttcaatat tatttaaatc ttcatagata tttcctaatt
    17041 tgatttgaca agatacatta tttggaatcc tcgaataata cgaaatcgct ttttctatat
    17101 cttgattctg ataatataaa tcccctagat tttcaaggga acttttgcat ccaacagcta
    17161 tagcatcctc ataatattta atagcatttt cataatcttt ttcttcaaaa tatatacctg
    17221 ccagattatt ttttgcttct gttatatcat cttcagcagc cattttataa taagtttttg
    17281 ccttctctat ctcattttct ctattatata aattagcaag tctatactta gaaagtgtat
    17341 gatttttttc tattgctttc tcataataat aaattgcatc tcccagctct tcttttctat
    17401 catatacata tgcaagccta tactcagact tcacatcatc tctatcagca ctctttttat
    17461 aatacttctc tgcatcttct aaattatcat ttatttccat aatcataccc aagttaaagc
    17521 aacctttcat atgacctgac ttgtacaact gtttatataa acttactgtt gatttattat
    17581 ctttcttttt tccatttaat attgctaaat tatattttgc atcatcatct aaaagcacag
    17641 aacctttttt atagtattca tatgcttcat catctttctc caaaaaagta tataataccc
    17701 ctaaattaaa acaagcttta cctaaattct tgtctgctgc tgtttttaac catttttcac
    17761 accttacaaa atctttcatt ttaaaatggc atacacctaa attattttgt gcttgtaaat
    17821 aacctaaatt tgctatcttt tcataccaat atatagcata ttcatagcca tctaacttat
    17881 agtacatatt acctaaatca aaagcagcat cttcacttcc acttttatat gccattgaat
    17941 accacttttc tgcttcttta aattgttttt tactttcata tatatttcct aaagtatatt
    18001 gagattttac gtaattatcc aatgcagcac tcttatacaa ctctattgcg ctttccatct
    18061 tacccatctt ttcaaggagg gcacccttag tatgtacgac tttcgggtca ttagatatat
    18121 tttttatttt ttttatagaa aattttgtat ttttcattat tcgctaccta cccattttat
    18181 aaaatatatt ctctcaaaca ttattataca ttataatcta caattaatgc tataatattg
    18241 aatttttacc ttttgtcact atattgtaac attaaatttt aaattttata atcattagat
    18301 tttattatgt aattggtata taatgtatat atagggggga cattttatga atttcagcaa
    18361 tatttttatc caagttgcag ttttatttat aattatttta gttggatatt ttgtcagaaa
    18421 attcaactta ttagatgacc actgcacatc taagctttca actttgacaa tgacagtttt
    18481 tttaccatca atgataatta gctccatgca aattaacttt gatagtaaaa tgatacaaaa
    18541 aatactattg ttattattta catctttgat tatgtatata gtttcaatta taattgcatt
    18601 tttattgaaa tacattttaa aatgtaatga taaaaaagat ttgggaatat accagtatat
    18661 agttgttttt tcaaatgttg cttttatggg ttatcctgta attgaagcag ttttaggtca
    18721 tgaagcaata ttctatactg caatattcaa tttgcctttt aacttatttt ctttcacttt
    18781 aggaatttat cttttatcta aaggcagtac aagtagaggt ttttcaataa aatcacttat
    18841 aagccctgct acaatagcta ttgttattgg acttttttta tttgtaacag gacttaggtt
    18901 accccaattt ataaacgagc cacttgagat gttgggaagt ataactacac caatatccat
    18961 gattatcatt ggaagcttac ttgctaattc ttcagctctc gattgttttg tcaacaaaaa
    19021 attatatata gtcactttta taagattatt ggtacttcct gtgattgtgt attttattct
    19081 aaaaggatgg ataaatgata agatgatttt agctatacca gtagtaattt cttcaatgcc
    19141 tgctgctgca aatactgcta taatggctaa tcaatatgac tccaatataa cactagcttc
    19201 acaatgtgtg ttttttacaa cactattttc agttatatct atacctttta taagtatttt
    19261 actcttatct tgatttattt ttttatgtat tatataataa catattcaat aaattacaaa
    19321 tataaacatt ttattgtagt atattgatgg aattttttcc tagatatttg tcaataatat
    19381 ctatatgtat tatagaatag gaggcttaaa tatggatatg gatttactct ttaaaatagt
    19441 atttgctata gttgctatag gtgtttctgt aaaaatcatt aaaactgttg ctagtttagc
    19501 ttttaaaata gcgttgatat tgctaataat tttatttgta tatagaatgt ttttgtaaac
    19561 atttaaaaat aagtttttta acacaataga gaagtaaaat agattatctt taatgtaaat
    19621 tttttattaa agacactcta ttttacttct ctattagctt atttaaatta aatttttatt
    19681 atatcttccc ataatataaa catcttcaaa ttttccatct ctgattacag catatttttt
    19741 agttccctct atttcaaaac ctagcttttt atataaattt aatccctttt cattatccac
    19801 aattacttct aatcctactc tagttaacat aatccaatta tctgctatat ctaaaagttg
    19861 ttccataagc ttcttaccta ctcctcttcc ctgataattc tctgcaacac ttattcctat
    19921 ttctgcgcta tgtctcaatc gtggagaaga atttacatgt aacccagcaa aacctattac
    19981 atctttttct ccattattat tagtttcagc tacaaacata taatcatttt ctcccaaaga
    20041 agctaacata gcttcagttt tatcgactct gtcactcatt aatgccaaag tatttctcat
    20101 aacactgtat gttcttctaa tttcattaaa agcttgtgca tcttctcttc ttataggtct
    20161 tattgttata tctataggct tactcatatt ggattccccc tcaaacataa tctttatttt
    20221 taattaaaat tttacatctt tattttatac tgttcaaatc tatgtatgct tctacctgag
    20281 gtacatatat catattatct tttatatgtg tacttccaaa ttcttttaat tctgtacctt
    20341 ctttatcctt agttatttta tatctaatgg attccatacc ttcaactact tcagcatgaa
    20401 aaaacatacc ttcatattgt ttaaatctaa aattaaaatt gtctttagta agttttatta
    20461 tcatattttt atctactcct tatataattg atatttttct tattattagt tgtatatata
    20521 ctgcttattc atattaatat attataactt atcatttatt tagtataaag ttataaatag
    20581 atatatttat acttatttta caatctttct tcaattgata tataatatgt ttatataaac
    20641 taatgtatgt gaggtgaaat atgagcaaaa aaacaaaata tttttcttta attctctttc
    20701 ctattgcttt gcttttaaat tttatagcca gcaaagttcc aaatattgtt gaaaaatatt
    20761 actcccaatt catagataaa ataattgtac aaatattgag taaagtttct ggaattttcc
    20821 cattttctct ctatgagatt acaatgtaca taatagttat atctatcttc ttatttttat
    20881 gttgtacttt atctacaatt tttaataaga aaaaaaaatt aaacatatat ttaaaaaatt
    20941 ccattttaaa tatattatcc attgtatcaa tagtctactt tctttttgta gtgctttggg
    21001 gaataaacta taataggatt ccactagaaa ccacacttat aaataactat aatttaaaaa
    21061 acaatacctc tatccaatca aaacaatata gtgttaaaga gcttactaaa ttatataaat
    21121 tccttgtaat taaagcaaat gaaactagaa aactcacatt acaagataaa aatggtattg
    21181 taaaatctaa tacagattat aatggtgtca taaatagagc tcaactagga tacgataata
    21241 tcttggatat attacctggt gttagtggta gttattctaa gcctaagtat attatttcat
    21301 caaatctaat gtgctataca ggaataactg gtatatactt tccttttaca ggtgaggcaa
    21361 atgtaaatat agcaatacct gacttatata ttccttgtac agttgaacat gaaatggcac
    21421 atcaaagggg atttgctagt gaagatgagg ctaattttat agcgtatctt acttctataa
    21481 aacaccccaa tattgatttt aactattctg gatatatttt agctttaaat tatactgctt
    21541 ctgctttaag taaggttgat tataatgcat atgttgatat ttctgctggc atttcagatt
    21601 ctgtccgtag agatttaaaa aatgaaagtg aattttggca aaaatatgaa ggtaaaatta
    21661 atgaaatttc taatgagttt aacaattctt acttaaaagc taatagcgtt actgaaggta
    21721 ctcagagtta tggtaaaatg gttgatttat tgctaactta ttatgaatta tatccatata
    21781 actaaacact acaattcaaa attttattca tatacagctg atattaaatc agctgttttc
    21841 tatttaaata aattatttaa cttcaagcaa tcttatataa tttgatagta cttatactat
    21901 ctgtataaca tttcaattct aactatttta gttaaaacat ttctattatc ttttttaata
    21961 aatgttttaa ctaaaatttt taaaacataa aacttccaca ttatttcact atctatataa
    22021 aaatctaaaa attcacatat aaaattacat aactaaaaaa tcataaattt atagaaaaca
    22081 tatattcaaa ataaaaaagc atgtatcaaa acttgacaca tgcttataat cttatgtata
    22141 tatatcattt tgtcacaata aaaatttact tgtaacactg atatttttaa acaaaatagt
    22201 ccttatttaa ttaaattaac attaattaaa ttcgtactca ttttgctatc attaaacatt
    22261 acttatagta attttctaat acactatttg aaacttcctt cccttgaaaa cttttcaacc
    22321 aattctcata tgctgagttt ttccatacac taccatcatt aaatgttact tgttttacac
    22381 aagatataat gtactttaca ccatgctctc cagttgattg tttccattta tcaaacaata
    22441 gaaaggcatc attttgattc ttacctggtg ctattgcatt ttgctttgca tattctttct
    22501 ccatttgttt ccattctttc aaccaatttt ctacccatgc cttgccttgt tgtttttcaa
    22561 acatacttat tatatcctgt ggtggtgatt gttgcatttt tctataagta tgtgaatatg
    22621 attttggaga cacaggagat atgcctgtaa caataccata gtctacacca gctgggctaa
    22681 atccaacact tcctacttcg ccatctgctg ccacattttg agcatcccaa tatagctcaa
    22741 gtggttttcc atttttatca tatgctaagc atgtaatttc ataatccata attgcctttt
    22801 cactgctatt tgttaagctt ataacattgc taggccaatg atatggagta tcatcatact
    22861 gtattttgct attatttata aataatggaa tattctcatt ataaacaaga cctttactac
    22921 tttgaatatc tgataccttc tttgtgacag tacttaccgt atttgctaag tatacatttg
    22981 ttttttctga attggtcgta ctaagagaaa atgttgttgt cgttccagta atcagcaaag
    23041 caacaactaa aataggtaag ctagatattt ttttatatct cacaattctt cacctctttt
    23101 tctattaact tataataaaa caatttacca aaacattaaa atctatgtta acataattta
    23161 ccaactgttc tagtaactat atcctatcat agttactatc aagtttcaat actattgatg
    23221 gaaattttca ctatattaaa tcttttttta attttataaa tttacaatat gatttaactc
    23281 atacatatat agtcaaatta gaatataagt ttcttacatt taatgaagtt tataaaataa
    23341 aaaatgacta gataattaaa tactgtaaat aaaaacttat tacaaaagta tttaaaaatc
    23401 tagtcaatta atatatatat gaattgtata ctataatttt tcaatctctt ctagcaactc
    23461 atcaactaat ttgttaccat caatcattct aactatttca ccctttttaa ataaaagacc
    23521 ttcaccttta cccccagcaa tacctatatc tgcctctctg gcttcaccag gtccatttac
    23581 agcacagccc ataattgcaa cagtgatatt tttttccata ctaccaatct tttcttcaac
    23641 ctcatttaca acactaatta agtctatatt acatctacca catgtagggc atgatataac
    23701 tttaatctta tcatttaaaa gtcccaaact tcttaaaatc tgtttaccaa caataacctc
    23761 ttcaacaggg tcgcctgtta aagatattct aacagtatct ccaattcctt taagaagaag
    23821 tgctcctacc cctatagaag actttattgt tcctttgtga acacttccag attctgtaat
    23881 tccaatatga agaggataat caactttttt tgatattaat tcatatgcat caacagtttt
    23941 ataaatatcg gatgatttta gagatataac tatattatga aaatctaagt cttcaagaat
    24001 cttaatatgt cccattgcac tttcaactaa tgcttcagca gttgcagagc catacttttt
    24061 taaaagttct ttctctaaag aacctccatt aactccaatt ctaattttta aatttctttc
    24121 cttgcatttc tcaactacca tcttaactct ctctatactt ccaatattac caggatttat
    24181 tcttattcca tccacacctt gttctatagc ttcaagggct agcttataat cgaaatgtat
    24241 atctgcaata attggtatat ttacactaga ctttatcttt gctatatttt tagcagcttt
    24301 aatatcagga acagcaactc taactatatc acaaccagct tcctctaatc tctttatttg
    24361 agctattgta gcatctgcat ctcttgtatc agtatttgtc atagattgta tacttatagg
    24421 attttctcct cctattttta cagttcctac acttacttct ctcgtctttc ttctctcgta
    24481 actcatattc cacctctata tattatttta tattgaatct ctttaaatat taaaatatta
    24541 taacctacat gtatattata aattaaaaag ctatctagtt aaataagaat aaatttcata
    24601 tataaaaatt aatttattct tatttaaaac tacatagcct attttcttaa aatctctaaa
    24661 aactaaatca ttgaaataat cttagtatat ctttatatgt gataaaaagc ataaataaca
    24721 ttaaagctcc aaaaccaaca acatttatca tgccttcttt atttgggtcg agttttttac
    24781 cacctctaac agcttctagt aacagcataa gtattctcca tccatcaagt gctggtattg
    24841 gtaatagatt tactatacct aaatttaagc ttatgacagc tgctaaatac actacattta
    24901 tgatgcctgt acgagcagca tctgatacca taccaataac acctactggt cctgctacag
    24961 cattgccaat ccctcctggt acacgtcctg taatcatttg acctagaaat gttagcatct
    25021 gttttgtcat atctacagta gttttacatg catttactat tgaccccaaa aagtcttttt
    25081 ctctttgtgg ttgaatccct atttcatact ttccattgtt atttttaggt gttacattta
    25141 caactttatc acttccatct ctagttatac taagttttaa ttctccacca gaagaagtat
    25201 ttattatatt tgcaacttca tcccaagtct taacagaatt accattaatt ttatttatct
    25261 tatctcctac ttgaagccct acagcctgtg caggagtatc tggcattatc tttcctaatt
    25321 tagtcgttgg agaacctata tacaaaaata ctggaactaa aagtattgct gctaatatta
    25381 tattgaatat tggtcctgct acaatagtac taaatctttg taatatagac ttgtttccaa
    25441 aagaatttgg acttatttgt tcttcatctt caccttccat gctgacatat ccaccaattg
    25501 gaagtgctct tatagaatac tctgtatctt tttttacact atatatctta ggccccatac
    25561 ctatagaaaa ctcatttact ttgataccac ttctttttgc aaatataaaa tgtcctaact
    25621 catgtattaa tacaattata ctaaatagta ttaatgcggc tattatagtc ataaattcac
    25681 cttcctattt ttttatcaga tttgcaacat aagctctact ccatctatct gtttctaaga
    25741 tttcttctaa tgttggttca cttatgctac tatgaacttc taaagtcttc tctatgtaat
    25801 atggtatgtc atagaaacca attttatcct ctaaaaattc acttactaaa acttcatttg
    25861 ctgaatttaa aacagaggaa taagttcctc ccatttttaa acactcatat gccaatttta
    25921 aacatggaaa agtttctaaa tctggttctt caaaagttaa agtactaaat tttgaaaaat
    25981 tcattctttc aaagctactt tccattctat ctggatatgt aagagcatac tgtattggta
    26041 gtctcatatc tggacatcct aattgagcaa ttattgagct atctgtatac tgcaccattg
    26101 aatgaattat gctttgtggg tgaactacta catctatatt ttcttgttct actccaaata
    26161 accatctagc ttctatcact tcaagtcctt tattcataag cgttgaagaa tcaatactta
    26221 tttttcttcc catactccaa tttggatgct tcaaagcttc atttttagtt atatttgcaa
    26281 gttctccctt tttctttcct ctaaatggac caccagatgc tgtaagtatt atcttttcta
    26341 tatttttttt attttctcca tttagacatt gaaatatagc actatgttca ctatcaacag
    26401 gaagaatatt tacattgtat ttttctgctt ctttcattac aagttcacca gcagttacca
    26461 acgtttcttt attcgctaat gctatgtcta ttcccttctt tatagcacaa agagttggga
    26521 ctaatcctat catacctact acagcagtta aaaggacatc cacttcatca agtgatgata
    26581 ttatttttaa tccttccatt cctgctaaaa cttctatttc tatattttta ggcaacattt
    26641 cttttaattt caatgcttta ttttcctcaa aaactgttac atatttaggt ttaaactcta
    26701 ctatctgttc tagcaataat tcaatattac tgttagctga tattgcaact atctcaaact
    26761 tatctctatt atctctaact acatccaaag tttgtttacc tatagagcct gtagaaccta
    26821 aaattgatat ctttttcata taataatcct ttctaataat aaatataccc tgtcaaaaca
    26881 ttataacact actaaaacaa ttatttttac tattcataga aatttcatat attattccac
    26941 agattaaaat taatctatta ttttttgtat tttgtggaat attataaaat attctagtat
    27001 tattaaatgc tcttttttat ttccacaaca tcttctattt tacagtctag tgctaaacat
    27061 atcttctcca ttgtatccat agaaacagat ttatcatttc ctagtctgcc taaaacagaa
    27121 atacttatac ctgatatatt tacaaattcc attttcttta tttttctgtc aattaacaat
    27181 ttccataatg gagcgtatga aatcatgtta tcacctcgca aaatatattc tatcacattt
    27241 tatctatttt ggaaaatttt catctatt
//
LOCUS       DAMJWS010000056        26764 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMJWS010000056 DAMJWS010000000
VERSION     DAMJWS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.056"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(164..1378)
                     /gene="dpaL"
                     /locus_tag="PC399_002399"
     CDS             complement(164..1378)
                     /gene="dpaL"
                     /locus_tag="PC399_002399"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2593256.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(1992..2529)
                     /locus_tag="PC399_002400"
                     /pseudo
     CDS             complement(1992..2529)
                     /locus_tag="PC399_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(2606..4411)
                     /locus_tag="PC399_002401"
     CDS             complement(2606..4411)
                     /locus_tag="PC399_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2593257.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(4807..5184)
                     /locus_tag="PC399_002402"
     CDS             complement(4807..5184)
                     /locus_tag="PC399_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2593258.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6089..6574
                     /locus_tag="PC399_002403"
     CDS             6089..6574
                     /locus_tag="PC399_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593259.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(6774..7313)
                     /locus_tag="PC399_002404"
     CDS             complement(6774..7313)
                     /locus_tag="PC399_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2593260.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(7306..7719)
                     /locus_tag="PC399_002405"
     CDS             complement(7306..7719)
                     /locus_tag="PC399_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2593261.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(7755..8876)
                     /locus_tag="PC399_002406"
     CDS             complement(7755..8876)
                     /locus_tag="PC399_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2593262.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            9475..11127
                     /locus_tag="PC399_002407"
     CDS             9475..11127
                     /locus_tag="PC399_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF2593263.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            complement(11510..12436)
                     /locus_tag="PC399_002408"
     CDS             complement(11510..12436)
                     /locus_tag="PC399_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2593264.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            13026..13946
                     /locus_tag="PC399_002409"
     CDS             13026..13946
                     /locus_tag="PC399_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2593265.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            14032..16452
                     /locus_tag="PC399_002410"
     CDS             14032..16452
                     /locus_tag="PC399_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2593266.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            16452..17474
                     /locus_tag="PC399_002411"
     CDS             16452..17474
                     /locus_tag="PC399_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF2593267.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            17627..18373
                     /locus_tag="PC399_002412"
     CDS             17627..18373
                     /locus_tag="PC399_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2593268.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            18459..19709
                     /gene="cme"
                     /locus_tag="PC399_002413"
     CDS             18459..19709
                     /gene="cme"
                     /locus_tag="PC399_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF2593269.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(20226..21239)
                     /locus_tag="PC399_002414"
     CDS             complement(20226..21239)
                     /locus_tag="PC399_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593270.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(21242..22270)
                     /locus_tag="PC399_002415"
     CDS             complement(21242..22270)
                     /locus_tag="PC399_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593271.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            complement(22270..23115)
                     /locus_tag="PC399_002416"
     CDS             complement(22270..23115)
                     /locus_tag="PC399_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593272.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            complement(23546..24676)
                     /locus_tag="PC399_002417"
     CDS             complement(23546..24676)
                     /locus_tag="PC399_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2593273.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            complement(24673..25344)
                     /locus_tag="PC399_002418"
     CDS             complement(24673..25344)
                     /locus_tag="PC399_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593274.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            complement(25547..26197)
                     /locus_tag="PC399_002419"
     CDS             complement(25547..26197)
                     /locus_tag="PC399_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2593275.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
BASE COUNT         9348 a         4291 c         3232 g         9893 t
ORIGIN      
        1 tatttagttt ttattttgaa tttttcattt gctttacttt ttcatttatt tagatttttc
       61 atttgtttta gtttttattt tatttaaagt tttcacttat ttaagttttt attttcctta
      121 aatttttcat ttatttgtta agtttttatt ttccttaagt cttttattta ctttggcatt
      181 caccattcca aactatatct ctatatttat cagggtcagt atctccttct gtacttatta
      241 ataatacttt agagttctca tctagtttca aagcttctct taagtctttt aaatcttcac
      301 tttcaaggat tgaaactaat gctccagttg taactgctcc agattctcca gagattactt
      361 tctcatctcc tcttagaggg tttcctaata ttctcatacc tttagcagat acccagtctg
      421 gtgctgaaac gaatgctgat gaataatttt ttaacacttc aaaacctata gtgtttggtt
      481 ctccacaagc aagccctgcc attatagttg gcatatctcc tccaacaaat cttggttttc
      541 catctccagc ttctgctgat ttatagtaac aatcagcctc atcagcctct actactactg
      601 ttattgggca ttcatcatat attgaagcaa catacccttg aactgcacca gcaagtgaac
      661 caactcctgc ttgaacaaat acgtgtgttg gtctatctac tccatattct tttaattgtt
      721 ctatagcttc tttagccata gttccatatc cttgcattat ccatgctggt atttcttcat
      781 atccgtccca tgctgtatct tgaaccatta ctccatggtc atcagcttct gcataatcat
      841 tagctaatct tactgcatca tcatagttta agtctgttat actagcatct gcaccttctc
      901 ctttgatttt atttaatcta aattcagaag aaccttttgg cattaataca acagattttt
      961 gacctaattt attagctgtc caagcaactc ctctaccgtg atttccatct gtagctgtaa
     1021 agaaagttat ttctcctaat ttttctttta tttctttcga agtcaatttg tcatacccta
     1081 actcagatat atctgtattt aatctttgag ctaagtatct acccatagag taagaaccac
     1141 ctaaaacttt aaatgcgttt aagccaaatc tataagattc gtctttaaca tatattccgc
     1201 ttacgcctag tgattttgct aagttatcta atttaactaa aggagtcttt tcatattgtg
     1261 gaaagctttc atgaaaagct ttagcctttg caatctcttc atcttttaaa aattcaattc
     1321 ctttttcttt ttcctctgat ttaggtaagt tatttaattt ccattttatt tctctcattt
     1381 attttatatt cccctttcaa atttttcggt gtcattttaa ttttttatct ttgtgcctta
     1441 tagcaaagca attaccgtgc caatattatt tattctaaaa acaatttttt tatgttggaa
     1501 ttacatactt ttataaaatt tctttaaagt ttataaattt tgagtgtgaa aaataagatt
     1561 tataaaagaa aaaattctca tattgggaaa tttttatcaa tacgagaatt atcaattcga
     1621 gaaatcgttt atttatctta attcttcctt tattatgctc ttatttttta tatgtctatt
     1681 gttttgtcag atatattact acttttattt tctataaata tatggctata accttcttac
     1741 gcataaactc tataaattta tatactttat tacccaaata ttctttatta cccaaagcct
     1801 ccaaaatggc ttaaatcttt tcatagcttc ttacagaagt ttacatggtc gtccccccaa
     1861 taagcaaaag tgcaatatgt gtaccattgt gccctacttg cgactattga gctgtcgaaa
     1921 ttcgcaactt cgcatttatc tgatgaaatg cactatacaa aatattcata acatccacaa
     1981 cagagttaaa gttacctatt catattcact gtaaaaattt cttccacagg aaatgtaatt
     2041 ttaaccaatc ctattgagac aaactcacca cttatttttt tgaactcacg cagtaataca
     2101 tcacttgccc gaaaaaaagc ctcactttca tcatctccag taagtgctat tgtgcaatgg
     2161 ggtacccaac gattggggtg gtaccattcc caaccatttg aatcaaaatc ttgtagtacc
     2221 tcatataatt ctctctgaaa ctgatacatt gctattcatt ataggtgata caaacaccgt
     2281 tttagtatca ttaaacatgc ctactgaacc aatgtaagca ggtattacct tatggttttg
     2341 tgcaaacccc tttagcttgt ctatgcaaca agcctcatcc acatcattga aacaagccaa
     2401 agtaagatga ggtcttgttt tccattctaa gtacttcgta ctaatttttt catcagcgat
     2461 ttttttagat aaatcaaaaa gttgcttttc cgttttttta tcataatata gctctatcgc
     2521 atattgcata ataacacctc caccataaag taaaaatacc tatctataaa aacacacaaa
     2581 tacctatcat tttaatatta tattactact gttcctcttc caattttcta tatagtgtag
     2641 ccaaacctat gcctaagtct tttgctatct tcttcttcgt ctttgtatct cttccatact
     2701 tattcaacaa cttgtttatg taagtcaact ctaattcttt aattgaaagt atcctttctt
     2761 gattctctac tatccctcca actatatcat cttcaaatat tatatttaca tccttatttt
     2821 tataaatatt tccattaata ttataatagt ttaaaatatc taatggaagc atatctttat
     2881 atattattcc atcatctcca accaaattca tcatatattc aactgcattt tccagttccc
     2941 ttatatttcc cggccaatta tagttaatca acatattcat tacattttct tctattttat
     3001 ttaactgaat acctaattct aaagaatatt tagaagcaaa cttcatcatt attgcctcaa
     3061 tatcatcctt tctttcccta agaggtggca aatttatagg aattacattc aatctatagt
     3121 ataaatcatc cctaaactta ccttcattta ctaatttcaa taagttttta tttgtagcag
     3181 caatcactct tatatctaaa tctataagtt tatttgaccc aattctcgca aattttctct
     3241 cttgtaaaac tctaagtaac tttgcttgta aaggcattga caaatctcct atttcatcaa
     3301 gaaatataac accttcattt gcaagttcaa atttacctac tttccctcca ctactagctc
     3361 cactaaatgc accttttata tagccaaata gctcactttc aaggagattt tctggaatag
     3421 cagcacagtt tatcgcaata aatggcttgt tccatctgct accttcagca tgtatagccc
     3481 tagcaattag ctctttaccc gtaccacttt ccccagttat aagtacagtt gaatttgact
     3541 ttgctaattt cttagttctc tcttttactt ttaacattgc ttctgaattt ccaattatag
     3601 actctatatc actatttccc cagccactat tcacttttcc atgtccttta tgattgatat
     3661 atgtcttgtc aaatattatt attttgtcgt attgatttat atatggatat accggaatta
     3721 ttttggcatt tatattataa attttgttat caatatcaat ttctagtaat tcatgtccat
     3781 ttgaggaaga ttcaacagat actatattta ctatattttc tattatattt ttatctatat
     3841 ctaatttttt tagagctata ttgtttatat aagatatttt gctatttatg tctagtatta
     3901 taacgccttt ttcaacattt tcaatgattt ctatcatcat atccattttt ctactgctat
     3961 tttttgacgc ttcatgttct tctattttca tagaaattaa atcgcatata tgattgacaa
     4021 attttaaata attatctatt tttgcactta tttctacttt tttagtttta tctgtgctaa
     4081 tagcaccaat tactcctata gttcgattat tatactttat tggaactgct attattactt
     4141 tatttaggca tttcatgtaa tgggaacatt ctatacaaag ctgattatca catatatcca
     4201 atacgactaa ttcctgacct gtctgtataa cattgtcata aataaagccc tcagtaacaa
     4261 cgctttcatt tatctttgat ttatacagac ctgtaccact aattcttatt aatctatcgt
     4321 caactatttc aatatctaca tctaacacac tagctactgt ttcagtgtat tttatcattt
     4381 cttgttgaaa ttcttttaat ttacttaaca tagtttcccc cattagaatt taatttacca
     4441 aattatcagt ttagctatat tttaacatat agtcactctt taaaatatat ataaaacatt
     4501 acttatttat atatttgcgt taaaacagac aatattaatt tataaaaagt ttgttttata
     4561 tagtaacctt aattataata tgcagacaat aaaataaatc acaatacaat aataaaaaaa
     4621 agctttcctc atataacaaa tatatttttg acataaaaat atacattata tgagaaaagc
     4681 tcaatagtta aatatttaga tagattttta tatagacttt tttaatataa acttttttaa
     4741 ttgtagactt tttttattag tttttaattt ttattagttt tgttattggt tttattgtta
     4801 taatacttat aatactgcca ttgcttcaat ttctaaaact gcatcttttg gtaattgagc
     4861 aacttgtaca catgaccttg ctggtggatt ttctttaaaa tatgtagcat atacttcatt
     4921 aatatcatta aaatccttta aatcttttac gaatactgta gtcttaaata ttttatccat
     4981 acttgtacca gcttgttcta atattgcttt tacattatct aaagaagctt ttgttgcttc
     5041 ttttgggtca gatactaact cgccagtctt catatctatt ggaagttgac ctgatgtaga
     5101 aactatgttt ccaacttgaa ttgcttgaga atatggtcca attgctcctg gagctttctc
     5161 tgtagctatt actacttttg acatgacttc acctcctatt attaagttat atttacagga
     5221 aaataaaatt tcctaaacac caatattaat acgaaccttt agattaaact tcaaatctaa
     5281 aacttcaaat aatcaatact tagatgatta tgtttatttg tgtcttgtta acttttatta
     5341 aagcaattaa cgtgccaaac tttatttata tataaaattt tgtgaagtca ttcaaaatag
     5401 ttacttctga attttttcgt aaaaaaacgc tctaaaatta cttttttatg atgtcaattt
     5461 ttttctcata tctagaaatt taccaacatg agaattcttg ttttgagaat ttacagatta
     5521 aagttatttt ttaaccttaa attctgaacc tcccatgact aaagtcacag ggttcctgct
     5581 tcatagaatt ttgcataatc aagttattac atatgaaaga actatagttg acaatcccac
     5641 tattataaat aataaaacct ttacccatac cttatttacc ctagttatca tagagcctat
     5701 tgaaaatata acagctaaat ataataataa ccaaaaaatt atcaatatct accactgttg
     5761 ctgaaaatcc aaaaaactac cttttgatag tattacttaa caatctaatt aatctcataa
     5821 taaatttaca acttataaat aaattgttat attggccgta tctcaacaac tttctccttt
     5881 cctagtttta aatcttttat agaattgtac cacgtctaag taaattttca tactattaag
     5941 tcttatttct aataaatcat tagctctcat tctatgccca ataatgtaag attgcagtaa
     6001 ttttattgat tttaatagtt ttatttaata tacttatatt aattattttc atatgaaatt
     6061 attatttata tagggggatt tttttaatat gagcaaaagt gaattaacag cagaaacaac
     6121 agaagaaatg ttagaagtac taagtggtaa agattatgat attgcatgtc atttacatga
     6181 acttggtaaa tcattagatt gtaaaattga accaaaaaca ggtgctcgtt cttacaaaat
     6241 agtatattca actaagaaac caaaacgtag cttatttact attgaatgta atgaaaaaaa
     6301 atggagagtt aaagcaaatc tttttcatct aaatacatat aaagatgctg tggaggaatg
     6361 ctctaaaact attaaagata gtataactaa aactcgtact tgtaagaaat gtaattcaaa
     6421 gtgtattgga ggttcttcgt ttgaattaga tggaaagtct tacctgactt gcataggaag
     6481 tggtcattat tttgcaaata tggaagaaat ggattggaaa aacctagaaa aattaattac
     6541 taaagaaaat aatattatgc aggaatctgt atagtttttt aagggtaatt attaaaatat
     6601 atgagtaaca acagatactt aaataggcat aaaaaaataa agggtataat aaggatatag
     6661 aataaaaata aatgatatag cacaaagagg ctgtctcaaa atacagataa gcctcttttt
     6721 attgttgaaa tgataagaat atattcaatt ttatacaatt ttatacaaac atactacgcc
     6781 aacttaccca tttcatatgc ctgtttcatt gccttacttc ccttaatatc acctacattc
     6841 caagcaccta caccatatac tatacctttt tccttagaac catttagaca tgatgtaaat
     6901 cctctaaatc cttctattgt tctttccata tcagccacat tttcatctgc tgctgtaacg
     6961 ataaaataga aatctttatc acaaatttca gtatatctag agcaagttct atcaatcata
     7021 gttttcattt gcgcattcat agtataaaaa taaactggag tcgctaaaac aatcacatct
     7081 gccccaatca ttttttcaag aatttctccc atgtcatctg tctgtgaaca atttgcttct
     7141 ttttcataac aagcaccaca tccagtacaa tagttaatat ctttatcttt taaaaatatc
     7201 ttctccactt gatttttaga ttctaatacc ccttgtataa actggtcaca caataaatca
     7261 gaatttccac cttttctagg actagctgat actattaata catttttact cataagatac
     7321 tgcctccatt ttcttatttc tactgctctt taatactttt tctttctcaa taataccttc
     7381 ttcatatcta tcaatcttaa aatttaatcg ttccaaagtt ttttgcattt cttctattct
     7441 ttttgctagt tggtttctct gctctattaa aagttctttt ctagctttga tagtactgct
     7501 cccttgttga aacatagcta catactcaat taatgtctca atagaaagac ctgcacctct
     7561 catacacttt atgaaattaa cccactcaca atcttcttct gtaaaagacc taattccact
     7621 tttatttcta tttacaggtg gtattaaacc aattcgttca taatatcgta aagtatccgc
     7681 tgaaagctcg tattttttac taacttctga aatcgtcata catatcttct cctcttgata
     7741 ttgtatagat tgaattattt ctcaaaggtt tctgctacat tctttaaagc atctataatt
     7801 ttaatgtgtt gaggacagct ttcttcacat tgtttacatt caatacaatc tgaggccttt
     7861 ccataagttt tagtataatt attatagtag accatctgtg ttgaaaatcc agtattaaga
     7921 gcttgttttt ctgcattgta caacgcaaaa tagtttggaa tagcgatatt ttttggacag
     7981 ccttctacac aatactgaca ggcagtacaa ggaattaaaa ttgattcgtt gataatctct
     8041 actgcttcat ctataatatt atattcctct tgtacgaatg gtttgaaatt ttgcatatat
     8101 cctgtattgt ctaataattg ttccatattt gacatcccac ttaagaccat cataacatta
     8161 tctttacttg ctgcaaaacg tattgcccaa gatgaaattg acatatctgg attgtagtca
     8221 ttcaataatt tctctgcttt ttctggtatc tttgcaagtg tacctccttt tactggctcc
     8281 ataacaatta caggcttatt atgcttctct gccacctcgt agcattttct agattgtatg
     8341 ctttcattat cccaatctaa ataattaatc tgtaattgca cgaagtctac ttctggatgc
     8401 tcactcaaga ctttatccaa taattctgca ctatcatgaa aagaaaaacc aatattcttt
     8461 atttttcctt cttttttctt ttcttcaata aacttaaagc tatcaaattt tttagctact
     8521 tcataatgtg acacgcctat attgtgtaaa agatagtagt caaaatagtc tacaccacat
     8581 ttttctaatt gctcattaaa aatggtttct tgctcttctt ttttctttaa agccattaat
     8641 ggaagttttg tagctatagt aaaactctct cttttgtgtc tttttactag agattctcta
     8701 agtgctatct cactttttcc catgtgatac acataggccg tatcaaaata tgtaaatccc
     8761 ctttctaaaa aagtgtctac cattttattt aaatgttcca tatcaatagt tgtaacatca
     8821 ttttcatctt ttattggtaa acgcattaat ccaaatccta atttttttgc ctccattgtt
     8881 ttacttcccc ctttaaaact tgtttgtttt ttctctctac tttaactatt cttacaagta
     8941 tataattctt gtataattat actttactac ttggagttaa cttcaagtca atagattttt
     9001 ttatttttaa gacaaatatt ataaaaatac agttctttaa tattaatcca ttacatgttt
     9061 ctttcaccct ccaattctca aacttcacaa actccaacat gtaaagaaat atatacttta
     9121 agaaattcag tccattaata ttactacaaa tactttttag atatgtttaa aaagaaattt
     9181 cacctaaaat atttataaaa gatttagaaa taggtcttgt gtctatttta aaatatataa
     9241 aacagtgata aagacttatt tataagataa aaaatgaata gtgtataccc tttttaaaaa
     9301 gttttttcct ttttatattt gtgatacaat gttaaatata aaaaggaaaa ttttgtatta
     9361 aagaaatctt atttgttgat tttgtaactt caaactcagt atcaatttga ttttaagatt
     9421 atattttatt aagtatgcta aataacaaat ttttaaagat tgtgaggtaa aaatatgaat
     9481 aagaaaaatt tagctatatt aatatcaggt atgatgtttt tttctagttc ttcagcaatt
     9541 tttgcagaca atactacaaa gcaagaaaga ttaataggta aaacaagata tgaaacagca
     9601 gtagaagtaa gtaaattggg atgggttcaa tcaaaaacag ccattatagt taatggtaat
     9661 tctattcaaa gtgcgctttg tgcaaatcca tttgctaagc taaagaatgc acctatactt
     9721 ttagtaaaca acaacagtat tgaaaattca actaaggctg agcttaaaag gcttggtgta
     9781 gataatgtgt acattgtaga tagtggaaat agcatttctt ctaaagtaga aaatgaaata
     9841 aaatcactta acataaaaat aaataaaata gtaggaaaca atatatatga aatgtctaca
     9901 aatgtactta aagaaataga taagataaaa aagatagaaa atgttgcagt tgtaaatgga
     9961 ctaaagggac tatctgatgc agttagtata gcttctccag cagcaataca taatatgcct
    10021 ataatattag tatcagatga aaacagcaat gatagtagta taaatttctt aaacaatagg
    10081 tctataaaaa aatcttacat agttggagga aataagattt tatcagacaa tacagtaaag
    10141 aaatatccaa acagagaaag aatcagtggt tcagatagaa acaatactaa tgcaaaaatt
    10201 attgataaat tttataaaga taaaaaaata aataatgtat ttgtagctaa agatggtatt
    10261 aaccaagaaa atgcattagt agattcatta tcaataggag tactagctgc taaagaaaat
    10321 tctcctataa tgatatctaa tggtagcctt gtaaatagtc aaaaagaatt tattaaaaat
    10381 aaagatatag atgaaataac acaagtagga ggaggatata atcaaaaagc atttgaagat
    10441 attttaaaac taaaaaatgt gaacataaga aataaaacta aaaaaatatc tttaatacag
    10501 gctaaagaaa tattaaaaca agctcttcca ggaagcaata ttttagtttg ttattatgat
    10561 gaagatgata atgaatatga ctgcaaagtt ataaaggata atatcctata tgagatagaa
    10621 attgatgcct actctggtaa aataatagaa attgaaaaag atgatgactt aaatgataat
    10681 aatttaggta cacaaaatat atcattggat aaagctaagt ctatagcatt agatgctatt
    10741 cctaatggta atttaatcta ttgtaaatat gatgctgaag ataacgaata tgaggctaaa
    10801 gtaataaaaa ataagaaaac atatgaatta gagataagtg catttgatgg aagaattgta
    10861 gatattgaca tggacaatga ttctgatgat gactttgatg ataatgataa gaataactct
    10921 aatactaatg acaacagttc tattattagt gctgaaaaag ctaaatcaat aatgctagat
    10981 aaagtgcctg gtgggaaaat agtaaaattt aagtatgatg aagatgacaa agaatatgaa
    11041 ggtgaaataa taaaaggaaa aactgaatat gagataacaa ttaatgcaat tactggtaaa
    11101 atattagaat ttgaaataga tgattaataa aaaatataaa tagatgatta ataaaaaata
    11161 tatattctat taattattac aagcaataaa gccataaata ttgaaatttt caatatttat
    11221 ggctttattt tattttttta atttaaatcc acattatgcc attatatata tctatgcact
    11281 cttgtaaaat gggttatatt tctccagtat attaaaaaaa gtgagtgtct caaaataaat
    11341 cttctaactc ataaagcact ctcctttgta ttaaatcaaa tatattttca tcatttaaac
    11401 aattaagaag agacttctct ctattttgag acaactccat tttaatacac tatttataca
    11461 taaacatatt ttaaacaact aaaaagcaac taatatctta ataaatatct caatacatat
    11521 cttcataaca catatcacca taattacaag cagataaaga actctcctca tttaaatacg
    11581 actcaaattc atacccaaac agaattgttg gttctatata tatttccata tctgtatatc
    11641 tccatcttct tacctttgcc tctataacct tgtaaaaatc ttctaaacta tacccttctt
    11701 ttaatctttt atctataagt gccttagtta tactcaaatc agctttaaaa ttttttcctg
    11761 attcactatt taattttctt ataataattt catgtatgct ttctggattt ttgtaagtag
    11821 aatcttcagt caaatcttca tccgaatttt caaaggtatt tttatttaaa ttctttttat
    11881 ttaaattatt ttttaataat tcttttttct ttgtctcatt ttctgtttca tacccagtat
    11941 gaattatcaa atcataatct ttgtctatac tctcttgatt tatacggctg tatatgttgg
    12001 tattactgtt cttaaacaac tcatttctat gtttatattt cattgttttt tggttattgt
    12061 taatagttgc atcaatgtat ttctctatat ttttgaaaat atcaccattc tttgttacaa
    12121 tttctgcatt actactttca gaacaaacat aactatatac agaacaacaa tttcccttta
    12181 ctcccctact tattaatctc aatataccta aatctataaa ttcatttaca agtctactta
    12241 ttgtagatat agatacatca aggtcagatg cagctttcct tactgtcatc ataaattgtc
    12301 ctcttggcag agttcctttt aatctttgta tattttcttt tctcattaca tagtgattaa
    12361 accttattct atttacatct cttttttcat taaaatataa tactgctgaa tttaattgat
    12421 aaaaactatt actcatattc ccactcctcc aatttaaata tataatttgt tgtaaagttt
    12481 ttagattttt aaataactta ttaaatgatt atttttatta aaaagtattc tttgatattt
    12541 tcattttaaa gttttaagta taataagtta atagtgtaga tttaactttt ttatttaaac
    12601 aataaactta atattatttt taatataaaa attttattaa atttagtatg taatttttta
    12661 atcttatttt attccatcta tactcttatg ctaagtaata actttgataa tatttcccta
    12721 acttatgttt caactggttt atattgcaat ttatttaatt acagcttatt tgcataataa
    12781 ttcgtccata tcataagttg tgtatttata taaatacaca attaaaaata aatcactatg
    12841 gtatactaat tattacgaag actatactta tatgcaaaga caaatataag gcatcttata
    12901 tcttttatca attataaaaa attaaacgct atcttattta tcataaatta caaaaacata
    12961 aaattttatt actatattgc tataaattaa ttgatacaca ttttacacaa taaggagggc
    13021 tatatatgac aaatgctaaa agtaaaaaag aatatctaaa tcgtatttgc aaagtgcaag
    13081 actatataga agaacattta catgaacctc tttctcttga tgaactttct aatatagcag
    13141 gtttttcaaa atttcatttt catagaatct tcaaagcaat agaaaaagaa actttggcac
    13201 aatatgtaaa taggctaaaa ctagaaaatg caactgcttt tcttattcat agaactgaca
    13261 tgactgttac tgatattgca cactacttcg gctttactga ttcagcagtt ttctcgagag
    13321 catttaaaaa ttactataaa ataagcccta taaaatatag aaacaattat agcaagaatt
    13381 gcaaagaacc atataaaatt tctcagtaca atagaagcat atcaaaacct aaatgtaaga
    13441 atactagaga ggttaaaggt gaaattgaaa ttctagaact tgataatatg aatactattt
    13501 acactagata tactggttca tatggtaatc taaccagtac tttcccaaaa cttttagaac
    13561 ggttattcac atatgcaagt gagcagaatc tacttggact tgaaaacaca caaatattag
    13621 ccatttatca tgataatcca caatttacac aggagcatca attaaggact agtatttgca
    13681 tgaccattcc agataacatt gttgtcaaag agaatgacaa tcttggcaat atgataatac
    13741 cttctggaaa atatcttgtg ggacattttg atatttcaaa atctgaatat agtgatgcgt
    13801 gggattttat gtataatgaa tggctatcaa atggtaatta taagctacgt gcctccttcc
    13861 cttttgaagt atatcttaac aacccacaca atcaccctca aaatagacac ttggtcgata
    13921 tatatttacc tatagaacct ttttaataac ctcacctaat ctattcttgt attaaggatt
    13981 aatatatccc tattttacgg tttgataaaa ttgataaaat gaggtgaacc aatgaaagaa
    14041 ataaaaatta atggagcaaa gatacacaat cttaaaaata tagatgtttc tattccaaaa
    14101 gataagcttg ttgttgcaac aggtgtcagt ggctcaggta agtctagcct tatgtttgac
    14161 attgtatttg aggaagggcg taaacaatat ctgcaatctt taggtatact tgcaggtatt
    14221 gatagtgaag ataagtttga taatattcaa ggtttagctc ccactatagc tgttcaacaa
    14281 aatattattc gccagagcaa tcctcgttca acagtaggca caagaaccaa tattctcaac
    14341 atgcttacac tattgtattc agtggaaggt caaataatgt gtactatgtg tgatactcct
    14401 gtaagcgata atctaatttg caataattgt ggatatgagg aagaacgatt aagaccaagt
    14461 tatttctcat acaactcttc agatggaatg tgtatgaagt gctctggaca tggtgcatat
    14521 tttgagatta atattgaaaa attagtcctt gataagcatg ataccctaaa acaagtctta
    14581 gatagagcaa agattacacc tggatatttg agagtatttt ctaaaaaatt caatgattat
    14641 ttagacatac aattcttaca actacctgaa gaaataagaa atgaagtact ctatggtcat
    14701 tatgaaaatg gtaaaaaaag ttcctcttta tcaaaggtat ttcacaatcg ttacaaaagg
    14761 ggcgaagatt taaatggagt ttatactatg actgcctgtt cagattgcaa tggttttaaa
    14821 attggtgaag aagcacgtag agtactttta aacggaaaac atattggaga acttggaaaa
    14881 atgacgattt tagaaattga tgatttcctt aaactcttaa taaatcaagg taatttaaac
    14941 acatttggta caaatcttgt aaatgatatt ttatccaaaa ctcaacattt aatagaatca
    15001 cgattaggtc atctatcctt ataccgagaa atgtcaactt tgagtggtgg ggaaatgcaa
    15061 agactatttt taaattcaca tctcgattca gagatggact cattaattta tgtgcttgat
    15121 gaaccaacag ctggattgca tgaatcagaa aaaatagaca tcttaaaatc actaaagaaa
    15181 cttaaagatt taggcaatac ggtcatagta gttgagcatg ataaaaacac aattgaaatg
    15241 gcagaacata ttattgatat tggtccaaaa gctggtgttg aaggtggtca attagtatat
    15301 caaggagatt tagaaggtct acttcaatcc gacaaatcac ttactggaca gtatctatct
    15361 ggaaaatatc caatgcctaa tagaacaagc aataaaaaca ttacacatac tgataaaata
    15421 ccttgtataa ctattcaaaa tgcaaataca aataatctta aaaatgtaac tgtatcatta
    15481 cctttgggtg ctatggttgg aatatctgga aaatcaggta gtggcaagag ttcacttata
    15541 tcagacaccc tacttcctct tcttagaagc caatttaata accatactat caataatcaa
    15601 ataaattctt cagagagtga aataggtgag gaagacgatt atgcagttgt tgaaactatt
    15661 gcagatagac tgattggtac taaacatatt tctggatatt cagagatatc tcaatctcct
    15721 ataggcaaaa atatgaactc aactcctgct tcttatattg gtatatggga taagatacgt
    15781 acactatttg caggtcagcc agaatcgtta gaacaaggat ttacagctgg tcatttctca
    15841 tttaactcca aaggggcttg tcctaaatgt agtggtagtg gatatgaaaa ggtctggttg
    15901 ggaaataatc taagtataga ccatatatgc agtgaatgtc agggaaaaag atttaatgat
    15961 gaatctttat ctattaaata taaaaataaa aatatacatg atattttaaa tatgagtgtt
    16021 tctgaatctg tacatttctt caaagatatt ccaaatattg tttcacactt aaatgtatta
    16081 gaacaaatag gtatggggta tataaaatta ggccaaccta caccgacttt aagtggtggt
    16141 gaatcccaaa gaatcaaact tgcaaaagaa attggtaaaa aacgaaatgg taatatcctt
    16201 tatgtattgg atgagccaac tactggtcta agtctatatg atacttctaa gttgattcaa
    16261 ttattagatg aattggttga aaatggaaat tctgtaattg ttgttgaaca tgatatagat
    16321 gtattaaatg tttgtgactg gattgttgag cttggtccag aaggtggtga caaaggaggt
    16381 tatataattg cagaaggttc tccaaaaact ctaaaagaaa atccaaaatc tataacaggg
    16441 aggtatcttt aatgaataaa aacaattgct tagacttaat ttttgaaaaa gtagaccctt
    16501 caagtgttgg tatgagttca gaaaagcttt cacaacttga acctttaata atctcacaat
    16561 atagcaatat aaatggtata attattgcaa gaaaaggaaa aattgtcttt gaaaagtact
    16621 ataatggata taatgctgat gatacttttc atgttgcatc agttacaaaa agtattatat
    16681 cagccttaat aggtattgca atagataaag gatatataaa agatgttgac caaaaggttg
    16741 ttgatttttt tcctgaatat acttgtagtc cttctgaaat atgtaaaaaa gcagtaacta
    16801 tacgtcacct acttacaatg actgcaccat acccttttaa aaatatgact gagtctttag
    16861 aaagactttg caaacaaaaa gattggataa aatatacctt gaatcaactt ggtcaaaaag
    16921 gcgaaattgg tactttcaaa tattcttcag ctggaacgca cctactttca gctattataa
    16981 caaaaactac tggaaaatct gctcgtgaat ttgctaatga gtacttattt aaacctattg
    17041 gtatgaaagt tataccagat tatgatatga aagattttgg ttttgaagat ttctttggaa
    17101 aaaatcttaa gggttgggca aaagatagac agggttattc tataggtggc tggggactta
    17161 cattgagtgc tagagatatg ttgcgatttg gttttctgta tttaaatgat ggtatttgga
    17221 ataataaacg tattatatct aaatcgtgga ttgaagaatc agttaaaatg aatagtaatg
    17281 aatacggtta tctgtggtgg ctatgtaaaa aagatggtgt tttgtggtat tcagcaatgg
    17341 gaactggagg aaatgctatt tgttgtattc cacaatatga cttagtaata gccataccat
    17401 ctaaaatagt gtttaaacct cgtgatagat gggagttcat tgaaaaatat atacttactt
    17461 ctataataga ttaattactc tttcataaac taattactct ttcataaact aattactctt
    17521 tcataaacta attactctta gatagccaaa ccctccatta taacaccaga cttttgtttg
    17581 gtgttattat gactaatact atatatctaa aaggagttga atactaatgg acacttacac
    17641 gacatcagag gtagcagatt taattggtgt acatcctaat acagtacgtt tatatgaaga
    17701 actaaactta atacctaaac ctaagcgtaa taccaatggt tatcgtatct ttaatgattt
    17761 tcatatagaa caatttagat ttgctcgtac agccttgaaa attgaagtgg tacaaagggg
    17821 attaagaaaa gatgcccttg agattgtaag aacatcagcg aatagggact ttgataaggc
    17881 tattagtctt gtaaatcaat acttgcaaca attagaaata gaaaaatcta atgccgagga
    17941 agcacttact attgttgcac agattttaaa tggaaatact ccagaaacaa ataaactctt
    18001 tttaactaga aaagaaactg cagaacactt aaacgttaca atagatactt taagaaattg
    18061 ggaaatgaat ggtttgttaa aagtaaaaag aaaacaaaat ggttatcgtg tatatactga
    18121 tgaagatata aaacgactta aaattataag cgcacttcgt tgtgcaaact attctctttc
    18181 agctatttta cgaatgttaa atgctttatc ttctagtcct aatactaata ttaaaactgt
    18241 ccttgacact ccaaaagatg atgacgaaat tatctcagtg tgtgacaaac tgataacttc
    18301 attgaaagaa gctgaacata atgcaaaaac catgaaatct catcttcaaa ttatgaaaaa
    18361 acaattttcc tagccctcca ctctaactcc agactttgat ttgtgtgtta tccttaagag
    18421 caagaaaaat tcaacacaca aatcaaagga ggaataggat ggaaaataaa aatactaatt
    18481 ctacatggaa gaaaaaagtc attctttttt tagcaagtca atatatttcc ttatttggct
    18541 catcacttgt acaaatggca atgatatggt atgtcacact tcagacatca tcaggtgtat
    18601 gggtaacagt tttaactgta tgctccttta tacctcaaat gataatttct ttttttgctg
    18661 gtgtatgggc agactactac aatcgaaagg tactcataat tgtatcagat tcaacaattg
    18721 ctatatctac attagttcta attctattta tgatgtcatc aaatggcaca acaagtgaat
    18781 taatagccat atcagtaata tctgttatac gttcactagg aagtggtgta caaacacctg
    18841 ctgtaaacgc aatgattcct caacttgtac cagaagaaca tttaatgcgt tttaatggta
    18901 taaatagtag tctacagtct atagtacaat ttatagttcc tattgtagct gctgcaatac
    18961 ttggatttgg taataaaata aatcaagttc ttttaattga tgtatttact gcaattattg
    19021 ctattgtatt attaatattc atcaaaattc ctaaacatga tataccaaaa caaactgaaa
    19081 aagtctcatt ttttgcagat atggtatcag ggataaagta ctgtttttca gataagttca
    19141 ttagaaaagt gctttttact tatggagcat tcatgcttct ttgtgttcca tcaggatttc
    19201 ttataggact tcttattgaa cgaacatttg gcaagggtta tatatacctg tctattattg
    19261 aaatgattgg atttttaggt atggttttag gtggattgat tataggagtt tttggtggtt
    19321 ttaaaaatag aaataagacc ttttttctag caatattatc ctatgctatt ttttcaatta
    19381 ttctaggtct tgtaacacaa gcttggcaat tttttatatt tatgtttttt acgaattttt
    19441 caattccaat cattcaagca tcagctatga ctatgttgca agagcatgta gcaccacaaa
    19501 tgctaggtag agttcttagc ttacctatta ttatatatac tggttttatt cctcttggta
    19561 tgatgatttt cggaccaatg gcagatgtaa tttctattaa taatttaata atcatagcag
    19621 ggattatact tttatttttt gctttggcta tcccctattc aaaacaattt tacaagcaag
    19681 gaatatctaa gtcaaatgaa tccgagtaat atatttaaat agtttaactc gatttatctg
    19741 gttaaaaatg aaggggctta aaatggactt tttaatttat gaagaaaatt ttttatatga
    19801 aatcaaatat attttatcat ttcaacattg aaaaataggt ttatctttat tttgagtccc
    19861 tctctttata gcaaataata ttttgatact atctttatta actttatata ataatacctt
    19921 tacttgtagg ttataatgat gaaatttatc aaatcatata ataatacctc catttatatc
    19981 ataaattaaa tttatagtac aaatggagga aaataaagta tttatatagt ttaagtacct
    20041 attaagtttt ccactaagta gctctaattg tactgaactt cttactaaat tatattgaat
    20101 aattataaaa taaaactaac tattacacta cataaattaa agtatacaaa ttgcactaca
    20161 taaattacac tatacaaagt atattaaatc acctattaaa ttgtcccaat tataattata
    20221 ctgagttatc tactaaaaga taactttctt tttttatcta aaataatata tatcataaac
    20281 catactacac aatgtacagc caaaattaca atatctttga taataatgac attaccatta
    20341 ataatctcca taataccatt aaatgtagca actgaaggca ttatatagca taaattccct
    20401 ataccactgg tattaacccc tagaagatat attaacaaag gagtacctat aaataaaatc
    20461 attactattt taatataaac aattcctacc ataagacctt ctgacaactt tccaataaat
    20521 aaacctataa gtgatgaaat aaatgctgaa aatacaacga gtaacaacat cattatcata
    20581 tctaatattg aaatatccaa acatataaat acagttatca ttgaagataa acacccacat
    20641 aaaagaccca aaaatacttt ttgtataata tactgacttc tactctgtgg caaaacttca
    20701 tttataaatg atattccatc ttctttttcc gatataatat tcattgcatt aaaggtgcat
    20761 cccataaaca tagcagtaat taaaatcata gcaataaaaa cattttgaaa ctcttttaaa
    20821 atactatctt taggcaatat tgtaacctca acatctttta aatcaccctt tagtctaaac
    20881 atctctggca atgtctttcc aacatttttt atagcattaa tctcatttcc ataaaacaca
    20941 gtttttattc ctttaccatc tctaacaact ccaattgtgt ccgttttaat atccttaatt
    21001 tcacgtatta aatgcatttt tgtgtcatat acacttacca taccatactt ctttaaccac
    21061 tgctctgttt gtacaggaag tgtatccttc actataccaa aacgtatctc tcctaatgaa
    21121 gatatatcaa ttgttcccat aaaatttatt aaaatcgcca taacaactgg taaaaagaag
    21181 gaaattatac acattttatc tttttttata ctctttaact ggtaacgtaa tcctatcaag
    21241 attatccctc cctaaccaat tctttattaa aaatatattt tacaagtaaa aatagaacta
    21301 ttattgtcaa agaacacact acaaaataaa ctaattcttt tgaaaaattc atgaaaaacg
    21361 catttttaag ctgactataa acatgataga agggatgata tttaatccaa tctatttcaa
    21421 aagatgtatt tgcagataga aatataggtg tagttatgaa tacagcaacc acaagatata
    21481 taagagaaaa ttgcttaaaa tctctcaata acattgagca tcctattcca atcaaagaca
    21541 taataattga taaaatacta gcttgaacta atacttgtgg aagtattttt attccttcta
    21601 aaaatccaat attaaatact actaaaagaa ctgtaaaaca taggtcacat ataaaaaaag
    21661 caaatatctt tgataatata aaaagacttc tttttacagg taaaatacca gtaatacgca
    21721 ttactcccat ctgcttttct ttaaataata aagaggcaat ccctaaaaat ccaatagcta
    21781 caatttcaaa gaataaaatc tcacaagtca tgtcttttct ttgttttatc tcagatgtat
    21841 ccttatcatt aatttgaatt attgcattgt tgtctgatat tttgctatta tggtcttttt
    21901 ctgatgataa taatgacaat gcatagtcag ctctataatt gtcaactcct ttaatacttt
    21961 catgtaaaat tactttcggc ttactggaac tcatatcaat acaaactgca cttgtatctt
    22021 catcaagttt tgcttttaat tcacctatag aagaaacttg attaattaat ggagatgcac
    22081 tcatgcttat attctctgga ttatatagat atacattatt gtgataacta tcttcatcca
    22141 taaactttac ataaccaaaa ttaatataac acgaataaag tatgagagag ccaagtgtca
    22201 gcacaaaaaa tttaccataa atcatcatcc aaaaatcttt ttttactaag gtaaaaaatt
    22261 tagaatacat tacactaaca tccttcctgt gtatttaata aaaacttctt ctagtgtagg
    22321 ttcttttgaa tgtacactta agatttcatc atatttaaaa gttattccag attctaactc
    22381 ttgtatatct acaatttcaa tatctcgatt tccattatat acaaattcca cttcaacttt
    22441 attattgcta tttttcattt ttaaattttt gggtttatct attaccataa tctttccatc
    22501 tgcaataaaa gccacattgt tacacattaa atctgcatct agcatattat gagtagtcaa
    22561 aaagatagtt gttccttttt ttcgttcttc ttcaataatc ttacgaaata gtacagctcc
    22621 agatgggtca agtccactag taggctcatc taaaaataac aactttggat tacttaccaa
    22681 tgctcttgcc atgctaatac gttgcttcat accttttgag taggatgata caggcttatt
    22741 tataaactct ttcttaaact ctaacaaatc taagagttca tatatgtctc tcctttgatt
    22801 tttaggataa aaagacgaaa aatattctaa attatcaatt gcacttaaat tggtatataa
    22861 ataaggaaat tcaaataaaa caccaatctt ttgatataaa gattctgtgt atgctttaac
    22921 ttccttacca aaaatggata cgtgaccatt gtaacctttt aaaacaccat ttaagatttt
    22981 ctgtgttgtg gattttcctg aaccattagg tccaaggaat ccaaagattt ctccttcttt
    23041 tacttcaaaa ttaagtccat gcaatatctg tttatctttt ccataagaaa attttaactc
    23101 tcttactttt atcatctatc ataacctcct ttatcatttt tttgtcgtct atcccaccat
    23161 tttataaatt taattttaag cggaatagaa agcaatatta tcaatataat agtcaaccat
    23221 gtataccatt tcataagtac tcccccttgt aaccttattt aattacgctt ctatttttta
    23281 atttatttaa cctattttat ttctatttgg tgaaatcaga gtgtctctct tgtgaaatcg
    23341 aagtgaaata ataaaaagct taataaaaaa tcttgttttc atcaggaaaa caagactttt
    23401 tattaagctt ttcattaagc tttttattag actttttcat taagcttttt cattaagttt
    23461 ttttatttta ttctctttaa atacttacgt atacaaataa aaatatttta catcagatac
    23521 ttataattta taaattaata ctaaattatt catcaattgg aatcataaaa taaaactcta
    23581 ctcctctatc acgattattc actccatatt ctaacccttg cattgtaagt atttgtgaaa
    23641 caattgcaag ccctagacct gaacctcctg tacgctgaga ttgaacacta cgataaaact
    23701 tagaccaaat ctttgaaatc tcatcttgct ctataaacct accatcatta taaatttcaa
    23761 ataaaagcaa gccattttca catgtaatcg ataaatctaa attaccatta tatacaacat
    23821 gcttctttgc attaataact agattctcta ctacttgttc cattctcatt ttatctgcca
    23881 ctacaaaaac ttcatactct ggcaaatcat atgtaaaata aaaattggca tctggtatat
    23941 caattaaaag acgacctgcc acagtttcaa caaactctat aaaatcaaaa cgctctgggt
    24001 taagtttaga aactcctgct tccaatgcag ataagtctaa taatgataca atcatattgt
    24061 tcattctatc agtttcttct attataacat tcatataatt tttctttttt tcttcatcaa
    24121 cttcatctat aagcccctct gtatacgcac gtataatgcc aagtggagtc ttcatttcat
    24181 gtgaaattgt atctaccaac tcctttcttt gttccaatag cattctttcc ttatttacat
    24241 ccttttcaag ttgaatattt gcactttcta ggtctgataa tgcctgttgt agatttgtag
    24301 acattgtatt caggctccta gaaagttctc caaattcatc attagtattt acagtacatg
    24361 gattagaaaa atcaagttgg gacatacttt ttgcagattt atttatagat gtgattggtt
    24421 ctgaaataaa ttttcccaat aagaaatcaa tactcataat aaatataacc agtattataa
    24481 accaaatcca aaattcaaat ttagagttta tcgaaaattc ttcaacaacc agataaaata
    24541 atataattaa cgctcctgat aatttagaaa gtaaaaacac tttttttcta actgaatata
    24601 gtccaaaatt ttgtttaatc caatttgaaa tatctacatt tttcagtttt tctataatct
    24661 ttcttaaact attcattcct taacctcaaa cttatatcct gaacgaatta atgtaactat
    24721 atggtgtcct tcactaccaa gctttttgcg taaggttttt atatgtgtat ctacagttcg
    24781 tgtgtttcct tcaaaatcat atccccatat acaatttaaa agtttctctc tggaaaaaac
    24841 ttgattaggg ttttccataa ataaacgcaa taattcaaat tctttatatg ttaaatcaat
    24901 caatttatct tctacataca cttgtcttga tgctaccata ataaaaatct ttcctaaact
    24961 caatgaattt ttgggaatat cagtagtgta tctacgtaac aaagcattaa cttttgctaa
    25021 aagtactttt gggctaaatg gttttgttat ataatcatct gcgccatatt cataaccctt
    25081 taatttatcc tcctcttcac ctttagctgt caatataata ataggtatat tagtcatttc
    25141 acgtgaaact ctacacactg aaaatccatc taaatacggc atcataatat ccaatatcat
    25201 taaatctata ttgttgctct ttaatattgt aagtgcctcg actccatcat cagcagttat
    25261 acaagtatat cctccctttt gcatatattc tacaataata ttttgcattc ttttttcatc
    25321 ttctacaact agtattttag ccatttctat ttatcacctc ttttttgtag gctttatttt
    25381 agtttatatt aatttattta tttttcaact atttttttat agtatttttt atagcatttt
    25441 gatagtattt tttagactat tcttaatgga attgatatca tagataaaaa tagcctacta
    25501 actttttcta tgttagcagg ctaaaatatt tctaataata attatattat tttgtcacta
    25561 ttctattttt tcgattaaaa tatattctaa agcaaatatc ttcaattaat gaataaattg
    25621 cacttgttat aaagtacaga cctatggcta ctggagttct aacagttagt ataagacttg
    25681 ttatggtagt caccacagcc atttgtttgt taaactttac ttgagaattt atttttaaat
    25741 atggtatata attcactaaa tttgaagcta acataattaa tgtatatata caaggtaata
    25801 taaacaaatt atcaaatgta cctaaattag caatccatgg aattataatt gttgtaaatt
    25861 cctttgtcat atctaaacat acatgatata aagcatatac tactggcatt tgaaccaaaa
    25921 ctataagaca tcccagcata ctcttcatac tttctacaga acttacttgt atttgctttt
    25981 ccaattcttt agtatcattc ttatactttt cttttatata ttccattttt tcagctaatt
    26041 cttgctgttt ttttacagaa aatctttgtt ttaatgatag tggcataagt actaatttaa
    26101 ctattaaagt tataagtaca atagaaattc caaaatcacc tgtaaaatta aataatatac
    26161 ttaataatcc ttgcaatata cttgaaatga aatccatttt attctctcct ttttattttt
    26221 aaactttaga atttataata gattcatttc aaattaaaac aaactacaat acctccaaat
    26281 atcatatttc tgaaaaacta tatactgttt aggcagtcgt tcttcagaat atgtaattct
    26341 atttacaaat atgtctctta ttaaaacata cttaatttta tgatttaaga ggtctttaca
    26401 aaaataaaat atacttccaa tgatagatac caataatgtc atataaatta gtacatataa
    26461 catactatcc atatctaatc tcatcacatc acctcttcat cacttgtatt tatttttaga
    26521 caatttcaag tcctaaataa ttccaacatt tatatttagt tttaaataat tatagtatag
    26581 aaaactccta atgtaaatat atatattatt atatttatca tttttattcg tcatatttac
    26641 atttataaag tatataattt tatcactctt aatttttaat aaaaatcacc taaagtattc
    26701 tttttttatc tctgaacaaa acaatattaa gtttttatag cttattttca aattatagtc
    26761 ctta
//
LOCUS       DAMJWS010000057        25870 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMJWS010000057 DAMJWS010000000
VERSION     DAMJWS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.057"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            96..359
                     /locus_tag="PC399_002420"
     CDS             96..359
                     /locus_tag="PC399_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593276.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNYENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(796..1173)
                     /locus_tag="PC399_002421"
     CDS             complement(796..1173)
                     /locus_tag="PC399_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2593277.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            1514..2626
                     /locus_tag="PC399_002422"
     CDS             1514..2626
                     /locus_tag="PC399_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2593278.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(2834..3310)
                     /locus_tag="PC399_002423"
     CDS             complement(2834..3310)
                     /locus_tag="PC399_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593279.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGDHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(3509..5134)
                     /locus_tag="PC399_002424"
     CDS             complement(3509..5134)
                     /locus_tag="PC399_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2593280.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(5472..6491)
                     /locus_tag="PC399_002425"
     CDS             complement(5472..6491)
                     /locus_tag="PC399_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF2593281.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(6488..7057)
                     /gene="mocA"
                     /locus_tag="PC399_002426"
     CDS             complement(6488..7057)
                     /gene="mocA"
                     /locus_tag="PC399_002426"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF2593282.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            complement(7164..7886)
                     /gene="yqeC"
                     /locus_tag="PC399_002427"
     CDS             complement(7164..7886)
                     /gene="yqeC"
                     /locus_tag="PC399_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF2593283.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLVENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            complement(8069..9346)
                     /locus_tag="PC399_002428"
     CDS             complement(8069..9346)
                     /locus_tag="PC399_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2593284.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            complement(9485..11791)
                     /locus_tag="PC399_002429"
     CDS             complement(9485..11791)
                     /locus_tag="PC399_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2593285.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            complement(11784..12578)
                     /locus_tag="PC399_002430"
     CDS             complement(11784..12578)
                     /locus_tag="PC399_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2593286.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            complement(12685..14043)
                     /locus_tag="PC399_002431"
     CDS             complement(12685..14043)
                     /locus_tag="PC399_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2593287.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            complement(15391..16398)
                     /locus_tag="PC399_002432"
     CDS             complement(15391..16398)
                     /locus_tag="PC399_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593288.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEITVMKEDMSEVKQ
                     EINVLKVDMSEVKQEMTVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEM
                     TVMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNF
                     MQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLL
                     HNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSV
                     K"
     gene            complement(16628..17713)
                     /locus_tag="PC399_002433"
     CDS             complement(16628..17713)
                     /locus_tag="PC399_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2593289.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(17749..19062)
                     /locus_tag="PC399_002434"
     CDS             complement(17749..19062)
                     /locus_tag="PC399_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2593290.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLNENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDTKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(19063..21354)
                     /locus_tag="PC399_002435"
     CDS             complement(19063..21354)
                     /locus_tag="PC399_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2593291.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGSDKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(21344..22138)
                     /locus_tag="PC399_002436"
     CDS             complement(21344..22138)
                     /locus_tag="PC399_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2593292.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(22177..22623)
                     /locus_tag="PC399_002437"
     CDS             complement(22177..22623)
                     /locus_tag="PC399_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2593293.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(22695..24101)
                     /locus_tag="PC399_002438"
     CDS             complement(22695..24101)
                     /locus_tag="PC399_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2593294.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(24119..25489)
                     /gene="hydA"
                     /locus_tag="PC399_002439"
     CDS             complement(24119..25489)
                     /gene="hydA"
                     /locus_tag="PC399_002439"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2593295.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEEIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(25622..>25870)
                     /locus_tag="PC399_002440"
     CDS             complement(25622..>25870)
                     /locus_tag="PC399_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2593296.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIKTIG
                     FGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSVYGK"
BASE COUNT         8169 a         4785 c         2681 g        10235 t
ORIGIN      
        1 tcttttttag aatttaagaa ttaaaattct ataattttaa aacatattga atttattttt
       61 ttgagatgat ataattttct taagaggtgg ttgatatgat aaaaaataca aaacaaatta
      121 cagtagaagt taatgaaatt ttattacaag aaatgtctaa gataactaat tacgaaaata
      181 atattttgac agatttgata aatgaatcaa ttgtaaatta tattcttgaa aacaaaaaac
      241 ataatattga aaataaagat ttagaagaag tgattaaatt ttataaaaaa gaaaatactg
      301 aaaaacctaa aagtgcagca agagctatag ctgaagcaaa aactcatact ctgtattaat
      361 caaataaaaa agttcctaaa aactaggaac ttttttatat taataattat taatatatct
      421 caatatttat ctcaaatcta tcaatataag catgtttaat acaatctatt gtatacttat
      481 cttaccctag aaataatcaa aacaaaaata aaattatgcc ctttatatct aaaacaatta
      541 atttacatga ttaatataca aaaattctga cttttaaatt cgtatacaat atcaaactaa
      601 aataatatta atgacaaacc caatataaat taaattcaaa caggtttgat tttacatcaa
      661 acttattgta ttaatattaa ataacaagac aaaaatattt ataaaaatca caaattttac
      721 ataaaacttt atatttgatt catttaacta attattgtaa aaactattta ttttatgata
      781 attaactaca ccaaattatt catctttttt aatatcttta acccattttt caacagagtt
      841 caattcttct tcatttaccc cctttttgaa caaggatttc ataaaactct caatagaact
      901 tccatgcata ttatctaaaa aagtttttgt ttcaaaatgt aaatactctt cttctccaat
      961 aagaactgta taatgtgtat gtctatcaat ttttcttgca cttaagaatt gcttatttac
     1021 taatcttgat aaaagtgtta gtgttgtagt ttgcttccaa ttatatagtt tttccatagc
     1081 ttcaatgaca tcttttgatg ctactgtaaa atctgaactc caaataaatt tcatgacttt
     1141 taattctgct ggtggtatct tagtaatagt catatgattt tacactcctc tcaaacttct
     1201 acataattaa atactatatt aatagacttt tcgagtcaaa ggttttattt aaaatatctc
     1261 atccaaattt attaatacaa aaaatttata tttttattca aaattaaaca cacaatctat
     1321 taatataaag ataaatatct attctataag tataacagat tatcaaatta aaattctaat
     1381 atttgacata atatatacat tttttaaata aatatataca atgtattaaa atgtatgcaa
     1441 ctctatttca aaattatttt gtgatatata tatgttataa taaatgtata aaattttgat
     1501 tggtgggtta tatatgaatg catttattag aaaaagaaat aaaaactatg ttgtttacct
     1561 ggaatttgaa gatgaaaaaa caggtaaaag aaaacaaaaa aatatgggta cttttgataa
     1621 aaaaagggaa gcaagtaaaa gactaaatga ggtaaaagaa agcttatata atgatggttt
     1681 tttagtacca agcgaaatta cagtgtcaga atttctatta gattttctaa ataaatatag
     1741 tgaaaatata tctttagcta catataataa ttatgttagt atttgtaaga attacattaa
     1801 tccatctatt ggaaaatata aaatacaaga cttacatccg gttcacatac aaaactacat
     1861 agataaacta tcttataaac taaatcctca atcaattaag attcatataa atatattgaa
     1921 acttgcaata aaaagagctt atagactcaa attaataaaa gaaaatgtta tggatagtat
     1981 agaggctcca agatataaaa aatttaaaaa tgagatttat gatagagaac aaatgataaa
     2041 attattagat ttagcaaaaa atacagaaat ggaattacct attaatttgg ctattggatt
     2101 aggtcttaga atttcagaag ttttaggtct aacttgggat aatattgatt ttgaagaaaa
     2161 tacaataaca gtaaataaga taacttcccg aattaatggt tctgttattc tcaaagagcc
     2221 aaagacagaa agctctgtta gaaaaatatc ttctcccaaa gaattaatgt ctttattaaa
     2281 agaatataaa ataaaacaga ataaaaattt attaaaatct tccattagaa ataataataa
     2341 tttgttgttt ttcaataaga aatgtgaacc tattgctgaa gatgttatga gtaaaaaatt
     2401 taaaagattt ttagaaaaga atgaattacc tcatattaga ttccatgatt taagacactc
     2461 acatgttact ttacttataa attctaaagt acctataaag gttatatccg agagagtagg
     2521 acactcaaat ataagtacta ctctaagcgt ttattctcat gtacttaaag aaatggataa
     2581 ggaggcttct gacaaaattt ctgaaagttt atttaatgct aattaatatt aatttatctt
     2641 agctttaaat tagcttagct tattgagata gattcattaa tattaaatgt atctatctca
     2701 aattaataac tacttaaata atctgtttta ttttaattta tcgcctcagt tattatactt
     2761 ttttacgaaa aattatactt tacaataaaa aagatattcc cttatagaaa atatcttttt
     2821 aaatactata ttattatata cttgctaaca ttggaatatc taacatcttc ttcattacat
     2881 catctacttt ttttgcttta tattcattgt atccaattaa atatcctttt tcatatgaac
     2941 tttttaaaat ctctttttca tataaattat cagatttggc ttctgtgaca cttaaattat
     3001 aaagcccttt acacccatct tcataacctt tattataccc tttttctgca aaatagtttt
     3061 tatgattttt ttcgttatgt actgaagata ttgttactcc agaattttct tttgtactag
     3121 cataaggaca aaaaccttta ttgtgttgat gtgctgaatg tcctccacaa taataacgtg
     3181 ttaactctga tttattttta ttatgatgat ctcctgatga attatgccct cctgagtgag
     3241 catatgaaaa attccctaca ctaatcatag ctaaactaat tactacagct actaaccttt
     3301 tcttaaccat aatctattcc ccccaaaatg ttatatattt ttattatatc atctactata
     3361 ttatatttaa attcttaata tacaaaatat tactctattc tgctaataaa catgtttaaa
     3421 caacataaaa aacctcctaa tatttgatta aaaatttaca cactcatgca tagcatgtct
     3481 caaatattaa aaggttttat actaataatt atttatcttt tccatacatt ttagctaatt
     3541 ggtcttgtat ctctttactt tctaaatact catcaaagtc catttttcta tccattattc
     3601 catttggagt tatctctata attctatttg ctatagtaga tataaattca tggtcatgag
     3661 aagtaaataa aagcactcct gggaactttt caagtccttt atttacagaa gttatacttt
     3721 ctaggtctaa gtggttggta gggtcatcta atacaagtac atttgcattt gaaagcatta
     3781 gcttactaag catacatcta accttttctc ctccagatat aacttgagct tcttttaatg
     3841 cttcttcacc tgagaataac attctaccta aaaatcctct tataaaactt tcactttttt
     3901 cttcagagaa ctgtctaagc cagtcaacca aagaatattc tactccgtca aaaaacttat
     3961 tatgattttt tggaagataa tcttgagaag tagttactcc ccatttatac tctccactgt
     4021 ctggttcaag ttctcccata actatgttga aaagtgcagt agtagctatt tcatctccca
     4081 taaatactac tttatcatct ctatccaatc taaatgacac attatcaaga accttaaccc
     4141 catctatagt tttagttaaa ttgtgtactt ctaaaacttc attcccaatt tctcttgcag
     4201 gtgtaaatcc tacatatgga tatcttcttc ttgatggttg tatatcttca acttctaatt
     4261 tctctaactg tttctttctt gatgttgctt gtttagcctt tgaagcatta gaactaaatc
     4321 tagctataaa ttcctttaat tgagctatct tttcttcagt ctttttattt tggtccttag
     4381 ccagttgtaa agccaattga cttgactcat accagaaatc atagtttcca acgtacattt
     4441 gtattttacc aaaatctacg tcaactatat tagtacatat ttgatttagg aaatgtctat
     4501 catgtgatac tattataact atagattctt ctaaatccat tataaaatta tttaaccagt
     4561 ttattgattt aaaatctaag tggtttgtag gttcgtccat taatagtatt tcaggcttac
     4621 caaaaagtga ttgtgctaac aataccttaa ccttttcccc accaactagt tccttcattt
     4681 gtttgtaatg catatctttc gttatgccaa gacccataag tattttctca gcattagttt
     4741 ctgcatccca tccgtcaagc tctgcaaact caccttcaag ttcggcagct tttatcccat
     4801 cttcttcatt aaagtcttcc ttcatataca atgcatcttt ttctttcatt atgttccaaa
     4861 gtctctcatg acctcttata acaacattta aaacagtttc ttcttcatat tcaaagtggt
     4921 cctgttttaa aactgacatt ctttctttat cagttataga tatactacct gtattaggct
     4981 ctatttctcc tgataatatt tttaaaaaag tagattttcc cgcaccatta gctcctatta
     5041 ttccatagca attaccttta gtgaatttta agttaacatc tttaaatagt tccttatcac
     5101 caaatctaag tccaacacct gtaacttgta acattaatat attttccttt ctttttgctc
     5161 ataatttatt ttatcataaa actttgataa catttttatt atatattatc acgtaaatag
     5221 tatatttttt atactaaatt tgtgaaatat ttattaaaat aatactaaaa attgtaaatt
     5281 tataaaaatt aacaatctct tatattatac ttataaagaa atcaaattgc aactttattc
     5341 attcttttac acattgaaaa taagttatta taatttctaa ataaatatgc ttttcttaaa
     5401 acatcattct agctgttttg tttcatattt aaaaagagta cttaatttgt aaatactctt
     5461 ttttaataac tttatatttt agcaaaatta catgcaggat aagtacatac atcacagttc
     5521 atacaaaggc caccatgacc atatctagct atatcatact tatcaagctt ttcatcaatt
     5581 aaaaccctag ataaaactat atcaaatact gtagttttgt gatacattgc gcatcctggt
     5641 atacccataa ttggaacttc tccataataa gcaagtaaaa acatagctcc tggtaatatt
     5701 ggtgaaccat atgtcactaa atcagctcca gtttctttta tagctgttgg tgtaacatca
     5761 tctgggtcta cactcatccc accagtacaa cagataagtt ctgctccttg acttataaat
     5821 tcttttattg cactctttat aatttcttta tcatctgggc atatagtctg tcctataact
     5881 tcacaaccaa agccttttac tttttcttct attacgggtc caaatttatc aacaattctg
     5941 ctataaaaca cttcatttcc tgtagtaaca attcctactt tcttaggttt aaaaggtact
     6001 acatttacta tttttctatt accaactact ttttttgcat cttctaattt tttctcatct
     6061 ataactaaag gtatcactct tgttccagct acttttaaac ctttatttac tataaaatta
     6121 ttatgaagag tagccatcat tatttctcca acacaattca aatcaaaaag tgcatcaaca
     6181 tctatcttta atagaccatc acatgctgct ataaaatcta ttttcccttc ttttatttca
     6241 ctaaaatcta aattttctcc agcagttagt tcttttaaaa acaatgcgcc ttcattttca
     6301 tgaaccattc cttctgactt ttcccataca tacaaattat ctttaccaag agataataac
     6361 actggaatat cttcttcttt tactatgtgg ccctttttaa attttcttcc tttaaattct
     6421 ccaggtatta tttgagttat gtcgtgacat aaaacttgtc caacagcatc tatagttttt
     6481 attagcttca taatttaccc ccaattctct cataatccat ttttgtatcg atatcaaata
     6541 acatttcttt aggaacttct acatacttta tttttgatga attttttaaa atggttttac
     6601 ctttttcatc ttctttaagt gccaacaatt cttctttctt actatatggg aatatgactg
     6661 gatttccaca catatctttg tatcttggaa ttactataaa atctctatct aatttaaaag
     6721 tatttataat tttatctata tataagttat caataaatgg ctggtctcct acaaagaaca
     6781 tataaccatc acattttgag ctatttaaaa ctcctaattt tatagattca ctttgaccta
     6841 tatttgcatt tttattatct atatattcaa aaccatattt attacacaat aacaaaacct
     6901 cttcatattg acttactact acaacttgat gaaaatcaat cttgctaact tctctaaata
     6961 catgttctat tatagaacag cctttataat ttaataaaag tttatttgct cccattcttc
     7021 tggcaaagcc tgatgccatt atcactgcat ttatcatata tatcatcctt ggtatttata
     7081 atatattgat agttctatga tttttataat atctctattg atattatcta tatatataat
     7141 atcatataaa ctattttaat acttcaatat cttacacaaa atttttgttt tacacttcca
     7201 taaaatatct ctatttcact ttcatattct ttaatatttt ctactaattt tatagctaaa
     7261 tttttatatt tttcattttc aactttatta ataaataata ctctttttcc agaataattt
     7321 ttaaataatc catttggatt taatactata ttttttaagt tttcaatact tactgtagaa
     7381 ctactattaa tactacttgt atctaaattt gctatttttt taaatatttc tactctatgt
     7441 atattctctt cattaatatt cattccaaat gatgttatat ctaatatccc tatggttttt
     7501 gtagttttat gatatactac tggttcttta gcattccaac ctttaagttt ttttctcttt
     7561 gagccatctc cctctattaa aactaaatca aattggtcta caatttcatc caaaatagag
     7621 gaatcaatac ttactaattt attttcatta tttatataac tgccacagac tgttattcca
     7681 tgacaaatag atggaataac agttccatct attgtaataa ttatattgtc ataatcgttt
     7741 ggtacatata ttttagttgt tgttgtaagg agaactttca atttatctct ataaaaattt
     7801 gcaaaatagt ttataaacga agtttttcct cctgctccaa ctacagttat tatttcattc
     7861 ttttctatta tatttgatag ttccacactt tcacctctta taaccagttt tctaatctct
     7921 taattattat agttagacct ttttagtaaa agtgattttt gaaattccat aaactcgaaa
     7981 gtaaatagat tctgttgtaa acaacaaggc ggatccaatt cgccgctaag ctgtaagctt
     8041 acgctatcag tttatctttt aaatataatt aagatattct tactctctgt ggttgcttta
     8101 atatttgagg attattctgt aaattagata aattaactac ttgtgatttt tttagtatat
     8161 taagactcgc atttacatct gcatttataa tgattccaac actggatttg tactgtccac
     8221 gcgttattct ttccccttta aagtcatatt ttttaacatt gtccacattg atatcaggta
     8281 gaaaatcatc agcaaaaaaa tctgatttac tagtatagct ttcttcttgc ctaataaaat
     8341 tgatattttt tattttacac agatattcta attttccttt tatctctcca aatgggattc
     8401 ttataaaatt tctatatatt tttctctcca tattatcact tttttctaac ttagcattat
     8461 atcctacaat taaatttcca atattatttt ttacacaata atcaattata taaaaacagg
     8521 ttttatttac atagtcttta acttgattat ttctcttatt ccaaacttta aattgttttc
     8581 ttgttattgc agttatattc ttttttaatt ttaaggcttc tattttatca ttctgtttgt
     8641 ttgcccactg attcacagat tttagccttt ttccatctat gataaaacta tcacctaaat
     8701 tggtaacaca tgtacataaa ttttctaaac ccaagtctat agctagtgca tttttttgat
     8761 ttgaaacttc ctctgatttt ggtatttcat aacaatactg aatttcaaag aacctagctt
     8821 tatgtttagg tattattctt atttctttaa tttttttctc tagtatattt tgtggaactt
     8881 ttatacttac tttcccatat agttttttaa atgctggaga cataggtaca tttaaaatcc
     8941 catcttcttt gattcttatt tgacctatta ttatgctaaa aaatccttct ttatctaaat
     9001 atttaggtga ttttatccct ctaaagtcat attttccttc tttggctaat ttaattaatc
     9061 caaaaaatga cctaaacgaa ctgtctactt cttttaatat ttgttgcgct atattactat
     9121 tgagaagctt atagttctca ttttctttgc atacatgata attagcttca tagttaagat
     9181 gttctccttg attgaaataa tgctgtctta cattatatgt accaacatta tataagttct
     9241 tagctaagaa gcacatctct cgtagagctt gatattcttt tttagttaag ttttttaatt
     9301 gttgtctctg agttccatag tgtatatttt ttattctgtt tgacatgaac tcacctcctt
     9361 tagaatatac tctactagac attatatacg gtatttttac aaaacaccat atataatgtc
     9421 catttaatta ctttatttta caaaataatt taaatttact tgcttttata cacattatta
     9481 tttattataa ttgtttatta ttatttttca tacccatata aactttttca aaagtcatag
     9541 gaagctctct taaacgaact cctgtagcat taaatattgc atttgctata gctggtgctg
     9601 gtgtatgtat aactatttct cctaatgatt tagcaccata aggacctcct gggtcgaaat
     9661 tctcagcaaa atctaccaat atatttccaa cttctttttt tgtaggaact ttatactgta
     9721 ggaaactatc tgttaataat ctaccatctt tatcaaatat agggtcttca tacattgcaa
     9781 accctatacc catcatcaca ccaccttcta cttgtattct agctaaagtt ggatttataa
     9841 ctgttccaca atcaggtaca catgcaaaat ttatgacttt atattcgcct gtatttttat
     9901 ctaattctat ctctgcaaat cctgctataa atggttttat ttttttcaac atgccaaaag
     9961 attctgttgc ttccaaaact actggtacat catcactagc aaatcctatt cctccaacag
    10021 attctctacc caattcctct aatagaacaa acttgctatt atcgttttta acaaaaaccc
    10081 tgtcttttcc caactctaaa tcttcttttt taaatcctag ctttgactct gcaactgaaa
    10141 gtaatatatc ttttaattta tttgctgtct ttattgttgc atttcctgta accgttgtcg
    10201 tacaagaagc aaatgctcct gtatcatagg gacataagtc tgaatcacct gtataaatag
    10261 aaattctatc tacagtagta tttaaagctt ccgcagcaat ttgagcttga attgtatcag
    10321 ccccttgacc taagtcagca agaccagata atagtttata tgtgccatct tcatccattc
    10381 ttatagtgac tgttgccata tctacacgcg gtataccaga accatgattt gctactgcca
    10441 tacctactgc tcttactttg ttgtttccta tatctttaca tggatattta tcatcccaat
    10501 taattaattt cttgcccttt tctatacatt gtctcagctc acaacttaat atttcgtctt
    10561 cagctgcttt tccattggta tacctatcta ttatattttt aattctaaat tcaagtggat
    10621 ccattccaat cttatgtgcc aattcatcaa tagcactatc tattgcaaat attccctgtg
    10681 tagcaccata ccctctaagt gctcctgctg gtaacctatt agtatatact gttctagcat
    10741 taaatctaac agcatcaata tcattatata ttggtaaggt attgtgtcca gattcggaag
    10801 aaacagagac accattccca ccataagcac ctgtattgtt taatgacttt agctctattg
    10861 cttttaaatt tccttcttta tctgcaccaa ctttaacatc aaaaaacatt tcatgtctac
    10921 tattagaacc tacaaaagtt tcacttcttg taaatacaaa ttttgctggt ctaccagttt
    10981 tcatagtaac aaatgcagta aatatatctg ttgctgctat attctttcct ccaaatccac
    11041 cacctattct aggtttaatt actcttattt ttgaagatgg tatacccaat acttttctaa
    11101 gtattctctt tacattgtgt ggtgattgta atgatgaaac tacaacaatt ctatcattta
    11161 tatctttata tgaatatgac ctatgagttt ccatcatgca atgcgattga ctttgggttt
    11221 tgtatgaaga ttcaaccaca aaatcacttt ctttaagctt agcttcaaca tctccaaatt
    11281 caaaaaattc ttttgatact atattatttt ttgcatccaa tccaaaagga tatggctcta
    11341 ctatgtcttt ttctggatgt ataacaactt catttccttc tgcttctctt gcatcaaaca
    11401 caggctttaa aacctcataa tctaccttta tcatttttaa ggctttaagt gccgttttct
    11461 catcttcagc agccactaaa gctacttcat ctccaataaa tctaactgta tcttcaagta
    11521 aaactctatc ataaggtgat gcttctggtt ctgtttgacc tggcacacaa tatctgacct
    11581 gtggtacatc tttatacgta taaataccca caacccctgg aattttttcg gcttttgatg
    11641 tatctatatt ctttatctta gcatgagcat gtggacttct aagtagtttt aatgtcaaaa
    11701 catctttatg cataatgagg tcttcagtat atagaggttt tcctgtgaca attgctgtac
    11761 tatctatctt tcttatcccc ttattaatca ttatacattc cctcctcaat agcattatac
    11821 atccttttaa ctaatgcact tgccatatcc tttctgtact ctttactagc tctcatatta
    11881 cttccataag taagttcctt actagcaaca atagatgcct tatctatatc tttttcttta
    11941 tttaatatat tactcgcttc aagtgctaat ctagcttttt gaggtcttgc cccaattgct
    12001 attttgtaag tatcattttc ttttagcatt gctgaattta atacagaaaa atctcctgtg
    12061 cattttctta ttgagtcaaa aacagctatt gcatcttttt ttggtagtat tacttcgacc
    12121 aatatatccc tacttaattc actatccaaa aactcactta actctagtat tcctttattg
    12181 tataatctca ccttcgctcc aacaaccaaa agcgctggta ttaagtcaga aaagccatac
    12241 tttgaaaata cacttgcacc aattctagcc ccttgtctaa attgaacacc aactatatta
    12301 gaaacagcgc ttgaaagtat tccactacag taatttttta tagttttatc aatttctaaa
    12361 cttctaagtg aagtatctgc acctatcaaa atattttttt tatcctcttt tatataattt
    12421 agatttatat cttttaaatc tatcgctgtt cctatatttt tattacttaa ttttataaat
    12481 ccacatccac ctaaaataac attatttttt ttagaaatta aaattttata tgcttcttct
    12541 aaactctgtg gaactgaata ttcctttata gtaaccatct tatcccccca tcattaactt
    12601 attaaaacac aaaagttttt aataaatctt ttatttatat ttataattgt agtatattta
    12661 attcacatat attaaacctt tacattataa acttttattt ttatcctcaa ccttatcact
    12721 tttatttcca ggaaatttta ccatatataa agcttgaatt attatggcta ccattgctcc
    12781 tgaaactaca cttgaatctg caagtaatgt ctctataaat tttggaaatt tttcaagaac
    12841 ttgaggagct accatgaatc caataccaga agccatagaa agacctaata tcatgctatt
    12901 ttcttgatta aatccatcca tactaacaag tcttaaacca gatgttgtaa gagttgaaaa
    12961 tataacgagt gttgctcctc caaccacaca gggaggaatt gttcttatta aattagctaa
    13021 taaaggcgat attcctgcca aaaataatcc aattcctcca atagctagta caaatttact
    13081 tacaaccttt gtattggaaa cgattgcaga attttgtcca aacatcccca ttggagttgc
    13141 agaaaataat gcacctattg tagagagtac tccatttcct aaaacagcag aagaaagttc
    13201 ttcatctgtt acttgtctat taagccctcc taccgcagaa agtgtacaag cacccattat
    13261 atcagctatc tcaactaaat atattattga aaacattaca acaacttcaa gtcttatatc
    13321 tagcccaaaa gcaagtggtt ttggaagtgc aaccaaagta aactcctgaa ttgaagaaaa
    13381 atttactata cccaaaatta atgataaaat gtatccaaaa attatgctaa ctaatattga
    13441 tgcatcttta aataatcctt ttccatactt atttaatcct aatatcataa gagatacaga
    13501 tattccaact ataaaattaa ttggattccc aaaagtctca ttgcccatac ccccagctaa
    13561 gttttttata gctattggaa aaagtccaag acccatacat gcaactattg ttcctgatac
    13621 agctggagta aatataaatc gtattttttt aagaccaaaa ccaattataa ttattactat
    13681 accagctatt atttgagagc caaataatac tggtagtcca tacttacttg caacagatac
    13741 cccagctcct ataaacatag ctgtagctcc cattataact ggtattcctc ctcctaattt
    13801 aaaaccttta aacaaaggta ttggatataa ttgtaataaa gttgataatc ctgcaaaaac
    13861 taatgcggct tgaatcaata atgttgacat cttatgatta agtccagctg catttgcaat
    13921 caatatcgct ggtaaacaac tacctattac cattgccatt aagtgttgta accctaaagg
    13981 tattgcttcc tttagtggtg gtattccgtc taattcaaat tttgatgtac tttttgcttt
    14041 cataataaaa actcccgtta cataaaactt attgaattat ataaatactt aaaaatctaa
    14101 tatattaaat taatgatgtt cataaatatc aactatactc caacatatat ttaagttttt
    14161 agtcttaaaa aacagatgca ataaatttaa ttattaacaa ataaacgtat tctttaattt
    14221 tgtatacatt atataccatg ctttacaaat ataagagtat tatagctatg aatttttaat
    14281 tttttatagt aaatttaatt caatttaaat ctttcaatct taatcattat aagttataag
    14341 ttttcataag tatttacatg tttcaagttt taaacattta cctataaacg ctactttata
    14401 gatgtcactt caaatccagt gacctcatac tttcatatga gcgtagacta tttcttcatc
    14461 ttcagcatta cctgctaaga gcaacccgtt tccactatca atcgcttata gtgtactctc
    14521 tctcgagata gtcgtttgac cttcctattt ctaggcttgg cgaccaaatg tccattttta
    14581 agtacttagg ctctcaccat ataccatcct cactattgtt tctggttttc accacattca
    14641 taaaagtttc tttctatctt ttattgtagt tgcaaggctt taggatttac tggttttaaa
    14701 gttgtgtcct acatagattt ctctatgtac ggggccaaaa gtacaattta tttaacatac
    14761 tttgctttac cccctgtaaa taatattatt tactctttca ctgtttttgt tattaaatta
    14821 attaaatatc aattaatcta agcataaaat aattatttaa tttaaactca aaatatttat
    14881 gaatatttta taatataatt ctcataagac agaaaaactt aataagctat gtatctcagt
    14941 tgtaccaatg tactctcctt tactatattt gtatctgcaa cataaaatat acaagtttct
    15001 atctatttat agatatatat atctataagc aaaatagtaa tattattttt aatagtaaaa
    15061 attttattca tattactatt tataattttt atagagcaat tttagtgcca atatttgcat
    15121 acatttgtat agtataaaat ttttatgttc tcattgataa aattttactt atttcaagcg
    15181 ttctattaga ttttatcatc taaattttag gttttatttt tataaattaa ttgtatttac
    15241 ataaaaaaaa agacctaaaa acaaattttt aacggtcttt tctcaatttt ttattttcat
    15301 ttctaaaatt tataataaat agtaagaaca ctataaattc ttttatataa aattcttagt
    15361 ttgtggatga taaattagtg aattaaaata ctactttact gattttagtt tgacaatatc
    15421 actccaattt ttactagtgg ctaactctac taaatttaag tctcttctta tttcagctat
    15481 atcctctttt atagaattag tgtcaccttt tatatatgct atgttatgtt ctatattatc
    15541 atgagtagct ttatttattt cagaattatg taataaactt cctaaaatat tattctgttc
    15601 tatctgtatc tctttcattg atttatcttc acattctaga ctatcaatct taatttcaat
    15661 tccatataat ttttcatttg ctatggatac ctcgtcctta attccattca tatcttcatt
    15721 aatgttattt attttatttt gcataaagtt tacttctgat ttaacttcgg aaacatcttc
    15781 tttcattaca gttatttctt gtctcacttc tgacatgtct tttttcatta catttatctc
    15841 ttgttttacc tctgatgtat cttctttcat tacagtcatt tcttgtctta cttcggacat
    15901 atcttctttc attacagtca tttcttgtct tacttccgac atatcctctt tcattgtatt
    15961 tatctcttgt cttacctcag acatatcctc tttcattata tttatctctt gtcttacctc
    16021 ggacatatct tctttcatta cagtcatttc ctgttttact tcggacatat ctacttttaa
    16081 tacatttatt tcctgtttta cttcggacat atcttctttc attacagtta tttcttgttt
    16141 tacttccgac atatcttctt tcattacagt catttcttgt tttacttccg acatatctcc
    16201 ttttaataca tttatttcct gttttacttc cgacatatct tctttcatta cagtcatttc
    16261 ttgttttact tccgacatat cttcttttaa cacatttatt tcttgtttta cttccgacat
    16321 atcatttttt atgctaacta catcttcatt tatagtatat aataactcta aaatcttctc
    16381 ttgcacttgc atcaccatcc ctattaatta ttataacatt ttacacaaat gctatacaat
    16441 gttaacatat atatcagaat tattaaaatt ttaattaaac aaaattgatt cactttatta
    16501 cttcaaaact aattaatatc taaaataact acaaataaac aaaaataagg tcaacaacaa
    16561 aaaaggctta tctctttgag atagcctttc ttatttttat ttaaaacttt gttttttata
    16621 aaataatcta tacattcagc tctgaacatg aaatttttct tccaagtgct tttggtttac
    16681 aaactgatat acaaagtcca caatatctac actcttcttc attcaattcc atcttatcat
    16741 tcaattttct cgcttgatat gcacaaactc tctcgcacat cttacattta gtacattttt
    16801 cttcatcata agtaaactca aagctaagtc tttcattaac atctcccata tgattatgta
    16861 ctataccaca agcattttct atagtattat atcctaatct attaagatta gattttaaat
    16921 catcatgtat tttagatatt acttgtgcgc cttttaaaat tactactgaa catataccaa
    16981 caaagtcagc acctgccata atcatttcta aggcatcatc tccacttaca catccaccta
    17041 agccaattat tggtatatta acttgttttt ttatttcata tattctctgt aaagttattg
    17101 gcttgatagt ttctccactt aaatatccta ttccatttcc acctaataaa ggttgccctg
    17161 tttctatgtc tattctaaat gctggtccta cagaatcaca agcagttatt gcatctgctc
    17221 ctgcttcttc taatttttta gcaaaatcgc ctatctcatc tatcatagga ggaagtttaa
    17281 ctattacagg tatatttact ctttcctttg catctttaag cattggtatc aaatctctat
    17341 agtcataaga tactaattca ataaagtcag ctcctgcatt tgcaactttc tctactaatt
    17401 cgctactttc ttcaattgta tgacctactg aagctataca gacagtccca tctcttttca
    17461 tttgtggtat ttcaaaatct atccattgct ctggctcaaa atctgtccat ttttcattat
    17521 ttaaaagagc attagctgta tttctaacca tatgtggtgc tggatttata gctctttctt
    17581 ttcttatagt cttagtcaca actgcccctg ctcctgcatc acttaatatc ttgcatccag
    17641 ctgcactata tgttaatggt cctgaaccta ttataagtgg tgattttaat tctttaccta
    17701 aaaaattata cattatctgc ttcccctttt catatatatt tacattcgtt aaaatttcca
    17761 aaattcacaa gcaacttctt tcatttcttt aacaatagct tttttatcta aatcaactaa
    17821 ctgtttttct tttaaaatat gtctaccatc tatgtataca tttgatataa attctttttt
    17881 accctgaact actatctggt caaatatatt atcaagagtc acaggagttt taaattcatc
    17941 ttccataact attatatcag ccttattacc aactgctatt ttaccagaat tttgaagtcc
    18001 taatacataa gcaccatgct ctgttgccat tctaaatact aaatttgctg gcattactga
    18061 tgcatcttct tcaactgatt tatgcattaa aaatgcacct ctcataactt caaaaaaatc
    18121 atttatatat ccgtcagtac ccagtgccac ttttatacct tttttaatca tttttggtac
    18181 tggagaaaaa cctccaccta cttcacagtt gctaagaggc atatgaacca cttttacacc
    18241 tttttctttt aatatatcta tttcttcatc atttactttt acacactgag atgctaatac
    18301 agtttcatcc aatatatcta aatcattata gtagtcaact gcttttttgc caaaatgttt
    18361 ttcagcatag tttggctcat atatactctc acataaatga aattgcattg gtgcatttaa
    18421 tttttttgta tcttctttag ctttcttaat aaatctatca gaacatgtga agcttgtatg
    18481 tgtacacatt attccattta ttaatttact attttctcta ctccacttta ttaaattaga
    18541 attttcattt aaacatctaa gtccattttc aaagcttatt ctttcacaac tttctaagga
    18601 taaaatcgct ttcatgccta tttcttctag tatttttcct tgctcaatta aagttccttc
    18661 ttctgtattt ggagcttcta gggtatcaca aaaagctacc acaccagatt ctataagttc
    18721 tattgctgat gctttagttg ttgccttcac ttctttcaat ccaattctat tttctataaa
    18781 tggccaccaa tagtcattta agaaaccttc aaagtcggta aaatgtacat ttgccggtat
    18841 tcccctagaa agaactccat attgatgcat atgaccattg acgaatcctg gcattattat
    18901 agcatctgat ttatctatta tttcttcaat atttttatat tttatttcaa cttcttcatt
    18961 tggcaatata tcttttataa tgtcattttc tactacaaca gcagaatttg tatatatttc
    19021 atcagcagaa gatattaagt atttactttt aatagcaatc atttatttac ctccctgatt
    19081 taattttttc atggccatat aaactttctc agaagttata ggaagttctc ttatacgaac
    19141 tcctgtggca ttgtatattg catttgctat tgctggtgca ggtgtatgaa taactatctc
    19201 tcctaaagat ttagctccat atggacctgt tggctcataa ttatctacaa aatcaacaat
    19261 tatgtttcct atatcttttt ttgttggaac tttgtattgt aatagattag aagtttgaag
    19321 ctttccatca gaaccatgtc tagggtcttc atacattgca agccctatac cttgagttac
    19381 accaccttca acttgtattc tagcaagtgc aggattcata actgtaccac aatctgtaga
    19441 acaagcataa tttattactt taaactcccc tgttgtttta tctaactcta tttcagcaaa
    19501 tcctgctaag aatggtttag cactctcttg tataccataa gattcagatg aatataaaac
    19561 ttcttgattt ccaccaccaa cagattcttt tcctaattca cttaacaaaa caaacttctc
    19621 tacatcttca ctataacaaa ctctgtcttc atatagtatt aaatcatcta ttggtaaatc
    19681 gagtttcttc tgtgctactt ttaatatctt ttgttttaaa ctttcagcag attttattgt
    19741 agcatttcca gttacataag tagtacaaga agcgtatgca ccagtatcat atggacacat
    19801 atctgtatct cctgtataaa cggaaattct atcaatagat gtattcaatg cttttgcagc
    19861 tatttgtgca agtattgtgt cagaaccagt tcctaagtct gaagacccag aaaacaactt
    19921 ataagttcca tcttcatcca tcctcatatt tactattgcc atgtctatgt tggctatacc
    19981 agaaccatga gttcccacag acattccaac agccctcacc ttattgtttc caatatccct
    20041 aacagggtac ttttcatccc atccaataag ttttttacct ctaattatac attcatcaag
    20101 tgcacaactt cttattggaa aattcattat tccgccttca gtttgttttt ttataatatt
    20161 ctttaacttt atttctgttg ggtccatatt aagtttatgc gctaattcat caattgcaca
    20221 atcaagtgca aatgtacctt gtgttgctcc atatcctctt aatgctcctc ctggaactaa
    20281 attagtatat acagttctcc catcaaattt tattgcaggt acattattat atgttggaag
    20341 tacattatga cctgattcag agcaaactga aggtccatta tcgccataag cgcctgtatt
    20401 gttcaatgct ttcatatcta tagcttttat gtttccttct ttatctgacc caatttttat
    20461 atcaaaaaac atctgatgtc tagtattagt cattgcaaaa gtttcttctc tagtataaat
    20521 tagttttgct ggtctttttg tcatccaagt tacaaatgat gcatatatct ctgttacagc
    20581 tatgtttttt ccaccaaaac ctccacctat tcttggtttt attactctca cttttgaaag
    20641 aggtattccc agtgctttag aaacctgtct tctcatatga taagctgatt ggtttgcaga
    20701 agttactact aatctgccat tcgtatctat atacgtataa gctctatgag tttccatcat
    20761 acaatgggct tgtgattgag tcatataaga atgttctact attacatcac tttctttaaa
    20821 ctctttttct acatcccctt tacccatttt ttctctcgat acaatatttt ttgtagcatc
    20881 caatccaaaa tcaaatggac aaaacaaatc atcttcttca tggattaaaa ttttatttcc
    20941 ttccgattct cttgcatcca atataggagt cattacttca tattcaactt ttataagttt
    21001 catagcttta atagcagccc tttcatcttc agcggctatt atagcaactt catctcctac
    21061 atatctaact acgtcttcaa gtattaattg gtcatgaggt gatgcttctg gatatgactc
    21121 tccaaccatt gaatatctat aatttggaac atctttgtaa gtgaatatgt ttactacacc
    21181 ttttaccttt aatgcatttt ttatatctat gttttttatc ttcgcaaaag catgtggact
    21241 tctaagaagc ttaattgtta aaacatcttt atgaaaaatt aaatcttctg tatatattgg
    21301 ttttccagta acaatggctt cactatctat ttttcttacc tctttattta ccatcacact
    21361 cacctccaat actattatac attctcacta ctaaggcttt tgccatatct tttctgtatt
    21421 ctttacttcc tcttatatta cttccaaatg aaagttcttc acttgatact tcaccagctt
    21481 tttctatatc attttctaaa cttaatattt cacttgcttt ataggcaatt tttgctcttc
    21541 ttggtctagc gcctatcgca attttataaa tacttccttc ttttaacatt gctccattta
    21601 atactgcaaa atcacctgta cattttctta tagaatcaaa tacacctata gcatcttttt
    21661 taggtaatat aacttctatt aatatatctt tttcaagctc attttctaaa aaatcagcta
    21721 agttcataac ccctttttta tataacttaa cctttgcatc taccactagt aatgaaggta
    21781 ttaaatctga aaatccatac ttagcaaata cgctagctcc tactctagct ccacttctaa
    21841 attgaactcc tactatatta gaaacagcgt ttgaaattac cccattacag taatttttaa
    21901 taaccttatt taattctaaa gttcttaaac ttgtatctgc tccaattaat atgctatcat
    21961 cagtttcatt tatataatct aaagctaagt cttttaaatc tattgctgaa ccaatatttt
    22021 tactacctag ttttaaaaat ccacatcctc caagtattat gttgtttttt cttgatatta
    22081 gcagttcata agcttcatct aaactttttg gaactacata ttcctttata gttatcatac
    22141 tctctcccca tatcaactta attttcactt aaaagtctaa gtgtttaaat atttatgtat
    22201 tgctcttaat tgacctttat atcctgtaca tctacaaagg ttaccattta cataatctat
    22261 aacttcttct tctgttggat tttgaagctc tcttttcatt gccaagacca tcattataaa
    22321 tccaggagca caaaatccac actggtcagc cccttcgtct gcaagaaaat ctccaaactt
    22381 ctttgcttca tccaagacac cctcaagtgt tgtaatatgc tgaccttcta ctctagcagt
    22441 aagataatta catgaaagca cggccttgtc atttaccatc actgtacata acccacatga
    22501 gcctgtatca cacccttttt taacacttac atatccattt tctcttaatg tatctgctaa
    22561 aaaatcatct ggtcttatac ttaattcttt ttctttatta tttatggtac atttgataat
    22621 caaataaatc acctcaaaac tcatattttt aagctaaagt tttactatac ttttttatga
    22681 ataaattaca tttattaata aaggctattc catttatgac cttccattct attttttata
    22741 gttcctctta cacaaagtct ctcagctgta tcttgagcat cttttaatgc tttctcttca
    22801 ttagctgtca atatttttga atcttccatt attatatttc catctactat aacactctct
    22861 atatttttca tactagaaga atatactaat gtagataccg gattatgcat aggtatcgct
    22921 tttggtgaaa gcattggatt aaatattaat aagtctgctt ttttacctat ctccaatgaa
    22981 cctatctcat cttccattcc aatagctctt gctccatcta tagttgccaa ctcaagaact
    23041 ttttctgctg acattgctaa tgggtcacac ttattaactt tatgttgaag tgctgttaat
    23101 ttcataagtt caagcatatc ttgagagtta ttactagcgg ctccatctac tcctaaacta
    23161 actgttattc cttttttaag catttctggc actggtgcaa cacctgaaga taagtacata
    23221 ttacttgctg tattatgaga cactttcata tcgtattttt tagtaagttc catatctttt
    23281 tcagttagat acacacaatg taccataagc acatttggtc caagtattcc taatttttct
    23341 aaaacatcta tgtcatattg cccatgcaat tcttttgctg cttctctatc aaaaggagtc
    23401 tctgatatat gtactgtaaa taaagcatca tcatattctt ttactactct ccatagcatt
    23461 tcaagcattt cttgagaatt tgaccatatt gctgcaggag ctactcctat tttgattctt
    23521 ccattttctg tattatggtg tttttcaaac aatcttctta catctttttc aaccgtttct
    23581 acatcttgca ttattccagg atgaactcca aattgagctc ctgtattcat acatcctctt
    23641 ccaagtattc ctcttattcc taattcttta taagcatcta ttattccatc acaaagacct
    23701 ggcttactat gaggatacat gtagtcaacc attgttgtta taccacttct aagtccttca
    23761 atacatccaa gcattgcagc atcataagta tcttttggct ctaaatagtt agctgctgga
    23821 aatgtcatag tttctaacca gtctttaagt accatatcat ctcccaatcc ttttaataaa
    23881 gtttggaata aatgattatg tgtatttata aaacctggga ataaaacttt acctttagca
    23941 tctataattt ttttaacatc agtatattta ctttctatct ctttagaatt tccaatatca
    24001 gcaatcttgt tatcttttac tactaatgcc ccatcaaaaa taacttctct ctctttattc
    24061 acagttacta ttatggcatt cttaataagt atatccatca ttcttatttc cccccgcatt
    24121 atttagtaat ttttctttta atatattgac ctgttggatg ttctttaaca ttacaacttt
    24181 gtactacatg atttcctctc aaaaatacat cttcaacttt acatttaacc ttaaatcctt
    24241 catatggagt ataatctaca ttttgttttt gagttttata actaattgtt tcttctttat
    24301 tgatatttaa aatgactata tctgcatcac ttcctacagc tatctcacct tttttaggat
    24361 acattccaaa tatttttgca gcattcgtag aagtaacttc cacaaactta tttgcactta
    24421 ttctattttc taagactcca tatgtatata aaagtgctaa cctatgttca acaccaggtg
    24481 ctccatttgg tatcttacta aaatcatcaa ttcccaagtc tttttgcccc ttaaaattaa
    24541 atgaacagtg atctgtgcca actgtttgaa tatctccact agacaatcct tcccaaaggt
    24601 aatttatgtc ttcttttttt cttaaaggag gagacattac atacttagca ccctcaaatt
    24661 tatccttatt atataaattg tcttctaata atagatactg tgggcatgtt tctactatca
    24721 tatctatatt tttttctcta gccattttta cagtttcaag tgactcccta caacttaagt
    24781 ggactacata ggattttgaa ttagataatt tagttatatc agcaagtctt gaaacagact
    24841 ctttctcaac tatgttaggt cttgtaagtg gatgatattt tggctccaaa ttaccatttt
    24901 gtatattttc ttctatcaat acatctagta aatccccatt ttcacaatga aatccaacta
    24961 tacaacctaa atcccttgct ttctttaaaa ccctgtatat tgttccatca tctactttca
    25021 tgccatcata tgccatatac atcttaaaag atactatccc atccgaaata agtttttcca
    25081 tatgattaaa tgtatcttta tttaaacatg taattgtcat atgaaaacca taatcacaat
    25141 aacaatttcc acttgctttt ttatgatatg cttctacacc ttgcagtagg ttttccccct
    25201 cattggcttc tgcaaaatct agtattgtag tagttcctcc agctatagct gctcttgttc
    25261 ctgtttcaaa attatctgca gttgtaatag aacctgcatt catatcaaaa tgagtatgtg
    25321 tgtctattcc accaggaatt acaattttac ctgtagcatc aataacctta tcaccattta
    25381 tttctaagtt atttcctatt tcaactatct cttcatcttc tatccttaaa tcacttatat
    25441 aagtcttatc agaggtaatt atagttccac ctcttagtat tgttcccata tatacctcct
    25501 aacttttaag ctttaaagta ttttctaagt attgtaaaac tttatttaaa attgtatttt
    25561 ctaaaggtgc tatattcagc acctctagac tatatttaaa taatttattg tgaactaaat
    25621 attattttcc gtatacagat tttaatattc catagtaacc ttctgttcct ataaataact
    25681 gttcttgctc tatatactca tctattgtgt gagcaagatt ttccttagaa ggaccaaatc
    25741 ctatagtttt tattccagct tcacctgcat agtgacttcc attcgtacag aatgagtatt
    25801 gagttatttc tgaatctatt ccagcttctt ttaatccctt atatgctgct tgaacaaatt
    25861 catcttcttc
//
LOCUS       DAMJWS010000058        25692 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMJWS010000058 DAMJWS010000000
VERSION     DAMJWS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.058"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..975)
                     /locus_tag="PC399_002441"
     CDS             complement(<1..975)
                     /locus_tag="PC399_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2593297.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDN"
     gene            complement(1023..1883)
                     /locus_tag="PC399_002442"
     CDS             complement(1023..1883)
                     /locus_tag="PC399_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2593298.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2152..2361
                     /locus_tag="PC399_002443"
     CDS             2152..2361
                     /locus_tag="PC399_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2593299.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2361..2627
                     /locus_tag="PC399_002444"
     CDS             2361..2627
                     /locus_tag="PC399_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2593300.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2656..3228
                     /locus_tag="PC399_002445"
     CDS             2656..3228
                     /locus_tag="PC399_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2593301.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3463..4653)
                     /locus_tag="PC399_002446"
     CDS             complement(3463..4653)
                     /locus_tag="PC399_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2593302.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4913..5344)
                     /gene="dut"
                     /locus_tag="PC399_002447"
     CDS             complement(4913..5344)
                     /gene="dut"
                     /locus_tag="PC399_002447"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF2593303.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5457..7685
                     /locus_tag="PC399_002448"
     CDS             5457..7685
                     /locus_tag="PC399_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF2593304.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7796..8281)
                     /locus_tag="PC399_002449"
     CDS             complement(7796..8281)
                     /locus_tag="PC399_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2593305.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8533..9756
                     /locus_tag="PC399_002450"
     CDS             8533..9756
                     /locus_tag="PC399_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2593306.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9760..12894
                     /locus_tag="PC399_002451"
     CDS             9760..12894
                     /locus_tag="PC399_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF2593307.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12928..14226
                     /locus_tag="PC399_002452"
     CDS             12928..14226
                     /locus_tag="PC399_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF2593308.1"
                     /translation="MSKIVAISCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14515..14760)
                     /locus_tag="PC399_002453"
     CDS             complement(14515..14760)
                     /locus_tag="PC399_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2593309.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(14974..17601)
                     /locus_tag="PC399_002454"
     CDS             complement(14974..17601)
                     /locus_tag="PC399_002454"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2593310.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17662..18504)
                     /locus_tag="PC399_002455"
     CDS             complement(17662..18504)
                     /locus_tag="PC399_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF2593311.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18511..19149)
                     /locus_tag="PC399_002456"
     CDS             complement(18511..19149)
                     /locus_tag="PC399_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2593312.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(19585..20877)
                     /locus_tag="PC399_002457"
     CDS             complement(19585..20877)
                     /locus_tag="PC399_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2593313.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(20914..21270)
                     /locus_tag="PC399_002458"
     CDS             complement(20914..21270)
                     /locus_tag="PC399_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2593314.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(21305..21664)
                     /locus_tag="PC399_002459"
     CDS             complement(21305..21664)
                     /locus_tag="PC399_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593315.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(21733..23706)
                     /locus_tag="PC399_002460"
     CDS             complement(21733..23706)
                     /locus_tag="PC399_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2593316.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(23928..24941)
                     /locus_tag="PC399_002461"
     CDS             complement(23928..24941)
                     /locus_tag="PC399_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2593317.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(24954..25499)
                     /locus_tag="PC399_002462"
     CDS             complement(24954..25499)
                     /locus_tag="PC399_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2593318.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT         8798 a         4272 c         3264 g         9358 t
ORIGIN      
        1 attatctaag tcttcatcaa acatttttat tatatttttt atctcatact tgtactcatc
       61 ttcattcatt ctataaagca ttaaatcttc tagtacatct ttcatatcga agtacacatc
      121 ttctaataaa taaaaatagc cttcctctat atctttcaat ttatctatag tatccatttt
      181 tttacttata tctttcataa tttctaaatt ttgtatattt acatctatga ttgttaataa
      241 gtacattctc acagttttta ttatttcaac agaaaaatct acaatattat aatttccttc
      301 ttttttatat aatgactcct taaatttgtt aaaacaatta tctaaattag aatttatatc
      361 gttaaatggt ggtcttatta tgtcacgtat tattaaaaga ttttgtatat ctacattatt
      421 tgtgattgcc tcatccaaaa taaataaatt atatgcatca tttaatgggt catgggcatc
      481 tccactaaac tctaagttaa caaattcaag taaatttttc aatgatatat agttcataca
      541 tctcatacca caatttatat atttttcttt tatatctaca aataaatctc taatgttatt
      601 tacaaacctt ggatggttat tttttttagc acttctcata tctgtgttat tgaaacatgt
      661 caaatccaaa ttaccaaaag tatatattcc tttaatatca gaaaatatgc caagccattt
      721 tttaaacttt ctcatgacct cttcataact tggtgcactc ttcaattctt cagaagatat
      781 atgtgttaat tcttgtatat gaggatacaa ctcctcatta cttactggtc ttattaaaga
      841 tttaaattta tcaatatttt ttgttttttc atcatacata actgctccga ttgcaattat
      901 atcagaatta aatactgctc tttttgattt agaatttggc atattcattt caaaatctat
      961 atatactctt ttcaatataa acaccctttc caattgttta tagtgattct ttttaactat
     1021 ttttattcaa aattttctaa gaaagaatgt ttctccccat ctttttgcca accaagtact
     1081 gaatcaagat ttacattttc tgccgcctta aatatagatt tttctacatt tttaaaattt
     1141 gattctaagc tctttattaa gtcttttatc tcatatcttt tattacctgt ttttttagca
     1201 gcaaccatac atgcacaatt taatggccat ataccactat tttgaataaa tcttataata
     1261 tgttcttctc ttatataata aagtggtctt ataattttta taccttcaaa attagtagaa
     1321 ttaagtttag gaagcattgt tttaaaattt ccagcacaca ataaattaag cattgttgtc
     1381 tctattacat cgtcataatg atgacctaat gcaagtttgt tacaccctaa ttcttctgcc
     1441 tttgaatata atgctcctct cctcattctt gcacacatat agcatggata atcttttgct
     1501 atttcatctg ctatctcaaa tatccttgaa tcaaataaat gtattggtat atttagatac
     1561 tcacaattat ctataagtaa ttgtcttata tttgcatgat atccagggtc catagcaata
     1621 aattcaacat caaaattaac ttgtccatgt ttttttaact cttgaaacat tttagccata
     1681 agaatactgt cttttccacc agaaatcgct acagctatct tatcaccttc ttcaaccaat
     1741 ttataatctc ttattgcctt cataaactta gaccataaat ttttcctata tttttttatt
     1801 atactttttt ctatatctct tactggaagt ctttcctcaa aaggaactat aatttcacag
     1861 ccttttcctg atagttcact catctaatca cctcgtatat ttatgttaat aatattttac
     1921 aaaaaagtct accttcatcc acaaaatact ataattttta ctttaatttt actaaataat
     1981 ttatagttta caataacaca taaacttata ataacataaa taacctcttt cttttattat
     2041 cttttttatt tttctcattt aatattttat tattagtaac gatttttaaa tgattgaata
     2101 ttatacacta gatagagtta cctatctatt tacaacaagg aggttttcat cttggaaaat
     2161 aagactagag atatcaaaaa aaattgtggt ccatctttgg ctagaccata cattaataat
     2221 caaattttca cagaaatgta ctgcctttct gatgcattaa aatttggaac tatatttcca
     2281 gaactaaact tactagagtc tgaaatgtac aacaaagaat tatatgctaa gccaaaaaaa
     2341 tatagaggag gtagaagata atgaaagact catctagagc tgaacttatg agaaaagttc
     2401 aggaaacatc ttttgcatgt gttgatatga acttatattt agacaatcac cctgatgata
     2461 aaaatgctat caacacttat aattcattat gtaatcaatt tgcgcaagct agatatgctt
     2521 acgaaaataa atacggtcct ttaactaact ttgggtatgc tccaagtaga tgcccttggc
     2581 aatgggctga ccaaccttgg ccttgggaca gagaatttaa ttattagaca tcaagtattg
     2641 gaggattgaa aattcatgtg gatatatcag aaaactatac aacacccagt taatataaaa
     2701 acttgtgacc ctagaatggc taaatttctt ataactcaat ttggtgggcc aaatggagaa
     2761 cttgctgcat ctttaagata tttaagccaa agatatacaa tgcctactgg taatatgcgt
     2821 gcacttttaa cagatattgg tacagaagaa ctagctcacg ttgagcttat atgtactatg
     2881 gtttatcagt taacttctga tgcaagccca gaagagttaa aagctgcagg tcttggttca
     2941 aactatgctc aaaatggata tggaatttat ccaacagatt caaatggtgt tccatttgat
     3001 gtaagaccta tagcagttat gtcaaatccc gtaaccgatt tacatgagga tatggcagct
     3061 gaacaaaaag cacttgcaac ttattatcaa cttataaacc taacagatga cgttgatgtt
     3121 atagatgtat taaaattctt gggtcaaaga gaaataattc attatcaaag atttggtgaa
     3181 gccttaatgg atgcttacga gttagaagaa tctcaaaaaa tgttctaaaa tttttatttg
     3241 tataactatt attatttgat aatttataat ttactgttgt gttaaaattt ataaaaaata
     3301 taaatataat ttattttcta taaacagaga aaataaataa aaagcaggta tcaatcaaat
     3361 agtaacatgt attatactat acattactat caatattgta cctgctttca tattataagc
     3421 aataaagaaa aatctattta ttaggtttta taataaaaac aattataaaa ctcttcttgc
     3481 atttacatat ctacttgaat agtagctact atttatatta gatattgtta ctttttttga
     3541 tgaacctgaa gatgcatgta tcatatctcc atttcctatg tatattccaa catgacttac
     3601 agaaccatta tttgaacctt gtgtatcaaa gaatactaaa tctccacttc taaggtctcc
     3661 tctatttaca tatgttccat acttactttg gtctctagat actcttggta tactaactcc
     3721 tacagatttt ttcataacgt actgcgtgaa tccagaacag tcaaaactat ttggaccttc
     3781 tgctccccat acatatggtt ttccaagtag tgtctttgca aaacttatta cactatcagc
     3841 tccttgactt ggagaattag tcgaataaga tggaattgaa gtatcaacag aacctttttc
     3901 agcaaggtat tgacttgaca catatccagt ttgattgcca taacttattt ttgtccatcc
     3961 tccactttca gaaatacttc caacctcagt accataactt aatgtagcta ttttagaatg
     4021 acttgtacct ggtcctgttc taacgttcaa tgactttgct gttacaacct ttgtaccctt
     4081 tacaaggtca tttgaactgt tttcagcatt ttcatttgta gagtcattta cactagttcc
     4141 taaatattta cttgaaacat aacctactct accattagaa cttatttttg accatccatt
     4201 tgactcagat atgtatccga cttcagtccc atatcctagt gttgatattt tagaactact
     4261 tgtacttggt cctgttctaa aatttaatcc ttttgctgtt acaacttttg tcgacttaac
     4321 tgaagtatca ctactttcat tgctacttcc aaggctattt attgctacat aatttccatg
     4381 cacataacct gtgtttccat tatatttaac ttttacccaa gaaccttctt ttcctacgta
     4441 ctcaacttta tcacctttgt aaagtttccc cattgattca tggtttgtac ctggtccttt
     4501 tctaaaattt accgcattcc ctgttattgt tctgctttct atactcacac tctctacttc
     4561 agactcatta gcatatgcta aattccccat agtaggcatt attatcgcag ttgccattat
     4621 actggctgct attctctttt gattaaaatt cataactttc cactcatctc ctcttttcta
     4681 ctatctttat ttcacttcaa aatataaaag aaataattga cactatcaaa atatcaatta
     4741 tttcttttat attaatatta ttacaaattt gtaactattt gtctatagta atatttgctt
     4801 accataaatt accatgtata aattttaatc tttaatagcc tgtaatttag taatatagcc
     4861 atcttttacc ttttttaaaa atggctatat tttttaactt ttttgtaact ttttaaaatc
     4921 cagttgagcc aaacccacct tttcctcttt cggagtcact taattctttt acttcttcta
     4981 tgttaatatc atagataggt tttactatca tttgagcaat tttcatatgc ttttcaacct
     5041 taaaatcatt tttaccatga tttattaata taattttaag ctctcctctg tatccttcgt
     5101 caatagttcc tggtgtattt aatacagtaa cagaatgctt taaggcaagc ccacttctag
     5161 gtcttacctg cgcttctgtc atagtaggaa gctctataca tatcccagtt ttgataagtt
     5221 tagtctctcc aggaggtatt acaacctctt ctatagaata caaatccata ccagcatcac
     5281 ctttgtgagc aaaattaggt attatagcat catcatttaa tttttttact tttaaattgt
     5341 acatcttata tctcctttca actttctata aatattataa cttaaatttc gacctcttga
     5401 agttttttat ttttttgtgg taatataatt attagtgttt taaaggagga ttttttatga
     5461 aacttgtact tgctgagaaa ccatctgtgg caaaaactat agcatcattt ttaggtgcaa
     5521 agaccagaca agatggttac tttgaaggaa atgattatat aattacttat gcagttggac
     5581 acttagtatc attatatgat atgaaggatt atgataaaga taaatactct ggctcttgga
     5641 aaatgaacaa tttcccattt gttcctgagg ataagtttaa atttaagatt gatagttcta
     5701 aaactaaaca attcaacact gtaaaaaagc ttttgaatag aaaagatgta gaatatgtaa
     5761 taaatgccac tgataatgac cgagaaggag aacttatagc atttttaata tttttactgg
     5821 caaaaaataa aaaaccagta aaaagaatct tggtaaatga atggacacca gaagatatta
     5881 caagaggtat aaaaaaccta aaagatgaag atgaaatgag aaatcttcaa gctgctggat
     5941 atactaggct tattactgac tggttgattg gaataaattt cacttctgtt gctacattga
     6001 aatacggaaa tgggaaacta ttaaacatag gtagggtaat tcttccaaca gttaaattag
     6061 tctatgatag agatatggaa atactaaact ttgttcctaa aacttattat gaaatagaag
     6121 ggcattttaa agctgaagct ggtgaatata aaggtaaata tgttaagggt aaagaaagta
     6181 aatttgatac acttgaagat gcaaataaaa ttatagcttc aattacatct gaaactggta
     6241 aaatactaga taaaaaagtt actatgtcaa aggaatatgc accaaaactt tttagtttga
     6301 catcacttca aggatatata acttctaagt attccaattt tacatctgat aaggttttaa
     6361 atgtctgtca atctctctat gagggtaatg gtaagggtgg atacataact taccctagaa
     6421 ctgattccat tttcttagaa gaaagtttgg cttcaaaagc ttctcaaact ttagataaat
     6481 taaaggttgg tcttgaatat gaaaataaaa ttaaattttc taaaactaaa agagtttttg
     6541 attcttctaa agtagactcc catagtgcta taattccaac ttatataatt ccaaaaagtt
     6601 tatctaagga tgaacaatta gtttatgatg ccataaaaga tagatttgta gcaaacttta
     6661 tgcctcctgc tgaatatgag aatacagaaa ttaagactga agtagataat tgtactttct
     6721 taacaaaagg taaggtgctt aaatcaaaag gataccttga ggtatataat aaggaagaaa
     6781 aaaacgattt acttccactt gtaaataaag atgatgttgt agatgtgtta gaaataaaac
     6841 cattaactaa gcaaactact ccacctaaac cgtatactga ggacacactt ttaaaggcta
     6901 tgaaaaattg tggtaaaaat gtgcctgaag aagatacaac agtgctatct ggatattcga
     6961 ttggaacatc agccacacgt gctgatgtgt taaagaagat aagtcaagtg ggctatgtaa
     7021 agaaaaaagg taaatcttat tctataacag accttgggaa aaatttagtt gaaatttttc
     7081 ctgttaaaga cttatttgat gtagattata ctggaaaact tgaaaaaagt ttaagcgata
     7141 ttcaaaaagg aaagtataca agaaaagaat atcttactaa tatcattagt tttatatatc
     7201 aaaatgtcaa tattataaaa caagatgctt caaaaaatat aaacacagaa gcatatactt
     7261 tcgattcaaa aactaaaaaa tttactaaaa caaaagaaaa aaaacagaaa aaagatgtaa
     7321 ctaatactga aaaagaagtt gctatatcaa aggattctaa taacaaaaaa gaagttaaaa
     7381 atggtgatga aaatcaatct cttggcaaat gccccatttg tcaaggtgat gtactagaat
     7441 ttgacaaagg ctttgcttgt aaaaatcata aagaatgtaa atttgttatt tggaaaaatg
     7501 ataagtattt aagtctttat aaaaagaaag ttaacaaaac aatggtcaaa aatattttaa
     7561 aaaaaggtga aactaaagta aaatctttaa ctgctaaaaa cggtaataaa tttgatgcta
     7621 cattaaaata tgataaaaat ccagatactg gctatttcaa ttggaaaata gagtttgaca
     7681 actaaatatt cataagaaaa gaaatagcag tagtataata atctatatta tattactgct
     7741 atttcttttc ttatgaagtt tcaacttttt taatagtgac ttttacaatc acactctaac
     7801 ctaatttctt tactatttct gataacttta atgtatgttc atctaataaa gttagttcct
     7861 cttcagaaag attagataat ttttttacta gagattcttt gagaatagct tttactgcat
     7921 caaataattt aaatgcttta tctgttaatt caaccctaag aatccttcta tcttttgggt
     7981 caggaaattt attgactaaa ccatgttcca ctaatctgtc aataatcgga gtcatatttg
     8041 attttaaaat tcctaaatta gaagctattt gagaaattga tgatgaattt gatgtcataa
     8101 gataaagaat taccttagca tgtgatggag ccattggaca ttcttcaaaa taagctttca
     8161 ttatatcaga gttattttga aactttttta acaaatcgct ttctttaaat atattatttt
     8221 caagcacaaa taaaaaatct aaaaaagagt ccgctatttt aagtatattg ttgttatcca
     8281 tatttgtaac atcccttcaa tagataatat aataaaacat tgttgtttaa tatagtataa
     8341 attataaact attaataaat ttaaaacaag aaaaagttat tgactcataa taattataaa
     8401 tttataatca aattagagaa ttataaattg ccaataaagg gaagtttttt aattagtcta
     8461 aaactaaact taaattataa gacactttaa attctacaac aagctagtgt gcaatatata
     8521 ataggaggag atatgaaaag atatttaata gttttcttaa tgtgtagttc actgttttta
     8581 gttggatgtg gaaaaacaga agaatctgaa cctgagaaac caatagcagt ttctgttcaa
     8641 aaagcaatag gtggagaaat tgaaaacaca aattctttct caggaactac aaaggttaaa
     8701 gatgaaacag ctgttactgc acagactgtt ggcacagttc aagaagtgta tgtaaaactt
     8761 gggcaaaatg taagaaaagg agatgaatta ttgagtataa gcagtccaga gctagaaaat
     8821 agtgtaaagc aaagtaaagc ctcactagat ttagcaaaag caagttattc aagtgccact
     8881 ggtggttcat tagaggcaca agtaaatcag gcaaaaacag ctcttgacaa tgcaaagatt
     8941 caatatgagg agtctcaaag aaattatgac aataataaga ttctttatga acaggaagtc
     9001 attagtttag accaatttaa aaaaatagaa ttttctttag agcaaacaaa acaacagtta
     9061 gattctgctc aaagagcata cgatacagca acttcaaaaa gtatacctca agctaaagca
     9121 cttgctaaaa aacagcttga ccaagctcaa gtatcatata atttagcaat gagtaattta
     9181 gacaaattaa cattaacatc tccagtagat ggtacaataa cagctaaaaa ctttgattca
     9241 aaggaaatga taacacaatc acaaccagct tttattattt ctaatcctaa tatactagaa
     9301 gttgatttaa atgtagcaga atcagatata ggtaaattta agaaagatgg aaatgtagat
     9361 gttataattg aagaccaaag gattttagga aaaatagatt atgtaccttc agtagttgac
     9421 cctcagacat ctttatatcc tgttaaagta cttgtaaata atacaaacaa caaatttaag
     9481 gcaggtatgt ctgctcaagt aaacttaagt gttgaaaaag aaaatggtgc tgttacagta
     9541 cctaaaaaag caatatttga agaaaatggt aaaaaatatg tatacatagc aacaaaagac
     9601 aatatagcaa agaaacactt ggttcaaact ggaattgtaa cagaggataa aatagaaatt
     9661 aaaagtggtg taagtgataa agatacagtg attatcggag ggataagtct tatttctgac
     9721 ggaactaaga tatttcctgt agaaaaggag aagtagacta tgaatttaac acagacttcc
     9781 gttaaacgtc ctctcacaat cataatggtc tttttagtat taatagtttt tggtggtatt
     9841 ggatataaaa aaatgtcaat aaatctaatg cctgatatag aaataccagt tgtaatggtc
     9901 atgactactt ggacaggtgc tggtcctcaa gacgttgatg aacaagttag tcaaaaagtt
     9961 gatgaaagtt tatctgccgt ttcaaatgta aaatctacta tatcaagttc acaagaaagt
    10021 gtttctatgg tagttgctca gtttgaattt ggaactaatt tggatgaaat aatgaatgat
    10081 gttaggtcta aagttgatgc attacagacc agcctcccag atgatgcagc taaaccaaca
    10141 gttttaaagc tggatatgaa tgcacaggct ataggtcaac ttgtcatatc tggaggaaat
    10201 gaaaatagtt cacaagcctt aagaaaatac gctgaagatg taattcagcc taaaatagaa
    10261 tccatagatg gtgttacttc agcagacctt aaaggtggag aaaaagcaca ggttaatgta
    10321 atagctgacc cagctgttct ttcaaattat ggagttagtt tatccactat aaaaggtgtc
    10381 ttatcctcta gtaataaaac attcccttat ggttcaatta ctcaaggtga ggataaaata
    10441 gttttaagag cgattgataa attagaatcc ctagatgata taaaacagat tcaaattcct
    10501 gtaaaaggag gaaatacagt tagattagat aatctttgta gtgtagatta tggatatgca
    10561 gacaaagata gtatttatag atacaatgga aaagacagtt tggtaataga tatttctaaa
    10621 caacaagatg ccaacactgt aaaagttatg cagagtgcat acaaatacgt agatgaattg
    10681 aataaggaaa atcctcaatt taaaattaaa attgctaatg atacaagttc atacataagt
    10741 gaatcaataa acagtgtaat gagcaacttg cttataagtg ctgttatcgc atttattgta
    10801 atatttgctt tcctaaaaag tgtaagagct tcactagttg ttgcagttgc aataccaatt
    10861 tctataatag gtgctatagc aattctctac tttactggtg aaagtttaaa tcttattact
    10921 gcaagttcat tggtaatttc agttggtatg gttgttgata atgctacagt tgtaattgaa
    10981 aatatattta aatataggaa aagtggtaga ttgagtcttg atgattgtgc tattgaggga
    11041 acaagaactg taacaaatgc aatacttgca tcaacattga ccacaatagc aatattctta
    11101 cctatcatgt ttacagaagg tatagctaaa ataacttttg gggcattagg taaaacacta
    11161 atagcttcac tagcattctc ctttataggt gcaattacac tcgttcctag tgtatttgca
    11221 aaattaagca gaggaaaaaa tagtcaaaag atgcaagaaa aagatagtcc tatatttgat
    11281 aaggtaagtg aagcatataa aaaattatta agtgtatgtt tgagacatca acgcttagtt
    11341 gtaatatcaa gtttacttat ttttgtagct tccttatttg gaatgggatt tataggtatg
    11401 gactttatgc ctgcagcaga caagggtgaa ttatctattt ctatagaact tccaaaaggt
    11461 cttagcctag aatccaatga ttactatgta tctatgactg agaaaaaagt ctcagatatt
    11521 ccagaaatta aaactttaat aacaactttg acatcagatt caaataatag taaagcaagt
    11581 atagcaattg aattagaatc tcaaaaaaat agaaaaagaa ctactgatga aatagaaaaa
    11641 gagataattg atagagtggc tactgtacca gattgtaaaa taaatgtaag tcaaaacagt
    11701 agtgttatgg gtggagctgg aggagcagac ttcacattag aaatgaaagg tcctgattta
    11761 gatgtattaa aaatactggg aaaagaagct gaaaaagaat ttagctctat aaaaggattt
    11821 agaaatgttg aaacttctct tacagataca acacaagaag ctcaatttac tatagataaa
    11881 cataaggctc aagaatatgg tgtaaatacc gctgaaattg caggcatgtt acgtacagca
    11941 ataaatggtg actccgtaac cactgctact attgatgatt acaaagtgga tgtaaaccta
    12001 aaatttaaag cagatactat tgatagctta gaagatataa agcaactaaa agtaaactct
    12061 gcaactggaa aagaagttcc tcttggtgcc ttttcagata taaaaatggc agatggtcta
    12121 caatctatat ctaggactga tggagatttt tctgtaaata taactgctac acttgacaat
    12181 ttagatgctg gtactgctac aaaactagca ctagaaaaaa cgaataaaat ggtaattcct
    12241 aaagattact ctataggtgt tggtggtgaa acagaaatga tgaatgaatc tatggcaagt
    12301 cttggatttt caatggtcat agctatagca ttagtttata tggtaatggt agctcagttt
    12361 gaatcattta gtaaaccatt tataatcatg ttctgtgttc catttgcatt cgttggagtt
    12421 gtgctttcgc ttctagttag tggtcaaagt ttaagtactg tcggtatgct aggtataatc
    12481 ttacttatcg gtattgttgt taataatggt atagttctta ttgactacat agagcaactt
    12541 agaaaaacaa atcgtaacaa tgatttaatt ggtttggttt caattggttg tgcagcaaga
    12601 ttaagaccag tcttaatgac tacagcaaca acaattttag gtatgttgcc aacagcacta
    12661 tctcttggtg aaggtggaga aactatgcaa ccactagcca tagttataat gggtggtcta
    12721 actgtatcta cacttataac tttggtgctt atacctacta tatacttaat atttgataaa
    12781 tgggaaaata agttttcaga caaaatgcct aaaatatttg gtaagataac agataaaata
    12841 ggaagtataa aacgtaaaat aataaagaaa ccaaaattga aagctaaagt ttaaatagaa
    12901 tggagggtga ttgtattgaa taaaaaaatg agcaaaatag ttgcaattag ctgtggtgtt
    12961 ggtataataa ctgccaattt aatcccagtt tacgcagata ataatacttc ttctataaac
    13021 aaagttgata attctatttt aagtgactca aataaaagaa catctgaaaa ggaaattttg
    13081 actgctagag aagctgttag tgctgcactt aaaaatagtg aaaaattaaa aatgaaatca
    13141 gaagaaataa aaatgttaaa agaaaagtta gaagtacaag atgagtttga tagttttact
    13201 ggaagtgaca actcgtttcc atacgaccaa attggacttc ttaaaaatca aagtgaacag
    13261 gctaaaggct ttatggagga ccaaatagca aatgatatta ctaataaatt taacgattta
    13321 gtttcaagag aaaatgagct agataaaata aaaaacaatc ttgaaataaa aactaaagaa
    13381 attaaagata tgaaattaaa aaaagattta gggttagtaa catctttaga aactgaatca
    13441 gctgaacttg aacttcaaac actacaaaat actcaaaaag ctaaacttca agaactaaaa
    13501 aacaatcaag actattttaa gttattaact aatatagatt taaataatta tcaattggat
    13561 aaagaatata gatttgaatc ttttagagta ggtggctcag ttgattcata tatggaaggt
    13621 aaggttaatg aatatttaaa atacgaccaa ttgattttag aacgtacaga agaaagcttt
    13681 aatgataaag atgagaacaa agcagattta ccagatagac ctaaccttac aagaccagtt
    13741 gctcctacaa aaccagagca aggtaaccta actgatgatg aatacaaggt tcttatggat
    13801 aagtatgaaa aagattatga tagatataaa aaagaagttg aagcatacaa tcttgaaaga
    13861 cagacttatg ctgctggcct tactacttat gcaaattatc ttcaacaaaa atttaatact
    13921 gaaaatggtc tagtaacatt agaagacagt aaaaaagctc ttaaaaaagg gttaattgat
    13981 agctatgctc aattacttgc tttagaagat acaattcaga ttactaaaaa acaacttgat
    14041 ttatcagaaa aacaattaaa aaatactaaa ttaagatatg atttaggatt aattactcta
    14101 actgattata aaaaacaagt ggtaagtaat gaagatgcta aaaatagcta tgacactctt
    14161 attgtgaatt ataactcact aaaaaatggt atagaaaaac cttggatact taatactgga
    14221 aaataattct ggtaaataga aagattactt taacatacgt ttgattaaaa aatagttatg
    14281 catactcgtg gctttagtca tggaagattc agaaggcaaa taatagaaat agattagtaa
    14341 aaatatattt acttataaaa ctgtatactt ttatagaggc tatctcaaaa tagagataag
    14401 cctctttttt attgtttaaa ttataaaaat atatttgatt ccatacaaaa agagtgcctt
    14461 ctgaactaaa aagttcattt tgagacaccc tcaaaagctt aactctaaat ttaattattt
    14521 tttatcaaat gtttcatcta ttttacatct caccatgtta acaaattcag aaatattata
    14581 tcctctaagt ttgcatccac atttaggaca ataatccagt ttcctagata ccaaagccct
    14641 acaactaggt tctggacatc ttatttttag actctcatct gctactttaa atccacatcg
    14701 tgggcatata ctacctggac gtgatgatac ttcctttcca catttaggac actttgtcaa
    14761 ctttatcccc tccagtaaac tcaatttaat tgatagttga aatttctctc tacaataata
    14821 tatgcaatat aattaaatac gtttcactta caactatata agttaaattt ttataataaa
    14881 aatcccctat attcaattta caaatactta atttgataca ttgaacaata ggggatttta
    14941 tttttattta gttacatatt ttgcagtcta ttattatcta ggatttttta tagatgcttg
    15001 agctgctgca agtctagcaa ttggcactct aaatggtgaa caagatacat agttaagtcc
    15061 tacactataa cagaactcta ctgaagatgg ttctcctcca tgctctccac atattcctag
    15121 ttttaattct ggtttaactt ctctacctaa tttagctccc atcttaacta acttaccaac
    15181 accattttgg tctagtactt ggaatgggtc tttttctaat atttcttcat ctacatattg
    15241 acctaagaat ttaccagcat catctcttga atatccaaat gccatttgag ttaagtcatt
    15301 agtaccaaaa ctgaagaagt ctgcttcttg tgctatttca tcagcagtta aacaagctct
    15361 aggtatttct atcatagtac ctacagtata aggaacttct ataccttctt cttttattat
    15421 agcgtcaaca gtttctacta tcatttttct tattattttt aactcattta cttctccaac
    15481 taatggaacc attatctctg gagaaacttt aacacctgct ttgattgctt ctatagcacc
    15541 ttgtattata gctttagatt gcattacaca tatttcagga taagttattg caagtctaca
    15601 acctctgtgt ccaagcattg ggttaaactc atctaaatca actattctct tcttaatatc
    15661 acttactttt attcccatag attttgaaag ttcttctata gtctcatcat catgaggcaa
    15721 aaattcatgt agtggtgggt ctagtaatct tatattaact ggttttccat ccataacttt
    15781 aaataactct acaaaatcct ctctttgcat tggtaatatt ttttcaagag ctttttctct
    15841 ttgttctact gtattagata atatcatttc tctaactgct ggcaatctag cttcatcaaa
    15901 gaacatatgc tcagttctac atagacctat accttctgca ccaaactcta tagctgcacg
    15961 agcatctcta ggattatctg cattagttct aaccatcata catttgtgtt tgtctaccca
    16021 gttcattaat ttttcaaaat ttccagttaa agatacttct gttttcttaa catctcctag
    16081 gtacacattt cctgtagaac catctataga aatgtattct ccttctttta taactacatc
    16141 atcaagtgct cttatttctt tattaaactc atcaactttt atttctccac atccagcaac
    16201 acaacattta cccatacctc tagcaacaac tgctgcatgt gacgtcattc cacctctagc
    16261 tgtaaggatt ccttctgctt taaccattcc ttctatatct tctggagatg tctctagtct
    16321 aactaaaact actttttctc ctttttcatt tgcttttact acatcatcag cattgaaata
    16381 aactttacca cttgctgcac ctggtgatgc tggtaaacct tttgctataa ccttggcttc
    16441 ttttaatgcc ttatcttcaa attttggatg taataattgg tctaattgat taggctcaat
    16501 tctcatgata gcctcttttt catcaattat tccagcttca actaaatcaa ccgctacatt
    16561 tattgctgct gctgctgttc tcttaccatt tctagtttgt agtatatata atctttcatt
    16621 ttctatagta aactctatat cttgcatatc tttataatga ccttcaagta tatgagtaat
    16681 ttttacaaat tcatcgaata cagctggcat tacatctgca agagttgata tattttgagg
    16741 agttcttata cctgcaacaa cgtcttcacc ttgtgcattc attaaaaatt caccaaataa
    16801 tttattttct cctgtagctg gatttctagt aaatgcaaca cctgtaccac tagtttctcc
    16861 catattacca aataccattg attgaatatt tactgctgtt cctaagttat gagctatatc
    16921 attcaattta cggtatacga ttgcacgagg attattccat gatctaaata cagcctctat
    16981 tgcaagcatt aattgctctt ttgggtcttg tgggaaatca ttttttattt cttttttata
    17041 aattgcttta aattctttta ctatttcttt taaatcatca gttgtaagtt ctgtatcaac
    17101 tgtgtatccc tttgcttctt ttactctgtc taatacattt tcaaatttat attttggaac
    17161 ttccatagca acatcagaaa acatttgtat gaatcttctg taactatcat atgcaaatct
    17221 ttcattttgt gtagcctctg ctaagccaac aacactattg tcattaagac ctaggttaag
    17281 aattgtatcc atcattcctg gcatagagaa tactgcacca gaacgaactg atactaataa
    17341 tggattctta ttgcatccta atgttttatt taagtctttt tctaaagtag ctaaatgctc
    17401 ttctatttct tttattatct ctgctcttat actttcattg ttaacataat aatcattaca
    17461 tgcttctgtt gttatagtga atccaggagg gacaggtaag cctatcttag tcatttctgc
    17521 taaattggca cctttacctc ctagtaaaga tttcatgtct ttgcttcctt caccaaaact
    17581 ataaacatac ttagtttcca ttgttaaatc ccccattaat ctttttattt taatttatat
    17641 catttttatt aatagacatt attaaatttc tatttctttg tatatcttta gaccattttc
    17701 tttcattatg tctaaaatta tacctgctgt ttcttctatt gccttatttg aaacgtttat
    17761 tactgggcat ccaattcttt tcattatctc ttctgaataa tctaactcct gaagtattct
    17821 ttctaaatta gcgtagtttg ctttactgga caatcctaaa gcttttaatc tttgggttct
    17881 tatctcattc agcttttcag gtgaattagt aagacctatt atttttttag tatctatctc
    17941 aaacacttct ttaggaattg gtatttctgg aactaatgga acattagcta cttttatatt
    18001 tttattagct aaatacatac tcaaaggagt tttagatgtt cttgatattc ccactaaaat
    18061 tatgtctgct tgaagaacac ctctagggtc ttttccatca tcatatttca ctgcaaattc
    18121 tatagcttca actctcttaa agtaagattc atctaatttt ctaattatac cagcttctct
    18181 tttaggtttt cttcctgttc tcttgcttat ttctcttagt atatctgtca ttaaatctat
    18241 acaactaaga ccttctcttt cgcaatatct ttcaacaata ctgcataatt cttcatcaac
    18301 taatgtatat acaattaaag cgttttcagc ttttgcattc tccaaagttt caagtagtac
    18361 tcctttatta atcatgtatg gaaatctttt tatttcataa gtttcattca cataaaactg
    18421 agacatacat gcctttgcaa cttgttgtgc agtctcccct actgaatctg atactgcata
    18481 tataattagg tttttcacca gcatacctcc ttaattgtca actatttcta agaatagctt
    18541 agttatgttg gtttttgata ttctcccaac aactctcata tgatttttgt cttctccctt
    18601 atattcaaca atcggaatcg aatctacttc atgttctatt atttttcttg aagctaataa
    18661 aacacttgct cctttagtta aagtgactac atttggagtt cttgtcatta tcatacctat
    18721 tggcatttta tttatatcag aacctcctat agttgctttt agcaagtctt ttctggaaac
    18781 aactccacat aactcttcat tctcatctat aataactata ctccctacat cagataaaaa
    18841 catagtgact attacatcat atatactttc atccttctta gctagtacag gcatgctcat
    18901 tatatcctct actgtttttt cttttacact tttaccaatt aaatttattt cactaactcc
    18961 tgaataaaaa tatccaactt ttggtcttgc gtctaatatt cctgtcattg ttaatatagc
    19021 taaatcagat ctaagtgtag cacgagtcac attcaagctg gaggcaatac tttcactcgt
    19081 tattggttca ttttccttaa ctatatctat aatttttagt tgtctttcat taagctgaat
    19141 aataatcacc ttctttcatt gtgctatact ttttatcttt tcgcatctta tatagtatat
    19201 tatataacta tatataccat tctgtaaaca gattttttat tgaatacgat atatttttta
    19261 ctaagttctc tcgcttaaag atatttatta tatattaatg ggggatattt ctttaattaa
    19321 tagaactata ttagtattac agttaatatt ataatacata attggtcatt ttaattcatt
    19381 ttatattaaa atttttaata atattatttc aatgtgaata taaaattgca gaactaattc
    19441 aaaataattt ttacgactac aaataaccta taatatactt ttaaagaaag tatcctaaca
    19501 agaatatata aaaatttaaa agggttgatt taaaaataac catcaaccct ttttactaat
    19561 atctatttat attaccatca aatcttaccc aaatattttt tcttgcaatt gtcttaaatg
    19621 atataaagta gtatcttttt ctatctctac catatcgtaa gttataactt cacctttttt
    19681 aatatctttt acaactttag tctttttact tatcaaacca ataggtacag cattcatagc
    19741 ctttgctaca tcgtattttt ctatagtacc atagcatgta tatgctccat agccatccat
    19801 aaactcacct tttttcaaat ctttcttagc tatagtcata acttctgcta ctagtccagc
    19861 tttaggtact attgtagcct tatggtctaa gactgccata gctgctgata aaggagtctc
    19921 taaactacat aaatgatatg gtctatataa tatgtaatta ggtccatcac ccatacttaa
    19981 atattttagt tcatcattaa ctgcttttaa tttatgggct actactacga aaactccagg
    20041 tgctatacca tttacaaaat caactacacc ataattatct aatactccac cttcgctttt
    20101 taatgcatat ttcttaggta cttcattaac tgtagcttca ataccatgac cacctcttac
    20161 atcaggcaca aatccagttg cattacacat taaagccatt tctaccattg ttttagttcc
    20221 ttctttaaaa gaagctatca tatgaggtga agctcctttt ctttctgctt cttctctaac
    20281 tgtatcagga ttacaatcta aatctaattt attattttta cctttaccta tcactctaac
    20341 ttcaaacccc attgcatctg caaaatcaaa taattccatt actgcacctg gctcatctcc
    20401 tgcagaacct gtaaatatta ctccagcatc atctgctaat tttttaagta ttggtccaac
    20461 aacacaatca gtttcagcat ttaacataac tatatgtttt ttatttaaaa tagagtttaa
    20521 tgctatctct gcaccaattg caatacctcc agttgcatct ataactacat ctattgactt
    20581 agttttacaa gccacaagaa aatcattaca tgtcaatact tttccttctt gtaattttat
    20641 ttctgcatcc ttttcatttg ttacattatc tataatttct tcatctattc ctgcatcagt
    20701 aaaagcttta tgagccaact ccaaatttat atctacaaca atagaagctt caataccttt
    20761 catatttgcc atttggctta ccattccatt tcccatatgc ccagctccta taagagctac
    20821 ttttattttt ttaccttcct cttccaattt tgcaagtttt ctgtttaaat ctaacatacc
    20881 aattcctcct gttataataa ttttattttc aaattaagaa atagttatta aatctccaat
    20941 agttatatct ggatttccta tttccacatg tatttctcct ggtaaattta cttcacattt
    21001 tccatcaaat ttgattgtac aatgcccaat ttctctaaga gtcttctgag caatatcacc
    21061 aactgaaact atacaataat ctctattccc tatagtaagt gtatctccta tttcaacatc
    21121 actttttaac gttgctattg aatgaattac agatatatcc tttaagtcgt catcattaat
    21181 ggtttcatca tatataataa gacaattgct ttcatccatt aactctttaa ctaaactgcc
    21241 tatcccactt atcttaactt catatttcat gacattcctc ctgataattt atatttatat
    21301 tgagttaatt aaaagaaaca tttatttttt cttttataaa ttttatagct tgtacaatac
    21361 tctcttcatc tttaaatttt tgctctgcac tatatatatc taatccttct atttcttcaa
    21421 tctctttaaa tttagccatt actgtacgtc cttttaaaat ttctccaaac tttataaacc
    21481 catctgaatc tgaagatata atggctatac ttcctttaga aaaatagctt ttctttttgc
    21541 ctgtagacac tatatcaccc ttttttttag ctctatttaa agttttaaaa tacaatttat
    21601 tctgcttcat tataaatata gtatttaaaa tataagcaac aaataaaatc actaataatt
    21661 tcatatttat acaccttcca aaaatcacat tatttttaaa tctgtataac aactataaat
    21721 gctgtacaat agttatataa ggtttttaag taacaccttt ttaaattcta aaaaagttct
    21781 acatttactt atcttattcg ttaattttac atcttggcct atctttaaaa cttcttcata
    21841 aatttttact aataagtctg agtctatctt attctcacat ggaaacattg tcataagaat
    21901 tatttttata cttctatttg catattctat tggttcttct aaaattccaa ctgccattaa
    21961 aaacttatct gatttttcat ttacagaatg aggaaataat aaacctttat caaatactgt
    22021 gggagatttt tcttctctat tgactatatt ttttctaaac ttatcatcaa tgcatcctaa
    22081 atccataaga tggtccatca ttttttctaa tgaagcaagt atagtctttt catttaatat
    22141 cataaattta tcttcatcta acaaattatt tattagtaaa tttgtagaat tagtagtgtc
    22201 aatattccct tctcttaggc acaatactgt ttttaattgt tctcttagtt tgttttcatc
    22261 aaatattgta tttattttta atactattgt atttatattt gatgtgtcta aatcaatagt
    22321 tgtaaaaact atatcatatt catctaagtt aatatttctc aaattaaaac ttgaaagagt
    22381 atcaatttta ggattatttc ctatcagttt ttctactctt attttcaata gttttgctgt
    22441 tcctagacct gtactacata taattgctat tttttttatt gaatagaatc tactgctatt
    22501 tctttctaaa tatccactga aatggattgc tatatatcca atctcatctt ctgaaacttc
    22561 taagttatac tttttctcta ttacttttcc tgctatttgt gctaatttaa atggaagtat
    22621 ataattttgc ttaatctctt ctaacaaaac attttttaac tttatattaa ataatagcct
    22681 gtttaaagta aaactaagat gatatcctag acctactctt aattcatcct catttataaa
    22741 aatacccata tcgacatata tttgattaaa tatattttcc atcaactcat caatgttttt
    22801 actaattcta ctactattta aagcacattc tgatgctggt gcatttccac taactaacgg
    22861 tatagttaaa taaataattt cttcatcatt taaagtacat tcaaacattc ctgatattat
    22921 atgttttatt tccctagcaa taaagtattc atcatatgat ttaagtaaat caaactttac
    22981 atcataatct tttactttct tttcttcttt tactctatag actaaaacta ctatgtagtt
    23041 aataacttct cttaacattt catctgtaac ataaaaatta ttttttttaa ataaagtcaa
    23101 tagtttttct tctaaattta tgatttcttc ttttttgatt cctttaaaat actttgaatt
    23161 actgaaatct ttccttgagt ttttatacaa ctgatttaat ataaacagtc taatatcaag
    23221 ttcatttcca tttaatttca tacctgtatt ctgtttttta atcaagttta aattatatga
    23281 agccaaaact ttttctacat actggaaatc atttactaaa gtagttcttc ctacacacat
    23341 ctcatcagct aaactatcaa gtgtaataaa tccatctagt tctatcaagt aatttagtat
    23401 aaactctact ctatcttctt ttgaatttaa tgaaaataca ttttttctat tcatatcaat
    23461 aatcttattg agtatctgct catcttttat ttctagttta tagccttgac cctttatata
    23521 tttgattact gctatatcat tacctaaatc tttattcaat tgttttatat acgtccttac
    23581 acttctagtt gtgacattca attcatttgc tattgagtta atactcacaa actctttttt
    23641 actacacata atcaatatct taaatatctt gcggtcaata gaaggaattt tctcagttac
    23701 actcattttg tatctcctcc ttgatttcat tctagtacaa atttgaaacc aaattaatac
    23761 ataaaatttc actttaataa aggaaaattt agtattaatc taactcgata attacagcta
    23821 attaatagta taaaagctag tctttcatta acaaaaaatc ttgactagct taaatttata
    23881 tttacaattt tattaattaa ttccaagcct tatattgaca ttaaatatta tctcaatgca
    23941 aatactgcaa ctccatatgc aataataaca gctattggac cagttattaa acgagagtaa
    24001 aataaagctg gaacaccata ctctacagtt tctggttctg cttctcccaa acttaatcct
    24061 actggtacaa aatcacatcc agcttgccca tttattgcaa ataaagctgg aagtgctaaa
    24121 tatgctggta ttgcaccaag accaatttgt gtacctacaa gagtccctac tacttgagct
    24181 ataactgcac caggtcctag tattggtgat aagaatggaa gtgtgcaaat tactgatata
    24241 atcaataatc caccaatatt acctgcaagt ggagatataa ctcttgcaat ccagtctccc
    24301 agtccagatg ctaaaataat tcccataagc atacttacaa atgccataaa tggtattacg
    24361 ttattcatta ccatttggat agtatctcga cctgcatcat aaaattttga tactacttgc
    24421 cctgcccctt gaccaataga aagtattatg ttctttttag catattcacc ttgtgctttt
    24481 gacttaacct catcataatt atcaggcttt ctaatagaac tcttattttc gcttttattt
    24541 tcttgagatt tttgtggcat atcttcccca tctacaacac ttatacagtt aggattaaca
    24601 tctgatacat aatattcttc tgttataaat tttgctaaag gacctgtttt tccaactgga
    24661 tttacattta ttgtaggtat ttttttcttt ggataaactc cacatctagc agttccacca
    24721 caatcaataa taactactgc tacttcttca tcagaaactc cagttttaaa tccatctaca
    24781 atttcacaac ccagtagttc ttttattttt aatgttactt catggatacc accagcagtc
    24841 attgacagta tcttatttct attcccttct ttttttatat acaaaggacc tccccagcct
    24901 gaagttccct tttctatttt taatatttta ttctcctcca ttttatctac ctcctaagcc
    24961 ttatttgttg ctctattttt tcttgttaga aaagcataca ttttttctgt tactatacct
    25021 cttataaaaa ttacaattag accaactata aaataccaca atgccaatgt agtagtttct
    25081 ttacctaaag tagtcaaacc tgcagatatt cccaaccata caaaaagttc tcctgcattt
    25141 gcatgtggaa aaagacctgt aattggatgt acaaatgaaa ctgctgcatc ataaaatcct
    25201 gctttgtgct cttcttttaa aaatctacca aatgtatagc acataggatt tcccaagaaa
    25261 aatactgata ctaatggaag tagaatatat cttgtaaaag agttctttcc tagcttagaa
    25321 gcccaattat taactctttc ctcaccaatt atcttaatca aagagttaat agctaccatt
    25381 aatacaatta gcgttggaag agtacctgaa acaagaccta taaattgttc tccccctttc
    25441 tgaaacaaac cgataaacca ctctgcacct ttacttagac ctgtaataac gttttccata
    25501 aaattataac ctccctataa tatctttaaa tttttaattt tactgcttac cataaatttt
    25561 acaaattaca tcttttttct gtactatatg ctaatattgt aaatgttttt ttattaatat
    25621 cattttaata taatattttt gataaataaa tacatctttt ttcaatttta taaaggaaaa
    25681 aattgtacta at
//
LOCUS       DAMJWS010000059        25586 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMJWS010000059 DAMJWS010000000
VERSION     DAMJWS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.059"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(236..616)
                     /locus_tag="PC399_002463"
     CDS             complement(236..616)
                     /locus_tag="PC399_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2593319.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(817..2010)
                     /locus_tag="PC399_002464"
     CDS             complement(817..2010)
                     /locus_tag="PC399_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2593320.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGIYLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            complement(2073..3329)
                     /locus_tag="PC399_002465"
     CDS             complement(2073..3329)
                     /locus_tag="PC399_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2593321.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            complement(4125..4859)
                     /locus_tag="PC399_002466"
     CDS             complement(4125..4859)
                     /locus_tag="PC399_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2593322.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(5865..6503)
                     /locus_tag="PC399_002467"
     CDS             complement(5865..6503)
                     /locus_tag="PC399_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2593323.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(6506..7363)
                     /locus_tag="PC399_002468"
     CDS             complement(6506..7363)
                     /locus_tag="PC399_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593324.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(7365..7739)
                     /locus_tag="PC399_002469"
     CDS             complement(7365..7739)
                     /locus_tag="PC399_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2593325.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            complement(8888..10078)
                     /locus_tag="PC399_002470"
     CDS             complement(8888..10078)
                     /locus_tag="PC399_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2593326.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(10094..12049)
                     /locus_tag="PC399_002471"
     CDS             complement(10094..12049)
                     /locus_tag="PC399_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2593327.1"
                     /translation="MEKIKIENSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(12066..14132)
                     /locus_tag="PC399_002472"
     CDS             complement(12066..14132)
                     /locus_tag="PC399_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2593328.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            complement(14459..15109)
                     /locus_tag="PC399_002473"
     CDS             complement(14459..15109)
                     /locus_tag="PC399_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2593329.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(16213..16548)
                     /locus_tag="PC399_002474"
                     /pseudo
     CDS             complement(16213..16548)
                     /locus_tag="PC399_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(16646..17326)
                     /locus_tag="PC399_002475"
     CDS             complement(16646..17326)
                     /locus_tag="PC399_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593330.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(17323..18249)
                     /locus_tag="PC399_002476"
     CDS             complement(17323..18249)
                     /locus_tag="PC399_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593331.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(18322..19239)
                     /locus_tag="PC399_002477"
     CDS             complement(18322..19239)
                     /locus_tag="PC399_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2593332.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRAIVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(19239..19928)
                     /locus_tag="PC399_002478"
     CDS             complement(19239..19928)
                     /locus_tag="PC399_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593333.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(20336..20631)
                     /locus_tag="PC399_002479"
                     /pseudo
     CDS             complement(20336..20631)
                     /locus_tag="PC399_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21705..21923)
                     /locus_tag="PC399_002480"
     CDS             complement(21705..21923)
                     /locus_tag="PC399_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2593334.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(21924..22331)
                     /locus_tag="PC399_002481"
     CDS             complement(21924..22331)
                     /locus_tag="PC399_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593335.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(22417..22905)
                     /locus_tag="PC399_002482"
     CDS             complement(22417..22905)
                     /locus_tag="PC399_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF2593336.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(23110..24231)
                     /locus_tag="PC399_002483"
     CDS             complement(23110..24231)
                     /locus_tag="PC399_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2593337.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTQKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(24604..24774)
                     /locus_tag="PC399_002484"
     CDS             complement(24604..24774)
                     /locus_tag="PC399_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593338.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(24946..25389)
                     /locus_tag="PC399_002485"
     CDS             complement(24946..25389)
                     /locus_tag="PC399_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2593339.1"
                     /translation="MIEIKTIEINKKSQIDLLILADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         8828 a         4339 c         2690 g         9729 t
ORIGIN      
        1 aactttatat aaagaataga aacaagttct agattactat agagatgaca tatcaaaata
       61 tttttaaaaa gcaatttaag tattagaagt aattgaagtt agtaaatata aatgcgttta
      121 cttttaagta ttgcttaagg gaataaaaaa taaaaataaa ctctgcaacc atctaaaaat
      181 agttgcagag cttacaaaac caaaattatc taaattgtaa taaaaataat aacatttata
      241 cataagcttc ttcccaattt actacccagt tttctaacga ctttatcttt tcctctgtta
      301 gctcctcttc gtgaaatgat actaataaat tccttataaa attattcttc aaaagatttt
      361 gcacatttct tgatataaac tctaaatatg ccttttcttt gattgcaact gtataataca
      421 tacacttgcc tgtttcttga acatataaga atcttttctt tgatagtttt gataaagttt
      481 ttaaagctac tttttctggt aaattatgtt cttctttcat ataatcaata atctcatatg
      541 aattggtttt tatatctaaa ttccaaataa atttcataat aactaactct aattttgata
      601 atttttttaa acacatagtt atcactccgc ttctttatct atataattaa atattacaat
      661 aataatttca caaaatcaat tctaataatc ataacatacc ttaaaatctg ttcttaactt
      721 ctaccttctt aatctcccaa atgatttttt tatattgtat tcaatagtat aatccttttc
      781 caaaatataa cttaccagtt aaaataatct ttatttttac ttattctcgc cttttacaga
      841 agttaataat tttttataag ttacatcaat tccaaaagct ccaagaggtg cagttataag
      901 tatagctaga actgctaacg tcaaaataag ttgtccagat gccatcccca ttgcaagtgg
      961 tacacctcct attgccgcct gtactgtagc ctttggaata tagccaatca tacagaacaa
     1021 tctttccttt attgtcaaat ttgtcttaat aaggcagaag aatactccta ccatacggaa
     1081 taaaagaact ccaaatatta aaattacagc tccaattcct gctttaaatg cataacttat
     1141 atctactgtt gcccctacca aaacaaacaa gagaatttca gcaccaaccc atagtttcga
     1201 atactttata gacaatcttc ttgcaactac acgatatttt atctgaagga aaatcccaat
     1261 actcataact gctaataatc cagaaaatgg cacaataccc tctaaagcag tctctaaact
     1321 tatcattaaa aatgagatac ttaaaagcaa caccccttta gcagaatctc tcatatgaac
     1381 cttcttaaaa aatactgcta aatagattcc aatcactgct ccaactatta cacctaatat
     1441 aattgatact ggaatttgta aaaagctaat agctgatata ctatttcctt gtgccaatcc
     1501 tgtgaatact gtaaacatca caattacgaa aatatcgtct atagaagttc ctgctaaaag
     1561 catctgtgga atactttttt ctgtaccata accttcttcc attaatttaa gcattttagg
     1621 tacaataatt gcgggagaaa ctgctcctac tactgcaccc atgactgctg cttccaatat
     1681 agaaacgcct agtaactttg gtgctaaaac tatcattccg ataatttcaa aagtagctgg
     1741 aataaagcac ataagtacag cgggtcttcc cactttcttc aaatcgttta tatctaaaga
     1801 taatccagcc cttgttaata taataatcaa tgcaatcttt ctaaggtcag aagaaatatc
     1861 tagtattgaa ttatcaatca aatttaaaac atatggacct aatataattc ccgtaagtaa
     1921 cattcccaat aagttaggta attttatcct cttaaaaata cctcccgagg ccattcctaa
     1981 taaaaaaatc aaagctaaac ttgtcaacat attcctaccc tatcctttct ctaatattct
     2041 ttctcttcta atcatacatt atgttgtttt atttattgtt ttgttgaaat tttttgtagt
     2101 gaataaatag atttatccat attaaatata tatgaaatag cgcatactac aaagaaataa
     2161 ggtaaatagc tgtacccaaa aacttctgcc cctataaata caggtgcaaa aaaagtattt
     2221 gtagcactac caaagacact agcatagcca agcgctgcaa ctaattcaat tggcaaatta
     2281 aaaaatcctg ctaacaatac accaagacta gctccaatgg aaaacaaagg tgtaacctct
     2341 cctccttgaa aacctgctga caaagtcagt attgtaagta taaactttaa cagccagtca
     2401 aatgaataaa tttctccacc atagaaacta ttttgaatta aatttgtccc taaacctgaa
     2461 tatcttcctt tataaaataa aaggaataat acactaagac atactccaat aatagcaatc
     2521 cttatcatag gattttttaa tctattccct atttttcttt ttgacaattt cagacaccat
     2581 gcaaatgctc caccaaccat tccaaaaata attcctaaaa caataagctt ccaaaatatt
     2641 gacaagtcaa atacaacttt agaagaaagt gcaaaagaaa atttctctaa ccctaaagct
     2701 tttgatactg cacttgctgt aaaagaagca gtaaacgcag gaaaaagcga ttgatactcc
     2761 agtgaaccag caaccaaaac ctccatagca aatagtattg ctgctattgg tgtctcaaat
     2821 aatcctgcaa aaccagctgc cataccagtt actagaaaaa tactagaagc attttttata
     2881 ggcaaccttt tacctaccca atgtgaaaaa gttgcaccaa tctgaacagc aactccttct
     2941 cgtcctgcac taccaccaaa taaatgggtt aaccatgtac ctgatattat aaatggaacc
     3001 aatcttaaag gaataatttc ttcttctcca tgccctacct caaatattag attcattcct
     3061 ttactacttt ttcctccaaa tttaaaatag caataagcaa taacaactcc tactactgct
     3121 aaaaatggta tcagatacat tggatatgtt tctcgaatat ctgtaacttt tagaagaacc
     3181 gttccaaata tggtatctat aagacctata attgcaccaa ctggtatacc tatgagtcct
     3241 aaaaaaaata gtccaccata tgttttataa acgcttttaa ttcttattat cattttaatc
     3301 catattaaag ttttaaattt ttctatcatt ttaattcctt tctatttgca tcataagatt
     3361 ttttgaaagt tttattacat caactgtagt caatgtaatg actatcataa ttgtttttta
     3421 aattgatagg atataggtac actcttaacc cattcttaat aaaataatta acatataatc
     3481 tactattttt tctattaatt aaatatttct tcaaatttaa aaaataaaaa tttagtaagt
     3541 acttcacctc cattttatcg atttaaacaa caaaaaaagc tggtggcatc tatgatatca
     3601 tagaagtcat cagctcattg gcagtttagg atattatcat ccaagggaga acctcattcc
     3661 cataattatt atataatttt ttatttttca tatgtctatt ttatgtaaat tgtattttta
     3721 atgttataaa taatataacc tatcaattaa aatattaaag tctctaatat tatgagtgtt
     3781 caatacttca tattattttt atgatatata atttaaatga ttttctttac tttgcattat
     3841 gatacatgat ttaaatattt ttcttcactt tgtatactaa agtttattta aaatattttc
     3901 tttcctatta tactatgctg ttaaatattt ttcaactgaa gtattgtaga actttaaaat
     3961 tactataaaa aattatattc taaaatactt gtaatactac atatcaccct taacacttaa
     4021 gtataatttt aaagcaaaat tcttattact tatatttaat tttgattcta catctttaga
     4081 tataatttta agtaatttaa gaaattattg taaatcatat agtactactc aataattagg
     4141 tttttcaatg ttccaattat gtttctttga tataatccac catgactaaa aactatcgtt
     4201 tctatatcat aattacttat atttttaata gcatctttta actcttgttt gttgctgtac
     4261 atcacatcaa caggcttaag ttctccattt tctacctgta acaaatctcc agcaataagt
     4321 actttagact cttttaaata aagacatatg tgacctctag tgtgacctcc tgtatgtata
     4381 acttctattt cctcacctag attcaatttt tcatggttat ctaatgacac atctacttta
     4441 gtatatgatg atttaaatcc aagtcccata acatttagca tactaagcat cttatcatca
     4501 attttatcta ccatttgttc caacatataa agtttaaaag gagtttcttc tcctgttata
     4561 tagctgattt cacttttgta tgcatatact ttgatattat tcttagattt ttctcttaga
     4621 taatttatgt ttcctatatg gtctatatca tgatgagtga ttattatatt ttttaactta
     4681 tcaaagctcg tgttactttt attaagagca tcaattatta aatcaccttg acccggaaga
     4741 cctgtgtcaa taagtgttgc atcatcttct gtgtagatta caggaatata cataacttta
     4801 tcttcaccga atacatttga tgaaattttt aaaacatcta atccctttgc taaattcata
     4861 atttttctcc ttataattac ttaattagag gtcaatttaa ttaaagaaat tgagaaaatg
     4921 tagattttcc tctagttata ttttattaat taaaaattat taatacctag tatatcattt
     4981 aagttgaagg attactcgac ttgatttttt tgttatttta tggtatagta attaatatag
     5041 aagtaagtaa ttgcaagctt tgcaattact tttatttttt tctaataact attttatttt
     5101 tgataattcg tataaaatta aaagtaaagt aatatagaaa atatgaatat agaaaaactt
     5161 taataagaaa ataaattatg aataaataaa agaaacgcaa tactgtatat agatgattat
     5221 ttagaaaaat aaaatctctt ttaataaaaa taagcttcaa aataaacttt tagttcataa
     5281 agcttaggag taactaaatt gagtgcaagt gtatctactg ttcaaaataa atagttcata
     5341 aagcactctt ttttatatta aatcaattat attttcgtca tttcagcaat gaaaaagaag
     5401 cttatctcta ttttgagaca gcttcttttt attaacgtcc tactttttat tagttttcta
     5461 aaattaaaaa gctttattta taggattcct tagatttata ttttgacgat ataatttttt
     5521 aaaaacacct taaaagatgg tatacaatac acacaatcat actcataatt gaaaaataat
     5581 caatggaaaa caaaattact ggatattccc tattaaaaaa tacaaattga ttcacaagaa
     5641 acatataatc tataatatta tgatgttact ctcccacacc aagtaacttt attttcaagt
     5701 aaccttttca taccatctaa cttcactcaa taaaaatggc aatataaata tatactctat
     5761 tttttacttt taaatatctg acaagcttta tatactattg gaagtaagat acaaagtaaa
     5821 acagcccaat ctggtaaatg tggcatataa aagtccccct taccttatac aagctcctta
     5881 ttactaaaaa ttttaataga aataaacatt gaagctaaca gtataattaa acttaaaagg
     5941 aacaatacaa tattcactat catggatgga tactgatata taaatggaat ttcaaaattg
     6001 ctgtgagcta tagttaatgg taatacaaat gatgaagcca tgattatcat cacgaaaaag
     6061 aattttgttg tttcatatcc taatttgtat tgaataggca tataaactcc ataaataata
     6121 gaacttatta ataaaacaat caaaatatat gtcaagtcaa aagttcctat ttgtggaata
     6181 agtaaatttt caatccagta aactaatgta caatagataa ataccattgc aaataaaaca
     6241 tacttagatt gaactaagcc acgtctagga taaggagttg aacaaagtaa tacagatgct
     6301 tttgaatatt gactctcctt taataataga tactgacaca gcataaattc tgaaaatata
     6361 attgatagta caaatcccaa tatttgtgca aatggaggta aacgccataa tataaataat
     6421 gggattccta ttgcaataaa taatatcaac aatacatatt tttttacaat cagaatatct
     6481 tttttaacta aatgaatcaa cataattact ctctcctttc aatatatgca agcataatat
     6541 cttcaattgt aggacgctct attaaaatat ctggcatatt tttttgcact tcattaattt
     6601 ttgaggtaat ccctataaaa ccataacctg taatttctaa atttaaaaat aattttttgg
     6661 tttcatcatt tagaaattga atatcccctt ttacttttct atgttcgtcc aatagagtat
     6721 ctttttcacg ctctaatata atacgtccat tgtcaatcat aacaatcata tctgctattt
     6781 tgtctaaatc agatgttata tgagtggaaa agaaaacacc acgaccacat ttcttcatat
     6841 atcctgttag tatgtcaagt atctgactac ggattagtgg gtctaaacca cttgtaggct
     6901 catccataat taaaaactca gctttatgcg ataatgctaa tgctaaagca aatttcattt
     6961 tcattccttt tgaaagtgta tcaatctttt gttttgggtt aagattaaaa cgcttcatat
     7021 tttgttgata atcttcttca caccaattat catatgaagg tgcaataata cttttcattt
     7081 ctgccattga caactcctca tagaaatatg aatcatcaaa gacaactcca attcgatttt
     7141 ttaattcctt ctcatttcca tcgaaatctt taccaaagat ttttattttt ccactatcac
     7201 gctttacgac atccaatatc gaacggattg ttgttgtttt tcctgctcca ttaacaccta
     7261 taaatccggt tatacaatct tctggtaatg caaagcttat attagataat gaaaaatcgg
     7321 tataagtctt atttaagtta taaacttcta aaatattttc catattaatc ctcctcatat
     7381 agaatatcca tcatttcttg taactctatc tgtgtaattc ctagtgcttt tgcatcttta
     7441 atcatatctg ccattttttg ttcaactata cgtctctttg agtctcttag tatttctaaa
     7501 tttccagttg aaataaatgt tcctattcca acttggcgtg taagaaagcc ttcattttca
     7561 agttcttcat acactcgtct aatagtcaaa atactaactt ttaaatcatt agcaaaattt
     7621 cttatggatg gcattgcatc cccttctttc agttcttcag taaatattgc ttctttaatt
     7681 tgctctttaa tttgttggta taatggctta tctgatgtgt tagaaatgag cactttcatt
     7741 ttttatcacc ccctttttat cactttcagt gtgatgttat catatacaca ctatacactt
     7801 agagcacttt tttgtcaata taaaagatgt aaaatattac tatgacaaaa taaatatgaa
     7861 tgaaatatta gagattgtta atagccataa acacttttta aaactacata agataggatg
     7921 tattaaatat ttattaaaag taagaattga atatataaaa gaaaaagtaa tcctgtatga
     7981 atgatttgtt taatgataaa ttaagttata aactatttat aaaaaacata aaggaataaa
     8041 tccatttcta attacaaaca tactaaaaaa tttagataat aataaataaa tagtaataaa
     8101 tgggatgaat ttataagaat ttggatagag gaaagagtta ttcaagagtg tagactagag
     8161 gtaaactcta tattaaaaaa atttaaattg ggatattatt atgtatggaa tatttgcaga
     8221 agtaaccttg ctataagtat agaagatttt tttgattaca aaagataata ctctaaaatg
     8281 tgaggatttc aacattcaat actgtgcttt aaataatatc actccaaaat accatccacc
     8341 ttttactata attgaatacc ctaattattt taaaaaagaa aacattagaa acggaaaacc
     8401 ttgtatatat aaaataaaaa atactaactt ctaaaacacg aatactcttt gataaataaa
     8461 acaaaaaata attttaataa ttaaaattgg ctatctcaaa ctagagataa gtctctttct
     8521 cacttttaaa acgttgaaaa tatattcgat ttcgtacaaa aaagagtgcc ttagaactaa
     8581 aaagttcact ttgtggcact cccttttttc tcctcttttt aattttttat aaaaattctt
     8641 caaaaatatt attaaatacc ctatattata cataaatcaa attaaagttg cattttagca
     8701 aataaaaaca aattataact attagcatag caaataaaaa atctgctatc ttataaaatc
     8761 aaaattataa ttataaaaag aaaatattct tataacaaat taactaatat aaaattattt
     8821 tataaattaa tactactttt tagcttctcc tataaattaa taccactttt ttaactatga
     8881 aacctatcta caacattggt accataagac cagctaccac aatagaaatc atacaagcca
     8941 taccaccaac taccagctta ggcataatct tagacatcaa atatgctctt tcttcttccg
     9001 tttctccaac agcattacac acttcatttg aaattaaata tgtagttggg aatcccaaca
     9061 tctgacaaaa ccctactcca aaagcaagag gtttagaacc gataattttc caacctggta
     9121 acaccttcat cataaggaat ataccaatga tagaagctgc aaacataccc acaattggta
     9181 caaaaagact aattaaatct gtcaaagaaa cttttgctaa agaaggaata acagctgcaa
     9241 acacaaccat gttgataaat cccttgattt ttcctttttc caaaatgtct tttggcacaa
     9301 taccaatacg tgttacaata acaccaacaa caagacatac aatagaataa tttacatgaa
     9361 taatctctga aagttcatac cctagataag agaataatgc tataaaaaat atacaaacat
     9421 ttgtagaata atatttatcg tatttttcac agaaagcttg ttttactttt gtgcctgtat
     9481 tctctgcttc tttcttatca gtatcaacat ttgctaaatc aatgcctttt gattttgctt
     9541 catgatattc tactaaaaga cggtttgcct cttgtaacgc tgctcttgaa gctattggtg
     9601 taccaataaa cttctgaatt gcaaatacaa ctgttgctgt agctgctgca agtgtcagtc
     9661 ctttttctag tgctgcttgt gtcataatag tagtagcagg taaagctcca tttataactg
     9721 gtatgcttgt caatactgtt gattttccaa acaaaggaat acataatgag acacagacta
     9781 taactgctac aattccaagc catgctgtca atactgttct ccattcgtct ataaattgtt
     9841 taacattcaa cattgttccc atactaaaca taatcattgg aacactccaa ctagctgctg
     9901 cggtcatacc tgctttttca ataatatcag ctggtattgc ttttgtgaca aatagaatca
     9961 aaaataaaag tagtgtaaca aatacagctg atacttttgc ttttgttttg cttgcaatta
    10021 aatccccaat agcaaataaa actaatacga cactaaacca taataacata tcactcatag
    10081 aataaccccc ttattataaa tatctttcaa accaatctac aatagcattt gctcttgcaa
    10141 ttctattaga tggtcttcca tttctagaca atccatgtgt atctccatga aacaaataca
    10201 tttctgtatc tacaccaagc tgttttaatg ctgtaaacat ttggaaagta tcccccataa
    10261 aacatctata atccctatca ctatgtaaga acaatgttgg tgtcttaaca tttttcacat
    10321 atttaatagg tgaatgattc caaagaacct cctcatgatt ccaaggcgtc ttatctccac
    10381 aagtctgctt cataattcta tccccaaagt caataattaa agaagctgta atcaagttac
    10441 taatacctct ttgagaaact gctgctttga aacggtctgt atgaccaatt atccagtttg
    10501 tcatataacc accgtaactt ccccctgcaa cccctaagcg gtctgcatca atcccaggat
    10561 atttttcaat acaagtatct acaaattcca taagatgctc atagtcaaca tacccccatc
    10621 tttctaccat ttctaagaat ttttcaccat aaccgtcacc accatgaggg ttacaataaa
    10681 tcacatacat tccctttgat gttacacact gcttcaagtg tgtaaacata tagccatatg
    10741 cccatttagg cccaccatga atgaacaata cagcaggata tttttcgcct tctttagcat
    10801 ctataggtgg tataacaaaa ccatcaactt cttctttatt ttttgctaca aacgtcaatt
    10861 tttcaggtaa tgaaacctta tgagtatcta aatatggttt agaaaaagca gtcatttttt
    10921 tcattgtacc tgtagtcata tcaatgctat atatttctgc taactcataa tctttcaaca
    10981 ttactgtgta catggtgttg tcgtaaatat caaaagatgt aaaagcatct acttttgtga
    11041 tgatacgtat attacctgtc tttgtatcaa actcattaaa ttctgtcata gttctgcaaa
    11101 ttcttgcacc aataaatttg tctcctataa ctttattact acgtccacca ccatatactg
    11161 catctacatc cagcaatgta gcatacatac catctggact agcaatcata tttatttcat
    11221 tagtactcaa atcaagtata aaaatatcat gattctgtgt gatagaacgg tctaatgttg
    11281 aagcttgcaa cattacttta tctttgccaa tatattttgc caaacttatt tggtactttc
    11341 cttgtggtac aagttctttg gtttcaccac tgtctatatg atactcccaa agtccagaaa
    11401 aaggagttgc acaatcttca taaatctgac cagtatataa aattctgttt ttatcagaag
    11461 aaacgtctac acctgctact tcacaaaatt ttggtgtaat ggcttttgtt atattttcac
    11521 cttcttgata taattctaat gttacacgtc ttctttgaga aaaattttct ccatctctta
    11581 taaaaggttt atctgtaaat gtccaataat ctacaccctc tactgcccta tctggttctg
    11641 attttttaga atccgttgga ttttttgttg tcagtaacca caatttattt ccaagtgata
    11701 caggtacgct acaagctttt ggaatagaaa atgctttttt tgcttctcct ccttctatag
    11761 aaatagtgta aaaatctgtt gtacctgatt ttggcttatt tcttttagat gtaaaaagga
    11821 gttctgtatt agaaatccaa ctaaatgatg ttacatcttt tgtttgagtc atatgtattt
    11881 gtttttctgt tttgatgtcc atcacatata agtcatgttc atagcagtct ttttcctcgt
    11941 tgatatttga tataatataa gcaatatgct ttccatcagg tgaacaagaa atatcatgag
    12001 ggaatatata ttcaaaaata tctgaatttt caatctttat tttttccatt tttatattct
    12061 cctttctact gtcctatgtt atcttttttt atatatactg aaaaccaatc tagcatttca
    12121 actaatcttt gtagcttttg ttttggtcta ccagaacgag acagttcatg gttttctcct
    12181 tcaaatagac aaagttttgt aggacaccca tgaattttca atgctgaaaa catctggaat
    12241 gcttcttgca tatagcatct atagtctttt tcaccatgta aaaataaagt cggtgtaaca
    12301 catttatcag cataagcaag aggtgactgt ttccattgaa gctcgtggtt ttccatatga
    12361 gaagcacctg tttgtccttt gcagaaataa taaccaatgt ctgttgtacc ctccatgcca
    12421 ctccagtttg caatagaacg ctgagcacaa gcaacagcaa aacggtttgt atgaccaatt
    12481 atccaattcg tcataaatcc accataactt ccaccttcaa cagccaattt tgttttatca
    12541 atgtctgtat atttttcaag tacagtatct gtaaattcca taaaatctat ataatcaata
    12601 tctccaaact gtttcctaat atctgcaaaa gcattgccac gaccttcaga cccccttggg
    12661 ttacagaaga atacaaagta acctgcactt gtaaatacct gtatctcatg cataaaatat
    12721 ggtccatatg tcaatcttgg tcctccatga atcgccaata cagctggata ttttttcccc
    12781 acctcataat ctactggttt atatacgtat ccttgtattt cccatttaga acttcctttt
    12841 gactctatgt attctggcac agaaatttta tattcctcta aaatatggtc atttaaatgt
    12901 gttttcttac acaatgttcc attctctact gtataaactt ctggtaatcc atttcctaaa
    12961 caacccacac atacaatatt gccatctttt acatctataa aatcaactga acctgtctgt
    13021 gttacttttt cctgttttcc tgttggaata tgtattcttt caataatgca gtccatatct
    13081 actgtagaag tgtaataaat atattctcca tctacctttg ttgtctgacc agcacccatt
    13141 ttaacatcag atgttactgt atttccaata caatgatgtt gatgccctga aagaagtttc
    13201 atttctttcg tatcaatatc aatagtataa aaatctccat tttctccatt tccataagat
    13261 aaagcatcag ttaggtttag tatcacttga ttttgatata attcaaaacc ttttataata
    13321 tacttatctt tttctaatat acagacattt gtgtttgtgt ttgtatcaaa tacatagacc
    13381 ccatttttca aaccttttac atctttaaac tcttgcccta tatataagat ttttccatca
    13441 cttattttca tacctacaac attaaacata ggtgctgtaa ttgccttcaa ctcatttata
    13501 ttactgtcgt atacatatag gcgttttctt tttctgctaa tatatccctc cccattgaca
    13561 gcataaggaa tttctttgaa atgttcataa tgtcttttgt tataagcatt tatttttttc
    13621 tgttgttctt cttctggcaa tccctctaca ataggtcggt cattgtcata agaatcaata
    13681 actgcataca aaccttttcc tataggattc aatttaccac ctttgatagg gagtctaaaa
    13741 agttcttctg cttctccacc atttaaagat aaagcatagt aactcatgca ttgcttaccg
    13801 tttttgacag cttcttttac tttttcattt cgcaaagcag ggaacaagat agtattttca
    13861 tcttcccagg catatgctcc aacagagttt atagacgtta tttgttttaa ttcgccagtt
    13921 ttattgttta atatataaac ttggcttgtg taaccattct ctttgatatc agcttttttt
    13981 acaataaatg ctgtatatcc tccatctggt gatatttggc aatcacttat aaattgatat
    14041 tccaaaaatg tttcactcgt aaatagcttt ttcatagtat tttctatcct ttctttagta
    14101 atttttttca aactctctac cacctctttc attttttatt ttaaatttaa aataaaaaat
    14161 tttaaattta aaataaaaaa ttttataaag gttatttttt taggaagcct tctggcttcc
    14221 ttgtaatatt tgtaatttaa agtatattta atccatgctt aattcatgct taattcatac
    14281 ttaatctata cttaatctat acttaatcta tatttaattc atacttagac ttatttacac
    14341 tatatttaga ttatatttat tagattatat ttagattatg tttaaactat aattagatta
    14401 tacttagact acatttagca acttaataga taagtatata tagtaacttc aaaacttatt
    14461 attcatcttc acagaatctt ttaatagttc cttgaatatg atttcttagt ttatctactg
    14521 ctttttttgt atctctgtct tcaatgaatt ttaatatttc aaaatggtct tgtactgttc
    14581 cttcataatt acgatttttt aatggtcgaa ttaaatagtt cactactata aatcttcctt
    14641 gtataccaac atatacatca tttaaaatct cattatttgc cccttctaca atggctttat
    14701 gtaattccaa atctgctagt gcaagttcta cagatttttc ttgacgatag tactcctcgc
    14761 atttatcaga gtatcttttt attacatcta gcttttgttg aaaaacctct tcctcttctt
    14821 gcatcatttt ttctaaagca tatgtttcaa tcagtgttct cgtttcacaa acttgtttta
    14881 ccatatcata ttcatactct ggtacgtaca ctttaccatt tgtcataata gtaagcactc
    14941 catctttatt tagcattttt actgcttgta caactggtgt tctgcttact tcaaattttt
    15001 cacaaaggtc atttagtaat attttttgac ctacagagta ttcaccacga ataagacttt
    15061 caataatctt atgatataaa atttcatcta aatttgactt tactttcact actctcacct
    15121 tatttctatt cttttgccat attatattga attactggac tttaattact tttagtaata
    15181 tttttaaagt agaaattctt atttgatagt acttttgtac tacaatttaa attattgtac
    15241 tgtaaatcat gctcttatac tatagattaa ttcattgtat tgcaaatcaa gttattgtac
    15301 tatagattaa atcgttgtac ttcaaattaa actattatac tatagattaa accattgtac
    15361 ttcaaattaa actattatac tatagattaa atcgttgtac ttcaaattaa actattatac
    15421 tatagattaa ttcattgtac ttcaaattaa actattatac catagattaa ttcattgtat
    15481 tgcaaatcaa gttattgtac tatagattaa atcgttatac ttcaaatcaa gttattgtac
    15541 tatagattaa atcgttgtac ttcaaattaa actattatac tatagattaa atcgttgtac
    15601 ttcaaatcat gctagtgtac tatagattaa atcgttgtac ttcaaatcat gctagtgtac
    15661 tatagattaa atcgttgtac tttaaattaa actattatac tatagattaa ttcattgttc
    15721 tgcaaatcat gctagtgtac tacaaattaa actataaatg tttactatct cttaataatt
    15781 tgaataatat gttacaaaaa gtatagcacc tttttatttt tttcgcaata aataaaaaat
    15841 ttaaaatttt ttatttttta ttttcttata ttatcaattt gaaatatgaa agattaagac
    15901 taatagaatt gattcagtta attgaaaaaa ataggtacct tttatgaaaa ttctaatgat
    15961 aagcattttt ctgatgtaat ggtaaattat aaatgataaa attaaaaatc acttttagtt
    16021 ttatgaactg tccccatatt aagaaacaac taagatacat aaaaatagtt ataaagagat
    16081 agttaaacta tatccactat tttatatagt taaaatagtg ggtatagtcc aagctaagaa
    16141 taactaagaa attagcgtta aatagcatag ataaaaatat atttatataa aaactttttt
    16201 tgctgcaaac aattatttcc ataacatttt atatacttct ctaaaatctt ctaaacttga
    16261 taaatattca attaaaggga cataattacc taaactacaa gaatttaagg agtttctata
    16321 caattcctca ctatcccaag atacaatttt tataggaaga ttattttcta acatctgttt
    16381 tagcataaca aatctaccta tgcgaccatt accatcttga aatggatgta tcaattcaaa
    16441 tctataatga aattcagcta taactttaat agatacttca ctctgactat agtaccattc
    16501 aattaattca tcaagctttg gctcaacttc aaatggttgt gcaatattat taaaactcta
    16561 ataatattaa taatagctga tataatagca aatcattatt atcccaagaa gatacaatta
    16621 ataactaccc tcctatccac agtatctagt caatttcagt aactttatga tttagtatat
    16681 aatatatacc aatcacagtt ataccaacta taataaaaac agaaatcaaa ctgtcttgag
    16741 ttgtatttgt accagctaaa ataaattgtc ttaaattgat aaaaataatt gaactgacaa
    16801 gggtaacagg tattgatttt cttatcattc caacaacgaa tgtccatata cctaatgagc
    16861 aaaatacaat aatactttta attgctcctg gtatccaata agataaaatt gaaatatgaa
    16921 aatctccaat aactaaatta aaatacttat ccaacacaac aacgaatgta aaacaacaga
    16981 tatatccaac tatcattgat gatatcataa gagtcgtaat caaaagtaac tttgataaaa
    17041 taagtttttt acggtcaagt ggataagaga ataaaatcaa gattgtttta tttgtatatt
    17101 catttattat atatgccaca ttcaacacag caaataatat aatatagtaa aaacagaaaa
    17161 ataaactgat tgtcctaaat gtaacatcat aatcatatat tgaagtagat ttatcattca
    17221 atccagtaaa gacaaaaaac atacataaga aaatccccag cattgaaaat aaaaaatatc
    17281 tttttagccc aacttttttt agctctagtt tcattaaatg tatcatagtg tttctttacc
    17341 tcctgtacct gtagtttgga agaagtaatc ttctaaagaa ctttgtttac gtccaatgct
    17401 atttatacca atttgatttt gaataaatat tttggatatt tcttttacat cttttcgtaa
    17461 atcatatatt tcaatacaac tttccgaagt aattttaaag tcttttattc caaattcttt
    17521 ttctaacaag tatccagcaa gctccacatt atctacatct acttcaacat aatctgtttg
    17581 atatttgtgt atatctgaca tagaaatttc tttaataagt ttaccatctt gaataattcc
    17641 aatagtgtcc gcaatttgtt ctacctctga aagaatatga ctagaaataa atatagttat
    17701 tccataatct gtatttaact tacggaataa ttctcgcatt tctcttatcc cctctgggtc
    17761 gagtgcattt ataggttcat caagaatcaa taattctggt ttagtaagaa ttgctctagc
    17821 tattgcaaga cgttgtttca tccctaaaga atattttgaa actttcttgt ttgattgctt
    17881 tgacaatccg acacattcca atacctctga tatatgttct ttgttataat aacccatata
    17941 ttcacagtgt aaatcaagat tctgtttagc agtcaaattc tcatataaat acggattttc
    18001 aataatagta ccaatctttt ttaatatttc aaaatcagtt tctgccactt tcttaccgaa
    18061 cataactatt tctccagaat ttggattaac aagatttagc aacattttca ttactgtact
    18121 tttacctgcc ccgtttggtc caacaaatcc ataaatttct ccctttttca catgaatatt
    18181 aacagcatta acaacagctt tatctttata tattttcttt aattgatggg tctctatgat
    18241 atagttcatc atactccctc ctttctaaaa acagtataca aaataaaatt gtcttttctc
    18301 tcttttaaat ctaacttttt tctaactttt caattttacc gtaaaaactg tttttacatt
    18361 aggtctacta tttacataaa ttgaacctcc catagagttt gcaagccctt gagaaatagc
    18421 caaacctaaa ccattgcctt tttttctgtc agcagtataa gctcttgtga aaatatgctc
    18481 tttttcagtt tcagaaatac ctgaaccttt atcctcaata tctataaaaa catgtttttc
    18541 cttttcatat agtgaaatcc ctaaaaatct accatctgaa ccatatttta tagcattatc
    18601 aattaaattt ttgagtatac gttgtaatgc ttctgtgtca acatttgcat atataggtct
    18661 atcttcaata ttaacttcaa cattaaagtt ttctttatct agcaaatcat aaaactctaa
    18721 aataatctca tgacataatt gtgttagatt ggatttttga agatttaata tcatatctcc
    18781 agattggatt tttgccatat taaacaggtt atttattgaa ttaacaagct catttgaatt
    18841 tgtttgcatt ttttccacaa ttgctctcat tgaagaagat aattcttctt tttgactttg
    18901 aagatatagc atttcaatat aaccctttaa tactgtgatg ggagttcgta aatcgtgtga
    18961 tatgtttgta aatatttgca atatcgcttt ttcacttcta ttataattaa tttgcctgct
    19021 gtaaaacttt tcaacaagtt tatttatatc tactgctgtt tcttttacaa tcgaaatatc
    19081 agatggtact aaaatataat taatttcatc atcatttgtt atgtcacgaa gtcttgaatt
    19141 gatatactta taatcacttt ccattttctt tgatttattt cgttctttta agtagagagc
    19201 aaatagtaca atcgaaatta caactaaaaa taactccatt attcttcctc cattttgtag
    19261 ccaattcccc aaagagtttt aatataatta tttttttcta taccatcaga caatttattt
    19321 cttaacctgt taatatgagt atttagcaca ttttcatttc cataataagg ttctttccaa
    19381 atagcgttgt ataattgttc ctttgaaaaa gcacgtcctt gattagtcgc tagtaattta
    19441 acaatttcaa attctgtatg tgttaagttt aagtcaattc cattctttga aactacatat
    19501 tttgatatgt ttataatcaa atccttatac cgaataacca tatcttgaga gtttcttgaa
    19561 ctgctataca ctgttgcacg tctaatatta gctttaattc ttgcaagcaa ttctacgatt
    19621 gaaaaaggct ttgtaatata atcatctgca cctagattaa gccctagcat tttatctgtg
    19681 tcactatctt tcgcagtagt aataataaca ggaacaacac tcacacttcg tatatatttt
    19741 aatacttcaa aaccactaga ctttggtaac atcaagtcca gtaaaataat atcaaatgaa
    19801 taatttttaa atatttcaat agctgtattt ccatcaatgg cagttttaac agagtaattc
    19861 tcactaatca gaaaatcgta taccatttct cgaatattct tatcatcttc aaccaataag
    19921 atgtccatta ttattcaact ccttttcgtc agtatacttc aaatgatata tttattaaaa
    19981 agggttgtca ataaataact gcttatgata ttgtatctgc aatttggtga tactcttcta
    20041 agttaaagtt cttttcaata agctttattg aaaaaagaat taaaatatag tatactctct
    20101 tatctttatc taaaatatac ataattttct tttattaaca tctacaatta actattttat
    20161 attataaaac accttttaac ttttgctaaa aaatatttta tacataaaaa agaaaccaca
    20221 ttctagtttt atacagaaca tagtttcttt aaaaataatc aaatttggat tttaaataat
    20281 taaaaacaaa cttttatttg tttttaattt tccaatttta tagtagataa tttagttaat
    20341 tagttcttaa atgtttatct tctttagtaa atttttttct tattaagaaa tatgcaacta
    20401 ttacaacaag ttctattgct aaaaatacac cataagtagc tatagaatct atgtatctat
    20461 gccatttcta aagaaaatta aagcaattcc tgctgctata atacctacag cattttaatt
    20521 ttgaccaaaa ccataaaaag aagggaaatt tccttttatt tcaatgaatg tcaagattaa
    20581 tccaatagct ataaatagta ctcctacaac ttccataatc atgctaggca ttttgacccc
    20641 tccttattaa taattaacta ctactataaa aatatttatt atttttataa ttatattata
    20701 tatgcgatta catgtttttg gaagtatatt tggaagtaat ttcatattta tttaacttaa
    20761 tttttttgtg ataaaataat tctaattttc atatagatat tattataaat tttctagttt
    20821 atggagttat tcattctata attcacaaat atgtattttt taatctatta aactattcat
    20881 tttctggctt ataagcatgg acttcttatc tataagcttg gtttttcatc taataagtta
    20941 tttattcttt aatctatgag cttgattttt catctataag tcatttattt tttaatctat
    21001 aagtttagtt cttcatctat aaatcattta ttttttaatc tataagtttg gtttttcatc
    21061 tataaataag tcatttattt tttaatctat aagtttagtt cttcatctat aaatcattca
    21121 ttttttaatc tataagcttg gtttttacct ataattaatt cattctctaa tctataagct
    21181 tggtttttat ttataagtca tttattctct aatctataag cttggttttt atttataagt
    21241 catttattct ctaatctata agcttacttc ttaatctata agtcatttat tctctaatct
    21301 ataagcttac ttcttaatct ataaatcatt cattttttaa tctattagct tgttttttac
    21361 ctataattaa ttcattctct aatctataag ctttcttttt catctataaa tcattcattc
    21421 tttaatctat gagcttggtt tttcatctat aagtcattta ttctctaatc tataagcttt
    21481 ctttttcatc tataaatcat tcattcttta atccattagc ttggtttttc atctataagt
    21541 catttattct ctaatctata agcttacttc ttaatctata aatcattcac tctttaatcc
    21601 attagcttgg tttttcatct ataagtcatt tattctctaa tctataagct tggtttttta
    21661 cctataatta attcattctc taatgcataa gtaatacatg aaatttaaat attattttct
    21721 tcaataaatt caaacaactc ttctacagtt gtatcaaata ccttagcaat atccattgcc
    21781 agttttaaag aaggattgta gcgaccattt tctaaatgta caatagtttc acgtcttact
    21841 cctactgact ttgcaagttc accttgtgtc ataaggttct tttctctata ggatttaatt
    21901 tttgtcttta gtaatactga cacctaattg tatatcccct ttctgtcaaa atatataaat
    21961 agaattaact tcaacaagga ttgaataaac aaaatgacta ataaagacat tcctatattt
    22021 aaatttgaca catttatgtc caaagttaca agaaatatag atactatagc tacagaaaaa
    22081 aacaatactt ttattgcaat atcattaact cttgatagta tacattttga tgcctcatca
    22141 attaaatcat catacttttt attatagacc cactgagctc ccatagctaa tacccaaata
    22201 attataggaa gatattcaat actacttcct ttaaaaaaat acttatttac taatgtagct
    22261 ccaatacaat aagaaattcc caatatttct gaaataagtg ttgatttaat accagtttgt
    22321 tgtttagaca tataaaatcc cccttaaaaa gttatgtatt tataacacct cttgttataa
    22381 atatataact tttttgaata aatttcaata aaaatattat tttgcaacaa attcttttat
    22441 taattttaca ttagatagag gcactttaga tgtcactcct tgcaatgtca aatcttctat
    22501 attaacttca tttaattttg aacacagttc taagtcaaat attctttccc aacttacctt
    22561 cttttcaatt tgttcatctt cataaagctc tacagtaata tccattaaag gtaaatgcca
    22621 taaatcaaat tctgataata acacattgtc ttcacttatt tctatttcaa gtaaaacacc
    22681 tttttttcct ttttctaaat aaccactaca tcttaaatca ggtctcttcg tccaaaccca
    22741 tatcatacta tcatcaaatt catatattct atttgtcatt tgactataca tccatttgta
    22801 tggaatatca aagtcttcta atataaaaga ataatcattt gatagatatt tattgttttt
    22861 attgttattc caaaattcta aagtctgtat agtataaaat ttcatatata aatcaacccc
    22921 tttgtttttc tataagaagg agatggttca aacttttatt ttgaattatc tcctttattt
    22981 atacacctct ttgtacctat tatttatata tctctttata tctaccatat ttaaataaca
    23041 aagttttata ttaatttaaa tttaaaagag gaagctttgt atttatctag tgccatctca
    23101 atcatagtat tagttttgat tatagacttt ttcaacctct gatttattca aatcatctat
    23161 aatatcgcat atttcactca aattgtatcc tttatttttt aattttatga tgatattaaa
    23221 tttctcttct cttttccctt ttgcaatacc tttttcgata cctttttcga tacctttttc
    23281 aataccttct tgaaaaattt cttctttaaa atttctactt ttattcataa tatacgcacc
    23341 tctctttaaa tttaataaat agtcctcatt actacctaat tcttttaatc tatcaagtgc
    23401 tcttttaata acataatccg ttttagatag tattctaata tctatatgag aaggattatc
    23461 aataagttta ccccatatta gaactctgtt tgctatacaa ctggatttac gaatttttgg
    23521 aagttctata aaatataatg cagttttact agaaaaaata tctggatgcc cgtccattgt
    23581 taatctcagg caatttacat aatcttctat gtgattatat tttacatgat ttagtatatg
    23641 tattccaaca gtttttccaa tttctttata ttctttacct tcttctaact ttctaccatg
    23701 tacacgacta agataatatt cagctctttc caaataatcc tcttcaagag ctctttgcat
    23761 ctcaatattt atatggttac gttcttcatc ttcagccaaa atatcaagtc taggtctttt
    23821 ctcaccaatg taattaattg ttgggtcggt ttcaacctta attattcttt ttatttgagt
    23881 gaactgtttg atattcctat tagcatatgg agcatctttg aggatatcat ttataaaatt
    23941 tgcaagtaaa tcgtaatcat tatttaaagt aaacaatgat ttaaatacct catcatactc
    24001 aggcttagca tacccatctt cagttacaac ttctccaaca ttttcactag tcaatatttc
    24061 ttttggaatt aaatccttga ttttatctaa tttttcatta gaaaaatcac ctctactaag
    24121 tatgtcattt aatttttggg tccattctct gtccttttct tcaattatag ttcgtttata
    24181 ctcttttgat gttttaataa tcttagattt ttcttctaat ttttctttca tatcaacacc
    24241 tcttttttat tattacccat ttcttcatac caaagtctga acttctaagt aaaatttgca
    24301 ttattgaaat aagaacctta taaacttata tatcattact aaagttaaga ctttaaagag
    24361 aagcacttag attacaatag attttagata gctttctttt taaaactatt gttgataaat
    24421 gaatgaatat aagggtatgt attaaaatag agatactcat ctttttcatt gtttaagtaa
    24481 tgaaaatata ttttatttaa tacaaaaaag agtgccttat aaactaaaag ttcacttcga
    24541 gacactccct tacattttgt ttcttaaact ttacaatatt acatacttat ttattaattt
    24601 aatctatgca tacttaaccc aataataaag tacatatatc caactcatta agccatgaaa
    24661 aatagcccat ggaatagatt tccatgcagt ataagaaata atcattgcta aacaagaacc
    24721 aaaagaaata ccctttttaa cagttgattt tatagtatta ttatttgaat ccataattaa
    24781 tcctccaaaa ttcaaaataa acaagttatt atatatctta tttgaatcta atacaatata
    24841 acagcttcta aataaattat actcttatta tacataattt ataaattata attccgataa
    24901 ttatatgaat taattaatta tattaaccta aaaatattat cctatctaaa agttatatga
    24961 tagatacaac atgtcaacac attgtaattc tccttcaaag attggttctg gataattatc
    25021 tacaaaaaag tttttaattt tatgtgaata aacaaatcca aacttctcat aaaaaggaac
    25081 tccactttca gatgtaccaa caaccattga tgaatatttt ttactataaa catctaaaag
    25141 atgataaatc atctttgagg cataacccat attttgaaaa tgctcatatg tagcaatgtt
    25201 ttttaattca catatttctt tggaaatttc atttacaact gctatacaag taagttcttt
    25261 attatcatac aatgcgtaca tagttccttt attgagatat ttatcaatca tttcttcaca
    25321 tgggtcagct aatattaaaa ggtcaatttg acttttttta tttatttcta tagtttttat
    25381 ttctatcata agttctccac cctcacttta ttcaaacctt aatttaacaa acttcttaca
    25441 gtacttattt agttcatttt gatatatatt tttacttttt aaaatatagt gataattccc
    25501 tagaatatta atattaataa taaattataa tatcagctct agcaattacc tagaatatca
    25561 atactaatat tcatctataa tattag
//
LOCUS       DAMJWS010000060        25544 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMJWS010000060 DAMJWS010000000
VERSION     DAMJWS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.060"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(262..978)
                     /gene="dapD"
                     /locus_tag="PC399_002486"
     CDS             complement(262..978)
                     /gene="dapD"
                     /locus_tag="PC399_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF2593340.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1202..1951)
                     /locus_tag="PC399_002487"
     CDS             complement(1202..1951)
                     /locus_tag="PC399_002487"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2593341.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(2018..2905)
                     /locus_tag="PC399_002488"
     CDS             complement(2018..2905)
                     /locus_tag="PC399_002488"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2593342.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(3022..4023)
                     /locus_tag="PC399_002489"
     CDS             complement(3022..4023)
                     /locus_tag="PC399_002489"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF2593343.1"
                     /translation="MKKVNVAIIGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4448..5329)
                     /locus_tag="PC399_002490"
     CDS             complement(4448..5329)
                     /locus_tag="PC399_002490"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2593344.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGIISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            5652..6845
                     /locus_tag="PC399_002491"
     CDS             5652..6845
                     /locus_tag="PC399_002491"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF2593345.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            7051..8169
                     /locus_tag="PC399_002492"
     CDS             7051..8169
                     /locus_tag="PC399_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF2593346.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            8300..8545
                     /locus_tag="PC399_002493"
     CDS             8300..8545
                     /locus_tag="PC399_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF2593347.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            8532..9278
                     /locus_tag="PC399_002494"
     CDS             8532..9278
                     /locus_tag="PC399_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2593348.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKSEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            9563..10438
                     /gene="hslO"
                     /locus_tag="PC399_002495"
     CDS             9563..10438
                     /gene="hslO"
                     /locus_tag="PC399_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF2593349.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            10829..11629
                     /locus_tag="PC399_002496"
     CDS             10829..11629
                     /locus_tag="PC399_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2593350.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            12156..14396
                     /locus_tag="PC399_002497"
     CDS             12156..14396
                     /locus_tag="PC399_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2593351.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            14810..16345
                     /locus_tag="PC399_002498"
     CDS             14810..16345
                     /locus_tag="PC399_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2593352.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            16361..17122
                     /locus_tag="PC399_002499"
     CDS             16361..17122
                     /locus_tag="PC399_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593353.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            17460..18329
                     /locus_tag="PC399_002500"
     CDS             17460..18329
                     /locus_tag="PC399_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2593354.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            18658..18909
                     /locus_tag="PC399_002501"
     CDS             18658..18909
                     /locus_tag="PC399_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF2593355.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            19021..19800
                     /locus_tag="PC399_002502"
     CDS             19021..19800
                     /locus_tag="PC399_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2593356.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(19935..20585)
                     /locus_tag="PC399_002503"
     CDS             complement(19935..20585)
                     /locus_tag="PC399_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593357.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(20582..21085)
                     /locus_tag="PC399_002504"
     CDS             complement(20582..21085)
                     /locus_tag="PC399_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2593358.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(21134..21649)
                     /locus_tag="PC399_002505"
     CDS             complement(21134..21649)
                     /locus_tag="PC399_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2593359.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            21929..22453
                     /locus_tag="PC399_002506"
     CDS             21929..22453
                     /locus_tag="PC399_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2593360.1"
                     /translation="MHEQIDCCERKKRTIDIKGENMRELLKWVSIINRYNTNYIDRKL
                     ADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVK
                     DKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMN
                     EEAKLEAKENGRKI"
     gene            22437..23840
                     /locus_tag="PC399_002507"
     CDS             22437..23840
                     /locus_tag="PC399_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2593361.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(24404..24715)
                     /locus_tag="PC399_002508"
     CDS             complement(24404..24715)
                     /locus_tag="PC399_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593362.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(24771..25364)
                     /locus_tag="PC399_002509"
     CDS             complement(24771..25364)
                     /locus_tag="PC399_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2593363.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         9488 a         3146 c         4216 g         8694 t
ORIGIN      
        1 ttaaattata aaaagtttaa tagtttatta cattgatata ttgaaaaatt gaaggaaata
       61 aaaatacttg tcagcaagaa ataaaaatga tataagaatg aataagagaa ttgtaaaaaa
      121 ataaaatgat tataaataaa agataaaaat attgtaaaaa agagggtgcc tctggtgaat
      181 taaattcaca cagagacacc tttttaatta attttttaaa ttagtatatt attgtttaat
      241 taatagcata gtactattta attaatctaa atttctaagg tcttccataa gcttagtttt
      301 gtcagccgtt ttttcatctt tcatttttat aactttagca ggagaaccag caacaacggc
      361 accagcttct acatcagtag ttacaacagc accagctgca acaacagcac ctttaccgat
      421 tctaacacct tcaagaataa ctgcattagc tcctattaaa acatcatctt caactataac
      481 aggagtagca gaaggtggct ctaaaacacc agcaacaaca gcaccagcac ctaagtgaac
      541 atttttacca agagttcctc tagctccaat aacagcattc atatctacca tagaaccttc
      601 accaataaca gctcctatat ttataacagc tcccatcatt acaacagcat ttttttctat
      661 agtaaccatg tctcttatta ttgcacctgg ctcaattctt gcatgttcac ttaaagtatt
      721 ttttagagga atagcagaat ttcttctatc atattctaca tgagtatctg ttatatcatt
      781 tccatgttta tctaatactt ccattatatc ctcatagtca cctacaaata tataagaacc
      841 attacttcca aataccttaa atttatcagt tgattggaag tgtaaagagt tagtatttac
      901 atatacctta acaggtgttt tctttttaac ttcttttata taccttgcta tttcataagc
      961 atcgtttaaa tttaccattt gttaattctc cttgtatttc attatatttc ttttattata
     1021 tacttatttg tcaattttag tatatactta cttcttatta tttatatttt attttagtta
     1081 gcttttattg tatagacata gtattaaata aatttattat atatgtttga tttgtattgc
     1141 atattattat atatatattg tatatgttta atttatattg tgtattatta tatttatact
     1201 attatcccaa catgctgtcc atattataaa atcctggttt ttgcttaact aagtatttgg
     1261 cagcagctat agaaccactt gcaaagatac cacgagattg agcttgatgg cttattgtaa
     1321 gaacctcatt gtctccagca aatagagcat catgttcgcc aacaatattt ccacctctta
     1381 tagcatgaat accaacttca tttggttgtc tttttgcact acgaccatat ctaccataat
     1441 tattttcaac gtttggcaaa acctctttta tagcgttagc aatcataaca gctgtgccac
     1501 ttggggcatc tacttttctg ttgtgatgtt tttctattat ttctatatca aatccttcta
     1561 gtaatttagc tgcatcttta accaatttta ctaatacatt tacaccaaga gacatattat
     1621 atgattggaa tacaggaata actttagccg cttcacgtat tttgttcatt tcgtcatcat
     1681 tatatccagt agtagcaatt actaaaggtg ttttagtctt taaaacgtaa gataagatat
     1741 tatctaaatt agagtgatga gaaaaatcta taactacatc agcttcctca actatagtac
     1801 tcatatcttc ataaatttta aggtcgcctt catagctatc cagacctcta gctgcaacac
     1861 aaaccaattc taattcatta tcttctctta tagtatcggc taaaatctta cccatttttc
     1921 catcataacc agttgctata acttttaaca taatttcctc ctatgattaa gggctaactt
     1981 ttgaccaaac ttacttttta ccatgcatta atctttacta tgcattaact tttagtccaa
     2041 agttaactaa ttctttcttt aatacttcta aatttgctgg gtccatttct gctagaggta
     2101 atcttaaatc tccaacattg aatcccaata aattcattgc agtcttaact ggtataggat
     2161 ttacttctat aaataaagct gcgattagag catccatacc aagttgtaat tttctagaac
     2221 cttcaatatc gccttcaaag aatttagcta ccaaatcgtg agtttcttga gggcaaacat
     2281 tagcaagtac tgatataact ccttgacctc ctaaagataa taaaggaagt atagtatcat
     2341 cattaccaga gtagatagca aagtcatctg gaactaaact agctatttct gcaacctgtc
     2401 ctaggtctcc actagcttct tttacagcaa ctatgttatc aattttagca agttcaacta
     2461 taaggctagg acttatattt acacaagttc ttccagggac attgtaaagt ataattggaa
     2521 gtttaacact gttagctatt gtttcaaaat gacgcttaag tccagcttta ttagctttat
     2581 tgtaataagg tgttattatt aatagagcat ctactcctaa tttttcagct tcttgactta
     2641 agttaactga gtgcatagta tcattagaac cacttccagc tataacagga actcttttat
     2701 taactttttc aacaacatac ttaatagtag caagttgttc ttcatcagtc atagtattag
     2761 cttcaccagt agtaccacaa gatactatag catcagtacc attagctatt tggtattcaa
     2821 ctaattcacc taatttgtca aaatcaactt tgttatcctt tgtaaatggt gtaactagag
     2881 caacagcaga acctttaaat atcatataaa atcaatcccc tcttttttat ttttgtgttt
     2941 tatattacag ttacttctaa tgcttgtttt tgagttacta ttaatatttt acaaaattat
     3001 atatttatac aagtttgtat attatactaa atcgtatgat aaagccattt cagctatttg
     3061 aattgcgtta gttgcagcac cttttcttat attatcagca acaacccaaa gatttacacc
     3121 attatcaaca ctaaaatctc ttcttattct tccaacaaat acttcatctc tatcatcaaa
     3181 ttctacagca gttggatatt cgtttttagc aggattatca actactacaa cacctggagc
     3241 ttcttcaagt gtttttacta aatcatctaa ctcaaatgga ttgttaaact caacatttat
     3301 agattcacta tgaccatttt ttactggaac tcttacagta gttgcagtta tttttaaatc
     3361 gtagttatca agtattttca tagtttcatt taccattttc atttcttctt tagtgtatcc
     3421 attttcagtg aatgaatcta tatgaggaag acagttgtaa gagatttgat gagggtagaa
     3481 tccagtagga ttaccgttca ttcctttctc taaatcttca acacctttaa caccagaacc
     3541 agatacagct tggtaagtag agtaaacaac tctcttaatt ttgtatttat catcaagagc
     3601 tttaagggga accatagctt gtatagtaga gcagttagga ttagctatta tacccttatg
     3661 attttttatg gcttctggat taacctcagg aactactaaa ggcacatctg gattcattct
     3721 ccattgacta gagttatcta ctactaagat tccatttgaa gcagctattg gagcatactt
     3781 ttcactaata cttccaccag cagagaataa tgctatttgt atgcctctat cagtaaatga
     3841 agtttccttt aattcttcta ctatatattc tttaccacaa aacattactt tagaaccagc
     3901 tgatttagaa gaagagaata aaaatagatt ttctattgga aaatttcttt cttctaatac
     3961 ttttaaaaat gttctaccta ccattccagt agcaccaatt attgctacat ttactttttt
     4021 catataaatt caccccaatt ttaaattatt aaaacattat tttattttag ttatacaagt
     4081 aagatatgtt aatattttta tatatccaaa aactttaata ttatattaac aactttaatt
     4141 atatacaaaa aacgtacaaa ggtatacttt gtacgtctaa tttatcactc aaagtaaacc
     4201 ctgtagctca tcacctacaa aaataagtga cagtattaca catgttatgt gcaatcccag
     4261 aaaatattat tgcaagaaat attttcttcg gctgtaaatc ctttttccaa tcattaacac
     4321 ctgccagcta ctattggata ctcttagtta cagctacctc taccctaggt taataggata
     4381 ttaaaatttg attattctaa taataataca tatttaagaa aaaatcaact tatttatata
     4441 aatattgcta aaatttaaat ccaaaatttg tcaattcttg ttttaacaca ttaagattag
     4501 tttcttccat ttcagctaga ggaagtctta aatcaccaac attaaaacct aaaatgttca
     4561 tggcagtttt aacaggaact ggatttactt ctataaataa agcagcaatt aaagcatcca
     4621 tatcaagttg taattttttt gagccttcaa tatcaccttc aaagaattta gttactaaat
     4681 catgtgtttc tttaggacat atgttagcaa gtacagagat aataccactt ccacctaatg
     4741 ataatagtgg tagtatagta tcatcattac cggagtatat agcaaagtct ttaggtacta
     4801 atttagctat ttctgcaact tgagctaagt ctccacttgc ttccttcaca gcaactatat
     4861 tatctatttt agcaagttca gccacaacag atggctttat attcacttta gttcttcctg
     4921 gtacgttgta taaaataatt ggcaacttaa cgctatttgc tattgtttca aagtgaagtt
     4981 taagaccttt ctcattagtc ttattatagt aaggagttat tattaaaaga ccatccaccc
     5041 ctaatttttc agcttcttga ctcatatgaa cagacaacat agtattgttt gaaccagtac
     5101 ctgctataac aggtattctc ttattaactt tttcaactgt atattttatt acagcaaaaa
     5161 tttcagattc actcatagta gtcgcctcac ctgttgttcc acaaacaact agtgcatctg
     5221 tgccattctc aatgtgatat tcgattaatt cacctaattt ttcaaagttg acattattat
     5281 cctctgtaaa tggggtaact aaagcaacag cagaaccttt aaataacata taatcaaatc
     5341 tcctttttta tctaaattta attttttaag tacaataata attatagtac aaattaatta
     5401 attttaatag gtatagttaa tatcttaact gtttaaataa gatatttata ttatattaat
     5461 attagaaaaa ttttaaataa atgttttatt ctcaaaatat gcatattaat ttaataaaat
     5521 ataccaaaat ggaaataaaa aataaatttt atgagaattt aataactaat aattaatata
     5581 tttaggttat aataaatatg tgcgtaaaat ctaacaataa gcaataatac cgtaaattta
     5641 ggaggaataa tatggatact ttaaaggaac tttttaaaat aggaagtgga ccttcaagtt
     5701 ctcatactat gggaccacaa agagcagcag agagatttaa gaatgaaaac ccagatgcag
     5761 aaagttttag agcaatatta tatggtagtt tggcagctac agggaaaggt cacttaactg
     5821 actatataat tgaaaaaact atagcaccta aaaaagtcga aattgtttgg gaagaagata
     5881 ttataaaaga ttttcaccca aatgggatga agtttgaagc tttagataaa gataaaaatg
     5941 taacagctga atggactgta tattctgtag gtggaggaac tatagcagaa gaaggtcaaa
     6001 gaaacagtaa aagcaatagt atatatcact tagatactat ggatgagata gtaaaatggt
     6061 gcaaagaaaa taataagact ttggtagatt ttgtgttgga atgtgaacca aaagacataa
     6121 aagattatat aaaaactata aaagatgcta tgagaaaatc tattgatgat ggactaagta
     6181 cagatgaaat aataccaggc aagttattat taaaaagaag agcaagtaca ttttataatg
     6241 catataaaaa agataagagc ttctcaacac ttgtatatgc atatgcacta gcagcttcag
     6301 agcaaaacgc ttctggtaat ataatagtta cagcaccaac ttgtggttct gctggagtaa
     6361 taccaggaat attctttgca atgcaggatt tctataatta tgatgacgaa aaaataattg
     6421 aagcactact tgtagctgga ataataggta atataataaa aactaacgct tcaatatctg
     6481 gagcagaggt agggtgtcaa ggtgaagttg gagccgcttg ttctatggca gcagcagcag
     6541 tagcatactt aaaaggtgga actatagacc atattgaata tgcggcagaa atagcacttg
     6601 aacatcattt aggaatgact tgtgaccctg tatatggata tgttcaaatt ccatgtatag
     6661 aaagaaatgc aatggcagct caaagagctt atgatgcagc taattatgca ttattaactg
     6721 atggctcaca ttctgtctcc ttagaccaag ttgttgaaac aatgaaagaa actggtatag
     6781 atatgatgga taagtacaaa gaaacagcta agggtggact tgctaaacat ttcttctctt
     6841 gctaaaaata aaattttcca ataataattc ctctgttttg atttatgaaa caggggaatt
     6901 attattaatg gtgaattatt taataatgca aaatattttt atattcaatt tagcttttta
     6961 tttttaatta gtataacatg ctatataata ttttaattat aaagaaatat agaaatattt
     7021 tttaataaat tagaaattgg aggagattac atgagagagg catcatattt acaagaacaa
     7081 ctttataaac ataggaagtc attaaatcct attgcagaga ttgggagaaa agagtataag
     7141 acttcaaaat atataagaga ttatcttgat aaattaggaa ttgagtatga agtttattta
     7201 gaaacaggta tagtaggtgt aatagaaggt aaaagaccta aaaaagatat tgcttttaga
     7261 gctgacatag atggtcttca tagtgaagaa ggggtaaaac atttatgtgg tcatgatgga
     7321 catgctacta tcttacttgg attaattgag tatttaaatg ataataaaga agacttaaat
     7381 gataatatag tatttatatt ccaaccggca gaagaaggtc ctgggggagc aaaagcttta
     7441 atagatgaag gagtgcttag aaaatacaat attgatgaaa tatatgggct tcatatatat
     7501 ccagagttac cagaaggtta tgtaggtaca agagagggct attttatggc tcaaatagga
     7561 gatattgaca tagaaatagt gtctaaaagt ggacatgggg ctatgccaca aaatggtatt
     7621 gatggtatag tgatagcttc aaactttgta agctcacttc aaactatagt gtctagaaat
     7681 ataagcccaa tagacaatgc agtgttgact attggcagaa ttgaaggggg agctagaaga
     7741 aatataatag ctgaaaatat aaagatggaa ggtactatga gatgtttcag cccagaagtg
     7801 tatgagaaaa tgaagcttag agttagagaa cttgctagag ggtttgaatt ggcttataac
     7861 tgtaaggtta atataaatat tatagatgat tatatcgctg ttaaaaatga caaggatttg
     7921 tatcaagagt ttgtagaggc tattggaagt gatactgttg tagagttaga gccacttatg
     7981 atatcagaag atttttctta ttatcaaaaa gaagttccag gattattttt tatgttgggt
     8041 tcaagaaacg aagaaaaagg atttgtaaat ggtcttcata atataaactt taattttgat
     8101 gaaaagatat gtgtaaatgc attgaatgtt tattcaaaat tattaaaata caaagaagca
     8161 atagattagg aagataagtt ataaagatta aaaagcataa atacctaaat ttaaaaaaag
     8221 tgtattaata aatacccctt acatatttgt ataaatttat tttaattgac aaaaatacaa
     8281 ataagaaggg gtgatttttt tgaaagaaaa agtacaaaga gaaaagaaga taactaatgg
     8341 caagaatact aaaatattga caaatgatga tataatgaaa tatgagatag cctcagaact
     8401 aggtcttatg gataaagttg gtgaacgtgg atgggcagga cttacagcta aggaagcagg
     8461 aagaattgga ggtatgctta catcacgcaa aaagaggctt aaaaagattg tggaggagca
     8521 gaataaaaag gatgagtcaa tatagtgatt ttgcgtttgt atatgatgaa cttatgaatg
     8581 aagttgatta taatggttgg gttaaatata tagaagatat aatagatagt gaaggtgtaa
     8641 aagtaaaaaa tatattggaa ttggcatgtg gcacgggtaa tcttacaatt ccattgacta
     8701 aaaaaaatta cgatatagct ggtatagata tatctgatga gatgcttagt gtagcaagag
     8761 aaaagtcaga aaaagaagga gtagaattag ttttacttca acaagatata tcagagcttg
     8821 attttgaaat atctgatttg gactgtgtac tttgtgcttg tgatggattc aattatataa
     8881 cttatgatga tgatttggaa aatgtatttt caaagacata tgaattatta aagaaagatg
     8941 gaatattcat atttgatata agttcttttt ataaattagc caacatattg ggaaataaca
     9001 tgtatggaga aaatagagaa gatatagcgt atatgtggca aaattacttt gatgaagaag
     9061 agaatctaat tgaaatggaa cttacattct ttattaaaga tgaatgtgga aaatttgaaa
     9121 gatttgaaga gatacaccaa caaagagcat atactgaaga tgagatagtt gatatgcttg
     9181 aaaaatctgg atttaaggat ataaaatatt atggggattt tacatttgac aaacctagtg
     9241 aagaaagtca aagaatcttc tttgtatgta aaaaataaga tatttttgac tatataagat
     9301 aattattaaa aataaccatg atataaaaat atagaaaatg cagttttata gattaaaagt
     9361 aaagtgatga tactttatga cttttatttt ttaataatta taaagttcta tagataaaaa
     9421 ttaaaatata ataactattg cttagtaaaa ttaaaataaa atgggttttg cccaaggtta
     9481 agatagataa gtatttatag attaaaatta aggtagtaat ataataaaaa taagacaatt
     9541 aacgggacat ggaggaatat taatgagaga ttatgtacta agagcgacat caggaaatgg
     9601 tcaagttaga gcatttgtcg caactactag aaatacagta gaagaagcta gaagattaca
     9661 tgaaactaca aaagtagcaa cagcagcact tggaagaaca ttaacagcca catctataat
     9721 gggattaatg atgaaaaatg atagtgataa attgactgta ataataaaag gtggaggtcc
     9781 aattggaact attatcgcca catcagactc taagggaatg gtaaaaggat atgtaggaaa
     9841 tccacaagta gaggttgaag actatccaaa tggaaagtta aatgtagcag cagcagtagg
     9901 tacagaaggt gttgtaaaag ttataaaaga cttaggtctt agagaaccat ataatgggac
     9961 ttatccattg gtaagtggag aaattgcaga agactttact tattattttg ctgtatcaga
    10021 gcagactcca tcagtagtag ctttaggtgt tcttacaaaa gaagatgaag tagagtttgc
    10081 tggaggattt atagttcaat taatgccaga tgcagaagaa gagactattg ctaagttaga
    10141 ggaaaatgta gctaaattac cttctataac taacatgtta aaagaaggaa aatcaccaga
    10201 ggatatatta aatattgttt tggatggttt ggaacctaaa atacttgata cctgtgaagt
    10261 tgggtttatg tgtgaatgtt caaaagaaag agtaaaaact gctttggttg caattggtaa
    10321 aaaatcattg gctcaaataa tagaagaaga taaaaaagca gaagttggtt gccagttctg
    10381 taataagaaa tatatgtata gtgaagaaga gttgcttgaa attttaaagg aaatgtagac
    10441 tataaaaata gttgtattga tataagtaat gttatagtga tatagtaaag tcgtattgat
    10501 atagtaatta aagtataatt tattaataat agacatattt atattaaaaa ataagtttaa
    10561 taaattttta agcttaataa attttaagaa ttaagaagct ttctggctgt aatgtttgca
    10621 atgttgcagt tagaaggctt ttttatttta ttatagttcg tgtcaaagat tgtatgaaac
    10681 aataaaatat tgtaaaataa tttgtatatt tagacctgat tggcacaaag gtttataagg
    10741 tatttgagtt gattatgcga aattctatga agtagtaacc ctgtgacttt aatcatggga
    10801 ggttcagaag aaggagaaaa ttttaaatat ggaaagtaaa acgaagtaca taggaataag
    10861 tatattaata ttattgatga tatttttaat tgcaaaaaat tttaacggaa aacttgaaat
    10921 acctattcct gagagtaata gtagaaaatt taaatcagca gcagtgtttt atcctcaaca
    10981 tcaagatgat gaggttttat ggggaggaag tgccattgta gatgctataa aacagtgtgg
    11041 tgttgataat gtatatgtgg tattagtatc agatggttct ggagttaacg ttttcaaagc
    11101 taacacaaaa tttagaaatt taactaggaa gcaaaaggaa gaacttagaa ataacgagtt
    11161 taaatcagct ttgagagagc ttggagtgaa acctcaaaat gtgattatac ttgcagatat
    11221 agataaaaag gaaggcacac attatgaatt aatggaaaag actatattgg attttgaaca
    11281 taagtttaaa ggcaatgtga cccatatagc ccatcactat aagtatgatg accatataat
    11341 gcatagaaag aatggggaag ttttaaagaa acttaaacgt gaaggtaaga taaaagatga
    11401 cttgtatttt gttaagccaa aatatgtaaa gtatatacca agaaatgaaa gagctatcta
    11461 taaagcaatg gattttgatg actacaataa ggttaagagt gcttgttatg aatataaggt
    11521 tgtaaattca aagcgtaaaa tgtatggtat aggatatatt tcggcacata gttattttga
    11581 acatcttcta aatgaccctg aacttacgtc tgtattaagt gaagattaat ttcttataag
    11641 tagtaagtta tgtttgataa gttgattgga attttctata gttttgaagg tattggaaat
    11701 ttagttttat ttaaagattt aaagatagag gtttttagtg tttaaaattt taaagttaag
    11761 ttgaataatt gtatagaatt atttgtgaaa gaagaaacag tgtaaaacat agtatcttat
    11821 ttatggtttt aaaatcatag gcaagatact ttttttatgt aactaatacc attttgagat
    11881 tattaactta agaattttat ataatttaac actcatatat aatgatgata tttaggtaga
    11941 atagatgcaa ttacgcttaa ataaaataac tgtatttttt ataaaaatca aaccctacac
    12001 tatactacca gataaaatct acatgctata atttgacctg tgtttataaa aaaaggagta
    12061 atctaataaa atagtatttc acttatgtat aactaacaac tattattacg attttctcta
    12121 aataatttaa agaaaaataa tgcaggaggt aaaatatgaa caatgaattt ataattttaa
    12181 aaggttgcaa agagaacaat ttgaacaata tttccttaaa aataccaaaa agaaaaataa
    12241 ccatattcac aggggtgtcg ggttctggaa agtcatctat tgtatttgag acaattgcaa
    12301 aagaatcaca acgccaacta aatgaaaggt ttagtacatt tgtaagaagt tttttaccta
    12361 agtatggaga ggttaaagct gattgtatag aaaatttgtc aacaccaatt atcatagacc
    12421 aaagtagact tggtggaaat tcaaggtcaa ccctaggtac tattacagat gtaaattcct
    12481 ttttaagagc tttatattca aggtttggta gtgagtatat aggtaatgct aatatgtttt
    12541 cttttaataa tattgatggt atgtgcccac actgtcatgg gcttggaaaa aagctagttc
    12601 ctaatatgaa ccaaattctt gatatgaata aatcattaaa tgaaggtgct atattgcttt
    12661 cagggtttgg agtgggttct tggcattgga atatattcgc tgaatcagga ttttttgata
    12721 ttaataagag gatatgtgat tattcagaag tagaattgga aaagtttttg catggtaaag
    12781 cagaaaagat taagatagaa aatgctggtc aaactaacat gacatatgag ggattaatgg
    12841 ttaaatttaa tcgcctatac ttaagtagag agggagaaat atctgaagct actaagaaaa
    12901 aattgagtaa acttcttata gaagatagat gtcctatatg tgatggaaga agacttaatg
    12961 aaagagtata taggtcttta attaatggtt ataatatagc agatttaact agtatgcaaa
    13021 ttgatgaatt ggctgaagtt attaaaagta tagatgagcc agaagcacaa cctttaatta
    13081 aaggaataat agaaaagtta aacagtatca tagatatagg tcttggatat ttaacactgg
    13141 atagagagac atcttctcta tcaggagggg aatctcagcg tataaagatg gttaagtatt
    13201 taaatagtaa tcttgttgat ttaatgtata tttttgatga accaagtgta ggacttcatc
    13261 caaaagatgt gtataaatta aataatcttt taaaaaaatt gcgtgataaa gggaatacaa
    13321 ttatagttgt tgaacatgac ccagatgtta ttaagattgc agaccatatt gtagatgtag
    13381 gaccaaaggc aggtaagtat ggtggagaag ttgtgtatga aggcagttat gaaaatctac
    13441 ttacaagtgg tactttgaca gggaatgctt taagtaaatt tcttcctatt aaagaaagtg
    13501 taagagaaca tagtggatat ttagaagtta aaaattgtaa taaaaacaat ttaaaaaatg
    13561 tatcgattaa gatacctaaa ggtgtattga ctctagttac tggagtagca ggagcaggta
    13621 aaagtacgtt gattaaggat gaatttttaa agcaaaatcc tagtgctgta cttatagacc
    13681 aaagccctgt ttcagcaaat tcaaggtcga gtttggcaac atatagtgga attatgaaca
    13741 acataagaag catattttca aaaacaaatg gcgtaaatgc ttctttattc agttcaaatt
    13801 cagaaggagc ttgtgataac tgtaagggtt ctggaatagt agaaatgaat cttgctttta
    13861 tggagagtat aaagagtacc tgcaatgttt gtgaaggtaa aaaatttaaa aaagaagttt
    13921 tagaatatag gtttcaaggg aagaatataa ttgaagttct tgaaatgtct gttttagagg
    13981 caattgagtt ttttaattta aaacagatta aaacgaaatt acaatgtatt gaaaaaatgg
    14041 gtattggata ccttacttta ggacaaactc ttgatacttt gtctggagga gagtgtcaaa
    14101 gattaaagct tgctagtgaa ttaaataagg aaagttcagt gtatatatta gatgaaccaa
    14161 caacaggtct tcatatggct gatattgaga attttataag tataattgaa gagattgtag
    14221 ataatggaaa tacggtcata ataattgaac acaatataga tgtaattaaa agagctgatt
    14281 ggattattga gctgggacct gaaggaggaa caaaaggcgg gagagtaata tttgaaggta
    14341 ttccaaaaca attatgtaac aataaaatat cattaacagc aaaatatata gtataataaa
    14401 taaaatataa taaaattata atgatatttt aataaatata atgggtatat aaaatgataa
    14461 ttaaacttaa cagtaataaa taatgtgaga aatattcaaa tttaattaaa taaagtgtca
    14521 gaaatttgtt taaattttag tattttaacg gttctgacct tttttcttat ttttatggag
    14581 aatatcgagg atggagtctc taatagttac ttttttgtgt ctacttgcaa gtttgattaa
    14641 aaaatggcta tacacacttg tgacttcagt catgggaggt tcagattata atcttaatat
    14701 ataatttgtg aaataatttg atgacgattt gctgatgtat aaacaataaa gattaactac
    14761 attaaaattt aaatcttgtt aagtaaaact tataaaagga gagagatgga tgataaatga
    14821 tattctaacc ttattatctg aaagaagtga tttttttata aagctatcca tagaacattt
    14881 gatgatttct attatttcta taataattgc gattgttttg ggtggtataa ttggtattat
    14941 tgtaagtgaa tttcataatt cttctaaacc tatattggga actatcaatt ttctatacac
    15001 cattccatct atttctatgc taggatttct gataccattt tccggaattg gaaatactac
    15061 tgctgttatt gcacttacca tttatgcatt attgccaatg gttagaaata catatactgg
    15121 aattactaat gtggataaaa atattcttga agcagctagg ggaatgggaa gtacagattt
    15181 tcagatattg tataaaataa aacttccgtt agccctacct gttattatgt ctggtattag
    15241 aaatatggtg attatgacaa ttgcattagc tggtatcgct tcatttatag gtgctggagg
    15301 tctaggtgta gccatatata gaggaatcac gacaaataac acaataatga caattactgg
    15361 aagtatactt attgctctat tagctttaat atttgatttt attttagggc tggtagaaaa
    15421 attttttcaa cgaaaaagaa aaaagaaaaa taggaaaaaa gtttttgcca ttctaggaag
    15481 ccttattctt atagcaacta tctttacgtc ttatttattt aatccttcaa agactcaaac
    15541 tattcatatt gctactaaac ctatgactga acaatatatt cttggtgaga tgttggatat
    15601 tttaattgaa aaaaacacag gtctaaacgt ggaaattaca caaggtgtag gagggggaac
    15661 atctaatatc cagccagcta tggagaatgg agaatttgac atttatccag agtatacagg
    15721 tacaggctgg aatatggtac ttaagaaaaa tgaattatat actgaaagta tgtttagtaa
    15781 actacagtcg gaatacaaaa ctaaacttaa aatgcaatgg actggtatgt atggtttttc
    15841 taatagctat gcattagcag ttagaaaaga agttgcagat aaatacaatt taaaaaatat
    15901 aacggactta aaagatgtat cttctaaatt gacatttgga gctgaatatg acttttatga
    15961 gagagaggat ggatatactc cattgtgtaa tacatatgga ctgaacttta aagatacagt
    16021 tgatttagat attggattaa aataccaagc tatcaatgaa ggtaagattg acgtaatgcc
    16081 tatatttaca actgatggtc agtatagtgt ttcagatgtt gtagttctaa aagatgataa
    16141 aaatttctac ccatcttatc aatgtggaaa tgttgttcgt ttggaagtat tagaggaaaa
    16201 tccagaacta tcaaaggtat ttaagatggt tggagatatt ttgactgata aagaaatggc
    16261 taaaatgaat tatgacgtag aaaacaacaa tttagaacct agagaagttg ctcgtgagtt
    16321 tctgaataag aaaggtcttt tataaattgg aggaaatata atgacaccta ttatacaatt
    16381 taaaaatatt aaaaaacaat ataatgataa aactataata gataatctta atttagatat
    16441 agaaaaagga gagtttttga ctgtaattgg ttcgtctggt tctggtaaga caacactctt
    16501 aaaaatgatt aatggtctga ttttgccaga tggaggaaat attttgataa accaaacaga
    16561 tatcaaaaac gaggacttaa ttaaattaag aagaagaata ggatattgtg tacagggaag
    16621 tgttcttttt cctcatatga ctgttgaaga aaatatttca tatgtaccaa atctcctaag
    16681 taaaaagaat aaattagaag taaaaagtgc tgtaaacaag tggatggaaa tagtgggatt
    16741 gccaaatgat atgaaagtgc gttacccatc agaattgtca ggtggtcagc aacaacgtgt
    16801 agggattgca agagctttag cttcttctcc agaaatttta ttaatggatg aaccttttgg
    16861 tgcagttgat gagattacac gaaagcaatt acaaaaagag ataaaagaaa ttcataaaaa
    16921 aacaggagtt acaattatct ttattacgca tgatatttat gaagctttga ttttgggaac
    16981 aaagactttg gtattaaatc atggagttgt tcaacagtat gatactcctg agaatatttt
    17041 aaatactcct gctaaccaat ttgtagacca gttattgaat ataagaaaat ctataataga
    17101 tgatgataat ttaaaagaat aaatttaaat ttaaaatcaa attgttgtga taaaaactat
    17161 tgatttagag ctaacttcaa gttgtatact tgaagtggaa aagttagatt aaggtataga
    17221 agggcattta gcagttggct gtagacgagg taactgctga aggtgctttc aataaatggc
    17281 tagaacctgt atctgttgaa gattataata aattgaaata agaagctcaa tatacagagt
    17341 agataggcac ttcaaataga agtgtctttt gtgtgccata aaataaatca aaatattagc
    17401 aaagaatgtg aaaaacaaaa taaaataata tgctatcttt aaaataggag ggatgctata
    17461 tggataacaa tttgattacc attgaaaaaa ctttagatta tatagaaaaa catcttttag
    17521 atgagatgtt agatttaaat gtaatttcta aagagatagg ctattcaaaa taccatttac
    17581 atagaatgtt tacatcagtt gtcggattta gtgtacataa ttatatacaa agacgtagac
    17641 ttactgaagc agcgagaatg ctaattttta cagaaaaacc aattattgag attgctttaa
    17701 tctcggggta tgatacacaa cgctcttttt caagagggtt taaaaaaatg tttaaacgta
    17761 gtcctaattt ttttagaaga catcaagatt ttttaccact acaattaaaa tttgatattc
    17821 ataatagaaa aaaattacgt ggagatatgg ttttagatgt taaaaaagtt aattgtgcag
    17881 atataaatat tgttggctat tctggtaaag taagaaaagg atttcatatt attggaaaat
    17941 gctggaggga tttgcataaa aataaagaga tgatagaaaa taaggtagat ttaggttttt
    18001 taattggagt tagtgattat agcaatttta aagagattga gaatactcct acatttacat
    18061 acattgcagg tgctcaagta gctacttttg gcaaattacc taatggaatg gaaaaattcg
    18121 tattaccgaa aagtaattat attgttttta cttttattgg gaaaaatgag gatagtatgc
    18181 aaaatgttgt agaatatatc tatcaagaat ggttccctaa ttcaagttgc cagtttaatg
    18241 aaaataattt atatgacttt gtaaaatatg gagaaaaaac tgatgagaat ggtttgagtg
    18301 aaattcagtt ttgggttcca atcatttaaa agaaggtatt atgtataaag tagatggatt
    18361 tatataggca aagttttaca gcagtatgta tttataaata atttatagtt tgtaggaaag
    18421 aaataatttt actataagat tatgataaaa tacacaatac tatgacaaaa tagtgttaaa
    18481 taatagattt aattttgcat aggaaaagtt ctataatggt gtgtattcat aaataattta
    18541 taatctgtag gaaagaaata gttttactat aagattatga taaaatacac aatactatga
    18601 taaaatagta ctaaataata gatttaattt tgcaaaggat aaggtgtttt aataaaaatg
    18661 aatgtagaaa acgaattact taaaaaccta gatagattgc atacaacaga gttaggcgtt
    18721 gtaagaatta agaaaaatct ttctttggag acaaatgatg tagtgaactg gtgtaaaact
    18781 aaaatagaat ctccaaatgc cattattaat agaaagggaa aaaactggta tataagtgta
    18841 tatgattgta ttataacagt aaacgctcac agttatacaa ttattacagc tcataaagaa
    18901 aagaaatgaa ttgcaaaata caaaggtcaa aagcattttc cactgtttgt agtacctatg
    18961 aagaagtata taaaataata ttacttttga ataataattt aaattttgga ggttgaaaaa
    19021 atgaatgata ctgtaaaaga aaaattggca gagtcactaa cttctgatac tactgatatt
    19081 ttaccttatt tgccttattt attacaagat ttttgggaat taggaagttc accagaacaa
    19141 atgttacaat tatttagaaa gcatatacct tttaatgagg atacaaaagt tttggattta
    19201 gcttgtggaa aaggtgctgt atctgtgaag cttgcaaaag agttgaatat acatatcaaa
    19261 ggtattgatt tgatacctga atttattgat tatgctagaa gaaaggctat tgaatataag
    19321 gttgatgctt tgtgtgattt tgaaatgaat gatgctaata gttctgtaaa aattgagcat
    19381 gattatgatt gtgtgatttt tggagctgta ggggatattt taggtaatta taaagaaact
    19441 ataagtaagc tattacctac aataaaaagt aaggggttca ttttaattga tgatgcatat
    19501 gtagtagata aatctacaaa tgaacagctt aattttgaga gggtgtatcc cacttacaat
    19561 gagtggtgta tattgttcaa agaattaggt ctgaagctaa ttgaatgtat accatgttta
    19621 gaacttgaaa atgatgttga agaggaaatg aattggatta taactcgtgc aaatgagcta
    19681 attaaaaaat atccttctca aaaagatatg tttgaacaat atgttattag ccaaaaaaat
    19741 gaatatactg atttacagca ggaccttgtt ggagcaactt ggattttaca aaaagaataa
    19801 gataaatatt agactagata atcaatgtta tagtgaatta tcccatgtct agtgaatata
    19861 aataacataa aaaaccgtca agatgaatag tttattcgtg cttgacggtt tttagtattt
    19921 gttgaagtaa aattttattt ctttagtata tcacttttag tcaatccatt tgatttagct
    19981 tgagtattcc attgatgatt ccccataagt ttatatagga atctagggaa gttaggtgaa
    20041 ataaaaatag tacttttgtt ggaatcatct atatgtatat tatcactaag tatatttttt
    20101 gaaagttctt ttaatgcaat tcctaaatct tttttaggtc ctttacccaa aggtacggat
    20161 tctagtgaac caaacatctc cccagctcca attccaatac cttgaaccca tgtaaagttg
    20221 atttttttac accaatgctc tatcatttga attgccaaat gattatgttc accttcattg
    20281 aatccacagt taactattgc atacactgga ataggttttt tattttgtgt ttttaaaaat
    20341 ttttcacatt tctctaaaaa atgaagtaaa tgagatggaa taccatctac ataaagagga
    20401 gatgcgatta ttaatctatc acaattaagt atggcattaa gagcgtcagg atacttagat
    20461 gtgttatcta ctttaaatgt aaaaacttta ttatcatttt gaataagttg ttttaattca
    20521 gataaaaaat actgtgaatt atttttattt acttttggac taccatttat cattactata
    20581 ttcatatgga ttcacctctt atatcttgta tattttcata gaaacaaaca gagtgttctt
    20641 ttatattgaa atttatagca tttccattta ctaatttcgt agcagtttct ttttcagctt
    20701 ctgtaataca atctccatag aatatagctt ttaaatttat ctgtttatca tatctgcttt
    20761 tatgacgcat catattatct tttattacaa agtatggaag aatataatta atgcttttat
    20821 caagtacgtt tttgacaaaa gggctaaacc ctccgtaaca gcatctgctt attataataa
    20881 ttccatcaga gtcatttata agtttaccca tgttgctgta ataatctttt acaacacatt
    20941 cagaaggagt tttaatccag caaccaaaac aaccaataca tttgtggatt tcttttgtgt
    21001 ttggaagtat ttttatattg tcatcaagtt tgggtaataa cttttctaat tctttgtcag
    21061 ttaaatcatg tattaaaagc ctcataaagt ccatctcctt ttattatgta tgtttacttc
    21121 taaaaattat atcttataga tttttcagta aatctttaca ccaattaata acaaaattat
    21181 aatagtcaac tccataaatc attgttgcat acatatatgt atattggtca acacaatgat
    21241 tttcttctgc atcatttttt tgtttactta atttatttag aagtaattct acttcagtta
    21301 ttaacatttt taagtttgtt tcaattattt ctttgggtag atacttgtaa aagaatatat
    21361 taacaaggtg gtcttgcttt gttttagaaa aatttatagg tttctttaac cattcaagaa
    21421 atacttcttt accagtatta gttatggaat atagtttttt taatttgtta ccatctataa
    21481 cttcgtgata gtgtatatat ccttttgttt ctaatctttt aagtgcagga tagatacttc
    21541 caaagctggc gtcaaaaaag tacgaggtac tttctgacat tatttgtttt aagtcgtatc
    21601 cacttaattc tttttccatt aaaaatccta aaataatgta ttctaacata ttacctcctg
    21661 gtatcaatta gatatatatc gattcgatat atttattatt acagataata aatcttacgt
    21721 caagatgctt tttaagataa gctaaaaaga attaggagtt attgataaat atgcaaaaaa
    21781 attaatataa aaggtaatta aatatcaata aagatttaaa aatagttaaa tcaaagtaag
    21841 agtctaatat ataaaattta tagtaacttt atggtttaaa tataaacttg tgtgctattg
    21901 acaaagtata aaattagcag tagaataatt gcatgagcaa atagattgtt gcgaacgcaa
    21961 aaaaaggaca atagatatta aaggagagaa tatgagagag ctattaaaat gggtttccat
    22021 tataaataga tacaatacaa attatattga caggaaattg gcggatattg gaatcaataa
    22081 tagccaatat ttttatgtat tacacatatg taataatcca ggtattacac aggacactat
    22141 atttgagaaa attcttgtga atcctagtaa tataacaaga gctttggcaa atttagaaaa
    22201 agaaggcttt ataacaagag aaccaggtgt aaaggataaa agaacatggc atttatatcc
    22261 tacagaaaaa tctttaggat gttatgatga aatactaaaa ataacaaatg ggtatgttga
    22321 ggagttgttg agttcatttg agaaagaaga gcaagagttg tttatgtcaa tgttaaagaa
    22381 agttgcattt ttggctattg atatgaatga agaagcaaaa ttggaggcga aggaaaatgg
    22441 aagaaaaata taaggagcca ttggaatatg aaaaggtttc aaagttatta actaagtacg
    22501 caattcctag tattatagca atgttagtaa gtgctttata taacattgtt gaccaaatat
    22561 ttattgggca aggtgttggt gtattgggca atgctgctac aaatgtcact tttccactaa
    22621 caacaatatg tactgcaatt gcattattgt taggaattgg aggagcgtct aagtgttcat
    22681 tggaattagg tgctaaaaat tcagaaaaag ctacaaaagc agcaggaaat tctatatgct
    22741 tgatggctgt ttttggaata agtttattta tagtagtatc aatattttta acaccactat
    22801 taaaattctt tggctctaca tcagaaatac taccttatgc acacacatat acaaaaatta
    22861 catcaattgg attaccattt cttataatgt ccactggaat gagtaagttg atacttgcag
    22921 atgggagacc gaaagcttct atgttttgta tgttaatagg agcagttatt aatacaattt
    22981 tgaatccatt attcattttt gtttttaata tgggaattgc tgggtctgct ttagcaactg
    23041 ttattgggca acttatatct tttggaataa gtgttcatta tataaagaat tttcaacata
    23101 ttaaattaca taagaaaagt tttaaattag atagtataat ttataaacat atatttgcgc
    23161 taggttcaag ctcatgtttt aatcaattag caatgactgt aacacagata gttatgaata
    23221 atacattggc atattatggg gctaggtcag tttatggaag tgagattcca cttgcttgtg
    23281 tcggtatcat tattaaagta aatatgattt ttatatctat aattattggt atatcacaag
    23341 gtatgcaacc aattataggg tataattatg gggctgaaaa gtataatcgt gtaaaagaag
    23401 catataagtt tgcagtggga agtgcaacta ttatatctgt tattgcattc ttatgtttcc
    23461 aattattccc aagacaaatt acgtctatat ttggtaacgg aaatgagact tattttatat
    23521 ttgcagaaca ttattttaga actttcttgt tcttaacatt tataaatggt gttcaaatag
    23581 tatcatcaac tttctttaca tcaattggga aatcagtgtt gggacttttt acttctttga
    23641 ctcgtcaaat tttattttta attccattga tagtaatttt accaatgttt atgggaattg
    23701 atggagtaat gtttgcagga ccaatagcag atgcagcagc ggcaatggta tgtatatact
    23761 ttgcagttca tgaaatgaaa tcattgacta taaagcaaaa taatttgatg agtaaccata
    23821 ataagattgc agaggtataa gacaatattt aagaagtata gaaaattaat ttaaagaagt
    23881 ataataaact aatttaggaa gtatagaaaa ttaatctaaa gaagtataat aaactaattt
    23941 agaaagtata gaaaattaat ctaaagaagt gtaataaact aatttaggaa gtatagaaaa
    24001 ttaatctaaa gaagtgtaat aaactaattt aggaagtata gaaaattaat ttaaagaagt
    24061 ataataaact aatttaggaa gtatagaaaa ttaatttaaa gaagtataat aaactaattc
    24121 aggaagtata gaaagctaat ctaaagaagt atagtaaact aatttaggaa gtatagaaaa
    24181 ttaatctaaa gaagtataat aaactaattt aagaaatata gaaaattaat ttaaagaaat
    24241 acaatacatt aatttttaag taacattaaa ttaatgtaag taaaataatg ttcatatgaa
    24301 gatttaaagg tgcattttct gaagctaagt atatgttgta gagtgttagg tctctctatc
    24361 ctgtgaagtt taatagctca agaaaatgca ctatttatga tgattatttt gttttatttc
    24421 caattttgga aataactatt ctaattaaat ctaaaattat accataaata agaaaagaaa
    24481 gtatagatgc tatgtaccaa taaatcgaaa ttgaccctgt aattgttcta catgggattt
    24541 gcatacctga atccataaaa acaaaaaaac tatcaactct ggaaataata gacaaaatag
    24601 gatttaaacc tattagaaca atactttttg agtcttgacc aatttgatgc atataaatct
    24661 caaataatgc aattaaaaaa aaccaaaatg taaaagattt aataattttt ttcatcaaaa
    24721 tacctcacat aatataaatt ctgtttgcac tatttgctta ctacaactat ttatctccat
    24781 tcagtcacat cacttaattg taatctaggt cttcttgatg gttcttcatc aggataaccc
    24841 aaagatattg ctgccactaa ttgactatct gtatttaacc attcacataa ttcacgatat
    24901 gcaaagtata catcacaaat ccaaagacta cctagaccta attccacagc tgtaagcgac
    24961 atattctgaa ttgcagctcc aattgattgc atgtttgcca tttcatagaa tcgttcttca
    25021 ggggatagct tttctaaagg tgatttacct aatatattta aaataaaaat tgttactgga
    25081 gcttgtttca tgatttcaac tgtgtaattt gcaccagcga tatgttgacg acttcctgga
    25141 agaagcccat tatcattaat ttcattttga atacctttgc tcatggcttt aagcatagat
    25201 tctttttcct tttcagtgat gacaacaaaa cgccatggtt gcctgttttt agatgaaggt
    25261 gcatttattc cagcttcaat tattttctct atagtttcat gtgaaatgct ttgattttta
    25321 tattttctga tacttcgtct ttttgatata gaatcactta tcattttaag taacctccgt
    25381 ttattttttg gaaaaatcta tttaattttt attttctatt ttaattattt tagcattaat
    25441 cagaaaaaaa gtcattgatt ttagtataca attattaatg attatattaa gaattgtatc
    25501 aaaaagtatg tgattatact aagggattat attaaacatt atat
//
LOCUS       DAMJWS010000061        25123 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMJWS010000061 DAMJWS010000000
VERSION     DAMJWS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25123
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.061"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            174..2606
                     /locus_tag="PC399_002510"
     CDS             174..2606
                     /locus_tag="PC399_002510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593364.1"
                     /translation="MNIAINSYLNNTLNPNNHILTKLDKGMPLLYKGQIVIFKNINNT
                     HITLGHKNKTDTTLIPIDSAYMLITYNGHLEVNSMGKTPASKTFQTKNVLSSLGFSDL
                     KNMTGVINDSTLIILPCKDDISDLVSNIKIKDINNTYKFTELFPCSYISSTGAETDYP
                     GNHAKQIPLLKFTTNISSAFEIIKSDKSIKNVFIIGDYPIYKHINDFERILNRKRIES
                     VNIVTSNSNISNILDLPNIDNLNIYSWSKDVLLTYSQDFENTSTFQKLWLDKLINKNI
                     CTTSVDSNISDLIHQARKSLYYISKYDFDISIKNSLMMCSYSLLKILENTPFNLDFLE
                     ETIKLLNLNIATPSAKLENIKYLISSTSPDLELSALFDDVIVKFERIINELSDNNTKF
                     NTLNKLVSSYKYKTFTPNTPVILLQKQYEAIILKNLLKSRNINLQVISAEKFKDSKTI
                     KNLIMFGFYDLKYLDILNSDLIINITLYLYNSELSKYNYYMNQINSARNKIEKNNKIY
                     NLLDIKPFKPTYETNKLEAYSTDSPISDDDSMDEYISNYKFDINLNEIILSLSINQGI
                     YNSKKEHVTSFMISDNNEYIFCTKKAIAYSIDWDKKSVRKKKLSEAKVNDWFLFVDDY
                     LNEEGELVINVIQKLLELNLLPSHYLDDYKLTNSWKSELKNYISYSKYNYNDVSNSLA
                     VLGEKINPITIKNWVTNKRLIGPIYLKTFEKIFKVIDSKLDHTEVYDATKSIRSLHTK
                     IKKSIDTFVSDNFFSSKSDIIKDEVSAVVLSMLGNTNDYVTPVQIREIHSCDKYLSPH
                     LVNKVLDLYSVY"
     gene            2624..6151
                     /locus_tag="PC399_002511"
     CDS             2624..6151
                     /locus_tag="PC399_002511"
                     /inference="COORDINATES: protein motif:HMM:NF012493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDF2593365.1"
                     /translation="MNCSELRDKTLDLVRKELLGPGSEDLCENIKYEIITDSPIERYS
                     TGILYPLNNNIDVSNTSEYDGEDNVFTSDDTQNELDRAISMSNETLPSSMGMTFFLKK
                     DAEVINFRVNYATYRNALYTDCHVKYTGDDFYSSNTINEYVELRGSFLYLKQKLTSEK
                     VYNLLKKHDLLDNAEAKFVLYKLANQCPDDENKTGGYVRVPHENLIVSIDMDKNYIKK
                     PISKDLDIVLSKRIYSNDFVSITCMLVNSQSGPKKYNNTIFQPELMVLAEDNNDLEFI
                     DIFTAGSVNTFKKLDSEEISLDLLYRNKKTFATGHGVAIDQDIQNDALIKLCTEFIPH
                     FEVPKLDFNVDELSDVSRELLSMKNLSDISDLSKDKKIYFLYKFVDAYDIWIDDLEED
                     SKDLSDVFKDVAYEHIKNCRDTKSRMLEGINLIKSNDEVYRAFSLMNRAILMQRCHSI
                     HITDRLPGSNNLDYIDYSNDNSAWRPFQLAYILTCLSSIENPDSKHRDLVDLLWIPTG
                     GGKTEAYLGLSAFTIFLRRIRNKENGNGTAIIMRYTLRLLAAQQFIRASILICACEII
                     RKENKSELGKKEISIGLWIGSDQTPNFNDEAEEMLNNLTGNNIKFRSSINSEYELDNL
                     KSKYNKFQLLKCPWCGTKLVKEWDKTSKKAVGDWGYRIKSRPKKFYLCCPEEDCSFED
                     MLPIQVVDEEIYNNPPTLLFGTVDKFAMITWTTEVASLFALDDDNSNLSPELIIQDEL
                     HLISGPLGTMVGLYETALDAMCSYKGIKPKIIASTATIRKAQNQVKNLYNRNVAQFPP
                     PGLTSSNSFFVKEMPLSKKRGRLYVGVIPSGKTQVTVEVRLMSALLQRLKMMENVELN
                     LLDKYWTLVSYFNSIRELGKCSTLLNDDIKDNMERLCKRVSAHPYQSLRNLNEQRELT
                     SRLTSTKINTTLDELSNTLTSNNLTVDTLLASNMISVGVDVDRLNLMVVVGQPKLTSE
                     YIQASSRVGRSNEGLIFTLYNPTKTRDRSHYEQFFSYHQSFYKFVEPTSITPFSEPAR
                     ERGMHAVLISMIRHILGLQANDLASRFSKNMDGVEDLKNFIIDRSIDIFRENNQNADD
                     NIVNEFKSEISSELDIIMDLWHDKAQISDKDNLLHYYKRPSKFDNKIYNNLLIPYDKD
                     VITNEKQTLNSMRNVDKQCTVEIIELEEN"
     gene            6153..8066
                     /locus_tag="PC399_002512"
     CDS             6153..8066
                     /locus_tag="PC399_002512"
                     /inference="COORDINATES: protein motif:HMM:NF020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HDF2593366.1"
                     /translation="MSIKLDDKTHNLRINQATSVFGPGAMLDFIDQTLMTAHPNYWTN
                     TIKIQDDRLQATLRVNELRTPPTIDDRSSVPFVRFPRWYFCPKCRKFMPIEDWEKLYK
                     NAHKDKPMKIPICLTCSTKKSTKQLVPTGIITICEHGHIDDFPWVKWVHFRNKSGRKE
                     ICENPQILMKTGKSNLGLEGIELECSCGAKANMKGAFMRLKSSKSNDYGVPSEYKEMF
                     KCSGNSPWYNKKVNKCTKYSFASQRGASNVYYPKIESSIIIPPYSDTLNSKVLSSKGY
                     DTFKKFLDRAIKKNKLERFMDEDFEDFASDIAKETGFDTDKVSIVLKDLLPFEDSSPS
                     TTTRNQYREEEYEALIGNIDAKSKKTNDFTIEEQNLSDYDIPNFSKIVLVKRLREVRA
                     LTGFSRVNPPDNNIFGEEDIDDSCNDTKVISLRSRDCKKSFYPASEVRGEGIFIEINN
                     DMVKSWISSNPKIQDNASILNSRYNKDINVNDKAYRKITPKFLLLHTLAHLIIRELSF
                     VCGYATSSLRERIYCNNSENEKVMSGILVYTASGDSEGTLGGLVRQGMHDSLPSILNS
                     ALNRALWCSNDPICIDSTGQGRNSLNLGACHSCTLLPETSCEEYNLLLDRSMLIGDMD
                     NRDIGFFKNLILI"
     gene            complement(8772..9515)
                     /locus_tag="PC399_002513"
     CDS             complement(8772..9515)
                     /locus_tag="PC399_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593367.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKKIKIKFGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(9537..9851)
                     /locus_tag="PC399_002514"
     CDS             complement(9537..9851)
                     /locus_tag="PC399_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593368.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(9855..10055)
                     /locus_tag="PC399_002515"
     CDS             complement(9855..10055)
                     /locus_tag="PC399_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2593369.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            10202..10963
                     /locus_tag="PC399_002516"
     CDS             10202..10963
                     /locus_tag="PC399_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2593370.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDIK
                     EIIKNDVLTKQSIDELLDIGLSEESIETIYDYFSDSTDNGSNIRLKKLISQKENITIK
                     NNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            11020..11218
                     /locus_tag="PC399_002517"
                     /pseudo
     CDS             11020..11218
                     /locus_tag="PC399_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11215..11706
                     /locus_tag="PC399_002518"
     CDS             11215..11706
                     /locus_tag="PC399_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2593371.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            11798..12550
                     /locus_tag="PC399_002519"
     CDS             11798..12550
                     /locus_tag="PC399_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2593372.1"
                     /translation="MNKSYIPKYTHKFPTNIPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILINNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            12540..13706
                     /locus_tag="PC399_002520"
     CDS             12540..13706
                     /locus_tag="PC399_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2593373.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPLRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKYLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQGPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYINNNYVFCNELGIPIDDKRPLRNLKSILNSLNIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(13822..14052)
                     /locus_tag="PC399_002521"
     CDS             complement(13822..14052)
                     /locus_tag="PC399_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593374.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(14058..14537)
                     /locus_tag="PC399_002522"
     CDS             complement(14058..14537)
                     /locus_tag="PC399_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HDF2593375.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFDSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            complement(14537..14752)
                     /locus_tag="PC399_002523"
     CDS             complement(14537..14752)
                     /locus_tag="PC399_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HDF2593376.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            15258..16409
                     /locus_tag="PC399_002524"
     CDS             15258..16409
                     /locus_tag="PC399_002524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593377.1"
                     /translation="MKKLTIRLKDDFHKDLLDLVAFKNDSINSYVINLIKNDLSKNTN
                     LLRSYDNMIRDNITNVLITQKINELKAIQDGIFKEYKVNDILSNSKIYEVMTANSLNH
                     ILIPGHSGSRDAKDEFGNEFEYKHFKKSSSNHSWTFNDFSDTSIAKLKKAKAVIFAHI
                     NDVDFPYPGKLDWYYSIPGDIMCEYLLKYTQSIQNSRKMINVSPNQLESRLNASKIIP
                     SAQNGVYDKYLSAIYKASSELEELTGVKNLLTSNKFWELIVALELNHQVNPEQGGREG
                     AHDAYDSEGNPYEYKVSKNHAWNFQDISDNVLNKYYNDKAIILAVVDKENIEVEHIYS
                     TEPADVVPLLRKKLQEKINRCLEKGSPIRRLQVSLSKSDLTRVNAKKLY"
     gene            complement(16534..18153)
                     /locus_tag="PC399_002525"
     CDS             complement(16534..18153)
                     /locus_tag="PC399_002525"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDF2593378.1"
                     /translation="MDKINKLKERIANIEATNKNDIYSSNLYWSQKPFNICDEIIEHL
                     SEDGDIIFDPFMGSGVTVIEALKKEYKRNAIGCDINDAPIFIVKTLLEKYNVPKIEKD
                     IKELNNKLSKLSTYYSTKCRECGEVVTISKTIFDKENRNAKCELKSIHYKCKECKNDF
                     KGEPNETEISKFFIDEELKNIDDFQFIANSRLAVGEGDTVRTIFSNRNLIVLDKILGH
                     IDEYKNSESYEPMKYILLSILHLSKITDTHSNSQWPLWIPKKDCVEKNVVELFIKNSK
                     KTIKALKLASKYLCENRENVELIKNLDRGKFKLIKDGVQNIEDDVIPNEFVDLIITDP
                     PYMGQVLYSEYMQLYKPFMDLEFELENEIVISSAPARDKGKVLYFDMMNSAFENISRV
                     LKVGKYMCMYFHDSNLDVWDNIIDIMTNNNLKYMGQIHVSKNKNTLKNILSPKKSLNG
                     DCIIFFKKDLNINYDINEVDNIEDKINNIAQAIIDIKGYASTPELYDNGALEYIISNG
                     KLKEISKKYKDLTSIFEQRFNWDKERGVWTV"
     gene            complement(18156..19448)
                     /locus_tag="PC399_002526"
     CDS             complement(18156..19448)
                     /locus_tag="PC399_002526"
                     /inference="COORDINATES: protein motif:HMM:NF014177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDF2593379.1"
                     /translation="MADIFTVLNYQGSKKRLLDFIYENTQEYIDSDKAFLDICSGTSA
                     VAYNMKRHFTVYTNDSENYAYQISNSIVKNNERYNFSQSDEMIIREKYNINLQRLNNI
                     FNDMVNQEDMLLEQEDADGLIELYSNCPCIWKNNVDIEIEGNTIQSIYDLRKCKRDIP
                     NVLFTLYYSSSYFGIKQSMEIDSIRYAIEFIESEITKSILFTSLFFAMKECVFAKDGH
                     MAQPLNMETGKKRMLRLKARSIYDIFIDKLKEFNTEEFVLSQKENKCFNKDFRTLLET
                     EESIKDEVGFIYIDPPYTDMQYSRYYHILNTVVLYDYPEISLSRGTISKGIYREGRYQ
                     SPLSQRKHALNEIDFLFSFCKENKINVALSYAYPIDTEKQATNRYTASIDDLINVAKK
                     HFGDNVKVVHEEYSHANNRNSDKKNVLEYLIMGIHNKE"
     gene            complement(19594..21159)
                     /locus_tag="PC399_002527"
     CDS             complement(19594..21159)
                     /locus_tag="PC399_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2593380.1"
                     /translation="MTGKKAVVYARFSSDNQRDESIDAQLRAINEYADKNNIKIVNQF
                     IDRAKSATSDKRPEFQNMIKFCEADTTGISMVIVHKLDRFSRDKYDSAMYKQKLKVKG
                     IRVVSVLENLDNSPESLILESVIEGMAQYYSANLAREVAKGQKENALKALHNGGDAPL
                     GYDVAHDKTYTVNEEEAQAVKIIFDRYIKGYSYSKIIDELNILGYKTKRGNKFGKNSL
                     HGILNNEKYTGVYVFNKTQRKGVDGKRNGHKQKSEEEIIKIEGGMPQIIDKGVFKQAQ
                     DMIQKRKKTPGAHKATTTYLLTGLIKCGECGHAFQGNKRKDSYGNEYVSYRCGCRKQK
                     RECNNREIKRDYLEEFILQELEKNILNDEAIPILSKALNEKLNDKNESNAELLKNLEQ
                     KLDKVNKEISNILNAIMNGIVNSMLKDKLDELEQVKFNLDLKMNELKIENKVVDGVGV
                     TEDQIRGMFSRFKEFVLERNIPECKKFIGDYIKEVIVYKDHIEVIFNVVFCFVDNDIT
                     HDLHIITERGKIT"
     gene            complement(21246..21401)
                     /locus_tag="PC399_002528"
     CDS             complement(21246..21401)
                     /locus_tag="PC399_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593381.1"
                     /translation="MTETYKISIGRKDLYSKDYKVVERTDYEKRHTVNISRSLVDILS
                     ILVKEDR"
     gene            complement(21464..23227)
                     /locus_tag="PC399_002529"
     CDS             complement(21464..23227)
                     /locus_tag="PC399_002529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593382.1"
                     /translation="MSKAINREELIKLYEEIGLNVLDNNTVMNSSNKKICNFLVNIIE
                     QRVIDGKMYLCINAMGINGELLSEVILDSNTINRTTWVIENWGFKYSIDKKYINDFIE
                     LIQHLCMNIKTKYIYDHTGWIYQDNKWIYLHGGGVIGGSDVQVELDESIDNYILPDKI
                     SDINKACEMSLKLVDLIEDDKGIIFSSLVYLSPLIEIISRKIKPPENIVWTYGKTGSR
                     KTAVSKVVLSHFGDFNKKIPATFNDTITAIELKANKLKDSLYLCDDFAPKQDFRDKKI
                     QDSKAESIIRMYGDRVGKGRSSNKLDIKDAKRPRGMILITGEDVICGESSNARLLSIE
                     LNRSSVDLNILTELQNNSHLLGEAMRGYIEWLLSEMGNIDELSNILLYNFKSYRDEIR
                     EQYKDDIHGRTIESSAWILVGFSCMLNYIRDKGVIDKYEDDEYMDRAIKVVERLIEEQ
                     IKLISRNSPIDIFLNTLKESINSKSIRIATLNDQNQVIEDKLEYICGYKDKEYYYFYT
                     DKVYSFIEFENKKRGIHLGISQRQLVKQLRDSGIVKVDSNNDSPKKTIHTKDASDSTG
                     YTTIRPRMLQIERSIIENFQI"
     gene            complement(23239..23556)
                     /locus_tag="PC399_002530"
     CDS             complement(23239..23556)
                     /locus_tag="PC399_002530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593383.1"
                     /translation="MTNFKSVKVELILLIECEEGNRNELEGMIDEGVLNEKEYSLTIL
                     GSTEYEDNARAIYILMNTGGSYEKNLQRLSRLHLKIENLLKDTNVKYRGISLVPNIVK
                     WDK"
     gene            complement(23746..25107)
                     /locus_tag="PC399_002531"
     CDS             complement(23746..25107)
                     /locus_tag="PC399_002531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593384.1"
                     /translation="MKGNINKKDIDLSKITFDKFLEDEGLQNRERDYLYVDDYDNTLD
                     TQEFKLHIIEENTDNTYIDKDKYWYLDLDDSLFEYEEDEDTIAKTLYSLIDIRIKFLK
                     HVEYRKYINLGVIDEEYFDEIKSFLKFYIVAKTKKNELGNSNLKQMAIDIFDKERFED
                     DISDDDMENISEWIGEDQNFKMFYDKVCRDLSNSTQDKEILKLAGIRNNKVKHYKGRK
                     ISRKCKLELKSLTKMWEYETKLTHDANKAHKKIKSNMLEKMTDKNYKNIYEYLNNEII
                     NSNEEYKNIYFYSLELKLANELRKCTLKNLNKIKDIENQVKFISYTIAYIHIVPVLTI
                     REKLSDKLFNILSTNEGKDIEKFTKEVSLIMRIINIECLELLNIVSKNNYYKNFSEKE
                     LEEFKSIYNINNGRKSFENKYKIKVDYNIDDVRKYGEVIKAINIESKKKIVDRIKKKR
                     KSN"
BASE COUNT         8884 a         3633 c         3019 g         9587 t
ORIGIN      
        1 atatccatta tattcttgat aatgatatat gtgaaattta ttttaataaa caaaggctaa
       61 caaattctaa gtattatcaa gataatatta atgtattaaa ttatattata aatgaaaaaa
      121 ataataactt aattatcaat tataatgaca aatacatata tgctatttat ttgttgaata
      181 tagctataaa ctcatactta aataatacat taaacccaaa taatcacatt cttacaaaac
      241 tcgataaagg tatgcccctg ctctacaagg gtcaaatagt tatatttaaa aatataaata
      301 atacacacat aactttagga cataaaaata aaacggatac aacacttata cctattgact
      361 cggcttatat gcttataacc tataatggtc atttagaagt caattctatg ggcaaaactc
      421 cagcatctaa gacttttcaa acaaagaatg ttctatcatc tttaggtttt tcagacttaa
      481 aaaatatgac tggagtaata aatgattcta cactgattat attgccttgt aaagatgata
      541 tatctgactt agttagtaat attaaaataa aagatattaa taatacttat aagtttactg
      601 aactatttcc atgttcatat atttcatcaa ctggtgccga aactgattat cctggaaatc
      661 acgctaagca aataccttta cttaaattta caactaatat ttctagtgca tttgaaatta
      721 ttaaatctga taagtctata aaaaatgtat ttataatagg tgattatcca atttataagc
      781 atataaatga ttttgaaaga attttaaata gaaaaaggat agaaagtgta aatattgtaa
      841 cctcaaattc aaatataagc aatatattag atttaccaaa tatagataat ctaaatatat
      901 actcttggtc taaagatgtt ctgttaacct atagccaaga ttttgaaaat acctctactt
      961 ttcagaaatt atggctagat aaacttataa ataaaaatat atgtactact tctgttgatt
     1021 ctaatatctc tgacttgata catcaggcta gaaaatcact ttattacata tctaagtatg
     1081 attttgatat ttccataaaa aactcactta tgatgtgtag ttactctctt cttaaaatct
     1141 tagaaaatac accttttaac ctagattttt tagaagaaac tataaagctt ttaaatttaa
     1201 atatagcaac tccttcagcg aagctagaaa atataaaata tttaatttca agtactagcc
     1261 cagatttaga attaagtgca ttatttgatg atgttatagt caaatttgaa agaattataa
     1321 atgaattatc tgataataat accaaattca atacactaaa taaattagta tcttcatata
     1381 aatacaaaac atttacacca aatacacctg ttatattgct acaaaaacaa tatgaagcta
     1441 ttatacttaa aaatttatta aaatctagaa atataaattt acaagttatt tctgctgaaa
     1501 aatttaaaga ttcaaaaaca ataaaaaatc ttattatgtt tggattctat gatttgaaat
     1561 atttagatat tttaaatagt gatttgatta taaatattac actgtattta tataatagtg
     1621 aattatccaa atataattac tatatgaatc aaataaattc tgcaagaaat aaaattgaaa
     1681 aaaataataa aatatataac ttacttgata taaaaccttt taaaccaact tatgaaacca
     1741 ataaattaga agcttattct accgatagtc ctatttctga tgatgattct atggatgaat
     1801 atatatctaa ttataaattt gatataaatt taaatgaaat tatattatct ctatctataa
     1861 atcaagggat ttacaactct aaaaaagaac atgttacatc attcatgatt tctgataata
     1921 atgagtatat attctgtaca aaaaaggcaa tagcctattc aatagattgg gataaaaaaa
     1981 gtgttaggaa aaagaaactt tcagaggcta aagttaatga ctggttttta tttgttgatg
     2041 attacttaaa tgaagagggt gaattagtta ttaatgttat acaaaagcta ttagaactta
     2101 atttattgcc atctcattat cttgatgatt ataaattaac taattcatgg aaatcagaac
     2161 ttaaaaacta tatatcttat agtaaatata attataatga tgtatcaaac agtttagctg
     2221 ttttaggtga aaagattaat cctataacaa tcaaaaactg ggtaacaaat aaaagattaa
     2281 taggtcctat atatttaaaa actttcgaaa agatatttaa agttatagat tctaaattag
     2341 atcataccga ggtatatgat gctactaaaa gtatacgctc tttgcacact aaaataaaaa
     2401 aatctataga tacatttgta tctgataatt tctttagcag taaatcagat ataattaaag
     2461 atgaagtgtc tgcagttgtt ttaagtatgc ttgggaatac aaatgattat gttacacctg
     2521 tacaaatacg agaaatacac agttgtgata agtatctgtc accccacctt gttaacaaag
     2581 ttttagactt atatagtgtt tattaaataa ataggaggta aatatgaatt gtagtgaact
     2641 aagagataaa accctagatc ttgtaagaaa agaacttctt ggacctggtt cggaagatct
     2701 ttgtgaaaat ataaaatatg aaataataac agatagtcca atagagcgtt attcaacagg
     2761 tattttatat ccacttaata acaatattga tgtaagtaat acatctgaat atgatggtga
     2821 agataatgta tttacatcag atgatacaca aaatgaacta gatagagcta taagtatgtc
     2881 taatgaaact ttaccttctt ctatgggtat gacttttttc ctaaaaaaag atgcagaagt
     2941 aataaatttc agggtaaatt atgctactta tagaaatgct ttatatacag actgtcatgt
     3001 taaatatact ggtgacgact tctattctag caatacaatt aatgaatatg tagaacttag
     3061 gggatcattc ctatatctta agcaaaaact aacatctgag aaggtttata atttactaaa
     3121 aaagcatgat ttacttgata atgctgaagc taaatttgtt ttatacaagt tagctaacca
     3181 atgccctgat gatgagaata agactggtgg ttatgttcgt gttccacatg aaaacttaat
     3241 agtatcaata gatatggata aaaactatat taaaaaacct atatctaaag atttagatat
     3301 agttttatct aaaagaattt attcaaatga ctttgttagt attacttgta tgctggttaa
     3361 tagtcaatct gggcctaaga aatataataa tactattttc cagcctgaac taatggtatt
     3421 agctgaagat aacaatgatt tagagtttat agatattttt acagctggat cagtaaatac
     3481 ttttaaaaag ttagattctg aggaaatatc tttagactta ctttaccgaa ataaaaaaac
     3541 ttttgctact ggtcatggtg tagctataga tcaagatatt caaaatgatg ctttaataaa
     3601 actttgtact gagtttattc ctcattttga agtacctaaa ttggacttta atgtagatga
     3661 attaagtgat gtttctagag aattactgtc tatgaaaaat ttatcagata tctctgatct
     3721 tagtaaagat aagaaaatat attttcttta taagtttgtt gatgcttatg atatttggat
     3781 agatgatcta gaagaagatt caaaagatct tagtgatgtt tttaaagatg tagcatatga
     3841 gcatataaaa aactgcagag atactaagtc tagaatgcta gaaggtataa atttaattaa
     3901 gtctaatgat gaagtataca gagcattttc tctaatgaat agagctatac ttatgcaaag
     3961 atgtcattct atacatatta cggatagact ccctggatct aataaccttg actatataga
     4021 ttactcaaat gataattctg cttggagacc attccagctt gcttatatac ttacatgttt
     4081 atcttcgata gaaaatcctg actctaaaca tagagattta gttgaccttc tttggatccc
     4141 tactggtgga ggtaaaacgg aggcctactt aggtctatct gcttttacta tcttcttaag
     4201 aagaataaga aacaaagaaa atggtaacgg tacagctatc ataatgagat atacactaag
     4261 gctattggct gcacaacaat tcataagagc aagtatatta atttgtgcct gtgaaattat
     4321 tagaaaagaa aataagtctg aacttgggaa aaaagagatt tctattggtt tgtggattgg
     4381 gagtgatcaa actccaaact ttaatgatga ggcagaagaa atgcttaata acttaacagg
     4441 taataacatt aagtttagat ctagtataaa tagtgaatat gagttagata atttaaaatc
     4501 aaaatataat aaattccaat tactaaaatg tccttggtgt ggaacaaaac ttgttaaaga
     4561 atgggataaa acttctaaaa aagctgttgg tgattggggc tatagaatta aatctagacc
     4621 taagaaattc tacttatgct gtcctgaaga agattgttca tttgaagata tgttacctat
     4681 acaagttgta gatgaggaga tttataataa ccctccaaca cttttatttg gtacagttga
     4741 taaatttgca atgataactt ggactacaga agtagctagt ctttttgctt tagatgatga
     4801 taatagcaac ttatctcctg agttaataat tcaagatgag cttcatctta tttctggacc
     4861 tttaggtact atggtaggtt tatatgaaac tgcacttgat gctatgtgta gctataaggg
     4921 tataaaacct aagattatag cttctactgc tactataaga aaagcacaaa atcaagttaa
     4981 aaatctatat aatcgtaatg tagctcagtt tccacctcca ggattaacat ctagtaactc
     5041 tttctttgtt aaagagatgc ctttaagtaa aaaacgtggc cgactatatg taggtgttat
     5101 tccatctgga aaaactcaag ttactgtaga ggttagactt atgtcagcat tactccaaag
     5161 acttaaaatg atggagaatg tagagttgaa tttattggat aaatattgga cactagtatc
     5221 ttattttaat agtatcagag aattaggtaa gtgtagcaca cttttaaatg acgatataaa
     5281 agacaacatg gaaagattat gtaaaagagt ctcagcccac ccttatcaat cattaaggaa
     5341 cctaaatgaa caaagagagc ttactagtag acttacttct actaagataa atacaacttt
     5401 agatgaacta tctaatactc ttacttctaa taatttaact gtcgatactc tattagctag
     5461 taatatgatt tcagttggtg ttgatgttga tagattaaat ttaatggtag tagttggtca
     5521 gcctaagtta acatctgaat acatacaagc tagcagtaga gttggtagaa gtaatgaagg
     5581 acttatattt acattatata atcctacaaa aacaagagat agatctcatt atgaacagtt
     5641 ctttagttat catcaatcat tctataaatt tgttgaacct actagtatta cacctttctc
     5701 tgaaccagct agagaaagag gcatgcatgc agtattaata agtatgatta gacatatttt
     5761 aggacttcaa gctaatgatc ttgcaagtcg attttcaaaa aatatggatg gtgtagaaga
     5821 tttaaaaaac ttcataatag atagatctat cgatatattt agagaaaata atcaaaatgc
     5881 tgatgataat atagtaaatg aatttaaatc tgagatttca tctgaattag atattataat
     5941 ggatttatgg catgataaag ctcaaatatc tgataaagat aatttattgc attattataa
     6001 gcgccctagt aaatttgata ataaaatata taataatctt ttaattcctt atgataagga
     6061 tgttattaca aatgaaaaac aaacattaaa ttctatgaga aatgttgata aacaatgtac
     6121 tgttgaaatt atagaattgg aggaaaatta atatgtctat aaaattagat gataaaactc
     6181 ataatttaag aataaatcaa gctacaagtg tttttggacc aggagctatg ctagatttta
     6241 ttgatcaaac attaatgact gctcatccaa attattggac taatacaatt aaaatacaag
     6301 atgataggct tcaggctaca ttgagggtta atgaactaag aacacctcca actatagatg
     6361 atagatcttc tgttcctttt gttaggtttc ctagatggta cttttgtcca aaatgtagaa
     6421 aatttatgcc tatagaagat tgggagaaac tttataaaaa tgctcataaa gataaaccta
     6481 tgaaaatacc tatttgcctt acttgtagca caaaaaagtc tactaaacaa ttagtaccta
     6541 ctggtataat tactatttgt gaacatggtc atattgatga ttttccatgg gtaaaatggg
     6601 ttcattttag aaataaatct ggaagaaaag aaatttgtga aaatcctcaa atattaatga
     6661 agactggaaa aagtaaccta ggattagaag gtatagaact tgaatgtagc tgtggagcta
     6721 aagctaatat gaagggcgca tttatgcgtt taaaatcttc taaatctaat gattatggtg
     6781 tacctagtga atataaggaa atgtttaaat gctctggtaa ttcgccttgg tataataaaa
     6841 aagtaaataa atgcactaaa tattcatttg cttctcaaag aggtgcctct aatgtatatt
     6901 atcctaagat agaaagttca attattatac caccatactc tgatactctt aattctaaag
     6961 tgctatctag taaaggttat gatacattta agaaattctt agatcgtgct attaaaaaga
     7021 ataagttaga acgctttatg gatgaggatt ttgaggactt tgccagtgat attgctaaag
     7081 aaacaggttt tgatactgat aaagtatcca tagttttgaa agatttattg ccttttgaag
     7141 attcttcacc atcaacaaca actagaaatc aatatagaga agaagaatat gaggctttaa
     7201 ttggaaatat agatgctaaa tctaaaaaaa ctaatgactt tacaatagaa gaacaaaatc
     7261 tatctgatta tgatattcca aacttcagta aaatagtgtt agtaaaaaga cttagagaag
     7321 taagggcatt aactgggttt agtagagtca atccacctga taataatata tttggtgaag
     7381 aagatataga tgatagctgt aatgatacta aggttataag tttaagatct agagattgta
     7441 aaaaatcttt ttatcctgca tctgaggtta gaggtgaagg tattttcata gagataaata
     7501 atgatatggt aaaaagctgg atttcatcta atcctaaaat acaggataat gcatctattt
     7561 taaattctag atataataaa gatattaatg ttaatgacaa agcttataga aaaattacac
     7621 ctaaattctt attactacat actttagctc atttaattat aagagaactt agttttgttt
     7681 gtggatatgc tacctcttca cttagggaac gtatatattg taataattcc gaaaatgaaa
     7741 aagttatgtc tggaatttta gtttacactg caagtggtga ctctgaaggt actttaggag
     7801 gattagttag gcaaggtatg catgattcat taccatctat attaaatagt gctcttaatc
     7861 gtgctctttg gtgttctaat gacccaatat gtattgatag tactggtcaa ggtagaaatt
     7921 ctttaaatct aggagcatgc cattcatgta ctttattgcc tgaaactagc tgtgaggagt
     7981 ataatttatt acttgataga agcatgttaa ttggtgatat ggataataga gatattgggt
     8041 tctttaaaaa tctaatttta atttagtaat aactaatttt ttatttatat aactaaaggt
     8101 taaaagttaa ttttatgact tctatccttt agttttttgt atttaaatta ttattttttg
     8161 caaatcattc ttactagaaa ttacttatta taactaattc ccaataatta tataattttg
     8221 tgctcatact gaaattttta aataaactat taatttatat tatatatgat ttttcaaaat
     8281 aaaaaacata ttaactatta aatcaatatg ttttcgtgga ttccaatggt ggagaccgag
     8341 gagaatttat aagtggttat ttgattgttt tgttggtttt tataagtata ggtatatggc
     8401 tatttggtca tgatgttgaa gcaatttttc tggttatttt ttattgtcta tttttgtagt
     8461 tggggtatta ttggggtata attttctact ttccaaaatt attgatactt caataatata
     8521 aattaaattc ttagttaagt tttttaatga ataggtaaat attatttgta ctcataacaa
     8581 tatagtttac gcataatttt atatatattt tctggtatat ctgcaactaa cttagtgtaa
     8641 tttaaacgat attagagata cttaaaaaac aaatatatga aagatttata actaacttag
     8701 tgtaatttaa ttgattgctc tgcttctgta tttttaatta agttgtttga attatttata
     8761 atataacctc cttaccactt taattgtgag tcatctggat tatatattcc tgtctcttta
     8821 tttatgacaa tttccttgct attataattt ccctctagta tcttagtaaa attacttgaa
     8881 ttcattatcc aatcaaaatt tgctttccat cctgtattat tatttccatt cagaaaactt
     8941 gttttactaa ctatatttat aacctctaat acagtttctt tatcatattc ttttattctt
     9001 gaatttattc tcctctttct tgtatctgtt agtttcttga cctttgaaag ttcaatacaa
     9061 gttgagttaa atttgcttat gatttcacta tatataatat tattttcatt ctttatattc
     9121 ttgattgttg ttgactgatt gttaatagtc tgttgattag gttgttgatt ttctatattc
     9181 tcttgataaa gctcccagtt ttcaatagtt attactatat acttgtttgt tgtttcgtat
     9241 gttagaaaac cagcattttt aaaaaccaac aaacaacttc taaccatttg ataactaact
     9301 cctttaccac atctttcagc tattttgcta tatgaagaaa taaattgtcc aaactttatt
     9361 tttatctttt tattatctat aaaaacatga ttttctttat gactagccat aagtaataga
     9421 gttattaata ttacctttct ttctggttta atatctttcc aaattgattt atccaacaac
     9481 tccctataaa gctttatcca ccctttattg ctcaattgca aacacctact ttaagactat
     9541 aacttgatac catttgcatt ttcaataaac caatcatcta atttattttt tattatgtat
     9601 actcgattat ttatccatat acaagggaat cctttgtttt tagaatgaca taactgccta
     9661 aattttgatt ctcctatagg ataaagctta gatgcttctt tacatgttat aagctcagct
     9721 ttatttcttt tcgcattttc aataatttta tatccttgct ttttttcttg tgcttcttct
     9781 tcattcttta caataatgtt taaagtggct tgtaatgtct ttaaaacctc taaattatta
     9841 ttctctttca tattttaatc ctccttacta aaaaataatt cttctactgt tgtatctaaa
     9901 gctctagata gtttaatcat caaatcattg cttggattat tagccttacc tgtttccaag
     9961 tttgataaat aatatcttga tataccaact ctatctgcca gttcttgttg ctttagacct
    10021 tttttaatcc taaatatttt taaattgatt cccatacttt ttcctccttt tttctagttt
    10081 acattcgtaa actttacatt ttaattataa tgtcgatata tgtgtactgt taataagaat
    10141 atattatttt tctgttctca tttgtgtact tttgggatat actttatacg aggtgataat
    10201 attgcaaatt ggaaaaaact taaaaaagat aagaaaacaa aaagagctaa cgcagattca
    10261 acttgctgaa attagtggca tctcaagaaa tgctctaata aattatgaaa atgataaaag
    10321 aatacctagt atagatacct tatcaaaact cgctaaagct ttgaaaattg aaaagactat
    10381 cttttatttt gatattgaaa atgaagtaat gactaatgaa gatgttttat accttttaca
    10441 gtctttattc ccagaagaat actattcttt tgatgaattt ttaaatgcta tttcaaatct
    10501 tatggaagat gatattagaa ttacctcaag tttattgcta ggtgcctggt ctgataaaga
    10561 ccaatattat aaacttggtg aaaaattaga actaaatgaa aatcaagttt ataattggat
    10621 actttcagat tgtatatcta atactgaatt agatttttca ttacatcctg atgacataaa
    10681 agaaattatt aaaaatgatg ttcttacaaa acaatcaatt gatgaattat tagatattgg
    10741 tctatcagaa gaaagtatag aaacaatcta tgattacttt agtgactcaa cagataatgg
    10801 aagtaatatt cgactaaaaa aattaatatc tcaaaaagaa aatattacta ttaaaaataa
    10861 tgattacttt aaattattac aagaaaataa cgaactaaag gaaaaaaatg aaaagcttga
    10921 aaatacactt aaagaaataa aaaaactact aactaaaata taaaataata tcaatactat
    10981 ctaaagcaat gataaaaaac tatgaatgga gttacaatta tgaataaatc atatatgcta
    11041 aaatacacat ataaatcccc tactaattta tttcatggag ataaaaaata tactctaaca
    11101 tacactaaga gagttatgag taatttagta tatgatatgg gataatctag aaaataactc
    11161 ctttacatgt ttagaagtac aatcattgtt tgatgatata tttaaggtga aattatgatg
    11221 aataatacaa ttggagaaaa gattaaacaa tatagaaaat cgataaatat gtcaagggat
    11281 gagctagcca acaagattgg ttgttcagtt catgctatag ctaaatatga gcaaaatcaa
    11341 agaatgccta gtttagatat gataagaaaa atagctactg ctttatcaat tgctataaat
    11401 aaactcattg ttgaagaaaa tgatttagat actttaacaa ataataatat taatttaaaa
    11461 gtatttgata aagagttgga tttagtaatt cacatattag aaaattatgg ttatgatata
    11521 aaaacagact ataaaaaaaa gtttgaaatt acagataatg cagatacaat aaaaccaatt
    11581 aacctaaact tctctgactt caaagacttc tccaaaaatc taaattgggt gataaatggt
    11641 cttgttgaaa attttataca agaaaactct aatggtctta ttgaagatga ggatgaagaa
    11701 tattagtatt gcacattttc aaaatatgag tttacctatt aacttttata cattcatagt
    11761 taataaataa aaattagata tgaaaggagc taaaatcatg aataaatcat atataccaaa
    11821 atacacacat aaattcccta ctaatatacc tcatggagat aaaaaatata ctttaacata
    11881 cattagaaga atgataagtg aatttgtata tgatatgggt aacctagaaa ataacccctt
    11941 cacattccca gaagtacaaa cattacttga tggtataacc gtaggcggac ataaattaag
    12001 tgaccaaaat caaatactaa atataaaatc atcatgggat tatgtaataa aattaagtaa
    12061 caaagaaaac ttatctttaa ataaagatac aatttgctct atacataaaa tagttgcaaa
    12121 agatgaagct ctaatcgttg gagatttcag aaatggtaat ataggtatag ctggtactac
    12181 tcaatatgaa tgcatagaag ccactctatt aaatgattta tttgtaaatg acataaatat
    12241 cataaataaa ataactaacc cattagaaaa ggctataata ataaatttat ggttgtccta
    12301 ttgccaattc ttctatgatg gcaacaaaag aactgctaga cttacctcaa acctaatact
    12361 tataaataat gatataggag ttctatcaat accagctaaa cataaacaag agtacaacac
    12421 attaatgcta aacttctatg aaacactaga agctgatgaa gttataaaat tcttattaga
    12481 gaaatgtatt acattctttg atggatataa ctataaatca tataaggagc tgtttaaaaa
    12541 tggcaactaa aagagctaat ggagaaggct ctatcggaaa atataaagat ggttggcgtt
    12601 cccgtataat gattggctat aatgaagatg gtaaaccact gagaaaagaa ttttatggta
    12661 agactcaaaa ggaagttaaa gataaattag atgcttataa aaaacaatat tatttaagct
    12721 ctgatatttc agatgataaa ataactttag agcattggtt ttacaattgg ttatttgaat
    12781 atagaataaa agatttaaaa cccaagtcat ttgaaagata tgaaagcatc tatcgtaatt
    12841 atattaaaga tactgattta ggcaatgtta aattaaagta cttaagagta tctcacatac
    12901 aaaaatatta caatagattg cttgattcca ataaaagtat acctactata aggcaaataa
    12961 acactaaact gaaaacctgc ttatcagaag ctgaaaaaca aggtcttata caacgtaact
    13021 attgtactat ggtaaatata ccagttgaga aaaaagaaaa caaacttgaa atattgactc
    13081 ttgaacaaca aaagagtttt ataaaagcta tagatgggca taaattagag gtattatttt
    13141 tacttgctct tggtactgga cttagattag gagaattatt aggacttaaa tggtttgata
    13201 ttgattttaa aaagtctaat ttaacagtta aaagaactct tcaaagaaca tattttatag
    13261 ataaaactgg aaatagagag cttaaagtat tagagcaagg gccaaagaca tcaaattctt
    13321 acagaactgt acctattcca aaagatgttt taaataaatt gaaagagcat aaagtaaatc
    13381 aaaatacaga tatattaaag gctggagaat tatatataaa taataattat gtattttgca
    13441 atgaattagg catccctata gatgataaaa gacctttaag gaatttaaag tctatattaa
    13501 atagcttaaa tatagagcct ataaagtttc atggtcttag gaaaacttat gctactagac
    13561 tttttgagaa tgatgttcct cctaaaactg tacaagtttt aatgggtcat tatgatatat
    13621 caataacttt gaatatatat actcaagtta tggaagataa gaaagttgaa gctgtagaaa
    13681 agttggataa aatattttca ttgtaaaaat caaatacata taaatattgt gatttcatat
    13741 agttaattac aaaatcaaaa taaggataca aattaatttt atattatatt gtatccttat
    13801 ttcgtatcta gttttaatta tttatcttgg atagccctta tggtacatta atccattttc
    13861 taacattaat tcttgagcca agtatatatg acccttacag tgcatatctg atacaccctc
    13921 acttataagt ttatatgttt cagaagtata taaataatcc atactttctt ccaagctctt
    13981 acctgtttgt ttggaaaatt ctaatatgat ttttttatat agtatttgta ataatgtttt
    14041 atttgcttcc ataatcatta tacctcctct tcatacctaa actttaaata tttatctatt
    14101 acattttgat tggttataca tatttgttca tttggtttat agtattttaa tctttttaaa
    14161 gcttcatctt tttcaataag attatcttga tacagttcta ttgtattgta aatcttatca
    14221 tcagcaatac cacctattat aatgtcatat ttcatatata tatcactcat atttctacat
    14281 tctattataa aatcaagcca ttctaaatta taactatcga agatttttac cttgcagttt
    14341 tcttttaatt tatcaatatc taaagaataa ctatttaaat atgctttttt acctcttttt
    14401 ttaaaacgct ctgtccagtt aattgcttgt gtctttatac tggttacata gaatcctttt
    14461 ccaaaatcta aagaatctct tgaaaaggta atactaggcg tctttaccac acaatatgaa
    14521 ccatgatata caatcattat atcacaccct tttctttaag tacttgcatt aaatcttcca
    14581 ctatgtaacc tttactttga gaatgtagtg cttcatagca aggtattata tagttatcta
    14641 aaatatcagt atcatgttta agaagtttat aaactccttc tgaatctata ttcaattttt
    14701 ctgctaaagc ttcaatgcaa aaaatagaaa attctagttt tctttcatcc attacaaacc
    14761 tcaactccta tcaagcttat ataatatata tttttactaa ttaacaactt aaactgtctt
    14821 acatttataa aaatattata tcataaagta gatattaaaa ttctatgtta cctattatca
    14881 taataaacaa ataaaataat aaacaaataa aataataaac aaataaaaac tttttataca
    14941 gcctaaatgc accccaatat acccccaaaa aaacctcatt ttctgtggtt ggggtacggt
    15001 tggtggattt cttctccaaa ataaaaaata ctagtcaaca aagactagta tttccaagta
    15061 tttcatcatt cttttttcaa aattggtgga gacgaatcgc aagaataata cccacgatta
    15121 atagtttagc tatttatcta tattgctata tatactttta tatatctata atttaatttt
    15181 tttaatcctt gactttataa acctgcttaa ttatacttat ctcgacggca catctaatta
    15241 atatccgagg tgatagcttg aaaaaactaa ctataagact caaggatgat tttcataaag
    15301 atttattgga tttagtagca tttaaaaatg atagcatcaa tagttatgtg ataaatctaa
    15361 ttaaaaatga cttatctaaa aatactaatt tattaaggag ctatgataat atgataagag
    15421 ataatataac aaatgtttta ataacacaga aaataaatga actaaaggct atacaagatg
    15481 gtatttttaa agaatataaa gtaaatgata tactttctaa tagcaaaata tatgaagtta
    15541 tgactgctaa ttcacttaat cacatactaa tacctggaca tagtggttct agagatgcaa
    15601 aagatgaatt cggaaatgag tttgaatata aacatttcaa gaaaagtagc tctaaccatt
    15661 cttggacgtt taatgatttt tcggatacaa gtatagcaaa attaaagaaa gctaaggcag
    15721 ttatatttgc tcatataaat gatgttgatt ttccttaccc tggaaagctt gattggtatt
    15781 actctatacc tggagatata atgtgtgaat atttactaaa gtatactcaa agtatacaaa
    15841 attccagaaa aatgataaat gtcagcccta atcaactaga gtctagatta aatgcaagta
    15901 agataatccc atcagctcaa aatggtgttt atgataaata tctaagtgct atttataaag
    15961 cttcaagtga acttgaagaa ttaacaggag ttaaaaattt acttacaagt aataagtttt
    16021 gggaattaat agttgcatta gaattaaacc atcaagtaaa cccagagcaa ggtggacgag
    16081 aaggtgctca tgatgcctat gatagtgaag gtaatcctta tgaatataaa gtttctaaaa
    16141 atcatgcttg gaactttcaa gatatatctg ataatgtact aaataaatat tataatgata
    16201 aagctataat attagcagtt gttgataaag aaaatataga agttgaacat atctactcaa
    16261 ctgaaccagc agatgtagtt cctcttcttc gtaagaagtt acaggaaaag atcaatagat
    16321 gtttagaaaa gggttctcct atcagaagat tacaggtttc attatctaaa agtgatttaa
    16381 ctagagttaa tgctaaaaaa ttatattaaa taatatcttt ggatagccaa tcctttaaca
    16441 tattgtataa aaattagaaa ttattaaata tttagtttac tataaaatat aaataaaaag
    16501 catctataat tatctataga tgctttttat ttattataca gtccataccc ctctttcctt
    16561 atcccaatta aatctttgct caaatatact tgttaaatcc ttatactttt tagaaatctc
    16621 ttttagtttt ccgtttgata ttatatactc tagtgctcca ttatcatata actctggagt
    16681 tgatgcgtat ccttttatat ctataatagc ttgtgcaata ttatttatct tatcttctat
    16741 attatctact tcatttatat catagtttat atttaaatct ttcttaaaga aaattataca
    16801 atctccattt aacgacttct taggactaag tatgttttta agagtgtttt tattcttact
    16861 tacatgaatt tgtcccatgt atttcaaatt attattggtc attatatcta ttatattatc
    16921 ccatacatct agattactat catggaagta catacacata tatttaccta cctttaatac
    16981 tctagatata ttttcaaatg cagaattcat catatcaaaa tataatactt tccctttatc
    17041 tctagctggg gcacttgata taactatttc attttctaat tcgaattcca aatccataaa
    17101 aggtttatat aactgcatat actctgaata taaaacttgt cccatatatg gtggatctgt
    17161 tattatcaag tccacaaatt cattaggtat aacatcatct tctatatttt gaactccatc
    17221 ttttatcaat ttaaatttcc ctctatctag atttttgatt agttctacat tttctctatt
    17281 ttcacataaa tatttagatg ctagttttaa tgccttaatg gttttcttac tattctttat
    17341 gaataattct actacatttt tttcaacaca gtctttttta ggaatccata aaggccactg
    17401 agaattagaa tgtgtatctg taatcttact taaatgtaaa atagataata aaatatactt
    17461 cataggctca taagattcac tatttttata ttcatctata tgtcctaata ttttatctaa
    17521 tactattaaa tttctatttg aaaaaatagt tcttactgta tcaccttcgc caactgcaag
    17581 ccttgaattg gctatgaatt gaaaatcatc tatattcttt aattcttcat caataaagaa
    17641 tttagatatt tcagtttcat ttggttcacc tttaaagtca tttttacatt ccttacattt
    17701 ataatgtata gactttagct cacactttgc attcctattc tccttatcga atatagtttt
    17761 cgatatagta actacttctc cacattcacg acattttgtt gaatagtaag ttgataattt
    17821 acttaattta ttatttagct ctttaatatc tttctctatt ttaggcacat tatatttttc
    17881 taataatgtt tttactataa atattggtgc atcatttata tcacacccaa tagcatttct
    17941 tttatactct tttttcaaag cctctataac tgtaacccca gatcccataa acggatcaaa
    18001 tattatatct ccatcttcac ttaaatgttc aataatttca tcacatatat tgaatggttt
    18061 ttgtgaccaa tataagttag aagaatatat atcattttta ttcgtcgctt ctatattagc
    18121 tatcctctct tttaatttat taattttatc catatttact ccttattgtg tatacccatt
    18181 attaaatatt caagtacatt ctttttatca ctatttctat tatttgcatg actatattct
    18241 tcatgtacta ccttaacatt atctccaaaa tgttttttgg ctacatttat caaatcatca
    18301 atactagccg tatatctatt agttgcttgt ttttctgtat ctattggata tgcataactt
    18361 aatgctacat ttattttatt ttccttacag aaactaaata agaaatcaat ctcatttaat
    18421 gcatgttttc tttgactaag aggtgattga tatctacctt ctctatatat cccttttgat
    18481 atagttcctc tactcaatga tatttctggg taatcataca aaacaactgt attaagaata
    18541 tgataatatc ttgagtattg catatctgta tacggagggt ctatatatat aaaaccaact
    18601 tcatctttta tactttcttc tgtttctaat aatgttctaa agtctttatt gaagcactta
    18661 ttctcctttt gtgataatac aaactcctca gtattaaatt cctttaattt atctataaat
    18721 atgtcatata tacttcttgc ttttaatctt aacattcttt tcttgcctgt ttccatattt
    18781 aatggctgtg ccatgtgacc atctttagca aaaacacact ctttcattgc gaaaaataaa
    18841 cttgtaaaaa gaatgctctt tgttatttca ctttctataa attcaatagc atatctaata
    18901 gaatctatct ccatagactg ctttatgccg aagtaactac ttgaataata caatgtgaat
    18961 aacacattcg gtatgtctcg tttacatttt cttaaatcat atatactctg tattgtattg
    19021 ccttctattt caatatcaac attatttttc catatacatg ggcaatttga atatagctct
    19081 attaaaccgt ctgcatcttc ttgttctaat aacatatctt cctgatttac catatcgtta
    19141 aaaatattat ttaatctttg taaattaata ttatattttt ctcttatgat catttcatct
    19201 gattgactaa agttatatct ttcattattc ttaacaattg aattagatat ctgatatgca
    19261 taattttcac tatcatttgt ataaaccgta aagtgtcttt tcatattata agctactgca
    19321 ctcgttcctg agcatatatc taaaaaagct ttatcagaat caatatattc ttgtgtattt
    19381 tcatatataa aatctaataa tctcttttta ctaccttgat aatttagtac tgtaaatata
    19441 tctgccaata gtctcacctc tttatcatat ttaaagtttt attataccat tttattttat
    19501 aattatataa atatattttt taaaactaat attttcaaaa ataattttta tctaaataat
    19561 atctgatatt tacttttata aaatatatat ttactatgta attttacccc tttctgtgat
    19621 aatatgtaaa tcatgagtta tatcattgtc aacaaaacaa aagaccacat tgaatatgac
    19681 ttcaatgtgg tctttatata cgattacttc ctttatatag tctcctataa acttcttaca
    19741 ttcaggtata tttctctcta gtacaaattc cttaaatctt gaaaacatac ctcttatttg
    19801 atcttctgtt acacctacac catctacaac tttattttct atctttagtt cattcatttt
    19861 taaatctaag ttgaacttta cttgttctag ctcatcaagc ttatctttta acatgctatt
    19921 tactatacca ttcattatag catttagtat attacttatc tctttattta ccttatctag
    19981 tttctgctct agatttttaa gtagctctgc attactttca ttcttatcat tcagcttctc
    20041 attcaatgct ttacttaaaa tcggtatagc ttcatcattt aatatgttct tttcaagttc
    20101 ttgtaatata aactcttcta aatagtctct ttttatctct ctattattac attctctctt
    20161 ttgctttcta catccacatc tatatgaaac atattcattt ccataagaat cttttctctt
    20221 attcccctga aatgcatgtc cacattcacc acatttaatt agtcctgtta atagatatgt
    20281 agtagtagct ttatgtgcac ctggagtttt ctttcttttt tgaatcatat cttgtgcttg
    20341 tttaaatact cctttgtcta ttatttgtgg cataccacct tctattttta ttatttcctc
    20401 ttcactcttt tgcttatgtc catttctttt tccatctaca ccttttctct gagtcttatt
    20461 aaatacatat actcctgtat atttctcatt atttaatatt ccatgtaagc tattcttacc
    20521 aaatttatta cctcttttag tcttatatcc taagatattt agttcatcta ttatcttact
    20581 atatgaatat cctttaatat atctatcaaa tataatcttt actgcttgag cttcttcttc
    20641 attaactgta tatgttttat catgtgctac atcataacct aatggtgcat cgcctccatt
    20701 atgaagggct ttaagggcat tttctttctg acctttagca acttccctcg ctaaattagc
    20761 actgtaatat tgtgccattc cctctataac tgattctaat attagggatt caggcgaatt
    20821 atctaagttc tctaatacac ttacaactct tattcctttt acttttaatt tctgtttgta
    20881 catagctgaa tcatacttat ctctagaaaa tctatctagc ttatgaacta ttaccataga
    20941 tattcctgtt gtatctgctt cacagaattt aatcatattt tgaaactctg gccttttatc
    21001 tgatgttgct gattttgctc tgtctataaa ttgattaact atttttatat tattcttatc
    21061 tgcatattca tttattgctc ttaattgagc atctattgat tcgtcccttt gattatctga
    21121 actaaagcgt gcatatacta ctgctttctt tcctgtcata ttatttcctc caaagtttaa
    21181 atatactaat tttaatctat attaaaggct acacactaac actaagtgtg taaccatatt
    21241 ttaatctatc tatcttcttt tacaagtata gaaagtatgt ctactaagct tctacttata
    21301 ttaactgtgt gtctcttttc atagtcagtt ctttcaacta ccttatagtc tttagaatat
    21361 aaatcttttc ttcctatgct gattttataa gtttctgtca tatttatatc ctccatttat
    21421 catagaatat aatcaagagt acataagcac tcttgattat aagttatatt tgaaagttct
    21481 caattatact tctttctatc tgaagcattc ttggtcttat tgttgtatat cctgttgaat
    21541 cgctagcatc ttttgtatga atagtttttt taggagaatc attgttagaa tcaactttca
    21601 caattccact atctcttaac tgtttcacta gttgtctttg acttattccc aaatgtatac
    21661 ctcttttttt attttcaaat tctataaaag aatatacttt atctgtgtaa aagtaataat
    21721 attccttatc cttatatcca catatatatt ctaatttatc ttctataact tggttctggt
    21781 catttaatgt tgctattctt attgactttg aatttattga ttcctttaat gtatttaaaa
    21841 atatatctat aggtgagttt ctcgatatta attttatctg ctcttctatt aatctttcta
    21901 caactttaat cgctctatcc atgtactcat catcttcata cttatctata acacctttat
    21961 ctctaatata atttaacata caactaaaac ctactaatat ccatgcacta gactcaatag
    22021 ttcttccatg tatatcatct ttatattgtt ctctaatttc atccctgtaa gatttaaagt
    22081 tatataataa tatattagat aattcatcaa tatttcccat ttcagataat aaccattcta
    22141 tatatcctct cattgcttct ccaagcaaat gactattatt ctgtaattca gttagtatat
    22201 ttaaatctac tgaacttcta tttaattcta ttgataataa tctagcatta gatgattctc
    22261 cacatatcac atcttcacct gttattaata tcattcctct aggtctctta gcatccttta
    22321 tatctaactt attacttgag cgtcctttcc ctactctatc tccatacatt cttataatgg
    22381 attctgcctt tgagtcttga atttttttat ccctaaagtc ttgtttaggt gcaaagtcat
    22441 cacataagta taagctatcc tttaacttat tagcttttaa ttcaatagct gtaatcgtat
    22501 cattaaatgt agctggaatt tttttattaa aatcaccaaa atgacttaat acaacttttg
    22561 atactgctgt ttttcttgat cctgtttttc catatgtcca tactatattt tccggtggtt
    22621 taatttttct actgataatt tcaataagtg gagataggta aactaatgaa gagaatataa
    22681 tacctttatc atcttctatt aagtctacta gctttaaact catttcacat gctttattta
    22741 tgtcactaat tttatctggt agtatatagt tatctattga ttcatctaac tcaacttgaa
    22801 catctgaacc tcctataact cctcctccat gtagatatat ccacttattg tcttgatata
    22861 tccatcctgt atggtcatat atatacttag tttttatatt catacataag tgttgtatca
    22921 attcaataaa gtcattaata tattttttat ctatagaata tttaaaaccc caattttcaa
    22981 tcacccatgt agttctattt atggtattcg aatctaatat tacctctgat aatagctctc
    23041 cattaatccc catagcattt atacataaat acatttttcc atctataacc ctttgttcta
    23101 ttatatttac taaaaaatta catatctttt tattagatga attcataacg gtgttattat
    23161 ctaaaacatt taatcctatt tcctcatata atttaattaa ttcttctctg tttattgcct
    23221 tgctcatttg tattcctcct atttatccca tttaacaata ttcggtacta aagatatacc
    23281 tctatattta acatttgtat ctttaagtaa gttttcaatt ttcaagtgta atctacttaa
    23341 tcgttgtaag tttttttcgt atgatcctcc tgtattcata agtatgtaaa tagcccttgc
    23401 gttatcttca tactctgtac tacctaatat agttaaacta tattcttttt catttaatac
    23461 accttcatca atcatacctt ctaattcatt acgatttcct tcttcacatt caataagtag
    23521 aattaattct acctttacac ttttaaagtt tgtcattaat atacctcctg ttgtcaaagc
    23581 ccgaaaggcc gtactttttc atcattaagc tattgttctt agattttatt aattagctcg
    23641 ttcctacaaa tatgattata taataaataa tgactactca tatcacctaa agataaacgt
    23701 tttcaccaaa tattttttat ttttttcaaa aaaaatagag aaaatctaat tagattttct
    23761 cttcttctta attctatcta caattttctt cttactttct atatttattg cttttataac
    23821 ctctccatac tttcttacat catctatatt ataatctact tttattttat atttattttc
    23881 aaatgacttt cttccattat ttatattata aatagattta aactcctcta attccttttc
    23941 actaaaattt ttataatagt tgttcttact aactatattt aataactcca aacattctat
    24001 atttataatt ctcattataa gactaacttc tttagtaaat ttttctatat ccttaccttc
    24061 attagttgac aatatattaa ataacttatc gcttagtttc tctcttatcg ttaaaactgg
    24121 aactatgtgt atataagcaa tagtataaga aataaatttt acttgatttt ctatgtcttt
    24181 aattttattt aagtttttaa gagtacactt tcttaattca ttcgctaact ttaattctaa
    24241 gctataaaaa taaatatttt tatattcttc attactattt ataatctcat tatttaaata
    24301 ttcatatata tttttataat tcttatctgt cattttttca agcatattag attttatttt
    24361 cttatgtgcc ttatttgcat catgagtcag tttagtttca tactcccaca ttttagtcaa
    24421 tgatttaagt tctaatttac attttctact aatttttctt cctttgtaat gttttacttt
    24481 attattcctg attcctgcta attttaatat ttctttatcc tgtgtagaat ttgagagatc
    24541 cctacaaact ttatcataaa acattttaaa gttttgatct tctcctatcc attcacttat
    24601 attttccata tcatcatctg atatgtcgtc ttcaaacctt tctttatcaa aaatatctat
    24661 agccatctgc tttaaatttg aattacctag ctcatttttc tttgtttttg ctactatata
    24721 aaatttcaaa aatgatttaa tctcatcaaa atattcttcg tctattactc ctagatttat
    24781 atattttcta tactcaacat gtttcaaaaa tttaattcta atatctatta gactatataa
    24841 tgttttagca atagtatctt catcttcttc gtattcaaat aaactatcat ctaaatctaa
    24901 ataccagtat ttatctttat ctatatatgt gttatcagta ttctcttcaa ttatatgtaa
    24961 tttaaattct tgagtatcta aagtattgtc ataatcatct acatataagt aatctctctc
    25021 tctattttgt aatccttcat cttctaaaaa cttatcaaat gttattttac ttaaatctat
    25081 atcttttttg tttatattgc ctttcatatt tataaagtcc ttt
//
LOCUS       DAMJWS010000062        25009 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMJWS010000062 DAMJWS010000000
VERSION     DAMJWS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.062"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(48..656)
                     /locus_tag="PC399_002532"
     CDS             complement(48..656)
                     /locus_tag="PC399_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF2593385.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(765..3503)
                     /locus_tag="PC399_002533"
     CDS             complement(765..3503)
                     /locus_tag="PC399_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2593386.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINVCISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3651..4442)
                     /locus_tag="PC399_002534"
     CDS             complement(3651..4442)
                     /locus_tag="PC399_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2593387.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4478..5134)
                     /locus_tag="PC399_002535"
     CDS             complement(4478..5134)
                     /locus_tag="PC399_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593388.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5127..6092)
                     /locus_tag="PC399_002536"
     CDS             complement(5127..6092)
                     /locus_tag="PC399_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF2593389.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKNLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6198..6304)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6594..7538)
                     /locus_tag="PC399_002537"
     CDS             complement(6594..7538)
                     /locus_tag="PC399_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2593390.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSASIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLVKSKVLELYNHHCI"
     gene            7649..8116
                     /locus_tag="PC399_002538"
     CDS             7649..8116
                     /locus_tag="PC399_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593391.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8286..8885)
                     /locus_tag="PC399_002539"
     CDS             complement(8286..8885)
                     /locus_tag="PC399_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593392.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            9131..9727
                     /locus_tag="PC399_002540"
     CDS             9131..9727
                     /locus_tag="PC399_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF2593393.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(9825..10784)
                     /locus_tag="PC399_002541"
     CDS             complement(9825..10784)
                     /locus_tag="PC399_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2593394.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10818..11549)
                     /locus_tag="PC399_002542"
     CDS             complement(10818..11549)
                     /locus_tag="PC399_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593395.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(11542..12312)
                     /locus_tag="PC399_002543"
     CDS             complement(11542..12312)
                     /locus_tag="PC399_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593396.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(12397..13107)
                     /locus_tag="PC399_002544"
     CDS             complement(12397..13107)
                     /locus_tag="PC399_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2593397.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(13287..13439)
                     /locus_tag="PC399_002545"
     CDS             complement(13287..13439)
                     /locus_tag="PC399_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF2593398.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(13465..15621)
                     /gene="feoB"
                     /locus_tag="PC399_002546"
     CDS             complement(13465..15621)
                     /gene="feoB"
                     /locus_tag="PC399_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2593399.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(15721..15942)
                     /locus_tag="PC399_002547"
     CDS             complement(15721..15942)
                     /locus_tag="PC399_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2593400.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(15955..16167)
                     /locus_tag="PC399_002548"
     CDS             complement(15955..16167)
                     /locus_tag="PC399_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2593401.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(16488..19385)
                     /locus_tag="PC399_002549"
     CDS             complement(16488..19385)
                     /locus_tag="PC399_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2593402.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLNSGYEYSVLYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKYKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSNLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKANMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLKKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(19712..20320)
                     /locus_tag="PC399_002550"
     CDS             complement(19712..20320)
                     /locus_tag="PC399_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF2593403.1"
                     /translation="MVDNLIKYIHKSYVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(20580..21125)
                     /locus_tag="PC399_002551"
     CDS             complement(20580..21125)
                     /locus_tag="PC399_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2593404.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(21243..23066)
                     /locus_tag="PC399_002552"
     CDS             complement(21243..23066)
                     /locus_tag="PC399_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593405.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(23079..24854)
                     /locus_tag="PC399_002553"
     CDS             complement(23079..24854)
                     /locus_tag="PC399_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593406.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         8246 a         4325 c         2437 g        10001 t
ORIGIN      
        1 cctataaaat accttcctat agatacatat cttacctata taaaaactta ttctatataa
       61 aaacgccata aaaaatcttt tgcttcttct gcataatcta tatttatacg tttactagtt
      121 tttatatcta actcatcttt actaaatact ttttttcctt tatcatcata ataaaaatca
      181 cttatatata attcttcacc cattatactc ttactattta gacttctatc tatttttaaa
      241 gctttgcaaa gttttcctgg tccgttagta atatttttta cttgatattt actcaactca
      301 gtgtaagctt tgttgtatct attcatagat atctcatcta aagaagttat aggctctacc
      361 cctcttatta atacacactc tggcacattt tctatatttg cagataaatt caagcaatta
      421 tacataccat aaattaaata aacatatata tgtccaccat ctaaatataa tggcttagtt
      481 ctatctgtct ttttattgcc atatacatgt gctcctttat catttacacc catgtatgct
      541 tctgtctcta tgattttggt aacaattact ttattttcat atttccttat tagatattta
      601 cctaatattg atttggctaa atcaattcca ttttttctaa aaaaatcctt ttccaaaatt
      661 tcacctcata aaatttattt aaaacgcatc aaatacaact aaagtaataa ataaaattaa
      721 ctcttattaa tgtgatatat ataaacaata aatttagaca tttattattt atatatatca
      781 aaaaattcta tctcaaactt ttcaatatta tcattgtaat ttgaattatc atatacttct
      841 tcaacaacct ctttaattgg gaatataact tttacattag ttccttctcc taccttacta
      901 tctatgtaaa tcttcccatt ttgaatttca atcaatgatt ttgtaaggca taaacctata
      961 ccacttccaa attcatttga tataccactg ttttcaattt gtttaaatcg attaaatata
     1021 tcatttaatt tttctttgtc tatgcccact ccagtatctt ttataattat ctctactgtt
     1081 tcgtcttttt tatatatacc tacaaggatt tctcctcctt ctaaagtaaa ttttatagca
     1141 tttgataata tatttagtat tattctttct atcgattctg ggtcaagccc taccataaca
     1201 gtttcttcat ctgtatcaaa aattaaatct attcctttag acttagcata actactaata
     1261 gatgtaacta ttctttctgt tatgtcaata atatcaaaat taactgcttt aatcttatat
     1321 atgtctgact taacttgaga aatatttatt atattatcta ttaatctcaa taatctaaac
     1381 atattttgtt tagttatttt tatatgttct tttagtatat cctgaaaatc aattattttt
     1441 ccttttttat aaaggttttc aataagttga actgaactat atatattatt taaaggtgtc
     1501 ttaagttcat gagatatatt agcataaaac tccatcttca tttggtcata ctgtctttgt
     1561 tttattaatg cttttttaag tctttcagac tctctttgca cacttaaatc tttaataatt
     1621 ctcactctac ttcttttctg tccaaagtat aatgttcttg atatcatttt tacttcttta
     1681 atgtgattat tttcatttac tatatggata ctcttctctc tttctcctat atttaaattg
     1741 tttatgtatt tttttactaa tatatttcta tatttttgtg ttgtaactcc taatacatca
     1801 ctagaaattc cgaacaactc tctaaaggct ctatttgtat atagtactct tgatgtactt
     1861 aaatcctcaa gaaaaatagc ctctggtata acatccagta atttttcata tattattttt
     1921 tgttcattta ctgttctaag aatatttatc tctttagtta tatctctaaa tgtaagcaat
     1981 ttaaagagtt tattatctga ccaatagtct ccaactctaa cttcttcaac atgctcttca
     2041 ccttctgagt cagacatcac aaaattaact atacagtttg ctgtgtcatt gtaacttact
     2101 atttcagctt tcttttcttg tgttagattt aatcttttaa attccctatt gtcaaagaaa
     2161 tctatgttaa aagtttcaaa atattgttct aatgtcatat cgactaaatc acttgaaaca
     2221 catttaaaac tcttttcata ttttttattt atacttatta ccttattctt ctttatgcca
     2281 ccttcagcac ttcttacaag tataggagat ggaatattta gtataatgtt ttcatagtct
     2341 ttatttatat ttcttatttc ttcatctatt ttgatttcat ttgtaatatc atttaaactt
     2401 attaagtaat tgctttcatc tttattatta cttaaactta acaaataaat tttatcattt
     2461 aactctacca gtttatcaat tatacttata tcgttatcaa atttcttcaa tttttcagca
     2521 actttttcat cttcctttat aaaagttaat attttataat aattattcat atcctttgaa
     2581 aattcatctt taaatttatt atttgtgtaa cttatattaa aatcatagtc tgttactatc
     2641 catacatttg gtattgtatc cagagcttgt ctaagtaatt cttttctctg gtctatgtag
     2701 ttttttaatg ttctttgtat aatcatatcc ttttctattg ctaaatttat ctttcctaat
     2761 tgctctgact ttttacttaa ttttttattg attgaatcaa gttcataata tggatacact
     2821 actctattat taagactgta tacatataca taataagtat ggttacataa tagtaaatat
     2881 attactccta ttaatctatc tgaatctact atattaatct tagaaattgc tacaaaacat
     2941 ataatagttg taaaaaaaac aaaatatgtt aaatctttta ttaacttata gtgctcaatg
     3001 ttactatcaa gtttattcaa caaatatagt aaattaaaca tagatataca aacattaatt
     3061 aatataataa tcatattcca gttaatatca ctaaaaatat ttcttataaa gtaatctaat
     3121 atcacaaaag cactataaaa aattagtatt gggcttattc ttagtttttt tactttagaa
     3181 atggttaagt atataaaagt agacatatta attatgctta agttaagcaa tacaatttgg
     3241 aatttttgaa tttttaaatt tactactcct aaaatactta caaaaactaa tacaagacac
     3301 cctactttca atatatttat aaattcatct gttttaaagc tacttatata aactatagaa
     3361 tatccaaata tccaaagtaa aattattcta tacatattta tagaaactgt atacaaattt
     3421 atatttaaaa gtgcaataac caaaaataac ccattagcta gtatatagac cttattctta
     3481 cttaaagttc ctttagattc catattcccc accacttctt catttttata tagttttacc
     3541 tataaatatg ttaccacaat tcttccaaaa aatttgtcac tttctagtta taaaaaaaaa
     3601 tgagtaaata tttttaattt tactcatttt gtagattttg tatattcagt ttaaaaagct
     3661 ggaactatac ttcctttata ttcatcttgt ataaattttt taacttcttt actattcata
     3721 gcctctgata aagccttaat cttatcacta tctttattat tttctctaca agctattata
     3781 tttacatatg gagaatcact tgattctatt actattgcat ccttagtagg atttaagtta
     3841 gctgttaaag cataattaga atttattact gctccgtcaa catcttttaa tactgttggt
     3901 aattgctctg cattcatttc ttttattact atatttttag gatttttagt tatatctttt
     3961 ttagttataa gctctccatc ttttacttct atcaatttat tttttgctaa taatttaagt
     4021 gctctagctc cattagttgc atcatttggc acagctataa cagctccatc ttttaattca
     4081 tctaatgact taactttttc tgaatagaat cccattggct ctatatgaac tttagatgta
     4141 taagtaagtt tatatccttt ctccttcaca gtttcctcta aaaatggaat atgttgaaag
     4201 aagttagcat ccaaagaccc ttcatctaaa gctgtatttg gaagtacata atcatcaaat
     4261 atttttactt ctaaatcata tcctttttct tttaacaaag gttttgcaac ttccaatatc
     4321 tttgcatgtg gatttgatga agctcctact agtatttttt tatcctcttt gtttgaacaa
     4381 cctactgctg atattgctat tgctgacacc aatgctactg ataataattt ctttaatttc
     4441 atttttactc cccctcttta aaatgtttat aattaattta ttttagtttc ttataagcca
     4501 gatttcctac tccctggatt atctgaacta atattataag tgctattaca gtatatacca
     4561 ttactgctgt atcaaatctt tgatatccat aaataagtgc tatattacct aatcctccag
     4621 ctcctactgc acctgccata gctgtataac caagtataga tattattgat aatgtaattc
     4681 cagatactat tgaaggcata gcttccttta tcataacttt aaatactatt tgtctttttg
     4741 tagctccaaa agattttgct gcttctatca atcctttatc aacttcattt aatgagcttt
     4801 ctataattct agcgataaac ggtgctgccc caatagttat tggcactatt gcagcagttt
     4861 cccctatact tgttccaacg attaatctag ttattggaat cattgcaact ataagtatca
     4921 taaatgggaa acttctaaat atatttacta taaatcctaa tgcactattt attgtgctat
     4981 ttggttttaa tccatctggt tttgtaacta ctaatatgat tgctaatata aatcctaata
     5041 ttgttgccac tatagttggt actataacca tatacaaagt ttgtaacaat gcatttggaa
     5101 atagtgttgt taaaaagtca attaaactat tcatttatca tttcctccca tcttatgccc
     5161 ttactagtta gatattgttt tacttgctct ccacttttat ctgaaatgtt tataataagt
     5221 gaacctaaaa tatcatcttt aaattgttct aatttcccaa atattataga cacatctata
     5281 tttagttctc ttgccatagt cgtaataata gcttcattag aaatatcttt agggaatagt
     5341 atctttatat ttgttccttt tggcaatatt atactttctt cacctattaa ttttctaagt
     5401 cctttagtat ttcttaagaa tatttcttct gtatctccta cctctaatac ttctccattt
     5461 tccattattg ctactcttcc acaaatctgc tttataactt ccatttgatg agttacaact
     5521 attattgtta ttcccaaatt tttatttata tcttcaagca atgaaagaat tgactttgta
     5581 gtatttgggt ctaatgctga agttgcttca tcacaaagaa gtacttgtgg atttagtgct
     5641 aaagctctag caattgcaac cctttgtttt tgtccgccac ttagatttct gggtttatca
     5701 tttttctttt cagatattcc aactacttct aataactcta aaactctttt tttaatttct
     5761 gcctttgagt atccaaaaca ttctaatggc aatgctacat tatcaaatac agtttttctt
     5821 tcaagcaatg aaaagtgttg aaaaatcatt cctaactctt ttcttaaatc tctcatttgc
     5881 ttttcattca atgactttac ttctttatca gaaacaagta cactgccttc ttggtattct
     5941 tctaatccgt ttatacatct cagtagtgtt gatttccctg caccactatg acctattatt
     6001 ccaaaaattt ctccagattc tatttcaata cttacatctt taagcacttg aatctttcca
     6061 taatacttat taacattttt tatgctaatc ataaaatttc ctcccatata ttctgctcag
     6121 tttttatgtt tatcctctct tatgtattac aacaaaataa aagcccgagt ataaactcag
     6181 gcttagaaag ctatttatac tcatctatca actatattag ttgcaggatt tagcaccaat
     6241 gtatataaaa tatactggtt gccgggtttc aaagggccag tccctccacc actcttgata
     6301 agacgttatt taatttaatt ctattatcat ttgttgctat gttatatatg atatattcat
     6361 taatagtatt tgtcaataaa tttatttaaa ttttttattt tttattatcg ttaattaact
     6421 atttttaatt agttttctag ctcctaacac tttatatttt aatgtctata taactataga
     6481 acatcattat aagaaataaa ttaaataaca tcaatctatc taaatcatct gtaaagtaaa
     6541 ttaagaaaat taacatttta tccaaactct atcctactat taaataaaat aatttaaata
     6601 caatgatgat tatatagctc tagtacttta gactttacca aatgtttaag ttctataggt
     6661 tctattattg tagcattttt cccaaaagtt aaaaaatatt cagccaccca gttaagtgta
     6721 gattttggta tatacatatc tatgtatcca cctttagaca agattttcaa ccctcttgcc
     6781 aacaaaaatt ctgtttcaca acgttttact ccctcatctg tcaaattaat tttaatatga
     6841 taatctgtac ctacttccat attcttaaga taatcatgta tagaagataa aaccttatat
     6901 ttcttacttg ataagttttc tatactcaaa atcttcacaa ttctgtctac acgaaattcc
     6961 ttaatagatg ctgattttat acagtaagct ggacaatacc aaagtccatt catggcatac
     7021 aaaccaatag gaacaatggt acgtacacta tttttttgct ttgatgaata ttcaatttta
     7081 attgtagatt cattcattac tatattaaac aattctttca ataatggtgt tgaacagtgc
     7141 ctatctggtg tccaaaaaac aaatttacgc cttatattct caatattata ttgaatatca
     7201 tttggtatac aatttaaaaa tttttttaaa acagaaatag tttcttgctc aaatggcaaa
     7261 tcattataat attctagtga ctgatatgca aagaatatag attttacctc actttctgaa
     7321 aatgcaatcg gtggaagtat acgctcttta agtacatgat atccaccagc cgctccaact
     7381 tttgaatata atggaaatcc aatactctcc agcacctgta agtctcttaa tatagtacgt
     7441 tttgatacag aaaattcttc tgctaattct cctgctgtaa aacttctttt tgcatttatt
     7501 gttatcatca gttcaattaa acgctcagct cttgacatat ttatactcct aatttaaata
     7561 tagtgacaat aattgtcact attatatgat aagatattct tacatttaaa gcaataataa
     7621 tagtaaagaa agaagggact gatactttat ggaaaataat actattaagg ctgatgatat
     7681 tatcaattat tgtctatcaa acttagatga tgttttatta atgaatagtt ggggggaacg
     7741 agcaatttac tacaatccta atggtatttt aaagcgagga gtatatgttc ttaccattaa
     7801 agaaaaagac agtaatcatg ataaaggttc gttggttagt cgcccaaatg tataccgtgt
     7861 gaatatagga ttaaaaaaag aaacttttat tgaaatgttt ggatatattc caaaacgtcc
     7921 aggtgtaggt caaatagttg atatggattt tgattttaca aaattggaca caatcatgcc
     7981 acatcctatc tactcatgga tgggatggat atgcgtccta agccctactg aaaagacatt
     8041 tgagaacttt aaaactttaa taggagaatc ttacaatttt gcaaaacaaa aatttaaaaa
     8101 gagaaaaaac aagtaaaaac gtatgaaaat aatccctaaa taaattataa taaaaatatt
     8161 aaggttacaa gataaaaatt aatatcttaa taacatgcaa aaaagcgaag ccaaaaaatg
     8221 tattatttat aaggcttcgc tatatctcaa ataatttatt tcaaatttca aaactattta
     8281 ttctattatt tcttattatt tcctgttatt cctaatctat cattcatatc ttttatcatt
     8341 tttctaaaac cactacctat atcatttcca acatcttcag ttccatgaac taattttata
     8401 aagtcatttt gacctttttt catatcttca tcagatacct tatctaattt ttctattctc
     8461 ttatctatgt cttttactaa tctttcccct tcagcgatta ggtcatcatt cattttcttt
     8521 aactctgtat cttctgtttt accatctttt ttaagagatg ttacaaatga ttgtatttta
     8581 tctctactat ctttatatgc agcctttaca tcacttagat atttttcatt tcctggatat
     8641 tctttatctt taaactgatt atttatactt tctggagtat cataaataga atatctattt
     8701 agacctgcta tataatcatt atagcttgtt gaataataat tcatatatcc ttcattatta
     8761 tttacagatt cattaggatt ttttcctgta ttttcacttg gtttttcatt atttttagta
     8821 catgcagtta tacctatagc taatactagt gctaatccta caaataaagt tctttttaat
     8881 ttcattttta ttaccctcct agatgtttta tactataaaa aattttaata attttctttc
     8941 ttaatttagt atttctatat ttttatattt tatttactaa aattaaacaa gttataattt
     9001 atttttttgg aaatcataat gaataaataa ttaaataaaa aaattataat tttattattg
     9061 atattgatta tcattcatag tataatctaa ttacttgata ttaattttca tttgaaggag
     9121 gttatctttt atgtcttgtt gtaattcaca attttgttgt aaaaataaaa ttgaatcatc
     9181 atatataaaa tggcttttag aagttttagg tccagtcttt ttaggctcta aacctgctga
     9241 aataataaat atttcgttta ctgatgtaaa tagagaggaa aaaattaatg atatttataa
     9301 atatctatat aaatgtaaga aaatagattt tatagttatt gataaagaaa aaaagggatt
     9361 aaaaatctta tttgtaaata aaaaagcatt atcagaaaaa ttaaaatgta aaaaaacagt
     9421 aaacttttta aagtttttag gatacaaaca aaatacgaat gttaatgctt atttagagca
     9481 tttagtagat aaacttaaga gtgatgtttt tcctgatgaa ataggtattt ttttaggata
     9541 tcctcttaaa gatgtgattg gatttatggg atatagtaat tatgaagttt ctatgattaa
     9601 atattggaaa gtttatggtg acacaaaaca atccgaggac acatattcga agtttttact
     9661 tcaccgtaag aaaatgagaa aattactaga ctacataagt gtagataaaa tagtaagctg
     9721 tttttaattg tatttttgta taataataaa aatacaccct cttttaccat atagtttcca
     9781 taataaataa gggtgtattt tccactttaa tattttaaag tattctaatt ttgattgtac
     9841 aacatatctt taatatttat tttcttttgt tgcattccat tagacattaa aaattcctgt
     9901 gtatcttcta attcttttat atccttgtcc gttattttag aactaaagtc ataccaagaa
     9961 tacatttctt ttgtttcatc taaagataaa ccaacttctt tagatacaac atccatcact
    10021 tcatccttgt tttcattcat atattttaaa gtttcttcat gaaccttcat aaatcttttt
    10081 actaattcag gatatttttc agcaaagtca tcacttacag cagtaactat tattccactt
    10141 actaatcctt ctccatttac aatcattttg gcacctgaat ttatcgtttt caatgccact
    10201 ggacctgcaa gcattgctac atcagcactt ttactttcta atgcagaagc agcactagga
    10261 atatccatat ttacatattc aacatcatct atagtatagc cttctttagc aagtgcagca
    10321 atcaacagtt gatgaagtat agtccctttt ggacctacaa tttttttacc ttttaagtct
    10381 tttatagatt taatattatc attattagtt ataagcatat atccttttgg tgaacggcta
    10441 taaatattcg taattttaag accaacacca tttgatgcag ctattattgc agacgttcct
    10501 cctaatgcat gtaaaaagtc tagctcacct gctgctaatg cttgagtttg ctctggtcct
    10561 gttgtcaatt catggaaatt gacttttata ttgtcctttt taaattcctt tccaaataaa
    10621 tcatcctttt tttctaatat actaggaaca ttaagtggtg attttacata agttaaattt
    10681 atttccttag gtaaactagc ctcttttttt gcaggttcat ctttcttatc atttgttgtt
    10741 gaacatccta cagtcccaac caacaccatt attaaagatg tcattactgc taaaatcttt
    10801 ttgtttcttt taatcaatta aaactccccc aattccctta atatttcttt tttcaagttt
    10861 atataatagt ttttagttaa atctctatta tattcatctt cgacactaaa ttgtttaatc
    10921 cttttatttt tactaaaaac aattattttc tttgctattt ccatagcttc ttcaatattg
    10981 tgagttacaa atactacacc ttttttagta tttttatgga tatttacaac ttctttttgc
    11041 atttttcttc ttgtgaagta atctagtgct gcaaatggtt cgtccatcag caaaatatcg
    11101 ggattgaagg aaagtgctct tgctatactt actctgtgag ccattcctcc tgataattca
    11161 tttggatatg agtttttaaa cttttcaagc ttcatcattt ttaaatactt gtctaaatca
    11221 actttatttc tattatcttt ttcagtatgt aaaagtatat tttcacttac attcagccat
    11281 ggcataagtc tactttcttg aaataccatt cctacttttg gagtacactt tttatcatta
    11341 ttaaaaaaat atatgtttcc actagtagca ttttcaagcc ctgctaaaat tctaagtagc
    11401 gttgttttcc cacatccact ttcacctaat ataacagtta tctcttcact agatatattt
    11461 aaagatatat tatcaagaac taaatgctct ttattatcaa ctaaatattt cttatatata
    11521 ttctctagca aaaatccact tctaaccatt taaatcaacc tgcttccctt tgctaaattt
    11581 tgttattatc tttgaaaata gataatctga aagtatacct aaaaatccaa taacaaaaat
    11641 tcctaccaat acaacatcag ttctagacat atcttttcca tcagaaatca aatatcctag
    11701 tcctgaagaa gctgcaataa gctctgctcc aattatagcc ctaaaactat acccaagccc
    11761 taaactaagt ccaacaacaa tatccagcat agaattaggg attattattt tataaaaaat
    11821 tctcttttta gataaattaa aagatttacc tacttctatt aatttattgt cacaattttt
    11881 tatacctttc aatgtattta aaaatattgg aaaaaaggat gccaaaataa taattataat
    11941 tttagattct tcacctatac caaaccataa aataagcatt ggcaccaaag ctagtggagg
    12001 tgtacttcgc ataaattcaa atagtggttt gaaatattca tacatttttt tatttactcc
    12061 aaataaaact ccaagtggta tagccatcaa tgaacttatt aaaaaaccta ttaatatcct
    12121 tcttacactg gcataaacat ttaagaaaat agaaccgtca tatatcattt ttataaaagt
    12181 atctaagact atttcaggag gaggcagaat atagctactc catataccca aaacacatac
    12241 tatcttccat aaaattaaaa ttattaaaaa aatataatat cctttaagcc ttttatgaat
    12301 tttctcaaac atataaaact ccttttaaca tatctatccc tgctaccctt tttatttttt
    12361 aatatcttaa ataataatta agctatatca taagatttaa tctatgccaa gtcttaatcc
    12421 acctgaactc attctatcag aaggtgtaga atctgttttt aagccagaca atctttcaga
    12481 tggttgtata atgactattt gtccaggttc aacaaattcc atatgataag attctcctga
    12541 agtctgacca ataaaagtct tccatgatag ttttgatact ttaggatatg gttctcttcc
    12601 agtccaagct actaatgcat ctgggtcaac acaacaaggt ccttcaagaa ttagaggatt
    12661 accatctgtt attatagcaa catcttgatt ttgacctgtt ttatttacta aatgtgtagt
    12721 aaaaagacct ttttgagata ttacccctga accaatcaat tttacactgt aatttaattg
    12781 ttcagaaaat gccaataagt tttcactctc aacataaatg gaatcacctg ggtcaattga
    12841 tattacatct acatggtaag cattttgagc taggtaaatt tcaccttcac cttcaaccac
    12901 cattattggc atttgttctc ctgtcaatga cctctgaaca tgacctaata atgctcttcc
    12961 caatccaccg ccattacttg gacctaacag caatttttca aaattaaaat ttcctttaaa
    13021 ggctaccata gcaccttttt ttgcaaaaaa tttaccactt ccccttgcta tacacataag
    13081 ctcgtttgta acatcaaatt taaacataat attcacttat attgcttaat ttagcaataa
    13141 tccctcctaa tcatatattt atatataatt atacagtaaa attacagcat agtctaacct
    13201 acttcaaata tgaaatttgt tatttataca aaaaataaga acctgataat aaagcaattt
    13261 aactttttat caggctctta tcctatttat ctcttagcgc tatgacaact cttactactt
    13321 gagcatccag aacaaccaca tccacaactg ctaccattct tttttgaatt tttaaccata
    13381 tacattacag ctagtgccat taatactact acaatagctg ctataataaa tgtagccata
    13441 ttcatcactc cttatttata tgaattaatt tttcatataa cttaatttac ccattttttc
    13501 ttttttcata tttttatttg aataagttaa aactacaaag actactgcta ctgctataaa
    13561 tatagatatt attgccccag caatacttcc tccagtacct aaaactaaac ttccaacttg
    13621 atttataaga agagcaatta tatacgctgt taaagtttgg aatcctaatg taatccatgt
    13681 ccatttccat gaacccattt ctctttttat ggctccaata gctgcaaaac aaggtgcaca
    13741 aagcatatta aatgccataa atgcaaacgc actagctact gtaaacatgc ttgcaactga
    13801 agcaagaagt gttgggtctt gctctgttgc atctgatata ccaaataaaa ctccaaatgt
    13861 tccaactaca ttttccttag ctactagacc tgtaactgtt gctactgatg attgccaatt
    13921 tccaaatcca agaggagcaa atattggtgc aactatattt cctaagctag ctaatatact
    13981 atctccagca tctaccattt gtaatgacca attaaatgat tgtaagaacc agattactcc
    14041 acaagcaaca aatataattg ttcctgcttt gattataaat gcttttcctc tatcccacat
    14101 atgtattaaa acaccttttg cacttggaat atgatattga ggtaattcca taacaaatgg
    14161 agatggttct cctgcaaata aactagtttt ctttaatata attccacaaa tgattatcat
    14221 tgcaatacct aagaaataca ttgatggagc tacccatgaa gcacctccaa ataatgcccc
    14281 tgcaaataat gctattattg gaatcttagc tccacatgga atgaatgtag ttaacataat
    14341 agtcattttt ctatctctat cattctctat agtacgagtt gacataactc ctggaactcc
    14401 acaacctgaa cttataagca ttggtataaa tgattttcct gaaagaccaa acttacggaa
    14461 aattctatcc attataaaag ctacacgtga catgtaccca caatcttcta atattgataa
    14521 taataagaat aaaagcatga tttgtggtac aaatcctagt actgctccaa ctccaccaat
    14581 tagaccatca actacaagtc cttgtaacca ctctgctaca tttaaagaag ctagaaaatt
    14641 acttacattg ccttgaataa tttctccaaa cagtacatca tttgtccaat cagttgctat
    14701 agtacccaat gaacttactg ctatatagta aactccccac attataagtg caaaaatagg
    14761 taatgctagt atacgatttg ttacaatctt atctatctta tctgatacag tttccttacc
    14821 ttttcgattc ttctttatta tacttgaaat tattgaacta ataaattcat aacgattggc
    14881 tgttattata ctttcactat catcatccaa ttcatcttca caatttctag ttatttcttc
    14941 aatactattc aatattgttt tagaaatatc aagtttttgt ataacatttt catctctctc
    15001 aaatagcttt atagctaacc agcgtgtttc tacatctata tatggagtct tttcttctat
    15061 tattttttca attttttcta ttgcatcctt actttcatca acaaagggta atttgaaatt
    15121 taatttattg ttactacttg caagttcaac tgccttttca gcagcctcca taattccttg
    15181 acctttaact gctgttactt ctaccactgg gcaacctata acttcagata gttctttaat
    15241 atttatctta tcaccattct tatttacaat atccatcata ttaagagcta taacggtcgg
    15301 tatacctagc tctaaaactt gagttgtaag atataaattt ctctctatat ttgatgcatc
    15361 tacaatattt attactgcat ccggcttatc atctagcata aaattacgtg taactacttc
    15421 ttctaatgta tatggagata aagaataaat acctggtaaa tctactattt ccacatcttt
    15481 atttcctttt aattttccac cttttttttc aactgttact cctggccagt taccaacata
    15541 ttgtgatgag cctgtaagcc cattgaacat tgttgtctta ccacagttag gatttcctat
    15601 gagacctatt tttattgaca tttccattcc ctccattatt atttaaatac atcactattc
    15661 atgatataga aaatcattct cattatataa ttacattaac tctactctaa ttaatcttta
    15721 ttattccaca ataatttttt ctgcatctga cttacgaatt gatagttcat aatctctgac
    15781 tgttacctca attgggtcac caagaggagc tacttttctt ataaatatat taacgcctct
    15841 tgtaatcccc atatccatta tacgtcttcg tgtagctcct tctccttcta actttttgac
    15901 ttttacagtc tctccacact taatatcctt cagtctgttc atgtcattac ctccctatat
    15961 tattattcta cttgccattt ttttatctaa tgcaactcta gtatctttta cgtctattat
    16021 taagttacca tctatcttag aaactatttt aacatttgta cctactacaa aacctatact
    16081 atttaaaaaa gtcctcgtct tatcacttcc tgaaattttc tttataacta cttcttcacc
    16141 tgtatttgat agtatcaatg gcatcatata atatccctcc tcttaaacta atttaaatct
    16201 acaagcaatc gttatcttat atataaatta caatacctta aatcttaatc tattaaaata
    16261 atatcctatt ttttccttct ttttacaatt cctaattttg atttaagtct atcacaatag
    16321 tatatttttt gcaagtgatt attgtattct tttgattttt tttctcattt gatttatttt
    16381 attaataaaa tcgtaaaaat taatacaaaa ataaagatgc tctaaaatag tatactcaac
    16441 taatattaaa gcacctttat tatatatatc attacttaca cattgcttta ttctttttta
    16501 aaagcaattt tttcaaactc ttttacaggc attggcttag aaaacaaata tccttgtacc
    16561 atgtcacacc caatttgctt tagaaattct acttgcgaaa tagtctctac accttctgat
    16621 attactttca ttttcaattc ctttgccatt ttaatgacat tagatataac aattttttca
    16681 ttattactct catctttttc tgtaaaaaat gctctatcta gttttaatat gtcaacaggc
    16741 aattctttta aaagattaag agaggaatat cccgtaccaa aatcatccat agagattaca
    16801 aatccctttt ctttcaaatt gttcattgta tcaagtaacc cttctatatt atctaatact
    16861 gctgtttctg ttagctctaa ttctataagt cttgtcggaa ttttgtattt atctacaatt
    16921 aatttaaact tctctataaa attactattt acaaaatgca ttcgagacaa gtttacagat
    16981 ataggtacta ctttttgtcc ttcatccatc cattctctaa tctttttaca cacttcctca
    17041 aatacataga aatcaatatt tacaataaaa ccattcctct caaatacagg aataaattct
    17101 attggtggta ttagaccttt ttgtggatta tcccatcgaa caagagcctc tgcccctata
    17161 atttgataat ctgaaagact atatttaggt tgaaaataaa caataaattc tccattatta
    17221 agagcatcta ccattgaatt ttcaatttct ttctctttta atattttttt atgcatttcc
    17281 ttatcgtaaa aagcaaatga atttttatga ccacctttta tagtttttct tgcagtatta
    17341 gctctatcca taatagtatt tatgtcatta tcacttttac ttattttata tataccacaa
    17401 tctaaaatca atttataata gtttatttta ggattactaa gtatttgaac cttattgtaa
    17461 atattgttta accttgtttt aatatcatca tctgtcttat acttaataag aagaacaaaa
    17521 tggtcagcag aaactcttgc aaaaatttca tcttccttta attcacttgc aatagtattt
    17581 gatatatgta tcaaagtatc atttcccata tcatatccaa acatatcatt tatatattta
    17641 aatttgtcaa catctatata aaacaatgca tattcttctg gattattttt agcaaacagt
    17701 ttattcgaat ttattttaaa tttatctata ctatctgctc ctgtcaatgg gtctgtatat
    17761 gctatcttct ttagataatc ctctttcttc tttttattat aaattatata tttaatagca
    17821 attaagaata aaatacatac aaaagaaata atttgtattg aatattcttt aaaaatcaca
    17881 ccaaatggta ctttataagg tttgtcaata gtattagaat atatacaatc acttaattct
    17941 tcttcagtaa ttttgtctat tgatttattt aaaatagata ctaatatagg gtcagtttta
    18001 tttgataccc ctatacgcat cccatagctt gtatctaaaa cagatataac agataaattc
    18061 tcactctttc cttctcttaa aagttcatca aactgataag aactaagtat cattaagttt
    18121 gctttacctt catttatagc gtttagacaa ctttctatac tcttataaga tgttgtctta
    18181 gcattttcaa aacttttttc tatataagaa tcgatagact tataactact cgttaaagca
    18241 atatttaaat cattattgag attggtatcc agttttccta ccataatcat tggaatattt
    18301 atgtatggat ttgtcaattt catattaaat cgttttgcct tatcattttc attgattgag
    18361 ccacatagca tatttgcctt accacttttt atgtaatcaa gactctcatt ataatttttt
    18421 gttttaatat attcaaattt tataccacat ttttttgata taattgacat taaattggaa
    18481 cttatacctt taaacgaatt gctttcctta tcataatact ctacaggaga ccactctgaa
    18541 tcatatacca tagtaagttt tgggtttgct ttaataaaat tcatttcttg ccgagtaaat
    18601 gctatggaat tattgacctc tcttttgaaa tattttttat ataattccaa ctcataatac
    18661 atatcattta tttttaattc ttttaaagca taatctaatt cttttaccaa attaggttta
    18721 tattttgcag tagcaaaata aaaaggctct actgaaaatt tgctaactat tttcattcct
    18781 ttagcattta taatacttcc acagacaaat gcatctgcct ttttttcatt gacagactca
    18841 attaattcat caatagaatt acagtagata tattcaactg aaaaattatt ttcttctcta
    18901 tacttctcaa atgcaatatt atgaaagttt cctctaagta cagctactct cattccctta
    18961 aaggaactta tatcttcata aaacatatta ttgttactat cttttgtata tagtacacta
    19021 tattcatatc ctgaatttag tttaggaaaa ttaaatagtt tatttctttc atcattcttc
    19081 tgtaaaggtc caagtaagtc tatttcacct ttttctagtc tgtctaaaca ttcttcccaa
    19141 gaccctttta caaattcata ttcccatctt gtatattttg agatttctct tagataatca
    19201 taaccatatc ctgtataaga atgctctttt gatgaggata ttattccgta gtcatcacaa
    19261 tatccaactc ttattttttt tgaattttca gactcatcag caaagcatag tatattgtta
    19321 aaacatgata taataagtaa aaatatcaaa gaaagtatca agaagtgctt tttaaatgct
    19381 ttcatattta aaaacctcca atttgccaac ttactttaac ttatttggtt ttctagttgt
    19441 aactactttt attgtaatgg taaatatatc aaattgcaat attctcatat aatattctta
    19501 ttcaactata atgaatttat tattatctat ttaaatacac tatctaatat tagtttctta
    19561 taagtcataa aagcacaact aacactataa ttctagtgat taattttatt tcaaaaaata
    19621 taataacact actaatattt taaatatcaa tagtgtcata aaaacttttc agattaatta
    19681 aatctttcta tatattttat aataatctaa atcatgctaa atatttagac agaaaacttt
    19741 tttctttaat agtaaatact tttgtttgaa tatcagaata attatttgaa tatctatcta
    19801 taaaaccatg atttgcatct aaaatctcaa tttggatatt ttgtttccta gaaagttggt
    19861 aagaaataga atcaacatca aatgaatcgt gctttgcaaa tactaataag gtaggacatt
    19921 ttggaattac atccatgtaa tctcgaatcc gagagccata acaacaaatt atgccatcac
    19981 aaaaagaatc ttcactgcat ctccatgcaa tagttgctcc cacactatac ccagcaacaa
    20041 atacatattt atactctgat tttaaatcat ttataagctt ttcaactttt ttataaatat
    20101 caaaaccaac attatttata aaaaaatcat atgcctcttg gacttttgaa tatggaaaaa
    20161 ctcttttttc tcccagaagt tcaggacaaa atacatcata tccatattta taatactttt
    20221 gacaaacatc ttctataaag ctattaatcc catagatttc atgtaaaaca acaatagcta
    20281 cataactttt atgtatatac tttatcagat tatccaccac ctttacaaaa cagcaaattt
    20341 tttatactta gtttaatata ttcttcttta catgtcaatt tacttttcta aaatcaaatt
    20401 tatttttgga ttaatcattt atttttaaaa gttgtgcatt attactatta aattattctc
    20461 tattattaaa aatataatcg ttagaaataa aaaataaagt acaaataatt aaaaaatatc
    20521 tcactcataa ctttaaaaat tgtaagtgag atactcctaa aatctacttt atattaaatt
    20581 tattttccaa aatatctatt taataatcct aagaatgctt taccatgtct agcttcatct
    20641 ttacacattt catgtactgt atcatgtata gcatctaatc ctaattcttt tgctttctta
    20701 gctaatttta attttccatc agttgcacca tactcagcat caactctaac tcttaagttt
    20761 tcttttgtat ctgcaactac aacttctcca agaagttctg caaatttagc agcatgttca
    20821 gcttcttcaa gagctattct cttataagct tctcctactt ctggataacc ttctctatca
    20881 gcttgtctac tcattgctaa atacattcct acttctgtac actcaccaac aaagttagct
    20941 cttaatcctt cgattatttc ttcatctaca ccttgagcta ctcctattct gtgctcatca
    21001 gcccaatttt tttcgccttt catttcttca aatttatcag ctccaacttt acatactgga
    21061 cattgtgctg gtgcagcatc tccttcatgt atatatccac atactgtaca aacaaatttc
    21121 ttcataatta ctttcctcct aaattttata tatcaaatta aattttttat ttattaagtt
    21181 agtctatata tacccgcacc atataccttt aaacaatttt tttaaaagtt ttttctaaat
    21241 acttattttc cctgtgtcaa taatatgttt ctataataat gtccctttaa ttttatcaat
    21301 tctttatgat ttcctgattc cacaattcta ccattttcta aaactattat attatcacaa
    21361 tttgatatgg tacttaatct atgagctata attatacttg tctttccata agttaaatta
    21421 tttattgctt cttgaatttt agcttctgtt gctatatcta tgttacttgt agcctcatcc
    21481 aaaataagta ttggagcatt ttttaatact gctctagcaa tacttattaa ttgtttttga
    21541 ccttcactta aatcttcacc cctatccgat aatacagtac tatatccttc acgcatttta
    21601 actataaatt tatgtgcatt tgctatttta ctagcatata taacttcttc atcagtagcc
    21661 tccagatttc cataccttat attttccata actgtatcat tgaatatata accttcttgt
    21721 ggaactactc caaaacaagt tctcaaatag tttctgttaa tagagtatat atccttacca
    21781 tctaaaaata tacttccttc atctatatga taaaaatttg taattagatt tataatagtt
    21841 gtttttccac ttcccgtctc tcctatgatg gcatttgatg tgccagattt gatttttagg
    21901 tttatattat ctaaaatttt cttttttgaa tcataagaaa aacttacatc tttaagctca
    21961 atttctccct ttacaaaatg agagtttaaa ttaccatctt tattatcgtt aatctcactt
    22021 ttaaacatgt tatgcacttc ttgctcttct tgttcatcta taatttcaaa aactctttca
    22081 catccagcta tggctgaaag taatgtatta aatagatttg ctaattcatt taaaggtctt
    22141 gtgaattgtc ttacataact taaaaaacta gctataaccc ctatggttat aatatcttta
    22201 gatgcaagaa tgcctcctat aatacatata gttgcaaatc ctatattact aatcacattc
    22261 ataataggca ttaaaagaga agtgtaaatt tgcgatttaa gggaaatatt tagaagtcta
    22321 tcattgactt ctttaaaatc atttacagat tttctttcat aattaaaaga ttttactaca
    22381 tttatgtttg atatcatttc ttcaatatgt gcatttagtt ttcccaactc aacttgttgt
    22441 tgcttaaaaa aaactctagt tctctttgca ataaaattac tcacaaacat tactatcggt
    22501 attaatatta cactgcaaaa ggttaaaatt gggcttaata caaacatcat aataaaagtc
    22561 atcaatatgt ttaagattcc tgtaattaac tgtactaatg aatttgatat tcctgtactg
    22621 atattatcga catcatttgt cactctactc ataatatctc cagtagaact tttatcaaaa
    22681 aaggttattg gtagattttt aaattttaaa aataaattac ttctcatttc tctgactata
    22741 ctttgagata atctagctgt acatattccc ttagacagtt taactgaaaa ctcaattata
    22801 taaaatgaaa gtaaaataat tattcctttt aagaaaatgg attttgaata agtatcgata
    22861 tctattatgt caactacctt tccaatagta tatggtatta cagttgatat aattgaatca
    22921 attattacta gcaaaaaaat aaatctagta atcttcttat cttttttaaa gtacataaaa
    22981 attcttttaa acgttttttt aaaatcttta ggcttttctt ttttctttaa tctactgacc
    23041 ttctgatttc cagtacttaa attcaatata cttcctcctt aatttacatc ttcacttact
    23101 aaattttgca acgcaaaaat ttttttataa attggagaac taaccagtaa atcttcatgt
    23161 gtagcaaatc caaccacctc accctcatcc aagactaaaa tcttatcaca gtcaattata
    23221 gaactaatct tttgtgaaat agttattaca gtacatttct tactttcctt ttctcttaaa
    23281 ttacttatat actcttttat attctttctt ataattgtct ctgtattaat atccattgca
    23341 ctaaaacaat catcaagaat taatatttgt ggttttttgg ctatagctct agctatagta
    23401 attctttgtc tctgtcctcc tgataaattt ttaccccttt ggaataaaac ttcatcataa
    23461 gtattttcga gttttgttat aaattgattt gcatttgcta tttcacatga ttttacaaca
    23521 tcctctttgc atatatctct atttcccata actacattct ccaatatact ttttccaaac
    23581 acactactat tttgaaatac tacacctatt cttgccctta aactttcttc agaaatactg
    23641 tatatactac gtcctccaat cttaatatca cctttatcaa ttatatataa tttttgaatt
    23701 aaatttacta aagaagtctt tcctgaacca gtttgaccaa tcacaccaat attttgacct
    23761 tcttctattt taaaggatac gttttttaat attttttcat ctgtattttt gttatacgaa
    23821 aaatctacat tttcaaaaat tatatcacag tttacaaact ttaaatcttt ttctccaaca
    23881 tctaaacttt cttctctctc aattacttct tcattctcat caaccaaaac ctcacttatt
    23941 ctctcataag atgcttttgc cctaacaaaa acattaaaaa catgtgaaat cattataaga
    24001 gatgttaata ttctagtcat atagtttaca taagcaacta taatcccaat tttaatttct
    24061 cctgaataaa ttaaatgcct tccaatcagt aatattatta ttatggaaac attaatgatt
    24121 attgtaattg tcggtctaaa aaatgacatc atagttgtag cttttctact atttttaaaa
    24181 agttcatcat ttaactcttc aaagctttgg ttctcataat cgtatctaca aaatagttta
    24241 actactttta ttccacttaa aaactgtctt aattttgaat ttactttatc tgtactttct
    24301 tgtacatttt taaataatct ataccctatc ttcatattaa gcaatattat aataaatata
    24361 attaaaactg caattagaat aattattgac atctttaaat ttaattttat agctataatt
    24421 aaacttccta tacacattat tggtgacttt ataaaaactc tcatcattcc attaacgaat
    24481 tgttgcattt gattgatatc attagtaatt cttgttatta atgaagctct accaaattta
    24541 tctatttgtt ttaatgagta tgtatttatc ttttcaaata catcctgtct taaatcataa
    24601 gaaaaatgct gagaaactct tgccgagatt acacttctta ttattgcaaa tattgcacct
    24661 aaaaaagtta tacctatcat tatagctcct gttgatagta tgtagtctaa gttcttattt
    24721 gaaaccccta tatctattat ttttgccatt atagttggtt gagctaaatc acaaaaagct
    24781 tccaatgtta aaaaaaatac tgctgttaga aataatacta tatatttttt tatgtatttt
    24841 ttaaaatacc tcattcaatc atcccttcat agaattttct cattaccaaa agtatatcat
    24901 aatggctaat ttaattgatt atatcaatta taaatatttg atttattatt aatttttata
    24961 ccatatattt tataaattat ttgtttttat ttcctatatt tatatatat
//
LOCUS       DAMJWS010000063        24627 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMJWS010000063 DAMJWS010000000
VERSION     DAMJWS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.063"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     regulatory      95..185
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            594..911
                     /gene="flgB"
                     /locus_tag="PC399_002554"
     CDS             594..911
                     /gene="flgB"
                     /locus_tag="PC399_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF2593407.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            919..1323
                     /gene="flgC"
                     /locus_tag="PC399_002555"
     CDS             919..1323
                     /gene="flgC"
                     /locus_tag="PC399_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF2593408.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1333..1650
                     /gene="fliE"
                     /locus_tag="PC399_002556"
     CDS             1333..1650
                     /gene="fliE"
                     /locus_tag="PC399_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF2593409.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1675..3225
                     /gene="fliF"
                     /locus_tag="PC399_002557"
     CDS             1675..3225
                     /gene="fliF"
                     /locus_tag="PC399_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF2593410.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3238..4308
                     /gene="fliG"
                     /locus_tag="PC399_002558"
     CDS             3238..4308
                     /gene="fliG"
                     /locus_tag="PC399_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF2593411.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4301..5032
                     /locus_tag="PC399_002559"
     CDS             4301..5032
                     /locus_tag="PC399_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF2593412.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5036..6355
                     /gene="fliI"
                     /locus_tag="PC399_002560"
     CDS             5036..6355
                     /gene="fliI"
                     /locus_tag="PC399_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF2593413.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            6386..6832
                     /gene="fliJ"
                     /locus_tag="PC399_002561"
     CDS             6386..6832
                     /gene="fliJ"
                     /locus_tag="PC399_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF2593414.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6861..8066
                     /locus_tag="PC399_002562"
     CDS             6861..8066
                     /locus_tag="PC399_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF2593415.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            8084..8692
                     /locus_tag="PC399_002563"
     CDS             8084..8692
                     /locus_tag="PC399_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF2593416.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8719..9702
                     /locus_tag="PC399_002564"
     CDS             8719..9702
                     /locus_tag="PC399_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF2593417.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9715..9918
                     /locus_tag="PC399_002565"
     CDS             9715..9918
                     /locus_tag="PC399_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF2593418.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9918..10736
                     /locus_tag="PC399_002566"
     CDS             9918..10736
                     /locus_tag="PC399_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF2593419.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10726..11421
                     /locus_tag="PC399_002567"
     CDS             10726..11421
                     /locus_tag="PC399_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2593420.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11433..11876
                     /locus_tag="PC399_002568"
     CDS             11433..11876
                     /locus_tag="PC399_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF2593421.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIADLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11891..12265
                     /locus_tag="PC399_002569"
     CDS             11891..12265
                     /locus_tag="PC399_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HDF2593422.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12266..12931
                     /gene="fliP"
                     /locus_tag="PC399_002570"
     CDS             12266..12931
                     /gene="fliP"
                     /locus_tag="PC399_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF2593423.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12944..13213
                     /gene="fliQ"
                     /locus_tag="PC399_002571"
     CDS             12944..13213
                     /gene="fliQ"
                     /locus_tag="PC399_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF2593424.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            13225..15039
                     /locus_tag="PC399_002572"
     CDS             13225..15039
                     /locus_tag="PC399_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF2593425.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNNIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15048..17123
                     /gene="flhA"
                     /locus_tag="PC399_002573"
     CDS             15048..17123
                     /gene="flhA"
                     /locus_tag="PC399_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF2593426.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGDLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17098..18114
                     /gene="flhF"
                     /locus_tag="PC399_002574"
     CDS             17098..18114
                     /gene="flhF"
                     /locus_tag="PC399_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF2593427.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18111..18989
                     /locus_tag="PC399_002575"
     CDS             18111..18989
                     /locus_tag="PC399_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF2593428.1"
                     /translation="MIDQAQILRKIAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19003..19704
                     /locus_tag="PC399_002576"
     CDS             19003..19704
                     /locus_tag="PC399_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF2593429.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19720..20142
                     /locus_tag="PC399_002577"
     CDS             19720..20142
                     /locus_tag="PC399_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2593430.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20127..20312
                     /locus_tag="PC399_002578"
     CDS             20127..20312
                     /locus_tag="PC399_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2593431.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20353..21126
                     /gene="flgG"
                     /locus_tag="PC399_002579"
     CDS             20353..21126
                     /gene="flgG"
                     /locus_tag="PC399_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2593432.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21147..21908
                     /gene="flgG"
                     /locus_tag="PC399_002580"
     CDS             21147..21908
                     /gene="flgG"
                     /locus_tag="PC399_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2593433.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21931..22812
                     /locus_tag="PC399_002581"
     CDS             21931..22812
                     /locus_tag="PC399_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2593434.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22815..23189
                     /locus_tag="PC399_002582"
     CDS             22815..23189
                     /locus_tag="PC399_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2593435.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23221..24624
                     /locus_tag="PC399_002583"
     CDS             23221..24624
                     /locus_tag="PC399_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2593436.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
BASE COUNT        10186 a         2633 c         4611 g         7197 t
ORIGIN      
        1 gtaattattg gtaattaaat ttaaaaaata aaatatttaa ttatacacaa aaaagatatt
       61 taaaaaattt gttatttatt gtatacttaa gttaaaacta aataggcaaa tctagagaaa
      121 tctagtgacg caaagctata gggactaagg tttatataca taaactatgt cagccagttg
      181 ccaaaaagag tcctaggtgt attgtatacc taagaaaagt ctataatgac tgcctttttg
      241 gcagtcattt tgttatggaa aaatcacttt atttcagtag aaaatacaca taataacata
      301 ttttaccaat tataacataa aataaagaaa aattatctca aaaagttact attttacaaa
      361 aaagagcaac ttttcgaaga aatatttaaa tacatattaa aattttttta tattttcatg
      421 ggacttcatt aaaaaaatat tagttttctt actaaattga tacattattg tattaatgta
      481 atggaaatgc gattaattta aataaataaa gaagtcattt tttgtaatat agcaacttca
      541 ttttttttat taataaacaa aatagataaa atatattttg aggaggggtg agtgtgaata
      601 catatgacat tataaaaatg ggattagatg caactaatct aagaagtcag acaatagcaa
      661 ataatatagc aaatataaat actgaaggat ataaaagaca ttatataact tttgaagaaa
      721 cactaaatga tgctatgcct aaaatagaag aaaaaacaga caattctaaa ggtttaagag
      781 tagatggtaa taatgtagac atagaagaag aaaaagtaaa tcaagcaatg gcaacattac
      841 aatataatgc tttgataagt tttgcaaatg gaaaaatagc aatgaccaaa tcaataataa
      901 gtgggaggta attagaagat gagtgtgttc agtggaatga gaatatctgc tagtggatta
      961 acagcagaaa gtttgaaaat ggatatcata tcatccaatg tagctaatat aaatgctaca
     1021 agaggtccaa atggagaagc atataggaga aaagttgttt catttgaaga aaactatgat
     1081 aataaattag gtatgctagg agtaaaagtt acatctgttg aagatgataa atcacctctt
     1141 aaagaagtat atgacccatc tcatccagat gcaaagccaa atggatatgt agaatatcca
     1201 aatgtaaata tattggtaga aatgacagat ttaataacag cttcacgagc atatgattct
     1261 aacgtagata cattgaatgc tcagaagtct atgatatcaa aagctttgga aataggaaag
     1321 tagggataaa ttatgccaat tacacaaatt aacaatttaa tgcaaggtgc aaagttagat
     1381 aatttgattg gagaagcaaa gccattaact cttaataata aagaagaaaa aaagaatttt
     1441 gaagatgtaa taatagatac tatagataaa gttaattttc aacaagtaac agcagatgat
     1501 atgaaagaaa aatttataaa aggcgaagat gtttctatgc atgaagttat gttaataggt
     1561 caagaagcac aactatctat gcaatattta atagaagtta gaaataagat tcatgatgca
     1621 tatcaagaaa tgaataaggt gcaaatttag ccttttgaac aggagtaata atcaatgata
     1681 accagtttta aaaataagat taaggagcaa gttgctaaat ctaggacaaa gataagttct
     1741 ttaaaaaagg gaactaaaat agcattaggt ataggggtta ttgctatatg tttagcgcta
     1801 gtatttacag caatatctgc gagcaaaaat aagtatgcag tcttattttc agaactagat
     1861 gctcaagatg ctgcagatat atcagcagag ctagagtcta aaaagataga tactaaggta
     1921 aaaggaagta cgatttatgt acctagaaca gaagttgata agttaaggct ggagttagcg
     1981 ccaagtctta caggtggttc taaaggattt gcactaatgg atgatagttc atcaataggg
     2041 cttactgatg aagaatttca gattaagaaa caaagaatga tacaaggtga aatagaaaaa
     2101 acaataaaga gttttccaca agtggaagac actagagtac atataacatt tggagagtca
     2161 tcagtatttg agaaggaggc tgttccaggg agtgcagcag tttatttgat gctaaaacca
     2221 gggacgactt tagaaaaaaa ccaaataaag tctgttatgg cacttatatc aggaagcgtt
     2281 acaaatatac cagagaaaaa tgttcaggtt attgataaca aaatgaattt attatctgaa
     2341 ggtattttgg aggatgaaaa tgttgatgaa aataatcaac caataaatag tggcattagt
     2401 gttgataaaa ataaggcatc tgaacaagca ttgaataaac aactagaaaa gtctatttta
     2461 gaaatgctag aaccgatatt tggtaaggga aaggtgaaag ctacagtaaa tgctgattta
     2521 agttttgata cagctgaaaa aactgagata aaaattgacc ctgataaagt tgcaataaag
     2581 gagacaaggt cgaaaaattc atctaaatct gctgcaaata atgttcaagg tgtagatgct
     2641 aacatgaata accaaggaaa taacaatggt actaatacag aagatagttt agatgagagt
     2701 tttgagtatg aaacaggtaa gattgaaact cacactatag ttgcgcctgg agaacttaat
     2761 agattgacag cttctgttgc aatagatgga aaaatagcta aaggtgttca agctgatgta
     2821 gaggatattg ttaataatgc tataggtatg gatggagcga ggggagatac tcttagcgtt
     2881 gtttcaatgg tatttgaccc agaaggtaag aaagaggcta aacaagagct ggaagagatt
     2941 aaaaaagaag gatttaaaaa gaacataata aaagcagtta ttggagtagt agcagtaata
     3001 gcagttggag ctattggata cttaatatat aagaaaagaa gtgaacgaga agatatggat
     3061 tatgaagatg aagattatat ttctaaatct gattctgttg ataggaagga taatcctcta
     3121 ttaggagatg atccagaact gacattagag gaagctgtta aaatgtatgc agaagaaaag
     3181 ccagaccaag taacagagat aataaagact tggttaaatg agtaaggggg atagaaagtg
     3241 tccacggaaa ttacaaatgt taatgcgttt gacttaagtg atataaatga aagtgataag
     3301 aatgagaagg tagaaggtga gggtttaaaa aaagctgctg tattattaat gacattaggc
     3361 ccaaatgtgt ctagtgatat aataaaaagc ttgcctgata aacaagttca aaaaataggt
     3421 ttggaaattg caaatatttc ttcaatagct tcaaaagaaa gaagagaaat cttaaaagag
     3481 tttgtagaaa taaatagggc aaaagattat gttatagaag gtgggcttga atatgcaaga
     3541 gagttattaa gtaatgcttt aggaaatgtt aaagcaaaca aattacttga aggtatatca
     3601 ataaatacaa gtacaaagcc ttttaaacat attataaaac tagatgtaaa gaagatatta
     3661 gaaatactta ggaatgaaag tgcacaaact atagccgttt tacttgcaca tatggaacca
     3721 gaaaatggag cagaagtttt atcatactta gattttgatt tacaaaatga agtagctatg
     3781 agaataagct caatgggttc aatatctcca gaagtaataa aggtaattga aagttcatta
     3841 gaagataaat tacattcaat aagtggtgat gattttaatg attatggagg ggtgtctacc
     3901 ttagttcaaa tattgattaa ttctgataga aaaacagaaa agaatatttt gggaactatt
     3961 gaagaacaaa acccggattt agcagctcaa attaaatctc atatatttgt ctttgaagat
     4021 attgtcaagt tagatgatat aagcatacaa aaagtgttaa aagaagtgac tatgaaagat
     4081 ttagtatatg ccttaaaagg tgtagatgat gagatttcag atgctatata tagaaatcaa
     4141 tcttctagag cagcagatgc acttaaagaa gaaatggaaa tggttggtgt tataaaggtt
     4201 acacaagttg aggcttctca acaaaagata gctaatatcg taagaaaact tgaaagagaa
     4261 ggtgccataa ctattgtaag gaattttggt ggtgactttg ttgaataata agattattaa
     4321 aagtaaaaaa gcattatata aaggagtgct agatttaacc ataatagata aagttagagt
     4381 gggagaatca aatgtaaata aagaaaatga ttgtagattt agggatgagg taataaaaga
     4441 agctatagaa tctgctgaga taaaaaagaa tgagatatta gaagaagcaa aaataaatgc
     4501 acaaaatata gaaaagcaag catatgaaaa agggtatagt caaggtcaaa aaaatggata
     4561 tgaggatgga tataaagagt catatgatga atatacccaa aaagctaaag atgaagcaga
     4621 ttttataaaa aatcaggcaa atttaatctt attacaagct aatgagaaga tgactgagta
     4681 cataaaagaa aaaaataaag agattgtaag acttgctatt aatatggcca gttctgtttt
     4741 aaaaaagcat ttcgaagatg aagaatctat gagtgatctg ttaaagcaaa ttattcaaga
     4801 ttatgaagga agtagttcat ttatagtaag atgtaattct ttctataaag ataatataga
     4861 gaaagagttt gaattattca aagaggaaac atcactaaaa tcaagtgtat ttgttattgc
     4921 agatgatagt atagataaag gcaatgctat tatccaaaag gaaaatggaa gaataatagt
     4981 tggtattgat tgtgctatgg aaaaactaaa agaagaattg cttggtgaat aaagtatgta
     5041 taaaactgat atatctatag attttgacaa aataaataag aaaatagaaa atttagaact
     5101 cactatatca gaaggtagag taaagaagat aataggactt actgtagaag ttgaaggaat
     5161 aaaagctttt gttggggagt tatgcgttat atacaaccaa gttaataaac cagtgaattg
     5221 tgaagtagta ggttttaaag ataaagaagt catattaatg gcgttgggag aattaacatt
     5281 gatagcacct ggttgtaaag ttatttctaa ggggatacct ttaagtgtta tgtgtagtga
     5341 taatttacta ggtaaagtac ttgatggtct tggaaatcct atagacaact cagatgctgt
     5401 tttaggagat agatacaacc tgaacaatga acctcctgat ccaatgaaga gaaaaaagat
     5461 acgaaatata atggaaacag gtgtaagagc aatagatgca tttactactt gtggtgaagg
     5521 acagagaatc ggcatatttg caggaagtgg agtaggtaag agtacgactc taggtatgat
     5581 tgctaggaat gcaaaagcag atgtaaatgt aattgctctt attggagaaa gaggaagaga
     5641 agtactagat tttattgata aagatttagg tgaagaggga atgaaaaaat ctgttgttgt
     5701 gtgtgcaaca tcagataaag cccctctagt taggttaaaa ggtgctctta ctgctactgc
     5761 aatagcagaa tattttagag accaaggcaa aaaagtaata cttatgatgg attcagtaac
     5821 tagatttgct atggcacaaa gagaagttgg tgtagcaata ggagagccac cagctcaaaa
     5881 aggatataca ccatctgtat ttgcaatgtt gccaaaactt atggagagaa cagggacatc
     5941 agataaaggt tctataactg cattttatac agtattagtt gatggagatg actttaatga
     6001 gccaatagca gatacaacaa gagggatact tgatggtcat atagttttat ctagagactt
     6061 ggcaaataag aatcactatc cttctataga tgtattaaac agtttaagta ggcttatgaa
     6121 tgaaattgct tcaaaagaag acataaaaat agcgtctttt gcaagagaca tgcttgctga
     6181 gtatagagaa gcagaagatt tgataaatat aggtgcatat gcttcaggta caaataaaaa
     6241 aatagatgaa gcaatctatt atcatgagca tattattaat tttttaaaac aagggataaa
     6301 tgaaaagagt tcttttaatg aaactataag tagtttgaga agagtttttg aataaacaat
     6361 taaaaaaata tttgaatagg ttgtgatgga attggataaa aactttaagt ttagattaca
     6421 gaaagtttta gacttaaaag tgaaagatga agaagagata aaaatggaat ttgctaagat
     6481 tcaacaaaaa aaaatagata tagagactaa tttagaaaac ttagaaagta attatagcaa
     6541 atattctata agtaaaaata acgatagtat tcaaaatcaa aaaataacta ttaactattt
     6601 actagcttta aacaattcta taatggattt atcagaagag ttggataagt caacaaatga
     6661 acttgaaaaa gctagaaagc agcttataag caaacagata gagagaaaat cattagaaaa
     6721 attaaaagaa aaaaagtatg gacagtatta taaagaagaa aatttaaaag aacaaaacac
     6781 taatgatgag tttgcaagta tgagttattt aaggaataga caagttctat aagaatagta
     6841 ggaagtgagg atattattgt atgaagatac aagcagattc ttataatatt attaaagata
     6901 ttggggatag aaaaagttca tctaagaaca aaaatataaa aaataataaa tttgaagata
     6961 atctatataa ggcaacagac cataaaaaag ttaaagatag aagggaagta agtaagcaaa
     7021 atattagctc agataaaccg gaaaatagtg ataatgagtg tcatttagat gaagataaaa
     7081 gtaatcactt agaagatgaa aatacatatg aaaaagattg tagtaagaat aatgaagctt
     7141 tgcagcaatt actgaatctt ttaaaggata attataaaaa agataaagat ataaacacta
     7201 tattgaataa tatagaaaaa ctaagtatat cagatgaatt aaaacaagaa cttaagctta
     7261 ataaaaatat attaaataag attgcaaatc aaaattcaga gctaaataaa gctaatataa
     7321 ataactctaa tgatatagaa aatttagaca tagttaaatt aagagaactt ttggaagata
     7381 atgctaataa agatattgaa gtaattacta aaactacaga aaatgatacc aaaaatatag
     7441 atttgtatag ttttgatagc aatagaatgg ataatcttaa ctcaaataaa cagaaagatg
     7501 atagctctga tattttggaa aaactagcag gggtaaatgg aaataagagt gggaattttg
     7561 gacaagtaat aaataaaaat gttgatatga ataaaattaa aaatgataat gtcaatttaa
     7621 aacagataga atcaaatgta atggatgact ctataaaagc tattaagcat atgaaaacaa
     7681 atgatataca agaattgaca attaaattaa gaccaaaaga attaggagat atgaatatac
     7741 aattgttaaa agatggtgaa agtatgagag ctgttgtgac tgttttcaat aaagatgtct
     7801 ttgactctat aaataaaaac atagcagatt taaagcaaca tcttgaactt actaatgtaa
     7861 atataaaaga tgtatcagtg caaatgcaca gcgataatac aaatacatct gatacctttg
     7921 ataaagcatt tgaacaacag aatagacaaa ataaccaaga aaacatgagt aagaagaata
     7981 caaatgataa gcataataat gtagtcatag aagatgacat taaagaaaat actattgatg
     8041 atgatagagt agatttacta gcttagtaag ggggaagtat tgcatgtacg gaatagcaac
     8101 ggctttggca ggaactcagg gaaactatag gtcaaataac ttaagccaac ttgaaagaaa
     8161 tattaatgat attgccaata aacaagttag aagcaaagga aatcagacag taacagacaa
     8221 tgggactcca attataggac aaggtgatga tgctgataaa gacttgttta tgaagatact
     8281 tgtggcacag atgggtaatc aagacccaat gaatccgcaa gacccaacag aatatgtgtc
     8341 acaattagca cagtttgcaa ctttagaaaa gatgtcagct atgaatgaga agctagatgt
     8401 acttttagcc ttaagtaatt ccactctaat aaactctgct ttaagtatgg caacatcatt
     8461 aattggtaaa gaagtagaag tatctgggga agagaaagaa gatgaaaaca ttgtaggagt
     8521 agtaaagtcc acatatataa aagatgggga agtacactta gaattagaag tagatggaag
     8581 tgacaaaact gtaggtgtta aatatgaaaa attacttaaa ataggagagg tagatgaaaa
     8641 ttcaggagaa gaaaatgatt cttcagaaca cgaatctcca gaagaagcat aatattaaat
     8701 gagaaaagag gtaaattcat gataaaagca atgtattcag gtgtaagtgg aatgaaagca
     8761 aatcaaacaa aattagatgt aataggtaac aatgtagcca acgctggaac tacttctttt
     8821 aagaaaagtt cagctagaat aacagattca ttttatcaaa ctgtactata tgcaagtgct
     8881 ccaacagctg cattaggagg aacaaactta ggacaagtag gtgtaggtag taagatatca
     8941 agtataaata aagatatggt acaaggaaat gtacaaccaa ctaatagacc atctgattta
     9001 atgatagatg gagatggata tcttccagta gtaagacaag gaacagttat gtatacgaga
     9061 gatggttctt ttaatttaga tacaggattt aaagcagctg atggtgcttt gaagaatatt
     9121 aaaggtggaa gacttgtaaa tggtgatgga tatatgttac aaggtatgat ttatgatggg
     9181 gaatataaag ctgatactgg agaatttgtt aaaaagggtg atgctaaagg aaaagagcct
     9241 attgtaatac cacttcaaag tataagtcct aaggatggaa cagttcaaaa tgtactagag
     9301 tataatatat caaaggatgg aacagtagaa tttttattga gtgatggtca aagaactcaa
     9361 tttgtctatg atatacctga tggtgctaag gtaggagata cacctgctgg aatagataaa
     9421 ttaaaaggta caatacaaaa agtgcaaaca tatgcattcc aaaatccagc aggactagat
     9481 gcagaaggag gaaacttgtt taaaccatca gcaaactcag gagacccaca aatagcagga
     9541 gctacaggca caataataca aggggcaata gaagcttcca atgtagatat agcagaagaa
     9601 tttacagaaa tgataatagc aagtaggtct ttccaagcaa acagtaaaat aatatcgaca
     9661 tcagacgaga tacttcaaga aataataaat cttaagagat agtaggtaca tattatgata
     9721 agagtaacgg atttaaacgg aagaaaatac atattaaatt cagaccttat agtgaggatt
     9781 gattctgtac cagaaagtag gattctcctt acaacaggag aaaaaaacct tgtaaaagag
     9841 tcggtagatg agatagtaga taaagtaata aagtataaaa gaaaaataaa tgtaggttat
     9901 attaggagcg aaaaataatg aaaaaaacag attcactgac atttattgga ctaatagtat
     9961 ctacagtatt agttttagtt ggtgccgcaa aaggaagtag ttcagggtta aagaattttt
    10021 ttgatgtttc atcaatatta atcacagtgc ttggttcatt tggagctcta atgattacat
    10081 ttactataga tgatataaaa cttataaaaa atgcattaca atactcattt aagacaatga
    10141 gtgtatcaaa gttagattta ctagaacaat ttaaaacact ttctaaaaaa gctagaaaag
    10201 aaggtctttt atcaatagag agtgatgtaa tgggaataga agatccattt atgaagaaag
    10261 ggttagagct gtgtattgat ggattggaaa tcaatgagat aaggtccatc ttagaactag
    10321 agatagattc tgttgaagat acaaataaca gatactctaa actatttaaa acttggggaa
    10381 cctttgcacc tgcttttggg atgttgggaa cactaatagg tttaatacaa atgttagctg
    10441 accttacatc tccagattta atagcatctg gcatgggaaa ggcattaatt acaacgtttt
    10501 atggttcttt acttgccaat atcgctttga acccaattgc atataatata gatgagaaga
    10561 ctgaaaaaga aatttatgta aaggaaatga tgttggaagg tataataagc atacaatctg
    10621 gggagagcag tatagtagtt gaggaacgtt tagccacata cctttcaaac aatgagaagt
    10681 tagaaattat gaaatccaat aaaaatacag aaagagcaat gagcaatggc gcgtaaaaaa
    10741 aataagaaaa aggatgagat aaatccagat gcatggctgg ctacttatgc agatactata
    10801 acactgattc ttacattttt cgtattattg tatgcaacat cagttgtaga tatggaaaaa
    10861 tttaaaaaca tagcagatgc gttaaaaggt cagttttctg ggacaagtat atttagtacg
    10921 ggttcaagta caagtgagaa atctccaata gacagtgttt tagtggataa aaatgataaa
    10981 tcaaatttag aacaggattt aaaagataaa gttaataatt ctaacttaca aaatagtgta
    11041 acagtcaaag atgatagtag agggatttta ctagagttag atgattcaat tttatttgac
    11101 tctgggattt ctgagttaaa agtagatagt aaagctactc ttaataaagt atatgacatg
    11161 ataaagatga tgaaaaataa gatagttata gaaggccata ctgataatgt acctgtaaaa
    11221 tcaagtacac atgaatccaa ttgggattta tcttctagta gagcagtaag tgttgtaaga
    11281 tactttgtag aagaaaaggg catggaccct agactatttt cggcaacagg atgtggagag
    11341 tatcaacctt tggtttcaaa tgaaacagca aagggaaggg ctgagaatag aagagttaat
    11401 atacttataa ctattgacta ggaggaagag gtttgttcaa aaataaaaaa attattataa
    11461 ttattttgat actgattatt ttctcagcgg gagcaggttt ttttatatta ggagaaggtg
    11521 gaaataaaca agttgacaag attgcagatt tgtttattcc aacaggaaaa ggtaaagatg
    11581 aagtaaagac cgaaaatctt gacttagaag agatggtatt aaagctagct gatgaaggtg
    11641 taagatattt aaaagtatca ataactgtat cttatgattc ttcatataaa gatatagaaa
    11701 aaaatacgcc tttaattagg gataatatta taaattgttt tatggcaaaa agggcagatg
    11761 attttactgc tgataattta aagaacataa aagaagaaat aaaaaataca ttaaatatac
    11821 aactaggtga aaatgtaatt caaaatatat atttcacaaa tatagtagtg caataaaagg
    11881 atggtaagac atgatacttt atttactaaa aatattaata tgtatcccaa taattttaat
    11941 attgatagtc ttatctttaa aaattagcca aggaacttta ttaaaaaaat ataatgataa
    12001 atatgtaaaa atactagata ttgtaaatat aagtaaaaac aatagcataa taattttaaa
    12061 aataggtgaa gaaggatgca ttatggcatc ttcttcttct agcatgagta aaataaaaga
    12121 tttgaccaaa gaagaaatat taaaaataga aaacgacatt aaagaaagaa attttaaaat
    12181 tcaagatttt aaaatggaaa gaattaaaaa gaaatatgat ttcagtagat atattggaaa
    12241 atttacaaaa aaggaagata agtaaatgga actattatca aaattacctt tggctgatgt
    12301 accatataca agttcaatgc aattgttttt atttctaact gcattaatgt ttttaccatt
    12361 tgtaatgttc atgatgacaa gctttgtcag aatagttata agtttatcat ttttgaaatc
    12421 agcattagga gcacagcaag caattccaag tcaaattttg gtaggacttg caatagtatt
    12481 gacaatattt ataatgagac cagtattaaa tgagataaac gaaaaggcat tacaaccata
    12541 tatgaaagaa gaagttacta tggaagaagc tatgaaggaa gcagaaggac ctattaaaga
    12601 gtttttatta acacaaacca gacagacaga tttagattta tttgtagaac aggctggttt
    12661 aaaagaaaag aaattaactc gtgaaaatat accactatct gttgtagttc cagcatttgc
    12721 tataagcgaa ctaaaaactg cttttcaaat aggtttttta atatatatac catttttgat
    12781 aatagacctt gttgtggcaa gtgttttgat gtctatgggt atgtttatgt tgcctccagt
    12841 catgatatca ttaccattta agttattatt atttgtaatg gtggatggat ggaacttgat
    12901 agtaaaaaca ttgatattgg gatttggata ggagaaaaat agtatgagtg aatttatgat
    12961 aatgagtgtt cttaaaaatg gcatgtttac aggagctatg attgcagctc cagtactcat
    13021 tgtttctctt gtagttgggc ttttaataag tgtgtttcaa gctacaactc aaatccagga
    13081 gcaaactttg acttttgtac caaaactaat agctattctt ttagtagtag ctattggtgg
    13141 aagctggatg ttacatactc ttattagttt tacttctgag atattcaata tgatatctct
    13201 tataactaaa taagtaggat acatatgatt tttgtattta ttagaatagt agcattcttt
    13261 ggaacactaa gaatactatt tccagctggt acacctgctt tatttaagtc actctttgca
    13321 atcattatat caatattgat ttcaagtact atgcaaattg aatatgcaac aaatatagac
    13381 aacattgtat tgtttacctt atatggtgta aatgagacca taactgggat tatgcttgga
    13441 tatataacca acctatgttt ttatagtatc agaatggcgg gttctatgat ggaccaacag
    13501 atgggattat caatgattaa tatgtttgac ccaaattcaa tgactcagac aactttaatt
    13561 gataatctta tgaactggac agcacttatg atatttttta gcatggatgg tcatcatgtt
    13621 ttaattagag gtataaggta tagttttgag ttaattccaa taggaaaacc atttgtagat
    13681 aataacatag attatataat aaacatattt gtgcagtgct ttttaactgg attcaaaata
    13741 gcaataccta ttgtactttg tctgcttatg gcagatttta ttttagggct tatatcaaga
    13801 agtgtacctc aattaaatgt aatgatagtt ggtatgccat taaaaatatt ggttggtatt
    13861 gcattattta tcatatcaat acctttaatt gtcaatcaaa taagtcattt attaagtcaa
    13921 ataccaaaga tgtatgaggg aacttttgca ctagctccta tgttttttat gggttcagca
    13981 gataaaacag aagaagctac tccaaagaaa aagggagaac aaagaaagaa aggtaatata
    14041 gcaaagagta gagaattacc cgttgcaatg actctacttg cattcacctt attagtacct
    14101 acattatttt catatgtagt agatacactt aaatcttcgc ttaattattt tttgagtcta
    14161 gatttttata tgaacataaa ttattcaaac ctagagaaat tagttatagc aggattaatg
    14221 gattttttta agatattttt accaatagct attccctttt tagttttagg cataatagca
    14281 aacctatttc aggtgggaat actgtttact ggtgaaactt taaaaccaaa tttatcaaaa
    14341 ttaaatccaa ttagtggatt caaaaacatg ttttctatga gaagtttgtc aaccttaata
    14401 aaggatacag cgattatatc tattttggct tacataggat atacattctt ccaagataat
    14461 tatttagata tattaaagct aggtaacata tacctaccaa ctttaatgta cacagttaaa
    14521 gacttagtat attcaatatt gagtaagatt tgtgtagcta tgatagctat agcagttgca
    14581 gattatgttt atcaaagata cagtcataaa aagcaactga gaatgacaaa acaagaagtt
    14641 aaagatgaat ataaaaactc agaaggagac ccagaagtaa aagctaagat aaaacaaaaa
    14701 caaagacaaa tttcgtctca aaggacaatg caagcagtac caagtgctac tgtaatagta
    14761 accaatccaa cccacttatc tatagcagtg agatatgaaa aagggaaaga ccaagctcca
    14821 gtagtggtgg caaaaggtgc agattatcta gcttttaaaa taagggaaat tgcaaaggga
    14881 aataacatcc ctataataga aaacaaacct attgctaggt tgttatataa gcaagtagaa
    14941 atagaccaag aaattccaga ggacatgtat caagcttttg cagagatatt agtggcagta
    15001 tataaaatca aaaatagata caaagtcccc aaaaggtagg ataagtaatg aataagttta
    15061 atttaaagga aaatatggat gtaatagtgg catttggtgt tgtagggatt gttcttatga
    15121 tgatagtgcc attacctaca tttatattag atattttact ggcaattaac ataagtttat
    15181 ctgtgctaat tttactaatg acattattta caacaaatgt acttgattta tctatatttc
    15241 caacagtgtt acttgtgaca acactattta gattgggatt aaatctttca tcaactagat
    15301 tgattttaac acagggatat gcaggtgaag ttatagaggc atttggaagt tttgttgtag
    15361 gtggaaatta cgtagtagga ataattatat tcttaattat attaattgtt caatttgttg
    15421 ttataaccaa tggttctgga agagtatctg aagtaacagc tagatttacg ttagatgcca
    15481 tgccaggtaa gcagatgagt atagatgcag atttaaatgc aggagttata gatgacaaaa
    15541 ctgctagaaa aagaagaaaa gagcttcaac aagaagcaga tttttatggt tctatggatg
    15601 gagcaaataa gtttgtaaag ggagatgcca tagcaggtct tatagtaaca gcaataaaca
    15661 taataggtgg aattgtaata ggagctgtaa tgctgaatat gactcttatg ggtgcagcac
    15721 agacatatgt aagacttact ataggggatg gtcttgtaag tcagatacct gctctattaa
    15781 tatctacatc agcaggtata atcgtaacaa gagcttctac tgatgaaaac tttggtgact
    15841 tagtaggtaa acagttaacg gcaataccaa aagcagtaat gatgactggt tgtgttctaa
    15901 tcgtacttgg tatgatgcct ggattaccta aaccagcatt tttttcacta ggtataggag
    15961 ctatagcagc aggttacttt ttaggaagag aaaagaaaga tactttagaa gacttggagt
    16021 ttgaaaaacc tgatatggta aacccagata aaaatgaagt agaagatgta acaaatttaa
    16081 taaatgtaga atcaatagaa gtagaaattg gatatggttt aatatcctta gctgatgaat
    16141 cagcaggagg agatttactt cagagaatag catcaataag aagacaatgt gcaatagata
    16201 tgggtatagt cgtacaaccc ataagaataa gagataactt acagttaaat ccaaaccaat
    16261 acaatataaa aataaaagga aatgtagttg gtgggtatga gataatgcct aatatggttt
    16321 tatgtatgga ccctatgaat caaggattta gtatatttgg aataaagaca attgaaccta
    16381 cttttggaat agaggcactt tggatagaaa gtgacaaggt agaagatgca gagttaaagg
    16441 gttatacagt tgtagattca tccacaatac ttataactca cttactagat ataataaaat
    16501 caaatgcaca cgaattactg ggaagacaag aagttaagac tattatagat gcagcaagag
    16561 aaaattatag tgctgtagta gatgaattga taccagattt gatgacactt ggtgaaattc
    16621 aaaaagtact taaaaatctg ttaagagaaa aagtaaatat aaaggataga gttacaatac
    16681 ttgaaacgct tgcagaccag tctagaaata caaaagatat agagcttctt actgaatatg
    16741 taagaatagc cttggctaga agtatttgta ataatttagt tgatgatgat aagacaattg
    16801 tagtggctac attgtctcca gaaactgaag agttggtatc taataattta cagaggtctg
    16861 taaatggaac ttatccagca gttgacccag aaaatacaaa taaaatattt gaaagtatac
    16921 aagaagtaat gagcaatgta tatttcaatc ataatatacc agtaatagta gtatcaccaa
    16981 agataagagc tccatttaga aaattagttg aaattgtata tcctaatttg actgtattat
    17041 cattaaatga aatacctaac gatattaaaa tcaaagcaga aggtgtggtg agcatatatg
    17101 atgacgaaaa agtatacagc taacacaatt caggatgcta tgaatctagc caaaatggaa
    17161 ttgggtgaca atattacatt aatagataaa aaagaggtaa gaaagtctgg aatacaaggt
    17221 atcttttcaa aaaaagatat tgaactaact attggatggg aaaagagaga aaatgtagaa
    17281 caaaaagatt taaaacgaga gatagaacaa ttaaagtcaa ttattaacaa tatggggttt
    17341 gataataaga atgataatga tatagataaa atatgtaaaa atctactaag tttagaattg
    17401 aatgaagaaa ttgtagaatc tataagaatt gatttgcaag aaatgaagtt caatggaatc
    17461 gatacaagta aaaatttagt agaaatttta aagaaaaaga ttaaaataga aaatcaagta
    17521 ataaatggta aaattgctct agtcggacca ccaggagtag gtaaaacgac tacaatagca
    17581 aagttagccg caaaattagt ttttgaagaa aataaaaaag taggagtaat aactatagat
    17641 acatacagga ttggtgctgt agaacagttg aagatatata ctgatataat gaatattccc
    17701 tttaaagggg taataagtcc tgatgaaatg gaattggcac ttgacgagat gaaagattgt
    17761 gatgttgtat taatagatac aacaggtaga ggctacaaaa actctatgca aatactagaa
    17821 atcaaaaatc ttatagataa agctgaaact gataatatac atttagtttt gaattgtaca
    17881 acaagagaaa gtgatacaaa agcaattata gattcatata gaaatgtgaa ttttaaaagc
    17941 ttaataatta caaagctaga tgaaacaata acatatggct caatatttaa cataatgaat
    18001 tatgcgcaaa aaccaatatc atatattaca acaggacaaa atgttcctga tgatattata
    18061 aaacctaatg aagataagat tataaggtta ctattaggag ttgaaagtat atgatagacc
    18121 aagctcaaat tttgagaaaa atagcaatgg aaaaaagagg acttgatgag tttatagtag
    18181 agaatgagaa tgtacctaag ataattacaa tagcttctgg taaaggcggt gttggaaaga
    18241 gcaatctcgc tacaaatttg tctatatgct tgaccaaact taataaaaaa gttcttatat
    18301 tagatgcaga cataggaatg tctaatatag atataataat gggggtaaat gtaaaaggga
    18361 ctataattga tgtaattaat ggcgaaaaaa atatagaaga tataatttca cagactaagt
    18421 atggtgtaaa tataatatct ggaggttctg cccttaatca tatagaagac tttacagaag
    18481 ctcaaagaaa taagtttata catagcatag aacaaattca cgatgtggat tttataataa
    18541 ttgatacagg agctggtatg agcaaaagcc tattgtcatt tatatactgt agtactgaat
    18601 ttttcttaat aacaactcca gaaccgacat cattgactga cgcatatagt ttattaaaag
    18661 ctataagtaa ttttggcata aagagaagtg caaatataat aatcaatagg gtaattgatt
    18721 tagaagaagc aaaatctaca tacaaaagaa taaaaatggt tgtggacaaa tttctaaacc
    18781 ttaatctaga attgtatgga tatataatag atgacaaaaa agtaagttta tgtgtaaaaa
    18841 aacagatacc attcatttta gaatatccta caagtattgc atcaaaatgt attatagcca
    18901 tagcaaagag aatggtaagt cagtcaagag aagatattgt tgataataaa agttttctaa
    18961 gaaagatgtt tagtatattt aaaagctaag gaggcgtagt taatgaatag agaagaatta
    19021 ataaaagaga atatgcctct tgtaaagagt atagcaagaa aattttttat accagggaaa
    19081 ggttttgaat atcaagactt agtaaatagt ggagtcattg gattgattga tgcaataaat
    19141 aaatttgatg ctgaaaaagg ggcaaagttt tctagttact cttatataaa aataaaatct
    19201 gctatattag atgaaattag aaatcaaagt ccaatttcta aacataatct aactaaagta
    19261 aataagtata atagagtggt agaaaagttg caatctaaat tattaagaga gccaacttca
    19321 tatgaaattg caaaagaatt aaaggtttca gaaaaagaac ttcatgatat cgaaagtaat
    19381 atagatatgt taaatattgt atcattaaac tatgtaatct ttgaagatac aaatgaaact
    19441 gtacaagatg taataagtga tagagaagag gaagctccag aaaatataat agaagaagaa
    19501 gaaaagttag agattctatc caaagcaatt agtaatttaa atgaaagaga aaaattaata
    19561 ctttcactgt actattatga agatttaaat ctaaaggaga tagggaaagt gctaggtgtt
    19621 tcagaatcaa gagtttcaca actccacaga aaatcaataa gaaacttaag aaacaagata
    19681 aaagaactta aatattctat atagatggtg attgataaga tgatacaaat tatattaata
    19741 gtagtgtcta ttttaataat agtaactata gttataaatt tacataagga atcaaaaaat
    19801 aaagataaca gatactttga taaaatattt gagcaagaat atgaagcaaa tgatagatta
    19861 ataattgcag aaaaaagaag attcttttta gacaaagtac ttgcagaaaa aagaattaat
    19921 agtcatataa aagtttatga aaattataac ctaataaagg ctaatgaaga aagaaaggaa
    19981 aacatattta tagaatcaaa aaaggaagta agacaagaaa atgagttatc tcttaaagtg
    20041 aaacatctta taagttcagg ttatgatgat gtggaaatat gtaagatgct agatattgga
    20101 aagggggaac tgttattaat aaggagttgt tacaaaatat aaggatatta ttatgtgata
    20161 aaaaagtatt cttaggaata ggtattggta tattaatatc aactttatgt ataatgcttt
    20221 ttagtaataa tgatatgagt aaggcagaga tagaaaaaaa ggccagagcg ttaggcatga
    20281 aatatacaag tgagatgaaa gttttagaat agatgatatt gtttagacat ttacaaaaaa
    20341 ggaggtcata atgtgataag aggattatat acagcagttt ctgctatgat aactaatcaa
    20401 caaaagcaaa atgttgttgt caataaccta gctaatatgg atacaaatgg atataaaagt
    20461 aaacaacttt tgactaaaag ttttgatgaa ttaaagttgg agggttacaa taactacgca
    20521 aatggaacta aacaaaagca gattataggc gatataagtc caggtatttc tatggatgaa
    20581 actatcacaa attttaatca aggacctata aaaactactg ataataaaat ggatgtagct
    20641 attcaaggta agggattttt tctagttagt gatgcagctg gaaatcaatt ttacactaga
    20701 aatgggaact tcagaacaga caatcaaggt gaccttataa caagtgaagg gtattatgta
    20761 cttgggacga atactgctac tggtgctgta ggtcatataa atgtaggaaa tcaaaaattt
    20821 gaagtggcaa aagataatac aatatcacta gacggaaatg ctatgtataa atttaatatt
    20881 gtagatttta ataactacaa taacttaaaa aaagaaggag ataacttata ttctggagga
    20941 ggagctatga aagtaaataa tgcacttaca atacaaagtg cgatagagtc ctctaatgta
    21001 gatatggtat ctgaaattaa taatatgatg acaatatcaa gagaatatga ggcaaatcaa
    21061 aagataattc aggctatgga ttctaaatta gcaaaaatag caagtgagat tggttcagta
    21121 agataggtaa taggaggtga atttttatgt acacaatgtt ttatactagt aaaacagctt
    21181 tatcagcaaa tcaaagtaaa ctagatataa tatctaacaa tatagcaaat tctgattcaa
    21241 caggatataa aaaaataaat atggaatatt ctgatttagt ggatgaagta ctaaatagac
    21301 catcatatcc tacaaatggc aatgatatat cgactggaac aggagctaag gcaacagctc
    21361 ccatgagaaa ttatgaacaa ggtgcattgg ctcctacaga tagtaagagt aatttggcaa
    21421 tagatggaga aggattcttt agagtgataa gaaaagatgg tacatatgca tatacaagaa
    21481 atggtggatt taatgtagat gctttaggta agattgtaga tgataatgga aatatattag
    21541 atgtccaatt tgatgcagga tataactata ataatacaaa tatagatagt acaaacctga
    21601 cagtttcaag aaatggagag ctattcgtag ataataaaaa aataggaaaa atcaacttat
    21661 atcaacctat aggaactcaa aattttatat ctgaaggaga tagtttattc gtcgcaactg
    21721 atgcagcaca gattaaacaa gttgagaaag tcgatatagt tcaaggctat agagagaggt
    21781 caaatgtttc tttacaagaa gaatttgttg acttaatagc cactcaaaga gcatttcaaa
    21841 tgaactcaaa gggaataaag acagcagatg aaatgtggca aatagcgaat aatttaagag
    21901 ctaaataatt atgtaaggtg aggaatattc atgaatgata tgaaagagta cgattttggc
    21961 aaacctcaga gttattcaag agaaaattta acttgtttaa attttttatt agctgaattt
    22021 tgtaaaaaat ataaaaacta tataatttat gaacttaagt gtaattctag tatggttgtt
    22081 gatagtatag accaagtaaa ttatcagact tttttagata gagtaacttc ttcatgcgta
    22141 gtagtacaaa atgcaatgga acctgttatg aaaaactttt cttttagaat agatagaagt
    22201 gtagctgata tgtggattga tataacttct ggaggaactg gagttgtaaa ggataatgat
    22261 agagaactta cagaacttga taaaaaagtg cttttacacc ttatggatga tttagtaaga
    22321 aatatgcact tatttgaggg ttttgaaaat atacaagtat tggatacaca tacacatatt
    22381 aatttgccac aattatgttc tccaactgca ccagtatgcg ttgtagattt aaaagtgcta
    22441 aatgataatg agtatattgg aagtgtttct ctttgctttc catacaatgg tttagaggct
    22501 ctgttagaaa gtatatctgt tatagaattt tttgataatg atgtaggaga tgattcagag
    22561 gaatttaccc aaaaaatcta taatagtgta tctaatatag aattatcagt tattgcagag
    22621 ataggaaaag tatcaattaa tgttgaggat ttactcaaac tagaagttgg agatgtaata
    22681 gtaacaaata aaaaaataaa tgattatata gatatatttg tagaaaattc aaaatcatac
    22741 acagcaacac caggatttat atcaagtaaa aaaggtgtaa aaataaatga tgctgttgga
    22801 aaagaggtgt agttatggac aatagtaatc ttatttcaga tgaagaagca aagatgttat
    22861 taaatgataa agttgaaagt gaagttgatg gagatgatga gttagcctta gatgtctctg
    22921 aagttaatcg aaataatata caaaggatac ttgaccttaa gttagaatta tctgtagtta
    22981 taggaagaac taagatgact ctaagagata ttttaaattt acataaaggt tctcttattg
    23041 aactggatac tttggcagac caagatgtgg aaatactaat agataataag gttttggctt
    23101 atggaaaagt agtggtggta gatttaaatt ttggtgtaaa gataacaagt atagtaagtg
    23161 aagaagacat ggtaaaatct cttgtttaga gataacatca attaatactt aaggcatgag
    23221 gtggtactta ttttaaactt aaatgatata aaaaaagata tactaaaact caataaaaaa
    23281 gactcatgtt ctctatataa tgaatctatt aataatataa ataataattg tatatcaact
    23341 ggattaaaat ttttaaaaca agcaagagat ttaaacccag atgatgttga tatattaaat
    23401 ctcacaggtc ttgtaaactt actaaaatgt aattttgatg atgctgtaga aagtttctat
    23461 aagagttcac tttgtgaaaa aactgctctg tgtgaaaagt atataaacat gcttacatct
    23521 gaggagtttt taattttctt tgaaaagtac aatcaactta taaggttaat aaataaagga
    23581 atgtataaag aagctataca agggtttaaa cttataactg accaatactg tgatttaata
    23641 gaaccttatg agttgcttgc cttactgtat gacaaggaag aagaatatat aaaatttgat
    23701 gaatgtttag aagtattgaa gattattgat aaggaaaatt atttattaaa tcaagaaaat
    23761 aacacattat atatgtataa tagtgctaat atacaaacta aaagtaatat aaaagtagat
    23821 gattataaga atgagttctt acataaaagt agtagtaacc ataaaattaa tgattataat
    23881 gatgtaacta aagaaaagag ttcaagcaaa aaccagaaaa gttcagattc taataagaaa
    23941 aaaagtatta aaaaaaataa gtacctatat atagccataa tattaggaat attattagta
    24001 ggacaaagta tatatagttc ttataagatg aataatttaa ctgataaaat atctaaaaat
    24061 aatgaaacta aaaatgaaga gtcaatagat tctaacttga ctaagaaaga aaataatata
    24121 gaagaaaatg aagatatgag taaggcttct aatataaata agagtaataa aaacattaat
    24181 aaagaggatg atgtaaaatc taaaaaaaat actgtagaaa atgaagatga taaagattct
    24241 gacttattta cagaggatga attgatgtct aaggcgaata ctttaaaatc agaaaagaaa
    24301 actaaatctt ctatacaact atataagaaa gttgcagata taggtgaaaa taaagggaat
    24361 acatctgaag caacttatca agttgctatt ctcagtgaaa agttaaaaga ctataaaact
    24421 gctgaagaat attataaaat gtatgtagaa aattactcag aaaaagatgc ttattttgat
    24481 gaatcttatt ataatttagg tatgatgtac tataacaatg gtgatttaaa aaattctaaa
    24541 ttgacattaa agaagttggt taataaggta ccaaatagta tgtataataa ttcaaaagta
    24601 aaagaaattt taaaagaaga atagtta
//
LOCUS       DAMJWS010000064        24610 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMJWS010000064 DAMJWS010000000
VERSION     DAMJWS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.064"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(517..693)
                     /locus_tag="PC399_002584"
     CDS             complement(517..693)
                     /locus_tag="PC399_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593437.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
     gene            1268..2290
                     /locus_tag="PC399_002585"
     CDS             1268..2290
                     /locus_tag="PC399_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593438.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALN
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            2304..3191
                     /locus_tag="PC399_002586"
     CDS             2304..3191
                     /locus_tag="PC399_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593439.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRAGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            3197..3970
                     /locus_tag="PC399_002587"
     CDS             3197..3970
                     /locus_tag="PC399_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593440.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            4082..4807
                     /locus_tag="PC399_002588"
     CDS             4082..4807
                     /locus_tag="PC399_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593441.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLFAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            4811..6214
                     /locus_tag="PC399_002589"
     CDS             4811..6214
                     /locus_tag="PC399_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2593442.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            6225..7049
                     /locus_tag="PC399_002590"
     CDS             6225..7049
                     /locus_tag="PC399_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF2593443.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(7374..7994)
                     /locus_tag="PC399_002591"
     CDS             complement(7374..7994)
                     /locus_tag="PC399_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2593444.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNKLIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            8260..8592
                     /locus_tag="PC399_002592"
     CDS             8260..8592
                     /locus_tag="PC399_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2593445.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            complement(8712..9353)
                     /gene="pcp"
                     /locus_tag="PC399_002593"
     CDS             complement(8712..9353)
                     /gene="pcp"
                     /locus_tag="PC399_002593"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF2593446.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(9374..10306)
                     /locus_tag="PC399_002594"
     CDS             complement(9374..10306)
                     /locus_tag="PC399_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF2593447.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            complement(10328..10993)
                     /locus_tag="PC399_002595"
     CDS             complement(10328..10993)
                     /locus_tag="PC399_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF2593448.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            complement(11563..12036)
                     /locus_tag="PC399_002596"
     CDS             complement(11563..12036)
                     /locus_tag="PC399_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2593449.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(12039..12443)
                     /locus_tag="PC399_002597"
     CDS             complement(12039..12443)
                     /locus_tag="PC399_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2593450.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(12418..13047)
                     /locus_tag="PC399_002598"
     CDS             complement(12418..13047)
                     /locus_tag="PC399_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2593451.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMYSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      13412..13455
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            13556..14524
                     /locus_tag="PC399_002599"
     CDS             13556..14524
                     /locus_tag="PC399_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF2593452.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            14526..15089
                     /locus_tag="PC399_002600"
     CDS             14526..15089
                     /locus_tag="PC399_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2593453.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            15097..15786
                     /locus_tag="PC399_002601"
     CDS             15097..15786
                     /locus_tag="PC399_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2593454.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            15913..16641
                     /locus_tag="PC399_002602"
     CDS             15913..16641
                     /locus_tag="PC399_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2593455.1"
                     /translation="MMFDRVKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            16655..17329
                     /locus_tag="PC399_002603"
     CDS             16655..17329
                     /locus_tag="PC399_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2593456.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYIFKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            17355..18191
                     /locus_tag="PC399_002604"
     CDS             17355..18191
                     /locus_tag="PC399_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593457.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            18207..18878
                     /locus_tag="PC399_002605"
     CDS             18207..18878
                     /locus_tag="PC399_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593458.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            18868..20100
                     /locus_tag="PC399_002606"
     CDS             18868..20100
                     /locus_tag="PC399_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2593459.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            20250..20567
                     /gene="trxA"
                     /locus_tag="PC399_002607"
     CDS             20250..20567
                     /gene="trxA"
                     /locus_tag="PC399_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2593460.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            20584..21462
                     /locus_tag="PC399_002608"
     CDS             20584..21462
                     /locus_tag="PC399_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2593461.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            21732..22100
                     /locus_tag="PC399_002609"
     CDS             21732..22100
                     /locus_tag="PC399_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2593462.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            22103..22474
                     /locus_tag="PC399_002610"
     CDS             22103..22474
                     /locus_tag="PC399_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF2593463.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(22619..23074)
                     /locus_tag="PC399_002611"
     CDS             complement(22619..23074)
                     /locus_tag="PC399_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593464.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(23113..24378)
                     /locus_tag="PC399_002612"
     CDS             complement(23113..24378)
                     /locus_tag="PC399_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF2593465.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         9516 a         2721 c         3784 g         8589 t
ORIGIN      
        1 taaataaaat aaattaatca atatagtttt ttaatataaa caaaaaataa aaaacttata
       61 taaataaaat gcttgttttt cttgtattat ttatataaat attgaaattt gtctagttat
      121 acgtttttat tgaccagtta tgcagttgat attgaatatt ctaaatattg gtgttatttt
      181 ataggtggtt taaacatatt agggggattt tatatatgat ttgtatgatt tatagagaat
      241 gtataaaata gtaaaggttt agagccatgt ttagtggatt gcagaatatg catatagaat
      301 gattcttgta aattaagtta gacataggaa atatataatt tgtattggat gattaatttt
      361 atggtgtatg cttttaactt ctataatggg aaagtaaaga tgtactccca atacatcttt
      421 actttagcag aagttatagt tacttataaa gtacaaaaaa ttatatattt attagagtag
      481 ataaatctaa aaaggttttt ctagaaaaat ttagttttaa ttgtatctac ttactaataa
      541 taagacttga tgtaagcagg tttcaactac cgtttctcca tattctgata taaaatcatc
      601 taactgtgta tctaaaaaat tacaataatc atatgaacta ctgatgtaag cagttagtat
      661 atgtgtacat ttgtctattt ctccaataaa catattatcc atccccctaa aaaatttagt
      721 aaaaataaaa agtcgcattc tattagtcaa ttaacataat ttataaaatt ttttagtact
      781 agtttctata acttaactcc ttaaggttaa atattcccag ttttaccaat ttagttttat
      841 tctacaagta tataatacta gaagatgctt cattattcaa catgcttgtt tgagaataga
      901 aaatattcta aaattataat tgtttttaaa aataagattc aagataaata aagttatatg
      961 caactattta ataatctttc cataataagg acagtaggtt ctattcttaa aaaactccat
     1021 ctttaaattt aaagaataac tacaatttga acaacaataa aatttccaat caataaaaat
     1081 atcaaaaata ataaaaataa ttgaaaatgt ataatttccc agaaaaataa taaatgtaac
     1141 tccaataata aacacaccta taaattataa aataatttaa gctttattta aggttagtat
     1201 tatatttata taagatttaa gttttaatat atatgtgtaa ttaaaatgat ttaggaggag
     1261 aaatatattg gtaaattggg aaataaaaaa attggctaaa tctaagagta tgtttatatc
     1321 tctaggcata ttagtgttaa tactaatgat tagtatcttt attacgcctg ttttagaaac
     1381 agaaagttat tatatagata aagaaaaagg acgtatagaa gatacgagaa gtggtatcga
     1441 cataggtaat gagaaatttc aaaataagat taatgtttta aagcagtttt caaatcaaac
     1501 agatacaggt gaattttcta aaaaaattga attaatgtca aagaaaaaac ttgataatct
     1561 aagtgtgaat gaatataagg atattagttt ttggaaagta tttaactata gagttacaaa
     1621 ttcacttata aatgtaggaa tgttaatcat tatatctata attatatcta atatttacgt
     1681 agatgaagtc gttacttcgg taaaagatat catactatca tcaaaagaga agaaaaaagc
     1741 attaaattct aaaatatttg ttgctttatt agttccatta gtaatttatt ctgtttattt
     1801 aatgggaata tttattataa cttgtatgca acaaggagta cctgttaatg gagacttaga
     1861 atcttttaga atagtagaca atgtagttgc attgaaaact aatttaagta taacaaagta
     1921 tgtaatatta aatataggca tagagctttt gatgtttgaa ggatgggcca tgatagccat
     1981 atttgggtct tttataagta agtcttcaat atcttcaata agttttttta tatttattat
     2041 atctataact aagataatgt cggtaatcca tatattacct aaaaaattac tttcagttgt
     2101 atcaaatgta aattattatg atttgatatt tggttttaat aaaataatag gaaattatct
     2161 aggtgatatt atgatattta aagtgaatat agacattgtc aatatagcaa taggactttt
     2221 gatggcaata ttgataggtg ctatgtcatt atgtttttta gtaataaaat cagattatat
     2281 aagtagataa aaggggagtt aagatgaata aattagaaat agaaaatctt tcaaagatat
     2341 atggtaaaaa agtagcaaac gacaagatta cagtaactct agaaaatgga gtttatggtc
     2401 ttctaggacc aaatggagca ggaaaatcta cattaatgaa acaaataaca acgttgataa
     2461 aacctgatga aggtcagata ctttataatg gtgaggatat atttaaaatg gatgctgatt
     2521 atagaaatat tttaggatat ttgccacaag aatttggagt ttataaaaat tttacagcaa
     2581 aagaattttt acagtatatt ggagctttaa aaggtttaag gggaaaatat ttaaatagta
     2641 agataggaga attattagat ttagtaggtc tttatgatgt gagaaataag tctataggaa
     2701 aattttcagg aggtatgaag aggagagctg ggatagcaca agttttacta aacgacccta
     2761 agataatagt ccttgatgag ccaacagcag gtcttgaccc acaagaaaga gctagattta
     2821 ggaatttaat agcaaaaatg tctagagata agataataat actttctact catataattt
     2881 ccgatataga atcagttgca aaggaaacaa taatgattaa agatggcaaa ctcctaatga
     2941 aaggaacaca caaagacata ttaagtcaca tggaaaataa agtatataat gttcaagtta
     3001 aaaatgaatg tgaagttgat gatatacaat ctaaatataa ggttgtaagt attcaaagtg
     3061 atattaattc aatagtactt agaattgttt cagaggatat tccaagcgag gataatatac
     3121 aaccagttca agcaaaattc gaagatgtat acatgtttta ttttgattta gaagatgcaa
     3181 gggaggtgta gtagaaatga tgtatttagt taaagctgaa atgataaaaa tattaaaatc
     3241 aaagagtgtt tggataattt ggctgttttt aatatttttt ggattttttc tgattagaaa
     3301 gtttgatgtt ttagatactt atgcagatat attttacaag atagaaggct caatacctct
     3361 aataggtttg ataatgttta taataacttc tggaaattac attaaagaat atgattctaa
     3421 tatgacaggt ttaataaata caactaaaaa gggaaaaaaa tcagtagtat tatcaaaact
     3481 agtagctaat ggattaattt tatctattat taatatttct tttatattgt tagtaggaat
     3541 tagaggtttt agtttttttg attttaaaaa tttaaatgaa tcgattcata acttatggta
     3601 ctttggaaat agtaatttaa atcttacagt aatacaaatg tatataattg ttatattgac
     3661 tactattttt gcttctttta tatttgcaca aataggctta acactttcat ctatatttaa
     3721 atcggctgta attccattta tactaggtgg attgataatg gcaattccat actttagtgt
     3781 aggttttata ccagataaag ctataaaatt tatgtcagtg actccaaatt ggattatgat
     3841 gagccaacaa atggttaagt ataatgtacc aagtattttg atagtttttt ctatagtaat
     3901 ctcaattata ttaatgatag tgttaactaa aataacatat gaaaatttta catcaagcaa
     3961 aaggttttaa ctaaaaataa tagtaatttt atttatattt tagtttaatt aaaaataaga
     4021 taaatgaatt ttagataaaa tatatgctaa tatatatgta aattattaca ggaggttgtt
     4081 tatggaaaat cgtgttttaa taatagatga tgaagtagaa atattgaagc ttttagaaac
     4141 tgtcctaaaa aaagaaggcc ttaataatat atatactgca aaaactaaaa aagaaggatt
     4201 agaattattt aaaagtatta atcctgattt gattgtattg gatattatgt taccagatgg
     4261 cgaaggttat gatatttgta aagaaataag aaaaacctcc aattctccaa taatattttt
     4321 atcagctaaa accgaagaat tagataagtt acttggacta gcgattggag gagatgatta
     4381 tgttacaaaa ccttttagcc ctaaggaagt ggcttttaga gttaaagcgc atttgagaag
     4441 attaagttat tttagtgatg ctcaaaacga aagtaaaaat ctaaataata atgaagaaaa
     4501 aattatatca tttggcccat atatattaaa tgaaagtagg gcagaactta taaaagatgg
     4561 aaaatctata ggactgtttg caaaggaact aaaaatccta tctttatttg cacataatca
     4621 aaatcaaatt ataagtaaag aaaaactatg ggataaagtg tggggagaag attatgttgg
     4681 atttgacaat actataatgg ttcatataag aaaaattagg gaaaaattag aggataatcc
     4741 ttcaaaacct gaatatattc tgacaataaa aggtcttgga tataaattag cagtaaagga
     4801 agattagaat atgaagtgga agattactag aaattttata tttacaattg tgtttgtagc
     4861 tatatcagta gtaattataa atatcatatc tatattgtat gtgatttcaa ctaatagctt
     4921 ttttaaagtt gtagattctg gaaataatcc tgaagaattt gcacgttctt ttgaaaaaga
     4981 tttatatgaa aaagatggag aatttaaact atctaagatt ggagctgaaa aattagaaaa
     5041 atcaaattcc tggatacagg ttttaaatga tttaggagag gaagtttatg gagtaaatgt
     5101 gccaaaggat actccaaaaa aatatacacc ctttcaaatg gtgaataatt acaagtatat
     5161 agaaacaaag tatgttaatt ttgtactaga aaaatattta aacaaaaaac atttaaacat
     5221 aatagtaggt atacctagta gagatatatc aagaataatt ttaacctatt ctcaaaataa
     5281 cattaaaaaa actttaaata aagttatcat tataacacta gtaattgata gcgtggtagc
     5341 tttaggtgta ggatatttat ttagtagaaa acttacaaaa ccaatttcaa gcgtactatg
     5401 gagtattgag actatggcta atggaaatta tagtttatat cttaaggaca gaggtatata
     5461 tgaagaagtt tttaaaaata taaatatgtt agctgatact ttaagagtaa atgaagttga
     5521 gagaaaagaa aatgaagagt tgagagagga atggctggca aatataacac atgatataaa
     5581 gacacctcta gcatctatac aagggtatgc agagataatt aatgacaaag attatgagtt
     5641 tgaagaagat gaaatacaag agtatacaga aattatatat aataagtcta agtatataaa
     5701 agatttagtt gatgatttaa atttatctac tcgattaaaa aataatacta tagttctgga
     5761 caagaaaaaa ataaatttag tcagtttagt cagagatata ataattgata ttttaaatga
     5821 taatagatat aaaaatagga atattgaatt tgaaagtaat gaagatttaa ttgaagtata
     5881 tgtagattca atcttattca gaagggctat aaccaatctt atatttaatt caatagttca
     5941 taatagcgaa ggaactttga taagtgtaga aattgtaaaa aaagataata tagaaattat
     6001 aataaaagat aatggaattg gaataagcaa aagtgatttg aaacatattt ttaaaaaata
     6061 ttatagaggt actaatacag gtgagatgca taaaggttca ggtcttggta tggctatttc
     6121 taaagaaata atagagattc ataaaggtaa aatttatgta agtagtgaaa ttggaatagg
     6181 aacaaaaatt attatagaga taaaacaaaa ttaggagata tgatatggat ttttttaaaa
     6241 aaggtataag tgggtttact attaaaattt tagcattaat atttatgaca tttgaccata
     6301 tagcagcatt tatgccacaa acaatgcaaa taccaatttg gtttcattgg gtaggcagaa
     6361 tatcagcacc actgtttata ttcatggctg ttgaaggatt ttatcacact agtaacagaa
     6421 aaaaatatat aagtagattg tatatttggt ctgttattat ggctatagga aatcaaataa
     6481 ttaataatgt attttcacat ccagaaggag ctataattat aaataacatt ttttcaacat
     6541 tatttttaat agcgatttat ttacaagcaa tagagttcat aaagaaattt agaaaagaga
     6601 aagaaattaa atattttatt ataggattat tgatgataat cattcctata attttaggaa
     6661 tatttacagt agctttactt tttaaagtca ctaacagagt tattgcttta tttatgattt
     6721 tggtaccagt accattttta gtagaaggtg gaccaatttg gattatactt gggattattt
     6781 tttatttgtg tagaggtaaa aagttcagtt tgagtatttg ttatgtttta atgtgtatat
     6841 ttatatttac aacaatgtca aatggagatt atagtttaaa aaattctatt ttacaaaatt
     6901 accaatggat gatgattgct tctctacctt taatgttact ttacaatgga gaaaaaggga
     6961 agagtatgaa gtatttattt tatttatact atccaatcca tgtatatata ttgtatatct
     7021 taggaatata cttaataaat ggtttttgat tgtaagtgat atttttaata caaaatgaac
     7081 agtatgagga ttgttttagg tctattaaaa ttgttattta gatgagattt gtagaatatt
     7141 taaatttatg agcagaaatt cagttattgt aatttcaata acattagaat ataatattat
     7201 aatttattta gaaatatatg atgttataca tggtttggtt attaatgtaa taataaaaca
     7261 gaggtttgat ataaaattca atacctctgt tttagtgctt tttatcctac atttatgctt
     7321 attttattta taaattagtg ttttgtttta attaatatta gtacattttt tatttatttt
     7381 ataaaattag tcataattgt ttcttcatat tgtggttctt taattccagc ttttttacct
     7441 gctgatatac acttaagaag ccatgccatg ttttctccta gagttcgcat agtctgtaat
     7501 ccttcttcat ccttcataac atcctctgga gtaaagccat gtacctggtt ccaatactgt
     7561 gatgatacaa taggcatatt gctgattgaa aaatatttat ttagttggtc aaatgttgca
     7621 cttgcaccac cacgtctaca tgacacaact gaagcaccta attttgtagc catgcgactt
     7681 ccagctatga aaaatagacg gtcaagaaaa gaagttaatt gaccagttgc actagaaaag
     7741 taaacaggag aaccaataat aataccatca atattatcca gatcttcaat caatttatta
     7801 actttatcat ttcgaataca ttttccagtt tcaaaacagg atgcgcaact aatacatcct
     7861 gggattgttt ctttacctaa atacagtatt tctgttttaa tatcgtgttt actcaatact
     7921 ttagtaatct catttagtgc tgtataagta catccatatt ggtttgggct accgtttatc
     7981 aataaaactt tcattataaa tttcctccaa tgaattttat ttaatagatt ttctaagaga
     8041 tattattttt tataaaatag gattttcatg aatattatca atttatatag aaatgtctaa
     8101 tcttagaatc ctatttaaaa taactttttt tttagtaaat acacctccta ttataaaatt
     8161 gactttcaat tattcttaca ctataatttt attataaatt attctaaaaa caagtacgca
     8221 catttttgtt caatactaac caaaaggaga gtgtaatata tgagtataga ctgtatatca
     8281 aatacagatt taagtgaaac aggatttagt tatacacttt cactgattag tggtaaatat
     8341 aagatgataa ttctttactg tcttgtagaa tttgaagttg taagatataa tgcattaaaa
     8401 cgttatataa atactatctc ctataaaact ttgagtttat cattaaaaga attagaagca
     8461 gataacttga ttattcgtac tgaatatcct caaatacctc ctaaggtaga atatagtcta
     8521 tcagaaaggg gaaaatctct gattccaatt ttagatgcaa tgtgtgagtg gggagagagg
     8581 aatagacctt aattttgtat ctgtagatac cataaaaaga ctgtgagtaa aattgtttga
     8641 ataatctact cacagtctat atttaaatta atagttagta tttttaattt ataaaaatac
     8701 taaaaaattt attatgaaat ttcaccacca atttctttta catcttcttt atatataata
     8761 caagcttcta tagctaattc taagcctttt actatttctt ctaaagacat gaatggtgta
     8821 ttttttttat ctattacttg tgaagttgta tatgggatgt gtataaatcc accccttata
     8881 ttaggatatt tcttgtctat catataaaga attccataca tcacatgatt gcaaacaaat
     8941 gttcctgcag tgtttgaaac actagctgga attttatttt cattcatatg ttttaccata
     9001 gcttttacag gcaattttga aaaatatgca ggttcaccat cttcttttat aatagtatct
     9061 ataacctggt taccctcatt atcttttatt ctaaaatcat ctatatttat agcaactctc
     9121 tcaggtgtaa tgtcaaatct acctccagct tgtccaactg atattactat atctggattg
     9181 tgctcttgaa tagctttatc tatagcttca acagattttg tcataactgt tggtatagtt
     9241 atctttatga tttctgcacc gtttatatta ttattgaccc tttcaactgc ttcttgagct
     9301 gggtttattg gttctccacc aaatgggtca aatcctgtta acaaaatttt cattatttta
     9361 cctcctaatt atattaaaat cctaaaaagt acattactaa tatgtgggct ataattaata
     9421 ccaaagcaac tggagcttga tattttataa caatataatc atttttacat tctagcaagg
     9481 ctgctggaac tatattaaaa ttagcagcca ttggagtaag tagagttcca caatatcctg
     9541 ctgttaatgc taatgctcca attatagcag gatttccacc ttgagacaat acaaaaggaa
     9601 taccaattcc tgccgttatg actgaaaatg cggcaaatgc atttcccatt atcatggtaa
     9661 atataaccat acctaataca taagctataa caccaaataa tctgctatca gcagggacta
     9721 caccagatat catggttgaa ataacttcac cgactccggc ttgagtaaat aaagctccca
     9781 atgcaactaa aagttgtggt aacatactag caggtcccat ttgttgaagt aatcttgagc
     9841 catcttgaac tgatgtagca ggttttgcct tagttattat aagggctgat attaatgcaa
     9901 ttattgaacc cacacctatt gcaaattgag aagaattatc aaaattagaa aggctcatag
     9961 ctactactac tgcagcaaat gctaatatta atgatggtat aaatatttta tttttaagtt
    10021 tgtctgcttg ttcttttcca aaattttcat ctggttcttc atactttgca aatacaactt
    10081 gtttagaaag tgttaataaa ctagataata ttaatattat tcctattata ttggctggaa
    10141 tcaatctacc aaatatgaat ggcagtgata taagtatcca aaataatgat gtaccatatt
    10201 tcttattgtt atttatggta gtaaaagact tatatgctat aacaatcatt aaaaatccca
    10261 ttagtgcgta aaagatttct aatgatatgt ttataaaatt ttgcattttt attcctccta
    10321 aataaaacta ttttttatga cttttgttta attttttatc atataaggta aactggattg
    10381 tagccactac aagtgctatt actgccataa tagtactagc ctttacgata ttaagtccag
    10441 ttacattgta tccaaattga gtcagagtag atgctataag taatactcca ctactacctg
    10501 caaataagtt ttgaccataa aaatttccat agttttctga tgcagcagat aatgccttta
    10561 tggcttcttc atccgattct tttagttcat cactattact taaaccagca gcttgtgcca
    10621 taggattaac aagtggtctt acaaattgag gatgtccact cattcttatt gatagagcac
    10681 cagctatttg tctagctatc atgtatatag taagtacttt tcctgttgta agtcttttta
    10741 atttttctat taaagaaaca gctctttgtt ttaaaccata tctttctaat acaccgatta
    10801 ctggcaatgt taatatgaat aatgatacac ctctgtttga gacaaaagaa tctcctaaaa
    10861 ttgtaaaaat ttgattaaaa tctaatcctg caacaattcc tgttgccact cctgctaata
    10921 atacagtaaa taatgtgtca attttaagta taaatccaat tacaactatc aatataccga
    10981 ttagttttat cataaattgt tctccttcca tttctatatg ttttaattta aatttattta
    11041 atggataaag ttaaactctt tttatagtat ttgcgttaat aggactatta ttataaaatt
    11101 taataaaaaa tagggaagat gttttaaagt atcaaagtaa ataatggttg tgtataggaa
    11161 tttgaataac aaagattata ttttaagcta atatgaattg attttgtaag tctatagttt
    11221 aagtatatgt caatatttga aataggtttt aaattagata ttatattata aaaagagaat
    11281 gtgccaaaca tagagacaag tgtatttttc acttttgaaa tgataaaaat atatttgatt
    11341 tcatacaaaa agagagtgtc taatgaattt tttagttcat tagacactct ctttttgtta
    11401 tgattaataa aacatatttg ttatgattaa taaagcatat attaagtgct agtttcatta
    11461 gatttcacta agaaaaataa aattttgata aagtttaaag actattttga gcaatattac
    11521 ttataaataa aagaatttat catagaaata attagtttta ttttagctat taagatttat
    11581 taaaaaatta tctatattat cctgtgatat tctaaaactg tcataattac cttttgaaac
    11641 agccttcata ataagtttat ctaaaaattt cattttttct aatcgagctt cactaccaaa
    11701 aattccaata gcatttgcac tttctaataa ggatgatgga aaatttgatt ttaaatattt
    11761 atctgcttgt tcagaaaagc cacagcaaag gaaaatgcca acttgttttt tgtttaataa
    11821 ctctacatta tcattacata gcacttgtat tttttttgaa actgaaccaa catatataga
    11881 acttccaata ataattttat caaattttga taactcaatt ttattattat tattaatatc
    11941 aacaatagtg acattatctg atagtttatt ttttagtata tttgcacagt cttttgtaca
    12001 accatattta cttgaatata taataatagt attcataatt attctccctt tcttaaagca
    12061 ttttctacag cttttcgtat agtattactg ctataagttg cagaagttat agcatccaca
    12121 tctaaagact gttgtttaat aatacttgtt gtaatttttt cagcaggttt acctaaaaga
    12181 tggtcgtgtt ttaagatatt aatattttgg attttaccat tattaacttc aacagacaca
    12241 gtagctttta ccaagttatt atcagcactt ccagtataaa tgccattttt aacattatta
    12301 atatctatat cttttactac caagttttct ggctttaagt tgcttacaat tacaataata
    12361 atgcatataa atacaaagaa tagagcaata gatattttta catatttttt ctttttatta
    12421 gtagccttgt tgcgaatttt cattgtaaag tcctcccact aaaatatcta agaaattttc
    12481 tataagttga gaaaaattat gtaaagaata tagtttattt tcaacaacac ataaaacgaa
    12541 tcctaacaat gcacttataa gcatccatga gcttttatct atattttttt tgaatattcc
    12601 ttgttcaatt ccaatggaaa ggaatttatc aagcattttt ataccattat tgctaggcgt
    12661 attgtttgta ttttcagatg caaatataat attcattcta ctatacatga tagattttgc
    12721 catctctggt tcactagaaa gagtttttat aaagctagtc ataatttcta tgatttgttt
    12781 atctattggt agtgatgagc aattattcat aatgtcaatg gcattacttt ctactgtgtt
    12841 ataaagttta ttggtcatat cgctgataat ctctgctttg tctttgtagt aaagataaat
    12901 tgtagttgga gaatattcta tttttgtagc aatttttctt attgataaat tttcatatcc
    12961 atgttggttt ataatttcga tgctagcatc cataatcttt ttcttcattt cttctttttc
    13021 atttttacgt ctttctttga tactcatata atcacctctt tgcttaacgg tgttaagtta
    13081 atttacactg ttattgtata atttaatttt aatactgtca ataaattatt aaataatata
    13141 agttaatata tttttatgga taataaatta atgttattaa ttgtaatatt taagatatta
    13201 ttgtataata ataatcggtt taaatgcgcc acatcttatt aaagttggtt cataattcta
    13261 aataaacttt aataagatac attaaaatat aataaattct tttatgctat aaaatttatt
    13321 ttatagctat taaatcagat gtttaaaaat tttttaacag tctatagttt ttaagagtct
    13381 atagtaagta gcctaaaaat atgattttac atctgtactt gttcgagaac tccaagtata
    13441 aaaaactaag gaattatata gtggcagtat ataattaata cccttagaca cacctttttg
    13501 aaagtggaaa aaagatgtgt agttctcctc gaccacaaat caaggaggat aaaaaatgga
    13561 aaagagaaaa gtaataattg attgtgaccc aggaattgat gattctttgg caattcttct
    13621 ggctttaaac tcaccagagc tagaagtaat tggaattacc acatgttgtg gaaatgttcc
    13681 agcaaatata ggtgcagaaa atgcactaaa aacacttcaa atgtgttctt cactaaatat
    13741 tccagtatat ataggagaag aagcaccact aaaaagaaaa cttgtaacag ctcaagatac
    13801 acatggagaa gatggtattg gagaaaactt ttatcaaaag gttgtaggag ctaaagcaaa
    13861 aaatggagca gtggatttta taataaatac tttatataat catgaaaaag tatcaataat
    13921 agcacttgca ccacttacaa atatagctaa agcacttatt aaagataaga aagcatttga
    13981 aaatctcgat gagtttgtat ctatgggagg agcatttagg attcatggaa attgctctcc
    14041 agtagcagag tttaattatt gggtagaccc acatggagca gattatgttt acaagaattt
    14101 atctaaaaaa atccacatgg taggtttaga tgtaactaga aaaattgtac ttactcctaa
    14161 tattattgag tttataaata gacttgataa gaagatggca aagtatataa ctgaaataac
    14221 tagattttat attgatttcc attgggaaca ggagggaata attggctgtg tgataaatga
    14281 ccctctagca gtagcgtact ttatagatag aagtatatgt aaaggatttg aatcatatgt
    14341 agaagttgta gaagatggaa tagctatggg tcagtctata gtggattctt tcaatttcta
    14401 taaaaaaaat cctaatgcaa ttgttctaaa tgaagttgat gagaagaaat ttatgtacat
    14461 gtttttaaag aggcttttta aaggttatga agacattata gactctgtgg aaggagtgat
    14521 atagtgtgaa aagaaaaata tgtgtaaaaa ctattacact tatagctttt ggtatttcta
    14581 taaatataat aggcgctttt atagctatgg gattaagatt acctgtatac ttagattcaa
    14641 taggaactat aatgatagct tcacttttag gacctaaata cgctgttgtt acaggtgtgt
    14701 ttggaagttt aataagtgga attacgtttg atgtgtattc tctctatttt gcaccagttc
    14761 aaatatcaac cggattactt gctggaatag catttaaaaa gaatttttta aggggtttaa
    14821 aaactccact aggagtactt ttatttgcca ttccaacatc tataataagt tcaataattt
    14881 cagcattttt atttggaggt atgacctctt caggttcatc atatatagtg caaatcttga
    14941 aagtgttagg actgggggat gtttttagtg tgtttgtaac ccaagtattt actgattatg
    15001 gagataagct gttagcagtt gttttagtga atttaggatt aaatgcagtt ccaaaaactt
    15061 taaaggtatc attgactgga ggaaaataga tagaaaatgg atagatatag tgagataaca
    15121 aataaaaatc aaagagaaat tgttctgctt aaaggatttc cttgtatatg gggaaagtgt
    15181 tctttttgtg attatataga tgataattct aatttagaag aagagatgaa taaattaaat
    15241 ctaaaggttt taaaaaacgt aactgggaaa tatggagttc ttgaagtcat aaattctgga
    15301 agctgttttg aattacctaa ggatacttta gaaaaaataa agtgtattat aaaagagaaa
    15361 aatataaaaa aattattttt agaaagtcat tggtcttaca aaaataggct taaggaaatg
    15421 agagagtatt ttgaaattcc tgtggtattt aaaattggag ttgaaacctt tgacaatgat
    15481 tttagaaata atattttaaa taaaaatgca aattttaaga ctccacaaga tgtaaaagaa
    15541 tattttgatt caccttgtat tatggttgga ataaaaggac aaacaaaaga gatgatagat
    15601 aaagatatag agattatcct aaatactttt gaaaaagcga cagtgaatgt ttttataaat
    15661 aattcttcta gtataaaaag agatgaagag ttagtgaaat ggtttgttag taagtataaa
    15721 ttcttggatg aaaatccaaa tatagaagtt ttatacaata atacagattt tggtgtagga
    15781 gattagcatt aatttagttt aaatttaaaa ttgaaaagtt tcttatgttt ttgaaaaagc
    15841 ttctaatagt tagaagcttt ttttgtagag ttttatgtta caataagaaa aaaggaatta
    15901 aggagcaatt aaatgatgtt tgatagggta aaatggaata aattggatta taatgaactt
    15961 ttagaatata tgatgtctat acaagatata aaatatagag attttaataa aaagcttata
    16021 cctggaacag aaaatataat aggtattaga gttcctaatt taagaaagtt atcaaaagaa
    16081 ataagccaag ggaattggaa ggaattttta gaagtagcag aggatacata ctatgaagaa
    16141 gttaggctac aaggtatggt aattggcaat ataaatagta attttgaaga aactttatac
    16201 tatgtaaaaa gatttatacc taaaatcgat aattggtcag tttgcgatgg attttgttct
    16261 gatttaaagt ctatgaaaaa atataaagaa aatatgtatg atatacttaa aaaatatgtt
    16321 tattcaaaga atccatggga aattagattt gcattagtta tgtttttaat atattatgtt
    16381 gatgataaac atattaaaga aatatttgaa tactgtaata atatacaaag tgaagaatat
    16441 tatgttaaaa tgggtatggc atggttgcta tcaatttgtt ttattaaatg tgaagaggaa
    16501 acttttctct atataaaaaa taataattta gatgatttca cttataataa aatgctacaa
    16561 aaaataattg aatcaaatag agtagattta gaaaagaaga atataataag aagtatgaaa
    16621 agaaagacaa gaagatgttg acggaggaat attaatgaat aacaatgaag acagaattga
    16681 caaattttta gaacttcaaa gcaaaggatt taaatttgca gagatagctc aaaatctaaa
    16741 gattagtcaa agtactttaa gaactttttt aaataaaaaa ggatataagt cagaaaaagg
    16801 taaatacata tttaaagacg aattaaatta taataaaagt ttggataata gaaacttgga
    16861 agaagagaaa gctaaggagt atgaaaaaag taaaaagaat actataagta atagaaaaaa
    16921 agtaagcacc aaaaaaaaga tttctaatga aaaggcttta agtaaaagtg ttgaacaaat
    16981 tgagtttaat gatacaggtt taaaaaatgc aaagccaaaa caaaatatta agcctaagaa
    17041 agatagaaaa ataaatataa ctcaagaaga tttagataag ctttgtgaag tatatgattg
    17101 gtatttagaa gtaaaagatt acaatataaa gcctaaaaaa gtatctaata aaaaagatgt
    17161 acatatagat gatgaacaca tagatgaatt aaaaacaaca agaatacgta tagataaaga
    17221 aatatggaat gattttgaaa gattatgtag taattcgaat tttgaaaaac atcaaatact
    17281 tacacaggca ttgaaagaat ttatgaaaaa gtataaaaat ctactataaa atactactat
    17341 atgaggtgct taatatgaat aaaatagtaa aaaaatcaat ttctctaatg attatattaa
    17401 caatatttat ttttatgttg acagcatgtg aaaaagatga acagcctgat tcagttgata
    17461 ttcaaacaga agataaaaat gagataaaaa tagatgaggg aaatgcaaaa gtattgaata
    17521 taggaaaatc agaaattata aatatagatg aaggtgataa agtagataca tcaacaaagg
    17581 tagaaaataa ttcaacattt aatatatcca atgttgaatt gatatataat gaatatgatg
    17641 agaataaaaa aattatatct tcggatagta aggctttgtt ggacatgact ttaatgcctg
    17701 ggaaagttgc ttacatagaa tgtgggcata aaacttttgt aaagggtgta gaagtttatg
    17761 catatgaata tgaggcagag ggtaagattg tgtatgttaa tttaaaagaa aatactatta
    17821 atataagaaa taataatatt aagctagaaa atagtagtca gtatgaggtg ctatctacat
    17881 cagaacttaa aaaagtaaat gaatcaaatg agggaattac gtatcaagta aaagtaaaaa
    17941 attcatcatc aaaagattta ggaaatatta ttcttaagac agcagaagtt aatgaaaatg
    18001 gagaatattt aacagtaaat cgtgtgccat catataagat tttaaagcct tcagaagaga
    18061 cagatataga tatattgtgt tcaacaaagg caaaaagtgt tgaaatagtg ggatatacgt
    18121 atgatgatat aaaagaaaaa gccaatgtgg atattgactt aaaatctcat aaagttaaga
    18181 tagacaaata aaggtagggg atacttatga aggttttaat agttgaagat aataagatac
    18241 ttttagaaag tgtagtagaa gaacttagca agcattttga aactgaaaag tgtgaagatg
    18301 gagaagaagc tttatactta gtaaatcaaa atatatatga tttagtcatt ttagacttaa
    18361 tgcttcctaa tataaatgga tttgatatcc taaagaaaat gagagttaat aatatagata
    18421 cacctgtact tatattgaca gcaaaagaaa cattagatga caaagtagaa gcatttacta
    18481 taggagctaa tgattatttg acaaaacctt tttatatgga agagttagta gctagggtat
    18541 acgctatatt aaggactaat ggaaagataa aagaaagaaa cggattagaa tttaaatctt
    18601 tatatttaga tacactagaa aaaagagtgt atattgaaaa agaagaaata aaacttcaaa
    18661 acaaacagtt taacttgctt gagtattttg tattaaataa aggttcaatt ttattaaaag
    18721 agcagattta tgatagaata tggggaattg attcagatgc gactattgaa atagtggaag
    18781 tatatgtaag taatctaaga aaaaaactaa gtaaatatgg atatgataaa tatattaaaa
    18841 ctaaacgtaa ggtaggatat atattcgatg ataaataaga atgtatttac cagtactaaa
    18901 aatcatttaa taaaaatgta tattattgta gtaggtagtt ttttaataat cttttctata
    18961 tttatatact catattttag gggacttaca tatagtggaa ttgatagtga gataaatgat
    19021 gaacttgaat atatagtctc tcaatttaaa agaacttcat ttttgaaccc aataagactg
    19081 aaagatccta aagatatggt gtatgtgtat gaagatggaa ggataagtta ctatactcaa
    19141 aatgaatatt ttgatgagtt acttccagat agaagactag ataaaaaaaa ttcgtttttt
    19201 aaatacacag aaaatggata tacttttaga gaattaaatg tagatgtagg tagataccaa
    19261 atacagataa ttagaaatat agattctgag atgaattctt taagacagct tacatctgtt
    19321 cttataattg gaatattaat ttctgtaata attacttact ttgtggctgt atatctcact
    19381 agaaaagctt taattccaat agaaacagca tggaaaaatc aagctaagtt tatacaagat
    19441 gcttctcatg aattaagaac accaataaca atagtttcat caaaattaga aagtatgtta
    19501 aaatccccag aaagtacagt aaatgatgaa gttgaaacaa tagctactgc aatgaaggaa
    19561 actagaagac ttaaaaaaat gataactgat ttattgtctc ttactaaaga ggactctatt
    19621 gttaaagtta atttagaaga aatagattta gaaaagttat tggaagaaat atcagaagac
    19681 tatattgaca ttgctgagtt tcaggaaaaa agattcgtgt ttaattctaa acttaagaat
    19741 aaagttataa taacagataa aaataaatta agacagctta tattaatttt cattgataat
    19801 gcctttaagt atacaaaatt gggtgatgag atagcattag aattgaaaga agatatagag
    19861 gatgaggtaa ctttacttat aagtgacact ggtataggca taaaaaaaga agagatacct
    19921 cttatatttg atagattttt tagaagtgaa aatgtaagaa ataaagattt agaaggttct
    19981 ggaataggac tttcaatagc tcgcatgatt agtttaaact tatctattga tataaatgta
    20041 acttctgatg tagatattgg aactacattt gaattgtcaa tacccaaaaa gttaaaataa
    20101 taaaaatata aaaaattgtt tttagaaatg aagaatgggt atactaatta gtataaaata
    20161 taaaaagacc ttataaaagt aaatttatat ttataaattt catagtataa ttatataggg
    20221 ataataaagt ttaataggag gatttaaaaa tggcaaaagt tataaataca tctgaattta
    20281 gaagtagtgt tgaaggtagt aagggagtag ttgtagttga tttctttgca acttggtgtg
    20341 gaccttgtaa catgttggga cctgtatttg cagaattagg tgaagagatg aaggataaag
    20401 ctagatttgt aaaagttgat atagatgaga gcttagaaat agctcaacaa ttcaatgtat
    20461 ctacagtacc aactatgatt atatttaaag atggaaaacc agtagaaact ttaattggat
    20521 tcatgccaaa gaacaaaatt gaaatgcaag taaaatcata tttataaaaa atgggtgagt
    20581 attatgagat atgatatagc tataatcggc agtggtccag ctggattgtc tgctgctata
    20641 aatgcaaaaa taagaaataa aacaattatc atgtttggta atgataattt aagtaataag
    20701 cttgttaaag ctccttcaat agataattat ttaggttttt atgatataag tggtgatgaa
    20761 ttaaaagaca agtttaaatc acatatagat agtatggata tttcaattga aaataaaaga
    20821 ataaataata tttatgctat gggagaatac tttacaataa tgtctggaaa tgatatgtat
    20881 gaagctacta ctgtaatcct tgcaactggg gtagagtata ctagaccaat caagggtgaa
    20941 gaagaatttc taggaagagg tgtgggctac tgtgctacat gtgatgcacc actttataga
    21001 aataaaaagg tagcagttat aggatataat gaagaatcta aagaagaagc aaatttttta
    21061 agtgaactca cttcaaagac ttattttata cctatgtaca aaaaagataa tcttatgaga
    21121 agttcagata acctagatga ttctattgaa gttatacatg acagaccagt acagatagat
    21181 ggagataaac ttgtaaacaa agtatctttt aaagaaaacc acattgaagt tgatggagta
    21241 ttcgttataa aagatagtac agctccaagt gcattggttc ctggaataga gattgatggc
    21301 attcatatta aagttgacaa taatatgaag actagtatag atggatgctt tgctgctgga
    21361 gattgtgttg gaaaaccata ttcttatata aaagcagctg gtcaagggca aatagcagct
    21421 ttaaatgctg tttactattt agataaatta aagagggctt aatatttata ttgattaact
    21481 ttttaaagat atttataaaa aactgtcttg aaataatttt attatttcaa gacagttttt
    21541 tatttttaag ttattaataa aagtatttta aaatctatat gtaattactt ttattaataa
    21601 aactcaaagt tatattagta aaaattggaa ttatttataa tatactccaa aatttaaaaa
    21661 taatggttga aaaatctaat taagagtact ataatttaat taagtaatta cttaatcaaa
    21721 acgaggtact tatggaaaac ttggttaaaa tatttaaagc attgagtgat gaaactagac
    21781 ttaacatact tatattagta tccaagagaa atatatgtca aaaggggatt tctaaatatc
    21841 tagggatttc agattctgct gtttcacaac atataaaaat attaaaagat gttgggataa
    21901 taacaggata taaagaagga tactatgttt tatatcatat taataaagaa tcatttaata
    21961 tatgtgtaga ttttataaat tctatgttaa gtaattctag tgaaagcttt atagatgtat
    22021 ttgatgtaaa tacaatacat ctaggatgca gtaaagaatg taaatctata aaaaaatgtt
    22081 gtaagagaag ggagaaataa taatgaaagt atgtatacca gtagaggaga ataaaggatt
    22141 ggatagtaaa ccatatggac attttggttc tgctccaatt tttgttgtgt gtgatttaga
    22201 aagtggagaa gttaaatctt tagataatgg agatttagat catgaacatg gaaaatgtca
    22261 accattaaaa gcattgtcag ggacagctgt agatgcagta gttgttggag gaataggaca
    22321 aggagctata attaaattaa atggaatggg aataaaagtt tacagagcag aaggtgatac
    22381 tataagtgct aatttagact tacttaaaaa tggaaaatta gtagaatttc catcagacca
    22441 tacttgctca catgacggat gtggacatca ttaaaaaaaa acagctgatg aaatatatac
    22501 ttcatcagct attttttctt ttaataaata cgattttatt cttttaaaac caaaatttag
    22561 atttttatat tttttgtagt atccttaata atatttcaag tttacaagtt tactactttt
    22621 agattttttt tgtatgtttt tctatatagt caatatcatt taatatgtca ttgagttctt
    22681 tattaagtgt ttttaaatca tcaccatcta cattgtagtt taaaaatagt tcctctacat
    22741 tatcaatgat agaattgact tttctagtaa cttcaacaaa tgtcttaaag ttactactat
    22801 ttttagcatt tagaaatttt tttaaatcct ttgatataaa tgatactatt tcatattcat
    22861 cattaaatag acctctacca ataggatatg gttcaattct ctgtaaaaaa tattggtctt
    22921 tagcattaat attataagta taagatattt cccaaccgtc tcctaaatca aacttgataa
    22981 aatataatgt actacttact tttgatattc ttgtggcacc aagctgtctt aaaagaactt
    23041 caatcgaatc accttgaaca gtattaatat tcatatatac acatcccctt tagtattatt
    23101 atactatatt ttttaaaatt ttataattaa ataaacagta gatataataa tagatataat
    23161 cataagtgga aaacctattt tgaaatactc cttaaaagag ataggatgac cgtgtttatt
    23221 acctatacct gctagtacaa cattagcaga tgctcctatt agtgttccat ttcctccaag
    23281 acaagctcca agagatgttg cccaccatag tggcattaca tcaatacctt cagcttgcat
    23341 agtcaaaata agaggaatta gtgttgcaac aaatggaata ttatctaaga aagaagagac
    23401 tatagctgac aaccataata tcaacaacat tgtaaataca agatgtcctt ctgtaagtcc
    23461 aatcaatgct tgagccaatt tgtttattat cccaacttct actaatccac caactactac
    23521 aaataatccc ataaagaata aaattgtaga ccattcaata cctgccataa tttcatctac
    23581 atcttgtttt cctattaaaa gcattataga tgctcctgtc agtgctacaa cagaagaatc
    23641 tatgtggatt gtactgtgaa acataaaacc aaaaagtatg aatgctatta atataaggct
    23701 ttttataagt agaggtttat ctttaacaga ttttttttca tctagtttta atattgcatt
    23761 tttagctctt tcattcacaa caagttcttt tccataaata aatctaaagc atattatagt
    23821 tatagcaagt attactataa cagcaggacc taaatttatt acaaaatcca taaaactaag
    23881 attggcagca ctaccaatca taatatttgg agggtctcct ataagagtag ctgttcctcc
    23941 tatatttgaa gctaaaatct gtgttattaa aaatggaact ggattaagtc ctaatatttg
    24001 tgtaataact atagtcattg gtccaataag taagacggtt gtaacattat ctaatacagc
    24061 tgataaaata gctgttataa tagcaaagca aatcattatc ttccatgggt cacccttagc
    24121 tttctttgca gtccagatag ctatatattc aaataacccc gagtttttga caacagctac
    24181 aaatagcatc ataccaacta atacaccaat agtattgaaa tctatatggc ttaatccttc
    24241 atcaagtgta agtatattga atattatcat taatagtgca ccaccaacag ctgctacgga
    24301 tctgtttacc ttttctgtaa taatagctgc cataactatt aaaaatatac atatagctat
    24361 tatttgttga aattccataa tataagtacc tccatttgta ttattttgtg cctaatgcta
    24421 gtactatata tagtattact tttttattgt tttttcaatg gatgtatgaa agtatattat
    24481 ttaacaaagt ttttgaaaaa ttgacagaat ttaaagaaaa tttttgttat ttaaataaat
    24541 tttaaaatta ttccattgat aataatatta aacgacaaac gaaaaaatat accctttaaa
    24601 ctagaattta
//
LOCUS       DAMJWS010000065        24532 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMJWS010000065 DAMJWS010000000
VERSION     DAMJWS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.065"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            48..1925
                     /locus_tag="PC399_002613"
     CDS             48..1925
                     /locus_tag="PC399_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2593466.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTG
                     AYEGTINYNQDPEYLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTV
                     NASQTRLNGSNRYETSLLIAKEIDKNHDVEKIYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKDSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGAD
                     RHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     APGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPD
                     LFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRV
                     LPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSL
                     K"
     gene            2167..4080
                     /locus_tag="PC399_002614"
     CDS             2167..4080
                     /locus_tag="PC399_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593467.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKVTTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4073..6292
                     /locus_tag="PC399_002615"
     CDS             4073..6292
                     /locus_tag="PC399_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2593468.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKSGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLSLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6401..7231
                     /locus_tag="PC399_002616"
     CDS             6401..7231
                     /locus_tag="PC399_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2593469.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7221..10688
                     /gene="addB"
                     /locus_tag="PC399_002617"
     CDS             7221..10688
                     /gene="addB"
                     /locus_tag="PC399_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF2593470.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIINFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10688..14515
                     /gene="addA"
                     /locus_tag="PC399_002618"
     CDS             10688..14515
                     /gene="addA"
                     /locus_tag="PC399_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF2593471.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKSDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14561..15772
                     /locus_tag="PC399_002619"
     CDS             14561..15772
                     /locus_tag="PC399_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF2593472.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15759..19286
                     /locus_tag="PC399_002620"
     CDS             15759..19286
                     /locus_tag="PC399_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2593473.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEIPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19417..20670
                     /locus_tag="PC399_002621"
     CDS             19417..20670
                     /locus_tag="PC399_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2593474.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            20819..21871
                     /gene="ytvI"
                     /locus_tag="PC399_002622"
     CDS             20819..21871
                     /gene="ytvI"
                     /locus_tag="PC399_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2593475.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            21961..23187
                     /gene="pepT"
                     /locus_tag="PC399_002623"
     CDS             21961..23187
                     /gene="pepT"
                     /locus_tag="PC399_002623"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF2593476.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPKIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            23394..24446
                     /locus_tag="PC399_002624"
     CDS             23394..24446
                     /locus_tag="PC399_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF2593477.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADNISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT        10477 a         2350 c         4377 g         7328 t
ORIGIN      
        1 ctatggaaaa taacgctgat ttaaatataa acttagagaa aaaaagtatc gttttaggta
       61 gcaaatcaaa agttagtgtc aaatttaaag aaaaaccaga tgcagatagc attacattaa
      121 agtataaatg ctatgacatg ccattggata caactctaaa ttacaatcaa tcaactgggg
      181 catatgaagg aactatcaat tataaccaag acccagaata tctaaatgtt tgggaactac
      241 aagggataac aataaacagc aaaaataatc ctaaaacttt aaacagacaa gacctagaaa
      301 agctgggatt aaatttaaaa gactataatg taacacagga atgtataatt gaagacataa
      361 cttctagaaa agatgtaaat aaatatttga gaaaaacttc ttcacctatt acagaactta
      421 caggaagtga tagatatgaa acagcagtta aaataagtaa agagggttgg aaaaatggtt
      481 cagataaagt agttataata aatggggatg taagtataga tggcattata tcaactccac
      541 tggcaaccac atataatgct ccaatacttt tggttgaaaa aaacaatgtg cctaatagtg
      601 taaaatcaga attaaagcgc ctaaacccta aagatataat tataattgga gatgagaatg
      661 ctatttctaa aactactgct aatcaaatta aatcaactgt aaatgctagt caaacacgtt
      721 taaatggttc taatagatat gagacatctt tattgatagc aaaggaaata gataaaaatc
      781 atgatgtgga aaaaatatac ataacaaatg ctaatggcgg agaagtggat gcacttacta
      841 tagcagcaaa agcaggtcaa gacaagcaac caattatatt aactgataaa gatagtatta
      901 cagacaatac atataaatgg ttaaagagtg aggatttaca aaatgcttat tttataggtg
      961 gtcctcaaat gatatcaaca aatgttataa ataaggtaaa tggaataact aaggatagtg
     1021 ttactaataa tagagtatac ggagcagata gacacgaaac aaatgcaaac gtaataaaaa
     1081 aattctatac agatgatgag ttagaggctg ttttagtagc taaatcagat gtacttgttg
     1141 atgctttagc agcaggtcca ttggctgcga acttaaaatc tccaatactt ataacaccaa
     1201 agacgtatgt atctgcatac cataaagata atttagaagc taaatcagct aataaagtat
     1261 acaaaatagg aggaggattg acttctaagg taatgagctc tatagcatca tcattatcta
     1321 aacacaatac gactccaaca gaaccaggaa atagtggggg caagacagtt atgattgacc
     1381 cagggcatgg tggttcagca cctggaaatt catctggagg aatgattgaa aaagattaca
     1441 atttaaatac ttcacttgca acaactgaat atttacgttc aaagggattc aatgtaataa
     1501 tgacaagaga cacagataag actttatctc ttggaaatag aactgctcta tctaattcat
     1561 tgaaaccaga tttatttaca agtatacatt ataatggctc aactaataaa caaggtcatg
     1621 gtgtagaagt attttataag cttaaagata aaaatggagg gactactaaa actgtagcta
     1681 ccaatatatt aaatagaatt ttagagaaat ttaaacttac aaatagaggt ataaaaacaa
     1741 gagtacttcc tagtgattct acaaaagatt atttatacgt tttaagaagt aatgatatgc
     1801 cagctgtact tgtagaatgt gcatttttgg ataatgaaaa tgatatgagt ttaataaact
     1861 catctgcaaa agtaaaagaa atgggtacac aaataggtaa aggaatagaa gattcattaa
     1921 aataaatgga aataaaaata tttaaaaact taatgtaaat ttatagttgt aaatttatac
     1981 taaagtcaaa aaatgaaata taagtgtatc aatagtaaga ctgtatgtac atttgcacat
     2041 gtgaatgcaa gtatatatac agtctttttt tgtagttaaa tgcaataatt tgattgtgtt
     2101 ttgtaaaata cacaatatga tataattaga taatatacac ttaatttaat tgaggaaggt
     2161 gattatatgg aatatttatt tgaaaaaagt agtttaaaag aatatggaaa tgaagcttta
     2221 gataaatata gagaattatt agataaaact ggtaataatt caaggtgtat tcttttgtta
     2281 gtaccaaaca ataacacaaa gatacgatat gaaagggctc taagattaaa ttatagtgaa
     2341 gaactgaaag taacaacata tataagtttt gtgaagaaag aattagttaa atactggcca
     2401 ctaataatag aaaaatgtga tggaataagt acaaaggttg tatctccaac atttatatca
     2461 aatagtttaa gtgaatattt aatagtgcaa aaagttaagg aaaaaaggat acaagaagga
     2521 tattttgaag atattacagg aaccaataaa tccatagcaa atagtataat gactaacata
     2581 aataagtctg catttaattt aattgatttt gattttattg gagaaaaaat atattcatca
     2641 aagaaaaata aagatagtat atatagattt tcatacactc aaatggatga aattatatca
     2701 tattatataa attccttact atcaaattca attttagata atgcattaag catatattta
     2761 tacaatcaat acctacttaa tgatgatttt tatttaaaaa acttatttac agaagtgaga
     2821 tatataattg tggatagcct tgaaagctca agtaatgcag aagttgattt tataacagaa
     2881 gcattggata atacattaca aagttatata tattttgatt atagtaggga ttattctgta
     2941 tttaataatg tggatataga ctatataaat gagaaaataa tatcaaaaat taagcctaaa
     3001 gaagaaaata ttaaaaagaa ggagattaga atagaagatt tatacttatt acctgcaaat
     3061 atagaattaa atcaatcaag tcaattatat aatgaaatgt tagatttaat aagtgaaaaa
     3121 gttatatctc ttatagaaga agatgtatct ccaagagata tagcaattat atctcctata
     3181 aacaatagta tattggagca tcaaattaga gattctttga tagaaaaaaa tatagatgtt
     3241 tttagtacta agaaggataa aaaagctgtg gattatccat atgggaatgc attggtagtg
     3301 gctacatgta tattctatgg atatttagat tttataaaag atgatgaata cttaagcttt
     3361 ttagaaactc ttttagaaat aaataggata aaagcattta agatttttaa tgaaacaaga
     3421 catttaagta ttgataatat agatgaacat agtcagtatc gtgatatttt acagtatatt
     3481 gaagaaaaaa agaagagtga tataaaaata catgaatttt taactcaatt ttacatagat
     3541 aaaatgttaa atttaaaaga aggtaaaaag aatgttgggt tatgtaaaaa gataatagct
     3601 gaaagtgagt cttttagtga aagtttaagt ctacttggga tgaaaaaaga aaagatattt
     3661 gtagaagctt taaaaagtac aataaacgat tattactctg ttgtcgatat agaagagttg
     3721 aaatccaaag acaaaatagt aataactact ccttattcat atatatcatc aaatatagat
     3781 agaagtatac aaatatgggt agatatagga agtaatgctt ggaatatgaa aatagaaaaa
     3841 gatataagta atttaattgt acttagaaaa tcttttgaag aaaaaaagat ttatacaaat
     3901 gaaatggaag aatattataa aaaatattat ctatacaata cagtatataa tttgttatta
     3961 aatgcaaaga aaatctatgc atttaaaagt gaatatgcga ttaatggtta tatccaagag
     4021 agtatattat atagtatact tttaaaaatc tcacataaag gagataaaaa ttatgattaa
     4081 tataaattat agagaagacc agataccaat aataaattac gataatggta ctatggcagt
     4141 accagcagta ccaggtgctg gaaaaacatt tataataaca aatttagtag ctaaactatt
     4201 acttgaacag aaacataaga gtggtaaaat attaattctt acatatatga atagtgctgt
     4261 aaataatttt aaaggtagaa taagaaagat tcttgaagag aataaaatag atgatactaa
     4321 ttcgtatgaa gtaatgacta tacacagttt ggctgtaaaa ataataaagg aaaagccaga
     4381 agttgtaatg ttaagtgagg attttaacat agctgacgat ttgcaaaaaa gtattatatt
     4441 aaatgaatgt ataagtagat ttagatttga aggtggagaa agagctttta gatggttttt
     4501 aaaggagcaa aaagatgaac gctggaaaga aataactctt gaagcctggg aaagaggttt
     4561 ttttgaattt gcaggaaatg caataagtga acttaagtat aagggaattt ctccagataa
     4621 acttgaagaa atattgtcaa gaggacacaa aggaatgctt aaaattattt taccaatata
     4681 taaattgtat gatagaaaat taaagcaaaa tggattgcta gattatgatg atatattaat
     4741 tttagcagaa aaaactttaa gcttagacga agggcttaga aagaagtttc aaactagata
     4801 caaatatata tttgaagatg agtgtcagga ctcaaatgaa attcaaggta atataataaa
     4861 aattatatct agcgaaaata aaaacctagt tagagttgga gatataaatc agagtataac
     4921 aggaacattt tcatcatcag accctaaatt ttttaaagaa tttataaata gtgcagattt
     4981 ctgttatagg atggatatgt caaatcgaag ttcaaaagat atacttgatt tagcaaacac
     5041 tcttgtcaag tatgttacaa aggaatttag acaagaagaa tgtagacatg ctcttgaaga
     5101 tatgcaaata aaaactgtgc caaatggaat gggatataaa gaaaacccta atccagaaca
     5161 ttataatata aatgtaaagt ggtatgagag ttggaaaatt gaaatagagc aaactgctag
     5221 atacgttaag ggtataaaaa ataaatatcc agataaaagt ataggtatat tggttccatt
     5281 taatgaacaa gtgacacaag ttgctaaaga acttagagag aataatctaa cctttgaaga
     5341 attaggacca aattctttga ataaaaggaa ggttataaac aacatagcat atattattga
     5401 ttttatgtta cattgtgatg atgtagaaaa gttaatagtt gtacttaata aagtttttat
     5461 agatactgat aatgaagaag aaataaagta ttttttagaa gaattaaaaa actattctac
     5521 cgaagaaatt atttataatt ttgaatttag taagttagat aatgatttaa tagaatttaa
     5581 agaagaaagt gatatatact taagttttaa aagaggaata gatattataa aacagatatt
     5641 agaatatcca ataataaggt tagacctctt agttttgttt attgggaaaa aacttagttt
     5701 agaaaaagaa gataaagctg tactggatta tatatctttt tatataaagt atctagtatc
     5761 agaaaatata aatatggatt tagaagatgt atataattta ttgtttgatg taaagaacag
     5821 ggtgtttaat catataatag atgtggtata tgagataaat ggatatgaac cagagcctgg
     5881 tagtataaca ctatgtaact atcataaatc aaaggggatg gaatgggatt gtgtgttttt
     5941 actaggtctt gttgaatata atttccctga caatattaat caaaagtttc agagtgataa
     6001 atggtatcta aaagaaaaat ataaaaaccc tatggcaatt attaaatcag aagttgaagc
     6061 tatattaaaa ggaagtattt caacagatta tgctcataaa acgaagattg attctataaa
     6121 tgaaaagatt agacttttat atgtagggat tactagagca aaggaaatgt tagtactttc
     6181 aggaagtgct tatagagatg aaagtgatat agggaataag agaaaagagc aaaaaccatg
     6241 tatatattta agcagactta atcaacatat tattgaaaaa aggtcaaatt aatatataat
     6301 aagttattaa aagattgttt gaaatatatc taaaagagga taaaacaata tgtacagtaa
     6361 gtaactacta aaaaataaag ttaagagaga agtgatatga gtgaacgata aattaaaata
     6421 tttttcttat agtcaaaatt caattaacac atataaatca tgtccattga aatttaaata
     6481 taaatatatt gatagaataa attggaaaaa tgatgatgtt ggtagtagag agtattatga
     6541 aactttaaaa acaggaagag attttcattt gatttgtgaa agatatttta gcaatatacc
     6601 tcttggaatc tattttaatg aggatgataa aaatagtaaa aagtttttaa agtggatgga
     6661 aaatataaaa aaagttgttc ctattgaaaa aggaaaaacg tatttgccag aatatgaagt
     6721 aaggatgaca ttaaatgggg atataataca ggcaaagtat gatttagttg taattggaaa
     6781 taacaatata gaaatatggg attggaaaac cgagtctaaa aagattgact ataaacacgt
     6841 agaaaataga atacaaacta ttgtttatat gtttttagca aaagaagtaa tacctaaagt
     6901 tttaaaaatg gacataaatg ttaaagatat aaatatgaga tattatcaac ctgaatttga
     6961 tgacttacca attaccattt tatatgatga agaaaaacat gaagcaaata gaaataaaat
     7021 tcaaaattat ataagtatga taaaaaacac taattatgaa gaacatacat acgaagacaa
     7081 tctatacaat gatatagaaa aggtatataa taaagaagaa cgtatgtgct atagaaataa
     7141 aaagcattgc aagtattgtg aatttaataa gttatgtaat ggcaaagaaa tagattatag
     7201 tattttggag gcggaaattt atgggactta ggtttgtatt aggtagaagt ggaagtggaa
     7261 aatccaccta tatactagat gagattaaaa aagaagctca aaaaaatgaa acgacatcaa
     7321 ttatactttt agttccagaa cagtacacat ttgaggctga aaatagagta agtaaactat
     7381 ttttaggaaa agaaaaagat aagtatctaa gagttagggt acttagcttt aagacactaa
     7441 gtaatatagt tttttcacaa gtaggaggat tgacagatgt aaatataaat tctagtggaa
     7501 aagcaatgat ggtatataga gctattgaag atgtaagtga agaattaaat gtattttcaa
     7561 aatcaaaatc gcagtctggt tttgtaagct caattacaga tatgatatca gaaatgaaac
     7621 aatacaatat aagccctgaa atgttagaaa atatatctgg agaattggat aatgaaactt
     7681 taagtttaaa attaaaagat ataagtaaaa tatataattc ttttgaaggg aaattgcatg
     7741 aaaattatgt agatgctcaa gatatgttaa cttcattggc aagtaaaatt gaattaagca
     7801 gttatttaga tggagcttgt gtttatatag atgaatttac aggttttaca ccaaatcagt
     7861 acaatgtcat aaagagtata ttaaataagt ctaaaagtgt taatatctct ttgactgtag
     7921 atgatatcaa ctatattggg tacagtaaat ctgatatgtt ttccagaacg aaatttactt
     7981 attcaaaatt gactcaattg tgcaacgaag aaggcataaa aatattgcca caagtaaatt
     8041 taaacacagg tgttataaaa agatttgaaa aagttaagga attacagcac ttagaaagat
     8101 tttataatgc ttatccatat aaaatatatt caaatcaaac tgaaaatata aagataaaag
     8161 agtttaacaa tttatattca gaggtggaag agatagccag agaaattgta catttagtta
     8221 gagataagaa tgtcagatat agagatataa ccattgctac aagagattta aatagatatg
     8281 attttttagt acactctata tttaatgaat ataatatacc aaactttata gataagaaga
     8341 gagaggcaaa aagtaatcca atagttattt taattatttc agctttagaa atgaagaata
     8401 gacgatatgg gtatgaaact atgtttaggt atttaaaaag tggacttata ggaattgata
     8461 atgatgatat aaatttacta gaaaattatg tgttggctaa tgggattaaa ggtaaaaaat
     8521 ggtttgatga aaaatgggat tatagaataa cccaaagtct ttcaggtcaa gaaagtgaat
     8581 ttgaactgga attaaaagag aaaataaatg aaataaaaaa tagagtatta gagcctatag
     8641 taattttaca agaaaaatta agaggaaaaa atagagtaaa agaaatctgt agatatatat
     8701 atgaattttt attagatata aatatgccag aaactataga aagtttaata ataaatttta
     8761 aggataaagg ggaacttgat gttgcaaatc aatactcaca agtttgggac atagtagtgg
     8821 acatattaga ccaaatggtg gaacttatgg gtgatgaaat aatttcttta gaaaaattta
     8881 taaaattaat aaccttaggt tttgatgaat atgaattagg actggttcct cctagtatag
     8941 accaggtact tgtaagtagt gtggatagaa tgaaaaatcc agacacgaaa tatttatacc
     9001 tagttggaac tactgatggt gtgtttccgc tgattacaaa ggatagtgga atattgagtg
     9061 ataatgatag ggaaagtctt ggaaacaaag gaatcgaagt tgatattgat agtaagacta
     9121 ggagttttga agaacagttt cttgtatata aagctcttac ttcgacatct aagaatttaa
     9181 ccataacata tccaatatca gaccatgaag gtaaaacgct tagaccatca ataattatat
     9241 caagacttaa gaagatattt ccaaatatag aaaataaaag ttatttagtg gaagaaaata
     9301 aaagcactga taaagatata cttaaaaaaa taacggttaa atcccctaca tttaatgaac
     9361 ttataaacgt aataaaaaat tatgacagtg atggttacaa tacagaagag attaatagta
     9421 tttggttaga tatatatagg tactatttaa aagatgaaat ctacagttct ataacaaaaa
     9481 aagttataaa aggtcttagt tatactaatc aagttcataa gattgaagag aaaaaaataa
     9541 ggtcattgta taaaagtaat tctcttagca tatctaggct tgaaaaatat gctgaatgtc
     9601 catttgctta ttttatacaa tatggtctaa aagcaaaaaa gcgtaaagag tattcattta
     9661 caccaccaga tttaggaact tttattcata atattttaga taggttttca aaagaacttt
     9721 tacaagataa tttaacttgg agagatattg atgaaaaata tatagaatta aagataggta
     9781 taatagttga tgaaataatt ttaaagatac caggatatat attaaatagt tctgagaggt
     9841 ataaatacct tgcatataga ttaaagaata tgttgacaac tgctataact ataattagtc
     9901 agcaaataaa acaaggttca tttgaaccaa tagattatga agttaaattt ggcaacaatg
     9961 gaaaatatcc acctataaaa atggtgttag aaaatggaca agaagtaagt ttaattggac
    10021 aaatagatag agttgatgaa tttgaagaag gagaaaataa atatataaga ataatagatt
    10081 ataagtcagg caataagtcc ataagtctaa cagaaatata ttatggtcta caattacaat
    10141 tacttgtgta tttagatgca atccttgaaa gtgcaaagga tgaagatata aatataaatc
    10201 cagctgcaat actatattgt agaataaata atccaatagc aaaatttaat gaagataaag
    10261 atgatgaaga aatacaagaa gcaatattaa aagaacttag aatgaagggg cttgtagtaa
    10321 aagactctca catagttaaa gagatggata aatcattaat agatggagaa aggaaaaact
    10381 ctttagtaat accagtaggt cttactaagg atggaaatgt aggtaaaagt acttcagcaa
    10441 taagctatga ggatttcaaa ctgcttagaa aatatgtaag gcatgctata aaggatttat
    10501 gtgaagaaat gttaagtgga gaaatacgta tagctccata taaacataaa gatggaacat
    10561 catgtgactt ttgtgattac tctgctatat gtcagtttga ttcaactata aaagataaca
    10621 agtataaaaa tttaaataat aagagtaatg aagagataat aaaaatgatg aaaggagatg
    10681 ttaattagtg agttctccta aatggacaaa agagcaatta gaagttatag aaagtagaga
    10741 atgcaattta ttggttgctg ctgctgctgg ttcaggaaaa acagccgttc tagttgagcg
    10801 tataattcaa atgataacaa gtagagaaaa tccaattgat atagataagt tattagttgt
    10861 tacatttact aatgctgctg cgtcagaaat gagagagagg ataggggatg ctataggaaa
    10921 agccttagat gaaaacccag aaaataaaca tttacaaaac caacttgttt tattaaataa
    10981 atctagtatt acaacaatac actcattctg tttggatgtt ataaaatcaa actttcatag
    11041 aataaactta gaccctaatt tcagaatagg agaccaaact gaatgtgcta ttttgaaaca
    11101 agaagcaata gaggaagtat ttgaagattt atatgaagaa agagatgagg ggtttttaaa
    11161 tttagttgaa agttatgcag aaagaggagg agataaagaa gtacaagata ttatcttggg
    11221 aatatattca tttgcaatgg cttctcctga acctaaaaaa tggttgattg actcagcaga
    11281 acgatttaat attgatgaaa attttgattt ttcacagtct atttgggcta gagctatatt
    11341 agatactgta aaaatagaga ttaatggtct ttgtctaaat atggaaaggg cattaaaaga
    11401 agtggaatca atagaagaat tagaaacttt tgcagaaaag ttatccgttg aatataaaaa
    11461 aattgctgac atttcacaag catgtaataa atcttgggat gaggcatata aaaagatggc
    11521 aagtatgtcc tttgaaaatt atgtaaaagg agtaaaaaga atttcaaaag atgctccttc
    11581 gtatataaaa gaatcaaaag aaaaggcaaa aactataaga gataagacta agaagtcttt
    11641 agaaagtata gtaagtgcta cattcaataa agataatgat tctattagag aagaaataaa
    11701 atatctttat aacatagtta aaccaatatc tagtgttgta ctgagatttg aagaggaata
    11761 tagcaataag aaaagagaaa aaggaataat agattttaac gatatagagc attttgcatt
    11821 aaatatactt acagatgtag atgaaaaagg taatattgta ccttctgata tagcagttgg
    11881 atatagaaac aagttttatg aaatatttat agatgaatat caggatagta atctagtgca
    11941 ggaagtactg cttaaagctg ttgcaaatac agaaactcca aatagattta tggttggtga
    12001 tgtaaaacag agtatatata gatttagaca agcaaagcca gaattatttt tgcaaaaata
    12061 taataactat aatgataaaa agggaagctc ccatagaaaa ataatgcttt acaaaaactt
    12121 tagaagtaga gaagaagttg tagatgctgt aaattatata tttgaaaata taatgaatga
    12181 gaatatagga gaaattgaat atacagaaaa agaaagacta aatttaggtg caaattttaa
    12241 tgtggataca gacgagaaat ctattattgg tggagcaacg gagatacacc taatacaaaa
    12301 agataataaa cttgatgatg atattataaa tgataaagac gatagaatta ataacaaaga
    12361 aaatgaaata gaggaagaag aaaaattaga taatatacaa cttgaggcta gaatggttgg
    12421 aaacattatc aaagatttaa tgaaagtcaa tgaagatgga aaaattcaaa aggtctatga
    12481 taagggaata gatggatata gacctgtgga atttagagat atagttattc ttttaagagc
    12541 tacatctgca tgggctccag tgtttgcaga tgaattgatg aatatggata taccaacgta
    12601 tgctgatgtt ggagtggggt attttgatac gattgaaata aaaacaatat tgtcattact
    12661 tcaaataata gataatccaa tgcaagatat acctcttata tctgtattaa aatcacctat
    12721 atttggattt acaccagagg atttgattga tattagagtt cagagtaaag ataaaatttt
    12781 ttatgaggtt ttaaaaagta cagcagagta tgatggattt acagattctc aaaatgaaaa
    12841 tgagtctgag tttataccaa gtgaagaatg cataaacaaa agtaaagatt ttttaattaa
    12901 gctaaaggaa tttaaagaaa agtcaatgta catgagtaca gatgaattta tatggtactt
    12961 gtacacaagg actggatact atgcctatgt tggagcattg ccaggtggaa gtcaaagaca
    13021 agctaattta aaagtattgt ttgagagagc aaaacaattt gaagaaacca gtcttaaagg
    13081 aatattcaac tttgtaaatt ttatagagaa attaaaaaaa tctagttctg atatgggaag
    13141 tgcaaaaaca cttggtgaga atgcaaatgt tgttagaata atgagtattc ataaaagtaa
    13201 aggtctagaa tttccagtag tcatatgctc tgcaatgggt aaaaatttca atactcaaga
    13261 ttttaaaaag agtattttat atcatcataa tttagggtat ggacctcaat ttgtagacta
    13321 tgagagaagg atttcttttc caagtatagc caaagaagca ttaaaaagca aaataaatat
    13381 agaaaattta tcagaagaga tgagagtttt atatgttgca tttacaagag ctaaagaaaa
    13441 attgattata actggttcta ctagaaatat acaagatagt ataaagagat ggtcaaatgg
    13501 aattgaaagt ttagatacaa tatcacaata cgagatatta aaaggtaaaa attttttaga
    13561 ttggataatg ccttgtgtat taagacatag ggatttatct aatctactag aagaagtagg
    13621 attagacgca gtttttaatg ttgaacataa ttcaaaatgg tatggaaagt tgtggaataa
    13681 gagtgatatt ttagtggaaa agaagagtga tgaagaaaaa gaaagtattg aagaaattct
    13741 tgaaaaaata gatgtagata atcctgatag tgattattac agtgaaatag aagaaaaatt
    13801 aaactatata tatccatatg aatttagtac aagaaaacct gctactatat ctgttacaga
    13861 aataaaaaag atacaaaata attatgaaga agagttaata aatactatat ttgaacaaaa
    13921 agtaatatta aaaaagcctc tatttattca aaatgaagaa gaaagagaaa aaataagtgg
    13981 aacagaaaga ggtacaatag tccatttagt tatggaggtt ttagacttaa aaaatgtaag
    14041 tagtgtaaat gatataaaat cacaaataag aggttttgtt tcaaaaggta taataacaga
    14101 aaaacaagct agtatagtaa atccatataa aatatataaa ttttttgcct ctaacatagg
    14161 aaaaagaatg ttgaatgctg aaattataaa tagagaaaaa tcaatttatg cacaagtaaa
    14221 tatgaaagac atatatattt atgaaaagct tataaacaat gacgataaaa aactttatga
    14281 taatgaaagt gtcatgttaa gaggtatagt tgatgcttat tttgaagaag acaatcaaat
    14341 agttttggtg gattataaaa cagattttgt aaatgaggaa aatataaatc aaataataga
    14401 gaaatataaa aaacagttgg atttatatgc agatattatt gaaactttaa ctggtaagtc
    14461 agtaaaagag aaatgtatat atttatttgg agttgatgaa gctgtttgtt attagttttt
    14521 atatataaat taaacctaaa agatagagga aggggaaaaa atgagattta ttcatacatc
    14581 agattggcat ctagggaagt ccctagaagg ccattctaga attgaggaac aagcaaagtt
    14641 ttgtgaggaa tttataaaaa tagtagaatc aaatgaaata gatatggtta taattgctgg
    14701 agatgtatat gatacttcta atccaccagc acaagcagaa aaactatttt atcagactgt
    14761 atcaagactt gccaataatg gtcagaggtg tgtattaata atttcaggaa atcatgataa
    14821 tccagagaga ctctctgcaa taacacctct agcacatgaa caggggatac ttatatatgg
    14881 ttatccatta agtgctacga tagaagctaa gtataaaggg tttgaaataa catatgcaac
    14941 tcagggatgt acaaaactga atataaatgg agaaaatatt gtggttgcta cattacctta
    15001 tccaagtgaa aaaagattaa atgaagtttt ttcaagtgaa gatgagtttg agaaacaaaa
    15061 aaattattct gaaaaggtag gagatatatt tagaagttta gaagaaaatt ttagaagtga
    15121 tactataaat atagccgtat cacatatatt tgtaataggt ggagagagta ctgaatcaga
    15181 aagacctata caattaggtg gaagtttttt agttgaaaga aaagatttgc cagaaaaggc
    15241 tcaatatact gcactaggtc atttacataa acaacaaaaa gcttccgaac gtttaaatgc
    15301 atactattct ggttctccac ttcaatatag caaggatgaa agagcgtata ccaaaggggc
    15361 atacatagtg gatataaaag ctggtgaaaa accaataatt gaagatgtat attttaataa
    15421 ttacaaacct atagaagtat ttaaatgtaa tggaatagaa gaagcactag atatatgtga
    15481 gcaaaatcaa gatagagaga tttggtcata ttttgaaata aatactgatg agataatatc
    15541 acagaatgaa attaaaaaaa tgaaggaact attaaaagac ataatagaaa taaaaccaat
    15601 aataacatct tgttatgaac aagaaagtgt agatataaaa gaaaagtcta tggcagagtt
    15661 gtttagagag ttctattctt ttagtaaagg tgtagagcca aaaggagaac ttatggattt
    15721 atttctagat ataataagtg aagaaggtga atcagcagat gagaccaatt agattagaac
    15781 ttacagggct taatagctat attgataaac aagttataga ctttgaaaaa ttaattgaaa
    15841 gaggtttgtt tgggatattt ggaactactg gaagtggaaa atctacaatc ttagatgcaa
    15901 taactattgc tatgtatgga aatatatcca gaaatacaaa agaatatata aacagtgtgt
    15961 gcgataaggc aattatatca tacgagtttg agataggaag caaaaacact agaagaagat
    16021 acattgtaga tagaaccatt gctagaagca aaacaggaac aaagacctct aatgctagat
    16081 taatagaagt attgaatgac aatacccaaa atgttttagc tgataaagtt gtggaagtaa
    16141 atgaaaaagt agcgcaggtc gtaggattaa cggcaaatga ttttactagg tcagtagttt
    16201 taccacaaga caaatttaat gagtttctaa gattatcacg tgcagataga agagacatgt
    16261 tagagagaat ttttaatctt gagaaatatg gaagaagtct tggcgaaaaa gtgaaaaaga
    16321 gaaaaaatat acagttacaa aatttaaaag atttgaaatc aaaattaagt caatatgatg
    16381 gaattacaga ggaagtatat aacaatataa accaagaatt aatagagtta aaaaatttgg
    16441 aaaaagataa gaataatgct ttagacctag ctcaaaaatc ttatgaagaa agtaaaacag
    16501 tatatgaaga acaattaaag ttagaaaaaa atgaacttag aaaaaatgag cttgatttaa
    16561 aaaatagtga gataaaagaa aagattgctc ttgtagaaaa tgatagtaac gctaaaaagg
    16621 tagacccata cataagttca gttcaaaatc tagaaaaaaa gatagatgaa gatagcttcg
    16681 cagtaagtaa tttagaaaag aaattagcaa tattaaatca ggagcttgaa gttacaaaaa
    16741 atagatatga aaaaataaac aaaattaaga atgaagaaat ccctaaattg agtgaagaaa
    16801 aaataagatt gcaacaagca ataaaattag aagaagagtt agttttatta gatagagagt
    16861 taaaagagct aaaagaaagt ggaattaatt taaataagac taaagttgaa ttagaaaaag
    16921 taaagcaggt gtcagagtcc agaaaagatg cagttactaa atctataaaa gaagttgaag
    16981 gaaagattga taaagtaaat ataagtgcag aattaaaaca aaaaatattt ctagcatatg
    17041 aatacgagaa agattacaac aacgtattgg aagaaaaaaa tcaaaagcta aataaactag
    17101 aagaaatact caaagagaca gaaaatataa atttaaaagt tagatatatt gataaagata
    17161 agaatgatgt aaatcgtaat ttagaaaatt tatcacttca cttggatgtt cttcttaaaa
    17221 aatgtcctgg taagagtgct gatttacttt taaaatctga atatgttact gaattaagaa
    17281 ataaagcaaa taatactaaa gaaaatgaaa ttaagaaaag cagtatacaa gatgaattaa
    17341 agataatact tgaaagtaag tttaatactg aaagggaagt aaatttacta aatgaaaaac
    17401 ttgaaaataa tagaaagaac agagatgact tagaaaaaga attagaagaa ctaaagtatt
    17461 taaatttagc ctctgaactt agacgtgaat taaaagaaaa tatgccttgt ccagtttgtg
    17521 ggtcaaaaca tcatgaaaat catgatataa ctaagtatga tgaaaatata tcttttgtca
    17581 aagaaaaact ggagaaatta gaaaaagaaa aaataagtat aagaaacaat atagaggaat
    17641 taaatgctaa agtaagtggg tatttatcta tagaaaaaat gaagactaaa gaacttgaag
    17701 atgtaaaagg aaaactaggg gggattccat ctagtcaatt attaaaaaaa cttgatgaag
    17761 aacaaagaaa acttgcattg ttaaaatcta atattcagga gtgggagaaa gaaaaagaga
    17821 gtactgaaaa taaagtaaca ttagctaaag aagaaaaaaa taaaattgaa aaagaagaat
    17881 taaaaataag agagagtctg aataattata agaaattaac aagagattta aacatagaaa
    17941 tagaaggttt agaaaataaa tgtaaaaaac ttaaacagga atacctagga ttaaagacga
    18001 ttactaaagt ctctaatcta ttgtcaaaag taaaagaaat aagggagaat gaaaaagaat
    18061 tagaattact aagtactggg cattcaaatt tattaaaaaa tagagactca ctagatatta
    18121 aaataagaga aaatgagagt aaactacatg aaatagagat agaattaata aaggctagag
    18181 aattatatgt agaaaagaaa ttgagtagag ataataagta taaagaagtt attagcataa
    18241 cgaaaggtga tttagctaaa aatcttttac ataatgttga agaaaatata tgtaagatat
    18301 tagaacaaga agaatcaagt aagaaaaaat tggaagagca aagattagaa tatgaaaaaa
    18361 acattgctga aaagcacaat atagatggta gacttaaaac tgctaaggaa caatataaag
    18421 accaaaaatc aacactaaat aaactattgg cagaaaataa atttgaaagt atatatgcag
    18481 taaaaagagc tttattagat actgatgcag taaaaaaatt gattgaagaa atactagagc
    18541 atgaagaaga acaaaagtta ttatcattta aaataaaatc atcaaaagaa aaattaaatg
    18601 gaagaagcat aaagaaggag tactttgaac agttaaaaga tgaaatttat aatttgaaag
    18661 tagaaatagg aaatatatcc aaagatattg gagctaatca aaatcagtta ataacattaa
    18721 aagactcttt agataagata aatgatttta ataaacaact taaggtagtt gaacacaatg
    18781 tggacttatt ggaagaatta gataaatgtg tacaaggaaa taaatttgta gaatatgttt
    18841 ctacaaacca attaaaatat atagcgttgg aagcatcaaa acgattggat ggaataacaa
    18901 aaggaagata tgcacttgaa attgactcaa cactaaactt tgtaatgaga gataacttta
    18961 atggaggaga aagaagaagc attgatactc tatctggtgg agagacattc ttgacatctc
    19021 tatcattagc acttgctctt tcatctcaaa tacaattaaa gggaagtgca ccattagaat
    19081 ttttcttttt agatgaaggt tttggttcat tagatagtga acttttagaa attgttatag
    19141 aatccttgga aagattgcat agtaataatc taagtgtggg tataataagt catgtcgagg
    19201 aacttaaaaa tagagttcct gttaagctat tagtttcatc tagtgaggct ggaataggtt
    19261 caaaagtaaa aattgaatat agttagaaaa tatatgttaa ttgaatatat tatctaatat
    19321 ttccaaataa tatgtaattg tgatataata aaaactaata ttaaagttat ttataattaa
    19381 tattaatata aaaacatgta agtaggagtg tggtaagtgt tggaatcgcc caataatttg
    19441 attcagatta tttttttaat agtattactt atagggtctg catttttctc agcatctgaa
    19501 acggccttaa tgtcgttgag taaaatcaga atcagatata tgcaagatga aggagttaaa
    19561 ggagctaagt tagtaagttc attaatagaa aatccaaata agcttttaag ttctatattg
    19621 gttggaaata acgtcgtaaa tatagcagca acatccatat caacatcttt gtttatagga
    19681 ttaatgggag aaaaaggagt ggcacttgct acagctgtta tgacagtatt agtattaata
    19741 tttggtgaga ttactccaaa aacaatagca gcaaataatt cagaaaaggt ttctctatta
    19801 gtatctaaac ctataaaagc aattatattt atattaagac caatagtatg gatatttaat
    19861 ataattacaa atattatatt taaattattt ggtataacaa ataaaggagc taaatcattc
    19921 ataactgaag aagagctaaa aactatggta aatgtaagtc atgaagaagg cgtccttgaa
    19981 atggaagaga gagaaatcat aaataatgtt tttgaatttg gagatatgca agctaaaaat
    20041 gcaatggtac aaagaataga tatggtagct atagatatgg aagatagtta tgatgagata
    20101 atacaggtat ttaaaactga aaaattaagt agaatgcctg tgtatgaaga aactatagat
    20161 gatattgtag gaatacttaa tataaaagat attatatttt tatctgatga agaaatagag
    20221 tcatttgata ttaaaaatta tatgagagaa ccttttttca cgtatgagtt caaaaagata
    20281 acacaacttc ttgaagagat gaaacttgaa aaaagtcaga tggctatagt tgtagatgag
    20341 tatggtggaa catctggact tttaactata gaagatttag tagaagtcat tgttggagat
    20401 atagaggatg aatatgatga agaagaggat gaaatacaag ttataaaaga agatgagtat
    20461 attgtagatg gaagtactaa aattggtgat gttaatgaac ttattggtgt aaacttggaa
    20521 tctgaggagt ttgattccat aggaggtttt ataattggac acctaagcag attgccagaa
    20581 gaaaatgaag taattgaagt tgataatata agattttgta tagaaagtat agaaaaaaat
    20641 agaattaaaa aaataagaat atatacataa tgtaataatg ttcttaaatt tattagaagt
    20701 gtataaatat aaaagatatc ttttatattt atacacttct ttttattttg tattcaaata
    20761 aaactagtat gaaatggaca aaataaaaat ataaattaga agaaaggaga tttttaagat
    20821 gagcatacta gaaaataatt ttttatacaa attaaaaaac aacactatct ttttttgtat
    20881 atatacattg ttatttattt ttatttacaa agcgtttcca tacatagctc cattttttct
    20941 aggtgctata atagcactta tgattaatcc tatatcacaa aagttagaaa ataagtttca
    21001 tataaataaa ggtatctcta ctcttgttct aagttttctt gcagtggcta tagtcagtac
    21061 agttactaca ataatagtaa taaattcaat gaaagaatta atgggatttt taaataatat
    21121 atctgctaat cctgaggata taagtaatac tattatgtat tttttaaata aaataaatga
    21181 ctttatgaaa tcatttcaag aaatagccaa ttttgatgta gaacaattag taaacaaatt
    21241 cagtggtgaa gtaatgcaaa ttacaaaaaa tcttcttact agtatacttg gattggctac
    21301 ttctattcca tatattataa tatttataat taccttgttt attgctacgt attttatagc
    21361 aaaagacctt gataagattg aaaatagttt ttataatatg tttactgttg atgttagaaa
    21421 gaaggtaaaa aacgtaaaga aagaggcagg gctttcactt gttggatata taagggccta
    21481 tacaatactt atggctataa cattttttgc aatctgggga agttttgctt tatttggatt
    21541 gaaatacggt ctaatagtag ggtttgtagg agctttaatg gatttaattc cattcctagg
    21601 tataataaca atatatcttc cagtaattgt ttattatttt cttataaaaa attattttat
    21661 agctataagt atgactgtga tattttttgt tttgtctttg attagagaaa tacttgaacc
    21721 taaactagta tcagtaaatg ttggactaaa tccattagct accctagcag cgatttttat
    21781 tggaatccaa gtaaaaggaa taattggagt aattttttgt ttgggtttgg tatgtatgca
    21841 tgatatactt aagaaggtgg atatttttta attataataa ttgatagatt ttttaaaaat
    21901 aaaattttat tcacaggtaa ataaaaaaat attaatctta ttaagaaaga gggtatatac
    21961 gtgaaacaaa aagtagtgga aagattttta aaatatgtat catttgatac aacatctaat
    22021 tctcagtgtg agaactgtcc atcttcagaa ggtcaaagag ttttagctaa gtatatagtt
    22081 gaagagctaa aaacaatggg tgttgatgat gtgagtttag atgaaaatag ttatataatg
    22141 gctactttaa aaggaaatac tgatggtgtg gatactatag gattcatatc acatttagat
    22201 acaattgaag atgttagtgg aaaagatatt aaaccaaaaa taatagaaaa ttatgacgga
    22261 aaagacatag ttttaaatga agcgttaaat gttattacgt atgtaaagga ttctccagag
    22321 ttggaagaat ttaaaggtga cgatttaata gtcactgatg gaacaactct acttgggtct
    22381 gatgataaag ctggtatagc tgagatagtg actgcaatag agtatctaat aaatcatcca
    22441 gaaattaaac atggagatat aaaaatagga tttacaccag atgaagaaat tggtagaggt
    22501 gctgatttat ttgatgttga aaaatttgga gcaaaatatg catatacttt agatggagga
    22561 atagttggag aacttgaatg tgaaaatttt aatgctgcaa atgcaactat tactatacat
    22621 ggtagaaatg ttcatccagg ttcagctaaa aataaaatgg taaatgctat acatatagct
    22681 gctgaaatat cagaaatgtt cccagcagat gaaagaccag aaacaacaga aggatatgaa
    22741 ggattttggc atttaaattc tataggtgga aatgtcgaga atgtaagtat ggcttatatt
    22801 attagagacc attgtaaaga aaaatttgaa aatagaaaat ctattatgat agagaatata
    22861 gaaaaaataa ataagaaata cgataataga gtggaattgg atttaaaaga ctcttattac
    22921 aatatgaaag aaaagattga gccagttatg tttatagttg atatagctaa agaagctatg
    22981 gaggaattag gtataaaacc acgtttagtt cctgtaagag gtggtactga tggagcaaga
    23041 ttatctttta atggattacc ttgtccaaat atatttactg gtggattaaa cttccatggg
    23101 aaaaatgagt gtataccagt tagttctatg gagaaagcta ctaaactaat tgtaagaata
    23161 gctgaaaaat atgctgaaag agtttagtta aaaagtattt aaaaattaag catttaaagt
    23221 tataatagct atatcattct ttataagtca agctgattat aagcttgtgt tgtacttaaa
    23281 atggtatagc tatttttgta attattttta taaaagataa ttatttttag tttagggtat
    23341 caataaaaag tatgaatatc aataggtatt aacatttaaa agggggaatt gtattgaaaa
    23401 ttaaatctaa aaaaataata gcatttgcac tttcacttgg gattatgttg ccaagttgtg
    23461 tatatgcaga caatattagt aaaaatcaaa atgcacaggt agatatgaac aattatcttc
    23521 aaggtagaaa atctccaaag gaggaaaaac ttattggtga agatagattt gatacagcga
    23581 taaaaatatc tcaaagtggt tggaataatg gttcagaaag ggtgtttcta gttaactcaa
    23641 attcattgcc agatgcattg gcttcaactc ctttggcaag taaattggat gcaccaatac
    23701 tgcttacaaa taaaaatagt ataccatata atgtaacaga ggaaataaaa aggcttaatc
    23761 cagcagaagt tattttaatt ggaagtgaag gagctatttc atcaagtgta aaagaatctc
    23821 ttgaagatat ggaagtatta gtcagtagaa tcggtggagc agatagagaa gaaacatctt
    23881 tacttcttac tagacagcta gatgatactg gtgatttagg tgtctcaaaa gtggcagtgg
    23941 ttaatgggta taatggactg gcagatgcaa cttcaatatc aagcccagct gcaagtgata
    24001 atacagctat aatatataca ggtaaggata gcataagaag tgaggcaaag aattttatta
    24061 cacaaaactc aactaagaca tacatagtag gtggggagta cagtataagt aaaaaacttg
    24121 aaggacagtt agtaaactca gaaagactag cagggacaga tagaaaagat actaatgcga
    24181 aagttttaga aaagttttat ggaaaatcaa gtaaggttaa taatatgtat tttgcaaaag
    24241 atggaagtgg tagagaagct gatttagttg atgggcttgc tgctggagta ttagcatcaa
    24301 aaacaaagtc acctgtagta cttgcaagtg gaagtttaag tagtgcacag aaaagtttta
    24361 taaaaaaagt aaaggctgat aaatttgtac aagttggtgg aggtaaaaat agtaaaccat
    24421 atgcagaagc attagcctta cagtaatatt gtgtaaatca aaattaatat ataataatag
    24481 caagtaaacc aagttgcact ttaatatata taataaaata agtaaactaa gt
//
LOCUS       DAMJWS010000066        24274 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMJWS010000066 DAMJWS010000000
VERSION     DAMJWS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.066"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            66..887
                     /locus_tag="PC399_002625"
     CDS             66..887
                     /locus_tag="PC399_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2593478.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            908..2104
                     /locus_tag="PC399_002626"
     CDS             908..2104
                     /locus_tag="PC399_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2593479.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            2271..5669
                     /locus_tag="PC399_002627"
     CDS             2271..5669
                     /locus_tag="PC399_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2593480.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5679..7352
                     /locus_tag="PC399_002628"
     CDS             5679..7352
                     /locus_tag="PC399_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2593481.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7625..7819
                     /locus_tag="PC399_002629"
     CDS             7625..7819
                     /locus_tag="PC399_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2593482.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    7988..8221
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8298..9695
                     /gene="asnS"
                     /locus_tag="PC399_002630"
     CDS             8298..9695
                     /gene="asnS"
                     /locus_tag="PC399_002630"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2593483.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9868..11043)
                     /locus_tag="PC399_002631"
     CDS             complement(9868..11043)
                     /locus_tag="PC399_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2593484.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11462..12826
                     /locus_tag="PC399_002632"
     CDS             11462..12826
                     /locus_tag="PC399_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2593485.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12848..13339
                     /locus_tag="PC399_002633"
     CDS             12848..13339
                     /locus_tag="PC399_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2593486.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13611..14282
                     /locus_tag="PC399_002634"
     CDS             13611..14282
                     /locus_tag="PC399_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2593487.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMKRLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14367..15233
                     /locus_tag="PC399_002635"
     CDS             14367..15233
                     /locus_tag="PC399_002635"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2593488.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15266..16768
                     /locus_tag="PC399_002636"
     CDS             15266..16768
                     /locus_tag="PC399_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2593489.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16898..17350
                     /locus_tag="PC399_002637"
     CDS             16898..17350
                     /locus_tag="PC399_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2593490.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17510..18313
                     /locus_tag="PC399_002638"
     CDS             17510..18313
                     /locus_tag="PC399_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593491.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18371..19258
                     /locus_tag="PC399_002639"
     CDS             18371..19258
                     /locus_tag="PC399_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2593492.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKDMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19277..20125
                     /locus_tag="PC399_002640"
     CDS             19277..20125
                     /locus_tag="PC399_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2593493.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20188..20877
                     /locus_tag="PC399_002641"
     CDS             20188..20877
                     /locus_tag="PC399_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2593494.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(21207..22397)
                     /locus_tag="PC399_002642"
     CDS             complement(21207..22397)
                     /locus_tag="PC399_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2593495.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYAWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLDDGIDTAGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSERGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEPKVILTPEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLRDSEIGYYIDTPKTKNGKRKIPLTE
                     RAYQALQRILKNRVKAQPLVVGGYSNFLFLNREGLPKVAGNYVGMVRGLIKKYNKYHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(22475..22678)
                     /locus_tag="PC399_002643"
     CDS             complement(22475..22678)
                     /locus_tag="PC399_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2593496.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(23060..23308)
                     /locus_tag="PC399_002644"
     CDS             complement(23060..23308)
                     /locus_tag="PC399_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2593497.1"
                     /translation="MKHTFKKPSYYLLSQAMDGDEKAIEKVLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            complement(23312..23731)
                     /locus_tag="PC399_002645"
     CDS             complement(23312..23731)
                     /locus_tag="PC399_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2593498.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQQGLKRRKEKEISFC
                     ELPEIMVENLAVWDDYETDYTIFNVCGNDIRVYDDELAEALKQLSERNRETLLMYYFL
                     EMNNEEIAKKQNISRSGVFQNRHNSLALMRKLLKEKR"
BASE COUNT         9111 a         2913 c         4367 g         7883 t
ORIGIN      
        1 tatatcaaaa atagtaataa agtctatata catttagaaa atactgttta aaaggagtag
       61 gatttatgat aaaaaaagag atatctaact ttagaggaag tagtgattat gtagtatcac
      121 cagaattgat ggcatcagtt aatgttgcta ttgctcttga aaagccactt ttgattaagg
      181 gcgaacctgg aacaggtaaa actatgctag cacaagcaat ttcaaatgaa ttaaaaaaag
      241 atttagtaat ttggaatata aaatcaacta caaaagcaca agaagggctt tatgtatatg
      301 atactgttca aagactttat gatagccaat ttggaggaga aggtgtagat gatattagca
      361 agtacataaa atatggtaaa ctaggagagg cctttagctc aaatcaacaa gtaattcttt
      421 taatagatga aatagataag gcggatttag aatttccaaa tgacttgctt tgggaattgg
      481 ataaaatgga gttttacata aatgagacaa aagaaactgt aagagctaaa caaagaccaa
      541 tagttattat aacttcaaat gcggaaaaag aacttccaga tgctttttta agaagatgca
      601 tatttcatta tatagaattt cctgatagag acatgatgga agaaatagtt aaagtacatt
      661 ttgataaagt agaagaacac ctattggaac aagtaatgac tactttttat tggattagaa
      721 gtttaaagga catacaaaaa aagcctagta catcagagtt aatagactgg atacaagcat
      781 taactttgag tggaatgcct attgaaaaaa tagaaaaaga agttcctttt gcaggaatac
      841 tacttaagaa taatgaggat attgaatcta tgcaaagaca cttatagtta ataggagctg
      901 gataattatg tttattgaat ttttttattt attgaggtca agaggattaa atgtatcttt
      961 aaatgaatgg atgactttaa ttgaagcatt ggataaagga ctgtgctatt caaacttttc
     1021 aaatttttac tatctttgca ggatgatttt aataaaaagt gaaagtgatt ttgataagtt
     1081 tgatgcagtg tttttggagt attttaaagg tatagaacac caagaagaaa ttccagaaga
     1141 gattatgaat tggcttaaaa aaccagatat agatttagaa gagtttgaaa gattagaaca
     1201 aaatccaaat ataaatcttg ataaattaag agctaaattg gaggaacgaa taaaagaaca
     1261 agattctcct cataatggtg gtaattattg gataggcaca ggaggcactt cagagttagg
     1321 acattcagga aaagggcaaa ctggtatacg tataggtgga aattctactt atggtagagc
     1381 tgttcttgaa gttgcaggag aaagaaaata tagagatttt agagatgatg aagttttaaa
     1441 tatgcgtcaa tttcaagttg cactaaagag acttcgtcaa ttttctacta gaatagatgc
     1501 tccaaagaca gagcttgatt tggataagac tattgaagag acttgtaata atgcaggata
     1561 tttaaagtta ttttttgaga agccaagaaa aaatacagtg aagcttttat tgcttatgga
     1621 ttcaggtggt tcaatgagag ggtatagcac tttatgtaat acattatttc agtctgtaag
     1681 taaatcaaat cattttaaag atgtaaagat atattatttc cataactgta tttatgatag
     1741 attatttaca acacctgaat gttggttaag taaatctatt aatactgaat ggatattaaa
     1801 aaatatagat aaaaactata aagttataat tgttggagat gcttctatgt cgccttctga
     1861 gttgcttcat attggtggta actatcgtgg tccatataat tacacacctg gtatagagtg
     1921 gcttaaaagg tttaagagaa agtacagtaa aattgtatgg atgaatccag aacttagaga
     1981 tggatgggat tcaattaatt attggtatca aactcaaaga atgatacaaa gtgaatttaa
     2041 tatgtttcca ttgactgtaa aaggtcttga gaaagcattg aaaaatttaa tggtttcaag
     2101 ataaagtaaa tataaatgat ggtatgattt attcaaaatt gtaccatcat tatttatttt
     2161 tttaatatac ctattataaa ttgtataact gaaacttaaa aaattatata gtctaataca
     2221 ttttagactc acacaaaaca tatacattaa taataatttt agaagtcgag gtgattataa
     2281 taaattatga attaattgta aaatacaatg gagatatatt gaggttagaa gaagaattag
     2341 gtgtatcagt agagatactt aattcatcat atgcaataat aacatcaagt aatgaagaag
     2401 atgtaaatat acttttaact tatccagaaa ttgaatttat agaaaagcct tttatattac
     2461 agactcagga tgtgcaaagt ttttcaagta caggaataac aggatttaaa aatagaactg
     2521 gtttaactgg aaaaggaact ataattggta taattgattc gggaatagat tatactctac
     2581 ctgtgtttag agatagtgat ggaaggtcta agatattata ttattgggac caatctattc
     2641 aaggaaatcc accagaaggt tttagagaag gaacattata tactaatgaa gatataaata
     2701 atgcaataga tggaagtatg tatataccta tttcaacaac tagcttacat gggacacatg
     2761 ttgcaggtat ttgtgcaact attgcaagtg atgccagaat aatagtggtt agagttggaa
     2821 atatacaaac cgatattttt tcgagaagta cagagtttat gagggcaatt aaatttattt
     2881 tagatagggc attagaatta agaatgcctg ttacattaaa tataagttat ggaagtaatg
     2941 aaggttcaca tagaggaaca tctttatttg aacaatatat agatgatatg tgtttatttt
     3001 ggaaaaacaa tatagttgta gcagctggaa ataatgcaga taaaggaggt cataaaagga
     3061 ttaggcttca aaataatatt acagaagaag ttgagtttat agttggggaa ggagagcgta
     3121 tattaaacat aaatatatgg ccagattttg ttgatgattt tagtgtacat ttggtaaacc
     3181 catcaaataa tcaaacacaa gcaatctctc taacttcagg tgagataagg aatactttgg
     3241 gtgaaactag aataactggt tatttttacc ctatagcacc gtattctctc acaagaagag
     3301 ttactctaca attaagttca aatacacaga taactcctgg gttatggaaa attgtatttg
     3361 aaccaataga tatagtaact ggtaatgtaa atatatattt gccaacttct gaaggtctaa
     3421 atagaaatac tagatttctg attcctactc aagagcttac tgtaacagtt cctggaacag
     3481 caagtagagt tataacagta ggtagtttta attcaagaac agatatagta tctatatttt
     3541 ctggtgaagg agatacacaa ttaggtgttt ttaaacctga tttattagct ccaggtgaag
     3601 atatagtatc ttttttacca ggaggtacaa gtggagcatt aactggaact agcatggcta
     3661 cacctcatgt tacaggggtt tgttcattgt ttatggaatg gggaattgta aatggaaatg
     3721 atttattttt gtattcacag aaattaaggg cattacttct aaagggagca aggagattaa
     3781 gtaatcaatc ttatccaaat aattcatcag gatttggttt tttaaattta tcagatatag
     3841 atttgtacac tctatctaat ataaatcaag atttagaaac agaggatatt ggatatagaa
     3901 gtattaataa gtcatttaaa gatgaggaaa atagttataa gtttatagat ggttataata
     3961 tgcaaataca taatgactta gagaatgaaa tttatataag taaaaatgca agtagacaat
     4021 caggaatact atcaggaata gatatagtcc atactcctga atttgaagaa gaactagctg
     4081 ggttaggtat gagtcaaagg ttttttaaga taagtgattc attaggtgta ctatctataa
     4141 ataatacaga ctataatagc atacaacgag ttttgcaact tccttcaatc ataagaactg
     4201 tatcgactac aaagatgaca ttacttggag aaattaatcg aggtactttt ggaggtgtag
     4261 ttgcaacaga agaaatgggc gtaaatttct ttaaaaataa tcctaatatt aatataacag
     4321 gaagagggac tctaatatca attgcagata caggaataga ctatctacac ccagatttta
     4381 tttatccaga tggcacttca aaaatagtat atttatggga ccaaacaaaa gaaggaactc
     4441 ctcctgatgg tttttatatt gggacagaat atacacgaga agatataaat cgagctatcg
     4501 cagaaaatga ccctagtcta tctcaggatg aagttggtca aggaactatg ttgagtggga
     4561 tatgtgcagg gcttggaaat gtaaatagtg aatatgcagg tatagcagaa gattctgagc
     4621 taataataat aaaactcggc aagatagatg atttttataa tagtgcaatg ctgtttgctg
     4681 catctcagta tgcatataaa aaagcttttg aacttggaag accattagtt ataaatatgt
     4741 ctttaggtac cagtagccta gcaggtttaa ccaataggag taatagtgaa aaagcgttct
     4801 ttactagagg gttatgtata acggcaggag caggaaatga gggcaataca cagacacata
     4861 catctggaat aataccatat gttggaggtt ctgttgaagt tgaattagag ttaaatgaag
     4921 atgaggaaga attatcatta gaactttggt taaatagacc agataaagct gatgtaataa
     4981 tagtatctcc cactggtgaa gaaagtaaaa gtgttggaat ttcaaattat aataaagtaa
     5041 caggtttatt tgatttagag gggactgaat attctataac atatatatac ccaacaactt
     5101 tttctggaca gcaatttact aatgttactt taaagaatgc aaaaagaggt gtatggaaga
     5161 ttcgattagt aggagtttat attataacag gaagatataa cttatacctt ccaaatagag
     5221 aacttttaaa aagtggaact agatttagag aggtagaccc tttttacact ataaattatc
     5281 ctgcgattca agatgattta ataaccatag gagcctacaa tactattaat ggtagtttat
     5341 ggcaaagttc atcaagaggg ccaactattg aagacaggtt aaaaccagat atagtagcac
     5401 caggtgttaa tataatagct gcttatcctg ggaacacgta tgcaactata actggtacag
     5461 cagcagcaag tgcacatgca gctggtgctg ctgcaatgta ttttcaatat acttttgttg
     5521 acggtagata tccaaaccaa gcatatgtgc aaaaaataaa aacttttatg caagctggtg
     5581 ctagaaaaga ttcaaacaca gtatatccaa atacaaattc tggatatggg ctcttggatg
     5641 ttagaggaat gtttgatgtt ttaagatagg agggatttat ggaaaaatct tattgtataa
     5701 tttatcaagg tgatatagaa tctgccctac aagaaaatgg aataaatagg tatatggttt
     5761 taaatagtca gcttgcagta atatatgtac ctttagattt tgatgaaact attttaaata
     5821 atataataca agttgcttgg tgggaagaat ctgaaccaat gagcagtcta attgaaataa
     5881 ctaataatgt aaataatgga gaaaccatta caacagcagc tgaaactgat tatatttatg
     5941 aaaatccata taatgatata acagggagag gaattttatt agcagttata gattctggga
     6001 tagattattt gcatcctgat tttataaatg atgatggaac tagtaaagtt ttatatttat
     6061 gggaccaaga agcaaataca aatccacctc cagaaggttt tatttttgga agtgaattta
     6121 caagaagtga gttaaatata gccataaata gaaatgatgg gagtttgagt caagataata
     6181 taggtactgg aactttagtt tctggtatat tagttggaaa tggaaggata aattctcagt
     6241 acagaggtat tactacagaa tcagacttaa tagtagttaa attaaaatca tatacagata
     6301 cttattatgc tggaaggata aattacagtg tttcagattt tttagcagct ataacgtatg
     6361 ttactaacat agcaagaaca gaaaataaac cactgataat aaatttgact attggagtta
     6421 agtcaagtgc tgtagcaact acttcaatac tagatacttt taatatattg agtagtgctg
     6481 gtgtggtagt agtgagtggt gcaggaaatc aagggaatac agatattcat tattctggca
     6541 gatttagcag tgtgggagaa gttcaagatg taataataca agatggagat gactatgctc
     6601 ttgatataac actaaatacg aatggtccag ataaagtggg agcacagata atatctcctt
     6661 caggtgaagt tagtcatgat ataaggtatt ctccagactt ttatatatat agaggaaaat
     6721 ttaacttaga aaatactact tatgcaatga gatttattta tccatatatt acctctggaa
     6781 aagaaaattt ggagataaga ttaagagata taaaacctgg agtatggatt ttaagattga
     6841 cttcagaact tataataaat ggagaatatg acatatactt gccaaataaa aatttgatag
     6901 ctccagacac aaggttttta gaccctgatt cagtagctac tataactatg tatgctgcta
     6961 gtgatgatgt aattacagtt ggtacattta acaataagac tgatagtatg tggattggtt
     7021 cttcgaaagg accaattaga ggaagaggaa ttaagccaga tatagtagct tcaggtgtag
     7081 atataatatc cacatataaa aatggaacat ataatacagg aacaggaaca ggagtgagca
     7141 gttctatagt cacaggtgtt ttagccttat tgatggaata cttagaaaaa caggataatg
     7201 taccgagatt atcattgttt acacaagttt taaaaacata tttaatattg ggtgctacaa
     7261 aacttgagat atatacatat ccaaatgtat cgcagggata tgggatttta aatttaaaaa
     7321 atacgattca acagatagca aatactctat agataagaac ctatgtaaaa ttataattga
     7381 ttattagttt aattgtatca aatactttag atagagtaga aaaatagcat aaaaaataaa
     7441 tatgaaaatt aaaaaacagt tttatatttt gaatacacaa agtaaatatt ttgtatataa
     7501 ataggtataa attaagtatt gaaaagatat gatgttaatt taaaaataat tataatttag
     7561 acttatgaat tttatttgta aaattgctaa aactattaca aaataaataa tagaggtgat
     7621 agttttggaa agtaaaaagg caagagcatt aaaaggtaaa gataataaaa gacttaaaag
     7681 ttcactttta gttggaaatg agtcgacagc agcatgggct gatgttgaaa aattaaaacc
     7741 tcatagtaaa gtttcaatac cttcaattaa gggtgtcgaa gaagcaaaag actgggttga
     7801 caatggaagt aaattataaa attttggaaa ttgaagtaat aaagaagtaa tgaagataag
     7861 tatttattat cattatgtaa ggtagaatta ttatatttta aataacaatc agtatcaatt
     7921 atataaattt attgactaaa aatttataaa ttggtataat tttgttaatt agcagaaata
     7981 ataaaagata aatactgtga agagaagagt agatataaaa tgtagttcta gcgagttgag
     8041 gatggtgaaa gctcaatacg aggtttatat tgaagaacat cttggagttt ctaatcgaaa
     8101 gcttatttag taagtagact tagacggaag gcaccgttat aggctttaag cattgcttaa
     8161 ctaagaggtc attatgacaa ctagggtggt accgcgagag taatagtctt tcgtcccttt
     8221 atatagggat gaagggcttt ttttattgaa attatattta aaagaaatta ataacaatta
     8281 tactaggagg atttaaaatg caaaaggaat ttataacagt aaaagaattg tacagagaaa
     8341 aagaacagta catagggaag actgtaaaag tagctggatg gataagaaca tctagagtat
     8401 ctaaaaattt tggatttata gagctaaatg atggaagctt ctttaaaaat atgcagatag
     8461 tatttgatga gaaagtagaa aattttaaag aggtaggaaa attagcaata agctcatcta
     8521 tacttgtaga gggagagtta gtatctacag aaggggctaa acaaccagtc gaaatacatg
     8581 caaaaagcat agttgtagaa ggggaatcag atagttcata tccactacaa aagaagagac
     8641 atacgcttga atatttaaga acgatagcac atttaagacc aagaagtaat actttttcag
     8701 ctgtatttag agtaagatca atagctgcct atgcaataca caaattcttc caagaaagaa
     8761 actttgtata tgcacattct ccaatcataa caggtagtga ctgtgaaggt gcaggagaga
     8821 tgtttagaat aacaactatg gatttaaatg atattccaaa aactgaagat ggcaaaatag
     8881 actatactca agatttcttt ggaaaagagg ctaatttaac agttagtggt caattaaatg
     8941 ccgagataat ggcactttct tttagaaacg tttatacttt tggacctaca tttagagctg
     9001 aaaactctta tacaggaaga catgcttcag aattttggat gatagagcca gaaatagttt
     9061 ttgcagacct tgaagataac atggaacttg cagaagatat gataaaatat gttataagct
     9121 atgtattaga aaatgctcct gaagaaatgg aattttttaa tagttttata gataaaggat
     9181 tgttagaaag gttaaataag atagtaaact ctgagtttat tagaataacg tatactaaag
     9241 cagtggaatt attaattgaa tctgggcatg aatttgagta tcctgttgag tggggatgtg
     9301 accttcaaac tgagcatgaa agatatataa cggagcaaat atataattgt ccagtgtttg
     9361 ttacagatta tccaaaagat ataaaagctt tctatatgag aatgaatgaa gatggtaaaa
     9421 ctgttagagc aatggattta ctagttcctg gagttggaga aatcgtagga ggttctcaaa
     9481 gagaagaaag agaagatgta ttgttagata gaattaatga aatgggatta agagaagaag
     9541 attattggtg gtatttagaa cttagaaagt ttggtactgc tactcactct ggatttggtc
     9601 ttggatttga aagaataatt atgtatatga caggtatgtc taacataaga gacgttatac
     9661 ctttcccaag aactcctaaa aatgctgaat tttaataatt atatataata aaacttatat
     9721 aactttaagt ataaacacta aaatgacttg catatttatc tattttagag atatgcaagt
     9781 tatttttatt gtactataaa tatataattt gattactttt attttgcgat ataatactta
     9841 taggtttatg taatagtatg taacctttta ataaaatgca acacaattac gtccctgttc
     9901 ttttgcagta tatagagcct tatcacaatt acttataaac tcatttaatg ttattgtctt
     9961 tccaattttc tttgtataaa ctccaatact aacagttaca cattttaaag gaacatcatc
    10021 acgctcaaaa ttgcaggact ctataatttt tcgtaaacat tctgatttaa gtttagcatc
    10081 tgcctcatta cagttaaaaa tacatataaa aaattcctca ccaccatatc gtatagcatg
    10141 ttcattttca gaacttgtaa aatctaaaat tactttggca atattttgta gacaatagtc
    10201 tcccttttga tgtccataat tatcattaaa ttgtttaaaa taatctatat ctatcatcat
    10261 tccagttata ggttcttgat ttgcatggct gattaatttt ttttgtagaa tatcttcaag
    10321 gtatctacgg ttgttcattt tagtaaggtc atctgtcata actttgtcgt taagtaaatt
    10381 atttatatga gatatttctt catattgttg actaattaaa agtttatcat agtaagtaaa
    10441 tacacgattt ctatatagca ttgtagaaac aaaaatggca agggatgtta taaaaaatgt
    10501 gttgacaata ttactaaact cattgcctgg tatagtttga atcccaggta agaataaatt
    10561 aagaaacaaa aagcatcctc caaaaactat tactgattct ccaaatttta atactgagaa
    10621 agctgcagct gctaacataa tataactaaa tacacttagg tcatttccac ctaactggtc
    10681 aagtagagta attacacaac cccataggca gaaaaaagtc acataaatta tccataaatt
    10741 tatataaaac gtacagcaat cctttttttt attccaaagg taagaaaata caaacataaa
    10801 gacagaagat acacaaaata gtgttacata catataaaaa taccattggc gtcttggtgt
    10861 gacaaaaggg cctccttctc tagctgagat tgatatcatc ataattattt gagatattac
    10921 tattattatg caaatgattt tacccgtatg ataaagataa ttgcagagtt ctttctcaac
    10981 attgtctttg tgttttttgt ctagaggaaa tccaaaaaat ttttttattt tttctaagtt
    11041 catataagcc tccattaaaa ataaagcata aaaataagta ttcctttcta tcattagata
    11101 tttggaatac ttcgtttatg atttaattat atcaaaatat attgtgtgta acaatctaaa
    11161 attgtgatag ataaaattta ttagataatg tcgagtaact ttattgatta atactttatt
    11221 ataattaaga tttacagata gtttactact gaaaataaag aatagaataa atttaaatta
    11281 agcataataa aatataaata ataaagttcg cattaacaaa ctatttaaat atcaagacct
    11341 gttaaaaaac aacagcaata aacaaataaa atagataaat atatccaatt aaggtataat
    11401 taagttttat atgatatagt tatatttgat aatttacaat agtaaaactt ggagggaaaa
    11461 aatgggtgaa aagaaaataa ataatatgaa ttattcacat aataattatt ccaaaaagac
    11521 aggtgaacca actagaaaaa taaaaaatag ccatatcact cttaaaagta aagaagaaaa
    11581 aatttgttct agttgcaata aagaaaataa tactaaggat aattactgta aattttgtgg
    11641 aaatgaactt tatgaaatag catcattaag accacttgaa acaaaactag acttgaaatc
    11701 taaaataaaa gagctttcat atcatgcaaa taaaagaggt gtatttttaa caacatttac
    11761 aaccatattt atattgttta ttattgcatt gatatttaaa gctattataa ctattcaatt
    11821 taatgatatt agttatttag taaatccagc acatataatc ctagctctca acttgggtca
    11881 aattagtgta tctatgtcta ccatgatggg ttctgggttt ataaatgcca atataggttt
    11941 gttgatatta cttataatcc ctattttggt attattaata tcaaacctaa tttttatgag
    12001 aaaaagatgt agagattcaa aaactgtatt agcaaattct ctaggagttg gaatttttta
    12061 tggattagtt ttagctattt tatctatttt tactaatgta aaaactagtt cacatagtat
    12121 gttagaatat ggatatgcat tagaatatag ttatgaattt tttagtgttc ttttaaatgg
    12181 attcgtatta gggtttatat gtacatacat aactacttat aaaaaaagtt atgaaaaaga
    12241 aaatatgtat ttatctcttt tttcaaattc tataagaaca ttcatattag gttatgtttt
    12301 agtattagta atactattgg tactaactat atctgatagt agttacttaa atgaactaga
    12361 tatgagtagt tattcaaatg gattaaattt atttactata ctcccacaaa tagctagtta
    12421 tatgtgggct tttgcaaatg gaatttcagt aaccataata aactcaacag tatcaatgtt
    12481 tactttaagt tcttcatctt tatttggaga tactaaatta atgttttatg caatgggagc
    12541 attgtctatg ttaatattat tattaaatgg atacaagcta agatttaagt ataatacaga
    12601 tagtataaga cctataatag tatttagtat atattatgca tttttgatgg gaatattggc
    12661 cttatttagt acatttatac tagatagtaa tattaatttc tttaatacaa ctaattatgg
    12721 aacaacttta ataatgcaat ttaaagtctt acaagctatt gtaatttcat ttgtatatag
    12781 ttttgtgata tctttaatag gatataagct aaattcagct gattaaatat gattggaggg
    12841 aatttctgtg gaaggaaata ctaataataa agtagatgat aaattttcta aggttaagaa
    12901 aaagtttggt agtggaaaat ttaaagttga attatataaa gaaaaatcaa aattaaatag
    12961 agagattgat gatattcaag aaaagaaagc aaagatattc ttggaaatgg gaatactgac
    13021 atatcaaaaa attagagaag attctattaa tgataatagt tttgataaat tctgtgatga
    13081 gttactagaa cttgataagt taatttatga aaaaaatatg gaaattaatg aaatggaaat
    13141 ggcagaaagt aatgtaactt gtgagtgtgg ttatgttgga aatgtaaatg ataaatattg
    13201 tgctgaatgt ggtaaaaaat ttgaattgga aaatgaatgt gatgatttta taatttgtgg
    13261 ttattgtgaa agtgagatag atacagaggc tgaattttgt ccatgctgtg gaagaaaaat
    13321 tataatggat ttagaataat attgggaaac ctatgtatag tatacatggg tttttttatt
    13381 gaaattattt aaatggactt ataacagtag ttatataaaa tgaaaataaa caccaaaaaa
    13441 atgaaataaa atttctttta aatgtaaaaa acaagctaat atataaaata aatcagtaaa
    13501 aatatagatg cgagaaaaaa tatgaaaaaa tattgcaaag aaaaagtttt catgttatta
    13561 ttaatacata aatcaagaaa ataaatttca gaaacgaggt aatttaaaaa atgctagata
    13621 aggtaaaggg aagattaata gtatcatgcc aagctttaga aaatgaacct cttcatagtc
    13681 cttttataat ggggagaatg gctaaggctg ctatggaagg tggagctgtt ggaattagag
    13741 ctcaaggagt tgaagatatt attgaaataa aaaaagttac tggacttcct gttataggta
    13801 ttataaagag aaactatgaa gattcagata tatatataac gccaactaaa aaagaagttg
    13861 atgaactttt aacaacaggg tgtgaaatga tagcattaga tgctaccaat agagttagac
    13921 caaataatga agacttaaaa gaacttataa agtatataaa agaaaacggt gttctagtaa
    13981 tggcagatat ttcaaattat gatgaggcta tcaaagcaca agagtacggt gtagattgcg
    14041 tttcaactac actatctgga tatactccat atactaaaac attagaaggt ccagattttg
    14101 tattaatgaa aagattagtt aaagatttag aaatacctgt aatagctgaa ggaaaggtaa
    14161 atactcctca agatttaaaa aaggtctttg agttgggtgt tcattcatca gtagtaggtt
    14221 cagcaataac aagaccacaa ttaataacag aaaaatttgt taaagcaatt gaaattaatt
    14281 aaaaattaat ttataaatta aaaaattatt atatttaact atatttgaaa attaaaattg
    14341 gaggtaaaga tatgagaaca acagatatga agggaattta ttcagcatta ttagtttcat
    14401 ttgataagga aggaaatata aatgaaaaag ggttaagaca aataattaga cacaatatag
    14461 atgtttgtaa agtagatggt ctttatgtag gaggaagtac aggggaaaac ttcatgcttt
    14521 caactgatga aaagaaaaga atatttgaaa tagctaaaga tgaagtgaaa gaagaaataa
    14581 aattaatagc tcaagttggt tcagttaatt taaaagaagc tgtagaactt gctaaattta
    14641 caactgattt aggatatgat gcaataagtg ctgttacacc tttttactat aaatttgatt
    14701 ttgaagaaat aaaacactat tataatacaa taataaattc tgtagataat agattaataa
    14761 tatactctat accattttta actggtgttg atatgagttt agaccagttt ggagaattat
    14821 ttgaaaatga aaaaataata ggtgttaagt ttacagcagc agatttctac ttattagaga
    14881 gaatgagaaa aacattccca aataaattaa tatttgcagg atttgatgaa atgatgttac
    14941 cagctacagt tttaggtgta gatggggcaa ttggttctac attcaatgta aacggtgtca
    15001 gagctagaca aatattcgaa ttaactaaga atgaaaaaat atcagaagca cttgaagtac
    15061 aacatgtgac taatgattta ataacagata tattaggaaa tggcttatat caaacaatta
    15121 aattactact tgaagaacaa ggtgttgagg ctggatactg tagacaacct atgaaagaag
    15181 caactgatga aatgaaatca agagcaaaag aaatatacag aaagtatttt taataaaata
    15241 cttttagtat gggaggatta gtcaaatggt aggatttact tggatagatt ttgcaatact
    15301 agtggtttac ttattggcag ttttatttgc aggattgtta ttttcaaaaa aggaaatgaa
    15361 gggaaaagag ttcttcaaag gtgatggaac tattccgtgg tgggttacat cagtatcaat
    15421 atttgccaca ttattaagtc caatatcatt tttatcatta gcaggaaatt cttatggtgg
    15481 tacttggatt ttgtggtttg cacaattagg tatgtttata gcaatacctt tgacaataaa
    15541 atttttctta cctctatata gtaggttaga aatagataca gcatatgaat acttagaaat
    15601 gagatacgaa agtaaaggtc ttagagtact tggagctttg atgtttataa tatatcaaat
    15661 aggacgtatg tctattataa tgtatcttcc atcaatagtt ctggctggtc ttacaggaat
    15721 acctgtaaat gtactgataa tagttatggg tgtaatagca ataatttact catatacagg
    15781 tggattaaag gctgtcttgt ggaccgattt tatacaagga atggtattaa tagtaggtgt
    15841 tacagggaca ttgttctatt taatagcaag tataaatggt ggttttggaa cagtaatgga
    15901 tactcttaca actggacaca agttcttagc aagtaatgaa gttatgtttg ataaaaatat
    15961 actatctaca agtgcattta taatatttgt tggagctggt cttaatactt tttcatcata
    16021 tgtatcgagt caagatatag ttcaaaggtt tactacaaca actgatataa agcaacttaa
    16081 taaaatgaca tatggaaatg gtgttttatc aatgggactt gcaacagtat tttatctaat
    16141 tggaacttgt ttatttatat actatactca aaacccagat ttagctcaaa cagttcaaca
    16201 agaccaagta tttgcatcat atatagctta tgaattacca gtgggtataa caggaatact
    16261 acttgcagcg atatatgcag cttctcaatc aactttatcg acagggctta attcagttgc
    16321 tacaagttgg actttagata tacaaactat cataacaaaa gatataagta tggaaaagca
    16381 aactaagata gcacaatata tatcattagg tgtaggtata ctttctatag ctgttgcaat
    16441 agtattagca aatggagaaa taaagtcagc atatgaatgg ttcaatagtt ttatggggct
    16501 agtactaggt gttttagcag gaatatttgt acttggtgca ttttgtaaaa aagcaactaa
    16561 aatgggagca tatgttggat tcatagtatc agcaatttta gttgtttatt taaaataccg
    16621 tatgccagaa gtaacttctt gggcatattc tctaataaca atcacgactt ctgtagtggt
    16681 aggtacaata gttagtgcaa tagaagctaa agttaaagca aaagtatctt taccaaaagc
    16741 tgagactact gtttattatg aaaaataata tttaaatgat aaattgtctt atagagtgat
    16801 tagataaata atttagttac tctataagat tgtataatat actttgtata caaaagtata
    16861 aaaatagact ttataattat atagggagat agtgcttatg atatttggaa atataaatca
    16921 tagtaagaca tattcaacat tgcatgaaga tttacttaga tgttttgaat atgctaaaga
    16981 taattcatta atagattatg aaaaaggtac ttatacaata gatggtgatg atatttttgt
    17041 gaatattgta gagtataaaa cttgtgaaaa agaagataga ttctgggaag ctcataagaa
    17101 gtatatagat gttcatttga tgcttgatgg atgtgaacga atagatataa attttataga
    17161 aaatttagaa caaaaaatat atgaagaaaa gggcgatttc ttgcctcttg atggtaaaaa
    17221 taatggatac atggaactta gaaaaggtga ttttttagtt tgttatccgg aagatgctca
    17281 tatgacagga ataaaagttt tagaaaaaga aaatatcaaa aaagctattt ttaaggtttt
    17341 ggttagataa tgataattta tataaccact agggctgaaa atagtgattt aactttcagc
    17401 gattagtgag acttcgtaaa ttttagttag attactattt tcagccttag ttttttatga
    17461 gaaaattaaa aataattaaa agcttaaaat tttgtaaagg agttaactta tggaaaatat
    17521 agatgtattc ttattaatag gacaaagcaa tgctagagga ttgggaaatc caaaagagag
    17581 catcattcca aatgaaaatt gttttgaata tttgagtact gatgaaatta taaatatgag
    17641 atgtgagcta gagacttcag aaggtgatgg tactatagca cctgcatttt caaatgaatg
    17701 gaataagctt actggaaata aagtttgttt tattcacaat gcaaaagatg gttcaagaat
    17761 aaaaaactgg aatcatgata ataactggtt tctaaatgac actatagaaa aatttaatac
    17821 aggctgtact accttaagta aacattataa tatagaaaat aaatacgtta tttggattca
    17881 aggtgaaagt gatgcaaaat atggtagtga tgtattgtac tataaagaaa gtctaaaaaa
    17941 aattgcgtat aggcttaaag aagaatgtat gattgataaa atgtttgtaa gtttaactgg
    18001 ttattggctt ggagaagatg aatattttat tagaacaaga cgaattgcag cagcacagga
    18061 gtcggcttgt agtgaatgtg atatattatg tgtaggaagc aaaattgcta tgcagtttca
    18121 tgacagagga ttaactattg atgatgttca ttacactcaa gaaggtctta atatgttagg
    18181 agaggactta tgtaaaaata tttataagta tcatataacc aaagaaaaaa gtgtattaag
    18241 tgatactata gacttatcag aagcaaggaa atatatctgt gaattggaaa agataagtaa
    18301 aagatttgtg taatataaaa ttttaaatag agttgttaat ttataaaaat aatttgtatg
    18361 gagatatgtt atgaaaaatt acgtttgtat agatgttgga ggtacttcaa taaaatatgg
    18421 ttttattaga gaagatggat ttataattga taaatcaagt cttgatactg aagcaaagga
    18481 aaaaggtgga gaaggaatat tagccaaaat aaaagatata gtaaaaaaat atatagagga
    18541 aaatgaaata agtggtatat gtatatcaac tgcaggtatg gttgacccag ttgaaggtaa
    18601 aatactattt gcattagaag aattaattcc aaactataag gacatgcaat taaaaaaaga
    18661 agtagaaaaa gagtttaata taaaatgtga agttgaaaat gatgttaatt gtgctggact
    18721 tggtgaaatg tggcttggag caggtagagg tgcaacatct tctatatgtt taacaatagg
    18781 tactggaatt ggtggatgta ttataataaa taataaactg ataaatgggt ttagtaatag
    18841 tgctggtgaa gtaggatata tgaatgtaaa tggaagttct tttcaagaat tggcatctac
    18901 tagcagtcta attaaaaaag tagcaaaaat aaaaaatttg aatgaaaatg acttaaatgg
    18961 aaaaataatt tttgatttag caaagaataa tgatgaagat tgtttaaaag aactcgataa
    19021 tatgataaaa tcattagcag ttggaatcgc aaatatatgt tatgtaataa atccagaagt
    19081 ggtaatttta ggtggtggga ttatggcaca agaaaagttc ctaaaaccaa aaatagacaa
    19141 agcattaaag gaggttctta tagagagagt ttacaaaaac actaatatag aatttgcaaa
    19201 gagacaaaat gatgcaggaa tgcttggagc attatataat tttttaaaca aaatataaag
    19261 aatagaaggg tttttaatga gtattttaga acaattggaa tcaccaactt ttaaagtcac
    19321 aaaatcagat aaattattaa tagcttatat aaaagaaaat atagatgatg tattttataa
    19381 accaatatca caaatagcta aagagagcaa tattggtgaa gcaactataa ctaggtttgt
    19441 aaaaaaaatg aattttaatg gattacagga ttttaaagtt actttagcac aagaaatatc
    19501 aactatcaat aaaaaaaata ttataaataa aaatatacaa aatgatgagc cagctttgga
    19561 tacagcaaaa aaacttttaa gttcaaatgt aaccacttta gagaacacag tagagataat
    19621 caatagtaag gatgtacatg actgcgcaag gcttataata aatgcaaaga aagtttactt
    19681 cataggtatt ggatattctg gtataatagc acaggattca aactataaat ttatgagaat
    19741 aggtcttaat tgtgtaagtt ttgacagtag tcatacaatg ataatgatgt cttccataat
    19801 ggaagaagga gatttaataa ttgcaatttc acattctggt gaaacagagg agattataaa
    19861 aactgtaaag cttgcaagag ccaataatgc taaaattata tcaattactg aaaataaaaa
    19921 ttctgaatta aaggatattt cagatgtaca cttatcttat gtatcgggag aaacagtact
    19981 ggaaaccggt tcaatatctt ctaaattagc acaatttttc attattgatt tagtatatac
    20041 acaggtagta aaagaacttt ctaatgaagc tattgaaagg aaaattaaaa ctacaaatgc
    20101 tataaaattg tttaaaaatg aataaaataa gaaatttgta atgttttata gagctctctt
    20161 aaaaatttag gaatggagag atattacatg tctaaattaa aagattataa tattgataaa
    20221 gtaataattt ttaataattt aagtactaaa acgaagaatc aacttaaaga aaaagttaaa
    20281 ctaaaaagat tacaaaaaaa agaaatttta ttttatgaaa aagatagctt agacagtgtt
    20341 tatatacttt tagaaggaaa ggtatctatt tttaagataa gtgaaaatgg agagagaaaa
    20401 gtaattttca tattaaatag tggggaagtc ataaatgata ttacacttga cgcttctaaa
    20461 aactctactg ttggctgtga agcatttgaa aattcaattg tagcttatta tagtattgaa
    20521 gaatttttag aaattatgca aaatgatttt caacttacaa aggatattat ttcttatatg
    20581 gaaagaagaa taagacgttt atatagacag ttgaaaaatt ctatatctat aaaagtagat
    20641 aaaaaacttg cagcaaaatt atataggtta agtagagaat ttggagtttg ctgtggagaa
    20701 tggactttac taaatgtaaa tattacagtt acatatttag ctgatatgct tggctgtaaa
    20761 agggagactg tttccagaat gataaaggta ttgcaaaaag aagaacttat taaaattgac
    20821 aataaaggtt tttatgttaa agaaactgaa ttatcaaggt attttaaaaa taattaatta
    20881 tgattaagta agagtattat tagaatttta attaaataaa aatattatta agagaatcta
    20941 tttatagatt cttttttatt gtctaattta ttaattttta ttttacccta ttgaattgta
    21001 taaaaaaaca aataagattc ttttttattc aaaaaactta ctaaattttt gattatcttt
    21061 tcataagttg gtaaatgccg atatatcccg tatttgaggg ctttatcggt attttctttt
    21121 tggaagatac cttgcataag ctggcattta ccagcatgaa aatgcaacca aaagtagtaa
    21181 atgagtagta aatatacgtt ccgatattaa caagccagtc tttccagttc tgcttttgca
    21241 gattggaatg taccgtgtgc gtagtagcca agcgtcatgg ttatgttagc gtgtcccatg
    21301 agatattgca gggtgttcgg gttcattccc ttgtttgcca tattcgtaca ataggtatgt
    21361 cggaatgaat gtggtgtaat gtttggtaac ttgtctttgt ggtatttgtt atacttctta
    21421 atcagtcctc gtaccatacc cacatagttt ccagcgactt taggcaatcc ctcacggttt
    21481 aagaatagga aattgctgta accaccaaca accagcggtt gagcttttac tctgttcttt
    21541 agtattcttt ggagtgcttg ataagccctt tccgtcaatg gaatttttcg ttttccgttc
    21601 tttgtttttg gagtgtcaat atagtagccg atttcgctat ctcttaatag ctggtggtct
    21661 atattgagta ttctgttctg catatcaata tgcgttgtca gtccgcaaaa ttcagaaata
    21721 cgaagtcccg tttccagaag aagcacaacc tcatcataat acttgctgta aatcttatcc
    21781 gtttccataa aggcaagcag gcgttcttcc tgctctggcg taagaataac tttcggctcg
    21841 gtatcatctt ccagaacatc acttagctta aattcaaacg ggttctttct gatacagtcg
    21901 tcttgtatcg ccatataaaa tgacgctttc aaggaacgct tatagttgtc aatcgttttg
    21961 taggcatatc ctctttcact cattctgata gcccactctt tagcgtccga ctgtttgacg
    22021 gtatcaatcg ctctcatacc caatgggtca tttttcagag cgttcataag atactgtcgt
    22081 cctttttccg tagctcgctt gatgttcttt ctttggctgt tcttcttgtc gtaaagctgg
    22141 cagactgtca ttttaccgcc tgccgtgtca ataccgtcat caaggtcttt ttttatctgt
    22201 gcttcttttt ccctcaacga tatatcatca cgttttccag caggcgtttt gtctgtcggt
    22261 acaagtttcc aagcataaat aaactgtggc tttccgtata tatcggtata tttataaacg
    22321 tatcttccgt cttttcgctg gctctctcca ttacgcaaat tgcgattttt actgtctttt
    22381 cgtttcacat tagacatagt gtaaagctcc tttccgtcat ggaatgagcc gtgatacgct
    22441 atacttatta taccatatcc acggctctat gacattagat ttcgtccaat gtatctatga
    22501 ttttttcaaa ttgttttcgc tttatctgaa tacggttgcc gttcacgata acccagccag
    22561 cgtcaatatt ttcttcggct aatttacgca acttcttttc tccaatgcgg aagtatttgg
    22621 acgcttcttc aatcgtcagt gtatattttt cccagatagg cacatcagta ttattcataa
    22681 tttatgcacc ccctttactg tgtgtcagtc tataagttat ataaacaggc agacggcttt
    22741 ggaaagctcc attagtattt caactattcc cattcttgtg ggcagaaccg caccatgcgg
    22801 aagtatcatt atgctgtaag ggtaggtcat ggcaaaacga cttttccaac ggtcgctctc
    22861 ggatcactgc gtgggtcgct cgctttcttt tggaaaggtc atggcgtaca gttcccgtgg
    22921 ctctctatcc ttacaaacgt atctgtctgc tttattcagt tttcaaagag ctgtggcgaa
    22981 agcggtatgc tttcatgtaa aagcaggagc agagcaggaa agaggggatt gaacaaatca
    23041 tgctctgcgt tactgctggt tattcttctt caatttctaa ggcaatatca aaccctaaaa
    23101 tcattttgat aagtgcttct cggataagtc cttttaactc catatctaca acgatataaa
    23161 cattaccgta ttcatcatag agtggacgca aacagcactt cgatatgtaa gggtcataaa
    23221 atgcaagtac cttttcaatc gctttttcgt ccccgtccat agcttgtgaa agcaagtagt
    23281 aagacggctt cttaaatgtg tgtttcattt gctatcgttt ctcctttaat agttttctca
    23341 taagtgccaa tgaattgtgg cggttctgaa aaactccgct tctggaaatg ttttgctttt
    23401 tggcgatttc ttcattgttc atttccaaaa agtaatacat caacaaagtt tctcggttac
    23461 gttcggataa ctgtttcaag gcttctgcca attcatcatc atagacacga atatcattac
    23521 cgcatacatt gaaaatcgta tagtctgttt cgtagtcgtc ccatacagcc aagttttcaa
    23581 ccatgatttc tggaagttca caaaaggaaa tttctttttc ttttcttcgc ttcaatccct
    23641 gctgatagtc cttgacaaca tgacggacaa ctttcttcaa acagctttca aaacggcatt
    23701 gaacagtttt ctggaagtca gacggtttca tcaatctcac cccctttccg ttatgacgtg
    23761 aaaagtgttt atccctcttt ccgcaccata tagggaatgg caagtgtgat ttgttaagtt
    23821 gaaaccagaa aaaatcgaaa aaacttttta gtaaaacaaa aacagcccgt gtgaatgtga
    23881 atacacaaac ggactgcttt ataaatgcta tctgtatgaa tttttaactc ttgtagaggg
    23941 tagaatgatt gtaatactga actaaaatat tttttcagtt gggaacagca aagtattatt
    24001 ccatatacaa tcatggcgta gcccccttta ccagagctat accttgtgcc accaatgaaa
    24061 aaggacggct ctggctatat ccaaaaccgc cccgttatgt tttcaagacg cacaaaagta
    24121 tatcatctcg gaacggttat ggtgatttaa aatgagaaaa acaaaagaaa cacatacttt
    24181 tgatttcaga ccgctagggc tggcaatcag agaagcccgt gaaaaagctg ggctttctcg
    24241 taatgattta ggcgataagg tattctatgg ggaa
//
LOCUS       DAMJWS010000067        24125 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMJWS010000067 DAMJWS010000000
VERSION     DAMJWS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.067"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            384..896
                     /locus_tag="PC399_002646"
     CDS             384..896
                     /locus_tag="PC399_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593499.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KMDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            complement(1349..2446)
                     /locus_tag="PC399_002647"
     CDS             complement(1349..2446)
                     /locus_tag="PC399_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2593500.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            2739..4265
                     /gene="glpK"
                     /locus_tag="PC399_002648"
     CDS             2739..4265
                     /gene="glpK"
                     /locus_tag="PC399_002648"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2593501.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            4306..5421
                     /locus_tag="PC399_002649"
     CDS             4306..5421
                     /locus_tag="PC399_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2593502.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(5702..6466)
                     /locus_tag="PC399_002650"
     CDS             complement(5702..6466)
                     /locus_tag="PC399_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2593503.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            6725..6815
                     /locus_tag="PC399_002651"
     tRNA            6725..6815
                     /locus_tag="PC399_002651"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6759..6761,aa:Ser,seq:gct)
     gene            6903..7691
                     /locus_tag="PC399_002652"
     CDS             6903..7691
                     /locus_tag="PC399_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF2593504.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLTFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            7712..8452
                     /locus_tag="PC399_002653"
     CDS             7712..8452
                     /locus_tag="PC399_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2593505.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            8628..9002
                     /locus_tag="PC399_002654"
     CDS             8628..9002
                     /locus_tag="PC399_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593506.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            9094..10554
                     /locus_tag="PC399_002655"
     CDS             9094..10554
                     /locus_tag="PC399_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2593507.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            10607..13636
                     /locus_tag="PC399_002656"
     CDS             10607..13636
                     /locus_tag="PC399_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2593508.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            13713..15779
                     /locus_tag="PC399_002657"
     CDS             13713..15779
                     /locus_tag="PC399_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2593509.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    16162..16372
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            16598..17413
                     /locus_tag="PC399_002658"
     CDS             16598..17413
                     /locus_tag="PC399_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2593510.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            17441..18118
                     /locus_tag="PC399_002659"
     CDS             17441..18118
                     /locus_tag="PC399_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2593511.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            18177..18899
                     /locus_tag="PC399_002660"
     CDS             18177..18899
                     /locus_tag="PC399_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2593512.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            19547..20698
                     /locus_tag="PC399_002661"
     CDS             19547..20698
                     /locus_tag="PC399_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF2593513.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRLSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            20744..21919
                     /gene="thiI"
                     /locus_tag="PC399_002662"
     CDS             20744..21919
                     /gene="thiI"
                     /locus_tag="PC399_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF2593514.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            22075..22878
                     /locus_tag="PC399_002663"
     CDS             22075..22878
                     /locus_tag="PC399_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2593515.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFSQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            23121..23762
                     /locus_tag="PC399_002664"
     CDS             23121..23762
                     /locus_tag="PC399_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2593516.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         9583 a         2470 c         4339 g         7733 t
ORIGIN      
        1 tagaatattc agtaaaatga ttttgatata ctctctatat tttgtatgtt aaaagtaaat
       61 aaattattgt taaagaaggt aaagactttt aaatttctta tcataggatt ttaagtaaat
      121 aagaaggatt ggagtaatta aaaattttta ttttgcaaaa ataagtattt atttatatgg
      181 ataaatttga ttttaaatct ataaatatag ataaaatcta attatttaac ttcaactatt
      241 cctcaaaaaa gcaactttta aggaatagtt gaagcaaaaa gtttggttac ataatagtaa
      301 ttttagttac aagaatgaaa ctgtaaaact taggattttc tttatataat ttcattgaga
      361 acatttaatt taggaggtaa gatatgaaag taaaaaaatt taaaagtggt atagtaatgg
      421 cattaacagt aatggcaatt ggagcatcat tgacaaatgt atcagctatg gaattaaaca
      481 agagtaaaga gtttgaaact aaatacaatg aaatgaagat ggattttcaa gaggaatatg
      541 gaaaagaaga caaatttaat ataacattgg atgatgaatt tacaaaagaa gaagtaaatg
      601 gagctaaagg gattataaag acaactaata aaatgaataa tgaggttgta gtattaaatt
      661 attttgatga aatgaaatct ggaattgaat ttgagttgaa gccaaaagca gaaaaaaatg
      721 caattttgag agatgaattt caacaaggtt atggaaagga agataaattc aaagttgtgt
      781 tagatgatga atttacaaaa gaagaggtaa atggagctaa gggcattata aagactacag
      841 ataaagagac aggtgaggtt atcgtatata attactttga tgagcttgaa aaataatgct
      901 taaagattac tttgattgaa tgaaaaggta attgtgttga gaatataaaa tgactgaata
      961 aaaacagttg tatcaaaaca aacaataata gaatgtaaga atgattgtgt tgaaaatatt
     1021 aaatgactga ataaaaaacg attgtgtcaa aaataaaaaa taattaagtg taagaatgat
     1081 tgtatcaaga atataaaatg actgaataaa aaacgattgc gtcaaaaata aaaaacaatt
     1141 aaatgtaaga ataattatgt caaaaatata aaataaatta aaagaaaaag attatattga
     1201 aggaaaaaga tttgaaatag gggtgaactt tacatattat agttcaccct tatacaaaaa
     1261 tataaaatat aatttattat aggaaaattt aaaaaaatca gtctatttaa tttatactga
     1321 tatattgtag gattttacaa acaaactgtt atataacttt ttttatctcc aattttgatt
     1381 ttaagatatc taagaattta taacaatctt ctggatatat ttctcccata tttgctactt
     1441 ggaatatatt agcatctagg aatttacctt ttcctggata gacaacatag ccactttccc
     1501 tttctagccc taatataaac tcatttacat tgatttcttt aggtagaaat acagatgtta
     1561 ctgtattaga catatactta tctggaagta aaaatgttag acccaattct tccataccat
     1621 tccttaaaat agcagaacat tctttgtatc tttctatttt actgtcatct tccaaaaact
     1681 cagttaaagc ttcattaagg gcaaatatta aagtaacatt aggtgtattt ggtgtttgag
     1741 aagatgattt agcaacttta tagtgtttat aaagattaag atacacattc ttacattgat
     1801 tttctttaat attttgtaaa atactctctt tagcacaaag gtatgcagaa cctggaaatg
     1861 ctccaagagc tttgccacca acacctgtta ttatatctat attaaactct tccacatcaa
     1921 tatattcacc tgctgaggca cttattgcat ctacaaaaaa tattttgtta tacttcttag
     1981 caagttttcc tagttcaggg actggattta tcattcctga actagtttca tggaacacca
     2041 tagcaattac ttttatatca gaattttcaa ttatgtattt ttctaaaatg tctaggtccg
     2101 ggtattctgc ccattcaaaa ttaggtttat atgtttttac attataagta gataatattt
     2161 cttctagcct ttctccaaaa acaccattac taactagcag aactgcatct tctttgtcaa
     2221 agatagaaga tagtacacat tcatttacag atgttccaga gccagataca attaatgagt
     2281 agtaattaga agttgcatta tatagtttat tgattttttg ttgtgtatct ttgaataaat
     2341 ccataaactc atttcctcta tggcatatat catagtgaag aagtgaattt ctgactttat
     2401 ctgtaaccat tactggtcca ggactgaata gatatgtttt tttcattgta aagcctcctt
     2461 aaaaagttaa atttaaattt ttgaatgact gattatgtgt aaatattaaa atattatgtg
     2521 ttattttaac tagattatag aataaaataa taaatattgc aataatattt caataaaaat
     2581 acttttttaa tcactataat taaaaataac aaaaaatatt gtaatgaata ttgcataaat
     2641 ccacatgatg atataaaata taaataactt gaatagatta tatattcaaa aaataaaaaa
     2701 gaatttatta attctgaaaa taaaggggga catttaaagt gaaatatgta ttagcaattg
     2761 accaaggaac aacaggaaca agagcagtat tgataaacga aaaatcagag tttgttttta
     2821 gcgattatat ggaacatact caaatatatc cacaatctgg atatgttgaa catgacccta
     2881 tagaaatttg gaataataca aaaactgtga gtaataatgt attacaacag gcttttgaaa
     2941 atggaatcaa tgaaagtgat ataaaaggaa ttggtataga taatcaaggt gaaacagtaa
     3001 tgttatggga taaaaataca ggtaaaccac tttacaatgc tattgtgtgg cagtgcagga
     3061 gaacttatga ttatgtggaa aatttaaaga atataccagg tttagaaaat aagataatag
     3121 ataagactgg gttaattata gacccatatt tctcaggtac aaaaattaaa tgggtaatag
     3181 ataatgtaaa aggtgtaaaa gaaaaaatta aaaatggaga agtacttgca ggaacattag
     3241 atagttggtt aatatggaaa atgacaggag gaaaatcttt tttaactgat gtatctacag
     3301 cagcaagaac aatgcttttt aatataagag atatggattg ggatagagag ttgcttgaca
     3361 ttataggagt acctagaagt attttagcag atataatgcc tacgagtggt gattttggaa
     3421 ctacagataa ggaggcattt tgtggggttt ctataaaaat tactgcttca atggtagacc
     3481 aaccagcagc tttatttggc aatggatgtt ttgaaccagg aatggttaaa aatacttatg
     3541 gtacaggatg tttcctatat atgaatatag gtgaggaact aaatatagca ggtaatggta
     3601 ttttgacaac tgttgcttgg aaaataggag ataaagttac atatgcttat gatggtggag
     3661 tgtatatagc aggtgctgca atacaatggc ttagagatgg cattggagtt ataaaaaatt
     3721 attcagaaac tgatgacatg gcaaattcca tatcaagtac tggaggtgta tattttgtac
     3781 cagcatttgc aggtatagca gcaccttatt gggaccaata tgctagaggt acaatggttg
     3841 gaataacagg aagtactaaa aaagagcata tagtaagagc gactcttgag tctgttgcac
     3901 ttcaagtcaa agatgttgtt gattctatga acttagcctc agggaaaaaa ataaaaatgt
     3961 tgagagtaga tggtggaatt actaaaaata actttacaat gcaatttcaa tccgacatat
     4021 taggtatacc aattgaaatt gcagaaaatc ctgagacgac agtacttgga gtaggatata
     4081 tggcaggtct ttcttgtggt gtctggaaca gtattgatga aattagaagt gaatttacaa
     4141 taagcaaaaa atatattcca aatatgacta ctcaaaaaag agaagaaata ttacttggat
     4201 ggaaccaagc agtaaaaaga gctatgaatt gggaaaacga taccaaaaag gtgtgtgcta
     4261 attaagttac aatagagtat tttacatgta taggaggaat taaaaatgcc ctttgataaa
     4321 attatattat acataatggc tgtgggagtg cttttaggag cgttagatag aataattgga
     4381 aataaatttg gacttggcga gcagtttgaa gaagggttta attcaatggg tccattggct
     4441 ttaggaatgg ttggtatagt ttgtttagct ccagtaattt cagatgtact aggtcctata
     4501 ataataccaa tcttcaatct ctgtggagct gacccatcta tgtttgcaac tattttagcg
     4561 aatgatatgg gagggtatcc attggctatg gaacttgcac acaataaaga ggcaggaatg
     4621 cttgcaggat tggttgtgtc atccatgctt ggatgtacat tagtattttc aattccagta
     4681 ggactaggtc ttatagaata tgaagacaga ccattctttg caaaagggtt actgataggc
     4741 ttgataacaa taccttttgg aggtattata ggaggattaa tagcaggatt taacttatca
     4801 atggtaatta taaatatgat accagtgata atattggcag taatattggc agtaggttta
     4861 aaatttaatt ctacgcttat gataaatggg tcattagttt ttggaaaatt tatatctata
     4921 ttgataacga taggtttggg agcagcagca tttcaagaaa taacaggtat agtacttata
     4981 aaaggcatga cacctataag agaaggtatt caagtaattg gaagtattgc aatcgtgtta
     5041 ttaggtactt ttccaatatt acatttatta gttaaagttc ttaataaacc acttacaata
     5101 gtgggtggaa aattaggtat ggatgccact agtgcagcag gattagtttt tactttagcc
     5161 aatagtattc cagtatataa aatgatgaaa gatatgagtc caaaggggaa agtagtaaat
     5221 actgcttggc tggtttgtgc tacagcagct ttgggggacc atttaggatt tacagcagga
     5281 gtagttccag agatgattac acctgttgta attggaaaat tggttggagg agtattggca
     5341 attgcacttg ctatacttat gacaaaagat ttaaccttgg aagaagaaca aagtattaaa
     5401 atgaagaaag atgcagtgta gaataaacaa tacttaagtc aaatttaaat tattttaaag
     5461 taggagttta aataactaaa aataaattaa aaagacagac aattaaaaaa taatcgaata
     5521 actaaaactt ataaattaaa taaaatggca atcaaataac taaaaatgat aagctaactg
     5581 aaagtagtta cataattaaa atataaagta tagtttagac ataaatggag ctaaataaat
     5641 aattatttgg ctccttaatt ttaatttatt acatcaagga actaaaaatc aatcatataa
     5701 gctaattatc agctatgact aaaggtattc ctttttgaga aaactctatt attatattat
     5761 ggtctatact agcagaggta ataatagtat ctatatgttc tataggagct actgatgcaa
     5821 atgaatttat accaaactta ggaggttcga caactactat agattttcta gacctttcaa
     5881 tcattttttt tctaacaagt gcttcttcta agtgatagtc agagattcca ttttcaagag
     5941 tgatacctcc agcgcctaaa atagctttat caacattaaa ttgttcaata gctgacagtg
     6001 taagcggacc agaaaaagaa ccttcagttt ctctaagata gcccccaagc acatatactt
     6061 ttatattagt attcataagc tcgtttgcaa ctaaaattga gttggttatt acagttagat
     6121 tctttttatc tttcaaattt tttgaaactt caagtacagt agttccagta tccataaaaa
     6181 tagtatcatt tgtatttata aactcactac atttaatagc aatctgtttt ttatcgtttg
     6241 agtttatttt ttttctttta acatagttaa gttctacgct acttgctata actggtatag
     6301 caccaccata aactcttttt aaaagaccga tactctccat atgggctaaa tctcttcgtg
     6361 cagtttctat tgatatatca aacatttgaa ctatgtcatt tattttaatt atattatctg
     6421 atttgagaat acttaaaatc ttctcatatc tttcctgtgg taacataaaa tatcctcctt
     6481 ggactagtat tattttagtt ttggcataac ttacaattaa gtcagagttt aggtatattt
     6541 gttaatgtat gtatatttat caaataatat tataagtgtt atgcaagata attttaccat
     6601 atttaaaact tacatttaag caaaaaagta aaaatagatg tttaataatt caaaataact
     6661 caaattttat aaaacaattg actaaaatat gattttatta tataatttac tagtatagag
     6721 atatggagaa gtactcaagt ggctcaagag gatcccctgc taagggatta ggctgggtaa
     6781 ccggtgcgag ggttcgaatc cctccttctc cgccaattat taaaggctat ttttattaaa
     6841 gatagtcttt ttataatgtc ataaatgatt agatatgtat aactaaagaa aggagggaat
     6901 agatgacaac tttaattttt ttcatagtgg ggatagcatc tttggtagtt gcttttgtta
     6961 ttgatggggg acatgttgga gcattatttg tattttcatc agcacttgtt gtgtttggtg
     7021 gaactatagg agctgttgga gcttcgactc cattccctgt gtttaaaaag tctataaaga
     7081 taatgttatt aacttttaaa aataaaaagc ctaataacat agaaaatata atctatttca
     7141 aagaaattgc aacaaaggct aggaaagagg ggcttcttag tattgacaaa gaaataagtg
     7201 agaatgatga attagaccct tttattagaa aagggcttga acttatggta gatggagtgg
     7261 atgcagttac tataagaaat atattggaat cacagataca gataacatca aaacgacata
     7321 aagaaggaat agcaattttt gattcagcag gtggatatgc acctacactg gggataatag
     7381 gtacagttat gggattagta catattttag gtaatctttc aagtaatcca gatgcattag
     7441 gacctcaaat ttctgttgca tttttggcta ctttgtatgg tgttggttct gccaatttaa
     7501 tatggttacc aatagcatca aaacttaagg ctataaatca acaagaagta agtgaaagac
     7561 ttttggtgat agaagcaata ttatccatac ttgagggtga taattctaat ttaatgacag
     7621 aaaaattaaa aatattttta aattcaaaag aactaattga acttgaagaa attggtggta
     7681 gagctgaata atataaaagg atggaatgtt tatgttgtat gatgaagatg aaaaagaaga
     7741 aaacaatgag cgatggcttt taacttattc agacctcata acattactta tgattttctt
     7801 tgtaataatg tattctatga gtaatgttga tgcggagaaa tataaacaat tatctcaatc
     7861 tctaaattct gcttttggtg gttcttctgg agttattgaa ggtggtaata gcaagataga
     7921 accagtagtt gaaccaggaa gtaatgattt agatacttta caaaatgcta agtttaaaaa
     7981 aattggtgaa gaaattcaga agtatttaaa cgaaaatggt atggctaatt cagtatcatt
     8041 aagagttcaa gatagaggat tagtaataag ccttaaagat acaatactat ttgatacagg
     8101 gaaagctatt gtgaaggata attctagaga taagattatt caaataggta agatgttaaa
     8161 tgaaatgaat agttacatga gagtagaagg ccatacagac aatatgtcta taaaaaattc
     8221 agagtttaag tcaaattggg atttatcagt tatgagagca accaatgttg ttcaacttct
     8281 tattgacaat gcaggtatag caccagataa attatctgct gtaggatatg gtgagtttag
     8341 accaatagca gaaaattcat ctgaagaagg taggtcaaaa aatagaagag ttgatatagt
     8401 tttagttgat agtaaatatg ataatgtaga aaatgtttca aaggataaat agttaaatta
     8461 gctgtctcaa tacagaatta attctaaagg agatggcttt tttatgcgaa aatcaaaaat
     8521 agatacatag taagttacct ttttgtagga atatatggta tcttaaatat ataatattta
     8581 aataatgtat aagctcaatt aaaaaattta tagaggatgg aaaataaatg ggaaataaaa
     8641 ataaaaatac taaatataaa gataaaacaa gagaaaggta taataaaaaa gttgagaatt
     8701 taaataaaga aaaattaaat aaaattaata agcaatctaa gaaagtagat aagattgatg
     8761 atattattaa taaatctagt attgtattat gtgtacttct agtagtgttt tcattgcttt
     8821 acttatttaa actggttcaa cttaatataa taatgatact tttagtaatt acagtagcta
     8881 ttcaacaagg attggtgtca tttggtgcat ttagaaataa aaattatgta aaagctttat
     8941 tttctatatt tgtaatagta tgtttcttaa ttgcttttaa atatgttgta aatcctgttt
     9001 aaatatatca ataaacgttc tataattaat ataaatagtg aagttacagt tttaatgcta
     9061 ttataaattt tataatttga aggagtagat tttatgaata ggtgtaagtt tgcaaaatta
     9121 attatcacat caatgattgt tttattactt acaggatgta gtttaaagaa ggaagataat
     9181 tataagttag acccagaaaa tccgacatca atacaaattt ggcattatta taatggacag
     9241 cagaaaatgg cttttgataa gcttgttaag gaatttaatg acactgtagg tgctgaaaaa
     9301 ggaattattg ttgaagcatt cagtcaaggt aatgtagttg atttatcaga gaaagtttta
     9361 gattctgcaa ataaaaaagt tggttctgaa gatgtaccaa atatatttgc agcatactca
     9421 gatactgcat atgaaatgga taaattaggg ttactagtag atatggataa gtacataagt
     9481 aaagaagaat taaatgaata catagacgaa tatgtagaag aaggcagaat aaattctaag
     9541 gatgagttta aacttttccc aattgcaaaa tcaactgaaa ttttgatgtt aaataaaact
     9601 gattgggata aatttgcaaa agctacaaat tcaaatataa atgatataaa aacaattgaa
     9661 ggattagtag agacatcaaa aaaatattat gaatggacag atagtttaac gaaagagcct
     9721 aatgatggta aagcattttt tggaagagat gctttagcaa attatattat tataggaagt
     9781 aaacaactag gagtagaaat tttttctgtt aaagatggta aagttacact aaatattgat
     9841 gaaaaagtta tgaaaaaact atgggataat ttctatgtac catatataaa tggatatttt
     9901 gcttcttatg gtaagtttcg ttctgatgat gcaaaaacag gtgatatatt agctcttgta
     9961 ggttctagtt caggagcagc atactttcca gatagagtaa tgctaaatga taatgagagt
    10021 tataatattg aaacatctgt ttttcaagca ccacgtttta aaggagaagg aaaatatgct
    10081 gtacaacaag gtgcaggaat ggctgtaaca aaatctacta aagaagaaga atatgcatca
    10141 gtagaatttt taaaatggtt tacagataaa gaacctaata taaaattctc tattgcttct
    10201 ggatacttac cagtaaagaa aaaaacgaat gatttggatg ttataaaaga agttatgaag
    10261 aaagataatt tgagtttatc tgataaagtt gaaggttcaa tagcctcatc tattgaacag
    10321 attaacagtt gtaatttata tactaataaa gcatttgatg gtggaactaa agctagacaa
    10381 atacttgaat attcaatgct tgataaagct aaagaagatt caaataaaat aaatgaatta
    10441 gtaaaaagtg gaacttctaa gaaggatgca gtagcacaat ttaataatga taaaaatttt
    10501 aaaacttggt ttgaaagttt taaggctgag cttgaaaatg ctataaaaaa atagatgaat
    10561 tggcagttag tgtgttgacg agcacaaagg tataggtgga atgggtatgg aaaacaatct
    10621 aagaaaaaac aattcaataa tgaaaaagat gatatttccc ctaattattg ttatgctaat
    10681 tcaaacaagt ttattttgtg ccacaatttt atggggagga actattaaga aattaaatga
    10741 taattcgttt gacatcttaa atgaaagagt aattaatcgt aaaaattata tacaaaatga
    10801 aatgttacaa agatggtcaa atacaagtga aacggaagct acaattaatt cctctgtaaa
    10861 aaaagtatta aatgaaaaaa atgccttaat tgaggatgta ggcaacaatg aagaaattaa
    10921 caaagatatt ataaaaagca tatcaaatga tttaatatat cttttgagaa aaaattctgt
    10981 tacaggagct tttataatcc taagtgacaa aaatattaac gataagtcaa tagatagtaa
    11041 aggtgaaata agtaggacag gattgtatat aagggattta gaccctaagt ttaatccaaa
    11101 tgataattct gatttgttaa ttgagagagg ttcatcagat atagcaagaa gttttggaat
    11161 atctatggac agttattggg aacctaagtt ttcatttaaa agtgaaaatg agcaagatgg
    11221 agatgatttt ttttataaac catttagagc tgcaattgat aatccaaatg tatcaagttc
    11281 taatttagga tactggggaa gtaagttttt attgacaaat gcaggtgata ggcttatgac
    11341 atatacagtt ccccttattt atgaagatgg aactgtatat ggagtacttg gaatagattt
    11401 aagtgtaaat tatttaagag aattacttcc atactcagag ataaatggaa ataaaaaggg
    11461 taattatatt ttaggtgtag atagaaataa tgatatggcc tttgaaaatg taatttcaaa
    11521 tggatactca ttaaaagaaa aattttggga tggtttacat tctaatttag agcagaagga
    11581 atcgtataaa aatatatata agattaaaaa taaagataat gaatgtggta atggcaacat
    11641 ttatgctagt gtacaatact tggatttata taactccaat actccatttg aaaatgaacg
    11701 ttgggctctt ttgggtatta ttgaagaaaa aagtcttttg tcattttcaa aaagggtaga
    11761 gaaattagtt ataatatcta tagcaatttc tttattaata ggtctaatag gtatttacat
    11821 tgcatcaaga ttatttacaa aaccaattgt tcttttagcc aacaaagtta gaaacagtaa
    11881 tccatcaaaa ccagtaaatc ttgataagat aaatatatct gaaatagatg aattatcttc
    11941 agctatagag ttgctcagtg caaatgttgc agattcttcg tcaaaattat cacagattat
    12001 tgaaatggta gatatgccaa ttggggcttt tgagttatac aaaaacgcag agaatgtttt
    12061 ttgtacaaaa gaatttttta gcgtgtttac tactgaaatc aaatttgaag aatatggata
    12121 tatggaaaaa aataagttcc tgaaaaaatt aaggggacta gataaatatt tgcaagaaag
    12181 ttatgaaaca gaaaatacat atatatataa gtttgaatct aaagataaaa aacttaagtg
    12241 gataagaatg aatatctttg aagatgagct aaaggttctt ggtgttgttg ttgatgtcac
    12301 aaaagaattt attgagaaaa ggaagataga atatgaaaga gattttgata ttttaacgaa
    12361 tcttttaaat aggagagctt ttcattcaca gatacaagaa aaatttaaga ataaagatga
    12421 acttaagata gcagcattta ttatgtggga tttagataat ctaaaatata ttaatgatac
    12481 atatggacat gatgctggtg atgaatatat taggtgtgct gcagacatac ttaagaagtt
    12541 tatatcatgg aatggtatag tatctagact ttcaggtgat gagttttatg tatttatata
    12601 tggatatgaa agcaaagata gtataagaga tattattaat tctgttaaga aacagatgaa
    12661 taatacctta ttaaaacttc atgatggaac agaatttaaa ataagggcat cagcaggaat
    12721 ttcctggtac cctgatgact caaccaacta tgatgaatta atgagatacg cagattttgc
    12781 tatgtatcaa attaaaaata caatcaaggg tgaagttagt gaatttgata ttaatatata
    12841 taataaggat tcattcttat tgagtaatag agaagaatta aacaagttaa ttgatggtca
    12901 attagttgag tatgcatttc agcctattgt agattgtaaa ggtaaagtat ttgcatatga
    12961 agcacttatg agacctaaaa taaaaacttt tagttcacca atagatgtca ttagattagc
    13021 tcgttcacag tctaaattat atcaaattga gcgtattaca tggtttaaag cacttaaatc
    13081 atttaaaaaa tacaaagaaa aatttggcga ttgcaaattg ttcttaaatt ctattcctaa
    13141 taataatttg tcaaaagtag attttgagga aattgaagat ttatatggtg aatatttaaa
    13201 tagagtagta ctagaaataa cagagaatga taagattaat gaagatttta ttgaaaaaaa
    13261 gaaagcatat atagaccatt ggaatataga aattgcttta gatgattttg ggacaggtta
    13321 caacggagat gctgtactat tatatataat gccagactat gtaaagattg atatgtctat
    13381 tgtaagaggt attgataaag atgaaaacag acagaaaata ttaaaaaatc ttatctcata
    13441 ttctaacgaa agaaatatta aagtgatagc agaaggtgta gaaacaaaag gtgaaatgga
    13501 aacactaatt aatcttggag tagattatat gcaaggctac tatattagta aaccatcatt
    13561 tatacctgaa gatataagcg acaaaattaa aaaagaactc aagatatgtc atgaaaatag
    13621 gaatgatgtg gtttaataga ggaaaaatta aatttatttt tatgaacttt tagtttataa
    13681 ttaaaaagaa aataatataa ttataaatca aggtggtgga gctaataatc acaacaaata
    13741 tagatataag aaagttaaat gaaaatcaat tagaagcagt tgagcatata gatgggcctt
    13801 gcatgatact tgcagggcct ggttcaggta agaccagagt aattacatat agaatagcca
    13861 atatggttgt aaacaaaaac atagcaccaa caagaatctt ggcaataagt tttaccaagg
    13921 catcttcaat agaaatgaaa aatagagctt tgaatttaag tgatgacata agacttaata
    13981 aggtaaccta tggtactttt cactctgtat tttttaaaat tctaagatac tttgaaagat
    14041 acaacttaga cagtatattt gatgaaaagt caaagcgaat gacaatcaag gcaatcctaa
    14101 aaagtttaaa tatagagaat gctgatgatg atgagaatat aggacaagtt ataaatgaaa
    14161 tttcctatgt aaaaaatgaa cttatggata aaaatgaatt taattcagaa gtattaacaa
    14221 aagatgagtt tttaaaagta tacaacttat acgaagaaca aaaatctaaa gttaataaaa
    14281 tagattttga tgatatgctt ataagaactt attatttact tttgaacaat aagtctgcac
    14341 tagaaatggt tagaaatgtg tataaatata tattgataga tgaatttcaa gatataaata
    14401 aagtacagtt tgaagtttta aagttaattt gtagcccatt aaacaatata tttgcagttg
    14461 gagatgaaga ccaaagtata tatggattta gaggtgctag accagatttt ttgcttgaat
    14521 ttgaacaata ctttaataat actaaaaaaa taatattaga tataaattac aggtctaaaa
    14581 gtgagatagt gcatactgca aatagactta tcgacaaaaa taaaaataga tatgaaaaaa
    14641 tcattaagtg tagtcagggg gatggaggaa gtgttaccta tatttctcca catgattcag
    14701 aggaagaagc cttatatatt gccagggaaa taattgacga aatacaaaaa gattatgtag
    14761 agtattcaga ttttgcagta atttatagaa ctaatataca gtcaagagca ttagtagatg
    14821 tatttatgga tatgaggatt ccatttgtag taaaagactc agtaattaca atatatgacc
    14881 attgggcaag tcaagatata ctagcctatt taagaatagg tattaatcca aaatcaaata
    14941 aagattggtt gagaattata aacaagcctt ttagatatat atccaaagac agtataaata
    15001 tggttaagga tgaaaaagac tttataacag cattaataaa taagtgtaat cttcatccta
    15061 aacaagttaa aacaataaat gatttggaaa tagatttaag ttatttaaat actttaaatc
    15121 ctaaaaatgc aatatcttat attagaacta gtcttgatta tgatagatat gttttagatt
    15181 attgtagcaa cagaaagata aagactaatg gacttgtaga gatattaaat gaacttgaga
    15241 gttcagcaac taattttaat actgttacag agtttttaga gcatattgat agagtaaaga
    15301 ctgagttaac ggaaaataac aagaataagc agactgaggg tgtcatattt acaactatgc
    15361 atagtgcaaa aggattagaa tttagaaatg tttatattat aggtgtaaat gaaggaacaa
    15421 tacctcatga aaaatcatat gatatttgta aggaagagaa aaaagaagaa caattagaag
    15481 aagaaagaag acttatgtat gttgctatta caagagctga agaaaagtta tgtataagct
    15541 ctactttaaa taagtatggt aagaaagttg ataaatcatt atttattaat gatataaaat
    15601 ctccaacgaa aaaagaaatt gacagtattg gtgtagggga taaggtatac cataaaaaat
    15661 ttcatgaagg agaaataatt aaaaaagatg gaattatgtt tacaataaag tttaaagata
    15721 gagagcgaat cttggatttg aaaacttgtt tattgaagaa tataatttat actatttaat
    15781 ttagaatcta aatttagata gaaatataat gtattattta ttatgtgcaa gacaaataga
    15841 tatgtgtaaa atgtattatt taacttatta gaaaaagaaa taagggttta atatattatt
    15901 tatagttgtt tagaaaaagc aacaatgtgg aaaatattat ttagtattat taaagaaaag
    15961 caacaatgtg aaagatatta tttagtatta tttaagaaat agctaaaaat atgaaatata
    16021 ctatttataa ttacacaaat aatatcaata aatataaaca gtagtattta gataatttac
    16081 aagtaaaaat tattaaaaaa ataaaaaact tattgacaaa aatattataa ggaagtacaa
    16141 ttgtattaac aatttaagga aacaaatcca atgatagaga aagtagcata tatggattta
    16201 cagcgagtta ggggttggtg taagcctaac aatgaagtct atgtgaagag agctctggag
    16261 gagattatca gaaactttag tatgataatt ggtttacccg ccttaaggga ttgagaggat
    16321 aagtacttac ttatcaaaaa gagtggtaac gcggatataa ttcgtctctt agctgtaaag
    16381 ctaagggact tttttgattt taaaataaaa atatccatac agcttaaaag ctaacagtca
    16441 caaattttga aagtaatata agtatttatc aataagagtg gtaacgcggt aaagtcgtct
    16501 cttagtcatt aaagactgag agactttttt tgtaactcga aacatttaag tattaaaatc
    16561 tgagggaaat tataattaaa agagaggggt agttaaaatg aaagtattta aaaaattatt
    16621 aagtttagga ttagtattag gattgacatt atcgctagta ggatgtagtg gtggaggaga
    16681 aaaaacaaag ttagaacaaa taaaagacaa tggaaaatta gttgttggta ctagtgcaga
    16741 atttcctcca tttgaatttc ataaggtagt agatggaaaa gattcaataa aaggattcga
    16801 tataatgttg gctgaagaat ttgcaaaaga gcttggtgta aaggttgaaa ttaaggatat
    16861 gtcatttgat ggattaatag gtgctttaaa tgcagaccaa gttgatattg ttcttgcagg
    16921 tatgtctcca acaccagaga gagaaaagag tgttgatttt tcagaattat attatttaag
    16981 tagaaatgca gttatagtta aagatgcaga tatagacaaa gtaaaaactg aagatgattt
    17041 aaagaaactt agagttggag ttcaagcagg aagtatccaa gaagaatatg tagttaacac
    17101 tttaaaaatg acaactacaa aatctttgaa agcaatacct gatttaatta cagaattaaa
    17161 aaatggaaac atagatgcag ttgttactaa tgaagcagtt tctttaataa atgttaaaaa
    17221 atatgatgga ataaaaatgg caaatactga agttgggaaa gatgtaacag aaggtatggc
    17281 agcagcaatc aaaaaatctg acaataacaa agattttata gaattattaa acaaaaagat
    17341 aaaagaatta caagatggca agaagataga agaattttta aatgaagcat ctactgaagc
    17401 tgcttcaaat tagtaagaaa gttatgtggg agatgaaatt atgagtttag attttagttt
    17461 tttaagtaga tttgggactt cttttttgga aggaacaggt gtgacagtat caatttctct
    17521 tgtggcatta tgctttggat ttataatagg tataattatc tgtatggcaa aaatatcaaa
    17581 gagcaaagta ttaagagcaa tatcatcaat ctatattgag gttttaagag ggacaccatt
    17641 attagtacag atatatatag tatggtttgg tttacctcaa ttaggaataa gattccctat
    17701 gttgtttggt ataccaagtg aatttatagc tagtgcattt gcattatcag taaactctgg
    17761 tgcatacgtg gcagagatac ttagatcagg tatacaatca gttgataatg gacaaatgga
    17821 agcaagtaga tcattaggat taaattattg gtcaactatg agatatataa taatacctca
    17881 agctataaaa aatatattac cttcattagc aaatgagttt attacacttg taaaagaatc
    17941 ttcaataata tctgtaatag gagttgttga gataatgcgt actgctgata tagtaaaaaa
    18001 tgcagcattt agagcattag agccattaat agtagcagca gcaatttact ttgttataac
    18061 atttactcta tcaagattag ttggattatt agaaaagaaa ctatctgtgt ctaactagat
    18121 gagagcatgt ttagcattta gggaaaacat agaatagtaa aaaggaaagt gataatatga
    18181 ttacaataaa aaatttaagt aaatcttttg gagatttaaa tgtattaaaa aatatagact
    18241 tagaaatcgc taaaggcgaa ataatggtta tagtaggtcc aagtggttct ggtaaaagta
    18301 cacttcttag atgtatgaat ttactagaaa tacctacagg tggagaaata atatttgaag
    18361 gaaaaaattt ggtcgataaa aaaactaata ttgatgaggt tagacaaaat ataggtatgg
    18421 tgtttcagaa ctttaattta ttcccacata agactatttt agataatata acattagcac
    18481 caattaaatt aaaaaagatg actaaggaag aagcagaaaa aaaagcagaa attttgttga
    18541 gtagagttgg tcttttagat aaaaaagatt catatccatc acaattatct ggaggacaaa
    18601 agcaaagaat agcaatagct agagcacttg ctatggaacc agatatgatg ttgtttgatg
    18661 agccaacatc tgctcttgac ccagaaatgg taaacgaagt tttggatgtt ataaaagaac
    18721 ttgctaaaga aggtatgaca atggcaattg taactcatga gatgggattt gctaaggaag
    18781 ttgcagatag agttattttt atagatggag gaagtatact tgaagataat actccagaag
    18841 aagttttcgg aaatccaaaa catgaaagaa caaaagcgtt tttagcaaaa gtgctataat
    18901 atataaaaag gatgttattg atattagaaa ttcaatacat ccttttttag tgatggttaa
    18961 tattaatttt aaaattaaag ttatattaat attaacttta tttttacgat tttttgcaga
    19021 aaaaaaaaga gctcggggta agctcttttt ttccaccgaa ttaatttccg ttactaatcc
    19081 gatttttaat aaatataaaa tatatcctta tgaaagggta ttgggaatag atatattaac
    19141 taactagggt taccaggggg ataaccttaa tttattataa caaaatgcga cacaaaaatc
    19201 aatatttttt ttgaaataaa ttataaaagt tatttttata acaagtaagc ttataaattt
    19261 aacttttata gtaaaaataa gtatatgtat aatataatat atttatttta tataatatat
    19321 tattttatca tatattgtat tttataatat atttttgtag ggtatagtat aagtatatta
    19381 tattttacta taaaaatttg taataaagtg aaaaaactag cttttcatga aataatattt
    19441 tataatttaa gattgtcttt ttaataaaga ttaacaaaaa aaatactata attagtggta
    19501 taatagttag agttgaagat tttaaataaa ggtaggtgta attaagttgg aaatatactt
    19561 agacaatagc gcaactacaa aaccttatca agaagttata gataaaatgg tttatgcact
    19621 taatacggaa tatggaaatc cttcttctgt ccatagaaaa ggtgtagaag ttgaaaaagc
    19681 tataaaagaa gtaagacaag atatagctaa atctctagga gcgaaagaaa aggagattta
    19741 ttttacatca ggtggtacag agtgtaataa tactataata agaggtatta ctagtttaaa
    19801 taaaaaaaga aaaaatcaca taatatctac taacatagaa catccttctg ttttaaatac
    19861 attaaaagat ttagaagaag atggttttga agtaacttat ttagaggtcg gaaaagatgg
    19921 gaaaataaat atagaggatt taaaaaatgc gataaagtca actacatgct tagtaagcat
    19981 gatgcatgtt aacaatgaaa taggaactat acagccaata ggtgaagttg gaaagtattt
    20041 gaagggttta aaagaaaaaa tttatttcca tgttgatgct attcagtcat atggaaaaat
    20101 taattttaga ctatctaaat acaatataga ttttatgagt gtaagtgcac ataaatttca
    20161 tggtccaaag ggaattggat ttatgtatat aaaagaaaat aatcgcctta agcctatgtt
    20221 aacaggtggt ggtcaggaaa ttggaattag gtcgggaaca gaaaatgtac ctggaatata
    20281 tggtcttgga gaagctgtta gaatactaaa caaagattta gatgttgtta tttcgaaagt
    20341 aaataactta aaaaatatat taaaaaatga aataattgat aatatagaag atataaaaat
    20401 taattctcca gaagatggag tatgtcatat attaaatgta tcatttagag ggacaaaagg
    20461 agaagtttta cttcattact tagagcaaaa gggtatttat gtatcaactg gctctgcatg
    20521 ttcttctaaa aagaaaggta gttacgtatt aaatgctata ggtcttacaa atgaagaaat
    20581 aaatggaaca ataagattta gtttatctga tatgaataca gaagaagaaa tgttagaagc
    20641 tgtgaaagtt ttgaaagaat caatttgtga cttaaggtca ataatgaaaa gaaaataaag
    20701 aatactgtat agatagtgat aataaattag tttaagaggt gaaatggtgt ataatatatt
    20761 aatagtaaag tatggagaaa ttggagtaaa aggaaagaat agatatatat ttgagaatag
    20821 gcttataaga aacataagaa atatgttaaa accaattgga agatttaatg tatataaaga
    20881 gtatggaaga atatatgttg atttagaaga ctatgattat gaagaagtaa ttgaagaagt
    20941 tagaaaagta tttgggatag tgggtgtgtg ccctggagta agagctaaaa aagattatga
    21001 tacattaaaa gaaatagctt taaaaatgtt agaagaaaaa atagaggctg gatataaaac
    21061 ttttaaggta gagtcaagaa gaggagataa gtctttcggg ttgacttctc aagaaatgag
    21121 tatggatata ggtggatact tattatcaaa ggtaggagat aggattaatg ttgatgtaag
    21181 aaatccagaa gttaaaataa agtgcgaata cagagaattt catactatgg tctatagtga
    21241 tactatacct ggatatggtg gattgccact tggaacgaat ggtagagcta tgtctctttt
    21301 atcaggaggt atagatagcc cagtagctac atggatggtt gcaaaaagag gtatggaagt
    21361 agaggcagtt cactttcata gttatccatt tacaagtgaa aggtcacagg aaaaagttaa
    21421 ggatttagct aaaatactgg caaaatactg tggtagagta aggcttcata aagttaatat
    21481 cttagaaata caaaaagcaa ttggcgagaa ttgtaatgaa gaagaagcta ctattctttc
    21541 aagaagattt atgatgagga tagcacagag actttctgaa aaaagacatt gtgatgcttt
    21601 aattacaggt gaaagtattg ggcaggttgc atctcaaact atacaaggac ttacttgtac
    21661 taatgccgtt gtagacttac cagtgtttag accacttata gcaatggata aatcagatat
    21721 agttgatata gccaaaaaaa tagggacttt tgaaacatct atattgcctg aagaagattg
    21781 ttgtagtgta ttttctccta gaaagcccgt cactaaacct agattagaga aaatagaaaa
    21841 atctgaaaca gcacttgaca ttgaaaaatt agtacaggat gctattgaca aaatagaggt
    21901 agaggatatt gagttttagc atacaagtta tttaattact aaattgaata ttgagtatat
    21961 tagtagttct gtaagtatat tagtagttct gttgaaagga tttatgtaga caatttatta
    22021 atatgtagta tttaaaaata agtactataa ttttatttaa tgggaatgat tattatgaat
    22081 aaaaagggtt ttacactaat tgaattgtta gtagttatat ctataatagg cattttagtt
    22141 atagtagctg ttccagcatt gtttagaaat atagaaaaaa gtaaagcagt tacatgtctt
    22201 tctaatagag aaaatataaa gactcaaatt gttattgcaa tggctgagga atcaagtaaa
    22261 ggcaagaatg aagtcatgaa agaggtatta gaaaacaaag atggtaagta ctttgaaaca
    22321 gaaccaaagt gtaagtcagg tggaatatat tcagcaacgt ttgatgatgg ttatgatgga
    22381 ataactggaa tagaaagcat tgcaaaagtg tatgttactt gtacaaaaca tccagatggt
    22441 gttgaaatgg ctagggatat acatcaaagt atgaaagatt tgattgcatc attttcacaa
    22501 gacccttcta taataccagg agcctcaaag ggtaatgatg attttagaaa atatttatta
    22561 gacaataaat ataaaaatgg gtggcctaca attccagatg aatttaaggc aaaatatgga
    22621 ttaagtaagg atacactata tatacaacca tatgcatata gtcctactaa atctgatgct
    22681 actgtagttg tatttgcaaa taataagact gggggtaatt ggtatacttc cctagtttac
    22741 gattatgatg aaggtagatg gtataaaggt aaaaatggta tttctgttgc aggtaggtca
    22801 tgggatgttg acacagatag tgttaagtct gtaaaaacag agattcattc taaagaggga
    22861 tggggtcctt taaattaata tatcatgttt tattaatctg gtattgatta aactatagaa
    22921 aagagcagtt tctatagatg acgaatacca gattttcatt tatatatata attgagatat
    22981 attaaaagtg tttatatttt taagtcttca catacaagct aaaaataatt ataataatat
    23041 tgtaaagaaa tttaattata agattaattt tctgaagatt tagtatttgt actaagaaat
    23101 acataaaaag gggaatagat atgaataata attttcaaga caaccaaaca ataaatttaa
    23161 tacaaagtcg tagaagtata cgaaaattta cgacagaaca aattagtgat gagcaagtaa
    23221 acacattatt acactgtgca tttgcagcac cttcaggatg taataaacag ccttggcaca
    23281 taactgttgt gcaagaccag aaacttttaa aagagattag tgatgatact ttatctagga
    23341 ttcatgaggt gagtaatgtt gagataaata aaaactttaa gttattttat ggtgctccaa
    23401 ccgttttatt tatttcttat gatgaaagta gttcatgggc tccatatgat ataggaatac
    23461 ttacagggaa tataacaaca gctgcacaag cactagggct tggaagttgt ataataggaa
    23521 tggttagagg tttatttaca cctgttgaac aaggggatat tgaaggtctt gtgagtgtat
    23581 tggataaaga agatgtaaaa gaatcagagt caataaagat gaagtttgat acaaataaaa
    23641 aatacagaga actacttgat ataccagaag ggtattctgt accatttggt attgctgtag
    23701 gtattccaga tggaaattta ccaaatgcta gagaggtagt ttataaagtt tccagagttt
    23761 aggataaagt attgcaagaa ccaatcagtt acttgaagaa ttaaaccagt aatatacaaa
    23821 taaattgatg gaagaggaag ctatttaaaa agtagtttag atttttcgtt gtaaaataat
    23881 taaataaaga ataaggtgat aagtatttta tttattatct tattcttttt tatttttgtt
    23941 ttgtagaatg ataaaaataa ggattaagtg taataaaata gaaaacaagt atgttaattt
    24001 taagtatatg ctttaaaatt tatccaagtt aaaaatttaa gatatctttt cagtataatg
    24061 gaaaaaatgt caaaatattg aaatagggaa aaaatagtga tataataaga aaaaatagag
    24121 aaaaa
//
LOCUS       DAMJWS010000068        24021 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMJWS010000068 DAMJWS010000000
VERSION     DAMJWS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.068"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     regulatory      149..250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            323..1666
                     /locus_tag="PC399_002665"
     CDS             323..1666
                     /locus_tag="PC399_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2593517.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            1845..3209
                     /locus_tag="PC399_002666"
     CDS             1845..3209
                     /locus_tag="PC399_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2593518.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(3387..5252)
                     /locus_tag="PC399_002667"
     CDS             complement(3387..5252)
                     /locus_tag="PC399_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2593519.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(5488..6705)
                     /locus_tag="PC399_002668"
     CDS             complement(5488..6705)
                     /locus_tag="PC399_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2593520.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(6905..7768)
                     /gene="aroE"
                     /locus_tag="PC399_002669"
     CDS             complement(6905..7768)
                     /gene="aroE"
                     /locus_tag="PC399_002669"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2593521.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(7850..9772)
                     /locus_tag="PC399_002670"
     CDS             complement(7850..9772)
                     /locus_tag="PC399_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2593522.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(9836..10684)
                     /locus_tag="PC399_002671"
     CDS             complement(9836..10684)
                     /locus_tag="PC399_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF2593523.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            11097..11978
                     /locus_tag="PC399_002672"
     CDS             11097..11978
                     /locus_tag="PC399_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2593524.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(12186..13049)
                     /locus_tag="PC399_002673"
     CDS             complement(12186..13049)
                     /locus_tag="PC399_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF2593525.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(13204..15138)
                     /locus_tag="PC399_002674"
     CDS             complement(13204..15138)
                     /locus_tag="PC399_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF2593526.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(15250..16263)
                     /gene="galE"
                     /locus_tag="PC399_002675"
     CDS             complement(15250..16263)
                     /gene="galE"
                     /locus_tag="PC399_002675"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF2593527.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(16287..17156)
                     /gene="galU"
                     /locus_tag="PC399_002676"
     CDS             complement(16287..17156)
                     /gene="galU"
                     /locus_tag="PC399_002676"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2593528.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(17354..17674)
                     /locus_tag="PC399_002677"
     CDS             complement(17354..17674)
                     /locus_tag="PC399_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593529.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(17664..19655)
                     /locus_tag="PC399_002678"
     CDS             complement(17664..19655)
                     /locus_tag="PC399_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593530.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(19778..22321)
                     /locus_tag="PC399_002679"
     CDS             complement(19778..22321)
                     /locus_tag="PC399_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF2593531.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(22599..22933)
                     /gene="rnpB"
                     /locus_tag="PC399_002680"
     ncRNA           complement(22599..22933)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC399_002680"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23065..23742
                     /gene="srtB"
                     /locus_tag="PC399_002681"
     CDS             23065..23742
                     /gene="srtB"
                     /locus_tag="PC399_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF2593532.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(23902..23976)
                     /locus_tag="PC399_002682"
     tRNA            complement(23902..23976)
                     /locus_tag="PC399_002682"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23942..23944),aa:Thr,seq:tgt)
BASE COUNT         7669 a         4153 c         2709 g         9490 t
ORIGIN      
        1 taattgacat tatattcata catattcaat tattggaaaa agtaattttt ttcaataaat
       61 aaccttaatt ttttataaaa tcgacaaaaa aacgaataat tttattatat ttattgtaaa
      121 taatacaata ttacgatata atacaacaga aaaacaaata gaaaatgttc atataatatt
      181 agtaatacgg tctaatagtt tctacaaact caccttaaat gggttttcta tgaatagaat
      241 gtcaactctg aatatatgtt ctattctatc tatatgaata tatatttttt tgtttatatc
      301 ttaaagtttt ttggaggtaa aaatggtacc aaacactaac aatgtacaac caggtggaaa
      361 taattcatca ttaagctttt tagacaaaat gtttaaattt agcgaacgtg gctctagcct
      421 taaaacagag gtaataggtg gagttactac atttttaaca atggcataca tagttttcgt
      481 aaatccagca atattatctg aagctggaat ggataaagcc gcccttataa cagttaccat
      541 actagctaca gccattggta cacttatatt tgcgatttta ggaaatgcac catttgctct
      601 agctcctggt atgggattaa atgcattctt cacatactct ttggttatag gtgcaggaat
      661 cccatggcaa acaggtcttg gggtagtttt tctatctgga tgtgtcttct ttacactcgc
      721 agtaacaggt cttcgaaaga caattgcaga cgcaattcct agagaattgg cagtagcatc
      781 ttcagcaggt ataggtcttt tccttgcttt tgttggttta aaaaatatgg gtgttataat
      841 agctaatcca gatactatag tttcacttgc agattttaca ccaacagttt taatagctct
      901 ttttgctctt atcttaatgg caatactaga atataaaaaa gttaaaggtg gaatattgat
      961 aagtatagta gttgcaacaa taatatctgt tatatttgga tttgtggagt tgccaacagc
     1021 aataatctcg acacctccta gtatagcacc agttgctttt aaattagata ttgtaggtgc
     1081 attgaaattc tcattaattg gacctatatt ctctttcatg tttatagaca tgtttgattc
     1141 attagcattt cttattactt gttgtaaaca aatgggtctt gaagatgaga atggtgaaat
     1201 aaaagattta ggaagaatgt tatatgcaga tgtttcttct actttaatag gctcattctt
     1261 aggaactagt actgttacaa catttggaga atctgcttct ggtattgcag caggtgcaag
     1321 aacaggactt gcttcaatag taacagcagc attattctta gctacactta tatttacacc
     1381 attacttggt atagttccta cttacgctgc agctcctgca cttgtaatgg ttggtgtatt
     1441 catgtttgaa agcataaaac agattgattt tgctgatagc aaaatagctg tacctgcttt
     1501 tgttacagtt ttattgatgc ctttaactta tagtataagt attggtttat gctttggatt
     1561 tatttcatat ataataatgc atgtagtagc taaagaaact gataaaatca gtattacact
     1621 ttggattata ggtttacttg cagttgtaaa cttagttcta agttaatatt tttaaatagt
     1681 tctaggtcaa tattttaaat tatatagaat aaaatcttat ttattttaaa tttataaata
     1741 gcaaaatcac tttatatgta aattgatttt gctattaact ttgtatagaa aatttattat
     1801 cataatatat agatataaaa atatatagaa aaggagtggt gtttatgtca caattactta
     1861 taaaaaatgc atatctaatt actatgaatg cagatagaga ggtatatgaa aatggaagca
     1921 tacttattga aaataatatt ataaaagagg ttggtaagtt tgatattaat attgtagata
     1981 aagatgcaga gatatatgat gctaaaggaa aaatactaat gccagggcta gtcaatactc
     2041 atgttcactt atctcaacaa ttaggacgtg gtcttgcaga tgatgttgta cttttaactt
     2101 ggcttagaga aagaatttgg ccatacgaaa gtagcttcaa ttatgaagat tccttgattt
     2161 catctactgc atgttgtgtt gaattgataa aatctggtgt cactacattt ttagaatctg
     2221 gtggtcaata tgtagatgct atggttgaag ctgttgataa aactggactt agagcctgtt
     2281 tagctaaatc agttatggat acaggagatg gtcttcctga ggcatggcaa aaaactactg
     2341 atgaggaaat caacactcaa ttagacttat tccataagtt taataataca tctaatgaga
     2401 gaattaaaat atggtttgca ttaagaacta tttttaatac aactgaagaa ttacttgtta
     2461 gaactaaaga actagctgat aaatacaata caggtataca tatgcatatt gctgaaatag
     2521 ctgatgaaat ttcatttgta aagcaaaaca atggagttgg aacagttgaa taccttgata
     2581 aactaggtgt attaggacca aatcttttag ctgctcatac agtttggctt actcataagg
     2641 aaattgattt atttagatta catgatgtaa aagtttctca tgacccaggt tcagctatga
     2701 aagtggttct agggtttgca tcaattcctg aaatgttaga aaaaggaata cctgtttcaa
     2761 ttggtactga tggagctcct tcaaataata gaatggacct tatgagggac atgtatctta
     2821 catcactaat acataaagga agaactttaa acccaactgt cgtaccagct gaagaagtct
     2881 tagaaatggc tactataaat ggagcaaaat gtgctttact agaaaaagaa attggtagtc
     2941 ttgaagtagg aaaaaaagct gatatgatta ttctaaatcc agatacaatt cattgtttac
     3001 caatgcataa tccaattgga aatatagttt attctatgac tagtgaaaat gtagactcta
     3061 ctatttgtga tggtaaatgg ttgatgaagg aaagaaaagt attagttgta gatgaatctg
     3121 aattattaaa taaagttaaa aaacaagctt ctaaaataaa agataaagca gaagttaaaa
     3181 ttccatctaa atttaaaatt ataaaataac ataaatttca attgttttgt aaatatgaat
     3241 atataaatat atttatatac atcatatata caaaacaatt gtttttttat tttatattca
     3301 ttagcattgc tcataaaata caccataatt ccagaaaaaa taaccaatca gttaggatta
     3361 catcatctaa gcaatatata aacgtcttag tttataagtt ttattctact cttatctaaa
     3421 accatcttta aagagttttc aactgtttca attacatctt tataactcaa gtacacattt
     3481 gcgtagatac aatcttcata taaattatat tcagttactc tacaatacag cagttttcct
     3541 ccatctaata aattgctcaa aatatagttt tcttttcttg ggacatagcc tatcacatat
     3601 ccagtttcac caactatttt aatagctttt tcatcatata gattatcttt ctctctagtt
     3661 aaatagtata tcttactttt ctccaattct ttttcaagat attctctatt tctaaacctt
     3721 gaaccagcaa cagctattga agtcaaatag atatccttac catgttcatc tacttttatt
     3781 ttttcaatat ttacaaattc tttttttaaa ttattacttt catataatac tcttcttaat
     3841 tcatccttca atataacatt tttttctttt tcatacgctt ctttaacaat ttttaattct
     3901 tcaagagata gtttctctct gtatcttttt aagatattta tggattcttt tcttacttca
     3961 tttatatttc caaatattcc ttttaaagat atattctttc cttcaggata tagtgattta
     4021 cttcctttaa gcacaataaa aaataacata tcatcataat attcttgagt caatttatct
     4081 ttttctatat ctttcatttt tccaattaaa tcatctattt taaacgtctc ttcaaaaaaa
     4141 tttaataatt ttaatctaga acttgtcgtt ccctttttag atatatgcca atatacctca
     4201 aaatctctaa tatctctatt tagatatggc aacaagtcat caaaagataa gtttacatca
     4261 taaatttctg acatttttat aatatcttct gtttttccac tagcatctct taatgcttca
     4321 aaatatatat ttttccattt ttcactgagc aagataccat ttatttcttt ttcaaattca
     4381 atcttattgc actctatatt ttcatctttt aagacgttta tagctattag gaaaattgaa
     4441 tataaacttt caaagttagt cccataattt actacagtag gtaaaaatcg attcactaat
     4501 tctaatttat tacctatata tttaaattct acacttagca agtaattgca tataagacgt
     4561 gctaaattat ttatcttttc tttatctaaa tctcgttttt caagatattc attcaaatct
     4621 acaattgaaa taatatagtt catcaataat cgttcgtact ttgtatcctt ataaccatct
     4681 tcaattagat aggaatttat tttagaatct aaattttcca tattaacaat agcaaatact
     4741 tttattgaac ctgtagcctt tttagataaa ttaaataaat atgtattgta aaattcaagt
     4801 tttttaatag tattactcag ataaaatacg tattctccag atttagaaaa tgtatctaca
     4861 acttctctta agttttctac atttagatat ttctcactta aaactaaccc tagttttaca
     4921 tactcaactt ttttactctt atatattaat tctttagtta atttatataa ccatttctca
     4981 tcaataatca tttcttttac caaagtattt atctttttca taaaaatttc gaaataacaa
     5041 attaaagact tagaattact accctccaat aaataaattt cattagattc acttttatac
     5101 atatttaaaa tttcatctac tagattattt gcaagctcta cttgttctct taacggtata
     5161 ctttcatata tacataggta attcatttca aatgattctt tgtatccatc ataaccatta
     5221 aattcctgca atcgcttaaa aacattctcc accaaattat cctcctttac atgatgtaat
     5281 ttatattgat tatactactt atttcatcaa gtttacattt atacgcaaaa aaatattact
     5341 agaaattgaa attctagtaa tatccttatt attaattatg taaataccct atcttataac
     5401 tgtaaataat atccttatta attttaatta cttttaataa aaatctatcc tagatttaga
     5461 cttctctatg catatattat actaatatta gacatcataa tcatacactt tttctttttc
     5521 atatctcatg tttacaaaca atgcaagtaa aattccaaca aatgttattg ccacattaaa
     5581 caatacaaca tattttggtc cattaactcc ccctgatttt gtaataatac ctgctatatt
     5641 taaaataaca tagtttgcta tactagatgc aatcataact atagatgtta tttttccttt
     5701 attagttgga aacatttcat ttgcagttga cgttgttaac tgaagaacac ctcctgctgc
     5761 tgaataacct attacaaatc cccctaacat acaaatagtt ggagtttgaa caaaatatac
     5821 aactaatagc ataagtaaag ctattatagg atatataact aatattctta ttggtttaat
     5881 atatttttta ataagtaaag aagttattaa tactgcacat attactccca ttgagtaaaa
     5941 ggattgaatt tttgtagggt cagccatccc atacaattta cccaattcct gattacaatt
     6001 taaccataat acaaatgtag atgtacatgt aaatccaata caaacaagag ctatacttgt
     6061 tggtgtaaat ttcatcttct ctgatttaac tgttttgcct ttgttatcaa taacattatt
     6121 agctggtgga aatggtaaca cagctatcaa aaaggcatct actacaataa gaatcgctgt
     6181 tactataaaa ataactttaa atgatattga atttgctgca acaaatccaa ttataaaagg
     6241 aagcaaaaat tgtgctaaag ctattgtaaa tttagtaaac atatttgcta ttgttcctaa
     6301 actagcaaat atctccatac aagaaggagt tacacaggta tctaagaatg aatttgctgc
     6361 ccctccaatt attgcaaata catatgctat ataaaaattt ggtgaaaata caattccaac
     6421 aaagaaaatt gcatgtagaa agtttcctat aagaccactt acttttcttc catacttatc
     6481 tgaaataggt cctgcaaatg gataagatat aagtctccct aatcctagtg cagcaataac
     6541 agctaaaaca atacttacat ccaatgttcc atctgataat gttttagccc cccacattga
     6601 tgaaaattct ggtttgtatt gtcctaaaat tgaaattcca ataccaagta aaaaatatga
     6661 aaaatacaac gctatggcag taggataata ttttttattg ttcataattt ttctcccatt
     6721 cttgttaatt tattaacatt tttgtgtaca ataaactcat ccccataatg gatttaaagt
     6781 atagtcagtt aaaacttcaa atttaactgg actatgaggc aaaacccact atgacaattt
     6841 gtaatttatt attaaaaata ttgaaatacg tatgtttttt cattgtttaa gatattactc
     6901 ttatctattt aaagcataat tcattaactt cttctacagg catttctaat ccagtatata
     6961 gattaaaagc ttctgctcct tggtataata acattcctaa tccaccaata gccttacatc
     7021 cattagcttc agcatcttca atcatttttg ttttcttagg attataaacg acatctgtta
     7081 ctactaaatc ttttcttaaa acactagtat cttttatatt tgtttcattg tcataaggct
     7141 tcataccaat aagagttgca tttgtcaata tatcacttga ttgtatctcc tcataaagtt
     7201 tgtttgtatc ttctaaatca tatagattaa caacacattc tggtacatct ttttttatat
     7261 tctcaactgt ttgctctgct cttttataaa aatcatcctt aggattaaag atagatattt
     7321 ctcttgctcc atctaaagca cattgtactt gtatagcagt tgctgcacca ccagcaccca
     7381 taattgtaat ctttttacct ttaacttcta ccccattttc ttttaaatta cgaacatagc
     7441 ctactccatc tgttatatga ccaactaact ttccattgtc atttacaatt gtattacatg
     7501 ctccaattat acgagctgct ggagaaagtt catccatata ttctgtaaca gcagatttac
     7561 atggcatagt aacattggct ccttttatat taaaagtttt tattgctaaa agtgcctctt
     7621 ttaccttatc tactgtgata tcaaaggcta aatatctata atctaaatct aatttcttaa
     7681 aactataatt atacatcact ggtgatttag agtgaccaac cggtgtacct attaatgcaa
     7741 ataatcctgt tctacctgaa atttccataa ctataatctc ctctcaatat tatcattttc
     7801 tttgcaaata acctattcag ttatttacaa agaaaaattt atttattatt tatatattta
     7861 atgctacttc aaaagcttct tttgttgcat ctaatgccct acgagctttt actgcatcac
     7921 caacaatata agtttctttt acaacttctt ttataacttc acttaatggg tcgtaattac
     7981 gagaacccat agcaagtact actgaatcaa aaccttttaa atcatgctct gtgccatctg
     8041 taagtaaata gtcgacacca tcatcataaa atttagaaac ttttgcacca gtaatggtat
     8101 gtatattata attttcaaaa tcattcataa ggaatttacg gtgttctgaa attacatctg
     8161 caccaacttc ttctctaagc tcaataacag taacctcatg ttcttgttct ccaaggaagg
     8221 ctgccacttc acatccaacc attccacctc caacaacaag tacttttttc ccacaagatt
     8281 ttttaccatc aagaaggtct acagcatgaa ttaatcctga atcattgatt cctggtatag
     8341 gaagtacaag tggagttgct ccagtagcta taataactac atcaggtgat ttctccttta
     8401 taagttctac tgtagcttct gtattagtac atatttttac accatattga ttacattttg
     8461 aaatataact tctaactaag ttagtaatat ctcctttccc tggaggatat gcagctaaac
     8521 gcatttgccc accaagaact tcagattttt caactaaagt tacattatgt cctcttttag
     8581 cacataccca tgctgcaagc atacctccaa cacctccacc aacaacaaga acttctttgc
     8641 taatttcagc tggcttaagc tctgcttcat gtcctaatat aggatttgca agacaggcta
     8701 taggtttgcc ttggaacata tttggtacac atccttgtaa gcatgcaata catggtatca
     8761 tttcgtctag ttttccattt ttagctttat taggcatttc tgggtcagcc aagctttgtc
     8821 taccgaatgc tactaaatca catcttcctt gtctgaccat taactcagca aactgtggct
     8881 ctgtataacg tccaactgtt attactggta tactaacagc tttttttatt tcagtaacta
     8941 attctgcact gaatccagca tgtaaagtcg taggagccca catatattca tcatgtatat
     9001 gtatactacg tgaaacatga agtccatcta ttccacaatc ttctagatat gctgctatta
     9061 ctgaactatc gtgtacatca attcctcctg gaacatcatc agtgctgttt atacgacata
     9121 atattgctag agaatttcct acttttttac gtatattttc aatgataaga cgaggtaatc
     9181 tcattctatt ttcaaaacat ccaccaaact catctacacg cttatttgtt ctttgtgata
     9241 taaaactact aaccagatat ccatgagcgc agtgaacttc aacagcatct gcaccagctt
     9301 tttgtgcacg taatgcagca tctccataag attctataag ttcatatatt tcttctgttg
     9361 ttattgctag tggtgtatta cgaccatttg atgcaggtat tgcagaagca gcttttaatg
     9421 gatgtcctgc tactttttca tttccttcag aaccagcatg ttgtaattgt atagatacct
     9481 tcgaacctgc atcatgacaa gcatctatta catttttaaa actatctatt gtgctatcat
     9541 catataaaca aggcttacga ggacctcctt ttgctttttt gtctacaaca ctagattcta
     9601 tagttattaa tccaaaacca cctaaagcac gctcaagata ataagcctta gaagtttcac
     9661 ttaaagtacc atctgtgttt gcaaaattgt ttcccatagg gggaactaca aaacgatttg
     9721 gaactgtcat tgaaccaatt cttataggtg taaacataga actaatcttc ataaggtttt
     9781 ccttcctttc aagttttata ttataatact ctgtatattt ttatattata taagtttatt
     9841 ttggagatac ttcagaccat gctttatcca aaactttttt agttgcatta aatacagttt
     9901 cagcagcagt ggataaccct ttagatatta gagaatcaga tattacttct gcccctatga
     9961 ctcttggtga tatattgtgt tcttttatta ttttcacaaa ttcctctgtg tttccatatc
    10021 cttcacctgg agccaaacgg tcatgtagtg attcatccct aagtttttca taaggagttt
    10081 ctaaaacatc acacacttga attgatacta tcttatctgc tggaacaggt gcaaggtcct
    10141 ctgctgtctg ctttgctcta gcccagtgcc aagtatctaa tataagcatt gcattttcac
    10201 gattacatcc tttcacaaca cgccatgcag tagccaaatc tggtacacca ctatatggca
    10261 taaactcaag acctataata agttcacctg ctctgtcaca taattctccc aatgcagtta
    10321 taatttgttc ttctggtatc atctctaata atccacaatt tatatgttta acattaaata
    10381 agtgagccat atgataaaca ttttgctctt tttctctttg agcttccgtt ctatcttctt
    10441 ctcttcccca ttgagttata tattctactt ctgtaacttt catatcatat tcattaagaa
    10501 ttgcaatcat ttcttcatct gtaacacctg cattttttgc atcaatataa ttttcagcac
    10561 gcaatccaat accatcaaac ccagcttctt ttgcaatacg aactctatca cggaatgaaa
    10621 cttctgttcc caatgtataa gaactaattg taattggaat attaagattt ttttgattat
    10681 tcatagctta aatttgcccc ctttattttc tttataatta atagtatttt tgattttata
    10741 tttttattta tcctactagt agtattattt taccttaaaa ataaaagtat tttttgattt
    10801 ctatttatct agattttata aatttattgt atcacgagac tttactaata acaaattgat
    10861 aatttcctat taattcatag attttgttta tagattttat ttataagata attcttgtat
    10921 ttttctatta ggtcgtataa atatatttat cttataattc agacaaaaaa cgataaattt
    10981 ttttaaaagc ttttattctt actctataat catatataat aaaaatgtaa aaatatctta
    11041 aaggttaact tctacaaata tataaataaa aatacctatg aaaggatgaa caaaaattga
    11101 atttatatca cttacgctat tttgttacat tggcacattt agaacactat acaaaagcgg
    11161 cagaaaattt atctataaca caaccaagct taagtcatgc tatttcttta ctagaaaatg
    11221 aacttggggt ggctctattt gaaaaagaag gtagaaatat agtccttact aagtatggaa
    11281 aaatattttt aaaagatgtg gaaaaatcat tagaaatttt agattcaagt gtaaaatcat
    11341 taaaaataac tggtactggt gaaggacaaa tagacttagc ttttcttaga acacttggta
    11401 ctgattttat cccagacata gtgcacaaat ttttaaaatc taatcctgct aaatcaatag
    11461 attttaaatt tcatactggt gtaactactg acattattca aggtcttaaa gaaagaaaat
    11521 atgatattgc attttgttcg aaacttgaaa aagaaaaggg aattgaattt atacctgttg
    11581 ccaaacaaga tttagtacta attgtaccat atagtcatcc actagctgca aaggatacta
    11641 tagatttaaa agaaaccata ccttatcctc aaatagtatt taatcagaga agtggtctta
    11701 gatatataat tgatgatatg tttaaaaaaa taaatcaaca gcctaatata gtctatgaag
    11761 tagaggaaga ccaagtaatt gctggattag ttgcaaaaaa tttcggaatt gctgtagtcc
    11821 caaatatgaa tatgcttagc tttacaaaag taaaagtaat tcagattatt cacccaagtt
    11881 gggaaagaaa cttttactta gcatttataa aagatagata tttgcctcct gcaattaaaa
    11941 actttaaaaa ctttgtcatt aaaaacgctc aattataatt tattaataaa ggggttattt
    12001 ttaaatatcc tcttttattc tttttataaa ataaaaaaat ctatactttt aaaagtatag
    12061 attcctaact gacagtcggc atccgagtct cagtaattat cgttatacaa cagcaactgg
    12121 attcagttcc tgaataacta ggttacttac attatatcag attttttaaa atttgtcaat
    12181 tgtatctaac gcattttttc aagccatggt ccaacaggct ctacaatagg aacttcttta
    12241 aatccccaat ttcttatatc attcacactc gttggagtat atattttgtt tctgacagat
    12301 ttgaatattt tgtttttttc tccatattgc atacactttg gattagcttc tattaaccct
    12361 cttcttgctc tgctaagagg attacataaa atatctgcta tttctaaacc ttctctatag
    12421 tcagcatctt cttttttggc aattataaaa cccttaacct tatctcttaa ttcttcatct
    12481 atatctaagt atattgttcc attattaaat acatcaaaaa acgccttttg tacttttaag
    12541 tttgagacat catcccttga ctctataact attcgtgcag attctacagt ttcattattt
    12601 acaaatgaat aaaaattctg aagaacatgt ataaaagcca caacatacgg ttcctttgaa
    12661 ggtgcataat atttctttaa tctagcttta tctactgtta ctgatattat agtacactct
    12721 aatctagata gaaataaagg caactcatca tagaataact taatctgtga atcatcaaac
    12781 ttctcaaatg ctcttgattt tcttgatata tctgtcaaat gtattcctat gttactggtt
    12841 ccaaaacaat actctttaaa tgcatccatt tctttcctca aagaacatac ataatcgcct
    12901 tgacttactg catatttata ttcaaaaatt actcctgtca atgtaaatgg gtcttcatga
    12961 tttatatcag ctcttccact ttcatcaata aacattatat attttttctt tataatctta
    13021 ctcaccctct taacatatga tgatagcatc tatattcatt ttatatgagt tttaaaaata
    13081 ttccaagatt tttatataac ttaaacttaa ttttatacta aaaaattgag atgtaaaaca
    13141 atcaagcttt tattgattga atttcacatc tcaattataa ttatttttca atatatattt
    13201 ttactatttt gcaagaatat ccattaattt atccattact atataagata ctccgcctcc
    13261 tatttgataa ggagtttcaa cttttttatt tgcaaatact ctttgttgct ctgatgataa
    13321 tcctgaatta tccattaaaa ctattggtga atgtaatttt acagctaatg gtccagctga
    13381 taaagcatct actaaagcat ctgatttact aactaataca gcctgtaaat cagtatctcc
    13441 atagaatttt tctatcactt tggcattagt atctactcta ctatctccat atattctatt
    13501 tcctgaaata tcttcagtag ttatatcatt catttttgct attactgaat cgttaattgc
    13561 acttggtcca cctataaagt atgcattttc taattttctt tctgttagcc atttataaga
    13621 ttcttctgta attccatctt tattgactaa tattataggt tgtttttctt ccccagcttt
    13681 agaagctaca gataaagcat ctgcctcccc atttccagaa accatatatg cagtttttat
    13741 ttcttctcga tttccaagtt cttcagctat tcttgtagca gtttgatatc tatcttgacc
    13801 tgctattctc tttgtttctg tgttgaaagt agtttttatt tcttgctcta atttagaact
    13861 tattgcattt tctccaccta tcaagattac tgttttaggt gctaataact tcatttcttc
    13921 aacagtttca gttggtaaat tagctgtatt agtaagtagt attgttgagt catagctact
    13981 tgctaaagga gttgctgtta taccatcagc tattgcatta ccatttacta atattacagt
    14041 atctgaacta tcagcattcc agtttccaac tgaacttatc tttttagctg tatcaaatct
    14101 atttgcacct ataattgtat cccatgtagg atttatagtt ccatcttcat taaaagtata
    14161 atctttccca tctatttttt gagaaccagt tagcatcttt ccatctttgt ctaaatagta
    14221 agacttacca tctatttgtt gccatcctgt ctgcattatt cctgttttgt caaagtaata
    14281 cttattacca tctatttctt gccatcctgt tactgctaaa ccatcagatc ctagatagta
    14341 tttctttcca ttcttttctt gccatcctgt ttgcattact ccatctgagt taaagtaata
    14401 agtttttccg tctattactt tccatccaga tactcttgct ccatcagttc ctaaataata
    14461 ttttttgtta ttaattgttt tccatcctgt ttgcattact cctgaagcat caaagtaata
    14521 catttttcca tttactttta gccaactatt cttttggtat gttccatcag attttacata
    14581 atacttctta ccattttctg ttttccatcc tgtttgattt agatttatct tatattttgc
    14641 cgctatcttt acatagctgt cgttactata atctataatt acatctgaac ctacaggaac
    14701 ttggtcaaat aaccatctaa catctttatt gtgcattctt atacatccac cagaaatatg
    14761 ctttcctata gaactctcat tattatttcc atgtattcca tatgtatctc cataagttcc
    14821 ttttaatgct aatcccatcc atctatctcc taatggattt cttggacttc ctccaggtat
    14881 attaccttta taatatggtc tattttttat cttgttaact acttttgttt tccctgttgg
    14941 tgtttctgac ccctttttac ctgttgcaac tctaaattct tttacaagtt tattatttac
    15001 aaaatatcct agagtatttt ttcttgaatt tactataatc acatgtttac tggctgcgct
    15061 tgattctggt acgccacatg caaatactaa aactaaagtc agtgctagta ctaaatactt
    15121 ttttagactt tttttcacta taacttcccc ctcttttaga aacattacta catgtgtcta
    15181 attataacat aaaattacca ataccttata tttacaaatt tgttacattt atacatataa
    15241 gtatgtattc tactcttcat acccattagg attcatactt tgccatctcc atgaatcttc
    15301 acacatctct tcaagttcat acttagcttt ccatccaagc tctttttctg cctttgaaga
    15361 atctgcataa cacatggcta catctccagc ccttcttcct acaatcttat atggtatttc
    15421 ttttccactt gctttagaaa atgcttttac taaatcaaga acactatacc cctttccagt
    15481 tcctaaatta taaacaacaa gtcctggatt ttcttctaat ttttgtaatg cttttacatg
    15541 accagcagct aaatctaata catgtatata gtctctaact ccagttccat catgtgttgg
    15601 atagtcatta ccataaacac tcaattcctt caatttacct actgctattt ttgttatata
    15661 aggcattaga ttgctcggaa ctccatttgg ttcttcacct attctaccac ttttatgagc
    15721 accgactgga ttaaagtatc ttaaaagagc tatatccaat gacttatctg cttttgatat
    15781 atctactaac atctgctcta tcataagttt agttcttcca tatggattgg ttacagaaag
    15841 tggaaaatcc tctaatatag gacaagtatg tgggtctcca tatacagtag ctgaagaact
    15901 aaatacaaac tttttaacac catattctct cattaattca aataaattta aggtacttat
    15961 tagattattg ctgtaatatt caattggttt ttctactgat tcaccaactg cttttaaagc
    16021 tgcaaagtgt ataattgatt caatattatt ttctttaaat acaacatgca tttcatcttt
    16081 atttctgata tcaatattat aaaattttac tggtttttta gataattcct tgattctatc
    16141 aaccactatt gagttgctat tacttaagtt atcaactata acaacttcat aacctgattc
    16201 taaaagttct attgctgtat ggcttcctat atatcctgct cctccagcta ctaaaactgc
    16261 cattgtaatt acctcttttc taatatttat ttttcactta ctttttttct catatagtcc
    16321 attaaatcat cttttaaatt ttctctcttt agagcaaagt ctattgttgc ctctaaaaaa
    16381 ccaattttat ctcccacatc atatcttcta ccttcaaagt tataagcata aatagcctct
    16441 tgttttccaa gagtttgaag tgcatctgtc agttgtatct ctccaccttt accaggtgtc
    16501 tgtttctcta atatgctaaa tattgcagga gttattatat atcttccaag tatagctata
    16561 tcagatggag cttcttcaat tgcaggtttt tcaaccatat ctttaacttt atatactcta
    16621 tcttcgatat gttttacatc caatatacca tatttattag tatcttcttt agctatcttt
    16681 tgaacaccta atacagttgt tttatactca ttataagtat ctattaattg ttttaagcac
    16741 ggaacatctg aatctactat atcatcacct aataatacgg caaaaggttc atcacctata
    16801 aaactttttg cacagtaaat tgcatgtcca agaccttttg gctctttttg tcttatataa
    16861 tgaatattta ccatatttga tatgtctctt accatttcta acatctctgt cttacctttt
    16921 tgctctagtt ctaactctaa ttcaactgat ttatcaaaat gatcttctat agatttttta
    16981 tttctacctg ttattataag aatttcttca attcccgatt ctatagcttc ttctattata
    17041 tactgtaaag ttggtttatc tactatagga agcatttctt tgggctgtga ttttgttgct
    17101 ggtaaaaatc tagttcctag ccctgctgct ggtattattg cttttttaac tgtcatctgc
    17161 attttttcac ccttcttcgt aaattttcta ttgtaccaaa ttaatatacc atatttatac
    17221 tttttcttca aactctatat tatcccaata tttaatgcta taagataata taaggtagat
    17281 gaaatttcat ctacctttag ttttataagt taatattgta ttgtttatat tttatccaaa
    17341 acttatacag gtactaaatt tcatctaaga tgccttttat atcactatct atatcttcat
    17401 ctagataaga atctaaatct tctaagtcaa cttctagttc ttgatatgtc atatcaacac
    17461 tgacatcctc atctatatcc atttctgata ttaattctcc taaaatacta tttccatcag
    17521 aatctaaacc actatctata tcttcatcta tatagttttc atttaaaatt tcatctaaat
    17581 caatctcgta ataaacttca ttattttttt catcccctga gtccaatcct aagaactcat
    17641 agttaaaagt ccaaacattg tatttacttg acatacttta ttcctcctgt gaaaaacata
    17701 ttttcatcta gtttattggt tccataatca acagagttat atattttttc aattatgtct
    17761 tttcttcttt cttttgtact attatttaat gtcaattgtt taaatacata tgtatttgtt
    17821 attctctcga ataatatttc ttttatacta tcatcatcaa tatttctata gtttttccac
    17881 aataactcaa ataacttaaa catttcccta ctagtttcat ctacataata aggatacata
    17941 ttttctacta aatggtttaa tcttgatatg gcttgtttta aaaatacatc aagacttgca
    18001 ctttcacttt cttttaacat tctataattg tatttcttct tatcttctcc tgaaattaca
    18061 aagatgatat tgtctaaact tttttcaact tcatttaaat aactgcttat attatatgat
    18121 aaatcagaca atagattttt aatctgctta aacacttgta catcaagttt agtattatac
    18181 ttttcaaaca aatctatttc ttgattttct aatattcttg gcatatatgc tgacaataaa
    18241 tctaaattgt ttctttgttc ttgtatttgc ttataaagtg aatttataac ttctattctt
    18301 atttcatcat ttaactcttt ttctaaaatc ttatcaaatt cagatttcat atagtaaata
    18361 tttccaaaga aattaaatat tatcatatca ttcatagctt tagaccaatt cttatatatc
    18421 acttcaatag aattttcttt aagtagaggt tgctttgcat atataaatac atcttgcata
    18481 agtttttgaa ccatactttt acttaagttt aaaagtactt tttgattatt aaattttatt
    18541 tccgtttcta caaaattaga attttcgttt tcaagttctt tattaattac aatgtctttt
    18601 atatcactat tttttataga tatgatttct ttttgaaaag actttagaat agtcaagcta
    18661 tcgaaatctt cttgaataaa aaatggagca tgttttaatg tatatcccac tatttcattt
    18721 tctatatctt ttaggtcatc tatttgcata atttctctat ttttatcatt tatatgtagg
    18781 ttaaatacat ttttattatc atataatatt atatcttctc ctaaatttaa cttagaacaa
    18841 ttatctttcg ctgactttac aatagtcttc ttagatttct tatttataaa aattaattgg
    18901 tcttgatatt ttactaaaag catgtttgtg tttttataat caaatccatc tatatttcct
    18961 gatatcttgc agaatactat ttcacaatct ttaggaagat ttcctatttt ttggttatct
    19021 ttaactctct catttaaatc agatgggact acactatcta tattgttatt aaatatagat
    19081 acagacctta ctataatccc ctctctttcc atatgaaagt atcccttcaa tgacactctt
    19141 ttatcttcta aaatgtatct ttctatatca tcaaaattta tttcaaatct atttttccca
    19201 ctgataaata taaacttctc attatcttta ttaattccac ccatataaaa cttattgtca
    19261 tctaaactta atatagtcat attatccttt agatagaata taaattctct ttgacttttg
    19321 cagattaaat catattcatc caaagttatt ttcatagtca aatttgctgt aacattttta
    19381 cttgaatttt cactttttat gtaaaaaggc aagttatttc catagacaat tatttgagaa
    19441 tcttctaaaa ctgcctctaa tttttcttta ttgtctgaaa aactatatat atattttaca
    19501 cctgtatcct cattaacttc atctgatttg ctgattatta tattccctag atttgttata
    19561 tgaagctttt tatattcttt tgagtatcta cttatgccac taagctcaaa cccatctgaa
    19621 agctcatata tgattttttc tcctaatttc aacatacctt caccacctaa acagaattat
    19681 aagtatttta taaaggtgaa taaaatgtct caaaacaaat aatatttttt ttaatacttt
    19741 ttattctgag acatagtttc tcctttttta taatgctcta agcaccttca actatttctt
    19801 cataaagttt atctaaatgt ttaacatatt cacgatatcc agatttgttc tttaactcac
    19861 ttcttaaatc ttcatatttt tgtttaagtt cttcagcact caaagaagga gctgattctt
    19921 ttaattcttc taacttataa tcatagtact tatcatacat agctatatat gtatcaatag
    19981 acttcttagc tagttcatta catttttctt gtgcttctcc atattgagaa taatcaccta
    20041 tttttatttt atagtatcca ttttcttgaa ttattgttga agaaatacct ttagcttcat
    20101 attcttttgc tgtttctttt gctgtttttt caactttaag tgaacctgca taaacagtgt
    20161 aatatacacc tttaccatct ttatatgctt ccatttcttt tgctttcttt tcagcttgtg
    20221 ctttttcttg agcctctttt tcagcttttg ccttttcttc tgcttctttt ttctcttttt
    20281 cagcttgtgc ttttttctct tcttcagtta atttattgtt agcttgttgt tgagttgatg
    20341 actttggaat attctcttta tcatttttct tattgttgcc tactgttata aaatatccta
    20401 caccaacaat aaccactata actactgcta taatagtttc ttttaatcta ctcttcttag
    20461 aaacaacctc ttcatcatct aaagaatctt ctttatctga tacataattt ttcatttctt
    20521 tcttgttttc tctatatttg tccttagaag aatttttaga ttttttatta ctattttctt
    20581 tctttttaat aggtattact actgattttt cattttctaa tttttcttct tttatttctt
    20641 ttgctttttt atcttctaag tttttacttt ctttttcaag cttcgattca ttatttgaag
    20701 atactttttc atctaagtta ttatttattg gtttttctat ttttacacta cgttcttctt
    20761 ctatatgttg acttggttct tcttgtttta tttctttcaa ttcattttgc tctatttctt
    20821 ctgatttcac ttgatgattt tcaactggtt tatctaaaat tatatctgga gaaatatcat
    20881 ttaaattctc ttcttcttgt tcagcttctt cattgtcttt tttaataaat ccaaataatt
    20941 tagccttctt tttactcttt ttattttctg gttcaattat atcttcttta ttgctgtcgt
    21001 actctttatc tgtagtatct ttatcttcta caacgtcacc tatatcttga ttattactat
    21061 ctacttcttc tttagtccct tcttcagcat catcttctat tgaaatacta cttctaaagc
    21121 aaatatcttt atctgcattt tttatcatgt aaatgtattc ttcaatatcg atatctttat
    21181 ttcttctagt tctttttgat gcaatttcat gattttctaa ttcactatag ttatctttta
    21241 ttagattctg tgctttattc catgtatctt cactaatttc tgttttattt ttaattacat
    21301 agtccacaaa ttccttaaaa ttttcaggac caaattctcc aattatagtc agtctcattg
    21361 caccttctat aaggtctcta tttcttctct ttaaaaattc taatacaact gttgcacaag
    21421 caaaatcttt aataatttct ttgccaaaac catatgcttc aacagaatga agttcttcta
    21481 atccacataa cttcttatat tcattgtgta ttgtttgaga tatactttca ttatttaagc
    21541 tatttaactt atctataaac ttcgattcta tatcatttga ttttgattca tgttttactt
    21601 ttgaattttt aatttcattt agtagcatgt cattttctga attatcttta cctaattgca
    21661 aatcctcaat ttcagttgct tttatttcta ctttgttttg ttcgttattc tcttccacat
    21721 tattaggtac cacatcttta ttactagcta atattttgtt tacacagaat ataatttctc
    21781 tttttaattc tcttttcgca tctgcttgat ttttaagaaa taaatctcca cattctttct
    21841 tttctaaact tatttttcta agagatatat taagaaggtc tttatatagg tttaaaacct
    21901 tataatcctt ttctaaacgt cttcttctct ctgatagttc cattttatca taagctaaaa
    21961 aactatttaa atcattggct tttagagagt ctaatccctc tttcatagcc ttatttacat
    22021 actttctaca ttttgctgta gttaactcta aatctttttc taattcatct atttcaggta
    22081 tgtacttatc tactatccat ttgccaataa gtggttctgt atttcttgct aatgtcttat
    22141 aaaacaatat attaaatttt aaaagctctg catattttgc ttcattaaat tctacttttt
    22201 caaatttctt ttccaaaaca ccatctagct tttcatttat tttagactct tgttttaaaa
    22261 aacttgctgt gacatttaaa acagtgtttc ttattatatt attttcactc aaaaccttca
    22321 ctccttaaat caattatttt attgtaatat ttacattgtg caaagttgct aatttgatta
    22381 tgatatctaa ataattattt actaaatttg ctacatctta tatcatatca tattatttac
    22441 atcattttac tttttctttt tatatctatc ttatattata ccatttttaa tatctgtact
    22501 aacttatatt acaatattcc tcaaaatttt aaaagtagat tagacaaatt tacaaataaa
    22561 aaaacatcag tttaaactga tgtttttgac tatcgaaata attaagcaag tctataagcg
    22621 gagttctgta ttagataatc atctttctag gcctattgtc accaataggc tcaagcgacc
    22681 tacctaccgg agagtacgag cagcactctt tattcctatc ttggtcttgc tccaggtggg
    22741 gtttacatag ccaactagtc gcctagctgc tggtgagctc ttacctcgcc tttccatcct
    22801 taccacaata gtggcggttt atttctgttg cactatcctt aggatcgctc ccactaggcg
    22861 ttacctagca ccctgctcta tggagctccg actttcctca tgtaagatta catgcgatta
    22921 tctggcttac ttaatattta ttttacttag ttatgttaac acaaattctt ttacatgtca
    22981 aagtaatatt atactaaaaa tttaaattta ttcaaaaata gtgtaatata gatatataaa
    23041 ctacatttaa ctcgaggtga tctattgaaa aaattatata gaatagtaat taacattata
    23101 ctagtattag taatacttta cagtggattt aatatatact caaaacttac caaatacaat
    23161 catgatacta aaatatcctc agaacttcaa aaaaaagaat ataaaaaaga ggatttatca
    23221 aagattaaca gtgattttaa attttggctt tctgttgaga atacaaatat aaattatcca
    23281 gttgtacaat ctaaagataa ttcttactat cttgacaaag atttttataa aaaagattct
    23341 atttctggta cactgtttat ggattataga aataaatcta ttgatgacaa aaatatcata
    23401 atatatggac ataatatgaa aaataaaact atgttcaaca acctaaataa gtttaaagat
    23461 gctgacttct ttaaaaaaaa taataaaata aaaataacct tgaatggaaa agaattttta
    23521 tatgatgttt tttctgctta tattgtagaa tctgattatg attatctaaa aacaaatttt
    23581 aacaatgaat ctgattatca aaattacata aatgatatta cttctaaatc attgtataaa
    23641 tctcctatta aggtaaatag caatgataaa atagttacac tttctacatg tacctatgaa
    23701 tttgatgatg ccagaatggt gattcatggt agattgattt aaaactcaca gacacagttt
    23761 aaaacaataa ttttaaactg tgtcatcttt tatacctata atttgtaaat tagaccacta
    23821 aaagtattta atacaaaata acaaatacat caaacaataa aaaacaataa aaaaagacta
    23881 tgctaaaaac atagtcttta ctggagctgg tgataggaat cgaacctaca acctgctgat
    23941 tacaagtcag ctgctctacc gttgagccac accagcacac ctattttatt gtaaattagc
    24001 aaaactaatt tttaaaagtt a
//
LOCUS       DAMJWS010000069        23414 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMJWS010000069 DAMJWS010000000
VERSION     DAMJWS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.069"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(2..766)
                     /locus_tag="PC399_002683"
     CDS             complement(2..766)
                     /locus_tag="PC399_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2593533.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            1040..1714
                     /gene="fsa"
                     /locus_tag="PC399_002684"
     CDS             1040..1714
                     /gene="fsa"
                     /locus_tag="PC399_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2593534.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1836..3230)
                     /locus_tag="PC399_002685"
     CDS             complement(1836..3230)
                     /locus_tag="PC399_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF2593535.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFTFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3308..4654)
                     /locus_tag="PC399_002686"
     CDS             complement(3308..4654)
                     /locus_tag="PC399_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2593536.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      4904..5006
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5100..6476
                     /locus_tag="PC399_002687"
     CDS             5100..6476
                     /locus_tag="PC399_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2593537.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(6590..7735)
                     /locus_tag="PC399_002688"
     CDS             complement(6590..7735)
                     /locus_tag="PC399_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2593538.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            7988..8695
                     /locus_tag="PC399_002689"
     CDS             7988..8695
                     /locus_tag="PC399_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593539.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            8742..10337
                     /locus_tag="PC399_002690"
     CDS             8742..10337
                     /locus_tag="PC399_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593540.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMLIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            10440..11522
                     /locus_tag="PC399_002691"
     CDS             10440..11522
                     /locus_tag="PC399_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2593541.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            11671..11832
                     /locus_tag="PC399_002692"
     CDS             11671..11832
                     /locus_tag="PC399_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF2593542.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            12173..13441
                     /locus_tag="PC399_002693"
     CDS             12173..13441
                     /locus_tag="PC399_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2593543.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            13636..14424
                     /locus_tag="PC399_002694"
     CDS             13636..14424
                     /locus_tag="PC399_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2593544.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            14424..14873
                     /locus_tag="PC399_002695"
     CDS             14424..14873
                     /locus_tag="PC399_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2593545.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            14877..17141
                     /locus_tag="PC399_002696"
     CDS             14877..17141
                     /locus_tag="PC399_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2593546.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVK
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGRMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            17281..17592
                     /locus_tag="PC399_002697"
     CDS             17281..17592
                     /locus_tag="PC399_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593547.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            17878..18378
                     /locus_tag="PC399_002698"
     CDS             17878..18378
                     /locus_tag="PC399_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF2593548.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            18435..19421
                     /locus_tag="PC399_002699"
     CDS             18435..19421
                     /locus_tag="PC399_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2593549.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            19471..20193
                     /locus_tag="PC399_002700"
     CDS             19471..20193
                     /locus_tag="PC399_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF2593550.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            20222..22156
                     /locus_tag="PC399_002701"
     CDS             20222..22156
                     /locus_tag="PC399_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2593551.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKQEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            22149..>23414
                     /locus_tag="PC399_002702"
     CDS             22149..>23414
                     /locus_tag="PC399_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF2593552.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLILLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
BASE COUNT         8990 a         2507 c         3789 g         8128 t
ORIGIN      
        1 cttataaaac atttacatta aattctgata aaatatctac tatatcagga gtaggcgttt
       61 tttctgtaat tatagaacta atatcttcta aatgagcaaa cttgaaattt ccatcaaatt
      121 taaacttttt atcttccata actataaata cttttttact tgaatttatt atagccttct
      181 tcgtaagacc atcttcaatt tcaaaagttg taagtgaaga gttgaataca tctactccac
      241 aacttcccat aaaagtctta tcaaacttat atttcgatat aaactctata gttggagtac
      301 cgacaaaacc gtctaaactg agatttaata aacctccagt ggaaattata tttaaattat
      361 ttggttttga agtaactaca tttagtatat ccagcatatt tgtaacaagt gttacttttt
      421 tattactttc agctaacaac ttcgcaagca agatgttaat tgtagagata tctaaaaata
      481 tagtttctct atcttcaatt aaattaaatg ctttttctgc tattattttc tttgttggaa
      541 tattaatttc ttttctgtct tttgtatcct gattctctag tgtttctcta gctagtacag
      601 ctcctccata aattcttctt attgaagaaa tcttttctat attttttaaa tcttttctta
      661 tacaatcttc tgtaacatta aatttagaac tgagctcttt gacagtaact ctaccttctt
      721 tttttaggat atttaatata gcttggattc tttcttctgc aaacattttc tacctctttt
      781 cgcaatatag tattatagta aatattataa gataaaatac aatatatcaa ctattttaac
      841 atgaaataca aaattaattt aatataaaaa gtatgttttt tctaactata tatttagaat
      901 ttagtaaata aaaaaattat aaaactaaga aggaaaagta aaacaataat aaaaaataaa
      961 aaacaataag caaaatgttg aaaataatac caaatcaata tataataaga ataaagaaag
     1021 attaaagggg gatattttaa tgcaactaat cttagataca ggtaatgtgg aggagattaa
     1081 ggagttatgt acttgtttac caatagatgg tgtaactaca aatccaagta ttgtttcaaa
     1141 agaaaaaaag aattttaagc aacttataaa tgaaattggt gagattatag gtgaggatat
     1201 gcctatacat gctcaagtgt taagtacaaa gtatgaagaa atattagaag aagcattata
     1261 tataagtagt ttgagaaaaa atatatatgt aaaaatacct gtaacacaag atggtttaag
     1321 agctataaaa gaccttcata gaaaaggtgt aaaaataact gctactgcaa tattcactgc
     1381 tcatcaaggt tttttagctg caaaggctgg agcaaattat attgcacctt atgtaaatag
     1441 gcttgataat atatctggtg atggagtagc tatggtttca gaacttataa aaataataga
     1501 tacttataaa atggatacaa aagtacttgc agccagtttt aaaaatgcac aacaagttat
     1561 agaattaatg caacatgggg tgcatagtgt tacagtacca tatgatattt gtaaatctat
     1621 gatgaatcat ccattaactg attggagtgt agataagttt atagaggatt gggagaatac
     1681 ttttggaaaa ggaagtaaaa caaataatat ttagtattat ggtaaaagag tagttttgtc
     1741 caattttaca agaacttata taaaaagatg aaaatatgaa ttttcttttt aataatttcg
     1801 ttattatctt aagttaattc atattttcat cttttttaga tttctagtcc tataaatttc
     1861 caccaaggga atgttataca tattattata aagtaggtta aaggaatcaa agttaaaccg
     1921 tatttgaaaa taaccttgct attatacaga ttttcaccat gaccaaccat tattgtagca
     1981 tgatgaaatg gtaatatgta ctgtatgctt actataatgt agacaaatag acataaagta
     2041 acactatttt cctttagtat atgtaaactt ccaagtaatg gtattactac agatgatgta
     2101 gtgacagcac ttccaagaca tatattcaag attattgtga gagttatcaa aaacaatatt
     2161 gaaataattt ctgaagagtt tggtataaac tttatcaaat aatttcccat tatatctaca
     2221 actccagagt actttaacac tcctccaatt gaaaatgctg ctataaaaaa aataaggacc
     2281 ttccaattta tttcttttaa agtactaaat ccaactattt ttcttagtaa taaaagaagt
     2341 acacaaattg acattataat taatgtgttt atgttatgta tgaactgagt taaaaacata
     2401 aaaaacatta ttcctaggca taaaaatacg tgtttttgtt ctttgtttag caaattaaat
     2461 tcttttttta aattagaata tgaaaaatga agtttattac cctcaagatt ttcattagaa
     2521 ataaatttta ttgaatgcaa ttctttttta aagacaaagg taaataaaaa ataagttata
     2581 aaacaagtaa ttatagttgg aacagacata taaaaagccc attgacccca atttatatta
     2641 caatttccaa gttctagtac aacataattt agaagagtgt ctccatttac aaaaaacatt
     2701 gatgtaaagg tagatgctgt aaatatacta aataaaagta tagattttga atcttcagtt
     2761 atctgttgtt cttttaaaaa ctccttgtaa aaagcagata caagaataac ccttggaaat
     2821 ggttgtggga taaaaaatat taaaagtata cctaaaatat acgataaaag aatcattttt
     2881 ttaggggtat ttacagtatt taaaattaac ttctcagaaa taatttttgc gacacctgtt
     2941 tttgtaactg cattagtaat tatgtatgca agaattatta tgtaaaaatt ttctgtaaat
     3001 aaaaactgta aaataacctt tattggtgca atgttaaata taaacgctag aattacaaaa
     3061 aacaatgcaa caaaagtttt ttcaactgag tctacagccc atatcaagat agttaaaaaa
     3121 gataagcata tagaaaattt tatgttgaaa ctgtaattaa gagagaagta gagaattatg
     3181 gtagctataa cagtcatata aaattgtttt ggcttaagaa taatacccat ttttatattc
     3241 acctccataa tttattatat cataatggag gtgaatacaa tgaatgtgaa tacattttat
     3301 aaaaagatta ttcattgatt ctatgattta caatagcttt agttttccaa tgtcctttct
     3361 tatagtagat aactgtaaca ataagaccta aagtccaacc aaaaggaata cccatccaaa
     3421 ttccgtctgt tccaaaaaat ttagaaagca tataagaagc tggaagtctt ccaagccata
     3481 aagatactat tgagcttata agtgcaaaca tggaatcacc agcacctttc attgcacttg
     3541 aaagtacaaa agaagttcca ataaataaga agaatggtcc tattataaat aagtaagaag
     3601 agccaatttt aatgacatct ttatcagtat tgaatagagc aattagattt tccctaaata
     3661 gcaaaattaa agcaataact gtaactgcta aaactaaggt catttttata gattctcgaa
     3721 tgcctttttt aactctttca ttttctccag cacctatgtt ttgagcaaca aaagtagaaa
     3781 cagcagaacc aagattcata ataggtaagc ttataaaatt ttctatccta agaccagcac
     3841 caaaagctgc catagcagat gtaccataac tattaactaa aaattgaaga gccatatttc
     3901 caattgaaaa caacatttgt tgaactccac ttggaagtcc taatctaagt ccatctttaa
     3961 atattttgtt atcatataca attcccttga ttttaaattt aagcacttca tgttttttat
     4021 ttaaataaat tatacttatt ataaatgata ctgcctgagc cattatagtg gcaagtgcaa
     4081 cgccctcaac acccattctt agcactacaa taaaagttaa gtctaaaact atatttagaa
     4141 ttgtggctat tatcagaaaa taaagtggag ttactgaatc tccaagtcct ctaagtattg
     4201 cacttactga attatatcca gcagaaaata atattccact aaatattata gtaagataca
     4261 attttgattg agcaaaaacg gattctggtg tattcataag cttaagcatt gggccaccaa
     4321 atactattcc aagaatactt attaatatag aaccaataaa taaaagtata tatgtagtat
     4381 caatagtagc ttttaccttt tttaaatctc ctgagccata aaattgtgac actaaaatag
     4441 aaaatccaag gctaacaccc ataagcgctg ccaccaataa aaacattata gggttagctc
     4501 cagagactgc cgccattgct tcttttccaa taaatctacc aactattata ctgtcagctg
     4561 tattgtatag ttgttgaaat aaactaccaa taagcatagg catggcaaaa aagaatatgg
     4621 actttccctc atgacctgta gttaaatctt tcaccttatt cctccaaata aaaaaattat
     4681 taatttatta tagtataatc ttaacataaa ctttatatcc aaactataaa taaattgtat
     4741 caaaatatca tatatttttt taaaatgtaa agaatataat tataatttga atttaataaa
     4801 tcaatattta atataatcga atattaatac aaaatcaata caatatatac gtatatattg
     4861 acaaaattaa ttatataaat taaaataata ttcgtaaaga aaaattcatg ttttgtactg
     4921 tataacatat aattttgaca atatgggtca taagtttcta ccggaatacc gtaaatattc
     4981 tgactatgta tactattatt tagttaatta aatatttaat taactgaatt atgaaggata
     5041 cggctcagct tatcaactac tgtgtcgttt ttttgtaaaa tttaatagga ggtgttgtta
     5101 tgcaaacaac aaacagtagt actatgcaga aactttttcc aattttgact aataaaaatg
     5161 tcgacatgaa gaaggaaata attgcaggag taactacttt tttaactatg gcttatataa
     5221 tagctgtaaa tccaaatatt ttatctgaga caggcatgcc tgcaggtgca cttgttacag
     5281 gtacttgttt agcagcagca tttggatgta ttttaatggg tgtagttgct aaccttcctt
     5341 ttgctttagc atcaggtatg ggtcttaatg cattttttgc atatacagta gttttacaaa
     5401 tgggtgttcc ttgggaagta gctcttacag cagtatttgt agaaggtatt atttttatag
     5461 tattatcagt aagtggagtt cgtgaggctg ttgtaaatgc gataccaaaa aatatgaaat
     5521 tagctgttac aggtggtata ggaatattta ttgcacttat aggtctagtt aacagtggaa
     5581 ttgttattgg agaccaagcc actcttatca aaatgggtag atttacacca gcagtaataa
     5641 taacttgtgt aggattaata attattgctg tattagataa aaaaagagtt aaaggttcta
     5701 tactttttgg aatagtagtg agttcattac ttgcttgggc atttgctttt ataaatccag
     5761 agtatgcaca aaaattaggt atatatttac ctggtggatt atttaaattc gaaagtttag
     5821 caccaatagc aggtaaaata gatttaggat atgtactaca tccaacaaat ataggtggtt
     5881 ttttagtagt agtttgtaca tttttatttg ttgatttttt tgatacagta ggtacattag
     5941 ttggtgtttg ttctaaagca aacatgttag atgaaaaagg gaatgttcct aatgtaggta
     6001 gagccttact tacagatgcg gttgcaacta cagttggtgc aggacttgga gtatctacag
     6061 ttacaactta tgttgagagt tcaacaggag ttatcgcagg tggaagaact ggatggacag
     6121 caataacagt tggaatttta ttcttagctg caatgttttt ctctcctgta tttatagcaa
     6181 ttccatcatg tgctacagca ccagctttaa tatatgttgg atatttaatg cttggtacag
     6241 ttaaggatat agagtttgat aatataactg aaggtgttcc agcttttgtt actattgctt
     6301 gtatggcatt aacatatagt ataggtgatg gtttaacttt aggtatatta acttatgttt
     6361 tcgtaaatat attttataat atatttggag ctaaaaaggc agaagacaaa aaacatgttt
     6421 ctgtagtaat gattgttctg gcaatattat ttataataaa actttggcta ctataatagg
     6481 tattatttaa aattttaatt taatataaaa taaagggctg taatttatta tcaattgtga
     6541 tgataaacta cagctctttt gtatttgttg tatttttgta tttattgtat tatttaataa
     6601 tgtctattag ttctccattt tgtaaagttt taggtgtaat tactatattg aactcttttt
     6661 gcaaatcttt tataatatct aattcttttt ttgttattat tgagattgtg ttaccacttc
     6721 gatttcctct agcagttcga ccacatctat gcaggtattc atttttactt tttggaaaat
     6781 ccaagttaaa aacatgactt acatccacaa tatctagtcc acgagcagat aaatcagttg
     6841 taattaaaat ctttgcttta ccaagtttaa atttatttat tgcattcttt ctatcttctt
     6901 tttccatatt tccaaaaatt cctattgctt tgtaattatg ataatttagt tttgatacta
     6961 ggacttctat atttttttca ttatttacaa atacaatagc tttttttgga tttgtagctg
     7021 ctaatgcctt tctaagaaaa gtaaatttat cccttatctc acctaataaa tatgagtgat
     7081 tgatgttagg atttatttgt gatttttcct ttgtttttat tatttcaaac tccttcataa
     7141 gtttatcaca aatttttata gtgctatcag taagacttgc agagcatcct ataatttgtc
     7201 tatctcttaa cgttgttctt attatgtctt ctatacaagt tatatttttt ccattcaata
     7261 gattgtccac ttcatctaag acaatagttt ttatattatg tgattttagt ttcttttgtt
     7321 ttatcaagtc taaaactctt ccacaagagc caattactat gtgtggtttt actgctttta
     7381 tattttttat ttgtttttgt atattgacct ccccaattaa agctaaagaa gtaacggaga
     7441 gttctgcatt tttagccaat aattcaacct ggtttgtaat ctgcattact aattcatgcg
     7501 taggtgcaag tataagagct tgtgtttctc tttttgatgt atcaattttt tcaaatatag
     7561 gtaaaaggta cgctagtgtt tttcctgttc cagtctgtga attaatcaat aaatccttat
     7621 tttgaattat attccctata acaagtgact gaacttctgt tggagaagtt atgtcttgtt
     7681 ttttgagtcc atctattaga gtggaactta tttttaattg ttcaaatgta ttcatatttt
     7741 actccttata ttttaataat tatagtcaac tattttttat tatacattgt ttattcaaat
     7801 cgaactatat attcttttat aattatgtgt aatttgaaag taaatatcaa ttatatttat
     7861 acctttatat ttctgatacc tttgcatttt atagatttct aaaataaaca aataaaagtt
     7921 gtaattgttt gatatttgtt atatattgta tataacaaat atcaaaacta taatatggag
     7981 ggaaattatg ttaaaagtaa tcaatgtatc taaatcatat aaaaataaaa aagtagtaag
     8041 tagtgtctca tttgatgtaa atgatggaga gctttttggg tttataggac ataatggagc
     8101 aggtaaaact actactatta aagcaattgt tggaatacac gattttgaag agggagaagt
     8161 actaataaat tctaaatcta taaaaaaaca gcctgttgaa tgtaaaaagg aaatggcata
     8221 tatacctgat aatccagatt tatacgagtc attaactggt atacaatacc ttaattttat
     8281 agctgacata tttgaagttg ccaaagataa aagagaagag ttaataaaat attattcaaa
     8341 taagtttgaa ctcaataaag cattaggaga tttaatatct tcatattcgc atggtatgaa
     8401 acaaaaatta gctataattt cggctttgat acacagtcca aaaatcttaa ttcttgatga
     8461 accttttgta ggtcttgacc caaaagcttc ctttatattg aaggaaatta tgagggagtt
     8521 ttgtgacaaa ggtggttgta tatttttctc aacacacgtt ttagaagttg ctgaaaaaat
     8581 ttgtgataag attgccataa taaaagatgg aaatataatt gcttatggaa ctacagaaga
     8641 agttaaggga agtaattcac ttgaaagtat ctttatggaa ttaatagaga aataaaatgt
     8701 tttttataca atatagtgtc taaagtgagg aatgataaaa tatgagcaat ctaagtatat
     8761 taataaaaac taatataata aatgaattta aattaaatgc gttaaaaaaa gctgatggta
     8821 gagaaaaaca aaaaataatg ggaatgatgc tttcagttgt tataatagcg ggtctattaa
     8881 ttttttatgt aacaagctta agtctctctt tagttgatat cttaaagcaa ataaatcaac
     8941 tggaaatgct actgatattt ggttttggat tatcaacatt aacaactatt gttacatcat
     9001 tatataaaac gtcatcatat ctatttcaag caaaagattt agaccttttg actagtttac
     9061 ctataaaaga atctactgta ctagcaagta agatattaat gttggttgga gcaaactatt
     9121 tattttcttc aatatgtatg cttatacctg cagtggttta ctttttaaat tctgatgtaa
     9181 gtataactta tttcccttat ttgatattgc tatttttagc tttaccatta ttaccgatag
     9241 ttgcttcatc aataattttt ctatttatag ggcagatatc atcaagaata aaatacaaaa
     9301 atttagtatt aatcataggt agcattatat tgatgttgct ttatacccta cttatgtcaa
     9361 gacttaattc tatgtcagtt gaaatgctaa agaatagtgt gtctatttca gagacgatta
     9421 gaaaagtata tcctcctata tattattttg ttgatgctct taaaacagga agtatagttt
     9481 catttttagc atttatagcc atctcaataa taccattttt tatgtttgta actctgtttg
     9541 caaaaaaatt taaagatata aatgctaaga tgagtgaagg atataagtca aaaaattatg
     9601 aatttaaggg tcaaaaaact tcaagacctt caaaagcttt attaaaaaaa gaatttaaga
     9661 gatatacttc ttcgtttata tactttttaa attcatctgc aggtttgtta tttctaatag
     9721 taggtacaat tttaatatta ttatttggag cagataaagt gggtgagtta ctgaaattga
     9781 atatagattt taatcttata aaaatacaat tattgggagt attcttcttt ataatagtaa
     9841 tgaattgtac aacatactgt tcaatttctc ttgaaggaaa aaacttatgg atactgaaat
     9901 cttctccaat agatgaaaga gaaatattct ggtcaaaaat attgatgaat tttttattga
     9961 atgcaccact atctatttta tgtctaatat tagtatctat aagattagac tttgaaattc
    10021 agtttattat tgtatcaatc tgtttgattg taagtttttc aatgtttgta gcattgatgg
    10081 gattattatc aaatttatta tacccaaacc ttgattggaa aaatgaggtg gctgtagtaa
    10141 aaagaagtgc aagtatgata gttacaatgt tagtatcatt agcttatgta attatacttg
    10201 gaggtatata tgtattactt ggaatttcgt ttttaaatat atatattatt ttggcaagta
    10261 tacttacttt gatattagat tttatgatat ggagaattat ttctactaaa ggagtaagaa
    10321 tatttaaaaa tttataggtg tatttatgtc aaataaagac ttatcttttt tagtcttgca
    10381 aataataggt taagtagtat actatgtata gtgatatgaa aaaatttagg agaaaataaa
    10441 tgagaaaatt atccaaaaga gaaaaagatt ttattcaata tggattttta ttatttttga
    10501 tatgtattac tacttattta gtattaacta ctttagatat aaaactaata cctaaaatta
    10561 taaggctagt aaataagact tatatatttc ttggcatatt gacaattata gtatatatga
    10621 taattgaatc tattgtaact catttgataa taaatgcagt tcaaaaaaca aaagtaaaat
    10681 ttattggtat taaaatggct acaatgggat tttactataa cttagttact ccatttgcat
    10741 ctgggagtca accaatgcaa atatatgcac ttacaaaata tgatgtaagc cttagtaaat
    10801 cagtggctat agttactaat aaaactgtag tatttcaaag tacagttact gtattttgtg
    10861 gaattttaat tttgctaaat aagtcattac tgatggagca agtacattct gtgaaagtct
    10921 tgataacttt tggaatgatt atgaatgtaa gtatgttgtt aggtggacta ctaatagtat
    10981 ttagtccaaa acaagtaaaa atgatagcag aagttttgat taatttttta tctaaattta
    11041 aaacatttaa atttttagat tcaaaacgtg atagaataaa tcattatatt gatgattata
    11101 attattctat aaaaatattt attaaaaatg taagaatctt agttttaagt atattactta
    11161 ctgtgttaca attaatcgca tatttcagta tagtttattg tgtgtataaa gcctcaaatt
    11221 taagaggagt ttcatatgta catataatga cattacaggt ctttttatat atggctatat
    11281 caccaattcc aacaccagga aatgttggag ctaatgaggt tacatttttt actatgttct
    11341 ccaatataat tccaaaagaa ttattggggt actctgtact cttatatagt gggtttgtat
    11401 actattttat attaatagtt tcaggtttat ttacatttgg aacgcattat acattaaatt
    11461 cttggaaaaa ggaaaataaa gtaaattcta atttagataa ggttattgaa aaattaaatt
    11521 aataaaataa ttagggaaat tctatatata aatatggtgt tgaataagct atattatatt
    11581 gagatatttc ccttttttga ttaaaattat agaaatttga aataataagt aataaacaga
    11641 aaaagtgata ttgttttgaa aggagaaact atgaaacaag ataaacaaaa tgaaaaacaa
    11701 agtgagattt ctaagataaa acaaagcaat gataataaac ttgaaatagc tcaagaatta
    11761 catgtaagta aaaatatagg gaatatgctt ggatatagaa tagataagaa aaaaatgaat
    11821 agaccaagtt aatgaaaatg ttgaaaatgg tacattttaa aaaatatata taatgaaatg
    11881 ttttattgtt aaatttaaac ttattttaat gggggaaaaa gtcgaaaaat atctaaaaag
    11941 atatggatat aaaaaatatt aatatatata tactataaac ttgacaaaat caacctttta
    12001 ataactagca aaaaatagca aaattatata agcgttattt aattgtcaaa aatgaaagct
    12061 aaattgcatg tcctaaaaaa aatgattgat aacatttatt tgtgtgctat aattatatag
    12121 taaaacaata agtatattgc ataatgtatg caaatcggga ggagagacgg acatggaagc
    12181 atctatacac acaattgcca gcaataattt attgataatt tttgctattg taagtataac
    12241 tggaataatt tgtagtaagc ttagtgaaat aataaaggtt ccagatgtag tgttatattt
    12301 acttgtaggt attttaatag gaccatcatt tttaaaattt atagatattc gtggatttca
    12361 aatagaaaat caactaatac ttacatttgg ttctgcattt attttatatt taggtggaaa
    12421 agaaataagt ttaaaagtat tgagaaatgt gaagatatct gtattcttat tagctacttt
    12481 aggagttgtg atatctgctt ttataatgca acaagtaata ggatttactt ttgggataag
    12541 tgcaatgact gctttactag caggaactat aatagcatca acagacccag ccactcttgt
    12601 acctatattt aatcaggtaa agataaagga tagggtaaaa caaactgtaa taagtgaatc
    12661 agcttttaat gatgcaacag gagctatact aacatcagca gtgttggctg taattttgtc
    12721 aaataaattt tcattaggtg aaaatattta tgaactgggg acaatggtta ttgtcggagt
    12781 tttagttggt ttaattacag gaatactgct tttgaaatta gttaatgata aaccttatgg
    12841 aatatttaaa gattttgcac caataatatc tgttatttca gttgtagttg catatgaatt
    12901 atctacaaaa ttaggtggaa gtggatatat gtcttgcttt attgtaggta taataactgg
    12961 taacaagaaa aactttaaaa tttggttaag tcaaaaatct tatgatgcag atttttatgt
    13021 tgctgaaact ttaggtacta tttgtcgtat ggcaatattt ataatattgg gtagccaagt
    13081 taatttggct cagttaagta aatattttat gccatctgta attacagtat tagtattaat
    13141 gtttatagca agaccacttt gcgttttagt ttgtacactt gtagacaggg aagcaaaatg
    13201 gagtaaaagt gaaatattat ttatgatgtg ggtaagggaa acaggcgtaa taccagcagc
    13261 actttgtggt attatttcag caatgaaagt tcctggatac gaagtgattt catctgtagt
    13321 atttatgact atactaatta cattagtaat acaaggaagt actacaaaat tagtagctaa
    13381 aaaacttgga ttattagaag aagaagtgat aaatataagt gaaaaagtat caacttttta
    13441 aagttttgct ttaaaatagt acctgttaat ggctatttta atattgtcta atgatgatta
    13501 ttgaaatagt cattttttta tgtataagta atttacagaa tttacatatt agacatattt
    13561 attatcaaaa tttgttgaag ctatgatata attagttgta tttaaaataa gaatagcaaa
    13621 taagggagga aatttatgtt tacggttatg gatattgtac agccagatac agtagaagaa
    13681 gcgtactcaa ttttaaataa aagaaaaact aaccaagtaa ttggtggaag tgctttttta
    13741 agaatgggga aaaaaagaat aggaacagga atagaattat ctaaattaaa tttagattat
    13801 ataaaagagt atgaagatta cgttgagatt ggttctatga ctacttttag aacattagaa
    13861 acaagttcca taataaaaaa taattttgga agaataatag aagattcagt taaaaatata
    13921 ataggagttc agtttagaaa tgtagtaaca gttggtgcaa cagtattttc taaatatggt
    13981 ttttctgact tgatagtagc attactttca ttagatacag aagtagaatt atataatatt
    14041 gggagaatta gtcttgaaga gtttctaaat agagattatg agaaagattt acttattaag
    14101 atatacataa agaagacaaa taagaatgct tcttataaat cacttagaaa tgcaaagagt
    14161 gattatccaa ttctaaatgt atctgtatca aaacatatgg aacaatttaa gttatgtgta
    14221 ggagcaagac cgcagaaagc tacgattgca aaacaagcta gtgaatttct atctaacaat
    14281 gaaatcaatg aaattaatat agataaagca gtagaaatag catcagaaga actaactttt
    14341 ggttctaaca tgagagcatc tagggagtat agaaaagcta tgagtaaagt acttttaaaa
    14401 agagctataa tggaggttat ttaatgttag taaagattaa tataaatggg aagaaaaaaa
    14461 gtgttgatat agaaccagaa gaatatctgg ttgatacttt aagaaaagtt ggtaatttaa
    14521 gtgtgaaaag aggttgtgac acaggctgtt gtggtttatg tacagtttta attgacaaga
    14581 aacctacatt atcctgtgca acactgacat tgagagcttt agataaagaa ataactacaa
    14641 ttgaaggttt agaaaatgaa gttaaagaat ttagtgaagt tttggtaaaa gaaggagctg
    14701 agcaatgtgg tttctgttct cctggcttta tacttactgt acttgcaatg aaggatgagc
    14761 ttgaaaatcc tacagatgat gaaataaagc attatcttac aggaaatcta tgtagatgta
    14821 cagggtatat gggacagtta agagctatca aaacttatct ggaggtaaaa tagaacatga
    14881 atatagttgg aaaaggaatc aagaaaatag atggaatgag tatcacaaca ggaaagcctg
    14941 tatatacaga tgatttatca gcaaaagatg ctttggtggt aaagatactt agaagtcctc
    15001 acgcttatgc aaaaataaaa gatatagata catcaaaggc agaaatggtt gatggtgttg
    15061 aatgtgtttt aacatataaa gatgtaccaa aatctcgatt tacacttgct ggtcaaacat
    15121 atccagagcc atctccatat gatagattaa ttcttgaaga tgttgtaaga tatgtaggtg
    15181 acgaagtagc tatagtggca gctatagatg aaaaaactgc aattaaagca atgaatctta
    15241 tcaaagtaaa gtatgaagta cttacacctg ttttggattt tgaagaggca ataggaaata
    15301 aaactatggt acatgatgaa gatagacatt gtaactttga tataggtatg caaagagaaa
    15361 aaaatatagt atcccatcat gattatacaa aaggtgatgt agaaaaagta ttaagtaatt
    15421 gtgatgtagt gattgaagaa acttattata ctcaagctca agcacatgca atgatggaga
    15481 cgtatagggc atacaattat ttagaccata tgggaagatt ggttgtagta agttcaactc
    15541 aaataccatt tcatgtaaaa agacatttat ctcgtgcact agaaatacca gctagcaaaa
    15601 ttagggttat aaaacctaga attggtggcg gatttggtgg taaacaaact gcatgtgttg
    15661 agattttttc tgcaattgtt actttaaaaa cagggaaacc agctaaaata atttatgata
    15721 gaaaagaaac ttttaattgt tcaacaagta gacatgcaat gagattaaaa gttagactag
    15781 gtgctacaaa tgatggcata ataaaggcaa tagatataaa agcactttct gatacaggag
    15841 catatggaga gcatgcttct acaactattg gtttggttgg agaaaaaact ttaccaatgt
    15901 ataataaagt tgaagcaaca agatttattg cagatgtggt atatactaat aagatgccag
    15961 caggagcact tagagggtat ggagcatcac aaggatgttt tgctgttgaa tcaactataa
    16021 atatgcttgc taataaatta aaaatggacc caacagattt aaggttaaaa aatgtggtaa
    16081 cagaaggtga aactacattt gcatatggta aaactttaaa tagtgtcgat ttaataaaat
    16141 gtataaataa aggcaaagaa cttattggat ggaatgaaaa atatccttgt aaacatcttg
    16201 ataatggaaa aattagaagt gtgggtatgg caattactat gcaaggttct ggtatacaag
    16261 gtcttgataa gtcttcagct gaaataaaat tgaatgactg tggagattat actcttatga
    16321 taggttctac tgatatgggt acaggaagtg atacgatttt agctcaaatg gctgctgagg
    16381 ttcttgatac aagcattgaa aatataacag ttatatcagc tgatactgac ttagttccat
    16441 atgacccagg ttcatatgct tcaagtacta cttatgtaac aggtatggca gtagttaagg
    16501 cagctcaaga cttaagagaa aaaataattt ctgctggagc aactaaacta ggtgttgaga
    16561 aggatgaagt aaaatttgat ggtataaaat taactagtaa aaaaggtagc actttattat
    16621 tcgatatagc agttgattct acaattggtc cagatactaa tcaattaata ggtcttgctt
    16681 ctcatggtag cccagtatct cctcctcctt ttatagcagg ttttgcagaa actgaaattg
    16741 acacagaaac tggaaaagtt gatttagtaa attatgtagc tgtagtagat tgtggaacgg
    16801 ttataaataa aaatctagct aagatacaag ttgaaggtgg aattgttcaa gggattggaa
    16861 tggcattatt tgaagaagtt agatacacag aaaaaggaag aatggacaca aatacattta
    16921 tgcaatacaa gattccagct agatgtgata ttggaaatgt taaagtagat tttgttgaaa
    16981 cttatgaacc aactggacca tttggtgcta aatctgttgg agaagttgtt ataaatacac
    17041 ctttaccagc aatacaagaa gctgtgttta atggaattgg tgtaagaata aatacactcc
    17101 ctattacacc tgaaaaagtt tttatggaaa tgaataaata attatttatg aaaatttaga
    17161 ctagttttac atgttaaaca accagttatg tcatacctgt tgaaagatat ttaataagta
    17221 gttattataa tattatgaat tttacttata aaatacaaaa ttaaataagg ggatgatgtt
    17281 ttggctggta atctaaataa tatgagagca gtaaataatt ttagaggaga taagaacatt
    17341 ttagaatgtt tagtcagctt tgagggtcgt tcaataagtc agagaaaagt aagggtattt
    17401 tttaaagaaa aacaaaatca aatagaaatt gattttgcag aagaggaaat ttctaaattg
    17461 gttgaaaatg ttgttttaaa tacatcatat caagaaatgt tatatgatga aatagagaaa
    17521 caactggaaa ttgattgtat aggtacttgg atgatattat ctaaattaaa agatggtagt
    17581 agagttcact gattttaaaa taaattatta gagcctaatt ttaaggctct tttttgctgt
    17641 ttttaattaa caatagaaaa cttatcttta gtttgtatat aattttaata tcatatattc
    17701 tatatatata aaattgaatt tgtatgtatt atattgtata atgtaaaatg aattggaaaa
    17761 atagttcgat aaaataattt gtttttataa aattactgtt taagtttata gattttgtat
    17821 aaaataaagt tatgttatta atttttttag aaataaaact tttactagga gacagatatg
    17881 agaaaaaata gtattaatat agcactaact gcagcaatta cagcaatcta tgcagtttta
    17941 acattatcat tgggatttat aagttatgga ccaatacaat ttagggtagc agagattatg
    18001 atgcttttgg catttttaga taaaggatat atagtaggtc ttacattagg ttgtttttta
    18061 gcaaatgtaa taggaccata tggtgtacct gatataattt ttggaacatt agccacattt
    18121 ataagtgcaa gtatgattta tattactaga aagttaggta aacagaataa aagtacttta
    18181 gttatagctt ctttatggcc aactgttata aattcgattg tggttggtct aatgttaaat
    18241 gtattctttg ggcttccatt aattctatct atgatacaag taggttttgg agaatttgta
    18301 gtagtgacaa ttgtaggagt accatttttc tgctttttaa tgaaaaaata taaaaatgtc
    18361 attaatatta aattttaaat agtgtatagt aatttaatta attgagagta ttattagtga
    18421 aaagaggtgt ttatttgtcg aaggtaaaaa gattgactga tgtttggata tatattaatg
    18481 ataagcaaaa gtttacaata aaagagttgt cagaggaatt taatctttca gctaaaacaa
    18541 tacaacgata tttaaaagaa ttaaataaaa tgggacttcc aattcaagca gaacaaggta
    18601 gaaatggagg ttatagggtg ctaaataata gctatatacc tcctgttata tttacagaaa
    18661 aagaagttat gattatacta tttgcactta agtcacttca gatttataat tataagttaa
    18721 tggaaataga aattaattct ataatgagaa aagtaagtta tgaacgtaaa agcagtataa
    18781 aagtaggtat agaaaatatt aaaagataca ttggctttgt agctaatgat actaatgatg
    18841 gagattttaa gagtagtaat gtaactgagt tttttagagc ttcttcagaa tctctaattt
    18901 taaatattaa gtataagtgt ggtaatcaga ttttggaaaa aaacgtatgc cctataggta
    18961 tatatttaaa taagggagca tggttttcac cagcatatga ttgtaaaagt gataaaataa
    19021 tattagttca tatagataat ataattgata taaaaagaac aggagaaagt aaaaaaatac
    19081 atataaactt ggaagaatgg ttacatgaag tatataacaa tttatatgga aatagttcaa
    19141 ctactaaaaa atttggtgaa attatttatc taaatgtatt attaactaaa gaaggtgttt
    19201 ctaaaataaa ggacagttct tggaatttag atatggagct aaatgaagat ggtagtggaa
    19261 taattagtac attcataaag gttgaagata tagattgtta tgcttcgatt ttatttttta
    19321 ttggaaataa tgctaaggtt atagagccaa aatttttgaa taagctactt tatgataagg
    19381 ccaatgagtt aagatatttt tatgaagaaa atatggtata atatttagaa atgttgaaat
    19441 taaataatta ttaaggagat atacgttata atggaaaatt ttaataagaa tgctaaaata
    19501 attggtattg gagctgaagg tattaatata ataaatgaag tagaagaaaa aataaaagca
    19561 aatatggata tagaaaaaat aaacataaat caagagattg aaaaagaata tgttagaagt
    19621 cttttagatg gtgtagatat attatttttg atatattcat ctgaagataa acatattaga
    19681 gatattataa aagctgtttc atatatgtca aacgaaagaa gagttctatc tataggaatg
    19741 gattgttcag agaaagaaaa taaagaagat ttagagcttg ggagagagtt taaaatcaat
    19801 aatgatagta tatttaaatt tgttgattta atgaatatca tggtagagtc tatttctgat
    19861 tcttgcatga taaatataga tattactgat ttaaaagaag ctattgtagg agataaagga
    19921 attaagtatt cttttgaaga gtttgaagat acaaagtcat atagcgagat agctgacatt
    19981 ttatttgaca ggatggaata tattggtgag gaatttataa gtaagaaagg aattgtattt
    20041 gttgaaggaa gtccagaatt ttctataatg gaattgaatg atttaataag taatattcag
    20101 agcaaggtag aagaaagcta tgaagtaata ttttctttat atataaagga aaatttaaat
    20161 ggaaatatta gagtaggctt attgtataat tagtatattt tagatattaa ggagagtgta
    20221 tatgtctgaa aatataagag gtacagagta ttggttaatg gaagaatttt tttatcagga
    20281 aacagacaca ccacagattt ttagagaaga aagcataatt gcacttggat ttgacataaa
    20341 aataaattta aaaaaagtat tcagtatggg gaaaaaatca gaaaagtata ttaatgagaa
    20401 ttgtaatgat aaagatttta taaaaaagtt tatgtcgatt aaaaaaggag attatttttt
    20461 atataaagca tatcaaaata tagaagaaaa agttaaatgt aatgtgtcta ttggcatagt
    20521 tgaagaagat tttgaaaatg gatatgaatt aagtcatatt ttagggcata cactaccagt
    20581 aaaatggatg cataattttg atacagtttc aaattgtaaa gtatatacaa gtgaatttta
    20641 taaattaaat aataatgata ttagctcata tttagaaatt attaagtcac aagataagaa
    20701 attattgaga aaaagaatag aagaaagtga aaataataaa tggtatttaa aaaactatta
    20761 ttataaaaat gtagataagt gtgtagatga tgaagaatta tttattcagc ccattaatct
    20821 agaacaatta gaaagcctaa aaaatataaa gacagatgat tatattttat tgtataatag
    20881 cgagaaagta atttgtgaaa atgaccatac aatgaagagt ctatttggcg tatgtcaagt
    20941 tatggaaggg ttcgataaag agaaagtaag tgctttggaa ggtggatatt tttttcctgt
    21001 tagatggata gagtatgaaa aggagaatat agacggaaat gcatgggtta tatttgaaaa
    21061 taatagtata gttgaaaagt atgtaaaaag atttactaat aaaatgccat taaatactat
    21121 actttatgga ccaccaggaa ctggtaaaac atacaataca aaacagatgg tttacaatct
    21181 agtaagtgaa accagtctaa gaaacaaata ctatacaggt tacttagatt ataaaaacaa
    21241 tgtagaattt tgtacatttc atcaatcata tggatatgag gaatttatag agggtcttaa
    21301 gtctgatggt gaaggcaatt ttaagcaaga agatgggata ttaaaggata tatctataga
    21361 agcatgttat aatgcattga aatttgaaaa aaagatgagc tttgagttgg aaaaagatag
    21421 cttgacttta gaaactataa aagacaggaa aaagaaatta gtattagaaa atatagaata
    21481 tagtgagagt tttaattttg aagaatcaga aaactatgtt ttaataatag atgaaattaa
    21541 tagagggaat gtatctaaaa tatttggtga gctaataaca ttattggaag atgataaaag
    21601 acttactaaa tcaaatcaaa ctattgtcaa actaccatat tcaaaggaaa aattttctct
    21661 acctccaaat ttgtatatag ttggaactat gaacacatca gataaatcaa tagcactttt
    21721 agatatagct ttaagaagaa ggtttaattt tgaagaaatt atgccagatt atgatttatt
    21781 gtctattgta gatggtatag atattagaaa aatgctatat acaatcaata aaaggataga
    21841 gtttttatat gatagagatc atgttattgg acaagcttat ctacttgctg tagaaaatat
    21901 taatgatata gtagtagtat ttagaagtaa aataatacca cttcttcaag agtattttta
    21961 ttatgattca gaaaaagtag gtttgatttt aggaggaatt ggaagaaatg aaaaagataa
    22021 atacattgtt tataaagaaa aacttatagc ttctgagtta tttaaaaata ataatgcctc
    22081 aataatggag agtaaagtta actattgtat aaaacaaaac atatctagtg aggagctaaa
    22141 aaatatatat gagtaaaaat atatatgtta aggaaaccta tgaatggata agggtaggta
    22201 atggtgaaaa tgagcttaca gaaattgaat atgaaaaatt gcttaaatat ctagagaaca
    22261 ataatgatgt attaaaaagt aatataatag atattaaata caaaaaactt agatttataa
    22321 actatgtagg catcatatgt tttgaaaatg taatacttga aatacttcct aaattgtcat
    22381 taagtgataa tcttgtaaaa gatagagaga tactattaca gatgctttct atatgtaata
    22441 aaattccaat tacaatgaat gaaaaaataa gattgagttt gaaaaattat aatctcttaa
    22501 atttttttgt tatgtatttc attgagagta tgcaaacaca gatgaaaaaa ggtatttatt
    22561 ttgaatatat aaataaaata gagaacttaa atgttatgag gggtaaaata ttattaagta
    22621 catatgcaaa agaaaaaggc atttctccaa tgaaaataag atgtgaatat gatgagtata
    22681 gtgaaaataa tttcttgaat caagttctta aaaaagcatg tatatctatt ttatgtagaa
    22741 taaatgacaa ttcaattcaa ggcaagatta aaaaaatttt aagctatttt caaaatgtag
    22801 atttaattta tatagataga aaaaaactat tggattataa attttataaa aacaatgata
    22861 gatttaaaga ttgttattta ctagctagac ttatactttt aaatttgtct atggataata
    22921 gtcaaaataa tcaagaggca ttttctatat tatttgaaat aaatactttg tatgaagaat
    22981 atataggaat tttaatcaaa agtatatggg acaattcatt tagagaaaca tatatacaag
    23041 ataagagtaa atttcttttg aaaaatgaac aaacaggtaa gaaaaacttt aatttaagac
    23101 cggatatagt tttatatgac ttaaaaaatg aatatgaaat tataattgac actaaatgga
    23161 aagctattga agtagattca aatgtttttt ataggtcaag tgatatatac cagatgtatg
    23221 catatataac agcatatgaa aatgcaaaaa gatgtatatt attatatcct tgtattcaaa
    23281 aagataagaa ttatagttcc tggaaattat cagaatcatt taagggtaag tttatagaag
    23341 caaaaactgt gaggttagat gatataaaaa acacaaaaaa tgatttaaaa aagataattt
    23401 ttaattataa attt
//
LOCUS       DAMJWS010000070        22725 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMJWS010000070 DAMJWS010000000
VERSION     DAMJWS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.071"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            42..506
                     /gene="bcp"
                     /locus_tag="PC399_002703"
     CDS             42..506
                     /gene="bcp"
                     /locus_tag="PC399_002703"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF2593553.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            516..1259
                     /gene="yaaA"
                     /locus_tag="PC399_002704"
     CDS             516..1259
                     /gene="yaaA"
                     /locus_tag="PC399_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF2593554.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            1387..4044
                     /locus_tag="PC399_002705"
     CDS             1387..4044
                     /locus_tag="PC399_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2593555.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFAPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4098..4200
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4310..5575
                     /locus_tag="PC399_002706"
     CDS             4310..5575
                     /locus_tag="PC399_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF2593556.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            5605..6495
                     /gene="metA"
                     /locus_tag="PC399_002707"
     CDS             5605..6495
                     /gene="metA"
                     /locus_tag="PC399_002707"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF2593557.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            6983..7426
                     /locus_tag="PC399_002708"
     CDS             6983..7426
                     /locus_tag="PC399_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2593558.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7457..9274
                     /gene="ftsH"
                     /locus_tag="PC399_002709"
     CDS             7457..9274
                     /gene="ftsH"
                     /locus_tag="PC399_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2593559.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9452..12154
                     /locus_tag="PC399_002710"
     CDS             9452..12154
                     /locus_tag="PC399_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF2593560.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            12188..12886
                     /locus_tag="PC399_002711"
     CDS             12188..12886
                     /locus_tag="PC399_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593561.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            13243..14199
                     /locus_tag="PC399_002712"
     CDS             13243..14199
                     /locus_tag="PC399_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF2593562.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            14376..14531
                     /locus_tag="PC399_002713"
     CDS             14376..14531
                     /locus_tag="PC399_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593563.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            14661..15674
                     /gene="aroF"
                     /locus_tag="PC399_002714"
     CDS             14661..15674
                     /gene="aroF"
                     /locus_tag="PC399_002714"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2593564.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            15696..16760
                     /locus_tag="PC399_002715"
     CDS             15696..16760
                     /locus_tag="PC399_002715"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF2593565.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            16760..18073
                     /gene="aroA"
                     /locus_tag="PC399_002716"
     CDS             16760..18073
                     /gene="aroA"
                     /locus_tag="PC399_002716"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF2593566.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELNKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            18051..19130
                     /gene="aroC"
                     /locus_tag="PC399_002717"
     CDS             18051..19130
                     /gene="aroC"
                     /locus_tag="PC399_002717"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF2593567.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            19133..20329
                     /gene="pheA"
                     /locus_tag="PC399_002718"
     CDS             19133..20329
                     /gene="pheA"
                     /locus_tag="PC399_002718"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF2593568.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRFIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            20376..21185
                     /gene="aroE"
                     /locus_tag="PC399_002719"
     CDS             20376..21185
                     /gene="aroE"
                     /locus_tag="PC399_002719"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2593569.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            21197..21730
                     /locus_tag="PC399_002720"
     CDS             21197..21730
                     /locus_tag="PC399_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF2593570.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDINMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            21751..22590
                     /locus_tag="PC399_002721"
     CDS             21751..22590
                     /locus_tag="PC399_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF2593571.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         9097 a         2447 c         4082 g         7099 t
ORIGIN      
        1 atagtataaa attattaata aaaacaaaag gagagatatt tatgttgagt ataggaacaa
       61 aagcaccaga atttacacta gaagataaag atggaaataa ggtaagtatg tcagatttta
      121 aaggcaaaaa agtagtagta tatttttatc ctaaagataa tacaccagga tgtacacgtc
      181 aagcttgtgc ttttagaaat gcatatgatg gatttaaaaa ggaagatata caagttattg
      241 ggataagtaa agatagtata aaatcacatc aaaaatttgc agaaaagcat gaacttcctt
      301 ttattttact ttctgaccct gacttagttg ctataaaagc ttttgatgta tggaaagaga
      361 aaaaaatgta tggaaaaact gcattgggag ttgtacgtgc gacatatatt atagatgaaa
      421 atggaattat tgaaaaagtc tttgaaaaag caaaaccaga cacaaatgca caagagattc
      481 ttgaatattt ggaaaaacag gagtaataag aatttatgat tataatgtta tcaccagcta
      541 aaaatatgaa gaatatagaa gtatttgata gagatttgag tttgccttgc tttatagata
      601 acacaaaaga aatagtggaa aatataaaga catttggtat agaagatttt aaaaataaaa
      661 tgaaaattaa tgaaaaatta gctgtattaa ataaaaatag atttgaaagc attaaatttg
      721 atagattagg aaaccctgca atcttaactt atgatggaat acaatataaa aatatagaag
      781 cagaaaattt tacaaggaaa gatgaagagt ttgcaaatag ctgtattcga atcataagtg
      841 gattgtatgg tgttgtaaaa ccatatgata gtatatatga atatagatta gaaatgcaga
      901 caaaattacg agtaggtgag tttaagaatt tatacgaata ttggggaaat cgtatatata
      961 aagaactaat caaagaaaaa acagctatcg ttaatctttc atcaaatgaa tacagcaaat
     1021 ccatagaaaa atttattaaa gatagtgata catatattac atgtactttt aaagtaaata
     1081 aaaatggcat attaaaagtg gaatctacac aagcaaagaa agctagaggt atgatgacaa
     1141 aatacattgt aaaaaatagg ataagagata ttgaagaact gaaaaagttt aatttagaag
     1201 gttataagta taaagaaaac ttatctaaca atagtgaata catatttgta aaagagtaga
     1261 agttttgtta aatattaaat ggcattgtta aatttaggta aaatatactt agtgtgtatt
     1321 gaattgaact tgattttaaa atataattat tcttaaatta aaaaaagaaa ggagtggtct
     1381 tccaaaatgg aaaatcacaa acagatgcct tggcattcaa gagcaacgca tgaaatttta
     1441 gaaagtctag attcatcaga ggaggggctc accgataccg aggcggaaaa acggcttaaa
     1501 gagaatggct taaatgaact tagaaaaaaa ccacctagaa caactttaca aatgctttgg
     1561 agtcaaatta cagatgctat ggttatgata ctaataggag cagcaatatt atctttgata
     1621 tttggcgaat ttacagaagc ttttgtaata ctaataattg taacagtaaa tgcagttatt
     1681 ggaattgttc aagagaaaaa agcagaatca tcattagaag cattaaaaaa tatgaactca
     1741 cctacagctc gtgttatgcg tgatggtgaa gaaaatgtta ttccagcaag taatcttgta
     1801 gtaggagata ttgttttttt agaagatgga gctatggttc cagcagacct tcgtttaata
     1861 gaaacatcaa gtttaaaaat tcaagaggct tctttaacag gagaatctgt tccatcagaa
     1921 aaagatgcag atacaatctt acctaaagaa tgtgtattag gagaccgtag caatatggcg
     1981 tatacatctt caatagtcac atatggtaga ggtgtaggtg ttgtagtagc aactggtatg
     2041 gatacagaag tgggtaacat agcacatctt ttagattcac aagatgattt tgatactcct
     2101 ataaaacgta aacttaatac tgttggaaag acattaagtg tcataggtat tattgtatgc
     2161 gttgttatat ttgcaatagg tgcattttat cagagacctc tcttgccaat gtttatgatt
     2221 gcaatttccc ttgccatatc aattattcca gagggattac ctgcaacagc tactattgta
     2281 atggctttag gcgtacagcg tatggctaaa agaaatgctt taattagaaa attacctgct
     2341 gttgaaacat taggaggagc tacagtaata tgttgtgata aaactggtac gttaacctta
     2401 aataaaatga ctgttacaca tattgctgtc aatggagatt ttgaatctgg aaaagtgaca
     2461 tcaatagata ctgcatctga aaaacatcca ttagtgtata aagagattgt ttatgctggt
     2521 gcactttgta atgatgcatg tattaatcca gataaaacag atgaaatatt aggtgaccca
     2581 acagagggcg cattaatttt tatggccaaa aaatttggaa tagatcaaga agctcttgaa
     2641 gaagaatatc cacgtgtatt tgaacagcct tttgattcag atagaaaatg tatgacaact
     2701 gttcataata tagatgaaaa gattattgct tatactaaag gagcagtaga cgaaattctt
     2761 gacctatgta ctaaaatatt aacttcaaaa ggtgaaagaa acattaccga agtggataaa
     2821 aagaatatac acgaattatg tcttaacatg tctaaagatg cattgcgtgt acttggtttt
     2881 gctaagcgtg agatttcatc tataccaaaa gaagatagtg aaaatataga atatgattta
     2941 acatttatag gtatggttgg tatgattgac ccaccaagaa ctgaggtaat tgatgcagta
     3001 agtacttgta aagaagcagg tataagaacc ataatgataa caggtgacca taaagttact
     3061 gccacaacaa tcgctcatga gttaggaata tggagtgaag aaaatactgt gatatctggt
     3121 gatgaacttg ataatttgag tgatgatgaa ctagatgaag ctgttaaaaa tactacagta
     3181 tttgctcgtg tatccccatt tgataaactt agaattatac aatctctaaa aagaattgga
     3241 gaagtaccag caatgactgg ggatggtgta aatgactctc ctgccttaaa gtctgctgat
     3301 attggtatag ccatgggtat aagtggtaca gatgttgcaa aagattcttc tgacatgatt
     3361 ttaatggacg atagttttac aacaattgct tatgcaataa aagaaggtag acgtgtatat
     3421 agaaacatac aaaaagttat acaatttttg cttgttggta atatagcaga gatacttacg
     3481 ttgtttgtag caatattatt aaattgggat actccgcttc ttgctgttca tattctatgg
     3541 gtaaaccttg caactgctac gcttccagcc cttgctcttg gagttgaccc accaagcaga
     3601 aatataatga agcaccagcc agtgaaatca accactttgt ttgaaaaaaa tcttatacaa
     3661 agagttgtta cccaaggtat atttgtggca ataatgacta ttttagcata taaaattggt
     3721 cttaatatag aaaatcattc tgtaggtcaa acaatggcat tttgtgtatt agcattttca
     3781 cagatgttac gagcatttag tcagcgttca aatacagatt caatgtttaa tagaaataat
     3841 ggtaaaaatc catttctttt aatctcattt ttaacttctg catttttaat agcagtaata
     3901 ctatttgcac ctgtttttag aaatgctttt aacttaacag ttttaaataa cttagagtgg
     3961 cttataacga ttgttttagc cataatgtct gtaattcaag tagaggttgg gaaaattatt
     4021 aaaagaaaaa aattaaaaaa atagttgaca aaattaggaa aaatatataa gatataaaac
     4081 atagaaaaat taaataaatc ttatccagag aggtggaggg actggcccta tgaaacccgg
     4141 caaccagtat atttatatac agtggtgcta aatcctgcgg cgaaagctga gagatgagta
     4201 taattaagct tatacagagc ctgagttata tactcaggct ttatttattt taactctcat
     4261 ctggtaaata gtttaatttt ttttactaaa atctaagggg gatacaagaa tgtataataa
     4321 agaaacaata tgtgtgcaag gaaattataa accaggtaat ggagaaccaa gagtactacc
     4381 tttatatcaa agtacaactt ttaaatatag cagtatagac caacttgctg aattatttga
     4441 tttaaaagtt gatggacata tatattcaag aataagcaat cctactattc aagcttttga
     4501 agaaaaaata agtttactag agggtggagt atcttctgta gctgtatcat cagggcaatc
     4561 tgcaaatatg ttggcagttt taaatatatg taaatcagga gatagtatac tttgttcttc
     4621 aaaagtatat ggaggaacat tcaatttact aggacctagt cttaaaaaat ttggtataga
     4681 tttaatatcg tttgacttag attcaagtga agatgagata gtagaacttg caaaggaaaa
     4741 tactaaggtt gtgtttgcag aaacacttgc aaatccaact cttgaagtca tagattttga
     4801 aaaaatagca aatgtagcta agagaattaa tgttccattt attgttgata attcattagc
     4861 atctccagtg ctttgtaacc ctttaaagta tggagcaaat atagttactc attctaccac
     4921 aaaatattta gatgggcatg cttcaagtgt tggaggaatt atagtggatg gtggaaactt
     4981 taactgggat aatggaaaat ttccagaatt agttgagcca gacccaacat atcatggtat
     5041 aagctatact caaaaatttg gaaatgccgc atatgcaact aaagcaagag ttcagttgct
     5101 tagagactat ggaaattgtt taagcccatt caatgcgtat cttactaatt taaatgttga
     5161 aacactacat cttagaatgg agagacatag tgaaaatgca cttaaaatag ctagattttt
     5221 agaaaaacat gaaaatgtag attggattaa ttacccagga cttgaagata acaagtatta
     5281 tgagaatgcc aaaaagtatt tatcaagagg atgtagtggt gttttatcat ttggagtaag
     5341 aggtgggtta gaaaatgcca aaaaatttgt ggaaaaatta cagatagcat ctttggttac
     5401 acatgtttca gatgtaagaa cttgtgttat acatccagct tcaactactc atagacaatt
     5461 aacagaggaa caattaattg catctggagt attgccttca ctaataagat tatctgttgg
     5521 aatagaaaat gtagaggatt taatagctga tttaaatcaa gctttaaatt tctaatattc
     5581 taagagaaag agaagtgaaa agatatggct ttaatactac caaagggatt acctgttatt
     5641 aataaattat tagatgaagg aatagatgta atttataaag aagattttaa aaaggaatta
     5701 aagtatgaag aaaacataga cactaagata gctatactaa atctaatgcc tataaagata
     5761 gatacagaat tagatttact aagaaggata gataaaacag gattcaacgt gtctgtggaa
     5821 tttattaaaa tttcaacaag agaaagtaaa agaagttgta atgaatatgt taaagaattt
     5881 tataaaacat ttgatgaagc caaaggagag tattttgatg gctttataat aacaggagct
     5941 ccagttgaac aaatggagtt tgaagaagtt gattattgga atgaactaga agaaattatg
     6001 gattattcta agagaaaaac taaatctaca ttatatatat gttgggcagc tcaagcaagt
     6061 ctttacaaat actacaatgt aaaaaaactt ccactaagtc aaaaatgttt tggagtattt
     6121 aagcataagg tagataaaga tagtaaaata gtagatggat ttgaaaatga attttttgct
     6181 ccacattcaa gacatactac tgtaaatatt gaagctttga aagagaataa agagttgagc
     6241 attgtgagtc attctaaaga agctggtccc tatataatta caaattcaag agatgtgttc
     6301 gtaatgggtc atagcgagta tgacaaatac actcttgata aagaatataa gagggatata
     6361 aataaaggag ataaaatatc tatacctcaa aattactata ttaatgatga cccttcagaa
     6421 gagccaactg taaaatggaa aaaacattca gaattgttgt ttagaaattg gataaaaaac
     6481 tatttgatac aataaatttt aagagtatat aaatgttaac taaaaaaata tagaaattta
     6541 taggtataaa gttaatctta atgatacttt ataccttatt tttttaaaat ttgaccttat
     6601 aatagtagtt ttatatttta agaaaatcac tttaggtgta tgtagataga ggtggcttta
     6661 attttatata tggtgcgata atgggagact aaacctttat agtatcaata aatttgtatt
     6721 ttaactttta aaagagatta aaaagggaga aatcagtgta aaatatatat agatttttta
     6781 ttaaatttta ttaaaaatat cctgtataaa attaaatatc aataagataa caaaatttca
     6841 attgaagtta caatattgta attgacaata aatacttaat actataatat ttaatacatt
     6901 aaacattata gtgttaagta tttattacag tatgcacttc agcctaaatt tgaaataaag
     6961 tattttcgta gggagcgttt gtatggatga gatgagtttt aagaaagagc tactagagtt
     7021 gactagagat ataaatatga aatttacaac tttattaagt gatttttatc agccacttgg
     7081 tattacagca gtgcaagctt tgattttatc tgagttatgt gaacatggag agaaaaaaat
     7141 aagcgattta gggaagaact taaacatgac aaatagtaat gtatcagtaa tctgtcaaag
     7201 gcttgaaaaa aatggctttt taaatcgcat aagagatata gaagaccaac gaattgtaaa
     7261 agtaaaagtt acaaataaat cattggatat acaagaacat atttcaagta gtatttttga
     7321 tagttatttt gaaaatatga ccagtgaaaa attgcaagat atggaagata taatagaagg
     7381 tttagagaaa ttaaataaac ttttaactta cattgattgc aaataacata aaaataattt
     7441 aaaggagaat aaaattatgg atgaacataa accccaaaaa aggtcagcac tctattatta
     7501 tacagttata ataatagtag taatgttttt aaatgcttta atatttccaa gtttaatgga
     7561 taaaaaaatg gttgagattg actatggaaa gtttctcaca atgttggata caggaaaggt
     7621 taaatctgtt gagatagaaa ataatagaat agcaataaat ccatctagta gcaaggataa
     7681 gaatatatac ataactggta gaatggatga cccagaattg gtagatagac taaaaaatgc
     7741 aaaagtagaa tttactaagg ttattcctaa ggaaaattca cctcttttaa gtattttact
     7801 tacatggata atacctatag gtattcttat gctatttggt aacttaatga tgaagtctat
     7861 gcaaaagcga atgggtggta atgctatgca atttggaaaa agcaacgcca aaatgtatgt
     7921 atctgctcaa agtggaaaaa gatttaatga tgtagctggt caagatgagg caaaagaagc
     7981 tttaactgaa atagtagatt tcttacacaa tccagaaaag tataaaaaga taggagcaca
     8041 aatgcctaaa ggagcacttt tagtaggacc tccaggtaca ggaaaaacac tccttgcaaa
     8101 agctgtagca ggtgaagcaa atgtaccatt tttctcaata tctggttctg agtttgttga
     8161 gatgtttgta ggaatgggag cttctaggat tcgtgattta tttaaacaag caaaagaaaa
     8221 agctccatgt atagtgttta tagatgagat tgatactatt ggtaaaaagc gtgataatgg
     8281 aagtggatta ggtggaaatg atgaacgtga acaaacttta aatcagttgt taagtgagat
     8341 ggatggtttt gatggtggaa ttggagtagt aatacttgct gctacaaata gacctgacag
     8401 tcttgacaaa gcattacttc gtccaggaag atttgataga cgtataccag ttgaacttcc
     8461 agaccttgga ggacgtgaag ctatattaaa ggtacatgct caaaatgtaa atgtaagctc
     8521 agatatagat tataatgcaa ttgcaaggtc tacatcagga gcatcaggtg cagaacttgc
     8581 aaatattgta aacgaagcag cacttttagc agttaagtct ggcagagata tagtagaaca
     8641 acaggatttt gatgaatctg ttgaaactgt aattgctggg tatcaacgta aaggagctgt
     8701 aatttcagat aaggaaaaga aaattatagc atatcatgaa ataggtcatg cacttgttgc
     8761 tgctatgcaa aaacattctg cacctgtcca taagattact ataataccta gaacttcagg
     8821 agcattggga tatactatgc aagttgctga agatgagagt gtacttatgt ctaaagaaga
     8881 agcaattgat aaaataacta cttttacagg tggtcgtgct gctgaagagg ttatctttaa
     8941 cacttgtaca tcaggagcat caaatgatat agaacaagct actaaaattg caagagctat
     9001 ggttactcgc cttggtatga gtgaagagtt tgatatggtc gctcttgaaa ctgtaagcaa
     9061 tcaatattta ggaggagata cttctcttgc ttgttcttcc gacactgctg ctaaaataga
     9121 tgaagaggta tttaaaatag taaaggcttg tcacaaaaat gcaattgaaa tcttagaaaa
     9181 taatattgat aaattgcatg aacttgctaa ttatttatta gaaaaagaaa ctattacagg
     9241 tgatgagttt atggaaatac ttaacttaaa ctaaaatgat tatacacaag ataaaaatgc
     9301 ctattgttag gcatttttat tttacaacta aattttttaa attcatattt aaatctttac
     9361 ataatcttaa tcattaaaaa tacatcttaa ctacaatttt atatcggaat tgatattata
     9421 atagtaatga atggtgtaag gaggtttttt aatggataga cctaatcctg atattctatt
     9481 agaaaaaatt aaaaatgaag aagaaaaact aagtagaggt caattaaaaa tattctttgg
     9541 ttatgcagct ggagtaggaa aaacctactc tatgttggaa tctgcacaaa atttaaaaaa
     9601 agtgggtgta gatgtagtag ttggatatat tgaaccacac actagaccag aaacattggc
     9661 tttacttgat ggcttggaaa ttcttcctgt aaaggagata gaatataaat ctataaaact
     9721 aaaagaattt gatttagatg ctgctttagc aagaaaacca gaagttatat tagtagatga
     9781 atttgcccat tctaatgttg ctgggttaag gcatacaaaa agatggcaag atatagaaga
     9841 attgctgtta gcaggcataa atgtctacac tactgtaaat gtacaacatt tagaaagtct
     9901 aaatgacatt gttgagataa ttacaaacgt atcagttaaa gagactatac cagataagtt
     9961 tttggataca aatacacagt tagaacttat agatgttgaa cctgatgtac ttcttgaaag
    10021 gttcaatgaa ggtaagatat ataagaaaga acaagctact agagcaaaaa ataatttttt
    10081 tataaaagat aacctggttg cacttagaga aatcgcactt agaaaaactg cagagagagt
    10141 taataaagaa gttcaaatga ccagactatc aaagggtgat gtaactgtaa tacctacatc
    10201 agatacactt cttgcatgta taagtccatc tccatcttca gcaaaagtta ttagaactgc
    10261 atccagaata tcagacagta catttgcaaa atggatagct ttatatgttg aaactccaaa
    10321 cacagcaaga cttccatttg atgaacaaaa acaactacaa aataacctaa aattagctaa
    10381 gaaattaggt ggagaaataa ttgttttaca tggtgaaaat ataatagagc aaatacttag
    10441 aattgcaaag ctgagaaatg taacaaaaat tgtaataggt agaaatcatt caagtaataa
    10501 aagatttagt aaaagattta aaaaagatat agttgacaaa ttaattgatg aagttgatta
    10561 tatagatata catataattc catataaatt tgcctcagat gttaaataca aacctaaaaa
    10621 ggataaatca tcaataaaat ctaaatttaa aattagcaaa gttgatttta taaaacttat
    10681 atttattact ttgatgataa gtattttagc atatgcagta caaagcatgg gatttataag
    10741 agaaaatata ttgttaatat atatgttggg tgtagtttta gtttctatgt ggactaaagg
    10801 gtatagtaca ggtataataa gttctgtatt taatatagtt cttttaaatt attttttcac
    10861 agcaccatta tatacattat ctatagcaga ttcaaactat atagtaacac tggttgtatt
    10921 ctcaatagta ggtataataa ctagcactct tacgtctaag atacagcatg aagcagaaac
    10981 tgctgctaaa agagaagaaa atacaaaaat gatatatcaa ataattaagg gatttttaag
    11041 attatcaaat aaagatgata tagtaaacaa aggtatagaa cttttgtctc ttagcttaag
    11101 tagagatgta atttgttatt taaatgtaga taagcataat aaatcaaagc tttacaaaaa
    11161 aaatactaat aataaggata aaaatgattt ggatagtgaa gatgaaaaag ctgttgcaag
    11221 ttgggtttta tccaattcaa cagtagcagg aaatgacacg gatacacttc ctggttcaaa
    11281 gggatactat ataccaattg taggaatgaa tttaacttta ggagtagtgg gaatatcatg
    11341 tattgattca aaattggata cagaagatat aagcttaatt gaaacaataa ttgctcaaat
    11401 ggcaatagcg cttgatagag aaatcttgtc agaagctaaa gaaaatacca atcttgagat
    11461 tgaaagggaa agattaagaa gtaatctttt aagagcagtt tcgcatgacc ttagaactcc
    11521 actagcagga atatctggtg cagtaagtac aattataaaa aacaagggta ctataggtca
    11581 agatataata gatgaattac ttagtggtgt atatgaagat actcagtggt taataagact
    11641 tgttgaaaat ttacttagta tgaccaagat agatgaaggg aaattggaag ttaaaaaaca
    11701 ttctgaactg gttgaggaaa ttatatcaga agcgttacaa aaaataaaaa aaagaattga
    11761 aaatgcactt atagatatta atataccaga acagatatta tttgttccta tggatgcaaa
    11821 attaatagaa caagttttaa ttaatttaat agataattct ttgaaatatt caaaggaaga
    11881 ttgtaagata gagataaatg tatatgagaa agatgattat gtttggtttg aagtatctga
    11941 taatggtcct ggtatatcaa aggaacttaa aaagcatatc tttgacagat tttttactgg
    12001 agaagaagga gctaaagatt ccagaaaggg tgttggactt ggattatcaa tttgtaaatc
    12061 tataattcaa gctcataaag gtgaaattat ggtagaaaac aataaagata agggttcaac
    12121 ttttaagttt agtttgccta aggaaaatga atagttttaa attttaaaat tagttttgga
    12181 ggaagaaatg agtaaaaaac ttatattgtt agtagaggat gataagacaa taagaaaatt
    12241 tatatctact gcacttttaa cacaggatta tgatgtcaag gaggctataa ctggaaaaga
    12301 aggtatatct atagctgtct catattctcc agatgttgtt cttttagatt taggcttaga
    12361 agatatggat ggaatagagg taataaaagc tatcagacaa ttttcaaata taccaataat
    12421 tgtagtttca gctagagagc aagatagaga caaggtagaa gtatttgatg caggggcaga
    12481 tgattactta acaaaaccat tctccatagt agagttatta gcaagagtaa gagtagcatt
    12541 tagacattca caagtagaag ctcaacaaaa agatgatgtt aaatctacgt ttgaagtaga
    12601 taaattattg attgattttg ataaaagaaa agttattgtt gatgatgttg aagtccattt
    12661 gacacctatt gaatataata ttctttcatt acttgcaaag tatcatggaa aagtgcttac
    12721 tcataatttt ataataaaag aaatatgggg tagtgttata gggaatgaga ctaagtcttt
    12781 aagggtcttt atggcaacgt taagacgaaa aatagaaaaa caacctgcaa acccaagata
    12841 tataattaca gaagttggtg taggatacag attaaatgat gaatagttgt aatatattaa
    12901 ttattagatt atgtgcttat taatatatat taaaattata tagtttagtt aaaataagaa
    12961 tgaagtgtat gtgcttgttg tcagtttagc acatgcattt tattttttag cttttttagt
    13021 atcaataatt ttttagtatc aataatatga aatatgtttt aaaataagta tatagtgtaa
    13081 tctgtaataa aaactttggt taaataataa atgtatttaa cacttacctt ataataaatt
    13141 taatataaat attaaataat aattattaaa atagtgtaaa ataattacag taaggattat
    13201 aaaaaatttt tagaatgtaa attgaaattt aggagtagat gtatgtatat actgaattta
    13261 aatacaagag agactattga agattttaga gataaatttt atgtagcaga aaattcttat
    13321 cttatattaa gtgctccaaa aaatttaaaa ttgcttaagg aaactttgga tatagatgaa
    13381 ataactttta atgattgtct taaatttgat gaaataacca aacttgattt atttgataat
    13441 tacgattttt taagcctaaa tacatttgaa ctaagagatg gagaagctgt tatagaagag
    13501 gtaaatatgt atttatcaga taattttata ttggttgtag tgaatgaaga acacttttta
    13561 tttgaatttg taaaaaatat tattttgaag aactctcaac ttgaaaaaaa tccagttatt
    13621 aatctattta agataaacta cttgattctt agagaagtta taaaaaatgg ttttgagtct
    13681 ttggaaaaag ttgaagagtt aattcttcaa atagaagatg aaatgatgga caacattaat
    13741 aagaatcatg tatctagaat aagtgatgta agaggattaa caagaattat agttaagaat
    13801 actagacctc ttttgtatat tggggataga atagttaagg aaaatataag atatttaaaa
    13861 tattcaaatg taaaaaaata taatttagaa aattttcaag gtatagattt tggaattgac
    13921 aaattataca atttcgcatt gtcaacacga gagttagcag ataaattatt agatatttat
    13981 tcttccagag taggtgagaa aacaaataac ttaattacaa aattaacctt gttaacagcc
    14041 atatcagcac cacttacaat tataactggt atatatggaa tgaactttag atatatgcca
    14101 gaattaaact ggatttatgg atatccagca actttatttt ttatgctatg tataattttt
    14161 gttggaatta taatatttaa aataaaaaaa ttattgtaaa ttacagatga tgtttaattg
    14221 attttataaa aatacatcaa ttgagaagct gatttaagta aataaaaaaa tattttaaat
    14281 tgaaaatatt tatagaaaat attgacaatt atattttctt tgttataatt attaaacaag
    14341 aagaataata aattataaaa atggaggata atactatgaa aggaatatta aaagaactaa
    14401 cagcaaaact ttatagcttt ttttacttta tgttctgggg cttttatttt agcgctggct
    14461 atttctttta caaaaatata tgtacttcct ttctggtgag gccttatttt agtgattttg
    14521 agcataagta aattttataa aaatgattat tttatatatt tatataagat ttagtgcttg
    14581 atagattatt ttagagaact cattagtgag ttctcttttt tattttaaat ataaaattat
    14641 aatattggga ggtagtaaaa atgatagtag tattaaaaat gggagcagat aaaaatgaag
    14701 ttaaaaaatt aattgaggct attgggagag aaggagtaga agttaatccc atagatggaa
    14761 cagaattaac tgttttaggt ttagttggag atactagtaa aatagatgcc aaaagaatag
    14821 aagcaaataa ggtagttgaa aaagttatgc atgtggtaga gccttttaaa aaggccaata
    14881 gaaaatttca tccagaacct tcaatcataa atgttaatgg aatggaaatt ggttcaaaga
    14941 aaattgctat gatagctggg ccttgttctg ttgaaacaga agaccaaata gtatctatag
    15001 ctaaagatgt gaaaaaatca ggtgcaggat ttttgagagg tggagcattc aaaccaagaa
    15061 catcaccata cgctttccaa ggattgaaat atgatggatt ggatttatta aaaaaagcaa
    15121 aagaaaaaac agggcttcct atagttacag aaataatgtc aacacaagat atagatatat
    15181 ttgaagaaaa tgtagatgta attcaagttg gagctagaaa catgcaaaac tttgatttat
    15241 taaaagaatt aggtaaaaca aataaaacaa tacttttaaa aagaggatta tctgctacaa
    15301 ttgaagagtg gctaatgtca gcagagtata taatggctgg aggaaatgaa aatgtagttc
    15361 tttgtgaaag aggtataaga acttttgaaa catatactag aaatactttg gatttaagtg
    15421 caattttagc tgtgaagaaa cttagtcatt taccagttat agttgaccca agtcatgcag
    15481 caggtaaatc atggatggtt gactctttat ctaaggcagc aattgcagtt ggagcagatg
    15541 gtctaataat cgaagttcat aatgacccag cacatgcact ttgtgatgga aaacaatcga
    15601 ttaagccaaa tgaatatgat gaattgataa gtgaattaaa gactatcgca tcagcagttg
    15661 gtagagaaat ataatctata aaaggatggt taaacgtgga agaaaaacta ttagtaaatt
    15721 taaaagaaaa tagctacaat atatttatta aaaaaaatat actaaagaat ataggagaag
    15781 aaataaaaaa aatatatttt ggaaataaga tttttattgt aactgatgaa aatatagcaa
    15841 aattatactt ggataaagta aaaaataatt taagtgaatc tggatttgaa attaaatctt
    15901 taatattaaa atctggtgaa gaaacaaaat catttaatac tcttcctaaa atatacgata
    15961 gtttacttga ctttaagtta actagaaaag atttgattat tactttagga ggaggggtaa
    16021 taggtgattt aggtggattt gcagcagcaa cttttttaag aggtataagt tttattcaaa
    16081 ttccaacatc tttattagca caagttgata gttctgtagg tggaaaagtt ggagttgacc
    16141 ttgagagagg caaaaactta gtgggaagct tttaccaacc aaaggctgtg tttatcgacc
    16201 caaatgtatt attatcactt ccagaaaaat tctatagaga tggaatggca gaagttataa
    16261 aatatggatg tataaaagac aaaacttttt tcaataaact tttaagttta aaaaatagag
    16321 aagaagtaat gcaacatata gaatacatta tacataaatg ttgctttatt aaaaaaactg
    16381 tagttgaagc tgatgaaaaa gactttggag atagaatgct tctaaacttt ggccatacat
    16441 taggtcatgc tattgagaaa tactacaatt ttacgggtta tacccatgga gaagctgttg
    16501 ccataggaat gtataagata actttagaaa gtgaaaagat aggaattaca aaaaaaggga
    16561 catcagaact tataaaatca attttaatga attataattt gccttatgag attgaattaa
    16621 gagataataa atctatagta gatgctatat ctcttgataa aaaaaatcta ggtgatacat
    16681 taaaaataat acttttaaaa gatattggag agagtattat atataatact acttctaaat
    16741 tttttcagga ggtaagataa tgggaagtct aaaaatatat ccaagtaaat tatctggaga
    16801 tgtaaaaata ccaccatcaa aaagtatggc acatagggca gttatttgtt catctctaag
    16861 caatggaaaa agtagaattt ctaatataga tttttctgat gatataatag ctactataag
    16921 agcaatgaca tcattaggag ctattataga aaagaaagaa gatattttag aaatttcggg
    16981 aattttttct aaagaaggta ttttaaatag agagaatcag ttgaatcaac ctaaattgac
    17041 aatagattgt aatgaatcag gctctacatt gagattttta gtacctatat ctttagcatt
    17101 tgatggtgta aaaaggttta taggtagagg aaatcttgga aaaagaccac ttgatactta
    17161 ttatgagata tttgacagac agaatataaa atatagttac aaagaaaatc agttagattt
    17221 gattatatca ggtaagctta aaccagatga atttagagta aaaggaaata taagttctca
    17281 gtttatcaca ggtttactgt ttatattgcc tacgctagag agtgattcaa agataataat
    17341 tactactgag ctagaatcta aaggatattt agatttaact ctttcaacga taaaagattt
    17401 tggggtagaa attataaaca ataactataa agaatttatt ataaaaggaa atcaaacgta
    17461 taaagcgaga gattataaag tagaaggaga ttattctcaa ggagcatttt atttatcagc
    17521 agatgctata ggagaagata ttagcatatt agaccttaaa gaagattctc ttcaagggga
    17581 tagtgaagta gttgaaattt tatctaggat gggaatggaa atattaagag aaggcaacaa
    17641 aataaaagga ataacaaatg gtttaaatgg tactttgata gatgcatctc aatgcccaga
    17701 tataatacct gtattatctg tagtagcatc cttaagtata gggaggacta caataattaa
    17761 tgctggtagg cttagaatta aagaatgtga tagacttcat gcaataaatg ttgagttaaa
    17821 taaacttgga gcaaatatag aggagaaaga ggatagtttg ataatcgaag gagtaagcaa
    17881 gttaaatggt ggagtagagg tgtggagtca caaagaccat agaattgcta tgacacttgc
    17941 aattgcttct tgtagatgtg ataaaccaat aatattaaaa gattttgagt gtgtatcaaa
    18001 atcttatcca cattttttta aagattttaa gatgttagga ggtcgaattg atgagtggaa
    18061 tatggggaaa taatttaaaa gtatcaattt ttggagaatc tcatggaaat gctataggta
    18121 taaatataga tggtcttcct tcggggattg aacttgacct tgataatatt gataaagaaa
    18181 tgaagagaag agcacctgga aaaaattcta tatctacttc aagaaatgaa agtgatatac
    18241 cagaaattct aagtggatat tttaatggaa gaactacagg aacgccatta tgtgcaatca
    18301 taagaaatag tgatactcgt tcaaaagatt atggagagct taaaaactta atgagaccag
    18361 gacatgcaga tttcacaggt aatgtgagat attctggatt taatgactat agaggtggag
    18421 gtcatttttc aggtaggata acagctccat tagtattttg tggggctata tgtaaacaaa
    18481 ttttatcaca aaaaggaata gagataggag cacacataaa aaaaattaaa aatatagaag
    18541 atatgagttt tgattatgta aatatttcaa aacaacagtt atcaaatttg caaactttgg
    18601 aactaccttt acttgattta tcaaaggaag aagccatgaa aaatacgatt atagatgcaa
    18661 aaaatcaagg agattcagtt ggtggtataa ttgagtgtgc agtagtaggt attaatgtag
    18721 ggcttggaaa tccatttttt gactctgttg aatctacttt atcacattta ttgttttctg
    18781 ttcctgctgt aaaaggtgta gagtttggtt taggttttga acttgcagat atgtatggct
    18841 ctcaaagtaa tgatgaaatg tattatgaag gaaatcaagt taagagcaaa acaaataaca
    18901 atggaggaat tataggaggg attactactg gaatgccaat aattttcaaa gtggcaatta
    18961 agccaactcc atcaattagc agacaacaaa acacagttaa tataaaagat aaaaaggacg
    19021 atattttata tattaaaggc agacatgacc cttgtatagt tcaaagggct atacctgtaa
    19081 tagaagcagt aacagccatt ggaatttttg atttgatgaa aggacgataa taatggagga
    19141 tttaacgaga tatagaaatg agattgacaa tatagataaa gagcttattc aattatttga
    19201 aaagagaatg aatacagttc ttgaaattgc cagacataaa atgaaaaata acactactat
    19261 acttcaaaaa gatagagaag aaaaagtact aagtagagcc gtagataatt tagataataa
    19321 agcatattca caagaaacgg tacagttttt taattctata atggaaatta gtagaggatt
    19381 acaaaaaaga tttatagata atgatggtga gcagaaaatc gagtttagtt tagattcatc
    19441 aaataagaat tttgatttgt caaatgtaaa taaatataag tctttaaaag atgaattaaa
    19501 taaaaaaaat atcctagtag gctttcctgg aaaatcaggt tcatttacag aagaagcact
    19561 aaataaattc ttcaacaaaa aaatatcaaa aaaacaattt aaagaatttg aagatgtttt
    19621 catagcactt aaaaataaag aaataaacta tggaataatc cccatagaaa attcatctac
    19681 aggagctatc tctgaaactt atgacctttt gagaaagtac ggtttttata tagttggaga
    19741 agaatgtata aaaatagacc aaaatttaat agggataaaa gacacaaagc ttgaagatat
    19801 aaaagaaata tattctcatc cacaaggtat aggacaatgt agtgaatttt taaaacaaaa
    19861 tagtacatgg aaattgatac cctttaataa cacagcaaca agtgcagaac ttgtaagaaa
    19921 acttcaagat aaaacaaaag cagcaattgc aagcaaaaaa gctgcaagta tttatggatt
    19981 agaaataata agtccttgta taaatgatat agcaaacaac tatacaagat ttgtagtaat
    20041 cagtaatcaa atacacattg aagaagaatc aaacaaaatg agtgtagtat tttctgttga
    20101 acatgaagca ggaaaacttt ataaagtact tggatacttt gctaaaaata atataaatat
    20161 gacaaaaata gaatcaagac ctatgaaaaa tgcctcatgg agatatttct tttatataga
    20221 ttttgagtgt tctatataca atgctaaagt gtacgattta ttagaattga tagaacataa
    20281 tacagcatat tttaaattta tgggagtata taaaaataaa actgaataga taagataaat
    20341 ttttatttaa tttataatta ttaggaggtg taagtatgaa ttttttcggt ctagtaggtg
    20401 aaaaactctc tcacagtgtt tcacctcaaa tccacaagag agtattcgag atattgaata
    20461 ttgaaagtgc ttataaaaac tttgaaattt caaaagaaga tatatctaag ctagacggtg
    20521 ctataaaatt attaggaata caaggggtaa atgttactgt accatataag gagcgtatta
    20581 tgaaatatct tgattttata tcaccagagg caaaacgtat aggagcagtt aatacaattt
    20641 tactcagaga aaatatgtta tatggttaca atacagatta ctttggtctt gatagtatgt
    20701 ttaaaatggc taatattgat gttcaaggaa aagtagcagt tatattagga actggaggtg
    20761 cttctaaagc tgctcttaca tattttattg attctggtat agaaaagctt tatgtttcaa
    20821 ctagaaaaaa ggatgataaa aaattattaa atagtaaagc tatattaata gattatgaag
    20881 aacttaaaca tataaaagga gatataatat taaatgctac accagtaggt atgtacccta
    20941 atgtaggaat ttcaccagtg tcaaaatcta ttatacaaaa ttttgatata ttaatagatt
    21001 taatttacaa tcctggagaa acagagtttt taagaattgg taatagtatg ggtaaaaaaa
    21061 cttgtgatgg attatatatg ctggtggggc aagcaataaa atctcaggaa atttggcaag
    21121 atactaaaat agataatagt attttagatg ttatatataa tgaactaaaa ttagaatttt
    21181 tgtaagggag attgttatga taaataaaac aaaagaaaaa ttgatattaa taggtatgcc
    21241 aggtagtgga aaaactacaa ttggtaaact tttggccaaa gaatataatt gtagtttctg
    21301 tgatatggat gattatataa ttcaaatatc tcaaaaaagt atagcagagt tgttttcaga
    21361 aggagaagat atctttagga actatgagac tcaagcatgt agagaacttt ctataagtga
    21421 taaaacagtg atatctacag gtggaggtgt aataaaaaag gatattaata tggaaatact
    21481 aaaagaaaca ggtataataa tttttattga tagacctata caaaagattt tggaagatat
    21541 aaatattaat tcaagaccat tattaaaaaa cggaaaagat agattgtata atctttacaa
    21601 tgagagaata aatctttata aaaaattttc agatatagag attttaaatg ataaatcatt
    21661 aaataatgct gtatataata ttacaaatgc agtcagtgaa aattttaaat ttgactttaa
    21721 agaaaaataa tctaggggga aataaaaata atgaatattg ttattgtagg actaggagtt
    21781 ataggaggct cttttgctaa agcattaaaa aaagctggat atgaaaatgt atttggagta
    21841 gatgtagatt tagaaacttt aaaaaaagca gaaaaagcaa aaatcataaa aaaaggatgt
    21901 actacaggta aagagttatt taaaaaagct gatttaatta tattatctat atatcctaga
    21961 ttagttgtag attttctaaa taataataaa aactttttta aaaaagggac tataattact
    22021 gatactacag gaataaaaga aacacttatc aatgatgtgt tgcaaattat tccagatgat
    22081 atagatttta tatttggaca tccaatggca ggtagggaaa aaaagggtat tgattttgca
    22141 agtgaacaag tttttaatgg cgcaaattat ataattactc caactggcag aaacaacata
    22201 aaaaatttag agttagttga aaatttaatt ttagagattg gatttaaaag agtaaaaaag
    22261 ttaacttcac aaaaacatga tgagattata gcttttactt ctcagttacc tcatgttatg
    22321 gcagttgctc ttataaatag tgatgaagaa ggtagagata cgggtaaatt tattggagat
    22381 agttatagag atttaactag aattgcaaat atgaatgaag acttatggag tgaacttttt
    22441 ttaggaaata gagataattt actaaaagtt atagaaaact ttgaatcaga agtaaattta
    22501 gtaaaagaag ctatatttaa caatgataaa aataaattaa tagagtattt taaaaaatca
    22561 tcaataagaa gagaagcttt agaaaaataa atattaatat ttaagctata tatagatggg
    22621 tataaatata agcgatagtg tatttaattg tattaataaa tatggattca atataattaa
    22681 atatgctatt gatatatttg tacccttaac tattgttatc tttta
//
LOCUS       DAMJWS010000071        22311 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMJWS010000071 DAMJWS010000000
VERSION     DAMJWS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.072"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            282..1343
                     /locus_tag="PC399_002722"
     CDS             282..1343
                     /locus_tag="PC399_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2593572.1"
                     /translation="MRDFLKNYKSSIILLACTIVGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKGTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKNLMVTQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     IGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIFMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1356..1553
                     /locus_tag="PC399_002723"
     CDS             1356..1553
                     /locus_tag="PC399_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2593573.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1717..2421)
                     /locus_tag="PC399_002724"
     CDS             complement(1717..2421)
                     /locus_tag="PC399_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2593574.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYTKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            2586..3311
                     /locus_tag="PC399_002725"
     CDS             2586..3311
                     /locus_tag="PC399_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2593575.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSMSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3371..4963
                     /gene="hcp"
                     /locus_tag="PC399_002726"
                     /gene_synonym="priS"
     CDS             3371..4963
                     /gene="hcp"
                     /locus_tag="PC399_002726"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF2593576.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDICGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5335..6240
                     /locus_tag="PC399_002727"
     CDS             5335..6240
                     /locus_tag="PC399_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2593577.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6364..7317
                     /gene="msrA"
                     /locus_tag="PC399_002728"
     CDS             6364..7317
                     /gene="msrA"
                     /locus_tag="PC399_002728"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF2593578.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTHIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            7470..8600
                     /locus_tag="PC399_002729"
     CDS             7470..8600
                     /locus_tag="PC399_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2593579.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8717..9685)
                     /locus_tag="PC399_002730"
     CDS             complement(8717..9685)
                     /locus_tag="PC399_002730"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF2593580.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            10020..10208
                     /locus_tag="PC399_002731"
     CDS             10020..10208
                     /locus_tag="PC399_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593581.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            10468..11667
                     /locus_tag="PC399_002732"
     CDS             10468..11667
                     /locus_tag="PC399_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF2593582.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIESDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11719..12222
                     /locus_tag="PC399_002733"
     CDS             11719..12222
                     /locus_tag="PC399_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2593583.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            12329..12646
                     /locus_tag="PC399_002734"
     CDS             12329..12646
                     /locus_tag="PC399_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593584.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            12804..13763
                     /locus_tag="PC399_002735"
     CDS             12804..13763
                     /locus_tag="PC399_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2593585.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            13878..14663
                     /locus_tag="PC399_002736"
     CDS             13878..14663
                     /locus_tag="PC399_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593586.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDKKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            14955..16274
                     /locus_tag="PC399_002737"
     CDS             14955..16274
                     /locus_tag="PC399_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2593587.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(16349..16777)
                     /locus_tag="PC399_002738"
     CDS             complement(16349..16777)
                     /locus_tag="PC399_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593588.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(16868..18244)
                     /gene="hydG"
                     /locus_tag="PC399_002739"
     CDS             complement(16868..18244)
                     /gene="hydG"
                     /locus_tag="PC399_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF2593589.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIREEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(18245..19330)
                     /gene="hydE"
                     /locus_tag="PC399_002740"
     CDS             complement(18245..19330)
                     /gene="hydE"
                     /locus_tag="PC399_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF2593590.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(19366..20574)
                     /gene="hydF"
                     /locus_tag="PC399_002741"
     CDS             complement(19366..20574)
                     /gene="hydF"
                     /locus_tag="PC399_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF2593591.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLIVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDSGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(20589..20840)
                     /locus_tag="PC399_002742"
     CDS             complement(20589..20840)
                     /locus_tag="PC399_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF2593592.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(21221..22261)
                     /locus_tag="PC399_002743"
     CDS             complement(21221..22261)
                     /locus_tag="PC399_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF2593593.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         8341 a         2681 c         3556 g         7733 t
ORIGIN      
        1 taatattata tatatttttt tatattgttt gttttattaa aattaatcta gaaaaaaagt
       61 tttaaaagca gaattttatt aatatttata tatagatatg aactattaat aaaacgttaa
      121 aaatacaaaa aaactataga aattgacata tattccagta tttgctaaag tagctgttgg
      181 ggaaaattac aaaaattatt aatgcgcatt agataattta ctttaatttt tttagcattt
      241 ggggtaattt ataatataaa gcattagaaa ggaaaatata aatgagggac tttttaaaaa
      301 attataagtc atcaattata cttttagcat gtactatagt aggggcgata gtaggattag
      361 tacttggtcc aaaagcttca atactaaaac catttggaga tttattcata aatgctctat
      421 taatgatagt agtaccatta gtttttctta gtgtttcttc aggtgttgct agtataaaaa
      481 atatggaacg atttggaaaa ataatgaagg gtacagctat agtatttatg ggaacaacac
      541 ttgtaattgc atttttaggt attacatctg cattattatt taatccgtct aaaggattag
      601 atgtatcaag tgtaaaaaat ttaatggtga ctcaagatgc agaaacttta gaatcaaaag
      661 gaacgttatt agaccaagta gtatcaagta ttacagttaa aaattttgga gatttatttt
      721 ccagtagtaa tttactacaa ttagttatat ttgcagtttt aattggaaca gcaatagcta
      781 tgatgggaga taggggaaat acaataagaa agcttttatc agaaggaacg gaagcaatat
      841 taaaagtagt aagtatattc atgctttatg ctcctatagg gctaggctgt tatacagcta
      901 caataattgg ggaacttgga agtaaaataa ttcaaggata tgtaagggtg tttgtaattt
      961 attcactaac agcagccatg tatttcttat tattttacag tctgtatgct tttttatctg
     1021 caggaaaaca agggtttaga caattctgga gaaatgctat aaaaccaata gcaacggcac
     1081 tagctacttc ttcaagttca gcatgtatac catcaaattt agaagctagt aaaaatatag
     1141 gagttcctga tgatatatca gaaacagtta tacctcttgg aataaatata cataaagatg
     1201 ggtcagtttt agcagtaata ataaaagtgg catttttatc aggtatttta ggtagtggaa
     1261 tggcaaatcc agcaacgata ggtcaattat ttataactgt atttgttgtt ggagtattta
     1321 tggctcctgt agcaggtggc tgacttattg gtgagatgct cgtggttagc atcttcggtt
     1381 ttccagtgga ggcaattcca ctgttaacag ttataactac tataactgat attcctaata
     1441 ctatattaaa tacaactgga aatacagtat catcaatgtt agtttcaaga ttagtagaag
     1501 gaaaagattg gcttatagat aaaacagcaa ttactacaaa gaaaataagc taattgtaca
     1561 cttaattaat aaagttaata ggtatcttaa ttttaataaa ttagatacct attaactttt
     1621 taagtaaaaa tataaattta agattcaaat atgatattta taatttgaat caactaatat
     1681 agtatatatt gaaataaata tgtttaaata tctattttat tttttaaaat aaaagatttc
     1741 aaatttattt ttgttaaaag ataacaagcc ttcatctcgc atcttgccaa gctctcttga
     1801 catagaactt ctgtcaacac ataaaaaatc agccatttca tttcttgaaa agggaattgt
     1861 aaactggtgg ctacctgttt gttctatctg ttttgaaaaa tacataagca atttctcgcg
     1921 agttgtttta cgagaaagta attctatttt attgtgaagt aaaatgtttt tttcagctat
     1981 taattgtagc atattttcaa caagcctatt atgaaataca catgcagatg aacaaatacc
     2041 agttacactt ttaaacttta tgaataaaac ttcgcaagaa gatgttgtgg ttactgtaac
     2101 attactttta tgtagtctag tacatgcgaa tacctctcca aataaatctc caggcaagag
     2161 ttcagctaca atattcctat tcccttcgat atcttccttt attatatgag cacttccttt
     2221 aagcataatt ccaatagaag taacttcact tccaacagag aatattatac tatcttttgt
     2281 gtaagtctta tctacaacac ctaaacattc caacatttct tcaagttcat cacttttaat
     2341 tccttcaaat agaggaattt ttttaagtag gtctatatgc ttttctaaaa attttttcaa
     2401 ataatcactt ccttgttgca tatgcaaccg ataatttgtt ttcattgtac tatactaagt
     2461 ttataaaagc aaggggatat aaaaatccta ctacaagaaa tttgaaataa agaaatatag
     2521 gtattaaaaa tatatatata agaaatcaat actttaaatt aaaaaaattt tatgaggtga
     2581 tataaatgat gagaaaaata attaaaattg atgaggaaaa gtgtaatggt tgtggactat
     2641 gtgttgaagc ttgtcatgaa gaagctatag gaatggtaaa tggaaaagca aaacttttaa
     2701 gagatgacta ttgtgatggg ctgggagatt gtctgccaac ttgtccaaca aatgcgattt
     2761 catttgagta tagagaagca gcagaatatg atgaagctgc agtaaaagca aatatggaag
     2821 caaagaaagc acaaaagaaa actttagcat gtggatgtcc aggttctcag tctaagtcaa
     2881 taaatagaga ggtttcaaat agtatgtcta taagcaatga tatagtagag ataaaaggtt
     2941 ctcaacttaa tcaatggcca gtacaaataa aattagtacc aactaatgca tcatatttaa
     3001 agaatgcaag tcttttaata gcagcagact gtacagcgta tgcatatgga aattttcata
     3061 ataaatttat gaaaaataag gttacattaa tcggatgccc taaacttgat gaagttgact
     3121 atgctgaaaa acttacagag attttaaaag aaaatgatat taaaaatata gtagtaacac
     3181 gtatggaagt tccttgctgt ggtggtatag taaatgctgt taagacagct ctacaaaata
     3241 gtggaaaaat gataccatgg caaatagtta ctatatctgt agatggaaaa attgtagatg
     3301 gagaaattta attaagtgaa ttatagaaaa atataataaa ttttaataaa taaaaaggga
     3361 ggaataaaaa atggaaaaaa tgttttgttt tcaatgtgaa caaacagcag gaggtaaagg
     3421 ttgtactata aatggtgtct gtggtaaaaa ggggggtaca gctaacttac aagacgaact
     3481 tacaggagtg ttgattggtc tttcaagagc aactattgga aataagaata gaccaacaag
     3541 tgaaacagat aaaattatga tagaggggct attcactact ataacgaatg taagttttga
     3601 tgatgaagct ataaaaagac aaattgaaaa aactgaggta gaaaaaagta agcttgtacc
     3661 tagatgttca gattgttcaa caacttgtaa tagaaatgat aactatgata tgaaggattt
     3721 atggaatgat aatgaggaca tacgttcatt aaaatcatta atcttatttg gattaagagg
     3781 tatggctgcc tatgcatatc atgcaatggt acttggttat acagacaaag acgtaaatga
     3841 atttttctat gaaggattaa tagcagttgg aggagacttg tctattgacg aacttttagg
     3901 tctagttatg aaaactggag aaataaactt aaaatgtatg gaattgcttg atagagcaaa
     3961 tactgaaact tatggaacac cagaaccgac tcaggtatct atgaaaatcg agaaagggcc
     4021 atttattgta gttacaggac atgaccttta tgatttacac aaattattag aacaaacgaa
     4081 agataagggt ataaatatat atactcatgg tgagttatta ccagcacatg catatccaaa
     4141 actaaaagaa tataaacatc taaaaggtaa ttttggtaca gcatggcaaa accagcaaaa
     4201 agaatttgat aatattcctg ctccaatatt atttacaaca aactgtttaa tgcctgttaa
     4261 agatagttat agagatagag tatttactac agaagtagtt gcttatccag gtatggttca
     4321 tattgatgaa gataaagatt tttctagtgt aatagaaaaa gctcttgaac ttggaggata
     4381 tagagaagat gttcatatga ctggtattaa tggaggagat acattaacta ctggttttgc
     4441 aagaggtaca gtttcatctg tacaagataa agtagtagat gctgtaaaga gtggagctat
     4501 aagacacttt ttattggttg gaggttgtga tggtgcaaaa cctggaagaa attattacac
     4561 tgaatttgta aaacaagctc ctaaagacac tgttatattg acacttgcat gtggaaaata
     4621 tagatttaat gatttagacc ttggggatat atgtggctta cctcgtttaa tggatatggg
     4681 acaatgtaat gatgcttata gtgcaattaa agtggcagtt tcattggcaa atgcttttga
     4741 atgtggagta aatgatttgc ctcttagcat ggtactgtca tggtatgagc agaaagctgt
     4801 ttgtatactt cttacactac ttcatttagg tatcaaaaat atctatatag ggccttcttt
     4861 gccagcattc ttatctaaga atgtattaga tatattggtt gataaatttg cattgacacc
     4921 tatatctact cctgaagaag atatgaagaa gattttagga taaataaaca tatctatact
     4981 taaagatatt tatagttaaa atatttttaa gtatgccaca attaagataa ggggttaaac
     5041 ttaaaatttg attaaaaaat caattttgag atgacccttt atttattttt tgtaataatt
     5101 gaaaaataaa cctgctttca gtatattctt tttaagaatc tttgtattaa atttataata
     5161 cttagatata taatatctta tatattttat tttaagtatt aaaggtttga atgtaactta
     5221 aatattaaaa gaggaacatt gtaatatgta acttgcataa aatatatata ctatagtgaa
     5281 attattttga ttaaaattta aaatataagg tgttttatgt gggggtatat gtatatgaaa
     5341 aattctaatt ttaatatatt ggcatttgat ggtggtggat taaaaggggc attgagtata
     5401 tccatacttg agaggatagt aaaagagtat ccaaatctat taaacaatat aaatatgttt
     5461 ggaggtacat ctacagggag tctaattgca ctagggcttg cgtatggtgt aagccctaaa
     5521 gagataaaag aactttactc aattgaaaat tcaaagtata tttttaataa aagttatgca
     5581 gaaatactca gaccaaagta tgaaaacaaa aacttaaaag aagttttatt aagtatattt
     5641 ccagaggaac tagaattaaa agatttaaat aaattagtaa tgataccaag tttttatata
     5701 ggaaatgagg aaaatgcatg gaaaccagtg ttttataaca atatgccaaa ttcttttaca
     5761 aagactagca aagttgtaga tgtagcaatg gcaagtagtg ctgctccggt attttttcca
     5821 acatataatc gtcatgttga tggaggaata atagcaactg acccgagttt agcgtgtata
     5881 attcatgcca ttgacagtgg atttaaactt aagaatacaa gacttttttc aatagggact
     5941 ggatatgtat ataacagtat aaaggcagat actactgaat ggggagctat tgactggata
     6001 attaataaag aacccgattt accaattata tctattacct tagaaggaaa ttctcaaatg
     6061 agtcagttat tttctcaaaa acttcttgga gataattact atagattaaa tcctaagatg
     6121 gaaaaagatg tagctatgga tgattgtgat gcattagaat atctaatgtc tttaggagaa
     6181 aattgtgata taaaagatgc ttttggatgg ataaagaaaa agagctttta tagaaattag
     6241 attcaatttg caattgtatt tttacacatg atttcattga gtaggtaaaa ataataaaat
     6301 gcagattatt aagttacttc ttagggaata aataaagtat aatttattta aggagtgatg
     6361 aaaatgataa aacttgctac ttttgcaggt ggatgttttt ggtgtatggt aaaacctttt
     6421 gataagtatg atggagttat taaagtagtt tctggatata caggtggtca tgttgaaaac
     6481 cctacatatc aagaggtatg ctcaggaact actggtcatt tagaagcaat tcaaataaca
     6541 tatgatgatg atataataag ttatgatgaa cttctaaata tcttctggaa acagattaat
     6601 cctacagatg atggtggaca atttggtgat aggggaactc aatataagac tgctatattt
     6661 tatcatgatg aagaacaaaa aaatatagct ttagagtcaa gagaacatct ggaaaatagt
     6721 aaaatatttg atagtaaaat agtgactgat ataattccag caactaaatt ctacgaagca
     6781 gaagaaaatc atcaggatta ttataagaaa agaccattaa actacaatat gtattataaa
     6841 gggtctggaa gatataattt tgtaaaaagc cattgggatg gaaacaatat aaatagggaa
     6901 gagttaaaaa agactttaac tcatatacaa tttgaagtca ctcaaaatga tatgacagaa
     6961 gtaccttttg aaaatgaata ttatgacaat aacaaagagg gaatttatgt tgatattgta
     7021 agtggagaag tgttattttc ttcaaaagat aaatttgatt ctggatgtgg gtggccaagt
     7081 ttttcaaagc cagttataga taaatctatc attgagagaa gtgatttctc acaaggtatg
     7141 tacagagttg aagtaagaag tgctaaagga aattctcatt taggtcatgt atttgatgat
     7201 ggtccaaaag aacttggagg tttaagatat tgtataaatt ctgcttctct taaatttata
     7261 ccaagagaaa atatggaagc tttaggatat ggagaatatt taaaactttt tgagtaatta
     7321 ggaatattta ttataaactt atctaaaagg tgaaaatata aaaaaatatt ttcacctttt
     7381 gtttttttgt caataatatg ataaattatt aattgtaatc ttatttatac caatagatta
     7441 agaatttaat tttgaaagag agaaaacaaa tgaatttaaa agactttaat ggaaagagat
     7501 taaaatcagc tagaattttt agagcaaaga cgatagaaca gttgtctaaa gaaactaaaa
     7561 taaataaaaa agatttaaaa gcttttgagg aaaataagta tgtaccaaat atagaaaata
     7621 cattaaaatt atctaatata ttaaattttc caaaagagta tttttataaa aatgaaaata
     7681 taaatatcgt tgtagaggat agccacttta accctcaatc taaactacct agagtagaag
     7741 agatttctta tagagaaaaa ataattataa tacataaaat atattcattt atggagaatt
     7801 atattaagtt tccaaaagaa aacttgccaa atagaaaagt aatgtcagat attaatataa
     7861 atgatataga atctcttgca tataagacta gagagttttg ggatttaaat agtactccaa
     7921 tagtaaatat ggtaagtttg ttagagtcta aagggattat aatatcaggt atgaatgtag
     7981 atagaaaagg ggcaactatc tttactcaaa aacagagaat tagtaaagaa tctaaatatc
     8041 taatatctct gggtaatgac aaaaagtcag catcaattag aaattatact ttggcatgtg
     8101 agcttggata tataatatca agtgaattaa gaataccaag taagcaattt tcagaagatg
     8161 agtatgcctg tgcattttta ttgccaaaag agagtttttt aaaagatttg actcatccag
     8221 aagatttaga ttattatgta gaactaaaga aaaaatggat tgtacctata tctgctatga
     8281 tttttagagc atataatcta gaaaagataa attacaaaaa atacaattat cttatgaatg
     8341 aaatggataa aaaaggctgg ttgatagaag aaccactaga caaaatgaaa ggttcaagcc
     8401 caacgtattt aaaaagagca gttgaattat tgatagaaaa taaaataatg agtgtaaata
     8461 gtatagtatc aagtttagag gaatttggaa taaatcttta tccagaagac ttagaattat
     8521 tgatgggatt gaaaaaagga ttgttatctc aagaggtaaa taaaaaaagt aaagttataa
     8581 aatttgatgg taaaaaatag gtttatattt tagtattaaa attaaaagca ggtacaaatg
     8641 atttaaacat taacttagtt aaaagtcttt tcaataagtc atttacctgc ttttagtgta
     8701 agaaagaata ttgaatctaa agtgtttttg aaagtgcaat atattccctt aaaacagaac
     8761 aagatttatt aaattcgttt tgctcatatt cagtcatatt gatttcaata atttcttcaa
     8821 taccatcttt tcctataaca catggaacac ctgcaaatac atttctttcg ttatattgac
     8881 cttctagtaa tgtagaaact ggaagaactt ttttctcatt gtgtagtata gcttttacaa
     8941 tttctactaa agcagtacct ataccaaatt ctgtacaatc tttaccattt attatactta
     9001 ttcctgtttt tttatttttt tcaactattg atggtaagtc taaattgcta taagtacttg
     9061 ggttgtcttt tatcatgtct aagattggtt taccaccaat tgaaacatga gaccaaggaa
     9121 ccatttgaga atctccatgt tcacccatag aataagcttg aattgattgt tgagcgattc
     9181 cagtttcttc agataaaatc cttctaagtc ttgttgaatc taaagctgtt ccagttccaa
     9241 tgactttgtt tttaggaaag ccagattttt cccaaatata atttgtaact acatcaacag
     9301 gattagatat gactacaaat ataccatcaa agccagaatc aactatgggt tttactatag
     9361 attttatcat tccaatagtt ggtcttagta aatctaatct agttgtgttt tgtctacgta
     9421 aaggcccttc tgaagagtct gcaacactta tcactactat gtcagaatct ttacagtcag
     9481 aaaaagttcc tttttctata tgtactttat gtggcagata tgaaactgca tcagctaaat
     9541 ctaaagcttg agcttttgat ttagattcat ctatatcaat cataaagagt tcatcacaaa
     9601 ctccttgagt tattaaacta aatccacaat gagaaccaac atgccctgaa cctacaatag
     9661 atatttttct tggctttatt gccattttaa atcactcccc ctaaaataac gatacattga
     9721 aaattataga tgttattaaa agctgttttt ggatatttta atattctatt atttcagaaa
     9781 atttatttaa tatgatttta tactaaaatt tttaaaaaag caatgttaat atactctcta
     9841 ttagtgaata ttaaagtatt atatgtatat taataattat taaaatatgt aatgtcactg
     9901 atagttagaa tatttaagtg ctaaaagatg tgtataaata ataatgatgt cagtttataa
     9961 tgatattaaa ttgagatata ctgtttctct aaattaaaaa ataaaggagt aaatgtgtta
    10021 tgaattatca tacaaaagaa gaattggtgg aagcactgag agtagtatct tcaagtatta
    10081 ttaattgtgg aaaaggacag aagaaatttt ctgaggagac atctcatcat acacgtttta
    10141 agaatattat agaagtaatg tatatttcaa aatcattaat aatggatgaa attagtaaaa
    10201 gagattgaag cttattttgc aatttaacat gttttaatat acattttatt atataagaaa
    10261 ataagtaaac atacatgtta taaaatattg gaataatata caattataaa tttgttaaca
    10321 ctaatatcta tatgttaaaa tatagtagaa gatgttagta aataatagga tgagttctat
    10381 tttaatacaa ttaaattaag cttagtttga aatttgtatc taaaaagtaa agtaatctag
    10441 aattaagaaa atggagatag aattataatg gaaaagttat attatacaaa tcagtatata
    10501 agagatttta cagcagaaat aattgaaata aaagaagtag aaaataagta tcatgtttta
    10561 ttagataaga cagctttttt tcctggtgga ggaggtcagt ttggagacct gggaaaaata
    10621 gagagtgatg atgtaataaa tgtgtatgaa gatggaaaaa aaatatacca tgtattagat
    10681 aaaaaaccta taaagataca taaggtaaag tgtagtattg attgggatag aagagaagat
    10741 ggaatgcatc aacattttgg acaacatgta ttgtctggat gtttttttaa aacttttaat
    10801 gcaaatacga caggttttca tttgggaaat gagtatagta cagtagatat agaaggtaat
    10861 ttagaagagg ataaaattag agagatagag ttgtttgcca atgaaattat tcgtcaaaat
    10921 atacaagtag agacattact tccaacgaaa aaagaactaa aaaagatttg gttaagacgt
    10981 gatttgccaa atactgatga agagataaga gtaataaaaa taggtgattt agatacgaat
    11041 gcatgttgtg gagtgcatcc aaaatcaact ggtgaattga gaatgataaa aataaagaaa
    11101 tgggaaaaac ataagtcaag tacaagaata gagtttctag caggtaaaag ggcagtggat
    11161 gaagtattga agagggatat atatttgact aaaatatgta gatatttaag ctgtagtgag
    11221 gaagatgcta taaatggtat tgtaaatcta aatagtaaag tggaagaggc tttaattaaa
    11281 aaaagaaggc ttgaagaagt agtatctaca tatcaggtca aagaaatgat tgaaaaagca
    11341 aataaggttg gcaatatttc ggtcattaaa aaaatttatg atgatgaaga tttgaaatat
    11401 gtaaataaga tagcaaataa aattgcagaa tatgaaaata gtattgcatt attagctgtt
    11461 aaatttaatg ataaaataaa tttagttttt gcatgttcta aaaatttaaa agatattaat
    11521 atgggagttt tattaaagga tgcaataagt cttatagatg gcaagggtgg tggaagtaaa
    11581 gtattagctc aaggaggagg taagaataat ggaaatttag aggctacact tgattatgca
    11641 tttatgaaga tagaaaaaac tctataaata tattatataa aaaaccttag tagataaaat
    11701 agatatggag gaataagtat ggagcaacaa aaggactaca tagagttata tgaaaatgaa
    11761 gaactaatag agataagaga agcacgtatg gatgatttgg atacaatagc aaaatttaat
    11821 tataatttgg ctaaagaaac tgaaggaaaa gaacttgata tggatgtttt aactaaaggt
    11881 gtaaaagcat tgttattaga tgagagaaaa ggaaaatatc atgtatatac agtatttgat
    11941 aaagttgttg cacagattat gtatacatat gagtggagtg attggagaaa tggaaatttc
    12001 ctttggattc agagtgtata tgtagataag gaatatagaa gaaaaggtat ctttaattat
    12061 ttatttaatt atataaaaaa tatatgtgat aaagatgaaa atatagttgg aatgagatta
    12121 tatgtagaaa aagaaaacat aaatgcaaaa gcaacatatg agtctttaaa tatgtatgag
    12181 tgtgattata atatgtatga atacgaagta atacattcgt aatataaatt tgtaatactg
    12241 ttatactgat gttataaagt attgattata ttgacgatta tatgaaataa actggatatt
    12301 ataattagaa tttttagggg gtataaaaat gggcgtttta aaaggaatgt tttatgtatt
    12361 tttattttta gacttagtgt caatattctg tttctttttc aataaaggaa aaatagcatc
    12421 taataaaatt gtgtttaatg caataggagt acttacattt gtactttgtt ttatgctttt
    12481 tagttattat cctaataata atcttattgg aaaatttatt gcttctctat tttttatatt
    12541 tggagttgca ggagttatgt tgaaagaaaa aaatttttta tatgctagac ttcttttaac
    12601 tgtagttata gttttttcta cattaagatt gttcgttatt caataaaatg tatttgtatg
    12661 aaaatttatt aaagtctaca catttaagtg taggcttttt tgattttttg tttgaaaatt
    12721 aaggaaattt taaggaaatt ttaaaatcgt cttaaatatt tattgatata cttaaaatat
    12781 taatagcgaa aggagactta tcagtgatag aaagtaaaaa gtcagaaaaa ttgttactgt
    12841 caattatata cataatattt ttggtatata ttttaatgat tataagaata attttattta
    12901 gagatgtacc aatttacgct attttcaagg gaacgtttag gaatgtaaat ttaattccat
    12961 tttatacaat atatcaattt atagtagata gtaatcttga ctttatgaaa gctactgtaa
    13021 atattataga aaatataggt atatttattc ctatgggaat ttttttacct atagtatgta
    13081 aaaatctaaa taaaaaaact ataattatag taattatact tgttagccta gcatttgaat
    13141 taacacaata tatttttgct cttggaagta gtgatataga tgatgttatt ttaaattcat
    13201 taggtggcat aattggaatt aatatttaca taaacatgaa taaattattt cccaatgata
    13261 ttaacagatt taaggctata ataggtacta gtttagtttt aggagttata ggattaggag
    13321 ttatttctaa aaattatcat aacttactaa cttttaaatt tagacctgat agaaaaatat
    13381 caaaaatatt aattgaagaa aatagggaaa taattaaaga tataaataaa gatacagtag
    13441 atatagttgg gacatttgag agctttaaaa aaggaataat aactattaaa gcaggttcta
    13501 ataacaaagt aaagagacca gagaatttaa ttgattctga tggaaatata agaatttatt
    13561 taaatgaaaa tacaaaatta gtatcttata taattaccaa agaatcaaaa atggatatag
    13621 ttaaatatga ggaatttgat gtgaagaatt tagatttatt aaggagatat gatactatta
    13681 atgtttggat tgataaagaa aatcagccaa aggataaaca tggtattatg gctagtaaat
    13741 tattaattgg attgtatgaa taaaatatta aacaagaata aaagaataac aaatataaaa
    13801 aacgaaaaaa ctatttctat aattattttt acgttgtgca taaaataaga acattagtga
    13861 aggagtagag ataaattatg gagataataa aatatatctt gtactttttt atatattcat
    13921 ttttaggatg gacagtagaa agtataggat gttctatagc aagcaaaaga ataatcaaca
    13981 gaggtttttt aaatggtcca atatgccctg tttatggttt tggagcggta attgtgatat
    14041 cactattggg aagatttaat aatgtagtaa tagtatttct attaggaatg attttaacta
    14101 cgatactaga atattttaca ggatttattt tagaaacctt atttcatgct aaatggtggg
    14161 attattctga caaaaaattc aatataaagg gtagagtatg tttaaaaaat gcaatatact
    14221 ttggtgtaat gtctgtttta ataataagat ttatacatcc atttataaaa tattttgtaa
    14281 gtataattcc atatagaata ttaatttcaa tggctataat tacaacactt tggactatac
    14341 ttgatttgat tgttactatt ataacactta aaaaattaga tataaagtta aacttattag
    14401 acgatataat tacagacctt aatgatataa atgtgaaatt ggataaattt gatagaggag
    14461 aaatacaagc tttatttaaa acaataaata acgacgaatt agaagttaga gaaaaaataa
    14521 acaaaataaa tagtaagttg gaccgtatag agtctaatat tattttacaa aaaagagtta
    14581 taaaagcttt tccacatata aaacataaaa aacaacaaga gcaactagaa cattttaaaa
    14641 aaattattaa tgagaaaagg taattataca atttaatgaa gacgaataga ataatggtat
    14701 ttataagatg attttcagag gactctataa aaatagaata gagtccttta ttttttgaaa
    14761 aataatgttt atttgataaa aataaaatat attgtttctg aataacaaat attttgaaaa
    14821 ttataaaaac atatattgac aatttgtgtc aaaaatacta acattaagta tataaagaat
    14881 tttatatgag aatatcttta taaatcgcaa taattttaaa acataaattt atttaaagag
    14941 gggatgattt gccaatgagc gagataacta gtcaaatgat aagtacagaa gaaaaaaaat
    15001 atgttgctaa aactcaaaag ataccttatt atccagtagc atttaaatca ggtgatggtg
    15061 caatgctata tgattatgaa ggtaatgaat atgtagattt tttagcaagt gcaggctctg
    15121 caaatgtagg tcatggtaat aaggaaatct cacaagcagt taaagaacaa atggatgata
    15181 taacacaata tacacttgca tatttccata gtgatcctcc tgtaaaatta gctgagaagc
    15241 ttgtggaaat agcccctgga gataatgata aaaaagtact atacagtgca actggttctg
    15301 cttgtataga tgctgctata aaattagcaa gagggtatac tggaagaaca aagataatat
    15361 ctatgtgtga atcttatcat ggaagtactt atggagctat atctatatct gctttaagta
    15421 caaatatgag aagaaaaatg ggtccattgt taccagaagt ttatcacttc cattatcctg
    15481 ataaaaatag aacagctaaa gaatgtttag atgagataga atatgctttt gcacactatc
    15541 taccagcaga agaggtagct gcaatattta tagagccaat tgcaggagat gcaggaataa
    15601 tagtgcctcc agtagagtgg gtacaaggtt taagtaaaat atgtaaagaa aatggaatat
    15661 tacttgtaag tgatgaaata caacaaggta tgggaagaac aggtaagtgg tttggtatag
    15721 aaaattttgg tgttgaagca gaccttatag ttcttggaaa atctgttgga ggtggattac
    15781 ctctaggagc agttgttgga agaacagaaa taatgcaaag tcttgatgca ccagcacatt
    15841 tatttacttt agctggaaac acaacagtat gtgtggctgc tttaaaatct atagagataa
    15901 ttgaaaaaga aaacttactt caaaagagta tagaaatggg agattatatt aaagcaggat
    15961 ttgaaaaact aaaagaaaaa tacgatataa ttggagaaat aagaggcata ggattatcta
    16021 taggagttga tatagttaaa ggaaaaggtt ctaatgaaaa acatccagat gctacagcta
    16081 agatatgtta tagatgtata caaactggat taataatgat attcttagga caatctactt
    16141 taagagtgca accacctcta gtaataacta aggagcaagt agataaagca atgaatatca
    16201 tagattctgc aatagatgat tatttaaatg gtagaattgg tgatgaagta tatgaagtaa
    16261 ctcaaggttg gtaaaataaa tattaaatga aaataggagt tatctcaatt ttagaaaatt
    16321 atttctgaaa agagataact cttttaagtt acccacaaat aagaaaattg tttgattcat
    16381 taaagactat atttcggatg tcgtttttgt gagtatcaga gacagtatta cgagaataat
    16441 tacctttatt cgtactatct gttaatttag ttataattaa gatagctaaa agaataagaa
    16501 ttactgtgta taaagcaata acatatggtt ttcttttata tttgtatttg ttattgaaaa
    16561 aagaatggcc ataattattt ttatttactt tagatgtatt tgtattcttt ttcttgttac
    16621 ctttcttttt atcatccttt tttctatttt tagggtaagg aatgactttg tgtgtattat
    16681 caagtttgat atttttattt ttttgaatat tgttttccag gctagctttt ttttctccaa
    16741 attgaatagt tttattggta ttgttgttat ctttcaatgt taatctccct cccttaaact
    16801 ttccctttag ataatattac tactaaccta tggaatatac aataaataat atataagtaa
    16861 taaaatttta taggtataaa tctctttctc cacgctctaa ggcagtcata ttgttagata
    16921 ctatgtttct tatatcttct ctttctatct tacctatttc ttctgcgata agttttttac
    16981 ccataatttt aagttcttca tctccataat cgattaaaaa ttcatttaat gtagtaagag
    17041 catttggtgt acatacatta tggatttgtc gacttttagc taagctcatg aatctatcgc
    17101 cagttctacc tttacgataa caagctgtac aataactagg tatatatcca tcagtaatca
    17161 gttctttaag cacctcaaca gggcttctat ggtctccaac ttcgaattgt tctgtattgt
    17221 caccatcttc atatgcttta tatcctccaa cacctgttag agaacctgca ctaatttgtg
    17281 atacaccata ttttagtaac tcatttctca tttcagcagt ttctctagtg gacattatga
    17341 ttccagtaaa aggaacagct aatctagtta tagctactat ttttttgaac atatcgtcag
    17401 aaactaagtg aggaaaatct tctaagctca taccttcagc ttttttcaat ctagggaaag
    17461 atatagtatg gaaacctaca ccaaattttt cctctaagtg ttcgttgtgc atcataagtc
    17521 ctagaacttc aaatttaggg tcagataaac caaataaaac tcctgcacca acatcatcaa
    17581 caccagcttc catagctcta tcaaaagctg ttaaatggta ataatagtca ttttttatag
    17641 attgaccatg catttttata aatgttggtt tgtgataagt ttcttggaat aatatataag
    17701 ttcctattcc tttttctttt agtaatttat actcatctac acttgtagct gctatgttaa
    17761 cgtttaccct tcttatattt ccgttttcat tatatgttga ataaattgca tctaagcaat
    17821 ctaagatgta atttatatca caatttttag ggtcttcacc agcttctaga gcaagtcttt
    17881 tatgacccat tttttctaat attttaacct cttcccttat ctcttccatg gtaagttttt
    17941 ttcttttaaa tttgttgcat ttactaaaac cgcaataaac acattcattt acacagtagt
    18001 ttgatacata aagaggggca aataaaacca ctctattacc atatatttca ttttttattt
    18061 gcccagctat actaaataat ctatctagtt gttttttatc ttctacttca agaagtgtag
    18121 ctatatcttt ataactaagt ttttcccttc tatcagcctt atcaagtacc ttttcaatat
    18181 catcagaagt tgaatttttt gcatcttcta ataaagaatt aataagttca tggtttatga
    18241 acatttacaa acgcctccat tcaatattat caccgcgaga atagtcaact tcaagtccta
    18301 atgacttgat gttttcttct aaactttggt gatattcaca agcttcttga ccagtaaagg
    18361 ctttgttatc gtaaagagaa tattgttttc taaattcttg aggagaaagg tttggcataa
    18421 ttacattgca accagctaaa agacctgcat ttctaccact agggtttata gatgccaagg
    18481 ctgttgtagc gggtaataaa acttttggaa gtagaagtct tgtaattgaa agcataagta
    18541 atgtcttctc taaatctcca tgtttataat ccttaaaaat tgtatcatgg tgtggaatga
    18601 aaggtcctat tccaaccata tgaggattta actcttttag gtataataaa tcacgaacaa
    18661 ggtcttcgtt gttctgaaat ggtgattcaa ccataaatcc agcacctacc tgataaccaa
    18721 tttctttaag gttttttagg cattcttttc tagttctaag ctcaatgttt ggcggatgta
    18781 attttttata atgactgtct gtagcagttt catgtcttaa aagatatcta tctgcaccac
    18841 ataaaaagta ttttttatac gaatcataac tcttttctcc tagtgataga gttatggcac
    18901 aatcctcata tttatttttt atacttgaaa caatttcaca tattttatca tcagtaaaat
    18961 atgcatcttc accaccttgc aaaacgaatg ttctgtatcc tattttataa cctatatcac
    19021 aacattcaag tatttcatca agtgaaagcc tgtatcttat gatattttta ttagaagatt
    19081 gtattccaca atagtagcaa ttatttttac aatagtttgt aagctctata agtcctctca
    19141 agtagactct atttccataa tgtttatttc tcatgtcgct agcttgttta taaagatagt
    19201 ttcttaaatt ttgttcgaag ttatattcct cattgtcgat attttctatt aaatatagta
    19261 gttcatcaaa acttaatgaa ttaatgctgt aaagtttatc tataaagtat ttaatcttat
    19321 ttttagtcat attcaaatat ataattatta agtatagtta aatttttaat aattgaaaag
    19381 ttcaagtgat ctttccaaaa taccttttac atatgctatt aagataccat agtttacaat
    19441 aggtacattg aaagattttg ccttttctat tctagataac attccagctc tattcatcat
    19501 acaagctcca cagtgaacta caagggcata tttatttata tcttcagtga aggatactcc
    19561 agaactaaat tcaaaagtaa tgtttttgcc agttttcttt ctaatcatat taggaatttt
    19621 tactgttcca atatcatcag tttgtctatg atgggtacat ccttcggcca tcaagatttt
    19681 atcaccatct tttaaatttt ctaaagcata agcgccatta atcaattctt taaggtctcc
    19741 cttttgtcta gcaaacaata tagaaaaaga tgtaagagga atatcttttg gtgtatcttt
    19801 atctacttgt ggaaatactt gagagtcagt aataacaagt tttggttttt tgcctaaatt
    19861 agatagagtt tcttttaaac tatcttcttt tgtaactata gaaattgcac cactatccaa
    19921 aatatctctt ataacttgct gttgaggaag tataagtcta ccttttggag ctgctttatc
    19981 tattggaaca actaaaacaa ttaaatcatt tggttcaatt aaatctgata ccaatttgaa
    20041 ctcagtgcta tccttaggca aaacttttat tatttcattt tttaaatttt caattccaat
    20101 tttatctgtt gatgagacag aaacaactgg acatttaact ttatttttag ttaagtttaa
    20161 aatttctgat tgattcttaa tagtatcaat tttgttgaga attagaatat gagggatatt
    20221 cttatcatta aacttttcaa ttagtgacaa atcttcttgt gatataccaa tctggcaatc
    20281 cactactagt aaagctatgt ctgtcttttc taaaacatct agtgatttac ctattctaag
    20341 ttcaccaagt tcaccaacat catcaagacc agcagtatct ataagaacac aaggtcctat
    20401 aggaaggatt tccattggtc taaatactgg gtctgtagta gttccagcaa tatctgagac
    20461 tattgcagca ctttgatttg ttatagcatt tattatgcta gatttacctg catttctttt
    20521 accaaagaga cctatatgta ctcttaccga ttgtggagta gaatttagac tcatttgtac
    20581 tcaccacctt aaacttcttt ttttgatata ctagttttta cagatacatg aggtatgttg
    20641 cccaatttac cagtcaaact attaatagta tccatttcgc caacaactgc tatacatacg
    20701 attgagacac cttcttcttc gcaaggaatg cccattcttc ctttaataat acctttaaaa
    20761 cctgatacaa cctcattgaa tttaaattga cattcttttg gctcttccaa aatagcactt
    20821 ataactgcaa cctttttcat aaatatcctc ctaactcaga aaatatgtct taaacaaatc
    20881 caaaatcaaa aaacttttct ttgtaaagaa aagtttctac aaaagaaaag ttattaatta
    20941 cacaagtata atgtgctata ttaaactatt tccttacaga ttagataact cttttctggt
    21001 agggttagct tgtaaaaatt taatttaaca acaagtaact gaagaaaatt tttatacaat
    21061 ttatataaat tataataaaa attaactaaa gaaaattgaa gttacttata taatagtagt
    21121 aaattgaaat taagtctata gaaaaaccaa tttttgtata aaaataaaaa ttaaagttaa
    21181 ttatttgtta agaattgact ttggtaaaat atataattaa ttattcttta agcatataat
    21241 ttataagtaa ttttagtgta ttttcaatag cttctatatg tgttctttca taatggtgac
    21301 tattgtttgt tccaggacca acacatgcta tttgaacatc ttctccccaa cgaatagctt
    21361 ctgaagcatc tgaaccatag aaagtaaata catcgacagc gtagtctaaa ttattgtctt
    21421 ctgcaacatc aacaagcttg tttcttaaat taaaatcata taatgataaa ttatcttttg
    21481 caactatagt tacaccatgt tcactagatg tttgacttgg tccagtaggt gctatatcta
    21541 ttgctacaaa ttcttttgtt ttttcaggta aacatttaga aacaccatgt ccaatttctt
    21601 cataattact tatataaaag ttagtagtat gcttaggtgt taaattgttt tctttaaaat
    21661 atctacatat ttcaagaacc attgctacag ctgatttatt atctatatat ctagatttta
    21721 taaaaccaga ttctgtagcc tcaaatctat ggtctaggca tacaaaatcc ccaacactta
    21781 ttcctaaatc taatacatct tgctttgtaa atactttttc atcaagtcta ataatcattg
    21841 tttcttcatt tctttcagtg gcagcttttg tttgaccata tatatgagta gaagcatact
    21901 taaatacaac acttcctctg atttttttac cttttctagt tataacagta cagttttctc
    21961 cctctatagc tgcccaacat ccaccaccaa ctttatggta tttaagagta ccatcagatg
    22021 atatttcttt tactatagct ccaagtgtat ccatatgtgc agatattgtt atttgatttg
    22081 aatcatcttc accaggaaga gtagctataa gtgcaccttt ttttgtaaga atagtgtcaa
    22141 gacctaaatt ttcaaaatct tttttgattt ctaaaactgc attttcagta tatccagctg
    22201 ggcttggaat gtccaaatat tttttcatta aatttatagt attagttaaa tttaaattca
    22261 tattgtttcc tcctaaataa atatatttat acaaattata acataaatac a
//
LOCUS       DAMJWS010000072        22146 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMJWS010000072 DAMJWS010000000
VERSION     DAMJWS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.073"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            444..1514
                     /locus_tag="PC399_002744"
     CDS             444..1514
                     /locus_tag="PC399_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593594.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="PC399_002745"
     CDS             1498..2367
                     /locus_tag="PC399_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2593595.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="PC399_002746"
     CDS             2411..3271
                     /locus_tag="PC399_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2593596.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="PC399_002747"
     CDS             3302..4606
                     /locus_tag="PC399_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2593597.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="PC399_002748"
     CDS             4669..5931
                     /locus_tag="PC399_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF2593598.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="PC399_002749"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="PC399_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF2593599.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="PC399_002750"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="PC399_002750"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2593600.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="PC399_002751"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="PC399_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF2593601.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="PC399_002752"
     CDS             13008..13937
                     /locus_tag="PC399_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF2593602.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14151..15947
                     /locus_tag="PC399_002753"
     CDS             14151..15947
                     /locus_tag="PC399_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF2593603.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16087..17868)
                     /locus_tag="PC399_002754"
     CDS             complement(16087..17868)
                     /locus_tag="PC399_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2593604.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17891..19777)
                     /locus_tag="PC399_002755"
     CDS             complement(17891..19777)
                     /locus_tag="PC399_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF2593605.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19794..20291)
                     /locus_tag="PC399_002756"
     CDS             complement(19794..20291)
                     /locus_tag="PC399_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF2593606.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20609..22090)
                     /gene="cls"
                     /locus_tag="PC399_002757"
     CDS             complement(20609..22090)
                     /gene="cls"
                     /locus_tag="PC399_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2593607.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8524 a         2965 c         3697 g         6960 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaattttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tactttcaaa tcaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa attacattgt gatagcaaac aatgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacctc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt taccatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tggaaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacagaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacaaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaactgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtactca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacaca attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggttag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtaat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag ggccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtttaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggggta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcggg aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga attgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactggg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatatat aatgtttatt
    14041 aataagatat attatataag gataatttaa agtacagtgt tagattataa taatactaga
    14101 ttaattttat atttgagttt cattatataa caagaaagtt ggaatttttt atgggaatag
    14161 aatattttag taaactttta gaaagtagta ctgaaaaaga attgctctta agtaaaaagt
    14221 caaataacat aagtacagca agattaatat cttttttgat agtgatagca gggtttgcta
    14281 ttggatttta caataaaaat atagtaggta tttttgttgg agttttatca ataataatat
    14341 ttatagcact cttagttatt catagtaaag taaaagaaga agaaacatat tttaaaagta
    14401 agagcgaagt attaaataag tatattaaga gatttggaga tggttggaag gaatttaaaa
    14461 tagatggtaa agaatactta aaagatgaaa attcgcaagc aaaagattta gacttatttg
    14521 gaagagcatc attgtatcaa tatatctgtg tagctaatac atcttatggg aaaaattttt
    14581 tagccaaata tttgtggaat gaaaatccag atgagaatgt aatcttagaa agacagagag
    14641 ctataaaaga acttttatca aaagatgatt tttctattca tattcaaaca ctcagtaata
    14701 taattggtaa agagcaaaaa aacaattcag atagtagtat agaatcgttt atagaatatg
    14761 gagaaaataa gaatgtgtat ataccaaaat ggatgcatat ttttacttgg ggtttaccaa
    14821 ctgcaacaat cttatctttt atattttgta tgttaggatt tttacctgtt ttacctgttt
    14881 ttttactttt tataatccag ttagggttta gtggttttgg atatccaaga cttatgcaaa
    14941 ctcttgctcc attattttca tttagtagaa gtatacaagt atatgaaaaa atgtttgagg
    15001 ttttagaaaa agagacgttt gagagtgcat atttgaaaga attacaagat aaactttcta
    15061 agggaagtgg agtttcaaga ggtgttaaac aattaaattc aataggaaat gctgtaaacc
    15121 taaggtataa tcagatacta tatataattg cctgtggtgt tttgatgtgg aactatcatt
    15181 gtgcagaagc tttggagcga tggaaaggta tatatggaaa tcaaattaga gattggtttg
    15241 aatctattgg agaatttgaa gctctaataa gtttaacagt tgtttctcat gtaaaggaaa
    15301 atacatgttt tccagtaata aaatatgaag atactccaag attgaaagtt gaagaggtat
    15361 accacccatt aatagcagaa aaaagtgtca tagcaaattc gatacaatta aattctcaga
    15421 cttgtattat cacaggttca aatatgtctg gaaagacaac atttttaaga agtatcggtg
    15481 taaacttagt gctggcatat gcaggtgctc cagtatgcgc taagagtttt gatgcgacct
    15541 gtatggctat atttacttct atgagaatac aagatgatgt aagtcaagga atatcaactt
    15601 tctatgctga gattcttaga ataaaatcaa tgattcaata tagtttaaaa gaactgccaa
    15661 tgttggttct agtagatgaa atttttaagg gaactaactc tgctgataga attattggag
    15721 caagtgaggc agtaaagaaa ttatcaaaac cctgggttat aagtatggtg acaacacatg
    15781 actttgagct atgtgacctt tcaggtagtg gagatgttga agttgtaaac tatcatttct
    15841 cagagtacta tgtagacgat aaaattcatt ttgactataa gataaaagat ggaagatgta
    15901 aaactacaaa tgcaaaacaa cttatgagaa tggcaggtat tctttaatta atagataggt
    15961 taattaaaga ataagtctat ttagaaatat aatttaattt gtgaaattaa taaggattgt
    16021 cttaaattac gtaaaagtat tttaagacaa tcctttttta agaataaatt ttatattttg
    16081 ttatgactac ttagcttctt cacaagcaca ttcttgtgga cactctccat agacacaact
    16141 cttatcaaag taatgagtat gaaggtattt atgggctaca tcactatttg gttctccaaa
    16201 gaagtcttta taaatagctt gtaaatcagg attctcatga gatttacgaa tagccttatt
    16261 tctatctatt tcatataacg catttgcacg tctatcaact atttcagtat taccatggtg
    16321 ataaggttga ccagcacctc caacacatcc accaggacat gccataactt ctatgatatg
    16381 gtatttacat tctccacttc tgaccttatc cataatcttt ctagcgtttt taagtgtatt
    16441 tacaatacac acattaactg tagttccatc aacatctata cttgcttccc taattccttt
    16501 aagacctcta acattagtga agtttacatt atctaaagtc ttattagtta tcttctcata
    16561 tgaagtacgt agggcagctt ctaaaactcc accacttgct ccaaatatag atgcagcacc
    16621 tgtagatttt cctagaggat tatcaaaatc ttggtcttga agattaggta aatcgattgc
    16681 agcttctttt atcatcttag ctaactctct agtagttata gaaaggtcaa catctagtat
    16741 tccagattca cttaattctt ctcttgaagc ttcatatttt ttagctacac aaggcattac
    16801 agatacaaca taaagttctt caggattgat tcctaatatt ttaggtgcat agtagttttt
    16861 agctatagaa ccaaacatac cttgaggaga cttacagcta gatgctaagt ttaacttgtc
    16921 tggataattg tgctctaaga agtttaccca tgcaggacaa caacttgtta atataggaag
    16981 attttcacct ttttggattc tttctataaa ttcagtagct tcttccatta tagtaaaatc
    17041 tgcaccaaag tttgtatcaa atacatgttc aaatccaagt gcttttaaag cagctaccat
    17101 tttccctgta gagattgacc ctggttctaa tccaaactct tcaccaatag caactcttac
    17161 agcaggagca acttgcacta ctatagtctt ttctttttta tttaaaacat cccaaagttt
    17221 aggtacatta tctacttctg ttaatgctcc agttggacag acagatatac attgaccaca
    17281 gaatgtacac tctgtttcaa ccatatcaga gttaaagaat gtactaacta aagtattaaa
    17341 tccacgatta actccagata atgctcctac tgtttgtatg tcattacata cagtctcaca
    17401 tcttctacat aatatacact tagaatggtc tttaactaaa gatttagtag aagtatcttt
    17461 acctgcaaaa gatttagaac cttgatatct tatttttctt actcctaaat cagcagctat
    17521 agtctgtaat tcacaatctc catttttttc acagacaaaa caatcttgtg gatggtctga
    17581 taacaataat tcaacaatag ttctacgagc atttaatgct ttagcactat tagtctgaac
    17641 tttcatacct tcctttataa ctgttccaca agctggaact aatcctctct ctgtctcaac
    17701 catacaaaca cgacaagaag cacaagtgtc aagcttatct atttcattca tatgtaaatg
    17761 acaaaggttg tgtattttta tatttattaa tttcgcagca tctaagatag aagttccact
    17821 aggtgctgaa acgtgtttgc cattaatagt taaattaact aaactcattg ttattcctcc
    17881 tccaaataag ttatttcttg attatagcgt taaatgtaca attatctata caagcaccac
    17941 attttagaca cttagaagta tctattgtat gttgttcctt aacagaacct gttatacaac
    18001 cagcaggaca cactctagaa cattttgtac aacccttaca gtcatctgtt ataaagtaag
    18061 aaagtaagtt ctgacattta ccagcaggac atttcttatc tacaacatga gctatatact
    18121 catctttgaa atattgaaga gtacttaaaa cagggtttgg agcagcttta ccaagaccac
    18181 ataatgaagc agtttgtata gtttcagcaa ggttttctag gtcatctaaa tcttgtagag
    18241 taccattacc ttctgtaatt ttagttaata tttctaataa tcttgtagta cctatacgac
    18301 aaggtgtaca tttaccacaa gattcttcaa cagtaaactc aaggaagaac ttggctatat
    18361 ctaccataca gtcagattca tcaagaacaa gcataccacc agagcccatc attgaaccga
    18421 tagaacttaa tgaaccaaag tctataggag tgtctaaatg ttcagttggt atacatccgc
    18481 cagatggtcc acctgtttga acagccttaa atttcttacc ttcagggatt cccccaccaa
    18541 tttcaaatac aatatctctt aaagtagtac ccattggaac ttcaacaagt cctacatttt
    18601 ctacttttcc accaagagca aagactttag taccactaga atcttcagta cctaagttgg
    18661 caaaccaatc tccaccctta agtataattg caggtatgtt agcaaaagtc tcaacgttat
    18721 ttaagcaagt aggagatttc catagaccac tcttagaaga actataagtt ttcattctag
    18781 gttcaccacg tctaccttca attgaatgca ttagcgctgt accttcacca caaacgaaag
    18841 cacctgcacc atattttaat tctaatttaa agttaaatcc agtgcctaag atattttcgc
    18901 caagtaaacc gtatttttca gcttgagcta tggctacttt taatctttct atagattttg
    18961 gatattctgc tcttatgtat atgtatcctg tatcagaacc tatagcgtat ccacatattg
    19021 ccatagcttc tagtacacta tgagggtcac cttcaagtac agatctatcc ataaatgcac
    19081 ctgggtcacc ttcatcagca ttacatacaa caaacttttt atctgttgga ccagctggat
    19141 tttttgaagc agcttcccat ttagagccag tagggaaacc tgcaccacct ctaccacgaa
    19201 ggccagaagt ttttacctct gcaataactt cttgaggagt aagagaagtt aaacatttgc
    19261 ctagagctaa ataaccatca ttggcaatat attcttctat actatctggg tctataagac
    19321 cacaattttt taaagctata cgtaactgtt ttttgtagaa agacatttct tcttgttctt
    19381 caactttttt acttaaagat tcttcctcat ataataattc ctcgacaact gtatttctaa
    19441 ttaagtggct ttgaactatc ttttcagcat cagatggctc aacttttacg tagaaaacgt
    19501 tatcaggata aacttttaca ataggacctt gagcacagaa accaaaacaa cctgtcaaac
    19561 gaacactaac tttatcttgg attcctgatt tctttatttc attttctaat tcactaacta
    19621 tttctagact gtttgaagat gtacatccag tatcacaaca aacaagtaat tctcttctta
    19681 atacattttc cttagaataa ttttttctta agctaagatt aggtttcagc tcatcagcta
    19741 aaacttttaa ttcatcaaat gaatttactt tacgcattat ttattcaccc ctcttaacaa
    19801 ttcaattcta attctctata agtatctaat atttcgctta cttggtcagg tttcacttta
    19861 ccataaactt taccatttac agtaacaact ggagctaaac cacatgcccc caaacatctt
    19921 aaaccttcta aagaaaattt aaaatcacta gtagtttctc caacttttat gccaagctgt
    19981 ttttcaaaag cacttaagat tttgtcagca ccttttacaa aacatacagt tcctagacag
    20041 acactgattt tatacttacc tacaggttct gtagtaaaat aagaatagaa tgtaacaacc
    20101 ccatatacct ttgcaggagc tactccaagt tttctagcaa catgaagttg aacttctttt
    20161 ggaagatatc caaatatacc ttgggcttca tgtagaactt gtattaacgc accttccttt
    20221 gttgcaagag agtcaataaa tatatctagt tcgtcaaata attgcttgtt gtgtgaaata
    20281 aaatcgcaca taacaaatcc cccttttctt tttaaagtta ttatttttca taaaataatt
    20341 aaccatgagt tgaccccaac attatgtggg tgaacaaaac caaaacaata aagaaagaat
    20401 aatatacaac aatttcttta tttataaaaa caatgcaaaa aagttaccta ttaaaaataa
    20461 aaactatact agtctattat aatataaaat aattcagaat agttaaaaaa aataaaaaaa
    20521 atgagctaaa ttcaataaaa aaaaaataat acatataaaa aactttaaaa aataaaccaa
    20581 aatgtaaaat ttttataaaa taaaagattt ataaaactgc tgataatagt cttgaaattg
    20641 attccttgat ttttatataa gtagacctag aattatatat atctaatgtt atttccttag
    20701 aatttaaaat atcattttca aatataattc tttgttccaa tgcttttttt gaagagtaca
    20761 taaatgcatt aacttcgaaa ttaagctcaa aacttctgat atccatatta gctgttccta
    20821 tagagcatat agagtcatct ataactattg tcttagcatg taaaaatgca ttttgaccat
    20881 aagtatagat tttagctcca aactttaaaa gttctccagc atatgaatat gatgcccagt
    20941 atacaaaggg atggtcaggt ttagaaggaa tcataactct cacatcaaca ccagataaac
    21001 aagctatttt taaagtatct ataaaagttg agtcaagtat taaataagga ctctgtatgt
    21061 atatatactt tctagctttc tgaatcattt taaggtaacc atatttaatc tcatcaagtt
    21121 ctgatatatc tggaccactt gatactatct gaatcccaat attttcagaa ggatgattag
    21181 aatttgtatc ttcttctata aagtactttt ctaaatctaa gtcttcttta gttgtatatc
    21241 tccaatcaag gatgaatctc atatttaggt caattacgca atcaccagta agcttagtat
    21301 gtgtatctct ccatagacca aattttggat ttctacctaa atattcatca ccaacattat
    21361 atccacctac aaatcctacc ttaccatcaa tgacaacaat ttttcggtga tttctatagt
    21421 ttaagttaaa gtttacaatt ttcaagaatg atgggaagaa gcttccaact ttaacaccac
    21481 tttctttaag tctggaaagt gtactatttt ttagtgttct accaccaact gcatcaaata
    21541 aaagtcttac ttcaacacct tctttagctt tgtctataag aatgtctatt atttcagttc
    21601 cgattttgtc atctttgaaa atgtaaaatt gtatgtttat atatttttta gcttttttaa
    21661 gttcttcaag aagactgtta aaaaattgac tagattcagc ataaatccac acatcgttat
    21721 tattggtata atgagcatta tttgaattag ccaatgcata aatcatatcc ttatgttgat
    21781 gtatatctga atcaatttca gatgtagatt ggagtttaac ctgagtatct agtatattac
    21841 ttttgattat cttatcatcc ttttctttca atctaaacat attgtttttt gaaatatttc
    21901 tgccaaaagc tatataaagt atgaatccaa gagcaggaat caatactaat ataagaagcc
    21961 atgttaaagt tgtatttatg tttctttttt ctctaaagat aaggtttagt ataaccatga
    22021 aattaattat atatattgta gtaactgcta tttcatatat tgacatttcg aaaaatgata
    22081 tatcaaacat aaaattccct ccttcaaata tttattaaaa taagtaatta tatataataa
    22141 cacaaa
//
LOCUS       DAMJWS010000073        21968 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMJWS010000073 DAMJWS010000000
VERSION     DAMJWS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.074"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            36..371
                     /locus_tag="PC399_002758"
     CDS             36..371
                     /locus_tag="PC399_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2593608.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            508..1785
                     /locus_tag="PC399_002759"
     CDS             508..1785
                     /locus_tag="PC399_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF2593609.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1906..3294
                     /locus_tag="PC399_002760"
     CDS             1906..3294
                     /locus_tag="PC399_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2593610.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3498..4211
                     /locus_tag="PC399_002761"
     CDS             3498..4211
                     /locus_tag="PC399_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF2593611.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4329..5198
                     /locus_tag="PC399_002762"
     CDS             4329..5198
                     /locus_tag="PC399_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2593612.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKELVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5353..6498)
                     /locus_tag="PC399_002763"
     CDS             complement(5353..6498)
                     /locus_tag="PC399_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2593613.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(6831..7607)
                     /locus_tag="PC399_002764"
     CDS             complement(6831..7607)
                     /locus_tag="PC399_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2593614.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8189..9163
                     /locus_tag="PC399_002765"
     CDS             8189..9163
                     /locus_tag="PC399_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2593615.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9261..9566
                     /locus_tag="PC399_002766"
     CDS             9261..9566
                     /locus_tag="PC399_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2593616.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9722..10882
                     /locus_tag="PC399_002767"
     CDS             9722..10882
                     /locus_tag="PC399_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2593617.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            10932..11834
                     /locus_tag="PC399_002768"
     CDS             10932..11834
                     /locus_tag="PC399_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2593618.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            11914..12807
                     /locus_tag="PC399_002769"
     CDS             11914..12807
                     /locus_tag="PC399_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF2593619.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      12860..13041
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13269..14399
                     /locus_tag="PC399_002770"
     CDS             13269..14399
                     /locus_tag="PC399_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2593620.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14471..15496
                     /locus_tag="PC399_002771"
     CDS             14471..15496
                     /locus_tag="PC399_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2593621.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15499..16695
                     /locus_tag="PC399_002772"
     CDS             15499..16695
                     /locus_tag="PC399_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593622.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFNLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(16823..17548)
                     /locus_tag="PC399_002773"
     CDS             complement(16823..17548)
                     /locus_tag="PC399_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2593623.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            18026..20125
                     /gene="nrdE"
                     /locus_tag="PC399_002774"
     CDS             18026..20125
                     /gene="nrdE"
                     /locus_tag="PC399_002774"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF2593624.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            20137..21108
                     /gene="nrdF"
                     /locus_tag="PC399_002775"
     CDS             20137..21108
                     /gene="nrdF"
                     /locus_tag="PC399_002775"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF2593625.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            21184..21810
                     /locus_tag="PC399_002776"
     CDS             21184..21810
                     /locus_tag="PC399_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2593626.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         8500 a         2433 c         3871 g         7164 t
ORIGIN      
        1 tttaataaat taaataaaga aaagaggaga ataaaatgta caacatagat gatatgatat
       61 ataattgtcc agtagaagct ttatcaaata ttttaggtaa aaaatgggtg gctataatta
      121 tatggaaaat acaagaagat aaaaaacgat ttggagagct acaaagagct ataccagact
      181 gtagtaagaa aatgttagtt caacaactag attttttaat tgaacagggg attatagtca
      241 accaaaaaaa gttggttaat aatgttgtag agtcaacata ttttttaagt gaatcaggat
      301 tacgattgct tcctgtcatg gaaaaaatga ttatgtggag caataaaaat ttaaattgtg
      361 ataaatacta aaatttactc tgtaagtaat gaaaatttaa ttaagctatg agtagaaaaa
      421 tgtattaaaa ttacataatt ttttagttag attttaattt agaaatggga ttgtaatatt
      481 ggaaataaaa taaggaggaa ttttaatttg caaaatgtaa atttattttt agtatttata
      541 gaaggtatag tatcattctt ttcaccatgt atactgccaa ttttgcctat ttatttgagt
      601 atattgtcaa atagtagtgt agaaaattta aaagaaggaa agacgagttt tataggaagt
      661 tctttattta aaaatactat attttttgct cttggaatct caacaacatt ctttatattg
      721 ggttcatcag taaaagtatt gagtatgttt tttaatgaaa ataaagattt aataatgttt
      781 attggtggga ttataattat tatcatggga ttattttaca tgggaataat taaatcttca
      841 atattgaata gagaaaaaag atttaatgtt aagtttaaag aaatgaaagc aataacagct
      901 tttatattag gttttacgtt tagttttgga tggacacctt gtattggtcc aatattggca
      961 tctgtattag tcatggtttc aagttcaagt aatcatctta gtgccaatct tttaatagca
     1021 gtatatacta ttggttttat tttgccattc ataattacag ctatgtttta cagtaaacta
     1081 tttaaaacta tagataagat aaaatctaac atggaaatta ttaaaaaaat aggtgggatt
     1141 atacttatag tatcaggaat attaatgatg gtaaatggat ttggaagtat aagcaaacac
     1201 tttaatacgt cacaaaatag taaaattgaa agcaagcaag aagaaaataa gcgagaaaat
     1261 tctactgata aagaagaaaa ttctgatggt aatgatagtc aaaaagactc taataatgac
     1321 aataatgata aagggtctaa tgatgaagat agaataaagt ctattgattt tactttaaca
     1381 gaccagtatg gtaaaacaca taaattaagt gattatgaag gcaaagttgt attcttaaat
     1441 ttctgggcta catggtgtcc tccatgtaaa gaagaaatgc cttatataga acaactgtat
     1501 aaagactata ataaaaataa tgatgatgta gtaattttag gagtagcttc acctaattta
     1561 ggtagagaag gttcaaggga acatgtagtt aacttcttaa aagaacaagg ttatacattc
     1621 cctgttgtac ttgatgaaga tggagcatta gcatatcaat atggaattaa tgcttttcca
     1681 actacattta taattgacaa ggaaggatat gtaactcagt atattccagg agctatggat
     1741 aaggcaacta tggcatcatt tattgaaaat cagagaaata aataaaagta tttaagtata
     1801 agtttataaa tcaatttaag gttacaaaaa agtgcctaat tgtaccacct aaataatctt
     1861 gttatagtaa aagtataaac aatacaagaa agggttgaat tttatatgaa aaaaacattt
     1921 gatgctataa taataggatt tggaaaaggt ggaaaaacat tagcagggga tttggcaaac
     1981 aggggattaa aagtagcttt aatagaaaag tcaaacaaga tgtatggtgg aacatgtgta
     2041 aatgtggcat gtataccaac aaaaagctta gaaaattcag caaacagcgt aaaaacaaag
     2101 aatataaatt cttgggatga agtacaagct gaatatgaga aagctataga taaaaaagaa
     2161 actttaatta caaaactcag agaagctaac tataataaat taaattcaaa tgagaatgta
     2221 actatattca caggaatggg aactttcatt gatgaaaaaa cagttcaagt caaaactgaa
     2281 aatgaaattt atgagttagt agcagataat atatttatta atactggttc aagaccattt
     2341 atacctaata ttaagggaat tgaaaataag aatatagtat atgatagtga gtctcttatg
     2401 aatctaagag ttttacctaa aaaaatgaca attataggag ctggatttat aggtttagaa
     2461 tttgctggta tttatagttc atttggagca gaagttacaa tattaaactc taataatggc
     2521 attttaccaa atgaagatgt tgaggattca gaagaaataa taaaattact tgcaaagaga
     2581 aatgtaaaaa ttgttaataa tgcaaatata aaagagatta aagaagtttc tgaattagca
     2641 atagtagaat atgaagtaga tggtaaatct aaagaattaa caagtaatat gatacttgta
     2701 gctacaggaa gaaaagcaaa cactgaaggg ctagggctag aaaatgcagg tatagaatta
     2761 aatgaaagag gatttattaa agtctcagaa actcttaaga ctaataaaga gcatatatgg
     2821 gctataggag atataaatgg aggtccacaa tttacttata tttcattgga tgattatcgc
     2881 atagttatta atcagttatt tggagataag actagaacaa ctaatgatag aaagaatatc
     2941 ccaaattcta tatttataag tccagcattt tcaagagttg gtcttaatgt taagcaagct
     3001 aaagaaaagg gatatgaagt attagtggca aagatgccag tagaagcaat tccaagagct
     3061 aaacagattg gcaaggcaga tgggtttata aaaatagtga tagataaaaa atccaataag
     3121 atacttggag ctagtatgat atgtgaaaat tcaagtgaga ttatccatct tattcaatta
     3181 gctgtagatt tagaagtaga gtatacatat ttaagagacc gtgtttatgc acatccaact
     3241 atgacagaag ctcttaatga tattctatct cctaatatga taaaagaagt ataacataag
     3301 tttaatatat aaatctaaaa ttatcattat aagtagattt tagaaagagg ctatctcaaa
     3361 aaatagagat aagtctcttt tttactgttt aaataataaa aatatatttt aatggtatta
     3421 aaaattatct taaaaggtga aataatctgt atttaatgta gtacagttat atctttaaag
     3481 caaggtggtg aaatgatata aataagatat ctaaaagagg gtttgatgat agtgataaaa
     3541 gatggttttc aagtaaagaa ttaacaagac ttataaaggc taaagaagaa atagaatggt
     3601 tattaaatag ggattacaaa atagacccag tcgttacttt tgttggtgat agatatcagt
     3661 tttctataag acaaagagat gcattaaaaa gggcagtctg tactgatgaa aaaaatatta
     3721 taagacaatc aaagaggctt tctctagata aaatcaaaga agataccgta aatatagatg
     3781 gctttaattt gataatatcc atagaggtag gattatctgg tggtactcta gtaataggaa
     3841 gtgatggcaa tataagagat ttagcaggtc ttaggggaac ctataagatt atagataaaa
     3901 cagaagaagc tttgaatctg ataggtaaat tttttaataa atacaatgca cagaatctta
     3961 aattttattt agatgctcca gtttctaatt caggaaattt aaagtatagg atattagagc
     4021 atgccaaaac ttggggaatt gaaactgaag tagaattagt aaaaaatgca gatgttgtat
     4081 tggaaaaatt agatagagta gttagtagtg atgcagtcat agttgacaag tgtataagtt
     4141 attttaatgt ggctagaggt ataattgaag agtatattaa agaatgtaat atcataaatt
     4201 taaataaata gataaatata ggtgagaata tataagtata tgtagcaata ttcttagtaa
     4261 tacaagtaaa gtttgaaaat aggagaacga atacatatcg aatttcataa aatgttgtaa
     4321 aaagacatat gagaggatgg atatgtgtga aattaactaa actaaatatt aaaaaatata
     4381 gagcacctat atataaatat gctttagcaa atataaattt gaggtctgct aaatctacca
     4441 attcaagtat cataacagta atacctcagg gagccaaaat ggaagtattg gatgaggaag
     4501 atgactggat taaggtgatg tacaattctc aagaaggata tgtgtataag gagttggtat
     4561 cagtaagcga atatgcttgg agtaacttaa acttaagaga agataaatct acaacttcca
     4621 atataattac agtaattcct gaaaaatcta gggttgaagt ccttcaagta gatggagatt
     4681 ggagtaaggt tgtatatgat gataaaatag gatatgtttt taattatttt ttatcaattg
     4741 atgggaataa accaaatgaa ttagattata agtattttta tactgacatg attaaatttg
     4801 ttaacgaaaa taatattaaa agtactagcg attatctaat tgtaacagac cttagaaata
     4861 aatatactta tatttttaag aaagataatg gaggatgggg acaactttac aagtggcaat
     4921 gtactatagg caagcctgag actccaacta taacaggtat attttatata agtggaagaa
     4981 aaccttcttt tggaacggat gagtattcag ttaagtatgc tactagaatt aaaggtggat
     5041 attactatca ttctgtacta tatgattcaa caggaagtta tataatagat ggcagacttg
     5101 gtgaagctct tagtcatgga tgtataagat taagtacgga aaatgcaaag tggatatatg
     5161 ataatatacc agatacaaca acagttataa ttcattaaga aaaattttga gttttttatt
     5221 gggataatga ttcttaaatt aataggatta tatattaact tctcataaag agtactattt
     5281 aaaaagtgta ataagagagt gctattaaca aaacaaagat attttgaggc actctcttat
     5341 aaatatacat aattaagata ttttcataaa caattctata atcttatctt tatcaaagaa
     5401 aacaggatta gcaccagcac aagcatcttt catagagttt tctgcaagca attcaaaatt
     5461 aggattatca acacctacat cttttaaaga ttttgggatt ccaacctctt cagaaagagc
     5521 ttttatctta tctattacaa agtcaacaca ctctttatct gatttattat ttacatccat
     5581 tccaataact tcagctattg gtcggaattt ggcaggggct gatttagcat tttcctcctc
     5641 aactattgga agtagcatgg cattacatac tccatgaggt aaatcatata aacctcctaa
     5701 ttgatgagcc atagcatgta catttccaag acctgcatta ctaaaggcaa ttccatttaa
     5761 aaaacaagca taacacattt gttctctagc ttctatgtca ttaccatttt taacagctct
     5821 tggaaggtat ttaaatatct gctttatagc atataaggca gttgaatctg taacatcata
     5881 agcaccatta gctactacag cttctatagc atgagttagt gcatccatac cagttgctgc
     5941 tgttaaatta gatggtttgc taatcattaa ttctgggtca tttactgtca ttgtggctag
     6001 tgaattggta tcaaccatta tcatctttac atgtctgtct tcatcagtta taacatagtt
     6061 tattgtaact tcagcagatg taccagcagt agtagttata gctatgatag gaagacattt
     6121 tttagaagtt ttgttgatac cttcatagtc tctaatatct cctccattag tggctaatat
     6181 agagatagct ttaccacagt cttgtggaga accacctcct atagttataa cgaagtcgca
     6241 attttctgat tttaatattt taagtccttt attcacatta gttacagttg gattttgttt
     6301 aacttcatca taaacaacaa agtctatatc tatctgagat aaaatatctg ttactttttt
     6361 tacagtacca tttgaaacta gaaatttatc actaacaata aatgcttttt tagttcctaa
     6421 tgatttaatt gaatctttag cttcattaag acatcctttc ccaagtaaat ttattggtgg
     6481 tacataataa atgctcataa ctaaaatctc cttttatttt cattgatttt tatgtaaaaa
     6541 tatatatcta acaattttca ctaatttact cataatatat cgtatcttag attgagatag
     6601 ggttgctggt tcttatataa gaacttaatt cgtaattacg attatgatta gaatttaaca
     6661 tagtattttt atcatgtcaa taaaaatata aaaaatatta taaaaaaaac aaagttactg
     6721 tagttaaatc agcaactttg tttttatagt ataaatcaaa taaatacatt agtttaaaac
     6781 aaaattttta attattatgt tttgatatct tccatataca gaatattaat ctaatttatt
     6841 acaatgtctt cattacgttc ttccatcatt gattgaatat ttttctgagc atttaagagt
     6901 aattttttta tcagttctat tttttcgtca ttagctctaa ataaaggcag tgttacacta
     6961 acagcagctc ttatacatcc attttttctt attggtacgg caagacattt taggtgttcc
     7021 attgattcct cacactctga agctatttgt gttctttgaa cttcaagaag ctggtcgtat
     7081 aaaacatcaa aatctgtaat tgatttttct gtaaaacctt ttagaccatc tggatataag
     7141 cttattagtt tttccttgtc aaaatcacat aaaagagctt ttccaagacc tgtacaatta
     7201 gcaggtattt tttttccaac ataagaaata agtctaatcg gttctggaga atctactttt
     7261 ccaatataaa gaacttcatt attgtcaagt ataccaagct gacaagtttc agaacaggaa
     7321 ttaactatat tagtcatttc gtcaattata tagttaaaaa tagttctttt ttcaaaaaat
     7381 gaagacccta atgtgtaaga acgtatccct atagaatatt tggatgtgtt tttatcaata
     7441 gtaatgtatt ttctagcatt aagtgtatga actataggaa agatgctact ttttggagca
     7501 ttgataacat ctgctatttc tgtaagagta tatccatttt cggattctgc taatgcttct
     7561 aaaatatcta atatcctagc tgttgggcga tgattatctg aagtcataaa tttccccctc
     7621 tttttattaa tttaaagtat tagaatatgt tgattttagc acactatacg atattttaca
     7681 atgtagaaaa taaatatgta aatttatata taaataagct cttgatttag atacagatag
     7741 atattttttt agactgtaca ttaaggaata actaatttcc aaaatatacc taaagaaagt
     7801 aagtgatatt tagttatata atttaagtaa atatatgcaa tagttatttt tatatgtaaa
     7861 tatcaaaata ttatgaactt atattttatc tattttttac actgtataaa aactttatag
     7921 ttttgaatac tatataattt taaatacgct gtatttttgc atataaggaa gtcatttgat
     7981 aatatacaag gtattatcag aaagtttgaa aaaattttga caaaaattta ttttttttat
     8041 tgactcataa ataggaaaat gctatcatta attcaaagat tcgtaattaa aaacttagtt
     8101 cttaaatacg aaccgaaacg gccgttagaa ttaaagctaa aaacttaata agagtaatta
     8161 aatatcatgt tttaggagga ttaactttat gataatggat tttattaaaa aaattcctgc
     8221 aggtatgatg atagtgccaa tgtttatagc agctttcatt aatacctttt gcccagatat
     8281 tgtacaaata ggttcgttta cgacagcagt attttcaagt gttgggtctg caactataat
     8341 tggggttcaa ctggtatgct tgggaactca gcttcaattt agagaaatgc caaaagtttt
     8401 aaagagaggt ggtatacttc ttggttctaa atttgctata ggtgctttga taggtatact
     8461 tataggaaag ctatttggaa tggatggtgt ccttggtctg acaacattgg ctgttataag
     8521 tgcagttaca aatagtaatg gtagtatata tctttcattg atgacttcat atggagatga
     8581 aacagattgt gcaactatga gtttacttac tttaaatgat ggaccatttt ttacattaat
     8641 agctcttggg acttcaggtc ttgcaaatgt accattgttg tcattattgg ctgcaatagt
     8701 gccaatactt gttggtatga taataggaaa ttttgacaaa aaaatgaaag agtttttaga
     8761 gccagggtgt aatttactcg taccttttgt agggtttgca cttggtagta gcataaatct
     8821 tgcaagtata ctaaaaggag gtttatcagg agtattactt ggattaatat ctgtatttgt
     8881 aggaggtata tttatagtat tctgtgataa atttatagga aaacgtccag gatatgcagg
     8941 atgggctgta gctacaactg ctggaaatgc aattgctgta cctgctgctg ttgcattagt
     9001 tgacccatca tggcaaccat atgttgcaac agctacaaca caagtggcag ccgcagtagt
     9061 tgtcactgca atattagtac ctcttataac tagttggtgg gctaagaagt atggatgtcc
     9121 taagttccca aaacaaaatg aggatggtac aataacagct taatttatat tacagtgagt
     9181 tgttggtaaa ataataaatt ctttaattag attttaggat attaataaaa ttatattatt
     9241 tataaaggag agcaagaata atgataaatg cagtgataat tgatgaaaaa gataatgttg
     9301 cagtagctat tgaagcaata gctaaaggta gtgaaattag ttttaaatta aaagacaaaa
     9361 cagtaaaaac gattacagca ctagatgata taactattta tcataaattg gctataaaag
     9421 atatggccaa aggtgataaa gttactaaat atggtgaaca tataggagaa gcaaatcagg
     9481 aaataaaaag tggccaacat gtacatattc ataatgttag aagtgttaga gaggatttgg
     9541 ataaacaagt tatgactaat atataaaata atatttatag agttataaaa gttgtattca
     9601 ttgcttttag tgtcaaatag tttttaaata ggtttttagc tgtttgatta gtgagtgtaa
     9661 taaaaattat aatatataaa aattatagaa agtatataga ctattttagg aggaaatcag
     9721 tatgaaattt tatggatata aaagaccaga tggaagagtt ggtataagaa accacatatt
     9781 aatattgcct acgagtgtgt gtgcatcaga tacaactaga ataatagcat cacaagttca
     9841 aggggcagtt acctttaata atcagaatgg atgttcacaa gtacattcag accaacaatt
     9901 aactatggat gttatggctg gttatgcagc aaatccaaat gtatatggtg taatagttgt
     9961 atctttggga tgcgaaaatt gccaaaatga tttggtagtt gatgcaataa aagagagaac
    10021 aaataagcca ataaaaactc ttgtaataca agaagaacat ggaactttaa aaactataga
    10081 aaaagctgtt cgttatgcta gagaaatggc acaagaagct tctttgctta gaaaagaaga
    10141 atttccaata tctgaactta tactaggaac tgaatgtggt gggtctgacc caacaagtgg
    10201 attagcagca aatccattaa taggtgaatt aagtgataaa cttgtcgatt taggagcaac
    10261 atcaatttta agtgaaacta ctgaatttat aggtgcagag catatactag ctaggagagc
    10321 agtaaatgaa gaagtaaaag aaaaaatatt acatatagta catagatatg aaaattcatt
    10381 aaaattggtt ggagaagaag ttagagaagg taatccatct cctggaaata tagctggtgg
    10441 tttaacaagt ttagaagaaa aatcacttgg ttgtatacat aaaggtggtc atagacaaat
    10501 aagtgaagta tatgattatg ccaaacaaat tgataagaaa ggtcttgtta taatggatac
    10561 ccctggaaat gatgcttcat ctgttgctgg tatggtagca ggaggagcac aaatagtggt
    10621 atttagtaca ggaagaggaa caccatcagg aaatccaata gcaccagtaa taaaaataac
    10681 tggaaataaa ataacttttg caaatatgga agataatatg gattttgatg ctagtccagt
    10741 tatctatggt ccacaaacaa tggaagaatt aacagatgat ttgttaaata tggttgtaga
    10801 tgtagcaaat ggaaaacaat caaaagcaga gtcccttgga tatactgaaa tggcaatagc
    10861 tagagtatgc aactatgtat aatctttaag taagaaaata aatacataga attttaataa
    10921 agggtgagat tatgaagttt gtaagtttta ctgaaggaga aaatgaatgt aaaagaactg
    10981 gagttttttc taaagatgaa ttatttatta tagatgttaa ctctttaaat ctcagtagaa
    11041 attttaagga tttaaatgaa ttaattcaaa atgtatctag tgaagatatt attaagttga
    11101 aaactattat aaatgaaagt acattctata attatgatgt ttttaaaaga gagagatgta
    11161 cggtacatgt tcctatagaa aaaccaatac atgatatctt atgtgtggga gtgaactata
    11221 aagaccatct tgaagaaact caaactcact ttgatgatag ttttgaagaa ccacaaaaaa
    11281 cggtttattt tacaaaacgt gtgacaaagg caataggtcc agaagatgag atagatggtc
    11341 attttgagat aaataatcaa ctagactatg aagttgaact tggagtagta ataggtaaaa
    11401 caggtgttaa tattagaaaa gaagaggtag aagattatat ctttggttac actgttataa
    11461 atgatatttc tgctagaaag ttgcaaaaag aacatgttca atggtttaga ggaaaaggat
    11521 tagatacatt tacctcttta ggtccatgta ttttacataa aagtgcagtt ccttttccta
    11581 taaaacttaa agtatgtagt agggtaaatg gtgaagaaag acaaagttct aatacaggat
    11641 tattcatatc agacgtttca gacatagttt cagaactttc aaaaggtatg accttagagg
    11701 ctggagacat tatagctact ggaacacctt ctggagtagg gatgggattt tcacctcctc
    11761 gatatatgag tagtggagat gtagttgaat gtgagattga gagtattgga attttaaaaa
    11821 attacattaa gtaactgttt taatataatt aatattaaag ctaaatctga tgtattaatg
    11881 aacatattaa aataaatttt aggagtttaa attatgaaag cagaattttt gacaccagtt
    11941 gttacaatat ttgatgaaga aggaaattta gataaagaag gtaataaaaa agtgtatgac
    12001 catcttatag atggaggtgt agatggacta gtcataatgg gaagtactgg agagttcttt
    12061 aatatgtcta tggatatgca aaaagaactt atagatttag taactagtca tgtaaataag
    12121 agagtaaaag tgtttatagg tacaagtcgt atgtgtataa atgaatctat agaactttca
    12181 aactatgcac ataatagtgg agcagatggt gttatgataa taagccctta ttatttctct
    12241 cttgatgatg atagtattga gttattttat agtgaaatag ctaagaatac agaggctgaa
    12301 atatatttgt acaattatcc agatagaact ggttatgatt taagtccaga acttacatta
    12361 agacttgcaa gaaaacataa aaacatagtt ggatataagg atacagtaac tttgatggga
    12421 cataccagag atttaataaa tactatgaaa atagagttcc cagaatttaa ggtttatagt
    12481 ggttatgatg aaaactttat acataatcta atgtctgggg gagcaggttg tatagggggg
    12541 ttatcaaatt taattccaga agtttgctcc aaatgggctg aagcttacaa taatagagat
    12601 atggagaagg ttatagaaat tcaaaaatat attaataaag caatggattt ttacttaata
    12661 accaatccat ttataccagc aatgaaaaaa gctatgaata tcagagggtt acaaataagt
    12721 gattatatga ctgctccatt tgtaaaacca aatgaaaaac aagtatgtga aattaaaaaa
    12781 ttgatgaatg aattaaatat atgttaaatc aaccttgcaa agaggtcaac aataaattac
    12841 aattatctta ataaaaatta aattaaaata atactagggt actatattag tttaataggg
    12901 aaagtgatga aaattcacta cagcccccgc tactgtatac ggatacgaaa tcaaaatcca
    12961 ctgaaattta taaaaatttt gggaagggtg atggaggaaa agccgtgagt caggagacct
    13021 gcccagtatt atataagttc aatttagggt ggagattgta atttataata aaatgaagta
    13081 tcaattcgtt tttgtaaagt tgatatatga acttctcctt attaaattag gagaagtttt
    13141 ttattttatt aaaaggttat acttattaaa gatgacttta ggataacttt aataagtata
    13201 ctttaaaaag catatattat taaagttttt tagccactca taaaaaataa gatattggag
    13261 tgaaagagat gaaatttaaa tcaaatgttt taaagcagtt ttttttaatt gctatggtaa
    13321 tttttatggt aggaggtctt atagggtgta gtaaaagtga caaagagaat aaacaagcta
    13381 gtactggtaa gaaattagaa ttaaaatatg ctagtggatt tagtgttgaa gatttagaag
    13441 atggaattaa aaaagtaact gatggagata atcgagaact gattttaatc ccaaagaaga
    13501 ttaaaattcc tgaaaaatat aaaaatgcta atatagttag aacgcctgtg gacaatgtat
    13561 taatagcatc tacaacacag gcttgttctc tgagaacaat agatgaacta gatagtataa
    13621 aagccgtaac aacagaagag cagtactgga ctattaaaga aattaaagat ttaatgaaag
    13681 atggaaaaat acattacgtt ggttcaaatt tagcaccaga ttatgaaaaa atagtagact
    13741 taaatcctga cttaactata gtgagtagtg gtttatcaga ggctgataca aaatttattg
    13801 acaaattaaa agaattaggg cttaattatg ttgtagacaa tgagtataaa gagcaaaatc
    13861 catttggaag aatggagtgg acaagtttaa tagctgcatt ttatgataaa gaagatatgg
    13921 ctactagtga actaaataaa actgtacaaa aggtagaaga agtaagtaaa aaaataaaaa
    13981 acaaagctaa acctaaagtt gtatgggcat ctgtatatga aggaagtgct tatatagctc
    14041 ctaaagattc atatgtggat aatatgataa aaatggctgg tggaataaat gcatgtgctt
    14101 caatagattc aaatgaaggg cgagtaagta ttgaacaatt gcatgaagtt gcaaaagatg
    14161 cagatatatt tgtatactca tcaagttcag attatgcacc aaatttagat tctataaaat
    14221 ctagtgcacc tgtattggct gatttggatg ttgtaaaaaa cgggaaccta tgggtgtttg
    14281 ctccagatta ttatcaatca gtagataaga cggacgaatt aatagtagat ttagttgaga
    14341 tgttccaccc aggtactaca ggtgaaaaaa tcaaacatta tataaattat gataaatagt
    14401 acagagagga cacaaaaaga taggttttat agattttagt taatatagct ttgctaagag
    14461 gagtatacaa atggatttta tcaataaaat gagaataagg aatacaacaa tatttatagt
    14521 attaattata agtatgatac ttactttttt agtgtgtgta gcatttggct ctgtaaaaat
    14581 tgaatttaaa gaagtaatag atgttgtatt aagaggtatg gataatacag aacattataa
    14641 aataatatac aatataagaa tccctagagt acttgcaacc atggtaggtg gagcatgcct
    14701 agcagtatca ggtattttat tacagatatt ttttaaaaac ccaatagttg aaccgtatat
    14761 attaggagta tcttcaggtt ctactttatc agtagctctc atgattttgg gaggatacac
    14821 attgggattt gagagtgtaa attcaatgag tatatttgta gcagctttta taggagcttt
    14881 aattgtaatg tctatagtca ttttatttgc aaataaggta aagaatataa caactctttt
    14941 aataatagga attatgaccg gatatgtatg tagtggtata acaaatgtgt tacaatcatt
    15001 tgcaaatagt gataaattaa aagaatttac tatgtggtct atgggaagtt tttctggatt
    15061 tacatgggaa aaggtagaaa tacttataat agttggtata ttaggtcttt tgatgactgt
    15121 attcataata aaacctttaa atgctttttt actaggagaa gagtatgcaa aaagtattgg
    15181 cataaatata agatttttta gagttttgat agtatttata tctagtatat tagcagcaat
    15241 agtatcagca tttgcaggac ctatagggtt tattggactt gcagtgccac aaatttcaaa
    15301 aatgatattt aaaacatcag ataatagagt tttgatacca gtagtaatat ttttaggcag
    15361 tatagttaca gctctttgtg atttaatatc aagagtaatt ttatcaccag tagaattacc
    15421 aataagtacg ataacatctt tattaggagc tccaattgta gtaatgttat tggtgaaaag
    15481 gaaggatagg gcttagacat gttgaaaact aataatttaa gtgtaggata caataacaaa
    15541 gttgttataa gtaatataaa tgtagaagta aaaaatggag aaatactatg tcttttggga
    15601 agtaatggag ctggaaaaac taccctatta aggtccttat ctaaacttat atcaccaata
    15661 aaaggtgaaa tatatttgaa tggtgtaaac ataaactgta tatcaagaaa agcattatca
    15721 aaaaaaatgg ctttagtact tacaaataga ttattaggtg atttaatgac tgtacaagat
    15781 atagtcaata taggaaggta tccatataca ggattttttg ggagtctatc taagaaagat
    15841 ttaataatgg tagatgaagc attggagtct gtagatgcct tacatttaaa gaaaagatat
    15901 tttgatgaac taagtgatgg agaaaaacaa aaggtgttgg ttgcaagggc tcttgttcaa
    15961 gaaccagaaa taattatatt agatgagcct acaactcact tggatataaa acatagattg
    16021 gaactcataa acatactgaa aaaattaagt aaagaaaagt caatatcagt aatactttca
    16081 ttgcatgaaa tagacatagc attaaaaagt tgtgataaag ttgctcttat aaaaaataat
    16141 aaagtcatag cttatggtca gcctgaagat gtagtagatg aggatattat aaattcgctt
    16201 tatgaattgg atgataaaaa ctttaatagt cttttaggtt cagtagaaat tagtaataaa
    16261 tctaaaaatg aagtttttat aattggtggt gggggtaaag caacaccaat ttatagagct
    16321 tttactaaaa aaggtatagg tctgtattct ggaataatac atgaaaatga tattgattat
    16381 gaaattggta gaactatggg gataaagatg tttacagaaa atccatttga gcctataagt
    16441 gatgagagtt tcaatttggc tattagaaat ttaaatgatt ccaagattat aatagataca
    16501 ggatttagtg taggagagac aaataagagg aatatagata ttataaaaga ggctttaaaa
    16561 ttggataaaa aagtttattc ttttagaaat agagatgaga gtaagaagta ttatgacagt
    16621 ttggatagta agatagaaca tatagataaa gtttctcaaa taatcaatag tgcagatata
    16681 aataatttat tataatttat gaaataaaat gtacttatgt gctatagaaa aaagagtata
    16741 gattataatt tttttgaaat tacaatctat actcttttgt tattttgttt atgtatttat
    16801 atatttataa aactttagca ttttatagat aaccaatagc ttcctctcta gttaaaaaaa
    16861 agtgaatccc tgtagtcgat tctttccatc tattttcatt aaaatcttca gctacaatcg
    16921 tttctcctac tctatatata aaattttcat cagcatatgc acgagcactt ttaaaagatt
    16981 ctttaccatc tatactagta attgacagta cttttgcctt atcacatcta caagcgtttg
    17041 atgtagcaga cactcttttt gcgtcgctgg gtattaataa ttgtaccatt ctaaaattaa
    17101 aacatttttt atagcctata aatgctcctg tttcaggaca atacaattta aaaaaacggg
    17161 tactttcatc tgatattact ccatttaaag tagcatgttc taaatttgaa ttttctaaat
    17221 tagctcctct aatatctgag ttagataagt cacaaaatct aaaacttgca ccacttaagt
    17281 ctgcattttt taatatagaa ttttttaatg aacaatccga aagataagaa ttattaaaaa
    17341 tagagttttc taaatctgct ttttctaaat ttaaattgat aaggtcagac cacgaaaagt
    17401 caatattact taagcagtat cctcttaagt ccatattttc taatcttaat tcttttaaat
    17461 ctgccttttc tccagcatta ttgtttttaa gccataaata atgtttctca agtgtttttt
    17521 ctaattcttg tttagaaata gttttcatta aaatcacctt cataaattta tttttatata
    17581 tgtagtagca aaatagtcat atatttaatt atataacaat aaataaaaaa cttctaaata
    17641 atatataaaa tatcatcttg aaagtacttt tttcaatata tcagaatata tttttaagct
    17701 atttagagat aatttaaatt tttaaacagg acataataag cttgtattgt tttatatttg
    17761 agttgataaa aatttcttta aataaaaata atgtcaatta taaagtaata aaagtttaga
    17821 tataaaaaaa tttgtgtata tactatatgt agtatataat agataaagca ctgactatat
    17881 gtagtgcttt tgtttttgaa aaatacttat tgcatttcta taattataga ttaattttgg
    17941 gatttcaact gataagtacc aaaagaaata ataagcaaat taagtgaaat tatggcacaa
    18001 gattatgtta aaaggacggt aaataatgaa ttggatagag cttaacaatc aagtgataat
    18061 taaaaatgaa caaggcaagt accaattgga aaaggataag gaagccttaa gtagttacat
    18121 agaagaattt atacagccaa agttgagaaa atttaataac ctagaagaaa gattgaagta
    18181 tttaatagat gaaggttatt attcaaaaga agtaataaat cagtatagca tagatgttat
    18241 taaaaatata tatgatatga ttgataaaca taagtttgaa tttcaatcat atatgagtgc
    18301 aaataagttt tatcaaaatt atgcattaaa gagcaatgat ggaaaggaaa tattagaaac
    18361 atataatgat aaagtgttaa ttgtagcttt gacactagga aatggtgata aagatttagc
    18421 tttaaacttg gcagataaaa tagtaaaaca agaatttcaa ccagctacac ctactttttt
    18481 aaatgctgga agaaaaaggg ctggagaaat ggtatcatgt tttttactat ctgtagaaga
    18541 tagtacagaa ggtatatcat atgctatatc ttcatcaaat cacttatcaa agattggtgg
    18601 aggagttgca ctaaatttat ctaaacttag agcatctggc gaatctatca aagatataga
    18661 aggtgcagct ggaggtgttg ttggagtagc taaaatgtta gaacaatcat ttagttactt
    18721 taatcaaatg ggagctagac aaggttctgg tgcggtgtac cttactgtat tccatcctga
    18781 ttttgaattg ttgatggata ctaaaaagat aaatgcagat gaaaaaataa ggcttgctac
    18841 attatcatta ggagctataa taccagataa atttatggag ttggcagaaa aaaatgaagt
    18901 agcatatgca ttttatcctc atactgtgta taagaaatat ggagtttctc ttgatgagat
    18961 agaaatggat gagtggtatg ataaattggt taatgatagt gatataagaa aaaaagaaat
    19021 aaatcctagg cagatgttga caaaaatagc acagatgcaa caagaaagtg gatatccata
    19081 tgtagtgtac atagatacag ctaatagaga acatacatta aaagatgttg gaattattaa
    19141 aatgtctaac ttatgttgtg agatatttca atatcaaaca ccttcagaaa tagaggggta
    19201 tggaggaaaa aatgaatggg gacaagatat aagttgtaac ctaggttctt taaacatagc
    19261 taatgtaatg gataataaaa ctatagaaag tacagttgaa acagcaataa gagcactgtc
    19321 atttgtagca gatagcacag atataaaacc agtgccaacg gtaagcaata gtaacagcaa
    19381 gtctcattct ataggattag gagctatgaa tttacatgga tacttagtaa gagaaaatat
    19441 tttatatact tcagatgatg ctatagattt ttctaatgta ttttttgcaa tggttagata
    19501 ctattcaatt aaagcttcta tgaaaatagc tatagaaaga aatcaaactt ttgaaggatt
    19561 tgataaatca gaatatgtta agggaagaaa tagcaaggtt ttatcaaaat actatgaaca
    19621 atcttatcta cctaagtcag aaaaggtaag agcattattt gaagggattt atataccaac
    19681 aaaagaagac tggacaaaat tattggatga ggtaaaagaa aaagggatat ataatgcata
    19741 tttaatggca gtagcaccaa ctcaaagcat tagttatgtt caaaatgcta catcaagtat
    19801 aatgccaatt actgagccag tagaagtaag aacatatgga gactctacta caatatatcc
    19861 aatgccattt ttaacaaatg ataatatgtt gtattatcaa tcagcataca ggatggatat
    19921 gagaaaggtc atagatttag tggcaacagt tcaaaaccat gttgaccaag gtatatcaac
    19981 aacactgttt gttacagatg aaaagacaac tagagatata gctagacatt acatatatgc
    20041 ctataaaaaa ggtttgaaaa gtttatatta cacaagaaca aagatgacaa gagatactca
    20101 tgagtgtcta gtgtgttcag tatagggaga gaaattatga catttaattt acataaaata
    20161 cataatgcag ttaactggaa tagagaagaa gatggattta cacaagcttt ttgggaacaa
    20221 aatgtaaaac aattttggct tcctgaagaa atatctgttt ctaaagacat aaaggtatgg
    20281 aatgaactaa gtaataaaga aaaggaatta tataaaaaag tattgggtgg tttaacatta
    20341 ctggatacaa aacaggggaa taatggaata ccatctatga tgagtttaac agaaaaccta
    20401 caaagaaaag ctgtcttgtc ttttatgggg actatggaag aaatacatgc aaaaagttat
    20461 tcttctatat ttatgactct actttctaat ttagaaatag atgaattatt tgaatggata
    20521 gaaacagaac caacattaca aagaaaggct gatttagttt tagctcaata cgaaaatact
    20581 acaaatcaag agggattata cctatcaatg gttacaagtg tatttttaga gagtttcttg
    20641 ttttattcag gattctttta tccattgtat ctatctggtc aaggaaagat ggtagctagt
    20701 ggagaaataa tatcgcttat tcttagagat gagtctttac atggtaagta tataggattg
    20761 ctagcacaag aaatatacga ttcatttgat aaaatggata aggagatgtt agaagagaag
    20821 atgtattcta tactttatag ccttatggag aatgagatag agtatacaaa tgttatatac
    20881 agagaaagtg ggcttgaaaa agaggttgtg aactttttaa aatacaatgc aaatagagct
    20941 ttagaaaatc tagggtttga gaagctatat actgtagatg ctataaatcc aattgtatta
    21001 aatggtctta gcactgaaac caaaacacat gatttttttt caactaaggg taatggatac
    21061 caaaaaggag tgtatgagga attagaggat gaagatttta taatataatt tataattatg
    21121 acacactgtt gtcactttta gcgtgataat atcaatgtat aaaatatata tggaggtatt
    21181 tttatgaaat atgaatggag aaaaagagat aaagagttat atttacctaa aaagaatcct
    21241 gtaatagttg aaataccaga acagaaattt atcatgttaa aagggagtgg agacccaaat
    21301 tctaaagaat ttactgaagc aataggagta ctttattctt tggcatatgc aataaaaacc
    21361 atgcctaaaa aaggaattgt accagaaggg tattttgact atacggtttt tcctttagaa
    21421 ggtatatggg ataagggaga aaaaagtaaa ggcttagatg tacttattaa agaagattta
    21481 atttacacta taatgatacg tcagccagat tttgttacac aagacctgtt ggataaagct
    21541 actgatatag taaagaagaa aaaacctcat aatcttcttg aaaaagttag atttgggtct
    21601 attaatgaag ggctatgtgt tcaaatgctt catataggtt catatgataa tgaaccagaa
    21661 agctttgaca ttatgaacga attttgcaaa aaaaataaac ttataagaaa atcttacaca
    21721 catcgtgaaa tttatataag tgatgctaga aaagtatcgc ctgaatcatt gaagacggtt
    21781 ttacgttttg aagtagaaag ggaagtataa atattttgaa taatttataa gagttataaa
    21841 aagtgaagtc tgttgagact tcattttttg atgcaatttt aaaattaaat ttgatgtttc
    21901 ataatatttt aaaaactttc tacttttatt agtgtgaata actgttgcta ttaaataata
    21961 atactaat
//
LOCUS       DAMJWS010000074        21361 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMJWS010000074 DAMJWS010000000
VERSION     DAMJWS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.075"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            666..1697
                     /locus_tag="PC399_002777"
     CDS             666..1697
                     /locus_tag="PC399_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2593627.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1830..3128
                     /locus_tag="PC399_002778"
     CDS             1830..3128
                     /locus_tag="PC399_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF2593628.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3242..3487)
                     /locus_tag="PC399_002779"
     CDS             complement(3242..3487)
                     /locus_tag="PC399_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2593629.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3635..4501
                     /locus_tag="PC399_002780"
     CDS             3635..4501
                     /locus_tag="PC399_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF2593630.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4505..5041)
                     /locus_tag="PC399_002781"
     CDS             complement(4505..5041)
                     /locus_tag="PC399_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF2593631.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5232..5435)
                     /locus_tag="PC399_002782"
     CDS             complement(5232..5435)
                     /locus_tag="PC399_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2593632.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5726..6490
                     /locus_tag="PC399_002783"
     CDS             5726..6490
                     /locus_tag="PC399_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2593633.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6879..8192
                     /gene="prdC"
                     /locus_tag="PC399_002784"
     CDS             6879..8192
                     /gene="prdC"
                     /locus_tag="PC399_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF2593634.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8596..8681
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            9263..11512
                     /locus_tag="PC399_002785"
     CDS             9263..11512
                     /locus_tag="PC399_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDF2593635.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTSDVEKPSDGQVPDGEKPSDSQVPDVEKPSDGQVPDGEKPSDSQVPDGEKPS
                     DGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQ
                     TPDVEKPSDGQMPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINK
                     IVYNFWEDEEDVKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYH
                     DLRFDDITKTLTFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSP
                     YLPAEDFNGQENQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWI
                     ILGQMDEQERLKQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAG
                     REELKPNNTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDG
                     DGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKD
                     HSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPT
                     DDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPP
                     VPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSYVFISVIAICGL
                     AYQCKIKRN"
     gene            11919..13679
                     /gene="prdR"
                     /locus_tag="PC399_002786"
     CDS             11919..13679
                     /gene="prdR"
                     /locus_tag="PC399_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF2593636.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            14108..15988
                     /gene="prdA"
                     /locus_tag="PC399_002787"
     CDS             14108..15988
                     /gene="prdA"
                     /locus_tag="PC399_002787"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF2593637.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            16069..16353
                     /locus_tag="PC399_002788"
     CDS             16069..16353
                     /locus_tag="PC399_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593638.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            16391..17116
                     /gene="prdB"
                     /locus_tag="PC399_002789"
     CDS             16391..17116
                     /gene="prdB"
                     /locus_tag="PC399_002789"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16841..16843,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF2593639.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            17196..17954
                     /gene="prdD"
                     /locus_tag="PC399_002790"
     CDS             17196..17954
                     /gene="prdD"
                     /locus_tag="PC399_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF2593640.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKVRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            17969..18436
                     /locus_tag="PC399_002791"
     CDS             17969..18436
                     /locus_tag="PC399_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2593641.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            18454..18936
                     /locus_tag="PC399_002792"
                     /pseudo
     CDS             18454..18936
                     /locus_tag="PC399_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
     gene            18956..19963
                     /locus_tag="PC399_002793"
     CDS             18956..19963
                     /locus_tag="PC399_002793"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF2593642.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            20083..20964
                     /locus_tag="PC399_002794"
     CDS             20083..20964
                     /locus_tag="PC399_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2593643.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         8523 a         2460 c         3913 g         6465 t
ORIGIN      
        1 atcttatagt ttattgaacc tatagtaatg aattaataaa tagtttaatt ctatgaaaag
       61 atttcctttt atgcaattta tttattgaaa tatgatataa tatataaagg tgtaagtatt
      121 agttgttaaa ataatattta tattagtttt tatgcatact tggtataagt ttttcatata
      181 ttgattaaaa ttgatgttga tttattattt ttagtatttt ttcacctttt gattcatact
      241 ttgtagaatg gtgtattttc taaaactgat tataagacaa gttcattcaa attagcattt
      301 ttatatcata taaataattg ttccccctaa ataagattta gatgtatagt attaaaatca
      361 aaaagtataa aataaaattg atgcttaatc atagataaat attaataaaa tatacaaggg
      421 gattgtctaa taaaaagata atctcctttt ttacaaataa ttctaaattg tgatataaat
      481 ctaaaaattt tatcttaaca tttaaccatt taatgataat aattgatata ataaatataa
      541 attaaatata tggtgtactt attttatgtt tttagcaaga aatatagcat ttaaaagata
      601 aattgtgcaa cttaaatcaa agtttaagat gaactattaa catagatata ttaaaaactt
      661 ttgtaatgga gaatatatgt gatatgaata ataaagttaa ggttgcagta ttagatacag
      721 gaatagacaa agaacatgat tacttaaaag ataatttagt tggaggaatt gcttttgaat
      781 gtatgcatga ttatatcttt atatcagata aatttgatga tgaagatggt catggaactg
      841 cttgtgcatc aataataaag aaagagtatg aagatgtaga gttatttgta ataaaaattt
      901 tgagaaaaga tggtattaca aatataaaag ttcttgaaga agcattaaga taccttttag
      961 acactaacat aagattaatt aatttaagtt tatcagtcat aggagtagaa agtgttaagg
     1021 gcttatttga agtttgttat gaattgttta gaaaaggaaa aataatagta tgttcactcg
     1081 ctaatgattt tgacttaagt tatccagcta tgtttaacaa tgttataggt gtaagaggat
     1141 ttactttaga tggagatgat tcattttggt acaacaaaaa atatgatgtt caatgtgtta
     1201 tggatagcaa ttcatatata agttgtgata taaataattc ctatagatta ccaccaaaat
     1261 gtaatagtta tttatcagca aagcttactg gtaaaatagc taagatatta tcagaaaaac
     1321 caggcataac aatatctgat ttaaatgata agcttgaatc attagcaact agaaatcatt
     1381 gggatagttg tgattttgat gagtgtagta gaattgtaaa ctttaaattg gatttgtatg
     1441 acaaagaaaa tgctcttttt atgaaagttg ctgacgttgt tagggattgc ttagatattg
     1501 aagagaataa tgaaaaactc tttaagggta gcctttttaa taaggagata ggcttggttt
     1561 atgataattg ttttaatctg ttgaaaaagc tagagcatag atttgacata aagtttaatt
     1621 atatggacat ctcaaaatat gacttagttt caatatatac actgactgaa ttagtggaaa
     1681 gatataaaaa cttataaata aagatttgta gctaaattta atgtagaagg tattaaatta
     1741 tatgtattct ttgtcggtag attaaaagta aaaaatatac tataattaga tagcataaga
     1801 gaaaatgcca atacaggggg agttagataa tgaaatatga agaagcatta gagtacatat
     1861 cacaaactaa taaattcggt ataagattag gattagaaaa tatcggaaag ttattagaat
     1921 tattaggaaa tccacaagaa actttgaata taatacatgt agctggaact aatggaaaag
     1981 gttctgtgtg ttcttttgtt tctaatatac taagagagtg tggatataaa gttggtttat
     2041 atacatctcc atatttagaa acttttactg agcgtataag agtaaatgga caaaatatac
     2101 cacaggatga tgttgctaga ataattgagt taataaaaga aaaaatagag attatggtaa
     2161 aagaaggcta tgcatatcca acagaatttg aagtggttac tgcaatggca ttttattact
     2221 attcagaaca aaaggtagac tttgtggcat tagaagttgg tttaggtggc agatatgatg
     2281 ctacaaatat aataacaaaa tcacttgtaa gtgtgatagt atctataagt ttggaccata
     2341 caggtatact tggagataca atagaaaaaa tagcgtacga aaaggctgga ataataaagg
     2401 aaaatggagt agtactagta tatgaccaga ctgatgaagc aaaggatgtt ataaagtcag
     2461 tttgtaaaga gaaaaaagct aaatatatag aagttgattt tgatgatata aacataaaaa
     2521 aatcagatat aaattctcaa atatacgatt gtactgtaat gaaagaaact tatagagacc
     2581 ttgaaataaa attgataggc gaacatcaaa taaacaattc tatcttggca attagtgtaa
     2641 taaaatatct aaaagacata aataaattag caaatataaa tgaagaatct ataagaaaag
     2701 gacttataaa tactaaatgg ccaggaagaa tagaaaagat aaaagaaaat ccaattttta
     2761 taatagatgg tgcacataat gaagatggag caaaatcttt ggcaaaagca cttgataaga
     2821 attttaaagg tagaaaatta accttattaa taggtatgtt agaagataaa gatattgata
     2881 gtgttttaga gatattgttg ccacatttta ataaggtaat aacaacaaca ccaagtaacc
     2941 caagagccat aaattcagat atattaagag aaaaagtatt aaaatatgta gatgatgtaa
     3001 catcaaagca tgagatagag gatgcagtaa attatacatt agaaacatca agtgaggatg
     3061 atattataat aagtgcaggt tcattatata tgattggtac agtgagaact ttagtaaaga
     3121 aattataatt ttataaaata agtatacaaa taaaaatata aaaaagagtt gtctaaaaaa
     3181 tggacaactc tttttatata aaataagttt ttaataattt tactgatgtt tatataagta
     3241 attatataga ttgacgtata gctttagata attggtctgg gcaagaagta ccttttccac
     3301 cacagtcaat accatttaat ttatcagcaa cttcaagagc attttggcca attattaagc
     3361 ttctaagtcc tgataaatta ccattacatc ctcctataaa ttcagcatca acaatcacat
     3421 tattttcatc aacttcaaaa atcatttctc tacaacatac accacttgga ttaaatgtta
     3481 ctttcattaa aaagacctcc attaaatatt atttcttttt ttaacattaa taataatacc
     3541 aaatatatgc gtaataatgc aactataaag cataatacaa gctaatgaaa taatatatta
     3601 ttgtagtata tgtattgtag agaggagggg ttatatgaaa atctatattt ctaactactt
     3661 atcaaagagt attagcatag ttgattattc tacacttgaa ctagaaaaag aaatagtatt
     3721 agaagataat atatatccac accatttttg catagaaaaa gaaaaaaatt taatatacat
     3781 acctagttca agtaatggga tactctatgt cttagattta agtaatgata aaataattga
     3841 tactgtctct ataggaggaa gtcttagtca agttgtactt agcgataatg aattgtttgt
     3901 ggcaaatgaa gattctaata gtatatatgt actagataaa aacacattaa atccaatagg
     3961 aattattgga gttgataata tgccacatgg atttgacttt gatagagaaa gcaaaaagtt
     4021 atatgtaccg tgtataaact cgatagtatg tatagataca attaataaaa atatacaaaa
     4081 gaaaataaat atggacttta gagcttggca tatagaactt gataaacaaa agaaagaaat
     4141 gtatatatca acacttgatg gaaagcttat aataatagat gaagtaagca tggatataaa
     4201 aaaggtatta tatgaatttt tattaccagt agaaataaga tttaattata gtggcaaaaa
     4261 agtttatgtt gctgatttgg gttataataa tgtaagaatt ttagattata caacgagtag
     4321 atacattgga aatattgaaa taaatgggat tccccaagga ttagaaatat ctaaagatga
     4381 aaagttacta tttgtatcag atacacaaga aaattcagta aaagtttatg aaactgcaaa
     4441 taacaaatta ataaaagaaa tcaaggtagg aaaagagcct acaactataa tttgtttgta
     4501 ggctttaatg tatgtctctt ataagtaaat cgataacttc ggcatacata taagatgcat
     4561 tatttttcca gttattgcaa ataagttttg cttgtttctc tgatgaaaca tttagattta
     4621 aatctattaa gttagactga ttttctatga ctctaaggtt gacaataaat tcattatcac
     4681 tttgttttac aaaactagac tttacttgag tatctgctag tagattttct ttgttagaat
     4741 ctatatactt gtctattttt tcagtaacac tagtaggtat tcgattaaag aagtattcta
     4801 gactttctac gcctctttga gtaagagcat aatattctct gtcagagtct ttatatgtag
     4861 tcaaaaactt agattccata agctgagata aaagctgttg taatgaaaaa taattcatca
     4921 tctcagtctc taatactact tgagttattt gagaattagt taaatccatt ttaatttttt
     4981 ctaatatata taaaattaaa agtttgtgat aggctaattc ttcagacgag ttttcaaaca
     5041 taaatatcat cctccgtcaa actatttcta tatataatat attaaactaa tttgtacaaa
     5101 attaaaagaa caatttgtat tgtattatta atctttaata attcatataa aaacaatttt
     5161 ataaataaat atagaaaaac agggctgcta attagcagcc ctgttgatac ccaggtgtca
     5221 aaatttacta tttattttcc agccatttgt ctttcagcca tttcaactaa tttcttagtc
     5281 atatatccac caacatatcc attttctcta gctgttaagt tacctttgtc tatgttttca
     5341 tagttagata aacctatttc gttagctatt tctaatttca tttggtttaa agcagctttt
     5401 gcttctggaa ctactgttct gtttgaattt gacatagtaa atttcctcct caaataatta
     5461 gtttatgatg tctaacaaca cttttattta tgtgatgtta atactaattt ctccaaaata
     5521 aaaataatat atacagaaaa ataaaggtaa aaatgtaaaa aatataaaaa aattaaataa
     5581 atgagtattt tagagagttt aattaggtca atataggttt tattttttta gttattgtct
     5641 tataaattaa atattacagt acttttttga gctgaaaaaa aataaatttt aaatatagaa
     5701 gaaattattt caaaggggga atagtatgaa aagagaagtt aaaaggacta aagctttgat
     5761 atatagtgtt ttattttttt cattattatt agtgttaata tttgtttttg tttatggaat
     5821 aaaatacata tgttctaaat ctgacaattc aaaaatgcct atatataaag ttgatacaaa
     5881 tgagaaaaaa atagctttga gttttgatgt agcatgggga actgataata tggatgatat
     5941 tttaaaaatt ttagataaac ataatgtaaa agcgaccttc tttttagttg gtagttgggt
     6001 ggatgataat gaagaaattg taaaagctat agatgaaaaa ggacatgaaa ttggaaatca
     6061 ttcaaatact catgccaatt taaaggaaat atcaaaagaa gacataacaa aagagataga
     6121 aactacatca gaaaaaatat ataatataac aggaaaaagg actaatttat ttagacctcc
     6181 ttttggggac gtaaacaata aagctatgga catttgtagt gatttaggtt ataaagtaat
     6241 taagtgggat gtagattcta tagactggaa ggagcttgga ccaaatcatg taattgaaaa
     6301 agtaataaaa gaatctcaac ctggttcaat agttttattc catgctaata taaatgatgt
     6361 agataattat ttggatacta taataagtcg actaaaaaaa gatggataca gtctagtcaa
     6421 aatatctgat ttgttgtata aagataatta tgtagttgat tccaatggag tgcaaaaaac
     6481 taagaattaa taagtaataa atgtatattt taagtaaagt tgtgataaat tatatagtat
     6541 atgttagaaa agtgtcgata aaaagacggt gtctattttt tgacactttt tatacatgta
     6601 tgcaatatca ataattatag attaaattga ttaaaaaagt gtctaataat tgactttttt
     6661 ttgatgggtt taaaaattta gaagaattaa tgaatgctgt aagacagtat ttccaaaggg
     6721 tataagtttg tttaaaagtt ggcatagaaa ttgctttgtt aataagcgat atgttctata
     6781 gaaataaaca atatgttaag accttataat agaaaaatag cattaacatt atttgaacag
     6841 cttataaaaa aattaatata cttaaggggg cgtactagtt gaacaaacta tattctattt
     6901 tattaaaaca acacgttggt ggtccagata agccagtagt aagtgttgga gatgtagtaa
     6961 aaaaaggaac tctaatcgca gaaccaactg gattaggggc aaatatttat gccagtgtta
     7021 gtggaaaaat aagtgaaata aatgaccaag cgatagtaat tgaagctgat gaagtacaag
     7081 aagatacttt tgaaccatta aaaggagaag gtatacttga cttaattaaa gaagctggag
     7141 tagtaggaat gggtggagca ggattcccta ctcatgtaaa attaaacata gatttaaatg
     7201 gtggaacaat attagcaaat gcagctgaat gtgaaccttt attagctcat aatataaaag
     7261 aaatagaaga aagaccagaa atagtttatc aaggtataaa atatgctatg gaagttacta
     7321 atgctggaaa aggtatgcta gcaataaaaa gtaagcatcc aaaagcaata gaagctttca
     7381 aaaaagtaat aaagccagga gacaacatag aagttgcaga actagttgat atgtacccaa
     7441 tgggagaaga gagagcaata gtaagagatg ttctaggaaa attacttgag ccaactcaat
     7501 taccttcaga ggctaatgca gtagtaataa acgtagaaac actaactaga atagtagagg
     7561 ctgttgagca aaaaagacca gttatatcta aaaatataac tgtagttggt caattaaaca
     7621 gtggaaaaga atctatagtg tttgaagatg tgccaattgg aacaactgtc ggagaattaa
     7681 ttgaaagagc tggtggaata aaaggtgaat atggtgaaat aatcctagga ggacctttca
     7741 ctggtaaagc tactacttta gatgctccaa taactaaaac tagtggtggt ataatagtta
     7801 ctatgccatt tgttaatgaa aagagaaaaa tgggtctttt agtttgtgca tgtggaccta
     7861 atgaagaaag aatgagagat atagcaacta aaatgggagt tactgatata gtatcagttc
     7921 aaaaatgtaa acaagctcaa gaaattaaag gtgcattaaa atgtgaaaat ccaggacact
     7981 gtccaggaca agctcaaaaa tgtattgaat ttaagaaagc tggagcagaa gttatattaa
     8041 taggaaactg tactgactgt agtaacacag taatgggttc tgcaccaaaa ttaaaattag
     8101 gtgtatatca tattactgac catgtaatga gaacagttaa tcacccatta ataagaagaa
     8161 taaaaggtga cgtaagatta aaaataaaat aatagataaa tagaagttag acatctttag
     8221 aagtaaaact taaaagttac ttctaaggat gttttttaat gtattttgag ggcaatatat
     8281 atattgtaaa ataaatggaa aaaatagaca aaatatgcta gatattgtat aatgataaca
     8341 atagctatta caattaatat tggacaaaaa taatgatact caagtatgtg gtttgtatta
     8401 atatgcatat tatattaagt caactatccg atagagtaaa aggtttttgt gatatttgaa
     8461 gatactctat agtatctttt ttattataaa aactaatttt tgtcttttat tagataagta
     8521 tctaggatac taatttatat tttattgagt tgttaaaaaa gtgcacttaa aaagttaaaa
     8581 aagaaaaatt aaaaacctat ttatagaaac tgtgaagtat atcttaaacc tgggcactta
     8641 aaagatatat ggagttagta gtgcaacctg ctataaatat aaataggagt aacttttaat
     8701 tgtcaaaaat atataaaata aatacataaa taagtaatta atagaagata gatataaata
     8761 tatttttgaa atgctccctg cattcataac gagtgtagag tggcagatat ctgcccaaaa
     8821 ctcctttaat tgctggaagt tcctaaagct aactaaacca caacataatc atgaaataag
     8881 gataagtgtg atagagtgtg catttaataa gcacttgagg ttacgaaagt agaaaaaatt
     8941 agttagatag cataaggtta aatcctaagt gccttgaaaa tggataatca gcaggtaagc
     9001 ttcgaaaaga agaaacttca acgactagag agaaatctcg tacctacaag tgtggggaaa
     9061 tggggagtac ctaatttatg gttaggtaat gatatagtct gtgcttaata gaaatattaa
     9121 gaagttcata ggagaactgg ttaggagtaa cgaccctaat tgaacacact aaagatgtat
     9181 tattgaataa gattcatata atatttaata attttaatta tattaagtga atacaatata
     9241 tgtttttagt atcaggagaa ttttgaaaac aaaaattaaa aaatcaagta taatcagttt
     9301 agtaattgct tttagtatgg tatttactgc atttacacca atagtatctt atgctgacga
     9361 agtgactagc aatgatacaa tacttaatgg agaagaacaa tcaaatggtc agacatcaga
     9421 tgtagagaaa ccatcagacg gtcaggtacc agatggggag aaaccatcag acagtcaagt
     9481 accagatgta gagaaaccat cagacggtca ggtaccagat ggggagaaac catcagacag
     9541 tcaagtacca gatggggaga aaccatcaga cggtcaggta ccagatgggg agaaaccatc
     9601 agatggtcag ataccagatg gggagaaacc atcagatggt cagataccag atggagagaa
     9661 accatcagat ggtcagatac cagatgggga gaaaccatca gatggtcaga tgccagatgg
     9721 agagaagaca tcagatagtc agacgccaga tgtagagaaa ccatcagatg gtcagatgcc
     9781 agatggggag aaaccattag atgagcaaac tccagaagaa gaaaaaccat tggaagaaga
     9841 aatagttata gaggaaatga gtttaaaaca agatattgat aaaattcttg atatgacttt
     9901 atctcaaata aataaaatag tatataactt ttgggaagat gaagaagatg taaaggcaga
     9961 tgaacaatca gaaattaacc aagtttttac ttcagaagac tcatttatta gcttatggta
    10021 tgacaaaaaa gcaaaagtaa aaaattcttg tttactaaaa gaaatagatg gttcagatag
    10081 accttatcat gatttaagat ttgatgatat tacaaagact ttaacttttg actatgtaat
    10141 aaagggtctt gtaggagcaa gctcaaaaga cgacaaatat gtaatagaag gtgaagatgg
    10201 agagcaaact gcattttgtt acaataatca tttaagacca ccaagtagta atggaaagag
    10261 tccatattta ccagcagaag attttaatgg tcaagaaaac caaaatgaag aagcagttaa
    10321 atctatacta tatgcaggtt ctgaatttga tggatttgga tataagcaac aatttaattt
    10381 aggtggagaa gaaaatgaat tgatgactta ctcagctact caaagtgcaa tttggattat
    10441 tcttggacaa atggatgaac aagaaagact taagcagtat caaggttcta taaacctttg
    10501 tgataaactt atagaaagag caaaaactga agaggaaaga gctgagtatc agaagaagaa
    10561 agaagtagcg ataaatataa aagcttattt agaagcattg ttaaaagcag gtcgtgaaga
    10621 gttaaagcca aataatacag gaaaacctag tttgagtaat ggtttaacta agattaactt
    10681 tgaaaaaaat gaagatggta cttatgaaac agaagctgta gccttagtag gttatagtgg
    10741 tgtagtaaag ttaaaactac cagatggagt aactgcttat gatggagatg gtaatataat
    10801 aggtacaggc gaagtagaaa tttcaacgca acagaaattt aaacttaaga gtgttggaaa
    10861 accagatgcc aaagctaata tttctgctgt atcttatgat tatatttttc caaaagctat
    10921 tcaatattat aaggcagtgc ttgatttagg acaaaaagac cattcatctt cattaccatc
    10981 tagcaagcaa aatttattaa gttatacaat agagaaaaag aatggtcaag aagttaactt
    11041 taatataggt ttaccaacag atgatgatga tgttgtaaat ccaccagtgc caccaataga
    11101 tgatgatgtt gtaaatccac cggtgccacc aacagatgat acaaatggac ataagccaaa
    11161 accatcacca ccaatagatg atacagtaat aaatccacca gtaccaccaa tggatgatac
    11221 aataataaat ccaccagtac caccaacgga ggatgcagtg ttaaatccac cagtgccacc
    11281 aatggaggat gcagtgctaa atccaccagt gccaccaaca gaggatacag tgttaaatcc
    11341 accagtacca tcagtaagtg atacagtgga aaaaactcca gaactatcaa gagatgatac
    11401 aataataaaa tcaccaaaaa caggtgatga aactcagcta atgtcatatg tatttattag
    11461 tgtcattgca atttgtggat tagcgtatca atgtaaaata aaaagaaatt aatctagatt
    11521 attattaagt ttaaaataaa aagtctcaaa ttgatttata agtcattttg gggctttttt
    11581 atgtaaatat aatatgcaaa tatagtttgt ttaaaaatat ctattttaat tatattctta
    11641 aatttattat tggatacagg cattcattaa ttgtttgatg aatagaaaaa tgttttgaag
    11701 gtgaaaatag tattgaaaaa ataatatact aagggtataa ttcatttgat ttcatttgta
    11761 taaatttaat tataaatttt aaagtgatta tataaattta aatgctaatt acttaataaa
    11821 ttttaataat aattatttaa taaataatta ttataatatg ctagaataaa tatatataaa
    11881 taagttttga tgaggtacta ctataaaggg gggactttat gttttcgata ccagaagtaa
    11941 aaaaagtaga agaggtaatg gatacaaaat ttacaactat agacgaagac acaagaattg
    12001 aagatgcaat aaaagaaatg ataaaaagca acacaaaaac tctaatggta atagattcat
    12061 ctgaccaatt aaaaggcatt atatccatga cggatataca taatctttat gagatgcata
    12121 aaaagtatga aggtcaacca gtaaaactta taatgaagaa ggatgtaata tatgtcaacg
    12181 aaggtttaac cttagatgaa tgtagagata taatgatatt aaaaaatata gggattcttc
    12241 ctgttcttag agataataaa attattggag ttctaaaaca agagcacatt agggactatc
    12301 tatatatgca tttagaagat tatggattaa ctttgaagta tattataggt caaattaaag
    12361 aaggaatttg tgctataaat aacgaaggtg ttgttatctt gtggaataag tttatggagg
    12421 aaaggtatga tataaagtct gaagatatag ttggtcgacc gatgaatgaa tttttggaaa
    12481 atactatttc agaaaaagta ctaaatagta aagttggaat gagtgacttg tattttacag
    12541 ataaaaaaga aaatatgtat gcattagttc atgcaaatcc tatattttat aaagaagaat
    12601 ttatcggtgt tgtatgtaca gaagttgatg ttacagaagc taagatatta gctcttgaac
    12661 ttgaaaaagt aaatgataca ttaaaatatc taaagaacga agtcaaaaat ttatctaaag
    12721 gtagttttga taagatttta ggtaaaagtt ataaattgga aaaatcgaaa gcgattgcaa
    12781 aacaagtggc tagaacaaat agtagtatat ttatttgggg cgaaagtggt acaggtaaag
    12841 aagtatttgc aagagctatt catgattata gtgagagaaa agggcaattt atacctgtta
    12901 actgtagtgc cataccaaat gagttatttg aaagtgagtt ttttgggtat gaatcaggtg
    12961 catttacagg agcaagtaaa aagggtcgaa taggtatatt tgaacttgca aaagatggaa
    13021 ctgtattttt agatgaaatt gccgatttac ctcttagtat gcaagcaaag ttgcttagag
    13081 tattacagga aaaagaaata agaagagttg gtggagatac cacaataaaa ataaatccta
    13141 gaataatatc agctaccaat aaagatttga gtaaaatggt aaaggcagaa aaatttagag
    13201 aagatttata ctatagatta aatgttgttg aaataaagat tcctccactt agagagagaa
    13261 aagaagacat aggattattg gttcatagct ttttagagga gatatgtaaa caaaataata
    13321 agcctgttct tacaattagc aaagatgtaa tagatatatt tcagaattat agatggaaag
    13381 gtaatattag agaattaaaa aatacgattg aaaatattgt tgtattgtca caaaattcta
    13441 aaatagatgt ggatgatgta ccaagttata tgatggattc tacaaataat agtactgaag
    13501 aagaagagta tcctttagat ttaacgaagg caactcaaaa aattgaaata aagaatataa
    13561 ccaaggcact taaaatgtct aatggaaata aagctaaagc agctaagata ttaaatatac
    13621 ccagaacaac tttgtattat aagatagacc aatacaaaat agatgtgtct aaaatatgac
    13681 acatgactaa attttgacaa aatgataaac tttaatttaa aacctagtaa agccaacact
    13741 tgtataagtg ttgtgtatat agatgtgtta aaaaaaatga aaaagtgttg attaattgac
    13801 actttttttg tttgagaaaa tgtttacaaa tacaaaaaat aacatatttt aaataaatat
    13861 ttatataata tttggctaaa aatagcgata gatgaagaat ctattataga aaaaatcttt
    13921 ttgttaataa gttggcatag gaattgctta taatataaag tgactttaag tgagaaaaaa
    13981 ataatattaa tcataacaaa ttatgtaggt tttaatttac ttaatttctc agttaagggg
    14041 ttacctgttg aggaattgag atttaaaata ataaataaga aaataatgta aggaggtatt
    14101 ttatactatg tcaataactt tagaaacagc tcaagcccat gcaaatgacc cagcagtttg
    14161 ttgttgtaga tttgaagcgg gaacaattat agcgccagaa aacttagaag atccagcaat
    14221 atttgcagac ttagaggatt ctggattatt aacaatacca gaaaatggat taactatagg
    14281 tcaagtacta ggagctaagt taaaagaaac tttagatgca ctttctccaa tgactacaga
    14341 taacgtagaa ggatacaaag caggagaggc taaagaagaa gtagtagaag aaacagtaga
    14401 agaagcagct ccagtatcag aagcagcagt agttccagta agcacaggag ttgcaggtga
    14461 aacagttaaa atacacatag gtgaaggtaa gaacataagc ttagagatac ctttatcagt
    14521 agctggtcaa gcaggagttg ctgctccagt agcaaacgtt gctgctccag tggcaagtgc
    14581 agcagcagaa gtagctccaa aagttgaaga aaagaaactt ttaagaagct taactaaaaa
    14641 acactttaaa atagataaag ttgaatttgc tgatgaaact aaaatagaag gaactacttt
    14701 atacatcaga aacgcagaag aaatatgtaa agaagctaat gaaactcaag agttagttgt
    14761 agatatgaag ttagaaataa taactcctga taaatatgaa acttacagtg aagctgtatt
    14821 agatatacaa ccaatcgcta ctaaagaaga aggcgaatta ggttcaggta taactagagt
    14881 tatagatgga gctgtaatgg tattaactgg tacagatgaa gatggagttc aaataggtga
    14941 atttggttct tcagaaggtg agttaaatac tactataatg tggggtagac caggtgctgc
    15001 tgacaaaggt gaaatattca tcaaaggtca agtaacaata aaagcaggaa ctaacatgga
    15061 aagaccagga cctttagctg ctcaccgtgc atttgactat gtaactcaag aaataagaga
    15121 agcattaaag aaagttgaca actctttagt agttgatgaa gaagtaatag agcaatacag
    15181 aagagaaggt aaaaagaaag ttgttgttat aaaagaaata atgggacaag gtgcaatgca
    15241 tgataaccta atattaccag ttgagccagt tggtacatta ggagctcaac caaacgttga
    15301 cttaggaaac atgccagttg tattatctcc acttgaagta ttagatggtg gtatccatgc
    15361 attaacttgt ataggacctg catcaaaaga aatgtcaaga cattactgga gagagccatt
    15421 agtaataaga gctatggaag acgaagaaat agatttagta ggtgttgtat ttgttggttc
    15481 tccacaagta aatgctgaga aattctatgt atctaagaga ttaggtatgt tagttgaagc
    15541 tatggaagtt gatggagctg tagtaactac tgaaggtttc ggaaacaacc atatagattt
    15601 cgcatctcac atagagcaaa taggtatgag aggtatacca gtagttggtg taagtttctc
    15661 agctgttcaa ggtgctctag ttgttggtaa taaatacatg actcacatgg tagacaacaa
    15721 taagtctaag caaggtatag agaatgaaat attatctaac aacactttag ctccagaaga
    15781 tgctgttaga ataatggcta tgcttaaaaa tgctatagaa ggtgtagaag ttaaagctcc
    15841 tgaaagaaaa tggaatccaa atgttaaatt aaataacata gaagctatag aaaaagttac
    15901 aggagaaaaa atagtattag aagagaatga gcaatctcta ccaatgagta agaagagaag
    15961 agaaatatac gaaaaagacg aaaactaaaa tttagatact aactaagatt tagacaataa
    16021 cataaattta gatccaagtt taaaacagta tagagaggta gaattataat ggataggtta
    16081 aaatatatct catcagagac tttttatgaa ggtattatag ttgacatcaa aggcggtgga
    16141 gtgactatag acctaaaagg tagacttgga caatttaaaa tacctaatag aatgcttata
    16201 actgactacg aacttaagat aggtcaagaa gttggattca tgctaagcta tcctgaggta
    16261 ttaagtccag agcctaatca agagtatgta gaaaatatta ggagagaaaa agaaaaacaa
    16321 gcagaaatgc aatcaaaaca agaagaaaaa taataaataa tcgtttgaaa atagaaggga
    16381 gaggaaatat atgagcctta caacagtaca aggacttcaa tctgaaatat tcgttccaat
    16441 aacacctcct ccagtttgga ctcctgtaac taaagaatta aaagatatga ctatagcttt
    16501 agctacagca gctggtgttc atttaaaatc tgacaagaga tttaatctag caggagatac
    16561 tacatttaga gctataccta atacagctac agttgatgaa atgatggtat cacatggtgg
    16621 atatgacaat ggagatgtaa acaaagatat aaactgtatg ttccctatag atagattaca
    16681 tgaattagct gcagaaggat ttatcaaagg agtagctcct atgcactatg cattcatggg
    16741 tggtggagga aaccaacatg tcttcactga agaaactggt cctgctatcg ctgctaaact
    16801 taaggaagag ggagtagacg gtgtagttat gacagctggc tgaggtactt gccatagaac
    16861 tgccgtgatc gtgcagagag caatagagga agctggaata cctacaataa taatagcagc
    16921 tcttcctcca gtagttagac aaaacggaac tcctagagca gttgctccat tagttccaat
    16981 gggtgctaat gctggtgaac cacataatat agaaatgcaa acacatatat taagagatac
    17041 attagagcaa ttagttgcaa taccatctgc tggtaagata gttccattac catacgaata
    17101 taaagctcac gtttaaaatg taatttatat ttgaaaatat agaacccttc tcctacacgg
    17161 ttaggagagg ggttttaaga acaaaggtgg atattatgga agaaaaaata atgagaagac
    17221 ttgttataaa accgtttcat atgaatgaag ttaatttcgg ttcaaaaact tctataaaga
    17281 aagatgtact tacaatagat ttatcaagta tagatgaaat aaaagaaaga gaagagttaa
    17341 taacagacat aaaagtagac attataaaac ctggagatta tgacagagaa attaatacta
    17401 ttatggacat tataccaata tcaacaaaag ttttaggcag actgggagag ggtattacac
    17461 atactttaac gggtgtatat gtaatgttaa ctggtgcaga tgaagatgga aatcaaatgc
    17521 atgaatttgg ttcttcagaa ggaattttga aagaccaaat ggtatttggg agatatggaa
    17581 ctccatcagt tgaagattat ataatccatg tagatgtaac tttaaaggga ggtttacctt
    17641 ttgaaagaac acttccactt gcagcattca gagcatgtga tgattttata caagaaataa
    17701 gagcgtcttt gaaaatggaa gacggaagaa atgctactca agttcgtgaa tattttgaca
    17761 aagttcgacc aaatgcaaaa aaggttgtca tagtaaaaca aatagcaggt caaggtgcta
    17821 tgtatgacaa tcagttattc tcaaaggaac ctagtggatt tgaaggagga acatcaatta
    17881 tagatatggg aaatgtacct atgattatat ctcctaatga atacagagat ggtgccctta
    17941 gagctatgac ttaagagagg tggatattat gggaatagga ccttcaacaa aagaaacatc
    18001 tcttcatcac tttagagacc cacttttaga catagttgaa agtgataagg atgtagattt
    18061 acttggagta atagtagtag gaactccaga tggaaatgaa aataaaacct ttgtgggaca
    18121 aagaacggcc gcttggctgg aagccatgag agtagatgga gctatagttt catcagatgg
    18181 ttggggaaac tcacatgttg actacgctaa cacatttgag gaaataggaa aaagagatat
    18241 acctgtagtt ggagttacat ttaatgggac tcaagctaag tttgttgtaa gcaatcaata
    18301 tatggatacc atagttgata tgaacaaatc aaaagaaggt attgagacag aagtagttgg
    18361 agaaaacaac acaaatgaaa tagatgctaa aaaagctttg gcatttttaa aacttaaaat
    18421 gagaaaacat ggataaaaaa tttaagtaag ggaatgagtt ttgtgggaat ttctacatca
    18481 atgaaaaaca catcacttca tatatttaga gacccgttac tagatttgat agataatgat
    18541 tctgacatag acttgttagg agtggttgta gttgggacat cagagcacaa tgaatggaaa
    18601 acatttttag caactagact tggaagatgg attgaatcat tgcgacctga tggagtgatt
    18661 ataactttag atatggcagg aaaccagcac attgatttta caaatgctat agcagagttt
    18721 gtaaaatcag atatacctac agtaggtctt actattatgg gagcagatgg attagtaatt
    18781 accaatccat agttggataa agctaccata attgattata aaaagacaac aggctacata
    18841 gaaacagaag tagttggaga caatcacatg gatgaagttg atgttaaaaa agcattggct
    18901 ttcctaaaat taaaaatgcg taaagacgca aaataaacct aagggggaat ttgatatgaa
    18961 atttagcaga agtatacaag ctatagactc tcatacagca ggtgaagcaa ctagaatagt
    19021 tgtaggtgga atacctaaca taaaaggaaa ttctatgcct gagaaaaaag aatatttaga
    19081 ggaaaatcta gattatttaa gaactgctat aatgttagag ccaagaggac ataatgatat
    19141 gtttggttct gtaatgactc aaccttgttg cccagatgct gactttggaa taatcttcat
    19201 ggatggtggc ggatacctta acatgtgtgg ccatggtaca ataggagcta tgacagcagc
    19261 tatagaaaca ggtgtagttc cagcagtaga gcctgtaact catgtagtta tggaagctcc
    19321 agcaggaata ataagaggag acgttacagt tgtagatgga aaagctaaag aagtatcatt
    19381 cttaaacgta ccagctttct tatataaaga aggtgttgaa gttgacttac caggtgttgg
    19441 aactgttaaa tttgacatat catttggtgg aagcttcttt gctataatac atgcaagtca
    19501 attaggttta aaaatcgaac ctcaaaatgc tggtaaatta actgaactag ctatgaaact
    19561 tagagatata ataaatgaga aaatagaaat acaacatcca actttagctc atataaaaac
    19621 tgtagactta gttgaaatat atgatgagcc aactcatcca gaagctactt acaaaaatgt
    19681 agttatattt ggtcaaggtc aagttgacag atctccatgt ggaactggaa caagtgctaa
    19741 attagctact ttacatgcta aaggtgaatt aaaagtagga gaaaaattcg tatatgaaag
    19801 tatattagga actttattca aaggtgaaat agttgaagaa actaaagttg cagatttcaa
    19861 tgctgtagta cctaaaataa ctggttctgc ttatataact ggatttaatc attttgtaat
    19921 agatgaagaa gacccactta aacatggatt tattcttaaa taagtaataa ataaattagg
    19981 aaattaaaat agaatagtag ccacttgtcg gttgctattc tatagctttt aggctataag
    20041 attcagaata tatgaaaaaa taaaaaaata ggaggatgga ttatggtagc aatcttaaag
    20101 gcattatatg tagtattcgc agcaatcttt gcagttgtgt ttggtaaaga cgtagctaaa
    20161 gcaaacaaag aggggaaact agaagaacaa agtactccaa aagttgctgt aacaggattt
    20221 ataacagact tctttgatac attaggaata gggtcatttg ctccaacaat agcaatgtca
    20281 aaggctttaa agttaaatat accagataaa aaaatgcctg gtacattaaa cgtagcacat
    20341 actattccag ttgttacaga agcattcata tttacatcaa tcataccagt tgatggtgtt
    20401 actttagtat ctatggttgt ggcagcagca gttggttcat atataggtgc aggaataata
    20461 gctaagatgg acgagagaaa aatacaactt gttatgggtg taactttagc attaacagca
    20521 atattaatgt tattaggtca tccttggatt aacgtattac caggtggagg aaatgaatta
    20581 ggtcttactg gtgttaaatt agtaataggt gtaataggta actttatact aggagcttta
    20641 atgacagctg gtataggttt atatgctcca ggtatggcaa tggtttattt cttaggaatg
    20701 tctgcaaaag ttgcattccc aatagttatg ggttcttgtg cattattaat gccagttgca
    20761 agtatgaaat tcataaaaga agatgcatat actaaaaaag catcagttat aatagctata
    20821 tgtggtttaa taggagtttt cgtagctgca tatatagtaa aaactctacc aatggatata
    20881 ttaaaagcat tagtaatagt agttatagct tatacttctg ctacaatgat aatggctgca
    20941 aataaaaaca aaaagactgc ttagttaaag actacttagt gtttattaaa tattacagct
    21001 aataataaag attataaact gctttaaaat attgaattaa tattttaggg cagtttttta
    21061 tttactaaat taatataaag caattaacat gccaattatg taatatatat agtgatttag
    21121 tagatttaat gtaaaaaacg ttgtattttt aaagaataaa gcataattta aagctaaata
    21181 tttaaatttt ataatcaata agttcaataa atgttcatat attaatacag attaataaaa
    21241 atataaccta taataagtct aatataactt ataattaaga aaaataagtt tataatgact
    21301 caatattcat ctgaattatt tgaataaatt taattcgttg aagttgctta tatagagtga
    21361 a
//
LOCUS       DAMJWS010000075        21124 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMJWS010000075 DAMJWS010000000
VERSION     DAMJWS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.076"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..369
                     /gene="tuf"
                     /locus_tag="PC399_002795"
     CDS             <1..369
                     /gene="tuf"
                     /locus_tag="PC399_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2593644.1"
                     /translation="DNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEE
                     GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLR
                     FSIREGGRTVASGVVATIIE"
     gene            741..1052
                     /gene="rpsJ"
                     /locus_tag="PC399_002796"
     CDS             741..1052
                     /gene="rpsJ"
                     /locus_tag="PC399_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF2593645.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            1144..1773
                     /gene="rplC"
                     /locus_tag="PC399_002797"
     CDS             1144..1773
                     /gene="rplC"
                     /locus_tag="PC399_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF2593646.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1803..2426
                     /gene="rplD"
                     /locus_tag="PC399_002798"
     CDS             1803..2426
                     /gene="rplD"
                     /locus_tag="PC399_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF2593647.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2426..2716
                     /gene="rplW"
                     /locus_tag="PC399_002799"
     CDS             2426..2716
                     /gene="rplW"
                     /locus_tag="PC399_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF2593648.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2746..3576
                     /gene="rplB"
                     /locus_tag="PC399_002800"
     CDS             2746..3576
                     /gene="rplB"
                     /locus_tag="PC399_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF2593649.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3611..3892
                     /gene="rpsS"
                     /locus_tag="PC399_002801"
     CDS             3611..3892
                     /gene="rpsS"
                     /locus_tag="PC399_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF2593650.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3924..4259
                     /gene="rplV"
                     /locus_tag="PC399_002802"
     CDS             3924..4259
                     /gene="rplV"
                     /locus_tag="PC399_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF2593651.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            4282..5097
                     /gene="rpsC"
                     /locus_tag="PC399_002803"
     CDS             4282..5097
                     /gene="rpsC"
                     /locus_tag="PC399_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF2593652.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            5133..5564
                     /gene="rplP"
                     /locus_tag="PC399_002804"
     CDS             5133..5564
                     /gene="rplP"
                     /locus_tag="PC399_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF2593653.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5566..5769
                     /gene="rpmC"
                     /locus_tag="PC399_002805"
     CDS             5566..5769
                     /gene="rpmC"
                     /locus_tag="PC399_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF2593654.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5793..6047
                     /gene="rpsQ"
                     /locus_tag="PC399_002806"
     CDS             5793..6047
                     /gene="rpsQ"
                     /locus_tag="PC399_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF2593655.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            6073..6441
                     /gene="rplN"
                     /locus_tag="PC399_002807"
     CDS             6073..6441
                     /gene="rplN"
                     /locus_tag="PC399_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF2593656.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6465..6770
                     /gene="rplX"
                     /locus_tag="PC399_002808"
     CDS             6465..6770
                     /gene="rplX"
                     /locus_tag="PC399_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF2593657.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6800..7342
                     /gene="rplE"
                     /locus_tag="PC399_002809"
     CDS             6800..7342
                     /gene="rplE"
                     /locus_tag="PC399_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF2593658.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            7360..7545
                     /locus_tag="PC399_002810"
     CDS             7360..7545
                     /locus_tag="PC399_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF2593659.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7578..7976
                     /gene="rpsH"
                     /locus_tag="PC399_002811"
     CDS             7578..7976
                     /gene="rpsH"
                     /locus_tag="PC399_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF2593660.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            8007..8549
                     /gene="rplF"
                     /locus_tag="PC399_002812"
     CDS             8007..8549
                     /gene="rplF"
                     /locus_tag="PC399_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF2593661.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8585..8953
                     /gene="rplR"
                     /locus_tag="PC399_002813"
     CDS             8585..8953
                     /gene="rplR"
                     /locus_tag="PC399_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF2593662.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8974..9483
                     /gene="rpsE"
                     /locus_tag="PC399_002814"
     CDS             8974..9483
                     /gene="rpsE"
                     /locus_tag="PC399_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF2593663.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9498..9683
                     /gene="rpmD"
                     /locus_tag="PC399_002815"
     CDS             9498..9683
                     /gene="rpmD"
                     /locus_tag="PC399_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF2593664.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9716..10159
                     /gene="rplO"
                     /locus_tag="PC399_002816"
     CDS             9716..10159
                     /gene="rplO"
                     /locus_tag="PC399_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF2593665.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            10203..11471
                     /gene="secY"
                     /locus_tag="PC399_002817"
     CDS             10203..11471
                     /gene="secY"
                     /locus_tag="PC399_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF2593666.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11488..12138
                     /locus_tag="PC399_002818"
     CDS             11488..12138
                     /locus_tag="PC399_002818"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF2593667.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            12139..12885
                     /gene="map"
                     /locus_tag="PC399_002819"
     CDS             12139..12885
                     /gene="map"
                     /locus_tag="PC399_002819"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF2593668.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12907..13176
                     /locus_tag="PC399_002820"
     CDS             12907..13176
                     /locus_tag="PC399_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF2593669.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            13214..13432
                     /gene="infA"
                     /locus_tag="PC399_002821"
     CDS             13214..13432
                     /gene="infA"
                     /locus_tag="PC399_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF2593670.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13468..13581
                     /gene="rpmJ"
                     /locus_tag="PC399_002822"
     CDS             13468..13581
                     /gene="rpmJ"
                     /locus_tag="PC399_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF2593671.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13734..14105
                     /gene="rpsM"
                     /locus_tag="PC399_002823"
     CDS             13734..14105
                     /gene="rpsM"
                     /locus_tag="PC399_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF2593672.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            14141..14539
                     /gene="rpsK"
                     /locus_tag="PC399_002824"
     CDS             14141..14539
                     /gene="rpsK"
                     /locus_tag="PC399_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF2593673.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14571..15194
                     /gene="rpsD"
                     /locus_tag="PC399_002825"
     CDS             14571..15194
                     /gene="rpsD"
                     /locus_tag="PC399_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF2593674.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            15272..16219
                     /locus_tag="PC399_002826"
     CDS             15272..16219
                     /locus_tag="PC399_002826"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF2593675.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            16240..16581
                     /gene="rplQ"
                     /locus_tag="PC399_002827"
     CDS             16240..16581
                     /gene="rplQ"
                     /locus_tag="PC399_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF2593676.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16701..17534
                     /locus_tag="PC399_002828"
     CDS             16701..17534
                     /locus_tag="PC399_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2593677.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17522..18388
                     /locus_tag="PC399_002829"
     CDS             17522..18388
                     /locus_tag="PC399_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2593678.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18382..19185
                     /locus_tag="PC399_002830"
     CDS             18382..19185
                     /locus_tag="PC399_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF2593679.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            19217..19948
                     /gene="truA"
                     /locus_tag="PC399_002831"
     CDS             19217..19948
                     /gene="truA"
                     /locus_tag="PC399_002831"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2593680.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            20068..20499
                     /gene="rplM"
                     /locus_tag="PC399_002832"
     CDS             20068..20499
                     /gene="rplM"
                     /locus_tag="PC399_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF2593681.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20528..20920
                     /gene="rpsI"
                     /locus_tag="PC399_002833"
     CDS             20528..20920
                     /gene="rpsI"
                     /locus_tag="PC399_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF2593682.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8444 a         2495 c         4411 g         5774 t
ORIGIN      
        1 gataatatag gagcattatt aagaggagta caaagaaacg agatagaaag aggacaagta
       61 ctagcaaaga ctggatcagt aaaggcacac acaaagttta cagcagaagt atatgtactt
      121 aaaaaagaag aaggtggaag acatacacca ttctttgatg gatatagacc acaattctat
      181 ttcagaacaa cagacgtaac aggagcttgt aagttaccag aaggaataga gatggtaatg
      241 cctggagata acgtaacaat ggaagtagac ttaataaact caatagttgt agaagaggga
      301 ttaagattct caataagaga aggtggaaga acagtagctt caggagttgt tgctacaata
      361 atagagtaat cctttactaa actaatggat ttaatataaa aaaagagggg ggtaactcct
      421 ctcttttttg ttttttgttg acaagtagcg ctaaagcata tatactaaac tagtgccttg
      481 agacagatta acaatcaagt ttaaggttat gatgttgtaa tagaaaataa aattgaaaaa
      541 aacagaaaaa aaacttgaaa aaatgtaaca gatgatataa aatataatag tgtgcttgag
      601 agattaagaa aataataatt aaatcctagt gaaatacgaa ctgatatcga tttaaaagtt
      661 acaacacacc cggagcagtg tatcggtatt ggttgtacgc aacttgaaaa ttaaacacgt
      721 gataaaggag ggaaaacaaa atggctaaga atgaaaaaat aagaataaga ttaaaatcat
      781 atgatcacaa attattagat ttttcagcag gtaaaatagt tgaaactgct aaaaaggctg
      841 gatcacaagt ttcaggacct gtgcctctac caacagaaaa acaagttgta actatattaa
      901 gagctgtaca taagtacaaa gactctagag aacaatttga aataagaact cataaaagac
      961 taatcgacat agctaatcca acacctaaga cagttgactc attaatgaga ttagacttac
     1021 cagcaggtgt agatatagaa ataaaattat aagaacccct aagatattat cctatagatg
     1081 attatcttag aatgattact ctaaaagatg taatccgctg taagaattat aggaggtgct
     1141 aatatgaaag gaatattagg caaaaaagtt ggtatgactc aaatattcac tgataaaggt
     1201 gtagttatac ctgtaacagc tgttgaagca gggcctatgg tcgttactca aataaaaaca
     1261 gttgataaag atggatacaa tgctatacaa ataggttttg aagatgctaa agaaaaagct
     1321 ttaaacaaac ctaaaaaagg acatttagct gctgctaatg ttttaaagaa acatttaaaa
     1381 gaatttagag tagattctgt agaaggatac acagtaggac aagaaataaa agctgatgta
     1441 tttgaagcag gtgcaaaaat agatgttact ggaataagta aaggtaaggg attccaaggt
     1501 ccaataaaaa gacatggaca aagtagaggt cctgaaactc acggttctag ataccacaga
     1561 agaccaggtt caatgggagc ttgttcttac ccaggtagag tatttaaaaa caaaaaatta
     1621 gctggacata tgggaagcgt aaaggtaaca gttcaaaact tagaggttgt taaagtagat
     1681 gctgacaaga accttatatt agttaaagga gctataccag gagctaaagg ttcagtagta
     1741 actataaaag aagctataaa ggtttctaaa taatgactaa cctagagaaa ggaggaataa
     1801 caatgccaaa attaaatgta ctaaatgtta gtggacaaaa cgttggagaa atagaattat
     1861 cagattctat atttggcgta gaagttaatg gacatgtttt atacgaagtt gtaaaaaatc
     1921 aattagcaaa taagagacaa ggaactcaat ctgctaaaac tagagcagaa gttagaggtg
     1981 gcggaagaaa accttggaaa caaaaaggaa caggtagagc aagacaaggt tctacaagat
     2041 ctgtacaatg ggtaggtgga ggagttgctt ttgcacctaa accaagaagc tacaaatata
     2101 cattacctaa gaaggtaaga agattagcta tgaagagtgc tttatcttca aaagttcaaa
     2161 atagtgaagt tatagtatta gatgcattaa acatggatgc tcctaagact aaagaatttg
     2221 ctcaaatatt aaacaatata aatgctgcta aaaaagcttt agtagttata gctgacaaaa
     2281 acgataacgt aataaaatca gctagaaata tagaaggtgt tcaaactgca ttagttaata
     2341 ctatgaatgt atatgatata ttaaaatacg attcatttat aataactaca gatgctgtta
     2401 aaaaagtgga ggaggtgtac gcataatgac taatccacat gatgtaataa taagaccagt
     2461 tgtaactgag cacagtatgg ctgaaatggg tgaaaaaaaa tacacttttg ttgtggctaa
     2521 agatgcgaac aaaactgaaa taaaaaaagc agtagaaaaa gtattcggag ttagtgttga
     2581 taaagtaaat actttaaact acgatggaaa agttaaaaga atgggtagaa ctcaaggaag
     2641 aacatcaagc tttaagaaag cagtagttaa attaactgct gatagtaaag aaatagaatt
     2701 cttccaagga atgtagtatt ataacaaacg aaggagggaa aacagatggc tataaaaaag
     2761 tttagaccaa cttctcctgc cttaagacaa atgacagttt tagtttctga tgaaataaca
     2821 tgtaatcaac cagaaaaatc tcttttagtt aacttaaaga aaaatgctgg tagaaacgta
     2881 catggtagaa taactgttcg tcatagaggt ggaggacaaa aaagaaaata tagaataata
     2941 gattttaaaa gagataaaga tggtatacct gctaaggttg ctactataga atatgatcca
     3001 aacagaactg caaatatcgc attattaaac tatgctgatg gagaaaaaag atatatatta
     3061 gctccagttg gaattaatgt tggagatact atattatcag gattaggtgc tgatataaaa
     3121 ccaggaaact gtttagcatt aaaagatatg ccagttggta ctatcattca taatatagaa
     3181 ttaaagccag gaaaaggagc tcaattagtt agatctgctg gagtttctgc acaattaatg
     3241 gctaaagaag gaaaaaatgc tttattaaga ttaccatcag gtgagatgag attagtaagc
     3301 ataaactgta aagctactat aggtcaagtt ggaaatatag agcatggtaa cgtagttatc
     3361 ggtaaagcag gtagaaaaag acatatggga ataagaccta ctgtaagagg ttctgtaatg
     3421 aaccctaatg accatccaca cggtggaggg gaaggtagat ctccaatagg tagaccatct
     3481 cctgttactc catggggtaa accagctctt ggatacaaaa ctagaaagaa aaataaagct
     3541 tcaaataaac taatagtatc aagaagaact aagtagtaaa tttatttttg ctcggaaagg
     3601 aggaatataa atgtcaagat caactaaaaa agggcctttt gtccatgcaa gacttttaaa
     3661 gaagatagaa gctatgaatg ctagtggaaa taaagaagtt ataaaaactt ggtcaagatc
     3721 ttcaactgta tttccacaaa tggtagaaaa cactatagct gttcatgatg gaagaaaaca
     3781 tgttccagta tatataacag aagatatggt tggacataaa ttaggtgagt tcgttcctac
     3841 tagaactttc aaaggacata aggatgacga aaaatctaat aagagaaaat aattaaatta
     3901 tgactaagaa aggaggaata acaatggaag caaaagctac tgctaaatac gtacgtgtat
     3961 caccaagaaa agcaggccaa atatgtgacc ttgttagagg aaagaatgtt gatgaagcat
     4021 tagcaatatt aaagtttact ccaagaggag cagcttcaat aatagctaag gttgtaaaat
     4081 cagctaaagc taacgcagaa aacaatcacg aaatggatac tgaaaaatta tatatagcat
     4141 caatagttgc taatcaagga ccaacaatga agagattcat gcctagagct atgggtcgtg
     4201 caactacaat aagaaaaaga acttctcata tagaggttgt tgttaaagaa aaaaaataat
     4261 taatagatag gagggaaagt aatgggtcaa aaggttaatc cacacggctt aagggtcggt
     4321 gttataaaag attgggactc aagatggttc gctactgata aaaaagagtt cggaaactta
     4381 ttattagaag accataatat acgtaaattc ttaaagaaaa gattatactc agctggagtt
     4441 gctaagatag aaatagaaag atcagctaac aaaataaaaa tggacttaca cgttgctaag
     4501 cctggtgtag ttataggaag agctggtgca ggaatcgaag cattaaaagc tgaattagaa
     4561 aaaatgacaa agaaaactat aatagttaat atagtagaag taagaagtac agataaaaat
     4621 gctcaattag tagctgaaaa tatagcttta gctatagaga gaagggttgc tttcagaaga
     4681 gcaatgaaac aagctataca aagagcaatg aaatctggtg ctaaagggat aaaagtttct
     4741 gcttcaggaa gattaggtgg agctgaaatg gctagaactg aaggttatag tgaaggaaat
     4801 gtgccactac aaactttaag agcagatata gattatggtt tcgctgaagc tgatacaact
     4861 tatggaaaaa tcggtataaa agtttggata tgtaatggtg aagttttacc aactagagac
     4921 ggtgtaaatc caagagaaga aagcagaaag agcgatagaa gagacaacaa gagagataat
     4981 agaagaaacg atagaagagg aaatgacaga agaggaaatg ataacagagg aaactacaga
     5041 ggacaaagac ctcaaggtgg atcaagacct caaagaactg aaaacaaagg aaattaataa
     5101 tagttaggtt caaggaagga ggaaaaatac tcatgttaat gccaaaaaga gtaaagcgtc
     5161 gtagagttca tagaggaagc atggctgggc aagctcataa gggtaataaa gttacttatg
     5221 gtgagtttgg attagtagca ttagaagctt cttggataac ttctaatcaa atagaagctg
     5281 ccagaatcgc gatgactaga tatataaaaa gagggggaaa ggtttggata aaaatattcc
     5341 ctcataaacc agtaacaaga aaaccagcag agactcgtat gggtgcaggg aaaggttctc
     5401 cagaatattg ggtagctgta gttaagccag gaagagttat gtttgaatta gcaggtgttt
     5461 ctgaagataa agcaagagaa gctatgagac ttgctgcaca taaattacca atcaaatgta
     5521 aatttgttaa aaaagaagat ttagaagtaa agggtggtga ataggatgaa agctaaagaa
     5581 ttaagagatt taacaagcga agagctaatg aacaaattaa acgacttcaa aagtgaatta
     5641 tttagcttaa gattccaatt agctactggt caattagaaa atacagctag aataaagttt
     5701 gttaagaagg atatagcaaa agttaaaact gttcttgctg aaagaaagtt atacgaaact
     5761 agagcttaat ttggaaagga ggctttttaa acatggaaag aggaagaaga aaagttagaa
     5821 taggccgtgt tgttagtgat aagatggata aaacaatagt agtagctgtt gaagaattcg
     5881 tacgtcatcc actatataat aaacgtgtta agagaactaa gaaattcaag gctcatgatg
     5941 aaaagaatat atgtaacatc ggagatagag ttaaaataat ggaaactaga ccattatcta
     6001 aagataagag attcagacta gttgaagttg ttgagaaagt taagtagttt ttgaagaagg
     6061 agggattacg atatgataca acaagaatca cgtctaagag ttgctgataa ctcaggagct
     6121 aaagaacttt tatgtatacg tgttctaggc ggaagtaaaa gaagatatgg taacataggc
     6181 gacgttatag ttgctactgt taaaagtgca acacctggtg gagttgtaaa aaaaggtaag
     6241 gttgttaaag ctgttatagt aagaagcaaa caaggcgtaa gacgtaatga cggaagctat
     6301 atatcttttg atgaaaatgc tgctgttata ataaaggacg ataaaactcc agtaggaact
     6361 cgtatattcg ggcctgttgc tagagagtta agagacaatg aattcatgaa aatagtttct
     6421 cttgctccag aagtactata attaaggagg tgcaatagga catgatgcgt gttaaaaaag
     6481 gtgacactgt tgtagttata gcaggtaaag ataaaggtaa aaaaggttca gttttaaagg
     6541 tatatcctaa aactagcaaa gtgttagttg aaggtgtaaa tgtaataact aaacatcaaa
     6601 aaccaagtgc tatgaaccaa caaggtggaa taataaataa agaagcccca atacacatat
     6661 ctaatgtaat gccatttgat cctgaaacag gaaaaggcgt tagagtaaga tatgaagtaa
     6721 aagatgggaa caaagtaaga gtatcagcaa agagcggaaa agaattataa taaatattgc
     6781 ttgaaaggag ggaccttaaa tggcttctag attacaagaa aaatacatga aagaagttgc
     6841 tcctgcttta atggagaaat ttggatacaa aaacgtaatg gagataccta agttaaataa
     6901 aatagttatt aacatgggta taggtgacgc tagagaaaat ccaaaaggat tagaaaaagg
     6961 cgttgaagaa ttagaaatga tatcaggaca aaagcctgtt ataactaaag ctagaaaatc
     7021 tgttgctaac ttcaaattaa gagaaggaat gccaatagga acaaaagtta cattaagagc
     7081 tgacaaaatg ttctacttca tggacaaatt agtttcagtt tctttaccaa gagttagaga
     7141 ctttagagga gttaacccta atgcttttga tggtagagga aattatgctt taggagttaa
     7201 agaacaatta atattccctg aaatagaata tgataaaata gataaagtaa gaggaatgga
     7261 tataatattt gttactacag caaaaactga cgaagaagct cgtgaattat taaaattatt
     7321 aggaatgcca ttttctaagt aagcaaagga gggattcccg tggctagaaa agcgatggtt
     7381 gttaagcaac aaagaaagca aaagtacgct actagagaat acactagatg tactatatgc
     7441 ggaagaccgc attcagtttt gaaaaaattt ggtatatgcc gtatatgctt tagagaatta
     7501 gcttataaag gtcaaatacc tggtgttaga aaagcaagct ggtagaagct tgattagaat
     7561 ggaaggaggg caacaaaatg acaatgacag atccaattgc agatatgctt actcgtataa
     7621 gaaatgctaa tgttgttaag catgaaactg tagatgttcc agcttctaat atgaagaaag
     7681 aattagctag aatcttatta gaagaaggtt tcataagagg ttatgatgtt atagaagatg
     7741 gaaaacaagg tatcataaga atacaattaa agtacggaca agaaggcgaa agagttataa
     7801 caggtcttaa gaaaatatct aagcctggaa tgagagttta cgctgctaac catgagatac
     7861 caaaagtatt aaacggatta ggaatatcag ttatatctac ttcaaaagga atattaactg
     7921 acaagcaagc tagaaaagaa aatgttggtg gagaagtaat ctgctacgtt tggtaattaa
     7981 tatgaacaat gtaaggaggt gcgaccatgt caagaatagg tgttaagcca ataataatac
     8041 cagcaggtgt tgaagttact atagctgaag gaaatttagt tacagtaaaa ggtccaaaag
     8101 gaactttaac taaacaatta agtgctgagt taaacataaa aaaagaagaa aatacaataa
     8161 tggttgaaag accaactgat aataaaaaac atagatcttt acacggacta actagaactt
     8221 tattagataa tatggttgta ggtgtaaata ctggtttcga aaagaaatta gaattaaaag
     8281 gtgttggata tagagctcaa aaacaaggaa agaaattagt tatgaactta ggtttctctc
     8341 atccagttga gatggaagat ccagaaggaa taactgttga agctccaaac caaacagagt
     8401 taatagtaaa aggtatagac aagcaattag tagggaatta tgctgctaag ataagagctt
     8461 ggagaaagcc agagccatac aaaggtaaag gtataaaata cgttgatgaa gtaatcagac
     8521 gtaaagaagg aaaaactggt aaaaaataat accaaaactt aaacgctaga aaggagtgag
     8581 ctaggtgtta aaaaaggctg ataaaaatgc taatagactt caaagacata agagagttcg
     8641 tagaaaaata agcggtactt ctcaaagacc aagattatgt gtatttagaa gtgctaacaa
     8701 tatatatgct caaataatag atgacacaaa aagagtaact ctagttgctg catcttcttt
     8761 agaagcagaa gttaagagtg ctgtcaatca tacaggaaac aaggaagcag ctaagaaagt
     8821 tggagaatta gttgctaaaa aagctgttga aaaaggaatc actgaagttg tatttgacag
     8881 aggtggatac ttatatcatg gaagaattca agaattagct gaaggtgcta gagaagctgg
     8941 tcttaagttc taatacgaag gagggaaaaa tacatgctac gtcgtaagcc gatagatgca
     9001 ggacaacttg acttacaaga aaaagttgtt gaagtaagac gtgttactaa ggttgtaaaa
     9061 ggtggtagaa atttcagatt tgcagcatta gtagttgttg gagatgaaaa cggacacgtt
     9121 ggtataggtg ctgggaaagc aatggaagta ccagacgcta taaaaaaagc agttgaagat
     9181 gctaagaaaa atctaatagt agtgccaata gttgggacta caattcctca cgaagtacgc
     9241 ggacactttg gtgctggaaa tatattaata atgcctgctg tagaaggtac aggagtaata
     9301 gctgggggac ctgctagagc cgtacttgag ctagctggac taaaagacgt tagagctaaa
     9361 tctttagggt ctaacaatcc aagaaatatg gtaaatgcta caatagaagg acttaattct
     9421 ctaaaaacag ttgaagatat agctaaactt agaggtaaaa aagttgaaga acttctaggg
     9481 taaggaggta gtgtaaaatg gctaaattac aaataaagtt agttagaagt gttataggaa
     9541 ctactcctaa ccaaaaaaag aatgttgaag cgttaggatt aagaaaaaga gaacaagttg
     9601 ttgtaaaaga agacaatgct caaacaagag gtatgataaa taaagtaagt cacttattag
     9661 aagtaactga aatagctgaa taatttttat aaagtagtgc aaggaggtgc aatccatgaa
     9721 gttacatgag ttaaaacctg ctgaaggtgc agtaagagct aagagaagat taggtagagg
     9781 tactgcaact ggacaaggta aaactgcagg acgtggacaa aaaggtcaat ggtctcgttc
     9841 tggtggtgga gtaagagtag gatttgaagg tggacaaatg ccactagcta gaagacttcc
     9901 taagagaggt ttcaataaca tatttaagaa agtttacaca gaagttaatg ttgaagtttt
     9961 aaacagattt gaaaatggaa cagaaataac tgcagaatta ttaaaatcta ctaaaactat
    10021 aagcaaaata ggtaaagacg gaattaaaat cttaggtgaa ggtaatttag agaaagcttt
    10081 aactgttaaa gctgctaaat ttacagcttc agctcaagaa aaaatagaaa aagctggtgg
    10141 aaaagcagag ttagtataat aacctgctaa tctcctagcc agtacaggcg gggtgaatta
    10201 gcgtgctgtc aaaattaaaa caagcttgga agattaaagc agtaagaaaa aaagtaatgt
    10261 atacacttat gatgatcgtg atattcagaa taggtactac tataccagtt ccaggcatag
    10321 acactagtat tatacaaaag atggtaggcg gcaatagtct actttctctg tataatatgt
    10381 ttactggtgg agcatttagt aacttttcac tatttgcttt aggtataagt ccatatatca
    10441 ctgcatcaat cataatacaa cttctaacag taggttttga aagtttagca gaacttcaaa
    10501 aatctggtga agaaggaaag aaaaaaatca ataagtacac taaatataca gcactagctc
    10561 tagcagttgt acaagctttg ggaattacat taggaattgt aagaagtgct ttaatatcaa
    10621 atagtgtgtt ttttatcaca actgttgtaa taacattagt ttcagcaagt atgttagtaa
    10681 tgtggattgg ggacaaaatt actgaaaaag gaatcggtaa tggtagttca gtaataatat
    10741 ttgcaggtat aatatcaaga atacctacag atgttattaa aatatcgcaa caagttaaat
    10801 ctggcgaagt tgcaccttgg gttatagtga ttctagctgt agttatatta cttacagtaa
    10861 caggagttac atttatacaa gaagcaacta gaaagatacc tgtacaatat gctaaaagag
    10921 ttgttggaag aaaaatgtat ggaggacaaa gttctcatat accaatgaaa gttaaccagt
    10981 caggagttat gcctataatt tttgctagtt cacttttagc tttccctcaa acaatagcta
    11041 tgtttatggg tccaaatgct caggcttttg ttcaaaaata cttaagtatg gcaacagaac
    11101 aaggattttg gacatataga tcaattgaga ttctacttat aattttcttc tcatattttt
    11161 atacaactgt atcatttaat acagaagaca tatctaaaaa tatgaaaaat aatggtggat
    11221 ttataccagg tataagacca ggtgagccta ctatggatta tttaaataga attttaacta
    11281 gattaacttt agctggagct actttcttag caataattgc tatggtgcca gctcttacaa
    11341 cacattatat gaaagtaaat atgagtctag ctgggacttc attgctaatc gttgttggag
    11401 ttgctttgga gcttaagaga cagttagagt caaacttagt tatgagaagc tatcaaggtt
    11461 tcttaaaata aaatggagat ggataatatg agaataatat tacttggacc tccaggtgct
    11521 ggtaaaggta ctcaggcggc aggaatagta gaaaaataca atatacctca tatatcaaca
    11581 ggagatatat tcagaaagaa tataaaagag ggaacagaac ttggaaaaaa agctaaagaa
    11641 tacatggacc aaggtttatt agtaccagat gagttaactg taggtttagt tactgataga
    11701 atatctcaag aagattgtaa aaatggattt atgttagatg gatttccaag aaatgtagca
    11761 caaggagaac atttagatat cttcttaaaa aatgctggta tatcactaga taaagttgtc
    11821 aatattgaag ttgataagag tatattagtg tctagagcag ttggtagaag aatatgtaag
    11881 tcttgtggag ctacttacca tgttgagttt aatcctccta aagtagaagg tgtatgtgat
    11941 gtatgccaag gagaattata tcaaagagct gatgataatg aagaaactgt atctaagaga
    12001 atacaagttt atctagatga aactaagcct ttagtagatt attatagcaa acaaggtata
    12061 atagcagata taaaaggtga tcaagcaata gataaagtat ttgaagatat tgtcgcagcc
    12121 ttaggaagtg gaaaataaat gattatcttg aaatcaaaaa aagaaataga acttttaaga
    12181 gaagcaggta aaatagttgc tgacactcat gaagttttaa gaaaagctat ttctccggga
    12241 atatctactt tagaattaga taaaatagct gaagagaata taagaaaata caatgcagaa
    12301 ccttctttta aaggatatgg aggttttcca ggaagtatat gtgcttctat caatagagag
    12361 gtagtacatg gtattccagg agaaactata ttacaagaag gtgacattgt aagtttggat
    12421 ataggagcct attataaagg atatcatggt gattctgcga aaactcacgg agtaggcatg
    12481 atatctgaag aagataggaa attgatagaa gtaactagag aaagcttcta tgaaggaata
    12541 aaatttgcta aattaggata tagactttca gatatctcac acgcagtaca aacacacgtt
    12601 gagaaacatg gtttctcggt agttagagat ttagtgggtc atggagttgg agcaaatctt
    12661 catgaagacc cacaagtacc taattatgga ctcccaggaa aaggaccaag acttagagaa
    12721 ggaatggtta ttgctataga gccaatggtt aatgcaggtc gttatcatgt aaagactcta
    12781 tcagatgggt ggacgactgt tacaatagat ggaaaaaaat cagctcatta tgagcatacg
    12841 atagcaatta ctgaacatga acctttgata ttgacaaagt tgtaatttaa aaagtaggtg
    12901 aaaaaagtgc tatcagataa tttaagtata ggtcaagttg ttaaagtttc attaggacga
    12961 gataaaggaa atttattctt tgtagtcaaa ataattaata atgaatatgt attaatagct
    13021 gatggaaaaa agagaaaact tgacaaacct aagttaaaga aagtgaagca cttaaaaaaa
    13081 tataatttta ttaatgatga agttagaaaa agagtagtat ctggacaaga gattacagat
    13141 tcttttttaa gagctgaact tactaagtta aattagataa tttaacttat tgaaatggag
    13201 ggctggttag ttaatggcca aaaaagatgt tatagaatta gaaggtacag tttcagaagc
    13261 tttacctaat gctatgttca aagttaaact agaaaatgga catgagatat tatgccacat
    13321 ttctggaaag ctaagaatga acttcataag aattcttgaa ggtgacaagg tgaatgttga
    13381 actttctcca tatgacctta caagaggaag aattacttgg cgtaagaagt agactatatt
    13441 aatatttagt ctaaggaggg attaataatg aaggttagac catcagtaaa accaatatgt
    13501 gaaaaatgta aagtaataaa aagaaaagga aaagtaatgg ttatctgtga aaatccaaag
    13561 cataagcaaa agcaaggata agaaattatt actagcattt tgttaaattt tatagtataa
    13621 tactatattg tgatgttaac aaaagaacgt taaaatcgtt gaacaatctg gcgtaggatt
    13681 ggcatatgat gtgaagtttt aaacactaaa aactatgtgt aggaggtgcg atcatggcaa
    13741 gaatagctgg ggtagattta cctagagaaa aaagagcgga gataggctta acttatatat
    13801 atggtatagg taaagcaact gctaacgaaa tattagctaa ggctgagata aatccagata
    13861 ctagaataaa agatttatca gaagatcaag ttaatgagtt aagaaaagtt atagatgacg
    13921 atttcttagt tgaaggtgac ttaagaagag aaattgcttt aaacataaag agattaagag
    13981 atataaaatg ttatagagga ataagacacg ctaaaggtct tccattaaga ggacaaagaa
    14041 ctaagactaa tgctagaact agaaaaggtc ctagaaaaac agtatctcgt aaaaagaaaa
    14101 aataaataaa taataactag atgaaggagg gaaaaggaaa atggctaaac caaaaaagaa
    14161 agttacacgt attagaagaa gagaacgtaa aaatatagaa cgtggtcatg ctcatataca
    14221 atcaactttc aataatacaa taataacttt aactgacgtt cacggaaatg ctatatcttg
    14281 ggcaagttct ggacaattag gattcaaagg atcaagaaaa tcaactccat ttgcatctca
    14341 aatggctgct gaaacagctg caaaagctgc gatggaacac ggactaaaaa gtgttgaggt
    14401 attcgtaaag gggccaggtt caggaagaga agctgcaata agagctttac aagcaactgg
    14461 actagaagta actatgataa aagacgttac tccaatccca cataacggat gcagaccacc
    14521 aaagagaaga agagtgtaat taataagagt acaataggag gtgtagataa atggcaagat
    14581 atacaggtgc atcatgtaga caatgccgta gagagggaat gaaattattc cttaaaggtg
    14641 atagatgtta tacagacaaa tgtgctatag taaaaagaaa ctatgctcca ggtcaacatg
    14701 gccaaggaag aaagaaagtt tctaactatg gattacaatt aagagaaaaa caaaaagtta
    14761 aaagaatata tggagtttta gagactcaat ttagaaattt atatgaacgt gctgaaaata
    14821 tgcctggtaa agcaggtgag aacttattaa gtttattaga gagaagatta gataatgtag
    14881 tttacagaat gggactagca tcttctagaa aagaagctag acaattagtg actcatggtc
    14941 atttcacttt aaatggaaat aaagtagaca taccttcatt aatagttaag gttggagatg
    15001 ttatagaagt taaagagaaa tcaagatctt ctgcaaaatt caaaaattta gtagaagtta
    15061 actcaagaat agctcctaag tggttagaag ctaatgtaga aggaatgaca gcaaaagtag
    15121 ttggtgttcc aacaagagaa gatatagatc ttgagatagc agagcactta atcatagaac
    15181 tttactctaa ataataagca cacttttaaa aattatttta aaattttgtt taccctcagt
    15241 ggattactga attttaagga gggttttgtc catgatagaa atagaaaagc caaaagtaga
    15301 tatagttgag cttagcgaag actatagata tggtaagttt gttatagagc ctctagaaag
    15361 aggatatgga ataactatag gtaacgcatt aagaagaata ttattatcat cattaccagg
    15421 tgtagcagtg aatgctataa aaatagatgg agttcttcat gaattttcaa caatacctgg
    15481 tgttaaagaa gatgttactg agataatatt aactttaaaa gagctttcag caactataga
    15541 tggtgaagga agtagaacac ttaaaataga agctcaagga ccatgctcta tcacaggtgc
    15601 agatataatc tgtcctccag atgttgaaat attaagtaaa gatttagcaa tagctacatt
    15661 agatgataat gctaaactta atatggagat atttgtagat aaaggtagag gttatgtttc
    15721 tgctgaagaa aataaaacag agaatgttcc aataggtgtt ttacctgtgg attcaatata
    15781 tactcctgtt gagaaggtta gttatcatgt ggaaaacaca agagttggtc aaaaaacaga
    15841 ttatgataaa ttagtattag aagtttggac taatggtagt ataaatcctc aagagggaat
    15901 atcattagct gcaaaggtat tagttgagca tttaaactta tttatagact taactgaaca
    15961 tgtaagtagt gttgaaatca tggtagaaaa agaagaagat caaaaagaaa aagttcttga
    16021 aatgactata gaagaattag atttatcagt tagatcatat aactgtttaa agagagcggg
    16081 aattaataca gttgaagaat tagctaataa atctgaggat gatatgatga aggttagaaa
    16141 cctaggtaag aagtcattag aagaggtaat acaaaaacta gaagaacttg gattgggatt
    16201 aaaaccaagt gaagaatagc gagcaaagga gggataagaa tggctaagta ccgtaaatta
    16261 ggacgtgaaa cagctcacag aaaccttatg ttaagaaact tagtaacttg cttactaaga
    16321 agtggaagaa tagaaactac agtgactaga gcgaaagaaa ctcgtagaat ggctgaaaag
    16381 atgataactc ttgctaagag aggagatctt catgctagaa gacaagtttt agcttatgtt
    16441 atggatgaaa cagtagttaa taacttattc acagatttag ctccaaaata tgctgagaga
    16501 aatggtggat acactagaat aataaaaata gggccaagaa aaggcgatgc tgctgaaatg
    16561 gcttttatag aattagtata gtaaggaaag gattaggctt gatggcttaa tctttttttt
    16621 atttttttga ctaaggtatg atataataat gtgtaaaatg atttttataa gttattgctt
    16681 actaaaagga gtatgagtta atggataata tagtaaaggt aaataatatt tcgtttgagt
    16741 atattacaga tgaagcaaaa cttaaagcaa tagataattt aagcctagat gttaaaaaag
    16801 gagaatttgt tgcgataata ggacataatg gttctggtaa gtctacttta tctaaaaact
    16861 taaatgctat tcttatgcct actgaaggaa atattcttat tgatgatatg gatactaaag
    16921 aagaagaaag attatgggat ataagacaaa ctgcaggcat ggtatttcaa aatccagata
    16981 atcaaatagt tgctactata gtagaagaag acgttgcatt tggacctgag aacttaggta
    17041 tagaaccaaa agaaataaga cgaattgtag aggagtcttt aaaaagtgta ggtatgtatg
    17101 atttaagaga tagacagcct catttgttat caggaggtca aaagcaaaga gttgcaatag
    17161 caggaattat tgctatgagg cctaaatgta taatatttga tgaagcaaca gcaatgttag
    17221 atccatctgg aagaaaagaa gttatgaaaa caataaaaag acttaataag gaagaaaata
    17281 taactgttat acatataacg cactttatgg aagaagcagt agaagctgat agagttgtag
    17341 ttatggaaaa aggaaagaaa atcttagagg gaactcctag agaagtattc agtaaaataa
    17401 agatgttaaa ggaaatcggt ttagatgtac cttgtatgac agagttatct agtttattaa
    17461 tagaagaagg tataaatatt agtagtgata tattaactgt ggatgagatg gtgatggaat
    17521 tatgtcaatt atagtaaaga atttaacaca tatatataat gaagggatgc cttttgcaag
    17581 taaggcactt gatgatgttt cttttgagat taaagataga gattttgttg gtcttatagg
    17641 tcatacaggc tcaggaaaat ctacattaat acaacattta aatggattac taaagccttc
    17701 ctcaggagaa atatttataa atgattttaa tataacagat aaaaacttga atttaactga
    17761 gattagaaaa agagtagggg ttgtatttca atatccagaa taccaattgt ttgaagaaac
    17821 gattgataaa gatatagctt ttggaccctc taatttaggc ttagaggagt ctgagataca
    17881 taacagggta aaagcatcaa tggaagctgt aggacttgat tatgagggat ttaaagacaa
    17941 atctcctttt gaattatctg ggggacaaaa gcgtagagta gcaattgctg gagttatagc
    18001 catgaaccca gaagttctca tattggatga accaactgct ggtcttgacc ctggtggaag
    18061 agatgaaatt tttaacttaa taaaagactt acatgaaaaa aagaatatga ctataatact
    18121 atcatcacat agtatggatg atatggcaaa gttagcaaaa actttaattg ttatgaatca
    18181 tggtagcgta gaatttatgg gaactcctag agaagtgttt aaatcaaatg ctagtaaatt
    18241 aaaagatata ggattagata ttccacaagt tttagaactt gcattgaaat tgagagaaaa
    18301 aggctttgac atcagtgagg atatattgac tttagaagaa gcaaaacagg aaatattaaa
    18361 agttgtgaga ggacgaggat tatgttaaaa gatataacta tagggcaata ctacccaaca
    18421 agttctgcta ttcataaatt agatccaagg ataaagcttg tagcgacaat tgtatttatg
    18481 gtatctatat ttgtagtaaa taaattttgg ccttatatag tagttttatt atgtttatta
    18541 gctatgataa aattggctaa tataccagtc aaatatattg ttaagggtgt taaacctctt
    18601 aaatggatta ttttgtttac atttcttata aatatttttt tcttaccagg tgatgaaata
    18661 tggtcatttg gatttttagc tattacaaaa cagggtctaa gacaagcaat ttttatggca
    18721 ataagattaa tatttttagt agttggtaca tcattgctta ctttaacaac ttcaccaata
    18781 gaattaactg atggaataga gagattgttg aatccattta agaaaattgg tttgccagtt
    18841 catgaacttg caatgatgat gacaattgct ttacgtttta ttcctacatt gttagatgag
    18901 acagacaaaa taatgaaggc tcagatgtct agaggtgcag attttgaaag taagaatctt
    18961 ataaatagag caaagaattt agtaccactt ttggtacctt tatttgtaag tgcatttaga
    19021 agagctgatg aattggctat ggccatggag gccagatgtt acagaggtgg acataatagg
    19081 actaagatga gagagtctgt tatatcaaga agggattata tggcatgtgt atttcaggtt
    19141 gtttatctgg gagcaattat agctactaga tttatagcaa tttaattaat aagttataga
    19201 tataatgagg taaagtatga gaaatataaa aattaaaatc caatacaatg gtaagaatta
    19261 ttgtggatgg caaaagcaac ctgattcttt agggatacaa ggtacaattg aaagggctat
    19321 atatgatata acaaaagaag aaaccagtct tatagggtct ggtagaactg attcaggagt
    19381 tcatgctatt ggtcagattg caaattttaa aattaattca ggtataagta tagaaagtat
    19441 accaatggca ctaaatgcca agttgccaaa agacatatca gttatagaag catgtgaagt
    19501 aaatgatgat tttcactcca gatacagtgc aaaaggtaaa acatataaat atttagtata
    19561 taatagcaag tttagaaatc caatacttag tgagatatca tatcaagtaa aatatgaact
    19621 tgattttgat aaaatgtgtt cagaagcaaa aagtttatta ggaacacatg attttaaagg
    19681 ttttatgagt tctggttcat ctgtaaaaga tacagttaga acgatatatg atattgatat
    19741 aagtaaaaaa gatgacttga ttacttttga gattagtggt aatggttttt tatataacat
    19801 ggtgaggatt attgttggaa cacttgttga tatgggtcgt ggcagaatta acgaaccatt
    19861 tttagatata atacaatcaa aaactaggtc aagatgtgga catacagctc cagcacaagg
    19921 attatttctc aaaaaagttc attattaact tgacacacca ggctacatgt attaaaatat
    19981 atgagaatgt gttaataagt ccacttgccc cggacttgac ataaacggta aaaaaatttt
    20041 atgaaaagta gttaaggagg gacagttatg aaaagttata tagctaagcc agctgatgta
    20101 caaagaaaat ggtacttagt tgatgctgaa ggaaaaacat taggtcgttt agcaacagaa
    20161 attgcgacag tattgagagg taaacataaa ccaacattta ctcctcacgt tgatggtgga
    20221 gattttgttg ttgtagtaaa tgcagaaaaa atagttttaa gtggtaagaa attagatcaa
    20281 aaatattaca gataccatac tggttatgta ggtggattaa aagaaatatc ttacagagat
    20341 atgatggata agaaaccaga agaagttata tcacatgctg taagcggtat gttacctaaa
    20401 aataaattaa gaagcagaat gatgactaga ttaagagtat ttgcaggtgc tgagcacact
    20461 catgcagctc aaaatccaga agttttaaac tttaaataag attaataggt gaaaggagga
    20521 gtttataatg gctaacgttc aatattatgg aactggaaga agaaaaagtt ctgttgctag
    20581 agtaagacta gttgcaggtg aaggaaatat attagtaaat ggaagagcat tagaaaatta
    20641 ttttaattat gagacattaa taagagatgt taaacaacca ttagttttaa ctggtaatga
    20701 aaataaatat gatgttatag taaaagttga aggtggagga ttcactggac aagctggagc
    20761 tataagacat ggaatatcta gagctttatt aaaagctgat ttagatttaa gacctgcttt
    20821 aaagaaagaa ggtttcttaa ctagagatgc aagaatgaag gaaagaaaga aatacggatt
    20881 aaaagcagca agaagagctc cacaattctc aaaaagataa ttacttattt ttttggaaaa
    20941 gcaaaatcct ttatttactg ttattcagaa agtattggta ttttaaaaat gttgggttaa
    21001 gttaattatt gatatataat aaaataataa gatatcttat taggatgttt ttcattaaga
    21061 agaacatcct ttttattttg actatttaat aaaaaacata tgctaataag atttaaattt
    21121 tatt
//
LOCUS       DAMJWS010000076        20686 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMJWS010000076 DAMJWS010000000
VERSION     DAMJWS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.077"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..426)
                     /locus_tag="PC399_002834"
     CDS             complement(<1..426)
                     /locus_tag="PC399_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593683.1"
                     /translation="MKIHSIQNLTLDTRNLIKKYSLTLNEDLIWEFKHGKYHTIKYFT
                     HKFAVKESVLALLFNIHKLCYAKIKYFESSFSKYEPYKYSYQNEFVKCEMYDMNFMFH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQDLII"
     gene            complement(827..1288)
                     /locus_tag="PC399_002835"
     CDS             complement(827..1288)
                     /locus_tag="PC399_002835"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF2593684.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
     gene            complement(1393..2586)
                     /locus_tag="PC399_002836"
     CDS             complement(1393..2586)
                     /locus_tag="PC399_002836"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF2593685.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(2609..3274)
                     /locus_tag="PC399_002837"
     CDS             complement(2609..3274)
                     /locus_tag="PC399_002837"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF2593686.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(3308..4420)
                     /gene="ribD"
                     /locus_tag="PC399_002838"
     CDS             complement(3308..4420)
                     /gene="ribD"
                     /locus_tag="PC399_002838"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF2593687.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(4552..4673)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4862..7344
                     /gene="recQ"
                     /locus_tag="PC399_002839"
                     /pseudo
     CDS             4862..7344
                     /gene="recQ"
                     /locus_tag="PC399_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     regulatory      7433..7544
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7686..8987
                     /gene="thiC"
                     /locus_tag="PC399_002840"
     CDS             7686..8987
                     /gene="thiC"
                     /locus_tag="PC399_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2593688.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9041..9235
                     /gene="thiS"
                     /locus_tag="PC399_002841"
     CDS             9041..9235
                     /gene="thiS"
                     /locus_tag="PC399_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF2593689.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            9228..10031
                     /gene="thiF"
                     /locus_tag="PC399_002842"
     CDS             9228..10031
                     /gene="thiF"
                     /locus_tag="PC399_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF2593690.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            10094..10864
                     /locus_tag="PC399_002843"
     CDS             10094..10864
                     /locus_tag="PC399_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF2593691.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            10881..11984
                     /gene="thiH"
                     /locus_tag="PC399_002844"
     CDS             10881..11984
                     /gene="thiH"
                     /locus_tag="PC399_002844"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF2593692.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            11985..12674
                     /locus_tag="PC399_002845"
     CDS             11985..12674
                     /locus_tag="PC399_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2593693.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            12862..14256
                     /gene="yfcC"
                     /locus_tag="PC399_002846"
     CDS             12862..14256
                     /gene="yfcC"
                     /locus_tag="PC399_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF2593694.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFATIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            14773..16140
                     /locus_tag="PC399_002847"
     CDS             14773..16140
                     /locus_tag="PC399_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF2593695.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            16165..16773
                     /locus_tag="PC399_002848"
     CDS             16165..16773
                     /locus_tag="PC399_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF2593696.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            16821..17192
                     /locus_tag="PC399_002849"
     CDS             16821..17192
                     /locus_tag="PC399_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF2593697.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            17371..17709
                     /locus_tag="PC399_002850"
     CDS             17371..17709
                     /locus_tag="PC399_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF2593698.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            18175..18678
                     /locus_tag="PC399_002851"
     CDS             18175..18678
                     /locus_tag="PC399_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF2593699.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            18725..19684
                     /gene="moaA"
                     /locus_tag="PC399_002852"
     CDS             18725..19684
                     /gene="moaA"
                     /locus_tag="PC399_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF2593700.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            19688..20158
                     /gene="moaC"
                     /locus_tag="PC399_002853"
     CDS             19688..20158
                     /gene="moaC"
                     /locus_tag="PC399_002853"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF2593701.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            20194..20625
                     /locus_tag="PC399_002854"
     CDS             20194..20625
                     /locus_tag="PC399_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF2593702.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         8044 a         2256 c         3488 g         6898 t
ORIGIN      
        1 tataattaaa tcctgtaaat tatcttcaaa gctttctagg taatcacaaa ataatctaaa
       61 ttcttctatg gttttaattt cttgtaaatt tctcaaatct atcattatat tactatattt
      121 atgaaacata aaattcatat catacatttc acatttaaca aattcatttt gataactata
      181 tttataaggt tcgtattttg aaaaactcga ctcaaaatat tttattttag cataacataa
      241 tttgtgtatg ttgaacaaaa gtgcaagaac agactcctta actgcaaact tatgtgtaaa
      301 gtattttatc gtgtggtatt taccatgttt aaactcccaa attaaatcct catttaaagt
      361 aagagaatat ttttttataa ggttacgcgt atctagtgtt aaattttgta ttgaatgtat
      421 tttcataatt tattctcctc ataaatattt aataagtatt aaattatagt gttttattat
      481 taattgttct taattatatt gtatggtaaa ttaaaaaagt aataaaatag ataattaata
      541 tactagtaat atatgctata aataaaaaca atagctaaaa tagtaatata tatgttttta
      601 tgatatttta tgtttagggt tatatttgta attattttaa gcttctaaaa gtagatttga
      661 tggataacgt ataattttag aaggatatgc aatttgtttg ataagaacgt acatatgatt
      721 aaattaaaaa tattaagtac caaaaagaag aaagatagtt tctccctttg gtacttaata
      781 ttttgtatcc tttacactaa tttttaatat ttattaatga tattatttaa tctagagact
      841 taaataaatt tgccatttct agcgcattca tagcacaatc ataaccttta tttccagctt
      901 ttgtacctgc tctttcaata gcttgttcaa tattttctgt agttacgatt ccaaatataa
      961 caggtaattc actgtctaaa gatactttgg ctatcccttt agagacttca ctacatacat
     1021 aatcataatg agaagtagcc cctcttataa cacagcctaa acagattata gcatcatact
     1081 taccactttt agccattttt tgagcaacaa gaggaatctc aaaagctcca ggtacccaaa
     1141 ctatttctat attttctgga gaaacgtcat gtcttaaaag acagtcttca gctcctgata
     1201 aaagttttga tgttataaat tcattaaaac gagaatttat tatacctatt tttaaatctt
     1261 ttccaataag ttttccttca tatatcattt ttacttcctc caattttatt tatgttatag
     1321 atttatattt ataaatatct ttaatttaat tataaaaaac tacttagtag aaaaatatca
     1381 tcatataatc atttatataa tatctaaaag atgtcccatt ttatcttttt tagtttttaa
     1441 atatagctta ttaaagtcat taatttcttc attaataggg attctatttt cgatttttat
     1501 tccatatttc tctaattgat ttattttatc tgggttatta cttaaaagat ttatactttt
     1561 tatgtttaaa ttttttaata tttgagcagc catatagaaa tctctcatat cttcttcaaa
     1621 tccaagcatt aaatttgctt caacagtatc gtaaccttct tcttgaagtt tataagcctt
     1681 aattttattt aaaagaccaa tacctctacc ttcttgcttc atataaatta aaactccact
     1741 tccattttcc acaattttct tcatagaaga ttctagctgt aatccacaat cacattttag
     1801 agagtggaac acatctcctg ttaaacattc agaatgaagt ctaactagag tattttctat
     1861 atttatattt ccataagtaa gggcaatgtg ttcttcattt gaataagtat ctctatatcc
     1921 tatgatttca aaatttccat attttgtcgg aaggaaagca ctagatactt tttctatagt
     1981 agtttcattt attttgcggt attctataag gtcttttata gtgatgattt ttaaattatg
     2041 cttttctttg aaaatcatta aatcatcaac tcttgccatt gtaccatcat ctttcattat
     2101 ttcacagata agaccaatct ctttaaaacc tgcaagtcta gcaaggtcta ctgaggcttc
     2161 tgtatgtcca tccctgacaa gaactccacc agtttttgct attaaaggaa agatgtgacc
     2221 aggcgttttg aaatctttac ttgtagaatt ttcatcacag aataatttta cagtttcagc
     2281 tctttcatat gcagatatac cagttagagt atctgctcca tctatagtaa cagtaaaggc
     2341 tgtttgaaat gtatctgtat tttctctaac cataagaggt atattaagac ttttaaattt
     2401 tttttcagtg gcaggtagac aaattagacc tctaccatat gtagccataa aatttattga
     2461 ttcaggagta gccatttcag ctgccatcaa taaatctcct tcattctctc tactttcatc
     2521 atcaactacg attaccattt tcccattttt tatatcttca atagcttctt caatagtatt
     2581 aaacatgtaa ttcactcctt tattattttt aaaaaccatt tttaaataaa aaatctttat
     2641 ctatatttga tttatggtta ctactattag aatttaattc cttgtagtta ttagccataa
     2701 aattattaac atactttcca attacatcgc tttctaaatt aacgaaatct ccaacattct
     2761 ttgtcaagag aatagtgtta attttagtat gaggtataat agaaacctta aatatttcat
     2821 catctaaata tgatatagtt aaacttacac catcaatagc aacagaacct ttgagtatca
     2881 ttgaggataa aagtttttta tcaggtcgta tacttactaa aactgcattt tcatcttttt
     2941 caaatttgca gatagttcct ttaccatcaa catgacctgt tactatatga ccaccaaatc
     3001 ttgaagaaag actcatagct ctttcaagat ttaccttgtc atttgcattt aataaaccta
     3061 aatttgtgga acgaattgtc tccatcataa catcggctgt aaattcattt tttccgaggt
     3121 ttgaaacagt tagacaaact ccatttacgg ctatactatc tccaagattt gtgccatcta
     3181 atattttatt tgatagtatg gttacttttc cactcttacc attagcagta atttttctaa
     3241 gtattccaac ttcttctact atgccagtaa acatcctaac ctcctatatg aatgtattaa
     3301 caaaaattta atttaaaaca tctgattcta taagaatatc gccatctatc aaagttacat
     3361 tattgattgt taactgtgtt gaatctgaca aatgatttat tccttctcca gaaacgaatg
     3421 taggtgcatc tttaccacct ataatcttgg gtgatagaaa taatttgact ttatcaacta
     3481 acttatttct aaagagactg tcattgagag tagcaccacc ttcaactaga acagaatcaa
     3541 tgtttaactc tcctaattta tacattacaa actccaaatc tactaagttg ttttttgaag
     3601 gagaaattat tgtttcaaca ttattttctt ttaacataga taattttata ctttctttgc
     3661 aacatgtgaa aactatagtt ttactggttt tatctttaac caaattacta ttaagtggca
     3721 actttaggtg tgtatctatt actattcttg taggatgaga cactttttct tgattgagcc
     3781 tacaagttag taaaggatta tcgttcaaaa ctgtatttat gccaaccatt atactttggt
     3841 atttatttct gtatttatga gttagatacc ttgatgattc attagatatc cattttgatt
     3901 ctagtgattt tgtggcaatt ttaccatcta gacttactgc tgattttaca atacaaagag
     3961 gtcttttgtt ttttatatga taaaaaaatg cctcatttaa atctcttgct tcatcttcta
     4021 atattcccac agtaacatca atattgttat cttttaattt ttttactcca ttaccacaaa
     4081 caagtggatt tgggtctaat gtagatatta caactctctt aatcttattt tgtataattt
     4141 tatctacaca aggaggcgtt ttcccataat gagaacatgg ttctaagttt acataaagag
     4201 tagagtcttt tagactttgt ttagcactgt ttatagcatt tacttcagca tgattagagc
     4261 caaatttctc atgatatcct ttgcctatta tattactatc tttaacaatt acacatccaa
     4321 ctaatggatt tggatttaca aagccttctc catttttagc caattcaata gcttttttca
     4381 tataataaat atctttttct ttttgattca caaaattcac aatatgtaca cctcctgttt
     4441 aattggataa tttttaaaca aaaaaccctg agaattaact cagggtttag aataatctac
     4501 aataaatgtt taataaatgt agaacaaaat tgctctacaa aaacactcat taaatatctt
     4561 cttccatcca gactttactg tcggctttgg aatttaacca aatcaaccaa gttttacatt
     4621 ggctcgcggg ctataccgcc gattgggaat ttcaccctac cccgaagatt aagtattaaa
     4681 tttatattta gaatgtatca taaattattt tactttgcaa actatttttt taaaaaatat
     4741 atgttaatat ataatataga atgtctataa actttgaaat accacgattg tgaatataaa
     4801 aataaaaatt tgaacataat caggaaagaa gtctaattaa tttaaattag gagagtaata
     4861 tatgaataaa aaacttttag aaatactatc aaaatactat ggatatacaa gttttagaaa
     4921 aggtcaggaa tctataataa attctatctt aagcgaaaaa gatgtgctag ctattatgcc
     4981 aacaggaggg ggaaaatcaa tatgttatca aattccagct ttgattttag atggaatgac
     5041 aatagtaatt tctccactta tatcacttat gaaagaccaa gtagatgcct taaagactat
     5101 gggaattagt gctgctttta taaatagttc attaagtagc aaagaattta atgaaatatt
     5161 aaataatatt agaaaaaata attataaaat actctatatt gctccagaga gattagatgc
     5221 acaagagttt attgagctaa taaataataa caatgtaagt caagttgcga tagatgaagc
     5281 acattgtgta tctcaatggg ggcatgattt taggttgagt tatagaagga tttctgattt
     5341 tataaaaaat ttgcctaaaa gacctatagt tacagcattt actgcaacag cttcagaaga
     5401 ggtaagaact gatataataa atttactgtg tttagaaaat ccagattatt atataactgg
     5461 atttgataga gagaatttat caataaatat agtaaaatca tctagtaaaa ataagtatat
     5521 tttagattat attcaaaatc ataaaaatga gtctggaatt atatatgttg caactagaaa
     5581 ggaagttgaa aatatatata atggtttatc taagagaaat atatctgtat caaaatatca
     5641 tgcaggttta tctaaaaatg aaagaagtaa aaatcaagaa gattttataa atgataatgt
     5701 ggatataatg gtagcaacaa atgcatttgg aatgggtatt gataaaccta atataagatg
     5761 ggttatacat tacaatatgc ctcaaagtat agaaaactac tatcaagaaa taggtagagc
     5821 tggtagggat ggagaaaaaa gtgagtgtgt acttttattt tctccaggag atgtacatat
     5881 acaaaaatat ttaattgata ttggagttga aaatcctgaa agaaaattat ttcagcataa
     5941 gaagcttcaa tacatgattg acttagttta cagtaattca tgctatagaa agacaatatt
     6001 gagttatttt ggagaaaatt atatagacaa ttgcaataat tgtagcaatt gtttagtaga
     6061 gggtgaaata gtagataaaa ctatagatgc acaaaaagtc atttcatgtg tagctagaat
     6121 gaaaagaagt tatggtgtta ctactattgt agatgtgctt agaggttcta agaataaaaa
     6181 gattttacag cttggattaa atactttgtc tacatataac attatgagag attattctag
     6241 tgaagactta aagaattttg taaatacatt ggtttctcat ggttttttag atttagttga
     6301 gactctaggc aatggaaata gaggtagttt tcctactata agattaaatg agatgtctat
     6361 gaaagtttta aaaggtgaag tgaaagtaga gtttaaagaa attgttatga caaaatcatt
     6421 ggaagtagag gatgagttat ataatgcttt aagagaactt agacattcaa ttgcaagtga
     6481 agaaaggata gctccatata tggtatttgg agatggtaca cttagagcca tgtctagtag
     6541 ttatccaatt aataaagaag aaatgctaga tatctctgga gtaggagaaa ttaaatacca
     6601 aaaatatggt agagattttg aagcagtaat taaagaatat gtcgaaaaaa taatataaat
     6661 aaaaaaatag atgaaaaaaa tgtcgattca aagaacgtta atagtgattt cttagaggtt
     6721 aatacagata aagttttata tgaaaaactg ttagtcatta gagaagaata tgcaacaaag
     6781 gaaaaattac ctgcatatat gattttagcc aataaggctt tgaaagaaat aagtggaaga
     6841 tatccacttg atgaagaaca attaaaagat atttctggca taggtggagt aaaatttgaa
     6901 aaatatggag ctagtatttt ggcagaggtt aataaatatg taaaagaaaa taatattaat
     6961 gttatttggg agaaaaaggg caaaaggaag ttaatattag atggagaaag tagaaaaaat
     7021 aatgaaatag cattagattt gctaaatcaa ggaaaagata tacaaagtat cagtcaagaa
     7081 atagaagttt cactgtcaac cattattgga tatgtgcatg actatgtaaa ggatggacat
     7141 agtatatcat ttgaattagg actagaaaat ttttataatg aagataaaaa gaagatgatt
     7201 ttagaagttt gcgaaaaagt tggctatgat aatttaaata acataaaaag aaaacttcca
     7261 gattcaataa agtatgaaaa tataagagct gtgatacttg actcttatct tgaaaactta
     7321 aaaaataata ttatagcaga gtaattttta acttaataaa aaataaaatt tgtattattg
     7381 tgttgaacta ataattttgc tgtgttatac tgaatttgta aaaataatga aaaattagag
     7441 ctaggggcgc tagaattggc tagctgagag ataaagccgt aacttacaaa ccctaataac
     7501 ctgatcaaga taatgcttgc gaagggaagc tttttaatat aattttatta tataaatagg
     7561 caaattattc aatgttagga gaaatttgtc taagtgtata tacgattata agagtgcttt
     7621 ccaaacgagg aaaagcactt tttttatttt caaaaactat aactttaatc ttggaggatt
     7681 gtaatatgaa ctatacaaca caaatggatg ctgctagaaa aggcataata actaaagaaa
     7741 tggaaatcgt ttctcaaaaa gaacaggtag atgtaaatga attaagagaa ttaatagcaa
     7801 atggacaagt tgttatacca gctaataaaa atcataaatc tttaagtgca gaaggtgtag
     7861 gtaaaaattt aagaacaaaa ataaatgtaa atctaggaat ttcaagagat tgtaaggata
     7921 tagaaaaaga acttgaaaaa gttagagtag caatagacat gaaagctgaa gctataatgg
     7981 atttaagtaa ttatggcaaa actagagaat ttagagaaaa ggttgttgaa atgtcacctg
     8041 caatgatagg ttcagtacct atgtatgatg cagtaggata tttggaaaaa gaattaaaag
     8101 atataacaga agaagagttt ttaaatgtaa taagacaaca tgctattgat ggagtagact
     8161 ttattactat acatgcaggt cttacaagaa gtgtttgcca aaaaataaaa aatcatgaga
     8221 gattgactca tatagtatct cgtggaggtt ctttattgtt tgcatggatg gaattaaata
     8281 ataaggaaaa tcctatttat actaattttg ataaaatatt ggatatatgt gaagaatatg
     8341 atgttacatt gagtttagga gatgcttgta gaccaggttg tataaaagat tctactgatg
     8401 gtgttcaaat tcaagaactt gtagtattag gtgaacttac taaaagagca tgggaaagaa
     8461 atgtacaagt aatgatagaa ggtcctggac acatggctat tgatgaaata gaagcaaatg
     8521 tagtattaga aaagagatta tgccatggag caccttttta tgttcttgga ccattggtta
     8581 cagatatagc accaggttat gaccatataa caagtgcaat aggaggagct ttagcatgtg
     8641 ctaaaggagt agatttttta tgttatgtaa ctccagcaga acatttaaga ttgcctaatt
     8701 tagatgatat gaaagagggt ataatagctg caaagatagc tgctcatgca ggagatatag
     8761 ctaaaaatgt taaaggtgct cgtgaatggg ataataagat gagtaaagct agagctgatt
     8821 tagactggtg tgagatgttt agacttgcaa tagaccctga aaaagctaag agatacagag
     8881 atgaatcaac tcctactcat gaggatagtt gtactatgtg tggaaaaatg tgctcaatga
     8941 gaacagttaa gaaaatatta aacaatgagg aacttaatct aatataattt ttggctagta
     9001 tgactggaat aaaaataagc tgaaaagagg aggtgattta gtgaaggtta atgggaagga
     9061 aattgaattt gaaaaagatt tgactgttat agatttatta aataaatata atttaaaaag
     9121 tgatagagtt gtagttgaag tgaatttaga gataattgaa gaatctaact acaatacata
     9181 tgtattaaaa gacgaggaca ttgtagagct tattagcttt attggaggtg gttaaaataa
     9241 aaatatttgt aaatgaatta ttaacagatg ttgaggaaaa tacaactgct tataaagtta
     9301 gagatagcta taacaataaa tgtgatgtat tggtgttaaa tggatatcca attaaaacag
     9361 atatgaccct ttgtgaaaat gataaattaa cacttataca aaaaggagtt aagccatcac
     9421 ttgatgaatt ggaaagcttg ttgattgcta gacatactcc aaatatacat aataaattaa
     9481 aaaaagggaa agttgctata ttgggattgg ggggacttgg ttccaatata gctatctctt
     9541 tagctagaat aggagtggga gaacttttac ttatagatta tgatgttgta gaaccatcta
     9601 atttaaatag acagcagtat tttatagatg atataggaaa actaaaaact gatgccatga
     9661 tagaaaatat aaaaaaaata aatccattta taaaattaaa taaacgagat atttttttaa
     9721 acaagaacaa tatggatact attaaggatt cagatttaat aattgaggcc tttgatgatg
     9781 cttcatgtaa agcacaggta tgtaattatg tattaataaa tttaaaagat aaatatttaa
     9841 tagcatcatc tggtatggca ggttactatg attcaaatat aataactact aagaagataa
     9901 aagacaggtt ttatatatgt ggggattttg tgaatgaagc taaatttggt gaagggctta
     9961 tggctccaag ggttgccatc tgcgcaaatc atatggcaaa tttggcaacg caaatactta
    10021 tagatatgta aatattaaaa ttagatataa aaaagattga atattaaaaa aatttaacgt
    10081 ggagggaaat aatatggata aattggtact tggtggacat gaatttaata gcagattatt
    10141 ggttggaaca ggtaaatatg gttcaaataa tatattgcct gaggtaataa aagaaagtgg
    10201 aagtgaaata ataacaatgg cacttagaag agtggattta gacaataaac aagaaaacat
    10261 attaacgtat attccaaaag agatgacaat acttccaaat acatcaggag ctactaatgc
    10321 agaagaagct gtaagaatag ctagaatatc tagaaaaatg ggatgtggag attttataaa
    10381 aatagaagta atatcagata ctagatattt acttcctgat aatgaagaaa ctataaaagc
    10441 tacaaagata cttgctgatg aggggtttat agttcttcca tatatgacac cagaccttta
    10501 tgctggaaga agacttatag aagctaatgc agcggcagtt atgccacttg gagctccaat
    10561 tggttcaaat agaggtcttc aaatgaaaga aatgataaga ataatgatag atgagttgga
    10621 tataccaata atagtagatg caggtattgg aaaaccatct caagctatgg aggctatgga
    10681 aatgggagct gatgcagtac ttgtcaacac tgctatagct tcagcaggag accctgtaca
    10741 aatggctaga gcatttaaat tagctgtaga aggtggaaga gaagcatata ttgcaaaaac
    10801 aggaaatgtg tcagaatttg caaatgcatc ttctccttta acaggttttt taggcaattt
    10861 atagaaagtg gtgattggtg atgagttttt atgatgtaat agaaaaatat agggattttg
    10921 attttgatgg atatttaaat aatgttactg ataatgatgt tttaagaagt ttatcaaaag
    10981 acaagcttga agattttgat atattaaatt tgctttctaa aactgcagta aaacatttag
    11041 aagatatggc acaaaaagca cataaattaa gtgttcaata ctttggtaaa acagtatgtt
    11101 tatatacacc aatgtatata gcaaattatt gtgttaatca atgtgtttat tgtagctata
    11161 atataaagag tggaataaaa agaaaaaaat taactatgga tgaaattaga gaagagggag
    11221 aagcaatttc aaaagaaggt ttcaaacatc tacttgtttt gactggagaa agtagttttc
    11281 actcaagtgt agaatatata ggtgaggcta tagaaatatt aagagagaaa tttccctcta
    11341 taggtataga agtttatcca atggaagtag aagaatataa gtacattgta gataaaggtg
    11401 ttgaaggatt aacagtatat caagaaactt atgacgagga aatatataag agagtacata
    11461 taaaaggccc aaaatcaaat tataaattta gactagatgc tcctgaaaga ggggcaaaag
    11521 caggtatgag gactttatca ataggtgcac ttttagggct aaatgatttt agaaaagaaa
    11581 ctttttttac aatattgcat gggaaatact taaaaacgaa atatcctcat attgagcttt
    11641 catattcaac accaagaatg agacctttta aagggtgttt tgaggaattg gtagatataa
    11701 gtgatactga tttagttcaa gctatggtat gtatgagatt atttgaccct catgcagcta
    11761 taaatatatc cactagagaa aatttagaaa tgagaagtca tataatacct ttaggagtaa
    11821 ctaaattgag tgcaggtgta tctactgatg taggaggtca ttctcaagat gaacatgata
    11881 ctgctcaatt taagataaat gatgaaagta ctgttaaaga tgtggagaaa atgctaaact
    11941 caattggtta tcagcatgtg tttaaagatt gggaaagatt ttaaatgtat ctgataacaa
    12001 acagaaaatt atgtagtgag gaacgatatt tagaagttat taaagagtct atattgagtg
    12061 gtgtagaaaa tataataata agagaaaagg atttagaata tcaagagcta agaaaattat
    12121 atatgaaaat aaaaactaaa atcaattgta tagattttca agagcaaata tcagatgaaa
    12181 gtttgaaaac taatataaat caaaaagagt gtagaaataa attcaaggtt aattttataa
    12241 taaatagtaa cattgaattt tttgaaaaag tggattgtca aggtatccat cttcctttta
    12301 aattattctt gaatttaatt gaaaataagt ataattttaa tgaaaataaa atactaggat
    12361 tatctttaca taaagtagag gaagtagatt atttagaaaa attaataaga aatcaaaata
    12421 taaaaataga ttacataaca ttatcacata tatatgaaac taaatgtaaa gaaggattaa
    12481 atccaaaagg aattgagctt ttaaaagaag ctaaaaagat tactgatatt aaaataattg
    12541 cacttggggg aattttacca tctaatgtca aggaaacatt aaaatactgt gatgattttg
    12601 ctataatgtc aactataatg aggtctaaag atattaaaaa aaccatatca aattataatg
    12661 aaaagttgaa ttagtaaata tagtatattg aatttttaac ttaaataaaa ataaaaaagt
    12721 tatcttttaa attttaaaag gtagcttttt tattttatat ctttgctaat aaaaaattgt
    12781 aaaaagggaa aactttagtc agagagatta aacaatatcc tagaaagatt taaaatttag
    12841 taaagtatgg aggacattat aatgactgac agtaaaaaga aacgatttat tataccacac
    12901 acttttacaa taatattttt actaatagtc ttgatggcaa tattgacttg gatagtacca
    12961 agtggtgaat ttagtagaga agatgtagaa ggtagaatgg tagttgtacc agggacttat
    13021 caaagtgtag aaagtaatcc tcaaggattt gcagatgttt ttaaagcacc aatagaaggc
    13081 tttatcgatt cagctgaagt tgtaggtttt gttttaatag ttggaggtgc ttttggtatt
    13141 gtaaatagga ctggagctat agaagcagga atagcttcgg ttatctctaa gtttaaggga
    13201 aaagaacttc ttataattcc aatatcaatg attttatttg gacttggagg tacaacattt
    13261 gggatggcag aagagacatt accattttat atgatatttg ttccactcat gacatccttg
    13321 ggatatgatt ctctaacagc aatagcaatt gtatttatag gagcagcagc aggagccgcc
    13381 gcttcaacaa taaatccatt ttcagttggt attgcacagg cactagctca acttgctcct
    13441 ggttcaggag taacttacag agtagttatt tttatagcat atatactaat aagtatagca
    13501 tttgttatgt actatgctaa gaaggtaaag gaaaatcctg aaagttcaat agtatatgaa
    13561 atagataaaa ataataaatc aatacttaat caaacttcaa cagaaataaa agaatttact
    13621 tttaaagaag tatctgttct tgctatattt ggaataggga tggcaataat gatttggggt
    13681 gttttaagtc aaggatggta cattccagaa atatcaatgt gttttgctac aataggtgtg
    13741 ctttctggat tggttggtag attatctcaa attgaaatct cagaaagttt tatagatgga
    13801 gcaaaagatt tagcatctgc agctcttgct atagctttgg ctcgtggaat cgtaataatt
    13861 gctcaaaatg gatttataat agatactata ttaaattccg cagcagcctt tctaggtggg
    13921 cttccaaagg ttatatttgt atatttatca tttttcttgg aagctgcatt ggcattttta
    13981 attccatcat cttcaggtct tgcttcactt acagtacctg tacttgctcc tcttggtgat
    14041 ttggttaata tatcggctca agtgatagta accgcttatc aatttggtgt gggtcttaca
    14101 aactttataa ctccaacttc aggagtactg atgggagcac ttgcagtagc ccatattcca
    14161 ttttcaaaat gggtaagatt tataatgcct atcttgttga ttttgacagc tgtatctcta
    14221 gtctttttaa caataggtat ttatataggg ttttaattaa gtgtagtttt atttaagata
    14281 tttagtaatg gtataaaatg ataatatgtt ttcaataaat tgccactaat ttaattagtg
    14341 gtaatttgtt ttttgtattt aaaaataaat atatttactt gtattttgaa ttaatatcta
    14401 cttagacatt gttttaattg ttatattttc atttaattaa gcattgattt atcatttttt
    14461 atatttcata taacaatgca aaaaataatt tttatatagt aaatgacaaa agtttacaat
    14521 aaatgtatat tttcttactc attccttaaa attactcatt tagtttaata actctcattt
    14581 catatgtaaa gttggtaaaa aaattaaaat taaaagtaaa aaactaaata aattaatgtt
    14641 aatttgaaat atatatgaag gtttttggag aatacttact tatgatagga aaaaatgtta
    14701 aaataatagt gtaatcaaat ataaaggact gaaataatta acagttatta aatattagga
    14761 gtgtaaaaat ttatgggatt aaaagaattt gattttattg aagagtctat tgatatgttg
    14821 aggacaatgt caccaacatt agaaactata tcagatgaaa tagaagagta ttttgaaaat
    14881 atcttggatg aaaagaatca agaatatata aatgtaacct ctagaataaa atcagaatca
    14941 agtttaagag aaaagataat tagaaataga tatttaaaaa aatatggaga agcaagtaat
    15001 cttattcata atgtttctga cttaataggc ctaagaattg agtgtagatt tattgaggat
    15061 gaaaacaaaa tatatagact tttgagaaga tattttaata aaacagatga caaaataaac
    15121 tattataata aagagaataa gaatattaaa ttaaaactat ctgagagaca acccaataag
    15181 caaaaaaatg gatttgaaat atataaaata gatggagttt ttgcgtattt agatagagaa
    15241 gtaaaatttg aacttcaaat taaatctctt gtaaatgtgt tttggagtga aatagaacat
    15301 aaaataattt ataaaaacaa tacatattta cttgctgata aatttataaa ggatatgatg
    15361 gactctataa agaataatct tactatgata gataatcagc ttttaagtat ttataaaaac
    15421 tttcattcag ggaagtcttt taatatgaaa gtaagtaaaa aagaaattga aaaattattt
    15481 gcgaaactag tctatgacgc tttttctgaa aagatgaata aaagtatagg ttttgttgta
    15541 gattttaaaa aaccatgtga gactatattg aactattctt ttaataaaca agatataagt
    15601 gatgaagttt taggtaattt tatgcttgat gagtttgcaa gattaaatga aattgtaaac
    15661 aaggatatag attttaatga gcaaatagaa tttgaaagag aaccaacatt tgatgataag
    15721 ttttgtaaag acctaggaaa tcattttaga agtagattaa acacagaatt tccatggaac
    15781 ctatttttta gaatattatt tgaaatagag ccatataaca ataccaaaga ctttgaaaac
    15841 tttgtagagt ttataaaaga aaatgtgcta ctagaagaaa atagggagca attattatgc
    15901 caatttgaag aagatagcaa acttataatt gaagatatat atgattgtat atttaatagt
    15961 ataagtgaga ttgatagtgt tgaaatttta tataactata atcttgaaaa aataaacttt
    16021 acatcaagtg aaatcataaa ttgtatatgt agagaatatg agtgttatgg tgaatactta
    16081 gaagaaaaag aaaagcttat gaatacattt aaagaaaaga ttattcaaat ctttgagtaa
    16141 taggtcaatg ctgaagtggc gctaatgcgc catttttcaa atatatatga attaagtaaa
    16201 attattttgg aggaggcgat ttgtatagag aaaacaggtg taattttggc agggggaaga
    16261 aattctagaa tgggtagaga taaagccttt ttagagttac atgataagct ttttattgaa
    16321 attgcaatag aagcatttaa aaactttgat gagttgataa taatatctaa taatgaagaa
    16381 ttatattcta aatacgatat taaagtttat aatgatatag taaaagatgt tggtccaatt
    16441 ggaggaatat atactgctct tgaatatgca aagtatgata tagttacaat tgcatgtgat
    16501 atgccatatc taaataatca aatagtcgaa agaatagcta ataaaatgaa tgataaaagt
    16561 gtgatttcag tgacaaatgg aaagttacaa ccattgtgtt ctggatataa aaagagtata
    16621 ataaataaag tatctttatg tataagggaa aatgatttaa agctcagaag ctttatagat
    16681 aaaatagata aaagttatat atactttgat gaagaagatt tatttttaaa tgtaaacact
    16741 gttgatgagt atgaaaaatt aactaaagtt taattagcta tggttgtaaa atagagtagg
    16801 atttatatgg aggagagttt atgaatttac ttaaatgcga ttcttgggca gtaatattaa
    16861 ataatagcga taaagaaagt aaagcatata aaatattaga tgaacttaaa aggaatatgt
    16921 acaaagttgt tgctatagat gaagagaaaa aacctattga aggaatagat gtgtatgaat
    16981 gtttaaaaga tgttccacac aatattgatg ttgtagctat tattgataaa caatctaaga
    17041 tggatgtgat tttagaagaa gtagagcttt tggatataca aaatatgtgg tttgaaaaag
    17101 gaagctttag tgaaatgatg attaaaaaga ctaaagattt aaaattaaat atagaatata
    17161 atttaagttt atatgatgag ttgactagat aaaattatat atcttaataa taaaaatata
    17221 attagttaat aaaacatatt attttatagt atagattggt taataaaagt acagattggt
    17281 taatgaaaca ttaaattaca atatttgtta aaaaaattgt gagttacatg atataatgta
    17341 ttaaatagga tactaaggag ttgatatatt atgttaatag taactacaga aaaagtagaa
    17401 ggtaaaaaga tatcaaaggt tttaggatta gtgagaggaa gtacaataag agcaaaacat
    17461 gttggaaaag atataggagc aagttttaag aatcttgttg gaggagaact tactggatat
    17521 aatgaaatgc tcactgaagc aagacaaatt gccataggta gaatggttga agatgcagaa
    17581 gctaaaggtg caaacgcagt aatagcattt agactgtctt cagcttcagt tatgcaaggg
    17641 gcagcagaaa tgcttgctta tggaacagca gttgttttag aagatgataa tagtattctt
    17701 gaaaaataaa atataaatag ttaaaaagag gcatctttta aaaattttat ggatgccttt
    17761 ttttatttga aaaaactaat ttgagaagaa attttaacta ttatgacaat aaaattaggg
    17821 tgatattata taaataagta aaattatatt atataatgta tggtatagac tttaaaaatt
    17881 ttatttgtag agaaaaatat ggacaatgtt ataataatta tagaaattaa aagtacttac
    17941 attcattatg tatgtgacat gtataattag ttttatattt aatacagttt tgaagtaata
    18001 tcttcatttt gagaacgttg gaataataaa aaatgtatta ttataggtga tataaataaa
    18061 aaataaatat aatatgttat attgctgata gaaaagagaa taaaagaaaa taggttttat
    18121 taaaagagat ttttatttat aggacttaat caatgtatag aggggtgaga acatatgttt
    18181 aatgttgcaa ttataacact tagtgataaa ggatatgaag gaaaaagaga agatataaca
    18241 ggaaaaaagt taactgaatt tgttgaaaat acaggagctt ataaagttac tgaatatgta
    18301 cttataaaag atgataagga aatgctaaag gaaaatatta taagactttg taatagtaat
    18361 aaaatagatt taattcttac aaatggaggt actggttttt caaaaagaga tataacccca
    18421 gaagcaacta aggaagtgtt ggaaaaagaa ataccaggtc tgagcgaata tatgagaatg
    18481 aaatccacag aaattactaa aaaggccata ctaagtaggg gtgtaagtgg aataagaaat
    18541 aattctataa taataaattt acctggaagt cctaaaggtg cagttgaaaa tctaagtttt
    18601 ataattgatg ttttagacca tggcatagaa gttttaagag gagaagctac agaatgtgcc
    18661 actaaaaaaa gtgaatagta tataatctag atagataaaa aatagacaaa gaggtagata
    18721 aagtatgatt gataagtatg gaagaaaaat agactacctt cgtatttcac ttacagataa
    18781 gtgtaattta aggtgtgttt attgtatgcc accagatgta aaatttgata aaaattatat
    18841 aaatgaaaat ctaagctttg aagactacaa gtttattata aaggctatgg ctgaacaagg
    18901 tataacaaaa attagattta ctggaggaga acctctttta tatcctaaat taaatgagct
    18961 tataaaattt acaaaagaag aatgtggaat aaataacata ggaatgacca ctaatgctat
    19021 tgggctttct gatagaatct ttgagcttga aaaatgtggc ttaaataaag ttaatataag
    19081 tttagattct ttgaaggaat ataagtataa atccgtaaca agaggaggaa acttaaaaga
    19141 tgtcttaaaa tcgattgaat cttgtttaaa cttaggtata aaagtaaagt taaattgcgt
    19201 tgctataagt gggtttaatg atgatgagct taaagatttt atgtttttaa cagttaaatc
    19261 accaattgat gtaagattta tagagcttat gccattaggt gaggctaata gagtttataa
    19321 gaagggttat tttaatgtaa aagaaatgat tgaaaaaata tatggtttat ataaagtcaa
    19381 aaatgatgaa aaaagtacag cagaatatta tatgtttaaa ggagctaaag gaagaatagg
    19441 aattataact ccattaagct gttcattttg taacacttgt aatcgtatta gacttacttc
    19501 tagtggtgca ataaaattat gtctacattc aaaagaagaa atagatataa aaccattttt
    19561 acataaacca ttgttattta gagaatctat gaaagatata ataaaacaaa aaccagaaaa
    19621 acatcattta atagaaaata aatgtagtga tacaaatagg cgtatgtatg aaattggagg
    19681 ataagtaatg ttaacacata taaatgaaaa agggtatgcc aagatggtgg acataagtga
    19741 aaaagatgat actaaaagaa cggccgtagc tacagctaca attactctta gtaaggaagc
    19801 attagaaaaa gtaaaaaatg gtcagataaa aaaaggagac gtattgtctg tagctcaagt
    19861 tgcaggtata atgggagcta aaaatacatc ttcaaacata ccaatgtgcc atcagattaa
    19921 attagttgga tgtgatttag aatttgatgt agatgattct atgtctgcta ttaagatata
    19981 cgcaacctgt aaatctcttg ggaaaactgg agtagaaatg gaagctttaa atgcatgttc
    20041 tttagcagca cttacaattt atgatatgtg taaagcagtt gataaaaaaa tggttataag
    20101 taatatccat cttgtagaga agactggtgg aaaatcagga gattttaagt tcgagtaaat
    20161 taataatata ttgggttaaa atttggagga aatatggcga aggtagtgtc aataaatata
    20221 agtgagaaaa aaggtgtaat taaagttcct gtacaatgtg cagagctaaa aataaatcat
    20281 gggatagttg gagatgcaca tgcaggaaac tggcatcgtc agataagctt acttggaaaa
    20341 gaaagcatag ataaaatgaa aatgaaaggc tttgaccaat taaaatatgg agattttgct
    20401 gaaaatgtaa ctacagaagg gatagaagtt ttttccctgc ctgttggtac aaagttaaga
    20461 atagggcaat gtgaagttga agtaactcaa atagggaaaa aatgtcacaa tggatgcgaa
    20521 attaagaaat tgacaggtga ctgtgtgatg ccacgagaag gaatttttgt aaaggttcta
    20581 aaagaaggaa ttataaatgt gaatgattca atagatgtaa tctaatgaag tcactattaa
    20641 aatataatta taagtaaata ctattaagat ttaaatatat atttta
//
LOCUS       DAMJWS010000077        20285 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMJWS010000077 DAMJWS010000000
VERSION     DAMJWS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.078"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(484..2088)
                     /locus_tag="PC399_002855"
     CDS             complement(484..2088)
                     /locus_tag="PC399_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2593703.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2474..3400
                     /locus_tag="PC399_002856"
     CDS             2474..3400
                     /locus_tag="PC399_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2593704.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNTYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3520..4518)
                     /locus_tag="PC399_002857"
     CDS             complement(3520..4518)
                     /locus_tag="PC399_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF2593705.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4622..5059
                     /locus_tag="PC399_002858"
     CDS             4622..5059
                     /locus_tag="PC399_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2593706.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            5145..5732
                     /locus_tag="PC399_002859"
     CDS             5145..5732
                     /locus_tag="PC399_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2593707.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(6032..6652)
                     /locus_tag="PC399_002860"
     CDS             complement(6032..6652)
                     /locus_tag="PC399_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HDF2593708.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(6660..7427)
                     /locus_tag="PC399_002861"
     CDS             complement(6660..7427)
                     /locus_tag="PC399_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDF2593709.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7468..8289)
                     /locus_tag="PC399_002862"
     CDS             complement(7468..8289)
                     /locus_tag="PC399_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593710.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8648..10558)
                     /gene="phnW"
                     /locus_tag="PC399_002863"
     CDS             complement(8648..10558)
                     /gene="phnW"
                     /locus_tag="PC399_002863"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF2593711.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10837..11622)
                     /locus_tag="PC399_002864"
     CDS             complement(10837..11622)
                     /locus_tag="PC399_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2593712.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDAMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKAYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11839..12069)
                     /gene="dltC"
                     /locus_tag="PC399_002865"
     CDS             complement(11839..12069)
                     /gene="dltC"
                     /locus_tag="PC399_002865"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF2593713.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(12088..13236)
                     /gene="dltB"
                     /locus_tag="PC399_002866"
     CDS             complement(12088..13236)
                     /gene="dltB"
                     /locus_tag="PC399_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF2593714.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(13236..14750)
                     /locus_tag="PC399_002867"
     CDS             complement(13236..14750)
                     /locus_tag="PC399_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF2593715.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14871..16061)
                     /gene="dltD"
                     /locus_tag="PC399_002868"
     CDS             complement(14871..16061)
                     /gene="dltD"
                     /locus_tag="PC399_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF2593716.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(16349..17368)
                     /locus_tag="PC399_002869"
     CDS             complement(16349..17368)
                     /locus_tag="PC399_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2593717.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17544..18083)
                     /locus_tag="PC399_002870"
     CDS             complement(17544..18083)
                     /locus_tag="PC399_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2593718.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(18184..18864)
                     /locus_tag="PC399_002871"
     CDS             complement(18184..18864)
                     /locus_tag="PC399_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2593719.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18893..20182)
                     /locus_tag="PC399_002872"
     CDS             complement(18893..20182)
                     /locus_tag="PC399_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2593720.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         6683 a         3500 c         2089 g         8013 t
ORIGIN      
        1 ctattatttg gatagaatta ttttttaaat tagaattatt ttgatgaatg attcaagtaa
       61 taggtatgaa attgaatctt ttagaactgc ttacaaaaaa tactcaaaga aaaatagtaa
      121 ttttctcaga ttcacaatat attttatatg aaatgttata agttaatcta tttaataaat
      181 ttaaatttgc tattatagat ttagttccaa agtcttactg caaagtaata tacatagttt
      241 tttaataaac taataagtgc aattaataac tacttaattg tttaatatag tacatagata
      301 actttaataa atctacagtt atttaaaaag gtagaatcaa taaaattata cttataaatc
      361 ttcaatacaa taattaaaat ttaataaaaa tataattgtt gatttaactg taatcttatt
      421 taattctacc taacttatta tataaaacta ttttttatag attataaaat atctaacttt
      481 catctacttc aaagaataca atacttctcc ttctcttata gtctgcaata ctttaatatc
      541 ttttattttc atagggtcga ttgtaagtgg gttttcatct aatattatca aattagctaa
      601 tttcccttct ttaattgaac cttttttatc ttcttcaaaa tattgatatg ctgcatttat
      661 agtaacaccc tttaaagcat ctaatgggct tatcttttca ttttctccta tttgtatacc
      721 tttttttgta attctattga cagcacacca tactgtttcc agcatattag gtgctattac
      781 tggtgtatcc tgatgaagag tgtaaattaa tcccttttct atagttgttt taagtgggct
      841 aatcttaaac gctctatctt ctccaaggtt tttgatatga atatcacccc aataatatgt
      901 gtgagctaca aaatatgaag gtatcatatt tattactttc atatcatcaa tctggtctgc
      961 tcttactgtt tgagcatgta tcataactgg tcttatatta ttttcactac tttgctcttt
     1021 taagtttaaa actttctcaa acgcatctat caactggtct gctgctgcat ctccattaca
     1081 atgagttaag agttgcatct tttcttttag tgaaatatct ataaactttt ctacttcttc
     1141 atctttgtat ataggatatc cacaataacc atcatctgaa ttttcatatg gtctactcaa
     1201 ccatgctgtc ttaccttgtg gagaaccatc taagaaaatc ttataacctc ctattttaaa
     1261 tctatttata tattttttta taaactccct attattatct gctacagact tacttttttt
     1321 caaatttaca tatcctacaa catccatttt taatttattt tgatttgcca ttgcttttaa
     1381 aatgttaaat tgattttctt ccattaaacc ttcttgagct gttgtaatac catagcttag
     1441 atatatgttc tgtgcttttt caattgaatt aaaaatctca tttgatgatg gttgttttat
     1501 tttagatgct acattaaaaa atgcattttc ttctaaatat ccatttggtt cttcactgcc
     1561 ttctactcta cctatatgtc caccttctgg gtcacgtgtt tcattggtaa caccaagctg
     1621 ttttaacccc aatgtatttg caactcccat atgaccagaa gcatgtgaaa ttagtattgg
     1681 attttctgaa gatgcactat cgagtacact cttatctgga tgcttatttt cttctaaaaa
     1741 attattatca tatgaaaatc caattatcca gtctcctttt tttatattat tcgactcttt
     1801 gaaatcctgt atttttttta ctatgtcttt aaaactggtt gcatcttcta aaggaactag
     1861 ctttagagta gtagcaaaag ctatcaagtg actatgtgaa tctataaaag atggcatcaa
     1921 tgtcttacct tgtaaatcta taatttctgt atctttattt ttcaaagcaa atacttcttc
     1981 ttttgttcct atctttttta ttattttgtc ttttataaga atagcatctc cacatattga
     2041 atcttccatt gtgatgatat ttccattgta ataaattttt gctttcataa aattctcctt
     2101 acgttgtcta ctttaataaa tttttttcat tattttctgc ttaattatat tatcttcttt
     2161 ataaactttt tctttagagt ttaatcatat aaaagtttta tttttaatta ttgtttgcca
     2221 agaagtaaat actattcatt gtaaataaga caaattaata ggctttatac taattgaaaa
     2281 aatattataa gttgttaaat ttaaatatct aaactcaatt atatctgtac aattgtaatt
     2341 ttttaaatca tgatgtatta ttttaaatat acagttacat tattattcac aatataagta
     2401 tcttagttct caaataacat ctcatgtaat ctattcaatg caaaaaattt aaatattttt
     2461 taggaggttt tttatgagca aggaaaataa aaatgtaaga attgctgttg tccaggcgtc
     2521 accagtcata atggatttag aaaaaactgt tgaaaaagca ttaggattaa ttaaagaagc
     2581 tggtagaaaa ggggcaaata tcgtagtttt ccctgaagca ttcatacctg catatccaag
     2641 aggtctttca tttggatttg ttgttggaag tagaacaatg gaaggtagag aagattggaa
     2701 aagatactat gataattctg taccagttcc tagtgctaca acagatttat taggaaaagc
     2761 agcacaagaa gcaggagtat atctatcaat gggtataaca gaaagagatg gtaatgatat
     2821 aaattgtacc ctttattgca caaacttatt tttctctcca gaaggtaagc taattggtaa
     2881 acacagaaaa cttaaaccta caggaacaga aagatgtatt tggggagaag gagatggaag
     2941 tactcttaca gtagttgata ctccatatgg aaaaatgggt tcgttgattt gttgggaaaa
     3001 ttatatgcca ttagctagaa cagctcttta tgctaagggt gtaaaacttt acatagcccc
     3061 tactgcagat tcaagagaag agtggcaagc aactatgaaa catattgctt tagaaggaag
     3121 atgttttgta attggatgca atcaatatgt tgaaaaaaat acgtacccaa ctgaccttaa
     3181 ctactataag gagctagatg cagaaccaga aattatgtgt ccaggaggaa gttgtatagt
     3241 tgaccctttt ggaaaatatg tagcaggtcc tattttcaat aaagaagaaa tgctaatagc
     3301 tgatttagac ctagaaaaaa tagtacttag tagactagat tttgattcag aaggtcacta
     3361 ttcaagacca gatgtatttg aattaattgt tcatgaataa tctttttaca caaacatcta
     3421 cataaatctt tttacataaa aaaatgagat actcataagt aaatttcaag tatctcattt
     3481 ttgcttaaaa cctataatcc taattataaa tttattcacc tatatttttt ctaaatcaac
     3541 tactgttctt ccaacatgtt ttcctgccat tattgaatct atactatcat ttaactcttc
     3601 caatgatact acatttacca tatcatctaa atcatctaat ttccattcat ttgataataa
     3661 atcccatatt tttaatctta agttcattgg gcaatccaca gaatctatac catataaagt
     3721 aattcccttt aagataaatg gatatactga acttgtaaaa tcaactcctc ctgcattacc
     3781 acaagttgtt actgctccac aataattagt tgtctttatt gctgttgcta gagtatttcc
     3841 tcctactgta tctattactc cagcccatct tggtctaagc agaggtcttc ctgagttatc
     3901 atccacttct gacctatgta taacatcttt aacacctaag tttagtaata cttctttttg
     3961 ttcttctaat tttcctgttg aaactacaac tgaataacct aatttaatca atattttagt
     4021 tgcaattaca gcaactcctc cagtaccacc agtgactaat acatctccca tattcttgtc
     4081 tacagaatct ataaatttat acacagataa agctgctgta aaccctgctg taccataatc
     4141 cattgcttct tttagtgaaa gatttttagg aagtttaact acccaactag aaggaactct
     4201 tatgtattct gaaaaaccac cagaagtatt cattcccaag tcataacctg ttactaaaac
     4261 ttcttctcca attttaaaat catcacaact actagattct actataccag atgcatcaat
     4321 tcctggtgta tgaggatact ttctcgtaac acccttatta ccatttgcag aaagtgcatc
     4381 cttgtaattt aaagaagaat attttacttt tattaaaact tcgccttctg gcaatgtatc
     4441 cacttctctt tcaactatat ttcttctaaa atttccttct tcttctgtaa tatatagtga
     4501 tttaaatttt ttattcataa atttcctccc aatacattta gttttatgtt aaactaatta
     4561 tagtttataa atagttttat gtcaaactaa tttaaataga ttaaagattg gagaataatt
     4621 tatggaagat aaatatattg tacatttcat aagtaaaaca aaggctagta tgattaaatt
     4681 cattgaaaat aaactcagta aaaatggttt aggtgagtta atacctactc atggaaatat
     4741 tttaactgca ctttatgaaa ataatggaat acttacaatg aaagaaattg cagctaaaat
     4801 aggaaaagat aaatccactg ttacagtgtt ggttaataaa cttataaacc taggatattt
     4861 agaaagacaa aaatgtacaa atgacaaacg tatcacgtat ataaagctta caaaaaaggc
     4921 actgcttata gaagatactt ttaatagcat ctcaacacaa gttaaagaga cggcatatca
     4981 taatattaca gaaaatgaga aacaagaatt tcttagaatt ttaaaaaaaa ttaatgataa
     5041 ttttaaaaat gctgactaaa ttgagcataa ttatatctcc tattggaata ataaataaaa
     5101 agaatcaaat attgtaaaaa tttattgtga tagagaggta gaatatgaat ttaaaagata
     5161 gtaaaactaa agaaaattta ttaagagctt ttgcaggtga gagtcaagct agaaatagat
     5221 acaatatttc tgcatctgta gcaaaaaaag aaggattata tataatagag tctttattta
     5281 attacactgc tgaccaagaa aaatcacatg caaaacaatt cttttctaaa ttaaaagaat
     5341 ttgctggtga aaatataagt atatcaggag cataccctgt agatttatat gacaagacag
     5401 cacaacacct taaagctgca caacataatg agcttcaaga gtgggatgat gtatataaaa
     5461 actttggaaa aacagcacgt gaagaaggat ttgaatctat agctaagtta tttgaaaata
     5521 tagctgaaat agaaaaagtt catggtgata gatttggtag atatgctact gatttagaag
     5581 aaggtacttt attcagaaga aatgaagata tacaatggat atgtacacat tgtggccata
     5641 tacatatagg taaagaagct ccaaaagctt gtcctgtatg tcttcatcca caaggatatt
     5701 tcttagattt taaaaactca ttatttgaat aaattaaggc aataattcta tttaaacaat
     5761 ttactatatt tatatcaagt atttttataa atactgtatt atcttaataa caataaaatg
     5821 aaccattgat attactataa gtagaaaatg tagtttaaat acaatttata gtaattcatc
     5881 atggttcatt ttattgttta ctaatcccta taacctactt aaatctatct ttaactttta
     5941 catactcaca taaaatctta tctttaaact aaatatttaa ataaagtgta ttttaagtat
     6001 attattataa tacattatat aagataatac actatgataa ctttatactt atatcattta
     6061 tttcttttgt caagtcaatc tcaataatct taactacatc tttttcaaac tttacttttt
     6121 tataatccag acatcctact acgaatggaa ctccaaactt tctaaacaaa taaatctttc
     6181 cactttcatt gtatattgat gtaaaatttt cttctattat attcactatc aattttaact
     6241 cttcaaaagt tattatagat ttaactaaaa acttctctat tatgctcaat ataagcagtt
     6301 cttttttacc tatgctttgc tcttttttat ataaattttg aaatatttct ttggtataat
     6361 tacatataac ttctttactg agaataaaat caatgtcaaa ttctttatta atatcctttt
     6421 tcataaacat atttgcaata tcatcaagag aagtctcttc tttcatagac aatattaact
     6481 ctattctttc tagtatttta tctcttggaa agaaagtctc ttgacctgta aaagctgatt
     6541 tttttataaa ccattcctct ggtattatgt tttttctttt ccatctatat aattgaccat
     6601 aagatatatt agttttgctc aataaatctt tcttagatat taattcttcc ataacttttc
     6661 tagcccacct ttatactgtt ttttacagta gaattatctg aaactgataa attatcactg
     6721 tattcaatat ttccaacatc acaattatct actagagata tgcttttacc tcgtatatct
     6781 tttattgatg catcttctaa ttcaatagta tctccctcta tcaaattaca tttaaacttt
     6841 cctgaaaaaa tattaatgat tttaattaat gaaaaagcat ctttgcttct tcctattttt
     6901 atattagttg cccctatctc tttacaataa gaacctcctc ctgaataaac atatatattt
     6961 tctccattta aaagaccatc acattttatt gctccatata cctttatatc ttcacattct
     7021 atatttgatt tatttctcat ttcgccatag atttttatat cttgtgcata catattttca
     7081 tttacactta attgtcctct tattgtaaca ttattacatt taagtttgcc atccactttc
     7141 tgttgagcag ttataactaa gtcatctatt tctgcatctc cctctatatt aagttgacca
     7201 ttcactctta cacgctctgc tttaagattg cctcttataa tagtttttcc gttaacaaca
     7261 aaatttttta tattgatgtc accttcaaat tttgcatctc caagaacttt tacttcgtta
     7321 cttttaacat ttccttttga tttagagctt cccacgattt ttatattttc atactcacca
     7381 ctacttataa agccttctcc aagtattttt atagaccctt tttccatatt attacctcat
     7441 tttaaatttt atttttaaac ctacttatta cttatccttc tttaatttta ttttttcact
     7501 tatattcttt atttcaccaa ctattgaatc ttcatcaatc tctatacttt cactgtactc
     7561 caaactttct acataacatc ccttgcctat tttaatattt tttcctctta caactttgac
     7621 ttcactattt tctaagctta tttcatctgc ttctataata tttgcaaaca atttactttc
     7681 tgtatacttt ctaattgata atagacctaa acttatcaaa tttgaagaca ctttttcaaa
     7741 gttgatatgc tttttttcta gcatacgaaa ttttttgcaa taatctccat gccccttttt
     7801 tatatttata ctacttgctc caacttcatt aaaattagaa gttccattta aactaatatt
     7861 aacttcttca caattcaaaa atccattaca ctccaagatt ccacttaaat tgatattttc
     7921 acaatctata ttctcatttg cttttaaata tccttctcca tgaataatat cagattttat
     7981 acttccttct acattaataa ttccactagt atcaatttca acagaattaa tatttcctgt
     8041 acatctaaga agtcccgata tctcaacttt tgatgccagt atgtttcctt ctactttact
     8101 taaaccagat gtaaaaaatt cattacattc tgttgtccca aaaacttttg ctgaaccaga
     8161 agaatcaaat ttactacatt ttacacttcc ttctattttg cctgttcctg atatattaac
     8221 attatcaaaa attccacctt tacattttcc aacaccatca aatcttagtg aacttaattc
     8281 ttcattcata atgatatcct ttctttaccg tattttatct ttaaacctaa tataacaaaa
     8341 cattgttacg ttgtcaagta tgataataat acttaataaa gcaactatca tttattttct
     8401 ataaataggc ataaaaataa tagggaaatg ctttatttaa actataaatc taatttctta
     8461 gattgtggtt taaataaaat atatccctat tactcaatct ttatatattc attgtataaa
     8521 cttctaaaat ataagtcttt aaattctact gtcaatactt tttatacact gtttgtgcta
     8581 tcatctcaca cacctaaagg agtgggcttt ccacactatt ctgtaaccta caagacacta
     8641 tcttcttcta tctattgata aatttttcta ttacatcagc tagtctctcc atatcagttg
     8701 gataaacttc acctatgcta cctattctaa atgtatctat atcagtcaat ttacctggat
     8761 agataacaaa tccattatct tttaaataag tataaaattc cataaattca aactttgcat
     8821 ttttaggata taagaaagtt gttattacag gtgattgagc attatcattc accaaagtat
     8881 caaatcctaa agattttaat cttgaagcta ttgtaaactg attttcttta tatctagcat
     8941 atcttttttc aacagaacct tcttcttcca attctaataa agcttgataa aaagctctta
     9001 caacatgtgt aggtgaggta aatctccatt taccattatt tttctccata gtctcccatt
     9061 gcgcatacac atcaagagat aatgatttag ctataccttt acatttagat aatttctctt
     9121 tattagctat tataaaacca aatccaggta ctccttgtat acatttgtta gaactactaa
     9181 ctaaaaaatc tatattaaaa tcttcaacat caatctcaat tcccccaaaa ctactcatag
     9241 catcaactat ataaattttg ttatattctt ttgcaagttt ccctacctct tgtataggat
     9301 ttaatctacc tgtagtagtt tcacagtgaa ccattgatat atgtgttata tccttgtttt
     9361 cttttaataa attttctact acatttaaat ccactgcttc tatatcttta aaagtgcagt
     9421 caacaaattg tatgttaaga taattacata tatctttcat tctctttccg tatgctccat
     9481 ttgcaattac aagaagttta ccatcttttg aaatagtact tcctattata gcttctactg
     9541 aaaaagtccc acttccttgc attaatacac ttgtgtattt atctgtattt tgggttgcta
     9601 gagatactaa tcttcttcta acatcttgta ctaggttatt atattcaaca tcccaagtac
     9661 accaatcttt taacatagat gctcttactg atttagtagt agatagtggt cctggtgtaa
     9721 gtaatatgta atcattttct ggtacaaaat ctgatttaat agtttcattc tctattttta
     9781 ttagtatatc ttctaactca gccatagttt ctattacaaa atgtgctcct gcctctttaa
     9841 atttctttga aactatactc atcttagctt ttaattcttc cttatccatg ttctctactt
     9901 cttcttgtgt aagccctaac tcacttgaac cttttattac agcaacactc cacattcctg
     9961 catttacgcc ttcttttaca tcacttattg tatctcctac ttttaccata gaactcatag
    10021 gagacacccc taaagcttca gcatttttat agcacatcca aggatatggt cttccttgag
    10081 atacttctga tggtgttaca agaaaatcgg gtgagtatcc tttctttgct gcatttggtt
    10141 caactatgtt catcatctct cttgtataac ccgttgtaga acctattttt aatccatttt
    10201 ttctcaactt ttcaatagtt tcaacaacat gaggaattgg agtcgtatac tcttctaatg
    10261 tctcaaataa cattggctca aattcagcat aaagttcatt tacatcatct tcagttggca
    10321 ctttaccaaa cttatctgac caaatattct ttattctatc catttcacac atttctctta
    10381 tatggtctat ttttaatttt cccataggtt ttcttgcttc ttccattgtt acatctatac
    10441 ctcttctttt aaatatctct ataaatacat taagaggtgc aaaacatcca taatctactg
    10501 ttgttccagc ccaatcgaat actactgctt ttattttttc tccatatatc tttttcatat
    10561 ttatagcctc catagattct ttttagaatt tttgtgtttt taattgtttt tacttttaaa
    10621 gtataacttt catatgttaa ttatacatca tatttaggtt aaatatatat taaatttctg
    10681 tttttagttg tatttagttg tttttatttt gtatcatatt cccataaaat cctaagttac
    10741 aatctaaaat ttactttcta atcttaatct atgaactgac tatattttat gaatttagtt
    10801 gtattgagca tagttgtatt atataaaact aagggtttat tttgcttcta tccatgttat
    10861 atttatctca tttaacttat ccttccattc attcggaggt tctacatcac aaataacaaa
    10921 atctaattcc tctaaatctg ctatcttata catactagca attcctattt tagaactatc
    10981 acataataca aaactttcct ttgaattttt tatatatgct tttgacaaca atgccttatt
    11041 ttggtctata ctactaattc caaaactatc aagtatacct tcactagata taaaagcttt
    11101 atctacgtat aagtttttca tagcatctat tgatattgga catgctgtcc tttggtgttt
    11161 taaatttact tttccaccaa taaatataac ttctccatca aacaaacctt gattctcgta
    11221 agaaatcaat tttgacagaa ctggataaga gtttgtaata atttttaaat tgtttacaga
    11281 tattatataa tctacaatct gcaaattggt agtaccttca tctatgataa ttgactcatt
    11341 gtcatttaca aacttagctg catatttagc aatactttgc tttttatcta ggttagtatt
    11401 gtttctcttt atatgttctg gttcaatcac tccataattt atttttatag caccaccata
    11461 tacctttttt aatttatttt cattttctaa ttcttccaaa tatcttctta ttgtttctga
    11521 tgatacttct aatcttttta ctaattcttt agtgtttacc ttcccttttg aatctagtaa
    11581 tttaagtatt tctttttttc tatctatcga atttaaagac attcattttc ctcctaaata
    11641 taatatattt ttctcctata atattacaat gatacctaag atttatgaaa tcatatttat
    11701 gtattaaatt ttttacttaa ttaaagttat tttcaaaaga ctttgtatta tcattaaaaa
    11761 agtccatcaa atattagtat cagatggact ttgtaaaatt ttattttata aattaatatt
    11821 taaagtctaa ctactaattt attttcttgt ctccaagaat tttactaaat tatttacagt
    11881 agacatatct tttctctcta aatcagttgg ttgaagctct atccctaatc tattttcaat
    11941 ttcaagtaaa acttcaatta tagctaatga gtctaaaagt tccgtctcaa ataaattaag
    12001 gtctaaatct tctcttatct catcagtccc taatacatct tcaaatattt ctataactgt
    12061 ttcttgcata aattttcctc cttaaaatta agctaaataa cctgagaata tcagcaatcc
    12121 aaaacacaca atatggaatg taatgaaaat ctgcactctc tcgaaccatt tatctttttt
    12181 atgtttttta taaaatttag attttcgctg atatatatca gttaacacaa gtgccaatcc
    12241 ttggtataca ccataagcaa cataatacca agttagacca tgccaaaatc ccattgttat
    12301 catggttatc atctgagcca catgagcagc agtagtcctt ttcttaaatc ttttctttct
    12361 cattgatgac ataacaaatc tagaaaaaat ataatctcca aaccatcttg aaagacttat
    12421 atgccatctt gtccagaact ctttcatatc tttgctgata aatggcttgt caaagtttat
    12481 aggtacttgt attccaaata tgtaccctgt acctatagca aataaactat atcctgcaaa
    12541 atcaaagaac aggtataaac tatatgcgta catataactt aaaatattta taaaactcat
    12601 atctttaggt attcttgata cccaatatgt atttatcaaa aatgctataa caaacttata
    12661 acctactccc atgacaatat tttttattcc tggcagtaag tattcttcta tatactcttt
    12721 tctagagatt tgtttttcca agtcctgttc aaatcttctt gacctatcaa taggtccaga
    12781 acttaaagtt ggaaaaaata acataaaata aagcatcttt gaaattttaa cttcttttat
    12841 agctccatca taaatttcaa taaccatttg tatagttctg aaatttaagt atgatattcc
    12901 aataaatcca attattccaa aagaagtaac tggagatatt ttatttatta ttattgggag
    12961 cattgatgca aacaagaatc cccaatatat gtatttattt ttagtttttc ttcttacata
    13021 ttcatatcct ttaactatta ttacttcaag aataataaat attaccaagt actttagttg
    13081 gacatcaatt cctactatta agaatatcat aaacaaactt gccaacatac cataatactt
    13141 tatattcttg cccattagac ctaaaattac tgctggtatt gatgttaata ataatatata
    13201 aagataaaaa taatctccat attgtgaaaa aatcattaaa tttcctccat aagtttcttt
    13261 ctgtctattt taccattgat atttgttgga aactcagaga ttattgaaat atttcttgga
    13321 atcatatatg aaggtatata ctttccaagt tcctttttta ttatcatccc atttttaata
    13381 ttacttaaat cattcttttc atttaactca acaatacctt ttaagtaagc aattttttcg
    13441 tccttatata ctggtaaaac tactgcattt tttacattat gtacttttct caaattattt
    13501 tctatatctt ctatttcaat tctaaatcca tttaatttaa tctgaaaatc ttttcttcca
    13561 caataatata tatttccatc taaaagatat cccatatctc cagttttata agctctccac
    13621 tttacaccat ctatttcatc atagaaaaat acttcatcag tcttttcttt attattgaag
    13681 taacctttag aaactgatgg tccaattatt ataatttcac ccttttcatt ttctttaagc
    13741 tcatttccat cttcatcaag tatttttatt ttacaatttg acatagggta tccaactgga
    13801 agactttttt catcatctat agccttttga gtcatatcat ttacacttac accaacagta
    13861 gcctcggttg gtccatatcc attgataatt cttgtgtttg gaaatctact cataagttct
    13921 ttagtaagat tttttgaaag tgcttctcct atgaatatca ttgactcaag gttaggaagc
    13981 attttactat taaactcttt ttcagttata catactcctg caaaagaagg agttgacacc
    14041 catacagcta tatctgagat actaaattgt ctaaatagtt ctttatagtc tgctaataca
    14101 tcttttgata ttgaaaataa tgtagctcca tggataagac cagggtatat tgttgtaact
    14161 gaaaggtcaa aagaatatgc tggttggttc attattactt tttcacttcc atctatgttt
    14221 agatatgggc ttatccaatc tgaaaagcta tctaaattgt ttgaacttat ttgaacccct
    14281 tttggctttc ctgtacttcc tgaagtaaat agtatatatg cattttcatc atcttttacc
    14341 cagttttctt tatctaatga ttttccctga tattcattta ttatataatt taatttatcc
    14401 atatcaatta cattgatatc tccaaaattt ctttcatcac taaagttaaa taataccttt
    14461 ggttttatct ctttagtgac ttcaaaaact ctatcaattg gaaaactaat atcaagaggc
    14521 acatacgctc tacctgattt taaagctcct atcatacatg ccattatcat gttttcttta
    14581 tttccataaa ttactatagg agtatcttct tctttgtata catcctttag aaatactgat
    14641 atagcatctg aatattcatt taaatcttta taagaaagtt tatctccatt acacattagt
    14701 gctgttctat ctgtatttga atattttttt attccttcaa taattttcat ataagaatgc
    14761 tcctttaaaa tccgttataa ataaatggca attctttaaa tggagtaaat gttagaactc
    14821 ctatcgccaa tattataacc atagttaaac aaatactagt taacaaatct ttattgttct
    14881 ttaaatattt taaataatct ctcacagaca tctacccatc ctttcgtacc aagatgcatt
    14941 acatccctta aataatactt atctgaccct ttatctttaa ggtttagaac ttcaaagcct
    15001 ttgctttctg ctattttttg tgctttatcg tagtactgat ttctttcttt ttcagaaata
    15061 ccagtcaaat tatagaattt atccatactt gggattaata caacaactgg cttaatacct
    15121 aagtcagtac atacatttag catcagttta taattttcaa actccttaga tgttaataaa
    15181 tttacatctt tgtctcttcc tttgtattgc tctataccat ctttaaaatg ctctttataa
    15241 tagtacttat caatatttag tggattttta ccaactcttt ttttagcatc ttcaatggct
    15301 ttctttctct catatgacca atttattggt ctttttcttt ttgttgctct ctttttatct
    15361 agcttaatta atcgtttata aagaataccc ttttctttaa gagctataca atactttctt
    15421 ccttgaaaat aaggctctaa aagcaccttt tcagcttttt caggaaatgt cttaggctca
    15481 tatagcttag catataatgc ttctgcttta tactcgtcac ttccccataa taacttactt
    15541 gatttttttg catattcaat cttattttgt ttagatattt ttggattatc taaaaatcga
    15601 taaaattgta ttggtgaaaa tctagactga taatggtgac ttgtgactcc gtctttttcc
    15661 ataaaccatt gcatagaaat taacaagaca actttcttat tatcaatatt aggattcata
    15721 cttcctaata ttgcagcatc ttgtaatgtc tgagtatagg ctctcccaat tgcaaacacc
    15781 ttatttttac tcctatttgt attaaaatag tactttggat gttgttttgt tgaattacta
    15841 agttctgatg accccaaaat cataatatcc ttttctctta aaaaatgatt atttgctgta
    15901 acccctttat cttttatatc acttaaagta tcatccataa ttggaagtag atttttctcc
    15961 ctcaaaagtt catcagtttt agaatcaaga aatttgtcta acccaaataa aaatactacg
    16021 cctattataa aaggtgtaat aaagtatatc aattttctca tattttctct cctctaaaaa
    16081 tattcaaatt tatgttatta ttaatcatat tagttttgat atgtatattc aactacaaaa
    16141 ttgtaataaa atcgtcaaaa ctttttttga tactgttaca aaattgtaaa aaaaaagaaa
    16201 aaaacgccaa aaataaattt ctttttagca ctttttcata cttataattt gacattttaa
    16261 cacttggtta agtatacacc ttaccttaac ttaaaattaa atttatttaa gaatatatat
    16321 tataaattct tatattttct aatataaact atatactatc agctggataa acaatactac
    16381 tttgtcttct tatctcatcc ataatttcca ttactattat tgaattttca tgagaattta
    16441 tatttgattc tattttattg ttatttataa gacttataaa ctctacaagt tcataataca
    16501 ttccttctat ttctctaggc ttttcctctg ctttttcttt tggaatagat atatcttctt
    16561 ctcttccatc tctgtattta ataatagctt tttcaaatag attaagcttt tcaattatta
    16621 tacttccctc ttctccctgt atttcagatg gtatataaga atcagctact ttagaatact
    16681 gaattatcgc atccatatca tcatattgaa atacagctga accttctcca tccactccag
    16741 tttgaagtat atgagaaact gcctttacag tttttggagc accaaataaa ttcaccatag
    16801 gatgtataca atacactcct atatccatta aagctccatt tgacaactct tttttaaagg
    16861 cattttctat aattccattt ttatatttat catatcttga agaatactga caatagcttc
    16921 caaaaaatct tcttattttc cctattttat acaagttttc tttaactgct ttaaagtttg
    16981 gaacacatgt aattctcata gcttccataa gcaatacatt attatctttg gctgttttta
    17041 tcattgattt gacttctttt aaattagatg ctaacgactt ttcacaaagc acatgttttt
    17101 tgtgttttaa acaagtaatg gtttgctttg aatgtaatgc atttggagaa gctatgtata
    17161 cagcatctat atccttcgat ttagccattt gttctaaatc atcaaaaaaa atacttgctc
    17221 catatagagt tccaaattct ctagcttttt ctaaatttct agaatataca gcaaccaatt
    17281 caaagtcttt aaccctagat gcagctctta gaaatatatt tgctatattg cttgtcccaa
    17341 caataccaaa tcttatttta cccatcataa atcactcccc attttctaca ctttatttta
    17401 attttaataa aaaactttac aacttataga tatattataa aataatttta tcaaataaaa
    17461 aagaaagcta attttaaatg atttcaccat tttaacatag ctttctttta actacaatct
    17521 aaattcaact tatattttta aagttatctt tctactatat ctttatttcc cctcaaaaat
    17581 tttatagtaa gaatttcatc cacaaacaat gttactaaaa gtatagttac aacaattggc
    17641 caataaggta ttataagact atataaacta tcaactatat acacttgaaa atatctaact
    17701 ccaataaatg tcaatatagt ccaatataca gaaaacttta gacatactat tcctctacta
    17761 ttgtacttat aatctgaata atcccaatag accttattaa aatatacttt cataagtatt
    17821 cctgtaatat attctactac tgtcggcact actagacata ataaaagcaa tgcaaatata
    17881 ttattggggt caataaatct tgagacctct attataatcg acattgcaat tgcatacatt
    17941 ggtttaaatg gaccatttaa aaatccatct tcttgaaaat gcccttgttt ataataacta
    18001 tataagtttt caattatcca acctataaaa ccatacaaga aaaaattaaa taaaaaatat
    18061 aatagagaac ctacattatc catgaatatt cctcctagct gtttatactt attttttctc
    18121 atattcatgg atttattcta aatcaaagaa attatattta atttatttaa ttaatacttt
    18181 atattataga ttgtgacatt ctttagagtt caaacttttt aggtattctc taacttccag
    18241 taaattttca agtatcttat gtgattgtga tttaatttgc tcatctggct cttttaaatc
    18301 tggtacattt atacatctta taccaccatt gtatgcagct tcaactccca taggcgaatc
    18361 ctctatgaca atacaatttt tgggattaac atttatcttt ttagctgctt ttaggaatat
    18421 ctcagggttt ggtttcgagt ttacaacatc atctccacaa actatagcat caaagtaatc
    18481 ttttaaattt gccttagcta atctctttac tgctctttct ctctttgttg atgttgcaac
    18541 agccattttg tatccatttt cctttaaaaa agagattagt tcattgactc ctaatttgat
    18601 aggagctccc ttcctctcca taaattcaat catattttta gttttttcgt catataaatc
    18661 tattattggt acagaactat cgtatatatc tgtaagacct tctattattc ctttacgatt
    18721 tcttcccata actgatgtat aaatttcctt agtcatagta tatccatact tttcaaacgt
    18781 ctccatccaa aattcaagag aaattctctc tgagtcaaac aaaactccat ccatgtcaaa
    18841 aataattcct tctacttttt gcatacaatt cctccaatat tttttgatat aattatatta
    18901 cactttttat gattaatttt acaaccctca atttatccaa ttctaatatt gtaaaattgt
    18961 aattttcaaa agttacactt tggtttactt taatatttga accaagcata taggatatcc
    19021 atccacctat actgtcaaat ccatcatgct ctatatcaag tccaaaatac ttattaatat
    19081 agttgataga ttcagttcca tcaactaagt aagaacctgc tcctattttc ttaatatgag
    19141 tctcttcttt atcaaattca tcttgaattt ctccaactat ctcttctagt atatcttcta
    19201 ttgttaccac accagatgta ccaccatatt catccacaac tatagcaagt tgaagttttt
    19261 cctttcttat tctctcaaga gctttatcta tagttaaatt ttcagatata tatataatat
    19321 ctctcaatat ttcttctagt tcaattttat tttcatttat cttttgattg tataaatctc
    19381 ttatatgtac aaaacctaat atatcatctt tattttttct gcatactgga tatcttgtaa
    19441 caccttcctc ttttaaaata gctaatatct tttcttcaga atcactttca tatatacata
    19501 ccatatcagt tctaggaacc attatctctc taattttctt ttcttcaaat tcaaaaatat
    19561 tatcaactaa tctttgctct gattcatcta taagtccact tttataactt tcttctacta
    19621 atagtcttat ttcgtcacct gtatggggtt catcaacctc atcagcttgt gaatatccaa
    19681 atggtttaag aaataaatca gtacttaagt tgaaaaaata tattattgga tatgttaact
    19741 tataaaatcc tactaataaa aaagatgtac tcaacattat tttttctgta ttataaagtg
    19801 ctaaagcttt aggaaccaac tctcctatta caacctccat cattgttata attaaaaaag
    19861 aaatggaaat tgatatagta tatataatac tttcactaag attaaataca cttactattg
    19921 gtaaaattaa ctctttaact gtagattctc ccatccaacc taatgcaagt gaacaaagtg
    19981 ttataccaag ttgacatgca gaaagatagg aatttaagtt gtcttttatt attttacatc
    20041 tttttgcatt attattacct tcagttatca tggtatcaat cctagaacta cgaagtttta
    20101 ccatagcaaa ttcacaagct acaaaaaaag catttatcaa aacaagcatt actataagtg
    20161 ttacttttaa taactcccgc ataaagtata ggttcttata atgtaagaaa tctataagaa
    20221 ctcaaagttc cccccttata ttaataaatt taatttataa tatatattct atcatattta
    20281 ttaat
//
LOCUS       DAMJWS010000078        20167 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMJWS010000078 DAMJWS010000000
VERSION     DAMJWS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.079"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..601
                     /locus_tag="PC399_002873"
     CDS             <1..601
                     /locus_tag="PC399_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2593721.1"
                     /translation="VFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQ
                     RELSRKTIGSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKN
                     MIRNRKLSRLISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVK
                     DLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            719..1024
                     /locus_tag="PC399_002874"
                     /pseudo
     CDS             719..1024
                     /locus_tag="PC399_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            1037..1942
                     /gene="truB"
                     /locus_tag="PC399_002875"
     CDS             1037..1942
                     /gene="truB"
                     /locus_tag="PC399_002875"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF2593722.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            2073..3002
                     /locus_tag="PC399_002876"
     CDS             2073..3002
                     /locus_tag="PC399_002876"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF2593723.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3106..3363
                     /gene="rpsO"
                     /locus_tag="PC399_002877"
     CDS             3106..3363
                     /gene="rpsO"
                     /locus_tag="PC399_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF2593724.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3603..4448
                     /locus_tag="PC399_002878"
     CDS             3603..4448
                     /locus_tag="PC399_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2593725.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4573..6684
                     /gene="pnp"
                     /locus_tag="PC399_002879"
     CDS             4573..6684
                     /gene="pnp"
                     /locus_tag="PC399_002879"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF2593726.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            6950..7693
                     /locus_tag="PC399_002880"
     CDS             6950..7693
                     /locus_tag="PC399_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2593727.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7851..9098
                     /locus_tag="PC399_002881"
     CDS             7851..9098
                     /locus_tag="PC399_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2593728.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGI
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9133..9378
                     /locus_tag="PC399_002882"
     CDS             9133..9378
                     /locus_tag="PC399_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2593729.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGDILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9388..10611
                     /gene="dapG"
                     /locus_tag="PC399_002883"
     CDS             9388..10611
                     /gene="dapG"
                     /locus_tag="PC399_002883"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2593730.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10724..11458
                     /locus_tag="PC399_002884"
     CDS             10724..11458
                     /locus_tag="PC399_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF2593731.1"
                     /translation="MKDYREDNEKFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11581..13992
                     /locus_tag="PC399_002885"
     CDS             11581..13992
                     /locus_tag="PC399_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2593732.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13995..15053
                     /gene="mnmH"
                     /locus_tag="PC399_002886"
     CDS             13995..15053
                     /gene="mnmH"
                     /locus_tag="PC399_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF2593733.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            15047..16381
                     /gene="rimO"
                     /locus_tag="PC399_002887"
     CDS             15047..16381
                     /gene="rimO"
                     /locus_tag="PC399_002887"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF2593734.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16368..16910
                     /gene="pgsA"
                     /locus_tag="PC399_002888"
     CDS             16368..16910
                     /gene="pgsA"
                     /locus_tag="PC399_002888"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF2593735.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17198..18244
                     /gene="recA"
                     /locus_tag="PC399_002889"
     CDS             17198..18244
                     /gene="recA"
                     /locus_tag="PC399_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF2593736.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGISVSKEIVEE"
     gene            18420..19961
                     /gene="rny"
                     /locus_tag="PC399_002890"
     CDS             18420..19961
                     /gene="rny"
                     /locus_tag="PC399_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF2593737.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8254 a         2043 c         3900 g         5970 t
ORIGIN      
        1 agtatttgaa aatactaata atcatatagg tttagattta ggaattaaag aattttgtat
       61 ctcaagttgt ggagacttta tagaaaatcc taagtatctt aagaagtcat taaacaagct
      121 tgctaaatta caaagagaat tatcgagaaa aacaatcggt agtttaaata gaaataaagc
      181 aagattaaaa gttgcaggac ttcaagaaca tatagctaat caaagaaatg attttctaca
      241 aaaactatct actaaattaa taaaagagaa tgatattatt tgcatagaag atttacaagt
      301 aaagaatatg attagaaatc gtaaactatc tcgcttaatt tcagatgtat catggtcaga
      361 atttattcgt caattagaat acaaggctaa ttggtatggt agaaaaattg taaaggtagg
      421 taaattcttt gcaagttcac aaatatgcaa taaatgtggg tataaaaacg aggaagttaa
      481 ggatttaaat ataagagaat ggatttgtcc tagttgtaat gaaactcacg atagagatat
      541 aaatgcaagt ataaatatat taaaagaagg attaagacta ataacgattc aaaataaata
      601 acttatgtaa gaaccgtagg aactacgggg atagcctgtg gagaattagt aagacatatt
      661 ttaatatgca aaattctgtg aagcaggaac cctgtgactt tagtcatggg aggttcagtc
      721 aaaggtatct agtatagttt taactcaaga catgatgaat agaaataata ttagctttaa
      781 tgatgttgat ggaataacaa catatacaag agacataaaa ggtatagaag ttggaatttt
      841 atttaaagag aaaaaacaaa atgaaattaa agtgagtttt aggtcaaaaa attatgttga
      901 tgtaagtgaa atagccaagt tatttggtgg tggtggtcat gtaagggctg ctggatgtac
      961 tattagagat agtattgata atgcaaagaa aatggtacta gaggcagtgt taaaatcaat
     1021 ttaaggtgac agtaaaatga ataagattat aagtatattg aaacccacag gtatgacatc
     1081 tcatgatgtt gtaagtagag tgagaaagat tttaaatata aaaaaagttg gtcatacagg
     1141 tactcttgac ccagatgctt caggtgtttt accaatctgt atagggaaag ctacaaaagt
     1201 atgtgaagtt attttaaaca aagataaatc gtatatatgt gagcttactt taggtataag
     1261 tacagatact tatgatgctt caggtgaaat tttaaaaaaa gttgatgatt ttaaatttag
     1321 taatgaagac atagaaagag cttttgacac acaaagagga gagataaatc aacttcctcc
     1381 aatttattca gctttaaagg taaatggaaa gagaatgtgt gatttagtaa gaagtggtag
     1441 acaatctgaa attactttaa aaacaagaag agtaaatata aaagacataa aaatactaag
     1501 tataaaaggt aataaagtaa tgttttatgt tgagtgttct aaagggactt atgtgagaag
     1561 catttgccat gatataggag aatatctagg ttgtggagct catatgtcgt ttttgaatag
     1621 aacatcttct ggtaaatttg acttagataa ttcaataaca ttagaagaat tagaattatt
     1681 ttatgaaaat aaaactttag acaaatactt atatgatata gattatgtcc ttgattcgtt
     1741 taattatgtt gtattaaacc ctaatgctat aaaatattat agtaatggtg gctcaataga
     1801 tgataaaagg tttttaaaaa ataatttcga taaagatgat gaatttgtaa gagtatacag
     1861 tacagataat ttcttaggat taggaaaatt atcaaaacac aataatacaa taagtgttaa
     1921 aagtgataag atgttcatat aaccaatgta tttaaaaaaa caacaaaaga gatagtacta
     1981 taataacttc tatagtctat ctcttttact tatgctataa tagtttgtga ataagttaag
     2041 agtaaattat attttagcgg ggaacattta ttatggtgat tataaaatct ataaaagaaa
     2101 tagcaaacat aaaagaaagt gtcattacca taggaaattt tgatggtgta cacaaaggac
     2161 atcaagttct tataggaaaa acagttgaac atgcaaaaaa agaaaatata cagagtatcg
     2221 tatttacatt tgcaaatcat cctgtaaatt actttagacc aaactcaaca aaaaatatta
     2281 taagcaatga tgataaaata aaattattag aagatttggg aatagatata gttgtaatca
     2341 taccatttga tgagtatatg actaaaatac ctgcaaagga tttcgtagat gagatactag
     2401 taaaaaaatt aaaagctaaa aagatagtaa ttggacatga ctttacattt gcaagggcaa
     2461 aagaaggcaa tgttaatcta ttaaaaagct tagaacatga atttggtttt gaggtagaag
     2521 taataaaacc catcaaaatc aatgatgtaa gagtgagtag tacttatatt agaagtttag
     2581 tatcccaggg tgatatggct aatgtcaagg aatatttggg tagaaattac aagttagaag
     2641 gttgtgttat acattctaaa caccttggaa gaactatagg attcccaacg gcaaatattg
     2701 accttaagaa taatatgctc gttccaaaga gaggtattta tgcttctatt gtacatattg
     2761 gagatgagac ttattttggt gctacaaatg taggctataa cccaacagtt aatggaaaaa
     2821 ctttgtctat tgaaacaaat attttagagt ttgatagaga tatttatggg gaaaatataa
     2881 ttgttgaatt tttggaaaga ataagagatg aaagaaagtt taattcaata gatgacttaa
     2941 aaaatcaatt gtataatgat acaaattatg tgtatgaaaa ctatgtttgc aaaaacaagt
     3001 aatgtatgat aaaatgatta aggaaaataa ccattactca gcatttgaat actccaatca
     3061 atgcttagta ataggcgttt ctaaaaaaat acggaggtaa tctaaatgat aaacaaatca
     3121 gaaataataa aagaatatgg aagagctgaa ggggatactg gttctccaga agtacaaata
     3181 gctttattaa cagctagaat aaacgagtta aatggacatt taaaaactca taagaaagac
     3241 caccattcaa gaagaggact attaaaaatg gttggtagaa gaagaaactt attagcttac
     3301 ttaaaggaaa aagatcttga aggatataga gcattaatag ctaaattagg attaagaaag
     3361 taatttcatt ctttataaaa aagcaggtat agtgtatacc tgctttttta tagttaattc
     3421 attataaagt aaaatagtta ataataaaag tagtttatat gttataaaaa attataaaat
     3481 gaataatatt gggtaagaat attaaaatag tatacaaata cataatcaat ttaaaaggaa
     3541 agacaggata aatatagaaa ttttataaca ggataattag ttggaataag gaggatatta
     3601 ttttgaaaat aaagttagta tgcgatagtt tgtgcgatat tccagatgaa atatcagaaa
     3661 aagattatat agagatagtt ccacttacag ttatttttaa tgatagggaa tatatagaag
     3721 gagtagatat aaagaaagaa gagttttata aaaaagtaaa agaaatcaag caaataccta
     3781 aaacttcaca agctacatat atggagttta aagaagtgtt tgataagttc ataacagaag
     3841 gttatcatat aatctgtatg acaggggctg caaacgcatc aggaactttt caaagtgcta
     3901 tgatagctaa aaatgatgta aatgaaaatg aaaaaataca tatatttgat actagaaatt
     3961 tatctctagg tagtggacaa tatgttataa aggcatgtga acttcttgaa gaaggactgg
     4021 gatttgaaga aattatagat gaattagaaa atactcgcag tagtgtaaga ttgctttttg
     4081 caccatatac acttgatttt ttaaagcaga gtggaagagt tccagttgca actgctttga
     4141 taggaaatat gttaaatata aagcctatat ttttctttga taatggggaa gctaagttgg
     4201 taaataaggt tagaggaata aagaatatag caagtaaatt ggtcgatata attttagaaa
     4261 tgaatgaagg gtctttagaa ggaaaaatcg tcacaatagg ttgtggagat aatttacacg
     4321 attgtgaaat attaaaagat gaagtaaata aaagaataaa agcaagaaga gtattattta
     4381 ctcgaggagg agtttctatt tgctctcata cagggccaga tatattggct ataagttgtt
     4441 ctaattagta gtaatataag gtttttgcat atatatgtga agataatttt atattgttaa
     4501 tttatagtat ttttgataat atagtaaata gagtgatata cgtgtttttt aataagagga
     4561 ggacaagcat taatgtttga acataaaata tttaaaatgg attttgcagg aagagaactt
     4621 tctgtagaaa taggaaagat ttgtgaaatg gctagtggta gttgtatagt tcgatacagt
     4681 gatagtatgg taatggtaaa tacaactaaa tcagccaaac ctagagacgg aatagacttt
     4741 tttcctttaa gtgtggatta tgaagaaaaa ttatactctg taggtaagat acctggaggt
     4801 tttttaaaga gagaaggcaa gccttcagag aaagctatac ttacatcaag attgatagat
     4861 agaccaataa gacctttatt cccaaaagga tttagaaatg atgttcaggt agttgcaaca
     4921 gttctatcag tagaccaaga ttgtacacca gatatagtag caatgatagg ttcttcaata
     4981 gccctttcta tatcagacat accatttaat ggaccaacag gttcagtatg tgttggcctt
     5041 gtagatggtg catttgttgt aaatccaaac gcagagcaaa gagaaaaaag ttcaatgcat
     5101 ctagttgttt caggtacaaa ggaagcaata atgatggttg aagccggagc tgatgaagtt
     5161 ccagacgagg taatgcttga tgctatttta tttgctcacc aagaaataaa aaaaatagtt
     5221 gagtttatag aaggtatagt tgcagaagtt ggaaaagaaa aaatgcctgt tgaattatac
     5281 catgcaggag aagaaataac tcaattggtt cgcgaatttg caactgataa gatgaaaaaa
     5341 gcagttcaga cttttgaaaa gttagagaga atggaaaata tggatagagt taaagaagaa
     5401 actttagctc actttgaaga aactttagaa gattttgaag attttgttgg agatatagaa
     5461 gaagtattac aagatataat aaaagaagaa gtaagaaaac ttatagtaca tgaaaatgta
     5521 agaccagata atagaaaact tgaagaaata agacctatat ggtgtgaaac tggcatgata
     5581 cctagagctc atggttcagc catatttact agaggacaaa ctcaagtact taatgttgct
     5641 actttaggtg ctttaggaga tgttcaaaaa ctagatgggt tagatgaaga agaaaacaag
     5701 agatatatgc atcattataa tttccctgca tatagtgttg gagaagctag accatcaaga
     5761 ggaccaggaa gaagagaaat aggacatggt gctttagcag aaagagcttt attaccagta
     5821 ataccatctc aagaagaatt tccttatgct attagattag tttctgaagt attaagttca
     5881 aatggttcta catctcaagc aagtgtatgt ggctcaacat tatctttact tgatgcaggt
     5941 gtacctataa aagatatggt tgcaggtata gcaatgggac ttataaagca tgatggtaag
     6001 gttgcagtgc tttcagatat acaaggtatg gaagaccatt taggcgatat ggactttaaa
     6061 gtagctggaa cagagtatgg tataacagca atacaaatgg atataaaaat tgatggtata
     6121 gataaagaaa ttctacaacg tgctttaaaa caagctaaag agggtagaat ccatatacta
     6181 ggagagatga gaaaaactat atctcagcca aaaccagaac tttcaccata tgcacctaag
     6241 attgttaaaa tgcaaataaa tcctgacaag ataaaagatg taataggacc tggtggaaag
     6301 ataataacta agataataga cgaaactgga gttaagatag atattgaaca aactggggaa
     6361 gtatttatat caggtattga gattgatatg attaagaaag ctcaagaact tataaataat
     6421 atagtagttg aaccagaagt aggaaaaact tataaaggta aggtaagtag aataatgaac
     6481 tttggagcat ttgttgagat acttccagga aaagaaggat tacttcatat atctcatata
     6541 gctcatgaaa gagttgccaa agttgaagat gtattgaata taggggatga agttgaagtt
     6601 aaagttacag agattgatga aaaaggaaga gttaatttat caagaaaagt tcttttacca
     6661 aaaccagaac ataaaaataa ataacttatt atataattaa aagcaaagtc ttaggatttt
     6721 gcttttttaa atacaaaaaa gtagataatt ttaaaatgat agttatggat ggcttatagt
     6781 tctttttctg tcaggcttta ctcttataaa gaagctattt aaaagctgat taatagagag
     6841 ctttattgat atatatagaa tcacttaagt tatatttagt gcataaacag agcttatttt
     6901 taatagtatt aaataagaaa caatttgtac ataattaggg ggagttttaa tgataatgat
     6961 ggtacttaat aagagtaagc tcaaaaaaat cataatacta attttagtag tgttattagt
     7021 attatcagga ggggtaatgg tattaaagtt atttaacaac aagccagtat ttaatttatt
     7081 taatggtgta gatttaccat atgaaagagg tacaaaggat aaaagtggat atgttgcatt
     7141 aacctgtaat atagaccttg gatgggaaac agagtacgta gaatccattt tagaaacact
     7201 taaaaaagaa aatgtaaaga ttacatttaa tgtaactgga aaatgggcag aaaagaataa
     7261 ggatgaatta ttaaagataa aaaaacaagg acatgaaata ggtaatcatg gatacaaaca
     7321 tttagattat tctacattat catatgaaga caattatgag caaatagaga cctctaaaaa
     7381 gataattgaa gaaattatag gtgaaaaaac taaatttttt caagcaccag caggttcttt
     7441 tggtccagaa acagtaaagg ctgctaaagc attagggtat acttctatca aatgggatgc
     7501 cgatactata gattggaaat acaaagacca accagaggta atcattgata gaatgaagaa
     7561 aaaagacatt aaagatagta gtataatttt aatgcatcca accaacgcaa cgacaaaatg
     7621 tatagatgat attatagcta ttgtaagaga aaaaggacta aaaccaggaa aacttagtga
     7681 tgtatttaag tagacaaagt ataaatattt ggttaagatt gtataagttg tagttgaatt
     7741 aattaaaaga ctgacttata atgtaataca tataaaaaat aaatataatt aaaacatagt
     7801 gttttcaata atccaatatg aaacaaagaa taaattgtta ggagaatgta atgtataaga
     7861 caaaaatact ggaaaatggg cttacaataa ttggtgaaga aataccatat ctaaagtcaa
     7921 taacattagg tatttggata aatgcaggct ctaggataga agaagcacag gtgagtggaa
     7981 tttctcattt tatagagcat atgatgttta aaggaaccaa aaacagaaca tcaaaagaga
     8041 ttgctagttc aatagataat ttgggtggtc aaataaatgc atttacaagt aaagaatgta
     8101 catgttatta tgtaaaacta atagatgagc atatagatac aggaattgat gtattgagtg
     8161 atatgatact aaattctaaa tttgataaaa atgatataga taaggaaaga cttataatat
     8221 tggaagaact taagatgtat gaagactctc cagatgactt atcttatgat ttattagtgg
     8281 aaaatatata tgctaatgat ggattaggaa tgaatataat tgggacaaaa gaatctctgt
     8341 acaatataac tagagaatcc atgttagaat acttaaataa gtattatatt ccaaataacg
     8401 ctgttatttc tatagctgga aactttaact ttgatgatat ggtggagaaa atcaaatcaa
     8461 aatttggaca ctgggaaaag aaaaatttaa gtatagacat aagtgaggct aagtttaatc
     8521 cttgctttat atcaaaaaat aaagatactg aacaggtgaa cttagctatg tgcctaaaag
     8581 gtattccttt tgaaaatgat gaagaagtct attcaatggc agtggtaaat aatattttcg
     8641 gtggaagtat aagctcaagg ctttttcaga aaataaggga agaaaaaggt ttagtatatt
     8701 caatatactc atcacagaca ttatatagga aatgtggtga attaggtata tttgcaagca
     8761 tgagtacaga aaatttacaa gatgtatata atttaataaa gaaagaaatt gaaaatataa
     8821 gagaaaacta tcttacagaa aaagaaatat ctgaaagcaa ggaacagtta aaaggaaatt
     8881 atatattgga tttagaaagt actagcagta gaatgatgtc tactggtaaa tctatgttac
     8941 ttagcaaaaa agtaaaaact acagatgaaa tacttgaatg tataaataat gttaatatta
     9001 attcaataaa gaaggttgtt gataaggtat tcaatatcga aaatattggt acatgtatag
     9061 ttggaagaga tgtagaaaag attctacact tggattaata ttgaaaaaag taaatctctg
     9121 gaggtataaa atatgaattt atctgaaata gcaggtaagg aaatagttaa cttggtcaca
     9181 ggagaaagat tgggggtcat tggagaatgt gaccttatac tagatgaaac tacaggcgat
     9241 atattagcgc ttctaattcc aaaagaaaaa ggattttttg ccagaagaaa agataagtca
     9301 tttttagaag taccatggag aaatgtaaaa aaaataggta atgacatgat aattatagaa
     9361 tatgagggat atatttagga ggaaaaaatg agtattttag tgcaaaagtt tggtgggaca
     9421 tcagtagaat catatgaaaa aatgaatgaa gtatgtaaga tagtaaaagc atacaaaaaa
     9481 aatgatgaag aattacaatt agtattagta gtttcagcaa tgggaagaaa aggagctcca
     9541 tatgctacag atacacttat aaatttgtgt agtgcagtaa atgatgagcc aagtaaaaga
     9601 gaacttgatt taataatgtc ttgtggagaa ataatatcag gaacaattct tgctaattta
     9661 ttgaatgcac aggggataga ttctgttttc tttactggtc agcaagcagg tataattaca
     9721 agtgatgaat attctaatgc aaagataaag tatataaatc ctaaaaaggt aaaaagagct
     9781 ttagatgatg gtaaggttgt agttatagct gggttccagg gtgtaacaga tgatggagaa
     9841 ataactactc ttggaagagg cggaagcgat acatcagcag ttgctattgg taaagcttta
     9901 gaatgtgaaa cagttgaaat ttacactgat gtggatggta taatgactgc agaccctaga
     9961 gtagaaccaa acgctaaagt attgagtttt atagactatg aggaagtatt ccaaatggct
    10021 gacaaaggtg caaaagtaat acatccaaga gcagtacagt tggctaaaag tggaaatata
    10081 actttagcca taaagaatac aatgaatcca acttttgaag gtacaaaaat aggttcttta
    10141 tgtagacact tagaagacaa tatagaatat gaacaagaaa gagattttaa ggttgctgtt
    10201 gcaaataaag atagcgtagc acaagttaag attaagtcag cagaagaagt gtttacagaa
    10261 gtattaaatg agatagaaaa gaaacttata actatagata tgataaattt ctttatatca
    10321 gaaaaagcat ttgttgtaga agatgctgat ataaaatcat taaaggaaat actagataaa
    10381 tttgaattag attatgaagt aaatagagac tgtgctaagg taacattgat atgttcaaga
    10441 attgctgatg aaatgtctgg tatcatgtca aaggtagtta gaggattatc aaaagcaggg
    10501 gtatctcttt tacaaacatc agactcaaat atgactatat cttgtttagt tagtgaagaa
    10561 gatatgcata cagcagtaca tgctatacac caacaatttt atttaaaata aactgtataa
    10621 tacaattatt ttttaatcaa atttaagaat ttcatatata tcttaatata tttgatttat
    10681 ttggtaaaac taaattaaat ataattttga taggagtttt tttatgaaag attatagaga
    10741 agataatgaa aaatttgata aagataattg tagtgaagaa tctagttgcg aagagagaga
    10801 atgtataaag caatttggta ccaatgaaat gccacctcaa cctcctaaag atattcaatg
    10861 tataactata attggagaaa ttgagggaca ttttatagga aatcctcaaa aaaaagctac
    10921 aaagtacgag catataattc caatgctata ctcaattgag gaaagtaatg atgttaaagg
    10981 agtgttagtt gttttaaaca ctgttggagg agatattgaa gcaggtttag ctattgcaga
    11041 acttttaaac agtacatcta aaaaagtagt tactcttgtt ctaggaggaa gtcatagtat
    11101 aggtgttcct cttgctacag caggagatta ctcatttata gcaccaacag caaccatgat
    11161 tatacatcca gtgcgaacta ctggtcttgt gattggtata aatgagactt ttgaatattt
    11221 taaaaagatg caagatagaa taattcaatt tataataaga acatcaaata tcaaaaaaga
    11281 tgtacttgaa aaacttatgc atgaaaaaga tgaacttgta agtgatgttg gtagtgtatt
    11341 aataggaaaa gaagctgtgg attatggtct tatagatgaa gttggaggac taaaagaagc
    11401 acttaaaaaa ttaagagagc ttataaaaga atctgaggaa gaaaaaaata atgattaaaa
    11461 tttgtttaaa taataaaatt tatgatataa tgatgtaata aagtaggagt gttagtgtta
    11521 aggcattagc actttttgct gtgaaggggt aaaacttgct aaaaataagg agtgatatta
    11581 atggccacga aaaagaagaa aaaaaagaag gtaaatacct taagttttaa tgcagaatat
    11641 cataatttaa taacaatatt tataggtgta tttttattat atagtttaaa ttctaattct
    11701 atgggatgga tacctgtgtt aatgcaaaat ctttttaagg gactatttgg agggctttca
    11761 atagcaatac catttatagt aattataaca ggacttttag gattttttga tggaaatgaa
    11821 tatatatata ggctaagaaa gactaaacta tactacataa ttattttatt tatatttgta
    11881 ttttatggat tattaaatgc aggaactctt cctgtagata gcccactaaa aggtaacatg
    11941 tttgatgatg ttatgaaatt gggtgtaagt ggacaaggta gtggtttgat agcaacaact
    12001 attgcgtatt atatgagtaa aatatttggt atagcaggtg gatggttaat aagtatcttc
    12061 gcacttatac tttctgtaat gtttatattt aatatttcta taaaagattt ggtgtcaaat
    12121 gcaaaatcaa aatctaaagc atctaaagac agcaatttaa cgtttaaaga taaaattgca
    12181 aatatgaaaa aatctgcaat agatatgatg actgatgaag tagatgatac tactataaat
    12241 aaaaaaccag gtttctttaa agggttaatg tcaaaaggtc ttaataatga tgatgaagaa
    12301 gatgaatatt ttgaagaaga aaatagtgat ggtgtagatg ataaaactat aaaaatagtt
    12361 ggatttaata aggcagaaga tgagtattta gaaatattag aaggaactca aagtatgcca
    12421 gagttagatg ttttaaaaga attacaaaaa gctaccaatg aaaatccagt tgtagatact
    12481 aaaccagaaa aaaaagttga tatagctaaa cctaatttaa atatagaaaa aactcaacct
    12541 atgagtattg tagcagagcc agttaatgag gattattcaa actataaaaa gcctagcatt
    12601 gagcttttaa ataaagtaaa taagaagtct gatgaaaatg gtaaaaagaa agtgttgaaa
    12661 aatgcttctc ttttagaaaa gacactatca gattttggag tggaagcaaa aattaatcaa
    12721 gttacagttg gacctactat aacaagatat gaaatacaac caagtccagg agtcaaggta
    12781 agtaaaattg taaatttaac tgatgatata gctttaagtt tagcagctaa aagtataaga
    12841 attgaagccc caattcctgg taaaagtgca attggaatag aagttccaaa tgaagaagct
    12901 caaatggttg gtgtgagaga agttctagaa agtgaagaat ttaataattt taactctcct
    12961 cttgcaatgg gacttggaaa agacgtagca ggaaaaatta taattggaga tataggtaag
    13021 atgccacact tactaatagc tggttctaca ggttcaggaa agagtgtgtg tgtaaataca
    13081 ttaataagta gtattttata taaggcaaat cctgatgaag taaaattact attaatagac
    13141 ccaaaagtag ttgaacttgc aaattataat ggaataccac atctattgat accagtagtt
    13201 actgacccta aaaaagctgc aaatgcttta aattgggcag ttacagagat gaatagaagg
    13261 tacaaattat ttgcagatgc tcaagtaaaa gatgtaacaa gttataatga gaaagcggaa
    13321 gaaaaacttc ctaaaatagt aataataata gatgagttag cagatttaat gatggcaagt
    13381 gcaaatgatg tagaagatta tatttgtaga ttagctcaaa tggcaagagc tgctggaatg
    13441 cacttgattg tagcaactca aagaccatct gttgacgtta taacaggggt aataaaggct
    13501 aatataccat ctagaatagc atttgcagta tcatctcaaa ctgattcaag aacaatcctt
    13561 gatatgggtg gtgcagagaa gcttctaggt aaaggtgata tgttattcta tccacttggt
    13621 gcagcaaaac cagttagact tcaaggggca ttcatatcag agagtgagtc ggaaaaagta
    13681 atcgattttg taaaaagtca ggttaaagat ggaataaaat atgaagaaga tataattgaa
    13741 actatatcta aggttaatac ttcaaagggt agtgatgagg atgagttttt gagtgaagcg
    13801 atagaatttg tcgtggaaag tggtcaagca tctgcctcta tgctacagag aagatttaaa
    13861 ataggattca atagagcagc tagacttata gattctatgg aagaaagagg aataattgga
    13921 gcaagtgagg gaagtaaacc gagaaaggta ttaataagca aacaggattt gcagaattta
    13981 gaaggtgaat agaattgtca gttataaaaa tagaaaaagc acttaagcta gataaagtaa
    14041 tatttgtaga tgtgagaact gaaggagaat atgaagaaga ccatatattg aatgcaatca
    14101 atatgcctct ttttaaaaat aatgaacata atgaagttgg aacaatttat aagatgcagg
    14161 gaaaacatga agcaatacaa aaaggatttg actatgtatc gtataaatta aaggatatat
    14221 atttacaagc agcagaactt gcattaaatt atgataatat tgtcatatat tgtgcaagag
    14281 gcggaatgag aagtggcagt atagtaaatt tattatcatc attaggtgta aatgtatatc
    14341 aacttgaagg tggatataag gcttatagaa attttgtttt agagtattta aggcatgtaa
    14401 tggatacaaa aaactttgta tctgttcatg gattgactgg tacaggaaag actgatttgt
    14461 tacatttatt agatgaaaaa ggaatagacg tgctggattt agaaggcata gctaaaaaca
    14521 gtggttctac ctttggtttt ataacttttg ataaaaagcc accatctcaa aagaactttg
    14581 aaacaaaact atttgaaagt atattttttt cgaaagagaa ttatatattt gtcgagagtg
    14641 aaagcaaacg agttggaagt gttacagttc caaatgaaat tttcgaggca atgacacgag
    14701 aaggacatca tatattatta gaatgtgata ttgaaaatag agttgataga ctttgtagag
    14761 attatattta tgataaagat gaaggtaata tattgatttt aaaagaatgt ataaacaagt
    14821 ttagaaaaag attgggtaat aaagtagtag atgattatat tgatttatta gaatcaggtg
    14881 aatataaaga acttgtaaaa agatatcttt tagaatacta cgacccactt tatatgcatt
    14941 cagtagagaa atataagtat agtaagatta taaattttaa tgatacacaa aaagctttag
    15001 aagatgttat agacttatat gagagtatat tggaaggaga aagtgaatgt taaaaatagc
    15061 attggaatca ttaggatgct caaaaaattt agtagatgca gaaataatga tgggaatttt
    15121 aaataacaaa ggctataaat taattggaga ttttgaagaa gctgatgtaa ttatagtaaa
    15181 tacatgtggg tttatagagt cagctaaaca agaatctata gatacgataa taaactttgc
    15241 agagttaaaa aaaactggca atttgaagtt gttgatagta acagggtgtc tagcccaaag
    15301 atattctgaa gaattaaaaa ctgaaattcc tgaaatagat gctatagtag gaacaggaag
    15361 ctatcaaaat atagataaaa tacttaaaga attaagtgaa atgcatcaaa ttgtaagcct
    15421 taatgatata gagtttgtat ttaatgaaga tttaccaaga tatatatcta cacctagcta
    15481 tatggcctat ttaaaaattg gagaaggatg ttccaataat tgtacttatt gtataatacc
    15541 aaaactaaga ggtaaatata gaagtagaaa atttgaagat ataattaaag aagctaagaa
    15601 attagctgaa tctggagtaa aagaacttgt ggttatagca caggatacga caaagtatgg
    15661 ttttgattta tatggaaaag aaagattatc agaactttta gaagaacttg caaaaataga
    15721 tggatttaag tggataaggg ttatgtattc atatccagaa tctataacag aagaattgat
    15781 acaagttata aagaaatatg ataatatatg tagttacttt gatatgccaa tacagcatgc
    15841 aagtaacaat attttaaaat taatgaatag aaaaactact aaagaagata tattaaataa
    15901 aatcaatctg ataagaagta atataccaga tgctatactt agaactacaa taatagtagg
    15961 gtttcctggt gaaacagaag atgacttcaa acaacttgtg gactttgttg aagaggttaa
    16021 atttgataga cttggtgcat ttgcatattc aagagaagaa gatacaccag cagatagatt
    16081 gccaaaccat atagatgaag aagtaaaaat acaaagaaga gatactttaa tgatgattca
    16141 acaaaaaata tctgaagaac ttaatgacaa aaagatagga aaaacttatg aggtgttaat
    16201 agaagaacag atagaagata atgtatatac aggaagaaca caaggtgatg cagaagaaat
    16261 tgacagtata gtgtatgtaa aaagtgtaga caatcttgaa gtaggagaat ttgtaagtgt
    16321 tcaaataaat gatgcaatgg aatacgattt aatgggagat gttttatatg aacttgccta
    16381 acaagttaac tttgtttaga atatttttaa taccagtatt tgttttaatt atgttgctca
    16441 atgtaccaaa taaattttta atagcttgta ttatttttat aatagcttca attactgatg
    16501 ctttagatgg aaaaatagct agaaaataca atctagtcac tgactttgga aaatttatgg
    16561 acccattagc agataagtta ttggttattt cagcacttac atgtatgata gaagaccatc
    16621 ttgtaagtag ttggatggta attataatag tggctagaga gcttacagtt agcatattaa
    16681 gagctatagc tgctgctgat ggaaaagtaa ttgctgctgg aaacagtgga aaacttaaaa
    16741 cgataactca aatggtatct atagtgtttt tattgttagg tgctcagttt gaaaatgtat
    16801 tgatattaaa tattggtgaa attctcattc ttatagctac actacttaca ttatattctg
    16861 gatgggaata tctatataaa aataaagagc tttttatgtc ttctaagtaa aaaattatgc
    16921 tttaataaat aaaaactaat gattaataaa taaaaaataa caacttaaaa ttttaaaaat
    16981 gtaaaaaagg gtatactatt atagtatacc tcttttcatt ttaagaatat tttcttttgt
    17041 gtatataatt attaataatg gtttataaat aaaatgtaat gaataaaaat ataatcatta
    17101 gtagcgtttt taaattgact aggtttgatt tatgttatat aattaatata taacaagaga
    17161 acaaatgttt gtagataaat gaagggatgg tatagagatg agtgtagatc aagaaaaatt
    17221 aaaagcgttg aatgaagctt taggtaaaat tgaaaaagat tttggtaaag gttcagtaat
    17281 gaaattggga gaagcaacat ctatgtctat agatgttata tcaacaggag cgattggttt
    17341 agacatagct attggtatag gaggtctacc tagagggaga atagttgaag tatatggtcc
    17401 agaatcttct ggtaaaacta ctgttgcgct tagttgtgta gcatcagctc aaaaagatgg
    17461 aggaatagct gcatttatag atgcagaaca tgcacttgac ccagtatatg caaaagcttt
    17521 gggtgtggat gttgataacc taataatatc tcaaccagat acaggtgaac aggctttaga
    17581 gatagcagag gcattgataa gaagtggagc gatagatata atagtaatag actcagtagc
    17641 agcattagtt ccaaaggctg aaatagatgg agatatgggt gattctcatg taggtttaca
    17701 agctagactt atgtcacaag cacttagaaa gttaactggt tcaattaaaa aatcaaattg
    17761 tgttgctata tttataaacc agttaagaga gaaagtagga ataatgtttg gaaacccgga
    17821 aactactact ggaggacgtg cactaaaatt ctattcatca gttagattgg atgttagaaa
    17881 aatagataca ataaaacaag gtgataaagt tataggtagt agaactagag ttaaagttgt
    17941 taaaaacaaa gtagcaccac catttaagca agctgaattt gatataatgt atggagaagg
    18001 aatttcaaaa attggagacc ttttagatat agctgctgat gtagatatag ttaaaaaatc
    18061 aggttcatgg tatagttaca atgatactaa acttggacaa ggaagagaaa atgttaaaaa
    18121 attcttggaa gataatttag atttaactac tgaaatagat gagaaagtta gagcatttta
    18181 caatttaaat gaagaacatg aagaatcagg tatttcagta tcaaaagaaa ttgtagaaga
    18241 ataagtaaaa atacaaaaaa ataactagta attaaagaca tgccacgctc cctattagct
    18301 tgacacaatg ttgaaaaaat tataaaatta agtgaggata aaatatctaa atttaatttt
    18361 aattattagg aatatttttt aatcatagtg atgacatttt gggattatta acgaaggagg
    18421 tggatgtcat agatagtgta gtaatgattg taataggtgc agctgttggt attatagctg
    18481 gatattttgt tagaaaaaat atatctgaag caaaaattgg acaagctgaa aacttggcca
    18541 aagaaataat agataaagca caccacgact cagaaacagt acaaaaagaa aaattactag
    18601 aagctaaaga agagatccat aagtggagaa cagaagctga aagagaaaac agagaaagaa
    18661 gaacagaagt acaaaagtat gaaaaaagag tagtacaaaa agaagaagtc ttagatagaa
    18721 aattacaaaa tttagaatct aaagaagtaa acctaagtga aaaattaaag gtagtggaaa
    18781 agaaagaaga agaagttgaa gtaataaaaa ctcaacagtt agaaaaatta gaaagtatat
    18841 caggaattac ttctgataag gccaaagaaa tcatcttaac caatgcggag agagatgtaa
    18901 gacgtgaaat gtctataatg ataaaagaga tagaaagtca agctaaagaa gaagctgata
    18961 agaaatcaag agaaattata ggatatgcaa ttcaaaaatg tgcagcagat cacgtagccg
    19021 aaactactgt aacagtagta agcttgccta atgatgaaat gaaaggtaga ataataggta
    19081 gggaaggtag aaatataagg actttagaaa ccttaacagg tatagacctt ataatagatg
    19141 acactccaga agccgtaatt ttatcaggat ttgatccaat aagaagagaa attgcaagaa
    19201 ttgctctaga aaaattaata gcagatggaa gaatacatcc agctagaatt gaagaaatgg
    19261 ttgaaaaggc gagaaaagaa gttgacaata ttataaaaga atatggtgaa caagctgcat
    19321 ttgaaactag tgtacatggg attcatccgg aattaataag attattaggt agacttaact
    19381 atagaactag ttatggacaa aatgtactta aacattctat agaagttgct catatagctg
    19441 gtataatggc tgctgaaata ggagcagata ttagattggc taagagggca ggtttattac
    19501 atgatatagg taaggctgta gaccatgaaa tggaaggtac acatgttgaa ataggtatgg
    19561 atttacttag acgttataaa gagtctaaag aagttataca tgccatgagt actcatcatg
    19621 gagattacga gcctcaaaca gtagaggctg tattagttac agcagcagat gcaatatcag
    19681 ctgctagacc tggtgcgaga agagaaactt tagaagcata tataaagaga cttgaaaaat
    19741 tagaagaaat agctaattca tatgaaggtg tagaaaaatc atttgctata caagctggta
    19801 gagaaataag aataatggta aaaccagaag ctataaatga tgaagaaata catctattgg
    19861 ctagagatat gactaagaaa atagaggatg aacttgaata tcctggacaa ataaaagtaa
    19921 gcataataag agaaacaaga gctatagagt atgcgaagta aaagaaataa tatctaaata
    19981 ataagaaaat atttttagaa atttaaattt gccacaagat atgataatat atcttgtggc
    20041 ttttaaattg tttaagttta attttatata atgcaaaaat aatctaagtg ctaaaagtgc
    20101 tacttacaaa agattaataa aataaagctt tatatatgta ttatttatat aattatccaa
    20161 gtttgtt
//
LOCUS       DAMJWS010000079        19830 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMJWS010000079 DAMJWS010000000
VERSION     DAMJWS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.080"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            205..1632
                     /locus_tag="PC399_002891"
     CDS             205..1632
                     /locus_tag="PC399_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF2593738.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIGSVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(1709..1978)
                     /locus_tag="PC399_002892"
     CDS             complement(1709..1978)
                     /locus_tag="PC399_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593739.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMRGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(1981..3423)
                     /gene="nhaC"
                     /locus_tag="PC399_002893"
     CDS             complement(1981..3423)
                     /gene="nhaC"
                     /locus_tag="PC399_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2593740.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(3448..4614)
                     /locus_tag="PC399_002894"
     CDS             complement(3448..4614)
                     /locus_tag="PC399_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2593741.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKYYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(4839..6959)
                     /locus_tag="PC399_002895"
     CDS             complement(4839..6959)
                     /locus_tag="PC399_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2593742.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSNNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            7192..7593
                     /locus_tag="PC399_002896"
     CDS             7192..7593
                     /locus_tag="PC399_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593743.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQIGK"
     gene            7636..7929
                     /locus_tag="PC399_002897"
     CDS             7636..7929
                     /locus_tag="PC399_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF2593744.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            7944..8384
                     /locus_tag="PC399_002898"
     CDS             7944..8384
                     /locus_tag="PC399_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF2593745.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(8476..8949)
                     /locus_tag="PC399_002899"
     CDS             complement(8476..8949)
                     /locus_tag="PC399_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2593746.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(8949..9737)
                     /locus_tag="PC399_002900"
     CDS             complement(8949..9737)
                     /locus_tag="PC399_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2593747.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            10065..11309
                     /locus_tag="PC399_002901"
     CDS             10065..11309
                     /locus_tag="PC399_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2593748.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            11632..12729
                     /locus_tag="PC399_002902"
     CDS             11632..12729
                     /locus_tag="PC399_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2593749.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            12720..13418
                     /locus_tag="PC399_002903"
     CDS             12720..13418
                     /locus_tag="PC399_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2593750.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            13421..14485
                     /locus_tag="PC399_002904"
     CDS             13421..14485
                     /locus_tag="PC399_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2593751.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITSMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            14668..15372
                     /locus_tag="PC399_002905"
     CDS             14668..15372
                     /locus_tag="PC399_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593752.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            15365..16687
                     /locus_tag="PC399_002906"
     CDS             15365..16687
                     /locus_tag="PC399_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2593753.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            16874..18247
                     /locus_tag="PC399_002907"
     CDS             16874..18247
                     /locus_tag="PC399_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593754.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEAFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(18619..19557)
                     /gene="pdaA"
                     /locus_tag="PC399_002908"
     CDS             complement(18619..19557)
                     /gene="pdaA"
                     /locus_tag="PC399_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2593755.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         7325 a         2498 c         3092 g         6915 t
ORIGIN      
        1 taaatgatta actaaggaaa tgttgttaca aactttagtt atataaaaaa ttcatttgat
       61 gagcaaagta ctaatatcaa gtaagaataa aagcttatta gataagaaaa ataatatttt
      121 ataataatga tgttacttaa attattatgt tatactattt taaataggaa aaatttagat
      181 actataattt tagggggagt caagatgaaa ttaactaaat caatatcatc aatgctgtgc
      241 ttatctctag ttttaagctt atcaccagta gcatatgcaa atgaaataag tagaacaaca
      301 gttgagaata ctttagtagg tcaggataga tatcaaactg ctgttgaagt aagtaaaaaa
      361 ggatggagta gtgcaaatga ggctgtagtt gtaaatagtg aatctattgt agatgcatta
      421 gctgtaacac catttgctaa attaaaaaat gctcctatat tattgacaga aaaaaataat
      481 ctaaattcac aaactgagaa agaattaaaa agattaggag ttaaaaaagt ttatataatt
      541 ggtctatcta gttctgtatc aaatgatgtg caaaaacagt tagaatctaa taatttaact
      601 actgatagag ttggtggtag tgatagatac caaacagctt tgagtatagc taaaaaaata
      661 gaaggattaa aagatatatc agaaatagct gtagtaaatg ggtacacagg tcttgcagat
      721 gcagttagta taggttctgt agctgctact aatggaatgg ttatacttcc agtatctgat
      781 gcatctggta tatctagctt taaagatttt atagaatcta agaatataaa taaatcatac
      841 ataataggct ctactaatgc tgtatcagat aaaataaaac aaagtttacc taattcagaa
      901 agaataggtg gagcagatag aaatgaaact aatggtaaag taatagaaaa gttttataca
      961 tctaataaat taaacaatgt atttgtggct aaggatggaa tgaaaaatca aaatcaactt
     1021 atagacgctc tagcagtagg agtactagca tcaaaagaaa attctcctgt actaatcgta
     1081 ggagaacaat taagtgcaac acaaaagagt gtattttcga ataaaaatgc tacctctata
     1141 actcaagttg gtagtggagg aaatgaaaat gcatttaaag aattaaaaga tttacaggca
     1201 aatagtaaca ataagagagt aatgtatgta actaatacag ataaggtaaa tataagaaat
     1261 gatgctacaa tagaagcttc tgttatagga tatctaaata atggtgatga agtagaagtt
     1321 ttagatgtgt taaaaactgg atgggtaaaa ataaaataca atgaaggtat tggatatgtt
     1381 agtgggtctt atttgacaaa taataagcct gataatagta atgaaaatat aaaaataaag
     1441 tatgtaaaag aaaaagatgg tctaaatgtt aggaaaggac caagtacaga agatgaaaaa
     1501 ataggtcacc tttcatatgg aagcaaagta gaaacaatag aaatgtttgc tactggatgg
     1561 gtaaaaataa agtacaatgg tggatatggt tatgtaagta atgattactt atctgataca
     1621 ccagttattt aattataagt aacaataaaa aaactgcttt aatttgtaaa gcagtttttt
     1681 tataaaaatt taagtctaat aactatttct attttgatac cttaatcctg ggatttatta
     1741 ttatggatat tatcaacata caaacactcc aaatccagtg tctaattcca aacttagata
     1801 ttaataaatt cagtcctgct atggtaaata agcagtattt aattacaatt aaaagtgtat
     1861 ttatagaaag ccccctcata gaaagtatcc taaatccaat aatagacatt ctaagtccta
     1921 taaatgccaa taatatacct gcaatatcct gaattaagta atagtattca aatttcatat
     1981 tcaatcaact cttctctgag cagtatcaaa tatttctaac tcttcaattt catcatctgt
     2041 gtatgtaagc atagtaaaac cagttacacc ataaataaat gaaattatag gtgtaatcca
     2101 acttaagaaa caatatggaa taaattctag tggacttaca ccaagagtct gagcacagaa
     2161 aacagcattt gaattccatg gtattaatgg accaccaagt gtagctgtat cttctataat
     2221 tctagataga ttttgtggtt ttaatttaaa ttctttgtat attggagaca ttagagttcc
     2281 gacaaataca gaagtgaaca tcattgatga actaaatgca tttgcaaagt agcttactat
     2341 aaaagtagac ccaaccaatt tagttcttga ttttcctatt ttagttataa gaggttgaag
     2401 cagtacttct aagaatccag ttttttggag aataccagca ataacgaaaa ctacaaatac
     2461 tagtaaaacg gtttcagcca tactcattat tccaccacga tttaatagtt tgtcggcata
     2521 gacaaatcca gtatctatag taaatcctga tagcatgtaa tttaaaacat cacctatttt
     2581 ttctccttct tgaaatactg ctataataag tcccatgata gctgaactta gtatagatat
     2641 aatgggtggc ttttgtagca ataaaagtaa taataaaaat attattggaa tcattgctac
     2701 taaaccaata tgaaagtttg aatttaatac atccttgatt tgatttattt gaacatagtc
     2761 tatagtatta tttgaatact taaatccaat tactgtatac agaactatac atattacgta
     2821 tgcaggtcct gtggtataaa acatatgctt catatgtgta attacatctg tcttacaaac
     2881 tgctgcagct aagtttgtag aatctgatag aggtgacatc ttatctccaa agaaagcacc
     2941 agaaatgact gcaccagctg ttaaaccaag aggaataccc atactagtac caatactcat
     3001 cattgctata ccagaagttc caagagtccc ccaagatgta ccagtagcaa cagaagttaa
     3061 tgaacatacg attaatgttg taagcagaaa atatttaggg gttattattg ttagtccact
     3121 atatactact gttggtattg taccagcagc tatccaagct gctattagta caccaacaga
     3181 aagtataatt aagtttgatt gaaaggcatc tttgccaact tcaaatccaa aagcttctat
     3241 ttcatcatta gtataaccaa gtttcatggc tgcaggaact actataagcc atgaaagtaa
     3301 aaacattgta gttatagaag cattaaatac aactatacca actgatgtta gagctattat
     3361 tgcaagcatt actaacaagg aatatctaaa tgaaggtatt tttctttctg tattcatttt
     3421 catagtgcaa attctcctta aatattttta gttaattgca ttttttattc ttataagagc
     3481 ttcttcaagc atacttcttg gacaagctaa gtttattctt tgatacccac ttcctccaat
     3541 gccaaagctg tttccttgat ttagagcaac tttacctttt tgtacaagta tactttctag
     3601 ttcctcatca cttaatccaa gtgcactaaa gtctacccat aataaataag taccttctgg
     3661 ttttctaact ttaagttttg gcatattttc atttatatat tttatagcaa aatctatatt
     3721 agattctaaa tattctaaaa atgattctag ccaagattca ccattattat atgaagcttc
     3781 agttgcaact aagctaaagc aattatttct ctttatatca attctggtaa aggcatcatc
     3841 taacaattta tagtattttt catcaggtag tattacatat gatgattgaa gaccagctat
     3901 attaaaagtt ttagttggag ccatacaagt aattgtattt ttttcaaatt ctttagaaat
     3961 agatgccata ggaatatgct tatgtttttt taatataatg tcactatgta tttcatcaga
     4021 tatgattttt acattatgct taagacaaat atctcctagt ttttttaatt catcttttgt
     4081 ccatactcta ccaactggat tatgaggatt acaaagtatg aataatttta catctttaat
     4141 cttgttttca atgtcttcat aatccattat gtagtttcca ttttctaatt tttgtaatgg
     4201 gcttataatt agttctctat tgttattttt tacaacacta ttaaaagggc tgtaaactgg
     4261 ttcttgtatc attattttat catttgcttt tgtgagttca tttattaaaa gacttatagc
     4321 tgggataaca cctggactat aaattaacca ctcacttttt attttccagt tatgtctcct
     4381 atctagccag tttactatag attcattgta agaatctggt cttgttgtgt agccataaat
     4441 ctcttgttct agtctatttt ttaaagaatc aattatacaa ggtgcagcct taaaatccat
     4501 gtcagccacc cacataggaa gtaaatcatt agttccatac tttttttcca tttctgacca
     4561 tttagatgaa aaattattgc ttctatcaac aatctcatta aaattataat tcattgtaaa
     4621 tacctccagt aattttaatt ttatgttaat aatattaagc aaacaacgtg ccaatattga
     4681 taataaaaaa tatttattca caagttaata tttaatatta agtaatttca gagcatagat
     4741 aagataaaaa tattaattat acttaaaact ttgtggaaaa ggttatttct aataaatttt
     4801 taaatatatg aaaatgtaat taaataggtt aaataggttt aactgtttaa cttgttagac
     4861 ctatttatat tttttagaaa ctctactccc tttgatgtta taattgaacc tccacgacca
     4921 acaagtatgt ctacaagccc aaagtcttgt agttctaaaa gcatatttct aatctgttgt
     4981 tctgtcaaaa atctattttc ctctaaagct attttacata agctttttct acctattctt
     5041 tcttttaaat cataagcctt ttttaaatta tccaatataa atatgtattc atctagtgac
     5101 cttttaaaat tatagtcgta tttcatgatg tttttgtctt tgccaatgtt gaattgatac
     5161 ttttttatat tattacttac tttattacaa acggttctac tataatttga atctatagta
     5221 tctaatatat attcaggtaa atcacatatt tctataatat ctttttccaa gtaacagaaa
     5281 tattctccta agtttcttaa ttctcttaca tttccttccc atctatacat tttaaagatt
     5341 tcttttactt cctcacttag aataaaatta catggaatat catattttaa tgaaccaaaa
     5401 atttcaaata tatcagcagg tctttcccta agtggatgta ttttaagggg aagaacattc
     5461 aacctaaaat ataaatctct tctaaacata tttttagata caagttcttt taaatttcta
     5521 ttagtagctg caattattct tacatcaatg tcaatgacat tattagaacc aatacgtcgt
     5581 acttgttttt cttgtatgac tctaagtaat ttagattgag aatttaaatc catttctcca
     5641 atttcgtcta aaaatatagt tccattgttg gctaattcaa ataaaccaat tttcccacct
     5701 tttttagcac cagtaaaagc cccttcatca tagccaaata attcactttg cagtagattt
     5761 tcttgaaaag tactacaatt tactgcgaca aaaggaccat cttttctcct agaagcacta
     5821 tgaatggatt gagcaaataa ttctttacca gtaccacttt caccaattat aaggatagat
     5881 gaatttgatt gagccatttt attagcaatt ttttttgtat taatagtttg tatactagaa
     5941 cctaatatat catcaaatgt gtatttactg atattaccag agtttactag ttgtgctctt
     6001 aatttagctt gctttttttc taattgagaa aactttgtca tctttagtat aaatccatca
     6061 aatacattta attgtatatg tttaatctct aaatttatat caatatgatt tattttaatc
     6121 aatttctcaa aaaatggagt tttattggtc aatatgcttt gaaaattgat gtcttttatg
     6181 caatcaccaa caaatgagtc aatcatttca tttgcattta tagacaaaat ttttctagcc
     6241 atatgattat agaattgaat tattccatcg ttatttgtgc atataattcc atcatcaatt
     6301 atagatagta acaaatcaaa ttgtctttca aacttatttg catctaacaa taactcacct
     6361 gtactataac ttgtaggaat tattttttct acgtattttt ttattaggtc atcttttatt
     6421 agatgtttta atttggtttt tgtagcaatt tcaacaatac aacttaaatc tattattctg
     6481 tattttaaat ctataacttc acaattttta aacttaggca agattttttc gccaggagtt
     6541 aggattacag catctgtggg agtaaactca atctctggat agcagggcag gatatttata
     6601 ttgtctattc ctaatctatg tattaaagaa attgtttcta ttgccatatc tttacttaag
     6661 ttatatacat aaataatttt atctttttct aatttggata tcatatcaat actgttcttt
     6721 tcaaatgtca acttaggaac aatgatttga gcatcatagt gagataattt tactatttca
     6781 tcatatttta aataagcaga taggagtata agtttttctt taaaaaactt taaatttcct
     6841 tcttcaaatg agtataaatt tatctttaga ttatcaccta agaattgtct tatttgattt
     6901 tcataaattt ctccagcgtc tttttttaaa cttacaatag ctaattccat ttgtttcatt
     6961 ttaacctctc atacaaattt gacttatttt agattacata ataattaaat tagattaaat
     7021 aatattattt aattattatt ataaatttat tatagactac acattgaagt aaaagaagta
     7081 gctaaaatta ttccttttat ttttgttttt tgtatctatt tggtataata gtattagagc
     7141 gtataataat ttaggcaaaa ttttttaagg aagactagag gagaatataa aatgaaaaaa
     7201 tgtctttact gtaataggga tataaagtca ccaaataata aattagctat aaaatatgat
     7261 gacaatatga atatatatcc atgtcgtgca gaatgtaagg aaaaaattga agaatatata
     7321 gcaaaaaaac ctacatataa gtttataaat atattagagg ttcttttagg tgtgggcgga
     7381 atattctgct ttttgagtaa atggactatt gcagcccaag ttgtacttgg aattgatgca
     7441 ctggttatat ttttattccc attggcagga tttaagatga atggaaagtt aagtatggaa
     7501 caaactaaga atcaaagtag gtctattgca atatcaattt tggcatttat agtaataata
     7561 actgtattat attttaaaca aattggtaag tagagcataa ttgtataaga catcattaga
     7621 gtatgaggtg agtttatgga agaaagaaaa ttagcaccaa acaatgagag ggaagctctc
     7681 ataaaaagat taaaaagaat agaaggtcaa gttaagggta tacaaaagat ggtggaacaa
     7741 gaacgttatt gtgttgatat attggttcaa atatctgcta taaggtctgc tataaataga
     7801 gtaggcacaa ttatcttaga gaatcatata aaaggatgtg tagcagaaag cattaaacat
     7861 gatgatgctg ataaaactga agagatgata aaagagctta tggatactat aaataaattt
     7921 acaaaataag gaggcggtta gttatgaatc aacaaggatt tattatggaa attgttgatg
     7981 atagaactgc aaagttaaaa atgcaaagac attcagcttg tgctgcttgt ggaaaatgtg
     8041 ctactacaaa ttcagcagag tcaaaagaaa tattagtaga agtagacaac acaattggcg
     8101 caaaagttgg tgaccatgta gaagtgagta tggataatat gaatgtatta aaagcagctg
     8161 taatggctta tataatacct cttatagcac ttcttgtagg aacaataggg acttattata
     8221 tattgggggc aattggaatg acaactggag ttgaagccat aagtggatta gttggaattt
     8281 gtcttacaat aatctgttat ttatacttaa agaagaatga caaaaaattt agagacagta
     8341 gagaatttat accagtaata acaagaatat taattgacct ttaagataat tataattaaa
     8401 aaatagcaat gtgcttctta aataaatact caatacgtat ttgtagagtt taagtacatt
     8461 gctattttta tatttttatt ttgattttct gtaaggaata aagttttttc tgattttcaa
     8521 aagattcaat gttcttacaa aagaagttac aactaataca ccaagtgcta ttgcagcact
     8581 tagaaatagc gcatctgtac cttttgacat agccaaagaa taatttttct gaatcaaaga
     8641 caacattgta ttatatagcc caattcctgg tattagagga agaatacctg gaattacgaa
     8701 cacaatagct ggttgcttta gttttcttgc taaagtctca ctgcacgctg agaccaaagc
     8761 agcagcgata aaacctgcaa aaactgcatt tgtagtaaaa ttaaataaat aaacatatac
     8821 aatccaacca attccgccag ttactccagc tggaagaagt agatatagtg gagcattaaa
     8881 gaaaacagca aatccagcag ttgcaacacc tgaaaataaa aaatgcaaaa tcatagacat
     8941 atcagtcatt agaaaggacc tcctattcta taccatgtat caaggacaaa accaactcca
     9001 gaagcaattg caataataat catagcagca tcaaaagcac gagaaattcc tgatattaaa
     9061 ttccctgata ttaaatctct aatagctttt ataaaagcaa ctcctgggag tagtggcatt
     9121 atagcgccga ctataagcat tttagggtca tctaaaatgc ctatttcaac tagtagaact
     9181 cctgaaagtg caattaaaaa agaagatact aaacaggaaa atgcagatat tgcacttatc
     9241 ttcatcgttt tatcataagt aattgcagca aagattgcta ttataaacgt aagtataaaa
     9301 tttaatacat aattccctcc aagcaaacaa gcaaaacaag cagatgcaat tcctgtacaa
     9361 gagtaaaata accataaagg ataaggtttt acatcttgga tttcataaag acgtgcaatg
     9421 gcattatcaa tatcatactc tttgtcacta acaaattctc gagaaaaact gtttaataaa
     9481 gaaatcttat ttaagtttat acctcttgat tgaatcgttt tcataaacga aaagccatca
     9541 aatttctcat ctgaaataat tattactgta ggagttgtaa aaatatttac atgataaaac
     9601 cctcttgacc tgcatattct aaggacactg tcttcaaccc tagatgtttc agctccattt
     9661 tttatcatca gttcccctac aaatagagct agttttagaa tatttttctt atattcaagt
     9721 tcctcatttt gattcataaa tacacctcca aattaaaaaa cgtaatattg aaattatatc
     9781 aaatttttaa gtaatattcc ttaatatcta tacaaatata ttatcttttt tttgtttcaa
     9841 aatagtcaca tttaaattat atccaattaa aagaattaga caatattatt tattatattt
     9901 taacttaaaa ataaagaaag taactatgtt aaatatggaa aaatggcaaa acacttgact
     9961 attaattggt aaaggactat aataataaat tatcatattg ataaaaacga aaattatgaa
    10021 atgtctgtaa aaataaaatt taaataataa ctaggaggag taatatgttt gagaacttga
    10081 aaaaagcgga ccctgagatt tatgaaagta tgaaaagaga gttaaaaaga caacaaagaa
    10141 atattgaatt aatagcatct gaaaacatag tatcagttcc agtaatggaa actatgggaa
    10201 gtcatcttac aaataagtat gcagaaggtt actcaggcaa aagatattat ggtggatgtg
    10261 aatacataga tgaagttgaa actcttgcaa tagatagaat caaaaaaata tttggagcag
    10321 aacatgctaa tgttcaacct cattctggag caaatgcaaa tattggtgta tattttgcta
    10381 tgttaaaacc aggtgatgtt gttatgggaa tgaatttatc tcaaggtgga catttaactc
    10441 atggtgctcc agttaatatt tctggtcaat attataaatt ttatgaatat ggtatagata
    10501 aagacagtgg acttatagat tttgatgaag ttagaaagat agcacatgaa gtaaaaccta
    10561 aaatgatagt tgcaggagca agtgcatatc ctagagaaat agattttaaa aagtttagag
    10621 agatagctga tgaggttgga gcattgctta tggtagatat ggctcatata gcaggtcttg
    10681 tggctgctgg tcttcaccaa aatccatgtg aagtggctga ttttgttaca acaacaactc
    10741 acaaaacttt aagaggacct cgtgggggtg ttattctttg taaagaaaaa tatgctaaag
    10801 acatagacaa agcaatattc cctggtattc aaggtggacc tcttgaacat ataattgctt
    10861 ctaaggcagt ttgctttaaa gaagctttaa gtgatgagtt taaagaatat caggtacaag
    10921 ttgctaaaaa tgcaaaagcc ttagctgaag aacttattaa aagagatttt aaattgattt
    10981 ctggaggtac agataatcat ttaatactat tagatttgac taataaaaat ataactggta
    11041 aagcagcaga aaagagattg gatgatgctt atataacagc taataaaaat acaattccat
    11101 ttgacccaaa tggtgctctt gttacaagtg gaataagact tggtactcca gctgtaacta
    11161 ctagaggtat gaaagaagaa gatatggcaa taatcgctga ggctatagac ctatgtttaa
    11221 cttatgatga agaatctaag gctagaacat tggttgttgg tttaactgaa aaatatcctt
    11281 tatatgaaga atataatatt atggattaat tataaagaac aactaaataa aatacattta
    11341 aataattaca atgattgtgt atgtatttga atatatttag atataaaaaa ggtgtctcaa
    11401 aatgaacttt ttagtttatg aggcactttt ttgtatggaa tcgaacatat ttgtattatt
    11461 taaataataa aaaatatggt tatctctatt tttagatggt ctctttgtat tttttaatta
    11521 aaactttaag atttcttaag aattatacta ggtttttcta aagatttaaa gtctatactt
    11581 ataagtatag taatcatatg aaaagaaaag ctttaacgag gagggaatct tatgaaggta
    11641 ttaattctta caggaaaatt tggtatgggt cattactcag catcaaattc tttaagtgaa
    11701 gatataaaag caaaatttga taattcagaa ataataatta aagatatatt tgagtacatt
    11761 atgcctaact attcagataa aatgtacaag actttttcta ttcttgtaaa tcggggaagt
    11821 agtctttata atttgtttta taaatgtgct gaaaatggaa agaaagatat aaaatttact
    11881 ttttcagatt attttttaaa taaattagat acattacttc atgaagtaca accaacagta
    11941 gttatttcaa cttttccatt ttgttctcaa ttagtatcaa gatataagga aaaatataac
    12001 tcaaatcttc ctttaataac atgtataact gatataagta gtcactcaga atggattagt
    12061 aaaaatacag actgttattt agtagctagt aaatctacta aggaagaact tgtttttaaa
    12121 ggaatagatg aatctaagat taaagttaat ggtattccag taaaaaaaga atttaaaaga
    12181 atagaacatg taaatcattc aactaaaaaa aatattctta taatgggtgg aggattagga
    12241 ttactgccta aatcagaaca attttacaaa gaattaaata gtttagaagg agtaaaaact
    12301 actgtaataa ctggtaataa taaaaaaatg tactataaac tatatggtag atatgaaaat
    12361 atagaggtag taggttacac caatgaagta tataaatata tgaaagattc tgaccttatt
    12421 atttctaagc caggtggaat aacactattt gaaacaattt attcagaact tccaatattg
    12481 gcatttaatc catttttaca acaagagata gataatgcta gttttatatt aaataatgaa
    12541 attgggagaa tacttggtaa aaataaaaaa tactatgtag atgagataaa agatttaatt
    12601 tatgatgatg ctactcttaa agaaatgagc agtaacatga aagaattaaa aaaacaattt
    12661 gataataata ccttagagaa tattttattt tcacttgatg aacaaggagc gtgtagagaa
    12721 tgtatgtagt aggtttaatt atcgttgttg cattgatttt tttagtccat tcaattatac
    12781 ctacatacta taataaacta ttaaataaag aggtattaaa aaatatggct ggagaaaatg
    12841 aaattgcact tacatttgat gatggtccag ataagagata tacagaaaaa ttactagatg
    12901 tgttaaaaga aaatgatata caagctatgt tttttgtagt agcaaagaat gctgaaaaag
    12961 agcctgaaat tataaaaaga atgttgagag aaaatcatat agttggttta cattcattgg
    13021 aacacagaaa tgcatggctt tatagttata gttatgttaa aaaagatttt attgaaagta
    13081 caaatataat gaaaaattta ggtgtagatg taaactacta tcgtccacca tggggtcata
    13141 ctaatatatt ttcaaattca tttgttaaaa aatataatct aaaaatgact ttgtgggatg
    13201 taatggcaga agattgggag aaagatagta cagtagacat tattataaac aagttaatga
    13261 gtagaacaaa agaaaattct ataatttgtt tacatgatgc aggtgaaaat tcaggtggtg
    13321 cagtaggtgc acctgagcgt actattgaag ctttaaagat agctattcca aaacttaaag
    13381 ccagtggttt aaagttcgta acaccagaaa ggatgtagtt atgataagta aaactgaaaa
    13441 gaaaaataaa tgcctaggta gtgtagcttt tttagtttta ctaatgggca taacaggata
    13501 ttttgttttt agagggcagt cagttgaatc attgataaaa tcattaaaag gtgcaagccc
    13561 tatgtttata ctaattggat ttgcaatgat gtttatatat gttgcctgtg aaggtataaa
    13621 tatatactta ggtatgaaag ctttaaacca aaagactact cttttaaaat gtatgggata
    13681 tgcttttatt ggattttatt ttagttctat tacgccttca gcatcaggtg gtcaaccagc
    13741 acaagtttat tatatgaaaa aagacgatat aaatatatca tattcttcac ttatactatt
    13801 agtcattgtt gttattcacc aagtagttat actggcatat agtggaataa tgttcattat
    13861 ggaacgtgag tttatattaa ataatgtaag tggtatgaac attttactaa tatatggtgt
    13921 aataacaaat gttgctcttg tcattggagt aattgcaatt atattttcaa aaaaacttgt
    13981 aaacaatttt ataatatcaa taacaaattt attaggtaag ttaagaataa taaaagatgt
    14041 tgaaagttca agaaaagtta taatatctca aattgaagag tatgttaaag gtgctcaata
    14101 tataaagcag aatccaaaac ttgtagtcca aattttagtc ataactattg tacaaataac
    14161 atctatgttt ttagttcctt tctttgtata caaagctttt catctatcaa catatactgt
    14221 atttgagata ttagcgatac aatccttgct taatattgct gtttcttcat taccattgcc
    14281 aggggctgtt ggagcttctg aaaatagttt tatgacatta tttaagatat ttttccctgg
    14341 tcacttatta gttccggcaa tgttacttag taggggaata agtttttatg catttgtagc
    14401 tataagtgga ttgatatgta tagtagtaca tgtaaaatct tctgaaaaag taaaagcagt
    14461 gaaaaaagtt gcttatgtaa gataatatta tatagattat ttttattaat atgtcaattt
    14521 gtgtgtatat tttaattaag ctttaaacat gtttacagga gtagaaacta ttaaaacgtg
    14581 tgacattgct aagcttttgc taataaagta taatatttta tatagtttga aatatttaag
    14641 aaatatagca aaaaaaggag cttggtgatg aataaagaga ttccaaaaaa aattttagta
    14701 gctgatgata atcctgagat tagggaaatc gtagaaatac ttttaactgg tgaaggttat
    14761 gaagttatta tggcaacgaa tggagaagaa gctgtaaact tagtagatag tacgattgat
    14821 ttgattattt tggatgttgt aatgcctgta aaaacaggat ttgttgcatg taatgaaatt
    14881 agagaaaaaa cgacagcacc aatattattt ttaactgcaa aaactcaaga ctctgataaa
    14941 gttattggat tttcagcagg aggagatgac tatttatcaa agcctttttc atattctgaa
    15001 ctaatttcaa gggttaagtc gctacttaga cgttattata tataccaggg aaaggaaaag
    15061 gaaagagagc caaatatatt aaaaacaaaa gagttatgtg tcaatttaga tactcaagat
    15121 gttacactaa atggagaacc tgtgtttttg acaactatag aatatagtat attaattctt
    15181 atgttgaaaa atagaaaaaa agttttttcg tctgaaaatt tatatgaaac tatttgggat
    15241 gagcagtatt tttatacagc aaataataca ataatggttc atattagaaa tttaagaaaa
    15301 aagttagaaa aagaccaaaa aaatccacaa tatataaaaa ccgcatgggg aaaggggtat
    15361 tatattgatt aacaaaatta agccatctaa gttgggagtt atactattga tatatgtagt
    15421 aatctcagct gcagtggctt tcattttaaa tataccattt tactctgctg ttgaatctat
    15481 agtaaaagat gtatcagaag ggaaagtata ttttaaaaga gaagtaaatc aacttatgga
    15541 taattttcaa acttatgtga ataacaatgg tataagagca gatgatggag atgatatagc
    15601 aaaatggaat agtgataatt ggtttgtttc tatgaagata tataaaggaa ataaggtttt
    15661 ttatgataca ttatactatc ctaatgaagt acctgataag agtgaggaaa atgagtttta
    15721 tagcagtccc atggctagaa actatgagat acaattttct gatattaaag ctaatatagt
    15781 tgtagtagct ttatttagat ttagatttca aaattttata gaagcaattt ctattacatt
    15841 tatggcactt acatttatta taacattttt actgttgttt agtagaaaga taaagtacct
    15901 aaggaaaata gaaatgggta taaagatagt agagagtggc agtttaaagt atagaatccc
    15961 agtaaaaggt aatgatgaat tatcatcact tgcatctagt attaatgaga tgagtcatac
    16021 attggagaat caacttataa aagaaaggga gatgaagaat aaaaataaag atatagtaac
    16081 ttcgatatca catgatgtta gaactccatt aacgtcagta atttgttatt tagatttgat
    16141 taaagaccat aaatacaata gtcctgagca attggaacaa tacataaaaa atgtaaggtc
    16201 taaagcatat caaataaaag atttaacaga tgaccttttt acgcattttg ttaattctgg
    16261 agaggaagtt tcaaaagggt atgaaaaaat aaatgctaat gaatttattt ttcaattact
    16321 tttagatagt tcatatacat tggaggagaa aggatttcat ataacaatag aaaatgaact
    16381 ttataaccaa tatcatattt atgtagatgt tacacaattt agaaggatat ttgataattt
    16441 atgttctaat ataataaaat atgctaggaa agataagcct atagtatttt taattaaagt
    16501 agaggattct aaattactaa ttatacaaaa aaatagagta agtaattttt ctaagaatgt
    16561 agaaagtttt ggaattggta taaagaattg tgagaaaata gttgcatatc ataatggaag
    16621 cataaatgta aattcagatg ataaagaatt tgctattgaa atatctattc cagcaaaaga
    16681 atcataaaaa tttaaattat atattgtgta attgctatta tatattgtgt aattgcttat
    16741 gtaaaattct atgaagcagg aagcatgtga ctttagttat gggatgttca atattgtgta
    16801 cctctaggca agtaacaagt ttacttacaa gaccatataa tattattatc ttaaaaaaag
    16861 gggacataat aatatggttt tgacaggagc acattatatt tacattctat ttatggtaat
    16921 tatattaatt actatgatta tgaaaaagga tacaatagta ccatgtatat taggtgtttt
    16981 ttttatggga ttgttctttg aaaaaaacat ctttggtgct ataacagcag tgtttaactc
    17041 ttttattata tctttaaatg agttgggtcc aattatcttg attatagcta ttatggttgc
    17101 attatctaaa gccctagaag ccaataatgc tatacagtat atggtgagac cattttcgag
    17161 agttataaaa aattctaata cagcattttt tgtaactgga tttgttatgt tagttctttc
    17221 ttggtttttc tggccaactc cagctgttgc cttagttgga gctgtatttt tacctgttgc
    17281 tatgagagct gggctaccag caattggtgt tgcagtagca ttaaatttat ttggtcatgg
    17341 tcttgcctta tctacagact ttgttattca aggagctcca agtataacag ctggtgcagc
    17401 aggtgttgca gtatcatatg ttataaatga tggtatgatt ttgttttggg tgatgggaat
    17461 cgtaaccata tctatggcat tttatacatt aaaaagagat attaacaaag gaatgtttag
    17521 agaagaacta aaaggttttg aaagtgaaga agtagaagcg ttcaatggaa aatctaaaat
    17581 agcaactatt ttagtagctt tgggatttct tgctgatata attgctatgt atgtatttga
    17641 cctaaaagga ggagatgcat cagctctttt gggtggaaca gcagtatttt taataataat
    17701 tataaatgtt ataaattttg gcaaagatag tcttgaaaat gtatgtgaga atataattga
    17761 tggttttgtg tttgggataa aaatatttgg agcaattata ccaatagcag catttttcta
    17821 tatgggtgaa gttgcaccat tgacaggtgt atttggaaaa gtattagcac caggttcaca
    17881 aggtttatta tcagatatag gcatagctct ttctcaaaca ataccactta ataaatttgc
    17941 cgtatcaggt atagaaactg tagtgggagc aataactggg cttgatggtt ctggattttc
    18001 tgggatttca ttagttggtt cacttgcctc tgtatttgga actgctataa atgcaagtgt
    18061 tggtgcatta gctgcattag gacaaatcag tggtatatgg gtaggtggag gctgtctagt
    18121 tccttggggt cttatttcag cagcggcaat ttgtggggtt agtccaatag aattggcaaa
    18181 gcgaaacttt gtcccagtta taaccggatt ggttgtaaca accatagtag caatgtttat
    18241 tatttaataa atcgaataaa tctttaattg tataaattaa aaatagatgt gtagctataa
    18301 tttattataa ttgttttact aattctaagc catacatcta tttttattga tatttttatt
    18361 agtattcttt aataaagtaa tatttttcat tatattatac ataacatcta aatataacat
    18421 ctaaaatact gttatctata tttaatatct tgccaaattt tttgatgttt tattgccctg
    18481 tcccaccaac atatataagc tatcacaagt ttttacctat actatcacca aacaaaaatt
    18541 ctaattttaa tttattattt tataacctat attatattaa ctatattagt ttgtagactt
    18601 tagatgtaat tataaatatt aatactccaa gagttcaaat tcatatccta gttttcttgc
    18661 atttgatata aaatcattta aaatttctgt actagttttg gaaactgcat gtagtaacaa
    18721 gatagaacca tcatgtagat tatccattat tttttgcgtt gcttcttcat gtgatggttg
    18781 tttatcagta tcccagtctc tgtatgcaaa actccaaaat acagtcttgt atcctaaatt
    18841 tttagtcatt gccaagcttt tttctgagta ttttcccata ggaggtctaa aaaatttaac
    18901 catatctttc ccagttacat ctttatataa cttttcaaca tcatataatt cgtcattaaa
    18961 gttttttaga ttgcttgttt ttgttggcat agatggatgg gttttactat gatttccaac
    19021 tatatgtccc tcagaaacca ttctttttac taaatcttta ttttctttta tatatggagc
    19081 tgtaacaaaa aaaactgctt taacctggtt ttgttttaga gtgtcgagta tttttgtagt
    19141 gtatccattt tcataacctt catcaaatgt aagatatagt gttttttgac catcttttgt
    19201 aggtccattg tatagggcat catagtcaga aaatttaaat tcaatgtccg tatttacttc
    19261 tggtgtttta tgcttattgt ttggtatata aaaccaatca agagtagtgt tatctaaaga
    19321 agatacatct aatttattat tatcattatt agtagtggct tttgatggtt tatctgttcc
    19381 gtctgaaact attacattgg aagtatcttt accactatct attttttcct tatcttcttg
    19441 taactgtgtt tccttatttt gattttcgtt ttgattgttt tgtgaatttg aacatccaac
    19501 aataaaaaaa atagaaatag aaagtagtgt taaataatgc aatacttttc taaacatttt
    19561 gaagctcctt tctaaattaa taataattgc atataataat aattatatga ttaattacaa
    19621 ttgcatatag tattattata attggaaatt actattaata atagttatat ctttaaaaat
    19681 aaagtcacaa tagtatgaca aagggtgaat aatcaataaa tttaaaaaaa aatgaaaaaa
    19741 atagttgacc atattatgag aagatgttat tataataaat gtgctcaaca agaagtaaca
    19801 aaataacaag cacaaagaag ttaattgaat
//
LOCUS       DAMJWS010000080        19123 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMJWS010000080 DAMJWS010000000
VERSION     DAMJWS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19123
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.081"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            156..656
                     /locus_tag="PC399_002909"
     CDS             156..656
                     /locus_tag="PC399_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2593756.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(906..1685)
                     /locus_tag="PC399_002910"
     CDS             complement(906..1685)
                     /locus_tag="PC399_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593757.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1682..2161)
                     /locus_tag="PC399_002911"
     CDS             complement(1682..2161)
                     /locus_tag="PC399_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2593758.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            2660..2950
                     /locus_tag="PC399_002912"
                     /pseudo
     CDS             2660..2950
                     /locus_tag="PC399_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3147..4154
                     /locus_tag="PC399_002913"
     CDS             3147..4154
                     /locus_tag="PC399_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2593759.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            4192..5619
                     /locus_tag="PC399_002914"
     CDS             4192..5619
                     /locus_tag="PC399_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF2593760.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTKKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SAGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQMYMGVEE"
     gene            5761..7026
                     /locus_tag="PC399_002915"
     CDS             5761..7026
                     /locus_tag="PC399_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2593761.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTRIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            7068..8666
                     /locus_tag="PC399_002916"
     CDS             7068..8666
                     /locus_tag="PC399_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2593762.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            8701..9723
                     /locus_tag="PC399_002917"
     CDS             8701..9723
                     /locus_tag="PC399_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2593763.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(10111..11514)
                     /locus_tag="PC399_002918"
     CDS             complement(10111..11514)
                     /locus_tag="PC399_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2593764.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            12381..12890
                     /locus_tag="PC399_002919"
     CDS             12381..12890
                     /locus_tag="PC399_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF2593765.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISSNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            12918..13112
                     /gene="rpmI"
                     /locus_tag="PC399_002920"
     CDS             12918..13112
                     /gene="rpmI"
                     /locus_tag="PC399_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF2593766.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            13168..13524
                     /gene="rplT"
                     /locus_tag="PC399_002921"
     CDS             13168..13524
                     /gene="rplT"
                     /locus_tag="PC399_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF2593767.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            13834..14379
                     /locus_tag="PC399_002922"
     CDS             13834..14379
                     /locus_tag="PC399_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593768.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            14366..14530
                     /locus_tag="PC399_002923"
     CDS             14366..14530
                     /locus_tag="PC399_002923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593769.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLLGLYFIVSFHLS
                     NDLEWYNEVF"
     gene            14819..16744
                     /locus_tag="PC399_002924"
     CDS             14819..16744
                     /locus_tag="PC399_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2593770.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKYTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            17145..19049
                     /locus_tag="PC399_002925"
     CDS             17145..19049
                     /locus_tag="PC399_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2593771.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKI
                     FGKEVNIITTSSLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         7237 a         2141 c         3391 g         6354 t
ORIGIN      
        1 aatagagata caagagatag atatattggt tttaatttta tagaaatata caaagatatt
       61 ttaagctaat atatagtaat ttatatagca ttataaaata gaatcatata aaataagtta
      121 actacaatca aaaattaaaa aggggaaatg ttaagttgat agaaggcata aaatttataa
      181 aggcagaaga aaagtatatt tattcgtatt ggcaaacttt tgataaaata gcaaaagaga
      241 ggaaatatct agctatggat gaagcctttc cttttgaaga aacagttgaa tttataaaaa
      301 atattataaa taaaaactta ccacaactgt ttataattga tttagaatca gataactgta
      361 ttggttggtg tgacgtttta cctaaaactg aaaaagttgg ttatttaggc atgggtattc
      421 ttaaagagta tagggaaaaa ggtatcggaa gtaatctttt aaaacaaatt attgatttat
      481 caaaagaata tgggtatgaa aaaattgagc ttgatgtatt taaaagtaat agtagagcta
      541 ttcatgttta taaatcttta gggtttgtag aggtaaatac aatatctagt ggttttacat
      601 ggaatgatag acctgtaaaa gaagaagtaa ttcaaatgga gcttacactt atataaaata
      661 aagttaaagt ctttaaaatt ccatatacac ttgtataaaa taaagttaaa gcccttaaaa
      721 ttccatataa aagaaatatt tttaagggct ttttaataat gcttcttttg aaggtagcta
      781 aatatctaaa attttatata ttaaatttaa agaaaatata aagatggaag atacaaccat
      841 gtttactaat aaaaaaatta aagtcataaa taataaactc ataaacaact tattattact
      901 tatgtttatt ttgcatctat tacttcatga gccattttaa ctaagtcatt catagataaa
      961 tttgagtctt gaactgtaaa agaatagtaa actccatctt gtttccatgt caaaaactta
     1021 aatttattaa cttcaacttt gtctgattca ttatttggcc ctacactaaa tgtgtatttg
     1081 cctgattttt catcaatttt atcctgttct gtcattttat aaccttctgg tacaaacttt
     1141 tgtgcgaatg cttcataaga aatattaata tctttgtaag tatctacaac ttttagattg
     1201 tcagaactct tcccaagtac attatttgct gtgtcaagag atatttcatc attgccattt
     1261 ttatattcaa aactaagtgc ttttgatttt cctaaaacat tattttcttt gtcaaatgct
     1321 tttttattta cagtatatgc tcctttaaat ttatatccat tactaaattc agaaactata
     1381 ttaggggtaa agttaaagtc tttttttact tgttctgtag ttgggataga tgtataggta
     1441 gccttagtac ttgattttcc aaccaaacta tatatatgtc ctactgcaag ggctgttgtg
     1501 ctcaatatca aagtaatggc taaagttgca actaagcctt ttctaaatgg taatttgtgt
     1561 gttttattat taatcatttt ataattttcc tctctttctg gtaaactatt tagtgttttg
     1621 cttaaactat tatgaaaact ttctggaact tctggaaacg aattatttaa tatattttta
     1681 ttcatatgat tacctccgtg ttttctaatt tagtttttaa taatcttctt cctcttgata
     1741 atctactttt tacagttcct tgaggtatat ctaatatttc cttaacttca tctactgaat
     1801 aaccttcaat atagtaaagg actattggaa ttttaatttt tttaggtaga ctttgaatag
     1861 cagtatacaa atcgctatag tcatcttttt catcaatttt tttgtttttg aagacatctt
     1921 caaatgatag agattttaat cgtttgcgtc ttaatgaatt acattcattt atgactattc
     1981 taatcagcca tgttttaaag tattgttctt ccttaagaga atccaacttt tcatatgctt
     2041 ttaaaatagc attgttgacg gcatcttcac agtcttggtc gtttcctaga atagacttag
     2101 atactcgata caaagtctgt tctgattcaa gtatattgtt tgtaaatgta gttttatcca
     2161 actaagttcc ttcccttcta tgcaaatcgt agcgctactt ttttgtcttt cacttattag
     2221 ataaaatttt actatagatg gttcactttt tttatgatta tttgataaat ttaagtttga
     2281 aagttttgcc tattctatac aaatattcta taatatattt tttctagtat tttcccctca
     2341 tatataaagc catgtagata tattaaactt tagaagccat ttatatattt agttttatag
     2401 taagtcttta tattgagtaa ttagaaaata gtaagtattt aatgttgagt agttagaata
     2461 acaatgcttt tggattattt acttcatgta cagatttaaa ataagttctt taaatagcaa
     2521 ctttttgaaa aaacgcaaaa atattttatg ttgtctgatt atatttatag atgtgaaaca
     2581 atattgtata gagtaaaagt atatagaaga tgattacata gagtttatac ttgaatttta
     2641 caaatataat tatcaaaata ctgtcaaaga gaaaaatagt tttgatgaac gtttttatca
     2701 aattgctaca gagacagggc taagtgctaa tactgtatat aaagtattca ataaaataat
     2761 aaatagtgga aaaactgaat ttacatcaaa ggaattagct actatatgtg gtgtaagtat
     2821 tagaactatg gacaggatta tacttagatt gtgtgattct ggttattgtg aggtagtgag
     2881 tgaacagctt atgagcaagt atggtagacc cagtagaata ttacgtttta aaccttttct
     2941 gttgttttaa atagatataa gtcaaaaatt aaatttttat gataacattt tctttacaat
     3001 tttttagata tatgttaata tttagaaaaa tgcatatgta ttcataaaat aaacttaaaa
     3061 cagctaaaaa atcattaaaa aggaaattgt atgcatgaaa tgagaaagtt aatttataac
     3121 ttaaataaat ctgggaggag ataattatgg gaaaatatca ggattcaaaa aaagttgtaa
     3181 gaaattattt tgaagcatta gaaaatgcta ctggggatga ggttgaaaat gtcttaaagc
     3241 aacatatgaa atcagattat aactggaaag gtgtgtatcc atttcgtgaa caagaaggca
     3301 ctgaaaatgt agcagatgta ttttggaagc ctttaaaaaa atcattgagt aatatgcaaa
     3361 gacgacaaga tattttcatt gcaggaacaa atgagattga caatagtgaa tgggtaatga
     3421 gtatggggca ttttatggga ctttttgata acgattggct tggcattaaa catactcgta
     3481 aaatgattag tttaagatat gcagaattta actgtgttca agatggtaaa attgctaaaa
     3541 ctggattatt tgtagatata attgggttta tgattcaagc aggagttaat ccattgccac
     3601 cacaaacagg ttcatatttt gtatatccag ggccaattga ccataatgga ttactttttg
     3661 aagatgcaga tgaagttgaa ggagtaaaaa ctttagcatt agtaaataaa atggtagatg
     3721 atttatctga attaaatgaa agtggtgcta tgggatgccc accagaaata cttgaaaaat
     3781 cttgggctaa agatatgatt tggtatggtc caggtggaat tggtgcttct tatactatac
     3841 ctagatatca agagcaacac cagctaccat ttagaaataa tttaaaagga aagactttca
     3901 atgggcatgt atgtagattt gcagaaggta attttgcttg tttctttgga tggcctaatc
     3961 tgactaatgt accatgtgga ggttttttag gattaccagg tggagaggtt aaagcagata
     4021 tgcaagttgt tgatgtttat tatcgtaagg gcgataaact tcaagaaaac tgggtgttaa
     4081 ttgatattcc ttattggttg aaacaacaag ggcttgatat tcttgagaga actaaaagta
     4141 tttttaatgc ttagttatgc atacgtattc taaaataaag ggggatataa agtggcatct
     4201 accaacaaaa ataatgcaac aagtaaagaa gaagaaaaag aagtttctac aagtgggctt
     4261 gaatcatgta cggttaaaga aaacaaagct aaaatgaatc aaactaattt tagtaatata
     4321 aatgaaaatc aaacaaatga gaataaaacg aacacatata aaagaaatat agataaagga
     4381 aatatagata aagggaatat agataaaaca aacacaaata aatccgacat aaataaagtt
     4441 aacatagata cagtggatac agataaatta aatacaaata ctcttactaa aaaaaataaa
     4501 aaagttatat ttaaggggga agataatatg tcatctacaa aaatttacta tggagattca
     4561 ttagaagtaa cgcctgttgg agtaatggat tacaatgatt tttcaaaaca gacaaaaagg
     4621 gaacaggaaa tacctggatt tgattctaag tatagagatt ttgtagatta tattatgaag
     4681 attacacata atatctggga agaaaaaggt ataggtgtta tttatgatac atatcacaat
     4741 aatgttacaa tgcattgtgg ctcttcaaat ttagttggta ttaaagatgt tatttccaat
     4801 actcttcaaa cattacatgc gtttcctgat agaagattaa ttggtcaaaa tgtaatatgg
     4861 tctaactttg gtgctgatgg ttttttatct tctcaccgtg ttttgtctac agcaactaac
     4921 ttaggtgata gtaattttgg accagcaaca ggtagaaaaa taaactttag aactgtaatt
     4981 gattgtgcca caactaacaa tagaatccac gaagaatggt tggttagaga taatttatgg
     5041 attgttactc aattaggatt aaaccctcag gaagtagcaa agagtatggc aaaggcaagt
     5101 gaatctaaag tgcttagttt acaatcaact tatggtatct gtgaatctat ggatggtcaa
     5161 tttatgccaa ctaagtatca agcaaaagat gattctgctg gcgaaatgat gcttgaaatg
     5221 acaagccgta tttataatta taaatatata aatgaagtta aaaaatacta tcatgataat
     5281 gcagtagtac attttatatg tgataaagat ttaaatggtt atgatgagat tcaaggaatg
     5341 attgtaagtt tacttgcatc aattccaaat ggaagttatg aagtggaaag agtaacttgt
     5401 aatcaaagag aaaaaaatga aggttatgat gtatctgtaa gatggagatt acgtggaata
     5461 aatgaaggta taggattttt tggacagcct tctggaaagc atatagaagt aatgggaatc
     5521 aatcattatc atatactaca aggtaaagta aaggaggagt ggataacttt tgatggtatg
     5581 gatgtattaa aacaaatgta tatgggagta gaagaataaa gctgtttaga gtataaatag
     5641 ttttgaaata tatagggaag tattgcttta taaaatttaa gtggggaatg tatatgaaaa
     5701 atagatgttt aaacttttag caaaattatg actaaattta aaagggggaa ttataaaaat
     5761 atgaaaacaa cggttgaaat agaaaatgat atcatattag gaaaagacta ttcaaaccta
     5821 aaaagaggaa ttgcattttt ttcaattgct ttaatatact ttttttattg ctacaatttt
     5881 atggtgggta cttttattaa accaacaatg atttatgctt tagcagatgg gggatttggt
     5941 ttttcattaa aacaaacaga agaaatattt gcagttatgt cttttggtac aattccaggt
     6001 acatttattt ttggggtgat ttctacaaaa attgggaaga agaggacttt aattagcgtt
     6061 gctttactga ttggattgac aacatttata ccaatgttat ctcctacaaa tattatgtta
     6121 tggaaaatag cacgtttgtg tacaggagta gtgttgggag gcgtatttgg aacagctatg
     6181 cctttagttg cagatatgtt tccaagtaaa tatcgtggta agttagctgc tatattaact
     6241 gctttatttt cattagcaat gatttttggt gggaaagtat atggcgtttt aggtgatgca
     6301 aattggcaaa tattgatgta tactgcgatt ataccaccta tagttggagc tatcttagca
     6361 attttctttg tgccagatga tttggagtat acaaaggaat tagttaaaaa gggaaaagaa
     6421 actggtgaaa agattagcta tataagtatg tataagggaa aatacttatg gattggattg
     6481 ggaacgattc ttttatcagg tgcaaatttt acagcatatt cagcattttc aaataatgca
     6541 actacatacc tagtgacaag aataggaatg tcagcagcag tggctgggtc tatatatagt
     6601 ttacaaggaa ttggtcaatt agtaggatat ttattctggg gttcaattgc agataaattt
     6661 ggaagaaaaa taccttttat aggtatggca ttagcagcag tatttgtttt tatatataca
     6721 aaattaggtg gagataatac aacaacattc tatatgatat cagtattact aggtgttgct
     6781 gttggatatt ctggagcatg gggagcatac tacacagagt tgttcccaag taaatatcgt
     6841 tcgttgtcat ctggtatgtc atttaatgga ggaaggatta tatctacgtt tgcaatacct
     6901 gctatagctg gtacagcttc tggtacttta ggtatgatga ctatctttta catatcaatc
     6961 gcagtctttg tagcaggtgc tattgtatgg ttattcttac cggagacgtt aaataataaa
     7021 aaataaaata ctaggaattt attttgttac ttagaaaggg gtggcttatg atttcatcaa
     7081 ttgatgtatg tattataata ggatatttgc tattaatgtt agttattggt tattattcag
     7141 gaaaagataa taaaaatcaa gaagattatt tcttggcagg taggtcaatg ccatggattc
     7201 caattgcttt atcagttgca gctactatga ttagtgcaaa tggctttata ggtggtcctg
     7261 gatgggcata tgtagatgga atgtatccag taatggttaa tattacagta cctttagcaa
     7321 tattttttgc actgagcatt accaccccag ttatctataa attaaaaata acctctatat
     7381 atgaatatat gggacttcgt ttaggaaatt attcaagagg attaactatt attcaatttt
     7441 ttattaattc attgattcaa gctagttcta tggtatatat tccagtgctt attattcaaa
     7501 tgattactgg ctggtcactt tatgctttag tcccaatagt tgtacttgta tcaattatat
     7561 atactctatt aggtgggatt aaggcagtta tatggactga tagtattcaa atgatagtag
     7621 taataagtgc tgtatttata gtaattattg tagccttaaa aggaatgaat ctaagtttct
     7681 ttgatacatt acaaattgca caacagagtg gaaaattaaa tacatttgat ttcagtaggg
     7741 atatttcagt gacaaatact ttttgggcta ctttaattgg tggaactgta atgtggataa
     7801 gatacttttg ttttgaccaa tctcaagtac aaagagtttt aacatctaaa tctttaaaat
     7861 gtgcaaaaaa ttcttttgta gttagtgcat ttgttatgaa tttagtttac tactttatgc
     7921 tattggtagg agtaatttta tttgtttttt atgggggaag gacatttgaa acttcaaatg
     7981 aaataatgat tacttttatt ttaaatgaac ttccagtagg agccattgga attattattg
     8041 caggtgtgtt tgcagcagca atgtctagta tcgactctat attaaacagt atgacaactg
     8101 tttttacgaa ggatatttat gagttttatt tcaaaaaaga caacaaagaa tcatcattaa
     8161 aagttacaat gattataact gtagttatag gagttgttat gacaggtttt attattatgg
     8221 gatttggagg aagcatcaaa tcaattcttg accttgttgg taactatatc tcatactttg
     8281 ctggaccagc tacaggtgca tttgtactag caatgtttac atataaagct aatgataaag
     8341 gagtatctat tggttttatt gtagggttag ttttaggatt ttttatttct aataaatata
     8401 atgtgagttg gttgtggaat ccagcaatag gtgctacaat aacattgata tttggatata
     8461 tttttagtgt cattttaaag aaaaatcata atgcagatga tattaaaaaa tacactgcat
     8521 ttggtatgag aaatcacctt atttctcaag gaatggataa agtagatgga gtatctatag
     8581 tgccattttc tttaggtaaa caagaactaa ttgtattagc tttttttatt atccaatata
     8641 ttatattatt tttgatacaa ttttaagaag aaatactaga tatttaagga gaaatactaa
     8701 atgaatcaaa agaaagttca ttataataaa tcggctacct caaaagatgt tgcaagattg
     8761 gcaggagtat ctcaatcatc tgtttcaaga gcatttggta gtgcaaatgg aaagggcgta
     8821 aaacctgaag taagagaaag aatttttcgt gtagcaaacg aaataggata tgttcctaat
     8881 ttggtagcac ggggaatgat tagtggaaaa actaatgtta taggattgat agttggtgat
     8941 agtctaggtc cattttacaa tagaattatc aatttatttg tagaaaaaat tcaagagata
     9001 ggtaaacagt gtctcgtttt taaagttcct agacaacaaa gtatagatag tataatttca
     9061 aaagtaattc agtttcaagt agaagctgtg attattacag cttcggccat gaataaggtg
     9121 atggctgaga cttgtgaaga aaacaatatt cctgtgattc tttttaatcg ttttatacct
     9181 ggtataaaaa taagtacagt ctatgtagac ccaattgaag gtgggggaat ggctgctgaa
     9241 tatctgctta agaaaggtca taagaatata ggatatattc aatttacgag agaaacaagt
     9301 gaagaaatgg aaaagaaaat tggattttat tctaagttaa gacaaagtgg gattcataac
     9361 cttaaagaag aatctgcaaa ctatgattat gattctggat atgaagcagg taagagaatg
     9421 ttagcattat caaatcctcc aacagcaatt ttttgcacaa gtgacttgat agctattggt
     9481 gtaatggatg ctgcaaggtt tgagtacaag ttgagggtgc cagaggattt atcagtgata
     9541 ggatatgatg atattcaaat ggcttcatgg aagagttata atttaacgac gattagacag
     9601 ccactagatt tgttgattga aaaaacaatt aacatactta agtccttatt aaatgaagaa
     9661 aatccagagt cagttgttga aatgttaaaa cctgaattga ttgaaagagg ttctacactt
     9721 taagtggtag atatttaatt aagtggtaga tacctaatta agttgtagat atctgataag
     9781 aaatttttaa gttttcaatc gttctaaatt gtgagtataa aaatctacta aatttagtta
     9841 tgtaaaatat aaattataag tgtagaaact aggctactag cttttatacc acttcaaaca
     9901 ttactggagg tagttaagtt tacatcagtt tagttttatg tagatataat attctgtaaa
     9961 tagacaaaat taaaatatgt aagtgtctca aaatgaaatt tttagtttat gaggcacttt
    10021 tttgtataaa atcaaatgta tttttataac tttaacaata aaaagaggct tatctctatt
    10081 ttgagacagc ctcaaatttt taaaactata ttagtttaca gcttcttttc ctatcaaatc
    10141 ttttaactct tcatgagtca tagtttcttt ttcaaataag aatttagaaa cagaatgtaa
    10201 atcttcaaga ttatccttta acatctctaa agtctcttta taacaaattt caactatacg
    10261 atttgcttcc ttttgaacct tatcacataa gaaagtctta tcatttccat caatagtcat
    10321 aaatcctaag ttactcatac catattcaca aaccatttgg ttagctattt cagtgacttt
    10381 ctttaagtcg tcctttgcac cagtagattt atgattaaat attaattctt ctgaagcctt
    10441 tccagcaagt aatattttta tttttccaat caactcatcc ttagtataaa tatatctatc
    10501 ttcatcaggt aattgaagaa cataacctaa tgcttgtcct ctaggaacta tagatatttt
    10561 ttgtatagga caaattccta ctatatcact tacaagagca tgtccagctt catgatatgc
    10621 tactattttt ttctcttttt caaccaatgc agagttttta gattctagcc ctgcaataac
    10681 tctttcaagt gccttgtcaa aatgttctga tgtaatacac tcactgtcat ctctaactgc
    10741 aaatatagct gcttcatttg ctatatttga aagatgagcc ccattgaaac catgagtttt
    10801 tttagctaaa agttctagat ttacagattt gtctataggt ttgtcatctg tatgtacctt
    10861 tagaatttca aatctagtgt gatagtttgg tgcacctata tgaatgtgtc tgtcaaatct
    10921 tcctggtcta agtaaagctt catctaataa gtcaagtcta ttggtagcac ctattattaa
    10981 tacattggaa tctttattaa atccatccat ttcaactaaa agttggttta gtgtttggtc
    11041 tttttcgttg ttactttcaa gatgtctctt tgctcctaca gcatctattt cgtctataaa
    11101 tattatactt ggagcttctt ttctagcttt ttcaaataga gttctaactc tttttgctcc
    11161 cacacctaca tatttttcaa cgaactctga accagtaaca ttgaaaaatg atgagtttgt
    11221 ttcaccagct acagcagatg caagtagggt tttacctgtt cctggaggac cataaaataa
    11281 tactccttta ggtatttttg cacccatttt ttgatatttt tgtggtgact tcataaaatc
    11341 gactatttca aatagttctt cttttacttc atctaatcca gcaacatctc taaatgtagt
    11401 ctttggtttt gtagaaagtg aatcatctgt ttgattatct ctttccattt ttgtctgtgc
    11461 aaagacagga gctggattgt tgattttctt aaaaaagttg tttagcattt tcatattcac
    11521 acctcaggta attaattatt gttaatcaat tttatatttt ccatatttac tattttttat
    11581 tatttatttt tcatggaaaa aatatacatg ttatctaaat aaaaataaat ttgtaagatg
    11641 tatgaaaaaa tgaatttatg tatatagaga tttctactta tgtattgaat aaagtattac
    11701 aaattataaa ttatgtattg attatatatt gagtaaagta ttataaagca tgtattgatt
    11761 aagtattgaa taaaatatta caaatttagt tgaaatgaaa atgataaaaa atcttgtact
    11821 atattttaca aatcatataa atagagaaat tgttgtagca caaagtagta ggtagtatat
    11881 tagaatataa tataggattt gttgtgtagt atagtagcat attagaatat aatataggat
    11941 ttgttatgta gtatagtagt atattagaat acaatataga atttgttggg tagtatagtg
    12001 ttatattaga atacaatata ggatttgtta tgtagaatat atagagtcat gtagaatata
    12061 aatatataaa aggattttgg gtataaattt aaaaattaat agcaaaatga gtagtataga
    12121 catataatta gtagtaggaa gtaattaaat ttttcctaaa caaaaaaaat agttgcaaat
    12181 aatttgatta agtgatatag tagataaggt aagaataaaa caaagaagaa gtccgcttct
    12241 caccttacgc cggaggttag taaggtaaat ataagttaaa gcttaatttt aatttaagtt
    12301 tgtttaaatt tggtaactta tattgcggat gattacgtca tccgcttttt attttataaa
    12361 aattttggag gtgtcctaca attagcaaag aattagcaat caatgaacaa ataaaagata
    12421 aggaaataag agtcctttca ccaacaggtg aacaacttgg agtaatgcct actaaagaag
    12481 ctcaagcaat ggctaatagc aaaaacttag atttggttca aatctcgtcg aatgcaaatc
    12541 caccagtatg taagataatg gattatggaa agtttagata tgagcaagcg agaaaagaga
    12601 aagaagctaa gaaaaaacaa aaaactatag ttgtaaaaga agtaagactt agaccaggta
    12661 ttgaacaaaa tgatttaaat acgaaagcta ataatgctat aaagttcctt aaaaaagggg
    12721 ataaagtcaa agttgaactt agatttagag gaagagaatt aggacataaa gacataggta
    12781 aagaagttat gcttaagttt ttagatataa taaaagagtt tggagaacca acaaaagccc
    12841 ctgcatttga gggtaataat atggttgtaa ttatagatcc aaaaaaatag atgattaact
    12901 gagaggagga atttgtcatg cctaaaatga aaactcatag aggtgcagca aagagattaa
    12961 agaaaacagg aactggaaag ttaaagagag ctaaagcttt taaaaaacat atattaacta
    13021 aaaaatctgc aaaaactaaa atgaacttaa gaaaatcaac tttagtaagt gatggagatg
    13081 ctaagagaat agcacaatta ttaccatact aagatacaga ttgtaaataa taactataga
    13141 agagggatat aaaaggaggt cataacgatg gcaagagtta aaaaagctat gaacgctcgt
    13201 aaaaagcata agaaaatatt aaaacttgca aaaggattca gaggatctag aagcaaatta
    13261 tatagaccag caaatacatt cgtaatgaaa gcattaaaaa atgcttatat aggaagaaag
    13321 ttaaagaaga gagacttcag aaaactttgg atacaaagaa taaatgcagc tgctagaatg
    13381 aatggaatat cttattcaag attaatgaat ggattaaaat tatctggtat tgaagttaac
    13441 agaaaaatgt tatctgaaat ggctatacaa gacccagaag gatttgcaaa attagcagaa
    13501 gttgctaaag caaaattagc ttagtattga atcatgagat gtaaagtctt tgagttatat
    13561 aaataattca aggacttttt tattttttga aaaatactta ttagtgtcat gtacaaccaa
    13621 ttttataatg ataaataaaa taagcaatac aatagaattt tatgagattt attataagaa
    13681 ttgttacaat tatttatgat agctataaaa agtcatagta aaaatgatat tgatgtatgt
    13741 tgatatatgt ataaagcttt caatcaatct tgaattaata ttatcaacat gataatatta
    13801 tcttattaat aatataaata agggggagat aatatgaatg aaaaactaat ggattgtttt
    13861 gtaaacccaa ttaaatgcaa gttattgatt gaaatttttt caaataatcg aactacagct
    13921 aaacaattag gagaaaaatt tcaagatata tcaaaggcaa cactttatcg ttaccttaat
    13981 aaaatgttga aagatgatat tataaaagta gttgaagaaa ataaaataag gggaactgtt
    14041 gaaaaagtat atgctatgaa gttagatata gctgatgatg tgaacaaaat tatagatgag
    14101 aactctggag aagaatatgc tcaactgttt actcaatata tgatgggatt tttaagagaa
    14161 ttttatgaat acgctgaaag aaaggaaaag gatatcctaa aagatggttc aggattttct
    14221 gtctgtccag tatatgctag tattgatgag atagttgaag tgagtaaaaa aattgctagt
    14281 atactagaac ctctaagaaa taactcgcac gaagaaggca gaagaatgca ttctatagga
    14341 gtgataatca ctccacctaa agaggatgaa gggaaataaa tctgatttac atgaggtaga
    14401 gccaatttac acggaaaatc gttttatgat ttacatatgt atccagagca aagtatctct
    14461 gcttggattg tacttcattg tatcatttca tctatctaat gacttggagt ggtacaatga
    14521 agttttttaa tttttcatat caatgcctaa agaaatgaac tttagaccac atatgcaatt
    14581 aggttacatt atacaattag accagatata caaaataaaa atcagtattt ttatagcaga
    14641 taaattgact gttattcttc ttaaaaatca tctattagtt attgttagca tattgaaaga
    14701 aattatagga atctattgaa taaatgaata ttgcccataa tcatataaac tgctatagtt
    14761 taaaataaag caggacgtta aattttatag taatctaaaa atttaagagg gggattttat
    14821 gaaaataaaa tttgttatta tgccattggt gatattttta atggtattat ttgtggggtg
    14881 tcaaagtaca aaagatacag aagttaaagc aaaatataca gaaattaaaa tattaggtac
    14941 aggtgatttg cattcaattt tgacagactc tatggtttca tatgtaaatg aggaaaggga
    15001 aaagaataaa aatctgctta tggtagatgc gggagatttc tatggtacac aaagtagaga
    15061 aatgtgggaa tggtcttctg gaaaaaagtt aattaatatt aaaagaaatg gtagagcaga
    15121 gtatgatgac ataattaaga gtagtaaaga tgaagtacct atagttaaag atatggcaaa
    15181 gttgaaatat gatgctgtag ttttgggaga taatgaattt gtttcaaatg ataagcaaag
    15241 tcttgataaa ctagtttctg attttaaaaa taataatatg ccattggtat cagcaaatat
    15301 atatgaacaa tctggagaaa attatgtaca accatatgta atgaaaaata ttaagacaga
    15361 tgagggcaat gtaaaagttg gaatactagg tcttactata aaagaagtag gggaaaccct
    15421 tggtcttgag gattttgaaa aggataaaaa agctagagag cttggagaac aagctgatta
    15481 caaaggaaaa ttatatgcaa atgatttagt agaagatgca aagaaatgga taaaagttat
    15541 ggaaaaagaa agtccagaca taatagttgc agttgtacac tcaggggaag aacctaaaac
    15601 agatagaaat ccaggaaata gaataaaaga gttagcaact acagtggatg gaatagatgc
    15661 aattgtagct ggtcatactc atgaaaaaat agatgaacac aaatataaaa ataaatctgg
    15721 agatgaagta attgttacac aaccaggttg gcatggagat agagtatcag agattaattt
    15781 caaattaaat aaagagaatg gaaagtggga cataaaggaa aagtctagta aattaaaaat
    15841 aattgataaa gaagtaagta tagttgctac tgcagattta cattcacatt tctctgataa
    15901 attaacagtt gaccttgtta atgaacgtag taacccaatt gaacctgttg tagtagatgc
    15961 aggcgacttc ttagaccctc aaacagatga aatgactcaa tggtataaag agtggaaaag
    16021 tataagagat aataatagtg ataaaacgat tagtagatgc ccaatggtca tggatatgaa
    16081 tcagggaatg tatgatgcag tagtacttgg aaatcatgaa tttgtatccg aaaaagatga
    16141 attttggtca gatgaacgcc ttttagatgc tgttatagag gattttgaac aggtggcaat
    16201 ttctgtgtta tcggcaaata tatataggca atctggtaaa aattatgtta aaccatatat
    16261 tataaaagat gtggagacga gtgaaggtaa tgtaaaagta ggaatattgg gacttactat
    16321 aaaagaagta ggtaaaggtc ttaaaaatgt aaacttacaa gagcaatttc aatataagga
    16381 taaattgtat gcaaatgatt tagtggaaga tgcaaagaaa tgggtaaaag ttatgaaaaa
    16441 gaaaaaacca gatataatag ttgcagttgt acattcagga gaagaaccaa aaaatccaaa
    16501 acatccagga aatagaataa aagaactagc aactacagta gatggaatag atgcaattgt
    16561 tgctagtcat actcatgaaa aaatagatga gcatgaatat aaaaataagt ctggagaaaa
    16621 agttatagtg acacaaccag gtgagcatgg taaatattat tctaaaatta cctttaaagt
    16681 aaataaagaa aagggttcgt ggattattaa tgataaattc agtaaaacaa ttgaggttga
    16741 ataactttat aaaatttata gataaagtaa attatctgat attgacatga tatataagat
    16801 aaagtttcta atattagtat aatgcataat gtgaaatatt ttaataataa gagaataaac
    16861 aagacaaata aaaatatttt aacatgtaag atatagtatg aactattata ctaatgctta
    16921 ctttataatt tgaacatgtt agaataagtc ttatttgaaa attttagtgt attagagtaa
    16981 gtattatttt accagtaaaa aatattgaga actatatgta gatttgctta aaaaggaatt
    17041 tacatatagt tcttttcgta taataaatag tacaatctta tatggtaaag ataaagtgtg
    17101 atattaaatt ttattatcat ccaaaatcta aggaggagtc tcttatgaaa ataaaattta
    17161 ttatagtagc atcaataata tttttaatag tattacttgt ggggtgtcaa agtactaaag
    17221 acacagaaat caatgtactc gccacaagtg atttacattc aattattcca gataatttaa
    17281 tttcctatgt agaagaggag agaaaatatg atgagaatct acttatggta gatgcaggag
    17341 atttttttga catgcaaagt ggtgagatga ataagtggtt tactggtcaa aaattagtaa
    17401 gatttaaaga tgggatacca gagtacaaaa aaatagctga accacgtgaa ggagaagttc
    17461 cattggctaa aaaaatggca aagctgaagt atgatgcagt tacatttggg aatcatgaat
    17521 ttgtatctaa tgacaagcag agccttgata aattggtttc tgattttaaa aataacaata
    17581 taccattagt atcagcaaac gtatatgaac aatctggaga aaactacgta aagccatatg
    17641 taatgaaaaa tataaatact gacaagggaa ctgtaaaggt tggtatactt ggtctgacta
    17701 taaaagaagt aggggaacgt tcaaggttta aaagttttga acaagataaa aaggctagag
    17761 aacttgggga acaacctcaa tacaaaggga aattatatgc aaatgattta gtagaagatg
    17821 caaataagtg ggtaaaggtt atggaaaaaa aagaaaagcc agatataata gttgccattg
    17881 tacactcagg tgaagagcca aaaaatccta aaaatccagg aaatagaata aaagaactag
    17941 caacaactgt agagggaata gatgctattg tagcaggtca tacacatgaa aaaatagaac
    18001 agcatacata taaaaataat tctggtgaag aagtaatagt aacacagcct ggagctcatg
    18061 gtaatagtat atcaaagatt aattttaagt tggataagaa gaatgataag tggtttataa
    18121 aagacaaata tagtgaatta aagatttttg gaaaggaagt aaatataatt acaacttcta
    18181 gtctgaatcc aaaattttca gatgaaatgg cagttaattt gtttaatgaa agagatagag
    18241 atgttcaacc tatatttata gatactggag attttttaaa tactaataca aaagaaatga
    18301 ctaagtgggc taaagagtgg caatacaatg aaaataaagg catctataaa aaaataaatg
    18361 aatggccaat tgtatatctt ggttatgatg ctgtagtact tggtagccat gaatttacag
    18421 aggataaaga gacttttgag gaaaaaaaat ctacattgga ttatattata gaaaactttg
    18481 aagaaatgaa gataccagta ttatcagcta atatatatga tgaatctgga gaaaattatg
    18541 taaaacctta tataattcat aatgtagaaa ctagggaagg aaatataaag gttggagtat
    18601 taggtcttac tactactgat aaggatgaag aagacttaag tagctcaagc ttaagcaaag
    18661 actcaaagac ttcgaaaatt cctgaagaaa caggaaagtt atatactaat aatctagtaa
    18721 aagatgcaag cgaatgggta aaagttatgc aaaaggaaag tcctgatgtg attgttgcag
    18781 ttgtacattc agataataat agtactctaa aaaatccaag tagtgaagtc aaaaaacttg
    18841 caatgtcagt agatggaata gatgctattg ttgtaggtca tacgaaaaat aaggtagaag
    18901 aacatattta caaaaataag tctggggatg aggttatagt aacacaatct ggagaagatt
    18961 gttattcaaa aataagtctt gaactaagaa aagaaaatgg caagtggaat atcataaaca
    19021 aatatagtaa agcaataaaa atgaagtgaa tttattgata tataaaccat tattaacact
    19081 gatttttata atataataaa tactgattct ataatataat taa
//
LOCUS       DAMJWS010000081        19013 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMJWS010000081 DAMJWS010000000
VERSION     DAMJWS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.082"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            86..1687
                     /locus_tag="PC399_002926"
     CDS             86..1687
                     /locus_tag="PC399_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF2593772.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASNSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLLVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1906..3504
                     /locus_tag="PC399_002927"
     CDS             1906..3504
                     /locus_tag="PC399_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2593773.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTIILVNTDNSIVDGLSASGLSGVAKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKANIKGNVDINKAGTYVLNISVVDNLGQKSEISVNVKVVVNASTKDSNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKSLRELDSLAGMANAWSKYMEDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYTSKDAKEMAESIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            3794..5992
                     /gene="slpA"
                     /locus_tag="PC399_002928"
     CDS             3794..5992
                     /gene="slpA"
                     /locus_tag="PC399_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF2593774.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGK
                     YVDFKITYGAKAEVPAASLSADDIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNK
                     VIAGDAPLKVKDAVKAIVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASSSDVDAIT
                     LDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAV
                     EKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVT
                     IFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQT
                     VAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAP
                     LLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLK
                     VTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVV
                     DGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNA
                     EVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPA
                     PIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            6238..8580
                     /gene="secA"
                     /locus_tag="PC399_002929"
     CDS             6238..8580
                     /gene="secA"
                     /locus_tag="PC399_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2593775.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEDKIQILDDEALKNM
                     TNVFKDKLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     PVERKLQYQCDITYGTNNEYGFDYLRDNMVTQKSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKNIRDEDFEMDRKDKTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMNIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVEEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDIEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
     gene            8994..10865
                     /locus_tag="PC399_002930"
     CDS             8994..10865
                     /locus_tag="PC399_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2593776.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAEATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
     gene            10974..11681
                     /locus_tag="PC399_002931"
     CDS             10974..11681
                     /locus_tag="PC399_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2593777.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            11714..13846
                     /locus_tag="PC399_002932"
     CDS             11714..13846
                     /locus_tag="PC399_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2593778.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKSISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTDLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETIYRV
                     KTADELDVALRKADANDIITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVNMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
     gene            complement(13899..14294)
                     /locus_tag="PC399_002933"
     CDS             complement(13899..14294)
                     /locus_tag="PC399_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF2593779.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELIFLFKEFLFFVSFRLLSGIIEMIIMYIMIDLFF
                     VNDITVKVFTNIIVIVLNYLFSKMIIFRK"
     gene            14521..16932
                     /locus_tag="PC399_002934"
     CDS             14521..16932
                     /locus_tag="PC399_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HDF2593780.1"
                     /translation="MRKYKSKNLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            17229..18806
                     /locus_tag="PC399_002935"
     CDS             17229..18806
                     /locus_tag="PC399_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF2593781.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         7738 a         2123 c         3585 g         5567 t
ORIGIN      
        1 actctataat atatggaaaa taaagagaaa ataaatggaa aataatcaca atataaatat
       61 aaaatataaa aatcatcagg gggatatgaa aatgaacaaa aaaatattat cattaggtct
      121 agcagtatca ttaatcttag taaacttcaa aagtgtaaat gcatcatcag tagtagaaaa
      181 aatatacggt aaagatagat atgaaacagc agcaaagata gctgataaac agacttatga
      241 aacagtaatt ttagtaaata cagaaaaatc acttgcagat ggattaagtg caagtggatt
      301 atcgggagct acaaaagctc caatactatt tacacaacaa aataagatac cagcagacac
      361 aaatagatgt ctaaaaaata tcaaaaaagc atatataata ggtacagaag atactataag
      421 taaatcagta gaaaaagaac tagattctaa aaatatagaa gtcaaaagaa ttggtggaga
      481 agatagacta aaaacaagct atttaatagc taaagaaata gcaactataa aaaaagtgga
      541 taaagtacta ttaactaatg catatagtgg agaagcagat gcaatgagtg tatcatcagt
      601 agctactaga gatggagctc caatcatact tacagatgga aagagtgtgc cttttgatgt
      661 aaaaaatatt caatcatatt gtataggttc agaggagata atgagtaatc ctctagttaa
      721 aaatacaaac tcagtaagaa tagaaggaac tgaccgattt gaaactaaca aaaacgtaat
      781 agactacttt tttaatagtg cagatggatt ctatgtatca gatggatacc aattagtaga
      841 tgcaatagca gctgcaccac ttactaaaaa ctctccaatg gtactagtaa atgatggaag
      901 cgataaaact gtattagaag gagctaagaa tataacctct gtaggtgaaa taaatgagaa
      961 ggtaatacaa cagtgtataa atgcttcaaa ttcaaatggg caacccccaa caattacagt
     1021 tggaagtaca gaggtatata agggtgaaaa gtttgacact ggcaagttaa atatagtagc
     1081 taaagataac acagggaagg tattaccaat agaagttgat gggtttatag atacaaatag
     1141 agtaggtaca tacatattga cattaaaagc tacagacgaa tggggaaaaa gcacaggaaa
     1201 aagagtagaa ataaaagtgt tggatgacaa atctcatgat tacaacagcc cagaatttaa
     1261 aaaaatggta tctactgaaa tgtataactt aatcaattcc tatagaaaag aaaaaggcaa
     1321 agagcctttg ctagtgtcta gtaggctaga aggtatggca aatgcatggt ctaagtatat
     1381 gatggataaa aaagtatttg cacattatat agatggaaaa aatgctccac aagtattttc
     1441 tgaatttgga atgagaagtg aggaaaatat agcatatatt tatatagatt ctaagaacgt
     1501 tcaaacgaca caagatgcaa aagacttggc aaaggctata tttgaggtgt ggaagaagtc
     1561 tccagagtac aatgcaaaca tgcttagtaa tgagttttat agtactggat ttgggcttta
     1621 tatattatct gatggtcaag tgcatgctac tcaggagttt ttaaatggaa atgaaggtag
     1681 cttgtaaaaa attccataaa tcttgttttt ttgttaattt actgatatta taaaatgaaa
     1741 atcacatcta aagtactttt tgtaatctgt tttgttattg ctttacaggc tatggtattt
     1801 taggtgtttt ttgttttagt agtttataga ttttgaggga atcactactg aggatgtaat
     1861 ttatgattag ctgaattata taaaaaaatt agtaggagga gtttgatgaa ggtaaataag
     1921 agagtattat caataggatt gacaatatca ttaataatgg caggagcacc aaatatcaat
     1981 gcactttcga gcatagaaaa gatacagggg aaagatagat atgagacttc tgcattgata
     2041 gcagataaac aaatatatga tacaatcata ttagtaaata ctgataattc tatagtagat
     2101 ggattaagtg caagtggatt atctggagtt gcaaaagctc ctataatgtt ggtgcaaaga
     2161 gataagatac caacagatgt tgaaaaaaga ctaaaggatg ttaagaatgc ttatgttata
     2221 ggtacagagg atacaatagg taaatctgta gaaaatcaac ttaagaataa agggattgaa
     2281 gttaaaagaa ttggtgggga agatagaata aagactagct atcttatagc taaggagatt
     2341 tcagctatta aaccagtaaa tgatggtgat aaggtttttc ttgtaaatgg atacacaggt
     2401 gaggctgatg caatgagtgt atcttctgta gctgctagag atggagttcc tgtgatactt
     2461 actgatggaa agagtatacc atttaaagtt gatggcgttc agtgctattc tcttgggtca
     2521 gaggagataa tgagtaatga acttgtgagt aagactaatt cagttaggat tgctggtaag
     2581 gataggtttg aaactaataa gaaggttata caacgctttt ataaaggaac aaagaagttt
     2641 tatgtttctc aagggtataa gttagttgat gctgtggctg gttctccgtt ggctaaggac
     2701 aaacctatag tacttgttaa tgatggaagt gataagagtg tgcttagagg tgctgatgaa
     2761 gttacttctt taggtggaat ggataagaag gttgttgacc aatgtattag ttctgcatct
     2821 gataaaaata caatgcctac aattactgct aatgatgtag agatttctgt aggggataag
     2881 tttgataata gtatgttgaa tatagttgct actgattatt atggaaatga tttaaaggca
     2941 aatattaaag gaaatgttga tattaacaag gctggaacat atgttttaaa tataagtgta
     3001 gtggataatt taggtcagaa gagtgagatt agtgtaaatg ttaaggtagt tgtaaatgcg
     3061 agtacaaaag actctaatag ttatgagttt aaggctatgg tatctaatga gatgtatgat
     3121 ttagttaatt catatagaaa agaaaaaggc aagaagagtt taagagagtt agactcttta
     3181 gctggaatgg caaatgcatg gtctaaatat atggaagata agaaggtatt tgcccatgag
     3241 attgatggta aaaatgcagc tgaagtattt tttggctttg gagcaagaag tggtgaaaat
     3301 attgcatatc taccaatgaa tgttaaatct gtttatactt ctaaagatgc aaaagaaatg
     3361 gctgagtcta tttttgattt atggaagaag tcttctaagt ataatgaaaa tatgttaaaa
     3421 gaagaatttt atagctttgg atttggtatg catgtttcta gtaaaggaga agtgaatgcg
     3481 acaatggaat ttttaaatag ttagatttta tattaattta cattggaaaa gatatagtaa
     3541 atttgtaaaa ataaatatat aaaattaaaa taacttataa aattataaaa aataatttaa
     3601 aaataataaa aatgatgaaa aaacaattaa tttgttggaa aaaacttcaa ttaattttgc
     3661 ataaattatt gaatttatat tttatatagt ctatcataag agtgtgagaa atatttggat
     3721 tatatgaaca tggaaattta atgtacataa ttaaagagat gtaattaata taatgttggg
     3781 aggaatttaa gaaatgaata agaaaaattt agcaatggca atggcagctg ttactgttgt
     3841 aggttctgct gctccgatat ttgctgatac tactgttaaa gaagaaggat atacggtagt
     3901 acaagacaaa tatgaaaaat tacttaaaga attaaaggca aagataaaag atggaactat
     3961 tacatcagtt ggtgttgaat ttgatggaaa accaattact acattagcac caaaggcaga
     4021 tggtagtgat aaagatgcaa tagcagaaca gttagaaact cttactaaaa accaacttaa
     4081 aggtttaggt gatggaaaat atgtagattt taaaattact tatggtgcta aagcagaagt
     4141 tccagctgct tcattaagtg cagatgatat acaaaaatat gcagatcaaa taaatgcttc
     4201 tgagaaaata ttagttgaag tagcagcagg atctgaagca ggtatagcaa aatttgattc
     4261 tgttaacaat aaagttatag caggagatgc tcctcttaaa gttaaagatg cagtgaaagc
     4321 gattgttact actaatggta gtaataaaaa agttcttaca atatcagctg cagctggatt
     4381 atcaggattt tcatatggaa cacttaaaga cacaggtgca agttcatctg atgttgatgc
     4441 aataactctt gacactacaa atgcaacaat aacagaaggt gatactaaag tattagattt
     4501 tgataattca tttaagttta acgaaagtac taaaaaagtt ggtagtttag ttacaccaaa
     4561 tacaacaaac actccagcag atcctggtac taaaactact gttagagtga taaaagctgt
     4621 tgaaaaaaca atagatgtaa gttcaaattc aactacaaaa gctaaagatt tagctaaaca
     4681 atatgtattc acagatgtta gtgatactga tccagaaagt ttaagttata tgcttaaaaa
     4741 cataaatgat ggaaaagttg ctgttaaaaa ttctgatgga gattatgaag taactatatt
     4801 cccagaagga aaaagattaa atactttatc tgcttcttca gctaaaacta ttttaggaga
     4861 taaagatact ccagcaaaaa tagttttaaa ggcttcaaca acaaagaaat tagcagacta
     4921 tatagatgat ttaataactt ataacaatag ttattcaaat gtacaaactg tagcaggtag
     4981 tgatagaata gagactgcta tagagctaag ccgtaagtac tataactcta ctgacaaaaa
     5041 tgctttatat ggagatcctg taaataacgt tgtattagta ggttctcaag ctatagttga
     5101 tggattagta gcatctcctt tagcagcaga aaaagatgct cctttattgt taagttctaa
     5161 agataaatta gattcatcaa caagagctga gataaaaaga gttatggact taaattcttc
     5221 aacaggaata aaaaataata aagaagtttt catagcaggt ggagtaaatt ctatatctaa
     5281 agacgttgaa aatgaactaa aagatatggg acttaaagta actagattat caggtgatga
     5341 tagatatgaa acttcattag cgatagctga tgaaatagat ataaatgata aagcttatgt
     5401 ggtaggtgga acaggattag cagatgctat gagtattgct ccagttgcat ctcaaataaa
     5461 agatggtgaa gctactccaa tagtagttgt agatggaaaa tctgacaagt tatctaaaga
     5521 agctgaagat ttcttagatg atgcacaagt agatataatc ggtggagaaa atagtgtttc
     5581 tgctaaaatg gaagattata tagatgatgc tactggaaaa tctccagaaa gaattagtgg
     5641 tgctgataga caagcaacta atgctgaagt tataaaagaa tactttgata aagatggagt
     5701 ttcaaattac ttcttagcaa aagatggttc aactaaagag gatcagttag ttgatgcatt
     5761 agcagcagca gcagtagctg gaaactacgg ttctaagcat aatgaagatg gagatataac
     5821 tacagatgct tctcctgctc caattatttt agcaactgat aatttatcag ctgaacaaca
     5881 tgtagctgta agtaaaactg ctactactaa tggtgctaag aacttagtac aagtaggtca
     5941 aggaatagct gattcagttg ttagcaaatt aaaagattta ttagatatgt aatataaatt
     6001 ttatatattg aataaaaaag gtttctcttt tgagaaacct tttttatttt aaacataaat
     6061 ttttataaat aaaaagaggc taattagcct ctttttaatg tgagaaacac ttaaaaaata
     6121 agatgtaatt atttatttaa gtatcattat tatactatac cttaaaataa atgtctacta
     6181 ttctataatt ttaaggtata atagtaagtg gaaaataaat aaggaggtaa attaaatatg
     6241 tcagttttag atgtaatatt agacaaaaca gatgaattag aaattaaaaa attaaataat
     6301 atagtagata aaatagatgc tttagaagat aaaatacaaa tactagatga tgaagcatta
     6361 aaaaatatga cgaatgtatt taaggataag ttaagtaaag gagaaacctt agatgacata
     6421 ttgccagaag catttgctgt tgtaagagaa gtatcaaaaa gaatattagg tatgcgtcaa
     6481 tatagagttc aattaattgg tgggattgta ttacatcaag ggaaaatagc agagatgaga
     6541 actggagaag gaaagactct agtaggagta gctccagttt acttgaatgc attgactggt
     6601 aagggggtac atgtcatcac ggttaatgat tacttggctc aacgtgataa agaacttatg
     6661 caaccagtat atgaattttt aggtttaaca gtaggagtaa ttattaatgg gcaacagcca
     6721 gtagaacgaa aattacaata tcaatgtgat attacttatg gtacaaacaa tgagtatgga
     6781 ttcgattatt taagagataa tatggtaacg caaaaatcag agaaagttca aagagaacta
     6841 aactttgcta tagttgatga ggttgattca atacttatag atgaagctag aacaccactt
     6901 ataattgctg gtggaggaga tgaaggtact aagctgtatg aacttgcaaa tgcttttata
     6961 aagaatataa gagatgaaga ttttgaaatg gatagaaaag ataaaaccat agcacttact
     7021 gcaagtggtc ttagtaaagc tgagaaattt tttggaatag aaaatataac tcatatagat
     7081 aatatagaaa tttttcacca tgtaaatcaa tctctaagag cacataaatt aatgaacata
     7141 gatgttgaat atgttgttag agataatgag atactaattg tagatgaatt tacaggaaga
     7201 ataatggatg gaagaaggtt tactgatggg cttcatcaag caattgaagc taaagaggga
     7261 gtaaaaataa atgatgaatc aaagaccatg gcaacagtaa catttcaaaa tttctttaga
     7321 ttatactcta aattatcagg aatgacaggt acagcaaaga cagaggaaag tgagtttgaa
     7381 gctacatata agattaatgt tgttcagata ccaactaata aaccagttat aagagctgat
     7441 ttacatgata aagtattcaa aacagaacgt gcaaagtaca atgctgttgt agaagaaata
     7501 ataaggataa ataaaactaa gcaaccagtg cttgtaggaa ctgtttcaat tgaaaaatct
     7561 gagaaattat ctaagctact taaaaagaaa ggtgtaaagc acgaggtctt aaatgctaaa
     7621 caacatgata aggaagctga aataatatct aaagctggta aattaggttc tgttacaatt
     7681 gctactaata tggcaggtag aggaactgat atatcacttg gagctggaga taaagaagaa
     7741 gaagaaaagg tgaaagagtt aggtggcttg tatgttatag gtactgaaag acatgagaca
     7801 agaagaattg ataatcagct tagagggcgt tcgggaagac aaggtgaccc tggaacttct
     7861 agattctttg taagtttaga ggatgaagta ataaaattgt atggtggaaa aagtatagaa
     7921 aaaatattaa agagaatgaa tgctaatgaa gatgacgcgt tagaaagtaa ggctttgaat
     7981 agagcaatag aaagagcaca gaaaggcatt gaaggtaaaa attttgaaca aagaaagaat
     8041 gttcttaagt acgatgatac tattaatgag caaagaaaag taatatatga tgaaagaaat
     8101 aaggtattgg atggttctga tattgaagaa gagatacaaa aaatggttat ggatattatt
     8161 acggaggctg ctgaaaagta cttaaaaaaa tttagagact atcatggata ttttaagtat
     8221 ttatataata cttttatgcc agctgatact ttattaattc cagacttaga taaaaaaaat
     8281 gttgaagaaa tagtaaatca aacatatcag atttcaaagc gtgtttatga tcttaagaag
     8341 atgatgattg gaattgaaga attggctcaa ttagagaaac gagtattact tcaagttgtg
     8401 gatacttatt gggttgacca tatagatgca atggatcaac taagacaata tataggtctt
     8461 aaatcttatg cacaaaaaga tccatttaaa gagtatgcta tagaaggtta tgaaatgttt
     8521 gaagctttaa ataggaatat aagaataaca acagtgcaat atctatataa atttaactaa
     8581 aaaagtgacc cattgggtca ctttttatat attaagatta aagtgaaatt tattttacag
     8641 tacacactca tatagataaa tatttataat aatgaaaaag aggctgaacg tcagcctctt
     8701 ttcctgtgtg agaaatacgg aaaaaagaga tgcttgaaat ttctttcttc catttaatat
     8761 tatactataa agagaataaa aagtccatga gttatcttta tgattaaaaa aaaaaattac
     8821 aattttgtta ctttttagta tacaataata aatttaaatt gactttgtta atttttatta
     8881 ataaaatatc caaatattac aatatctaca gtttattgaa ataatgtgta aattatatta
     8941 taattggtaa ataaggaaaa atggtgaaaa ccaaattttg tagggggaaa tatatgaata
     9001 gaaaaaatct ttatgtaatt atggcagctg caatggtaag tacatcagta gctccaagtt
     9061 ttgctgcaga agctacacaa gtaaagaaac aaacaataac taaaaaggaa gcaacagaat
     9121 tagtttctaa agttagagaa ctaatggctc agagatatac aggaggttct caagttggac
     9181 aaccaatata tgaaataaaa gttggtgaaa cttcatcaca attaaaaata ataactaata
     9241 tagacgaatt agagaaatta gtaaatgctt taggagaaaa taaagagctt cttgtgacta
     9301 tagcagataa aggacataca acaaatagtg caaatgaagt ggttgctgaa gcaattgaaa
     9361 aatatgaaaa ttcagcagac ctttcagctg aagctaattc aataacagaa aaagctaaaa
     9421 ctgaagctac tgggatttat ggagtggcaa ctgtagaagc ttcatatgac agtacaaaag
     9481 ataagttagt aataacttta aaagataaga ctgatgtagt aacttctaaa acgctacaaa
     9541 taggtattgg tgatgaaaag gttgatttaa cagcaaatcc agttgatata gcaggaacca
     9601 atttagaccc ttctgcagaa ggatttagag taagtaaaat aaataaatta ggtgtagaag
     9661 gagctaaaaa tattgctgat attcaattag ctgaaataac tataaaaaat agtgatttaa
     9721 atacagtttc acctcaagat ttatatgatg gatatagatt gactattcga ggtaatacaa
     9781 tagcaaatag tgtaggaaaa tcaattagtg atatttcatc aaaagattca gagacaggaa
     9841 agtataaatt tactgttaaa tatactgata catctggaaa agcgatagaa cttactgtag
     9901 aaagtactaa tgataaagag ttaaaagatg ttaaagatgc cttaaatgga aatacaaaag
     9961 tcaatttaat agctggggaa gacaggtatg caactgctgt agctatagca aaagaaacag
    10021 aatatactga caatatagtt atagttaatt caaataaatt agttgatgga ttagcagcaa
    10081 caccactagc tcaatctaaa aaagctccta tattgttggc gtctgataat gaaataccaa
    10141 aagtaacttt agattatata aaggaaataa ttaaaaaaag tccatcagct aaaatatata
    10201 tagtaggtgg agaatcagca gtatcaaata cagctagaaa gcaattagaa tcagtaacta
    10261 agaatgttga aagattggct ggagatgata gacatacaac ttcagtagca gtagcaaaag
    10321 ctatgggttc ttttaaagaa gcatttgtag taggtgcaaa aggggaagct gatgctatga
    10381 gtatagctgc taaagctgct gaacttaagg ctcctataat agtaaatggt tggaatgatc
    10441 ttacagcaga tgctatcaag ttgatggatg gaaaagagat tggtatagtt ggtggttcta
    10501 ataatgtacc tagccaaatt gaaaatcaac ttgctgatat tgataaagat agaaaggttc
    10561 aaagagttga aggtgaaaca agacacgata ctaatgctaa agttatagaa acatattatg
    10621 gcaaattaga taaactatat atagcaaaag atggatatgg aaataatggt atgttagtag
    10681 atgcattggc agcaggtcct ctagcagcag gaaaaggacc aattctttta gctaaaaatg
    10741 atataacaaa ctcacaaaag aatactttag ataaaaaact aaatcttggt gcagaagtaa
    10801 ctcaaatagg taatggggtt gaattgactg tagttcaaaa aataggcaaa atactaggat
    10861 ggtaataatc aataatatag aaaaggattc cttattattt ataggaatct ttttctatcg
    10921 acataatatt cattaaagta taacatgctc ttgacagata ggaggatatg atagtgatag
    10981 aagcaacatt gtttttttcc ccacaccaag atgatgaaac attaagtatg ggtagtgcaa
    11041 taattgaaca tgtagaaaga agcgatacac atgttatatt atgtactgat ggcagtaaat
    11101 caataataag aaaagtatta gatgatggag ggtcatgttc ttatcatatt aaagatgtac
    11161 acaagtactc tttatcagaa agtgattttt caaaaaatag agatgaagaa tttaaaaata
    11221 gttgtaaagc aatgggagtt aaagaaaata atatacatat agaaaataat agagctcatg
    11281 atggagaatt gagtaaggaa aaagcaagag atataatgct aaaatattta gaaaaatatc
    11341 ctgatgctaa agtgaagacc gttacaccat ataaagctct tggaatacat gaagaccata
    11401 gagcattagg tgaagctgca ttagaattgt atagagaagg aaaaataaaa gatttaagat
    11461 tttatgtaga gccatacgat tataatgatt ttaaaaaagt taatccaaat gttagagtat
    11521 ggaaggtttt gagtagtcaa gaagagaaat tattaaatgc tatggatgca tacaagaaat
    11581 ggaatcctga aaatggtaac tacgcaattg gatatcattc tgtaaaatct cactttgatg
    11641 aattaaaatc caataaaatc caatatgtac atgctccata aacattaata taaaaactag
    11701 gaggatatag ttaatgaaaa tatctaagag aataatgtcg ttgttgacag caactttttt
    11761 tatctttgca ttatctatca atatagctga tgcttcatca aaagagagtc tagtgggctc
    11821 tggtagatgg gaaactgcaa taaaaattag tcagaatgga tggaaagatg ctactaatgc
    11881 tatactagta aatgataatt ctatagctga tgcattatct gcaactccat ttgctaagtt
    11941 aaaagatgca cctattttat taactcaaag tgataaattg gatagtagaa ctaaagcaga
    12001 attaaaaaga ctaggtgtaa aaaatgtata tttaattgga ggtatgaata ctctaagcac
    12061 caatattgaa aaacaattaa aatcagaaaa tatgactttt gagaggattt caggtaatag
    12121 tagatatgaa acatctttaa aattagctga aagattagat aaagagaaga gtatttctga
    12181 tatagttgta gttaatggag agaaagggct agcagatgca gtaagtgtgg gttctgctgc
    12241 agcacaagaa aatatgccaa ttatactttc agatcctaac aatggaataa aagttgcaaa
    12301 tagctttatt agtagtaaaa atataaagaa atcttatata attggtggta catattctgt
    12361 atctagttct attgaacaga gtttacctaa tgtaaaaaga ttatctggaa acaatagaaa
    12421 tgaaactaat gcaaaggttc tagaagaatt ttataaaaat acagacttaa aaaatgctta
    12481 tataactaaa gatggtatga aaaaacaggg ggatttgata gattctctag ctgtaggtgt
    12541 attggcagct aaaaatagct ctccagttgt attagtagga aattcacttg actctagcca
    12601 atatgatgtg ttaaatacta aaagttttga taatattgtg caagttggtg gaaatggaaa
    12661 tgaagatgct gtaaaggata ttcttgattt acaagaagaa actatatata gagttaagac
    12721 agcagacgaa ttggatgttg ctctaagaaa agcagatgct aatgatatta taacatttaa
    12781 acctgatgat aataaaaaaa ttactagttc atttagtata gaaacaaaga aagctataac
    12841 tgtagagctt gaaggattgt atacacaaac aataactgta aatatgccaa atggagaatt
    12901 aactaataag ggtacattag atggaactgt gaaaataatg gatataaaaa atggcacatt
    12961 tatcaatgaa ggtgatataa atgatattga catttatgat ccaaatggat gtaaaataga
    13021 gaataaaagt ggtggagata tatgggtttt aactatatct agtagtgcta aagatgtaaa
    13081 tatagaaaat aaaggtgata taagtaaggt tacaaataat tctactagcg ttataataag
    13141 aaattcagga aatattgata ctttaagtgg aattaatgca ccagcaataa gtggaaataa
    13201 acctagagtt gatgataccg ataaggatga aaaaagagca agtggaatat atcctacttc
    13261 agaaccttta tcacctgcaa gagctggtta tataagtgta aaaatattca ctaagcctga
    13321 agatacaaat tataaaattt attatagagt agtaagcagt agaccatctg cattatatat
    13381 cggagataga atagatacaa gtgactggac tgctgtacca tcttctgttt taccatttac
    13441 tattaaagca gaaaatggat attatataga agctgttgaa ataaattctg atactaagaa
    13501 agtatctaga tgggggaaat ctgactatat agatgatgga ttttatttaa aatcagaaac
    13561 tgcaaaagga ttaagtgcag atatagattt tgatggagat tatgttaaat taacagcaac
    13621 aggtgctgat tctggatgta cagtttatta tagaatagta tcaaaaaagc cagtatcaat
    13681 gaatgttgga gataaagtaa ctatatctga ttgggatgaa attactacta atagagtgag
    13741 gatagaagca aacgatgtaa aggataaata tatagaacta gttgaattgg atgattcaac
    13801 taataaagta actagatggg gagcaagtga taaggtagta gaataggtat ctaatgtgaa
    13861 acataaaaac agactgatta ataatgggca gtctgttttt atttcctaaa aattatcatt
    13921 ttactaaata aataatttaa aactataaca attatattag taaatacttt tacagtaata
    13981 tcatttacaa aaaataaatc aatcattata tacataataa tcatttctat aataccagaa
    14041 agtagcctaa aactgacaaa aaacaaaaac tccttaaaca aaaatattag ttcaattctt
    14101 ttactctcaa acacaaaaaa cttattagta acataagcaa ataaaacagc aagtatccat
    14161 gccaaagcat ttgcaaccat aaaattaaat aaaataactc tagtaaaaaa taaatacgaa
    14221 actatattta caagagtagt aaatgctcca aaaaataaat ataatatcgt ctctctatgc
    14281 ttttttaaaa tcaaatataa tcctcctaag ctaatttaat ataaatatac ttaaagatac
    14341 taacaaacat tagtataaca catctattaa taaatcaata tatcttaaaa tattataaaa
    14401 ataaaactat aatattctat aattaatatg tactcataat ccatatttta tcaaaataaa
    14461 atatgacctt aatttagaaa tctgatataa taaaaattaa atacatataa gggggtaaac
    14521 atgagaaagt acaaatcaaa aaacttgtca aagctactag cattgttgac agtttgcttt
    14581 ttaatagtgt caacaatacc tgtctcagca gaaaaccata aaactctaga tggagtagaa
    14641 actgcagagt attctgaaag ctatcttcaa tacctagaag atgtcaaaaa tggagacaca
    14701 gcaaaatata atggagtaat accttttcca catgaaatgg aaggtacaac acttcgaaat
    14761 aagggtagaa gtagtcttcc atcagcatat aaatcaagtg tagcatacaa cccaatgaat
    14821 cttggtctta ctacaccagc aaaaaatcaa ggaagtctta atacatgctg gtctttttca
    14881 ggtatgtcaa cgttagaagc atatcttaaa ttaaaaggat atggaacgta tgacttatca
    14941 gaagaacatt taagatggtg ggcaactggt ggaaaatatg gatggaactt agatgatatg
    15001 tcagggagtt caaatgtaac agctatagga tatctgacag catgggcagg tcctaagtta
    15061 gaaaaggaca taccatataa tcttaaatct gaggcacaag gtgcaactaa accttcaaat
    15121 atggatactg cacctactca atttaatgta acagatgttg ttcgtcttaa taaagataag
    15181 gaaactgtaa agaatgctat aatgcaatat ggttcagtga catcaggtta tgcacattat
    15241 tcaacatatt ttaataaaga tgaaacagca tataactgta caaataagag agctccatta
    15301 aatcacgctg tagcgatagt aggatgggat gataattatt caaaagataa ttttgcatct
    15361 gatgtaaaac cagaatcaaa tggagcatgg ttagtaaaaa gtagttgggg agagtttaat
    15421 tctatgaaag gattcttctg gatttcttat gaagataaaa ctctactaac agatacagat
    15481 aactatgcaa tgaaatcagt atcaaaacca gatagtgata aaaaaatgta ccaacttgaa
    15541 tatgctggtc ttagcaagat aatgtcaaat aaagtaacag cagcaaatgt atttgatttt
    15601 agcagagact ctgaaaaact tgattctgtt atgtttgaaa cagactctgt aggagcaaaa
    15661 tatgaagtat attatgcacc agtggtaaat ggagttcctc aaaacaattc aatgacaaaa
    15721 cttgcaagtg gaacagtatc atattctgga tacataaatg tacctactaa ttcttacagc
    15781 ttaccaaaag gtaaaggagc aatagtagta gttatagaca acacagcaaa tcctaataga
    15841 gaaaaatcaa ctttagcata tgaaactaat atagatgcat attatttata tgaggctaaa
    15901 gcaaacttag gtgaaagcta catacttcaa aacaataagt ttgaggatat aaatacatat
    15961 agtgaatttt ctccttgtaa ctttgttata aaagctataa caaaaacatc ttctggacaa
    16021 gctacttcag gagaatcttt aactggagca gatagatatg aaacagcagt taaagttagt
    16081 caaaaaggat ggacttcttc acaaaatgca gtattagtaa atggagatgc aatagtggat
    16141 gctttaacag ctacaccatt tacagcagca attgactctc caattctttt gacaggaaaa
    16201 gataacttag attcaaaaac taaggcagag ttacaaagat taggaactaa aaaagtttat
    16261 ctaataggtg gagaaaattc tttaagcaag aatgtacaaa ctcaacttag taatatgggt
    16321 atatcagtag aaagaatttc aggtagtgat agatacaaga ccagtatatc tctagctcaa
    16381 aagttaaata gtataaaatc tgtttcacaa gttgcagtgg caaatggtgt aaatggactt
    16441 gcagatgcaa taagtgttgg tgcagcagct gctgataaca atatgccaat aatacttact
    16501 aatgaaaaaa gtgagttgca aggtgctgat gagtttttaa attcatctaa aataactaag
    16561 tcttatataa ttggtggaac agctacttta tcatcaaatt tagaaagtaa gctttcaaat
    16621 ccaacaagac ttgcaggcag taatagaaat gaaactaatg ctaagataat agataaattc
    16681 taccctagct cagatttgaa atatgctttt gttgttaaag atggttcaaa gagtcaagga
    16741 gacttgatag atggactagc agtaggagca ttgggtgcta aaactgattc accagtagtt
    16801 ctagttggaa ataagttaga tgaaagtcaa aaaaatgtac ttaagtctaa gaaaatagaa
    16861 actcctatta gagttggtgg aaatggaaat gaaaatgctt ttaatgaact aaatactctt
    16921 ttaggaaaat agtgaatgga aagatagtaa ggatatacat tttattatta aaaaatgttc
    16981 aatagaagtt tataaatata aaataacaaa aaggggtgtc tcaaaatgaa ctttttagtt
    17041 catgaggcac tcttttttgt tgttttatat ttttatcatt taaataatga aaaataggat
    17101 tgtctatatt tttagatagt tttcttatta ttgatgaaaa atgtaataat tttaaactgt
    17161 aaaatagtgt aaagttattt tataatataa atatagtaag attttaaaca aaataagggg
    17221 gaataataat gaaagcacca aaaactattt taacaatact gacaatagcg cttactttaa
    17281 gtagcatttc tataatacca tcatatgcac ttacagagga aaaattaata ggtaatggta
    17341 gatatgaaac tgctgtaaag ataagtcaaa aggcatacag ttctagtcct aatgtagtgc
    17401 tagttaatga caattcatta gctgatgcac tttcagctac accatttgca aaggctaaag
    17461 gagcaccaat acttttgaca gaaagtgata agctagatga taggactgaa aaagagataa
    17521 aacgtctagg agctaaagat atttatttga taggaggtac tgctgtactt aataaagata
    17581 tagagaacaa gttaaaaagt aatggactta atgtagaaag aattaatggt aaggatagat
    17641 atgaaacttc actgattttg gctaataagc ttaaggatat aaaagatatt aaggaagttg
    17701 ctgtagtaaa tggtgaaaaa ggattgagtg atgctgttag cgttggggca ccagcagctc
    17761 aaaataagat gcctataatt ttgtctagtc ctaaggatgg agtagaagca tttgataaat
    17821 ttataaggga tgagaaagtt ataaaagcat atgtaattgg aggtacaaat tctgtttcaa
    17881 gagctgtaga aaagagtctt cctaatgcag aaagagtgag tgggaaagat agaaatgaga
    17941 ctaatgctaa agttatagaa aagttttaca ctgatacaaa tttaagtaat ttatatgtaa
    18001 ctaaggatgg tagtaaaaat gagaatcaac taatagactc gttagctgtt ggcgtattgg
    18061 ctgcaaaaaa tgagtctcct gttgttttgg ttggtaataa attaaacaca aaacaaagag
    18121 atatacttag tactaaaaaa ttaaatacta taacacaggt aggtggaaat ggaaatgaag
    18181 aagcttttga tgaaattaaa agtctacaag aaaaaactgt gtttgaagca aaaactgttg
    18241 aggaattaac tgatatgata aatatagcaa gtcctaatga tattataaac tttaaaccaa
    18301 aagagaatac tgtaaatgaa gcttttagaa tggtaacaaa taaaccaata actgtgaata
    18361 taaagggtga ttgttcaaag actcttacag tagatatgcc taatggtgaa gtgaataact
    18421 atgctacttt ggtaaatgtt atagttagaa atattggaga aggtggattt aataatcatg
    18481 atacaatcac tatattgtca gttcgtgaca aaaatggtag agttatagaa aatactagaa
    18541 attcagatat agatacgttg atgatattag cttctgctaa tgatacaaaa ttgattaatg
    18601 atggatatat aggaaaactt atagataatt catctaatag tgatataact aataatggta
    18661 ctatagataa aaaagttaat caggttgaag atttagaggc taaagtagat tcaattgaaa
    18721 aagctataga ttctataagt caaaaagtca ataaaataca agatatacta gataagcttg
    18781 gatttctaaa gaaatttctt agctaagaat atgtatattt aagtgaagtg tgtgttgtaa
    18841 ttgttctaaa gttatgtagt taaacaggtt tatatacaaa tagatttata gataaatatg
    18901 tagtttagta tatatttgaa taagctttgg ggaggtaaca tggggattgg aattgaatgt
    18961 aacaaaaaaa taaataaaag tattgacaaa actagaataa agaaaaataa tct
//
LOCUS       DAMJWS010000082        18839 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMJWS010000082 DAMJWS010000000
VERSION     DAMJWS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.083"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(292..684)
                     /locus_tag="PC399_002936"
     CDS             complement(292..684)
                     /locus_tag="PC399_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2593782.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            1251..1613
                     /locus_tag="PC399_002937"
     CDS             1251..1613
                     /locus_tag="PC399_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593783.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1597..2304
                     /locus_tag="PC399_002938"
     CDS             1597..2304
                     /locus_tag="PC399_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593784.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDISNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEIINQFKNESENIRNQEVVEFTEEDEEELKQNIIENIK
                     KNFDEKNLVQVYKYRGV"
     gene            2324..2743
                     /locus_tag="PC399_002939"
     CDS             2324..2743
                     /locus_tag="PC399_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593785.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEVYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2733..3257
                     /locus_tag="PC399_002940"
     CDS             2733..3257
                     /locus_tag="PC399_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593786.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKCSQKYE
                     TDFKIKVWNFSESI"
     gene            3569..4126
                     /gene="efp"
                     /locus_tag="PC399_002941"
     CDS             3569..4126
                     /gene="efp"
                     /locus_tag="PC399_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF2593787.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4218..4673
                     /locus_tag="PC399_002942"
     CDS             4218..4673
                     /locus_tag="PC399_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2593788.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4836..5546
                     /locus_tag="PC399_002943"
     CDS             4836..5546
                     /locus_tag="PC399_002943"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF2593789.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5600..6670
                     /locus_tag="PC399_002944"
     CDS             5600..6670
                     /locus_tag="PC399_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2593790.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6698..10252
                     /gene="smc"
                     /locus_tag="PC399_002945"
     CDS             6698..10252
                     /gene="smc"
                     /locus_tag="PC399_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF2593791.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSTNLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10279..11559
                     /gene="ftsY"
                     /locus_tag="PC399_002946"
     CDS             10279..11559
                     /gene="ftsY"
                     /locus_tag="PC399_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF2593792.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPVPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11662..12030
                     /locus_tag="PC399_002947"
     CDS             11662..12030
                     /locus_tag="PC399_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF2593793.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            12027..13379
                     /gene="ffh"
                     /locus_tag="PC399_002948"
     CDS             12027..13379
                     /gene="ffh"
                     /locus_tag="PC399_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF2593794.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13417..13689
                     /gene="rpsP"
                     /locus_tag="PC399_002949"
     CDS             13417..13689
                     /gene="rpsP"
                     /locus_tag="PC399_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF2593795.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13754..13981
                     /locus_tag="PC399_002950"
     CDS             13754..13981
                     /locus_tag="PC399_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF2593796.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14089..14604
                     /gene="rimM"
                     /locus_tag="PC399_002951"
     CDS             14089..14604
                     /gene="rimM"
                     /locus_tag="PC399_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF2593797.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14613..15308
                     /gene="trmD"
                     /locus_tag="PC399_002952"
     CDS             14613..15308
                     /gene="trmD"
                     /locus_tag="PC399_002952"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF2593798.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15493..15843
                     /gene="rplS"
                     /locus_tag="PC399_002953"
     CDS             15493..15843
                     /gene="rplS"
                     /locus_tag="PC399_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF2593799.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16164..17060
                     /gene="ylqF"
                     /locus_tag="PC399_002954"
     CDS             16164..17060
                     /gene="ylqF"
                     /locus_tag="PC399_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF2593800.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17449..18741
                     /gene="brnQ"
                     /locus_tag="PC399_002955"
     CDS             17449..18741
                     /gene="brnQ"
                     /locus_tag="PC399_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2593801.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7963 a         1760 c         3466 g         5650 t
ORIGIN      
        1 ctataaaatg acaaaataaa aaatactatt taaatagata ttaaaaaatt gataccaagt
       61 atttgtttgc tatatgaata acaatttatt taggagcttt acttggacac tgtataataa
      121 taaagtaatt gctaaaaacg tttgatgatt tggatgtatt tttagaaatt agaaaaatat
      181 atacttactg actaatgata atcttatatg tattttagtt tgagtgattt aagaaaagtg
      241 ttttgaatgg ggattatttt atagagtaaa taaatatata atagtagaaa attacatata
      301 aagagtagta tttatttcat taccttcttt atctgttccg atagggtctt gtagagatac
      361 ttgagtttta ttttttttgt tagatctaat attcattaaa atctcatttt caatacatct
      421 agaagcatat gttgctaatc gagttccttt atctatgtta taagtttcta tagctttaat
      481 taatcctatt gtacctatgg atattaaatc atcctgatct tccgttgaat tattatattt
      541 ctttgctata taagctacta atctcatgtt tctttcaatt aatgtatctt ttgcactttt
      601 atcgttttct attttaaatt tagttaaata ctcaatctct tcttcagggg ttaagggttt
      661 ttcaaaagat ttaagagctg ccaataggtc accccctttt caacggtcac tttaaattat
      721 atgaaggaga cttatccaag ttgactgtta tctaataaaa taatatgcat atttttagaa
      781 tttaaaacat atatttgcaa acttttgaat ggaatatgac ataaagagtg ggaatttaaa
      841 tttaagtttt tgttgtatat atatgaaaca aggcgaagaa attattgatt tataaagtat
      901 aaattggaat tgaggtgtca ataaatataa aaggaaatag tatttatata tgttttggtg
      961 ttgaagtaaa agattaatgc aaagcattag agtctataaa aatatgaata taggaggtgt
     1021 aattttacaa tacttaaaaa agtaaatgta attaattgtt tgtataaagt tgattacaag
     1081 caaaagtatc agtcatagtt aatactgtta aggatgaagg tggtgtgtta atttatatat
     1141 gtatgggaag aaattttgat tacaatgaaa aaacttacaa aagatattta cgaaaaaact
     1201 tatagttaaa tgtttatata taaaatatta tttaggggtt gtttttatta ttgaaaataa
     1261 gaaagagtgg ttttatatca attgaatgta taataagtat tgctatatta tatgtggctg
     1321 tttatttagt ttctacatca ttgtataatt gttatagttt tatcagtaga aatatatctg
     1381 acagagaaat gttaagtaca gcaaaaaaat atatagaaga tgagaagtat agaatacaaa
     1441 atagtaagta tgagttaatt gaagataaga tagaaaaaaa ttacataaat ggatatgaaa
     1501 ttaacagtag aatagagcaa attttagatt attatcaatg ctatgaaata aatatagaga
     1561 taaaaaatga atttaaaaaa ctgaggttta atagctatgt tactagaaaa taaaggctct
     1621 atattaataa taacattgat aatattttct attataagta ctgtgtgcat tatgtgtata
     1681 ggtttaattt atacacataa taatatgttt gagttaggat acaaagacat tcaactaaaa
     1741 gaaatttctt taagtggaat agaaatatca gtaagtaatg tactttcttg tgttgaggaa
     1801 gctattgata tatctaataa tgaagacgaa tttagagaat atttcttagg aaataatatg
     1861 attgaatgta taaaaaaaat aaaagataca tcttatagta gtttagaaga actttcttta
     1921 aaagtagaaa ataatactat ttatgacaga ggagattttt ttgaatttga tatagaaagt
     1981 aatgttagag aaaaagaatt tttaaaagca ataaaagtaa gagtgaaaat caaaaatcca
     2041 tggataggaa ttgatattga agattatatg gataaatcta ataacaataa aattgatgac
     2101 aaaacattca ataattataa agcatctgaa aataatgaaa ttataaatca atttaaaaat
     2161 gaaagtgaaa atataaggaa tcaagaggtt gttgaattta cagaagaaga tgaagaagaa
     2221 cttaaacaaa acataattga aaatataaag aagaattttg atgaaaaaaa cctagtacag
     2281 gtatataagt ataggggtgt ataactttga aaaaagaagg aatgtgactg taatagaatt
     2341 aatagtatct atcagtataa tactcttgat agttacatta tcttttccaa aagataatct
     2401 tgaaaatcat aaaataaatt tatttgcacg tcaactttgt tcagatataa ggtatgttag
     2461 gcgaattaat atgtttagca actacaatac atatatatat tatataaatc aaggtaaaca
     2521 cgaaggttat gtattgagaa aagatggtaa aaacattaaa tcaataatgc tacctaaaaa
     2581 tgcaaaaata gctaggagta ttgaaataat aaagtttaaa tctgatgggt cgcctcaaaa
     2641 ggcgtctaca atagaggtat ataataatag attaagaaaa actataacaa taacacctgt
     2701 aagtggaaga gtacttctga aagagggaaa atatgaaaca taaatatggt tacttgcttt
     2761 tggaaagtgt cgtatcattg tcttcaatgg tgataattat actagtttta tattctatat
     2821 ttttgtctac aataaactta aagttaaaag tagaagataa gatagaacta caacaacaaa
     2881 gtcttgaaat cataaaatct atggaaggaa ttattagcaa ttctatggga ataatgaatg
     2941 tatctaatta tgaagaaaca tttaaaaaga caacatctat aaaatgtaga tatttagatg
     3001 aaaataataa tgaagaaggc ataagtaata aagaaattat attgaatgaa agacgaaata
     3061 agttatttgt aaattcttta aatggcgaaa gtagtcaagc aggtggttat gaaattggag
     3121 attatgtaga tgaaatgtat gtatcaatta ctaataatgg tcaatacgtt aatattaaat
     3181 tgaaattatc aaaatgtagt caaaaatatg agactgattt caaaataaaa gtatggaatt
     3241 ttagtgaaag tatataaaaa tacataaatt taagagaaag aaatgacatc aataagtcat
     3301 ataaaatata attattgaga actatataac taaataatat caatatgtaa agtgtataga
     3361 ttaagaatat agaggataat aataaaaata tagaataagt aatttgccaa atacttgttt
     3421 atattgtata acttatgata ttatgatatt agtaaaaatt tgtaatcctg acttgacaga
     3481 attcaagtaa gagatataat tttaaaggca tagtaatgta aaatttacta aaaaataaga
     3541 aatttatttt aatatggagg aacaaaaaat ggtatcagca ggtgatttta gaaaaggtgt
     3601 tacatttgaa aaagatggac aaccatgttt agtagttgat tttcaacacg ttaagccagg
     3661 taaaggagct gctttcgtta gaactaaata caaaaactta aaaacaggag ctataagaga
     3721 agaaagtttc aatccaagtg aaaaattccc taaagctgtt atagatacaa gacaaatgca
     3781 atatctatac aatgatggtg agttatatta ttttatggat caagaaaatt ttgaacaaat
     3841 accattaaac tatgagcaag ttgaagatgc aattaagttc ttaaaagaaa atgaagttgc
     3901 tacaataaga ttttaccaag gacaaccatt ccaagtggaa gcgccaaact ttgcagagct
     3961 agaagttaca gatacagagc caggcataaa aggtgataca gcaagtaatg taactaaagc
     4021 agctacagtt gaaactggag cagttgttca agttccatta tttataaata ctggagataa
     4081 agtaaagata gatactagaa caggtgaata tttatcaaga gtataaaaat ttatgtatat
     4141 aaaagcttat atgtcttaat aatataagat atatgagctt ttagtttata tataaaaaag
     4201 aaagcgaggg gtgttacatg aaacatttat atgaagaagt tgcatactta aaaggcctag
     4261 cagaaggact agaaataagt gctgaaagta aagaagggaa aatgatacat aaaatagttg
     4321 atgcattaga agtatttgca gatgctattg taacattgga tgaagaacaa gaagaacttc
     4381 aagattttgt tgaatctata gatgaagatt tagcagattt agaagaagat attgctgaca
     4441 tggaagaaga tctttatgaa gaagatgacg atgaggatga tgaggacttc agctatatag
     4501 aaatggaatg cccaaattgt ggagagttag tagaaataga cgaagatttg ttatatgatg
     4561 atgaagtaga tgttgtttgt cctgattgta aggctgtgat attatcatca gaagatgatt
     4621 gtgatgatga ttgtacatgt ggtggttgtt caagttgtga tgatagagag taaaaattca
     4681 taagaattaa aactgcctat gatgcctatg agatattttg cttcataggt agttttattt
     4741 ttatgaggaa gggtattatg tattttggtt ttgcaatact tggacaaaaa tagtacacta
     4801 gaatttatag aataggattt tggagggatg taaatatgaa aataagtaaa aaattactag
     4861 ataatataca aagatttgaa aatgttataa attataaatt taaaaataaa gaatatatac
     4921 tagaagctct tactcatagt tcatattcta atgaaaataa aaaatataat tttaacgaaa
     4981 gattggaatt tttaggggat tcggttttag gcatagtgat aagtgattat ttatttaatg
     5041 aagaagctaa tctgcccgaa ggggaactaa ctaaacttag agcaaatata gtctgtgaag
     5101 attcattaag tgaagtagct aatgatataa atttaggtat tcacatgtta ttaggtagag
     5161 gagaagaagc aacaggagga agacatagga cttcaatatt agcagacgca tttgaagctg
     5221 taatagctgc tatatatctt gatggaggtt ttgaaagtgc aagacagttt atacttcatc
     5281 atatggaaaa tataatttat gattcaagaa aaggaaatat atttagagac tacaaaacgc
     5341 atcttcaaga ggtgttacaa gggaatggag aaaataatat ctggtacagg cttatagaag
     5401 aaaaaggacc agaccataat aaaagatttg taatggaagt tggaattaat gacgatgtac
     5461 ttggtatagg agaaggcaag agtaaaaaag aagctgaaca gttagctgct aaaatagctt
     5521 taaagaaaaa attatgggaa aaataaatat aatatgtaaa aatataaaaa gaaatattta
     5581 tattagaagg atgttgtaga tgaaaaagag aattatacct atatttgttc ctcacaaagg
     5641 atgtcctcat gattgcatat tttgtaatca aaaaaaaata acaggtgtga gtactgatgt
     5701 tactagtgaa gatgcaagaa atataattga ggaatgttta gagactatag ataaagatgc
     5761 agatgtagaa atagcatttt ttggtggtag ctttactgct atagatattg atatacaaaa
     5821 aagtttatta tcagttgcta aagaatatgt agaaaaaggt cttgtaaaag atattagaat
     5881 gtctacaaga ccagattgta ttgatgaaga tatattaagt atgttaaaag aatataagac
     5941 aagtataatt gaactaggag tacaatcctt agatgaaaaa gtattattag atagtataag
     6001 aggtcatcaa tctgaaattg tatacaagag ttcaaagatg ataaaaaata gtggaatcaa
     6061 acttggatta cagatgatga ttggtcttcc agctgacaca gaagaaaagt gtatatttac
     6121 agcaaaaaaa tttgtagagc taaaaccaga ttgtgttaga gtctatccaa ctttggtagt
     6181 aaaagataca ggtcttgaaa agttaatgga acaaaataaa tataatccat ttacccttga
     6241 agagagtgta aatatagtaa aaaaagttct tgtactgttt tatgttaata atatcaatgt
     6301 aataagagtt gggcttcaag ctactgatga tatacaaatt ggaaaagctg ttttagcagg
     6361 accatatcat cctgcattta gagagttggt agaagcagat atgataaaag attatttaga
     6421 atttgttatt ttgcaaaata aaaatataaa gcaaatgttg gtcaaatcta ataaaaagaa
     6481 tatttctaaa atcataggaa ataaaaaaac aaatgttaaa tatatggaag aaaaatttgg
     6541 tgtgctttta aaaacacaag aatcagattt agatataaat caattagaaa ttgtgttaga
     6601 tgggaaaagt ttaattagtg ctaatatgag agacattcat agaaaattgt atgacattta
     6661 tgacctttag ttttaataat gaagggagag aaaagatttg tatttaaaaa gactagagtt
     6721 aaagggattt aaatcctttc cagtaaaaac agatattatt tttaaagaag gaataacagc
     6781 catagtagga ccaaatggga gtggaaaaag taatatatct gatgctgtaa gatgggtatt
     6841 aggagaacag agtattaaga gtcttagagg tgataaactc gaagatgtaa tatttgcagg
     6901 tacagataca aaaaaaccta tgaattattg tgaagtggct cttactattg ataattctga
     6961 aaatcaattg gaattagatt ttacagaagt aactataaga agaagggctt atagaaatgg
     7021 tgaaagtgaa ttttttctaa ataataaaag ttgtagatta aaagatataa aagaagtctt
     7081 tttagatact ggtattggaa aagatggtta ttcaatcata gaacaaggaa aagtagatga
     7141 aatattaagt aataatcctt taagcagaag aaaagttttt gatgaagctt gtggcatatc
     7201 aaaatataga tataaaaaac aggaagcaga aaggaattta agtaatacaa aggaaaattt
     7261 agaaagaata gatgatgtat atatagaaat tgaaaatcaa ttaaagcctc tttttaatca
     7321 acagacaaag gcaaaaaagt acttggaaat aagtgaaaaa ttaaagacac ttgaagtaaa
     7381 tagctttata agagaaattg aaggaataga aaaagaatta agtgaggtta atgaacatcg
     7441 taaggttatt gaaaaggagc tcaatgaaaa agaagaacaa aaaaatgttg tagagaaaaa
     7501 gcaagaagat attaataaag aggtagaggt attacaagat gtaattgaaa aatctgtaga
     7561 ttatataaat tcaattaaag gagttatatc aaaaaaagag tcccaaataa atttaattaa
     7621 ggaaagaata agaaatttta cgaatgaaat tagtagaaaa aatttagaaa taaatgatat
     7681 aaaagagaag ctaaatgaaa ataagcaata cataaaagaa ttagaaagca ataaattatc
     7741 aggtagtgaa gaactatcta ccttgcagga aaatataaaa gtattggaag caagtaaaga
     7801 taaacaaaaa attaagttag aatccctaaa taatgagata gaattattga aagaaagtat
     7861 aattgatata ttaaataaaa aacaagagtt tagtaataag ctgtccactt taaacgcaaa
     7921 taaagaaaat atgaatatca gagatgaaaa tataaattca gagataacag agttaaacaa
     7981 aaatattgaa ataaaatcat ctgaattaga tactataaat aaagaattta atatgcaaaa
     8041 tgaaaattta aagaatgtaa ataatagaca taaagaatta tcaactaatt tgcaggattc
     8101 aattagtgaa cacaataaac tagaagatga aatacagaaa agtaaatata atttaaatgg
     8161 atataattca aagttaaatg tctatataga tatggaaaat cattatgaag gatttaatag
     8221 aggagtaaaa gaagtactaa aaaataaaaa tttaaaagga gttcatggag cattaggaca
     8281 gattataaat gtacctgaaa aatatgagaa atctatagaa gcagctcttg gtgcttatat
     8341 gcaaaatata ataactgata atgagtttag tgctaagtct gcaataaact atctaaaaca
     8401 gaataattta ggtagagtaa catttcttcc attaaatata attaaatcaa ataaaataag
     8461 tctaggaaat ttaaaagcaa atactaagtt cataggaata gcaagtgatt taattacatt
     8521 tgatgaaaag tatagaaata taattgaaaa tatacttgga agaacaatac ttataaataa
     8581 tatagatgag ggcataaaat ttgcaaaaga aactggtcat aggtttaaaa tagtaacttt
     8641 agatggtgaa atcttaaatc caggaggttc tttgactggg ggaagtctaa aaactaatgg
     8701 gaatatacta tccagaaaaa gatacattaa tgaatataca gaaaagataa gtaatatcaa
     8761 gaatgaaatt tcacatctag aattaaaaag agaatcttta gacaaagatg tgaaaaatat
     8821 caaaaatgaa attgattcac atgaaagtaa aataaaagat ttagaaaaaa gtataataat
     8881 aaaaagtaca agtataaaga atgttgagtc tgaaattgaa tcgttaaaag gtagtattac
     8941 taaattagaa aatgagaaga atgatttaaa ttctaaccta aattatacac ttgaaaaaag
     9001 tgatgatgtt agaaaagata tggaagagtt agatgattta tacaataaaa ataaagaaaa
     9061 aattgatgca ttgaatgagg aaataaaaag atacaatgat ttatatgata aagaaaaatc
     9121 tgaatttgat gaattgaact taagtttagt gaagaaaaca gaagtttaca atagtatagt
     9181 tagagatata aaaagaatat ctggtgaaaa ttgtgagcta gaagaaaaaa ataaacaatt
     9241 agaagagtca ttaaattatg aagaacatga aataattaaa ttacaagatt ctatactcac
     9301 tgaagaaaaa gaaaaagaaa atttaacaaa acaactaggg gatagtaata gaaatcttga
     9361 aactagaaaa atagcaaaag atgatttaaa aaatagtttt gatgaaatta ataaagaatt
     9421 aaaaactata gatagacagc atatagaact taaagaaagt ttatttaaag ttggtggaag
     9481 attagaaaga ttaaaaacta gtcaagacac atatataaat aagttgtttg agcaatatga
     9541 tatgacctta gtacaagcat tggaaattaa agatgaagat ttggatatag atagaaaatt
     9601 cttagaaagt ctaaagagag aaataagaag tcttggaaat ataaatatag actctataaa
     9661 ggaatatgag gaaataaaag aaagatacga tttttatagc gaacaaaagc aagatttaga
     9721 agagtctatg gaagagatag aaaaacttat acatacttta gaagaaaata tgaaatctga
     9781 atttgaaatt aagtttgaag aaataagtaa aaattttaaa tatgtatata agagattatt
     9841 tggtggaggt tgtggagaat taactatttt agataaagaa aatttattag aaagtgatat
     9901 actaataaca gctcaacccc caggtaaaaa aatgaaaaat ttaaacctat tatctggtgg
     9961 agagaaagct ttaacggcaa taagtatatt atttgctata ttgattacaa agccaacacc
    10021 attttgtata ttagatgaga ttgaagcgcc acttgatgac gcaaatatat ttagatttgg
    10081 agaatttttg aaggatttat ctaaagagac tcaatttata tcagtaactc ataggagagg
    10141 aactatggaa gctgctgatt atatctacgg agttacaatg caagaaaaag ccatttcaaa
    10201 agttattagt ttaaaattaa aagaagctca ggaaataaca gatattatat aacttagatt
    10261 gacataggag gaataacagt gttaaaaaaa ttatttggtt ttggaaaaga caaggaaaag
    10321 gaaatagaaa aaaaagatgc tgaagaagaa atagaagttg aagacagtgt tgataattta
    10381 gaaaacttgg aagaaactat tttttcagga cttgaagaag aagtgataga taaagtggaa
    10441 gatgttgaag aaaaaaatga ggaaagtgtc aataatgaag gcactgaaga aatcgaaaac
    10501 tttgaaaaaa tagaagttga gatggatagt aatggaaaag aagtagaaaa tatttcaaat
    10561 aatgattctc aagaagagat aatcgaagaa ttagaaaatt atgatgaaat tgagtctaaa
    10621 gagatagaag ataaagaaga taagaaagtt aacttgtttg aaagactaaa acaaggattg
    10681 acaaaagcaa agcaaggaat aactgataga atagatgaag ttttaaaatc atatacaaag
    10741 atagatgaag aattacttga agatttagag gaaattttaa taacagcaga tgttggtgta
    10801 aatacaacta tggatataat tgaaagacta agagataaaa taaagcaaaa aggtattact
    10861 gaaccaataa aggtaagaga agaacttaaa tctatagttg aagatattct tacaaatgaa
    10921 aactctacac tagatataga accagtacca tgtataatat taatggtggg tgtaaatggg
    10981 gtcggtaaga ctactacaat aggtaaacta gcaaatagat acaaaaaaga tggtaaaaaa
    11041 gttttattag cagcagcaga tacatttaga gcagcagcaa cagagcaatt agaaatttgg
    11101 gcaaatagaa ccaatgtaga cataataaaa catcaagagg gagcagaccc tggagctgta
    11161 gtatttgatg ctataaaagc tgcaaaagct agaaaaacag atgtattaat atgtgataca
    11221 gcaggaagat tacacaataa ggctaatctt atgaatgaac ttggaaaagt atttaaaata
    11281 gtagatagag aatttccaga agcaaaacga gaagtacttc ttgtggtgga tgctacaaca
    11341 ggtcaaaatg cagttgttca agcaaagaca tttaaagaag ttgcagatat aacaggtata
    11401 gtacttacaa aacttgatgg aactgcaaaa ggtggagtag tccttgcggt aaaatcagaa
    11461 gtagatgtac ctgtaaaact tattggagtt ggagaaagtg tagaagacct acaagatttc
    11521 aatgcaaaat cattttcaga tgcattattt ggtaactaat gttcttttaa aaataatggt
    11581 tgacaagagt gttgttatga tataaaatac aatagtgtaa agtaaataac ctttacagta
    11641 tactgctagg aagtgtaaga tatgaatata gaaaaaatgg tcgaaattgg attgttattt
    11701 gaacagtata aagaattatt aactgacaag cagaaagaaa tagttgctct atactatgaa
    11761 gaagattatt ctttaggtga aattagtgaa aatctgaatg tatcaagaca aggtgtttat
    11821 gataccttaa agcgttcaga aaaaatacta agagattatg aagaaaagtt acacttagta
    11881 tcaaagattc aagagcagga aaaaaatatt aagattataa aagataaaat tattgacatt
    11941 aaagaagatt tattgcacaa tagagattgt gctaatttaa tccctaagct agaaaatata
    12001 gaagatgtat gtagggagat gataaaatga tatttgaagg attgtctgat aaactacaag
    12061 gggcatttag taagttaaaa tctaaaggaa aacttacaga agtagatgta aaaaatgcta
    12121 tgagagaagt taaacttgct cttcttgagg cagatgttaa ctttaaagta gttaaagatt
    12181 tcataaaaaa agttcaagag agatgtgttg ggcaagaagt tatgcaaagc ttaactccag
    12241 ctcagcatgt aatcaaaatt gtaaatgaag agcttacaag tttaatggga gatgtacaaa
    12301 gtaaagtaat gatttcctca aagcctccta caattttaat gatggttgga ttgcaaggtg
    12361 caggtaagac tactacagca ggaaaacttg gaggatattt taaaaagcaa ggtaaaaaac
    12421 ctctattagt agcttgtgat atttatagac cagcagctat aaagcaactt caagttgttg
    12481 gagaaaaatt ggatatacct gtattcaata tgggagataa agaaaatcca gtaaatattg
    12541 cgaaagcagg tttaagccat gcgattaaaa atgctaatga tttagttatt atagatacag
    12601 ctggtagact tcatatagat gaagtactta tggatgagtt aaagtctatt aaatcagaag
    12661 ttaagccaca tgagatactt ttggttgtag attctatgac aggtcaagat gctgtaaatg
    12721 ttgcagaaag cttcaatgag gcacttggtg ttgatggagt agtattaact aaattggatg
    12781 gagatactag aggaggtgct gcactttcaa taagagcagt tacacaaaag cctataaaat
    12841 ttatggggat gggagaaaag ctagatgata tagaaccttt ccacccagat agaatggcat
    12901 caagaatatt aggtatggga gatgtcttaa gtttaataga aaaagcacaa gaaaatatag
    12961 atttagagaa agctaaagaa ttagagcaaa aaattaaaaa acaagagctt gattttgaag
    13021 atttcttaca acaaatggaa caaatacaaa atatgggacc tcttgacaag atacttggta
    13081 tgatacctgg tatgggaaat gtaaaagacc aacttggaga tgtagatttg aatggcaaag
    13141 aaatgaagag aacaaaagcc ataattcagt caatgactac atatgagaga agaaacccag
    13201 gagtattaaa tgcttcaaga aagaaaagaa tagctaaagg cagtggaact agtgtacaag
    13261 atgtaaatag acttataaaa caacttaatg aaatgaaaaa aatgatgaag atgtttgctg
    13321 gttcacaaaa gagtatgaag aaaaggggcg gcttaggcgg tttacctttt tttaaataaa
    13381 atataaaaaa gtaaaaatta ttcggaggtg actgtaatgg cagttaaaat aagattaaaa
    13441 agaatgggag ctaacaaaaa acctttctac agaatagtag tagctgattc aagagctcca
    13501 agagatggaa aatttataga agaaatagga tactataatc caatttctga acctaaacaa
    13561 gtaagaataa atgatgaaaa agctataaaa tggttagcta ctggtgctca gccaacagaa
    13621 gtagttaaaa aattacttgt aaaaaacgga gtaatagaaa aattcgaagc ttctaaacaa
    13681 gcaaaataat tttttcttgg caattagttt aagtaagggc tcattgctaa ggttacttta
    13741 ggagatgaaa tatatgaaag agctagttgt agatatagct aaggctctag ttgataatcc
    13801 tgactcagtt gttgttgagg aatttgaaga taatgatggt atcgtcttaa agcttacagt
    13861 tgctcaagag gatatgggta aggttattgg gaagcaggga agaatagcta aagctataag
    13921 aactgtcgta agatctgttg caaatagaga aaatataaaa gtttctcttg agatagtata
    13981 aaataggagg attaggtata atgcctagtc ctttttatat aaatgcgtag tttaagattt
    14041 tatgatgata tgaccacata agattaaatt ttttaagaag gtgataacat gaaagaaaaa
    14101 ttaactcatt ttaaggtagg tcaaatagta aatacacaag gtttaaaagg tgaagtaaga
    14161 gtttatccgc ttactgatga tatagagagg tttgatgaat taaaagattt ctatctggga
    14221 aaagatttaa ataataaatt ggctgttgaa aaagtaagat acaaaggttc tatggttata
    14281 atgaagataa aaggcataga cagcatagag aaagcagaaa aactaaaaaa taaatttatg
    14341 tatgtatcta gagaagatag cagagagctt gaagaaggcg aattttttat agcagatatg
    14401 attggaatgg aagttttaac tgtagatgga aaatatgtag gaatactaga agatgttctt
    14461 caatactcag caaatgatgt ctatgtagta aaaggtgagg aagataaaga atttatgata
    14521 ccagctataa aaaaatttgt acctacaata gatattgatg aaagaaaaat gattatagac
    14581 cctattaagg gaatgataga ttaggtggta atatgagatt tcatataatg acactttttc
    14641 cagaaatatt taattcgtac atggatgaaa gtataatgaa gagagcagtt gaaaaaggta
    14701 taattgaggt tcatatatat aatataaggg atttttctaa taataaacat aaaaaagtag
    14761 atgattatcc atttggagga ggagcgggga tggtaatgac tcctcaacct atatatgaca
    14821 catataagca tataataaca actcataata taaataaccc aagtgtaatt tatcttactc
    14881 caaaaggtaa ggtatacaat caatctatgg caaagcagat gtctttaaaa gaagatataa
    14941 tactactatg tggtcattat gaaggaatag atgagagaat cattgattta atagttactg
    15001 atgaaatttc aattggagat tatgtattaa ctgggggaga gctcccagca cttataatga
    15061 tagattctat atcgagatta ataccaggtg tattaaacca agaagaatca tttgaagaag
    15121 aatcatttaa agacaactta ttggaatatc ctcattacac tagacctaga gattttgaag
    15181 gattaaaagt accagaagtg cttttatcag ggaatcataa gaagatagat gagtggagaa
    15241 gagaagaatc tataaggata accaaagaaa gacgatttga tttatataaa aagtctaatg
    15301 aaaaataaaa atataatgaa aaattaatat atatgtatac aataaagtac ctataaatgg
    15361 aattaaaaac ataaaagtgc ttgtaataac tattgaagta tagtataata taaaatgttg
    15421 aaaatacggg cgttcctctg tttacactgg tcgggtagtt aagaacgtct atgatatcta
    15481 ggaggaagaa agatgaatga aatgctaaga gcaattgagc aagaacaatt aaaaaatgaa
    15541 gttcctaatt ttggacctgg ggacacagtt aaagtacacg ttagaattat agaaggaaaa
    15601 agagaaagaa tacaagtatt cgaaggtgta gtattaaaga gacaaggtgg aggagcaaga
    15661 gaaactttca cagttagaaa aatgtctttc aatgttggtg tagaaagaac tttcccagtt
    15721 cactctccaa aaatagaaaa aatagaagtt actagaaaag gtaaagtaag aagagctaaa
    15781 ctaaactact taagaggtag agtaggtaaa gctgctaaga taaaagaagc tagaaataaa
    15841 taattaaaaa ctttacttat aaatgggggg ctgatttatc agtcctcttt ctattttaat
    15901 gaaaattaca acaaataatt atactagaaa taaatagaaa tatttgttga aaatatagat
    15961 tatttatcta tgaatttata gcttttaaaa tgaaaaataa aattggtaaa tattgcatta
    16021 tattttgtaa cgatattaaa aataagagaa cagtattatg taaagtatga cgttaaaaag
    16081 atgacaatat tatagtttgt gaagatatat tttttaagtg gaattaggat ataataatat
    16141 aatattaata aggagtgata catgtgtcaa atgaatacga agagtatctt atggatgaca
    16201 atctacatat aaactggtat ccaggtcata tgaaaaaaac taaggagtta gtaaaaaata
    16261 acttaaaatt gattgatgta gtaattgaac ttttggatgc tagaatcccg tttagtagta
    16321 aaaatccaga catagacagg cttgtaggag ataagccaag agttgtcgta cttaataaga
    16381 gtgatatggc agatagggat aaattgaatc aatggattga atactataag aaaattaata
    16441 tcaaagctat accagtagat acaataaaag gtgttggtat aaataagata atcgaagaat
    16501 gtaaaaatgt aactagagaa aaaatgagtt ctttaaaaga caaagggaga aaagaaagag
    16561 ctatacgaat aatgatagtt ggcgttccaa atgttggtaa atcgtctctt ataaacaaat
    16621 taacaggtag aaaaagcact caaactggag ataagccagg agtaacgaaa gggaaacaat
    16681 gggttagatt aaagggaaat cttgaacttt tagatacgcc tggaatactt tggcctaagt
    16741 ttgaagacca agaagttgca ttaaatttag catttagtag agctataaag gatgagattt
    16801 tagatactga aactttggct ttaagactta tagaaaaact tatgaaaata gaacctgaga
    16861 agttgaaagc cagatataag ttagattgtc taggagaaac tccaattgaa actatggata
    16921 tgattggtca taaaagagga tttataacag gtaaaaaaga actagattat acacgcatag
    16981 ccacaactgt attaaatgaa tttagagatg gaaaaatagg aaatataaca ttggaagttc
    17041 cagaaaacgt taaaagatag attttaaaac aaaatcaaaa atttaatgtt aaaaatttaa
    17101 aaaagccttt tagcaaaggc ttttttattt ttttgaaata tagacaaata tctccatata
    17161 aaagtataaa ttaggttaga aagcctttaa aaatattaat ttaagacaaa tatacctaat
    17221 attttgaaaa ttaaaatgag tgtgattatt aaaaatttta gcatattgac ttgcttgttt
    17281 cgtataatat ataataagca caaataaata caatatatta aaaatttaat ttatttaatt
    17341 ttattaattt aataataagt tcttattgtt taattcaact tacttatgca agcgtaaaca
    17401 atcgagacat tttattaatt taataaggtt aaaaaggggg attttataat gggtaagacc
    17461 aaggatgtta tagtatttgg atttgcgtta tttgcaatgt tctttggagc aggtaactta
    17521 atatttccac cttacttggg aataattaca ggtccagaat ggttaattgc cttcttagga
    17581 tttacgtttg ctgatgctgg attggcactt ctagcagtaa tggctacagc taaattcgat
    17641 ggaaacgtag ttgaaatgtt taaaagatgt ggtatgaagt tagggattct tataggttgt
    17701 gcagatattt tatgtatagg accattctta gctataccaa gaacaggtgc aacaacttat
    17761 gaaatgggta taatgccttt atttggaact tcaattccag tgttattatt ctgtatactt
    17821 ttttttgcgc tttcatatgt actaacaata agaccttcta aggtcgtaga tatagttgga
    17881 cagtttttaa ctccagctct tttaatagct ttagcattta taattataaa aggtattata
    17941 tcacctcttg gagatatagt agataaacct atgataccta atgtatttgc agaaggtata
    18001 ggtcaaggat atcagacaat ggatgctttt gcagcaatag cacttgcatc agttctaata
    18061 gtatctttaa atgataaagg atattctact attagtgaca agttaaaaat gattggtaag
    18121 gcgggtgttc ttgcttgtgg aggattagct ctagtttatg gaggattatg tttccttgga
    18181 gcaactgtat caacaatgta tggaacagat gcagtacaat cacaagtaat agtaaatata
    18241 actgaaggat tgcttggaaa tgttgggaaa gcgattttag cagtagtagt atcattagca
    18301 tgtttaacta catctatagg tcttacatct gccacaggtc aatatttctc tagacttaca
    18361 aaaggtaagt tgagttatga aaaaatagta ttagctgtat ctgtattcag tgcagtagta
    18421 gcatcttttg gtgttggaac tataataaag atagcatctc ctatattaag tatagtttat
    18481 ccaccatcaa ttgttttaat aattttagct ttcttcaatg aaaaaattaa gaatgataat
    18541 gtgtacaaag gagcagtata tatgtcattg cttgtaagta tactaacagt tatatctagt
    18601 tatggagtag ctgttccagt agtaaactct ttacctctta attcattagg atttaactgg
    18661 gtggtgcctg ttataatagc tggaataatt ggcaatttta taccatcaaa gagtcaatct
    18721 aatacacttg gaacaaacta agttttattt aattaaaata taatcaattg taaataaatg
    18781 agttaactaa aagtaaaagg agttatttgt acaaataact ccttttattt ttagattct
//
LOCUS       DAMJWS010000083        18607 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMJWS010000083 DAMJWS010000000
VERSION     DAMJWS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.084"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            158..1318
                     /locus_tag="PC399_002956"
     CDS             158..1318
                     /locus_tag="PC399_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2593802.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1661..1822
                     /locus_tag="PC399_002957"
     CDS             1661..1822
                     /locus_tag="PC399_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593803.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2015..2644
                     /locus_tag="PC399_002958"
     CDS             2015..2644
                     /locus_tag="PC399_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF2593804.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMGGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2884..4143
                     /locus_tag="PC399_002959"
     CDS             2884..4143
                     /locus_tag="PC399_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2593805.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4402..5397
                     /locus_tag="PC399_002960"
     CDS             4402..5397
                     /locus_tag="PC399_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF2593806.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5643..6710
                     /locus_tag="PC399_002961"
     CDS             5643..6710
                     /locus_tag="PC399_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF2593807.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYNKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7244..9154
                     /locus_tag="PC399_002962"
     CDS             7244..9154
                     /locus_tag="PC399_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2593808.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9217..9840)
                     /gene="ytaF"
                     /locus_tag="PC399_002963"
     CDS             complement(9217..9840)
                     /gene="ytaF"
                     /locus_tag="PC399_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF2593809.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10028..10672
                     /locus_tag="PC399_002964"
     CDS             10028..10672
                     /locus_tag="PC399_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2593810.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10886..11944
                     /locus_tag="PC399_002965"
     CDS             10886..11944
                     /locus_tag="PC399_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF2593811.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12048..13232
                     /locus_tag="PC399_002966"
     CDS             12048..13232
                     /locus_tag="PC399_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2593812.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13439..14152
                     /gene="rpsB"
                     /locus_tag="PC399_002967"
     CDS             13439..14152
                     /gene="rpsB"
                     /locus_tag="PC399_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF2593813.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14231..15142
                     /locus_tag="PC399_002968"
     CDS             14231..15142
                     /locus_tag="PC399_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF2593814.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15227..15931
                     /locus_tag="PC399_002969"
     CDS             15227..15931
                     /locus_tag="PC399_002969"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF2593815.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            15957..16514
                     /gene="frr"
                     /locus_tag="PC399_002970"
     CDS             15957..16514
                     /gene="frr"
                     /locus_tag="PC399_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF2593816.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16517..16720
                     /locus_tag="PC399_002971"
     CDS             16517..16720
                     /locus_tag="PC399_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593817.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16749..17483
                     /locus_tag="PC399_002972"
     CDS             16749..17483
                     /locus_tag="PC399_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF2593818.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17496..18275
                     /locus_tag="PC399_002973"
     CDS             17496..18275
                     /locus_tag="PC399_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF2593819.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7643 a         1857 c         3225 g         5882 t
ORIGIN      
        1 aaatctttat taatatatgt caatagttag gtatatatgt atttaattag catgatatag
       61 ataaaagtat ttattttaat tgtctattat ctgaaaaagt ctttacttag aaatcataaa
      121 aatcaaatta attaggaaaa gaaagggtgt gtataatgtg attgagactg gatttgtata
      181 ttgtagtttc ttggttgtat tcgtaggtgt aatttttttt atggttgata aattgaaatg
      241 gaaggtattt gatgttatta atccaatgct tttaatatac ttaggttcag ctatacttgg
      301 atcatttggt ttatttgcac agaatgaaga tgttggaacg tatcaaaaca taataacagt
      361 gaacttcctt ccattaatgt tagtgttttt gttaattcaa tgtgatttaa gaaaaatatt
      421 aaagatgggg cctaaaatgt tattatcatt tttctgtgct acatttacat ttatgcttgg
      481 atttgttata gcattcataa tttttaaaaa ttcaatgggt gcagattcat ggaaaattct
      541 tggggcagca tctggttcat ggataggtgg ctcatcaaat atgattgcag cagctaatgc
      601 tctaggagtt gcagaagaag gacttagtta tgcaatactt atgggttcta taggatatac
      661 agtatggatg tctgtttgtc taatgtctgt gaaatttgga cctaaattta acaaatggac
      721 aaagtgcgat acaagtttta tagatgaggt tacatcaaaa cttgaaaaag aaaataaaga
      781 taatacagca cctacgtttg tagaacttat gattttactt tctgttgggt ttggagttgc
      841 gtcttttgca caaattgtat ccaagatact tccaacttca ggtatactta atgaaactat
      901 gtgggtagta cttgttgcat ctacagtatc aatattattg ggtatgacaa aaatatcaaa
      961 gcttaaaggg agtacactgg taggaaatgt gtttatgtat ttactccttg cagctacagg
     1021 tagtaaagca aatttctttg gattgtcaga agcacctgtt tacataatgt ttggattggt
     1081 agtagtcctt gtacatgcta tttcatttat agggcttgct aaactattta aacttgattt
     1141 atttacttgt gaagtaggaa gtatagctaa tataggagga gtaactacag ctccaatgat
     1201 tgctggggtt cacaatcctg tattaatacc agtaggtgta cttatggggc ttctaggaaa
     1261 tattataggg acttattgtg ctcttatggt aactcaaata ttacatatgt tagcttaatg
     1321 aaaatatggt ataaaattaa taatttaaat aagaaaagtc atttcaattt aagcagaagc
     1381 ttttactgaa atggcttttt ttgtttgaca agataatcta tttcaagaag atgttttgac
     1441 atatttagtg tataatattg taaaatttac taaaaacgag atatatttaa tttttatatt
     1501 gaatcgaacg tctgtatgat gtataataaa tatacagaac gaatgtttgc ggaacgggtg
     1561 ttttttgaat ttattttaat aatttatgaa tatatatcag aatgcataaa tatatttaaa
     1621 tataaagatt aaaaagaatt taaaggagtg ttgataactt atgataattc catcaagaag
     1681 aatacaagat ataacattaa caaatttaaa aaatggtgaa gtaactttaa ttgaattgga
     1741 tgaaatatac aagaagatgg gatttttatt tgttgttagt gaaggcaaat tgaaaaagat
     1801 aaaaaaggag aatcatcatt agaaatgaat gaaataataa atattataat agtttataaa
     1861 acctaactta tgtagaatat aaatacttaa gttaggtttt tttgtgataa agtttataag
     1921 tactcaattt tataagataa ataatggttt taagtgaaaa gtaaataagt ttaaatttta
     1981 gtaagtatta tattgcataa gaggtgatat tattatgaaa atagtaacct acaatattca
     2041 taaaggtatg gattcaaata atagattaac tctaactaaa atggggttat atttaaaaca
     2101 attggattgt gatgtaattt gtttgcaaga agtattatat cctcaatttt tagctttaaa
     2161 agctgtttta aatatgggtg gtgtctttgc tacaaatgta aaaaaagtaa atatgatata
     2221 tggtatttgt acatttacaa catctaaaat gttaaacagt aatcattttt tcttaactag
     2281 caaaaaagaa caacggggag ctctttgtat aactatagat gcatatggaa gaattataaa
     2341 tgtaataaat actcacttgg gacttgatag gcaggagaga gcaaaacaat tagacgaaat
     2401 aatagactat aggaacagat tagtggggat agttatttta tgtggtgatt ttaatgagaa
     2461 aaatgtcttt ttaagtatgt ttaatgatat ggctatttca ctaaacaagt cttacttacc
     2521 aacatttgaa aaatcaaact ctagaataga ttacattttt gttaataaaa atactgaact
     2581 aaaaggatac acagtagaaa aaatttacct gtcagaccat tatcctgtaa taggttacat
     2641 ttaaatttca atataaatga aattagttgt tataaaataa acaatttttt tgttaagaaa
     2701 tagtaaaaga aatataataa aaaatatcaa aaataaaact aattataaga cataaattta
     2761 caataaagtt acatttgatt aaattccttg tttaacaaaa gctattcaaa tataatctaa
     2821 ggtagttttg aaatgggaac tctattatgc aaatttaagc tagtgttaaa atgaggtgaa
     2881 gttatgggca gaagaacaac aagagtttca agattaggta aagaaaaaaa acgaagtgtt
     2941 ttaattgtat tggcagcttt agttctaata atgggtggat ttacttattt ttttaacagt
     3001 aaatttctgt acaatgggaa aatcgctaaa aatgtatata tagaaggagt aaatgtatct
     3061 gatatgacta aagcagaggc attaaaagct ataacagata agtatactcc agaagactta
     3121 aatcttgctt atgatgggaa aaaatataca atatcaccaa aagatattga tttaaaatat
     3181 gatacagaag atgttgtaaa agatgcttat gaaagtacaa aaaaagggtc ctattttcaa
     3241 aacttaaaaa aatatattga tataagagtg aataaggcaa atatgaagat aaaagctgaa
     3301 tatgatgagg caaaacttag ttccaaagtg tcttctatag cagatagtat aaatgttaag
     3361 atgaaaaatg cttctattag tgttggaagt ggtggactaa gttatacaga ctctgttgta
     3421 ggtagagaat ttgatttggc agcaaataaa gagtctatat ataatatgat aaaaaataaa
     3481 gagcataaaa ctttagaact taaagtaaat cttcaaaaac cagatataac aactgaacaa
     3541 gtaaaaactg taaattctgt tataggtcaa tattcaacta cttattcaca agctgtagag
     3601 ggtaggtcat ataatgtagg attatcagca agaaaaacta gtgatgtatt attaatgcca
     3661 ggtgaagaat tttcatacaa taaacttaca ggacctagta ataaagcaaa tggttacaaa
     3721 gatgcacctg ttatagttta tggtaagcta gaacaatcag caggaggagg tgtttgtcaa
     3781 acatcttcaa cagtttataa tgctgctctt ctttcgggta tggagataac tcaagttaca
     3841 aatcattcat ctgcatctac ttatgtgcct aaagggagag atgctactgt gagcgatgga
     3901 ggattaaact taaagtttaa aaatccatat aagcatcctg tatatattaa aaattatgca
     3961 ggtggaggaa gtgtatcatc agtaatctat ggtaattctg gtgacaaacc aaatatatct
     4021 atagaggtaa aacaaactgg tcagaataaa tattctacat atcgaatatt taaagattca
     4081 agtggaaagg tcataaaaaa agaacatata tcaaatagtt cttataaaga attaaaaaaa
     4141 tagataattt tttagagaaa tatttgtaat ataaaaggga gtgtctcaaa atgaactttt
     4201 tagtttatga ggcactcttt ttctgtgaaa tcaaatatat tttcatcatt tcaacaatac
     4261 aaaagaagcg tatctctatt ttgagacagc cttttttgta aaaattaaca ctattagttc
     4321 ttgttttaat attacaatca ttaatatata gaaaatattc ctatatattg gtataattgt
     4381 aaatagattt agttatataa aatgtatgag gaggtgacat ggttgaattt tataggcaat
     4441 agatatgagg tagtaaattc tgatgacgtt ttagaaatta acaaaatcta taaagctaga
     4501 gatgtttttt acaggaaaaa tgtgttaata aaagttataa agcacaataa ttatatctgt
     4561 gaagattttg tttcaaattt aattgatgaa agtactgctt tagatgagat aaactctcct
     4621 tatatactaa aaataataga tgttgggatt cactgtacag aagaaacaac attatattat
     4681 gttgtaagtg aagactttaa tggtatcgga ctagatgaat taattctagg taattatctt
     4741 catttagaag caattgtaaa cataatgata caagttttaa aagctttaga aatgatacat
     4801 aggaatcatt catatcatgg tagtttaaaa tcgagtagta taatagtaga tacagaatac
     4861 aatataaaaa tttgtgattt tggaattaca aaagcaaata atggggttaa tacaagaagc
     4921 tatggaaaca ggagatattt atgcccacat caactatgta taaattatac tgataaagaa
     4981 agtgattttt ttgcaacagg tcttattcta tttgaatcta tatttaaaaa attgcctttt
     5041 ggagaatcaa attcagaaga aaaaatgcta caatctattg ataagggatt agattggaat
     5101 agtataaaag caattaatgg aaatactgaa cttataaatg ttataaaaag gcttcttgga
     5161 agaaataata agtatactag agccaatgat attattattg atttaagtaa agtaatgtat
     5221 gaaaaagcat atatagaagt tgaagatgag aaagaagaag agcagaaaca agacaaagtt
     5281 atacaagtta aaaaagaata taaaagtaaa atgagaaaaa aattacataa aaaacttgtt
     5341 gttgccggtc tagctatagt catgatttca atgattatta ttagttccat aatataaaaa
     5401 ttgtatatta agtattttaa aatcaatgtc tataaaggat atacatagaa attttagtgt
     5461 agtcttaaat tatgaaagga agtggttatt atgttaacaa aaaataatga ggtattaaac
     5521 gtctaggagt attttaacaa atagccacct gcttttacag tttagaataa aaaagctgtg
     5581 gctaaaggtc acagcttttt gctacttttt aaaggcagga gaatattaaa aaggagcaaa
     5641 ttatgagcaa tataaaaata atagcaagta ataaatgttg gattgaagat atagcaagga
     5701 aacaacttga agatatttca aagttaaatg gaattttaag agtagtagga ttacctgact
     5761 tacatgcagg taaaatacct gtaggtttag cagttgagac taaaaatatt atttatccac
     5821 atataattgg aaatgatata ggatgcggaa tgactttatt taaaactggg gttctaaaaa
     5881 agaaatttaa aaaagacaaa tggattaagt cacttagtaa gattaaagat ttgtccgata
     5941 tagaaataaa aaacacatat aaagaagaat gtccaatttt aaatttagga actataggga
     6001 gtggaaatca ttttgtagaa attcaatgta taagtgaaat ttataataaa gaacaatttg
     6061 aacaattaaa attttcatct gatgatatta tgatgttagt tcactgtggg tcaagaaatt
     6121 atggagaaga tattttaaaa aaattttatg ataaagatgg acttgaagtt gaaagtgaga
     6181 aagctataga ttatattaaa aaacatgata atgctttaat gtgggcagag cgtaatcgtg
     6241 aagctatcag taaaaaaatt atgcagtcaa taggtacatc tggagatgtt gaaacaatat
     6301 tctctataaa tcataatttt atagagaaaa gggaagataa atttatacat agaaaaggtg
     6361 ctgtttcaag tgaaagagga gctgtgatta taccaggttc aagaggcagt ctatcatata
     6421 tagttatgcc tactgaaaat acagagatat ctttatattc tctatcacat ggagcaggta
     6481 gaaaatggtc tcgttcagtt tgtaaatctc gccttaaaag taagtataat aaagatacta
     6541 taaaacaaac taaatttaaa agtcagatta tatgtaatga tgtaaattta ttatttcaag
     6601 aagctccaga ggcatataag aatatagagc agattattga aagtttgata gaatatgaac
     6661 ttattcaagt agtggcaact atgaagccat tgattactta taaaggataa tttttaaagt
     6721 taaaaaagat taaaattata ttataaatag ctaattttat ggaacattta aaagtgtttt
     6781 attattagct atttttttat tttgtagaga cgataattta tagagctaaa ttctttgata
     6841 tataatgatt acaaagttag actgtattgg aaaatgcaca tacatgaatt gatgacaata
     6901 taagtgattc ccaaatataa atgaaagtat aagcttattt tcactataaa aattttcact
     6961 gtacaaaaac attaatgaca ttatttttat tgacataaat atataattta tggaataata
     7021 ttagaagcac tgatataagt gtttaaataa gaatagaaaa ggagaagtgt tttatgaaaa
     7081 aaaatactaa cattaatgag tccggagtta aacttcttaa taaataagga gtttgatata
     7141 taataactcc ttatttatta agttgttcgc aaaataacta ttatcaaagt aaatgatagg
     7201 gtgatttaaa agacgataac ttataaataa aggagaaaaa ataatgaata gtaatacatt
     7261 tgaaagatta caactaaatg aagttaaaga attaataaaa atacattgtg taagttcact
     7321 aggaaaaaat ttaatagata aattaactcc tagtgggaat attggagtag taagaaggaa
     7381 attagcagaa aataaagaag ctagaaagat aattgaaaat agtaatcaca taccacttga
     7441 aggattgttt aatgcaggtt ctacaataga taaaattgaa aaaggaatga taatagaacc
     7501 tgttgaactt gtaaatattg aagatttttt aagaggttgt aggaagatga aagcttttat
     7561 gttagaaaaa gaattttatt cacctacatt aagttcttat gccttaaata taacagaatg
     7621 taaaagtata gaagatgaga taaattactg tataaaatct aataaagttg attctaatgc
     7681 tagtaaagaa ttaaaaaaga taagacgaaa tatagaaatt acagaaggaa agataaaaga
     7741 tagattaaat aaatttataa catcaactgt aaataaaaag tatatacaag agtttataat
     7801 aagtaaaaga aatgatagat atgtaatacc aattaaatct tcttataaaa atgaagttaa
     7861 tggtactata ttagatacat catcaaaagg aaatacggta tttattgaac ctattagtgt
     7921 ttcaaattta agtacagagt taactatgtt aaaagctgat gaaactattg aagaatacaa
     7981 aatattatct tatttaacag aacttatatt tgagaagata agtcagataa aattaaatat
     8041 agagatactt tcagaatacg atatggtatt tgcaaaagct aagtatagtc aaaaaataaa
     8101 aggaattact ccaaaaataa ataataatgg ctatataaaa ataattaaag gtaaacatcc
     8161 acttctaaca ggagatgcag tacctttaga ttttgaaata ggcaaaaatt atagaagtct
     8221 tataataaca ggtcctaatg caggaggaaa aactgtaacc cttaagactg ttggtttact
     8281 aactttaatg gttcaatgtg gatttgatat ttctgcaaaa gaaggtagtg aatttagtgt
     8341 atttgaaaaa gtttttgtag atatagggga taatcaaagt attgaaaatg ccttaagtac
     8401 tttttcatca catataaaaa atattgcaga gattatgagt ttatcaaata attctactct
     8461 tgttctgttt gatgaaatag gaagtggaac tgaacctaat gaaggagctg gtttagcaat
     8521 atcattatta gaagagtttt acaaaatggg atgtataact atagcatcaa ctcattatgg
     8581 agagataaaa aagtttgcta cattgcatcc agaatttgaa aatgctggta tgatgtttga
     8641 taaagaaacg ttagaaccat tgtataagtt aactattgga aaatctgaag atagtaatgc
     8701 tctttttatt tctaaaaaaa tgggaataaa aaatagagtt ttagatagag ctaaagaata
     8761 tgtaattgat agaaactaca atttagattt agttaaaaga agtaagctac aatctaatat
     8821 tgaagataaa aatagttctg aagtcatttt aaactataca gattataatg taggggataa
     8881 ggtaaaatta ctagatgaag atgattttgg aattgtatat aagcctatgg acaagtttaa
     8941 taatgtagaa gtattgttta atgataattt tatttcagta aatataaaaa gattacaact
     9001 tagcataaaa gcatcagaat tgtatccaga agggtatgat ttagatgtat tatttactag
     9061 ttttaaagaa agaaaattag aaaaagatat tcaaagaggt tcaaaaaaag ctcttaaaaa
     9121 gattcagaaa gaaattagag agaatagaaa ataaatataa aaagcacaaa agaaagcaat
     9181 ctaaagagaa aagtttatct ttagattgct ttcttattaa catatcttca taaatccaat
     9241 gactaacaag attaaaccac ttaaccaaga taagtttaaa tttacgtttt taacaaacat
     9301 atttcctagt aaacaaccaa gtaatactgc aattatattt gatattaaag aaaatagaat
     9361 aacttgtata taattcacac ctacaaggcc actaccaaaa ccgatagcta atccatctaa
     9421 acttaaagca agtgcaagtg taaatgcttc agcagattta agcactttag atttacttgt
     9481 atctaagaca tcaggttcaa tacttacatt aaaatttaat ttaaggtcta acatattaaa
     9541 attataattc tttgagtgta tagatttttt cttaaaataa gatttaaaaa aactttcaat
     9601 tagtctagca cttcctaata aaaataaaat aaagaaacat attaacattg ttatattttg
     9661 aggtaaaaag tctctaacta ttgtacctgc aaaaagcgat attgctaaaa tagctgaaca
     9721 aactacgctg ataattatat ttgaatataa gggaacttta actttattta cgccatatcc
     9781 aaagcttgtc acaaaggcat ctatacaaag agaagtaact aataaaattg attcaatcac
     9841 agtagattaa catcctttct cttaaatttt gtacttatat tagttatagt attgtaaaaa
     9901 tggataaatt gttacagata atccaataaa ttgttgataa aattccaaca tcatacaaca
     9961 cctaaataac aaaagaatca tatcatataa taatataaaa ccaatcataa taaagcaggt
    10021 gaaaataatg gaacaacata gtaaggttgg gaatttcggt gatttgttga aagttttatt
    10081 agatgaaaaa gaattatcaa tgggagaact aagtaaaaaa agtggaattg ataaatctac
    10141 tatatctcgt atagctaata ataaacaaaa gccaaatata aatcatttag aaaagatggc
    10201 aatacattta aatattaacc tagaagaact gttgaaagct tcaggatatg aatttagaaa
    10261 cagcaataat caaatattta atgcagatag tgattttagt aactttgatg atattcttgg
    10321 atttgcaaat ttaatcaatg ataagaactt taatggaaat attgaaaaag agttaagtaa
    10381 gtgtaagtta tatgtccaaa cagatgaagg taaaaaatta ctttttgata attttaataa
    10441 aaaaatagat tctattgaaa aacaaggtca atttacagat agattaagac aaatgtatgc
    10501 tgatttttgt acagatggac ttacaataaa gaaatattta ttgattggaa gtatgctact
    10561 ttactttgtt atatcaacag atgtgattcc agacttcgtg ttcccaatag gatttatgga
    10621 tgatttagta gcattaaata tagttacaaa attacttaaa aatgataagt aagttttaga
    10681 atcatattta atatataaat aaatcaaagt atataaaatt tattattttt tatatgaaac
    10741 aaattaaaag cacaatgaag tagattaaaa atttaaaact aagaatctaa tatatatttt
    10801 gtaactattt aagtatataa aataaaataa tatccaaact aatacaaaat agtaataatt
    10861 gttttgtact ggaggatata tatttatgaa ttgtaaatgg atatctaaaa tagatgagag
    10921 aaaaaaatgt catagagaag cagattcttc tggatattgt atctttcata aagaaaataa
    10981 gtctgatgaa gagatacaat tgatgatgga tactcttcat aaagaagaga taagtgaatt
    11041 taatggattt gtttttgaaa atgaatttaa tgcggaagaa atattaactt acaattataa
    11101 aatactggat ttttctgaat ccatatttaa acaaaaagca aattttaaaa aatatatttt
    11161 taaaaagaat attattttta actatacaga atttagagat aaagttttat ttaatggttg
    11221 tgtattctta gaaaactgtg attttaatag aaccatattt agtaaacact atataaatga
    11281 ccgtatattt gaaaaagtga aatttaaagg acctgatttg gtagttaata aagtagaaaa
    11341 ttttccacga atggatggaa taatttttag catgtgtaca aaatttgtgt taaagaatgt
    11401 tgaatatgga aaaagtgagt atgagcatgg caagataaat tatagaatag ctagaaatca
    11461 agcaacaaaa attggagaat atgagatgat aggattttac tattataagg aaagaattta
    11521 tagtagtaaa ataatgaaac gttctaatta tcctaccttc agtgattatt tagttgaaaa
    11581 attttttgac caaatagcac gttacacaac aggttatgga gaaaagccat ggaatatatt
    11641 attagttatt atagcaataa taagtgtttt tgctttatta tacttatttg taggcataga
    11701 aagttcaaat agcacacttg tagctttaga tataaataat ataggtgatt actcactaag
    11761 tgagattttc aaaatgtata tggatttatg gtattttagc atggctacat ttagtacagt
    11821 aggatatgga gatatggttg ctacaagttt gattgggaaa gcattggcag gtatagaagt
    11881 attttttgga gttacaatag gagcaatttg ggcatctgtg attattaaaa gaatgataag
    11941 gtaatatttg tgtaaatatg gataggtatt aattactaga tataaattgg actaaatagg
    12001 gaaaatttaa atataaatat acttaataat ccaagaaggt gaaaaatttg aaaaatgcac
    12061 taaaaaaaat tatatcatta gccatgattc tagtttttat aataccaaat tattcatatg
    12121 cagatgataa atctgtagac aagtattcaa aaacatctat acttatagac caagaaacag
    12181 gtagagtttt atatagtaaa gaacctgata tgaaggtacc attagctagt ttaagtaaga
    12241 tgatgacttt tttacttgca atagaagcta ttgaaaataa agaagtaaaa gaaactgata
    12301 aggtaaaaat agacaaatct attgcatcag taaaagggtc aagttataaa cttaaagatg
    12361 gtgaagaagt gccacttata gagcttatga gaggtcttat gattgtatca ggaaatgatg
    12421 cggcaattgc tatagcaaaa catgtttgta aaacgaaaga agcatttgta gatagaatga
    12481 ataaaaaagc aaaagaaata ggaatgagta atacacattt tctaaatcca aatggtcttc
    12541 caatatacga tttaagcaac cccaaaaaac cagcaaaaga aaatatctct actgcaaaag
    12601 atatagctat tcttggaaaa tacatgtttg accattatga aaaacaagta acagctataa
    12661 ctgatatgga gacatataca aattcagata gaagttttga aaaaagcaat acaaatgcat
    12721 tattaagaat tataccagaa gtagatggta taaaaactgg atatactggc aatgctggat
    12781 attgcttatc attttctatg gttgtaaata aaaatgataa aaatgagaaa aaacgtagag
    12841 taataggggt tgtattaggt actaaccata aaaataaaag aacctctgct tcattagctc
    12901 tactgaaata tggaaaagaa aatttcaata tgaaaaaagt aattgataaa gatagcttta
    12961 ttggtaagaa atatataaaa ggaacgaaag agttagaggt tacattaaaa gcaaaagatg
    13021 aactttatac aatattaaaa gatgatgaaa gtataaagtc agaagttaag attaaaaata
    13081 tagagtaccc tgttaaaaaa ggtgatacta tgggtattat aaaatattat actaattcag
    13141 gtgacttact tggaagtgta gatatagtaa gtaataatag tatagataac gtatcattaa
    13201 aaacaaaatt aaaaatgaaa tttgcaaatt aaattcaaaa aatggtataa aatacacaaa
    13261 aaaatataaa aatagaaaat atggaaaaaa tattgttatt tcttcacctc tgtgatatac
    13321 tactagaggt gaaaaaataa aataaatcac acacagcatt gggatcctta aaaggtgcct
    13381 attacatggt tttttaggga gaagatcaag ctggaggtat aaaaccagga ggtaagatat
    13441 gtcagtaata tcaatgaaac aattattaga agcaggtgtt cactttggtc accaaacaag
    13501 aagatggaac cctaaaatgg ctaagtatat attcacagag agaaatggaa tatatataat
    13561 cgacttacaa aaaacagtta aaaaagttga agaagcttac aagttcacta aagaagtagc
    13621 tgaaacagga aagccaatct tattcgtagg aactaaaaaa caagctcaag atgcgataaa
    13681 agatgaagct gaaagatgtg gaatgtactt tgttaatgaa agatggttag gtggaatgtt
    13741 aacaaaccac aaaactataa aaacaagaat aaataaatta agagaattag aaaaaatgga
    13801 agaagaaggc gtatttaatg ttcttcctaa aaaagaagtt ataaaattaa gagctgaaaa
    13861 agaaaaacta gaaaaatact taggtggaat aaaagacatg cctgaattac caggtgcaat
    13921 gttcgtagtt gacccaagaa aagaaaacat agctattcaa gaagctcata gattaggtat
    13981 accagtagtt ggtatagttg atactaactg tgacccagaa caattagact ttgctatacc
    14041 aggaaatgat gacgctataa gagccgtaaa attaataact ggtgctatgg caacagctgt
    14101 aattgaaggt agacaaggtg ctgaggaaga agtagctgaa gaccaagaat aataatttga
    14161 caattaaatg gtaagggtcg cccttaccat ttataatatg gaatagaata agtaggagga
    14221 aacttaaaaa atggctaata taactgctca aatggtaaaa gagttaagag aaagtacagg
    14281 cgcaggaatg atggactgca agaaagcttt acaagaagct gaaggaaaca tggaaaaagc
    14341 agtagattta ttaagagaaa aaggattatc aaaagctgct aaaaaagctg gtagagttgc
    14401 tgctgaaggt ctagttgcaa tagaaatgaa tgatgataat acagttgctt ctatggtaga
    14461 agttaactca gagacagatt tcgttgctaa aaacgaagac ttcaaagtgt tcgttaaaga
    14521 tgctgcttgt atggcattag ctactgataa agaagatata gcttctttat taggtgaaac
    14581 tcataaagaa ggaataactt tacaagaagt tttaaataat agagttgcaa aaataggtga
    14641 gaaattagac tttagaagat ttgctaaagt tgtaactaat ggacaagttg ctggatacat
    14701 tcacggtggt ggaaaaatag gtgttcttgt tgaaatggaa acagaagcta gagatgctaa
    14761 agttttagaa ttaggtaaag atgtagctat gcaagttgct gctatgaatc ctaagtatgt
    14821 ttcaagagat gaagtagatg ctgaatacat agcacatgaa acagaagtat taactcaaca
    14881 agctttaaat gaaggaaaac cagctaatat agttgaaaaa atggttaaag gaagattaga
    14941 aaaagaatta aaagaggttt gtttattaga gcaaactttc gttaaaaatc cagatataac
    15001 tgttaaacaa ttagttgctg atgttgctaa ggcagtaggt tctgatataa aagttgttaa
    15061 agttgtgaga ttcgaagttg gtgaaggtat acaaaagaga gaagaaaact ttgcagaaga
    15121 agttgctaag caacttaagt aataaccaaa gagagaacac gcaggtgttc tcttttttaa
    15181 aaaatagaaa aaattggata tgaaggattg aaataggagg tttctgatgg ataaaccaat
    15241 gtacaaaagg gtattgttaa aattaagtgg agaagcatta gcaggagaaa gaggttttgg
    15301 tataaataat gatgtagtta atgatattgc tattgctata aaaaaaatac aagaaatagg
    15361 tgttgaagta gctgtagtag taggtggagg aaacttctgg agaggcagaa ccagtgaagg
    15421 aatggacaga actactgctg actatatagg aatgttagct acagttatga atgcaatggc
    15481 gctacaggat gcactagaaa atatagatgt tgctacaaga gttcaaactg ctatagacat
    15541 gagacaaatt gcagaaccat atataagaag aagagcagtt agacacttag aaaaggaaag
    15601 agttgttata tttggtgcag gaacaggaaa cccttacttt acaacagata caactgctgc
    15661 tttacgtgct gctgagatgg aagcagaagt tatattgtta gctaaaaatg ttgatgcagt
    15721 atatgacaag gatccaaaag tacatgcaga tgctaagaaa tttacagaat taagttacat
    15781 ggaagttata cagaaagaat taaaagtaat ggattctaca gctacatctt tatgtatgga
    15841 taataaaatt ccaataaagg tatttgagct tacaacagag aatataataa gagcagtaaa
    15901 aggcgaaaat ataggaacta ctgtaaaata atgaagaatg aaggaggagt aaaattatga
    15961 aactggaaat acataagcaa ttagaagaga aaatgaatgg gacaattgat gctttaaagt
    16021 ttgaatttgg aactataaga gcaggaagag ctaatgcaca aatgctagac aaaataagag
    16081 ttgattatta tggaactcca actccaataa atcaaatagg tgctatatct gtaccagaac
    16141 caagaatatt aatgatatct ccttgggata aatctgctat gcatgaaata gaaaaagcga
    16201 tagctaactc agacttagga ttaaatccat caaatgatgg tgaggttata agactttctg
    16261 tacctgcttt aacagaagaa agaagaaaag aattagctaa aaaagctagt aaagctgcag
    16321 aagaatttaa agttagaata agaaatgaaa gaagagacgc caatgagaaa attaaaaaga
    16381 tggaaaaagg cggagaatta actgaagatg aattaaaaaa agcacaagat gaagttcaaa
    16441 aaatgactga taaatttata aaagaaatag atacattatt atctaaaaaa gaaaaggaca
    16501 taatggaggt ataatattga aaaaatatta taacctttta ggtcttcatg tagatgaggt
    16561 aaaacaatat tttgatgagc aaaatataaa ttataccgta aaatctattg aaggtaaaaa
    16621 ggataaagaa aaacttgtag ttcctagagt tataaaaata tctgagatag gtgatagtgt
    16681 agaactgata atcacatact tttctgactc cttagtttaa ggagtcagaa caatattgga
    16741 ggcaaaatat gaataacaac attatatatg atatagactt aaataacata cctactcata
    16801 ttgctattat aatggatgga aatggaagat gggcaaaagc tagatttctt ccaagaactg
    16861 caggacataa ggctggagta gaaactataa gagatatagt aaaagaatgc tcaaaattag
    16921 gtgttaagca cttaacatta tatgcatttt caacagaaaa ctggaaaaga ccgaaacttg
    16981 aagttgacac acttatgaac ttattatcta catacttaag aaatgaaata gctgaacttc
    17041 accaaaacaa tgtaaaagta actgctatag gagatataag tgctttacca aaaacttgta
    17101 taagagaatt aaactcagct aaggaactga caaaaaacaa tacaggagtt aacttaaatt
    17161 tagcattaaa ctacggaagt agagcagata taaaaaatgc tcttatagat atagtaaaaa
    17221 attgtgaaag tggtaaaata gatataaaca atattgatga agatattata aaaaactatt
    17281 taagcacaaa gtctatacca gatccagact tggtaataag aacaagtggt gaacaaagat
    17341 tgagtaactt tttactttgg gaagttgctt attctgagtt ttactttact gatatacatt
    17401 ggcctgattt taaaaaagaa gaactacaga aggctatata cgtatatcaa agaagggata
    17461 gaagatttgg aggacttaag taaggagatt attttatgct tacaagaatt attgcttcac
    17521 tagctctggt tccattattt ctatttgttg tatatggagg tattcctcta tatatagctg
    17581 agacagcaat agtttatatt gcattgcatg agttttataa ggcatttaaa ataaaagatg
    17641 tacatccaat attcattata ggatatttat tttctattta tttagctgta aaaaatattt
    17701 ttaatttacc attagaatat acttatgcag taatttttat tttattttta gcaagtataa
    17761 tttatatgtt gatgggcaaa aacaatgtta tagatgtttc gattacgttt ttaggagttt
    17821 tttatatagg agtattttta gattttataa ttataactat aaatggattt gaaaaaggta
    17881 gtatatatgt atggcttata tttgtgatat cttttatgac agatatattt gcatatttta
    17941 gtggttattt acttggaaaa cataagctaa ttcctaaagt tagtcctaaa aagactatag
    18001 aaggagctat tggaggaata ataggaagta cactttgttg tattttattt ggatatttat
    18061 ttggcattga tttgcttcaa ttagcgataa taggaagtat aggaagtgta atagctcaat
    18121 taggagattt atttgcatct tctataaaga gatatgtagg gataaaggat tatggaaaga
    18181 taatacctgg tcatgggggt atattagata gatttgatag tgttatatta gttgcacctt
    18241 ttgtatatag tgcgattaaa ttttttatta gataaagatt ggttggattt aaaagagcaa
    18301 tctataattg ctagtaatga aaatagctat gtaactttta aatgcagaga tattctgcag
    18361 ttattagtta catggctatt ttcattgttc aaaaatttag aagaccttgg ggaattgtat
    18421 aagaataggt attactttat aatagtatac tatatttgta gtgagtatat tttttatact
    18481 ataactatca ttaaaaaata tttttttgtt taataatttc gatattttta gctatattat
    18541 atatttataa tatatatgta taaatgtaac taatttatta tccatttata aattttattt
    18601 ttaaata
//
LOCUS       DAMJWS010000084        18419 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMJWS010000084 DAMJWS010000000
VERSION     DAMJWS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.085"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(229..909)
                     /locus_tag="PC399_002974"
     CDS             complement(229..909)
                     /locus_tag="PC399_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593820.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(959..2974)
                     /locus_tag="PC399_002975"
     CDS             complement(959..2974)
                     /locus_tag="PC399_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2593821.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(3046..3723)
                     /locus_tag="PC399_002976"
     CDS             complement(3046..3723)
                     /locus_tag="PC399_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593822.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(4303..4386)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(4619..5461)
                     /locus_tag="PC399_002977"
     CDS             complement(4619..5461)
                     /locus_tag="PC399_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2593823.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSVDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(5713..7497)
                     /gene="pepF"
                     /locus_tag="PC399_002978"
     CDS             complement(5713..7497)
                     /gene="pepF"
                     /locus_tag="PC399_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2593824.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLVHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(7563..8228)
                     /locus_tag="PC399_002979"
     CDS             complement(7563..8228)
                     /locus_tag="PC399_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2593825.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(8379..8540)
                     /locus_tag="PC399_002980"
     CDS             complement(8379..8540)
                     /locus_tag="PC399_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF2593826.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(8618..9127)
                     /locus_tag="PC399_002981"
     CDS             complement(8618..9127)
                     /locus_tag="PC399_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF2593827.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            9333..9551
                     /locus_tag="PC399_002982"
     CDS             9333..9551
                     /locus_tag="PC399_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2593828.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            9569..11515
                     /gene="feoB"
                     /locus_tag="PC399_002983"
     CDS             9569..11515
                     /gene="feoB"
                     /locus_tag="PC399_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2593829.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(11694..12749)
                     /locus_tag="PC399_002984"
     CDS             complement(11694..12749)
                     /locus_tag="PC399_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2593830.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(12797..13597)
                     /locus_tag="PC399_002985"
     CDS             complement(12797..13597)
                     /locus_tag="PC399_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2593831.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(13590..14381)
                     /locus_tag="PC399_002986"
     CDS             complement(13590..14381)
                     /locus_tag="PC399_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2593832.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(14421..14843)
                     /locus_tag="PC399_002987"
     CDS             complement(14421..14843)
                     /locus_tag="PC399_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF2593833.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(14859..15338)
                     /locus_tag="PC399_002988"
     CDS             complement(14859..15338)
                     /locus_tag="PC399_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2593834.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(15470..18226)
                     /locus_tag="PC399_002989"
     CDS             complement(15470..18226)
                     /locus_tag="PC399_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2593835.1"
                     /translation="MRDEILSFVNECVLFNSFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGIAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         6029 a         3156 c         2026 g         7208 t
ORIGIN      
        1 tatatagatt tagatataca atttatccat cttttcaaat ataatctata aaataatagt
       61 tccattacgc atttttttat ataataaaac tgactgtaat ttaactggca aagtctagaa
      121 gaataggatt tatcaattaa attacagtca gctattttat aataatttag tttagatatc
      181 gttagtattt cacatttata acaattatta atgatattaa tatatttatt attccttatt
      241 aaaataatat cctgctcctc ttattgttat aattttctga ggatttttat aatctttctc
      301 tattttcttt cttaatccat tcactgcaac taccacacta tagttatcct ctaagtattc
      361 attcatccat acatgttcat atatctcttt agtagtcaaa gtcttttcag gattctctat
      421 aaaaaagttt aacaacttat tttctgttgg agtcaatttt attattttat cttctaactt
      481 tattactcca ctagttctat tatattcctc taattttgta ttattttcat tattatttac
      541 atatgagttc attcttctta aaatagcttt tacttttgcg tataatattt ctaaatttag
      601 aggctttatt aagtaatcgt ctccaccaga atttaaagca tttaaaatgc tttggtcttc
      661 atttatataa gtcatataaa caattggaca agtagttaca tttctaattt ttttacaaag
      721 agtccatcca tcaccatcac ttagaattat ttctaaaaat attaaatcat atttattgct
      781 aaatattttt ccaatagctt cttcacaatt atatgctaaa tctatactta ttcctttttc
      841 ctccagaaat ccttttagct tctggcaatt atccttgtct ccatctatta tcaatatctt
      901 agtttgcatt ataatcactc ccaaatatca ttattctttt atattaaata agctattatt
      961 ataaaaaaca tagacttaat tttattgtaa aaacagttcc ttttcctagt tctgaatcta
     1021 caaatatttc tccaccatgc tctcttataa tggatttgac aatatatagc cctaaccctt
     1081 gcccttttgg ctcattattg tctctttctg taaagaatct gtcaaatacc tttggtatat
     1141 tttctggagg tatacctttt ccattatcag aaattttaat aattgcaaat ccatcttctt
     1201 tatccattga taaaattatc tttccatcaa atggagtaaa ggtcaaagca ttataaatta
     1261 aattctcaaa tgctcttgta agcatctctt tatttatctt tgcatatatt tttttctcca
     1321 atacataagt ttcaaagttt acaccacatg cttgtgtatc tggcaaatta ttttcatata
     1381 tctctttaat aaaattattc agacatattt tttcactaat accaatgctt ttatcttgtg
     1441 atgtaaattc ttgcaaacta cgtactctat tttgtacttc atttgacttt tgatttatag
     1501 cataaagata ggttcttgtc tcttcatcta catgaatatt accaaccttt atcaaatcta
     1561 taaatgcttg tatagccgaa acaggtgcct ttaaatcatg tgctacatct gccaagaatc
     1621 ttttacgttc atttaacaat gttgtaagtt cattagttct cacttcaact tcatgttcaa
     1681 gattccttgt aagtttttca ttctctttta ttattattct attcctgttt atcatcataa
     1741 cagaaaataa aataacaatt acaaacccac aatattctgt ttgccaccct gtaaatatag
     1801 gctcaaatct attactcgta actgcatcaa ataatatgct aattccaaaa acagtgttta
     1861 ctgaaactaa aatataagaa cttatatgct cagagcatgt ctttttatat attgaaacca
     1921 gcgaagacat taatatatat ataaatacaa tatatttata tatgtctatt aacttcccat
     1981 atatgttgac aaagtacata tcatatggaa gaagaataat tggtatcact atagatatga
     2041 tactcattgt aattcctaat ggtaaaatta aaaatctata cactttattt tcttctattt
     2101 tggatatact tccatttata caaataatac ataaaactac cataaaatat gacaaatctt
     2161 caaccgcata tgtaaagctt accaaaggca gtcctaacaa atgtacaaag ggataggata
     2221 catggatggc aaagaaaaca cacatacatc caaaatataa gtaaatttta cttttgtttg
     2281 aaagaaacca tacagacaat gaaaatatag ctattgcaaa agttgtgaag caaattatac
     2341 tataaaaaag taatcgataa aacatcatat tacttatgtc ctttgtttct cctaatgcag
     2401 gcggataata caaaccacta taataatgtg aaaaattcga tacattgatt ataatttctg
     2461 tatttttctt tgcaataaaa cttactactg aatttttaat tttgggtcta tatttttctg
     2521 ttcctacatc tcctaattta gaaaccattt ctccatttat ccatacttca cttgcggaaa
     2581 aaatctcagg aagctctaaa cttagtagct tatccttgcc ttcatttttt attctcatac
     2641 gataagtagc attcccatat ggtgactgag tcccttttct catagaaaaa tctggatatt
     2701 gacctataaa aacatattct ggttttatat cttgctctgt aaaatcattc ggtttaaata
     2761 ttttatgatt ataaaactcc cagtcatcta tcaaaaagac aagtttacct ttagtgaaag
     2821 tatcattatc tatagttaaa attccattac ttgctctaat agacttatat gtatatttat
     2881 tatcatattt atacagaaga tttaatcctg ttattgccag cagagagact atcaatacaa
     2941 tgagagttcc ttttagtata ttatactttt tcattgtatc aaccacatct ctttcatagt
     3001 agtattaaat aaaacaagaa ctatttatta attacagtat ctcacttatg gtacaaactt
     3061 atatcctttt ccccatacgg tttgaatgta ttgatgctcc ctgcttaaag aattaatttt
     3121 ttttctcatc tgtgcaatat gaacctgcac tgtatgttga tctccaatat caggcatttt
     3181 ccaaacttgg ttatatatct ctatctttga aaatacttgt tctttgttct cagctagaaa
     3241 caataatatt tcaaattcca tagtagttaa atctatactt tctccatcaa aagttacttt
     3301 tcttgaattt acatttatta ctaacgctga aaactttaac acattaggaa ctgatgtaat
     3361 atttttgtac tgcctcatac gagcatatat tctaagttca agttctttta aactataagg
     3421 ttttgttata taatcatccc ccccagctaa aaatccccga actttttctt cttcatgctc
     3481 ataattagat aaaaatatta taggtatgtt aaccttttct tttatatcat tgcacatttt
     3541 atatccatct atatctggca atgaaatatc caaaattata cagtctaaaa ctatactctc
     3601 tactatatcc atagcatctt tagcagtctc tgatgtatat acattaaaac ctatattaga
     3661 aaaataaatt tgatttatgt ttaaaatatc aacatcatca tctacaacca gtaaattata
     3721 cattacacca ctccattcaa aggtatttta attataattc caagttttat agttattaca
     3781 aattataatg ttttattttc atatataact ggtattatta ttttataata tctttacaaa
     3841 ctagactata atacttaagt atttttgaat aattcttgaa tattgtatta aacattaaga
     3901 ttcattatta aatactaatt aaagtctccg attgttggag aatggagctt aaagtcattt
     3961 tcacgtataa atttattatt tttctagaaa cttgtctatt ggctagtttg agtattgatt
     4021 tatatgattt atatggttat aatataaaat aaattgtatt attttggtaa atatattgtt
     4081 acaaaaggtt tatattctgc atctcaatgt ttataaaaac tttattttct tattataata
     4141 ctattattct tttaaaaaaa tttccctttt ccaacaaatg gctactttaa aagccaaaat
     4201 tttacctatc aataaaaatc ttaacgacta atactcttat tttcaaattt tgcaacattt
     4261 ttgttaattt taaaacaaaa aaagactatg caatataaaa attatattca tagccggttg
     4321 caccactaac tccatatatc tctagatgcc caataataag atatacttct cagtttctaa
     4381 aatattttta ttaaaaatct tttatatatt ttcataaatt tcttttttat tctaacattg
     4441 taatattatt ttgtcaatat ttccatattc ttagagataa gatactaaaa tacctatttt
     4501 atctaaagat atttatattt ctacattcaa taatagacaa taccctgcaa gttaaagcat
     4561 catttttatg agcttatctc acagggtatt atatgtttac tttatattat aactcttgtt
     4621 attgattaac tttaataaat cctttttctg aaacaagtgc ttgtccttca tcacttaata
     4681 tataatctat taaagcttta cctttttctg acaaaccatc ttctttatta actaatataa
     4741 atggtcttga tattggatat ttattagcta ttacatttgc atcagttgct tcaacatcat
     4801 ttatttttaa actatttact ttctcatcaa catatccaaa tgatatatat cctatagcat
     4861 tttcatttcc ttcaactgta gtttttatat ttcctgaacc atcacttatt tgtgcatctt
     4921 tagttagttc ttctgaatca aaccctacaa tttcttggaa tccatctcta gttcctgaac
     4981 cttcttctct tgatactatt actattggtg catcatttcc tcctacttct ttccagttag
     5041 taatctttcc tgtatatata cctttaactt ggtccattgt cagatttttt actgagtttg
     5101 cagtattagt tatcaatgct ataccatcta ttgctatttt agtttccttt aatccagcac
     5161 ttttttcttc atcttttaaa tctcttgaac tcattcctat ttcagatgtt ccttctatag
     5221 cattttttat acctccagaa gaaccaatct gttgtacttc tacactaaca ccttgatttt
     5281 tttcttgaaa cttttctccg atagtttcca taagaggacc tattgaagtt gaacctgaaa
     5341 cagaaacttt gcttgaatct actgaatcac ctgaacctcc tgagttagaa ctacatccta
     5401 ctgctaaagc tccaactaac attgtactag ctaaaagaac gcctattttc tttttaaaca
     5461 ttttacttac ccccgcttat attttaattt tttgttgtca tttttctata ttttaataat
     5521 acagattctt tgttaaatga atattagagc agggttaagt aaatgtaaat attgttaaat
     5581 acatgtaaaa acttaatttt atatgattta ttcttaatta gattaacttt ttaaagttac
     5641 tttaggcaga tataaacata atacaactca tattatcaga agtcttcatt gaagctatca
     5701 acctatcaca tcctataatt ctttttctag tttattaact aattcagcaa atacatttaa
     5761 ggcttcctct atagattctt ttttcgtcat atctacacca gcagatttta attgttctag
     5821 tgggtattct gaccctccac tttttaagaa gtttatatat ctatctactg cagtacttcc
     5881 ttctgaaagt atctgttgac ttaaagcact tgcagcagaa aaccctgtgg catacttata
     5941 tacatagaag tttgaataaa aatgaggtat tctcgcccat tctattccta tttgtttatc
     6001 tactattgca gattttccgt aatacttttc atttaatttg tagtatatat tagtaaattc
     6061 atcagcagta agtggttctc cactctctac cctttgatgt acaatttttt caaattcagc
     6121 aaacatagtt tgtctatgaa ctgtagttct aaattgttct aaataataat ttaataagta
     6181 tatagtttca tccttagact tagaattttc taataaataa tttataagaa gtaattcatt
     6241 taatgtagat gcaacttctg caacgaatat cttatagtcc gaatataaaa atggttgact
     6301 tgttcttgaa taatgactgt gaacagagtg ccctaattca tgaactaaag tgaatagaga
     6361 attaaggtca tttttatagc tcattaatat ataaggatgt gaatcataac ttccccaaga
     6421 ataagctcct ccttttttac cttcattatc atatacatca atccaccctt catcaaaagc
     6481 actttttatg accttcaagt attcttctcc taaaggttta agtgcctcta atatgatatc
     6541 ctgtgcttta tcatatggta tatccatgtc aaatttatct gtcaaaggaa catacaaatc
     6601 atacatgtgt atttcattca atcctaaaaa tttcttttta agctcaacat atttatttag
     6661 attaggaata cttttatcta tagctgatat aagattattg taaacatcta aacttacatc
     6721 atctgaaaat aaagacgctt ctattgctga attatacttt ctagtttttg cgtaaaatat
     6781 ctctgatttt attccaccat aaagcgttga ggcaaatgta tttttatatt tctcatatgt
     6841 tgaaaattca ccttcaaaag cgtcttttct aactctacta ttcttacttt ttataaataa
     6901 agagtagttt gaatgagaaa gcttaacctt attcccctcc tcatcttcta tctcaggaaa
     6961 ttccatatct gcatatgcta acatatcgta tacattttct ggaattgtcg ataattctga
     7021 tgtagctgca agtatttctt cctctcgttc actcaaagta tgtggtttat ctctcaatat
     7081 atcctttata tattttgtat aaaaggatag cttctcatct tttaagtatt cacttaactt
     7141 attctcttct atcgccagta tttctggaac tatataagaa gtagccacac ctaaatcagt
     7201 agataacatt tctgtcttag tggctcttcc ttgattatca ttaattctag tatcttcatg
     7261 ttgtttcata tgagtatata catataaatt ttgaacaatc ctagatgcat tttcagagat
     7321 atttaatact ttatataagt tctccttact ctcagaaagc ttccctttat attcttttac
     7381 ttcttggata agatttttta ctttacttat gtctttttca atttcctctt tactggagta
     7441 catcttatct atcttccatt tgtacttttc ttctattgta cttctatcag ttcccatatc
     7501 atacacctct attttaattt gttttatatt ttattcactt tacaaaatat attgtatata
     7561 ttttagtcca aataatctac atttattact ccttctatca tatttatttc tttcataaca
     7621 tcgtgtaagt gtattctctt agcataagat acaaaaaaat taaccttcca gtattcttca
     7681 ttacgacatt ctatttccat atttcttacg ataagttgat ttttttcaaa tttatcatat
     7741 aaaaaaggta tagagtccaa attgcttaat tttaatatca atacattctt ttcttggcta
     7801 aataaaaacg tattgattat cctaagcaca aataatgcta tcattacaaa taatccacct
     7861 agaattgcca attcataata accatatcca actgctatac caatacatgc agttgcccac
     7921 atcccagcag ctgtagtaag cccttttacc ccacctttat tttttaaaat tgctcctgca
     7981 cctaaaaaac caattccaga taatacttga gcaggcattc ttgctgggtc agcatttgta
     8041 tacaaattat aatcaaagaa taattgaatc gaagttattg aaacaataca agaacctatt
     8101 gcaattagta tatgtgtcct aaatcctgca aactgtctta ttttttctct ctctaagcct
     8161 attattcctc ctattattag tgctaatagt aaccttattg ctatctcctt acttcctata
     8221 tcaatcataa tataaaaccc tcctgactta attacaactt atgtcctaca gataatttac
     8281 acatcagaaa tactatttta tatcttaata ttattattaa tatatgtaaa atatagccat
     8341 ctgttaattt ttgatacaaa aaaacccaca actgtgggct atttgcttag gtacatgtat
     8401 ttatttatta atatatctat tttcttactt gattctacta actcttcttg aaatatttct
     8461 ggatattcta tgtatgtatt tatctcttcc ctaactgctt caatctcatt ttttaacgct
     8521 tctaattcat tgttcgccat aataatcacc actattacaa atttgtatat ttttatatac
     8581 attttatcaa acaatattat attttgtcaa tattatgtca ataaaactta ctataaaaat
     8641 gctttaattt aggtattgta tacctggata ctaagcctac aaacatacca gttactaatg
     8701 atgccattaa cataaatggt aagtacccta tagcaagtat attttctatg attaaacttg
     8761 caactattaa ttgacctata ttatgcccta ttgcacctat aatgcttatc cctattagtg
     8821 ataaatcttt tattttactc gctaaataca taaataaaag actcagataa gctcctccta
     8881 tactaaatag tagatatgat aaagggcctg caaatattga tgacaaaatt accctgatag
     8941 taacaattat aacagtatct ttgacaccta acaacattaa tgaaattaaa gttataatat
     9001 tgctcaaacc caattttgcc cctggaaata taactataag aggatttggt atatatgtct
     9061 ctaatacata taaagctaaa ctatacccta tcataagacc taaaaatgtc atttttctag
     9121 ttttcattaa gttaactcct ccaaatttta tctatcatct tacattataa tacaaaatat
     9181 ataaaaacac ctaatttaga ctaaaatatt tttaatctat ttagaataaa tattagttat
     9241 ttcaatgtgc aattacctct ataaatcttg actttttgta tcatacttgt tacaatttta
     9301 atgataatga atttcattag gaggtgttaa taatgactgt ttacgattta aaaataggag
     9361 aaaaaggatt tattaatagt atagatggag atattaaatt atctaaaaga ttgcttgcac
     9421 ttggatgtat aaatagtact gaaatagaag ttaaaaaggt tgccccactt ggagacccaa
     9481 ttataattag atttagaggc tttgaccttg cgattagaaa atctgatgct aaaaatattt
     9541 ctttacaata atagaggagg agctacccgt gattaatgta gcattattag gaaacccaaa
     9601 tgtcggtaaa actactgttt ttaatctact tacaggttca aatcaatatg taggtaactg
     9661 gcctggtgtt actatagaaa aaaaagaagg atttcttgga aaagagataa aggttgtcga
     9721 tttacctggt atatatgcaa tggatacttt ctcaaatgaa gaaaaggttt ctaaatcata
     9781 tttagaaaat gaagatgttg atgtaatagt aaatgtagtt gatgcttcta atttatcaag
     9841 aaacctttac ttaacaactc aacttatgca atttaacaag cctatagtta tattgcttaa
     9901 tatgttagac attgcagaat ctaaaggagt taatatagat gccaaaaaat taagtgaaga
     9961 acttggagta attgttgttc caattatagc aaaaaagaaa aatggtgttg ataggataga
    10021 agaagagatt aagggagcta cgcaaaaagc ttttatatat cagaaaaact ttggaagtga
    10081 aacagaaaca tataaaaaat tagaatccat tttatcgaga tgtacaaaaa ctacatatac
    10141 acaaaagaaa tctatcagtg ataagataga caatatagtc ttgaatccta tattagcata
    10201 tccgatattt attggtgtat tgttcctctt atttaaattt acctttgact gggtcggagg
    10261 accactacaa gagggatttg ctagtcttat agaggcttat atttctgcac ctgttagtga
    10321 tatgttatct aactctagcc catggtttaa atcacttata gttgatggaa taattggagg
    10381 tgttggtggt actttaccat tcttcccttt aatatttaca ttattctttg gtatatcgtt
    10441 gtttgaagat agtggataca tgtcaagaac agcattttta atggataaag ttatgagaaa
    10501 ggttggtcta tctggtaaag cttttatacc tatagttatg ggtatgggat gttcttctcc
    10561 agctataatg gcaactagaa cattggaaag cgaaaaagat agaagagtta cagctcttat
    10621 tgcaccactt atgacttgtg gagcaaagct acctatatat gcactatttg tagctatatt
    10681 ctttccacat aatgctgctt tagtaactac atcactgtat ttggtgggta tagttatggc
    10741 aattgtagtt gcattattct taaataaaac agcctttaaa acagaggctg aaccatttat
    10801 attagaatta ccagaatata gaatccctac tatagatgca cttattaaaa atacttggaa
    10861 taaatcaaaa ggtttcttga taagagttgt tactgtcatg tttgcaatgt cagtcgtaat
    10921 ttggggactt tcttcattca atgtttttgg atatacagaa gatataaatg taagcttctt
    10981 ggctactcta ggtcatatta tatctcctat attcaaacct cttggttttg atgattggag
    11041 aacttcagtt gctattttaa gtggtcttgg agcaaaagaa atcgtagtaa actctttaaa
    11101 tatactatat ggtaatctaa ctgttgtctt accaacaata tttaatggag ttactgcata
    11161 tacattcttg atatttactg ctttatatac tccatgtata gctgcacttg caactatgaa
    11221 aaaagaatat ggcaataaaa tgatgtttac atcatttgca tatcaattta tactagcatg
    11281 gataatggct tttatagtta aaagtatagg tggagtacta tttatgggta attcaataat
    11341 ggaagctttg ataggtggaa taattatagt ccttgcactc tttattctgt ttaataaatt
    11401 caagtctaca aaaaacggaa atgtctgtag tggatgttct atttgttctg gatgtccaag
    11461 tgctaattct tgctcaagta aggaagacag caataaaaaa aataatgcac actaatatat
    11521 aaatatttta gtttaaaata agataaaagt gggtgtctca aaattaactt tttagttcat
    11581 gaggcactct tttttatata aaatcaaata tattttcatc atttcaacag taaaaaagag
    11641 cttatctagt aaaaaagagg cttatctcta ttttgagaca acctcttttt ttgttactta
    11701 ttcagtttct tgctacctaa cttcatgtgg aacctagtat cttttggaac acttgggtct
    11761 acacctctta atgtaggtat ttccccacat attagttgta taggtactat aaaattaaat
    11821 gtttgatagt aaggattatc tgtgatatca atctttaaat ttttatcatt ttctgtaaca
    11881 tctctaccta tagcaaatac attttccgtc caaccagaaa gtacatcaat catcttagtt
    11941 actctaggtt cttttcctgt atctagtata aatatagtag aatcactatt tatagcatta
    12001 tatataccat gtataaactc ttcaaactca tacccagtta caggtattct cattgtttcc
    12061 aataatttta atgctgcttc taatgtatct ccatatatat cagagtgacc tattattctt
    12121 atttctttag aattaactaa cttttctttg tttctttcaa tccattgttt agagagctta
    12181 tacactgctt caaatctatt aattgcgtct aatatcttat ttatttcctc attgtacttt
    12241 tcactagata ttataccttt ttctctagct atttgaagac ctaataacat taagtttaac
    12301 tttgtacaat aatatccttt tgttttagct ccagattttt cttcaccaca atttactgtt
    12361 aatatataat cagaaatctc atctattaaa gcattcttac aacctgccat agatgctatt
    12421 ttacagccct tatcttcagc taacttcata gcgttgtatg tagaataact actcccacct
    12481 tgagatacac ctactactag ggtattttca ttatcaaact tgaatgtatc ttcagttatc
    12541 ataaatggat acattttcac aacatctata tctaataaat tttgtaaata tggttgcacc
    12601 tgcactcctg agtgatagct agtacctgaa cctgttatta taatcttttt tagatttgtt
    12661 ctcttaactt cattaaataa gctatctgca ttagatataa tttctctcat tctaactggt
    12721 gtttctaaca tataatcttg tattgtcatt ttatacacct tccttatcta tatattcaat
    12781 aaattataat taatttctat aacaaaccta tagcatgtaa agcgattcct gttactatag
    12841 tgcctatagt taattttgtt acacttacct ttctctttaa catgtagtat attacaaatg
    12901 caaaggttaa aggtaacata ttaggtatta ttttatctag tatatcttgt aaaattactt
    12961 ctgcaccatt taagttgaat ttcagaggag tagttatact caacatagtt gcaaccattc
    13021 ctcctacaac gaataaacca acagttgtag ccatagacat tattttctcc ataagtccct
    13081 ctctttgtat tctttcaaga gaatttaccc ctacttgata accatactta agtccaaata
    13141 ttctaagggc aaatgctggt atattgtata atagtaagaa taatagtggt ccaaagatat
    13201 taccttcttt tgcaagtgat actcctactc ctgctgctat aatcctaaat gttccccaga
    13261 ataatgaatc accaattcct gcaagaggtc ccataagagc tgttttaaca gcactaattg
    13321 aactttcatc aaattctgga ttattagcat tctcttcttc catagcggct gttataccta
    13381 atatagcagg aactaccact gtcgttgtat taaaaatctc caaatgcctt tctattgctt
    13441 ttgcttgatt ttctttctta ttgtatagct tctttattgc aggtaacatt gaataacagt
    13501 aacctaagtt ttgcattctt tcgtaattaa atgcaccttg aagagcaaat gaacgccaaa
    13561 acatacttct taattctttt tttgttaatt tattcaaagt caatctctcc ttccgttaaa
    13621 gtattgattg ctaattgagg attttgatta ttttctttgt tattgatata aaagtttagt
    13681 acaaatgcta cacatactcc aaataaagct atagcagtta tatctaatcc accataagat
    13741 gctaacaaaa accctataaa gaaaaataca gacatctttt ttgaaagtaa catatccata
    13801 agcaatgcaa aacctactgc tggcaacaat gttcctgcaa cacccaatcc gtctaatata
    13861 actttaggta ttgcatttat taatgctgta actttatcag caccaatcat aattgctaaa
    13921 aaggttggaa ctgatgtact taaaaagaat aatattactg gcaaccacat cattatggta
    13981 actttcttaa aatcagcctg tttagcatag aaaactgcct tttgagaaaa gtacgaattt
    14041 attattctaa ctaatactcc aagagattgt gctaagactg ctactggtac tgctactgct
    14101 aaagcaactt ctgcaccatt acctgataat atagcaaaag ctgtacctaa tacacctcct
    14161 gttactacat ctggaggagt agctgcacct attcctgcaa ttcccatcca aattaattca
    14221 agctgtgcac caatcataat tcctgatttt atgtctccta aaactaatcc tactaacata
    14281 cctgtaacta ttggcctatc aagcatagat tgaccaaata tacgcccatc tagtatacca
    14341 actcctgcta taatagctaa catagtagct tgtactaaca tattgaacct ccattttaac
    14401 ttagtttaat ttacattaaa ttacattaca tttttgattt tgttttgatt ttctaataac
    14461 tcatttacat aaacaattcc attttttgct tcctctaatc ctgatttaac tattagtttt
    14521 gtatcctcct caactaataa aagtatagat aaaagcattg gtaaatttac tcctgtcaat
    14581 aattcaaatt tgaacccttg taaaagtaag tttgtacata tgtttgccac acttccacca
    14641 aacacatctg ttaagacaac aaattcttca ctatctttat tatttgataa tatacttcta
    14701 atttctttct cagcatcatc tgctgattta tccgttgtca tacaaaaata ctcaatatta
    14761 gttaatgaac caattattaa tttacttgtg tcaacaagct ctctggacaa ttctccatgt
    14821 gttgcaatta gaaatttttt cattctcttt ctcctaaatt attttattaa atctgatagt
    14881 tgtttctttt tgtctctagg taacattcta aattctatat caatccctaa atcacctatt
    14941 tgtttaaagt tatttatatc atcatcatca acagcaacta aatctgtaag tttcttcttc
    15001 ccttcctcat atcttaatcc tcctacattt atttctttta atactcctcc tgaattttta
    15061 gctacaacta aagcatcttt ggtatttttt actaacacta atacattgct tttctttgaa
    15121 gaagcaaatt tttctactat ttctccagat tcctctaccc cccttatata taaagttaca
    15181 ttgctaggtt tcgctaagtt cagtgacata gctttcattt ggtcattttt tgcttcatca
    15241 tttgcaacta gtattacatt tatacctagt gatgttgccc atccatatgc aacctgacca
    15301 tgtatcaatc tgtcatcaat tctcatcaaa gataccattt tccccaaatc ctccatcata
    15361 cattaaaatt tttattgttt atactctcat ttatctataa aagcaaaaag tatgccaaat
    15421 actggcatac ttttttaaat ttcattcact tattttgtaa tcttgtattt tactgtggta
    15481 gaccatagat aatattataa atatatccta tctctgaaat tggtatttgc acactataac
    15541 tcttttctag cacactaaat gcatttttta ttagttttac ttcactatga tggtttttga
    15601 ttaacaaatc catatcagta tactcttcaa tttcagtttg tctaactaat ctttcaatca
    15661 tacaacttaa gtgaacatat aaagaaatct ttttatcatt tgaaagtttt ttcttttgaa
    15721 tattttctat agcattaatt ccttcttcta ttctctctat tatctttttt gaatctaaaa
    15781 tagttagact atctattact ctaattaatg aaaaattctt tacaatagaa tcatttatgt
    15841 tctttatttt ttcattttct actactctgc taaatattct aaaaacatca tcttctcctt
    15901 ttcctgatat tatatcctct aataatataa aatctacccc attcacattt ggattggcag
    15961 ttcctactat agccatcaca tcatacattt caaaaactgg ttctttatct cccatagaaa
    16021 caagactatc ataatcataa ggtattattt tgacatcaac tattccaatt aaactttcgg
    16081 aaagtagatg ttggattttt atagcaatac ctatccctgt aaaacaagat gatattatag
    16141 catattcttt atttttttga ggataataca attgtatatt aggtttagtt tcttcatcca
    16201 taatttctgc aatctcttca atgtttttac cttgcattaa taaatttcct acttctagtg
    16261 cagaaatagt ggatatttgg tctataaata acattgggct atttatctta ttctttaaat
    16321 cttcatatat aagattaaga gagcccatat caaataatac aattaaacca ttctttatcc
    16381 tatctccctc tagatatcta gacatttgtt ttgatacatc agcaatagta gcatctatag
    16441 gcatatcaat agctgtaaat atatttttac ccaacatccg attacataca ttcgccatac
    16501 tactggcagt agcatatcca tgtgctataa tagtcgcatt aacttgatta aatttcattc
    16561 ttataattgt tgatttaata aatataatca ttatgattgt atctacatta ttaagataca
    16621 tatccaatct attcgttatt aatgttgata ttttacttat tatagagtat tctttgtaac
    16681 tctctttttt taaatatatt agtaatttat tttctaaagc tttatactca tcagacaatt
    16741 gataagattc gtccttcaaa taaagataag acgaaaatgc caatacatta ttcccatcat
    16801 attttatatt gttattaact tctaaatttc taaatatatc ctgcattatc gatattataa
    16861 gctccataag tacgttatta ttttcatcat tatttgaaaa gactaaattg tccattagtc
    16921 taatcattag attagaactc tttttactaa atttttcctt tgttatttca tttttttcta
    16981 gtctatcaaa gtaatctaaa cattttatga aactttgata gattatattt cttttatttc
    17041 ttccatttga catttcaata ctaaattgtt taggagaaaa gataatgctt ttgcttttac
    17101 cactttggaa ttgaaatata tctacatttt ctatataaag atttctagga aggtcttgaa
    17161 gagtaacaaa aatatttcca tttcttcttt gattttttgc ataagctcgt ccacatgcat
    17221 attttattac atttttacat tcacctatat tccccttgaa ttttatcttt aaaatcgcat
    17281 ttaatgtaat ttgagttatc tctataggca aaccaagtac cttactttct tcataaataa
    17341 aatgttctac aatctttctt ttttcaatag tccctctttc ttctatattg ggaacataaa
    17401 tatttatggg tattcttctt aaaaaggttt gtaaaaattc agatagactt tctgttgtag
    17461 caaatattaa tcttacccta gcttttctaa ttactccatt ctctccaatc ctagaaaacg
    17521 tacctgtatc cataaatgta aataactttt cctgaccttc actatttaat ctatggactt
    17581 catctaaaaa taaaacacca ccatctgatt gttctattag acctgctcta gattcatttg
    17641 ctccagtata tgctcctttt acatgcccaa ataatagact agacaataac tccaaattat
    17701 tataatactg cgcacagttt aacacattgt atggagaatt tttttctaat acattactgc
    17761 ttacactata tttgtatatt atcttggcta aaaaagtttt tcctactcca ctttcacctg
    17821 tcagcattat tgtaagtccg ccatttggat aaagtacagc ggtttttatt tgttcgatta
    17881 cttcttttaa gctattatca tatcctatga tactattaaa cacagtctct tgctttaata
    17941 atttacactc atttttaagt tcatctaaat tttcataaat actttttttt gtaggtccta
    18001 ctacatcatt tattttattt ttaggtaaaa aaataacagg tctactgttt atctttataa
    18061 gattatcaga ttttactaaa tcatttaata atctacttga ttgagttcta cttaaattaa
    18121 acttttttac tatatcttct gttattatgc cactttcttt tttaagttca tcaaagctgt
    18181 taaataatac acattcattt acaaaactta aaatttcatc cctcatgttc aacctccaat
    18241 acaaatatca ataatactat aatattagta tatcatttat atatttatga aaaagataat
    18301 tatattccta tcttgattct cttttgtaag gttgtaaaat caaacttttt ataagtataa
    18361 aatctagatt ttttaacata atctcaaggt tcttatcttt ccatatatct gacatagaa
//
LOCUS       DAMJWS010000085        18397 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMJWS010000085 DAMJWS010000000
VERSION     DAMJWS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.086"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(477..1349)
                     /locus_tag="PC399_002990"
     CDS             complement(477..1349)
                     /locus_tag="PC399_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2593836.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1743..2321)
                     /locus_tag="PC399_002991"
     CDS             complement(1743..2321)
                     /locus_tag="PC399_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2593837.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2379..3095)
                     /locus_tag="PC399_002992"
     CDS             complement(2379..3095)
                     /locus_tag="PC399_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2593838.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3286..4371
                     /locus_tag="PC399_002993"
     CDS             3286..4371
                     /locus_tag="PC399_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2593839.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(4526..5185)
                     /locus_tag="PC399_002994"
     CDS             complement(4526..5185)
                     /locus_tag="PC399_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593840.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNSNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(5402..6694)
                     /gene="lysA"
                     /locus_tag="PC399_002995"
     CDS             complement(5402..6694)
                     /gene="lysA"
                     /locus_tag="PC399_002995"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF2593841.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(6712..7905)
                     /locus_tag="PC399_002996"
     CDS             complement(6712..7905)
                     /locus_tag="PC399_002996"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2593842.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(8266..8883)
                     /locus_tag="PC399_002997"
     CDS             complement(8266..8883)
                     /locus_tag="PC399_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593843.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(9053..10753)
                     /locus_tag="PC399_002998"
     CDS             complement(9053..10753)
                     /locus_tag="PC399_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593844.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(11150..11713)
                     /locus_tag="PC399_002999"
     CDS             complement(11150..11713)
                     /locus_tag="PC399_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593845.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(11872..12585)
                     /locus_tag="PC399_003000"
     CDS             complement(11872..12585)
                     /locus_tag="PC399_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF2593846.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            12877..14541
                     /locus_tag="PC399_003001"
     CDS             12877..14541
                     /locus_tag="PC399_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF2593847.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(14648..15547)
                     /locus_tag="PC399_003002"
     CDS             complement(14648..15547)
                     /locus_tag="PC399_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF2593848.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(15574..16212)
                     /locus_tag="PC399_003003"
     CDS             complement(15574..16212)
                     /locus_tag="PC399_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2593849.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(16549..17547)
                     /locus_tag="PC399_003004"
     CDS             complement(16549..17547)
                     /locus_tag="PC399_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF2593850.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KKISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(17645..18220)
                     /locus_tag="PC399_003005"
     CDS             complement(17645..18220)
                     /locus_tag="PC399_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593851.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         5960 a         3057 c         1943 g         7437 t
ORIGIN      
        1 agtattatat ttctaaaaaa gtttcccttt ccccaaaaat ggctacttaa aaaatcaaga
       61 tttcatacct aactactaat tctatgattt ttaaattttt caacttcttt gtcaaagtta
      121 aaataaaata ctaaaagaca catattatag tgaacaaaaa agatagaaat acatatttta
      181 atagaatttt aataagatat tatatactta aatacaaata aaattaattt tataattgtt
      241 aattaaggaa atgatattgt ttagagaaat aaaaaaagca ctctccatga agggaaatgc
      301 tttctttaat aaattagttg atgtagtaaa gatatattca gaatacatct ttatgttttg
      361 agtctcgaaa tatttatttg tattatttaa acgaaaattt attttttaag tgataatcta
      421 gtatttatta ataaggctat gattctcaat ttcatagcct tattaataca caaactttac
      481 tcatacacag aatatctttt ccaaatttgc tctaaatcat caaaatgctg tttaatatct
      541 tctttttcct tcatcccaac agatatagca agagcatttg aaatactaag agctggaact
      601 agagaatcta caaatgaagc catattactc ttaactagaa gagtgttatc tgcaagtgat
      661 gctactggcg caaataagct atctgttaat gatattacat gggctccttt ttctttagca
      721 taattgacaa tttgataaga tttcttagaa taacgaggga aactaatagc aactattaca
      781 tcttcttcat ttattcttac aatctgctca aaagcatctc ccatatccat tctaattata
      841 tgaacattgt caagtattat atctaagtaa aaacctaaat attgagctac tacaaaagaa
      901 ctcctcattc caagtatata tatttttctc gctcttaaaa gtttgttaga agcttcttta
      961 aacgctctct cgtcaatttc ttctagtgta gaccttatat tatctatatc actttttaaa
     1021 actttgttta gtattgcaaa atcatcggaa taatcatgtg ccatttcaac tctttgtact
     1081 gtagttagtt tattttttat gagttcttga agtgcagctt gtaatttagg atatccacta
     1141 taacctaatg catttgcaaa tcttacaact gtagactcac ttactcctac agtttcaccc
     1201 aatttacatg cagtcataaa tgcaacttta tcataattat ttaaaatgta ttgtgctata
     1261 agtttttgac ccttactcaa tctcgtatat tgagattgta tatttgatat caaatgttta
     1321 ctgtccttca tttcatttgt ttcttccatt tttttctcct tttagttata taaactattt
     1381 tgttttatta acactgtctt tttctctaaa aattttattt tttatctcaa tttcatctta
     1441 gtttttatca tagcttctta aactcataaa attctaactt ttctgtacct tatacttcac
     1501 atagtatttt cgattaaatt tattatacca tctttccttg cattttttaa tatttatcca
     1561 atatttcttt taaatttttc aatttttact catttaaaag ttatctatct tattttgcat
     1621 atttagatgt atattttgtt ttgagaataa actgttgtat acttattatt taaagtattt
     1681 ttaataggtc acactatata tattaactct taatacaatc ttgtcaagtt ttttagtatt
     1741 tattatgctt ttttcttttc aattaaaatc aattgtggtg ggttattgac ttgatttaaa
     1801 aacttagatt ctaaaacatt aaattcatct tgatttaaat tattgacaag gttatatatg
     1861 caatcttttt cttcttgacc tccatcatgc cctgtatata tggcaatgct tattactcca
     1921 ttttctttta acatttctaa acttaacttt atagccttta aagttgtatc tggtttggtt
     1981 ataattgaat gtttcgctct tggaagataa cccaaattaa ataaaacaca tgaaacttct
     2041 tcttttatat attgattcat tttttcatgc ccatcaaata tcaattttac tctatctgta
     2101 tatccttctt tctctagttt ctttcttgta gactttattg cttcttcttg tatatcaaaa
     2161 gcatatacaa agcccttttc tccaaccttc tcagctagat accttgtatc atacccattt
     2221 cccatagtag catcaattac tatgtcacct tcatttataa tattttctaa ataaaattta
     2281 tttatatctg taattttagt taaatacttt gcatatgcca taatactatt tctccctact
     2341 attaacttta tttcattttt tctcaattta tttttatctt attttattga ttttaaatat
     2401 tttatttcat cttctgttag gtatctatat tctccaactt tggtgtcttt taatactatt
     2461 ttccccacag aaatccttct taaatttaaa actggatgat ttatagcttt gcacattttt
     2521 cttacttgtc tatttctacc ctcatgtata gttatttcac acactgaata atttttttct
     2581 ttattttctt tagttaccct tattttggca ggtgccgttg tgtaatcttc tatttttagt
     2641 cctgtttcga attgtcttat ctcatcatca cttataattc ccttcacact tgccacatac
     2701 attttatcaa tttcatgctt tggatgggta agcttatatg ttaaatcgcc atcattagta
     2761 agtattaata aaccactagt ttcataatct aatctcccta ttggatatac tctttccctt
     2821 acatctttaa gtatgtctat tacactgggt ctattgaatt ggtcttttac tgtagttata
     2881 tatccctctg gtttatttaa tactatatat atataatttt cacttggttt tactttttta
     2941 ttatcaaatt ctactatgtc tttttcttca tcaatattaa aagataactc ttttactaca
     3001 cttccattta cctttattct tcctccaaga attatttctt ctgcttttct tcttgatgct
     3061 attccacaag aagctatata cttattaatt ctcatactta atccttcctt atcttaaatt
     3121 atgtgtgttt aatattttta tcaatgttaa ttttgctaca ttattatact atactagacc
     3181 ttaattaaaa acaagcaaaa aaattattta attttcctaa ataattgtaa tttcttttca
     3241 catactatta atatgtaata gctgactaaa cactatttta aggaagtgat tattatttca
     3301 aaggaaaata taaaatatta tttaatagtc gtgtttattg gaattttatt ttttaaattt
     3361 attaatactc catcagactt tatatctagt attgaaggat tcttaaagtt ttttagccca
     3421 tttttattag ctattttact tgctttactt ttaaatccac ttgtgatgct ttttgagata
     3481 aaattcaaag cacatagact tttagccatt tttttatctt atatatttat aggttttatt
     3541 ttagcctttg ccattaggtt attaattcca tcaatagcta atacattaaa tagattgata
     3601 aatgaaatgc ctatgtatac tgattatata gatagtttta tagaaaaaaa tatgtcaaac
     3661 atatattttc taaaaacttt aattcctcat atacaacata gtttagataa tttattaaaa
     3721 gaagcaagta attttgttgg taaaattcct aaaaacttct taatctatac acttagtata
     3781 tcttctatgc tatttaatat gacaatggga tttatactat ctatatacat tatatatgac
     3841 aaagaaaaaa ttgcattggg atttaagaga tttctatatt cttctactgc tagaaacaag
     3901 gcagataaca ttatagaatt ttttaggact actcatgata ttttctatga ttatttactt
     3961 ggtagaatat tagactctct tattattggt ataattgcat ttttaggatt tcaatttgtt
     4021 atcagaatag aaaatgcttt atttttagct tcaataatat tcttaggtaa tataattccc
     4081 tattttggtc cttttattgg tgcaatccct ccaatagcca tgactatatt atatagtcca
     4141 caaaaaacta tatgggtcat tgcatttcta tttatattgc aacaattaga tggaaatttt
     4201 atagaaccaa aagttatggg taatcaagtt ggtataggag ctatatgggt aatatctgct
     4261 attttggtgg gacaatctct atttggtttc ataggagttt ttctttcagt accaatagct
     4321 gctgttgtaa aaacttatgt tgataaatat atagataatc gtttacaata gcttatctct
     4381 actgtgactt taatttttag tttaaaattt aactgttatt tattaaatta tttctcaaaa
     4441 aatatctctt agtaagaaaa aattgctaag agatattttt tataatatat aatttgttca
     4501 ttaaatttat gcatatattt tatacttatt gattatttga gttttgagaa tttccaccac
     4561 tatctgaacc accagtagta tcttgtggag ttggtgctgg tgttggatta ctattatttg
     4621 gcttttcttg attttgagaa gaactgttac tattgtcaga cttattacta tctgattttt
     4681 tatcattatt tttagaacta gatttattat ttttactact tccactataa gaagatttcc
     4741 ttgcatctga ttcaactcta tacctatctt ctccagccgt atcttttgcc tttttctttc
     4801 tttgttcctc tgcttcgttt tgttcttttt ctaatttagt taactcttct ttctttttag
     4861 ttgcctcttc tttaataaca gaggaaccat ttttgatttt ctctataagt ttaagttcct
     4921 tgattgcacc ctcataattt tttaattctt caaactccgt catatttgac attctcatag
     4981 cttgcatata catagctctt gcattctcat tagtactatc ttcttccagt gcagaggata
     5041 atgattccat agctttttta tagttatgtt tttctaaata tgtttttcct tgctctacta
     5101 aatttatttc atctttttta ttaaatgaac atcctacaag aaatatagat aatatcgata
     5161 ttaataaaat tactcttcct ctcattaaat ccctccttgt catatacttt aaattttatc
     5221 ataattataa agtttttaaa atattttata tttattacaa gaaatttgtt agtaaaacaa
     5281 aaaataagca gatatgctac gaacgataca ataaaccaaa aattaattta gagatacata
     5341 tcctagcaaa tctgctcata ataaacttta atatttaatt tttattatat acttctccat
     5401 tttatatctc taaagctaat aagtcctcat agctttgtct cttacaaatt atcttatcat
     5461 ttccatcaaa tactgatact actgctgctc ttggtatttt attataattt gaagacattg
     5521 aatatccata tgcaccagtt gatgttgtta taagtatatc tccactttta atatccatta
     5581 tgtttatatc tccaattaat atatctccac tctcacaaca ttttccagct attgttacat
     5641 gattcatagc ctcatgatta attttattca caatactaca ctcataacta gcttggtaca
     5701 acgaaggtct tacattatct gtcatacctc catctacact tacataaacc ctaacatctt
     5761 ttatatcttt tatagaacct actgtataca atgtacttcc tgcatttgca actattgacc
     5821 ttcctggttc tattaccaat gtaggtactt ctattccaag ctctttacat gaagtttctg
     5881 ctttagtgat tatggtttca caaaactctt ttatagtttt aggtttatct ccttcgctat
     5941 aataaacgcc aactcctcca cctaggtcaa tttctgttag ttgtatgttg aatttttctt
     6001 ttatttcttt taccaagttc atcattatat ccactgtatc tatgtatggt tctacatcaa
     6061 atatttgtga acctatatga gcgtgaagac ccacaagttt aatattttta tattcactta
     6121 atttttctat tgctctaaat aaatctccat ttactaaagc aaatccaaat ttagaatcta
     6181 tctgtccagt ttttatataa tcatgtgtat gagcttctat accaggagtt attctaaagt
     6241 atatctcttg agttttacct ttatcttcac ataatgcttc aagcaaatct aattcataaa
     6301 aattatccac cacaaattta cctacaccta aatctaatcc catgtttatt tcttctttag
     6361 tcttattgtt tccatgaaag aaaacttttt ccataggaaa atttgattta taagctgtat
     6421 ataactcgcc tcctgaaact acatctaaac acatttgttc ttcatttata agattacaca
     6481 tgtaaagagg taaaaaagct ttccctgcat aagcaacctt attttttccc tctctagcct
     6541 taaaatattc tacatattct ctacaatttt gtctaactaa agcttcatca aatacataaa
     6601 gtggagtttg atatttttta ctaagttcta aacaactcac tcctccaata tataattcat
     6661 cattatttac agtcatagtt ccatgaagtt tcataatagc ctccccaaat tttatatatt
     6721 aaatacttct gctatttttt ctatagctac ttgcttatca tcagaattta tagcacaagt
     6781 tatacgaatc tctgatgtag ttataagttt tatcattata ccattttcat tgaatatctt
     6841 aaatacttta gcagcaactc cagaagtatt tttcatacca agacctacta aagaaaattt
     6901 agtgatatta ttatcaatca ctacatgttc ttcatctgta tatttagata ctatcttttt
     6961 acattctccc aactcttctt ttggaactgt aaatgataca cttatcttat ctaaaatagg
     7021 tgcagtttgg cttatcatat ctacacttat acctatagtt gctatatctt caaataagct
     7081 agatatattt tcagctttaa aatcttttat tgttatagaa ctgtcatcat cactagtagc
     7141 taatcctgtt attactttat cctctaattt catgtctttt ccccctctta tatatgttcc
     7201 tttttctgta ccacatgtac gtccgacata gatttcaaca ccatattttt gtgcaagctc
     7261 tatacttctt gaatgcatta cttgtgctcc taaactcgct aactcaagca tttcttcata
     7321 ttcaatctca tcaagtttac tagcctttga atattttctt gggtctgtaa aataaattcc
     7381 atcaacatct gtatatatct cacattttcc atttaattta accgcaagag ctacagcaga
     7441 tgtatctgaa ccacctcttc caagagtggt tacatcacca tcttcgttga ttccctgaaa
     7501 tccagttact atcacgacat ttccttcatc taaactattt ttaattctgt ttacgtttat
     7561 atcatcaatt tgagactttc catgaagacc actcgttttt atatttaact ggtaagcatt
     7621 ataacttata gctttacatc caagtgcatt taatgctatt gataataaag atgctgaaat
     7681 catttcacca gtagacatta aagcatctaa ttccctctta ctaggttcat tactcaattc
     7741 ctttgctaaa gttatatatt catcagtgga ttttcccatg gcagacacaa ctattaccat
     7801 ttgtggattt tcttttcttc gttctattat attttctgct attgatttta ttttttcagt
     7861 atctgctact gaacttccac catatttttg aacaattata ctcatgcttt ctccccctca
     7921 agctaaattt tgataaataa aaacgcccaa aggtatactt tggacgtgta aatttcatag
     7981 accaagtaac cttgtagctc accactttaa aagtgacagc cttatgcata ttctgcataa
     8041 gtccagaaaa tttgtccgtc aacaaaattt cttcggctat ataccctttc atcaattatc
     8101 aacgcttacc agctcctatt gaatactatt gaatacagct acctctaccc taggctcatt
     8161 atttttaatt aatttattaa tatagtttta tcatattcta tataatattg caacaattat
     8221 tttacaaaat gtggcaaatc gatagttttc ttttctaaag ttagcttata tacaattaaa
     8281 tagtttattt aatcctaaat ctataaaatt tatgctatac tcattatcat atctctctaa
     8341 atcatttaat tcggatagat tatctggtgt aactaccaaa acgtttggta ctttaaaaaa
     8401 tggaattgta gatttatcta tttttttaga aagattttcg tatttagaaa tgtctatttc
     8461 attatttaag tcaacatcaa ctaaaacttg ctttatatta ccaaattggt ctgtatattc
     8521 aataaataaa tcgtatttca catcatctac actaacattt cggtaaaatc tttttacatc
     8581 acaacctgca gtattgattc taacaactaa ttctgatcct actagagcct ttattaaatc
     8641 atctccctta aaaagtttac taccgtttaa atagtataca taagagaggt ctttataatt
     8701 ataataatcc acatcacaag gatatttttt tagtgtatat tcactacaca tttttctcaa
     8761 agttttttta aaattttttt tattgtatct tttttggtct ccataaattt ttctaaattg
     8821 agactctgta acacatctaa ccctgctaat taaaagtaat atctcttttt ccatattcgt
     8881 catagtacat tctccttgaa gtattatttg caaagttgta ctaatattat attaatttta
     8941 tgacattaaa tttacaaaga attaaaataa aatagctaat ttttatagaa ttaaattcta
     9001 taaaaattag ctatcactat gttaaatact attatcaatc tttttattta aattatacat
     9061 taccttgatt ttcagaatct ttggaatctg tattttcatc agttcctgtt ttttcttttg
     9121 cttttttatc ttcaaagttt gcaatattta ggttatcaaa ttctgacatt tctattacac
     9181 cagattcttc ttttgaatac gatattttga ctttatcacc aacctgagtc aatggcaatt
     9241 cgctagatac ttttgaagta gccctaaata ttatatcttt atttgaatct aaagttacat
     9301 aataattagt gttaccacct cgcacatctg ggcttattct tgtcactgtg ccttctaata
     9361 cctcatcttt taaatcatta tcaagtttta aatcattacc cttagattct aatgcatcct
     9421 tatagtttct taatgcttca ttcttatctt cacctattcc aagtacatta taatcttcaa
     9481 cagatacaaa tgcaactctc ttaacaagac cagccttatc ttttaatgat gatacatatg
     9541 ttggctgacc caatatatta tacattacag ggaacgtcgc ttcataattt ttttcttgaa
     9601 cattaccttc agctgatttc attgcagccg tttcagtagc tcctggttgt ttatataatc
     9661 tagcttcttt tgttctagaa tcaactaaca taaatccaat tgtagattca tctccacctg
     9721 aagatgttat acctgtatac caataagacc tgttatcatt gccataaact agtgatgtac
     9781 cttctgttgg tactaaaact cccttttctg atataacaga gtttaaaaat ccattaacat
     9841 aaacacccca gtctttaatt tggtctgtta caaagctttg tggttgtatt ctatccaccc
     9901 atttaggcgt gtttttaaca tcataaacat ttatttttcc agtttctgca tcaacagtgg
     9961 ctattcctgt tgcatttgct cctccatagc caattttatg ttcatataaa cttacaaccc
    10021 aataaggtct tccttcatca tttatttcaa gagtgaaatc tgtcatacca acatttacaa
    10081 taccatgtat gtataaatgt ctttgtaaat cctgatgtaa gtaggcttct ggttggtaaa
    10141 ctatctttat tggctttcca tctatttctt gaactaattg gacgtcttga ggattactag
    10201 ctgatacttt tacataacca cttgtaccac ttagagatgt tatccattta attatgtctc
    10261 tgtgaacaag aggcgctacc cagtacaact ctccatctac attttgtata tgaaattttc
    10321 ctaatttgga tacactccct attgcaggaa cttctccaag ctttttgtca cctaatttca
    10381 ttgccatatc ttcatctaca agacgaatgt catttacact gacagggcta atgtcatcag
    10441 taaatttact ttcagttact tttcccaata gttctctata tgattttgca tgaagtacag
    10501 gtgtagaagt tataaaggga actactacaa tatacacaag caaagctatt gctattgaaa
    10561 aattatattt agccatttta gatgctcttt catttctatc taacatcata tctagtattc
    10621 ctgctacaac aaagaaaatt attcctactg aaaataaaga tgtaaaatct aatcttagta
    10681 caggtaattt aataaatgca taaattatta caattattaa tgcaactcca tatgttttaa
    10741 gatatcgttt catttaatac tcctctctaa aaaatgttat tattactatt atatccatat
    10801 atttacaaaa aataaatatt tatcatattt attatatatt aagatatctt tatatattta
    10861 tagttctgct tattcaacta tatttaacat tatacgtgta tgtataaata ctttttatac
    10921 actgtttgta ctatccatcc cgcacctaaa ggagtgggat ttttgcacta ttctgtatta
    10981 tatatatcca aaaatattat aaaaatacaa atattagcag aaatttaaaa actgaagaaa
    11041 gtattctaac taagatttac aaattcttaa tcctatcgaa gttaaactac tatcttcagc
    11101 ctaattatct agcaatattt ttttatatga aaaactctaa atcaagtttt taaatgtttg
    11161 cgatttttga tacataagaa tgttgtctag aattatattc tgataataaa ccttcatact
    11221 ctaaggttaa gccagataaa ttataaaaat catttggcgt tacttcaaat gcttgtttat
    11281 ttaaaatgtc tatctcactt ttttctaaca tgtcagcaac aaaatttgaa catacatatt
    11341 tatattcaga ttgtctctga atattcatta gtaaatatat taatgccatt gtatcgtaat
    11401 agtaatcttc tctataatca ctaattaatt ttatattttt atatagattt tcatattgta
    11461 aattatctac ttctaacgaa tataccctac acacagtgtt ttttcttata gcatataatc
    11521 cttgatttat attttcttca acaaatcctc caataaatgg attccttgga tttagccttc
    11581 caaaagaata cattggtttt agattttcat ttaatgatat tgacacatgt gtatacagct
    11641 ttttacttat attccttatt aagtaagata atatagttcc tgtatatgtt gttacaatat
    11701 agactttatt catcatttcc ccccaatcta gtaactaaat aatattatac acttacaatt
    11761 taatatcata agatattcta acataataaa tgtggtttta caaattttga ttttaaggta
    11821 ctttaatatt atataatata ataaaaatat aaatataagt tttctatatt tttaaccaac
    11881 agatgttttc ttcaaattaa tcagatgatt tagtatatct tgaacctttc cagagtcctt
    11941 taaatatcct aatgccccat ttatttgtga ttttaaatct atttctactt ccatatccaa
    12001 atcaacattt ccttttttta tagatatatt tttaagtgtt attggataaa gataactttt
    12061 ttccaaagta atcttatttc cattaacctc aaatggaact tttttattgt agttacttaa
    12121 tattttttct agcattttat cacttagttt aaattttcca attttcacat tctcaagact
    12181 tacaactaag tttccatcct ttaaagtagg tctcaattca agttcatatt gactatttac
    12241 caaaccaaat accttatatg gtcctgctac ctttatttta ccatttttca tttcaacaaa
    12301 attattttca agttcattaa ttccatgctt ctgcatcaat gtatatatta aatttttaaa
    12361 ctcttcctct gaaacagata tttttccaac cattctaagt ggatttttta ctacctccat
    12421 agaatttata tatgagcctt ttgataaata ttcttcatct atgattttat tattttgtga
    12481 actaacatca tatctttctt ttggtgtcaa gatatataca acaattccta caagtactaa
    12541 aacaataaca attaaactta acaaaatctt tgcaaccttt cccataatcg tctcctttac
    12601 aaatctgtca tcagatttaa aataaattta gataactaac ttcacataat attcttaaca
    12661 atatacatac tataatacag tcttttaaaa attaattcaa ccaatctaat gttttgtaaa
    12721 taaactgtaa aaacatgttt ttagtataaa agttaatatt ttgcataata ctcttaatta
    12781 cacctttatt tttttctaat cttggattaa aattaatatt atgcaataca gtgtagtgtt
    12841 ctgtattata atcaaattaa ataggaggta tagaatatgt caaatgaaac gaactcatct
    12901 aactttataa agaatattat tataaacgac ttagaaacag gaaaacatga cagtatcata
    12961 actcgttttc cacctgagcc aaacggatat ttacatattg gtcatgcaaa aagtatatgt
    13021 cttaattttg gattagctaa ggagtttaat ggaaaagcta atttaagatt tgatgataca
    13081 aacccattaa aagaggatgt tgaatatgta gagtccataa aagaagatgt aaagtggtta
    13141 ggatttgatt ggaacgagtt aaattttgca tctaactact ttgatgaaat gtataagaga
    13201 gctttaatac taataaaaaa gggtaaagct tatgtatgtg atttgacaca agaggaaatg
    13261 agagaatatc gtggtactct aactgaacca ggaaaagaaa gtcctcatag aaacagaact
    13321 attgaagaaa accttgattt atttgaaaga atgaaaaatg gtgaatttaa agatggtgaa
    13381 aaaacattaa gagccaaaat agatatgtct tctccaaata taaaccttag agacccaatt
    13441 atatatagaa tatctcattc aacacaccat aatacaggag ataaatggtg catatatcct
    13501 atgtatgctt ttgctcatcc aattgaagat gcaattgaag gaataactca ctctatatgt
    13561 actttagagt ttgaagacca aagacctctt tatgattggt ttgttaaaga atgtgagatg
    13621 gaaaatatac ctagacaaat agaatttgct agacttaata taaataatac tgtcatgagt
    13681 aaaaggaaat taaaacaact tgtagatgag ggtattgttg atggttggga tgaccctcgt
    13741 gttcctacaa tatccggaat cagacgtaag ggatatactg cagaagcatt gcgcaatttc
    13801 tgtagtgaaa taggggtatc aaaagttaac tctacggtag atagtcaaat gcttgattat
    13861 ttcctaagag aaaacttaca accaaaggct cctcttacta tgggagtttt aagaccttta
    13921 aaacttatta taactaatta cccagaagat aagattgaga tgcttgaaat agagaataat
    13981 gctaaagatg aatctcaagg aaagagatta gttcctttct ctagagaatt gtatatagaa
    14041 caagatgatt ttatggaaga acctgttaag aaatatttta gatttttccc aggaaatgaa
    14101 gtccgtttaa aaggtgctta ttttgtaaaa tgtactgatg taattaagga tgaaaatgga
    14161 aatgtagttg aaatacatgg tacttatgac cctgaaacta agagtggctc tggctttaca
    14221 ggacgtaagg taaaatctac aatacattgg gttgatgcta aatcagctat cccatgtgaa
    14281 tttagattat ttgaaccatt aattcttgat gatatacctg aaaatgaagg taaacatttc
    14341 ttagaacaaa taaatcctaa ttcattggaa atattacaag gttttgttga acctacacag
    14401 ataaaagatg ctaaaccttt tgataaattc caatttgtta gaaatggatt ctttagtata
    14461 gataataaat atacaactga tgaaaaattt gtatttaata gaatagttcc tctaaaaagt
    14521 tcttttaaac caggtaaata atcttacaat caaaaaatga agacttaaaa tataaattac
    14581 ttctactata aataaactta gttagaagta attttttatt tgagtcttca tcatttactt
    14641 taaatgatta acttttttca ctacagattc ccatgacttc tattccatct tttatgcatt
    14701 ctatgttaag aggaaaatct ggtccttttt ctccgtcaat atctgttata agtgaatctt
    14761 cacattctat atgcaccttt ttagttttaa agtatagtat ttcatttcca ttatattgat
    14821 ctaaataatc acctctaagc acacttatca aaactggtat tgattttggt attggcattc
    14881 ctttaaaaat aattacatcc aaatatccat catctatttc agccttatat gctagattta
    14941 tatttcctgc tgttttgcca ttaaaaatca acataagata catatctcca tcataataca
    15001 tttcctcaga agatactttt atcttaaatt tcctcatata caatgcttct tctattccct
    15061 ttatataata agaaattctt ccaaatgaat tttttaagtc tgtgtttatc ctttgtgata
    15121 catccgtaaa cataccagca cttgcaacat ttataaagta cttattattg actttcccta
    15181 tgtctatttt ctttggtttt gaatttatga tttcatttat agcttctttt atatcaaatg
    15241 aaatttgaag agcatttgaa aaatcatttg cagttcctgt aggcaaaatt ccaattggaa
    15301 tttttatgtc taattctttc attgcattta ctactaaatc tacagtacca tcaccaccag
    15361 ctatcaatat gtgttcataa gaactatcaa cttccttaaa tgcattcttt acaggttctt
    15421 taatagttaa tctatagggt atcaataaat aatttttttc ttgatataat tctatgatag
    15481 tgtctaaatt tgataatatc tttttttcac ctgaattagg attgtatata agttttactt
    15541 ttttcattat ttctccttac tcattttatt cttttagtat ccaaaatctt catttaaaaa
    15601 aatcacatgt attctttctg gtgttaattg tctttttata agtgtataac tacagcatat
    15661 tcttcctgga cttgaacagt ctatacatat accagtagtc ttacatccag tatttgtact
    15721 taatctttta caattggctg gagcagctat tgtttttgtt ctttttatag cttcatctac
    15781 atctttaact attttattta ctcctgctat aactattact ttatcaggtc catatatcat
    15841 agcagcaact ctattcccat tcccatctaa attgtaaagt tctccatcct ctgttatcgc
    15901 attagtactt gtaaaataag catctgcaga aaatgacttt ctatatattt ctttcatttc
    15961 ttctggtttg atatcttttg catatctatc aagaaaatta tatttttctc ttataaaatc
    16021 aatcactcct gtttcaaata aggtcataga acctccacat cctactagag aacctgcttt
    16081 aactatttct tcaatttttt taagaagctc ttctttgtta cttactaaat accctttcat
    16141 attattgttt tccaaagatt ttattgtttt ttctattctt ttttcattaa gccaatttaa
    16201 attttgatcc attttaactt cccccttatt tttaatatgt acttaattat ttataaactt
    16261 tattttcttt ataagtttat tttagtacat ctaaaaaaaa atacaatata aaacttaaat
    16321 agttttacat aattttttcc aaaataactt actcaatcaa aaaaggaaag tagatgtact
    16381 attctacttt ccctatattg tatgtagtat ttattgccta aaatctatat attaattata
    16441 tgtttcatat tatataatat cacagttaat taaaatgtaa tactatcttc tttttaaaca
    16501 tataaattgt attattaaat ttaatatatc caacttcaat aaatcttatt aatttgatga
    16561 agaagatgct atagctgcac ttgttgctgc cattacacat gcaatttgaa tagcaacaac
    16621 aatatttaaa aatatattat tagttataga gcctatatta ttctcatttc gtatcaaatc
    16681 tgcataaatt gaagctacaa tagcagaact aatcattact ctattacttt ttcctaaaga
    16741 ccaatttcca aagccttttt cttctttaag tttatttgaa acatttttta tctcttctat
    16801 tatttcatct tctctataat ctaataatga aattgctcct attattggat atccataata
    16861 attcatttta caatcatttt ctttaaatgc ttttttaagt tcaatagtct ttttacattt
    16921 aagcatagtt ctatcatcac taaataaaag aatttgggat aatgcttgta aatcatttcc
    16981 tttataaaaa ccattttcat ttagaaattc ataacatttt tctatgtatt ctaaatcttc
    17041 atctaaattt tttgaatgaa ttgcaataag tgctatatta caatagtcat cactagaagt
    17101 tagaaatgga tgttttttcc tcatgtaatc atatattatt ttcatttttg atatacatgt
    17161 aagataatcc attttctctc tattttcata aactattgat gctgtaagtg gtaaatatgt
    17221 atcgccccaa aacttttcac ttttaagttc tttgtatata tctaaaatca tcttaaaact
    17281 actttctgga tttgattcaa acgaaagtag tgttgccata taaaacatat tatatcctct
    17341 aaaattagag aataatccag tatttttttt aataagctct attcgttctt ccactaatct
    17401 aggacttatt ttcttgttct taagggtaaa tgcaagtgca caactatgtt gtatcaatcc
    17461 catttgccaa gaacctttaa cttcatttaa tgctttgtaa ttttctatta ataagtttac
    17521 ttttccattt ataaacgatt gttccatatt gccctcctag taatatatct cattgtacta
    17581 atagttttat atttattata tatatattaa atactacaat caatacatat tacttcaaag
    17641 aacatcattc ttcaacaata atgtagcctt ttttgtaatg tcttcccata ttattgactc
    17701 tatcatagta ctctgattca ctttcattac tccatttggg cacaaataca tctattgcat
    17761 tagaattact aaaatactta ctagaccccc tatccataac agtttttaat tttccatcta
    17821 caataatctt agttccaaaa cttaagtgat tgtttgctac aaaaccatct tctaaatcat
    17881 ctcctgtagc agttatcgct ccatatccat tttcttgatt aagacttgtg taataactaa
    17941 cttcaaaagt tataggtact aacttcttta ttttaatacc taactgattt tgtagtttct
    18001 ccttttgtac tttcaactta tactcatcac tatttttata atcttccata ccttgattat
    18061 acttggaatt aaagctttta cctgacttta ctatagtatc tatatttact atagattctt
    18121 tacaatgatc tgcatctatt tttgtttctc catttaccaa taatggagtt aaacatacac
    18181 tacatacaac acatgtaatc aatgcttctt taagcttcat aaattccccc cttattattt
    18241 tgatatatta gcatatttaa tattatatgt ctacatttag atttaaatac cacacccatt
    18301 tgctttaaga agttgtctta tatttaattc accttaaaca cttataaatc ctctatctta
    18361 gaatcatata gaataaagct tttatcatta aatattt
//
LOCUS       DAMJWS010000086        18391 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMJWS010000086 DAMJWS010000000
VERSION     DAMJWS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.087"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..838
                     /locus_tag="PC399_003006"
     CDS             <1..838
                     /locus_tag="PC399_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2593852.1"
                     /translation="FRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLKIKN
                     NSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFL
                     VNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDVYTD
                     SKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRVFDK
                     GFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDYLSI
                     DS"
     gene            1269..2429
                     /locus_tag="PC399_003007"
     CDS             1269..2429
                     /locus_tag="PC399_003007"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF2593853.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            2431..2829
                     /locus_tag="PC399_003008"
     CDS             2431..2829
                     /locus_tag="PC399_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593854.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            3091..3399
                     /locus_tag="PC399_003009"
     CDS             3091..3399
                     /locus_tag="PC399_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2593855.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            3488..4834
                     /locus_tag="PC399_003010"
     CDS             3488..4834
                     /locus_tag="PC399_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2593856.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPELLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            5067..6164
                     /locus_tag="PC399_003011"
     CDS             5067..6164
                     /locus_tag="PC399_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2593857.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKRINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDIGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            6263..7177
                     /gene="murQ"
                     /locus_tag="PC399_003012"
     CDS             6263..7177
                     /gene="murQ"
                     /locus_tag="PC399_003012"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF2593858.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            7287..8207
                     /locus_tag="PC399_003013"
     CDS             7287..8207
                     /locus_tag="PC399_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF2593859.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            8224..9075
                     /locus_tag="PC399_003014"
     CDS             8224..9075
                     /locus_tag="PC399_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2593860.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            9687..10748
                     /locus_tag="PC399_003015"
     CDS             9687..10748
                     /locus_tag="PC399_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2593861.1"
                     /translation="MKKIRKLTTKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(10864..11838)
                     /locus_tag="PC399_003016"
     CDS             complement(10864..11838)
                     /locus_tag="PC399_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593862.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQILRDNNFYK"
     gene            12294..13175
                     /locus_tag="PC399_003017"
     CDS             12294..13175
                     /locus_tag="PC399_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF2593863.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            13557..14360
                     /locus_tag="PC399_003018"
     CDS             13557..14360
                     /locus_tag="PC399_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593864.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            14372..15502
                     /locus_tag="PC399_003019"
     CDS             14372..15502
                     /locus_tag="PC399_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2593865.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            15506..16915
                     /locus_tag="PC399_003020"
     CDS             15506..16915
                     /locus_tag="PC399_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593866.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            17126..17635
                     /locus_tag="PC399_003021"
     CDS             17126..17635
                     /locus_tag="PC399_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2593867.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         7350 a         1888 c         3222 g         5931 t
ORIGIN      
        1 ttttagaaat agttacacaa atttatataa ttgtatagaa aatagtggtg aaatagatag
       61 ttatttagtt gatggacaat cttttgaaga aaatctaata aaagatatta tagaaaactt
      121 aaaaataaaa aataatagtg acatagagac ttataaacaa tcattaaaag aattggatga
      181 gtacatagct aaatgggttc atgaaataaa aattccaata tcttctttaa gtataattac
      241 agataggtta agtagcatag aagatagttt ggatattaag aatcaggttg caaagataaa
      301 ttttttagta aactctattt tgtatagtag tagaagcaac actatgtttg aagatgtatt
      361 tataaataaa tttaatttag aaaaattggt aaaaatgtct ataaaaaata attcattttt
      421 gcttataaaa aataatgtag aagttagttt gaatgaatta gaaaatgatg tttacacaga
      481 ttctaaatgc atgtcatatg ttttagacca aattattaat aatgctataa agtattcaaa
      541 ggaagttggt aagattgaat ttaattcaaa aaagttagaa aatggagtgg tattatctat
      601 aaaagatttt ggtataggta taaatgagga agatatatct agggtttttg acaagggatt
      661 tacggggaaa aatggaagaa atcaattgta taagtctact ggaatgggga tgtattttgt
      721 aaagaaaatg atagatagtt tagggcatga gattgaggta tgttcgaaaa ttggttcata
      781 tacaattttt aatatctact tttatgatat atcggattat ttaagcatag atagttaggt
      841 tattagacta taaaatctat catcatatac aaatcgacat atacaataag aataaattta
      901 gttcttgaaa atagtgaaga tttaattagt gccttaaatg atgctccatc tatattataa
      961 aataataatt attatatatg aaaaacatat cagataggta cctggtatgt tttttatttg
     1021 tagtaactat tttaatatgg tttttggaga gagaatatgt tttgtttgtg aaaaaaaatt
     1081 acatggtatt acattgacac tgaaaataaa ttaatgtaag atttagctaa ggtaaaaaac
     1141 gtgtataaga ttaactcgat taatttcgta agagtaaatt atcgtaatta aagatgaaat
     1201 aagattaatt ctaaaattat taaacttaat accttgattt ggggaggaat aactataaac
     1261 ttaaaaggat gataactatg tatagtgtag gtttaatgtc aggaacttcc ttagatggag
     1321 ttgatgcagt actagctgaa attagtggga atggtagaaa tactaaagtt aagcaaatag
     1381 aatttataac tctagaaatt cctaaagata taaaagacga aataagaaag tgctgtatag
     1441 aagaggaatc gagtgttgat ttaatctgta gcttaaattt taagcttgga tatttattta
     1501 gtaaagctgt aaaatcagtg tgtcataaag caaactttca tatagcaaat ttggatttta
     1561 tcgcatctca tggtcaaaca atctttcata taccaagaag ttataataat tttgtatctt
     1621 caacgcttca aataggtgaa ccagcagtta tagcatatga aacaaataca aaggtaatat
     1681 ctaattttag agtaatggat atagcagcag gtggagaagg tgctccatta gtaccatata
     1741 gtgaattttt attatatagt gataacaata aaaatttagc acttcaaaat attggtggaa
     1801 taggaaatat tactataata cctaaatcat gtaacataga tgatgtattt gcatttgata
     1861 caggaccagg aaatatgata atagatggag tatgtcaaag attatttaat agaaagtatg
     1921 ataaaaatgg atactttgca tcaaaaggta aaattaatga agaaatgtta aaagatttga
     1981 tgagtcataa gtatcttagt caagcgccac ctaaaagcac tggaagagag gtatttggac
     2041 aagtttattt ggataacatg cttaacaaat ataaacatgt agataaatat gatttgatag
     2101 caacagtaac tatgtttacg gcaaaagcta tatattataa ttataaaaac ttcatacttc
     2161 caaaggttaa tgtagatact ttattaattg gaggtggagg agctcataat ctaactttaa
     2221 taggatatat aaaagagtta ttattagagg ttgaagtatt aactcaagat gaatatgggt
     2281 attcttctga tgcaaaggag gctttggcat ttgtaatctt gggcaatgaa acattaaata
     2341 attctttttc taatgtaata tctgcaacag gggctaaaaa taaagttatt ttagggaata
     2401 taacacctaa accttttgga ggtaaatgac atgtctaata ataaaaaaaa gaaaagaaag
     2461 aaaaataata attataaaaa tgcaaaaagt acaaaaagta ataaaaatac taagaaacaa
     2521 atggagaaaa atcaaagaac agattataat attccaacta taagttttaa aagtggatta
     2581 atactaatag tttcatctct tgtaagtact gtagtttttc ctttgttatt atctatattt
     2641 ggaatagata atagatttgg agttgttata ggaaattcat taattacatc ttttgcagtt
     2701 gcatatacaa gatactttgt tgaaactaag agaggttttt ctttgggatt ttttagactg
     2761 tatattttat ttgctttatc atttgcaata ataggttact tctggatata taaaggaata
     2821 catatttaaa aaagtattga gactatttta tgttaataat aagatagtct tttttctttt
     2881 ttaagaaaag atatgagtat tttaagtagt gtgtaatttt agacaaaaga atgattagat
     2941 aaataaagca attaaagcaa gatataaatg catgaaataa aattacaatt taaaatgtaa
     3001 aatatgaaaa aatatataca aacgtattcc tgtaatcaaa aatacatgct ataatgattg
     3061 aaaagaaata aaaaaacagg gggaatcaat atgagagtat tatttgtttg ttcatcaggg
     3121 atgtcaagtg ctatagcagt taatgcatta caaaaagaag gagcaaaaaa tggaatagat
     3181 atagatgttt tggctgttgg tactcaagag tttgagaatg aagttaaaaa tggatgggat
     3241 attgctatgg tagctccaca agtcagacat agatttgact atttaaaagc ttttgctgat
     3301 gaggttagtg taccatgtgc attaatacaa gcacaagctt atagtccatt aggaggacca
     3361 aagttattaa aacaagttca agaactgttg agtaaatagt agttgcagtg ttgttagatt
     3421 agaagaaagt tgattttata aaaattttaa tgataataaa aataaatatt aattaaggag
     3481 agaaaagatg ctagataaat ttacagaatt tttagacact aaattatcaa cttctatggc
     3541 cagattggca gaacaacgtc acttaagagc tgtaagagat ggaatcgtag cttcattacc
     3601 acttataata gttgggtcat ttttcttgat attagcattt cctccattac cagattcatg
     3661 gggggtaact cagtatctaa aggaaaatgc agttacaata ttactaccat atagaatgac
     3721 attttttatt atatcagtat attctacatt tggcataggt tatagtttgg caaagtcata
     3781 taagttagat ggattgacag gaggtttatt agctacagct acattccttt tgacaatagt
     3841 tcctactgca atagagggtc ttacccctga gttattaaag atagttgaaa agagtccaga
     3901 acttttagct tggtataatt caattcctca aggatggcaa atgccaatgg ctaatcttgg
     3961 tggaggttca atgtttgttg ctattgtaac atctataata ggtgttgaga tttatagatt
     4021 tactgacaaa tctaaattca agataaccat gccagaagca gttccagcat cagtcacaag
     4081 gtcttttgaa gctctgacac cagccgcatt aataataatt ttaatggcta ttgttactta
     4141 tttcctaggg tttgattggc atggcttcat agctaaatta gtaactccat tagtttcagc
     4201 cactgacaca ttaccatcag taattatact aatattttta ataacgtttt tttggtcatt
     4261 tggaatacat ggtgtatcaa taataggttc attggcaaga cctttatgga tacaattatt
     4321 agatgctaat acagcttcat atgctgcggg tcaagcagtt ccaggtattg ctgctgaacc
     4381 cttttttcaa tggtttatat ggattggagg ttctggagct acaataggct tagctttact
     4441 tcttgcattt agagcaaaat cagcttatgg aaagtcttta ggaaaagcaa ctgtaatacc
     4501 agcaatattc aatataaatg aaccaataat atttggagca ccaatagtat taaacccaat
     4561 attaataata ccatttgttg tagcaccaat agtaatggca acagtttcat ggtttgctat
     4621 gagtgctaat ttagtatcca gagtagtagt tactgctcct tggacattac caggaccagt
     4681 aggggcatat ttagccactg gtggagattg gagagcttca gttcttaact tattatgtat
     4741 agctatatca ataataattt actatccatt tgtcaagatg tacgataaga gattgcttca
     4801 tgaagaaaaa gttgaagagc aagaagctta ttaattagaa actatattgg aaattaaaaa
     4861 ctatattaga ttagtataaa tttcaaagta taacagttat gaaataagta acgaagatat
     4921 tttgttgatt gtttataaaa aaagaaaagg taaacttttc tttttttata aatatatatg
     4981 aaactttttt tcaccaaatt tttaaaaaag tttcgtgtaa ttcgaaataa atttaaacac
     5041 atataggata taaggagatg acacagatgg gaagattagg aatatcaatt tatccagaaa
     5101 aaacaacatc taaggaaata tatgattata tagataaagc tgctgaaaat ggatttacac
     5161 gtatattttc atgtttatta tcagttaaag atactaaaga aaatataatt aaaaaattta
     5221 aacgtataaa tgaatacgct aagtttaaag gttttgaggt aatacttgat gtaaatccca
     5281 gagtatttgg tgaattagaa ataagctaca aagatttgac tttttttaaa aagacgaagg
     5341 cagacggaat aaggcttgat ataggattta caggttctga agaggcatta atgactttta
     5401 atcctcaaaa tcttaaaata gaaatcaata tgagtaataa tactaattat atagatacaa
     5461 taatggacta ccaaccaaat aaagaaaact tgacaggatg tcataacttt tatccacata
     5521 gatatagtgg attaaattta gaacacttta taaaatgtac agaaagattt aataagtatg
     5581 gattaagaac agcagttttt gtaacaagtc aaaatgaaac tacatttgga ccatggcctg
     5641 taactgatgg gcttccaaca attgagatgc acagaaatct acctatagat attcaagtaa
     5701 agcatataat agctatgaat aatataaatg atataataat atcaaattgt tatccaacat
     5761 atgaggagct aaaggtatta ggttctatga gaaaagatat ggttacattt gatgtagcaa
     5821 tagaggaaaa tatacgagaa gttgagaaga agatactctt tgaagaaata cattttaata
     5881 gaggggacat atctgacagt ctgataaggt ctactcaatc tcgtgttaaa tataaaggat
     5941 acaaatttga actatttaat actcctaaaa taataaaaag aggagatata gtaattgaaa
     6001 gtagtgaata tggtcattat gcaggggagt tacaaatagc cttgactgat atgaaaaaca
     6061 gtggtaaatc gaatgtagta ggatatataa gaaaagaaga attatttata ttagattata
     6121 ttaaaccatg gcaaaagttt aattttaaac cctcaacaaa ataatttatt atgaaaatat
     6181 taatttatta taaaaatata attatgaaag ttggaattaa aatgtgttta aaaatttaat
     6241 attaagaaaa tgaggaaatg atatgttaat aaaaacttta gaaaatttag ttacagaagg
     6301 tagaaataag aatacattgc aaatagataa agaagataca ttaggcataa tagaattaat
     6361 aaataacgaa gataaaactg tagcctatgc tgttgaagaa caaaaggaga gtatagcaaa
     6421 agctgttaat ataatagtag atagaatgaa gcagggaggt agattattct atataggagc
     6481 aggcaccagt ggtaggatag gaatactaga tgccacagaa tgtcctccaa cttacggagt
     6541 ggattttgag cttgttcaag ctataatagc tggtggaaat caagcaatat ttaaagcaat
     6601 agaaggtgct gaagatgata aagaacttgg aaagcaagat ataatagata gaggagttac
     6661 atctaaggat gttatatgtg gtatagctgc atcaggaagg actccttatg ttataggtgc
     6721 aatggagtat gctaaggaat tggggtgtgc agtactatca ataactatga atccgaatag
     6781 tgaaatgagt aaaaaagcgg atttaccaat aaacattata gtaggggcag aagtaataat
     6841 gggttcaact agaatgaaaa gtggtacagc tcaaaaaatg gtatgtaata tgttaacaac
     6901 agcttctatg gttaagatgg gaaaagtata ctcaaacttg atggtagatg ttaagacatc
     6961 aaatgaaaag ttggttgaaa gagcaaaaag aataataatg atagctacaa atgtaaaata
     7021 tgatgttgca gaaaaattct tagaagaagc agataatagc gttaaattgg ctatatttat
     7081 gataaaaagt ggtttagata aggatagtgc aaagagtatc ctagatagac aagaaggtta
     7141 tataagtgaa gctttaaaat caatagaaaa attgtaaaat aaataatagt actgataacc
     7201 tttcaatatt aaaaaaacag cattggaagg ttatttttag atattaagta ttgaaagaat
     7261 tattaaagat attgtaggag gatagtatgt attttttagg tgtagatggt ggaggaacga
     7321 aaacaacatt tacattagta gatgaagaat taaatatagt aggaaccata accaaaggaa
     7381 cttgtcatta taatcaaata ggatttgata atctaacgaa attattaata actgggttgg
     7441 aagaagtttg taaagatgct aagataaatg tagaagaaat aacgtatgca tttgttggac
     7501 ttgctggata tggaaaaata aaagaagtat tgtatgcatt agaagtagca acaaaaaatg
     7561 catatagtca tataaattat actctaggta atgatgtaga aatagcatta gcaggttctt
     7621 taaatgggga aaaaggtata aatataattg caggtactgg ttcaatagca caggctttag
     7681 ataaagatgg gaatcttcac agatgtggag gatggggcta tgtattagga gatgaaggtt
     7741 cagcgtatta tataggaatg gctaccttaa aaatgttcac aatgcagtct gatggaaggt
     7801 gttcaaaaac aaaattatat gatttaataa aaagacatct taatatagag aatgattatg
     7861 acataataaa atatgtaaat gatgaaatac aaggagatag aatagaaata gcaaagtttg
     7921 ctacgatatg tttaaaagca gttattgaag gtgataatac tgctagtaaa atatttgatg
     7981 atgcagctta tgaattaagt aggcttataa taggattaga gcatcatttt gaacaaggta
     8041 caaagataaa ggtatcttat tcaggtggag tgtttaagtc aggagattta atattagagc
     8101 cacttaaaaa atatttgaat aaaacgagat ttggtatagt gagaccagtg ttgactccag
     8161 atttaggagc atgtttactt gctaagaaga aatattatag taaataggta gaggtgatag
     8221 agtatgagtt gcattttaaa attaaaacag atatatgaag atttaacgga agtagataaa
     8281 aaaatagctg attacatact taataataca gaggctataa gtaagctttc tgtatcagaa
     8341 ttggcgtcaa actcaaaaac aagtacagca agtatagtta ggttttctag aaaaatggga
     8401 tattcgggtt ttggggattt aaaaattgag atagctaaag atttgatggg caaagaaaat
     8461 gaatacacat atgtagatga aaactatgat tgtaatatag atagtgttat aaacaaaatt
     8521 acaaacaaaa acatagagac tatcaaccaa acaaggttgt tgaatgaaaa agatataata
     8581 aaagaagcag ttgaacagat aataaaggct aaaaatgtat atatatttgg agtaggtggt
     8641 tcggctttag ttgcactaga tttacagatg aaacttttaa ggataaataa acaggcattt
     8701 acctctttag atagtcatac acaacttatg gtatcttcaa atgtagataa agaagatata
     8761 gctatagcaa tatcttactc tggagaatca aaagaagtaa taaagtctat agaaaatgca
     8821 aaattaaaag gatgtaaggt catttgtata actaaatata gtgacaatca tcttagtaag
     8881 attagtgatt taaagctagt agtaccaaat atagaaaaaa gacttagaga aggagctatt
     8941 tcttcaagaa tagcaatgct tacgttgata gatataatat atatttctat aattcaagaa
     9001 aacttaaatg aggcagaaca gaaactacaa gaaacaaaaa ggatacttga ttatttaaat
     9061 attaataatg cttaaaacta ggttatatat caactaaaca aataagttag gctataagcg
     9121 taagtcaata agaaatagat aaatatctaa aatattgact tacttaataa taatatatag
     9181 catttttata atacaaatga gttgtcttaa aattgatttt ttttaatcaa attttaagtt
     9241 caactccttt tttatatttt tatagatata tcctatattt tcccagaaaa cgtattgaat
     9301 atacaaatta taagaagtat aatgttgttg ggagagaatg tatgaaatgt atacaaagtt
     9361 gattttaact gtattattga taattctctt tactcttacc attattaatg attgaagaca
     9421 atggtattta ttataggtca aagattccga atgtattaat catcatcatt ttctcaaact
     9481 aacagcaaat accctgattt gcgagttgta ttgtatacaa attatctaga aaatatctat
     9541 tagattttcc ataaaattta aatatttcac gaatgtatga atggagaaat atacatgttg
     9601 acaatccacc tttttaaata ctaaattaat tttattaatg gtagagaata ggaagtaaca
     9661 atgttttata aaggagaata ttaagtatga agaaaattag gaagttaact accaagttat
     9721 tgattacaac aatagtttta atctcaggta tgagtatgac agtttatggt atgacagcca
     9781 aagaggtaac agcaaaaaca ccaaaaagct atgtcactgg aaccaatagc gtatatggtc
     9841 caaagctgtc acaagcccaa ctaaactcag tagcacaagc aacagcagat tttatgaaca
     9901 agaaaatcac aaagaatatg accacagatg ctaagatttt agttgcctac aatcacatta
     9961 aaaataacac aacatatgtt gactggaatg cagtagaggg agcaaatact gcctatacat
    10021 tggttactaa aaaaggtgca tgctcaggaa tggctaggag catgaaagct ttgtgtgatg
    10081 ctatgggaat tgaatcttat tatgtacaca gcacatcaaa tgaccaccag tggaatctta
    10141 tccgttttgg tgatgggcgt ttgtatcatg tagatattga tgctaataaa tcagcaggaa
    10201 aggatatcat atacaagagt cttagccacc cattaccatt tgataagaca gcatatccaa
    10261 aagttggtgc aatagtacag acggtaaaac cagaacagac acagaaacca gcaccacctg
    10321 gaaagcacac tatgttacca catcaagaag gggcaaaggc tgttggaaat tttggaaaaa
    10381 aaggagaaga tataaatgaa aaacatgtaa tgtatgaagg tgttagatgt agtgtagtta
    10441 attcatcagt aaatacaatt acatttacag gtagtgaatc aaatttagac ccatcaaatc
    10501 ttacgattgc tgtaatagga tttatgaaca caccatatgg gatgtttgca ttagcaccag
    10561 agggtaaacc tgttgaatat ccttttaaat atgataagcc attttcttta gattttagtg
    10621 gtaaaaatcg ttctggtgca gaattagcga agtactgtaa aaaaaatgat gtgactatta
    10681 aaatagaaat cactgatgaa aaagctaata ttggacttgg tcaaataatg tatgttcgat
    10741 atgaataatg atttaatcat gacagtagaa taagttataa acattgcgcc ctcttactag
    10801 gggcgcattt aatattatat ctgatatttt gtagtcaggt caactgctag atttctcccc
    10861 tttttattta taaaaattat tatctctaag tatctgagca tgtttacgat taactaaaaa
    10921 ctgttttaac tcagtcaagc ataggaataa aatagctcta ttttcaaact ctgttctgtc
    10981 aacaggcaga ggctggtttt tcatactaag cattagtctc tttaattctt caagaagaag
    11041 attgcctgtt tcagcttcaa attcagtata acctatctta tgtataaaag cagatagaat
    11101 atgagcttgt ggtggtgtag aatttagttg agatacataa ctatacaaat tttctaagat
    11161 atcccgttgg ctttttataa tatccatatg ttctaagaag aatctagtat cacttaataa
    11221 agtattatta atattatcgt aagtttcaga aatactgctg tcaataagtg aatttaatgt
    11281 atataaatct ctactatatc cattttcttt tttaggattt acaataatat ctgcaatgtc
    11341 tataagtaca atactcactt cttcctgtaa ctttttttga acttcatgga ttttatgtat
    11401 atttgttgga atatacatat ttattaagac acctattcca gttcctactg caaatattcc
    11461 agcttcattt aaaatccatt tgatagaagt ttctccttga aggtagtaat gggatgctat
    11521 gactacacac atagcaataa catcttgaat atttaacaaa aaacaaagtg gtataattat
    11581 aaatataaat attgaaaatg aagtcaaatt atatcctaaa atattaaaac ataaatatga
    11641 aaaaatagta cataaaagaa aaccataaat ctttccaatg gagcctttaa gagtttctct
    11701 tttagtgtcc tttactgtaa gtagtactat aactccagca accattgaat attctaattt
    11761 taatagccaa gcaataaata tagcaagaga acatccaaga gataatttta ttactttaag
    11821 tgaattaata ttattcaaat gcaaacctcc tgtaaatcaa tataataaaa ttctaacata
    11881 aaactaatat tgcttgaaag gtaagtaact atacttaatt attttattgc cctatttgtt
    11941 tagatacaat caatataatt ttattttatg aaaatttttt gtaaaatttt aaatttagag
    12001 actaacatga gaccacaaag aggaagttta ttataagaag aaaacttaaa attgatttta
    12061 aatagcaaat gaaaataatc aaatatgtat atactattat taaaaaataa aattatatag
    12121 ttaatataat tttataattg gtttagtata gagattttta acttttatgt tatactattt
    12181 ataaaagtag gtataaagat taaaatacat aatcaaatat gtataagagg ttttaaatag
    12241 tattgttaat gaacttgatt attgtttata attacaaatt ggaggagata aacatgaaaa
    12301 ttactgaagg atatatgcct tttaaaggtt ttaaaacgta ttaccgtata gtaggagaaa
    12361 atacggaagg gaagaagcca ttagtattac ttcatggagg tccaggttct acacacaact
    12421 attttgaggt attggataag atagctgaaa gtggtagaca agtaataatg tatgaccaaa
    12481 ttggttgtgg aaattcattt gtagaagggc atcctgagct atttaatgca gatacttgga
    12541 tagaggagct tattgaacta agaaaacatc taggacttga tgaaattcat ctgttaggtc
    12601 aatcttgggg aggtatgcag gctatttggt atgctattga atataaacca aaaggtatta
    12661 agtcttacat cctttcatct actctttctt ctgcaaaact ttgggagaaa gaacaaaaaa
    12721 gaagaatatc atatatgagt gaggttgacc aaaaagcatt acttgatgca gtaaatactg
    12781 gagattactc tagcaaagaa tataatgatg cattagaaag atttatggaa atgtattgtg
    12841 caggtgaagt gacagaagat tctccagagt gcctgagaag acctaaaaaa tcaggttctg
    12901 aagcatatat tgtaggatgg ggacaaaatg aattttctcc tacaggaact ctttctggat
    12961 atgaatttac agatagatta catgaaataa aagagccttg tctagtaact agtggagcaa
    13021 tagatttatg ttcaccatat attgcaaaga ctatgtatga tagaatacct aatagtaagt
    13081 gggaattatt tgagtattca agacatatgc cattcgttga agaaaatgag aaatatataa
    13141 aagtcttaac tgagtggttg aatgcgaacg attaaaaact atataaactt atataaaaaa
    13201 atgagtgtct caaaatgaac tttttagttc attaggcact ctttttgcat gaaatcaaat
    13261 atattttcat tatttgaacg ataaaatgag gcttatatct actttgggaa agtaattttt
    13321 tattactagc gttagattct aatctaattg tatacgtatt gaaataatag aaatatattg
    13381 gtgtttataa cttaatttta ttgtttttta gaaatttttt tatatattgg tataattatt
    13441 ctgtttaaat ttgattaaaa agtatattaa ttataataag aaacaattat ataaatagga
    13501 atagattatt ataattaata tagatataaa aataattata gaaagaaagg cacattatgg
    13561 aaaaattgag agttattgaa atgataaaaa ataatcccaa tattatagct acaatagata
    13621 atccaactga tgaaatgaaa ttgttagcta ttaaagaaaa tgggcttgta ctagaatata
    13681 taaataatcc aaccagagag atgcaagaac tagcaataga taataatatt agagcaatta
    13741 aatttataga taatccaaca gaagatatga tgataaaagc agtaaatgag ggatggagta
    13801 tactggatta tatcaaaaac ccaactgata aagttattga aatggctata aatcaagcag
    13861 gatgggcaat taaatatgct aaaaatccaa gtgaagaatt acaactgtta gcagtaagaa
    13921 aaaattatga ttcgataaag tttataaaac aaccttatga aagtgtacaa gaggaggcag
    13981 tgagaataag ttatgatgcc ttgagatata tagattcacc tagttataat gcagaactta
    14041 tagctataaa aaataatgaa gcagctatta gctttataac tgatttagat aaaaataaaa
    14101 tgttggaatt tttaaaagtg aatattttag tgattaagta cattctgaaa aaagtatcta
    14161 aagatgaatt agagagtgta ttaaaagaag tattatccaa agaagatgtt gaggaaaagt
    14221 atattagaga ttttttgaat tgtagtgtta tagatagaaa tagcgaaagt ataccaatag
    14281 ataaaattat gtttatttat aaatatggaa gtaagacagc aaaaaaaata gctgtagatg
    14341 agaaattaaa gattatgtag gagagagaaa aatgaatttt atagacacat taaaaagcgt
    14401 tgacttaata ttatccactg gagaagtacc tttaatagta ggtgaaagtg gaataggaaa
    14461 aacagcttta gcaaataagc ttgccaaaga aaatgattgg agtttaattg ttattgatgg
    14521 aaacttactt aaagagggtg aaataggtgg tcttccaact atagaatctt atgtaggagt
    14581 taattctaat ggatataaga ctgaaaagaa aacaacagtg tatgctgttc ataacaaatt
    14641 aagagaaatt gatgaagaga tatctaaaag taagacagtt cttttattta tagatgagat
    14701 aaatcgttgt gagcatacag tacagcaaga acttatgaat ttaattttaa atagagaaat
    14761 taatggatat aaactgcatg atgatgtaaa gattttagca gcaatgaatc catcaagtaa
    14821 atatggttca gattttgatt atcaagtggt agatatggat gcagcccaag aaaataggtt
    14881 tgtttggtta aatatggaga gtgaccatac acaatggata aaatgggcaa tagatgaggg
    14941 tattgaaaga aaagttatcg agtttatttc cacttttcca gaatatctgc ataaaataaa
    15001 tgaagatgat gtaagagcca ctcctagaag ttatgagaga gtatctaaaa tttataaagt
    15061 ttataaagaa aaaaataatt ctattcctag agcagtattt ttaaatgttg taaagggtaa
    15121 tgtaggtaaa gttattgcag aagaatttat aagttttatt gaatcaaact ctgaaccatt
    15181 aatatcctat gaagatgttt ttttaggaga atctattgat gagtctattg tagaaagagt
    15241 aaaaaatgag agtcatacaa gactttatct atcagctatg aacattctaa aagacttaga
    15301 attaaatatt aagaatgata aatatgaatc aaaccactat attaatagat ttattgaatt
    15361 tttaaaaatg tatcctgtag atttaatgat aggtattatg aaggatatta gaaacagtta
    15421 tattgaagta tataaaaaag ctatagaaaa tgaagagttt gtaaaatcat attttgaatc
    15481 ttatagttta ataaggggat aatgtatgga aaattatttt gataaacaag caaaagagct
    15541 ttataataaa gcaagtgaaa ttataaatac tcattctatg ctaaaggcaa atcgtgctaa
    15601 tgagaaattt gatatagata taccacaaga ttttaagtat gagtttttta gtcttgtaga
    15661 taaagtgaat ttaagtctta tggaagaaga agataatttt tatggatatt ttttgtttca
    15721 gatgtcaaga gaaataagat ttgatataag tagtcctaca ggtgtgaatt ttaagggagc
    15781 taaatatgtt atatatttta atccaataat ctttttaacg cttgacataa agcaaatgga
    15841 gactacaatt aaacatgaaa ttcatcatat attatctatg catttgatga gagcaaagga
    15901 gttaaaagga aagtatagta cattagcaat taatatggca atggatatag tagtaaataa
    15961 atttctaaac aatttaccac catatgcaac taccttagag tgggtaaatt taaagtattc
    16021 cttaaaactt gaaccatatg aaccttttga atattatgta gaaaagattc agactgaatt
    16081 agatttgatg gaagtggatg aagatggtga ggaagatgat agtgataaat atggagatat
    16141 agaaattgat tatagccccg aaagaactca tgatatatgg gatgaatcca atgagataga
    16201 tgaaaatacc cttagagaat ttactgaaaa atttattaac agttctcaaa aaggtaaagt
    16261 tcctgcttat ttagaaagta tgatatcatc acttaaaaat agtaagggtg aattgccatg
    16321 gaatttatat cttaatagaa taatgggaac agttgaaaat aataaaaaga agactataac
    16381 aagaagaaat agaagacaac caaatcgttt agatttgagg ggagaactta gaagtcataa
    16441 gtcagaaata gcagttgcac ttgatgtaag tggaagtatt agtgatgaag agtttaaaca
    16501 agctattaaa gaagttctta gtatagtgaa aaactataat catgaaatta caattataga
    16561 atgtgataat gaaattagac gtgtatataa agtgagaact gaaaaagata taaaagatag
    16621 aattaggata ggtggaggta ctagattttc tccagtcttt gagtatgcta ataataaaaa
    16681 gattaatttg ttagtctact ttactgatgg taaaggtgaa aataaactac aagtaatacc
    16741 tagagggtat aaaattttat gggttatttc tggaagagga gataaaattt cattaaaaga
    16801 accttatgga gcagttaaaa aacttactaa ggttaaaaca aatgatgaaa tattagaaat
    16861 aaatgatgta agaaatgatg ggtactcaat gaatagccaa gcaccaattt tataggtatt
    16921 cacaggagat aagctgtatt aatttatttt gatatgcttg ttataaaaat ctaaaaaata
    16981 cattaatcaa tggttaatgt attttttaat tttaataata taagttttac ttacatttaa
    17041 gtcttaaaat gtgttaaaat ataatatata gcagatagtt ttggcaaggt ggatttagtt
    17101 gtcaaaacta ttttttttat taaggatggt gatgtttttg tttaagattg atgattatat
    17161 aatgtatggt atgacaggag tctgcaaagt catggatata acaaatgaac gatttacaaa
    17221 tggaataaaa aaagagtatt atgtattgag tcctatttat tctaataata cagtaataaa
    17281 aattcctgta gataatgaaa aagtaccaat gagaaagtta ctttctaaaa ttaatgtgct
    17341 ttcattaata aatgatattc ctaatatgga tacatcatgg atagataatg aaaaattgag
    17401 aagtgagcaa tttaaaaaaa tactcagagg tggtaaatgt gaagagttaa taaaattagt
    17461 aagaagtata gataataaca gagagtatgt aaaatctatt ggtaaaaaaa cacatcaagc
    17521 agatgataat ataatgaaag aagctgaaag gttattaaat gaagaatttg caactatttt
    17581 agatatttct cctaatgaag ttagttcata tatttcaagt catatacctc aataaagtgt
    17641 tttagtatcc tttatttaat ctattttaag tattatttat gtatgctggt tttggtaaaa
    17701 taaaatatat aattaaatta tataaaaaat aatgatgatt gtattgaaga attacagaat
    17761 agtgcggaga gcctactcct ttaggtgtga gatggatatc acttaaagat ttaaattgtt
    17821 attgtagatg tttttaatat aaaacaagat taataagtgt attgtaagct ttaagtgaaa
    17881 ttgatactat aatgagttac ttagatttta aaatatataa tattataaaa aatcctttta
    17941 ggttttgaat gaataagatg gatactatgt tgaatagaat ccatcttatt taagttttat
    18001 ttatgttgtt tatttgtaag cattattttt gtacatgaaa acacagtgta aaaatatgct
    18061 tagaattatg aagttttatt aaaaagatat tcgacaaaat aagactaaaa atattcttaa
    18121 acttataact aagataaaga ttgtatattt atagtgtagc ctgaaaaatt gtggatgtac
    18181 gaaatttatt tagtatttta ttgaaacaaa gtagaagaag tactatagtt tatctttgaa
    18241 tagattgtta ttttttagtg aaaaagttat taggttaata cataaatata atagcttata
    18301 aatagtttaa gcatgttaaa aaagtagtta ttagagatat atagttacat tgcttataag
    18361 atacttaaaa tgatagcatt cacttatatt t
//
LOCUS       DAMJWS010000087        17824 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMJWS010000087 DAMJWS010000000
VERSION     DAMJWS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.088"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            14..643
                     /locus_tag="PC399_003022"
     CDS             14..643
                     /locus_tag="PC399_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF2593868.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            597..1025
                     /gene="ytfJ"
                     /locus_tag="PC399_003023"
     CDS             597..1025
                     /gene="ytfJ"
                     /locus_tag="PC399_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF2593869.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1273..1713
                     /locus_tag="PC399_003024"
     CDS             1273..1713
                     /locus_tag="PC399_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2593870.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1729..3180
                     /locus_tag="PC399_003025"
     CDS             1729..3180
                     /locus_tag="PC399_003025"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF2593871.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKIKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3271..3939)
                     /locus_tag="PC399_003026"
     CDS             complement(3271..3939)
                     /locus_tag="PC399_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF2593872.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            4208..4348
                     /locus_tag="PC399_003027"
     CDS             4208..4348
                     /locus_tag="PC399_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593873.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(4523..4843)
                     /locus_tag="PC399_003028"
     CDS             complement(4523..4843)
                     /locus_tag="PC399_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2593874.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(4963..5700)
                     /locus_tag="PC399_003029"
     CDS             complement(4963..5700)
                     /locus_tag="PC399_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF2593875.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            5915..7738
                     /gene="acd"
                     /locus_tag="PC399_003030"
     CDS             5915..7738
                     /gene="acd"
                     /locus_tag="PC399_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF2593876.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            7953..12251
                     /locus_tag="PC399_003031"
     CDS             7953..12251
                     /locus_tag="PC399_003031"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF2593877.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            12455..12916
                     /locus_tag="PC399_003032"
     CDS             12455..12916
                     /locus_tag="PC399_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF2593878.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            12946..14100
                     /gene="nusA"
                     /locus_tag="PC399_003033"
     CDS             12946..14100
                     /gene="nusA"
                     /locus_tag="PC399_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF2593879.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            14107..14388
                     /locus_tag="PC399_003034"
     CDS             14107..14388
                     /locus_tag="PC399_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF2593880.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            14378..14689
                     /locus_tag="PC399_003035"
     CDS             14378..14689
                     /locus_tag="PC399_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF2593881.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            14711..16651
                     /gene="infB"
                     /locus_tag="PC399_003036"
     CDS             14711..16651
                     /gene="infB"
                     /locus_tag="PC399_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF2593882.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            16694..17074
                     /gene="rbfA"
                     /locus_tag="PC399_003037"
     CDS             16694..17074
                     /gene="rbfA"
                     /locus_tag="PC399_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF2593883.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            17055..17732
                     /locus_tag="PC399_003038"
                     /pseudo
     CDS             17055..17732
                     /locus_tag="PC399_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         7190 a         1930 c         3268 g         5436 t
ORIGIN      
        1 tgataatata aacatggagc ttaatgaatt atttaatttt aagtatttaa taatagctgt
       61 aatttttatt ataagtgtaa ctatgttgat tttaatatct catataaaca tcttaataat
      121 agctgatata aaaaacaaag atatttgttt aaagcttaat ataaaataca tgtttaattt
      181 aataaatata aatagacaac tttatccagc tgaaaatagt aaaaataatg ataaaaaaga
      241 ggggatgaag aataatatag attcaagtat attattagct gatgaccttt tgagcattta
      301 tagattatta aaaaagataa aaatacatga actttattca aatattaatt ttggaactgg
      361 aaatattggc ttaacatctt ctgtatatgt attgataaat actttgtatg gaaatttatt
      421 taatatgatt gatgcagaaa aaatgtattt aaatgtaaat ccagatttta caaaagatta
      481 tgttttagga aatataagga tacatataag gccaagaata aaagctttat ttaatattat
      541 aataatgatt aataaaatta tgaataaaaa taaaggaaat aaggaaggtg atagtaatga
      601 gagcaacagg ttcgatacag agtcttatgg aaacaactct tgaaacaatt aaaggttcta
      661 tagatgcaaa tactattata ggagacccta taaaaacaga tactacagtg gttgtaccta
      721 tatcaaaggt tacaattggc tttggtatag gaggaggaga atactcaaaa ggatatgatg
      781 ataaagatag ggaaattgaa ttaaaaaatg aaaaatcaga tacgaatttt gctggaggta
      841 gcgcaggggc tatatctgta caaccagtag catttgtagt agttgaaagt ggagagacta
      901 gaataatgag tttagacagt aatatcaatc tagtggataa tatattatct ataactccta
      961 gagtattgga gaaaattcaa aatatatcac aaagtaataa gaatcaagat aaaaacaata
     1021 tgtaagaata aaatattttt aaatatgtat atagttttag gtaaagtagt ggggttagta
     1081 taaatcccac tattttgttg tgtactttaa acatttatat ctaataagat tttagtttaa
     1141 aacaatttaa atagataata aagttgatat atagtataat tataaaaaga aaagaagtat
     1201 tatattatag atatataaac gcatttgaga tataagaggg agttataaat cattagtttt
     1261 gaggtgaatg ttatgagcga taaagaatta tctattttgt tgttagaaag tatgaataat
     1321 tttcataatt taataaagat tattaataac gaaagatata aaaaagataa ggttcttaca
     1381 gaaagacagt tctttgcctt agtaaaaata agaaaacatg ataaaataga gcttaaaaat
     1441 ctaagtagag atttgcatgt atctacttct agtctgtgta tactacttaa taaattggtt
     1501 gagcaagagt atgtatatag ggaagaagat agtagagata gacgaaatac tttttatgga
     1561 ataactaaaa atggagaaaa gatattggat aatgaaatac taaagtttgt atctataata
     1621 agtgataaaa tggattgcct agatatagat aataaagata aattatttac ttctttggaa
     1681 gagtctaaaa atataataga acaattattt taaaaaggag aatttattat gaatataaag
     1741 tattctaaag aaatattaaa aagtttagaa aatgctataa cagtagtagc tctatttgag
     1801 ggagaagatg tttctacgac atttgataaa ataaatatga taagcagaaa ttttaattct
     1861 aaagaaaata gtatagatac tgtaacattg attgaaaata acaatttggt aaatgtttgt
     1921 cttgttggtt ttggtaagag agagttattg aatagagaaa aaattagaat cataggtggt
     1981 aatttgtgta aaaaattaaa aaatatattg aaaaatgaag catataagag tagtgatgtt
     2041 aatgtactga atcttaattt aaatagtgat gaaataggtt cttttgtaga agggatatta
     2101 cttggaaatt ataagtttga taagtataag acaaaaatta aggattttga aagaaaagaa
     2161 gttgatacta taactatatt tactgaaaaa gaagttgagt gtcaaataga aaaggctaaa
     2221 atattagcta attcaactat aatagctaga aatctagtta atgaaccatc aaacgtaata
     2281 tatccaaaaa cattagcagt agaaacagtt aaacttggtg cagagtttgg gtttaatgtt
     2341 gatgtttatg aagaagaaaa aataaaggca ttgggaatgg atgctttttt cgctgtttca
     2401 agaggctcaa ttaataaacc aagattcata gtaatgagat actttggaga taaagaaagt
     2461 acagatatat taggtttagt aggtaaagga ttgacttatg atactggtgg atattcttta
     2521 aaatcaaatg cttctatgct agatatgaag acagatatgg ctggtgctgc gagtgttata
     2581 ggtgctatgt gtgctatatc tcaaagtaaa ttaaagaaaa atgttatagc agtagtggct
     2641 gcatgtgaga atgctttatc aggaggttca tacaaacctg gagatataat tagttctatg
     2701 gcaaagaaaa ctattgaagt tttaaatacg gatgcagaag gtagacttac attagctgat
     2761 gcaatatact acattataaa caatgaaaaa gtaacaaagg tggttgatgt agctacatta
     2821 actggtgctg ctttaacatt gttaggtaat gttgcaactc ctatagtaac aaataatgat
     2881 gatttttatt gtgaattaga aaaagctgcc actttatcag gggaaagggt atggaagatg
     2941 ccaatttatg atgaatttaa agatatgata aaaggagaag aagcagacct taaaaacact
     3001 ggtggtaaaa atgctggatg tataacagca ggagctttta ttggtgaatt tgttgggaat
     3061 actccttgga tacatatgga tatagcgggg acaagtacat catcaaaatc aattggatac
     3121 aaagcaaagg gggctacagg agaacctgtt agaacattgt actatttggc agaaatctaa
     3181 aaaacaatta taaaatataa tttataaagc cacgtggata tttttgtaat ttaagattat
     3241 ctatgtggct ttatttttta tttaaataaa ttatttactc tttttctttt tttcaagtat
     3301 aaaagtatct ctattaaata aaagagcagg tatgtacata atactagtaa caaaaataaa
     3361 cgtaaaaata gcagttgtgc tatgacttaa aaaaccataa tagtacctag agttggtgaa
     3421 tgtgtttagt aaaaatatga atgaaaaata aataaaacat atagctaagt tatctctaag
     3481 tgcatatctg ttaactctaa attttctaac atcaaccata tatatatctg cataaattat
     3541 caagcactta gagaagataa agataaaata tattaagcta ggaaattcat agcccaagtt
     3601 tttagaaaaa atgagttctc caactgaaca aactaaactc caagaaaaaa atatattaaa
     3661 aagatgatac tgcttaaaaa ttagtatggc tatacacaga tatatacaaa tataattgat
     3721 tccaattggt aaagagttta gagtactata attacctgaa gaaataagta ataattgctc
     3781 tagagccata attattacaa gtaatgtaca aattatcttc tcaactgtat agctataagg
     3841 taaaatattt ttggttagtt ttggacaata gtataaaaat aatccaaata taaagagact
     3901 tacaaattgt tcactagaaa aaagaaaaat tttaaacata taacattctc tcctataata
     3961 aatttaatat gtatctatat aaatatattc attatatctc aatttaatat ataaaaaaga
     4021 taataatagt tagatttttt atatgaattt taataggtaa aatatcctat tgttttgtag
     4081 atatatccta attatgtcaa aaaataaaaa aatttttatt tattagagtt tttggaaata
     4141 ataataacag atattataat taattaacag tttttgtaag tatttgacaa tagagagggt
     4201 gtaacaaatg ggaataatag agtcagcatc aaaattggct gagatggttc atttattggc
     4261 tgttgaaaag gggataactg atatagaagc atgggatgaa gctgtgaaag aatacagcaa
     4321 aatttatgaa gaaagaagaa atgaatagaa tgataaaaaa tgaaaacttg atgataaaaa
     4381 ataactttat ttaaagaaat tcaattttta tagaagtatt aaaaataata taaataaaaa
     4441 gatggtattt caaatgtaag taaaaaatca ttttgggata ccatccttat tatttatttt
     4501 aataatatat aagtaataaa tcttacttat ccatattttt tatgtaacct cttttttcta
     4561 acttttttat atgctcctta cgtatttcat caagattaat accatattgt tcttctagct
     4621 taaacatcat agtaacacat gtctgcgcta catccattag ttccttagca gttttcttta
     4681 taatatcaac ttcatctaaa tcattatttt ctccacttag atttctgaat ttacctatac
     4741 attcagctaa ttcaccttgt tcttcaccca ttttgataaa tgtagattct agtgttgggt
     4801 ctaaattatt aagctctggt aaagatatag tttttagttt cataaaagct cctttcaagt
     4861 tattcgtatt tatactaaaa catcataaca aaaataatac taaaaaaaaa ctttaaattc
     4921 aacataaaaa cttacatata taataaaaat catgaaataa atctatattt gtcttaatac
     4981 agcctcatat aaaacttctc caataggttt atttattaca agagaagctg acttatcata
     5041 gactgtattt gctttattaa taagagcaat ataatcacct ttgtaataat ctataaaact
     5101 tgctgctggg tacacagcta atgatgtacc tcctataatc aataaatcag cattacttat
     5161 agcagaaatt gttttagtaa tgacatcact gtcaagagct tcctcataaa gtactacatc
     5221 tggttttact atagaaccac agttgtcaca gtaaggaata ttacctccca gatttaacat
     5281 agattctaag tcaaaaaact taccacattt agtacaataa tttctatgta cagaaccatg
     5341 aagttctagc acatttttac ttccagccat ttgatgaagt ccatcaatat tttgagttat
     5401 gacagcttta agcttaccca tttcttcgag ttttgctaaa gctatatgag cattattggg
     5461 ttttgcatta ggatatatta atttatcctt ataaaattca aaaaattctt ctggatatct
     5521 aacaaaaaaa gtatgtgaaa ctaattgttc agcagtaaat tgtttattga gtttttggct
     5581 aaataatcca gtagaacttc taaagtctgg aatattagat tcagttgaaa cacctgctcc
     5641 accaaaaaat acaatattat tgtgattagc tattaagtct tttaagctat ttgcatccat
     5701 agttacacct ccaaattatt atatataaat tgtactacat attaggtcgt ttatgtttta
     5761 ttaatttaaa taaatatata gtttaataag ttaaataaag ggaatataaa taaaatgtat
     5821 acagagtata aatagacaat atttactaaa ataacaagtt gtcgtataat tatattaaaa
     5881 ttgttttagt gtataagaag ggcgggggaa gtacgtgaag aaaaaagcag ctttagcaac
     5941 attggctatg ttacccttag gtgtggtaaa tgcacatgct gatggagata taggtatagt
     6001 gactataaat tatttaaatg taagaaatga accaactgcc gaaagtagca tagcctttgt
     6061 tgctaaaaaa gatgataaag ttttgattaa agactcttct aatggatggt ataaaataaa
     6121 agctgaatct ggacaagaag gttgggcttc atcaaaatat atagcaaaat caaatagtga
     6181 ctctttaaga acgtctacaa ataaagaaaa acaagttatt tcaaatagtt taaatatgag
     6241 aaatggagca ggtacaagtt acagagtaat aactgtacta aaaaaaggtc aaaaagtaga
     6301 agtaatatca gagagtaatg gatggtctaa aatcaaatat gatggaagac taggatatgt
     6361 atctagctct tatttaggag acgtttcaaa ctcaactaat aaatcaaaaa ctaaacaagt
     6421 gaatacaact tcactaaatg taaggagtgg accaaataca agttatggtt tgttaggcaa
     6481 gttgccaaaa ggaagtaaag tagaagtaat atcagaaagt aatggatggt ccaaaataaa
     6541 gtataatgga aaagatgcct atgtatctag tatgtactta tcggatgtaa gtcaaagtaa
     6601 ttcagacaat tctagtcaaa gcaatgataa aaagaatact gataaggttg taaatacagc
     6661 ttctttaaat gtaagaagtg gaccaggttc tacatatagt aagttgggaa aagtttataa
     6721 aggaagtaaa gtaactgtac tatcagaaag tagtggatgg gctaagatta attttaacaa
     6781 taaagaagca tttgtagtag gcaattattt atctacctca gcagatactt caaataataa
     6841 ctcaaatagt aactctgata acagttctaa tagtaatggt aataattctt catcatcagg
     6901 ccaagtaaat ggtatgtcag gtataagtgg ggctaaaatt gattataaat ctttgagtta
     6961 tactttagaa tctcatataa gtaaacaagt tgagaaagca gcatcaggag gaaatgtgat
     7021 agctccaagt aataggaaaa gtactccaag tcctgagttt agtacttttt cagctcaaag
     7081 aacaagctca tttgtaaatg caagttcaag tgatatagaa tattatttaa atcctaagaa
     7141 ttttacaaat actactaaag gtatgatgca gtttttaaag attaacagtt atagagatgg
     7201 tatttcagaa tcaagtctta actcgtatct taatggttta tcttctagtg tatttaaaaa
     7261 ccagggagca gcctttataa atgctgccaa gaagtataat atagatgttg tatatctagt
     7321 atctcatgct atgtgggaaa ctgcttatgg taagtctaca cttgcccaag gtcaaacatt
     7381 aacatcttat aagggacaac ctcttagtaa gccagttaaa gtatacaatt tttttggtat
     7441 aggagcaata gataaaagtg ctaatgtttc aggtgcagaa gcagcgtatt ccaatggctg
     7501 gactagtgta gaagctacaa tagatggctc tgcaaagtgg atatcacaaa attacgtaaa
     7561 tagttctaag tacaatcaga atactatcta caagatgaag tggaattatg attatacatg
     7621 gcatcagtat gcaactgatg taaactgggc taatggaatt tctggaatta tggaaaattt
     7681 aattggtctt tatggaggag gaagtagttt ggtttttgaa gttcctcaat ataagtaata
     7741 cataaataaa aaatatagac tatgggtagt tttcatttta tttaaattat ctatagtctt
     7801 tttgtatata atttataatc tgtaaattta atgttaacaa aacttttatg ttataatatt
     7861 aattctaagt aaatataagt gagtaatagg tagaaataga taaaaagtgg taataatata
     7921 tataaagata attttgagaa aggaggatat atatggaaag tattaaggaa tatttggata
     7981 agttagaaat taataatagt ggtcttggaa agcaacttaa agaagtttat ataaatagag
     8041 tagtttattt taaggaagat aagatagttt atttttatct aacttctaaa gatattgttt
     8101 ctcatgaact tttagataaa tttaaagaag aattgatgta taaactcgat tattttaaag
     8161 atatgaggat gaagataaga tttactggtc ttgaaagaaa atccaataaa gatgttataa
     8221 aaaaatattg gaataatata ttgtatatat taaagtatct ttgtccatct attgcagggt
     8281 ggtataaaca agtagaattt ctttgtttag aagaagaatt aaaaataaaa cttccaaaag
     8341 gtatatttta tgagcgatta atgaaaaaaa atgtaactta tgtacttaaa accgtgctta
     8401 gcgaagagtt gggattagat ttaaatataa caatagaaaa agctgttgat gaaaaggtaa
     8461 ataaagaaag actcataaga atcaatgata gagaaatgga agaaaaaatt agggctttag
     8521 aaattggaaa agttaataac tgtgaagaaa atgaagagga aagctatgtc attaagtctg
     8581 atgtagatga aaatttaatc tatggagata atgcaaatgc tatgattgaa aatatagttg
     8641 aattaaatgc atcttctgga acagttgcag ttgttggaga tatatttgat gttgacacta
     8701 aagaacttaa aaacggaaaa atacttatga tagcttcaat tacagattat actagctcaa
     8761 taagctgtaa acttttttta acagatacaa ataaggatgg agtacttgaa aatgttaaaa
     8821 aaggagcata cttaaaaatc aagggtgaca tagtttatga tacatatcaa agagaaatta
     8881 gtatgatgat tagtgggata agaaaagaaa ctagaataga gagagaagat aaatcagagg
     8941 aaaaaagagt agaactccat gcacatacac aaatgtcttc tatggatgct atttgttctg
     9001 ttaaaaagct tgtagagcgt gcagccaaat ggggtcattc tgctattgct ataacagacc
     9061 atggtgttgt acaaggattt ccagatgcta tgaatgcagg taagtcaaat aatataaaaa
     9121 tactttatgg agtagaaggg tacctggttg aagatgattc tctcattata gaggatgcaa
     9181 atgataaaga actttcgcag acatttgttg tatttgacat agagacaact ggcttttcta
     9241 atacaaatga taaaataact gagattggtg ctgtaaaaat agaaaacttt aagattgtag
     9301 atagatttag tgagttaata aatcctgaaa aagatatttc ttataagata caggaattga
     9361 caggaataac taatgaatta attaaagaca agccaaccat tgaagaagtt cttcctaagt
     9421 ttatggaatt tgttggagat agtgtattag tagcacataa tgcagagttt gatactggat
     9481 ttatatcgca aaaatgtagg gaacaaggct tgttatataa taacaaaaaa gtagatacac
     9541 ttatgttagc aagagtaatg ttgccaaact taaaaagaca tagattaaat gtcgttgcaa
     9601 aagaacttgg tattcctctt ttaaatcacc atagagcagt ggatgatgct gaagctacag
     9661 cacttatatt taataagttt ttgcagatgc ttacagaaaa aggtgcaaaa actctaagtg
     9721 atgtaaacaa tatacttgga aagattgatt acacaaagct tagcacaaat catataacct
     9781 tgatagcaaa aaattctata ggtataaaaa atttatataa aataatttca gatgcacatg
     9841 taaatcattt ttttagagca ccaagaatcc ttagaagtgt attaaatgaa tataaagaag
     9901 gattaataat tggttcggct tgtgaagctg gggtggtttt tcaagctgta aagaaaaacg
     9961 tgtctgatga ggaaatgaaa aaaataatag atttatacga ttatattgaa gtaatgccta
    10021 tagataataa tagatttatg attgataaag gagaagtaaa ggacgaagaa gagttaaggg
    10081 agttgaatag gaaactaatt gatacagcca aaaaatttga caaaattcca gtagctactg
    10141 gggatgttca ttttttagac aaacatgaag ctgtcttaag aaaagttctt aaatattctc
    10201 aaggttttaa agtagatgaa gaagaaactt atttacattt tagaacaaca gatgaaatgt
    10261 tggaagagtt tagttatctt ggagaagaat tggcatatga agttgtagtt gaaaatagta
    10321 atctgattgc cgatatggtt gaagatataa aacctatacc agatgatact tatcctccaa
    10381 ttatagaggg ctcagatgtg gagttaagag aaatgtgtta caaaaaagcc aaacgaatct
    10441 atggagaccc aataccagaa atagttcaaa aaagactgga tagagaatta aattcaataa
    10501 taagcaatgg gtatgcagtt atgtatatta tagctcaaaa actggttgca aaatctcttt
    10561 cagatggata tttagttggt tcaagaggct cagttggctc atcttttgca gctactatga
    10621 gtgatataac agaagttaat cctcttccag cacattatat ttgtgaaaac gaagactgta
    10681 aatattcatt cttttatgaa ataggtgaat ggggctcagg tgttgattta ccagacaagg
    10741 attgtccaaa gtgtggaaga aaacttgcta aaaacggtca tgatattcca tttgaggttt
    10801 tcttaggttt tgaaggtgat aaggaaccag atatagactt aaatttctca ggaaattatc
    10861 agccaacaat tcataaatac acagaagaac tatttggaga ggggtatgta tatagggctg
    10921 ggactatagg tactgtagca gaaaaaacag cttttggata cgcaagaaaa tatgtggaag
    10981 aaaataatat aagtgtacca aatgcagaag tacttaggct ttcaaatgga tgtactggag
    11041 taaaaagaac ctcaggacaa catcctggtg gagttatggt tataccagac tataaagatg
    11101 tatacgattt tacacctata cagtatccag caaatgatac ttcttgtggt gttataacta
    11161 ctcactttga ttatcattca ataagtggta gaatacttaa acttgatata cttggacacg
    11221 acggtcctac cattataagg atgcttgaag atatgactgg aataaatata acagaaattc
    11281 ctttagatga taaagagact atgtctctat ttacatctac agaagcatta ggtgttacac
    11341 cagaagaaat aaactgtcct ataggttgcc ttgctgtacc agagtttgga actaagtttg
    11401 tacgacaaat gttacttgac acaaaaccta ctacatttgc agaacttgta cgtatatctg
    11461 gtctctctca tgggacagat gtttgggtaa ataatgcaca agacttagtt agagaagata
    11521 tagttggatt aaaagatgtt atatctacac gagatgacat aatgaactat ttaatattta
    11581 aaggattacc tcccaaaatg agctttacaa taatggagag tgtaagaaaa ggtaaaggtt
    11641 taaaaccaga acacattgaa gaaatgaaaa aaaataatgt tccagaatgg tatataggct
    11701 catgtaaaaa gattaagtac atgttcccaa aagcacatgc tgttgcttat gtaatgacat
    11761 catttagaat tgcatattgt aaagttcact atccagaagc attttatgcg acttatttta
    11821 cgacaaaagt tgaggatttt gatgcagatt taattgttaa aggattagat gctataaaat
    11881 caaaaataaa tgaaattgag tcactgggaa atgatgcaac cacaaaagaa aaaggtatgt
    11941 tgaccgtttt agaagttgct cttgaaatgt atgctagagg aataaaatta ttaccagttg
    12001 atatatataa atcagatgca actgagttta tagttgtagg agaaaaaaca ttacttccac
    12061 caatggcagc aatacaaggt cttggaggaa atgcggctat aaacatccaa aatgaaagaa
    12121 aaaatggtga atttatatca aaagaagatt tgagaaagag aacaaaaata tcaaaaacgg
    12181 taatagaaac tcttactaat catggttcac ttgagaatat gagtgagaga aatcaattgt
    12241 cattgtttta agttagccca aaaagttgca aataaaagaa ttttatgata tcatagtata
    12301 gaaaatagta tactttgcag aaactaagag tgagacagct gttctcactc ttttatttta
    12361 aatatattat taaaaagctg gtttattttt attaaatata ttttatttaa gattatataa
    12421 actggtcatt tacaattgaa taggaggaac tttaatgaaa aaaaatttag aagctacaat
    12481 tgaggagatt gtaactaaaa taacggatga acatggattt gaaatggttg atgttgaata
    12541 tgtaaaagaa gcaggagaat actacttaag agtatacata gataaagaag aaggaatatc
    12601 tttaaatgaa tgtgaattag ttagtagaga attaagtcca atactagatg aaaaagaccc
    12661 tataaaagaa aattactttt tagaggtatc ttctccaggg cttgatagag cattaaaaaa
    12721 agacagagat tttgttagat atcaaggtag agatgtagat ttaaaattat ataaaccatt
    12781 aaatggatgt aagcaatttg aaggtgaact tgtaggatta acagaagata ataatataaa
    12841 aatcattgca aatggtaagg aaatggaatt taatagaaaa gatgttgcta ttgtcagatt
    12901 agcaataaag ttttagtggt atattaaata gggaggatat aaaaaatgaa tcatgaattt
    12961 atggaagcat tagatgaatt agtagttgac agaggtatag ataaagaaat acttatagat
    13021 actatagaac aagctctttt aactgcatac aagaaaaact ttggttcagc acaaaatgtt
    13081 agagttgaat ttgatagaga caagggagat ataaaagtat tttctcaaag agttgttgtt
    13141 gatgaatcag atttatatga tactttctta gaaatagagt tagcagatgc tagagagata
    13201 aatcctaatt atgaattagg tgatattata gaacatgaag ttacacctaa aaactttgga
    13261 agaattgcag ctcaaacagc aaagcaagta gttgttcaaa gaataaggga agctgagaga
    13321 gaaatagtgt ataatgaatt tatagaaaaa gaaaatgaaa tagttacagg agaggttgct
    13381 agagcaaata aaaatgttgt ttatgtaaat cttggaagaa tagaaggtat aatgactcaa
    13441 agtgaacaaa taccatttga gaattataaa gcaggtcaaa aaataaaatt ctatatactt
    13501 gaagttaaaa agactaacaa aggacctcaa atagttgtat caagaagtca tccgggtcta
    13561 gtaaagagat tgtttgaaga agaagttcct gaaatatatg aaggaatagt tcaaataaaa
    13621 tctgtagcaa gagaagcagg gtcaagaact aaaatggcag taagatctat agatgaaaaa
    13681 atagacccaa ttggagcttg tgttggacca aaaggactta gagtaaaaaa tatagtggat
    13741 gaacttggcg atgagaaaat agatataata aaatatagtg aagacccagc agagtttata
    13801 tcagcagcac ttagcccatc aaaggttgtt agagtagata tagatgaaga tgaaaagtct
    13861 gccttagtaa tcgttccaga ttatcagtta tcgcttgcta taggtaaaga aggtcaaaat
    13921 gcaagactag cagcaaaatt gactaactgg aaaatagata ttaagagcga atctcaagca
    13981 gaacaagaaa tgttaaatct tgaaaattcc aaagaaatag aagctgagga tgttgaagct
    14041 gaatttatcg accaagaaga gttagatact tcagaggata attttagtga agaagagtaa
    14101 ggagggatga ttttgaaaaa agtaaaaaaa gtgcctcaaa gaaaatgcat agcatgtcaa
    14161 gatagagact ctaaaaaaga gcttataaga atagttaaaa ataaagaagg aaaaatattt
    14221 ttagacccaa caggaagagc caatggaaga ggagcatatg tatgtaaaag ctctgaatgt
    14281 ctaaaaaaag ctataaagag taaagcatta aataaagctt ttaaaataga tgttcctgat
    14341 gaagtctatg ataatctact tatggagtta gaagaatatg aaaaataata aagaaaaagt
    14401 actttcgttc ttagggctcg caactcgtgc agggaaaata gtgtctggtg atgattcaac
    14461 tttgcttgac cttaaaaaag gaaaagtaaa tttgatttta atcgcagaag atgcatcaaa
    14521 taatactaaa aaacttttta aagataaatc aacttttaga aatattccat atctattttt
    14581 ttcaacaaaa gaggaaatag gatttgcaat aggaaaatct ccaagagcag ttgtaggaat
    14641 aaaagatgaa aatttttcta aaaagataat tgaactaata gaaatttaaa ataaaatagg
    14701 gggtgatctt gtgtcaaaaa caagagtata ccaaatagca gaagaattaa atatatcaaa
    14761 tgaagagctt ataaataagt tagctgaatt agatataaat gtaactgaca aagattcagt
    14821 attagaaggt gaagagttag aattagcact tgaaatgtta ggtgaagatt tatctcaaga
    14881 aaatggaaat gttatagaaa tagatggaaa gttgacagta caagtattag caacgaaatt
    14941 agataaatct ccatcagaaa taattatgaa gcttatgaaa atgggaacta tggcaacaat
    15001 aaatcaagag ataagttttg agattgctgc tttggctgcc aaagactatg gatttgaatt
    15061 aacagtagct gaaagtgatg atacagaagc tttagaaata gaagctttaa tggaaattga
    15121 agaagacaaa gaagaggatt taaaacctag accaccagtt gttacagtta tgggacatgt
    15181 tgaccatggt aagacatctt tactagatgc aataagaaaa actgatgtga tctcaggtga
    15241 agctggagga ataactcagc atataggtgc ttcagaagtt aaaatcaatg gacacaaaat
    15301 agtattttta gatactccag gacatgaggc tttcacatca atgagagcaa gaggagcaca
    15361 agttacagat atagcaatat tagtagttgc agcagatgat ggtataatgc ctcaaacagt
    15421 tgaagctata aaccatgcta aagcagcagg tgtaccttta atagttgcta ttaataaaat
    15481 agataaacca ggtgcaaacc cagataaggt taaacaggaa ttagcagacc aaggattatt
    15541 agttgaagac tggggtggag aagtaatagc agttccagta tcagctaaga aaaaagaagg
    15601 aatagataca cttttagaaa tggtattatt ggttgctgaa atggaagagt taagagctaa
    15661 tccaaataaa agagcagtag gaactgttat agaagctgag cttgataaag gaagagggcc
    15721 agttgcaaca gtacttgttc aaggtggtac tttaacagta ggtgacccaa tcgttgcagg
    15781 tgttgcatgt ggtaaagtta gagcaatgat aaatgcaaaa ggtaagagag taaaaacagc
    15841 aggaccatct acagcagtcg agatattagg tctatcagaa gttccacaag gaggagacca
    15901 atttgtcgaa gttccaactg ataagattgc tagaagtgta gcagcaagaa gacaacaaat
    15961 agttagagat gaaatgttaa aatcaactca aagattatca ctagatgctc tatttagtca
    16021 aatgagtgaa ggttctataa aagaccttaa tatagttata aaagcagatg tacaaggttc
    16081 agtacaggca gttaaacaat ctttagaaaa attatcaaat gaagaagttc aagttaaagt
    16141 aatacatggt ggtgttggag ctgtaacaga atcagacata ttacttgcag cagcttctaa
    16201 cgcaataata ataggattca atgtaagacc agttccaggt gcagaatctc taggagaaaa
    16261 agaaaatgtt gatataagaa catatactat aatatacaag gcaatagaag atatacaagc
    16321 ggctatgact ggtatgttag accctgaata tgttgatgaa gaaacaggaa aagctgaaat
    16381 aagagaaata tataaaatat ctggtgttgg aacagtagct ggatgttatg tgactaatgg
    16441 taagatattt agaaattgta aagtaagatt agttagagat agcataataa tacatgaagg
    16501 tgagcttgca gcacttaaga gatttaaaga tgatgtaaaa gaagttaatt ctggatacga
    16561 atgtggtatg agttttgtaa attataacga cataaaagaa ggcgatatag tagaagctta
    16621 tataactaaa gaagtagaaa gaaaattata aattcgtatc aaagaaaatc agttagtaaa
    16681 gaaggtcgat ggtatggcat catataatag aacaagaagg atagcagaag aaataagaaa
    16741 agtcgtaagt actatgctta taaatggagt aaaagaccct agaataacat ctatggtgag
    16801 tgtaactgat gtagaagtta caaatgacct aagatatgct tatgtatatg taagtattct
    16861 aggcggagat gaagaatcca cactaacagg tcttaaaagt gctggaggat atataagaag
    16921 agaagtagga aaaaatataa aacttagata tattcctgag atagtattta aacttgatga
    16981 ttcaatagaa aaaggaatgt atatggatag ccttataaaa agagttaatg aaaaaaatgc
    17041 tcaacaaaat gaggatgaaa attatgatga ataatatgga tagtattata aactatattt
    17101 atgagggtga ctattttata gttacctctc atatcagtcc agatggagac aatattggtt
    17161 ctacactttc aatgtattat acattgaaga atttaggaaa aaatgtatat tatgtacttg
    17221 atgatgaagc tccattaaat ttaaggtttt tggttgaagg tgtaaaaatc tataagtcta
    17281 atgaatttaa tatgaagaac tattctctaa ttgcacttga ttgtggggat aagatgagaa
    17341 tatgtgtgag tgatgaaatc aaaaataatg catcaaaatt aatctgtatt gaccatcatg
    17401 ctagtaatga ttcatatgga gatttaaatt atgtagatat tgatgcatct tctacatgtg
    17461 aattagttta taatttatta gttagatttc agcaaataaa agatattaat attataaatg
    17521 aagatatagc aacatgttta tatacaggtc ttgtaactga tactggaaac ttttcgtatt
    17581 caaatgtaca tgcaagtagc tttgaaatgg caaaaaattt acttgtttta ggtgcacaaa
    17641 aaaatactat aatacaaaat atatatcaaa gcaattcttc tggttattac aaattattag
    17701 gagaagctct aaagggtttg gagatatttg attaaaaaat agttacgcac actcgtgact
    17761 ttagtcatga gttagtagct aaaatagtca gcatataggg aaacttatat gtagagatag
    17821 gata
//
LOCUS       DAMJWS010000088        17474 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMJWS010000088 DAMJWS010000000
VERSION     DAMJWS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.089"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            163..909
                     /locus_tag="PC399_003039"
     CDS             163..909
                     /locus_tag="PC399_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF2593884.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            970..1644
                     /locus_tag="PC399_003040"
     CDS             970..1644
                     /locus_tag="PC399_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2593885.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            2013..3359
                     /locus_tag="PC399_003041"
     CDS             2013..3359
                     /locus_tag="PC399_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2593886.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3386..4786
                     /locus_tag="PC399_003042"
     CDS             3386..4786
                     /locus_tag="PC399_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593887.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYKNLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4996..6432
                     /locus_tag="PC399_003043"
     CDS             4996..6432
                     /locus_tag="PC399_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2593888.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            6776..7720
                     /locus_tag="PC399_003044"
     CDS             6776..7720
                     /locus_tag="PC399_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF2593889.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7889..8821
                     /locus_tag="PC399_003045"
     CDS             7889..8821
                     /locus_tag="PC399_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF2593890.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENTLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8926..9393
                     /locus_tag="PC399_003046"
     CDS             8926..9393
                     /locus_tag="PC399_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF2593891.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9637..10491)
                     /locus_tag="PC399_003047"
     CDS             complement(9637..10491)
                     /locus_tag="PC399_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF2593892.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10657..12621
                     /locus_tag="PC399_003048"
     CDS             10657..12621
                     /locus_tag="PC399_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2593893.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12621..13082
                     /locus_tag="PC399_003049"
     CDS             12621..13082
                     /locus_tag="PC399_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2593894.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            13085..13405
                     /locus_tag="PC399_003050"
     CDS             13085..13405
                     /locus_tag="PC399_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2593895.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13421..14521
                     /locus_tag="PC399_003051"
     CDS             13421..14521
                     /locus_tag="PC399_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2593896.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14641..17241
                     /locus_tag="PC399_003052"
     CDS             14641..17241
                     /locus_tag="PC399_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF2593897.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDENIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYHIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6959 a         1783 c         3006 g         5726 t
ORIGIN      
        1 aaaaatctaa atagttttgt ttaaatatat ttttatattt tgtaatttca aattaataaa
       61 taattagatt taataaacaa aaaataaaaa tgttgattat attaataaac tttaatataa
      121 tatatttatg aaaaatgtaa actgtgaaat ggaggtagta acatgaaaat tgaaaatctt
      181 tctattgatg atattaaacg aggctatata tttaatgatg atttaaattg ttatagttgc
      241 attaaatgta caaaaacata tgaggtagga gaaatatata aaataggtaa tagattcttt
      301 gaagcttcaa aagctattga acttcatatg gaattagaac atagtgatta tttagagcaa
      361 ttgcttaata gtgattcaaa atacaacaca ttgactgaaa atcaaaaaca attatttcaa
      421 ttatttatta gtgatttacc agataaggag atagcaaaac aggttggagt atccacatct
      481 actatacgcc atcaaaaatt tattttcaga gaaaaagcaa aacaggctaa gctctattta
      541 gcactatatg aatctgtatt tgaagataaa tctaataaaa gtagtactat aataccaatc
      601 catgaacatg ctcgaatggt agatgaaaga tatgtagtaa ctgaagaaga gagaaaacac
      661 attttggata catcatttga aagtatgaat cctttaaaat taaaagtatt ttcttcaaaa
      721 gaaaagaaaa aggtcgtaat tttatctaag attttggaac aatttgaacg tgggaagaaa
      781 tattcagaaa aggaagttaa tcagatttta gaatctatat atgatgattt tgcaacaatt
      841 agaagatatc taattgagta tggttttatg agtagaaata aagaatgtac aaaatattgg
      901 ttaacataat taaaataata aattttacta atgtgtaatg ttatttttat ctagggggag
      961 gagtcaaata tgaatgatga attaaaatct attttataca ataaaggtgt agacattgtt
     1021 cgctttgttg atatatctga atttccaata aatcaaacac aaggtttttc taaagcaatt
     1081 ttattttgta tagggttatc aaaaaaattt attacagata tatacaataa cttacctaca
     1141 gatagtgatg agtttttgga aaaagaggag aaagtagaag aattagctga ctggatttca
     1201 aaatatattc aaaataaagg atatagagca tattctcaat cggagaaaaa caatctagaa
     1261 catggatatt ttgaaaaagc ctatattaat ccagaaatgc aatcagggat tagtccttta
     1321 ccacataaga caatagcaaa tattagtggc attggattta tgggaaaaaa taatttgttt
     1381 gtaacagagg aatatggatg tgcttttagc atgtgtacag tacttacaga tgctccaatt
     1441 tctgttgaaa gatatccttt gattgattca aaatgtatgg attgcaatgt ttgtgtagag
     1501 aactgtcctg caaaagctat acatggaaat gaatggactt tacctggaaa aagagaatct
     1561 attattgatg tttcaaagtg tttttgtgta ttgaggtgta tgatgagttg cccatggtca
     1621 ttaagatatg caaatcaaaa ataactttac tagagaaggt attaaattga atattataaa
     1681 atttttaaaa tgtaagatta tataagattt ctcaaaataa aaatttgtat agaacactca
     1741 gaaatgagtg tttttttatt gggattaaat tatattgata caaagcttaa ctattttaat
     1801 cattatataa aatttgtatt aaaaaattaa gaataacttg gtaatagtga aaatataatg
     1861 agattataat tgatttataa tataactata atgatattgt aaaaatgaaa attgtcctct
     1921 caaaattccc aaagagagta aaagtcactt cagttatagt caaaaaaatt tttctgtgaa
     1981 aagaatttag atttgaaaag gaggaattaa tcatggaaat tgaaactaaa actccagatt
     2041 ggattaatgt actagaatta aacagtaaaa tagatattac tgggaaatat gatgtaagta
     2101 atatgataca aaatattgtc tctgataagt cactagaagg ttccattata tattttccaa
     2161 aaggtaatta cttatttgag aaaggtatta aaattagtca acaaataaca cttcaaggag
     2221 attcttatta tggaggaggt aatcaagtat caaatttaga taaaaaacaa tcaatcatag
     2281 gaacaaccaa ttttataacc cgaggagttt ctaatatgag tattattaca ttgtcaggaa
     2341 caagtcaatg tatcaaaaat attaattttt attatgatag tcatgatata gagaaaatac
     2401 cgcctaaaaa tgtatctgct attacagaat atggagaaac acaaggacta tctcattttg
     2461 aacatctgtt tatttctgga ttttcaggga taggaattga aattccatac tattcaactg
     2521 gtaatgatat tactgttagt tcatgtggtc ttggtatgag gcttggagag aagtcaatgc
     2581 tttctagtag caagatttac gaatgtaaaa atggaatgga gattactact ggtgttagct
     2641 taaataatgt gagaatagaa gaaataaaag aaataggtat taataataaa ggatttggtt
     2701 ttaacctgat tatgaatctt atggtcgacc aatgtggata ttgtggattt atgtttgaaa
     2761 aaatgtgcta tagccagata actgccagaa ttactagatg cggtcagtat tataaaagtg
     2821 ttgattataa tgcttatgag aatatggaag ataggaaaga agaagcttat tccatttttt
     2881 atggagatat tttagaaaat tgcaatattg tattgatgaa tagtaatatt gataccttgg
     2941 aaaatgattc acttgatgaa cataagatat atgtaatcaa ggcaaatgaa acaaacaatg
     3001 taatgctaac atgttatgct gaagcagata actttataca atgtgcaaag ggcaacttgc
     3061 tgttagaaaa tggtgaaaat acttataaat tttataatgg tgagatttgt tctgtaggag
     3121 gtgttcaaat ttctgatata gatgaaaaag atttaatcaa aataatagag aacactttgt
     3181 atatagatga taaaggtaag tatttaatgg ttcctagatt aaaaaagaat actgtaatat
     3241 attcagtact agattctaca gaagaaataa ataaattaca tggtggaata tgggaacaga
     3301 ttgatgtaaa agtattagct ggggaatcca tgtactatta taaaaagata aaagattaaa
     3361 ttatcaaact aggaggtagt gattaatgga agagtctata ataattaaag atataagtgg
     3421 ctggattaat gtccttgatt atggaatttg taatgatgaa acaaaagatg taagttttaa
     3481 gttgcaagag attatggatg ctgtaccaga agattctatt atatattttc caaaaggaaa
     3541 atacttattt gaaagtggtg ttaagattaa gaaacgatta actttatgtg gagattctta
     3601 tattttggcg aatgttccta aaaatactgc tggagtaaca caatttactt ttaaaggaaa
     3661 gaaaaatcct aaaagtgaaa atatagcaat tataacagca aaaagtgtaa gacactgtat
     3721 taagagcatt tgcttttatt cagatagttg tgatagagat atgacatcta atcatgaacc
     3781 tccaacagat ggaaaaccta gatttcatca taatgtaatt ataaattata gaaatatatc
     3841 ggctattgta tgtgaagata aacagggaga atcaggtcat tataaaaatc tatcttttag
     3901 tggtttttct ggaattgcac ttgatatgcc taataattct acagcaaatg atattacaat
     3961 ttttacatcc ggacttggaa ttagtacagg agaaaattca actattgcta acagtaaggt
     4021 ttggggctgt gttgatggaa tgataatttc tactggaact tttataaatg gtatgagggt
     4081 agaagaaata aaaaaagttg gaattaaaaa tattggtaga ggtttaaatt ttattacaaa
     4141 tatcacaatt gaccaatgtg gatattgtgg agtttggttt gattctatat ccaatgttca
     4201 gatatcagga aatatcacaa ggtgtgggca gtactattat gatgtaagct atgataatta
     4261 tttaatgttg gataatagag ttaaagaagc atattcttta ttctatggta atttattgga
     4321 atcaagtaat atagttttat caaataccaa taaagataat tgggaagatt tcaaagaaga
     4381 taagcataag gtgtatcttg tagaagcact tgaaaccaag aatgtactat taacatgcaa
     4441 tatagatgct actgactgga ttgttaagtc agaaaaagga aatttaacgt acaataatat
     4501 gcgacaaact gttatatttt acgatggaaa actaagtagt atcaatggaa ttggtatatc
     4561 tgatgaagat tatggggata aagtcaagat aaaaaaagga aatatatata taaatagtaa
     4621 ggataaggat attaagatgc ataaaccaga ggtaggtgac attatgtcaa caattattaa
     4681 agataaaagt attctttctg ctcattatgg gggaacatgg gagaaaattg gtgaaaaaat
     4741 agagtttgga ataactgtac actattataa attgatagag aaataagtaa atacgtagta
     4801 tttgaataga tgtattttat tggagagcta ggactatagt tttaatgtaa atggtttggt
     4861 aatagcatgg attgaataat ataaaaataa tataaaaaat taatttgatt atgtaaaatt
     4921 ctatgaagta gtaaaactgt gactttagtc ataggaggtt caaactaagt aaaagggaaa
     4981 ggaatatata atattatgga aggaagaact gggattaaag atggaagatg gattaatgta
     5041 gtagaaagag gtattaaaaa tgatggttcg tacaacgtag gtcctgcttt gcaggatatt
     5101 attgatgaaa tagaaacaga tttttgtgtc ttatattttc caaaaggtaa atacctattt
     5161 acaagtggtg ttaaaattaa taaagaaatt gtattgcaag gtgattctaa ttcatctaca
     5221 ggcactacac aatttataac tcgtggagta gaaaatatga gtattattac attgacaggg
     5281 aaaaaacaat gtattaaaaa tataaatttt tattctgata gttgtgagat acaggtaaat
     5341 aaagaaccac cgacaaaagg taatcctaaa tatcattatg agatgattat taataagaat
     5401 gaaaagaatg ttgagatgaa taatgtatca gcaatcctgt atgataacag taagaatatg
     5461 attaaaggtc taggtcatta tgaaaatatt tatataagtg gtttttcggg tacaggaatt
     5521 aggataccat attactcaat tgtaaatgat attacagttt catcttgtgg gctgggtatt
     5581 gatacaggaa tagacacaat tatttctaat agtagaatat ctaagtgtca aaatggaatg
     5641 aggattagaa ctggaactag tataaataat gtaaggattg aaaaaataca gaaagttggt
     5701 cttataagta gtcaagttaa ggggtatgag gggtatggaa gttataaaat taataatatt
     5761 acgattgacc aatgtgggta ttgtggcttt tcatttgatt caatgaaaga tagttatttt
     5821 tctggtatta taagaaggtg tggtcagtat tattataata cagattatga cacatattta
     5881 agtattaaag atagagtaga agaagcatat tctttatttt atggaaatta ttttacagaa
     5941 tgtaatattg atttagttag tgataataag gataactggg atgatggctt agaatataaa
     6001 cataaagttt atgtatttaa aacccttcaa ataccaaaat taacattgag atgcaatata
     6061 gatgcctatg attatatcgt taaatcggta catggaggag gtaatttaat gttaaaaaat
     6121 aattttaaca attatatatt tattgattca tctaatccag aagttattaa tggaattaat
     6181 tttaattatc aaaagaatgg aaacttgtta aggttaaata atggtaaatt aagttataaa
     6241 aatggaaagg aagaaacagc tatattcaga cctaattatg gtcttattgt agcatcaaca
     6301 gattcagaat gtgaacaaat agagactagc tatggaggaa aatgggagaa gcttggttct
     6361 gaagtaattg gagaagaaac ggtatattat tataaacatc atgaaccaga taaaacaaac
     6421 caaatacaat aaaatttgta ttatataatg atataaagtt ttaatatata gtgtttacct
     6481 ttaatgtaat attggtatat ttgtatagta ttaaatttgt taaaaagtaa aattgtaggg
     6541 tgcttgaaaa tagcaccttt tttagtttca ttataaaatt agttttgaaa gtttaggaat
     6601 aatgtatatt tatttttaaa gaattataac cttattaagg atattttaaa tgctagaaat
     6661 gaatcagtaa atcatactgg aattatctta aatgttttga ttaaattaag tatttagagc
     6721 tataattata ttaaaaatat attagaacat aattatatta ctatggaggt taaatatgat
     6781 agtaagtgtg agtagaagaa ctgatatacc tgctttctat agtaagtggt tttttaacag
     6841 acttaaagaa ggttttgttt atgtaattaa tccaatgaat ccaaaacagg ttagtaagat
     6901 agagttaaat ccacatactg ttgattgttt tgtattttgg acaaaggatg taactccaat
     6961 gttatatgat ttaaataaat taaaagacta taaatattat tttcactata ctataacatc
     7021 atatggaaaa gaagtagaga catgtatttt agataaaaga aaggtaatag attcatttaa
     7081 ggaattgtct agaaaaatag gcaaagaaag agtaatttta agatacgacc cgattttctt
     7141 aagtgaaaag tacacagtag attatcatat caaagcattt gcgagtttat gtaatcagct
     7201 tgatggattt acagaaaaat gtataataag ttttatcgat ttatataaga aaacaaaatt
     7261 taatactaaa gcattacata taaagccaat agaaattaca gaaatagaaa cattatcaaa
     7321 agagatttct aagattgctc ataagcatgg gataacactt gaaatgtgtt ctgaagaata
     7381 taatttaagt aaatttggga ttggaaaaag taagtgtatt gatgatagat taatatcaaa
     7441 aattatagga tgtgaagtag aggtaaaaaa agatagtaca caaagagata tttgtggatg
     7501 tgttaaaagt gtcgatatag gacaatacaa tacttgcaga cattattgtc tgtattgtta
     7561 tgctaacttt aattatgcaa aagtagaaga aaactgtagg ctttataaag aagataataa
     7621 attactcata gatactttaa gagatgatgc aaaaataagc attagagata tgaagactat
     7681 taaagtagat aagaataaac aaattagtat atttgattaa aataaatata ctaattacct
     7741 taatatatta agaatataga tacttttgat aacaaaatta aacttaatag atattttcta
     7801 tataagaaag atggtcataa tatgtcaatt tgaccatctt tttgatataa ttaaaacaca
     7861 aataatttag taacaaagga ggcagaactt gaatttatta aaaaaatatc ctatacctat
     7921 tgcaagtttg attttgtcaa tctttacttt aggtaactta ttacaatctt atagtgaatc
     7981 attacgtaat gtaattggat ttataggatt tattttgtat gcaatttata tagtcaaaat
     8041 aatattactt agaaagggtg ttaaagagca attagagaac actttaatat caagtacatt
     8101 tccaactata acaatggcaa caatgatatt tgctacatat ataaaaccat tatctttaga
     8161 attaagtatt atgatttgga gcataggaat tgtgggtcat cttgtgttaa tagtcttatt
     8221 tagtaagaag ttcttagtta aattctcaat aaaaacggta tttccatctt ggtatatagt
     8281 atatgttggg atagttgcag ctagtgttac ggcaccagca tttaatcaac ttttgattgg
     8341 caaaattgct ttttggatag gatttgtatc ttatatagtt cttataccag taattttaaa
     8401 gagagtatgg ataatcaagg aaatgcctga accatcttta ccaagtatag ctatattagc
     8461 tgcaccaggg tcactattat tgtctggata tattaattct ttttcagata aaaataatgt
     8521 agtattatac ttgttattca tattatcaat tgtattttat attatagttt tgggatattt
     8581 accgaaatta ttaagattgc ctttttatcc gagttttgct gcatttacat ttcctatggc
     8641 aataagtgca atgggtatga aactaatgaa tggttattta actaaatcag gtcattttat
     8701 aagttggatt tcatatgctt ctaagttaga agagataatt gctacagtga ttattttata
     8761 tgttttagtt ttgtttatta tatatcttag taaatctgaa actacatcta aagctaaata
     8821 agaattatta aatatgtgta tttaatagga atattgatat aaatatatga agataagata
     8881 aaccatagaa acttaattta gcttaatttt attggaggaa aacaaatgaa aacaaaagaa
     8941 gaatatttaa aagaagttag aaacatatca gaagaatact ttagaaaagg agagtttttt
     9001 tgctctgaag cagttctcca gacaataaat gatgctttag gccaaccttt aagtccagag
     9061 ataactaagt tagcatcagg atttccgata ggacttggaa aggcacaatg tctttgtgga
     9121 gcaatatctg ggggagagat ggctttaggt atagtatatg gaagagttca tggtgaagct
     9181 atgaatccaa agatgtttga gcatgctaaa ggtttgcatg attatattaa aaaagagtat
     9241 ggtgcaacat gttgtagagc tattacaaag aaatgggatg gtgataactt tatgtctccc
     9301 gaaagaaaag agcactgtat taaaataaca ggtcaagtta cagagtgggt tgcagataag
     9361 cttatagaag atgaaaaact taatatcaaa tagtgtaata ttagttggtg aatttataaa
     9421 gttaaattta cttatagtat aagatacttg atattctcgt attgatagaa aaatttatag
     9481 catatgtatt taatgaaata tacttatatt tagagtgtgg tttaatttca taaatacata
     9541 tgctattatt ttaagtatat aagcaaattt atttaaataa atagaaacag ttaatttata
     9601 tgtacaaaac tatatatgat tagtgtaaat aaatacctat tttataatac tcaacataaa
     9661 gacaccagac atgattagta ttataccaat aaaaatagca agccattctt ttttgttctt
     9721 cttttcttta aaaaatataa tccctcctaa agtggctact atcacacaac tctgagagat
     9781 agaaaaactt attgtaactc ccaatgtatt agtcgttatg aacataccta aatttgctat
     9841 tgaccacaat ataccagtaa ttaaattgaa taaaacattt tccctatata caatctcttt
     9901 agaaaaatac ttgacaataa tagatgaagt tatcaacata ccaacagatt gaggtaatat
     9961 aattctatat ccttcaatta aaaatatttg attagttaca acatataatg ataaaaatag
    10021 agatgataat aatacaactc caacacattt taacatattt ttatctgtag attttttagt
    10081 ttgataattt gtgagaagta taccaagtat tatacataat attgataaag aaccaatcag
    10141 taacatttta gaactggtcc attcattaaa caagattaca gctaataatg tcgtaaaagt
    10201 taattgtgaa ccatttgaaa ttggcattac tttagataca ctagcaattt gaattgcttt
    10261 aaactgcatt aattgtccca aactccagaa tatgccagaa atcatactta tacaaaatga
    10321 aaaaaatgta atttcaggat gtataaccag agttattata gtggaaaata taaacgcact
    10381 tatggatgta cctaaaagct gttcatgagg agaacttttc tttagatttg caactatggg
    10441 catcaatccc catcccatta caggaagtaa agcaataatt attgtcagca ttaaactatt
    10501 tcctttcata ttaaatttag acagctttta atttatcatc taaaattaaa gtgttcaata
    10561 cataaaaatt accgtattta atcaggaaat attagtagtt ttttagaaaa acattttcca
    10621 ttaaaataaa gacaaaggga aggagtaaaa ttgaaaatgt taactgatat gattcaaaat
    10681 cgtgaaatta aaataataaa gtttttatta aaagaaggtc atacaactgt aaaagcaata
    10741 gcagatgatt tagatgtttc agaaaaaaca gtttcaaaat ctcttaaaga agtagaagca
    10801 gtgcttaaaa agataggtct ttctttaata agaaaaccta agataggtgt ttatattgat
    10861 ggagatttaa aaaaaatact tccttaccta gataataaag gaagtcaaat tcctacaacc
    10921 agagaggaga gagttatcta tatatattca gtacttttaa agagtaatga ttatattact
    10981 atacaacaac tttctgatga actatatata agtcgtggaa ctattgaaag ggatttagca
    11041 gaagttgaaa aactgctaaa aaacgaaggg atttccatat ataagaaacc tagtaaggga
    11101 ataaagttaa atataagtga aagagataaa agacaactta ctgctaagtt tatacataaa
    11161 ttttggagta gaaactggta tattaagcaa gagcaagaaa gcttttatca agcatttgaa
    11221 aagatacaag cagatgtaaa tggtattttt tcggaagatg atttatcaat aattattgat
    11281 attcttagga atttcagcag agaaagaaaa tttgagttct ctgattatga atttcagtca
    11341 ttagttattc atttggcaat agctgtagag agaattagaa aaggcgaata tatagaagaa
    11401 agtttgtata ctggtaatat agaaaataat caaatgtcaa ataccaatta tttagcaaaa
    11461 caattagaag aaaaattttc aatattatta ccaaaatctg aaattggata tataaattta
    11521 catttagtag cgtctgacca acatagagat actaatgaac taaaaaatac ttcaactaat
    11581 ccttgtgaga caagtacaaa taatatatca aatcttcggg aaattataaa aaatacattg
    11641 tatgagacag cttatgacaa ggatttaatt gatggattga cttcacatgt acattcatca
    11701 ataaatagac tgaaatatgg acttagtatt aaaaatccat atgtaaatac aatcaaacaa
    11761 aacttttctt tatcatttga gcaagcttta gatttaaaga aagtaattga atttatatat
    11821 gatattagga taaatgatga tgaatgtgct tatatagcat tacattttga agcttttaga
    11881 gaaagaatga aagaacttcc acaaaatatt agagtaattc tagtatgcag tacaggatta
    11941 ggttcatcta ggttattgtc tgcaagaata aaaaaatact ttccaatgat aaagattgaa
    12001 aaagtacttt ccgtacaaga attagtatcc agtaaaattg atgcagattt agtaataagt
    12061 actatttatt tagagttaga aacaattcca accatagtag ttagtcctat tttaaatgaa
    12121 agtgatataa gaaccttgga aagaaatata caaaatgttt caagttgtac ttctaagaag
    12181 tataaatctt tcagaaactt aatctttgaa gaaaatatat tgttgaatat tgaagcttca
    12241 aactatgaag atgcaattaa gtatataaca gataacctaa taaaaaaagg ttttgcaaaa
    12301 gaaggtatag ctaaaagtgc aattgagaga gaaaatttat cttacacttc tttccaatca
    12361 atagctacac ctcatgctaa tcctagttat attaaaaaat ctaatatatc tgttgcaacg
    12421 ttaaaaaatc ctattacatg gggaaatgaa aaggtggaag ttattttctt tatttcatta
    12481 gaaaatgaca aaaacctaga gcttgatgaa atatatgaag ttttttatga ctttattgac
    12541 aataaaaaaa atataaatga catattaagt gcaacttcca caaaggaagt gtacgatttg
    12601 ctagtggagg agagtatata gtgaaaatat cagatgtatt gaaaaaagag caaattgttt
    12661 ttaatcttga tacaaataca aaggaagaaa caataaaaaa attaagtagt atatttgttg
    12721 aaaatgggat tgtggacaat gaagaagaat atgttaagtc tgttttggaa agagaagaac
    12781 attcaactac aggtattggt aatggtattg ctattcctca tgggaaaagt aatgctgtca
    12841 aaaaatcttc tataatattt gcaaagacta agcataagat tgaatgggat gctttagatg
    12901 aaaaaccagt agatagtatt ttcttattgg ctataagcaa tattgataag gatagcaatc
    12961 atcttgtact attatctaaa ttagcaacta aacttatgga tgatgacaat gtagatgcac
    13021 taaaattagc caattcagaa caagaaataa tcaatatatt tagtgaagaa ggagagatgt
    13081 agaaatgaaa agaaaaatta tagcagttac agcatgtgca acaggagttg cacatacata
    13141 tatggcagct caagcattaa agaaagcttc aaaaaagatg ggtcacctta taaaagttga
    13201 aacacaagga gctacaggaa tcgataatga gttaactaca aaggatgcac aaattgcaga
    13261 agtagtaata tttgcagtag atactaaagt cagaaatgct gaacgttttg agggaaaaga
    13321 aatactagaa gtaccagtta atgcacctat aaaggatgca gagaaagtaa taaatgatgc
    13381 attaaagtta attgacacta aataaatgga gggaaatgta atgttgaaaa agatagtctc
    13441 tgagttaaaa aagcatgtct taactggaat ctcatatatg attccattag taattgcagg
    13501 tgctatgata atggcgatat ctcgtgtagg tggttctttc tataatatac cagatatatg
    13561 ggatgctaaa tatgcagaaa gtgctagtag tattgttaga ttattacatg atttagatgg
    13621 gtttggaggt actgcattag gattaatgtt tcctgtaatt gctgcattta taggattttc
    13681 tatagctgat aaattagcta ttgtaccagg tcttgttgga ggtatgattg ctaaagatat
    13741 aggtgcagga tttttaggag ctttggcagt tggtcttata gcaggatata catgtttgtt
    13801 tattaaaaat catgttaaat taccaaaatc agcagcttca atagtaccaa tatttatagt
    13861 accagtattt gggacattaa tcactgttgt attaatcaat tatgtgatag gtataccttt
    13921 tgccacatta aatactggat tagaaaattg gttaaatgga ttatctggta caaatcagat
    13981 actaatggca gctattatag gggctatgat ggcagttgac ttaggtggac cagttaataa
    14041 agcagcttta acaacatcac ttgcattatt aacaagtgga atttatgcgc ctaacactgc
    14101 tgcaatggta ggaatagtta taccaccact aggattggga cttgctacta tactagcaaa
    14161 acaaaaatat aataaacagt tacgtgaagc tggaaaatca tctcttataa tgggtttaat
    14221 tggagtaagt gaaggagcaa ttccttttgc ggtagaatct ccattaaaag taataccttc
    14281 atgtgtaatt ggaacagcaa ttgcttcagc tatggcagtt ggtttaggtt cagtaaatgc
    14341 aactccaatt agtggatttt atggatggtt tacagtagag aattggccaa tgtatgtgtt
    14401 atcaatagca gttggaacag taattgtagc tggattagtt atagtattgc gtggaaatcc
    14461 agtgattgag gatgatgaat ttgaagatga tgattttgat gaagcagagt gggaatctta
    14521 aaatttaata actatttaag acgtgttata agctatattc aatatgaatc ttatactaaa
    14581 taaagtgtta aatattcagt ttagacttaa atataaaaga taaagaacag aggagaataa
    14641 atgataaaag cacatattgt taatcataca cactgggata gagaatggta ttttacttct
    14701 ggagatgcat tagtacttag tgaacaacta tttacagatg taattgatga attagaacgt
    14761 aatccagatg taagttttgt acttgatgga caactttcta ttttggatga ttatgtacaa
    14821 ttacacaaag aaaaaataga aatcattaaa aagttaataa aagaagatag actacatatt
    14881 ggaccatggt ttactcaaac tgatgcattc tttgctagag gagagtctat tttaagaaac
    14941 ttaatgattg gaatttttga aagtaaaaag tatggtaagt atatgaaaat tgggtatttg
    15001 ccagatacat ttggatttaa tgcacagatg cctatactat taaagcatgt aggtcttgat
    15061 aatatattct tttggagagg tatacatctt ggtaaacagg ttcaatcacc atattttaag
    15121 tggaaaagct taaatggaga aagttatata tatgcagtaa atatgccaca tggatatgga
    15181 actggtatgc ttttagagcc ttcattaaag tatgttaatg gacgcttaga cccagctatt
    15241 gattttatta aatcatatac tgatgtagat gaggtgttaa taccttctgg gaatgaccag
    15301 ttaaatataa taggtgattt caaaaataaa attaaagcga ttaatgatat cggaaagtat
    15361 tcatatataa cgagttcata tcaagagttt ttaaaatata taaagtccat agaatcacta
    15421 gaaagctata gaggtgaatt tagagaacct gtattagcaa gaatacataa atcaattggt
    15481 tctagtagaa tggatataaa attagcatgt gataaactag aaaacaagct gattaaaaga
    15541 atagagccat tattagtaat tgctaaaaag agcaaaattg aaataagcaa tcagttactt
    15601 ataaatacat ggaaaaaact tttggaagga caagcacacg atagtttagc tggatgtgta
    15661 actgatactg taacagatga tattcttcac cgtataagag aagcaaatga aatatgtgac
    15721 agtattgaaa atactattgt aaaaaagatt tcagaagggc ttaaattatc taaaaatgat
    15781 attttagtat ttaatacaga agctaagcgt tttaatggat ataaagagat acaagttgta
    15841 agtgatagta aaaatattta ttttaaagat aatttagatg ctactataat tgaagaaact
    15901 tatgtaaaac cgagagaaaa tgtattagaa gaaacacctg ctggtaatat atttattgaa
    15961 gaaccaggat attatatatt aaaagtaaga ataaatgtac agcttcctgc acttggttat
    16021 aaagttgtta gttttgaatt aagtgataaa gaaatggtgt cacttgataa atctaatgac
    16081 acttttatat ctaatgataa ttataagata atatatgaag atggaagctt aaatttacaa
    16141 cttaaagatg gaacttacat atcaaacttt ttaacattaa aagatagtgg taatgcaggt
    16201 gatacctatg acttttctcc actaaaaaat gatgagaata taaaactttc gtttgataaa
    16261 gtgtatacag aaaaagcttt aggatatgaa aaaatggtgg ttaaaggtac tactttacta
    16321 ccacttacat tagaagatag aaaaaacaaa tatcttaatg gtaaattaaa tgtaaaagta
    16381 agtattgtct tatctaaaga aaacccattg atggatgtaa aactttgtgt tgataacacg
    16441 atatataatc atagactacg tgttcatata aaaactgata taaaagataa taaaaacata
    16501 gcatcattgc catttggata tataacaaga aaaaatggag ttttagacaa ctgggaagat
    16561 atttacagtg aaatgcctat agatttagaa cctttggaaa gtaatattac gcttacaaac
    16621 caaaatagaa gttgtactgt atttactaga ggaataaaag agtatcaaca tattgaagat
    16681 gaaattgcat tgaccttact tgctactaca gatgaactag gaaagcctga tttattatat
    16741 agaccaggac gtgcttctgg tgatacaact aaaaaaggtc atataagaat taaaacagat
    16801 aaagctcaaa tgcttaagga attagaattt tcatttgcaa tatatatgga ttctaaatca
    16861 tttgatgaat tggaaattgc gaatttaacc cacaattatc taagggaatg tgtaaattat
    16921 caattacagg attacaattt ctttttatat catatagata ataagattca aaaatcaatt
    16981 gtcaataaaa ctgtaagtag agaattagaa ataatatctt taccagaaaa ctatcttata
    17041 tcagcatgtt atccttctta ttacagtaaa gataaattta taataagagt ggaaaatcca
    17101 agtagtaata gaatgatatt ggatgagcaa atatttaaaa atagaaatgg gaaaattgta
    17161 aatgcaatag aagatgtaga agaggaacag gtatatgaaa tagcaccttt tgacgttatt
    17221 tcaatattat tggatttata agtgtaaaag taagtttatt gtttaaagtc tgacagatat
    17281 taatctgcca gactttttaa tttgtataaa tttgggtaaa tattaatgaa tggttttagt
    17341 atttaagtat gaatttagta tttaatatag aatttattga agtagaataa ttagtttaac
    17401 aggataagca gtttaaataa agaattatta tattagagtg attttatata taatttaaaa
    17461 taataaaatt ttaa
//
LOCUS       DAMJWS010000089        17327 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMJWS010000089 DAMJWS010000000
VERSION     DAMJWS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.090"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            124..486
                     /locus_tag="PC399_003053"
     CDS             124..486
                     /locus_tag="PC399_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF2593898.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            922..1623
                     /locus_tag="PC399_003054"
     CDS             922..1623
                     /locus_tag="PC399_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF2593899.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1893..2711
                     /gene="larE"
                     /locus_tag="PC399_003055"
     CDS             1893..2711
                     /gene="larE"
                     /locus_tag="PC399_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF2593900.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2766..3914
                     /locus_tag="PC399_003056"
     CDS             2766..3914
                     /locus_tag="PC399_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF2593901.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            4050..5438
                     /locus_tag="PC399_003057"
     CDS             4050..5438
                     /locus_tag="PC399_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2593902.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            5467..6165
                     /locus_tag="PC399_003058"
     CDS             5467..6165
                     /locus_tag="PC399_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593903.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            6168..7601
                     /locus_tag="PC399_003059"
     CDS             6168..7601
                     /locus_tag="PC399_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2593904.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            7623..8360
                     /gene="truA"
                     /locus_tag="PC399_003060"
     CDS             7623..8360
                     /gene="truA"
                     /locus_tag="PC399_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2593905.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    8471..8678
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8931..10130
                     /locus_tag="PC399_003061"
     CDS             8931..10130
                     /locus_tag="PC399_003061"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF2593906.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            10377..11423
                     /locus_tag="PC399_003062"
     CDS             10377..11423
                     /locus_tag="PC399_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF2593907.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            11531..12694
                     /locus_tag="PC399_003063"
     CDS             11531..12694
                     /locus_tag="PC399_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2593908.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12783..13163)
                     /locus_tag="PC399_003064"
     CDS             complement(12783..13163)
                     /locus_tag="PC399_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF2593909.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(13166..13825)
                     /locus_tag="PC399_003065"
     CDS             complement(13166..13825)
                     /locus_tag="PC399_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF2593910.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13931..14905)
                     /locus_tag="PC399_003066"
     CDS             complement(13931..14905)
                     /locus_tag="PC399_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2593911.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14945..15292)
                     /locus_tag="PC399_003067"
     CDS             complement(14945..15292)
                     /locus_tag="PC399_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593912.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(15510..16466)
                     /locus_tag="PC399_003068"
     CDS             complement(15510..16466)
                     /locus_tag="PC399_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF2593913.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            16909..17277
                     /locus_tag="PC399_003069"
     CDS             16909..17277
                     /locus_tag="PC399_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2593914.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         6842 a         1807 c         2805 g         5873 t
ORIGIN      
        1 gtttaataat ttgaaatatg ataatttttt atgttgaaag cttataatga ttatgatata
       61 aattggttat agtatacaat ttatgggtat aacaataaaa aattagaaag gaatttttat
      121 aaaatgatta aattatatgg atatacaaaa tgctctactg ttaaaaaagc taaaaattgg
      181 cttaaggaaa ataatttaga atttgaagat atagatatgg ttcaaaaccc tcctagtaaa
      241 gaggagttaa aatcaattta taagactagt ggatatgata taaagaaatt ttttaataca
      301 agtggtatga aatatagaga attgggtctt aaagatattg taaaaactga atctgatgat
      361 aaacttttag aaatattggt aagtgatggt atgcttatta aaagaccatt acttttggat
      421 ggtaaaaatg tattattggg ttttaaagaa gatgtatgga aaagtacttt gctaaaagaa
      481 gattaggaag agataggtgt gttgattaaa attattaatt aatacaccta taaatttatg
      541 tattaaatct aatctttata aatattaatt agcaaattca aatcaaaaaa attcatatca
      601 acaaacatat tacttcttat tacaaattat atcatatgtt ttatattaaa atattataat
      661 attacaacga attgttacaa aattacttat tattaaccat gaacatttgt tttgtgctat
      721 catatttata taagggaatt gtcataaaaa atatcttgtt catacagttt tatatagaaa
      781 aaagaaatga atcttttctt tatataagta tattttgtaa attgcctcta tatttatagt
      841 ttaaataata gtggtattaa aaatctgaca acaaaaataa aactttcaaa aaattaaatc
      901 taaaaagaat aggagataaa tatgagtatt gaagatttat atgattttga atgtgttcct
      961 ttgaacaatg agattgatat tgatgtttta tttgaaaaga tgatagaaga tggaaaagaa
     1021 aaagaatata caccaattat agtcattgat gaaaaagtag ggctattaag aaaaaatata
     1081 gatatgatta ctaaagagta tggttctctt gaaaactata gagagtattg tttaacaaaa
     1141 tataatgaaa ttgatgtaaa tcaatttttt gattttagga agagtgagat ttcagaatta
     1201 ttaaaggagc taattgattt agaagatgat ttttatgatt ataaacattt tagacccatt
     1261 atgaatatag atatatttca tgaatataac aaagtttaca tagctaaaat tccaacaatt
     1321 aaaccatatg aagtatttgc atacatacct attggcggat ttaatgattg tcctagagat
     1381 gaagaacata tagctattgc aaaatattgg tatgaaaagc atggaatctt tccaatcgca
     1441 attggatgtg atacagttca atattctgta aaaaatttaa ctaaagatgg taaaaaattt
     1501 gatgacttat gtattgaatt agtattttat tgtgaggata ttattataca agggtatgaa
     1561 accttaaagg ctcttaagga tgtattaaaa cgctctacaa tagtgctttt ttggtgggat
     1621 tgatattcta tcatttagat agtattaaat tgttagtcac caagaatcca cttgctttag
     1681 ttatagggag tgtgtcaaac taggttttta tacacagtgt agcatagtaa tatgtttaaa
     1741 ttacaaaggt taaaaaaata ctttaatatt tttaatatta taattaatag atttaaggta
     1801 attataattt ataaaataaa tactttatat gttataatca ttaagaatgt aaaaaaataa
     1861 tatatagttt tatagttcat atttttccag atatggaggt aaattttgtg aaggaaaaag
     1921 aaaaattaga taaattaaaa aaaatgttac ttgaattagg cagtgttgta gttgcttatt
     1981 ctggtggagt agatagtaat tttttgctta aggtcgcaaa agatacatta ggtgaaaatg
     2041 ttatagctgt gactattcat gcaatgatgc attcaagtag ggaaattgaa gaggctaaac
     2101 aatatactca aaattttggt gttaaacata tcatattaaa tatagaaaac tttgatttaa
     2161 aagaatttaa agaaaatggt gttgatagat gttatcattg taaaaaatat atattctcta
     2221 aaataaaaga ggtagcaaaa gaacataata taaagtacat agtagatgga acaaatatag
     2281 atgatttagg agactataga cctgggctta aggcattgag tgaattagga gtaattagtc
     2341 cattaaaaga tagtagttta aaaaaagaag aaattaggtc gctttcaaaa acattaggat
     2401 taaatacttt taataaacca tcatttgcat gtcttgcgag taggatacct tatggagttg
     2461 aaataacaga tgaaaattta agaatagtag aaaagagtga agaatatcta tccaatttag
     2521 gtttttctca atttagagtg agaatgcatg gagatatagc tagaatagaa gttggacagg
     2581 aagagctagg taaatttttt gaaaataaca attttaataa agttgatact aaattaaaaa
     2641 tatttggctt taaatatgtt acactagata tgtcaggata taaaatggga agcatgaatt
     2701 taaatgtgta gattatttta aaaataaata agttttatag ttaatataag gaaacaggag
     2761 gttttatgaa aaattatacg ttaatatcgc catgtttttt tggaatggaa aaaatgctgg
     2821 ctagagagat tactaattta ggatatgaaa taataaaaac agaagatgga agaataacat
     2881 ataaaactga tgagtttgga atagcaaagt caaacatgtg gttgagatgt gctgaaagag
     2941 tacatcttaa aattgctgaa tttgaggcaa aaagctttga tgaattattt gaaaatacaa
     3001 aaagaataaa ctggtctaga tatatacctt atggagctca atttcctata tcaaaagctt
     3061 cctctataaa atcaaaatta tacagtacac cagatgtaca agccatagta aaaaaggcta
     3121 tagtagaaag cttaaagaaa agttatttgg aggatggttt gcttaaagaa gataaggaga
     3181 aataccctat ttttgtattt atacacaaag ataaagtaac catttctata gatacaacag
     3241 gagatgcttt gcataagaga ggatatagag aaaaggcaaa taaagctcca ataagagaga
     3301 ctttagctgc tgggcttatt tatttaactc cctggaaagc aggtagagtt ttagtagacc
     3361 ctatgtgtgg ttctggtact atattgattg aagctgctat gattggtata aatatggcac
     3421 ctggcttaaa tagagagttt atatcagaaa agtggagaac tcttgataag aaaatatggt
     3481 gggatgtaag aaaagatgct tttaataaga tagataatga atctaaattt aagatttatg
     3541 gatatgatat agatgaagaa tctatagata tagctagaga aaatgctgaa atagcaggtg
     3601 tagatgaata tatagagttt aatgtaggag atgcaactca atttaaaagt gaagatgagt
     3661 ttggatttat aatcactaat cctccttacg gagaaagatt agaagataaa gacagtgtta
     3721 aacagttgta taaggagctt ggttatgcat ttagaaaatt aaaaaattgg tcgtattatt
     3781 tgataacttc ttatgaagac tttgaatatg agtttgggca aaaagcagat aaaaaaagaa
     3841 aattgtataa tggtatgtta aaaactaact ttttccaata tccgggtcct aagcctccta
     3901 gaaataataa ataagtttta catgatttat ttgtaaaata taaaatttta ttaaaataag
     3961 taaaacatta tgatgccctg aaacgtctaa ttattgtgag gagatttttg tgaacaaatt
     4021 taaagattta taggattagg aggagttaaa tgaaagtgtt aaagcttatt actgtagtag
     4081 ccctgatgat gttatttgtt actggatgta atataaaaac tgaatctcca gaacaattag
     4141 cgaagatacc tgactatgat gatacaaaaa aagtattgta tgatggtatt gaccagttac
     4201 taaaaccaga ttcatctatg atattgcctt ctaatacaaa agaagtagga aaaataaata
     4261 aggtagattt aaacagcgat ggaattgatg aattagtagt atttgaacaa aaagaagatt
     4321 taagtaataa tgtgagtcag gtagggtttg taaccttgag ttacaatgga gaaaaatacg
     4381 tacttggaga ccatttttta gaaaatggcg aatcaataga atatgctaat ttttatgatt
     4441 tggactcaga tggatataag gaagttatct tattggttaa atccaaagac aaaacgaata
     4501 tgcatatata taaagtagta gataatgaaa taacaaaaat atatgacttg gatgcatcat
     4561 ggcttcaaaa taaagaagat tttaatgaca tgaaagtaaa aattggatat atagatggag
     4621 atgacaagtt agacatattg atgcttcatt tgaataataa gactaatgaa atgtttgcaa
     4681 gtgttgcaaa ttttgatggg aaaatgaaaa tcaaagattc tgttaagttt gaaaatgtaa
     4741 aaaacttaag tgaattatat ataacattag gtaatgttgc ttcatctgtt ggaaattctt
     4801 ctgcagctat aaaaggtatt gtacttgata ttcctatatt gaaagataat aactatatta
     4861 cgcagatact ttatatgaaa gatgggaaaa ttaacaaagc ttttagtgat tatgataaaa
     4921 ctattactaa atcttattat atacctgtag atgatattga caaagataag attatagaaa
     4981 tacctatagt tgcaggcagt acaggtaata ataaaaatac ttatagttct aaatcatctg
     5041 caactataag ttggtataga tggaatggaa aagaaggtgc tgactcaagt ttaatattta
     5101 caagtcagat atattataat tataaatata attttaaatt atttattcca aataatttat
     5161 ttgataaaat acttgttgaa caggaatttg ttggagaaaa agcagtattt aaattttact
     5221 attttgacta ttcagaccgt aagaatttat ttaatatagt agtagagagt aaaaataaac
     5281 ttgaagatag aaaaaataca ggtactcaaa attctattgt acttcaagag agcgatgaat
     5341 atactttctt actagttgta aacaatgcaa atgaaatgga taagctagat atgactatag
     5401 acgcattaaa agaatatttc tcattaatat atggttaata ttatactcaa ttggaggttt
     5461 tattttatga aggaaaagat acttatacta gaagatgaaa ttggtattag aagttttgtt
     5521 agtataaact taaaaaggga aggctatgaa atagttgagg caggtactgg aagagaagct
     5581 atagaaaaaa tgacaacaga aaaggatatt acaatagctc ttttagatgt aatgcttcca
     5641 gatataagtg gtatagaagt atgtaaattt ataagggaga attttgacca agtaggtata
     5701 ataatgctta ctgctaaagc tcaagaagat gataaaatag aaggatttat ctcaggagca
     5761 gatgattaca tcataaaacc atttagtata aaagagcttt tagtcagagt ttctgctctt
     5821 cttagaagag tagcaaagga tgattcaagt gttaagtcga gtgagatagt ttctccacca
     5881 tttatacttg atatagacaa aagaaaatta tttaaaaatg gaaaggaaat tgagcttaca
     5941 ccaactgaat tttcaatagt gaaatatcta atatcaaatg ctaagcagtc gttaagtaga
     6001 gaccagatat tagacgaagt atggggaact aactatttat atgattttaa aatagtagat
     6061 gtaaatataa gaaggataag aaataaaata gaagatgacc catcaaaacc aaaatatata
     6121 caaactattt ggggttatgg atactgtttt agaaaggaag aataatagtg ttacattttg
     6181 aaggtctgag aaaaaaagta ataaaaaact attttataat aattattata atggttactc
     6241 tgtttgaagg attgttcatg ttttacatac aaaactacta ttatgattct gtcaaacagc
     6301 tacttgaatc tgaaataaaa tatgctgatg aatataatgc tattacaatg gaaactacaa
     6361 gttttgagaa aaaagtaaaa aatatatttg ataaacaacc cttaactaaa aattcagaat
     6421 ttggaatttc aataatagat aaagataaaa atattatatt agaccaatat ggttttaaaa
     6481 gtaaagaaaa agcaaactat gaagatgtta acaatgcatt aaaagatata aaaactaaaa
     6541 atttaacacc ttatacttat agaataccag atacagggga gcatgttatg agtatctcct
     6601 taccacttaa ggtaaataat ataatagaag gtgttgttag atatacagta tcattagatg
     6661 ctattgataa tgcaatatta aagcaagcta catggcttat attagcagga atatttatac
     6721 ttattatagc tatactaata agtcttaaat ttgctgagac tcttataaaa ccattacgag
     6781 agctaaagaa atttgcaaat gaattggctg taggaaatta taatataaaa ttagaaaaga
     6841 tgaagattgt agatgatgaa attggtgatt tagcacagac atttgaacac atggcacatg
     6901 agattgacaa aagtgaaaaa ttaaaagagg aatttatatc atctgtatct catgaactaa
     6961 ggactcctct tacatctata aaggggtgga gtgaaacctt aggttatgaa agtataacaa
     7021 gagaagaact agacttaggt ttaggcatta tacaagatga aacagaaaga cttataaaac
     7081 tagttgaaga attattagac ttttcaaggc tttcttcaga caggataaaa cttcatgttg
     7141 atatagttga tgttgaaggt ttaattgtag gtgttgtaaa tcaacttaaa gttaaagcag
     7201 ctgaaaaaga tatatcactt ttatttgaat ttgaaaatga atttatagag aacattcaag
     7261 gagataaaaa tagattaaga caggttctca taaatttaat acaaaattca tttaaattta
     7321 cgagtcaagg tggttacata aaagttgtag cttctcaaga tgaagagata acaaccatat
     7381 cagtagagga taatggttca ggtatagaaa agcaaaattt aaataaagtg ttagataaat
     7441 ttttccaaga agattataat aaggctggta gtggattagg actagcaatt agtaatgaaa
     7501 ttgtaaaact tcatggtgga agaatgaaaa tagaaagtga aaagaatgtt ggaacaaaaa
     7561 taacttttaa tataaaaaat aaatttgcta aacaagcata aattcttaga aagaggatta
     7621 agatgagaaa cataaaaatg atagtagcat acgatggttc aagatataaa ggatatcaaa
     7681 aattaggtga caataatatg actattcaag aaaaacttga aaatgtttta agtaaaatga
     7741 ctaatgaaac agtggagata ataggttctg gaagaactga tatgggagct catgcaagag
     7801 gacaagtggt aaattttaga actaattgta tggattcttt ggataaaatt caaaaatatt
     7861 tatatgaata tttaccagaa gatatagttg taaaaactgt tgaagaagtt gatgaaagat
     7921 ttcattcaag atacaatgta aaatcaaaga cttatatgta taaaattgac aataataagt
     7981 atcataatcc ttttataaga aaatatgcta ctcatgtaag taaaaaatta gatttagata
     8041 gaatgagaaa agcaagtgag tatttagtag gagaacatga ttttactagt tttgcttctt
     8101 ctaaatctaa gaaaaaatca aatgtaagag aaatttattc tataaatata aaagaagatg
     8161 ataatgttat agaaatatat gtagaaggaa atggattttt gtataacatg gtaaggataa
     8221 tagtaggtgc tttgatagat gttggtctaa aaagaagagc tccacaagat attaagtata
     8281 tgcttgaatc aaaagataga tgtcaatcat cagatactgc accagctaaa ggactatgtc
     8341 tttggaaagt aacatattaa tcaattatat aaaactctct ttaaaataaa aataaaataa
     8401 attatatagt tgacataaat ggaaaaataa actattatat attttataag agagatataa
     8461 aactttatat ttaaatcctg tgagaaagag agtaacttct atgtgtttta tagagagctg
     8521 aggatggtgg aagcttggca aataagtatt agtgaaaaga actttggagg atactaattg
     8581 aatttagtat gttagtaggt aatctcgcct taagagaaag agtggataag tttatcttat
     8641 caattagagt ggtaacgcgg atatatttcg tctcttggtt tttccaagag gcgtttttta
     8701 atatatttaa ttattagtgt ttatctggta tgttgaacat ttactaattg attagctaag
     8761 tttatcaata agagtggtac cgcggatatt tcgcctctta gtattttaaa atactaaggg
     8821 gtttttttag tattaaaatg attttatgtg agtctaaatt ctcagagtca ttcaatatta
     8881 agaatttcta taaaataaaa attataattt aagattggga ggaattaaaa atgaaggaaa
     8941 aagtagtatt agcatattca ggaggacttg acacatcaat cataatacct tggttaaagg
     9001 aaaattatga agatatagat gttatagctg tttgtggaaa tgtaggtcag gaagataaaa
     9061 tggaagatgt atatgaaaaa gctttacaaa gtggagcatc aaaggcatat gtggatgata
     9121 taagtgaaga atttgtaact gaaactatat ttaaagctgt aaaagcagag gctaaatatg
     9181 aaggaaaata cttattagga acttctttag caagacctat aatagctaaa aaattagttg
     9241 aagttgctca taaagaaggt gcaaaatata tatgccatgg atgtacagga aaaggtaatg
     9301 accaagttag atttgaagct acaattgcag ctcttgaccc aactataaaa gttattgcac
     9361 cttggagaat ttgggatata aaatcaagag aagatgctat agactatgct gaaaaacata
     9421 atataaaggt aacagcaact aaagctaaaa tatattcagt agatgccaac ctttggcatg
     9481 tttcaacaga aggtggagat atagagcacc tagaaaatga acataaaaaa gatgtttata
     9541 aacaatgtgt agacccagaa gatgcatgtg atgtagctga atatgtagaa gtttactttg
     9601 aaaaaggtgt acctaaaaag gtaaatggcg aagaattatc tccagttgca ctgattcata
     9661 agttaaatga gttgggttgt aaacatggaa ttggtgttat agatatagtt gagaatagac
     9721 ttgttggtat gaaatcaaga ggtatatatg agactccagg aggaacaatc ctttatgaag
     9781 cacataatat tttagaaagt gctactttag acaaagatac attacatttc aaacaaatgg
     9841 tatcttataa atacggagaa cttatttaca atggactatg gtattgtaaa ttaagagaat
     9901 ctatagatgc atttatggaa caaactcaag ataatgttac aggaacagta aaagttaaac
     9961 tttacaaagg aaatataaaa cctgctggaa tatttactga aaatgctctt tatgatgaag
    10021 gaatatcttc ttttggaaat agtgaacttt atgaccacaa agatgcagaa ggatttatta
    10081 atttattcac tttaccatta aaaatcagag ctatgaaagc tggaaaataa taaaataaat
    10141 tatttaagag tgtataaaaa cgatttttag gtttttatat gctctttttt tatttgaatt
    10201 tattaaatta tacagtaaat agtataaaaa aatatattat agttccaaat ggcttaataa
    10261 tgtgaaaata taagacctaa aatataaatt ttataaatac aatagttatt gactctctca
    10321 ttgtaagagg gtttatactg aatctaatga aaatttatag tgaggagata gattatatga
    10381 gaaaaataat tgaaattaaa catcattcac atgattatga gtgtatgtgg aatggtatag
    10441 aagatttata tataaataaa accggtgaat accttccaaa cgattttttc tttttactgt
    10501 caggttttgg ttcattttgt tatatgaaaa caaataaagc tgatttgaaa agaatggtag
    10561 ctttgggaga tggaaggaca aaacagatgt ataaattttt agcaccaatt gttggatttg
    10621 aatacaaaca tcatgagttt aaaaagtatg agcaagcatt aaagaaagca aaatctgaaa
    10681 ttgatgatgg atatccagtt attttaggag ctttagatat gtattatcta ccatattata
    10741 agaagctata tcataaagaa catattcctt ttcattatat attaatggta ggatatgatg
    10801 atgaaggaat atatctgtat gattgtggta gaacagaatt attacatctt tcttatgata
    10861 aattacgtga aagcatgaat tgcaattccc ctggattaag caaagaaaat actatttgca
    10921 caataagaat gaatacttca aagaataaat ataacattgc tagagaagct attgctataa
    10981 agcgtgatat gtttttaaat ccacctaaag gatttttggg atataaaggt tttgaaaagt
    11041 ttattaaaga gttgccacag tggaacactg aattggataa ggatgattat gataagattt
    11101 tgaccaatat ggttatgttt tttggaacag taccaacaat tccaaatgca atcaaaggaa
    11161 ttaaagaacc agatactgtt caatttaaag gtggatttga taaaatgagt agaatgctca
    11221 acagtattgg ggaggaatac aaaaacaact cttttataca agtagctgat atttttgata
    11281 aaactgctat tataattgag aaaatatcca atattataat agattactta actcaaacat
    11341 gtgatgatac agaacaactt ccgttattgt tctctgaggt cttagagcat atgaaaacag
    11401 gatatttaat cttggatgta taaaaaatat attgttgacc ctaacattac gtaatagtgt
    11461 aatatgaaaa tatagcttaa atatattagt aggtattgat ggctgtaaaa ttaaaatttg
    11521 aggtgtggat atgagaacag tgaaacaagt ttctgattta actggaataa gtgtgagggc
    11581 attacattat tatgatgaaa taggtctttt aaaaccaaat aaaattacag atgcaggata
    11641 tagactttat gatgatgaat ctattaaaac tcttcaacaa attttatttt ttaaggagat
    11701 tgatattcct ttaagagagg ttaaggaaat aatgtcaagc caatattttg ataaggttga
    11761 agctctaaaa aatcaaaaaa aattacttat tttaaaacga aagagactgg atgaattgat
    11821 agagcttata aatcaaactc taagaggaga aggaaatatt gattttaaag agtttgatat
    11881 gagcgagtat tttaatgttt tagaagaatt taaaagagag cataggaata aggtaattaa
    11941 aatttatggt agtgtagaaa aatacaatga atatatagaa agagttaagt ctaatgaaga
    12001 gaaaattgct aaaatggcta taaaacaata tggaactatt gagaagtttg ctaaagctat
    12061 aaaaacaaat tttagcagtg atatattgaa tttaggagaa aaatttgata gatataaaaa
    12121 tgattgttta aaagaaaaac atccaaaatt aaaagaatta tatagaaagc ttgtagaaga
    12181 tttaagtagg aatccttctt caacagacct tcaagaaata gcaaaagcga taacagatat
    12241 atctaaaaaa gattatgaaa ttttctctat ggatactggt gatgacaact ggtattatat
    12301 ggttcaaaat tatctagtaa atcctatgtg gatagaagaa gtagataaaa agtatggaag
    12361 tggagcagga aagtttattg gacaagtact aaagacttat ctaagagata gaaaaccaaa
    12421 gataaataca ttgtatgaaa aacttgtaga agatttgagt agagattgtt cttcaaggga
    12481 agttcaaagc attgttgaag aaatagacaa tgaaatgaaa agaagtaatg aattttataa
    12541 aatagacaat ggtgaaagat actttgatta tatgtcagag ctttatttac aagattctaa
    12601 ctatataaaa gtaactgata agaattatgg tgatggtgca tctaagttta ttggagaagc
    12661 ttttaagatt tattttgata ataataattg ttgaaaattt ggaagtaaaa aatatgtaag
    12721 ttttataaaa gcacaaaaat aaggatgtag attagctcca tctatatcct tatttaaagt
    12781 tattaaaata tatagtttac atcaaactta acactttcaa taatatttcc caaatatgtt
    12841 tttatacttt caacttcttc taaagatttt aatttatttt cagtataaat tgctttgtcc
    12901 agaatattaa accttaatcc tatttcatca aagtactcat gatttaaaac tttgtaccaa
    12961 tggttttttt ctttattcca actcgtagat attgaatcag cttctttttg agctttttcc
    13021 caatcttcat tttctataga tttttctaaa acagatatat tgtctttata tccttctgta
    13081 aaatcatata gtttattgtt aacgtaaaat ccaaataata caaataagat agtccatagt
    13141 ataacaaatg ttgtagattt caatattaat cacccttttt attttgaata aatattttgt
    13201 cattttcatc taaaacacaa ataagaacat ctttaaaagt ttctatgtga ttagatttta
    13261 aaatacccat taaccagtta gtatcttttt gagctatgtc tagattttta tttattattc
    13321 taccatccag tataagtggg ataggtaaat gattaaaagc tcttggagga gtactattgt
    13381 agcttgatgc tggtacaaca cttagattac catcagtctc caaaatagca tattgtacat
    13441 cttttaggtt aaagtatcct tgaatcctaa gttgttctaa taattcatct atactaattc
    13501 tttctttttt aagctcttta tatacaattt ttcctttatc tattaataca gaaggtttac
    13561 ctgataaaaa tgaacttaat tttctgcttt ttaatgataa gaatgaaatt agagtctgca
    13621 tgattacaag tccagatatt gctgcaacac catttatgat tggtatatta ttattttcca
    13681 ttgggacaga tgcaacttct gcaattaata gtgttataac taaatcaaaa cagttcattt
    13741 cagctatttc accttttccc atgactctaa gtgcaattag cactgttatg tataatataa
    13801 tacttcttat taatactact gtcatatcta tcaattactc ctttaaaaaa tatttacact
    13861 tagttagtat atcctatata aaaaatttaa ttcaaagtat aaaaaacctt aaataattgg
    13921 attcaactat ttaaggtttt tttgataatt ttaatattaa attttttatg gattttctgt
    13981 tacttttatt tattttaata acagattcaa caaaactctt tttatctcct aattttaaga
    14041 taaaatcagg atattcttca aattcattga gttttattaa tttgaaagaa gaagatagat
    14101 ttttaaagtt aataatttta gagttattca ctaatacact tcttttttta aaataaattt
    14161 cttttttact tatatttagt atagttaata tttgtttaag atatattatt atatcttctg
    14221 gagttctatc aaatttgagt tcaaaatttt catcttcaaa gtagggaata taatataaag
    14281 gtgttttgag agcaggtaat ttattatata attcttcaag aatatcgata tttttagggg
    14341 agttaaagaa aaagcattta catacattca ttaaatccac agcatctgaa agtgagttat
    14401 gtttaatatc tccttctaca gaaaaaattt ttttcatatt cagtaaagat atttgcttag
    14461 ataagattct attattgtat gtaatatgtt gagatataaa aggttgaatg tcaatcatca
    14521 tattaaataa ctctaagcat ttacctgtat atttatacac tctaaatgaa gttagaaggc
    14581 cagttatatc aaaatcaccc caattatata ttgtaacatc atcaaattta caaaaccatt
    14641 tataaaaatc atccataaca tataaaagat taggagcatt attgatatct agttggttca
    14701 atttggttaa atttttacat ttatttgaaa gtattttatt gtattttggc ttaactaaag
    14761 agtaataatt atctattgta ttaaattcgt tatcaactaa aactccacca atagaaatta
    14821 attccgcacc attatattct tttggtttat ttttattatc atctagtata ttaaattcaa
    14881 agtctataaa taacttgtat ttcataaatc taaagcacat cctcaggtaa attatttatt
    14941 gagcctaagc atcataagaa tcaatagata taatgactct aaacatcaaa ggttcattac
    15001 aacttttatt tatttcataa tttaattcaa ataaatcttt taagatataa tttataagta
    15061 ttctttccca acccattttt aaattaactt tagagtctat taatatattt tctatctttt
    15121 taaatatttc tatatttgct ggtattatac tgagctcaat attactttta aacgggttag
    15181 actgtaaatc tgttataagt atatttttta attcctctgt aagttcagaa attaaactat
    15241 cacctatgta tttttttatg atttcctctc taatttcttt taaatttagc aatattagta
    15301 cctgctactt acatagtagc ttttcctttc cttttaatac tattattcat ttttgaatct
    15361 tatttagata tattttaaga ttaaaagtct ttagctatat aattttttac ccatttttaa
    15421 cttatattaa catattacag gcttatatct ccaccctgtt tacattgagg atggagtata
    15481 ttgcctattt tgataaaaat gataggttgc tatttgccta ttcctttttc tgataatttt
    15541 tctaacatat ctttggtcat tttatctaaa tcataactaa acttaaagtt ccattctttt
    15601 actgcactac tactgtctag agagttaggc caagaatcag ctatactttg tcttacaggg
    15661 tctacatcat attctatcac aaaatcagga atatattttt ttatagaatc agctatttct
    15721 tcaggagaaa agctcatagc agtaatatta aaagcatttc tatggatgag tttatcagct
    15781 ggagcttcga ttaaatccac tattgattga agtgcatcag gcatatacat catatccatt
    15841 tttgtacctt ctgctatgta agatttatat ctcttatttt ttaatgcctc ataatatata
    15901 tcaactgcat aatctgtagt tcctccacca ggaggcgtaa cataagaaat aagacctgga
    15961 aatctaacac ctctagtgtc tactccaaat tttgagtgat aataatcaca tagtaattct
    16021 ccagaaactt tagtcacacc atacatagta ttaggtcttt gtaatgtatc ttgaggagtc
    16081 atattttttg gagaattttc accaaaagca gcaattgaac taggtgtaaa aaatttacaa
    16141 tccaattctt tggctatttc aagaccattt attaatccat tcatatttaa gttccaagct
    16201 tctagaggtt tactttcagc aactgcagaa agtaaagatg ctagatgaat tatagtatct
    16261 acatcatatt tttttgcaat ttcaaaaaaa cgattcttat ctgtaacatc taatatctca
    16321 aatataccag attcacatac tggattacct tcttttactc tccttgaact agcgattaca
    16381 ttttgttctc catactcatt tctaagcttt attgttaatt ctgaacctat ttgtcctaat
    16441 gcacctgtta taagtatttt tttcaatttt acaacctcct aagggtttaa tttatgtagt
    16501 actacttatt tttattttag caaatctttt gtttaaatac aaagaattaa agataataat
    16561 atacgactta attgaatgaa gtgcagtaag tgcgtaaaaa tttaaagcta atatataatg
    16621 gtctattgta taacaataaa agagtagatt aaattattat atatagattg tgtgactaag
    16681 tcacagaaaa tagagctaat ttaatatata ataaaagcat gtcaagtaaa gctttaaatt
    16741 aaagtaactt tatataaaaa gtaatataaa ttgataaaat tataaattta tttatagtga
    16801 atataggttg gattttatag atacaaaaat agtatatatg atatgtagac cattagcatg
    16861 tttaaagtag caaaacatat aaaataaaat ttgaaaggaa gtataaaaat gacaagacca
    16921 tcaatatatc atagccaagg atacacttgt gcagaggcat taataaagtc ttataatgag
    16981 gaacacaaca cagatatacc aatctcaata ggaagtggta tgggggttgg aatgaatgtt
    17041 ggaagtgttt gtggagctgt aaatgctgct gttgttataa tagggtattt aaatgggcga
    17101 aatagtaatt tagatgaaaa tatagcaagg aagtattcaa gagagctatt gaatagagta
    17161 agaaataaat acagctcaga aatatgtgta tctcttaaaa aaaataaagt aacttgtgaa
    17221 gaaataataa atttcacata tgatgctcta aatgatatgc taaaaaatca aaaataattc
    17281 taagaaacaa gtattttaca ttcgatagaa tacttgtttt tttatga
//
LOCUS       DAMJWS010000090        17178 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMJWS010000090 DAMJWS010000000
VERSION     DAMJWS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.091"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(124..648)
                     /locus_tag="PC399_003070"
     CDS             complement(124..648)
                     /locus_tag="PC399_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2593915.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(713..1465)
                     /locus_tag="PC399_003071"
     CDS             complement(713..1465)
                     /locus_tag="PC399_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2593916.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1496..2794)
                     /locus_tag="PC399_003072"
     CDS             complement(1496..2794)
                     /locus_tag="PC399_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2593917.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2811..5075)
                     /locus_tag="PC399_003073"
     CDS             complement(2811..5075)
                     /locus_tag="PC399_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2593918.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5330..6148)
                     /locus_tag="PC399_003074"
     CDS             complement(5330..6148)
                     /locus_tag="PC399_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2593919.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="PC399_003075"
     CDS             complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="PC399_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2593920.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6842..7120)
                     /locus_tag="PC399_003076"
     CDS             complement(6842..7120)
                     /locus_tag="PC399_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2593921.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7110..7862)
                     /locus_tag="PC399_003077"
     CDS             complement(7110..7862)
                     /locus_tag="PC399_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2593922.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8044..8236)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8368..10140)
                     /locus_tag="PC399_003078"
     CDS             complement(8368..10140)
                     /locus_tag="PC399_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2593923.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10154..11083)
                     /locus_tag="PC399_003079"
     CDS             complement(10154..11083)
                     /locus_tag="PC399_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2593924.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11312..11500
                     /locus_tag="PC399_003080"
     CDS             11312..11500
                     /locus_tag="PC399_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2593925.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11493..12194
                     /locus_tag="PC399_003081"
     CDS             11493..12194
                     /locus_tag="PC399_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593926.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12196..13119
                     /locus_tag="PC399_003082"
     CDS             12196..13119
                     /locus_tag="PC399_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2593927.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13276..14199
                     /locus_tag="PC399_003083"
     CDS             13276..14199
                     /locus_tag="PC399_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593928.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14192..14929
                     /locus_tag="PC399_003084"
     CDS             14192..14929
                     /locus_tag="PC399_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593929.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14940..15713
                     /locus_tag="PC399_003085"
     CDS             14940..15713
                     /locus_tag="PC399_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593930.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(16254..16862)
                     /locus_tag="PC399_003086"
     CDS             complement(16254..16862)
                     /locus_tag="PC399_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2593931.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17040..17156)
                     /gene="rrf"
                     /locus_tag="PC399_003087"
     rRNA            complement(17040..17156)
                     /gene="rrf"
                     /locus_tag="PC399_003087"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5530 a         2987 c         2172 g         6489 t
ORIGIN      
        1 aaataaatta aaaagtgaaa tgtatttgtc taaaaacata ataacatttc actttatcct
       61 catttttaag taatatatat ttttaatttt tatttataac taaatctagt ttttatataa
      121 attttagttt ttttctacat ctgagtaatc tattccaaaa gtttctacag taacagattt
      181 cataatttgt ggagtttgtg gcttatcagc tgaatctgtt ctaactgatg ctatttcatc
      241 aactgtatcc ataccttcta tcactctacc aaaacctgca tactgaccat ctaagtgtgg
      301 tgagttttta tgcattatga aaaattgaga accagctgaa ttaggtgcca tagttctagc
      361 cattgatatg actcctcttt catgttttaa attgttagta aatccattac cactaaattc
      421 accttttatt gagtgacctg gaccacccat tcctgtacca tttgggcatc ctccttgtat
      481 cataaatcct ggtattactc tatgaaatat tattccattg taatatcctt catttactaa
      541 tgatacaaag tttttaactg tatttggagc tatatttgga tataactcaa ttttgatttc
      601 ttttccattt tccatttcta tagttactat aggattttta ttttccataa tttattacct
      661 tccttttcat tagttaaatt acataattac atttattata ttatactcta aattaaagtc
      721 tatcaagtat attttttcct atttcatatc cttcttgata aatagtttta caaaactcat
      781 catcaaagtt aagcgtgtct ttaggagcaa attgagtatt aggtctaact actattattt
      841 tatcttcatc atatactttt ttaatatctt ctggcaactc ataattatat tttgtggata
      901 ttaaaataat tttatccaca ttatccccaa ttaatggttc aattaatgca ctcctaatta
      961 aacctccatc taatttgaaa ccatcataaa ttccttcttg cacatctttc acaaattgtt
     1021 ctttaaatcc aagagttgcg tttggatttg atggtaacaa cccactgtat gaaatgctga
     1081 taatggcttc attacgaggc atttttgata catcaacaat cttttctgat actactttat
     1141 tatcaacttc aacataatta acataaaagt tcatttcttc tatattacta tcatctagtt
     1201 cccttaggtt gtctatagca ggagaattat ccacagtatt atttactatt tttacatttc
     1261 cattctggat atcttttata tttgtccaca ttttctccaa gttatcaaca ttccctgtgt
     1321 acaaataata tccatttatt gctcctattg atgtccctga aatcgaataa aatttattta
     1381 ttcctccatc atacaaggct ttaactactc ctgcttgata agcccctttt gctcctccac
     1441 cttctaaaca taatcctaat ttcatagtct catctccaaa ttgtttatct tatatttata
     1501 tttcaaaaaa cttactaaat gttttatata tactatagtg gtcctcaaca tttcctaatt
     1561 ctcttataga atgcattgct aaaattggac ttcccacatc cacagatggt atatctatat
     1621 gagtagaaga tattggacct atagtacttc ctcctctttc atctgaccta ttaacaaatt
     1681 cttgatactc aacattagct tctctacata tatttttata aatagctata gaatatccat
     1741 cactagtata cgcctgacta gcatttatct ttataaccgg accttttccc attattggtt
     1801 tattagttgg gtcatgcttt tctggaatgt ttggatgaat agcatgagct aaatctgatg
     1861 atatcataaa agatgaataa atagactcaa agaatccctc tctatcctta cccattgata
     1921 tacaaattct ctccaatata tttaacaaca tgtttgaatc tgcaccttgt ttagtagagc
     1981 taccaacttc ttcattgtca aatacagcca ttacatttac accatgttga cctttagtat
     2041 taacaagagc attcaaacta gcatgtacca ttgcaagatt atcttgtctc ccaatagata
     2101 taaattcatc attagcccct attaaacttc ctttttcaaa ttcatataaa aataaatcaa
     2161 aatctataat atcgtccata tttacatcta agttagatgc tatttgcttt ataagaaaat
     2221 tatctttttc taatgtatca tttaacatcc caactaaagg taacatatct ttttgcttat
     2281 tatatgaatg accatcattt atacttctat ttaaatgtat tgctagattt ggtattatac
     2341 aaattggctt tttaaagtct ataagccttt catttggttt taatatacta tcacctttta
     2401 aaactactct accagctatc ccaagaggtc tatccaacca tgtacttaat attgctcctc
     2461 catagcattc tgtatttaac ttcaaataag atttttcagc ttctatttct gcatttggtt
     2521 taatactaaa tgtaggagaa tctgaatgag agcctattat tctaaatcca tctctttcta
     2581 tatcttctga atttactaca aaagctacta tagctgataa attcttagta acataatact
     2641 ttccaccaac tcttagattc catctttgat tcaaaactaa ttcttcaaat ccatttttat
     2701 ctaagatttc cttagtactc ttgactgcat gaaaagacga cggactatca tatatgaagt
     2761 ctattaagtt tttagcaaag tttatactat ccattcctca tttacctcca ttataaaata
     2821 tttagtgttg tatattcttt atttattttt ttaattccct gttggtcaaa agctatagta
     2881 acatctgtac caattataga aaccactgtc ccaaccccaa atttaggatg atgtacctta
     2941 gaaccaagtt ttatatcatc tggattagtt tctttattag aatcttttat tgtagcatta
     3001 actttatctg caactttaga tttgcttata gtactcatat actttttagt atatttatct
     3061 aacatattat aacttgcttt agaatagctt agttcttttt ttactttgta caatctttct
     3121 atacattctt ctggcagttc ttccataaat cttgaagcta ttgcaacatt tgttttacca
     3181 tataatgttc tcttctctgt caatgacata tatagctctt ctttagctct agttatacca
     3241 acataacaaa gtcttctctc ttcttctatt tgtgaatcac tcatagattt tatagctctt
     3301 gatataggaa ataacccttc ttccatacct gttaaaaaca caacagggaa ctctaaacct
     3361 ttagatgtat gaattgtcat aagcgaaacc ctatcattgt cttcttcatc atttgattca
     3421 gatgtaagtg ctatacttgt taggaatgtc tctaagtctt tattttcata agtatcatca
     3481 ttactacttt caaactccaa agctatagaa ataaattctc tcaaattatc aattctgtct
     3541 tgagcttctt cacctttgcc tgttaaatct ttttcatttt tatttcttat ctctacaagc
     3601 tcgtccatat atcctgtagt gtccaagact ttttctataa gtttgctaac aggataaact
     3661 tcttttatag ttctaagagt ccctataata tcaacaaatc cacttatact tgcccttgcc
     3721 ttagtagata tatctgaatt tgtttctata tcaattaata ctgagtatat actttcctgt
     3781 tttaagttag ctctatcttc aattttttct atggttctaa gccctatacc tcttctagga
     3841 acatttataa tcctttttat agatatatca tcttgtggat tttgaataac tcttaaatac
     3901 gccaacaaat cctttatttc ttttctctca tagaattttg taccaccata tatattgtat
     3961 ggaatttgac ttctatttaa agcgtcctct acaggtcttg cctgtgcatt tgctctataa
     4021 agtactgcaa aatccttata aggtctattt tcttttctag ctatttgtgc aattgaatca
     4081 gcaatgaaat cagcctcttc tatttcacta ccagttaatt gtatctttat aagttctcct
     4141 tctttttttt cactccaaag cttttttctt tttctttcta tattattaga tataacttta
     4201 ttagctgcat ctaatatcac ttgtgtagac ctataatttt gttccaactt tacaacatgt
     4261 acattgtcat aatctttctc aaattcaagt atgtttctta tatctgcacc tctccatcca
     4321 taaatacttt ggtcatcatc tccaacaaca caaatatttt gatgttctct tgctaataat
     4381 tttatcaatt catattgagc tttacttgta tcttggtact catctaccat tatgtatcta
     4441 aatctacttc tgtaatatgc taacacttca tcattagcct tgaaaagttc tacagtttta
     4501 agtattaaat catcaaagtc aagtgcacta tttcttttta aacggtcttg atatagcgca
     4561 taaatatctg caatcttaac cattctatta tctgccatat tgatatcttt aaactgcttt
     4621 ggagtataaa gtttatcttt tgctcctgaa attgcactta taactgcttt tggttcaaac
     4681 actttatcac ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac ttggtctgct
     4741 gaatcataaa ttacaaaact tctattgtaa ccaatcttat ttatatcttt tcttagtata
     4801 cgaacacagc aagaatggaa tgtacttatc cacatatcct tagtttcagg acctatattt
     4861 tgttcaactc tctctctcat ttcatttgca gctttgtttg taaatgttat agctaatata
     4921 tttggtgctt gtacaccttt atcttctata agatatgcta ttcttgttgt taatacctta
     4981 gttttacctg aaccagcacc agccagtata agtactggac cttcagtctt ttctactgct
     5041 tctctttgag caggatttaa tgtatctaaa ttcatatttc tcctcctgtt aacagccata
     5101 aatatatttt aatctaaaat atgaattact tcaaattata attatgtatt atattaatag
     5161 attatttcca atatattaca attaaataac atttttccat ataatttatt tcattgttta
     5221 ataaaaaaaa taaccttatg atttggggaa tcataaggca cctttaaggg gtctaggtta
     5281 agggttatta tatataaatc tattttttta aaataggttt agggcttaat tatttatttg
     5341 tattaagttc tctttccttc caatacttat acaaatcttc ttctttctta aataagggta
     5401 aattcatatt tctatgtact tttactaacc atggggattc taggccagct tgtataatat
     5461 tattttcatt tataaacaca ttttctgctt ttccttcatc aatcaaaatc cccttatcca
     5521 atacatatat gtaatcacat atttcataca ttaaattcat atcatgactg gatattacta
     5581 tttttatatt atttttattt aatcccttta ttatatctac aatagaccta gtacttactg
     5641 ggtctaaacc agctgtaggt tcatcaagta atacaatttc attttccatt gctataactg
     5701 atgctattgc cactcttttt ttctgtccat agctaagaaa atgtactggc ctgtctataa
     5761 aatctattcc attaacagct actagagctt tgtttattct ctctttaatt gttttttcat
     5821 ccataccaat gtttctcatt gcaaaggcta tatcatcata tacttttgaa taaaaaattt
     5881 gtttttctgg gtcttggaac acaataccta cattttttct aaggttgtat aaacttctct
     5941 tatcatattt taacttttct tctttaaaaa gaatttctcc agatgtaggc tttaatatac
     6001 ccattaaatt cataaataat gttgattttc ctgaaccatt tgaacctatt atacctataa
     6061 catttccctt tgaaaaatcc atattaatgt ttaatagtgc attggtattt ttttcatatt
     6121 gatacgttaa attatttatt ttaaacatca ttatccccta ctatgtgaaa tttaccatca
     6181 tacaatttta tgtctaatga tatacacata tcatcatacc ttgtcataag tcttttgtac
     6241 agcatacttc caagaatacc taaggattta tatgaagttt ttaaatttat atatccaaat
     6301 cttagctctt gtgacttgta tatttctgaa aattcttcta aaaatatgaa tataaaccta
     6361 tatattaaca ttgataactc gacaaaagtg tcaggtaaat gaagtttttt aaaaaagaat
     6421 attaattgat taaaaggtgt tgtaagtata caaaaataca cacaagtcaa acaagacatc
     6481 gctctaaata acacatgaat agacatgtca atagacgcct ttgatattcc tacattaaaa
     6541 ctaaaaaatt caaaactata taataaatct gctttattaa aagatatatt tacaagagtt
     6601 attcctatac taagaaataa aaagtacatt ggtattctaa gtaattttaa ataatttttt
     6661 aaatctattc cagccacaca aacaaccaaa atactcatta aaatcataat tccacttaaa
     6721 ataaatatat tttgtattac catagaagca atcagaaaaa ttactcctat ggctactttt
     6781 ttgtttggat ttactttact caatctattt gtatatgcat atttatcaat tataagcatg
     6841 attaacttct ctttcctttt tgaagaccta atatgtatcc tattacacca gcccctaaag
     6901 ctgcttgtgc agagaataat aaactttcta tttctccact tggtggctca tatggactgc
     6961 taaaccatgg tttgtaattt ggatttatct tagtaatttc actttcagct tgaccatctg
     7021 ctcctccata ttcagcacct gagttgacaa gtaatgggaa tataataaga gctactacta
     7081 ataaaagtaa taatatgtta gtttttgttt tagcactcat tagctttaac tcctttatca
     7141 ttattttcta gtatgctttt ttctccgttt tctgtaatta aattatatac aattacagta
     7201 agtatacctt ctgctattgc tattggtatt tgagtcataa agaaaattcc taaaaattta
     7261 attgctgaag ccattacccc acctgatggg tctgggaaag ccagtgctaa ttgtattgaa
     7321 gttattgtat atgtggctaa gtctcctatt gctgctgcaa gaaatactgg aatagcatta
     7381 tttccatctt ttttctttaa agccttaaat attaggaatg atacgattgg ccctattatc
     7441 gccattgaaa aagcattagc tcctaaagta gttattcctc catgtgctaa taacaatgct
     7501 tggaatatta aaactatagt tccaagaaca aacattacgc ttggtccaaa cattattgct
     7561 ccaagaccta caccagtagg atgtgaacaa cttcctgtta ctgaaggtat ttttagtgct
     7621 gataatacaa acacaaatgc tccacataaa gctaataata ctttcttttt agggtcttgc
     7681 tttactattt tacctattga ttttaatccg aataagaaaa atggtataaa tataactccc
     7741 catgctatag accattttac tggtaagtaa ccttccatta tatgcattgc atttgatgta
     7801 actggagtta aaatgattaa cataagtgca gctataaccc ctaatttaat attctgtttc
     7861 atcttagtat ccctctctcc ctttctaata attaaaacat aataatacat gtctgattta
     7921 tttaataaaa taaaaaagcc taactcgatg gttaggcttc taattttcca caatctaaat
     7981 acaaacacat aaaacaaaca tatctgtata attattataa gaaactttct ccatccccga
     8041 agaaattact ttaatatttt aggcaggtct cctgacttag cttcatccta ctcctcttcc
     8101 ttcccatgag atataaaaaa tatcatcata gtggctaaca aaagtttcgt ctgcatcaca
     8161 gtagcggggg ctgtaaagga atttaacctt tttccctatt aatcttgctt aaagaaccta
     8221 taatattata tatttaattt ttaaattctt cttaaattat tttctatata aatcattgta
     8281 taccttttct gtgaaaattt ctacattttt tataaatttt attcattttt atatttttat
     8341 gtatataaat tcttttttgt aagtttatta atttctaatt atcttcttta aatacattga
     8401 aactacatca gaaaatctag ttatatctct tgaatatata aaatcatttc catatattaa
     8461 tctttcagct tcaagttctg attcttttcc tgtaaatact cctaatacta atatattttg
     8521 ctttctagct tttctcactt cattggctgt atctctcaaa ccaactatac ccctataagc
     8581 cttttctcct cttaatgttc tctcactact ttttcctatc tttacatcat ttggtcttcc
     8641 atcactgaga actatcagga ttttattttc ttcatctcta tttattagac cactacatat
     8701 agattttatt gccaaaccat ctctattatt accagcacaa aaatattcaa atatattttc
     8761 actatttttt atactatcat cataatcttt aaatcttttt aatattgtat agtctaaaaa
     8821 actagaaaat cccatcactc tatttggtat gccaacttct gttagagcat ttgcaattat
     8881 gtaagcttga attgccacat ttccctgatt tctactttga gagccacttg catccaataa
     8941 tatatcaata acgtatttcc ctttttcatt ttctatatct ttataaaata ttttattatt
     9001 attacttcta ccaactctcc aagctctatt ggctaatata gtaccacaat cagaatatac
     9061 tctactttta gcattttctt ctattaaaat tcttgataga ctttctttta atctctttat
     9121 ttgctgtcta tacattctag catgttctct aaattttcct aaattatttt ctttttgtct
     9181 tgttacatat tttagttgaa atacattatt acactcactt ctaagaactc catctgtaaa
     9241 gtgtattcta caaccttcat gaacatttct acaattttgt gtctcaattt ttttaatttc
     9301 acctttactc aaataagttt ttccataata atgttctatt tttgaatata tcttatttgc
     9361 agtgtcttca tcaacatata taactcggtc tgctgaattg ctacatttag aatcatcaaa
     9421 atcccctaca ccttctacaa gcatggatga agatattgaa tctatatcac tttcaataat
     9481 attttcttct tcatcactat aaagttcttc atacatgaaa tcagcaaatg tatcaaaatt
     9541 tacttctaca ctttctacat tattttcatc aataagacta ttttcttctc catttaatat
     9601 gtattcaaaa taagttaaat atataacatc taatgctttt aaaatctcca taatatcttg
     9661 attggtatct atatttctta tatcattaac tattcttcta gtcatactat ctaatgctgg
     9721 gtgcttgtcc atactataaa gtacaaatgt gtatttaatt ttatccataa tattatcatt
     9781 gtgtattttg gtaaaacgtg aaacaatatc tttataagca tttcttctta tgtcttctat
     9841 ccctggccta tctttaatga ccttttcttc aactattgta tttaatataa cttgaattag
     9901 acccagtata atatctttgt tgtatccttt tcttattcta tttgtaagat attttttaac
     9961 tatatcccaa tctacatact ttcttctagc acctgcaatt attgcaaaat atagacctat
    10021 ttcttttgaa gagtaatctt tttctgatat atctatactt tcattgtaat caccagctat
    10081 tgtccatgat aagttattag ctctattttc aaattcataa tcatcatata tccatttcat
    10141 aatctcacct atattaaata tgacttattg gaaacacatc tatactctcc catttattag
    10201 gtattctagt tttaattaca tctccaacca tttctttctc atatacatca aaagtctttc
    10261 ctgtaagtcc catattaatt gcaagagttg gttttaatcc tcttcttata gtcttaagag
    10321 atgccataag accacgtaaa tcaatagctt tactagatat ctctccattt tgagacttca
    10381 attgtaaatc taaaaatatt cctgcaaagt gtattagttt ttcttcatca gcatctgaaa
    10441 attcattttt taaaatcatc attaacttat cttcttctat aggaggaata tctattacca
    10501 taaatctaga taccagtgct tcattaagtt cttttgttcc tgcatattca taattcatag
    10561 tcccaataaa tcttgttgct ggatgtagat tgactctctc atacccaggt acatcaatta
    10621 ttcttctata gtctaaagct gaatgtacca caactatagc atcatttttt gccatattaa
    10681 tttcatcaaa caccccaaaa cctccattaa tagcacactc ataaactgag cctcttctaa
    10741 gtttaacttc attgtctata aatgtatctg tacctataag tgttgaactg tctgtattta
    10801 tatgaaatga tgtattccac tgaggtctat taaatatttc ccctagatta tcagcaagta
    10861 agttttttcc cgttgccttt ggtcccgata ataatatatt ttcttcttct aaaatagctg
    10921 ctatacacat agacaagata tctttaccat aaaaataagt tttagacttt gttactctgt
    10981 attctaaatc tttttctaat ccataataat ttctaaaata cagtacatct tcaataagtt
    11041 tttcatctac cccttgcacc cttagattat ctattatatc catattttta ccccttttat
    11101 ataaatagta tttgtttcat ttcttatcac cattttagca taaaaggata taagctaaaa
    11161 ttgatttttt aatctccccc attttaagta aaaccatgca ccattttatc actacaaaat
    11221 taagagtact acttactgat atttcttgat tttattcgtc tatacgtata taatatatac
    11281 tgataaatta tgaggaaagg atgattgaaa tatggaatat atgtcttgcc cagaagtagc
    11341 aaaaaaatgg ggtatttctg aaagaagagt acaaaaacta tgtgaagaaa gtcgcatacc
    11401 aggagtttca aaactcgggt atatgtggct gataccaaag aacgcagaaa agcctgttga
    11461 tggtagaaca aaaagaagaa aggaagtaca atatgaatag cgttttgatt atagatgatg
    11521 acaaagaact ttgtgctctt atgaaaaaat gtgttgagca agaaaattta tctgctatta
    11581 ctgcacatgg tggtgcagaa ggtctacatt tagtcgataa aaatagacac acactctctc
    11641 ttgtaatttt ggacataatg atgccagata tagacggttt tcaagttctg aaaaaaatta
    11701 gagaaacaag taatattcca gtaattatgc ttactgctaa aaatgacgag gatgataaag
    11761 tttccggact acgtttaggt gctgacgatt acctaacaaa accgttcagc ataaacgaac
    11821 ttatggctcg tgtcaattct cttattcgtc ggtatactat gctaaaccct gcttttataa
    11881 cagacacaga tttcataata ataaatggca tgattattga caagacaaac cgaattgttt
    11941 ctgtcaataa tattcctgtt caacttacaa gcaaagaatt tgatttactt gcttttctgg
    12001 cttctaataa gggacgggtg tttaccaaaa aacagattta tacacaagta tgggaagaag
    12061 aatatgcttt tgacgatagt aacatcatgt ctttcatcag taaattgaga aaaaagattg
    12121 aaccagaccc caaccatcca ttttatattt tgacagttcg tggtgtcggt tatcgtttta
    12181 ataaggaggc ttaacatgaa tacaagtgtc tatctgcttt tgatattggc tcttagcctg
    12241 attattatag cgtaccttgt ttctgtcctg ctacgtgtcc gaatacaact tacacttata
    12301 aaagatgctt tagaggatat aaaaaatgga aaccttaatc gacgtatttt aacagatgaa
    12361 aacgacatta caaaacaaat ttgctatgat attaacgaaa tagcaatcaa tagccaattt
    12421 caacttattc agcaaaaaca atcagaacaa gcctacaaac agctaatgac aagtctctcc
    12481 catgatgtaa aaactccact tgcttctttg gtcggttatt tggaagcagt agaaagtaaa
    12541 attgttgtag gagaggaaaa agacgagtat atccatgttg cgtctaataa ggctcattac
    12601 ttaaagaatt ttgtagaaaa tctatttgaa tgggtaaaat tggattctaa ggaacaagtt
    12661 tttcattttg atatttttga cttgaatgaa ttatcccgta atattatatc tgattggata
    12721 cctgttttag aaagcagtca ttttgaatat gaatttgata taccagaaat agagtacttc
    12781 ttacgtatag acgcaaatgc atatacccgt atcattaata acctattaca aaatattatt
    12841 acccatagta gtggaaacaa aatgacattg agaatttttg aaaacaaaga gcaagctcaa
    12901 attataataa cagataatgg gaaaggtata tcttcagaca atctcccaca tatttttgaa
    12961 aggttgtacc aatgcgacca ttcacgagct tcaaagggaa atggtttagg cttggcgatt
    13021 gcaaaggaac ttattaacgc tcataaggga aatattaccg ccaacagcac tcccggtatg
    13081 ggaacagaat ttacaatatt gcttcctaaa gccctgtaaa agtacgaggc tttcttactc
    13141 taaaacaaga taaaagcaag attatggcaa ggttttggca aggttcatgt tttataattc
    13201 taattgtagt tgattttgac gttgtatcaa ctatcttttg tagaaacaaa tttattaaaa
    13261 gtgaggaaat tatatatgaa tgattttgtg attgaaacaa agcaacttac aaaaatttat
    13321 gggaatcaaa cagtagttga caaagtcaat ctccatgtaa aaaaaggccg aatatatggt
    13381 ctgttaggac gcaacggagc tgggaaaacc acaattatga aaatgatttt aggattgact
    13441 tcaatcacgt atggcgaggt agatgtattt ggagagaata ttaagggacg tgaaaaacaa
    13501 gtatatcccc gtatcggtgc aattatcgaa acgccaggat tttatcctaa tctgacagga
    13561 acggagaact tggagatttt tgcaaaactg cgtggtacag ccgcccctaa ttctgtaaaa
    13621 agagcattgg aaattgtagg cttaccttat aaaaataaaa agctgtttag taaatattca
    13681 cttggcatga aacagcgact tggtattgcc aacgctatcc tgcatgaccc agaactgtta
    13741 gttttagatg agccaaccaa tggtcttgac cctattggta ttgcggagat gagaaagttt
    13801 attagggact tgagcgttga gcatggaaag acaattctta tttccagtca tattctttca
    13861 gaaatctcat tgttggctga tgatattgga attatcgacc acggtgttct tttggaagaa
    13921 agtagtatga gtgaactaga aaagaaaaac agtaaatata tcctgctaca agtttcagat
    13981 attccgaaga catcacttat tttagagcgt caatttaaca cgaaggatta ttccgttcaa
    14041 gatgactaca cgttacacct ttataacact aatctggata tgggtgaaat caataaagca
    14101 cttgtaatgc agaatgtaac tgttattagt tctcaacttt gtaatgatac tttagaggac
    14161 tactttaaaa aaatcacagg aggagagggc attgcttaaa cttattcaaa cagaatttct
    14221 taaactgcgt cgtaggaaac ttatttggct gatgttgttg tctgctctca ttatgccgtt
    14281 tttcgcacta ctatacttca attattttgg taaaacaggt gttgagccaa taaagtttta
    14341 taaatggtct gcatttggtt atacactttg gattattttg cctgttgttt tgggtatatt
    14401 ttgcaccatg cttatacacg aagaaaacca gtatgatatg ctcaaacagc tttggattgt
    14461 acctattagt aaaatgggat acttttttag taaatttttc gttgttttga tttactctat
    14521 ttgttttatg ctgattacag ccgttgcttc tgtgttattt agtgtacttt ctggatatgt
    14581 cgtatttagc tgggcaagca ttttatattt gttgaaaaaa tgtttagaaa ttggtgtaat
    14641 cactgctttt gtaatgttgc cgattttggc aatcgctaca tctcaaaaag gatacattct
    14701 cccaatttgt atcacattgg tatatgcctt ttccggtttt gttataatga caataaatat
    14761 gtatttacac ccattatcca gtatgggagt tattatcatg agaaataaag acattccagg
    14821 tcttatattt tcacaggaaa ttaatgtccc attagcattt ctttgtattt gtatttggag
    14881 tattgtatca gtattgtttg caaatattgc actaggaaga agaaagtgag gtgtagataa
    14941 tgtggaaatt actttgggct gaatgtcaaa aaattcgtcg ttcaaaaatt gtttggatta
    15001 cagtttttgc atccattatg atagcagtca tagtttttgc aggggggcag gatgtatata
    15061 atgacccaga tttacattat ggcttaaaga gcgtttatga cggttcacgg tatatagaca
    15121 atgcaggttg gtatatggac gcagtacaac cttggtcaac tttttttgtt cttcctgctg
    15181 tcattgccct attaggaagc tatattattt gtcgtgaaga agaggaggac actattaaat
    15241 ccctgcgtct tattcccata aatgaaacaa atttaaccat agcaaaaatg attattactt
    15301 ttatattcag tatacttctt tatttgttac tctttactat aacatttttg actgaatccg
    15361 ttttacattt ttccgattta tcgacaaaat tggttttaag ttgcatgaaa gagtattttc
    15421 ttgatggcat aggtgtattc tttgcaattt ctcccattat tgccttggta tcgcgcatga
    15481 aaaaaggata ttggcttgca ttagtattca cagaagttta ttctattgct ggactatttg
    15541 cgggtatgtc aaatgtctta caaacctttt ttcctattgc ggcaatattt aatatttctg
    15601 gatactatat aactactgga gaaaaaatta tggggagtat catgagttta ttactttgtg
    15661 cctgcctttc tgcatttata cttaaagggc taaaacacaa tgaaaaaaat tgaaataggt
    15721 atatcaaaat gaaaaagata aaaaaactga ttattctaat tgtattcttg tttctttttg
    15781 gcatgttaca ctgttatact gaatggaaat tcaacaataa cccacctaaa ggggtaatag
    15841 aaagaataaa tgagtggact gataagattg gactggatta gtaacttatg ttaattatag
    15901 tgaatgtaat attagcaagt tattgtttcg tattttaata ctataacttt aaatatttgg
    15961 ttttaatctt ccaaacgaat ataaaaaaac tgtcacagag gcaacatatt tttgaatatt
    16021 tataaagtgg tagttttgat tttcaaagct accgcctttt cttctatata agcaaactta
    16081 tcctcaacaa agtgtaaaac atggtgcatt ttaagaaaaa agtaccacag acacaagcaa
    16141 taaaagaaac gccgagaacc tacaaaaacg tagtgcttat gcagatttac gacgccttct
    16201 attttctcaa ccgtcttaat gaccttgtac aaaagaaatt tttctaatat tttttataat
    16261 cttatcttgt gatgtttttt atgtataaat gacttaataa caaatccaat aaaaattatg
    16321 gatattggta taattagaac gaggaattta taatcattat aatgtgacat tattcttacc
    16381 caactctcag aaaaaatata tcctatactg attaatgaag tattccatat ggttattccc
    16441 acgatagatg atattacaaa agttataata ttttgacctg ctatacctgc tataaaagat
    16501 atgtatgttc tacataaagg gattaatcta catacacaaa cagaaataga agagtattta
    16561 ttaaaataat cttgagatgc aaatattcct ttctttgcct ttggacatat ctctattatt
    16621 ttatttatga tagatttacc tccccaattt cccactgtat aacaaaatat cgaaccaagt
    16681 atacctgcaa ttatacttaa aattaaaatt gtcagaaatc caaaatgatt tcttgcagca
    16741 attgctccac ataaaggtaa aacaacttcg ctaggtaatg gaaaacaagc atactcaatc
    16801 ataattaata caaatacaga gattaatcca tattgtaaaa taaaactatt gattacagac
    16861 acttacttat ctatctcctt tcttatttaa aggttttaag ggtacatgtt ctaaggattc
    16921 tcaccctata tttaaagaat caatctcttt aaactattat atgtatttac acaaagcatt
    16981 tataacatac tttaaaaata gtaccttaaa ataagaaact taataaataa aaaaagatta
    17041 cgtggctacg tcctactctc ccaggcagtt tcccaccaag taccatcagc gctagagagc
    17101 ttaacttctg tgttcggaat gggaacaggt gtatcctctc tgctattgta accacataat
    17161 ctttatgctt aatatttc
//
LOCUS       DAMJWS010000091        16888 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMJWS010000091 DAMJWS010000000
VERSION     DAMJWS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.093"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            283..741
                     /locus_tag="PC399_003088"
     CDS             283..741
                     /locus_tag="PC399_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2593932.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            802..1077
                     /locus_tag="PC399_003089"
     CDS             802..1077
                     /locus_tag="PC399_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2593933.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1136..2410
                     /locus_tag="PC399_003090"
     CDS             1136..2410
                     /locus_tag="PC399_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2593934.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2540..3592
                     /locus_tag="PC399_003091"
     CDS             2540..3592
                     /locus_tag="PC399_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2593935.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3592..4623
                     /locus_tag="PC399_003092"
     CDS             3592..4623
                     /locus_tag="PC399_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF2593936.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4715..5527
                     /locus_tag="PC399_003093"
     CDS             4715..5527
                     /locus_tag="PC399_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2593937.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5530..6450
                     /locus_tag="PC399_003094"
     CDS             5530..6450
                     /locus_tag="PC399_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2593938.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6625..7071
                     /gene="rpiB"
                     /locus_tag="PC399_003095"
     CDS             6625..7071
                     /gene="rpiB"
                     /locus_tag="PC399_003095"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2593939.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7101..7766
                     /locus_tag="PC399_003096"
     CDS             7101..7766
                     /locus_tag="PC399_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2593940.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7876..9378
                     /locus_tag="PC399_003097"
     CDS             7876..9378
                     /locus_tag="PC399_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF2593941.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRTYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9690..11279)
                     /locus_tag="PC399_003098"
     CDS             complement(9690..11279)
                     /locus_tag="PC399_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2593942.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSNI
                     NYYYREFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVKF
                     LENVINLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIYE
                     NIKYNNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFM
                     YIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVI
                     GDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDML
                     KNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQS
                     IVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDM
                     FIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENA
                     HMEIIIDADLFKVMLTFRKVN"
     gene            complement(11248..11964)
                     /locus_tag="PC399_003099"
     CDS             complement(11248..11964)
                     /locus_tag="PC399_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2593943.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12503..13303)
                     /locus_tag="PC399_003100"
     CDS             complement(12503..13303)
                     /locus_tag="PC399_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2593944.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(13617..14348)
                     /locus_tag="PC399_003101"
     CDS             complement(13617..14348)
                     /locus_tag="PC399_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593945.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(14335..15030)
                     /locus_tag="PC399_003102"
     CDS             complement(14335..15030)
                     /locus_tag="PC399_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2593946.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(15032..15823)
                     /locus_tag="PC399_003103"
     CDS             complement(15032..15823)
                     /locus_tag="PC399_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593947.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            complement(15833..16645)
                     /gene="modA"
                     /locus_tag="PC399_003104"
     CDS             complement(15833..16645)
                     /gene="modA"
                     /locus_tag="PC399_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2593948.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         6431 a         2068 c         2711 g         5678 t
ORIGIN      
        1 aaaaaccatt tgtaaaaaac aaaaaaatca tttctctaac aaaaaaaacc acaaaaaata
       61 taaaaaagta tggctagtta agtgattaac aagtaaaaat taaaataaaa atcaaaaaaa
      121 atttatcaat gaaagtcagc taaaatcctg gaagagagca ttatttttgg gaaaacattt
      181 tcttttgttt cacaaaaaaa gccacttttt ggcacacaac atgctttata taaagtcaag
      241 aaaaactaag gtataagaca aaggcaatta ggaggtgtaa atatggagtt tataaatttt
      301 gtaagtgata aagttataga ccttaaaatg gattgtaaaa cgaaagaaga tttctttaaa
      361 gaaatacata ataaagtttt tgatttaggc tttgtaaaag aagaatttgg agaaaaaata
      421 ttagcaagag aaaatgtatt tccaacaggt ttaaatttag gagattatgg agtagcaata
      481 ccacatacag atgcagaata tattaaagag caatttatat cagtctgtac ttttaatgaa
      541 ccagtagtgt ttagttctat ggaagaccaa gatgaaaaag tacctgtaaa tttggctttc
      601 gtattaggac ttaatcaacc acacagtcaa ttatctgtat taacagaatt aatgggaatt
      661 atgcaaaata aagagttagt tgataagttg atgagttcta ctgataaaga agaagtttta
      721 aagacactaa agtctttata aaaataaaaa agtactaaaa ataaaaaata taaaaataaa
      781 aattattagg aggaaataaa aatgaaaaaa atattagtag catgtggagc aggaatagca
      841 acatcaacag tagtttgtga caaggtagag agattagtta aggaaaataa tatacaagca
      901 gaagttatac aatgcaaaat agctgaatgt gcaacaaagc aagaaggagc agatttaata
      961 gtatcaacaa ctatactacc aacaacttat aatataccag caataaaagc aacagcatat
     1021 ataactggag taaatacagc agcattagat aaaaaaatat tagatgcttt aaaataatcc
     1081 tatcatagat acattgaaat aatattttat aaaacagaaa aaggaggatt tataaatgga
     1141 taaattatta gcagtagtac aatacatttt aaatatggga ccgacagtaa tactaccaat
     1201 aacaattgta gtactaggaa tgatatttag aattaagttt acaaaagctt taaagtcagg
     1261 tcttacaatt ggtataggat ttgtgggtat aaacttagtt gtaaacctat taacatcaac
     1321 actagggcca gcggctcaag gaatggttga aagattcgga cttaatttaa cagtaataga
     1381 tgttggttgg ccaacaacag catcagcaac atgggcttca ccagttgcac caatattaat
     1441 accagtttgt ttattagtaa acttactatt attatacttc aataaaacaa agatattaaa
     1501 cgttgatata tggaattatt ggcactttat agtagcaggt gcagttggat atatagtaac
     1561 aggaagttat gtttgggcta tattctgtgc aatactaatg gagttattag tactgtttat
     1621 tgcagaaaag acagcaaaaa atgttcagga gttttatgat ttagaaggta tagcattacc
     1681 aactggttca acagcttcat ttgctccact tggatattta gtaggtaaat ttgttgaaaa
     1741 aataccagga ataaataaaa tacaagcaga cccagactct atacaaaaaa gatttggtat
     1801 atttggagaa ccaatgatga tggggattat actaggtata atacttggag cacttgcggg
     1861 atatgatgta gcaggaatat tcaatgtagg tatatcaatg ggtgcagtaa tgttacttat
     1921 gcctagaatg gttagaatat taatggaagg tttaattcca atttcagaat caataagaga
     1981 tttcttacaa gcaaaatatg gagacagaga actttatata ggtctggatg cagcagtagc
     2041 aactggacat ccagcagtta tatcaacagc tcttatactt gttcctataa ctttgttctt
     2101 agcagttata ctaccaggaa acaaagtttt accatttgga gacttagcga caataccatt
     2161 ttatatggca tttatagtgt gttttagaaa aggtaatata gttcaatcag ttataacagg
     2221 aacaatagta atagctatat cactttatat ggctacaaac tttgcagatg ttcatacaca
     2281 attattacaa caagcaaata tgatgccaga aggaacaaca atggttacaa gtatagatac
     2341 aggtggaaac ttgctaaaat ggggaatatt gaaattatct caattagttt cttcatttgg
     2401 aatattctag tatgtagtta atagagatta ctctttttta gtaaagtatg cgaaaattta
     2461 acatttagca gctactaaaa aagagtaata ttttttataa atttttaaaa atacaaaata
     2521 caagaggagg agatttatcg tgaaagcagc agtattacat ggcacaaatg atatgagatt
     2581 tgaagatata gaaataaaac catgtgaaag tgatgaagta aaaataaaag ttatggcagc
     2641 aggaatatgt gggtcagatc ctccaagagt attaaaacat tggaaatatc cagttccagc
     2701 tataccagga catgagtttt ctggagtaat tgctgaagtt ggtaaggatg taaaaaatgt
     2761 aaaagttgga gatagagtag ttgcaattcc ttttatacct tgtaatgaat gtgaatattg
     2821 taaaagagga ttattttcat tgtgtgatga ccatggaatg ttaggagcta aaagttttgg
     2881 agcttttgca gagtatgtaa atataaaagc tacaaatgta cttccaatag gagatatgaa
     2941 ctttgaagat gctgcaatga tagagccact agcagttgct atgcatggag tacttaatat
     3001 aggtgttcaa gttggagaca ctgtagctgt aatgggtagt ggaacaatgg ggcagttggt
     3061 aatacaagga cttaaaatag caggggcagg aacaattata gcagttgata tatctgataa
     3121 taaattaaga gaatcaaagg aattaggggc tgatataata attaatgcta aagatattaa
     3181 cccagtagaa aaaataaaag aacttactgg tggaaaaggt gttgacatag ctcttgaatg
     3241 tgcaggttca aaaataactc aagaacaatg tttacttata actaaaaaga aaagtaagat
     3301 aggattttta gggatagcat attcagatat aactttatca gaagaagcat ttgaaaatat
     3361 atttagaaaa gaactagaat taaaaggttt ttggaactct tattctgcac catttccagg
     3421 acaagaatgg actaagggaa tcaatctagt taatgaagga aagataaaat taaaagaaat
     3481 ggtatctcat agattctcac ttgaagatac ttacaaagca tttgaaatga taagagatag
     3541 aaaagaagaa tttaacaaaa tattaatttt accacaaggg gttgaaaaat aatgaaagca
     3601 gtagttaaaa ctaaaccagg ctatgataat gtagaagttt tagaagtaga agaaccaaaa
     3661 gctacaggag acaaggttaa aataaaagta gaatatagtg gtatatgtgg ttcagatata
     3721 cattcattta aaggtgaata tgcaaatata aaagccccag taacattagg tcatgaattt
     3781 tctggaattg ttgtagaagt aggagaagat gttaaaaata taaaagtagg ggatagagtt
     3841 acatcagaga ctacttttgc aacttgtgag aaatgtatat attgtgaaac taaagattat
     3901 aacttatgcc caactagaaa aggtattggt actcaagtaa atggaagttt tgctgagtat
     3961 gtattatcaa gagaagaaag catacatgta cttcctgaaa atgtatcttt attatcagca
     4021 gcccttacag agccactagc ttgttgtgtt catgcagctc ttgaaaagac tactatagaa
     4081 agttctgata cagttttaat aataggacca ggaccaattg gtttattatt agctcaagtt
     4141 gttaaatctc aaggtgctac agttataatg tctggtgtta caaaagataa ggaaagatta
     4201 gaaatagcta aaaatcttgg agtagataga actgtaaatg taatggaaga aagtttggca
     4261 gatgtagtga atgaaatgac tgatggaaat ggagtaaaca aaggatttga ttgttctggg
     4321 tttatgccag cagttaacga agccttaaga ctgcttagaa aaaaaggtac tttcgtacaa
     4381 gttggaatct ttgctaaaaa attaaatgaa atggaccaag aagcaataat ccaaagagag
     4441 ttacaatata taggaagcag gtctcaaaag ccaagttctt ggattacagc attagattta
     4501 atggcaaatg ggaaagtaaa tacagaagct ttagttacta gaactgttgg tttagacgaa
     4561 tttaaagaag gtattacagc tttaatgaac ggggaagaaa taaaagtagc agtaaaatct
     4621 taaaaaaaca tttgtattat tttaaaaaaa attgtaaaat ctactgtagg ataaaaaaat
     4681 gtaaggtctt aaaaaataac aggaggaaga aagtatgagc actcttagag atcattctaa
     4741 taatattaga aaaaacataa taaaaatggt tgcagaagct aaaagcggac atccaggtgg
     4801 ttcattatca atagctgata tgttgacagt attatatttt gataagatga acgtatgtgc
     4861 agaaaatcca aaaatggctg atagagatag atttgttcta tcaaaaggtc atgcagcacc
     4921 agctttatat gctacattag cagaaaaagg atttttccca gaagaagaat taataacact
     4981 aagaaaattt ggttctaagt tacaaggtca cccagacatg aaaaaagtag caggaatcga
     5041 tatgtctact ggttctcttg gtcaagggct atctgcagca aatggtatgg cacttgcagg
     5101 aaaattagat aacaaagact atacagtata tactatatta ggtgatggag aaatccaaga
     5161 aggtcaaata tgggaagctg caatgacagc atcacattac aaattagata atttaattgc
     5221 atttgttgac ttaaatggat tacaaataga tggttctaat gaagaagtaa tgaatgtaag
     5281 tccagtagat gacaaattca aatcttttgg atggaatgta atagtaataa atggacattc
     5341 ttttgaagaa ataggaaatg cagttgatga agctaaaaaa gttacaggaa aaccaactgt
     5401 aataatagct aaaacagtaa aaggtaaagg tgtttcattt atggaaaata atgctgcatg
     5461 gcatggaaca gctccaaatg cagaacaagc agagcaagca ttgaaagatt tagaaggagg
     5521 tctataggaa tgggaatagc aactagagaa gcttacggac aagtgttaaa agaacttgca
     5581 gaaaataaag atatagtagt attagatgca gatttaggaa aagctacaaa aagtatatca
     5641 tttaaagaag ttgccccaga tagatttttt gacatgggga tagcagaagg tgacatgatt
     5701 ggtacagcag caggtcttgc tacttgtgga aaaatacctt ttgcgagtac ttttgcaata
     5761 tttgcagcag gaagaggcta tgagcaaata agaaactctg tagcatatcc aaatttaaat
     5821 gtaaaaatag cagctactca tgcaggtgta acagtaggtg aagatggtgg tagccatcaa
     5881 gctatagagg atatatctct aatgagaggt ataccaaata tggttgtatt aaatccagca
     5941 gatgctttag aagcaagaca agctattctt acatctattg attataatgg acctgtttat
     6001 ataagattag gtagagctgc aactcctgat gtaaacagtg aaaattataa atttgaaata
     6061 ggaaaaggaa ctgttttaag agaaggttct gacataacag taatagctac aggaataatg
     6121 gtagcaaaag ccttagaagc agcagaagaa cttgctaaag aaggtataaa tgtagaagtt
     6181 gttaatatat ctacaataaa accattagat gaagcattaa taaaagaaac tgctaagaaa
     6241 actggaaaag tagttacagc agaggagcac agtataatag gtggtttagg ttcagctgta
     6301 tgtgaagctt tagcagaaac taaagatgta gtagttagaa gaattggtgt taaagatgta
     6361 tttggtcaat caggtacacc agctgattta ttaaaacact atggtttaac tacagaagat
     6421 atagtaaaaa atataaaaga gttgttatag atataaatat atctaaataa caaattgatt
     6481 tgacataagt caattaaagc tgtcttaaaa atagaaagaa atttctacaa tgggacagct
     6541 ttatttttac aatactttat ttttatgata attataaaat agtctgaaag taatcaaaaa
     6601 aattataaaa agaggtgcta tttaatgaaa atatctattg gatgtgacca tggtggttat
     6661 gaattaaagg aatatataaa ggaaaattta ataaataaag gtatagaagt tgaagatgtg
     6721 ggtacaaatt caaaagaaag tgtagatttt ccaatatatg caaaaaaggt aagtcaaaat
     6781 gttcaaagta aaaaatctga tttaggcata ctttgttgtg gaacaggaat aggtatgtct
     6841 attgcagcaa ataagtttaa aggaatcaga gcagctgttg tttctgactg tttttcagct
     6901 aaggcaacta gagaacacaa taatacaaat gtactttgtt taggagaaag agttattggg
     6961 aaaggattag caatgagaat tgttgaagaa tggttaaatt caagttataa tggagaaaga
     7021 catgcaagaa gattacaaat gattgatgat atagaaaaag aaaatttata aatatataat
     7081 ttacaacaag ggggagcgtt atgtctatta tttgtgcttc aataatgtgt gcagaccaaa
     7141 tgaagttaaa agaggaatta gaagctcttg aagaagcagg tgtaaaatta cttcattgtg
     7201 atgttatgga tggaatattt gttaaaaact tggctatggg accagagtta ttaaaatcaa
     7261 taagtgaaaa tacaacaata cctttagaca tacacttagc cacagaaact ccagataaat
     7321 atattgatat gatgtcttat ataaagccaa aatatatttc ttttcatgta gaatcaagta
     7381 caaatgtaaa agctgatata caaaagttaa gaaattatgg tatagggcca gtgttagcaa
     7441 taagccctca aacaagtgtg gataaaatag aagaatatat aagtttagtt gagggtatct
     7501 tagtaatgac agtaaatcca gggtttgcag gtcaaaagtt taatctttct gtattggata
     7561 agttggataa acttacagag atactaaaag attatgataa tcctccattt atagaggttg
     7621 atggaaatat aaataaggat actattaaac ttatgaatgg taaaaaagta gatatatatg
     7681 ttgttggtac ttcagcttta tttaatgata agcctccaat ttcatataaa gataaaatag
     7741 aagaactaaa agaaagtata aagtaaccat gtaataaaat tataatatgt ttctgtttaa
     7801 tagtaaaaat ttaggtggaa acaagaaatt ttataatatt taataaaaat tttggttgag
     7861 taaggagaat tttttatggg agacctatat aaactacaaa atggtactga tattagagga
     7921 atagcgtatg aaaatgattc taaagaagtt aacctaactg tagaagaagt aaaaaaaata
     7981 gctaaagcgt ttcatatttg gttaaaagaa aagactaaga aagataaagt aactgttgcc
     8041 ataggaactg attctaggat aacaggaagc caatttagaa gtactgttat tgaaacttta
     8101 actaatgatg attgtaatgt aattgattgt gagattgcca caactccagc tatgtttatg
     8161 acaactatta tggatggata caattctgat ggggcgatta tgattacagc aagtcactta
     8221 ccttattact ataatggttt aaagtttttc acagaaagtg gaggtttaga aaaaactgat
     8281 ataaaagaga tgttggatat agctgttaaa aatgatagtg tagactatga ggaagtaaat
     8341 aaaaaaggtg aagttatagt aaaaaattta atagaagatt attctaattt attgatagat
     8401 aaaattagaa aaggtgtaaa ttcaagtaaa aattatgaaa agcctttttc tgggcttaag
     8461 atattggttg atgcaggaaa tggggctggt ggattctttg ctgaaaaagt tttacacata
     8521 ctgggagcag atacgacagg tagtcagttt ttgaacccag atggtatgtt cccaaatcat
     8581 atacctaatc ccgaaaacaa agaagcaatg gaaagcatat gtaaagctgt gttggataat
     8641 aagagcgacc taggaataat ctttgataca gatgtagaca gagctgctat tgtaggtaaa
     8701 aatggcaaac caataaataa aaatgctctt atagctgtta tatcatcaat agtattagaa
     8761 gaacatccaa aaacagctat agtaacagat tcgattactt ctgaaggact tgctaaattt
     8821 ataaatgaat tacaaggaag acatcataga tttaaaagag gatataaaaa tgtaataaat
     8881 gaagctataa gattaaatag tgaaggtgaa gagtgccatt tagctataga aacgtctgga
     8941 catgctgctt taaaagaaaa ttatttctta gatgatgggg catatttaat agctaaaatt
     9001 ttaataaaag tagcaaagct atccttggaa ggaaagacta tagaagaatt gatagaaaac
     9061 cttgaagaag ctcaagaaga gaaagaagtt agaataggca ttaataaaga agattttaga
     9121 acatatgcag aggatatatt aaaagattta gattctcatg ttaagagtat tgatggatgg
     9181 tctgtagcac caaataattt tgaaggcata agagttaatt gtgatgaatc aaatggagat
     9241 ggatggtttt tacttagaat atcactacat gaaccattat tagctttaaa tattgaatca
     9301 aataaaattg gcggagcaga acaaatttat tctaagttaa aacttttctt agaaaaatat
     9361 gatttaaaag gtctttaggg taaatagaaa gaatatattt atattgaaga ataaatgtaa
     9421 atttgtacct aagattacaa atattacaaa attgtaatct taggtaacag agatgtaact
     9481 gattatggaa acactatgtg atattataga tacagagaga tgaaatatgt gatgaattat
     9541 agagaaatat aatttaaaat gtattaatat cacaccaaaa aggtataatc aaaaaatatg
     9601 taaatataaa ccccaacatt tagaaaaaga cctctaagga ggtttttttc ttgtttaaat
     9661 aacttaaaat atatttttat attaatatat caattaactt ttctaaaagt aagcattacc
     9721 ttaaataaat cagcatctat aattatttcc atgtgtgcat tttctaattc aattatattt
     9781 tgagcaattg ataaaccaag accagagcca tctgtttttc tagatatatc accccttcta
     9841 aatctttcta ttaactcttc tggttttaga tttagtttat catttgaaat atttttaaac
     9901 gttaatatta tattttcttc ctttataaac atatctatat agaccctagt gttaggcata
     9961 gagtatttta gtatattgca aattagattc tcaaaaactc tccaagtttt tttaccatct
    10021 atcatggtaa aaacagcata ttcaggtgtt tctattataa aatctaaact gctattttga
    10081 attttgtctt caaattctac aatactttga attaaaagtt ctcttatatc ggtttttgtt
    10141 atattaaatt ctacatctcc actacttagc ttagatgctt caaatatgtc atctattaat
    10201 aattttaatc tttgtgattt tttatctaaa atatttatat agtcatttat agtctctctg
    10261 tcaaagtcat tattttttaa catatcaaca tagcttaata tagatgtaag tggagttttt
    10321 aagtcatgag atacgtttgt gataagctct gccttcatcc tttcattttt tatattagca
    10381 tctacagcag tttcaagacc gcctgttata ttatttatac ttttggctaa agtagatatt
    10441 tcatcatctc ctataacttc taatttatac tttaaattgc cattttctat attttttata
    10501 ttttttgata tgttatttat gtaattaaac ttaacagata aaaagttgta tatataaaaa
    10561 tgaaaaactg tgtttacaag tgtaaataaa attactatag ctattgcctc agcataatat
    10621 atatacataa agaatatcaa aacaagatat tggataatag cttgtacaac tatagatatt
    10681 aaaaatacta tagctaattt tttatttata gtttttttat tatacatact aagtaagttt
    10741 gaaaagaata catatgttat agacatatct tttagattat agttattata tttaatattt
    10801 tcatatatta tatagactat agatataatt ataaaattgt agattattaa attagtgttg
    10861 taaaacattt tattacttgt aaatgcatga aaccttaagg ttaatagtat caaagttaat
    10921 ataagtgtaa gttctatttt taccttagaa aaaaggttta tgacattctc taagaatttg
    10981 actttagttt gtttattttt tttatacaat gtaaaacata gtatagtcaa taaaaaactt
    11041 gataatataa tacctatgca gacaaactct aattctactt ttgacaaatg tacaggagta
    11101 ccgaaaaaat aaaaataagg gagagcaaac tctctataat aataattaat attagaaaag
    11161 ttattattca aatcataaac tgcttcaaat aatatagaaa ctaatagaaa cagtaataat
    11221 aataaaataa attttgcttt agaatcatta ataattaagt ttttcaattt tgtaaccaat
    11281 gccccacacc acctttaaat attttggttc ttttggatta atttcaatct tttctctaat
    11341 tcttctaata tgaacagcaa tagttctctc acaactataa gcatcctcat tccagacatt
    11401 ttcataaatc tcatgagaag agaaaatttt accttttcgt gacataagaa gtgtaagtat
    11461 tttatattca agtggtgtca ttttaatttc ctcaccatct aaagaaacta tttttgtatc
    11521 tgtatttagc tctaggccac cactttttag aatataatta gtatcatttg agttatttgt
    11581 attactaaaa tcacctaaat ttatatacct tctaagttga gattttactc gagcagtaag
    11641 ttctagtgga ttaaatggtt tagttatata gtcatcagca cccatattaa gacctataat
    11701 tttgtccata tcttctgatt ttgcagataa gagaattatt ggtatattcc ttttttctct
    11761 tattttaagt gtagttttaa gcccatctaa attgggcatc attacatcca ttattattaa
    11821 tttaatatca ttttcgatta gtatgtccaa tgcttcaagt ccatcatatg cctttaaaac
    11881 attaaagtta tcaggtctca aatagaactc tatagcatca actatctctc tatcatcatc
    11941 tacaaccaat atattttgat tcaatttatt aacccccttg aaattttatt tttaaccaat
    12001 ttttatttta taccaaaaag aggtattaga aaacagtaag cccgttcatt atgaaataaa
    12061 aactagtact tttaatatat tgtcaatgca tatagtaaat ttaaaagtat agacgcccat
    12121 attaatctga aggtaaaagt ttatgaaaat atctagggta atctatcaca cttcttatgt
    12181 tattttggtt tattaatttt ttaggtttaa acatatatta acaaatataa ataacaaatg
    12241 agtttcataa ttattaagaa tttataacaa atatttattt taatgtagtt taatataaac
    12301 aggtagttta atgtaggtat gtataaagca tttaataagg cttataggtt ttaatgatga
    12361 tatctttata tattttaaga gatgatgaaa ttagatatgg gtaatagttg tatgagaaaa
    12421 agtagtatag cacaaaaagt gctatatcac aaaatctcaa ataatttcct ttttataact
    12481 taaattaaag aataaatatt tcttataaat taggtgtatg aagtatagca ggaacatctt
    12541 tattatttaa taacttatca aatgatttat ggtcagactt atagccaata ccttctctat
    12601 attctaattc atcaacaatt ttgtattgtt gacaggcatt tttaaccttt tcatagtcct
    12661 cagtagaatg agctttataa actatcttat tattaaaagt tattttatca gcaaatttag
    12721 gttttacaaa atatttagca tcttttattt taccagcatt atataaattt tggataacag
    12781 aaccattttt taaatgttgt aaatggtcat ctaatttata agtatgtgct atatgagtta
    12841 catttttaaa tttttcttca aatgtaagag caattttttc catgtaattg aagtcagtat
    12901 taccatttgt atttgtttct ggtaataata ttatattttc ccttattaca ccaaggctat
    12961 taagagaagc taaaaattct ctatttctat attctgattt ttgtatattt gttaggtttt
    13021 catatttttt atatttatca aagacggata taccacatcc agtagatact aaaaccacaa
    13081 atacatgatt ttttcctctt tcttttatag cattaacaat agcactccca gcccacaaca
    13141 cttcatcatc ttgatgctga acatagaaga caacataatc tttaaatttt ttatgactat
    13201 aattcacata gctactaaaa gcggttaaaa tcaaacataa aactagaata atagatagaa
    13261 ttttatattt atttatagta aattttttat tcttaaatgt catttaatca tctcctgaaa
    13321 ggttgtctta gttatattat caaacaaagt actaaaaatt atttcttaaa tgctgaaaaa
    13381 tatagagtag attaatggaa taaaattttt ttattagttt aaaataaaga aaacatttat
    13441 gtataagact aatcaaaata ttaatgattg ataggaattg agatttaaag ggattgtaca
    13501 tttattcggg attcatttta atagaatttt actttagtac ttagaataaa agtaataact
    13561 ttttaaattt aaagagtaca catatatatt tgtgtactcc ttgatgatat gattgcttat
    13621 ttactacaaa aacagagtct ttttaaacct aaaatatttg atgcaccagc tatcgtagtt
    13681 ccataatagt aaactggttt gttaagtgat agaaaattta taattgagtt attacataat
    13741 gtacttcctg tgcaaatcac taaatcagcc caagatatta catcttcata atctcgtata
    13801 ccatcttcaa tcttaacatt atattgaata gtatccacaa attcaggatt taaatctaat
    13861 actcttgttt cgaaaaagtc cttaatatta tcaattatgg ctggttgata tcctataatt
    13921 gctacttttg gattaccaaa ttccattttt ataaactcag ggaatttttt tgcacatacc
    13981 tctggctcgt tatttttaca atgaatggtt ctatccgtct tgcctaagta tttcataact
    14041 gcatttaatg atgctataaa aagacttcta tcatgagaat tgtcatgtaa atctaatgat
    14101 aatatgtctt ttaaagaacc ttcaaatgta gatggagtag aagtaaatgc ttgtccttta
    14161 gctcctttaa agtcagcttc aagcattatt tctttaccaa ctataattgg gaaatcttta
    14221 cgcttagtat ttccaattgc ttcttttgaa gatagagatt tggacacaat acttatcata
    14281 tccatattta aattatattt atcgacttgt actaatagat aatctctcaa aatatcaaat
    14341 atattcatag aaaaaagatt ctcctttatt ttttatttct tcatttaaat caaaaatatg
    14401 atttattttt ccattgttca ttattcctat cttatgagca aaaaacattg cttcagaaat
    14461 atcatgagta acatgaatca ctgttggttt gtattttatg tagacatcct tcaaaatttt
    14521 gcttatatgt ttttttgtaa tataatccaa tgaactcata ggttcatcta aaagtaatat
    14581 atcaggttca gctattaagg cacgtgcaaa agcaactcgt tgcttttcgc ctccacttaa
    14641 atttttagga tacctatttt ttatatcttc tatctgcatt aatttagaca agtggttagc
    14701 cttaagaatc tgttcatcta taggtatttt tctcaattta agtggaaacc ttatattatc
    14761 ttcaacattc atatttggaa ataattcaaa attttgatat acaaatccta tatttctatt
    14821 ttctggaggt aagtttgata ttaaattttc tttgtatgat acatttcctt tagcagatgg
    14881 atatcttcct gctaatgttt ctagaaatag agttttacct gacccactag ctcctaataa
    14941 aacaaagtat tcatgattat taatatttaa actattttct tgtaatttaa aattaggtaa
    15001 atcaagagat aaatttttta tttttaacat attaattctt atcaacctct ctattaaaaa
    15061 atatctcaaa aagcatcaag catgtaagtg agagaaatat caaaattaca gctattccga
    15121 tagcaatgtc tatatcacca gtagacatat ttaaatatat agatgtagga ataatttcag
    15181 ttttcatacg tgtagaacct gctatcatag ctactgcacc aaattcacca agtgctcttg
    15241 accaagtcat tacagttgca gatatgattc cagtttttag atttggaaga attataaatc
    15301 taaatgcttc ccaactatta catccaaggg tacgtgcaac aaatatcatc ttttcattac
    15361 tttcatttaa tgaagtgtga agtattttaa tagtaagtgg aagatttaca aatatttgtg
    15421 ctaaaactat accttgcttt gtaaatataa aatcaagctt aaaaatctta taaatggaat
    15481 ctccaatccc cattcttcca aacaaaagta aaagagcaat tcctgatact aagtgtggaa
    15541 gagacatagg gagatttatt atctgtataa tcagtttttt aaatggtaat ttagtaatat
    15601 gtaaaaaata tgctactgga atagctaata aaaggcatac tatagtagat attaaagatg
    15661 ttttaatact aagtgttatt gcatactgta cttccactga ttttaagcat ataggaacat
    15721 aacttatccc tttaatgatt aacataagaa gtggtgatac aataaacaaa aagactatag
    15781 caaaaaatcc agtaagtaga gtagtaaaaa atttattttt caagtttaat cactacttaa
    15841 caggttcaaa accatatttt ttaaatatct gtttagcttc gtctgactct atataattta
    15901 taaatttatt agctaaatct tttttatcac tatagtttac gattcctgct gatatgagtt
    15961 ggtctatatt tttatcttgg gcaatttcta taacttctac atcttcatta ccaaagaccg
    16021 aatcttttgt ggctattgta gcatcagctt tacttgatgt taactgtaca atcatttcat
    16081 ttacagttgc agctgtagag acaacattat catttataga ttttaaatta tttttttcaa
    16141 gtattttttg tgtagtctta cctatagcat tagactcttt gtctcccaaa ataagtttaa
    16201 ctccatcttt atttaagtct tccaattttt gaatattagc tggatttcct ttaggtacaa
    16261 ctattgcagg tgtatgatgt gcaaccgttt tatgttcacc tacaagttct ttttcaaagg
    16321 cctttgaata aattggttca gaaccaacaa taaatacgtc tcctttgtga cttgtctcaa
    16381 tttgagctat tagctgtgca gaacctgcat agctatattc aactttgaca ttattttctt
    16441 tctcaaattt ttttgctatt tcatccattg gtttctttaa acctgcccct gaatagacaa
    16501 aaagagtctc tttttctttt gaatcatcag ctgttgtcac tttagtatta gattcatcat
    16561 tagctttgtt ttctttagaa ttacatgcag taatacttaa tgaaataatc atagctaaag
    16621 ctagtactga aagtttttta aacataagat attccccctt attaaattag tgtaaattct
    16681 tttttagtat atcatactaa gaaaacgatg tctaagtttt tgttatgttt tataatgttt
    16741 tgttatgttt tgttatgtat agttaagaaa aaatctattc ataatgtcat ttccataggt
    16801 agttatttta tttttaattt ttattaatac tttaaagtat tttaaattta aaaacttaaa
    16861 tcaaattaac ttattgtaga ttttttac
//
LOCUS       DAMJWS010000092        16046 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMJWS010000092 DAMJWS010000000
VERSION     DAMJWS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.095"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            88..630
                     /locus_tag="PC399_003105"
     CDS             88..630
                     /locus_tag="PC399_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2593949.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            958..2451
                     /locus_tag="PC399_003106"
     CDS             958..2451
                     /locus_tag="PC399_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2593950.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYNSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            2525..2758
                     /locus_tag="PC399_003107"
     CDS             2525..2758
                     /locus_tag="PC399_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593951.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            2914..3678
                     /locus_tag="PC399_003108"
     CDS             2914..3678
                     /locus_tag="PC399_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2593952.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(3963..7142)
                     /locus_tag="PC399_003109"
     CDS             complement(3963..7142)
                     /locus_tag="PC399_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2593953.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPREYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            7423..9600
                     /locus_tag="PC399_003110"
     CDS             7423..9600
                     /locus_tag="PC399_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2593954.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFTNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            9614..10036
                     /locus_tag="PC399_003111"
     CDS             9614..10036
                     /locus_tag="PC399_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2593955.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            10444..11247
                     /locus_tag="PC399_003112"
     CDS             10444..11247
                     /locus_tag="PC399_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2593956.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            11240..11908
                     /locus_tag="PC399_003113"
     CDS             11240..11908
                     /locus_tag="PC399_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2593957.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            12144..12806
                     /locus_tag="PC399_003114"
     CDS             12144..12806
                     /locus_tag="PC399_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2593958.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            12866..13663
                     /locus_tag="PC399_003115"
     CDS             12866..13663
                     /locus_tag="PC399_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2593959.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            13781..14971
                     /locus_tag="PC399_003116"
     CDS             13781..14971
                     /locus_tag="PC399_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2593960.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            15080..15829
                     /locus_tag="PC399_003117"
     CDS             15080..15829
                     /locus_tag="PC399_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2593961.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         6240 a         1680 c         2650 g         5476 t
ORIGIN      
        1 ttcaattaat atattattat tagtaataac tttaaaatta tattactatt atataaaaat
       61 tggcttaaaa gaaggtgaaa aaagtaaatg aaaatatgca taacagtagg tcatagtata
      121 ctaaaaggtg gtaaaagtac aggtgtaaat ggtatagttg atgaatacaa ttataataaa
      181 aaattagctc ctatgttagc tgagatgctt ataagtcaag gtaatacagt agatgtaata
      241 atttgtccag aaaggcactt tatgtcagaa agagaagaat ttttctatag agttccaaaa
      301 gtaaacagtg gtaaatacga tttattagta gagctacatt taaataaagc tgatggaaca
      361 caatgtggta cagaagtgct ttactatggc actgaaggat tagaatatgc taaaagagta
      421 tctaatagac ttggagaatt atttgaaaat agaggagcta aaaaaagaga aaatttatat
      481 atattgagaa atacaaatcc agttgcaata cagattgaaa gttttttctg tgataatgtg
      541 aatgattgta acaaagcaaa tgaatcaggt tatgattata tagcacgatt gattacagaa
      601 ggtatattga ataaagatat aaatatgtga gattatggtg atttaggtat tgattaatac
      661 cttttaatat tagatataaa taaaaataca atgtattagt ataatttaaa ctttcaaaag
      721 atagattagt ttgattattg acaagagata cctccttgaa aatattgatt tatcagtata
      781 tttaaggagg tatttaaata taaaaattta attaatttgc tactaaacta taggtttata
      841 gagtatatat ttttatcttc agtctaataa ataatataaa aatagtagaa ccttgtatta
      901 taataatata atgtagaaaa atatatagat aagataaata taataaggtg gtaatatatg
      961 aaatatacat ttgaaaaatt taagttaaat gaaaagatac ttaagtcatt gaagagttta
     1021 ggatataata taccttctag agtacaaaga gaagttattc caaaattatt aaaaggtcaa
     1081 aacttagtag ttaggtcaaa aacaggaagt ggaaagacag caagttttgc aataccttta
     1141 tgcgagaata taaatgtaga ttacaataat attcaagctc ttatagtggt tccaacaaga
     1201 gaattagcct tgcaagtaaa agatgagata tcagatatag ggagactaaa gaaggtgaga
     1261 tgtagtgcta tatttggtaa acaatctata aaagaccaaa tagctgaatt gaaacaaagg
     1321 gttcatattg ttgtagctac tccaggcaga atacttgacc atataaatag aggttctatt
     1381 aagttggaaa atgtaaagta tttagtaata gatgaggcag ataaaatgtt taataaaggt
     1441 tttgttgaac aaatggaaaa aatattatta aatttaccta aggagaaaat agtaagtctt
     1501 ttttcagcta caatagatga agagataaaa tatatatgtg aaaaatatat gctagattac
     1561 agtgttataa atatagaaga aaatgagagc gatactaatc aaaaaacaag acaaatagat
     1621 gacaaaataa tcaaggcaaa tggaagagag aagtacatcc ttttaaaaga attaatatac
     1681 agcgaaaatc caaagagtgt gataatattt tgtaatacca aagagaaagt atctaaatta
     1741 tataacaaga tgagtaaaga aggtttttta ataagagagt tacatgcaga tttatcacaa
     1801 gaaagaagaa tttttgtaat taaggatttt aaaaaccaaa aatttaatgt attagttagt
     1861 agtgatgtag cgtccagagg aatacatatt gatgatattt ctttagtaat aaattatgat
     1921 gtgccacaag ataaagaaaa ctatatacat agaataggta gaactgggag aaaaggaaat
     1981 agtggaaagg caattactat agtaactgaa aaggatgaaa aatatataga gaatatagaa
     2041 acgtatatag gatataaaat aaatgaatta aaagatatag aaaaaagtag aattacacat
     2101 ggcaaaattt tgtttgaaga atattcaaaa ggaatcctaa aaaaagtatc aaagagaaaa
     2161 aaagttgata aaaattctta caatagtaaa gatataaaac taaatataga aagtgaagtt
     2221 gttaaattat atttaaatgc tggtaagaag aagaaaataa gagttttaga tatagttggt
     2281 gcatttagta atataaaagg tataactaat gatgatatag gtgtaattga agttcaagat
     2341 ttatgttcat atgtagatat attaaattat aagggagatt tgatattaaa aaaatataag
     2401 gagataccta taaagaaaaa aatggttaaa gtgaaaagag atatacgata atttagtcaa
     2461 tattgatgaa ttgctttgtt atcgaaatga agttgcaaaa aacacaatag aagttgttaa
     2521 ggatttgaaa gctgaagata tgaaacgaaa ggtctctcca attgacttag aaaggatttt
     2581 ggtgactgga ggagtaataa agcatgaaga ttctatttgg ttgttggact tttgggggaa
     2641 taaagatatt gctggaattt tgcttatgcc tcctactcgt catgttatac ttcatttgaa
     2701 tgattgttgt aaatggaagg aatattttcg tacaaaaaag aaattttaca gaagttaaat
     2761 tataataaat tagacaaaca atagataaat tagcaaggaa tgttgatttt atctacattc
     2821 taagttatac tatttttaag tggctaatgg aatagtcatt ttaaatataa gtaataaata
     2881 tatacctata gggatattaa aggggggaaa tatatggcat attttaacta taacgacaaa
     2941 cagtgctact ataatgaaat tggggatggt acaccattac tttttttaca tggaaataca
     3001 gcctcttcca agatgtttaa tgatataatc gatttttata aagatgaata taaagtaata
     3061 ttaattgatt ttgtaggtca tggaaaatct caaatggtcg ataaattttc tgctgactta
     3121 tggtttgatg aggctatgca agtgatatgt tttttagaag caatgaacta taaaaaagta
     3181 aacataattg gtagtagtgg aggagctctt gttgcattaa atgttgcact tgagagacct
     3241 gatttagtga ataaggtaat tgcagatagt tttgaaggag aagttcctct agagtcattt
     3301 gtacaaaatg tgaaaataga gcgtgaagca tctaagcaag atgatggtgc aaaagctttt
     3361 tatatttata atcaaggaga aaactgggaa aaagttgtag ataatgatac agaagctatt
     3421 tttgaacatt ataaaactat aggaaaattt tttcataagc ctctagaaac tatgcaacca
     3481 gaggtgcttt taacaggaag tagagaagat gagtttgtat ctctaatatc tagtgatttt
     3541 tttgaaaata ctttttcatt acttcttgaa aagattaaaa atggtaaaat gtatttgttt
     3601 gataaaggtg gtcatcctgc aattttatcg aatggattag atttttcaaa tgtagcaaag
     3661 aaatttttag aggaataatt taaccaaaat ttataataat gaaaactgta aaatggattg
     3721 caaaacttac aaaagtgagt gtacatatac ttcattatta tgatgaaata gtattgttaa
     3781 aacaaaatat tctaatagtc aagttattac atttgtatat gcaatatgta tagaaagaga
     3841 tatcattaga ttgtaaagtt gggattaaat aacaatatta aattttattt attcagatat
     3901 tttagcttaa ctatataaat gttagaacaa atttatatag ttaagctgta ggattaaata
     3961 atttagttaa ataagtttat tatctcctca tcagatagac tttttaatac accttcatta
     4021 gaaagttctc catttataaa ttgatttata agctcttttt tactttcttg taattttatt
     4081 attttttctt ctatagtccc tttagcaatc aattttataa cttgtactga atttttttga
     4141 ccaaatctat gagctctatc actagcttga ttttctactg atggattcca ccatgggtca
     4201 aagtgaatta ccatgtcagc agatgtaaga tttaaaccag ttccgcctgc ttttaaagat
     4261 attaaaaaaa cttttttatc catactatta ttgaactcat ctacaagttc aagtctttct
     4321 ttggcattag ttttaccatc tatataatgg tatttaattt tatacttatc aagttcctta
     4381 cttatgttct taagcacaga agtaaattgt gaaaacaata atattttgtg gttttcgttt
     4441 atactatccc taagtatttc taaacatgtt tcaatttttg aacttttttt attgtaatct
     4501 tttactacta tacttgggtc taaacaaagt tgtcttagtt tagttaaata agaaaatata
     4561 actattttat ctttctttag atttttatct ttcattttat cttgtatatc ttttgaatat
     4621 acactatata tctttttctg ctctttattc aattcaacaa aaaagttttt ttcaatctta
     4681 tctggaagtt ctttcataac ttgtttttta cttcttctaa gaataaaagg ttttatcaat
     4741 tttttcaggt ttttaacatt atcttcttta tttataaaca attcctgaaa ctttgcttta
     4801 ctatatagat agccaggcat tataaaatca aatatagacc ataattctag tagattattt
     4861 tccataggtg taccagtgag tgcaaattta ttttctgcat ttatactttt tacagactca
     4921 gttactaaag ctactggatt ttttatattt tgagcttcat ctaatataca ataatcaaac
     4981 tttatttcac tatatttttc taaatcattt ctaagagtac catatgttgt aagtattaca
     5041 tcaaaatttt ctaattccat aaaacatttt tctctatctc ttttatttcc atgtatcaat
     5101 aaaactttta tatctggagc aaacttttca aattcatttt tccaattata aatgagtgat
     5161 gtcggtgtaa caataagact ttttttatta gataatgata ataaaaatgc aatagtctgt
     5221 attgttttac caagacccat ttcatcagct aatataccac caaatttata ataatctaaa
     5281 actttaaacc aattgaggcc atttatttga tagtcacgaa ggtttgcatt taaatttgcg
     5341 ggaatttcta aatttatgct atcaaaattt ctaaattttt cacatatact gtctagttct
     5401 tttttaccat ctataaactt taaattttta ctacgtatta catcatttat atatagagct
     5461 ttatttttat gaattttatt attttttgat ttatccatta tatctagatt ttcaactaat
     5521 tcaaaaaaat cttttgtttc tctttctgat aaatctataa agtttccatc tttaagttta
     5581 taaaaagtac gtttttcttg aaaagctttc aaaatatcct tgtactctct tggatttata
     5641 tcagatatat taaaattaaa ttctaggtaa ttctggttag tctgattgat tgaagcttga
     5701 attgatgaag cgttatatat ttttttcaat ttaaacttct cagaataata tatatctcca
     5761 aattccttta gagattctaa ttgtaaatct aaaaatgtaa ataggtcatg gtcagttcct
     5821 ttaaacacat aatgattatt ttcctttgta aattgtgaag aagtaagttt attttcaata
     5881 gtagcttcct tttgtatgtt ggggagcaca aattttttat cttcatcatc atatatatac
     5941 tttacatcac aacatatgtt gccatcaatc atatcaaagt agaactcaga tttaaaatct
     6001 tttgttatat tattttttat gttatcatct aaatgaactt cttgagatat agattttaat
     6061 ctaggaacta gcaatcctaa aacatcctta gaatcctctt tatcaaattt taactcttta
     6121 gtctttgata atatttgata tatttttgaa taatcatgag cttttttatg tgataataaa
     6181 tagatattgc catcatataa aagtacatca ttttttttag taagtggaac aggtagtgaa
     6241 tctttagaat ataagactat tttatcttct atatttttaa aatcaaacct tacaggtaga
     6301 gtatcattta atatctttgg atgatattcc tctttagaaa aaataagggt aaaatccttg
     6361 tacttaatag catttaaaaa ttgttttaaa gagcttgcat ttatctccat aaattttttt
     6421 gaatgtgatg aagaaaagtt gtatgaaaga tttgtcccat attcttctat aatatctact
     6481 aacttctcat cttcatcagc aaaataatga gtgttggggt tgtaagtaaa tttcttccca
     6541 aaatctaatt catatccatt aagttttgaa tatgcaaatt cagtaatgtt ttttaaaatg
     6601 tacattttat caattcctat tttaaattca gcaattgctt tatttgaatc cacatttatc
     6661 aatacatcta aattaaccct ttctctttta gtattgtcta acaaagaaag taattgcttg
     6721 cttaaatcta agctggtttt agaatttgta ttagaaagct tatccttatc tgcacctaat
     6781 atatctatta gaataggagg aacatctttt ttatttttta tttgttttaa gctttttatc
     6841 acaatagcaa ctatatgctt acatataaaa atacttttgc cctttgattg acagtcagcg
     6901 caactacaat cattttcaat tatcttaaag tgctttatat ctatagttat tgaattaaaa
     6961 taaaagctat aattatcttc agaattgact ccaccacgaa atgtcaacag gttattttgt
     7021 aggtcatcaa tatctatgtc tatataatca actaattgat tagtataata ttttacacct
     7081 cttgaccatc ttgatggttc tgtatttctt cttattagtt tatatatcat atctattttc
     7141 atattattca ccttacctca ctgtatatat tataatctaa atgcattcaa tttacactct
     7201 aaatttaaga ctaaaattaa ggcagatatg aatagctttt atctatcaca atatatgtgt
     7261 aaataacaat ttactattag atttttattg aattatctta taaactatga atgaaaaata
     7321 attatataaa tagattatta ataaatataa aatcttatta ttaaaaatat tcttagaaat
     7381 tacactatat aaaatataaa gtatgatatt tgaataaaaa gaatgtataa taagtataat
     7441 aatttgtatt tattattggg aaaggaaaat gttaatatgg atagttatag acaacttaaa
     7501 gctaaaatac cctgtaagga aacaggtata gaaataaaga ggactttatg tgatatttgt
     7561 tctccaggag tacattgtgg aattgatgca tatgttaaag atggtaaagt aataaaaata
     7621 gaaggtacaa aagaacatcc aatgaataaa gggttgttat gtaccaaagg tctttgcaat
     7681 agagaatata tctatagaga agatagaata aaaacacctc taaaaagaat tggaaaaaga
     7741 ggtgaaggaa aatttaaacc aatatcatgg gatgaagctt atagagaaat agcagaaaaa
     7801 ttaaatagag taaaatctga gtatggagca aagcatgtag ctttttttac gggttatgga
     7861 aagtggtata gacctttttt acaaaggttt gcatcttcat ttggttcacc taattatggt
     7921 acagaatcta gttcatgttc aacatcagca cagatggctg gaaatgttgc tactggaaga
     7981 aactttacaa tgcaagatat ggagaatgca aaaacttatt tagggtgggg atttaatcca
     8041 tactactcta aatttttaaa tagtgtgaat ctaataaaat taaaagaaaa aggtctaaaa
     8101 tttataattg tagaccctag aataacaccg acaactcaaa aactagcaga tttacattta
     8161 agaattaaac ctggaactga tggagcttta gctcttggaa tggcgaatct atttatacaa
     8221 aaggatttaa tagatagaga atacatagaa aagcatgtac atggatttaa tgaatataaa
     8281 gaatatgtaa aattatttga tttagatact gtaagtagaa ttacaggagt ttctaaagat
     8341 aatatagtaa aagctgtcga gatgttgtca caaggtccat tttgtataaa tgaaagtgca
     8401 gctccaatag ttcaccatca aaatggattt caaaattata gagcaatgat gtctttatcc
     8461 gctataacag gtaattatga tagaataggt ggaaacatac caaaacaaga ttactttgct
     8521 ggtaagatag gtggattcca aacactagaa aaagagtttg taaaagaagt tctacctaaa
     8581 aatataaatg aaagaattgg tggagaaaga tttcctctat ggaatgaaat ggtgactcaa
     8641 tttcaagcta tggatttacc taggcaaata gaagaaaaga caccatatga aataagagct
     8701 ttgttttgtc atgggataaa tgctagaatg tttactaatt cagggagact ttttaaagcg
     8761 ttgaatagtg tagatttttt tgtgaatata gatttattta tgacagatac atcaaaatat
     8821 gcagatatac ttttacctgc atgtacatca tttgagagag aagaatgtaa gagttatcca
     8881 ggagggtata ttacatatac caaaaaagtt attgataaat tatacgattc aaaatcagat
     8941 gtagaaatac tttcagattt agcaaatgct atgaatatag atgatgattt attaaaagca
     9001 ggttatgaag caagtataag acatatgttt aaaaatacat gtgtagatgt agataaactt
     9061 aaagaaagtg atttaccatt aaaatgtaaa gattttaagc catatatagt aggttcatat
     9121 acaagagaag ggtatgatac tagcacttct aggtttgaat taaaatctac aattattgaa
     9181 aaatataaag attttgggct ggatgcacta ccaacatata gaggatttaa tgataatata
     9241 gatgatgaat atactcattt attgacatca ggagcaagaa tttcaaatgc actacattca
     9301 agacttcatg acatcaaact ttcaagaata ttaaaaaatg aagctagttg tgatatttct
     9361 agcttagatg caaaagaatt gggtgttatc agtggagatt atataaaaat aactactaag
     9421 ataggaagtt tggatgtaaa agtcaatgtt actgatagag tcttatataa gaatatacat
     9481 atgtatcatg gatatagaga agctgatata aatagtatta tttgtgataa ttttgaccct
     9541 tatagtggat tccctgcgta tagaagttgt aagtgtaaga ttgaaaaaat gaatagataa
     9601 atggagagat ataatgaaaa gtttatttaa atttaataaa gaattatgta cagcatgtgg
     9661 agcatgtttg cttgcatgta tagaccaaaa tgataataat atagacaatg gtgaactttt
     9721 tagacatgta tttacaatag aggaaggtga aaatatagaa tatttttcat tagcatgtat
     9781 acattgtaat gagccaaaat gtttaggtgt atgtcctaaa aattgtttta aaaaagaaga
     9841 aggattcatt gtattagaca atcaaaactg tatagggtgt aaactttgtg aaaaagcttg
     9901 tgaatatgga gccttaattt atggaaatga tggtaaggca aataagtgta atggttgtat
     9961 tgagagatat aagtgtggta tggattcacc ttgtgtaatg gtatgtccta caggagcatt
    10021 aaaatttaat aaataaatta aatatagaga atgacttata cttaggtata agatattttt
    10081 acaaaaagct gtattgagtt tgttataatg aaaactcaat atagcttttt tatggatata
    10141 gttggatact ataatgtgat tcttagtaat agttttttgc tattggacaa agactgtaaa
    10201 acatattaaa cttatgttgt tcaaagtcga agtataatat aataactaga aaattggttt
    10261 atagtgctat aaaagcaaat ttagtaaggt ataaaatcag aggcaatcct ttactataat
    10321 atcaatattt gggagttctg ttattgcaga gctattaaac aattataatg gtacaagtga
    10381 ttcaaccttt gaaaatgtat tatataaaaa attaaaataa gattaaaaag gagagaataa
    10441 attatgaaaa aacttaaatt aattattatg tttgccttgg tatcagcact gttagttaca
    10501 ggatgttcat caggaaataa taaaaataaa gaagaacaaa aagttataag agttgcaaca
    10561 tcaggaacat attacccttt tgcttttaaa gataaagata aacttaaagg gtttgaagta
    10621 gatttttggg atgaatttgc taaacgaact aattgtaaag ttgaatgggt attaactgat
    10681 tttagtggat tatttggatt attagaagct gataaagcag atgttgtatc tgctcaactt
    10741 actgcaacac ctgaaagaga gaaaaaatat gctttttcag atgcttataa ttatagtgga
    10801 acaaacatta ttgttagaga agatgatact agtataaaat caatagatga tttagttggc
    10861 aagaaagtag gtgtgggtac tggagcagta gcaaatgaaa tattaaaaga aaaataccca
    10921 aatgatgaaa taaaaatagt aaattattca agtgcgactc ttaaagggaa ttatcaagac
    10981 ctagaaatgg gaagattaga tgcagtagtt gcgcaggatg ttgaggcttt aatagctata
    11041 aaagaacaaa aattaaatct taaaatgata gaacctccaa ttcaatttgg tgcctgttct
    11101 tttgcgcttc aaaaaaatga aaaaggtgaa aaatggacaa aagaaattaa cgaagtaatt
    11161 gaagagatgt ataaagatgg gactttgact aaaatatctg aaaactggct aggaaaagac
    11221 attactaagg agcctattaa tgagtaattt taattttgaa ttttgtctaa gtatatttcc
    11281 atttttgatt aaagcattgg gaataacaat actaatatct attgttgcat tttttttatc
    11341 aatattatta ggtgtaatac ttgccttttt cacacagaca aaatcaaaag ttttacaata
    11401 cataacaaga gtgtatgttt cattctttag aggaacacca cttttaattc aaatattttt
    11461 cttttatttt ggatttccaa tgatattttc ttttatgaaa gattgttcac cttatttagc
    11521 aatagtaatt tgtttaggtg taaattcatc tgcatatatg gctgaaatta tacgagcatc
    11581 tattgagagt gttgataaag gacaacagga agcatgttat gcttttgggt taagtaaata
    11641 tcagtcaatg gttaaaataa ttctacctca agcagctaca tctgcagtac ctcctattgt
    11701 aagctgtaca ctggacataa ttaaaggttc ttctttaggt ttaactatag gaatacaaga
    11761 tttaatggga gtagctcaag tagaggctgc ttcaagtttt aaatattttg agatatatat
    11821 gataacgata ttaatttact gggcagtatc tataatattg agtaatatac aaagcaaagt
    11881 tgaaaaaaga ataagaagag catattagat ttcaatattt aaaaatggga tgatatagat
    11941 atattattac ttgtaaataa atttttaatt ttagaaaaat aaacttagta atctaaatta
    12001 ttgagaaaat atagtatcaa aatggactgt ataatcaagt taaaaaatag tttttctatt
    12061 gaaaaaataa ataaaattct attgtaattt aatatgcgat aaagttttat aaaaaacaac
    12121 attaagctag ggggacttat tttatggctt ttaatatgaa atatttttta gaattatttc
    12181 caacaataat gaaatcgttc aatgtaactg tgattttggc aataagttca ttagtatttt
    12241 cattaattat aggtacaata gtagcgttat catcttatta caaagtgaaa gtattgaatc
    12301 aaatatgtaa agtatatgta tctattttta gaggtacacc tcttatgcca cagttattct
    12361 ttttatattt tggactagca tatatgagtg attttgttaa aaatatggac ccagtatttg
    12421 caacatctat agtacttagt ttaaatatgg gagcttatat gtctgaaacg ataagggcat
    12481 ctatactttc aattgataca ggtcagatag aagcagcata tgcaatggga ttaacaaatt
    12541 tacaaacaat gaaaaggata gtgataccac aggccgtaag agttgcactg ccatcattat
    12601 tcaataattt tatagattta attaaagggt cttctgtagc ttttgttgtt ggagtaaatg
    12661 atataatggg tgctgccaaa tcacaaggag cgttgagttt taagtttttt gaagtgtatg
    12721 gagcagttat ggtaatttac tggtgtataa taactccttt aggttggggg caaaaaaaat
    12781 tagaaaagaa aataagcaat tattaagtta ttaagaaaat acataaaagt atttttatat
    12841 aaaaaatggt gtgaggagaa gagatatgat aaaaagaaat attataaaat gtttagtggc
    12901 agttttatcg ttaacgtttg taattactgg ttgttcaaaa aaagaggaag taaagaatga
    12961 aaaagttata actgtagctt catctaattc agctgaccca tattcttatg ttgataatgg
    13021 tgagcataag ggttttgaag ttgacatgtg gaaagagatt ggtaaaagaa gtggatataa
    13081 gataaaaatg gaaccaacag gttttagttc tatatttggt atgttagatt ctggtaaagt
    13141 tgatgtggca gccaacttct ttggaatgtc taaagaaaga ttagaaaaat acgatgcttc
    13201 tataccttat ggttctgatt ctgtgtgtat agctgtaaaa gatggaaata acgaaattaa
    13261 taagcttgaa gatttaaagg gaaaagttgt agcagtttca gaaggttctc aaggtcaaca
    13321 ggttgcaact agtgttaata aagataattt gtttcaagaa aaggtttacg gtgatggaac
    13381 tagtggtctt caagatttag atttgggaag agttagtgca tggattgatg cagagcttac
    13441 tattgtggga gacgctaaaa aagctaatat gaaagtaaga gtattagata aaaaattatc
    13501 agctactaat atagcatact tctttaagaa aaacgatgat aaatctaaaa cgataaaaga
    13561 agatgtaaac aaagcaatag aagaaatgct tgcagatgga acagtgaaaa aaatatctga
    13621 aaaatggttt ggaatggatg taagtgctga tatacaaaaa taaatacttt aaaattaaaa
    13681 tgtaaaactg tgataactat agataagtat actaaattta tctatagtta tctgtgtatt
    13741 acaatgtatt tagattagtc tttataaatc aaaatgagtt atggaggtat tgcaaatgat
    13801 tgaaactaaa ataaaatcca ttgcactaaa aaatagagaa aaaattataa aaataagacg
    13861 acagatacac agtaatccag agttagcatt taaagaatat aaaacatcta aacttataaa
    13921 agaagagcta aataaactta atatagaata cataaatatt gctggtacag gagtattagc
    13981 tactataaaa ggaaaaaata atggtggaaa aactatatta ctcagagcgg atatggatgc
    14041 tcttccaatc aaagaggaaa atgatttaga atttaaatca ataaatgata atatgcatgc
    14101 ttgtgggcat gatgcacatg tttcatggtt gttaggaaca gcaatgattt taaatgacat
    14161 aaaagaagaa ttgaatggaa atgtaaaatt actatttcaa cctggcgaag aaaaaggtgg
    14221 ttctgatatt gtaattaaag aaaatgtact tgaaggtgtg gatgcattag cgactggtca
    14281 ttgttggcca actattgagt caggaaaaat tggaatcgct agaaattgtg ctatggcagc
    14341 gactaatact tttgaaataa caataattgg caagggtggg catggagcag agcctcataa
    14401 ttgtatagac ccaattgcag ttggaaatgc agtatattca tccattcaac agatagtttc
    14461 aagaaaaatt gacccagttg tacctgtagt agtgtctgtt tgttctttta attctggtgt
    14521 ttctaaaaat ataataccag atgtatgtac acttcaaggg actattagag cgataagcaa
    14581 ggaacaagta attgaagtaa gtaaaattct agaatatata gtaaaaaatg tatgtaaagc
    14641 acatggagca gattgcaaat ttgaaaagag tacaggagga gatgctgtta taaatgacaa
    14701 aaacatgata gagataggaa gagaaagtgc ttgcaaaata cttggagctg aaaatgttga
    14761 aataattgat tttcctgcaa tgacaggtga ggattttgct atttatatga aagaaaaacc
    14821 tggtttattt atgtatatag gtgtaggaga taaagaaaaa aatataaatt acagattgca
    14881 tagtaacaag tttaatatag atgaaaaatg tcttagtata gcaccttcat tattttcaca
    14941 acttacagta gattatttat gtcaactata gagtacattc ataaaaatat atttagccta
    15001 tttttaaatt taatgctagt atattaattt tgatgtagaa catattattt attatattta
    15061 tttaaaaagg tggataacaa tgataaaagt agaaaattta cataaaagtt ttaaagggac
    15121 agaagttctt aaaggtataa atttagagat aagtcaaagt gaagtagtag ctatacttgg
    15181 atcatctgga acaggtaaat ctacgttact tagatgttta aattatttag ttgagcctac
    15241 aaaaggaaag ataatgattg gtgatgtaaa agtagatgta gaaaaagcaa caaaaaaaga
    15301 tataaccaat ttaagaaaac atacatctat ggtgtttcaa aattataact tatttaaaaa
    15361 taaaacagca ttagaaaatg taatggaggc attgatagta gttcataaaa agagtaagaa
    15421 agaagctaga gaaatatctt taaaattaat agataaagta ggtatgttgg ataggaaaga
    15481 tttttatcca tcaaagttgt ctgggggaca gcaacaaagg ataggtatag cgagagcgat
    15541 ggcagtaaat ccaaatgtaa tattatttga tgagcctaca tctgcacttg acccagaact
    15601 tgttggagaa gttttaagcg ttataaaatc tcttgcaaaa gaaggtataa cgatgcttat
    15661 tgtaactcac gaaataaatt ttgcaaaaga agttgctgat aaaattatat ttatggataa
    15721 tggtgtaata gctgaagaag gaactcctga tgaaatattt aataatccta aaaatgaaag
    15781 aacagcaaaa tttttaaata tcattaggaa agaagaagta tttagttaaa agtgtataaa
    15841 ttaagcatta gaagatatgt aagatacccc cattaaattt ataagttaga taataaattt
    15901 aatgggagta tatttttatt attagattta ttatttaatt taaaattaga aatgtaacta
    15961 tttgaaaatt ctttttaaaa gtatatcaaa atatcaaata cataaaaaaa taatatttga
    16021 caaagtatca tatgtgttta caaaat
//
LOCUS       DAMJWS010000093        15757 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMJWS010000093 DAMJWS010000000
VERSION     DAMJWS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.096"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            134..1324
                     /locus_tag="PC399_003118"
     CDS             134..1324
                     /locus_tag="PC399_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2593962.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1494..2456
                     /locus_tag="PC399_003119"
     CDS             1494..2456
                     /locus_tag="PC399_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2593963.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2578..3240)
                     /gene="zmp1"
                     /locus_tag="PC399_003120"
     CDS             complement(2578..3240)
                     /gene="zmp1"
                     /locus_tag="PC399_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF2593964.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYAEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3340..3425)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3611..6529)
                     /locus_tag="PC399_003121"
     CDS             complement(3611..6529)
                     /locus_tag="PC399_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2593965.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6647..6732)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6859..8037)
                     /locus_tag="PC399_003122"
     CDS             complement(6859..8037)
                     /locus_tag="PC399_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2593966.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            8412..8561
                     /locus_tag="PC399_003123"
     CDS             8412..8561
                     /locus_tag="PC399_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2593967.1"
                     /translation="MFFISYIYTKSPIEFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            complement(8662..11436)
                     /locus_tag="PC399_003124"
     CDS             complement(8662..11436)
                     /locus_tag="PC399_003124"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2593968.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11608..12864)
                     /locus_tag="PC399_003125"
     CDS             complement(11608..12864)
                     /locus_tag="PC399_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2593969.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12936..14438)
                     /locus_tag="PC399_003126"
     CDS             complement(12936..14438)
                     /locus_tag="PC399_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2593970.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(14529..15719)
                     /locus_tag="PC399_003127"
     CDS             complement(14529..15719)
                     /locus_tag="PC399_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2593971.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         4934 a         2736 c         1926 g         6161 t
ORIGIN      
        1 tactgtacat aaatatattt tatataaaag tttatatttt caaacaagta atttttcgaa
       61 aattgtctaa attcagtaaa aatatggtaa tatatacata agcaatataa atctataaaa
      121 aggagtgtta catatgatat ctaataaaat gcaaacttta gttgcaaata gttctgttat
      181 aagagctatg tttgaagaag gaaagaaact ttctgatatt tatggtgagg aaaatgtttt
      241 tgattttagt ataggaaatc caagtgttga acctccagag actattaaag cagttataaa
      301 tgatatttta aatgaagaat ctccaaattt agttcatgga tatatgaaca actcaggata
      361 tgaggatgta agagatgcta ttgctgaaca cataaataaa aaagatggat taaatcttac
      421 aagagaaaat ttaattatga cttgtggtgc tgctggtggt ctaaatatta ttttaaaaac
      481 tcttttaaat cctggtgatg aagttattgc ttttgcaccg tattttggtg agtataaaaa
      541 ttatacagaa aactatgatg gaaaattaat agaagttcct acaaatatag agacttttga
      601 gccagattta gatgctctaa aaaatgctat tactccaaaa acaagagctt taataataaa
      661 cactccaaat aatcctactg gtgtcatata ttctgaagaa ttattaaaaa atcttggaga
      721 attattggat tcaaaacaaa aagaatttaa tacaagcata tacctaattt ctgatgagcc
      781 atatagagaa ataatttatg atggtgccaa agttccttgt gtactaaaat actatagaaa
      841 ttcatttata ggatactctt atagtaaatc attatctcta cctggtgaac gtataggata
      901 tattgttgct aatgggcaaa tggatgattt tgatgatgtt atgtcttcat taaatgttgc
      961 aaatagaata cttggatttg taaatgctcc ttctcttttt caaagggtta ttgcaagaag
     1021 tttagatgca gaagttgatg taaatattta taaaaagaat tttgaccttc tatataatag
     1081 tttaatcgat atgggatatt cttgtgttaa acctaatggt acattctatt tgttcccaaa
     1141 agctccaatt gaagatgata aaaaattctg caatgatgca aaacaattta atttattatt
     1201 agtaccaggt tcttcttttg gttgtccagg tcattttaga gtatcatatt gtgtatctta
     1261 tgataaagtt aaaagttctt taccagcatt tgaaaaatta gccaaattat ataatttaaa
     1321 ataactaaat caaagtaatt caaataatta actaccttag tatagaatac ttacattcta
     1381 tattaaggta gtttataatt ttatgtacta tattttatac ataccatatt attccaatat
     1441 gcttgaatat tcttatctat agtactaata taaattacaa ggaggtgttt gacttgaaaa
     1501 aaggttatgt ttatataata ctgactacct tattttttag ttcaatggaa attagtctaa
     1561 aaactgtcac taatgacttt aatcccttac aaataacttt atcaagattt tttgttggcg
     1621 gtttagtctt actacctttt gctgtaaaaa gattaagagc tcttagtctt tctattacaa
     1681 aatcagactt aaaatacttt gcatttttag gttttatgtg tgtagtagta agtatgtcat
     1741 tgtatcaact tgctgttcta aacacaaagg catctgtagt tgctgttttg ttcagttgta
     1801 atccagtctt tgtaatgctt ttagcttact taattttgaa ggaaaaaata tataggcaca
     1861 atgtaatttc acttatatta gaagttcttg gaattatagt tataataaat ccattgcata
     1921 caaaactaac tcttagtgga atattcttaa cattatcttc tgctattatt ttttcaatgt
     1981 atactgtatt tggtaaaaga aaaaccttaa aatttggtgg aatagttgta acctgcttta
     2041 gctttatatt tggtagtttg gaaatgttga tattggtttt actcactaaa attaatttta
     2101 tcgcagatat attaaataaa aataagcttc atgtatttgc aaatatccct atttttagtg
     2161 gatatacctt acacaatatg cccattatga tttatgtatt cgtatttgtt acaggtgttg
     2221 gttatgcgct ttactttatg gcaatggaag ctacatctac tagtcttaca tctttagtat
     2281 tcttctttaa accagtaata tctccaatac tagcattatt aatacttaaa gaaattatcc
     2341 ctataaacat ggttatagga attttattga tagtaattgg ttcaataatt tctattatac
     2401 ctacgataat tactcaaatg cataataaac acgttgaagc cttgtataag gagtagtaat
     2461 tgataatcta tcatagttat acataagaca aaatttatta gatttaaacg caatataaaa
     2521 gagatgctat tataatagca tctctttatt tatatttatt ttaaatttat taaaactcta
     2581 tttagctaaa ttttgcaaaa agctataagt ttgtggacaa gctgatttta acttactatt
     2641 tgtatcttga tttaaatagt aatatgcaaa tgattctgca aaaaattctt ctgcatatac
     2701 acctaagtag tttacattac ctaaagaacg accttcttta gcaaatattt gtttgaattg
     2761 tgctgattta gatatatcat ttaatactat atggtctatt gcatgtgctg tttcatgtaa
     2821 ttctaaattt attgcatcat gccctttacc tttgttacta aatcctattc taagagctac
     2881 tgtacttcca cctaaacctg gaacatcatc ccaagttttt ccagtacctt cccatccctt
     2941 aggaactaca ccttttaaat aagcatattc tttttcatct gttaactttc cacttaataa
     3001 ttttattttt aagttatttt gctttaatgc atttaaatac tttccatcta tattagctaa
     3061 tctatttacc atagcattag cttcgttttt atcgtaattt ccagttggaa aaactactat
     3121 ttgactaagt gtgtctttat tttgttgtat agtagtacta tctgcatgtg cacttactgg
     3181 aactgaactt attaagaata ttgatattat agctattaat aattttttac ttggtctcat
     3241 attattccct cctttgagtt aggccgggag taataaattt caaaataaaa aagataccca
     3301 agagtatctt tgtatataca aaaagaaatt tttactcttt tcggcaactg gctggcatgg
     3361 tttactaaac tttagcccct atagctttgc gtgactaggt ttccctagtt ttgcctcatt
     3421 taattttata tctatattat agcttatatc tttttaattg tctatatccc tttggaactt
     3481 ttttctaaaa agttattatt tttttacata aatcttcaaa aaaaaatatc ttctaacaat
     3541 attttcatta ttaaatattg ttttagaaga tattttttaa tataacttat attttttata
     3601 atgttagggt ctaatttgta tttttatttc ttcttaatac gataagtcct acaatagctc
     3661 ctataaccaa aagtatttct ccaataattg tagtactatc ccctgttttt ggtggtactg
     3721 gtggatttac caaagtatca tctttaactt tattattttt ttcttctgac actgatggct
     3781 ttactacttc tggtattgga ataatctgtt taacaggagt ttctatcatc tcttctgatg
     3841 gtggtactgg cggattcaat acctctggta ttggcggatt tattatatct tctgatggtg
     3901 gtattggtgg atttataact atagtatcct ctgtatcaga tgaacttgat ggtttccttg
     3961 gtttatctgt gcttggtggt actggtggat ttactattgg ttcatctgta tttggtggtg
     4021 ctggtggatt tactggtgga ttttcttgtc ttatgttttc tatatttcca atatccacta
     4081 ttctaccaat atctttatta ccaatttcaa ccttaatttt atccttcagt ttttcatatc
     4141 cacttggagc cattatctcc actaaataat atgttcctgg tttttttata ctagattgaa
     4201 ttataccatc ttcatcacta actaaatcct taactactat atttccaaat gcatcttcta
     4261 aattgaactt agcacctgaa agtacatttt tatctaaagc atccactttt ttagcttcaa
     4321 aagcataacc ttgcttagta gtctctactc ctggaggact tgaaccatta atctgagcca
     4381 cattttgtat agtctcccca acttttacat ttgtgaagtc tgcatcaaat gtaacccaac
     4441 tttgcacagg ttccccttct tttatctgtt taaattcgta tttatttact aatcttattg
     4501 tattttttcc atctccagct tcttttttaa gttcaagatt atcagaatat tttatattag
     4561 atatttctag agctgagttt acttcatcta aaagatttac ttgtcctgca tcataaactc
     4621 cataagttga catattgatt ttgtatttaa caatctggtt atcactacta tttaaaactt
     4681 ttttatcaac ttcttttttt gcgcttatta atccagaatt tttcttaaag tttatctcat
     4741 catttttatc aatttcacta ttatttgcat caagaacagc tctatttata tagctatctt
     4801 caatactaat tacctttaat gtataagcta taaaaaactt tgattccact tcttctttac
     4861 tgagattaat tgtaaatcca gttataactt tatctacttg cccactttca tttctttttt
     4921 cttctacttt atatgaaata ttattggcaa ttttttcttt actccaagga gtccagctcc
     4981 cattcattgg aatttctaca actctaaaat catgcccagc ttctcctgaa gtagttaatc
     5041 tcatgccttc tggtatatta tctgttataa caacattact tattggtgat ttatcatcat
     5101 ttattgaaat ctgatagttt attacttgac caacttgagt aatatttgtt gctcctgaat
     5161 gtcctttagt aagaagataa tcatctcctc tatcaaatat acttacagta gcatcatctt
     5221 cttgtatctt cttaccatca gattcaatcc tagctacatt cttatattta ttaccatgtt
     5281 ttgcgttttt gtcactgatt ctagccttat acttaacagt atacgattta tctatatttc
     5341 caaaattaac tgaaatagaa tttttactct gttcaatatt aaaatcacta acttcttttc
     5401 catcacttgt tactacactt acagaaccat ttatcaatgt caatccttca ggaagctcat
     5461 catacaaaat aatatctttt aactcttcct gcttttcatt tacttgtata ctgtaattta
     5521 tatctttatt tttaactggc ataaatacag tagttgattt ttttccttca acaagctttt
     5581 taagtgttcc tttagcatct ggaacatccg ttttatctcc aggttttatt tctacatcta
     5641 tatctccact tccactttct atttttccag tagtttcaat atgtattttt gcattagtat
     5701 cttcatcaac ctttttaaca tatgcttcca aacctatttt accttgcatt acactttggc
     5761 cattaggtaa ttctctaaat gtaagtgtat actctccatt actttctgtt ttatcaaagc
     5821 attcacttgt ataatctaaa ttaactttat tgaatacatc tggcacctta aaagttattg
     5881 aatctcctgc ttgtattcct tttgatgttg gagtataagt taaattaaat gatatttttt
     5941 tcattgattc cgtttctatt gtattgttat taatttttac aacaaatcca tagtcttcat
     6001 tttcttcatt aagttcttct tctaaaactt tcaataaatt ttcatcttct aatagattat
     6061 cttcctctgg agttttttct gtatttttat tgtctagtgg tttttcatct tctaaagctt
     6121 gttctgactt tttattatct gttgacttat cttcttctgg tatctgctct ggctttttat
     6181 tatctattgg cttttctcct tctggagttt gctctggctt tttattatct gttgacttat
     6241 cttcttctgg ggtctgttct ggttttttat tatctgttgg tttttcttcc tctggggtct
     6301 gttctggttt tttattatct gttgccttat ctttctctgg agtctgttct ggctttttat
     6361 tgtcgctttc cttctcctct ccagttgatg aattagttga accactttga atctgactat
     6421 tctctcctaa ttctgaagca taagatacag ttggatatat tgtagacacc accatactta
     6481 aaatcataat taaactaatt aataatgcct ttctatttcc tttcttcatg taaatctcac
     6541 ccctttaggt tacctataat ttataatcta atcttccatt aatatcgtat ataaagtaat
     6601 ttttactaaa aaattataca aaaaaagcta tgaaaataaa taaatttata ttcatagccg
     6661 gttgcaccac tgactcagta tatccataag gtgcccaata atagaatata cttcaacatc
     6721 tctataatat ttcataaaaa caattaaaat tgctttattt atattaaaaa tacaattata
     6781 tacttctatt atattatgcc tctatactat tgtctatcct ttttacattt tttaccttat
     6841 gatgagatta attaataatt aaataaaatc cttatctaca acaattacac aattatattc
     6901 aggatttatc tccttaacta attttgttat atcttctttt aagtcatctt ctgacatgtt
     6961 tttggaaaat tcggaaggat taactactat atcaaatatt aaattcttct tgcttccttt
     7021 tccaactatt ctaaaatcat gcatagattt tacaagacta tttttcttaa gtatttcttc
     7081 aagttctttt ttggctgaca taacttcttt atcttcaaga catattgggt ccatgtgaat
     7141 tactagatat atatctaatt tttcagatat ttctctctct gcagtatcta taatttcatg
     7201 tatttccatt atatctatgt tggatggaat ttctgcatgt atagatgcaa tacatctacc
     7261 aactccatag ttatgaataa ttaaatcatg tgtacctatt atatatttat aagataacaa
     7321 tagctctata atagaattta ccatttcttc atctggtgct tcccctaaaa gaggatttat
     7381 agtatctttt acaagagaga aaccagcata aagtatcact aatgatacta cgataccaac
     7441 atatccatct ataggaaaat tagtaaatct agctacaata aatgatataa ctacacagct
     7501 tgaagtaaat acatctccta aagcatcaag tgaaactgct tttaaggctg aagaatcaat
     7561 tttatttccc ataaatttat taaaacgact caaccagatt ttaatcatta tagatgcaat
     7621 taaaagtata agtggtatta cttcaaattt tacaggaatt ggatttacta tcctttctat
     7681 agatgacttt acaaattgaa gtccaaccaa catcaccata aatgctacta ttaatgctga
     7741 taaatactct attcttccat gaccaaaagg gtgttcttta tcagctggct tacttgctaa
     7801 tttaaaacct atcatagtta ttgctgaaga tgccatatca gaaagattat taaatgcatc
     7861 agccattata gctatactag atgtgagcat tcctatgaat acctttatca caaataaaag
     7921 taaattggat aatataccta ctacaccagc tacataacca tacttatttc taacatctgt
     7981 attttgaacg ttttcgctat ctctgataaa tgttttgact aggattttgg aaaacatgaa
     8041 acttacctcc ctgcccttta ttttattaac aaattttata atctatatct ttatttatga
     8101 taacataatt tatatataaa attcttaaaa atcactctac ttcagtaaaa tattattcta
     8161 tatatctatt tagatacgaa ttttatattt agtaaagttt ttatggctta gtgtctgttt
     8221 aatagattct atataagttt ttacaaagat tatttattat gataatatca tttttcccaa
     8281 gtattactta gaatatgatt tttttgtttt gtctgtacat attttaaatt tatactatat
     8341 ttatctatat tttataacct tatgatacac tataactata agtttttaaa tggaaaggaa
     8401 tgaaataaat catgtttttt atcagttata tttacacaaa atcacctatt gaatttgata
     8461 ctcctttgca aaaagaggca tacaaaatat tacaaaaatt agatattgac tttgaatgtg
     8521 ttgacacaga tgaagcaatt acaatggaag attgtgttta gattaataaa aaattaaata
     8581 tgaaaatggt aatataaaac aacattaaac aaacaaaaac cgcaaagtta caatatatag
     8641 tactttgcgg tttcatagga attaattttt actattaaag ttcatagttc tactattttt
     8701 cattgttcta agaattagct ttacaaattc atttgacaat aggatagtgg atgcaaatag
     8761 tattatcttt atccacatat caaagtccaa agaaacagaa ttaaagaaac tctggaacac
     8821 ttgtgtaaat attatttgaa ttatccctgt aactactatt atttgcaatg ccagtttatt
     8881 tttaaagaaa tttggtattg tactatttaa accaaactct ctacaattta atgcattaaa
     8941 aagggcactg aatgcaaata aagaaaatat aacagtacct tgttcttctg atgtagctcc
     9001 caatatatta aaagctgatt gagtaaatac aattgtaatt ataagaatag catttataat
     9061 aatatttaca aacatagacc tagctattat tccagaatgt ctatttattg gttttctttt
     9121 caatacataa tctctaacag gttctaatcc taatgctagg gctggaggac catccattat
     9181 tatatttacc cataataatt gtattgtagt aaatggcata tccttaccag taagttgaga
     9241 tattatagca attataaatg ctactatatt tacagtaagc tgaaactgta taaatctttg
     9301 aaagttctca tatatacctc ttccccattt tattccctct actatagtac taaagctatc
     9361 atcagtcaat attatatctg ctgcattttt agatacttct gttcctgaaa tacccattgc
     9421 aattccaaca tctgcttgac taagtgctgg ggcatcattt ataccatcac ctgttacagc
     9481 tacaacttcg ccactttttt gaagtgctga aactattctc atttttgtat ctggtttact
     9541 tcttgcaaca atagatattc ctttgatttc ttctctcaat tctttatctg tcaatacatc
     9601 tatatatgtt gcttcaactg cctttttccc atcatttaat aatcctaatt catttccaat
     9661 agctgttgct gtatttatat tatctcctgt aagcattttt acatctactc ctgcattaaa
     9721 tgctttatca atggaatctt tgaccccttc tctcaatggg tccactattc ctacaaatcc
     9781 actaaaaact aaattatctt ccttcatata acttcttgaa tttcctatta aattcaattc
     9841 accttctgaa gtcattgcaa cttctgtttt agaattactc atttttttat aagcaaatcc
     9901 taatgttctc atagatttaa tttgtaactt cttaatctca tctaaaatac ttttttctac
     9961 ttttggagtt attggaacaa tattttttcc ttgttgtaca taagaacatt tttttaataa
    10021 tacttctgga gcaccttttg aaagtaatat atctgaatcc tcttgacgaa ttaatgttga
    10081 catcttcttt tcttctgagc taaatggtat ttgtgatatc aaatacgttt gttttctcat
    10141 ttcattatag ttcttatcat tatgatataa aagtaatgca cactctgttg cgcttcctat
    10201 atatttaaat gaattatctt ctttttctat atctgcagtt gaatttacta tacagttttg
    10261 ttcaaaatag ctattacttt gatactctcc tccacttata tatttaccat caacatatgc
    10321 tacctctacc atcattttgt tttgtgttaa agtccctgtt ttgtcggaac atattacaga
    10381 aacagaacct atagtttcac aggcttcttt ctttgttact aatgcattta tctttgccat
    10441 cttttgcatt gttatagcta aggtaatatt tatcatagtt ggcaaacctt ctggaacagt
    10501 tgcaactatt aatgcaatac aaaccatata tgcattttta gcaggttcta atgattgtaa
    10561 gaaagataat acactgcttg tatctaaaat taacatacct tttctctgca tctgtataat
    10621 cataaacata cataataaag tagctattac gcctgatatt cccgctattt tagcacccaa
    10681 cttccctaat ttaatttgaa gtggagtttg ctggtcttca tttgataaat tctgtgctat
    10741 cttacccatc tctgtcttat caccagtatg agtgacaacc attatgcccc taccatatgc
    10801 tacaagagtc cctccaaata ccatattgac ttgctttgca ggtattgcat cctgttcgat
    10861 tatttctgtt ttagaataaa ctacttccat atctaataca gcatcagcat tcttagctac
    10921 atcatcagat tctcctgtta gcatatcttc tctaagtttc aaatttatag actgtataag
    10981 tcttccatct gctggtatca tatccccagt ttctatatat acaatatctc ctggtactaa
    11041 atcatttttt gatattttaa ttatcttccc atttctaagc acctttactt ctatattttc
    11101 tgttaatttt gacagggcat gagctgcttt tttagatttt ccttcagtta ttatgcctat
    11161 agaaattcca ataaaaatag cccctaaaat acctaatgcg tcatgtaatt ctcccacaaa
    11221 agaacttatt actgctgctc ctatgagaat taatatcata ggttctgtca aactttctcc
    11281 caactcgtcc caaaatgttc taccttcttt tatagtaaat tcatttaatc catatctaag
    11341 cttcctttct tctacctcat tatcatcaag tccaatttct ggatttgttt ttaagtattt
    11401 taatacttct ttggtaggtt tattataata cctcataact ttatccccct tgttaattat
    11461 ctataaggaa ttatatgctt atgatagctt gtatattact tttcaaaaaa aataaggtgt
    11521 tgattaatca acaccttata gtggttatat attattaaat tttatgttac aggctttata
    11581 aattgtgagc tatacaaatc aaaatattta atcttcaaaa tacccttctg gatataatgg
    11641 aaacttagat ataagttctt gtgcctctgc tttacattca tctaatactt ctttattatc
    11701 aatattttct gctaccttat tcattatccc tgctatctgt ataacttctt tttctttcag
    11761 acctctttga gatattgatg taagacctat acgaacacca ctagttacaa atggtgattg
    11821 tgggtcgtca ggtataacat ttttattaac tgttattcct acatattcaa gagctttttc
    11881 aaattgtttt cctgtaagat tcttacttct taagtcaacc attactatat gattatcagt
    11941 acctccactt acaatactaa aaccatgtga aatcagttca gaagctagag tctgagcatt
    12001 tactacaact tgctccatta tctttttaaa tttatctgta cttaaatata atagtgaaaa
    12061 ggcttttgct gcaactgtat taagatgtat agaccctaat gttcctggaa aaacaccttt
    12121 atcgagtttc tttgcatgtt ctgctttaca aagtacaata ccacttcttg gtccacagaa
    12181 agtctttgtc gtagatgaag aaacaaaatc tgcataaggt acaggactag gtattacttt
    12241 cgctgcgact aatcctgcaa catgtgccat atctaccata aagtaagctc ctacttcttt
    12301 tgcaaccttt gatattcttt cataatctat taatcttgga tatgaacttg cccctgcaat
    12361 tataagtttt ggtctacatt cttttgcctt tgcttcaaga gcatcataat ctatacgtcc
    12421 agtatttggg tctaccccat aaaattcata tttataaatg tcatgtaaaa aattaactgc
    12481 gctaccatgt gtcaagtgtc caccttggtc aagacgcata cttaaaacag tgtcatttgg
    12541 ttttaatatt gatgaatata cacaatagtt agctgtagaa cctgaatacg gttgtacgtt
    12601 tacatgttct gctccaaata cttcttttgc acgttttatt gctagggttt caagtttatc
    12661 tgcttcttca gaacctgctt gaaaacgagc ccctggatag ccttcttctg ttttatttgt
    12721 aaatacacat ccacttaatt caagtacttc tgttggtgct gtactttcag aagctatcat
    12781 ctcaatattg tgttcttgtc taaccaactc atcagctaca atcttgtata actctgggtc
    12841 tgaaattttt gttgttttta acataataat cctcctctta attaaagaat atattttatt
    12901 agtttaatct tcattgataa atttattata acggtctata aaacgtaaga tattcctcca
    12961 ccaggtccta atggtatgcc aatacccatg aatagtaaca atattattat ttctattaat
    13021 gtaaatacca ctgagtatgg aatcatattc gatatgacag tacctatacc tatatcttta
    13081 tcatatcttc tggcaaaggc caatataact ggaaaatacg gaaaaagtgg acttattgga
    13141 tttgttgaag catctccaat acgataagct atttgagtta atgctggatt aaagtctagt
    13201 agcataaaca tgggaacaaa tataggtgct aaaatcgccc attttgcaga agcacttcct
    13261 acaaataaat tgataaatcc agatagcaat ataaatccta ttatcaatcc atagctaggt
    13321 attcccgact ctgctagtaa ttttgctcct gttattgata aaattgttcc taagttacta
    13381 agattaaata aattaataaa ctgggatgct ataaatgcta ggactatata tccacccata
    13441 tcactcatac tactacccat catagatact aaatctttgt cactttttat cttcttactt
    13501 acttttccat acactaatcc tggagttaaa aatataatag tgattatagg aactatacct
    13561 ttcattaaag gtgcattact agatagtata gaaccagtct ctggatcttt cataaatgca
    13621 ttgtcaccta tacataaggc aactatcact actactacaa ctaacaatga taataaagcg
    13681 tattttatac ccttattttc aagttcactc aattcctgta tatccttatc atttgttgaa
    13741 tcattatatt ttgtaaaacg aggagctata aatttttcag ttacaaatgc tcctgcaaaa
    13801 gttaaaacta tcgctgatat aaaagcaaaa taaagattca ttgctggatt gccttggtaa
    13861 gatgggtcca tcatttgagc tgctggtatt gtaaatccag ctaacaatac atctgtagta
    13921 ctaagcataa catttgctgc aaatccccct gcaacacctg caaaagctgc aaacatacct
    13981 acaagaggat gtcttccaat tcctaaaaac acaagtgctg caagtggtgg taatattata
    14041 aatcctacat ctgctgctgt atgtgacaac atacctgcaa atacgattgc tacagttaca
    14101 agtttttgtg gaactttact tatacccttt ttcattaaca cttccataaa tccagatttc
    14161 tcagcaacac ctgctccaag cattgtaacc agtactaatc ctaaaggagc aaatgactga
    14221 aagtttgtaa ctatgctacc taaatatgat tgaatctgct ctttctctag tagatttact
    14281 acctttacaa cttcttttgt agaaggatgt atttcttcta ctcctaaatt agatataact
    14341 gctgacaata ttacaacaat tatacatagt aataaaaata tggttactgg gtctggaagt
    14401 ttatttcctg ctctctctac aacatttagt gccctcatca ataatccact atctttgttt
    14461 ttttcctgtg aattaatttt cctcaaacta ataccccctc ataaacttta tctccagaat
    14521 gctctatctt aaattttatt ttttaattta tccatataat ttataacaga ctgacatagt
    14581 acatttgtac ctaccaatat agaattttta tcagctttaa atttaggatt atgccactga
    14641 tatatacaat cttgttcttt gttgcctacc cctaaccagt aaaaaaatcc tggggctttt
    14701 tccatgaata tagaaaaatc ttcccctcct gtagaaggta ttggatcaac tatagcatct
    14761 tctgaataca aatcacacac agacttacaa gctattgcgt acatctcttt actatttata
    14821 acagctggta aatcaaataa ataatctagc tttgcagttg cttgatatcc acgagctatc
    14881 tcacaaacta tattttctaa attttctgat attttctttc gtacttcatt actaaagctt
    14941 ctacatgtgc ctgtcatatc taccttttct gaaattacat tatttgcagt ccctccattt
    15001 atagtaccta ttgatataac agctgattct aatggactta tgtttctgct tactatggtt
    15061 tgaataccca ttataattgc acttgatgct actatagggt ctattgttct gtttggtata
    15121 cctccgtgac ctccaaatcc atttacttct atcttaatag tgtctactgc tgccattaac
    15181 ccacctaact taacacctat ttttccacaa ggtatatctg gatgattatg aagtccaaat
    15241 atagcatcaa cttttggatt ttccattact cctcgttcca ctaacatctt cgcaccttta
    15301 ttgacttctt cggctggttg aaatataaac tttacatttc catgaagact atctctaata
    15361 tgagacaata catatgcaca tcctacaagg caagacgtgt gtatatcatg cccacatgca
    15421 tgcattatac catcatatct ggatttatac ttaatatcaa cctcttcata tattggcaac
    15481 gcatcaatat ctcctcttag tgctactgtg ggaccatcat acttcccttt taataatcca
    15541 acaacgcctg tttccattcc taaatctaat atttctatgt ctaaactcac taataaatct
    15601 tttatagctt ttgtagtcct aaattcttta aaacttaatt cggggtgcat atgaatatct
    15661 gtaaaaaaat ctaaaatatc ttctttatac ttttctgaga ttaaaataag ttcatgcata
    15721 ctaacacctc tccaaaattt tattttttta tttttta
//
LOCUS       DAMJWS010000094        15131 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMJWS010000094 DAMJWS010000000
VERSION     DAMJWS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.098"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            82..252
                     /locus_tag="PC399_003128"
     CDS             82..252
                     /locus_tag="PC399_003128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593972.1"
                     /translation="MNNDKPFIEFEEEVDFILTECKKEGVSIDRDTIELILDLDLKFL
                     ELKGIATPLEDE"
     gene            complement(311..3292)
                     /locus_tag="PC399_003129"
     CDS             complement(311..3292)
                     /locus_tag="PC399_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDF2593973.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVDDLFRFL
                     ENTQKELLDEFKYNRGSNYKKAFIDLLNSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKKDRNPKETLFGFNQRVLVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     RGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKGNQRAIFPRFHQLDAV
                     RKIVEDLKINKVGKNYLIQHSAGSGKTNTIAWTSHRLSSLHDEENNAIFDSIIVVTDR
                     KVLDKQLQNAIYQLEHKNGLVVKIDEDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLDKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKVIDAENEKNEKSSIDKMN
                     ELIEKRTNLGNISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPISVYYKVGKKIIDNPEFDKGEAKRAINKFVSLNEHNIRQKVETIVDDFVDNR
                     SMWLEGKAKGMIVTASRLHAVRYKLAIDQYIKEKGYDMKALVAFSGTVKDGDDEYTEV
                     GMNKEVAPDITDSNLPNIFDKNEFKLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKQTFILDFQNSVEDIQDAYKPYFEMTNIDKVTDPNVVNDLWYQLHEY
                     AVYTDEEVNDFTVLFYKEKRTSSQDAKMNNLIDKAVERYCYLEEDDEKKSDEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLKKLPKKSKQRIDLDDMLSLDYYKLQKL
                     GLDDKKGTDISLYNGEGELVGISETATSRVSEADEEYLEDIIKKINDVFGVGLTEDDR
                     IMINTYEEMFKNDKDIMDIAKANSYEDLVSTFGKKYFKRGIVKTKNRNDRLVKEILTN
                     NNLQNYMIHYLAEKIYEEANR"
     gene            complement(3327..4598)
                     /locus_tag="PC399_003130"
     CDS             complement(3327..4598)
                     /locus_tag="PC399_003130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593974.1"
                     /translation="MDKNIFQININTDVLIRLRNQINEEYYISYRKEFYDKNNKKHRA
                     WDKICAIMDRLDDTVDYLNKLKLNTGKYRRSAFDFFEFMNQASVVMDCITHLAKIFEV
                     PYEEVETSTEIFNELGKEGKGTDKKYFEYLRSLCSVHPMETSRHKIYQENSFECSPYV
                     VWNDKSIWTNDASDLYAVVYTSKDNDSFKRIHIYMNQIFEYVKRRVNFIENIIEHIEK
                     YQQEIIEIFRNTPIKKEEDFESYIDYLRNLKKEIEIRYGNDSFHELNYLIELFDLPLS
                     NPDNEEKMNLYKNALKYAIKFEHNSIQNMTNEGYKNNGILYPQVNITTTLLEELISLN
                     SGSEERRKYRYAIEKTSYLSYDSCWSDREWAYDQIDDMMPFLERYVTFDKAQSDFEHY
                     VLVKLALYLECLENECLINKNVPNDKKFRIK"
     gene            complement(4660..6051)
                     /locus_tag="PC399_003131"
     CDS             complement(4660..6051)
                     /locus_tag="PC399_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2593975.1"
                     /translation="MGYRYRSDDELKDSGVEWLGKIPKDWEVRQLKRVLSDRKENNEP
                     IKTSNILSLTIEKGVIPYSEKGTGGNKAKEDLTQYKLVYPNDIVLNSMNVIVGAVGLS
                     KYFGCASPAYYMLYKINANDDIRFYNYIFKSKLFQDSLKGLGNGIMMKQSETSGKLNT
                     IRMRIPMEKLSSVKIPIAFNLDQEKIANFLDEKTSQFDSIISKKEKLIERLEEAKKSL
                     ISEVVTGKVKVVKTDDGYELIKRSSEEMKDSGVEWLGEIPKEWDIKRLRFLGKLQNGI
                     SKSSDSFGYGYPFVSYSNVYRNIELPHIVEGLVNSTEEERKIYSVEEGDVFFTRTSET
                     VEEIGFASTCMKTIDNSTFAGFLIRFRPNDKRLYKGFSKYYFRCDLNRKYFVKEMNLV
                     TRASLSQNLLNNLSVVLPKYEEQVEIYNALELKVSYINLSINKLKYQIEKLKEAKQSL
                     ISEAVTGKIEILD"
     gene            complement(6051..8078)
                     /locus_tag="PC399_003132"
     CDS             complement(6051..8078)
                     /locus_tag="PC399_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2593976.1"
                     /translation="MSIDNNFNHVAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIIKSVYEEYDFLPEEERDELVIVDLKENHDINLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNNVKEIIGKFKFKDEINQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNKMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEILFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKINPSAIVNVYGQELLDESFAICQADMLMKGQNPDNIRLGNT
                     LTQDRFNGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIENDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEAKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEQIKEI
                     YEKFEESENSKIFDNEDFGYRKVTIERPLKLSFKVNEEAIENVKNTTQFINLAVSKKK
                     DIEVKEKEEAEGKIKQDKLIKLLESFDNTLEYMDRDKFIKDLKAKSKEFDIQLSAGLI
                     KAIVNTIGVRNEDAEVCKDSKGNIESDSSLKDTESIGLKDDVYEYFEKEVKPHVNDAY
                     IDESSINNIGYEIPFTRHFYKYEELRSFAEIMKEVESLESEIALKIRKVLG"
     gene            complement(8193..8542)
                     /gene="ssrA"
                     /locus_tag="PC399_003133"
     tmRNA           complement(8193..8542)
                     /gene="ssrA"
                     /locus_tag="PC399_003133"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8700..9155)
                     /gene="smpB"
                     /locus_tag="PC399_003134"
     CDS             complement(8700..9155)
                     /gene="smpB"
                     /locus_tag="PC399_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF2593977.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            9733..10560
                     /locus_tag="PC399_003135"
     CDS             9733..10560
                     /locus_tag="PC399_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2593978.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            11560..12023
                     /locus_tag="PC399_003136"
                     /pseudo
     CDS             11560..12023
                     /locus_tag="PC399_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(12290..12934)
                     /locus_tag="PC399_003137"
     CDS             complement(12290..12934)
                     /locus_tag="PC399_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593979.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(12864..14153)
                     /locus_tag="PC399_003138"
     CDS             complement(12864..14153)
                     /locus_tag="PC399_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2593980.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(14150..15022)
                     /locus_tag="PC399_003139"
     CDS             complement(14150..15022)
                     /locus_tag="PC399_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2593981.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         4901 a         2485 c         1655 g         6090 t
ORIGIN      
        1 ttttacaaat aatgttattt ttttctcttc tttgatataa tttaaagtaa atacattata
       61 tttaatcaaa ggagcaaaaa tatgaataac gataaaccat ttatagagtt tgaagaagaa
      121 gttgatttta tacttactga gtgtaaaaaa gaaggtgttt ctatagatag agatactata
      181 gagcttattc ttgatttaga cttaaagttt ttagaattaa aaggaattgc tacaccttta
      241 gaagatgaat aatatacaat agctcataat aaagtaccta tggagagtta attagctctc
      301 tataggtatc ttatctattt gcctcttcat atatcttttc agctaaataa tgaatcatat
      361 aattttgtaa attattatta gttaatatct ccttaactaa tctatcattt ctatttttgg
      421 ttttaactat acctctctta aaatacttct taccaaaagt gctaactaaa tcttcataac
      481 tattagcttt agctatatcc atgatatcct tgtcattctt aaacatctct tcataagtat
      541 ttatcataat tctatcatct tccgtaagtc ctacaccaaa aacatcattt atttttttaa
      601 ttatatcctc taaatactct tcatcagctt cacttacacg agaagtcgca gtttcagata
      661 taccaactaa ctcaccttct ccattatata aggatatatc cgttcctttt ttatcatcta
      721 atccaagctt ttgcaattta taataatcaa ggcttaacat atcatcaagg tctattcttt
      781 gtttactctt tttaggtaac ttctttaaaa gtgctactaa ataaacttgc aatcttaaaa
      841 gatttaagtt ctttatagga taaatctgaa ttaaaaaatt ataaaaagta atatactttt
      901 gacatttttt cttaaactca tcagattttt tttcatcatc ttcttctaaa taacaatacc
      961 tttcaactgc cttatcaatt aaattattca tcttagcatc ttgagaagaa gttcttttct
     1021 ctttatagaa taatactgta aaatcattta cttcctcatc agtatatact gcatattcat
     1081 gtaactgata ccaaaggtca tttactacat ttggatctgt taccttatca atattagtca
     1141 tttcaaaata tggcttatat gcatcttgta tgtcttccac actattttgg aaatctaata
     1201 taaatgtttg tttattagga taagttctat taagccttga taaagtctga acagttttaa
     1261 caccatctag ttttttatcc acatacatag cacaaagttt tggttggtca aatccagttt
     1321 gatacttttc tgcaactatt aatagcttaa attcattttt atcaaatata ttaggtaaat
     1381 ttgaatcagt aatatctggt gctacttcct tattcatacc tacctctgta tactcatcat
     1441 caccatcttt tacagtacca gaaaaagcaa caagtgcttt catatcatat cctttttctt
     1501 ttatatattg gtctatagct agtttatatc taacagcatg taatctactt gcagtaacta
     1561 tcataccttt agctttacct tctaaccaca tagacctatt atctacaaaa tcatctacta
     1621 tagtttcaac cttttgcctt atattgtgct catttaaact aacaaactta tttatagccc
     1681 tcttagcctc tcccttatca aactcagggt tatcaataat tttctttcct actttgtaat
     1741 aaacacttat aggcataaag ttttttagta catctaatat aaatccttcc tctatcgctt
     1801 gtttcataga atataaatga aattcatgag gtagtccatc ttcacctatc ctcccaaata
     1861 cttgcatagt cttattctta ggagtagctg taaaagcaaa gaaacttata tttcctaaat
     1921 ttgttctttt ctctataagc tcattcatct tatctatact agacttttca ttcttttcat
     1981 tttccgcatc aattacctta gcttcttcta aagtctttcc tgctaaagtt tcttttaatg
     2041 cattcatatt ttcaccagat gttgaagaat gtgcttcatc tataattact gcataacgcc
     2101 ttttatcaag ttgttctatc ttaggtagtg catactggaa tttttgaata gtagttataa
     2161 taatctttgc tctatttaca atagcatctg ctaagtctga tgaattttta tcttcatcta
     2221 tcttaacaac aagtccgttt ttgtgctcta attgatatat agcattttgt aattgtttat
     2281 caagtacctt tctgtctgta actacaatta tagagtcaaa tatagcatta ttttcttcat
     2341 catgaagact tgataaccta tgtgaagtcc atgctatagt gttagtctta ccactaccag
     2401 cactatgttg gataagataa tttttaccta ctttatttat ctttaaatct tctactatct
     2461 tccttactgc atctaattga tgaaatcttg gaaatattgc tctttgatta cctttatcat
     2521 cttcttgaat aaagtaaaat ctttttataa tatcaagtaa actatcttta gtaagtattt
     2581 cttcccaaag ataagaagtt ttttcttttc cttctactac aggatttccc ttacctctat
     2641 tgttaccttt attaaacggt aagaagaatg ttttatctcc ttttagctct gttgtcatat
     2701 aaacttcatc agtatctact gcaaaatata ctaacactct ttgattaaat ccaaataaag
     2761 tttcttttgg gtttctatct tttttgaatt gctttatagc attatacaca ttttgattag
     2821 taaactgatt tttaagttcc attactataa cgggaaatcc atttaaagat aatactatat
     2881 ctaaagtatt gttgtgcttt gagctatatt ttaattgtct tgttattttg aatatatttt
     2941 gtttataatt ttcatacgct gttttattag tagttagatt tggtttgttg tagaataatt
     3001 gaaattttcc gttcatcatt atatcttcta caccatttct aagtacactt ataagacctc
     3061 ttcttttgat gtttgaattt aataaatcta taaaagcttt tttgtaatta cttcccctgt
     3121 tgtatttaaa ctcatctagt aattcttttt gagtattttc taagaatcta aataaatcat
     3181 ctacaaataa gcaattttca aggtcaaatt cttgtgaatg accttgttca tatccaattg
     3241 aaactaaatg attttcaata gattcttcta ggtgtttttc ttttggtaac ataatatcct
     3301 ccctctccct attttattag ctataattat ttaatacgaa actttttatc attaggaacg
     3361 ttcttattta ttaaacattc attttctaag cactctaaat aaagtgctag ttttaccaat
     3421 acataatgct caaaatcact ttgagcttta tcaaatgtta cgtatctttc taaaaaaggc
     3481 atcatatcat ctatttgatc atatgcccat tctctatcac tccaacaact atcatagctt
     3541 agatatgatg ttttttctat tgcatatctg tacttacgtc tctcctcact accactattt
     3601 aatgaaatta gctcttctag taatgtagtt gttatattca cttgtggata taatattcca
     3661 ttattcttat aaccttcatt tgtcatattt tgaatactat tatgctcaaa tttgatagca
     3721 tattttaaag catttttata taagttcatc ttctcttcat tatcaggatt tgataatggc
     3781 aaatcaaata attctataag gtagtttaat tcatgaaaag aatcattacc atatcttatt
     3841 tctatctctt tttttagatt tcttaaataa tctatataac tttcaaaatc ttcttctttc
     3901 tttattggag tattcctaaa aatttcaatt atttcttgtt gatacttttc tatatgttct
     3961 attatatttt caataaaatt tactcttctt tttacatact caaatatttg attcatatat
     4021 atatgaattc ttttgaaact atcattatct ttacttgtgt ataccactgc atataaatcg
     4081 ctagcatcat tagtccatat acttttatca ttccaaacta cataaggact acattcaaaa
     4141 ctattttctt ggtatatttt gtgtctacta gtttccattg gatggactga acacaatgaa
     4201 cgtagatatt caaagtattt tttatctgta cctttaccct cttttccaag ctcattaaaa
     4261 atttcagttg aagtttcaac ttcttcatat ggtacttcaa atatttttgc aagatgtgtt
     4321 atacaatcca ttactacgga agcctgattc ataaattcaa agaagtcaaa tgcacttcgt
     4381 ctatatttac ctgtatttaa tttgagttta tttaaataat ctacagtatc gtcaagccta
     4441 tccattattg cacatatttt atcccaagcg cgatgttttt tattattttt atcataaaat
     4501 tcttttctat aagatatata atattcttca tttatttgat tccttaatct tatcagtaca
     4561 tctgtattta tattaatttg aaatatattt ttatccatta attctccctt ttatttaact
     4621 ttgtatgtat aatctatatc tatattaaga tactagaagt taatctagta tctctatttt
     4681 tcctgttact gcttcactta ttaaactttg tttagcttct tttagttttt ctatttggta
     4741 ctttaactta ttaatcgata aattaatgta gctaactttt aattcaagtg cattatatat
     4801 ctcaacttgt tcttcatact taggcaatac aacagaaaga ttatttaaaa gattttgaga
     4861 taaagatgct ctagttacta aattcatctc ttttacaaaa tattttctat ttaaatcaca
     4921 tctaaagtaa tatttagaaa atcctttgta tagtctttta tcattcggtc taaaccttat
     4981 taaaaatcct gcaaaagtag aattatcaat agttttcata cacgtagaag caaaaccaat
     5041 ttcttctact gtttctgaag ttcttgtaaa gaatacatct ccttcttcta ctgaataaat
     5101 tttacgttct tcttcagttg aatttactag accttctact atatgtggta attctatatt
     5161 tctatataca ttactataac taacaaatgg ataaccataa ccgaatgaat cactactttt
     5221 acttataccg ttttgtagtt ttcctaaaaa tctaagtctt tttatatccc attccttagg
     5281 tatctcacct aaccactcaa ctccactatc cttcatctct tcactacttc tctttataag
     5341 ctcataccca tcatcagtct taacaacctt aaccttacca gtaacaacct cacttataag
     5401 gcttttcttt gcttcttcta atctttctat taacttttct ttcttagata taattgaatc
     5461 aaactgtgaa gtcttttcat caagaaagtt tgctattttt tcttgatcta agttaaaagc
     5521 aattggtatc tttactgaac ttagtttttc cattggaatt ctcattctga tcgtatttaa
     5581 cttacctgaa gtttctgatt gtttcatcat tattccatta cctaatcctt ttaaactatc
     5641 ctggaataat ttagatttaa aaatataatt ataaaatctt atatcatcgt tagcatttat
     5701 tttatataac atataatatg caggactcgc acacccaaaa tattttgata gtcctactgc
     5761 accaacaatt acattcatac tatttaatac aatatcattt ggatatacta atttgtattg
     5821 tgttaaatct tctttagctt tattaccacc agtacctttt tcagaatatg gaataactcc
     5881 cttttctatt gtcaatgaca gtatattact tgtttttata ggttcattat tttcttttct
     5941 atctgatagt acccttttta attgtctgac ttcccaatcc ttaggaatct tccctaacca
     6001 ctcaactcca ctatccttta actcatcatc acttctatat ctgtatccca ttaccctagc
     6061 accttcctta tctttagtgc tatctcactt tctaaacttt ctacttcctt cataatctca
     6121 gcaaaacttc ttaactcttc atacttataa aaatgtctag taaatggtat ctcatatcct
     6181 atattattta tactactttc atctatataa gcatcattaa catgaggctt tacttccttt
     6241 tcaaaatact catatacatc atctttaaga cctatacttt cagtatcttt taatgaacta
     6301 tcactttcta tattaccttt gctatcttta caaacttctg catcttcatt tcttacacca
     6361 atagtattaa ctatagcctt tataagtcct gctgaaagtt gtatatcaaa ctcttttgat
     6421 ttagctttta aatcttttat aaatttatcc ctgtccatat actctaaagt attgtcaaag
     6481 ctttctaaaa gttttattaa cttatcttgt tttatttttc cttcagcttc ttctttttct
     6541 ttaacttcta tatctttttt cttagaaact gcaagattta taaattgagt agtatttttt
     6601 acattttcta tagcttcttc gtttacttta aagcttagtt ttaaaggtct ttctattgtt
     6661 acttttctat atccaaaatc ttcgttatca aatattttac tattttcact ttcttcaaat
     6721 ttttcatata tttcttttat ttgttctatt tgttcaagac ttatttcttt tcttttgttt
     6781 cctaagcttt ttctcattaa ttgataatat tcactagcat ttacaagttg tatcttacct
     6841 tttcttttag cttctttttt gttagtaagt acccaaatat atgtagctat tcctgtattg
     6901 tagaacatat cagtaggtag tgctattata ccttctagta aatcattttc tattatccat
     6961 tttctaatgt ttgattctcc acttcctgca tctcctgtaa ataatggact tccgttaaat
     7021 attatagcta ttctacttcc ttcttcatca tcatacattt tacttatcat attttgtagg
     7081 aaaagtaatg aaccatcact tactcttgga gtacctgctc caaatcttcc atcaaaacct
     7141 aagtctgctt cttcttttac tttcttttct tcgtctttcc aagttacacc aaaaggcgga
     7201 tttgatatta aaaatcttat tttttctccg ttaaatctat cttgtgttaa agtatttcca
     7261 agtcttatgt tatctggatt ttgccctttc attagcatat ctgcctgaca tattgcgaat
     7321 gattcatcaa gtagttcttg tccatataca tttactattg cacttggatt tattttttct
     7381 acatagtctt cagctattga aagcattcct ccagtaccac aacaaaagtc tgctatagat
     7441 attacttttc cttcttctgt aaggaagttt tctttaccca taaatagtat ttccatacaa
     7501 agtcttataa cttctcttgg tgtgtattgc tctcctgctg ctgagttttc tgtaaatctt
     7561 cttaacattt cttcgtatat gtatcccatt ttattgttag atactgagtt tatatgtaga
     7621 tctacttcat acattttgct aagaactgca tatagcttgt cttttttatc taattgattt
     7681 atttcatctt taaatttaaa tttacctatt atttctttta cattgtttga gaatccgttt
     7741 agatattctt caaagtttga ttttatattt tcactatcat ctaatagttt tttccatgta
     7801 aaatcactta cattgtaaaa ttgtaagttt atgtcgtgat tttcttttaa gtctactatt
     7861 actagctcat ctctttcttc ttctggtaag aagtcgtatt cttcgtatac tgattttatt
     7921 atttctctat cataatcatc taatagacaa tcaaatcttc ttattactat tagtggtaac
     7981 attacttttc tatagtcttc ttctttatat gtacctctta aactttctgc tatattccac
     8041 agaaatgcaa catggttaaa attattatct atactcactt atactatctc cttttagtta
     8101 aattttacat tagtttatat tataattata tcatttcaag gcacaaaaaa aacatattga
     8161 ctaaactatc aatatgtttt cgtggatttc aatggtggag acgaggggag tcgaacccct
     8221 gtccgcaaat cttttccttg ctgcatctac atgcttagcc aacttcacta atatctcacc
     8281 tcacatagcg ccaagtggca ggctctatgc ttagccagtc ccaattaatc tacttcaagt
     8341 cgagacaccc ctaaagtagt tccctgcata atgacgtcca gtcttagtcg gcaggaggac
     8401 taagagggac gagcagcaac taggctgcta tcgcgaaatt atcgtctgcg tttattttaa
     8461 agttccccag tttttacgtg acccagagta acacgacatg cagcctcaag ttccaaaccc
     8521 acgtcgaaac ctttacgccc ccttgagtag tttatagtcc agtagacttt tctcaaactt
     8581 tatcaccatt tttattaaat ttttaatgta tatataatat aatactttta agaataaaaa
     8641 tcaatgttat gttctagata ataaagaatt ttattctaaa aaatacagtt ttatctagtt
     8701 taatacttac ctgtcatttc tctttctata cgtctttgtg catctttctt agccatatct
     8761 tgacgtttat catatagttt tttacctttt gctaaggcaa tctctacttt aactttaccg
     8821 ttttttagat acaaactaag tggtataagt gaatacccct ttatagtagt tgctccaata
     8881 agctttctta tttcatgctt atgaagtaat agttttctaa ctcttaaaga atcaacattg
     8941 aatatatttc cttgctcata tggacttata tgaacttgtt ttaaaaatac ttcgctatta
     9001 tctacagatg caaatccatc acttaggtta actttgcctt gtcttattga ttttacttct
     9061 gttcctttta attcaattcc acattcataa gtttcttcta taaaatattc atgtcttgct
     9121 cttttgtttg ttgctaatat ttttttacca gccatataaa tctcaccttc tttgctagaa
     9181 atttgttcca taaatgttag aaatttacta aatatttaat gttgccaaat tatttgacta
     9241 acttgtatga taacatattt aggcatttta gtcaatattt cactgatata ttaagttagt
     9301 taggctatat ataattaagt aatataaatg tatgaaaata gctaaaaaac tacttcgaat
     9361 atacttaata ctaaactaac tctaaccagt aatactaatt tataaatttc aaaacaaaaa
     9421 tattacatat tataaatctt ctatcttcta tcttctatct tctatcttct atcttctatc
     9481 ttctatcttc taaataattt taacatgccc ataaaatata atacccacaa atgataaata
     9541 atactcaaaa tgtctattga aataactata catccttaaa taacttaact taaatctaaa
     9601 catttcaaaa acttcttaaa aatatgtaaa aatttgatga taaagtagcg attttttcaa
     9661 aaaaaatgta atatagagtt atatcttata atattttaga gataaatttt atcatagtgg
     9721 ggagaaattt gtatggattt aagcaaaaaa gtaaaaattt tagaaaatgc atcagatggc
     9781 aaaagcgaat ttgagatatt agaatttgaa aaacttgagg gagcttctag cccattaatg
     9841 gcaatgggat tatattttgc tagagaagct ggtttgaaag ctagacaaat taagataaaa
     9901 ttaaataatt catcaataaa aacagaagct ggtgcattat actattataa aggaaatata
     9961 tcttctgaaa ctaagattgg tggtgttgga ggtcttttta aaaaaggaat atcaggagca
    10021 cttactaatg aatcaatgat gaaacctgta tacaaaggta ctggagaagt ttatttggaa
    10081 ccttctttta gacattattt atttatgagt cttgataatg attctataat tgttgataaa
    10141 ggattattct attgttgctc tgatacaata gaagtaaaag catttacaca aaagaacttc
    10201 tcatctgctt tacttggtgg tgaaggcttc ttccaaatag aattaagtgg tactggtgta
    10261 atcgttctgg aatgtgttgt acctcaaagt gaaattgtag agtatgaatt gaaaaatggt
    10321 gaagaattaa aggtagatgg taactttgca atagcaagaa catctggtgt taatttttct
    10381 gttacaaaat cagataaatc tttaattggt tctgctttaa atggtgaagg tttcttaaat
    10441 acatttactg gtagtggtac agtttggcta gctccaactc aagtaatgta tgaaaaacta
    10501 agttttggag caatgcctac taatgataat atgaataata aagtttctaa gcaaaaatag
    10561 ttgttttata aatatttatt aaatcattat tgattttata aagtcactat aaattttatt
    10621 agattattat aaaatcagag tgtggaaata tttatgtgta ttctttaaat aaaaatttcc
    10681 acattctgta aagttattat acttgatata taaaattcaa tttaggaaca tagtctaaca
    10741 tttataaaca tattaaggta atgatttctc ttggtagtta ttctcatatc ttattctttg
    10801 attatattta tcttattcct agcccttatg tcatattata tttcttatct tattatttat
    10861 aattagcatt aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat
    10921 taatttcata aaacaatcaa ggtagtcaag agattcaact ttatttgatt ttaaaaattt
    10981 aagtgaattt gttcttatac acaccttttt aaaattatat ctgtagaaaa tatcatttag
    11041 agctataatt aaattaactt attaaatttt agaaaattta ttaaaaaata taagttaaat
    11101 taatattttt aaagattctt ttttgtaact actagaacac taatttagta gttattgtat
    11161 ctctaaagtt taagcatgga tagatataca tttgataatt tgcctataat ttacctacta
    11221 cttggattat tggagcaaat acaatatatt aataataagt tcctaaaacc aataaattaa
    11281 ctaatacaca taaagatatt gatgttgatt atacaactat aagaaacgga tttaacagaa
    11341 tttaatcaag ctaagaaatt atatatatcg tgatgttttt gaattagaag gtgattcaaa
    11401 attagtttat agtcttctgt gaatccaata aaaagtacag ggtacaagaa ttgttagtaa
    11461 aatttttaaa gattttatgg ataaatataa atagttttac ttattttcta aaactttaaa
    11521 ataccattaa ttaccttaaa tattttaata ggaggacata tgaatttaaa taaagttaaa
    11581 tttactgaag aacatgctat tgaagttact aattggaaat atgaaggtaa atattcaata
    11641 tacaatctac ctccatggga ggaaattaaa aagaagaatt tttcactagc aaaagaagat
    11701 aaaagaaaaa attttataag ttttataaat gacaataaag aattgatagg ttttataaac
    11761 ttattagatg aaggaagctc tgtattcttt ggtattggtt gaaaccgaat tattgtggaa
    11821 tgggaattgg taaagaaata attagcctag gattacaaga atgtagaaat aaatatagta
    11881 ctaaaccaat tgtattaaat gtaagaacct ggaacatgag agctgtaaaa tgttatgagt
    11941 ctcaaggatt taaaatagtt gagactaagg tacaaaaaac gcatttagga gatggagaat
    12001 tttttgttat gaggtatcaa tagtttataa caatagtcct attgttaata caacaattaa
    12061 aatgaaaaaa tacatatgtg taggtcatag caataagtgt ctttgaaata taaatttgag
    12121 agcttttgat aaaattcggt atacatatat attttatact acggatatat aaatctatct
    12181 acatacataa agagattgta agtaggtttg tgtatataaa aatagattta tatttcataa
    12241 atgtactttt tatttgcatt acctttattt tatctaagct ctcattttat tatctaatct
    12301 tcctttttac tggttttatt aagagagatg caattatcaa cacaacactt ccaagtatca
    12361 gccaatattt ctcatcaaaa ctaccttgac tcatagacat tataaaaaat tttccaacat
    12421 acttgccttt agtcatcata tttaaaatta aatatcctat agatatcata taacctatga
    12481 ttgtattatt aaataaattt gagaaaaaca ttcctaaact tcctataaac atagctgatg
    12541 cgaaacttcc aataatatac tcaactactg gaaaattaca tccaagatat attaaaacat
    12601 ttacaaaacc tatgattaat accactatac ctataattga catcagtatc ctaattaatt
    12661 gtacaataac ttgagataat gcttttgaag atactatctc atcaatttct tttgattgct
    12721 ctagcgaaaa tacaggtacc aacattacta tccctattat tgcaacataa ttttctaaac
    12781 atatagctgt tgaaattctg tctaaattag ctactccttg tattagagga atcatacata
    12841 gcaatataat tgaacatatt aaattagtta aagtgcttaa tttgaagttt tcttttatta
    12901 gaactaaaca gttttgaaaa ctttgcccaa acatcaagat tcccccttct tttttgctca
    12961 tataagaata tagctaacaa aacaagcacc aatgctccca aacttatcac aattctatta
    13021 atcataattt gattgaagtt ttgtataata atatccccat ttaatacact attatgtctt
    13081 atcattagcc ccattgttga ataatcacct ttgagtcctg tagaatttat actgtaaagc
    13141 caccaaatta attgcacaat aattgctact ggtgtatctg ttaatatagt aatactcatt
    13201 cctacagctg taactatcat aagagttggt agtatccacc ataatgtata ttttataaat
    13261 gctaagttat caatagatat acctttatct ttagcaaaga aaatcaaagg aagtaaatct
    13321 ttcataccta aaattaatac aggcaatatc atcataaaga caagtgcaaa atatcttgat
    13381 aaaactattt tgcatgaaga tacttgtttt gaataaataa tctctctcat tcttgaacgc
    13441 ttatctttaa gtgacataaa aactgccaca aatactggaa atatacttaa aactatacct
    13501 atataatcgc aaaaaattct tgcataccca ttactaactt tatctttttg tattgtatag
    13561 ttgtaattct tcatagcttc atcataggta actggaacta ctccaaatct ttgtaacttg
    13621 tcaaaagcat atgcagaccc tcctcctaaa atgtcgtcta ctctttgcat tatttcttca
    13681 aatctttcaa gagatacttt agaataatct atttttatac tttcatcagt tttccaaagt
    13741 gaatttattt cgctaatact tttaccagtt atttcttcta tatatttttc tatggtcttc
    13801 gttttatttt ggtctaattt tatatactta actacccctg ttggatacgt tgtatatgtg
    13861 tttttttgat attctctaat taggtttata gttgcatttg gaatgacttt ttctgttttc
    13921 atagattctt tttgaccata tggatattga ttgtgtacag agtaatattc ctcaatattc
    13981 tctacttttt gtggagcaac taatggattt ccatcattag gaatattttt acttacatca
    14041 tttgaaactg aacttccaag ttgagaaata tagaaaaaaa ctaaaactac aataaaaata
    14101 taatatatca tactctttaa tatattttta cattctttta taaatagatt catatattat
    14161 ctcctcccat tataagcata tagccatctt caatagttgg attacaattt actgctgtct
    14221 caaaaggttt atcatctgat ataatcctaa gtatagcctt tccaccatga gtcaacattc
    14281 ctataactat aaatctactt tttatatttt caatatcttt tttatctact tcaatactat
    14341 atactttccc ttcagctagt tttgctaatg cttctgttga gccattgtat atgacttttc
    14401 cactattcaa tatagctacg ttttcacatg tagcttctat atctccaaca atatgtgtag
    14461 ataaaataac gattctatca tttgcaattt cgcatagtaa atttctaaat cttatacgtt
    14521 cttctgggtc aagacctgct gtcggctcat ctactattaa aactctggga ttatgaatta
    14581 atgcttgtgc tatcccaagc cttctattca tacctccaga taaagatttt actttcacat
    14641 tttgataatt agtaaggttt actttgctta ataactttgc tataattttt cttctttttc
    14701 ttagtggtat atccgataaa actgctaaat aatccattgc tttatatact gtcatgttag
    14761 gatacattga aaagtcttgt ggcaaataac caaccatatt tctaatttcc ttagtattct
    14821 caataggaac tccacacata tgtattgaac cttctgtttt tgataataaa gtggctaaaa
    14881 ttctcataaa tgtggttttt cctgctccat ttcttcctaa aagaccaaac ataccactgt
    14941 cgatttcaag atttatatta tcaagagctt gttttttccc aaatctttta ctaagattgg
    15001 atactgctat tttcatttcc atatataaat acacccccag aaacaatttt atagaatttt
    15061 atttttataa atttttactg tattttcata ataaattcta aaacttaaga tattatttat
    15121 aaatttctaa a
//
LOCUS       DAMJWS010000095        14802 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMJWS010000095 DAMJWS010000000
VERSION     DAMJWS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.099"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            186..1589
                     /locus_tag="PC399_003140"
     CDS             186..1589
                     /locus_tag="PC399_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593982.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            1616..3160
                     /locus_tag="PC399_003141"
     CDS             1616..3160
                     /locus_tag="PC399_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2593983.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKSGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            3191..4582
                     /locus_tag="PC399_003142"
     CDS             3191..4582
                     /locus_tag="PC399_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF2593984.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            4775..6271
                     /gene="abfD"
                     /locus_tag="PC399_003143"
     CDS             4775..6271
                     /gene="abfD"
                     /locus_tag="PC399_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF2593985.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            6406..6681
                     /locus_tag="PC399_003144"
     CDS             6406..6681
                     /locus_tag="PC399_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2593986.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            6705..8012
                     /locus_tag="PC399_003145"
     CDS             6705..8012
                     /locus_tag="PC399_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2593987.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            8032..9150
                     /locus_tag="PC399_003146"
     CDS             8032..9150
                     /locus_tag="PC399_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2593988.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            9437..10357
                     /locus_tag="PC399_003147"
     CDS             9437..10357
                     /locus_tag="PC399_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF2593989.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            10472..11629
                     /locus_tag="PC399_003148"
     CDS             10472..11629
                     /locus_tag="PC399_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2593990.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(11710..12606)
                     /locus_tag="PC399_003149"
     CDS             complement(11710..12606)
                     /locus_tag="PC399_003149"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2593991.1"
                     /translation="MLKKVKYPIGQVKVSDKFKTIKLLSRKYSISSMCKLISVSRSGY
                     YKWLSYSKKSSDRGIKDRIIKDYIIEIHKKYRGTYGRKRICTYLNKILDTPINHKRVY
                     RLMKELGIKSIIRKKVYRRKFKSYEIYDNILNREFRANQPLEKICMDITYIPIGKKFL
                     YMNVAKDLFNGEIVAYEISTKMDTKLVNNTVNQLINMNLAKDCILHTDQGSQYTSRSY
                     SKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPKEMISLINDYIYF
                     YNNERIQLKNGMSPIEYRTHSE"
     gene            complement(12564..12881)
                     /locus_tag="PC399_003150"
     CDS             complement(12564..12881)
                     /locus_tag="PC399_003150"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2593992.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAFDDETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKLSTLLD
                     KSK"
     gene            complement(13018..14517)
                     /locus_tag="PC399_003151"
     CDS             complement(13018..14517)
                     /locus_tag="PC399_003151"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF2593993.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIEDEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         5605 a         1828 c         2678 g         4691 t
ORIGIN      
        1 aattatctat taaaaaataa aaattatttt tacaattaaa ttgttaatta aatcataaag
       61 aaaaaagtga tattaacaag ataaaatttt tttaatcaga aatgttaatt taataacaaa
      121 atagagtatt ggggagaaag gagaaatata ttttttaata atatttgtta atttaataac
      181 gaaaaatgaa agggagtgat ttagtgaagt ttgaattgat ggatttttta atgaatcctt
      241 ttgtccttat gtttgctgca gtaattacag gaattttatt tggaaaaatt aaatttggga
      301 agtttaattt tggggtatca ggagcattat ttacaggatt atttattgga tggcttgcat
      361 atagcttggg gaatctaata atagcaaaag gggaaacagc agcaggatat aaggcagcaa
      421 ctgtaatgat ggggaatgga attatttctt ctgacttctt tgatttcttt ttaataatat
      481 ttgttgcagc agttggtcta ttggcagcaa aagatatgaa agctgtatta aagaagtatg
      541 gagcaagatt tgtaatttta ggagttctta taacttttat aggtggctct atgacttatg
      601 caatgacttt actatcaagt gataaaggaa gtagtgctta tgaagtttct ggcgtttaca
      661 caggagcatt aacaagttcg cctggattag ctgctgcttt agaaacagct ggtaaacatg
      721 cagaagatgt atcaaaagaa tttgaaaaag cttcaataaa agataaaaaa gaaattttaa
      781 aagtagtcga cccagaaggc aaattggatg tgaatacaac tacatcactg actcaagagc
      841 aaatagataa gtatattgca tatgctgaag ctggtgttgg tataggtcat gcagtcgcat
      901 atccttttgg agttttaata gtaatcttag gtgtaaattt ccttccaaaa ttatttagga
      961 tggacctaaa agaagaaaga agaaaatatg agaaggaaat gaaggaagca agagatagtg
     1021 tttctggtaa aacgatacca gaagttccat ttaatataat aacatttttc ttgacatgtt
     1081 tagctggata cttagttgga ggaatacatg tatttatggg accactaggg tacttcacac
     1141 taggtgcaac aggaggttca ctaattgtat ctctagttct tggatatata ggtaagatag
     1201 gggtagtaaa tttcagaatg gaagaaaagg ttttaaatat tttaaaacaa atagggctag
     1261 tgtttttctt agcaatagtt ggattaagat atggtggaaa agtagttgat tcaattatga
     1321 catctggtat gcacctagcc ttagtagcaa tagcagtagg tgttacagct atgatgatag
     1381 gattcttagt tggcaaatac gtatttaaat taaattggat tttattatct ggagctgtct
     1441 gtggtggtat gacatcaaca ccaggtcttg gagctgctgt tgatgctctt gatagtgatg
     1501 acccagcagc aggttatggt gctacgtatc catttgcatt attaactaaa gttatattgg
     1561 ttatagtttt acacaaactt ccaatgtaaa taagatttta tatgggggga tatttatgaa
     1621 taatgaagga gcatttgaag aattaagtgg aagattaaga aataaagaat tgtgtgattt
     1681 aataatgaat gcagatgaag ctgctaaaat tatagaggat ggcatggtta ttggagttag
     1741 tggatttaca ccttcaggct atccaaaagc agtaccatta gcagtttcag aaagagctaa
     1801 aagtggagaa aagataaaat taaccgtata ttcaggagca tctttaggtc cagaagttga
     1861 tggagcatgg tcagaagcag gtataattga aagaagactt ccttatcaaa ctaattcaat
     1921 acttagaaat aatattaata aaggtgttgt tgattatata gatatgcacc taagtcattc
     1981 tactcaattt ttaaattatg gaactatacc taaagtagat gtagctatag tagaagcact
     2041 tgcaataact gaagaaggga atattatacc aactagtggg ataggtaatt ctccatcttt
     2101 tataaaaagt gcagataaag taatagtaga gataaatctt gcaaaaccaa tggaaatgga
     2161 aggtatggca gatatatata ttacggaaaa tccaccaaat agaaaaccaa tagaaataaa
     2221 tcatcctaag gatagaattg gaacaacata tataccttgt ggtttggata aaattgcagg
     2281 agtagtaatt accaatatgc aagataagac ccgtccattg ggggttgtag atgaagcttc
     2341 gaaaaaaata tcaaataata ttattgcttt tttaagagaa gaagtaaagt caggcagatt
     2401 atcaaagaat ttacttcctc ttcaatctgg tgtaggaagt gtagcaaatg cagtattata
     2461 tggattatgt gaatctgaat ttgaaaattt aacttgttat acagaggttg tacaggactc
     2521 tatgttggat ttaataagaa tgggtaaagt tacaatggca tctactacat ctgtaagtcc
     2581 atctccagaa ggattaataa aatttgaaaa agatattgat ttttttaagg ataagattat
     2641 attaagacct caagaaatta gtaacaatcc agaaatagcc agaagaattg gtgtaattgc
     2701 aatgaacact gcaattgaag ttgatattta tggaaatgta aattctactc acataatggg
     2761 gtcaaagatg atgaatggaa ttggtggttc tggtgatttt gctagaaatg gagcaataac
     2821 tatatttagt actgagtcaa ttgcaaaaaa tggtgatata tcatcaatag tacctatggt
     2881 atctcacgta gaccacactg aacatgatgt aatggtaatt gtaacagaac aaggatatgc
     2941 tgatttaaga ggtcttgcac ctagagaaag agctataaaa ataatagaaa attgtgccca
     3001 tccagattat aaagaacaac ttagggatta tttaaataga gcttgtaaaa gtggagcaaa
     3061 acaaacacct cacatattgg acgaagcttt atcatggcat agtaaattta tgagtacagg
     3121 tacaatgaaa aaggctgaaa cttttaaaag tgcattataa aatatttaat aaattggagg
     3181 tagattttaa atggaaaaag cagtggaaaa ctttgaagat ttaagtaagg aatacattaa
     3241 tggttatatt gagagagcaa gaaaagctca aagagagttt gaatgttata ctcaagaaca
     3301 agtagataaa atagtaaaaa tagttggaaa agtagtgtat tataatgctg aatatttagc
     3361 taaacttgct gtagaagaga ctggaatggg agtatatgaa gataaggtag ctaaaaataa
     3421 aagcaaagca aaagttatat ataacaattt aaaagataaa aaatcagttg gtataattga
     3481 tatagataga gaaactggaa taacaaaggt agctaaacca gttggagtag tagcggcaat
     3541 aactccttgt acaaatccaa tcgttacacc tatgagcaat gcaatgtttg cacttaaggg
     3601 aagaaatgca attattatca cacctcatca taaagcaatt ggatgtagta caaaaactgt
     3661 agagatgatt aatgaagaat tagaaaaaat aggagctcca gaaaatttaa tacaaatact
     3721 tgaccaacaa tcaagagaaa atacaagaaa tttaatttca tcagcagatg tagtaattgc
     3781 cactggagga atgggaatgg taaaagctgc gtactcaagt ggaaaaccag cacttggtgt
     3841 aggggcagga aatgttcaat gtatcataga tagagatgta gacattaaag aagcagtacc
     3901 taaaatcata gcaggaagaa tttttgataa tggtatcatt tgttcaggtg aacagtcagt
     3961 aatagtagca gaagaaatgt ttgataaaat aatggatgaa tttaaaaata ataaaggttt
     4021 tatagttaga gataaagtac aaaaagaagc tttcagaaat gctatgtttg taaataaatc
     4081 tatgaataaa gatgcagtag gtcaatcagt tcatacaatt gcaaaaattg caggtgttga
     4141 gataccagaa gatacaaaaa taatagtaat agaagctgat ggaccaggag aagaggatat
     4201 tattgcaaaa gaaaaaatgt gtccagttat atctgcatac aagtataaaa gttttgaaga
     4261 aggtgtagct attgcaaaag caaatttaaa tgtagaagga aaaggacata gtgtatctat
     4321 acattcaaat acagttaaaa atatagaata tgcaggagaa aatatagaag tatcaagatt
     4381 tgttattaat caatgttgtg ctactagtgc tggaggaagt ttctttaatg gtcttgcacc
     4441 tactaatact ctaggttgtg gttcttgggg aaataacagc atctctgaaa atcttgatta
     4501 taagcatcta attaatattt ctagaattgc ttattatatg ccagaaaatg aagtccctac
     4561 agatgaagaa ctttggggat aaaatttaga caaaaaatat tcattctaaa ttaaagtagg
     4621 aatatatttt aatttagaat gaatttaaga cataaattaa atttaaatta gtttattgga
     4681 ctaaatttaa aatattaaga gacatataga agaaggaagt attataattt aaaataaata
     4741 aaaaaatcta taaaagagaa ggagtggttt tattatggca ttaatgacag gagctcaata
     4801 tattgaaagt ttgagaaaat taaatactaa ggtttatatg tttggtgaag aggttaagaa
     4861 ttgggttgac catcctatga ttagaccatc tataaattgt gtagcagcaa cttatgactt
     4921 agctcatgac ccagaatatg cagatttaat gacagttact tcaaatatca caggtgaaaa
     4981 aataaataga tttggtcatc ttcatcaaag tgttgatgat ttgataaaaa aagtaaaaat
     5041 gcaaagacta tgtggtcaaa aaacagcttc ttgtttccaa agatgtgttg gtatggatgc
     5101 gtttaatgca gtgtattcaa caacatttga gtgtgataaa gcccatggaa ctaattatca
     5161 tgataatttt gtaaaatact taacttatat acaagaaaac gatttagtag ttgatggagc
     5221 tatgactgac cctaagggag atagaagtct atcacctagt gctcaacctg acccagatat
     5281 gttccttcat atagtagaaa gaagagaaga tgggataatt gtaagaggag caaaggcaca
     5341 tcaaactggt tcaataaatt cacatgagca ccttataatg cctactataa gtatgactga
     5401 agcagataaa gactacgctg tatcatttgc agttccttca gatgctgaag gagtgtttat
     5461 gatttatgga agacaatctt gtgacactag aaaattagaa gaaggtgcag atgttgattt
     5521 aggaaataag gagtttggag gtcaagaagc tttagtagta tttgataatg tatttgttcc
     5581 aaatgataga attttcttat gtggagaatg ggatttctct ggtatgttag tagagagatt
     5641 tgcaggatac catagacaaa gttatggtgg atgtaaagta ggagtaggtg atgtaataat
     5701 aggagcagca gctttagcag ctgattataa tggagcaaac aaagcttcac atataaaaga
     5761 taaactaata gaaatgactc accttaatga aagcttatat tgctgtggta tagcatgttc
     5821 atcagaaggt cacaaaacag aagctggaaa ctatcaaata gatttacttc ttgcaaatgt
     5881 gtgtaaacaa aatgtaacta gatttcctta tgaaatagta agacttgcag aagatatagc
     5941 tggagggctt atggttacta tgccttcaga aaaagacttt aaatcagacc ttaaagttgg
     6001 aacaagtgga atgactattg gcgaagtatg taacaagtat tttaaagctt cttcagtggc
     6061 ttcaacagaa gaaagaatga gaatattaag attcttagaa aatatatgcc ttggttcatc
     6121 tgctgttggt tatagaactg agtcaatgca tggggcaggt tcaccacaag ctcaaagaat
     6181 aatgatttca agacaaggaa acataaatca aaagaaagaa ttagctaaga aaattgcggg
     6241 aataaaaaaa gaggaagctt taaatttata aaaagttgat atataaaggg aaattatata
     6301 ttaatgtgta ttaattaatg aaaaaatatg tagtataaga gatagttata aaattatggt
     6361 ggtttttagc taagtaaaga aatttttaat aaggagaatt tacacatgac ctgtggagtt
     6421 agattttgtg ggggctgtaa ccctagatac gaaagaggga aggctcttac aataatcaaa
     6481 gagcacttca atggtagggt agattttagt atagcacaag agaacgtcga gtatgatttg
     6541 ctacttataa ttggaggatg cgcaactcta tgcgcatcct atcaacaata taaagtgaaa
     6601 aatgaatgtg ttaagatgtg ggattttagc catatagaaa gagttataaa tgtgattgaa
     6661 tcagcaattt gtgaaactta aaaaagctaa atggggggat ttcattgagt tggcaagaat
     6721 tgtatcaaag taaattatgt agtgctacag aagctgtaaa acagattaaa aacggtgata
     6781 ctgtagtatt tgcacattgt gtaggtgaac ctcctgcact agtagaggct atgattgaaa
     6841 atgctgaaca atataaagat gttgagatta aacatatggt aagcttgggt agtggtggat
     6901 atactgctaa gggaatggaa gctcatttta gagtaaatcc aatgtttgta agtggaaatg
     6961 taagaaaggc aattgaaaat ggagatggtg attttacacc tgcattcttc catgaagtac
     7021 caaagttgct tagagaaaaa agattaaaat gtgatgttgt tttagcacag gtaactccac
     7081 cagatgaaca tgggtattgt agcttaggaa catcagttga ttatacttat gaagccatta
     7141 aaactgctag aacagtaatt gttcaagtga atgaccagtt tcctagaacc tatggtgagg
     7201 tagtccatgt atcagagttt gactatatag tggaaaaatc acaaccttta tttgaactac
     7261 aacctgcaaa gattggagaa gtagaagaag caattggtaa aaattgtgct tcattaattg
     7321 aagatggttc tactttacag ctagggattg gtgggatacc agatgcagtt atgttatttt
     7381 taactgataa aaaggattta gggattcatt cagaaatgat ttcagatggg actttggccc
     7441 tttatgaaaa aggtgttata aatggtaaat ataaaaattt tgataaagaa aaaatgacag
     7501 ttaccttctt aatgggtact aaaaaattat atgactttgc aaataataac cctgcagtag
     7561 aggtaaaacc agtagactat gtgaatcatc cagcaattat aatgaaacaa cataagatgg
     7621 tttctataaa ttctgctata caagttgatt taatgggaca ggtagttgca gaggctatgg
     7681 gacttagaca atttagtggt gttggaggtc aagttgactt tatacgtgga gtgtctatgg
     7741 gtgaagatgg aaaggcaatt atagctatgc cttcaataac tacgaaaaaa gatggtacag
     7801 taatttcaaa aatcgtgtct attgtggatg aaggtgcacc tataacgaca tcaagaaatg
     7861 atgttgacta tattgtaaca gaatacggta tagcagaatt aaaaggaaaa tctcttagag
     7921 aaagagcgag aaatttaata aatatagctc atccaagtgt aagagaaagt ctagcagtag
     7981 aatttgaaaa gagatttaag gagaaatatt aataatagag gggtgttaat aatggaagca
     8041 cttagagtag ttccaaagat attttacttt gatacattta aagaatttaa tgaagaattt
     8101 aaaataggaa aaaatgattt agtaattact aatgagttta tatatgagcc atatatgaaa
     8161 cctcttggaa tagatacaaa tttaattttt caagaaaagt ttggaacagg tgaaccatct
     8221 gatgaaatga tagactccat gacaaaggaa atgaagaaat acaattttga tagaattatt
     8281 gcatttggag gaggaacaat agttgatata tgtaagatat tagcacttga tgttccagaa
     8341 aagtctattg atttatttga aggagaagta tcacctaaaa aagtaaaaga gttagtagta
     8401 gttcctacaa catgtggtac aggtagtgaa gtaacaaatg tagctattgc agagttaaaa
     8461 tctaaacata ctaaaaaggg gcttgcagta gaagaaacat atgcagacta tgcagtgtta
     8521 attccagaga cagtaaaagg gttaccttac aaattctttg ttacaagttc ggtagatgct
     8581 cttatacatg ctattgagtc ttatctttct ccaaaagcaa gtccatttac agagatgtat
     8641 tctctacaag caataaaaat gattatggat gggtataaga agatagttga taaaggagaa
     8701 gaagagagat ttaatcatct tagagacttt gtacttgcat caaattatgc aggaattgct
     8761 tttggaaatg ctggttgtgc agcagttcat gcactatctt attcaatagg tggagctttc
     8821 catgttgcac atggtgaagc aaattatcag ttctttactg aagtatttaa gatgtattct
     8881 agaaaaaaac ctaatggaaa aattataaaa tgtacaaaaa ttttagcaga tgcccttgaa
     8941 tgtgacccta actgtgatgt ttatggagaa ttagaaaagt ttttaaataa attaatagct
     9001 aaaaaagctt tgagagaata tggaatggtg gaaagtcaga tagatgaatt tacagatagt
     9061 acaatagcaa atcaacaacg actacttgct aataattatg tagagcttag tagagaggaa
     9121 ataagagaga tatttgcaaa tctttattaa tacaattttt tatttatatg tatagtcaac
     9181 ataaacaatg ctaattatat ttatgtacaa ataaaaataa gggaacttaa attttaatta
     9241 aagttaagtt ctcttatttt tatatggagt taaatataag tatattttta atttaaaata
     9301 tgcttatatt taagtctatt actttatttg aaacaatttg tagaatttca aattccttga
     9361 cagtaataag taagtaattg tataatcaaa ctgcaatata ttaataatat tagtgaaaat
     9421 gaaaggatta taaaatatgt ataaatcaga aaatttttca aaaaaatctg tattaataaa
     9481 ttcattttta agtaaatttt taaaaataat tggatggggt ttggtaagct tatttacatt
     9541 atcagcctta gtactttcaa ctatttccat tataattgga tgggctgtaa tacttcctat
     9601 tcaagtatgt gcagtatttt tacttatagg aggagtttgt atttttaaag gtaagagatt
     9661 aggagaccaa atttctcgtt ataaaaaata ttgtacaatt ataaacaata gaaatattat
     9721 tccagtagaa cttattgcag aaaacacatc taaatcatta aattttataa taaaagatgt
     9781 acaaaagatg atagacaaaa actactttat aaatacttat attgataaaa gaaataatca
     9841 gattgtgtta acaaatgaag attttgttcc accaagatat gaagatgtag catatgaagt
     9901 caaagaagaa gaaaagactg atgaatccaa tgaagttgat acaattataa ataaaggaat
     9961 gaattatcta acacaaataa aagaagctaa taaaaatatt aaatcagaat caatgggcga
    10021 taaaattgta caagtagaag atgttacaag taaaatattt gatgttgtaa aacatgaccc
    10081 atcaaaactt actcaaatac aaaaatttat ggattattat cttccaacca ctttaaaact
    10141 attaaattca tatcatacat tggaagaaca aggaattgat agagaaaata taacaacaac
    10201 tatggaaagt attgaaaata caatgaatac gattgtagta gcttttgaaa atcaattgga
    10261 ctatctgttt gaagatgaag ccattgatat ttctacagat attactgttt tagagaatat
    10321 gttagtccaa gaaggattaa cgagtggtaa attttaactt caatagaaaa aatattctgt
    10381 tatttttaaa caattaatta gtcataaagt ataaaatgtg tattatataa gttaatcagt
    10441 actttatgaa ggaagaggag agaaagatac aatgaataat ttagatgata taccagttat
    10501 gccaactttg acacttgacc catttggaga agaatcaaac actaatgata tagataattc
    10561 tgacttatta atgaaaaagg atgaaaaaga cccagaagaa gaaaaactgt ctgaatctga
    10621 acgcaagatg gtaaaggaat ttgcggaaaa aatagatatt acaaatacaa atatgatttt
    10681 gcaatatgga gctggtgctc aaaaaaaggt agcaagtttt tctgaaacag ctttaaagag
    10741 tgtaaagaca agagatttag gagaagttgg agatatgctt acaaatctag ttactgattt
    10801 aaaagcattt tcagcagatg aaaaagaaca aagtggtttt ttaggaattt ttaaaaaggc
    10861 aaataataaa atttcaaatt taaaaactaa gtatgatagt gcagaggtaa atgtggataa
    10921 agtaagcaaa gagcttcaaa agcatcaggt taaaatattg aaagatatag caatgcttga
    10981 taagatgtat gaattgaact tagcttactc aaaagagctt actatgtata ttatagcagg
    11041 aaaacaaaaa ttaaaggata tgaaagaata tgagatgcca aagttgagag aaaaagctcg
    11101 tttatctggt tcaacagaag atgctcaatc tgtaaatgat atggtttcac tttgtgacag
    11161 atttgaaaag aagattcatg acttagaatt aactagaatg gtatcgcttc aaatggctcc
    11221 tcagataaga ttggttcaaa atagtaataa tctgatggca gaaaagattc aatcaactat
    11281 agtaaatacc attccattat ggaaaaatca aatagtgctt gctcttggaa tttctcactt
    11341 aaatcaagct atgaaagcac agcgtgaagt tagtaatatg acaaatgagc ttttattaaa
    11401 gaatgcagaa actttgaaga tgggaacaat agaaacagca aaagaatcag aaagaggaat
    11461 tgtagatata gacacaatta agaaaactaa tcaatctcta atatctacta ttgatgaggt
    11521 agttaagata caacatgatg gtagaataaa gagacaagaa gcagaagttg aattatcaaa
    11581 aatagaaaat gaacttaaaa gcaagcttct tgaatttaca gttaaataaa aattatacta
    11641 agataaaaag agactgtctc aaaattggat atgtccctgt caagtagaca gacttaaaaa
    11701 aggctatctt tactcggaat gagttcgata ttcaatcgga ctcattccgt tttttaattg
    11761 tattctttca ttattataaa aataaatata atcatttatt agactaatca tttcctttgg
    11821 atctgtagta tcaattaaat atattaattc agatttaaaa tgactgaaaa aactttctat
    11881 aggagcatta tcccagcagt tgccccttcg ggacattgat tgtataatac cattatcctt
    11941 aagcctttta gagtaagatc tacttgtata ttgtgaacct tgatctgtat gtaaaataca
    12001 atctttagct aaattcatat ttattagttg atttactgta ttattaacta atttagtatc
    12061 catttttgta cttatctcat atgccactat ttcaccatta aataaatctt tggctacatt
    12121 catatataag aattttttac cgataggtat gtatgttata tccatacaaa ttttttctaa
    12181 tggttgatta gctctaaatt cacgatttaa tatattatca tatatctcat atgatttaaa
    12241 cttacgtcta tatactttct ttctaataat agatttaata cctagttctt tcatcaacct
    12301 atatactctc ttatgattaa taggggtatc aagtatttta tttaaataag tacaaattct
    12361 ttttctacca taagttcctc tatatttttt atgtatttca attatataat ccttaataat
    12421 tctatctttg atacccctat cggaagattt tttagaataa cttagccatt tatagtaacc
    12481 gcttcttgac acacttatca acttacacat actagaaata ctatacttcc tagacagaag
    12541 tttaattgtt ttaaatttat cgcttacttt gacttgtcca atagggtact taactttttt
    12601 aagaattcat tctccatgcg taagtacttc aattcttctt caacagattt aaattttcta
    12661 ttatcatttt ctagactaat atttttaaat ctgcccctag tttcatcatc aaatgcagtt
    12721 ttgcctttat ttttataatc ttttacccag ttttgaactt gtgttttgct tcttatattc
    12781 agcatctcag caattttgta tgatgagtat ccttctttta tatataagtt tacagctttt
    12841 agcttaagtt ctttgctata ttttttgtgt tttttagaca tagaaaaacc cccttagagt
    12901 atacgctttt tagacctatt ataatagcct tttatttaaa tgtctactat aaggggatta
    12961 tactaaaata gagataagtc tctttttcat tgtttaaatg attaaagtat atttatatta
    13021 taaatttcta ttatctttta acataacatc atgagctcca ccttcaacta tactagtaga
    13081 agatacaagt gtaagtttag catttttttg aagttcagga atgctaagtg ctccacagtt
    13141 acacatagta gaacgaactt tacttaaaga aagagttaca ttgtctttca aagaaccagc
    13201 ataaggaaca taagagtcaa caccttcttc aaaagaaagt ttcttatctc caccaaggtc
    13261 atatctttgc cagtttctag cacgagcaga accttctccc caatactctt tcatataact
    13321 accatttata ttaactttgt tagttggact ctcatcaaaa cgagagaagt atctaccaag
    13381 cattataaaa tctgcaccca tagctaaagc taaagttata tgatggtcat aaacgatacc
    13441 accatcagaa caaattggaa tataaatacc tgttttttca aagtattcgt ccctagcttg
    13501 agcaacttca attatagaag tagcttgtcc acgtcctata cctttttgtt cacgagttat
    13561 acatatagaa ccaccaccaa taccaacttt aacgaagtca gcaccagctt cagctaggta
    13621 aaggaatccc tcacggtcaa ctacatttcc agctcctacc ttaactgtat caccatattt
    13681 ttcacgaatg aaatcaagtg ttattttttg ccactcagaa aaaccttctg aagaatctat
    13741 acataaaaca tcagcaccag cttcaactaa agcaggaaca cgttcagcaa agtctctggt
    13801 attaatacca gctccaacta catatctctt agaagaatct aaaagttcaa gtggattttc
    13861 tttatgagaa gagtaatctt tacggaatac catatacata agattaccat tgtcatcaag
    13921 tacaggaaga gaattaagtt tgtggtccca tataatgttg tttgcttcct ttaaagtaac
    13981 atctttgtta gcataaacta tagatgacat aggagtcata aattcactaa ctttagtatc
    14041 taaatccata cgacttatac gataatcacg acttgcaaca atacctaata atttaccagt
    14101 tgaagttcca tcttcagtaa cagctactgt agaatgtcca gttttttctt ttaagtcaag
    14161 tatgtctttt aatgtgttat caggtttaat atttgaatca cttacaacaa aaccagcttt
    14221 gtgggattta acacgtgcta ccatagcagc ttcatcttca atagtttgag aaccgtatat
    14281 aaatgaaata ccaccttctt tagcaagtgc aatagccatt ttgtcatcag aaacagattg
    14341 cataatagca gaagtcaaag gtatattcat ataaatatct gcttcctcgc cctttttaaa
    14401 tttagtaaca ggtgttttta aacttacatt agcaggtata caatcagtag aagagaaacc
    14461 tggtacaaga aggtattcac tgaaagtatg tgatggtgta tcaaaataaa acgccatttt
    14521 ttaatcactc cttatatttt tatacttttt ataattgata gtattaatta taacttattc
    14581 tttaatttta ttaaagtatt ttgtcaaata ttgagatttt tgaaaaattt aaactatttt
    14641 aattgaattt gtttaatata tattgcctta tagggtaaag ttgtatatta aataatgttt
    14701 agtgtttaat agtagctaat ttgaaattaa tctttagtta ttcatataat tacatttgta
    14761 tagtttatat tgataatatt gctagagagt gcataaacct ca
//
LOCUS       DAMJWS010000096        14317 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMJWS010000096 DAMJWS010000000
VERSION     DAMJWS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.100"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            182..403
                     /locus_tag="PC399_003152"
     CDS             182..403
                     /locus_tag="PC399_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2593994.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            403..666
                     /locus_tag="PC399_003153"
     CDS             403..666
                     /locus_tag="PC399_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2593995.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            728..1300
                     /locus_tag="PC399_003154"
     CDS             728..1300
                     /locus_tag="PC399_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2593996.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(2054..2404)
                     /locus_tag="PC399_003155"
                     /pseudo
     CDS             complement(2054..2404)
                     /locus_tag="PC399_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2721..3254
                     /locus_tag="PC399_003156"
     CDS             2721..3254
                     /locus_tag="PC399_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2593997.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPDFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGINRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            3480..3659
                     /locus_tag="PC399_003157"
     CDS             3480..3659
                     /locus_tag="PC399_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2593998.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNNYNIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4267..4884)
                     /locus_tag="PC399_003158"
     CDS             complement(4267..4884)
                     /locus_tag="PC399_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF2593999.1"
                     /translation="MDEKILELLQEMQGSISGIEGTISDMQSNINGIEGTISDMQSNI
                     NGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISSMDGTINS
                     MQDSITDMQVEIKEVNSKLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINR
                     NTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            5350..5763
                     /locus_tag="PC399_003159"
     CDS             5350..5763
                     /locus_tag="PC399_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2594000.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            5836..6372
                     /locus_tag="PC399_003160"
     CDS             5836..6372
                     /locus_tag="PC399_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2594001.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNGVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            6532..7194
                     /locus_tag="PC399_003161"
                     /pseudo
     CDS             6532..7194
                     /locus_tag="PC399_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            7740..7859
                     /locus_tag="PC399_003162"
                     /pseudo
     CDS             7740..7859
                     /locus_tag="PC399_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7986..8888
                     /locus_tag="PC399_003163"
     CDS             7986..8888
                     /locus_tag="PC399_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2594002.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            9500..10513
                     /locus_tag="PC399_003164"
     CDS             9500..10513
                     /locus_tag="PC399_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2594003.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPSDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            10869..11438
                     /locus_tag="PC399_003165"
     CDS             10869..11438
                     /locus_tag="PC399_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2594004.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYFNRYN"
     gene            complement(11774..13111)
                     /locus_tag="PC399_003166"
     CDS             complement(11774..13111)
                     /locus_tag="PC399_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2594005.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYTLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMIFYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(13466..14176)
                     /locus_tag="PC399_003167"
     CDS             complement(13466..14176)
                     /locus_tag="PC399_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2594006.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT         5207 a         1637 c         2360 g         5113 t
ORIGIN      
        1 atagatattt atgaaagatt aattagaaaa attttagagg taaaataaga aaaaaagata
       61 cttatttaat catttaatct tattaaagac ctattaaaac taccagttat aatatagaac
      121 cagtatacaa tttttaaaaa taatgctatt ttaaaattct aaaaattaag gaggataatt
      181 aatgggatat ttaaaagagg ataaacaaga ctattgttgt gaatttgcta gaccatacat
      241 caatcgtcaa atttatgatg acacatacaa tccaaaaaca gcattaaaaa gaggtacttt
      301 atttcctgaa ttagatttaa tagagtcaaa taactataat gattggctat atgcaaatcc
      361 taaaaataga gttagagcta aaaaaggggg taacatgtac taatgtcatt aaatagaaga
      421 gaacttttag agagaatttc agaatatcaa tttgcctgta tagaattaaa tttatattta
      481 gataacaatc caagggataa gaaagctctt gattcataca acagatactg cgataaattt
      541 acacaagctg tgtgtgatta tgaatctaaa tatggagcat taactaattt tggatatgaa
      601 tcaagtgaat atccttggtc atggatatct gaaccttggc catgggacaa atcattttat
      661 aaataaaatt attaaacttt aaaattagat ttattattaa atatatatta aggaggagta
      721 agataatatg tggatttatc aaaaaacact ggaacatcca gttaacataa gacaagcaga
      781 ccctagaatg gcaaaatata tcatgactca gttgggagga cctaatggtg agttggcagc
      841 tgcaactaga tatcttcaac aaagatatac tatgccaact ggaaaatctc gtgcactttt
      901 aactgatata ggtacagagg aaatggctca tgttgagata atttcttcag tgttatatca
      961 attaataggc aattgtactc cagaagagct taaggctgct ggacttggta gtaattatgc
     1021 taattttgga catggtcttc agccagtaga ttctaatgga gtaaacttta ctacaagtta
     1081 tattaatgtc tttggcgatt cggtaactga tttacatgag gatatggctg ctgaacaaaa
     1141 agcattggct acgtactatc aattaataaa tttaactgat gaccctgatt tgaaagatat
     1201 attgagattt ttgggtgaga gggaagtagt tcactatcaa agatttggtg aagcattaat
     1261 ggatgtttat gagtttacag agtgcaagca tcagttttaa tgaagaattg aaagtgaatg
     1321 gagaataagc atcattttta atgaagaatt aaaagtgaat ggagagtaag catcattttt
     1381 aatgaagaat taaaagtgaa tggagagtaa gcataagttc taatgaagaa ttaaaagtga
     1441 aaagagaata agcataagtt ctaatgaagt attaaaaatg aaaagagaat aagcatcatt
     1501 tttaatgaag tatcaaaaat aaaaagagtg taaatattag ctctgataaa ctatcaaaat
     1561 aagaggtaaa agttttagtt gatttctttt atctcttatt ttttattgat taaacagatt
     1621 actttgctat ttaaatatat tattttagca aaaaaagtcc aatcaaaagt tcaaagtatt
     1681 accatagttc aaagcattgc cgttgttagt aagtttttta caatattttt tatttactct
     1741 ttggataatc aaaagaaata aaaaatcata tctctttttt tatatttctg tacacttttg
     1801 gagaatatcc cgttttttct ttaaaacatt tactaaaata gattatttga tgaaaaacaa
     1861 cttttatagt aatgtcactt attagctcat ttgtgccttt tagaaattga gttgctttta
     1921 ataaacaaaa ctcagtaaaa tatttataag gtatagtatt taaacttagt tcaaatcatc
     1981 tggagcactc tgatttattg atattaccac tgcttgatag tactccaaaa ttaagttttc
     2041 acaatagtac ccttcaatat aatgtaaaac aatagtattt ttcaatataa cgtaaaattt
     2101 tttgcatatg gatattaatt atatttttgt gttttattgg tggcaaaaca atatttttta
     2161 tcataataag ccaaaggaaa gaaacagaaa ctaatgcttc atacatatac agttcatttt
     2221 tacttttttc taaccttatt aatcgttgta gaatagaaaa cactttattg cgtcaattat
     2281 atttatgtgt aaaatgataa atagcaaatt gttcgttttc tgttattcta tctactaacg
     2341 attttactgg atttctaaca taaaaatact tttctttttt acagcctatt cttacttttc
     2401 ccatttgaaa gtattatgat attacttgat gctatatcga tatttttttg gtctatttag
     2461 tgataaaatt ataagataca cttctataaa agttaataca gtaggaagct taaatgtatg
     2521 aattttatat ccatcaaagg gaaagggaaa aaaggatgaa cagatgcagg taaatataaa
     2581 actaatatga tatgaggata gatattataa cgaaagtgta gttagaagga aattatatta
     2641 gtatgttttt acataatggt agatagaaaa gaagtgtcta atgatttgag tgaagttttc
     2701 agaatataag gaggaattgt atgcaatata ttagctatta ccatagtcca attggaaata
     2761 ttcttttagc atcagataat attgggttaa ctggtctttg gtttgagaat caaaagtatt
     2821 atgcttataa tcttgaccaa gaacatcaag aaaaagaatt gccaattttc gagcagacaa
     2881 agaaatggtt agatatttat ttttcaggaa aagaacccga ttttacacca tcactccata
     2941 tggtaggtac tccttttcag actacagtgt ggaaaatttt acaacaaata ccatatggta
     3001 aaacaatcac ttatggagaa attgcatata aagttgctca acaaaaagga atatctaaaa
     3061 tgtcagcaca agcaattggt ggtgcagtag ggcataatgg aatctctatt attattccat
     3121 gtcatcgtgt tgtaggaaca aatgggagtt tgactgggta tgctggtgga attaatagaa
     3181 aaataaaatt gttatcttta gaaaaagtat ctgtgcaaca ttattttatc ccaaagaaag
     3241 ggagtgcatt ataaagccat taaatttaga aattcttaat catttatttg atatacaaac
     3301 atctaatttt gaaagcaata acttgcaatt ttaaaaaatg attatttaaa ctatatgtta
     3361 tacaacctta ttcctagtaa acattataca actttagagg tagtttttag agcttgtatt
     3421 ttggaagttt attttctttt ttcatgtgag tagttatatg tttagaggaa gtaatttata
     3481 tgctacaaac aggaaaacaa ggagcagaca aaaaatactt agataatatg ttttttgtga
     3541 aaggttatgt tgaagaactt ctatttttag acaacaatta taatattgta ttttcatgta
     3601 ttgtatttca tcattgtaca aatgttcaaa atatattttg tatattttgg aaatggtaag
     3661 agtgttaaat gtaagggaaa aatttataat gattgatact taagctactc attaagtatt
     3721 aagaaccata caagataagg ttgaaattta tatgtttcgt tttatgtaaa aaatgtaagt
     3781 gagttagaaa tgaaagaatt atttataaaa aatagattaa gtattggaaa atatgaaaaa
     3841 tagatgttga acaaaactta gtcgattagt taaatttaac caaacactta aagtatcaaa
     3901 agaaaaaatt attactctta agcaagaaga tataaatgga aaaatgaaaa caagatttta
     3961 tccttttata agtgataagg ggatgtgttt taatcaaaga tggactttta ttttaggctg
     4021 aaaaattata taactatatt ataggatata agggagatac acaacttttt agtgtccaat
     4081 ctcccttatt ttttattatt atatcaagac tcatttagtc gtgttaattt taggataaaa
     4141 tgagtatttt tttaataaat tatttataag taaagtgtgt tgtcggataa tcagtatttc
     4201 tgtataaaat ttggtataac tttaaagaag tatgtgaaat tttcaggtat tttttactat
     4261 ggtatattat ttaactgatt ttaatttagc aatatcattc gaattcttag atgtgactat
     4321 ctctatatta tttaaatcat ttttaatatc tgatgtatct tttactaatg aagaggtatg
     4381 tttaatcact gtatctaatg tatttctgtt aatttttaca tctgacttaa tttctgtaac
     4441 atcactctga atagtatcaa gtctactact agtttcaact tgtccagttt ttaagtcttt
     4501 aatatcaggt tccatattgt ctaacttact attgacttct ttaatttcaa cttgcatatc
     4561 agtaatactg tcttgcatac tattaatagt gccatccata ctactgatac tgccttgcat
     4621 atcgctaata gtgccattca taccactgat actaccttgc atatcgccaa tagtgccttg
     4681 cataccacta atagtgtttt cgataccatt gatattgcct tgcatactac taatagtgcc
     4741 ttcgatacca ttgatattgc tttgcatatc gctaatagtg ccttcgatac cattgatatt
     4801 gctttgcata tcgctaatag tgccttcgat accactgata ctaccttgca tttcctgtaa
     4861 taactctaat attttttcat ccatcactat cacactcctg ttttattatt ataacataat
     4921 ttaggtgtta tattccacaa acgctatgtc atatttaatt tttaatcata tgattatctg
     4981 ctattcaaaa catggttaaa tatatctata ttgagaactt ttttattaat gaatcttatg
     5041 tattgtctaa gtttctaaga tactgtaata agtgtttttg aggcatgtgt ggttatattt
     5101 gtatttaaaa ctgctggtaa tatcatatac ttttaaatat accttaagtt atttatattg
     5161 attacttgta tttgaataat ttgattagta gcttcctttt tatctctttt tattcttagt
     5221 tttaaacttt catatttcct tgaaaattta ttggcaatga aagtgcatga attttttagc
     5281 tattttttat gttacaatat gaatatataa caattcgaca aattggattt ttgaaaggag
     5341 taaaatgtta tgaaaatgcc aaaagagagt attgatacga ttatgtttgc tccttgtgga
     5401 atgaattgta tggtttgcta taagcattgt tatcacaaaa agccatgtgc tggttgtttg
     5461 aatggcgata caggaaagcc agaacactgc cgtaagtgta aaataaagga ttgtgtctgt
     5521 caaaaagggt tgttttattg ttttgaatgt ttcaattttc cttgtaaact cataaaaaat
     5581 cttgaaaaaa gttataacaa gagatatcaa gcaagcctta tggaaaatag taaatttgtt
     5641 cagcagtacg gtttagagaa gtttatgcaa aagcagaagg agagatatac ttgttctaaa
     5701 tgtggaggaa ttatatctat tcatgatagg gaatgtagcg agtgtcaaga aaaagtgaaa
     5761 tagctaaatc actgtttatc aagagagaaa aaattgtaat atatataaag gtggattata
     5821 ttgaggtggt atatagtgat aggtttaaaa tgtggtatgg ttaaattatt ggaacatcag
     5881 atgatatggg ataaaagtgc aaaagatgtc attatattat tgaaaagtat atgggataag
     5941 actgccattg atattcaaca cattggaagc acttctattc ccagtattag cgcaaagcct
     6001 attattgata ttgttgtagg tgttgcttct ttagaggaag ctaaattata cctcgaaagg
     6061 ttagagcaat gtggaatcgt ttttcgaggt caggatgtac caaaacaatt actttttgct
     6121 atgggagatt ttgagaaaaa tactcgtact caccatattc atgttgttga atggaatggt
     6181 gttgcatgga ataattatat caattttcga gattatctaa atacttttac agacaaagca
     6241 aaactctatg atagtcataa acaaaaattg gcattacagt ttgaggacaa tcgtaaaatc
     6301 tatacacaga gaaagcaaga actaataaat attttgctgg aagaagcacg actgtggaga
     6361 ataaatacct aaaagaataa atttacattt attgagaaat tgagcaatta ttttcttata
     6421 tttagtaaat aaaaatctat ttttgttgat taaaaaatat ctgtacacac tcatgacttt
     6481 agtcattgga gattcagatt gtgtagaatt attggatgaa ggaggaacag tatgaaaaag
     6541 gatacatctc atattatcaa acgggcagag cactttcagg taattttgat tggtgaagga
     6601 cttatggttg gggctgtggc tggtttgatt gtgcttttat acaggatact tttagggcag
     6661 gctggtactt ggcttagcca aattttaaaa tttgtagaag gttctacaat aaaaataata
     6721 gcttggtttg cagtacttgc agtacttgca tggattgtat caaagttggt ggactgggag
     6781 ccaatgattt ctggaagtgg tatccctcag cttgaggggg aaatgacagg gaaaataaaa
     6841 caagtatggt ggcgagtgtt gccaacaaaa tttattggag gattcttatc actcatgggt
     6901 ggtcttgctt tgggaaggga aggtccttcc attcagcttg gtgctatggc aggtaaaggt
     6961 atttctagag ctcttgaccg agggaagaca gaggaaaaat tcctgttaac ctgtggggca
     7021 agtgctggat tatcagcagc ctttcatgca ccactggcgg gtgttatgtt ttcacttgaa
     7081 gaagtccata aaaacttttc agtatctgtt ttaatttctg tgatgacagc atctttgaca
     7141 gcagactata tttcagccaa tttcctagga atggagtctg tattccagtt tgattaaaaa
     7201 atagctacgc acacttgtga ctttagtcat gagttagtag ctaaaatagt cagcatatag
     7261 ggaaacctat atgtagagat aggatagaac ctatccaata ctacttgaat tgctggaaac
     7321 ccctaaagct aaccaaacta aaacataagg atgaaataaa cctaagtgtg aaagttacga
     7381 aagtagaaaa aattggttag atggtataag gttaaatcct aagtacttta aaatgggcaa
     7441 tcagcaggta aactccgaat aggagaaact tcaacgacta ttcctcttga gggaagtaca
     7501 ctacaagcta ttggtagtgg aagtgggtag accttaacgg ataatgccga aggataagat
     7561 atagtctgtg cttgcatgaa agtgtaagaa gttcataaga gaactgcata ggtagtagcg
     7621 aacttatgtg aacgacaacc tcaaaaatga taaaagttaa gacttacggt tcttaaatat
     7681 gttatttaaa attaaatata aaaccttcta aataattgtt ttaaataatt acatttgtta
     7741 acattattat aagagaatgg atttgtccta gttgtaatga aactcacgat agagatataa
     7801 atgcaagtat aaatatatta aaagaaggat taagactaat aacaattcaa aataaataac
     7861 ttatgtaaga accgtaggaa ctacggggat agcctgtgga gaattagtaa gacatatgtt
     7921 agtatgcaaa attctatgaa gcaggaaccc tgtgacttta gtcatgggag gttcagattg
     7981 gaaatgtgtt gccacaagat tattatggat taatagtgat tttaggtgtt attcttggag
     8041 ttatgggggc attttacaac tggtttactc tatatgtgca gtctttatac aagaagattc
     8101 ctaaaattgg tacgtttgga aaactgttga ttccatttat aatggctggt attcttgggc
     8161 ttgttatgcc tgatgttctg gggagtggac acaatttagt tgaagaactt acaagtcatg
     8221 aatatatttt tggaatggtt gttttgattt ttgttatacg ttttatattt tcggctgtta
     8281 gttttgggtc aggtgcacca gggggaatat tttttccatt gcttgtttta ggagcattta
     8341 taggtggaat atttggtatg gtaggtgtta aagtttttgg aatgaatccc gattatgtaa
     8401 ataactttgt gcttttggct atggcaggat attttacagc tattgtgcgt gcccctttga
     8461 ctggtatcat attaattttt gaaatgacag gttctgttag tcaaatgctt tcactatcag
     8521 taatttcaat tgtggcatat attgtggcta cacttatgaa gtctaaacct atttatgaaa
     8581 gtcttcttga gaggttactt gaaaaaaatg gagaacctgt acataaagag aggggcgaaa
     8641 agatattaga ccagttctct gttatgtttg gctctcctgt tgcgaacaag gcaattcagg
     8701 atgtacagtg gcctgaaaat tgtcttttgg tagcaattca aagaggtgga gaagaaatta
     8761 ttcctagagg taagacaatt cttagaccaa gtgatgtaat tgtgactatg acagatgaga
     8821 gagatagtgg tgctgtgtat gataagatgg aaaatttgtg taaagaacat acgtcacata
     8881 ttttttaggg ataatgatga atgtttaata atgatagaat atttaatgaa gatttgatta
     8941 aaaaattggt aatttgaaag agagttaagg aattaatata agaaaatgaa aatgtgatat
     9001 cttagatgta aaagagtttg aagaaaataa ttgtatatat ttgttaatga gattatatgg
     9061 actaatggta tatttatact gtgctaaaag ttgcactatt gtatgaggaa tgggaataaa
     9121 gggtttgaaa aaataggtag aagatttttc agatattctt actgggatat tttaattaat
     9181 atattttaat tgcgatattt tgggaagaat agaatttatt tataaatgaa aataattaaa
     9241 aggggttaag gttgtattag ccccttttgc ttatagagtt tggaaatggt atcttgatag
     9301 cagttgtttc atgcttatta tattttatta catattttaa aattgacctt attactccta
     9361 aatcattgtt atatcaactt ttaaaacact ttcaagacaa tcgcctattg aaaacaaagt
     9421 taattaaaaa cctacatatc agtttagtat tcgtgtagat gattatataa atataaaagt
     9481 ataagaaagg tgagtatctg tggttagttc atttttaaat gatatcttta gaacaatttt
     9541 agtaagttgt atttttatag ttattttatt agtctttaga ataactttat ttaagatttt
     9601 cagtaaaaaa tttaattact atatatggct tattgtgatt attaagctat cattaccatt
     9661 tatgaattat acttttatat ttaatgagtc aaaatataat ggtaatatta ataagataag
     9721 tttaggcaat tttaatagta tttctattgt atatagtact gttttagtct ctgtttggat
     9781 tgctattacg atattttatt tagtttatac tttattgaaa tatattaagc ttaaaaatat
     9841 gataaatgat ttgtcttatg atgttgagga tgataagata aaaaatcttt atgaaaattt
     9901 attaaaagag cttaagataa ataaaaaaat acggcttaaa tattcatatg aagttgaaag
     9961 tccagtattt tttaattcgt gtgtcatttt accaccaagt gattatacat taaaagagtt
    10021 aaactggata tttaggcatg agttgatgca ttttaagagt aaagaccttt atattaagta
    10081 tttggtgata tttttaaaat gtgtttattg gtttaatcct tttgtgtata taatggagaa
    10141 gtttatagat ttggattgtg aattttattg tgatgaaaga gttttgaaga attgtacgat
    10201 agaagagaag caggattatg cattgacaat aattagtgca atgagaaaag gttcaaatag
    10261 ttcaaataaa tttgtggctg gattacataa ggagtcagat attaaaaaaa gagtgtttag
    10321 tatgtttaat gaaaagtaca gaaatggtat tttgatagca gttattttat gtttattatc
    10381 ttctattaca tattttaaaa ttgatttaat tactactaaa tcaatatttt atccactttc
    10441 aaaacaaaca cctattgaaa acagaactaa ttcaaaacct acatatcaga ttaatgttta
    10501 tatagataat taaaaaatac aaagtttaac aagagattaa tataaataat tttaataagt
    10561 atctttcaca tatgttaaat ttactttaat tatattaaag ccaatgaatt attctttaga
    10621 agctccaatg ccagtaaata caggtaaaga aaaatatttt actacaattg cagattaaaa
    10681 agtgttctaa tatttaaaga aatacacaag tgtaagaagg gtaaaaatct gttataatac
    10741 attaaaaact aaatttagaa aaatttacat aattagaaaa atgatttgat aaaaaataaa
    10801 gttaaatatt aactaagtaa atttgaaaaa gaaaaaatat aaagtacaaa tttaaaagga
    10861 ggtaactaat gaaaaagaag ttggaagata tgactttaga ggagttatgg aacttgtttc
    10921 ctatattttt gactactcac aaagaatgtt ggaaaaattg gtttaaagaa gaacaatata
    10981 gactaactgg aatattgcca atgcaaaaaa ttgttagaat aagtcatatt ggaagtactt
    11041 ctgtgagtac gatttatgcc aagcccattg ttgacatact gattgagata attcctgatt
    11101 ataatatgaa agatattaaa gatttgattg agtcgaatgg atatatttgt atgtctgaga
    11161 gcagtagtag gctttcactt aataaagggt atacagtaga tgggtttgaa gaacgagttt
    11221 ttcatttgca tataaggtat gtaggtgata atgatgagtt gttttttaga gattacttaa
    11281 ttgaccaccc agatgttgca aaggagtatg aaaaacttaa gttggacctt tggaagaagt
    11341 atgagcataa tcgtgatgct tatacagata gtaaactaga ttttgttagg gagtttactg
    11401 aaaaggcaaa ggtgttgtat tttaatagat ataattagaa ataggtacaa ttagatattt
    11461 atcttatata tcgtattata ttttttaaga aagtaattaa tagaaaaagg ggttgtttca
    11521 aaatggagag ataaatccct ttttcattgt tgaaaggata aaaatatatt tgatttcatg
    11581 taaaaaagag tgcctcatca actaaaaagt ttattttgag acacacacat ttttactaag
    11641 gtaaataaaa tatcacattt gattaaattt taagttaatg aatatatgtg taaatattta
    11701 attataagtt attataattt tacatagtat caaacaatct aatttgtatt atataagata
    11761 atctaatttt agactaaaat aatctaatat aaagaaataa agaaaattca ttctcagtat
    11821 aagagatatc caattttcca ttgtacttat taacaatttc tctaatattc tcaataccat
    11881 atccgtgaga attactatct tttttatctg aaataaatag ttcttgtttt ctattgatag
    11941 gatttacctt gttgtttgtc aataaatagc taaaatactc cttctcacaa cgtgccttta
    12001 ataccacttt tctatttgac aaatcaggaa tcttcaaaga tgcttctata gcgttatcta
    12061 aagaatttga aaaaatagta cataaatcta taggttctat acccaagtga ttgtctatat
    12121 ctatattaaa gtgcacatca acaccatttt cttccaaact tgtatgacga agatttaaaa
    12181 ttgagttaag agcttggttt ttgcaaaact gtttgccact aagagtacct atacctattt
    12241 ttaactgatt aaaatacttt ttagctccag caatatcttc aatttctaaa tacgattcaa
    12301 tcatttgaaa gtgattattc atatcatgtc gcattttgcg tatttgaatt tgttgatttt
    12361 ctagtaagtt atagtatttt tcttttagat gatttgcttg tttttctatc ttcatcttta
    12421 cattttcaat caatggctgt aaaagaagta tcacaccgat atttgccaga attgctgaca
    12481 agatgattag ataagtgcca taaggtaagt tgcctaagat aatattctta ttactaccca
    12541 tagggattaa attagtaggt ggcagaataa tagcaattaa tattgataaa aatgaagata
    12601 tacataccat atcaataaaa atccatattt tatttgataa gtattgtttt atattgacca
    12661 atctattctt taggaaaata tgtattaaaa accaaaataa taatttaatt agagcagaaa
    12721 ttatctggac tattgaaagt attaaaatat actcttttga gtgtataagt tcctcatata
    12781 tagggttgtt tataaaaaga aaattaagcc caattataat aggataaaat atcatgacaa
    12841 ctgatattat aggaattaat ttgcttttgc tacatattat aagtagtata aaatatccga
    12901 atagtgtaaa caaaatattt acggggtcgg aaagccatat tatcatttga ctaataatag
    12961 agtagcccaa aatcaatatg attttgaaaa tccaatgatt cctaaagtct ataaaatagg
    13021 aagtcaacgt ataaagaaaa aaggctatga aaatattgat taatattaga ataaaattta
    13081 tgcttgtaga gattaaagaa taagtgccca tttaatatac ctttctatta attaagattc
    13141 tggaaactaa gttttaaaaa attaaatctt aaaaattaaa attctggaaa ttaaatttca
    13201 aagaattgat tttgaaaaat aaaattctag agattgaact tcaaaaaatt gagttttaaa
    13261 agattgaaat tttaaaaatt aagtttcaaa aaattaaatt tgaaaaaaaa ctctaaaaac
    13321 taagcttcaa aaaattaaat tttgaaagat tgaaattcta gaaattaatc ttcaaaaaat
    13381 taaatttgaa aaaacaaaac tctaaaaact aagctttaaa aagttaagtt tcaaaaataa
    13441 aattctagag attgaacttc aaaaattaag gttctaacat ataccttgta aattttccta
    13501 caacctcttg atatttttta cggcttatag gaattttttc attctgaact ttggcataag
    13561 tttgagttat actatctaca tggtttagat tgacaatata cctttgatgc gtacgtataa
    13621 aattagaggg aagaacagtt tccatatcat ttaattttcc gtagtattct aatgttttat
    13681 caaaggtaac tacagttaat tttcttagct cacttttaaa gtagaggata tcttttgtat
    13741 taattttaca aagattagtt ccaatctgaa ctgtaataaa gttttctttc cattgttcta
    13801 atttttttag tgcttctttt aatactttac atattttttc agttttataa ggttttaaga
    13861 tgtagttcaa agctcctaca tcatatccat gaaatacata gtcagaaaaa cctgtgacaa
    13921 agatgattat agtatccttt gaatgttttc gtatagtttt tgctatgtca acgccattac
    13981 tttttccaag ttcaatatct aaaaaaataa tatctatgta ataattttcg tgttctagat
    14041 tgtatactag actttctcca tttgaatatt cttttatttg gtaggggtag ccccacaaat
    14101 ccatttctct tgtaatgatt ttattcagca tgtttctttg ttgtttttca tcatcacaaa
    14161 tacaaatcga agtcaatttt ttatctcctt tcaaatttta tatttatata gttaagtata
    14221 taatatattt tgctacctag atatataaaa tgaaataaca attatatcca gatagcaaaa
    14281 taaaattttt tatattagta tacttacact taaaact
//
LOCUS       DAMJWS010000097        14297 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMJWS010000097 DAMJWS010000000
VERSION     DAMJWS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.101"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            122..1750
                     /gene="aspD"
                     /locus_tag="PC399_003168"
     CDS             122..1750
                     /gene="aspD"
                     /locus_tag="PC399_003168"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF2594007.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1871..2866
                     /locus_tag="PC399_003169"
     CDS             1871..2866
                     /locus_tag="PC399_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF2594008.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3055..3438)
                     /locus_tag="PC399_003170"
                     /pseudo
     CDS             complement(3055..3438)
                     /locus_tag="PC399_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3664..3761
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3903..4260
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3903..3931
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(4546..4704)
                     /locus_tag="PC399_003171"
     CDS             complement(4546..4704)
                     /locus_tag="PC399_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594009.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5063..6433)
                     /locus_tag="PC399_003172"
     CDS             complement(5063..6433)
                     /locus_tag="PC399_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HDF2594010.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6716..6814
                     /locus_tag="PC399_003173"
                     /pseudo
     CDS             6716..6814
                     /locus_tag="PC399_003173"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            6833..7254
                     /locus_tag="PC399_003174"
                     /pseudo
     CDS             6833..7254
                     /locus_tag="PC399_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7485..7757
                     /locus_tag="PC399_003175"
     CDS             7485..7757
                     /locus_tag="PC399_003175"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2594011.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(8075..8743)
                     /locus_tag="PC399_003176"
     CDS             complement(8075..8743)
                     /locus_tag="PC399_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2594012.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9579..11999)
                     /locus_tag="PC399_003177"
     CDS             complement(9579..11999)
                     /locus_tag="PC399_003177"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF2594013.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12353..12703)
                     /gene="rsfS"
                     /locus_tag="PC399_003178"
     CDS             complement(12353..12703)
                     /gene="rsfS"
                     /locus_tag="PC399_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF2594014.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(12820..13392)
                     /gene="yqeK"
                     /locus_tag="PC399_003179"
     CDS             complement(12820..13392)
                     /gene="yqeK"
                     /locus_tag="PC399_003179"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF2594015.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13393..14082)
                     /locus_tag="PC399_003180"
     CDS             complement(13393..14082)
                     /locus_tag="PC399_003180"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF2594016.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4831 a         2309 c         1808 g         5349 t
ORIGIN      
        1 ttattttaat ttcatacttt actttttcta taaacctatt ataattaaag tataatcatt
       61 tttgcaactt gtttatttta ttataaaaat gtgaaactta ttacataagg aggaatttaa
      121 catgaaaaat catacttata aagaaatcaa aaacatatat ggaaaaataa gcccttttga
      181 atttaaggac aaattaatag atattgcaaa atacactgca aaagaaaata acagagaatt
      241 acttgatgca ggtcgtggaa atccaaactg gacttgttca actgctagag aagccttttt
      301 tacttttggt catttcgcta ttactgaaac tcgttctaat tgggatttag gtcatttggc
      361 tggaatgcct caaaaaaagg gtattaaaga aagatttttt aaatttataa atgaaaatat
      421 agatatgcct ggagcatatt tagctagaga tattataaac tttggtataa atgaacttgg
      481 atttgatgga gatgaatttg ttcatgaact agctgatgga attattggtg ataactatcc
      541 acttccagat agaatgttac ctcatatgga aaaaatagta cacgattacc tagttcaaga
      601 aatgaaatat gatatatcag gtaaatatgg tgatgtagaa atttttgctg ttgaaggtgc
      661 tacagctgct atgtgctata tatttgattc attaatggca aatgaacttc taaaaaaagg
      721 agataccata gccttaatga ctccaatatt tacaccttat cttgaaatac ctaaccttcc
      781 tcgttacgac tttaaagtgg taaatataaa tgctaatgaa gttgatgaaa aaggtgctca
      841 tacatggcaa tatacaaaaa aagaattaga gaaacttcgt gacaaatcta ttaaagcttt
      901 attcgttgtt aatcctaata atcctgcttc tatagctatg gatgaaacat cttgtaataa
      961 tctaatagat gtaattgaaa attataataa agatttaatg ataatatcag atgatgtata
     1021 tggaactttt gtagaagatt tttcatcctt aatgtcaaaa ctaccttaca acactgttgg
     1081 agtatattct tattcaaaat attttggagt tacaggatgg agacttggta catttgccct
     1141 tcataagaaa aatgtttttg ataagaaaat aaatgattta actggagaat taaaaaaatc
     1201 agtagataag cgttacagtg atatgagtct taacccttct agtctttcct ttatggaaag
     1261 agttgttgct gatagcagac ttgttgctct caaccatact gctggtcttt ctactccaca
     1321 gcaagtacaa atggcgtttt tctctgcttt tgcattgatt gacaaagtag atgcctacaa
     1381 aaaacttaat atgagtatct gccatagacg tcaaaaacta ttatttgaag ctttagagct
     1441 tccaattaat gaaaacaaaa acaatgctgc atattacact caatttgata ttgaagaatg
     1501 ggctaaatta aactacggag aagacttatt taaatttata agtaaaactg catctccagt
     1561 agatgttctt tataaattag ctaatgacta ttcagtagtt ttacttagtg gaaatggatt
     1621 ctatggccct gaatggtcaa ttagaatatc tctagcaaat ctatatgatg aagcttatac
     1681 taagatagga aaagccataa gagagatatt aaatggttat atcgttgaat ggcaaaaaat
     1741 taagaaataa ttatattaca ctgaaatatc taaattatat gaataaaaga gtttatgtag
     1801 accttaacta ctttttttga tataatccta tcggaattat aaattttatt tatgaaaggc
     1861 agagaatagt atgagtatta agaaaaaagt tgccatagtt tttactggtg gaactatatc
     1921 tatgacagta gatgataaag tcggagcagc tataccaact ttatcaggtg aacaaatact
     1981 atcaatggtt accaacattg ataaagttgc agatgttgaa gtttttaatt ttgatgaaat
     2041 tccaggacct cacataacac cttatagaat gatggaattg aaaaattatg taaatgattt
     2101 attagcaaga aaagatatta caggtgttgt aataacacat ggtacagata gtttagagga
     2161 aacagcttat tttctagacc ttacaattga caacatcaaa cctgtaatag tcacaggtgc
     2221 tatgagaagt agttctgaac ttggatatga tggttcaagt aatttatctg catcagtttg
     2281 tactgcagta tccaaagatg ctatgggaaa aggagtcctt gtagttttaa acaatgaagt
     2341 attattagct tctgaagcta ctaaaacaaa tactctttct ctaaatacat ttaaatctct
     2401 aactagtggc ccattaggaa taattgactg caacgaactt gtccttacaa gagatattgt
     2461 aaatagaaca attatagata ctgataaggt agaatctaaa gttgccttat ttaaagcata
     2521 tgtaggagaa aatgctgact ttataagatt tgctgttgat agtggttata aaggtatagt
     2581 tattgaggca atgggcagag gtaatattcc tcctcaaatg ctttctggag ttgaatatgc
     2641 tagagaaaaa ggacttcctg tagttatagt ttctagatgt cattcaggta gagtattcga
     2701 tagttatggt tactttggtt ctggtagaga tttaaaaaat cttggttgta tatttggtgg
     2761 ggacttacca ggtcaaaaag ctagaataaa acttatctta gcacttggaa aaactgataa
     2821 ccttgatgaa atcaaagact tctttgaaaa aggaatctat tgttaattct aataacttct
     2881 atttaattat ttattgatat ttcaacattt ttaaatcaaa atctaatact tatgtcagtt
     2941 aaatgtcagc aaaatatttt atccacaatt tttacacaaa ttttccacaa cttatcccca
     3001 atattatcca caagaaaaaa ggtagcactt atattagtag ctacctttta tttattaatc
     3061 ttcatctttc cagcttttta tccaatttcc tatttctatt attttttctt cagttatttc
     3121 ttctccatca tgtaattttg aaattagttc tgataatgga ttgttatgta gacctccaaa
     3181 aattcttttt ccctctattt tgagatattc tttctctttt attaatactc tatagtgtac
     3241 atgtttccca attctctgcg atatgaaaaa acctttttta gttaatctag ctaaaagtgt
     3301 aattgtagtt gtatgcttcc aagcatatgt tttttctatt taatctgcaa catcttttga
     3361 tattacagtt ttataaccag tattccatat gtatttcata atttttaatt ctgcatctgg
     3421 taatttttta ttcaacataa tatattcctt ctttcaattt atgtcacttc ctcaaactta
     3481 tctagcttaa attaataata ggagcttttt atcacaaaaa ctacaggtaa atttttctat
     3541 atattctacg tatttagtct gtttttaagt gtgaaattac gttcatacct ataaatttat
     3601 tcaattttca aacaatcttg ttgaataata actaattcta ttgtattatt tagttataga
     3661 ataaaactaa atcggcaaaa ctagagaaat ctagtgacgc aaagctatag ggactaagac
     3721 ttatataaat atcttatgag ttatgttagc cagttgccaa agagatattg ttctttttgt
     3781 ttttatgaaa gttttttagg gggataataa tttatatgtt tagagataaa atggataaat
     3841 gtacacatat attgactgct tatattagca attcgtatga ttattgtaat tttttggata
     3901 caatttacat tccatatagt taatataaaa ctcaattaaa ttatgacctt ggtttagata
     3961 gttcattatt tacattccat atagttaata taaaaccaga aggaatatct ggagttattg
     4021 gagggtctat tggatttaca ttccatatag ttaatataaa acatttacta agtgtagata
     4081 taattataaa tacgatttaa tttacattcc atatagttaa tataaaacct gtagacttca
     4141 accctacaat gcttgttgga agtgtattta cattccatat agttaatata aaacttttgt
     4201 aaaagtttag aagtttatat ataaaaaaaa tatttacatt ccatatagtt aatataaaac
     4261 cccaaaataa attgagtatt tccaatgcct acacatatac aaatctctca aatttgcagt
     4321 gaaccatgag tagtgcaatt gataacactt atcacaagtc ctcaatgcct tatatttcaa
     4381 ctgttaagcc ttaatttctt caaaaaatcg aacactgcaa aatttctatg actttattat
     4441 atcataaaaa tattattttt gaatatctgt accaatttgt gatataataa aagcaagaag
     4501 aactacaatc tattttgctg tagagtggag ttcataattt agaaattact tagttttatg
     4561 gaatctgatt tttaaatcaa attcccaacc actcttagtt gccgctttga gtggtttttt
     4621 acgctttcta aatagtttgc tagctatata aactattaag ctagcagata gacttgctag
     4681 tatgccttgt aaaaaattat ccatgtatat tcacctccct tcttagcgtc gggaggataa
     4741 ttttttgata catgaactcc actctataaa ttgtagatta catcttcttg ctaaaaatat
     4801 tataacatat aattcttaca tattttacct atttattact ttttacatta taataatatt
     4861 cttttaatca aaaaaacacc tactattaaa gtaaatgttt ttaagtattt ttaattttaa
     4921 aatccatata gttcatatga aactaaatat aataactcaa catatgagca atatcgagac
     4981 atttacatac cacatagtta atataaaacg aaaataaaaa aattaaactt ataaaaccct
     5041 tattaattta catacccact agttatttat attcaaaaaa atcttctcca tctgggttat
     5101 aaaataatat tctacttgcc aatccttgtt catatttata attttcttta tcacctacat
     5161 aagaaaatgt taatgtcatg cttcttgatg gatctaatga aattatatct aaataaatag
     5221 tacatttttc tcctggcttt aatacaccga aactattaaa atccattttt attttagcat
     5281 attgccccat atataatgtt gaattagctt ctccataaac ttttaatttt gttacatcta
     5341 aattttgtgt acttgtattt tctatattta taggaactgt tgggtcttta tatttttcag
     5401 tataatattc aaggttatca tcataacatt caagataact tcctttatct tgccaaatta
     5461 tgtgctttga atcaaaatca atctttaatc cacatattat atttttattt gaagacggaa
     5521 gactaatatt attgatggta ttttcacact ctagcataat tttatcactt agatttccta
     5581 tcccagtcac ttttgcagca tttttaagta tatctttatt acttccaata tcaactagta
     5641 caataggatt atttttagcc attggtgaag caactaaagc ataaactaaa tcagtaccac
     5701 tagctatata aaattctttt gcgccattat agaacttatt tactattttt ttattagtat
     5761 catatctatc tactccacct aatctagttg aattagtatc attaactagt ttgtcactca
     5821 ttgatgaagt accacctatt acataacttt ctacaccagt tgtattaaat ggaacacttt
     5881 ttccatcagt taatactata gctgctttat ctctaactgc tacaggtgca gcactcattg
     5941 catcaggctc tcctttaaaa gcattagtca atataacttt atttactttg ttaattgagt
     6001 ttatttcttt tgcaacatta tagcttgtct taattctatc actaccttga agtcttttag
     6061 cctctattcc tttatctttt agaagagttt ctgttgcttt atcaatagaa ctttctccac
     6121 ctattatgta taccttttta gctttctcta ctcttttaag agttgcatta ggtatgttat
     6181 ttttcttagt taaaaaaatt ggtgcatttg ttgctcctgc aagtccactc gcacttaatc
     6241 catcagccat tgtgctgtct gcattaatta atatagctgt agtataattt tgttcatcag
     6301 ctataagagc agccgtttca tatttatctt ttccttgaat agtataaatt gtatcaagtg
     6361 cattagctga tataggacca gcagcaaaca ttgacacggc taaccctagt gataataatt
     6421 tctttaattt cacaatatct ccccctttta tatatgaaat actatatatc ttacataatt
     6481 ctacattaaa ttttcttttc ctcctttaac gatatttatc ttcctgttaa tttaagtgta
     6541 tacaaatacg tgttataatg taactgtaaa gaattaaaaa gataatgaca taattagagc
     6601 agtccaactg ttcttttata aaactaactt ataaagggag cgtacatata tgaatatcaa
     6661 atcagctttt ataagaaaaa gatgggaaaa attctatgta tatgtggaat aagaaactgg
     6721 caaaaagaaa caaaaaagtt atggaagcta taaaaagaaa aaggatgctg aaaaacattt
     6781 aattgaaata aagtctacta taaataataa taagtttgtt gctccaaaag gtatgacatt
     6841 tgtagaaaga tgttataaat atatagacat gaataaaaat aattggtctc catatactgt
     6901 tacaaataca aaatcttggg ttaaaaatta catagaacct tttttaagaa tctaaaactc
     6961 acagaagtaa atcctagttc agtacaagct tttgtaaatt ataactttaa taattctacc
     7021 tttactagtg ctaaagtaag gtatagcttt ctaagttctg taataaggga agcgtataga
     7081 ttaagagaaa tacaagaaaa ttcatgtgat tttataaaac ttccttctaa agaaaaatat
     7141 tctacaaatg aaatatacaa tagagaagaa gtattattgc taatagaaaa gttacttggt
     7201 aataatatag aaatacctat tcttttaatg ttacttctag gtttaagaat gtgagaagct
     7261 attgggctta ggtggtcaga tgttgactta gataacaatc tgataaatgt taatcaaatt
     7321 cttgtctatt ctaatggcaa aatatctttt aaagagccaa aaacaccaaa atcaaaaata
     7381 gctttatcag ctccaaaaga attaattgaa aaattgaaaa ttgaaaaact aagacaaagc
     7441 aaattaaaat tgtaaggtat cctggaaaac agaaataact tagtttgtct aaacaccaat
     7501 ttaaatccgt ttctaccttc ttcactaaga aaaacgtttc acaattttac aaaaaaaata
     7561 atttaaaaaa gattagaata catgatttaa gatatacaag tgccactctg ctgcttttag
     7621 ggggaactaa tatgaaagtt gtttctgaaa gattgggtca tacagatata aaaataacta
     7681 tgaatagata ttctcatgta cttaaagaaa tggacaaaga agcttccgac aatctaagcg
     7741 aacttctatt taaatagtaa aaactgactg tcagttaaat gtcagttaaa cctaaaaaaa
     7801 taggttttgt cagttgatta tcagtcaatt attttataat agtttcactt aaaaacgtct
     7861 atatctacac attttactat ttgtttatgc ttattaatat ctatctgtta ttattaattc
     7921 taatagcttc tatttaatta tttagttcag tttacaggaa actacacaca aaccataaaa
     7981 taggatgttg acaatataac agatagtatg ctcctgtaaa aggtaacata gtatctgtta
     8041 tattgtctgg attcccaaat tagctatttg gaacttaact aaaaaatcct aatataaggg
     8101 caactattat aatggcaatc ggaatacata ctgtgtaatc ctgttccata gagtcatcat
     8161 ctgaatgtag ttttacaatt cgcaatttag caatcagttt gaagcagaaa tagcctatta
     8221 ttgttccaat tacgttcgtg agtaagtcgt ctatatcggt tgctctgtgt aacgtaaaaa
     8281 gctgaatagt ttcgataaaa agagataatc caaaaccaat caagaatgta tttctcaaac
     8341 gttcaaaggt tttgcttata agcggacata aaaaacctaa tgggacaaaa agtaaaatat
     8401 tcaaaataaa actcaaacta aatccatcac taaaaggaat aagatttatg tttggattaa
     8461 agaacgtttc ccctaactgt gacagtctta tatattcacc gaatgtaggt ataccaacaa
     8521 tatgcgatag tgttaaacat aagtagtaat acattgccag tgataaaata gatgttttga
     8581 cctttggaat atcactattt ttcttgaata ttacaactaa gaaaatacca atcaagataa
     8641 ttcccaacaa gaaaatagga ttaattaaca ataaatcttt gatagcttca aacagactga
     8701 gttgaatttc tacacctgat tgattatttg ttacagtctc catttgtaca tacctcctca
     8761 taacatttca attatttcta aaaaacaaaa tagaagataa atcttaaaat catcctatca
     8821 aaatttttaa atttgccttt atcagtttat accaccagtt aattcctgtc ttacttctgt
     8881 atggtattct tgggattctt tcatcaaatt agacaaaaaa tggttaccat atcaataaac
     8941 tggttacgct ccatatctcc cgaagcaacc atcccctgag aataaatccg tatcaggaat
     9001 ctccacatct actgcaccta tcacaagtta tttgataaca tcctcagaca atcccaactt
     9061 ggatatatcg acaacaccca ttagaatacc actgctgacc atacttgttt tcctgctgat
     9121 aattttaatc aactgggaag ttgaaacgct atctttttta caagttcctt tttagaaaca
     9181 tggtagctat tatatgaatc tgaatttcgt tgtctgggtg tttcatataa agcatagata
     9241 tgcacctttt ttgcctgttt tcattatata gaaaaaaatt tcaaaataca ataattatat
     9301 ttttttcgtt taagcttaga gcagttcctt gacgaacaac actttggaaa ctgaatgatt
     9361 tacataccac ttaatacatt aactcgattt aacattaatt ataataatat ttaaaacaac
     9421 aactagggta tataagaatg aactttttag ttctttaatc atttttttgt ataaaatcaa
     9481 atctattttt ataatttcaa caatgaaaaa aggcttatct ctattttgag acagcctcat
     9541 ttattttata aatttatatt tttgttcata tttttgagtt atttaacaac tatatttact
     9601 aattttttag gtacagctac aactttaact atagttttac cttcaactaa tccttttatt
     9661 ttttcatctt ctaaagctac cttttccatc tcatctttag atatatttga gttaacagtt
     9721 aattttcctc taacttttcc attaacttgt acaacaactt ctatttcatc ttttactaaa
     9781 gctttttcgt catatttagg ccagtctata tcaaatacac ttccttcttt acctatcata
     9841 gtccatagtt cttcacctaa atgaggggca aatggtgcaa ttatagttac tatagtttgt
     9901 acaccttctt ttataactgc ttcgtttata ttatctaact ctttatattt atacatatca
     9961 tttattaatt ccataagagc tgatatagca gtattaaatc cgaatttttc acttaaatca
    10021 gcagtaactt ttttaagtgt agagtgtata gtatatctca tatccttatc ttgggaattt
    10081 aattctccaa actctacatc ctttttaacc acatctgcta actcgtcaac caatctataa
    10141 accctattta agaatctaaa gcatccatca acaccttgct ctgaccagtc caaatctctt
    10201 tctggtggtg ctgcaaataa tacgaaaagt cttgcagtat ctgctccata ctcatctatt
    10261 atatccatag gagatactgt attcccttta gacttactca ttttagaacc atccataagt
    10321 accattcctt gagtaagtaa gtttttaaat ggctcattga agtctaccat tcccatactc
    10381 ttaaatgcct ttacaaacca tcttgcataa agtaaatgca ttatagcatg ttctactcca
    10441 cctatatatt ggtctactgg atgccatcta ttaactaatt ccttactaaa tggttcattt
    10501 tcattattgc tatccacata tcttaagaaa taccaagaag aatcaacaaa tgtatccata
    10561 gtatcaactt cccttctagc tggttttcca cagtgaggac aagttgttga cataaattgc
    10621 tttgaagtcg taagtggaga ttcccctttt ccagtaaatt caacatctgt tggtaaaaga
    10681 actggtaaat cttccttttt aactggaact attccacatt catcacaata aaccacaggt
    10741 ataggacaac cccagtatct ttgtcttgaa actaaccagt ctctaagtct ataattaatt
    10801 gttctctttc ctctaccttc tttttctaac ttatcgatta ttccttcaaa tccttctgaa
    10861 gcatctaatc cattaaattc ttctgaattt atcattttgt tatcttctgt tattacatca
    10921 attatgtcta aattatattt ttcagcaaaa tctctatctc tttcatcatg tgctggaact
    10981 gccattatag cacccgttcc atactcaact aatacatagt tagctatcca tagaggaact
    11041 ttcttgccat ttaaagggtt tataacatat ctacctataa acattccttc tttttctaca
    11101 tcagctgctg ttctttctat ttctgtcata gtatgcattt tttgaacaaa agcttcaaca
    11161 tctgcttcat attcagtccc tgcaaccaat tctttaacta attcatgctc tggagcaagt
    11221 accatataag taactccata agttgtgtct ggtctagttg taaatactgt catagattta
    11281 tcagttccat ctatatcaaa aactaaatct gctcctgtac ttttacctat ccaatttttt
    11341 tgcattgttt taactttttc tggccatcca tctaatgtat ctaagtcatt aagtaattct
    11401 tctgcaaact cagtagtctt aaaataccat tgttctaaat ctttctttaa tactgttgct
    11461 ttacatctct cacatgctcc ttgaacaact tgctcatttg caagaacagt ctcacaagaa
    11521 ggacaccagt taacatatga ttttttctta tatgcaagcc catgctctaa aaacttcaaa
    11581 aatatttctt gtgtaaatct atagtattct ggagttgaag ttgcaacttc tttatcccaa
    11641 tcataactaa tccctaataa atttaattgc tctttcattt cttctatatt ttccattgtc
    11701 catttatgcg gatgtattcc atgttttata gctgcatttt cagctggtaa tccaaaagaa
    11761 tcccatccca ttggatgaag tacattatat ccttccattt ttttgaatct tgcgacaaca
    11821 tcacctatag aataatttct aacatgtccc atgtgtattt ttccagatgg ataagggaac
    11881 atttctaatg tgtaataatt aggtttttca gtttgtgcag tatccatttt atactgatta
    11941 ttatctttcc agattttttg ccatttcgat tctacttctt taaaattata tacgctcata
    12001 ttttgcctcc tatttttaat tgtgtataac taccttattt agtattattt acttataatt
    12061 tccaaataat tctttgaaaa taaaaaatcc tcgtcactaa acttacgttt agggacgagg
    12121 ataaatcttc gcggtaccac cctaattagc ctactacata tatagactct cttagatagc
    12181 ttttaatcca gccatggaga atagatttta atctagtaag ttcaaagtta tataatatta
    12241 gttctcacct tccactaact ctctgaaaaa atataatctt ttactgctct agtctattca
    12301 atttaatata atttttgaaa ttttctaatt ataataacat atattatcat gtttatgcta
    12361 atgtgtcaat atctatataa ggtgcatctt tccataattt ttctaaatta taaaaccctc
    12421 tattttcctc attaaataca tgaaccatta tgtctccata atcaaggagt acccaagttt
    12481 gagttccttg tccttcttta cctcttggct ctaatcctag ttttgctaat tcatcttcca
    12541 cattgtcagc aatagctttt acctgtctct gagaagatgc tgtagtaata ataaaataat
    12601 cacacaaact agaaactttt cctatattta ttataactgt atcttgacct agtttatctt
    12661 ctatggcatc atatgctatt tttgtcattt gttccactgt catataaaaa ttcctcactt
    12721 tcctaattaa aatacactta gttattgtaa catattaata cgattttgta acatagtcaa
    12781 atttaaattt attaatatgt ttgcctttat atagcttttt tataataact tttctttaat
    12841 tagatagttt cttgccataa ctgttcttgg atgtatctct tcttttttat tgatgacaaa
    12901 cattaaagta ttattaaaag aagttaaaag tgcttcatca agcttccctc catatgctaa
    12961 ttctcttaat gtatctacaa ctgggaattt tcttccttct tcaattaaat cagctatgta
    13021 aattattttt tctaaaagag acatgtcctc tctccctgtt gtatgatatt tgattgcatt
    13081 tataatatct tcatcttcta cttcaaattc atactcgcat atgtacgccc caattacgct
    13141 atgagacaag gaacgatttc catcctctat tgggtctaaa tatatttcat acttatttac
    13201 ataatcctct atttccttgt cacttaaata ctttgcacaa tcatgaatca ttcctgctaa
    13261 gtaagctttt tctttgtcac atccataaat ctcacataat ttttcagcac attttgcaac
    13321 attttttgaa tggctaagcc tccccactgg tagcatttga tttagtcttt ggttaatatt
    13381 ttctaagttc atttaatcat ctccatattg atatagttta ttttcatata aataattttc
    13441 tacattttcg ggtactaagt aacgtattga cttgccttca tttagctgtt cccttatata
    13501 tgtggacgat atatccagtg atggaacata aactgttata atgtcagcat tatacttctt
    13561 agttagtttt tctatagtct cttgtgacct taacaaacta attcctggtc tagttgcggc
    13621 tataaaagta gctaactcaa agtttttctt tacatctttc cactcttcaa cactacaaat
    13681 tgcatctgca cctgttataa aataaatgtc tgcatttttg tattttttct ttaagtattt
    13741 taatgtatct attgtataag tccttttctt tctttcgatt tctacttttg aaactaaaaa
    13801 atcttcgttg ctttcagttg ctagaagtgt catattatat ctatcatact tatctgtagt
    13861 gatacataat ttatgtggtg gatttcctga tggtataaag attattttgt caatgtcata
    13921 cttgtctcta ataaattctg ctgttgctaa atgagcatag tgtatagggt caaaagtacc
    13981 tcccaatata cctattttga ttttgccttt atggcgatta aatttctcta gtttttcagt
    14041 atttttcaac tctgccatct taactagatt ttcacttctc atattttgtc tccttttaat
    14101 gttataatac tattgtacca ttatttttta tttcttaaag cttataattt gtttttttgg
    14161 gtatataatt atatttatat gctaaaaaac acttaaaaaa ataattattt aacatgattc
    14221 tactttactt tttaataaaa ataacttttt actattttgt cttaattttg ataaaatata
    14281 acatacaaat ataagtt
//
LOCUS       DAMJWS010000098        14018 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMJWS010000098 DAMJWS010000000
VERSION     DAMJWS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14018
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.102"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            419..1312
                     /locus_tag="PC399_003181"
     CDS             419..1312
                     /locus_tag="PC399_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF2594017.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1312..2706
                     /gene="gltA"
                     /locus_tag="PC399_003182"
     CDS             1312..2706
                     /gene="gltA"
                     /locus_tag="PC399_003182"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF2594018.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3072..5291
                     /locus_tag="PC399_003183"
     CDS             3072..5291
                     /locus_tag="PC399_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2594019.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5438..6184
                     /locus_tag="PC399_003184"
     CDS             5438..6184
                     /locus_tag="PC399_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2594020.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6184..8064
                     /locus_tag="PC399_003185"
     CDS             6184..8064
                     /locus_tag="PC399_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594021.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKNYTRKVWF
                     FD"
     gene            8183..9574
                     /locus_tag="PC399_003186"
     CDS             8183..9574
                     /locus_tag="PC399_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2594022.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9681..10721
                     /locus_tag="PC399_003187"
     CDS             9681..10721
                     /locus_tag="PC399_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2594023.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11210..11863
                     /locus_tag="PC399_003188"
     CDS             11210..11863
                     /locus_tag="PC399_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2594024.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            11939..12148
                     /locus_tag="PC399_003189"
     CDS             11939..12148
                     /locus_tag="PC399_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594025.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12288..12854)
                     /locus_tag="PC399_003190"
     CDS             complement(12288..12854)
                     /locus_tag="PC399_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF2594026.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13015..13668
                     /locus_tag="PC399_003191"
     CDS             13015..13668
                     /locus_tag="PC399_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF2594027.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13826..14002)
                     /locus_tag="PC399_003192"
     CDS             complement(13826..14002)
                     /locus_tag="PC399_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594028.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5488 a         1389 c         2370 g         4771 t
ORIGIN      
        1 ggtattgcaa tagattaaag ccttatacac gtttagtttt taaagaaatt agtgttatta
       61 tgaaaaccta agttaattta ttaaataaat aattgaataa aaaaatttta ttatgtattt
      121 ataaaatttt tgttacaaat ttggcataat atagtatact ttaagtgtgt attaggttaa
      181 atctataaca aattagacgt atgatataga cttagtgttt attggcattt acaagaaatt
      241 tgttaatatc aactatttgt agtttgggca gttataatta ttataggggg gatgtccaat
      301 acaaaacaat caaccaagaa ctaatattat attataaaga gttatagttt tgtgtgttta
      361 tgtttttaat taaatttaaa gtgggaaaaa tatatttgtt agaatggaga atagcgagat
      421 gagtaataaa atagttagta agagacaatt aacagatagt atttatttaa tggaaataga
      481 ggcacctaga gttgcaaaat cttctcaacc aggtcaattt ataataatta aaaatgatga
      541 aaaaggagag aggattcctc ttacaatagc tgactatgat agagaaaaag gaacagtaac
      601 tattgtattc caaacagttg gtgcttcaac taaaaagtta gcaatgtttg aagaaaacga
      661 ttttgtaatg gattttgtag ggccattagg acaagcaagt gaatttatac atgaagatat
      721 agaagaatta agaaataaaa agatattatt tgtagctggt ggagttggtt ctgctccagt
      781 ttatccacaa gttaaatggt ttaaagagca tggattagat gtagatgtaa taattggtgc
      841 aagaactaaa gaattaatta tattagaaga tgatatgaaa aaagtagcta aaaatgtata
      901 tgtatcaaca gatgatggta catatggatt taatggaaga gttacagacc tattaaaaga
      961 tttagttgat aatcaaggta aaaaatacga ccaagcaata gtaattggac caatgataat
     1021 gatgaaattt atgtgccaat taactaaaga actaaatata cctactatag taagtttaaa
     1081 tacaataatg atagatggaa caggtatgtg tggtggttgt agagttagtg ttggaaatga
     1141 aactaagttt gcatgtgtag atggtcctga atttgatggt catttagttg attttgatca
     1201 agcaatgaga agacagtcta tgtacaagac tcaagaggga agagctatgc taaaacttga
     1261 agaaggcgat agtcatcatc atagtaattg tggatgtgga ggtaacaaat aatggatgct
     1321 aaaaaagtaa aagtaccagt tagagaacaa gaacctgctg tgagagcaac aaattttgat
     1381 gaagtatgct tagggtataa taaagaagaa gctatggcag aagctaatag atgtttggct
     1441 tgtaaaaaac caaaatgtgt gggtggatgt ccagtaggaa tcgacatacc agggtttata
     1501 acaaaaatta aagaagatga tatagaaggt gctgctaaag taatagctaa gagcagttca
     1561 ttaccagctg tttgtggtag agtatgccca caagaaagtc aatgtgaagg tgtatgtata
     1621 cttggaataa aatcagatgc tgtatcaata ggtaaattag aaagatttgt agcagattgg
     1681 tcaaaagaaa atgacataaa tttatctgat acagagccta agaaaaatca aaaagtagca
     1741 gtaataggaa gtggtccagc aggtctagct tgtgctggtg atttagctaa aaaaggctat
     1801 gatgtaacaa tctttgaagc aatgcatgag ccaggtggag ttttaactta cggaatacct
     1861 gagtttagac ttccaaaaca agctgttgtt caacctgaaa tagacaatat aagaaaatta
     1921 ggtgtaaaaa tagaaactaa tgtaattgtt ggtaaaacaa ttacagtaga tgaacttata
     1981 gaagatgaag gatttgaagc tatattcata ggttcaggag ctggtcttcc aatgtttatg
     2041 aatataccag gggaaaatgc aaatggagta ttttctgcaa atgagttttt aactagagta
     2101 aacttaatga aggcttatag agatgattat gatacaccta ttagttctgg taaaaaagtt
     2161 gctgtagtag gtggtggaaa tgttgctatg gacgctgcta gaactgcatt aagattaggt
     2221 tcagaatcat atatagttta tagaagaagt gaaaaagagc ttccagcaag agctgaagaa
     2281 gttcatcatg caaaagaaga aggcattata tttaatacat taacaaatcc taaggaaatt
     2341 ttagtagatg aaaatggata tgttaaaggt atggtttgta taagaatgga attaggagag
     2401 ccagatgatt ctggaagaag aagacctata gaaatagaag gttctgaatt tgtacttgat
     2461 gtagacacag ttataatgtc acttggaaca agcccaaatc cactaatatc ttctacaaca
     2521 aagagtcttg atataaataa gaagagatgc ttaataacag atgaaaatgg tcagacttct
     2581 aaagagggtg tatttgcagg tggagatgct gttactggag cagcaacagt aatatctgca
     2641 atgggtgctg gtaaaacagc agcagcatca atagatgaat atttaaaagc aaaagttaat
     2701 gcttaatttg taaattaaag agagggtgtc tcaaaatgaa ctttttagtt catgaggcac
     2761 tctttttgta caaaatcaaa tatgttttaa tcatttcaac aatgaaaatt tcattcctgt
     2821 agaaagtcta acttttaggt cttagtacaa tctctatttt gatgtagcca cttttttaga
     2881 tggtttaata atttatcagt tattattgta atatttgata ctaagtaaca taactttgtc
     2941 taactatgat aaaatagtta tacaataaag gtcttgtaag gagtgaataa tgtgtggcta
     3001 ataaaatcag atttgtcaaa tctaacatta aataatagaa aaataaattg tgtaattaat
     3061 ggtattttaa tatgaaaaaa agagaaaaag tatttgatgg gctagttttt aagcatatat
     3121 taatcttatt aatactatta gttttgattg atttaacaag taccatcata tatgcaagta
     3181 actctatgga aattacttct agaaatatga ttgaggcatc aaaaagagaa cttgaaaact
     3241 atttagaagt aaacatatct ttattaaaag ctcttagtca agatgataga ttttctaatg
     3301 gtgaaacatc actaattgaa aagggaaaat tattaagtcc atatcaaaag gaatataatc
     3361 tttttatgat tggtataaca gatacaaaag gaaatgcttc aagtacatat agggaaaatg
     3421 ttaactcaat aaaagataag cctagttttg agaaagcaat taaaacaaag caagtagtag
     3481 tttcagatat tgaagtcagt aatgtaacag gtgacaaagt atttatcata tatgtaccaa
     3541 ttgtaaaaaa taatgagatg attgggacca tttttgcttc tttttatttt caagatgtaa
     3601 ataatttaat aagtagaagc aattttgatg attctattaa atttctaatg atagataaag
     3661 attatactat tatttctcat ccaaacaaaa aatatgtaaa tgataaaagt aagatgcttg
     3721 atttagaagg gaatattata ggaaccacta aaagtgaaat cttaaaaaat ataaataaaa
     3781 aatgtcaagg tgatttttta tcttgggata attggagatt atataatgta aaatatacga
     3841 acataaagtg gactaattgg actttagtat caaaatgtaa tatatttaag aactttaaaa
     3901 atctaatagt taattttatg ataaagttgt atttttatat agtaatattt atgatattgt
     3961 ggaaattaag taatgctaaa ttaatagaac aacttaaaaa attggcttat tatgattctt
     4021 taagtggaat aaaaaataaa gaaaagttta gaaaagattc tatgtatatt ttaaagaatt
     4081 attatcaaga taatttttac ttagtacaat tggatgtcaa taaatttaaa tatataaatg
     4141 agatgtttgg atatgctgaa gggaataaga tattaattca tatatcacag gttttaaata
     4201 ataatacaaa taaatatgaa atttgtgcta ggatggataa tgaccatttt atattgttga
     4261 ttgcttgtaa cactgaagat gaacttttaa atagattatc taaaataaat aaagaaattt
     4321 gtaacttaaa tactactaac tcatctaagt ataaaattgt tatgtcaagt ggaatatata
     4381 agattactaa aaaggatgat attaaaaaga ttgacttgtt aattgataga gcaaatattg
     4441 cagcaaaaag taaaaaggaa aaatatgaac attcatattc attttttaat gaagatacaa
     4501 gaaatcgatt gtacaaggaa aaacgattgg aagataatat gaataaagct ttagaaaaag
     4561 gtgaatttat agtatattat caacctaagt atagtttaga tgatgtaaat gaaattgaag
     4621 gagcagaagc cttgataaga tggaatagtc cagaatttgg gtttatttca cctatagatt
     4681 ttgtaccttt atttgaaaag aatggtttta ttgtaaatat tgatatgttc gtgtttgaag
     4741 aagtatgcaa aacattaaac aagtggataa ataaagggta tactcctgta ccaatctctg
     4801 taaatatgtc tagagttcat ttatataggg ataattttat agaaaatata actgatttga
     4861 taagtaaata caatatatct cctgagttta ttgagcttga actaacagaa agtgttgtgt
     4921 ttgataattt aaatatattg attgatataa tgaaaaagat taaagagata ggattcctta
     4981 tatcaatgga tgattttggt tcgggatatt catctttaaa tctgttaaaa gacctttctt
     5041 ttgatatttt aaaattagat agaggatttt taatcgaaac aacagataca aaacgtggaa
     5101 aaattattat ttctaagatt gtggaaatgg caaaagcgat tgatattaaa gtaatctgtg
     5161 agggcgtaga aacttatgaa caagtagagt tcttgaaaga gataggttgt gataaggttc
     5221 aaggatactt atttgctaaa cctatggtat tagatgaatt tgaaaaacac ttaaacttta
     5281 aatttgatta atcattaata attattagac tataagatac cctaacaact tacttacaaa
     5341 tatgtaagtt aattgttagg gtatttttat ggttaaagta gtatattcac gatattatgg
     5401 tattgtagta aaaataaaaa attatggaga atataaaatg aacatattga gtattagaaa
     5461 tatatcaaaa acatattgtg gaaatattcc atttaaagca ttagataagg ttagtctaaa
     5521 tatagaaaag ggagaatttg tgtctgttat gggaccatct ggaagtggta aatctactct
     5581 tttaaatata atatctacag ttgatagaca gagtgaaggt gaggttgtat tagatggata
     5641 tgatgtaagt aaattgaaag gagaaaaact tgctgaattt agaaggaaac aacttggttt
     5701 tgtatttcag gattttaatt tgattgacac tttaactgtt ggcgaaaata ttatgttgcc
     5761 acttacacta gagggggcaa gtatcaaaga tatgaatata caaactaaaa gtatatctaa
     5821 atttttagga atagataaaa ttttagatag aaaaacctat gaaatttcag gaggtcaagc
     5881 tcaaaggtgt gctattgcta gagctattat aaataaacct gccatacttc ttgcagatga
     5941 gccaactgga aatcttgatt caaaatctac tgatgatgtt ttgaaattat ttactaggat
     6001 aaacaaagaa caaaaggtaa caacacttat ggtaactcat gaagcatata gtgcaagtta
     6061 ttcagataga gtaattttta taaaagatgg ttgtatatat acagaaataa aaaaatctga
     6121 aagcagtaat tctttctatt ctgatatact agctgttttg tctcaaatag gaggtgtaag
     6181 atagtggatt ttagaaagtt tgcttataat aatatagtta gaaatttcag ggcatattta
     6241 gcatattttt taagttctgt tttttctata atgatatttt ttgtttttgc agtgtctatg
     6301 ttccatccaa ttataacaaa atctggaatg cagagtggtt ctactgcatt tatggctctt
     6361 atgacttcag aattaataat attcggtttt agtctacttt ttatattgta ctctttagga
     6421 aatttcttaa aatatagatt gaaagatttt ggagtattga tgataattgg aatgagtaac
     6481 agacaactga aaaaattgat attaattgaa aatttgataa taggattttt agcagtgatg
     6541 ttaggaatat tacttggttt atcaatttca aaattgtttt tattgtattt aagcaaactg
     6601 ttttatatga atttaactga attttatttt ccagtaaaag caatgctatt gactattgta
     6661 tcatttatga ttttattttt gataacagga ccaatgagct taaaattatt gagtaaaaag
     6721 agcattttag agcttttagt aggtacaaaa aagcctaaaa aagagataaa atcttcaaag
     6781 ctatttggaa taattggtgt aatatgtctt atattaggat atgctatgat actatttggc
     6841 aataagttaa aaataaatgg aggtatgtat acaacagcac tattaattac tttggggata
     6901 tttttatttt tttctcaatt tactattttg atattaaagt atttaaaaaa taaagaaaaa
     6961 ttttataaga acaaagtaaa tatgttactt ataagtaatt tgatatataa attaaaggat
     7021 aatacacgat tgttattttt aataacaata cttttatcgg ctactttagt agcatttaca
     7081 acaacttcaa cacttgtaag ttctcaagga gaagcttcta aaaataattt cccaatggtt
     7141 tactcatatt tttcagagca taataatgaa aaagaatatg aagaactaca acaaattaga
     7201 gaagcaatca aaggatatga ttataaggag ttaagttttc ctgtacttaa atataatagg
     7261 gaagtcttac taagtgtaaa gaattataac aaattatcaa aggaattggg attagataaa
     7321 attagttcaa agttagaaaa atatgaaggg attataattc ctagtagcaa ttcgcaagaa
     7381 catataaatt ctttaaaaca attaaaaaat tatagtataa aaaatttaaa tatagaagta
     7441 aaaaaatcaa aaaataaaat aatttttcct acaggactgt ttagtaaagt tgtggttata
     7501 agtgatgact tatacaatga agtaaaaggt gaattttcaa aaattaattt tcatggattt
     7561 gattataaaa actggcaaag cagtgcaaag ataactgaaa atttaaaaga aaaacatttt
     7621 aatttggata gagaatataa caggtcatat tttttaacat taccagatat gtatttagta
     7681 gagttacaac aaagtaaaat tcttcaattt atgggtgttt ttataggagt gatattattt
     7741 atagcagttt ttagtttttt atacttcaga ttatatacag acgagataga agataatgta
     7801 aagtacaaaa aactatcaaa aataggtcta tcttttaaaa atatgaataa gattgttagt
     7861 atagagatag gtacattgtt ttttataccg tattttatag caattataaa tagcataatt
     7921 tctttactgt ttttaaatca agtattaaat aatagtttta agttagaaaa tttgggtata
     7981 ttactactga tatcatctat atattcactt tatttttact tattaaaaaa aaattatact
     8041 agaaaagttt ggttttttga ttaaagtggt atataataga tatataaatt aatatttaaa
     8101 aagtagttaa tattagggga gggcatttgc acctcccttg taatatgtgg aaaattgaat
     8161 attagaagaa ggaggagact tgatggaaaa tcaacaatta ttaggaacag aacgaattag
     8221 taaattacta ctaaaatatt ctataccagc tataataggt atgttggtaa atagtttata
     8281 taatgtagta gatagaattt ttattggaaa tataccaggg gttggaccac ttgcaataac
     8341 aggtcttggt gtaacaatgc caataatgac aataatactt gcatttggga tgttgatagg
     8401 tataggaact actactacca tatcaataaa acttgggcaa ggtaaagtag aagaggcaag
     8461 aaaacttata ggaaatgcaa tgacgttatc tgtaataaca ggaattatca taatgatact
     8521 tggaatatta tttgcaaata aaatacttac attgtttgga gcaagtgaaa atactttaat
     8581 atatgcaaaa tcatatatca atattatatt attaggtaca gtagtaaatc ttctttcctt
     8641 ttctttaaac cactcaataa gagctgatgg tagtcctaaa atttcagcag gaataatgat
     8701 agtaggatgt ttaactaata tagttttaga ctggatatta atatttggat tcaatttggg
     8761 aatacaagga gctgcaattg ctacagtaac ttctcaggct cttacagcta ttttaactat
     8821 agggtattat ataagtggaa aatcaaactt acgattcagt aaatctaatc taaaattaga
     8881 taaaaagcta ataaaagcag tgtttgcaat aggtatgtcg ccatttgcta tgcaacttgc
     8941 agcaagttta gttcaagtaa tatcaaatat tgcacttaaa actcatggtg gagatttagc
     9001 tataggggct atggctacta tttcttctat tgcaatggta tttttgatgc ctatctttgg
     9061 aataaatcaa ggagcgcaac ctattatagg tttcaattat ggagctgaaa aatatgatag
     9121 ggttaaaaaa gcatatttag gttcattagt agtagctact ataattttat gtatgggtat
     9181 ggtagtagtt atgcttttcc cagaagctat tataggtata tttaataaag accctgaact
     9241 tatgaatata tctgttaatg gattaagaat atacttactt atgttgccaa ttgtaggctt
     9301 atcagttaca ggaacaaact ttatacaatc tataggaaaa gcaaaaatgg ctatgttatt
     9361 gagcctttta agacaagtaa ttttattgat accagcagta ttaattcttc ctacattctt
     9421 aggtttacag ggtgtatgga cagctcaacc agtgtctgat tttatagcta cagtaattac
     9481 aggtatagtt gtatttagag aattaaaaag atatactcct aaaactgaaa aattaaatga
     9541 aaatgaaaga ctaaacgaga taactactga gtaaaaatat aaattcagat aattgagcaa
     9601 gaaattgcaa aaaatacgat tatatgataa aataaaaccg ctgtcaagga taagagaaat
     9661 tctaattatg gagtgatttt atggaattta caaagaaaag caaattaaaa aatatgtatg
     9721 taaacatgaa tgtagctaga atgttgtcta agataaatga gtataagggt agacaactgc
     9781 tttacaaaaa acaaccaaaa gaaattttag aaaacttaga aaaaaaatct ttagtagatt
     9841 gttctgaatc tacatacatt ggaaatcaaa gagatagtag taattttaat ctagaaaagt
     9901 tgataagtaa tgaagttaca cctagaagta gagaggaatt gagtattgtt gaatacagag
     9961 atgtggtaaa aactattaac agtgcttatg agagtattcc catcagttca caaacaattc
    10021 ttgagcttca tggatactta tataaatttt catctactag aggaggaagt tataagtcag
    10081 ataatgattt tattgaacac aatttaaaac ctagcgaatt tataagtact gattcaagtg
    10141 tcaataaagt tgaaaaagct gtagaagaaa tatgtgaagc ttataataca ttgatagagg
    10201 aagatgaaat tgatatatta atattaattt ctgcatttgt gttagatttc atattgatac
    10261 atccattcaa agaaggtaat ataaaaatgg cgagagtact tatcctattg ttactaaata
    10321 aaaatgggta tgaagttgga agatatataa gtttaggaaa gatatttgat gatagttcat
    10381 atgaatatta tagtaattta aattatttga aagcctcaat aggtagtgaa aaagcagata
    10441 tgaatgcatg gattgagtat tttttagaga cgatattaac tgcatatgag aagctggatg
    10501 atagtttaaa tatatctgat aagaaaagac aaacaaaaac tagtagaata gagaaaataa
    10561 taaattctac ccttggatat tttacaaaag aagatataag agatttatgt cctgacattc
    10621 cagaacctac tataaataga gtttttaata atctaagaaa acaagataag atagaagttg
    10681 ttgcaagagg aagaagtgca aaatggaaga aaaaatatta aaaaatgttt agataactaa
    10741 aatggtatag tgttttacac tataccattt ttaatgtcat tagcatgagc aataatttaa
    10801 caacttaagc aatatcaatt ttatttattg attatttttg aactgaaaaa tggcgttata
    10861 tggtatacct cagtggtaat taattttgga tgagaagata taatattaca gaatatatta
    10921 ggagtatatg tagaaaaaat ataataaaag tgtgaggctt agtaattaac aaattatgtg
    10981 gcttcacact tttttataaa ataaagatac ttagcagaat aatattttag ttgatatgtt
    11041 tataagatgg tacttgtttg ggtaaaagta taaatagtaa aaaagtagaa gtaatgtgta
    11101 taaaaacata agttacattc tgtaataata gttattttat aattcaacaa aagaatagaa
    11161 tgggtatatt tgtgaaataa taatataaaa taatcttagg agtgagctta tgaagaaaaa
    11221 attattatat ataaatgtaa attcaaaacc tgaagactta tcttcaagta aaactgtagc
    11281 aagaaaattt ataaataaat ttatggaaaa aaataaagat tttgaggttg aagagataga
    11341 cctttataaa gaacatatac caagacttga gtatcagtac tttgaaaaaa gaaatagcat
    11401 agttagtgaa gaaaatgcta aaaatttaga cgataaggat agaaaagagc ttacaaagat
    11461 tagaaatctt tgtgaccaat ttgtgaatgc aagtgtttat gtaatagcag cacctatgtg
    11521 gagtttatct tttccagcac cacttaaaga gtatatagat tgtataattc aagatggaaa
    11581 aactatatct tttgaaggca atgataaacc acaaggaatt ttaaatgata ttgatagaag
    11641 tatggtatat atacaatctt ctggtggaca cataccatgg gtgttgaagc cagttatgaa
    11701 taaagggtta aattatgtgg aaagtattat gaaatttata ggaattaaaa aatttgatga
    11761 attgttagta gatggaactg gaactagtga agaagaaaga caagctgcaa tagaaaaagc
    11821 atctgaaaaa atagatggta taatagatgg gatgaaattt taattataat attttattaa
    11881 aaatataaag gaaaaacctc ttttaatgta gaaacttata ttgaaggagg cgagaatcat
    11941 gcgagaagaa aaaagtaatg aaaagtatga ttgttattgg tgtaatcaag agaataattt
    12001 ctgtgtagaa ataaaagata atatagtcat gatagatgat ggcactggta cgttaaaaca
    12061 agccgttttc atagggtata aacaaatcca aattaattta aactgttcac attgtcaaaa
    12121 cttaaataga ataaaattaa atttgtagaa ttttaaaatg gaaattattg aaggaattaa
    12181 aaatagaaag tgttaatatt tagactgaaa attatatttt atacaataaa agtttattta
    12241 aataacattg tattttaata tttattttca gtcttacttt attaaaatta ttttttggtt
    12301 atgtctggaa aatatctgtc tactaatttt tctgaaatat tttttaattg gtcagccatt
    12361 gaagtcagac ttaataaggc tatcaataaa tcatctttat tttctggatt tatactaata
    12421 ttttcaaaat tagactctat aaagtccggt atattctttc tttgattttc ttttattaac
    12481 atgaattttt cgtaacatga aactaaatct ttttcactga aattatcctt ttcatacaca
    12541 ccatgtaaga ttcttttaat ttcttgtatt gtaagtgtat ttttcataga ataaataatt
    12601 atcatctgta aaatttgttc tttagtatac ttttttccct taactggttt gattaatccc
    12661 tctttgctgt aattatttat catggtttta gtaagaattt tatcactttc aaatctttta
    12721 ttagcagaaa gcttattgtc aattagagaa ataatttgat ccatatataa gtcaatagat
    12781 ggaatatcgt ttgaagttat atctgcatta tttagtgttt ctagtattat agtattaagt
    12841 tcattattca tcatatgtta cctccataat atatataata cactaaatta gaaagtaaaa
    12901 caatattaag gcttgtaaaa aaaacataaa gtggtataat taaatatata atatagtaat
    12961 caaaactata tttatgaatc tgatttttaa aatacataat tgtaggagga cgaaatgtat
    13021 caattttttt taaagggtag agacccaata agcagtttaa ctcattttat aggtgcttgt
    13081 ttatctcttt tagcaactat aattttagtt tttcaatctg ttacattaca ggaaacttct
    13141 ctcttgatga tagtatctgt atctgtattt ggactttcac taattgcttt atatagtgca
    13201 agttcgtatt atcatttttt aaaggggaca ccagaacaag aattgttttt tagaaaagta
    13261 gaccatgcaa tgatttacgt attgattgct ggctcttata caccaatatg tctgaatttt
    13321 atggaaaaaa aagaaggcat aatatttgtt acagcaatat ggatagttgc atttattggc
    13381 ataataataa aaatattttg gatggatgca cctagatggt tatctaccag tatatattta
    13441 cttatggggt gggcaattgt ttttgatata aatgcattca attcaattcc taaggattgc
    13501 cttagactat taattatgga aggtgtatct tactctatag gtgcgattat ttatataatt
    13561 aaaaagccta atataagtcc tgagtttgga tttcatgaaa tatttcatat ttttattatg
    13621 ataggttctt tatttcactt tttagcagtt ttattgtatg ttttatagtt ttaaaagatt
    13681 tgattaattg atactaaaat tctacaatga ataaaatgga actgccttat atagattata
    13741 tcttagacaa taagacagtt ccttattata aatattaaat tctacttata ataaaatttt
    13801 gataaaaata gtccattcaa aatgcctatt ccataattcc tttaatgtac ttttcattta
    13861 cgacatatag tttgaattta tgtactataa ttatatcctc taaattctct gaaacactaa
    13921 caattctgac acatttgttt ctaatttcta caggtaaatc taaatcattt gttaaattca
    13981 catagtcatc atatttatac ataaaatcat ccctttac
//
LOCUS       DAMJWS010000099        13339 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMJWS010000099 DAMJWS010000000
VERSION     DAMJWS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.103"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            133..1992
                     /locus_tag="PC399_003193"
     CDS             133..1992
                     /locus_tag="PC399_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2594029.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2284..2883)
                     /locus_tag="PC399_003194"
     CDS             complement(2284..2883)
                     /locus_tag="PC399_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2594030.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGNDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2986..3783)
                     /locus_tag="PC399_003195"
     CDS             complement(2986..3783)
                     /locus_tag="PC399_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2594031.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3896..4001)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4093..5079)
                     /locus_tag="PC399_003196"
     CDS             complement(4093..5079)
                     /locus_tag="PC399_003196"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2594032.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5563..7293
                     /locus_tag="PC399_003197"
     CDS             5563..7293
                     /locus_tag="PC399_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF2594033.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7403..7483
                     /locus_tag="PC399_003198"
     tRNA            7403..7483
                     /locus_tag="PC399_003198"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7437..7439,aa:Leu,seq:tag)
     gene            7818..8333
                     /locus_tag="PC399_003199"
     CDS             7818..8333
                     /locus_tag="PC399_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HDF2594034.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8825..8929
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9139..11613
                     /gene="gcvPA"
                     /locus_tag="PC399_003200"
     CDS             9139..11613
                     /gene="gcvPA"
                     /locus_tag="PC399_003200"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF2594035.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11613..13070
                     /gene="gcvPB"
                     /locus_tag="PC399_003201"
     CDS             11613..13070
                     /gene="gcvPB"
                     /locus_tag="PC399_003201"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF2594036.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         5002 a         1581 c         2195 g         4561 t
ORIGIN      
        1 aactatgtaa aacaaggttt atatattcta attgtataga aaatttattg taaacgcatg
       61 tatattatta gaaaaaatag tttgaaggta ttcaataaac ataaaaaaca atatagggga
      121 taaaggtgtt ttatgaagag atttttgagg agaattatct tagttttatt tattttgttg
      181 atttttataa gttttatttc tattaaatta attcataatg ttggagatta tggaaaattg
      241 attaattatg ttgggattgt tcgtggagct agccaacgtc taactaaact agagatgaat
      301 catcaaccaa atgatgaatt aatagaatat attgatgaaa tcctacagga gttgattaca
      361 gggcatggag attatggttt agtaatcact gattgtaatg aatacaatga ggacttactt
      421 ttattagaaa aaaagtggga agatttaaat attgaaatta aaaaagtaag aatgaaagaa
      481 cagaataacc agttattaag tataagtgaa gagttttttt ctcttgcaaa tgatacagtg
      541 tttaaaatag aaaacttttc taaagaaaaa agtaattact taatgacttt aataataatt
      601 atatctatta taggtatatt agcatgtatc atattgattt tacaatatag taaaaaaatg
      661 gttagactgg aaaaattaaa tgtagattta aaaaatattg catataaaga tgaactaaca
      721 ggagtaaata ctatagaaaa atttaaattg gatgcaaatc aaaatatttg tatacataag
      781 gataagaaat ttgctgtatt ctatattgat tttgaaaact ttaaatacat taatgatata
      841 tttggatatg attatggtga catgatactg aaaagatatg ctaatcttat gatgaatgac
      901 atagggaaat atgaaatatt tgcaagagaa attgcagata gatttgttgc gttaagatgc
      961 tatgtagata aggaagattt agtggtaaga caacgagatg tagacaatga actaattaat
     1021 ataacaaatg aaattaaaaa taaacatagt attactgttg ttagtggaat ttgttgtatt
     1081 gaagatgtaa atgaaaaatt aagtatagat ggtttaataa atagagcaaa ttttgcacaa
     1141 aaaactgtta agaataaacc aggtactaat tatgcttttt ataatgacag tattcgtaaa
     1201 aaaatgattg aagaaaatac tatcaagagt agaatacatg aagctattga aaaaagagaa
     1261 tttatagttt atttacagcc taaagttaat ttacatagtc aaaaaataaa ttgtgcagaa
     1321 gctttagttc gttggcttac tccggataaa ggtattatat cgccagcaat atttattcca
     1381 gtacttgaga agaatttttt tattgcctta gtagataagt atgtttttga ggaggtttgt
     1441 aaatggatta gaaagaggtt agatgaaaat aaaccttttg ttcagatatc agttaatgtt
     1501 tcaagaatac aattttataa cactaaattt gtagaaactt attccaatat acaaaataaa
     1561 tacaggatac ctaaaaatac tatagaaatt gaatttacag agagtgttgc ttttgagaat
     1621 caaaaacatc ttttggaaat tatacatgat ttgcatgaaa atggatttac ttgttcttta
     1681 gatgattttg gaaaaggtta ttcgtcttta agtgttttaa aagatttacc atttgatgtt
     1741 ttaaagcttg attctatgtt tttcaaggct agtttagata aagataaaga gaaaatagta
     1801 ataaaaaata ttgtacatat gttaaaagaa cttaatatca caactgttgc tgagggcatt
     1861 gaatgcaagg aacaagttga atttcttagg gatattgggt gtgatttagt tcagggattt
     1921 gtattttata aaccaatgcc aatattagaa tttgaaaaaa tattggataa agagtttgtt
     1981 tataatagct aaaatgtact ttataaaata tgaactaatt aagtaaaatt attttgtact
     2041 gaataaattt ttactttatt tacagaataa tgattattta cagaaaagcg cgaaaagtgt
     2101 attcctttaa gtgtgggatg gatagcacaa atagaggaga aaaaatattg gccatataag
     2161 aatgcataat aatataaata tttgaaaggt cagcattata taaaaattaa aaagaaaata
     2221 aataattttt ctataaaaaa gagactatga gaaaatttac tcatagtctc ctaaataatg
     2281 tatttatttt cttcctgcaa cccattttaa tccccaatga aattcagtat ccatttctct
     2341 atgacctttg aagtatttaa ctactttttc aacactgaat gtctcatcat ttacattttc
     2401 tatcaatgct attgaacaca tattatagcc atttttatgt tcatcaagtt tgacaactaa
     2461 gtctgaatct tgtttttgtt tgattgttac aattccatca gcttcagacc agttagctac
     2521 accttcgtat ataaaagcat aaataagtat tctttttata tcttttattt tattaccatt
     2581 tattcttaaa ttttctcctt gtgtattact tcctgttctg tcatttccat ctaattgagc
     2641 aaaaggtgga ttgtctaaac taccaaaggc atttcctaat gcttgaacag ctccttttac
     2701 accatttttc atctcaaata ggcaacccaa atctaaatct atattactgc ttcttaaaga
     2761 gccaaaaaaa cccttttttt gtactttttg attccaattt aaattaacta agatttcacc
     2821 taaatcgcta ttttcctttt ttgtaaggtt tattttttgt ccctttttta attctatagc
     2881 catagtaatc ttcctccaca aacatagatt tatttaaatg gtagcactta ataagtcaat
     2941 attcaacaag attagaataa taatattaaa attatactaa atatactaaa aagtaggaat
     3001 cacaatacct ttgtattttt cttcaattaa gcttcttgct ttatctgatg taagaacatc
     3061 ttgtagaact ttaatctttt cactgtcttt atcttcagtt ctaaatgcta aaagatttgc
     3121 atactcgtca tttataggag ctgtataaat tccgtttttc ttggcatcaa gaccagcagg
     3181 tacagcataa gtagtatcta taaatgcagc agtcacatct ggaagagaag atggaatttg
     3241 agcaatatct aactctttga attgtattcc ctttggattt tctattatgt ccttaggact
     3301 aactaatcca tcccctttag gtaacgttaa aagtccaata ctttcaaggt atctaaggtt
     3361 cttagaacaa gaagatgggt tattgcttat tgcaattgta tctccttttt taaattcatc
     3421 aacagatttt atcttataag agtaaagtat tgctgggcat acatatagtt ttttaccagc
     3481 cattattttg taacctttgg ccttaacagc ttcttttaga taaggttcat gttgaaatag
     3541 atttgcatca atttcaccat tatttaaagc ttcgtttgga agtatgtaat catcaaagtt
     3601 tttaacttct aaagtatatc ctttttcttt aataagtggt tttaattctt ctaataactc
     3661 tcctcctgga actaaagtag caccaacaac aattttttta tcatctttag ctttagaaca
     3721 tccaaccaca gctaaagtta atactagaca taataacaaa cataataatt ttttgaattt
     3781 catataaatt ccctcctgaa tataattgat tagttaaaaa taaaatacca ttctttatca
     3841 cactgcaaaa taaaaataag cctgagttta aactcaggct ttaaaagcta atttagactc
     3901 atctttcagt ataatactgc aggatttagc accaatgtat ataaaaatat actggttgct
     3961 gggtttcata gggccagtcc cttcaccact ctggataaga agtatcaatt tttaattatg
     4021 ttataaatat tatcatgatt ttttacgtct gtaaacataa ttttataaat attttaaaat
     4081 ctttaatgta ttttatttaa cacctaaaat tccagaaact agaatatcag tatcagcacc
     4141 taagaaatag tcatttattt caatagaatc cagagtattt ctcaaagcat tttcttcatg
     4201 ccttatacca ataagtttat tttctacaaa tgcaacatca cattttccaa aaaaatctcc
     4261 aaaaaatttt atactagtta ttatgccttt atcaacattt aaattaaact caacatttcc
     4321 accagaatat tttaattcat ttgaaagagt atattttggt gaattaccaa aattccaatc
     4381 ccaagtttcg tattttgatt tagtaagctt ttctatttgt tttatatcat gctcactcaa
     4441 acaataatta gtattatcag tatttgtttt agaaagataa tccattatag catctttaaa
     4501 ttctaatatg tccatttttg aatgtaaatg ttcacttata ttggttacac gagattttac
     4561 agattttatt ccttttcctt caaattttat aggtttaacc tttaatgctt ttgagaggtc
     4621 atttatttca gatgaaaata aaagagtacc atgatgcatt actttgtttt tgtagttgta
     4681 ttgagcattt ccagaaaatt tcttgccatc tataagaagg tcatttcttc cagaaaattc
     4741 ggcattgaca tctagtgttt taagtaaatc tattatgggc tgagtgaatc ttctgaaatc
     4801 actaaaatta ttattgttac aagctataaa tgtaaaattt atatttccca aatcatgaaa
     4861 tacagctcca ccaccagact gtctcctaac gacaggtata gaatattctt ttatatattc
     4921 taaatttatt tcagaaagtg tgttttggtt ttttccaaca attatagaag gttcattcct
     4981 ccataaaata aataagtctt catcaaaatt ttttagaaga tattcttcca ttgctaaatt
     5041 aaaataaggg tttgtttttt cattataaat taataacatt aaagtagttc ctccatagaa
     5101 ttgataaaat ttttattatg aatacaatta attttataat tcgacaataa tataaaaact
     5161 cctttaaatt agaaatttta aaaactacct tgcaaagttt aaataaataa tatatactca
     5221 aaatatgttt aataaaagtg aatagaaggg tagaggcgca ataatctatc agtactaata
     5281 aggagttaag cacagagatt tattaggaaa ggagatattg ccgaagtttt ataatcgatg
     5341 ctttaagatt atattgctgg gggtacatag aaaatatgta caactgtcac aggtttattt
     5401 gtggtgagct atcatttaag gaattatatt atatttattt ttactattgt tgatatattg
     5461 taatgtttaa gccttgagtg tttaccactc aaggcttttt tagttttatt gtaacataat
     5521 accctgtaca gttcacaaaa ttaaaaggag agggagattt atatgaggaa caaaaaaatt
     5581 aatattattt ttctgacgac aatcatgttc ataatgtcaa cagtaatggt ttttgcagaa
     5641 gaagatatag acactattgc actagccaat gcagaaaaat ttgggatttt aactttaata
     5701 ccaccattag ttgctataat acttgcattt ataactaaaa acgtaataat ttctttactt
     5761 ataggtattc tttctggtag ttttataatt aaagctagtg gtattaatgt ttttgcgaca
     5821 tttatacagg catttttaga tttagtagac agagctttag tatctttagc agacccttgg
     5881 aatgcaggga taatactaca ggttttggca attggaggag taataaatct tgttgctaaa
     5941 atgggaggag ctaaagctat agctgaagca cttgctaaaa gagcaaagtc agcaaaaggg
     6001 acacagctta taacatggtt tttggggctt ttagtattct ttgatgatta tgccaattca
     6061 ctaatagtag gtcctatgat gagacctgtt gcagataaaa tgaaaatatc gagagaaaag
     6121 ctagcattta taatagatgc cacagcagca ccagttgctg gtttagctat aatatctaca
     6181 tggataggtc ttgaggttgg tttaatacat gatgcttttg aaagtataag tatagatgta
     6241 gatgcatttg gtatattttt aaatacaata ccatttagat tttacaatat attaatatta
     6301 gctttcattg taatatcagc attattatta aaagaatttg gtccaatgag aaaagctgag
     6361 ataaaatcta gaagtagaaa aattagtata gaccttgatg aaggcgtaga agagttggat
     6421 gatttagctc caaaaaatgg agtaaaattg agtgtatgga atgctataat cccaatagga
     6481 acattgataa tagttgcatt agcatcattt tattatagtg gatatacatc tataatggga
     6541 ggagacgata aggctctgat acaactattt acaaattctc catattcttt tgaagcaata
     6601 aaagaagcat ttagtgcatc tgatgcatct agagcattat tccagtctgc acttgttgca
     6661 agtttagttg ctattataat ggctgtagta aaaaagattt ttacaatatc agaagcaatt
     6721 gatgtttgga tagatggaat gaaaagttta gtaatcactg gtgtaatatt aatacttgca
     6781 tggtcattaa gttcagtaat aaaagagtta ggtacagcta aatttttaat acacctatta
     6841 tcaggttcac taccaccatt cttattacca agtttaatat ttggtttagg agcaataata
     6901 tcttttgcta ctggaacagc ttatggaaca atgggtatac ttatgccact tgcaattcca
     6961 ttagcatact cattaaatcc tgatatgtct tatgtaatag taagtactag tgcagtttta
     7021 acaggtgcaa tatttggaga ccattgttct ccaatttcag atacaacaat actttcttca
     7081 atgggagctg gctgtaatca tatagaccat gttaacactc aaatgccata tgctatattt
     7141 acagcagtaa tcactattgt atttgggtat ataccagctg gacttggatt gccaatatat
     7201 atagtattac cagtggcaat agctgctata tttgtaggta ttcaaataat tggtaagaaa
     7261 gttgatgaag cggaaataga acttgtagaa taaagatata aataagtttc accttttatt
     7321 atcactattt tgttagtttt tagataattt taaaaagtta ttgaaaataa tggcgaaata
     7381 tagtaaaata aaagagtgat aagcggatgt ggcggaattg gcagacgcac tagacttagg
     7441 atctagcgcc attggcgtgg gggttcgact cccttcatcc gcactttaca gtacttaatt
     7501 gtactgaaaa aaactggggt tgctggcttt cggagtgggc tatcccgttt ttattttttt
     7561 gtatatataa tagattggca ctttagtata aggtgctttt tttataggca aaaacagtac
     7621 atttatttct gttttattat cattcatcta ttagaatata ctttagttaa gaatgtgtta
     7681 ataagcagtg ttcacattct tgataaaatg tggaataata tgtattttta ctaagacgga
     7741 aatgaaataa ttaaaccatt aaataatatt ataagatgat gaataagtag ataaatcaaa
     7801 ttttgtagga ggatattatg gaaaaatata tatggttgtt tccattactt tttatttttc
     7861 atgatatgga agagataata ggttttggaa tatggcttaa aaaaaataaa tcaatgcttg
     7921 ataagaagta tccatttatc agtaaagttt atgaaaatta tagtactgaa ggcatggcat
     7981 ttgctgtgtt tgaagaattt attttatgca ttattttctg tatacttaca gttataacag
     8041 agaatcagta tgtatatcta ctatggttag gtagttttat agcatatacc ttacatttgg
     8101 taattcatat agggcagtca atcataataa gaaaatatat accttcactt attaccagta
     8161 tcatatgctt accaataagc atttggtgta taagtaagag tatttatata gttgattgtg
     8221 aaatgagtac aacaattctg tacagtataa ttggcattat aattgtagca ttgaatttaa
     8281 agtttgcaca gtcgctgatt ggaaaattta caaagtggat gtcaaacata taaattttag
     8341 tttattggac agttcaagga atcagaaatt ggaattttat agtattaata ttttatggta
     8401 ttaatataat gtataaaatt ttatagcaat gtatttcata agtataaagt gactagaatt
     8461 ttaagaaata tacaaattct ataaaaacta aataatttca agtatatttt aaaaatctta
     8521 tttttcacaa gtttaaaatt gtaaacgatt tcatcaagtc tatgaatata cttttaacat
     8581 gttatagtaa atctcaatat aaatttacat ttggttcttt gtgtgttatt ttaataataa
     8641 attaagtgaa aatgttattt tacattaata tataatatct ttgcataaga gtgaataata
     8701 taaaagagaa ataattgata ataaaaactt aacaaaaaaa ttaatacata aaaaaaaata
     8761 aatataatgt gttgacattt aattgaaata ttcatacaat aaaattgaaa ataaatattt
     8821 ccaaatgaga ttagcgagag agtcctttat acgaaaaggc gccgaagaag aaatttaaga
     8881 aatgccaatt tttagagaag ctttcaggca aaaatatcgc taattgatgg gactctggag
     8941 agacttaagt taagatacta taagtcaccg aagaagcaaa ataatattaa attattaaat
     9001 ctttcaggta tacaaacagg gatatatagg ctaggattat tttccatgct gatatatctt
     9061 ttttttatga aaaagtacag gaaataatat caaagttact taaatacagt aggtctaatt
     9121 ttctagggag ggttatatat gaatgaattg aaaagagtat ctttgtacaa tatacacaaa
     9181 gagcttggag caaaacttgt tgaatttgct ggttgggaaa tgcctttaga atatgagggt
     9241 ataaataaag agcatgagaa ggtcagaaaa agtgcaggta tatttgatgt atctcatatg
     9301 ggagaggtac agataaaggg agcagagtca gaaaaattta tacaaaacct agttacaaat
     9361 gatataagcg cattaaagat aaatgatatt atatatacac ctatgtgtta tgaaaatggt
     9421 ggagtagttg atgatttatt aatatataaa tttggtgaag aagattattt attagtaata
     9481 aatgcaggta atatagataa agatgtagca tggataataa aacaaagtga aggatacaat
     9541 gtagatataa aaaatatatc tagtgaagtg agtcaattag caattcaagg acctaaagca
     9601 gaagaaattc ttcaaaaaat cacagatata gacttaaact caataaaatt ttataagtca
     9661 attccatcaa caaaagtatg tggttgtcct tgtttagttt caagaacagg atatactggg
     9721 gaagatgggt ttgagatata ctgtaaaaat aaatatgtag aaataatttg gaatgaagtt
     9781 ctaaaggttg gaggagaaga tatatgtcca gctggtctag gttgtaggga tactttgaga
     9841 tttgaagcag ctctacctct atatggacat gaaataaatg aacacatatc tccaattgaa
     9901 ggtggtttat ctatctttgt gaaaacaaat aaagaatcat ttattggcaa atcaatttta
     9961 tcaaaagaaa aagaaagtgg agctaaaaga aagttagtag gatttgaaat gcaaggtaaa
    10021 ggaatgccta gaaatggata tgatataagg attggtgata aaacagttgg atttgtaaca
    10081 acaggatgtg catctccaac gacaggaaaa attttaggta tgggaattat agattctgaa
    10141 tatgcaaaag taggaaatga aataggtata gcaatcagaa aaaaagttgt tcctgctgtg
    10201 atagttaaga aaccttttta taaaaaacaa tataaaaaag ataatattat attaaacaaa
    10261 gaaaataaat tttcatatat acctgctaca agtgaagata aatcaaaaat gttaaaagta
    10321 gttggattaa attcggttga tgaattattt tcagatatac cagaagaagt aaaattaaaa
    10381 agagatttaa atttggaaat tggaaaatct gaattagagg taagcaaaat agtaaaaaga
    10441 ttgtcagaag aaaatttaag tttagaagac ttgacttgtt ttcttggtgc tggtgcatat
    10501 gaccattata taccatcaat tataaaacat ataacttcaa gatctgaatt ttatacagca
    10561 tatactccat atcaagctga aataagtcag ggaactttac aagtagtttt tgaatttcaa
    10621 tctatgattg cagaaattac aggtatggaa attgcaaatg catcaatgta tgatggagca
    10681 actgctgcaa tagaagcatg tataatggca atgaatcaga ctagaaaaag taaaatagta
    10741 gtgtcaaaaa ctattcatcc tgaaactttg tcaatattaa gaacttactt acaatataaa
    10801 gattgtgaga tagtagaaat tgacttttgt aatgaatatg gcactacaga tatagaaaaa
    10861 ttaaaagctt ctgtagacaa agatacagca tgtgttctta tacaaactcc taatttcttt
    10921 ggaattattg aagaaatgga agaaatagaa aagataactc atgaaaataa ggctatgtta
    10981 attatgagtg tagacccaat atcattagga gttttaaaaa caccaggtga gataggtgca
    11041 gatattgttg ttggagaagc tcaatcactt ggaaaccctc ttaattttgg aggtccttat
    11101 gtaggatttt tagctagcaa atctaaatat actagaaaaa tgccaggaag aatagttggg
    11161 caaagtttag atgtagaagg taaaattgca tatgtattaa ctttacaaac tagagaacag
    11221 catgtaagaa gagaaaaagc aacttctaat atatgttcaa atcaagcttt aaatgctctt
    11281 gtagcttcaa tatatatagc tactatgggt aaagaaggat ttaaagaggt tggtatgcag
    11341 tctatgaaaa aagcacatta tacttacaat aagcttgtac aaactggaaa atataagcca
    11401 atatttaaag gtaagttttt caaagaattt gctgtacaag gaaatcttaa tatagaaact
    11461 ataaatgata aactcttaga agaaaatata ttaggtggtt ataatttaga atacaattat
    11521 ccagaattaa aaaattcaac tttgctttgt gtgactgaga aaagaagcaa agaggaaata
    11581 gataagttag ttggaataat ggagggatta taatgaaaga atacaatagt ttgttgatag
    11641 atatatctaa aaaaggtagg aaagcatact cacttccaaa gttagatata gatgatataa
    11701 aaatagaaaa tatgattgac caagatatgg caagacaaag tgagttgaat ttgccagaag
    11761 ttggtgaatt agaattagtt agacattata cattattatc aaataaaaac tttggagttg
    11821 atacaggttt ttatcctttg ggttcttgta ctatgaaata taatcctaaa ataaatgaag
    11881 atatggcagc acttccaaac tttactggaa tgcatccata ccaatcttcg gatacagctc
    11941 aaggttctct ttctttgatg tatgatttat caagaagact tgcagaaatt acaggtatgg
    12001 atgaagttac actacaacct tcagcaggtt ctcatggtga gtttacaggg cttatgatta
    12061 taaaagcata tcatgaaaat agaggtgacc ataagagaac caaagtgata attccagatt
    12121 ctgcacatgg aacaaatcca gcaagtgcag ctatggcaaa ctttgatgtg attcaaatag
    12181 catctgataa aaatggagct gtagacataa atgcattaaa agaggttttg aatgatgaag
    12241 tagcagctct tatgcttact aatccaagta ctcttggttt atttgaaaaa aatataaaag
    12301 aaatagctac tcttgtacat gaagcaggag gacttttgta ctatgatggt gcaaacatga
    12361 acgctattat ggggataaca agacctggag atatggggtt tgatgtggtt catcttaatc
    12421 ttcataagac tttttcaact cctcatggag gtggaggtcc aggagctggt cctgttggag
    12481 ttaaaaaaga attagtacct tttttaccaa taccagtaat tgaaagaaaa gaagataagt
    12541 atgtactaaa ttatgataga gaaaaatcta ttgggaaaat taaaaatttc tatggtaatt
    12601 ttggagttct tgtaagagct tacacatata tactaactat gggaagggat ggacttaaag
    12661 aggctagtga aatggcagtt ttaaatgcta attatataaa ggaaagtata aaagatgatt
    12721 atattttgcc aatagatacc ttgtgtaagc atgagtttgt attaggtgga ttgccaagag
    12781 gagaggccaa tataaaaact atcgatattg caaaaagact actagattat ggataccatc
    12841 ctccaacaat gtactttcct cttattataa atgaggcact tatgatagaa cctacagaaa
    12901 gtgagagtat agagacatta gatagtttta tagaagctat gaaaagtgtt gctaaagagg
    12961 caaaggaaga accagaacta ttgaaaactg ctccacataa tactttggtc aaaagagtag
    13021 atgatgcaag ggcagtaaag aaacctatac taacatggtc acaaagataa atagttaaaa
    13081 agtagagatt taaagtataa agagaattaa atttgtaaga aaaagctatc taacttaatc
    13141 tagtttagat agcttttttg tatattagta tgtatggtat tttctatata aagttttaaa
    13201 ttatattagt gcttatatag aatattgata tgatgattat gtatagatat taataagaat
    13261 tgttgaattt atcttgcctt aactttattt actcttttat attaagaaaa acttaactaa
    13321 aatatagcga aattcataa
//
LOCUS       DAMJWS010000100        12820 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMJWS010000100 DAMJWS010000000
VERSION     DAMJWS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.104"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            93..476
                     /locus_tag="PC399_003202"
     CDS             93..476
                     /locus_tag="PC399_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594037.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            984..1367
                     /locus_tag="PC399_003203"
     CDS             984..1367
                     /locus_tag="PC399_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2594038.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1369..1719
                     /locus_tag="PC399_003204"
     CDS             1369..1719
                     /locus_tag="PC399_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594039.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFVDKK"
     gene            complement(1820..2278)
                     /locus_tag="PC399_003205"
     CDS             complement(1820..2278)
                     /locus_tag="PC399_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594040.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2323..3111)
                     /locus_tag="PC399_003206"
     CDS             complement(2323..3111)
                     /locus_tag="PC399_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2594041.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3250..3687)
                     /locus_tag="PC399_003207"
     CDS             complement(3250..3687)
                     /locus_tag="PC399_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HDF2594042.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4509..5018
                     /locus_tag="PC399_003208"
     CDS             4509..5018
                     /locus_tag="PC399_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2594043.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            5432..5863
                     /locus_tag="PC399_003209"
                     /pseudo
     CDS             5432..5863
                     /locus_tag="PC399_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(6050..6243)
                     /locus_tag="PC399_003210"
                     /pseudo
     CDS             complement(6050..6243)
                     /locus_tag="PC399_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            6345..6851
                     /locus_tag="PC399_003211"
     CDS             6345..6851
                     /locus_tag="PC399_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2594044.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSALDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            6856..7132
                     /locus_tag="PC399_003212"
                     /pseudo
     CDS             6856..7132
                     /locus_tag="PC399_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
     gene            7401..7973
                     /locus_tag="PC399_003213"
                     /pseudo
     CDS             7401..7973
                     /locus_tag="PC399_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            8076..8891
                     /locus_tag="PC399_003214"
     CDS             8076..8891
                     /locus_tag="PC399_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2594045.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            8972..9448
                     /locus_tag="PC399_003215"
     CDS             8972..9448
                     /locus_tag="PC399_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2594046.1"
                     /translation="MTTYKVNSIGTICNNEKGAFIQLEAPYILALRALDGFSHINVLW
                     WFSDFDNTNFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSAVEIIDIDYMSGLI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPQVPDWCSHWPHSLEESASFAWESEFNF"
     gene            9462..10025
                     /locus_tag="PC399_003216"
     CDS             9462..10025
                     /locus_tag="PC399_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2594047.1"
                     /translation="MSLQDYIFEKPVIETEHLILRTMNKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPELLFDKSKKSTKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKHYQGNGLMSEVLKNVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITRWCYG"
     gene            10280..11191
                     /locus_tag="PC399_003217"
     CDS             10280..11191
                     /locus_tag="PC399_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2594048.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKR
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     SEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEIWIIPV"
     gene            11269..11703
                     /locus_tag="PC399_003218"
     CDS             11269..11703
                     /locus_tag="PC399_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2594049.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            12171..12820
                     /locus_tag="PC399_003219"
                     /pseudo
     CDS             12171..12820
                     /locus_tag="PC399_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         4621 a         1820 c         2176 g         4203 t
ORIGIN      
        1 ttggaataaa ctatacttta aatatagata tttaatttaa ataaatttta cagtattaat
       61 agtaaattat cttagaaagg aatgtgcttt atatgtctac agacaaagtt gttggaatca
      121 tagatgaaga aacggcagaa cttgcaggaa ttgaatatac tggtaaaatt tatgcttctt
      181 ctggtgttat aaaacatata aagaaaaaac atagatgtca actgagtaag gatattttta
      241 atgatataat agacactata aaaatggtat taaagtcacc tgaatatata ggaagtcatc
      301 ctaaaaaacc tggaaaaagt gtggagttta taaaaaaaat tgatgattat attttagtag
      361 ctacagaatt agatacaaaa aaaggatatt tatatgtatc ttcaatgtat gctataaaag
      421 aagcaaaatt agaaagtaga gtaaacagtg gaagagtaaa acaatatgat ggttaacata
      481 atattattaa ttttccctaa aatattgaaa aatattatta tataagttat aatattaata
      541 tacacgtaga tatttattag ttagatttga ggttggaaaa ggcacccaat acgctactat
      601 agagtagtta atagagatgc aggatacgcc gccctgccaa atctaattaa attaaaaaga
      661 gaggattaat tcctctctta ttgttttttt gagatactaa tttaatctat aatagaaaga
      721 ttgaaggtcg tttatacaaa aaaattttca gttatataca ttaaactaat taaataagat
      781 atttataatc tatatgtgat acatattata tattaataaa agaaaaatat atagaaaatc
      841 cttataaaaa tttaaattgt aatgaatagg aagtaattaa aaagaatttt tattgatttt
      901 taaaaattta gttagaaact atatcattat ggtaaaaaat gtattataat gtttagaaat
      961 attataataa ggagcgatta aacatgaatc tatcgaagaa aatagaagat aaaagaaaag
     1021 ctaaacagag agctgaaaaa gttaagaaag ctaaaattgc tacagctggt gtggtattag
     1081 gagctgttac tggtgcagtg agtggagtac ttttggctcc taaatctgga aaagaaacaa
     1141 gagaggatat aaaagatgct tcacaacaaa ttgctgaaaa gataaatatg aaaactgtag
     1201 atgtaaaagg caaagtatct gagaagctag aggataaaaa aggaaacttt atagaatcaa
     1261 agaaaaaaat aaaaaaatat atagataaca aaaagagtgt tgaaaattct agtgaggaag
     1321 ataaaataat agatatagtt gaaccagtta gtgttactga ggaataatat ggacaataca
     1381 ataacgatta acataagtat aatatatgta ttattagcta tacttgcttg tattgcttta
     1441 gtttatttaa ttattgtact tgttaaacta cataagattt taaataatgt tggaactatg
     1501 ttaaatgaaa atactcaaaa tataaataca actttaaata atcttcctaa tgttgtagaa
     1561 acttttggag aagtaggaga aaatatgaaa gatgtaacag aagtagttac tgaagttaca
     1621 gcagattttc ttgttactaa agacagtgtc aaatcaaata tggatgttat agttgatata
     1681 ttgactataa ttaaaaacat ttttgtagat aaaaaataat aagttaatat aaaataagcc
     1741 tataagatat aatttaaacg accttaaaat agcatttata aggtcgtttt ttattagctt
     1801 gatttgacac tatgtgtttt taataattgg taatattcct ctttatcatt tgaacatata
     1861 tatctatgct tgttgtttca attacatcca taagaacatc tttacattca agctcaaaat
     1921 cttccattcc ttctaaatat tcatcatctt catcaaaaaa gatataagaa ttatatagcc
     1981 attcttcaaa tttttcttca agtatatccc aatacgaact ccaaaataat ccacaacctg
     2041 atatataaga tgctctactt tgatttatat aaacttcatt tagaagcttt tgtaatctac
     2101 tgctataaag aaaatctgct cttccactgc ttgatattaa ttcatttaaa tcttctattg
     2161 acattaattc aactttacca cttttatcat aaggagtata attttcatca acccatttat
     2221 gtggtttgtt ttctctgcat actgttgcta tcaactctac aacttttgtt cttatcattt
     2281 ttttatccta acttatttat tttttataaa ataatcatga ttttataatt tatctatttc
     2341 ttgtaatgct aatttaacag tttcatatct atcagcacca tttattacta catcaccttt
     2401 tacaagttta ttagcttctc cacctacaac ataaaccttt ttacctaaac cttgcttata
     2461 actagacgct tcaattatta aagaatcttt caattgccaa tgtaaaatct gagcagccac
     2521 tttatcagca tcacctgtat aaacgattac attttcatac actttatttt cccctccccc
     2581 ttgagaattt gatatagatt tgtttagtat accttctgct atcaatttag caactttatc
     2641 tttattatta acataataat ctgtatctgc tttactatct acaaaacata cttctattaa
     2701 tattgctggt gcaatagtat gattaagcca ataaagacct ctaacatcag actttgcacc
     2761 cctatctttg aatactgtag ctaacctagt attcactctt tctgcatagg ttttgccatt
     2821 attagtctta tatattgttt ctgtccctac tggagttgaa gttgtagcat ttgaattaaa
     2881 atgaatctga acagccaaat cagtttcctg tttatttgct atagcacatt ggtctgctaa
     2941 atgattactt gattcatcaa ctcttcctgt ataaacagta gctccaccta ttttaagcca
     3001 tttaacaatt aagtcattca gtattcttgt ttctttgctt tcgtttatat acccaacagc
     3061 tcctgtacct tttccaatta aagtgtgccc tggtattatt actactttca aactaacatc
     3121 tcctttacaa ttaaatttaa cttctattta atatatgcat aacctagaat tttgtttctt
     3181 tttatatctt atacttttat tataacccat tttgtaaaaa tatagaatat taagtaaata
     3241 atgttaaaat tataattttg tatataagac attacttgat tgcaataatt ttatagcata
     3301 ttcatctatt ctatatccag ttctataata tatatgttta attccagctt gaattatagc
     3361 cttagtacaa tttaagcatg gaaagtgagt tacataaata gagcaatttt taactgaaat
     3421 accttctttt gcacaataat aaagggcatt ttgttcagcg tgaatggttc taatacaatg
     3481 tccatctctc atttcatgac caacttcact acaatgctta tctccagaaa tactaccatt
     3541 atatccagta gacactattc tgttttctga attgacaatt atagctccta cataagctct
     3601 atcacaagtt cctctttcag ctactgtttc gcacagtctc ataaaatatt cttgccatga
     3661 gcatctattt tcaagttttt tattcaaatc atatacctcc aaaatttata tctaatatat
     3721 aagatttata ccttaaaaat aatattaaaa acaaatttat atgataaata aaatttatta
     3781 atactgtttt tagtctaaat tatttcattt taaattaaag ttacaaggaa tctaagatta
     3841 tatatatctt tcttcgtaat atgaatgtta caatataaaa aactgtctca ataaaataga
     3901 gacagtttta cagtcaaagt ttataataca agttcatatt tttctttaaa ttcttttaca
     3961 gcttcgggcc atgccttttg ttctgtaata cctttttctt tagctatttt aattgcaatc
     4021 tctcttatca ttgcagcgcc ttctaatatt cccattttta atctcccttc aaatatcttt
     4081 ttgtatagaa tataaatagt gctaagattt attacatgct gcatatacaa tatctataca
     4141 acacataact tgtaaattcc ttttattttc atacgtcttt aaccgacata taattatatt
     4201 caataagttt taaaattatg aactcaatta taattatttt tattttttta gaatctaaac
     4261 actaaaaatt gtaggtaaat ttgtcttatg cctttataaa tatttctaca gttttaatta
     4321 ctggacatat ctatttttat tataaaaagc gattaaaaat ggagaatatc ctcataaaaa
     4381 ctatatataa gcaaaacggt taaaggaaaa agaagaaatt tacttgaatg ttaaaatgcg
     4441 ttgcttgaag caatgggcag ttttgcctat aatatcggta acaactgaaa gaaaggtggt
     4501 tagccctaat gttaagcgaa aacattaaaa caatcaggaa gtcaaaagga ctttcacaac
     4561 aagaacttgc tataaaactg aatgtagtgc gacaaacaat atctaaatgg gaacaaggac
     4621 tgtcggttcc cgattctgat atgttgattt ccatatcaga ggtgcttgaa acgcccgtaa
     4681 gcactttact tggagaaact attgttgagt cggaagttga taacttaaaa gtgatttctg
     4741 aaaaattaga gattataaac ttgcaacttg cacaaagaaa aaacatgaga agaaaaatgt
     4801 ttcattggtt atttatttta ttatgtgcag tcatattaat agtttttgtg gtcttaggga
     4861 gactaaatag tccttacttg gattggaatt ataccgaccc tgaaaccgct gtttttggag
     4921 tagcttttca tgcttttgag tggctatttg tcagattagt accgattgtc cttataggag
     4981 caattattgg aatctttttg acacgtaaga aaatatgaag taactctgct ttttataata
     5041 cagcaagcgt tccttaaaag cgtcattcta catggcaatc gaggacgact atgtaaggaa
     5101 gaaccttttt gatttccaac taagcgaagt tattgaggac gacagtaggc ccaaaaccgc
     5161 attaagtaaa gtacaggaag caagactgct ggacttatac tctcatgcgt cagatgcagg
     5221 aaatgcgagg tctggtagca tgatttacaa cgatttgtac aacgactacg agtagaaata
     5281 tatggagata agtattatct acgaaatcag aaaagaaatg acttgataat ttactcatgt
     5341 tataattatt gatgtgaaat taataaatcg aaattccttt tttggattta acaatactga
     5401 gtaaggaaaa agtgaattac ttttgcaagg aggtgttatt tggatggtgc tagcaggtat
     5461 tttgattgta gtacaaggct ttttgactgg tcctttagca aaaaaaattg gtgagaagcg
     5521 actaatactg ataggtatgt tttgcaaagc cttagtagcg tttgccatgg tattgacaac
     5581 tgggtttgtt agtgtgttgg tggtttttgg cttatttgcg gtatcttctg ctattacagt
     5641 acccacatta aacgcaaccc tttcaaaaac tgacaatcag cataaaggaa cactgatggg
     5701 atttgccagt accgccggaa atcttagcaa agttattgct cctttattaa ctgggtatct
     5761 gtatgaaagc aatattgaat taccatatat aaccacggga gggatagcct taatcggagt
     5821 ggttatctgc tatatctgga taaaaaatca aaagaacaaa tgatggaaga ggacgtttat
     5881 atggaggtta tacacagtaa ctaatctggt atagaaattc aattgaggct tttctgcttt
     5941 attaaagtct tgctactact taaccctata atatccttaa ttcattatga caaattccaa
     6001 ttcaacagtt ttgatacaca taaggaggta acaatactat ggaaaattat caatgaagtg
     6061 ctaattcttt atcagtgaag ttatccataa gtttttctac ctgtaatcgt ctggtgcttt
     6121 ttgccagatc attggtaggt aagaaaaatt catcaacaga cacatgaagt aatgatacaa
     6181 ggtcacttgt atgcttggat gttgcccttt attttcaata ttagttaagt aacgtgggtc
     6241 aatatttgta attcgatttt agggaaatat ttaaagaaat attttcgttt tatttcttta
     6301 aatcttcaag aatattgata gttttttgaa agtaggtgtg tattatgaag aatgagaaaa
     6361 aaattacagt cgcattgctc attgtttatt tattgatttt atcatggatt attttattta
     6421 agctacaatt ttcattttct gcgttagacc acataagaca aataaactta attccttttg
     6481 gtggttcagt tattgcaaat ggaaaaattg acttcgatga aattataaac aatgccattg
     6541 tttttatccc tgtaggtgca tatttcagtt tgctttttaa aaataaatct gttctaaaag
     6601 caataggttc tgttttagga attagctttg tatatgagat aattcaattt atatttgcta
     6661 tcggagcaag tgatattaca gacttaatta gcaacacatt agggggtata attggaattg
     6721 cattagtata tgtattatct attgttctaa aggataaaac acataagata ttaaatcgaa
     6781 tagcaatagt atgtactgta ttagtagttg ccttcttatt tatgcttctt gctgtaaata
     6841 atatatttta atgccatgtt aattacatgt tgttttggaa aagactttaa tctgtcaaag
     6901 tttgcttttg aaattgaaca tgaaattaca ccaatggaac aaatgaaagc tataaatttt
     6961 tcattggaga aagatgactg tacaagtaaa gtattaatat acaatccaga caaatggaaa
     7021 tatgtagaat actatttcta taaagataga cctattaaaa aaactattgg aaaaatttat
     7081 tcaattttgc atttgtcaca ataaaatatg ttttgaaata ttgaatactt aaatatgcaa
     7141 aaaaacaaat gtaactggct atggggctat atctactaaa aagctatgtg ataggttggg
     7201 attaaattta aacatcactt ctagaaggca tgaatacaca aggctgacaa actatacagt
     7261 tatgattaaa cgataccaac gggattgaaa atttcgtgaa gaactttgag aagtcctatt
     7321 acacatgagc aacagcaacg aagctattga agcaaggacg caggcaatct agcggttatc
     7381 tgacaaagga gttgtaggac ttgaatgtag atactatcag aacggatata ccgaccagct
     7441 ctgcggttac agatgtgatt gtgtagaata tcgagcagtc gggaacggac actttatctg
     7501 ctacctacga agtagatcag cagataaaag agagagaaca gacaacagcg gtcaaggcaa
     7561 cctataccgt aaaagtccat gtggacgctg acggggatat ggtaatcgtt cagaacctta
     7621 ctcttgcacc agcaatcgaa aaatcagact atgagccaaa gatatcagaa acagacgcaa
     7681 gcgtggacgc tgataccgtt aatgacgcta ccgcatttct ggaaacattc tttgaggtgt
     7741 acccgacagc cacagaaaaa gaacttgcct attatgtatc ggaaaatgtg attgaaccta
     7801 tcggcaggga ctacctttat tctgaattgg taaacccagt ctttacaaag gacggggaca
     7861 atgtgaaagt caaggtagct gtgaaattcc ttgataatca gacaaaggca acgcaggtgt
     7921 cgcagtatga tcttgtactc caaaaggata gcaactggaa gattttagga taacatggaa
     7981 atgggcttgt atcataatta tagtgtgcaa gcccttttca tattgaagta tcaataatca
     8041 ataattataa ttaggtacat gaatggaggg ataaaatgtt caaaattggt gagttttcaa
     8101 aattgacaca agtttctact cgtatgttgc gttattatga tgaaacgggt ttattaaaac
     8161 ctgctaaaat tgattcatta actggatatc gtatgtattc agcagaacaa atctcaactt
     8221 taaatagaat tatatattta cgtgatagtg gcttcaatgt cgctgaaatt gcattagccc
     8281 tcaatttcag taataatgcc attatagaac agcttgatag aaagtctatt gaaattatgg
     8341 ataatataca aaaagaacaa gaaaaactaa agaaaattga gattgctaaa aaagaagtgt
     8401 tacatggcaa aagtgaattg tattttaaca taaccattaa agagataccg agttattgcg
     8461 ttttatcttt aagaaaagaa ataccaaatt attattatga gggcgaactc tggaaagaac
     8521 tctctgcttt tgctaaagaa aataaaataa atatttctga tgaaacattc tcaatatatc
     8581 acaatgaaga atataaagaa aaagatgttg atgtagaatt atgtgccatt gtaaataaaa
     8641 taggaaaagc tacaggagat tttaagtttc gttttaccga accagtttct atgatggctt
     8701 gtactatggt atatggagat ttttctaata tttcttatgc ttatcgttca tttgcaaaat
     8761 ggcttcaaga aaacagtcaa tataagatga aaggttcaag ccgtcaaata gttcatcgtg
     8821 gaccgtggaa cgagaaaaat ccagagaact atctaacaga aatccaaatt cctttagaaa
     8881 atctcttgta aaaaatgata atctgccttg actttaacat ggtgttaagg tttatcgttt
     8941 atttatcaag aagaaaacgg aggaaaatat aatgacaact tataaagtaa attcaatagg
     9001 tactatctgc aacaatgaaa aaggtgcttt cattcaacta gaagcaccat atattctagc
     9061 attaagggca ttagacggat tcagtcatat aaatgtttta tggtggttca gtgattttga
     9121 taatacgaat tttagaagtg tattggaaac agaacagcct tataaaaatg ctccagagaa
     9181 aatggggatt tttgcaacaa ggtcgccagt acgcccaaat ccgattgctt tatcagcagt
     9241 agaaattatc gatattgatt atatgagtgg gctgatacaa attgcttata ttgacgctaa
     9301 tgacaaaacg cctatcttgg atattaaacc atacacgccg agtttggata gagtggaaaa
     9361 tccgcaagtt ccagattggt gtagtcattg gcctcacagc ttagaagaat ctgcttcttt
     9421 tgcatgggaa agcgaattca atttttaagg agtgatgaac catgagtttg caggactaca
     9481 tttttgaaaa accagtaatt gaaacagaac atcttatttt aagaacaatg aataaaaatg
     9541 acatagatga tttgaaagaa tggattagtg atatttcttt atacgagtat tgggggaaac
     9601 gtccctcaaa aagcgaaaaa aatccagaat tactctttga taagtcaaaa aaatctacta
     9661 aaagttttca ttggggtatt gtccataaac aagatagcaa agtgattggt gaaatatgga
     9721 tatatcttat tgaaaataat agaatggcaa aagtggcttt cagactttct aagcattatc
     9781 aaggtaatgg actaatgagt gaagtgttga aaaacgtaat tgaattttgt ttttcaaaaa
     9841 cagaattaca aaaaatatgg acagatgtgc atattttaaa tcatgcttct tacaagacat
     9901 tggagaaagt cggttttcaa agggaaggac atattcgagg ggggaaaatg gtaaatacct
     9961 attgcgacta ctacctatat ggaattttaa aatctgatta tatcactagg tggtgctatg
    10021 gctaatattg aagcgacgat tggcgaagca atgaacaata cagttccaaa ggtctatgat
    10081 acgaaaagca ttaaaagata tgtgatgaat ttattagata tggctcatta aaaagtacca
    10141 aagaaataaa agttaccata tagacaaaat tggtttttta tatcttttga tttcttataa
    10201 gataatggca ttgtgcgaca agctatgcaa aaaattttca tgtatacttt gattataatt
    10261 tatacaagga ggtactgata tgtacgcata tgaaaatatt gaaatgtcat taaactatat
    10321 tgaacaacat ttaagtgaga aaatcgaaac agagaaactg gcggaaattg cctgtctttc
    10381 tacattttat taccaacgct tgtttaaaag gttagttaag aaatcagttc aagagtatat
    10441 caaacttcgc cgtttagcaa tggtaatagc agaattgaat aattcagagg aaagaatatt
    10501 agacatagct ttaaaatatg gattttcaga ccatgctaac ttcacaagag catttaaaga
    10561 agtttaccat attactcccg atgaatacag aaagaatgtt cctatgttaa atacatttga
    10621 caagccagaa ctttcttcac gttatatcat gattgatgaa aatgtcccat tgattgtagg
    10681 aaatattgtt ttggaaattc aaagaaaaac attaagaaca aaggaaacat attttggttt
    10741 tgaaaaaaca gtaaatattt ctgaacaaat tcctgctgga gaaagtacag gaattgatgt
    10801 tccaggtcag ctatggcgcg aattccatgc aaagaaactg caaaccgaaa atgccatttt
    10861 taacaatgat attgaacttg gtgtatctca cttagcaagt ccagataaag gaacattcct
    10921 ttactttgca ggaggtatgg tttcatcaac agatgtatgt cctaaaaata tggtacaaca
    10981 tacattacca gcaggtgagt atattgtatg caaaattgag gcagaatctt ttgaagaact
    11041 tgttactact gctttaaatc aagcaaataa gtatctgttt gaaacttggc ttccaaatca
    11101 taatttggtt acaaagccat tcatggcaga aaaatattac aaaggaaatg cagagattaa
    11161 ttatatggaa atatggataa ttcctgtgta aacagtaaat gctaaatagg aattatctga
    11221 cgtaataaaa aggaaataga aagcttataa aggagggcat agtccgttgt gataaacata
    11281 aaagatataa attatgtacc taatattagt gaaataagcg tttatattga aaactctgca
    11341 tttgatgagt tgtatcagca tatgaacaat gaatataaag cagtttgcaa aatcgaatac
    11401 agtaaagatg tctgggcacg aggttggaac gtaaagttta aaaaatctgg gaaatcattg
    11461 tgtgttgtct atccgaagaa gaactacttt actgtattgg tggttgtagg taataaagag
    11521 aaagaagatg ttgaaaactt gttacctagg ctttctgaat atgtacagga aatttatcgt
    11581 aacactagag agggaaatgg acaaaggtgg cttatgatag acttaaaatc taaaaataat
    11641 gtatatcaag atgtattaag gcttattgaa attcgttatg ccataaaatg tgtaaaaccg
    11701 taataaaaat atttttcaca tacgtaatct caaggtatta tcgttgagac aagtaaccta
    11761 tataattatg ggaaatgaga tttttagata cttaagaaag tactttaggg ggcttgcgtt
    11821 atggaaagat ttaagtacaa ttccacacaa tcaaatacaa tctaataaaa ctttactaaa
    11881 aattgaatgc aatacagctt taaaggctcg tacaaactat atgttttgtg cgggcttttt
    11941 tcatttaata ggaatttgga ttcgtcagag agtatattat aaaaataaaa cgataagggt
    12001 accttaaaaa taaggtacct caggctgtag acaaagtccc ggactctcgt ctgggctttt
    12061 cttatatttg agttatccac aattcaactt tttaagagtt atcttattaa gaaaaaagtg
    12121 tatgttttcc accctatagt tttacttctg tgatatttgc agatgccact caagtagaaa
    12181 gtggtggtac ggcagaagat gtcatgcaga gttcagaaag tctgggacta ccgcctaatt
    12241 ccttagacac ggaaagctca atcaagcagg ggtgtaagta ttttgcgtcc ctgctttctt
    12301 cctgcaaaaa tcaaggtatc gacgatttga atgtagcgat acaatcttac aactatggcg
    12361 ggggttatgt gggctatgtg gcaggaaaag ggaaaaagca cacttttaat cttgcagaga
    12421 gcttcgcccg tgagaaatcg ggtggaaaga aagtaaccta cgccaacccg atagccgttg
    12481 caaagaacgg gggctggcgg tatggctatg gaaatatgtt ttatgtggaa ttagtcaatc
    12541 agtatttaac tgttccgcag gtgtcggggg aactggcaca aaaggtaatg aatgaagcgt
    12601 tgaagtatca agggtggaaa tatgtgtttg ggggaagcaa tcccaacact tcctttgatt
    12661 gtagcggact tgtgcaatgg tgctatggaa aagctggtat ctacttaccg agaacggcac
    12721 aaacacagta tgacgcaacc cagcatatcc ctttatcgca ggcaaaagct ggggagttgt
    12781 tctttttcca ttcaacctat aacgctggtt cgtatgtaac
//
LOCUS       DAMJWS010000101        12792 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMJWS010000101 DAMJWS010000000
VERSION     DAMJWS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.105"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            176..364
                     /locus_tag="PC399_003220"
     CDS             176..364
                     /locus_tag="PC399_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF2594050.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(524..676)
                     /locus_tag="PC399_003221"
     CDS             complement(524..676)
                     /locus_tag="PC399_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF2594051.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(774..1250)
                     /locus_tag="PC399_003222"
     CDS             complement(774..1250)
                     /locus_tag="PC399_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2594052.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1469..2230
                     /locus_tag="PC399_003223"
     CDS             1469..2230
                     /locus_tag="PC399_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2594053.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2490..4418)
                     /locus_tag="PC399_003224"
     CDS             complement(2490..4418)
                     /locus_tag="PC399_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2594054.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4652..5104
                     /locus_tag="PC399_003225"
     CDS             4652..5104
                     /locus_tag="PC399_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2594055.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTNEVKNASDASHLVKYITNKMKEE"
     gene            5107..5415
                     /locus_tag="PC399_003226"
     CDS             5107..5415
                     /locus_tag="PC399_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2594056.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5529..6635
                     /locus_tag="PC399_003227"
     CDS             5529..6635
                     /locus_tag="PC399_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2594057.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6710..9388
                     /gene="mngB"
                     /locus_tag="PC399_003228"
     CDS             6710..9388
                     /gene="mngB"
                     /locus_tag="PC399_003228"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2594058.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9705..12038)
                     /locus_tag="PC399_003229"
     CDS             complement(9705..12038)
                     /locus_tag="PC399_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2594059.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(12013..12729)
                     /locus_tag="PC399_003230"
     CDS             complement(12013..12729)
                     /locus_tag="PC399_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2594060.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4786 a         1783 c         1661 g         4562 t
ORIGIN      
        1 tttccaaata tatttacttt atataaaatt atatatataa ttttatatga taataagaaa
       61 ttcttcaata tgtgtaaggc aaatatatat ttctattgca aatttttgtt atttatgata
      121 atataaatat gtttttagtc tttaaatagt tatcatttta aggaggtgtg cgtagatggc
      181 aaaagtatgt agcgtatgtg gtaaaggtaa ggtttctgga aaccaagtat cacactcaaa
      241 taaacataat aaaagaacat ggtcagctaa cttaagaagc gtaagagcga ttatagatgg
      301 agctcctaag agagtaaaag tttgtactag atgtttacgt tctggtaaaa ttgaaagagc
      361 ttagtaacca cgtttaaaag cctacttccg taagtaggct ttttaattta atcattttta
      421 ctctttctca taaattttag gattatactt gaaatccatt ttggtaattt tattgttact
      481 actttcatca atactcaccc cacactttat agttaaaact attttaacaa aaaaataata
      541 atgctccaca tccaataagt attaatgcgg taagtgctga ccaaatccag ccaggaagca
      601 acattgacag aattacagcc attcctgttg ctagacaaag tattcctagc aataccttag
      661 acttgttatt tctcatggaa cacctccaca tagaacctgt atctataaat attttcatat
      721 tcaagtttat gcgacagttt tccattttag accaataaac acattatgta attttaattt
      781 ttcatactag aattattact gaactccgat atagattcta caatatccaa aattcctttt
      841 tttctactta acctttcagg tccactatat aaaacaactg gtataacttt aggaagttta
      901 aaatttttat cttctatttc ttcaaggctt tttttcataa taaaactttt ccaaatttgt
      961 tctctatacg atttcaatct tgggacaatt tcagtatcca tttctgtttg aaattctaac
     1021 actatataat gtgatacttt gccactttta gaaaatacct tataaattat atcaacttct
     1081 tttcctttat attcacatat aaaagatgtt tctaactcta tatttttaag agttaattct
     1141 tttgctattg agacacttgt aaaattttga atcaaactta aaaaactttc tttatctgaa
     1201 tacattcttc tgtactcttc tcgtttttca ttgcttcttt ttaaattcat acagttcctc
     1261 ctagactata gaccctctat tttttagtaa agatagtcta taaattttat tttttaattt
     1321 tcattagtga gtattgacag ttgtgtgtaa aatatccagt aaagatattt ttttatattt
     1381 ttattaaaag atataaattt ttatatttta atgtattgac atttacgttg cgtaaaagtg
     1441 tatatttaat ttataggagg tactacttat ggaatataca gttcaaaaat taagtaaact
     1501 agctggtata agtactagaa cacttagata ttatgatgaa ataggtcttc ttaaaccact
     1561 taaaataaat tcttcaggtt atcgtatata tggtcaaaat gaagttaata aacttcaaca
     1621 aatacttttt tatagagaat taggaattaa cttagaaaat ataaaaaaca ttataaattc
     1681 accaaacttc gatagtttaa gtgcgttaaa agaacaccat aataaattac tagccaaaag
     1741 aaagcaaatt gatttactaa tagaaaatgt aactaaaact atagctctga aggagggaaa
     1801 atacactatg acagatatag aaaaatttga aggttttaaa gaaaagatga ttgatgaaaa
     1861 tgaaaaaaat tatggaactg aaattagaga gaaatatggt aaagatatta tagatacatc
     1921 aaataaaaaa cttaaaaata tgtctaagca agattatgaa aattggcaaa acctaaatat
     1981 cgaaataatt agtaaactta aaaaagcttt taaaactggt gatgcctcta gtgaactggc
     2041 tcaggaagtt gcaaaattac atcataaatg gttgagttac acttggaaca catactctaa
     2101 agaagctcat tcctctctag cacagatgta tgtatatgat gaacgtttta catcatatta
     2161 tgatagagaa caacctggat tagctaagtt tttaagggat gctatagtaa tatatacaag
     2221 agataaataa tataaaataa taaaggtggt gtctcaaaat gaacttttta gttcatgaga
     2281 cactcttttt tgtatgaaat cagatatatt tttatcattt tatcaatgaa aaacaggttt
     2341 ctctctctat tttgagacag aatctttatt gtataataat tataatcaat acaaaataat
     2401 aacaaattat acaaaactta cttatactat ttatgctaaa aacaaaaaat attttattaa
     2461 agtttttaaa acttatgtaa tataaaaatc tatactatag acttgattat ggaaagaaaa
     2521 ttatcaaaat cattacaatt agatatacta tctctaatat cattgtcatg aattatcttt
     2581 aaaaggatat cataaatgga gtttattttt atataatcat ctttatttat agccaacatc
     2641 atgattaatt taacagtaga atcttcactc caatatattg gttcatccaa tatggaaata
     2701 caaatcttag ttcttataga gcttaattcc ataggatgag gtatagctaa aaaatcatct
     2761 atattggtag aagataaact ttctctgtaa aatacagatt cagcaaatga tggaaataca
     2821 atttcacttt gagttaattt gttgcacatc aactttataa gctcatcttt atctttgatt
     2881 ttaggattaa caataaataa atttttatca aagaaattgt ccaaaagatt actataaata
     2941 tgggaattat tagtgattga cttagatata ttctctatat ctttttttaa tagttttaaa
     3001 tccactaata caataggaat actatcatga tttaaaggaa ttgtcgatat tattatatct
     3061 acatctgaca attcactagc taaaaattga ttaaatgata gacattgaag tatatttatt
     3121 ttatcatgaa aaactttgtt taattgaacc tctaataacc tacttgaacc ataccctgaa
     3181 ccacatacta atactacgtt tttacactta atattacttt gaaaaaatct ctctattcca
     3241 gcacctatat gcagggatac atatccaatt tcatcttctg ttaaagagta aatactattt
     3301 ttaaaaactt tttctatagc atttaaagtt atttcaaatg ctaaaggata attactcttt
     3361 attgtattta taaggggatt caccttattt aaattataag atttagaatt gagaattgat
     3421 ttaaagtgca gtactaaatc atcaaataat acactatcat ttctaaggtc aaaagtatat
     3481 tgattgttga ttacttctaa aatttcttca acatactcta taattttagt atctacccta
     3541 ttggaaacgt tatccaaaag agaactttta gttataaaat gattatatag ataaactctg
     3601 tctgcctttg aaataattat agtatattta ctctcaatat aattaaatat attttcaaca
     3661 gttgtattcg attgaaaatc tgttaaaaca acatctaaaa cattatctaa gcagtaacca
     3721 tcaagtattc tacttacagt aatagctaaa tggataataa agttttttag attaaaatct
     3781 aggaaattga tattaaattt attaatttca cttaacacta ccctttgaag ctctattaaa
     3841 tctacattat taaataattc tatttctcta tctgtaattc caattatata gttttcaaaa
     3901 ttcctattta tcatattatt agaaatacat cttcgaatat ctatttcctt accttcaact
     3961 ttaactccat aatatggctt agaaactaat attaaattat actttgacaa aataggcttt
     4021 attcttttta tgtcatttag tattgtaaat ttactcacat acaagctatt agctaaatct
     4081 tctagtttta tatatgtatt gttatagaga atatatttta aaatatattg atttctttct
     4141 ataggagaat ctggtatttc acttgaatcc ataatatcat cagatattaa tgctagatac
     4201 tgttggtata atgctaaatt aagaatatct atataatatc cttctccttt ttttatttta
     4261 ataatagcac catttttctc taaagttcca tttatagaag taatatcatt tcttatagtt
     4321 ctctcactca cttttaaatg attacttaaa gttttacaag taattttatc atcactttca
     4381 tttaataatt taaacaacgt gagcaatctc ttgtgcataa atatcccccc ttatagaatt
     4441 tcttctaaat tatactattt acactcatat aatacaatta ctctaataaa attgtattat
     4501 aataatttat ttccacttct atatggaaat tagttattat cataatataa atttccatgt
     4561 cgaagtggaa attagttata tttaatttaa ttaggataat gattacaata taaatataag
     4621 ttaagacatt atgaatggag tggaaaaata tatggtacta caagaaaaat atatatttat
     4681 agataaagac ttaaaaagca aagaagaagt attaaatttt attgcagata gtgcttatat
     4741 actaggtatt acaactaata aagaggctct tttaaaagac cttctaaaaa gagaaaatga
     4801 atttccaaca ggaataaaag atggatttgc gatacctcat acaaaaagtg taaatgtaaa
     4861 ggaagcttcc atactctata ttaaaacaac taacgagtta gagtggaaaa ctttagatga
     4921 tagtaaggtt agatatgtat ttagtttgtt agccccacat aaaaatgaag gaaatataca
     4981 tttgaaaact ctatctaaat tagccacatg tttaatggag gatgatttta caaatgaagt
     5041 taaaaatgct agtgatgcaa gtcatttagt aaaatatata actaataaaa tgaaggagga
     5101 ataattatga aaatagtagc aataacatca tgtatagcag gattagcaca cacacctatg
     5161 gcagcaaaag ctttagaaca agctgcatca aaaatgggtc atgaaatcaa agtagaacaa
     5221 caaggtgcta tgggacgagt aaatactata acaaaagaag aagcacaaga tgcggacttt
     5281 atacttatag catctgacca aactatatct ggaatggaaa gatttgatgg taaaaaggtg
     5341 ataaaagtca aaataggtat agcgttaaaa aaaccagaag ctgtcttaac aaagtgtata
     5401 gaagctattt cttaataaaa aaatctcact ctaatataga atgagaaaaa tcaaaaatta
     5461 aaaaattgtc taatgtaatt ataacatatt atcaaaaaat ataacaaact tatataggag
     5521 gattaaaaat gagtaaaaaa aataaatctc ttttaagtga acttaaggga cacttaatga
     5581 caggtatctc atatatgttg ccacttgtaa taggctcatc tttagttgta gctatcccaa
     5641 agcttacagc tttagcaatg ggtataacaa gtttagatgc ttataaaaat gtatctggtt
     5701 tttatcatat tctttactta atggaacaag ttggttggac tggcataggt ctattaaata
     5761 cggtattagc aggatttata gcattttcaa taggtgagaa accagctatg ggtgcaggtt
     5821 ttattggtgg gctcatagca agtaatacta atgcaggatt cttaggagca gtaatagcag
     5881 gtttctttgc tgggtatgtt tgtaagtttt taaagaaaaa aattaaaata tctgggtcag
     5941 catcaggaat gatgccactt ataataatgc cacttataac agttggatta actggttttt
     6001 taatgagtgt tgtattagca ggacctttag gaaacataaa tacttcatta aatgcttggg
     6061 tagctgaaat gtgtcaaaat ggaacaaatt cggtggtatt ggctttaatt ttaggcgcaa
     6121 tgataggatt cgatttaggt ggaccagtaa ataaagcagc ttggatggca ggaaatgcat
     6181 tattgttaga gggaatctat ctaccagcga tattggtaaa ctgcgcaata gttatacctc
     6241 cactaggata tggtttagct acattcataa gaaaatcaaa gttttccaat gcattagctg
     6301 aaacaggaaa aggcaatata gttatgggta taattggaat aacagaaggt gcaattcctt
     6361 tcactttaac aaatccatta aaattagttc ctgttaatat gataggatgc gctttgggtg
     6421 taggattatc agctttgtta ggtgtacatg ctataatgcc tccagttggt ggattatatg
     6481 gatttatatc aataggttct ggatgggctt acttagtagg tgcaatattt ggagctttag
     6541 taatagcaat attatcaaca actttagtag acttcaatga agatgaaact acggaaaata
     6601 cagaatttga tgaaatagag ttagatataa tgtaaattaa aaacttaatt ttagataatt
     6661 gagaattgag tgtactctca attctcaatt aaattttggg aggattttta tgaaaaagac
     6721 aaaagtacat gtagttccac acagtcattg ggatagagaa tggtatttta caacatcaag
     6781 gtcaaaaata tatttaatga aagattttca agatatatta gacatattgg aagaaaaaga
     6841 agactttaaa tactttacta tggatgcaca agcttctctc ttagatgatt atttaaaatg
     6901 gagacctcag gatgaatata gaataagaga tttagttcaa agaagaagat tagttattgg
     6961 accttggtat actcaaacag accaattggt tatatcagga gaatctataa ttagaaacct
     7021 ttactatgga atagatagat gcaatgaatt tggtgaacct atgagggttg gatatgtacc
     7081 agattctttt gggcaatcag ctcaaatgcc acaaatatac agaggttttg gaatagatag
     7141 ttcattattt tggcgtggag tttctgatga tatggtagat acaacagagt ttatatggaa
     7201 aggttctgat ggttcaaaag tattagcagt tcaaatacca tttggttatt actatggagg
     7261 taatatacca gaagatgatt ctgatttaaa agaatattta aaagatataa tcggaaaact
     7321 taaggttaaa gcttctacta ataacgtata ttttccaaat ggatttgacc aagcaccaat
     7381 aagaaaaaac cttcctgata tattaaaaaa agctaatgaa atagataatg aaaatgaata
     7441 cgaaataagt gatatagaaa actatataga ttcagtaaga aatgaaagaa atgaatttac
     7501 agagcttaaa ggtgaatttt taaatgctaa acatatgaga atacacaaat ccatattctc
     7561 ctcaaggtca gaccttaaaa taatgaataa caaattagaa aattatatta caaatgtact
     7621 tgaaccatta ttaacattaa gttattcact tggaaatgaa taccctcacc tagttataaa
     7681 agatatatgg aaacttatgt ttgaaaatgc agctcatgac agcataggtt cttgtaatag
     7741 tgacacaaca aatgaggatg tatacacaag atacaaacaa gccagagatt tgagtataaa
     7801 tttattagat ttgcatatga gacttataag tactcaaatc aaaaataata atgaagaaat
     7861 aacattaaca gtattcaatg gattcccaga agtcagaaat gaagttgtag aatttgatac
     7921 ttatattcca ggtcaagatt ttgtgataaa gaataaaaat ggaaaaattc ttaattatat
     7981 aataaaagaa aaagaagata ttacaaatta tatattaact caaacaataa gattaaaccc
     8041 aagtaaaaaa atatatatac ccaaaactgt atatagagca aaaattgcac tttcagtaaa
     8101 agatatacca ccaatgggat atactcaact agtttttgaa ttggatacta aaaataaatg
     8161 tgaaatagaa acaagtaatt gcaaggaaat agaaaacaaa tactacaaaa taacagccaa
     8221 taaaaatggt tcattagaaa tattagataa agaatctaat aagacttata aaaaccaagc
     8281 tgtaattgaa gacaatggag atgatggaga ttcatacaac tattctcctc caagaaaaga
     8341 tatttatgtt tcatctttag attctgtatc ttcagtagaa gtgctaaaat caaacattca
     8401 agaagaaatg atacttaaat attcattgac cataccaact tcactggaag aaagggctat
     8461 aggtaaagtt tctgtgaaaa tgccagtaac aatgaaaatc accttagaac ctaaagaaca
     8521 aataattaag tttaatgtta atataaaaaa taaccaagca ctatctcata gagtaagagt
     8581 tttatttaat acagaaatag catctaagtt ttctatagca gatcaacaat ttggaataat
     8641 acaaagacca acagtattag aaaaagacct tgcattgtgg aaaagagaca attactcatg
     8701 gcaagaaaaa cctataacta tcgaaccaat gcaaagtttt gttactttag aggatggaca
     8761 aagaggaata gcattaataa ctggttgtgt aagagaatat caaatagttg gtgataattt
     8821 agatacaata gcattaacac tattcaggtc atttggatat atgggaagag aaaatttact
     8881 ttatagacca ggaagagctt ctggagaaaa aataatagca acaccagatg cacaattact
     8941 taaagagtta aactttgact ttggtcttta catatataac aataaatttg atgaagcaaa
     9001 tgtagctaat acagctaaga gattcttaac acctatacaa atatatgaat atgctgattt
     9061 tttaaatggt agacttatat ttgcattcaa tgacgaaaaa caaatatatg aaaatgaatt
     9121 tagtttgatg aatgtaaata attcaaattt cacagtaagt gcaataaaga aagaagaaaa
     9181 aggagatgga attgtagtta gaatcttcaa tggtatgaaa aatgaggatg ctaagggaag
     9241 tcttaacatc aataaaaatg tcaatgatgc ttatagtgca atgctcgacg aaatctatga
     9301 taatactagc aagcttaaag taaatactaa aactgtagag gtaaattctt tatcacattg
     9361 taaagtacaa acaatagtaa ttaaataata taaaatagct tatacaagat ttcaaaataa
     9421 attgctagta ctatgttaat agtaaagcca gatattatgc tatttataag ctctgtaaca
     9481 ctaaataaca ttttgtaaaa taaaaaggtg aaccttgtat attaggattc acctttttta
     9541 aataaatcaa cattctgttt taaaataaca tttataagct gttttgaaat aatgcttaca
     9601 aattatttta aaacaatacc tataaatttt tatttaaaga atacacataa atatttccag
     9661 attcttttat tctatagact ttataattat taaagaagtc taatctaatt tttgttaaaa
     9721 gtaattgtaa ctttgaataa atcaccatcc acacttatat tcatcttacc accctgtaat
     9781 tctataaaac tttttgctat cgctaaacca agaccagaac cttctgtgtt acgtgattta
     9841 tctcctctta caaatctctc cattatatca tctacactaa aatcaatctc agatgcagac
     9901 atatttttaa gcataatatt tactttatta tctgtttcta aaatatcaat atacactctt
     9961 gaatttggca ttgcatattt gcttatattt attataagat tttcaaatac tctaaatgta
    10021 cgctgactat ctaatggtaa tattattttt tcattagaaa agttattctt tactattaga
    10081 gatgaatctg caagtttgtc atctaattct aaaagtgtct gcttcattag agatacaata
    10141 tccacattta ctatattcaa ataaacatct ccactatttg ccttgcttac ttcaaacaaa
    10201 tcttctatta gatgttgcaa tctttgtgac tttctgtcta atgtatcaat atacaactta
    10261 cgattttcat ctgttatact ttcatctttt aataaatcta tataagtgat tatagatgtt
    10321 agaggtgttt ttaagtcatg tgatacattt gatataagct ctgttttcat cttttgactc
    10381 ttaacctctt cgtcaacagc ttttttaaat ccagtttgaa tatttcctaa ttcatctttt
    10441 ataggattaa aaaatcctaa atctttttcc atatttacat ctaagtttcc atctgaaatt
    10501 ttctttgtaa catctaaaag ttgattatat tcatatattg tctttttaga gtaatctgac
    10561 agtatcttaa ataatactac tgaatatatt aatgcgagta ttattccaaa gaaccacata
    10621 ctacacatta taactataat caataaattt attcccaaaa ttgtaactat agttttattt
    10681 ggcttttctt tcagatttat tgttctaatt tgattaataa ctgcttttcc tgcttttttt
    10741 aaccatacaa aaaacattcc tgtcacagta tgttttgtaa aatactctct tattccaaca
    10801 tttaaaatgt gttttaaaag tattataaat acaaatatta ttgagaatat tacaaaccaa
    10861 taagttaaat tcattaaact taccaatata ctagccattt tagcacttat gtcattgttt
    10921 ataaggtact ctacataatt cccactcaag gtattactta ttataacagt aggatataaa
    10981 tctacaagcc cctgtataat agcaagagca ataaagagta tctctaacgg tattcttgat
    11041 aatgtatcaa atcctagcaa ctctttagat tttctatatg ggaatgctaa agctacaata
    11101 acaactatag ctaatataat ttttaaaatc attccagaaa tcttataata agaattattt
    11161 tctacattgc ttatatatga aattatacca tcatcaaaat aagtatttcc ataaccatac
    11221 tctacactat cacctttact tattacttta ggtacagcat atactaatgt tatatttttt
    11281 attggagata acttatatga aattgaattt cctcctaaac ttgtatccat taaatcttct
    11341 cttgctttag ataacaacat ttgattaata attttttgat ttactccata agaatccaca
    11401 atatttactt ttccatcttt atcaaaattc attacaacgt agaatctata gttttctttt
    11461 atatctgtaa tctgcttttg tctttcctca tcattcattt ctgtattttt tgactgattt
    11521 tcattagaat tttcttttga agcatcattt tttgttgtat caagtactcc taattcttta
    11581 ttatcattaa aaaattcaaa attgctttct ttatcaacta aatagtactt taaattcttt
    11641 cctgacgctt taatgaaatg ctcccaattt tcaaagtttt cattaaaatc tgcattaata
    11701 ctatcatctg tataataagt ttctgaacca tcataagtat ctttttcttt acttacattt
    11761 tttatatagt tcatagtttc ttcattgata aatagctttg atggttgtat catcttacct
    11821 tcttgttctt ccttcatatt atgatataaa acataacttc ctttatatat actatctaca
    11881 aagctttcct gttcaaatgg attctctgct ccacctttag cacttgcttg tatagtagaa
    11941 tattgactaa tcatacctaa tgatgctgat aacataagta taaaaactat aatagtaact
    12001 atatatttat ctttaaattt tttcaatttt gtatccaaca ccccacacca cctttaaata
    12061 tttaggattt tttgggtcaa tttctatctt ttcccttata ttcctcacat gtaccataac
    12121 tgtatctgta tttacagcat tctcattcca aactctttca tatatttcat cagcagaaaa
    12181 aactcttcct gcatttctca taagtaaact tagtatttta aattctatag gagttgtttt
    12241 tatatacttt ccatcaatac taacttcttt tgtattttca tttaattcta atccgcctat
    12301 tgcaaacaca ctatcgtcac tttctacctt ttgctcttta tttccaacca tgtttagata
    12361 cttagtgtac cttctaagtt gtgaattaac tctagccaat agttctaatg gcttaaaagg
    12421 ttttgttaca taatcgtctg ctcctatatt aagccccatt attttgtcaa cttcttctga
    12481 ttttgcagat agcattatta ctggaaaatt atagttttct ctaagcttca ttgtaagtgt
    12541 tataccatcc attttaggca tcattatatc aactatagct aaatgtattt cttggttttc
    12601 tataacctta agaccttcta atccattgta tcccttaaaa acattatatc cttgatttaa
    12661 tagatatatt tctatagcat ctgctatctc cttttcatct tctaccacca aaatattata
    12721 tgaattcatt ctaatctcct ttatattaat ttataaattt tctttacata tatgattata
    12781 caatatgata ga
//
LOCUS       DAMJWS010000102        12469 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMJWS010000102 DAMJWS010000000
VERSION     DAMJWS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.106"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            253..699
                     /locus_tag="PC399_003231"
     CDS             253..699
                     /locus_tag="PC399_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594061.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVP"
     gene            1080..1997
                     /gene="pstC"
                     /locus_tag="PC399_003232"
     CDS             1080..1997
                     /gene="pstC"
                     /locus_tag="PC399_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2594062.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQGIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            1999..2823
                     /gene="pstA"
                     /locus_tag="PC399_003233"
     CDS             1999..2823
                     /gene="pstA"
                     /locus_tag="PC399_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2594063.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            2842..3606
                     /gene="pstB"
                     /locus_tag="PC399_003234"
     CDS             2842..3606
                     /gene="pstB"
                     /locus_tag="PC399_003234"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2594064.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            3634..4287
                     /gene="phoU"
                     /locus_tag="PC399_003235"
     CDS             3634..4287
                     /gene="phoU"
                     /locus_tag="PC399_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2594065.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            4445..5314
                     /locus_tag="PC399_003236"
     CDS             4445..5314
                     /locus_tag="PC399_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2594066.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            5401..6837
                     /locus_tag="PC399_003237"
     CDS             5401..6837
                     /locus_tag="PC399_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2594067.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            6885..7712
                     /locus_tag="PC399_003238"
     CDS             6885..7712
                     /locus_tag="PC399_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2594068.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    8008..8270
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8346..11012
                     /locus_tag="PC399_003239"
     CDS             8346..11012
                     /locus_tag="PC399_003239"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF2594069.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     gene            11437..12147
                     /gene="rgaR"
                     /locus_tag="PC399_003240"
     CDS             11437..12147
                     /gene="rgaR"
                     /locus_tag="PC399_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF2594070.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNTITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT         4925 a         1297 c         2291 g         3956 t
ORIGIN      
        1 tagttaaata ataagattaa gaatttctta ataaatccag aagatgcttt cgattatata
       61 atattttata tttttatatt attaattata tgtgttgcaa tcgctgcacc tactttttct
      121 ggtgagtatc agacacaatc agatagcatc cttcgttgta cgaaacatgg acatattcgt
      181 ctagcgttta ctaaaatatt agctctattt tctatattta taataatgtt tgttatatgt
      241 atttctatcc atttggcaat ttctgattta gcttttggta ctgaatgttt gaaaacctca
      301 tttcaaatgt tgttttctgt cattagtcta gtaaatataa atttactaca agcacagatt
      361 cttatagcta ttggtggtct aatttcaata cttgcaatgg ttagtttaac attattttta
      421 tctgcaaaat gtaaggattc tcttacttct atgctgattg ctttttctat ttgcttaatt
      481 cctatgggaa taggtgttag ttggattgga tacctattac caccaggggg aattggtttt
      541 aagattagtt tattatatca aatgacatca tttaatttct tacacttagg aggtatgagt
      601 ttttggacac cacacattat actgtttttc tcagtgatag aaatacctat ttttatattt
      661 ttggctattc gtacctattg caaacatcaa gtaccgtaac aatttgatgg aaataatagt
      721 gatataaact agaggataca ataattatat atgtagtata aaagttaaat ttagttaaca
      781 aatcttaata gcagatatag gtaataaatt atacataaac tgtattatta tctatatctg
      841 ctattttaat atagagaaac aacagtatat tgtaaagtaa atgtcaaata tagttttaaa
      901 agtaaaatta atgttaaaaa gtaaatataa ttttacactt gcttaactta atcctaatat
      961 agatttaact taaaaaaagt agaataaaag tataggaaat ttgatgaaga cgaattataa
     1021 aaaattaaat tttgaataca gaacgaaaat cgataagtat attttaatgg gagggtatta
     1081 tgatagtaaa atctagatta gtaggtttag aaaaaaatga caataatggc aataagacaa
     1141 aatacgtaat tgaaaaagta gcaaaaaata tttttctact tagtgcttta gtagcagttg
     1201 caagtttact tttgataata ggattcgtat tttataaagg attaagacca tttatatttg
     1261 aaggatattc atttatagac tttttaactg gtgcagattg ggttccttct gccaataaat
     1321 ttggaatatc atctatgata gtagcatcaa tatttgcaac acttggagca cttgttatag
     1381 gagtacctgt tggtatatta acctctgtat ttatagcaga agtagcacct aaaagattag
     1441 caaaaataat gtcaccagca gttgaacttt tagcaggtat accttcagta ttatatggtg
     1501 tatttggtct tgcagtgata gttccaacta tacaaggtat atttaatctt ccaaaaggtc
     1561 aaagtttgtt agcaatagta atagtattag cagtaatgat gttaccaaca ataatatctg
     1621 tatcagaaac agcaataaga gcagtaccaa aagcatacaa agaaggttca ttagcacttg
     1681 gtgcatcaaa aatagaaaca atattcaaag ttgtattacc tgctgcaaaa tctggagtat
     1741 tagccgcagt agtgttagga gtaggaagag ctttaggtga aacaatggca gtaatacttg
     1801 ttgcaggcaa ctcaccagtt atgccaactg caataacaga tagtgtaaga ccacttacaa
     1861 ctaatatagc acttgaaatg ggatacgcat ttggtacaca tcaagaaatg ctatttgcaa
     1921 ctggagtagt attgtttaca tttatattaa ttttaaactt agttttaaat aaactttcaa
     1981 ataaggcggt aaattaagat gagaaagttc aaagagaatc tattgaagtc attagtatat
     2041 ctatcagcac tatttacaat agtatcatta gtagttatag tgggatttat atttataaag
     2101 ggaattggca acatgaattt taatttctta tttagcaatt attcagcatc aggtgatggt
     2161 ggtatacttc caatgataat tacaacatta tatacagtag tagtttctat agttgtagca
     2221 actcctatag gaatattggc tgctatatat ttacaagaat atgctaaaaa aggaaaagca
     2281 gttacagcta taagatttgc aactgaaagt ttatcaggga taccatctat agtatatgga
     2341 ttatttggag gaattttctt tgtagttaca ttaaaaatgg gatattccat agttgcaggt
     2401 tcacttacag ttgcaataat aatattacca gtaataattc gtacaacaga agaagcttta
     2461 aaaacagttc ctcaagctta tagagaatca tcattggcat taggagctac aaaatttcaa
     2521 actttatata aggtagtact tccaagtgca ataccaggaa ttttatctgg agtaatctta
     2581 tcagttggtc gtataattgg agaatcagct gcaatattac ttacagcagg tactgtagcc
     2641 aagatgccag gtggaatatt tgatagtgca agaacattaa ctgtacactc atacttactg
     2701 actaaagaga gtggagatat agcaacagca gccagtatag gaattgtttt aatagtaatt
     2761 gtattagcac taaacatgct agcaagattt gttgctaaga aattaaataa agctaattat
     2821 taaatttaag aggtgtgaat aatggaatta atcgataaga taaaaatgag tgtaaaagat
     2881 ttggatttat tttatggtga taagcaagca ttaaagaaaa taaatatgga tataaaagag
     2941 aataaggtta ctgctttaat aggtccatct ggatgcggaa aatcaacttt tataagaacg
     3001 ttaaatagaa tgaatgactt aatcgaagat gtaacaatta aaggaaacat ttctgttgat
     3061 ggagaggaca tatatacctc agatgatgtt ataaatcttc gtactaaagt aggaatggta
     3121 tttcaaaagc caaatccatt tcctatgagt atatatgata atgtggcata tggtcctcgt
     3181 acacatggac taagagataa gaagcaatta gataaaattg tagaagaaag cttaaaaggt
     3241 gcagctattt gggatgaggt taaagacagg cttaaatcat ctgcactagg actttctggt
     3301 ggtcaacaac agcgtatatg tatagcaaga gcaattgcta tgagaccaga agttattctt
     3361 atggatgaac ctacatctgc tttagaccca atttcaacat taaaagtaga ggaacttata
     3421 gaggatttaa aaaaagacta cacaatagtc atagttacac ataatatgca acaagctgct
     3481 cgtatatcag atgaaacagc ttttttctta aatggagagg ttatagaatt tagcgataca
     3541 aaaactatgt ttaccactcc tgtagataaa agaacagaag actatataac aggtagattt
     3601 ggttaaaata tataactata aaggagttag acaatggtca ataatagttt agatataagt
     3661 attgatactt taaaagaaca aacagtgctt atgatggaaa aatgtgaaga agcaatagaa
     3721 aagtctgtga cttgtatgat agagaaggat ttagagggtg caagaaaagt agtagaagat
     3781 gatgaagaaa taaataaatt aagagaagaa ataagagata agagtataga gctaatagta
     3841 ttaaagcaac caatggcaag ggacttaaga tttatttatt cattaggtaa tatatctata
     3901 gagctggaaa gaataggaga ttatgcagca aatatagcta gagaaatgtt gaagataggt
     3961 ggagaggctt atataaaaga gctaatagat attcctaaga tggcaaaaga atgtaagtct
     4021 atgatgtctc agactagaga agctttaatc ttatttgatg caagtatagc ttatgatgtt
     4081 ggaactaaag atgatattgt agatggattg tatgaacaag tacaaattga ttgtttaaaa
     4141 ataatgcatg aaaactcaaa taccatagac caaggggtaa aacttctact tataggaaga
     4201 taccttgaaa gaattggaga ccatataaca aatgtatgtg agaagataat ttttgcaatt
     4261 gagggtaaaa tggttgagat aggataaaaa actgctttaa agcagttttt ttattgtcta
     4321 aattaagaaa gaattgataa aaaaatatga ttaatgggta aatttaaaaa attgtgatat
     4381 actaggaaca tattaaatat gaggatgcgt attttatata aggtgctttt taggaggtgg
     4441 aactttgaaa cttcaaaaga tgggtatgag gactataaaa acaggaatta gtgtcacttt
     4501 atgtatgatt cttggtgact atttagtaga gaatatgttt tattctctgg tagcatgtat
     4561 aatttctgtt caagatacag taaaaggttc tttaaagacg ggaatcaata gagtggaagg
     4621 tactatttta ggtggtataa taggattcat atttgcaatc attaaagctg gagacccatt
     4681 tttatgtggt ctaggagtaa tgtgctcaat atatttatgc aatctgttta aaataaagtc
     4741 agtagtagtt gcctgtgtaa cctttctggc tatacatcta ggtgtaggaa atagtgaccc
     4801 tgtgtattat tctttacata gggtgttaga tacatctgtg ggtgttttgt ttggagtact
     4861 tgttaattat tatgttgcta gaccaaatta tctaaatagt gttatagatg agtttaaaaa
     4921 gatagaagaa ctttcaatgt tgcttattga agataaaatt cttagaaaag aaaatttaaa
     4981 tgtagagaaa tttgaagaag aagtagaaaa attagaagaa atatattcaa aacttattga
     5041 tgagttggat tatagtttga gtgaaactaa tatagaaaaa attgagaatg ccttaaggat
     5101 atgtagagaa atacattttc atatgcagtc tatagagtta cttgaaaata aactttattt
     5161 gaaagagcat acatataaat accttaagaa aacatataat gtggataagc tagactggaa
     5221 tatagatgaa gaaaaaagcc ctgtattcaa ctatcatcta atgaaaataa tgattgaaat
     5281 agagttgttg agaaagctaa ataaatcata ttaataatag tagttaaata ataaataaaa
     5341 aatagtatct ttgtaaatat taaacatata taagtttaat ttatggaggt actatttttt
     5401 atgaatacta gaaatgacaa ttctaaaata ctagaaatat ataaaatgaa gctttttggc
     5461 gaacttgtaa aagcagtgtg gaatgatgat atagaggata ttgaaaactt acctaataaa
     5521 attttaaatg aagatttaaa ggataattta aataattatt ttagtggaaa atttgataaa
     5581 gatactatgg taaatttaat tagggtaact atgggattgg atgttacaga agatacagat
     5641 aaaaatttaa aagagtcact gcatgaagct gtaaatttag atgaagtcaa aagaccaata
     5701 atatctgtaa tatcagatgc ttgcaaatat tgtgatgaaa ataataagga agatgagtca
     5761 tgtaagataa gaagtaagca tattaattgt aatgaggaag atgtatgtag tgcttgtggt
     5821 gattgtgtat caagatgtaa gttgggtgca atttcagata aaatagaatt tataccaatg
     5881 ataaaattgt taaaagatat aaagagccct gtatatgcaa tagttgcacc agcttttgta
     5941 ggacagtttg ggaaagatgt aagccctggt aaattaagaa ctgcattaaa aaagatagga
     6001 tttgaagata tgatagaagt tgctttggca gcagatatgc ttactgcaaa agaagcatat
     6061 gattattatg agcatatgaa aaaggacgaa gaaggatgct ttataactag ttgttgctgt
     6121 cctatttggg ttagtttgat acaaaatagt tttcctcaga tattggaaaa tgtatcacca
     6181 tccgtttctc ctatgattgc ttgtgggaga gctgtaaaaa ttttaaatcc agatgctaaa
     6241 gttgtattca taggtccttg tatggcaaaa aagaaagaag caatgttaga agatataaaa
     6301 gatgcagttg attttgtgct tacatttaaa gagttgcaag aaatatttga tgcattagag
     6361 ttaaatccag caataatgga tgagagtaat agagtagagg cgtccctttc aggtagagtt
     6421 tatgctagaa caggaggtgt aagtaaagca gttaagttat cagttagaag aattgacaaa
     6481 aatataaaat ttgaagaaaa ggcatttgaa ggaacaaaag agtgcatgga cggtctaaag
     6541 aaagttctaa ataaagaaat agaagctaca tttatagaag gaatggggtg tgtaggaggc
     6601 tgtgtaggag gtccgaagcg tattttaagt gtggaagaag gtactaagaa tgttgatgaa
     6661 tatagtgaaa gtacgaatat ggaaactcca tttgataatt taaatgtatt acaattttta
     6721 acttctttgg gaatcaaaag agtagaatct ttaggagaaa aagaagaaga aaaggttaaa
     6781 aaaatattta gtagagatat aaaaagtaat aagaaagaga ataaatataa aaaataactt
     6841 tttaagtctt aaacttatgc ttttataaaa gggagatatg ggggatgaaa attaaagata
     6901 ataaggctgt ttttcatgta tttataagga tatttcttat agctgtttta attttaactg
     6961 gaacattatt ggaggaaaaa attaataatg ttccagttga aaatataagt tcaaaaggtg
     7021 aaaatacact ggaactgctt tctcaaagta atactttagg agaaatcata tacaaagcat
     7081 ctcaagaaga aacaaaaggt ccaggggcta cttctgaaaa agtagcatac ataacaatag
     7141 atgatggtcc atcaaaatat acaaatgcac tacttgatat tttaaaagaa aatgatgtca
     7201 aagcgacatt tttcatgctt aatcagaata tgaaaaacca caaggaagaa gtaaggagag
     7261 tcttggaaga agaaaactca ataggttttc atagtgtaag tcatgatata cataagttat
     7321 ataaaagccc agaagtaaca gttggggaat ttaatacatg caataatact ctacaagaaa
     7381 taactggtca gagttcaaaa ttaataagat taccatacgg aagtaaacca tatatgccag
     7441 aaggttcata taataaactt atggcaaatg attatttaat ttgggactgg aatttggata
     7501 cacaggattg gaagtcttct acaagtcaga tagtaagtaa tatattatat tatggaagaa
     7561 aaagagatga attagtagta ttaattcatg agaaagagca aagtttaaat gctttaaata
     7621 atattataag aatcttaaaa gagagaggat atacaatatt gccaattaca gaaaatataa
     7681 cacctaaaaa tttctggcaa gaaaacttat aataaattta aatatagtaa aagataaagt
     7741 aaattattta taaaataaaa catataatat aaaaaatacg aataagaggt atactgtaag
     7801 gataatataa aattaatgag aaagcattaa aattaaaata aaataatctt gcaattattg
     7861 acattataag acagaataac tgtaaaaaca tattttaaaa tataacaact atataaaaga
     7921 gaatagacat aattaagaaa attcagaaaa aataaaataa aaataattga caaagagaat
     7981 aatatttgta tataataata tgtaatatta aagactgaga agaagaggag taagtaaata
     8041 ctactaaaag agaggaaaat tcactggctg taagattttc taagtataag atttactgaa
     8101 tgtagctttg gagtttctaa actgaactat aagtaagttt agacggttag aaccgttaaa
     8161 ttctttgaga gtcaaagttg ataaaataac attaaccaat aatttaatta tatgttaatg
     8221 tattttaagc tttggaatta aggtggtaac gcgagctttt cgtccttttt aaagaggatg
     8281 aagagctctt ttttatttct aaagatattt aaaaaattta tttataaaat acaggaggca
     8341 ggacaatgga aaatacgaat ttatcaaaaa cttataaccc aaaagatttt gaggctaggc
     8401 tatataaaaa atggatggat gaagggtatt ttaaatctaa accaaatcca gataaaaaac
     8461 catttacaat aatgatgcca ccaccaaata taacagggca attgcatatg ggacacgctc
     8521 ttgaccatac acttcaagat atattaatca gatggaaacg tatggatgga tatgaagctt
     8581 tttggcttcc aggaactgac catgcttcta tagcaacaga agttaaagtt gttgaaagaa
     8641 taaaaaaaca agaaggcaag acaaagtatg aaataggtag agaagaattt ttaaagagag
     8701 cctgggaatg gaaagatgaa tttggaggaa aaatatctaa tcagcttaaa caactaggtg
     8761 attcttgtga ttgggataaa gaaagattta ctatggatga agggtgtaat gaagcagtta
     8821 tagaattttt tgtaagccta tatgaaaaag gtcacatata cagaggaaat agaataataa
     8881 actggtgccc agattgcaaa actactttat cagatgcaga agttgaacat gaagaacacg
     8941 acggaaactt ttatcatata aagtacccac taaaagatag tgaagacttt ttagaaatag
     9001 ctacaactag acctgagaca atgataggag atactggtat agcagtaaat ccagaagatg
     9061 atagatataa acatttaata ggaaaaacag caatacttcc attagtagga agagaacttc
     9121 caatagttgc agatagttat gttgatttag agtttggaac tggtgcagtt aagatgactc
     9181 cagcacatga ccctaatgat tttgaagtag gattaagaca taatcttgag cagttaaata
     9241 ctatgaatga agatggaact atgaatgaag tttgtggaaa atatgaaggt atggatagat
     9301 ttgagtgtag aaaagctatc gtagcagact taaaagaaca aggttaccta attaagataa
     9361 aagagcataa tcataatgta ggaacttgtt atagatgcca tacagtagtt gagcctagat
     9421 tatcagagca atggtttgtt aagatggaag aacttgcaaa accagctata gatattctta
     9481 aaaaaggaga attagaattt gttccagata aatttgataa aacttattta caatggctag
     9541 aaaatataag agattggtgt atatcaagac aattatggtg gggtcatcaa ataccagctt
     9601 attattgtca agagtgtgga gaaatagtag ttgctagaaa aatgccagat aagtgtccta
     9661 aatgtggaag cactcatttt aaacaagatg aagatgcctt agatacttgg ttcagttctg
     9721 cattatggcc tttctctact ctaggatggc caaataagac tgaagcttta gattattatt
     9781 atccaacaag tgtacttgtt acaggatacg atataatatt cttctgggta gttagaatgg
     9841 catttgcagg tatgttctgt atgaatgaaa agccatttga ccatgttttg gttcatggat
     9901 tagtaagaga ttctcaaggt agaaaaatga gtaaatcttt aggaaatggt atcgacccat
     9961 tggagataat cgagcaatat ggagctgatg ccctaagatt tacattaaca actggaaatt
    10021 ccccaggaaa tgatatgaga ttctatatgg aaagagtaga atttgctaga aactttgcaa
    10081 ataagttgtg gaatgcatca agatttgtat ttatgaatat tgatgaagat ataattaaaa
    10141 acatgactag agaaagtgtt aaagaggact taactctagc tgataaatgg ataatatcaa
    10201 gagcaaataa tattgtaaaa gaagcaacta ataacatgga taagtttgat ttaggaatag
    10261 cacttcaaaa gatatacgat tttacttggt cagaatactg tgactggtat attgaaatgg
    10321 ttaaacctag attatatggg gaagatgcaa atgctaaatc tgcagcatta tatacattaa
    10381 cttatgttct tgaaaagata ttaaaattat tacatccata tatgccattt ataacagagg
    10441 aaatatatac tcatcttcct actgttgaag gatgtataat agtatctgaa tggccaaaat
    10501 ataatgaaga agataacatg gctgaagaag aagatatgat gaacttattg atggaaggta
    10561 taagaagtat aagaaatgtg agagcagaaa tgaatgtacc accttctaag aaagctaaat
    10621 tgataataat cccaagtgaa gaaaaaatag aagctattga gcttggtaaa gactatttta
    10681 taactttagc atctgcttca aatgtagaga tagcaaaaga taaatctaat gttccagaag
    10741 atgcagttgg agtagttatt gatggtgttg aaatatttat acctcttaat gaattagttg
    10801 attttgaaaa agaaattgag agattatcta aagaaaagaa aaagttagaa ggcgaaataa
    10861 agagagttaa tggaaagctt gcaaatcaag gtttcttagc aaaagctcct gaaagtttaa
    10921 ttgaagaaga aaaagcaaaa aaagaaaaat ttgaagagat gattaaatct gttgaggaaa
    10981 gattaacaaa tttagaatct aaaataaaat aaatataaga attagtaaaa acatacaaaa
    11041 agtatatatt ctatactttt tgtatgtttt tctttatata tatacaaata tgatgttata
    11101 attctttaca gatgtgttat atttttattt tatgttgtga atatatgaat tttatgtctt
    11161 aaaatttata agcaataaaa atttgatata ttatgatgtt taatataggg taataaatac
    11221 atattatatt tattttacta tattttttta cataatatat ctagaaaagt ataggaattt
    11281 atcgtaattt aagtttaaat attaaagaga atttctataa gtagtttaat cgacaattta
    11341 aatttataaa gaaatttaaa tttgaaagct agtgagtaca gtttggaaat tgaaataaag
    11401 taaactaaat gatattttag atttggaggt gatattatgt tcagggtagt tatatgtgat
    11461 gacgaaaaaa tacaaagaag catactaaaa gagtttacag aaaaactttt gtctgaaaga
    11521 tgtctaaatt atcaaatact ggaattttcc tgtggagaag atttaatatc caaataccca
    11581 gaaaaaattg atattatatt tttagatatc caaatgaaag atatcaatgg aattgaggtg
    11641 gctaaaaaaa taagaaagtt tgatgaaaaa gtagaaatta tatttacaac ggctttttca
    11701 gagtatgccc cccaaggtta tgaagttcgt gcttatagat atttagtaaa gcctatagag
    11761 tatagtaact ttgcaatggg tgtaaatctt tgtattgata accttcaaag aaagaaggag
    11821 cgttatatag ttttaaatag taaaaaagga tttaatagaa tactgataga ttctatttta
    11881 tatgttgaaa ctgtaaagag agatttagtt gtacatacaa taggtagaga ttataaagct
    11941 aatatgagca tgaaacaagc agaaaaatta ctgtgtgaaa atggcttttt tagatgtcat
    12001 acaagtttta ttgttaattt aagaagaata gaagatctta gggataatac gattactatt
    12061 aatgaacaat ttatacctat aagtaagtat agacttaaag atttaaaagt agcattaaca
    12121 agcttgttat gtgatataat gtattaattt taaaaaggta attgaaaata tcgtaaaaaa
    12181 gatttaatgt catcacaggg ctattgattg aaactgtctt aatatataaa atgttattta
    12241 tattaagaca gttttattat gcaaaatagc agtttataga cttttaatat ggtttaatat
    12301 gtatatgtag agtttccatt ataatagcat gattattaat tagtagcttg attatcaata
    12361 tactattatt gatttttata tgtattatat gtatttttag aatatgatta cctttttaaa
    12421 ataatgacta tttataaaac tctatagata tttgtttcat ttagctatg
//
LOCUS       DAMJWS010000103        12261 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMJWS010000103 DAMJWS010000000
VERSION     DAMJWS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.107"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..2107)
                     /locus_tag="PC399_003241"
     CDS             complement(<1..2107)
                     /locus_tag="PC399_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594071.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGI"
     gene            complement(2097..2849)
                     /locus_tag="PC399_003242"
     CDS             complement(2097..2849)
                     /locus_tag="PC399_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2594072.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            complement(4115..4291)
                     /locus_tag="PC399_003243"
     CDS             complement(4115..4291)
                     /locus_tag="PC399_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594073.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(4395..5003)
                     /locus_tag="PC399_003244"
     CDS             complement(4395..5003)
                     /locus_tag="PC399_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2594074.1"
                     /translation="MGKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            complement(5102..5926)
                     /locus_tag="PC399_003245"
     CDS             complement(5102..5926)
                     /locus_tag="PC399_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2594075.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEINQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(6104..6997)
                     /locus_tag="PC399_003246"
     CDS             complement(6104..6997)
                     /locus_tag="PC399_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2594076.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(7254..7691)
                     /locus_tag="PC399_003247"
     CDS             complement(7254..7691)
                     /locus_tag="PC399_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF2594077.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(7948..8097)
                     /locus_tag="PC399_003248"
     CDS             complement(7948..8097)
                     /locus_tag="PC399_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF2594078.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(8110..8340)
                     /locus_tag="PC399_003249"
     CDS             complement(8110..8340)
                     /locus_tag="PC399_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF2594079.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(9158..9379)
                     /locus_tag="PC399_003250"
     CDS             complement(9158..9379)
                     /locus_tag="PC399_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF2594080.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            9959..10216
                     /locus_tag="PC399_003251"
     CDS             9959..10216
                     /locus_tag="PC399_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2594081.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            10685..11923
                     /locus_tag="PC399_003252"
     CDS             10685..11923
                     /locus_tag="PC399_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2594082.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         4564 a         1447 c         1657 g         4593 t
ORIGIN      
        1 aaataccaat aatttttttg aatttcttat aagttaatag atatataagg tacatataaa
       61 ccccacaaat ccctataaaa cctaagttta tcaaaagagc attgacctca ctattgacga
      121 ttctaccatc attatttaac ataatcataa aattaaacag ataaattaaa aaacttcctg
      181 caataagagg gtatgaaaat actttcttta attgatttct aatacagaac tttatataat
      241 cattgtttgc tcctaatcga tttaaatcca taaagacctg cttgtgattt atagctatag
      301 taacacccct tgtatatgca atgattccta ctgctgtaaa acatatgatt gccacatata
      361 tgaataacat taagaagact gccatgtttt ttatcatatc ttggctttgt aaaattttaa
      421 agttaggata gtatttccaa ttaagataca actgattgtt gtttgggtct agttttaaat
      481 gaccttcacc acttggatat tcttcatcca taaaatattt tcttccagtt gattcataac
      541 gtttttttac gtattcatca taaccactga acatgcccat ttccttagat gttcttttta
      601 taatttcttt ctttaagtca ttggcaaaag cataggtatc tttatctaca ttaaatagta
      661 tagaattatt gtaatttttt atggataaat ctttagtaaa tgatttataa tcctcatcat
      721 taaggacata ggcgttcata cttctatttt gaaaaaaaga accattaaat attatttttc
      781 cagcatatct aaaggggatt tctttttctg tgactgggct tgatataaat ttggtatatt
      841 tataatcttg attttcattt cctttattta caacagcaag atattcacca ttttttatag
      901 ttattggttt gccaattagc ttggaaaaat ctttagaatt aaagaacata tcataggagc
      961 tttttttcat atataccttt tcgatagttc catctttatg atatattgat tctacaccat
     1021 caacaattaa ttctatagaa ggaatttcca tatagttttg aatttttata ttgtgtttat
     1081 ctgctaattg ttcaatatct ttttttgata cctgtttatc tctttgattg taggtaaaag
     1141 tataatctat agggttattt ttaagagtat catagatacc tgtaaccata ttaggaacat
     1201 agaatacagc aaataatgct cctgcaatca aaaatgaaat aacacacatg ttttttactg
     1261 tttgtcttcc cataaatctc atcatgctag ttgatatgat gtttttatag tatttctctg
     1321 gatgttttcc tcttttagca tgtacaacta tgtatgtcat aatcatatat attccaataa
     1381 gagagagtag ataagtaaag ctccatatag atggtagttt ataatttaat ttgtttatag
     1441 ttagtgaagg tacgagatat cctagaaaaa taccgagtat aataaaaatt atacctaata
     1501 taccatacca gtcctttaca tcctgtattg gttcagatat tctctggtca tttaatatat
     1561 caattatatt tgtacgatta ataaatttta cagaaagtat aaatatgcat aatgtcacaa
     1621 atacactaaa tataaatcca tatatgaatc cttgagttcc aaaatgatat gtcatttctc
     1681 tggtatcaat aatcactaat tgaaatattt tccatatacc aaaagataca ggaatactta
     1741 atataagacc taaaaaacag catactattg aaattaacaa tatctctgaa aatagaagtt
     1801 tttttaattt cttcttatct gttcctagag ccatgaaaat accaatttcc ttgcttttaa
     1861 attttaaaaa gagggaagaa gcgtatgtag taaagacagc gcatcctaat atagcaactg
     1921 agaatatcat tgtagcctgt tttctgctat ctccaccagt tggaagtatg ttttgtattg
     1981 ttgggctaaa ataaattaga gcatatgaag ttactaatac cacagaaagt attatacaaa
     2041 agaataaagt tgtatagttc ttccaattat aggctcgaag tttactgtat aatttattaa
     2101 ttgtcatctt gagcgcctcc catttctttt aaaacttcta gtaattcatc taggaattta
     2161 tgtcttgaat ttgattttac aacttcttta taaattattc catctttaag cacaataact
     2221 cggtcacaga agcttgctgc aaagctatcg tgtgtaacca tgagtatagt agcatctaat
     2281 tcttgctttg cttttaaaaa tgattcaatg actgtacgac aagacttact atcaaggtta
     2341 ccagttggtt catctgctaa tattaaatct gggttgttca tgagtgctct tgctactgct
     2401 gtacgctgtt tttctccacc agatatttcg gcagggtatt tttttctgat atgttcaata
     2461 ccaaatagct cacatagatt aattgcacgg ttttccattt gtaagacttg actacctttg
     2521 ataatttgtg gaacacatat attttcaaag acactaagac catcaaatag catgaagtct
     2581 tggaatacaa atcctagttg ttgatttcta acttctgaaa gttccttttc ttttaaattt
     2641 cttatattat tatttccaag aattatttcc ccattatcaa ttgggataaa acatgaaata
     2701 caatttaaaa gagtagtttt tccacttcct gatgaaccca tgattgcgac aaattctcct
     2761 ttgttgattt caaaagaaat attttttaat acaggatatg ttttactgcc tgttttatat
     2821 gatttacata attggtttac tttaagcata tatatcatcc tcactttcat ttatcttgta
     2881 agtcaatgat agcatttgaa aggggaggag ggatatgagc tatattgtaa tagttggatg
     2941 tttttttgta atgtttgaaa atatattgtt tggatagtgt gagaggttat tattatagtt
     3001 aaattgatta tattattgga ggatttagag ggttattttt aggtattaat tgttgtgcta
     3061 aagtaatatg atgtggaaca ttgaaggtgt agttagaaaa gaagtatata gtaaaagtaa
     3121 aaaggttata gaataacgaa tgttgtattg aaaaaaatga tgtaaataat gtatagtagg
     3181 gttaaaaaga aaaaaagaat tatagagcag taagattatt taatagaatg tattgtaaat
     3241 aatttgaaag gatttagggg ctattaacac aatgtaattg agaaaatata ttggagtttt
     3301 ggaaataaat aggttgttag aaacaattga aatgtataat aaaaaggtta aaaagtatta
     3361 ttagtatagg tatgatagat aattttagaa agtggaagta ttttagacta tgctgaaaag
     3421 tgattgatat gattttgaga tattaaaata ttactatatt atagtaaaga agttaaaata
     3481 ataaaatgtt gtaataataa aggtttgtag tataataaag aactgtaata aataagactt
     3541 tgcaatgtaa tagaaatgct gtaataataa aagtctgtag tataataaag aactgtaata
     3601 aataagactt tgcaatgtaa tagaaatgct gtaataataa aggtctgtag tatagcaaag
     3661 aactgtaata aataagagat tgtaataata aaagtctgta gtgtaataaa gaactgtaat
     3721 aaataagact ttacaatgta atagaaatgc tgtaataata aaggtctgta gtataataaa
     3781 gaactgtaat aaataagact ttacaatata atagaaatgt tgcaataaca aaacaccata
     3841 ataaataaga gtttgcaata taatagaaac actgcaataa ataagagatt tcaatataat
     3901 aaaagtctgt aatataacaa aatcctataa taaataagat tttacaatat aataaaaatc
     3961 ctataataaa taggactctg taatataata aaatgctgta acaacaaaac actataataa
     4021 ataagaccct gtaatataat aaaaatacta taatatagta aaaatcatgc aataataaaa
     4081 atctataata ataaacctca gcataacaaa gacattaatt tcttactaaa aaggccacca
     4141 caattaacag tgaaaaagca attgaaagta ttgaaaaagt aaaatgagaa ttagagtcat
     4201 tattacgatt attaagtttt atccataaac cacatataat tgtacacaac aataaaattg
     4261 ctgatattac tcctataaat gtaaaaaaca ttaaaattcc tcccccttat ttaatgcaag
     4321 tatttttagt caatgagaat attttcaaac gttaccataa aataaaaaaa tgtacataaa
     4381 aaattaatat agtattattt tgtaatatct accaataata aatctaattg aaaatctatt
     4441 aaaccatttc tctgttcaat attaagaata tcaataccac tatacttgaa aaaagcatta
     4501 gaacgaagaa aaagcaactg aataaatgaa gttatttgat acgcaatagt tctgtattca
     4561 gcttcagttt ttttgttatt ctgattttca tagtgagcct taattaaagg tagtatctca
     4621 aatggataaa caatttcttc ctgtatcaat atatagggta cagaaatctt tataaattga
     4681 ctatgttcac ctataaaatc aaaaaatgca attagtattt cccttaattg ctgtttaggt
     4741 gctaaatttt tagtatcatg tgtagcctta tattcatcca tatgacgctt taaaaatttt
     4801 ttaatagaaa tattaattaa ctcctcttta gatttaaaat aataattaat catagctaaa
     4861 ttgacatttg ctttagctac aatttctcta gccgtaatgc ttgaaacatc tctatgtgaa
     4921 gatagtaact ggattgtggc attaattaat tgttcctttg ttgaatctgc tttcataatt
     4981 tttcccctta aaagtttccc cataaaaaaa ttaaacatgt ttaacacacg tttagatact
     5041 atcatgaaaa ttttgggatg tcaatgggtg attagtactt aagtatttaa gtactaatct
     5101 attagacttt tataaggggg atttgaattt ctgtaagata attttcagga tttatttcat
     5161 tatgttcacc tttatgtccg atttgtctat ttatacctga tatttgataa tcactatttt
     5221 tatctaacca ataacagaac gattcatatg caccagctat attttcatat ggaccataaa
     5281 ccatagtaca tgccatcata tcaatttctt ctgtctcacg atatataaat ccagatttgt
     5341 tctttccaat cttctttaca accattccaa cttcaatatc tacaccatta tctttgactt
     5401 cttcatcatg gtaaaaagct atgttattgt tggattgacg tgatacttct atatgttctt
     5461 ctttgataaa tttagatagc ttttcccaga gtataccttc agattggtag tctggaatta
     5521 tctctctaag tgatatgatt ttataacttg gaatcttttt aaagacaaca ttgtaatgaa
     5581 tttcatcttt atcacagttt atagcttcta taaatttatc aattttattt attctttgtt
     5641 gttcctgatt tatttccttt tgtatttcat tcttttttct attcaattct ttaattacaa
     5701 attcatcatt ccaattatgt acgatatttg ctatttctgc aacgctaaat tttgaatctc
     5761 tcaataatac aattctttgt agtattgaaa tctgttcaac tgagtataat ctatgacctg
     5821 tatatttgtc tatttctgca ggttttaaaa taccaagttc atcataataa cgcaacatac
     5881 gaatagaaat ttgtgttagt tttgaaacct ctcctatttt aaacatgaaa atatgacctc
     5941 cttttcactt ataatcagta tatattttgt ttttatatac ttcaagcgtt tgtggaaata
     6001 caattatttt ccagatgttg caataaatta tagagagtaa attatattta agtagtaatc
     6061 gtatcaaatc atagattatc ttaatttata attcatatta aaattaccag tctaactcaa
     6121 attcaaaatc atccgacttt atctgttcaa tttcagaatt taccttctgt ttaaatttat
     6181 tagaagaaat catatataca agaaatatta aaccaaatat tatttgtaca tattgagcta
     6241 ttacacataa tttaataggg tagtgtagaa gtaattgaat gttgaaaaaa tagtacatca
     6301 tatgaactaa cccagctgat aaaatgcttc tacttgtatt gatatataca aaattaatta
     6361 taaagtttaa aagtacaatg tttggtataa ataaaaaagc atcaaatagg gagtattgtg
     6421 ccaaattata tggtatttga ttaggtgtaa aatacgaccc tagataccag attccacaaa
     6481 caagccctaa aataattgaa gagcctataa atccaaatct aactaataat ttgtcaatgg
     6541 catatcctct ccatccagct tcttgactaa gtatactcaa tataaaactt gctaatagca
     6601 tataagctat tttagaaggt ctaaacactg ctatgtgtat ccaatctata tcagaagttt
     6661 ttacattaaa gtaatttccg attattaacg aagtaataaa agttaaagca aaaaataata
     6721 ttgttaatag tatccatttt attctaattt ctttaaagct taggtatcta taaataaaat
     6781 cttgttttgc actttgtgga tagattgtag aaataaaaaa tagacctaca atagctggtg
     6841 atgtatatgc aagataaaaa atgatttctc caagtattgt atttgtaatt ccgagcaacg
     6901 caggtgtaag actgaatatc cagttcaata ctagcgtaaa tgtaaagaaa tagattaagt
     6961 gtttgtttgt attaaagggg gtgttatata gttgcatttt ttcactatcc ttccgtttta
     7021 taatgaagtt ttaattttta ttaattgttt atttattttt atatgtatta agttatcgct
     7081 tgatgttagt tattgcctat aataatatcc tattttttat attttgatta attattttta
     7141 cactaaatat aattaattag tatttaatat tgaatatatt atattattat taattattaa
     7201 ataggtcaat aaatttgcaa ttagtgatat taataaatta ctttagattt ttattaaatg
     7261 tttagctttt ctttaatatt ttttatataa cgacgattta taataacttt ttcgttgttt
     7321 tttaaagtag cttgcatgcg attatgtgat attactttta tactttttat gtagtccata
     7381 tttaaaatca tggatttaga tattcgtgag aaggatgtat tagctaataa ttttgataat
     7441 ttatatattt ttaaatcaat ttcaaataca tcatcttcat aataagcaaa tgttttattt
     7501 ccaactgtct caaagtataa aatatcacag acattgagtt tataaatctc tcctgatttt
     7561 ttacctttta tatcaagtaa cggtgtactc aataatgaaa ttattttttc aatgttatta
     7621 tctagagcag aacattttat ggttatttca atatcatcac aattaatacc ttgttcgaca
     7681 tgtattttca ttaatcatca cctcatttta ttactgtcat tagaatagca ttatatctta
     7741 ttgagtccaa ctagatgcgt tctaaatttc aaattaagca tagtgaaatt caaaaataaa
     7801 acagtgaaat tcaaatttga ggtggtaaaa ttcaaaaaaa tactatggat atttggataa
     7861 attatccaaa tatcctagta tttcacatag aatatgtcta attattttac aaatagacct
     7921 attttatgac ttacatatat tgtcaaatta cttcttaaat atcaaaatat ttttggataa
     7981 tgtatcaata ctgtttgata aagtatttaa tttaccctca attctaacca ataagtacat
     8041 ggatagagct ataggaaaac caacattggt tattaacagt tgtaaactat catccataaa
     8101 attagccccc tattctataa ctaagtcata aggagtggta tttgtttcca caaccttggc
     8161 ttcaacaaca gaagctaggt caaatcctcc aaccgaaaaa atatttttag atactatcaa
     8221 atccatgaca gattttatat tttcttcagt taaatcatcc tttggatttt ctatagaaaa
     8281 agaaacttgt ttatccttta catttttaaa gctcatgact aattttttct taatttccat
     8341 ataattacct ccgatataat aatttatatt ttaattgttt gcttgttaat attggtattt
     8401 taattgtcaa tattaatgtt ttgcttgtta gtattaatat ttcaattgtc aatattaatg
     8461 ttttgcttgt tagtattaat atttcaattg tcaatattag tgtcttgctt gttagtatta
     8521 atatttcaat tgtcaatatt aatgttttgc ttgttagtat cagtgtttca attgtcaata
     8581 ttagtgtttt gcttgttagt attaatattt caattgtcaa tattagtgtc ttgcttgtta
     8641 gtattagtat ttcaattgtc aatattagtg ttttgcttgt tagtattagt atttcaattg
     8701 tcaatattag tgttttgctt gttagtatta gtatttcaat tgtcaatatt aatgttttgc
     8761 ttgttagtat tagtatttca attgtcaata ttagtgtttt gcttgttagt attaatattt
     8821 caattgtcaa tattagtgtt ttgcttgtta gtatcagtgt ttcaattgtc aatattagta
     8881 ttttaattgt tgactctagt tattgctgat atagaaaata tttaatttat ttcaattaat
     8941 tagattaaag tagggtagca tctaaaatag ctaacatgtt taagttgaaa tacgtttaat
     9001 atattaattt ttcaaagcat taatatatta aagttataat caaaattgaa gttataatct
     9061 gccaaatcta tagcgtatta agtgcgttat ataacttaat cttttatata aagagttaat
     9121 ttttttaatt tgtaaagcag agtacgttta aaaggtacta tttagataat gatgtactat
     9181 ctaattttaa aatatctaca atagggttct cttgaagact tgctattgct tctgcaacag
     9241 cataaacatc ttcatttaat gcctctgctt ttacatttgc aaatgattta cttttaacaa
     9301 ctgccttacc cttatcatca aaaccagcat ccaattttag ttttatacta gataaatttt
     9361 ttgttgaaac aacttccata aaattacctc ctaaaaattt aatttcaaag atttgtcgac
     9421 tttctttgtt agctataata ctatcatcta agataatatc ggagttaaac agtaatttct
     9481 aaagattttt tgtgtaaatt tttataggaa aaattttact tttttgctga tgaaaaagtt
     9541 tacaagttat ttgtgaagat atgttcttag tgttttgaaa tagtatttgt atatattcat
     9601 gcttagttga gatagttttg tatggatttt catatgtgta gagttaaatg ttaggtacta
     9661 aagtttatgt taaattaaat gttaaagctt atgttaaaaa ttttattaaa tttaatctgt
     9721 aaataaaaaa gttaaaagtc caatattaac attttacttt taaaccatta aaatctttat
     9781 taaagctaac aaagttataa gttatgagaa attttattaa agttaataca aaaattaatt
     9841 ttaaagctaa gaaatttttt attagattca ataaaactaa taaatatagt ttagtcaata
     9901 atgtattaaa taaaaacttt gttaagtcta taaaaatatc ttaaaggagg tgtaacttat
     9961 gaagataaaa gtggaggata taccagttca atttcatgca atggtagaaa taattggaat
    10021 tgataagttt atagaggttg ccaagttgta tggaggaaca aatacttata tacctacgta
    10081 taaggggctt tttagatatg ctagaaatag ggatatagta aaacaattta atggagtcaa
    10141 ccacaatgaa ttggctatta gatataatat gtgtgtaagc aatattaagc gaatactcaa
    10201 tgaaaataat gtttaaatat aataaaataa tgtttaaaca taatgaaaat agccttgtta
    10261 aacgtaagag taaagacaaa gaagatgttt tataaagttt ttttagacaa attaactaaa
    10321 tttactactt attaatttaa atacattgta tattctataa aaaaatttgt taaatactgg
    10381 agatatattg agaatacaaa gaaaatggtc gaaaatattt gttggctaaa aaatagataa
    10441 aatgatacaa ttgcttgtat aaaggggaaa aataaataaa taaactatat aaataaagaa
    10501 atagcaatta atatgacata atatatttat aaaattaaga atataaatag atattttaag
    10561 gtattagttg ttaaattagg aaatattaaa aaaaatatat atttatattt aaactatttt
    10621 gttatttata taaaaatact tatctagtta ctagcaatta ttagtagaaa tgggggaaat
    10681 agagatgaat aagggatttt acaaattaaa ttcagtggta ttgaactttg atattaagaa
    10741 tgatgttaac aagataagat ttaatcatta tataatgaga aaagaaaaca tacaaagact
    10801 tcaaggaact ctgccaaacg gacaatttta tatgacagtg agaagagctg ctacagattt
    10861 acaactttct atttctacta taagtaggct tgtaaaagag tttttggatt tagggataat
    10921 aagattagtg tctaagggtg taaaaggtaa tcgttctgta tatagttacg taagtataga
    10981 gtttaatgga agtttatttg atgatagcaa atatatggaa cttgactgtg tatgtaatga
    11041 tgaatttatt gataatagtg gcatagagtt tgattgtgat agcaaagata attcttttaa
    11101 tggagatata aatgtagaaa ctgtgcattg cattaatgag tatttgtctg atgatgatgt
    11161 agagcataat tataacaatt tccataataa agatactata aaggataaag ccatttctaa
    11221 tattagcact atcaaaaaat atattgatga atcttttgat aaaaataaat cagaaaattc
    11281 taacttacaa caggagtcaa taaatttcaa ctttaatgat acatgcaatg aaatagataa
    11341 aagaacgaat aatagtatag aaaataatca aaataaaagg cctatgagat tgagtgacat
    11401 cagtctttta agtgataaag gtatgacaaa taaaaagaat tactttaatc ataatcatgg
    11461 tatacaaaaa gggactaaat ctgtaacaaa gaaaaaagaa tccttaaata aatttaataa
    11521 aaaaaatata cagcaaagca ttataaatat gctaaataaa aaatcaggta agaattttaa
    11581 aatcgattct ccaataagca taaaacttat caatgaaagg ctaaaggaag gctatgtctt
    11641 agaagatttc tacaaagtta ttgaggtaaa agtggctaag tggaaaaata ccataatgga
    11701 aatgtatata agaccagaaa ctttgtttag ctataagttt gaatcctatg taaatgaaaa
    11761 catatctatg aaaagtcaaa gtaatgttca cccatgtaaa gaaggcaata gtaatgatag
    11821 cagtaattat atacattctg attatacaac atatgatggc agtgtgcgtg agagaaaaaa
    11881 atatgagaat gtaagatact ggaatcctat atgtgaaatt taattagttt aagaatgtat
    11941 ttatgataaa taagatatta atagagaata gtgagctata gcaaatgata tatcaatagt
    12001 tggtagtaag ttatattaag caagatatta ctatcaggta gtgaattata ttgaacgata
    12061 tatcaataat tggtagtaaa ttatattaag taagatatta ctatcaggta gtgaattata
    12121 ttgaacgata tattaataat tggcagtaaa ttatattaaa taagatatta atatcgagta
    12181 atgaattata ttgaacgata tatcaataat tggcagtaag ttatatcaaa taagatatta
    12241 atatcgagta atgaattata t
//
LOCUS       DAMJWS010000104        12215 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMJWS010000104 DAMJWS010000000
VERSION     DAMJWS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.108"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            219..1007
                     /locus_tag="PC399_003253"
     CDS             219..1007
                     /locus_tag="PC399_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2594083.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            1478..5194
                     /locus_tag="PC399_003254"
     CDS             1478..5194
                     /locus_tag="PC399_003254"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF2594084.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIVKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            5236..8721
                     /gene="rpoC"
                     /locus_tag="PC399_003255"
     CDS             5236..8721
                     /gene="rpoC"
                     /locus_tag="PC399_003255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF2594085.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            9010..9432
                     /gene="rpsL"
                     /locus_tag="PC399_003256"
     CDS             9010..9432
                     /gene="rpsL"
                     /locus_tag="PC399_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF2594086.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            9556..10026
                     /gene="rpsG"
                     /locus_tag="PC399_003257"
     CDS             9556..10026
                     /gene="rpsG"
                     /locus_tag="PC399_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF2594087.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            10075..12141
                     /gene="fusA"
                     /locus_tag="PC399_003258"
     CDS             10075..12141
                     /gene="fusA"
                     /locus_tag="PC399_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2594088.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4737 a         1536 c         2503 g         3439 t
ORIGIN      
        1 aattaaaata taaacaattg ttgtaatgtc tatatataaa gtatataatt aaactaaaat
       61 gtaaaatttt acattttagt ttaaaaaaat acacgaatgt tttataaata aaatgtaata
      121 aataattaat taacaaatat aaattttaaa ataagttaga tatttagaaa aatcgaaatt
      181 tgtaggattt aaaggacata ttcaaggagg agttaataat ggaaaaatta caaggaaaaa
      241 ttgcagtagt tactgcagca acaaaaggta ttggattagc atcagcagag atattagcaa
      301 aaaatggagc aactgtgtac ttagcagctc gttcagaaga attagctaat gaagttataa
      361 ataaaataag tgcagaaggt ggttgtgcta agtttgttta ctttaatgct cgtgaagaag
      421 agacctttac ctcaatgata gaagaggtag ttaaaaaaga aggtaagata gatatacttg
      481 taaacaattt tggttcaaca aacccttctc ttgacaaaga ccttgtaact ggggatacag
      541 ataatttctt tgacacagta aatactaatt taaaaagtgt gtatttacca tgtaaagcag
      601 caattcctca tatgataaag aatggaaaag gaagtatagt aaatatatca agtataggtt
      661 cagtattacc tgatttatct agaatagctt actgtgtatc aaaagcagca atcaactcat
      721 taactcaaaa tatagctaca caatatgcta aagacaatgt tagatgtaat gctgtacttc
      781 cagggcttat tgcaactaaa gcagcattag acaatatgtc tccagagttc ataaaagaat
      841 ttttaaagca tgttccttta aatcgtatag gggaaccaga tgatatagca aaagctgttc
      901 tattctatgc tagtgatgac tcatcattta taacagggga tttacttgaa gttgcaggag
      961 gatttggttt acctactcca caatttgcag ataatatatt aggataatta aattactaat
     1021 atgcaatgaa agcttatgca ttagttatag tagaaatgat atttttataa aggccataga
     1081 tataatttat attggaaagg tttgaggttc aaaattccaa gttaaattta tttatggtct
     1141 ttattttgta tattttaata aattgtatat atttgtataa atgtaattat ttacaatcat
     1201 tttaatatgg aattataatt aaataggggg gatggtactt gtataaaatt ttttatagaa
     1261 aattagacct ataaatgaaa aatatggatt ttatattgga aaattgtaca taatacataa
     1321 tatatgctta aaataattta caaaaaatca aaaataatgc atagtttaca tataagataa
     1381 aaaatatatt gaaaaatact tgacattatg ctaaaattat tagatgcgtt agaatgaact
     1441 attgttttat ttttaattat tgagaggtga aaagtgaatg ccacatcctg tcacgatagg
     1501 taaaagaact agaatgagct tttctaaaat taaggaaata gctgatgtgc caaatcttat
     1561 agaaattcaa gtagattcct atgagtggtt tttaaaagaa ggtttaaaag aagtgtttga
     1621 tgatatttca ccaatagaag attatactgg taatcttata ttggaatttg tcgactattc
     1681 tctagatgat aaaccaaagt acgatataga agagtgtaaa gagagagatg ctacatattg
     1741 tgcacctcta aaggttaagg taagacttat aaataaagaa acaggtgaga taaaagaaca
     1801 agaagtgttt atgggtgact tccctttaat gacagaaaga ggaactttcg ttataaatgg
     1861 agcagaaaga gttatagtaa gtcaattagt tagatctcct ggtgtttact atgcggaaga
     1921 aagagataaa acaggtaaga gattgatttc atctactgta attcctaata gaggagcatg
     1981 gttagaatat gaaacagatt ctaatgatgt aatatctgta agagttgata gaacaagaaa
     2041 acaaccagtt acagttttac ttagagcttt aggaatagga acagatgcag agataattga
     2101 ccttttagga gaagatgaga gattatctgc tacattagaa aaagataata ctaagacagt
     2161 agaagaaggt cttgtagaga tatacaaaaa attaagacca ggtgaacctc ctactgtaga
     2221 aagtgcatca tctttattaa atgctttatt ctttgaccca aaaagatatg acctagcaaa
     2281 agttggtaga tataaattca ataagaaact tgctttatgt tacagaataa tgaataaaat
     2341 ttcagcagaa gacataataa atccagaaac tggagaagta tttgtaaaag ctggagaaaa
     2401 aataagctat gatttggcta aagccataca aaatgcaggt ataaatgtag taaatctact
     2461 aatggatgat gataaaaaag ttagagttat aggaaataat tttgtggata ttaagtctca
     2521 cattgatttt gacatagatg atttaaacat aaaagaaaaa gtacattatc caactttaaa
     2581 agaaatttta gatggatata gtgatgaaga agaaataaaa gaggctataa aatcaagaat
     2641 taaagaactt ataccaaaac atatattatt agatgatata atagcttcaa taagttatga
     2701 gttcaatata ttctataata taggaaatat tgatgatata gatcacctag gaaacagaag
     2761 aataagatct gtaggtgaat tgttacaaaa tcaagttaga attggtcttt caagaatgga
     2821 aagagttata aaagaaagaa tgacggttca agatatggaa gctataacgc ctcaagcgtt
     2881 agttaacata agaccagttt cagctgcaat aaaagaattt tttggtagtt ctcagttatc
     2941 tcagttcatg gatcaaacaa atcctctatc tgagttaacg cataagagaa gattatcagc
     3001 ccttgggcct ggaggtcttt caagagaaag agctggattc gaagtgcgtg acgttcacca
     3061 ttcacactat ggtagaatgt gtccaataga gactccagag ggaccaaata tcggtcttat
     3121 aaactctcta ggaacttatg caaaaataaa tgaatttgga tttatagaat caccatatag
     3181 aaaatttgat aaagaaacat caacagttac tgatgaaata cattatttaa ctgctgacga
     3241 agaagattta ttcgtaagag ctcaggcaaa tgagccatta acagaagatg gtaaattcgt
     3301 aaaccataga gttgtttgta gaactgtaaa tggtgctgtt gaaatggttc ctgaaagtag
     3361 agtagattac atggatatat ctcctaagca ggttgtatct gttgccactg ctatgatacc
     3421 tttcttagaa aatgatgatg ccaaccgtgc ccttatggga gcgaacatgc agcgtcaggc
     3481 agtgcctcta gttagaagag aagcaccaat tataggaact ggtatagaat acagagcagc
     3541 aaaagactct ggagcagttg ttgttgctag aaattctggt atagcagaaa gagtaacagc
     3601 agatgaaata attataaaaa gagaagatgg aaacagagat agatataatc tacttaagtt
     3661 taaacgttca aactcaggta cttgtataaa tcaaacacct ataataaata agggtgacca
     3721 gatagtaaaa ggtgatgtta tagcagatgg tccagcaact gatttaggag aagttgcact
     3781 tggaagaaac tgtcttatag cattcatgac ttgggaaggt tataactacg aggatgctat
     3841 attaataaat gaaagattag ttaaagaaga tagattatca acaattcata tagaagaata
     3901 tgaatgtgaa gctagagata caaaactagg gccagaggaa ataactagag atatacctaa
     3961 tgttggagat agtgcaatta agaacttaga tgacagaggt ataataagaa taggtgcaga
     4021 agtagactca ggtgatatat tagttggtaa agtaactcca aaaggagaaa ctgaacttac
     4081 tgctgaggaa agattacttc gtgcaatatt tggagaaaag gctagagaag ttagagatac
     4141 ttcacttaaa gtgcctcatg gtgaatctgg tataatagtt gatgtaaaag tatttactag
     4201 agaaaatgga gatgatttat ctccaggagt aaatgaatta gtaagatgct atatagctaa
     4261 aaagagaaaa ataaaagttg gagataaaat ggctggtcgt catggtaata aaggggttat
     4321 ctcaagagta ttacctgaag aagatatgcc attcatggaa aatggaacac cactagacat
     4381 aatacttaac ccacaaggta taccatcacg tatgaacatt ggtcaggttt tagaagttca
     4441 tttaggactt gctgctaaga cattaggatg gtatgtagca acatcagtat tcgatggtgc
     4501 taatgagtat gacataatgg atgctcttga agaagctggg tatcctagag atggtaaatt
     4561 aactttatat gatggtagaa caggtgagtc ttttgacaac agaataactg ttggatatat
     4621 gtactacttg aaactacatc acttagtaga tgaaaaacta catgcaagaa gtactggacc
     4681 atactcatta gttactcaac agccactagg tggtaaagca caatttggtg gacaaagatt
     4741 tggagagatg gaggtttggg ctctggaagc atatggagct gctcacatac ttcaagaaat
     4801 acttactgtt aagtctgatg acgttgtagg tcgtgttaga acatatgaag ctatagttaa
     4861 aggtgaaaat atacctgaac caggaatccc agaatcattt aaggttctta taaaagaact
     4921 tcaaagttta tgcttagatg taaaagtatt gacagatgaa gaccaagaaa tagaagtaag
     4981 agaatctgtg gatgaagatg atacaatagg tgaatttgag ctagatgttg taaatcatat
     5041 gggagaagtt gaagaaagta atataataga agaaattgaa gatgattttg cagaaaatgc
     5101 agaagatgag gatatagaga atttagaaga attcactgaa gatgatttat ttgaagaaga
     5161 aatagatttt gatagtgatg actttgatat gtaaatttaa ttaatgtaaa tctcaaatag
     5221 aagggagaga actccttgtt tgaattaaac aatttcgagt cgataaaaat agcattggct
     5281 tctccagaaa aaataagaca atggtctagg ggagaagtta aaaagccaga aactataaat
     5341 taccgtactt taaaaccaga aaaagatggt cttttctgtg aaagaatatt tggaccacaa
     5401 aaagactggg agtgccactg tggtaaatat agaagagtta gatataaagg tgtagtttgt
     5461 gatagatgtg gagtagaagt aactaaatca aaagtaagaa gagagagaat gggacatata
     5521 gagctagcgg ctcctatgtc tcacatctgg tacttcaaag gtataccaag tagaatggga
     5581 cttttacttg atatgtcacc aagatcgtta gaaaaaatat tatactttgc ctcatatgtg
     5641 gtagttgatc caggagagac tggattaaat gaaaaacaat tgcttacaga aaaagaatac
     5701 agaactgctc ttgaaaagta tggatatact tttactgtag gaatgggtgc tgaagctgta
     5761 aagacattac tacaaaatat agatttagag caacaaagta aagacctaag agcggagtta
     5821 aaagatagta cagggcaaaa gaaagttaga acaataagaa gattagaagt tgtagaggca
     5881 tttaaaaagt ctggaaataa accagaatgg atgattttag atgcaatacc agtaatacca
     5941 ccagatttaa gaccaatggt acaacttgat ggtggaagat ttgcgacttc agacctaaat
     6001 gatttatata gaagagttat aaatagaaac aatagactta aaagattatt agagcttgga
     6061 gctccagata taattgtaag aaatgaaaaa agaatgcttc aagaagctgt tgatgcattg
     6121 atagataatg gtagaagagg tagacctgta acaggacctg gaaatagacc acttaaatct
     6181 ttatcagata tgttaaaagg taagcaaggt cgtttccgtc aaaacttact tggtaagcgt
     6241 gttgactact caggacgttc tgttatagtt gttggaccag aacttaaatt ttatcaatgt
     6301 ggacttccaa agaagatggc attagaatta ttcaagccat ttgttatgga taaattagtt
     6361 aaagaaggtt atgcacataa tataaaaagt gcgaaatcta tagtagaaaa agttaagcca
     6421 gaagtttggg atgttttaga agatgttata aaaagtcatc cagttcttct taaccgtgca
     6481 ccgactctac atagattagg tatacaagca tttgaaccaa tcttagttga aggtaaggct
     6541 ataaaactac atcctcttgt atgtacagct tataatgcag actttgatgg tgaccaaatg
     6601 gcagttcatg tacctttatc agtagaagca caagcagaag caagattctt aatgctttct
     6661 gtaaataata tacttgctcc taaagatggt tcacctataa ctactccatc tcaggatatg
     6721 gttttaggtt gctattatct aacaatagaa gcccaagatg gagctaaagg aacaggtatg
     6781 gtatttaaag actttaatga actattactt gcttattaca ataaatcagt tcatctacat
     6841 gcattagtga aacttaaggt aacacttgaa gatggaagaa gttcattggt tgaaagtact
     6901 gttggtagat ttatatttaa tgaaaatata cctcaagact taggttttgt agatagaaaa
     6961 gaaaatccat ttgcacttga agttgatttc ttagcagata aaaaatctct tggtaaaata
     7021 atagataaat gctttagaaa acacggaaat acagaaacag ctgaattact agattatata
     7081 aaagctctag gatttaaata ttctacatta ggtggcataa cagttgctgt tgatgatatg
     7141 agtgtcccag aagaaaagaa agtatttata gctgaggcag aagctaaagt tgataagtat
     7201 gaaaaagcat acagaagagg tctaatctct gatgaagaaa gatatgaaaa agttatagag
     7261 acatggacag aaacaactga taaagttact gatgctctta tgggtggact agatagatta
     7321 aataatatat atataatggc acattcagga gccagaggtt ctaaaaacca aattagacag
     7381 ctggcaggta tgcgtggtct tatggcaaat gcatctggta aaacagttga gataccagtt
     7441 aaatctaatt tccgtgaagg tttatcagta ctagaatact ttacatcttc acatggtgcc
     7501 agaaaaggtc ttgccgatac agctatacgt acagctgaat ctggatattt aacaagaaga
     7561 cttgttgatg taagtcaaga tgttattgta agggaaatag attgtggaac agaagatact
     7621 acagagattt atgctataaa agaaggaaat gaagttatag aagagatata tgatagaatt
     7681 gtaggaagat atactataga ccctatatta aatcctgaaa ctggagaagt tatagttgag
     7741 gctgattcaa tgatacaaga agatgaagca gaaactatag tagctttagg aattgaaaaa
     7801 attaggataa gaacagttct taactgtaaa actaatcatg gagtttgttc taagtgttat
     7861 ggtagaaact tggcaacagg aaaagaagtt aatataggtg aagcagttgg tataatagca
     7921 gctcaatcta tcggtgagcc gggtactcag cttacaatgc gtacattcca tactggagga
     7981 gttgcaggag ctgatataac tcaaggtcta ccaagggttg aagaattatt tgaagctaga
     8041 aaaccaaaag gattagctgt aataactgaa gtatctggta gagttgaaat tgatgaaaca
     8101 ggaaagagaa aagaagtaaa tgtaatacca gaagaaggcg agactcaaac atatgtaatt
     8161 ccatatggtt ctagattaaa agttaagcaa ggtcaaatgc tagaggctgg agaccctcta
     8221 acacaaggat ttataaatcc tcatgatata gtaagagtaa atggtgttaa gggagttcaa
     8281 gaatatatag ttaaagaagt tcaaagagtg tatagacttc aaggggttga tgttaatgat
     8341 aagcatatag aagttatagt aagacaaatg ctatctaaag taaaagttga agacccagga
     8401 gatacagatt tattgccagg tggatatgaa gatgtattaa cattcaatga atgtaataaa
     8461 gatgctatag ataaaggtct aagaccagca gttgctaaaa gagttttact tggtataact
     8521 aaagcatctc ttgcaactga ttcattctta tcagcagctt ctttccaaga aacaacaaga
     8581 gtattaacag aggcagcaat aaaaggtaaa gaagaccact taataggact taaagaaaat
     8641 gttatattag gtaagttaat accagcagga acaggaatga agaaatatag aaatatagct
     8701 gttgaaaaaa ttgaagatta atagcatata gagtaaagaa atcttgacaa ccattatttg
     8761 tggtgttaaa atagtaaagt gtgttgttta aaagtacact aagggcttga acaagttctt
     8821 agtgactttt agatggcaat aaatgagaac taaaaagcat caagctagaa aagtgctaat
     8881 atcacatatt tatgtgttat agacattaaa ttgtaagtaa cgaatgttac aatcagtcag
     8941 tatgaatatt catattgatg tagttggcgg tcactgataa gatcgaaaat ttggaaggag
     9001 gtgcagatga tgccaacaat taaccaatta gttcgtaaaa gtagaaaagc gctagagaag
     9061 aaatctactg ctccagcatt acaaaaaggt tacaactctt taaataagaa agtaactgac
     9121 gctagtgccc cacaaaaaag aggggtttgt acttcagtaa aaacagttac tcctaagaag
     9181 cctaactcag ctttaagaaa agttgccaga gttagattaa ctaatggtat agaagtttct
     9241 gcttatatac caggagaagg acacaactta caagagcata gtgttgttct tataagagga
     9301 ggaagagtaa aagaccttcc aggggttaga taccatatac taagaggtac tctagataca
     9361 gcaggtgttg ataagagaag acaatcaaga tctaaatacg gtgcgaagag acctaaagaa
     9421 gctaaaaagt aaaaacaatt aatgattgat tatagttcgg tgctttaaat atatatatat
     9481 ttacggcatc acggcattaa atgatttatg tcgagtacct atgatttaag ttaaataatt
     9541 aaggagggaa gcgaaatgcc aagaaaaggt aatattccaa aaagagaagt tttaccagat
     9601 cctatgtatg ggagtaaggt agttactaaa ttaataaata atctaatggt agacggaaaa
     9661 aaaggaaagt ctcaaagaat agtttatgat gctttcgcaa ttgttgctga aaagacagga
     9721 gaagaagctt tagaagtatt taataaagca atggataata taatgcctgt tttagaagta
     9781 aaagcaagaa gagttggtgg ggcaaactac caagtgccaa tcgaagttag acctgaaaga
     9841 agacaaacat taggtttaag atggctagta aaatatacta gagctcgtgg agaaaaagga
     9901 atggttgaaa agctagctaa agagataatg gacgcagcta acaacactgg agcttcagtt
     9961 aagaaaaaag aagatactca taaaatggca gaagctaata aagcatttgc tcattacaga
    10021 tggtaggata tatagatatc ttataaaatt gaggatatca taaggaggat aaccatggct
    10081 agaaagtttc ctttggaaag aactaggaat ataggaatca tggctcatat agatgcagga
    10141 aaaactacta ctacagaaag aatcctgttt tatactgggc aaactcataa aataggagaa
    10201 actcatgaag gagcttctca aatggactgg atggagcaag aaaaggaaag aggtataaca
    10261 ataacttctg ccgctactac tgcttcatgg aaagaccata gaataaacat aatagatact
    10321 cctggacacg tggacttcac agttgaggtt gaaagatctc taagagttct tgacggttca
    10381 gttgctgtat tctgtgcaaa aggtggggtt gagcctcaat cagaaaatgt atggagacaa
    10441 gctgaaactt atggagtacc tagaatagct tttgtaaata aaatggatat attaggtgca
    10501 gatttctaca atgttgtaag catgatgaaa tctagattaa attctaatgc agttcctatg
    10561 caattaccaa taggtaaaga agattcattt ataggtataa tagacttatt aaaaatggat
    10621 gctgttatat acaaagacga cctaggggtt gaaatggaag aaacagatat accagaagat
    10681 atgaaagaat tagctgctga gtggagagaa aaattagttg aatcagtagc tgaaactgat
    10741 gaagaattaa tgatgaaata ccttgaaggt gaagaactta ctatagatga attaaaagtt
    10801 gctataagaa aagctactat agcttgtgaa atgaacccag ttttctgtgg tacagcttat
    10861 agaaacaaag gtgttcagtt agtaatagat gcagttttag attacttacc agcgccaact
    10921 gatataccag ctataaaagg tatattagca gatggagaag aagctgaaag acattcttct
    10981 gatgaagagc cattctctgc attagcattc aaaataatga ctgacccatt cgttggaaaa
    11041 ctagcgttct tcagagttta ttctggaaca ttagaatcag gttcttatgt tttaaatgca
    11101 actaagaata agagagaaag aattggtcgt atattacaaa tgcatgctaa tacaagagaa
    11161 gaaataacta aagtgtatgc tggagatata gcagcagcag taggattaaa agatactact
    11221 actggagaca cattatgtga ccctgctaat ccaataatac ttgaatctat ggaattccct
    11281 gagccagtta tatctgttgc tatagaacca agttctaaag cagctcaaga aaaaatgggt
    11341 atagctcttc aaaaattagc tgaagaagac ccaactttca cagttaagac tgaccaagaa
    11401 acaggacaaa ctataatatc tggtatgggt gaattacact tagagataat agtagataga
    11461 ttattaagag aatttaaagt agaagctaaa gttggtgcac cacaagttgc atatagagag
    11521 actataactc aaccagttga tgttgaatac aagtactcta agcaatcagg tggtagagga
    11581 caatacggtc acgttaagat tagagttgct cctcaagagc caggtgaagg atacaaattc
    11641 acaaacaaaa ctgttggtgg atcagtacca aaagaatatg ttggtccagt tgacatgggt
    11701 atacaaggag ctatgcaatc tggtatagtt gctggatatc cagttgtaga cgttgctgtt
    11761 gagttatatg atggttctta ccatgaggtt gactcttctg agatggcgtt taagatggct
    11821 ggttcaatgg cattcaaaga tgctatgaag aaaggtaatg cagttctttt agagccttac
    11881 ttcaaagtag aagttgttac tccagaagat tacatgggag atgttatggg tgacttaaac
    11941 tcaagaagag gattaatcca aggtatggaa gctagaagtg gtgcacaagt tataaatgca
    12001 ttcgttccac tttcagaaat gtttggatac tcaacagacc taagatcttc aactcaaggt
    12061 cgtgcaactt acacaatgat attcgatcac tatgagcaag ttccagcttc agttgctaag
    12121 aaaatagctg aaggaaaata atttaaaaat aattaataat ataaatataa aaaataatca
    12181 gctagatgag agatatctct catctagcac aagat
//
LOCUS       DAMJWS010000105        12108 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMJWS010000105 DAMJWS010000000
VERSION     DAMJWS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.109"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            150..1541
                     /locus_tag="PC399_003259"
     CDS             150..1541
                     /locus_tag="PC399_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2594089.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1794..3587
                     /locus_tag="PC399_003260"
     CDS             1794..3587
                     /locus_tag="PC399_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2594090.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3668..5050)
                     /locus_tag="PC399_003261"
     CDS             complement(3668..5050)
                     /locus_tag="PC399_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2594091.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5308..5655
                     /locus_tag="PC399_003262"
     CDS             5308..5655
                     /locus_tag="PC399_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2594092.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5673..6707
                     /locus_tag="PC399_003263"
     CDS             5673..6707
                     /locus_tag="PC399_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594093.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6756..7805)
                     /locus_tag="PC399_003264"
     CDS             complement(6756..7805)
                     /locus_tag="PC399_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2594094.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8080..9615
                     /locus_tag="PC399_003265"
     CDS             8080..9615
                     /locus_tag="PC399_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594095.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9800..10486)
                     /locus_tag="PC399_003266"
     CDS             complement(9800..10486)
                     /locus_tag="PC399_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF2594096.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10611..11993
                     /locus_tag="PC399_003267"
     CDS             10611..11993
                     /locus_tag="PC399_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2594097.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
BASE COUNT         4611 a         1478 c         2016 g         4003 t
ORIGIN      
        1 taattttaaa aataataata aaaaaatgaa aatacaggcg ataatctatt gagtaaacat
       61 agaaaagatg atattataag tatgtgctaa aatggttaaa gcaatattaa tattaaattt
      121 aactataaaa caaaaggggg cgttttttta tgtcaagtac agaaagcgta tctaaaaaaa
      181 gtggtcatcc gaagagtttt tggcttattt gcatgacaat tttgtgggaa agatttagtt
      241 attatggttt aacagcttta ttggtattat tttttacagc aagtgtagct caaggtggtt
      301 taggtctttc tactcaagaa gcaacatcca tgtttggctt atttacaggt cttatatatc
      361 ttacaccatt agtcggtggc tggttagcag ataaagtttt aggtcaacaa aaatgtattt
      421 ctataggtgc acttttaata gcaataggag atttcgtact atttgcttca tctacatcta
      481 ttgcaatggt ttgggtaggt ctatcaataa ttattttagg aaatggtttt tttaaatcaa
      541 gttgctcaaa tatagttggg gatatatatc ctaaaactga tgtgtcaaga aaagatgcag
      601 catacagttt attctatatg gcagtaaatg taggagcatt tatagctcca ataatatgtg
      661 gattgctttc tgataatgta tttgcagtaa gaggagcaag tggagatata gtatcttatg
      721 gatataagat ggcattttta atttgtggta taggcatgat gttaggtatg attatattta
      781 cacttcttgc accaaaatta ttaaatgaag tcggaaaata tcctgtgact aaaggtaata
      841 aaggtgaaaa aattgagtat ggaccactta ctaagttgga gaaaaatagg ataatagcaa
      901 tggttatttt atttttattt gtggtcttat tctggacagc atactatcaa acacaaagtt
      961 cttttatgat ttatgtaagg gataatgtaa atagaactct atttggattt gaaatgccag
     1021 tagcatggct tacatcacta aatgctatat tatgtgtggc tttatgccca gtattagcta
     1081 ccctatgggt taaattatct cataccaaaa gaggtgattt ctctattcca gttaaaatgg
     1141 gattaggaat gataataatg ggtgttgggt ttatagttat ggtatttgct acattagcta
     1201 atggtccaga tggaatggta aaggctagta tattgtttat agtattgact tatatattta
     1261 caacagtagg tgaattattc ttatctccta taggacttgc aatgttcaat aaattagctc
     1321 cagctaagta tgctacgttg tctatgggag cctggtatct aacaagtttt tttgcaagtt
     1381 tattatcagg aaagattgct ggttttacag ccacacttgg ttttttacag atatttggag
     1441 gaatagcaac agttttaata atatttggtt taatattatt gggaataaga aatgtattaa
     1501 caagattaat ggctatggat ttactaacaa aagaaaaata attcatgacg aattattgta
     1561 aataaatgaa ttactttaaa taaaggaagt tacatttaaa tataaaatta aatgtaattt
     1621 cctctttgat tatgaaaaaa agctttacac aaatagatat tataattaaa attataattg
     1681 tgataattag agtgtataag atttatataa attatacaaa aaatatttat gtaataaact
     1741 ttacatactt atatatttag ttattcaaac tattatttcg aggaggtctt gttgtgaata
     1801 taaaagatag attaagtgga ttaagaaaat ttatggaaga aaaaaatata gatgcctata
     1861 tgattccatc ttctgataat caccaaagtg aatatgtagg agattacttc aaatcaagag
     1921 aatttatatc agggtttaat ggttcagcag ggacggttat tgtgactaaa gacgaagctg
     1981 ggttatggac agatggaaga tattttatac aggcagaatc tcaattagaa ggaagtacaa
     2041 taaaattatt caaaatggga caagaaggtt gcccaactac agatgaatat ttatataaaa
     2101 atattcctga aggaggaact ttaggatttg atggtagagt tatatctgct agagaaggag
     2161 ctacattagc tgaaaaatta tctaaaaaag gtataaagat agaatatcag tatgatttaa
     2221 tagatagtat atggccagat agacctgctt tatcagatag taaagcattt ttattagatg
     2281 ttaaatattg tggagaaagt tttagctcaa aattagctag attaagagaa aaaatgagtg
     2341 aaaaaggtac ttctactcat gtaataacta ctcttgatga tatagcatgg ttattcaata
     2401 taagaggtgg agatgttaaa tataatccag ttgtactttc ttatgcagta ataactttaa
     2461 aagaagttta tctatttgtt gatgaaagta agttaaatga agaaatacta aatgaactag
     2521 ctaaggaaaa tgttcaaata aagccatata atgatgtgta tgaatttgta aaaaatatag
     2581 acaaaactga aaaagtttta ttagatggaa ctaagcttag ttatacaatt tacaataata
     2641 taccttgtga agttgagaaa gttgatgagt tcaatccagt aatgtttttt aaagctcaaa
     2701 aaaatgaggt agaattagaa aatataagaa attcacatgt aaaagatggt gtagctttca
     2761 ctaaatttat gtactggtta aagaaaaatg taggtaagat ggaaatcact gaaataagtg
     2821 ctactcaaaa attagaggac ttaagaagag agcaagaagg tttctttgag ccaagtttca
     2881 atacaattgc tgcatataaa gagcatgctg caatgatgca ctatagtgca actccagagt
     2941 caaattataa attagaggca gaaggattat ttttagtaga ttcaggtgga caatattatg
     3001 atggaactac tgatataact aggactacag ttttaggacc tataagtgat gaattaaaac
     3061 tacactttac ttctgtagca agaggaatga taaatctttc taaggctaag ttcttgcatg
     3121 gttgtagagg atataactta gacatactat ctagaagttg catgtggaat atgggaattg
     3181 attatcaatg tggaactgga catggtatag gatttgtgct aaatgttcac gaagctccaa
     3241 atggatttag atggagagta gtacctgaaa gatttgatag tgcagttctt gaagaaggaa
     3301 tggtaactac taatgaacca ggtatatata tagaaggttc tcatggtata agaacagaga
     3361 atgaaattgt agttagaaaa gctgaaaaga atttctatgg tcaatttatg gaatttgaag
     3421 ttgttacgtt agctcctatt gacttagatg gtatagtacc agaattaatg aataaggatg
     3481 aaaaagatta tttaaactgg tatcataagt tagtttatga taaaatatca cctttcttaa
     3541 ctgatgaaga aagagaatgg ttaaaagttt acactagagc tatatagttt gtactagata
     3601 caataaaaat cgaggtatta taatgcctcg attttttatt gtatatttta ttaactttaa
     3661 aaaattttta atttgataat tctactaatt ttttattcat tataagtagt ataattccaa
     3721 atatcaataa aaatgatggt atcactatga agacttgtaa cataccaagt tggtctataa
     3781 tagcttgcgt atagccagat aatttatttg ctgcaaattt gcctaaaaac caaacaccca
     3841 ttaggaatga aatatacttt tcaggcgaaa gtttatttac cataccatac ccgactggag
     3901 aaaagcaaat ttctcctata ctttggaatg ttaaaaatcc aactacccaa aatacacttg
     3961 ctttgtgaat tgaattaggg tctgttcctg tttgtataac tgctactatc ataaacaaaa
     4021 atgctatggc taatagtata aaacctaagc tcattttttt tggcacactt aaatcgcctt
     4081 ttttagtttg agataatttt acccatacta aagcagagat aggtccaaga ataacacaaa
     4141 ggagaccatt gtatgaatct atccatgaag taggaatttg aaaattgcca acagtaagat
     4201 ttatgtgttc ctttgtatag agtgcaatag acatagaggc ttggttatat gctatccaaa
     4261 aaaatactgt gaaaaaagct aacaccaata ttactattat tctttttttc tcaatcaaag
     4321 ttaaaggtct tgaagctagc attttcttat attctagtga ttttcttata gactctctcc
     4381 cgttactagg gtcattattc aaaggtttat aaaactttct gtttccaaat ataaagaaga
     4441 ataaacctat tagaaccgta attgatgaaa ataaaaatac atatctatat ccatacatac
     4501 ttatagataa tccgcttttc atagcaaacc attcattagc aactaaaccg catattaaag
     4561 ggccaaataa ggtacctaga ttagtagcca tatacgtaat agagaaagct ccatcttttc
     4621 ttgggtcatc attggtatat aagtttccaa ccatagtatg taagtttcct ttataaaaac
     4681 ctattcctaa tccagataaa aatatcatga tgtttatatc catcttacta tttgctatgt
     4741 atcctagaaa ataaccaatt gaaaccatta gcattccaag tataatacag tttttgggac
     4801 ctaaaaatct atcactgacc catccaccaa taactggaga gaaataagtc catgctaaaa
     4861 aagatgcaag cattgatgcg gattctgctg gtgttaaccc taaaccacct ttggagactt
     4921 gttcagctaa aaacaataat aataccaatt tcataccata gtatgcaagt cctacaaagg
     4981 taaatattga acagcagaaa tataaatctc taggttgttt aagtttttct tgcgttttta
     5041 aaactgacat atgtcgagaa cctccagata aatagtttaa caaaatttta ccatactata
     5101 tgagtatatg tttttttcat atagacgtca acttgtaaga taatttaatt aagtcgaaat
     5161 atatgtaaaa atagtatcat ttttgtgtat tcagtaatat ttttttatga taatatatgt
     5221 tattattata ttaaaataca aacatattta tggaaaggat atgccaattt agtagttaga
     5281 ttgattgttt ggcaggtggg atgagctatg ataagaaaga aatctgaatt acaagtagaa
     5341 gtaaaagaaa atttaaatgg tggtatagga aaggttaagc ttgaaaacat aatacaaaat
     5401 gaagagctta aaggaaaagg aagactattt aaaagagtag ctttaccagt aggaagttca
     5461 attggtgtgc atgaccatac aacagatttt gaagtatatt atatactaaa gggaaaaggt
     5521 aaagtatttg ataatgggga atttgtagaa gtaaatgagg gtgatgtagt gtatacagct
     5581 gatggcgaaa aacattctat agaaaatata ggagaagaag atcttgagtt tgtagcacta
     5641 gttttatata tgtaaaatat ggaggaggag tcatgacagg caagatagaa gaaattaagg
     5701 acaagataaa tagattttgc aactccaatt taaatcaaga atacaaagat ataagcttta
     5761 aaattttgca aaacctttta gataataata aagagataat tcattcttct agagctgata
     5821 tatggtcttc agctatacta aatatagttt tagaacaaaa tcttttatac aataagaagc
     5881 atccactaca tataacaaaa aaagagtttt caaaacaaat aggtgtttct ttaaatacta
     5941 taaataataa atcgtcttta ataaaagatg tttgtagtat tgacttttta aatcaagata
     6001 ctatagctat ttctaatgta gaattttggg taaaagagac caattccaaa tcaaagtctg
     6061 tagatttaga tttagaaaag aaaaaaatac tatataaaag atatattaag ctatcacaag
     6121 atgcgaatag tgatattgaa tcaattagat atatggagga agctgtaaat ataggtaaaa
     6181 gtatgattac taaagaagat aaagaacttg gattttggaa aggtttatct accagagcct
     6241 atatgatagc acttgaatcc cttgctagaa agttagaaag tattaataat ctaaaggagt
     6301 ccaaaaaagt attagaatat ttaatagaaa ttaatcctag tgatgagcag ggaataagat
     6361 acaaattatt aaatgtatta attagattaa atgatagaac ttcaataaat aaattatttg
     6421 aactttataa agaagaaaaa tctgctacat ggatgtacag taaagctctt tattacttta
     6481 aaaataaaag tatgtttttg gcaaatgatt ctattaaggt tgctagaagt aaaaataagt
     6541 acgttggatt atatttgtta gattggcgaa atgcttttgg tagagaattt gttacagagg
     6601 aagaaaaggc agaagctgtc tattactatg atgaaaacat agtgatttgg aatgaagtta
     6661 aaggttctat ggattggcta ttaaaaaaaa tgttggaatt ttcataaaaa aaactactcc
     6721 ttacttttat ataagtaaaa ggagtagttt ttaatttata catctagttt taaatcttca
     6781 ggttttatta gacctttttt tctcataaat tctgaaatca acaaagttac aatagcagga
     6841 agtataaaat taagtaaaag tataccaacc cacatagata tacctccttt tccagcactt
     6901 tgcatagcag ttactgttcc aaattgtcca accaatccac aagtacccat accagaacca
     6961 ataggaatat tctccatttt gaatattgtt gtagataatg gtccaagtaa tattgaagct
     7021 acagtaggtg gtatccaaat tttccaattt tttattatat taggtacttg caacatagat
     7081 gtcccaagac cttgtgccac aaggccgcca actccatttt ctctaaagct cataacagca
     7141 aagcctacca tttggcaaca acaacctaca gttgctgcac caccagcaag accacctaaa
     7201 cttaacatca tacaaatagc tgcactactt ataggtaatg taagagctat tccaacaagt
     7261 gcagagacta aagctcccat tattataggt tgcatagttg tagcttgcat tattatattt
     7321 ccaaatgaag tcataaaagc agaaacagct ggaccaataa gttcagctac aagtactcca
     7381 attagtattg ttacagcagg tgtaaccaat atatctattt ttgtttcttt tgaaactaat
     7441 tttccaacct ctacagcaaa aagtgtagcg acaaatgctc caacaggtcc tcctaattca
     7501 ttaccacaag ctccaacaag tgcacaagaa aatagtacaa gtggaggtgc ttgaagtcca
     7561 tacgcaatag ccactgctat agctggacca gtaacctgtt tagcaagagg gcttataact
     7621 tcagtaaaaa gagaaatatt gaaattatct cctaatgtat tgaaaattgt tccaattaaa
     7681 agggacgaaa aaagaccgag tgccatataa cttagtgcgt caataagata cctttgaaca
     7741 gaaatttcaa tattttttct tttagcaaat gatttgaaat ttcttttttt ataattgatt
     7801 tccaatttaa aatcctcctc atataatata agtataattt ctttttaatt aattatataa
     7861 cttttcagct gacaagacaa atgttatttc actaaaatta gactattgta atagtaagaa
     7921 attaacatta attttagttt taaattttaa atttatggtt tataattagt atagggatat
     7981 tctgaaatat aataattttc cagtttatcc caatatttaa cttaggtaga ttgcttacaa
     8041 aacaaatgat tatatattag gctaaaagga agtggataga tgatatttgt aaaaaataag
     8101 aaatacagaa aaattgccat aattatatct attcctatat tgcttgttgc gcagtactac
     8161 ctttttaaat ttggaatact tagaccaatg gtaaagggag tagaaattga aattgtagat
     8221 ggggaatatg ttaaagatat agataaatat gtagtaaaat taaatgaaac agttactttg
     8281 tctacaggtg aatatattaa ggttccttcg tatgctaaaa atccaaatat ttggtttaat
     8341 gtattggata actcagggac tttaaaaata gatggaaata aaatgacagc attaaaggtt
     8401 gggtattctt ctgtgggtat catgaaaaat tcaagagtat taaaaaaggc tactataaaa
     8461 gtagtagacc cagaggtaga atcactagat atggagatta atggggattt aaaacatgtt
     8521 ggtgatagtg ctactataga aagtacaata gaagttgatt atgataagtt taaaaaatca
     8581 tataagccag tttataagag tagcaatgaa aatattttaa aaatcaaagg aaataaagta
     8641 agtgcagttg gagttggtaa agctacaata tatgctaagt gtggaaataa agaaattgag
     8701 agaacattta ggattcaagc tagagttgct gaaataaata tggatgatat aaaaatagaa
     8761 gttgaccaga atgaaaaatt gaaaccagat ataataactt ctccacctgg tttagaacca
     8821 ccaaaaatat attatgagtt agtagattca aaattacctg tagaaagagc aataagttta
     8881 agtagtaatg gaaatgttgt agggcttaga gaaggtaaag atagggtgag aataacttgt
     8941 ggagataaat ctaaagtaat aacagtaaca gtagtcaaag aaactataac gaatgactat
     9001 atacaaaact tggttggttc acataaaata gtaaacaata aagttattat taaacttgaa
     9061 tgggattata tgaaagatgt ctttgattat gatatatatc ttaaagataa gttgtcagga
     9121 gaatcattta agaaaattaa atctataact ataaatgaaa gtgatttagg taagtctaac
     9181 aaggtatatg caactataga agtagatatg aagggtaatc aaaatataga ttttgatatt
     9241 tatgtcatag gtaaaaagga ttcagataca actaagccaa gtaatgtaat aaatattacc
     9301 aatggaaatg gaaattcaaa taatgataat aatattaaag atatgagggt agaaaatata
     9361 tcagctaatg ttgatagaga taataatgtc attagagtta actggagtag tatatctcat
     9421 aaggactgta cttacagtat atatgtaaaa aataatacta atggagatgg agaatttact
     9481 ctatatcaaa gtgatataca gggaaatgag catactataa gtattcctga aggagattta
     9541 aatctagata tttatgttgt tgcaaattat aatggaaata gctctcaaaa tagtgatgtt
     9601 ataaatgtaa gatagatttg tatacaaatt gaaagtgttg agtttattta aatattaaat
     9661 taagaaaaag gatagtatag ggtaattata ctatcctttt ttgttacaat atataggttt
     9721 aaaatctcta gtaatataaa ttataaaaag tcttatttac acaaatctat agatattttt
     9781 tttattaatc aatattgtct taaattgaac ttagtgcggc ttctattgca tgtattactg
     9841 tcgtatcaaa tagtggaata gaagtgtcat tttgtttaat taaaagacct atttctgtac
     9901 atcctaatat aacagcctct gcaccttgtt tgccaagctt atctataata gataagaaag
     9961 ctttttttga agattcagat ataattccta agcaaagttc attaaatatt gtgtcattta
    10021 ctattatacg gtcttcttca tttggaatta agacttcaat tccattatta ataattacat
    10081 ttttataaaa atcttgttcc attgtatatt tagtacctaa taaggccact ttttttattt
    10141 ctttttccct taataccgta gcagttacat ctgctatgtg aagtaatgga atatgtatac
    10201 tttcttgtat tttatctgat accttatgca tagtattagt acagataaca ataaaatctg
    10261 cccctgcctc ttgtaattta attgcagcat cagctaaaat tttggcgctt ttttcccaat
    10321 ttccactaga ttggcattct tcaatttctt gaaaatctac actgtaaagt atacatttag
    10381 aagagtgtag tccacctaat ctttctttta ttactgtatt aataacttga taatatgtaa
    10441 tggtactttc ccaactcata ccaccaatta agcctatagt cttcatatta tacctctctt
    10501 atttatttat tttgaaatag ctatttacaa atacaagtat agcatctaaa atagaagcat
    10561 acaataataa aattgtatgg agtacaatta taattaagag gagtaattat atgccaataa
    10621 attcttttga aaactatcct atgacatgga aaccaaagtt agataaccga aaaccaccaa
    10681 tatataaaac tttagcaatg ttattagaag aagatattaa aagaggaaat ttaaatcctg
    10741 gagataaatt gcctccacaa cgggaattag cagattttct agatttaaac ttaagtacaa
    10801 taacacgagc atttaaactt tgcgaagaaa agggactaat atgtgcaaaa gtgggaaagg
    10861 gaacattcat ttcttctgat gtaaatgtaa gtaatacatt attatatcaa acggaatcaa
    10921 aagacataat tgagttgggg acagttcatc ctccgtatga gcaaaataca tatattattg
    10981 actttattaa aaatgtacta aaacaaccag aaatggatag gtttttacaa tacatgtctc
    11041 catctggcac atatatgcaa aaaaagtcaa ttgcaaaatg gatagaaaga aataatgtat
    11101 acaccagtga agaaaatata ttattatcta caggagggca aaatgctata tgtgcaactt
    11161 tactaggatt atttaaagct ggtgatagaa ttgctacaga ttctttaagt ttttctggaa
    11221 ttaagtcaat agctaaaatg ataggtattc aattagtccc aattcctcaa gaaaataatg
    11281 aaatatcgat tgaatatctt gaaagttatt gtaaaaatga aaatataaaa ggcatatatc
    11341 ttataccaga ttatcataat ccaacgacac atactatgtc tgatttatct cgtaaaaaga
    11401 tagccaagat agcaaagcaa tataatataa taattattga agatgctata aatagcattt
    11461 ttagagatgg tatacaaact cccatatttt ctttagctag tgataatact atatatattt
    11521 ttagtacatc taaattttta tgtgctggtc ttagagtagc atttgtagtt gctccaaaac
    11581 gatatataga aaatttagaa aatgcactat ataatatgaa tctaatggtt tctccattta
    11641 cagcagaaat agtacaccgt ttattatatt ctcctattat agataaaatt atagaagaga
    11701 aaagagatgc aattatagaa agaaatgaag ttgctgataa agttttgtca gattataatt
    11761 tgattggtga taagaattgc agttttcgtt ggttacttct tcctgatgaa ttggatggaa
    11821 aatcatttga aatttctgca aaaaatctag gagttcaagt atattgtgcc gaacgatttt
    11881 cagtaggaaa ctctacagtt ccaaaagcag ttcgtatttg tgtaacagct ccaaaagatg
    11941 tagaagaatt agaaaaaggg cttaatataa taaagtcact tcttaagagt taaatttatt
    12001 ttactattat ttaattaaat aaataaaaag gcggagaaat ttattataat aaacctccgc
    12061 ttttattatt taatgaaata ctttactttt tattaaaaaa tttaattt
//
LOCUS       DAMJWS010000106        11825 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMJWS010000106 DAMJWS010000000
VERSION     DAMJWS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.110"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            183..1223
                     /locus_tag="PC399_003268"
     CDS             183..1223
                     /locus_tag="PC399_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2594098.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1579..2901
                     /locus_tag="PC399_003269"
     CDS             1579..2901
                     /locus_tag="PC399_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2594099.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSIITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3198..4559
                     /locus_tag="PC399_003270"
     CDS             3198..4559
                     /locus_tag="PC399_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2594100.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTAIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4632..6026
                     /locus_tag="PC399_003271"
     CDS             4632..6026
                     /locus_tag="PC399_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2594101.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6088..6558
                     /locus_tag="PC399_003272"
     CDS             6088..6558
                     /locus_tag="PC399_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF2594102.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6561..8777
                     /locus_tag="PC399_003273"
     CDS             6561..8777
                     /locus_tag="PC399_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2594103.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8860..9531
                     /locus_tag="PC399_003274"
     CDS             8860..9531
                     /locus_tag="PC399_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2594104.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9894..11108
                     /gene="dpaL"
                     /locus_tag="PC399_003275"
     CDS             9894..11108
                     /gene="dpaL"
                     /locus_tag="PC399_003275"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2594105.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11235..>11825
                     /locus_tag="PC399_003276"
     CDS             11235..>11825
                     /locus_tag="PC399_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2594106.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4470 a         1363 c         2399 g         3593 t
ORIGIN      
        1 gagcattttt gtaaaatgaa gaaagtatat ctatcaaatt aaaactaagt agatatactt
       61 tttattaagt tttgttttaa aataatatta ttaaattata actaagcttt tctgttatga
      121 tataattaaa tagataatgt gaatgattat tatatagaat acttatttgg agttgatatt
      181 ttatggacaa acacagtgat tcagtaatgt atggtggaat ggaagtatat aatatttggg
      241 gaacaacaac agtagactcc ataattggag agtctgatgt aatgcgtgct cttaagaata
      301 gaatttgtaa aattggaaat agtgattcta ctgtagctat aactggagaa agtggcactg
      361 gaaaagaatt aatagcaaga gccattcatc tcgcaggaaa cagagggaat aaaacattcg
      421 ttgctataaa ctgtgctgct ataccagaac ctttactaga aagtgaactt tttggatata
      481 caaaaggagc ttttagtgga gcagattcaa aaggtaaaat tggtaagttt gaattggcca
      541 atggaggggt tgtattttta gatgagatag gagatatgcc aattcaacta caatcaaagt
      601 tattgagggt attacaggaa aagaagttta cgagaatagg ttcaaatgaa ctaattgata
      661 tagatgttag aatcatatca gctactaata gagatttatt tgagttggtt aaggaaaata
      721 aatttagaga agacttatac tatagattga atgtaatacc tttagaagtg cctccactta
      781 gagaaagagg agacgatata caaatattaa taaacaattt tatagacaaa tattcgagaa
      841 agatgaagaa aaacgtgtac catatagagc cagatgtgga acaaatgttg cttaagtatc
      901 cttggccagg aaatattaga gagttggaaa atactataga actggcatta aaccttcttg
      961 aggaagatgg aataatacat aaaggaataa taaatagaaa gatacttgaa tattttaagt
     1021 taaataatat aaatataaag ttacttgagg aaagtgacta tgagataagt atggaacaag
     1081 acatacttac acttgaagag tttgaaagag cctatataaa gaaagtgtta aagttttacg
     1141 gaaatgatac taagtctaaa aaactggctg ccaaaaaact tggaatatca ctagctacat
     1201 tgtacagaaa aatagatgta taataattag gctttttatt ctcaattcta aaatttgaaa
     1261 ttataaaatt gataaaaaac acttggaaaa ctattttcta agtgtttttt tgtgtaatag
     1321 cattttatta tccaaaaata aaaaaaattt aaaagaattt aaaaaaataa atgctttaat
     1381 aaattatcaa atactaacaa atataataaa aattatatat gtaaaacaat tatgttaaaa
     1441 aattggcatt ataattgctt catattaagt tgagatgaca aaataaaaaa agaaaggatg
     1501 ttgtgtgaga taataataac tgtcattaaa aaatagttgg gagtgataac cttgagtaaa
     1561 tatcaaaaga atgtttctat gtttgatatt gatggaaggc caccaatttt aaagtgtttg
     1621 ccactatcaa tgcaacatat actagcaatg atagttggaa ctgttacagt acctatagtc
     1681 gtaggaggag ctgtgggtgt tagccctaca caaataacac ttttagtgca atatgcatta
     1741 ataatagctg gtatatcaac acttatacaa ctatatccaa taggaaatat tggttcaaga
     1801 ttgcctataa tttttggagt tggttttact tatgtaccaa cacttgtagc agtcggagga
     1861 aattatggat tagcaagcat tttgggtgca caattaatag ggggagtggc tacagtatta
     1921 atagggatat tcataaaaaa gataagaaaa ttttttccga atatagttgc aggaacagtg
     1981 gtttttacaa ttggattatc tttgtatcct atagcaataa attatatggc aggtggtgta
     2041 aatagtccta cttatgcctc tatgcaaaac tgggcagtag cggctataac attagtggta
     2101 gttataggtc ttagtcaatt tgcaaaagga tatgcaaaat tggcagcaat atttttggga
     2161 attatagttg gatattgtgt atctttagtt ctgggtataa taaattttga ccctattaga
     2221 gaggcaactt ggtttgcgat acctaaaccg cttgaatttg ggatggaatt tgatatgcct
     2281 gtaataatct cagtagtaat tgtaagtata atcaatgctg ttcaagcaat tggagactta
     2341 tcagctacta caatgggagg catggatagg aatatcacag ataaggaatt atcaagtgga
     2401 gtaataggaa gtggaatttg tactatgata ggagctttgt ttggaggttt accaccatca
     2461 tcatatagcc aaaacgtggg gatggtagct atgaacaaag taataagtag atttgtgctt
     2521 ggaatagctg gtatatttat gttattgtcg gggtttgtgc ctaaatttgg tgcattaatg
     2581 actactatac catattctgt tttaggagga gctacaatat ctatatttag tataataaca
     2641 atgacaggta taaagttaat tattacagaa gagttgtcat caagaaatat taccattgtt
     2701 ggtttggcaa ttgccttagg catgggaata acatcagtcc cagcagccca agaggcattt
     2761 ccttcatttg taaagttagt atttggagaa tctccaatag taattgcaac attggttgca
     2821 ttcgttttaa atctaataat accaaataaa agtctagagg atgaggctaa agaaagagct
     2881 gttatggaag agactaatta agatataata atttaaaaga attgtttaaa ttatttagta
     2941 tgagattgtt agattatttt tgatagctat tcattttaat aaaaaattta acaaataaat
     3001 atataccagg caattaatgt tcatttaagt tgaaaatcgg ggaggcaata tttagaaatt
     3061 tgttaatttt ttataaaaat agctcagttt agctgtgttt atcggttgag caataaattc
     3121 atggtcataa aaatatttat agaaaataat tataaaaata tttagtcaag atatttatgt
     3181 atgggaggat ttattttatg aaaacacgta atacttcaaa atatgatgtc gatgggatac
     3241 caccactaaa ggaatcaata cctttggcct tacaacatct gttagcaatg atagtaggaa
     3301 atatggtacc tgcgatatta atagcaaacg tggtaggact taaccagggg caagcaacaa
     3361 tgttgattca gggttctatg ttagcagcag gtttagcaac gtttttacag ttatatccaa
     3421 tacctttatt taaaggattt aagttaggtt caagattacc tgtaatgatg ggaatgagtt
     3481 atgtattttt aggagcttgt ctttcagtag cagctaaaga tggtttagca gctctatttg
     3541 gagcacagat tgctgctggg gtaatcgtat tctttgtagg atttggagtt aagaaaatta
     3601 gacatatatt cactccaata gtttctggaa ctataatagc atgtatggga cttggactat
     3661 tcgcaacagc tataaagaat ttagctggag gagaaggcac acaaacattt ggaagcccaa
     3721 taaacttcac agtaggagtt atagttgctt ttgtaataat aatgataaat aaatacggta
     3781 aaggtttagt aaaaaattct tcaatattaa taggaatatt agtaggttat gctatatctt
     3841 tagttttagg attggttgat ttctcagccg ttcaaggagc agctatagta tctttaccaa
     3901 caccagcagc ttttggatta gaatttagac cagagttaat agtaatgttt actattattt
     3961 acataatagg tatagctgat atgatgggtg cctgtacaat agctacagta ggagcaatgg
     4021 atagagaggt aactgacgaa gaattagcat cagttgtatt aggaaattct ataacttcta
     4081 taatttcatc actatttgca gctcttccaa ctggagtatt tagccaaaat acagcaattg
     4141 tatctatgaa caaagttaca agtagatttg taatagcact aggggcactt gtattattac
     4201 tagcagggat atctccagca ttgggtgcaa taatgacaac tataccatct tgtgttgttg
     4261 gtggagcaac tttagtagtt ttctcatcaa tagcaatgtc tggtttctct attatgagta
     4321 tggatggatt cactgaagaa aacaatctta tagcaggtgt atctatagca acaagtatgg
     4381 gtcttacaac tgctccacaa gttttagacc aattcccaga aacaattgga actgtccttg
     4441 gagggtcaag tatagtttct ggagcaataa tagctatatt acttcaaaca ttatttaaat
     4501 taaagtcacg taaaagtgct gaaaacgtta cttcaaatct tgaagaaaat attggataaa
     4561 tgtattagat ataatatgag aaaaccatat ggtaaaatgt tgtaatgaaa gtattcaaag
     4621 ggggatttaa gatgattgat atactaataa aaaatgcaat aattgttaca gttaataaag
     4681 acagagatgt tatatttgat ggagcaatag ctattcaagg tgacagaatc ttggacatag
     4741 gaaatacagt tgatatcgag cctaagtatc ttgatgctaa aaaagttata gatgctaatg
     4801 gaaaagtaat attccctggc tttataaata ctcataatca cctgttccaa gtgttattaa
     4861 aaggtttagg tgatgatatg gcacttcatg agtggttaaa tacaatgatg tttccatcag
     4921 caaaattctt aacagaacaa gatacttatg atgctgcaat gcttggatgt atggaaggtt
     4981 taaaaagtgg tataactaca atggttgatt atatgcatac tcacaataga ccaggactta
     5041 cagatggaat agtaaaggca tataaagatt taggcataag aggggttgta ggtagaggtt
     5101 gtatagattt aggtattcac aaagagctta tagaagatgt ggaaacggtt gaaaaagatt
     5161 tgagaagggt attcgaaaaa tatcataatt cagaaaatgg aagaataaaa atctgtgtag
     5221 ctccttcttc aatgtgggct atatctgaag aaatgggtaa gatgctttgg aatatagtta
     5281 aagaatatga ttcatatttt acagttcata tatctgaaac agaatttgca agaacagcaa
     5341 caaaggatat acatggagaa atagatataa aattacttga aaaatggggt atagtaggtc
     5401 cagaagttgt agcagtacat tgtgtatgta taactgatga agacatggaa atgcttaaaa
     5461 agtatgacat aaaagtttct cataatgtag caagtaatat gtatcttgct tctggtgttg
     5521 cacctgtgcc agaaatgtta aaaaaaggta taaatgtgag tttaggtctt gacggtgcag
     5581 caagtaataa cgcacaagat atggtagagc ttatgaaact aacagcttta caacacaaag
     5641 taaataattg tgaccctctg gcaatttctg cagagaaagt tcttgaaatg gctacaattg
     5701 agggagcaag aactcttaga atggaagatg aaataggttc tttggagatt gggaaaaaag
     5761 ctgacctagt aatatttaac tcaatgcttt ctccaaaatc aatacctctt cacaatcctg
     5821 tatcaacttt ggtatactca gctagtatgc acaatgtaga gagtgtttta gttgatggaa
     5881 atgtaatact tgaaaatgga aaagtaacta caattgaaag tgaagaacaa gtgttgctta
     5941 atgcacaaca tgcagcagaa gaattatgta agagagctaa tgtaactaat agaatggaag
     6001 gacataagtg gaatagctta tactaatatt aaaataagat tgtaataata ttctattaca
     6061 acattagttt atctgtagga ggagaaaatg gaaaaaataa aattaaatat taatgattac
     6121 atgtatgaaa tagaagttga aaagaatgaa actcttttgc atgtacttcg tgaaaaatta
     6181 aatctaactg gagcgaaaca aggttgtaat agtggcagtt gtggagcatg taaagttttg
     6241 atagatggag ttgacatgaa atcttgtaaa cttcttgcaa aaaactgcga aggtaaagtt
     6301 atatacacta tagaaagttt tgctaatgga gaaaatcttc atccaataca agaagctttt
     6361 atagaagttg gagctgtaca atgtggatat tgtactcctg gtatgattat aacaacacaa
     6421 gctcttttaa atagaaatgc atctcctaat gaagatgaaa ttagagaagc ttttaaagaa
     6481 aatctctgta gatgcacagg atatgtaaag attatagaag ctgtaaaatt atctgcagct
     6541 agattagggg gtagataatt atggaaaagg taataggaaa atcataccct ctaaaagatg
     6601 cagctttaaa agttactggt caaatgaaat acgtggctga tatgaaacca caaaatgtac
     6661 tttacgctaa gatgctttta agccctgtag cacatgcaaa aataaaaagt atagatacaa
     6721 gtgaagctga gaaagtagta ggtgttagag ctatagctac ttatctcaat acatcacaaa
     6781 gaaaatacaa tggttcatta agattttatg agcacaaaat acctgaaaat gaagttatat
     6841 tttcagatac agttagattt gttggtgacc gtgttgcagc agtagctgca gagactccag
     6901 aaatagcaga gaaagctgta aaacttataa aagttgaata tgaagaattg ccagctgtat
     6961 ttactataca agatgcaata aaagaagatg ctatctgcat acatggagat tcaaatatag
     7021 tttctcaaaa caacataaat gggggagatg tagaaaaagg cttcaaggaa tgtgactata
     7081 tatttgaaga tgaatactct actccagcaa tacatcatag tgcaatagag actcatagtg
     7141 taattgcaca ctatgattat aatgataaat taactgtaca tacaccttgc caaaatacat
     7201 ttggatttag aataatacta tctcaaattt ttgggatgtc attaaataaa gtaagagtta
     7261 taagacctgc tattggaggc tcatttggtg gaaaattaga acttacaatt gaaccagtag
     7321 cagccgcatt atcaaaaatg acaagaagac cagttaaaat ggttcttaca agaaaggaat
     7381 ctatgatatc aacaagaact cgtcatgggt cttttgtaaa attaaaaact ggtgtaaata
     7441 aagatggtga agtaatagca caagacataa aaatatatac aaatacagga gcatatgcat
     7501 caagtgcttt aaatgttata ggagcactta gtcataaagt atttaaagtt tataaaatac
     7561 caaatataaa gtttactggg atgcctgtat atacaaatac accaatagca ggagctatga
     7621 gaggctatgg gtcacctcaa atatttatgg ctcaacaagc tcaatttgcg aaaatagcaa
     7681 aggaaatagg tatggattta gtcgattttc aaaataaaaa tgcagtagag ccagatgatg
     7741 tcgatataat cttccacggt tctcttggaa atcctagagt attggattgt atagagcaag
     7801 gtaagaagat gtttaagtgg gatgaaaaga aaaaacttcc aaaagaagaa ggaaaatatt
     7861 taagaggtat aggtatggca ataggtgcac atggtaatgg agtttttggt gcacatagag
     7921 atgttgttac attgactctt aaattaaatg aagatggtac gttgacactg ctaacaggag
     7981 ctcatgatat gggtaatggt gttgtaacta tgcaaacaat gatggtagct gaagttttag
     8041 gaataacacc tgataaagtt gatacatttg aaactgatac ggatgcatgt acattcaacc
     8101 ttggtgacta tgcaagtaga ggggtatttg tagaaggtgg aggagctaaa aagacagcag
     8161 agaaattgaa aaatcttata ttagaagaag cagaaaaatt acttgaaaca tcaaaagaag
     8221 atttatatct agacaatgga tgtgtaatta gtaaacttga tgaaaatgta aaagcatcac
     8281 tttctgatgt cgcagtatat agtcaaagta aatcattaag agagttaact gttgttgaag
     8341 attattcttc accagctgga cgtacttctt atggtgtaca ttttgctgaa gttttagtag
     8401 ataaagaaac taaggatata aaagtagttg attttgtagc tgtacatgat gttggtagag
     8461 ttataaatcc tctaaattta gaaggacaat tagaaggtgg gattcatatg ggtctaggat
     8521 acgcattgag tgaagaattg gcatttgatg aacaaggtag acctaaagct actgatttta
     8581 aaacatataa attatttaga actgtagata tgccaaaatg tcaagttgca tttgtagatg
     8641 gttatgaaaa agctgggcct tttggtggta agagtattgg agaatgtgcc gttgtaccag
     8701 ttgcgccagc tgttgcaaat gcagtatata atgcaactga tgtagagata cattcattac
     8761 caataaaatt aaaatagtat aaattaaagc agaaataaca atttattgta tttgtaaata
     8821 gataataaaa atagatttat ttggtgagga gatattatta tggcaaagat attaggtatt
     8881 tgtggaagtc cacgtaaagg agcaacagaa tatgcagtgt tagaagcttt gaaagaagca
     8941 gaaactatac ctggtataga aactgagtat tggtcagtta gagggaaaaa aataagtcct
     9001 tgtgtacatt gtgatgcatg tataagaaaa gaaactatgt gtattataaa agatgatatc
     9061 caagaattag agcaaaaaat attggaggca gatggaatta tagtaggttc tccagtttat
     9121 gatatgaata taactgctca acttacagca gtatttaata gacttcgtcc aatgtattta
     9181 gttcatccag gaaaacttca aaataaggtt ggtgcagcta taactactgg tggaactaga
     9241 tatggtggcc aagaattgac aaaacttcct ataataaatt tcttcctaat gcatgaaatg
     9301 ttagtgtctg gaggtttagg tggatgctat ataggtggta caatatggag taaagatgga
     9361 aaagcaaaag gtgcccaaga ggatgagaca ggtatggaca cagtaaaaag acttggaaaa
     9421 ggtgcagcag aggcagttat ggtttctaag tttggtcttg aaaaatggaa tgtcgtaaaa
     9481 gaagaattag atgttaaaaa agatgaaaaa tcacctctta gagaccacta aagatttatt
     9541 atattattga atatatacaa atagtgatgt aatagattat tgcatcacta ttttatgttt
     9601 taatttagat tttatagatt ttatattaaa aaatatagaa acaaagtgtt ttgagattta
     9661 tttttaaatt ttaaaaatga gaattaaaaa gtgagaagat agaaaaggtt ttttagttat
     9721 taaattctac atacattgta tgaactaaag atagaaaaga aaaaataata aaagcattgc
     9781 aatttctata ctaaagagtc atagaaaaat atctagtata tatttagtgt tggcatgtta
     9841 attgcttata tattaaagta taaaaattta acaataattt ggaggtattt tagatgttaa
     9901 aagaaattaa atggaaagta aataatttgc caaagggcga taaggaaaat tgtatcaaat
     9961 ttttaaatga agaagaaata actaaggtta gaaattttca caaaagcttt cctcaatata
    10021 aagaaactcc attagcaaac ctagaaggtt tagcaaaaaa attaggtgtt gcaggagtat
    10081 atgtaaaaga tgaatcttat agatttggat taaatgcatt taaagtatta ggtggttctt
    10141 attctatggg tagatactta gctcaaagat tagatacaga tatatctgag ttagggtatg
    10201 acaaattaac ttcaaaagaa ataaaagaaa aattaggaga aataactttc tttacagcta
    10261 cagatggaaa tcacggtaga ggagttgctt ggacagctaa taaattaggt caaaaatctg
    10321 ttgtattaat gccaaaaggt tcttctgaat ttagattaaa taaaattaaa ggagaaggtg
    10381 cagatgctag tataacagac ttaaactatg atgatgcagt aagattagct aatgattatg
    10441 cagaagctga tgaccatgga gtaatggttc aagatacagc atgggacgga tatgaagaaa
    10501 taccagcgtg gataatgcaa ggatatggaa ctatggctca agaagctata gagcaattaa
    10561 aagaatatgg agtagataga ccaacacacg tatttgttca agcaggagtt ggttcacttg
    10621 ctggtgcagt tcaagggtat gttgcttcaa tatatgatga atgcccaata acagtagtag
    10681 tagaggctga tgaagctgat tgttactata aatcagcaga agctggagat ggaaaaccaa
    10741 gatttgttgg aggagatatg ccaactataa tggcagggct tgcttgtgga gagccaaata
    10801 ctataggatt tgaagtatta aaaaatcatg cagcagcatt tgtttcagca ccagactggg
    10861 tttctgctaa gggtatgaga acattaggta atcctttaaa tggtgatgaa aaggtaattt
    10921 ctggagaatc tggagcagtt acaactggtc ttttagtagc agctatggaa agagaagatt
    10981 tagcagattt aagaaaagat ttaaaattag atgaaaattc aagaatactt ttaataagca
    11041 cagaaggaga tactgaccct gacaaatata gaagtatagt ttgggatgga gaatatccaa
    11101 gtatataagg aattactaaa atgcaattat tagatattgg tttacatatt tagaagatat
    11161 ataagttcta aaatttagaa aatatataag tatataaaat acatacatta taaaaaatct
    11221 agggggataa tgaaatgtta aacgaacaaa gaaaacaaga agtaactgaa atatgccaaa
    11281 aattaattca aaatcctagt tcttctggaa atgaagaagg tgttgtaaaa gcaatagaag
    11341 agtcttttga aaaattaggt tttgatagtt ggtcaagaga tagatatgga aatatagtag
    11401 gatgtataaa aggtaacaag ccaggtaaga aaatattatt tgatggacat atagatacag
    11461 ttccagttcc agatgcaagt aaatggagtg ttcctccttt tgaaggaaaa atagttgatg
    11521 gaaagatata tggtagagga acttctgaca tgaaaggtca atatactgca atgatgtctg
    11581 ctgtagcgta ttttgctaaa gacactaata gagattttgc tggagaacta tatgtagcag
    11641 gtgttgttca tgaagaaata tttgaaggtg tttcggctag agagataagt aaggctgttc
    11701 aacctgacta tgttgtaata ggtgagtctt cagagttaaa cttaaagatt ggtcagagag
    11761 gtagaggaga aatagtagta gaaacttttg gtaaaccagc tcactctgct aatccagaaa
    11821 aaggt
//
LOCUS       DAMJWS010000107        11492 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMJWS010000107 DAMJWS010000000
VERSION     DAMJWS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.111"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            235..417
                     /locus_tag="PC399_003277"
     CDS             235..417
                     /locus_tag="PC399_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594107.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            1249..2163
                     /locus_tag="PC399_003278"
     CDS             1249..2163
                     /locus_tag="PC399_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF2594108.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRYYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2553..3200
                     /locus_tag="PC399_003279"
     CDS             2553..3200
                     /locus_tag="PC399_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2594109.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3367..3936)
                     /locus_tag="PC399_003280"
     CDS             complement(3367..3936)
                     /locus_tag="PC399_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2594110.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3941..4840)
                     /locus_tag="PC399_003281"
     CDS             complement(3941..4840)
                     /locus_tag="PC399_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2594111.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGILLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4851..5000)
                     /locus_tag="PC399_003282"
     CDS             complement(4851..5000)
                     /locus_tag="PC399_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594112.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4993..5577)
                     /locus_tag="PC399_003283"
     CDS             complement(4993..5577)
                     /locus_tag="PC399_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2594113.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            5966..7291
                     /gene="cdeA"
                     /locus_tag="PC399_003284"
     CDS             5966..7291
                     /gene="cdeA"
                     /locus_tag="PC399_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF2594114.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFISSLLVFIIALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(7386..8180)
                     /locus_tag="PC399_003285"
     CDS             complement(7386..8180)
                     /locus_tag="PC399_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594115.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(8181..9056)
                     /locus_tag="PC399_003286"
     CDS             complement(8181..9056)
                     /locus_tag="PC399_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2594116.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(9060..9428)
                     /locus_tag="PC399_003287"
     CDS             complement(9060..9428)
                     /locus_tag="PC399_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2594117.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(9611..10270)
                     /gene="deoC"
                     /locus_tag="PC399_003288"
     CDS             complement(9611..10270)
                     /gene="deoC"
                     /locus_tag="PC399_003288"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF2594118.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(10306..>11492)
                     /gene="murJ"
                     /locus_tag="PC399_003289"
     CDS             complement(10306..>11492)
                     /gene="murJ"
                     /locus_tag="PC399_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2594119.1"
                     /translation="KQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSIP
                     YNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSLI
                     KLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIVS
                     VMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAFD
                     ARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLVL
                     IKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVLS
                     YFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLHL
                     KK"
BASE COUNT         4035 a         1752 c         1295 g         4410 t
ORIGIN      
        1 tattaattta tattaaatat ctactaactc aaatattcat aattaaactt taaactggat
       61 aaattttctt ttaagaatga aagtttatcc agttttgcga ttataataac cagttttaca
      121 tatccacttg taaaatatat ctaagcaata tatcatacta tatagattta tgagtgaaat
      181 aatatctgct atttactgaa tatttttatt tttcgttttt tagggggatt agttgtgttt
      241 aaagataaga ttgatgaatg tgttcatata atgactgcat atattgctag tttaaaagaa
      301 tactattcat tcatagaaac tcaaattggt gattttatta aaaaatatgg agaagatgtt
      361 gtagagttat gtctccatag agtgatgatt ctactatgcg aatgtggttt ggcataaaat
      421 taaatttatt aaaatgggta agaagaatcc tatataatat tcttcttacc cattttaata
      481 tatcaatttc atagcttatc gtttaaaata ttttacatcc attactagac aactttacat
      541 tctcttttta attctagtat atttaaatca ttttagttgc tccttattta ttcttattac
      601 ttgttttatt ttataaataa aataattctt tttaataatg ttttttgttg atattcctat
      661 cacacatcca attatttatc attgtcttga aattgtttgc atacttaatt taatccaatt
      721 gaaacacccc agcatagaat cccctgtaaa atagaagttg gattaaatcc taacatctat
      781 acagaaaata ctactccaag cacaagcaat ataatggaat atatttatca cttaattttt
      841 tatacttttt aaatcccata cctaaagcat taagtgcagc tattagaatt aataattgtt
      901 gtggtataaa attatgcttg cttcatattt attcattttt tactataaaa gttcttgttt
      961 tacaaaaaac atttgttttg tatcaaaata atacttgtaa attacataat tctcataaaa
     1021 atttctagta ttacttgtac tcttaataaa taatagccaa caagtacctt aaatcattat
     1081 atctatatct aataagaaac acagtaagat aattttttcg cccataactt aataaaaact
     1141 gttgatatta gtagcaattg aatttagtta atttatttat aatatacata aatttaaata
     1201 ttacgaataa agtattataa ataggatact ttctaaaggg aggaaattat gatagataat
     1261 caaaaatatg ttattttatc actagaatta catttatttt tttcaagaat tatgaaagag
     1321 catgctcttt ttttagaagc aggattcaca aataaaaatt ataatcttgc tatggaagct
     1381 gaccactata aaaagcaatt tgaagattta ttatcataca ctgttagtgc tagtaatggt
     1441 ataattagac ctgatatatt atattcagaa gagcttgtaa ctactctcac atcagttgca
     1501 gaacaaaaaa cagaagagtt tacagggata gaaataaaca aaaacatcac tacaagagaa
     1561 ttaaatctac aaagtggtgt aaacccacaa gttggtcaag acttagtgaa ctatgtagct
     1621 caacttaact ctgatgcaat aagattactt gatgggctta ttaattttaa agaaagagtc
     1681 ttagatggtg tactatcatg tactatattt acatcaaact accctctact tcttgaacat
     1741 ataatacatg aagcaaattt atatcgttat tatgtagttg accttgaaaa taaaatagat
     1801 attgagtcaa aaaacgctaa agaaatagaa ttattctggg accatattat gatggaacat
     1861 gctctgttta tgagaggatt actagacccc tcagaaggtg aactaataaa tacttcaaat
     1921 gattttgcta taaaatttaa tgaattaatt gaaaaaacaa acgaaatgac tgattctaat
     1981 atcaagaaca ttacagaaga aactctaaat gaaactgttg agtttaaaga ttttaaagaa
     2041 gcaggagcat caggaataga acagtgtaag ataaaatcta taatattacc acttttagca
     2101 gaccatgttt taagagaggc aaatcattat attagaatat tggagagtta taaaaacatg
     2161 taattaaaaa ttacttttaa aataatcttt gcatatttag ataaaataaa ggtacagtta
     2221 actgtacctt tattttttat aaacttataa aatttaaaca agttattcat aaaataaaat
     2281 aataatcatc tataacccac aactttattt tatattaaat aaaaagtatt agtgattatg
     2341 ttttataggt atttctttat tcagttattt attaaaaact aatagtaaat cttatactaa
     2401 tcgcatattt atgttataac tatattgtaa ctattttcgt aatcttagga aaagattact
     2461 tataaaatta accttataca ataatatatc gtataatctt aattagcata atagttacaa
     2521 tattaaaatt taaatatggg agtgatttta gtatgataag acaaacacca ttagagaaac
     2581 gatatgacaa gctaggtcct catattgtaa cagcactcaa aaaacgttat tttgatgctt
     2641 attactgtaa aactagaaat gaagctttgc aacaaatttt agatttaatt cctcaaaatc
     2701 atgtagtatc atggggagga tctgaaacac ttaaagaaat gggcgtacaa actgctgtta
     2761 gagagaaagg atttaaagtt attgatagag atttagcaaa aactccagaa gaaagaactg
     2821 aaattatgcg tcaagcactt ctatgcgata cttttttgat gagtagcaat gcaattagtg
     2881 aagatggaca actctttaat attgatggaa atggcaatcg tgtagctgca atgatatttg
     2941 gtccaaaaaa tgttatagtt gtagctggta tgaataaaat tgtaaaaaac attgatgatg
     3001 caatacaaag aactagaaca attgcatctc cagcagttgt tcagcgtttt caagatgtca
     3061 atacaccatg ctatattaat ggaagttgta tagattgcac aagccctgaa agcatttgtg
     3121 catacatggt tactacaaga atcagtcgtc cagcaaaaaa aataaaggta atattggtag
     3181 gagaaaatct tggtctataa aatatacata catgtaaata gctctataaa taataaaatc
     3241 atatgaaata acttatttct aaaagtggta gaataaaagc ttctacaaga ctttcattct
     3301 accactttta tattctttta atttgtgttt ctatattatt aattgctccg attttcatat
     3361 atattactaa ctatttttat tttcataatt tttttgttct tcatcaagct gtttataata
     3421 atctttatta tagtcagcta gataatctat accacctcct gtaatcacat ttaataattt
     3481 cattgcagat tcttgattaa ctttacaaac ataattatca ccagatttaa caagctctaa
     3541 tgtaatatta aattcttttt tctttgcaga atcaatatct ttcatagttg gatttggcat
     3601 catttctcct agtttaggag gattcttttt taaaatagaa accatatcag tattgttaat
     3661 ttttaaatct acagtcactt tatccttatt attctttata tctgaaattt cccaagtcat
     3721 atttttagaa tacattttaa atccttcttt atcttcgtat ataagattac ctaaccctga
     3781 ttttgggaaa tacttatcaa cagtatctgc atcttgtgtt tttattgaat taaacatact
     3841 atccacttca ttttttatct tattatcatt agtacaacca gctaacacca gtactgatat
     3901 tgcaaacatt ataatacata taataaattt cttcattaaa ttatatcccc ctatactttt
     3961 ttgcacaaat attattctct tttttatctt ttgaaaaagt tgtagtaatt gcattttgtg
     4021 gacaaacttt tatacattca ccacacctaa tacagtctgg actatttaca tctttatata
     4081 ttggtatatc aagcttacat tttgtttgac atgctgtaca atttgtacat ttatctttat
     4141 caattttaaa tctatatatt gaaataggat taaaaaaacc atagattgct cctaaagggc
     4201 atatatactt gcaaaatggc ctataagtaa tgattgacat caataatatc aatagcaaaa
     4261 tacttaactt ccagctaaat aaaaatccta ttgattctct taaattctca tttcctaaaa
     4321 taagtagtat tcctcccaaa agtgttcctg aagggcaaat ccatttacaa aaccaaggat
     4381 ttccaccccc tgctacattt acaatagcaa ctggcaaaat aattacaaac attattaaga
     4441 ctatatactt tagatatttt agatatttat caccataaac tttttttatc tttactggaa
     4501 agggtacttt atgtagtaaa tcttgtacaa gtccaaaggg acaaagccaa ccacaaacaa
     4561 atctgccaaa aaaagcacca aatgccatta aaaaaccaac aacataaaat gagaatttat
     4621 actctctgct tgataaaact gcttgtaatg aacctattgg acatgctcca agtgcacctg
     4681 gacatgaata acaattcaat ccaggtacac ataatttttt actttctcct ccatatatag
     4741 ttccttctaa aaatccattt acatatccat ttgttaatgc tgtaaatgca acttgagtaa
     4801 taagtcttat attattattt accttagata ataacttcat ccctcttctc ctaaccaata
     4861 ccaatacact caagacaaat cataattgct ttagttaata ctgtatttgt ttctccacga
     4921 tatgtaccta atgcaataaa tataatacta attgcaataa tagaaatgcc ttttttatta
     4981 tctttaataa agttactcat tggcttttac catttttagt ctttcatcta taattttttt
     5041 ccaatcttcc ttactatttg cacctacaac cttctcaccc acaaagtttc ctttcttgtc
     5101 tacaaaataa gttgttggaa actccataac tcttttaaaa tcagtttttt taagttcttc
     5161 agatacagtc aattgttggt aagttgcatc tgactttttc ataatgtctt taactaaatt
     5221 tttctcttca tttgaaagac cagttctcat atcagtttta tcaacttcta caactaatcc
     5281 ctttatagct acattggaat tattactctc atattctttt gataatgctt caagctctgg
     5341 catttcaccc acacatggtc cacaccaagt agtccagaca ttaataagag taagttcttt
     5401 atttgaaaat atctcttttg ttaccttatt tccatcaaca tcctccgtat ctaaattact
     5461 aaaccattca ctattattct tgtttttttc tactggctta gattcttttt tgctacatcc
     5521 agaaatcata cctaaaacac aaatacatcc taaaactact atgaaaaacc ttttcatatc
     5581 tcacactcct agaatttata tagtatataa aatcgctgct tgaaataaaa ataacttatc
     5641 ttctatatta aagtaattat atttaataaa taagagctaa tttaaaaaaa gatatacagt
     5701 taaaatctat ttcttttcag acaaaatctt atatatatat aatagcttgt aaatcttaag
     5761 attatataca tatatttcta aagattttct taagaaacag ttagatttta ctaatataag
     5821 tttattaatt agcattatac taatttatct taatatgatt ttttgttgat tttaagctgt
     5881 ttatcgtata aataaaattt ttcttgacta tatagttact atatagctta ttatatatag
     5941 agagtaataa aaggaggtaa aatttatgga aaatttattt acaagaaaat tcactacttt
     6001 tgaatttcta aaatttgttt ctcctgcaat tatatccatg atatttatat ctttgtatac
     6061 aataatagat ggcatctttg tatcgacatt agttggttct gatgctcttg ctagtataaa
     6121 tattgtacta cctataatta accttgtttg tggatttgga ataatgatgg caactggtgg
     6181 aggtgctatc gtttctatac gtatgggtga aaatagacag gatgaagcca actctacatt
     6241 ttcttttata gttttgtttt cattgattgt tgggatttta ttcacagtaa tctcatattt
     6301 ctttatcaaa gaaatatcta ttttgcttgg tgcaacagat aagttattac catattgtat
     6361 aacttatggt aaagttatga ttttatgtac accattttat attttaaaat ttatatttga
     6421 gtactttgca agaactgatg gaaattctaa atttagttta tttctatcag tcattggtgg
     6481 tgtaacaaat ataatattgg attatgtatt tattaaatat tttggaatgg gtcttttagg
     6541 agctgcagtt gcaactgcta taggtattat tttaacttgt gttttaggta ttatttactt
     6601 cttatctaat aaatctacac taaaattaag aaaaccaaaa accgatttta gacttataag
     6661 agatactatg ataaacggtt cttctgaaat ggttacagaa ttatctacag gaattacaac
     6721 attcttattt aatgtagtag ctttaaaatt agcaggagaa aatggacttg ctgctcttac
     6781 tatagtattg tatgctcatt ttttaatgac atcagtctat ctaggattcg ctgctggagt
     6841 gtctccatta ataagctata attttggtgc tgaaaacagt gataaattaa aagaaacatt
     6901 taaacattct ctaaaattta tatttatttc ttctctttta gtgtttatta ttgctttagt
     6961 atttgcacca tttattgtta gggtctttgt aaatccagat aacacagtat ttaaactagc
     7021 cttacaagga ttaaaaatat ttgcatttgc ttttttgttt gttggtataa atatatttgc
     7081 atcaggattt tttacagcat ttcacaatgg aaaaatttca gctattatat cttttagtcg
     7141 tgcctttgtt tttataatca taggaatcat aattcttcct cctatgttaa acatgactgg
     7201 attatggctt acagttccat ttgctgaggt tataaccata tttatatcta ttctatttat
     7261 aaaaaaatat aaatgtagat ataagtatta aaaattaata cttaaaatca acatttatta
     7321 aaactaataa acctgcttta aaatacaaat ttacaaattt gatttttaag caggttttat
     7381 tttagttata ataagtcatt tgcatactct ttaattggtg cttttctaag taataaaatt
     7441 ataaatatta tgcaaataac tataattcct acctttgttc caaataaaat aaaaggattc
     7501 tgaaatgaat aaataatttt ttctaaatac aatagcattt tatgcatatt tttagttgca
     7561 aaattaacta tataaattac cattaatatc ggtaatccta tcaatatagt aatactagta
     7621 acttttctaa ctctatatgt aaaagctcca agtaacactc ctataaaacc tatacatatt
     7681 tcatttatac atatttgtac aaaataattt aaataagtta gtggcatact tgatgttgaa
     7741 ggatctatca gtgctgatat agatgtccaa atcatatctc tctttagtaa aactgatagt
     7801 tctatactgt aacctgttat aatttctata aataacttcg ataaaaaaat caaaactata
     7861 cctaaaatag aaatcacaaa gcttaaaata attaaatcta tgacaatagc ttttatacaa
     7921 ctttttctat ctgctcttat acttaaagct cctgaaaagt ctttatttgc tatatttact
     7981 ccatacacaa atagcagtat agttatatat gttaaaatag gattcacaat cgtggataaa
     8041 ttagttttag atgcttctcc aaaaatcttg agaataaaca ctggcaaaat attcataaca
     8101 attcccacaa tcataaatat aattatatac aatttagtat ttttattaaa aaattttata
     8161 tatggcttta attcattcaa ttacaattcc tccttcttag tcatgttaac aaacatatct
     8221 tgtagcccta tgtatcctac atcaatattt gaatttttaa gtattttttc atcattttca
     8281 ctaaaatcac catagtaaaa ataagcaact gcatttccaa atgccttttt aggttttata
     8341 ttttttatac attctaattt ttctaactct tctttatttc cagtaatata atgagctttt
     8401 tgttttatga catcaatttc ttcatctata ataatttttc cctcatttaa tattattaca
     8461 tgctccagta aatcatctat ttcatcaatc aaatgagttg atattattat tgtccttgga
     8521 ttttttatat aactatctaa aattatgtta taaaactctt gtctatttac tgcatccaaa
     8581 ccaattgttg gctcatcaaa tatggtgata gctgaattag aacaaatacc tattatattt
     8641 gaaagtatag ttttcatacc tcttgacagt tgtttatact tttttttagt atttaaatca
     8701 aaatatttgc ataattcttt ttctaagcct ttatcataac ttttataaaa actagaagaa
     8761 aattcaaata tctgacctac tttaaaatct ggactataaa attctttttc cctgacaata
     8821 caaacttctt ccaaaacttt tatatcttct tttgggttct ttcctaatat ttctatctca
     8881 ccttcatttt gaattaactg atttaccatt atatttaata atgttgtttt tccagctcca
     8941 ttcttaccta ataaaccata tatcttattt ttttcaaagt ttagagacat attattaaaa
     9001 atattttgct tcttaaaact gtgagataaa ttttctatct taatagaact actcatatat
     9061 taatcctcct tgaaattaat tataatatca accaattctt ctctatttat ttctaatttg
     9121 ttagcttctt gaagtaggtt ttttacaaaa ttatgtttaa aattttcttt tctagcttct
     9181 tttataatag tttttgcacc atctgataca aacatcccta tccccctctt cttatataaa
     9241 atatttttat caaccaatat atttatacct tttaatactg tagctggatt aattttataa
     9301 agctttgaaa gctctgttgt tgatggaacc tgttcttctt cttttattcc atttgatagt
     9361 atctcatctt caattgctcg tgctatctga ataaatatag gttcttcatt atttaatact
     9421 aatttcatag gttcctcctt tctccattag ttagttactc aactaattaa ctatataact
     9481 tataatatct tgaaaaatat tttctgtcaa tactaaataa aaaacagagg gaaaattccc
     9541 tctgtttttt gttctttata tttataatta aattttgatt tttgtaatcc tatcccataa
     9601 gttaaaaact ttaataatct gttgtatctt cattttttaa tattttacat attgagctag
     9661 ttcctaacct ttctgctcct gcctctataa atttttctgc atcagaaatt gatttaactc
     9721 cccctgcagc ttttattttt acattttttc caacatgctc tttcataagc tttatatctt
     9781 ctaaagtagc tccacctgtt cccattccag tagatgtttt tataaaatct gaacctgaca
     9841 ttgttactat ttcacacatt tttatttttt catcttcatc taaataacat gtctctataa
     9901 tgacttttaa tactttatct cctatagctt tttttatttc ttttatttct tttcctatat
     9961 tatcataatc tttatttttt atatctgaaa tgtttacaac catatcaact tcatctgctc
    10021 cattttcaat agcatctttt gcttcaaata cttttgtagc agtagtttga tatcccaaag
    10081 ggaaccctat cactgtacat atctttattt taccttttag atattctgat gctcttttca
    10141 catatgatgg tggtatgcaa actgatgcta cactcatatc tacagcttca tcacataaaa
    10201 ttttaatatc ttcccatgta gttgtagctt ttaaaatagt atggtctact gtttttaata
    10261 tatgtttcat aaaatcctcc taaatttata attaatataa ttagattact tttttaaatg
    10321 caattttctc tttgcaatat tcagtatcat atcaacctct tcaactttaa aaatagtcat
    10381 tagtagtgta taaattccac ctccaactat aactgaaata gctagtgaga caaactcatt
    10441 aaatgttcct actcccaaaa ttccaaatat aaatttatac gtaaagtatg ataaaacacc
    10501 cataattaaa gatgcaacta ccgatttcaa agttgctttt attattttat cttgtccaaa
    10561 atatccaact tttctcttta ggttcaagaa gagtaacaat atacatgcaa ttgatgatga
    10621 actagttgca agagcaagac caccatgagc cattggtttt attaaaacta agtccaatac
    10681 tatatttacc cctacagata taatcccatt taccataggt gtttttgtgt cttgtaatga
    10741 ataaaaaacc tttcccaaaa tatctcttaa gccaaatgca gtcataccaa ctgcataaaa
    10801 aattaaggca gttgctgtca tttgggttgc cctcgcatca aaagcacctc tctcaaaaat
    10861 gattctaaca acaggagttg caaaaaaaat agaagcaact gaaataggaa tcatagaaat
    10921 tattatcata tttatactag atttaactga actagtaaac ttcttttgat tattttctgc
    10981 acttaactta gataacatag gatacataac agatactatt gaagctgtaa atgtccctgt
    11041 aacaaaacca tttaatctat ctgcataagt aagtgctgga atgcttcctt taactaatgt
    11101 tgaggctaaa gttgtatcta ccaatgagtt cacttgatta acagcaacac ctataaatac
    11161 tggtgaaagt aatgctagta atttaataag gctatcatct ttaaagttta agtagtataa
    11221 gtatttgtag ttcgtttttt taacaaaaaa catgtaaaag agcagttgaa ctaccatagc
    11281 aaccacagca ccaattggaa gtatgtatgg tccaaatatg gtacttaaca ttattgaaat
    11341 aattattact atattatagg gtatacttcc aaatccaact acaataaagt tctctttaat
    11401 ttgtaaaaat gctgacataa cacttgtaat acctatgaat attattcctg ttctgaacct
    11461 cccatgacta aagtcacagg gttcctgctt ca
//
LOCUS       DAMJWS010000108        11194 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMJWS010000108 DAMJWS010000000
VERSION     DAMJWS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.113"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(52..684)
                     /locus_tag="PC399_003290"
     CDS             complement(52..684)
                     /locus_tag="PC399_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2594120.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(756..1109)
                     /locus_tag="PC399_003291"
     CDS             complement(756..1109)
                     /locus_tag="PC399_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2594121.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1187..1390)
                     /locus_tag="PC399_003292"
     CDS             complement(1187..1390)
                     /locus_tag="PC399_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2594122.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            complement(1780..2010)
                     /locus_tag="PC399_003293"
     CDS             complement(1780..2010)
                     /locus_tag="PC399_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2594123.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(2012..2419)
                     /locus_tag="PC399_003294"
     CDS             complement(2012..2419)
                     /locus_tag="PC399_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2594124.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            complement(2779..3228)
                     /locus_tag="PC399_003295"
     CDS             complement(2779..3228)
                     /locus_tag="PC399_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2594125.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(3242..4168)
                     /locus_tag="PC399_003296"
     CDS             complement(3242..4168)
                     /locus_tag="PC399_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594126.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4293..4697)
                     /locus_tag="PC399_003297"
                     /pseudo
     CDS             complement(4293..4697)
                     /locus_tag="PC399_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            complement(4847..5467)
                     /locus_tag="PC399_003298"
     CDS             complement(4847..5467)
                     /locus_tag="PC399_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2594127.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5566..6834)
                     /locus_tag="PC399_003299"
                     /pseudo
     CDS             complement(5566..6834)
                     /locus_tag="PC399_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6926..7741
                     /locus_tag="PC399_003300"
     CDS             6926..7741
                     /locus_tag="PC399_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDF2594128.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSSRAEMERL"
     gene            8413..9180
                     /locus_tag="PC399_003301"
     CDS             8413..9180
                     /locus_tag="PC399_003301"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2594129.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9508..10185)
                     /locus_tag="PC399_003302"
     CDS             complement(9508..10185)
                     /locus_tag="PC399_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594130.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10335..10652)
                     /locus_tag="PC399_003303"
     CDS             complement(10335..10652)
                     /locus_tag="PC399_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594131.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10674..11012)
                     /locus_tag="PC399_003304"
     CDS             complement(10674..11012)
                     /locus_tag="PC399_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594132.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3705 a         1778 c         1617 g         4094 t
ORIGIN      
        1 cttataaaat gattattaaa gtaaagttta atataaataa attataaaat attaaactaa
       61 tttatcaata tttcctgatt gaatatgctt aatgtttgat tgaatttttt tatatggcca
      121 atcccaccat ttaatttcta ataattttga aattgtatca ttattaaatc tcttttttat
      181 aatttttgca ggtactcctc caacaataga atacggtgga acgtcattag taactactgc
      241 tcttgttcca ataattgcac catcaccaat atttacacct gacataatta tagaatcata
      301 acctatccac acatcatttc caattataat gttaccctta ttgtcccaag cgtccgttat
      361 atggttgata tcgagtttcc attcttcata aaagatggga aaagtatatg tggattttga
      421 tttcattgag tgatttccac tagtcattag aaattttgca ttacatgcta ttgagcaaaa
      481 cttaccaatt attaatttat cattgtttat agggtaatga tacaatacat tgtttttctc
      541 aaaatctctt gggtcattat aaaagtcatt atatattgtg taatctccaa cttctatatt
      601 ttcccttgtt attacatttt taagatatac agtttgataa tcattacttc ttggatatac
      661 cttatttgaa cttggaatag tcatatttaa atctctcctt taatatatat tatgtttttg
      721 actattccac tactacaata catctcttca cagaatcact ccttgttaag aatcttcata
      781 agataatggc gtcctttttt agtgttaagt cggatattct ttcttagtcc attcttttta
      841 gcgtataact gacaaactgt cattttctta ccaatagtgt caatgctatc atcaaggtca
      901 ttttgtattg ctttttcctt atcccttaat gatatatcat cacgttttcc ctttgggggt
      961 ttatcagtag gtacaagttt ccaagagtaa acaaattgtg gtttaccaaa tgaatataca
     1021 tacttatatg cgtatcttcc atcttttctc tggctctctc cagttcttaa aattctatct
     1081 ttattatctc gtcttttttc agttgacatg ataagaagct cctctcaata accgaattga
     1141 gcttgatacg tcatatgaat attataacat ataaggctca atttcattag ataaagtcaa
     1201 gtgtatcaac gattttctca aattgttttc tctttatttg aatgcgattg ctattcataa
     1261 tgacccagcc agaatcaagg ttttcatcag caagtttacg taattttttc tctccaatac
     1321 gaaagtattt tgacgcttct tcaatcgtca aggtatattt ttcccaaata ggtacatcag
     1381 tattgttcat agaatgtatc actcctttct atgttcgtag ttcagaagta gatagatggc
     1441 tttggtaagc tccattggta ttttaaccaa tcccattctt atgggcagaa ccgtgtcata
     1501 caggtgtatc attatactgt ggtacaggtt acggcaaaac aaccgttcca aaggttattc
     1561 acaaatcact atatagtagc tctcttgatt aaggtcatgg cgtatagtat ttgttggcta
     1621 tctgtatcac aaacgtatct atctacttta ttcagttgtc aaagaacagt cggcattgat
     1681 ttgtctataa aaaatcacaa tagcttatgg gcttttatag acaaataatt agtgattctc
     1741 catgtaccaa atagcttgat acatggagag aggacgagat taagacaaat taaatctcaa
     1801 aatagcagtg attagttttg ttcctagcat tcgctttgtt tcttcttcta catgaatata
     1861 gggatttccg tattcatcaa ataaagtttt ctgtgaaaga cgaatgatat agtgttcaaa
     1921 atgtttaagt attccgttta ttgcatttac atcgccatgt acagcaagta aaataattgt
     1981 aaaaggtaat acttctttag taggtttcat ttcaatgttc ctccatgagt tctttcaact
     2041 cttttagtgc atttgttttg tgataatgta tggtattttc tgctaaatcc atcaatgttg
     2101 ctatgtcagc atttgtcatg ttgagaaaga atgaaagtag aattatatct tgttttcttt
     2161 ttgtaagttt tccgattgct tgtgccatta atacatcttc aattcgtatg ttgtagtcgt
     2221 aagaaaccat acagatgtag ttagaatcat aatcatctaa aatactcaat ttacttaatt
     2281 ctgcaagtga caaagattcg agagatacca aataatcatt tctgcgttta ttttcgtcat
     2341 aaatatctct agcatagtta cgcaaaactg ttttacagaa actgtcgaat tgatgttcaa
     2401 ttgtcttttc atcaaccata agatttcacc ctctttccat cgcaaggagt taaataacct
     2461 tgcttactcc tatcacggtg ggagggcgtt aaatcttcga taaatttcaa aaaaaagaga
     2521 aaaaaataca cactctgcct atagggcaaa aatgtgtatt ctttaaaacg tatttaattt
     2581 atattggttg taagccacca ccatattgat gtatggaaat atgcattggc tagcttttca
     2641 taaaaataca aagatgcata agaaattaat agaggggaaa aacttattct gttattaaaa
     2701 agtaggttaa ttctaataaa tgctattgcg gtcgaattaa actatataca tggcaatcaa
     2761 tcatgtttct attctttatt atgaattgtg acagtattct tcaatcttaa aaccacattt
     2821 ttccaatact ttacttgacc ctctattgta ggaatataca ttagcatgaa ttttgttaag
     2881 gttgtatttt tcaaaggcaa ttttgcatat ttcttttact gcatttgttg ctatcccttt
     2941 gccccaatag ttcctagcaa gccagtaacc taattcagca tttttattaa tagaggtatc
     3001 ttcttcaaat gttaggctga tacttccaat agccctttct tgataaaaaa tagcatagct
     3061 aagttgttta acatctggag ttgccaaaca aaaactaaca aaatctctag cgttttgctc
     3121 cctataaggg tgggggaagt tgtcacgaag attctttgca atttctttat cattcgcaaa
     3181 ctcaactaaa tcggtaatat aagcataatt ccactctata agttccattt ttcttctcct
     3241 tttatgaaac agctacaaga aatctttcta agccaatacc acttatcaaa cagcgttctt
     3301 ttttatttcc aagcatttga taagtccagt caacaaatcc accgtcaact atttcaacgg
     3361 tttcatttcc ttgtttgacc gataatttga aattgatacc tttataataa ttattatcaa
     3421 catcatcatt atgaattgat aactctatat taggaaaaat aagtttgata gtttcaacca
     3481 ttctgtcgaa aaatccgtca ttatctttgt atccgtttct tttgcgtaga ataatagaga
     3541 aacgaacatt ttctatttca cttaatatat ctttataata gtttaagtgc ttttctaaca
     3601 acattttttc acaagtgtaa gaccctgtat ctataccaga tgaaaccata caatataagc
     3661 caaaatgtga atataaacct ttacctacaa aattttgtgc tcttattacc ctagcagtag
     3721 tagccatgtg gataggtatg gtattatttt cttcttttct ttttaattta tcagcaataa
     3781 ttattgccaa catatttgag gggtctgcaa gtgtttctgt ccctcgtaga gcacttacta
     3841 cattatattg atctacggag ccaaaaacgc tacagctacc taatggagca gagggagaaa
     3901 gcataatcgt cttgatatcc atattgaatg cttttctcaa taattttgac tccaaagcat
     3961 gaatttcttc tggatctata ccgctaggga tagtaaatct atttagttgg tatgacttta
     4021 gaatatcagt tgcagttaaa gtatttgctt gtctatcaaa cacttctaat agaagtgagt
     4081 tcaaatctga ttttgataga gctgataact tatcaactat attcttatcc ccaattttat
     4141 ttgtaattct acttaaaata tcactcatag ttatcctcca tactgttact ttcaaaataa
     4201 ttatagaatg gttacaacaa ctaatctagg tcatttgacc aaaaatgaaa aaacactgaa
     4261 tatttactat tcaatatttt tttactttgg gtttatttga ttaaatcttg aaaatactgt
     4321 aatgcaaccc tttcagcttc aacctttcgg acaataggac tttgtgataa ttcctctctt
     4381 aactcaagat acaggttgat agtagcctgc aatgcattaa cacagctttt atattcatgg
     4441 ttagcaaccg tcttttctaa tgcttcaata tattggggtg cgtccaattc aatttttcta
     4501 actccacgag gtaatttacc taagagaagc tgtattaatg gggcaagtac tgattgtctt
     4561 aaaaaagata aaaaatcaat tgtttcaaat agttctctac gtccaatctt agtgcaagcg
     4621 taatgaaccc aaatccaaaa tctatcttca atccactgta agtctggagt tgggaagtat
     4681 gcttcttttt tagtataaca cctctcactt tcttttatta gctatcaatc caacataatt
     4741 atgcatagga gtattatata atgaaaaaat aaaaaaaaat aggtcacttg acccgaaata
     4801 aaaaagcatt gaataattat tattcaatac ttttttgaaa ttatacttat gttgtaggaa
     4861 gattctttaa tacctctcgc tgattaatta cactaaatct tttgttttca taagcaatta
     4921 gatgttcttg tacacattca tttatagcac gattaatgct tctaattgga gcgtttactt
     4981 cgctagcaag ctgttttttg gtaagcagat ttaattcact tctataatag tgtaaggata
     5041 tacacctcag aagtcgttct ttaacactta aagatgataa ttcttcaatt aattcagcat
     5101 ttacaactaa tttactagat atttctcgaa tcagaaattt tgtagcttca aaatctcccc
     5161 ttaaccagtt taaaaaatca tctctatgaa gtttatatac attacaatct gtgaaagcga
     5221 caatttcaac gggttttttc ccatcattaa attgttccat ttctccaaaa aaactaccac
     5281 ttctataaag atgaatagtt gagatgtttc ctttcacatt taagatgaat gcttctgcaa
     5341 taccgtcaat cataagaatt acatattcat tttcttgctc agcaaaaaga atagtctctt
     5401 tctttttata cgttttttta acaagcttct ttttaatatc ttcattcata aaatcaatta
     5461 aatgcatatt catccctcct taccaaatct ttgattaaat tatatcatct ttcaattagt
     5521 taggtaagat agtaaaaata tatttacata gaaactattc cattattatt ttccagttgt
     5581 catttttctg caaagtaagc tcaaactgag aaagctgttg acaatagaac tagtaggaat
     5641 atatgagcga accatttcag tattcaactg ttgtaggttt tcagtcagat aattcttcaa
     5701 acgttcaatt cgtttgtcct gtgaatcttg cgatgctcta accagtttca tagatagcgt
     5761 tttaccttgt gccacctgca aaaaagacca tgcttcgtca aggtcaacta tcttgaagct
     5821 agagcggtca gtatgaataa agtctaatgc aaaggtacta ataacaatca acatagcaac
     5881 actaagaagt tctattgttg tatattcttc aaagctagtg ttggcatctg gcaatacgag
     5941 gtcagcaact tgaataatat tcagctgttt ctccaagctg atagactgct tcacatttcc
     6001 gtcaccaaac agtagatgtg caaaatcata gtctacaaaa ctttcgatat ggtcggctat
     6061 gctgttactg atagaagtat cttcttttcg taattcatca atgacaagta ataaccctcg
     6121 ctgttcactt tgggttacat tgcggattgc ttttctaagc actgggaact tatcactgtc
     6181 acgactggag ataccagcaa ggaatgttag gatatcaatc gctaaacttt cagaatcatt
     6241 tttacggctc aaaatgacgt aagggtcaag caatcctttg ttttcatcat tactggttaa
     6301 attgacaatg tttatctcat gggctatttc tggtagcgtt tctttccatt tgccgcgttc
     6361 tgctttaggg tcaactatga ccgcctgtcc gccgaatagt accgcatagt aaacaagcat
     6421 attgttactg aataatttac caccacccaa tgaaccgata aacgcagacg ctaacacatt
     6481 ggtaatagaa ccctttaccc attgactagc aagggcaggt tttagataga cattctttcc
     6541 agtgtccaga ttgtagccaa gatagatatc ctcatgttcg ccaagggttt gtgtcgctcc
     6601 aaaaccaagt ccagccaaaa aatccgaagt gacatattga atataatcat tgatgtaacg
     6661 tttgctgaca ggaataaatt cgccatgcaa tcccaacata tccccaaagg gacgcacgag
     6721 cttgagtgat aagtcgtcgt aaaagtcttt gacttcatca caacgccttt ttagctcgtc
     6781 aagatccttt gtagatacac ggacaacata gctaagttta tacatagatt ctttacagtt
     6841 aaaagtctgt ttttaaaaaa atgtataaat tataaatcaa aatatagata ctatcaatga
     6901 cccactaggt gcaaaaagca ttgatatggt taaattatca gacgctaaaa gctgggctat
     6961 ccgaatgagt gaagatgggt gtgcttataa atccattgac aattataagc gctcactgaa
     7021 agcgtcgttt ttcatggcaa ttcaagacga ctgtattaga aagaatccat ttaattttca
     7081 gttaagtgat gttttggaag atacaacaga accaaaaatc gctctaacac cagaacagga
     7141 aatgggctta ttatccttta tggagagcga taagacttat tgtaaatatt atgatgatgt
     7201 tgttatcctc ttagaaacag gactacgcat ttctgaatta tgcggtttga ctttagaatt
     7261 ggatatgaaa aataaagcca ttatggtaga ccatcaattg ttgagtgata ctaagattgg
     7321 ctattatgta gaagttccaa agaccacaca gggtaagcga gaaattccaa tgactgaaag
     7381 agcttatcaa tcatttgaac gtatcgtgga acggcgtaag aacgctaaag cagaaccaat
     7441 cattgttgat ggttaccaga acttcctatt tcttaaacaa gatggattac caaaacaagc
     7501 aactcattat caaagcatgt taagagcgtt agtcaagaag tataataaga cccatgagga
     7561 caagctacca aagattacgc cacatacctt taggcataca ctctgtacaa atatggctaa
     7621 caaaggaatg agtcctaata tattgcaata tattatgggg cataaaaaca ttactatgac
     7681 gcttggctat tatgcacatg gttcatttag ttcttcgaga gctgaaatgg aacggttata
     7741 atcctagtag tatgactaga gtttactact tatttactac ttttgatggc attcttatac
     7801 ggttaaatgc caacttatgc aaagtatctt ccaaaaagaa aatgccgata ctccttgtag
     7861 atacaggata tatcgacatt taccaactta tgaaaagata atcaaattta atataagttt
     7921 ttcaaataaa aagataccaa attctttttc gagaatctat ataacattac ttaagcttgg
     7981 ttggggtata aacaattgag tagctacttt tgtatggtgt tcaactttag tcaaaactct
     8041 taagtaatgc aaatgattta gattcattaa tattcaatct cttcataagt agtgaaattc
     8101 gagttatttc tgcataatta tactatgatt tatagattaa ttctattagc atccagaata
     8161 atattgttta tatgagtaaa atttaaagaa tattgtgaaa attataggat gttaaaataa
     8221 atttaggtaa aaaagaagtg tttgtatatt atagagtacc ttttctaaaa tatgtgggat
     8281 tgttttaaga attagaataa aaattatttt taaattagta tttttataat gtatagtatt
     8341 tttaatgtat aattaaattg tgcagaaaat agaatttaaa gtcctataga atttctgatt
     8401 atggaggaga atatgaaaag aaaagttcaa gtaaaaaata ttactattgg agaaggtaga
     8461 cctaaaattt gtgtaccaat tattgggaaa aataaaaaag atattataaa agaggctaaa
     8521 gagttaaaag atgcatgttt ggatattata gagtggcgag ttgatttttt tgagaatgta
     8581 gaaaatatta aagaagttaa ggaagttctc tatgaattaa gaagttatat acatgatatc
     8641 cctttattat tcacatttag aagtgtagta gaagggggag aaaaattaat atcaagagat
     8701 tattatacta ctttgaataa agaaatttct aatacaggtt tagtagattt aatagatgta
     8761 gagttattta tgggagatga ggtcatagat gaagtagtta attttgctca taaaaaagaa
     8821 gtgaaagtaa ttatatctaa tcatgatttt aataaaactc caaaaaaaga agagattgta
     8881 tctcgtttat gtagaatgca ggaattgggc gcagatttac ccaaaatagc tgttatgccg
     8941 caaaatgaaa aagatgtgtt agtgctactt gaagctacaa atgaaatgtt taaaatttat
     9001 gcagatagac caataataac aatgtcaatg tcaggaatgg gtgttataag tagattgtgt
     9061 ggtgaaatat ttggttctgc attgactttt ggagctgcaa aaagtgtttc agcaccaggg
     9121 caaatatcat ttaaagagtt aaattcagta cttaatctat tacataagag tattaattaa
     9181 attaattata tagaaatatt agacggtatt tttgaagata taaattaaat ataaaaaact
     9241 tttcagatag taaaatttaa attgggcaat tatatctgaa aagcaactat ttagtgtgta
     9301 ttttgaaagt gatatagaaa atcttataaa tcaactatgg catatttaaa atatgccatt
     9361 aaaacctact agacattaga ttatagatgg tatactagca tttctattat ttatagatag
     9421 taagttttca taataactgt tttatctaaa gtagaagata tatttgtatt atgtatattt
     9481 gtcattattt ttttgtaatt atgtatatta atggttatat atgtaatata atattatcac
     9541 tctatttttt gtatattgta cattacaaca tttatatcta tcatcatcaa ttacagtaga
     9601 atgtatggga tacttatttt cacttaacca atatttttgc atattctttg tccaagactt
     9661 tggaaacttc aatactttct tgtttattat aacatcctca tcttcaaaaa tattaacttc
     9721 tctttctatt tttatttttc tgttgtatat cttattgata gattctttgt cgagttttat
     9781 gtctctatac atatcagggc aatagcaaca aacataattt cttttttttc gacgtttaaa
     9841 tttcacaact ttctctatgc taatttctaa tttttttgta ccatctttta taaatttaat
     9901 atcttctatg ttctcgcttg aaaaaatttc tgtcaatctt aaagtatcta cagtttctct
     9961 aattatttgc tttttataag aagagaatcc atatccaaaa gaaaggttta atttattagg
    10021 gatgtagcca tttttatatt taaacatctt aattttttcc aattcagcat tatctttaaa
    10081 tttcaacttt tcatacctaa aagttacttt gctaaaaaga gtatattcta catcaatata
    10141 ttcttttgca atcttaaatt tatcgctacc ttcaatcaca atcatagcct atctccaatc
    10201 gttttatatt aaaataaggc catagaacat ggtctaatag tactcattag cactataaga
    10261 caatgttaac acaaaaagac aaaaaaaggt tatttatttc aaataacctt tttttttata
    10321 taaaatataa agaattatat tttattcttt tttatgatgt ctataatctg tgatatcaga
    10381 tttagttctc ttgaatttaa gtcttttaac tcttcattta tatcattttc taaatatgtt
    10441 tttccagtgg aagtaatatc ttttataaga acatctgcag aaatatttaa tatatttaca
    10501 aattttatga atgttttaag acttggattc tttttaccac tttcgatttg agttatatac
    10561 caaggcgata ctccaaccat atttgcaaat tcttttgaag ttaaatttgc ttttttccgt
    10621 tcttctttaa gtcttttacc tatataattc attattgtta ctatttccct cacttatatt
    10681 tctttgtatt taatgatgca ctttattaac tctagcattg caatttgttc tttttcagtt
    10741 aatttattaa tcatttcata acattctaaa ggtacagagt tatcaacgcc ttctgttgta
    10801 ataatatttg caaataacaa ttcaagtggt atgtctaaag cagtagcaat tttcattata
    10861 atattgatag atgtatttgt ctcagctctt tcaatctgac caatatatgt tggatgtaaa
    10921 cctgcctttt ctgctagaaa ctcttgacta tatcccaatt ttgttctata gacttttatt
    10981 ctatctccta ttatttttcc tatatttgcc aattattatc cctccacttt agattatatt
    11041 catagtttaa gttgttttat atagactatt tatacttttt tagtctaaat atagactata
    11101 tatgttttta tactgaaata agtgctattt atttttgtat agtctgtatc tacaataaac
    11161 aaatatggat aaatcatatt tttaattaga gaaa
//
LOCUS       DAMJWS010000109        11109 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMJWS010000109 DAMJWS010000000
VERSION     DAMJWS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.114"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            16..1497
                     /locus_tag="PC399_003305"
     CDS             16..1497
                     /locus_tag="PC399_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2594133.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVYDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1530..4967
                     /locus_tag="PC399_003306"
     CDS             1530..4967
                     /locus_tag="PC399_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2594134.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLITYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVELGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            4985..5731
                     /locus_tag="PC399_003307"
     CDS             4985..5731
                     /locus_tag="PC399_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF2594135.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5680..5988)
                     /locus_tag="PC399_003308"
     CDS             complement(5680..5988)
                     /locus_tag="PC399_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2594136.1"
                     /translation="MNFLKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIEKYKT
                     SYKQLLVNPNIEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            6791..10924
                     /locus_tag="PC399_003309"
     CDS             6791..10924
                     /locus_tag="PC399_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2594137.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISPPIGST
                     NLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDADNGTF
                     GFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSNVIGA
                     WWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVTGYPP
                     PQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFSEGES
                     AIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTNVFFR
                     DLIPNGLEFIPGTVTVDGVSVPGVDPNTGFSLPDIGGGTGVEVTFDVVVESIPNPNPT
                     NNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEELIYTI
                     KVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETRVIII
                     RGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNSAVPG
                     TLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGTLNSG
                     ISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISIIKVAD
                     EDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWNGSLN
                     IGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSADIEV
                     IKTSNMDTAVPEETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVTFQPW
                     NGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDETDIS
                     PLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYSIDNG
                     VTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNNNTST
                     SEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPASILSP
                     EYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPN
                     PDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTLIDNV
                     SDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNIAEVT
                     STTPDSNLNNNISRVQVEIKQFCCNDCGCKDCCCNCCCNDCGCKNCCCNCCKNDFWC"
BASE COUNT         4480 a         1325 c         1760 g         3544 t
ORIGIN      
        1 gtgaggtgta gctatttgaa aaaaaataga ttaatagcaa agaatatatc aatagcattt
       61 atagtactat tcttttttag tgtatttaca ttcttttatg taggtaatat taatagagtg
      121 ttagaatatg aaacaaatga cataataaca gttacaattg ctggatggat tatactttca
      181 tttttatttt taggtattat aatttacata ttatattcaa aagcaaatag tcaaaaaact
      241 atagaaaaag tagcatatac ggattttgta acaggttatt ccaactggag aaagtttgaa
      301 ttagatgtta caaatttatt aaagaaaaca tcacaaaata ataaatatgc aatggtcata
      361 ttcgatattg ataaatttaa agcaattaat gatatatatg gccataaaaa gggaaattta
      421 atattaaaag atattgcaga tactttaaat gaattgactg atatcaatga aacttttgct
      481 cgtgtaagtg ctgataattt caatatatta ttaacatata acaaaaaaga ggatataata
      541 aatattatca aaaagattat ggcaaataat gaacttgtaa acttatcttt tggaatttat
      601 gaaatcaaag ataaggattt atctgtaagt gtatatagtg atagagcttc acttgcaaaa
      661 tcatcaataa aaaataatag tgatgtaaat tttgcatttt ttaatgacaa attaagagag
      721 aaattattat ttgaagataa gatagaaaaa gaaatggaat atgctcttga aagtgggcaa
      781 ttcgtaatgt atttacaacc aaaatacaat attaaattag ataaattttg tggaagtgaa
      841 gccctagtca gatggcaata tacagaaaaa gaggtcatat atccaggtga ttttattcct
      901 atatttgaaa aaaatggatt tattagaaag attgatatgt acatattaga acaagcttgt
      961 aaagaaatcc ggtctttatt tgataaagga atttcacctt tacctatatc tgtaaacttc
     1021 tcaagagttg atttttttaa aaaagatttt attgaaaaca tagtaaatat atgtgataga
     1081 tataaaatac catatagttt aatagaaatt gaaattactg aatctagtat gtttggagat
     1141 actgatactc tatttaatgt tagtagaaac ctacaggata ttggctttat agttgcaatg
     1201 gatgattttg gctctggata ttcttctgta aatatgctta aaaatatacc attaaatgta
     1261 ataaaattag atagaggatt ttttgtttat gataaagatg ttgataagag tcaaattgtt
     1321 ataaaaagca tagtttctct cattaagcaa ttaggaataa gggttgttgc tgaaggaatt
     1381 gaaacaagaa gtcaaataga aatgttaaaa aaagcaaatt gtgatattgt tcaaggatat
     1441 tatttttcaa aaccattacc aattaaagaa tttgaaaaat tagtctataa aatatagtca
     1501 gcgagtctta aatgggaggt ggtgattaag tgataagagc aaaatcgtta aaaaatgtac
     1561 tgagtattgt tttaatattt ttatttacaa taatttcttt ttctgatttt gcatttaaaa
     1621 ctaatgtcca ggaagaagaa aatagtaaaa ttaatttaag agaaatatcc ctacaaagtt
     1681 ccaatgctat aaaaataaaa ttagatagtt ctctatcctt tattaaaagt gtggcaaata
     1741 tggtttctta ttttaatact atagataact caaaaatagt tccattatta gctaatatat
     1801 caaaagaatc tgactttcaa agaatctcta ttgtatctcc tgatggaata agtcatacca
     1861 gtgatggcaa aattatcaat gtgtcaaagt atcagtattt taagaaagct attaaaggag
     1921 aaagtaatat atctgaagtt gtgaaatctc caattgatgg aaaagatact tttgttatag
     1981 ctactcctgt atttaataag aataacaaaa taattggtgt tttatacggt tcttactaca
     2041 atgataaatt agctgaattt atagatataa caagttttaa tagtgaaggg tacatagata
     2101 tatttgaatc tagtggtcaa tttgttttaa aatcttcaca tacaaacaaa ctgattaagt
     2161 caagtagtaa catatatgat atatttaaaa aagcttcttt tgatgatggt tatagctata
     2221 aaaaaatata tgaagataca aaaaataaca agagtggctt tacctcatat gaatacaaag
     2281 gagaaaatcg ttatgctact tatacaccca ttggtgtaaa tgactggtat gtaatttctg
     2341 tgataccaca atatactgta agaagaaaca ttgataaaat aaatcaatta gcattttctc
     2401 ttattttcaa ggtattctta ttgtttatta tgcttataac atatattatt tataaaaaca
     2461 ataaatctca gaaagatata ctaaaagcta acaaagaaat ctcatctatt acaaatagca
     2521 ttcctggagg agtacaaaag tgtaccctgg gtgaaacatg caatattaat tttttaagtg
     2581 atggatttat agagttgttt ggttatacaa gagaagaaat aaaaacattg tttaataatg
     2641 atttttatag tttaatacat aaagatgatg ttgaaagaat aaaaaaagaa atttttaaac
     2701 aactttacaa aaataaaact gttgaattag aatataggat tataacaaaa aatggtaata
     2761 cagtatgggt tttagataaa ggggagctta taacagaacc tgatggaaaa gaatattttt
     2821 gttgtgtaac tgtagatatt actagaacaa aacaattaga acaagagatg gaacaaaaaa
     2881 atctagaact tgaaatgata tcagaaaatg ttttaggtgg tataattata acagaattta
     2941 atgatgattt tactataaaa tacgctaact ctggttatct ttcaatgatt ggatatacac
     3001 gtgaacaatt agaaaatgaa cttaaataca aagcttgtaa tcttgttttt tatgaagata
     3061 gaaagaaggc tcttgaaagt atacaacatc aattaaacac aaaaggaata gctgtatatc
     3121 aacatagaat aagaagaaga gatggtaata ttatttgggt ttctataaaa ggaaggctta
     3181 ttgttgatga tgatggtatt gaaaaaggta tttggattct aaatgatata acagatttaa
     3241 aaaatgcaga acaaaaactt aaaataaatg aagaaagatt tagaattgca ttaaagcaaa
     3301 catcaaacac tatatttgag tatgatataa aaacaaagtc acttttaaat actagtaaag
     3361 agcttgataa atataatatt tataaaatta ctaaaaatgt tccatattct ctagtagaat
     3421 taggagttat tcataaagat tttagtgatg attttataaa tatgtatgaa aatgtaataa
     3481 atggagcaaa aaaagcaagt tgtgtagtta agatgaaact tccagatgaa aattatatat
     3541 ggaataaagt taccttgact acgatttttg atgaagatgg taatcctgta agagcaattg
     3601 gtatacagga agatataagc aaacaaaagg aagcagagat aagatacaat caagaaggta
     3661 agtatcattc agctatattg tcggaagcaa tgataactta tgaaataaat gtatcaaagg
     3721 atacttttat caaaggaaat gaaagttggc aaaaaccatt tggaattaag cctgacaata
     3781 actattctca aatggtaaaa actatagcaa atagtattgt acataaagac aataaagaaa
     3841 catttttaaa tacattttta tgtgaaaact tattggatgc atataaaaat aatgtgactg
     3901 aaataaaatt agaatatcga agaatgaatc aagaaaataa aatgatttgg gtcatttgta
     3961 caatgcactt aataaaagat tctattacga acgatataaa agcacttgct tatgttaaag
     4021 atattgacaa gcaaaaaaat gaagaaatta gattaaaatt aaaagctgaa agagatactc
     4081 ttacaggatt atataataaa ggtactacag aaaaattgat taatgatttc ttgaactctg
     4141 aggaagcaag cttaggaatg catgcatttt taattgttga tatagataat tttaaatctg
     4201 tgaatgataa tttaggacat gtatttggag ataaagtatt atttgaagtt tctaaaaacc
     4261 taaaacctat atttagaaaa gatgatatag taggtagaat tggtggagat gaatttatag
     4321 tattcttaaa aaatattcag tcttttgaga atatcaaaaa gaaagcagaa aaaatatgcg
     4381 atatatttag aaattcttat acagaaaata aaaagacata taaaatatct ggaacagttg
     4441 gaatttcatt tgtaccagaa catggaactg tatttgaaga actttataaa aaagcagaca
     4501 tagcacttta ttatgcaaag agcaaaggaa aagataagta tgcactttat aatgattgta
     4561 tctaccaaaa tgaacttgaa aataatataa gtgataataa tataaagtta gcagataaat
     4621 gtgagaaatc caaaccagta cttaaagaaa ttcttgattc tattgataat tatatctata
     4681 tagtgaatcc aaatacttat gaacttcttt ttgtcaataa taaggtgtct cgtttggatt
     4741 tagagataaa attaggagaa acctgttata aatcactatt aaaatctgat agaccatgta
     4801 ataattgccc tcttaacgga ttagaagaaa ataagcattc aaaattcaaa atagagacaa
     4861 atacatttgg taaaaatatc agtatagcaa gttggattaa ttgcatagat ggagaataca
     4921 actgtcttat agagtgtata gattcttctt tatacaaaaa taaataaaaa aggactatga
     4981 agatatgtta aatagtataa ctttgatact tggtataata tttacaattt actattttta
     5041 tctcaaaata gtttttggag gaatttcatt tagtgagatt ttcttagttg taggaattgt
     5101 actaattatc tatcaaatat ttaagaaaaa aattaaagaa aagaaggttt tttatagggt
     5161 gttaaaaata ataatcagca tttttttaat agtatttgta gttactgaga gtctaattat
     5221 tttttatccc aaaaatagtt tagaatcaaa aagtgattat ctcttaattt taggagcttc
     5281 tgtaaaaaag actacaccaa gtacaaccct gaagggtagg ttagacactg cattgaaata
     5341 cctaaaaatt aatgataatt gttatgtggt agtatctggt ggtaaaggaa gtggagaaaa
     5401 aataactgag gcaaaagcta tgaaagatta tcttataaaa aatggtatag ataaaaatag
     5461 gataatagaa gaggataaat ccacgaatac ttatgagaat tttaaatatt caaaattgaa
     5521 gatagaagaa catagtcaaa gaaaactaag cgatttaaaa gtaaaaatag taacaacaga
     5581 ttttcatgtt ctcagaagta aaatattagc atatagaaat ggatataaaa atacgtcttt
     5641 ttatgctagt aaatcaaaac tttcatttgt accaacttac tatacaagag aattttttgc
     5701 attatggaaa acaatcattt ttgatagata ataaataaaa agactagata taaactcata
     5761 tctagtcttt tctattccct taattctaat ccattttcca actttacttg ttgtagaaat
     5821 ttcaatattg ggatttacaa gtaattgctt ataagaagtt ttatattttt cgattccaaa
     5881 atataatttt ccttcataat ccattataaa tccaaatggt cttactcttg gtttattatc
     5941 ttcaactgta gcaaaataga atgttgagtt ttcttttaaa aaattcatta ttttttcatg
     6001 attaaatcct ctttgtatct tatactaata taatgaatat aaataaaata ttcattatac
     6061 ttaatataaa tcgatataaa aacaattaca atattatatt gattattcga aattatatat
     6121 aaaattcaga aaaaatttta gtttacataa caaaaataaa gtcaactaaa attattgatt
     6181 ttagattaaa gtaccggatt aagcatttaa gtagatttta agcttgattt gattggaaag
     6241 gtacaaacta tcaccgaaaa aaattaaacc catttaaaaa gcaatataaa agcttgttat
     6301 ttttgagaaa aatagactct ctaaactcaa ttagattaaa aataaaatac agaaaattat
     6361 atctctaaat atcttaaata attaagttta aattttacga tattttgttt gattatttat
     6421 aatataaaag catactctta atcttcaaaa tttaaaaaga gtaaaaaata acaaaaaaat
     6481 cactgacatt tcactgacaa attgtaattt tgttattata aatacattga aaatacttga
     6541 ttattgaaaa atggcataaa ataatacatt ttatgccatt tactattatt tgatagtttt
     6601 taacttatga cataagatat tttttaacac atacaataaa atataaaaaa attgatattg
     6661 caaataaaaa aaatacatat tagatgttat agaaaaaaag cgcacttata ggcttaaaat
     6721 gtcacataaa agagttttaa agaataatat attatgaaca caaattttgt aatcacaaag
     6781 gagtgataat atggcaacag tatcaggaca aatagttttt gatagaaata gaagtgctac
     6841 cattgattct ggagactcag gaatagccaa tataccagtt gtattacaag atatagcaac
     6901 tggagttaga cttgtggttc tcacagatgc atcaggaaat tatgttttta ctaatgttcc
     6961 aaatggtaac tatagaattg tagaagcttt tggaactaca ggaggagtac caacaccagg
     7021 gaattttgca acagcgatag taggtccagt tccaattgca gaaatgccac ctattagtcc
     7081 tcctattggt tcaacaaact tagatggtac aacacctaac acactattaa taacagtagc
     7141 aggttcagat ataacaaatc aaaatatatt aaatgggcct gtgatttata cacctataga
     7201 gaatatattg gattcttgcg tatctgtatc taatataaac cttattacag atgcagataa
     7261 cggaacattc ggtttttttc caccaggtac accaagtaat acaggacctg aaatagaacc
     7321 ttacccaaat gtgacgccag attttacgta tgttgtacca gacccaacta agtttacacc
     7381 tttagatgga gaatatacag tacaaaatat tatgacaaat gcaatgagta atgttatagg
     7441 agcttggtgg cgtattgctg accatactac aggaaatgaa acaggaagaa tgatggtggt
     7501 aaatggtttt aatcctggag ctgtattttt taaatcaaca gtacaagtta cacctaacac
     7561 taattattta tttagcactt ggattttaaa cctttttaag gtaacaggat atccaccacc
     7621 acagctaggt gtaagaatat ttgaccaaaa taataatata ctatatcaag caacattagg
     7681 agctcaaatt ccagttaata caaacgcacc agaatggaaa gaagtaggta ctgtgattaa
     7741 ttctcaaaat aataatcaac ttacagtaga attttttagt gaaggagaaa gcgccattgg
     7801 aaatgattat gctatagatg atgtttcttt aagagaaata gctgttccaa cttttactcc
     7861 aataaaaaca agtaacacag aaactgctaa tgtaggtgat ataatcacat atacagttac
     7921 attagataat acttgtgata gccctttaac taatgtattt tttagggatt taattccaaa
     7981 tggtttagag tttatacctg gaactgtaac ggttgatgga gtatctgtac caggagtaga
     8041 ccctaataca ggtttctcac ttccagatat tggaggagga acaggggttg aagtaacttt
     8101 tgatgttgtg gttgaaagta ttcctaaccc aaatcctact aataatatag ctaatataga
     8161 ttactcctat acacctgtag aaggcggtat accaaatgat ttttcagtag attctaatcc
     8221 agttcctgtt gaagtaatat cagcagatat tgaagttaca aaattatctg aacctactat
     8281 agtgaatcca ggagaggaat taatatacac tataaaagta gtaaataatg gaccattccc
     8341 atctgaaaat gtggttttaa cagatgatgt accagcttct attataaatc cagagtattc
     8401 attagatgga ggagtaactt ttcaaccatg gactggaagt ttaaatatag ggacattaga
     8461 agtaggagag acaagagtta ttataattag gggtattgta aatccatcaa cagtaggaat
     8521 tataacaaat acagctgtag tatcttcaac tacagcagac ccaaacctta ataataatac
     8581 atctacaata gagacagaag tgaatttatt agcagatatt ttagtaatga aaacagcaga
     8641 acctaattca gctgttccag gtactttatt gagatatact attcaagtag aaaacttagg
     8701 accagcaaat gctgaaaatg taatattaaa tgatgatatt ccagcttcta ttataaatcc
     8761 agaatattca ttggatggag gagcaagttt tcaaccatgg aatggaagtc ttaatatagg
     8821 aactttaaac tctggaattt cattaacagt tttaattcaa ggaacagtta gtttaaattc
     8881 ttcagaatat atagtaaata cagcaacagt ttcttcaact acacctgacc cagatttaag
     8941 caacaatata tctaccatta ttacaccagt taacccacaa gcaggtattt ctattataaa
     9001 agtagctgac gaggatgtag ctgttccagg agaagaattt gtatatacaa ttgaaatttt
     9061 taatgaagga ccatctaatg ctacgaatgt agttttaaca gatgatatac cagatgttat
     9121 tttaaatcca gaatattcat tggatggagg agcaactttc caaccatgga atggaagtct
     9181 taatataggt acagttgcac cagggcaatt aataagaatt atcataaagg gattagtaag
     9241 ttcaactgct acaggagata ttactaatac agcagaagta agtgcagatg taccagaacc
     9301 agttacggat tcatcaacag tgacaactcc tatagttcca tcagcagata tagaagtaat
     9361 aaaaactagc aatatggata cagcagttcc agaagaaact ttcagttata ctataacagt
     9421 tacaaattta ggaccatctg ctgcacaaag tatcgttttg acagatgata taccagatgt
     9481 tattttaaat ccagagtatt cattggatgg aggagtaact ttccaaccat ggaatggaaa
     9541 tcttagtata ggcactttgg atgctggaga aataagaagt attataataa gaggaacagt
     9601 gagccagaca gcagtaggta caattataaa tacagcaaca ataagttcac ctacacctga
     9661 cccaaatcca gataataata cttcaacaga tgaaacagat ataagtccat tagcagatat
     9721 ttctgtgata aaaggcaacg aaccagttgc tataccagga ggaagattta tttatggaat
     9781 tgaaattgca aatgcaggac catcttttgc agagaacgta actttaacag ataatatacc
     9841 agcttctatt ttaaatccag agtactcaat agataatgga gtgactttcc aaccatggaa
     9901 tggaagcctt aatataggta ctttagatgc tggagagata agaaatatca taataagggg
     9961 aacagtaagt caaacagcaa taggtacaat tataaataca gcaacagtaa gttcaactac
    10021 acctgaccca aatctaaata acaatacttc aacatcagaa gcagaggtaa gttcatcagc
    10081 agatatttct gtagtaaaac gaagcaatca gactgttgtt gtaccaggag atgtactaga
    10141 ctatacaatt gaggtgagaa atgcaggacc gtctactgct cagaacgtaa ctttaacaga
    10201 taatatacca gcttctattt taagtccaga atattcaata gataatggag tgacttttca
    10261 accatggaat ggaagtctta gtataggcac tttagatgct ggagagataa gaaatatcat
    10321 aataagggga atagtaagtc aaacagcaat aggtacaatt ataaatacag caacagtaag
    10381 ttcaactaca cctgacccaa atccagataa taatacttca acatcagaaa tagatataag
    10441 ttcatcagca gatatttctg taataaaatt agcaaataaa acagaagcat gtgtaggaga
    10501 gcaaatagat tttgtaatag tagtatcaaa tgcaggacca gaaaatgctc aaaatgttac
    10561 tttaatagac aatgtttcag ataagcttaa gaaagcagta ttttcacttg atagaggtgt
    10621 aagtttccaa ccatggactg gaagtcttaa tataggtaca ctaccagctg gaactttaag
    10681 agtgattata ttaagaggaa ttataaaatc aacatgtctt gatagattaa caaatattgc
    10741 agaagttact tcaacaacac cagattcaaa ccttaacaat aatatatcaa gagtgcaagt
    10801 agaaataaaa caattttgtt gtaatgattg tggttgtaaa gattgttgct gtaattgttg
    10861 ttgcaatgat tgtggttgta aaaattgttg ctgtaattgt tgtaaaaatg acttttggtg
    10921 ttgattttat aaaataattt tgtatacctt aaattaaact gaaataaaaa ataaattcac
    10981 atatgagtaa aatattttat aaatattaat aaattttcta tctttgatat ggaccacatt
    11041 cgtaatacta gttattaaat gaagtggtcc tttttaattc attaaaatta tttattctaa
    11101 ttaaatttt
//
LOCUS       DAMJWS010000110        10852 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMJWS010000110 DAMJWS010000000
VERSION     DAMJWS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.115"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(67..1056)
                     /locus_tag="PC399_003310"
     CDS             complement(67..1056)
                     /locus_tag="PC399_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF2594138.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            1496..2251
                     /locus_tag="PC399_003311"
     CDS             1496..2251
                     /locus_tag="PC399_003311"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2594139.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHDTLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            complement(2605..4281)
                     /gene="bclA2"
                     /locus_tag="PC399_003312"
     CDS             complement(2605..4281)
                     /gene="bclA2"
                     /locus_tag="PC399_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF2594140.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGVTGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGENGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVTQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(4659..5198)
                     /gene="hpt"
                     /locus_tag="PC399_003313"
     CDS             complement(4659..5198)
                     /gene="hpt"
                     /locus_tag="PC399_003313"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2594141.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(5526..6833)
                     /locus_tag="PC399_003314"
     CDS             complement(5526..6833)
                     /locus_tag="PC399_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF2594142.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(7258..9033)
                     /locus_tag="PC399_003315"
     CDS             complement(7258..9033)
                     /locus_tag="PC399_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2594143.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(9030..9167)
                     /locus_tag="PC399_003316"
     CDS             complement(9030..9167)
                     /locus_tag="PC399_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594144.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(9245..9814)
                     /locus_tag="PC399_003317"
     CDS             complement(9245..9814)
                     /locus_tag="PC399_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2594145.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(9998..10633)
                     /locus_tag="PC399_003318"
     CDS             complement(9998..10633)
                     /locus_tag="PC399_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2594146.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         3281 a         2038 c         1260 g         4273 t
ORIGIN      
        1 atttaataaa atgaactact aaattttcta tatatcaata aaataaacta ttaaactttt
       61 tataatttaa taaatagaac tgctgtttac cactggttgt gcatctttat tgccacaaag
      121 cacaactaac agattactta ccatagctgc tttacgttct tcgtccaaag tcacaacatt
      181 attttcactt aaattagtca atgccatctc taccatactt acagccccct caacaatctt
      241 tttacgtgct gctattatag cctctgcttg ttgacgttgt aacattgctg ctgcaatctc
      301 tggtgcataa gaaagatgag taattctcac ttcacacact tcaattcctg caatgtcaac
      361 ccttgattgt aattcatctt ttaatctatc tgctatttcc tgactactac cacgaagaga
      421 tttttcatct ccatcttcag atacatcata tggataaagt cttgaaacat tgcgaatagt
      481 agaatcacac tgaatagata agaatgtatt ataattatcc acattaaata ctgcctttgt
      541 tgcatctata accttccaaa ttacaaccac tcctattata attggatttc ccaataaatc
      601 attgactttc tgcttttcat tattaagagt cattgttttt aaagatactt ttttgcccct
      661 tgaattagtt gatatatcta ccttttcatc agatgaagat ttcgatacat taattcttga
      721 tactgctggg tttattgctg aacaaaatgg attcacccaa tagtaccctt ctttttttat
      781 agttccataa tagttaccaa acaaaactag aactatagct tctttaggat taatcatttt
      841 tagcccaaaa aataggataa aatctactac tataaataca agtcccaaaa ttaacataag
      901 tgaacctatt ccagtaaatc cactatcaag ttttactatc ccaaaaacca tgatacctat
      961 tgaaactact aaaaataata aaatcattat caaagctgcc actccattga atacttttgc
     1021 gtctttctct tgataagttt tttcttcttt taacatatct atcactccca tctcagtata
     1081 ttaagttaag atatttctaa tttaataata acacttttaa caattaaatt gttgaattta
     1141 aaattaatac tatatgttta tatatttttt atacatcttt atgcatacat gtatttagtt
     1201 attttaatgt attaatatta atttccaaat aaatcatatg atatatgaat atggtatttc
     1261 aaaattaact ttttagttca tggggcactc tttttggtat gaagtcaaat atatttttat
     1321 catttaaaca atgaaaaaga aatttatcta tattttgaaa aatccactta aattttttaa
     1381 tgtaagataa attgtaagct tattatcact aatattattt tatataaatt atattagtga
     1441 taaaataaac ttatagaaat ataacaaatt tttattaatt tcataggagg aatttatgtt
     1501 gaaagttata attagtggct ataatggaac aatgggaaga gttttagcag aatgtgtaaa
     1561 aaaagataat gaattagagt tggtttgtgg tgtagctagg tctgctgatg aaaaaggtca
     1621 tcatagtgat acaaagcttt acaaaacaat gactgatgta actgaaaaaa ctgatgtaat
     1681 tatagacttt tcacatcatg atactttaga gccaatattg tcatatgctc taaaaaataa
     1741 tacacctgta gtattagcta ctactggata tgacgaaaat gacttaaata aaatagaaga
     1801 agcatcaaaa aacattccta tattattatc atcaaataca tctcttggag taaatatatt
     1861 aataaaatta gttaaggatg cttcaaagat gttagaaggt tttgatattg aaatcataga
     1921 aaaacatcac aatagaaaag aagacgcccc tagtggaact gctactatga tagctaatgc
     1981 aattagagaa gtcttgcctg aaagtgaaaa tacacatggt agatatggta gagattgtaa
     2041 aagaaaacca aatgaaattg gaatacactc tataagaggt ggcaatataa tcagcgacca
     2101 tgatgttgta tttgcaggtg ataatgaagt tcttacatta agccatcatt ctcaatctga
     2161 tgaagtattt gcaaacggct ctatagttgc agctaaattt ttaattggta agcaatctgg
     2221 gctttataaa atgtcagata tattaggata agttttgtac ataataaatc tcaatctatt
     2281 taggtattca tattcatcaa tttgaatatc taattgattg agatttctat tctaaaagtt
     2341 tataaatttt atgcttacct tttaaatata atcatttttc ttaaatatta tcttatttat
     2401 tggtatatga aatcatattg tttattattt tatagtcttt tcatcatata aacctattaa
     2461 atattatcct atttattggt atatgaaatc atattgttta ttattttata gtcttttcat
     2521 catataaacc tattaaatat tattctattt attggtatat gaaatcatat tgtttattat
     2581 tttataatct tttcatcata taaactattg tattctataa actgatacat atccagctgc
     2641 agaagtacct gtcgtgcctg aaccaaatgt aaaacttaaa gtaacagctg ctgctctagc
     2701 atcaactaaa aagcttaaac ttacacttac atctgatgaa gtaagtgtat ttactgcttt
     2761 tgcaataaag ttattttgag ttactccatt aaagttagta gtaattgaca taaatcctgc
     2821 aagtgaaagt gtggcacgta taccgaaaga tacattgtat atccctccaa ctgcaagatt
     2881 tatagtacta cttgttggaa aagttatatt agttccattt ataaatgatg aattaaatgt
     2941 cactaatgta ttattcacta aacttgaaga ggacactgta aattgtgcat tgtttgctgc
     3001 taacactcct gttgctcctg tcgctccggt tggacctgtt gctccatttg ctccggttgc
     3061 tcctgtcgcc cctgttgctc ctgtcgctcc atttgctccg gttgggcctg ttggtcctat
     3121 cgctcctgct actccatttg ctcccgttgc tcctactgct cctgttgggc ctgttgctcc
     3181 atttgcccct gttgctcctg tcgctcctgt tgctcctgct actccatttt ctcccgttgg
     3241 acctgttgga cctattggac ctgctactcc attcgctccc gttgctccta ctgctcccgt
     3301 tgggcctgtt gctccatttg cccctgttgc tcctgtcgct cctgttggac ctactgctcc
     3361 atctgctccc gttgggcctg ttgctcctac tgctcccgtt ggacctattg ccccagctac
     3421 tcctgttgct cctgtcgctc ctgttggacc taccaaacca tttgctccgg ttgctccggt
     3481 tgggcctgtt gggcctacca aaccatttgc tccggttgct ccggttgggc ctgttgggcc
     3541 tgttggacct accaaaccat ctggacctgt tgctcctact gctcctgttg gacctacctc
     3601 tccatctgct cctgttgctc ctgttgctcc tattgggcct gttgccccag ttgttcctgt
     3661 tgctccagtc gctcctatac ttcctgttgg acctgttgct cctgtatttc ctgttgggcc
     3721 tgtagcacct gttactccat ttgctccggt tgctcctgta tttcctgttg gtcctgttat
     3781 tccatttgct ccggttgctc ctgttgctcc agttagtccg gttggtcctg ttgctcctgt
     3841 tgctcctgta ttccctgttg gacctgttat tccatttgct ccagtcgctc ctgtatttcc
     3901 tgttggtcct gtagaacctg ttattccatt tgctccggtt gctcctttgt ttcctgttgg
     3961 tcctgttatt ccatttgctc cggttgctcc tgtattccct gtcggacctg ttattccatt
     4021 cgctccagtc gctcctgtat ttcctgttgg tcctgtagaa cctgttgttc catttggtcc
     4081 agtcgctccc atatttcctg ttggacccgt tattccattc gctccagttg ctcctgtatt
     4141 tcctgttggt cctgttattc catttgctcc ggtcgctcca gttacacctc tgggccccgt
     4201 cggtcctgtt ggaccagtag cacctgttgg ccctgttgga cctacatctt gatataaact
     4261 tggacctgaa atatcactca tataattaat cctccttttt taaagttaga gtaatactct
     4321 ataaatattg agtattactc tatataatta tatgaatcga caatttctta tgtgataatg
     4381 tatctatttg cttatatcaa catatatgct ttgattctta aatttcatta cttttttgta
     4441 aaatctttta caattattag tactctttta caattattaa tacaattaaa aaactcattt
     4501 cccatcaatt atttaaaatt tttttattat gctataaaaa atagataatt aaattgcata
     4561 aaattaattt aatactactt taattacttt ttaaataaaa aaacctcaac acgattaata
     4621 aaaatcatat taaggcatcc acaaaattat ataattgctt atctatcttc atcagtaaca
     4681 ttgaatatat aaggaacatt tctataataa tcaccaaaat caaacccata tccaaccaca
     4741 aacttatctt ctatttcaaa tccacaataa tctggcacta aatcgacttt tcttctgctt
     4801 ggtttatcca aaagaacaca gcattttaaa ctagcaggat ttttagcttt tagatgtttt
     4861 aacacaaaat ccatagtaag tgcagaatca gtaatatcgt ctacaaccaa aacatcatat
     4921 cctgctatat cagtagtcac atctgaaact actttaacct ttccagtact ttcttcattg
     4981 tttccataac tagatgtagt cataaaatta actctaagtg gtaagtttat atttctaact
     5041 aagtctgcac aaaatataaa gctacctttt aatagagaaa ctaccataag atttttatct
     5101 ttataatctt tttctataat tgctcctaat tcccttaatt tatctttaat tttttcttct
     5161 gaatacaata cttcccattt ttttgtttct atatccataa aaagacctcc aattgtaata
     5221 atccttctta attttataag agattgctca ctttttcaat actcttattc aatattagtc
     5281 aatattattg agtttttatg ttcattcaac aattattaac aatgtatctt atattttaag
     5341 tagttcatat atccacacta aattacatac tatatatttt agacaaaaaa agaaaaaact
     5401 tgcattaaca agttttttct attatacata aatttataca taatcatatt ttattagagg
     5461 gtactaatta aatttattta tgctattttt gttatacctc ataatacaaa acatattata
     5521 aagtactagt caactttatt catatcattc attacattta agattatctc atctgttata
     5581 gacatacctt tgtcacttac tctacctaag ttttgtatac tttgctcaac acttgaacca
     5641 actataccat ttagtggtgg tacaaaacaa tcttgcttag ctattatagc actttgtata
     5701 gctgcagatg ctgcagaaga taattttaat gcacaaccag ctttcgcacc gtcacatatc
     5761 attccactta agtcagctat catattttct atagctcctt ctatttgctt ttcagtacca
     5821 ttcataagcc aagagatacc agcagttgct ccagtagaag cagcaacacc acatccacat
     5881 acagcagata atcttcctgt atagttctta atataaccag ttactagatg agatatagct
     5941 aaagcttttg ctagtctttc atcagattgt gggaattttt tattataagc aacgattgga
     6001 agaattgctg ttaatccatg atttccactt ccatttgaac tcataacagg catttttacc
     6061 cctgccattc ttgcatctga agctccagca gttaacatca ttgcataatt tataacatca
     6121 ttacctaaaa gaccttcttc tatagacttt ttaattccat atcctacacc aataccagtt
     6181 ttttgtttta atccatattc agccatctct tcattcattt ttactccatc tagtaaaaat
     6241 tctatatctt taaaatcaat tccttctaca ttttttatta attcttgtat agttactgta
     6301 tccattaaac tttcagcttt ttctgccgca tcatttgaag ctgatacttt tggttcattg
     6361 tctaataata cttctccatc tttttctaag aacgtaaaat tatcatgttt agttcttatt
     6421 ctcactttag caatatgatt ttttcctttt aacacaactt ctataaatac tttctctctt
     6481 gtatcagcag gtgttattga tagtggagta ttatccatat aatcctcagc tattttaact
     6541 tcctcttttg ttagagtttc taacacactt aatccatttt cagaatgtcc acctactata
     6601 cctagagctg ctgcaatttt taatcctaat ctttcagtac ctggtatacc tacgcacata
     6661 ccatttttat aaatactagg actaactaac atacgatttt caactatttc ttctcctaat
     6721 aattcctttg cctttgcaca tgctagagct aaggctactg gttcagtaca ccctactgct
     6781 ggtttaactt ctgcctttaa catttccact aattcttttc ttatatctct cattatatat
     6841 aaacctccca attaagttta gatttagtac tcaaataatt aattaaataa tttcttatct
     6901 atatttatag caaaatacat gccatatttt ttatatcttt ttatattttt agtcttatat
     6961 tctataacca ttggtatgac tgcctctatt tatgtatttt ttttatttgt atagagtatt
     7021 tatattcttt aaaatggaga aacatatgac aatttcgcaa aaagacaaaa aaaagtctca
     7081 aaatgagatt ctatatcatt ttgagacggc tcaaatatat tattttttaa cttataaaaa
     7141 ccatttttta attcataaaa aaagattctg caaaacactt atagtataag tttaaagctt
     7201 tctttaaagt tatgaattta gaaaaattta aattttatat agctttttat tatattacta
     7261 tgaaattata ttgtattttt caatttttct atataatgta gctcttgata ttccaagtgc
     7321 tttagctgtt aattctttat cttttttata atctttgtac ttatttaaag cttttattat
     7381 ctcaatcttc tctagttcat caagtggaat tatcctatcg cttttaactt ctaactcttc
     7441 tacagaggta ttcttaattt tttgaggaat tacattcatt gtaattattg aagaagaaga
     7501 catattcaca ctatattcaa taatattctg caactctctt atattccctg gccacttata
     7561 ttccatcata gcatcaacaa ccctgctatc tatgtcaata acatttttat ctaatttttt
     7621 tgaatactct ttaaccatat gcttgattat aagtgggata tcttcttttc tctctcttaa
     7681 acttggtaat tttataggta taacatttaa tctatagtat aaatcttctc taaaagtgcc
     7741 attttgaacc attttttcta aatctttatt tgtagcagca ataattctta catctattga
     7801 aacatttgat ttacttccaa ttttattaag ttccttttct tgtaatactc tcaataactt
     7861 tgactgtaaa tgcaaactca tatctcctat ttcatctaaa aatatacttc ccttatgagc
     7921 tacttcaaac attccaagct ttccaccttt tttagctcca gtaaaagctc cctcttcata
     7981 tccaaataat tcactttcta ataaattgtc tggtatagca gcacagttta cagctataaa
     8041 aggattatct actctattgc tatggttgtg tatggctctt gcaaatagct ccttacccgt
     8101 tccactttcc ccagtaataa gtactgttga tgttgattta gaagctatta gggcttcctt
     8161 tttagaattt cttatagcta cactttctcc aattatgttt tccaatttta ttctataatc
     8221 tttattcatc ttattgtagt tttttatagc atctctttta tctataaaat caataacata
     8281 tccctttaac tcatttttta atactatttt attgatgttg tatattccct tcgaatcata
     8341 tccgtatctt ttataaaaaa aactacagga tttgtctttc ttaaagttat ttatagaagg
     8401 tttctttata aaatctaatt cgacaaatat gtttttgcca gttatactat cctttatatt
     8461 gaaaagctct ttaaattttg aattatattt atcaattttt ccatccacat caatagacac
     8521 aacagctttg tccatattgt ttaacagtgt ctctaatttt ttcttttcta attcaagctc
     8581 ataagtttta atttgagctt ttaatttatt agatattaag tctgccattt tgcctaaaaa
     8641 gtttataaga ccacttttat tattttctat aatctgtgct tgttcctttg taaaggctat
     8701 aaggcctata acaccatgag agactccatc acatataatg ggacaacaca cctctgcaaa
     8761 ctctacacaa gccccacttt tatagcattc cctacatata tcactactct ttgggtcatc
     8821 aattagtatt gtaatctgct ctgtaagtga ttttttaaaa gccgtatatt tatctacttt
     8881 ctcacctatt ttgtctatat atactcctgt acctgcaatc ctaaataact ccttatttac
     8941 tatagttaca tctatattga gtactgatac tatagcttca gctatatttt gaatatcaga
     9001 ttttatatat tctaaaccta tcataatcat cattccttat ttttctttaa cataagtatt
     9061 tgtcttattt ctttaagaat caagtatatc ttatttaaag taaattggat tgacatcagg
     9121 attacaaaga gcataacaat tattattaac tcataattta tttccatcta aacatctcct
     9181 tataacaaaa gactaggctt aacctagtct tttatattat atccatattt atattttaac
     9241 tcatttatct tctttttccc acataaatat attttaattt atttgactct ttaatcatac
     9301 catcaaagtt taatcttgaa aatatattaa atacttcatg tggatagtga atctttgcaa
     9361 cagaaccaca aaatttatgt ttaagatagt ttaacttaac ttttaaattc tttaaattta
     9421 aatcctctaa tataacctct ccattgccct ttaacactct ttgtatttca gatgaagctt
     9481 tttcacaatc tgtaataaca ttaaattggt caactattat tatcttgttg aactgattat
     9541 catcaaatgg tagaaactct aacttacctt taactacatt gcaattttca ttatacacta
     9601 gagaataaca gacatcttca taattactat tctccagtat agttacagaa gttccaagac
     9661 ctctcaatct cttgcctagg tttcctatat gaccaacaag caatatttta tcacctacat
     9721 tatactcaat tgtatccaaa aaacattcta attcatcaga tgtatattta gctttaaccg
     9781 gctgtttatt tatctgacat aatatgcttt tcacaatgct tcacctcttc atttcctcaa
     9841 ttataaatta tgtacagatt acattataat aaatggcgag caactattca agagatattt
     9901 cattagtatt tttttaaaca atctttatac attttaatat gatattatac caaatttatt
     9961 aggattttat tcataaaaat cagtctattt tttatgttta ttcattgtta ttttcatcca
    10021 atttttttat tttcgatatg gatacttcat atgccatttt tttgaccaca ttattttcat
    10081 ctattttttt ctcatattct ctactttgaa ccctacccca aagctgtatt ctatcaccaa
    10141 cctttagatt ctttgcaaat ttagcatttc taccccaaac tattgatggt atataatcag
    10201 atttattata aggtctgttt atagcaacta ataaatcggt tatttccctt cctaaaggtg
    10261 tcttcctata cacaggttcc ttacatacat atccatctaa aaatatacta ttagggtctt
    10321 tattttcctc gtctatgctt tttatttctc tgacaaacac tgtaagcact aatctatttt
    10381 tattatttaa tgttttgtta tatgatctta attggcctac gacattgact aaattttgtt
    10441 tatcaaggtc aatatcttgg agaagtcttt ctgatacagt cattggaagt atatcaaaag
    10501 aatcgcttag tctgtttatt tttattttca tattataaaa tttttctcca aatatctcat
    10561 ggctaaattc tagcttattg tctaactccc ctcttaagtt tactacgtta ttctcatctt
    10621 taacagctat catattttat ccctccacaa ttgttaattc ttatatttct ggtatcagct
    10681 tatataataa atatatttat aagggatgta ttttattaca aatctttgat tgctgtttaa
    10741 aagtttttat ttagttataa atttcattta atatttcact tttataatta taaaattatt
    10801 atctttttgt accacatata ctaattaaac tacctatttc agttttgtat ta
//
LOCUS       DAMJWS010000111        10818 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMJWS010000111 DAMJWS010000000
VERSION     DAMJWS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.116"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            84..851
                     /locus_tag="PC399_003319"
     CDS             84..851
                     /locus_tag="PC399_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF2594147.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1069..1282
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1536..2570
                     /locus_tag="PC399_003320"
     CDS             1536..2570
                     /locus_tag="PC399_003320"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF2594148.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2611..3828
                     /gene="argJ"
                     /locus_tag="PC399_003321"
     CDS             2611..3828
                     /gene="argJ"
                     /locus_tag="PC399_003321"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF2594149.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEAHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3843..4703
                     /gene="argB"
                     /locus_tag="PC399_003322"
     CDS             3843..4703
                     /gene="argB"
                     /locus_tag="PC399_003322"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF2594150.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4733..5920
                     /locus_tag="PC399_003323"
     CDS             4733..5920
                     /locus_tag="PC399_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF2594151.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYVERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6029..7051
                     /gene="argF"
                     /locus_tag="PC399_003324"
     CDS             6029..7051
                     /gene="argF"
                     /locus_tag="PC399_003324"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF2594152.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7178..7795
                     /locus_tag="PC399_003325"
     CDS             7178..7795
                     /locus_tag="PC399_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2594153.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8015..8713)
                     /locus_tag="PC399_003326"
     CDS             complement(8015..8713)
                     /locus_tag="PC399_003326"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF2594154.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8875..10092)
                     /locus_tag="PC399_003327"
     CDS             complement(8875..10092)
                     /locus_tag="PC399_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF2594155.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10283..10696)
                     /locus_tag="PC399_003328"
     CDS             complement(10283..10696)
                     /locus_tag="PC399_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2594156.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
BASE COUNT         4233 a         1183 c         1809 g         3593 t
ORIGIN      
        1 gatgttgttg tattcaaaat attaagataa aaatatatag aatatattta aaaattatta
       61 tgcaattaga aaaggagata agtatgcaga ttattgatat aaatataaat aatattgaca
      121 atgaacatat atgttgttca atttctgaaa aaaaaggaga aaattgtgtt ttttctaaaa
      181 aagcttggat gaaaaataat tttgataaag gtctagtctt taaacgattt gatgtaagag
      241 gtaaagcttt tatagaatat attccagcag aaaatgcatg gtgtcctatt actgcggaca
      301 attatatatt tattaactgt ttttggatat ctggaaaatt taagggtcaa ggatattcta
      361 atctattgct acaagagtgt gttgatgatg caaaatcaaa aggtaaatat ggattagttg
      421 tgctttcgtc tagtaaaaaa atgccttttt tatctgatgc taaatatcta aaatataagg
      481 acttttgttt agcagataca tcagaaccaa attatgagct attatatttt ccctttactg
      541 ataatgctcc tattccatgt tttaagaatt gtgtaaaaca aagaaaatta aatgagaatg
      601 gtatagtttt atattacagt aatcagtgtc cacatacgga aaaatatgct cctattttga
      661 aaaaaattgc aaattctaga ggtattgatg taaatcttat aaaattcgaa acagcaaaag
      721 aagcacaaaa tgctcctgca ccatttacta catacagtct tttttttaat ggtgaatttg
      781 taaccaacga gatactttct gagaaaaaga ttgagaaatt tttagatacc aacattaaaa
      841 aatttaatta aaaagagatg taaatattgt attttgaaaa tatattattt tataatttaa
      901 tatattttgg atgtttttta aattgacgaa tgaaatataa aaattatatc gcaatttaga
      961 tacaaaatgc tggaaattaa ccatatagaa aatatttcca aaaatttaaa aattatattg
     1021 acaaagttaa aaaaataaac tatcatataa aacaataaga aaataaattg aaatcctgtg
     1081 aaaaagagag tagcataaag ctagttgata gagagctggg aatggtggaa gcctagtaac
     1141 gaattttatt gtgaatagag ctttggagga gattaccaga agtaatagta tggtaattgg
     1201 tgaatctcgc cttaagagat tagagtgggt aagattatct tatcaattag agtggtaacg
     1261 cgggtgtata ttcgtctctt ggtctttgac caagaggctt ttttaatttt aaatgtacat
     1321 tttgtagata ctttttaatt tgagttttaa tgattattgt aattttacca ataagagtgg
     1381 taccgcggaa aatcgcctct tagtatttat aatgctaagg ggttttttgt tttaattaga
     1441 gtattttaat ctacgtatta tcaaatgatt atcatatagt aaagtggaaa gataggattt
     1501 taagactatt tgaatataaa aatggaggga acaaaatgaa taaaataaaa gttggtatta
     1561 ttggtgcaac tggatatgtc ggggcagagt tgattagatt attaatgaat catgataaag
     1621 tcgaagtgac agcgataggt tcaaattcgt atgttggaaa agatatagtt gatatatatc
     1681 caagtatagg atataaaaat aacatgatat gtatagaaaa tgaaaaagtt atagatatgt
     1741 gtgatgttgt gtttacagcc cttccacatg gagttagtga aaagtttgta ataaaggcaa
     1801 ttaaatcgaa aaagaaagtt atagatttag gtgctgactt tagaataaaa gatgaagaag
     1861 tatatagtaa atggtatgga gtatctttta tagataaaat cttacataaa aaagctgtat
     1921 atggattaag tgaaatatac aaagaagata taaaagatgc agatataatt gcaaatcctg
     1981 gatgttatcc aactagtatt tctttaccat tgatgccatt actaagttct aaattgataa
     2041 aaaataataa tataataatt gattctaaat caggtcttac aggtgctggt agagaattat
     2101 cgattagtag tcactttacg gaggttaatg aaaatatcac tgcatataaa atagggaaac
     2161 acagacatac tccagaaata gaacaaaatt taagtgaatc ctgcaaagaa aaagtaagtg
     2221 tagtttttac accaaactta atcccagtaa ataggggaat cttatcaact atatattgta
     2281 caaaagaaga taatatttct ataaatgata tacataggaa attaactgat tattatgaat
     2341 ttaaagaatt tatagaaatt cttccattag ataagatagc ttctttaaag aatgttagat
     2401 tctcaaataa atgtgtaata tcattacatg aaaatggaga tacattgata atctgttcag
     2461 ctatagataa tatgattaag ggagcagcag gtcaagctat acaaaacatg aatataatat
     2521 ttggtattga agaaaataca ggtttaaaaa atattgcacc atcattttag aaataaaaaa
     2581 tactaaactc atataattag gaggataaat atggagatta taaagggtgg agttacagtt
     2641 tcagaaggat tttttgcttc agggattcac tgtggcttga gaaaaaataa agaaaaaaga
     2701 gatttagctt tggtttattc agatgtttta tgtgatgcag cagcagtagt aactcaaaat
     2761 aaagtaaagg gcaatcctgt ttatgttaca caggaacaca ttaaaaatgg aaaagcacaa
     2821 gctatcatag taaatagtgc taatgctaat acctttaatg gaaaagaagg acttataaat
     2881 gcttataaaa tggctaaatt caccagtgat aaattaaata ttaaagaaag tgatgttttg
     2941 gttgcatcta caggtgttat aggaaagcca ttaaatatag atttaataga agaaaatatg
     3001 gatgaattag tgaataatct aagtaaacaa ggtcatattg gagcaagaga agctattatg
     3061 acaactgata ttataaaaaa agaaatagca gtagcaataa tgtatggaga taaaaaaata
     3121 actattggag gtatggcaaa aggttctggt atgatacatc ctaatatggc aacaacgtta
     3181 ggatttataa ctacagatgc aaatattgat ggagttttgc taaaagaagc tttaaaaatt
     3241 gctgttgaca aaagttttaa tagagttagt gtagatggag atacaagtac taatgatatg
     3301 gtaatgattc tagcaaatgg aaaagcaaaa aatgatagaa taaacaaaaa agatgaacat
     3361 tatcaagtat ttttgagtgc acttacatat gtctgtatag aacttgctaa acttgttgca
     3421 aaagatggag aaggatgtac aaaactaatt gaatgctata taaatggagc attaagtgaa
     3481 gcacatgcag taaaattagc aaaaacagtt atatcttctt cattagtaaa gacagctgta
     3541 tttggagctg acgcaaattg gggtagaata ctgtgtgctt taggttatgc aggtgaagaa
     3601 atagatatgg aaaaagttga tattattttt gagagtatga aaggatatat tgaagtatgt
     3661 aaaaatggaa atggattaga ttttaatgag gaaaaagcaa aaaaaatcct tgaagatgac
     3721 gaaataagta tattagtaga tttaaatatg ggcaatgcca gaggaaatgc atggggatgt
     3781 gatttaagct atgattatgt aagaataaat ggaagttata gaaattagga ggatatactt
     3841 taatgattaa tatagaaaaa gcaaatacac ttatagaagc tttaccatat atagaaaaac
     3901 atcagggaaa gactattgta gttaagtatg gtggaagtgc aatgaaaaaa gatggtctaa
     3961 aagaatctgt tatggaagac cttgtgttaa tgagttatgt tggaatcaat atagttttag
     4021 tacatggtgg aggggcagaa atcaataaga tgctagcaaa agtggatata gaaagtaaat
     4081 ttgtaaatgg tcttagatat acagatgaag agaccatgga aattgtaaaa atggtacttg
     4141 ctggaaaggt taataaagac ctagtaaata aaatacatac aaaaggtgga aaagcagtag
     4201 ggctttgtgg aatagataat aatatgattt tatgtgaccc atataaaaat tatgaacttg
     4261 gatttgtagg agaaatcaaa aaagtaaatg ttgagcttat agaatcttgt ctaaagtcag
     4321 gttatatttc agtaattgca actataggtg ttggagatga tggagagact tataatataa
     4381 atggtgacac agcagcttct gctatagcta aagagttaaa tgcagataaa ttgatacttc
     4441 ttacagatgt ccctggtttg ttaagagagc cagatgaaga aaaatcatta ataacagaag
     4501 ttatattaga ggatgttgat aaattatttg aagaaggtat aattactgga ggtatgatac
     4561 ctaaaataga gggatgcgta gatgccttaa ataacggagt aaatagggta catattttgg
     4621 atggaagagt tccacattct ataataacag agctatttac tgatagtgga ataggtacat
     4681 taataagaaa agaaaatgaa taaaattata agtttaagag gggagaataa gaatgaataa
     4741 cgacaataca atttcaaaat ggaatgaata ttttatagat acatataatc aacccaattt
     4801 tgtcatagat tatggtgaag gaagttgttt ttttgatact aatggcaata aatatataga
     4861 ttttacaagt ggatatggag tatcttctct tgggtatagt aatagtaatt taaagaatgc
     4921 tctaaaagag caagtagata aattattaca tacttcaaat ttatatttta atgagccagt
     4981 gttgtattca ggagagaaaa taattaattc aagtggaatg gctaaagtat atttttgtaa
     5041 ttctggaaca gaagcaaatg aaacagcatt caaaatagca agaaagtata gtagtgacaa
     5101 atatggtaat ggtagaggaa ctatcataag tttaaaagac tcttttcatg gtagaacaat
     5161 gatgtcttta atggcaactg gtatggacaa ataccataag tatttttatc ctcttcccga
     5221 aggttttaaa tatgtagaaa gaaataatat tgaagattta aaaaataatc ttgattccac
     5281 agtttgtgca ataattcttg aagcgattca aggcgaaggt ggagttaatg ttcttgaaaa
     5341 agattatgtt ttggaaatag taaaaatatg tcaagaaaag gatattgtag tgatttttga
     5401 tgaagtccaa tgcggtatag gaagaactgg taaacttttt ggctatgaat attttgatgt
     5461 aaaaccagat atagttaccg tagcaaaagg gttgggagca ggaataccag taggaggagt
     5521 attagtaaat aaaaaacttt caaaagtact aggaaaagga gaccaaggta caacttttgg
     5581 aggtaattta ttagctatgg tggcagcttc agttgtatta gatgagatat ctaaagatgg
     5641 tttttataat gaagtattag aaaaaggaaa ttatataaga aaatctattg aaagttttaa
     5701 taacaaagtt gtattaaaga caaaaggtat tggtctaatg ataggaattg aaactaatat
     5761 tgaatctagt attatagaag agaaagctag aaaaaaagga ttgcttattt tgactgctgg
     5821 aaaaaatgta ttgagatttt taccaccatt aacaatcagt tataaggaaa tagatgaggc
     5881 tcttgaaatc ctaaaagata tattactaga aattaactaa atattcaaga aatagttgat
     5941 taaagataaa aattagaagt aaataaaaaa gtaaagtgct aacaagttta attgaattta
     6001 aaaagtttat aagaaggggc gaattaatat gaataatata agtttaaaag gtaagagttt
     6061 tttgacatta aaggatttta caaaagaaga gattagatat ttattaactt tatcaagtta
     6121 tttaaaaact aagaaaaaag ttggtataaa aggcgatttg ctacaaggaa aaaatatagc
     6181 tttattattt gagaaaacat caacaagaac tagatgttca tttgaagtag cagctcatga
     6241 tgaaggagca catgtaactt atctaggtcc aaatgattgt catatgggta aaaaagagtc
     6301 tgtatcagat acagcaaaag ttctaggaag attctatgat ggaatagaat ttagaggctt
     6361 taaacaagaa acagttgaaa gtctagcaaa atattctggt gtgcctgttt ggaatggact
     6421 tacagatgaa tatcatccaa cacaaatact ggcagatttt ttaacagtaa ttgaaaatgt
     6481 agataaagat ttgaataaag ttaaatttgt atatgttggt gatgctagaa ataatatggg
     6541 aaactcactg atgataggat gtgctaaaat gggaatacat ttcgtagcac tagcacctaa
     6601 ggaactttgg ccaaatgaag aattggttaa ggaaatgaag gaaatagcca agaaatccaa
     6661 aggagatata atgttgactg aaaatattga tgatgttaaa ggtgcagatg ttatatatac
     6721 agatgtatgg gtttctatgg gagaagagga gcaatatgaa gcaagaataa atcaacttaa
     6781 agattatcaa gtaaatatgg atatgataaa gaaaactgaa aatgaaaatg ttatattttt
     6841 acactgtctt ccagcatttc atgatttaga aactaaggtt gctcaggaga tttatgaaaa
     6901 gttcggatta tcagaattag aggttacaaa cgaggtattt aattcaaaat attcaaaggt
     6961 atttgaagaa gctgaaaata gaatgcatac aataaaagct gttatggttt ctactatgac
     7021 tgatgtaaat atgaacaata atttaatata gatatgtttt aatatagact ttactttaat
     7081 gaaaatttat aaaatatttt aaatatatta ttcaaaaact ctcattatat gttaaaataa
     7141 cttatgtatc aacaaattaa gagggaggat ttgagatatg aaaaaaatca tattagcttt
     7201 aacaactttt ttattagcaa tatctttaac agcatgttca agtgcaaaac cagaagatac
     7261 aattgatagt ttttttaaca gtgcgaaaaa gtttgacttt gaaggcatga ataaggtaat
     7321 ggaaaataat gacgaaaaat ataaggatat attaaaggaa ttagatacaa aagacccaaa
     7381 tgcccaatat gttttagatt atctaaaaca gaatgcatct aaaatcacat atacaataaa
     7441 agacagcgag gttaaaggtg ataaggctac aatcaaagta gaatgtaaat ttatagattc
     7501 aacaccatta cttcaagaaa tagtagctga ggcatttaca aagatgattg gtatgagttt
     7561 ttcaggacaa gacttaactg atgagaaaac aactgaaatg ttagtatcaa ttatgaaaga
     7621 aaaacaaaaa agcgttaaag agacttatgt tacaaaaaat gtagagtttg aatgttctaa
     7681 aaaggataat aaatggataa taagttcagt aaatgatgca gtagctgatg tattgttatc
     7741 taatcttgtt acagcaggac aagaattttc taactctatg aatacacttg aataatattg
     7801 tttaggtgta gtaaagtaat aaatatatga ttatttttat aaataaacat ataaatattt
     7861 atgaaggata ccatttttgg tatccttttt attaatataa tgaaatacat ggttaatttc
     7921 tatattgtca agaaactatg atatatgtta taagtctata ctatttaaaa taaacttata
     7981 acatatattt tttataatat agtgcatgaa aaatttattt aactaatttt agatatttat
     8041 tgacaactga ttcagccaag cattttgatg aatcaacaat atttattgaa ggatatgatt
     8101 tttctaaaac tacatttaaa tcagtacaag caattactat attttctata gattcatttt
     8161 taacatcttc gataagttta ttccaaatat caatattgtg tggattttct ttatccattt
     8221 ttatattttg aataagattg tttaatttta tttgccattc atctttaaat ataaattcat
     8281 gtccattatg tattatacca ttctggtaaa ttgtagactc aaaagttgaa ctagtcgaaa
     8341 aaagagttac tttttgtgag atagtaggta attttttaac tgtttcttct acaatattta
     8401 atattggtat attaatagac tcttttaatt ctttaaagta tatatgtgct gaattgcatg
     8461 gcattgcaat aaaatttaca ccagttgatt caagcttttg taatccatca attatagttt
     8521 ctttcataag ttcatgattt attggacggt caatataaaa tggggtaggt aatgaatata
     8581 tcatcatctt tggaaattca tcatcatatt ttgctccata ttgagattga cactcatcaa
     8641 taattaaatc aacaaaaggg gctgttgacc ttggacccat tccagccaaa ataccaatta
     8701 atttttcatt cataaaaatt atctccttta tatataaata tatttatgta taaaataaaa
     8761 atctataaaa gcataaaatt atattttata atagattata ttgtacactt atataatata
     8821 aatataaagt gattttttag attatatatt ttaatatact ttcttatata agaattattt
     8881 taaatcaaga ttatctagta tttttaaaaa tatttttaca gcagtatcca agtcatctac
     8941 agaaacactt tcattaggat tatgacttac gcccccagcg catcttataa ataccatacc
     9001 aatatcagtt atattatcca tttcctgagc atcatggcca gcaccactat aaatataaaa
     9061 aggattaaga tttaaatcta taaaactttt ttctatgatt tttgttattt tattagaaca
     9121 tggtacagga acattttcaa aagcaagttc acttgtataa ttaagttttc tattttcaca
     9181 tatatgtgat atttcattaa atatcttttc tacagaatta tcaagaattt cttgtgaaca
     9241 agagcgtaca tcaagagtaa attcagctct ttcaggtacc acatttgatg aagatggata
     9301 gaaattcatc tttcctacag tagcaaccaa atcagcagtc gttttagcta ctttttcgat
     9361 agctaataca cattcacagg cacaacatcc agcatcaagt ctcatattca ttggaagtgt
     9421 accagcatgt ccagacttac catttactga tatcttaaaa cttttaaatc ctgtaatact
     9481 tgatacaatt ccagcaggaa gagattcatt ttctaaaaca ggaccctgct caatatgaaa
     9541 ttctaaatat gcatctacat cttttctagg atgcaaattt gttaagtttt ttgtattaaa
     9601 tccaaacttt gttacagctt cttcaagtgt aattccattt tcatcaacag atttaagaag
     9661 ttcctcttta aaagtaccag caaaaacttt acttcccatt aaagaagttt caaatctagt
     9721 tccttcctct tcagcgaaag caattagctt aatattgaaa ggataagacc tattattgtg
     9781 ttttgcttct tcaagaccaa ctagaggcac tattatacct aacataccat caaatatacc
     9841 tccacacttt acactatctt gatgagatgc aactactaat gtgggagcat ttggaaagct
     9901 tgatttatat gtacctataa cgtttccaat gtcatcaatt tcaatatcaa gatttaaacc
     9961 ttccatccat gaactaatta aatcaataga ttttttatgt tcttgagtca agataagtct
    10021 tgtaagacca tttgttgttg aacttatttt tcctaatgtt tgtagataat ccatacattt
    10081 ttttcctaac atatttacct cctaaaaata taatttttat ttaagctaag attagctttt
    10141 atacttataa tttttgaact ttcaaataat tttgtatgta tagtaaaata ctattttagt
    10201 attaataatg atataaaaat ataaagtctg tagacgaaga gcagaagttt taagttaaaa
    10261 atataaaata tgatttttat tattatagta tttttctaaa ataatatctc tttccagtta
    10321 gaggataatt ttcaagtgaa aatactttat ggtatccaaa tttctcataa aatttaggtg
    10381 cttgaaaact gaatgtatct aaaaaaacat atttacaacc tcgttcttta gcaatttttt
    10441 cagccttata taaaagttga cttccagtac ctttatatct aagcttctca ctaacccata
    10501 agtattttat agaaagccaa ttaccatgag taacacctat aaaacctgca acaattttac
    10561 ctgctttatc tcgaatatat attcctaaat ccactgggtt tttatcttca agtctagcaa
    10621 gattatattt taataatcct tgaaaaatag tatcttcatc ttgtttttct attttttctg
    10681 taatttctaa ttccatattt gaactttctc cttgtataat cttttgtata ttggtatttt
    10741 ttaataaaat cagtagaaag aatatagttt aacattattc taataggcat ttatgctaat
    10801 gtcaaactaa taatactt
//
LOCUS       DAMJWS010000112        10780 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMJWS010000112 DAMJWS010000000
VERSION     DAMJWS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.117"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(160..492)
                     /locus_tag="PC399_003329"
     CDS             complement(160..492)
                     /locus_tag="PC399_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF2594157.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(736..1377)
                     /locus_tag="PC399_003330"
     CDS             complement(736..1377)
                     /locus_tag="PC399_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF2594158.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFVYSYLMYKISGKNN"
     gene            complement(1358..1651)
                     /locus_tag="PC399_003331"
     CDS             complement(1358..1651)
                     /locus_tag="PC399_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2594159.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2044..2094
                     /locus_tag="PC399_003332"
     CDS             2044..2094
                     /locus_tag="PC399_003332"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594160.1"
                     /translation="MTEYIYYYNNKRIKKN"
     gene            complement(2620..3372)
                     /locus_tag="PC399_003333"
     CDS             complement(2620..3372)
                     /locus_tag="PC399_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2594161.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VAMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3431..5086)
                     /locus_tag="PC399_003334"
     CDS             complement(3431..5086)
                     /locus_tag="PC399_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2594162.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIYIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSVSDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(6358..7437)
                     /locus_tag="PC399_003335"
     CDS             complement(6358..7437)
                     /locus_tag="PC399_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2594163.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(7521..8177)
                     /locus_tag="PC399_003336"
     CDS             complement(7521..8177)
                     /locus_tag="PC399_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2594164.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            8499..8705
                     /locus_tag="PC399_003337"
     CDS             8499..8705
                     /locus_tag="PC399_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594165.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(8858..9286)
                     /locus_tag="PC399_003338"
     CDS             complement(8858..9286)
                     /locus_tag="PC399_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2594166.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(9762..10700)
                     /locus_tag="PC399_003339"
     CDS             complement(9762..10700)
                     /locus_tag="PC399_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2594167.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         3586 a         1916 c         1136 g         4142 t
ORIGIN      
        1 atgatgtaaa taatttaaaa ctactatatt atttaaaaat ttatatttat atatgcaatt
       61 ctaaaaactt aataagaagt gaacttagaa aataaagctt aatttcaaag gcttaaatag
      121 actattatta ttagtctaaa taaactacgt gtatcaaaat tatatattaa attcttctaa
      181 aatttttatt gcaaatcttg cgtttggata tgtaatagac cctccaccaa ttacacctat
      241 ttttatagct tctataattt catctttact tgctccagaa ttaatacaac cctcaatatg
      301 ataacaaata cactcatcac atcggcttaa aattgaaata gctagtccca ttaattcctt
      361 atttttttta cttatagccc cttctgaata agtcttatca tcaaggcttc caaatactct
      421 aaaaaactca tctgtttcac ttaatttttt attgtaaaat cccctatatt ctttcatttc
      481 tctagtattc atcttctacc tcctattttt ttcttataag agtatcacac tgtacttata
      541 ttttcaatat taaatacaaa ttttcatttt atgttcatta gcctaattta tattattttt
      601 tgaaatctca ataagttatc atataagtaa gattttttca gaactaaaat agaaatctca
      661 gtaatttctt tattatctat ctcagttctg aaatatatta ttcttatcca aacattatta
      721 tagtgattaa ttatattagt tatttttacc tgaaatttta tacatcaaat aagaatatac
      781 aaatggtagt attacaatta caaagattac aaagatatta taaaaaggaa tattaataaa
      841 tgcattaata attaataata ttccaccaat aagccaaatc catccagcta aatggtgtgt
      901 cttattccaa ttatcttcat cattaagagt ccatggtaaa attattccta ctgagtaatt
      961 tcgtttacat ttaggaagat agtttccaaa taaaataact attattccta aaattaatcc
     1021 tgtataaaaa ttagactcca aactaactcc tgttgaatat aaaaccatca ttatttgtat
     1081 aattactgaa agtatagcaa ctccccattt tgataaattt tgtataacat ttgaaaaatt
     1141 agctttctta ggctcattct caagtctaaa ccatgtatac aaatggatac atagcattaa
     1201 caatggtaca ccaaaagctg ctataacctt tggagcataa ttatctggat ttccataatt
     1261 atcaaaatga attgcaactt catttggtaa tctattataa aaatatattg atacaataaa
     1321 tggtataaaa catattaaag tagataaaat aaattgttta tttatcgctt ttttcattat
     1381 ctttttctcc cttctccata aattgcgaaa accaaagcat aacctcttcg aaaactgaaa
     1441 tatttatttc gtagaaaata aagttcttat actttgtttc aaatatgagt cctgcctttt
     1501 ttagttgaga tagatgataa gaaattgtag cattcgtcat attaaatttt tcactaattt
     1561 ctcctgctga cattttattc tccctcaaca taaccaatat ttctcttctt atagggtctg
     1621 aaagagcttt gaatgtttct ggaaacccca acaccatcac ctcgctattt agaatttcat
     1681 ctaaatagaa tataacataa catccatttt atttcaataa ctatttagaa ttttttctaa
     1741 atagttattt ctaaatattt attgaaaatt ttcatattaa gattctcaat ttacttaagt
     1801 cgcttttatt taatcttact ttatctaatt tttttaactt aacaaaagtg tattgcacca
     1861 taattgttaa agcaaaaacc taatgactga ggtacttata tatataacaa tacttttaat
     1921 tttaaattaa aagtagctaa acccaattta aaaagtagtt aagtcctatt taaatagaaa
     1981 agaatgaaac gagaaatata ctgtataaaa gtacataaaa acatctacta attagataca
     2041 actataacag aatatattta ttattataat aacaaaagaa taaaaaaaaa ttaatttagc
     2101 atagccttgt taaatataga tttgaatatg aatttaaagt tatgtaaaat taaaaataca
     2161 ggaatgaaaa ttccattcct gtataaaatc taacttttga gtctcagtac aatcttattt
     2221 tgagacaatc ttttttgttt tatattttct tatatttaaa atataacgca tatcttatgc
     2281 aacatattca tatttttctt tatattcttt catcgcatca ttccaagcgt cttgtactgt
     2341 tacacctctt ttattagcaa ttttttgtgc cagttcaatt atcttcatag ctcttcttga
     2401 tatgttcata aacattcctc ctacaaatta ccatatattt catatatata aatcaaggtt
     2461 atatttttat gatatataat ccttctattg taagattaca gtttatatgt gtattatata
     2521 tgaactatat tagaagataa ttagaatttt tataaagcta tatgattaga ttaaatataa
     2581 aagctttaca attacttata aatttaaaaa gtctataaac taaactaatg tttgatatac
     2641 caattttacg accataaata atgccattac taaaaatatt ggttttatca tctttgaacc
     2701 tttctttata gcaactttag cacctacata agctcccatt atcatcacta atgcaaacac
     2761 tatagcaatt ttataatcta cttgaccatt tatcatgaat aataacaatg aagttatatt
     2821 acttgttaag tttaatattt ttgtatttgc agaagcatgt aaaaaatcat atccatatat
     2881 ttttataaat ccaaaagtca aaaaagttcc tgtacctgga ccaaaaaaac catcataaaa
     2941 acccattata agagccataa gcatccctag tcttaaattt ttcttattaa cagcttcaaa
     3001 attatcttct tgacctaaat ttttagaaat aaaggtgtaa atagccacta ttaaaatcat
     3061 agccacaacc aaaacattta atatctcttc acttattgat aatacacatc tcacacctag
     3121 aacactacca ataatagtaa atggtactaa tttttttaaa aggttgttat taatttttcc
     3181 agactttgca tatctatagg cactacttat acatcctgct gatgctgcaa atttatttgt
     3241 accaatggca agatgtactg gcactccaat tgccataaga acaggcatgc ttataagtcc
     3301 tcctcctcct gctatagaat ctacaaaagc agctaaaaac ccccctatac ataaaaaaat
     3361 aactgaaaac ataaaattcc tccatcattc tctttcaatt tttcttacaa tatatattat
     3421 ataatcttat ttaatactta ttatccagca tattctttct atattcattt ggagttatac
     3481 tattaaactt cttaaatatt tgtgaaaaat aactactgtt attaaaacct actttcttac
     3541 aaatatcact aacactcatg cttctatcta acagatattt tttagattct tctatcctaa
     3601 gaagatttat atactcagtt attgaaactt tatttctttc attaaaaata gaactcaaat
     3661 aatttggact tataaaaaaa acatctgata tactattcaa atttatattt tctttataat
     3721 atttttttat atattcgttt atattttcaa ttagatttcc tttatctttt ctccaaaatg
     3781 ctctattctt atcaatacac tctttggaaa attcaaataa tacattccat aaatcttcat
     3841 cactgtttac aagtgatatt tttttattat atttatttct taaataagat ataagtttta
     3901 aatcctctaa atctctaagc actgttctag acaatacaaa tatcatttcc tgaatagtat
     3961 ctctaacatc attatatctt aatttgtttg tatctattag attataaaca ccttctaaat
     4021 tcatattttt aatttctttt tcaatataaa gttctaattc aaagggatat tcttttgaag
     4081 aattattgaa catatctatc ggattaataa tatactcttc ttctaataat ctactatttg
     4141 cttttttaag tgcctcttca agtcttatat tttcttttct actcttcaat atctcctcat
     4201 cttttatact aagttgattt tcaaaaaaat tcctcttagc tatttcctga ataaattcag
     4261 acatcattaa taaattttct tttaatacat ataatctact tttaggattt aatggaaatt
     4321 cattgtaaat tttgtttatg tattttatat caataccaga actacttgat aattcagata
     4381 aatttttatc tatataaata gacttatata ctataaaatt accacattct atatatccaa
     4441 ctatattttc ttctaatact ataggtatct ctaaactgga aacaccatac tcacaatgat
     4501 acactttttc aaagttatca ttatttatgc ttttaacaag ttttttagta cacggtttta
     4561 tatttttaca aatatcgcaa aatttatttt ctttcttagt ctcaaatatt tctttatact
     4621 ttaagtcata tacagatatt ctaagcttca atatattaga tatttgattt gcaagatttt
     4681 caattatgtt tacattaact aactcctgta tattgttttc agaaaatact ataggtattt
     4741 gtctcaaact cgaaaattta tgaggaacat aagtaattaa tattactaaa tcaattttac
     4801 cagtgtttat aatttcatgt tctgaatatg gtgggcaata aactatagat gtttccttta
     4861 tattgacctt tttaccatca attatttgta ttccttctcc ctgaatcaca tataaaagct
     4921 gttcttcact taaatgaaaa tgcttttctt ggcagtttcc cggaaaaaat cttacaattc
     4981 cagcactaag tctattgaat tgtgtttttg atggttcgtg tatccacata acctctcccc
     5041 aatcaaattt ttgtacttca ttatttgact caataaaatc caccataaac tcacctcgta
     5101 gtttttgtct aaatattttt tcttcccttc ttatattaaa ataatatcat catcattaac
     5161 tttcattact ataaaatcat catactcttt taatactcct gtaattttag ttgcacttag
     5221 tttactacaa ttcagttttt taatatattg ataaattcta taattacttt tttattttgt
     5281 aaaatcagtg ttttcccaaa ataattatat ctataaatga aagtattttt actgcttaca
     5341 tcaatatatg ttatcataat tttccttttt attgcatata taaataactg tatataacat
     5401 tgtccaatac tatatagaaa tacatctaag aaagaatatt attaaattgt tccaattaat
     5461 attatttttc ttatagaaat aaaaaactgt tggattcttt tagaaaaatt attttttaca
     5521 tacctttttt gcttaaatag tctatcttct cagctatttt gtatatacac tcttctagtt
     5581 catgatttta ttttaatatc ttgcttatat tgctgaaata attaaattct tttatctatc
     5641 atattgtctc ttatatcaca aacatattgt ttcttatatc acaaaaatga aagcattctt
     5701 atatcttcaa tatataaacc tgcttttttt aatcttgtaa taaatatagg atactctata
     5761 ttctcttcct agaaataact aataccactt atttttctat catatagaaa gtaataaatc
     5821 tttttcttaa taaaattcaa tagtaagtgg aataaaattt tatatgaata agaacattat
     5881 aaaaatactt tatacaatag tcaataaaag atattagctt caaattgtgt tgaagttgta
     5941 ttatttaaag tggaaaagca aataaaaaag aaataatggt ataagtagaa aaatatactt
     6001 aaaacaaggc gaagcctctc ttgtatctaa atcaagtaga gaattaaatc taaaaaagaa
     6061 aataaatata aaagatgact cctgtacatt acaagaatca tcttatggtt tcataatcac
     6121 tccatggttt ttaactgtct gatataaagg tctcagttca ctcatagcat gaccaccttt
     6181 tccgattgct gaccccgtca cttatggaca aaatgtccat aagtgatagt cctctcttaa
     6241 aaccctctaa atagatatat ctcaagcacc tcattgaaac cagttgctgg aaacaaactg
     6301 aagcgttctg gaatagtaaa agaaattgcc accatatcca cgttactgtc ctagttgtta
     6361 ctttgctaac ttcggagcga tttcctctaa caatacgcag tcaaattctg cctttttgct
     6421 aagaaatggc agatggtctg catctggtat aaaaaaaatc tgtttgtctg gcgccactat
     6481 ttcatcaaaa aatttcttcg ctggaagata ggggctatta tagtcatttt ctcccatgat
     6541 gtagtataca gggacctgat agtcaactcc ataatttctt atgtcagctt cttccactaa
     6601 aaaccgcaat acatcacctt gattgtcaat cgcatttgga ttgacaatgg ccgtattttt
     6661 taactctctc aatgaataat atggggttgc cagtatactg attatattgc ccaaatccat
     6721 atttatagaa agcccatact tgctttggta tgttcttact gtcataagag ctgccaaata
     6781 gcttggacca tattttgctg gcggatatgg tgcaatagct tccagcgcct tgatatcttt
     6841 ttgcttccct gcagctttca ccatttcaag tgtcttttca tagcccacgc gctcattgtc
     6901 caccatatta acagtctgcg caatcccaat gtacgcgctg taatcctccg gataggtttg
     6961 cacagccata gttcccagca ctgttcccca gctatgacct ataaggataa ttttctcctt
     7021 gttgaactct tgcttcaaat atgctgtcac ctcatggacg tcttgcagaa cacgctccgc
     7081 gctcatggtc tgcaaaactg ctgccggatc gttcgcaaag tatgttttgc ctacgtttcg
     7141 ctgatcccag ttcaccacag taaaatcatt ttcccactca tactggtaca gatgtaaaaa
     7201 tggcatcatt gaattaccag gacccccatg aacccaaagg atgatggggt tgtctgcgtt
     7261 ttgtccccgg atatataacg cctgctgtat gccgccgatc tccaccattt ccatgcggtc
     7321 gattccagga gcttctattt tatggcttgt acgtataatc gtgggggcta tcagacgcaa
     7381 accggcaaac gccagaacaa aaacagcaac tagtatgcaa agtgtgatta atatcatttt
     7441 aagtaccttc tttcatgaat ttttttagcc atagatatct cctctaacat taaaaaaagt
     7501 atgttgctat tttagttttg ttactctacc tgtcccattg gtttcctttc ataaaaaata
     7561 ttaattgatt ctataatctc atcttccagg atatcaactc ctatttgtct cataagaaac
     7621 tgtaagaatt gaaaccgttt acgaagtgta ggaaagtccc cctgaaatac atctgtatca
     7681 caccaaaagt taaagagcag caaaattacc tctgcgcatt catctgggaa ctctgtgatg
     7741 atgcttctat cctctatgcc ttctcgtaaa acatttgcta atattggtgc tagttttttc
     7801 aaattatcct gtgtaaatgc cagtatgata tgcggacttt ctatcgcgct tgtaatcatg
     7861 ttactagatt cttttatcac ctcatccgtt aaatgccgcc taactaaact tctaagcttc
     7921 tcttttgctg taagtccatg catttcaccg agccattgat ttactgtctc tattatctgt
     7981 tcaaagcgcc tttccatgac cacattaaaa atatcttctt ttgaactgaa gtgatgaaaa
     8041 attccgccct ttgacattcc cgaagcatca acaatatttt gcatacttgt tttgtcgtat
     8101 cctttttcag caaacaactt tgcagaaatg gtaatgatat tttcaatagc ttgctttgga
     8161 ttgttctttc tagtcattat ataactcctt cttattaaaa accgactgtt ggtttttatt
     8221 atataagcta ttcctgcttc tgtcaagtaa cattgtgttt atggcaaaac aataaggttt
     8281 ggtggttaga caggtattaa ttagactaat gcgactttcc cctagatgga acccttggta
     8341 aatttgcaat ctttaccggc tttgattata tctgtaagca aggcaggtat ggccacgcct
     8401 gccctgtttg accctcggat attcattcct atccctcctc tccttagcca ttacaccaaa
     8461 acgccgctca ttgtctccaa tggcggcgtt ttaaagatat gatttctcat gctgctgaaa
     8521 tatgcaaaaa atataagatg ccctttatta tttttcctac aatagaaaag aaaagtattg
     8581 aagaatgggt taatattgga gcaaatatgc tttgttttgc acaagatact acctttatca
     8641 cttatgttat caatcaatta ttatcttttt ataatgagat tgtaccaatt caaaaacaac
     8701 attaaattat aaatctaatt ttatattaaa agagactaat attttatcta tctctattaa
     8761 ggatattttc ttatgtatca taatattagt ctctcttatt attatatact gctatcatca
     8821 ttagttgtta tttatactat ttttataata tacaaattta ttcagctaac gttttgccta
     8881 attctataca ctcatctttt gcttcttcat ttgggtcttc cattgctgta attccttctc
     8941 caccaataat tgtagcacca gcattttgca tacgttcttc ccagtctctc atccattcac
     9001 aatttcccca tccatatgaa ccaaataatc caatctgttt acctgatacc atagattcta
     9061 attctgaaac aaatggctcc atatacccct cttctagttg ctctgctccc atagatgggc
     9121 aacctaatgc aaatttatct tcttctttta aatcacttgg tttcagtaag ctcacatcta
     9181 aaacttctgg tgtcttacct tttaacttca caccttcagc cacaaaattt gccatttttt
     9241 cagtatttcc tgttccactc caataaacaa tatatatttt actcattttt acctcctgta
     9301 gtatttttta attaatcaat agttagttat agctaactaa catctaaaaa acaaaagatt
     9361 tctttatctg aaatctcttt atttttctaa atatagaata tcatagttga tattgaaatt
     9421 caatacttat tgataatatt tatcatttat attttatatt cattttataa tatcaaaaca
     9481 ctgtatcgta tttctaatct ttcatagaaa aatttctaac ttatagtact tttgcatctc
     9541 ttataaatct tttatatgta taagtacata gaaaaacaca ccttttccag atgtgctatc
     9601 tataatatcg tatatttatt tttcaattct aactagctaa tatgtttata aattttatca
     9661 cttaatacct ttatacattg atgtaaaatc ttacacctaa gtaattttta ttatttgtct
     9721 cgactctata taatatagct attttatcat caaatcaaaa attatctttt actattattc
     9781 ttgtatttat agagatttaa atataagtat ccatttcctt gttccgttct aggtattcca
     9841 agcgctctcg tacattttat taattgtgca tacagttcag cttcatctaa atctctttca
     9901 aactcctctc ttgcccattc tttaattagt gctaatgagc ctgatacgta tggtgcactc
     9961 atactagtac cactcataat agcaagttta ttatccatat aagttgatat aatatttctt
    10021 cctggagcta ccaaatctat tgtagtattt gaattactaa acttttcaac caaatagttc
    10081 tcatttattg cacctacttc tattacctca gcataactgg ctggataact atactcactt
    10141 gtactagaat caccatctcc attattacct gctgcacaca ctacagaaat attatttttt
    10201 actgcttgca tgacagcatt ttttaaattc ttatcatctt tgtttccccc aagagacata
    10261 gatataatat caaccttgtt atttacagca aagttaatag cattaattat actttgatat
    10321 gttccggtac catctttatt taatgctttt gctattaata atttacaatc tggagctact
    10381 cccataactt cattattata attagatgca gctataatac ccgccacatg agttccatga
    10441 ccattaaaat cctcatatat atttttattt ccattactgt catcactaaa atttgcacca
    10501 ccaattattt ttccttttaa aagaggatga gatatatcac aacctgtatc tattatacca
    10561 actacaatat ttttaccagt ataaccttca tcccacattc ccctagcatt catttgtttt
    10621 attccatatg ggaatttatt ttttgcacct cttagatttt catttatttc atatggaatt
    10681 agtttcattc ttttattcat catatatcct cctaaaaatt ttattgctta taaaaatcat
    10741 gaattttatt ttataatagg tttataaatc aaattaaaac
//
LOCUS       DAMJWS010000113        10373 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMJWS010000113 DAMJWS010000000
VERSION     DAMJWS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.118"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            200..1624
                     /locus_tag="PC399_003340"
     CDS             200..1624
                     /locus_tag="PC399_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF2594168.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANESEEDSTSLEEAKAKMSDMKSASKNSEKNIEKKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLDIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1659..3788
                     /locus_tag="PC399_003341"
     CDS             1659..3788
                     /locus_tag="PC399_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2594169.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFSLASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIIK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKGKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNTKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIMSIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDE"
     gene            3818..4240
                     /locus_tag="PC399_003342"
     CDS             3818..4240
                     /locus_tag="PC399_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2594170.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAK"
     gene            4259..5410
                     /locus_tag="PC399_003343"
     CDS             4259..5410
                     /locus_tag="PC399_003343"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2594171.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEIYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSNGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5516..5617
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5800..6372
                     /locus_tag="PC399_003344"
     CDS             5800..6372
                     /locus_tag="PC399_003344"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF2594172.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKEEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6436..7086
                     /gene="fsa"
                     /locus_tag="PC399_003345"
     CDS             6436..7086
                     /gene="fsa"
                     /locus_tag="PC399_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2594173.1"
                     /translation="MIFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHEGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7238..10162
                     /locus_tag="PC399_003346"
     CDS             7238..10162
                     /locus_tag="PC399_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2594174.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKEPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPVLNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4254 a         1009 c         1951 g         3159 t
ORIGIN      
        1 agtattattt acaaaatcat atactccaaa atactacata aataaaatta aacaaaaact
       61 aaaatgacaa atgtcattat ctaaagtgac atagaaacgg tttcttaaag ttgttactat
      121 aaaatataaa ctctataata aaagtataac aaaagggaat catatcgata tagaatataa
      181 ttcaagagga ggtaaaatta tgagtagttc taatcaatcg acaataaaga catctataca
      241 gcgagtaggg aaatttttaa gtggtatggt aatgccaaat ataggagctt ttatagcatg
      301 gggtcttata acagcattat ttataccaac aggatggatg cctaatgaaa aattgagtac
      361 tataggcgac ccaatgataa aatacctttt accactattg atagcttata ctggtggtaa
      421 agcaatagca ggacaaagag gtggcgtaat aggtgcagca gcagctatgg gagtaattgt
      481 tggggcagat ataccaatgt ttatcggagc aatgataatg ggaccatttg caggatgggt
      541 aattaagaag ttcgataaat ttgtagatgg aaagatacca acaggatttg aaatgttagt
      601 aaataacttt tcaataggta taataggtat gcttcttgca atactaggat tttatgcaat
      661 tggaccagca atagtagcag ggacagctct tatagaatct ggagttcaat ttatagttag
      721 caaaagttta ttgcctttag tttcagtatt tatagaacct ggaaaagtat tattcttaaa
      781 caatgcaata aatcatggta tattagggcc tataggtata gctgaggcaa aagaagcagg
      841 taaatctata atgttcttac tagaaagtaa ccctggacca ggtttaggag ttctattagc
      901 atattggatg tttagtagag gaagtgtaaa acaatcagca ccaggagctg taataataca
      961 tttctttggt ggtatccatg aaatttactt cccatatatt ttaatgaatc cagttttaat
     1021 attggctaca atggcaggtg gagcagcagg aatattgact ttttcaatat taggagctgg
     1081 gcttgtagca gcaccatcac caggaagtat atttgcactt atggcactag ctccaaaggg
     1141 aggattactt ccagtattag caggggttgc agtggcaaca gttgtatcat tcttagtggc
     1201 agcaccattt gttaagagag cttcagcaaa tgaaagtgaa gaagacagta cttctttaga
     1261 agaagctaaa gcaaaaatga gtgatatgaa atctgcaagt aagaactctg agaaaaatat
     1321 tgagaaaaaa cagcttgaag taaatgagat taagaagatt gtatttgcat gtgatgcagg
     1381 aatgggttcg agtgcaatgg gagcttctag atttaaaaat agaataaaaa acttagattt
     1441 agatatagaa attacaaatt catctgttga taatcttcca gatgatacac aaatagtagt
     1501 aactcacaat acattagttg aaagagttgc taagaacaat tcaagtgttg agatagtttc
     1561 aataaacaat tttttaaatg acccaaattt agatacatta tttaaaagat tagaatctaa
     1621 ataaaaataa aaagtaccac taattaggag tggataattt gaaaaaaaag aagattacat
     1681 ctaggcaaaa aaagataata ctaatgatag tagaaaattc aaagaaaaac attccaataa
     1741 ctatttctga gattgcaggg acgttagagt taagttcaag aactgtactt agagatatgt
     1801 ctggaattga aaaatggttt gatgaaaatg attttaactt tgtaaaaaaa ccaggagtag
     1861 gattgatttt agaggaaaat atagaaaatc aaaactttat aattgagttg ttagaagaag
     1921 aaaaaataga aaaagaatac agcaaggaag aaagaaattt aattatatta tcaaagctac
     1981 tagtttctaa cgagccagta aaatcttatt attttgcaaa aatattaaag gtatctgaag
     2041 gggtactgaa taatgatttt tcattagcaa gtaaatggtt ggaaagattt gatattgagc
     2101 ttgttagaaa accagggctt ggtgtctacc taaaaggtca agaaaaaagc tttagagagg
     2161 catatgtaaa tcttatatac gattcattca atgaaaaaga aatactagat atggtcagaa
     2221 atataagtga aaatatacaa acagataaag caatagaaat tttaagtgaa aatagacttt
     2281 taaatttaat ggataggtgc ataataataa aagttgaagc tacattaaca aagaagcttt
     2341 cagatttaga tgtaaattta gctgatagtg catatatagg gcttgtagtt catatttcat
     2401 tagccttaca gaggataaaa aatggtgaaa atataactat ggataaagag tttctaaaag
     2461 aattatctat aacagaagag tttaaattag ctaaagaaat cgtaaaaggt atggaaacag
     2521 attttaacat ggatatacca gtagatgagg ttggatatat aactatgcat ataagaggtg
     2581 caaagcaaag gtcatcttca aatcataagg ctttaaactt ggatgatata gaaataatgg
     2641 aaattacaaa taagatgata gatttggctg aagatgaatt taaaataagt ttaaaaaatg
     2701 acgagaggtt atttaaagac ctagcaaatc acttaggacc ttctataaat agattgaata
     2761 tgggattaga aataagaaat cctcttttag atgaaataaa aagcaagtat tcatatgcct
     2821 atgatggagt tgaaaaaatt tctagaatta taaaagataa actaaatata aattcaatac
     2881 ctgaatcaga aataggatat attgcaatgc actttgcatc agccatagaa aaaaatctta
     2941 tgatgaatac taatataaat attgttgtgg catgtcccac tggaataggt acttcaagat
     3001 tcttatcaac taagattgaa aataaatttc ctaatttaaa tatacttgaa accatatcag
     3061 ctataaacat agatgaagag tatttaaagg gaaaagatgt tgatttaata gtgtctacag
     3121 ttgagttaaa tacaagttta aattatatat gtgtaggtcc atttatgagt ttagatgatg
     3181 aacagataat caaggaaaaa ataaaatcta tagctcaaaa taaattaatt aatttaaata
     3241 caaaaaatga tactaaaagt aagaataaag tatatgaaca gataacagaa agtatgaata
     3301 taggaaaaga tattcttcaa tttttagagg aaattagatt tgaaaaattt gaaagtaaag
     3361 atttaagtga gcttattgaa gactcatcaa aaatatttgc aaaatctagt gaagatataa
     3421 tgtctataaa agaatcgtta aaagaaagat taaaaatctc aattccatat atagaagaat
     3481 caaaaattct attattacat tgtatgagtg aaagaataga tattatgaag ttatcaataa
     3541 taaaactgga gaatcgaatc gtgctagatt caaatgaaga aatcgacaat gtagttttta
     3601 tgttacttcc taaaaattca ccaagttatc aaagacaaat aatgagtgaa ataagtggtt
     3661 cattaataga caatataatt tttactaata agataaataa attctctata gaagagatga
     3721 cattagaggt aaaagatata gtttttaatt tttatacaaa cagattaaaa gcatttatag
     3781 atgaataaaa ttaatattaa aaaggaaggt tgatactatg agtaaagtat tgaatgagaa
     3841 taatatattt ttagggttag atagtgtttc aaaggaagaa gctataactt tagcaggaag
     3901 aaaattagtt gaaaatggat atgtaaaaga agaatatata ccagcaatgc tagaaagaga
     3961 aaaagtaatg actacatata tgggaatggg agttgctatt ccacatggtg taaatgaagc
     4021 aaaaaaagaa atattatctt ctggaatagt tatactacaa ttccctaatg gtatagattt
     4081 tgatggagaa aaagcatact tattaatagg aatagctggt gttggagatg aacatttaga
     4141 aatactatct aatatagcag tagttttaga tgatgactta acagaaagac taaaaaattc
     4201 aaatgacaaa caagctttta tggaagcttt tgccaaataa tcttaggagg gatttaaaat
     4261 gaaaaaggca atccagtttg gagcaggaaa tattggaaga ggttttattg gaggcttgtt
     4321 agttaagtcg ggttatcatg tagtatttgc tgatgtaaat gaggagatat taaactcaat
     4381 aaataaagat aaaaagtata ctatacacat aagagatgta gaatgtatag atgaagtaat
     4441 tgataatata agtgcggttt catctataaa agaagaaata atagatgaga ttgtacaggc
     4501 tgaaataata actacagcag ttggaccttt agttttgact aaaatagcct ctacaattgc
     4561 aaaaggtata aaggctagaa aagaaaaggg tttaacaagt aatctaaata taatagcctg
     4621 tgaaaatgca atatacgcaa gttcttcttt aaaagaagag gtacttaagt atttaaataa
     4681 ggaagaagta gaatatttag agatatatgt tggttttcca aactgttcag ttgatagaat
     4741 agttcctcct ggtaaaaatg aaaatcctct tgatgtaaca gttgaaaatt tttatgagtg
     4801 gaatgttgaa aaacaaggat ttaaagggga aataccaact atagttggaa tgaatttagc
     4861 tgacaacctt atggcttata tagagcgtaa attgtttaca ctgaatacag gtcatgctat
     4921 aacagcttat ataggatatt taaaaggata taagactata gaagagagta taaaagataa
     4981 atttatctgt gatatagtca aaagtgcaat ggtagaaagt ggagaaggat tgattaaaaa
     5041 atacaatttt gactcagaag cgcactataa atatattgat aaaattttaa atagatttaa
     5101 aaatccatat cttaatgatg atgtattaag agttggaagg gaaccattaa gaaaactaag
     5161 tgataaagat agattgatta aacctttaat gacagcaaaa tcatatggat tatcagttga
     5221 caatttgatt cttggaatag gtgcagcgct tcattataat aatagtgaag atactcaaag
     5281 tgtagaatta caagagttga taaagtctaa tggtgtaaaa aaagctgttg ctaaaatagc
     5341 taatataagc aatgacgaag aacttttaaa taatatcgaa aaaagttata tatttatgaa
     5401 aaatttataa atttacttga aaaaaagtag ttctgataaa gtaactataa tatattattc
     5461 tacacagttt gaattatttg ttgaataata gagaattata atatataatt aaaaggaata
     5521 aattaatata tttacctgta tatgtttgat aatatggttc aaatgtttct actaagctac
     5581 cattaatagc ttgactacag gagtgaagaa gatatagttt atacttattt taaattatat
     5641 attaagttaa ttactttgaa ttttaagctc ctgtctatga tatagacagg agctttttta
     5701 gtattttaat ataaaatact tacaagaata gatagcaaaa ttagaacaaa tataattttt
     5761 ttattaatat aactttagaa agggctaggt ggaagttcaa tgaaggcttt acaagaaaag
     5821 atattgagag aaggcagtgt ttcaggaaat gatatactaa aagttgatag ttttttaaac
     5881 catcaaattg atgtagcttt tttaaatgaa ataggaagag aatttaaaga gagatttaaa
     5941 gaagaaaagg tggataagat atttacaata gaggcttcag gaatagctat agcatcaatt
     6001 gtttcacagt attttgataa tgcacctgta gtatttgcaa aaaaatcaga atctaaaaat
     6061 ttggataaag atgtatatga gactaatgta tattcattta ctaaggcaag agaatattca
     6121 gttaaagttt ctaaaaagta cataaacaaa ggtgaaaata tattgatagt ggatgatttt
     6181 ttggcaaatg gaagagctgc tcttggactt aaagatttaa ttgagcaagc agaagctaat
     6241 ttagttggag taggaattgt cattgaaaaa ggatttcaag ctggaggagc acttttaaag
     6301 gctaatgatg taagattaga gtctttggcg gtagttgaat ctatagataa tggaactgta
     6361 aaatttaggt aatttatgat attatatact tgtgtttagc acatatttta actatgtaat
     6421 tatggaggat tttttatgat attttttata gacacagcca atattgaaga aataaaagaa
     6481 gcaaatgatt taggtgtaat atgtggtgta actactaatc catctctaat agctaaagaa
     6541 ggtagagatt ttatagaggt agttaaagaa ataagtgaaa tagtagatgg accaataagt
     6601 gcagaagtaa taagtttaga gcatgaaggt atgatagagg aagcagaaaa attatctaag
     6661 atacataaaa atatagttat aaagatacct atgacagcag aaggtctaaa agcggtgaaa
     6721 gtactatcaa gtaaaggtat aaaaactaat gtaacactta tattctctgc tggacaagct
     6781 cttttagctg ctagagctgg agctacatat gtaagtcctt ttgttggtag attggatgat
     6841 atatcacaaa atggtcttga tttaattgaa gaaatcgttg atatatttag tgtaaatagt
     6901 attgaggctc aaataatagt tgctagtgtt agaaatccaa tacatgtttt acaagctgct
     6961 agaatgggag cagatatagc aacagttcct cttaaagtta taaaccaaat gattaagcat
     7021 cctttgacag acaaaggaat agatagcttt atgaaggatt gggaaggtgc ttctttaaaa
     7081 ttataattat aaactacgct ttataagtat attaaagagg ttattatgga aattctaagt
     7141 aataaggcta ggctattttg cctagtctta ttttggtgta taatatattg aaacatatta
     7201 ttgaaataga ttaatttttt aagtgaggag gcgaaaaatg gataaaatat taagagagat
     7261 acaaagagaa gataaaaaaa atccatatac tgaccaagaa cttgctgaaa tgttaaatgt
     7321 agctaggtct gaagtgatat cagtgaggaa aaaaaataat attttagatt cgagagaaag
     7381 aagaaaaaaa atattaataa aagatatatc aaaaattgta agtgaaaatc ctcaaatgtc
     7441 tgaaagaaaa ataactgaga tattaataga gaatggatat aatatatcga gaagtgcagt
     7501 ttcaaaatta ttaaaagaag aaaatttaaa tcagtcaaag attgggatta aagataaaga
     7561 gccagcatca tcaggaaagt atgtgaaggt gagtaaagtt gtagaagaag atggatttga
     7621 agaattgata ggtataaagg gaagtttgaa agaaaaggta aatctagcta agtcagcaat
     7681 aatgtatcct cctaatggcc ttcatactat aatatatgga gaaacagggg taggtaaaag
     7741 tgaattagct acatgtatgt ataaatatgc tataaaaaac aacatcaaag aagagaacag
     7801 cccatttata gtgtttaact gtgcagacta tgcagaaaat cctaatctat tgatagctca
     7861 actctttggg gtagtaaaag gtgcatatac aggagctgat gcaaatagag aaggtcttgt
     7921 agaacaagct aataatggaa tattgttttt agatgaaatt catagattgc ctgctgctgg
     7981 gcaagaaatc ttattttcat taatagatag aggagaattt agaaggttag gagaaagtag
     8041 ttctaataga aaggcaaatg ttctcataat aagtgctaca acagaaaacc cagattctaa
     8101 tttacttcaa acatttagaa gacgtatacc aatggtaata aatttaccta atatgagtga
     8161 aagaccaaaa tctgaaagat atgaaataat aataaagttt tttgaaagag aagcaaagcg
     8221 agtaaacaag aattttataa taactaaaga agttatgtgt gcacttatga attataagtg
     8281 tactggaaat ataggtcagt taaagagtga tatacaagta acttgtgcta gagcttttag
     8341 taaatcaata ttttctagcg ataaggtaat tatagattta gatagtttaa gggattatat
     8401 aaaaagtgga tattatgatt gtaattgtaa aaatgaaaag tattctgagt tttcagttga
     8461 ggatatatat atagatatca gtgaattggt gagtggaaaa aatagaattt tatctgataa
     8521 tgagataagc gagatatata attatgcaga aaaagagctt aaggttttgg aaagtaagtg
     8581 tttttcagaa gaagagttaa aaaatgcatt tatagaaaag ttagataaga agtttaatgc
     8641 tattaaaaac aataaaaatt tatctgatag aagaagtcga atagactggg gaggacaatt
     8701 aaaagagtct acattggaga taatggataa ggtaattgta tttattaagg agcagtttaa
     8761 gcatgtaaat caaggattgt acttggctct agcaattcac attgagcatg ctataggtag
     8821 gataaaagat ggaaaaacta taataaatcc gagtttagat aaaataaaag catcaatgcc
     8881 aatggaatat gaattgtcaa gatatataat gggtatagtg gaagatttga cacaagtaac
     8941 atttccagaa gatgaacttg gatatttggc ttattatata aataaatttt gttataatga
     9001 ggaaagtata aaagataaag ttaaagtagt aatagttacg catggtaaag ttggaattga
     9061 aatgtctaaa gttgtaaatc atattttagg tatagaatgt acactaggta tagaaatagc
     9121 acttacagat tcaccatcag aggggataga gcatgtatta gaagagcttc aaaagataga
     9181 agctagaaaa ggtattctag ttctaattga tatgggttct ttggtaattc ttggtgatga
     9241 ggttgaaaaa agacttggaa ttagatgtaa aactgtaaac agagtagata cgttattggc
     9301 aatggaagca ggaaaacttg caaccattga aggaaaaagt ttagatggta taatagcaga
     9361 tttaaagaaa aataagaatt atgccatgat aaatacaaat aaatttagtt acagaaaaaa
     9421 cgagtatgga aagaaaaatg taatcattac tttatgtttg agtggtgtag gtacagcttt
     9481 aaatttaaaa gagcatatag aaaaacaaat taaggaatat gacactttaa ttgaggtaaa
     9541 gccagttgct tttttaaata ataatttaga agaagagtta aatgatattg aagctgaata
     9601 taatattatt gctatctgtg gaactatcga tatagattat aaaaacgtac cttttatttc
     9661 ttataatgag gtacttggta aaaatggtat taataaagta ttagagcatt taaacagtaa
     9721 acaaccagtt ttaaatattg aaaaccagtt gaataattta atacatgaag atttgatttt
     9781 atacaatttt gaaggaattt caaaggagta tataattgat actcttgttt caaagcttga
     9841 agaaggtgga tatgttgact caaagtatat attaagtgta tataaaagag aagcaatggg
     9901 aagtgttgta atggcctcta aggtagccgt gccacatgga cttccagaaa acgttataaa
     9961 gcctgcaata gcaatcgcaa ggttgaataa gcctatagtg tgggacaata aatttatggt
    10021 ggatttggta gtactactgg cacttaaaga aaataataag aaagaaatac gaagcttatt
    10081 ttcaaagatt aatgaccaac atactttaga agttttatta aatactgaag ataaaaatga
    10141 aataaaagaa cttttaagct aattattagt acagagaaga aagacaaaaa atttgcttta
    10201 gtataaaatt taaaactact taattgctaa gtagttaatt actcgatata aggctagttc
    10261 tatgaaattt atttatatga ttttatagaa ttagcttttt tcttggtaaa taaaatatat
    10321 gaatttattt atgcaaaaag taaattttat tggacaattc tatgagaatt ttt
//
LOCUS       DAMJWS010000114        10246 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMJWS010000114 DAMJWS010000000
VERSION     DAMJWS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.119"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..249
                     /locus_tag="PC399_003347"
     CDS             <1..249
                     /locus_tag="PC399_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2594175.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIRTIG
                     FGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSVYKK"
     gene            265..1848
                     /locus_tag="PC399_003348"
     CDS             265..1848
                     /locus_tag="PC399_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2594176.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1876..3204
                     /gene="ssnA"
                     /locus_tag="PC399_003349"
     CDS             1876..3204
                     /gene="ssnA"
                     /locus_tag="PC399_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF2594177.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3349..4701
                     /locus_tag="PC399_003350"
     CDS             3349..4701
                     /locus_tag="PC399_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2594178.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4893..6002
                     /locus_tag="PC399_003351"
     CDS             4893..6002
                     /locus_tag="PC399_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2594179.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6285..7676
                     /gene="hydA"
                     /locus_tag="PC399_003352"
     CDS             6285..7676
                     /gene="hydA"
                     /locus_tag="PC399_003352"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2594180.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7670..10231
                     /gene="xdh"
                     /locus_tag="PC399_003353"
     CDS             7670..10231
                     /gene="xdh"
                     /locus_tag="PC399_003353"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF2594181.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTEDALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEEDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENVVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
BASE COUNT         3964 a         1236 c         2137 g         2909 t
ORIGIN      
        1 gaagaagatg aatttgttca agcagcgtat aagggattaa aagaagctgg aatagattca
       61 gaaataactc aatactcatt ctgtacgaat ggaagtcact acgcaggtga ggctggaata
      121 agaactatag gatttggtcc ttctaaggaa aatcttgctc atacaataga tgagtatata
      181 gaattagacc aattattcaa aggtgcagaa ggatattatg gtatattaaa atcagtttat
      241 aaaaaataat taggagatgg ttttgtgaga actttaataa aacatgggct tatagtagat
      301 ggaaataaaa caccagcata tgaaggagat attttaatag aaaacgagaa aatattaaaa
      361 atatcacaag atttaaacga agaagctgac aaagttatag atgcaaaagg tagagttata
      421 tgtccaggtt tcatagatac tcatagccat tcagacctag ttattttagt taatccatat
      481 aatgaggtaa agattagaca gggaattaca acagaagtgt tgggccaaga tggtatatct
      541 atggcccctc tacctcaaga gcatatatct tcttggagaa aaaatttagc aggtctagat
      601 ggtgaaagtg atgaaattga ttggaaatat gaaacaacag agaattattt aaaaatgatg
      661 gattacaatg gggtaggttt aaatgaaact tatttagtac ctcatggaaa cgttagaatg
      721 gaagctatgg gacttgaaga tagacctgct actaaagaag aaattcaaaa aatgtgtgaa
      781 atcacagaga gagaattaaa agcaggagct ataggattgt ctactggact tatatatata
      841 ccttgtgcat actcattgac tgaagaaatt atagagatgt gtaaggtagt agcaaaatat
      901 gacggagtat ttgtagttca ccaaagaagt gaagcagata caattttaac ttcaatggaa
      961 gaaatcatag aaattggaaa acaatcagga gtaaaagttc atttttcaca ttttaaggtt
     1021 tgtggtaaag caaactggaa gtatatacca caagttatag agcttttaga aaaagcagaa
     1081 aaggaaggaa taagagtatc ttttgaccaa tatccatatg cagcaggaag tacaatgctt
     1141 ggagtagttt tacctccttg ggcacattct ggaggaactg ataagttaat agaaagatta
     1201 agtgatgaaa acgaaagagc aaaaatgaaa aaagatatag caaatggaat agaaggctgg
     1261 gataatttta tagaatttgc tggaatagac caaatatttg taacaagcgt aaaaacagaa
     1321 aagaataaag atacaattgg taagagctta ttagaaattg gaaaaatgag aggaaaagac
     1381 ccattagatg caacgtttga tttattaaaa gaagaagaaa atgcagttgg gatggtagat
     1441 ttctatggat tagaagaaca tataatagga ttcatgaaaa gagacgagca aaatgtgtgt
     1501 acagatggtc ttttagcagg taaaccacat ccaagagctt atggttcttt ccctaaaata
     1561 ctaggaagat atgttagaga gttgaatgtg ttaacaatcg aagaagctgt ttataaaatg
     1621 actaaaaagg cagctacaag ctttagtata aaagatagag gcgaattaaa agaaggatat
     1681 tttgcagata tagttatatt tgacaaggat acagttagtg gttgtgatga ttttatcaac
     1741 tctatgcagt accctacagg tatagattac gtaattataa atggaaattg tgttgttgaa
     1801 gaaggaaaat acaataaaat taaagcagga aaagtactta aaaattaaat cttagcttta
     1861 aggaggaata aatttatgat tttagtgggg aatgggaggc taataagcca agattcttta
     1921 aatccctata tggaagatgg atgtgtagta ataaatgata atctaattga agatataggt
     1981 actacagaag aaatgaaagc taaatatcca gtacatgaat ttatagatgc aggcaagaaa
     2041 ataatcatgc cgggtctaat aaattcacat atgcatatat acagctcatt tgctagagga
     2101 atggcagttc caggaaaacc ttctgagaac tttttagaga tactaaataa tttatggtgg
     2161 agacttgaca aacagcttac actggaagat acaaaataca gtgcatatgc aacgtatata
     2221 gaatgtataa aaaatggtgt aacaacagtt tatgaccatc atgcaagtcc aaatgcaatt
     2281 gaaggcagtt tgtttacaat atcagatgtt gcaaaggatt tgggaattag aacgtgtctt
     2341 tgttatgaag tgtctgatag agatggtgga aatacaatcc aacaaggtat caatgaaaac
     2401 gtaaacttta taaaatacgc tcaaaaagat gatacagaca tgataaaagg aatgtttggg
     2461 cttcatgctg catttacact atctgatgat acattaaata aatgtgcttc agagatggct
     2521 ggtcttgata caggttacca tgtacatgtt gctgaaggac tagcagactt acaatataat
     2581 gtagataaat atggaaaaag ggttttagaa agattcaatg attttggagt actaaaagaa
     2641 aaaacaatag cagttcattg tatatatata agtgcagaag aaatcagtct tttaaataaa
     2701 acaaatacat ttgtagttca taatccagaa tctaatatgg gaaatgcagt tggatgttct
     2761 cctgcaatag agttattaaa aaggggagtt acagtaggat taggaactga tggatatact
     2821 agcgatatgt ttgaatcatt aaaagttgcc aatataattc acaaacatca cttaaaagac
     2881 ccaagagttg gatttgaaga atcacaaaca atgttatttg ataacaatag aaaaatagct
     2941 aaagcttatt ataaaaatga cctaggagta cttaaaaaag gtgcttatgc agatgttata
     3001 gtagttgatt atataccaca tactccattg acagaaaata caataggggg acacactatt
     3061 tttggaatga gtggaagaag cgtagataca actattataa atggaaaggt acttatgaaa
     3121 gatagaaagt taataaacat agatgaagaa agtattttag caaaaagtag agaattaagt
     3181 caaaatctat ggaatagaat ttaaataatt gatattaata tacaagtaag aaacaagaat
     3241 ttagatgtta aaaacagagc ttaaacaaaa ataggaattt aaatgaagta tataaataaa
     3301 aaatatacgg aatagaatat taaattaaat atagaaggga gacccaaaat gtctcgagat
     3361 aaaagcccta aaatagctcc agtagatgaa aggttacctg tatccaaatc ttgggtattt
     3421 tcactacagc acgttttagc aatgtgtgca ggagcagtag cagttcctat gatgatagga
     3481 agtgctgcac gactagatca tgctagtatt gtttttttaa taaatgcttg cttgtttatg
     3541 gcaggtatag gaacactcat acaaagtgtt ggtataggaa atattgtagg tgctaaaata
     3601 ccagtaatag aaggaactag ttttgccgct gtggcaggta tgacagcaat agcctcatca
     3661 tatagtgatt cacaagttgc aatacagaca gtatttggct ctgtaatagt agctggattg
     3721 ttttgtatat tacttgctcc agtatttgaa aaattaataa gatttttccc tagtgtggtt
     3781 actggaacag ttgttatggt aataggtatt tcactatttc cagttgggat aaaatggata
     3841 actgacaaca aattggaagc ggttgaacca aactcattag cattagcttt agcagtattg
     3901 gcaataactt tactttcatt taaattctta aaaggaattt ggaatagtac agctatattg
     3961 gtaggtatag tacttggtac actactttcc atggcagttg gaatggcaga ttttagtgca
     4021 gtaaatggag ttggttggct taacttaaat gtacctttaa aatttggaat gcctagattt
     4081 gaaccatctg caatactatc attgtgtttg attatgttgg tacttatgac ggaatcagtt
     4141 ggtaatatga tagctattca tgaaatggta gacaaagaag taacaggtaa aaatattaaa
     4201 agaggtctat ttggagatgg aatatctaca gcattatctg gaatattcaa ttcatttcca
     4261 ataactccat ttggtcaaaa tacaggaata gtaggtctta caggtataaa atcaagattt
     4321 atagcagttt acgcaggggt tatattgcta gtacttggat tcttaccaaa atttgcagca
     4381 ataataggag caatacctaa gcctgttctt ggaggcgtag gatttgcaat gtttggaatg
     4441 gtaatggttg gaggagtaaa aactttaagc aaagtgaaat ttgatggaac taaaaatggg
     4501 gttatagtag cagttagtgt tggtttgtct atgataccac taacaaatcc tgatttctat
     4561 cataatttcc caacttgggt acaaacaata ttccatagtg gaataacaac aggtagttta
     4621 tcagcagtgc ttttaaacat attcttcaat gaactaggtt ctaaaaaaga aaaaaataat
     4681 aaagttgaag aagcaaaata aaaataaaac aagtaaaaat ctatattgac ttaaagtcat
     4741 taagcattaa taaaaataat tcagagatat taataattca acaaaactat cttatatttt
     4801 aaataatata aaaaagtaaa taaataacga atacaaataa aagaatacaa tctaaatatt
     4861 aaaaatattt aatttaaggg agagataata atatggctaa tttaaaagtt aatttatatg
     4921 gaatggaatt tttaaaccca attatgacag cggcaggacc tggagcaaaa gatggtgctc
     4981 tctgtcaaga ggcagcaaaa ggtggagcag gagctatatg tacaaaaact atatctgtat
     5041 taccagctga tgtaccaaga ccatgtatgg caaaaactaa tagtggtttt ttaaacacag
     5101 aattatggtc agagcttcca aaagagcaat ggatagagga agaatacaaa aaggtaaaag
     5161 aaacagggct tccagtaata gtaagtatgg gatattcagc agagcaaata gctgaggttg
     5221 ctcctttagt aaagccttat gcagatgcag ttgaattatc tactcattat gtaggtacag
     5281 acgttgcacc aatagtaaat gctttaaaag ctgcaaaagc tgttttagat gtaccagtat
     5341 ttatgaaaat gagtcctcat actgacattc aaactattgc aaaagctgtt gaagaagcag
     5401 gtgcagatgg acttgtaatg ataaactcat ttggaccatg tatggcaata gatgtaaata
     5461 caggataccc tataatggga agtaaagctg gatacggatg gttatcaggt gcaccaataa
     5521 gacctctagc aataagatgt atatatgaag cttctcaagt tgtaaatata ccaataatcg
     5581 gtgttggtgg aatcacttgt ggaaaagacg tggctgaaat gatgatggct ggagcaagtg
     5641 ctgttcaagt atgtacagaa gcaatcctta aagggccttc tatctatgga aaaattgcta
     5701 aagaattaaa tgaattttta gatgagaatg gatatgctga tgttaatgaa ataaaaggat
     5761 taactcataa gaaaatggca gctagagggt ttagaactca ttcagtagct ccagtagtgg
     5821 atgataacaa gtgtatcaaa tgtaagcaat gtgtaacaag ctgtgtatac gaagctttag
     5881 aagttacaga taagttgaac atagatgcag acaagtgttt tggatgtgga ttatgtgtaa
     5941 ctagatgtcc aaaaggagct ttaagcatct gctacaatca agtagaagct atgaatgagt
     6001 aataattata tagaattttg gtaattaact tagagtaata ggtattagga taataaaata
     6061 tatcagataa acaaacacgc attatgcatt aataatacag tctaaagtta ataattttat
     6121 tatattgatt taactaaata ttagtttagc tgaatattaa tttaattagg tattggttta
     6181 atcttaatat agatttacta cgtataagtg aactaactag attactttaa gttaattttt
     6241 tcatataaaa taaattgata cattacaagt aaggtggagt ctatatggga attgttttaa
     6301 aaggtgggaa agtagtaaca agtgaaaaaa cttactgtgc agatgtaaga atagaagatg
     6361 aaaaaatagt cgatataggt cttgatatat caaaagaaaa tgatgaaata gttgatgtta
     6421 gtgattctta tgttatacca ggaggtatag atacacacac acactttgac ttagatacag
     6481 gcgttactaa gacagcagat aactttgaga caggaacaaa agctgctata gtaggaggaa
     6541 ctactactat attggatttt gctacacaag ctaaaggatg ttctttgaaa gacggattag
     6601 aagaatggca tgaaaaagct aaggataaat catattcaga ttatggcttt catatggcta
     6661 taacagattg gaatgactcg gtttgtaatg aaatggagga tatggtgaaa gagggagtat
     6721 cttcatttaa actgtatatg gcgtataaag gaagccttca agttgatgat ggtgtaatct
     6781 ttgaagcatt gagaaaagca gaagagatag gagggattat tggtttccat tgtgaaaatg
     6841 gggacataat atgtgaactt gtggataaag caaagtcaga aaataattta tctccaaaat
     6901 atcatcaatt aactagacca gctataatgg aggcagaagc tacatcaaga cttatgaaga
     6961 tagctgaagt tgcaaactct aaagtatatg tagttcactt aagctgtaaa gaatccttag
     7021 aggaggtttt aaaagctaag caaagaggtg tggatgtaat agttgaaact tgtcctcaat
     7081 accttctatt aaatgatgaa ttatatgatt tagatggatt tgaaagtgct aagtatgtaa
     7141 tgtcaccacc tttaagaaat aaagaaaata atgaaatatt atggaatgct attttagata
     7201 atcaaataga cactatagga acagaccatt gttcttttaa ttataaggga cagaaggaac
     7261 ttgggataaa cgatttcagt aagataccaa atggttctcc aggtgtagaa cataggatga
     7321 gtcttttata cacatatgga gtctgtaaaa ataaaatatc tatcaacaag atggtagaag
     7381 taacatcaac taatgcagca aagttgtttg gaatgtatcc taataaagga actatagaaa
     7441 taggcagtga tgcagatata ttagtcttaa atccaaacaa aaaatccaaa atagaagcaa
     7501 aaaaacaatt acaaaatgta gattatacac cttatgaagg gtatgaagtg gattgtcaaa
     7561 tagaacatgt ttatttaaga ggaaaagaag tagttagaga tggagaatta atttgtaata
     7621 ctccaagtgg gagatatata aataggcgtc taacaaaggg ggctagagaa tggtagaatt
     7681 tatattaaat ggagagaaag ttgttgcaaa agaaggcaaa aaacttttag acttccttag
     7741 agaagatatg aatataactt cagttaaaaa tggatgttca gaaggtgctt gtggaacatg
     7801 tatggttata atagatggta aagctactaa agcatgtgta tttaaaactg ataaattagc
     7861 tgggaaaaac ataacaacta tagagggatt atcagagaga gaaagagatg tttttgcata
     7921 ttcttttgct agtcaaggag ctgtacaatg tggattttgt attccaggta tggtaattag
     7981 ttcaaaagca cttatagacg ttaataataa tcctactgaa gatgatataa agaaagcttt
     8041 aaaaggtaat atatgtagat gtacagggta tgtaaaaata attaaagctg tcgatttagc
     8101 agcaaagatt tttagagaaa atatagatgt gccaaaagta aactgtaaag gtctagtagg
     8161 tgaaaatctt cctagagttg atgcagttgg caaagcactt ggatattctg aatatgtaga
     8221 tgatatgaga attgaaggta tgatttatgg ttcagcagtt agagctaagt atcctagagc
     8281 tttagttaaa aaaatagata tagaagaagc aaaagcttat ccaggagttg ttgcagttct
     8341 aacagctaaa gatattccag gaactataaa ggtagggcac ttaaagaagg attgggatac
     8401 tttaatacca gaaggtgaaa taactagata cttaggagat ggaatagttt tagtagcagc
     8461 agaaactaga gaagcgttag aagaggcaaa aaagctagtt aagatagatt atgaggaatt
     8521 aacaccatta agtactacag aagatgcgct tagagaagat gctccaaaga tacacgagac
     8581 aggtaatata cttgttaagg aacatttagt aagaggaaac gctaaagaaa agatagaaaa
     8641 ttcaaaatat gttgtaacta aaagatatac tacacctgct acagaacacg catttttaga
     8701 gcctgaatgt gcagtagctg gtccttatga agaggatgga gtaataatct attcaacaga
     8761 ccaaggggtg tttgccactc aacatgaatg tgcagatatg ttagggctac catatgagaa
     8821 ggtaagagtt ataaacaaaa tggtaggagg aggatttggt ggaaaagaag acatgagtgt
     8881 tcaacaccat gcagccttac ttgcatacca tactaagaga tttgtaaaag tacagttaac
     8941 aagaaaagaa agtattattg ttcatccaaa acgacatgcc acagaaatgg aatttacgac
     9001 agcatgtgat gaaaatggat acttaacagg tatgaaggca acgataatat ctgacacagg
     9061 tgcatatgcc tctcttggag gaccagtgct acaaagactg tgtactcatg cagcaggacc
     9121 atataattat caagatattg atataacagg aacagcagta tatactaata accctcctgg
     9181 aggagcattt agaggatttg gagttacaca gagttgtttt gcaactgaat gtaatctaaa
     9241 tttacttgct gaaatggttg gaatatctcc ttgggagata agatataaaa atgcaataag
     9301 acctgggcaa gttttaccaa atggtcaatt tgcagatgaa ggaacagcaa tggcagagac
     9361 actagaagct gtaaaagaag agtatgaaaa gcatccacgt gcaggtatag cttgtgctat
     9421 gaaaaatagt ggggttggag ttggtatacc tgatgttgga agatgtagat tggtgataaa
     9481 agatggcaaa gtccacataa gaacaagcgc ttcttgtata ggtcaagggc ttggtactgt
     9541 tatgactcaa gtagtttgtg aaactgctag cctacttcct gaaaatgtag tgcatgaagt
     9601 tccagatact catttaacac cagactctgg taatactacg gcttcaagac agacagtatt
     9661 tacgggagaa gctactaagg ttgcgtcaaa taagttaaaa gaagctttag actctggcaa
     9721 aactttactt gatttagagg gagaagagtt ttatggagaa tatgaaagta ttacagataa
     9781 aatgggttgt gacaaagaaa atccagtaag tcatgttgct tatggatatg caacacaagt
     9841 tgtaattctg gatgaaaatg gaaaattgga aaaagtagta gcagcacatg atgttggaag
     9901 agctataaat ccaatcagtt tagagggtca aatagaaggt ggagtagtta tgggtcttgg
     9961 atatgctcta accgaagatt acccagtagt taactctgtt cctacagcta agtttggtac
    10021 attaggttta tttaaatcta cagatatacc agaaatacaa gctttaatag ttgaaaagaa
    10081 caaagattta ttggcttatg gggcaaaagg tgtaggagaa atttgtacag ttcctactgc
    10141 accagcagta caaaatgcct attatgtatt tgataaagaa tttagaactg aactaccact
    10201 acaaaatact ccatatagta aaaaaaagta acaaaaaaaa gaaata
//
LOCUS       DAMJWS010000115        10204 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMJWS010000115 DAMJWS010000000
VERSION     DAMJWS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.120"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..874
                     /locus_tag="PC399_003354"
     CDS             <1..874
                     /locus_tag="PC399_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2594182.1"
                     /translation="KEKYKYILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIY
                     IENENLSKLYSQNLYDKLRISKDELKKVNADLETYASSIEELAILKERNRISREIHDS
                     VGHSLSTTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENY
                     QSLFKIKELVKSFIKLTNINVKLTISKNTWNLSRNQSITLYRLIQESLSNSSRHGKAT
                     EIRIFITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTI
                     RASFPKFSGGDFFE"
     gene            867..1514
                     /locus_tag="PC399_003355"
     CDS             867..1514
                     /locus_tag="PC399_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2594183.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1643..2578
                     /locus_tag="PC399_003356"
     CDS             1643..2578
                     /locus_tag="PC399_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2594184.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIIEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2592..3689
                     /locus_tag="PC399_003357"
     CDS             2592..3689
                     /locus_tag="PC399_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594185.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQNSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3692..4756
                     /locus_tag="PC399_003358"
     CDS             3692..4756
                     /locus_tag="PC399_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594186.1"
                     /translation="MIRLTIANIKRYLKNHNLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNELDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            5000..5497
                     /locus_tag="PC399_003359"
     CDS             5000..5497
                     /locus_tag="PC399_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2594187.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5673..5921)
                     /locus_tag="PC399_003360"
     CDS             complement(5673..5921)
                     /locus_tag="PC399_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594188.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(5987..7003)
                     /locus_tag="PC399_003361"
     CDS             complement(5987..7003)
                     /locus_tag="PC399_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2594189.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7061..7828)
                     /locus_tag="PC399_003362"
     CDS             complement(7061..7828)
                     /locus_tag="PC399_003362"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF2594190.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7896..10085)
                     /locus_tag="PC399_003363"
     CDS             complement(7896..10085)
                     /locus_tag="PC399_003363"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2594191.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3552 a         1566 c         1183 g         3903 t
ORIGIN      
        1 aaaggaaaaa tataagtaca tattgttctc ttttgttata atatcttctc ttattattag
       61 tttagataaa aatatatatt tggcactgga gtcaagctct atattgctaa ttataactgt
      121 actttctata tatatagaaa atgagaattt atcaaaatta tacagtcaaa atctttatga
      181 taagcttaga atttctaaag atgaattaaa aaaagttaat gctgatttgg aaacttatgc
      241 aagttctata gaagaattag caattttaaa agaaagaaat cgaatatcaa gagaaataca
      301 tgatagtgta ggacattctc tttctacaac aataatacaa ttaaatgcta tagaaaaact
      361 cttaaaagac aaacctctaa tttatgactt agtacatgaa cttagagaat ttgtaactga
      421 aagttttcaa gatgtaagaa gggctatatc tgagttaaaa cctgttgaat atgaaaatta
      481 ccaaagttta tttaaaatca aagaattagt taaaagcttt attaagctta caaatattaa
      541 tgttaaatta actatttcaa aaaatacttg gaacttatcc agaaatcaat ctataacttt
      601 atatagactt atacaggaat ccctttccaa ctcatctagg catggaaagg caactgaaat
      661 cagaattttt attacattca atacttcaaa tcttataatt actattagtg ataatggtat
      721 aggatgtggc aatataaaaa aaggaaatgg acttaatagt atcaatgaga gaatatatga
      781 attaaatggt aaagttgaat ttgataattc taataatggt ttcacaatta gagcttcttt
      841 tcctaaattt tcagggggtg atttttttga ataaaattaa agttcttata gtagatgacg
      901 aaaaattaat tagaaaaggt cttaaaatta tattatcatc ttataatgat ttagaaattg
      961 taggagatgc ttctaatgga tatgaagctt tagaattttg taaaatgaat gatgtagaca
     1021 tagttcttat ggatatacga atgaaagtat gtgatggggt tctaggtaca aggcttataa
     1081 aggaatataa taattctata acattattaa ttttaacaac ttttaatgat gatgaatata
     1141 taaaagatgc tatgaagttt ggtgcttcag gatacctttt aaaagatagc tctgatgaag
     1201 tattgcatga agggattcgt tcttcatttt ttggcaatat tgtgcttgat aaaagtgtag
     1261 ctgaaaaaat catgacctct gaaaaaacgg ttaaacaaga gcatctttat gacatgtaca
     1321 atttaacaga aaaagaaata tctataatta gactaattgc aaatggactt aataataaag
     1381 aaataagtca agaactattc ttatctgaag gtacaataaa gaataatata actaatattt
     1441 tagctaaact agaacttcgt gacagaactc aactagcaat ctttgctttc aaaaataaaa
     1501 ttgtaataga ataaatctaa aaatataaag tgacttaagt cacttttttt agtgacttat
     1561 agtaatagaa ttataattca caattcttta ttataatatt atagcttttt aacttataca
     1621 aaattatttt ggagggatta ctatggatat agtcagagta aataatataa caaaaagatt
     1681 taatgataaa ttagtacttg ataacatcag cttttctgtt aaaaaaggtg agatatttgg
     1741 acttattggc ccaaatggtg caggtaaatc tacacttata aatataatta caaatcttat
     1801 gttaccaaat agtggtagta ttcaaatcaa tggtttagat ttatcaaaag attatataaa
     1861 agcaaagtct ataatagggt tagttccaca agaattagca attatagaaa ctcttactcc
     1921 ttttgataac ttagagtact ttggagcttt ctacggttta aagggaaaac tacttaaaga
     1981 aagaatcatt gaagcattag aagttacagg tcttactgaa gtcaaaaaga aaaaggtcaa
     2041 aaaattatct ggtggtatgc aaaggagatt aaatattgga atagcactct taaatcatcc
     2101 taaaatatta atattggatg aaccaactgt aggtgtagac ccccaatcaa gaaatcatat
     2161 tttcaatttt ataaaagata tatctaaaaa acatgaaact actgtaatat atacttccca
     2221 ttatatggaa gaagttgaac acctttgttc taaaattttt attatggatg aaggtaaaga
     2281 aattgctttt ggagataatg attatttaaa atcactcgta tctacaaata caaaattaat
     2341 tatagaaata aaaaatataa atgcccaact tatctttgat ttaaaaaata ctaaaggtgt
     2401 tatatctgtt ttagaaaata attcattatt agaattggat atagataaaa aattacaact
     2461 tacagatata ctctctgtta ttgacaaaaa caattcaaaa ataatgaaaa tttcttatga
     2521 agaacctagc ctagaagacg tatttttaaa tctaacagga aaaaatttaa gggactaatg
     2581 gaggtgattt tatgaaatta tacatgagtg taaaaactat gctaaagagt ttaaaaagct
     2641 cctttatact taatttaata tattttttag ctttgcctct gattttatct tggtttcttg
     2701 ggatggttac agaaagcatg tttcaaaacc ctataaaaac agaatctacc cccattgtta
     2761 tatatgacaa agataacaca agattatcaa atgatttgac aaagtatttg aaaaatgatt
     2821 tatcttatat attaacagtt aaaaaagatg attctaaggc agaattaaag cttactatac
     2881 ctaaaggcta tgagagtagt ttattaaatg aaaaatcaaa tactctaaac atagaaaaac
     2941 ttggaactcg tgatgatatt gcaatattgc ttcaagatat attagatact taccatgaaa
     3001 aattttattt aaataattct cagaaaataa gttcagaaga tttttctaaa ttatttaata
     3061 aaaattctat tgatacttct ataataaata acaatgttaa acagaactct tatgaatact
     3121 ttgctttagt ttcactagga tttcttgtaa taatttttat aatgaacaat attttgtcaa
     3181 attatataag tgaatcaaag ggactttcaa aaagacttta ttctatgcct ataactagag
     3241 tacaattctt aatttatgac tttgtaggtt tatggattta ttcatttata ttcttattat
     3301 tatatgtttt attctttaga atacttggaa taacatttaa aggtaatttt gccatattgc
     3361 tcctactttg tgctttatca tcttatttta tgacttcaat atctaccttt gtaaccagct
     3421 tttttagtaa aaaatacggt acagcaatag tttatgcatt actcttttta cagacaattt
     3481 ttggaggtat cttcagtatg ataagtgata catttactaa gcttacaagt ttatctccta
     3541 cctatttaat aggagaattg tttagtaatt atgaaacatt taaaactata gattcaatag
     3601 gagatttaat tttcacatgt ttaattatct ctacaatatt aattgcactt gctattgtaa
     3661 aggaaaaata taaatggagg gaggtttaat aatgattaga ttaactattg caaatataaa
     3721 aagatatcta aaaaatcata atcttttaat taatatggtt atgcttccta ttatactaat
     3781 tttttcactt aattttttta tcaacaactc aggaaatcaa tcaatgtact tttctccagt
     3841 agcaattgta tctgactctt caggaaagta tgaacataag ttaataaata gttctaaatt
     3901 aaaagaaaat tcatttagat taaatgaact agataaggct atgaatctat taaaaaataa
     3961 taaggtttct gctgtattcg ttttagataa aaatttttca aatagtatag ataaattaca
     4021 acgacctgta gttaaatgct ttaaaattga aaatggtggt ggtagtcttt gggcagaatc
     4081 acaaatagaa tcttttatta ctaaatcgtt aaaattgaaa gtggataaaa atatagatga
     4141 taaattgaca aagacaaata taattgataa caaaccagat aagaataaag gttcattttt
     4201 agtagtcttc ttgatatgct attttatgta tattaatgca gcacatcttg cttctgattt
     4261 atttacatta aaaaaatcaa atgtgctaaa aagacttatc tctacagata ataaagatat
     4321 aaaaataatt ttcagtatat ttttaggact attttttatt caatcaattg tatacacttt
     4381 tgctctatta tgtttttcaa ttatagaaga ctttaatttg agtctgaatg tattgatgat
     4441 tgtactagca aatagttttg tttccactgg atttgtattt tgggtagcta gagtatttaa
     4501 aaatgaaagt tctataagtc ttatatctac attttattct ttaataggtc tagctatatc
     4561 tatctctagt ctaataccat caatggataa attatccttt atgactaatc tttcaaagtt
     4621 cacaccattt tattggacta tagatgctat taaaaacaat ggtagtattt taataaatat
     4681 aatagctcta atattaattg gaattatatt tgtaactgca ggaagtctta aactaagaga
     4741 ttttgctaaa aactaataat tattaaatat aattagtatc tatttttata tagttgtaaa
     4801 agaatatttc aacatattaa attattttat cttatatttt tctctttagt ttcacacatc
     4861 accaagtcta aaccttatat tatatattaa tatttttgac ttgtcaagtt atttatgtta
     4921 taatattaat cattagataa ttaaagcatt gtacctaagt tgcaatatct aaaattcaat
     4981 ttggaggtat aactagcata tgtataaaat tggtgaaagt gtaatgtatc caaaagaggg
     5041 agcctgctct gtaaatgata ttgtaacaaa gaaaataaat catgagatgc aaaaatacta
     5101 tgaattaagt gtcattttta atagtaacct taaaatttcg attccagttc taaatgcaga
     5161 tagaattggc atacgtccag ttatggatgg aaatgatgtt gataatttta tccaatctat
     5221 taataaaaca gatggtgtat ggatttttga tagaaaagaa agacttaaac tgtaccaaga
     5281 taaatttcat tcaggagatg tttttgaaat tgtaaaatta atcaaaatgc ttatgataca
     5341 agacagttca aaacaattgt gtagcacaga taaggagttt ttaaataaag ctcaaaaatt
     5401 tgctttatca gaattagctg ctgctcaatg taaatcctat acaatggtat tagaggaaat
     5461 gaaaaaacat atattaaatt caaagaatac aaattaaacc agttttttaa actggtttta
     5521 ttttttatac taatcatcca tgctacttta gcttatttta aactgcctaa tctaatatta
     5581 ataaagttat ctcaattaga tagatgtatt ttattcaaat aaaaaaaact aagagtaaat
     5641 catattactg atttactctt agctaataaa atttaatctt cttcaagttc ttttttctct
     5701 tgtaataata cacttaattc atttgcttct aaaacttgtg cctctgcaat atcaagtaac
     5761 tcttgttgcc attccacaca tttatctttg taatcaaaag gtagcgttct gtcaactatg
     5821 tacttatcaa tacagtcctc cgcactttta attatttctt ttctttcatc aataaatttt
     5881 tctaattcta ctaaggcttc ttcataagct tttctactca tataaattcc ccctttgtac
     5941 aattattcta cattaaaatg atttttcctc ttttttgtat aaaattttat ttgtatataa
     6001 cttttccatc aatatctttt atagtttgac atccagttaa tatcatagaa ctacttagct
     6061 ctgattgaag tttatttaca taagtttcta caccatcagt tgcaccacca aaagaagcag
     6121 tcacaaaagg tcttcctatt aatactgcat ctgcacctaa acctatcatt tttagtacgt
     6181 ctaaaccagt tctcactcca ccatctgcaa gtattgtaac tttaccttta acagcatcag
     6241 caatttttgg taaaacttca catgctcctg gtgtacagtc aagcactctt cctccatgat
     6301 ttgatacaac tattgcatcc acacctgctt ctactgccat taaagcatct tcaacagtca
     6361 ttataccttt caatataaat ggtagtttag tagactttac taactccttt atttgtttaa
     6421 catttttagg aagtactggt tttccatgta atgataaagt tactagacca caggcatcta
     6481 tatcaactcc aactgcaaat gcaccagctt cttcagctaa tcttatcttt tttataatat
     6541 tttcattatc ccaaggttta ataaatacaa ttccagctcc attatacttt ttgactacat
     6601 ctaaattttc cattaaaaaa gcatctactg ccgtatcacc aaccattgca taaataccac
     6661 tattagcaca tcctgcaact actggttcta tgtaatctct ttcattaatt tttccaccca
     6721 tatttaaagt agttcctgtt actggagctg caaatatagg tgcactcatt ttctttccaa
     6781 acatctctat agatgtatca ggatttgata catcatgtat tactctcata tttactttta
     6841 ctttttctaa actcttgcta ttttctataa aagaagaacc acttccttta cctcccatgc
     6901 caggcacttc acctgcacaa gcaaccccat tacaaacctt acaaacttta caacttccat
     6961 ttaaattttc tctagcactt tttaataatt cattatattc catctttaat cccccattaa
     7021 ctatagtaat ttatattata tacattatat catattatat ttacagtata tttttcaaaa
     7081 aactttttct gtggatagga gttatgccat atttttcaat agcttcatag tgttctttag
     7141 ttccatatcc tttgtgagat ttaaacccat attctgggta tactctatca tattggtaca
     7201 ttatactatc cctagtaact ttagcaagta tacttgctgc tgctattgat atagattttg
     7261 aatctccttt cacaattgga ttttgagata tgtcaatacc tggtattgtt gctgcatcaa
     7321 ctaataaata atccggtgct ttttttaaac aatttatagc ctttttcatt gccatatatg
     7381 tagcatttaa tatattaaat tcatctattt cttcattgtt tactatacct attccataat
     7441 ctagtgcttc ttcttttatt acttcaaaaa gttcatctct ttttgcctca ctcagtttct
     7501 tagagtcatt tacaccttct attttggtat cttttttaaa tactacaaca gaagctacaa
     7561 caggtccagc aagtggtcct cttcccgcct catcaattcc tccaatatat aaataacctt
     7621 tatcatagcc ttcgttctca aatatattta tagtttccaa tctttcttct tcttttctaa
     7681 tattatccag cttttttgct agttttatag ctagaccttg tacagatttt ctttcatcaa
     7741 ctcttaacag ctctatatat tccatatatt tttctacttc taaagtctct attatttctt
     7801 taatctccct cacactttta tcctgcatac cattccctca tctttcgttt ttaaaaattt
     7861 aatttttaaa tatattaaaa ttttttaatt agagtttaat tgcatccact atagccacat
     7921 tttaaacata tactacatcc acctgtatgt gcaattcctt cacttccaca ctcaggacac
     7981 acaaattttt catcttcttc aaaattttta tttattattt ctttggcttt ttcatcttta
     8041 ttatcttttt cttttttatt atttttataa tattcttttt tactattttc ttttttatct
     8101 tctttattat tttcttcctt tttttcattt attaaaattc cactcctttt acatccatct
     8161 ctaaatattg ttactccttt aagaccagcc tcccatgcct taatatatat attgaaaaca
     8221 tcttcaactg tagtttcaaa aggtatattt attgtagatg atatagaagc atcaatatac
     8281 ttttgccata ctttttgcat gtttattctt tcttcatatc ctatattcat agcagtaaca
     8341 aaaaaatctg gtaaacattc ttcatctgta atacctttct tgtccatata ctctttaaca
     8401 attggagtat atactttata atacacatcc tcatcatgca agctttcagt ttttcttata
     8461 tatgaaagat taaatatagg ttctattcca ccactaatac ctatcattgt agatatagaa
     8521 cctgtaggag gtatggttaa aagttgtgaa tttctaagcc catacttttt aaccatttcc
     8581 ttagtgtctt catttagatt ttctataaaa aacttagatt tagaaatatt ttcatattta
     8641 tactcactaa atgttccata ttctttagca attagtgctg attgcttcac agcttcattt
     8701 aacatagtct taccaattaa atcacatagt tctaaagatt catcagaacc atatcttatt
     8761 cccattttta taagcatatc agctataccc attacaccta aacctatctg tctatactta
     8821 tgtacactat ctctttgttc ttgtaatgga tgcaagttca atccctcatc taagacatca
     8881 tttaaagcta ctacagcttc tctaacgcaa gattttaatt tattaatatc aaaagtagca
     8941 tgttcaccaa atggttgtat tataaactct gacaagttta ttgagccaag tagacaactt
     9001 cctccacttg gaagtggttc ttctgcacaa ggatttactc ctgaaaagca aaagttctta
     9061 tcttcactca taaaattata tgtttttatt ttatcccaaa acaatatacc tggttctgca
     9121 taatcccagt tatttttcac aaatttatta aataaatcat aagcatttac tatcttaact
     9181 attttttctc cagtagtttc aacttcaaaa gtacaattat ataacttttt ttctcttaca
     9241 gcttgcataa attcatctgt tactcttaca gaaatatttg ctttagtaac cttctccaaa
     9301 tctgttttta tatctataaa gtcttctatc tctggatgat ttacatccat agaaatcata
     9361 agagctcctc ttctccctct ttgaccaatt aagccagttg tcatactgta aagttccata
     9421 aaagagactg ctccagtcgt atgttttgca gcattgttaa ctttcgctcc ttttggtctt
     9481 agctttgaaa tatcaattcc aactcctcca ccataactaa aagttctagc aagttttttt
     9541 gcagtatcaa atatatcttc tatgctatct tttggtggtt ctattacata acaattagaa
     9601 tatgttactt tttttccttc tttctgtaat cctctatttg ctaaaattct tccagcaaat
     9661 agaaatcttt tttgtcttat tagttttgat attcttgaac tattatttga aattctcttt
     9721 atccattcat caaaactctc accattatgt ctatacttgt tcttccatat tgatttttgt
     9781 aattcatcaa actcccaagc tttagtcctt atttcagagc gtctttctct atataatatg
     9841 tagtgttttg ctatatcttt tctatttgat tccataagta gtacttcaac tatatcttgt
     9901 atttcttcta cggataagca tttttccata gaattatagt cattagctat ttttcttgat
     9961 atatcatatg ccaactctct atctactcca ccttcactat ttatactagc tttatagaca
    10021 gcatcttcaa tcttactttt actaaaaagt tcttttgtac catctctttt tattatgtat
    10081 ttcattttta aacctccaat aaattagtat taaattaata cacacattaa ctataactct
    10141 aaacaaactt tttatatatt tgtatatttt catattaatt atctaacata atatatatta
    10201 tact
//
LOCUS       DAMJWS010000116        10191 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMJWS010000116 DAMJWS010000000
VERSION     DAMJWS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.121"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(141..2021)
                     /locus_tag="PC399_003364"
     CDS             complement(141..2021)
                     /locus_tag="PC399_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF2594192.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2225..8188)
                     /locus_tag="PC399_003365"
     CDS             complement(2225..8188)
                     /locus_tag="PC399_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HDF2594193.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8312..8397)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8569..9972)
                     /locus_tag="PC399_003366"
     CDS             complement(8569..9972)
                     /locus_tag="PC399_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF2594194.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         2803 a         1886 c         1157 g         4345 t
ORIGIN      
        1 taaacttaat atagctcaaa ttattataca ttttaacctc aatattatta gattgttaag
       61 ataatgaaaa taaaataaga atgcatttaa atatataact ttatatctaa atacattctt
      121 attttctatt tacttaatat ttatttttca attaaactct taatcttgtc tattacagat
      181 gaggctattc ctccacctac ttcaaacaac tttgatgaat ttttaccttt taatgacgat
      241 tcttgtgatt tatctaacgt atttgtagca agtataactg gtgaattgtt tttagctgct
      301 aatggtccaa ctgctaaagc atctataagt ttatcttgat ttgcaactac aactccatct
      361 agcttagatt gagagtagaa tttatctatt actttggcat tcgtctcttg tctagtttga
      421 cctgccaccc tattgtttga tatatctttg tttgctattt ttccaacttg ttcaatagcc
      481 ttgttagata ttttttctac tccaccaata aaatatatat ttgacacctt atttgattta
      541 ataaattgtt cagcttcatt atctacacca tctttttgtg taagtactat tggagttttt
      601 tctttaccag ccagtgatgc tattgataat gaatcagctt ctccatttcc tgctcctata
      661 tacaacttgc ttacattatt cgtcttatct atttcttttg ctatattaat agaagtttga
      721 tatcttgtat ctccacctat tctttgtact gatatttttg aatttacagc tttgatagct
      781 tcaacaactg aatttggcat tgagttgttt ccacctatca caattacttt tgatggattt
      841 agccttttca attcttctac agttttttga ggaagctttt catttgatga taaaagaatt
      901 ggtgagtttt ttacactagc aagtggtgta gctgtaagcc catccaccat acttttatct
      961 tctccattta ctattacaac tgtatctgat tttgtccaac cactttgaga tattttgata
     1021 gctgtctcaa atctatcagc accttttaat tttttgactg tgacattaga tgtagaatca
     1081 cttgaacttc ctcctccaga gcttccacca ctagatgagc cgcctgaatt acttcctcca
     1141 ccattatttc caccattgcc attatctggt ggtaattcat ccggttttga taattcattt
     1201 tcaactatgt attctaaact ttttggaagt gtaacttcaa catatttgtt tattgtagct
     1261 tttcttccct tagttgtttt ttgtgctaat ccaagtttta atattacatt tgaaccactt
     1321 tcttgtgttt ctttactaac aatagaatag tttatcctaa tattgtttaa taaatattca
     1381 agtgcttcac ttttctctac cttatacttt tcagggtcat ttttactaga accagttcca
     1441 ccaattggtt ttacttcact ctcattaata agggtaaata ttaatccttc atcagagctt
     1501 atattttcat aatccactgt tttgttgtta tatgttacaa tttttaaagc tggtttatca
     1561 tttgttcctt ttcctaaaac tttatagtca gcatttgagt tactgccaga tttttttaca
     1621 gatattcttc ctatattcaa tctatttccg tctagtaaat cttcatttga tgtaacatat
     1681 atatctaatg tagttgtatt acttgatttg ttgtattcta ctctacaagt agcatttgca
     1741 tttccacttt ttatctgaga ttttatatta tcagaccaag taatcttttc tttctgctca
     1801 ttatctacta cttcaacctc accttgtact tttatactgg cttgaaatga atatactatg
     1861 ttttctactg agtccacaga tatatctata gtatttactt tctcatacgt ttttgttcct
     1921 tgtaaaactg gagctgcata tgcacatgta gcactaccag ttacagctac tgccatagtt
     1981 gatactgcta tagttcttat aaaagatttt cttttactca ttaacacccg tccttatcta
     2041 tcattaacta agtggataaa tttacttatc cacctagctt ttatatttaa tttataaatt
     2101 accctaaatt tatggttaca tttatatact ttacttttaa tatctactat tttaaaacta
     2161 ttctagtacc taatcttatt agatatacaa tgttagtatt tttacgtttt tagagttcca
     2221 cctactattt atctctaaca atattaccct ttactttatt gcttacagtt ataaccttct
     2281 tattactgat atcaatagaa tttcttgtag cttttctagc tttactatta ctatcagtaa
     2341 gacttattgt ctttaactca tcataattct tagctcctac aggtacatac attgtatcac
     2401 ttacaaatct ttgacctact ggagtatctc cttgaaggtc attgtatcta tatagttcag
     2461 ccttaacctt atcacttaaa gaatcaaagt tttcttttgg catatagtaa acccatgtac
     2521 ctttactgat ttcacctagt tcaccttttt ctggaacttc tgcaaataaa agtgcttctc
     2581 cagatatagt cttattgttt ttatctataa gtagtggtaa attaaatgct ggatttccac
     2641 tgtattcacc tgtaataact attgaacccg ctggaatctt acctgtttca tcttcacttg
     2701 tgctagagtc atcactttga tatacataat cttctgctaa ttttccaatt ccattagtac
     2761 gagtttcgtc tgcattattt attatgccaa tttctatatt atcacctgga ggtcctatta
     2821 tatctaattc tgcaacttct atattagact tattatttga gattagtttt acatatctag
     2881 cttggtatgt ccaaagttta ccatctttat taaatgtaac tctagcttca ttatcttggt
     2941 cttttcctcc accaactaaa tcttgtccat attcaaaagt tccttgactt acatcttccc
     3001 agttcttttt atcattactt acttgtatct tgtaattagt taaaggattg tatgttgtgc
     3061 tagtattctt tctaaagtta aataatcctt ttaatgagaa ctttctagct tctgtctctg
     3121 gttttgtata ttttagtcct actataggta atgtttcttt catgtctact attatatatg
     3181 gattttcttc tacatttata tcagggtctt tttgagggtc tttgttccac tctgctttac
     3241 ttattatctt tcctttatat gtagtacttg catcattatc ttttatatta tcaatttcag
     3301 gattttgtaa tggtccatgt gagtcatttt cagttctaac atcaccgtca gaaacagtat
     3361 ttgttatgaa tgaccatgct gatttattta ttttcccttc atctgataat tttacagtac
     3421 caacttcaaa ttcttctgtt ggatttaggt gataatcata tgcaactacc ttatatgtaa
     3481 atgctctgtt attaagagga gatatattat cagtaaattt agtcttgcta tttccttttg
     3541 catccacaaa tccaactgtt ctgtatttta cctgtgttcc attttcacca tctaaataat
     3601 ttccatcact tcttattatt tcgtatccta gtatcttatc tttatcctta tctacatcta
     3661 ggtctataga tattttatta gatttaacta gagaaccact ttgtattcca tcagaaaaac
     3721 tagcacttac tttagtatct tttgccatac tagctatatt ttcatttaaa atctttcttc
     3781 tagcttcatc atttacatat tgtatctttc ttgattcttt ttcgtagttc ttgctatcca
     3841 ttatactgtt taattctggt gtaatttcta ctccccatgc cttaaagtaa tctcttaaat
     3901 ctttttcaac aacacttgat gccattgcta ctagcatttg gtcttttggt aaactattaa
     3961 ttgggtcact acttgaaagt gctctgtatt ttcttgaaat ctttgcatag aatgtatcat
     4021 tacttaaatc agcatctgag ttattcttta acattgaacc cgtaaaatca tcatcataag
     4081 ctagatgtaa ttgccataac attccaagcc tttcaaatac attgcttggt atacctaagc
     4141 tatttgaagt tacatgctca tatatcttat ccattgttga accttcaagt cttgaatgtg
     4201 tcttatcatc aaaggtctga gcaagtaaag caagtacatt gttactagtt tcgctatatg
     4261 tcatctttcc aatatctgta acatgtccta tctcatgacc tattccccat ccaaataagt
     4321 ttcctcctgt agcttttcca ctatcatcta tttggaaagg tactccattc ataagccctg
     4381 gaactgaacc atattctact cctacatgta atccagaagc atacataaat gctccaataa
     4441 acattctttg gtattttatg ttcatacgag attttggagc catatcattt ttagttaagt
     4501 taaattcatt atcatctaca gttccattac catctaaatc ttgtgtttta aatactcctc
     4561 tttctgagta aactaaatcc attatttgtt cccatgcaag catactatta tataatctat
     4621 ctacttgtgt atttaaatca gaatttcctt ttgatatctc ttgatatact gctgttgcag
     4681 gaacatttaa tgttacttta tcactttcaa tatctgtagt gtttaacaca ccagtttttt
     4741 ctgaatatcc atatgaatta ccaacatttg ttcctggata atatggttct ccttttacag
     4801 catctttata tttagctaat tcttctatat atgcttttat ctttttctta gattcagaaa
     4861 ctcccgcagg actcttatca tttattgttc catttagatt aagatgtggt atcttagtag
     4921 ctcctgctac acgtactttt atatctcctc cacttggagt atcttcagcg tatcttatgt
     4981 ataaacttcc tccacattct atatctaaac tatgaagttt gcttaattga attacatttt
     5041 ttccagtgtt aagatttatt tctccactct tatatgcacc agattctcca tagtactgtg
     5101 tatatatcaa cttaggagtt tttccacttt tacttccaac atatactgtg atttcatctc
     5161 ctgctctagc agctactcct aatgcctgcc agtcattttg catacctaaa ttattggaat
     5221 ttacagtata ctgtccgttt ttatctttat attttcctaa tttatttatt tcttgattta
     5281 cagtagttac atcagtaagt gtggctctat cattatataa gtcttgagct aattttaatt
     5341 ctctctcaat aactgtctta tatggatgat attctccact agctttatct ggagtattta
     5401 atctatcttt taattcagtt attttttctt gagttacatc ttcttttaat tcaacttgta
     5461 ggccatccat aaatagattt cttacatcat cttctatact gtcatactca taaaacttaa
     5521 gttctgatat attagactta tatgaatagg atgaacaaac acttacttgt atcttggtag
     5581 cagttattgg ttcttttaat ctaaccatta catacttatt attatttaca gggtctgtct
     5641 ttgtttcaaa aggaacatcc cccagattgt gtaattgacc attttcatcc caatatcgta
     5701 cctcaaagtc atttattgtc ttatcagaaa catcaaaacg aggtgtagcc ataaaagtat
     5761 ccatagtata agatttatca aaagttatta ttggtcctct attcgttccc tcagctgcat
     5821 tatatgttgg gaatgtccaa tgtgttgtaa agtccccatc tactacattg aatttatcaa
     5881 atggattttt atcactatat tcacttttgt catatccatc tggataaaca acatctacaa
     5941 tgtgatttgt tggtattcca aatccaccat tttctttagg agtattaatt aatctatagt
     6001 taggtgttgt tggtgcatcc atgctttcag ttgtacctat atatgtttta gacattccac
     6061 tagttcccaa gtggttagta gctgtcattc tgacttcata tgttgtatta tccttaagac
     6121 cttctatctt tgaacttgtc ccacgaataa gcttatcatt gtattttatc caagacccat
     6181 tagagccttt ttctttatag tacaagtcaa aatctttagc ttgtttatga tttttccagc
     6241 ttacatcaag agatttaaat ttacctttaa catttatacc ttctggtggt tctggttttg
     6301 tgtctggaac aacttgaaat tctaatatac tatctgggct aaattgtata ggtttatagt
     6361 ttgaatctac attttctggt actccgtcta agttttctgg tgttgcatct ttataaccac
     6421 tagaccaatc tccatttaaa gattgtatgc taactctata tagtccatat gcttctatat
     6481 ctctaacttc tattgtattt ttactagttt gtaatttttt agtagataat acttttccct
     6541 tactatcaat tttgtctagt ataagttcgt atgctgatac atttacttca ttattccaat
     6601 aaaaagatac tttttcattt ccaacagcag tgctagtcga tattttattt attttaggta
     6661 tgttcatctc tggctttggt atttctttat atacattgtt taaaaattca acttttgata
     6721 tttctgcaag atttctatcg tttgctgttg tagctgttat ttttatagtt atctttttta
     6781 ctgctacttg tttaccaaga tttattacta tatcactatt ttgaacatct gcatatgttt
     6841 ctaatgctga cctactatat gtagatgttg tagagttatc atctttactg atattaccct
     6901 cttcttttgg agtatcttca tctgaattag gtttatccat tgatgaattt tctttatcat
     6961 tatttttatc ttgattggta ttagagtttg cttcttgttt atcgctattt tcctgttgtt
     7021 tatcattatt ttcatcttta ttatttgagt tattatcagt ctggtttata ttttcatctt
     7081 tattatttac ttcttcttga tttacttctt cttgttcgtt atttacttcc tcttgttgct
     7141 cctcatcttt taagtttttc atatattctt tgttttcata aggaatctgt tgaacattac
     7201 catctttgtc agtaacctca actattcctt ttgctggagc agtaccttct ggagctttta
     7261 tattgatgac ttccatatta agtgcttctg ataaagctat atccattact gctggttttt
     7321 cctcagatat ttcatcattt tttgaagata ttgtaactcc attattttct ccactaaata
     7381 aattttctac attaccatta atatctacat cactatttcc ttttaattca accttacttg
     7441 tatctactat aggattggta gatactatac tttctaggct ttttgcatct tcactttgtc
     7501 ctaaattttt ctgtacataa tctaagtcaa caatatctac ctttccatct ctatttaaat
     7561 caaattctct tttatttgaa tctatattct ctaaaacctt attataatca ctcttatcta
     7621 ctttaccgtc tccatttaca tcacccatta agaacaattc ataatttaaa ggtttatttt
     7681 tattatctac acttgctcta ttgcttaata agactctttg tgaataactt gatatatcaa
     7741 tgtctttaat ttctttgttc ttaaatccat ctccagagac ttctacattg tattttccaa
     7801 caggtaaatt attaaataca atattataat atgatatatt ctcatcttta ttttttattt
     7861 cacttctact tgcagataat ttttttatag tatattctat attatctgtc ccaacattta
     7921 ctgttcctga atctttttct ccaagcttta ttattccact attgttacta tcatctttta
     7981 atcgtaactg catattagtc atactttctg actcgctatt ttttattggc agtgaaaaat
     8041 ttaaatctac ttcaagactc ccccttaact tttctgtaac tgatttttca actgccctta
     8101 gcttcccctc taatataggt tctgcaaaaa cgtttagtgg tgcactacac atagatactg
     8161 ctagtacaca agatattgcc tttttcatga aataacaatc ctcccttaat aaatatctaa
     8221 tttttcatga atttaaatac tataagtttc agacaataaa aaaagacact caaagagaat
     8281 cttaattata aaataaaaag actttactct ttttagcaac tggctgatat ggctttaaaa
     8341 gctttaatcc ctatagtttt gcgtcattag atttccctaa ttttgccatt taattttcac
     8401 tactttctta cccattttat actatattta aactttttaa tataattttt ttagattaaa
     8461 ttataaaaaa ataacattta tattaattta ttaatataaa tgctattttc cattataatt
     8521 taattattta tatagcttcc ttacaagcat aattttgtgt atattttctt attctttata
     8581 tcttccataa aattcatgtg tagcatatag attcttatct cctgttatag ctataccaaa
     8641 tcccattatt tcataccctt tatgcaacat attagttcct tcttctggat ttgtcttcca
     8701 ctcattaaaa ataaagtttc ctatatcatc tgcatctcta tttgtatata caggtttatc
     8761 tggaatttta tatttaatta tattttcacc ttgaactgct atatatccag gtttaatctc
     8821 actccaatct aagtatttca aaaaagtaga atatgaattt tttccattta tcgtatgaga
     8881 taaagtcttc ttatttgcca ttaaattaga ccaatcattt gctagaccct ctaatctgct
     8941 tgctacagat aacgctctta ctccattctc ttttctatac tgctttgtaa gagcatatac
     9001 tttacccttt attctctctt taaatgctct atcatttgga ttgtaatata aaccttgttg
     9061 atttggtgaa gcttgattta tacattgctt gataacattc tgatttatgc ctcctagtac
     9121 agtcaatttg tctgcaccac ttaaaatact cttgttactg ccattttcaa ctaaaactac
     9181 aggagcattt tttgctattg tagaggcagc caaagcgtct gttaattgta agcctttact
     9241 tagataaaat tctttactat ttttataaaa ctcttgtata actatcttat tagtttcaaa
     9301 tctatctgtg ccacccaatc taatagaatt tgtgctctta actaattcgt cgcttattat
     9361 ttcactagaa ccaagcgcat aactctttaa gcctgtcgtg tcaaatccca cactatttcc
     9421 atctgtaagt attataggta ctccatctct agcagctact ggtgatgcac ttatcgcatc
     9481 tgcttctcct gtaaatccat tggctataat tactttatct attggcttaa tttctttgat
     9541 tttttcagct atttttatac ttgtatcaaa tctattttca ccaaatattc tttgtacaac
     9601 cttctttttg gatagtaact gattctcaac atttttactt attgttgatt ctgaacctat
     9661 tatatacaca gtctttgctt tgtttaatct ttccatagtt gtgtttggaa tctggttttg
     9721 ttttgtcatc aaaataggcg catttaaggc tccagctaaa ccacttgcac ttagaccatc
     9781 tgctaagcta ttatctgagt tgattagtat tgctgtattg tagttttgct tatctgctat
     9841 ttttgctgct gtctcatatc tgtcattccc ttttatttgc tcaactgatg tcaacgcatt
     9901 taccatagga gtatttacca ttactaaaaa tagtgtaagt cctatagcta atatcttttt
     9961 actattcttc atattatttt ctacccccaa tgtacttttc atatttttta ttagtactca
    10021 tacttatttt taatcaaaag ttacaatttt gttaccatat tttatatatg ataccatagt
    10081 tatccataca actcaaacaa tatacctaaa gtttttattc tctataaatt caaaacatta
    10141 taatttattt tatttaatta cgttataagt tcaaaataat ttatgttagt a
//
LOCUS       DAMJWS010000117         9677 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMJWS010000117 DAMJWS010000000
VERSION     DAMJWS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.122"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            171..1457
                     /locus_tag="PC399_003367"
     CDS             171..1457
                     /locus_tag="PC399_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2594195.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1569..2042
                     /locus_tag="PC399_003368"
     CDS             1569..2042
                     /locus_tag="PC399_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1701..1703,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF2594196.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2074..3384
                     /gene="grdB"
                     /locus_tag="PC399_003369"
     CDS             2074..3384
                     /gene="grdB"
                     /locus_tag="PC399_003369"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3121..3123,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2594197.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3640..5172
                     /locus_tag="PC399_003370"
     CDS             3640..5172
                     /locus_tag="PC399_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF2594198.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5183..6328
                     /locus_tag="PC399_003371"
     CDS             5183..6328
                     /locus_tag="PC399_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF2594199.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6638..7717
                     /locus_tag="PC399_003372"
     CDS             6638..7717
                     /locus_tag="PC399_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2594200.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7830..8429
                     /locus_tag="PC399_003373"
     CDS             7830..8429
                     /locus_tag="PC399_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594201.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8616..9488)
                     /locus_tag="PC399_003374"
     CDS             complement(8616..9488)
                     /locus_tag="PC399_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2594202.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
BASE COUNT         3716 a         1115 c         1849 g         2997 t
ORIGIN      
        1 ttaaataaaa taattttttt acaataaata ttttttagta ataaaataaa gatttaaggc
       61 ataaaaacac aataatatat ctttaaaaaa ataagataaa ttgtaaaatg atataatttt
      121 gagaaaaact caaatgcata tacacaaatt aaataacagg aggtgcaagt atgcgtcttg
      181 aattagggaa aatctttata aaagatattc aatttggtga tgtaacagaa gtaaaagatg
      241 gagtattatt catcaataag caagagatgc tacaagaaat tggtggagat gagcatatta
      301 aatctataga tatagaatta gctcgtccag gtgaaagcgt aagaataaca cctgtaaaag
      361 atgttattga gccaagagta aaagttgaag gtaatggggg aatcttccct ggtatcatgt
      421 ctaaagttga cactgtagga gaaggaaaaa ctcatgcttt aaaaggtgta gctgttgtaa
      481 ctactggaaa aatagtaggt ttccaagaag gtataataga catgactgga gaaggagcta
      541 agtacactcc attttctaaa ttaaataatg tagtagtaat agctgaacct attgatggtt
      601 taaaacaata cgctcatgaa aaagctgtta gaatgatagg attcaaagca gcaatgtact
      661 taggagaagt agcaagaaat cttactccag atgaagtttc agtatatgaa actaaacctc
      721 ttcttgaatc aataaaagaa tatccagagc ttccaaaagt aggatatgtt tacatgcttc
      781 agactcaagg tttattacat gatacttatg tatatggagt agatgcaaaa caaatagtac
      841 caactttatt atatccaaca gaattaatgg acggagcaat tgtaagtggt aactgtgttt
      901 ctgcatgtga caaaaaccca agttatgttc atataaacaa tggggttgtt gaagacttat
      961 atgcaagaca tggtaaagat ataaactttg taggtgtaat tataactaat gaaaatgttt
     1021 atctagcaga taaagaaaga tcttcaaact ggacttctaa attatgtaaa tatttaggat
     1081 tagatgcagt tatagtttca caagaaggtt ttggaaatcc tgatactgac cttataatga
     1141 actgtaagaa gatagaaatg caaggtgtta aaacagttat agttacagat gaatatgctg
     1201 gacgtgatgg aggttctcaa tcacttgcag atgcagatgt aagagctgat gctgttgtaa
     1261 caggtggaaa tgccaaccaa gttgtagttc ttcctaagtt agataaagtt attggacatt
     1321 tagaagtagt agatgtaata gctggtggtt ctgatggaag tttaagagct gatggaacta
     1381 ttgaagttga aatccaagct ataacaggtg caactaatga aacaggattt ggtcatttaa
     1441 ctgctaaagg atattaagaa gtattttatt aaacgattga gtaatgttaa taaaacatat
     1501 tatagataat tataataagt gttatagata tataaaaata tattagaaat ataataagga
     1561 ggattattat gagtttactt agtaataaaa aggttcttat aataggtgac cgtgatggta
     1621 taccaggacc tgcgatagaa gaatgtgtaa aaacagtaga aggagcagag gttgttttct
     1681 catctacaga atgctttgtc tgaacagctg ctggggctat ggacttagaa aatcaaaaca
     1741 gagttaaaga tgctgctgat aaattcggag ctgaaaatgt tgtgatttta ctaggtgctg
     1801 ctgaagccga agctgcaggt cttgcagccg aaacagtaac tgctggagat ccaactttcg
     1861 ctggaccact tgctggagtt gccttaggat taagtgttta ccacgttgtt gaggaaccaa
     1921 taaaatcatt atttgatgaa agtgtatatg aagaccaaat aagtatgatg gaaatggttt
     1981 tagaagttga agaaatagaa gaagaaatgt ctggtataag agaagaattt tgtaaatttt
     2041 aatgcctaaa agcaaaaggg gtgaaactaa gagatgggaa aactaaaagc agtacattat
     2101 attaatcagt tctttgctgg tataggtgga gaagaaaaag ctgatactaa gccacatgtg
     2161 gcagaaactt tacctccaat aagtttacaa cttgataaat tattaggtga agatattgaa
     2221 atagttggaa cagtagtttg tggagatagc tatttcaatg aaaatataga tagtgcaagt
     2281 gaagaagttt tatcaatggt aaaaggtttt gaaccacaac ttttcatagc tggtcctgca
     2341 tttaatgctg gtagatatgg ggttgctgct ggaacaataa ctaaagttgt aaaagatgct
     2401 ttgaatatac cagctttaac aggtatgtat gttgaaaatc caggagcaga tatgttcaaa
     2461 aaggatgttt atgtagtaga aacttctgat tctgcagcag gtatgagaaa ggcacttcct
     2521 aagatagcta aacttgcagt aaaacttgct aatggagaag aaattggaac tcctaaagat
     2581 gaaggataca tagctagagg aataagagtt aactacttcc atgaagacag aggttctaag
     2641 agagctgttg acatgttagt taagaaaata aaaggtgaac catttgaaac tgaataccca
     2701 atgccaaact ttgatagagt tgacccaagt aaagctgtaa aagatttatc aaaatgtaaa
     2761 atagctttag ttacttctgg tggtatagtt ccaaaaggaa atccagatag aatagagtct
     2821 tcttcagctt ctaaatatgg aacttattct atagcaggag ttatggattt aacagaagag
     2881 acttatgaaa ctgcacatgg tggatatgac ccagtgtatg caaatttaga tgcagataga
     2941 gtattacctg ttgatgttct tagagattta gaaaaagaag gtgtaattgg aaaattacat
     3001 gagactttct atacaactgt aggtaatggt acatctgttg caaactctaa aaaatatgct
     3061 tctgaaattg gagcagcatt agtggctgat ggtgtagatg cagtaatatt aacttctaca
     3121 tgaggtactt gtactcgttg cggtgcaacg atggtaaaag aaatagaaaa aactggactt
     3181 ccagtagttc atatgtgtac agtagtacct atttcattaa cagtaggggc taatagaata
     3241 gttccaacta tagcaatacc tcatccactt ggaaatccag cgcttgaccc tacagaagaa
     3301 aaagccctaa gaagaggcct tgtagaaaaa gcattaaatg ctttaactac agaagtagat
     3361 ggtcaaacag tatttgaaaa ataaaaataa cacacacata tattatatag ataaaaatat
     3421 aaacacacac taaaaataaa aaataactat acacaaaaaa ataatataga tttcatgaat
     3481 ggataaattg gagttattca ttcatgaaat cttaaaaaat gccaaaatac ataaacaata
     3541 atagaatcaa atatacaata aaatacaatg attagagctt ttaataagtt taataagatt
     3601 aaaaaataag tgttcataaa atttatggag gtgcacaaaa tgacttatcc agttttaaaa
     3661 ggggcaggct atgtacttat tcatactcca gatatgatag tgcaaaatgg aagtacttgt
     3721 acagttgaaa gagcaacaaa tccagattca gaatttttaa aagaagtaag taatcatata
     3781 agaagttatg aagatgtagt taactacatg ccaaatcaag tttatatagg aaatagaaga
     3841 ccagaagaat taagagattt accaatgcca tggtgtgaac aaaagattga gggaactaga
     3901 aatggtaagt ttggagaaat aatgcctcaa gatgagttca tagctttaat gcaaataagt
     3961 gatgcatttg atttagtaaa attatcacaa gaatttattg atgaagtaaa gccaaaaatc
     4021 gaaaataact accctgaaat tgcaccattt gtaggtaaat taaagggtga tgatatagaa
     4081 gaaggtaaag aattagtagc aacacacata gctgaaggat tataccatga tggaaaattt
     4141 gttggttatg taaaaagagc acatgatgta gatgttaact taaatgccca tacaatgttt
     4201 gaaaatctag tagtaaaagc atctggagtt ttatctgcta ttcaaatgct aagacatagc
     4261 aaaatagacc ctgctgaaat agattatgtt attgagtgtt cagaagaagc ttgtggagat
     4321 ataaaccaaa gaggtggagg aaactttgct aaatcaatag ctgagatagc aggacttcaa
     4381 aatgcaactg gttctgacac aagaggattt tgtgcagcac caactcatgc attaattcaa
     4441 gcagcagctt tagtaaaagc tggcatacat aaaaatgtaa tggtagtagc aggtggagca
     4501 agtgctaaac ttggaatgaa tgctaaagac catgttaaaa aaggtcttcc tgtacttgaa
     4561 gatgttgttg gaggatttgc agtacttgta tctgaaaatg atggtgtaaa tccagttata
     4621 agaactgatt taacaggtaa gcatactgtt ggaactggtt cttcaccaca agcagttatg
     4681 acagcactta taacttcagg tcttgataga gctaatttaa aaataacaga tgttgatgtg
     4741 tattcagttg aaatgcaaaa cccagatata actaagccag caggagcagg agatgttcca
     4801 gaagcaaact acaaaatgat aggggcactt gctgttaaac gtggagactt agaaaagaaa
     4861 gaattgaaag attttgtttc taacaaagga ttaccaggat gggcaccaac tcaaggacat
     4921 ataccatcag gagctcctta tataggattt ttaatagatg atttaactac aggaaataga
     4981 aatagagcta tgatagttgg aaaaggtagt ttattcttag gaagaatgac taacttattt
     5041 gatggagtat cttttatagc tgaaagaaat acaggtgtta ctgaagagac ttcaggaata
     5101 tctaaggatg aaattaagaa gataatagct gaatctatga aaaaactagc attagatatg
     5161 ttagaagaat aggggtgagg atatgtcaaa aaaagtaatc gcagatgtat tcttagaagt
     5221 agctaatgca atagaaagcg gcgaatttgg caaaaaagta aaaattggtg ttactacact
     5281 tggaagtgaa catggtgtag aaaacatggt taatggtgca caattagcaa aatctaattt
     5341 atttgatatt gtgcttattg gaccaaaagt ggaaactgac cttgaagtag ttgaagttaa
     5401 tgatgaaaaa gaaatgcatg caaaaatgga agaattatta gattcaggat atattgatgc
     5461 ttgtgttact atgcattata attttccaat aggtgtatct acagtaggta gagtaataac
     5521 tccagcaaaa ggaaaagaaa tgatacttgc aacaacaaca ggaacaagtg ctactaatag
     5581 aatagaggca atggtaagaa atgccattta tggtattgct acagctaaat caatgggtaa
     5641 caaatgccca aaagttggaa tacttaatgt tgatggagca agacaagttg agaagtgctt
     5701 aaaagaatta aaagataatg gatatgacat ggagtttgcg gattcaatta gagcagatgg
     5761 tggatgtgta atgagaggta atgatttact tgtaggtgca cctgatgtta tggttactga
     5821 tacattatct ggaaatatat ttatgaaagt attttcttca tatactacag gtggagatta
     5881 tgaagctcaa ggctttggat acggacctgg agttggagag gactatgata gaaaggtact
     5941 tatagtatca agagcatcag gttctccggt agtagcaaat gctttaaaat acgcttatga
     6001 tgttgtaaag ggagatataa gtaatgttgc aagaaatgaa tttgcaaagg ttaaaaaagc
     6061 aaaatttgat gacattatat cctctcttac taaaaaagaa gttaaagctg aaaaagtaga
     6121 agttaaaatg ccagataaag aaattgttac tcgtcaaata gctggtgttg atataatgga
     6181 tttagaagat gcagtatctg agttatggaa aaacggaatc tatgctgaaa gtggtatggg
     6241 ttgtacaggt ccaatagttt tagtaaatga agctaaagga gacttagcag ttgaaacttt
     6301 agttaaagct ggatatactg caaaataagt acatttttta tatgtaaata agaacatata
     6361 gttataattt tattgattat aattatatgt tctttttatt ttatatcttg tcaatattta
     6421 tatattaaca caatccattt atttgattct ctagctaatt tattatattt attcaattaa
     6481 aattaagctt tctaactaaa taaaaactaa tgaagtatta aaatgaaatt gataaaatat
     6541 gaaaaaattt taggaataaa gtatctgtta gataaaaata agattaatta gtgatataat
     6601 ataattaaga taaaatttat tatttggagg taaaatcatg agcagagtaa aaaatgtagt
     6661 agaattgttg gaaactaaag gtgttgatgc actatatcta acaaagaaaa ctaatgtaaa
     6721 ctatataagt ggttttcctg atgaagaagc atatgcagtg atttgcaaag atggaaactt
     6781 tttagttact gatagtagat atatggagct tgcagaaaag gtttgcaaag attttgaaat
     6841 tataaattgg cataattttg atagaagtgt ggcaaaggca gttaaaagtg tatgtgataa
     6901 agtgggtata aagaaacttg gatttgaaag aactaatata gtgtttgata aatatgaaga
     6961 acttaaaaat ctgattgaga aggataatgg agaattaata cctacagaaa atattgtaga
     7021 gactttgaga tatgtaaaag acaaagatga aatcaaaaat acaagaaaag cttgtgaaat
     7081 agcggataag gcattagaag aattgattcc acatataaaa gctggagtta gcgaaattga
     7141 actggctaca aggttagagt actttatgaa aatgaacgga gcacaaaata tagggtttga
     7201 aactatactg atatcaggag caaagacttc attgcttcat ggtaagccta gtgataagat
     7261 tatagaaaaa ggtgattttg tacttataga ctatggtgct atgtataatg gctatatatc
     7321 tgatacaact agaactttta tagttggagg agcttctgaa aagcaattag aaatatataa
     7381 tttagttaag gaagctcaaa atgtaggggt tgagaacatg aaagctggtg tacatgcgac
     7441 aattccagat gctgagataa gaaaagttgt caaaaaatat gaagactatt actatcaagg
     7501 tataggacat ggagtcggta gagatgttca tgaagaacct ttcattggta attatggtga
     7561 taaaatcata gaagagggat gtataataac aatggaacca ggtatttatt tccctggatg
     7621 gggtggagtt agaatagaag atactgtttt gattactaaa aatggtccag aaagacttac
     7681 taaattccct aaagatttga tgattttaga caaataattt aatttatata tattttttta
     7741 cataaaatat aacataatct cactattaag ctctatttac atattatagt aaagagggct
     7801 taattttatt gtgagaggtg atagtatata tgtataaaaa acataaattt ataaaaaata
     7861 taaaaaaagc ctggtataaa atcaaattaa ttgatagatg ccttataatt gtaatggcaa
     7921 tattaatgtt tcaatctata tataacttat ttgttaatga agtaaattca caagatacta
     7981 caactattga tgtagtagta agaaccactt cagcagctat atttgggtat tttttaagtg
     8041 ctaattttat aaagagacct ttaaggaagt atacaaatac tgatgtatct tcaaatcttt
     8101 ttatagataa taatttatcc ccaaaaggtc aagagtctaa tcaaaataat ataatgaatg
     8161 tcaaaaatac cattggattt acttcagaag ctgataatta tgaaaagaaa atacctatta
     8221 ataataatga aggttttgaa gaaggagaaa ctagtgaatt gcaaattatt atagctactg
     8281 taatttgcat agtagcatta acagtacttt ttgttgtaag aaattttaca actacaacat
     8341 cagcttcttt aggtactatt tcacagatga gagattttgt ctcaggatgc gtgggttttt
     8401 tgctaggttg tccaagtaaa cctgattaaa aaagtattat caataaaagg tattttaaaa
     8461 agtatcagta taataatatt tagatttaaa tgtatacaat tataattctg taacattcta
     8521 tattgtattt ttatactgat actctaaact atactattta ttaaaatatt ttaacattgt
     8581 atttaaacaa aatattatat ctgtataata taaatttagt ttatatgaat agatgccata
     8641 tactcaataa aaagtttcat actatgagat atgtattgat ttcttctcca aagaacccca
     8701 atatttgcag taattgcacg ttctaatggt attgaacaga gattttctgt attttgaaat
     8761 atttctttat acaatattgt tgaagccaag tcattttcta gtaaataacg tatggtagaa
     8821 agttgatttg agtgtaaaat tatatttggc ttagcttttt caagtctttt tgtaataatc
     8881 ttatagtgat aagaaccttc agataagatt acaaaaggta cattttttaa catataattc
     8941 gatataatag gatgttttgc caattcattt ttgggatttg taactaagta taattctgtt
     9001 ttatagattg tctttgaatc tatatcatca taaatatcac tgtcaattat accaatacaa
     9061 aaatcaagtg tagattcttc aatcatttta gctccaacaa atgttggaac ttcaaaaatt
     9121 ttaagctcta tatctggata aattacatta aacctaggaa gtatttttga aagcaaaaac
     9181 gtacctaaaa ctgtaggaat acctattttg agagaagctt ttctcttttt tcctaaatca
     9241 agtgcgtcac attgaaaatc ctcaaattgt tttataaaat gttttgactt gtctaaaaaa
     9301 atttttcctt caggcgttaa ctctatttta ttacctattc tgtaaaaaag ttcaaagcca
     9361 agttcttttt ctaattcttt aattgctaat gttatggatg gttgagaaat atgaatactt
     9421 tccgcagcct tagtcatgtt tttatattta cagacagttt gaaaatagta taaatagttt
     9481 atattcattt ggtattagtt cctttcattt acatattagt gacaagtata taattaacaa
     9541 tagcataagt tttgtatatt gttgtaaaaa aatagaatag ttttaaacta tcatagcata
     9601 agtttttgat attttcaatc tattaagaat agtgctatta ttaaattaac aaaagagaat
     9661 agctggtatg gtttaga
//
LOCUS       DAMJWS010000118         9584 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMJWS010000118 DAMJWS010000000
VERSION     DAMJWS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.123"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            491..1696
                     /locus_tag="PC399_003375"
     CDS             491..1696
                     /locus_tag="PC399_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2594203.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1767..1988)
                     /locus_tag="PC399_003376"
     CDS             complement(1767..1988)
                     /locus_tag="PC399_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594204.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2129..2728)
                     /gene="recR"
                     /locus_tag="PC399_003377"
     CDS             complement(2129..2728)
                     /gene="recR"
                     /locus_tag="PC399_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF2594205.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2741..3091)
                     /locus_tag="PC399_003378"
     CDS             complement(2741..3091)
                     /locus_tag="PC399_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF2594206.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3150..4787)
                     /gene="dnaX"
                     /locus_tag="PC399_003379"
     CDS             complement(3150..4787)
                     /gene="dnaX"
                     /locus_tag="PC399_003379"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF2594207.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4864..5128)
                     /gene="ffs"
                     /locus_tag="PC399_003380"
     ncRNA           complement(4864..5128)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC399_003380"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5166..5257)
                     /locus_tag="PC399_003381"
     tRNA            complement(5166..5257)
                     /locus_tag="PC399_003381"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5221..5223),aa:Ser,seq:gga)
     gene            complement(5258..5713)
                     /locus_tag="PC399_003382"
     CDS             complement(5258..5713)
                     /locus_tag="PC399_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF2594208.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(6034..7305)
                     /gene="serS"
                     /locus_tag="PC399_003383"
     CDS             complement(6034..7305)
                     /gene="serS"
                     /locus_tag="PC399_003383"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2594209.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7431..7636)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7764..8645)
                     /locus_tag="PC399_003384"
     CDS             complement(7764..8645)
                     /locus_tag="PC399_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2594210.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8657..9166)
                     /locus_tag="PC399_003385"
     CDS             complement(8657..9166)
                     /locus_tag="PC399_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF2594211.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            complement(9292..9408)
                     /gene="rrf"
                     /locus_tag="PC399_003386"
     rRNA            complement(9292..9408)
                     /gene="rrf"
                     /locus_tag="PC399_003386"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3057 a         1678 c         1030 g         3819 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taattttttt
      181 atttatcatg tattctttat ctttaatttt taaaacttaa tgctaagtaa acctgtttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatataaa tactatgcta cattttagga
      481 ggattttatt atgagcaaaa aagtattaat aatgtctgct tccactggtg gtgggcataa
      541 tagggctgct ctagctatta aagaagaact tacttccaaa acacttgatg gagagccaat
      601 tgaatgtgaa ataatagata gtttgaaatt agttaataat acaatggata aaataatttc
      661 aagaggatac gaaaaatctg ctatatatac tcccaaagcc tatggaagtg tatatagatt
      721 atctgaaaca aatcttttgt caaaaaatga atttaaagat aatctactaa taacattcat
      781 ggctaaaaaa tttaaaaagc ttattagaag cgaaaaacca gacttgatta taggaacgca
      841 tccttttcca atgattgctc taagtacatt gaaaaaaaac tttaatttac ataataatga
      901 atcaaatgct tacacagagc atttttataa acattataca aataccatta atgttcctcc
      961 gcttatttct gtattaaccg attatactac tcattctaca tggatacaaa atgaaattga
     1021 ttactatatt gttggtcatg aatatgtaaa agaattattg gtgtttgatg gagttgaacc
     1081 gagtaaaatt agaacatttg gaatccctgt tgaaaaatct tttctctctc acagagataa
     1141 agatattgtt ctttctgagt tgaatttatc tcctgataaa ttaactgttt tacttatggg
     1201 gggtagcttt ggggctggaa atataaaaga gacacttgat gaattactgg atactgatag
     1261 agactttcaa atacttgtaa ttactggtaa gaatgaaagc ctaaaagaaa aaatcgaaaa
     1321 aaaacttatg tctcgttatc atgataaaaa tgtatgtgtc cttggataca ctgataagat
     1381 gaatgatatt ttagcttcta tagatgtatt agtaagcaaa cctggtggac ttactacaac
     1441 tgaggctctt ttaaaagatg tacctatgat agttccttat tatataccag gtcaggaaga
     1501 agagaatctt gatttcttat ctaattgtgg tgcagcactt agaactacaa aaaaatataa
     1561 cttgtcaata ttactaaaag ttttaataga tgaccctagc agattagaaa tgctaaaaag
     1621 aaatattaaa tcaataagaa agtcaaattc agcccaaaat atagctaatt taattttaaa
     1681 tataatttct ggttgataaa ataaataaaa tccctatagt atatatatta tctattaggg
     1741 attttttatt ttctaattat ttaatattaa attggctcat aaaagtcttc ttcataatca
     1801 tactgataat ttttataatt atttctaaat tcagcatttt tatttcgtac atttttttta
     1861 gtttttttct tattagaaaa acttatatcc tcaaaatcat catatttagg tttatctttt
     1921 gatttcattt ttttaactct tttttctttc attgcattat gttcttctaa gttcataatt
     1981 ggtttcatgt tgcaccccta tcaaaatttt taaatgattt atacaacaaa ttatacaata
     2041 cagaagaaat ttattcaata aatctaaatc aaaattaatt tgaatttaac tcaaaaaact
     2101 acaaaaatcg aggtattaac ctcgattttt atatttctct tctgccttct agtgcttttg
     2161 atattgtgac ctcatctgca tactctaaat cgcctccaac aggaagacca tgagctatcc
     2221 gagtgacttt gattcccatt ggttttacta acctagcaat atacatggct gtagcttcac
     2281 cttcaatagt aggatttgtt gccattatta tttctttgac ttcctgattg ccaagtcttt
     2341 ggataagttc ctttattttt aacatatctg gtccaattcc atccattgga gaaattactc
     2401 catttaatac atgatactgt cctttaaact ctctcgttct ctccatagct gctacatctc
     2461 ttggatcttc cacaacacat ataacacttg aatctctact tttatttgaa cacatgctac
     2521 atgggtctgt atctgttata ttacaacaaa ttgagcaata tcttatttct ctttttgcat
     2581 ctataatcgc tttagataaa gcttccacat cattagtatt catattaata atatgaaaag
     2641 ccaatctttg agcagtcttt cttcctactc caggaagctt tgaaaattcc tctataagtc
     2701 tcgttatggg acctgtataa acttgcataa ataccaccta ctagaataac ccaggtatat
     2761 tcatacctcc agttacctta ctcatttgac ttgcttgtat atcatcaata tttctcaatg
     2821 cttgatttac tgcacttaaa actaaatctt gtagcatttc tatatcatct ggatcaacta
     2881 cttctggctt gatagttata tctataactt cttttttacc attaactttt acagtaactg
     2941 ctcctccacc aactgaagct tctacttctt tactttctaa ctcttgttga gctttttgca
     3001 tattttcttg cattttttga gcctgcttta acaagttgtt catatttcca ccaccaggca
     3061 tcattccgcc tccaaaacct tttttagcca taaacactat tcctcctaaa cttattttca
     3121 ttttattatt atatcataat agtattttat tatttactct cattttcatc aatactatct
     3181 tttatttcta atatctcttc actcactatg ttctttaata tttcctcacc aatgtccttt
     3241 ttctctttaa cttcaagatt tatatcttta acttctgatg tcagtactat ttttacatta
     3301 aaacttctat ttaaaacatc tcttattgtt ttttctaaat aatttattgt atcttgagag
     3361 ctcagtctag tttttgcaaa atcaaattta tcatcaaata taacatacaa catattagaa
     3421 tatacattaa aactttttac ttcacttaag agtgcgtata ttggcatttt tttatctttt
     3481 ttaatttgtt taagtatatt cttccaagat gactctataa gtataacatc ctcatttttt
     3541 acatcttcat aaactacatt ttcttgtttt acatctattt ctctatcgac ctcatactct
     3601 ttttctttat tattcccaat tttttcagac ttaaagttcc ccagttcaat cattttttct
     3661 aagttctcaa ctctttttat aagagcttct ttactttcat caaacattgg ttgagcaatt
     3721 ttcattatgg ttatttccac taaaaccctt ggatttgaag atgactttat atcatcctga
     3781 gttattgata atatatttaa aatccttatt aaatcattta tatctatatt tttagactga
     3841 attttaagtt gttctatagt ttcttctggt aatgaaatta tttcatccaa ttctgatgaa
     3901 accttacata ccattaaatt tctaaaatga tcaattaagt catttataag atttcttata
     3961 tctttgcccc ataagacaaa ttcatttaat atctcaagag actttttagt gtcctgctct
     4021 acaatacatt gagacaattc aaataattgt tctatattta cgcttcccat taattcaaca
     4081 acatctttat aatctatctt actttctcca aaagacatac attggtctaa tatactaagt
     4141 gcatctctaa gagctccttg agaatttcta gctattaaat taagagcttt atcatctata
     4201 tcaattcctt catcttcaca aatctttttc attctaaggg tcatatcttt aactgtaact
     4261 cttttaaaat cgtatctttg gcatcttgat aaaatggttg caggtatttt gtgaggctct
     4321 gtagttgcta atataaaaat tacatatgat ggaggttcct ctaaggtctt taataatgca
     4381 ttaaaagctc cttgagacaa catatgaact tcatctatta tatatacttt atatttagca
     4441 ttggctggag aatatttcac actttctcta agctctctta tatcatctac actattattt
     4501 gaagctgcat ctatttctac aacatccatt gtattatctt ttaatacaga ttcgcatatc
     4561 tcacactcat tacacggttc ttcatttatt ttattcaaac agtttactgc tcttgaaaaa
     4621 atctttgcag tagatgtttt accagtacct cttgttccac aaaacaaata agcatgtcct
     4681 atattatcac tatatatttg attctttaaa gtcttaataa tatgatcttg accaataaca
     4741 tcttcaaatt tttgtggtct atatgctcta tataatgctt tatgcatctt tttacctctc
     4801 tttagcaaaa ttaataaaaa aatccctcat tatctaaggg attagtcttt tatatattaa
     4861 atgctgtaca ccatctatcg acttaattcc ataggcgtta ccaaaacagt tagctcaact
     4921 taggcacccc cacggcacac aaaagtgatc acttaccgct gcttccttcc ggatctgacg
     4981 gggttcatga gctttcattg cgcaggacct aagcatcaac accacttatt tagggcggcc
     5041 ctacagaaaa taagccttta gatggaattc aatcctgcta tagcggattg caggttacag
     5101 ggcaccgcta cctccccatc tagtacagca aaattacaac catatttaat tactttcatt
     5161 gtttctggcg gagagagtgg gatttgaacc cacgatacag ttttacccat atactcgctt
     5221 tccaggcgag cgccttcgac cactcgacca tctctcctta atctcttttt ctcaatgcct
     5281 taaaaaattc ttgcaatatg atcgagcatt cttcttgcaa aacatcaaaa gctactttta
     5341 cattattatt attaaaataa tctaatttaa actcatgctg tttttctata tatgaatttt
     5401 ttacatgtct tgtccctatt ataagctttt ttattctaga attaactatt gcgccactac
     5461 acattatgca aggttccatt gtaacatata aatcacaatc agtcaacctc caaccaccta
     5521 atttttctga tgcttgtttt atcgctaaaa tttctgcatg tgccgtagag tcttttaacg
     5581 tttctgtaag gttatgagct ctagctatta tttgattatc tttaactata atagcaccta
     5641 ttggtgtttc ttttttatta tatgctttat atgcttcttt taatgcttct ttcatataaa
     5701 atgaactttc cataattcct cccaaaactt ataacaaact tatcttatca taattcattt
     5761 tataattcaa cttaatttta ttttgtttat ttaattatta ctatatatag ctatattttt
     5821 tttaaattag ttctaaaata atttttagat attcatacaa gagtgtttta taaaaatatt
     5881 tagtgtttta tgatgaaaat tctgctgtgt aaattaacat aaaaaaacta tgaaattaat
     5941 atttataata atttcatagt ttttttattt attgtttact cataatatat tctcttcatc
     6001 aaagcatact ctacatatac aattaatata aatttatttt ataacatcaa cacccatata
     6061 aggtcttaat tcttctggaa ctactacaga accatctgcc tgctgataat tttctaatat
     6121 agcagccaaa catcttccta tcgctaatcc agaaccattt aatgtatgaa catattctgc
     6181 ttttgatttt ttatcccttt taaatcttat tcctgctctt cttgcttgga aatcttcaaa
     6241 atttgaacaa gaagatattt ctacatatct attgtagctt ggcatccaaa cttctaaatc
     6301 atatttaaat gcagctgtaa atcccaaatc tcctgtacat atttttacaa ctctatatgg
     6361 aagacctaac atttgtaaca tagtttcagc atcatgagtt aatgattcta attcattata
     6421 agaatcttct ggtttaacaa atttaactaa ttcaacttta ttaaattgat gttgtctaac
     6481 taaacctctt gtatctcttc ctgctgaacc tgcttcagat ctaaaacaag gagtatatgc
     6541 acagtattta attggaagct ctgcaacatc taatatttca ttagcatgta tattagtaac
     6601 aggaacttca gctgttggta ttaagaaata ctctaatcct tctattttaa acatatcttc
     6661 ttcaaattta ggaagttgac cagtccctat aaaactattt ctattggcca taaatggagg
     6721 taagacttcc gtatagccat gcttagctgt atgagtattt aaaaagaaat tcattaaaga
     6781 tctttctaat ctagctccta atcccctata taaagtaaac cttgacccag ttattttccc
     6841 tgctctttca aaatctaaaa tacctagacc tgtacctata tcccaatgcg ctttatgctc
     6901 aaaatcaaat ttagtaggtt ctccccaagt tcttatttga acattgtctt catctgtttc
     6961 accttgtggt acctctggat gaggaacatt tggtattctc attaatgtat attccatttt
     7021 tgcttctact tcttttactt tctcatcaat accttttatc ttatctgata actctttcaa
     7081 tcttgccttt tcttctgtta catcttttcc ttcttttatt aattgaggta tttttttaga
     7141 atttacattt aactcatttt tcataacttc aacttcttga agtatttctc ttcttttatt
     7201 atctaattcc actacagcat caaggtcaaa ttctctttca cctcttcttt ccatagcttt
     7261 tttgatatca tctagatttt ctcttattct tttaatatct aacatattaa ttcctcctaa
     7321 taattttgat aatataagtt ataattccaa cttaatttac aaacattata cttgtaaact
     7381 cttagtttaa acaaatttta aaaataaaaa aagacctccg tttaccctat aaaagggacg
     7441 aaaatctttt atccgcgttg ccaccctaat tgactatact aaaatgtata atcctctcag
     7501 acctataacg taggtaaacg tctagcttta actagaagct ctagagtaga ttcaaaaacc
     7561 attgtaatga gttttcacca tccactcatt ctctgaatac aatctgtttt ttactatttt
     7621 ccttcacagc tttttaacta attgttttat ttgataatca ttatattata tatattaaca
     7681 tctttcaata ctattattca aattttttta gaattttctt taatttttta tattaaattc
     7741 attttttcta tttaattaag accttatctt ttatccattg tatttagatt acctgtatta
     7801 ttaaataata atgaagaagg aacaaatgga acttttatat cattctcatc aaatttattt
     7861 tttacctttt ccattacatc aaagtataca tcccagtaat catcagtttt tacccaaggt
     7921 ctagcaacta tttgcatggt gttatttcct atagagttta gacctattat aggctttgga
     7981 tcatttaata ttcttttttc gtttgtaaaa atttcattta atatagactt aactacatct
     8041 atatcactat catatgaaat attatatgaa aaatctattc ttcttttgtt ttgatgtgtg
     8101 taattaatta tattattact tgtcagttga aaattaggta taattatagt tttattatct
     8161 ggcgtcttaa gaacagtgct aaatatttgt atgtcagaaa ctgtaccttc tacacctgaa
     8221 ccttgaatat aatcacctat attaaatggc ttaaaaaata aaataatcat accagagcct
     8281 aaatttgata atatttcttt aaaagctaaa cctatactaa atccagcagc tcctaacaca
     8341 gctacaactg atgtagtttg tatccctata atacctaagc ttatcaatga tataatagat
     8401 aacatagata tatatattcc atatacaata aaattattta cacctttact cattccatat
     8461 ttgattagaa actttgaaac catccttctt actattttag atatataaat acctatcaca
     8521 aatacaatta ttgcatatat aatatttggt atattatcac taaaattatc gagcatattt
     8581 tctaaagttc tcttagttat attgctattt gttgaggcag tcttagccaa tgataaaaaa
     8641 ttcattcaac tcctccttat gataatattc cgttgtcctt taactctttt actatagctt
     8701 ctatagtttc ttcttttatt gcctcaattg tatgaatatg gctacattct gtcagaaccg
     8761 ataattgttt aaattcattt gtctcaactt tcttcataaa gttgtctata tccattttag
     8821 aataaatatt aagttctgca gttatctgac catatgttgg atgatttaca ataacatctt
     8881 ttataatacc acctaaatct acaattatct ttaattcatc ataaatttct ttagaatcct
     8941 tatgattttt acactttatt tgttttgtaa aatacggttg attatatatt atatatcctt
     9001 gaggtgtagc tattatttca aatcctctag ctcttataag ggctatatct tgaactatta
     9061 cttgcctact tacatttaat agttcagcta gtgttccacc ttttacagga tgcttagatt
     9121 cttttaaaat atctattaat ttacttcttc tttcattaga attcatatta caccttcttt
     9181 ccaaataaaa actttaccta tactatataa ttttatattt tatcttatta taaatcaata
     9241 ttgactttat attctattta atcttaactt ttataataaa taaaaaagac tacgtggcta
     9301 cgtcctactc tcccaggcag tttcccacca agtaccatca gcgctagaga gcttaacttc
     9361 tgtgttcgga atgggaacag gtgtatcctc tctgctattg taaccacata atctttatgc
     9421 ttaatatttc tattaagttt ttaataagtt tctagaatct agtaatacta gtttctttag
     9481 agttagcact ctaaaaacta cacatatatc ttaatgaatg aacatcaaat aaatattggt
     9541 caagtcctcg acctattagt atcgataagc taaatacatt gctg
//
LOCUS       DAMJWS010000119         9504 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMJWS010000119 DAMJWS010000000
VERSION     DAMJWS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.124"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(156..809)
                     /locus_tag="PC399_003387"
     CDS             complement(156..809)
                     /locus_tag="PC399_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2594212.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPIAEMKKEKLL"
     gene            complement(1054..2919)
                     /locus_tag="PC399_003388"
     CDS             complement(1054..2919)
                     /locus_tag="PC399_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HDF2594213.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2924..5344)
                     /locus_tag="PC399_003389"
     CDS             complement(2924..5344)
                     /locus_tag="PC399_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF2594214.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     TEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5358..6800)
                     /gene="glgA"
                     /locus_tag="PC399_003390"
     CDS             complement(5358..6800)
                     /gene="glgA"
                     /locus_tag="PC399_003390"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF2594215.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6829..7959)
                     /gene="glgD"
                     /locus_tag="PC399_003391"
     CDS             complement(6829..7959)
                     /gene="glgD"
                     /locus_tag="PC399_003391"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF2594216.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7961..9121)
                     /locus_tag="PC399_003392"
     CDS             complement(7961..9121)
                     /locus_tag="PC399_003392"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF2594217.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         3014 a         1723 c          924 g         3843 t
ORIGIN      
        1 tataagttca ttgatttata tatgttaatt taaatacatt attctatcgt agtgccttag
       61 agtttttatt tgtatttaaa gttaaattta ttcttgtttt tcctttaata tttaatagta
      121 aaacaacaaa attataaaaa aaataaagag aaactttata aaagcttctc ttttttcatc
      181 tctgctatag gtatcgtttt ccaatgttct ctcaaaattc tttcaacatt ttctaaatca
      241 ttctccctca tagattttac tacttcaata tgttgattat tgctgtatgt caacatttta
      301 tataactcat cttcattatc actagtatat gactttgaca taaaattata tcttaaagag
      361 ttcaatagat ttactaacac ctgattatct gatttagtta tgtatatatc gtgaaattca
      421 ttacttaatt ttaaatagct attgtaattt ttatacttta tagagaccgc catcatttct
      481 gttaattctt ccatttttac taggtctgat tcagttaaat tttgtaatgc tgaagaaccg
      541 gctaacgcat ccaatactcc aattacggaa taggtgtcta atttctcctt ttcctgaaat
      601 tcttttacta caaatccctt attaggtatt ttttctagta aattttcacc tgctaaccta
      661 gttaatgctt ctctcgctgg tgttctacta actccgatag atttacaaat ctcagattca
      721 gtaattctct gattagcttc atattctcca ttttggattt tctttgatat atagtcatat
      781 acttgagttg taagtggatc taccttcata agacgttcct ccatattaag attataaaag
      841 ttaaatctcc ctacaacttt ggtagagagt tcagttttat cttactaatt tatataatat
      901 cacaattatg atatttttta tacttctttg taatattttg aaatactaaa attcttatga
      961 taatttatac aaaaaaagta gaaaaagttt tgataaaaca aaactctttc cacattcgcc
     1021 ttattttcta tttatttttc taaaaaaaga tatttaattt actctaaaaa ttctaaatga
     1081 tttagagtct aaatctactt tcaactcatt attttcaatt tgtatagtac tatttgcatt
     1141 tttaagacta tactttatag gtatttcctc aataaaacta ctctctatac ataatgatat
     1201 actttccaca ttttcacttc tatttactaa aattaacatt ctatcattat tttcattaac
     1261 tctaataaaa gaaaatacag aatcattatt agtatatata aattctgtat ttccagaact
     1321 taaaagttta ttcttatttc taatttttat aacatctcta taaaaattaa gcatatcttc
     1381 gtcttcattt ttccatggat aagttcttct attatctgga tcaacacctc cacaaagacc
     1441 tgcttcatct ccataatata tatatggtac tccttcaaat gtcatctgta tagctacagc
     1501 aagcttcact aaatctacat catcatttaa ttccgtttta gctcttgtaa catcatgtgt
     1561 tccaattaaa tttaaattag acctaaatga ttcttttgga taattttctt taatctgcat
     1621 atatctatta caaagttcat atgaattaat ttctcctttt aagaaagaaa acatgttatt
     1681 tctaaaagga taacccataa cagaatctaa ttcttcacct aataaataac ttcttctctg
     1741 accatagcta atcttatttg atgcatcttc ccatacttca cctataagaa ttgagtcatc
     1801 atctgccttt tttaattcct tttttaactc tcttataaac ttagttggta attcgtctgc
     1861 aacatctagt ctccagccct taatacccat atttacccat ttatttataa cgctatcatt
     1921 gtcatatatt atataatcca tatagctatg ttctaattca tttatatttg gcaatgtctt
     1981 aacatcccac caacttttat atttttgagg aaattcttca aacatatacc atgaataata
     2041 tggagattct ttagattgat atgctcctaa actattatag tttccataca tattaaaata
     2101 tttactatct gctccagtat gactaaaaac tccatctaat actattgata tacctttttc
     2161 ttttgcttta tcaataagtt ctttaaatgt atcttcatct ccaaacattg ggtcaatttt
     2221 tttatagtca cctgtatcat acttatgatt actagaggct tcaaatatag ggcttagata
     2281 tagtatactt acacctaatt tttttaaata tccaagttta ttgattatcc ctcttaaatt
     2341 tccaccatgg aaatcccatc ttataacatc cccttgacta tcctttatgt acataggtgt
     2401 atcttcccaa tttccatata tgaaactatt ttttttagga ttatcaacct taccactacg
     2461 atttccatta ttgaatctat ctacaaatat atgataaagt attccttctt tgtaccatgt
     2521 tggtacttta aaatctttat aaacagtgat ttgatattta ttaatatcac tataattata
     2581 ttcacaagaa tatccattat ctctgttttt tccataaaat aaagtcttta tttgaccatc
     2641 catttcaaca tcaactttaa agtaataaaa ataaacacct gtaccatcaa acttttcaat
     2701 cttaccacaa aagcactttc caagtttatc attatctaat tcttcattta aagttattgt
     2761 tctcatttct tcttctctat ttatttctag ggaaatagac ttgatattgt atccctcatt
     2821 tactttaaca caaatggtca attcctcatc aaattttaaa gctccaaacg gagccttaaa
     2881 atttttatcc caagagttat attctattac actttgcatt taatcacacc cttttaaacc
     2941 aaatgtcttt tgcatactca gaaattgttc tatcagaaga aaaaaatcca gagttagcta
     3001 tattaactaa agacatttta ttccattttt ctttatctct atataacctg tttatatctg
     3061 cttgagcttg accataattt tcaaaatctc tcaatacaaa atactcatca ttgtaagtag
     3121 tgagtgaatt ataaatactt cttccttctt cgccaagatt aggaataaat ccatttatta
     3181 aatcatctac tacccttttt atatccatat tagaatgata taggtcaagt gaagaatatc
     3241 caccatattt attataattt agtacttgtt ctgcactaag tccaaataaa aacatatttt
     3301 ctttaccaac ctgttcacat atctctacat ttgcaccgtc aagtgtagcc aaagttatag
     3361 caccattcat cataaacttc atattacttg ttccagatgc ttccttagtt gtagttgaaa
     3421 tttgctcact aacatttgct gctggtatta ttatttctgc taatgatact ccataattct
     3481 ctaaaaatac tacttttaac ttgtccttta ctctaacatc attatttata gtacttgcaa
     3541 cagaatttat aaatttaatt atacatttag ccaaataata tcctggtgct gcttttgcgc
     3601 caaatataaa agttcttggg tcaagattca aattaggatt atcaagtaat tcatgataca
     3661 tatgaagtac attaaatata ttcagtaact gtcttttata cgcatgcaat ctcttaactt
     3721 gaacatcaaa tatcgaactt gggtctacat ttaaatcgta tttatctttt ataaactttg
     3781 ctagatttgc tttattattg tattttatat catcaagtct ttgtaaaaca gaggaatcat
     3841 ttttaaattt ttcaatattt tttaattgta atgtatcttt cttccatgaa tcaccaatta
     3901 aatctgtaat taaattgcta agttgaggat ttgaacttat aagccatcgc ctatgtgcaa
     3961 ttccattagt cttattatta aatttatttg gttcatcttg atagaaatct tttaatacct
     4021 ctgtttcaag aatatgagta tgaagcttag caactccatt tacactatga cttgtgacaa
     4081 tacataagtt tgccatattg atattgtcac agtctattat actcattctt tttattctat
     4141 cttggtcata tcctttatta tttaattcct ctacatatct tctatttatt tcttctatta
     4201 tcatataaat tcttggtaat agctctttca tcatacttac aggccatttt tccattgctt
     4261 ctgacattat agtatgatta gtataggaca ctgttttttt agttatttcc catgcttcat
     4321 cccaagataa attttcttca tcaagcaaaa ttctcataag ttctggtatg cataaggtag
     4381 gatgtgtatc atttatatga attgctacct tatcgaataa attattaata tttaatttgt
     4441 tctttttata ttttcttatt atatcttgta tccctgcact tacaaaaaag tattcttgtt
     4501 tcaacctcaa caacttacct gcatagtttg aatcatcagg atataatacc tgtgatattt
     4561 cctcagtata atacttatgt tttaaagcct cttcatagct accagaataa tttagagcat
     4621 ttgaggtaag ttcaccaaaa tctctctttg gtatttcact cttaaacaat cttaaagtat
     4681 ttatacattg gttttggtat ccgattattg gtatatcata aggcattgcc attacaggaa
     4741 ggtagttttt atgaattact tttaaatgac taccttcctt gattaattct acctcaccac
     4801 caaacttcac cattgttgct tcatttggtc ttacagtttc ccatgcatat cgtccttctg
     4861 taagccagtt atcaggcact tctacctgat aaccatttac aaatttttgt tcaaacagac
     4921 catatttata ccttattcca tatccttgac cacttatgtt caaagatgcc atagaatcta
     4981 aaaagcaagc tgctaatcta ccaagtccac catttcctaa ggctgggtct acttcagttt
     5041 caattaaatc atccaagttg attcctaatt cagataaacc ttctctaact tcttcttcaa
     5101 tatttaaatt taataaatta gattttaact gtctccctat taaaaattct aaactaaaat
     5161 aataaacttc ttttttctta tctaactttg tagcaaccca tttttttgct atctcatctt
     5221 tcaatagact acataataca tttaacaatt gttcatcagt agcttccttg atagattgtg
     5281 catataagct atacattttt acttcaaatt tttgcttgaa tttctttttg cttattgtta
     5341 ccaaaaaaac cactccttta atcacatatg tctctatatg tttctatata ctccttagct
     5401 gattttttcc aactattatc tgttttcatt gcattttcaa ctaatttaat ccatttttct
     5461 ttgtccttaa atactttgat tgcatttttc aaacagaaaa acatctcatg agcattgtaa
     5521 tttttaaaac taaatccatt gccttctcca gtaaatttat tataaggtgt tactgtatct
     5581 ctaagccctc cagtctctct aactattggt aaagaaccat atctcatagc taacatttga
     5641 cctataccac aaggctcaaa taatgatggc attaagaaca tatcacttgc agcatatatc
     5701 tgttgagcca atgatgcatt aaaagtaatt ctagctgata ctttatctga aaaattagaa
     5761 tcgtaatagt tgaacattga ttgatattga tgttctccag ttcctaaaac gacaatctgt
     5821 aaatcttctc tcataagttc tggcatcatg tatgctatta aatcaaatcc tttttgagaa
     5881 actaatctcg aaactatacc tatcaacgga atagatgaat cttttttaag tcccaatata
     5941 tcttgtaatc tcaatttatt ttctactttt ttgtcaattg agtctacatc ataatgaaca
     6001 aatatatctt tatcttttgc agggtcattt aaatcataat ctataccatt taatatacct
     6061 tttaattttc ctgactcttt tctaagtaat ccatctaatt gttcaccata aaaagatgtt
     6121 tgtatttcat ttgcataagt aggactaaca gttataattt tatctgcaaa atttatacca
     6181 gctttcataa aatttattga gccataatac tctatatctc cattatcaaa gtatttttgt
     6241 ggtaagtcca atatatcata taaaacatgc ttatcaaata caccttgata ttggagatta
     6301 tgtatagtat acatagtttt tatattttta tacccctcaa gggttgcata tctttctttt
     6361 agaattaatg gtaacatccc tgtatgccaa tcatttatat gtattacatc tggataaaaa
     6421 tcaattctac ttatagcttc taaaactgca tctgtaaaga aagtatatct ttctgcatca
     6481 tctccatatc catagatact ctttctttca tcttctcttc taaaataaaa ttcattatct
     6541 ataaagtaaa attttacacc atctaattcc atttctaaaa gaccacagta ttgatttctc
     6601 catccaacac gtacactaaa cacagctatt tcttttagtt gatcttttaa ataacttggt
     6661 atagttgaat atttgggcat tattaccctt acatctacac cttctttttt taattcttta
     6721 ggcaatgcat aagaaacatc tcctaatcct ccagtttttg caaagggcca acactctgct
     6781 gttacataaa aaaccttcat tagtcattcc cccttctaaa aaatttattt atatatttgt
     6841 attcttttcc actacaagag gatatttttc atctcctctt aattctttcc catttgatac
     6901 actcgtattt ttatctaata ttgcattata tagttttgca tttgacttta ttacacaatt
     6961 ttgcattact attgagttct ctataactgt tcctttttca acatttacct ttctaaatat
     7021 gatactatct tttacagtac cttctattaa acaaccagat gctataaagc tattttctac
     7081 tttagcacta tctgtataaa ttgtaggtga ttcattcttt tcttttgtaa atatctttct
     7141 ttcagaatat aataattcat ctgcaacttc taaatttaat aaatctttgc tcatttcaaa
     7201 atatgattca acagaattta cacattttag atatcctgta tattcatagg ctcctatttt
     7261 catattttct gcttcttcaa taataaattc ttctaaataa caataattgc ctttatttac
     7321 aatgttatat atacattcaa caaaatcttc acgcttcata atatatgtct ccatacatat
     7381 gtttgctcta gggaacttac ctaaattatt tcctacattt attaccctat tgttactatc
     7441 taaatttaga gttaagttac cagcaaaatc tttatttgca ttatttacat ttttatatat
     7501 tatagttata tcattatgag attttttatg gtattccaat gcttgagaat aatttaaatt
     7561 gcatagcata tagcttggag ctattaaaat atattcttct tcactctttt ctatataatc
     7621 tagattagct aagatagtat atatatcacc ttctctatgt gagtaagtat ttctatactt
     7681 agtgttttct ggactaaaaa ttgataggcc accttttttt ctacttaaat cccaatcttt
     7741 accactacct atatggtctg ttaatgatct gtatttttct tttgcgaata ttccaacttt
     7801 agtgattcct gaatttatca tattagataa agcaaagtct attattctat atcttccagc
     7861 tattggtgta gatgctattg gtcttccata gttaagtttg tttattgctg ggtctccttt
     7921 tttgtttaaa tttattatcc ctaaacattc atttctcatc ttaaagacct ccttctatta
     7981 atacgcatct aggttcgata ttttcatact cagaaactac atttatttca ttatttacat
     8041 tctttatctg aacattatca tttatagttg cacactcacc tatcatagct ttttctatag
     8101 ttacattttc acctattaca acatttggca ttattactga atcaattact ttacttcctt
     8161 taccaacaac aactccatgt gataatacag agttacctac ttcacctaaa attctacaac
     8221 catcagctat cattgatcta tgaacatttg catttttacc tatatattgt ggaggcattg
     8281 ccatagtatt tgtatatatc ttccacttag ggtctgctaa atcaagtgtt tctggagatt
     8341 ttattatatc catattagca tcccatagac tttgtatagt tcctacatct ctccagtatc
     8401 ctttaaatgg ataagcatac attccaacat tatcttctaa cattttaggt attaaatttt
     8461 ttccaaaatc atcataattt atttctggat ttttttctgc ttccttaaaa taatttctca
     8521 acattttcca atcaaatatg tatactccca tagatgccaa attgctctta ggttcacttg
     8581 gtttttcttc aaattcatag atacttgaat cttcatttgt attcattatc ccaaatcttg
     8641 aagcttcatc ccaaggaact tcaatcactg ctattgtagc tttagagcct ttttccttat
     8701 gaaattttaa cattttgcta taatccatct tgtatatatg gtcaccagat aatatcaata
     8761 catattctgg attataggta tctacaaagt ccatattttg atatatcgca tgtgctgtac
     8821 cattatacca atttccttct ttttcattca taaatggttg taaaacatag acaccaccat
     8881 ttattctgtc caaatcccaa tgacttccca tgcctatatg tgaatttaat atcaacggtc
     8941 tatattgagt taaaacacct acagtatcaa taccagagtt tgaacaattg cttaacacaa
     9001 aatctataat tctatactta cctccaaatg atacagctgg tttggcaatt ctttttgtaa
     9061 aaacacctaa tcttgaacct tgccctcctg ccaaaatcat agctaacatc tcttttttca
     9121 tttaatgaca ctcccctcat ctagtaatta atatttataa ttgcattttt acttttataa
     9181 actttatcag tcaataaatg tcgtgtataa atgttgctta aataatacct taataaaaat
     9241 aaaataatat caactataca attgatataa tgtatacccc ataatggact taccgacgta
     9301 tacattattt tccataagca ctatagtatt tattaatatt caattattgg ttaaaaaata
     9361 atacaaattt tattttataa atataaaaac attttttata aatcctttgt gtcatatgaa
     9421 catgcttatt ttttaatttg ttaaattcgt aatagcaaaa tataatctaa tatttgttaa
     9481 ataattatcc aaagaaaatc aagt
//
LOCUS       DAMJWS010000120         8888 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMJWS010000120 DAMJWS010000000
VERSION     DAMJWS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.125"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(343..1239)
                     /locus_tag="PC399_003393"
     CDS             complement(343..1239)
                     /locus_tag="PC399_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2594218.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1380..2807
                     /locus_tag="PC399_003394"
     CDS             1380..2807
                     /locus_tag="PC399_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2594219.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIAKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFNWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(3003..5597)
                     /gene="clpB"
                     /locus_tag="PC399_003395"
     CDS             complement(3003..5597)
                     /gene="clpB"
                     /locus_tag="PC399_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF2594220.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5634..6230)
                     /locus_tag="PC399_003396"
     CDS             complement(5634..6230)
                     /locus_tag="PC399_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594221.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6247..6651)
                     /locus_tag="PC399_003397"
     CDS             complement(6247..6651)
                     /locus_tag="PC399_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2594222.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            7018..7392
                     /locus_tag="PC399_003398"
     CDS             7018..7392
                     /locus_tag="PC399_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2594223.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7389..8252
                     /locus_tag="PC399_003399"
     CDS             7389..8252
                     /locus_tag="PC399_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2594224.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            8252..>8888
                     /locus_tag="PC399_003400"
     CDS             8252..>8888
                     /locus_tag="PC399_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2594225.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         2976 a         1312 c         1023 g         3577 t
ORIGIN      
        1 ctttaggatg gatttaaatt ttctataaaa atcatactat tttagctgtt tacttgctta
       61 tttaatagtt ttatttcttt gattaaatat ctttcagaat ctaagccaga tgtctcaaaa
      121 aaactgattt ataatataat cattttttct gctattctta tttttccaat tttagtttca
      181 tattaaaata tacttttcat agacactaca ttctttcata ctcattttca catttaacta
      241 tacaagtagt cttactcatt tatatcctat gctttattaa tatttattaa gaaaaattca
      301 aagatttaaa aatatatatt ttctataaag tatatttatg aattatttca tatcttcttt
      361 taaaataaca ttactaactt ctctatgttc atttctatac ttagttggag atatattata
      421 attcttttta aataagcgac taaaataatt tatgctatca aagccacatc tcatagcaat
      481 ttctgtcata tttaaatctg tttcctttaa ataatatata gctttatcaa gcctgattcc
      541 atttataaaa tcgattgtag tcttacctgt tatttgctta aatgttctac aaaagtgatg
      601 tgcactcaag ttcacacgac tagcaagttc ttttaatgta attttatcca tatatttttc
      661 ttctatcaaa tcaaagatac attcaaattt ttctaaagaa tactttcttt ttataaattc
      721 atcttcagaa tatattttat caatatgact tcttaataag agtactatta attgatagat
      781 attagctttt atagataact catatccaac ttctttattg tgatattcat caagtatagt
      841 tgtaatacat tttaaaatca gagaatcatt ttgaattaaa ttattaaata caatatgatt
      901 taaagccaat ggtgctaagt acttggtttg taacaaatca acttgattac taaaaaggaa
      961 tgtgggttca atacgaatac tatagaattt taaattatct gataagcttt ctaaataatg
     1021 taattcatta ctgtttgcta ttacaatatt tccaggcact accttaaaat tatccaatcc
     1081 acattcaaga tatgctatac catcaataaa ataatatatt tgtagatgct catgccaatg
     1141 tcttttaaag tttgctcctt ttttaatgtc atcctttaaa aaaatatcta taaagttttc
     1201 ttgacctttc ctgttaaaat atgtattttt atccttcatt attcccccct catcaaaata
     1261 gtgctaatta taagcaatat agttattgta ttcattattt catatcgcta ttatataaaa
     1321 tgtaactatc attgtaaaca ttaacataat gcagtcatta taataaggag gtaataaaaa
     1381 tgacaaactc ttttaaaaaa gatgataata gaaaaaaaat cacagtttta tttgtagtca
     1441 ttgccatgac ttttatggca acattagatt ctagtattat aaatgttgct ctcccagttc
     1501 ttgcaagcaa actaaatgta tctctagcat ctattgaatg ggtaatagca agttactcaa
     1561 ttattatttg ttcaacacta ttattttttg gaagacttgg agatattatt gctaaatcaa
     1621 gagtatttca agtaggtact attctcttta catcagcttc attactatgt ggattaagta
     1681 attctttaac tttacttata gtttgccgct ttattcaagg aataggcgct tctgcctaca
     1741 tggcaaacaa tcatggaatt atcacagaac tatttccaaa agaaagcaga ggaaaagccc
     1801 tagggatttt agttactgca gttgctattg gaaatatggt tggaccatct gtgggaggat
     1861 ttattttgtc aatatttaat tggaatgtta tattttttat aaatattcca atcggtctta
     1921 ttgtaatctt tttaaataca aaatttcttc ctaatagtaa aaaatcttca gaaaatatgg
     1981 ataaaactgg tgctatactt caatttttag gaacaacatt attttttagt gctctaatta
     2041 gtgctcaaca aacaggatta ctaaatccat atatattaat tgcactatta ttatcaatta
     2101 tttttattat attatttttg atattagaaa aaaaacatcc tcaaccgctt cttgaccttg
     2161 aaatttttag aaattttaaa ttttcattga atttaatttg tgctcttaca tcattcattt
     2221 gtattgcatc atcaagtatt ttaattccat tttatttaca aagtactatg aaacttcccc
     2281 ctattcaagc aggtttattt atgatactat cacctttgat tttagccatt ttttcaccta
     2341 tatttgggaa tatatcagat aaaattaagt cagaaaaaat tattttaatt ggtcttctcg
     2401 ttatgagttt tggattcttt ctaatgtcaa gattgaaaga aagttctgcc cttattttat
     2461 ttgtaattta tatatcgata atatcaatag gacaagctat atttcaacct gctaacaatg
     2521 ctttgataat gtcaagctgt tctagaagta agctaggagt tgtaggaagc ataaattccc
     2581 ttgtaagaaa tttaggacaa gttataggta ttactatttc tacaacactt ttatataatt
     2641 ttatgagtat taaagctgga tatagggtca atgattatgt gattaataat gataaaatat
     2701 ttgtatttgg aatgagaaat gtttatatta ttgttacact tgtttgctta ataggtgcaa
     2761 tattaattgg cttttattta tttaaatata ataaaaatga acaatagttt ttataattat
     2821 attcaaatta tgattatatt ataagaaatt tactaaaata cttcctttga gttaaattta
     2881 atttggacat attgacttaa aaatgtttta ttaatttgat aaaatatttc ttttcctata
     2941 gtttataaat aaaaatcagc tgagcaaaat atttagtata ttatgctcaa ctgattcttt
     3001 tattatttaa ctaaaacttc aatattttca tcttttccat ctataactat agtacatcca
     3061 ttatacacat ctccagcaat aagtttcttt gcaattatag tttctaatgt atttcctata
     3121 tatctcttaa gaggtcttgc tccatataca gggtcatatc cttctctcac cataacatct
     3181 tttgctgaat ttgtaacttc aatagtaata tctttttctt ttaatctatt ttttaaatct
     3241 ttcataaata tatctataat tttcttgata ttttcttggt ctaaaggctt aaacattata
     3301 atatcatcta ctctgtttaa aaactcaggt ttaaatctat gcttcatttc attcattact
     3361 aaattgttag tagtctctgt tatatttcct cctgcttcta atagataatt acttcctata
     3421 ttagaagtca ttattataag agtattttta aagtctactg tcttaccttt attatcagta
     3481 agtctaccat catctaatat ctgtaagaac atgttaaata catcctcatg tgctttttct
     3541 atctcatcaa atagtattac agaataagga gcacgtctaa ctgcttctgt aagttgacca
     3601 ccctcttcat atcccacgta ccctggagga ggtccaacta gtcttgatac agcatgtttt
     3661 tccatgtatt cagacatatc tattcttatt atattatctt cactatcgaa taaatttcta
     3721 gcaagagttt ttgcaagttc agttttacca acacctgtag ggcctaagaa aataaatgaa
     3781 ccaattggtt ttctttcatc ttttaatcct gctcttgcac gtattactgc atttgaaact
     3841 gctgtaactg cttcatcttg tccaataact cttttatgaa gttcatcttc tagtttaagt
     3901 aatttttctc tttctccttc aactagttta gttactggta tacctgtcca ctttgataca
     3961 atgctagata tttcttcttc agtaacttct tcttttaaaa gagaattttc agaaccatca
     4021 ttcatatttt cttcgtactg ctttatttgt tcttctaact taggtatttc accatattta
     4081 acctctgctg ctttgttgaa atcatattct ctttcatact tttcagcttt acctttagct
     4141 tcgtctaatt gagcctttaa gtttttaata tctaatattt gactcttttc tttttcatat
     4201 tttgctgtca tttcatcatt tttagatttt aactctgcta tttctttttg aatatcatcc
     4261 aatcttgatt tacttttatc atcattttct ttaagtaatg cttctctttc agtttctaat
     4321 gtaaataact ttcttctaac aacatctaat tctgttggca atgaatctat ttcactacga
     4381 atcatagctc cagcttcatc aattaaatca attgctttat ctggtaaaaa tctatcttgt
     4441 atatatctat gagaaagttt tgctgctgct acaatagcat tatcatgtat tcttactcca
     4501 tgatgtattt caaatctttc ttttaaacct ctaagtatag atattgtatc ttcaacagta
     4561 ggttcttctg caattacagg ctgaaaacgt ctttcaagag ccttatcttt ttctatatac
     4621 tttctatatt catcaaaagt agttgcacca atacagttca attcacctct tgcaagcata
     4681 ggtttaataa gattacctgc atccattgaa ccttctgttt taccagctcc aacaatagta
     4741 tgaatctcat ctataaacag tataattttt ccatcagaac tttgaacttc ttttaaaact
     4801 gcctttaatc tttcttcaaa ttcacctcta tattttgcac cagcaattaa agctcccata
     4861 tcaagtgaga atacaatctt atcttttaaa ccttctggga catctcctct tactattctc
     4921 tctgctaacc cttctacaat tgcagtttta cctacacctg gctcaccaat caatacagga
     4981 ttattttttg ttcttcttga taaaattctt ataatccttc ttatttcttc atctctccca
     5041 attacagggt ctaatttatt tttcttggct aaatctacta agtttgttcc atatctagca
     5101 agtgcatcat atgtgccttc tgggtcttgt gtttctactc tttgactacc tctcacttgt
     5161 gataaaatat ttaaaaagtc attcttattt atattatatt gttttaatat tttaccaaca
     5221 gctgattttg attctgtttc catcattgca agcataacat gttctacact tatatatgaa
     5281 tctttaaatt ctttagaaat actctctgcc tttataagca cctcatttat ttttctagta
     5341 gctgatactc cttgtgagtc agcaccttct ccatatactt ttggaagctt atcaatttca
     5401 aagtttacag tatttctcaa agaatcaatt gatatattca ttttttcgat tatatttggt
     5461 atcaatccat cctcttgatt aataagtgct gagaaaagat gaattacatc tacttgttgg
     5521 ttatgatttt ttactgcttc gttataagcc tcatttaaac ttttttgaac tcttaaagtc
     5581 attttttcta cgtccatata aatccctcca ttttaatatt atatccaaat aaattaaatt
     5641 ctctcattta taattattga ttttctaagc tctaaaagac tatcaagtaa attctcgttt
     5701 ttttgtatat cgaaactcaa tgttaaatta aatgttccat ctgaattaaa aaaatcgaat
     5761 acttcttttg attttgctat taaatacaag tcttcatcta taagtctttt ttcacctatt
     5821 aataaatact gacatttatc tataaaactc tttttaaaat caatagtgta tagattttct
     5881 aaaatattat attgataaat agcaatatta ttaaacactt ttaatatgtt tgaatttaca
     5941 aaattgtaaa tttgattatt gttatacagt gatatttgtt cgtatatata ttccctaaat
     6001 aaaggatgaa tgttttgctt aagttttaaa gtaacttcag ctggtatttc aaaataaagc
     6061 ttccagtact tagagttatt tttttctaaa ataaaagcta ctttgttcaa actggtagaa
     6121 ctaatctcct ttagtgtaca attaaaatta tagtgtctca aactagtttt ttcattctca
     6181 aatacatagt caaaccactg tagaacattt ctatcaagtt ttaaactcat ataaaaacct
     6241 tcttccttat ttactaacct tattaggtct ttttatctga ttatattctg ctaataaact
     6301 ttctttatca gctctactac ctttatatgc tatatgtgga tttatcatat actcaccatt
     6361 tttcactctt tgtaaaaaat ccattttttt cagctccgta aacaatgaag ataaagaagt
     6421 ttttggatat tcaaattcac aagataattc atataatgtt tttctaatct tattattttt
     6481 atccatagag tttattaatt ttaatggaat taaatccctc ttagtataca ttccttctac
     6541 tactttaata taattcttaa aacaaacatt catatactta cttttattca taactaaact
     6601 tttgctctct gtatattcct gatttaaatc cttttttaat gtcttatcca aattaaatat
     6661 ctcttctcac tctccttatc tctaattgat aatataaata tattacaaaa aaatatgtat
     6721 gtcaaaactg aaaatgcaaa aagttcacct tataagtaaa cttttattta gctataagct
     6781 aaactttatt atttttttaa tttagtattt ccaatttatt ggcaggattt ttgaacaata
     6841 tttataaatt ttttaaaaaa atttaaatct atttaatatg gcagactttc ataaaataca
     6901 ttacatttat taaatgtata atattggcaa ttagtttttt ctaaaatcag ttgacatatt
     6961 atatatatat ttatataatg tgtatatact atatatacaa tttaaggagg tgtcttattg
     7021 aatatagtta ttagtaattc gagtgggaag ccaatatatg aacagattac tacacaaatt
     7081 aaaagtatga ttataagtgg agagttacca gaaggctcag ctcttccaag tatgagacta
     7141 ttggcaaaag aacttagaat aagtgtaatt actacaaaac gagcatatag cgatttagaa
     7201 cgagacggat ttattgagac agttactggt aaaggcagtt ttgtatctag taaaaacatg
     7261 gattttgtca aagaagaaca atttcgttta attgaagaat acctacaaaa agcagttgat
     7321 acagcaaaaa gtagtgatat tacatatgac gaattaaaag atattttatt tttattatat
     7381 aaaggagaat gataagtatg gatgcaataa ctataaaaaa tttaaataaa acttataaag
     7441 attttagcct acaagatata tctttttcag taccaaaagg gagtgtaatg ggatttgtag
     7501 gtgaaaatgg tgcaggaaaa acaactacat taaaagcaat tttaaattta atatcttatg
     7561 atagtggaaa tatagaaatc tttggtcttg atagtaaaaa aaatgaaaaa gaaattaaag
     7621 agcaaattgg ggttgtattt gagggaagca actttcatga aaatttaaac actgaccatg
     7681 tttcaaaaat catgagtaaa atctataaaa attggaatga cacattattt aaagactact
     7741 taaaaaaatt gagagtacca gacaataaat taattaagga gttttcaaag gggaataaga
     7801 tgaaacttag tattgctgta gccttatctc ataaaccaaa attattaata ctagatgagg
     7861 caactagttc acttgaccct atcgttcgtg aagaaatact agatatattt ttagatttta
     7921 tacaagatga agaacactct attatcttat cttcacatat tacaagtgat ttagataaga
     7981 tagcagacta tattacattt atacataaag gaaaaattgt atttagtgaa aataaggatg
     8041 aattaattga tactatggga gttttaaaat gtaaacctag tgattttgat aacctttcaa
     8101 gagaggatta ttcatattat cgtaaaagcc aatttggata tgaagtactt cttaaagata
     8161 agcatagatt tatctcaaga catccaaact gtattgttga taatacgtct atagaagaaa
     8221 ttatgttatt ttatgtgaga ggtgataaat aatgaaaggt ctaattttaa aagatttact
     8281 taatttgaaa ggaaatataa agtttatact tttattcatc attatgtttg gctttatgtc
     8341 ttctttagga gatggaaatg ttaataactt tataggtgtt attatagttt tatgtactac
     8401 aatgattgtt tcaacatttt catatgatga tttgaataaa tgggatagtt atgtacttac
     8461 aatgcctatt aatcgcaatg atattgtatt aagtaaatat ttaactatgt taatatttag
     8521 ctttataggt gttcttgttt cacttattgt tagtgtaact attggttatt ttaaaaatac
     8581 acttatatta aatgaaacac tacttattaa tgcactaatc ttatcaattt cagtatgttt
     8641 tggtagcctt attctaccac ttatttataa atttggtaca gaaagagctc gtttattaat
     8701 gattttatgc tttttagttc caacactagc tttattagtt tttaaaagta ttttagaaaa
     8761 tattagttca cctatttcta tagaaattat acttaataca ctagtatatt ctcttccatt
     8821 tgttgctatt ttactatttg tgatttcata ttttatatca tcaaaaatct atagtaaaaa
     8881 agaagtat
//
LOCUS       DAMJWS010000121         8851 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMJWS010000121 DAMJWS010000000
VERSION     DAMJWS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.126"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            138..488
                     /locus_tag="PC399_003401"
     CDS             138..488
                     /locus_tag="PC399_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2594226.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            507..2150
                     /locus_tag="PC399_003402"
     CDS             507..2150
                     /locus_tag="PC399_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF2594227.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2231..4288
                     /gene="recG"
                     /locus_tag="PC399_003403"
     CDS             2231..4288
                     /gene="recG"
                     /locus_tag="PC399_003403"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF2594228.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4347..4901
                     /gene="rsmD"
                     /locus_tag="PC399_003404"
     CDS             4347..4901
                     /gene="rsmD"
                     /locus_tag="PC399_003404"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF2594229.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4903..5400
                     /gene="coaD"
                     /locus_tag="PC399_003405"
     CDS             4903..5400
                     /gene="coaD"
                     /locus_tag="PC399_003405"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF2594230.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5404..5928
                     /locus_tag="PC399_003406"
     CDS             5404..5928
                     /locus_tag="PC399_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594231.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6272..8191
                     /locus_tag="PC399_003407"
     CDS             6272..8191
                     /locus_tag="PC399_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2594232.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSIIELINKQN"
     gene            8204..8491
                     /locus_tag="PC399_003408"
     CDS             8204..8491
                     /locus_tag="PC399_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2594233.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
BASE COUNT         3674 a          915 c         1642 g         2620 t
ORIGIN      
        1 ccaaaaactt ttctaggttt aaattatacc atatttatag tatgatatga ttataatttt
       61 atataataaa taagtaaaaa tagtaaaaaa taaattaaga aatgttttta ataaaaataa
      121 gcttaagggg gtcaaccatg agtgcaaagg taacgaatca atatggaaat atagaaatag
      181 ataatcaagt aatagctcaa gtcgtatata gagctgcaat ggaaagttat ggcttagtag
      241 gtttagcata taagtctaaa ggaatagtag agctattaaa aggcgagaac gcaactaaag
      301 gtgtaagagt tgaggaagta gaagaagatg ccatagcaat cgaactttat gttataatac
      361 aatatggtac aaatatatct actgttgcca ataatataat agatagagta aaatatgtag
      421 ttgaaaaaat gactggcgta agagttacta gaattgatat taatgtacaa ggaataagag
      481 ttaagtaatt tcataggagg aaacaaatga ttcaatatat agatggtaaa aggttaaggg
      541 agatgtttat ttcaggtgca aacaatctcc aaaacaataa agagttagtt gacaagttaa
      601 atgtattccc tgtaccagat ggagatacag gaactaatat gtcattaaca atatcttatg
      661 ccttaaaaga attagcaaaa gttgaaaatg ataatatatc agatatcgga aaagcattat
      721 ctaaaggttc tttaatggga gctagaggaa actcaggagt tatactttca caaataatca
      781 gaggtatagc taaatctata gaaggaaaaa gtaaattatc aacagaagat ttagcaaaag
      841 cttttaaaaa tggttcagac acagcatata aagctgtaat aaaaccaatt gaaggaacta
      901 tacttactgt agttagagaa agtggagagt ttgcaataaa aactgcgaaa aaagagaaag
      961 atgtagtaaa gtttttaagt atgcttgtga aggaatcaaa ttcttcatta gaaagaactc
     1021 cagaccttct aaagaatcta aaagaagctg gtgtggttga ctctggtgga aagggtcttg
     1081 tacttatata tgaaggaatg ttagcttcta taaaaggcga taacatagaa ataaaaaatg
     1141 ttgacctaga tactaatatt tcaacaagta tggattttgc aaagagtact actagcacag
     1201 atgatataaa atattgctat tgtacagaat ttattttaga aagttctaag gtagaagata
     1261 ctaaaataag agacataatg atggcttatg gtgatagcct agcagtagtt ggagatgatg
     1321 gagtcataaa ggtacatgta cacactaatg acccaggaaa tgtacttcaa gaagctctaa
     1381 agtacggtca attattaact ataaaaatag aaaatatgaa gttacaacat gaaaatacat
     1441 tactggatgt agaagaaaag aaagaaaatg atagtgaacc tttagaagaa gaaaaagagt
     1501 ttgggtttat agctacttct atgggtgaag gtttggcaaa tatatttaaa gatttcggag
     1561 ttgaccatat aatagaaggt ggtcaaacta tgaatccaag tactgaagat tttatgaatg
     1621 ctataaagca tataaatgct aaaaatatat ttattttccc aaataacagc aatataataa
     1681 tggctgctaa tcaggcaaaa gaattgagtg ataaaaatat aatagttata cctacaaaaa
     1741 atacgcctca aggatttgct gcattagtaa cttttaatgg agaattaagc gaagatgaaa
     1801 ataaagaagc tatgatgaat gctttaaact cagttaaatc aggtcaagta acttttgctg
     1861 ttagggatac agtgatgaac gaaattgatg ttaaagaagg taatataatt ggaattgcag
     1921 aagggaattt attatcagca ggagattatg tggatgaagt aacatcaaac cttattgaaa
     1981 agttggttga tgaagatact gctataataa cattattctt tggtgaagat gttacagaat
     2041 ctcaagcaaa tgaactacgt acttctttag aagaaaagtt tgaagatgta gatgtagagt
     2101 tatattatgg aggacaacct ctttattatt acttaatttc agttgaataa tgattataag
     2161 aacctgtatg accaagtagc ttttataaaa ttggttgtac aggatttttg tgtagtaggt
     2221 gatatatttg ttggatttat ataaagatgt tcaatatgtt aagggaatag gacctaaaaa
     2281 agcagataaa ttaaataaac tgggtatatt tacactaaaa gacctattgt attattttcc
     2341 aagacaattt gaagatagaa ataatctaaa gaaaatagca caattagaag atggtgagaa
     2401 agttacgata aaagcagtaa tatcaagtat aaatacattt agcccaaaag aaggtatgac
     2461 tttaacaaag attgatgtaa aagatgagac aggctcggct aaattagtgt tttttaataa
     2521 gagttatata aaaaatactt ttcgtccagg agattcaata ttagtttttg ggaaggttaa
     2581 gaaaaaattt aataacttag aacttacatc atgtgaatta gaatatctta ctaattcacc
     2641 taagaataca tgtagattta tgcctgtgta ccagcttaca tacggtgtta caaataaaga
     2701 aattatgagt ataattagaa cagtacttga agataaagaa ttaattatac aagaatatat
     2761 gccacaaaga attatagaaa aatataggct atgtagtata gattttgcag ttagaaacat
     2821 acattcacca agtagtaagg aatcattaaa aatagcttta tatagaattg tatttgaaga
     2881 attacttata ctacaactag ggttgtttgt atttaaaagt ggcagaaata aagaagatgg
     2941 aataaagttt gaaacaagta aagacttaaa aaaaataata agtgctttac catttaaact
     3001 gactaaagct caaaacaggg cattagatga aattatacaa gatatgaatt tagagaaaat
     3061 aatgaataga ttggtacaag gtgatgtagg gagtggtaaa acagtagtag ctctcttagc
     3121 acttgcaaat tgtgttttaa atggttatca gggcgctttg atggcaccta cagagatatt
     3181 agcagggcaa cattatattt cacttactga gtctctaaaa gattttggaa taaatgtagg
     3241 tctattaata ggtagtctta caaaaaaaca aaaggataca gtattggaac aaataaagaa
     3301 taatgagatt gatatattaa taggtacaca tgcactaatt gaagataagg ttgaatttaa
     3361 taatattggt ttagttataa cagatgaaca acatagattt ggtgttatgc aaaggagcaa
     3421 actttcattg aaaggagcaa atcctgatat attagtaatg acagcaacac caataccaag
     3481 aactttagca cttatactct atggagattt agatatttct attatagatg aacttcctcc
     3541 aggaagacag ccaatagaaa caattgctat tgaaaaaagt aagcgagata gagcatataa
     3601 taaccttgta agaagagagg ttgaatcagg aagacaagtg tacattgtat gtcccttggt
     3661 agaagaaagt gaagccatag aagccaaatc agcagttgaa cttgttgagg agttaagagc
     3721 ggaatatttt cacgacttaa ggcttgggtt gcttcatggt aaaatgaagt caagtgaaaa
     3781 agatgaagta atgagacttt ttaaagataa agaaatagat attttagttt caacaacagt
     3841 aatagaagtt ggtgtcaatg tgccaaatgc aacattaatg ataatagaaa atgcagaaag
     3901 atttggactt gcacaattac atcagcttag agggcgagtt ggaagaggta gtcataaatc
     3961 gtattgtgta ctaatttatg attctaaaac agatgtatgt agacaaagaa tggctataat
     4021 ggaagagacc aatgatggtt ttaagatttc agagaaggat ttagaaataa gaggacctgg
     4081 ggaatttttt gggacaaggc agcatggtct tccagaactt aaggtagcaa acttatttaa
     4141 gcatataaaa atactgaagt tagcacagca agaagctcga tatatattgg gtgaagataa
     4201 taatctacaa ttaaaagaaa acatggcact caaaaaagaa attatagata aatttaaaga
     4261 tacattgaaa gaaatttcat taaattaatt ttaaattgtg ttaaaatatt actaatgtaa
     4321 taatgtgctg aagggagatt gtcatattga gagtaatttc aggaaaagcg agaggattaa
     4381 aactaaatac tccaaaaaat gaagatgtaa ggccaactac agatagagta aaagagtctc
     4441 tatttaatat gataaattca tatataatgg aaagtgaagt tctggattta tttgctggga
     4501 ctggttcttt aggaatagag tgcttatcta ggggtgccaa gtcatgtact tttgtagata
     4561 tcagtaagga aagcatagat attgttaagt ccaatataaa aaaggcaaga gttgaaagtg
     4621 aaagtattat tttaaacctt gattttaaga ctgcaataga taaacttaaa ttacaaaata
     4681 gtaaatttga cataattttt atggaccctc cgtattataa aaatatgttt atagaagcta
     4741 ttgaaaaaat tgataattct aatctattga acgaagatgg aataatagtt gtagaacatg
     4801 atacgaatga tttatttcca gataagatat gtaaattgga gaaaactaga gataagaaat
     4861 atggaaatac tacattaact ttttataaga tggaggcata atatggaaaa taaacctaga
     4921 aaagctatat ttgcaggaag ttttgaccca attactaatg gacatttaga tattatttgc
     4981 agggcttcga agttatttga cgaattacaa ataggagtat taaataatcc taataaaaag
     5041 ggtctattta gctttgatga aagagtaaaa cttatagaaa aaagtacaag ccatttaaat
     5101 aatataaaag ttgtgacttt tgatggttta ttaataaatt actgtcagga aaatgggatt
     5161 ggagcattag ttagaggtgt gagaagtggt gctgatgttg attatgaact tcagatggcg
     5221 catatgaata gagagcttaa tccagatata gagactataa ttttaccaag ttgtacaaaa
     5281 tattcattta taagttcatc tttaataaaa gaggttttgc tctttgatgc tgatataaaa
     5341 aatttggttc caaagattgt tttggaagaa ttaaagaaaa aaactagtgg gggtaattga
     5401 tatatgaaga tagatttaga aatgattgaa ctatttgagc agttagagga gatatttaga
     5461 agtgcttcta caatcccttt ctcacataaa tgcacagtgg ataaagatga ggttttagca
     5521 ataattaatg atataagaac tttaatacca gaagaagtaa cacaggcagt atggattaat
     5581 aaagaaagaa ataaaataat aagtcaagct aagcaagatg ctacaaatat agttgaacaa
     5641 gcgaaaaaag aagctgagag aatacaacaa gaatatcaag ataatataga ggaattaaag
     5701 aaaaattctg aagatgtagt aaaggcatat gtagaatcca gtgaacctgt tgttcaagca
     5761 gaccaaagag caaaagatat agttgataga gctgagagaa tagccaatga gataagaatt
     5821 gggtcaattg aatatgcaga ggatgtgctt tctagtgttg agtataattt aagagaaata
     5881 ttagaagaag taaaaagaga taaagctgaa cttagaccta aaaaataaat tttaaataaa
     5941 taaaaatgga gctatcacac aaaaaattcc aatttatgtg tgatagctct cttttaatat
     6001 agcaattttt tgttatataa ttgtgctttt tagattactt atatcttatt ttgatattat
     6061 aatgtagttt aatcttttat ttgttgaaat caacttattt atttgtataa ataatatagg
     6121 ttattttcaa atttttttaa taattttacc actgtcttct tttagattag agttagactg
     6181 tgcaaaatta taaaataata ctaaatcatg tgcaattatt tggtatggca ctacatgtag
     6241 aaaatataga taataaaatt aaggaggtag tatgaaggaa agggctaaaa aaatattaga
     6301 ttttattatt aaaaataaca atgtaacatc acaagaacta caagaaaagt ttaatgtttc
     6361 aaagagaact atttattacg atattctagc tataaatgaa caactaggaa aaagtggtaa
     6421 tataaaaaat gtaaaacaca aatttatatt tgaaggtaat ttatgtgatg caaggaaaat
     6481 aatttcaact gaagaagata aatttttaga tagtgattac agaaaaactt ttatattaaa
     6541 caagatatta cttggcgaaa aaatatcaat agaaaaacta acaaatgaaa tgttactttc
     6601 aaaaaacaca gtagtacaaa caataactga tgtaaaaaaa taccttcaaa caatggggtt
     6661 aagattagag tataagggaa agtacaaaat tataggtgat gaatacgtta ttagagagct
     6721 gtttctgata attgttcaag aaaatgtact ggaaattaat tctataagtg aggaagtaag
     6781 tagtttcgat acaaaaggac atataaaact tactgattat tctttattaa atctaactaa
     6841 gtttgtggaa tttctaaata aaaggataag agatgggaaa acattatact catataaata
     6901 tctaaatgaa gctaaaaaaa ttagttactt tagtaattgt aaagaacttt tatgtgaaga
     6961 agctaatgaa aatgaacaag cctatatatg cacttacata agttcacttc ccagcttaaa
     7021 tagtgaagtt aaggaagatg ttgttgaaga atatgtagat aaacttatag ataagtttga
     7081 agtaaataca gcaataaaat tagaaagtaa gcatgagttt aagaaaaata ttttaaggca
     7141 cctccactct tcttacaaca gaatacgatt taagtttcct attagaaatc caatgttaga
     7201 tgagactaag tataaacatg agtcacttta taaaattata aaatcaatta tagagaatga
     7261 agaagagttt cctgtatttg aggggataag agaagaagaa ataggattta tagcagcgta
     7321 ttttggagga tatttaagag ggtctaggga taatggtcta agaagaaata aagtcttact
     7381 agtttgtcct aatggtctta tggtatcaaa gagtttggaa atacaactat ataaatacat
     7441 accaaccata gaaatagtag gtatagtatc cattaagcaa ctaaaagaag taaatgtata
     7501 ctacgattac ataattacaa ctatagatat acaaaatgta aacaacgtaa ttgttgtaaa
     7561 tcctctttta acatcatcag atgttcaatt acttatgaac aaactaataa gtgtaaaaga
     7621 aaacgagaag tatttcaatc ttgaattaat aatacaagct ataagaaaaa atggagttat
     7681 caataatgaa gaagcactaa aagctgattt attaaacata atacacaaga tagatgaagg
     7741 agagatgtat caaccaatgt taaaggaatt aattaatgca gaaagggtca atataataaa
     7801 aaatgttaga gattggaaag aagcaataaa aatagcctca aagccattgt tagaagataa
     7861 ctctatagaa gagttatata tagaaaatat gataaagtca gttgagaaat atggaccata
     7921 tattgtttta gctgatagat ttgctctacc acatgcaagt tctaaagaag gagtaaacaa
     7981 gcttgctatg tctttactaa tagtagaaga tgaagttgat ttattaggaa aaccagtaaa
     8041 tatatttatg gtattagctg ctgtagataa cactactcac attagagcat tagcatcact
     8101 atcagagatg atgtacgagg aagaaaatgt aaaactaata attaatggtg ataaaagttc
     8161 aataatagaa cttataaata agcaaaatta gggggaatga aaaatgaaga tattagcagt
     8221 atgtggtttt ggtgtaggtt catcaatggt attaaaaatg acaatccaaa aggctctaaa
     8281 gcagttaggt atagaatgtg aagtagaaaa tacagatata aattcagcta gaggtgctga
     8341 ttgtgactgt atattcacaa gtcaagaatt gttaaatgaa ttaaaaggaa cttgtaatgt
     8401 agatgtatat ccagttaaaa agtacatgga tttagcagaa gtcacagaag ttgttcaaac
     8461 ttttttaagt gataaaaatg ataagaaata gtataagttt taaaaagtaa atttaagtta
     8521 atcagaataa aataaaaact tactaagttc aaaaatagta agaaagagag gagggattta
     8581 gtggtagaaa acttaaacag ttgacctatc actaaaaaat ataatgcttg attaaaaaat
     8641 agctacgcac actcgtgacc ttagtcatga gttagtagct aaaatagtca gcatataggg
     8701 aaacctatat gtagagatag gatagaacct atccaatact acttgaattg ctggaaaccc
     8761 ctaaagctaa ccaaactaaa acataaggat gaaataaacc taagtgtgaa agttacgaaa
     8821 gtagaaaaaa ttggttagat ggtataaggt t
//
LOCUS       DAMJWS010000122         8710 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMJWS010000122 DAMJWS010000000
VERSION     DAMJWS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.127"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..472
                     /locus_tag="PC399_003409"
     CDS             <1..472
                     /locus_tag="PC399_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2594234.1"
                     /translation="QRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLI
                     KENDIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFAS
                     SQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            904..1158
                     /locus_tag="PC399_003410"
     CDS             904..1158
                     /locus_tag="PC399_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594235.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1194..1517
                     /locus_tag="PC399_003411"
     CDS             1194..1517
                     /locus_tag="PC399_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594236.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIF
                     IDYII"
     gene            1791..1943
                     /locus_tag="PC399_003412"
     CDS             1791..1943
                     /locus_tag="PC399_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594237.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2137..3060
                     /locus_tag="PC399_003413"
     CDS             2137..3060
                     /locus_tag="PC399_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2594238.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3327..4007
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3327..3354
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaa"
     gene            4163..4351
                     /locus_tag="PC399_003414"
     CDS             4163..4351
                     /locus_tag="PC399_003414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594239.1"
                     /translation="MNKIATKSRAEYMKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4471..5285
                     /locus_tag="PC399_003415"
                     /pseudo
     CDS             4471..5285
                     /locus_tag="PC399_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5337..5498
                     /locus_tag="PC399_003416"
     CDS             5337..5498
                     /locus_tag="PC399_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594240.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5827..6291)
                     /locus_tag="PC399_003417"
     CDS             complement(5827..6291)
                     /locus_tag="PC399_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594241.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(6427..6744)
                     /locus_tag="PC399_003418"
     CDS             complement(6427..6744)
                     /locus_tag="PC399_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594242.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7027..7323)
                     /locus_tag="PC399_003419"
     CDS             complement(7027..7323)
                     /locus_tag="PC399_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594243.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITNEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(7342..7755)
                     /locus_tag="PC399_003420"
     CDS             complement(7342..7755)
                     /locus_tag="PC399_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594244.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7739..7972)
                     /locus_tag="PC399_003421"
     CDS             complement(7739..7972)
                     /locus_tag="PC399_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594245.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8191..8565
                     /locus_tag="PC399_003422"
     CDS             8191..8565
                     /locus_tag="PC399_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2594246.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
BASE COUNT         3518 a          911 c         1396 g         2885 t
ORIGIN      
        1 acaaagagaa ttatcaagaa aaacaatcgg tagtttaaat agaaataaag caagattaaa
       61 agttgcaaga cttcaagaac atatagctaa tcaaagaaat gattttctac aaaaactatc
      121 tactaaatta ataaaagaga acgatattat ttgcatagaa gatttacaag taaagaatat
      181 gattaggaat cgtaaactat ctcgctcaat ttctgatgtg tcatggtcag agtttattcg
      241 tcaattagaa tacaaggcta attggcatgg tagacaaatt gtaaaggtgg gtaaattctt
      301 tgcaagttca caaatatgca ataaatgtgg gtataaaaac gaggaagtga aggacttaaa
      361 tataagagaa tggatttatc ctagttgtaa tgaaactcac gatagagata taaatgcaag
      421 tataaatata ttaaaagaag gattaagact aataacaatt caaaataaat aacgtatgta
      481 agaaccgtag gaactacggg gatagcctgt ggagaattag taagacatat gttagtatgc
      541 aaaattctgt gaagcaggaa acctgtgact ttagtcatgg gaggttcagg ctagtattac
      601 tcattattgt attcaaaagc taaagtggaa acctagtgaa tatatgaatt tagaagttaa
      661 tgagagagca ttagcagccg actcaatact tattaaaatt gaagatgaag aagaaacaat
      721 gaaagaagct gaaagagaga aaaagagagg tagaagaagg tagaatttaa agttaaaata
      781 gttacattgc taatatatga aaatgttaca aatttggaaa tagatggata tatatgttgt
      841 atagtataat gtagataata tatcaccaag taaaaaacaa caaataaatc taaattatga
      901 ggggtggaaa ataaagatat taaacttaca atatgtatat ttgcttttgt aattggaata
      961 atagtgttat tttgctgtat aggtctaggt caatttaata tggattgtat tataaaacaa
     1021 aatggtgggt caatagctac cgaacaatac aacatatatt tagaacaatc tatttcacaa
     1081 tatagaaatt ttggttctat actggtttta ctaggaggaa taggaatact gtttgataaa
     1141 agtgtcagaa aatactaaaa aagtatattt ttatttagga tgtgatgaat gatatggcaa
     1201 gagatgtatt tagtgtaaag atgacaccac tagaagcaca gaaaatggta gaaaaaaatt
     1261 tggaagcaga tttagtttct gttgatattt ataacctaga aaataataag tctataatag
     1321 ttacaatctt tgaaaaatat tatgcaagaa gcaatagtga tgcaggtttg gtagtgattt
     1381 gtgaaaatac tacaggaaaa acacttgtta aaattacctc aactggtagt gcaattggac
     1441 tatttcagat tgattgggga acaggtaata atttgattaa aagagtaaaa aaaatattta
     1501 ttgattatat tatttaatta ttttatgtag atattcttag gttggtatgg aatttgtact
     1561 aacctattaa gtaatcaata aaactaaata aacatagaaa gcacttacaa atatgtaggt
     1621 gctttttatt gaaaaaatag aaaataaaat aaaaagtaag atataggaaa aatatgtaag
     1681 aattatatgt tataataatt gtagcaagaa gatgtaatct acaatttata gagtggagtt
     1741 catgtattaa aaaattatcc tcccaacgat aagaagggag gtgaatatac atggataatt
     1801 ttttatttaa tgtgttagct agtgtaacag ctagttacat agtttatttg attagcaagt
     1861 taatcaaaaa agtaaaaagc cactctaccg caaagagtga cttaaagatt gatataaatt
     1921 ttaaatatca tcgtaaaaaa taactttcag ttatagaact tcactctacc gcaaatagat
     1981 tgtagttctt cttgttttta ttataccaca aattagaaaa aatattgcat ataatatttt
     2041 ttatagtcaa taaaaatatg aaatttttta tacacaatga tgatgaatct aactagatga
     2101 ataaatttaa ttgtaaataa gaaagtactt acttaaatgg taggtgcttt tgttttgcca
     2161 aattatgcta taatattgag tgagatgagt aatttgtggg ggaattttat aatgtggata
     2221 ttagtaccaa taataacaat agtattactt ataatagctg tttcttctat gcaatatatt
     2281 ttagttatga tagctttttt gttgattata tattctttta tagaaaagaa aatagttatg
     2341 ggtcttgtgt cagttctttt tttcacttat tcaatttatc tttgtgcaac ttgggaagat
     2401 aaatccttga tagcagataa taaagttgag actgttaagg cacaaagaga aactgtagaa
     2461 agagaaaaag aaatggaaag aagaagaata caagaagaag tagacaaaga aagatatatt
     2521 gaaaaacatg gaatggaaat atcagaaaat gatttaaaag ttaagctaga ggctttagta
     2581 cctcaagaat ataaggggaa gaaatatgaa ttaaaagtag gaaaatttaa aagatatagt
     2641 atgtattttg atttaactgt acaaaatgaa aaatttagta attctgaaga atgtaaaaaa
     2701 tttgtaaaag aaattgctaa tgatcttaaa aaaattaaaa taagcaaggc gtactttaag
     2761 tttcattcaa aagatgacgg cggtatatat aattctgttt atattgatta ttttagaaac
     2821 atacaaaata atgtagataa tgttgaaaat ttagaattca atgagtttga acttaaaacc
     2881 gaagaagaag agaagcgaga acaagagaaa atagaacaag aaaaaaacag ttataataac
     2941 tacattcaaa acagagtggt tgacccacta gatagaataa agaaactaaa agagttgcta
     3001 gattcaggag caattacaca ggaagaatat aataaaaaga aaaaagaatt attagaatag
     3061 ataatatagt aagcacttac aagtatgtag gtgcttttaa atttacaaag tattccattt
     3121 taattttata gtttagattt tatgatataa taaaaatata gaagttttgc agtgtgcgat
     3181 atttgttaca aagtagggct taatacttga aatctaagat gttgagggtg cgtgataagt
     3241 gttatcaatt gcactattgc ccgctcactg caattttaag agtattgtat atatgtaggt
     3301 attggaaatg ctaagtttat tttggggttt tagattaact atatggaatg taaaactgac
     3361 tcgggagcat ataataagta atatcaagaa tagttttaga ttaactatat ggaatgtaaa
     3421 attcgtaaaa taacaattgt caggaatatg agaagtttgt tttagattaa ctatatggaa
     3481 tgtaaaattg gactcaaaat ataagttaaa aaaattttta agttttagat taactatatg
     3541 gaatgtaaaa atttttgacg ctatgaagaa atttactaca aaatttgttt tagattaact
     3601 atatggaatg taaaaacttt tttcatcctc ccaaacctct tgttcgctgt cgttttagat
     3661 taactatatg gaatgtaaaa aagaaaataa gtttaaaaaa aggtttcatt attatatcgt
     3721 tttagattaa ctatatggaa tgtaaaacca attatgtaca gtttagttac tgtgttaagt
     3781 cttgttttag attaactata tggaatgtaa aatcctttta taaatatctt ctgacatgtg
     3841 tatatgcttg ttttagatta actatatgga atgtaaatcg gctacaacgg tataaattaa
     3901 atttagcagg gtacatttta gattaactat atggaatgta aattgctgtg atttcaataa
     3961 taaaacaatt aataattgtg ttttagatta actatgtgga ttcaaaatta aataaacaaa
     4021 gaaagcactt acaaatatgt aggtgctttt attctgctca aaattggtcg gttgggtaaa
     4081 ataattagaa aaagttagta aaaacctatt gactgtaact cgttacaata ttgttattaa
     4141 tgtaacgagt tacagaaaag aggtgaataa aatagcaact aaaagtaggg cagagtatat
     4201 gaaaaatcgt cgaaaagata aaagaggttt tagtgtactt ttagacaaag aaaagttaga
     4261 taaatttgat gaagtgttag aggaaaagaa tctaaccaag aaagaatggc tagaagaaaa
     4321 aatcgacgag gaactggaac aaaaggaata aaaaataagg gtcactccca ccgaccaaag
     4381 tttgagtaac ccctatgacg tatactatcg tatatcaatt atagtatatg tcattcctta
     4441 aaaaaatcaa ttattaagga gtgtaatatt atgaaaaatg aattaatgat gtttgaagga
     4501 aaagagattg aagtatttga atttgaagga agaattttat tcaactcaaa agatgtagta
     4561 aactgtttag acataaaaaa tgttaatgag aatataagac ttatgaatga aaagcaagtt
     4621 gtaaagttaa gaaattcgga tatcagtaat actgacatcc gaaaacttaa taatgctgga
     4681 gaaaattttc ttactgaaag tggagtgtat aaattaatct ttaaatcaag aaaagaagaa
     4741 gctgagagat tccaaggctg gataagtgat gaagttttac caagcataag acaaactggt
     4801 gcatacataa ataataatgc aataccgaaa agttgagaga aaaagcaagt gagattgaaa
     4861 aattacagtt ggtttataac agtacatcta tgttaaaaga attgctagat gatgcaggct
     4921 ttgacaataa atcaaaacta ttaacagcta aaacattata taagaaagca ggaattgatt
     4981 taccaataga gataaacgaa gaagaacatt attttgatac aaaacaaata gcatctaaac
     5041 tgaaaatata ttctaagagt aataaaccag ctcagatggc agtttgtgag attattaaaa
     5101 agattgattt agaagatggt gaagttaaag gtgtttggga aattaatggt tcttggactg
     5161 gtactgtaaa taaatataca aagagtgtaa tagataaggt gagaacttgg atagaggaaa
     5221 ataatagacc taccaagatt gcaggtgaga agaagaatta ttatgtattt tacaaaattg
     5281 agtaaattta tcagttgtat taaataattt agtttagttt tggggggatt aatacaatgt
     5341 gtgagaattt acttgataat atgtatattg aaaagagaaa agaagaatat agaattaaac
     5401 ttttgaaaat aagagaaaca gatatagata tatataataa gttagaaagt atagtatata
     5461 aactttctga gaaaaaatta gagaaaaata ataaatagac atataaagca cttggatatt
     5521 ctactgtttc aagtgctttg tttggtataa aatggtataa tggagataga gaattatatt
     5581 aacccaaaaa tggagaaaat agagaagatg atttagttga cgctttatca gtaggagtac
     5641 ttgcaggtaa gacagaatct ctagtactat tagttggaaa tggaataagt gatactcaaa
     5701 aaagtttaat aaataaaaga gaatttagaa atataactca aattggtgga aatggaaatg
     5761 agaaagcatt tagtgaagta gaaaacttag ttaaatagat tagaaggtag caactagagt
     5821 tggttgttac cttctttttt tatactttct ctctctaagt attttctttt tcatatcttc
     5881 taatacatct aatgcagtag tataaaattg tatattattt ctataaggta tagctattgg
     5941 aagaatgccc ttgttaacta aaaaaataag aagtacaata aataaaagtg tcataatcat
     6001 tctaataaaa taattagtga tatctgataa atcaaatgtt aagttaattg ttgtagaaaa
     6061 tacaacagcc ataatagata tatacaaagc aaaaatgggg ttactatttt tttgtaactg
     6121 tcctttaagc ctagattgct ctatttctat atcttgaata ctttttatgg aatccatttc
     6181 actataatac ttataaactt ttaaataata ttttttatac ttgtttaaag cttcttcatt
     6241 atctttaagg tctttgtcac ttaactctga tttaacttcc ccaaaatcca taaaatccct
     6301 cctacaatgt tatataatat actttaattt atagcatatt taagaactta tatcagcaat
     6361 ttgtcgaacg attttagcta aaaatagagt ataatgatag gcttattatt tataagatat
     6421 tattttttat atagttatat atttctttct aattagataa tataatgctg ttgttataat
     6481 ggctactaat attattgcta ttattgtaat atctaatctt cttgtaaata agcctattgc
     6541 tatatactca agacctgtta catagcgata aagtacttct agtttaacag tttttttctt
     6601 atctatattt ccatatattg attctaattt attaaaatat tttttactac aaaaagcagt
     6661 tttaggttta attaaatata ttattgtagt tcctattata actattattc caataagtat
     6721 aagattgata tttccaaaat ccatagcaga tacctcctat taaatttgct tttcttaatc
     6781 aaagtatatc agtaacacaa gtacaaagag catctaaaat attacaaata ttaacaggat
     6841 agataaaact atagtaaaaa ttgtaataat aaagcgaacg aaaattatca tactaaaaca
     6901 taagacatgt tataattgta ttagatagaa gattaacata caccaaagca ctccttacaa
     6961 taaagagtgc ttattttttt gaaattcatc aatatataaa ctatcaagaa ccttattcaa
     7021 cacaccttaa aatcgattta aatagctctt tttgataaca taatttatat gatataataa
     7081 aaaaatagaa gtgtaccacc aatacacttc tatagctata actaagcatt ctcttgctgg
     7141 gggagtgctt ttttcatttc tccaaacaac attatttact tcattagtaa ttatcaaatc
     7201 aatctcatta tctttcaaaa tctctgttgc agtacaaatt agattatcta agtcaaaact
     7261 tcgagttatc tctagttgtt tgttatttac ttctcttttt agtatataag ttttaaaata
     7321 catttatatc accccttgta tttactcttt tttctctaaa aatatattga taagtaaata
     7381 tgcaacaaat aaaaaaatct caatgacaca tagtgttata gctataacta gaagtgattg
     7441 atttttccat gcactataag ctatgatgaa aagaaatgta aaagtaaata atgtataatt
     7501 cataatttta actaagatat gacttgtttt cattgttaag tacaagtctc tttcatctgc
     7561 actatcgtgt aattcttcaa caatgccttt tcttgaaaaa gcattacaaa aattaaagat
     7621 tgctattaca attaaaagaa atgatgaaat taggaatctt ttatctttta aaatgcaata
     7681 aataactaaa ctagcacaac ataacataca aactataata cccactataa aacttcgttt
     7741 actctttact ttcatgtttt ttatcctcca tttctttatt ttcttttaaa caacatagtt
     7801 cttcaatcgt tgtaccaaac acttgtgcca tacgataggc aagcataaga gaagggctat
     7861 attgctcttt ttcaatagaa ataattgttc tgtttgatac atggactaaa tcagcgagtt
     7921 gttgctgtgt tagacccata tttgttcgaa attctcgtac ttttgttttc attccataac
     7981 ctctttctat atgaagctaa cttcatgtga agttagcttc ataatatcct aaaatgagta
     8041 aaatgtcaat ctcattagat aagtattata aaacctaaaa gaataatatg gtaaaaattg
     8101 gatggaatta tttaccttaa ttctcttgtt tgtaaaactt tattgatata gaatttaaca
     8161 tatacataaa tcggcaaagg ggcgcgaatt atgaaaatta gtaaattacc agaagcggaa
     8221 ttaaaagtta tgagatatat ttgtgaagct aacaaggtat taacttctag ggaattagtt
     8281 gctgcaatgg aagaaaaata tgagtggaat gaatcaacta catttacagt tttgaaaaga
     8341 ttagagcgaa gggagttttt aagtacagag aaaataggca aacttacaca ttataatata
     8401 ctagtaaaag agaaaaaata tctaaggttt gaaacaaagg aatttttgaa gaacatacat
     8461 aaaaattcaa tttcaagttt aatgtcagcg ttacatggtg aagacgacga agtggatgaa
     8521 gataaattaa gtgaactcga agaatatttt aagaatttga aataagaaaa tgacaaattg
     8581 atacataata aataaaatga aggtatgaaa ttataacttt ttctaccttc attttgttta
     8641 ttattctgaa catgttaaga aaatgaaata agtttgtatt ttagttatat aaattttaat
     8701 agaatatgta
//
LOCUS       DAMJWS010000123         8634 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMJWS010000123 DAMJWS010000000
VERSION     DAMJWS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.128"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            301..1287
                     /locus_tag="PC399_003423"
     CDS             301..1287
                     /locus_tag="PC399_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2594247.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1456..1604
                     /locus_tag="PC399_003424"
                     /pseudo
     CDS             1456..1604
                     /locus_tag="PC399_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1623..3104
                     /locus_tag="PC399_003425"
     CDS             1623..3104
                     /locus_tag="PC399_003425"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF2594248.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3130..4101
                     /locus_tag="PC399_003426"
     CDS             3130..4101
                     /locus_tag="PC399_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF2594249.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4357..4959)
                     /locus_tag="PC399_003427"
     CDS             complement(4357..4959)
                     /locus_tag="PC399_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2594250.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            5240..6451
                     /locus_tag="PC399_003428"
     CDS             5240..6451
                     /locus_tag="PC399_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF2594251.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6519..6716
                     /locus_tag="PC399_003429"
     CDS             6519..6716
                     /locus_tag="PC399_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF2594252.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6750..7721
                     /locus_tag="PC399_003430"
     CDS             6750..7721
                     /locus_tag="PC399_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2594253.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            7908..8285
                     /locus_tag="PC399_003431"
     CDS             7908..8285
                     /locus_tag="PC399_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2594254.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3508 a          896 c         1377 g         2853 t
ORIGIN      
        1 ttttttaagt gataattaag tgtttatcga taaggctatg gttgtaaaaa tcatagcctt
       61 atttttatat tataaataaa ttagagtatt tatcaattca tagtagataa aattgaaatt
      121 aaatataaca acaaaaaatg aaaattacaa ttaatttaat gttaaagact caattatttg
      181 gatgtggaat ggatagtaca agtagtatat aaaaagtatt gactggacaa ataactatat
      241 tttattttaa atatataata taatgtaata aaatatatca aatgtagaag gtgataacct
      301 atgaaaaaaa taacaataaa tgatattgct aacttggcag gagtatctaa aagtactgta
      361 tcaagatatc taaacaataa agatatcagt gattctacta aagagaaaat aaagacaata
      421 atagatgaat atggatatga gccgaatgca tttgctcaaa gtttgagagc taaaaaaaca
      481 tattttattg gtattataac accgtgttta gattcttttg taaaatctaa aattatgatg
      541 gcaatagatg aagaactcaa aaaattaaaa tatacatcac taattattaa tactaataga
      601 aaaataaggt cagaaataga tagtatatca aagttagcta gtctaaaagt tgatggaata
      661 atactaattg gtaccgaaat aactaaagaa cataagaatg aaattgaaaa gttagatata
      721 ccgatagttg tagttggaca aaaagttgat ggtatcaata gcatagtgaa tgatgattat
      781 ggagcaggct ataaaatggg tcaatatatt gctaataaag gttacaaaaa cattgtatat
      841 ttaggtgttg atgaaagtga tatatcagtt ggtttaaata gaaaaaatgg agtacttaat
      901 gggttaaaag ataaagggta tgatgcaaaa gtattttata ctgactttga ccaagaaatg
      961 tctatacaaa gaagtggaga aatgctagaa agtgaaaatc cagatataat aatatgtgca
     1021 actgataata tagcaatagc aacaatgaaa gaaatcaata agagaggtaa aagtatacca
     1081 caagatatat ctgtagctgg atttggggga tatgatgtat tgtctattat aagccctaaa
     1141 cttacaacaa taaagtttga aaataaaaat gcaggtaaag tagcagcaaa tacgatagta
     1201 aatttaatac aagaaagaaa agaaccacta ttaaaggaga ttaaatttga attaatagaa
     1261 ggtgaaagta caataaataa aaattaagat acacattaca tatattagta atgtgtattt
     1321 ttttactaaa aaaactataa ttctaaaaat taggaaaact tttttgaaag aaatattgca
     1381 tctgaaataa atttatgcta atctttaatt aaaccggttt cgaaaccggt ttaattaaac
     1441 cgatagaata gcaaaatgag tgcaaaaata actgattgta acaaagtatc aaagtaatta
     1501 ttggagtaca taggaataaa caaaatataa aagatgtagc acattgtgta ataagactaa
     1561 gaattgtttt agatagcaat aataaagcaa atataaaatt ataaaagaaa aggaataaaa
     1621 atatgtacaa agagcctaaa tatagaacca tattagaatc gacaaaagaa gaattaaata
     1681 aattaagaaa tatatcttta aatgataaat ataaaccact atttcatata catccacaac
     1741 atggactact taatgaccca aatggattag catattataa tggtaagtac catgtatttt
     1801 atcaatggta tccatatgat gcaactcatg gaatgaagca ttgggcttat gtaagttctg
     1861 atgattttgt aaactggaat agagaagatg tagcattaat accaatcgaa agttatgaat
     1921 ctcatggagc atattcaggt aatgcgatag aggtagatgg aaaattgcat atgtattata
     1981 cagggaatat aaaatatagt gcagaagata gatatgcata tcaaaactta gctataatga
     2041 acaaggatgg taaaataact aaatatgaaa ataatccaat agtaagtgaa ataccaaaag
     2101 gatatacagg acatgtaaga gacccaaaag tatttaaaag aaaggataaa tactttatgt
     2161 tacttggagc acaaacaagt gataaaaaag gagttataat agtatatgaa tctaaaaact
     2221 ctatagattg gaattttaaa ggagaattaa atgtaaaaaa catagatgaa gattttggtt
     2281 acatgtggga atgtccagat tatattaata tagatgaaaa agatatatta atattttcac
     2341 cacaaggagt agaacctaaa ggatttgatt atcaaaatat atataatgta gtatatgcta
     2401 ttggtaatat ggatttagat aatttaacat ttgaaataga tacaatgaaa gaactggaaa
     2461 aaggatttga tttctatgct ccacaaacat ttataaagga ctcacaaata atactatttg
     2521 catgggctgg aatgggagaa gttttatacc ctacagataa aaacaaatgg gcacattgtc
     2581 taactgttcc aagaaaatta aatataaaaa ataataagct attgcaaatg ccagtagatg
     2641 aattaattaa attaagatat gatgaaacaa gtgggcaaaa tactataaag aataatatta
     2701 atattataga aaatgatgaa aatgtatatg agcttaatat taatataaaa aatattgaca
     2761 gcaataaatt tgggctggaa ttattttcat cacaagatga aggtgtaaaa ttagaattta
     2821 acaaattagg aaacattgta actttagaca gaagcaactt taaaaaagtt ttcggtgttg
     2881 aatatggaac aaatagaaaa gaatatataa atattgatga aaatactaat atcaaagtac
     2941 tagcggatag aagtatttta gaaattttta taaatgaagg agaagtagta tttactagta
     3001 gaatatttgc taaagaaaat tctaatcaaa taagggttta tagtgataaa atagtagagt
     3061 atgagtatac taaatttaaa ttaaagcaag gtattgaatt ataaaaacac attataaggg
     3121 gtagaaaaga tgaaaaaagt aattagtata ggagaagctt taatagactt tatacctaat
     3181 caaaatggtg gtaaattaaa aaatgtatca gaatttagaa gagtagcagg aggagctcca
     3241 gcaaatgtta gtgctgttgt agcaaagctt ggaggaaaat cgagttttat atctaagtta
     3301 ggaaaagatg catttggtga ttatataata gatgtattaa atgaagtaaa tgtaaatact
     3361 gattatgtat tgagaacaag taaagcaaat acaggacttg cttttgtatc attaaaagaa
     3421 gatggtaatc gtgatttctc attttataga aatccaagtg cagatatgct tttagaagct
     3481 gatgaagtta aaaaagagtg gtttaataat tgtcatatcc tacatttttg ttcagtagat
     3541 ttaatagata gtccaatgaa attagcacat aaaaaagcaa tagaatacgc acttgaaagt
     3601 aatagtataa taagctttga ccctaatata agattacctt tatgggatag tgagcaatct
     3661 tgtaaaaagg caatatcaga atttttacct tttgctcata ttgttaagat atctgatgaa
     3721 gaattagaat ttataacagg agaaaatgat atagaaaaat cattacataa actctttgtg
     3781 gggaatgttg aactagtatt atatacaaaa ggaaaagatg gagtagatgc atacactaaa
     3841 aaagtaaaag gtatgtgtaa aggggttaaa gtaaatgcaa tagatacaac cggagcagga
     3901 gattcttata taggttcatt tttatacacc ttattgtata aacaaataac attgaatgat
     3961 ataaaagaaa tgaaacaaga aacgttaaat gagtatttag aattttctaa ttattatgca
     4021 gctcgtagta caactattaa aggtgcgata agttcatatg caacaaagga agaaataact
     4081 gaatttataa aaaatttata gtaggaatga gtaataggtt ttgaaggaag taacatgtaa
     4141 agagttttta aggatgttta ctatgaaaat aattaacata taaaaagcaa taaggaggct
     4201 gtctcaaaat agagataagc ctctttatca ttgttgaaat gataaaaaat attttaatta
     4261 ttgttacttt ttagaagaat atatatctta ttttaaataa gcaaattatg taagatttat
     4321 ttatattatc aatactttac ttggagtatt atgtctttag cagttatcta attcaattga
     4381 attagataaa atataataaa tttgttttgc aaattgacga gaatgttctt ctttttcttt
     4441 attcataaaa ttaggtttac ctaaatcacg ttcaacaagc attcctatat attttaactg
     4501 tgaatgctta cagtagcgct tcataccatt ttcccaaata tcagcaccct tttcaggtct
     4561 atatccacat gttgtaataa gtgctactga ttttcctgcc catagagcgg ggcctttctc
     4621 attaccatag tatttattca ttccatacac caatctgtca agtagtgctt tcattggaga
     4681 agtgcagtac caagaataaa ttggagtagc aaaaacaatt aaatcacaca taagaatttt
     4741 cttgaaaatt ttctgaacat catctttaca ataacatcca aaatcaaata agttatcctg
     4801 acacttttta caagcaatac atggctctat tttacaatca tataaccata ttacttcaat
     4861 atttgaatta taattttcta attcctcaat aaaaggtttt aaaatagcag cagtattacc
     4921 ttttttctta ggacttccca taagaatgca tacttccata ttgatacctc cacagtattt
     4981 taaaatagtt tttagtgctt aatatagttt taggatacta tgagaatacg ttaacaacaa
     5041 tacactttgt atatttgtaa taaaattgaa acaaattgtt ttatcaataa aagttggaat
     5101 gtatctataa ttaaaaatat tttaggtatg tttttacata tatgtgtgta gttaactatg
     5161 gtaatttatt gtaaaattat cttataggat tataattgct taatttatga ttatatttaa
     5221 aatcttggag gtaaaaaata tgtactcaaa tgaagactat aaatttatag aagaaaatgc
     5281 accatgttat atatatgatg agactaatat aattgaaaaa tgtaaggaat tgaaaaataa
     5341 tattttaggt tttgattttt tatattctat aaaaacaaat ccatataaca aaattgtaaa
     5401 aagcattgca gagcaaggtt ttggagcaga tgcttcttca actaatgaag ttatgatttc
     5461 atcaaaatgt ggagtttcta aagatgatat ttattattca tcaccaggca aaacagagat
     5521 tgatatagaa actgttttag ataaatgcat aattattgca gatagtatta cagaaataga
     5581 acttataaat aagatatcta aaaagcataa tcagaaagtt aaaataggaa taagaataaa
     5641 tcctgatttt actatggatg atgatagtgc taaatcaagc aaatttggta tagatataga
     5701 agatatgagc agtgttttta agatgttaag tatgtgtgat tatgtatctg tagttgggat
     5761 tcatatacat attaaatctc aggtattgga ttttgaaaag ttaggacatt attatgaaaa
     5821 atgttttttt atagctttaa atttaaataa aagtaagtat attaatattg aatttataaa
     5881 ttttggtagt ggacttggag tagcttatga taatcttaga gaaaatccac ttaatcttaa
     5941 agagctttca aaattaactc aaaagattgc ttttactaat tcaaacacac taaaatcaag
     6001 acttcttatt gaatcaggac gtttcatagt ttgtaaatca ggtaattatt atgttaaaat
     6061 tgtagatatt aaagtatcaa gagggaaaaa atatcttatt gtaaaaaatg gacttaatgg
     6121 atttatgaga cctattattt ctaatttaat tgaaaatatt acttgtggag tagaattaga
     6181 aggtcaagag ccattatata catcaaatca tgccttttct ctttcagtat taaataattt
     6241 aaaagaagaa gagtatgtaa atgttgttgg gaatctctgt acatcattag atgtaattgc
     6301 aaataatgtt aaattgaaaa aagcaaacat aggagatttg atttcaatta gtaatgctgg
     6361 aagttactcc tattctttat caccgctatt attttcaagt tatgaatttc ctaaacaata
     6421 ttataaaaga aaagatggat atattgagta aacaatagca taaattaata aacttagtat
     6481 tattgaaaaa atttaaaatg cagggggtgt aattctatat ggaattgtgg aaaagaaatt
     6541 tatttgtatg ttggataggt atgtttttta gttctatagg gatgagccaa atagcaccta
     6601 tacttccatt atatattaaa cagttggggg taactgatgt tagtttaata caacagtatt
     6661 caggtataat ttttggatgt acctttgtag tagcagcatt cttttcgcca atttgaggga
     6721 aagctgctga taaatatgga agaaaatcaa tgcttttacg tgcaagtatt gggatgggaa
     6781 tagttatatt taccatggcg tttgttcaaa atgtttatca gttattagga ttaagaatac
     6841 ttcaaggagt ttttacgggt tatgctactg catgtactac tcttattgca acacagacag
     6901 ataaaaatca ttcaggatgg gctttgggta ctcttgcaac tgcatcaact actggctcat
     6961 taattggtcc aacagtagga ggatatatag aatcaatctt aggtcttaaa agtacattta
     7021 ttataacagg aggattgttg tttgtatcat ttatcatctc tattttattt gtagtagata
     7081 attttaaacc taaagaaata aaagaatcaa tttctatacc taaagaacaa ttaaatatat
     7141 tgccaaataa atttttaatt gcatctattt ttgtaaccac attcattact caactagctt
     7201 tatactctat agaaccaatt gtaaccatat atatatcaca acttacaaat tttgcatcaa
     7261 atgtagcttt aatagctgga ttgacatttt ctgcatcagg attagcaaat ttattagcag
     7321 ctcaaaaact tggaaaaatg tcggataaaa taggtcctca aaaagtattg ttaatttcac
     7381 ttttatgggc tgggattata tttataccac aagcttttgt aaaaactgca tggcaactga
     7441 tgttgttgag atttttatta gggttgtcag ttgcagggct taatccttct gttaacagct
     7501 tgcttaaaaa aattgcacca gaagaatatg tgggaaaaat atttggatat aatgcatctg
     7561 cccagtatat aggctgttca tcaggagcat ttttaggggg acaaatatca gcacatttag
     7621 gtattagaac agtatttttt tcaacaagtt tattgttgtt taggaatgca ctttggatgt
     7681 ataaatttac tggattgttt attaaagata agactaaata atcatactat ttacatttct
     7741 aattttatga tgtaaaataa atatttttta tgtaatttat catagaatta tataaaaaat
     7801 aaccctatgg taaaaattgg aaaatattat tatcgacaaa atctttgact taaaaactat
     7861 attgcgatag tattaattta ataaaagttt gagaggggcg taaggttatg acaatttcaa
     7921 aaataccaca agcagaatta aaagttatga agtttatttg ggaaaaaaat gatacagtaa
     7981 catcaaaaga aactattgaa gctatggaac gaaaatatgg ttggaaacaa acaactacat
     8041 tgacactttt atcaagactt gttaaaaaac attttttaag tgcagaaaaa atagatagat
     8101 acactcatta tacaatagtt gttggacata aggagtattt aagtgttgag acaaaagatt
     8161 ttttaagcaa tatacacgac aattctttac agagtttaat ttcagcacta catgatgatg
     8221 aagttgttga taaagatatg ttggatttat ttgaaagtca ttttaaaaat ttagaagaag
     8281 agtaattata tcaggataga actctaagaa tttaattaat ctatggctga aaaccataaa
     8341 tggtattatt taatcagcta ctcactattc ataaagtaat attaaatagg ttctctaatt
     8401 agtaaaatgc ctaaaataat tggaataggg agtatcaagt taaatgatac cacttattta
     8461 aataagaaat gatttttaaa atttaaaata attttataac ttgtcaatat tcattaatct
     8521 aaagtattat ttctatttaa ccatatcaaa atatatttta ctatttttaa ttattacaat
     8581 ataaacctgt cttttgttat tataggtaaa ctaaaagtaa gaaatgattt ttat
//
LOCUS       DAMJWS010000124         7865 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMJWS010000124 DAMJWS010000000
VERSION     DAMJWS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.129"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(567..755)
                     /locus_tag="PC399_003432"
     CDS             complement(567..755)
                     /locus_tag="PC399_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594255.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            1015..1158
                     /locus_tag="PC399_003433"
     CDS             1015..1158
                     /locus_tag="PC399_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594256.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNK
                     KRH"
     gene            1665..2042
                     /locus_tag="PC399_003434"
     CDS             1665..2042
                     /locus_tag="PC399_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2594257.1"
                     /translation="MKEEILVEKLLRAELIVMEYLWKKDSLVTKKEIVKEMRQVYGWH
                     KSTIKILLKRLVDKGYLARDIIKFQSHYKIIIDNKEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKSISKEKLDALEEYYRNLEE"
     gene            2152..2535
                     /locus_tag="PC399_003435"
     CDS             2152..2535
                     /locus_tag="PC399_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2594258.1"
                     /translation="MKISKLPEAELKVMRYIWEANDVLTSKELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNAMVKEKKYLRFETKEFLKNIHKNSISSLISALH
                     SKDDEVDEDKLIELEEHFKNLKEKE"
     gene            2720..3304
                     /locus_tag="PC399_003436"
                     /pseudo
     CDS             2720..3304
                     /locus_tag="PC399_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            3330..3743
                     /locus_tag="PC399_003437"
     CDS             3330..3743
                     /locus_tag="PC399_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2594259.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            3747..4490
                     /locus_tag="PC399_003438"
     CDS             3747..4490
                     /locus_tag="PC399_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF2594260.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            4490..4960
                     /locus_tag="PC399_003439"
     CDS             4490..4960
                     /locus_tag="PC399_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2594261.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            4977..5735
                     /locus_tag="PC399_003440"
     CDS             4977..5735
                     /locus_tag="PC399_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2594262.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            5719..6561
                     /locus_tag="PC399_003441"
     CDS             5719..6561
                     /locus_tag="PC399_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2594263.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(6721..7626)
                     /locus_tag="PC399_003442"
     CDS             complement(6721..7626)
                     /locus_tag="PC399_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2594264.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT         3067 a          879 c         1272 g         2647 t
ORIGIN      
        1 ccatataaaa actgtttttt gtcgaatgta tgtttgaaaa aataaaaatt agtaaattat
       61 cttaaaaata attagatata ctttggaagc aataagaata ttaacaataa gataaatcgt
      121 tgaaatacca acatgtgtta aataataaca ataaacaaat aataaaattt tagatttcag
      181 aatatgaaag aaccaaaaaa cgattaacta gtaatcttaa ttaaattcat ttagttgatg
      241 ttaaagtatg aaaactatag tagaagttgt aataataaaa cgaacgaaaa ttatcatact
      301 aaaacataat acatgctata attgtattag ataaatgctt gaatatatac caaaagtact
      361 ctttttataa gagtgcttat ttttttgaaa ttcatcaaca tataaactat caagaacatt
      421 actcaacaca ccttaaaatt gatttaaatt ctttttcata cacaaagtta tatgatataa
      481 taaaaaagta aatatgtaac cccaacacat ctttactaag tcaaacatta ttatatagag
      541 cattcttcat tatggagagt gctttttcat ttctttgaat aatcatgttg attatttata
      601 atattttcat ttaatttatc tttatctatc aaatttaatg cacaattaat acaaatgtta
      661 gttttcatat gacttttctt atgaaaagta atatacttat tatcatcttt atttattcct
      721 ttattacagt aatcacataa tatagtctta gtcatatttt tattcctttc attttatatt
      781 ttctataatt aagtttaaca tatttagtat atacctatgt atatatatat ttaaaacaat
      841 gttttttatt gcaatataga atatattgta taaataaaaa atatgaagaa tagatatttg
      901 ataattgtaa aattatgtta taattaaaat gcaagaataa tcttgtggaa ctacaatcta
      961 agagtggagc ttcattttct acattccatc ccttaaaagg aaggaggtgg agaaatgagc
     1021 gaatttttac taggagtgtt agctagttta acagctagct ttattacata tattatttcc
     1081 agaaaagtaa aaagccactc tggcaggagt gactttgagc ttgatgtaaa aatcaagttt
     1141 aataaaaaac gacattaata tttagttaat gaaacttcac tctaggctaa tagattgtag
     1201 tttctttttt tgtttttgat acgaatttac atctttatta tatcgcattt tgagaaaaaa
     1261 taaaactatg aatattagaa atatatttta ttttatctct aagtatatat attattagcc
     1321 acctaaaaaa ctttcacaaa aacaaaaaga gtatatgttt ttgataactg gatgatataa
     1381 cttataactc attttacaat tcttagttca tatatattta tgccactaaa tttctctagt
     1441 tttgccgatt tagttttatt ctacaactaa aataatacaa tagaattagt tattattcaa
     1501 caggatagtt tgaaagttga ataattttat agatatgaaa gttatttttc ttctaagaaa
     1561 acaaactaaa acagtagaat atttggtaaa aattacctat agtttttgtc ttaaaaagct
     1621 cttattatta acttgaacta gaacaatcga aggagtgaca taagttgaaa gaagaaatat
     1681 tagtagaaaa attattaaga gcagaattaa tagtaatgga gtatttatgg aaaaaagatt
     1741 ctctagtgac taaaaaagaa attgtaaaag aaatgagaca ggtttatgga tggcataaaa
     1801 gtacaataaa aatactacta aaaaggctag ttgacaaagg ttatttggcc agagatatta
     1861 taaagtttca atctcattat aaaataataa tagataacaa agaatattat gcttttaaga
     1921 aaaaagtgtt aaaatctagc aaaagcagaa aaataatgcg ttctcttact actacacata
     1981 agtctataag taaagaaaaa ctagacgcat tagaagaata ttatagaaat ttagaagaat
     2041 aatttcgaga tggaaaaaat tggatggtaa tatttagtgt gattctcttg tttggaaaac
     2101 tttattgaga tagaatttaa catatacata attcgataaa ggggtgtgac tatgaaaatt
     2161 agtaaattac cagaagcaga attaaaagtt atgagatata tttgggaagc taacgatgta
     2221 ttaacttcta aggaattagt tgccgcaatg gaagaaaaat acgaatggaa tgaatcaact
     2281 acattcacag ttttaaaaag gttagagcga agggagtttt taagtacaga gaaaataggt
     2341 aaacttacac actataatgc aatggtaaaa gagaaaaaat atctaagatt tgaaacaaag
     2401 gaatttttga agaacataca caagaattca atttcaagtt taatatcagc gttacatagt
     2461 aaagatgatg aagtggatga agataaatta atcgaactcg aagaacattt taaaaattta
     2521 aaagagaaag aataaataaa gattatttta ggtggcaaat tatttttgcc accttttttg
     2581 ccaccatgac agtttaataa aataaaatac aaataaagaa atataaataa aatagaataa
     2641 agaaattgtt ttagataggt tgtaaataaa tgtactcata aaaacataaa taaatataaa
     2701 taaacataaa taaaatataa gaatttctgt gagaaatgca cttaatgaac ttgtaaaaga
     2761 agatatatta tgtaaaaaac gtggtaaagg gacttatgta acaataccag agagaataga
     2821 agcaacatgt gcaggaaata gttttactaa ttcttgtcat cgtattaatg caaaaccaag
     2881 taccaaaata atctctgttt taataaaaaa agcagataag caagtagctg aagctttaag
     2941 tattgaacta gaagagaagg taatatgtat taaaagactt agattaatag atgaagtacc
     3001 tgttattttt gaagttgact attttagaat agattacatg tttttattaa aagaagaatt
     3061 agaagataaa tctttaatgg aggttatatc taataatata agcacattac ctaaacgagt
     3121 agaaaatata tttgaagtaa aacattcaaa caaagaatat tctgaccatc ttaaatgtgc
     3181 aagtaatatg ccactattga aagtaagaca atcggtgtat acagaaaata atgatgtctt
     3241 atattacaat gagcaattta taagaagtga taagtacaaa tatgcagtat cagcagaaat
     3301 ataatctata atttagtagg aggatgagta tgaaaaagat aatattagca tctcatggag
     3361 acttttcaaa agggctttta aatgcagtaa aaatgatagt aggagattta gcagattgtg
     3421 tcagttcgta tggtttgtac cctggacaaa gtgcttcaga ttttgcttta gaattagaaa
     3481 aaacaatatc agaagataaa gaatgtgaat atataatttt atctgactta tatggagcaa
     3541 gtgtatgtac agcaatgtta agattaacta atctacataa tgttaggtta ttttctggaa
     3601 tgaatttgaa tatggtatta gaacttctta caaggttttc tgatgaatta accgttgaag
     3661 atatagacca attagtggaa gaatctagaa gaggaataca aagcgtgaca ttacaaatta
     3721 aagaagaaga agaggtattt tagtttatga aaaaaaaggg aatattatca gtatttaaaa
     3781 gtaaaaaacc aattatagca atgatacact taaaaggaga tactccagaa gatatctttg
     3841 aaagagctaa aaaagagatt acaatctttg aagaaaatgg tgtagatgga ataatgttag
     3901 aaaattacta tggtaattat tatgatttag aaagaatact tgaatatgta tcaaaagcta
     3961 atttatcaat accatatgga gtaaactgtt taaatgttga tactatggga tttgaactgg
     4021 caacaaaata taatgctagt tatatacagg ttgattctgt agtaggtcat gttaaaccta
     4081 gagatgaagc tactttagaa gagtttttta aattacaacg ttcaaaatgc ccagcgtatt
     4141 taattggagg cgtacgtttt aaatatcagc cagtattatc agaaaatgat gttgaagaag
     4201 atttaaaaat aggaatgaca agatgtgatg ctatagcagt tacagaaaat gctacaggtc
     4261 aagaaacatc aatggaaaaa atagaactgt ttcgtaaaaa tttaggagac ttcccattgg
     4321 ttatagctgc tggagttaca ttagaaaatg caaaaaaaca gcttaaatta ggagatatgg
     4381 caataatagg aagttacttt aaagataatt acaaagattt tggggatgtt agtgtagaac
     4441 acgtaaaaac attcatggat gaaatcaaaa aaattaggga ggaattataa tgattaaatt
     4501 agtaagagta gaccacagac ttatacatgg acaagtagca tttacatgga caaagttttt
     4561 aagcacagac tgtatattga tagcaagtga tgacttatta aaagatgaat tgagaatggc
     4621 aggacttaaa atggctaaac catctaatgt taagttagta atgaaaagca tagcagattc
     4681 tataaaagca cttaactcag gtgttactga taaatacaac ttattgatac tttgtgaatc
     4741 tgtagaagat gtttatagac ttgctaaaga ggtaaaagct attaaatcta taaaccttgg
     4801 tggaacaaaa tcagatgata atcgtgaaaa tatatctaaa gcagtgcatg tatcaaaaga
     4861 tgatataaag atgattaaag agttagattc agaaggcgta aatgtatttg tacaattagt
     4921 tcctgatgat gatgctacaa atgtaatgaa attaatataa ttttaggggg atgaaaatgg
     4981 aatttacaca agttattttg ataactctaa ttgcattctt tgcatatatg catagttttg
     5041 taggttctac aatgcataat agacctatag ttgttgcacc acttgttggt ttagcactag
     5101 gtaatcttca tacaggtata gtaataggtt caacattaga attagtattt atgggtgcat
     5161 ttcctgttgg ggcaagtaat cctcctgatt ttgtgtctgg ttctattatt gccacagcgt
     5221 ttgtaatatt aacgggtcaa gatgtatcag cagcagttgt acttgcagtt cctatagcaa
     5281 ctctagtatt gcttatagat aacttcttaa tgacagtagt acttacatgg ggagctcata
     5341 ttgctgaccg ttatgcagaa gaggggaata ttgaaggcgt agaacgtgtg caattattat
     5401 ttggtatagg aaataaatta atattagcaa taatagtagg tataggattt tcattaggtg
     5461 ttccagtaat agaaaaaata ctttcattta taccatcata tgtaactcat ggaatggatg
     5521 ttgcagcagg cgtgattcca gcaataggtt ttgccatgtt agctagaatg atgcttaata
     5581 agaaaacagt agcattttta ttattaggat ttatattggt tgcttaccta aacataacag
     5641 taacaggagt tgcattgttt ggattagcaa tagctttaat atatgttaac tttgtaggag
     5701 agaaggaggt aatagtagat gacaacgagt tctaagaagt tagagacaat ttcaccagat
     5761 agtaaaataa cgaggaaaga cttttggaaa tgttttagaa ggtcattgac attagattca
     5821 tcttggaact acgaacgtat gcaaaatata gcatatgcat acatgatggc accaataatc
     5881 cgtagattat ataaagatga taaagaaaag aaatctaagg ctttaaaaag acatttagaa
     5941 tttatgtcag ttactcctca tataagtact cttttggtag gtatatctgg agctatggaa
     6001 gaagaaaatg caaagaataa agaatttgat gctaatagta taaatgctgt taaatcaagt
     6061 ttaatgggtc cagtttctgg gataggagat tcatttttct ggggaacatt aaagcttata
     6121 gcagctgggg ttggtatagc tttagcttct cagggtaaca taatgggacc gattttattc
     6181 cttttaataa taaatgttcc tcattttatt atacgctata tatgtctaga taaaggattt
     6241 aagtatggaa ctcaattctt taaagatgtg agtggttcaa gtatagtttc aaaagttatg
     6301 gaagcagctt caatgttagg gcttatggta attggaggta tgactgcatc taatgtaatg
     6361 ttaaaactta gtgttaatgt aggtagtgga gaatgggcag agcctattca aacttactta
     6421 gaccaaataa tgccatgtat gcttccagct atgatattcg gtattatgta ttggttatta
     6481 ggaaaaaagg taaagacaac tacgatatta atttcagtta tgattatttg tatagtattg
     6541 gctgctattg gagtagtata aataaaaaaa tcccaagttc atttgaacga gggatttttt
     6601 gactaaatta aataaaaaag aaagatggtt cataataatt tataagcatt tatgtgatag
     6661 ttataattag cttattaatt agttataaac tcaaaattaa gcactacttt attaaagtta
     6721 ttatattata atttcgaaat actcacataa tctgtttaga aatttatctt ctaatgggga
     6781 aaaagttttc tttttagagt aaatgaagaa aaactttcta gtaaaatcaa catcctttat
     6841 tttataacat tttattttac ctgcattgag ataatcgata gctgaaatgt acgatataaa
     6901 ggacactcca agacctaatc taaccatttc cttaatagcc tcatttgatt ctacatgagc
     6961 gattacattt aatttactta ctggaaagtt atttttacta agtgtgttta gtattaagtt
     7021 tcttgtacct gacccttctt ttctcataat aaaatttaga taagccaatt ctcctatatc
     7081 gatacaatta tttttattat caattttaaa atctgaagga gtgataagaa caagttcatc
     7141 atctaataag tctaggtact ctatttgagg attattgatt ttagaaccaa ctaatccaaa
     7201 gcttattcgt tcatttaaaa tttctgatat agcatattga gaatcataat ggcttatgga
     7261 aaattttaca tctggataac tcatagaaaa actcttcata aaatcaggta aaatataagt
     7321 ttcaggtatg gaactacatg ctatatctat aataccttct atttttcctg aatattcttt
     7381 tatatcataa attgcacgtt tgcaattatt caatatatat atagcatgtt cataaagaat
     7441 ttcaccagat tttgtgagtg taataacttt gttgcttcta tttataagaa cagtttctag
     7501 ctctctctct aaattctgta tgtgtgcgct tacagtaggt tgagttaaaa aaagttctct
     7561 tgcagccttt gaaaaactct gatgttttgc aacagcaaca aatacttcaa gttgtttaaa
     7621 atccattatt taatcctcct taaattatct aaaactatta taacatattt gataaattaa
     7681 tagtcaaagg tgatttttaa aatagaaaac ctttaaatat tcatggagtt tttgattgtt
     7741 taggaataat ttggtataat gttgtaaggt acttacaata aaaatatcgt aaattgaatt
     7801 ttcaaaattc aactttaaag tgttttttat tacagaaaaa ttacaattag cttcttaatc
     7861 ttatt
//
LOCUS       DAMJWS010000125         7843 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMJWS010000125 DAMJWS010000000
VERSION     DAMJWS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.130"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            346..1647
                     /locus_tag="PC399_003443"
     CDS             346..1647
                     /locus_tag="PC399_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2594265.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1723..1998
                     /locus_tag="PC399_003444"
     CDS             1723..1998
                     /locus_tag="PC399_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2594266.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3441..3671
                     /locus_tag="PC399_003445"
     CDS             3441..3671
                     /locus_tag="PC399_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2594267.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3664..4245
                     /locus_tag="PC399_003446"
     CDS             3664..4245
                     /locus_tag="PC399_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2594268.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSI"
     gene            complement(4820..5497)
                     /locus_tag="PC399_003447"
     CDS             complement(4820..5497)
                     /locus_tag="PC399_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2594269.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5686..7050)
                     /locus_tag="PC399_003448"
     CDS             complement(5686..7050)
                     /locus_tag="PC399_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2594270.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7178..7699
                     /locus_tag="PC399_003449"
     CDS             7178..7699
                     /locus_tag="PC399_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2594271.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3064 a          879 c         1353 g         2547 t
ORIGIN      
        1 tatatcaaat aagatattac tatcaagtag tgaattatat tgaacgatat attaataatt
       61 ggcagtaagt tatatcaaat aagatattac tatcaagtag tgaattatat tgaacaatat
      121 attaataatt ggtagtaaag tatatcaaat aagatattgc tattatgtag taaattatat
      181 aaaacaatgt atcagaataa atagtatatt aataatttgt agtatatcat gacgaataat
      241 acagtcataa caaatagtga gtcataacaa ataatgtatt tataattgat aatgtattta
      301 aaaatcaaaa tataaaaact tataaggaaa gagagattaa tttatatggg taaatcaaaa
      361 gatgttgaaa tagagaagtc tattttaggc acaattcttt tagacaataa attaagctat
      421 aagctagatg aattaaatga aaacatgttt atgaatgata tatgtcttga gatatttaaa
      481 attatgaaag aacttaaaaa agaaaacata gtgattgatg tggccactgt gaaatcaaag
      541 atagatagaa aatctctagc aataaaaacg agtgatgtga ctaatctaat tacttggggt
      601 cagaattttg gtcttgatgg tcatattaaa attttaaaag aaaatttagc tagaagaagc
      661 ataaatcaaa attgccaaaa ccttttacac agcctaaacc ttggagaaaa tatcgatact
      721 tgtatttaca aatttgaaag taatataaag gagattttgg acaaggatac ttatgaaaat
      781 gatgatgtaa atagtatagc agggaaggtg cttgattttt tagaaaacaa aaaagatatt
      841 ggatttaaat ttggtataaa attacttgat accactatag gaggtctttt taaaggagag
      901 ctaaccacaa tagcagctaa atcgggggtt gggaagactg ctttggcact tcaaataatg
      961 ttaaattcat ttaagcaagg taaaaaaact ttatttataa gtagagaaat gaccagtgag
     1021 caagtattta tgagaaatat atgcagagta actggtgtaa gtacaaggaa tatgaaatct
     1081 aaagaaatag atgaaaatga ttggaaactt attgtaaatg ctataggtga tttaagcgaa
     1141 aataacttaa tttatattaa tgataagata gatacaatta gtgcaattag aaagaggata
     1201 aggcaggtaa agccagattt attaatagtg gattatgtgc aactccttac atctcagaaa
     1261 agcatggata agagagaaag ggaggtagct actttttcga gagaacttaa aaatatgact
     1321 ttagatttta atatacctgt aatccaatta agtcagttaa atgatgaaat gaaagattca
     1381 agaccttatg gagatagacc tatgagggat agcaaagcca tctaccacga tagcaacaat
     1441 gtggtttata tacatcaatt aaaggggagt gattatgaag aagcagtgag agatattgga
     1501 gaaagtgagg aggcagttag agcatcagaa tacaggggaa ttaagatggt tgatttaata
     1561 gttgcgaagt gtagagatgg acagactagg cataagcatt tttgttactt tggggataag
     1621 ttacattttc aagaactaaa ttattaggtc aataaagtag taataacaag cttattaaaa
     1681 gtcgggaaat agcaaataga ttataaaata tgaggtgaaa atatgttgga attaagaaaa
     1741 attatagaat tacttgaaga acacaagata gaagtaagta ttccaaagga gttacataag
     1801 tatagtttat tggaatacct tgaattgtgt caacaaagtg ggggaacata tttttatatt
     1861 aaaacactgg aaggggcttt aaaagaggct agaaatgagc ttatgattaa attgtttaat
     1921 gggaaaaatt atatggaatt agcgaggatg tttgaaatga gtgttattaa tgtcaggcat
     1981 attgttagaa aaaaatagat atttttggag aatacaagtg tgatatactt ctgaaataga
     2041 agttattata gttgaaataa aattgtggtt aataaattaa agttgtgtat aagtttaaga
     2101 ttatagagga gtataagtgt gattaacagc atgattttgt gaataaaatt gaggatatat
     2161 atgagttaaa tgtgtgatta ataaggtaag gttgtgtata agattatgag tatttaaaag
     2221 gaggggctat aattaatagt gtagagttgt gtgtttgctt aagattatat aataatttaa
     2281 tagattaaac tatactaata aggtaaaatc gtgtgtttag ttaaagctta ttataaaatc
     2341 ttgatatgtg attaatgatg caatatgtga ataaagttaa gattataata gatttaacct
     2401 gtggctaata gaataaaatt gtgaataaag ttaagattat aataggttta acctgtgatt
     2461 aatagagtaa gagtgtaaat agagataaac ttcaatatga gcttaaccta tgattaatag
     2521 agtaagagtg taaatagaga taaacttcaa tatgagctta acctatgatt aatagagtaa
     2581 gagtgtaaat agagataaac ttcaatatgg gtttaacttg tgattaatag agtagggtgt
     2641 aactaaagat aaacttcaat atgagtttaa cttgtgatta atagagtaag gtgcaactaa
     2701 agataaactt caatatgagc ttaacctatg attaatagag taagaatgta aatagagata
     2761 aacttcaata tgggcttaac ttgtggttaa tagagtaaga gtgtaactaa agataaactt
     2821 caatatgggt ttaacttgtg gttaatagag taagaatgta aatagagata aacttcaata
     2881 tgagcttaac ttgtgattaa tagagtaaga gtgtaaatag agataaactt caatatggga
     2941 ttaacttgtg attaatagag taagagtgta actaaagata aacttcaata tgagcttaac
     3001 ctatgattaa tagagtaaga atgtaaatag agataaactt caatatgggt ttaacctgtg
     3061 gttaatagag tagggtgtaa ctaaagataa acttcaatat gagtttaact tgtggctaat
     3121 agaataagat tgtgaataaa gttatagttt tagatgggta ttaacctgtt actaatagaa
     3181 tatgattagg tgtaaaatta tagagctgta taagaaaata ttatagatat attaaatatt
     3241 agataataaa tatttatata gatacacaaa agaaaaggcg atatgaagct aaacttaatg
     3301 acttcatatc gttttttatt tgaaactcct ttgtatttag agtgtgattt ttaatattga
     3361 ggttttatgt gattaagtca cagataaaaa gtaagtatct tgatataatt cttatagatt
     3421 aataatatag ggaggttatt atgtctaata aaaaaaagaa gtatgcaaat attttagcaa
     3481 atgagtgcgt tgcttgtggt agctgtataa aagcatgtcc aagaagtgct atctcagttc
     3541 catgtggcat tagtgcaaaa attaatagag atttatgtgt tggatgtggg atttgtgaaa
     3601 agatatgtcc agcatctgta attgagatta taacaatatt aaaagaggaa gaaggtaagt
     3661 gtcatgaata ataaaaaaca ttggtatgac tatttatgga ttttttcttc aatatatttg
     3721 atattgggat ttgtaaatat tattttcgct tggattgggc taatttgttt ctttgttcca
     3781 ctagctattt ctattgttaa gggaaataaa gcttactgta ataagtattg tggaagaggg
     3841 caactgttta atttgcttgg aaataaattg aagctgtcaa gaaatagaga tattcctaaa
     3901 tttattagaa gtaagtggtt tagatatggg tttttgacat tttttatggt aatgtttgcc
     3961 aatatgcttt tttcaactta tttagtgttt gaaggaagta gaaattttaa agaagtagtg
     4021 actttgttgt ggactataaa aatgccttgg caatgggcgt atcatggaac tttagtttca
     4081 ccttgggtgg tacagtttgc atttggtttt tatagtgtta tgcttacatc aactgttctt
     4141 ggtttgatta ctatgatatt gtttaaacct cgttcatggt gtgtttattg ccctatgggt
     4201 actatgactc agatgatatg taaggcaaag agtaagtcta tatagaaagg ctttatgtga
     4261 tatttacagt tttgttagag atgagtagta ggtttgaata tatatgacaa atatatgagt
     4321 ataggggcat agatttagtt tgtgtaaggt aaactgattt atgcttcttt tttatgtctt
     4381 agattattgg ctaagtggaa acaatgaatt tagatattaa gaattattca gttatttgaa
     4441 gaaaatagaa taataattaa cattatatga atggatattc attacatata agccagttaa
     4501 tagcaagatt tatttaatat gaaataggtc aagtatttaa actaaataag gtcatgacaa
     4561 ttaatataag tatagatata aaaagattag aagttataac ttataaaagg tattctatat
     4621 aaatagtatg tggaaaatag tcaaattaac ttattagaaa caaaaaatat acataaaaaa
     4681 ataaatgtaa atatcaaata agatactata taaaaataga tataagtatc ttttttatta
     4741 ttgaaattat gaaaatatat ttgatttcac acaaaaaaga gtgcctcatg aactaaaaag
     4801 ttcattttga gacacacttt tacagtgtca aagaacgaag tttatcctta tccaatactt
     4861 ttataccacc acgagataaa gacacaatac cttctctagc aaaatatttc aacattctcg
     4921 atacaacttc tctggcacta cccatatact tagcaatttg ctcatgagtc atatttatag
     4981 tgctactgcc atttttagct atctcatcaa ttaaaaaaga tgccaaacgc ttatcaaaac
     5041 tcataaaaag aatttgttgc atagcccaca taacatcaga aaaacgttcg gtagctaatt
     5101 tatatgaaaa taactctgca tgaatatttt ctgtagataa ttttgcaaaa acagaggaac
     5161 taatctgtat aatatcgcag tccgtttcag catctacata gacatcaaaa gttattgttt
     5221 ttagaataca ggaagctgat aaaatacata catctccatc atcaagtcgg aacagtgtaa
     5281 cctctcgacc ttcatctgaa agtatgtatg tacgtactgt tccagacttt ataatcaaaa
     5341 taccaataca gtcactatca ccacaatgta ctggattccc ttttttgtaa tgcgaggtat
     5401 ttgctgaaga aattaataat tctttttgta agtcagtaag tttatcccaa aaaggcaagc
     5461 tatgtgtaag tatatcgatt tcattttgtg aaatcataca atatctcctt tcttaaattg
     5521 ttataatccc atgatacaag tgaatagata aattgacaag tagtttgtta ataggattat
     5581 ttagtcagat agtaatagta tgatttatgt agaaaaaata tgataatata tattcttgct
     5641 attaatgaga aggtaatatg ttttgatgat agtaggtttg tataactaat gcataccgtt
     5701 tatttttaaa agattaagtt ccttatgtaa ccttatagac ataaaaccag caattatagc
     5761 agccatcaaa tcagctattg gttgtgcata taagatacca tttaacccat agaatacagg
     5821 aataatcaaa attataggga taaaacagac accttgtcta cacataccta aaaatccacc
     5881 ttcctttgct tttcctagtg cgaggaataa tgtagaatac actgtataga atccaaatag
     5941 cataaatgaa aacccatttg ccctaagagc tttactacca ttttctatta atagaatgtc
     6001 accacttgta aattgtgaaa ttatagattt tgggaagagt atcaatataa gaccaagcac
     6061 tacacaaaat attgtagtcc ataatatgga agtttttatt gctatatgta atctatcgta
     6121 gttctttgaa ccaaaattat aaccagcaat tggttggaaa ccttttataa aaccaaatac
     6181 aacaagacta cctaaagacg ttattcgtgt tacagcaccc attccagcta tcacagaatc
     6241 accatacttt gtagcctcca tattagttaa tgtaatggca agactagtca ataattgaaa
     6301 tattaaggta ggtatgccaa tttttaggat ttctgaaaaa atttcttttg aaaaacagca
     6361 ttcgttaatt ctaaaactaa atatactttt cttgcttaaa atatatccta cataaacaag
     6421 tgttgaagca atctgtgaga tagctgttgc aatagaagca ccccttaccc ccagaccaaa
     6481 agtataaatg aatattgggt ctaaaatcat attaagcaaa gcacctaata acatagcaca
     6541 catagttgtt ttagcagctc cttcacttgt gacaatatta gtcatagtga cattgaatat
     6601 attaaaaata gatgacacga cataaatgct agcataagat attgcataag gcataatact
     6661 ttttgtagct cctaacaatt ttaaaattgg attgagaaaa atcataatca caataataac
     6721 taatgtaccc actataatac tactgtaaat agatgtactt accacttttc ctgctttttc
     6781 attatcacca tggcctaaaa gtctggcaat atatgaagca gctccatttc caaacaataa
     6841 tcctaatccc acaacaagct gacctaatgg aaatgcaacg gatatagctc ccatttgact
     6901 tgttccaagt ccacccacaa agtatgcatc aaccaaattg tataatgcag ttatcatcat
     6961 acctatcatg gtaggtaatc ccatagataa caatgctttt gagattgggg cagaccctaa
     7021 taattcaatt cttttgttac tttctttcat acttaacact cctcttctat tgacattaag
     7081 tctaaattat aatactataa attatagtaa gtatggaaaa tagtactata atttatagta
     7141 tttgtcaata gtaagaaaac taaaggagat gatttttatg gctacaatag atttaattgt
     7201 attgggaata gtaaagcaac aacccaagag tgcttatgat attcaaaagg cagtggaata
     7261 caggaatata tcacgttggg ttaaaataag tacaccatca atttataaaa aagtaattca
     7321 attagaacaa aagggatata ttgaaagtaa aactgtgaag aatggaaaaa tgccagaaaa
     7381 agcagtttat tcattaactg aattaggtga gacacatttt ttagatttaa tgtttgatac
     7441 atctagcaaa ccaataaata ttcttttaga ttttaatgct gttatagtaa atttaaatag
     7501 ccttacacca gaaaaacaga aattatgtct caataatatt gaagagaatg ttcaagagtt
     7561 aaagaaaact attgaggaaa atataagtat aaagacagaa gtaccagaca cagcaaaagc
     7621 agtgttggag caacagttgg tgctagcaca agcattggaa acctggataa attctttaaa
     7681 agataatatg ggaaattaga attagagaag ctaacttaat aatcattaaa caaaaaccag
     7741 catatttaat aatgagatat tagaaaaaat aaaacaaatg atataaactg aaaagtatat
     7801 ttaaaataaa gctcacttaa ttgagggaga atgttaaact ttt
//
LOCUS       DAMJWS010000126         7822 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMJWS010000126 DAMJWS010000000
VERSION     DAMJWS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.131"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            103..1554
                     /locus_tag="PC399_003450"
     CDS             103..1554
                     /locus_tag="PC399_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2594272.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1752..2393)
                     /locus_tag="PC399_003451"
     CDS             complement(1752..2393)
                     /locus_tag="PC399_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2594273.1"
                     /translation="MTDNYIFDSYNNLVDGLSAYLGEGYEFILYKFNGVHYSINKIAN
                     GKYNTVKEGDIITDFDLALINKLENDADGDFISYFNRNKLGEPLKSTDIVIRGVDNKI
                     IGLLCIHFYLNTPLTRIIGSYIPEHNAMQSAPVLENIVEDTDEVILKSYQDAKVIVEN
                     DSNISSNDKNKSIVSILNESGIFKIKDSVVKVADLMGLSKNTIYMHLRNLNKN"
     gene            2867..4246
                     /locus_tag="PC399_003452"
     CDS             2867..4246
                     /locus_tag="PC399_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2594274.1"
                     /translation="MAQDKIVVEILENIGGKENIKEVAHCMTRLRIKVCDSSQVNQEK
                     LKKIKGVLGLVLKGEQYQIVIGPNVSQVYNTLNEVIGIIPEKDSKKTISEGKQNSIAK
                     ILDTIAGIFNPIVPALAGAGLIKAILALLVASKFLSADSETYIILNTISDGVFSFLPF
                     MLAYSAAKIFKMNTYVAVAIAGAMLHPNFINLIQQGLTKISFIGIPMSLIQYNGSVFP
                     IVLTIWFASYIEKGVDKITPKALKIILVPALTILFAAPIALIIIGPIAQMLGNYIAGG
                     ASILFERGGIFAGLIYGGIYSFMVVTGLHHGMVPVLVDGITRYGFNYISPVSGSSNMA
                     QAGSAFGVWLKAKDKDLKAVAATATISALTGVTEPAIYGVNLRLKKPFLCAAIGGAAG
                     GGFAAYFQCKAFAMGGPSFLTLPMFIGEGGHVGLVAIAFTIAFIVSAIATYIVGFEEE
                     VTADSHANL"
     gene            4290..5024
                     /locus_tag="PC399_003453"
     CDS             4290..5024
                     /locus_tag="PC399_003453"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010990049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2594275.1"
                     /translation="MKIYLTKDYEEMSQVAAQMVLKYMYSNDNKRINLSITAGQTPKR
                     TYEILSEFVKNKPYFENVHYYNFDEIPIKGEKVGVTIRNLTNMYFREAQIPEERIHVL
                     DENNYQGFDRKIEADGGIDMVLMGLGWDGHFCGNLSGTVNSFDEETRAVSTYITDDMA
                     KLLTKSVGGNPEKLFDYYVTYGPKTIMHCKNIIMIVNGKHKAQILKQTLFGPLDLLCP
                     SSLLRLHPNFTLIVDEEAASEIKDLI"
     gene            5113..6312
                     /locus_tag="PC399_003454"
     CDS             5113..6312
                     /locus_tag="PC399_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2594276.1"
                     /translation="MYNHMKKVFQGLEGGLFSSVEKADVGDSYQKLGEQGVSLMGWAD
                     PFFPDYCIPRHILDAATLAISSNMSTHYTAPVGNDLLKIEIAEKLKEVNNLDVDFKRN
                     ILITPGSDSGLYYSMLPFINEGDEVLIPTPCYPNNIQNTKLMGGVPIFVELKESDGYQ
                     IDITEFEKRLTNKTKLVVLTHPNNPTTTIFNKESLEKLAKFIIDNDLILICDQAFEDF
                     TFENELITPASLEGMFERTVTIFSTSKGMGLSGFRVAYMVCSDKLMDKYYGAAVTVLG
                     ATNTVAQMSTIEAFKDRSFMEDFERVFDKRRQEAYKIINEIPNVSMQMPESGFLGWIN
                     VSKLGTSSEIQKYLVDEAKVCINDGINYGPGGEGHLRIILGSYKDDSKVIGALNRIKD
                     ALIKKQQ"
     gene            6336..6818
                     /locus_tag="PC399_003455"
     CDS             6336..6818
                     /locus_tag="PC399_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2594277.1"
                     /translation="MLSIFKSKKNDNKLKAFASGKVIPIEDVDDNVFSSKMLGDGIAI
                     YPTNEYVVAPCDAEVNIIMKDTKHAVGIKLFNGMEILFHVGIDTVSMNGEGFEIFVSP
                     GDKIKRGDKLISFNSKLIKEEGLQDVVIMVVTNSEQYKNIKYHIGLEAVIDDTIIAEI
                     "
     gene            complement(7136..7733)
                     /locus_tag="PC399_003456"
                     /pseudo
     CDS             complement(7136..7733)
                     /locus_tag="PC399_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2886 a          907 c         1341 g         2688 t
ORIGIN      
        1 atattataat taaattaatg aatttttaac aaggaattat tcattttttt gattttaaga
       61 tttattgaga aaacaaaact aaataggggg gtaattaata taatggattt tttcaatggt
      121 attttaacga gtattaacaa tgcagtagta tttgtaaaca attacttatg gtcgtatatc
      181 ttaattataa tgttagttgt aattggtatt tactttacta ttaaaacaaa ttttgtacaa
      241 ttcagatatt ttatagaaat gtttagactt ctgggtgatg gtacagcgaa taaaaatgca
      301 aaaaaggaag gaaaaatctc ttcatttcaa gcattttgta taagtacatc ttcaagagtt
      361 ggaactggaa atatagctgg aatagcaatt gcagtggtag caggaggacc tggtgctgta
      421 ttttggatgt ggcttatagc attaattggt tctgcatcaa gctttgttga gagtacatta
      481 gctcagattt acaaagtgaa agatggtcaa gcttttagag gtggaccagc gtattatatg
      541 gaacaaggtc taaataaaaa atggatgggt attttattct caatacttat aactatatgt
      601 tatggttttg tatttaatgc agttcaagca aatactgttt cacttgcatt taataatgca
      661 tttggaataa gtaaaatggc tatgggaata ggtcttggaa ttttaactgc aatagttatt
      721 tttggtggag tgcatagagt tgcaaaagtt tctgaaataa tagttcctgt atttgcagga
      781 ttatatatat tagttgcttt ggctatagtt gtaatgaata taacagagat accaagtgtt
      841 atagcattga tagttgaaag tgcatttgat tttaaaggaa tggcgattgg tacatttatg
      901 ggagttgtaa tgactggagt gaagagaggt ctattttcta atgaagcagg tatgggtagt
      961 gctcctaatg ctgctgcaac agcacatgta actcatcctg taaaacaagg tttaattcaa
     1021 tctttaggtg tttttacaga tactataata atatgtagtt gtacagcatt catggtatta
     1081 ttatattcag gttatagtag tagtggagca actggaattg aattagctca agaagcttta
     1141 actcatcata taggaccagt tggaaatata tttatagcag tttgtatatt cttgtttgca
     1201 tttagttcta tagttggtaa ttactattat ggtgaatcaa atatggagtt tatgagtgga
     1261 agtaagacta aacttaatgt atttagagtt tttgttgtag gtatggtttt atttggttca
     1321 cttactcaag tggatgtagt ttggaatctt gcagacttat ttatggcatt gatggcgata
     1381 ataaacttaa tagcaatagc acttttagga aaatatgcat ttattgcttt aaaagattat
     1441 acaagtcaaa aaaaatcagg aataaaagac cctgtgttta ttgcagatga aatagaaggc
     1501 ctagaaaagg tatcagagtg gcatagggaa ttatctgagg aacatttagg ataaaaaata
     1561 gtataaaatt ataaaaccat tttgaaagac tagagtatgt atatacttta tatgaatata
     1621 atattgaatt aatttatatt actggaattt ttaattccag taatattttt ttaattacaa
     1681 ggatattatg atgacttaaa gactgtggaa aactcttttt tagttttcat tattaatatt
     1741 aattgacaaa tttagttttt atttaaattt cttaagtgca tataaatcgt attttttgat
     1801 aatcccatta agtctgcaac tttaacaaca gaatccttaa ttttaaaaat accactttca
     1861 tttaaaatag aaacaataga tttattttta tcattgctag atatattaga atcattttca
     1921 acaattacct ttgcatcttg gtatgatttt aaaattactt catcagtatc ttctacgata
     1981 ttttctaaaa caggagcact ttgcatagca ttatgttccg gaatatatga accaattatt
     2041 cttgttagtg gtgtattaag ataaaaatgt atgcagagta gaccgattat tttattatca
     2101 actcccctta tcactatatc tgttgatttt aaaggttcac ctagtttgtt tctattaaaa
     2161 taactgataa aatctccatc agcatcattt tcgagtttat taatgagtgc caaatcaaaa
     2221 tcagttataa tatctccttc ttttacagta ttatatttac cattagctat tttatttatt
     2281 gaatagtgta ctccattaaa tttatataaa ataaactcgt atccttctcc taaatacgca
     2341 gataatccat ccactagatt gttataagaa tcgaaaatat agttatcagt catgttaaaa
     2401 gtcatatgtt acctcctaag aaatatctta atatcatatt aacatataaa atgaatttaa
     2461 taaaatatat ttcagtgaaa taataaatat aatgaaaaaa aaactattga ctgaaaaata
     2521 tttcagtgat attattaaaa tacagtaagg caaacgtatt attgacagaa tatataaaat
     2581 gttaaaaata gtatctagga attaaacaaa ttaaaggact agtgatttga atttttttag
     2641 tataatatgt gacttccaat taatatttat taaacaccaa taaaattgta aatatgctta
     2701 atgatatact ttagcgatga agtgaaaata ttttattttt atgattaacc tataggaatt
     2761 aatttagcat actgtttagc tagtaaatct aaataaattt tagctttagc tgatatcatg
     2821 ttgaataaag tttctattgg attttgtata acaaaggagt ggggagatgg cacaagataa
     2881 aattgtggtt gaaattttag aaaacattgg tggtaaggaa aatattaagg aagtagcaca
     2941 ttgtatgact agactgagaa taaaggtatg tgatagttct caggtaaatc aggaaaaact
     3001 aaagaaaata aaaggtgtac taggtcttgt attaaaaggt gagcaatatc aaattgttat
     3061 aggtcctaat gtatcacaag tgtataatac tcttaatgaa gttataggaa ttatacctga
     3121 gaaggatagt aaaaaaacaa tatctgaagg taaacaaaat agtattgcaa aaatattaga
     3181 tactattgca ggtattttta atcctatagt tccagcatta gcgggtgcag gtctaataaa
     3241 agctatatta gcattactag tagcatcaaa gtttttatct gcagatagtg aaacctatat
     3301 aatattaaat acaatatctg atggtgtatt ttcattttta ccatttatgt tagcttactc
     3361 agcagctaaa atatttaaaa tgaatactta tgttgctgta gcaatagcag gagcaatgct
     3421 acatcctaat ttcattaatt taattcaaca aggcttaact aaaatatctt ttatcggtat
     3481 tccgatgtca ctaatccagt ataacggaag tgtatttccg atagtactaa ctatttggtt
     3541 tgcatcgtat atagaaaaag gagtagataa aataacacca aaggcactta agataatatt
     3601 agtacctgca ttgactattt tatttgcagc tcctatagca ttaattatta ttggaccaat
     3661 agctcaaatg ctaggaaact atatagccgg tggagcaagt atcctatttg aaagaggagg
     3721 aatatttgca gggttaattt acggaggaat ttattcattc atggtagtaa caggattaca
     3781 tcatggtatg gttcctgtat tagtagatgg aataacaaga tatggattta actatataag
     3841 tcctgtgtca ggtagttcaa atatggctca agcaggatct gcatttggag tatggttaaa
     3901 agcaaaagat aaagatttga aggctgtagc agctacagct accatatcag cattaactgg
     3961 agttaccgaa cctgcaatat atggtgttaa tttaagatta aagaaaccgt ttttatgcgc
     4021 tgctataggt ggtgcagcag gaggtggatt tgctgcatat ttccaatgta aagcatttgc
     4081 tatgggaggt ccatcgtttt taactcttcc tatgtttata ggagaaggtg gacatgtagg
     4141 attggtagca atagcattta caatagcatt tatagtatct gcaatagcta cctatatagt
     4201 aggatttgaa gaagaggtaa cagcagattc acacgctaac ttataataaa gagaaattat
     4261 ttaacatatc aaaaaggaga ttaattacta tgaaaattta tttaactaaa gattatgaag
     4321 aaatgagcca agtggcagca caaatggtgt taaagtatat gtacagcaat gataataaaa
     4381 gaataaacct ttctataaca gcaggtcaaa caccaaaaag aacatacgaa atattaagtg
     4441 aatttgttaa gaataaacct tattttgaaa atgtacatta ttataacttt gatgaaatac
     4501 caataaaagg cgaaaaagtt ggagttacta taagaaattt aactaatatg tactttagag
     4561 aagctcaaat accagaagaa agaatacatg tgttagatga aaataattat caaggctttg
     4621 atagaaagat agaagccgat ggtggaatag acatggtatt aatgggatta ggttgggatg
     4681 gacacttctg tggaaatctt agtggtacag tcaactcttt tgatgaagag acaagagctg
     4741 tttcaactta tataacagat gatatggcta aactattaac aaaatctgta ggtggaaatc
     4801 cagaaaaatt atttgattac tatgtgacat atggacctaa aacaataatg cattgtaaaa
     4861 atataataat gatagtaaac ggaaaacaca aagctcaaat attaaagcaa acactatttg
     4921 gaccattaga tttattatgt ccatcatctt tattgagatt acatcctaac ttcacattaa
     4981 tagtagatga agaagcagca tctgaaataa aagatttaat ataaataagt aaggaattaa
     5041 aattaaaaat gaatttaggt gcaaagtaat attttatttt gcacctttgc tttaataaaa
     5101 aggagaataa aaatgtataa tcatatgaaa aaagtattcc aaggattaga gggtgggtta
     5161 ttttcaagtg tagagaaagc tgatgtaggc gattcttatc aaaaattagg tgagcagggt
     5221 gtaagtttaa tgggatgggc agatccattt ttcccagatt actgtatacc tagacatata
     5281 ttagatgcag ctacattagc gatatcaagt aatatgtcaa ctcattatac agcgcctgta
     5341 ggtaatgact tattaaaaat tgagatagca gaaaaattaa aagaagtaaa taatttagat
     5401 gttgatttta aaagaaatat attaataact cctgggtcag actcagggct ttattactca
     5461 atgttaccgt ttataaatga aggggatgag gttcttatac caacaccatg ctatccaaat
     5521 aatatacaaa atactaaatt aatgggagga gttcctatat ttgtagagtt aaaggaatca
     5581 gatggatatc aaatagatat aacagaattt gagaagagat taactaataa gacgaagtta
     5641 gtagttctta ctcatcctaa taatccaaca actacaatat ttaataaaga atctttagaa
     5701 aaattagcta aatttattat agacaatgat ttaatattaa tatgtgatca agcatttgaa
     5761 gattttactt ttgaaaatga attaataaca ccagcttcat tagaaggtat gtttgaaaga
     5821 acagtaacaa tattttcaac atcaaaaggt atgggattaa gtggatttag agttgcatat
     5881 atggtatgca gcgataaatt aatggacaaa tactatggag cagcagtgac agtattaggt
     5941 gctactaata cagtagctca aatgtcaacg attgaagcat ttaaagatag aagctttatg
     6001 gaggattttg aaagagtttt tgataaaaga aggcaagaag catataagat aatcaatgaa
     6061 atacctaatg tatctatgca gatgcctgag tccggatttt taggttggat aaatgtatca
     6121 aagcttggaa catcatcaga aatacaaaag tatctagttg acgaggctaa agtttgtatt
     6181 aatgatggaa ttaattatgg tccaggagga gaagggcatt tgagaataat tttaggaagc
     6241 tataaagacg atagtaaggt aattggtgca ttaaatagaa taaaagatgc tttaattaaa
     6301 aagcaacaat aaataaaaaa taattggagt ggagtatgtt atcaatattt aagagtaaaa
     6361 aaaatgacaa caagcttaag gcatttgcat cagggaaagt aattcctata gaagatgttg
     6421 atgataatgt tttttcatct aaaatgttag gtgatggaat cgctatttat ccaactaatg
     6481 aatatgttgt agccccttgt gatgcagaag taaatataat aatgaaagat actaaacatg
     6541 cagttggaat taaattattt aatggtatgg aaatcttatt tcatgttgga atagatactg
     6601 tatctatgaa tggagaaggt tttgagatat ttgtttcacc aggtgacaag ataaaaagag
     6661 gtgataaatt aatatctttt aatagtaaat taataaaaga agaagggtta caagatgtag
     6721 ttataatggt agttactaat tcagaacagt ataaaaatat aaaatatcat attggactgg
     6781 aggcagtaat cgatgatact attattgcag aaatttaaaa acagttattt aagtaaatac
     6841 ttagataatt tagatatagt taaaattaaa tccttaaaat caaattattt aatttttgtg
     6901 taattttaac tataaagccg attaataata taaagtatac taaataatat ggttattatg
     6961 attgttactg acaaagccta agtagatata acaatttgct ctaattgagt attgctatat
     7021 ctacttgttt ttatatttaa aataaaagag tctgcattga aaagataatt ctgatgagag
     7081 taaatagggt atttaataat agtttaattc tgacttatca agatgaacct attcatatac
     7141 ctaaaacatc tttaacacct tctaaattca tacaaatagc tgtataggct gccttattga
     7201 ttatttttcc gtcaattttt actttgtagt gaacagcata tataaatatt atcgaataga
     7261 ttgtatcaag agctctattt tgctattcat tagctaaaga tagtactctg tcagttattt
     7321 ttgaaactaa tgcatgagat atatcaattc cgtaaatatt atttaaatgt atgatatata
     7381 tcgcgagtcg acattccttt tgagtacatg ccaattactt ggttctctat tccagaaatt
     7441 tcattttcgt aattttgaat aaccctaggc tggaaatcat ccctagggat atttaatttt
     7501 acctctccaa aatctgattt aacattctta gctctatatc catttcttga atttgatgtt
     7561 gctttacttt ggttatcata tctctcgtac attaaatgat cttcaatctc tgactctaac
     7621 attttgtgaa ttgtgcttgc gaataaatct tttaacatct cttggatgtc tttagtattt
     7681 tttacatcat actcttgaac taagtgtttt aatatttaat ttttgttgta cataaaaaat
     7741 gtgtacctcc tattttggta attagattta atatcttgac ttttaggaga atacacattt
     7801 tattttacag accctctttt ct
//
LOCUS       DAMJWS010000127         7620 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMJWS010000127 DAMJWS010000000
VERSION     DAMJWS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.132"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            9..83
                     /locus_tag="PC399_003457"
     tRNA            9..83
                     /locus_tag="PC399_003457"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41..43,aa:Gly,seq:gcc)
     gene            108..184
                     /locus_tag="PC399_003458"
     tRNA            108..184
                     /locus_tag="PC399_003458"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:142..144,aa:Arg,seq:tct)
     gene            194..269
                     /locus_tag="PC399_003459"
     tRNA            194..269
                     /locus_tag="PC399_003459"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:227..229,aa:Gln,seq:ttg)
     gene            278..353
                     /locus_tag="PC399_003460"
     tRNA            278..353
                     /locus_tag="PC399_003460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:311..313,aa:Lys,seq:ttt)
     gene            356..444
                     /locus_tag="PC399_003461"
     tRNA            356..444
                     /locus_tag="PC399_003461"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:390..392,aa:Ser,seq:tga)
     gene            448..523
                     /locus_tag="PC399_003462"
     tRNA            448..523
                     /locus_tag="PC399_003462"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:481..483,aa:Phe,seq:gaa)
     gene            530..603
                     /locus_tag="PC399_003463"
     tRNA            530..603
                     /locus_tag="PC399_003463"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Met,seq:cat)
     gene            618..694
                     /locus_tag="PC399_003464"
     tRNA            618..694
                     /locus_tag="PC399_003464"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Ile,seq:cat)
     gene            712..788
                     /locus_tag="PC399_003465"
     tRNA            712..788
                     /locus_tag="PC399_003465"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:746..748,aa:His,seq:gtg)
     gene            797..872
                     /locus_tag="PC399_003466"
     tRNA            797..872
                     /locus_tag="PC399_003466"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:830..832,aa:Lys,seq:ttt)
     gene            880..953
                     /locus_tag="PC399_003467"
     tRNA            880..953
                     /locus_tag="PC399_003467"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:912..914,aa:Cys,seq:gca)
     gene            961..1037
                     /locus_tag="PC399_003468"
     tRNA            961..1037
                     /locus_tag="PC399_003468"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:995..997,aa:Arg,seq:acg)
     gene            1050..1125
                     /locus_tag="PC399_003469"
     tRNA            1050..1125
                     /locus_tag="PC399_003469"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1083..1085,aa:Val,seq:tac)
     gene            1201..1614
                     /locus_tag="PC399_003470"
     CDS             1201..1614
                     /locus_tag="PC399_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594278.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1923..5354
                     /locus_tag="PC399_003471"
     CDS             1923..5354
                     /locus_tag="PC399_003471"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF2594279.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5596..7524
                     /locus_tag="PC399_003472"
     CDS             5596..7524
                     /locus_tag="PC399_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2594280.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3025 a          938 c         1518 g         2139 t
ORIGIN      
        1 ataaacgagc gggaatagtt cagtggtaga gcgcaacctt gccaaggttg aagtcgcgag
       61 ttcgaatctc gtttcccgct ccaaatttta tgtttatgtt gtaatgtggg tgcatagctc
      121 agctggatag agcaacgccc ttctaaggcg tgtgtccggg gttcgaatcc ctgtgcgctc
      181 accacatata tattggggat tagccaagtc ggtaaggcac atgactttga ctcatgtatg
      241 cgtaggttcg agtcctgcat ccccagccaa acagtatggt ctattagctc agtcggtaga
      301 gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca ccaatggaga
      361 ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa ggggccgtgg
      421 gttcgaatcc caccctctcc gccaaatgcc cagatagctc agtcggtaga gcaggggact
      481 gaaaatcccc gtgtcggtgg ttcgattccg cctctgggca ccaaaatgtg gcggtatagc
      541 ttagttggct agagcgttcg gttcataccc gaaaggtcat aggttcgact cctattaccg
      601 ctactaaaat ttaatgtggg ccattagctc agttggttag agcgcccggc tcataaccgg
      661 taggtctggg gttcgagtcc ctgatggccc accacattaa attaacttat ggtgggtata
      721 gctcagttgg ttagagcgcc agattgtggc tctggaggtc gtgagttcga ctctcattat
      781 ccaccccaac ttatatggtc tattagctca gtcggtagag cacctgactt ttaatcaggg
      841 tgtcccgcgt tcgagtcgcg gatagatcac caaaaaggcg gcgacatagc caagtggtaa
      901 ggcagtggac tgcaactcct tgatccccag ttcgaatctg ggtgtcgcct ccaataaaat
      961 gcgctcatag ctcaactgga tagagtgtct gactacgaat cagaaggtta ggggttcgag
     1021 tccctttggg cgcaccatat gaatagcgag ggattatagc tcagctggga gagcacctgc
     1081 cttacaagca gggggtcaca ggttcgagcc ctgttaatcc caccagtgca aacctctagt
     1141 aaaactacta gaggttttat ttttgattga aaaagttttt atatttagga ggtaaaaaat
     1201 ttgtatatag taataggaaa tgaaatagtt gatagtgaag aacttaaaat aattatagat
     1261 aaaaatagtg aatttaaagt tgaaaaagat ttaagtaaaa gcacaaaaag agaagatgta
     1321 atagcatatc agttaagtat agatttaaat tacctagatt ctcttataaa tgagcaatgt
     1381 aatttagcca gtctatctga tgaagagaaa tttgatgaat atatgacttt gtctgatgaa
     1441 cttgcactgg atttagaaga gttaatgcca aaatatacaa taataaatgc tagagcatat
     1501 aaattagacg aggtagatgg aattgtaaaa ataatactag ctgtagcgta tgcagattta
     1561 gggcacttaa aactttctga tgtagtaaag aggctatcta gacaagtaga ttaggatatg
     1621 aatatgaact atattaaaaa aatgtataaa atcttacgta aagatgaaaa taattatgta
     1681 aacatagaaa aaaacagtat aaaatcaagt caattgtaga aattttcaca taaattcatc
     1741 tgaaattctg taaaaaatgc cttaatttta atgaggtaaa tcgagtttac acagtctaaa
     1801 aattacttat atttataaat ttatcttatt aattaaggaa ctttcttaaa catcttcaaa
     1861 aataaagtat tttagtctat aatctttaat attacacaaa caataaagga gaagaataga
     1921 gtatgcttaa aaagtttaat aaaatactag tggctaatag aggcgaaata gctataagaa
     1981 tatttagagc ctgttcagaa ttggggataa aaagtgttgg tatatacagt aaggaagata
     2041 agtatggatt gtttagaaca aaagcggatg aatcatattt gataggagaa ggtaaagggc
     2101 caatagatgc atatttagat atggatggaa taatagacct agcaaagaga aaaaaagttg
     2161 atgctataca tccaggatat ggttttctag cggagaatgc agaatttgct cgtaaatgtg
     2221 aagagaatgg aattactttt ataggaccat catctaaggt aatgaatatg atgggggata
     2281 agattaactc taaaaaaatc gcaaaagaag ttaatgttca aactatacca ggagtagaaa
     2341 aagcaataag aagtactgaa gaagcaaagg aagttgcaaa caaaatagga tatcctgtta
     2401 tgataaaagc atctaatggc ggtggcggta gaggtatgag aattgtacat cgcgaagaag
     2461 acttagagct tgaatatgaa actgcttgta gtgaatcaag aaaagctttt ggagaagata
     2521 ttatatttat agaaaaatat atagctgatc caaaacatat agaagttcaa atattaggtg
     2581 ataattatgg aaatatagtt cacttatatg aaagagattg ttcagttcaa agaagacatc
     2641 aaaaaataat agaatatgca ccagcttttt cgttagatga taaagtaaga aaagagatat
     2701 gtgaagatgc agttaagtta tcaaaacatg tgggttattc taatgcggga acattagaat
     2761 ttttagtaga tgcaaatggt gggcattatt ttatagagat gaacacaaga gtacaggttg
     2821 aacatactgt aacagagatg gttacaggaa tagatatagt tcaaagtcaa atactaatag
     2881 cccaaggtta cagcttagat agtgaagaaa ttaatataaa atcacaagat gatgtggaaa
     2941 taagaggtta ctctatccaa tgtagaataa ctacagaaga ccctaagaat aaattcatgc
     3001 ctgatacagg taaaattcaa gtttatagaa ctggttcagg atttggtata agactagatg
     3061 gaggaaatgg atttacaggg gcaaacataa gtcctcatta tgatagttta ttggttaaga
     3121 caatatcttg ggatagaaca ttccaaggag caataaataa aactataaga tctataaaag
     3181 aacttagagt tagaggtgtt aaaactaatg taggattcct agttaatgtt ttaaataatc
     3241 ctatattctc aaatggtaaa tgtagtacta agtttataga tgaaaatcca gatttatttg
     3301 aaataacaga aagtaaagac agaggaacaa aattacttca atttatagga gatgtaattg
     3361 taaatgataa tgcttgtaaa gaaaaaccat tatttgatgc attacatgac ccaagaatgg
     3421 ataaggatgg ttcaaaatca gaaggaagta aaatactttt cgataagtta ggtaaatctg
     3481 catatataga aaagataaaa aatgataaga aattactatt aacagataca acaatgagag
     3541 atgcacatca atcattatta gcaacaagaa taagaacata tgacttatta aaagctgcta
     3601 agccaacaga aaaatatcaa aaggatttat tctcacttga aatgtgggga ggagctactt
     3661 atgatgttgc atatagattt ttaaaagaat ctccttggcg tagattacaa aagctaagag
     3721 aagaaatacc aagcataatg ttccaaatgt tgcttagagc atcaaatgga gttggatata
     3781 aaaactatcc agataatgta atagaagaat ttacaaaaga atctgctaga caaggtatag
     3841 atgtatttag aatatttgat tcattaaact gggttgaaaa tatgaaacca tctattaata
     3901 cagctttaga aactggaaaa attgttgaag caactatgtg ttatacaggt gatatactag
     3961 ataagactaa aactaaatac aatctagaat attatattaa aatggcacaa gaattagaaa
     4021 gcttgggagc agatataata gcaataaaag atatgtcagg tcttcttaaa ccatattcag
     4081 cttacacact agttaaagag ttaaagaaaa atgtaaaagc accaatacat ttacacactc
     4141 atgatacaag tggaaatggt gtagcaactt gtttaatggc gtcagaagct ggagtagaca
     4201 taattgatgc tgcattagaa tctatggcag gacttacaag tcagccatca cttaatgcta
     4261 tagtagaggc acttaagaat acagagagag atacaggaat agatttattt ggatatgatg
     4321 agttaggaaa atattataag gatttaagaa aagtttataa taaatttgaa agtgatttaa
     4381 ctaattcttg tgcagaaata tataattttg aaataccagg aggccaatat acaaacttaa
     4441 aacctcaagc tgatagttta ggattagtta atagatttga tgaagttaaa gaaaaatata
     4501 aagaagctaa tgaagttgta ggagatataa taaaagtaac tccatcttca aaagttgtag
     4561 gagacttagc tatctttatg actaaaaata aattagataa agataatata atagaagaag
     4621 gtaaaaattt atcattccca gattctgttg tggattactg taaaggtatg attggtcaac
     4681 cagaaggtgg aataccaaaa gatttacaag aggtagttct aaaaggtgaa gaagctataa
     4741 cagtaagacc aggttcatta ttaccagcag aagattttga tgaaatagct aaatatctaa
     4801 atgaaaagta tgatataaat gctaatataa gaaatgttat aagttatgct ttatatccaa
     4861 aagtatatga agattatatc aaacatctac aacattataa tgatatatct aaacttgaaa
     4921 gtgatgtatt cttctatgga ttaaataaaa atgaagaatg tgaagtagaa atagaagaag
     4981 gtaaggtatt aacaataaga ttagttgaaa ttggtgaagt taaggaaaat ggatttagaa
     5041 ctataggatt tgagttaaat ggaatggtta gagaagtaga aataaaagat aagaatttct
     5101 ctggaaaaat taataatgtt gaaaaagctg atatgaatga ccctcttcaa attggagcaa
     5161 gcataccagg aaaagttata aaaataatgg ttaaagaaga agatgaagta aaagcaaatc
     5221 aaccattaat cgttatagag gctatgaaga tggaaactat aatagttgct aagactgatg
     5281 gagtaattaa gtcaatcaaa gtaaaagaag atgatatggt tgaagataaa caattattaa
     5341 tgataatgaa gtaatttaat taaaataaat taaaaagcac tctgaaatgt ttaataaaaa
     5401 atgttttaga gtgtttttat ttattttaat taaatatttg ttaaaaaaaa gaggacaatt
     5461 acctccagat gtagaaataa agctagtaag gaaaatgtta tctaaaaatt ctaaatatag
     5521 taaatatttt ccataatatt tcttaggaaa taacagcaaa gataaaatac ataaatttaa
     5581 aaggtgggtg cttatatgaa ggtttatgat agtaaaatgt tgagaaatgt agcggtatta
     5641 ggacatagcg gatgtggaaa aacaaatcta atcgaaacta tagcttacac agcaaacact
     5701 aataagatac ctaaattaac agacaaggta aatatgactt atagcatggg attaatacct
     5761 atagagtata atgattataa gtttaactta ttggataccc caggatattt tgactttagc
     5821 ggagacgttg tgtcatctct tagagcaagt gatgcagcaa taatagtaat agatgcaaca
     5881 gcgcctattc aagttggtac agagaaatca ttagaactaa ctgagagtat tcctaagata
     5941 atgtttataa ataaaataga taatgaaaag gcaagataca aagatgcaat agcaatgtta
     6001 agagaaaaat acaataataa gatagttcct atgataagtc ctatatacaa agataaaaac
     6061 tttgtaaagt tacataatgt ttttgaaaac atagatgatt tagaaggtga atttaaggaa
     6121 caagctatga gtgttaaaga agcattaatg gaacttatag cagaaactga tgaccaaata
     6181 ttagataaat attttaatgg agaagaacta actacagaag agatacaaaa aggtattatt
     6241 ataggtatac aaagaggtga tattatacct gtaatatgtg gttcaactat aaataatata
     6301 ggcacaaagg aaatattaga tacaatatca agttatttag aacctatctt tacagaggaa
     6361 agcaaaccat ttaggggatt agtatttaaa actatggttg acccatttgt tggaaagatg
     6421 tcatatataa aaattactga gggtgtactt tctaaggata aagatgtttt taatataaat
     6481 aaaaatgtaa aagaaaaaat agctaatata tatacattga gaaatagtga attagtagaa
     6541 atagaaaaag ctaaagctgg tgatatagta gttataacta aagttaattc tctaaaaaca
     6601 ggagacacta tatctgcaga taaagatgca gaagctttag aaaagataga ttttcctaaa
     6661 ccacaaatat attatgcagt aactcctaag aataaaggtg atgaagaaaa agtagcatct
     6721 gtattaaata aactagtaga agaggaccct acattacatt ggtatagaaa tacagagaca
     6781 aaacaagcac ttttaggagg tcaaggagaa cttcatataa aaacaattaa aaataaaatg
     6841 aaagataagt ttggtgttga tgttgaattg aatgacctta aagttcctta tagagaaact
     6901 ataaaaggaa cagctgatgt acaaggaaaa cataaaaagc aatcaggtgg tcatggtcaa
     6961 tatggagacg taaaaataag atttgaaaga tgtgaatctg attttgaatt tacagaagaa
     7021 atatttggtg gttctgtacc aaaacaatat ataccagcag ttgaaaaagg tttaaaagac
     7081 tctatgcaaa aaggtatatt agcaggatat cctgttacaa acataaaagc tacgctttat
     7141 gatggttcat accatgatgt agactcttct gaaatggcct ttaagatggc agcaagtgca
     7201 gcctttaaaa aaggtatgga agaagcacac ccaatactac tagaacctat aatgaaactt
     7261 aagataactg ttccagaaga atatatggga gatgtcatgg gtgatataaa taagagaaga
     7321 ggaaaaatat ttggaatgga accagatgac aaagggaaac aaataatatt tgcagaagct
     7381 ccacaagcag agacatttaa atatgcaata gatttaagag caatgacaca aggtagagga
     7441 tactttgaaa tggaattaga gagatatgga gaggttccat cacagtttgc agaaaaaata
     7501 attgggttag ctactgcaaa gtaatgaaca aaataaatag aaaaataatt aatatatatt
     7561 attaaaaata tttttagggt tttaactagg tataggttta tgttaggtaa acttatacct
//
LOCUS       DAMJWS010000128         7539 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMJWS010000128 DAMJWS010000000
VERSION     DAMJWS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.133"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            169..1146
                     /locus_tag="PC399_003473"
     CDS             169..1146
                     /locus_tag="PC399_003473"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF2594281.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1139..2107
                     /locus_tag="PC399_003474"
     CDS             1139..2107
                     /locus_tag="PC399_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF2594282.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2291..2881
                     /locus_tag="PC399_003475"
     CDS             2291..2881
                     /locus_tag="PC399_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2594283.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2900..3670
                     /locus_tag="PC399_003476"
     CDS             2900..3670
                     /locus_tag="PC399_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF2594284.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3807..4775
                     /gene="dusB"
                     /locus_tag="PC399_003477"
     CDS             3807..4775
                     /gene="dusB"
                     /locus_tag="PC399_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF2594285.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4884..5363
                     /gene="greA"
                     /locus_tag="PC399_003478"
     CDS             4884..5363
                     /gene="greA"
                     /locus_tag="PC399_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF2594286.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5378..6907
                     /gene="lysS"
                     /locus_tag="PC399_003479"
     CDS             5378..6907
                     /gene="lysS"
                     /locus_tag="PC399_003479"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF2594287.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7216..7410
                     /locus_tag="PC399_003480"
     CDS             7216..7410
                     /locus_tag="PC399_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594288.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         3026 a          756 c         1510 g         2247 t
ORIGIN      
        1 ttaaataaaa tgtaaaatta tataaatcat caatataaaa tataaaacaa ccattataat
       61 aattaaaaag gaaatttaaa attaatagta ttaaaagtat attaataatg tataattatt
      121 tattagaaga aaataagtca tgaaattatg tcatattaga ggtgaaatat gagagataaa
      181 ataatagaga ttatattaaa taataaatct gaatttattt caggagaaga attatcaaaa
      241 caattagggg tgtcacgtgc agctatatgg aaacatatga aagccttaaa agaagaggga
      301 tataatatag aatctgtaaa taaaaaaggg tatcgattag cggaaaatcc aactgattta
      361 ttaagcccac agaatattta ctataaatta gatacagagt ttattggaaa aaatattgaa
      421 catcttgaca ctatagattc aacaaatgaa catgctaaaa ggatagctat aaatagtgta
      481 gatgggactg tgattttaag tgaacatcaa gatatgggga aaggacgatt gggaagaaga
      541 tgggattcaa aacctcatga aggaatctgg atgagtatta tattaaaacc agatatggaa
      601 ccattcaaag ccccatttat aactttaata gcaggagcaa gtgtaacgaa agctttaaac
      661 aatctaggag caaatacatt aataaaatgg ccaaatgatg ttattttaaa tggaaaaaaa
      721 atatgtggta tattaacaga gttatctgct gaaattgaaa gaacaaatta tgttgttctt
      781 ggaattggaa taaatgttaa aataatggat tttgaacaag aaatagctga caaagccaca
      841 tcattgtata aagaagggta tatgttatcc agagtagata ttgttaggaa tgtattgact
      901 gaatttgaaa aattatattt agattatact gtaaataatt ccaaagaaaa aaccttaaaa
      961 atatgtagag attattctgc aattatagat aaaaatatat atgtattaaa aggagataat
     1021 agagaactag tgaaatgttt agacataaat gaagagggaa atttattagt tcaaagaagt
     1081 gatggaagta taaaagaaat tatttcaggt gaagtttcta taagaggaga aaaaggatat
     1141 gtataataat attttggata tagaaggctt aaaggttggg caggttgagg atagaaatgg
     1201 tcttactggg tgcacagtag taatatgtga agaaggagct acttgcggtg ttgatgtaag
     1261 gggctctgct ccaggtacta gagaaaccga tttattagac cctgtaaata tggttcaaaa
     1321 agttcatgca gtagttttgg ctggaggttc tgcatatggt cttgaatcca cttgtggtgt
     1381 aagtagatac ttagaagaaa aaggaattgg atttgatgta ggtgtaggta aagtgcctat
     1441 agttgtagga gcagttttat ttgatttagg agtagggagc tttaaatgta gaccagattt
     1501 aaagatgggt tatagagctt gtgaagttgc aaatactgat attttaaatc aaggaaatta
     1561 tggagcaggt tgtggagcca ctgttggaaa gataagaggt caggaatatg ctatgaaagg
     1621 cggaataggt agctattcta taaaattaga taatggtcta attgtatctg cattaattgc
     1681 tgtaaatgct cttggagatg tttatgaaaa tggtaaggtt atagctggtg ctttaaatga
     1741 taaaaagaca gaaacaattg atacatataa attaatgaaa caaggtgaaa gtaaaggtgg
     1801 atttagtgta gataatacta caattgggat aattgcaaca aatgctaagt taaccaaagc
     1861 tggatgtaaa aagatatctc aaatgggaca tgatggatat gctaagtcta tttttcctat
     1921 ccatacacct catgatggag atacaatatt cacaatatca acaggagaaa tagaagcaga
     1981 tataacatta gtaggttcat tggctgtaga ggttgtggaa aaaagtataa taaatgcagt
     2041 aaaaaaagcg gatagtgtag aaaatataat ggcatataaa gatatagtaa aaaaacgaga
     2101 aatatgatga aattagcata tttttcttgt caaattgttg acaatggcaa ctcatcttga
     2161 taaaattatt cttgttagag cgggcatcct acaatggtag cttgactcat agttataaga
     2221 aaaaaacgaa aaaaattaaa aaattaagta gtccggcatc ttaatgagct ggaacggagg
     2281 tgtatttttt atgatgaaca caagtgcatc agtaagaaaa gtaaatgtaa gaaagatgac
     2341 tataatagga gtactatcag ctatttctat tatgctatct atgactccat tgggatttat
     2401 tccagttgga cctacaaaag caactataat gcacatacca gttattattg gagctattat
     2461 ggaaggacca atagttgggg cagcaatagg atttatattt gggatatcaa gtctcttaaa
     2521 tgctataaca aacccaacag taacatcttt tgtttttata aatccattag tttcaatatt
     2581 accaagagtt atgataggtg tattagctta ttatgtatat cagttaataa ttaaggctac
     2641 aaataaggta tacatatcag gtttaattac aggggcaata ggttctctgt taaatacagc
     2701 tggagtttta ggaatgatat atgtcttata tgctgacaag tatcttcatg caatgggtaa
     2761 aagtggttca gcaggaaaag taataatggc tcttgccgca gcaaatgggg tgccagaagc
     2821 aatagtaggg gctttagtag tagctgctgt tgccactgta cttaagaaaa gcaaaaaata
     2881 gaaagaaggt taatgacata tgcttctagt atttgatgtt ggaaatacta atatggtttt
     2941 aggtatatat aaaggtgaca aattagttaa ttactggaga attaaaacag atagagaaaa
     3001 aacgtctgat gaatatggaa tcctaataag taacctattt gattatgata atgtgaatat
     3061 aagtgatatt gatgatgtta taatatcatc tgtagttccg aatgttatgc attctcttga
     3121 aaacttttgt ataaagtact gtaaaaaaca gccattaata gtaggtccag gcataaaaac
     3181 aggtctaaat ataaaatatg ataatccaaa acaagttggg gcagatagaa tagttaatgc
     3241 tgtagcaggg atagaaaagt atggagcacc aagtatactt gttgattttg gaacagcaac
     3301 tacattttgt gctatctctg aaaaaggtga atatttgggt ggaacaatag caccaggaat
     3361 aaaaatatct agtgaggcgt tatttcaaag tgcgtctaaa ttacctagag tagaattagc
     3421 taagccaggt atgactattt gtaagagtac tgtatcagcc atgcaatctg gaataattta
     3481 tggatatgtt ggtttagttg acaaaataat aagtatgatg aagaaagaat tgaattgtga
     3541 tgatgttaag gttatagcta caggtgggtt agctaaactg attgcttcag agacgaaaag
     3601 tatagattat gtagatggtt ttttaacact agaaggattg agaataatat atgaaaaaaa
     3661 ccaagaataa ttaatataca gggaattggg ttatatatcc aattcccttt ttatgataga
     3721 tttctagcta tatgtggtat attataatga ggaaataaga atttaaagtt aaatcacaaa
     3781 ataaaggcaa aaataggaga aaaacaatga aaataggaaa tttagaattg aaaaataaag
     3841 tttttctatc acctatggca ggtgtaacag accttccatt tagattaatt tgtaaagaac
     3901 aaggatgtgg gcttttatat acagaaatga taaatggtaa ggcactttgc tatgatgatg
     3961 aaaatactaa aaaaatgctt aagatagaag aggaagaaca tcctgtagca gttcagatat
     4021 tcggttcaga accagagttt atgggaagag cagcagagat aatgaatgat tattcaaatg
     4081 agattctcga tataaatatg gggtgtccag ctccaaaagt agttaaaaat ggagatggtt
     4141 cagcacttat gaaaaatcct aagcttgcag aagaggtatt gagggcagta gtgaagaact
     4201 caaaaaagcc agtgacttta aagattcgaa aaggctggga tgataatagt gtaaatgctg
     4261 ttgaaatagc aaagatagca gaggattgtg gaataagtgc tttagcaata catggaagaa
     4321 ctagagaaca gttttataca ggcaaagcag actgggatat aattgcagag attaagaaaa
     4381 atttgagtat acctgttata ggaaatggtg atgtatttac aatagaagat tcaataaata
     4441 tgttagacaa aactggttgt gatgctataa tgataggtag gggtgctcaa ggtaaccctt
     4501 ggatatttaa aagaataaac cattatatga atacaggcga gattttgcca gagccaactc
     4561 ttaacgaaaa aataagtact gccattaagc atttaaaact tgcagtagaa gaacatggag
     4621 agtatgtagc tgtaagggaa atgagaaaac acatcgcttg gtatttaaaa ggacttagaa
     4681 actctgcaaa attaagggat gaaattaaca aaatagaaga ctatcaagaa gttgtgtcga
     4741 aattggagta ctatatggaa gactccttga cataaaaata atagtgactt ataatatagc
     4801 gcataaatta aaaaaagatt ataaaatttt taaaatataa ttaggaaaat tcctaagaaa
     4861 tttgtgaagg agttgtattt ataatggaag aaaataaaga gtttttattg acacaagagg
     4921 gatatgataa attagaagaa gaattagaaa acttaaaggt tgtaaagaga aaagaagttg
     4981 cagagagaat aaaagttgct atatcttttg gtgatttatc tgaaaatgct gagtatgatg
     5041 aagctaaaaa ggaacaagca caagttgaag aaagaatttt aaaattagaa aatatggtta
     5101 gaaaagctgt tatcatagat gaaagcaaaa tagaccttaa tgttgttaca ataggatcaa
     5161 tagttaaagt taaggattta gaatttgatg aagaggttga atatactata gttggttcga
     5221 cagaagcaga cccatatgat ggtaaaatat caaacgaatc tcctgtaggg aaagcacttt
     5281 taggtagagc tgcgaaagaa gttgtggaag tgcaagttcc tgatggagtt gctaagtttg
     5341 aaatattaga aataagaaga taatttggag gaaaaaaatg aaaaataatc aacaaagcaa
     5401 tgaagaagcg caaattcaag aagaccttag tgaagtactt caagtaagaa gagataaact
     5461 aaaaaaatta caagaatcag gtagagatcc ttttaaagaa agtagatatg atagaactca
     5521 ttattctatg gatataaaag ataactttga ttctcttgag ggtaaaacta caaaaatagc
     5581 aggacgtata atgagcaaga gaatacaagg gaaagctggc tttatagata ttcaagacca
     5641 agagggaaga atccaatctt atgtaagact agatgctata ggagaggaag aatatagtgt
     5701 attttcaaca tatgatattg gagatatagt tggaattgaa ggagaaatct ttaaaactaa
     5761 aaaaggtgaa atatctgtaa aggctaaatc tgtagtatta ctatgtaaat ctttacaagt
     5821 tttaccagaa aaatatcatg gattaaaaga ccaagaacta agatatagac aaagatatgt
     5881 tgacttaata gtaaatccag aagtaaaaaa tgctttttta attagaacaa aagcattaaa
     5941 agcattaaga gcatacttag atgatagagg atttttagaa gttgagactc caatattaaa
     6001 tactatagca ggtggggcaa atgctagacc atttataact aatcataata ctttacatat
     6061 accaatgtat ttaagaatag cgaatgaatt gtatttaaaa agacttattg ttggtggatt
     6121 tgataaagtt tatgaaatgg gaagaatgtt tagaaatgaa ggtatggatt taaaacataa
     6181 tcctgaatat actgcaatag aattatatca agcatatgca gactatactg atatgatgga
     6241 aataacagaa aatgttattg cacatatggc tgaagttgct acaggaagta tgatagttaa
     6301 ctatcaagga acagaaataa actttacacc accttggaaa agaatgagta tggaagattg
     6361 tgttaaagaa tattctggtg tagatttttc tactataaat acagatgaag aggcactaga
     6421 agttgctaga gaaaaaggta tagagataaa acctggaatg agaagaggag aagtaataaa
     6481 tgctttcttt gaagaatttg gagaagataa attaatccaa ccaacattta taactcatca
     6541 tccagttgaa gtatctccat tatctaagag aaatgttgaa gacccaagaa gaactgatag
     6601 atttgaggca tttgctaata agtgggagtt agctaatgca ttttctgaac ttaatgaccc
     6661 tattgaccaa aaaggaagat ttatagacca attaagaaaa agagaattag gtgatgatga
     6721 agcttttgaa atggatgaag atttcttaaa agcattagag gtaggtctac ctcctacagg
     6781 aggactagga ataggtatag atagagtaat tatgttatta actaactctc catctataag
     6841 agatgtatta ttattcccga ctatgaaacc tattgataac aatccaaata aagaagaaga
     6901 aaactaagaa atattagcat tctactaagt tttaaataaa gtttagctat taaattatca
     6961 ataaatagct aaattttatt taaaatgtca gtgtagaatg ctttttatat gcttcaaaga
     7021 ttcttttaga tatttaattt ataaattacc aacaaacgtt agaaattgat attagttttt
     7081 gttgattgag actttatatt tagaaaatat gattgaaagt aatttattat gaaaatattt
     7141 agaataattt atgagaaggt aaaaagaata atttatgaga atactatata taaaaaatta
     7201 ttgggggaat gtctgatgtt tcaagtaatt aattttttat gtttattttg tgttggattt
     7261 ttggttcttg atgttatgat atatttttgt aaggatgcaa tggatatttt tgagggtaca
     7321 actaatgtta ataaagttaa gagtataaat gtttgggcag atagagtgag agctaattca
     7381 tattcttatg aaaataaaat tgcaaaatag gtaaaattat atatgcatgt attgtttgta
     7441 atatcaaggg ttatatctat tagttttaat tatatttatt aaaaaaaaca aaagttataa
     7501 ccttttttaa aataaaaaac aaaaaaagta ttgacgaaa
//
LOCUS       DAMJWS010000129         7189 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMJWS010000129 DAMJWS010000000
VERSION     DAMJWS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.134"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            74..415
                     /locus_tag="PC399_003481"
     CDS             74..415
                     /locus_tag="PC399_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2594289.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            566..979
                     /locus_tag="PC399_003482"
     CDS             566..979
                     /locus_tag="PC399_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2594290.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDHIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1181..1822
                     /locus_tag="PC399_003483"
     CDS             1181..1822
                     /locus_tag="PC399_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2594291.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1846..3099
                     /locus_tag="PC399_003484"
     CDS             1846..3099
                     /locus_tag="PC399_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2594292.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3145..3795
                     /locus_tag="PC399_003485"
     CDS             3145..3795
                     /locus_tag="PC399_003485"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF2594293.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3846..4442
                     /locus_tag="PC399_003486"
     CDS             3846..4442
                     /locus_tag="PC399_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2594294.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4455..5294
                     /locus_tag="PC399_003487"
     CDS             4455..5294
                     /locus_tag="PC399_003487"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF2594295.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKKVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5539..6693
                     /locus_tag="PC399_003488"
     CDS             5539..6693
                     /locus_tag="PC399_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF2594296.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            6999..7175
                     /locus_tag="PC399_003489"
                     /pseudo
     CDS             6999..7175
                     /locus_tag="PC399_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
BASE COUNT         2989 a          674 c         1257 g         2269 t
ORIGIN      
        1 catatatttc cgatagatta tattgataaa aaacactaaa attaaattaa taaaagtact
       61 tggagggatg gaaatgaaag aggaaaattt tattaatgtt gaatacgagg ataaacgttt
      121 ttcaattaat agtaaagaag ttttgtacat agaaaagcaa gagtatgaag taaagattta
      181 tactgaaagt ggtaattatg aagttggaga aaatgctcct tgtataagaa aactattgaa
      241 agaagaaaat tttttcaaat gcagtaatag ttatattgta aatctagaca agtgtgaatt
      301 ttatctcagc aataattgta tttttgtgaa taataatttt ataagaattt taggttcaga
      361 aagaaaaaag aaattaaaaa agaaattaga aaaattatat gattatgaag attaatattg
      421 ggaggctgta ttttatacag cctctttatt tattgattgt attattaata aattttaaat
      481 tatgataaaa tataaaacaa gtaacttatt tcagaataaa atacttttta aaagaaaggg
      541 acgagatatg ggagattata ataaaatgct tgaattaaag aataacttag gtatcagtga
      601 aagaaagtta aaatatcctt gtatctataa acatttcaaa gggaaatatt atgctacaat
      661 gggtcttagc aaggctattg atcatataga aaacatatgt gaaatatatg gtaaagaaaa
      721 tttaatccaa aacagaaata aatataaatt agttattaga catactgaaa gagaagaaga
      781 tatctatgtt tatagggatt tagatggaaa cttttatcat aaaaaagaag aagatactaa
      841 tgatttagtt ttatacaaga ccttatatga tgatacagga atatttgcaa gacctttgga
      901 tatgttttta gaaaaagtag atacagataa gtatgtcaat tcaattcaag aatacagatt
      961 tgaagaagta tataaataat ataagagaaa tatgtttata aatttagaaa aattctttaa
     1021 cagtacaata atttgcagaa atacaaatga gatttattgt aaataatatg ttagattaga
     1081 ttataaaata gaaacaaagt aaagtattat atgatataat taagttttga gaagtgataa
     1141 tttatattta ataaaaaata ggcatatatt aggaggacat atgggaaata ctttttatat
     1201 agtaagacat ggacaaacgg attggaatat attgggaaaa acacaaggtc atggtaattc
     1261 tgaccttacg cctcaaggta tagaacaggc aaaagaactt tctgaagata ttggcaagta
     1321 ttcaattgat tatattttta gtagcgacct tgggagagca atgcaaacag ctcaaatttt
     1381 aggagataag ctcaatattg aagttcaaaa aactgaggca cttagagaaa tgggattcgg
     1441 agtttgggaa ggccttttaa taaaagaaat tcaaaaagat tattcagata tatatgcaac
     1501 atggagaaat gaaccacact tagtaaatat accagaaggc gaaactctta agattataaa
     1561 ggaaagagta gatgctttta taaaggaact aaatgaaaaa tatgataata aaaatataat
     1621 tcttgttaca cattcaatta cattgagggt tatgttgttg tcatttttag aatcaggtat
     1681 ggaaaatata tatagaatta agcaagataa tactgcttta aatatagtag aatttaaaga
     1741 ttatgggcct gttatagtaa aaatgaatga tacaagtcat ataaaaaatc atgtaaagat
     1801 aaataattct gcactggagt aggaatcaat tataaggaga aatttatgag taaagttata
     1861 gtagttggtg gaggagcttc tggaatgatg gcagctttgt cagcttcaaa aaacaataat
     1921 gaagtaatat tagttgagag aaatggagaa ttaggaagaa aacttagggc aactggtggc
     1981 ggtagatgca attttaccaa taatagagag atagaagatt tttttgataa agttgtcagt
     2041 aataaaaaat ttctctatag tagtttctat acatttacaa ataaagacct tatttcgtat
     2101 tttgaaagta gaaatttgga atataagatt gaagaagaaa atgaccacaa agtatacact
     2161 aaaaatgata aatctataga agtaatagaa gtattaaata aagatttact caatcacaat
     2221 gtaaagataa tgtataataa gaaagttata gatattataa ctgaagaaat tgctctaaaa
     2281 gatgattcta ataaggacaa gtcaaagtat ctaataaaag ggattatttt agataatgga
     2341 gataaaatac taggagacaa ggttattata tcaactggag gagtgagtta ttcaaagacg
     2401 ggttcagatg gttcaatgta taagattctt aaaaagcatg gacatacatt aaacaaacta
     2461 tatcctgctt tagttccact tacaatagag gaaaaatgga ttaaagattt acaaggaata
     2521 tccatgaaga atgtggaaat atcttgtaag attaagaaaa ggaaaatatc aaaatctgga
     2581 gacatgctat ttgcccattt tgggataaca ggaccttgtg ttcttataat gtcttcttat
     2641 attaacaaga ttattgaaaa ggaaaaggta gaattaaata tagatttctt accaaattta
     2701 agtactgatg aaatctctag tataatcaga gcatttccaa ataaaaatgt tctaaataat
     2761 cttaagcaaa tactacctca aaatttctta aaagagattt tttctctatt atctttagtt
     2821 gataaaaagg caagtgattt gtctaaggca gatgaaatta gaattattga gtatataaaa
     2881 aatatgaagc taacttgtaa tgggactaca ggtataaaca ctggtatggt tacttctggt
     2941 ggtatttcag ttaaagaaat taattcttct actatggaat ctaaattagt taagaactta
     3001 ttttttacag gtgaagtaat tgatatagat gcagaaactg gtggatataa tttacaaata
     3061 gcattttcaa ctggatatct tgcgggtata agtgtataaa tataattttt taataattaa
     3121 atatgaataa atagaggtgt atatatgggt aatttagtta tagctgtaga tggtccagca
     3181 ggagctggaa aaagtacaat agccaagata gtagcaaaaa agctaaatat aaattatata
     3241 gatacaggtg ctatgtacag agcagttact tataagtgtt taaaaagtgg tattgatgtc
     3301 aataatgaaa aagaagttat tcaaatagct gaaaattcgg atatagattt taaagataac
     3361 aatatatatt tggacaaaga agttataaat gaagaaataa gaacaataga agtaagcaat
     3421 aatgtttcta atgtagcaaa aataaaagaa gtaagacagt tgatggttga agttcaacga
     3481 aaaataggaa tgaaaaattc agtaatacta gatggtagag atataggttc atatgttttc
     3541 ccagatgcag attacaaatt ctttttagta gctactccag aagaaagagg aaatagacgc
     3601 tataaagaac tttgtaataa aggatataat actactttag aagaagtaat tgaggatata
     3661 ataagacgag atgaaattga ttcaaataga gaatttgcac cattggtaaa agctaatgat
     3721 gcactagaaa tagatactac aggaaaaact atagaagaag ttgtagaaga agttgtatct
     3781 aaaataaatc tttagttttt tatgcatcat aatgaaaata aatgtagtgt tgaagggaga
     3841 taaacgtgaa tttttataga tttgtaataa atatttttaa gggtttttct aaaatatttt
     3901 ttaaatatga agttataggt gcagagaata ttccagatag aggaaatata gtaatagcat
     3961 caaatcataa atcgaatctt gaccctatat tcttggctgc tgccatagaa aatagagaaa
     4021 tagctgcaat tgcaaagaaa gaattgttta aagtaaaacc attgggattt atattaaaaa
     4081 aattgcatgt tatgcctata aatagagaaa aaccagatgt atctacaata aaaactattt
     4141 taaggtcagt tagagacggt tatgtattag gaatatttcc agagggtact agaataaaag
     4201 gagattcttt tggtaaggct aaggcaggtc tttctgtatt tacaataaaa agtaaatcta
     4261 aagttgttcc agtatcaata atatctaaat ataaactttt tagtaaagta attgtatata
     4321 ttggagaacc aatatcattt gaagaacatt ttaaggaaaa attgagtaat gatgaccatg
     4381 aaaggatatc acaagagatt ttagaagtga taaaacaaaa ttactttaaa tattcaaaat
     4441 aagggggtaa agttatgaac ataaaaatag caaaaaatgc tggcttttgt tttggagtaa
     4501 aaagagcaat gaaaatggca tgggatgaag ttgaaaaaaa tgattctggc atatatgctt
     4561 taggtcctct tattcataac aagcaagcag tagcaaaata tgaagaaaaa ggtcttaaga
     4621 ctgtgaatga aattgataca ataccaaatc atgaaaatat gataataagg tctcatggag
     4681 ttcctgaaaa tatatataag gaagccaaag ataaaaaatt aaaaattgtg gatactacat
     4741 gtccatttgt aaagaaaata catacagtag taagtgaata tcataataaa ggctatgaaa
     4801 taatagttat tggagatatg aagcatccag aagttatagg tataaatgga tggtgtgaaa
     4861 attcagctat aataataaag accttagaac aaatggaaaa tatggaattt gataactcca
     4921 aaagatattg tcttgttgca cagactacaa taaatccaga gttgtatata agtatagtta
     4981 ataaactatc agataaatta gaagaaatag tatttaatga tacaatttgt tcagctacaa
     5041 aaacaagaca agaatcagct aaagagttag ctaaaaaagt tgattgtatg atagttatag
     5101 gaggtaaaca cagttcaaat actcaaaagt tagtaaaggt ttgtgaagac ttagtaccaa
     5161 cttttgcaat agagactaaa gatgaattgg atgtaaatac gcttaaaaaa tataaaaatt
     5221 tgggtattac agcaggggca tctacaccta attggataat agaagaagtt gtaacattcc
     5281 ttgaaaattt ataatttgat aggtaataaa aataggtgga cattgttata atttatcata
     5341 atatccacct ttgatttcat attacatgtg gtaaatagat aacctaatat caaaaagtgt
     5401 gtatcttaag atgaaaaact aagagtataa gatgcgcact ttttaatatt agaaattcaa
     5461 aagaaatatt gatattgatt atcaatagaa aatagaatat aatataattg ttgcaaaatt
     5521 agtctaggag gtaaattaat ggaaatgttg atatccgctt ctgaagaggc gtttttacat
     5581 gtaggttcta tgattggatt ttttattttg ttatttggat atattaatta taaaactagt
     5641 ggaaatttta ctaatatcat atcaaaaaac agaaaatttc aacccttaat aggtgcttta
     5701 attggagcta taccaggttg tggcggaagt ttggcaataa tgccattgta tataaatgga
     5761 aaattaagct ttggagcaat aattgcaagt ttaatagcta gtatgggaga tgcagcattt
     5821 gtacttattt catcaaatat taaaatgtat ttttttgtca ctatagttag tacaattaca
     5881 ggaataataa ctggacaatt agtggattac tttaaactag aagaaaaatt agggttaaaa
     5941 aaccgtagaa aatcagataa atatgatata agtaaaaata accctaaaga aaataaagaa
     6001 caacataatc atgatgaaat aattttagac actctagcta aatctcatgg aaatacaaat
     6061 agacttgcat ttataataac acatggaaag ggatataaaa tttatatagg aattatagta
     6121 atcggtttta tatttatgtc acttgctcac tctggactta atcttcctat aattgagaag
     6181 ttacattcat tggaagaagt aatagcagtg attggaatat tattttcaat aatttatatg
     6241 tggtgtttta aaaaggtatt taaaaattct aatcaaaaag aggaagaaaa taaaaagatt
     6301 tctttgcgag aaatgcttat ccattctgtt ggagaaatat catttgtaat tacatggatt
     6361 tttattgcat atcttattta cgatttgata atattaattt taggtggaga tgaatactta
     6421 gttaaattag tattatcaac aggtgtaata agtgtattta taggtgcagg tcttggactt
     6481 attccaggtt gtgggataca aattgtgctt atgtcatttt atttgaaggg aactatacca
     6541 cttggtgcag tgatagccaa ctctatttct caagatggag atgcactttt cccactttta
     6601 gcaatggata aaaaatcttc tttatgggct atgattatta ctactatacc agcaatactt
     6661 gtaggtatga tagtttatgc atttttaggt taaatataat tatgaataaa gaagtattaa
     6721 aaaaataata aagcacataa atttatttat aagaatagtg aagattaaat ataaaaataa
     6781 aaaattcaat atgaattaaa ttattgctta aatattaatg agaacatggg agataatatt
     6841 attcctcatg ttctctctat ttaattaaag gaattatgga acaaatttca agtgatactt
     6901 tctatcaaaa ctcatatata atatatatta ataaattaaa ctataatacg taatacagtc
     6961 agtatattga tataaaaaac ttaggaggag ttgtaaaaat gggtaattta aaaagacagt
     7021 gtgatgtttc aagtggaaga gaaaaagctg acattgtact aaaaaatggg acaatcataa
     7081 atgtatttac agaagagtta ataacaggag atgtagcaat agtaggagac actatcgttg
     7141 gaattggcga ttacaagggc aatgtagaaa ttgattaaaa aatagctac
//
LOCUS       DAMJWS010000130         6577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMJWS010000130 DAMJWS010000000
VERSION     DAMJWS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.135"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            364..1638
                     /locus_tag="PC399_003490"
     CDS             364..1638
                     /locus_tag="PC399_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2594297.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1845..2162)
                     /gene="trxA"
                     /locus_tag="PC399_003491"
     CDS             complement(1845..2162)
                     /gene="trxA"
                     /locus_tag="PC399_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2594298.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2429..3181
                     /locus_tag="PC399_003492"
     CDS             2429..3181
                     /locus_tag="PC399_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF2594299.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3168..4049
                     /locus_tag="PC399_003493"
     CDS             3168..4049
                     /locus_tag="PC399_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2594300.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTILSGEIMY"
     gene            complement(4275..5630)
                     /locus_tag="PC399_003494"
     CDS             complement(4275..5630)
                     /locus_tag="PC399_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2594301.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2529 a          942 c          830 g         2276 t
ORIGIN      
        1 attatgttct aatcaatttc tttactattt ttacaacttt attagtcata ttcataatta
       61 tttctaatta gtgtttaaca ttttatgtaa aatttataaa aaaatgtata taaattaaaa
      121 taagtgacta tactaaaaat atgatacgga taaacaataa gttcaaccta tctcccccaa
      181 aatcaagtga gctacagttt cccccaactg tagctcattt tcgtgtaatt taataaacat
      241 ctcaacaaga attaattaac aaaacccctt ataaaaacat aacatatatt aaacaagctc
      301 ttgattaaaa cttgagcttt tttaataaaa aacagaatag tttattagat aaggggtttt
      361 tttatgcaat tcatggatgt atctaaatac attgaaggtg caaaccttaa agttaaaact
      421 aaaaatggtg aaactgttaa aagaatatat tttaacaact ctgccactcc attagtttta
      481 aaaaacgtag tggataactt aaactgtgag attccttggc ttacttatat taatgctcca
      541 ggaataatct cagaaaaaaa tactttaaaa tatgaaaatg tacgctctac tatcctcaaa
      601 cttattgatg gagacgaaga aaaggattct gttatttatg tcaaaaatgc tacagaagga
      661 ataaacttct tagcaaaatt ttttaaagat gaaaatccag ataaatttgt tattaccaca
      721 gcaatggagc atatggctaa ttacctgcct tttaaagtta attttcctac aaaagttgtt
      781 ggaattacag aaaaaggaga acttgattta tgtcaattag agaatatact taaaaattat
      841 gctggtaatg tatctctcgt tacagttaca ggagctagta atgtcacagg tataaccact
      901 cctatatatg aaatagcaag aatagctcat aagtatggtg caaagatatt ggtggatatt
      961 gtgcaagtaa ttcagcataa acctttttca atgatgccac atgaatgtga tgagcatata
     1021 gattttgttg tttttagtgc tcataaatgt tattctccac taaatggagg agctctggta
     1081 ggacctaaaa aattctttga aaaatttaat cctgtactat atggctcagg ttctaccaaa
     1141 tttgtaaatg ataacaaaat actatatgca aacgtacccc aacgttttga agcaggatac
     1201 ccagagtatt ttggaactct tgcaatggga aaagcactga acactttaaa taaaattggt
     1261 ctaaacagaa tttcaaaata cgaaagagaa ttatttttat atgctaaaga agaaatgagt
     1321 aaaataccta atataataat atatggagat aaatcaaata atgtaattat cccaaatata
     1381 tcttttaata taagtaatat gtattacaaa gatgtagcaa aatatttagt tgataacttt
     1441 ggaatagaaa ctggagctgg agtagtaggt gcagatattt atgtacaaaa attattaggt
     1501 ataaatccaa aagaggcata cgtcagattt atgagggaaa atcctgtagg tcttgttagg
     1561 ataagcttag gaatgtacaa tacttttgat gaggtggata gctttataca tgctattaaa
     1621 actttggctt ttaaataaga tttttctatt cttttttatc aataaataga gagtagtttt
     1681 gttttcacaa aaaaacaaat tatatcaaca gtcaatttca ttagcaatta gacactcatt
     1741 tgtaactata caaactattt tacactatac aaacaaaaaa ctcattacaa tgagtttttt
     1801 tgtttatata tgagatttat tttaattact tcaatttact ttaattacat atatgattca
     1861 attttgtttt ttaaattctc cttaggcata aaacctatta atctatctac aggttttcca
     1921 tccttaaata ttagcatagt tggtactgta gatacttcaa atttctgagc taattctata
     1981 ctttggtcta tgtccacctt caagaagttt gctttttcaa ccatctcatt tcctaaagct
     2041 tcatatattg gtgatagcat cttacaaggt ccacaccaag tcgcaaagaa atctacaact
     2101 actaaaccat ctttattttc tacttcattt ataaattcat tattatttat tatttttacc
     2161 ataatataca tctcctttat tatcttcttg atattaatat actacgccct atataacagt
     2221 tcaattaggc acttttttgt aaccgcctat ttttaataga tttaatatta tgataaatag
     2281 atttataaga ttaaatattt atgagactaa atatttctaa tatattttat ttttttctta
     2341 atatagttga cattttttcc cttaaattat aaaatcaaat taataactac ataggtagtt
     2401 actaatcttt tatcaaagga gtttctatat ggataatata attaatgaat tacaaaatat
     2461 aggatttact aaatacgaat ctcaaatata catatcatta ttaaaggaaa gccctttaac
     2521 aggatatgaa ataagtaaat tatcaggtgt accacaatca aaagtctatg aaaatataac
     2581 aaagctatta aacaataata taattatcaa tgttggaact gaccctatta agtacatacc
     2641 aataaaccct aatgaattat taaaaaataa gaaaaaagaa tttgacaatt ctatggataa
     2701 tctaaagaat agcttacaga tattaaataa aagtaaaagt gtagaatacg tattaaacat
     2761 aaaaggagat aaaaatatat taaaaaaagc aatagaaatg ataaaaaatg caaaacaaga
     2821 aattataatt tcttgccctt atgaagaaat tcttgaatta aaaaaagaac tcctaattgc
     2881 ttacaaaaaa aatattaata ttgaaatact acttttagaa aacaaaagtc tgaaaggtct
     2941 tgataatatt catattcatg gaggtaataa aactacactt gaaaataaaa tgctttacaa
     3001 aggaattatt ttgattgtag ataatgatga aatattgaca ggaaacttat cagaaggcag
     3061 tgaggccatt tcaatttgga caaaaaactc taacatttta tatattggag ttcaatacat
     3121 aaaacatgaa atttatattt cagaacaaat aaaaggaggt tataaaaatg aacaaaatta
     3181 agattaaaca agttgatgct tttacaacta taccttttgg tggaaatcct gctggtgtcg
     3241 ttactgatgc ctctaacatt tctgatgaaa ttaagcagaa tgtggctaaa gaaatgaatt
     3301 tatcagaaac tgcatttgtt tcagagtcaa atattgcaga ttttaaagta caatttttca
     3361 caccaggttt agaagttgat ttatgtggtc atgcaacaat aggtactttc catgcacttt
     3421 tggaagaagg gaagcttaat actaataaga gtatatttta tcaagaaaca aaagctggtg
     3481 tattatctgt agagcttaag aaaataaata atgaaaatat atttatgatg gaacagaata
     3541 cacctgaatt tgaaaattta gataattgca gaaaagaact tgcacaaatg ataggtgtgc
     3601 atgaagatag acttcttgaa tatcctatta taaaggtaaa tactggactt tggtggcttg
     3661 tatttggtct aaaatcttta gatgatttaa aaaatatctc tccagattta aataaaatca
     3721 aaaaatttag tggtgataat aatataattg gtataactcc attttgtata gaaactatag
     3781 ataaaaagtg tgactaccat ttaagatcta tagcaccata tgttggagtt acagaagacc
     3841 ctgtatgtgg aactgggaac gcttgcgtat catcttatat agtacatcac aatattttag
     3901 ataaaactag ttttattggt gagcaaggta actttataaa cagacctgga aaggtatatg
     3961 taaatataac tagagaaaac aatgacataa cttctgtaaa aattggagga agtgcttata
     4021 ctattttaag tggtgaaatt atgtattaat cccataccta ataaaaacaa ataaaaatac
     4081 tagctaacta aagctagtat ttttatttgt tatttttatt taataatcat actctaacaa
     4141 atattataat tatatatatc ataatattgt atttagaagt aaaattttat ttttattatt
     4201 aattagttat aaatcaatct aatacaacat aattacagta gattaaccta taaaacataa
     4261 taattgctta ctaattattt atcatcctca acaagtctac ttaatgtctt atcagataag
     4321 aataataata tcgctgcaac aaatagtatt actggaatta tagtatatac tttaatcata
     4381 tcaaatttaa gagtaaatgc atatatatat ccgtatcctt taccagctat aaaagtagca
     4441 aatgtccata ctcccataag aacacctaga agcttcttag gtgcatactt acttacaaaa
     4501 gagttaccaa gtggtgagaa taacatttca ccaagactta aaagaatacc aaacatgatt
     4561 atccatagta tactagcttt agcagtttca ggagctccaa cccctcttga aaattctgca
     4621 ccaacaagca ttaagaatgc aagacctaag aatattaatc caagacctgt ctttttaaat
     4681 aaactcatat ctccttgtgg tctactagca agtttaaacc ataatgctcc aagaactggt
     4741 cctaatacta tacatacaag tgaatttaat gagtcaaacc aagcaagagg aacatcaaat
     4801 cctccaacat tcatgtttac aaatgctgca ccataatcat aaactgcaag ataagttaga
     4861 taccagaata cccagaaaat tacagagaag aatgatataa gtataatagc ccaaattctc
     4921 ttcttatcca tgcttgtaag aggagactta tctttttctt caattttttc agcaactaca
     4981 ccttctttaa aaggtctctt tcctgcattc ccaaggaatc tccatccata tatgaaccat
     5041 attgcaccta ttacacaaag tacagctgct ataaagaaac attgagaaaa tccaagtaca
     5101 ccattttttg caaatgtttt aaggtaaaga ataccaactg ctatagtacc aataaaagaa
     5161 cctatattaa caaaagaata ctgtactgaa aatgctgtat caagttcttc ttgactatca
     5221 aaaagctgtc catttaccgc agatacgtta cctttaaaga atgcagtacc aattgaaact
     5281 aagattatca ttgcatttac catagaaact gtagttgcaa tagaaccaag atagtatcct
     5341 actcccatga taagcatacc tataggtata gtatatctag ctcctatcca tctatctgat
     5401 atatatcccc caattatagg tgcaaggtat gtgaatgcaa caaggttggc ttgcattata
     5461 gcagcttgtc ctttatctat tccaagacca ccatgaacta tagctgttgt aaggaatata
     5521 tatattaagt actttgaagc ataatatgct gctctttcaa atgagaaagt tgttgagcag
     5581 atataaaatc ccataggaaa tttacccttc ttttttactg ttgagctcat aattttaaat
     5641 cctttcttaa ttgtattttt attttttacg ttttgtccaa attctttctt ttatgaaaga
     5701 tataatatta ttattttgta aaacaatttg taattattat taagataatt gcgcatttat
     5761 ttgtagagta catattctca tggaataaaa ttaatacact tcttaatcat aaatttaaat
     5821 tatataaatt cactattttc agatatagtt ataaattaat atattctcat ctaataccat
     5881 gaaaaaacct tcgccatatg caattcttat tttttacgtg atagtatttt acaaactaaa
     5941 atcccccttt tgtagcaact tatgtaatta cattttagtg cttttttaaa ggagtctcga
     6001 ataaagcaac atatattttg ttcaaataat gtttttattt attttttaaa tttatatatt
     6061 acttcactca accaattctc aacttaaata actaaactac tatctattca cagattaggc
     6121 ttatttctta cacattagtt tataataaac actgtgaatc taaacataaa aactgttatt
     6181 ttaaagtaat tacccatata ttcgctttga tactatttat tgaggttata ctctttatgt
     6241 ataatatatt atttataaaa tacctcaaaa tatattattc ataaaatacc tcaaactgaa
     6301 aactttttca cgaatattta tttttcacct cccctttttt atttcaccat gtaaatcgta
     6361 ccataaatat gcacttatat gcaagaaaaa aatctattaa tcgacagtaa ataattagaa
     6421 aaattcaaat tatatttatc aagtatatta tataaaatat ttcctatgaa aatataaatt
     6481 aaattaatgt tcagaattgt ttttagatat gaaatcttga tttttttatt actttactta
     6541 tttgctcaaa tatatttttg tatgcagtat ataataa
//
LOCUS       DAMJWS010000131         6504 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMJWS010000131 DAMJWS010000000
VERSION     DAMJWS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.136"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(537..944)
                     /locus_tag="PC399_003495"
     CDS             complement(537..944)
                     /locus_tag="PC399_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594302.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1418..2302)
                     /locus_tag="PC399_003496"
     CDS             complement(1418..2302)
                     /locus_tag="PC399_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2594303.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2593..3069)
                     /locus_tag="PC399_003497"
     CDS             complement(2593..3069)
                     /locus_tag="PC399_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF2594304.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3335..4510
                     /locus_tag="PC399_003498"
     CDS             3335..4510
                     /locus_tag="PC399_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF2594305.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4591..5394)
                     /gene="proC"
                     /locus_tag="PC399_003499"
     CDS             complement(4591..5394)
                     /gene="proC"
                     /locus_tag="PC399_003499"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2594306.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5434..6018)
                     /locus_tag="PC399_003500"
     CDS             complement(5434..6018)
                     /locus_tag="PC399_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594307.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2141 a         1104 c          734 g         2525 t
ORIGIN      
        1 ttaacaaatg taattattta aaacaattat ttagaaggtt ttatatttaa ttttaaataa
       61 catatttaag aaccgtaagt cttaactttt atcattttga ggttgtcgtt cacataagtt
      121 cgctactacc tatgcagttc tcttatgaac ttcttacatt ttcatgtaag cacagactat
      181 atcttatcct tcagcattat ccgttaaggt ctacccactt ccactaccaa tagcttgtag
      241 tgtacttccc tcaagaggaa tagtcgttga agtttctcct gttcggagtt tacctgctga
      301 ttgcccattt taaagtactt aggatttaac cttataccat ctaaccaatt ttttctactt
      361 tcgtaacttt cacacttagg tttatttcat ccttatgttt tagtttgatt agctttaggg
      421 gtttccagca attcaagtag tattggatag gttctatcct atctctacat ataggtttcc
      481 ctatatgctg actattttag ctactaactc atgactaaag tcacgagtgt gtgtagctat
      541 tttttaatca atattttagt aaattttacg gtaagtaaaa acctctctcc ttcaaatcct
      601 atagcaaata tacttacaag ttgctttgag aaaataactc ctaatatagc tacaactatg
      661 catataccaa ctattatatt taaaacattg tttaaaaact ttagtgcttg cttttcacct
      721 tgtttactac ttatatcttg atacattgga ataaaagtag taactatagc agtacctatt
      781 gctgcaaata taatatttgg tatattcata gctgttaaat aactctctgt atataagcct
      841 gtaccataaa ttgatgataa tactaattct ctacccattc caagaatctt agagattata
      901 gtaactatca tcaaataaaa agttgcttta gctaccttgc tcattttatc actccacttc
      961 taacatacct ctttttaatc aagtaaaatt tagctcatta gatataattc tatcaactaa
     1021 tttgccttgc ttagccctat caaaatataa ttaacaccta tttcttaata tatcacaaac
     1081 tacattattc ttcatatttt tttatttctc caataagttt tacatttttc ctatataaat
     1141 tgtatagctt caattatatt caaagaaatg gagttgctac ccgataactt taaaagtaaa
     1201 aaacagattt tattatcttt ttaaactata ttctaacata ttttgtccat attgagaaat
     1261 tttactaaac tattatgtac tttattaaat aaataaaata acagcacacc tataaaatac
     1321 aagtgtgctg ttcataaaca gattttataa aaaaataatt cctaagtaga gattaacatg
     1381 ctttctcata ctctcatata taatgtgtct aaaaatatta ataaaaatgt tttaattgtt
     1441 ttaacttact tggatgtctt aattttctta ttgctttagc ttctatctgt cttattcgtt
     1501 ctctggtaac tccaaatacc tttccaatct gttctagtgt tttagggtca tcatcatcaa
     1561 ttccaaatct cattcttaaa acttgctgtt cttgctctcc aagacctgta agtagttctt
     1621 ctatcttttc ttttaaatta tgctgttcaa cttcgtgtat tacaacatct ttaaaatcag
     1681 catctgatgg tataaattca actaaactac tatcctcatc ttcttttaat ggagtatcca
     1741 aagatacaac atttttatct cttcttaata attccaacac tttgtcaact tctaagccac
     1801 atgcatctgc aatctcatct atagttggct ctcttaataa tacatttaaa agttcttgtt
     1861 tctttttctt tacaaaattt attctctgat gaagatgtat aggtatccta atagtatttt
     1921 cgcaatcgtc gatatatctt gttatacttt gttttatcca ccaagttgca taagtgctaa
     1981 atttgtatcc ttttttataa tcgtactttt ctacagcttt tataagtcct atatttcctg
     2041 cttgaattaa atccaacata tcaatactat ctcttttata tctctttgct atgctcacta
     2101 ccaacctata atttgaattt ataaattttt cttttgctac tgaagatgag ttgattattt
     2161 ccttagccag ctctacctct tctcctgctg acaacatttt gtattcctct atttccttta
     2221 aatacatttt catcgcattc gatacatttg atgaacccac acaaatataa ttttcataat
     2281 tattttcttt tttagtatcc ataatcaaac cccttctatt catagaattt gctaaaacaa
     2341 aaataaataa tttaaaccta cataatctac aattacataa tatcccattc tttaaatgaa
     2401 ttgttttgta tattatgtat acaaatttat aaaaactata cctataatat tattctactt
     2461 ctaacagcaa tatcctcctt atatatataa tttattttat tatatgttgt tatattctta
     2521 gttttttgaa tgatatatgt ctaaaaaaag actgtttact aaacagtctt tttttatata
     2581 tatgctcaat atttaatttt catgatgatg attacatcca catccacaat tttcatcatg
     2641 gtgatgatgt tcttctaaat cttttacctg ttgttccatt tcatgaaact cttttaccat
     2701 tatatcataa gttggaagag ctgcctcagt atcgtattct tcccctctcc aatcagcgta
     2761 catcatagca tctccattag ttaaaaccaa tctacctgac atagttatat tatccacttc
     2821 ttctaaaaat tctatttgtt tttgtaaaac ttctccttct aactccacat cttcatctaa
     2881 acattgcact atcattacag gtgcataata atcttcttca tctttaacat ttacaacaat
     2941 atctaaaatt ccttctgcac cttcttcaaa ttctgccaac tcaacatttg tatcaatcat
     3001 atcttgaggt actaataaat cttctattaa atattttatt actctatctt ttactaattc
     3061 ttttgacatt tatttttctc cttccgattc tataacatta tcataatcat ttctatatta
     3121 tagtatacta cattaattgt tttttcaatc tcttacaaaa attatttttt attatcttcc
     3181 ttatattccc ttttttatta caaagttgta atcgcattga tttaaaaaat agtttatatt
     3241 tagtataata gaaatgtagc acatattttt attcaatata ttaataaaaa aatatattga
     3301 aaatgtaatg tatgttaaat ctaggaggaa tattatgaag tttaaaaaac aaattagttc
     3361 tattgttgtg tctactatgc ttgtacttgg gagtatgaac ctttcttatg cagacggtac
     3421 aaatgtagtt acacttggag ctaacttatc agagtctcaa aagcagcaaa tgcttaatta
     3481 ctttggagta aaaaaagacc aagtcttagt tttagatgta acaaatgcag aagaaagaca
     3541 gtatttacaa ggtgttgcaa cagagggtca acttggaaaa gttactatct cttgctcata
     3601 cgttgaacct actaaaaaag gaaatggaat taatgttaaa acagcaaact taacttgggt
     3661 aactagttca atgattgctt caacccttac aacagctggt cttgaagatg caaatgttat
     3721 tgctgctgct ccatatcctg taagtggtac tggagcattg acaggaatca tgaaagcatt
     3781 tgaggatgct tcaggaaaga aacttgatga aactaagaaa gaaattgctt ctgaagaatt
     3841 ggtagttaca ggtgacttag gagatgatat agggcaagaa aaagcaacag gtgtaatgaa
     3901 tgatataaaa acagaaattg taaaaaatgg tacaaaagat accaatcaaa tagcaaatac
     3961 aataaataat gttgtaaata attacaatat tactattaca ccagaacaaa aagaacaaat
     4021 taaaggtgta atgaaaaaaa tagctgacca aaattacgat tacaatagca tgaaaaatac
     4081 actagataat gtatcagata atgtaaatga acaattaaag aaattaggtg aaagtgttaa
     4141 gggttctggt atactagata ctattggtgg atggtttagt ggaataggag actggttctc
     4201 tggtttattc tcaggcggag ataaaaaaga tttgggaatc ttaaataata ctaatgatga
     4261 gtctcttggc tctgatgctg taataaattc tacagaaggt gttcaaataa atccacaaaa
     4321 taatactaat tctaacaatg atagtgatac aaatagctca aaaaatgata cttcaagtaa
     4381 taatgaaaat aacgatagtt ctcaaacaaa tgaaaacaac acaacaaata attcagaaaa
     4441 tcaatcaaat aataatgact cagttggttt ctttgaaaaa ataaaaaatt ggctttcagg
     4501 cttattttag aaattaaaca aataaaaaga attgcctaaa atggaaattt tctataaaag
     4561 gcaattctct ttatttacaa taaattttta ttactcactc atttttttag atttttctat
     4621 acatgcacat attgctttta tcacagaagc tctaaaacct tcttcttcaa gtactttaac
     4681 tgcttctata gtagttccac caggtgaaca taccatatct tttaattcac caggatgttt
     4741 tcctgtctca agtaccattt ttgcagaacc cataacagcc tgtgatgcaa acttatatgc
     4801 ttgtggtctt ggcattcctg caattactgc tgcatctgct atagcttcta taaataaaaa
     4861 tacatatgct ggtgcagaac cacttgcacc aattacagct tctataagat actctggtac
     4921 tacctcagta tttccaaaag aattaaatac ttctgtaact gcttctaagt cttctttgtt
     4981 tatattttta tttatagaaa gagaagacat ggcttcattt acaagagctg gagtatttgg
     5041 catagttcta actatttttt tatcttctcc tataatagac tctatatctt ttatagattt
     5101 tcctgctgct attgtaacta taattttatt gtcatctata aagtctttta tctcttccaa
     5161 aatatcatca tatatatctg gtttaacagc tacaattact atatcagagg ttctagttac
     5221 ttccttcgag tcttttgtag tatttattcc aaaatttgca tctattctat caagagttcc
     5281 ttgactatat gctgatgcta taaccatatt aggttcaact aattttgaat ttactattcc
     5341 ccctatcata gcgcttgcca tatttcctgc acctatgaat ccaatttttt tcactttagt
     5401 acctccttat tgactaatag taatcgttaa aatttaagct tctttaactg catcagactt
     5461 ttcaaatgga tttggtatac ttactaccaa aattttcact accttattag tatagttgac
     5521 ccaattatga ttgttctttt cactattata gtgtattgtg tctcctggat acatttctat
     5581 ttgttcatct cccaaaaaca ctgtaagagt accctctaat acatacacaa attcctcacc
     5641 ttcatgtacg taacaacaaa tttcctcact actcttactt ggaagaattt caattaatct
     5701 tggtagcatt gttttttcct ttaaactaga tgataaatga taatgtataa atgttgagtt
     5761 ttctacatca aatacttctt tttcataact tcttaataca gctcttttat gctcttttgg
     5821 cttcataaaa aagtaagtta attctacatc tagtacactt gctatttttc ctaaggaatc
     5881 tgttgccaca cttgttaatc ctctttcaag ttgtgataaa aaaccaattg acaagtttgt
     5941 tttttcactc atatccttca gcgtcatttg cttttgagtt ctgagttgct ttatttttgc
     6001 tcctatgtcc ttattcaaaa ccatcacccc taaataattt taattttttc ataatataaa
     6061 tacaaaaata taaatatagg tactgaacct atctttattt catttaaaaa aatttttata
     6121 agtattacta taatttatca atcttttttt gtctacattt ttacgattat ttacgattta
     6181 tttttcacat atgtatattt aatcttgcca actaaagata cattttttat aagcatttac
     6241 tctttatata ataaaactta agttgttcat gagtcttctt ctggtttaat tattaaaatt
     6301 tcaaattata ttattatttc tacacattat tacatccttt agtacaatta taacataatt
     6361 ttacaattta atgatatcta ttcaatgtct ttttttactc tacaacatat tttattttag
     6421 agtttaatac tatatatatt ttttatatac agccttgtca ttttttagat tgtcaagagt
     6481 tattgaagta ttttatgtag attt
//
LOCUS       DAMJWS010000132         6298 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMJWS010000132 DAMJWS010000000
VERSION     DAMJWS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.137"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            233..1177
                     /locus_tag="PC399_003501"
     CDS             233..1177
                     /locus_tag="PC399_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF2594308.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1199..1825
                     /locus_tag="PC399_003502"
     CDS             1199..1825
                     /locus_tag="PC399_003502"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF2594309.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1859..2908
                     /gene="hisC"
                     /locus_tag="PC399_003503"
     CDS             1859..2908
                     /gene="hisC"
                     /locus_tag="PC399_003503"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2594310.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2911..3489
                     /gene="hisB"
                     /locus_tag="PC399_003504"
     CDS             2911..3489
                     /gene="hisB"
                     /locus_tag="PC399_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF2594311.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3491..4096
                     /gene="hisH"
                     /locus_tag="PC399_003505"
     CDS             3491..4096
                     /gene="hisH"
                     /locus_tag="PC399_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF2594312.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4093..4815
                     /gene="hisA"
                     /locus_tag="PC399_003506"
     CDS             4093..4815
                     /gene="hisA"
                     /locus_tag="PC399_003506"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF2594313.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4797..5555
                     /gene="hisF"
                     /locus_tag="PC399_003507"
     CDS             4797..5555
                     /gene="hisF"
                     /locus_tag="PC399_003507"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF2594314.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5583..6251
                     /locus_tag="PC399_003508"
     CDS             5583..6251
                     /locus_tag="PC399_003508"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF2594315.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2573 a          539 c         1244 g         1942 t
ORIGIN      
        1 tagtaaaatt aaatacaaaa ttaaatcatc accatcatca tagattaaag ggattgtaat
       61 atgaaattaa ttcatagaca caagccctgt ttttagtgta attggtcttg tatctatgat
      121 gggaagtaat tttaagagaa aattatagcc atataggtat aaagcctatg tggctttttt
      181 attgttttaa atttgcaaat ataagagtaa atttaatgaa gagggtgaga atgtgaaatt
      241 tttgaggatt gaagatgagg ttatttattc taaaaaaaga tataaattaa aaagggaaat
      301 agataaactt tttttggatg aagaatattt tcaaatagaa ccacaattat ttgaagaata
      361 tgatgaattt acaactataa ataataaaat tccagaagaa tctatggtaa aggttgtaaa
      421 tggaaaagta atggtactta gagctgatat aactacaagt ataataaaaa gccttgtacc
      481 aagatgggaa gatggtttaa agcttaagct tttttataat tcatcaatat ataaaaataa
      541 aaatacagtt ggaataaaag agataagaca aataggttgt gagtatctag gagaagcctc
      601 tgtagaagca gatagagaag tagtgaaatt agctcttaag atacttgaaa aatacaataa
      661 taattttatt ctagaggttg ggagtagcaa gtatattcat ggtttgttag aagaattaaa
      721 tttaaataag aattgtgaaa atcaaattaa aaatttacta tatacgaaga acactcatga
      781 actaaaagta tatattgaag aattaaaaat taagcgtgaa gtaaaagagt tattatcaaa
      841 tattttaagt ttacaaggga atttatataa tgttattgaa aaatcaaaca agttctattg
      901 taataataag atgaagcaag ctcttgaaga attaaagcaa gtaaataact tgattgagga
      961 atgtaatttt ttagataagg ctagatttga tttatcaatg attactatgc ttgactatta
     1021 tgaaggaatt atgtttagag gatactaccc aaactcatat aaagaaattc ttagtggtgg
     1081 aagatatgat tctttaacta aagagtatgg aaaagaaatt ccagccattg gatttacact
     1141 aagtgttgat gagttaatga agtatgtata taagtaaagt ttagaaggag ggattgcttt
     1201 ggattatttg actatagcac tagcaaaagg acgaattgaa ggggaatcat ttaaaaaatt
     1261 taaaaaaatg ggtctaggag acagtataga cacagatacg agaaaactaa tttttaaaga
     1321 tgaagaaaat aaaattatat atatacatgt aaagccatca gatgtagtta catatgttga
     1381 aaaaggagtc gctgacctcg gtatagcagg taaagatact attttagaga atgagacaga
     1441 tgtatatgaa atatatgatt taggttttgg aaaatgtaaa tttgctgtag caggattaaa
     1501 gggagatagc atatatagag aagatgaata tttaaaagtg gcaacaaagt atcccaatat
     1561 agcaaaaaag tattttaaag agaaaggtca aaaaattgaa ataataaaat taaatggttc
     1621 tgtagaacta gctccaatag tagggttgtc agatgtaatt gtagatatag ttgagactgg
     1681 caatacactt aaagcaaatg gtcttgaaat attagaagac atatgtaata taagtgctag
     1741 aattatatca aatagagcta gctatagatt taaatatgaa caaattcaga atataattcg
     1801 tttatttgaa gaacttgata attaaaatat aattgagctt tataaggagg gatttaagtt
     1861 gagagaaaaa gaaagcataa gagagttaaa gggatatgaa ccaaatcacg taaactgtaa
     1921 agtaaaactt gatgcaaatg aaggaagtaa gaggttgttt aaatacctta ttaaagaaat
     1981 ttcagatagt gatatagatt taaatttata tcctgaagat tcttatagtg atttgaaaga
     2041 atctataata gattacataa atatttctgg agtaaataag aaaaatcttc tagtaggaaa
     2101 cggttcaagt gaaataattg atttaataat acatactttt gtagataaag atgaagtaat
     2161 attgagtttc tctccaagtt ttagtatgta ttctatatat agtcagataa atgggtctaa
     2221 atttataggt gttgaaagtg atgaaaattt agttataaat atagataatg tgattgaaaa
     2281 agtaaaggag aataatccta aaatagtaat tgtatgtaat cctaataatc caactggaac
     2341 aatattaaag agggaagaga taataaagct tttagattca actaatagtc tagtggtttt
     2401 agatgaagca tatatggatt ttggagaaga aagcatgtta agtgatgttt ttaagtatga
     2461 taatctaata gttcttagaa ctttatcaaa ggcatttgga ttggctggaa taagaacagg
     2521 atatatgtta tctaatagta gtttaataaa ttctgttgag aaagtaagac ctccatataa
     2581 tttaaattca ttatcagact ttattgctac aagagcactt agaaataaag atgtggtaaa
     2641 ggcttatata aaggaagtaa aagaggaaag agaagtttta tataaagaaa tgattggtat
     2701 gggaattaaa gcatataaat cacaagcaaa ttttatatta ttttattctg aaatcgaaaa
     2761 tttatcacaa aaacttattg atagaggtgt tttaataaga aaatttggtg gaaagttaga
     2821 aaattattac agagttacaa ttggtgataa agaagaaaat tctatgtttg ttggagctat
     2881 aagagatatt ttgaaaaagg agaaataggt atgagaattt ggaaggtaga aagaaatact
     2941 cttgaaacac agattttagt agaattaaat attgatggtt ctggtaaagc tgaaatagat
     3001 actggaatag gatttttaga ccatatgctt accttaatgt catttcatgg caagttcgat
     3061 ttaaaagtga tttgtaaggg tgatacatat gtagatgacc accatagtgt agaagatata
     3121 ggtatagcta taggtgaagc atttaaaaat gctctaggtg acaaaaaagg aatacgaaga
     3181 tattctaata tctatatacc tatggatgaa tctctatcaa tggtagcaat agatataagc
     3241 aatagacctt atcttgtatt taatgctaaa tttgataccc aaatgattgg tagtatgagt
     3301 actcaatgct ttaaagaatt ttttagagct tttgtaaatg aatcgagagt aacattacat
     3361 atcaatctct tatatgggga aaatgaccat cataagatag aaagtatttt taaagcattt
     3421 gcaagagctt taaaagaggg tagtgaaata gtttctaatg agatagcatc ttcaaagggg
     3481 gttttgtaga atgaatataa tagtagacta tggtcttgga aatatagatt ctgtgtctag
     3541 gggttttaga aaagctggaa tagaaactaa aatttctagt gatatagatg aaataaaaca
     3601 ggcagattca cttatacttc caggtgttgg agcttttaga gattctataa gtgccttaga
     3661 taaactagga ttgataccaa taataaagga gcatgtttct aagggtaaat ttatgatagg
     3721 tatatgtcta ggaatgcaat tactttatga aaaaagttat gagtatggag aatatgaagg
     3781 attaggactt ataaaaggaa gtattgataa actggatata agtttaaaag tgccacatat
     3841 gggatggaat aatctaaaat ttaataaggc aaatgatgat atacttaaat atataaatga
     3901 agatgattat gtatacttcg ttcactcata ttatgcaaat tcatcaaatg aagaactaat
     3961 tgcattttca gaatatgaaa agaaaatccc tgctattgtt agaaaaggca atgtatatgg
     4021 tatacaattt catcctgaaa aaagtggaga agtagggtta aatatattaa gagcatatgg
     4081 ggagatgata aaatgataat atttccagca atagatataa aagataataa gtgtgtaaga
     4141 cttacacaag gagaatttga caaagtaaat gtatattatg ataatccatt agaagtagca
     4201 tataaatgga aaaatgaagg agcagaatat atacatatag tagatttaaa tggtgctaga
     4261 agtgaatttg gtgtaaatac aaaaataatt gaggacatag caaacaatat agatatacct
     4321 attcaagttg gcggtggagt tagggataag gaaaaagtca aaagtttaat aaatgctggt
     4381 gttactagag taatattagg tagtatagcg attgaaaatt tgaatttagt tgaagaattg
     4441 gtcaatgaat ataaagagaa aattgtagtt tctatagatg ctaaagatgg aaaagtagca
     4501 gttaggggat gggaagttgt aagcaatgta gactcattaa cattatgcaa acaacttgaa
     4561 aagataggtg tacaaactat tgtttataca gatatatcaa aagatgggat gttgcaaggt
     4621 ccaaattttg acatatatga aagaatagca aaggaaacat cactaaatgt tattgcttct
     4681 ggaggcgtaa cttctataga agacgtaaaa agactaaaag ctatgaactt atatggtgca
     4741 ataataggta aagcgcttta tgataaaaag atagacttta aggaggcaca acaattatgc
     4801 ttactaggag aataatacct tgtctagatg tgagaaatgg aagagtcgta aagggaaaaa
     4861 aatttaaaga tatagtagat gttgatagtc cagaagtact tggaaaattt tatagtgatt
     4921 gtggtgcaga tgaacttgta ttctatgata taactgcatc aaatgaagag agaaagacat
     4981 ctttagaatt tgtgacaaaa gttgcagaaa atataaatat accattttgt gttggtggag
     5041 gagtaaataa attagaagat ttcactgata ttttaagaaa aggtgctgat aaagtttcta
     5101 taaattcatc agcagtcaaa aatcctgaat taataaggga agcttcatta aaatttggag
     5161 cacaatgtgt tgttttatcg atagatgcaa agaaaaatga agagggttca tggagcgtat
     5221 acgtaaaagg tggaagagaa aaaactaatc tggatgcaat tgaatgggct gttaaaggtg
     5281 tagaacttgg agcaggagaa atcgttgtaa atagtatgga tgaagatgga atgaaaaatg
     5341 gatatgatat agaattatta tcaaagataa cttctttagt aaatgtacct gtcatagcat
     5401 ctggaggcgc tggaaaaaag gaagattttt atgaggcggt taataaatcc aatgtagatg
     5461 gaattttagc agcatctgtt tttcattttg gcgagataaa aataaacgat ttaaagaaat
     5521 atttaaaaga catgggtgta gaagtaagat tataagttaa atactaaaag gagattagaa
     5581 atatggataa taagtgtaac aatgtataca gtgatgaagt agagaaattt ataagaagta
     5641 taaagtttga tgataaaggg ttagtccctg ttgttgttca agaggtagtt agcaaagatg
     5701 tactgatgtt agcatatatg aataaagatg ctataaagaa aaccttaaaa gataaagtag
     5761 cttgctattt tagtagaagt agacaagaac tttgggttaa aggtgagaca tctggtaata
     5821 ctcaaaaagt agttaagatg tcttatgatt gtgatgtaga tactatacta ctttttgttg
     5881 aacaaactgg tgtagcttgt catacaggca attattcttg tttttacaga gacttgtttg
     5941 atgatagttc aaaaatggag cttgaagtac aaacaaatat attgaaagaa ttatatgatt
     6001 taatcaatga aagaaaaaat aatccagttg aaggttctta tactaattat ctttttgaaa
     6061 aaggaataga taagattctt aaaaaagtag gtgaagagag tagtgaggta attatagcat
     6121 caaaaaatac cgataaaagt gaacttattt acgaaattag cgatttagta tatcatacat
     6181 tggttttaat gatagaaaaa ggtgttgaaa ttgatgaaat aaaaaaagaa ttacttaaaa
     6241 gaagaaaata gaataagata gattaatggg taataagaaa atataccact tttaataa
//
LOCUS       DAMJWS010000133         6231 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMJWS010000133 DAMJWS010000000
VERSION     DAMJWS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.138"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            180..1202
                     /locus_tag="PC399_003509"
     CDS             180..1202
                     /locus_tag="PC399_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2594316.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1262..2269
                     /gene="gap"
                     /locus_tag="PC399_003510"
     CDS             1262..2269
                     /gene="gap"
                     /locus_tag="PC399_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2594317.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2491..3693
                     /locus_tag="PC399_003511"
     CDS             2491..3693
                     /locus_tag="PC399_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF2594318.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3719..4462
                     /locus_tag="PC399_003512"
     CDS             3719..4462
                     /locus_tag="PC399_003512"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF2594319.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4471..6003
                     /locus_tag="PC399_003513"
     CDS             4471..6003
                     /locus_tag="PC399_003513"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF2594320.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         2482 a          721 c         1258 g         1770 t
ORIGIN      
        1 aaaaaatact gtgatttgaa aatggtggag aaatatttta taaaataaaa aaataaaaaa
       61 gtttttgaaa aactattgaa atctatacgc aaatcctata aaataaaaat atcttgtggg
      121 acttaaataa acactaggga cgaaaaaagt cccgaaagga aagcaataag gagtatgtta
      181 tgaagaactt attagaaatt caaaagaaat tgattccaca ggcaattgaa cttatggaaa
      241 gaagatactc tattttaaga caaatatctt taagtgaacc aataggtagg agaacattat
      301 caaatgtatt agagattagt gaaagaataa tcaggtcaga aactgagttt ttaaaagaac
      361 aaggacttat agacgtagct gtatccggga tgactatcac taaagaaggt tctgatttgt
      421 tagataaact taaggacatc atgagtgaca tcatgggatt gtctaagtta caagatagag
      481 tcagagaaaa gcttggaata aaaagggtaa tattagttcc tggaagctgt gatgaaaatg
      541 gaagtttact aaaagatgta actagatatg gatgcgagta tttcttaggt attttaaaag
      601 atggagatat cgtttcaata acaggaggaa gcactatgtt ggagttttca aacgttatta
      661 aaactgataa gagataccca aactctacaa tagttccagc tagaggaagt atgggaactg
      721 atgtagagat gcaatctaat aatatagttg cttcgataag taagaaatta cattctaatt
      781 gcaaactttt acatatacca gatgagttgg atgaaagtgc aatgaaaact cttagccaag
      841 tgccagagat taagacaact ttggagtata ttaaaagaac agacatttta gtttttagta
      901 taggtagagc agatgtcatg gcaaagagaa gaaaacttcc agaagacaag gttgatgaga
      961 tattatctaa aaatgcagtt ggagaagcat ttggatatta tttcaacaaa gatggagaaa
     1021 tagtttacaa actgaacact gttggaattg atttagaaac agttaaacaa gtaaaagaaa
     1081 ctattgcaat ctttgcaggt actgaaaaag tagaagcctt attagctata tcaaatatgc
     1141 ataaaaacat ggttcttgta acagatgaag aaagtgctta taagatattg tcattattat
     1201 aaaagtcaag acctagcaca gagctagaaa acatacattt actttatagg aggtagcgaa
     1261 aatggttaaa gtagctataa atggatttgg aagaatagga agattagcat taagaaaaat
     1321 gatggagcaa caagataagt tcgaagttgt tgcaataaac gacttaacag acgctaaaat
     1381 gttagctcat ttatttaaat atgatacagc tcaaggaaga tttaacggag aaatagaagt
     1441 taaagaaggt gcttttgtag taaacggaaa agaaataaaa gtaacagcag aaagaaatcc
     1501 tgctgaccta ccatgggctg aattaggagt agatatagta ttagaatgta caggattctt
     1561 tacatctaaa gataaagctg aagctcatat acaagcaggg gctaagaaag ttgttatatc
     1621 tgcaccagct actggagatt taaaaacaat agtattcaac acaaacagtg atatattaga
     1681 tggttctgaa acagttatat caggagcttc ttgtactact aactgtttag ctccaatggc
     1741 taaagtatta aatgataaat acggtataga aaaaggatta atgactacta tacatgcata
     1801 cactaatgac caaaatactt tagatggtcc tcatccaaaa ggtgatttaa gaagagctag
     1861 agctgctgct ggaaacatag ttccaaacac tacaggagct gcaaaagcaa taggcttagt
     1921 aataccttca ttaaaaggaa aattagatgg ggcagcacaa agagtaccag ttgtaactgg
     1981 ttcaataact gaattagttt gtacattagg aaaaaatgtt acagttgaag aaataaatgc
     2041 agctatgaaa gaagcttcaa acgaatcttt cggatatact gaagaaatgt tagtatcttc
     2101 tgacataatc ggaataagct atggttcatt atttgacgct actcaaacaa aagttatgga
     2161 agtagatgga aaacaattag ttaaagttgt ttcttggtat gataatgaaa tgtcttatac
     2221 ttctcaatta ataagaactt taggatactt tgcacaatta gcaaaatagt cagttttgag
     2281 aaaatagaaa agtagattga caaagtccga gtttgtagtt taatactacg gactcaggac
     2341 tttttttaag aaaaaatata taaaatacac ctaaataatg ttacatacta tatataaaaa
     2401 tatgataata tgatatattt aatagggtaa aagtatgtac tatattatac ttggcaataa
     2461 aaaattactt tattaaaagg agagatattt atgtcaatgc ttaacaaaaa aactatagaa
     2521 gatattgatg tgtgcggaaa aaaagtatta gtaagatgtg actttaatgt gccattacaa
     2581 gatggtgtaa ttactgatga aaatagattg aacggagctt taccaactat tcaatattta
     2641 atttctaaag gtgcgaaagt tatattatgc tctcacttag gaaagccaaa aggagaagct
     2701 aagccagaat tatctttagc accagtagca aagagattat ctgagatgtt aggaaaagaa
     2761 gttgtatttg cagcagacga taatgttgta ggagaaaatg caaagaaagc aactgaaaaa
     2821 atggaaaacg gagatgtagt attattagaa aatactagat atagaaaaga agaaactaaa
     2881 aacgaagaga atttctctaa agaattagca tcacttgctg aaatatttgt aaatgatgcg
     2941 tttggaactg ctcatagagc acattgctca acagttggtg caggtgagtt cttacaagag
     3001 agagtttgtg gatacttaat tcaaaaagaa ttaaaattct taggagaagc tgttgcaaac
     3061 cctgtaagac catttacagc tatacttgga ggagctaaag tttctgataa attagctgtt
     3121 ataaacgaat tattagaaaa agttgataat ctaataatag gtggaggaat ggcttataca
     3181 ttcttaaaag ctcagggtta tgaagtagga acttctttac ttgaaataga taaagttgaa
     3241 tatgctaaag aaatgatgga aaaagctaag aataagggtg ttaacttatt attaccagta
     3301 gacgttgtta tggcagacca ctttgcacca gatgctactc caatagtaac agaagatgct
     3361 aatgtaaaag aagattacat gggactagat atgggaccta agactatagc aaactttgta
     3421 aaaactataa aagaatcaaa aactgtagta tggaatgggc caatgggagt atttgaattt
     3481 gaaaactttg caaatggtac attatcagta gctagagcta tggcagagtt gactgatgca
     3541 actactgtaa ttggcggtgg agatagtgca gcagcagtta atcaattagg atttggtgac
     3601 aagatgactc acgtatcaac aggtggagga gcatcactag aatttttaga aggtaaagaa
     3661 ttaccaggta ttgcagcatt agataataaa taaactaata tatagaggag attgtaaaat
     3721 gagaaaacct ataattgcag gaaactggaa aatgcataaa acaataaaag aagctttaga
     3781 atttgtaaat gaagttaaag ataaagtaaa ttctgataaa gtagaagctg ttatatgtgc
     3841 accatttact ttattaaagg atttaaaaga agctactaag ggtacaaata taaagatagg
     3901 tgctcaaaat atgcactttg aagaaaaagg agcatttaca ggagaagttt cacctcttat
     3961 gttaaaagaa atagatatgg actatgttgt aataggacat tcagaaagaa gacaatactt
     4021 caatgaaact gatgaaactg taaacaagaa agttttaaaa gcattagaag taggaataga
     4081 cccaatatta tgtgttggag aaactttaga acaaagagaa gctggaaaaa ctaaagatgt
     4141 ttgtaaagtt caagttgaaa aagctttaga aaatgttctt aaagatgatt tagcaaaagt
     4201 agttgttgca tatgagccaa tctgggctat tggaactggt aaaactgcaa ctgctgaaga
     4261 tgctaatgac gttatatctt atataagaga agttataaaa ggattatatg gagaattagc
     4321 taatgaagtt agaatacaat acggtggaag tgttaaacct tcaaatgtag ccgaaataat
     4381 gggtcaaagt gatatagatg gagctttagt aggtggagct agtttagcta gtaatgatta
     4441 cctagacctt gttaatttct aaggagtgaa atgatgaaaa aaccagttgc tttaattatt
     4501 atggacgggt ttggatacaa taaggatgtt aagggaaatg caattgctga gtccaaaact
     4561 cctaatttag atagaataaa aaaagagtat ccaaacacat tgataaatgc tagtggtctt
     4621 gatgttggtc tacctgatgg tcaaatggga aattcagaag ttggacacac taacataggt
     4681 gcaggtagaa tagtatatca agatttaact agaataacaa aatctataaa agatggtgac
     4741 ttttttacta ataaagtttt atgtgaagca atggataatg caaaagaaaa ttctcttcat
     4801 gtaatgggac ttttgtctga tggaggagta cattcacata tagaccatct taaggctata
     4861 atcaaaatgg caaaagataa aggcgtacaa aaggtgtatg ttcacgcatt tactgatggt
     4921 agagatactg accctcaaag tgcattagaa tatgccaaag aagtacaagc tagtatggat
     4981 gaaattggag taggtgaatt tgctacagtt tctggtagat actacgctat ggatagagat
     5041 aagagatggg aaagagtaga acttgcatac aatgccatgg ttagaggtat tggtgaaaaa
     5101 gcaaattcta ttgaggaagc tattcaaaac tcttatgatg atggaaaaaa tgatgagttt
     5161 ataatgccaa cagttattat gaaagatgat aaaccagttg gaagtataaa ggaaaatgat
     5221 tcaattatat tcttcaactt tagaccagat agagctagac aaataactag agctttagtt
     5281 tgtgaagagt ttgatggatt taaaagagaa gatattaaaa acttctttgt atgcttaaca
     5341 gagtacgata taactataga gaatgtgcat atagcatttg gacctcaatc tttagccaac
     5401 actctaggtg agtatctagc taaaaatgga aaaactcaac ttagagctgc tgaaactgaa
     5461 aagtatgcac atgtaacttt cttcttcaat ggtggagtag aagagccaaa taaaggtgaa
     5521 gaaagactat tgataccttc tccaaaggtt gcaacttatg atttaaaacc agaaatgtca
     5581 gcatatgaat tgacagataa agctttagat aaattaggag aagataagtt tgactttata
     5641 gtattaaact ttgcaaatcc tgatatggta ggtcatacag gaagtataga agcggctata
     5701 aaggctgttg aaactgtaga tacttgtgta ggaagattga tagataaaat agtagagtta
     5761 ggtggaagtg caataataac tgctgaccac ggaaatgctg aatatatgtt agacccagaa
     5821 acaggaaaaa ctgtaacggc tcactctatt aatccagtac catttattgt agttggtcaa
     5881 gaatatgaaa gtgcaaaatt attggatgga ggaagattat ctgatatagc accgactgtt
     5941 ttagatatga tgaagttaga aaagccagaa gaaatgactg gtcattcact tatttcaaaa
     6001 tagtcgattc tgataagtag agaataatat ataaactaca atataaattt aaaaataatg
     6061 cagtttaggt ttattttgta aagtttagac tttatgacag aagatttaga ttttgtaagt
     6121 tttggatatt gtaaggtgta gatgaagata tattttataa aatttacaag atagacaatt
     6181 tataaaataa aaaattataa tatagataat aatgtaaata atgatataaa t
//
LOCUS       DAMJWS010000134         6077 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMJWS010000134 DAMJWS010000000
VERSION     DAMJWS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.139"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            190..1002
                     /locus_tag="PC399_003514"
     CDS             190..1002
                     /locus_tag="PC399_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF2594321.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1091..1282)
                     /locus_tag="PC399_003515"
     CDS             complement(1091..1282)
                     /locus_tag="PC399_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594322.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1553..1792
                     /locus_tag="PC399_003516"
     CDS             1553..1792
                     /locus_tag="PC399_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2594323.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            2101..3720
                     /locus_tag="PC399_003517"
     CDS             2101..3720
                     /locus_tag="PC399_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2594324.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDEHFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            4094..4288
                     /locus_tag="PC399_003518"
     CDS             4094..4288
                     /locus_tag="PC399_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2594325.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(4847..5170)
                     /locus_tag="PC399_003519"
     CDS             complement(4847..5170)
                     /locus_tag="PC399_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594326.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(5215..5469)
                     /locus_tag="PC399_003520"
     CDS             complement(5215..5469)
                     /locus_tag="PC399_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594327.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            complement(5733..6014)
                     /locus_tag="PC399_003521"
     CDS             complement(5733..6014)
                     /locus_tag="PC399_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594328.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT         2236 a          763 c          736 g         2342 t
ORIGIN      
        1 ttgaaataat cactttttca agaatgttat aattttttta ttgtattctt tttgctatag
       61 tctatgaata aatcaataga aaatcttcta agttgctttt aaataatgac tttatggttc
      121 aattgtacta aaatagtata tgtaggttac aaataatcta ataaatatta aagtttggag
      181 gtaatagata tggcagattg taattcatgt ccatcaaagg gaaattgtaa tagtcaatca
      241 aattgttcta ttgaaaataa ccctaacaat aaatttggaa aaataattgg tgttatgagt
      301 gggaagggtg gagttggaaa atcaactgta actgctttac ttgctaataa gctaaacaaa
      361 atgggatata aggttggtat attagactca gatataactg gaccaagtat tcctagactt
      421 atgggtgtaa aaaatgtaaa agcttattct gatggctctt atatttaccc tgtagaaaac
      481 tcaaataaca taaaagtaat gtctataaac cttatgatag atgatgaaaa tgaacctgtt
      541 gtttggagag gacctttact tggaggggtt gtaaagcaat tttatactga tgtactttgg
      601 gaagaattag actatttatt aatagatatg cctcctggta ctggagatgt tgccttaaca
      661 gttatgcaat ctattcctat aagtggaata gttatggttt cagtacctca agatttggta
      721 tctatgattg tatcaaaagc tgttaatatg gctaaaaaaa tgaacataaa tgtccttgga
      781 gttattgaaa atatgagtta tatccaatgt cctgattgtt ctaaaaaaat caagttattt
      841 gaaggagaat ctactgaaaa gtttttagat gatttagact tagagctttt aggagaactt
      901 cctatgacta aagaaattat agatattact cataatggag ttacagaaat tagtgatgat
      961 ttagattcaa tattgactaa tgttgttgaa aaaattaaat aaataaaaat tttataattt
     1021 tatatttatc aaaaaaagag gcttgtattt attttataat catacaaata aatataaacc
     1081 tcttttatgt ttatctatgt tttaaataca caaaatattc ttctacttca tctccatttt
     1141 ttacttttac taaatattca ttataatgag cattatatgg ctgtgtcatt ttgcattcta
     1201 ttacattttc tatgtcttct aattctaaaa gttctacatc ctttaaaaat tttctttcta
     1261 tgagttccat taatctttcc ataatatatc ctcacttccc ctataattaa gataaactat
     1321 atatcctctt aatgcagagt ttatctgttt attatattat atccaatata ttacagtttc
     1381 taacatcatt cataaattat tttaacaaat agagattgat tttttaataa atatgccttg
     1441 ataaaatgaa acatagacca actcttagtt ttatgtaaag aaaacttaaa gaaaactata
     1501 aattatattc acaaaaatta ctcagaagat ataacagtaa gtaatatatg taatgaatta
     1561 caacttaata aaagttattt tttctttgtt taagaaaaaa agtggatata cttttactaa
     1621 ttttttaaat aaagttagag ttgaaaaaag caaaaagtat atattaaaaa aagacttgtc
     1681 tattttagac gttgctgtac taattggatt caatagtcaa aattactata gtataatttt
     1741 taaaaagttt aataacttta caccaattga atataaaaat atttataact aaaaaatatc
     1801 attctaaaag gatgttattt ttttacatat tttttacaat tatagttaat tctttgttaa
     1861 attgtttgta cataaaatac taaaaatcta agttcaagta tttaaattgc tatatatcat
     1921 aaattttcta aaattaacaa caacttaaaa atttaacatt ctcttaacgt atacttaaca
     1981 aaacattaat atttttattg acaaaatttg ttattctata ttatggaaac aataaatttt
     2041 agttaataaa gtttataatt cagataaaaa aattattaat ttcttaaggg aggaaatatc
     2101 gtgaatatag taattagcct tatcggtgga cttggacttt ttctttacgg aatgagttta
     2161 atgggagaag gtttacaaaa atcagcagga gacaaattaa aaaaaattat tgaactctta
     2221 acaagtaatg ttgttatggg tgtactggta ggtacagtcg ttacaggtat tatacaaagt
     2281 tcaagtgcta ctactgttat ggttgtagga tttgtaaatg caggtataat gaatctaagt
     2341 caagctattg gtgtaataat gggggcaaat ataggtacta cagttactgc tcaattagtt
     2401 tcatttaatc ttgaaggaat tgcaccaatt gctttaggta taggtatttt attctattta
     2461 tttacatcaa atcaaaaaac aaagcattta tctgagatac ttataggttt tggtatatta
     2521 tttacaggaa tggagtttat gaaagatgca gtagctccac ttgctgaata caaagcattc
     2581 actgatgcac ttttatattt tagtaaaaat cctgtattag gtatattggc aggatttgcc
     2641 ataactggta ttgttcaaag ttcaagtgca tctatgggta tgttaatagc actagcttct
     2701 caaggaatac ttcctctatc atctgcttta cctattcttt atggagataa tataggtact
     2761 tgtgttactt cacttttatc aagtgttggt gcaagtagaa atgctcgacg tgcagctgta
     2821 atgcatttaa gttttaatgt aattggtaca ataatattta tgcttgtttt aaataaacct
     2881 atatcagcca tagttactca ttttgaccca actgatacag ctagacaaat agcaaatgca
     2941 catactttat ttaatcttac aaatgttata atattattac cattttctaa atacatagtt
     3001 aaattagcaa atagacttat acctattaaa gaaactgaat cagaaatagt aaataatact
     3061 aagtacttag atgaaaggat gtttagcact ccatctatag cacttggtaa tactgtacaa
     3121 gaagttgtaa gaatgggtca taaagctaca aactctttgg aacattcaat tgcaggattt
     3181 ttaaataaga gcaatgaaga tattaataaa gcttttgagt cagaaaaagt tgtcaataag
     3241 ttacagaaag atatattgaa ctatttgtta aaactttcta aagaacctct tagagatgat
     3301 gaacacttta gaacagattt gctttttaat actgtaaatg acattgaaag agtctctgac
     3361 catgcagaaa atatagctga attggccatg agtgttaagg aaatgaacat tagtttttct
     3421 gatagtgcga taagagaaat atacgaaata tacaataaga caattactaa ttttaaggat
     3481 gccttaatag ttttagatgt taaagatttt gagttagcaa ataaagtttt agaagttgaa
     3541 aatgaagtaa attatttaga aaaaactttt agaaattcac atatgattag actaaataat
     3601 ggaagttgta caattgatgc tggtgtactt tatcttgact tacttactaa tctagaaaga
     3661 atttctgacc actcaacaaa tatagtaaaa caagtactaa aacttaaaca aaaaatatag
     3721 tgattatttt tatatataat cttaatcaat gagaagtaag aagctataga taatgcttat
     3781 atcatttatg gtttcttatt ttttatgtaa tactcaaatt tttatagtat tctcttatta
     3841 ttaaacaatt tctgcctaga atattccata tatatatgac ttctttcata aaacttgaac
     3901 tgcccataac taaagtcaca agtatacata actatttttt aatcaattat aaccaatatt
     3961 tgttttattt atcttcattt taattcttca ttcaataatg taataagatt attttttatt
     4021 tggatactat tatatcatct aaatatgtta ttatatattt ggatattttc atattaatag
     4081 gagggattct acaatgataa attatatggt agaaattata tatagcgaaa aaataagcga
     4141 agtagaaaac atgttgaaaa ttgaaaatat acaatatgaa tttacttctc gtgatggctt
     4201 tatatttttt ggtttttatg atatgacaga cgcatataga tttgagactt tatgttcaga
     4261 tgaaaaaatt cgatttaaaa gaatataata gataaaaaag tacatatttc taaactattt
     4321 gtttttcaaa tttaggttgt gttttaatgt agtgttgata ttataaacaa ttttaataat
     4381 aaaaaagtag ggacattata ttaatatgct cctacttttt actcatactt attaatatta
     4441 tttatcaaaa ttaccctaca aactggaatt tatcattctt tcataatttt aaactatcat
     4501 atttaaaata tataaaattc cacatgattt ttgtataaac ataatatcaa ccattatcca
     4561 atttaaaaat attttttagt aatttccatt tcattttctc ttaacatatt aattaaattt
     4621 ttatatgtaa atggtgtatt tacatattat ttaatatcca acaattatat aaagaaatgt
     4681 gctctttgcc ttataaagaa ttataaagcc aattcgtttt attatattat ttttctttat
     4741 accttaaaaa ctttaccttc aaattcttat ttatctatat attataaaaa tgttcttaac
     4801 ctcttttacg agattttctt gacatattat cagttagata aaataattaa ataatataat
     4861 cagcaagtat gttttttact cttttaagta aatttccacc tgcaccccaa tcaattttta
     4921 aaattccaat tgcactgcct gttgaagtag ctttaataac tgtttttcct gtggtatttt
     4981 cataaactac tattaatcct gcatcactat tatttcttgc ataatacttc tcaaaagttg
     5041 taattagtat aaatttatca tctcctaaat tataagaatc actaaaaact aaatctgcat
     5101 caagattttc ttttatcatt ttttgtgcct ctaagggtgt aatttttaca gtaaatattt
     5161 ctgttgccat tttttatctc ctcataagaa tttattttag acaaaacata atctctagta
     5221 ttttctgata tatttatcaa acaatatccc tattcctcct aacagagcta atatagaacc
     5281 aaaatttcta tattgagaaa tagattggtc tagataaata ttatattggt cagtatccat
     5341 gattccacca ttttgttgaa taatatgctc tgcactaaat tcacctaaac ctatacaaca
     5401 gaataatact actactccaa ttacaaaagc aaatatacat attgttaatt taaaatcctt
     5461 attttccata gttatccctc tcataagcta aatttatttg attattttta ctagatttta
     5521 tattactctt attatacaat acaacatata tttacatcta tttccaaaat tgtaaccttt
     5581 atagttttat atatttaaat tttttaaaca aaaaatacac atctataaat tataaatgtg
     5641 tattctgtac cattactaat aatatattat acttttttac aaatttctaa ttatttataa
     5701 ctattcaaaa ataaaattca tataaaataa ttctattgta caattgaaac tttaataatg
     5761 tctccatttt ttctctccaa gtgaactact tctccagtta aatcatagtc aaatgcctta
     5821 actaatattt cagtaacttt tttaccatct actacatcat cttttccatt aaataaatcc
     5881 aaggcattat tctcaatcat tctctttgca aaatttacag gtaactttat atgtatttta
     5941 tcgcctgtat gtgataatac tcttattctt aaaaactttt ctaaattgtt tcttggtgtt
     6001 tgttttgatg ccatactatt aaatcactct cctttatttt ttatttaata ataaatttta
     6061 ttcttaaaaa tatcttt
//
LOCUS       DAMJWS010000135         5995 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMJWS010000135 DAMJWS010000000
VERSION     DAMJWS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.140"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            144..431
                     /locus_tag="PC399_003522"
     CDS             144..431
                     /locus_tag="PC399_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594329.1"
                     /translation="MKKILYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFMTIKIFIFLSLPLCFNILFNYLTS"
     gene            complement(970..1521)
                     /locus_tag="PC399_003523"
     CDS             complement(970..1521)
                     /locus_tag="PC399_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594330.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1712..2674
                     /locus_tag="PC399_003524"
     CDS             1712..2674
                     /locus_tag="PC399_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF2594331.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2969..4813
                     /locus_tag="PC399_003525"
     CDS             2969..4813
                     /locus_tag="PC399_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2594332.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5135..5719
                     /locus_tag="PC399_003526"
     CDS             5135..5719
                     /locus_tag="PC399_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2594333.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSLDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2340 a          583 c          928 g         2144 t
ORIGIN      
        1 aaatataagg agatgccctt tgaaaataaa aattaaaaaa tatttaatgc tactacaata
       61 ggaactagaa ctacacttaa taaatataca gaaattagat ttggctcaat ataaaataca
      121 aatatagaat ttaggtgttt tttatgaaaa aaattttata tgctttatat agtttcattg
      181 ttattatagc taattttaga ttaaaagaaa aaaattataa ctttatttta ttagcaatct
      241 tttttatatt gctgttaatt caaaatggag ttttaaaaac aggttatcaa aatagagcta
      301 cattttatca agataattat tttaatggag aggataaaaa tgataagata aaatttatga
      361 ctataaagat ttttatattt ttgtcattac cattatgttt taatatacta ttcaattatt
      421 taacctctta aattatattt agatatagga gtcttatgat aaaagtaagc ttatgtgtta
      481 tttgtagtaa atataaagtg catatagtga taaaacaaaa gtttcatata aatgaagaaa
      541 aaaatataaa ataatttatt taactactta aaaataaact aaaagggata ataaaatagc
      601 ttttagttta tggtaataga ggtatttaaa aagagtgcat aagcactcca attgtgagaa
      661 atacatacac aaatcattat tacaacaatg aaaaatgtac atataagaga cgttttgtat
      721 ttgttttaag tgataatatt ttaatactaa ctaaataatt atcacaatgg taaaaaagaa
      781 aaaggagtat tttgtactcc atttgtcaaa aatataaaac ttttatataa gcttattata
      841 acataattga taaaagtgta aagatatata atgaatttat aaagaaaaat aaaataaaaa
      901 agataaatcc taatcaaagg atttatcttt ttgtatagaa aataattatt ctattattaa
      961 atggcatggc taaactggat aataatttat tattactgct ttcaatgttt tttctccttg
     1021 attatggtat gtatgaattg attcagctga aaaacaaata gagtcatttt ctctaagttg
     1081 ataaatagag tcatttactt ctaattttaa ttgaccttct ataatcataa tatattcctg
     1141 tgatttttca gaatgaccaa cagatgtata actatgacct tcttctaatt ccatttgaaa
     1201 taattcgaag tttctagttg gtgtattggg ataataacaa taaagacgat aatctttatt
     1261 ttctgaaact tgtacatcta tatcagtttt tgatacaata aaagtttcat tttgaggttg
     1321 ttccagtatt gcagtatatg gaacatttag tccacttgca attttccaaa ttgtgtttac
     1381 tgttgggttt gagtctccct tttcaatttg agaaagcatt actttactta cgccagataa
     1441 ttcagcaagt tgacctagac ttaagttacg ttcattacgt aatctattta aatttttagc
     1501 aataacagta ttaatactca tagtagctcc ttaaaatgta aattattgac aaatataatt
     1561 tacgaatgta taatttattt aacattattt ataatttaca aatgtataat ataatgaacg
     1621 ttaaatacac tgtacatccg taatataaat tatactgcat aaagagttgt ttttcaacgg
     1681 atgatagttg agagattaag ggggaattgt aatgcgtgta cttaataatg ttacagaggc
     1741 tataggcaat actcatatgt tacatttatc caaaatagca gatgaatttg gagttcaagg
     1801 taatatttat gcaaagatgg aatacttaaa tccgggattt agtaaaaagg accgtgtagc
     1861 attacaaatg attaccgaag cagaaaatgc aggtttatta gttccaggtc aagcagttgt
     1921 ggaattaaca agtggtaata ctggaacagg actagcaata gcttgtgcat gtaaaggata
     1981 taaatttata gcgtgtatgt caaaaggaaa ttctatggaa agagcaagaa tgatgaaagc
     2041 tttaggtgct gaagtagttc ttgtagacca aatgcctaat tcagttcatg gacaggtatc
     2101 tggtgaagat ttggcacttg tagaaattga ggctcaaaaa atagcaaaag aaagaaatgc
     2161 atttagagca gaccaattta atttagaagc ttgtaatcat gcacatgaaa agtatacagc
     2221 agaagaaatg tgggaacaat tggatggaaa tattgatgta tttgtagatt ttcttggaag
     2281 tggtagtact tttgcaggat gttcaaaaac acttaaaaaa cataattctt ctatcaaatg
     2341 ttatgttgtt gaacctgaaa cagcagcaat ttattcaggt aaagaaataa ctgaccaagg
     2401 gcataaaata caaggtggag gatattctat ggatttgcca ttagtagaca agaaacttat
     2461 tgatggttat attcaagtta cagacgagga ggcaactgat gtagcaagaa aattagctaa
     2521 attagaaggt gtatttgcag gattttcttc aggggcaaat gtatgtgcag caattaagtt
     2581 gttaaagagc agtgaaaaag gaaaaaatat agttttaact cttaatgata gtggtttaaa
     2641 atatttaagt actgatttat atgaagaatt gtaataatag cacaatagtt atgcaatgtt
     2701 ttttaaatta agtatataga tttaaattat aaaaagtatc tcaatgtaaa ttttgaaagt
     2761 tataaaggag atactttttt attttgtaaa tatggagtat cctacttata agtattaagc
     2821 tatatagata ttgatgatta gctatgcaat aaaaaagagt atgtgttttt gcatttatac
     2881 atcataactt ggaataatac atattaatgt tctaaatgta tttaaaatat atgataaaat
     2941 tagaacaaat agatacaagg agataaaaat gcaaaagaga attttaagaa attttatatt
     3001 tacgggtaaa aatattttaa gtaaacttgt ttttataata aaatataatt ttagtacatt
     3061 aattttattt gaattatttc ataaaggatt agccttattt tttatatggc catctattag
     3121 atatatattt gatattttaa taaaaagttt acatatagtc tacctaaata tggataattt
     3181 tataaaagtt ttgaccaatc ccatgtcaat agttctgata atactatcag ttataatact
     3241 agctttttat gcattttttg aatttacctc tgtaattatt tgttttaata aatcgataaa
     3301 atatgaaaaa atagggttat ttgaactctt taaaatatca tttgagaact ctataaaatt
     3361 gttatatcct aaaaatatac tattatttat atttgtgata ttaataattc cattagtaaa
     3421 tacagtttta atttcagggt ttataggaaa aatacaaata ccagagtaca tattagatta
     3481 tataaagtct gatataattt taaatataat ctatatgatt tttcttttaa taatatatat
     3541 ttcggttatt agatggattt ttagcattca tgagataact ttaaatacag aaaattttaa
     3601 aagagcaaga aaagaaagtg ttaatcttat aaagggaaag attataagaa caattattta
     3661 ttcattaata ttatctttag tagtagctat tattggatat gcgatttatc atagtggaat
     3721 tatatttata ggcatatgga ctaaatattg tactgatatg gaatttttga agaatgtatt
     3781 tataaataga tgtattgcat ttgaagaata ttttagattg attttatcaa tattcatttt
     3841 tatattggac attggattta tctcagcaac ttattatcaa tataaaggag taaatatttc
     3901 taaaaataat attaaaaaag agaaaaaatc tatatcaaaa attgttttta atttagtatt
     3961 aatattattg atttcttata tagagtcagt tgcattttct tcacatataa atggtatgtt
     4021 taatacgtca ttttcatttt atacaacagc aattgctcat agagcaggag ctgaaatggc
     4081 tccagagaat actttggcag caatagaaga agcttcacat gctaaagctg attatgcaga
     4141 aatagatgtt caagaaacta aagatggaga acttattgta cttcatgatt caaactttaa
     4201 gagaactaca ggagtagata aaaatgtttg ggatgtaaat tataatgaag taaagacata
     4261 tgatgcggga agcttttatt attatggaaa aggttattat gatgaaaagt ttgttgggga
     4321 aaagatacca acactcaaag aaatgataaa atattctaaa ggtaaggtga aattattaat
     4381 agaaataaaa cttaatggac atgaaaaggg agacgtagta aaaaaagttg ttcagttaat
     4441 taaagaaaat aattttgaaa atcaatgtat agttgcatct atggataaaa ctatattaca
     4501 aaaagtaaaa aaactggatg agaatttact gacatgctat tttatggcaa ttgcatatgg
     4561 tgatttttca aagttaaatt atgttgatat atatggaatt gaatcaactt ttgttaataa
     4621 aacagtagta gaaaagattc ataaaatgaa caaaaaaatc ttcgtttgga ctgttaatag
     4681 tgacagttta attgagaaaa tgcttgacct aaatgtagat agtattatta cagataatcc
     4741 ataccttata gagagtgcaa tttattggaa gaaaaatgga tttattagtc aagtgtcaga
     4801 ttatttattt taaatattct ataattgtat tatccagtaa aagagatatt attaaaatca
     4861 ataatatctc tttttaacat aaataaggtt gttaaattaa ggctattctt ataaaaattg
     4921 tgtttatcag atataaataa ggaataaaag taaaaaaatt gctatgacat tattacaatt
     4981 aaaagagttt taatatgata gagaggtaga ttgtcaactg tatatttaat aaattttatt
     5041 atttattaga ttttatataa gtaaaatata tagatatata aatcttatgc tagaatatat
     5101 atgaaaaaca atatagattt ggagggttaa aagcatgaca gagcgagaaa aaatgttggc
     5161 aggagaactt tacgattgtg gtgatattga gttgttaact caatggcata aagcaaaaga
     5221 cctagtaaga gattataacc aaacagattc cttggatttg gaaaaaaaag aaaaaatatt
     5281 aaatgagtta cttggtggta agggtaaaaa cttatggatt actgcaccgt attatgtaga
     5341 ttatggaaat aatatttatt ttggaaataa ttgtgaagtc aatatgaatt gtacgttttt
     5401 agatgataat aaaattatca ttggggataa tgcactaatc gcaccaaacg tacaaatata
     5461 tactgcattt catccaacta atgctggtga tagatttggt aatgccaaag aagatggttc
     5521 atttgaattt tgtaaaactc aaacagctcc agttactatt ggagataatg tttggattgg
     5581 cgggggtgct attatcatgc caggtgttac tattggagat aatgtagtta taggtgcagg
     5641 aagtattgtt acaaaagata ttccaagtaa tatgattgca tacggaaatc catgccgtat
     5701 tattcgagaa aataaataaa gttaaatttt cttgcaaaat ctcaaaaaac acctttctta
     5761 ctttatacat agggtatttt ctaatacttt cacatccata ttcatttgtt aaatttttgt
     5821 ggaatgtagt ttttatcact tctatcttac atttccacca tttttttata aaagtcattc
     5881 ctttatagat ttatttataa tagtatatat tctatttgat gtttgaataa gcacttattt
     5941 ttaagtaaaa tactaacttc taggttagta ttttactata gtatgatgat taagt
//
LOCUS       DAMJWS010000136         5965 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMJWS010000136 DAMJWS010000000
VERSION     DAMJWS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.141"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            103..669
                     /locus_tag="PC399_003527"
     CDS             103..669
                     /locus_tag="PC399_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2594334.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            709..1290
                     /locus_tag="PC399_003528"
     CDS             709..1290
                     /locus_tag="PC399_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2594335.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1347..2264)
                     /locus_tag="PC399_003529"
     CDS             complement(1347..2264)
                     /locus_tag="PC399_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2594336.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2394..4025
                     /locus_tag="PC399_003530"
     CDS             2394..4025
                     /locus_tag="PC399_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2594337.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFVRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4028..4975
                     /locus_tag="PC399_003531"
     CDS             4028..4975
                     /locus_tag="PC399_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF2594338.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5260..5523)
                     /locus_tag="PC399_003532"
     CDS             complement(5260..5523)
                     /locus_tag="PC399_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF2594339.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5622..5867)
                     /locus_tag="PC399_003533"
     CDS             complement(5622..5867)
                     /locus_tag="PC399_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594340.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
BASE COUNT         2144 a          817 c          981 g         2023 t
ORIGIN      
        1 aaatatagcc aacttagcct aaatacgtat ataatatagc taatatggtt cttatacttg
       61 ataccatcaa taaaactaaa aatataaaaa ggagaactta aaatgagtgt tagtttacaa
      121 aaaggacaaa gagttagctt agtaaaagat aataatcctg taaaaaatct agtagtaggc
      181 ttaggatggg atatgaataa attgggtaaa aaaaattatg acctagatgc atttgcaata
      241 gcattgacta accaagataa aatgaatgtt agaggtgact tggtttattt tggaaatctt
      301 aaacaccctt ctaaagctat tatacacact ggagataact taactggaaa aggtgaaggt
      361 gatgatgaac aaataattgt taatttagaa gacatacctg aatatgtaca taaaatagtt
      421 tttggagttt ctatatacaa agctaaaaaa agagaccagg attttggtca aatgaacaac
      481 tcttttataa gattaattga ttcaaattcc aagcaagaat tatttaaata taatcttcaa
      541 gaagactttt ctggtaaagt aacagttttg gcaggagaaa tttatcgtag aaatgaagaa
      601 tggaaattta atgctttagg aattggtcaa aatgaagagc ttcgtgaatt aattaataca
      661 tataagtaat aattacaata aaaaatttat aatgaggagt tggtttaaat gggtataact
      721 ttagcaaaag gacaaaaagt aagtttaaca aaaagtaacc ctggtctaaa aaaagtaata
      781 gtaggactag gatgggatat taataaatat gatggtggct ttgatttcga tttagatgcc
      841 tctgcatttt taactggaac tgatggaaaa gtaacgaatg atggagactt tatattctat
      901 aataatttaa aacacgcttc tggagctgtt gaatatatgg gtgataatag aacaggtgtt
      961 ggtgatggtg atgatgaaca aataaatgta gacttatcta aaatacctca aaatattgct
     1021 aaaatatcat ttagtgttac tataaacgaa gcaattacaa gaagacaaaa tttcggacaa
     1081 gttgaaaatt catatataag aatatataat gaagaaacta atgaggaact tattaaatat
     1141 gaattgggtg aagattttag catagaaaca gcaatagttg tggcagaact atatagacat
     1201 aatggagaat ggaaatttaa tgctttaggt tctggatttg aggatggact tgcaggattg
     1261 tgtaaaaatt ttggcgttaa tataggctaa tatatctaaa taaaaataag agtatattga
     1321 tatactctta tttttattta gatatattat aaactgttta cacttctact cttctttctc
     1381 gcttgttttt tcccatattc tactgaaata tcaaaaaatg ctgaaatatt ttctgtaaaa
     1441 aacttgcttc tatgatatgc aatatcaaat gtcctatgta aattaatacc ttcaatatca
     1501 caagcccaca aaagatgctt ttttaaataa tcctcaataa gtaattcgga tataactgca
     1561 atgccaaaat tatctacaac tgctcttaat atagcttcag agttataaca tgaccatcta
     1621 atattcattg ggatttttaa ttcttttaat tgcttttcta attgtgctct tgtaccactt
     1681 cctaattcac gcataattaa tggttgattt gaaagttctg aaacttttat agagtctctt
     1741 ttataaaact catgcttgtg tgaacatata agtacaagat tatcattaat aatgcttttt
     1801 gtaacaatat ctgggtgctt aatttttcct tctacaatcg caatatctaa ttcactatta
     1861 agtaattttt ttgcaatcag tcttgtatct tctacaaata cttcaggttc tataagagga
     1921 tttaccttgt ataattctaa cattattgga cttattacac atgtaccaac agttactgtt
     1981 gccccaactc ttatacggat attttttgca caatgcttca tacaatcatt catttcttca
     2041 tttacagcta aaagatgcct cccatatcca agaagttttt tccctgcctc agttaaatat
     2101 aatttttttg aaattctatc aaatagctta acaccatact ctttttctat ttctgaaata
     2161 gcttggctaa ctgatgactg ggtaatatac atatttcttg ctacttcact cattttacca
     2221 cattcagcaa ccttaacaaa gatttcaaga ctacgaattg tcatattatc acaaccttag
     2281 caaacattag taaatgctta tgattttatt aatattttag tattttacaa atatattgtc
     2341 aagaagtata attttattat agagttataa agtagattgg agtgtgatat attgtgaaag
     2401 tattaattat aggtggagtt gctgctggaa caaaaactgc tgcaaaaata aaacgtgaaa
     2461 tgggtgataa ttgctctgtt acaatactaa ataaaggtga tgatatttct tatgcaggtt
     2521 gtggtcttcc atattacgtt ggtaagataa ttgaagataa atcatcttta atcgtaaata
     2581 cacctgaaag tttttctaaa ctaacaggag cagaagttcg ttgtggagtt gaagttacat
     2641 ctattgatag aaatgcaaaa caagtaactt tgaaaacgct ttcaacagga gaaaaagaaa
     2701 atctaagcta tgacaaactt gtaattgcta caggggcaga cccaataaaa ccaccaatac
     2761 ctggaataga tttacctgga gtattctttg tgcgtactcc aaatgatgct ataaaactaa
     2821 gagattctat tgaaaacggt attaagagag ctgttgttat tggtggaggt ttcattggtc
     2881 ttgaaattgc tgaaaatctt tctgcaatgg gtatacgtgt aagtgttgtt gatatggctg
     2941 aacatgttat gcctggattt gatactgatt ttgctgaata cgttgaaaat catatggctg
     3001 ataaaggcat aatgattttt acaggtgacc aggttgtagg tatcgaaggt gaaaataaag
     3061 ttgaaaaact tcgtactaaa aatagagcta ttaaaacaga tttagtggtt atgtcagttg
     3121 gaatccgtcc taacacaggt tttctaaaag acacaggttt agaatttgcc cctaataata
     3181 caattattgc agatgaatat atgcttacaa atgacaaaga tatatacgta gttggagact
     3241 gtgcttttgt aaaaaattct ttaacaaata aacctacatg gtcacctatg gggtcatctg
     3301 caaatcatga gggtagaatt tgcgcacaaa atatatctgg aaaatctaaa acttacaatg
     3361 gtgttcttgg aacaacagta gtaaaattac ctgagctcaa tgcaggaaaa acaggtctaa
     3421 gtaaagaaat agctgaaaaa gaaggttata atgtttgctc tgtaaccata gctactgatg
     3481 ataaagctca ttattatcca ggtgcatcta attttattat aagaatggtt gctgaaaaaa
     3541 atactagaaa attattaggt gttcaagtta tgggacctgg agcggttgat aaaatagtag
     3601 atatcgctgc tactgcaata actttaggcg ctgaccttta ttcattagaa agtatggacc
     3661 ttgcatatgc accacctttt tcaacagcta ttcacccttt tgtagtggca gtaaatgttt
     3721 tacaaaataa acttaatgga gaacttgata gtgttttact tgacgaaatt gaggattttg
     3781 acagttggac taaacttgat gtttcaaaag ctccttctat tcctagttta cgttatattc
     3841 ctgttggtga aattaatgga gaaattgaag gccttgctaa ggatgaaaaa attcttctta
     3901 tatgtgctaa aggtaaaaat agctatatgg cgcaaaatcg cttaagacgc tttggctata
     3961 caaatacgaa agttcttgaa ggtggaatac tattttatcc aaatcttaag gtaatggagg
     4021 agtaaatatg agtaatgtta cagcagaaca aaaaaaggaa gtaaaaggta gaggttttct
     4081 accaagcagg gatggagaac attttgcagc acgtataata actgtaaatg gtgtaataaa
     4141 tgcatcacaa gcaaaaaaaa ttgcagaagc tgctgaaaaa ttcggaaacg gtcagttagc
     4201 atttactact agactaacag tagaattacc tggtgtaaaa tttgaagata ttgaagctct
     4261 aagtgaattt attgctagcg aaaatctaat aacaggtgga actggatcaa gggttcgtcc
     4321 tgttgtggct tgtaaaggta ctgtttgtgt tcatggtcta gctgatacac aggcacttgc
     4381 tgctgaaata cacgaagaat tttacaaaaa ttggtatgat gtaaaactac ctcataagtt
     4441 caaaattggt gttggtggat gccctaataa ctgtattaaa cctgacctaa acgatcttgg
     4501 aattgttggt caacgtgtac ctgactatga ttcagagctt tgtgttggtt gtaaaaaatg
     4561 tgctgttgta gaggtctgtc ctgttaaagc agctaaatta acagataaag gtaaacttga
     4621 aatagatagt aatctatgta ataactgtgg taagtgtata gaaagttgta attttgatag
     4681 tattgaagaa aaagaatctg gttataaagt ctatatagga gggaaatggg gaaaatcagt
     4741 tcgtcctgga acacagattg atagattatt ttcaaaagag gaacttatga ctttaattga
     4801 aaaagcaatt cttttattta gagaacaggg taacacaggt gaacgttttg gaataactat
     4861 cgatagaatc ggtgctgata aatttataga aatgctttta tcagatgaag ttttggaaag
     4921 aaaagaagag atactagagg ctcttttaca ccttacaggt ggagctgtat gctaacaata
     4981 aatattttaa ttgtacagat aacctatgga aaaaatttta gttaagaata attaaaattc
     5041 tatatatgat atatgataaa tgaggctatc tcaaaacaca gatacgcctt tttttcattg
     5101 ttgaaatgat aaagtatatt tgagttcata taaaaaagag tgtctcgtga attataaaat
     5161 tcattttgag gcacccacat tattttataa taattgctta ttaattttat tttaactata
     5221 taaaaagtac aaaaagtaag aatatccaat attctaaaat taattttgaa ttgtattgat
     5281 tgaaaagttt actattccac tatttccatc ttgatttact actagatacc atgttccagt
     5341 attaggaact ctcattctac atggattttt ctttgcaaat ccaccatagt aatcataatt
     5401 tctttgattt ttaaatttca cataatttat atggtctaac aatattacat tggcttgttt
     5461 ctctaaattt acctctacaa tatccccttt ttttaagttc atcttactat aactatattc
     5521 cattgaatta ctcctccagt atcgaataat ctttgttatt tactcatctc aaatatgtaa
     5581 aaattcattt aaaatattac aaatactata taattagact attatccagt tttaacctta
     5641 tttattacaa tccaaaggtt attaaaaata ttattggcaa caacttctcc atcccataca
     5701 actctttctt ctttattttc taataaagca tagtacccag agtcagaact ttcatcttta
     5761 tagactgtaa cataaaactc ttcatcacta tattcaggta tatttttaaa gtcttcagat
     5821 attttacatg ttaatatata accttctaat tcttcatcta taaacaaagc aattcctcca
     5881 atctctatat ttttactaat tataccatat tatgtcataa actttttatt ttattacaaa
     5941 tttctatttt ttctttactt tataa
//
LOCUS       DAMJWS010000137         5957 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMJWS010000137 DAMJWS010000000
VERSION     DAMJWS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.142"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            84..737
                     /locus_tag="PC399_003534"
     CDS             84..737
                     /locus_tag="PC399_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2594341.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            878..2149
                     /gene="brnQ"
                     /locus_tag="PC399_003535"
     CDS             878..2149
                     /gene="brnQ"
                     /locus_tag="PC399_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2594342.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2351..3538
                     /locus_tag="PC399_003536"
     CDS             2351..3538
                     /locus_tag="PC399_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF2594343.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3768..5792)
                     /locus_tag="PC399_003537"
     CDS             complement(3768..5792)
                     /locus_tag="PC399_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2594344.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT         2095 a          707 c          932 g         2223 t
ORIGIN      
        1 tttaatatat caaatatgta tattaagtta atatattaaa aaaaataata tcaaaaataa
       61 tataatttat agtggagggg aaaatgacta aaattattaa agtgcaaaaa cctaaattta
      121 caggagaatt agaaattatt gaaaatcttg aagatatact tgaagatata tttaatgatg
      181 aaaaaatatt taataaaatt atagaaaatg aagttataag tgatttagat tctgtaagag
      241 ttagctttga atcttgtata tttagaaatg tgtcttttga aaattgtaat ttaaaaaaga
      301 ttgatttact tgatgtggta tttgaaaact gcaatttatc aaatacattt tttgatggag
      361 gaagtatata cagagttgaa tttaaaaata gtaaattgat aggagttaga tttgatgatt
      421 gtatacttaa aaacgttttg ttcaaggatg ttttaggtaa gtattctaac ttttcattct
      481 ctaaatttaa gatagtttct atcttagatt caaattttga aaattctgta tttcaagaag
      541 taaaaggtga caatctagta tttgagtata caaatttaaa aaaagcaaac tttaatggaa
      601 ctaaactttc tggcatagat tttactacta ataatattga gggaattgag attggtatag
      661 atgatattag aggagcaatt tttgatgtgt cacaagcaat tgatttaacg aaattgatgg
      721 gaattatcat aaaatgaaaa aaatattcat aaaataaatt gggaaaacgt taacaatgtg
      781 tgaattttta ccagattgac atttttctga aaataaagta agattatctt aatattcaaa
      841 atattttcaa agggggtaat ttagtgtgag taagcagatg agagatattt ttatagttgg
      901 ttttgcttta ttttctatgt tctttggtgc aggaaatctt atgtttcctc catttttagg
      961 gatggaatcc ggcaaagact ggttaattcc tcttattgga tttgttgttg cagatgctgg
     1021 tatagcaatg cttgttatta tagcaacagc aagatgtaag ggaagcatgg atgatgtact
     1081 taggagagct ggtaaagggt tagcaagagt aatgagtgta gctgctctag catgtttagc
     1141 acttcttgta ataccaagaa cttgcgcaac aacatatgaa atgggtatta tgcctatatt
     1201 tggagaccat ggtccagtag ctaaagctgt attttcaata atcttctttg ggctaacttt
     1261 agcatttact attaggtctt ctaaggttat agacataata ggtaagtatt taactccagc
     1321 gttattatta gtattatttg ttttaatagt aaagggtata atttctccag caggacctat
     1381 gagtcctgag catatgatag ataaaaatct atttggtgaa ggtatatcac aaggttatca
     1441 aacaatggac gctctaggtg cagcatctat ggcaactata atgttgatgt ctataatagc
     1501 taaaggatat acatcagata aagaccaaat aggaatgact attaaagctg gttttgtagc
     1561 ctgtgtattt ctagcagtag tatatggtgg tcttacttat ttaggtgcaa cagtttcaac
     1621 tttatatgat actagtatac ctcaagcatc tttattagtt gaaataacta gtatattgtt
     1681 atatggtcaa gtaggaaagg taattttagg tgtaattgtg gcacttgcct gtctgactac
     1741 ttctacaggt ttaacagcat caatatcaac ttattttgaa ggaataagta aggcgagtta
     1801 taaacaactg gttataggta tatgtgtagc tagtatgctt atatcaaatt taggtgttga
     1861 ttctataata gccatatcag ttccaatcct acaaactata tatcctgttt tattagcaat
     1921 agttgttatg gagttggctg gtaaacatat aaagaatgat aatgctttta aaggtgcagc
     1981 atatgttaca ttagtgatta gtttattatc agctataaat ggaatgacag gagctgttcc
     2041 atttatacaa agcttgccac ttgcaggttt gggctttaac tgggtaatac cagctatatt
     2101 gggtggaata gttggtaact ttataaaaag caacaaacaa gtagcataat aatttgtaat
     2161 taatttaatt ttataatata ttaattttta gtactatggg taggtttaat ctaaacctaa
     2221 tctacccata gtacttttta gattggatta taatagcatg tatataattt tttataatat
     2281 aatttttctt tgaagtatga taaaattagt aatagtgata agaatgtaca aatttaggag
     2341 gttttaatta atgcttagaa aaataacagc atttatgcta tccatatgtt taattattgg
     2401 acttacaggt tgtagtttac ttgtaggcaa gaaagataaa gagaaaatta tagatgattt
     2461 taaagttgca gtggtaactc aaccattatc tgaaaataaa gtacaataca atatggtgga
     2521 agagatggca aaagaatatg aggaagagaa taaaattgat aaagacaaag atggtcaaac
     2581 taaagttaaa caaactataa agcatgttgt attgccagag aattttactt ctaatataga
     2641 cagtgctatt aataagatag taaagttggc tgacgataaa gaagttcagg ctattgtagt
     2701 ttcaactgac caagcagggc ttttacctgc tttacaaaag gtaaaggaaa agcgtccaga
     2761 aataataact atttctgccc ctatgggaga tgataaaaac caattaagcc aatttgttga
     2821 tgtgaatcta ggggttagtg cagaggaaag aggaaaagta ttagctgaac gttcaaaaga
     2881 aatgggagca aaagctttta ttcattatgc ctctacagat gatttgaaag atgttaatat
     2941 agccaaaaga ttagaaatga ttaaagagac ttgcaaaaat ataggattgc catttgtaca
     3001 agttaatact ccaaatataa atactgaaga agacaaaaac aaagttaagc agtttttaaa
     3061 tgaagatatt gaaaaacaag ttaaaaaata tggaaaagat ataaatgtat ttggtgtaaa
     3121 tgagtatatg gatgaagtta tattgactaa ggctttagaa ttaaagtata tagttgcaga
     3181 gcaaagcaat ccatcaccaa tacaaacata tcctagtgtt atgggtctaa aaatatctga
     3241 aaaagatgct caaaattatg ataaaataaa cgacatgata agtgaaaagg ctaaggcttt
     3301 tggcatgtca aatagacttg gtggatatcc tatgccaatg gatgcttttc taccatcagt
     3361 agcaatttat cttgctactg aaatggtgaa acaagattta acacaagaag atgtatgtga
     3421 cccagattat ttagaggcgt ttacagaatt aagatttgga atcggttcag aatttactcc
     3481 tttaacagag gtactttata attatcaatc agtaatttta agccaattaa tttattaata
     3541 ttaaggaagt gtctcaaaat gaacttttta gtttatgagg cactcttttt ctattaaatc
     3601 aaatatattt ttatcttttt aacaattaaa aagaagctta tccctatatt gagatagctt
     3661 cgtttttaat ataaataagt tttattattt tataaaaaat ttatagaaaa ataaaaaatt
     3721 ataattatat ataaattttg ataaagtaaa gatgaattac tataggttta aattatatta
     3781 ttttctatta tttttatgcc tttctcagtt attgatgagc cttttcttcc tttactagaa
     3841 attaagtatc cattgcgtgt caaatattct agcatatttc ttacttcgta ttctgtgaca
     3901 ttatattcca taaatttcat taactttgtc ctccccattg gtttcatgtg caatgatttt
     3961 aggatagaaa tgcatatgtt tttgtcatat tggtttaaat cattgaatat ggacatatta
     4021 cttgacatat gattattatt taattttgaa gatatgtttt gaggtaaatc atttattgta
     4081 agataattgg aacccatgta agccatatat tcaatacaat ttctcagttc tctaatattc
     4141 cccatccaag tgtatgaatt taaagcttct aaaagactct tatcgagttt tatgttttta
     4201 tagggaagtt cttttaaaaa tgattctaaa agaagaggta tatcttctaa tctctttcta
     4261 agaggaggta aatctaaagt gaacatactt atcctataaa ataaatccat cctaaattta
     4321 ttttctataa ttaatttctc taaatcttta tttgtggcag ctaatattct cacatcaatt
     4381 ggaataatac ttgtaccacc aatttttcta atttcttttt cttgtaagac tcttaaaagt
     4441 ttaacctgta aatcatgtgg catatctcct atctcatcta aaaatatagt gccagtgtga
     4501 gctaattcga ataatccttt ttttccacct ttttttgctc cagtaaaaga accatcttca
     4561 tatccaaata gttcactttc aagtaattca cttggtaatg aagcacaatt tattgctaca
     4621 aaaggtttat tttttctatt tgaatgattg tgtattgact gagtaaaggc ttctttacct
     4681 gttcctgttt cacctgttat taatatagga ttatctgtaa gagccatttt ttttgccttt
     4741 ttaatacaat cttttataat agaactattt ccaataatat tattaaattt atatttagct
     4801 acataacctc ttttgacaga atcacttctt attttttgtt ctaatatttc tatattgttt
     4861 acatctttca atgtaattaa gcaaccctct atatttttat aaagataaaa tggtttttta
     4921 gaaagtatga attttttatc tatttcgtct atgtatataa tcatattatc gatatttaag
     4981 ttctttgtaa ttttatctaa gaaactattt ggcatatatt ccattaagga aggtgatttt
     5041 ataagttttt catctagtcc caacatatta agtaaagact tatttacaaa agtaaccttg
     5101 ttaaaggtgt tgaaaaatat tagaccatca cctatttcat caatggtcat gtaaagtata
     5161 gtttttaatt tagatatatt atctaatgat gtatttaaga aatcatgact tagtgtctct
     5221 ttggacaatt tgtatagttt ttctataaat ttctcatttt ttagttttaa tactactaat
     5281 aatgacatat atgtatctaa tgagattttt ctccaaccta aatcaacaat attttttatg
     5341 tattttggta taaatctaat ctgacctggt gttatagctg tatctaaatc atatatttca
     5401 tcacaatctg gatatgtcgg tacaaaatct atatgattta taccaatttt atatagataa
     5461 gtagccatct caaatgctga agttttattg gttgtcataa gtaacgattt tgtacctgga
     5521 ggtagtttca tcattttttc atatccaagt tttgaaagtg ctttcgttcc atgaataatt
     5581 tcaatatctt ccttaaaatg ttttattaaa aaattactag ttaaaggtga tagagaaagc
     5641 actacatctc cctctaaatt ttcttttatt ccatcaagta tattttgtgt ttctatgatt
     5701 atttcgtctc caaaaaagtt tgttaattct tctttgtagt ggtcactatt caatttatca
     5761 tgtgttataa ggattaactt ctttttatcc attttatttc tcctaacaaa atagtattta
     5821 tttttaaagt atttaaagta taaggcaagt tatattatac aacaaatagt agtataaatt
     5881 tatatatttg attaaataat aggttttttt aggattttat cctataaaaa cctattttaa
     5941 aattattttt gcaagat
//
LOCUS       DAMJWS010000138         5546 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMJWS010000138 DAMJWS010000000
VERSION     DAMJWS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.143"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(287..970)
                     /locus_tag="PC399_003538"
     CDS             complement(287..970)
                     /locus_tag="PC399_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF2594345.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAVIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(1146..1736)
                     /gene="zapA"
                     /locus_tag="PC399_003539"
     CDS             complement(1146..1736)
                     /gene="zapA"
                     /locus_tag="PC399_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF2594346.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1761..4154)
                     /locus_tag="PC399_003540"
     CDS             complement(1761..4154)
                     /locus_tag="PC399_003540"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF2594347.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4171..5190)
                     /gene="pheS"
                     /locus_tag="PC399_003541"
     CDS             complement(4171..5190)
                     /gene="pheS"
                     /locus_tag="PC399_003541"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF2594348.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5240..5474)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1685 a         1093 c          604 g         2164 t
ORIGIN      
        1 tacttgaaat acttctatta atctatatat tgcctactat acttaagaca ttttaattaa
       61 tttatacact gtttactata cttgaaatac ttctattaat ctatatattg cctactatac
      121 ttaagacatt ttaattaatt tatacactgc ttactatact taaaatactt ctattaactt
      181 atacattgct tactatattt taaattcttt aattaatcta tgcaatattt gctatatata
      241 aagtatatca aataacagct tcattttttt gtaaatttac agaaagttaa ttttcattaa
      301 atgaatcatc tttattgata aacataattc caaagaccac tgccaaaata aaaaaacata
      361 ttagtattga tttttccacg aaaatttttg taaaaaaagt tcctaaaata gctccagata
      421 taaagaatat aataacagca taatactgta aactcttttt tatcataaaa atattcttag
      481 tatgtatata atgaaataaa gtttctgtag cacttcttaa atttccagta cacattgtcg
      541 ttgcaaaact attcccattt acttttctaa aactctctac ttgcattgaa caaataaaag
      601 aaatagcaac atttacaaac atattaaatc ttcctctagg aataaatgaa acaataagta
      661 ttgtaatcat ttcaattata atgataacct gacgccaatg aatctttgtc ttattatttg
      721 taaaatactt tcttatcttc tcggctataa atacacctaa cataaatgcc aaaataggaa
      781 tcaaataata aaatgattcc tttacattac cttctgctat attaagccca aacaaaacta
      841 tatttccagt ttgtgcatta gcaaagactt tacctcttac cacataagta taaatatcca
      901 aaaatcctcc tgttattgcc aacaaagaac ataaaaagaa tgattcagac atctgactct
      961 tgcttgtcat atataaacct ctcgctttct aatgatagat acaaaatcaa aatcatttta
     1021 gattctattt ttcattgata taaaaaaatt atttatattt aaatattgtc tatatttata
     1081 catcatttat atttatacat taaaagaagc tgtctcaaaa aacaagagac agcaaatctt
     1141 ttagtttatt ccacattttt tgcatcatta tttaaccctg tacattttaa ctgtaaatca
     1201 taagctttgt tttgaaactc agaagctaag ttttcagcta tagtagctct ttcatttgct
     1261 tcttcaagtt gagtacttaa ctcctcaatc ttgtttttat aagcatctaa ctctgctcta
     1321 gaaccttctg tcgtattact aagttcgaat atttcttgct ttttattttc tataatctca
     1381 attaactctt tcatcttagt ttcagcttca cgattttctg cttctttttc tgctatcgta
     1441 agctttaaag acttgatttc taattgaagc tcttcattag atgctccaac cttcttactc
     1501 aattcttcat tagctttaat caacttctca ttctcatctg aacattcaaa taaaaggtct
     1561 gatatattta atgcagttaa tatcgcagca actgataagc tcaatttcgg gttttgacgt
     1621 gttattttat ccatttcttt gtctacaaaa tcagcaatac taatcataaa cttctctgat
     1681 ttgtctccaa ccattggata ttcagcgcca tggattttaa ccattacttt gttcatccta
     1741 acctcccccg acattagttg ttaatttgat cttaagttag catttaattt ttcgcttaat
     1801 tcacttaata tcttatcatg caccttagct acatcttcat ctgttagagt tttatcttta
     1861 cttctataag ttattgaata tgcaattgac ttgtatccag cttctatttg agaacctttg
     1921 taaacatcaa ataacttgta gctttctact aatccttgac cattttcctt tattatatct
     1981 tctatttgtt ttacaaatac atcatcttta acaataagag ctatatctct tgatgtagat
     2041 ggatattttg gtaatggcac ataattcttt gttctatcag aattatcaaa tacaaagtca
     2101 atatctattt cagcgacata aactctttga cctagattgt agttttctat aacatctgga
     2161 tgcaattcac ctaatgtacc tacatattta ttattataca ctattttagc acatctacca
     2221 gggtggaagg tagtattatt atcttgaggt tcaatttcat aacctttaaa tccgacattt
     2281 acaagtatag tttctatagt tccttttagt gcaaagaagt caacatcttt accatacata
     2341 cctacacaca ttctattttc ttccttaggt aattctgaat cttgtgggat gaatatatgt
     2401 ccacactcaa atgcagatac ttcttctatt ttatgagata cattagttga taaaacatct
     2461 aacatatttg gtattaaagt agttctcatt acagaagttt cttctcctaa tgggttaagt
     2521 aatttaacaa aatttctctt tgcattacct tctggtactc ttatcttatc tactccctta
     2581 gggcttacaa atgaatatgt taatatttca tttaatccta cagcagttga attatctttt
     2641 aaagcatcca tgaatttttg ttttgaagtt ttaattcctg ctgtcgtatt tccttctaat
     2701 tgtacagaag gtatattctc aaatccataa attctagcta tttcttccca tacatcagct
     2761 tcttgttcca tatctgttct aaagcttggt acatctattt ctaatttatc attagctacc
     2821 aaattacatt taaactctaa tgattctaaa attcctacaa actgctccat tggtacatct
     2881 acacctaata gatggttaat tctttgagga tttactacca attttttagg ttctggttta
     2941 tttggatata catctacaac accttttaaa acttttcctg ctcctaacat ttcaacaagc
     3001 tgtgcagctc tatttgctgc tatctctgca agattagggt ctaagtcttt ttcatttctt
     3061 gaagatgctt ctgacctaat accaactttt ttagctgtca ttcttatgtt ttctggtttg
     3121 aaattggcac tttcaaaaag tacagtcttc gtattagaag ttatttctga gttagcacca
     3181 cccattacac cagctaaacc aagtgatttt tctccatttg ttataactag catatctttg
     3241 tctaatgttc tctcaacatc atctaatgtt acaagtttct ctccatcttt agcatttctt
     3301 actactattc ttccagtctc tacttgattt atatcaaaag catgaagtgg ttgaccaagc
     3361 tctaacatta cgaagtttgt tatatcgact atgttactta taggtcttac tcctgcttct
     3421 gtaagtcttc tttgcatcca atatggagaa ggttctattt ttacatctgt aaccattcta
     3481 gcaacatatc ttctacataa atctggatta tctatctcaa ctttgaaatc tatctcttcg
     3541 tcactttctt ttacttcgat ttcaggatat tttacttttg ttcctatagt tgcagctgct
     3601 tctctagcta tacccatcat gcatttacaa tcaggtctgt ttgaagttaa ttcaaaatct
     3661 attaaagcat cttttaatcc taaaacatct tttatatctt ttcctaattc ataagaatct
     3721 tcgtggtcta aaatatatat accacctctt ttatactcct caatgtaatg ttcatctata
     3781 cctagttcag ctgctgaaca catcatacca tctgataatt cacctctgaa atcagtctgt
     3841 ttgatttcaa ctcctccagg taacttagaa ccatttacag ctactggaat ataatctcct
     3901 tctgatatat ttgtagctcc tgtaactatt tgaacaactt tatctccaat atctactttg
     3961 gttacaacca acttatcagc atttggatgt tgttttattt ctaaaatctt tccaaccaat
     4021 atattttcta tttcttcacc ataataatct attgtttcaa ctttagttcc tgtcattgtc
     4081 attttatcag cgaactcttt tacatccatg tctatatcaa catagtctct aagccatttt
     4141 aaagatacta acatctacat accctcctaa ttaaaattga tttaagaatc tcatatcatt
     4201 ttcgaataat aatctaatat catctatttc gtatttaagc attgcaagtc tttcaacccc
     4261 aactccaaat gcaaatccac tgtaaacttc tgggtctatt ccacaatttc taagcacatt
     4321 tggatgaacc atacctgaac ctaatatttc tatccaacct tcatatttac acattgggca
     4381 acctttacca ccacatttga aacaagtaac atcaacctct gcacttggtt ctgtaaatgg
     4441 gaagttgtga ggtctaaact tagttttgat atcagaacca aacaattttt caacgaagat
     4501 atccatagtt cctttaaatt ctgccatagt aacatctttt ccaacaacaa gcccttctat
     4561 ctgatggaac attggtgagt gtgtagcatc tggcgagtct gacctaaaac atctacctgg
     4621 tgcaattact tttattggta actcttgact tctcatagtt cttacttgaa ctggagatgt
     4681 ttgagttcta agtaataccc catcattgat atagaatgta tcgctcatat ctcttgatgg
     4741 atggtcttta ggagcgttta atgcgtcaaa gttgttttca acagtctcaa cttctggccc
     4801 ttctgctata gagaatccca ttccgataaa tatatctgtt acttcatcta taatttgagt
     4861 tattggatgc ttcttcccaa cctttaaaac ttttcctggt tgagtaacat ctatcacttc
     4921 ctcttttaat tttctttctt tttcaatagc atttatttct tcttttttag aatttatgct
     4981 aagttcaatg ttttctctta cctcattggc aaccttacca actactggtc tttcttcagc
     5041 agataattta cccatttctt taagtatggc agttatctca cctttttttc ctaagtactt
     5101 aactcttaaa ctttctacac tttctatgct ttgtgcttct tttatttcag ccaaagctgc
     5161 ttcacgtaaa gcaagtaatt tttcttgcac acatatcacc ctttcgtttt tttgtataaa
     5221 aaaagcccct caatccctgt ctagggacga aaggctatag tttcgcggta ccaccctagt
     5281 agtttaacaa tttaaaaatc atcaaaccac ctctaacaaa cttaacgcgt ttttgacgtc
     5341 aaagcctact gtaaaatttc agctctgtta ctccagaggg aacttctcat gacctcttag
     5401 aaaacactcc cagcctaggt gtttatctct ttactaagtt taatcaaaat actttcctct
     5461 ttcattgcat ttacaatatt atattgttta ttttagtatt atatcatttc cattcaaatt
     5521 atgtgactat ttttaatata tatcca
//
LOCUS       DAMJWS010000139         5525 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMJWS010000139 DAMJWS010000000
VERSION     DAMJWS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.144"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            50..166
                     /gene="rrf"
                     /locus_tag="PC399_003542"
     rRNA            50..166
                     /gene="rrf"
                     /locus_tag="PC399_003542"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(380..1564)
                     /locus_tag="PC399_003543"
     CDS             complement(380..1564)
                     /locus_tag="PC399_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2594349.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1937..4288
                     /locus_tag="PC399_003544"
     CDS             1937..4288
                     /locus_tag="PC399_003544"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2594350.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4310..4849
                     /gene="nrdG"
                     /locus_tag="PC399_003545"
     CDS             4310..4849
                     /gene="nrdG"
                     /locus_tag="PC399_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2594351.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAKEAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKQVFLVEQYMKDDLSIAE"
BASE COUNT         2151 a          625 c          888 g         1861 t
ORIGIN      
        1 ttctataaac ttattaaaaa cttaatagaa atattaagca taaagattat gtggttacaa
       61 tagcagagag gatacacctg ttcccattcc gaacacagaa gttaagctct ctagcgctga
      121 tggtacttgg tgggaaactg cctgggagag taggacgtag ccacgtaatt tttttgttat
      181 aataaattta gaatttaact tatatagttt aatttgataa tttgatgaaa aaaataaaag
      241 acttattgat ttttaaatct ataggtcttt tattttttat ctaacattta aattaagttg
      301 ttgaatatat cttatattac ttaccttata tattaattat cattaataca ttatatctaa
      361 ttactattca tctaaattat tatgccatta ttttatatac aaattctttt aatttatcta
      421 atccagataa gaaagtattt tcactgcaag cataggatat acgaatatat ttatccatac
      481 caaatgctat gccaggaaca actgcaacat tatattcttc taaaaattga ttacaaaatt
      541 ctatagaaaa actatcttta tattcaaatt tctcagatac tttagaaaga tctataaata
      601 tatagaatgc gccatttgga ttcacatatc ctacattatc aatagagtct agttttgatt
      661 taattaaatc tcttcttgat ttatatgttt taaccatatt gtctatgtca attgagcaat
      721 ctttcaaggc tccataggct atgtattgtg ctgttagact tgggtgagat ataagatgtc
      781 cttgtataga actcattgct ttagcaatcg ttttattaga tgcagtatat cctaatctta
      841 atccagtcat tgcagctgac ttagaaaaac catttatagt tattgttata tctttagctt
      901 cttcgcttaa tgatgcaatt gatgtaaatt caccagtgta gcatattttt tcatatattt
      961 catctgctaa aatatatatt ttattttgta tgcatacatc tactatttct attaattcat
     1021 cttttgtata aacactacca gtaggatttg aaggattgtt tattacaaga atttttgttt
     1081 tatctgttat agatttttca agttcttctt ttgttaactt aaatccattt tcttttttag
     1141 tatcaataaa aactggaaca gcattaacta acttaatcat ttcaggataa ctaacccaat
     1201 aaggctttgg taataagact tcatctcctt cgtctgttaa agctagtaaa gtatttgtta
     1261 ttgagttttt tgctccactt gatacaacta tttcgtctat tgaatagtta cagttatttt
     1321 cttcaataag ttttttacag atttcctctc taagtatttt taatcctgga actaaatcat
     1381 atttagtgta atctttattt aaagaatcaa taccataaga ttttgcgtta ttaggtacat
     1441 taaaatcggg ttctcctata ctaagattta ttactttaac tccattactt tccatttctt
     1501 taactttaga actaatacct attgtgtagg atggtgttat aaagtttagt ctttttgata
     1561 acataaaatg cctcctttta aattattatt tatattatac gacaaattta aagaatttac
     1621 ataaggaaat ttgtagatat atacattaat atatataata gcctacactt tttgcctaaa
     1681 attagtgtaa aaaaaagaga aaaaaatttt aactttcaaa gttgttagaa acgctactgt
     1741 tcaaaatatc cacatagtat ttgcaatgat ttgcgcaagt ttgactatta tgtacaaatt
     1801 tggtaatctt aatatagcaa ttattaagat aatcccttta acttagttga aggggatttt
     1861 tttagactta acatactaca tatagtggta tggggattaa taaaatacaa tatatagtaa
     1921 caggaggttt ttttaattgg ttgaattcgt aaaaaaaaga gatggaagag taattccatt
     1981 taatgaagat agaataacaa gagctatatt tttagcagca acgaatgttg cagaaagaga
     2041 aggaatagta cctgattata aattatctga gcaattaact caagaagtaa taaagttttt
     2101 aaatcataaa tactcagaat cagttccaag tgttgaagat atacaagatt ctgtagttaa
     2161 ggttcttata gaaactggcc atgcaaaaac tagtgaagaa tatataatat acagaactga
     2221 aagaagtaga ataagaaatt caaaaacaag gttgatgaag gcaatagagg aaattacctt
     2281 tgaagatgca gaagatgctg atataaaaag agaaaatgca aatattaatg gtaatacagc
     2341 aatgggaact atgttacaat atggaagcac tgtatctaaa gaattttgta aaacacatat
     2401 attaaaacca gagcattctt ttgctcatga taatggagat atacacatac atgatatgga
     2461 ttttttaaat atgggaacat taacttgttg tcaaatagat gttaaaaaac tattcaatgg
     2521 tggattttca acaggacatg gctttttaag agagccacaa gatataataa gttatggtgc
     2581 attagctgca attgctatac aaagcaatca aaacgatcaa catggaggtc aaagtatacc
     2641 atttttcgat tatggattgg ctgaaggtgt atataagaca tttaagaaat tctacatagg
     2701 aaatttagct aaagcattaa agttatttaa aggaattgaa aataatgatg tgataaagaa
     2761 catagtatat aatacagaga aagaaacaaa tcaaaaagtt ggtcttaaga gagatgaatt
     2821 gtatctaaac ttagaaaaag agaaattaat acagactttt gatatagatg atgaacttgt
     2881 aaataagatg caaaactttg catttgaaga atcgtataga gaaactgata aaaaaacata
     2941 tcaatctatg gaagcattta tacacaatct aaacacaatg cactctagag caggtgcaca
     3001 ggtaccattt tcaagtgtaa acttcggaac agatacttct gaagaaggaa gaatggtaac
     3061 taagaatctt ctattatctc aagaaagagg tttaggtaat ggagagacac caatttttcc
     3121 aattttaata tttaaggtca aagaaggcat aaatttaaat cctgaagacc ctaattatga
     3181 tttatttaaa ttatcatgca gagtatcagc taaaagacta ttccctaatt ttagtttttt
     3241 agatgcgcct tttaatgcaa aatattataa aaaaggtgaa ccagatacag aagctacata
     3301 tatgggttgt agaacaagag tacttagtaa tgtgtgtggt tcagaaacag ttagtggtag
     3361 aggaaacata tcttttacta cagtaaattt accaagattg ggtataaaac atggtattat
     3421 aaataatgaa aaggctaatt tagacggttt ctttgaagaa ttagatgaga agataaactt
     3481 aattatagaa caattattgg agcgccttga ggtacaagga aataaaaaga tgaagaattt
     3541 tccattctta atggggcaag gtgtatggaa aggttctgat gatttaggac cagaagatac
     3601 actaaaagaa gtaataaaac aaggaacatt gacaattgga tttataggtt tagcagagtg
     3661 ccttattgct cttataggta aacatcatgg agaaagtaaa gaagctcaag aattaggatt
     3721 aaaaatagtg tcacatatga gacataaaat ggatgaagca acggacaaat ataaattaaa
     3781 tttctcctta atgggtactc cagcagaagg tctttcaggt aggtttacta aaatagataa
     3841 aaaagtttat ggagaaatta aaggtatcac agataaagaa tattatacta attcattcca
     3901 tgtaccagtt tattacaata taagtgcata tgataaaatt gagatagaag ctccatacca
     3961 tgaattgact aatgcaggtc atataacata tgtagagtta gatggagatc catctgataa
     4021 tttagaagct tttgaaactg ttataaaagc tatgaaagat ttgggtatag gatatggaag
     4081 tataaatcat cctgtagata gagacccaat atgcggtttt tcaggtgtaa taacaagtaa
     4141 tatatgccct gtatgtggaa gaaatgaaga tgaaagtgat atcaaattcg aaagaataag
     4201 aagaataaca ggatatttag ttggtacagt tgatagattt aataatgcta aaaaagcaga
     4261 agtaagagat agagtaaaac atagatagtt ttatttgggg gaataagtta tgaaaataag
     4321 aatgtcatct actataagtt atgacagcat agttgatgga ccaggattaa gaatggtaat
     4381 atggacccaa ggatgtattc ataattgtaa agagtgccat aatcctcaga cacatgattt
     4441 atgtggagga ttttatatgg atacagaaga aattataaat aaagtcaaat cattgaaatt
     4501 acaaaaaggc attacgctat caggaggaga accattttta caaccagaac cattagaaga
     4561 aatagcaaaa gaagctaaaa taaatgggtt agatgtatgg tcatatactg gatttacgtt
     4621 tgaacaactg ttagataaaa aaaatagagc gtattttaaa aatttaaatt tactaaagca
     4681 aattgatatt ttagtggatg gaaaatttat agctgaaaaa aaagatataa gcttaaagtt
     4741 tagagggtca tcaaatcaaa ggataattga tgttcaaaaa agtttgaaat ataaacaagt
     4801 gtttttggtt gagcaatata tgaaggatga tttatctata gcagaataaa atataaataa
     4861 acaggattaa atatatgcat atatttaatc ctgtttattt tcatataata tttcaagctt
     4921 gtataatgta acaattaaac caaaagactc attgtatatt tgaaattaag ttttttatag
     4981 tttataggtg ttttatgtgg attacgaatg atatagtatt gatacattat ttaatagatt
     5041 cttatgccaa gaaacaatta atttatagaa attcttaatt gttacaatat aaatatctag
     5101 atatgttata agatactgat aaataattta taaagtttat taatctaata ttgtgtgtga
     5161 atattaagtg gtcaagtagt ctaaaaaatg aaaaagattt aaaaaagtgt tttaactaaa
     5221 cacataagtg ttaaattcat gtataagtat aggttagata cataagaaat aattatgtaa
     5281 tatctatctt tgtaaaaaaa tattaaattt tgtaatttgt ggataaatat gtttataatg
     5341 tggataatat ttaatgtaat aagttaaagc aatatgaatc aactgtttat aatgtggata
     5401 atattataaa tgtatgaata attgcagtat ttaaaaacaa aaaaagttaa aaaagttgtt
     5461 gactctaaaa gaaaaagttg gtatagtaat acttgtcctc aagaaaaggg caaacaaata
     5521 aagca
//
LOCUS       DAMJWS010000140         5517 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMJWS010000140 DAMJWS010000000
VERSION     DAMJWS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.145"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            186..692
                     /locus_tag="PC399_003546"
     CDS             186..692
                     /locus_tag="PC399_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF2594352.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            1105..1773
                     /locus_tag="PC399_003547"
     CDS             1105..1773
                     /locus_tag="PC399_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594353.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSGKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            1932..2438
                     /locus_tag="PC399_003548"
     CDS             1932..2438
                     /locus_tag="PC399_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2594354.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            2566..3582
                     /locus_tag="PC399_003549"
     CDS             2566..3582
                     /locus_tag="PC399_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2594355.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            3936..4640
                     /locus_tag="PC399_003550"
     CDS             3936..4640
                     /locus_tag="PC399_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF2594356.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            4935..5414
                     /locus_tag="PC399_003551"
     CDS             4935..5414
                     /locus_tag="PC399_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2594357.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         2298 a          472 c          918 g         1829 t
ORIGIN      
        1 atattttatc tatattttac acttataaaa tatgaaaact acttattgcg aaagtaattg
       61 atttgttgta ttataattta catgaattat gtaggacaat caaaagtcag tattataaga
      121 gtacatatat tcaaattttt aacaattaat ttatataatt aaaaaatgaa gggtaggatg
      181 aagtcatggg ttttagagaa gtaaaaattg aggaattaca gtttaatcca tttactaaga
      241 ttggcaaaga atggttactt ataacagctg gtgattcaga aaagtttaac acaatgactg
      301 ctagctgggg tggtgttgga gtctattggg gtaaaaatgt tgttacaact tacataagac
      361 cacagagata tacaaaagaa tttgtagata gtaatgatac atttacaatt gccttctttg
      421 atgaaacgta tagagaagct ttaaatattt gtggcactat ttctggtaga gatataaata
      481 aaatagaaaa agcaggactt acaccatatt ttgtggatga tacggtagct tttgaagaag
      541 ctaatatgat tatagtatgt aaaaagctat atcatggtaa tatgccacca gaaaactttg
      601 atgctaaaga aaatgataaa aaatggtatc cagagaaaga ctatcatact atgtacattt
      661 cagaaattat aaaagttctt gtaaaagaat aattataata aaaaatatat gaaaagaact
      721 tattataagt aaatgactgt gctttcttat ctgatatcta agtagatgct actaactaaa
      781 taaatattaa ttttaaataa atactattat aaaaaagatg aactatattt actatagttc
      841 atctttttat ttatataagc acttatataa gtagagattt atttgaataa aagtatttat
      901 ttgaaatatt tgagtatact ttataagtat ttttatgcta actagaaaca cttaatttat
      961 ggcatttgta cttgatttat tttaatgtaa ataatttcaa agttaatttt attcaatata
     1021 gtactataaa agtataaata acatttaata aaaatatata ttatattagg tattttaaat
     1081 aaatttttta aagggtgatt ttttatggaa aatataacta gttattttac aacaatacta
     1141 tgtgtattta tatgcttatc atctgtattt atatttactc aactagctag agtatttatt
     1201 aataagaaga agattaatca aaaaattaaa tcaagaaacg gatttaggta tgatagagat
     1261 tttattgaag ccagaagaga agaaatacat ataaaggata acaataataa taaaaataag
     1321 agtaacaata agaaattaaa ggaagaaaaa gtatttaaat acgataatgg tgatttatat
     1381 aaaggtgagt ttgttgatgg aaagaaaaat ggatttggaa tatatatttt ttcaggtaag
     1441 gaaaagtatg aaggattatg gaaagatgac aagatgcatg gaataggaaa atatacttat
     1501 agagatggtt caatatatac aggtgaattt aaatacggat taaaaaatgg tttgggaaaa
     1561 ttaacatatc ccaataatga catttataag ggatattttt tagataataa gaaaaatggt
     1621 aagggtgtat tatataaaaa tgatggaaat aaacaagcag gaatatggga agatgatgaa
     1681 caatgtaaaa gtttagattt taaagattta aataataata aaataaataa tgtttatcaa
     1741 aatagattaa ataacaataa attaaaagtt taattattct aataaaaaaa tctttattta
     1801 tgatggaata taggatatat ctatgttgta atttgtaaaa tcttcctata tcatataaaa
     1861 ttaagtattg attagtctat tacataaata tagaatataa atatatccat ttaataataa
     1921 ggagaagaga tatgaaagag tatgtagttg tggatttaga aactacagga ttagatccat
     1981 ataaaggttg tgaaataata gaaataggaa taacagaaat aaaaaatgaa caaattgtaa
     2041 aaaactattc aaggttgata aaacctaagg ggataatttc ttcatttata acagaattga
     2101 cacatataag taatgaaatg gtagaaaatg aagaaccact agaattagtt ttacctagat
     2161 ttagaaaata cataggtgat agaactataa ttgcacacaa tgcgaaattt gatttaaaat
     2221 ttttaaatta ctatcttaga atgttagatt tagaaccaat aaacaattat atatgtacag
     2281 tagagttatt aaaaaaatgt aaaagttata aaggaaaaaa caagaaactt gaaacagctt
     2341 gtgcttatta caacatagag aatataaatg ctcatagagc tgacagtgat acattagcaa
     2401 ctgcaaaact atttttgaaa ataaaagatg aatattaatt ataaatatag agaaaattag
     2461 tgaaaatatc ctttataaaa ttgaccctta agcttacaat tttgttcttt gatttatttt
     2521 gtagaacaat gtataatgta ccttatatga agaagggaga ctaaaatggg ttattacaat
     2581 gataatgata ataataaaat aagacgtaaa aaaattagta acaacaaaga tagtaataaa
     2641 aaatctaaac attcttatac ttcggcaaat aaaaaaaata aaagtcctaa tacaagttcc
     2701 agtaaaagaa tgagtgaaaa agtagattca cagaatctta taaaaagaga tagagatagt
     2761 tttacaagag ataatgtaag acctttaaat acagattaca atcaaagaga aagaagaaga
     2821 gtcgagtata aacaaagaga gaaaaaatta aaaagtaaaa aaagaaaaaa acaaaaaagg
     2881 attagaatag ttagggcatt attatctatt atgttagttg cactaattat attttcagtg
     2941 gcaggtgcaa tatttacagt ttcagctata aaagggtcac cagaagttac taaaaagtta
     3001 attaggtcta aatatataag tagtgaggtt gtaagtataa aacaaatgcc aaatgattta
     3061 aaacatgcaa tagtttccat agaagatgag agattttata agcataaagg agtagatgtt
     3121 atttctctag ttaggtcagt attaaacaat gtgtttacag atacaacaca aggtgcaagt
     3181 actatagata tgcaagtttc aaaaaacctg cttactagta atgataaaac tatgaagcgg
     3241 aaaataaggg atatgtataa tgctaagcag atgaataaga ttatgactaa ggatgaaatt
     3301 cttgaagcat atttaaataa tatgtatcta ggtaaaaatg catatggagc tgcaaaggga
     3361 gctgaagtct actttggtaa aaaagttagt gaattaaatt tagctgagtg tgctatgtta
     3421 gctggtatga caaataatcc tgcaaggtat attgaccatg gagaggctaa gaagagacaa
     3481 gtgattgtac tgtataagat gcatcagctt ggttatataa ctgataagga gtatagaata
     3541 gcaaaagctg atgacactcc atttaaatca gaaattgatt aaaattgtaa gaatatagta
     3601 ttttcaatag tttaaacaaa caaaaatgta ataaaaaagg tgatttgcca cccatttgcc
     3661 accataatta taatctaatg gcaaattgtt ttaatgtaca aactagataa tagaatattg
     3721 tcttaaatgc tatattatgc taaaatttaa ttgtattttg taaattaaaa aacttataat
     3781 ttagttttct agggttccgc attttataat ggtcaggtcc aagagaaaac gcgcaattgt
     3841 tgtgtacacg gaaggataaa agcctgggag atatttaaga atatttctat aggctttttt
     3901 atttttacat attttaaatt tgaggaggtt ttctaatgaa aaaatttgtt attgaggatg
     3961 cattttggaa tctatttcct agtgcaaaaa ttggagttgt tatttgtaat gatatagata
     4021 actctataaa agatgaagac tataagaaaa taattttgaa gggagaagaa gaagcattaa
     4081 aatacttgga ggactcagaa tttagcaaca accaagtgat taaagtatgg agagaagcat
     4141 ttaaaaaatt taaaacgaaa aaaggagcaa ggtcttctat tgaagcatta ttaaaaagag
     4201 ttcataatgg aaataatata ggaactatta accctttagt ggatatctac aattttatct
     4261 cattaaaata tgcattacct tgtggagggg aggatataga taaatttata ggagatatta
     4321 ggctaactag agcaattggt aatgaggagt ttatcccact agggacagat gaaaattcat
     4381 ttccatatga aggagaaata atttataaag atgatggtgg agcaatttgt agatgttgga
     4441 attggcgtga agcagtgaga acaatgctta ctgaagatac taacaatgcc tttttatgca
     4501 ttgaattagt agatgaaagt agatttaaag aatttgaaaa tgctttaaat gaattagcta
     4561 aaatagttca agaaaaattg ggtgggaatt gtaaaatttc aattttagat attgataata
     4621 aagaggtatc agtggattaa tatggaggtg ctgttgtaat ggactattct agttataaag
     4681 atacttatat ttagggtaag aaggtctatt ttagatataa cgattatcaa aggatataat
     4741 atagaaaata aaaatgtaga atataaaaag cctttaaaat aagaaaaaaa tgtattttaa
     4801 aggcttttat tatactcttt tttatttgca taattaacaa taaaaatata ttgttaatta
     4861 taatattttt atttaaaaac gatataatat aatgtattat actatatata aaatatttta
     4921 aaggagttgg catgatgtct aataaactta taaaaaattt actaagtggt atattacaaa
     4981 ttttattttt tcttttagga ttagtaattg ttgtaggagg atttaaaagt tttatgtacc
     5041 tctgttttag tggagaagca actctacaag gtactattta tggaatatta atgtttattt
     5101 taggtgtatc atattttatc attataaaat cattgataga agtattaggt agttctgaat
     5161 atagcctatt tgtaaaagag aatgtaaaaa gatttaggat tattggatat cttttgcttt
     5221 taaactctct aattgagttt atttcaactt ttggtactac tggaaaagga atgagatttc
     5281 tagatttagg atttggattt tattttactg ttccagtatt tgtatatttt ataacttctc
     5341 tgatgagttt tgtaattgca gatggatttg taaaggcaat aaagataaaa gaggataatg
     5401 atttaactat ttaggtgtga cactttaata tataggagat aaataacccg aaaagagtga
     5461 ctaactagta atttaaaata aaatatatta gggtataata cataaactat aatgcta
//
LOCUS       DAMJWS010000141         5410 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMJWS010000141 DAMJWS010000000
VERSION     DAMJWS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.146"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            164..499
                     /locus_tag="PC399_003552"
     CDS             164..499
                     /locus_tag="PC399_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594358.1"
                     /translation="MIKLWKKEDLNLLLEYPKEVVENVDNVINILDENYGCNRKLTDD
                     GGYVCIIEDIKEVENLKSNILKGLVEEFSDVIYEDEVNTYNSTLYLLSSDYSVTVISK
                     NEETEYLLK"
     gene            563..1021
                     /locus_tag="PC399_003553"
                     /pseudo
     CDS             563..1021
                     /locus_tag="PC399_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            1161..1454
                     /locus_tag="PC399_003554"
     CDS             1161..1454
                     /locus_tag="PC399_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594359.1"
                     /translation="MFKKENRYMTKEIAENLPIEIAILLWNLIDDLTTEKDYLQLFEI
                     NPIGLGVVEIVHKQEVPEYEARIYIQNDKIKDKLKIYVIDNVEYRTMMFSNEY"
     gene            1481..2077
                     /locus_tag="PC399_003555"
     CDS             1481..2077
                     /locus_tag="PC399_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594360.1"
                     /translation="MINNKSEIINKLFFNELKELIDKYNNIDDKTVDVIEKIFTCIED
                     EEIKNYLIRDNCKLQELVNTYNKLEDLNIDEAIFFAWYNLTINTISIDKASEYYDDLT
                     TSNYIEVENHIIYTNERDLRKYAEDELETMLSMEHHIDRLLHKDMLIDMWLNNTSKEE
                     VIEEILMSDDIEDILNLSPEYAFTLANGIEYRYSEKEE"
     gene            2230..2499
                     /locus_tag="PC399_003556"
     CDS             2230..2499
                     /locus_tag="PC399_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HDF2594361.1"
                     /translation="MTNNNNNCDYKGIYADMVEVLGEEIVIKLHKYYRGQQITFPMKL
                     YSIEYVERYIVKNYRTKTIKEMCRELGYTEGWIKQLINKNRLNSK"
     gene            2525..2767
                     /locus_tag="PC399_003557"
     CDS             2525..2767
                     /locus_tag="PC399_003557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594362.1"
                     /translation="MILTVLAILMGTGIFFLINSMLDITYFGCGPIGFLWFGCFIVSY
                     AILTSLGGIVLGLLKWIVIGLIILFVIGLIANKIND"
     gene            2973..4106
                     /locus_tag="PC399_003558"
     CDS             2973..4106
                     /locus_tag="PC399_003558"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012938.1,HMM:NF016983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2594363.1"
                     /translation="MQDFDFISETIKKFQSDLENMDKVNILVVGKTGVGKSTLINNIF
                     REELAETGIGRPVTQHLKKISKEGLPINLYDTKGLELEEDVQETIKKEILDEIENCNR
                     NSLERNDKSELMHVCWYCINTSVARCEETDIKWINELSEKIPVIVVLTQGFPRKKARE
                     LKSSIDDLNLNCKNVIPVLAEQLVEEEDEIVIKSYGLDKLVEVTYQVIPKEVKKAFVN
                     VQKVDIKKKVEESRKWVLGYVGGSGAIGFSPIPFSDAPLLATAQVAMISHITVIFGLQ
                     VDKALLTAIVSSIGGVTSATLLGKTLVSNILKFIPGAGTIAGGAISGSVAALTTTALG
                     MAYISVLEYILNENMKGNEVNNTEIAEKMKDAYKDQLGMLSKK"
     gene            4870..5115
                     /locus_tag="PC399_003559"
     CDS             4870..5115
                     /locus_tag="PC399_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594364.1"
                     /translation="MRSVTVEDGDERFAIEKIYIKSLQRYEVRICLYRDCRDRINKLI
                     PRPVDLTMDKFVALILVGLKAGVLDDDFKQELIKGLA"
BASE COUNT         2330 a          452 c          963 g         1665 t
ORIGIN      
        1 agataaactg tttttaatta aattataaat atatattata ttaatgaatc tatattaaat
       61 agatagttga gtaagtagta ataccaaaaa tattaaaaag agatgttggt agaaatacga
      121 acatctcttt tttagtgcaa aaaattaaaa ggaggaagtt aatatgatta aattatggaa
      181 gaaagaagat ttaaatttat tattagaata tccaaaggaa gtagttgaaa atgttgataa
      241 cgtcattaat attcttgatg aaaactatgg atgtaacaga aaattaacag atgatggtgg
      301 atatgtgtgt ataattgagg atattaaaga agtagaaaac ttaaaatcaa atatattaaa
      361 ggggttagta gaagagttta gtgatgtaat atatgaagat gaagttaata catataattc
      421 aacactatac ttgttatcta gtgattatag tgttactgta atttctaaaa atgaagaaac
      481 tgagtactta cttaaataag catatacatt gataaggtgt atatgcttat tttaatttta
      541 ataaataaag gagaaaagca gaatgaataa aaagagaact cctgccaaga gagttgatat
      601 agttttactt aaattagtaa aagaacgtag tgttctatat gaaactagaa aaataagtaa
      661 tctttatgat gcttatagat tagcaaagaa ttttttataa atagtgctag agaaaagttt
      721 gtagtggtat gtttagatac aaagaattgg ccagtagcat agaggttgta agtataggca
      781 ctgtaaattt atctatggta tatccaagag aaatatttaa agtagctata ttaaccaatt
      841 catctgaaat gatatgcttt cacaatcacc caatgggtaa tactgacttt agcaaggaag
      901 atagttataa ctaatatgct acagaagcgt ggagaaatat tatgcattga attagtagat
      961 acatatagtt attgtagatg aggataaata ctttagcttt aaagaaaact gtaggatata
     1021 agagtcttag cttaaataaa ttaggctaag gctttttcta tgttccaaaa caattaaaaa
     1081 taaatcaaat ttaggttaag cccggaaagc ccttgaaaaa aataatacat ataaatttaa
     1141 aactaaatag gagttgtaac atgtttaaaa aagaaaatag gtatatgaca aaagagatag
     1201 ctgaaaatct accaattgaa atagctatac tactatggaa tttaatagat gacttaacta
     1261 cagaaaaaga ttatctacaa ctatttgaaa taaatcctat aggattagga gtagttgaga
     1321 tagttcataa gcaagaagta cctgaatatg aagctaggat atatatacag aatgataaaa
     1381 ttaaagataa attaaaaata tatgttatag ataatgttga atatagaaca atgatgtttt
     1441 caaatgaata ttagttaatg attaaaggag agagagtaat atgataaata ataaatctga
     1501 aataattaat aagttatttt tcaatgaatt aaaagagtta atagataaat acaacaatat
     1561 agatgataaa actgtagatg taatagaaaa gatatttaca tgtatagaag atgaagaaat
     1621 aaagaattac ctaataagag ataattgtaa gctacaagag ctagtaaata catacaataa
     1681 acttgaagat ttaaacatag atgaagctat attctttgct tggtacaact taactataaa
     1741 tacaatatct atagataaag caagtgagta ctacgatgat ttaactacaa gtaactatat
     1801 agaagtagaa aatcatataa tatatacaaa tgaaagagat ttaaggaagt atgctgaaga
     1861 tgaattagaa actatgttaa gcatggaaca tcatatagat agattactac ataaagacat
     1921 gttaatagat atgtggttaa acaatacttc aaaagaagaa gtaatagaag aaatattaat
     1981 gagtgatgat atagaagata tattgaattt atctccagaa tatgcattta cattagctaa
     2041 tggaatagaa tatagatact cagaaaaaga agaataaaat aagtatatgt actgaaaaat
     2101 acgggcttta agggcttata cattaaagta catatttgaa aataccatag attttacaag
     2161 agaaaatcag aatgaaactt tttcaaatac tagttattat taattagtgc tagaaaagga
     2221 ggtggagtga tgactaacaa caataataat tgtgattata agggcatata tgcagatatg
     2281 gttgaggttt tgggtgaaga aatagtcatt aaattacata agtattatcg tggacaacaa
     2341 ataacatttc caatgaaact atattctatt gaatatgtgg aacgatatat agtgaagaat
     2401 tatagaacaa aaacaataaa agaaatgtgt agagagttag gatatacaga gggatggata
     2461 aagcaattaa ttaataaaaa tagattaaat tcaaagtaat tagagaagga gattgacagt
     2521 agatatgata ttaacagtat tagcaatact tatggggaca ggtatatttt ttctgattaa
     2581 ttcaatgcta gatataacgt attttggatg tggcccaata ggatttttat ggtttggatg
     2641 ttttattgtg tcatatgcaa tattaacatc attaggtggt atagttttag gattgttaaa
     2701 gtggatagta ataggattga ttatactatt tgtaatagga ttaatagcga ataagataaa
     2761 tgattaaatt actgtagatt attttgataa aataattatt taatatattg aggcattaca
     2821 tgatagaaga ataaatataa atgacttaaa atctttaact taactaaaca aaaaatatat
     2881 tatttattac aaaaaatgac aaaaaaatta aatattatat tgtattatga caatataaaa
     2941 cattggagtt tataaattag gaggaatatt ttatgcaaga ttttgatttt ataagtgaga
     3001 caataaagaa atttcaatcc gatttagaaa acatggataa ggtaaatata cttgttgtag
     3061 gtaaaacagg agtaggtaaa agtacattaa taaataatat atttagagaa gaattagcag
     3121 aaactggaat tggtagacct gtaacacaac acttgaaaaa aataagcaaa gaaggattac
     3181 caataaattt atacgataca aagggattag agttagaaga agatgtgcaa gagactataa
     3241 aaaaagagat attagatgaa atagaaaatt gtaatagaaa ttcattagaa agaaatgata
     3301 aaagtgagtt aatgcatgta tgttggtatt gtataaatac atcagtagct agatgtgaag
     3361 aaactgatat aaaatggata aatgagttaa gtgaaaagat accagtaata gttgtattga
     3421 cacaaggatt cccaagaaaa aaagcaagag aattaaaatc tagtattgat gatttaaatt
     3481 taaactgtaa gaatgtaatt ccagttttgg cagagcaact agtagaagaa gaggatgaga
     3541 tagttataaa gtcatatggt ttagataaat tagtagaagt tacatatcaa gtaataccaa
     3601 aagaggtcaa gaaagctttt gttaatgtac aaaaggtaga tataaaaaaa aaggttgaag
     3661 agtcgagaaa atgggtttta ggatatgtag gaggatcagg agcaatagga ttttcaccaa
     3721 taccattttc agatgctcca ttattagcaa ccgcacaagt tgctatgata tctcatataa
     3781 cagtaatatt tgggttacaa gttgataaag ctttactaac ggcaattgta agttctatag
     3841 gaggggttac atcagctact ttactaggaa aaacattggt atcgaatata cttaagttta
     3901 taccaggagc tggaactata gcaggtggag cgataagtgg ttctgtagcg gcattaacaa
     3961 ctacagcatt aggaatggca tatataagtg tattagaata tatactaaat gagaatatga
     4021 aaggtaatga ggttaataat actgaaatag ctgaaaaaat gaaagatgca tacaaagatc
     4081 aattaggaat gttatctaag aaataattaa ttcaagataa agtataagat taattatagg
     4141 gaaaaagtaa aatttaaagg tatatttggt aaaacataaa caaaaatgat aatggcgtca
     4201 atattatttg ttgtcatatg tagtagctta gtatatatat tattgagtga taggtttaat
     4261 cataagttaa tttacttaga gaaagatata ttagatttaa gaaataaaaa tttgtaacta
     4321 gctaataaag gatcagtata gaaacagata aacaagagga taatgtagat attaaatcta
     4381 aaaacactaa taatgaagaa tataaaactt caaaactatt taggcactag atagagtatt
     4441 tgctataatg aataagctaa tagagaaatt aaaacgttta tagtaagata actaattcaa
     4501 atacatttag tggacttcaa ggaacatttg atacactatc aaactatggt agccaattga
     4561 tagaccaatc atctaaattg aatttatcaa atgttggatg gttagaaata tctgatataa
     4621 ttacaagtat aggaaaggac tattagattc tgaaaatatg tcatttgaag cattaaatat
     4681 aatgggaaaa ataggaaaat ttataaatta tattgaaatt aattatatta actaattaaa
     4741 tattgataca taatgccttg ctacagttaa atgtggcaag gcattatttt tgtaaagaaa
     4801 ggaagaatta acatggcgaa gcaaagtaaa gaggaactat ttgaaaaaga aactaaatat
     4861 tgcaagataa taagaagtgt aacagtagaa gatggagatg aaagatttgc aatagagaaa
     4921 atatatatta agtcattaca aagatacgag gttagaatat gcctgtatcg tgactgccga
     4981 gatagaatta ataaacttat tccaaggcct gtagatttaa ctatggataa gtttgtagca
     5041 ttaatactgg taggattaaa ggctggtgta ctagatgatg atttcaagca agaactaatt
     5101 aaaggtttag cttaggaggt agaatatgac aatagaagag tttactagtg gagtaatgtt
     5161 tgggatttca atttttagtg cagtattaaa gcaagtatta gaaaataaaa aatagtatac
     5221 aggaggtatg agtatgattg gatttataat aggaagtata ataggaattg gactatgtat
     5281 tagtatagag atactgatat cgaaaaatgc cataatatac taagttatat ttaaattatt
     5341 aatagattca tatataattt atttaagccc ttaaagccca gataagttat attaaagtaa
     5401 agtttgtatt
//
LOCUS       DAMJWS010000142         5361 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMJWS010000142 DAMJWS010000000
VERSION     DAMJWS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.147"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            154..990
                     /locus_tag="PC399_003560"
     CDS             154..990
                     /locus_tag="PC399_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2594365.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1343..2920
                     /locus_tag="PC399_003561"
     CDS             1343..2920
                     /locus_tag="PC399_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2594366.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGIANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2913..4097
                     /locus_tag="PC399_003562"
     CDS             2913..4097
                     /locus_tag="PC399_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2594367.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            4115..4714
                     /locus_tag="PC399_003563"
     CDS             4115..4714
                     /locus_tag="PC399_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2594368.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4738..5208
                     /locus_tag="PC399_003564"
     CDS             4738..5208
                     /locus_tag="PC399_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2594369.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2000 a          560 c         1029 g         1772 t
ORIGIN      
        1 ttaattgatg acaataaaaa atattttgtt attgtagtat tatagggtaa attgcaaaat
       61 atatacgaaa gtaaaatttt aaatattgac attaaattaa atatactata ctatgtaagt
      121 gtgatgagag aattttttta gaggggattt atagtgaata taaaaaagat atttaataac
      181 aatgtagtag taagctccct agaagatggc acagaaataa ttgtcactgg tgctggagta
      241 ggatttaaga aaaaagtagg cgatttgata gatgaaaatt tgatttctaa gaaatatttt
      301 gttcaagacg atcaaaggga caaatacaat caaatactta ataagacctc tattgaatac
      361 tttaaaattt cagaagaaat tatagaaaag gctaatgaag ttttaaatac tcaagtgaat
      421 gattcaataa tattagcctt aactagtcac atagaatttg ctgtccaaag agaaaaacaa
      481 ggtataaaat taccaaactt aatattgaat gagacaaaac aactttatag ggaagaattt
      541 gaatttggat tatgggcaat tgatgaaatt gaaaaaagga taggaataaa attgcctgaa
      601 gatgaagcag gatatattgc tattcacata atcaatggtt tggaaaattc tcaaaaagat
      661 gaaggtatta atatactaga gttttcaaaa caagttattc agataattga agaagtacat
      721 ggatttaaat tggatgtaaa ttcattaaat tatactagac ttataactca cttaaagttt
      781 tttgttcaaa ggattctccg aaaagaaact tacaatgata gtgatattga agatatgtat
      841 aagttagtga ataaaaatta caataaacct aaagtgtgta ctgaagaaat tgcagcattg
      901 gtattagaca gatttgaata taagattagt aaagaagaag aattatattt aatgattcac
      961 ataaataaaa taacaaacag tggttgttag gattgtaact gataaagcag gcaaaaccta
     1021 agtcattgag cttaactatt agattaagtt ttttgattta ggttttttta ttagatacaa
     1081 tcaaaataca gacagtgact tttaggattg taactggtta agcaggcaaa acctaaatca
     1141 ttgaatttaa ctattaggtt aagttttttg atttaggttt ttttattaga tacaatcaaa
     1201 tacagacagt gacttttagg attgtaactg gttgagcagg caaaacctaa atcattaaac
     1261 ttaattatta aattaagttt tttgatttag gtttttctat aaatttgatt atgtttttag
     1321 ataaaaatag gaggggaaaa ttatgaaaaa aaataagtta ggtttatgga acttcttcca
     1381 aatgttagga aaaacgttta tgttcccaat agcactactt agtgtttctg ggatgatgct
     1441 aggacttggg gcaggtttta cagaccctaa gatgattgaa atggttccat ttttagggaa
     1501 tgaatatgtt aacttagttt taaacttcat gttaacaata ggattatttg catttaacaa
     1561 cttgggagca ttatttgcaa tggcaattcc actaggatta ttaaaaaaag aaaaagagtt
     1621 tggtgctttt tcaggattag taggatttat agcaatgcat ataggtacaa atttttattt
     1681 aacaagagct gatttgttag taccagcaga acagatgtca actaatggtc aagctatgat
     1741 tatgggtata caaacatata atacaagtgt tttaggtggt attgtagcag gattaattgt
     1801 ttatgcaata tatgataaag ttagtaattt aaagatacca gaatcattag gattctacag
     1861 tggaccaaga ttagttccaa ttgttagttt aatagttatg agtatagttg gattattaat
     1921 acctattgtt tggccaccat ttttcaatgc attccaaagt ttaggaagat ggatttcttc
     1981 agcaggacca ttaggatatt tttcatatgc agtggcagaa agagttacaa ttccatttgg
     2041 actaaatcat cttgtaacat ctgtattccg ttttacacca ataggtggaa ctgctgttat
     2101 tgatggagaa acgtactttg gtacattgaa catgtttatg gcatatgtag aaaataatca
     2161 gattatacct cttgatttag ctggtaaaat ggaacaaggt aaattgatga ttcaatatgg
     2221 attagctggt gctgcattgg ctatctaccg tacagcaaaa ccagaaaata gaaaggcact
     2281 taaaggactt ttaatatcag gagtgttgac agttattata ggtggtataa gtgaaccaat
     2341 agagtttcta ttcttattca tatcaccagc attatttgct ttccatacat ttatgaatgg
     2401 tatagcaaac atggtacttc catacttagg cgtgttgatg ggattcacag gagatttaat
     2461 tgcatttata agttttggtg tgttaagagg aacagctaca ggatggccaa tagcagtatt
     2521 agtagcagtt ttatattttg caatatacta ttttgtattt aagtgggcaa ttattaagtt
     2581 taatataaag accccaggac gtgaggataa agaaataaaa gtatctgaag gaaatggttc
     2641 agatttcaaa aatttgtcta catataaagg acaacaaatg atatctgctt taggtggtca
     2701 atctaatata ttatcactag ataattgtgt aactagattg cgtttaaaac tacaagatgt
     2761 atcattaata aatgaggatg caattaaaga ggctggtggt atagcagttg ttaaattaga
     2821 tgagcataca atacaagtaa ttataggaac tcaagtgtat tctttaagaa agcagatgga
     2881 taaagctatg gaaggtcatt tggaggcatg taatgattaa ttttgataaa aaagtagacc
     2941 gattgggtac atattgtacc cagtgggact atgtagaaga tagatttggt aagaaggatt
     3001 tgcttccttt tacaatttca gatatggatt tagagtttcc atttgaaata acagaggtgt
     3061 tgcaggaaag gttaaatcat agagttttgg gatacagtag atggaaacat gatgatttta
     3121 gaaattcagt tacgaattgg tatattaaaa gatttaattg tagcgttgat agtaactgga
     3181 tatattatag tccaagtgta atgtattcaa ttagcaaact tattgagatg ttaagtaatg
     3241 aaggagatgg aatccttata aatactcctg catacaatgc tttttatgat gtgataataa
     3301 ataacaatcg taatattata aagtcacatc ttgtaaatag gaatggattc tattcaattg
     3361 attttaatga ttttgagaaa aagtgtaaaa aatctaagat atttatttta tgtaatcctc
     3421 acaatccaac tggtagagta tggagtgagt tagaacttgt taagatgata ggaatttgta
     3481 agaataatga tgtcaagata atatcagatg atatacacat ggatattgta tatgacaata
     3541 gaatgacacc tatcttaaat gttgcaaagg actatttaga ttctgtattc atttgtacat
     3601 cagcgtctaa atctttcaat ataccagcat tgactgggtc atatgtgatt ataccaaatg
     3661 aaggtattag aaataaattt gaaaatatca ctagataccg tgattttgta aattcgccag
     3721 caattctagc aatattagct acaatggtct gttatgatga ctgcgaatat tggttagatg
     3781 aactattaaa ataccttaaa gagaatctta aatatacgat tgattatata aaagataatt
     3841 tagaaccatt gaaattggtc atgccagaag gctgttactt tgcttggata gatttttccg
     3901 aacttaaaat aagtggtgag aaattccaaa aattattgat ggatattgga gaagttgcta
     3961 ttatgtcggg gtctgtatat ggtgaagaag gtgagtttta tttaaggtta aatgtggcat
     4021 gttcaagaga aaaacttgaa gatggtttaa ataggattaa aaaaactatg gattatattg
     4081 caaaaagaat tttatagtag gaagggagat agttatggga tatttaaaaa caatggttga
     4141 tgttatgact actgaaatga atgccataag atgtttaata gatgaagcag gaatagaata
     4201 tgaaagtata gttagtgaaa ttgcaaattg tgaaggcaaa gtaattttta tgggtgtagg
     4261 aaaatctgca catataggta agaaattggc agctacattt gctagtactg gtacacctag
     4321 cttttttgta catgcaacag aggcagtgca tggagattta ggaatgattg aaagtaagga
     4381 tattacaatt ttaatatcaa atagtggaaa ttctatggaa gtggtaaact gtataaaata
     4441 tattaaagca ataggttcta agacaattgc atttacttca aacagaaatt cagtactggc
     4501 aaaagagtgt gattatgctt taatatatcc tgcaaaggat gaagcagacc atttaaatct
     4561 agcaccaaca acatcatcta caattacttt ggttttagga gattctattg catgtgcatt
     4621 atctaagagt agtaattttg gttcttcaga tttttataag taccatccag gaggaagttt
     4681 aggtgagaag ttaaaaacag ctaacaatgg ataaaagcaa aaaggagagt agtttttatg
     4741 ttcaaaatgt ttaaaaaaaa atcaataaaa tcacccatat caggaaaagt aatagagtta
     4801 tctaaaatac aagatggtgt attttctcaa aaaattatgg gagagggtgt agctatagat
     4861 tctacaggag acattgtata tgctccaatc aatggaaaga tagtagtagt agcagagact
     4921 aaacatgcat ttgtaataga attagaaaat gggatggaac ttcttattca tgttggttta
     4981 gatactgtaa atttaaaagg cgaaggtttt gaggtattgg tgagtatggg agaacaagtg
     5041 aaggaaggaa caccaatgtt gaaaattaac cgtcctttga ttgaatcaaa gggtatatca
     5101 cttattactc cagttacaat aactaatcat tcagagtaca atatgaatac atgtaatgta
     5161 ggcaatagtg ttgaaggtgg gaaagatact gtaattgagt ttaaataaag aactaaaata
     5221 caaggttaat atttataaat acctataaac ttatgtttta taagattgat tgtatagatt
     5281 tgttgtatag atttgttgta taaaaaagaa ctctttaatt ataaattaag gagttctttt
     5341 ttattttgta aatattgttt a
//
LOCUS       DAMJWS010000143         5355 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMJWS010000143 DAMJWS010000000
VERSION     DAMJWS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.148"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(349..549)
                     /locus_tag="PC399_003565"
     CDS             complement(349..549)
                     /locus_tag="PC399_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2594370.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(774..950)
                     /locus_tag="PC399_003566"
     CDS             complement(774..950)
                     /locus_tag="PC399_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594371.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1004..1093)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1273..1473)
                     /locus_tag="PC399_003567"
     CDS             complement(1273..1473)
                     /locus_tag="PC399_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594372.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1475..1825)
                     /locus_tag="PC399_003568"
     CDS             complement(1475..1825)
                     /locus_tag="PC399_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF2594373.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     SKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1987..2176
                     /locus_tag="PC399_003569"
                     /pseudo
     CDS             1987..2176
                     /locus_tag="PC399_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2653..2823)
                     /locus_tag="PC399_003570"
     CDS             complement(2653..2823)
                     /locus_tag="PC399_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594374.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESGYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3212..3706)
                     /locus_tag="PC399_003571"
     CDS             complement(3212..3706)
                     /locus_tag="PC399_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2594375.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKFLND
                     KSNT"
     gene            4511..4888
                     /locus_tag="PC399_003572"
     CDS             4511..4888
                     /locus_tag="PC399_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2594376.1"
                     /translation="MKEEILIEKLLRVELIVMEYLWKKDTLMPKKKIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIINFQSYYKIIIDNKEYYTFKKKVLKSSKSIKIMHSLT
                     TIHKNVSKEKLDLLDEYYRNLKE"
     gene            4993..5355
                     /locus_tag="PC399_003573"
                     /pseudo
     CDS             4993..5355
                     /locus_tag="PC399_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2158 a          639 c          617 g         1941 t
ORIGIN      
        1 gaacattgaa acatatagat atgttttaat tgctttttat taacaaatta ctaaaagtat
       61 tatatcagaa ttgaatacaa aagaaagagt atgtatttat gagatagttg aaaattttga
      121 tgtgccacaa ctaaaattat tatattacta tatattctaa ttaaaagagt acttgtaggt
      181 ttaagtacat actttataaa tatatttttg tcagatttat tttataaaaa gtatttagac
      241 taagtattat ttttaagtat ttttttgttg aagttatttt ttcctttttt acaaaatgcg
      301 taaataaaaa aaagactctt aaaagagtct ttccgaaaaa tctatatatt ataatatagt
      361 tatattttct gcttgaggac ctctagcgcc tttaactata tcaaagctta ctttttgacc
      421 ttcttctaat gatttaaatc ctgaagtttg tatagctgag aaatgagcaa atacgtcatc
      481 tccactttcc attgatataa atccaaatcc tttttcatta ttaaaccatt ttactattcc
      541 attattcatt aaaaatactc ctattaacct atatcaagat atacttgatt tacttgtgta
      601 ttaccacaaa tactattgta tcatagatta acataaatgt ctatttaatt ataattttta
      661 gttaacattt ttatttattc ttaatatgaa aataaattta aacattttat attaccaata
      721 tattaatgta aatgtagata aaactaaaaa gggttttcta gaaaaaaatc tgtctaatta
      781 tacttactta ccaataccat cacttgatgc aagcaagatt ccacaacttt ttctccgtac
      841 tctaatataa aatcatctaa ctgtgtatct ataaaatcac aataatcata tgaactacca
      901 atataagcag ttaacatatg tgtacattta tccattttat ctctaaacat aatgtattta
      961 tccccctaaa aaactttcat gaaaaacaaa aagaaaatat ctttttggca actggctgac
     1021 ataacttatt ttataagcgt tagtccctat agctttgcgt cactaaattt ctctagtttt
     1081 gccgatttag ttttattcta cagattaata atacaataga attagttatt attcaacata
     1141 tctagctgaa aaattaataa atttatatat gaacgttaaa ttatctgaaa aaataaaaag
     1201 cccctctgtc aggagtgact taaaaattga atttaagttt gtatttaaaa cacattttta
     1261 ataatgcaaa aactaatcaa aaataaacaa ttcatcaata ggtgtatcta ctatttttga
     1321 tatatctatt gctaatttca aagaaggatt atactttcct gcttctaaac gcataatggt
     1381 ttctcttcta actcctactt tttctgctaa ttgttcttga gttaattctt ttaattgtct
     1441 atattttttt aggttacaag tgaatttagg catattattt ttcctctaaa atataaagtt
     1501 ttatagcata aacatttaaa gtgattgcat atgtaattga tagtaaaaca cacattaatt
     1561 ctatattcct agacactata gtaatacacc ataaaatgaa taattctata atagcaagct
     1621 gtcctgtaaa tgccatagct gctttttcat cttgtacata cctttcatct gctttatcac
     1681 catttatttt tgatataata aagtttgaga aaaaagcaaa aaatccaata tatgtaaggt
     1741 cagcaacatt gccagatgaa aaatatctaa gacttaaaaa tccaaggaaa ccacaaaaac
     1801 ccataaaaaa ttttttctga atcatttttt atcctcctta gtaattttaa aagagatata
     1861 tttatatctt tatgatacaa atatatctct tttatattct tttgtcaata gaaaatcagt
     1921 attatacaat atttgtactg caccataatt gttagagcaa aaatctactg cttgagatgt
     1981 ttatgtatga ctaaatatga ttttaatttc aaattaaaag tagctaaatt ttatttgaat
     2041 agagaaggaa gagatatttt aatagtaaaa caatatggag tttataacca ttctcaagtt
     2101 gaaagatggg ttaatatatt aggagagtgt gcactaaaaa taaaaaaaat tttctatact
     2161 atatggttca agttagatgt tataaactat atgatgataa ctaaatatag tgtacaataa
     2221 atcgctaagt attttggaat aaacaatagc acagttatag caacatgaaa ataaaaattt
     2281 ttacatagtg gtgttgatgc cttttctaaa atgaaaggaa gaccataatg aataaaaaca
     2341 aaaacactaa agaaaaaata aattaacaag agaacaagaa ttagaatatg aaaataaatt
     2401 tttaaaagat gagcttgagt ttttaaaaaa gatgtgtgcc tcagggaagc cacattaaaa
     2461 aacagtggta tcattattaa tcaaaagaaa gttagatgta ttatcaaaaa attagaactt
     2521 aaatgcatag cattttttca taaatcacat aaatataact cctataaatg tataattgga
     2581 aaagtaaaaa ataataaaac aaatagaaga tttaaaatat caatagcata tcagaaaata
     2641 acaaatgata tgctagaatt tataaaatat atctaatgat aaaaatggaa aatcaattat
     2701 aaaaaaggca tatcccgatt cattttttgt tatatagtca aataagaata ctatagatga
     2761 aagatacgat tttgcatatg tattaagtaa gtattctgat gtttttggtt gatacacatg
     2821 caataaagaa gttgtgtttg cagataaatt agcaaaacta tagtataagt tgcaacaata
     2881 aagaaaatga aaattatcat actaaagcat aaggcatgtt acaattttat tagataggac
     2941 attaacatac attaaagtac tttttattat aaagagtact tatttttttg aaattcatta
     3001 atgtataaac tatcaagaat attactcaac ataatttaaa aacaacttga ataggtattt
     3061 ttaaattatg tgatataata aaaaatagaa gtgtatgcca atacacttct gtaattataa
     3121 ctaagcattc tattcttgag gagtgctttt ttcatttctc caaacaagat tgtttatttc
     3181 atcagcaatt attaaataaa atttattgta tttaagtatt cgatttatca tttaaaaatt
     3241 ttattacaat atctattttg ttttgctcgt tcattatgtc tattgaactt ccataatcac
     3301 tttttaattt atctagacca cttttgctta tttctccttc tggaagtata cttaattcta
     3361 gatatttttc atgaccagga gatgacatta atagatatga accattataa ggcataccta
     3421 aattttctat tttatctgct aatgggtcat ctttaaagcc accatttatt attatttttc
     3481 catctttctt tacccatgat atcattcctc catcagtata ttttgattca tctgcatagt
     3541 agctaagaga cgcacttttt attgcactta tatcagcttc aagtttagct atctttgctt
     3601 tttctatatt tttaaataat gctggaagtg ctactattgc caatattcct attatagcaa
     3661 ttactactaa taattccact agagtaaatc cttttttatt tttcatttat ttcccctcca
     3721 tataaaatta ataatatata aatttttatt ttgttgattt ctactttcta tattttacca
     3781 tatatagaca aaaaaatcac tttctatgac aaatatacta ttattttaca atttatggaa
     3841 atgatttaaa atgacatttc ttgttagaaa agatataaga aatggattaa aataaagaaa
     3901 actacagtca tatcaatgtc aataatatta tttaaaagca gatgaaaata aaaagaggac
     3961 tcagaaaata atgtctattt aattatactt acttaccaat accataactt gatataaaca
     4021 agattatact acattttttt tatgctctag tataaaatca tctaactgtg tatctataaa
     4081 atcaaaataa ttatatgaac tatcagtata ataagtcaaa atatgtatac atgaatacat
     4141 attttaaaag atatatatta ttacatacct aaaaaacttt cataaaaaca aaaataaaat
     4201 atgcttttag taacttgtta acataactta taatttattt tataggcatt agcctatata
     4261 gctttgtgct actaaatttc tttgattcta gcgatttagt gctattctac aactaaaata
     4321 atacaataga gctatttatt gtttggtagc attgtttgca aattaaacaa atttatatat
     4381 atgaatatta ttttacgttt aaaaaaacag actaaatatg tagaatattt ggtaaaactt
     4441 acctctagtt tttgtcttaa aaagctccta ttattaattt aagttagaag aattaaggaa
     4501 gtgacataaa ttgaaagaag aaatattaat agaaaaatta ttaagagtag aattaatagt
     4561 aatggagtat ttatggaaaa aagacactct aatgcctaaa aaaaaaattg taaaagaaat
     4621 aaaacagatt tatggatggc ataaaagtac aataaaaata ctactaaaaa ggctagttga
     4681 taaaaggtat ttggctagat atattataaa ttttcaatct tattataaaa taataataga
     4741 taataaagaa tattatactt ttaagaaaaa ggtactaaaa tctagtaaaa gcataaaaat
     4801 aatgcattca cttaccacta tacataaaaa tgtaagtaaa gagaagttag acctattaga
     4861 tgagtattat agaaacttaa aagaatgata tagtaaaaat cgaatgtaat tatttacctt
     4921 aattcctttg tttgtaaaac tttattgata tagaatttga catatacata aattggtaaa
     4981 gggatgtata ctatgaaaat tagtaaatta ccagaagcgg aattaaaagt tatgagatat
     5041 atttgggaat cagaaaaggt attaatacca agagaaatca ccaagtctat ggaacaaaaa
     5101 tatgaatgga aagatacaac tatatttaca gtattaaaaa gattactaaa gaagagagtt
     5161 tttagaagcg gagaaaatag ataagtgtac acactataat gtattagtaa aaaaaaataa
     5221 tatcagagat tttaaacaaa agaattttta aagaatacac ataaaaattc aatattaagt
     5281 ttaatgtcag cactatgtag taaggatgat gaagtagatg gtgatatttt aaatttatta
     5341 gaagaatatt ttaag
//
LOCUS       DAMJWS010000144         5336 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMJWS010000144 DAMJWS010000000
VERSION     DAMJWS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.149"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            217..1680
                     /locus_tag="PC399_003574"
     CDS             217..1680
                     /locus_tag="PC399_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2594377.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1736..2872
                     /locus_tag="PC399_003575"
     CDS             1736..2872
                     /locus_tag="PC399_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2594378.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3108..4223
                     /locus_tag="PC399_003576"
     CDS             3108..4223
                     /locus_tag="PC399_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF2594379.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4375..5244
                     /locus_tag="PC399_003577"
     CDS             4375..5244
                     /locus_tag="PC399_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2594380.1"
                     /translation="MDYLKYFKKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         2052 a          545 c         1002 g         1737 t
ORIGIN      
        1 caaacacacg tcgttaaaaa ttttataaat ttatgtttag atatgattaa tactcttgat
       61 tttagaaata aatatttgta gattgtagat taaagataat ataaataaat agaaggtaga
      121 ttttttagat atattaatat aaagattata taaaatattg aaatatagct gtttaaatta
      181 ttgtctataa atataaaatg aatggtggtg agttaagtga ccgatttagt taacaccatt
      241 aatggaattg tttggtcacc cgtattagtt gcaatgtgtt tgtgtatagg attatatttt
      301 tcattgaaat taaaattttt tcaaataaga gatgtaaaag aaatgtttac tctcttgctt
      361 gaaggagata gttctcatgt tggtatatca tcatttcaag gttttgctac atctatggct
      421 ggaagaatag ggactggaaa tatagctggt gtagctgtag caatagcaat gggaggtcca
      481 ggagcattag tatggatgtg tataatggct acattaggtt cagcaactgc atttgtagaa
      541 tctactctag ctcaaatata taaagatgag catgatgggc agtatagagg aggacctcca
      601 tattattttg aaaaaggtct tgggtggaaa aagtgttcag ttttattttc aatagttgca
      661 ataataagtt atacattttt tatgcctggt acacaatcaa acactttata tctagcaatg
      721 aatcaagcat ttggattttc aaaaatgaca atagcagtag catcttctat tgcattagcg
      781 ataataattt ttggaggaat taaaaggatt ggtttatttg ctgaaaaagt agtacctata
      841 atgggtggag cataccttat tatgacatta gtaataatta taacaaatat tagtttagtt
      901 ccagaggcta ttgtaacaat gtttaaatct gcatttggat ttggttcagt ttttggagga
      961 atgctaggtt cagcaatatc ttggggagta aaaagaggta tatactcaaa tgaagcagga
     1021 gaaggttctg ggacttatgg tgcggcagca gcagaagtga gtcatccagc caaacaagga
     1081 ttagttcaag cattttctgt ttatgtagac acattattaa tatgtatggc tacaggtatt
     1141 atgattgtaa taacaggaat gtataatgtt acaggtgttt gcacagcact gccaggtgta
     1201 gaaccgggac cacaatatgt acagagtgca ataaatacat tacatccaca attaggtgat
     1261 atttttatag caatagtttt attattattt acatttacaa ctttattagc cttctcatat
     1321 atgatggaga ctaatgtatc ttacttagta actagaatta aagcagaaaa acatgaaaag
     1381 tacataatta atgtctgtag atttatgctt atagcatgtg cagcaatgtg ttgttttata
     1441 gaaccactta caagctgggc attaggtgat atatgtatag gtgtattaac atatataaat
     1501 ttaattgcca tctttacact aaaaaaacca gctataaagg catattatga ttatataaga
     1561 cagaagaaga tgggaataaa aaggagtgat agaactttta accctataga gttagatata
     1621 agaaatgctg agttttggga aaatagatac ttaaaagagg ataagcatga gttaaattaa
     1681 agttataata aaatataaaa attactaaat aaatttagtg ggaggtaaga ttggtatgaa
     1741 agaaagaata atacaagtgg ctgattcaaa gaaagaaaaa attctagggc tatgtcaatc
     1801 tttatatgat gaaccagaaa ttgctttgca agaatataaa tctgctaaaa aaatatcaga
     1861 atttttaaga gaagagggtt ttgatgttga ggaaaacttg gcaggtatgg ctacagcttt
     1921 taaagcaacc aagaaaaatg gagatggtcc taaaatagca tttatagcag aatatgatgc
     1981 tcttccaggc aatggacatg catgtggaca tcatttaatt gcatctatgg gagttggagc
     2041 tggaatagct ctttcaagta tactggatac ttacaaggga gaagtaagta taattggaac
     2101 acctgctgaa gaaactggag atggaaagcc ttacttgata gaacatggtg tatttgatgg
     2161 ctatgatgca gcaatgatga tacatccaaa ttcaaaaact tgtgtaacac ctgaaattat
     2221 agcaatagga ggtctagatt tcatatttac tggaaaagca tctcatgcag gagctaagcc
     2281 atataacgga ataaatgctt tagatgcagt agtattacta tataataata taaatgcact
     2341 aagacaacaa ttagtagatg gaacgagaat acatggtatt atactagaag caggaactgc
     2401 tgcaaatgta atacctgata tgggaaaagt aagattagag ataagggcaa aagaacaaaa
     2461 ttactttgat gaagtagtag aaaaagtgaa aaattgtgct agaggtgctg caatagccac
     2521 tggatgtgaa cttgaatttt atcactttga accaacttgc caaggattaa atgaaaataa
     2581 agttttggta gacatattta caaagattat ggaggaattt ggaatatatg aagatgaaca
     2641 agtaatgtta ggttcaacag atatgggaaa tttaagtcaa ataatgccat gtatacatcc
     2701 tttgatgaag ttttctgaaa atggagaaga actacataca aaagaatttt tggaggcttc
     2761 tataaattca tatgcaaaag atagagtaat tgatggtata aaaatattag ctttaacagg
     2821 atttaattta tttgaaaatc cagagttatt gaaaaaaatg aaggaagagt agaacttaga
     2881 ctatttttaa ttatactctt taaaataaga agagtgtctc aaaatgaact ttttagttta
     2941 tgagacactc ttttttgtat gaaattaaat atatttttat tattttaaca atgaaaaaga
     3001 ggcttatctg tttttatatg acctttttta acttgtagca caaaatatca tgaatgatta
     3061 caattcgaat gataaaatcg attataagat atataggagg ggtacatatg gaaactttag
     3121 aagtaagaaa tattgaattg agtggtacaa attatgagat aggatacaga ctaggagaat
     3181 tggttgctaa tatgccagag ataatagaag gtcagattaa taaatctaat ctggtaagca
     3241 aaaaagaaga aaaagagatg atagagttgt ttgataaata ttgtccaggt cttaatgaag
     3301 aattgcaagg ttttgctgat gcaattcaag tgaattgtaa tcaaatttta tattatacta
     3361 tgacttatct taaaccaggt tgtagtcaag ttgcattagc accagaatta actgaaaatg
     3421 ggcatgtact atttgcacgt aatttcgatt tctcacacaa tatggaggat tttgttttat
     3481 gtaaaactaa ggtaaatgga aaatatgcac atataggtac taccgttatg caatttggaa
     3541 gaggtgaagg tatgaatgaa tgtggcttag gtgtaagtca gtcatcatgt ggtattcctg
     3601 taggcaattc agatggttta agaaaaccag caattgtagg tttacagttt tgggctgtta
     3661 ttagatattt gttagaaaat tgcaaagatg tagatgaagc attagaatat ttaaaagata
     3721 tgccaattgc atacaatatt aatttattgc ttgcagataa gtctggaaac attgcacttg
     3781 tggagacttt agatgggaaa aaagaagtaa atataatcaa tagtttagaa agtaaaagag
     3841 aatatttttt acactctact aatcatacac atattgataa acttcataaa ttagagccac
     3901 aatcaatgaa aaattctatt catagatata aattaattaa agaatatata aataagtcta
     3961 aaaaaattgg agagaaagag ttaatgaatt tattgtcttc aaaatatcca aatggcttat
     4021 cttgtaatta ctataatgat ttttttggaa ctttaaaaag tatagttatg gatttaaata
     4081 taggaaagtt taatatttta tggggtggac tagagaataa atgggagagc tactatttaa
     4141 aaaatgacat taaatctata acacagagga taaacataaa tattgaaaaa gcaccatctg
     4201 acttttttga tttcattgaa taaaaaatgt atttaattat tctaaataat taggatatat
     4261 aaatcaacta aaagttttta tttatcgtat tgaattagtt ataatatttt agtatagtaa
     4321 aaaatagtgc tattttacat atttaaagag tttaagtaga agagaggtgc catattggat
     4381 tacttaaagt attttaaaaa agctgtaatg tatattgaga ataatttaca tgaaaatata
     4441 actgtaaatg atgtggcaaa agaaactgga tattcttatt atcatttaac acgcttattt
     4501 aagtctatgt ttggtgagag tgtaggaagt tatataaaaa aaagacgatt agtaagtagt
     4561 acaaaagaac ttttatatag tgataaaaaa gtgatagata tagctattgg tagtggattt
     4621 gaatcttcgg aagcatttag cagagcattt aaatctatat acaaggttag ccctatagaa
     4681 tatagaaaaa atagaattga tgtatttgtt ggtaaaaaga aaaaattaga gttagatttt
     4741 atgagacatc ttgttggaaa tataacgatt aaaccagtta taaaagaaat agaaaaaata
     4801 aaggtaatag gtataaagga taaagtaatt ttagaaaata attcattacc tgacttgtgg
     4861 gagaaattca gaaaagtaca tcatattgtc ccaaatactt tgccatcaaa gagagttttt
     4921 ggaatctgtg aagcaatctc tgaaatacac ctagttagtg aaagtatgga atttaatgag
     4981 ataataggac ttgaagttaa tagttatgat gtgattccaa attcatttgt ttcaaaaact
     5041 attaaggaag gaaagtatgc tatatttact catactggta gcttagataa tttagacaaa
     5101 acttatgaat atatatgggg tacttggttt ttaagttcaa aagaagaact tgatataaga
     5161 gatgattttg aagtatatga tgaacgattt ttaggtccta gtaatgtgaa ctcacaaatt
     5221 gatatttata tacctataaa ataatctata agaaaactaa aaaataattt gattaagaaa
     5281 tagatgttcg taaaagagca tctatttttt atttttatca gatttagata atttta
//
LOCUS       DAMJWS010000145         5179 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMJWS010000145 DAMJWS010000000
VERSION     DAMJWS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.150"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            56..1828
                     /locus_tag="PC399_003578"
     CDS             56..1828
                     /locus_tag="PC399_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2594381.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            1850..3271
                     /gene="ccpM"
                     /locus_tag="PC399_003579"
     CDS             1850..3271
                     /gene="ccpM"
                     /locus_tag="PC399_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HDF2594382.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3300..4370
                     /locus_tag="PC399_003580"
     CDS             3300..4370
                     /locus_tag="PC399_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HDF2594383.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4386..4637
                     /locus_tag="PC399_003581"
     CDS             4386..4637
                     /locus_tag="PC399_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HDF2594384.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4688..4891
                     /locus_tag="PC399_003582"
     CDS             4688..4891
                     /locus_tag="PC399_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594385.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2108 a          455 c          830 g         1786 t
ORIGIN      
        1 ttatattaat taataaaata ttttatttca attttattaa gtatggaggt gtattgtgga
       61 aaatggtaaa tttcaatttt ttaaaggtgt aagaaattgt tttaatatga tttatctaca
      121 tgataaaaga tatttattta tctctatgtt tatggcaatt tttcagggga ttgttccatc
      181 tatttctttg ttagtaagtc aaaatatcat taataatatg caaacatttt cgaaaagtgt
      241 aaaagaaata ttagttttgt taattatgta tttaatcata aatataattc cttcaatttt
      301 tcaaagtttt tatggatatt atagaataaa gtttaataaa aattttgaaa aacatattga
      361 aacaatgatg ttagagaaat ctttaacttt gagtttaaaa gattttgagg atgataatac
      421 ttatgacatg ataaatagag ctcaatctca aaaaggattt aatattacta tactatctga
      481 aaaccttatt tcaataataa gagaatcagt atctacactt tctcttattg ttgtattatc
      541 acaatttcaa ctttggctaa taatagttgt tattgttatt cctgtattga aatcattata
      601 ttccattaaa ttaggaaagg aacaatatga tgttagtgta aacagaactt ggaaagaaag
      661 aaaggcctgg tacatagatt atttgttgtc aaaaggttat gcttacaaag agattatggt
      721 atttagaatt ggttctaaac ttattaatca ttataaaaaa ttaaaagagg atattatatc
      781 tcaagatatg aatatagcta aaaagaattt tattgctacg ctattatgta cagttttaga
      841 ttcaattgta tctgcaggaa ttataggatt tataataatt caaggtattt taaagaagat
      901 tttaatagga gatgcgacta catatataca agcagttcaa tctataaagg ataatgttga
      961 atcaatattt tcgttattta gttcagtaat acaagatctt ttttatacag atttactgtt
     1021 taaatttcta gattataact gtaaagaaga ctcaattgaa catataaaaa tatttgaaaa
     1081 aataggggat ataaataaaa tagagttaat aaatatttca tataaataca atcattcatc
     1141 agagtattct ttaaaaaata ttaatttaac tttaaataaa aatgatccca ttgctatact
     1201 aggaaaaaat ggttcaggta aaacgacact tttaaaaata ttattaggtt tttatgatga
     1261 ttatgaaggt gacatcttga taaatggaaa aaatttaaag gatattgaaa aagatgaata
     1321 cattagaaaa gtaagttgta tgtttcaaga ttatattaaa tatgaggcta cattaaaaga
     1381 aaatgtcgcc atgggagatg tatataagag taacttggaa gaaaaagtta aaagtaaact
     1441 taaagaaact aaattgaaac caagtattta tgaggaagat ggattagaaa ctattttagg
     1501 aacctggttt ggtaagaagc aactttctat gggtgaatgg caaaaagtag caatatctag
     1561 aactcttatg aaagatgctg atatgtacat ttttgatgaa ccagattcat ctttagattc
     1621 tgaagcggga aatgaactcc tttctttata taaaaaaaca tttgaaaata agattggaat
     1681 atttataacg caccatatcc aacatgcaaa aaagataact aacaatattg tagttcttga
     1741 aaatggaaag ttgttagaaa gtggaagaca tgatgaactt atggacaaaa agggtgttta
     1801 ttttaattta ttttgtaact gcaaataaat attaaaggga ggttaattaa tgaatcaaaa
     1861 accatttata cacacattta aagctggaaa atcatatttt atttatgatg ttaatacgga
     1921 taagatttta aaggtaaatg ctgctgttta taattatcta aataaaattg agcataattt
     1981 agaaaaagaa agtgactgga acatagaaat agaagacgaa attaatacct tgattaatta
     2041 tggatttcta aaaaataaaa gggtaagtaa aacaatgcac cctgaaacta agtatataaa
     2101 atatcatata gaaaataatc tatcctctat tattttacaa gtaacccaaa attgtaattt
     2161 acgttgtgac tattgcattt attctggttc atacagcaac agagttcata caaataaaag
     2221 aatgaatata gatgtagcaa aaagaagtat tgactatttg attaagaatt ctagtcaatt
     2281 aaatgaagtt agtgttggct tttatggagg agaaccctta cttgagtttg atttgattaa
     2341 gcagatagta gaatatgtaa aagtagttgg agaaggaaag gaaatatact ttaatctaac
     2401 tacaaatgca actttattta atgaggaaat aatagaattc tttgtaaaaa ataatataac
     2461 aactatgatt agtttagatg gaggaaaaga aattcatgat aaaagtagaa aatttgcaaa
     2521 tacaggagaa ggatctcatg atgttgtatt aaaaaatgta cgttacataa aaaataaata
     2581 tccagaatat tataaaaaga atattacatt taatacagtg ataaattcag aaaatgatta
     2641 catttctata gatgatttta tatctaaaaa tgagacttta aaagattcaa actttttagc
     2701 ttccactctt aataataact attctgataa agaaatagtt tcatcagatg aatatatatt
     2761 aggtaagagt tatgatagat tcttgacttt actcaacaaa ttaaaattta taaaagatga
     2821 gagtatgtca cctttatcaa gaatagaaag agccttatta gatgatttta gatatgagaa
     2881 aaatattgaa ggtagattag agttaccaga tgtgtggcat catggtggac catgtatacc
     2941 aggagctttt agaatatttg taactacaga tggagaattt tttccatgtg agagagtaag
     3001 tgaaagttct aaaatgggta tgattggaaa tttagatagt ggagtagata tagaaaaggc
     3061 tgaaaaactt ttaaatatag gaaaattata tgaagagcaa tgttctgatt gttgggccta
     3121 tagttattgt gatatatgta tagctaagat tgatgatact aaatgtaatg aaaatgaaat
     3181 aaaaccacat gattgtgaca gaattagaca ttctacagaa aataatatga aagattactg
     3241 cgttttgaaa tctctaggat atcaatatta aattttctaa tgaaaggaaa gatgggatta
     3301 tggaaaaagt acttatatat ccatttaatt ataatttttt tactatttta aatcattgca
     3361 ataaaatttc agatatagaa attaattcac tggttagtcc ttctggctgg gggttatgca
     3421 ataaggagta taatgttaat aatattatta ctaaagttac aaatgatttt caaagtgaac
     3481 ttaaaaagaa ttctttggta tggtttgttg attcaaaaga aaatctagac ttagaaaaat
     3541 ttataattcc taaattaaaa gtagctctaa aagaaggtaa aaagatatta tatacacgtg
     3601 atgatcttaa taaaatatct aggtatatat ctcattatga attcaaaaag attaatgaat
     3661 ggaatttaga aaaatatgaa ataagagata tagagggtat atcacagata aacacaccaa
     3721 taatatttat ttgtggtctt tatggagggt tagatacttt tgatgtaaat ataaggatag
     3781 gagaagaact aagaaaaagg gggttttcaa tattacaatt tggccctaaa aaagagagct
     3841 atttatttaa aatgattcct ataccagagt ttatgtttga tgattcgata agtagtaaga
     3901 caaagattat taattttaac cattttataa aagataaaga ggtagaggca aagccagatt
     3961 taataattat agaagttcca ggagaattat tccctttctc tagaaaaatg gttggtaatt
     4021 ttggaattag agcttttgaa atttctaatg cagtaataaa tgattgtggt atattatgta
     4081 ttccatcaag tatatatgat agagaatatt gtgaagaact taaatatttt attaaaggaa
     4141 gacatggtat aaatatagac tattttagta tagagaaaaa agttatagat acatatgaga
     4201 cagaggtgaa gcaaaaattt tcatatatgt cattagatga aaactttttt gttgaagata
     4261 tagaagatta tgaaaaaaca ttttttatac atgataataa acaattacta gaattagtgg
     4321 acaatatagt gaatcaatta aaagagtatg ctaaagtaat atctatttag aattggaggt
     4381 taattatgaa ttacactgaa aaggaattac tactagagaa tatatttttt gatgtgactg
     4441 gaattaattt taataaaaat aacaatttaa aaaaagaatt atttttttca ccgtctttac
     4501 atatacagcc aagagaatta cttttagtat tttgcgaggt aatagaaaac tttaatataa
     4561 aaattccaga ggaaaaactt ttgaataggc aatttacgag ttttgataat gttctaaaaa
     4621 taatcaatat ggtataggtt aataaaggta taaagtaagt gatcttatag aaaaggggag
     4681 gatatgaatg agttttggaa aaagaataga aatgaataat ataggtgttg agacaagagg
     4741 tgtttgtact attatgtgtc cttatacttg taagatgatg gcatcttcat cttgtaactg
     4801 caagaataat gattataatg caagaggagg atcatataat acatattata ataaattgat
     4861 acatggccaa agaactcctg atggaatgta aatatttcag taataagtat actattaaat
     4921 tgatttagca tatttgatta aatttatctt tcttacttta tttcctttta ttataaaaat
     4981 atgcagttga ttataattaa gagtgtgatt ttataataac tgcataaata gtatctgtta
     5041 tataaatcac ctcttcaatt attatagtgg taaatatagt gattaggggt gatttttaca
     5101 atttaaaagt ataaattaat taaaagttat tataataaat tatacttatt taaatagtaa
     5161 gaattaaaag taaagtttt
//
LOCUS       DAMJWS010000146         4991 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMJWS010000146 DAMJWS010000000
VERSION     DAMJWS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.151"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..324
                     /locus_tag="PC399_003583"
     CDS             <1..324
                     /locus_tag="PC399_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2594386.1"
                     /translation="ICIEDLQVKNMIKNHKLSRLISDVSWSEFIRQLEYKANWYGRQI
                     VKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLI
                     TIQNK"
     gene            451..1620
                     /locus_tag="PC399_003584"
     CDS             451..1620
                     /locus_tag="PC399_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF2594387.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1783..2325)
                     /locus_tag="PC399_003585"
     CDS             complement(1783..2325)
                     /locus_tag="PC399_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF2594388.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2473..2757)
                     /locus_tag="PC399_003586"
     CDS             complement(2473..2757)
                     /locus_tag="PC399_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2594389.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2820..3584)
                     /locus_tag="PC399_003587"
     CDS             complement(2820..3584)
                     /locus_tag="PC399_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2594390.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(3866..4132)
                     /locus_tag="PC399_003588"
     CDS             complement(3866..4132)
                     /locus_tag="PC399_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2594391.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(4174..4983)
                     /locus_tag="PC399_003589"
     CDS             complement(4174..4983)
                     /locus_tag="PC399_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594392.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIEELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         1724 a          682 c          713 g         1872 t
ORIGIN      
        1 atttgcatag aagatttaca agtaaagaat atgattaaaa atcataaact atctcgctta
       61 atttcagatg tatcatggtc agaatttatt cgtcaattag aatacaaggc taattggtat
      121 ggtagacaaa ttgtaaaggt aggtaaattc tttgcaagtt cacaaatatg caataaatgt
      181 gggtataaaa acgaggaagt taagaactta aatataagag aatggatttg tcctagttgt
      241 aatgaaactc acgatagaga tataaatgca agtataaata tattaaaaga aggattaaga
      301 ctaataacaa ttcaaaataa ataacgtatg taagaaccgg aggaactacg gggatagcct
      361 gtggagaatt agtaagacat attttagtat gcaaaattct gtgaagcagg aaccctgtga
      421 ctttagtcat gggaggttca gggtagaaag ataaatgaac tttgtaaaga tgaagttgta
      481 gagttttgca atagaataaa aggtaaagtg gaaagtgttg caataagtgg agtatttgct
      541 ccagtaaata aacaacaaga agaagaagtt gcaaaatggg ttagagaaat tttaggggat
      601 attcctatat ctttatcaaa tgaaataggt aatataggta tattagaaag agaaaatgga
      661 gctattttaa attctgcatt gtctcaagta ggtaaagctg tatctttagg atttgaaaaa
      721 gcacttaaag acttaggtat agatgctcaa atatatttca gtcaaaatga tggaacatta
      781 atgaatttag agtatacaat gaaataccca atttttacaa ttggatgtgg aataacaaat
      841 tctataagag gagcttcctt cttatcaaaa gtaaaaaatg caatagtatt agatgttggt
      901 ggaactacaa gcgacttggg tgtactatat aatggattcc ctagagaatc atctattcca
      961 gttacaattg gaggagtaca tacaaacttt agaatgccag atgttttaac tattggtctt
     1021 gctggtggta catgtgtaaa aggcgaaaaa gataatataa agataggtcc tgaaagtgtg
     1081 ggatatagaa taactgaaga tgcaataata tttggtggag acactctaac tttatcagat
     1141 gttgttacaa aattagatat ggcgtttgaa gatagaaaaa gcaatgttga aaacttagat
     1201 aaagagtatt gtaatgaggt atataagaag gttattttaa aattagaaga tgctgtagac
     1261 caaatgaaga ctagtcaaga tgatgttgaa ttggttttaa ctggtggagg aagtataatt
     1321 gttcctgaaa aactaaaagg cataagcaat gttataagac ctcctcatta tactgctgca
     1381 aatgctattg gtgcagcttt aggacaaata agtggagatg tagaaaaagt atacagttta
     1441 gataaaatgt cgagagaaga tgccatagaa gatgcaaaaa aagaggctgt aaataaagct
     1501 ctaaaagctg gtgctaaaga agatactata gaagtattaa atatagaaga tgttccactt
     1561 gcataccttc ctggtaatgc acttcttata aaagtaaaag ttgttggtga tttaatttaa
     1621 agaataaata taagtatttc cagaattata taaatatttc tggactcatt tttaaactta
     1681 ttttatcaat aaaaacagca taacttttga ctatattctt ataagtttcc gaacagccga
     1741 tatataccta ttttttgagg tttatcggct gtttgttatc ttttatcgca ttccaactat
     1801 ttttcttacc gtatttgctg tacgaattgt aatcttatca cttacatttg aactggcagt
     1861 ctttgaccac caatttgtct ttttataatc tttacgacta aaagaccaaa agatagcatt
     1921 tttataatga tatacttttt catactcttc tttaggattt ccaacctcat cataaaactc
     1981 gtcataagat aagtcaggaa acataaaaat aagattatca tatatttctt tgttgttatc
     2041 tccccaccaa ttaggagcat tatttagtat atcttttaac tcttcttgtg aaataataaa
     2101 aactggtatt tccaattcaa actgtttttt tatcattaat cttattgtat ttgagataac
     2161 cttgctgtca tctacatcac ttaaaaagac aacattacca ctattaaggt atgtagaaac
     2221 ttctgaataa ccaagttcta caaagcttgc ttttaattca gacataatta tcttgttctt
     2281 accacttata ttgatacctc ttaataatgc gatataactt ttcatataat aatttcacct
     2341 ctaaacattc acatttttct atgtagtaca aactggaatt tgcatctttt ataattcttt
     2401 caaaacttct tcatatgtaa tttttttaat ataaaaaaat cggtgcttaa tttgtaccga
     2461 tttttgtttt tcttagtaaa gaactcgttt ttttgcttct gctatagctc tagcagcact
     2521 ttttgcttgc attctttttt cttcttcttt tttaaattca tttattgttt catttagatt
     2581 cttatcatgt tcattttttt ctttatcttc gttttctctg tatactaata tataatctat
     2641 tattgattcg tttattattt gagatatact tttattttca ttaattccta ttctcttaac
     2701 ttccttaagt agagtctcat caatatgaac cgttgtttgc tttgtatttt ttgccacctt
     2761 aatcacctct taaaaaaaat tctaacatta aaattttttt tagtcaataa attttaatat
     2821 taaaattctt taaatgtttt atttgatttt ctaaaatttt ctattgtatt tatcatctta
     2881 ttttttatat tttcacctag tacagaagac tttagtctag gattttcgat atggtcataa
     2941 attggttcta aaaattcgac ttcgacagga acagagttaa ttttaccaat aggttggtat
     3001 ccttcataag tatcttttga attttttatt actagtggaa caatagggca ttttgcttta
     3061 tatgctatct ttaaagctcc acttctaaac tccgaaacca atgaatttgg ttctttaatc
     3121 catgttaaat ctccttctgg aaacactgcc atactttgac ctgtaagtat attttgagag
     3181 gcttgggcta tactctttat accctcacga ttgttttttc tgtttacata tacacatctt
     3241 agtaattttg cccattcttt aaatataggt atatttctcc aaactggttc atctgcaatt
     3301 acacatccaa ttggtctttc tacacttgct gttagtatga agctgtcaag catactagaa
     3361 tgatttacaa caaatagaag tggttctttt ggcagtgttt ccttaccaat tatattaagc
     3421 tctatgttca ctatatctaa agatttttta gcttgttttt gcataaattc aaatttttcc
     3481 tttaagctaa atttatcagg attcttttta attttaataa gcctaggtat agaagatgta
     3541 aaacctataa gttgatatgt agcaaatttt aagtaattat tcataaaatt atctccttaa
     3601 aataaaatta tttattactg taaagatttt aacataaaat aaaatttaag tctattttaa
     3661 ttcaattttg taacatatat tttaaataga cagagattga gattttatat ttccaatttt
     3721 agtaatcaga ttttatcaat aaattactat tctattaatt taattaaata acctttaaat
     3781 ttaattataa aaactgttgc agaaatttct acaacagttt aaaaaattta ccttacaaaa
     3841 acttatttta taacaagaga gtatattata aggctagtac agcccatttt ttgcactgag
     3901 aaattctttt gccatcatca ccttgttctt tgtcatatgc aaattgatta agcatttcac
     3961 atggaaatgt atcacataga ccacaatgtg taaggttttt atcttcacaa caagatttta
     4021 caggacattg ttcaccccaa aatggtttct caatctctgt acaaccttta caattgactt
     4081 tttccttgta tccacattca taacataaaa ttccacatct tgattctatc attttaagtc
     4141 actccttaaa tataaatttt atatgattgt atatcaaaat ttatactttt cattgtaaaa
     4201 atcggacaaa ttttttatat aggttgttgg attaacacca caaatagatt taaaatcatt
     4261 tataaaatga gattggtcgt aataaccaat atcttgtgct aaagttgcaa atggtatttt
     4321 attattttgt atttcttgta atacaaggtt tatacgaagt aatctaaggt atgatttaac
     4381 actcatacca agagaattat taaaaattct atttaaatgt cgttcactat aacaactaat
     4441 ttgtgaaatg tttttaacag acataataga gttttgtttt ctagcatttt caagaattgg
     4501 tatagtcata tctactatat ttgatttcaa taagtatgat aagaataaca tatctaattg
     4561 ttctactaat tcttctatag tacttgttct ttcaaaaatg gaattaacct ctaagctaaa
     4621 taatgtgtta aaatcttcaa gtgaaatttt taaatctgta gattctcttt gagaaagacc
     4681 tgtcaaataa tatactccac ctgggcgaaa ctcaacgaat actctaaaaa gtacattttc
     4741 tatatcattt tttacaatag tagtttttgt tgtggctccc cagaatgcgc tttctatacc
     4801 ttttttgtca aatttaaata tcatacatcc actagcatca ggtattaatg taagattttc
     4861 tttaatactt gtatctggaa tagatattgt atagtgtgct atatactgtc ttaacaattt
     4921 atgtggcaaa atatatgtat actgtttgca ggtatttaaa atgttattga ttattttatt
     4981 cataagtata t
//
LOCUS       DAMJWS010000147         4669 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMJWS010000147 DAMJWS010000000
VERSION     DAMJWS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.152"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(126..929)
                     /gene="proC"
                     /locus_tag="PC399_003590"
     CDS             complement(126..929)
                     /gene="proC"
                     /locus_tag="PC399_003590"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2594393.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1254..3623)
                     /locus_tag="PC399_003591"
     CDS             complement(1254..3623)
                     /locus_tag="PC399_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF2594394.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3628..4536)
                     /locus_tag="PC399_003592"
     CDS             complement(3628..4536)
                     /locus_tag="PC399_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2594395.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT         1369 a          905 c          512 g         1883 t
ORIGIN      
        1 tcattaaatt cctttttata gtaaattact cttattttac ttataccaaa tacatttatg
       61 tatagatata aataagcact aaaaaaacaa ttcaagaaac catatagtaa acttaattac
      121 atctgttatt tactcatatc tttagactta tctatacaag caacaataga ttttataact
      181 gctgctctaa agccttcttc ttctaatacc ttaacagctt ctatagtagt tcctgctgga
      241 gaacaaacca tatcttttaa ttcacctgga tgttttccag tttcaagaac catcttagct
      301 gaccccatta ctgcttgaga tgcaaattta taagcttgtt gtcttggcat tccagctaat
      361 actgctgcat ctgccatagc ttctataaac ataaatacat atgcaggaga agaaccacta
      421 atacctgtaa ctgtatccat taaatattct gatactattt ctgcctcacc aaaagattca
      481 aacagagcct taatcatatt taactcttca tctgttacat tactattttt acaaagagct
      541 gacattcctt cgccaacaag ggctggagta ttaggcatta ctcttactat ttttttatct
      601 gaacctataa aactttccag actttctata gttttacctg ctgctataga tactattatt
      661 ttatttgcat ctattaattc tttaagtgga gttaaagcct tttcgcatat attaggttta
      721 agtgctacta ttaccacatc agaatttgta acagctacct ttgaatcagt tgtagtattt
      781 actccaaatt cttcttttac tttatctaag gcaacttggc ttaaatctga tacagttata
      841 tcttctgcct ttactaatcc tgactgtact atgccaccta tcattgcact tcccatattc
      901 cctgaaccaa taaatcctaa agttttcata tcaaatctcc ttttttctat tagttttatt
      961 ttaaagtagc taaattgact ttattttaac tgcttaaaat tatcttattt ttataaaata
     1021 taaccttatt ataactataa taaattattc ttttgtatgt attacttact aatatgtgta
     1081 tgatttacta aatataaatt taacgatttt ttcattaaac ttaacgagct aacttcctat
     1141 ttagaaagtt agctcataat taatctaaaa ttttaaaaat ggaatcaaac catttatact
     1201 ttattatatc agttacgatt ttatatagta agaagtttta tttacaagca aacctagaat
     1261 gtttgctcag ttcttcctat tatctcatct tgtagtgttc tacttaaatt attgaaatgg
     1321 tcactatatc ctgcaactct aactattaag tctttataat cctgaggatt cttttgagcc
     1381 tctaacagta catttttatc aaatacatta aattgtatat gatgaccatc catattaaag
     1441 tatgctctta ctagattagc catattatct aacccttttt ctccttgaac tactgatggt
     1501 gcaaatcttt gatttaataa tgttccacca gtttttatgt ggtccatctt agcacaagat
     1561 tttataactg ctgtaggtcc attagtatct ccaccttttt caggtgaaat tccctctgaa
     1621 actggttttg cacaaagtct accatttgga cttgctccca taatttctcc aaagtatata
     1681 tgacaagttg ttggtaacat atctactctg tattctccac cacaaacagt tggtctacca
     1741 gttacctcat tgtaatatgc attgaatatc tcttgcatta tttcatcagc ataatcatca
     1801 tcatttccat atttaggagt cttatttgaa actaaattaa gtatagcttc atatccttca
     1861 aaattagcat caagtgcttt aagcaatgta tccatatcga attttttctt atcaaataca
     1921 ttatatttta ttgctgttaa actatctgtt atagttccta taccaacacc ttgtatatat
     1981 ctagtgttgt atcttgctcc acctctttgg taatccttgc ctttttctat acaatcatca
     2041 actattgtag acataagtgg acaaggcatg tgttttgcac atatattttc tatcacagca
     2101 tttccttcta tttttatgtc aatcatataa tgtaattgct ttttaaatgc ttcaaatagt
     2161 tcctcataag attggaaatt tctagggtct ccagtttcaa tacctatttg ttttttacta
     2221 attgggtcat atccattatt taatgttaat tcaagtattt ttggaatgtt catatatcca
     2281 gttagaacat atgcttcttt accaaaacat ccagtttcaa cacaaccact ggttccacca
     2341 agtcttgcat cttctatagt ttttccagct tccataagct cttgaactat tgcttcagta
     2401 ttataaaatg ctggttgacc ccatcctttt cttgatattt cacaagctct ctttaagaat
     2461 ttttgaggag tctttttact tatttgaaca tttgaactag gttgtatcaa tttcatttca
     2521 tccataacat caagtattat ataactaact tcattaacac catcttgacc atctggattt
     2581 attccaccag tgtttatatt tgcaaaatct gtatatgtgc tactttcttt taatgttata
     2641 ccaacttttg gtggtgctgg ctgattgtta aatttaaccc ataaacattc aagtaattct
     2701 tgtgctctat ctctatctag tattccattt tctacatctc tttcatagaa aggatttaga
     2761 tgttggtcaa gtcttcctgg agtaaaagca tcccatatat taagttcagt agtaacccct
     2821 atatgaacaa accaatacat ttgtatagct tgatgataag tttctggttt gtgagctggt
     2881 acaacatcac aagtttcagc aattagtaat aaatcttgtt tcttagcttc atctgtttct
     2941 tcttctgcca tttgtcttgc tttctcagca tatcttttac ctaatataac cattgcatca
     3001 caagctattg ccatagcttc taaatctgct tttttattat atgcatctaa atcattaaga
     3061 aaatctagct cttttaatct tgcttctatg tctttcttta aatctaaaaa tcctttttta
     3121 tatattgtat ctccacaaac tgtatgtcct ggggctcttt gttccataaa ctctgtaaac
     3181 atacctgctt catatgctgc taaccactca ggtgtcattg catttattat cttatctctt
     3241 gtttgtctct ttttccaaaa aggaattatt tcttctttat gtatttttct agcttcttct
     3301 gatacgctaa aatttattat atctctattg tgcattactt ctaaatcttc cattgtatga
     3361 caacaaattt ctggatatgt tggtgctcca ttaggagaat cacctttttc acctactatt
     3421 aattctccat catttatgct aagagttcta ttctcaatat aatgcttaaa tgataaagca
     3481 cgaagtacag gaatctctac acttccctca tattttttat aagcttctgt cataagtact
     3541 gctctttcaa tagatatgtg tggttctgca ttaatacttt cttctcttaa ttttttagtt
     3601 ctctcaaaag ttcctcttgc cattttatta ccctccaatc ttagtattga aattacttgc
     3661 ttcaaacagt ctttttattt cctctaattt ttcttctgat ggtctttgta gttcttctcc
     3721 ttcatacttt ttataaattt tatcatactt gtgtttacca atattatgat atggaagaag
     3781 acttactgct tgtatattca aaggctttaa aaactctatc atttttttaa cctctaaatt
     3841 ttcatcatct acatttactc ctacaattaa tggtattctt atatttatgt tagcccctaa
     3901 ttcacttaaa atcttaacat ttttcagtat taagtcattt gattttcctg taaattttat
     3961 gtgcttatct tcatcaataa gcttaatatc atataaaaac aaatctgcac atttagcaac
     4021 tctttcataa ttttctgact ttgcatagcc acatgtatca attgctacat ggattccctt
     4081 tgatttgcac atgtttatta ctccacaaat gaagtccata tcttgagcca ttacttctcc
     4141 acctgataga gttactccac cacctgattc ttcatagaaa attctatcct tctctatctc
     4201 tttaaccaaa tctcttacag aatactcttg accaacaatc tctcttgcat tgtttacaca
     4261 atagtctaag caagtttcac aaaactcaca cttatcttgg tctaagcata tttttgtttc
     4321 acctttgtaa attgccttat gagggcatat atttatacat gcttcacatt ttgaacatct
     4381 ttcttcatta tacaaaactt gttttgtata tgtttgactc tctggattat gacaccaaac
     4441 acattcaagt ggacaccctt taaaaaatac tgtacttctt atccctggtc catcatgtat
     4501 gctacatttt tgtaagttta taactaatgg attcatgatt tttcacccct aaataatatc
     4561 cattccattt tttcattttt atgcaattca tcttcataga atatacctat gaaaagcatt
     4621 tctacaatta cataatacta tatttgttat tattttgtct atatatttt
//
LOCUS       DAMJWS010000148         4503 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMJWS010000148 DAMJWS010000000
VERSION     DAMJWS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.153"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            34..333
                     /locus_tag="PC399_003593"
     CDS             34..333
                     /locus_tag="PC399_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594396.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            657..2312
                     /gene="ilvD"
                     /locus_tag="PC399_003594"
     CDS             657..2312
                     /gene="ilvD"
                     /locus_tag="PC399_003594"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF2594397.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLNFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2656..3288
                     /locus_tag="PC399_003595"
     CDS             2656..3288
                     /locus_tag="PC399_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2594398.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            3311..4123
                     /locus_tag="PC399_003596"
     CDS             3311..4123
                     /locus_tag="PC399_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2594399.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1747 a          501 c          810 g         1445 t
ORIGIN      
        1 aatagtatat tttggtaaag taggaggaat tttatgaaat ggattaaatg gtatagtatt
       61 acatgcatat gtatttttat agtagtagct ttttatatgt ttatatttcc taataaaata
      121 gaaactattg atacttcatc tgcatatagc tttgttgaaa agaaagtacc aaatagtgct
      181 gtgtatcaag gatacaaaaa gaatccagtt gatggtacta ctacaattta ttacagctat
      241 gataattcaa ctcatatagt tagattatca catccagaag actattcaag gaagattaat
      301 tgggataaag tttcaaacat cagatttgat taatctttaa tatttataaa agtctatata
      361 tctaaggcaa atatattatt aatcttattc atagttgagc actttctatt atagaagtgc
      421 ctcttctgat ttatatcata aaatattact caacaatttg taaaatatat tagtttataa
      481 aatatattga aaagataata ttatataata caattgtgta agatattgat aaaaaatcgt
      541 aataaatttt cattagtata tgctaatatt tttttacaat tttcgtaatt aattaataga
      601 aaaatatatt ataataatat tatgaaaatt taaaaaagta agaaagaggt aatattatga
      661 gaagtgatat aaaaaaagga atagaaggag ctccaaagag agcgttaatg tatggaatgg
      721 gtcttactaa agaagaaatt gaaagaccat taataggtat agtaaatgca caaaatgagg
      781 tgattcctgg tcatctgcat cttgatgaaa tagcagaagc tgcaaaaaat ggagtaagaa
      841 tgagtggtgg tttgccatta gaatttcctg ctataggggt atgtgatggt atagctatgg
      901 ggcatgttgg aatgaattat tccctggctt caagagaatt aatagcagat tcaatagaag
      961 ctatggctat ggcacatggt tttgatgctt tggtacttat acctaattgt gacaagatag
     1021 tgccaggtat gttaatggct gctgcaagac ttaatatacc aagtattgtt gtgagtggag
     1081 ggcctatgct tccaggtaaa aaaaatggaa aagtatatga ttttaactct gcaatggagg
     1141 gtgttggagc atgtaaagat ggaacagtaa gtgaagaaga attagaagcg ttagccatga
     1201 actcttgtcc tggatgtggt tcatgttctg gtcttttcac agcaaatagc atgaattgtt
     1261 taacagaagc tttaggcatg ggtataccat ataatggtac tgctgcgtca cattctggag
     1321 aaagaaaaag gatagcaaaa tatgcaggta tgtatgttat ggagttactt aagaacgaca
     1381 taaaacctag agatatttta acaatagatg cttttaaaaa tgctatagct gtggatatgg
     1441 caatggctgg ttctacaaat acagtacttc acttacctgc aatagcttat gaatcaggaa
     1501 tagagcttaa cttaaatttt tttgatgaaa taagtgaaaa aactccttgt ttaacaaaat
     1561 taagtccaag tggaaaacat catattgaag atttacatat ggcaggagga ataccagcta
     1621 taatgaacga actttcaaag ataaatggaa taaatttaga ttgcaaaacc gtaacaggca
     1681 agactatagg ggaaaatata agaaattgtg aaatagaaaa tgaagaagta atacatacat
     1741 taaagaatcc atatagtaac caaggtgggc ttgcaatatt gaaaggaaat cttgctctaa
     1801 atggagctgt tgtaaaaaag tcagcagttg cagaagaaat gttagttcat gaaggaccag
     1861 caagagtttt taattcagaa gaagaagctg taaatgctat ttttggtaaa aaaataaaca
     1921 aaggtgatgt tatagttata agatatgaag gtccaaaggg tggtccagga atgaaagaaa
     1981 tgctatctcc tacatcagca gttgcaggaa tgggacttga taagcatgta gcacttctta
     2041 ctgatggtcg tttttcagga gcaactagag gagcatctat aggccatatt tctccagaag
     2101 ctatggaagg tggtttaatc gggttagttg aagaaggaga tataatttct ataaacatac
     2161 cagacaaaaa attagagtta aaagtagatg aagttgaaat agaaaataga aaattaaaat
     2221 ttaaaccttt agaaccgaaa ataaagcatg gatacttaag tagatatgct aaactggtaa
     2281 catcagcaaa tacaggagca gttttaaaat agtagactat atttatgtaa attagaaaaa
     2341 gttaagaaaa tattaattgg atatcttagg tgttatagca ctaatgaggt atccttttta
     2401 ttttgtatga aaaacacagc aaaatcttga ttatctttga aaacaataca tctctattat
     2461 aaggaatacg agtacagcat agagatgtat cttttagacc ttgttgaggt aatacaactt
     2521 tttaaaagtt tgatgtggaa tgcttttcgt tccatctgaa ctatgaagaa attgaaagtc
     2581 agttaattct tgacataata aatattaaaa tgtataataa aaaacaaaca gacagtctgt
     2641 ttttaggagg gaaatatgaa tagagaagaa aaaagtaaaa atagtaaaga aaaaattatc
     2701 caatcagcat tttcactatt ttcgtctaaa ggatatgatt caacatctac acaagatatt
     2761 atcaatttat ctggtctatc tagaggtgca atgtatcatc actttaaaac taaagaagat
     2821 atactgagaa gtgtcacaaa agaactttac tcacaaatga ataatttttt agagcatctt
     2881 gttgctgatg acacccttac agcaaatgaa aaaataatag aattggttgt tcatagtgcg
     2941 aatgattaca cacgtagaaa aatggtacat tgtagctggt tagaaaaaat cccattcgct
     3001 ttaatagagg aagttcgtaa tctcaacaat gtagttgcac ccaatattgc taagataatt
     3061 aaacaaggtg ttgaaaataa agaattttct tgtgaatatc cagaggaatt agctgaaatg
     3121 ctcgttttta gtattgatat tttacttgac cctgtattat ttaaacgtga atacagtgaa
     3181 gtatgtaata ggttggattt tttattattc atgttaaaaa aaatggatat tcctctaatt
     3241 gatgagtatg gtattcaaaa atttaaagat ttatttagac aattataata ggaaaagcga
     3301 ggaatagact atgttttata atgcaaaaag ttgtcaaatg atgataggaa gtaatacagt
     3361 gaattatatt gaatttggta gtggtaagaa gacactaatt attttaccag gattaggaga
     3421 tggtttattt cctttacatg gaaaaataca ggcgattgct tttgctttta gatataagca
     3481 atttgcaaaa gattataagg tttatgtatt tagtagaaaa aatcaaatta cagaaaaata
     3541 ttccacaagg gatatggcta aagaccaagc agatataatg aaaaaacttg gaattatgaa
     3601 agcagaagta atgggtgttt ctcaaggtgg aatgatagct caatatctgg caattgatta
     3661 tcctgagtta gttgagaaac ttgtgttggc agttacttct tcaaagcaaa atgatactat
     3721 ccaaaatgtt atttgtagtt ggattgatat ggcgaagaag cagaattaca atgatttaat
     3781 gattgacaca gcaaagaaat cctattctga aagatattta aaaaaatacc aattatttat
     3841 tcctttttta ggaaaagtag gtaaaccaaa ggattttaaa cgatttatta ttcaagcgac
     3901 ctcttgcata gaacataatg ccttttccga attaaataaa ataacatgtc caaccttaat
     3961 tataggaggt gctaatgata aaattgttgg aaataatgct tcttttcatt tagcagaaaa
     4021 gattaaaaaa agcgaaatat ttatctatga gggattggga cacgctacat atgaagaagc
     4081 acaggatttt aatgaaagag ttttagagtt tttaaataag taaacacctt tgttaaaaat
     4141 atacatatga agattggaaa tttaatgtta aaaatagaaa catgaaaata tgcttaactg
     4201 gtatttttac tattcataac caatttttga tacattatct actataaata caaatatagc
     4261 ttcaatgtga ttatatatta gtgtattggt aaagcactta tacaaacaga ggaattttgt
     4321 aaattcatat tatacccaca tttgttaact tatgaaaata taatcaaaat ttttatgaga
     4381 tttttatata aaaaatataa tgtttgtata caaaaatatt gaagatattt tagtaagttt
     4441 tgtatataag caaacatgta ttttttaata tagttatcta aatattattt tatatacaat
     4501 aaa
//
LOCUS       DAMJWS010000149         4502 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMJWS010000149 DAMJWS010000000
VERSION     DAMJWS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.154"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..170)
                     /locus_tag="PC399_003597"
     CDS             complement(<1..170)
                     /locus_tag="PC399_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594400.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTIL"
     gene            complement(167..748)
                     /locus_tag="PC399_003598"
     CDS             complement(167..748)
                     /locus_tag="PC399_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2594401.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            916..1734
                     /locus_tag="PC399_003599"
     CDS             916..1734
                     /locus_tag="PC399_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2594402.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQVFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            2409..3230
                     /locus_tag="PC399_003600"
     CDS             2409..3230
                     /locus_tag="PC399_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF2594403.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGNVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEIWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(3540..4319)
                     /locus_tag="PC399_003601"
     CDS             complement(3540..4319)
                     /locus_tag="PC399_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2594404.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         1663 a          650 c          559 g         1630 t
ORIGIN      
        1 aggattgtgg aaatcaataa aaatatccct gtaaaaatag cactattcat atggaactta
       61 atagaacttg catctgttac cttattattc ttaatccaga acccacaaac catagttgaa
      121 agaaaaagta aaacagtgat aagacttgaa ccaatagtta taattttcat cttacatcaa
      181 catcctctcc aataatattt tctattaaaa tatcaatgaa tttatctcgc tcatatttat
      241 tctttatatt tataccactg tataactgaa atgattctgt agataaagca gaaatctgta
      301 ttggtaaaat aatttgcagg gcaatgattc ttagactcat ttcatctttt ttatttccaa
      361 aaattttacg aagcataggg acaatatcaa gttctgcttg catatctcca ttggttatac
      421 attgattcaa tacaaaagga agcacttttt cataatgtaa tccagtatca cacaattttt
      481 ttaataaatt ttttaaatct tcaataggtc ttagagtata tacactatcg tcatatagct
      541 ctgctattat atttgacatc acatctccta tggcagtact caacaaatta tcctttgttt
      601 taaaatgata atttattaaa ccaactccaa catttgcacg ctcagcaatt tttctaactg
      661 taatctcctc aatatcatct acttcattta aaagattcaa tgtaacttca attatacgtt
      721 ctctagcatc tttatcatta cttttcataa tagacctcca aaacaattaa cattttcggt
      781 tctgaacatg ttcagtataa tatttttttc atcatatgtc aatataattt aattataatt
      841 ttagttgact ctaacattat gatatggttt atattaatat taagctaaaa gtacctttat
      901 aaaggagaca aaaacatgtt taagataggc gatttttcaa agctaagtaa aattagtatt
      961 cgtatgttac gtcattatga tgaaatagga cttttaactc cttctcatac gaataaaact
     1021 aatggatata gatactatag tgcagaccaa ctttctacta caaacagaat ccatgcacta
     1081 aaagatatgg gctttggctt gtattctata aaagaaattt tgacagaata taacgataaa
     1141 gaaagtctca taaaatatct aaatatccac cattctcaag taaaagaaca attagaagat
     1201 acacagaaaa aactattaaa aatagaaacc actattaaaa gaattggagg aaatgatatt
     1261 atgaaaaatt atgatgtaac tataaagaat tttgcaccaa aatatatgat gacacttaga
     1321 agggttatac caacttacca agacgaagga atgttatggc atcaagtatt tttagagaca
     1381 aaagaccaga atgttcaaat tgaaccccca aagtattcta aagcagtttt ttatgatact
     1441 ggatataaag aagattatgt agatgttgaa gtacaagttg ctgtatctgg aaagtataaa
     1501 gatactgaac atgttaaatt taagactgtt ccaagtgtga ctgctgcaac tgcaattgtt
     1561 aatggaaatt tcaatcaagt tgcagacgct tgtgaggcta ttggtaattg gatatcagat
     1621 aataactatg atgtggatgg tcctatgttt aatatttatc atgtaagccc tggcaacgat
     1681 agtaatcctg ataattgggt aacagaggta tgcttcccag ttaaaaagaa gtaattttta
     1741 tattaattgg ataatatttt aatttgttag acaaattact tttagtgtta tgtataaata
     1801 cctacaattt gattaggata ttatcttaat ttgttaaaca aattactttt agtgttatgt
     1861 ataaatatct acaatttaat taggatatta tcttaatttg tcagataaat caattataag
     1921 attatatact atccttatac tctagttaaa tatattttaa tcactaatat aaattagttt
     1981 ttttaacaaa acaataaaag agtaaagtac aaataattcc tttgtgctct actcttttta
     2041 actttcttta gacaataata caatgataaa tataaaaaat tatatattcc attctataga
     2101 aatatgctca ccaataacat gtatgttgtt aatcaatata ttatatcaaa ttatatcaaa
     2161 attacataaa ccttatagga caatcatatc ttattacaag aaaaatgtct tatccatatt
     2221 gaataagtca ttcagttcat atacaaagtc aatttcattg agaaacattt aatatatgtt
     2281 tataaaaatt tcatactatt ctttcatatt tacatgtgat ataatttatt aactcataat
     2341 tttgttaatc agttgtgatt gtagattatt tatataatct aatattttaa gaaggtggtg
     2401 taataacttt gaaattaaaa attgttggta gtggcggtat gtcaattatt ccaagttcat
     2461 tttgcaaatg caaaatatgt gaagaagcca gacaaaaagg cggaagatat gaacgattag
     2521 ggccaagttt atatattgat gatataaaaa tgctgataga cacacctgaa gatattgcag
     2581 tagcttgtaa cagacaaaaa atatcggaag taaaacatat ttcaatttct caccatgacc
     2641 cagaccatgt taagggtatt agaattgtcg aaaaaatagg atgtgacttt atcacaggag
     2701 aaaataatcc aattggcttt tatgcgcttc ctgaagtaat tgaagatata aaccgaacga
     2761 atctcgattg tcttaaatat tatggtaatg ttataaattg catttctatt aatgaaactt
     2821 cgcatgtaaa gataaataat tttgatattg atttgattaa caataaccca gacaggaata
     2881 taaccttcta tgttatcaaa gagaatgaaa aaaaagtcat atatgcctgc tgtaatccca
     2941 agccatttac tcatagtgat atgtactttg atgctgatgt tttaataata agtctagttt
     3001 ctgatgatgg tatattaaat gatggtacaa aacttaaaga cacaccattt aaagatgaaa
     3061 tatttacttt agatgagatt gttgaaatta aaaactatta tagaattaaa aagattatca
     3121 ttacacatat tgacgaaata tggggaaaat catatggtta ttacagtgaa ttagaaaaga
     3181 aacttgataa catttatttt gcatatgatg gtatggaaat tatagtctaa atcattaagt
     3241 aataagcata atgcaaaata agaaaacttc aatttaacag tatagttaaa ctatgtaata
     3301 tcaacatatc tcagttttta caaaatatca tagtatttat ctttataaaa tttcaagtcc
     3361 tacatatttt aatacttcaa tatctaaata aagtaaaatc aaaaatatat tttatataca
     3421 ttatagacgg cttatcggca ctagataagc cgttttgtca caattattat ttgctcaaca
     3481 aaacttctca taatatataa cagattgaat ctccaactga tacttaatta ccgaaactct
     3541 tattccttaa tatgttgttg cattaaccaa accttcatca tcattttttg tggcaatagt
     3601 tttgctaata aatgactgga ttttgtattt ataccataca cagaaatatc cttatttttc
     3661 tttgcatcct tcaatgcttt tttggcaaca atgtctggag acactatgcc tgggaaattt
     3721 tttgtccctt tttcagctac aactatacct ctcttaaata gatttgtact catccaacct
     3781 ggacatacag caattgcatt tatcccttta ctcttaagtt caacatttaa tgctcttgta
     3841 taatttctta taaacgcttt agttgaacta taaatatttt gatatggcaa tggctggaat
     3901 gatgcttgtg aagaaatatt catgatatgg cttccctttt gcatatatgg aatacatata
     3961 agtcccaatg ctacaacagc actaatattt aaatcaatca tattaataga ttcatctata
     4021 cttaaatcat tgtatgaaca gaattttgca aaaccagcat tgtttataag gtattttata
     4081 catacatttt ctttacttaa aaatgtacca atttctttta cttcttctat ttttgataaa
     4141 tcttttgaaa aaatttttac cttacttcca aattcatcta ctaattgatt taatctcgcc
     4201 ttgtttcttg caactgccca tatttctgtt atttcttctt catttattaa taactttaga
     4261 aactctttac caaaaccact attagcaccc gttataattg caatcttctt ataactcata
     4321 attattctcc tttaccataa gtttatacgc tcaaactgta aacttatttt ttacaaataa
     4381 aacaccctct attcagatgt tatattcata ttatttgaga tattatattt atatcatttt
     4441 caataattgt tatatagcat aaaaaatagt gttaatatta tagaatcggt attgattata
     4501 tt
//
LOCUS       DAMJWS010000150         4466 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMJWS010000150 DAMJWS010000000
VERSION     DAMJWS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.155"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            210..1349
                     /locus_tag="PC399_003602"
     CDS             210..1349
                     /locus_tag="PC399_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2594405.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1375..1563
                     /locus_tag="PC399_003603"
     CDS             1375..1563
                     /locus_tag="PC399_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594406.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            2347..3903
                     /locus_tag="PC399_003604"
                     /pseudo
     CDS             2347..3903
                     /locus_tag="PC399_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
BASE COUNT         1951 a          468 c          741 g         1306 t
ORIGIN      
        1 ttataatatg taactaatgt attataaata atagttgtaa acttcctgtg acatatattt
       61 taaatgctta ttcttaaatt tttgcattat ttattaaaat ttattatgct ttaacgctaa
      121 attgagtttg ataattataa aacaaataat caaaaatagt gtaaaaatta atactttttg
      181 ttaatattaa tagataaaga taatctaagg tggtgattaa actgacaata gaagaataca
      241 aaaacaaata ctttactgga aataaaatcg tactttctat aaatagtttt gataataaag
      301 aactatacta tcatgaaact gaaaatatat taaatttaat aaatgaaata gataaattaa
      361 catcaagtaa gatatttaaa acaatagaag ataaaaatga tttggcacta gagtcttatt
      421 attctagtgt aatagaaggt gctttttcaa ctagaaagat tgcaaaatct ataattagag
      481 gaaagatgaa accaagtaat aaaagtgaat atatgattta caataatcat agagccttag
      541 aatatggatt agacaactta gataaattat atagtcacaa atttatatat gatttgcatc
      601 atatattggg cgaaaactgc ttggattcag aagaatatga gtatagaact gaaaaagtat
      661 atgtatgtga ttcaaaaggt gagattatac atacagggct tgaacctttg aagatatatg
      721 actttatgag taaattaata gattttatgg aaaatagcaa agtaagtaat ttaattaaaa
      781 gtgctataat tcatttttac tttgtatatg ttcatccttt tagtgatggt aatggaagaa
      841 catcaagagc cttatcatat ctatatttga ttgataaagg atatgatact ttcaaggatt
      901 tttcaatatc atatatgata tctaaaaaca gaactaagta ttataaggct atacttgatg
      961 tagaaaacaa aggaaataat ttaactgtat ttatagaatt tatgctcaag tctataatac
     1021 aatctattaa tgagatgcgt aatatgcatg atagaaaatc tttagaaagt atattaaaag
     1081 aagaattgtt tgaaaatgac ataactctat cagcaacaga agaacatata ttaaaatata
     1141 tttgcaataa agataactat tctatgacat tagaaaatta tataaaaaag aataaaagta
     1201 gatacttaaa ggctggaata aaggaaattg aattagttga ccagttgatg gaagtgttta
     1261 ataatcttga agagatagag attttgtcta aagaaaaaaa tatatataaa gttaatgata
     1321 agtacttaaa aatgcttgat atagactaat ttagataatc tggagaggga atatatgaat
     1381 aataagaaaa aggcattttt gatttctggg gctataaacg taatttcatt aattattgtt
     1441 ttttcattgt ttcattataa tattatagaa aatagtacac aaaatatgat aataactgta
     1501 atcttaattt ttatttttga aatgataaaa ataaaaatca tagagaaata ctataaacta
     1561 taaacaagaa atatcttttc taagagcata ttgtacaata tgctcttaat ttagttatat
     1621 agatttaata taaataatgt attttttgta gatgatttat gtaagattta tgaccaattt
     1681 gtgtatgatt tgtgttcttt aatcatgaca atattatatt gtaaaaaata aatttaataa
     1741 aaaatgactt ggaatttata ttctaggtct tttttatata aaaatttaat aagtttcaaa
     1801 tgttggtaac tttgacagta gaaaacgtct attgagataa cgaggattct aattgagggg
     1861 gagagatatt agcacttaaa gtagtatatg caacagatat aatctttaca aaagtaattt
     1921 gaattaaaat gtaaatataa agaataatta aaaaaatatc gcaattattt gtttttcata
     1981 tggataaaat tggttattta atatataatt acaagtataa ataataaaat ttatttgtaa
     2041 aaagagtagc actttaattc ataaaggcta ctttttttat acaaaaccct attttttata
     2101 acaattcatt aacataaaaa tcaaacttac cttaaaatat aaatttacaa atttttcaat
     2161 caataagaaa atttctttat tccttacata atgactgcaa tgtaacaaaa taattacaaa
     2221 ttaattatga aatcattata gttagaaacc tctttaaaat tagatattaa gtatccttta
     2281 gaattagaac gggaacaaag ttaaaaaatt aagtaatatc aaatggacaa gggggaagaa
     2341 aataaaatga aaataccaaa gaagattgct gcaatgctta cagttacaat gatagctgga
     2401 agttcaacag caggcatagc tagtgcacaa actgtggcaa caaatttaac agggcaagaa
     2461 agatatgaaa ctgctgttaa aataagtcaa gatggatgga aaaatgctga tgaggtagta
     2521 atcgtaaacg actcatctat agcagatgct ctatctgcaa caccatttgc aaaagcaaaa
     2581 aatgctccaa tacttttaac aagtaaagat aaattaaatg ataagacgaa agcagaaata
     2641 caaagattaa aagctaaaaa agtatactta ataggtggaa cttctgtatt aagtacaaat
     2701 atagaaaaag aaataaaaga tttaaaaata tcttttgaga gaatctctgg agctgagaga
     2761 tatcaaacat ccttagaatt agcaaagaaa ttagatgcta taagtgatgt taagaaaata
     2821 gctgtagtta atggtgaaaa aggacttgca gatgctgtaa gtgtaggagc accagctgca
     2881 caaaacaata tgccagtaat acttgctgat tctaaaaatg gaacagcagt tgcagacaaa
     2941 tttataaaag atgctggaat aactcaatct tatgtagtag gtggagagag ttcaatttct
     3001 gaagctgtta aaaataaact accaaatagt acaagactag gtggaacaga tagaaatgat
     3061 acaaatgcaa aagtaataaa agaattttat aaaaagacag atttaaaaaa tgcttatgta
     3121 actaaagatg gtatgaataa acaagaccaa cttatagatg cactagcagt aggtgtatta
     3181 ggtgctaaaa atcaatcacc agtagtttta gttggaaaga acttatcagc ttcacaaaaa
     3241 tctctagtta actctaagag ttttgataag ataactaaag ttggtgggaa tggtaatgag
     3301 acagcattta atgaaatgaa gtcattacaa gaagtaaaaa cagttgaagc taaaacaatt
     3361 tctgaattaa aatcagctat agataaagct actgctaatg atgttataaa ctttaagcca
     3421 actagtgagg ttaaagaagc atttacaata caaactgata aagcagtaac agtaaactta
     3481 aatggtacat atacaaagac tgtaactatc aacatgccta atggagatgt aaataactat
     3541 gcaaaagtgg atgatgtagt tatagatgat gtaaaagatg gaacttttgt taactatgga
     3601 aaaataacta acttaaaggt taatgataaa aatggagcca agatagaaaa taattctaaa
     3661 ggtgagattg gtagtttaac agtagcatct ggagcaagtc aagtaaaagt gactaatggt
     3721 ggtaagataa ctacagttac aaataattct aaagggacta ctatagataa taaaggtaca
     3781 ataagtagtg ttaagggaga taattctcca acaataagtg gaaatagtcc aagttctaac
     3841 tcttcaggag gaagttcatc aagtggaggc tcttcacatg gaggaggaag ttcttcatca
     3901 aaaccaagcg ataagtcaaa aacaaagaca aaagcagaag aaaaaatagc agaggtaaca
     3961 aaactagtag gaaaagtaac agcagataat ttagaagcat caaaagtaac attaaaagca
     4021 gcaactgatg caatagaaga agcaaggaaa gcagggtgga cagaatcaga agttcaaagc
     4081 tttaaaggat atggagatat agcaaaagta caaagtgaga taactgaatt agaatcacag
     4141 gctaaagaag cagcaaagac aaaagcagaa gaaaaaatag cagaagtaac aaaactagta
     4201 ggaaaagtaa cagcagataa tttagaagca tcaaaagtaa cattaaaagc agcaactgat
     4261 gcaatagaag aagcaaaggc agcaggatgg acagaatcag aagttcaaag ttttgcagga
     4321 tatgaagata tagcaacagt acaaggtgag atagctgcat tagaatcatc acaggctaaa
     4381 gaagaagcaa agacaaaagc agaagaaaaa atagcagaag taacaaaact agtaggaaaa
     4441 gtaacagcag aaaatttaga agcatc
//
LOCUS       DAMJWS010000151         4441 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMJWS010000151 DAMJWS010000000
VERSION     DAMJWS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.156"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(892..1302)
                     /locus_tag="PC399_003605"
     CDS             complement(892..1302)
                     /locus_tag="PC399_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594407.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1518..2900)
                     /locus_tag="PC399_003606"
     CDS             complement(1518..2900)
                     /locus_tag="PC399_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2594408.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNGKDVLYTDSGEVSIEQSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2911..3270)
                     /locus_tag="PC399_003607"
     CDS             complement(2911..3270)
                     /locus_tag="PC399_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2594409.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3475..4254)
                     /locus_tag="PC399_003608"
     CDS             complement(3475..4254)
                     /locus_tag="PC399_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF2594410.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTVISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1545 a          749 c          399 g         1748 t
ORIGIN      
        1 tatctatttt ttcttactat tcttatccac atcatccata tatttatttt acgattactc
       61 tttcactcta accctttaac tatctatttt ttctgatatt cttatccacc atcacccata
      121 tatttatttt acaattactc ttttacccta accctttaac tatctatttt tttctggtat
      181 tcttatctac tattatctgt atatttattt tatgattact cttttaacct accccctata
      241 ctttatagta atatgttttt aacctttttc attattccca atatgcttta taactacaaa
      301 tactctcccc ccttaaaaat aataataaaa ttaatcaata aatttataat catattatta
      361 ctctatgtat taagcaatta ctatgccaat aaactttgat ttttttaaac aaatcttaaa
      421 aatcctaaaa tgctaatttt ctttaacttt taatgaatct aatagataac atttaatttt
      481 cgttatattt ttcagaatat ttctattatt atttttaaga aaaaaaaata atgttaacat
      541 ttcttaacat acattaaaaa atgttaacat tataaattgg gtttaatact tttactttga
      601 ctcgatttac ttttattcta cttcttactt tatttttaac ttagacttta tttttttatt
      661 acttatttga gtcttattta ttttattatc actccatatg ttcaattaca tttgtctaaa
      721 ttctttgata cttttattac ataattttaa caagcttaag tgttatttgg gtagtgcagt
      781 tgataattat ttaaataatc atcaactgca tgtttatatc attacagtag cttaatgtat
      841 ttaatcatac ttacttagga ttttgaaata tcttagagat tatatttaac attagtcaat
      901 tgctgcataa ttgtaagcaa ttgaaatttg agacccatct tcattttcta tagtgatagg
      961 cttcataatt tttaatcctt cagtttttat cttttctaat tcttgttcca ttttcttaat
     1021 tgtttcctca aggtcttttt gacttacatc tttgactgca ttagggtctt tagcatagtc
     1081 ttttaattct tgaatatgtt tagcatactc ttcataagta taatgctcaa caccatactt
     1141 ttccaattca gtagggtcag ttattcctga ttttattaga gattcaagtt taggtaaagc
     1201 aatattagat gaatcatttc catttgcaaa tactgctcca gttcctccta gtaccattgc
     1261 agataccatc attgcactta taagggattt ttttattttc ataaccaatc ttcctttctt
     1321 agagatttat ttgggtagtg tagtttgaaa tgactattgc aatagttatc aaccacatcc
     1381 ttagactatc acagtagtca tgtttatgtc aagccttttt aaaaaacttt tcaataaaaa
     1441 atactatctc aaaacaaaat taattatttt gagatagtat tttaattcta aatcttttat
     1501 attactaaga tttatggtta ctctatccat gttctatatt gttctttatt aactgctaac
     1561 aaattccctt ttcttgatat gacaaagcga ttatcattat agccactttg ctcaattgaa
     1621 acttctcctg aatcagtata aagtacatcc ttaccattac tagatttatt tacatacgtt
     1681 tcttcgctat tctcagaatt agttactttg ctcatagatg attcttcatg ctctctaaat
     1741 tcaattacag atacttttgc cgtactaaaa tgagtctctc catcactttt agctttacta
     1801 acaagaatat cagattctac tttgggagaa tataaatcag ttgatttact acttattgaa
     1861 aatccaattt tatcactttg ttcatttttt gttaaaccaa aagattcaac ctcattaatc
     1921 tctttatttt ctgttgcaaa aacaagacct gtcccacaaa ctaatacagt tgaaactaaa
     1981 aaagtaacat aagttgattt tttaaatttc ataatattaa ctattctctt ttctattttt
     2041 gtctttccaa atccactaca tattgtcatt gaaaatggat tattctcctc tgctagactt
     2101 attagtgtgg ttgcataatc agccctatat tcttcaccaa gtatactcat aaccttttca
     2161 tcacaactaa attctatgtc tttatttagt aaaaaaaaca tcacccaaac taagggatta
     2221 aaccaatgga caacaagagc tacaagcata aataacttta acagattatc aaagctatta
     2281 atatgaacca tttcatgagt taaaacaaag tctaaagttt gattattagc ataattcatt
     2341 tgctttggca aaataattcg tggttttaaa ataccagatg taagtggtgt cctagtaaat
     2401 tcagatatgc aaacaataaa tcttctaaaa cggctattct tacacatcca ttttttaatg
     2461 aactcatgat tttttatatg aattgaagtt ttaaaaagat taattatttt aaaatatcta
     2521 aagcataaaa gtatgctaaa cataatagta actataaaag acaaaatgcc caacatttcc
     2581 aacatcataa tattttcaat attagacatt atttcaaatt cgtcagaatt gtttttacct
     2641 gtagagtcac tagctttctt aagagtataa ttttctgttg atgtattacc caattccaca
     2701 tcaaaagtcg attcaattat actataaata ctaaaaccag aatttaaact tactggtatt
     2761 agaagtttaa ataaaacaat caaccacaat aatacaaata cctttttagg taatttttta
     2821 aataaacatg ctcttataaa aataactatt aaaagtaata ctccagaagt aaaactcatt
     2881 tctaacaatt taatgaacat aaatcttacc tcactttaat ttttcaacaa tttgtttaag
     2941 ttttgtaatc tcatcacttg aaagattttc tttattaata aaagctgaaa gaaatttttc
     3001 tgaagaccct ccaaacattt tattaattaa agtttctgtt tcctgttttt gtacttcctc
     3061 tttgcttatt aaagctttac aaacaaaatt cggctcaatt ctctctattg caccttttga
     3121 cactaatttt tttataacgg tatacgtagt gtttctattc catcctgttt cttcttttaa
     3181 aatttttact aactctccag ccttgctttc tcctctctcc catagaactt ccattacttt
     3241 aagttctgaa tcaaataatt ttatctccat ataaaatcgc tcctcaaata tagttatcac
     3301 aatagtcatt actataataa aaaactatca tagtagtcta tactatgtca atcatttatt
     3361 ttttaataat aaaaactgtc tttttaaaaa attcttataa aagacagttt tatcaatgtt
     3421 atactttaat ctttaattaa agccatattt tatttaaata tataactata tatcttagta
     3481 catagtataa gatttttctt tatctaatag tatatcctct acatctgggt tttcttttac
     3541 taattgtcta taaaaattac aattagtaac acctacatat ggaattagcc atagtagacc
     3601 tattccaaga gtagctattg aaagtatata ccatcctata aaactaaagt ttagtacaaa
     3661 taatttaagt acatttcctt tcatcatatc taaactagca cccattgatt taaatacacc
     3721 catattatcc ttgtcttcac aaattaagaa agtagcttga gaaacatata atagcaatat
     3781 ataaaccggt aacataagaa caagtattat aagaactaga gttattccag tagaaataac
     3841 agtccctatt ccaacattat cacttgataa atattcaatt gctccactag taagtaaaac
     3901 tccaaatgat aaaaacgctc ctataattgt gattataaaa gaataaatat aaacaataat
     3961 tgattcaaat aaataaaaac caaaacttct taaataaatt ctcccactta caaagatatc
     4021 tcctatcttg actttttcat ctttagtaat tttcaagaac atacatgtaa atcctgctga
     4081 aacgcaaaaa gctattacag ccactacaat agtagtcaat ggttcaaaag gtaatcctac
     4141 aattgatatt aatactatca atgcaatata tactgccata gaaattgcac atatccccca
     4201 gtttccagat aactgagctt tagatactcg ttttaattct tgccttgata ccataaaaaa
     4261 cacccctttc attttataaa aaaactttta aaaatataaa taatctttta acgtataata
     4321 ttaatatatc atattttcga ctatataaga tacaattatt caccatgttt tatcaagata
     4381 taccataatt gtattactag ttttatatag tcaacattac caaatacatc aaataaattt
     4441 g
//
LOCUS       DAMJWS010000152         4385 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMJWS010000152 DAMJWS010000000
VERSION     DAMJWS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.157"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            167..1642
                     /locus_tag="PC399_003609"
     CDS             167..1642
                     /locus_tag="PC399_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2594411.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1729..2142
                     /gene="speD"
                     /locus_tag="PC399_003610"
     CDS             1729..2142
                     /gene="speD"
                     /locus_tag="PC399_003610"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF2594412.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2172..3023
                     /gene="speE"
                     /locus_tag="PC399_003611"
     CDS             2172..3023
                     /gene="speE"
                     /locus_tag="PC399_003611"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF2594413.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3013..3891
                     /gene="speB"
                     /locus_tag="PC399_003612"
     CDS             3013..3891
                     /gene="speB"
                     /locus_tag="PC399_003612"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF2594414.1"
                     /translation="MKNNFYHMNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4057..4257)
                     /locus_tag="PC399_003613"
     CDS             complement(4057..4257)
                     /locus_tag="PC399_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2594415.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1723 a          494 c          756 g         1412 t
ORIGIN      
        1 gctaatgtat atgtttttga gtaacttcaa aaaaacactt gtaaaaatgc tgggatgtta
       61 atataataaa tatcatgagt ttaataaaag taaaaataaa aaatagaaat aataaataat
      121 aaatattaaa aatatttgaa tatagagagg agatgaattt gaaatcatgg atagcgagtt
      181 tgcaaatgca gttgaattaa gctatgagtg tgctccatta ttagagtcgt taaaggaata
      241 ctcagagaaa gatatagctt gttttgatgt gcctggacat gtaaaaaata gaggtgtagc
      301 tatattaaat aaatatctag gtgaaagtct catgaaaatg gatataaatt catcaccaac
      361 tatggataat gtatctgcgc caaatgggat tataaaaaat gcacaagatt tgttagcaca
      421 agcatatatg gctgatgagg cattctttat aacgaatggc acaactcaag caatacatgc
      481 aatgattttg agtgttataa aaccaggtga aaaagtatta cttccaagaa acatacataa
      541 atcagtaata aatgcgttaa tactttgtgg aggaatacca atatttatac aaccagaatt
      601 tgatgaaaaa ctaggtataa gtttaaatat tactcttgaa aaagttagaa cagagataga
      661 aaaagattgt aatataaaag cattattttt ccttaatcca acttactatg gagtttgtgc
      721 agaccttgag agtattatag aactatgcca taaaaataat gttttagtgt tagttgatga
      781 agctcatggt gcacattttc catttcattt agatttacct ccttctgcaa taagtttggg
      841 agctgacatg gtagctgtaa gtatacataa aactggagga gcattgacac aatcttcagc
      901 actcttattg aatagagata atgtaagttt tgaaaaggtt cttcaatcaa tcaatatgct
      961 acaatcaaca tcagcatctt atttgcttat ggcaagtata gatggtgcta gagttaatct
     1021 agttgagaat ggtgaaaagc aattgtcaaa agcattaaat ctatctaggt atgcaaaatc
     1081 taaattgaac aaaattgatg gaataaaagt tttatctaca gaaatattga aacaaaaagg
     1141 tgttaaattt atagatgaaa caaaattgtg tataaatgta aaagaattaa atttgactgg
     1201 atttgaagta tacgatttat tatataaaaa tttttcaatt caagtagaat tgggagattc
     1261 atataatata ctggcacttg tgtccattgg aacaaataaa tcagatatag atagattagt
     1321 aaaggcactt tctataattg caaagattta tagaaaagaa tcaacattaa atgaatttaa
     1381 tatggttcaa ataaatccaa ttattaaact taatccaaga gaagcttttt atgcaccaaa
     1441 ggaaagtgta gaaatcaatt catgtataga tagaatttgt ggagaatcaa taatggcata
     1501 tccaccaggt ataccgatta ttgctccagg agaattgata acagaagaaa ttatggagta
     1561 tattattttc ttaaaaaata gcaatgcata tcttacagat gtacaagata aaaacttaga
     1621 tagaatacta gtaataaaat aaattacttt attttgcaag ggcaagtgtt tgttaagtag
     1681 agtttatcaa atagaactta ttaaaagtat aaaggagttg gggttgaaat gaaatttgaa
     1741 acactaggaa gacatatact agtagaatac tataattgtg atgaaaacgt acttaaagac
     1801 ccacgtctta tagaaaattt tatgaatgat tcagcattaa atgcaaaagc gacaattgtg
     1861 gattctgtat tccaccattt taatccttgg ggggtttcag gagctgttat aattgctgaa
     1921 tctcatttga caatacatac atggcctgaa tatggttatg cagcagcaga tttttttact
     1981 tgtggtgata tagacccatg gaaaagtttt gaattattag agcaactttt aaagtctgaa
     2041 agaagcgagt ctacagaaat tccaagaggc ttaactacaa aaattaaaaa atattcaaaa
     2101 aaagatttag gtaagattac tcataaacca gaagcagtat aaaacataca agagaaagtg
     2161 aggattatta tatggaactt tggtatacag aagaatggac agaaaatgta agattttcta
     2221 taaaagtaaa taaacatcta tttgaaggaa agtctcaatt tcaaaggata gatgtgtttg
     2281 atagtgatga atttggaaaa tttctaacaa tagatggatt aatgatggta acatataaag
     2341 atgaatttat ataccatgag atgataactc atgttcctat ggctacaaat ttaaacataa
     2401 agaaagtatt agtcataggt ggaggtgatg gaggtacagt aagagaatta agccgttatc
     2461 ctcaaataga aaagattgac atggttgaaa tagataaaat ggttgtagat gtatctaaag
     2521 agtatatgga tatatgctca tgtaaattag atgataaaag agtgagttta tattttgaag
     2581 atggtgttaa ttttgttaag tgtgcacatg ataaatcata tgacctgata atagtagatt
     2641 caactgaccc aataggacca ggtgaagggc ttttctcaac tgatttttat aaggattgtt
     2701 atagaatact gactgatgat ggaattttag taaatcaaag tgaaagtccg tattttgact
     2761 ttaatgctaa agagatgaaa agagctaata aaaagttaaa acagatattt ccaatatcag
     2821 aagtttatca agcacatata ccaacatatc catctggtca ctggttgttt ggatttgctt
     2881 caaaaaaact taatccagta aaaaatcaag atagaaatgg atgggagaaa ttaagtttaa
     2941 aaactaaata ttataatagt gatatacatt taggttcgtt tatgttacct caatacgtga
     3001 aggagatgct ggatgaagaa taatttctat catatgaaca cttttatgag tatgaacaaa
     3061 aattatgaag aatctaatct tatagtattt ggtgttggat ttgatggaac tacttctaac
     3121 agacctgggg ctagatttgc aagtagctct atgagaaaag aattttacgg tcttgagaca
     3181 tatagtcctt ttttagattt ggatttagag gattataata tatgtgatta tggagattta
     3241 gaaattagtg ttggaagtac agaacaagtc ttaaaagaaa tctatcaaga gacatataag
     3301 attgttagag attcaaaggt accctttatg attggaggag agcatttagt tacattacca
     3361 gcctttaaag cagtacatga aaagtataat gatatatatg taattcattt tgatgcccat
     3421 actgatttga gggaagaata taataatagt aaaaattctc atgcaacagt aattaaaaga
     3481 atatgggata ttgtaggtga taataaaata tttcaatttg gtataaggtc tgggacaaaa
     3541 gaagaattta aatttgctac agaagaaaaa cacacataca tggaaatagg aggaatagat
     3601 acgtttgaaa atatagttaa tatgctaaat ggaaagaata tttatctaac tatagattta
     3661 gatgtattgg atgcatctgt gtttccagga acaggtacac cagaacctgg tggtgtaaat
     3721 tatagagagt ttcaagagat ttttaagatt ataaaaaact ctaatataaa tatagttggt
     3781 tgtgacattg tagagttaag ccctgattac gatacaacag gtgtatcgac agttatagct
     3841 tgtaaaatct taagagagtt atgcttaata atatctgata aaattaaata gagtatttta
     3901 tataaatagt atatattata atataagaag ttattttaag tttgacgctt ttctaaagta
     3961 acttcttatt tttgtaatta aaaataaata taataaacaa caaaaaagag ccctcgatag
     4021 ctccttttct aaaatatata tatttaaatt aaataattat attctattta cgttttcagc
     4081 ttgaggtcct ctattacctt gagttatatc aaagctaacc ttttcgcctt cttctaaagt
     4141 tttgtatcca tcattttgta tagctgagaa atgtacgaaa acatcatctt ctccctctac
     4201 agatatgaat ccaaatcctt tttcattgtt aaaccatttt actattccgt ttttcattat
     4261 aagaaaacct ccgcattatt aaaaaataca aactaaaaaa ataagtctgt tctattaata
     4321 taacataaat tttaaataaa agcaaataaa atttttaaaa aaattaatta cttttagaat
     4381 ttaat
//
LOCUS       DAMJWS010000153         4314 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMJWS010000153 DAMJWS010000000
VERSION     DAMJWS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.158"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            231..1310
                     /locus_tag="PC399_003614"
     CDS             231..1310
                     /locus_tag="PC399_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2594416.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="PC399_003615"
     CDS             1634..2002
                     /locus_tag="PC399_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2594417.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDNDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="PC399_003616"
     CDS             2119..2922
                     /locus_tag="PC399_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2594418.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="PC399_003617"
     CDS             3307..4197
                     /locus_tag="PC399_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2594419.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVFKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1650 a          472 c          808 g         1384 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagaacgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tgctacctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tctactaact
      901 ggattgtaaa gcatggttgt agaactctac taaaaaaagg aaatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagct atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttagcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggataa tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtttttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaacaa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaattaca tactcattag
     2941 gtaaattatg tagtatatgt gtttatagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga agtattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggacagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagagat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggggta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaggcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgaggcagtg aaagaactta atgattctgg aattttttgt
     3841 ggtatattat tgactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgtatgta tggtgtaaca
     3961 atgcgtagtg gtcagcgaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtatttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tgttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gatattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtatta atattttagg taattgctag agct
//
LOCUS       DAMJWS010000154         4154 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMJWS010000154 DAMJWS010000000
VERSION     DAMJWS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.159"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(271..1131)
                     /locus_tag="PC399_003618"
     CDS             complement(271..1131)
                     /locus_tag="PC399_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2594420.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1280..2170
                     /locus_tag="PC399_003619"
     CDS             1280..2170
                     /locus_tag="PC399_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2594421.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            2388..4142
                     /locus_tag="PC399_003620"
     CDS             2388..4142
                     /locus_tag="PC399_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF2594422.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1703 a          437 c          644 g         1370 t
ORIGIN      
        1 tataatattt aaatttgtat ttgattgtta taaagtaaag taagtaataa agttagactc
       61 aataattcta aaatttctaa gcatactatc taaatgagaa cggatttgga aatacttgta
      121 taaaacaata attgatacat ttaattttta tcttaaaaac aacaattata atatataaat
      181 agcaagtata atatataaat agcaagtata atataacata taaaaagttg aatttgtata
      241 tgttaattat acttgctatt tatttgttag ttattctatt tttttaaatc cattacctaa
      301 agcttcatgt atatctgtaa ttataataaa agcattaggg tcgacatttt ttactaattt
      361 tttcaattcg acaacttctt tcttttcaac tactactaaa agtacatttt tattttctcc
      421 agtatatgca ccttctcctt ttagaatagt tgcacctcta tctaaatctt ccattatttt
      481 tttacttata tcttttgaaa ggtcagatat aatagtgaat gatttggaat aattaaatcc
      541 ttcaataagc aagtcactaa cttttactaa aatgtataaa gccacagttg aataaagtgc
      601 aatttcaacc tgtctattta ctatacctga taaaacaact acaacgcagt ctacaatacc
      661 cattaaaaca ggtatactaa atgtaggaaa atatttattt agtaggagtg ccattaaatc
      721 agttccacct gtagagccat ttattctaaa aataagtcct tgaccaactc ccattaaaat
      781 agaaccagat ataattgcaa gatacatatc atttgttata tctaatgtag ccatatttgc
      841 agtaagctta agtgctatgg taagaaaaat tattcctaaa accgttttaa aacaatcttt
      901 ttttgaaagt attttgtaag caaatatgaa taatggaaca tttaaaagta aattgacaat
      961 ccaaagttct acaccgaata atgaatttaa aacaactcca agtcctgtta taccaccagc
     1021 agcgattgca tgtggattaa aaaataagtt aagagaaatg gccattaata tacagcctac
     1081 aattaatatt aaaatttctg aaaaagttat tctatctttt ttcaataaca tcactcctta
     1141 tatatagatt ataatatata ttatatagtt tttccaattt ttaataaaag aaataaaaat
     1201 atattataaa aacaatttga ttggaatttt cagaaaatta atgctataat taaaagtaaa
     1261 tttaaaggga ggttataaaa tggctaaatt aactctagaa catataaaag gaaatacata
     1321 ttatattcca ctccctacaa tagtaggtgt atacgtagat ggtaaggatg caattttaat
     1381 tgacagtgga aataataagg atacagctag acaagtttta agattattag aagaacataa
     1441 tttaaatcct aaattaataa taaacactca ttcaaatgca gaccatatag gaggaaatgc
     1501 atatttgaag aatcaaacaa aatgcaagat tgctacgact aaaattgaag gatattttac
     1561 agaaaatcca atattagaat cagcattttt atatggtggc tatccaagta aagcactgaa
     1621 gaataaattt ttattagcaa aggaaagtga agttgattat attatacctt caaatggcaa
     1681 aattgtagat acagaacttg aagcgatttc tcttccaggt cattattttg aaatgattgg
     1741 tgttagaact ccagataatg ttttgtttgt tgcagatagt cttacaccag agaatataat
     1801 tactaaatat cattttttct tcctactgga tatagattca cagtttaaga cactagaaaa
     1861 attgagaatg ttagaggctg atttctttgt tccaagtcac tctgtcaaaa ctacagatat
     1921 aaaaagttta attaatatta ataagaagaa gatggaagaa ataatagata atattaaaaa
     1981 ggtttgttat gagccagtta tgattgatga agttattgaa aaaatgtgtg atttgtacaa
     2041 tgtcaagctt gatgctaatc aatatgtgtt ggttggaagt acaatacgtt cttatataac
     2101 ttatttatat gagaataata tggttgaata tatatttgat ggtggtaaga tgatgataaa
     2161 agctgcgtaa atagtaataa ttttcaaatt atatgatgaa attgttttag tatgactgtt
     2221 atttgaaaag taatataaaa aaatttatat aaataagcta agatgattga tataaagtta
     2281 ctgataattt taaggtaata tatacgttac tataatatta tgttattatt tgtaaataag
     2341 gggataattt ataagcaaat taaagacctt aatttacagg aggaatagtg tatattatga
     2401 ataaaaaatt caaaaaattc agtaaaaaac taagtatatt aattttagtt ttgactatgt
     2461 ttctttttaa taatttaact gcaaatgcac agattaaaga agattcaata ataggaattg
     2521 atagatatga aacagcagga cttatagcgg atagacaaac ttatgatact gtaatattag
     2581 ttaatggaga taaaaattta tctgatgggc tttcatcaag tggattagct ggagctatta
     2641 acgcaccaat acttttgact aaaaaaagtg aaataccaaa agctacttta aatagactag
     2701 ataataagag tttaaatgtt gtaaaaaaag tatatataat tggtggttat aatactattg
     2761 aaaattcagt agaaaaagat ataaaaagaa aaggtattca agtggaaaga ataaatggta
     2821 acaatagaat tgaaactagt tataatgtag ctaaaaaaat aaatgaagta agtagtgtaa
     2881 aagaggtaat gcttacaaat ggatttgtag gagaggctga tgctatgagt atagcacctg
     2941 tagttgcaaa aaataaagga gctataatac ttacagatgg gaaatcagtt ccatttaaga
     3001 ctgaaggttt aaatgtttac gctataggtg gcaaatctgc gataaatgag gatttagtaa
     3061 atgaaactaa agcaattaga atcggaggaa atgatagatt tgagaccaat aaaaaggtaa
     3121 tggaaaagtt ttataatgga gctacagatt tttatataac aaatggatat caattagtag
     3181 atgcactaac attatctcct ttagccaaag aaaaaccaat cgttttagtt gctgatggaa
     3241 gtaataaagg tattttaaaa ggagctaaaa gtataacaaa agttggaggt attgatgcca
     3301 atatatataa acagtgttta gatatgatta aatataataa tatggatatg actccaaata
     3361 ttttaaaaca cttaacttca gttgaaggaa atgaagtttc tgaaatactt atagaatcag
     3421 ataatttagg tgtggttgtt gaaaagacga ataccaataa atttgagttt gattacgtaa
     3481 gtgtaactaa tgaaaaaaat tgtacttttt ctgtatataa agaaaattct tctaataata
     3541 taaaaaatgg aaagctagtt gtaagtgcta agaaaaaaat agataagaaa gatagtgatt
     3601 tgcaagatat gaatggcgac aatatgataa atgcaaataa agataggatg gtaaatgtga
     3661 taaaaattgg agttcccgat aaaatgtatt ctaattttaa agtagatgtt gaaagtggtt
     3721 cagtagaact ttataatatt aaaggaggag ctactgttga tgtcaatgac ggaattgcta
     3781 acattgttga taacaatgta acaaatcctt tcaatataaa tactaatagt gggatttcag
     3841 gtgtaacagc tgaaactata tcatctgaaa taaaatttag agcaaacaat ggaattgtaa
     3901 atgtaacagc tacaaatata aatggcaata taagtttatt tggaagcgat aataaagata
     3961 cttttgatgg aatatttaaa ttaaatctga aaaaagaacc atcaaattta cacttgaagt
     4021 taattggaaa tgggataaat aaattgccag aaggttggtc taaagattat attttaggaa
     4081 atggacaacc aattattgaa gttaaaaata atggcataaa taatatatca attggagaat
     4141 aaaaacatat gaaa
//
LOCUS       DAMJWS010000155         4136 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMJWS010000155 DAMJWS010000000
VERSION     DAMJWS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.160"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            65..1372
                     /locus_tag="PC399_003621"
     CDS             65..1372
                     /locus_tag="PC399_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594423.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1458..1720
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1846..2649
                     /locus_tag="PC399_003622"
     CDS             1846..2649
                     /locus_tag="PC399_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2594424.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2687..3298
                     /locus_tag="PC399_003623"
     CDS             2687..3298
                     /locus_tag="PC399_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2594425.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3330..4001
                     /locus_tag="PC399_003624"
     CDS             3330..4001
                     /locus_tag="PC399_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2594426.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1639 a          395 c          764 g         1338 t
ORIGIN      
        1 aaattatata aattaaatta tataatatat tgagttttac ataaaagcaa aggtgggaat
       61 aaaaatgaaa gaaattgctg atgctataac atcagagtat ttaagctatg gtatcaatga
      121 agatattagt atctatgaag gtagattttg catatattta gataaaaaat atagatgtaa
      181 tgggaaaatt tattataaaa tgacacctcc aatatctata agttttaaag cagacatagg
      241 ttgtgttgaa gagatagaca atgaagatga caatttagct ttagattatg ataatgctat
      301 tcttgaagtt catggatata aaattatttc tataacaata aatactttaa gtgaatttag
      361 tgtagaaggt tatatcaatg atgactgcat aaaatctaag aactcctatg ttgaatatgt
      421 agattttaat ataataaact tagataaaat acctggaaaa ctcattaaat acaatgataa
      481 ggtatacgca ggtagaattg aatttgatat aaatgattat gttgtaacta tagacaagcg
      541 atatgattat agaaaagaat taaaatcaga attaaagtct aaatctggag caataataac
      601 tcatataggt aggattagaa gaaaagatgg tagaattttt agaacaaata atacaataaa
      661 tcttttagat aggatatcaa cagctttgag ttttatgtgt ggaagatatg tagggttttg
      721 tttggccaaa gggtatagaa gtggtaatga agtttataga atatggaatg aaaatcaaat
      781 atctcctttt agatatgtac caacatggtc agatacactt tcaaattatt ataatatgga
      841 aaaatatata agccttatgt gtaaaaagct agaagatttt tattatggtt ctgctataaa
      901 gagtgttgtt gattggtaca tagaatctct tggaagtgct accatggaaa acaatataat
      961 atctgtacaa attgcacttg aaactttgtc atatgtaatt ctagtggaac aaaataagat
     1021 acttacagat gaagtatttg actgtaattt ggcatctaaa aatataagac tcttacttga
     1081 tacatgtaag ataccatatg gtaaacatga attaaatata tttgataata taataaaaaa
     1141 taaatttgat gatggagtgg atttagttat ttatttaaga aatagcatag ttcatccaag
     1201 tagaaagact catagagctg tgttagaggt tgaagatatt tggaatataa tttcaatagg
     1261 aacgagatat attgaacttg ttttactctt tattttaggt tatagaggag aatattcaaa
     1321 cagattagta gaaagatgtt atggtgaagt ggaagttgta ccttggaact agatttttat
     1381 atttatttta aaatatatat tgacatatat tgccttatgc tataaaatca atatcataat
     1441 aataattcag aataacatta aatgctaaga aaagaaatag taaaagtagt taatcttaaa
     1501 cagagagttt ccgattggtg agaggagcaa tagattttac tttgaacaca tctttgagca
     1561 ggactctgaa agagtagaaa tactttatta ggagatttcc ggaatccttg cacccgttat
     1621 agtgctagag tataataatt ttattgtact taaagaggtt agtgtcgtga ggcattaatg
     1681 aacagaggtg gaaccacgag agttaactcc cgtcctctag cagaagctag aggcgggagt
     1741 tttttaatat aattttttaa gcgcgcagag aattatattg caatagatac actttttaaa
     1801 aaataatcaa aatattttaa aaattaaaag gcgagggaga atattatgaa aaatatatta
     1861 aaaaaagtag gaatatttac aattatgtta ggtttattag gtggagttgt aggttgttca
     1921 aaaccagata atgaaaaaga taaggatgct tcaaaggaat ctaaaaaaga agtagttgta
     1981 gggtttgata atacgtttgt accaatggga tttttagatg aaaagggaga tacagtagga
     2041 tttgacgtag acttagctaa agaaacattt aaaagacttg gaatggaagt taagtttcag
     2101 cctatagatt ggtcaatgaa agaaactgaa ttaaatgatt caaaaactgt agatgtatta
     2161 tggaatggat attctataac agacgaaaga aagaaaatag tatcatatac agaaccatat
     2221 ttacaaaata agcagataat agttactcta agtgactcaa aaataaattc aaaagctgat
     2281 ttaaaagata aagaagttgg aacacaacaa ggttcaacag cacttgatgc agtggaaaag
     2341 gataaagatt ttatgaatag cttaaaaggt ggagcaccag ttctttatga tacttatgat
     2401 aaagcactta gagatttaga gataggtaga actagtgcag ttgttggtga tgaagtttta
     2461 attagatact atatgggaca aaagggtgaa gacaaataca aggtattaaa agacgatttt
     2521 ggattagaag attatgttgt agcaacttct aaagaaaatc cagaactttg tgagaaaata
     2581 aatgaaacat taaaagaaat gaaaaaagat ggaacttttg acaagattta tgataagtgg
     2641 tttaaataga agataagtta gctgttaaga agggggcact aaaggcatgt tacaaggttt
     2701 agagatagtt atagcaatgt tttgtataac attgatagta tcaattccat tggggattgg
     2761 agttgcattt ttaagattat caaagaataa attagtaagt ggtattacac aatgttatat
     2821 attgataatg agaggtacac ctttactttt gcaaatgata gtcatatttt acggattacc
     2881 tcttttagga attgtatttg acagatttac agctggagta gtagctttct tcttaaatta
     2941 tgcagcatat tttgcagaaa tatttagagg tggaatacag tcaatcgata gaggacaata
     3001 tgaagcttca aaagtactag gatttgataa gtttactatg tataagagag taatctttcc
     3061 acaagttttt aaaagaatac tagcacctat ttcaaatgaa gtaatcacat tagtaaaaga
     3121 tacatcttta gtatatatat taggattaaa tgatatttta agaatatctc aaatagcaat
     3181 gaacagggaa gcaagtctgt taccattatt tgaggcagga gcgatatatc ttatatttgt
     3241 agctatactt actaagggat ttgaattact tgaaaaaaaa tattcttatt atagataaag
     3301 ttacaatatt ataggaggag gttttttata tgttgaaaat caaaaactta aataaatcat
     3361 ttaaaaaaaa tagagtactt aaagatattt cttttgagtt agaagaaggt cagattggag
     3421 ttttacttgg taagtctggt gcaggaaaga ccacaatatt aaggtgtata aatggtcttg
     3481 aagaatttga tagtggagag attataatag ataatgaggt aataaaaaat aagagagata
     3541 tggcaaaaat acgaggaaaa ataggtatgg tttttcaaaa tttcaactta tttcctcata
     3601 tgacagtcct tgaaaatata attgaatccc cagttaatgt ttttaaagtt cctagaaaag
     3661 aggctgaaga gagggctaga gaacttttaa gattagttga tttagaagat aaattaaatt
     3721 cttatccatt tgaattgtca ggaggacagc aacagagagt tgctatagct aggtcatgtg
     3781 cattgatgcc aaaagtcctt tgttttgatg aacctacttc tgcacttgat attgatacta
     3841 ttcaaagagt agtcaatata atgaacagac ttaaagacaa aggtatgaca atattaataa
     3901 ttacacatga tgttgtcttc tcaaataatg ttgcagataa aattataagt ataaaagatg
     3961 gtatagtaga aaatgttcaa ataaaagaaa aaattgttta gagttttata aatataaact
     4021 agggtagaat tttataatat aaataaaggg caagtaaaat caaatatact atgccctttt
     4081 gttatctcaa aatttaatct tatattttat gtttaagtta cattataatt tataat
//
LOCUS       DAMJWS010000156         4101 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMJWS010000156 DAMJWS010000000
VERSION     DAMJWS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.161"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..394
                     /locus_tag="PC399_003625"
     CDS             <1..394
                     /locus_tag="PC399_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2594427.1"
                     /translation="KKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNIKIN
                     LNIKGYKFLPYVKNLQASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFGNM
                     KSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            382..1320
                     /locus_tag="PC399_003626"
     CDS             382..1320
                     /locus_tag="PC399_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2594428.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1704..1928)
                     /gene="secG"
                     /locus_tag="PC399_003627"
     CDS             complement(1704..1928)
                     /gene="secG"
                     /locus_tag="PC399_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF2594429.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2521..3813)
                     /gene="eno"
                     /locus_tag="PC399_003628"
     CDS             complement(2521..3813)
                     /gene="eno"
                     /locus_tag="PC399_003628"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF2594430.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1418 a          657 c          458 g         1568 t
ORIGIN      
        1 aaaaaaaata gaacatgtgg tcattactaa taataatata gaaaattata tcaattttta
       61 taaaaaattt ggtttttcat ctaaagaaat gagcaaaaat tataaactta atgctagaaa
      121 tattaagata aatcttaaca taaaaggtta taaattttta ccttatgtta aaaatttaca
      181 agcaagtaga gataacttat gttttaaaat aaacacaaat attgatgagc caaaaaaata
      241 cttagaacaa aatgagattc aaattaagcg aggaattgta gaatgtacag gtgtctttgg
      301 taatatgaaa tctatttatt tgaaaaaaac taatggaaat ttaattgaat tatcaaatca
      361 tagaatcacg gagagtaaac cttggaaaat ttaatcctat caataaatgt tgttcttcct
      421 ttgtttctca caatgtcact tggatatgtt ctaaaaaaat taggtctatt tgatactcat
      481 actttaaata aaatgaataa tgtctgcttc aagtctttct tgccattatt attatttttt
      541 aatatataca atacagattt agatggttct cttaatttta ggcttatggc tgtagctgtt
      601 ataattattt tttcaatctt cttaatctta gtctttatta ttcctaaaat tgaaaaagat
      661 aataagacaa ggggtgcatt aatacaaggt gtatttcgta gcaatttcgt tatatttggt
      721 attcctgtga caatttcact atttggcgat aagagtattg gagtcacatc tttattaatt
      781 gcagttattg tacctatgtt taatgtatta tctgttgtaa ttcttgaaat ttttagaggt
      841 ggcaaagtaa atataaaaca tatgattaaa agcgtcttaa caaatccact cataatcgcc
      901 tcttttatag gattgacaat gctaatttta aatattaata tacctacatt tttacacaaa
      961 actattggtg atatttctaa aatagctact cctttatcac ttataattct aggtggctct
     1021 tttagttttt ctgctataaa ggggcatata aaccccatta tattgggcgt atgtggaaaa
     1081 cttatacttg tacctgctat atttttacca attagcattt ttcttggatt tagaaatgta
     1141 gaattagctt cacttatgat aatgttatct gctccaactg cagtttcttc atttacaatg
     1201 gcacaacaga tggattctga cagtgaatta gctggacaaa ttgtagtatt tacttctgga
     1261 ttttctataa ttacagtatt tttaataata tttattttaa agcagaataa tcttatttaa
     1321 tgatatttga taacacaaat tggtattatg agaaacatat ataaaatcga tttaattgcg
     1381 taaaaacata ttattatact atttttacta tataatataa ctttgtgtat attatctcat
     1441 taaacatggt gaaagtagtg ttgctatata ttttttagtt tgaaaaaaat atataaatac
     1501 aaacaatata taaatacaaa taataaacgc caacaaatag tcaatctata aaataaaaac
     1561 tggactaatc atataaaatc attagtccag cttttatttt acgtactttt tatgttgatg
     1621 tacttttatt aaaaactaaa tttaattttt aaatgtctag caaatgcact aattaaataa
     1681 tggccagtta ttcaacaata tcactacttc ttaactataa gtaatgccaa tgcatttata
     1741 aaaaataata ctgctgttat ctttgtaact ctagcaagaa atgcttgttt tccttttggt
     1801 ttaaagtaag cttggcttcc ttctccacca aattctgaag gtaaagcatt cttagaatct
     1861 tgaggcatta tgctaattac tagtaaaatc cctagtacta cttgcacacc cattaaaatg
     1921 ttgctcattc ttgcccctcc tatctgttta ttacttaaat ttcattactc tttagtgtat
     1981 cacaataata tagccatttc aagactatta tgctataaat aacatacaaa aaaatatatt
     2041 gttactataa tatattacta tattctcaat ataaaaatga ataaaactta gaagagaaaa
     2101 ctatttaaat taatgcataa attaacactt aaaataatct actctcattg tatatattat
     2161 aaatagccac caaaataaaa caatcatttt aaaatattga tataactttt aatttcaaca
     2221 taatttaaaa taaataatcc acatttagca ataatacttt aatgacaatt atgattaaat
     2281 caaaatatgt cttcactttt attttttaat aaaaacctcc tatgcaatta gataaaaatg
     2341 agatagaaac atttattcat attttttctg aatctatgaa taaaattaag taataaaata
     2401 taaaaaagga taagtgaatt gaaaaatcaa ttaacactta tctcttttat atcttataag
     2461 atgagcttaa atatcttata ttctctcttc attaatcttt tttagtaacc attttaataa
     2521 ctattttttt aagttataga aagattttaa tccacaatat ctagcttgct ctccaaccat
     2581 ttcttctatt cttaataatt ggttgtactt agcaactcta tcagttcttg atggagcacc
     2641 agtttttatt tgaccagcat ttactgcaac agctaaatct gctatagtag aatcttcagt
     2701 ttctcctgat ctgtgagata taacagcagt atatcctgct ctcttagcca tttctatagc
     2761 atctaaagtt tcagttattg tacctatttg attaacttta actaatatag agttagctac
     2821 tccattttca attccttttt ctaatctctc tgtgttagta acaaataagt cgtcaccaac
     2881 taattgtaat ttatttccta atttttcagt taataatttc catccatccc aatcttcttc
     2941 gtctaaacca tcttctatag ttattattgg gaagttgttt gaccaatctt catataaagc
     3001 aaccatttca gcagcagtta actcttttcc ttctcctgct aaaacgtatt ttttagtttc
     3061 tttgttgtac atttctgtag cagcaacgtc aagacctaac ataacgtctt cacctggctt
     3121 atatccagct tttgttatag cttcaactat taattctaaa gcttctctgt ttgaacctaa
     3181 gtttggagca aatccacctt cgtcacctac accacaagct aatccttttt ctcctaaaac
     3241 tttctttaac gaatggaata cttctgctcc cattcttaaa ccttctttga aacaacaagc
     3301 tcctactggt aatatcataa attcttgaac atcaacgttg ttatcagcat gctctccacc
     3361 gtttaatatg ttcatcattg gaacaggtaa ttgtttagca tttactccac ctaggtattg
     3421 gaataatggt aaacctattt catcagcagc tgctctagct actgccattg aaacacctaa
     3481 tatagcatta gctcctaatt tacctttgtt tggagttcca tctaattcta tcatgattgc
     3541 atctatagct ggttggtcaa atgcatccat tccttctatt tcaggtgcta ttatttcatt
     3601 tacgttagca acagctgttt ctacaccttt tcctaagtat cttcccttat ctccatctct
     3661 taattcaaca gcttcaaaag ctcctgtaga tgctcctgat ggaactattg ctcttcccat
     3721 tgctccatct tctaatacta cttcaacttc tacagttgga tttcctcttg agtctaatac
     3781 ttctctagca tatactaatt caataactga catttgtata tctcccttcg attttctatt
     3841 agttaaattt tcatttactg taaacatccg tctactattt taatctactt tctatattat
     3901 agtacataaa gttgcttttg aaaattaggt acaaatttat ttgatgggta aatttgtaaa
     3961 agttcctatt tttattttat tttttataat attttaattt atataatata aaaaaacttg
     4021 tctaaaatcc aaaaccaatc ttaaatacac aaatattata actagattta aaacattaaa
     4081 cataaaatgt acaactaatg a
//
LOCUS       DAMJWS010000157         4093 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMJWS010000157 DAMJWS010000000
VERSION     DAMJWS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.162"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            118..1521
                     /locus_tag="PC399_003629"
     CDS             118..1521
                     /locus_tag="PC399_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2594431.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1612..1852
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1895..2593
                     /locus_tag="PC399_003630"
     CDS             1895..2593
                     /locus_tag="PC399_003630"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF2594432.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2616..3617
                     /gene="trpS"
                     /locus_tag="PC399_003631"
     CDS             2616..3617
                     /gene="trpS"
                     /locus_tag="PC399_003631"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF2594433.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1540 a          457 c          732 g         1364 t
ORIGIN      
        1 aactgttttt gaatatgcaa atattttgaa aaatatcagt ttaataataa tgaatctatt
       61 ttattaattt tgaaattaaa tttaaaataa ttaatagaga ggggaaatta aattaggatg
      121 gaaaagaaaa aaataggcct atggagtttg gtatttatga atgtatcagc tttatatggt
      181 ataagatgga tagctaaatc tacagcatca agctttggtt taggattagg tgcaatacct
      241 atgtggttat tattctcatt tatatacttt gtaccaggag ctttaatttg tgctgaatta
      301 gcttctacat atcaaaaagg tgatggaggt ctttacgatt gggttaagga agcctttgga
      361 gaaaagtatg gatttttagt ttcttggtta aactggacag ctaaaatatt ttggtattct
      421 tcatttttaa ctttcttagc agtaaatgtt tcttatacaa taggaaatcc agcccttgct
      481 gataataaga tgtttgtact tatattatct ttaataatat tctgggcatt atctcttata
      541 gcaactaaag gaataagttt tacaaaaata tttactaata caggtgcatt aggttcaaca
      601 ataccaagtg ctatacttat aatattttca tttgtagcag tatttgtatt gaaaaaacat
      661 gatatagctt ctacatacac aatagcttca atgactccaa aacttaatat ggatgcattt
      721 gttgctatat cagctataat gtttggttta gcaggagcag agactgctgc taacttcata
      781 acagaaatag ataagcctga aaagaacttc cctaaggcaa tattaatatc agctggaata
      841 gtagcttcat tatatgtatt aggttcaatt gcaataacaa tgataatacc accagaccaa
      901 attactgctt ctaaaggtgt attagatgca ctttctgctg tttgtgcgtc tttaggaatt
      961 ggttcttggt tggttcaatt aatagctttt ggtatagctt tttcagtatt aggagcaata
     1021 gttctttaca tagcatcacc aataaaaatg ttatttggta gtgttaaaaa aggaatattt
     1081 ccagatagtt tagtagaggt aaatgaacac aagatacctt ctaaagcagt tatacttcaa
     1141 gcaataatag ttactataat actattggtg actacattaa tgccttctgt tgatgctata
     1201 tacaatgttc ttgtaactat gacagcatta actgcattat tcccatatgt attgttatat
     1261 gcttcttata ttaaactaag aaaagagaga ccagatgaaa ttagacctta tactatggca
     1321 aaatcaacaa gtacttgcct aggtttagca aaaatggttt tagttgtaac tgtagtgggt
     1381 atattattat ctgcagcacc agttatgcct acatttgctg aaaatgttat atatgagata
     1441 gagatgattg gtggaggtct tctagttata ttatctggtc tatggttatg gaatagatat
     1501 gaaaagaaaa cagctaaata aaatgtattt taaaaatttc ttttggatat tataatatat
     1561 agttgacaat ttatagtgta aggaatatta taaaataaag aaattaataa attaaatcct
     1621 gagataagaa atagtaaata aatatatttt tgcacagaga attaaggatg ctgagaactt
     1681 aataaaaagt atttattgaa tgggtctttg aggagtaaaa tgaaatgtaa tttaagatta
     1741 cagagtattt gatatcgtgt atgcacgtta tagcatatat taagatatat atattcaatt
     1801 ttgagtgtat ataaatttag gtggtaccac ggaaaatata tccgtcctat tgatttagga
     1861 cggatttttt aatatagaaa atttaggagg aaaaatgaac gtaataggaa ttataggtgc
     1921 aatggatgaa gaagttagta ttttagttga tttaatggat attagagaga ctataaaaaa
     1981 agctagctta gaattttata agggaatttt ggaaggtaag aatgtagtgt tagtaaagtg
     2041 tggcataggt aaggttaact ctgctctttg tgcccagata ctaataagcg aatttaaggt
     2101 tgatgctatt gttaatacag gtgttgcagg agctttaaac gaaaaacttg atgttaatga
     2161 tatagtaata tcaactgatg ctatacagta tgatgtagac actacagcat ttggagaccc
     2221 taagggagtt ataccaagaa tgaagacttc tgtatttaaa gcagatgaaa gacttataga
     2281 tgctgcttac aaatcttctg tggaagaagt aaagactcat aaagtattaa aaggaagagt
     2341 agttacagga gataaattta taaattctaa agaattaaaa gaagaattag taaatgattt
     2401 tggtggatat tgtggagaaa tggaaggtgg agctatagct catgtatgct atttaaataa
     2461 tactccattt gtaataataa gagcaatgtc tgataaggca gatggaagtg ctgatgttac
     2521 ttatgatgta tttgtacatg atgctgccaa taactctaaa gacatagttt taaatatgtt
     2581 aaaatcaata taaatatata aggaggtaca aaaaaatgag tgaaaaaaaa gttatattaa
     2641 gtggtgcaca gccatcaggc aaaatgactt taggaaatta tttaggagct ataaaaaact
     2701 ggactgaact tcaagacaat tatgattgtt attatagtat agttgatttg catgcaataa
     2761 cagtacctca agaccctaaa gttttaagag ccaatactat agaattactt gctcaatata
     2821 tagcttgtgg attagaccca gaaaaaaata ctatatttat acaatcacat gtcaaagagc
     2881 atgttgaact tatgtgggta ttgaatacta tgacttatat gggtgaatta agtagaatga
     2941 ctcaattcaa agataaatca caaaagagtg aagcaaatct aaatgcaggt ttattcacat
     3001 atccagttct tatggctgct gacatattgt tgtatcaaac tgacctagtt ccagttggag
     3061 atgaccaaaa acaacattta gaattagctc gtgatttagc aaataggttt aataatagat
     3121 tttcacctac ttttgtagtt ccagaaggat actacccaaa aggtggagca agagtgatga
     3181 gtttgcaaga gccaactaaa aaaatgagta aatccgattc aaatgaaaat gcatttatac
     3241 tattagcaga tgattcagat tctataaaga gaaagataaa aagaagtgtt actgattctt
     3301 taggtgttgt aaaatacaat gatgaacaac caggtattaa aaacctaatt gatatatatt
     3361 ctaatttaag taaaaagagt gtagaagaga ttgtaaacat gtatgaagga aagggttatg
     3421 gcatatttaa agaagatgtg gctgaagtag tatcagaagc tttaagacct ataagagaaa
     3481 aatatgttga ccttcttaat aataaagatt atttagaaaa aatatatagt atgggagcag
     3541 aaaaagccga aaaacaagct agaaaaactc ttagaaaagt ttataaaaaa gttggattta
     3601 tagagagaag atactaatta ttagctatct ttacttgata aataaatttt aaagttatta
     3661 aaaaggtata tgatatgaaa gagagagtct caaagtgaac ttttgttcat gaggcactct
     3721 tttttgtatg aaataaaata tattttaatc atttcaacaa tgaaaaagag gattatctct
     3781 attttgagac agcatctttt gataataaaa aaagatgagc ttttgctcat ctcaaagggg
     3841 ttagattatt cttactttgg ggaaagtaga ataaaggtaa aagttaactg atgtcaactt
     3901 tgtctttttt gcatatcact actgagcata aaaaaatcag aaaaccatag ccttctgaaa
     3961 caacttctaa catacattgt attttatacc caatatgtaa tttatgagtt aattttaact
     4021 cttataatga aaattgtcaa taatatatca aaaaaatatt ttttaaattt atataattaa
     4081 atgaatttat gtt
//
LOCUS       DAMJWS010000158         4085 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMJWS010000158 DAMJWS010000000
VERSION     DAMJWS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.163"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..369
                     /gene="tuf"
                     /locus_tag="PC399_003632"
     CDS             <1..369
                     /gene="tuf"
                     /locus_tag="PC399_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2594434.1"
                     /translation="DNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEE
                     GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLR
                     FSIREGGRTVASGVVATIIE"
     gene            619..768
                     /gene="rpmG"
                     /locus_tag="PC399_003633"
     CDS             619..768
                     /gene="rpmG"
                     /locus_tag="PC399_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF2594435.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            794..1015
                     /gene="secE"
                     /locus_tag="PC399_003634"
     CDS             794..1015
                     /gene="secE"
                     /locus_tag="PC399_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF2594436.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            1035..1577
                     /gene="nusG"
                     /locus_tag="PC399_003635"
     CDS             1035..1577
                     /gene="nusG"
                     /locus_tag="PC399_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF2594437.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1616..2041
                     /gene="rplK"
                     /locus_tag="PC399_003636"
     CDS             1616..2041
                     /gene="rplK"
                     /locus_tag="PC399_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF2594438.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            2112..2810
                     /gene="rplA"
                     /locus_tag="PC399_003637"
     CDS             2112..2810
                     /gene="rplA"
                     /locus_tag="PC399_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF2594439.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2862..2990
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3033..3539
                     /locus_tag="PC399_003638"
     CDS             3033..3539
                     /locus_tag="PC399_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF2594440.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3597..3962
                     /gene="rplL"
                     /locus_tag="PC399_003639"
     CDS             3597..3962
                     /gene="rplL"
                     /locus_tag="PC399_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2594441.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
BASE COUNT         1657 a          494 c          828 g         1106 t
ORIGIN      
        1 gataatatag gagcattatt aagaggagta caaagaaacg agatagaaag aggacaagta
       61 ctagcaaaga ctggatcagt aaaggcacac acaaagttta cagcagaagt atatgtactt
      121 aaaaaagaag agggtggaag acatacacca ttctttgatg gatatagacc acaattctat
      181 ttcagaacaa cagacgtaac aggagcttgt aagttaccag aaggaataga gatggtaatg
      241 cctggagata acgtaacaat ggaagtagac ttaataaact caatagttgt agaagaggga
      301 ttaagattct caataagaga aggtggaaga acagtagctt caggagttgt tgctacaata
      361 atagagtaat tctttactaa actgatggat taaaaggaat aacaaaaaga ggggttatgc
      421 tcctcttttt ttaacataga aatacaaaaa cattgacaca gcataggatt ttgtgataac
      481 ttatacaagg taatgattat aggaagttaa taatatcttg aatactgtag atttttaata
      541 aatacatatt ttaaagatat tatattcaca tatattaaca atatatatta ttgtattaca
      601 aagttggagg tgtatcagat gagagttaaa gtaactttag catgtacaga gtgtaagcaa
      661 agaaactaca acactactaa gaataagaaa aataatccag acagaataga attacaaaaa
      721 tattgtagat tctgtaaaaa gcatacaact cataaagaaa caaaatagtt ttttataagg
      781 atgtgagtat ggaatggctg cccaaaaaaa tgacggtgca aaaactaaaa aaaggtttag
      841 tttgtttgga tacttaaaag aaactaaaca agaattaaaa agagttacat ggccgacaaa
      901 aaaggaactg tttaaaaaca caagtatagt tttaactgta gttatttcat gcactatatt
      961 agtatggggt atagacacta tattatctgg tgcactagcg ttattactaa aatagacgaa
     1021 aagaggtaag ttaaatgtca gaattacaag aagcaagttg gtatgtagtt catacctatt
     1081 cgggacatga aaataaagtt aaagctacaa ttgagaaagc agttaaaaca agaggtatgg
     1141 aagactgcat aagacaagta gttgttccaa cagaagaagt agtggaaact actaaaactg
     1201 gaaaagaaaa aactagacaa cgtaaagttt atccaagtta tgttttggtt aaaatgataa
     1261 taactgatga atcttggtat gtagtaagaa atactaaggg agttacaggt ttcgttggac
     1321 ctggttcaaa accagtgcca ttaagtgaag acgaagtcaa agctatgggg atagacacaa
     1381 cagaccctaa agtagtaaat agcgatattg attttgaaat aggagataca gtaaaagtat
     1441 ctcaaggacc atttagtgga caaataggta acatagaaga aattgactta gaaaatagag
     1501 aagtcaaagt atgcataaat gcatttggta aaaggactct atttgtaata gagcttgaag
     1561 gtatagaaaa aatctaatac tatattatag aaaaatctga ggaggtgcgc tcaaaatggc
     1621 taaaaaagtt ataggtcaaa taaaattaca aatacctgca ggaaaggcta ctccagcgcc
     1681 accagttgga ccagcattag gacaacatgg tgttaatata atgggattca caaaagaatt
     1741 taatgctaaa actgcagatc aagctggaat gataatacca gttgttataa ctgtatatca
     1801 agatagatca ttcagtttta taacaaaaac tccaccagct gcagttttaa ttaaaaaagc
     1861 attaaactta aaatcaggtt ctggagaacc aaacaagaaa aaagttgcta aaatgacttc
     1921 agctcaagta agagaaatag ctgaattaaa gatgcctgac ttaaatgctg catcagtaga
     1981 agctgctatg agtatgatag ctggtactgc tagaagcatg ggtgttgtaa tcgaagacta
     2041 gttaaaagat acactttttt ttataaagtg ggaggctaga agccgaaatt aaccacgagg
     2101 aggaaaataa aatggctaaa aaaggtaaaa gatatgcagg tgcattacaa aaagtagata
     2161 gaactaaatt ttatgatgca tcagaagcat taacattagt ttcagatata gctggagcta
     2221 aatttgatga gacagtagaa gcacacatta aattaggtgt tgactcaaga catgctgacc
     2281 aacaagtaag aggtgcagtt gtattacctc atggaactgg taaaacaaaa agagttttag
     2341 tttttgctaa aggtgaaaaa gctaaagaag ctgaacaagc tggtgctgac tttgtaggag
     2401 ctgaagaatt agttcaaaaa atacaaggtg aaaactggtt tgactttgat atagtagttg
     2461 ctactccaga tatgatgggt gtagtaggta gattaggtag agtattagga cctaaaggtt
     2521 taatgccaaa ccctaaatca ggaacagtta catttgatgt agctaaagct atagatgaaa
     2581 taaaagctgg taaagttgaa tacagattag acaaaactaa cataatacac gttccagtag
     2641 gaaaagtatc atttggtgga gaaaaattaa ctgaaaactt tactgcatta atggatgcta
     2701 taataaaagc taaaccagct gctgctaaag gacaatattt aagaagtata acagtagctt
     2761 cttcaatggg acctggagtt aaaataaacc cagcaagaac agctgaataa taaaactgtt
     2821 gacacaggtt gacaaaaatg ttattatatc acttgtcaat gtaaaaaaga atagatttgc
     2881 cgtagatagt gggtgcgaaa gcttaatatc ccgccgaggt tttagataaa ttgagattac
     2941 taaagctttt gatgtctact tttggcaaca aaagcttttt ataatgttgc ggtaaatact
     3001 aagcctattt ggtgaaggag gtgcacatct atatgagaaa agctatagaa attaaatcag
     3061 aagttgtttc tgagatagtt gaaaagttac aaaaatcttc tgctgctgtt gttgtagatt
     3121 acaaaggatt aacagttgaa gaagtaactg agttaagaaa acaaatgaga gaagctggtg
     3181 tagattacaa agtatacaaa aatactttag ttagaagagc agctaaagaa gttggtatag
     3241 agcaatttaa tgatgaatta ttagttggaa ctaatgccat agcatttgga tatgatgatc
     3301 cagttgctcc agctagaata ttaaaaggat tcatggattc tcatccaaaa atgaaattaa
     3361 agatgggtat tgtggaaggt gcattctatg atgaatctaa gattgttgaa atggctaaca
     3421 taccatcaag agaagttctt atcgctaaat tacttggtag cttaaaagct ccagtatcaa
     3481 acttcgcata cttaatagat gcaatagcga aaaaggcaga aggtcaagaa gaagcttaat
     3541 tgcttaacta aaaaattata aatattaatt tgaaataaaa ttcggaggtg ctaaaaatga
     3601 caatagaaca aatattagaa gctatagaaa atatgaaagt tttagaatta aatgaattag
     3661 ttaaagctgc tgaagaaaaa ttcggtgtat cagcttcagc tccagtaatg gtagctggtg
     3721 cagctgctgg tggaccagct gctgaagaaa aaactgagtt tgatgtagta ttaactgacg
     3781 ttggttcttc aaaagttgga gttataaaag cagttagaga aataacagga ttaggattaa
     3841 aagaagctaa agaagtagtt gacaatgctc ctaagacagt taaagaagga gcttctaaag
     3901 aagaagctga tcaaataaaa gagaaattag aagctgctgg agctaaagta gaagttaagt
     3961 agtttcttta aaaagatacc cattgggtat ctttttttat ttataaaaaa ctataaagaa
     4021 agtatttgga attataaaga aagtattttt ataaaataaa aaaaatttat ataatagtac
     4081 taaat
//
LOCUS       DAMJWS010000159         4084 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMJWS010000159 DAMJWS010000000
VERSION     DAMJWS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.164"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(202..585)
                     /locus_tag="PC399_003640"
                     /pseudo
     CDS             complement(202..585)
                     /locus_tag="PC399_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      811..908
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            963..1139
                     /locus_tag="PC399_003641"
     CDS             963..1139
                     /locus_tag="PC399_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594442.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1365..1565
                     /locus_tag="PC399_003642"
     CDS             1365..1565
                     /locus_tag="PC399_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2594443.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1729..1941)
                     /locus_tag="PC399_003643"
     CDS             complement(1729..1941)
                     /locus_tag="PC399_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594444.1"
                     /translation="MVCKIFITLKTILPKTQIIVTTHSPHILQIDSKEEMIVLDMAES
                     DNVYKKELKLGEYGVLGWTNEGLYLH"
     gene            2316..2498
                     /locus_tag="PC399_003644"
     CDS             2316..2498
                     /locus_tag="PC399_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2594445.1"
                     /translation="MFKFYNNATLMFLPGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2569..3414
                     /locus_tag="PC399_003645"
     CDS             2569..3414
                     /locus_tag="PC399_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594446.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNKDPKYNLNTI
                     KKHLN"
     gene            complement(3638..3802)
                     /locus_tag="PC399_003646"
     CDS             complement(3638..3802)
                     /locus_tag="PC399_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594447.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1544 a          513 c          473 g         1554 t
ORIGIN      
        1 atctatagtg ttacttatat tttatatata tggctatttt tcaacatttt acaatatata
       61 acatttattt ttttaacttt tgtcagtaaa atgtcagcaa aacactttat ccacaatttt
      121 tatataagtt ttccacaact tatcaccaat attatccaca taaaaaaggt agcacttata
      181 ttattagcta ccttttattt attaatcttt atatttccag ctttttatcc aacttcctat
      241 ttctattatt ttttcttcag ttatttcttc tccatcatgt aattttgaaa ttaattctga
      301 taaaggattg ttatgtaaac ctctaaaaac tctttttccc tctattttga gatattctct
      361 ctcttttatt aatactgtat agtgtacatg cctcccaatt ctctatgata ttaaaaaacc
      421 ttttttggtt aatctagcta aaagtataat tgtagttgta tgcttccaac catatgtttt
      481 ttctatttca tctgcaacat cttttgatat tacagtttta taaccagtat gccatatgca
      541 tttcataatt tttaattctg tatctggtaa ttttttattt agcataatat attccttctt
      601 tcaatttatg tcacttcttc aaatttatct agcttaaatt ataataggag ctttttagaa
      661 caaaaactat atttaatttt taccaggtac tctacgtgtt tagtctgttt ttaagtgtaa
      721 aataatgttc atatctataa atttatttaa ctttcaaaca atcctgttga ataataacta
      781 attctattgt attattttag ttgtagaata aaactaaatc ggcaaaacta gagaaattta
      841 gtgacgcaaa gctataggga ctaagactta tataaatatc ttatgagtta tgtcagccag
      901 ttgccaaaga gatagtgttc tttttgtttt tatgaaagtt ttttaggggg ataataattt
      961 atatgtttgg aaataaaatg gataaatgta cacatgtatt gactgcttat attagcagtt
     1021 cgtatgatta ttgtaatttt ttagatacac agttagatga ttttatatta gagtacggag
     1081 aaaatgtagt agagtcttgt ttacaccaag taatggtatt ggtaagtaag tataattaga
     1141 tagacattga tttctagaaa acccttttta gttttatcta catttatatt aatatatagc
     1201 aatgtaaaac gtttaaatat atttttaata ttaagaataa ttaaaatgtt aactaaaaaa
     1261 tataattaag tagacatttc tattaatcta tgatacaata gtatttgtgg taatacacaa
     1321 ttaaatcaag tatatcttga tataggttaa taggagtatt tttaatgaat aatggaatag
     1381 tgaaatggtt taataatgaa aaaggattcg gttttatatc aatggaaggt agagatgatg
     1441 tgtttgctca tttctcagct atacaaactt caggatttaa atcattagaa gaaggtcaac
     1501 aagtaagctt tgatatagtt aaaggtgcta gaggtcctca agcagaaaat ataactatat
     1561 tataatatat agatttttcg ggaaagactc tttttagagt ctttttttat tgttaatata
     1621 tgtaagataa tcattggtga agtaaataag gtatattgag taatgttctt gatagtttat
     1681 atgctaatga atttcaaaaa aataagcact cctataaaaa gagtgctctt aatgtaaata
     1741 taaaccttca ttggtccagc ctaatacccc gtattctcca agttttaact cttttttata
     1801 tacattatca ctttcagcca tatctaacac aatcatttct tctttagaat caatttgtaa
     1861 aatatgagga ctgtgtgttg tgacaattat ttgtgttttt gggagaattg tttttaatgt
     1921 aataaaaatt ttacatacca catagttgat ataaaactta agactagcag actcataagc
     1981 aattagttgc ctctctttac ataccacata gttgatataa aacctagagg ctctcagaga
     2041 agttataaga tgtttagtaa ctttacatac cacatagttg atataaaact ccaaaataaa
     2101 cttagcatct ccaatgttta cacatataca atgctcttaa aattgcagtg aacgcgcaat
     2161 attgcaattg ataatactta tcatatattc tcaatacctt atatttcaac tgctaaacct
     2221 caatttatca caaatatcgc tcactgcaaa atttctatat ttttattata tcatagcata
     2281 taattaatat tttcaattaa tttttacatt taattatgtt taaattctat aataatgcaa
     2341 ctttaatgtt tcttcctgga actaatataa aaactatttc agaaagatta ggtcatgcag
     2401 atataaaaat aactatgaat agctactctc agtttttaga agagatggac agagaagcat
     2461 ttgaaaattt gagcaaaata ctatttattg tattttaaag aagatgactt attcaatatt
     2521 tgatgctaga atataattaa taaaaattta attataggtg attgtattat gaatattatt
     2581 aaaagcataa ctttactctt aacattatta attgtgatat taatgacagg ctgttcccca
     2641 agagaaagtg ctagtgaagt tgcaaaaaaa tatattgata agatgaactc tggtgactat
     2701 gaaacagcat atagtttatt aagtaaagat agtaaaaaaa aaattagtat agaaaatctt
     2761 gaagaatttc aaaatatttt agttagtaca aaaagaataa ttagttataa aataggtaaa
     2821 gaaaaaatca taaaaaaata taaacatgat ggaaaagaat ataaaaatgt tgttaaatta
     2881 gaagaaactt ttaatataaa aaaccttctt tataaaaaag atgattctct aaaagtaaat
     2941 agatattttg tagttgaaaa taacaaatac aagctattat tgtatgaaag tttcaaaaaa
     3001 gaatttggta ttaatagtat ggaccttgct caacttaagc ttaatgattt tggactaaaa
     3061 tactttaaag aagttgacct tatactaaaa aaagctattg ctattaatcc tactgattca
     3121 aagctttatt atgcccaagc atgtaattat atgaattttg atggatttcc tggttattca
     3181 gatacaacat ataatgcttt agattcaatt aataaatctt taaagtattt agataaaaat
     3241 gattctgact acaaaaaaaa tgcaagtaat atatataact taaaaggagt tattcttatg
     3301 tttcacaaaa gatttgatga agcacaacaa tatttaaata aagccttata ctttaacaaa
     3361 gataataaag acccaaaata taatctaaat acaataaaaa aacatctaaa ctagatattt
     3421 aatttttgaa tttataaaat atttttttat gtataattca gcactgaatc ataatctcta
     3481 ctaaactaat tacaagttta aattataaag acttatttct atataaatat tactctataa
     3541 caataagttt cttatttaaa ttttaaaata ttatctaaaa atcataaaaa agaaggtata
     3601 aagagctacc ctcaagctca atatacctta taaatatcta aacttcattt tttactaatt
     3661 tttctatatc tttgataata tgttctttag atttttttcc agctatggca tcaaaaattg
     3721 caacagcctt tttctctcca aacttttctg ctacatcatt aaagtctaaa aatctaacac
     3781 tttcttttgg tttaccaaac atataacacc tctcatacat ttttatatat ttattataac
     3841 ttctatagac ttatttttca ccaataaacg ttagacaaat tagtcccttt atttttttaa
     3901 actagcttta ccttcaaaat tcttaacaaa aacagtttat tctactctat aaataaaaat
     3961 agacaattta atttgtagga aactgataat agaatacagt aaataaaact ttgtttaatc
     4021 tatctgaaaa agttatctat taaaactgct atgaatttat aaagaatatt ctagttcata
     4081 aaaa
//
LOCUS       DAMJWS010000160         4009 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMJWS010000160 DAMJWS010000000
VERSION     DAMJWS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.165"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            167..1672
                     /locus_tag="PC399_003647"
     CDS             167..1672
                     /locus_tag="PC399_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2594448.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1786..2484
                     /locus_tag="PC399_003648"
     CDS             1786..2484
                     /locus_tag="PC399_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2594449.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2769..3857
                     /locus_tag="PC399_003649"
     CDS             2769..3857
                     /locus_tag="PC399_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594450.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         1593 a          395 c          641 g         1380 t
ORIGIN      
        1 cgatacaata ataatagaag taataaaaaa aaataattaa tataaaaatg tgtacaaact
       61 aatgaatatt gatatagtaa gctatcatat gaaagagtat gtacacattt tttttactaa
      121 tttttcacaa tatgaacata aataatttac aggaaaggaa tcttttatga aaattcaaaa
      181 tactgagtgg ggatatattg aatggaaaca tacttatgat gaaaataatc caaaacaagc
      241 tatgaatata tacattgcag taactatgcc tggtaagaaa cattttaatc atgttcatta
      301 tggtcaagag cagatgatat atattttaga gggagagggt ttgtatatta taaatggagt
      361 ttggaagcca ttttatcaag gtatgatttt ttatatagaa tctggtagca cacatgaaac
      421 tataaataca ggcgatagag aaataaaaga acttattgtt tcaaataatg tagatgatgt
      481 aggagagagt gaagttatag atattaatcc taataattat ttaaagaaga ccttgataaa
      541 ttatagtgaa agtacattaa atttgtatgc agcagtagaa tcaattcgtg gtcaatttat
      601 agacccgttt aaaattccat taattatata tgatgattca tggaatatag ttttaaaaaa
      661 tccttatttt ccactttttt gttttgaaaa atgtaatcct atgaaatttc ctcaaaattg
      721 tgattgtatg aatcaaaaaa gtagcaatca atttgtatgt gaatatggga taactatata
      781 taatatacca atactgtaca aaagcaatag cataggtgtt ataagaggag ggtatgtact
      841 attatctgat ttgaatttag atacagagca taataattta tatgatattc cagagggagc
      901 tgctagaagt ataaaacgcc tcttgaaaca aatatctaaa aatataataa atttttgctc
      961 ttttaatgat ataagaaagg atttacaaga aaaagagaag actatagcaa gaacatatca
     1021 ttatggagag caacttgaaa tgaatttaaa agtagctcaa gatatggtta ctaatttaag
     1081 aataaatcat cactttttat ttaatacatt aaattcaatg gcaagcatag cactagatga
     1141 tggtagttat gatttgtata gtgcaataat agatttatca agaatgttta gatatacaat
     1201 gaggtctgat ttaagatttg tagaattaga atctgaaata ttatacatta aaaattattt
     1261 gaatttacag aaattacgtt atggagatgc acttaaagta aaatatctta ttccagagaa
     1321 gttatataac ctttctgtgc cttttaactt tattcaacct attgtagaga atgcatttac
     1381 acatggattt agagatattg acacagaaaa gcgaattgaa ataattgcaa gattagattc
     1441 tcagtatgcc attatagaga ttcataataa tggaactatc ttagatggaa ataatataga
     1501 caaaataaag gcaggtattc gtagtaataa tggtcatggt ttgtctttaa tatatacaaa
     1561 attcacatct gcttatggaa ataattttga tatggatata aagtcgagtg ataatgaagg
     1621 aacttacata atgattaaaa ttcctattga aaattataag gagtatgtat agaacttgtg
     1681 aaaaattaat aagttttata aatgttaaga aatatattag atgtttttat gttgttttat
     1741 atatcttact tttttatatt attaagctaa aagggaggta ttctcatgag aagagcacta
     1801 ataatagatg atgaagaagc tgttctaaaa ataataacac attttataga aatgaaagat
     1861 atgccaataa aaattgttgg taaagcaaca tctggtgatg aagcagtaga taaaatcatt
     1921 ggtttagagc cagacatagt ttttatagat attcaaatgc caatatacaa cgggttagaa
     1981 gtaatagaaa aaacatctca tttatctaaa aaaataaact ttgtagtaat aactgcattt
     2041 aactattttg aatatgctca aaaagctttg agattaaatg taaaagatat actccttaaa
     2101 ccattggata tgaaagaatt tactaagtct gtagaaaaaa ttattggata tcaatatacc
     2161 aataatgatt tattaaatga gatactggaa tatataaatc taaactatag caatgactta
     2221 aagttaaatg attgtgctag attattttta accaatccat ctaatatatc taggattttt
     2281 aaaagtaatt taaatacaac ttttatatca tatctaaatc atataagaat agaaaaatct
     2341 attgagttac ttgaaaatac tacaaaaagt ataaatgaga ttgcagaaat agttggatat
     2401 aatagcctaa ataactttta taaaaacttt aagatagaaa aaggaatgac tccaaaagtt
     2461 tataaactga acactaaaaa ttgaaattga taaataaaaa gtcaatatag ataattgttt
     2521 gagtgtgtct gaataagtat actttaatta tgataaaaat taaagcgctt taaacatatt
     2581 taataatatt aaaccttata aaagctgtac ctatttatat aaaaaaatat atataaataa
     2641 aaagaaaaag atatacaaaa taaaaaaagt gaattaaata ctgtttcaaa aagacagaat
     2701 tttcatttaa tttactgaga gtttatattt cttaaaggaa ggtaacttta taatggaggt
     2761 aatttaatat gtcagacaaa gtgaatatga aaaatattat ctgtattgca ggcgcttatc
     2821 tagcaacagc aattggttct gggtttgcta ctggtcaaga aattctacaa ttttttgcct
     2881 cacaagggta tatgggttta attggagcat taatatcaat ggtattgttt tcatttcttg
     2941 gtgctgaagt tataactaga ggtagagaat taaaacttaa agaaccaaca agaatttaca
     3001 ctttttattg tgggaagatt ttaggaaaat tttacgaatg gtttagtcca atatttttat
     3061 ttgggattct tatagtaatg atttcaggtg caggctcaac tttaactgaa tactatggta
     3121 taaatcctta cttaggaaga gtattgatgg ctgttgctac tttaattaca gtgtctatgg
     3181 gtttagaaaa attagtaagc atacttggac gtattggacc agtgatagca gtatttacaa
     3241 ttgctgttgg attaataagc ttgtttaagc attttgacgg tttatctatg gttggagaag
     3301 ctatgaaaaa tattgaagtt acaaaagcat cagaaagtcc aatcgtttct ggtgtaatat
     3361 ataatacatt aaatattatt gtaatgattg tttttttaac aggaatggga gcttctattg
     3421 aaagtaaaaa ggatgctttt tggggaggtt tattaggtgg aatagtgttt atgactgctg
     3481 gtatagttat gtatttggct atgatttcag atataggaaa tttgtatatg aaagaaatac
     3541 catcacttta tttagcagat aatatatctc cgattgtagg tgtatgtttt tcagttgcat
     3601 taatattagg tatctataca acagctgttc cacttttatg gtctgtaacc aatagaattg
     3661 ttgaagatga acatcctaaa tttaaattgg taacaacagt aatagctatt ttggcttgta
     3721 taggaggttt tttaccgttt ggtaaattag taaacatttt atatccatat acaggttata
     3781 tggggctttt aattttagtc tgtatggtgt ataaagcaat tacatgtaga aaaaaatcaa
     3841 cacaaagtca ggaatagagc cacttattaa tagtttttat ttaaaaaaat tttttatata
     3901 atccgaattt aaaaagtcaa aatagttaaa taaattgcaa aacatgataa tatatttaat
     3961 taaaataata tagtaaaata taaatataaa ctaaaattaa atttataaa
//
LOCUS       DAMJWS010000161         3881 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMJWS010000161 DAMJWS010000000
VERSION     DAMJWS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.166"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(171..566)
                     /locus_tag="PC399_003650"
     CDS             complement(171..566)
                     /locus_tag="PC399_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2594451.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            707..1099
                     /locus_tag="PC399_003651"
     CDS             707..1099
                     /locus_tag="PC399_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594452.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1199..1615)
                     /locus_tag="PC399_003652"
     CDS             complement(1199..1615)
                     /locus_tag="PC399_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2594453.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1630..2541)
                     /locus_tag="PC399_003653"
     CDS             complement(1630..2541)
                     /locus_tag="PC399_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2594454.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2723..3745)
                     /gene="splB"
                     /locus_tag="PC399_003654"
     CDS             complement(2723..3745)
                     /gene="splB"
                     /locus_tag="PC399_003654"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2594455.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT         1300 a          661 c          437 g         1483 t
ORIGIN      
        1 gtatgtaatt attcaaaata gtatatcatt attgaaatac acatctaata actaaaaatt
       61 tttacatatt cattatttaa aaatcttttt attgcattta aattaaatct atcaacattt
      121 cagtaataac gttttcttaa ttttcactat ggatgtcgat tcaattataa ttaaagctct
      181 acatcaatct ttttaccact cattgaacaa caactaactt ttgccttatc aatagagaat
      241 atttcaaact taccatctcc tacagagtac atctgaccta aacttggcat tatttcaagt
      301 gccttagctt gtgtttcttt tgatgtaaca aatactgctt cacctaaaat acgtagagta
      361 ttaaggtttt ggtctataca acaaagttca acctttggat taaaaataag ttgcttaaac
      421 atatcctttt gattagatgt gcaaaatgta agtttaccat tgtattccat aacaaatcct
      481 aaaggacgca catgtgcttg attgttctca tcaactgttg ctaaataata aactcgtgcc
      541 ttgttcaaaa attctaatgc tttcataatt cttacacccc ttttaaatac ttgtattaag
      601 gattatcctt tgatataatt ttaatataaa atacagaaaa aacaagtatg cagttttttc
      661 tgataaagta taaaaaagat actactataa ctaaggagta attaaaatga gtataaatac
      721 tgaaaaaaat ataactaaaa acgttgaaga attaacctgt ccaatccgtt atgcattaga
      781 cattgtcggt ggtaagtgga aattacctat tatatgtatg ttagcagttg aaaatccaat
      841 tagatatagt agcataaaaa gaaaattgga tggcattaca aatactatgc tagctcaatc
      901 attaaaagat ttagagtcta ctggtattgt tcatcgtaaa caatataatg aaattcctcc
      961 aaaagttgaa tatacattaa caagtaaagg taaaagtatt gtgcctattt tacaacaatt
     1021 tgctaattgg ggtgctacta atatgcaaga aaaaaacaca tgtggactta gttgcaaaga
     1081 atgtagaaag ataaagtaaa tttaaattta ctaaattatt catattatag actatatatt
     1141 aatttatctg attgaatata aaaacaggat gacttatata ttcatcctgt tctatatgtt
     1201 aagctctaaa tttaattagt tcaagcatat cgtttaatac caataaacta tctacttgaa
     1261 tcattagcca tttacttcca ttaaaaaagt ttacctcatt atatattttt ttgatataat
     1321 cacaacaagt attaataaat aaatcaacct ctactctatt gctttcactt acaataacca
     1381 aagctttaaa ataaccttct tgtggatata aagtacatag tgatttacct ctttttttat
     1441 attttacatt ccaacctctt tgcattgaac atttactgta ttcaagctta ggttctacat
     1501 tataagtaga aaccaaagtt ttatttaact tgtcccataa tggattatta ataaattcat
     1561 taatttgctc aaatgtaggt ttagtatctt gaaaaaacgc atcttgccaa ttcatattca
     1621 tcatcctttc tattgttcaa taggaaccgg aacaatccaa tgttccagat atgctgcttc
     1681 aggtgtagtg gggtaataca tctcaattgc aaaatcagta gttgttaaat tatgtttctt
     1741 catccatctt tccataaatt tatctgcttt aaaaacagct gaattaacta actcattaaa
     1801 gttttctgcc tcaaatccac atactaaata ttctttagct ggcaactcaa aagtactata
     1861 tccttctact gatgtacctg actctacctc tacacctgcc atatacatac aatttccttc
     1921 ttttgcattt cccatataaa gagcagcaaa ttcatttcca cctggaattt gatttggtat
     1981 attatgtctt tgacttccaa gtttcatcca taattcctct actattgcaa ttccagttgt
     2041 ttcaccgccc accaactggc aaataggtac ttctatttct attccaacaa atgtccttgg
     2101 attactcaat gtcttacgat tgacttcaat cactatatca tctgcaatca gtggaacacc
     2161 ttcatcaacc atagcataat ttaaaattaa ttctggctta ataaagtgat tgagttttac
     2221 tggatttgct ctatattttt ctggagttaa tccatatgct tttttaaagg aacgtgtaaa
     2281 tgtctcatga ctagcaaatc caaaatttaa agctacatca agtatacgat tcttatgttc
     2341 tgctaagtat tcagaagctc ttgcaagacg acgaagcttt atatactcca ttacagtctt
     2401 atttaccaat cttttaaata gtctttgata gtaaaatggt gataaatttg ctttatttgc
     2461 taattcatta attgatattt cttctgataa atttgcttct attaaatcca atgtaatttg
     2521 aatagactcc catgcatgca tactataact cctcctgata tttttgttac tcttatcctt
     2581 tatagtatca aaactagaaa aaatgttctt gatagatagt gcccttttta attttcttat
     2641 atgatagtgc taaatctcat tgctttatta taaacataaa atgtataaaa ataaaatata
     2701 gcaaatcttt aggtaagatt tgctatatta tatattttat taaactattt ttaaatactc
     2761 catttatttc cttaagaaag aactctttca tatcatctaa ttgttcttta tcatacacgt
     2821 acttaccata tccaaattga ccgtatttaa actttctatc ctcattttcc attggcaata
     2881 gtgtatctgg ataaatctct agtattctat tttttgcttt agttgtatat ctatgagata
     2941 taacttcaaa tataattttt ttatcaaaat cctttggtaa agcttctttt atacttataa
     3001 tcagattttt atactcttct ttccaacctt catataaaaa cacaggtgct attatgaaac
     3061 caatctgata accagcccta gcaagtttta cagctgactc tattctttta tttgcagagg
     3121 ctgtataatg ctcatgttca tttataattc taggagtatt gatactaaat cgtatttctg
     3181 tatgattatt atgttcagca tccaataagc tatccacatc actgtattta gtaacaaatc
     3241 taaatcttcc tttttcctct cttccaaaaa actctattgt tttctttaaa gaattagtat
     3301 atggctctac tggaactggg tctgatgtag ctgccccttc aaatatggtt acctgtggct
     3361 ttctatcttc aatatatttt tttgtctgtt caagtatttc atctacattt acaaacaccc
     3421 ttataaatgg tttatcacct aattgtgtgt tcaaataaca atactcacat cttcccatac
     3481 atccactgac aagtggtagt tgataattag cagaaggttt acatgtttga aacttcagac
     3541 tttttctaat cccaactacc atagtctgct tcccacttct atattgttca tatagatttt
     3601 cacctggaat atgttcttta atcctatttg atgatatatt aattatctct acatctttat
     3661 tatctctaaa tttattgtat atttttttac ccacatcagt gtctaaagtt cctttttcga
     3721 atattattct tttgggatta aacatttatt tcacctctac ttaaagtttt taaatcgaag
     3781 aattttataa aatattactt aattaatctt ccctttttta aactccttta tttattagaa
     3841 agtaaaataa aagcttaaga caaatttatt atatatataa c
//
LOCUS       DAMJWS010000162         3847 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMJWS010000162 DAMJWS010000000
VERSION     DAMJWS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.167"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(218..1468)
                     /locus_tag="PC399_003655"
     CDS             complement(218..1468)
                     /locus_tag="PC399_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF2594456.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1501..2442)
                     /locus_tag="PC399_003656"
     CDS             complement(1501..2442)
                     /locus_tag="PC399_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF2594457.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2682..3758)
                     /locus_tag="PC399_003657"
     CDS             complement(2682..3758)
                     /locus_tag="PC399_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2594458.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT         1148 a          712 c          416 g         1571 t
ORIGIN      
        1 tttaaaacct tataaatata caaatacaat tatatgaatt ttcatcctca attatagcat
       61 agaatatgac tatctatatc ctaaaattat ccatttctaa aaaaaaggta actattatta
      121 agaatatcaa cttatagaat ttatattcca catacgattt tcaatcaaaa aaacaagggc
      181 taaaaagtga attgtgcact ttttagccct ttattaatta ttcatttatt tcatgtaaga
      241 atagaccaca tcttacttta ggttcaaacc atgttgactt agctggcatt atcttatttg
      301 catctgctac tcttattaaa tcttcaatac tagtaggata cattgcaaat gctactttca
      361 tatcttcatt aactcttttt tctaattctt caagacctct aattccaccg ataaaatcaa
      421 ttcttttatc tactctagga ttatcaattc ctaaaatagc atcaataaca tagtcttgta
      481 atatagaaat gtctaatgag tcaacagggt cttctatttc taataaattt ttatttgctt
      541 tcattttata ccacttatca cctaggtaca ttccgaaagt tccttttttg tcaggtttat
      601 atttttcttt tccttctatt tcttctagtt caaaattttc ttttatttta tttataaatt
      661 cttcctcgct taaaccattt aagtctttga taaccctatt ataatccaaa accattaaat
      721 tctcatctgg tgcaatcatt gctatgaagt aattaaattc ttcttttcca gtataatttg
      781 gatttttagc tctcatttct ttggctacat tttctgctga agctgtccta tgatgaccat
      841 ctgcaatgta tagataatct aatttttcaa atttatctac taggttatct attatatcat
      901 cattatcaat cttccatact gtatgctcta tcttgtcatc tgttatgaaa tcatataaag
      961 gttcattatt atccataaaa tcattgatta tatcatctat caccctttga tgtttgtaag
     1021 ttacaagtat agttcccgta ttagcatcac agtattttat gtgtttagtt ctatcaattt
     1081 ccttttctgg tcgagtgtac tcatgttttt ttattgttcc attcaagctt tcatcaactg
     1141 atatacatgc tactatacca acttgagctc taccatccat gatttgttta tatatatata
     1201 tacactcttt gtcatctttt actagaattt cttgttttcg aaactcatct aaattttctc
     1261 gcgcttttaa ataaactttt tcatcatata aatctatatt ttcatctaaa tcaatttctg
     1321 atttatctat atgcaaaaat gaatatggat tcccttctgc aattcttcta gcttctttac
     1381 tattcataac atcgtaagga agtgctgcta ctttatctac tatatattta tttggtctaa
     1441 tcgccttaaa tggtttaatc gttgccatta ctaatttcct ccctaattat acaaaatatt
     1501 ttataatgct acccctatta gattgttgaa gtcaagaaag aaatcttgaa tcacatttac
     1561 tatttcgtct cctattcttt cttgagcttc tactgtagat gccccaatat gaggtgtagg
     1621 agaaactctt ggatgattta ctaagtctaa atttaatgta ggttcttgtt cataaacatc
     1681 tatagcagct gctgataatt taccttcatc taatgctttt agtaaatctt tttcatcaac
     1741 taaaccgcct cttgcacagt ttattaaata agcccctttc ttcatcttat ttatttcttt
     1801 ttcagttatt actgccccct tattcttatc aaatggtatg tgtaaagata agaaatctgc
     1861 attttctaaa acttcttcca tatcacagaa tttataattg ttaaagcctt gcgcctcacc
     1921 cattatgtca gtatatataa cattcatacc taataattct gctctctttg caacttcttt
     1981 tgcaattctt ccaaaaccta taagtccaag tgtctttcca ttaatttctg tacctttata
     2041 ctttttcttc tcccattttc catctctcat agttacattt gcagtattta tgaatcttgc
     2101 aagaacaaat aattgaccta ttgtaagctc tgcaacagat atactgcttg catttggagt
     2161 attcataact tttatgccat tagcttgagc atatttaaca tctatattat ccagacctac
     2221 tccacctctt actatcaact tcaacttttt accctctaat gctttatcta ttacatcttt
     2281 agtaacttta gtagctgaac gtactacaag tacatcaaca tctttaagtt tatctcctaa
     2341 aacatctttt tcataaaact gctctatcac attaaaatct aactctctca attttctcgc
     2401 tgcttctttc tctattccat cagtaactaa tatattatac ataacaaact cctccaattt
     2461 ttattcactt aatttttcca atttttattt tttaaagtat tttcatcaat aaaaaacttt
     2521 acttaattta actttctgtt tatataattt tcaaaatatt tttttattaa agtcatatca
     2581 aattttccta ttgtttgtta aaaatttaat atgtaatttt tataaaatat tatttattat
     2641 atattctaaa tttatcttaa aacatacata tttttacaat attatttatt aaaacctaat
     2701 atatcatcaa tattatctaa taattcttgt acatcttcga cagtatagtc acccatatgt
     2761 gaaattctaa aagttttttc ttttaaatct ccatagccat ttgctatttg gaatcctcgt
     2821 tcttgtaatt ttgaatttaa atttgataca ctaatacctt gagtattttc tataacagta
     2881 agtgtattgg ataagtgatt ttcattggta aaaatttgga agtgtttttt agcccagttt
     2941 ctaactaagt ttgccatgtc ttcatgtctg ttgaatctgt tatctaggcc ttcatctaat
     3001 atattatcta attggaaatc taaagcgaac atgtgagaaa gagatggtgt tgaagggtat
     3061 tggtagtttt tcttgattaa gtattcatac attgctaaca aatcaagata tactcctcta
     3121 aaaggtactt gcttagctct atcgattgct ttttgagaaa atgtgcaaat agacattcct
     3181 ggtggtaatc ccagtgcttt ttgtactgat gttatacaaa tatctattcc aatcttatca
     3241 acctcgactt ttattccacc ggctgaactt acagtatcta cacagtagat tacatcttca
     3301 tactttttaa caacttctcc aatttcttct attggatttc ttattccagt tgaagtttca
     3361 ttgtgagtta cagttattaa atcgtattct ccggtagaaa gcaccttatc aaccatttct
     3421 ggcgttgttg cctctcctaa ttcaacttta aatatatccg ctgggacatt attagccact
     3481 gccattttgt accatctatc accaaaggat ccacaggaaa atactgctgc ctttttggat
     3541 gtacaggaac gaatggagcc ttccattaaa ccagttccgg atgacgtgga taaaagtatc
     3601 gtgttgtttg tatagaatag tttttgcatc ttttcactta tactcttttg gagcatagat
     3661 gcatcttttc ctctatgtcc tatcattggt gtagccatct tttgcaagac ttcttcttta
     3721 acatcaatag gaccaggaat aaataatttc ttactcatta taattccccc ctttttagtt
     3781 tgtaatatgg atagcgtttt tactgtcctt atttttaata actataatat caagctgaat
     3841 taactat
//
LOCUS       DAMJWS010000163         3692 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMJWS010000163 DAMJWS010000000
VERSION     DAMJWS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.168"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            202..1782
                     /locus_tag="PC399_003658"
     CDS             202..1782
                     /locus_tag="PC399_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF2594459.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1960..2406
                     /locus_tag="PC399_003659"
     CDS             1960..2406
                     /locus_tag="PC399_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF2594460.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2427..3470
                     /locus_tag="PC399_003660"
     CDS             2427..3470
                     /locus_tag="PC399_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF2594461.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1494 a          341 c          693 g         1164 t
ORIGIN      
        1 ttaaatcaac ataataactt aaattatatg gtataataaa ttaaataatt gttttaattc
       61 aaaatttttt gttattttaa attatttcaa aactcttagt tttagtgatt ttatttatga
      121 tatttatggg attaaaaaga tttcaataga tatttttggt aagtatttaa gaagtcttta
      181 actttatagg agtgataatt tatgctaata ggtacatcta aattaagcag aaatgttgat
      241 actgcatgtg tagaagaagt agggattcca atgattgtat tgatggaaaa tgcggttata
      301 tctgcaatga aaaatatgga tatagacatg tataatagtt atacagttgt atgtggtgtt
      361 ggaaataatg gaggagatgg tcttggaata gcaagacatc taaatacata tggaaaagaa
      421 gtaaacgtat ttttagtagg taatcttgaa aaactaagtg aatgctctaa aataaattac
      481 aatgtacttt taaatatggg aattaatatt ataaatatta ataaaaatga tttaagtgaa
      541 aaaaattatg gagaggaaaa tgaagtagat aaaattatta aagttgagaa acaaaatatt
      601 ataaaaaata aaaatttaac aatttttaaa gatagagtat ctaaaagtga tgttgtagta
      661 gatgctatat ttgggactgg attaaaacgt gatataactg gtatttttaa agaagttatt
      721 gacatagtaa acgataatag caaaaatacg tattctatag atattccttc aggtatagat
      781 agcgataatg gaaatgtatt aggaacttgt attagagcaa acaaaacgat tagctttgag
      841 ttttataaaa gaggttttct aaactatgac actagcgcac ttataggtga tgtagttgta
      901 gaaaagatag gtgttccaga ttttattaca agaaaatacc atgatgaaga gttcataact
      961 gataagactt ttgtaaaaaa taatattaga agaagagata agcatggttt taaaagtgat
     1021 tttggaaaag tatctatagt tgcaggttca aaaggatttt atggtgcaag tttcatagca
     1081 actgaatctg ctgttaagag tgggagtgga cttgtaacat taattagtag tgaagatgtg
     1141 caagaaaagg tatctattag acttacagaa gcaatgactt taaattttga agaagaaaga
     1201 ttaaataaat tacttaattc tagtgatgca attggttttg gtccaggtat gggagataat
     1261 agtcaaacat ttgataaatt acttaagata gtagaaaatt ctaattgccc aatagtatta
     1321 gatgcagatg gattgaacgt tatgaaggac agatgttata agtttttaga atggaaaaat
     1381 agatttgtaa ttacacctca tttgggagaa atggccaggt tgacaggaga ttcaattggg
     1441 tatataagag aacatagagt agatgttgca aaagaatttg ctcaaaaata taatctggtg
     1501 gtgcttttaa aaggttatca aactgtcata acagatggta aaaaaacata tataaatcct
     1561 acaggaaata gctgtatggc aactggtgga atgggtgatt gtttgcttgg aatgattact
     1621 tcatttatag gtcaaggtat ggatatattg gaagctacag ttagtggagc atatatacat
     1681 ggctatatag gagataaact gagcaaagca atgtatacgg taaatgctac ggatttaata
     1741 agtaacattt cattaacaat gaaagagttg ttggatatat aattggtgat atttgatact
     1801 actttgtaac aaatttaaca aagtatgttg atataatact attaattaat taattaattt
     1861 attttattgt tttgtaattt aaatttatat tcaaaaacat tgtataaaca tataatttat
     1921 atagggtttt ataattcata tgaaaagagg gtagatagta tgaagttaat atggttgata
     1981 gtagcagtct tatttggaat agcagaaatg ttaacaccaa gcttaacatt gatatggttt
     2041 agtgtaggag ctgtaatact tatattttta agtagcttta tagagagtat tttcttacaa
     2101 attttaatat ttgcagtaat ttctatagca atgttagtcg tagctactaa aaaaatagta
     2161 aaaaaagata aaggctataa atctaacact aatttacaag caatgatgtc taaaaaaggt
     2221 attgttacag aagagatttc tccaaataac acaggtctag ttgtagttga acatgaaaca
     2281 tggacggcta tttctataga tggtgaaaag atagaaaaag gttctacagt agaagtatta
     2341 aaaattgaag gggtaaaatt agttgttaaa aaagtagacg ccacagttaa tgtgacaaac
     2401 caatagaata aaggaggatt tatagtatgg gtactaaaat tgttttaagt attgtattaa
     2461 tagtagtagt cgtagcaata agtttaactt gtataagagt tataaaacaa tctaaagtag
     2521 gtataataat gagacttggt aagtttcaaa aagtagctga aacaggtgta catttcttaa
     2581 taccattttt agataaaatg gcatatgtaa ttgaccttag agaaattgta atagattttc
     2641 cacctcaacc agttataact aaagataatg taactatgca gattgataca gttgtatatt
     2701 ataaagttac agacccagtt agatatgtgt ttgagatagc taatccaata gctgctattg
     2761 aaaatttaac agctacaaca ctaagaaata taattggtga acttgattta gatgaaacat
     2821 taacatcaag agatataata aatgtaaaaa tgagaactat ccttgatgaa gcaacagata
     2881 aatggggtat aaaagtaaat agagtagagt taaaaaatat aatgcctcct caggatattc
     2941 aagttgcaat ggaaaaacaa atgagagcag aaagagaaag aagagaggca atacttcaag
     3001 cagaaggtaa taagtcagct gcaatacttc aagcagaagg tgaaaaacaa tctgcaatat
     3061 taacagcaga agccaaaaaa gaagctatgg tacgtgtagc agaaggtgaa aaagaatctg
     3121 ctatattagt ggcagaaggt gaagctgaag ctataagaca gacagctatt gctaaggcac
     3181 aaggtgaagc tgaaatgata aaaagaactc aaatggcaac agcagaaggt ttaaaattag
     3241 ttttttcagc aatgaaagag gctgatatag acaataatat tctagcatta aaatctatgg
     3301 aagctcttga aaaaatggct gaaggtaagt caacaaaact ggttttacct tcagaagcag
     3361 ttaatttcct aggaacattc aaaggaataa aggaagttat gagtgatgat aacaaagaag
     3421 tacttgatat aaaagaagtt ttaaatgata atgaatcatt aaaaaaataa aagagttttg
     3481 ttatcatagg aaggatatgg aaattaaagg tcgaattaat taaatatagc acaaaaatgc
     3541 acgttgaaaa gaagaggact tataaatttt taagtcttct tctttgtttt tattagaaag
     3601 atgaaattat aaaaataaat taacagaatc tttatacaat ttatccttta ctaatgtaaa
     3661 aagtgttata taatataata taaaacatat ag
//
LOCUS       DAMJWS010000164         3686 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMJWS010000164 DAMJWS010000000
VERSION     DAMJWS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.169"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            261..620
                     /locus_tag="PC399_003661"
     CDS             261..620
                     /locus_tag="PC399_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594462.1"
                     /translation="MKLRESYTCPLELVHDIIKGKWKTIIIFQLRNGKCSFSELHHSI
                     AGITQKMLLEQLRELKHFGLIDKKTYDGYPLKVEYFLTGQGEKMLSAIKIMQEIGIDY
                     MVEHGMTEILDEKGICY"
     gene            710..1261
                     /locus_tag="PC399_003662"
     CDS             710..1261
                     /locus_tag="PC399_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2594463.1"
                     /translation="MKKALLIIDVQNDYFPNGKCQLYKPEIALNTIKDLLKIFRKQNL
                     PIIYIRHESIHGTFFNPNTNGVQIHNDIKPLDTETVIVKHYPNSFYETNLQNKLVEIE
                     VTKLVVCGMMTHMCIDTTIRAAKDYGYEITLISNGCATKELKWNGVNIPADTVQSIFM
                     ASLNQKFANVITSTEFLSNEITR"
     gene            1245..1610
                     /locus_tag="PC399_003663"
     CDS             1245..1610
                     /locus_tag="PC399_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594464.1"
                     /translation="MKLRDEYTCPLELIHDMIKGKWKPIILWRLRLGATSLSKLENDI
                     DGITQKMLLEHLKELIDYGFVDKKTFSGYPLHVEYFLTEDMGNQILEALRIMQHIGIE
                     YLRKNGMENELTEKHVIPD"
     gene            1698..2198
                     /locus_tag="PC399_003664"
     CDS             1698..2198
                     /locus_tag="PC399_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2594465.1"
                     /translation="MYISSIGISNGIIEKKYGSHGVQFNENDVPTYSIPFKIENAPDK
                     TVSFAFILEDKDAYPVTGFIWVHWLGANLTRSELKENESQTAKDFIQGANSWTSILGN
                     QQTREQTSCYGGMAPPDKAHIYELHVFALDKLLDLKNGFYLNELYQEMEGHILEEAVL
                     KGLYEK"
     gene            2188..2703
                     /locus_tag="PC399_003665"
     CDS             2188..2703
                     /locus_tag="PC399_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2594466.1"
                     /translation="MKSKPVKVGLRTIKTVLSAFLCFLIDSLRASSVPFYAVIASILC
                     IQPDMENSLSVAKNRELSTIIGGILGMLFLLMERIFLPIQPEILRELTLSILLIPIIN
                     LSVLLKQKKGTFLTCVVFLCVTVTHEKDISPIWFAWNRIVDTSLGIGISLAVNSFPFH
                     KFKVGGTMGKY"
     gene            2697..2978
                     /locus_tag="PC399_003666"
     CDS             2697..2978
                     /locus_tag="PC399_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2594467.1"
                     /translation="MLIVEFTEKEDAVFQQAVEWLSSKLAYTPASFNQQKKGFDIDPL
                     TRTFTNEEGTSIYLTAKEFDLLYFLSSHKGQVFTKEQIYEKVWGYDNFY"
     gene            3132..3322
                     /locus_tag="PC399_003667"
                     /pseudo
     CDS             3132..3322
                     /locus_tag="PC399_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            3346..3570
                     /locus_tag="PC399_003668"
     CDS             3346..3570
                     /locus_tag="PC399_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594468.1"
                     /translation="MIGIKKRDIKKALKAGAKAGGSSYADVLADIEATIDEVMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTRV"
     gene            3592..>3686
                     /locus_tag="PC399_003669"
     CDS             3592..>3686
                     /locus_tag="PC399_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2594469.1"
                     /translation="MKNEMWVLCPICNNKTRTKVREDTELINFPL"
BASE COUNT         1329 a          501 c          721 g         1135 t
ORIGIN      
        1 ttccccatag aataccttat cgcctaaatc attacgagaa agcccagctt tttcacgggc
       61 ttctctgatt gccagcccta gcggtctgaa atcaaaagta tgtgtttctt ttgtttttct
      121 cattttaaat caccatacat gagtttatac ttcatggttc gtatttagaa tgtgttaaaa
      181 gtgcatatat tagggtaata aaatatgttt tgaaatattg agcttaaaca tgtaagatat
      241 ttcacatagg agatgataaa atgaaactta gagaaagcta tacatgtcca ttagaacttg
      301 ttcatgatat tataaaggga aaatggaaaa cgataattat ttttcaactt agaaatggaa
      361 aatgctcttt ttccgaactc catcacagta tagcaggaat tactcaaaaa atgctattgg
      421 aacaactgcg tgaattaaaa cactttggct taattgataa aaaaacatat gatggatatc
      481 ctctaaaagt agaatacttt ttaactggac agggagaaaa gatgctatca gcaattaaga
      541 taatgcaaga aattggtata gattacatgg tagagcatgg tatgacagaa atccttgatg
      601 aaaaaggaat ttgttattaa actctatcat atacacgaaa aagtaagtaa tttacttaga
      661 aactattatc ttatattatt taattagaat taagagagga tatgagagta tgaaaaaagc
      721 attacttatt attgatgtgc agaatgatta ttttcctaat ggaaagtgcc agttgtataa
      781 accagagatt gcgttgaata caataaaaga tttattgaaa atttttagaa agcaaaactt
      841 gccaattatt tacattcgcc atgagtctat ccatggaaca ttttttaatc caaatacaaa
      901 tggtgtacaa atccataatg atattaaacc attagataca gaaacagtta ttgttaaaca
      961 ttatccaaat agtttttatg aaacgaattt gcaaaacaaa ctggtagaga ttgaggtaac
     1021 gaaattggtt gtttgtggaa tgatgacaca tatgtgtatt gacacaacta taagagctgc
     1081 caaagattat ggctatgaga ttacactaat atcaaatggt tgtgcgacca aggaattaaa
     1141 atggaatgga gtcaatatcc cagcagatac agttcaaagc atatttatgg cttctttaaa
     1201 tcagaaattt gctaatgtta ttacaagcac ggagtttttg tcaaatgaaa ttacgagatg
     1261 aatatacatg tcctttggaa ttaattcatg atatgataaa gggaaaatgg aagccaatta
     1321 ttttatggcg cttacgatta ggtgctactt cattatctaa attagagaat gatattgatg
     1381 gaattacaca aaaaatgctt ttggaacatt taaaggaact tatagattat ggatttgttg
     1441 ataaaaaaac attttctggt tatccacttc atgtcgagta ttttttgacg gaagatatgg
     1501 gaaatcaaat actagaagca ttacgaatta tgcaacatat agggattgaa tatttaagga
     1561 aaaatggaat ggaaaatgaa ttaactgaga aacacgtgat tccagactaa tacctacaaa
     1621 aaagtgggta atttacgttt tgcttcctct ttgttaatat atatctgtaa ttaaacgata
     1681 tacaaggagg aaaatttatg tatatttcaa gtataggaat ttctaatgga attattgaaa
     1741 aaaaatatgg tagtcatgga gtgcaattca atgaaaatga tgtgccaacg tattcaattc
     1801 cgttcaaaat tgaaaatgca ccagataaaa cggtttcctt tgcctttata ctggaagata
     1861 aagacgcata tccagtgacg ggttttatat gggtgcattg gttaggagct aatctaacaa
     1921 gaagtgaatt aaaggaaaac gaaagccaga cagcaaagga ctttatacaa ggtgcgaata
     1981 gctggacaag catattgggg aatcagcaaa ccagagaaca gacaagctgt tatggtggaa
     2041 tggcaccacc agataaagca catatttatg agcttcatgt atttgccctt gataaactgc
     2101 ttgatttaaa aaacggattt tatcttaatg aactctatca agaaatggag gggcacatcc
     2161 ttgaagaagc tgtactaaaa gggttgtatg aaaagtaagc ctgttaaagt tggattgcgg
     2221 acaattaaga cggtactttc tgcgttcctt tgttttttga tagatagctt aagagcttca
     2281 agtgttcctt tttatgcagt gattgcgtct atactgtgca tacagccaga tatggaaaac
     2341 agtttgagcg ttgctaaaaa cagggaactt tcaacaatta tcggcggtat actgggaatg
     2401 ctctttttgc ttatggaaag aatttttttg cccattcagc cagaaatact tcgggaattg
     2461 actttatcta ttttattgat tccaatcatc aatctgtctg tactgttgaa acagaaaaaa
     2521 ggaacatttt taacgtgcgt tgtattctta tgcgtaacag taacacatga gaaagatata
     2581 agtcctattt ggttcgcatg gaataggatt gttgacactt cattagggat tggtatttca
     2641 ctggcagtaa actctttccc ctttcataaa tttaaggtag gtggtactat gggaaaatac
     2701 tgattgttga atttactgaa aaagaggacg ctgttttcca acaagcggtg gaatggctgt
     2761 cctctaaact tgcttacacc cctgcttctt ttaatcagca gaaaaaagga tttgatatag
     2821 acccattaac ccgtaccttt accaacgaag agggaacgtc tatatatctg actgctaaag
     2881 aatttgacct actatatttc ctttccagcc acaaaggaca ggtgttcaca aaagaacaga
     2941 tttatgaaaa ggtatgggga tatgacaatt tttattagtc tggcaataat gaagaaaaaa
     3001 gaattttaga aaagtgaggt gtgcttatgg atttatctat tttggtagta gtattgatat
     3061 tgggtgtggg tattccttta agaaataagg caatcaaaaa atacagataa aatatgaaaa
     3121 agaaaggaaa aatgaaaaaa tatgaatatt tagaaattga ttatactgcg aaaggtgttg
     3181 tattcctttg caccgataaa cataaggaag ttataaatag ctatgcagaa aatgggtatc
     3241 gctatgcggg ttcgtgccta cagaaattga cgcaaaaggc tgtatgcgaa agatagattt
     3301 aatttttgaa aaagaagatt aacgtatttt agggagttgg ttcttgtgat aggaattaaa
     3361 aaacgagata ttaaaaaagc attgaaagct ggtgcaaagg ctggtggttc ttcgtatgct
     3421 gatgttttgg ctgatattga agcaacgatt gatgaagtta tgaacagcac cgacccagaa
     3481 gtgcaggcaa atttcaaaaa gtattttgga aataaacgcc ctacaccaga ggaatatatc
     3541 tataaaatca cgaagaaaac acgagtttaa agaattagta agggtggttc tgtgaaaaat
     3601 gaaatgtggg tattatgtcc tatttgcaat aataagacac gaactaaagt gcgagaagat
     3661 acggaattga taaattttcc tttgtt
//
LOCUS       DAMJWS010000165         3684 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMJWS010000165 DAMJWS010000000
VERSION     DAMJWS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.170"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            259..1635
                     /locus_tag="PC399_003670"
     CDS             259..1635
                     /locus_tag="PC399_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF2594470.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1922..2593
                     /locus_tag="PC399_003671"
     CDS             1922..2593
                     /locus_tag="PC399_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF2594471.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2782..3579
                     /locus_tag="PC399_003672"
     CDS             2782..3579
                     /locus_tag="PC399_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF2594472.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDVNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1403 a          410 c          677 g         1194 t
ORIGIN      
        1 ttaagtacaa aatagtaaaa gctatgatag ttttagacct attatatgta aaaaatatag
       61 atgtttttgg tgcaatttaa attaaaagat atatataaaa atttaaaata ttccaaacag
      121 aaattataaa aattactatc attgtcttta tacaatattt atactataat agaataataa
      181 tttagaggaa agctctaaaa ataaacaaat taagggtctt aacagtagtg attttatgtt
      241 aaggaggtat aaagaaagtt gaaaacaata gttagaagac ttattctcta cgcagatgga
      301 atgcgtccta ctcaaataat ggtatctggg tttgctgcga ttattgtaat cggagcattg
      361 ttgttaacac tcccgatagc gtcgcagagt ggagagagta taggcttatt aaatgctcta
      421 tttacggcta cttcagcggt ttgtgttact ggtctagtta tggtagatac agcaacttat
      481 tggagtttat ttggacagat agttataata acattgatac aaattggtgg tcttggattt
      541 atgaccgtag ccacaatgtt ttcacttatg gcacgaaaaa aaatacaact aagagaaaga
      601 ttattaatac aagagtcatt aaatcaagct gatttatcgg gacttgtaag acttacaaga
      661 tttgtattaa taataaccat aacaatagag ggaataggtg cactggtttt atcaactgtc
      721 tttattccac aatttggttt gtctaaaggg atatggtata gtgtatttca cgcaatatca
      781 gcgttttgta atgccggatt tgacttgatg ggcagtgtaa gtggcccatt tacatcgtta
      841 aattcttatg taaacaactt tacagtatca atgactgttt gcgcacttat agtgcttgga
      901 gggctaggat tccctgttgt acttgatatt gtaagaaaaa gaagattttc aaaattaaat
      961 gtgcattcta aggttgtatt gttttcaact gctacactta tttttgtggg tgcattattt
     1021 atttttctta tagaatttaa tcaaaaagct actatggcgg acttgccttt aaagggaaaa
     1081 gtactatcag caatatttca atcagtaaca gcaagaactg ctggttttaa tacgcttgat
     1141 ttagctactt tacgagaaag tagtgtattt gtaatgataa ttttgatgtt tattggagca
     1201 tcacctgcat caactggtgg gggtataaaa acaacaacac tagctgtttt aatcattaca
     1261 gttagaagtt ttctatcagg aaaatcagat atagaagctt ttgaaagaag attagctcca
     1321 tcaactataa aaaaatctct tggtatattt gtaatcagta tatcagcagt tatttttgga
     1381 acattgatta tttcaataac tcagcctaat tttacattag ttcaatcagc ttttgaggtt
     1441 acatcagcac ttgctacagt tggttcaagt ttagctggaa ctccaaactt aaatgcttta
     1501 ggtaagatta taataataat ctttatgttt atgggtagag ttggttctct tactttattt
     1561 atggctatac tttctggtgg tagaagaaag agtcaaccaa tcagatatgc agaaggtaaa
     1621 attatggttg gttaagttac caaggagtaa tataaattta taatggattt agagtagcac
     1681 tgtaaatctg ttgataaaat aaatgagcct agatgtaatt atatgaagaa ataaaatcga
     1741 ctaacgaaca aaattaatta atgaaataaa aaaacaggga aataaaatac aaataaaaag
     1801 gtaaatcaaa attaaattaa tctgtgtata taaaattata tattgtgtat aagttttgta
     1861 tgtgtaaaca attaatttaa gagatattat ataaattgtg tattagggag gaagtttcga
     1921 gatgaagcaa tatatagtca ttgggtgtgg gagatttgga agttcagttg cgtctactat
     1981 gcatctttta ggacatcaag taatggcaat agacaaaaat gaagattcag ttcaaagtat
     2041 atctgacaag gtaacccatt cacttatagt ggatgttact gatgagcaag cgttaaggtc
     2101 attaggttta ggtaactttg atgtagcagt agttgcaata ggttctgata taagggcatc
     2161 tataatggcg actcttatag ccaaagaaat gggtgtagag ttgataatat gtaaggcaaa
     2221 ggatgaatta caagctaaag tgctttataa aattggcgca gatagagttg tatttccaga
     2281 aagagatatg ggagtaagag ttgcacacaa tttagtttca gataatatat tagaccatat
     2341 tgaacttgac ccagagtatt caattgttga aatcgtaact ccaaatagtt gggttggcaa
     2401 gacacttata gagcttgaat taagagctag atatgagata actgtacttg ctataaaaac
     2461 aggtaaaaat ataaatgtta caccttctcc agatgaggaa cttacagccg gaagtatcct
     2521 agttataatc ggtcaaaata ctagtataac agcgataaca tctggaaata aggggataat
     2581 tagaagaaga taatttacta tttaatatat atttaattgc aatgaaagta gatagtatca
     2641 tataattatg aatagttata tgatactatt tttttaatcg aaggtagtat ttatttgtaa
     2701 gattagataa agagaaatta aattaaaagt aaggaggctg tgctaatata aaaatttata
     2761 agttattagc atttagataa aatgattaca aatataaaca gtaaggataa tgaaaagtta
     2821 aagtatacaa gagcactatt aaaatcaaag aataggaata aagagtcaaa gttcataata
     2881 gagggataca gaatagtaat gcttgcactt gaatgtatgg caaaccttga ttatgtattt
     2941 atcaatgaag aatttgaaaa taagaaagaa catgtaaaac tattagaaga tttggataaa
     3001 aaaaacacaa agatatacaa gactactaat aaaaacttta aagaattagt ggatacagaa
     3061 aatactcaag gaataatagg tgtagtttca tttaagaaaa aaaaattaag tgaaagtata
     3121 aataaaaaag ataaatttgt attgatttta gacagaatac aagacccagg aaatatgggg
     3181 actataataa ggactgctga ttctgctgga gtagatgcca taatagcact aaagggatgt
     3241 gtcgatatat acaacccaaa agtaattagg tctactatgg gttctatttt tgatgtgaat
     3301 ataattgatg cttcacaaga tgaaactgtg gacatgctta aatcattgga ttttaatata
     3361 gtttcaagtt acttaaatac agaaaatttt tatgacaaaa tagattatgg ctcaaaagta
     3421 gcattggtga taggaaacga agcaaatgga ataaatgaag aacttgtatc aaagtctgat
     3481 attttggtta agatacctat atatggtaaa gccgagtcgt taaatgctgc gataagctct
     3541 gctatactga tgtatgaaat aaaaaaatac ttaatttaat gtattgtaat aaatataatg
     3601 tcatgttata atcttgaatt aaatagtaga gtatcaaaaa tagtcaatat atattaaaaa
     3661 aataattaaa ttaattatat atta
//
LOCUS       DAMJWS010000166         3501 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMJWS010000166 DAMJWS010000000
VERSION     DAMJWS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.171"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(2..1132)
                     /locus_tag="PC399_003673"
     CDS             complement(2..1132)
                     /locus_tag="PC399_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2594473.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            complement(1519..3456)
                     /gene="htpG"
                     /locus_tag="PC399_003674"
     CDS             complement(1519..3456)
                     /gene="htpG"
                     /locus_tag="PC399_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2594474.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
BASE COUNT         1093 a          625 c          344 g         1439 t
ORIGIN      
        1 attataaaga cttatggtac atttccttta tttgttcctt attaaaatac tttggatgtc
       61 tcttaatatt tgcaactgat attttcatac aattatcagt taaccaatct atatctgatt
      121 tttcaatacc taaatcactt agttttatat ctaaatctat ttttttaagg aaatttctaa
      181 ttgcatctga acaatcttct gcacaagttc ctcctaatac ctttgaaata ttaccaaatc
      241 tttcaatatc actatcccat gaactttcta taatagtagg agtcaaagct gcaagtcctt
      301 ttccatgcat tacatctcta agaccactta caggatgctc cattccatgt ggcaaggctg
      361 tacccgctgt accaatcgtc attcctccaa tagtacttgc aagtgttatt ttttcccatg
      421 cttctaaatc attaacatca ttatatacct ttattaggtt ttctgatata agttcaatcc
      481 cataaatagc ttgtacatct gcaagagggt ttcttccatt agaaagatat gcttccatat
      541 tatgggcaag tgcatcaaat ccaactgatg caattatatg ttttggcatt gtaaccataa
      601 gttctgggtc aattattgat gcttttgcat acatactatt cttctttata gattttttat
      661 ctttagtttc tgaatttgta agtactgcaa aacaatttcc ttcactacct gtacctgatg
      721 ttgttggaac taagattata ggtaaagctt cttcaccttg tctaattcca aatatataat
      781 catttaaatc tcctgggttt ttataagaaa aagctatact cttggcagca tccataatgc
      841 ttccaccacc aagacctaaa acacaatcac atccagttga ttttataatt tctactcctg
      901 aatatatagt agttataaga ggattttgtt ctactctatc aaaaacttca tattctattt
      961 tagagtcttt tagtaaatct attgttttat caagtaatcc agttttcttt gtactatttt
     1021 tacctgttac tatcaatact tttttgccat atttagatac ttcttttccc acatcattaa
     1081 ttctgcctct cccaaataag atatttactg gcaaattata attaaaattc attctaaaac
     1141 ctcctatttc tactttagta ataattttat gtttttaaca aagtgtaatt atgtatttaa
     1201 acataaacta aaaaacaatc ttttacttaa ttatatgtat tatcttatta tatatcaagt
     1261 taactatatt taactaaata gagtttttta ttagaaccta cttaaatatt taaaattaac
     1321 caaagataaa aaaataaaga atggtctgtc tcaaaataga gataaatata tttttcattg
     1381 ttgaaatgat taaaataaat ttaatttcac ataaaaaaag cgcctcatga actgaaaagt
     1441 tcattttgag acacttcttt cttatcactg atattgaata ctttttataa atttgcttca
     1501 atgtattcaa attcaatctt ataattctat caaaagactc ataagcttat tacttctttg
     1561 tacaaatgaa tctaactcat ctggctttaa ttctttatta gaaagtaaag ctaaatctgc
     1621 tatctggtta caaataagcg taatcttatc tttcttttcc tcatcatctt taagagaaac
     1681 caattttttg attattggac tgttctcatt tattacaaga gttttttctt cctcaaaatt
     1741 cattcctaaa tccattccag caaatcttga ctgcatttct gccattctaa tagattgttc
     1801 tgatactaat accatagctg gagtgtcttc atttttcaat ccttctacag aataattctt
     1861 cactctttct ccgacagctt ctttaaacag accttctatc ttagctatct cttctttgtt
     1921 ttcttcacta tctttatttt catttttatc ttttaataca tcagaaaggt ctgcatctat
     1981 tctattaaac ttaactccat tctctttata ttccataaat gaaataaaat gagtatctat
     2041 tgatgagttt aaaacaacag cacttaagtc gtattcttta aatagtttaa tgtattgaga
     2101 ttgctgttcc ttatcactta cataaaatac cttattttca tgtttgtcct tacatttttc
     2161 taaataatct tgtaatgtta tatactcatc atcaatagtt ttgaataata tacattcttt
     2221 taccttctca tagaaacttt catcttttaa gcaaccatat ttaataaata tttgtatatc
     2281 atcccaaaac ttattgtatt cttctctttc atttttacaa agagatttca acttatcagc
     2341 tactttttta attatatgtt tagaaatttt gcttacatcc ctgtcatttt gtaagaaact
     2401 tcttgataca tttaatggta agtctgggca gtctataact ccttttaata aaagtaaaaa
     2461 ttctggtata acttctttta tattgtcagc cacaaacact tgattgttgt ataattttac
     2521 ttttccttca gtcagttcaa actcattttt taatttaggg aagtaaagta taccctttaa
     2581 attgaaagga taatctacat ttaaatgtat ccaaaataaa ggttcatcaa atacattgaa
     2641 tacctttcta taaaactctt tatactcctc atcagtacag tcttttggag attttaacca
     2701 tagaggatta gtgtcgttta aaggctcaat tacttttgca tctacaatct cttcttttcc
     2761 atcttccttt tgttttttag ctttttcttc tgcttcttta gcttctcttt ctaatctttc
     2821 aacatcttct aaatatattt ctactggtaa gaaagaacag tatttattta ttattcctct
     2881 tacagtaaat tcatctaaaa attctttact atcatcatca ataaatagtg ttatagtagt
     2941 tcctctatca tttctagcat ctgattcaga tatttcgtat tctgtaccac cttcagatat
     3001 ccatcttaca ggagtcgctc cttctgtata tgaaagtgta tctatttgta cctttttaga
     3061 caccataaat gcagaataaa atcctaatcc aaaatgacct attatatcat ttgactcttc
     3121 cattttatcc ttatatttat taaagaaatc ttcagcacct gaaaaagcaa cttgatttat
     3181 gtattttttt atctcttctt cagtcatacc aattccattg tcaataaatt taagtgtacc
     3241 ttctccttta tttacagata ctgtaatttt ataatctgat gacttatttt ctgatatttc
     3301 tcctagtgat acaagtcttt tatgtttact tactgcatca catccattac ttataagctc
     3361 tcttataaat atgtctttat cagagtataa ccatttttta attataggaa aaatattttc
     3421 tgtatgaatt gaaatactcc ctttttcaaa ttccattttt tatccctcct cattttataa
     3481 ttaatatttt aaatttttta a
//
LOCUS       DAMJWS010000167         3458 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMJWS010000167 DAMJWS010000000
VERSION     DAMJWS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.172"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(114..1681)
                     /locus_tag="PC399_003675"
                     /pseudo
     CDS             complement(114..1681)
                     /locus_tag="PC399_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(1656..1799)
                     /locus_tag="PC399_003676"
     CDS             complement(1656..1799)
                     /locus_tag="PC399_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594475.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(2017..2199)
                     /locus_tag="PC399_003677"
     CDS             complement(2017..2199)
                     /locus_tag="PC399_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594476.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
     gene            complement(2588..3334)
                     /gene="cdtR"
                     /locus_tag="PC399_003678"
     CDS             complement(2588..3334)
                     /gene="cdtR"
                     /locus_tag="PC399_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF2594477.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         1151 a          451 c          378 g         1478 t
ORIGIN      
        1 tatattagaa gaagataata tttaaataaa aaatacctta taaagttaat tttttaatac
       61 tttataaggt aaatacatat tgtattgtgg ggataaattt aaatcatttg atactaatca
      121 acactaagaa ccaataactc tctatcatct tgagtaattg tatatattct taatttcttg
      181 tatgtcataa tattttctcc acctgtaaaa taactcctaa gattaacaca atcagtttta
      241 ggttgatttg tatctcctaa ataattacgc aattctttat ctttagtttc aatatcttta
      301 aatccaaaat attgtaaagt agcttcaact ctatattttt ggacataatc gagagcttct
      361 ttattagttt atgttggtgt aaaatacata ccatttatat aagcctttat tgtacttgga
      421 ttaaatttaa aatctgcaga atatatttta tgtgtaacta ttatttcttg aacagttgga
      481 attttaactt ctggttcatc aagtatttca ggagtactat ttagctctgt aatagataag
      541 ttatctaagt atattgtatc actatcaatt aatgctatat ttatattaga agaatctttt
      601 tcagtagttt caaattcata actaaatttt gtatatcctt gttctagtac caaataatcc
      661 gttttttctt cttttacttt tatatttaca attattgaat tatatgttaa aggatttttt
      721 gaattttact aaaaacataa cgtttataag gttttagttc agacataggt attttaatct
      781 tcgtctcacc atttaatcta tttgctgagc cttttaaacc atcttgattt gtagtattaa
      841 cattactcca tatactaggg taattattgt aatcatcaaa attagtaaag taagttggtg
      901 tttttataag tatattcatt cctctttcaa gtttaacatt acatatttgt ttatcagaca
      961 aagtttttaa actgtcttta atcttattgc ctatattatc atcaaatatg agttcaacac
     1021 aactttcatc tattggtata ttattaaaat ataacaaacc atctttttta gtagtgccaa
     1081 aagttttttc aattgcttct ccaattgtaa gttcaggtgt tttatcttct gggacctgta
     1141 aacttttagc agtaattctt cttttgtaag attcattttc tgtatctaat ataatagata
     1201 cagaaatact gtcaatttga cctatatcgt cttaccaact atttcattct gttactattt
     1261 gtccagaacc attttttgta ccaaaatttc cacttacttg tgttgtttct aatttaattt
     1321 gctttccatc atctaatttt tttaattaat tataatttat aggaatcagt ctagaactaa
     1381 attgatacat tgtgttaaga gccataggtg aaaatccttt tttgagataa gtatttccag
     1441 gagatagatt attgccaatt tgattttctt gtgctttgat agttgataat gcatctccat
     1501 ctaacactaa atttgttgtt ggtgtcactt tgtacatagg tgcagtacct gtgttgtagt
     1561 atctaatatt tgcatttata tatgcagatt ctcctttgtt tatacttaat ccagtatttc
     1621 atgattatcc attgctattt tgaacagcag ttgaattatc tgttgtatgg taataatttg
     1681 tcttgtatag tttctttaaa ctcattaaat ttttctaatg aaatttcgtt ttgatttttt
     1741 gttcttatta ctttattaaa tgcaaatttt tctggagatt caaaataata tacatacata
     1801 ggtttatcta ttttattttt tgatatagca tttcgaagtt ctctatattc tttttctcaa
     1861 gaatttgctt ctatttgata atcataaaaa taatttcttg tctgagaata tttgcttatc
     1921 tctatagaat cttttttata tgatcctaat acttcttttt cagatttttc aagtttttga
     1981 tcttttcttt ctcattcttt agccttttct ttatctttaa aaaaatcctt ctgatctttc
     2041 tataggagcc ttgtaagtag tattacaaac ttttccacta tagctttgta aatcttgtgc
     2101 ataaatgtta ttaggtaaca aactacctaa agttagatat aatatcaaca atatagatat
     2161 acaattacta atacttttat gtttcctaaa ttttttcatt tattctccct acaaatatta
     2221 gtttagttaa cttttgaatt ttaatttatt tagattatat aatctaaata agaatttttt
     2281 atataaattt cataaaatat gtaggtatat atcataaaaa tatgtattta tatgatatgt
     2341 cagtacaaaa taacaactga atattttaaa gagactattt cgtactttta tgatttaatg
     2401 agctatctta tcgaatcata atctaggaac aattgttata atatctaata gtagttatat
     2461 atgatatagt cagtatgtgt tttttcacaa tatgattatt atatagagga ttcttaggga
     2521 ttactctttt tgtataaaaa agagatggtt caaattttta tcttgagaca tctccttttt
     2581 ctatttatta tgttttaata atattcttta aaatatttct atatgtatta gatatacgac
     2641 atatatggcc attactcatt ttaatattat ttttctttag gtcgagagaa acaatatgat
     2701 atttattaac tacaaaagat ttatgtacac ggataaagca tgcatctaaa tctggtaaag
     2761 actttaaact tttatagaat tcaaatattc tagaagtagt cactaatttt attttatgtg
     2821 gaatagtgct tgtttcaaaa tatataactt catcaaatga aattttccat agtgagaaat
     2881 ctgaatagat agtaaaaaaa ttagagcgat aatttttttc tttaatgtat cttttgtgag
     2941 caattttaat acaagctttt attttacttt cagcaatttc catatcatgt tttagtataa
     3001 agtccataac ttctaaccta taatcgaaga tccctaaaat catttctata tgatttgtaa
     3061 gaaatatgat tggactaata taatctgatt ctctaatttt ttgtgctatc cataatccat
     3121 cacataattc attacttatc ttatattttg cgtctacaac tataaaatat attcttactt
     3181 tatttttttc agtaagctct tttaataaca aatttgcatt atgatataat cttacaacat
     3241 cattatcaaa accttctttt attaaaatat tatttatttt ttcttttagt ttcattccaa
     3301 aacaaacatc attatcaaag attaatatat ccactaaata ccctcctata aaaaattcaa
     3361 aagtaaaaaa acaaaagtat tttatagaaa atacataaaa taacactagg agataaataa
     3421 gattaaggat agttagaatt aaggtatatt tatgcttg
//
LOCUS       DAMJWS010000168         3378 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMJWS010000168 DAMJWS010000000
VERSION     DAMJWS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.173"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(88..453)
                     /locus_tag="PC399_003679"
                     /pseudo
     CDS             complement(88..453)
                     /locus_tag="PC399_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(474..1553)
                     /locus_tag="PC399_003680"
     CDS             complement(474..1553)
                     /locus_tag="PC399_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF2594478.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            complement(1607..2896)
                     /locus_tag="PC399_003681"
     CDS             complement(1607..2896)
                     /locus_tag="PC399_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2594479.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     misc_binding    complement(3059..3270)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1112 a          677 c          372 g         1217 t
ORIGIN      
        1 tatctctaca tataggtttc cctatatgct gactatttta gctactaact catgactaaa
       61 gtcacgagtg tgcgtagcta ttttttaatc aaactcatat ccaccagata ccaatattga
      121 atctaattct attttttctc ttaagtcttc tggccaatct ataaatggaa gaatggcagt
      181 agtggctggc tttcctattc ttatcatagc cacattatta agtctttttc tttctacaat
      241 agcatttgta caatgagttg taccaagcat agcatatttt attaatgaat ggtcaatctt
      301 actttcatct ataacttttt ttaaggcatt gtaaacacca gtttcaacat cttttgtagt
      361 tgccacctta actgaatgaa ttacttttaa ttccccatcc agtatacaag catctgtatt
      421 tgttcctcca acgtcaattc ctacacgata catcaaaatt tcctcctaaa cttttatttt
      481 ccttgtaatt cttctactgt tttatattcc acatcatatc caaagtatcc aggtccaact
      541 gtctcaattc ctttttctgt tctccatttt tcattacaag gaaaagctac taccataaca
      601 cgagagccat attttattct ctctgtagtt attggaaatc cagtttctaa atctaatact
      661 gctattaaat ctggtgtaat acaaagtggt gtatcacctt ttttggcaat taaattctca
      721 ttttggaata atatagaata ctctccatca tactcattaa tcccagcaaa aaaggcatgt
      781 cctctagtaa atccaccttt caactctctc ttaatatcaa ttacttttcc ttcaaataac
      841 ttgtatccac gcaattcttt tagtatattc tcaacagcac cttttgaatc agatttattt
      901 tcaagtaaaa accctcctat agtttcagct attgtaagag tattacctat agcagctttt
      961 ttaacttgag ctccacttaa attatagtca ctaatagaag aacttcctcc catttctaca
     1021 gttaaagttc tagctaatct ttctgaccaa attccatcca caggataaag gatggcagaa
     1081 ttaccttttt catcagacat tacggcagga gatgcctcat atccttcaag ataaaaagtt
     1141 accatttgag cttcagggaa agctctaccc atagaatctg catctaagat aggaagtcct
     1201 tttttagcag caactgctat aggcataagt gagtttatcc ctcctatttc tataggaatt
     1261 ataacatcca cttttctatt taataatttc tccattattt ctaaaggggt agttaactca
     1321 acttctgaaa gtaatttttc tattgtaatt acaggagaac ccataccaga tactggaact
     1381 accaaagcat catcatcaag ttcatctaaa cttattactt ttacaggacc atacttttta
     1441 atggcttgtt ttgcaaccaa tttacctaca tatgggtcac ctccacctcc agttccaagc
     1501 actgctgcac caactgctat tttatctaca ctttcttctg ttaaaaatct catgttactt
     1561 tctcctcata tttctaattt attttatact ttataaacaa atttctttat actgctttgt
     1621 ctatttcttg cttatttttt gttgaacaaa gcacttttcc aataatagat tgtactacaa
     1681 accctgctat aaaaccatct aaaggtgcta tagtagtaag agttataaat ccatatgttg
     1741 aaaggtaagt tattaatata ccaattatcc aagacactat tgaacgttta actattggtt
     1801 tatataatac tcctttatca aagcctttaa gttcttgctt tactatgtaa tattctgctg
     1861 tatatacacc accaacaggt gcaattacaa ttccaagtaa atttaaaaat cctataaaac
     1921 cttcatatat tccaaataca gccaataatg ttgctataat accaaggact atagttaata
     1981 ctttctcagg agccttctta agtacaagag ctatacttaa tgatgctgaa tatacattac
     2041 ttgtatttgt agtccattgt gctaatgtaa gtactattat acctggtata gcaattccta
     2101 atgtaataaa aattctcata atatctgaag ttcccataca ttttactaat actatcgaaa
     2161 ctattatcat aaaagcattt ccaaattgta ttcccaaaaa gcttgaaaga gcacagtctc
     2221 ttctactttt agcccaacga cttatatcag gagatacaat tgcaccaact attattatac
     2281 tcattgacag agaaactcca cctgcaaatg tcatagtaga tttcatatta tcatcaactg
     2341 aaattccatt tgctctaaaa gctaagaata tagttagcat cataagaact aataaaaatg
     2401 gtactgaata cacactcaac ttctccattg ccttatatcc ataaattgca gtactcatca
     2461 tcaagagacc acctattagg cttaaaatct gcatacttac atctaaatta aatatatctt
     2521 ttattattat ctgtgcattt tgagcaaaga aacctacttg aactccaaac caacctaata
     2581 gagaaacccc tataactaga gaaaatattt ttgctccagc ttctccaaat acatactttg
     2641 ttatcataga tgtagataaa cttgtagatg agccaaccaa agagcatgta gtatacaaaa
     2701 ttgcagaaaa aaatgaacca acaattactg acaatattgc ttgtttcatt ggcatactct
     2761 gtgccaactc agcgcctaaa ataacagatg ataaatccac acctattgct actaaaactg
     2821 aaaacatagc tacccaacca cgtttttgat tctctggaat ctgttccaga gcatagttaa
     2881 ttttttcttc ttccattttc atccctccat ttaaatcgta aaactactga ccttagcata
     2941 ttttacaatt ttctaattta taaatttatt aactatcata tttttataat tttcatttaa
     3001 tatatagttt taaattttct gaaataagtt tataataaaa aacgcctctt ggaaaaatcc
     3061 aagagacgaa tataaatccg tgttaccact cttattagta aaaattaatt ttactcactc
     3121 attttcttaa ggcgaattaa accactattc taacataatt acaatagtat tctccaaagt
     3181 ccttttcaca taaattccaa tattagttct cagccaaatc taactctctg taaaattcct
     3241 ttatgctact ttcttcttct acgaatatat aatattgatt tgtagttaat ttatgattta
     3301 tatcataata tatatgcaat attttgtcaa cttaaaaagt caattacttg cattttttag
     3361 ttttatatgt aaattttc
//
LOCUS       DAMJWS010000169         3330 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMJWS010000169 DAMJWS010000000
VERSION     DAMJWS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.174"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            94..849
                     /locus_tag="PC399_003682"
     CDS             94..849
                     /locus_tag="PC399_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2594480.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            946..1983
                     /locus_tag="PC399_003683"
     CDS             946..1983
                     /locus_tag="PC399_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2594481.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            2792..>3330
                     /locus_tag="PC399_003684"
     CDS             2792..>3330
                     /locus_tag="PC399_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594482.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMIS"
BASE COUNT         1261 a          435 c          502 g         1132 t
ORIGIN      
        1 ctttcctagc tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta
       61 ccaaaaataa atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag
      121 agtttcccta atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata
      181 gaagatggtg aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta
      241 aatataatat ctgggcttat aaaagaatct tctggtcaaa ttactttaga tagtactact
      301 ataacaaatt tagctgagta taaaagaact caaatagtaa gtagagtttt tcaagaccca
      361 agccttggaa cttgtccttc tatgacggtt agagaaaatt tatctctagc acttaataaa
      421 gggaaattat tgaacttaaa aaaatgtctt cgtcataaaa cgaatgactt ggaacactta
      481 cttgaaggaa tttctttgga tttaaaaaaa tatttagata ttaaagtaca atatctttca
      541 gggggtcaaa gacaatcact atcactaatt atgtctagct tagcaagtcc taaggtctta
      601 ttactagacg aacatacagc agcacttgac ccaaaaacat caaatgaagt aatagagtta
      661 acagataaaa tcgtaagaga gaaaaatata actacactta tggttactca caacttgaaa
      721 cacgctcttc aatatggaga taggttgata atgcttcata agggtgaagt tgtattagat
      781 gtaaaagata aagaaaaaaa agatttaaca gttgaagaaa tcttagaaaa atttgaatat
      841 gcagtataag aaattaaaat cttagtttta atcagaaata tttataatct atcatatgaa
      901 tatatgaaca aatataatta ttaaatttta agggaggaaa ataaaatgat aaaaagtaaa
      961 aaaatattaa gtttaattat agcaggagta cttggtgtat caatgcttac tggatgttct
     1021 caaaatgatg gctccaatgc ttcaaatgaa aataaagaaa cagatagcaa aaaacaaaaa
     1081 aatataggta ttactcaatt agtggaacat ccatctctgg ataaagcaaa gaaaggattc
     1141 atcaaagcac ttgaagataa aggctataaa gatggagata atataaaaat agatttccaa
     1201 aatgcacaaa atgatatgcc tactacacaa agtattgcta gtaagtttgt atccgacaaa
     1261 aaagatttga tatatgctat atctactcca tctgcacaag cagcttataa tgctactaaa
     1321 gatattccaa taataatgac tgctgttaca gaccctgtag aagctggact tgttaaatct
     1381 cttgaaaaac caggtggaaa tgtttctggt acatctgatt atctttcaat tgataaaaca
     1441 ttagaattgg ttaaaacatt gactccaaaa gcaaagaaaa taggggttat atacaatact
     1501 agtgaagtta attcaaaaat ccaagttgat tctctacatg attatgctaa gaaaaataat
     1561 tatgaagtag ttgaaaaagg aatcagctct tcaagtgaag ttaaccaagc tatttctagt
     1621 ttagttggta aaatagatgt tttatatgtc cctactgaca atttaatagt ttcttctatg
     1681 ccaatagttt ctaaagttgc taatgaaaac aagattccta taatagcctc tgaagaaggt
     1741 tctgtatctt ctggtgcttt agcttgttgt ggaatagatt atgaaaaact aggttataag
     1801 gctggagaac ttgctattga agtattagaa ggaaaatctg ttggcgatat accagttact
     1861 acattagatg aaactgaaat aataattaac gaagatacac taaaagcact tgacatgcaa
     1921 aagttatcag cagataatat aaagtatata aagtcagatg aaaatgcaaa atctacaaaa
     1981 taaaacttat taatttcaaa aaataggtaa tatgaagtag gtaatatgaa aactttatat
     2041 aataagtact cttatccacc ctaacccttt gaaaattgtg cttattgtag caatttgaat
     2101 attgcatata aagttattca ttttccataa aagtcgtcta tattgacgac tgattatgaa
     2161 agttatataa ctttctaatc actacttgag ctgtccaaaa gaattttaat attctccaga
     2221 cagctttttt ttatgatatt tgattgatat acatgtctag ttattccata tttagtaaat
     2281 attttgataa aatttccaca ttcacttata acttaggata acgttttttt caaacaatac
     2341 attaatttaa aagtattctg agtatacatt atttagtatt ttaattaaat ttagcagttg
     2401 acttttatta ttgtataatt tataatttat aacaagtcga gaatacatat aattttctga
     2461 ctaattaaaa tattaaaagg gagagataat tatgaatact ttattaaaca atagaaaata
     2521 ttattattat tatagccact atttttaggg tttgtaccta cgttttaaca tagatacaga
     2581 ccccttaaga ctactaaaat tttgggattg tatctatgtt ggctatattg tttataaagt
     2641 tttcatttaa ctatataaga gccacataga ttaaactatg tggcttattc gtgtttttac
     2701 cacatagttt tgtacactat gtggtttttt tatacacaaa attagcaaat aattaaaatt
     2761 ataaaaatat tttcaaagga gtgttttatt tatgtcaggt attatatctg taatgacgca
     2821 aagtttaatt ttatcgatta tggcactggg ggtttatata acttacaaaa tcttagattt
     2881 tccagatatg tctgctgatg gtagctacac aatgggtgct tctattgtag cattttctct
     2941 tactaatggt atttctcctg tagtagcaac tttaatggct atcttatgtg gttgtactgc
     3001 tggtcttgtt acagggattc ttcacattaa gtttaagata tctaacttac tttctggtat
     3061 tttagttatg ggaatgctat actctatcaa tttaagaata atgggaaaat caaatattcc
     3121 tctatttagc tttaaacact tattcaatgg ggaaatatct cctatagttt tagccttagc
     3181 ttttgtattc atctgtaaag tccttttaga tttattccta aaaacaggtc ttggatacac
     3241 tttaaaaggt gttggtgata actctcaaat gattaaatcc ctaggtataa atatcggttc
     3301 tataaaaata ctaggactta tgatttcaaa
//
LOCUS       DAMJWS010000170         3310 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMJWS010000170 DAMJWS010000000
VERSION     DAMJWS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.175"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            251..1519
                     /gene="eam"
                     /locus_tag="PC399_003685"
     CDS             251..1519
                     /gene="eam"
                     /locus_tag="PC399_003685"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF2594483.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(1612..1968)
                     /locus_tag="PC399_003686"
     CDS             complement(1612..1968)
                     /locus_tag="PC399_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594484.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(2119..3213)
                     /locus_tag="PC399_003687"
     CDS             complement(2119..3213)
                     /locus_tag="PC399_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2594485.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         1353 a          436 c          471 g         1050 t
ORIGIN      
        1 taattcttaa aaatagaaaa aattaatcat ttgttaaaaa aaatgcataa tttattgtaa
       61 tttattgacg aaggttgaaa aatattcata aatgtgcgaa ataacagaaa aaatgcaaaa
      121 attaattcaa tttcatttgt tataaaatct atatggcaaa attaaatctg cctatcagaa
      181 tgatataaac acatcagaaa ggaggacgct atcaaataat acaagtagtt aactttgata
      241 gcaaaaaatt atgaatgaac aaactagaat atccttagag agagctgctg aattaaaatc
      301 aaaaattgat gattatattc aggctagaaa aacgattaac agaggtcttg aaaaagaaga
      361 agagataaat aaacgaaaac agaaaatatt aagtatctta aatggaactg aagaggattg
      421 gaataactac aaatggcaat tatcaaatag aataacagat gtagatactt tatcaaaaat
      481 tataactcta actaaaaaag aaaaagaata tataaaagag gttggtactc aatttagatg
      541 ggcaatatct ccatattatt tgagtcttat agacccagaa gatatatgtg acccaataaa
      601 attactgtct ataccaacac atatagagtt ggaagatgag caagaagatt tggacccaat
      661 gggagaagag tatacaaacc cagcaggatg tataactaga agatacccgg atagattaat
      721 aataaatgta acaaatgagt gtgctatgta ttgtagacac tgtcagagaa gaagaaatat
      781 tggacaacaa gattctcata agtcaaaagc tattatccaa gaatctatag actatatcag
      841 agaaaatgaa gaaataagag atgtactagt aactggtgga gatgctctta cattaaaaga
      901 tgattattta gagtggattc ttagccaact taaagagata ccacatgttg attatgttag
      961 attaggtact aggactcttg ttacaatgcc acaaagaatt acagatgaat tttgcaatat
     1021 gctaaaaaaa tatcaccctg tatatataaa tactcatttt aatcatccaa tggaaataac
     1081 taaggaatct aaagaagctt gtgaaaagtt agcaaatgca ggcgttccat taggaaatca
     1141 ggcagtatta ttaaatggaa taaataatga taaatttgta atgagatgtt taaatcaaga
     1201 attactgaaa ataagagtaa aaccttatta tatattccaa agtaaacatg taaagggaac
     1261 aaaacatttc aatacatcag tagatgatgg tcttgaaatc atggagtatt taagaggata
     1321 tacatcagga atggctatac caacatatat agtaaatgct ccaaaaggag gaggaaagac
     1381 tcctttgctt cctcaatacc ttgtatcaaa aggaacagat tacgttatgc ttagaacatg
     1441 ggaaggaaaa gttataaaaa tggaagatga acctgctgta gatataaaga aacttataaa
     1501 agaacaagca caggattaaa aaaatattta aagtttataa aaagaaacaa ctttaaaata
     1561 aaaagaaaat ggatgtcaaa tttatatcaa tttgcatcca ttttttatta gttatttatt
     1621 attttgttgt tgattaacta attcaagata tccacttggg tggaagtaat atatctcaga
     1681 agtattttca tttacaaagt aaccaatatc accatctgca tctggtaaaa atacataacc
     1741 attaagattt ttagctttta atgcttcaaa atcactagca tttccttgat atatgtaatc
     1801 aacttcattg ttgtatttta ctaaaagttc tttagcttgt tcttgagtca atttagtatt
     1861 ttggcttaca ggaacattct tttgagaatc tgcataagaa gattcattta ctggtgcagt
     1921 taataacaaa caagataaaa ctaataaaga tataagtgct tttttcatac taaaaccctc
     1981 tttcatgttt aattatataa gattcattac ttaataatat tgtatgtttc ctataattaa
     2041 atattaccat taaagagaat aatattacag ttacacagct gtaacttttg attacaaaat
     2101 cgtaattaaa ttgtattttt attccatttg tattttatca tgtctacgat ttacaaactc
     2161 acagaagtat atctttataa cgcccatcac aggagcagat agaatcatac caggtatacc
     2221 aaagaatcct ccaccaacag taactgctag gattgtaaag aatgggctaa gacctagttg
     2281 tccacctacg atttttggct ctataaatgt aacttcaact tgttgaatta ttattaagta
     2341 aattaaagaa gttaaagcaa ttgttggatt ggagaataaa ttaattatta caactggtgc
     2401 catacctatt actggaccaa aatatggaac catattcatg acacccataa gtgtaccaaa
     2461 taatagtgca tattcagatt ttaaaaagta aagaccaatt ccagaaagta ctccaactat
     2521 aaaagaatct aaaatcttac ctgtaaagta tttaccaata tttaaattta agctttgaca
     2581 tagttctatt ataaagtctc catatttttt acctaaggaa atataaacta ctttttttgt
     2641 aaacagtaag aatttttctt tttctaataa tatatagaaa caaattataa aacctaaaag
     2701 aaattgtact ataaattttc caactgaaaa tgtagtgcta aatatcccac caagtgaacc
     2761 aataagcaag ttactaaatt taggcattac actcataatt ttgtcaccat actcttttaa
     2821 tgtattaggg tcaactgatt gtaaactctt gcctaagtcg aataaaaact gctctgtttt
     2881 atctatgtat gtaggaattt gagcaactaa atcagctaaa ctatttacta tggatggaac
     2941 tgtaaataat ataaacgaaa ttagtataac tatgagtaca ccataagtaa gaagtaacga
     3001 atatattctc ttaaaattta atttactttc taagaacttt actagtgggt tgaatatata
     3061 tgctaataca aatgctatta taaatggtgt tagtagtgac attaataaac ttatcactcc
     3121 aaaaaaatat ttgtaattat caataacttt aatcaagata tatgaaacaa ctacagtgag
     3181 tacaatattt agataaattt ttttatcttg caaaataaca caacctttct tatatttatt
     3241 aaatttagat atacttaaat ctaagttaaa gttaatattt atagcttatt atacaataaa
     3301 aagcagtatt
//
LOCUS       DAMJWS010000171         3198 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMJWS010000171 DAMJWS010000000
VERSION     DAMJWS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.177"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     repeat_region   117..1266
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=183..211
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(1462..1701)
                     /locus_tag="PC399_003688"
     CDS             complement(1462..1701)
                     /locus_tag="PC399_003688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594486.1"
                     /translation="MECYLKRTLIIFSEAFLSISFKTRKYLFIVIFILVCPNLSEMIF
                     ILVPARRRIIRIDNIVSIYKYVGVLKLINYSILIL"
     gene            1913..3184
                     /locus_tag="PC399_003689"
     CDS             1913..3184
                     /locus_tag="PC399_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2594487.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1269 a          427 c          408 g         1094 t
ORIGIN      
        1 aaactaaagt caatgttatc tggtaattat ttactacaaa tgctaaaaaa taattatatt
       61 ttaaaggata aaatgctgta taaattattg gggtatttta cctatacttg ttgcaaattt
      121 acataccacg tagttaatat aaaacaagat gtttttaaaa ataaacttaa tatagatatt
      181 gaatttacat accacatagt taatataaaa ctttaatcat atatcagata ggtcacaact
      241 taagagtgat ttacatacca catagttaat ataaaacaac ttctcatatt ccagataatt
      301 gttattttac gaaatttaca taccacatag ttaatataaa acctgtagat ttgcctgttt
      361 cgatattgct catgtgtgaa tttacatacc acatagttaa tataaaactc tcttgtttct
      421 tctgttaatt ttattatacc ttcattattt acataccaca tagttaatat aaaacgcgtt
      481 atattgttgt ataaaaccga ttaaagttca atttacatac cacatagtta atataaaact
      541 tgcaacatat tgttttatcg ttgctgggtc tttaaaacca tttacatacc acatagttaa
      601 tataaaacta agcgatatta gagagcgcaa ggcatagcgg agcaaattta cataccacat
      661 agttaatata aaactttaat tttaaccacc ccctttcact aataaaaaaa gatttacata
      721 ccacatagtt aatataaaac aagacgctat acaagaggta atttgaaagc aatctaattt
      781 acataccaca tagttaatat aaaacgttgc atatgtatgt ctaagagcat gaaatttaat
      841 tttatttaca taccacatag ttaatataaa acttctatta agtgtaagca aaacgcttgg
      901 aatatgaaat ttacatacca catagttaat ataaaactta tacacaaata caacttgaag
      961 aagcgctaaa gagatttaca taccacatag ttaatataaa acctaatgag tacatagata
     1021 aacgtacagg agaggttaat ttacatacca catagttaat ataaaacgtt gataattttc
     1081 gtttgtatac tttttataat ttaaaattta cataccacat agttaatata aaacataaag
     1141 atttagaaaa agaaaaagtt aaaattttgg aatttacata ccacatagtt aatataaaac
     1201 ttttcctgtt aagttgatgt ttgcaacttt aactgctatt tacataccac atagttaata
     1261 taaaacccca aaataaacta agtattttca gtacttacac acatacaact ctctcaaatt
     1321 tgcagtgaac catgagtagt gcaattgata acatttatca cacaccctca atcccttaca
     1381 ttgcaactgt taaaccctac tttatcaaaa atatcgctca ctgcaaaatt tatatatttt
     1441 tattatatca taaaattcaa actataaaat taaaatggaa taatttatca atttaagaac
     1501 acctacatat ttgtaaatac ttactatatt atctattcta ataattcttc ttctagctgg
     1561 aactaatata aaaatcatct ctgaaagatt aggacatact aatataaaaa taactatgaa
     1621 tagatatttt cttgttttaa acgatatgga taaaaaagct tctgaaaata taatcaaagt
     1681 tctctttaaa taacattcca ttttatgtaa gtaggcatta tatttttctt aaatgtcact
     1741 taaatgtcag ttatatataa taatagtaaa caactgtatt tcactaatat ttgctattat
     1801 ctaatagtat ttatagttta gatttgatat aatacatgtg gtaaaatttt gatttgtaaa
     1861 acaaaatttt acctacatat attaaaacaa atttaaaact aaggagacct atatgttaaa
     1921 agtattaaaa ggaaatataa tttttacaaa aacttcagaa actttcacag tatttgaaaa
     1981 tagttatatt atactggaaa aaggtaaagt aaagggaata tttaaagaga tacctaagga
     2041 atacaaaaac ctaaaagtag tagattacac tgataagctt ataatacctg gtatgaatga
     2101 cctccatgcc catgcctctc aatttaaaaa tcttggaatg gctatggata aagagctttt
     2161 gccatggctt gaaacttata catttccaga agaatcaaat tataaagata tagaatatgc
     2221 aacaaaaatg tataaaaaat ttgtaaaaga actttggaaa tctggaacta caagaatagc
     2281 agtgtttgca acagtacata aagaagcctc tatgaaactt atggatatat tcaaagaagt
     2341 tggtcttgga gcatacgttg gtaaagtaaa tatgaatatg aattgccctg atactttatt
     2401 agaaaacact caggattcta ttactgatac agaagaaatt ataaataagt atagtgatac
     2461 agattctatt gtaaaaccaa taataacacc tagatttata ccaagctgta caagtgaact
     2521 tttaaaaggt ttaggagatt tatgtttaaa atataatatt cctattcaat cacacttatc
     2581 tgaaaattat gatgagattg aatgggttag aagtctagaa cctgaatcta aattctatgg
     2641 agatgcatat aatagatata atttatttgg tcaaactcct actttaatgg ctcattgtgt
     2701 tcattgttct caggatgaaa tagatttaat gagagaaaat aatgttatgg cagttcactg
     2761 tccagcttct aactttaatg ttggtagtgg tgctatgcct atcagaaaat taatcgataa
     2821 taatattaga ttagcacttg gatcagatat aagcggtgga catacattgt caatatttaa
     2881 agcaatggta tctgcaatac aattatctaa attatattgg gttaattctg gtaagaagta
     2941 taatttctta tctttatctg aggcttttta tattgcaacc aaaagtggtg gaagtttctt
     3001 tggaaaagtt ggtagctttg aagaaggtta tgattttgat gcattgatta ttgatgattc
     3061 aaatttaaat catgacaatt attctatact tgaaagactt gaaagattta tctatgttgg
     3121 tgatgataga aatatcattc atagatatgt atgtggaaaa ctaattgaag aacctaatat
     3181 ataattataa taaaaatt
//
LOCUS       DAMJWS010000172         3170 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMJWS010000172 DAMJWS010000000
VERSION     DAMJWS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.178"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            273..887
                     /locus_tag="PC399_003690"
     CDS             273..887
                     /locus_tag="PC399_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2594488.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTNRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            905..1267
                     /locus_tag="PC399_003691"
     CDS             905..1267
                     /locus_tag="PC399_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594489.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1373..1870
                     /locus_tag="PC399_003692"
     CDS             1373..1870
                     /locus_tag="PC399_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2594490.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            2674..>3170
                     /locus_tag="PC399_003693"
     CDS             2674..>3170
                     /locus_tag="PC399_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2594491.1"
                     /translation="MIAVKKAYKFRIYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKGRILNATISKEPSGR
                     YYVSL"
BASE COUNT         1259 a          358 c          516 g         1037 t
ORIGIN      
        1 ctatataata atttattcta ttagtatact caaatcatgt aaaatttata taactcaatt
       61 aattttttaa atacaacttt ataatataaa acttataata aaagtaaatt tatatgttaa
      121 atgaggttga ttttttatta ttttttattt atgctagtac taaagacttg caaacgtttt
      181 ttgacaagtc tttattttgt ctaaattaaa caaacttttt tacaaataaa tacataaaat
      241 ttaaactaat ctatttattg gaggagataa aattggaaga agtaattgca aaaattatta
      301 ctatagaaaa tggattcaaa gaaattgaaa acattgctaa aaaaattgta aaaaataatg
      361 actcaaaaaa gtgttattat ctatcaatct acttatatag gtcagaatat tatcaagtaa
      421 gagaattggc tgtgtttata cttggtttta tatcagtaac tatttctgaa gctttgttat
      481 ttttaaaaga taatgtgagt aaagatgaaa actggagagt acaagaaata ctagctaaat
      541 catttgatta ttattgcagt gaagtaggat atgaaaaggc acttcctgtt ataaaagaat
      601 ggcttaattc tgatagtcca aacataaaaa gggctgtgac agaaggtctt cgaatttgga
      661 ctaatagaga ctattttaaa tctaatcctg acgttgctat atcactactt tctagtttaa
      721 aagaagatga tagcgaatac ttgagaaagt ctgtaggtaa tgctcttaga gatataagta
      781 aaaaatatcc agatttagta aaaaaagaat tagattcatg ggatatatca aataaaaaag
      841 ttgagtttgt atataagctt gcttcaaaat gtatcttggc aaagtaaaat ggaggtgaac
      901 ttatttgaaa cttcgtgatg agtatacttg cccattagaa ctaacacatg atatgataaa
      961 aggcaaatgg aaatctatta tattatggag attacgcata ggaccaactt ccttatcaaa
     1021 acttgaaaag gaaatcaatg gtattaatca aaagatgtta cttgaacaac ttaaagaatt
     1081 aattgaattt ggtcttgtag ataaaaagac atatgaaggt tacccactta aagtcgaata
     1141 ctcacttaca cctattcgag ggaaacaaat attggatgct ttggctataa tgcaagatat
     1201 aggaattagc tttatgttgg ataatggtat ggaagatatt cttaaggaaa aaggtgtttt
     1261 aaaataaata gatacccaca aaaaagtaag taatttacca ttattttcaa ctggtatata
     1321 attaaattat agatatattc tattaattta ttataatagg aggtaaatta ttatgaaagt
     1381 aactagtaca ggtatagtaa atggtgttat agaagataaa tatggtaaaa gaggaactca
     1441 gttcaatgaa ggtggtatgc ctacatattc actacctcta aagtttgaag atgctccaaa
     1501 gaatacagta tcctttgctg tatttttaga agataaagac tctgttcctg tatgtgggtt
     1561 tgcatggata cattggttag ctgtaaatat aacaaaagat gagttaaatg aaaatgaaag
     1621 tgtaacagct actgattttg tacaagggac tactagttgg catggtaaac taggaggtct
     1681 tgacagatta gaggccagtg tatatggtgg aatggctcct ccagacagac ctcatgtcta
     1741 tgaaatacat gtatttgcac ttgatactat ccttgacctt gaaaaaggat tctatatgaa
     1801 tgaatttttc aaagctattg aagggcatgt tttagataca tttacactaa aaggaagtta
     1861 ttctaattaa ttaggttgac tatagctaat cattaattaa atgatacatt taattgatga
     1921 ttagcttaat tgttattaga atattttctg cactttatct taatatatat tttagttgac
     1981 tggtatttta atttctatta gattattatc aatttatata aattgattaa aaaatagcta
     2041 cgcacactcg tgactttagt catgagttag tagctaaaat agtcagcata tagggaaact
     2101 tatatgtaga gataggatag aacctatcca atactacttg aattgctaaa aacccctaaa
     2161 gctaaccaaa ctaaaacata aggatgaaat aaacctaagt gtgaaagtta cgaaagtaga
     2221 aaaaattggt tagatggtat aaggttaaac cctaagtact ttaaaatggg caatcagcag
     2281 gtaaactcca aataggagaa acttcaacga ctattcctct tgagggaagt acactacaag
     2341 ttatcggtag tggaagtggg tagaccttag cagataatgc cgaaggataa gatatagtct
     2401 gtgcttatgt gaaagtgtaa gaagttcata agagaactgc ataggtagta gcgaacttat
     2461 gtgaacgaca acctcaaaaa tgataaaagt taagacttac ggttcttata tatgttattt
     2521 aaaattaaat ataaaacctt ctaaataatt gttttaaata attacatttg ttaacattat
     2581 tataggtgat taataaattg ttcaccacag cattatataa tatattaaat attaaaatca
     2641 ttaaaaagtg tatcaaaaaa caattaaagt gaggtgatag cagtgaaaaa agcttataag
     2701 tttagaatat atcctaataa aaaacagcaa gagttaatta acaaaacttt tggatgttgt
     2761 aggtttgtat ataataaata tcttgctaaa agaatagatg tttataaaaa cgataaagaa
     2821 acttttactt ataaacagtg tagttctgat ttaaccaatt taaaaaaaga attaaattgg
     2881 cttaaagaac ctgataaatt ttcactacaa aacgccttaa aagatttaga taatgcatat
     2941 aaaaaattct ttaaagaaaa ggcaggattt cctaaattta aatcaaagaa aattaataga
     3001 ttttcatata aaactaactt tacaaatgga aacattatgt attgtggtca acatataaaa
     3061 ctacctaaac ttggtatggt taaggtaagg gataaacaag tacctaaagg gagaatactt
     3121 aatgctacta tatctaaaga accaagtggt agatattatg tatcattatg
//
LOCUS       DAMJWS010000173         3164 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMJWS010000173 DAMJWS010000000
VERSION     DAMJWS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.179"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            105..2462
                     /locus_tag="PC399_003694"
     CDS             105..2462
                     /locus_tag="PC399_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF2594492.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2548..2865)
                     /locus_tag="PC399_003695"
     CDS             complement(2548..2865)
                     /locus_tag="PC399_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF2594493.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1229 a          337 c          506 g         1092 t
ORIGIN      
        1 ttttattata taaactatag aataataaaa taaaaaaatt gagaatttta taatatattt
       61 gtagatttga gtataaaaaa gtgctaagtt gaaaggagta aaatatggat aaggttaatt
      121 tagaaaggta tagttgtgat ataaatgttg gtttaaataa aaatcaactt caaagtagaa
      181 tagatgataa tttaattaat gcatttgata atggaaaaac caaaacatat aaacaaattt
      241 ttaaagacaa tatatttaca ctttttaatc tcataaatat tgttttagtc tgtttattag
      301 tttcagttgg ttcttttaaa aataccttat ttatatttat tgctgtaatt aatactatta
      361 taggagtaat acaagagata agagctaaaa gattattgga caatctatct gttatagttt
      421 caaataaggt atctgtcata agagaaaaag agaaaaaaga gattgatgta caagaattag
      481 tattagatga tattatgatt ttaaaaacag gtaatcaaat ttgtgcagat tcaaaagttt
      541 taaatggaaa attagaggta aatgagtctt tggttacagg tgaatctgat attattataa
      601 aaaataaagg cgatttttta tattctggaa gttttgtagt ttctggagaa gcttttgtaa
      661 gagttgaaaa tataggaaaa gataactatg caaataagat agctaatgat gcaaagattt
      721 ctaaaaaaca taattctcaa cttagaaatt cattaaatgc aatattaaaa attgtaggta
      781 taataataat tcctttagga attatattgt ttgcaaaaca acattttgga aatggagatt
      841 ctatagctac atctgtagta ggtacagtgg cagctgtaaa tggtatgata ccagagggat
      901 taacattact tacgagcatt gctctagctg taggaactat aaaacttgct agtcataaaa
      961 ctttagtgca agaactgtac tgtgtagaga ctcttgctag agttgataca ctatgtcttg
     1021 ataagacagg tacaattaca gaaggtaaaa tgcaagttga tgatatagta atgttggaag
     1081 aagtggatat taatgaaata atggggaata tatgtaattc tctcaaagat gataatgcaa
     1141 cactaaatgc aataagagat aagtataatg ttttagatac gtataaagct aagaatttaa
     1201 taccattttc ttcagaaaga aaatacagtg gggttacctt tgaaaataaa ggtacatatt
     1261 tattaggtgc atttgagttt atatttaagg aaaaaaataa aaaacttagg atagaagttg
     1321 aaagatattc taaaaaagga aacagagtaa tagttttagc tcatagtgat tcttatatgg
     1381 aagataataa tattcctgaa gatataaagc cactagcttt tatattgata ttggataaaa
     1441 ttagagatga agctaaggaa actttagaat ttttttacaa tgaaggtgta gatataaaaa
     1501 ttatatctgg tgacaatcct ataacagtaa gtgaggtagc tagaaaagca ggtcttaaaa
     1561 cagctagtaa ttttattgat gcaacaacac ttaaaaatga tagtgatata tacaaagcag
     1621 ttgataaata cagtgttttt ggaagggtaa gcccacaaca gaaaaaacaa atgattttag
     1681 cattaaaaaa acaaaatcat acagtagcta tgactggaga tggtgtaaat gatgtactag
     1741 cacttaaaga agcggattgt agcattgcca tggcatctgg aagtgatgtt actaagaatg
     1801 tgtctaattt agtcttatta gattcaaact ttgcatcaat gccaaaggtt gttatggagg
     1861 gaagaaaagt aattaataac attcaaagag catcttcttt gtttttagta aagacaattt
     1921 tttcattctt cttgtcgtta ttaacattat tcttcttcag tagacagtat ccatttgttc
     1981 ctatacaatt atcactcatt ggggctgtaa cagtgggtat accatcattc ttcttagctc
     2041 ttgaagcaaa caaaagtaga atttcaggaa attttttact caatatatta aaaaatgctt
     2101 taccaggagc actatgtgtg acagtaaatg taattattgt ttacaatata gttgaatatt
     2161 taggttatag ttcaagtgta ttttccacaa tgtgtgctat attaactggt gtatgtggtc
     2221 ttttaatatt gagacaacaa tgtttgccat ttaataaaga aagattattt ttaatagtaa
     2281 gtatgactat tgcatttgtt attgggattc tatttttagg tggattattc tcatttgtag
     2341 cattgagtag agaattaatt ctcataacta tggcattagc aatattaatg ccaattatga
     2401 taaaagttat gttatttgtc ttagatgaga tattagagac aattgttcca gatttaaatt
     2461 aattttaaaa tgatataaat aagagcagtt ttagttgaac tgctcttttt agatgtgttt
     2521 aattttatgt aagaatatgt atttttatta atcagctata tatgagcaac agtagtcaac
     2581 aacttcatta acctttaagt caaaacttga atcactagga acattaaatt tttgcccttc
     2641 tttataagtt acaaactcat tgcttccagg cagttttaca tccatacttc cagcaagcat
     2701 ctccattatt tcttcttctc tagtagaaaa agtgtattct cctggcaaca ttaatcctaa
     2761 agtttttctt tctccattag gcagtattat tactctgcta cttaccttac catcaaaata
     2821 aacgtttgcc ttctttactg cagtaacatt cttaaattca ctcattagat aaaacctcca
     2881 aaattatttg tacatatata ttatataaaa ataatgtaaa aaatgctata tatttacaaa
     2941 aaaatttagt atttattaaa aaaattaata agaataaaaa agaggtgaaa ttgcttaaat
     3001 tgataaaaaa tataaaataa attgcaaagt aagcaagaaa agaataagtt gaagagttat
     3061 atattataaa tattaaaaat tttagtatta ataaaaaaga tattacatta atatgaaaat
     3121 ataggagatt agaagtgttt gattaaaaaa tagctacaca cact
//
LOCUS       DAMJWS010000174         3088 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMJWS010000174 DAMJWS010000000
VERSION     DAMJWS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.180"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            172..966
                     /locus_tag="PC399_003696"
     CDS             172..966
                     /locus_tag="PC399_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2594494.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            967..3027
                     /locus_tag="PC399_003697"
     CDS             967..3027
                     /locus_tag="PC399_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2594495.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1188 a          319 c          507 g         1074 t
ORIGIN      
        1 aaaaaatgtt acaaaagata ttataaacga ataaaaaata ataatttgcc agataaactt
       61 gtaaattttt atgaataatc cattctaaaa caaaaaactg tgtataaaaa aggtttatat
      121 gttatattta tataaatata acatatgaac gagaaaggag tggtttgata aatgattgag
      181 gtagataaat tgtgtaagtc ttttacaagg gttgtaaaag atgataagaa taaaagttca
      241 ataagaaagc taaagaaagt aaaaactaaa aaagaagaat tttttgctgt aaataatgtt
      301 tcatttaaag ttatggaagg tgaaattgtt ggaatattag gtccaaatgg tgcaggaaag
      361 actacattac ttagaatgtt aggtggcata ttaacaccaa catcaggaag tataaatatt
      421 tctggatatg attattcaat agatagaaat tcagcaaaaa aagaaatagg ttatctctct
      481 ggaaatacaa aactatatgg aaggctttct ccgagagaac ttctaacaac ttttgcaagt
      541 ctatatgaga tgtcaaaaga agatatagaa gaatcaattg aaaatattgt aaaaataatg
      601 gatatgagtg aatttataga taatagaatt gaaaatctat caactggtca gactcaaaga
      661 acatctatag ctcgttgttt aatacattct ccaaaagtat atatttttga tgagccaaca
      721 ctaggtctgg atgttttaag tagtgcatct attatagatt ttatgaagaa tgaaaaacta
      781 aggggaaaaa ctgttcttta ctcaactcat tatatggaag aagcagaaac tttatgtgac
      841 aagattttta tgattcacaa tgggaaaata attgcatcag gaacaccaca gagtttaaaa
      901 gaggaatctg gagtaaataa tttgagagat gtattcataa aacttgccag aaaggaggac
      961 atttaaatga gaagtaagat tgtaaaatac atatttaaaa aagaaatgtt agacatattg
     1021 agagataaaa agactttatt tatgatgatt gttgtgccac ttcttttata tcctttactt
     1081 atgttattaa tgattcaagt aatgaatatg tctgcaagta gtatgaatag taaggacatt
     1141 aatatagctt ttaataataa acctaataag gttttagtat ccatgattga agatgataat
     1201 ttagaaaaag atactaaaaa tacttcaaat gataaaggca aaataagagt gttagatgta
     1261 gataactata aaaagtcatt agaagaaaat aaaattgatg cttatataaa gatggaagaa
     1321 aaaaaatcat ctataaatta tcaaatatat ataaattctt ctcaagaaaa ttcttctaat
     1381 gcactaaaaa gaattgaaac tgttttggat aaatataaaa gatttaccat agaaaaaact
     1441 ttatctgaaa aaggattaga tgtacaagct actttagaac caataacata tagcactgta
     1501 gatgttgcaa agacggaaga agtggcagga tactttttag gtcagatact tccatttata
     1561 ttaataatag gagttttact aggttcaata tatccagcaa tagatgttat ggctggagaa
     1621 aaggaaagag gtacattaga aacattattt acactgccaa tatcaaactt agaactggtt
     1681 atgggaaaat atatggcagt ttctcttagt gcaatagtaa cagcaatttt aaacatttta
     1741 tctatgggac tgactatggc atttatttta gttagtggag gtattagtag ccaatttggc
     1801 tttggaaact ttaattatgg agacttagtc ttaccaatta taactacttt aatttgcata
     1861 tgtctttttt caatggtagt ttcagcaata agtatgtgtg tatgttcatt agcaaaaagt
     1921 tttaaagatg ctcaaaacta cataacacct cttatgttaa ttattatgat accatcatat
     1981 gtttctatga taccaaatat agtattagat agagtgacag ctgtaatacc tgtagtaaat
     2041 atatctttac ttataaaaag tgtattatct tttaaaagcg atttaaattt aatagcctta
     2101 gttttagcat caaatgtagc ctttgttata ttggcaatca tactactgtc taagatgttc
     2161 aattctgagg aaatactttt tggtaatagt aagagtttct ctttcttaga gaaaagaagt
     2221 aatataaaaa aagggtctat accaactgta agtgatggtg ttattttata tgcagtagga
     2281 ttagttctat tgatttatgt tggttcttta gttcaaatta aatttggtat ggcaggtatt
     2341 ataatgacac aattcatgat aatttcttta ccgttattat ttgcatacta tattaaagct
     2401 gattttaaag aggtatttag cttgaaagtt cccaaattaa agcatgtgtt tgggagcatt
     2461 gttctttgga ttggtggata catacttatt atgattataa cacagctaat tttattctta
     2521 tttcctcaaa atatggaagt tgctgaaagt ttaaataagg ctctttttat caaagatagt
     2581 gttttactga atttactagc aatagcttta ttaccagctt tatgtgaaga aatgtttttt
     2641 aggggattta tattttcttc atttagtaaa tcaaaagata aaaataagtc tatcaagcta
     2701 gctataatat gcagtggggt actatttggt attatgcata tggattttat tagaataatt
     2761 ccaacatcaa tattaggtat tatatttgca tatagtgtgt ataaatctgg ttcaatattt
     2821 gtatcaatgt tattgcattt tttaaataat agtgtagcag ttgtgttaaa tcattataca
     2881 actggaaata taaccaactt atataaattt gttgaagttg atttttcgaa cttaaatgta
     2941 cttaaatttg ccttaattct tctaataagt cttatattaa ttatgttagg attaagagtt
     3001 ttagatagaa agtctttaag aaattaagta taaagttatt atatagatta aaatattaat
     3061 aagcactaaa gtatatggat atatcttg
//
LOCUS       DAMJWS010000175         3008 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMJWS010000175 DAMJWS010000000
VERSION     DAMJWS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.181"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            163..1056
                     /locus_tag="PC399_003698"
     CDS             163..1056
                     /locus_tag="PC399_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2594496.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            1493..1984
                     /locus_tag="PC399_003699"
     CDS             1493..1984
                     /locus_tag="PC399_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594497.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            2012..2959
                     /locus_tag="PC399_003700"
     CDS             2012..2959
                     /locus_tag="PC399_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594498.1"
                     /translation="MRITKTTFNDVFSSDANESIIKVEKYVDKKCASVFEILTYTIIV
                     TNTNRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         1315 a          265 c          424 g         1004 t
ORIGIN      
        1 aactattttg tgtgatagaa aactatatat tataacaata gtagtgcttt taattaaata
       61 tatttcataa atcttcataa aatcttcaaa atttcgtaaa gaatatgtca caaaagtaag
      121 gtatattata ttcagcagat agtataaggg aggacaatat ttatgaagaa taaaaaattc
      181 attgttataa tactgataat atcaatttta ataggtgtat tagtgaagga atattcatct
      241 agagagataa aaaaggatga tataaatgtc agtaagtata taaaatatgc tgatttagca
      301 agtaaaaata atgcacaagt taactggaag tatgtagctt ccattgttgc tgtgttaaat
      361 aaaaataact taaaaaacgt taaggattca cagatacaag aagtatcaga tttatttgtt
      421 aagaattttt ctaaaaataa taaaataaat aagttaagtg atattttgga tgagttagaa
      481 tttagtaata gacaaaaaag attggttgat aattatatag acaatcttaa ggattatggt
      541 ataaaaccag aaagattgaa gtctgataca aaatatatga agtttattga tgaaattaaa
      601 acagaagcaa tacaaaatta taaggattat aaaatacttc catctataac tattgcacaa
      661 gctataatag aatctagctg gggcaagtca actttggcaa aacaatatag caatctattt
      721 ggtataaagg ctgacgctta ttggaaaggt aagtcagtga ctttagaaac gaaagaacat
      781 ttagatacaa ttatagatga taaatttaga atatatgatg ataaaaatga atctataaaa
      841 gaccatgcaa aatttttagc cacaaataaa aggtataaaa acaatggtgt atttgatgca
      901 aaaacgtata tatatcaagc taaagcatta gaaaaggcag gttatagtac tgcgaaggat
      961 gaaaatggca atagtatata tgcagctcgt ctaatagaat taattcaaca gtataatctt
     1021 caattaatag acagtgaaat tcaaagtgaa atgtaacttt gattaaattt ttattgataa
     1081 ggctatgatt tttaaaatca tagccttatt ttgacatgct tttacttgta attattaaat
     1141 cgatatcatt atctttcaaa atttacttta tactgtaaat caaattattt aagttaaaac
     1201 ttggaattat ttataaaagg attatttact ttatctaaat tctgcactta aagaacatct
     1261 tttcccttaa gactgaccgt tagtctataa aaattataat tacttataaa aaagtgaata
     1321 tatatataaa gttttcagaa agacttttac ttgtaaattg aaatcaatga agtaaaaaag
     1381 gattctttct taaaagtaaa taacaactag atagtgaata ataaaaaatg gtaatttgaa
     1441 ataattttta tgaatagtat taatatatag atacagggag attgatgcga ttatgtatga
     1501 aaataataag gtaatataca accttgaaag atgtgaaaaa ctagaaaaga ctctttattt
     1561 atctcaattt aatacatata aaaattttaa attagcttct aaaaaaactg taataaattc
     1621 attagagaca tatgtttaca atatgaatat taacaatata agtcttgttg atactataga
     1681 atgtatatca ttagaagatg aacaacttac aggaaagaag ttgattttag ttggtgatat
     1741 agatattgat gcaatattag attatgcagg aaataaaaga aatagaaatt ctagtaaaaa
     1801 aaatacattt aaattaaaaa taccctttag tacatttata caaatgccta gaaagataga
     1861 aaataaagat aaaattaatt taaagtatct tatacaagat attacttcat ctattttaga
     1921 tgataattta tttattagtg ttactgcaat tataagttat gaaaatagtt ccaagataga
     1981 gtaatcttta aattatagaa aggtggatgc aatgagaata acgaaaacta cttttaatga
     2041 tgtattttca agtgatgcaa atgaaagtat tataaaagta gaaaaatatg ttgataaaaa
     2101 atgtgcatct gtatttgaga ttttgaccta cactattatt gttacaaata caaataggta
     2161 caaaactgga aatatatttt tcaaagatta tatttctaaa tatatagagt ttataaataa
     2221 tactgtaaaa gttaatggca ttataaaacg tggattagac ccacagcaag gtttttacat
     2281 agggagaata gatgcaagtt gtaaaaaaat aatatctttt aagagtgtag ttttaccaaa
     2341 ttctgcacat aggattataa agaatagtgc taatatatac tattattata agtgtaatct
     2401 agataaattc ccaacaagaa tatcaataga aagtaataga gtttatacta atgtaaataa
     2461 aattgtattc aaacaattga atataagtaa tactttaaaa gctcctaaaa atctaagaga
     2521 tatattaaaa gtaaatgtaa atagtaaaat atttgatgtt aaacctatga aaaatcttat
     2581 aaacaaaggt agtaatttga aattatgtaa cttgatagtt tttgggtgta tagaagttga
     2641 aatagactat agttgtaaaa ataggagtaa ttttaaaaca gtaaataagt ctaatgacat
     2701 taaaaaagat aagtattata aaaatgatat acaaaataac aatgaaaata taaacgattg
     2761 taaaaagcaa ataagttttg gtgataataa ggtaaaaaaa ataacaaaaa cttttggatt
     2821 ttcttgtttc atttgttctc cagttggaat tatttatgaa gatatgaaaa atataaatat
     2881 aaaaattgaa catacatcaa taaatgaatt aaatccagaa gaattatttg taaatacatc
     2941 acttttattg tattattaag tattttaaag ctaaaactta aacttattaa aatagcattt
     3001 ggttaaag
//
LOCUS       DAMJWS010000176         2960 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMJWS010000176 DAMJWS010000000
VERSION     DAMJWS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.182"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            298..1221
                     /locus_tag="PC399_003701"
     CDS             298..1221
                     /locus_tag="PC399_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF2594499.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1196..2023
                     /gene="panB"
                     /locus_tag="PC399_003702"
     CDS             1196..2023
                     /gene="panB"
                     /locus_tag="PC399_003702"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF2594500.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            2042..2890
                     /locus_tag="PC399_003703"
     CDS             2042..2890
                     /locus_tag="PC399_003703"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF2594501.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1192 a          345 c          465 g          958 t
ORIGIN      
        1 aacatcataa tttttctaat agacttaatc acttttaata tatatagtaa aacaaaaaac
       61 ttatatttta taaatatcat aattttttta ctgatatttt attatataaa ttttatagaa
      121 ttaattgtca attttttatg aaaatggtaa gatatttatg gatggtatct tgttataaga
      181 actatacttt aaatgttttt aattattttg tcttatgtaa ctcatatcct caggaatgag
      241 attagaaatt aagatgttgt atcacaaagt ggaggtgata ctattaaaat aggctttatt
      301 ggttctggaa aagttggaac atctttggca aattattttt tatcaaaagg ttattgtgtt
      361 tcaggctttt atggaaggaa tcaattatct ttattagaat caacaaattt aacaaaaaca
      421 aagatttaca gcaatttgaa agatataatt tatgaaaacg atatattatt tattacaaca
      481 tcagatgatt ctattgaaat tatagacaaa aaactttcca aatttaattt aaaacataaa
      541 tacatttgcc acacaagtgg ctctttaaaa tctagtatat tgttctgttc aaaaaaagca
      601 ggtgcattaa tttattctat ccatcctata tttgcatttt ccaataaaaa taccaacata
      661 aaaaaaatga aagacatttg tttttccata gaaggtgata atgaacaaga tgatttagtt
      721 attcaaaatt tcatagatga tttgggaaac caatttttta taagagataa ggatacttca
      781 tccacttatc atttagctaa tgttttagtt tcaaatttag tattatcttt actagatata
      841 ggagtaagtt actttattaa attaggttta tctgaagagg aatctttaaa agctatcagt
      901 cctttagtaa aaaaaaatat agaaaatata cttgataatg gctttactaa agctcttact
      961 ggtcctgttt ctcgtggaga tataacacca ataagaaaac atctatctgt attggataaa
     1021 aaagatgagg atatttacaa aatactatcc ttaaacctac taaaaataat tgccttagga
     1081 aatgacaatt cattaaaagg tataaaaaat attactatag aaaatgcaat cgaaaattta
     1141 ataagtaaat ctgaaaaata caaagaaatt tacaaacttt taggaggaaa aaacgatgaa
     1201 aaataccata cagactttta agaatgctaa atatgaagga aaaaaactat ctatgcttac
     1261 tgcttatgac tactcaattg caaaaataat ggatgagtgt gatataaatg gtattttaat
     1321 aggagattca ttaggaatgg ttataaaagg tgaagaaaac actctatctg ttaccataga
     1381 tgaaattata tatcatacaa aagctgtaaa aaatggtgta aaaaatgcct taatcgtaag
     1441 tgatatgcct tttctatctt accatgtttc tatagaagat gcagttaaaa acgcgggtag
     1501 acttataaaa gaaggaggag ctcatgctgt aaagttagaa ggtggctcaa acgttataaa
     1561 gcaaatagaa agcattgtaa atgctcaaat acctgtaatg gggcatttag gattaactcc
     1621 tcaatcagta aattcatttg gtgggtttaa ggttcaagga aataccagtg aaacagcaag
     1681 acaacttatt gaagatgcta aattaataga aaaagcaggt gcattctcta ttgtccttga
     1741 aggagtgcct acgaaaatag ctgagatggt aactaattct atttcaattc ctacaatagg
     1801 tataggggca ggaattaact gtgatggaca aattttagtt tatcaagata tgttgggaat
     1861 gtttggagat tttgttccta agtttgtaaa gcagtatgca aatataggag atataatgaa
     1921 agattcaatt aaaaattaca tcctagaagt taatacaggt acatttccac aagaaaaaca
     1981 cagcttttca ataaatgaat ctgagttaga aaaattatac taatattaag gatggtacaa
     2041 aatgcttgta aaagaaatta aattactaag aaatataatc aaagattgga gaaaacatgg
     2101 ctattctata gggcttgtta caacaatggg atttttacat gaaggacacc aaagtcttat
     2161 aaaaaaagct gtaaaagaaa atgataaagt agtagtgagt gtatttgtaa atccaactca
     2221 atttggacca aatgaagatt ttaatagcta tccaagagat atagataaag attttaaata
     2281 ttgtatggat agtggagcaa cagtagtctt taacccatca ccagaagaaa tgtatttaaa
     2341 aggtaattgt actaccatta atgtttctgg acttacagat tttctttgtg gagctaaaag
     2401 acctgttcat tttggaggtg tctgcctagt tgtttcaaag tttctaaata tagtaacacc
     2461 tgataaagct tattttggag agaaggatgc ccaacaatta gctgtaatta aaagaatggt
     2521 caaggattta aacatagata cagaaataat aggctgtcct ataattcgtg aaaatgatgg
     2581 attagcaaaa agttcgagaa atacatatct ctctgaggaa gaacgaaaat ctgctctaat
     2641 attaaataag agcttaagtt tagcaaaaga agaattagta aaaggcaatc taaatccaga
     2701 gaatataaaa gaattaataa cagctaaaat aaattctgaa cacttagcaa aaatagacta
     2761 tgttgaaatt gtagatagtg agacattaca acctgttaag caaatagaac attctatact
     2821 tgtggcaatt gctgtgttta taggaaaaac cagactaatt gataatttta catttgagtt
     2881 aaatatatag tctataattt tatataatat atctaaaaaa taatagaata cctaaaaagt
     2941 aataaattac ctaaaaaata
//
LOCUS       DAMJWS010000177         2926 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMJWS010000177 DAMJWS010000000
VERSION     DAMJWS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.183"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            116..1714
                     /locus_tag="PC399_003704"
     CDS             116..1714
                     /locus_tag="PC399_003704"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2594502.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1949..>2926)
                     /locus_tag="PC399_003705"
     CDS             complement(1949..>2926)
                     /locus_tag="PC399_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HDF2594503.1"
                     /translation="PTGATGPTGEIGPTGATGPTGATGVTGEIGPTGVTGPTGEIGPT
                     GATGPTGVTGSIGPTGATGVTGEIGPTGPTGVTGSIGPTGATGPTGNTGVTGVTGPTG
                     ATGPTGVTGEIGPTGATGPTGVTGEIGPTGATGPTGPTGNTGATGSIGPTGVTGPTGA
                     TGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSITVD
                     ANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGGVL
                     SGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          914 a          489 c          482 g         1041 t
ORIGIN      
        1 tttatataat attttttcac taaatttttt tctttttaca acaaatactc actacctttt
       61 gatataatat ttttatcaat tattattata ttcaagtttt gaaaggagta ttaaaatgag
      121 tttattagtt ttcggacata aaaatccaga tacagattca atatgttctg caatttcatt
      181 agcacattta aagaacaaac taggaataaa agcaacagct tatgctttag gagatatcag
      241 aaaagaagct aaatatgcct tggattactt taaggtagat gcacctgaaa ttttagataa
      301 tgttagaata caagttagag atttaaatta tgataaagta gtacctttaa caccaacttc
      361 atctatatta gaggcttata accttatgga tgaaaaaaat gttaaaacac tacctgtaac
      421 ttatgatgat ggaacttttg ctggtatcat tactatgaaa gaaatagcta aaaatctact
      481 tcatcaacac tttacaacta tacacacatc actatcaaac atttgtaaaa accttaatgg
      541 tactattctt gtggactgtg gtgaagatat aaaaggtaga gtagtcactt tatcatttgg
      601 aatggagact ctaatagaga ctttaaaaga aggagacata gctattgttg gagatagata
      661 tgatagtatt gaatatgcta ttgatacaaa agttaaactt ttaatactta ctaatcatac
      721 agagatttca aaagatttgc tagctcttgc taagataaat ggagtgtctg ttgtttctgt
      781 tgaaagtgac aattataaaa cttctaatgt aataaatcaa tgtagtttct tagactctat
      841 agaagctaca gaaaatttaa ttacattcaa agaagatgat tatatggatg aaataaaaga
      901 gattatgcta gaaacaaact tcagatctta tccaatatta gatgatgata ataagttttt
      961 aggtttagtt tcaaaaggtc atctattaaa cccatctaaa aagaatgttg ttttagttga
     1021 ccataacgaa tatgctcaaa gtgctgatgg aattgaacaa gcaaacatag ttgaaataat
     1081 tgaccatcat aaacttggcg gtatttctac tgatgttcct atgtcattta gagttatgcc
     1141 tgttggatgc aatagtacta taatttatca aatgtacaaa gaaaataatg ttgagatacc
     1201 ttatgaaata gctgggcttc ttttatctgc tatattatca gatacattat tatttaagtc
     1261 tccaacaaca actgatatgg ataagaaagc ttgtgaagaa ttaagtaaaa tagcaaaagt
     1321 agacatggaa aaatacgcta tggatatgtt taaatgcggt acttctttag atgaatatac
     1381 tatagaagaa atagtaaata tggactttaa agaatttaat atgagtggac atagagttgg
     1441 tattggacaa gtatttacct tagacataga ctctatattt gctaaaaaag atgagttttt
     1501 atcttatata aattcgactg attatgataa attaattctt tcagttacag atataatcaa
     1561 agaaggttct tatttaatct ataaagcaga agatagctta attgctaacg catttggtgt
     1621 tgaaggtgtt caaggcacat ttgcaccagg tgttgtatca agaaaaaaac aattagtccc
     1681 taatttaact actgcaatca aaaattttaa ataatataaa atttttaaat aacgaaaact
     1741 gtctcaaatc tatatttgag acagtttttt attatatata tattgataat aaagttaaat
     1801 aaaaatccat aaatttaaaa tttttatgaa tatattttat tattattgtt attttattca
     1861 ttctaaaata ggtaaaactc aatatataaa aatacttttg tcatttaaat aaaacctaaa
     1921 tagtttttaa gattatttta gacacgtttt aagtcataac agtatcagct attctaaata
     1981 tcgtcaaagt agccgcagtt cctgctgatt gcccatttcg tatccctact gttgtagatg
     2041 aaaagttatt tattgtaaaa gtagtcccac caaataaaaa accagcaaaa tatccagaaa
     2101 gtactcctcc gcctcctggc tcagtagtaa gccctgccaa atcttttcct cctaaaattg
     2161 ggaataggat agcgaaagaa tattgattta tacttgatga acctggttgt aatgctactg
     2221 aaactgatat ataatacaca ccattttctt gtactgtaaa cgtaccattt gcatctactg
     2281 ttatacttga actatttcct attattgttt gccaacctgt tatagcatct ccattattta
     2341 gtgtttgtgg agatggtgca tttacgagga agttagcaac tggctgagag gaattgcctg
     2401 ttgctcctgt tggacctgtt gggcctgtta ctcctgtcgc tcctgttgct cctgttggac
     2461 ctgttactcc cgttggccct atacttcctg tcgctcctgt atttcctgtt gggcctgttg
     2521 gtcctgttgc tcccgttggt cctatttctc ctgttactcc tgttgggcct gttgctcccg
     2581 ttggtcctat ttctcctgtc actcctgttg ggcctgttgc tcccgttggg cctgttactc
     2641 ctgtcactcc tgtatttcct gtcggtcctg ttgctcccgt tggtcctata cttcctgtca
     2701 ctcctgttgg gcctgttggt cctatttctc ctgtcactcc tgtcgctcct gttggtccta
     2761 tacttcctgt cactcctgtt ggtcctgttg ctcctgttgg tcctatttct cctgttgggc
     2821 ctgttactcc tgttggccct atttctcctg tcactcctgt cgctcctgtt gggcctgttg
     2881 ctcccgttgg tcctatttct cctgttgggc ctgttgctcc tgttgg
//
LOCUS       DAMJWS010000178         2842 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMJWS010000178 DAMJWS010000000
VERSION     DAMJWS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.184"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            445..1488
                     /locus_tag="PC399_003706"
     CDS             445..1488
                     /locus_tag="PC399_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594504.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            1599..2741
                     /locus_tag="PC399_003707"
     CDS             1599..2741
                     /locus_tag="PC399_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2594505.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         1280 a          227 c          494 g          841 t
ORIGIN      
        1 catttttgtt atttataatt attattgaat atatttttac atataagata taaggtgata
       61 gatagaaatt gttggattat gtaaataatt aatatataat accattatgg gcaataaata
      121 caaatcaaat gataatatag tctatttatg taaatgttat attgtatact aagtataata
      181 gtaagttttt tgatagtggt gtagaaagta aaaatctact agcctaaatt gatgtatgga
      241 ttagtttagg aacagtaatt gcatatctta agtttgatgt catgttgggc tactagaaat
      301 aggtcatctt tttcacttga atatcttttc tttaaacatg gagagtatca tatcaaaaac
      361 atatgaaaat taagtgattt ataagaacag taatatttta tatgatagta tagaatagta
      421 ataaagtatt aggaggaaca ttgagtgtta aaaaagtggt ttggtattgt gaaaaaaaga
      481 caaaaaagtg agtcagcaaa agaagaaggt gaagtaatat taaaaaacga aaaaatatta
      541 tctgaagaaa agttgataga tgaagaagga gttgtagtta atattgataa tgaaatatta
      601 actaaagtag aagtagtaaa tgatgataat gaaatagaag aaaaaataat agaagaagat
      661 tggttaatta gtgaaaatac cataaaatta gatgataaag aagcaattat taatgataaa
      721 aacatagaat tatgtaaaaa agaagttcaa gttgaaagtg aaaaaatagg tttaaataag
      781 tttgaaggac ttgaccaaaa tgaaaattat aatctagaaa aaaatgttat tgaagaaaaa
      841 aaagtaagtg aatgtttgac agaagaagat ttagagtaca taaaagaaat taaaataaaa
      901 agaggaaaaa gtataaaagc tataaacttg tatactaaag aagagtgggt ttttgacact
      961 catatacagt gtagtaaaaa actcaaagtt ccattagggt acataagaga aaatttaaaa
     1021 tatggatata tggattactt tggagatgca ataaattatt taagtgaagt attaaatata
     1081 gatgaatact gcaaaagtga atggagctat ctcgataata gcaaatctcc atctgagata
     1141 tttaatattc taaacaataa aatatttagt ataaggcttt ctaatgaaaa aagaaatgaa
     1201 attttgacaa atgataaaat tgaagcatta aaaatgaatt atagatttga atgtattgat
     1261 gaagaatatg atgaatattt taaaaaatat aagtctataa tcaaaagagg tggaaagaaa
     1321 aaagttgaat tagtaaataa aaaaggtgac attttagaaa tatttaagtc tttagaagaa
     1381 tgtgccattt atttgcaaaa ggaaaagaat gaagttatac aaatgttaaa atatggagat
     1441 acaaaagtag gaagaaactt tataaggtat agtttgagaa gtatttaatt tcattttaaa
     1501 ttcataagag tttcatttct gattcacaca tttaatatta ttttaatgta aaataataaa
     1561 ggtgaagtat tgcacaatta aaattaagga gggtattaat gtctgaagaa aaaaatccaa
     1621 aagaaaatac attagatgaa tctaaaaatt cagataatgt aaattctaaa aaagatgatg
     1681 tgtcagatga aaaaaatact gataacaaag ttaaaaaaag ggaaataaag ctaaacaaca
     1741 aaacacttaa agctattgta gcaattgttg cagtaggagc tatatgttta ggtggaggct
     1801 atgtttttgg caaagaagct ggaagaaaat tgcctgctac ttcaaaacac tataatagca
     1861 gtgaggtagt agctactatt ggggataaaa aaataacagg tgaaatgtta agaaagagaa
     1921 tggagccatt attttattta aatgcaaaaa ctactatgac tgatgacgaa atacgttcat
     1981 atgaatcaaa tatgattgaa actatgatta catctgatat attatcctct gaagcagcaa
     2041 aagagaaaat aagtataact aatgaccaac tagatgctca gtataataat ttaatgagta
     2101 gtatagaatc agcaatggga atgactaaag aagagttttt aaaacagttt gatttgactg
     2161 aaaaatatat aaaagatgat atgaaaaaag agctagtagc atcaaaatat ttaaatgaaa
     2221 aatcaaaagt tactgataaa gaagcagaga actattacaa taaaaacaag agcaattatt
     2281 tacaagttag agcttcacat attttaataa aaactgttga tgataaaggt aagcaagtta
     2341 gtagtagcaa aaaagctgaa cttaagaaag aagctgaaga aatacttaaa aaagcccaag
     2401 ctggggaaga ttttgctact ctagctaaaa aatactctga agatagtagt gcagaatcag
     2461 gtggagattt aggtttcttt ggtaaaggtc agatggtgga atcttttgaa aaagcagcct
     2521 ttgcattgaa gaagggagag gtatctaaca aacttgtaga aagtgattat ggatatcata
     2581 taattaagaa aactgatgaa aaatatcagc catttgaaga ggtaaaatca gatttagtaa
     2641 gcagtttaac atcagaaaaa caaaacctac ttatacaaaa tctaaaagaa aaatataatg
     2701 ttaaaattac agataaatat caaatgaaag ctaaagaata gataaagtca atttattaaa
     2761 aaaaagaaaa attaactgta tggataaaag ctatttttac tcttaatata gagagtataa
     2821 aatagctttt attattttgc aa
//
LOCUS       DAMJWS010000179         2814 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMJWS010000179 DAMJWS010000000
VERSION     DAMJWS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.185"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            116..2251
                     /gene="rnr"
                     /locus_tag="PC399_003708"
     CDS             116..2251
                     /gene="rnr"
                     /locus_tag="PC399_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF2594506.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1235 a          263 c          528 g          788 t
ORIGIN      
        1 ttataataat aagaaaatat agaaaattat gtgataagta aaaaatgttt tgtctgaaga
       61 aatataaaag acaatataaa taataatata atgaaaagta aaacaggagg gaaaaatgat
      121 accaggactt aaagaaagtt tgctaggatt aataaatgaa agtgcatata atcctctaaa
      181 aaaagaagaa ttagcagaaa tatttgatat acattcatca gaaatgccaa tgttttataa
      241 ttttttaaat gaattagaag aagatggtta tatctgcttt actaaaaaag gaaaaatagt
      301 atctcccaat caaatgggat actttgttgg aaaatttgta tcacacaaaa agggttttgg
      361 atttgtagaa tctgatgttg aatatacaca ggatttattt atctcaagtg acaatataaa
      421 tggtgctatg cataatgata gagttatggc agaaatagta gtacctgcaa ctgaagaaaa
      481 gagagcagaa ggaagaatag taaaagtaat aaagagagaa ataactagaa tagtaggtac
      541 cttccaatca agtaaaactt ttggatttgt aacaccagac aacaagaaat ttactaaaga
      601 tatatatata cctaaaaaac attttagtgg agcagtagat aatgataagg tagtatgtga
      661 aattacagta tggccacaag aggatagaaa accagaaggc aaaattatag agatactagg
      721 tcaaaaaggt gaaagaggcg tagaaataga ttctataata agagaacatg gtcttccaga
      781 agaatttcca aagaaagtat tgcaagaagc agaagctgta gctgttgaga ttcctcaaga
      841 agagataaaa agaagaagag atttaagaga tttaaacata tttactatag atggagatga
      901 cgcaaaagac ttagatgatg ccatatcaat agaagtatta cctaatggaa acttcaaact
      961 aggagttcac atagctgatg ttacacatta tgtaagagag aaaaataaac ttgacaagga
     1021 agccttaaaa agagctacat cagtttattt agtagataaa gtaattccaa tgttgccaaa
     1081 aactctttca aatggagtat gtagcttaaa tccatttgaa gataagctaa ctctttctat
     1141 atttatggaa atagaccata aaggaaatgt ggttaaacat gaaatatgtg aatctgtaat
     1201 aaattcaaaa gctagaatga cttatacaga agtatctgat atattagaaa aggatgatga
     1261 aaaacttaaa aagacttttg aacatgttgt tgaagatttt aaaaatgctg aaattttagc
     1321 taggattctt atgtcaagaa gggaaaaaag aggagctata gacttcaatt tcccagaggc
     1381 aaagataatt ctaaatggaa aaggcgaagt tgtagatata aagccatatg aaagaagaat
     1441 atctaataag ataatagaag aatttatgct tataagtaat gagacaattg ccgaacattt
     1501 ttactggatg ggaataccgt ttgtgtatag aatacatgag acaccatctt tggaaaaaat
     1561 ggaagaattg agtaagttta tatctacatt tggatatata ataaaaggtg ataaagagga
     1621 agtacatcca aaagcattac aaggtataat agagaaaata aagggcaaaa aagaagaagg
     1681 agctatcagt actataatgc ttcgttcttt aaaacaagct aagtactcac cagaatgtag
     1741 tggacatttt ggattagcag ctaaattcta ctgtcacttt acttcaccaa taagaagata
     1801 tcctgacctt caaattcata gaataataaa agaatcttta aataataaaa taagtggaaa
     1861 aagacaagaa cagcttacta ctatagtgga ttatgcatct gttcaatcat ccgagaaaga
     1921 aagaaaggca gagttagctg aaagagatgt acatgatttc tataaggctc tttatatgaa
     1981 ggataaagtt ggtcaagaat ttgagggtgt tgtatctagc gtaacttcat ttggaatatt
     2041 tgttgagtta gataatacca tagaagggtt gataagactt gcaaatatga atgatgatta
     2101 ctatatttat gatgagagtt cttatagtgt tttaggagaa agaactaaga agtcatataa
     2161 gataggtgac cttgttaaga taaaggttga aagtgttaat gtagatttta aagaaattga
     2221 ctttgagata gttgaaaaaa tagaggagta gataaaacag ctaaaataag agataacaag
     2281 ttgtagtatt tatgatatgt ataaatatat agtgcacaat actctataaa ataaatgata
     2341 aaaataggta attgagaaat atctaatgga aatagttaac tgaaagataa ataattaaga
     2401 aatatttaat gaaagtagct aattgaaaaa taagtaattg agaaatgttt aatagaaaga
     2461 gctaactgaa agataagtaa ttaagaaaca tttaatagaa aaagccaact gaaaaataaa
     2521 taattaagaa atgtttaata gaagtggcta attgaaaaat aagtaattaa gaaatgctta
     2581 atagaagtgg ctaattgaaa aataagtaat taagaaatgc ttaatagaag tggctaattg
     2641 aaaaataagt aattgagaaa tgcttaatag aagtggctaa ttgaaaaata agtaattaag
     2701 aaatgcttaa tagaagtggc taattgaaaa ataaataatt aagaaatgtt taatagaagt
     2761 gactaattga aaaataagta attaagaaat gcttaataga agtggctaat tgaa
//
LOCUS       DAMJWS010000180         2755 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMJWS010000180 DAMJWS010000000
VERSION     DAMJWS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.186"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            339..2150
                     /locus_tag="PC399_003709"
     CDS             339..2150
                     /locus_tag="PC399_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2594507.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2238..>2755)
                     /locus_tag="PC399_003710"
     CDS             complement(2238..>2755)
                     /locus_tag="PC399_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594508.1"
                     /translation="QSIYKFIAKIPFVKKDNSNFNYTNYYMEFEDIPEYYEVNFNNYL
                     YIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFINGETLVDSYNFPNNEVKDYIQK
                     RESLYIETKKYYEEKGLKIERHALGSEDGEYVELTNDSILYLLDIEAVEIREQSKNIE
                     EFLYKYKKRFK"
BASE COUNT         1040 a          287 c          399 g         1029 t
ORIGIN      
        1 aaaatataga ttatgtatat gccaaattta aaaagagaca taaagacatt atacaaatat
       61 attattgggg tgatatttta taagaaaaga agtgatgcag taaattaatt gagcaattta
      121 aaaatgattt aatgatagtg ttgatgaatg tttaaaaaga aattttagaa aaacgtatat
      181 tgctgtttgt gttggaggtg atgaatttgt ataatttata ggatataaat gattaaacta
      241 tttaagataa atacatatca ctgtaaaata ttgataatca ttttttataa gagattgatt
      301 atttgcaaat tatatatatt acaaatgagg taacaataat gaagaaaaaa tatgattttt
      361 taatttattc tgctatttgg ctagttatta ttattctttt tatattttct ctatatcaca
      421 gtatagagca tattaaaatt ataagtcaca ctggtactat tcgcagtgaa actcaaaagg
      481 ttgttaagca agaattgaat aatgaaagaa atgatgactt gataaaacga ctggataata
      541 ttctgataaa gctaagaaca ggtaatggag aaaatggatt tcaaagatgt gataataaag
      601 aatttcaaca aaaactaaat caaatggatt ctatgtggga atctatgaag aaagaaatta
      661 taaaagttag aaatggtgct tcaggtgata aattatataa attgagtgaa gagtattctg
      721 tgttgtctaa tcaaattgtt tttatatctg aaaaacactc taatgcaaaa ttgtattctt
      781 ttgctactgc actatttatt tatcttatat tctcaacaat aagtctttta atttgggaat
      841 attataataa aaaaaggttt aaaagaatat tttatactga taatttaaca aaaataaaaa
      901 atcaggttgc atttgaaaac agagcgattg aaattttgca caatgcatct aacaaagagt
      961 atgtactgct aaatattgat attgacaatt ttaaatatat taatgataca catggatatg
     1021 aatatggaga taaagttcta ataatagttg cagctgcact ttctaaaact tttaatatca
     1081 aagaaacttg tgcaagaatt ggttcagata attttgtaat acttgcaaaa tatagagatt
     1141 ctcttttaga ggatattcgg gaaatgctaa ctaatgctat aatctctgaa ttagatatga
     1201 atgtaactca aactatatct tattgtatag gagcgtactt agttgaaatt gataatttag
     1261 gttataaatc catcaattct ataatggata aggcaaatat tgcacacaag gtaagtaaaa
     1321 caagaggaat ttcctccaca gtatggtata atgaaaatct gttaaaacag ttacaaatgg
     1381 aaaatagtat ttataattat atgtataaag cgatagaaaa tgaagaattt catatgtatt
     1441 tgcaacctaa atttcaaata tcttctttaa atgttgtaag cgcagaagct cttgtaagat
     1501 ggttttctcc agaacttggt tttttatcac cagatgaatt tattccattg tttgaaaaaa
     1561 gtggatttat tattgaattg gacttttata tgttaaaaaa agcgtgttca tttgtaagaa
     1621 aaacgtttat gaaaaaaaat caatatacat atccgattgc agttaatttt tcaagggtta
     1681 ctatttacca aaatagtttt tatcaaaggt ttttagatac tgttaaagaa tatgaaattc
     1741 cttttaagta tatagagata gaggtaactg aaagtgcttt taatgaaata tctcaaccag
     1801 ttatttcaat tttagaggaa cttaaaaagt taggattttt aatttcaatg gatgattttg
     1861 gttcaggata ctcttcatta agtcttttat gttctctatc tattaatgga ttgaagttag
     1921 ataaaagctt acttaaagaa acctttaacc gggagaaagt atatagtatt attcaatgta
     1981 ttattgaaat gtcacatcgt ataggcatgt ctgttgtatg tgaaggtatt gaaactaaaa
     2041 aagacttgga atttttaaat acagttaaat gtgatgtagg acaagggttt tatttttcta
     2101 aaccgataga agaaaaagaa ttttttaaca aatatgtaac taaaaaataa tataattatt
     2161 tttatcagtc tatacattga gtcattttat atctcgttaa atgactcaat gtactgttat
     2221 gtattttaaa atataaatta tttaaaacgt tttttatact tatatagaaa ttcttctata
     2281 ttttttgatt gttctcttat ttcaactgct tctatatcta gtaaataaag tatagaatca
     2341 tttgtaagtt caacatattc accatcttct gaaccgagtg catgtctctc aatttttaat
     2401 cctttttctt cataatattt ttttgtttct atatataatg attctcgctt ttgtatgtag
     2461 tctttcactt cattatttgg aaagttatat gaatccacta atgtttcgcc atttataaaa
     2521 gttttctcgg tagcaacttt ttcatcccaa caatggattt cttcccaagt aaaaaatatc
     2581 tcagttccat ctggaatgaa tgaaaacctg gtaataatat ataaataatt attgaaattt
     2641 acttcataat attcaggaat atcttcaaat tccatatagt aatttgtata attaaaatta
     2701 ctattatctt tttttacaaa gggtattttt gcaataaatt tatatattga ttgtt
//
LOCUS       DAMJWS010000181         2617 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMJWS010000181 DAMJWS010000000
VERSION     DAMJWS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.187"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            262..2280
                     /locus_tag="PC399_003711"
     CDS             262..2280
                     /locus_tag="PC399_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2594509.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1087 a          306 c          428 g          796 t
ORIGIN      
        1 ttgtattaaa tttaataaac ctacaattgt tatatgtttt tatttgtctt ttatagattt
       61 ttttggcaat aaatgatata gtgaagataa ttaaatatat tcaatatatt taattttttt
      121 attttttgtt tttataattt ttttagaagt ttatttttta tgtacttatt tataattttg
      181 ggtaaaaaca ctttgatata atttcaaaaa ttaaaaggtt aaaaaacaaa gtagaaattt
      241 atgttaaata aggagattaa tatgagaaga aatacaaaat tattaacaac agggattctt
      301 tcaatggcaa tcgtcgcacc tacaatggca tttgctactg aatctaatgc tatggaaaat
      361 aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag tacctcaaaa
      421 gtcagtgtta agtttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc
      481 tatgatatgc cattggatac aactttaaac tacaatcaat caactgagtc atatgaagga
      541 actatcaatt ataataaaga cccagaatat ctaaatgttt gggaactaca agggataaca
      601 ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa gatgggatta
      661 aatttaaaag actataatgt aacacaggaa tgtataattg aagatataac ttctagaaaa
      721 gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac aggaagtgat
      781 agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc agataaggta
      841 gttataataa atggggatgt aagtatagat ggcattatat caactccact agcaactaca
      901 tataatgcgc caatactttt ggttgaaaaa aacaatgtac ctaatagtgt aaaatcagaa
      961 ttaaagcgtt taaatcctag ggatgtaatt ataattggag atgagaatgc tatttctaaa
     1021 actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct aaagggctct
     1081 aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa
     1141 aaagtgtaca taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa
     1201 gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac agacaacaca
     1261 tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg ccctcaaatg
     1321 atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt tactaataat
     1381 agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca
     1441 gatgatgagc tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca
     1501 gcaggtccat tggctgccaa cttaaaatct ccaatactta taacaccaaa gacgtatgta
     1561 tctgcatacc ataaagaaaa cttagaagct aaatcggcta ataaggtata taaaatagga
     1621 ggaggattga cttctaaggt aatgaactct atagcatcat cattatctaa acacaatacg
     1681 actccaacag aaccaggaaa tagtgggggt aagacagtta tgattgaccc aggacatggt
     1741 ggttcggaca ctggaacaac aggtaaacca ttagggggta ttaaagaaaa agactataca
     1801 ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa tgtaataatg
     1861 acaagagata cagataagac tttatctctt ggaaatagaa ctgctttatc taactctttg
     1921 agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg gaatggtgta
     1981 gaagtatttt ataagcttaa agataaagat ggaggaacta ctaagactgt agctacaaat
     2041 atattaaata gaatcttaga gaaatttaat ctaaaaaata gaggtgcaaa aacaagaaca
     2101 ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga tatgccagct
     2161 gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact taatacatca
     2221 aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc attaaaatag
     2281 tctacaatga gagcatagaa aaatttaaaa taaattaatc tataatttta aatttaaact
     2341 aaagctataa gataagaatg taattataat agtaataaag actgtatata cattgtgcat
     2401 ataaatgcga aatgaggtat atacagtctt tttataatta attgtaaaat gatttatgtt
     2461 atatgaaaaa tttacatata ttaaggctac agttaattgt caagagctgg agtattcatt
     2521 tgtaatacac aatataagct taattactat attttattgt gtgttttttg tggattttta
     2581 tgtcaataaa tgatatagta tagataatta aatgtat
//
LOCUS       DAMJWS010000182         2561 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMJWS010000182 DAMJWS010000000
VERSION     DAMJWS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.188"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            106..579
                     /locus_tag="PC399_003712"
     CDS             106..579
                     /locus_tag="PC399_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2594510.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(948..1505)
                     /locus_tag="PC399_003713"
     CDS             complement(948..1505)
                     /locus_tag="PC399_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2594511.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSFL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            1763..2461
                     /locus_tag="PC399_003714"
     CDS             1763..2461
                     /locus_tag="PC399_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2594512.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNIPTKILYGKHDELTEIGVVEKFVEKFNCNLTIVENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          961 a          326 c          304 g          970 t
ORIGIN      
        1 tatatattat cataatattc taaatttata aaacaaattg tattgaattg aatttattta
       61 agatatataa ttataattaa aataataata aaggtgttgt ttataatgtt caaagaatac
      121 ttaataagag aattaaaaga agatgatatt aaaaatgcat tagacttaat ctggaacgta
      181 tttgaagaat ttgaatctcc agattattgt gatgaaggaa ttacgacatt tcatcaattt
      241 atagaatata actctattat tgaaaaaatc aaagaaaaaa ctctcttctt ttggggttgt
      301 tttgactcta aaaaacttat tggagtctta gctattagaa atttaaatca tatatgtatg
      361 ttatttgtag ataaaaacta tcataaacat ggtgttgcaa aaaaattaat gactacaggc
      421 atacaatact gtatttgtaa taaatttaat ttaactgaaa taactgtaaa ttcatcacct
      481 tatgctgttg aagtttatca taaattagga tttaaagata ctgctgttga gcaaattgtt
      541 gacggtatac gttttacacc tatgaaattg ttaatataat aaattaaata aaaagcaaag
      601 tctcttaaat attttatagt tactttgctt ttaaaccaag atttaaaata atactatctt
      661 ttttgttttt taataatata ctttaaaatc cttactaata tgaactataa tttatttttt
      721 gatatccttt ttgattaaca tataaattaa ttcaattatt aaacataaac tatgttgttt
      781 ctctttatat ctcttctttg taaacaatat tgggcatttg aaaataaata ttcttaaaat
      841 atactgttct cctgttgatt ttcctttggt acaaatgata aaaatttaat ttacatgccc
      901 cataatgaag tgttcaataa ataagttaaa ataatttatt tacctcctta atcatatttt
      961 ttatgaatac ctaataacac agtatcaata gaatcgacta aaccttttat agcttcttct
     1021 ttactcaaaa atgatttccc aagttgcaaa gtcgaataac catgaagtac actcattatt
     1081 aagtttaata cttcacctgt attctttgta tttagattac aagaaagaat aagtttttct
     1141 aataaggatt tatattcatc aaagatttct aatgtttcat tatttccatg ccaagtagcc
     1201 cactgaattg tttcataaac accaggatta gaaattataa aattaaagta tgctatactt
     1261 atacacttta tagctgtatc tccaaaattt ccaatagccg cctttatcat ttgactattc
     1321 atctctctca ttcctttatg agctacttca agtaataaat catctagact ttctatatga
     1381 ttgtacaaag atggtgtacg tattcctagc ttttctgcaa taacttttaa agaaactttg
     1441 ttaagcccat ctttattaac tatatctgat gtagcttgta taactatttc tctactaaca
     1501 cgcatgattt tccctctttc atttaaaatt aattacttta ttagtttaac taatctaatt
     1561 agtttcgtca attattgtaa tttataaaat tctttatact attataaaat cttgacagtt
     1621 taattataat ttaatataat actaattaaa ttagttttaa tactaatata attagtatta
     1681 tattaaattc tatattacaa cattatttta tagtttgatt aatttaataa tcactaatat
     1741 atcaaactga aaggagattt ttatggaaaa aaaatatttc gaaattcatg gagttccttc
     1801 aatcctttgg ggaaatcctt caagtaaatt atatatttac atacatggac aatgtggcaa
     1861 taaagaggag gctgaagctt ttgctaatat tgcagttcca catgagtgtc aagttttaag
     1921 tgttgatttg cctgaacatg gagaacgtaa aagagaaaaa aattcattta acccatggca
     1981 tgttgttcca gaattagtat ctattatgga atatgcaaag tgccactgga atcaaatttc
     2041 gttatatgca aatagcattg gagcatggtt tagtatgtta agctttgaaa aagagaactt
     2101 aagagagtgt atttttgtat ctccaattct agatatgcaa cagctcattt caaatatgat
     2161 gctctgggca aatgtatcag aagaacagct taaacatgaa ctaattatcc caacttcatt
     2221 tgggcatact ctttcatggg aatatctaca atatgtaaaa caacatccta tcactcaatg
     2281 gaatatacca acaaaaatat tatatggtaa acatgatgaa cttactgaaa tcggtgttgt
     2341 agagaaattt gtagaaaagt ttaattgcaa ccttactatt gtagaaaatg gcaaacattg
     2401 gtttaacaca ccacaagaat taaattcatt atatgaatgg ttaaatactt tagttaaata
     2461 aaaaataatt atattattaa tatgattaaa ttatttataa atgttattta attaatttta
     2521 ttttagcatt attaaaataa ctacataaag aataaattag t
//
LOCUS       DAMJWS010000183         2464 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMJWS010000183 DAMJWS010000000
VERSION     DAMJWS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.189"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            317..1843
                     /locus_tag="PC399_003715"
     CDS             317..1843
                     /locus_tag="PC399_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2594513.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1871..2428
                     /locus_tag="PC399_003716"
     CDS             1871..2428
                     /locus_tag="PC399_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594514.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
BASE COUNT          968 a          214 c          440 g          842 t
ORIGIN      
        1 attacatatc agaaaaatat taaactaact aaatcaataa aaaagttggt ggataacaat
       61 ttataattgt tagcacaaca tcatcttatg tattgaaatc taaaatatcc tcatttttac
      121 ttagcataaa tactattaat taagaaaata atatagaaat tataaatata tataagttta
      181 gtctggtgta taaaacaaga aaactaagtg atttttaatt taacttttta ttaagcttat
      241 tttttatact atttataata aataaactcc atgatgggaa aactattact atttaatatg
      301 taaaggagta ttttaaatga atgaatcata ttggttttta aactcaagtc caaaagaata
      361 taacaaatta ggtgaaaata taaaaacaga ttgtcttata gtaggtggtg gaataactgg
      421 attgacaact gcatatttat tagcaaaaga aggtaaaaaa gtagtgcttg ttgaggctga
      481 taaaatagga tatggaactt ctggaaggaa tactggtaag gtaacttgtc agcatgatat
      541 attttactct aagatagaga aaaaatatgg attagataaa gcaaaatcat attataatgc
      601 aaataatgaa gctttaaatt tagttgaaca aataatagaa gaaaacaata taaaatgtga
      661 ttttaaaagg gaaacttctt ttgtatttac agaaaaagag gatactataa aggatattaa
      721 agatgaatat cgtacatgta aaaaaatagg aataaattgt gagtatcatg aaactataga
      781 aaatattcca ttagatataa agggggctat tagttttact aatcaaggtc aatttaaccc
      841 aaagaagtat attgatggat tggcaaaagc agcagtaaat ttagggctaa aaatatatga
      901 aaatacacca gtagtagacc ttgaaaaagg aaaaatatgt agggttaaaa caagagagga
      961 taatataata gaagctgaga atgttataat ttcatctcat agtccatggt atgatggatt
     1021 gaatttttat tttgcaaaag aatatgcaga gcgtgcttat ttaatggttg cagttttgga
     1081 aaataaatta gcagatggta tgtttataag tatagatgac ccatcaataa cttttagaca
     1141 atataatgat ggaagtgaaa atttactgat ttttggagga ggagaccata aagttggtca
     1201 gggaggaact gagaaagaaa tatttgatga tttagagcat tatggtaaag aagtctttaa
     1261 ggtaaaagat tttaagggga aatggtctgc tcaagacaat atgagttttg acaatgttcc
     1321 ttatattggt tatatcaaca aaagagaaga caatatttat gtagctacag ggttctctaa
     1381 atggggaatt actaatggaa ctgctgctgg aataataata aaagatttga taataaataa
     1441 taattctgat tataaagata cttttaatcc atccagatta ggtagctact tttcaaaaga
     1501 ttttataaag gaaaatgcaa acgtagcaat aaattatgta agtggaaaat taaaaatagg
     1561 tagtggagat atgcctaaaa ataatggaga aggaaagatt gttaatattg atggaaaaag
     1621 atatggtgta tataaggatg ataatggaga tttttatatt gtagacacta catgtactca
     1681 tttaggatgt gaacttaatt tcaacagtga agaaaaaaca tgggactgtc catgtcatgg
     1741 ttctagattt gactataagg gaaatatact tgaaggacca gctcttaagc cattaaaact
     1801 ttatggacat ggtgataatg atgttaatcc taagttatta taaaattatt tttaggaagg
     1861 taaagtgtat atgaataaac ttttaaagcc aataactata ctattgtata tagtttatat
     1921 tttatctgcc gtattttttg tagcaaacaa agaatatgga agtctaggtc tggtagcaat
     1981 tagtttagtt ggtactttca ttttgagttt tataaataag caaacggaaa gattattgag
     2041 taaagaatta tatataatgc ttgctttatt tataatgttt tcatcattgt taggtacttg
     2101 ttttaatttt tatgatataa attattatga tgattttttg catttatggt caggtataat
     2161 atcttgtatt gtggcttttt ctataattaa atacttttat acacaaagag agataagtaa
     2221 aatgagtaaa gtgtttttag ttatattttt atttatgttt agtatgggag tagcgagttt
     2281 atgggaaata ggtgaatttt taatggatac ttttgttggg actactacac aggccggagg
     2341 gttagaagat actatgattg atatggtaga tgctttaata ggcacaatta taactattcc
     2401 ttttatagta aaaaaatcta atatatagtt ttaaattttt tatattaatt ataatggatt
     2461 taat
//
LOCUS       DAMJWS010000184         2444 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMJWS010000184 DAMJWS010000000
VERSION     DAMJWS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.190"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..581
                     /locus_tag="PC399_003717"
     CDS             <1..581
                     /locus_tag="PC399_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2594515.1"
                     /translation="HIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKT
                     IGSLNRNKARLKVARFQEHIANQRKDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKL
                     SRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREW
                     ICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            714..2219
                     /gene="ade"
                     /locus_tag="PC399_003718"
     CDS             714..2219
                     /gene="ade"
                     /locus_tag="PC399_003718"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF2594516.1"
                     /translation="MSPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADPHELVNV
                     KGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNNPRILGL
                     GEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDHECITFD
                     EVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDIEKQGHI
                     RWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLDKMEVDS
                     VYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEIVPYQIL
                     TNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAVAPLKGFGIKNGAIATTVAHD
                     SHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFTAEEVQE
                     ITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT          959 a          263 c          452 g          770 t
ORIGIN      
        1 atcatatagg tttagattta ggaattaaag aattttgtat ctcaagttgt ggagacttta
       61 tagaaaatcc taagtatctt aagaagtcat taaacaaact tgctaaatta caaagagaat
      121 tatcgagaaa aacaatcggt agtttaaata gaaataaagc aagattaaaa gttgcaagat
      181 ttcaagaaca tatagctaat caaagaaagg attttctaca aaaattatct actaaattaa
      241 taaaagagaa tgatattatt tgcatagaag atttacaagt aaagaatatg attagaaatc
      301 gtaaactatc tcgcttaatt tctgatgtgt catggtcaga gtttattcgt caattagaat
      361 acaaggctaa ttggcatggt agacaaattg taaaggtagg taaattcttt gcaagttcac
      421 aaatatgcaa taaatgtggg tataaaaacg aggaagtgaa ggacttaaat ataagagaat
      481 ggatttgtcc tagttgtaat gaaactcacg atagagatat aaatgcaagt ataaatatat
      541 taaaagaagg attaagacta ataacaattc aaaataaata acgtatgtaa gaaccgtagg
      601 aactacgggg atagcctgtg gagaattagt aagacatatt ttagtatgca aaattctgtg
      661 aagcaggaac cctgtgactt tagtcatggg aggttcagtg tagcaataaa tatatatcac
      721 ctggctttat tgatgcacat atgcatattg aatctacaat ggtaatgcca attgaattat
      781 cgaaaaaatt attaaaatca ggtacgacaa ctatcatagc cgacccacat gagttggtca
      841 atgtaaaagg agttagtgca atagattttt tattagaatc gacaaaagat ataccactta
      901 atgtatatat tatggtgcct tcatccgtac ctgcaacaag ttttgaaact aatggagttg
      961 gtaaatttag tgcaaaagac atggagagtt atgtaaataa tcctagaatt ttaggtttag
     1021 gagaggtaat gtgttttaat gatgttataa attctgaaaa tgaaatcttg gataaactag
     1081 agctttttaa aaataaagtt gtagatggac atgcacctaa tataaatggt aaatcacttc
     1141 agacatatgt atgtgcagga atagaaaatg accatgaatg tataactttt gatgaagtat
     1201 atgaaaaatt gagagcagga ttaaaaattt taataagaga aggaagcgca gcaaaaaatt
     1261 taaagtctat agtaagtggt atgctaaagc ataacttgcc aattgaagaa tttatgtttt
     1321 gcacagatga taagcactta gatgatatag aaaaacaagg tcacataaga tggaatataa
     1381 aatgtgcgat tgatttaggt atggaacctg ttagggctat aaaagttgca acttataatt
     1441 ctgcaaaagc atatgggtta aggaaaattg gagcaatagg tgcgggttat aaggcagata
     1501 ttgttgtatt gaatgattta gacaaaatgg aagtggatag tgtatataaa gatggtaatt
     1561 tggtaaacga agagatgttt tctaattaca attatgagat taaagataaa gaattgctta
     1621 atacagtcaa gtttaaatat attaataaag aaaaaataca gttaaaagta agtgaaaaaa
     1681 attatgtaat ggagattgta ccttatcaaa ttttgacaaa taaggtatat gaatctttac
     1741 catgtgcaga tggatatttt gttccaaata aagagtattc aaaactttgt gttgttgaga
     1801 gacatagaat gactggaaat gtagcagttg ctccactaaa aggatttggc ataaaaaatg
     1861 gagcaattgc tactactgtg gcccatgatt ctcataatat tatagttgca ggggataatg
     1921 atgatgatat tctagtagct ataaattact taaaggaaat acaaggtggt tatgtaattg
     1981 tatcaaatgg aaaagttctt gcacacctaa gtcttcaagt agctggatta attagtactt
     2041 ttacagctga agaagtacaa gaaattactg ataatatgtt ggagatagca agaaaaatgg
     2101 gagttccaga gtatgtagac ccttttatta cactttcttt tatggcactt ccagttgttc
     2161 cacaaataag acttacagat ttaggtctat ttgatgttga ggaatttaaa tttatataga
     2221 taatataaaa gtaataataa atgaggcttt ctcaaaatag ggataagtct ctttttattg
     2281 ttgaaatgat aaaaatatat ttgatttcat acaaaaaaga gcatctcatg aactaaaaat
     2341 tttattttta gatatcctta ttttaattaa tctatcattt aaccttgttt taagcataga
     2401 tatcttaata aaactatttt cagtctattt taacatataa atac
//
LOCUS       DAMJWS010000185         2411 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMJWS010000185 DAMJWS010000000
VERSION     DAMJWS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.191"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            335..2230
                     /locus_tag="PC399_003719"
     CDS             335..2230
                     /locus_tag="PC399_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF2594517.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1052 a          242 c          444 g          673 t
ORIGIN      
        1 tatggagata gatattaatt tagtgatata tatttaagta ataagagtaa agcaaaaatt
       61 agtgcatagt agtatataaa aatggagttt gaatactttt gttagtatat gattgtttag
      121 tagaaaatca ctataaaata ggatttctat ctttgagcat aattatagag agcctaagta
      181 gggggaaggg tttcaagaag atattattag tgtttgtgtt agtaaattat gtaaacacat
      241 aaaatatatt gaaaaataat attatatata atattattta tgtgtgggaa atacgaatat
      301 aaaaaataaa aaataatatt cggaggacat aaatatgtta agcaacaaaa agagaagtat
      361 ggcaatagta atggcaggtg ctacagttat gagtgcagca gcaccaatat tcgcagacaa
      421 taccgtgaca gaaaatgttg ataaaaatta cacagtaagt gcgaaagatt ctgctaaact
      481 aatcgaagag gttagaaaag cattagaagt taagtttgaa gatacaaaag caggagcaaa
      541 tgtaaatgat agagtttacg atataaaagt agacaatgta aacttaacta atgcaactca
      601 attacaaaat aaaataaact ctttaacaga agggcaaagt ttaaaagtta ctattcaaga
      661 taaagggcat caagtgttag gtggaaaagt agttgactat aaaattgaaa actacaaaac
      721 tgctcaagaa atagtagatg cagttaatgc ttacaatgca actttagcag aagattctga
      781 taacaagtta acagcaacta taaaatctac aaatacagtt gaagtaaaaa gagcaaaaga
      841 ttcagcaaat gttattacat taaatgtagg agaccaacat ttagatttct caaaagtgat
      901 aacatcagaa gaaggtactt ttgaaggtta tgaaaagagt tatagtgaca tagattcaaa
      961 agaattacat acgataacag ttaaaaacgc agatttacaa gacatatcag cagaagaatt
     1021 atttgatgga attagattaa ctacattagg aagagaaata gttaataaag ttaaaaatgg
     1081 atatgcttta acatttgaaa atgaagcaat ccttactcaa gaacaagaag atagtgatga
     1141 taaagataaa ccagaaaaat caagctttga tatagtttta agcaaggcaa atgaaaagcc
     1201 agaaacaata agtgtttcaa gtaaaaacca taagttagtt agagacttac acaaagtatt
     1261 aactgatgta aaagatggaa aagaattaaa agtagaagtt ttatctggtg attcaagatt
     1321 tacaacagca gtagaagtta gtaaagagag atttaaagat ggagaagcag aagctataat
     1381 tttagttgga gaagatgcta tagttgatgg attagcatca gcaccacttg cctctcaaaa
     1441 aaatgcacca atattattat ctaaaaaaga ttcactacca tcagaaatag aagctgaaat
     1501 attaagagta cttggaagta atctatcttc taagaaaata tatatagtag gtggagaatc
     1561 taaagtatca aaagaaactg aagaaaaact ttctaaatta ggtgtaagta aagttgagag
     1621 agtttctgga gaagatagat ttgaaacttc tttagaaata gcaaaacaat taaaagatac
     1681 atttaagact gcttttgtag taggtggaaa tggagaagct gatgctatga gtatatcagc
     1741 tagagctgct caatttggtg ctccaataat agttacaggt aacgaattag atgcaaatgc
     1801 tgaaaaatta ttaaaaggaa aagaattaga aatagtgggt ggagaaaatt ctgtatcaaa
     1861 agaagttgaa gacaaattag ttgatataga tttaaataat aaagttgaaa gattagctgg
     1921 agaaaataga aaagatacta atgctaaagt aatcaataaa tactatgcag gtgcaactaa
     1981 agcatatgta gcaaaagatg gttatgtagg tggaaatgga caattagttg atgcacttac
     2041 agcagcacca cttgcagcta gttcaaaggc tccaatagta ttaactacag aagaactttc
     2101 taaatcacaa gaagaagtag ttgagttaag acttaagaat gctactaagt tagtacaaat
     2161 aggtgaagga atagctaaaa acgctattga aaaaatagca gaaaaaataa acttatttac
     2221 taagaactaa taagatatag gaattaatat taaaagttta tttagataat atatgattat
     2281 acaatgcgtt aatatgtaag acatagtgta gatattataa aatatacaaa taacaagata
     2341 aaataataaa aataaggtgt ctcatttaga attagattga gataccttat tttatttttt
     2401 gattataatg t
//
LOCUS       DAMJWS010000186         2376 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMJWS010000186 DAMJWS010000000
VERSION     DAMJWS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.192"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            43..960
                     /locus_tag="PC399_003720"
     CDS             43..960
                     /locus_tag="PC399_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594518.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILATIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            complement(1102..2283)
                     /locus_tag="PC399_003721"
     CDS             complement(1102..2283)
                     /locus_tag="PC399_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2594519.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
BASE COUNT          768 a          403 c          304 g          901 t
ORIGIN      
        1 ttatctatac atttaaatta aaatataaaa ggagtgtatt tagtggaaaa taataaatgt
       61 agagaggact ttagatttac acaagaatat gaggaagatt atccaaatac aaatgaaaga
      121 tactatgaaa attatcaagt agctgataga tactataatt atccaaataa atataaagaa
      181 cctaaaataa aacaatgttg ttgtaaaaaa agtatgagag aagcattaga acttctaaga
      241 tatgatgctc taagaccttt tgtaaacttt aatcaatttg cttttatctc agatttcttt
      301 atagtaggtg ctaatttggt aggtatagat ctttcagctc ctccaaaaga taatttatct
      361 ggacttgatg gtacttttga aagattttct gcttgtaact gtgatttaat agatatagct
      421 ggtagagtat cttatcctat tccagtccct ttaactcttg agggattaat taatactata
      481 ggaactatac caggagtagc tgaattaatt gcacttattg atgcagttat tcctcctacg
      541 atagaccttg gggctatatt agatgcaata cttgctacta taattgattt tatacttgct
      601 gcatctactc cattagcaaa cgtagattta gcatcattgt gtaatcttaa agctgttgca
      661 tttgatatta cacctgcaga ttatgaagat ttcatagcat ctttaggtta ctatcttgat
      721 aaaaaacatt acaaagaatg taattgcaac tgcgattgtg atgattgctg ttgtaataaa
      781 ggtatcctag ataatcttta tatgtcaaat ataaataatc aagttactgt agtagctggt
      841 agtttggttc taactggtgt tgaagttcta ggtaagaaaa atgatgttat agtacttgga
      901 aattctaatg attcaagaat atactttgta tgtgtagatt ctatagatta tattgcataa
      961 gaatgatttt aaagagttat atctaaatat aaaatagaat ctattttcta aagtataaaa
     1021 gatttaatta ttactaataa aaaagagtgt ctataattat ttaaaaattt ctttagacac
     1081 tcttttttat ttttatacta tttatctaac ttttttacca agtttaaata ctgctaacac
     1141 atctgcaagt acgtctatat tctctactgg atttccattt acaactataa catctgcttt
     1201 ctttcctttt tctagagttc cataattttt atcaattctt aaaagttcag atgctgtttt
     1261 tgtagcagca actattatat ccatattatc cataccataa tctgacatta gtttcatttc
     1321 ataatatgtt tttccatgtt cattataaga agttcctgca tcagaaccaa ctgcaatttt
     1381 aattcctttt ttatatgctt ttaggaatga ctctttatgt ggaatggcaa caatctcaca
     1441 ctttctaaga gtagcttcag ttattccact ttcttttcct gcatttagaa taaaatatgg
     1501 tgcagccaca gttggaacta agtaagttcc tctttcaagc atcaaatcaa ttatttcatc
     1561 atctaaataa acaccatgtt ctatagaagt aattcctgca agcactgcat tttttatacc
     1621 agttcttcct tgtgcatgtg tggcagttat tttacctgct tttatagctt catctacagc
     1681 agccttcatt tcctccaaag ttaattgtgg tgaccctggc tcaactccat cagttgttac
     1741 acctccagtt gccattatct ttacacaatc tactccattt tttaaaactg ttctggctga
     1801 ttttctacat tcatccactc catcactttc aataccaaaa taatatccat gccctcctgt
     1861 catacatata actggacctg aagtaactat accaggacca tctatagacc ctttgtctat
     1921 aagattttta agttgtacat ccacatattt gggacaacct aagcttctaa tatatgttac
     1981 tcctgaatcc aaataagcat caagttgttt tatcgtattt actacaagct ctacatctga
     2041 tacattatca taatatatct ttggatttcc aactggctcc attgtcaagt gaacatgaca
     2101 attcataatg cctggcataa cagttttatc taagtaatcg tacacatcat atccatttaa
     2161 atcatctctt atatcttcaa tttcaactat agtatctcca tcaataatta tacccttatt
     2221 ttgcataata ctatttccgg ttccatcaat taaattccct gctttaatat attttttgtt
     2281 catcttaaat ttctccccct tttaagtaca atgtattatt tatttattat ttctaatatt
     2341 ctaataatta attttaccac tataagcatt ctattt
//
LOCUS       DAMJWS010000187         2319 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMJWS010000187 DAMJWS010000000
VERSION     DAMJWS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.193"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(121..1413)
                     /gene="brnQ"
                     /locus_tag="PC399_003722"
     CDS             complement(121..1413)
                     /gene="brnQ"
                     /locus_tag="PC399_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2594520.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIISPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          767 a          372 c          255 g          925 t
ORIGIN      
        1 tatcaaatag gattaataat gttcttttat ctacaataat ttaataattc ttttaaatat
       61 aaaaaggaat tgcctatata atttaaaggc aattccataa ttatcaatta gtttttatta
      121 ttatgcagtt ttactgcttt tagatggaat aaagtttcct attattcctg ctattacaac
      181 tggaactatc cagttaaatc ctaatgaagc aaatggtaag ctattaacta gaggaatttt
      241 tacaccaaaa cttgttgcaa ctgttaatac acttactaat aatgccatgt atgcagcaca
      301 tttgaacacg ttgtcatttt taattttatt actaaataat gctagtataa ctagagttat
      361 agttgctgga tataccatac ttagtatagg agaagagaat tgtattattg tacttactcc
      421 aaagtttgat atgattgcac taaatacaca aactactata actatatgct catactttaa
      481 ttttccatct gtaagtgttg agaaatattg accagctgct gaagaaagac ctatagctgt
      541 tgttaaacat gctaatgcaa ctataatagc caataaaatt ttacctgtag ttccaagtaa
      601 agctgctgtt attgcaacta ttaaagctgt ttgttgaaca tcttgtccat attgttgaga
      661 cacagttgct ccaagataac ataatccacc ataaactaat gccattccta ttactgcaat
      721 tacacctgca cttattgtgg ctttcatctt ttctttctca cctgtataac ctttagctat
      781 tactgacatg attataattg atgcaaatac actagctgcc aatgcatcca ttgtttgata
      841 accttgagtt ataccttctg caaatacatt ttgtattaaa gaactgtctc ttatttctcc
      901 taatggagat atgatacctt taactattaa tactgctaaa gctataagta aagctggagt
      961 taatattgac ccaactatat ctacaacttt tgatggtctt attgttaaaa ccaatactag
     1021 agcaaagaat actatagaga atattactgg gcttattcca gaacctattg taggtaatat
     1081 acccatttca taagttgttg ctgctgttct aggtattgct aaaagtggcc ctatacagat
     1141 taccattgca catcctaatg caatacttaa tcctttgcct gctcttgaga acatcttcga
     1201 tgtgtctcca ttgaacttag ttgctgctat aactgctaat aatgccaaac caccatctgc
     1261 aaaaaggaat cctatgaaag ctattagcca actacttcca gataaaactc ctagatatgg
     1321 agggaagatt aagttccctg caccaaaaaa cattgcaaat agtgcaaatc caattacaat
     1381 tacatcttta aaattgattt gtttctcacc catttaaagc acccccattt gaattttact
     1441 accttttact aaaaaataag tccaatctct tcattaaatg aaaacctttt gacgagattt
     1501 tatttaatta tacatttttg acttaaattt gtcaattatg tttcataatt tataaaatct
     1561 tcacaatatt taatacaact ttcttaatta aacaaaatca acctgtatac tttaaacaca
     1621 gttggttttt tgttttttct ctaatttttc ttgataaaat taattattct gaataattaa
     1681 aatattaaga ttttttagat tattcgattt gttattaagt gtattgcttt atatactgta
     1741 tatatcactc tcctttttta cattcatata atctttttaa ttcaatttaa attttaaata
     1801 ttttgtgttt attatataat ctgaaaaaaa agcagtcaat agcattttta atttttttaa
     1861 ttttcaaaat tatgattctt aaaaaatcgt tttccttttg tcatagcacc tatttacgtt
     1921 aattcttgta gtattttgta tttattttgc taaattgaca gttaacttta ttcgtttttt
     1981 attctattta tgtagatttg cctcttgttt tttagtctta aaatcatttt ttctgaaaat
     2041 tccttattat gatttatcaa cttatttttc ttcatatcaa ttactttcta acttccaact
     2101 tacttaaact ttgcagttgc tagtattcac gtttacatac ttatctgtca taattaacta
     2161 aaaattctta atatatctct caatgaaaac aatttaccca tttacttaat acatttatat
     2221 aatcatatgt cttaaatttt gttttattat tttattattg tcacaatttt atttatatta
     2281 tatttttgta aaaatcattt aatatgtata cttaaattt
//
LOCUS       DAMJWS010000188         2294 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMJWS010000188 DAMJWS010000000
VERSION     DAMJWS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.194"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            142..282
                     /locus_tag="PC399_003723"
     CDS             142..282
                     /locus_tag="PC399_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594521.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(279..650)
                     /locus_tag="PC399_003724"
     CDS             complement(279..650)
                     /locus_tag="PC399_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594522.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1199..1456
                     /locus_tag="PC399_003725"
     CDS             1199..1456
                     /locus_tag="PC399_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594523.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1566..2246)
                     /locus_tag="PC399_003726"
     CDS             complement(1566..2246)
                     /locus_tag="PC399_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2594524.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          924 a          259 c          265 g          846 t
ORIGIN      
        1 ttaagataat tatctaggga aattttggat aatcaaaata tattttataa aatcaaaata
       61 caattacata aactttatta aaaatttgtt gggaatttct gactaacatt aagaacgtaa
      121 gtttgttggg ggatggtata tgtggaaaag tcaaacacta ctgttacaaa aaaagaaaaa
      181 attgaagata taggaatgaa attagaacat ttattgagtg aagatattac tttttatgaa
      241 ttattacttg agattttgta taaagaaaaa gaagagaatt aattctcttc tttaaggaat
      301 ctttttaaat aggcattatc aatcattttt tctatagctt ctctatcttc tttttcaaga
      361 gaatatattt tttctaagag cttttttgtt ttttcactta ctgataagtt ctctatgatt
      421 aatttatctt ctttgcttat ttttgtatta tcatttacgc ctaataaata atctgttgat
      481 gtttctaatg cttttgctaa tctaactatt atttcacttt taggttctct taaattattt
      541 tcataacgtg acaagctagc ctcagttata ccgactttag tagcaagttc tttttgattt
      601 atatctaatt cttttcgtag atttgctatt ctatctccta aactttccat atgtacatca
      661 ctccttaccg aaattataag ttattttgat aaaaagtaaa ataaaattac caaaaatata
      721 aaaaaaaaag gttaaaataa taaaatatat tgaaaattac catttgtata tgtataatat
      781 gaattataca aatggtaatt tgatgagagg tggtatgttg aaataagaaa ctgaattaat
      841 taataaatac atagaaagtt ttgaaaacct ttaattgaaa tataaacaat atgtacaaaa
      901 ttttttgcaa gtaaaattac caaatgtata aaataaatca gtagcataga taattttatg
      961 ctttaataca ttagattatt taatttagtt ataatggtca aaaatgcaat ctacaaataa
     1021 aacaaagtat tatcaaaaaa gtattcttta agaaaatttt ttattagaga tgtaattgat
     1081 ataaataatc gtattaaaaa ataaaatact aagtttaaaa atttaaataa tctataaatc
     1141 aattataaaa tattatttct tagaaattaa aatgaattaa gtaaatggag gaattgatat
     1201 gataataatt agaagtcaag ataaaactaa tttaatgcag ataaaccaaa ttaaaataga
     1261 tggtagtcaa gtttatgcag tatttgaaag taaaattgat actgtaaaaa taggagatta
     1321 tgaaaataat acaagagcta tacaagtgtt ggacaatata caaaacttca ttgaaaatgg
     1381 aacaaaatat gattatataa catcaaataa agtcagatac aatgttaata aaatatttca
     1441 gatgccagct aaatagtgag tgtgcatttt aatttaataa ctaaagccca ctttcttttg
     1501 tggtatgaaa gagggcttta catatttgta aatatcgaag tacacttgtt ttatcaataa
     1561 attatttaat aaggtataat ggaccaatta aacaataaaa attctcctaa aacagcaagt
     1621 attaaatata taatagcttc atttttgtaa agagaggtaa ttttttcgct tatgtcatca
     1681 tcaattgtaa tagcaatttc atcttgaata atatttttat atatagatgg gaaaatatct
     1741 tttttcttta taagattaaa taaattattt acacctaccc atataaaata tcctaaaaca
     1801 gcacctaatg tgttcatcat taaatcatca atatctgtag ctcttaagtt aaacaattga
     1861 caaagttcaa ttgctaatga aaaacctaat ccagttaatg aaacattgat aatatttcta
     1921 aaatttttcc aaattaaagg tagtaaaaat cctaaaggca taaacataat tatatttaaa
     1981 atatatgtta agatgccaac tgagtcaaaa ggtattagat ttatttcatc aattctaaaa
     2041 agcccactat accttcctat atcccatatg ctaccaattc cagctttact aagtgctaga
     2101 tagatatata ataaaaaaat atatacccat actaaatgta tcattacatt cttttcaact
     2161 ttcaaatttt ttcgttttat aacgactatt tggtatatta agcatggtaa tataacgcaa
     2221 ataaatgaat aaaatgtcca tatcatttta ttttgtcccc tttcaaattt atgtttttag
     2281 ttttataagt acta
//
LOCUS       DAMJWS010000189         1986 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMJWS010000189 DAMJWS010000000
VERSION     DAMJWS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.195"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            84..200
                     /gene="rrf"
                     /locus_tag="PC399_003727"
     rRNA            84..200
                     /gene="rrf"
                     /locus_tag="PC399_003727"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..281
                     /locus_tag="PC399_003728"
     tRNA            207..281
                     /locus_tag="PC399_003728"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:239..241,aa:Asn,seq:gtt)
     gene            286..360
                     /locus_tag="PC399_003729"
     tRNA            286..360
                     /locus_tag="PC399_003729"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:319..321,aa:Glu,seq:ttc)
     gene            366..441
                     /locus_tag="PC399_003730"
     tRNA            366..441
                     /locus_tag="PC399_003730"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Val,seq:tac)
     gene            447..523
                     /locus_tag="PC399_003731"
     tRNA            447..523
                     /locus_tag="PC399_003731"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:481..483,aa:Asp,seq:gtc)
     gene            534..608
                     /locus_tag="PC399_003732"
     tRNA            534..608
                     /locus_tag="PC399_003732"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:566..568,aa:Thr,seq:tgt)
     gene            623..707
                     /locus_tag="PC399_003733"
     tRNA            623..707
                     /locus_tag="PC399_003733"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Tyr,seq:gta)
     gene            717..790
                     /locus_tag="PC399_003734"
     tRNA            717..790
                     /locus_tag="PC399_003734"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Gly,seq:tcc)
     gene            801..877
                     /locus_tag="PC399_003735"
     tRNA            801..877
                     /locus_tag="PC399_003735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Arg,seq:tct)
     gene            887..962
                     /locus_tag="PC399_003736"
     tRNA            887..962
                     /locus_tag="PC399_003736"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Gln,seq:ttg)
     gene            974..1049
                     /locus_tag="PC399_003737"
     tRNA            974..1049
                     /locus_tag="PC399_003737"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1007..1009,aa:Lys,seq:ttt)
     gene            1052..1140
                     /locus_tag="PC399_003738"
     tRNA            1052..1140
                     /locus_tag="PC399_003738"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1086..1088,aa:Ser,seq:tga)
     gene            1158..1248
                     /locus_tag="PC399_003739"
     tRNA            1158..1248
                     /locus_tag="PC399_003739"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1192..1194,aa:Ser,seq:gct)
     gene            1257..1333
                     /locus_tag="PC399_003740"
     tRNA            1257..1333
                     /locus_tag="PC399_003740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1291..1293,aa:Pro,seq:tgg)
     gene            1419..1494
                     /locus_tag="PC399_003741"
     tRNA            1419..1494
                     /locus_tag="PC399_003741"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1452..1454,aa:Trp,seq:cca)
     gene            1555..1631
                     /locus_tag="PC399_003742"
     tRNA            1555..1631
                     /locus_tag="PC399_003742"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Pro,seq:tgg)
     gene            1638..1714
                     /locus_tag="PC399_003743"
     tRNA            1638..1714
                     /locus_tag="PC399_003743"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1672..1674,aa:Ile,seq:gat)
     gene            1718..1793
                     /locus_tag="PC399_003744"
     tRNA            1718..1793
                     /locus_tag="PC399_003744"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1751..1753,aa:Phe,seq:gaa)
     gene            1801..1877
                     /locus_tag="PC399_003745"
     tRNA            1801..1877
                     /locus_tag="PC399_003745"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1835..1837,aa:Met,seq:cat)
BASE COUNT          517 a          452 c          531 g          486 t
ORIGIN      
        1 taactctaaa aaaaaataaa aaaagtattg acaaaaatag taaaaaatgt taatattata
       61 cttgtcctag caacaagaaa aaatgtggtt ataatagcgg aggagataca cctgtttcca
      121 ttccgaacac agaagttaag ttctctagcg ctgatggtac ttggtgggaa actgcctggg
      181 agagtaggac atagccacgt gaatgttcca aggtagctca acggtggagc aaccggctgt
      241 taaccggtag gctgtgggtt cgagccccac ccttggagcc attatggctc gttggtcaag
      301 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      361 tttgtgggat catagctcag ctgggagagc acctgcctta caagcagggg gtcacaggtt
      421 cgagccctgt tggtcccacc atttgcggcc tggtagttca gttggttaga atgccagcct
      481 gtcacgctgg aggtcgaggg ttcgagcccc ttccaggtcg ccaaaataaa aatgctggtg
      541 tggctcaacg gtagagcagc tgacttgtaa tcagcaggtt gtaggttcga ttcctatcac
      601 cagctccatg taaaactaat acggaggatt tcccgagcgg ccaaaggggg cagactgtaa
      661 atctgttgtc atcgacttcg gtggttcgaa tccaccatcc tccaccaatt aaatatgcgg
      721 gtgtagctca atggtagagt tctggccttc caagccagct gtgagggttc gatccccttc
      781 acccgctcca aagaaagcgt gggtgcatag ctcagctgga tagagcaacg cccttctaag
      841 gcgtgtgtcc ggggttcgaa tccctgtgcg ctcaccacat atatattggg gattagccaa
      901 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      961 caaataaaaa tatggtctat tagctcagtc ggtagagcac ctgactttta atcagggtgt
     1021 cccgcgttcg agtcgcggat agatcaccaa tggagaggtg tccgagtggt ttaaggagct
     1081 ggtcttgaaa accagtgact tcgaaagggg ccgtgggttc gaatcccacc ctctccgcca
     1141 aaaaaatgac tgaatttgga gaagtactca agtggctcaa gaggatcccc tgctaaggga
     1201 ttaggctggg taaccggtgc gagggttcga atccctcctt ctccgccatt caaaaacgag
     1261 gtgtagcgca gtttggtagc gcacatggtt tgggaccatg gggccggggg ttcgagtccc
     1321 ttcacctcga ccaacttaag cttttagttc agttggttag agtgcccaac tcatggctag
     1381 taattctagg gtttgagtcc atgaaggtta ttcttcatag gggtgtagct cagttggtag
     1441 agcgtcggtc tccaaaaccg tgcgccgggg gttcgagtcc ctccacccct gccaaataaa
     1501 aaggacttga aaaaagttgg attttagagt agtataagat agtaaataag atttcgaggt
     1561 gtagcgcagt ttggtagcgc acatggtttg ggaccatggg gccgggggtt cgagtccctt
     1621 cacctcgacc aagaaaaggg cctatagctc aggtggttag agcgcacgcc tgataagcgt
     1681 gaggtcgctg gttcgagtcc agctaggccc accatttgcc cagatagctc agtcggtaga
     1741 gcaggggact gaaaatcccc gtgtcggtgg ttcgattccg cctctgggca ccaggaatac
     1801 ggcggtatag cttagttggc tagagcgttc ggttcatacc cgaaaggtca taggttcgac
     1861 tcctattacc gctaccatct aactatatca ataaagaact ttgaaaatta aacagtaggt
     1921 taatttataa acaagaaaca aaccataaag ccagatattt tgataacaat agtatctgag
     1981 cctgat
//
LOCUS       DAMJWS010000190         1941 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMJWS010000190 DAMJWS010000000
VERSION     DAMJWS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.196"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            79..1821
                     /locus_tag="PC399_003746"
     CDS             79..1821
                     /locus_tag="PC399_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HDF2594525.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
BASE COUNT          764 a          229 c          269 g          679 t
ORIGIN      
        1 taaattttct taacatgcat tttttatatt taatatagta aaataattaa taactatatc
       61 catgattgga ggatttttat ggttaaatcc aagaagaaaa tatttgtttt tctaatttta
      121 attgcagttt ttattatatt tgcatcatac ataagaaatt atactaatga tacaaaaaaa
      181 gattctgtta gaggaactat tccttttgaa aaaatggaat ataagcgtcc taatatacag
      241 tcaatttgcg aaaacattaa caagtgtaac gacaaacttt taacagctaa aacagctaag
      301 gaacaattaa atttatttaa tgaagttgat aaaatttatc aggattttta cagtacactt
      361 actatagcaa aaattagaaa taacatagat tcatcagacg aattttactc taaggaatat
      421 aagtatctta tgagtaaatc agtagatgtt gacatgtcat acaaagactt tcaagataga
      481 tttgtaactt ctaaattctc taaagaatta gaaaaagaac ttggaaagga tacatttaat
      541 tatctaaaga acattggaaa acttaattct aaagaagttg aaggtttgct taaaaaagag
      601 caagacctag ttgtaaaata tgaagattta ttagctaaat ctacagtatc aatagatggt
      661 atagaggtag actttgaaga agccttgtca agaccaaatc ttagccctga agaatatgtg
      721 aaaatttatt cagattactt aaaaaaatac aatcctatat ttggtaacat attcttagaa
      781 cttatacaga caagaactga aatagcaagt aagcaaggat ttaagaatta tatagattat
      841 gcctatatga acctaaacaa agactattct caagaagaag ctaaaaaatt tagacaagat
      901 gtaaaagatt acatagttcc attatataga gagatttcat ctaaaccatc tgattcttct
      961 atttatatta aagtatataa aaatcgctca tttaggaagt ttgataaagt acttgaagat
     1021 atatctccaa aactaaaaga gagcttcgac tatatgaaaa aatatgactt gtacgattat
     1081 tcttctggaa aaaacaaatc acctggagga tatactactt atataaataa atataaagca
     1141 cctttcttgt ttaatacatg ggacaattct tttttaggtg ttactagttt tgctcatgag
     1201 tttggtcatt tttataatta ctacaacagt attaacttaa ataataagat gcaaccaagt
     1261 atagatgtat gtgaagttca ttcaacatct ttagaaattc tattttataa atattttgat
     1321 gatttttttg gtaaacaatc tgaagctata aaaaaagaac acttatcgat tgttttaaat
     1381 actattattg atgcttgtct ttatgatgaa tttcaagaaa ttatatacaa gaatccatat
     1441 atgagtctaa atgatatcaa taaattgttt tttgatttag aggaagaata tggagtttcc
     1501 aataatatac ttagagaaaa aaatgctcct ttttggatac ttgtatctca taacttccaa
     1561 gttccttttt attacttgag ttatggtctt gcatctgatg tatcacttca gatatggcaa
     1621 ttatcacaag aagattacag aaaagctgtt gatgtatata tggatttttt aaatcaaaat
     1681 actgatgctg gatttaaaga tgtggtagaa aaggtaaatt tacaattacc tttccaaaat
     1741 ggaaatttag aggaaataag ctctacttta tatgattatt ttggtataga taatccactt
     1801 gaactaaaaa atgcttctta atttctaata ctttatactc actcttaaga tttagtatta
     1861 gattgaaata agtgatattt tttcacacac aattaatgtt taataaaatt atatggtaaa
     1921 gtatcagaaa tttttaagac t
//
LOCUS       DAMJWS010000191         1924 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMJWS010000191 DAMJWS010000000
VERSION     DAMJWS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.197"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            164..448
                     /locus_tag="PC399_003747"
     CDS             164..448
                     /locus_tag="PC399_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594526.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(605..778)
                     /locus_tag="PC399_003748"
     CDS             complement(605..778)
                     /locus_tag="PC399_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HDF2594527.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1219..1434)
                     /locus_tag="PC399_003749"
     CDS             complement(1219..1434)
                     /locus_tag="PC399_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594528.1"
                     /translation="MAIVVNLDVMMAKRKISLKELAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1445..1912)
                     /locus_tag="PC399_003750"
     CDS             complement(1445..1912)
                     /locus_tag="PC399_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2594529.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          785 a          203 c          234 g          702 t
ORIGIN      
        1 tatattttac taatatacat ttaaaaatta caatttaaag cattaaaaaa taaatttgta
       61 ttttgtttta tataaagtaa tttgaaaaat atggaaaaat tgattagtac attattaatt
      121 agggtataaa atggtgtaaa tagaatgata aggggtggat gaaatgcttg tatataataa
      181 aagtttttat cctaatgaca tatttccaag attagatttt tcaaaaataa aaaaacagtt
      241 aaaattgata gataatgacc tgtcagattt tggaagcata tgtataatag aaaaagaaca
      301 ttatacgata agtgtaaaca gtataggtga aataaatgtg tactatgatt tagagtacga
      361 aaataaggtg tatagaatag tttatgagat tgaaaagtta tttaaatctc aagttggaag
      421 gtttagcata tctacataca gaaattgata attaaaaaag tagaaattaa aaaacttaat
      481 actaaagata tagataaata tatgaggtaa cagctagaga gagttgttac cctttttaaa
      541 tactaagtat atattattct tattaatttt ttgtaaattc actaaaagac ctttttatag
      601 aatattattt tcttttttta ttaaataggt aaatcagtaa aaatgcaatt ccagttccaa
      661 ttattattcc aatgatattt tctataatta atttttttaa tataaaagta aataataaaa
      721 atgctataca taaacctaaa atatatcctt taaaataggt tgaaagtttt cttttcatat
      781 aatcaccaac ttgtatcaga tttagtttac ttaattagag tataacagtg ttgtaacttc
      841 aaagagtatc taaaagatta caaatgttag ataaaatcat caataatgta atttataaca
      901 catagctata agctatgcaa taatataaat ggaggtataa gtaaatgaaa aagttccatg
      961 ttttgctaac agtttaaata aatattatgt agaaaagaat gtatatagga atctgtggta
     1021 aaattatcaa tttaaaagta ttatttagtg tgatacagtt cttataggta atggataaca
     1081 aatagataaa ggaacagttt ctttcttaga acatattttg ttatgacttt aaacaaagtt
     1141 aatattaatg tcaataatat agtaaaagaa taaaaatcat atagtataat aaaaaagagt
     1201 gtttctaaac actctttttt aatcattttc atcttctaca tattcaagta tatcacctgg
     1261 ctgacagtct aaagctttgc aaatttcgtt tagagtggta aatctaatag cttttgcttt
     1321 tccagttttt aaaattgata aatttgcatt tgtaatatct attttttctg ccaattcttt
     1381 aagtgatatt tttctttttg ccatcattac atctaggtta actactattg ccataacatc
     1441 acctctatat cgttaaatca ttatcttctt ttatttttat agctttatca aatgtttctg
     1501 caattacaaa gcacattagt gcaaatataa aagagataac cataagtggt gagataccgc
     1561 cactatcagt tccaaaaatt cttatattat tagtatttaa attataacca ttgatacatt
     1621 caattatagt gtttatcaat aaacaaaatc ccattacttt aaaccttttt acattttcaa
     1681 gtacgaatgg ggaatactta gtactattaa ttataaatat caagaataag atgactataa
     1741 agtagtcaga aaaatatagt attcttgcaa ttaaagcaat tactttgtca gaactaaaat
     1801 catataaaaa cataggtatc atacataaga atattatacc agcaactcct aaaagtgata
     1861 atagaaaata tagaaatact gataatatat tactaaaaaa ttttgtaatc ataaaatatc
     1921 atct
//
LOCUS       DAMJWS010000192         1880 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMJWS010000192 DAMJWS010000000
VERSION     DAMJWS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.198"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(813..1130)
                     /locus_tag="PC399_003751"
     CDS             complement(813..1130)
                     /locus_tag="PC399_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594530.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
     gene            complement(1440..1796)
                     /locus_tag="PC399_003752"
                     /pseudo
     CDS             complement(1440..1796)
                     /locus_tag="PC399_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          231 c          185 g          776 t
ORIGIN      
        1 atattgggat gtttttaaat atttttggaa attatatata ttataacaat tatagcaaat
       61 ttttagggta taatttttaa aattatattc tactatcaaa agatatgaat aaatgattgt
      121 atatattata tcaattttta tatttcatag tccatttttt catcacattt tcgcttgtca
      181 agaataatat ctttaagagt gaagcgtttg atgtaaatct ggactttgtt tttgagacat
      241 ttttgaaata tttattaaat ctttcgatta tacttgtaat atatattata gatattactt
      301 attttagtaa aactaaagga tgatgctaca acattccagt tatttcccaa aattttaatt
      361 tctgaagtat actgtttaca ttatttttaa tctattttat ctaaagtcga taaggcttct
      421 tattcattaa ctgacctata tacagattta aaattactta ttaattcttt tgagtccttg
      481 tatgaaacat atttaaagta tttcttatct aatgtattaa acatttttgt attacagaat
      541 gaggataact tgtagctatt gtctctttga aataagtcat ctgtgtttac tcctaaaact
      601 acttatatct gtagatttta taagttctcc atcttatata atttctttga ttaattgttt
      661 tcttaaatta ttcataaaaa aatctccgtt acgatatttg attatattta aatcattgct
      721 ataaagaaga cttttattaa ctttgaaaaa caaattattt taattgctgg tgattagttg
      781 ctaccttctt ttgatattaa ttctattaga ttttatatag ttatatatct ctttctaact
      841 aagtaatata atattactgt tatagtagct actaatatta ttgctattat tgtaatatct
      901 aatctttttg taaataatcc tattgctata tattcaaaac ctgttacata tcgataaagt
      961 acttctagtt taatagttct ttttctatct atatttccat attttgattc taatttatta
     1021 aaatattttt cattacaaaa accaatttta atttcaatta aagatattat tgtagttaat
     1081 attataattc ccaatcccat aagtataaga taaatattcc taaaattcat agaagatacc
     1141 tcctttataa tataatcatc aactcatgtt gtattaattt tcctaatcaa agtataacag
     1201 taacacaagt ataaagagta tctaaaatat tacaaatatt aaatgagaaa aagatggtaa
     1261 gttaacccca ccatcttatt ttttatctac taatgaatga tgctacaaaa gttagtataa
     1321 aaaatgaaac aaacaatata atgaacttgt gtaatttact tagttggaat gctttattac
     1381 aaaataataa aattaatgct gaaacaattg caaaaagtag atattttata atcattttat
     1441 cacctattga tttctacaga ttgtaagtag tatttggtta cacaagcacc tcctttatta
     1501 taacgtattt tttaaattta aagatttttc cagatgttat atttgactca aggtacattg
     1561 taattaatct aactataagt ggcaagtact gtcctatacc aggaatagca ccagccaaaa
     1621 gtcctgaaat ccctatacca gcatcagtta aagttaatgc aactccttta gaaagatata
     1681 aattaaaacc tattctagcc caaactattt tatttatttc agcttttcta taatataatt
     1741 caagtagtgt caatatcgtt cttcttaagg tttttaatta gcttatatat tgttattgta
     1801 taatataatt attaaaaatt aactatatta aatattaata aaaaatatag taaaacttaa
     1861 taatacatac aataaaactt
//
LOCUS       DAMJWS010000193         1839 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMJWS010000193 DAMJWS010000000
VERSION     DAMJWS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.199"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            114..308
                     /locus_tag="PC399_003753"
     CDS             114..308
                     /locus_tag="PC399_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF2594531.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(410..919)
                     /gene="vanZ1"
                     /locus_tag="PC399_003754"
     CDS             complement(410..919)
                     /gene="vanZ1"
                     /locus_tag="PC399_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF2594532.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1239..1628)
                     /locus_tag="PC399_003755"
     CDS             complement(1239..1628)
                     /locus_tag="PC399_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2594533.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEGDIDD"
BASE COUNT          681 a          256 c          190 g          712 t
ORIGIN      
        1 tgcattataa taaaaagtaa atctaaattt gatacagttt taacaatcat tattttcaga
       61 aaagtgatac tattgaatat attaagaata attagagatg gaggaactaa gatatgagcg
      121 taattgtaat gtgtagtcgt tgtggaaaag aaatagataa atatagaaat aaatatataa
      181 cttattatga gaaaaatacc aatgcagaaa ttaaagtttg tataaattgt gcgttagatt
      241 tattagaaaa aacaagagaa aatcaagaat tgaacataca agagtttcaa gatacaataa
      301 ttctttaata atttaggaaa gcacctgtat aggtgctttt tatgtgtaaa taattttata
      361 tgtaactgta tcttacgttc tattttaagc taaacatata tacttatttc tataagtttg
      421 caaaaataac taatcctaaa aataatacaa caataattgt cacaatcaat gccaatacat
      481 tgataatttt tatggtatta ttcttaaata ttttcgataa tagcataaaa acagctattc
      541 ctatgattcc tcctagtgtg tttcctatca aatccgttat atcacttgca cctagtgcaa
      601 atatatattg aagtgtttca aatgccaaac tagttataaa aataggtata acttttttta
      661 taaagctcca ttcttctttt agcatacaca catatatccc aaatggtaca aatatagcca
      721 catttaatat aatctctgat atatctactc tattattaat tataagtgac ccagcaaaag
      781 gtactaagtt tatgcttcta agattcatgg tttcaaggga tgaaatatca aattgcaact
      841 tgaatagtat tatccatgtt aaaataatta tgtaaactat aaataatcct tttgtaatat
      901 tatgttttct agatttcatt tattacctcc ttactatatc attgtattat tttattaata
      961 caatgatata gtaaggtaat ttatctgtca actatttctt ctaaaattat attcctttta
     1021 taatttcaat atctttatca tatttacatg atttaagcta ttgtagtatt tcatatatct
     1081 ttaaatttta attggtatta ttctctatat tttttactat caaacactaa ggaaataatc
     1141 acttttcttt atataatgtt ctaccatcac ataaaactaa cattaaatca aaacgctact
     1201 ctcaattgtc tcaaactaaa aagatactga taactttatt agtcatcaat atctccttct
     1261 ttcaaatttt taaagtattc ttctaacttg tccaagctct tactatctaa aacttcatca
     1321 tcatgtaatg ctgaaataaa actcttaaat gaattgttat gcataaaact aaaaaaactc
     1381 tttgtctcaa cttttaagta atctttctct tttatagcta tagtataatg cgtacatcta
     1441 tctaccttat cagtagttaa aaagccctta tctactaaat tttttaaaaa tgtcaatgtt
     1501 gtgctctttt tccaatcgta tttcaacttc atgtcttcta tgatatctct tgaggctaat
     1561 acatcatcta ctccccaaat atacttcatt agttttagtt ctgcatgtgg taatttatta
     1621 atcttcatta gtttacacct cttaccaatt tatgtataac taaactttat aacaataagt
     1681 gtatataatc aattagattg gaataaatat aaattccaat ttattctata taaaaataat
     1741 taataaaaaa gaagtactct actttagaga gtacttttgg attatatatt caaaaattta
     1801 tctaacatga ttagagtatg ttttatgctt tagtgtgat
//
LOCUS       DAMJWS010000194         1838 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMJWS010000194 DAMJWS010000000
VERSION     DAMJWS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.200"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(299..1627)
                     /locus_tag="PC399_003756"
     CDS             complement(299..1627)
                     /locus_tag="PC399_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2594534.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          673 a          332 c          209 g          624 t
ORIGIN      
        1 aaagatttaa ttttcataaa agctatgtat aatatttgtt gtacagatac cacttagttt
       61 gataaaaaac actttaaaat aaagtatttt aaaataaata attttttaga tgtttaattt
      121 tataatcaaa aaactaaaaa taaaatgaat accttaaaat aaacttttta gttcatggaa
      181 cgctcttttt ttgtaaaatt aaacatatct ttaccatttt aacaataaaa aagagactta
      241 tctctatttg agacagtctc ttttatttta attatgaagc atttgtaaac tacttggttt
      301 atctttttac tcctctagtc atataagcat acactggtaa gcctatagct gttattacaa
      361 gacctcctat agatatcata gtagttttca tacctgcaaa acacaattga ttaactacaa
      421 caaatatacc acttgctata gctattatag gaacaatagg atataatgga actttatact
      481 ctcttggtat atttggatga gtctttctaa gtttcataac ccctataaaa gttaatacat
      541 agaatatcca agtagcaaat acagctaagt cagttaataa attaaattga cctgacaaag
      601 catatataga tgcaataaca gatactaatg ctattgcatt tgctggaact tgagcagaat
      661 ttaatttaga aaatatacca tactttggta aagtcttatc tgtagctaaa gtatatgcca
      721 cccttgaacc agttagtaaa aatccattta atgctccaaa cactgaaatt aatataccta
      781 cagatattat ttttccaccc attgaaccaa atataacttc agcaactgct gatgcaggag
      841 aagcataatt tgctaattca ttagctggta atacccaaag atacgctaaa tttattataa
      901 aataaacagc cataacaact gacaatccac ctattatagc ttttggaagg tcttttccag
      961 gattcttcat ttctccagct aaagtaccaa cattcatcca tccatcaaaa gcaaataata
     1021 tagcaattaa aacttgacct agtacactac ccaggcttaa tccttcacca accataggtg
     1081 taagtattgg attatttcca ccacctttaa taaatccaac aaccattatt aaaataagtg
     1141 gtattaactt acaaattgta gaaacagttt gtatgactcc tccagttttt gaacctaaca
     1201 tattaagccc tgcaactatt actataattc caattgctat tggaagtaaa agtgatggac
     1261 taccaattag tgctgatgct tgttctccaa atataacacc taatgctgcc atcatccctg
     1321 gataaaataa tacaatttgc atccatccag ttaaaaaacc taatttttct ccataaattt
     1381 cttttatata taccatcatt ccaccagttt ttggtatcgc aactgatacc tctgcagctg
     1441 taagaccagc tgttatagtt atgattccag ctattaacca tgccaatata ccaagacctg
     1501 gagctcctcc agttaatgta tagactgctt gtggtttaaa aaatacgccc gctcctataa
     1561 cggaaccaac aacagttgat aatgctgctg atacgccaag agtctttttc agttcattac
     1621 ttcccatttt tttcctcctc attaatccta tttaatttta atttcatagt taaataagca
     1681 tatattatgc caatatatag aaaaatagct ttattattta ttaaaataaa ataaactcta
     1741 cttagctttc gtaatctttt atttttagct atgtttaaat ataaaaaaat tataaaaacc
     1801 aaaaatccaa atataataat acttctaagt aaatgtat
//
LOCUS       DAMJWS010000195         1805 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMJWS010000195 DAMJWS010000000
VERSION     DAMJWS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.201"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..92)
                     /locus_tag="PC399_003757"
     CDS             complement(<1..92)
                     /locus_tag="PC399_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594535.1"
                     /translation="MKDKWILCPVCGNKTRLKIRADTELKNFPL"
     gene            complement(131..418)
                     /locus_tag="PC399_003758"
     CDS             complement(131..418)
                     /locus_tag="PC399_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594536.1"
                     /translation="MSTLIIGFIVAGVLLIAEYLLCTKFRSPLWGGIIPLLILAGTIY
                     VFASGRIPFERPYIFPFVVVNTLFFGDWGTGRDKYKKIKQTEMEKMKAKDI"
     gene            complement(421..717)
                     /locus_tag="PC399_003759"
     CDS             complement(421..717)
                     /locus_tag="PC399_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HDF2594537.1"
                     /translation="MELDKIPQWILALEQEDATFLKNFALKSGSLKEIAKMYGVSYPT
                     VRLRLDKLIQKIEMCDQQEEEPFQTFIKGLAVDSRIDVETAKIIIEKYKKEKEK"
     gene            complement(806..1711)
                     /locus_tag="PC399_003760"
     CDS             complement(806..1711)
                     /locus_tag="PC399_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2594538.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKSVIALWVVLIASVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQALNTDTIRKDIPVSSAVNGVQIWSVTQADENQFSVIFTVEQLITENGSTKT
                     VRSAYEVTVYVDGSGNMVLTKNPTITSIPVKSGYTPKAKESDGTVDAVTTDEINEFLT
                     TFFKLYPTATAKELSYYVNEGVLKPIGKDYVFSELVNPIYNRIDSQVTVSVAVKYLDQ
                     QTKATQVSQFDLVLEKSGSNWKIIK"
BASE COUNT          485 a          397 c          300 g          623 t
ORIGIN      
        1 aatagtggga aatttttaag ctctgtgtcc gctctaattt tcaacctcgt tttattaccg
       61 caaactggac ataaaatcca tttatctttc aataatgcgt tcctcctatc taagtataca
      121 gttttgtaat ttatatatct ttagctttca tcttttccat ttcagtttgc tttatctttt
      181 tatatttatc cctgccagta ccccaatctc caaaaaataa agtattgact acaacaaacg
      241 ggaaaatata tggtctttca aatggtattc tgccacttgc aaaaacatat attgttcctg
      301 ccaagataag caagggaata atacctcccc aaagaggact tctaaatttt gtgcagagaa
      361 gatactctgc aattaataaa actcctgcaa caataaagcc aataattaat gtactcatgg
      421 ttatttctcc ttttcttttt tatatttttc aataattatc tttgcagttt ccacgtcaat
      481 tcgagaatca accgctaacc ctttaataaa agtttggaaa ggttcttctt cctgctggtc
      541 gcacatttca attttttgaa ttaatttgtc taaacgtaat cgaacagtag ggtaagatac
      601 cccatacatt ttcgctattt cctttaatga accggattta agtgcgaagt ttttaagaaa
      661 agtagcgtcc tcttgctcta aggcaagaat ccattgaggg attttatcaa gctccaaaaa
      721 cacaactcct ttcatattat ctttaacaat attcagtata tcataaataa aattaaagtc
      781 aataagaatt taaacatcaa aataattatt ttataatctt ccagttgctc ccgctttttt
      841 ccagcaccaa atcaaattgt gatacctgcg ttgcctttgt ctgctggtcg aggtacttca
      901 cagccacgga aaccgtcact tgactgtcga tgcggttgta gatgggattt accagttccg
      961 agaatacata atctttcccg ataggcttta acacgccctc atttacataa taggacagct
     1021 ctttagcggt tgcggttggg tagagcttaa agaacgtcgt cagaaattcg ttgatttcgt
     1081 ccgtcgtcac agcgtccacc gttccgtcgc tttcctttgc ctttggcgtg tagccggatt
     1141 tcacgggtat gctggtaatg gtcggattct tcgtgagaac catgtttcca gagccatcca
     1201 catagaccgt gacttcatag gcggagcgta ccgtcttcgt gcttccattc tccgtgatta
     1261 actgttccac agtgaagatc acggagaact ggttttcatc cgcctgcgtg acagaccata
     1321 tctgtacccc attgaccgcc gaagacactg gaatatcctt gcggatggta tcggtattaa
     1381 gtgcctgcaa ttcctttgtc agataaccgc tgattgcctg cgtccttgct tcaatagctt
     1441 ctttgctgtt attccatgtg taataggact tcgcaaagtt cttcacgaaa ttttcaatcc
     1501 cgttggtatc ggtaagtctt aactgtatgg tttctgtttc atgtacggtg tgcatatcaa
     1561 tagcggtaaa attcttatat accccaaaac tcacgcttgc gataagcact acccacaatg
     1621 caatcacaga ttttttatgt gtaccgactt tcacagtacg cactttcttt tctttgacat
     1681 tctctgtctg tttcttattc ttcttaaaca tattttctaa gtcctttctt ttattttact
     1741 cgtcctgcac cgattaagtg ttgttgccag taactattat ttaagtctgc atatcctatc
     1801 ggatt
//
LOCUS       DAMJWS010000196         1804 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMJWS010000196 DAMJWS010000000
VERSION     DAMJWS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.202"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            101..448
                     /locus_tag="PC399_003761"
     CDS             101..448
                     /locus_tag="PC399_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2594539.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(598..1647)
                     /locus_tag="PC399_003762"
     CDS             complement(598..1647)
                     /locus_tag="PC399_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2594540.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
BASE COUNT          608 a          280 c          247 g          669 t
ORIGIN      
        1 ataaaagaat tttattccta ttgtgtaaag tgagaatact attttcttat attggtataa
       61 taatagtaaa tctataatat agaaaagagg tatttctagt atgcatgaaa ataaaaaaaa
      121 tctattagac ggaagcttag ctcaatataa tgatacagca gttccaaaaa taccagtatt
      181 tgctggtaac gatataactt cagaagttta ttattttaaa cctaatcaag ttcttaatgc
      241 tcatagacac cctaatggtg aacaaatatt tgtgttccta aaaggagaag gtaaaatgaa
      301 acttggtgag catgaatgtg atgtaaaaaa cggagatact gtatttgtac caacaggaga
      361 gtggcatgaa attactaatg gaagcaatga agaaatggta gccgttcaaa ttacaaaaat
      421 taatgctgga gcagaataca gaggatagat atataagtac agttagtact ttaatatcta
      481 ttttattata taaactttaa tctcaaatat aaaaagagtc ttttaaaagg ctctttttat
      541 atttttgtta attttcatta taaaaacaag aaaatatcta gctaaaacta atatatttta
      601 tttaatattt tttatagcat tgtttgcaaa ctcagtattt acgatatctt tataaggtgc
      661 ttccttatct agttctttag cttctttcat aacagtcatc aatttcttca aaccttcctc
      721 ttttaataca ggattttgag accatgcatc tattgattta tatctatcta caactgttgt
      781 taaatcttct aaagatatat cttcaaagaa aggttgcata gccttagcta cttctttgct
      841 agatgcttgt tgaatccata attgaccttt ataaacagca tttgtaaatc tttgaattaa
      901 atctttattg tttttcatgt atgatctcgt agtagagtat gctgtaaaag gtatttctcc
      961 agaatctttt cctatagatg taactatata tccactacct tctttttcca ttgctgttgc
     1021 tgtaggttca aatgctgttg taaagtcacc ctctccacct gcaaaagctc cagccattac
     1081 accaaattgt acatcagttc ttacattaac attgcctgct tttagatttt ctccaatagt
     1141 aagacctttt tcttttagaa catattcaaa agtcattagt ggaactccac cttttctacc
     1201 acctaatatt tcggttcctt ttaaatcatt gtaagagaaa ttaggcattt ttttcctagc
     1261 aactagaaaa ctaccatctc tttttgtcaa ttgagcaaag ttcactgcat aatcgttttt
     1321 gttttgatta tatacatata tagatgcttc aggacccatt aaacctacgt ttgcttcacc
     1381 tgaaatcaaa gctgccatag ttttatctgc accttgggta tttataaggt caatctttat
     1441 accttcatct tcaaagaatc ctttcgtaat agcagcatac tgtggagcat aaaaaacaga
     1501 gtgagtaact tctgctatag ttactttatc cagattttta gacgaagaag attcatcttt
     1561 tttgtcatta gagttaaaat cacatccagt tagacatatt gtacttacaa tgatagagct
     1621 tgctattgcg actatttttt taaacataat aaatacctcc cataagacat acgaatagta
     1681 tatagtatta aatatttatt gtagttgtta caaaaatgta tctatactta taatatttct
     1741 acaaaaactt aataataatt ttcagtttta tgtttagttt tttcagtaat gtgtaatatt
     1801 ggat
//
LOCUS       DAMJWS010000197         1773 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMJWS010000197 DAMJWS010000000
VERSION     DAMJWS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.203"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            99..1445
                     /gene="rpoN"
                     /locus_tag="PC399_003763"
     CDS             99..1445
                     /gene="rpoN"
                     /locus_tag="PC399_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF2594541.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT          816 a          159 c          315 g          483 t
ORIGIN      
        1 cccttttgca taaggtatat tttaaatgat agaatataca tatacaggag ggtttttctt
       61 ttataaaata agtttaataa aattgagagg tgagcagaat gaattttaat aatagtctag
      121 aattaactca gtctcagaag ctaataatga ctacacaact taagcaatca ttatcaatat
      181 taaatatgag taagttagag ttagaagaag agataaaaag agaagcagag aataacccat
      241 tagttgaagt cgagaaaagt ggggagataa attgggaaga gtatataaaa gacatggata
      301 ggtcaagacg tttagataga acagagataa gctataatcc tgataatgaa gttaatctag
      361 aaaatttagt taaatattcg tcaaatttat atgaagattt aaaatttcag ataagcctgt
      421 ataaacttac agacaaagaa ttggaagtct gtgagtatat aattgatagt ttagatgagg
      481 atggatattt aagaactgag gaaaaagtaa ttgttgatac cttaaagata gatgaagaac
      541 tttttgaaaa atgtttaatt agtgttcagc agttggaacc tagtggagta ggggcaagaa
      601 gtctttcaga atgtctgatg atacaaatgg ctagtttagg aatatataat gaaattttag
      661 aggaaatcgt tacaaaagac ttaaatttaa ttgcaaataa taaatacaaa gagataagta
      721 aaaagtacaa tatgtcatta caaaaatgtg ttgaactaat aaacataatt aagacattag
      781 acccaaaacc aggaagaact tgttcagtag agaaaagtgt atacattcaa cctgatgtaa
      841 cagtagaaaa aattgatgat gaatttattg tatatttaaa tgaaaaagat tcttatcaaa
      901 taagaatcaa tagttattat aaagacatat taaaaaattc acagtctgat gaaagtgcaa
      961 aagaatttat aaaagaaaga ctaaattcag caacaggact tatgaaaaat atagagagta
     1021 ggaaaactac agtacttaag attgcagaag agatagtaaa agctcaagat gaatttttga
     1081 gaaaagggac aaaatatata aaacctttga aaatgaaaga catagcagaa aaattagaat
     1141 ttcatgaatc tactataagt agaggtgtaa atggtaaata tatgttaaca ccttttggtg
     1201 tatatgaatt tagatacttt ttcagtagtg ccattgagac tgagaataat gaaatggcat
     1261 ctagtacaag cataaaaaaa attataaagg aaacaataaa agacgaaaat aaaaagaaac
     1321 ctttaagtga tgaccatata tctaagttac taaaagaaaa aggaatcaat gttgcaagaa
     1381 gaacagttgc caaatatcgt gaagaattag gaatcccatc ttcaagtaag agaaaagaat
     1441 actaataagt aaaagacaaa tatcagtatg gtaaggggaa aaggctaatt aaaagatgaa
     1501 tgtagattgg gctaaaaatt ttatgaagta aaataataga atatggaaaa gtattaaaat
     1561 gctataagat attaaaaaac tggggaacat taaaacatta agatagcaaa gaacataaaa
     1621 aactaaagaa gattaaaaca ttaaagaaca ttagagcttt gaggaatatt agaactttaa
     1681 agaatattaa aatttataaa atataaaaat actaaaaaga attgaaaaac tggggaacat
     1741 taaaatattg taaaatgcta aaaaactaga aaa
//
LOCUS       DAMJWS010000198         1757 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMJWS010000198 DAMJWS010000000
VERSION     DAMJWS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.204"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            187..1527
                     /locus_tag="PC399_003764"
     CDS             187..1527
                     /locus_tag="PC399_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2594542.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCV
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          701 a          124 c          284 g          648 t
ORIGIN      
        1 taaaaaaagc aataaaaagt ttaattcaat ttagcaaatt tagtgatata attaaaaaag
       61 aaaagaaata tggacttatt tggaataatt ttgacaatct atttactaca cgtgtagagt
      121 cgtatataat taaattatac ataaaatata aaaaaataat ttagtaatac gttaggagaa
      181 agtattatga taaagtcaag cttattttgg aatattttca ttgcgatatc ctttatgata
      241 gaatggtttc tttgtaagaa aatgctagat tatacaagtg agaaaaggat ttctaacaaa
      301 aaaataaact tttgtgtggt aggtattatt gtttttgctg acataatata tttttttgat
      361 gtacctccaa atataagagt aattatatct atgataatta ctattgcatt ttataaaatc
      421 tcatatgatg tggggtttat aaagtctgta ttaatatcct taatttattg gatgttacta
      481 atcgccacag atgctttgag tatatcttta acagtttggc ttaattctat aaatgacatg
      541 agtagtatgg ggacgggaga tatatacaga attgagtcta taatattggg aaagatactt
      601 ttgattgtgt ctttgctttt atatagagcc ttaaagataa aacttaatat agaaataaat
      661 aaaaggacat tattttatat agttattcct atagtcgcta atatagcatc tttttttgta
      721 atttttgaat acatatttaa tccagggtct ggtagcttaa tacataattt ccaattcgtt
      781 atgatttctg ttgtattgct tatatcaagt atcactttag ttttaattgc taaaaaatta
      841 cagcaatata atactttagt tgtagaaaat aatatgatta aaaatacttt gaagtatgaa
      901 aatgagtatt atgaagatat agaaaagaaa tatttaaaaa ctagattact aagtcatgat
      961 atgaaaaatc acatcttgtg tataaatgct atgattaaaa aaggtattga tgttacttct
     1021 tatttaaata atttgcaggt agaaattcag agtaatgact tgttatttaa tactggtaat
     1081 tttatattag atgctatatt aagtgaaaag aaagaggttt gtgataagaa tgatatagaa
     1141 ctaaaaatag gaataaattt tagttgttgt gaagctgttg aattagtgga tatatgtcat
     1201 atattttcaa atattttaga caatgcaatt gaggcatgta ataaaattga tagtggaaat
     1261 agagttattt gtgttaaagg tgatgttatg caaaattttt atttaataag agttgaaaat
     1321 actaagatga ataagattat tactaaaaat ggagatattt tgacagacaa aaaaggttct
     1381 cttcatggac ttggaataaa aagtgtaaaa aactctgtaa ataagtatgg tggtaatgtt
     1441 gtaattgaat atgatgataa aaaatttgta gttaaaattg ctattccact tagaaatgtg
     1501 aagaaagcag ttaatcatgt gatttaaatg tatataaatt tgatgtgtag aaatttaata
     1561 tataagaatt aatgtataaa agtgatgcat agaagtttaa tatataagaa ttaatgtata
     1621 aaagtgatgt atagaagttt aatatataag aattaatgta taaaagtgat gtatagaagt
     1681 ttaatatata agaatttaat atgtataaaa gtgatgcata aaagtttaat atataggaat
     1741 ttaatatgta taaaagt
//
LOCUS       DAMJWS010000199         1698 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMJWS010000199 DAMJWS010000000
VERSION     DAMJWS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.205"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            125..859
                     /locus_tag="PC399_003765"
     CDS             125..859
                     /locus_tag="PC399_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2594543.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNALMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1002..1493)
                     /locus_tag="PC399_003766"
     CDS             complement(1002..1493)
                     /locus_tag="PC399_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2594544.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          636 a          197 c          246 g          619 t
ORIGIN      
        1 atttatatta ttaaaaaatt tgtttataga aacttttatt attttatata aaagtgataa
       61 aaacttatat tttgtattta atagagtgtt gctaagtgaa tatatttaaa atggaggaaa
      121 ctttatgaaa caaaataaat atgatgatga tagatttttt aataaataca gtaaaatgga
      181 acgttctaca aatggattag caggtgctgg agagtggcat gttttaaaaa aaatgttacc
      241 tgatttcaaa ggaaaaagag ttttagattt aggatgtgga tttggatggc attgtcaata
      301 tgcagttgag aatggagcaa tttctgctgt tggagttgat atttcagaaa aaatgttaaa
      361 agaagctaga aacaaaacgc aacttgataa tataaattac atatgtatgc ctattgaaga
      421 tattaatttt ccaaaagata gttttgatgt agttattagt tccttggcat ttcattatat
      481 tcaatcattt gaagatatat gtaagaatat aagtaattgt ctttcaaata aaggtaattt
      541 tgtattttct gttgaacatc ctatatttac tgcatatgga agtcaagagt ggtactgtga
      601 taaagatgga aataatctac actggccagt agatagatat tttgaagaag gagtacgtag
      661 gtcaaatttt ctaggagaag aggttataaa atatcataaa acattaacaa cttatttaaa
      721 tgcgttaatg aaaacaggat ttgaaatatt agaaattatt gaacctcaac cagaagaaaa
      781 actgttagat acaattcctg atatgaaaga tgagctacgt agacctatga tgttacttgt
      841 atctgcaaaa aaaagataag taaatataaa taatcattat tgtagaaaaa aagttgtaga
      901 aaataattac actaatggag atgattatac cagtggtatt aattacacca gtagtaagga
      961 ggcaaaagca ttcgttgctt ttgccatttt ttaagttgca attatacttc tacgtagtat
     1021 atttcgtttc cattttcttc tataagcttt ttcatatctt caaattttaa aaatacagta
     1081 gcagtattat catttggatg tatacctatt ttatccattt ttaataaatc cttatcaaat
     1141 acaactttta caacagcctc tttatcattt aatataccaa aaggagtcac agctccttta
     1201 gtaagtttca agtatttttg aagtctttct tctgatgcaa aactaagagg tttactatca
     1261 atttgttgac gaatatcttt taaatctgct tttttgtctt tacctaatac aactaaaaaa
     1321 tgttcttttc ctttagcatc acgaagaaat aggtttttta caacaaattc catatgtaat
     1381 gtttttaatt cttccatttc ttcaattgta taaactggtg gatgatttac agcctcataa
     1441 ggtatgctta actcatctaa ttttttatat acttcctgtt gttgattgtt catgatttcc
     1501 tcccattaga aatttatttt atatataaat aatattatca cagaaaactt taaaagaaaa
     1561 cgttttatat aatatagaca atagattata tttttataaa gaagcaagaa tactcaataa
     1621 aacttaaaga aattaaacga tttctaaaaa taccttgaaa atagaacctt atttgccatt
     1681 tataaattat ataaaaag
//
LOCUS       DAMJWS010000200         1681 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMJWS010000200 DAMJWS010000000
VERSION     DAMJWS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.206"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            602..1309
                     /locus_tag="PC399_003767"
     CDS             602..1309
                     /locus_tag="PC399_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2594545.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          710 a          145 c          286 g          540 t
ORIGIN      
        1 ttaaaaaaat agaattatta tatttatgat aataaatggt ataataaaag gtaaagtgtc
       61 aaatatgcac taaattaaat aagttaatta gtatataggt taaagtggaa gtaattaaaa
      121 cttattaagt attagaaaat gacttcttat tagagtaagt atctaaaatg atataaaaat
      181 tatgattact cattaaagtg actattatag agtggataag attactcatt aaagcgatta
      241 ctgtggagta gataaaatta ctcactaaaa taactatcat agagtggata agattactca
      301 ttaaagcgat tactgtagag tagataaaat tactcactaa aataattatc atagagtgga
      361 taagattact cattaaagcg attgctgtgg agtagataaa attactcact aaaataacta
      421 tcacagtaga taagattatt cattaaagtt actatcacag agtggataaa attacacatt
      481 aaaattgtct gttaaagaat gaataaaaaa gagattgctc aatataagaa gcatataaaa
      541 aataaaaaat caaacgtata aaatttaata aataataact gatagggaaa ggaaagatag
      601 aatgatattt acaaaaagta ataaatacga taaggacttt ctcatgaaaa atatgatggg
      661 acctaattgt ataaaaatat tggaagaatt aacaagtaag ataaaactag aaaagggaat
      721 gcgtatttta gacttaggtt gtggtaaagg tatatcttcg atatttttag caaaggaatt
      781 tgatgctact gtatttgcaa cagatttatg gatagaacca actgaaaact ataaaagatt
      841 taaagagttt aaattggatg ataaaatatt tccaatacag gcagaggcac atgaactacc
      901 ttatgcagaa ggtttctttg atgcggtgat aagtatcgat tcttatcact attttggaaa
      961 caaagaagga tttttagaca atcatatatc acctcttgta aaagaggggg gaatacttgc
     1021 tatggcaatg cctggattaa aagaagattt cgtggattgt ataccagatg aactcattcc
     1081 attttggcaa gataatatga acttccattc aattacatgg tggaataaac tttggtctga
     1141 atcggagtct gtgattgtag agaaatgtga agctttaaat tgtcatgatg aagcttggaa
     1201 ggattggata aattgtgata attcatatgc tataaatgac aaaaaaatga tggaagtaga
     1261 gaatggaaag tattttaata caatttcttt gatagcgaga actaaataat tgtgatgaat
     1321 ttataaaagt ataagataag tagaaatata agttgaatga gagtataaga agcatagaaa
     1381 tataagttga attaaagtag aaggtaaata aaaatgtaag ttaaataaag agtttataca
     1441 aaaatgattt tttagattat ttatacccaa tcgagttgct taggtcagtt gggtattttt
     1501 attttttaaa gttaaattgc aaatactatt gtgtgatata tattttaata ctaaatgttt
     1561 gttttataag aaataaacta atagaaaaaa tagttttaac aatgaaagca aaaaaggtat
     1621 tgatgaagat taaaaatttt atagtaccat tttatatatt ttattatact ttaaatcaaa
     1681 t
//
LOCUS       DAMJWS010000201         1595 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMJWS010000201 DAMJWS010000000
VERSION     DAMJWS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.207"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            46..1506
                     /locus_tag="PC399_003768"
     CDS             46..1506
                     /locus_tag="PC399_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF2594546.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSA
                     RLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVN
                     TVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTL
                     VNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLV
                     NDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGM
                     YKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYI
                     KIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLG
                     NAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT          653 a          166 c          270 g          506 t
ORIGIN      
        1 tgtatttata tattaaaaat ttattatgag ggggacaatt ttattatgaa attctataaa
       61 agaatattaa cattgacttt aagtatttct tttgtatttg caaatattca attagtagat
      121 gcaatatcat ctgtagaaaa aatacaagga aaaaataagt atgaaatagc aggtaaaata
      181 gcagataaaa atgcatataa aactgcatat aaaactgcat ataaaactgc tatattaatt
      241 aatactagta actctattgc agatggatta agtgcaagtg gactagcagg agctttaaat
      301 gcccctatac tattaactga aaaaaatact ataccaactg agacatctgc aagattaaaa
      361 aatgtatcaa aagtttatat aattggagga acatattcta ttagtacatc tgtcgaaaat
      421 tcattaaaat ctaagaaaat gaaagttgta agaattaagg gaaatgacag aataaaaaca
      481 agttataatg ttgcaaaaga aataaattca ataaaaaaag tcaatactgt tatgctaacc
      541 aatgcatata aaggagaagc agatgctata agtattgcat ctgtagcagc aagagataaa
      601 gcaccaataa ttcttactaa tggacaaagt ataccttttt caactagtgg tttaaagtct
      661 tatgtaatag gaggaacagc ttcaatgagt accactttag taaacaatac taaatctact
      721 agactagggg ggagtactcg ttttgaaact aataaagcaa taataaataa gttttataag
      781 gatgctagag agttttatat agctggagct tatgaattaa caaatgcact tgtaggatct
      841 tctttatcaa aacatggtcc aatggtttta gttaacgatg gtagcaataa aagcatttta
      901 aaaaatgcta agaagataac ttctatagga tatatagatt caaatatagt acaacagtgt
      961 ttaaatataa caaatggaat tggagatatt aatactgggg ttgtaaaaaa tgttaaacct
     1021 actacaaaga ctataaaaga tggaatgtat aaggttggaa aagatatctc agcaggtgaa
     1081 tacttaataa cttcaaactc aggaagttat gcttcttatt atgaagttac aagtgatagt
     1141 acaggtaatg ctgatagtat actttcaaat gatattttta gtgggactag atatatcacc
     1201 ttaaagaatg gtcaatatat taaaattgag gattcaacaa tgacattagc aaagtatgct
     1261 aaagcccaga aggccaagaa tggtaagttt ggaaatggta tgtataaaat tggattagaa
     1321 attcctgctg gagaatatat tataatgtca aattcttctg atgcatatta tgaagttaga
     1381 aatgatagct taggtaatgc agagggaata gttactaatg atactttttc aggaagaaga
     1441 tatataacgg ttgaggaaga tcaatattta atattaaatg attgttattt aattgaaaat
     1501 gaatagatgt aattgtattt aatagtaaat catatatttt atttaaggaa aggtgtttta
     1561 agttttaatt taaaacatct ttttattatt ataat
//
LOCUS       DAMJWS010000202         1592 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMJWS010000202 DAMJWS010000000
VERSION     DAMJWS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.208"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            260..1531
                     /gene="serS"
                     /locus_tag="PC399_003769"
     CDS             260..1531
                     /gene="serS"
                     /locus_tag="PC399_003769"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2594547.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKNFNLDEVVKLDDERRKTLQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          665 a          165 c          290 g          472 t
ORIGIN      
        1 agtcaaaata gataattgat tagcaaaata gatataatta taatgaagtt aaatctagca
       61 atattctcct aaaattaaat ttcatttatt taattaaata taatttgaca atgggcaaaa
      121 aagtgagtaa aatataaata aaataaaaat aacaggttcg gataaagaaa aaggtttctt
      181 aacctcaagc ataaagtata ttatctaagt tatttgagat taagagataa atccaatctt
      241 aggtactgag gaggaaaaaa tgcttgatat aaaaagaata agagaaaatt tagattgtat
      301 aaaaaaagca atggaaagaa ggggcgaaaa aaactttaac cttgatgaag tagttaagtt
      361 agatgatgaa agaagaaaga ctcttcaaga agttgaagtt atgaaaaatg aattaaatac
      421 agcatctaaa aatataccaa atcttataaa agaaggtaaa gatgtagaaa atgaaaagat
      481 taaattaaaa gagttatctg ataaaattaa ggtaatagac caaaatctta aagaagtaga
      541 agataagatg gaatacctat taatgagaat accaaatgta ccacatccag aagtaccaca
      601 aggtgaaact gatgaagata atgttgaagt aagaacttgg ggaaagacta ctacctttga
      661 ttttgaatct aaggctcatt gggaaattgg aacagaatta ggtatacttg attttgaaac
      721 agctagtaaa ataactggtt caagatttac tctttacaaa ggattaggtg ctagattaga
      781 gagagcatta ttaaattttt acttagacac gaatacaaaa gtaaatggat atacagaagt
      841 tattccacca tttatggcaa atagaaatag ttttttaggt acaggacagt tacctaagtt
      901 tgaagaagat atgtttaaaa tagaaggtat ggactatttt atgattccaa catcagaagt
      961 tcctttaact aatatacatg ctaatgaaat acttaaattt gaacaattac cgataaatta
     1021 tacagcttat actccatgtt ttaggtcaga agctggttct gctggaagag atacaagagg
     1081 tttagtaaga cagcatcagt ttaacaaagt agaaatggtt aaaatagttg caccagaaga
     1141 atcttacaat gagttagaaa agcttactaa caatgcggaa actatgctac aattattgaa
     1201 tctgccttat agagtagtaa agatatgtac aggtgattta ggatttacgg cttcttttaa
     1261 atatgatgta gaagtttgga tgccaagtta caataggtat gttgagattt catcttgttc
     1321 aaactgcgaa gatttccaag ctagaagagc aggaataaga tttaaaagag ataaagattc
     1381 taaagctgaa tatgtacaca cattaaatgg ctctgggtta gctattggaa gaagtgttgc
     1441 agctatatta gaaaattatc aacaagaaga tggttctgtg gttgttcctg aagttttaag
     1501 accatatatg ggagtttctg ttattaaata agagttaaat tatgtatttt taagtaatac
     1561 aataatatag aggtaataaa aaataattaa ta
//
LOCUS       DAMJWS010000203         1535 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMJWS010000203 DAMJWS010000000
VERSION     DAMJWS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.209"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(614..1369)
                     /locus_tag="PC399_003770"
     CDS             complement(614..1369)
                     /locus_tag="PC399_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HDF2594548.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT          534 a          244 c          121 g          636 t
ORIGIN      
        1 tatggttaat ttatacaact ataattaata aaagagtata ttttaaatct aaaagctata
       61 ggttgaaaca tactcttttt attttgttta tttatagctt tagttattaa tcatatataa
      121 atctacaaaa cgtattccta gtaacttata ttttttgata tctacaatca taagactata
      181 ttattggatt catacctcaa aaatattgcc ttctgaacat tattatattc aaagctatgt
      241 taatttacaa tgatatatcc taacatgtat tgattaaaaa aaattaaaac ttgcttttca
      301 tctcctatat ctaaagaagt ggtatttaga ataaactatt gtaatattaa agttgaaatt
      361 ttctagttta tttactctat agcaccatat tttaatacta ttaagtaaaa atgtagttaa
      421 aatatatact aatatccctg aaataagtat ttctttttta tttagcacac ttattcgtaa
      481 ggtattagtt attatatttc caaattatag aataaatatt atacttattg ctaattataa
      541 tattaatact gttaagatat ttttcttcat agttataccc attatttaat acaatttatt
      601 ataaattcaa tttttaaact tttcttatct ccttatcaga ataaaaatcc cctttacttc
      661 cttttatttt tactattttt aaacttgcca tcttctctaa tctccaaagt ataaaccaat
      721 cttttacacg catttcatct tcaaacatac agtttgcaac cacttcaatt aaaggttgcc
      781 atttgtcact gatatttctt aatatatatt tatcaaaata agtttcttca acacttttaa
      841 tttcgttacc tactaatgct ctaaaaatag aattttcttt aactaacaca tcccatttat
      901 ttttaaattc tagtttttca ttaaagccaa tttctagtgt cttatctaaa aagaatggaa
      961 aatcttctgg agacatttca ctggtaaatg aatatctaat tagagcatct tctgtctttt
     1021 tttcatctac taatgcaatc tttatatttt tatcttgaaa cttactcact acataatata
     1081 ctccacacaa atcttttggg ttatttgcac accatattat tatatttcta cattttgata
     1141 atttcctatc ttctaacaaa tctggcttga ataatagttt cggattattt tcaaaaacat
     1201 ttctaatctt aaaaatattt tctcttaatt cgtagttatt tacatcaaaa atattaccta
     1261 ttgataaatc atcttcaaat aatacaattt tattatgtgg tattaacttt aacttcattg
     1321 cttcttttaa taacaaataa caaatatcct caaagcatat atgtgccata aaattcacct
     1381 cttttaaaat taacaattta ttttattata atatataaac tgtaattctg aacctcccat
     1441 gactaaagtc acagggttcc tgcttcacag aattttgcat actaaaatat gtcttactaa
     1501 ttctccacag gctatccccg tagttcctac ggttc
//
LOCUS       DAMJWS010000204         1471 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMJWS010000204 DAMJWS010000000
VERSION     DAMJWS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.210"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            386..>1471
                     /locus_tag="PC399_003771"
     CDS             386..>1471
                     /locus_tag="PC399_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HDF2594549.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGVTGEIGPTGVTGPTGVTGSIGPTGATGPTGATGVTGEIGPTGATGPTGNT
                     GVTGATGPTGNTGVTGVTGATGPTGATG"
BASE COUNT          599 a          198 c          292 g          382 t
ORIGIN      
        1 attccattta acaaaaataa agttttttat aatttttact tataaaaaaa tattgttatg
       61 gtaatatatt ctatttacaa ttttataact ttggtttaca ataatataga caataaatcc
      121 taaaaaacat aataagaaaa aagtgcttaa tattttagaa agactggttt agtttacacc
      181 agctaagcca gtcaaggaat aatttaaagt gtaatatata ataatgaaaa agaacatgct
      241 attagaaatt tctaatgaca tgttcttttt ttgtagaaat cagcattatt atataaaaat
      301 ttaatactat aaattttaat tgtattgtaa ctaagcatgt attttaaaca tagtatgata
      361 tagacccaaa atggagggat tataaatgag aaatattata ctttatttaa atgatgatac
      421 ttttatatct aaaaaatatc cagataaaaa ctttagtaat ttagattatt gcttaatagg
      481 aagtaaatgt tcaaatagtt ttgtaaaaga aaagttgatt acttttttta aagtgagaat
      541 accagatata ttaaaagaca aaagtatatt aaaagcagag ttatttattc atattgattc
      601 aaataagaat catattttta aagaaaaagt agatattgaa attaaaagaa taagtgaata
      661 ttataattta cgaactataa catggaatga tagagtgtct atggaaaata tcaggggata
      721 tttaccaatt gggataagtg atacatccaa ctatatttgt ttaaatatta cgggaactat
      781 aaaagcatgg gcaatgaata aatatcctaa ttatgggtta gctttatctt taaattaccc
      841 ttatcagatt cttgaattta catctagtag aggttgtaac aaaccgtata tacttgtaac
      901 atttgaagat agaattatag ataattgtta tcctaaatgt gagtgtcttc caattagaat
      961 tacaggtcca atgggaccaa gaggagcgac aggaagtaca ggcccaacag gagtaacagg
     1021 cccaaccgga agtacaggag cgacaggaag cataggacca acaggcccaa ccggaaatac
     1081 aggagcaaca ggaagtatag ggccaacggg agtaacaggc ccaaccggaa gtacaggagc
     1141 gacaggaagt ataggaccaa caggagtaac aggtccgaca ggaaatacgg gagtgacagg
     1201 aagtataggc ccaacgggag caacaggccc aacaggagtg acaggagaaa taggcccaac
     1261 gggagtaaca ggcccaacag gagtgacagg aagtatagga ccaacaggag caacaggtcc
     1321 aacaggagcg acaggagtga caggagaaat aggaccaacg ggagcaacag gtccaacagg
     1381 aaatacagga gtaacaggag caacaggtcc aacaggaaat acaggagtaa caggagtaac
     1441 aggagcaaca ggtccaacag gagcgacagg a
//
LOCUS       DAMJWS010000205         1354 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMJWS010000205 DAMJWS010000000
VERSION     DAMJWS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.211"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            44..118
                     /locus_tag="PC399_003772"
     tRNA            44..118
                     /locus_tag="PC399_003772"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Thr,seq:tgt)
     gene            133..217
                     /locus_tag="PC399_003773"
     tRNA            133..217
                     /locus_tag="PC399_003773"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:167..169,aa:Tyr,seq:gta)
     gene            227..310
                     /locus_tag="PC399_003774"
     tRNA            227..310
                     /locus_tag="PC399_003774"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:261..263,aa:Leu,seq:tag)
     gene            340..416
                     /locus_tag="PC399_003775"
     tRNA            340..416
                     /locus_tag="PC399_003775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:374..376,aa:Arg,seq:tct)
     gene            424..499
                     /locus_tag="PC399_003776"
     tRNA            424..499
                     /locus_tag="PC399_003776"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:457..459,aa:Gln,seq:ttg)
     gene            588..676
                     /locus_tag="PC399_003777"
     tRNA            588..676
                     /locus_tag="PC399_003777"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Ser,seq:tga)
     gene            680..755
                     /locus_tag="PC399_003778"
     tRNA            680..755
                     /locus_tag="PC399_003778"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:713..715,aa:Phe,seq:gaa)
     gene            762..835
                     /locus_tag="PC399_003779"
     tRNA            762..835
                     /locus_tag="PC399_003779"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:796..798,aa:Met,seq:cat)
     gene            850..926
                     /locus_tag="PC399_003780"
     tRNA            850..926
                     /locus_tag="PC399_003780"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:884..886,aa:Ile,seq:cat)
     gene            950..1026
                     /locus_tag="PC399_003781"
     tRNA            950..1026
                     /locus_tag="PC399_003781"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:984..986,aa:Pro,seq:tgg)
     gene            1034..1110
                     /locus_tag="PC399_003782"
     tRNA            1034..1110
                     /locus_tag="PC399_003782"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1068..1070,aa:His,seq:gtg)
     gene            1119..1194
                     /locus_tag="PC399_003783"
     tRNA            1119..1194
                     /locus_tag="PC399_003783"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1152..1154,aa:Lys,seq:ttt)
     gene            1202..1275
                     /locus_tag="PC399_003784"
     tRNA            1202..1275
                     /locus_tag="PC399_003784"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1234..1236,aa:Cys,seq:gca)
     gene            1282..1353
                     /locus_tag="PC399_003785"
     tRNA            1282..1353
                     /locus_tag="PC399_003785"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1314..1316,aa:Asn,seq:gtt)
BASE COUNT          324 a          311 c          363 g          356 t
ORIGIN      
        1 gaggtcgagg gttcgagccc cttccaggtc gccaaaataa aatgctggtg tggctcaacg
       61 gtagagcagc tgacttgtaa tcagcaggtt gtaggttcga ttcctatcac cagctccata
      121 taaaattaat acggaggatt tcccgagcgg ccaaaggggg cagactgtaa atctgttgtc
      181 atcgacttcg gtggttcgaa tccaccatcc tccaccaaaa aaatgcgcgg atgtggcgga
      241 attggcagac gcactagact taggatctag cgccattggc gtgggggttc gactcccttc
      301 atccgcacca tttttgtttg atatacatat atatattgtg ggtgcatagc tcagctggat
      361 agagcaacgc ccttctaagg cgtgtgtccg gggttcgaat ccctgtgcgc tcaccacata
      421 tattggggat tagccaagtc ggtaaggcac atgactttga ctcatgtatg cgtaggttcg
      481 agtcctgcat ccccagccaa atgatgtatt actaagctat atttgaatta tttttatttt
      541 aaatatagtt aggttcaatt ttagaataag tttaagtatc cgaatgtgga gaggtgtccg
      601 agtggtttaa ggagctggtc ttgaaaacca gtgacttcga aaggggccgt gggttcgaat
      661 cccaccctct ccgccaaatg cccagatagc tcagtcggta gagcagggga ctgaaaatcc
      721 ccgtgtcggt ggttcgattc cgcctctggg caccaaaata tggcggtata gcttagttgg
      781 ctagagcgtt cggttcatac ccgaaaggtc ataggttcga ctcctattac cgctactaaa
      841 atttaatgtg ggccattagc tcagttggtt agagcgcccg gctcataacc ggtaggtctg
      901 gggttcgagt ccctgatggc ccaccacatt aaattaatta atataaattc gaggtgtagc
      961 gcagtttggt agcgcacatg gtttgggacc atggggccgg gggttcgagt cccttcacct
     1021 cgaccaatat aaggtgggta tagctcagtt ggttagagcg ccagattgtg gctctggagg
     1081 tcgtgagttc gactctcatt atccacccca attcatatgg tctattagct cagtcggtag
     1141 agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc accaaaaagg
     1201 tggcgacata gccaagtggt aaggcagtgg actgcaactc cttgatcccc agttcgaatc
     1261 tgggtgtcgc ctccaaaata ttccaaggta gctcaacggt ggagcaaccg gctgttaacc
     1321 ggtaggctgt gggttcgagc cccacccttg gagc
//
LOCUS       DAMJWS010000206         1349 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMJWS010000206 DAMJWS010000000
VERSION     DAMJWS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.212"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>1349
                     /locus_tag="PC399_003786"
     rRNA            <1..>1349
                     /locus_tag="PC399_003786"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          357 a          292 c          408 g          292 t
ORIGIN      
        1 caaggtttcc aggggaaggt tcgtccgccc tgggttagtc gggacctaag ctgaggccga
       61 aaggcgtagg tgatggacaa caggttgata ttcctgtact accgataacc gtttgagaga
      121 agggatgaca cagtaggata agctaagcac actgttggtt atgtgtgccc aagcattgag
      181 gcagtcaaag taggcaaatc cgctttgata atgctgggat gtgatgggga gcgaaattta
      241 gtagcgaagt agctgatttc acactgtcaa gaaaagtctc tatcgaggtt aaaggtaccc
      301 gtaccgcaaa ccgacacagg tgggtgagga gagtatccta aggccagcga gagaactgtt
      361 gttaaggaac tcggcaaaat gaccccgtaa cttagggata aggggtgcca ccatcaggtg
      421 gccgcagaga ataggcccaa gcgactgttt accaaaaaca taggtttctg ctaagtcgca
      481 agacgatgta taggagctga cgcctgcccg gtgctggaag gttaagggga tctgttagag
      541 caatcgaagc agtgaactta agccccagta aacggcggcc gtaactataa cggtcctaag
      601 gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaac gatttgggca
      661 ctgtctcaac aacagactcg gtgaaattgt aattccggtg aagatgccgg atacctgcga
      721 caggacggaa agaccccatg gagctttact gtagcttgac attgggtctt ggtactacat
      781 gtacaggata ggtgggaggc tttgaaacca ggacgccagt tttggcggag ccatccttgg
      841 gataccaccc ttgtagtact gggactctaa ccataggcca tgaatctggt cttgggacac
      901 tgtcaggtgg gcagtttgac tggggcggtc gcctcccaaa aggtaacgga ggcgctcaaa
      961 ggttctctca gtacggtcgg aaatcgtacg tagagtgtaa aggcaaaaga gagcttgatt
     1021 gcaagacata caggtcgagc aaggatgaaa atcggactta gtgatccggt ggttctgcgt
     1081 ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt atctccccca
     1141 agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcatcaca tcctggggct
     1201 gtagtaggtc ccaagggttg ggctgttcgc ccattaaagt ggtacgcgag ctgggttcag
     1261 aacgtcgtga gacagttcgg tccctatccg tcgcaggcgt aggaaatttg agaagacctg
     1321 tccttagtac gagaggaccg ggatggacg
//
LOCUS       DAMJWS010000207         1322 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMJWS010000207 DAMJWS010000000
VERSION     DAMJWS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.213"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            185..1282
                     /gene="ychF"
                     /locus_tag="PC399_003787"
     CDS             185..1282
                     /gene="ychF"
                     /locus_tag="PC399_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF2594550.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          513 a          144 c          241 g          424 t
ORIGIN      
        1 ttatttaaat taaatacaaa tattatttaa atcaatatgc tttaaagtct agtttacaag
       61 cttgatatat atgatgtata atcaatatgt atgtctataa ttaaaacaat tttgcaatcc
      121 tatagattga ttgtttaaag ttatataaag aatataaaaa agaataagag agaggacgat
      181 taatatgaaa ttaggtatag ttggtttacc aaatgtaggt aaaagcacac tatttaacgc
      241 aataactcaa gcaggtgcag aatctgcaaa ttaccctttt tgtacaatag acccaaatgt
      301 gggagttgta tctgttccag atgaaagatt aaacaaacta caagaattat ataattctga
      361 aaaaatagtt cctactgcta tagaattttg tgatatagca ggtttagtta gaggagcttc
      421 taaaggagaa ggtttaggaa ataaattctt atcacatata agagaagttg atgcaatagt
      481 tcatgttgtt agatgttttg aggatgaaaa tgttgtacat gtagatggtt ctgtagaccc
      541 acttcgtgat atagaaacta taaacttaga attaattttc tcagatatag agattcttga
      601 aagaagaatt aataaaactc aaaaagctgc taaggctgat aaaactttag gttctgaact
      661 tgatttatta aaatcaataa tgtctacttt agaagaaagt aagtgtgtaa gaactatgga
      721 atttacagaa gatgaacaaa cattcgtaaa ttcattggat ttacttacat ctaagcctgt
      781 aatatatgct tcaaatgtat cagaagatga tttagctgat aatggagaaa ataataaata
      841 tgttcaacaa gttaaagcat ttgcagaaac agaagatgcc gaagttgttg tagtatgtgc
      901 acaaatagaa gcagaaattt ctgaacttga ttctgctgaa gaaaagaaag agtttttaga
      961 aacattaggt cttgaacaat cagggcttga taagcttata aaatcttctt atgctttact
     1021 tggtttaatt tcattcttaa ctgctggtcc aaaagaagtt agagcatgga ctataaaagt
     1081 tggaagtaaa gctcctcagg caggaggaaa aatacattct gatatagaaa gaggatttat
     1141 cagagcagaa acaatagcct ttaatgattt ggttgagcat ggtactatgg ctgctgctaa
     1201 agaaaaaggg ttggttaaat tagaaggtaa agaatacata gttaaagatg gagatgtaat
     1261 attatttaga tttaatgttt aaaatgatta atcgtataca ctttgactat tatatataat
     1321 aa
//
LOCUS       DAMJWS010000208         1311 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMJWS010000208 DAMJWS010000000
VERSION     DAMJWS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.214"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            49..780
                     /locus_tag="PC399_003788"
     CDS             49..780
                     /locus_tag="PC399_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594551.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(937..1143)
                     /locus_tag="PC399_003789"
     CDS             complement(937..1143)
                     /locus_tag="PC399_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594552.1"
                     /translation="MSKYSSLWEYFKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          548 a          136 c          153 g          474 t
ORIGIN      
        1 aatttatttt tcacaagtac aatacttatt taaaaaagga ggtttaatat gttaagtaaa
       61 aaaaatgtaa gtttaattat tttagtaata attttaagtt tattaactgc tatatttatt
      121 ttcaataata aaaatactaa caataaattg aaagaagtta ctaagaataa tgaagaaata
      181 gtacctaaaa acttagaagg tttaagtata gaacaattaa tagagattag agataaagcc
      241 attgataaaa atttagaagg tcttagggaa tttggagaag attcagtaaa aaaacgtaaa
      301 gattcaattt taaactttga atttggatta gtagcttttg ttaacaagaa ttatagcaac
      361 ttaacagaaa aagagaaatt aataagacaa attaatattg ttaactatga aaactctata
      421 aatgatataa tggatccttc aggttcattc tttgaattag caaatcagaa ttaccaagtg
      481 aatgagtttc tattaaaaaa cggaaaagat tataaagaat ggtcatatga aggtctagat
      541 tttctaattg atcatctaga agaactgtgc aaatttgttg attttgatgt aaaagatgta
      601 atagatatta ttgatcctac aataaaaaca gacctttcag aggaacaaaa gaaatctttg
      661 aatgataaat taaagaaaat atcttcaagt gatactttaa gtgaagaagt taaaaaggag
      721 ataaaaaact gggaaaacaa tttaaataat ttaaatatga ataaaaataa ttctaattaa
      781 ttttctcata agaaaaatgc ttatatcgtg ataatgtgaa atgtacataa ttataaacaa
      841 aaaaggggct gtgacactaa aaaatagtgc tacgcccttt tttatttgta ataatcatca
      901 atacaaatta ttttattata tgtaattatt tatgtcttaa ccaattttat cgaaaataac
      961 agtcttctct tttaaagaga tttttcctac ttgatatcca tattcattta attccttttt
     1021 atatttcaaa aacgaatggt ctatctctat cccagcaata tcttttattt cttcaaaagt
     1081 taatttaaac gattgactat tgtttttctt aaaatattcc cataaagaac tatatttact
     1141 catcttatat cactcctttt aaaatcatat cttatctatt aatttatatt ctatcattat
     1201 taatgtataa agtcatgcta cttattattt atatataaaa atatccaaat aataagtagc
     1261 aattttttat agtcataata tttttttcag attaaataaa aatcaattat a
//
LOCUS       DAMJWS010000209         1260 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMJWS010000209 DAMJWS010000000
VERSION     DAMJWS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.215"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="PC399_003790"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="PC399_003790"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            179..253
                     /locus_tag="PC399_003791"
     tRNA            179..253
                     /locus_tag="PC399_003791"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Asn,seq:gtt)
     gene            259..344
                     /locus_tag="PC399_003792"
     tRNA            259..344
                     /locus_tag="PC399_003792"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Leu,seq:taa)
     gene            360..435
                     /locus_tag="PC399_003793"
     tRNA            360..435
                     /locus_tag="PC399_003793"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Met,seq:cat)
     gene            443..517
                     /locus_tag="PC399_003794"
     tRNA            443..517
                     /locus_tag="PC399_003794"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Glu,seq:ttc)
     gene            532..605
                     /locus_tag="PC399_003795"
     tRNA            532..605
                     /locus_tag="PC399_003795"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Gly,seq:tcc)
     gene            611..686
                     /locus_tag="PC399_003796"
     tRNA            611..686
                     /locus_tag="PC399_003796"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:644..646,aa:Val,seq:tac)
     gene            692..768
                     /locus_tag="PC399_003797"
     tRNA            692..768
                     /locus_tag="PC399_003797"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Asp,seq:gtc)
     gene            779..853
                     /locus_tag="PC399_003798"
     tRNA            779..853
                     /locus_tag="PC399_003798"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Gly,seq:gcc)
     gene            863..936
                     /locus_tag="PC399_003799"
     tRNA            863..936
                     /locus_tag="PC399_003799"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Arg,seq:tct)
BASE COUNT          365 a          260 c          293 g          342 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc atttcgaaca cagaagttaa gctccctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg ttaaatgttc
      181 caaggtagct caacggtgga gcaaccggct gttaaccggt aggctgtggg ttcgagcccc
      241 acccttggag ccaaaaaagc cgaagtggcg gaactggcag acgcacagga cttaaaatcc
      301 tgcgggactt acctctcgta ccggttcgat tccggtcttc ggcacttata aaacaatatc
      361 gcggggtgga gcagttggca gctcgtcggg ctcataaccc gaaggtcgta ggttcaagtc
      421 ctgcctccgc aaccattaaa acggctcgtt ggtcaagagg ttaagacacc gccctttcac
      481 ggcggtaaca ggggttcaat tcccctacga gtcaccaatt caattaaata tgcgggtgta
      541 gctcaatggt agagttctgg ccttccaagc cagctgtgag ggttcgatcc ccttcacccg
      601 ctccaaaaat gggaccatag ctcagctggg agagcacctg ccttacaagc agggggtcac
      661 aggttcgagc cctgttggtc ccaccattcg cggcctggta gttcagttgg ttagaatgcc
      721 agcctgtcac gctggaggtc gagggttcga gccccttcca ggtcgccaaa ttaaataagc
      781 gggaatagtt cagtggtaga gcgcaacctt gccaaggttg aagtcgcgag ttcgaatctc
      841 gtttcccgct ccataataac ttgggtgcat agctcagctg gatagagcaa cgcccttcta
      901 aggcgtgtgt ccggggttcg aatccctgtg cgctcattaa gaaaagttta ctataaatta
      961 tagtaaactt ttatattgtt taaaaagaca caggaaacat gtatattacc tgtgtctttt
     1021 tatttataaa ctttaaacta tgtaaaaatt acagtatatt ctaatagagt tacagtatat
     1081 tcattaatat tgtaaacata cggagggtaa tgtataatag tctacaagat gtttatgaag
     1141 gcatcagtta aatataataa atataactaa ttagtaagag ttatatacat tatattgtat
     1201 actttatggt atttatatat tgaaataact tatgaaatta tttataaaga gattcatgag
//
LOCUS       DAMJWS010000210         1196 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMJWS010000210 DAMJWS010000000
VERSION     DAMJWS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.216"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            200..>1196
                     /locus_tag="PC399_003800"
     CDS             200..>1196
                     /locus_tag="PC399_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2594553.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNF
                     KIGILLFFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSF
                     LQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIANLIYGIGLSIFLG
                     YVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYTPFLFTK"
BASE COUNT          432 a          105 c          149 g          510 t
ORIGIN      
        1 aatagtaaac tgattgcata aaaatgattt ttatataaat tttaaacaga agaataaatt
       61 tctaacgaaa ttggatattt cttctgtaaa tacatattca aaaaaagaat tataagagtt
      121 tagtcagcag tcgtgtaagt aataaggact ttataaatat atagaaatat aatgatatga
      181 gtataagtag aaaaatttta tgatttttac aattattttt tataaaaaag ggaggttatg
      241 tttgttgaat ttacaatcaa aatgggtaaa ctttttaaag ccaacatatg tcagtgatga
      301 aataaaaaga aaaaaactac aagttataat atttttttct ataacttatg gtttatctta
      361 ttcactaggt ttattagcaa tatgttttaa tcatttaata gattcagaaa ttttagctca
      421 atttatgatg atattgccat tatcttcagt ttctattgca aaattttaca cagagggaag
      481 aaataatgat aaatataatt tctattcatc agtaatatta ttttttatta tttacttttt
      541 tatatttatt attgaatcat taaagttaat aagtattaat caatttcaat taatgtatct
      601 tatgttaatt ttaataagta gtttatgtgt tattgtttat tcttataaaa taacaagttt
      661 atatattttt aaaaatttta agataggtat tttgctattt ttttactttt tgttttctca
      721 aattattttt ggattaataa taagtgataa tcaatttgat tataagaatt tcttgaatta
      781 tattcttctt cctataatat cattagtata catttatcct tttttatgtg aagagtatgg
      841 ttggagatct tttttgcaaa gtattttatt tgatagattt ggcaaaaaaa tagggattgt
      901 aatggtaggc acttgttgga gtttatggca cttaccttta caatttacac tatatagtcc
      961 tcaaactcca ataattggag ctatagccaa tttgatatat ggtattggac tttctatatt
     1021 tttaggttat gtttatatga aaactaagaa catatggttt tgctctataa tgcatgttct
     1081 aattaatagc ttaggattaa tttttaatga ttctgaaata attattaatt accatagtat
     1141 tatacaaaga ttgatattta tactattatt ttatactcct tttctattta caaaag
//
LOCUS       DAMJWS010000211         1125 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMJWS010000211 DAMJWS010000000
VERSION     DAMJWS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.217"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     misc_binding    87..350
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            501..1052
                     /locus_tag="PC399_003801"
     CDS             501..1052
                     /locus_tag="PC399_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF2594554.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          448 a          120 c          188 g          369 t
ORIGIN      
        1 tacaaaatat attttttata aaaaggtatt gacatttttt agaaaaaaga atattattat
       61 tttaaaacaa aattcaatac aataaattaa atcctatgac aaggaagagt agttaaattt
      121 aggttcagta tagagagctg aggatggtgg aatcttagca agaaaaaatt tgatgaatgg
      181 cccttagagg aataaattga aatcatagtt ttgagagtag actttatcgt cagtcgcacg
      241 ttatagcgaa taaggtatgt aagtacctga taatgagatg tatgaaaatt gaattttata
      301 ttaattttta tataatttag gtggcaacgc ggataatctc cgtcctatta gtttaggacg
      361 gagtttttta ttttactgaa aatttaaaag atagaaggtg gtgagaaaga tgttatgaat
      421 ttcaaaagct aaagatgatg atatagtagg ttggcaaaaa aataaattga aaattaaaaa
      481 aataggagga agtacaaaaa atgaaaacaa atacaaaaaa attaacttta aatgcaatac
      541 ttttagcgat gggattatta atacatcaaa tcacaccagc tataggatta ccaatgaagc
      601 cagatgtacc actagcaatg ttatttgtaa tattggtttt aaatagagat gattataaaa
      661 cttgtttgat agcgggtata gtaacaggaa tatttacagc acttacatca agttttccgg
      721 gaggtcaaat tcctaatgta atagataaaa ctcttacagc aaatatagta tttttattaa
      781 tgaatattag ttataatatg ccttttataa aaaaattagc taagaaaaca caagatactg
      841 tagtagttgc aataataatg ccaataggaa ctcttgtaag tggaactatc tttttattag
      901 cagctcaagc tatagttggt cttccagggg cttcatttac agctttattc ttagcagtag
      961 tattaccagc tgttttaata aatctagttg caggaatatt tttattcaag attgttagtc
     1021 tatcaataag aagagttagt tatcaagctt aaacacgaaa taatatttta ctttataaag
     1081 cattataata actaaataac ataagaataa agtagattat aacta
//
LOCUS       DAMJWS010000212         1125 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMJWS010000212 DAMJWS010000000
VERSION     DAMJWS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.218"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(205..1074)
                     /locus_tag="PC399_003802"
     CDS             complement(205..1074)
                     /locus_tag="PC399_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2594555.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          421 a          174 c          108 g          422 t
ORIGIN      
        1 tacatcaaaa aataaactaa aaaatatcga aaaataattt aaaagacatc aaaactaaat
       61 cttctaatca gcaattaaaa gtctattttc ttagtttaaa aaataaagat agattttttc
      121 aaaacactgg ataaataggt aaaaaaataa acagttagta aaataaattt ttactaactg
      181 tttattttta ttgtttaata tgtcctataa atcctctaag taatactttc cttgcaaaaa
      241 ttctgattct aaacctagat ttttaaaata atcgaccatg cccatacacg caaatttttc
      301 tgtagaataa tgagtagcac ctataacatt aattaaatta tcttttgcta tttgatggaa
      361 ctctaatact ggttcaaaat gttttaaagg cttagtaaac ccagtaatat atagatttat
      421 accaagttca gcaacttctc ttgctacaaa tggataactt cctccccctg ctgcaatagc
      481 aactaaacca tcttttaata cagaatctcc ataatcatac aatttcacat catgaccaac
      541 agatttttta attaattcct taatctcttc tattgatttt agtgatgttt tacatataac
      601 tcctacttca attttatcgt catatttaca aaatggtttg actatttcta aacccatagc
      661 ttttgcaaaa ctaacagaag ttgaatagtc actatagttg tctagtggag aatgtaatac
      721 ataaaaagaa attctacgat ttttcatctc ttccatataa gatagtggta tgtcgtgaaa
      781 tggaaaacct tcatctgatg ctatgtatcc cattgcatga tgtgaaaaaa ttaaaatatc
      841 tttttcatct ttatctataa cttgcttaat tatttcttta tcaggaaaag tagttgtgta
      901 tactttatta atattttgag cattatctaa tactaatcct atgtattttt ttctaaattc
      961 tggagtaata aaatcatttt caaaattcat aaaactccaa tcatcattaa tatttttaat
     1021 attaaattct aaatctaatt ttttataaag ttcaattgcc ttaataataa ccactccttt
     1081 ataataatat tgaattacta catattaata tagaaatttc cgata
//
LOCUS       DAMJWS010000213         1109 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMJWS010000213 DAMJWS010000000
VERSION     DAMJWS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.219"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(233..724)
                     /locus_tag="PC399_003803"
     CDS             complement(233..724)
                     /locus_tag="PC399_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF2594556.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          424 a          161 c           98 g          426 t
ORIGIN      
        1 agccttttaa gataaataaa aatatctgca actatatatt gcaaaaattt aaaactaatt
       61 tatatatgct aatataaata tttataaaga cataagacaa atttacctac agatttaaat
      121 ttaacttata tattttaaaa ggctaatata atatgtattt tcttctatac aatcaaaagg
      181 cttcatatct aagatatgtg atgacactaa tattcacaat ttttagtaat tatcacatta
      241 taaaaaatat tacagctagt ccatcattag atgcttcttg tataaacttt ttaagctcaa
      301 ttagatttgc ccatacatat tcaaaaaaat catcttcact ctcatcattc ataacaggat
      361 aaatattatt ttctaacatc tcattaatat taaatctttt tctaaatgct tgttcttcca
      421 aactgttcat tgcttttgat agttttttta catcatttgc tgtaatcaac atgacaggaa
      481 attctccatc aatttccata agtatgtttc cacttagtac taatttgcta aatatattat
      541 catcatctcc accaaaagga caacctgtca aagtaaagtg aatggcatgc catgctttat
      601 caatattgat agtactattt tcgttggcat cttcaaaaac aaaatcttgt aaattttctt
      661 ctgcttgctg taatttaaaa acattctctt cacttatttt agtataacat cctatcattc
      721 ccataattac attcctccta agtagcgttt ttttaattgt aacttataat taaacttagt
      781 tacaataaaa tatattgaga aaagtattat ttcatcttaa atttaaatac ctatattatc
      841 aactaaaatt ctgtgtttat agcgtaagta ttattttttc ctaaaaatta aatgaatcag
      901 aggttctata ggcaatattg actataaata agaactatta ctttttaaaa aaaatttaag
      961 aaattatact gtagaatcat gctctactta ttagctttaa ataaaaaata aatcatgttc
     1021 tcaaattaaa taataaataa caataaattt aaattttaaa ctataagagt ttctgaagtg
     1081 ttaataagca taccaataat tatatgtgt
//
LOCUS       DAMJWS010000214         1090 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMJWS010000214 DAMJWS010000000
VERSION     DAMJWS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.220"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            261..941
                     /locus_tag="PC399_003804"
     CDS             261..941
                     /locus_tag="PC399_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2594557.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKVRSITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          479 a           69 c          175 g          367 t
ORIGIN      
        1 gcttatcttt atacagtaaa taaaaaatca aatgaaatac caaataaagt gtatatctta
       61 aaacaattta ctagagatac aaggatgcta tgtgaatttt gaagtgtgca gtttagataa
      121 taaatatata taaataataa agaaaatgga tttatagtat ttaaaatttc ttataagggt
      181 aaaaaatgct attagaaaat gtactaaaaa tatttttcca attctttata gaaaaataat
      241 gatatttagg agagattgta atgcaaattg atgtattgat aattgatgga gataagaata
      301 gttctcaaga gctgaaaaat tttttagaag gaaaggggat aaatgtctat ttagcatata
      361 attgtaaaga tgctattata aaaatattta gcaagaaatt tgacttaata tttttagaga
      421 taattttagt tgatggtgat ggttgggatt tttgtaaaaa agttagaagt ataactactt
      481 gcccaattat tcatataact tatataaatg aaaatcaaag tattttaaat gctttaaatt
      541 ctggaggaga tgattattta ataaagcctt taaatttaga aatattatat gcgaaagtga
      601 aagctatttt aagaagaaga gatttatttt taaataataa taaaaataat ataaaactag
      661 aagattataa tagagctagt ggagtaataa agttagaaaa taaaatgata aagttaactc
      721 caacagaaaa tagattatta aattacttta tagagaatgc tgaaaagact ctaactatta
      781 aggagatata tgaacatgta tggatgaatg agtatttaga tgataattat agtgtagtgg
      841 ttgctgtgag tggattaaga aagaaaatag agaaagatta taaaaatcct caaaaaatta
      901 taacgataag agaagtaggg tattacttta acaagatata aagctttata aacagaatga
      961 atgataaggc atagataata tgttagtata ttacaagagt ttttgttcta acgatatatt
     1021 tgtaaaatta gatttaattt acttaattaa attatagcag tagaataaat ataaaaaatg
     1081 tctgaaatat
//
LOCUS       DAMJWS010000215         1060 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMJWS010000215 DAMJWS010000000
VERSION     DAMJWS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.221"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            522..>1060
                     /locus_tag="PC399_003805"
     CDS             522..>1060
                     /locus_tag="PC399_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594558.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMIS"
BASE COUNT          356 a          129 c          152 g          423 t
ORIGIN      
        1 ataaaaaaac aataattaag aatataacta tattctacat gtaaaaattt ttttatttaa
       61 actcaatttt ctgataattt gataattttt caaaatttat agttgacttt tattttttac
      121 tgtattaaac taattactat acaaaacaga aaacaaaatt ttcaaaatta ttaaattaaa
      181 aggagaggta aatatgaaga ctttattaaa caaacaagta cgatttttta gtagccaatt
      241 tagttagggt ttgtacctaa gttaagacaa attgtcataa catagataca agccccttag
      301 gattatgatt tttaaggatt gtatctatgt tggctatagt aattgttgtt aaagttttta
      361 ttttatcatc ataatataga gccacatgga ttatactgtg tggcttattc gaatttttgc
      421 catacagtta tatatactgt gtggtttttt tatatcaaaa tcatttttta aaaccaaatt
      481 ttaaaaacca tttaaaatat tttcaaaggg gtgttttatt tatgtcaggt attatatctg
      541 taatgacgca aagtttaatt ttatcgatta tggcactggg ggtttatata acttacaaaa
      601 tcttagattt tccagatatg tctgctgatg gtagctatac aatgggtgct tctattgtag
      661 cattttctct tactaatggt atttctcctg tagtagcaac tttaatggct atcttatgtg
      721 gttgtactgc tggtcttgtt acagggattc ttcacattaa gtttaagata tctaatttac
      781 tttctggtat tttagttatg ggaatgctat actctatcaa tttaagaata atgggaaaat
      841 caaatattcc tctatttagc tttaaacact tattcaatgg ggaaatatct cctatagttt
      901 tagccttagc ttttgtattc atctgtaaag tccttttaga tttattccta aaaacaggtc
      961 ttggatacac tttaaaaggt gttggtgata actctcaaat gattaaatct ctaggtataa
     1021 atataggttc tataaaaata ctaggactta tgatttcaaa
//
LOCUS       DAMJWS010000216         1052 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMJWS010000216 DAMJWS010000000
VERSION     DAMJWS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.222"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            118..870
                     /gene="cwlD"
                     /locus_tag="PC399_003806"
     CDS             118..870
                     /gene="cwlD"
                     /locus_tag="PC399_003806"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF2594559.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          468 a          109 c          161 g          314 t
ORIGIN      
        1 gataaacata gtgaattaaa taggggcaga aatataatat cattacataa taatatatta
       61 ataccaataa ataaagatat atcaatatta aaaaaattta aatgtttgta ctaatatatg
      121 gaatattata gtgtttttat aagtatacat atacagggtg atgttgtgag aaagtacata
      181 aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat atttgaaatt
      241 aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac tataatttta
      301 gatgctggtc atggaggcat tgatccaggt gcattaaata aggataagag tacatctgaa
      361 aaggatatta acctagcaat aacacttaag cttagagagc ttatagaatc aagtggtggc
      421 cttgtaatat taactcgaga agatgacagt agcctttata aagaagaaaa taataaaact
      481 acaagacaaa aatataatga gaatttgaaa aatagaaaag aaataatatc taattctaat
      541 gccaatatgt ttgtctctat acatcttaat gcatttgaac aatcaaaata ctatggagct
      601 caaacttttt atcctaaaga caaacaagac agtaaagaat tatcgaagtg tattcaagaa
      661 gaacttaaaa gagtagtaga taagacaaac aacagagaag tcaaaccaag ggatgatata
      721 tatcttttga aagaaaataa tattccatca gtactgatag aatgtggttt tttatcaaat
      781 gaaaaagagt gtaaactctt aactgatgaa acatatcaag aaaaaatagc atgggcaatc
      841 tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc taaatatgta
      901 aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat tttgacaaaa
      961 aaatatttaa aataaaagtt aaaaagttgt tgacttagaa taatatagat gatattatat
     1021 atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAMJWS010000217         1002 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMJWS010000217 DAMJWS010000000
VERSION     DAMJWS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.223"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            35..916
                     /locus_tag="PC399_003807"
     CDS             35..916
                     /locus_tag="PC399_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF2594560.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSIKDVEKFINFFSAIPAKDTDDTIKISEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
BASE COUNT          460 a          104 c          122 g          316 t
ORIGIN      
        1 gtcgacacaa taaatttaaa aaggagaatt aaatatgaaa aaaagattaa taataatgat
       61 attgtctgtt gtcttagtac tatcaagtat acttacgata tttgcatatt caaatattaa
      121 atataataat aataaattga tatattctaa tatgatagat aaaaaaactc aaaatagtgt
      181 aaaggaaatc ctaaaagaaa ataaaattaa tgaaaaagac atagatacat ttattaaagc
      241 agtcaacaat tataataaat tgcaagttaa aatacttcaa aataatataa atatatctaa
      301 atctggatat tcttcaatca atgcaaaaca agttccatat aacctagaaa aattgcaaga
      361 taattgggtg aaaaaattcc ctgattatat ggatgtaaat tgtagaataa ctgcttttag
      421 actttttaaa gattttataa attcaaataa gaaatttact ggagactcta ttgatttgaa
      481 tgtcgacttg gacactataa tgaataataa agatgctaaa tttagcatca aagatgtaga
      541 aaaatttatc aacttctttt ctgcaatacc agctaaagat acagatgata caatcaaaat
      601 ttcagaacaa ataaaaaatg aatggaaaaa aagaaaaata tcttttaaaa ataataaaaa
      661 tatatctatt ataaatggat ttcttcgtta tccagaaact aaaaacgtat ttataggtca
      721 tacaggcata tgtattaaaa ctaaaaatgg cattctattt ttagaaaaat acggtgttac
      781 atcaccttat caagttacta aatttaaaaa taaagaagat gtaaaaaatt atatgtttaa
      841 tagattgaag atgtcagaag gtgaaattga attacctgac cctataataa tggaaaacga
      901 taaacttatg aaataataac tttaaacgca aaataatatt tataattact aagtcaatat
      961 aatagagaag gtctatagat ttgtgtaaat acaagcttat ct
//
LOCUS       DAMJWS010000218          971 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMJWS010000218 DAMJWS010000000
VERSION     DAMJWS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.224"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(241..708)
                     /locus_tag="PC399_003808"
     CDS             complement(241..708)
                     /locus_tag="PC399_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2594561.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDYFDTFVERLSTMRDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          372 a          170 c           64 g          365 t
ORIGIN      
        1 aattatcttt tataagctaa tacttatatt tttctaaatt taaatcatcc aataaatatt
       61 aaattctatt ataatatgta aggaaaatca tgcacttttt aaaaatccaa ataaaattat
      121 aacaaatatt atattctgta tcttacaaaa acaaaaactg taagtaaatt tgtttccata
      181 caagtttact tacagccctt acttctatac aaaatatagt tttactttaa tataatccat
      241 ctattctaaa actgattcaa tatattcgac agttacatcc attcttttag ctacctcatc
      301 tattgaaaga ccactatcta aaaaacgtct acacacagat gacatagttt ctccaacctc
      361 aataatatgc ccatctaaat cataaaatct aattgctctt tgaccccacc tatgctctat
      421 agctagatga acataatcta tatctctcat tgtactcaat ctttcaacaa aagtatcaaa
      481 ataatcttcc tcaaaataca gctcagcatc atttccattg aacttaactt cactgtcact
      541 cttattaata aactctaacc aaagcttttt ttcttgtaat gatatattcc cttctaatac
      601 aacattttct ccaaaatcac agattacctt taaccccaaa acatcataat aaaacttttt
      661 tgatttctca atatcttcaa ctactattaa aacatttaaa aacttcatat tacctcctta
      721 atatacacat actttattta taatatataa ttagaattaa actataaagg tttcaataag
      781 gtctagtata aagaaaaaaa ttataagtct tattgaaata gttaagttaa ttgtatcata
      841 taatataaat tttaatcttg taaaaaacag acacattcat acttttaggt ttaaatctat
      901 atgtattctt attaattttg ctaaaactgt tatttttgta gactatttta gattactgaa
      961 atatctatgt t
//
LOCUS       DAMJWS010000219          744 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMJWS010000219 DAMJWS010000000
VERSION     DAMJWS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.225"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(22..735)
                     /locus_tag="PC399_003809"
     CDS             complement(22..735)
                     /locus_tag="PC399_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2594562.1"
                     /translation="MINIIICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLL
                     KKYPNNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRY
                     LEKPLKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHT
                     EKGIYTFKSSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSR
                     YKIKLLREKLNSCLVDVII"
BASE COUNT          261 a          109 c           52 g          322 t
ORIGIN      
        1 atataaaggc aaaaatttac ctcaaattat tacatctacc aaacaactat ttaacttttc
       61 tctcaataat tttatcttat aacgacttac aattattgaa ttttgattta taaatacaat
      121 attattttct atatctagca tatttacctt cattaaattt ataaggtagc ttttatgaca
      181 tctaaaaaaa ttatgttctt ctagatttga ttctatttct ttcatactac tcttaaaagt
      241 atatattcct ttttcagtat gtatagttaa atcatttttt aaaacttcta aatataaaat
      301 atcatctacc tttatgaaat atatcttgca tttacatttt atagataaaa tagcttcatt
      361 tttgtagcat tcttttaaat atgatgatat tgcttcttta aatagagagt attttaaggg
      421 cttctctaaa tatctataaa ctcctatctc ataacctttc tttatgtatt tatttgatga
      481 ggatacaaat ataattctta cactttcatc aaattttttg atttcttttg aaacattaaa
      541 tccattttca tcatttaagt ctatatccat aaatatgata tcaacattat taggatactt
      601 ttttaataaa tcgcctccat tttcaaactc tttgatattg tattcaaaat tgtaattata
      661 aaaaaaggta tttatataat catttagaat taccctctct gattttttac cttcgcaaat
      721 aataatatta atcatattac accc
//
LOCUS       DAMJWS010000220          741 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMJWS010000220 DAMJWS010000000
VERSION     DAMJWS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.226"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     regulatory      20..113
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            196..681
                     /locus_tag="PC399_003810"
     CDS             196..681
                     /locus_tag="PC399_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2594563.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          325 a           64 c          122 g          230 t
ORIGIN      
        1 tgttcaaatt atgttattat tcagatatag gcaatattag ataaatctaa tgacgcaaaa
       61 ctatagggac taaaatcttt atgtaataaa ggtcatgtca gccagttgcc aaaaagataa
      121 taattctttt tgttaaaagt atttttagag ttatatattt taataactta atatttggca
      181 agaggagggc ttaagatgat atctgaatta atgttaaaaa attttcgcaa atctagcatc
      241 aattttaaga ctaaaattaa attgaattta agagaaaaac cagacataaa tagtttgaaa
      301 ttaaaaagta taccagaagg taaaattgtt aagttaaagt gtgtagatgg aatatgggca
      361 gaagttgaga gtaattatga caaaggttgg ttactttata aatatttaga aagattaagt
      421 aatgctaaaa acaatagtaa tgatacaata aaaaatagaa aaaagaatta cataggaaat
      481 ataagaacaa atggattaag tttagaactt agaaatgata gaacattaga atctaaagtt
      541 ataactacaa tccctgatgg ttttaaagtt gaagtatgtt atagcgttgg aaaatgggca
      601 agagttaata tcaataaaaa tgaaaaacga tattctggat atgtatataa tcaatacata
      661 gaagagcatt taagtttata atacgatact agcaagcttt aaatatatta ataaatcgca
      721 aaaaagaatc ccattaatgg g
//
LOCUS       DAMJWS010000221          726 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMJWS010000221 DAMJWS010000000
VERSION     DAMJWS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.227"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(301..699)
                     /locus_tag="PC399_003811"
     CDS             complement(301..699)
                     /locus_tag="PC399_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594564.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILFIIVIFLVIVLVVLIFIKRKKKKFY"
BASE COUNT          236 a           96 c           60 g          334 t
ORIGIN      
        1 atacaaaatt ctatgaataa ttactttaat ggtctaatag tctgctcttt ggctctaatt
       61 cttcttatat taattttgtg aataactgtt gatgtaattt tatattttga ttcatgagga
      121 attaaattct tcaaagttaa ttaagtacat ttatcaataa atttacttaa ttaaccatca
      181 tttaatactc attcctgtat ttatatgcct cctttgaaac agaatattac tccattttaa
      241 ttacatcata ttattaaata gataccaatg attaaaaaaa tgatattctt ttaattattt
      301 ttagtaaaat tttttcttct ttcttttaat aaatattagc actactaaaa ctataaccaa
      361 aaatatgact attatgaaca atattaattt tgttaggata ttatgtcttc ttgcgttttg
      421 tcttttatat ttatttatag tttgatatcc atacttttta gttcttatac ttcgtttttt
      481 caactttact ttacttttat tatatttcct acttttatgt ctacttttag atttaatagt
      541 tttaaattta gtattatcat atgtataatt ctcttttatt gaagaaatgt atacactaga
      601 ctcaataata gcttctgctt ctacttgtaa tggatttata atgctgatta taagtagaaa
      661 agatatagct atcccaatca attttttctt cttattcata ttttccccct cataaaaatt
      721 tttaac
//
LOCUS       DAMJWS010000222          680 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMJWS010000222 DAMJWS010000000
VERSION     DAMJWS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.228"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            90..>680
                     /locus_tag="PC399_003812"
     CDS             90..>680
                     /locus_tag="PC399_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2594565.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          276 a           70 c          149 g          185 t
ORIGIN      
        1 atagattaag tgaaatgcta ataaattaaa aaagtgaaat atcaaaataa aacgtcaaca
       61 aactaaaaat tatatggggg aatatcaaaa tgttaaatga aacaagaaaa aaacaattaa
      121 ctgaagtatg ccaagactta ataagaaatg ctagttattc aggtcaagaa gaaaacgtag
      181 taaaagctat agaagaaaat tttgctttac taggttttga tagttggtca agagatagat
      241 atggaaatat agtaggatgt ataaaaggaa ataaaccagg taaaaaaata ttatttgacg
      301 ggcatataga tacagttcca gttccagatg caagtaaatg gagtgttcct ccttttgaag
      361 cgagaatagt agatgggaaa atatatggta gaggaacttc tgatatgaag gggcaagtaa
      421 gtgctatgat ggctgctgtt tcatactttg cagaagatac taacaaagac tttgaaggtg
      481 aattgtatgt agcaggtgtt gttcatgaag aaatatttga aggtgtttca gctagagaga
      541 taagtaaggc tgttcaacct gattatgttg taataggtga atcttcagaa ttaaacttaa
      601 agataggtca aagaggtaga ggagaaatag tagtagaaac ttttggtaaa ccagctcact
      661 ctgctaatcc agaaaaaggt
//
LOCUS       DAMJWS010000223          676 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMJWS010000223 DAMJWS010000000
VERSION     DAMJWS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.229"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            197..>676
                     /locus_tag="PC399_003813"
     CDS             197..>676
                     /locus_tag="PC399_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2594566.1"
                     /translation="MYNVIDLFAGAGGLSLGFEMTKKFNMVAFVEKNDNAAKTYLENH
                     PSVKRYCDIKRLDFQDILNSVDKIDVVIGGPPCQGFSNANRQKRKIINGNNELVKLYV
                     DAIDKLKPNVFVMENVKTISSNKHSFYLTKKDKNHIINNLKLNIYNKDSVLYDRTEYI
                     "
BASE COUNT          293 a           50 c          108 g          225 t
ORIGIN      
        1 ataaaaaata tattatgtta tttatttgaa tagattatcc agaagacata aataagaaaa
       61 ataatgtgta agtaaaaata ttagaattta ttaaaagcta ttacaagtat aagaaatttg
      121 aaatgattat tcaaaaattg acatgtgaaa aaaaatatga tatgctgcaa taaaagttaa
      181 tgtgaggtat gtcagaatgt ataatgttat agatcttttt gcaggtgcag gtggtttgag
      241 cttaggattt gaaatgacta aaaaatttaa tatggtggct tttgtagaaa aaaatgataa
      301 cgcagcaaaa acataccttg aaaatcatcc gagtgttaag cgttattgtg atattaaaag
      361 attagatttt caagatatat taaacagtgt cgataagata gatgttgtta taggaggacc
      421 gccatgtcaa ggtttttcta atgctaatag acaaaaaaga aaaattataa atggaaataa
      481 tgaattagtt aagctatatg ttgatgctat tgataaatta aagccaaatg tatttgtaat
      541 ggaaaatgtc aaaactatat cttcaaataa gcattcattt tatttgacta aaaaagataa
      601 aaatcatata ataaataatt taaaattaaa tatttataat aaagatagtg ttttatatga
      661 tagaactgag tatata
//
LOCUS       DAMJWS010000224          651 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMJWS010000224 DAMJWS010000000
VERSION     DAMJWS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.230"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..372
                     /locus_tag="PC399_003814"
     CDS             <1..372
                     /locus_tag="PC399_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2594567.1"
                     /translation="DFLQKLSTKLIKENDIICIEDLQVKNMIKNHKLSRLISDVSWSE
                     FIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDR
                     DINASINILKEGLRLITIQNK"
BASE COUNT          270 a           74 c          101 g          206 t
ORIGIN      
        1 gattttctac aaaaactatc tactaaatta ataaaagaga atgatattat ttgcatagaa
       61 gatttacaag taaagaatat gattaaaaat cataaactat ctcgcttaat ttcagatgta
      121 tcatggtcag aatttattcg tcaattagaa tacaaggcta attggtatgg tagacaaatt
      181 gtaaaggtag gtaaattctt tgcaagttca caaatatgca ataaatgtgg gtataaaaac
      241 gaggaagtga aggacttaaa tataagagaa tggatttgtc ctagttgtaa tgaaactcac
      301 gatagagata taaatgcaag tataaatata ttaaaagaag gattaagact aataacaatt
      361 caaaataaat aacttatgta agaaccgtag gaactacggg gattgcctgt ggagaattag
      421 taagacatat tttagtatgc aaaattctat gaagcaggaa ccctgtgact ttagtcatgg
      481 gaggttcagt ttagcttaaa acttacttat ctatttcagt ttctattaga attattatta
      541 gactcattaa aataaaagca ctaatataac taataatata tgttaaattc attaactcta
      601 cataaagttt tacaatctaa atgaaataca ataatctgca taaaattcaa t
//
LOCUS       DAMJWS010000225          636 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMJWS010000225 DAMJWS010000000
VERSION     DAMJWS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.231"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..634)
                     /locus_tag="PC399_003815"
     rRNA            complement(<1..634)
                     /locus_tag="PC399_003815"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          140 a          175 c          117 g          204 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccag
//
LOCUS       DAMJWS010000226          611 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMJWS010000226 DAMJWS010000000
VERSION     DAMJWS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.232"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>611
                     /locus_tag="PC399_003816"
     rRNA            <1..>611
                     /locus_tag="PC399_003816"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          126 c          179 g          134 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg c
//
LOCUS       DAMJWS010000227          597 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMJWS010000227 DAMJWS010000000
VERSION     DAMJWS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.233"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>597
                     /locus_tag="PC399_003817"
     CDS             <1..>597
                     /locus_tag="PC399_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2594568.1"
                     /translation="ITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVFLIISS
                     LYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVATV
                     SNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIPMIANL
                     LSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISI"
BASE COUNT          208 a           55 c           78 g          256 t
ORIGIN      
        1 ttataacaat tgtttcttct attatagaaa tatggtcatg taaaaaagtt tttgattata
       61 ctagtcagtc aaagaccgac ttaaaaaaac taaatatgta ttttctcttt tctgtttttc
      121 tcattatttc ttctttatat ataaatatag gagctaatag tagaatcttg atatgtatat
      181 ttgtaatgtt cgttttctat aagtctaact ataaggttaa tatatataaa tgtctggtaa
      241 tctcattttt ttattggtta atattaatag gtataaatgg attaagtatg ttattagtag
      301 caacagtaag caatttggat aatatgaatg ttttacttag tggcagttta tatagatatg
      361 aagcaatatt cttgggaaag ataatgttag gtatattact ttatgtttat aaggcttctt
      421 ttaaaaattt taaaataagt aaaaaagaga tatacatatg tattcctatg atagcaaatt
      481 tattgtcatt ttttgtactt tatgattata ttattaatct tgtaaaaatc tctcctgaaa
      541 gaagaactgc ttttttagta atagttttac tattaatatt atctaacatt tcaatct
//
LOCUS       DAMJWS010000228          582 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMJWS010000228 DAMJWS010000000
VERSION     DAMJWS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.234"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
BASE COUNT          233 a           44 c           72 g          233 t
ORIGIN      
        1 cactaaaaat aagaatgtat aaggtggtaa atttatataa aaaaggatat gagttgtaaa
       61 atattaaata atattgtact aattggaata attttaactt tattatagat tattttaata
      121 ctattagtac tgatggtgta ttttaaaatt ggtataagca ttacaaatac gaatatgcca
      181 actgtaatat aagtagctat atacatatgt gctgtcccat atattgtatc attgtttata
      241 ttaaaaagat tatgtaaatt ggtatcaatc aataaatcat tttcagaaga aatacctaaa
      301 aacttaaaaa atacctatat gtgtatttag ttagatttga gatttttaca tttaatttta
      361 tagtgacaat attatattat ttttatgatg tctatttata tgcaattact ataatatcag
      421 aacttatagt atcatttgtt tcattatcaa taaggttttt tactttagtt ttgtcaattt
      481 tattaaaaaa agttattgat ataaaagata agaaggataa aactatatga cggatttcaa
      541 acataagagt ggatatttat ataatagata ttttatagtt aa
//
LOCUS       DAMJWS010000229          577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMJWS010000229 DAMJWS010000000
VERSION     DAMJWS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.235"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>577
                     /locus_tag="PC399_003818"
     rRNA            <1..>577
                     /locus_tag="PC399_003818"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAMJWS010000230          559 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMJWS010000230 DAMJWS010000000
VERSION     DAMJWS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.236"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            163..270
                     /locus_tag="PC399_003819"
     CDS             163..270
                     /locus_tag="PC399_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2594569.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            272..>559
                     /locus_tag="PC399_003820"
     CDS             272..>559
                     /locus_tag="PC399_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2594570.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEK"
BASE COUNT          242 a           51 c           88 g          178 t
ORIGIN      
        1 ttacggttct tatatatgtt atttaaaaat aaatataaaa aaacttcaaa atacttgttt
       61 taagtaattg tatattttaa tattatgata ggttatgaaa aatggaagtt actcatggta
      121 gaggatatgc atatagtatg tcaaatatag acagccagta ttatggggag acatctttgg
      181 aatcctagtt atttcgttgc tactgtatca gaaaatacag aggagcagat aagaaaatat
      241 attaataatc agaaaaaaca agtaaagtga ggtggtagtg gtggaaaaag cttataagtt
      301 tagaatgtat ccaaataaaa aacaacaaga gttaattaac aaaacttttg gatgttgtag
      361 atttgtatat aataagtatc ttgctaaaag aatagatgtt tataaaaaca ataaagaaac
      421 ttttacttat aaacagtgta gttctgattt aaccaattta aaaaaagaat taaaatggct
      481 taaggaacct gacaaatttt cattacaaaa cgccttaaaa gatttagata atgcatataa
      541 aaaattcttt aaagaaaaa
//
LOCUS       DAMJWS010000231          479 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMJWS010000231 DAMJWS010000000
VERSION     DAMJWS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.237"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>479
                     /gene="tuf"
                     /locus_tag="PC399_003821"
     CDS             <1..>479
                     /gene="tuf"
                     /locus_tag="PC399_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2594571.1"
                     /translation="ATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVE
                     MEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTD
                     KPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRK"
BASE COUNT          197 a           60 c          116 g          106 t
ORIGIN      
        1 gcaacagatg gaccaatgcc acaaacaaga gagcatatac tattatcaag acaagttgga
       61 gtaccatata tagtagtatt cttaaacaaa tgtgacatgg tagatgatga agagttatta
      121 gagttagtag agatggaagt aagagattta ttaacagaat atgatttccc aggagatgac
      181 actccaatag taagaggttc agcattaatg gcattagaag atccaaagag tgagtgggga
      241 gataagatag tagaattatt cgagcaaata gatgagtata taccagctcc agagagagat
      301 acagataaac cattcttaat gccagtagag gacgtattct caatcacagg aagaggaaca
      361 gttgcaacag gaagagtgga aagaggagta ctaaaagtac aagacgaagt agaattagta
      421 ggattaacag aagcaccaag aaaagtagta gtaacaggag tagagatgtt cagaaaatt
//
LOCUS       DAMJWS010000232          446 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMJWS010000232 DAMJWS010000000
VERSION     DAMJWS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.238"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(5..298)
                     /locus_tag="PC399_003822"
     CDS             complement(5..298)
                     /locus_tag="PC399_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594572.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT          174 a           79 c           26 g          167 t
ORIGIN      
        1 tactttaaag attcccatct ttatcatatt ttattcttat aagaaaatat aatcctatta
       61 ctactaactc tatcactaaa aatactccat aggtaagaat tgaatcaata ctatctaacc
      121 catttctaaa aaaaattaat gctatacctg ctgctataat ccctatagca ttttttctta
      181 ctgcattttg cccaactcca taaaaacctg gcatttttcc ttttatttcg acagcagtta
      241 aaattatccc aaacactata aataatactc caataagcat cataatcata ctaatcatct
      301 aattacctcc ttcacttaag gaaatttcaa tttctaaaat ctaaaataaa taatatttat
      361 tttttataat aatattgtat atccaattaa ctattttgga aattagtacc aataaataat
      421 gtttaaatat tgaaataata tattaa
//
LOCUS       DAMJWS010000233          405 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMJWS010000233 DAMJWS010000000
VERSION     DAMJWS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.239"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..215
                     /locus_tag="PC399_003823"
     CDS             <1..215
                     /locus_tag="PC399_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2594573.1"
                     /translation="IGSHPSFQLFHDLVTMFNISVDEYFYPAEKVAKSTARRQIETSL
                     DLLSDNELKIIQGTIDGILNSRENKK"
BASE COUNT          125 a           73 c           68 g          139 t
ORIGIN      
        1 atatcggttc acacccaagt tttcaattat tccatgattt agttaccatg ttcaatatat
       61 cggttgatga atatttctat ccagcagaga aagttgcaaa aagcacagct cgccgtcaga
      121 tagaaacatc acttgattta ttgagtgata acgaattaaa aattattcaa ggtacgattg
      181 acggtatctt gaacagcagg gagaataaga agtaatcagc ttcttgtttt ccccgttttc
      241 tttttagcca tatttcttca agacttattt gttgatctgc tttgcaggaa acaaataaga
      301 gccgacaatc tataagttac aataacctac aaattatcgg ctctgcgtct tagcgtctgg
      361 ctctttgata atagttactt ttccatgctt tatattgatt aaagt
//
LOCUS       DAMJWS010000234          399 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMJWS010000234 DAMJWS010000000
VERSION     DAMJWS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.240"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
BASE COUNT          147 a           56 c           24 g          172 t
ORIGIN      
        1 ttagtatcat catttattta atacattatg tattccgttt agatttagtt atccgaaaac
       61 ttagataact agcttgaaat ttctacagat tattacatac tttattgtaa ttatatcata
      121 atgttataat tacatattat caaattacac actatctttt attcatatag atattatcat
      181 ccttgtgtat atattgtcaa agaaaacatt taatttacaa catattttat atcatatcat
      241 ctaaactatt atatacacct tatctaattc acacattaac actattgtac tttttctaaa
      301 aatttttaaa taaaaatgac agctttaatt tattatttta aaactgccat taaactgtaa
      361 atattccctt ttagtataca aaattttacc tttatgtta
//
LOCUS       DAMJWS010000235          391 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMJWS010000235 DAMJWS010000000
VERSION     DAMJWS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.241"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            154..270
                     /gene="rrf"
                     /locus_tag="PC399_003824"
     rRNA            154..270
                     /gene="rrf"
                     /locus_tag="PC399_003824"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            276..364
                     /locus_tag="PC399_003825"
     tRNA            276..364
                     /locus_tag="PC399_003825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Leu,seq:taa)
BASE COUNT          131 a           69 c           83 g          108 t
ORIGIN      
        1 taactctata tttttaaact tgtcaaaact tatggatttt taatagtttt ataaggcaaa
       61 aaatcgaata aaaaaagtat tgacacaata agacaaagat gttattataa tacttgtctt
      121 agaaaaaaag ttaaatgaaa atttagacaa taatctggtt ataatagcag agaggataca
      181 cctgttccca ttccgaacac agaagttaag ctctctagcg ctgatggtac ttggtgggaa
      241 actgcctggg agagtaggac atagccaggt gatgtgccga agtggcggaa ctggcagacg
      301 cacaggactt aaaatcctgc gggacttacc tctcgtaccg gttcgattcc ggtcttcggc
      361 accacaaatt attgtgggcc attagctcag t
//
LOCUS       DAMJWS010000236          381 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMJWS010000236 DAMJWS010000000
VERSION     DAMJWS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.242"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(<1..>381)
                     /locus_tag="PC399_003826"
     rRNA            complement(<1..>381)
                     /locus_tag="PC399_003826"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           89 a          107 c           87 g           98 t
ORIGIN      
        1 tgtactaccc attgtagcac gtgtgtagcc ctaagcataa ggggcatgat gatttgacgt
       61 catccccacc ttcctccagg ttatccctgg cagtctctct agagtgccca acttaatgat
      121 ggcaactaaa gacaagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga
      181 gctgacgaca accatgcacc acctgtcacc aatgtccccg aagggaactc tccgattaag
      241 gagatgtcat tgggatgtca agcttaggta aggttcttcg cgttgcttcg aattaaacca
      301 catgctccgc tacttgtgcg ggtccccgtc aattcctttg agtttcactc ttgcgagcgt
      361 acttcccagg cggagtactt a
//
LOCUS       DAMJWS010000237          373 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMJWS010000237 DAMJWS010000000
VERSION     DAMJWS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.243"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            177..293
                     /gene="rrf"
                     /locus_tag="PC399_003827"
     rRNA            177..293
                     /gene="rrf"
                     /locus_tag="PC399_003827"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            300..371
                     /locus_tag="PC399_003828"
     tRNA            300..371
                     /locus_tag="PC399_003828"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:332..334,aa:Asn,seq:gtt)
BASE COUNT          118 a           61 c           80 g          114 t
ORIGIN      
        1 tttgatgtta ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta
       61 ctaacttcaa ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact
      121 attgacattt aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg
      181 gttataatag caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta
      241 gcgctgatgg tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt
      301 ccaaggtagc tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc
      361 cacccttgga gcc
//
LOCUS       DAMJWS010000238          320 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMJWS010000238 DAMJWS010000000
VERSION     DAMJWS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.244"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
BASE COUNT          123 a           32 c           27 g          138 t
ORIGIN      
        1 aattaatatg gataaatatt ctaaatccaa tacttattaa aaatgaaata aaaagaaaaa
       61 aagtacaaat tattatattt ttctttataa actttggttt aacttattca ttaggtttat
      121 tattgtatta taataaattt attgatccag aaaactttgc atcattcatg atgatattac
      181 ctctatcttc agttgctatt gcaaaatttt atactgaagg attaaataat gatatgtatg
      241 aattttattc actaatcata ctattttttc ttacttattc gttattaatt gttcttaagt
      301 tattaaatct aataaataat
//
LOCUS       DAMJWS010000239          304 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMJWS010000239 DAMJWS010000000
VERSION     DAMJWS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.denovo.245"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            <1..>304
                     /locus_tag="PC399_003829"
     CDS             <1..>304
                     /locus_tag="PC399_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HDF2594574.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPELNAKVSYAVEKADCYTGNTIESER"
BASE COUNT          119 a           44 c           55 g           86 t
ORIGIN      
        1 ctaatgtaat ccaaaagata caacaattag tacctccaac tcatccagtt ttaggtgatg
       61 gaatattagt tttaacagat ataaaatctt ctccatatcc aggagcatca gtagtgccag
      121 attattgcaa agctactttt gatagaagat tattagttgg agaaacaaga gaaggcgtat
      181 tagctccaat acaagcttta ttagatgaaa tgatgaaaga agacccagaa ttaaatgcaa
      241 aagtttctta tgcagttgaa aaagctgatt gctatacagg aaatactata gaaagtgaaa
      301 gatt
//
LOCUS       DAMJWS010000240        22924 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.guided.070, whole genome shotgun sequence.
ACCESSION   DAMJWS010000240 DAMJWS010000000
VERSION     DAMJWS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.guided.070"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            227..844
                     /locus_tag="PC399_003830"
     CDS             227..844
                     /locus_tag="PC399_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2594575.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            952..3483
                     /locus_tag="PC399_003831"
     CDS             952..3483
                     /locus_tag="PC399_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2594576.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3917..4618
                     /locus_tag="PC399_003832"
     CDS             3917..4618
                     /locus_tag="PC399_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2594577.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4668..5750
                     /locus_tag="PC399_003833"
     CDS             4668..5750
                     /locus_tag="PC399_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2594578.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            6011..7111
                     /gene="vanG"
                     /locus_tag="PC399_003834"
     CDS             6011..7111
                     /gene="vanG"
                     /locus_tag="PC399_003834"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2594579.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7108..7914
                     /locus_tag="PC399_003835"
     CDS             7108..7914
                     /locus_tag="PC399_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2594580.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7932..10070
                     /gene="vanT"
                     /locus_tag="PC399_003836"
     CDS             7932..10070
                     /gene="vanT"
                     /locus_tag="PC399_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2594581.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10384..11004
                     /locus_tag="PC399_003837"
     CDS             10384..11004
                     /locus_tag="PC399_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2594582.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11170..11706)
                     /locus_tag="PC399_003838"
     CDS             complement(11170..11706)
                     /locus_tag="PC399_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2594583.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11819..12493)
                     /locus_tag="PC399_003839"
     CDS             complement(11819..12493)
                     /locus_tag="PC399_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2594584.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            12696..14570
                     /gene="asnB"
                     /locus_tag="PC399_003840"
     CDS             12696..14570
                     /gene="asnB"
                     /locus_tag="PC399_003840"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2594585.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(14662..16098)
                     /locus_tag="PC399_003841"
     CDS             complement(14662..16098)
                     /locus_tag="PC399_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2594586.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            16427..17005
                     /locus_tag="PC399_003842"
     CDS             16427..17005
                     /locus_tag="PC399_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2594587.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            17044..17622
                     /locus_tag="PC399_003843"
     CDS             17044..17622
                     /locus_tag="PC399_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2594588.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            17708..18337
                     /locus_tag="PC399_003844"
     CDS             17708..18337
                     /locus_tag="PC399_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2594589.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            18327..21086
                     /locus_tag="PC399_003845"
     CDS             18327..21086
                     /locus_tag="PC399_003845"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2594590.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            21173..22735
                     /locus_tag="PC399_003846"
     CDS             21173..22735
                     /locus_tag="PC399_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2594591.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            22736..>22924
                     /locus_tag="PC399_003847"
     CDS             22736..>22924
                     /locus_tag="PC399_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2594592.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDT"
BASE COUNT         8829 a         2467 c         4020 g         7608 t
ORIGIN      
        1 ttcatgaggc actctttttg tatgaaatca aatatatttt tattatttca acaataaaaa
       61 agaggtctgt ttctattttg agacaatatt atttttatgt agatttaatc ttaatgagta
      121 taaagtttta aaactactaa aataggagga attttaatat aaaagggtaa ataaagggtt
      181 tagagggcat aatatagaat aaattaatta tttaaggagg gtttttatgt gcaaaaaaat
      241 gtatttatta gtggtaggta tacttatagc aggtttatta actgcatgta atgggagtcc
      301 aactaaggat tcagaacaag cacaagataa tacaaaaaag gaggcaaatg tatcaaataa
      361 cgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa caagatttta aagtgccata
      421 cacagatgca ataaacatat ttaaagacaa atacaaagat gctgatatag tagatttaag
      481 tcttgagaga gatttaaata aatttgttta tactgtggaa ggtgtagatg ataataatga
      541 atataagatg aaaattgatg ctaatacaaa agatgtatta gaagataaaa cagaaaagtt
      601 ggattcagaa gatttaaatg gagtcgctag aaaagaaaaa ttagatttta atgacataat
      661 gacacctcaa caagctatgg aaattgcatt aaaagaacaa aatggaatgg ttaaagagtg
      721 gagtttagat aaagatttag atgttacttt ttataaaata agaatagata aagataaaaa
      781 tgaatatgac ataaaagtag attctaaaaa gggaactgta ttgaaggtag aaaaagaaga
      841 ttagaagatt taaactatct ttaaaataat ttcttttttg aataaaattt gtttaaaata
      901 taacaagtag ggtataaaga gactaaataa ctattttagg aggagtacgg tatgtcattg
      961 aaatcgctaa aaataaaaga aaatttatat tgggtaggtt cgcttgaccc tgatttaaga
     1021 gtatttgaca taattatgta tacaccttat ggaactactt ataattcata tgtattaaaa
     1081 ggaacagaaa aaacagtttt atttgagaca gttaaagata agcattttga caattatatt
     1141 gaaagattaa atgatttaaa tatagatttt gaaaaaatag actatattgt tgtaagtcat
     1201 actgaaccag accatgcagg aagcgttgaa aagcttttgg atttagctaa aaatgcaaaa
     1261 gtagtagctt cagaaacagc aattaaatat cttaaggaaa tcgtaaacaa agactttgaa
     1321 tatgttgcag ttacagatgg agatacatta tcaattgggg acaaaacttt agaatttttc
     1381 tctgtaccta tgcttcattg gccagatacc atatacacat atataaaaga ggataaaact
     1441 cttgtaacat gtgattcatt tggtagtcac tatagtaatg ataagatagt aaatacactt
     1501 gatgaagatg aagaaaagga ttacttggat gctttgagat actattatga ttgtataatg
     1561 ggaccattta aaccatctat ggtaactgcc atagaaaaga taaaagattt agatatagat
     1621 actgtatgtc caggtcatgg tccagtatta actgaaaatc ctagaaaaat tatagacctt
     1681 tattacaact ggagtgtaaa tgaacaaata aagttagaaa aagaagttac aatttgttat
     1741 gtttctgcac atggatatac aaagattatg gcagaagcta taaaagcata tattgaaaaa
     1801 aatagcaact ataaagtaaa tttatttgat gtaatagagc ataagcaaga agatattttg
     1861 gcaaaaatag cagtatcaca aggtgtatta tttggaacac caacaatatt aggagatgcg
     1921 ttaaagccta tatgggatat attaatatcg ttaaacccag ttcttcatgg tggaaaagtt
     1981 gcttcagtat ttggttcata tggatggagt ggagaaggta tagaaaatgc tatggagaga
     2041 ataagtcagc ttagaatgac agcagtaaaa ccttttgctg ttaactttaa accatcaaat
     2101 gaagagattg ataaattaca ttcttataca ggtaaattcc tagataagtt aaattctact
     2161 tttggaagta aaaagaaaac taagaaattt aaatgtgtaa tttgtaatga agtttttgaa
     2221 ggtgatagtg ctcctagtgt atgccctgta tgtggagcta aggaagacca atttatagaa
     2281 gttgaagaag atgaagttac ttttagaaaa gatactgatg aatatttcgt aatagtagga
     2341 aatggagcag ctggttttta tgcagcagat gctataagaa aaagaaataa aacttgtaag
     2401 ataactatga tttctaatga agatgagttg acttactata gacctgcact ttctgatggt
     2461 ataaatgaag aacttggttc agatttctac atggaagata aagattggta tgataaaaat
     2521 aatatagtag taatattagg aacgaatgta gataaattag atgaagtgaa taaaactatt
     2581 atagtgaatg atggagctat taaatttgat aagcttgtaa tagctacagg aagtagaaac
     2641 tttataccac caattaaggg tcatgattta gaaaatgtgt ttactcttag aaatataaaa
     2701 gatttatata gtgtaaaaga agcactagaa aaatctaaga aagttgtagt gattggtggc
     2761 ggtttattgg gacttgaagc tgcatgggaa tttagactaa aaggattaga agtagtagta
     2821 gttgaagcca tggatagcat actatcaaaa caacttgata aagaaggcag taaaatatta
     2881 gaacaatgtg taagagatac aggaatagat gtaagattgg gtgtagcagt tgatggtatt
     2941 gaaggtgatg gaaaagctca aaaagttgta tttaaagatg gagatagtgt tgactgtgat
     3001 atggtagtat tttcaattgg tgtaagagca aacactcaaa tggttcagga tacatctgta
     3061 aaaattgaca gaggtattgt tgtagataag acattacaga caaatgttaa agatatatat
     3121 gcttgtggag atgttgcaca agtgggaaat attagtttag caatatggcc atcttcagta
     3181 gaaatgggaa aaatagcagg ggcaaatgca agtggagata atttgacatt tgaaagtgaa
     3241 gtatatccag tttcattaga tgcaatgaat gtaaaagtat ttagtattgg taatatacaa
     3301 aattttgata aagaaatttc atcaaaagat gaaggtcaaa gaatatataa aaaattattt
     3361 atgaaagatg gttcattagt tggagctata cttataaatg atttaagctg tactgttaaa
     3421 ttgataagat taatatctga aaaaggtgat tttgaggata ttatgaaggc tgatatacta
     3481 taatttaaat acattaaaat aaaaaggagt tgtcttaaaa ttgatttttt tctaatcaaa
     3541 ttttaaattc aactcctttt ttgaattttt atataaaata acatggaaat tttagtaatg
     3601 tctgtggtta catacatagg tttaattgtt ttttatatgg attgtctctg gtataataaa
     3661 ttgaattgtg tttgactgat agatattttt attgaaagtt aatacctgtt atgcgataat
     3721 aaggacgtgt taggggagtt tttataataa caattaatct cagtttttgt gttccaatag
     3781 tagcatcaac acattctgat gtagtagttc ttttcttata aaaattagtt acaaatatgc
     3841 ttgtaattat ttgatatgta aatttaccaa tattataaat gatgacctcg ataataagga
     3901 gagtgaaaaa taatctatga acgaaaatat tttgattgtg gatgatgaaa aagaaatagc
     3961 agatttaatt gaagtatatt taaaaaatga aggttttaca gtatacaagt tttatacagg
     4021 taaggaagct ctagaatgta ttgaatctaa aaatttggat atggctattt tagatattat
     4081 gcttcctgat attgatggat ttcatatttg ccagaaaatt cgagaaaaat actattatcc
     4141 tatcattatg ttaacagcta aaattgaaga tatagacaag ataatgggtt taaccatagg
     4201 tgctgatgat tatataacaa agccatttaa ccctttggaa gttgttgcaa gagtaaagac
     4261 acaattaaga agatatatgc gatataataa ttcttatgaa caacagtcga taattgttaa
     4321 tgagtatgat attaaaggat tgattatcaa taaagaaaca cataaatgta gtttatttgg
     4381 gaaagaagta gcattaacac cgattgaatt ttctattctt tggtatttat gtgaacatca
     4441 aggaaaagtg gttccttctg aagaattgtt tgaagctgta tggggagaaa aatatcttga
     4501 taataataac acagttatgg ctcatatagg gcgattacgt gaaaaaattc atgaaccttc
     4561 taaaaacccg aaatttataa aaactgtatg gggagtaggt tatacaattg aaaagtaaaa
     4621 atattaaagt caatcctgac tttactcaag tgagaaataa aatttttatg agaacagctt
     4681 taatggcatt tatagccatt attttcatat ggattttata ttcttttata ttgtatggta
     4741 agttttctat ctggatagta tcattctttg aaaatgcttt tcgtatggaa tataaagctg
     4801 cattgaattt atatcagcag attttccgta atagtatgga gctcttcatg atactagcta
     4861 tggtgattgt gttttttata atattccgtg tttatttaaa ttggtttaca aaatatttta
     4921 gtgaaataaa tcaaggtatt gactctctta tcaaagaaga tgttggagaa gtggctttat
     4981 ctccagagtt attggcaata gaaaagaaaa taaactcaat taaacatata cttgaacaac
     5041 gtaaatttga gacacaaatg gcagaacaac gtaagaatga acttattgtt tatcttgcac
     5101 atgatttaaa aacaccactt acatcagtta tagggtatct gacactactt cgagatgaaa
     5161 gtcagatttc tgaagaactg cgaaaaaaat atttatctat ttcgttggat aaggcagagc
     5221 gtcttgaaga tttaattaat gaattctttg aaattacaag gtttaattta tcaaatatat
     5281 cacttgaata cagtacagta agcttaactc gtatgcttga acaactaaca tatgagttta
     5341 aacctacttt gatgagtaag aatttaaaat atacactgaa tgttacacca aatatgatga
     5401 taaggtgtga tgttaataaa atgcaacgtg tatttgataa cttattacga aatgctgttt
     5461 attatagttt tgaagatact actattgaaa taacagctgt acaggaggat gattatataa
     5521 agcttaaatt tataaatcat agcaatacga ttccagagga aaaacttgag cgtatttttg
     5581 aacaatttta caggttggat acatctcgta attcaaacag tggtagagct gggttgggac
     5641 ttgctatagc taaagaaata gtggagcttc ataacgggac aataacagct catagcgaga
     5701 acaatataat tacctttaag gttattattc cgattgtagg aaatttgtaa gaatttaagt
     5761 ataaaaaaat aggaaattct ttaggataaa taaataaaga gcactcttgt tattgatata
     5821 attactgtat caaaacaaga gtgcttttat tttgatacag taattatatc aatagaaaaa
     5881 tgtgaaaata tattatgtgg aacagatgca attgtaagtg aaggttgatt ggatttgaaa
     5941 tagaaaaagt attttaacat gtttctgtag attgtataaa aataaataaa tcaacaaagg
     6001 agaatcaaag atgaataata aaaaaattgc agttatattt ggtggaaact caacagaata
     6061 tgatgtatca ttacaatcag cttttgcagt acttgagaat ttgaatacgg aaaaatatga
     6121 tattatacca attggaatta caagggaggg taattggtat cattatattg gagattataa
     6181 taatatccag aataacactt ggtttgaaag ggatactgat ataattccag ttattgtttc
     6241 gcagaaccgt gtcaaaaatg gtattattga atttttaatt cctgatgctg tttctcagaa
     6301 tcatattcca aatagtgcta ttgaatcatg tgaaaatgat attaaataca ctaaacttga
     6361 ttttgtgttt cctgtactgc atggaaaaaa tggagaagat ggaacagtgc aggggttatt
     6421 tgaattggca ggtatacagg ttattggatg tggtactctt tcttcagcat tatgtatgga
     6481 caaagacaaa tcacataaac ttgttcattc agaaggtatt aaagtaccaa aatcaatatt
     6541 actaaaatac tcagataaat ttaatgtggc tgaactgtca aaagacttaa cttatccatt
     6601 atttataaaa cctgtgcgtg caggttcatc atttggtatt agtaaaattt ataaaaaaga
     6661 agagttacaa gtagcagtag acttggcttt tgaacatgat aatgaagtaa taattgagga
     6721 aaatattgat ggttttgagg tagggtgtgc agttttagga aacaatgact tacttgttgg
     6781 aagagtagat gaaatagaat tgtcaaaagg attttttgat tatacagaaa aatatacatt
     6841 gaaaagttca aaaattcata tgccagctag aattgatttg gattgtgaga aaagaataca
     6901 agagacagca aaaattattt atagaactct tggatgttct ggatttgcta gagtagatat
     6961 gtttttaact ccatctggtg aaattatttt taatgaagtc aatacaattc ctggctttac
     7021 ttctcatagt cgttatccaa atatgatgaa aggtattgga ctgtcttttg aagaaatatt
     7081 ggacaagata attgaggtgt acataaaatg atgaataata ttttattaga taaaaataaa
     7141 gaagataaaa tgacaaatac tatcttattg actaaagata aagtttatga tgggaatcta
     7201 attctagtaa atgctttttt accagttaaa acatctgaag atatagattt aattcctgta
     7261 gatacaaggt ttccaagtat tcttatgaaa cgtgaagcta caaacatttt acaaaatatt
     7321 ttaaaaagta tatgtggaat aaatgaaata gtacctgtaa gtggatatcg tactgcagag
     7381 gaacaacaag atatatattc aagttctctg cgtgacaatg gaaaagactt tactaaaaag
     7441 tttgtagcac ttccaaatca cagtgaacat cagacagggc ttgctattga tttagggtta
     7501 aagaaagaag ttatagattt tatctgccct gaatttcctt ataatggtat ttgtaatgag
     7561 tttcgtaagg cagcactaca ttatggtttt atagagcgtt atcagtgtaa aaaagaagaa
     7621 ataacaggaa tatctcaaga accatggcat tttcgttatg tgggatatcc gcattctgag
     7681 attatcaata aaatgaatct cgctcttgaa gaatatatag aatatattaa gagattttcg
     7741 tatacaaatt gtttaaaaat agaagataaa gaaaaaatta tagaaatctt ttatgtgcct
     7801 attcatacta aagaagaagt gagaatctct atacctgaac aatgtgtata tcaaatatcg
     7861 ggtaacaata aggatggttt tattgtgaca ttttggagaa atagaaagga ctaagaaatg
     7921 gaggaacaag aatgattagg aataaatctt atgcagggct tgattatttt aggattatag
     7981 ctgcattact tataattgca attcatacat ctccattatt atcatatagt aaaatggctg
     8041 actttgtgtt aactagtata attgctcgta ttgctgtgcc attctttttt atgctatcag
     8101 gcttttttct aatttcaaca tataattata attgtaataa actgaaatct tttctaaaaa
     8161 atgtattatt tatttacggg atagctattg taatttatat tcccttaaat atttacactg
     8221 gatatttttc catgaataat ttagtgccca atattattaa ggacattgta tttgatggga
     8281 ctttatatca tttatggtat cttcctgcat caattattgg tgcaagtatt gcatactttc
     8341 ttgtaaaaaa actaggttta aagtactcac tagtaatcac tattgttatt tatgtaattg
     8401 gtatgtttgg tgatagttat tatggttttt tagaaaaaat accactatta aagaatttat
     8461 atgggtatat ttttgaagta tcagattata cacgaaatgg tatatttttt gcacctgtat
     8521 ttttcatact tggtggaata atatctaata aatctaatta tatgtcaact aaaaatagct
     8581 tgattggatt tcttatatca ttcttactta tgttatgtga gggaatgttt ttacataaac
     8641 taggggtaca gcgtcatgat agcatgtata ttatgttatt gccatgtatg tatttcttat
     8701 ttacagcctt aacatttttg aaaggttctc gagctaggtt attgcgaaca tcatcattaa
     8761 ttatttatat tatacatcct atgatgattg ttgttattcg tatgttttct aaaatattcg
     8821 gaatacaaac tgtcttggtt gacaatagcc ttattcatta tatagtggta agtatagcat
     8881 cttttatagc ttctataatt attatattga ttcttaaacc tctaaaaaaa gacttgaaag
     8941 gagaacataa ggtaaatacc aatagagctt ggattgaaat tgatttaaat aatttggaac
     9001 ataatgtaaa gatgatacaa aaagctatgc cagaagattg tgaattgatg gcagttgtaa
     9061 aagctaatgc ctatggtcat agtgcttttg aagtagcgac ttgtgcaaac caaataggtg
     9121 taaaagcatt tgcagtagca acaatagatg aggggattga tttacgtcga tatggaatac
     9181 taggagaaat attgattctt ggatatacta acccaataag ggcaaaggaa cttcataaat
     9241 acaatcttac acagagtgtt attgacctta atcatgctat ctctttaaac aaacagaaat
     9301 acaacataaa atcacacatt aaaattgata caggtatgca cagactgggt tttgatgtta
     9361 atgatgtgag gtcaatttta aaggcattta aattaaaata cttagatata tgtggcatat
     9421 ttacacactt gtgtgtttca gatagtctga aagatgaaga tgtagacttt acaaataaac
     9481 aaattgaatg tttttataag ttaattgatt ttctgttaaa aaaaggtata aaaattccta
     9541 aagtacatat tcaaagtagt tatggttttt ttaattatcc caatcttaag tgtaattatg
     9601 taagaattgg aattgcatta tacgggatat taagttttcc aaatgatacg actaaattgc
     9661 aacttgattt acgacctgtt ttatccttaa aatcacaagt aattcttatt agaaaaatac
     9721 aaaaagatga tagttttgga tatggaagag tctttattgc aagcagagat agcgtaattg
     9781 ctatattatc tattggctat gccgatggta tacctagaaa tttatcctgt ggaaaaagtt
     9841 acgtaataat aaatggttgt agagcagcaa tagtgggtcg aatttgcatg gaccaacttg
     9901 ctgttgatat tacagatata cctaatgtag aagttggtaa tacggcaata ataataggaa
     9961 gagataattt atctgaatta tctgtttctg aagttgcaaa taactcatgt agtatatcga
    10021 atgaattatt gagcagagta ggaagaaggt taaatgttat aaagaagtaa ctcaattaaa
    10081 tagtaatatt tgttgtactg tgtaatttat tttactctga ctagacattt ttagtaatat
    10141 tatgtctaat atatattttt tataacttac atgacaattt aagttataaa aaggtgatgt
    10201 ttcagatttt tattttgaaa catcaccttt tttgttataa tatataataa aataattata
    10261 ccttaactgt aaacttatat agattattta ctttttaata ttagattcat tttttgagat
    10321 tggtatataa taaacatata gagtatataa taatagttaa agttaaaaaa ggggaaaagc
    10381 aatatgcagt atatacaaga actacttgat ttgactcaaa attattgggt gttagccaca
    10441 ataatcggct tattaagtgc atttatagaa agctttatac cagccttacc acttgttgct
    10501 atagttacag caaatgctgc aatacaagga ttatttattg gatgtttgtt atcatggatt
    10561 ggctcaggac ttgggacaac ttcattattc ttattaataa gcagatttaa tgatagtaag
    10621 ctttttaata agttgagaaa tagtaaaact gaaaaagcaa taagttggat ggataaacag
    10681 ggatttaagt tattatttat agcgtatgcc tgtccattta tgcccggttg tttagttact
    10741 atcgcatcag cttttggtaa aaaagattta aaagattttg tgccagcaat gttggcagga
    10801 aaatttgtaa tgtttatagt tataagttac gtggcaagtg atatagaagg ttttataaca
    10861 agtcctttaa agatagcatc atttatatta ttggtgtttt tgtcatggaa gattggaaat
    10921 agagtaaata aaaatcttga aaatcataat tatgatttcc atcataaaaa acatcacaat
    10981 gataaagatg ataaaatgat ttagcttgta cgaaataaaa cgtatcttaa aatgatttag
    11041 tactaaatta ttttaagata cgttttattt tttgtattaa ggtacaaaat ttttagttct
    11101 tttgtaaact aaaatatata aaacttattt atacttagtt caaaatattg ttatttattt
    11161 tttctacaac tataataaaa tataaaattg atatccaatc aaaaaaataa ctatgaaatt
    11221 taatagctta gatatgaatt tttctgtttt tgagttagtt tttataggta gtaaagaaat
    11281 gtttatagta atgggataaa acaattcaat accttcttta gtaataaggt caagacacaa
    11341 gtgagataga tatcctatta taaacccact ggacaaacat gagaaaacta tattattgtt
    11401 agtgaagatt gtcagagatt taaaaaaact ataaataagt aataaggcaa ttagactgtg
    11461 agtaaaccct ctatgtctaa acctacttcc aaataatttg tatattaaca taaatttttt
    11521 acttatgtaa gaacctctca tatctatatc tggaaataaa gaacctaaat atgcaaaata
    11581 aatataaatt ataattaata ctattttata tgcaatatcg tatttaacta ggtacagatt
    11641 gtttataaag gggagtatca aaagtgcgaa tatataacct cccctttcat gtgtttcttt
    11701 tatcatataa aaatcctcct gaatcgtcac tataaacatt atattggtaa atttagacta
    11761 tcttttatta tattatatag caaacataag tttttttcaa aaggatttta atatttaatt
    11821 aatcttgaga ttttaaattt ttgtaatttt ctaaagcacc attccaattt actacattaa
    11881 accagttgtc aatgtattca tttcttctat tttgatattt taaatagtaa gcatgctccc
    11941 aaacatcaag tcctattata ggagttaggt ttttacttac agggctatcc tgatttggag
    12001 tagtcataat agataatttc ccatctttag tagctacaag ccaagcccaa ccagaaccaa
    12061 agacatctaa agcagatttt tggaactctt gcttaaattt ttcaaaagaa ccaaagtctc
    12121 tatcaatagc ttcttttaaa gattcagaag gtatggtttt ttctggcgtc attatatcaa
    12181 aaaagaattt atgattataa gctccacctg cattatttct tacagttgta gcaatatctt
    12241 taggtaaaga atctaaattt tgcaataatt cacataaaga ataattataa agctcaggat
    12301 atttttcaag agcagcattt aatttatcaa cataagcttg ataatgctta tcatgatgca
    12361 gtttcattgt ttctttatct atataaggtt caagtgcatc atatgcataa ggtaatggtt
    12421 taaccttaaa tttgttattt tcaggtgtaa aagcaaatga agttatgcac tgtgagatta
    12481 taaataaaga cataataact ggtattaata tttttttctt cataaaaatc acctcctaat
    12541 atagtatttc tagaaataaa aaaaatatga aatattaaca gataatttat aagaatttaa
    12601 tagcaatttc attttaaatt gaataagttt taaagccaaa gacaaaacta tgaaatatag
    12661 aataagacta aaggacagaa aggaattttg attatatgga cggcaatatt tcaatttatt
    12721 ataaaggtga tatatctaaa aaagaagata ttaaatttaa aataaaagaa tctgtaaata
    12781 atacaagact ttctagtaga atagagtata aggatgagga aaaaactttt gattttgaaa
    12841 gatttaatgt aattgatgct atttatggaa ataaaccatt tgaaaaagat ggaaaagtta
    12901 ttgtttttaa tggaaatata tataactatc atgaaataaa agaagaatta attaaaaaag
    12961 gacacaattt tactacaaat gatgatattg aagtcttatt ggcatcttat atagaatttg
    13021 gtaagaattg tgtacataag attaaaggaa tgttcaactt tataatctat gatagagaaa
    13081 atgaaagtat atttggagca agagatttat ttgggataaa accactctat tatataaata
    13141 aggaaaatgc aataattttt tcctcagagt ataagttttt acttgaatat atgaaaaatt
    13201 taaatataaa tgaaagaagc ttacaaagtt atttttcatt tcaatatgta cttcctgaag
    13261 atactatgat acaaggaata aggctgatac cagcaggtca ctattttagg gtagaaaatg
    13321 gtatactatc tttaaagaga tataataaac ttgagtttag aagtagtaca aaattttttt
    13381 atactaaaaa tcacttaggc aatagagatg tgaacgagga tgatataaga aatatagtag
    13441 tagattcaat agtaactcat atggaagagg agaaagaaat tggaacattt ctgtctggtg
    13501 gaatagattc atctattata actactgtgg cttcacaaat taatccaaat ataaaatctt
    13561 tttctgcagg ctttagtgtc aaaggatata gtgaattaga agtagcaaag aaaacagctg
    13621 ataagttagg tatagaaaat atccagatta atataacaca agatgaatat ataaaatcat
    13681 taccgaatgt aatttattct ttggatgacc ctatagcaga cccttcagaa gtaggtcttt
    13741 atttcttaat taaagaggca ggtaaacatg ttaaagtagc attgtctgga gaaggagcag
    13801 atgaattatt tggaggatat aatatatata aagagtatag tactatgaaa tcagttgtca
    13861 attcacctac ttatattaaa agtatattag gaagagtttc agaacttatg ccaaacataa
    13921 aaggaagaaa ttatctttat agagctacaa caccattaga aaaaagatat ataggaaatg
    13981 caaaagtttt tgaaaacagc gaagtaaaaa gattcttttt taagtataag gaaaagaata
    14041 tatatgaata cctgttgtct aatttgtata gagatgcaca aaaaaataat tacgattata
    14101 taagtaaaat gcaacatata gatgtgaata catggcttca aggcgatata cttcaaaaaa
    14161 ttagcaaact ttctacagct gagcaggttg aacttagagt accattttta tataaagatg
    14221 tatttgatgt agcaaaaaat ttaagaatgg agcaaaaaat aaataagaat aatacaaaag
    14281 ttttacttag agaggcgttt agagaaatag tcccagaaca tgttgttcaa aggaaaaaat
    14341 taggatttcc aactccaata agagtttggt taaaagattc tctaggggat attgtaaagg
    14401 aaactattta taattcaaat gtagatgaat ttatagataa aaaatatgca ataaaacttt
    14461 tagatacgca tcttaaaggt catagagata attcgagaaa aatatggact atatttacat
    14521 tttgtctatg gcatcaatta tttattgagc ataaaaatgt cgagtattaa tacttttttc
    14581 aatattaatc agagggcaaa agcatgcaat gttttcaccc tcctgattat tatggaatag
    14641 gataagtaga attttgattg attagtttac tatctcaata aaattattgg caaatatttt
    14701 gacaagatta ctttcaacag ctaaatttaa gttttgtcca tcatcatatc cagcggaaat
    14761 caatcctagt aagtttccaa acatatctaa aagagctcct cctgaactac cactagagat
    14821 tggagctgta aattgtatca tttgaaccgt ttcaaaatca cgaaaagctg aaataattcc
    14881 atctgaaact gtattaaaaa gacctagagg gcttccaatt gcaactattt tttgacctct
    14941 cacaggtttt ttactgactt taacaggtat tggcttgcat tttctatcaa cctttatcac
    15001 agctaaatca taatctgaat ggtattttat aatttggtaa gatgtataaa ctttatcatc
    15061 attttcaaat ctaactaaaa atgaataccc atcattgact acatgaagat tcgtaagtat
    15121 ataaccttca ttatttatta ctactccaga accgctctta aacggtttat tttcgttatt
    15181 gcacacaaca atcataacaa cagaagctga taactttgca aggtcttcat aagaaagttc
    15241 tacttcctca tcttgtacaa tttttttaga tgttagattt gtgttattaa gtgtattttt
    15301 agagttaggt ttattcgtag taggagaagt cttattggta ttttcactgt aatttatatt
    15361 tgtaatatgc ctttcaaaag gtatttctaa tatatttgga gtcttcctag aaagttcaaa
    15421 tagagtagaa aattttgaac attcaggata taaatcaaga atatctttat ttggatttat
    15481 atataaaata tcacacccaa gttgtgataa aaaatataag aaaaaaagtt cttgcctttt
    15541 aatatttcca aagtatacta tctttggtga aatagtagtc ttcaccttgt tataaaataa
    15601 tttaggtaga aaatagtcta tccaacacaa aagtttaact gcaaagtttt tgatgataga
    15661 ggatgtacaa tttggcatac ttgtggaaaa taaatctaaa gtatttttaa atgcaatctt
    15721 tttagtccaa tttaaggtgt cattttctat tttacatgga aagtcgatat tatataaatc
    15781 ccaactgtta aacttagaac tattataaat attaaaaatt ttaagcatct tatctgtttg
    15841 ttctttgtca aaatttattt ttagattgtt taatctaata tagggaattg taggattatt
    15901 aaaaaaataa gtatctatat ttttaatttt ttctatgtat ttatcaattg aatcatcaac
    15961 acctatatat cttgtaaagt agacttcttt aatatatgaa gaacgcttat ttttataggt
    16021 agtatcagat ataaattcaa ttagaggttc agtagacttt tttattgaaa tctttaatgg
    16081 tttaatagta caaatcatat tttttctcct ataataagat gatataatct attataaaac
    16141 aacttaaaac aatatagtac aataattttg tagtttcaaa ataaaatatg tacttgttta
    16201 tataaatgat atataattac atattaaaca tagcttttat gcatttaata aagtatgtaa
    16261 aatctatgta aaaagtaaga aatataaaca tttttttaat tatattttaa aaatatcaag
    16321 caagttttat tcacaaatga ataaaaaatg ttataatatg attaaagaaa aagataaata
    16381 attagcaaga tttgtttagc taaaattaac aagggagcga taaattatgg gtataacatt
    16441 atcaaaaggc caaaaggttg atttaactaa aggaaatcca ggtcttaaaa atattttagt
    16501 aggtttagga tgggatacta acaagtatga tggtggattt gactttgatt tagatacagc
    16561 agcattttta acaggagcaa gtggaagtgt tactaatgat ggagattttg tattttacaa
    16621 caatttaaaa catacatctg gagcagttga gcatctagga gataacagaa ctggcgaagg
    16681 tgatggagat gatgaacaaa tagttgttga tttatctaag attcctggag aaataagcaa
    16741 gatttctttt actgttacaa ttcatgatgc aacagaaaga agacaaaatt ttggacaagt
    16801 aagtaattct tatataagaa tagtagataa agacacaaat gaagagttaa taaagtatga
    16861 attaggagaa gattttagta tagaaacagc tatagtagta gctgaaatat ataagcacaa
    16921 tggagaatgg aagtttaatg cactaggttc tggctttgaa ggtggtcttg ctgcattatg
    16981 tggaaacttt ggaataaact tataatttca actataacaa taaactaaag ggggatattt
    17041 tccatggcta tagtattaaa aaaaggacaa aaaatcgatt taactaaggg aaatccaggt
    17101 cttaaaaata taaaacttgg actaggatgg gatacaaatt catttgatag tggctatgat
    17161 tatgatttag atgtaagtgt tttcatggtt ggagaatctc aaagagttga aaaagatgaa
    17221 gattttatat tctacaataa cttaaaacat ccatctggag cagttgaaca cttaggtgat
    17281 aatagaactg gtgaaggtga tggagatgat gaggaaatat tagtgaattt agagacaatg
    17341 ccaaagcata tacaaaagat agctgtaaca gttacaatat atgaggctgc tgaaagaaga
    17401 caaaattttg gacaagtaag caactcatat ataagagtat taaattctga aaatgatgaa
    17461 gaaatattaa gatatgactt aggtgaagaa tttagtatag aaactgcaat tacagtatgt
    17521 gagatatata agtataatgg agaatggaag tttagtgcag ttggagcagg atttgaaggt
    17581 ggcttagaag cactttgtag aaactatggc ttaaatgttt aatttaagca atataatata
    17641 ttaaatataa ataacctata caaataatgt ataggttata ttatataaaa ttttgtggag
    17701 gtttagtatg tcgattaatt tatcaaaagg tgataaaata gatttaaaaa aatctaaccc
    17761 aggactgagt aacatacttg taggtctagg atgggaccct gtgcaacaat ctggcggagg
    17821 tttttttaaa tcgttatttg gtggaggaca agctgatata gattgtgatg catctgtatt
    17881 tatgcttaat caagaaggta aattaagtgg aataaaagat ttaatttatt ttggaaatct
    17941 aaaaagtgcc tgtaaatcag ttttgcatac aggagacaat ctaactggtg agggtacagg
    18001 tgatgatgaa caaatcctag ttaatttgga taaagttcca tcaaatattc ataaattatt
    18061 gtttgtagta aatatataca attgtgtaga tagaaaacaa cactttggaa tgatagaaaa
    18121 tgcctatata agagttgaag accaaggtaa taagaaagag atagcaaaat acaatctatc
    18181 tgataattat tctgaaaaaa ctactcttat agtaggagct atatatagaa aagatggaag
    18241 ttggcaattt aaagcaattg gtgaaggaac aaaagatgcg ggtcttaaag aagtaatgca
    18301 aaatttagac aggattgagt gtgcatatgg aatttaataa tgatatcaaa tattataatg
    18361 tagataaaga agaagttaca aaagacttat ctgttaatcc tgaaaaaggt ttatcagaat
    18421 cagaagtcaa gactagaaga gaaaaatatg gtttgaatga atttactcca aaagaagagg
    18481 gtagtttttg ggatgattta aaggaaagtt taagtgaacc aatgatagtt atcttgatta
    18541 ttgcagcagt agtaagtgca gtaattgggg aaactcatga tgcaataggg attgtaggtg
    18601 ctatagcaat aggtatagca ataggtatga ttacagaagg tcgttctaag aaagcagcag
    18661 aggctctttc taagttgact gaaaacatag aagtaaaagt attgagagat ggggaagtac
    18721 atcaaatttc taagagtgaa ttggtaccag gtgatatagt atatattgaa actggtgaca
    18781 tggtacctgc tgatggtaga cttatagaaa gtataaacct taaaattaga gaagatatgt
    18841 taacaggaga atctgatgac gtatctaaaa agtgtgatgt agtagtttct atggaagaga
    18901 tagaatctaa aggagcagta gtagtacagg aaccaatacc tgcaaaacag ataaatatgg
    18961 tgtttggtgg tacacttgta gcttatggta gaggagctct agttgtaact tcaattggag
    19021 ataatagtga aatgggtaaa attgctcaaa acttatcaga aacagatgag caaactccac
    19081 ttcagataaa acttggaaat ttaggtggtt tgatagccaa agtttctagt gcagtagcag
    19141 gattactatt tatatttatg gttttccaaa tgataagtaa gaatgtatta aatgtagata
    19201 tgtctggaat attaccgttt ttagaatcaa tagacccagt taaaacagca tttacagtat
    19261 gtgttgcgtt gatagttgca gcagtgccag aaggtcttcc aactatgata aatatgacac
    19321 ttgcaataac aatgcaaaaa atggctaaaa taaatgcatt ggttaccaaa aaagaagctt
    19381 gtgaaacgat aggttctgtt tctgtaatat gttcagataa aactggtaca ttaactgaaa
    19441 atagaatgac tgtagaagtt gcatatgttg atggcagata tattgaaagt tcagaagagg
    19501 aaataaatag ttattttgaa gaaaactgta tgataaactc tacagctgat gtagagcaca
    19561 atgatggtga tattaagtat ttaggaagtg ctactgaatg tgcattgctt ttatattaca
    19621 aaaacgtaga ttatagacaa gttagaaaaa attctaatat agtagctcaa aatcctttta
    19681 cttcagattc aaagagaatg acttctgtta taggtcaaga taatcatcat gtactattat
    19741 caaaaggagc accagaagtc ttacttgaat tatgttcaca tattcaaaga ggaaaagaca
    19801 tagttccatt aactgaaaat ataaaacatg agattttaga agaaataaag aaattgcaaa
    19861 taaaatcaat gagaacttta gggtttgcat ataaagaaat ctcacaagct gaagaagaag
    19921 cagcagtaac tgctgaaagt gatgctatga atgtaagtgc aatggaaaac aatttagtat
    19981 ttagtggttt tgttggtata agagacccac ttagaaaaga tgttatagag tctgtaaata
    20041 cagcaaataa agctggtgtt tctgtaaaaa tgcttacagg agataatata aatacggcaa
    20101 gagctatagg tgaagagtta ggcctattaa aaaataatat gagagtggtt gaagcttcat
    20161 atatagatac tttagatgat gaagaattaa aacaagagat acaaagtatt tcaatcgtgg
    20221 caagaagtaa accagatagt aaaatgagaa tagtttcagc tctgcaaaag agcggagatg
    20281 ttgttgcagt aacaggtgat ggtataaatg atgctccagc actttcaaaa gcagatgttg
    20341 gtatagcaat gggtatatct ggtacagagg tatctaaaaa cgcagcagac ataattttaa
    20401 ctgatgatag ttttagtacc attgtaaaag gtattaaatg gggtagagga atttatgata
    20461 acttccaaag attcgttcaa ttccaattaa ctgtaaatgt aattgcattt ttagtggcta
    20521 taatatctca agttatggga caagagatgc catttactac aatacaatta ttatgggtaa
    20581 acatcattat ggatggacca ccagcactag cattaggttt agagccagtt agggaccatg
    20641 tgttaaacag aaaacctgtt gatagaaaag ccaatataat ttctaagtca atggtgtata
    20701 caatagtatt aaatgcattt tatataacag caatactaat gttacaatct acatttaatt
    20761 tcttaggagt tgatgaagcg cataagggaa cagtaatgtt tgcgttattt gcttttagtg
    20821 cactgtttaa tgcatttaac tgtagagagt ttaatgcaga tagtatcttc cctaatttta
    20881 cacataacaa attagcacta caaataatag tgttgacagg aattgcacaa gtaatattta
    20941 ctcagctatt ccaagatttc tttaactctg tagcaatgga ttttatgaca tgggtaaaag
    21001 ttttatgtgt agcagcaagt gtaattgtgg ttaatgaagt tgtaaaatgt ataataagac
    21061 tggtaaaacc aaaaaagact gcttaaaaca aattttttag aagaaaacag actgactcaa
    21121 aatatgaatt ttgggtcagt tctgaataaa catttaaatt gggaggatag ttatgggtga
    21181 ctcaaaggta tttgaaaaaa tattctccag ccaatcagat agaggaaatt acactccttc
    21241 aaagggatat ctaagttatt ttatttcgta tattggttta gaagatgaag ttttgtacaa
    21301 tctcgagata tttaaaacaa aacaaaatat tgatagcaaa aaagatattg ctttatttac
    21361 tgatgttata gcaaatccta gtgattttga tattataaat tattttaaaa gtggacttca
    21421 aaaatataga actagcatgg aagatgtaga tataaatatt ttgggatttg aagagattga
    21481 ttataaaata aagcaagcaa tggatagagt gttaaaagaa gaggaaaaag aatttactaa
    21541 tgatagagtt aaacaaaatt ttatagtgaa aataatggca tggataaaaa tttatatcgg
    21601 tgctttagat ataaataaaa atgaagctcc aaaagtaata ttttatggag atataaaaaa
    21661 acatgaagtt tatttactct taatattata cttggcaggt tttgatgttt tatatcttaa
    21721 tccaaattca aagagtaata tagatatttt aaaatcagaa agatacaata tagagtttga
    21781 agaagccaac ataatagaag aaaaaatttc ttttgaagaa agggttattt taggggaaaa
    21841 aatagataaa agttctgtaa aaaaagcttt tactgttggg gcagaagcat caaagcgtat
    21901 aagtgaggaa ttgttaaatg atgcaggatt tataaaacct tggcaactcc aagatagaaa
    21961 aataaaaaat ctacttctaa gttcaactgt tgatgaaata tcaatatact ggaatcaacc
    22021 acttaaatta agaccaggat ttaagtttaa tgatgctatt gtagaagctc caaattttct
    22081 ttctaagatt aatggaatat ataacgataa gaatgaatat attaagtttt tagatttatt
    22141 aagagattcg gaaagttcaa catttattga atttaatgga gatgtagata gattttcaaa
    22201 agcttttaca agagaagctt ttagtttatc ctttttattg gattcaaaag gtgcaataga
    22261 taaaaactct gttttaaata ataaagatta ttccatatca acattagcat tgaatcaaca
    22321 aattatgatt ttagagaaag tggaagagtt gttagaagga agtatgtttt tgaatggttt
    22381 aagtggagaa gacaagataa aaggtctatt tacagtactt cacatggata agaaatttgt
    22441 tcatatgatg aacaatttcg attactcact tataaaccca aaattgatta tatacatgta
    22501 caagagtatt gtatttgata aagaaatagt ttttcttatg cttttactta gtaagattgg
    22561 atttgatatt atcatattat gtccaggagg agaaaacaat atagagaatg ttataaataa
    22621 ccaattgatt gatgtacaca gattggataa aatggtatat gaccttaaat taaattcgct
    22681 tgaaaatgat atacctttac ttaaaaaaat ttttggaaag aggagacgat tttagatggg
    22741 gataaatata aatagttttt caaaagataa gaactctgta gaggttactg agaatttgcc
    22801 tacagcagtt caagaagaaa acttcgatat aatggaatat acaaataata aaaaaaatga
    22861 actgagaaag tctaaggaag ttgaggcatt gacatctctt atagaagtag aaaatccaga
    22921 tacg
//
LOCUS       DAMJWS010000241        17103 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.guided.092, whole genome shotgun sequence.
ACCESSION   DAMJWS010000241 DAMJWS010000000
VERSION     DAMJWS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.guided.092"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(159..683)
                     /locus_tag="PC399_003848"
     CDS             complement(159..683)
                     /locus_tag="PC399_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2594593.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(748..1500)
                     /locus_tag="PC399_003849"
     CDS             complement(748..1500)
                     /locus_tag="PC399_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2594594.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1531..2829)
                     /locus_tag="PC399_003850"
     CDS             complement(1531..2829)
                     /locus_tag="PC399_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2594595.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2846..5110)
                     /locus_tag="PC399_003851"
     CDS             complement(2846..5110)
                     /locus_tag="PC399_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2594596.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5365..6183)
                     /locus_tag="PC399_003852"
     CDS             complement(5365..6183)
                     /locus_tag="PC399_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2594597.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6176..6874)
                     /gene="cbiQ"
                     /locus_tag="PC399_003853"
     CDS             complement(6176..6874)
                     /gene="cbiQ"
                     /locus_tag="PC399_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2594598.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6877..7155)
                     /locus_tag="PC399_003854"
     CDS             complement(6877..7155)
                     /locus_tag="PC399_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2594599.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7145..7897)
                     /locus_tag="PC399_003855"
     CDS             complement(7145..7897)
                     /locus_tag="PC399_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2594600.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8079..8271)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8403..10175)
                     /locus_tag="PC399_003856"
     CDS             complement(8403..10175)
                     /locus_tag="PC399_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2594601.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10189..11118)
                     /locus_tag="PC399_003857"
     CDS             complement(10189..11118)
                     /locus_tag="PC399_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2594602.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11347..11535
                     /locus_tag="PC399_003858"
     CDS             11347..11535
                     /locus_tag="PC399_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2594603.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11528..12229
                     /locus_tag="PC399_003859"
     CDS             11528..12229
                     /locus_tag="PC399_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2594604.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12231..13154
                     /locus_tag="PC399_003860"
     CDS             12231..13154
                     /locus_tag="PC399_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2594605.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13311..14234
                     /locus_tag="PC399_003861"
     CDS             13311..14234
                     /locus_tag="PC399_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2594606.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14227..14964
                     /locus_tag="PC399_003862"
     CDS             14227..14964
                     /locus_tag="PC399_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594607.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14975..15748
                     /locus_tag="PC399_003863"
     CDS             14975..15748
                     /locus_tag="PC399_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594608.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(16289..16897)
                     /locus_tag="PC399_003864"
     CDS             complement(16289..16897)
                     /locus_tag="PC399_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2594609.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5518 a         2967 c         2155 g         6463 t
ORIGIN      
        1 gttaaaatca tacaatatat agatttccac aatacaaata aattaaaaag tgaaatgtat
       61 ttgtctaaaa acataataac atttcacttt atcctcattt ttaagtaata tatattttta
      121 atttttattt ataactaaat ctagttttta tataaatttt agtttttttc tacatctgag
      181 taatctattc caaaagtttc tacagtaaca gatttcataa tttgtggagt ttgtggctta
      241 tcagctgaat ctgttctaac tgatgctatt tcatcaactg tatccatacc ttctatcact
      301 ctaccaaaac ctgcatactg accatctaag tgtggtgagt ttttatgcat tatgaaaaat
      361 tgagaaccag ctgaattagg tgccatagtt ctagccattg atatgactcc tctttcatgt
      421 tttaaattgt tagtaaatcc attaccacta aattcacctt ttattgagtg acctggacca
      481 cccattcctg taccatttgg gcatcctcct tgtatcataa atcctggtat tactctatga
      541 aatattattc cattgtaata tccttcattt actaatgata caaagttttt aactgtattt
      601 ggagctatat ttggatataa ctcaattttg atttcttttc cattttccat ttctatagtt
      661 actataggat ttttattttc cataatttat taccttcctt ttcattagtt aaattacata
      721 attacattta ttatattata ctctaaatta aagtctatca agtatatttt ttcctatttc
      781 atatccttct tgataaatag ttttacaaaa ctcatcatca aagttaagcg tgtctttagg
      841 agcaaattga gtattaggtc taactactat tattttatct tcatcatata cttttttaat
      901 atcttctggc aactcataat tatattttgt ggatattaaa ataattttat ccacattatc
      961 cccaattaat ggttcaatta atgcactcct aattaaacct ccatctaatt tgaaaccatc
     1021 ataaattcct tcttgcacat ctttcacaaa ttgttcttta aatccaagag ttgcgtttgg
     1081 atttgatggt aacaacccac tgtatgaaat gctgataatg gcttcattac gaggcatttt
     1141 tgatacatca acaatctttt ctgatactac tttattatca acttcaacat aattaacata
     1201 aaagttcatt tcttctatat tactatcatc tagttccctt aggttgtcta tagcaggaga
     1261 attatccaca gtattattta ctatttttac atttccattc tggatatctt ttatatttgt
     1321 ccacattttc tccaagttat caacattccc tgtgtacaaa taatatccat ttattgctcc
     1381 tattgatgtc cctgaaatcg aataaaattt atttattcct ccatcataca aggctttaac
     1441 tactcctgct tgataagccc cttttgctcc tccaccttct aaacataatc ctaatttcat
     1501 agtctcatct ccaaattgtt tatcttatat ttatatttca aaaaacttac taaatgtttt
     1561 atatatacta tagtggtcct caacatttcc taattctctt atagaatgca ttgctaaaat
     1621 tggacttccc acatccacag atggtatatc tatatgagta gaagatattg gacctatagt
     1681 acttcctcct ctttcatctg acctattaac aaattcttga tactcaacat tagcttctct
     1741 acatatattt ttataaatag ctatagaata tccatcacta gtatacgcct gactagcatt
     1801 tatctttata accggacctt ttcccattat tggtttatta gttgggtcat gcttttctgg
     1861 aatgtttgga tgaatagcat gagctaaatc tgatgatatc ataaaagatg aataaataga
     1921 ctcaaagaat ccctctctat ccttacccat tgatatacaa attctctcca atatatttaa
     1981 caacatgttt gaatctgcac cttgtttagt agagctacca acttcttcat tgtcaaatac
     2041 agccattaca tttacaccat gttgaccttt agtattaaca agagcattca aactagcatg
     2101 taccattgca agattatctt gtctcccaat agatataaat tcatcattag cccctattaa
     2161 acttcctttt tcaaattcat ataaaaataa atcaaaatct ataatatcgt ccatatttac
     2221 atctaagtta gatgctattt gctttataag aaaattatct ttttctaatg tatcatttaa
     2281 catcccaact aaaggtaaca tatctttttg cttattatat gaatgaccat catttatact
     2341 tctatttaaa tgtattgcta gatttggtat tatacaaatt ggctttttaa agtctataag
     2401 cctttcattt ggttttaata tactatcacc ttttaaaact actctaccag ctatcccaag
     2461 aggtctatcc aaccatgtac ttaatattgc tcctccatag cattctgtat ttaacttcaa
     2521 ataagatttt tcagcttcta tttctgcatt tggtttaata ctaaatgtag gagaatctga
     2581 atgagagcct attattctaa atccatctct ttctatatct tctgaattta ctacaaaagc
     2641 tactatagct gataaattct tagtaacata atactttcca ccaactctta gattccatct
     2701 ttgattcaaa actaattctt caaatccatt tttatctaag atttccttag tactcttgac
     2761 tgcatgaaaa gacgacggac tatcatatat gaagtctatt aagtttttag caaagtttat
     2821 actatccatt cctcatttac ctccattata aaatatttag tgttgtatat tctttattta
     2881 tttttttaat tccctgttgg tcaaaagcta tagtaacatc tgtaccaatt atagaaacca
     2941 ctgtcccaac cccaaattta ggatgatgta ccttagaacc aagttttata tcatctggat
     3001 tagtttcttt attagaatct tttattgtag cattaacttt atctgcaact ttagatttgc
     3061 ttatagtact catatacttt ttagtatatt tatctaacat attataactt gctttagaat
     3121 agcttagttc tttttttact ttgtacaatc tttctataca ttcttctggc agttcttcca
     3181 taaatcttga agctattgca acatttgttt taccatataa tgttctcttc tctgtcaatg
     3241 acatatatag ctcttcttta gctctagtta taccaacata acaaagtctt ctctcttctt
     3301 ctatttgtga atcactcata gattttatag ctcttgatat aggaaataac ccttcttcca
     3361 tacctgttaa aaacacaaca gggaactcta aacctttaga tgtatgaatt gtcataagcg
     3421 aaaccctatc attgtcttct tcatcatttg attcagatgt aagtgctata cttgttagga
     3481 atgtctctaa gtctttattt tcataagtat catcattact actttcaaac tccaaagcta
     3541 tagaaataaa ttctctcaaa ttatcaattc tgtcttgagc ttcttcacct ttgcctgtta
     3601 aatctttttc atttttattt cttatctcta caagctcgtc catatatcct gtagtgtcca
     3661 agactttttc tataagtttg ctaacaggat aaacttcttt tatagttcta agagtcccta
     3721 taatatcaac aaatccactt atacttgccc ttgccttagt agatatatct gaatttgttt
     3781 ctatatcaat taatactgag tatatacttt cctgttttaa gttagctcta tcttcaattt
     3841 tttctatggt tctaagccct atacctcttc taggaacatt tataatcctt tttatagata
     3901 tatcatcttg tggattttga ataactctta aatacgccaa caaatccttt atttcttttc
     3961 tctcatagaa ttttgtacca ccatatatat tgtatggaat ttgacttcta tttaaagcgt
     4021 cctctacagg tcttgcctgt gcatttgctc tataaagtac tgcaaaatcc ttataaggtc
     4081 tattttcttt tctagctatt tgtgcaattg aatcagcaat gaaatcagcc tcttctattt
     4141 cactaccagt taattgtatc tttataagtt ctccttcttt tttttcactc caaagctttt
     4201 ttctttttct ttctatatta ttagatataa ctttattagc tgcatctaat atcacttgtg
     4261 tagacctata attttgttcc aactttacaa catgtacatt gtcataatct ttctcaaatt
     4321 caagtatgtt tcttatatct gcacctctcc atccataaat actttggtca tcatctccaa
     4381 caacacaaat attttgatgt tctcttgcta ataattttat caattcatat tgagctttac
     4441 ttgtatcttg gtactcatct accattatgt atctaaatct acttctgtaa tatgctaaca
     4501 cttcatcatt agccttgaaa agttctacag ttttaagtat taaatcatca aagtcaagtg
     4561 cactatttct ttttaaacgg tcttgatata gcgcataaat atctgcaatc ttaaccattc
     4621 tattatctgc catattgata tctttaaact gctttggagt ataaagttta tcttttgctc
     4681 ctgaaattgc acttataact gcttttggtt caaacacttt atcacttaaa tttaattctt
     4741 ttagacagtc tttaaccaat gtaacttggt ctgctgaatc ataaattaca aaacttctat
     4801 tgtaaccaat cttatttata tcttttctta gtatacgaac acagcaagaa tggaatgtac
     4861 ttatccacat atccttagtt tcaggaccta tattttgttc aactctctct ctcatttcat
     4921 ttgcagcttt gtttgtaaat gttatagcta atatatttgg tgcttgtaca cctttatctt
     4981 ctataagata tgctattctt gttgttaata ccttagtttt acctgaacca gcaccagcca
     5041 gtataagtac tggaccttca gtcttttcta ctgcttctct ttgagcagga tttaatgtat
     5101 ctaaattcat atttctcctc ctgttaacag ccataaatat attttaatct aaaatatgaa
     5161 ttacttcaaa ttataattat gtattatatt aatagattat ttccaatata ttacaattaa
     5221 ataacatttt tccatataat ttatttcatt gtttaataaa aaaaataacc ttatgatttg
     5281 gggaatcata aggcaccttt aaggggtcta ggttaagggt tattatatat aaatctattt
     5341 ttttaaaata ggtttagggc ttaattattt atttgtatta agttctcttt ccttccaata
     5401 cttatacaaa tcttcttctt tcttaaataa gggtaaattc atatttctat gtacttttac
     5461 taaccatggg gattctaggc cagcttgtat aatattattt tcatttataa acacattttc
     5521 tgcttttcct tcatcaatca aaatcccctt atccaataca tatatgtaat cacatatttc
     5581 atacattaaa ttcatatcat gactggatat tactattttt atattatttt tatttaatcc
     5641 ctttattata tctacaatag acctagtact tactgggtct aaaccagctg taggttcatc
     5701 aagtaataca atttcatttt ccattgctat aactgatgct attgccactc tttttttctg
     5761 tccatagcta agaaaatgta ctggcctgtc tataaaatct attccattaa cagctactag
     5821 agctttgttt attctctctt taattgtttt ttcatccata ccaatgtttc tcattgcaaa
     5881 ggctatatca tcatatactt ttgaataaaa aatttgtttt tctgggtctt ggaacacaat
     5941 acctacattt tttctaaggt tgtataaact tctcttatca tattttaact tttcttcttt
     6001 aaaaagaatt tctccagatg taggctttaa tatacccatt aaattcataa ataatgttga
     6061 ttttcctgaa ccatttgaac ctattatacc tataacattt ccctttgaaa aatccatatt
     6121 aatgtttaat agtgcattgg tatttttttc atattgatac gttaaattat ttattttaaa
     6181 catcattatc ccctactatg tgaaatttac catcatacaa ttttatgtct aatgatatac
     6241 acatatcatc ataccttgtc ataagtcttt tgtacagcat acttccaaga atacctaagg
     6301 atttatatga agtttttaaa tttatatatc caaatcttag ctcttgtgac ttgtatattt
     6361 ctgaaaattc ttctaaaaat atgaatataa acctatatat taacattgat aactcgacaa
     6421 aagtgtcagg taaatgaagt tttttaaaaa agaatattaa ttgattaaaa ggtgttgtaa
     6481 gtatacaaaa atacacacaa gtcaaacaag acatcgctct aaataacaca tgaatagaca
     6541 tgtcaataga cgcctttgat attcctacat taaaactaaa aaattcaaaa ctatataata
     6601 aatctgcttt attaaaagat atatttacaa gagttattcc tatactaaga aataaaaagt
     6661 acattggtat tctaagtaat tttaaataat tttttaaatc tattccagcc acacaaacaa
     6721 ccaaaatact cattaaaatc ataattccac ttaaaataaa tatattttgt attaccatag
     6781 aagcaatcag aaaaattact cctatggcta cttttttgtt tggatttact ttactcaatc
     6841 tatttgtata tgcatattta tcaattataa gcatgattaa cttctctttc ctttttgaag
     6901 acctaatatg tatcctatta caccagcccc taaagctgct tgtgcagaga ataataaact
     6961 ttctatttct ccacttggtg gctcatatgg actgctaaac catggtttgt aatttggatt
     7021 tatcttagta atttcacttt cagcttgacc atctgctcct ccatattcag cacctgagtt
     7081 gacaagtaat gggaatataa taagagctac tactaataaa agtaataata tgttagtttt
     7141 tgttttagca ctcattagct ttaactcctt tatcattatt ttctagtatg cttttttctc
     7201 cgttttctgt aattaaatta tatacaatta cagtaagtat accttctgct attgctattg
     7261 gtatttgagt cataaagaaa attcctaaaa atttaattgc tgaagccatt accccacctg
     7321 atgggtctgg gaaagccagt gctaattgta ttgaagttat tgtatatgtg gctaagtctc
     7381 ctattgctgc tgcaagaaat actggaatag cattatttcc atcttttttc tttaaagcct
     7441 taaatattag gaatgatacg attggcccta ttatcgccat tgaaaaagca ttagctccta
     7501 aagtagttat tcctccatgt gctaataaca atgcttggaa tattaaaact atagttccaa
     7561 gaacaaacat tacgcttggt ccaaacatta ttgctccaag acctacacca gtaggatgtg
     7621 aacaacttcc tgttactgaa ggtattttta gtgctgataa tacaaacaca aatgctccac
     7681 ataaagctaa taatactttc tttttagggt cttgctttac tattttacct attgatttta
     7741 atccgaataa gaaaaatggt ataaatataa ctccccatgc tatagaccat tttactggta
     7801 agtaaccttc cattatatgc attgcatttg atgtaactgg agttaaaatg attaacataa
     7861 gtgcagctat aacccctaat ttaatattct gtttcatctt agtatccctc tctccctttc
     7921 taataattaa aacataataa tacatgtctg atttatttaa taaaataaaa aagcctaact
     7981 cgatggttag gcttctaatt ttccacaatc taaatacaaa cacataaaac aaacatatct
     8041 gtataattat tataagaaac tttctccatc cccgaagaaa ttactttaat attttaggca
     8101 ggtctcctga cttagcttca tcctactcct cttccttccc atgagatata aaaaatatca
     8161 tcatagtggc taacaaaagt ttcgtctgca tcacagtagc gggggctgta aaggaattta
     8221 acctttttcc ctattaatct tgcttaaaga acctataata ttatatattt aatttttaaa
     8281 ttcttcttaa attattttct atataaatca ttgtatacct tttctgtgaa aatttctaca
     8341 ttttttataa attttattca tttttatatt tttatgtata taaattcttt tttgtaagtt
     8401 tattaatttc taattatctt ctttaaatac attgaaacta catcagaaaa tctagttata
     8461 tctcttgaat atataaaatc atttccatat attaatcttt cagcttcaag ttctgattct
     8521 tttcctgtaa atactcctaa tactaatata ttttgctttc tagcttttct cacttcattg
     8581 gctgtatctc tcaaaccaac tataccccta taagcctttt ctcctcttaa tgttctctca
     8641 ctactttttc ctatctttac atcatttggt cttccatcac tgagaactat caggatttta
     8701 ttttcttcat ctctatttat tagaccacta catatagatt ttattgccaa accatctcta
     8761 ttattaccag cacaaaaata ttcaaatata ttttcactat tttttatact atcatcataa
     8821 tctttaaatc tttttaatat tgtatagtct aaaaaactag aaaatcccat cactctattt
     8881 ggtatgccaa cttctgttag agcatttgca attatgtaag cttgaattgc cacatttccc
     8941 tgatttctac tttgagagcc acttgcatcc aataatatat caataacgta tttccctttt
     9001 tcattttcta tatctttata aaatatttta ttattattac ttctaccaac tctccaagct
     9061 ctattggcta atatagtacc acaatcagaa tatactctac ttttagcatt ttcttctatt
     9121 aaaattcttg atagactttc ttttaatctc tttatttgct gtctatacat tctagcatgt
     9181 tctctaaatt ttcctaaatt attttctttt tgtcttgtta catattttag ttgaaataca
     9241 ttattacact cacttctaag aactccatct gtaaagtgta ttctacaacc ttcatgaaca
     9301 tttctacaat tttgtgtctc aattttttta atttcacctt tactcaaata agtttttcca
     9361 taataatgtt ctatttttga atatatctta tttgcagtgt cttcatcaac atatataact
     9421 cggtctgctg aattgctaca tttagaatca tcaaaatccc ctacaccttc tacaagcatg
     9481 gatgaagata ttgaatctat atcactttca ataatatttt cttcttcatc actataaagt
     9541 tcttcataca tgaaatcagc aaatgtatca aaatttactt ctacactttc tacattattt
     9601 tcatcaataa gactattttc ttctccattt aatatgtatt caaaataagt taaatatata
     9661 acatctaatg cttttaaaat ctccataata tcttgattgg tatctatatt tcttatatca
     9721 ttaactattc ttctagtcat actatctaat gctgggtgct tgtccatact ataaagtaca
     9781 aatgtgtatt taattttatc cataatatta tcattgtgta ttttggtaaa acgtgaaaca
     9841 atatctttat aagcatttct tcttatgtct tctatccctg gcctatcttt aatgaccttt
     9901 tcttcaacta ttgtatttaa tataacttga attagaccca gtataatatc tttgttgtat
     9961 ccttttctta ttctatttgt aagatatttt ttaactatat cccaatctac atactttctt
    10021 ctagcacctg caattattgc aaaatataga cctatttctt ttgaagagta atctttttct
    10081 gatatatcta tactttcatt gtaatcacca gctattgtcc atgataagtt attagctcta
    10141 ttttcaaatt cataatcatc atatatccat ttcataatct cacctatatt aaatatgact
    10201 tattggaaac acatctatac tctcccattt attaggtatt ctagttttaa ttacatctcc
    10261 aaccatttct ttctcatata catcaaaagt ctttcctgta agtcccatat taattgcaag
    10321 agttggtttt aatcctcttc ttatagtctt aagagatgcc ataagaccac gtaaatcaat
    10381 agctttacta gatatctctc cattttgaga cttcaattgt aaatctaaaa atattcctgc
    10441 aaagtgtatt agtttttctt catcagcatc tgaaaattca ttttttaaaa tcatcattaa
    10501 cttatcttct tctataggag gaatatctat taccataaat ctagatacca gtgcttcatt
    10561 aagttctttt gttcctgcat attcataatt catagtccca ataaatcttg ttgctggatg
    10621 tagattgact ctctcatacc caggtacatc aattattctt ctatagtcta aagctgaatg
    10681 taccacaact atagcatcat tttttgccat attaatttca tcaaacaccc caaaacctcc
    10741 attaatagca cactcataaa ctgagcctct tctaagttta acttcattgt ctataaatgt
    10801 atctgtacct ataagtgttg aactgtctgt atttatatga aatgatgtat tccactgagg
    10861 tctattaaat atttccccta gattatcagc aagtaagttt tttcccgttg cctttggtcc
    10921 cgataataat atattttctt cttctaaaat agctgctata cacatagaca agatatcttt
    10981 accataaaaa taagttttag actttgttac tctgtattct aaatcttttt ctaatccata
    11041 ataatttcta aaatacagta catcttcaat aagtttttca tctacccctt gcacccttag
    11101 attatctatt atatccatat ttttacccct tttatataaa tagtatttgt ttcatttctt
    11161 atcaccattt tagcataaaa ggatataagc taaaattgat tttttaatct cccccatttt
    11221 aagtaaaacc atgcaccatt ttatcactac aaaattaaga gtactactta ctgatatttc
    11281 ttgattttat tcgtctatac gtatataata tatactgata aattatgagg aaaggatgat
    11341 tgaaatatgg aatatatgtc ttgcccagaa gtagcaaaaa aatggggtat ttctgaaaga
    11401 agagtacaaa aactatgtga agaaagtcgc ataccaggag tttcaaaact cgggtatatg
    11461 tggctgatac caaagaacgc agaaaagcct gttgatggta gaacaaaaag aagaaaggaa
    11521 gtacaatatg aatagcgttt tgattataga tgatgacaaa gaactttgtg ctcttatgaa
    11581 aaaatgtgtt gagcaagaaa atttatctgc tattactgca catggtggtg cagaaggtct
    11641 acatttagtc gataaaaata gacacacact ctctcttgta attttggaca taatgatgcc
    11701 agatatagac ggttttcaag ttctgaaaaa aattagagaa acaagtaata ttccagtaat
    11761 tatgcttact gctaaaaatg acgaggatga taaagtttcc ggactacgtt taggtgctga
    11821 cgattaccta acaaaaccgt tcagcataaa cgaacttatg gctcgtgtca attctcttat
    11881 tcgtcggtat actatgctaa accctgcttt tataacagac acagatttca taataataaa
    11941 tggcatgatt attgacaaga caaaccgaat tgtttctgtc aataatattc ctgttcaact
    12001 tacaagcaaa gaatttgatt tacttgcttt tctggcttct aataagggac gggtgtttac
    12061 caaaaaacag atttatacac aagtatggga agaagaatat gcttttgacg atagtaacat
    12121 catgtctttc atcagtaaat tgagaaaaaa gattgaacca gaccccaacc atccatttta
    12181 tattttgaca gttcgtggtg tcggttatcg ttttaataag gaggcttaac atgaatacaa
    12241 gtgtctatct gcttttgata ttggctctta gcctgattat tatagcgtac cttgtttctg
    12301 tcctgctacg tgtccgaata caacttacac ttataaaaga tgctttagag gatataaaaa
    12361 atggaaacct taatcgacgt attttaacag atgaaaacga cattacaaaa caaatttgct
    12421 atgatattaa cgaaatagca atcaatagcc aatttcaact tattcagcaa aaacaatcag
    12481 aacaagccta caaacagcta atgacaagtc tctcccatga tgtaaaaact ccacttgctt
    12541 ctttggtcgg ttatttggaa gcagtagaaa gtaaaattgt tgtaggagag gaaaaagacg
    12601 agtatatcca tgttgcgtct aataaggctc attacttaaa gaattttgta gaaaatctat
    12661 ttgaatgggt aaaattggat tctaaggaac aagtttttca ttttgatatt tttgacttga
    12721 atgaattatc ccgtaatatt atatctgatt ggatacctgt tttagaaagc agtcattttg
    12781 aatatgaatt tgatatacca gaaatagagt acttcttacg tatagacgca aatgcatata
    12841 cccgtatcat taataaccta ttacaaaata ttattaccca tagtagtgga aacaaaatga
    12901 cattgagaat ttttgaaaac aaagagcaag ctcaaattat aataacagat aatgggaaag
    12961 gtatatcttc agacaatctc ccacatattt ttgaaaggtt gtaccaatgc gaccattcac
    13021 gagcttcaaa gggaaatggt ttaggcttgg cgattgcaaa ggaacttatt aacgctcata
    13081 agggaaatat taccgccaac agcactcccg gtatgggaac agaatttaca atattgcttc
    13141 ctaaagccct gtaaaagtac gaggctttct tactctaaaa caagataaaa gcaagattat
    13201 ggcaaggttt tggcaaggtt catgttttat aattctaatt gtagttgatt ttgacgttgt
    13261 atcaactatc ttttgtagaa acaaatttat taaaagtgag gaaattatat atgaatgatt
    13321 ttgtgattga aacaaagcaa cttacaaaaa tttatgggaa tcaaacagta gttgacaaag
    13381 tcaatctcca tgtaaaaaaa ggccgaatat atggtctgtt aggacgcaac ggagctggga
    13441 aaaccacaat tatgaaaatg attttaggat tgacttcaat cacgtatggc gaggtagatg
    13501 tatttggaga gaatattaag ggacgtgaaa aacaagtata tccccgtatc ggtgcaatta
    13561 tcgaaacgcc aggattttat cctaatctga caggaacgga gaacttggag atttttgcaa
    13621 aactgcgtgg tacagccgcc cctaattctg taaaaagagc attggaaatt gtaggcttac
    13681 cttataaaaa taaaaagctg tttagtaaat attcacttgg catgaaacag cgacttggta
    13741 ttgccaacgc tatcctgcat gacccagaac tgttagtttt agatgagcca accaatggtc
    13801 ttgaccctat tggtattgcg gagatgagaa agtttattag ggacttgagc gttgagcatg
    13861 gaaagacaat tcttatttcc agtcatattc tttcagaaat ctcattgttg gctgatgata
    13921 ttggaattat cgaccacggt gttcttttgg aagaaagtag tatgagtgaa ctagaaaaga
    13981 aaaacagtaa atatatcctg ctacaagttt cagatattcc gaagacatca cttattttag
    14041 agcgtcaatt taacacgaag gattattccg ttcaagatga ctacacgtta cacctttata
    14101 acactaatct ggatatgggt gaaatcaata aagcacttgt aatgcagaat gtaactgtta
    14161 ttagttctca actttgtaat gatactttag aggactactt taaaaaaatc acaggaggag
    14221 agggcattgc ttaaacttat tcaaacagaa tttcttaaac tgcgtcgtag gaaacttatt
    14281 tggctgatgt tgttgtctgc tctcattatg ccgtttttcg cactactata cttcaattat
    14341 tttggtaaaa caggtgttga gccaataaag ttttataaat ggtctgcatt tggttataca
    14401 ctttggatta ttttgcctgt tgttttgggt atattttgca ccatgcttat acacgaagaa
    14461 aaccagtatg atatgctcaa acagctttgg attgtaccta ttagtaaaat gggatacttt
    14521 tttagtaaat ttttcgttgt tttgatttac tctatttgtt ttatgctgat tacagccgtt
    14581 gcttctgtgt tatttagtgt actttctgga tatgtcgtat ttagctgggc aagcatttta
    14641 tatttgttga aaaaatgttt agaaattggt gtaatcactg cttttgtaat gttgccgatt
    14701 ttggcaatcg ctacatctca aaaaggatac attctcccaa tttgtatcac attggtatat
    14761 gccttttccg gttttgttat aatgacaata aatatgtatt tacacccatt atccagtatg
    14821 ggagttatta tcatgagaaa taaagacatt ccaggtctta tattttcaca ggaaattaat
    14881 gtcccattag catttctttg tatttgtatt tggagtattg tatcagtatt gtttgcaaat
    14941 attgcactag gaagaagaaa gtgaggtgta gataatgtgg aaattacttt gggctgaatg
    15001 tcaaaaaatt cgtcgttcaa aaattgtttg gattacagtt tttgcatcca ttatgatagc
    15061 agtcatagtt tttgcagggg ggcaggatgt atataatgac ccagatttac attatggctt
    15121 aaagagcgtt tatgacggtt cacggtatat agacaatgca ggttggtata tggacgcagt
    15181 acaaccttgg tcaacttttt ttgttcttcc tgctgtcatt gccctattag gaagctatat
    15241 tatttgtcgt gaagaagagg aggacactat taaatccctg cgtcttattc ccataaatga
    15301 aacaaattta accatagcaa aaatgattat tacttttata ttcagtatac ttctttattt
    15361 gttactcttt actataacat ttttgactga atccgtttta catttttccg atttatcgac
    15421 aaaattggtt ttaagttgca tgaaagagta ttttcttgat ggcataggtg tattctttgc
    15481 aatttctccc attattgcct tggtatcgcg catgaaaaaa ggatattggc ttgcattagt
    15541 attcacagaa gtttattcta ttgctggact atttgcgggt atgtcaaatg tcttacaaac
    15601 cttttttcct attgcggcaa tatttaatat ttctggatac tatataacta ctggagaaaa
    15661 aattatgggg agtatcatga gtttattact ttgtgcctgc ctttctgcat ttatacttaa
    15721 agggctaaaa cacaatgaaa aaaattgaaa taggtatatc aaaatgaaaa agataaaaaa
    15781 actgattatt ctaattgtat tcttgtttct ttttggcatg ttacactgtt atactgaatg
    15841 gaaattcaac aataacccac ctaaaggggt aatagaaaga ataaatgagt ggactgataa
    15901 gattggactg gattagtaac ttatgttaat tatagtgaat gtaatattag caagttattg
    15961 tttcgtattt taatactata actttaaata tttggtttta atcttccaaa cgaatataaa
    16021 aaaactgtca cagaggcaac atatttttga atatttataa agtggtagtt ttgattttca
    16081 aagctaccgc cttttcttct atataagcaa acttatcctc aacaaagtgt aaaacatggt
    16141 gcattttaag aaaaaagtac cacagacaca agcaataaaa gaaacgccga gaacctacaa
    16201 aaacgtagtg cttatgcaga tttacgacgc cttctatttt ctcaaccgtc ttaatgacct
    16261 tgtacaaaag aaatttttct aatatttttt ataatcttat cttgtgatgt tttttatgta
    16321 taaatgactt aataacaaat ccaataaaaa ttatggatat tggtataatt agaacgagga
    16381 atttataatc attataatgt gacattattc ttacccaact ctcagaaaaa atatatccta
    16441 tactgattaa tgaagtattc catatggtta ttcccacgat agatgatatt acaaaagtta
    16501 taatattttg acctgctata cctgctataa aagatatgta tgttctacat aaagggatta
    16561 atctacatac acaaacagaa atagaagagt atttattaaa ataatcttga gatgcaaata
    16621 ttcctttctt tgcctttgga catatctcta ttattttatt tatgatagat ttacctcccc
    16681 aatttcccac tgtataacaa aatatcgaac caagtatacc tgcaattata cttaaaatta
    16741 aaattgtcag aaatccaaaa tgatttcttg cagcaattgc tccacataaa ggtaaaacaa
    16801 cttcgctagg taatggaaaa caagcatact caatcataat taatacaaat acagagatta
    16861 atccatattg taaaataaaa ctattgatta cagacactta cttatctatc tcctttctta
    16921 tttaaaggtt ttaagggtac atgttctaag gattctcacc ctatatttaa agaatcaatc
    16981 tctttaaact attatatgta tttacacaaa gcatttataa catactttaa aaatagtacc
    17041 ttaaaataag aaacttaata aataaaaaaa gattacgtgg ctacgtccta ctctcccagg
    17101 cag
//
LOCUS       DAMJWS010000242        16124 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.guided.094, whole genome shotgun sequence.
ACCESSION   DAMJWS010000242 DAMJWS010000000
VERSION     DAMJWS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.guided.094"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            complement(120..812)
                     /locus_tag="PC399_003865"
     CDS             complement(120..812)
                     /locus_tag="PC399_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2594610.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(838..2025)
                     /locus_tag="PC399_003866"
     CDS             complement(838..2025)
                     /locus_tag="PC399_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2594611.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            2361..4238
                     /locus_tag="PC399_003867"
     CDS             2361..4238
                     /locus_tag="PC399_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2594612.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKVGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            4548..5954
                     /locus_tag="PC399_003868"
     CDS             4548..5954
                     /locus_tag="PC399_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2594613.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDIYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            5982..6830
                     /locus_tag="PC399_003869"
     CDS             5982..6830
                     /locus_tag="PC399_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2594614.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNEKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            7332..8024
                     /locus_tag="PC399_003870"
     CDS             7332..8024
                     /locus_tag="PC399_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2594615.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            8021..8956
                     /locus_tag="PC399_003871"
     CDS             8021..8956
                     /locus_tag="PC399_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2594616.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            9104..10027
                     /locus_tag="PC399_003872"
     CDS             9104..10027
                     /locus_tag="PC399_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2594617.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            10011..10781
                     /locus_tag="PC399_003873"
     CDS             10011..10781
                     /locus_tag="PC399_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594618.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TVLRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            10791..11573
                     /locus_tag="PC399_003874"
     CDS             10791..11573
                     /locus_tag="PC399_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594619.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            11950..12486
                     /locus_tag="PC399_003875"
     CDS             11950..12486
                     /locus_tag="PC399_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2594620.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            12518..12907
                     /locus_tag="PC399_003876"
     CDS             12518..12907
                     /locus_tag="PC399_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2594621.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            12944..13330
                     /locus_tag="PC399_003877"
     CDS             12944..13330
                     /locus_tag="PC399_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2594622.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            13457..14899
                     /locus_tag="PC399_003878"
     CDS             13457..14899
                     /locus_tag="PC399_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2594623.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            15196..16011
                     /locus_tag="PC399_003879"
     CDS             15196..16011
                     /locus_tag="PC399_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2594624.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         5826 a         2000 c         2967 g         5331 t
ORIGIN      
        1 ttgttgaaat gatgaaaata tatttgattt catacaaaaa agagtacctc ataactaaaa
       61 agttcacttt gagacactcc atttttatat gttagttgca tttaaggtaa aatagaagtt
      121 taatcatgtt gaaataactg aattacatct tctaaatgat tgtacattga tgtataacct
      181 aagtaagagt ctttactatc aaatgcaatc attatcttgt tatggtggct aatcatatcc
      241 ttgagagaat ttctatattt aaaaccaaag tattggttag aagaaactgt tttaggtatt
      301 aactcataat attttataaa aattggatta tgagaagcta aaattatctc tttgtgaaat
      361 tgcaagtcag catcataaca ttcttctatc aaaccgtgag aaaccttatc caattcttct
      421 attgacttta ggtaagtata ttttattttt tgaagttctt tagatgtagc atttaaaatt
      481 gctagtttag tagcttcact ttcgataagt tgtcttgctc ttaaagattc tacataaaat
      541 tcttgtatgt tatgattttt atagatttca ccatcatcaa ataggtcaac ttttttaaat
      601 ccttcaacta taggacttga ataaggcaca gctctaagta ttccttcctt acagagtata
      661 tttatggcat ttcttacagg tattctactt atgttaaata ggtcagcaat ctttctctca
      721 cttggtaatt tactattaat tttaaaagtt ccatttagaa tgtttaattt aatataatca
      781 ataatttttt cagtggcatt taagcttttc ataaaaactc ctaaacatat aattttttta
      841 tgattgtatt atatcatagt taagagttaa atctactttt ttataatatg tataagacag
      901 gatagaggcc aaaatagcta taatagccaa gataaagtat ccatattgta ttgaagtata
      961 ttccgatatt ttaccaacta tcattggaaa tacagacatt cctatagcat aaattgcttg
     1021 aaaaaaaccc attgcagtag atttctgatg ttttggtaca tttttcatag actcgctagt
     1081 aaggtaagaa aggagtatac ctgtagatat acctggtagt acttgtagta caaatattat
     1141 attaatattt tttattgttg gaactaaaaa gcaataggct gaaactaaaa taaaaactaa
     1201 aggaatccaa aattttggcc catacttagt acaaaattta gtagaagcaa agccagaaga
     1261 agcaacagca cacaccatgt aaaatataga agatagacct atgagggtag aactagaacc
     1321 taagttttta agaatttgag tagtaaatga cattgttgta gacatttgaa taccctgttg
     1381 aattaatgcc ataaaggaaa aaaatacaag tcttttatcc ttgcagatag ataaatattt
     1441 tttaattgat attttttcag gtattttttt tgaattatga ttttttatat ttaaggctag
     1501 aatcgttcca ataactgaag acaatacact catgatacat aaggctgtca ttcctaattt
     1561 ttcataaaat aaagtactca taacaaatcc aatcaacatt cctagattgt ttactaatat
     1621 tattgtactt gtagcttttt gttgatgttc tgtagaaaaa aagttggtat agtgaaccat
     1681 gtaagaaatc caagttgatg atgatattcc agataaaatg ttagctataa gaaaaccatt
     1741 gcctccagga gaaaagactc ttataagaga tgctaaacca gcaaagaaaa ttccagacag
     1801 tataaatatt ttatgtttat ttatggaatc agcaaatact ccaacaggaa gccttaataa
     1861 acattgtgag attccatatg cacctattat tataccaatc atatttgtag atacattaca
     1921 tgcacttaaa tatggatttt ggaaaggtat atatacatat tgtgaaaacc agaaaaatgt
     1981 aacaatagaa agtaaaagta atttttgatt agatattttt ttcatattat cctccgtatg
     2041 gtatttttat aatgcaaata atataccatt tggatttttt aatcaagtac tttctctatt
     2101 aaaaaaaatt taaatatata attattattt acaaaaaaat tatactttat agttgaatta
     2161 agaaaacgct tatgatacta taaagttaac aaatttgaat atataacagt agacgtgtta
     2221 ctggtaatgc aggcaagatc gaatcaattt ttatagatta tctatagagt actatgggta
     2281 taatctattt atttggttcg atttttttgt ttaattggcc ataaaacaaa aagcctatag
     2341 ttgaaaatgg aggatttttt atggattata aaaatatagc tcaagaaatt ctactaaatg
     2401 taggaggaaa agagaatgta aatgaagtga ctcattgtat gacaagactt agatttaaag
     2461 tcaaaagcgc ttctaaagtg aataaggata aattatctaa aactgaagga gtaataactg
     2521 ttgttgagag tatgggtcaa atacaagttg taattggaaa taaagtaaaa aaagtatatg
     2581 atgaggttat aaaaattgta cctcaatcaa ataacgtagg taaaaagaat gaaagtcaag
     2641 aaaagcaagg tataattaat tctatattat cagcagtagc tggtatattt acacctacaa
     2701 tacctgctat agcaggggtt ggtatgatta aaggtatttt atcagttctt gctatgtatt
     2761 atatgaataa aaatggagta gatataaaag aaacgcaaag ctatattatc ttaaatgcca
     2821 tggctgattc tatattctat tttatgccta ttatactagg atatactgcg gctaaagtat
     2881 ttaatgcaaa taaaataata tctatggtat taggagctac actttgttat ccaactttta
     2941 ctgctttgat ggcaggtgaa gaatctgtta gatttttagg attggcagta acaaaagcta
     3001 cgtatacatc atctgttatt ccaataatta ttgctatatg ggctctttca tacgttgaaa
     3061 aagtattaga aaagtatata cctgaggtaa ttaaaataat aatggttcca actttatcat
     3121 tggtagtaat gttacctgca acattatttt tatttggacc aatcggaatt tatataggaa
     3181 atgtcataaa cttttcttat aagtatattt atgaattaag cccagcattg tgtggagcat
     3241 ttgtaggtgg gttatggtgt gtgttggtta tatttggtgc tcatagagca ttattaccaa
     3301 taggtataag tgatgttgct caaacaggaa gacagaattt attggcattt gcaggagctg
     3361 caaacttttc acaagcaggg gctgctctag gagtattctt taaaactaaa aatcaaggac
     3421 taaaaacaat ttctatgtct gcaactataa cagctttatt tggtataact gaacctgcaa
     3481 tatacggtgc caatcttaga ttaaaaaagc ctatggtatg tgccgtaata tgtggtgcat
     3541 ttggaggtgc gataatggga atgggtggag cttatggtaa tgcatttgca aaccaaggtg
     3601 ttttaactat acctgtatat gctgaagcag gtgcattagg tttcttaagt tatctaggtg
     3661 gttgtgctat agcatttttt ggttcagcta tttcaactta tctagttgga tttgaagatt
     3721 tggaagagtc ttctaataag gaaaattcat ctataaaagt agaaacgaag gatggtgtta
     3781 ttgatataac ttcacctgta gagggcgaat gtatagaatt gtcagaagta aaggatgatg
     3841 tatttgcttc aaaagctatg ggagaaggta tagctgtgtt acctacaaaa ggagtaataa
     3901 cagcccctac tgattgtgaa gtagcttctt tatttccaac attacatgca ataggattaa
     3961 aattagataa tggagcagaa atgttaatac atgttggaat caatacagta gagttaaatg
     4021 gtaaatactt taccaaacat gttaatcaag gagatttagt gaaaaaaggt gataaactta
     4081 tatcgtttga tatagataaa ataaaaaagg ttggttatga tgtaactacg ccagttatag
     4141 taaacaatac atttgatttt gggcaagtag tttcttgtaa atctagttat gtttcaacca
     4201 atgataatat aatttcatta gtattagctg gtaattaagt taatttgact agagataagt
     4261 aaattgattt aaaaggaaat aagtaaatca acaattaaat acatctaaaa gtaagtttat
     4321 tgcaatgaat tgttaaagaa aataattaat atggagtaaa tctattgcaa tgaagtaaca
     4381 aagaaaataa ttaatataga gtaagtttat tgtaatgaat tgttaaagaa aataattaat
     4441 atagggtaaa tctatttcaa tgaaataaca aagaaaagag ttaatataga gtgaacttat
     4501 tgcaatgaat caacaaagaa aataattaat aaaaaggaga aatatttatg aatacaggat
     4561 ttccaaaaga ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg
     4621 ataaagacaa taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac
     4681 aggcagatac taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa
     4741 tgaaagaatt agggatgaaa acatatcgtt tttcaatttc atgggcaagg ataataccag
     4801 atggtgaagg agagataaac cagaaaggtt tagactttta taataagata atagacaaac
     4861 taatagagtg tgatattgag ccattcgtga ctttatacca ttttgacctt ccatttaaat
     4921 tagtagagaa atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg
     4981 ctaaaatatg ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac
     5041 agaatttaat tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga
     5101 agatagataa aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag
     5161 tcaatgcatg tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg
     5221 taacatatcc tcttacttct aagccagaag atatatatgc agctagaatg aatgataatt
     5281 ttaaagtgta ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga
     5341 agtatcttga aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta
     5401 aaaaagctaa aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt
     5461 tacctgaaga ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt
     5521 atggactatt taaaatgtca atgaatccta acttagaggc ttctgagtat ggagcagcaa
     5581 tagacccatc aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg
     5641 ttataataac agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg
     5701 atgactatcg tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag
     5761 atggtgtaga gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc
     5821 aaggttttaa taagcgttat ggacttgttt atataaatag aactgaccat gaaattttag
     5881 atttaaaaag aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg
     5941 gaatagtaaa ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat
     6001 taaataattc gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga
     6061 aaggcattgg ccataattat aaggccgggt ctgagttaaa tgaaaaagat atagaaaaga
     6121 tttttgttct tcatgatgaa aatataaaaa agagtattat acaattggca acagaaatag
     6181 atgcagaata ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact
     6241 taaaacttat gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac
     6301 gttttaaaga aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc
     6361 caaaagagta tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag
     6421 acctaccaga agaagagatt ggtaatatag caggccattt tataaatgca caacaagata
     6481 atccttatag tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag
     6541 ttcattatca cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa
     6601 tgcatctaaa ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag
     6661 attttatata tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg
     6721 taggagctta tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt
     6781 tgatgattca tattcataag attttaggag aattatgcga aaatagttag aatatcttaa
     6841 tatgctagta atattataaa aaattaaaaa ttaactgcac tttaaatatg aataattgca
     6901 ttacttaagg gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt
     6961 ttttaaacct ttatatcatt acaactttaa tgtttatacg cctgtacttc agatgtatat
     7021 ctacttttta catttctcaa catgcaaatc taccattctg tatatagtaa atgtattatc
     7081 atgtttgttt tattcaagca cacttataat acgcttgatt ttttcacaat gatgaaatat
     7141 actaatgggt gttataggta taagaatcgc attacacttg aagaagtaag tgataaatga
     7201 gatataaatc ctcatatgat aaactactat ttcattgagg aacatattct aagtgatgtc
     7261 aagatactac aatatgacta aattcctagt gataaaaaag tctacgaatg agtgtagaaa
     7321 ggggaactaa gatgaataaa gttttaatta tagatgatga taaagatctt tgtattttgt
     7381 taaaaaagag tatttcaatt gaacagattg aagcaaacta ttgccattct gggagagatg
     7441 gactggttga actttcaaaa caagattatc agttagtagt tctagatgtg atgatgccag
     7501 gtatggatgg ttttgaaact atggaacaaa tacgaaaata tagcagtatt cctatcttaa
     7561 tgctgacatc aaaaagtgat aattacagca aagtgcatgg attgcgagct ggtgcagatg
     7621 actatctaac caaaccattt gaaattacag aatttactgc tcgtgtactg tctttaatcc
     7681 gacgttacac ccgttttaat ccaaaaaatg aggagccaca gcaactttct ttcaaaggtt
     7741 tgttgattga ccttgaccag catacagtta ctaccaatgg cgaacctatg ttgcttccac
     7801 ccaaggaatt tggtgtactt ctttactgtg caaggcatca gggtaagatt ttgacaaaac
     7861 agcagattta tgaagccgtg tggggtgagc catatgtata tgatgacagc aatatcatgg
     7921 ctattatcag tcgcctaaga aaaaaaattg aactagaccc atcatctccc atctatttgc
     7981 aaacaataaa aggaattggg tatcaattca acacggaggt atgaaaatgg atgaacaaac
     8041 gataattctt ttcttactct tgctgtcgat tggtctactt tttctttcca tatttgcttt
     8101 ttccaagctt gggcatattc acaaacgctt aaaagatata gaggagatac tcgctgatgt
     8161 acaaattgga gacgaaaatc gtaaaatact aattaagcca tgtgatgtta tggctccatt
     8221 ggtttatcag cttaatgaga ttgtctacga ttatgaaaat aagttacttt ctctgaaaaa
     8281 gtcggataaa gctagcaaac aacttatgac aagtttatct catgatgttc gtacaccatt
     8341 aacaactctg attggttatc tggatgctgt acacagtggt atagttatcg aacaagagcg
     8401 ggaggaatac cttgaaatcg ctaggcgtag agcctacgac ctcaaggatt acattgatgt
     8461 tctttttgac tggtttcggt tgaactctga tgaatttacg ctgtcaatag aatctgtgga
     8521 aattgcagag ttgtcacgca atattttgaa agactggatt cctatattcc atgaaaagaa
     8581 actgaatttt gaaattgata ttccagaaaa tcgactaatg gtaaacctcg acccagatgg
     8641 atattcccgt gtggtcaata atctggttca aaatgttctt gctcacagta aggctcggca
     8701 aatcaagatt accatgagtg aggattccag aatggtatta ctccgtgttg aggatgatgg
     8761 tgttgggatt gccagagaaa atttaccaca tatttttgag cgactgtata agtgtgataa
     8821 aggacgttca gaaaaaggaa gtggtcttgg tctatctatt gtcagtcaaa tggtggagcg
     8881 aatgggtggt caggtttccg tagaaagtga gattggaaag tataccgtat ttacagtgag
     8941 cttaccatta atataaaatt gtttttcccc catgtgacat gtatacacag ggggattttt
     9001 tgtaagttat aaagttgcaa ggttaataca aggttgttgc aaggttaatg taagattgag
     9061 gtggtatgat aactttaata aacaaaggag gcttttatta tttatgaata atttaattat
     9121 tgaaacaaaa aatctcacta agcaatatgg tgctcaaaag agcgtttcta atttgaatat
     9181 tcacgttcag aaaggtcgca tttacggatt gctaggaaga aacggtgcgg gtaagacgac
     9241 aactatgaaa atgttattgg gcctaacaaa accaactact ggagaaatta aaatatttgg
     9301 tcaggacatt cacaataacg agaagaagct tcttcctcga attggatgtt tgattgaatc
     9361 accagggttt tatcctaatc tgacaggaac agaaaatctc aaaatctttg caaggcttag
     9421 aggaatacca aaaaaaaatg cagtagaatc agcactggat gtagtcggtt tgccaaacaa
     9481 tgacaaaaag ttattttctc aatactctct tggtatgaaa cagaggttgg cgattgctct
     9541 tgccattatg catgacccac aactgttgat tttggacgag cctatcaatg gacttgatcc
     9601 tattggtatt gcagaagtaa gaacgtttat tcgtgatctc tgcacagaac gaggtaaaac
     9661 tattttgatt tccagtcaca ttctttctga aattgccttg ttggcagatg acattggcat
     9721 tattgaccat ggtattttgt tggaggaaga aagtcttgcc caattagaag aaaaaaacag
     9781 taaatacata cattttgctg tatctgatgc agcacaggca gcaaggcttc tggaaaccca
     9841 acttaacctg aaaaatttca aagtagcaga taattacaat attatgctat atgatacaga
     9901 tgtttctgtt gcagaaataa atcgtgcttt cattctaaac aacattgagg tatcagaatc
     9961 tcatttatgc gaagatactc ttgaagatta cttcaagaaa gtaacagggg gtgaaggaat
    10021 tgcttgatat aatttcatgt gaattttcaa aactcaagcg gttaaaattt atactgattt
    10081 ctattttagg tgcttgctta tttccaattc ccgcaacaat tttgattgca aaggacaact
    10141 tgccctttga gcaattgttc aaattagttg tcaattttgg atatttcttg ctattaccaa
    10201 ttgtattaag tattgttgct agccaactat tttttataga aagagataat gatgtattaa
    10261 aaaatcttgc aacagtgcca gtgccaaaag gaaaactggc tctggcaaaa ttggctgtgt
    10321 tgctgtttat atcattgttc tactctgttg cagggttagg agctactatc attggaggtt
    10381 tcattgttgg aatagtagag ggggttgctg taaaattagg aatgagcatt gcttttggaa
    10441 ttatgttttt tgttactgta cttcctgttg tggtactcat tgtatatttc aatcgcagct
    10501 atattttttc tattatactt tcttttgtat atgctatttt caattttagt atatccttaa
    10561 atattatcaa ttttgaacca aataacccac tattaagcgt attacctgct cctgttataa
    10621 tgcgttggtg gatggcattt tggggagatc ccactggcga atacacggta cttagacagc
    10681 catatttgct ctcaactcct gcttgtgtag gtattttatg tttgataacc attatagcag
    10741 tcctgctgat ttgtatgata tacaaaaagc aggagaatta ggaggtatct atgcttgata
    10801 taattactac agaatttcaa aaaataaaaa gatataatat tctatggatt ggtgttgttg
    10861 ctgttctgtt ttcagcacta ttggcagttt ttcaggttgt aagttcacac ggcagtgacc
    10921 ctctaacgta tgagagcttt gcaaacggag ttatctggaa taatttcagt cttagttttc
    10981 catttgggat tacacttatt ggaggatatc ttattaaccg tgaatataca gaccaaacct
    11041 taaaaaacat cctaactgtt cctatttcat tacgcaaatt gctagtggga aaattaattg
    11101 cggttggagg aattactgta ttgttaagtc ttttcagctt cttatgcaca tttattttgg
    11161 ggttgttatt ttgcaatatc aatatatctg ttatactcgt tgtaaaatcc ttagttcaga
    11221 ttttgtctgt taatctgtat tgttatgttg ctgttttgcc gattattgct tattttggtc
    11281 gtaaacaaaa tgcatttctg acgggtgtag ggattgcatt tgtatacggt ttttgtggtg
    11341 tgtttgttgc tggaagaaat ctgacagact tttatccaat cacagcaggt cttggtctag
    11401 ttggatacaa caatggtgct ggtactgttt atcagccact tatcggtgct atgactttga
    11461 ttgtaattct aatcttgaca actattctac ttgtatttac acctaattat gataaagtta
    11521 tgaccatatc taagaaaaaa gggataaagc ataaaaagta ttctcataaa taacataaat
    11581 attgtttcta ttatttaagt tgctagacag tgaaaagaaa aaatctgtcg tacagtggct
    11641 atatgctcat gtattcaaga agctacagtt ttgatttttc ataactgtag cttctttttg
    11701 tatttattaa atcacgacta tcataatact tgcaataggt atagtaacaa gagagagtac
    11761 tgtcatatgt gttttatata ttacattaag tatattctaa aatatatgtt ttaaataata
    11821 tatttgaaaa taggaatatt taaataaaaa aaataatatt atatatagtt gacaaaaatt
    11881 ggcaaatgat atactatata aatatagaaa tagtaatcat tactattatt aaaaagggag
    11941 gaattaatta tgaacttaaa aggaactaaa acagaaaaga atttaatggc aggatttgca
    12001 ggagagtcag aagcaagaaa taaatataca tactacgctt caaaagcaag aaaagaaggt
    12061 tataatcaaa tagctgctat atttgaagaa actgctaaca atgaaaagga acatgctaaa
    12121 ttatggttta aacttttaca tgatggtatg ccttcaacag aggaaaattt aaaggatgca
    12181 gcagcaggtg aaaactatga atggactgat atgtatgcaa aatttgctaa agaagctaga
    12241 gaagaaggat ttgacaagat agcttattta tttgaagctg ttggaaaaat agagaaagag
    12301 catgaagaaa gatacttaaa gcttttagaa aacttaaatg aaggaaaaat atttaaaaga
    12361 gatgaagaag ttgtttggca atgtcaaaac tgtggtcatg tgtatgtagg tactgaagca
    12421 ccagaaaaat gcccagtatg tgaccatcca aaagcatatt ttaatataaa agctgaaaat
    12481 tattaaaaat tatcattatt aaaaaggaat gttaactatg aaattttcta aacaacgaga
    12541 actgatttta aatgaaatat taaataatcc ggttcatcct actgcggatt acctatatga
    12601 aaacttaaaa aaagataatc cgaatttaag cttaggaact gtgtatagaa atttagctca
    12661 gctaacggaa catggcttta taagaaaggt tagtattcca gggtatccag atagatttga
    12721 tggtcgaata gataatcact atcatatcat atgtgaagta tgtggagagg tatacgattt
    12781 agaatccgaa gttcttaata atttacaaga attaatatct gaggagacag acataaaaat
    12841 aacatcttat aacataagtt ttaaagggat ttgtaataat tgtaaaaggt gtagccaagt
    12901 aggttagttt tcaaattcta aaaataatta atgaggtgag tttatgtgta gtgaacaaaa
    12961 attttttata tgtgagactt gtggaaacct tgtaggaatg atacaaagtg gtggtgtacc
    13021 tattttctgt tgtggaaaac ctatgaagga acttgttcct aatacaacag atgcagcagt
    13081 tgaaaaacat gtacctgtaa ttgaagtgga tggaaataat gttactgtta aagtaagtag
    13141 cacaactcat ccaatgacta aagaacatca tatagcatgg gtgtatctta tgactgaaca
    13201 aggtggtcaa cgcaaatgtc tagcagtaga tggagaacct gtagttaaat ttgctttgaa
    13261 tgatgatgat aaagtgattt ctgcctatgc ttactgtaat ttacatggat tatggaaggc
    13321 tgaactgtaa tatttaaata aattgagtca aaaaggaggc tataaatgta ttttcataca
    13381 aatagcctcc tataaattta tataaattca agttttaatt ttgtttaatg tcaatataat
    13441 ataaggagaa aataatatgt caatttataa atgtagtgtt tgtggatata tttatgatga
    13501 atctaagaat gataaaacat gggatgaatt aagtgaagat tgggaatgcc ctgtatgtac
    13561 aaaaggtcgt agttattttg gaaagataag tactgtttac tatgaagaag atgaaaagat
    13621 agcagaagac atagttgaag atgaatctaa attaaatact gaaaaagagg gagatttaaa
    13681 ttacttaagt acatatttaa gaagagatga tgaagttgag aaacatatgg atataattca
    13741 cgaaatggct gttacaggaa aatctataat agaacctatg agaactaaac taccagtaat
    13801 ttcttgggat gatattttaa ttatgggtgc tcaattaaat ccattacctt taaatgagca
    13861 tgatgaagta aatacaacaa caataatagg aaaaaaagca aagaaaccta tgattataga
    13921 aaatccagtt tatatttccc acatgtcatt tggagctttg tctaaagaac taaaaatagc
    13981 tttagcaaag ggtgcagccc aaaataagac agctatgtgt agtggtgaag gaggtatatt
    14041 accagaagag aaggaagctt catacaagta tatttttgag tatgtaccaa ataaatatag
    14101 tgtaactgaa gaaaatctta aaaattctga tgctatagag ataaaaattg gtcaaggaac
    14161 aaaacctggt atgggaggtc atcttcctgg cgaaaaagta actgaagaaa tagcaaaagt
    14221 aagaaataag cctgtaggtc aagatgtaat aagtccatct tgttttgagg aaatacaatc
    14281 caaagaggat ttaaagaaac ttgtagatga acttagagaa gtttcagaag ggcgtccaat
    14341 aggtgttaaa atttcagcag gtcatattga aaaagatatg gaatttatag cttatgctaa
    14401 acctgatttt gtaactattg atggtcgtgg tggagctact ggagcaagtc caaagttgct
    14461 taaagatgca acttcaattc caacaatatt tgctctttat cgtgctagaa aatacataga
    14521 tacacatgga ttagatatag atttggttat aacaggtggg cttcgtatat ctactgattt
    14581 tgctaaggca attgctatgg gggctgatgc agttgctatt gcaagttctg ctcttatggc
    14641 agcggcgtgt caacaatatc gtatatgtgg ttctggtaag tgtcctgtag gagtagctac
    14701 tcaggatgag gaattgagaa aaagattaca tatagaaaat agtgctaata gagtggcaaa
    14761 ctttttaaat gtaagtttag aagaacttaa gacttttgct agaatttctg gtcataaaga
    14821 tatacatgat ttaagtgttg atgatttata tactgttaac tctgaaatat caaattatac
    14881 aaacatacag catgtataga ttgaagtttg aaactattag attttagaca gctataaaag
    14941 taaatgataa atatatttta taaaagagag tgtcttaaaa tgaacttttt agttcataag
    15001 tcactctttt ttgtatgaag ccaaatatat ttatcatttt agcaatgaaa aagaatctta
    15061 tctctttatt ttgagataga ctccttctat ttatgaaaat taaagatatt aaattgtaaa
    15121 atattctgtg atgtcgtact attaaactaa agattctgta ctttaaagta aataaaaatt
    15181 taaggagtga ttattatgga aagattgaag atacaagttt tagtcgacaa taatacttat
    15241 attgatagat actttgttgg agaaccagca gtaagctatt atattgagat tgatggaaat
    15301 aggattttgt ttgactcagg gtattcagat gtattcatat caaacgctga aaaattaaat
    15361 atagatttag gaaatttaac tcatgttgtt ttttctcatg ggcataatga ccatacaaga
    15421 ggtatccagt ttttagaaaa taggtatgat ttatctacag ttgaacttat ttcacatcct
    15481 aactgtttta tacctagaaa acatggagag aaaagcatag gagctccatt ttctgctgag
    15541 gaaattaaaa atatttttat gtataatcct aaggataagc catttaatct aagcaagaat
    15601 tgtgtgtttt taggtgaaat accttctata aatgattttg aaaaaagagc taaaattggt
    15661 aaatgtaaaa taggagattt gtgggaagat gattatgttt tagatgattc tgctatagtt
    15721 tgtaaaactg ataaaggtat ttttattgtt acaggatgtt ctcatagtgg aatttgtaat
    15781 atagtagagt atgctaaaaa ggtatgtgga gatgatagag ttattggtat actaggtggt
    15841 tttcatcttt ttgaattgaa taataggtta gatagtacaa tacaatattt ggataaagaa
    15901 gaaataagaa tgttgtaccc ttgtcactgt gtttctttta aggtaaaagc gaagatgaat
    15961 gaaatattga atataaatga agttggagta gggctgacca taagtatata gtatttgaaa
    16021 gtatataaga tttaagttga aggttaaaaa gatattttct atagaaaata tcttttttgt
    16081 tacgtttatt ttaagagaaa tcaatatact tttatatgaa atat
//
LOCUS       DAMJWS010000243        15199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.guided.097, whole genome shotgun sequence.
ACCESSION   DAMJWS010000243 DAMJWS010000000
VERSION     DAMJWS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.guided.097"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="PC399_003880"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="PC399_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2594625.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="PC399_003881"
     CDS             1575..2258
                     /locus_tag="PC399_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2594626.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="PC399_003882"
     CDS             2255..4915
                     /locus_tag="PC399_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2594627.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="PC399_003883"
     CDS             complement(5096..6277)
                     /locus_tag="PC399_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2594628.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..6939)
                     /locus_tag="PC399_003884"
     CDS             complement(6277..6939)
                     /locus_tag="PC399_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2594629.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(7071..7709)
                     /locus_tag="PC399_003885"
     CDS             complement(7071..7709)
                     /locus_tag="PC399_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2594630.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7888..8493
                     /locus_tag="PC399_003886"
     CDS             7888..8493
                     /locus_tag="PC399_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2594631.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            8997..9746
                     /locus_tag="PC399_003887"
     CDS             8997..9746
                     /locus_tag="PC399_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2594632.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            9743..10507
                     /locus_tag="PC399_003888"
     CDS             9743..10507
                     /locus_tag="PC399_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2594633.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            10606..11427
                     /locus_tag="PC399_003889"
     CDS             10606..11427
                     /locus_tag="PC399_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2594634.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKNILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDVVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            11441..13105
                     /locus_tag="PC399_003890"
     CDS             11441..13105
                     /locus_tag="PC399_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2594635.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            13110..14984
                     /locus_tag="PC399_003891"
     CDS             13110..14984
                     /locus_tag="PC399_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2594636.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
BASE COUNT         5709 a         1748 c         2526 g         5216 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtattacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaaggc gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atataaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataag
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggatatag aatcttaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcagttagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctatagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggc tgactgaaaa agatgcatca aaagaacttg cgaaaaatgg
     3901 aggatttaaa agtatagttt catctaaaaa atactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttataa tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtacagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaagtaa
     5701 ttggagtctt aagttgatga gcaatatctg caagattatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatat aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ctgtcctcca ttctatatcc
     6301 tatgcctctt aacgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggactgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca tcatcttcta aaagaagtat tttagccatt tacttttaat cctttcagta
     6961 atttagttat tccattaact aagttattag ttttatgttt aatatttttt atattagaga
     7021 tagaaaaaat tatcatttaa gtatataaac tttaatttat aaacttaggt ttatgcttta
     7081 attgaatcta ttaaaagagc aagagttgtt tcaataaatt tttcctcatc tatattaaag
     7141 ttgttcaggt atgattttcc tgttacagat agtaaattaa aaaatcctgt aagtgtaaat
     7201 attgatgaat acataagagt aggtatttca atatcatgcc taatacttct atcatttttt
     7261 ccaatttcaa acattccttg tatttcatca aacataaatt tattaaaaga aaaaaacttt
     7321 tctttaaaca gcatatcagt atccgatttt tcagagttta caataccaat cattcccata
     7381 aggcataata gagaaggttc tacagagtaa aatttatgaa aagcaaagta tgaaagtttt
     7441 attttttcaa accctgtttt gccttttgca ctttctattt taacattatc ccaaagtctt
     7501 ttatatcctc ttaagacaac agcaaagaat aaatcttctt tacatgaaaa gtacttgtat
     7561 atggtgcgtt tagtatattc aacttctttt gctaattcat tcatagttgt attatcaaat
     7621 ccattaagac aaaataattt ttctgcttta tcaataatct cattttctct ttgtattttt
     7681 tctttatctc tttttgataa ttctgacata taaattattc ctttctattg ataaaaaagt
     7741 atactgatgg tatacttatg taaatataat acttgtatac ctatagtata ctttaataaa
     7801 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
     7861 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
     7921 agtactcgtg gctcaaattg ttttattgtg tttgataaag aaattattct tatagacaca
     7981 ggtcttcctt ttatggggaa aaatataatt aaagaattaa aaacacttgg cattaaactt
     8041 acagatatta agcatatttt attaactcat catgatgttg accatatatc taatattaaa
     8101 ttattgaaag aaaaaacagg agcaaaagta tgggctcatg tagaggatat accttttatt
     8161 ataggagaga aggatagacc tgggtttaag aagtttattg gaaaggcaat ttccagaagg
     8221 ctcattaagg atgttgaacc ttatggagag aatatgcaga ttgggaatat aaaaattatc
     8281 catactcctg gtcatactcc agggcatgtc tgcatgattt ttgaagctgt attatttgca
     8341 ggtgatttag tcaaaaataa gaatggaaat atagttcctt atcctaatcc atggaattgg
     8401 gattataaaa agatggtgaa atctataaga gaattggata atttaaaata tgaatggata
     8461 tgtatgtctc atggaactcc ttgtattaaa taataaaaat taacatggat taatagaggc
     8521 atagttgctt gtatgtatga taataattta cagcttcttt ttaatatatc atgtatattt
     8581 gtagctgtaa gcatatttat aacaatactt acaattttgt attttaacat ctataataat
     8641 agttttagct ttgttaacta tgaaatttac ttaaagaagt aaatattcaa gtaatagtaa
     8701 ttattaaata agataaaaag taaatggcag atgaattaaa gtaatttcta ccatggaaaa
     8761 tacttttgag ttagaaaaaa ctatatttta gagtatgaag agatgaagct tccgaagaaa
     8821 gcaatatcta aagtacatgt tcgaaaggat aagtactttt tggtgcctgc ttttagcagg
     8881 ttatttttat tttaataaaa ataaaatcaa atgattattt tatccttatt atttaaaact
     8941 cttccctaga aaaagcaact aatgtatatt aaaataatat ttagggagtg gaaaaaatga
     9001 gattagaatc gttagttaac caaaattatg ataatttaaa tgaaaatgat aaaattatta
     9061 ttaaacaaat attgatttgt caaagagaat accgtaattt ttcgtgtgaa aaaattgcaa
     9121 atgaatgtaa tgtttctaga gctactttat taaggctttg tagaaaaatt ggattagatt
     9181 cattctctga tttaaagtat ttacttaaaa ataatcaact agaatttgtt aataatgata
     9241 ataattttca tgatgtttgc gagaattatc gactattaat gaatgaatta caaaaaatat
     9301 catttcaaga tatttgtaaa ataatatatg atgcagaaac aatttatatt tatgggacag
     9361 gtaatgaaca gaaaagttta gctcaagaaa taaagagaat atttctttca gtaggtaaat
     9421 gtgttattga cttatttgat ataggtgaaa ttgaatttat gagagaatca tttcaaaaaa
     9481 atgatttatt tattatcatt tctttgtctg gagaaacaaa agcaggaatt gaaattgcaa
     9541 aatttatcaa caatcatata catacgattt ctttaactag gcttgataat aatacaattg
     9601 caacactttg tgagtataat ctgtatgttg aaacgcaaaa acttgataca ttacaagcta
     9661 tatcttatga agtcgtagga gcattttatg ctctattaga tttgcttcat atgaattaca
     9721 tagagtatag aaggagaaaa gaatgaaaat tgaaggactt ttcaataaat attataattc
     9781 atttacagca aatgataagt atatctgtga gtgtattctt aatcataaag aagattgtat
     9841 taaactttct atagatgaat ttgcagacaa ataccatata tctacaagtg cactttctcg
     9901 ttttgctcaa aaactacaat tacctggata tagtgaatta agagttatta tacgcttaaa
     9961 tgaaacagaa gtagaatata aaaatcaaag taaacaccaa ttaatggatt gttataataa
    10021 ggtgatagat gatatagaga agaaggattg ttcgattatg tttgaaagaa tgaaaaaggc
    10081 aaatcgtata atcgtctttg gagaaggtta ttctcaggaa agagttgcta aagagatgaa
    10141 aaggattttc ttaccaacag ggaaaattat atatgatgtt tatggttatg atatgattca
    10201 ggcacttaat cattttataa aaacagatga tatgattttc tttatttcat tgaatggaga
    10261 tgcatctaca attattgatt ttgctaaaga aatgagaatg aaaggaattt atatggtttc
    10321 tattacaaaa atgataagta atcctttagc aagattatgt gatgaaaacc tgtatattca
    10381 atctatatct atttctgtag ataaacaact ggactacgat gtaacaacac cttattttat
    10441 tttaattgaa ctgctttata ttaaatacaa gatgtattta gaaagtgaac atattccccg
    10501 aaaatgaaaa tttgtgctaa aaatgaacat attttttaaa agatatcaaa atattgatat
    10561 cttttttttt gaatgctatt atgattttga tagtaagaaa ggaagatgaa attgtacaaa
    10621 ttattaatac ttgatataga tggtacgctt agagatgagg tatatggaat cccagaatct
    10681 gcaaagcatg ctatacgttt atgtcaaaag aatcattgca gtgttgttat ttgtactgga
    10741 agaagtatgg gtacaataca agatgatgtt ttatcattag gagttgatgg atatattgca
    10801 ggaggaggta attacattca atatcatggt gaattacttt ataatcaatc ttttaatcaa
    10861 agattgataa aggaagttgt ttgtttatta aaaaagagag aagttgcttt ttcgattgag
    10921 agtcaggaaa aagttttcat gaatcaaaaa gccaaagaaa tatttgaaac aatgaatcag
    10981 ttgaaaggaa ctaattcttg tataaataaa caacatatcc aagaaaaaat aacatatgaa
    11041 aataacattg aagagtataa gtcacaagat atacataaaa tttgtttatg gtcaaatgaa
    11101 aaggtatttg acgaagtgaa aaatatacta caagataaaa tggaattagc acagagagat
    11161 atttctagtc agtattatga aattattcaa aaagattttc ataagggcaa agcaattaag
    11221 agattacaag aaagattagg agtaactcaa aaagaaacca tatgttttgg tgatggtcaa
    11281 aacgatgttg tcatgtttca agcatcagac gttacaattg caatgaaaaa tagtcatcaa
    11341 caacttaaag atatagcgac atctatatgt gaggacatat tcgataatgg aatttataaa
    11401 gaattaaaaa gaagaaatat catataaaaa ggagtaattt atggaaaaga aatggtggca
    11461 aaaagaaatc gtttatcaaa tttatccaag aagctttcaa gatagtaata atgatggaat
    11521 tggagattta cagggaatta tttctcgttt agattattta aaaaatttag gtattacatc
    11581 attgtggtta tgtccaattt ttaaatcacc tatggacgat aatgggtatg atattagtga
    11641 ttatttggat attgcaagtg aatttggaag tatggaagat ttaaaagagt taattaaaga
    11701 aagtaaagat agaggaatca aaattatatt agatttagtt cttaatcata caagtgatga
    11761 acatccttgg tttcaagaag cattaaaaaa tccagaaagt ccttatcatg attattatat
    11821 ttttaaaaaa ggaaacgatt taccaaataa ctggaggtct gtttttggtg gaagtgtttg
    11881 ggaaaaggta gagaatagag acgaatatta ttttcattct tttggtaaaa aacaacctga
    11941 ccttaattgg gaaaatccaa tattaagaag tgaactttac caaatgatta atacctggtc
    12001 tcaaatggga gtggcaggat ttagagttga tgctattaca ttcatcaaaa agaatttaag
    12061 ttttcaaagt ttagaaagtg atggtgttga tggtcttgtt aaatgtacaa aaacagttag
    12121 aaatcagcct ggaatagaaa aaatgcttta tgaattaaaa gaaaatacgt ttaaaaaata
    12181 tgattgtatg actgttgctg aagcagcagg tattagctat gaccagctag gtgattttat
    12241 tggagaagat ggtttctttt caatggtctt tgactttaaa catgctgact tagatgttgc
    12301 ttctggtagt gagtggttta aaagaatttc atggacaatt ccagatttaa gaaaatgcat
    12361 tttagatagt caaatgtatg ttcaggaaca tggatgggca gctaatttta ttgaaaatca
    12421 tgaccaacca agagctacaa caaagtatct tttgcaatac gaaaggaatg atgatgcagt
    12481 aaaaatgttg ggagcaatgt atttcttttt aagaggaacc ccatttattt atcagggaca
    12541 ggaattagga atgacaaatt ttaaaagaga taccataaat gattttaatg atttatctag
    12601 cattgaccaa tactatcgtt caatagatga aagttactca gaagaagaag cgttagaatt
    12661 tgtgaattta agaagccgag ataatgcaag aacgccattt ccatgggcag ataaagaaaa
    12721 tgcaggtttt tcagatacaa caccatggtt aaaggttatt gaaaattata aagatattaa
    12781 tgcacagagt cagattaaca attcagagag tattttagaa ttttataaaa agatgataaa
    12841 gttgagacaa gatagtgagt attctgattg tttgatttat ggttcaatag aacctattga
    12901 tatagaaagt gatgatatta ttgcgtatag aaggcaatta gatttggtag ttattgattg
    12961 ttattataat ttttctgata aacctgtaga aataagagta gaagaaaaat atgatattgt
    13021 attccaaaat ttagatgata taaatctatt aaataaaaca gtgcgtttga aaccatttca
    13081 tgcagtatta ttgaaagtga ggtagaaaaa tgaataaata taataaaata gcaaatgagc
    13141 ttattaaaat tattggtgaa gataatatta tttctattac acattgtgcc acaagattaa
    13201 gagtgatggt taaagataga gaaattatta atgataagaa agttgaaaaa gttgatgagg
    13261 ttaaaggggt tttctttact tctggacagt atcaaattat tttaggaaca ggaatagtta
    13321 ataaagttta tgcagaagtt gaaaaaatgg gattaaaaac tttatcaaaa aaagaacaag
    13381 atgaattagt taaaaataat gaaactggat ttaagaaagt tatgagaact ctagcagata
    13441 tatttgttcc tattattcca gttattgcag caacaggatt attcttggga ttaaagggtt
    13501 gtttgtttaa tgataatgta cttggattat ttggaatgtc ttctgcaaat attccattgt
    13561 atattcagac acttgtttcg gttcttacag aaacagcatt tgctttttta ccagcaatta
    13621 ttgtatggtc agcatttaaa gtatttggag gaacacctgt tattggactt gttattggac
    13681 ttatgttagt ttccccaata cttcctaatg catattctgt tgctgaccca tcaaatgaaa
    13741 ttgaagcaat tatggcattt ggatttattc ctattgtagg ttgtcaagga agtgttttaa
    13801 cagctattgt tacagcattt attggagcta atcttgaaaa atggttccgt aaacatatgc
    13861 ctaatgtatt agaccttatt ttcacaccat tttttgttat gctaataaca atgttagtta
    13921 ttttgttagg tgtaggccct ataatgcata caatagaatt aaaaatggtt gatatcatca
    13981 gcttattgat tgatttgcct ttaggaattg gaggatttat tattggattt acatatccat
    14041 tagctgttat tactggctta catcatactt atgtaatgat tgaaacatca ctattagcaa
    14101 atacaggatt taatgccctt attactcttt gtgcaatgta tggatttgcg aatattggaa
    14161 catgtctagc atttatgaaa aaatctaaaa ataatcaagt taaacaaact gcggttggag
    14221 caatgctatc tcaacttttt ggtataagtg agcctgtttt atttgggatt cagcttcgat
    14281 ataatttaaa gcctttgatt attatgtgtg catcttcagg tttaggagca gcaatattat
    14341 caattttaca tattcaatct aattcatatg gattagcagt tttaccttct tatttaatgt
    14401 atatctatga tggatataat ttaatcacat acttattagt atcaatattt gtggttgcat
    14461 tctgctttat tgtaacatgt ttatttggag tgcctaaaga agctataaat gaagatgaag
    14521 acgaagaatt agtgtttaat gaaaataatg aaaactttgt gtctccagct aaggggaaaa
    14581 ttgttgcatt agaaaatgta ccagatgaaa cattttctaa aaaaatgtta ggtgatggtt
    14641 ttgctattga tattatagat ggaaaaattg tttctcctat ttcaggtaaa ttagaaacag
    14701 tggtttcaag tggacatgca tttgggatta aaggaactaa tggagaagta ttaattcatg
    14761 ttggaattga tacagttgca ttaaatggag atggctttga tgttgctgtt aaacaaggag
    14821 atatggttaa acagggagac gttcttgtta atgttgattt aaagagaata catgaattag
    14881 gaaagtctac tttgacaatg gtattgtttc cagatggtaa gaaggtaaat attttggata
    14941 taaacaagga tgtaaaaata ggtcaaagaa tatgtgttga gtagtaggtg atatgtaatg
    15001 actacaaaag aagattatca agagtaattc aatcttttta aagtatgaga aataattcat
    15061 aaactctaaa acaattaaag aggttgtttc aaaatagaga ttgtattgca cccaaattgt
    15121 tgaaatgatg aaaatatatt taacttcata caaaaaatta aatagtggca acaattgagg
    15181 atgtgaggga gagttgcca
//
LOCUS       DAMJWS010000244        11305 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.guided.112, whole genome shotgun sequence.
ACCESSION   DAMJWS010000244 DAMJWS010000000
VERSION     DAMJWS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.guided.112"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            236..574
                     /locus_tag="PC399_003892"
     CDS             236..574
                     /locus_tag="PC399_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594637.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            596..913
                     /locus_tag="PC399_003893"
     CDS             596..913
                     /locus_tag="PC399_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2594638.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1063..1740
                     /locus_tag="PC399_003894"
     CDS             1063..1740
                     /locus_tag="PC399_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594639.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2068..2835)
                     /locus_tag="PC399_003895"
     CDS             complement(2068..2835)
                     /locus_tag="PC399_003895"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2594640.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(3507..4322)
                     /locus_tag="PC399_003896"
     CDS             complement(3507..4322)
                     /locus_tag="PC399_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDF2594641.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSSRAEMERL"
     gene            4414..5682
                     /locus_tag="PC399_003897"
                     /pseudo
     CDS             4414..5682
                     /locus_tag="PC399_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            5781..6401
                     /locus_tag="PC399_003898"
     CDS             5781..6401
                     /locus_tag="PC399_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2594642.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            6551..6955
                     /locus_tag="PC399_003899"
                     /pseudo
     CDS             6551..6955
                     /locus_tag="PC399_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            7080..8006
                     /locus_tag="PC399_003900"
     CDS             7080..8006
                     /locus_tag="PC399_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2594643.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            8020..8469
                     /locus_tag="PC399_003901"
     CDS             8020..8469
                     /locus_tag="PC399_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2594644.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            8829..9236
                     /locus_tag="PC399_003902"
     CDS             8829..9236
                     /locus_tag="PC399_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2594645.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            9238..9468
                     /locus_tag="PC399_003903"
     CDS             9238..9468
                     /locus_tag="PC399_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2594646.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            9858..10061
                     /locus_tag="PC399_003904"
     CDS             9858..10061
                     /locus_tag="PC399_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2594647.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            10139..10492
                     /locus_tag="PC399_003905"
     CDS             10139..10492
                     /locus_tag="PC399_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2594648.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            10564..11196
                     /locus_tag="PC399_003906"
     CDS             10564..11196
                     /locus_tag="PC399_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2594649.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         4138 a         1623 c         1787 g         3757 t
ORIGIN      
        1 catattatta attcgtttta ttattgtagt atggttatga aattatgaaa atatttctct
       61 aattaaaaat atgatttatc catatttgtt tattgtagat acagactata caaaaataaa
      121 tagcacttat ttcagtataa aaacatatat agtctatatt tagactaaaa aagtataaat
      181 agtctatata aaacaactta aactatgaat ataatctaaa gtggagggat aataattggc
      241 aaatatagga aaaataatag gagatagaat aaaagtctat agaacaaaat tgggatatag
      301 tcaagagttt ctagcagaaa aggcaggttt acatccaaca tatattggtc agattgaaag
      361 agctgagaca aatacatcta tcaatattat aatgaaaatt gctactgctt tagacatacc
      421 acttgaattg ttatttgcaa atattattac aacagaaggc gttgataact ctgtaccttt
      481 agaatgttat gaaatgatta ataaattaac tgaaaaagaa caaattgcaa tgctagagtt
      541 aataaagtgc atcattaaat acaaagaaat ataagtgagg gaaatagtaa caataatgaa
      601 ttatataggt aaaagactta aagaagaacg gaaaaaagca aatttaactt caaaagaatt
      661 tgcaaatatg gttggagtat cgccttggta tataactcaa atcgaaagtg gtaaaaagaa
      721 tccaagtctt aaaacattca taaaatttgt aaatatatta aatatttctg cagatgttct
      781 tataaaagat attacttcca ctggaaaaac atatttagaa aatgatataa atgaagagtt
      841 aaaagactta aattcaagag aactaaatct gatatcacag attatagaca tcataaaaaa
      901 gaataaaata taattcttta tattttatat aaaaaaaaag gttatttgaa ataaataacc
      961 tttttttgtc tttttgtgtt aacattgtct tatagtgcta atgagtacta ttagaccatg
     1021 ttctatggcc ttattttaat ataaaacgat tggagatagg ctatgattgt gattgaaggt
     1081 agcgataaat ttaagattgc aaaagaatat attgatgtag aatatactct ttttagcaaa
     1141 gtaactttta ggtatgaaaa gttgaaattt aaagataatg ctgaattgga aaaaattaag
     1201 atgtttaaat ataaaaatgg ctacatccct aataaattaa acctttcttt tggatatgga
     1261 ttctcttctt ataaaaagca aataattaga gaaactgtag atactttaag attgacagaa
     1321 attttttcaa gcgagaacat agaagatatt aaatttataa aagatggtac aaaaaaatta
     1381 gaaattagca tagagaaagt tgtgaaattt aaacgtcgaa aaaaaagaaa ttatgtttgt
     1441 tgctattgcc ctgatatgta tagagacata aaactcgaca aagaatctat caataagata
     1501 tacaacagaa aaataaaaat agaaagagaa gttaatattt ttgaagatga ggatgttata
     1561 ataaacaaga aagtattgaa gtttccaaag tcttggacaa agaatatgca aaaatattgg
     1621 ttaagtgaaa ataagtatcc catacattct actgtaattg atgatgatag atataaatgt
     1681 tgtaatgtac aatatacaaa aaatagagtg ataatattat attacatata taaccattaa
     1741 tatacataat tacaaaaaaa taatgacaaa tatacataat acaaatatat cttctacttt
     1801 agataaaaca gttattatga aaacttacta tctataaata atagaaatgc tagtatacca
     1861 tctataatct aatgtctagt aggttttaat ggcatatttt aaatatgcca tagttgattt
     1921 ataagatttt ctatatcact ttcaaaatac acactaaata gttgcttttc agatataatt
     1981 gcccaattta aattttacta tctgaaaagt tttttatatt taatttatat cttcaaaaat
     2041 accgtctaat atttctatat aattaattta attaatactc ttatgtaata gattaagtac
     2101 tgaatttaac tctttaaatg atatttgccc tggtgctgaa acactttttg cagctccaaa
     2161 agtcaatgca gaaccaaata tttcaccaca caatctactt ataacaccca ttcctgacat
     2221 tgacattgtt attattggtc tatctgcata aattttaaac atttcatttg tagcttcaag
     2281 tagcactaac acatcttttt cattttgcgg cataacagct attttgggta aatctgcgcc
     2341 caattcctgc attctacata aacgagatac aatctcttct ttttttggag ttttattaaa
     2401 atcatgatta gatataatta ctttcacttc ttttttatga gcaaaattaa ctacttcatc
     2461 tatgacctca tctcccataa ataactctac atctattaaa tctactaaac ctgtattaga
     2521 aatttcttta ttcaaagtag tataataatc tcttgatatt aatttttctc ccccttctac
     2581 tacacttcta aatgtgaata ataaagggat atcatgtata taacttctta attcatagag
     2641 aacttcctta acttctttaa tattttctac attctcaaaa aaatcaactc gccactctat
     2701 aatatccaaa catgcatctt ttaactcttt agcctctttt ataatatctt ttttattttt
     2761 cccaataatt ggtacacaaa ttttaggtct accttctcca atagtaatat tttttacttg
     2821 aacttttctt ttcatattct cctccataat cagaaattct ataggacttt aaattctatt
     2881 ttctgcacaa tttaattata cattaaaaat actatacatt ataaaaatac taatttaaaa
     2941 ataattttta ttctaattct taaaacaatc ccacatattt tagaaaaggt actctataat
     3001 atacaaacac ttctttttta cctaaattta ttttaacatc ctataatttt cacaatattc
     3061 tttaaatttt actcatataa acaatattat tctggatgct aatagaatta atctataaat
     3121 catagtataa ttatgcagaa ataactcgaa tttcactact tatgaagaga ttgaatatta
     3181 atgaatctaa atcatttgca ttacttaaga gttttgacta aagttgaaca ccatacaaaa
     3241 gtagctactc aattgtttat accccaacca agcttaagta atgttatata gattctcgaa
     3301 aaagaatttg gtatcttttt atttgaaaaa cttatattaa atttgattat cttttcataa
     3361 gttggtaaat gtcgatatat cctgtatcta caaggagtat cggcattttc tttttggaag
     3421 atactttgca taagttggca tttaaccgta taagaatgcc atcaaaagta gtaaataagt
     3481 agtaaactct agtcatacta ctaggattat aaccgttcca tttcagctct cgaagaacta
     3541 aatgaaccat gtgcataata gccaagcgtc atagtaatgt ttttatgccc cataatatat
     3601 tgcaatatat taggactcat tcctttgtta gccatatttg tacagagtgt atgcctaaag
     3661 gtatgtggcg taatctttgg tagcttgtcc tcatgggtct tattatactt cttgactaac
     3721 gctcttaaca tgctttgata atgagttgct tgttttggta atccatcttg tttaagaaat
     3781 aggaagttct ggtaaccatc aacaatgatt ggttctgctt tagcgttctt acgccgttcc
     3841 acgatacgtt caaatgattg ataagctctt tcagtcattg gaatttctcg cttaccctgt
     3901 gtggtctttg gaacttctac ataatagcca atcttagtat cactcaacaa ttgatggtct
     3961 accataatgg ctttattttt catatccaat tctaaagtca aaccgcataa ttcagaaatg
     4021 cgtagtcctg tttctaagag gataacaaca tcatcataat atttacaata agtcttatcg
     4081 ctctccataa aggataataa gcccatttcc tgttctggtg ttagagcgat ttttggttct
     4141 gttgtatctt ccaaaacatc acttaactga aaattaaatg gattctttct aatacagtcg
     4201 tcttgaattg ccatgaaaaa cgacgctttc agtgagcgct tataattgtc aatggattta
     4261 taagcacacc catcttcact cattcggata gcccagcttt tagcgtctga taatttaacc
     4321 atatcaatgc tttttgcacc tagtgggtca ttgatagtat ctatattttg atttataatt
     4381 tatacatttt tttaaaaaca gacttttaac tgtaaagaat ctatgtataa acttagctat
     4441 gttgtccgtg tatctacaaa ggatcttgac gagctaaaaa ggcgttgtga tgaagtcaaa
     4501 gacttttacg acgacttatc actcaagctc gtgcgtccct ttggggatat gttgggattg
     4561 catggcgaat ttattcctgt cagcaaacgt tacatcaatg attatattca atatgtcact
     4621 tcggattttt tggctggact tggttttgga gcgacacaaa cccttggcga acatgaggat
     4681 atctatcttg gctacaatct ggacactgga aagaatgtct atctaaaacc tgcccttgct
     4741 agtcaatggg taaagggttc tattaccaat gtgttagcgt ctgcgtttat cggttcattg
     4801 ggtggtggta aattattcag taacaatatg cttgtttact atgcggtact attcggcgga
     4861 caggcggtca tagttgaccc taaagcagaa cgcggcaaat ggaaagaaac gctaccagaa
     4921 atagcccatg agataaacat tgtcaattta accagtaatg atgaaaacaa aggattgctt
     4981 gacccttacg tcattttgag ccgtaaaaat gattctgaaa gtttagcgat tgatatccta
     5041 acattccttg ctggtatctc cagtcgtgac agtgataagt tcccagtgct tagaaaagca
     5101 atccgcaatg taacccaaag tgaacagcga gggttattac ttgtcattga tgaattacga
     5161 aaagaagata cttctatcag taacagcata gccgaccata tcgaaagttt tgtagactat
     5221 gattttgcac atctactgtt tggtgacgga aatgtgaagc agtctatcag cttggagaaa
     5281 cagctgaata ttattcaagt tgctgacctc gtattgccag atgccaacac tagctttgaa
     5341 gaatatacaa caatagaact tcttagtgtt gctatgttga ttgttattag tacctttgca
     5401 ttagacttta ttcatactga ccgctctagc ttcaagatag ttgaccttga cgaagcatgg
     5461 tcttttttgc aggtggcaca aggtaaaacg ctatctatga aactggttag agcatcgcaa
     5521 gattcacagg acaaacgaat tgaacgtttg aagaattatc tgactgaaaa cctacaacag
     5581 ttgaatactg aaatggttcg ctcatatatt cctactagtt ctattgtcaa cagctttctc
     5641 agtttgagct tactttgcag aaaaatgaca actggaaaat aataatggaa tagtttctat
     5701 gtaaatatat ttttactatc ttacctaact aattgaaaga tgatataatt taatcaaaga
     5761 tttggtaagg agggatgaat atgcatttaa ttgattttat gaatgaagat attaaaaaga
     5821 agcttgttaa aaaaacgtat aaaaagaaag agactattct ttttgctgag caagaaaatg
     5881 aatatgtaat tcttatgatt gacggtattg cagaagcatt catcttaaat gtgaaaggaa
     5941 acatctcaac tattcatctt tatagaagtg gtagtttttt tggagaaatg gaacaattta
     6001 atgatgggaa aaaacccgtt gaaattgtcg ctttcacaga ttgtaatgta tataaacttc
     6061 atagagatga ttttttaaac tggttaaggg gagattttga agctacaaaa tttctgattc
     6121 gagaaatatc tagtaaatta gttgtaaatg ctgaattaat tgaagaatta tcatctttaa
     6181 gtgttaaaga acgacttctg aggtgtatat ccttacacta ttatagaagt gaattaaatc
     6241 tgcttaccaa aaaacagctt gctagcgaag taaacgctcc aattagaagc attaatcgtg
     6301 ctataaatga atgtgtacaa gaacatctaa ttgcttatga aaacaaaaga tttagtgtaa
     6361 ttaatcagcg agaggtatta aagaatcttc ctacaacata agtataattt caaaaaagta
     6421 ttgaataata attattcaat gcttttttat ttcgggtcaa gtgacctatt tttttttatt
     6481 ttttcattat ataatactcc tatgcataat tatgttggat tgatagctaa taaaagaaag
     6541 tgagaggtgt tatactaaaa aagaagcata cttcccaact ccagacttac agtggattga
     6601 agatagattt tggatttggg ttcattacgc ttgcactaag attggacgta gagaactatt
     6661 tgaaacaatt gattttttat cttttttaag acaatcagta cttgccccat taatacagct
     6721 tctcttaggt aaattacctc gtggagttag aaaaattgaa ttggacgcac cccaatatat
     6781 tgaagcatta gaaaagacgg ttgctaacca tgaatataaa agctgtgtta atgcattgca
     6841 ggctactatc aacctgtatc ttgagttaag agaggaatta tcacaaagtc ctattgtccg
     6901 aaaggttgaa gctgaaaggg ttgcattaca gtattttcaa gatttaatca aataaaccca
     6961 aagtaaaaaa atattgaata gtaaatattc agtgtttttt catttttggt caaatgacct
     7021 agattagttg ttgtaaccat tctataatta ttttgaaagt aacagtatgg aggataacta
     7081 tgagtgatat tttaagtaga attacaaata aaattgggga taagaatata gttgataagt
     7141 tatcagctct atcaaaatca gatttgaact cacttctatt agaagtgttt gatagacaag
     7201 caaatacttt aactgcaact gatattctaa agtcatacca actaaataga tttactatcc
     7261 ctagcggtat agatccagaa gaaattcatg ctttggagtc aaaattattg agaaaagcat
     7321 tcaatatgga tatcaagacg attatgcttt ctccctctgc tccattaggt agctgtagcg
     7381 tttttggctc cgtagatcaa tataatgtag taagtgctct acgagggaca gaaacacttg
     7441 cagacccctc aaatatgttg gcaataatta ttgctgataa attaaaaaga aaagaagaaa
     7501 ataataccat acctatccac atggctacta ctgctagggt aataagagca caaaattttg
     7561 taggtaaagg tttatattca cattttggct tatattgtat ggtttcatct ggtatagata
     7621 cagggtctta cacttgtgaa aaaatgttgt tagaaaagca cttaaactat tataaagata
     7681 tattaagtga aatagaaaat gttcgtttct ctattattct acgcaaaaga aacggataca
     7741 aagataatga cggatttttc gacagaatgg ttgaaactat caaacttatt tttcctaata
     7801 tagagttatc aattcataat gatgatgttg ataataatta ttataaaggt atcaatttca
     7861 aattatcggt caaacaagga aatgaaaccg ttgaaatagt tgacggtgga tttgttgact
     7921 ggacttatca aatgcttgga aataaaaaag aacgctgttt gataagtggt attggcttag
     7981 aaagatttct tgtagctgtt tcataaaagg agaagaaaaa tggaacttat agagtggaat
     8041 tatgcttata ttaccgattt agttgagttt gcgaatgata aagaaattgc aaagaatctt
     8101 cgtgacaact tcccccaccc ttatagggag caaaacgcta gagattttgt tagtttttgt
     8161 ttggcaactc cagatgttaa acaacttagc tatgctattt tttatcaaga aagggctatt
     8221 ggaagtatca gcctaacatt tgaagaagat acctctatta ataaaaatgc tgaattaggt
     8281 tactggcttg ctaggaacta ttggggcaaa gggatagcaa caaatgcagt aaaagaaata
     8341 tgcaaaattg cctttgaaaa atacaacctt aacaaaattc atgctaatgt atattcctac
     8401 aatagagggt caagtaaagt attggaaaaa tgtggtttta agattgaaga atactgtcac
     8461 aattcataat aaagaataga aacatgattg attgccatgt atatagttta attcgaccgc
     8521 aatagcattt attagaatta acctactttt taataacaga ataagttttt cccctctatt
     8581 aatttcttat gcatctttgt atttttatga aaagctagcc aatgcatatt tccatacatc
     8641 aatatggtgg tggcttacaa ccaatataaa ttaaatacgt tttaaagaat acacattttt
     8701 gccctatagg cagagtgtgt atttttttct cttttttttg aaatttatcg aagatttaac
     8761 gccctcccac cgtgatagga gtaagcaagg ttatttaact ccttgcgatg gaaagagggt
     8821 gaaatcttat ggttgatgaa aagacaattg aacatcaatt cgacagtttc tgtaaaacag
     8881 ttttgcgtaa ctatgctaga gatatttatg acgaaaataa acgcagaaat gattatttgg
     8941 tatctctcga atctttgtca cttgcagaat taagtaaatt gagtatttta gatgattatg
     9001 attctaacta catctgtatg gtttcttacg actacaacat acgaattgaa gatgtattaa
     9061 tggcacaagc aatcggaaaa cttacaaaaa gaaaacaaga tataattcta ctttcattct
     9121 ttctcaacat gacaaatgct gacatagcaa cattgatgga tttagcagaa aataccatac
     9181 attatcacaa aacaaatgca ctaaaagagt tgaaagaact catggaggaa cattgaaatg
     9241 aaacctacta aagaagtatt accttttaca attattttac ttgctgtaca tggcgatgta
     9301 aatgcaataa acggaatact taaacatttt gaacactata tcattcgtct ttcacagaaa
     9361 actttatttg atgaatacgg aaatccctat attcatgtag aagaagaaac aaagcgaatg
     9421 ctaggaacaa aactaatcac tgctattttg agatttaatt tgtcttaatc tcgtcctctc
     9481 tccatgtatc aagctatttg gtacatggag aatcactaat tatttgtcta taaaagccca
     9541 taagctattg tgatttttta tagacaaatc aatgccgact gttctttgac aactgaataa
     9601 agtagataga tacgtttgtg atacagatag ccaacaaata ctatacgcca tgaccttaat
     9661 caagagagct actatatagt gatttgtgaa taacctttgg aacggttgtt ttgccgtaac
     9721 ctgtaccaca gtataatgat acacctgtat gacacggttc tgcccataag aatgggattg
     9781 gttaaaatac caatggagct taccaaagcc atctatctac ttctgaacta cgaacataga
     9841 aaggagtgat acattctatg aacaatactg atgtacctat ttgggaaaaa tataccttga
     9901 cgattgaaga agcgtcaaaa tactttcgta ttggagagaa aaaattacgt aaacttgctg
     9961 atgaaaacct tgattctggc tgggtcatta tgaatagcaa tcgcattcaa ataaagagaa
    10021 aacaatttga gaaaatcgtt gatacacttg actttatcta atgaaattga gccttatatg
    10081 ttataatatt catatgacgt atcaagctca attcggttat tgagaggagc ttcttatcat
    10141 gtcaactgaa aaaagacgag ataataaaga tagaatttta agaactggag agagccagag
    10201 aaaagatgga agatacgcat ataagtatgt atattcattt ggtaaaccac aatttgttta
    10261 ctcttggaaa cttgtaccta ctgataaacc cccaaaggga aaacgtgatg atatatcatt
    10321 aagggataag gaaaaagcaa tacaaaatga ccttgatgat agcattgaca ctattggtaa
    10381 gaaaatgaca gtttgtcagt tatacgctaa aaagaatgga ctaagaaaga atatccgact
    10441 taacactaaa aaaggacgcc attatcttat gaagattctt aacaaggagt gattctgtga
    10501 agagatgtat tgtagtagtg gaatagtcaa aaacataata tatattaaag gagagattta
    10561 aatatgacta ttccaagttc aaataaggta tatccaagaa gtaatgatta tcaaactgta
    10621 tatcttaaaa atgtaataac aagggaaaat atagaagttg gagattacac aatatataat
    10681 gacttttata atgacccaag agattttgag aaaaacaatg tattgtatca ttaccctata
    10741 aacaatgata aattaataat tggtaagttt tgctcaatag catgtaatgc aaaatttcta
    10801 atgactagtg gaaatcactc aatgaaatca aaatccacat atacttttcc catcttttat
    10861 gaagaatgga aactcgatat caaccatata acggacgctt gggacaataa gggtaacatt
    10921 ataattggaa atgatgtgtg gataggttat gattctataa ttatgtcagg tgtaaatatt
    10981 ggtgatggtg caattattgg aacaagagca gtagttacta atgacgttcc accgtattct
    11041 attgttggag gagtacctgc aaaaattata aaaaagagat ttaataatga tacaatttca
    11101 aaattattag aaattaaatg gtgggattgg ccatataaaa aaattcaatc aaacattaag
    11161 catattcaat caggaaatat tgataaatta gtttaatatt ttataattta tttatattaa
    11221 actttacttt aataatcatt ttataagagc tttttcaaaa tttgtatatt attttaatat
    11281 atataaattt aatacaacat taaat
//
LOCUS       DAMJWS010000245         3286 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain NR-49300 isolate JH324
            SAMN32366166-rid16591373.guided.176, whole genome shotgun sequence.
ACCESSION   DAMJWS010000245 DAMJWS010000000
VERSION     DAMJWS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366166
            Sequence Read Archive: SRR22862991
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560847.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560847.1
            Genome Coverage       :: 169x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:09:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,908
            CDSs (total)                      :: 3,826
            Genes (coding)                    :: 3,771
            CDSs (with protein)               :: 3,771
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 24 of 55
            Pseudo Genes (incomplete)         :: 31 of 55
            Pseudo Genes (internal stop)      :: 13 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366166-rid16591373.guided.176"
                     /strain="NR-49300"
                     /isolate="JH324"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2011"
     gene            179..895
                     /locus_tag="PC399_003907"
     CDS             179..895
                     /locus_tag="PC399_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2594650.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            870..3203
                     /locus_tag="PC399_003908"
     CDS             870..3203
                     /locus_tag="PC399_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2594651.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
BASE COUNT         1382 a          294 c          564 g         1046 t
ORIGIN      
        1 atataaaaca aaatgaaaaa atattaaatt taaactagaa ggaggtttct aagatggaca
       61 ttaaatacaa ataaccagaa caaaagatat aaatatatat ataaatggaa aaacttctat
      121 catattgtat aatcatatat gtaaagaaaa tttataaatt aatataaagg agattagaat
      181 gaattcatat aatattttgg tggtagaaga tgaaaaggag atagcagatg ctatagaaat
      241 atatctatta aatcaaggat ataatgtttt taagggatac aatggattag aaggtcttaa
      301 ggttatagaa aaccaagaaa tacatttagc tatagttgat ataatgatgc ctaaaatgga
      361 tggtataaca cttacaatga agcttagaga aaactataat tttccagtaa taatgctatc
      421 tgcaaaatca gaagaagttg acaaaataat ggggcttaat ataggagcag acgattatgt
      481 aacaaaacct tttaagccat tagaactatt ggctagagtt aattcacaac ttagaaggta
      541 cactaagtat ctaaacatgg ttggaaataa agagcaaaag gtagaaagtg acgatagtgt
      601 gtttgcaata ggcggattag aattaaatga aaatacaaaa gaagttagta ttgatggaaa
      661 gtatataaaa acaactccta tagaatttaa aatactaagt ttacttatga gaaatgcagg
      721 aagagttttt tctgctgatg aaatatatga aagagtttgg aatgagaatg ctgtaaatac
      781 agatacagtt atggtacatg tgaggaatat aagggaaaag atagaaattg acccaaaaaa
      841 tcctaaatat ttaaaggtgg tgtggggtgt tggatacaaa attgaaaaaa tttaaagata
      901 aatatatagt tactattata gtttttatac ttatgttatc agcatcatta ggtatgatta
      961 gtcaatattc tactatacaa gcaagtgcta aaggtggagc agagaatcca tttgaacagg
     1021 aaagctttgt agatagtata tataaaggaa gttatgtttt atatcataat atgaaggaag
     1081 aacaagaagg taagatgata caaccatcaa agctatttat caatgaagaa actatgaact
     1141 atataaaaaa tgtaagtaaa gaaaaagata cttatgatgg ttcagaaact tattatacag
     1201 atgatagtat taatgcagat tttaatgaaa actttgaaaa ttgggagcat ttcattaaag
     1261 cgtcaggaaa gaatttaaag tactatttag ttgataaaga aagcaatttt gaatttttta
     1321 atgataataa agaattagga gtacttgata caacaaaaaa tgatgcttca aaagaaaatt
     1381 ctaatgaaaa tcagtcaaaa aatacagaaa tgaatgatga ggaaagacaa aagcagatta
     1441 cagatataaa agaaaactat agattctacg ttgtaatgaa ttttgataaa gatggaaaag
     1501 taaatattgt ggattcttat ggagtaaatc aaaaaattat taatcaaatg ttgttatcta
     1561 aagcaagaga agatttaatg gatacaagtt taggaggaaa ttcaatttca tataagttat
     1621 ctccaataaa aaatataaca ttagtatatg ctgtacctaa agtaataagt aaaggtgata
     1681 gtgtagagta tggttatgga aatacttatt ttgatgatgg tataatttca tatataagca
     1741 atgtagaaaa taattcttat tataagattt ctggaatgat tttaaaaatt atattagcta
     1801 tagttgttat tgtagcttta gcattcccat atagaaaatc taaagagttg ctaggatttg
     1861 atacattatc aagaataccg ttagagatac tctttattgc tcttgctatt atacaggggc
     1921 ttgtagattt atatcctact gttataataa gtaatacctt gagtgggaat tatgtagagt
     1981 accttataaa caatgacata agtgctaaaa tggctagtat attggtaagt ttaatgaatt
     2041 taacttattg gtttgtaata ttctcaataa tatttgtatt tataatactt ttaaaacaca
     2101 ttttaaatgt tggaataaga gagtatttta caaaacatac tgtgacagga atgttttttg
     2161 tatggttaaa aaaagcagga aaagcagtta ttaatcaaat tagaacaata aatctgaaag
     2221 aaaagccaaa taaaactata gttacaattt tgggaataaa tttattgatt atagttataa
     2281 tgtgtagtat gtggttcttt ggaataatac tcgcattaat atattcagta gtattattta
     2341 agatactgtc agattactct aaaaagacaa tatatgaata taatcaactt ttagatgtta
     2401 caaagaaaat ttcagatgga aacttagatg taaatatgga aaaagattta ggatttttta
     2461 atcctataaa agatgaatta ggaaatattc aaactggatt taaaaaagct gttgacgaag
     2521 aggttaagag tcaaaagatg aaaacagagc ttatatcaaa tgtatcacat gacttaaaaa
     2581 cacctctaac atctataatc acttatatag atttattaaa agatgaaagt ataacagatg
     2641 aaaatcgtaa gttgtatatt gatacattag acagaaagtc acaaagattg caacatctaa
     2701 tagaagattt gtttgaagta agcaaggcaa atagtggaga tgtttatttg aatatagtaa
     2761 atgtggatat tgtatctcta atgaagcaga cacttttaga attagatgac aaacttgcag
     2821 attcatctct aatagtaaag aataactttt ctaatgaaaa aataatatta ccattagata
     2881 gtcagcgtac atttagagta tttgaaaatc ttataataaa tataagcaaa tatgcaatgc
     2941 caaattcaag agtgtatatt gatattttag aaacagataa taaagtaaat attatgctta
     3001 aaaatatgtc tgcatctgag attgatttta gtgtagatga tataatggag agatttgtaa
     3061 gaggagataa atcacgtaac acagaaggtt ctggtcttgg tttagcgata gcaaaaagtt
     3121 ttatagaatt acagggtggt aagatgaata taagtgtgga tggtgattta ttcaaagtta
     3181 caattacttt taacaaaaat tagattagac ttctttaata attataaagt ctatagaata
     3241 aaagaatctg gaaatattta tgtgtattct ttaaataaaa atttat
//
